LOCUS       DASZYN010000001         5328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_835315, whole genome shotgun sequence.
ACCESSION   DASZYN010000001 DASZYN010000000
VERSION     DASZYN010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835315"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1442)
                     /locus_tag="VHE33_00005"
     CDS             complement(<1..1442)
                     /locus_tag="VHE33_00005"
                     /inference="COORDINATES: protein motif:HMM:NF006189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HVT95859.1"
                     /translation="MKTLTALRYLEDLWDEKTAATLDEPELLRYRSNLFGADLRITNF
                     GGGNTSSKVLQADPLTGQTADVLWVKGSGGDLGSIKRAGFATLYLDKLLAIEKQYKGV
                     AEEDAVVDLYPLCTFGVNPTVASIDTPLHGFLPFRHVDHLHPDWGIAMAAAANGKEKM
                     EEFNREYEHKLVWVPWQRPGFELGLMMRRAVEENPGCDGIVLGGHGLFTWGETQRECY
                     LNTLTMIDHLGQFVQDHVERKGDAIFGGAALESRPDRKELAIELLPFVRGRVSQQRRF
                     IGSWSDGDDVMRFVNSKDGAALAHLGTSCPDHFIRTKIRPMFVPAVSNDDSATLQKKI
                     EAALDTYRAEYAAYYTKHQESTSPAMRDPNPTVVLIPGVGMFSFGKSKSESRIIGEFY
                     TNAIHVMEGATALAGKITVKDFPQAGAAASCEAFEAYSNYVALPAVEAFRIEYWLLEE
                     AKLRRQPAEKELSRRIALIVGGASGIGRAAA"
     gene            complement(1579..2688)
                     /locus_tag="VHE33_00010"
     CDS             complement(1579..2688)
                     /locus_tag="VHE33_00010"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HVT95860.1"
                     /translation="MPRTSTKRLYFIPILSKALDVLEVLQKSERPMSLEAIYQRTHIS
                     KTTVYRILKTFVHRGYLAQSQDGLYRLAARPRKMRFGFGKQSGEMPFSEAVTASLYDA
                     ATQAGVDLMVLDNRYDAATAVQVAEEFVRSRVDLVIEFQIDQEVAPIIADKIAVAGIP
                     LIAVDIPHPHATYFGVDNYRVGVEAGALLAGHAVEKWNGAVDWILGLDIENAGPLVQS
                     RITGVFQGIRNQLPELPVEKFVRVDGRGMREPGHRVIADFLKRHPKDRHILIAAATDS
                     SALGALEAVRQQKRERHVAIVGQDCISEAIEEMKRPDTPWIGSVSHEAGTYGPRLIQL
                     GLSILRGLTVPPYNYVEHKLVTAKMVREAPPESKV"
     gene            complement(2777..3955)
                     /locus_tag="VHE33_00015"
     CDS             complement(2777..3955)
                     /locus_tag="VHE33_00015"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HVT95861.1"
                     /translation="MTAHLEQVSKALDSLRIELPSWGFANTGTRFGKFLQAEAAATIE
                     EKFSDAAQVHALTGCTPTLALHIAWDLPGGLRDAARIRSLEAEHGIRSGSINPNLFES
                     QDYKFGSICNPDPGIRRKAINHLVESVAIAKALRSRDLSLWLADGSNYPGSQSIRRRI
                     DWMAGALAEVHSELGEDQRLLVEYKPFEPAFYHTDLADWGMSLLIARETGRKAQVLVD
                     TGHHYQGQNIEQIVVWLMREKMLGGFHFNDRRYADDDLTLGSIDPYQVFRIFHELLSE
                     DGAMETTAFMVDQSHNLKGKMEAMVQTVCTAQEIFAKAALVDRERLATLQDECRLVEA
                     EECLRQGFWFDVRPLVHEWRKVRGLPEEPLVALRESGYVERIRNERKERNARSVTTYA
                     "
     gene            4088..4408
                     /locus_tag="VHE33_00020"
     CDS             4088..4408
                     /locus_tag="VHE33_00020"
                     /inference="COORDINATES: protein motif:HMM:NF017174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HVT95862.1"
                     /translation="MTRVCFLLRVRAERLAEYRERHKAVWPEMLDALQATGWHNYSLF
                     LRPDGLLVGYLETPDFERARAGMQNHPVNARWQAEMAPFFESLGNGTADASMMPLEEV
                     FHLD"
     gene            complement(4443..>5328)
                     /locus_tag="VHE33_00025"
     CDS             complement(4443..>5328)
                     /locus_tag="VHE33_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT95863.1"
                     /translation="ARGVFRAAVAIVFSCCLHAGGQTPDSAPPATPNLTGIAHVAIRV
                     ADLERARSFFHQLGFEEAFAMDQGGKPTEAFFKINDRQFIELYPRRDASSAVGFMHVC
                     FEAADLNAVHQDYVSHGLSPNQVRKAGAGNLLFTLQGPDQPGPPSDLRGAATQNIEYT
                     QYMPGSRHTLDRGQHLGPNRIADRIAGVGIPFADPAAAETFYIDKLSFERSDRSLVPG
                     YPTLALPSSPGQQIEFLPATVATPSALPFDIIFSVPNLRSTQKRLNSLGIAAMRHQKA
                     VTIQDPDGDSIVFVEAHE"
BASE COUNT         1100 a         1543 c         1584 g         1101 t
ORIGIN      
        1 gcagcggcgc gtccaatgcc acttgcgcct ccgacaatca gagcaatacg gcggctgagt
       61 tctttttccg ccggctggcg gcgcaacttg gcttcttcga ggagccaata ttcaatgcgg
      121 aaggcctcca ctgcaggcag cgcgacataa ttggagtaag cctcaaaggc ctcacacgat
      181 gcggcggctc ccgcctgggg aaagtctttc acggtaatct tgccggccag tgctgtagcc
      241 ccttccataa cgtgaatcgc attcgtgtag aactcgccga tgatgcgcga ctcgctcttg
      301 ctcttgccga aggaaaacat gccgacgccg ggtataagaa ccacggtcgg attcggatcg
      361 cgcatcgccg gcgaagtcga ttcctgatgc ttcgtgtagt aagcggcgta ctcggcacga
      421 tacgtatcga gcgcggcctc gattttcttc tgcagcgttg cggaatcgtc attcgagaca
      481 gccggaacaa acatcggccg aatcttggtg cggatgaagt gatccggaca gctggtgccg
      541 aggtgcgcga gagccgcgcc atccttcgaa ttgacaaagc gcatgacatc gtcgccgtca
      601 gaccaactgc cgatgaatcg ccgttgttgc gagactcgcc cgcggacgaa gggcaacagc
      661 tcaatggcga gctctttccg gtcgggacgc gattccaacg cagcgccgcc gaagatcgcg
      721 tcgcctttgc gctcgacgtg atcctgcacg aactggccta ggtggtcgat catggtcagc
      781 gtgttcagat agcactcacg ctgcgtctct ccccatgtga acaagccatg gccaccgaga
      841 acgatgccat cgcagccggg attctcctcg accgcgcgac gcatcatcag gccaagctca
      901 aatcccgggc gctgccacgg cacccatacc agcttgtgct cgtactcgcg attgaactcc
      961 tccatcttct ccttgccgtt ggcggccgct gccatggcga tgccccagtc cggatgcagg
     1021 tggtcgacgt gacggaacgg aagaaagccg tgcagcggcg tgtcgatgga ggcaacggtt
     1081 gggttcacac cgaaggtgca gagcgggtaa aggtcaacga cagcgtcttc ctccgctacg
     1141 cctttgtact gcttctcaat cgccagcagt ttgtccaggt acagcgtggc gaaaccggcg
     1201 cgcttgatgc tgcccagatc gccgccgctc cctttgaccc acagcacatc ggccgtttgg
     1261 ccggtcagcg gatctgcttg caggactttg gaactggtgt tgccgccgcc gaagttggtg
     1321 atgcgcagat cggcgccaaa caggttggag cggtagcgca gcaactcggg ttcatccagc
     1381 gtggccgccg ttttttcgtc ccacaaatcc tcgaggtatc gcagtgcagt cagcgtcttc
     1441 atcgattcac cgtcccaaat aatgacatag cgaatatcaa tagagcaatt gtttcagatt
     1501 ttgaatatcc gaacctagtt ccagctcgaa attccggctg aaccaaaaaa tttcgtgccc
     1561 gatgggtggg ctaaggccct aaactttcga ctctggcggg gcctcgcgca ccatcttcgc
     1621 cgtgaccagc ttgtgttcca catagttata cggcggcacg gtgaggccgc gcagaatcga
     1681 gagtcccagc tgaataagcc gggggccata ggtcccggct tcatgggaga ccgagccaat
     1741 ccacggcgta tcaggacgct tcatctcttc aatggcttca gaaatgcagt cctggccaac
     1801 aatggcgacg tgcctctcgc gcttctgttg gcgaacggct tcgagggccc ccagggcgct
     1861 ggaatccgtg gcagcagcga tcaggatgtg ccggtctttg gggtggcgct tgaggaagtc
     1921 ggcaatgacc cgatgaccag gctcgcgcat accccgacca tccacgcgca caaacttctc
     1981 aacaggcagt tccggcagct gatttcgaat gccctgaaaa actcccgtga tgcggctttg
     2041 cacaagagga ccggcattct cgatgtcgag tccgagaatc cagtccacgg caccgttcca
     2101 cttctccacc gcatgtccag ccagcagagc gccagcttcg actccgacgc gatagttgtc
     2161 cacaccaaag taggtggcgt gcgggtgcgg aatgtcgacg gcgatgagag gaatgcccgc
     2221 gacggcaatc ttgtcggcaa tgataggagc cacttcctgg tcaatctgga actcaatgac
     2281 caggtctacg cgactgcgga caaactcctc cgccacctgc acagcagtcg cggcatcgta
     2341 gcggttgtcc agcaccatca gatcgacgcc ggcctgagtc gcggcatcat acaaactcgc
     2401 ggtcaccgct tcggagaagg gcatctcgcc cgactgctta ccgaacccga atcgcatctt
     2461 tcgcgggcgg gcggccaggc gatagagccc gtcctgcgac tgagccagat agccccggtg
     2521 aacgaaggtc ttgaggatcc ggtaaacagt ggttttggag atatgtgtgc gttggtagat
     2581 ggcttccagc gacatcggcc gctcgctctt ttgaaggacc tccagaacat cgagagcctt
     2641 ggagagaata ggaatgaaat acaggcgctt cgtggaagtt cttggcaagg agcagcctca
     2701 gtcaaacatc acggcagtta tatcatcggg gtttcaatcg ggaaaccgcg agcacgactc
     2761 tgaaacctga agccgattac gcgtaagtag tgacggaacg cgcgttacgc tcttttcgct
     2821 cattgcgaat gcgttcgacg taaccgcttt ctctgagcgc cacgaggggt tcttccggca
     2881 atccacgcac tttgcgccat tcatgtacca gcggtcgcac gtcgaaccag aagccctgcc
     2941 gcaggcattc ttcagcctcc accaggcggc attcgtcctg cagcgtggcg agtcgttctc
     3001 gatcaaccag cgccgctttt gcgaagatct cctgcgccgt gcaaacggtc tggaccatag
     3061 cttccatctt gcctttcaga ttgtggctct ggtcaaccat gaatgctgtg gtctccatgg
     3121 cgccatcttc agatagcaac tcgtggaaga tgcggaagac ctggtatgga tcgatcgaac
     3181 ccagcgtcag atcatcgtcg gcatagcggc gatcgttgaa gtggaaaccg ccaagcatct
     3241 tctcgcgcat gagccatacg acgatctgtt caatattctg cccctgatag tggtggccgg
     3301 tatcgaccag cacctgagcc tttctgcccg tctctcgcgc gatcagcaat gacatgcccc
     3361 agtcagcgag gtcggtgtga taaaaggcgg gctcgaatgg cttatattcc accagcagcc
     3421 gctgatcctc gcccagttca ctatgcactt cggcaagcgc ccccgccatc cagtcgatac
     3481 gacggcgaat gctctgcgag ccgggatagt tcgacccgtc cgccagccat agtgaaagat
     3541 cccgggaacg gagcgccttg gcgatggcga cggattccac gagatgattg atggccttcc
     3601 tgcggattcc ggggtccgga ttgcagatgg atccgaattt gtagtcctgg ctctcgaaaa
     3661 ggttcggatt gattgaaccc gagcgaatgc cgtgctcggc ttcgaggctt cgaatcctgg
     3721 cggcatcgcg cagcccgccc ggcagatccc atgcgatgtg cagcgccagg gttggagtgc
     3781 atccggtaag tgcgtgcacc tgcgcggcat cagagaattt ctcctcgatt gtggctgccg
     3841 cctcggcctg caggaatttg ccgaaccgag tgccggtgtt tgcaaagccc caggagggca
     3901 actcaatccg cagcgaatca agagctttgc tgacctgctc caggtgtgcc gtcaccgctg
     3961 tggttctccc atttgaaata agttcccagt taaaatgcca tggtactgcc cgctaatcga
     4021 ggcgtcaaag gaagaattgt ggagcggcga gagttctgat ttcttgagcg aaatcggggg
     4081 gtgtgttgtg acgcgtgtgt gttttctttt gagggttcga gccgagagac ttgcagagta
     4141 tcgtgagcgc cacaaggccg tttggccgga gatgctggac gcgctgcagg ccacaggctg
     4201 gcataactac tcgttgttcc tgcggcctga cggtcttcta gtcgggtacc tcgagacgcc
     4261 ggattttgag cgggctcgcg ccggcatgca gaaccatccg gtcaatgctc gatggcaggc
     4321 ggaaatggcg ccgttctttg agtcgctcgg gaacggaaca gccgacgcca gcatgatgcc
     4381 gctggaggaa gttttccacc tcgattgaga tcggaattga gggagccaaa tgcgctcatc
     4441 gttcattcgt gcgcctcgac aaacacgatg gaatcgccgt cgggatcctg aatcgtcacc
     4501 gccttctggt gtcgcatcgc ggcgataccg agcgaattaa gccgtttctg cgtgctacgg
     4561 aggttgggaa ccgaaaagat gatgtcaaac ggcagcgcgc tcggcgtagc aacggtcgca
     4621 ggcaggaatt caatttgctg gccgggagat gagggcagcg cgagcgtggg atatccgggg
     4681 acaagggacc gatcgctgcg ctcaaacgaa agcttatcga tgtagaacgt ctctgcggct
     4741 gcaggatcgg cgaagggaat gccgacgcct gcaatgcggt cggcgatgcg gtttggaccg
     4801 agatgctgac cgcggtcgag cgtgtgtcgc gatcccggca tgtattgcgt gtactcgatg
     4861 ttttgtgtcg cggccccgcg taaatccgac ggtggccccg gttgatcggg tccctgcagg
     4921 gtgaagagca gattgcctgc tccggccttt cggacctgat tgggcgaaag tccgtgactc
     4981 acgtagtcct gatgaaccgc gttcaaatcc gcggcttcga agcacacgtg catgaaccca
     5041 accgcggaag acgcatcgcg tcgcggatac agctcaataa actgccggtc attgatcttg
     5101 aagaatgcct ctgtcggttt gccaccctgg tccatggcga aggcttcttc aaatccgagc
     5161 tgatggaaga atgagcgcgc ccgttcgagg tcggccacgc ggatcgccac gtgcgcgatg
     5221 ccggtgagat tgggtgtcgc cggtggagcg gaatccggag tctgtccgcc ggcgtggagg
     5281 cagcaactga acacaatcgc aactgcggcg cgaaaaacgc cgcgtgca
//
LOCUS       DASZYN010000002         1778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_128643, whole genome shotgun sequence.
ACCESSION   DASZYN010000002 DASZYN010000000
VERSION     DASZYN010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128643"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..922)
                     /locus_tag="VHE33_00030"
     CDS             complement(116..922)
                     /locus_tag="VHE33_00030"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HVT95864.1"
                     /translation="MFRSRLARLTLCVALTALCVAPILRAQEQPPEEGPRRGFGMGPG
                     GGMFGPGSGGTMGKVTAISGTELTIQDDQGQTFKIETGPNSRIRKNREEAKIADIKIG
                     DTVIAGGNLDEQAKTIGAMFVMVLDPQQAAEAEKRRATFGKTWTAGRITAIKDLTLTI
                     ERPDKVTQTVAVDENTTFHKGTRGQATDITFPDIKVGEMMHAEGAVQGNNFLATNVVV
                     MEPRQPGERRFGNGGPPSAGGSGSRMRQQPQPEGTPAGSTTPPQPQAGQN"
     gene            1217..>1778
                     /locus_tag="VHE33_00035"
     CDS             1217..>1778
                     /locus_tag="VHE33_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HVT95865.1"
                     /translation="MSVLATTVLAEEFCRVRSQSLALCAPLTPEDMMVQSCADASPAK
                     WHLAHTTWFFETFVLREFVPAYKPFHKDFIWLFNSYYNAVSEQPEKKLRSSFSWPRAE
                     EILAYRHHVDAAMEDFAAGEIPEAARERIVLGLNHEQQHQELLITDVKHAFWSNPLHP
                     AYIQGTDAIRGAGGAAPLRWVEFAGGM"
BASE COUNT          307 a          509 c          615 g          347 t
ORIGIN      
        1 tcaggattgg gcgggttggg cgacgccggc tccggcgatt gggcaaagcc gagagatccc
       61 gcaaggagca gcgagacaac aagagcgggg atacgacggg ggcgcgtcat cggcgtcagt
      121 tctgcccggc ctggggctgc ggcggcgtcg tcgatccggc aggagttcct tcgggttggg
      181 gctgctggcg catgcgcgat ccagaccctc cggccgaggg cggcccgccg ttgccaaaac
      241 gccgctcgcc cggctgccgc ggctccatca cgactacgtt ggtcgcgagg aagttgttcc
      301 cctgcacagc gccttcggcg tgcatcattt cgccgacttt aatgtcgggg aaggtgatgt
      361 cggtcgcctg gccgcgggta cctttgtgga acgtggtgtt ttcgtcgacg gcgacggtct
      421 gcgtgacttt gtcggggcgt tcgatggtga gggtcagatc tttgatcgcg gtgatgcggc
      481 ccgcggtcca ggttttgccg aaggtcgcgc ggcgcttctc ggcctcggcc gcttgctgtg
      541 ggtcgagcac catgacgaac atggcgccaa tggtctttgc ctgctcgtcg agattgccgc
      601 cggcgatcac ggtgtcgccg attttgatgt cggcgatctt tgcctcctcg cgattcttgc
      661 ggatgcggga gttggggccg gtttcgattt tgaaggtctg gccctgatcg tcctggatgg
      721 tgagttcagt accggagata gcggtgacct tgcccatggt gccgccgctg ccgggaccga
      781 acatgccgcc accggggccc atgccgaagc cgcgacgagg gccttcctcc ggcggttgct
      841 cctgcgcgcg gagaatcggg gcgacgcaga gtgcagtcag agcaacacaa agcgtgagac
      901 gtgcaaggcg cgagcggaac atgctgatcc tcatccagac agaggcggtg tgaggccgct
      961 ctgattatgc cggaatagac gcatttttcg ccggaaagac gcggtgaacg cctcacagca
     1021 cggcgcgaaa cctgcgggtg aggaagcgga aaaaccttcc gctgcatcca tgccgcgcag
     1081 gttaaagata cttcaagaag tgaaaattgc accctggtgg gaagctggcg catcccatgc
     1141 ccctgagggt ggtagtctga cgggcatccc cagccccgat ggctctcagc tgggaagatt
     1201 ttaaaaggga cgggccatga gcgtactcgc cacaactgtg cttgcggaag aattttgtcg
     1261 cgtgcggagc cagagtctcg cgctctgcgc gccgctgact cctgaagaca tgatggtgca
     1321 atcctgcgcg gacgcgagcc cggcgaagtg gcacctggcg cacacgacct ggttttttga
     1381 gacctttgtg ctgcgcgaat tcgtgccggc ctataagccg tttcacaaag acttcatctg
     1441 gctgttcaac agctactaca acgcggtgtc agagcagccg gagaagaagc tgcgttcgtc
     1501 gttttcgtgg cccagggcgg aggagatcct cgcgtaccgg catcatgtgg acgcggcaat
     1561 ggaggacttc gcggcgggcg agattccgga ggcggcgcgc gagcgcattg tgctgggtct
     1621 caatcacgag cagcagcacc aggagctgct gatcaccgac gtcaagcacg cgttctggtc
     1681 gaatccgctg cacccggctt atatacaggg aacggacgcg attcgcggcg cgggcggggc
     1741 tgctccgctg cgctgggtcg agtttgccgg cggcatgg
//
LOCUS       DASZYN010000003         4506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_193041, whole genome shotgun sequence.
ACCESSION   DASZYN010000003 DASZYN010000000
VERSION     DASZYN010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193041"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="VHE33_00040"
     CDS             <1..1439
                     /locus_tag="VHE33_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVT95866.1"
                     /translation="VTMLIESSPASLDPRIGTDGQAEHIDSLLFDPLVRRDERFGLQP
                     ALAIKWEIPDPRTWVFHLRTDARFHDGRPLTSRDVKYTIDSILDGTVISVKAGSFTTL
                     DRIDAPDPATVIFHLKQPDPSLPFNLCDGAIGIVPYGSGRDFFRHPIGSGPFTFVSQQ
                     LDRDVVIARAPSYWGPLPNIPRVRFAVVPDATTRALELKKGSADVESNALPADEVNAL
                     ARDSHLAMDDGPGTVLNYIVFNTRDSILRDVRVRTAIALAINRPLLIQALYRGQARLA
                     ESLLPPQHWAWVSTPPHNYDPAQANALLDQAGYPRNAHGIRFHIGMKTSNDETVRLTA
                     VAIQEQLAQIGIALDLRSYEFATFYADLARGVFQLAPGRWIGGNENPDIFRYVYATAS
                     FPPNGGNRGFYSSPEMDRLIADAASTQAAQQRLADYARIQQLAAHDLPDINLWYLDSV
                     IVHNKRLGNIHPNPSGTFDFLRTATLQR"
     gene            1650..2264
                     /locus_tag="VHE33_00045"
     CDS             1650..2264
                     /locus_tag="VHE33_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HVT95867.1"
                     /translation="MPLTLPEGPFSAYLFDMDGTIADSMPLHYIAWKQALAEYGCSDF
                     PEDLFYSWGGMPVREIISTLNQRHALSMPVEEVARRKEGLYYELLPQLTAVPEVLEQI
                     EEAHQRIPFAVVSGSTRDSVTASLRALHLENKFDVLVCAGDYTNAKPDPEPFLLAAKK
                     LNVPPASCLVFEDTAMGIEAATRAGMQSVRILQPWERPKAQPVG"
     gene            complement(2272..3771)
                     /locus_tag="VHE33_00050"
     CDS             complement(2272..3771)
                     /locus_tag="VHE33_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HVT95868.1"
                     /translation="METRRTVLKYLAGAVGSAACGLDALETLALEAGDHSKKPNIVCI
                     VGEGLRWDEFSSAGNRLLQTPNMDRIGREGCTFRNAFVVNALCLPSRATMLTGMYSHT
                     TGAVSNVEGKIAPRLPLVSDLLQKAGYETAFLGKSHVEGALMDHDWDYYFGFVGQADY
                     YRPRITEGVKGKYGPAKLYAGQYVDTLLTQKAVEWLAQERTKPFCLFLWFYAPHAPFY
                     RPKDMVNDFNGVPIRKPATFDEDLKGYPGKPRAVADADNKIGYSEVFSDDPRSLEELV
                     KDHYAGVEDNDRNVGAIWKELERQKITEETAFLLSSDHGFFLGEHHFYDKRLMYEPSI
                     RVPMMVRAPGRVKAGTAKEEMVLNLDMAPTLLDIAGVAPPEMQGKSMMPLAEGRSVAW
                     RKDWLYEYYEYPGFENVRPCRGVRTERYKYIHFFLEPQEYELYDLEKDPDEANNLYGK
                     AGYEEITAHLKKRLDELRAETNDTYEYKPTGLPLHFDPGVQTESQLRPK"
     gene            3880..>4506
                     /locus_tag="VHE33_00055"
     CDS             3880..>4506
                     /locus_tag="VHE33_00055"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HVT95869.1"
                     /translation="MRRVSSIPMQTGFHVMTKPIGPICNLDCSYCFYLEKEKLYPGTR
                     QWAMAPAILERYIRQYIAAQPLDEIHFAWQGGEPTLLGIDFFRTVVELQQKYAAGKTI
                     HNALQTNGTLLDDAWCEFFADNDFLVGLSIDGPRELHDSYRVDKGGAPTFDRVLRGLN
                     RLKAHQVDFNTLTVVNRRNSEHPLEVYRFLRDTGSGFIQFIPVVERKAA"
BASE COUNT          828 a         1749 c         1076 g          853 t
ORIGIN      
        1 ccgtcaccat gctcatcgag tccagccccg cctccctcga tccccgcatc ggcaccgacg
       61 gccaggccga gcacatcgac tccttgctgt tcgatcccct cgtccgtcgc gacgagcgct
      121 tcggcctcca gcccgctctc gcaattaagt gggaaatacc cgacccgcgc acctgggtct
      181 tccacctccg caccgatgcc cgctttcacg acggccgccc cctcacctcg cgcgacgtga
      241 agtacaccat cgactcgatt ttggatggaa cggtcatctc cgtgaaagcc ggctccttca
      301 cgactcttga ccgcatcgac gcgcccgatc ccgcgaccgt tatcttccac ctcaagcagc
      361 ccgatccctc gctccccttc aacctctgcg atggcgctat cggcatcgtt ccgtatggat
      421 ccggccgcga cttctttcgt cacccgatcg gcagcggccc ctttaccttc gtcagccagc
      481 aactcgaccg cgacgtcgtc atcgcccgcg ctccgtcgta ctggggaccc cttcccaaca
      541 tcccgcgcgt ccgcttcgcc gtcgtccccg acgccaccac ccgcgccctt gaactcaaaa
      601 aaggctccgc cgacgtcgaa agcaacgcgc tccccgcgga cgaggtcaac gccctcgccc
      661 gcgattcgca tcttgctatg gatgacggac ccggcacggt cctcaactac atcgtcttca
      721 acacccgcga ctcaatcctg cgtgacgtcc gtgtccgcac cgccatcgcc ctcgccatca
      781 accgcccgct gctcatccag gcgctctacc gcggacaggc gcgcctcgcc gaaagcctgc
      841 tcccgccgca acactgggcc tgggtctcca cgccgcccca caactacgac cccgcccagg
      901 ctaacgccct cctcgaccag gccggctacc cgcggaacgc acacggcatc cgcttccaca
      961 tcggcatgaa gacctccaac gacgaaaccg tgcgcctcac cgccgtcgcc atccaggagc
     1021 agctcgccca aatcggcatc gccctcgacc tgcgcagcta cgagttcgcg accttctacg
     1081 ccgacctggc tcggggcgtg ttccagctcg ctcccggccg ctggatcggc ggaaatgaga
     1141 atccagacat tttccgctac gtgtacgcca ccgccagctt cccgcccaac ggcggcaatc
     1201 gcggctttta ttcgagcccg gagatggacc gcctcatcgc cgatgccgcc tcaacccagg
     1261 ccgcgcaaca gcgcctcgcg gactacgccc gcatccagca actcgccgcc cacgatctcc
     1321 ccgacatcaa tctctggtac ctcgactcgg tcatcgtcca caacaaacgc ctcggcaaca
     1381 tccacccgaa tccctccggc accttcgatt tcctgcgcac cgccacgttg cagcggtgaa
     1441 ataaggacaa actaacaacc agcgaatacc gggctgagtt cgagcgaaaa aacgagctgt
     1501 tttcctggct cttcactcgc tttctcgaag cttgctcgct ggtcactcgc gttttgcttt
     1561 gcgcctctac ccagcttttc tctcgattct ggctcgatat ccgcgcgcac ttggctcgct
     1621 tctcgcttcg tctccgttac cctgaaacca tgcccctcac gctccccgaa ggccccttct
     1681 ccgcctacct cttcgacatg gacggcacca tcgccgactc catgcccctg cactacatcg
     1741 cctggaagca ggctctcgcc gaatacggct gttccgattt tcctgaagac ctcttctact
     1801 cgtggggagg catgcccgtc cgcgaaatca tctccacgct caaccagcgc cacgcgcttt
     1861 cgatgccggt cgaagaggtc gcccgccgca aggaaggcct ctactacgaa ctgctcccgc
     1921 agctcacagc cgtccccgaa gtcctcgagc agatcgaaga agcgcaccag cgaatcccct
     1981 tcgccgtcgt ctccggttcc acgcgcgact ccgtcacagc ctcgctccgc gccctccatc
     2041 tcgaaaacaa gttcgacgtc ctcgtctgcg ctggcgacta caccaacgcc aagcccgacc
     2101 ccgagccgtt tctgctcgcc gcaaagaagc tcaacgtccc acccgcatcg tgcctcgtct
     2161 tcgaagacac agcgatgggc atcgaagccg ccacccgcgc cggcatgcaa tcggtccgca
     2221 tcctccaacc ctgggagcgc ccaaaagcac agcccgtcgg ttgacggccg cctacttcgg
     2281 ccgcagttgc gactccgttt gcactcccgg atcgaaatgc agcggcagcc ccgtcggctt
     2341 gtactcgtac gtgtcgttcg tctccgcgcg cagctcgtcc agccgcttct tcaaatgcgc
     2401 ggtgatctcc tcgtatcccg ccttgccgta gagattgttc gcctcgtccg gatctttttc
     2461 gagatcgtat aactcatact cctgcggctc gagaaagaag tggatgtact tgtaccgctc
     2521 cgttcgcacc ccgcggcacg gccgcacatt ttcgaatccc ggatactcgt agtactcgta
     2581 cagccagtcc ttccgccacg ccacgctccg gccctcagcc agcggcatca tgcttttgcc
     2641 ctgcatctcc ggcggcgcga cccccgcaat atccagcagc gtaggcgcca tgtccagatt
     2701 cagcaccatc tcctctttcg ccgttcccgc cttcacccgc cccggcgcgc gcaccatcat
     2761 cggcacgcgg atcgaaggct cgtacatcag gcgcttgtcg tagaaatgat gctcgccgag
     2821 aaagaagccg tgatccgagc tcagcagaaa cgccgtctcc tccgtaatct tctgccgctc
     2881 cagttccttc cagatcgccc caacattgcg atcgttgtcc tcgacgcccg catagtgatc
     2941 cttcaccagc tcttccagcg atcgcggatc gtcgctgaac acttccgaat atccaatctt
     3001 gttgtctgca tccgctactg cgcgcggctt gcccggatac cccttcaaat cctcgtcgaa
     3061 cgtcgccggc ttccgaatcg gcaccccgtt gaagtcattc accatatcct tcggccgata
     3121 gaacggcgca tgcggcgcat agaaccacag gaaaagacag aacggcttcg tgcgctcctg
     3181 ggcgagccac tccaccgctt tctgcgtcag cagcgtgtcg acatactggc ccgcatacag
     3241 cttcgccggc ccatacttgc ccttcacgcc ctccgtgatc cgcggccgat agtagtccgc
     3301 ctggcccacg aacccaaagt agtaatccca gtcgtggtcc atcagcgcgc cttccacatg
     3361 cgacttgccg aggaacgccg tctcataccc ggctttctgc aacaaatccg acaccagagg
     3421 cagccgcggc gcaatcttcc cctccacatt cgacaccgcg cccgtggtgt gcgagtacat
     3481 ccccgtcagc atcgtcgcgc gcgagggcag gcacagcgca ttcaccacga acgcgttgcg
     3541 gaaggtgcat ccctcgcggc cgatgcggtc catgttcggc gtctgcagca gcctgtttcc
     3601 cgccgaggag aactcgtccc agcgcagccc ctcacccacg atgcaaacga tgttcggctt
     3661 cttgctgtgg tcccccgcct caagcgccag cgtctccagc gcatcgaggc cgcacgcggc
     3721 gctccccact gcacccgcca gatatttcag aaccgttctg cgcgtctcca tttcgtctcc
     3781 cgtccacatg cgccgaatcg tgtcgacttt caacaacctg ctgtcaacca aatcaaaaca
     3841 aagcagcatt gatagacccc cgcccgcgcg gtgtcaagca tgcgccgcgt atcatcgatt
     3901 cctatgcaga ccgggttcca cgtcatgacc aagcccattg ggccgatctg caacctcgac
     3961 tgcagctact gcttttacct ggagaaagag aagctgtacc ccggcacgcg ccagtgggcg
     4021 atggcccctg caatcctcga gcgctacatc cggcagtaca ttgccgcgca gccgctcgac
     4081 gaaatccact tcgcctggca gggcggcgag cccaccctcc tcggtatcga cttcttccgc
     4141 accgtcgtcg agctccagca aaaatacgcc gccggcaaaa ccatccacaa cgcactccag
     4201 accaacggga ccctgcttga cgatgcctgg tgcgagttct tcgccgacaa cgacttcctc
     4261 gtcggcctct ccatcgatgg gcctcgcgaa ctgcacgaca gctatcgcgt cgacaagggc
     4321 ggcgcgccca cgttcgatcg cgtgttgcgc ggcctcaacc gcctcaaagc ccatcaggtc
     4381 gacttcaaca ccctcaccgt cgtcaaccgc cgcaactccg agcatcccct tgaggtctac
     4441 cgctttctgc gcgataccgg cagcggcttc atccagttca tccctgtcgt cgagcgcaaa
     4501 gccgcc
//
LOCUS       DASZYN010000004         7804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_257216, whole genome shotgun sequence.
ACCESSION   DASZYN010000004 DASZYN010000000
VERSION     DASZYN010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7804
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257216"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VHE33_00060"
     rRNA            complement(<1..909)
                     /locus_tag="VHE33_00060"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(1229..1304)
                     /locus_tag="VHE33_00065"
     tRNA            complement(1229..1304)
                     /locus_tag="VHE33_00065"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1269..1271),aa:Ala,seq:tgc)
     gene            complement(1314..1390)
                     /locus_tag="VHE33_00070"
     tRNA            complement(1314..1390)
                     /locus_tag="VHE33_00070"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1354..1356),aa:Ile,seq:gat)
     gene            complement(1649..3148)
                     /locus_tag="VHE33_00075"
     rRNA            complement(1649..3148)
                     /locus_tag="VHE33_00075"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(3920..5521)
                     /locus_tag="VHE33_00080"
     CDS             complement(3920..5521)
                     /locus_tag="VHE33_00080"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HVT95870.1"
                     /translation="MKDAGFLPTRRQFAQGAGAAVLDSSLLARNLAGFDSPGAVAGEL
                     RIDVSAPLTPFNRMLFGQFLEHFHRQVYGGVFDPGSPLSDHRGFRIDVIEALRELKIP
                     VVRWPGGCFASAYHWRDGVGDNRQPALDKAWGVEDPNTFGTAEFVEWCRRIGAAPYIC
                     TNAGTGTQEEMSDWVEYCNLKDQGRYARMRRANGFAEPFDVRYWSIGNENYLGGEIGA
                     KTVEEWAPLVRESAKMMRAVDSGLKILAAATVNGNWTPPMLKAAGKYLDYISIHGYWD
                     PLWQRGTKPSDYLHCMARCEQPEQQIRQAVELLEQVGFGDRIGIAFDEWNLRGWHHPG
                     FPGGGADQVEMIRERDENDRNETYTMADALFSASFLNACLRNAKHVQMACMAPVVNAR
                     GPLFVHPRGIVKRTTFYVLRMYSDRLQRNVVSSQVGCGPLRVDQSSVAALDGLATCSD
                     DRRQIAVALVNRHPDQVARWKMDFGSGINLREATVTILAGGSPDDYNDVAHPERVVPQ
                     THGVTFENGLFQQPAHSIALLQASL"
     gene            complement(5754..6452)
                     /locus_tag="VHE33_00085"
     CDS             complement(5754..6452)
                     /locus_tag="VHE33_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HVT95871.1"
                     /translation="MLIQHFAVMVSPTAAAEQALNSSYVKFSLLALSSIFFLVDPFAA
                     IPSFIAITSDVEPARRRRMAWKAALTCFIVLITFALAGQYIFSLFGIKLPAFEIAGGL
                     ILLLIGIDMMQARRSPTQESQEETQEAAAKEDAGIVPLGIPMLAGPGAISSVMVLVGE
                     APDHWQMMAILGSIAITAFVSYLVLSGADRIRRFLGETGIRILVRIMGLLLVALAMQF
                     FVNGLTDLGLIQRH"
     gene            6853..7248
                     /locus_tag="VHE33_00090"
     CDS             6853..7248
                     /locus_tag="VHE33_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95872.1"
                     /translation="MMTRSDKPPSQKLAEVSILLASDSERHGNTMHRALEQHGLPVEY
                     AGDYAQLESALHQRAFDVVLLEVTSEDAVEPAVAAALRLKRRDARQFVGYLADPNLET
                     SGLAGDAVFSRNAARLPEALRRFVAEGGN"
     gene            7346..>7804
                     /locus_tag="VHE33_00095"
     CDS             7346..>7804
                     /locus_tag="VHE33_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95873.1"
                     /translation="MRKLFTATWMTAAVCALLWAKAPAWAQGAAAGPFHIQQEWKIGG
                     DGFWDYLAVDPQSRLLYITHGNHVAVVDTHAGRQVADITGLHGTHGVVFSTDGVHGYI
                     TDGGANQVAEFNRKTNTIEKTIPAGTGPDGAVFDPYTKTVWAFNGRSHDAT"
BASE COUNT         1581 a         2371 c         2165 g         1687 t
ORIGIN      
        1 tgcaaatcac ccgacgctag ccctaaagct atttcggaga gaacgagcta tcacggaatt
       61 tgattagcct ttcaccccta ccctcagctc atccgagctt ttttcaacaa acaccggttc
      121 ggtcctccaa taggttttac cctaccttca acctggccaa gggtagatca tcccgcttcg
      181 cgtctattcc tgccaactga acgccctgtt cagactcgct ttcgctgcgg ctcgcttacg
      241 cttaacctcg ctgacaagaa taactagccg gctcattatg caataggcac gcggtcaggc
      301 atttccttgc ggacatagcc ctcccactgc ttgtaggcac acggtttcag gtacttttca
      361 ctcccctaac aggggtgctt ttcgcctttc cctcacggta ctggttcact atcggtcaga
      421 atcgagtatt tagccttgcg ggatggtccc cgcgggttca gacagagttt cacgtgctcc
      481 gccttactca ggtatccgct tcgagtccgg attgttttcg cctacgcgcc tgtcacgctc
      541 tgtggatccc ctttccagag gattcggcta acaaccggat tggtaactct actgttgcgg
      601 accctacaac ccctgggaca ccgaaatgtt ccaggtttgg gctgttccga tttcgctcgc
      661 cactactttc ggaatcgagg tttctttatt ttcctccagg tactgagatg gttcacttcc
      721 ctgggttcgc tcgtacccgc ctatggattc agcgggccgt acatgaggtt tgctcatgtg
      781 ggtttcccca ttcggacatc ttcggatata gcgcctgtgt gcggctcccc gaagcttttc
      841 gcagcttgcc acgtccttca tcgcctgatt ctgccaaggc atccaccgtg cgcccttagt
      901 agcttaacca tagaattaac tccacgcagg agttaaaact ctgcattcgc ctgtgcactg
      961 tctcacgaca gtgcctagat atctaaagac actcaacttg aacaacttgc cttgcttcac
     1021 gcattcgcgt ttccgcgacc gcgcgcgcag ggcttattgc tcagccttgt atatttaccc
     1081 aatcgatttc gttgtcaaac atcgcttgcg cttgcctcgc ggctcacgct ccgccctgag
     1141 gcggcgagag aaaccttacg gcttctgctc aaaggtcgaa gtccagcgcg tgggcgccga
     1201 cctttgagca gcttctcgaa cagggaagtg gtggagctga ccgggatcga accggtgacc
     1261 ccctgcttgc aaagcaggtg ctctcccagc tgagctacag ccccatttaa ggatggtggg
     1321 cctgggtaga ttcgaactac cgacctcacc cttatcaggg gtgcgctcta accaactgag
     1381 ctacaggccc ggcttgcgcc ttattcctgt ctgtgtcagg tcccttcgag aaaaggtttc
     1441 gaggaccagg gtgaacatgc tgaccagcta cgccggtcat gaccatcgtg ttgttccgga
     1501 aagacagaga aggtctgatt caggctccct tcggttcggt caggaaccgg actgaagggg
     1561 tacatggcaa catcccgcta actcaaatcc gaagatctgt ccgcgatacg cggagcgtta
     1621 gtacgagtgc catgacgttc tctcttagaa aggaggtgat ccagccgcag gttctcctac
     1681 ggctaccttg ttacgacttc accccaatca tgaattacac cttgggcggc tgcttccttg
     1741 cggttagcgc accgacttct agtgcaaccc actttcgtga tgtgacgggc ggtgtgtaca
     1801 aggcccggga acgtattcac cgcggcgttc tgatccgcga ttactagcga ttccagcttc
     1861 acgcagtcga gttgcagact gcgatccgaa ctgaggccgg ctttttccga ttagctcccc
     1921 ctcgcgggtt tgcaacggtt tgtaccggcc attgtagcac gtgtgtagcc ctgggcataa
     1981 aggccatgag gacttgacgt catccccacc ttcctccccg ttatccgagg cggtttcgcc
     2041 agagtgctca actaaatggt agcaactgga gataagggtt gcgctcgttg cgggacttaa
     2101 cccaacatct cacgacacga gctgacgaca gccatgcagc acctatattg cagccccttg
     2161 cggggaaggg atatttctac cccggtccac aacatttcga gtccaggtaa ggttcttcgc
     2221 gttgcgtcga attaaaccac atgctccacc gcttgtgcgg gcccccgtca attcctttga
     2281 gtttcagcct tgcgaccgta ctccccaggc ggattgctta tcgcgttagc tgcggcacgg
     2341 cgggattggg tacccgtcac accaagcaat catggtttag ggctgggact accagggtat
     2401 ctaatcctgt ttgctccccc agctttcgtg catcagcgtc agttatggtc cagtgagccg
     2461 ctttcgccac aggtgttcct cccgatatct acgcatttca ccgctacacc gggaattcca
     2521 ctcacctctc ccacactcaa gccccgtagt ttcctgtgca ggcttcgagt tgagcccgaa
     2581 gatttcacac aagacttgcg gaaccgccta cgcacccttt acgcccaata attccgaaca
     2641 acgctcgccc ccctcgtatt accgcggctg ctggcacgaa gttagccggg gcttcttcat
     2701 ccggtaccgt cattatcgtc ccggtcgaaa ggagtttacg tcccaaggga cttcatcctc
     2761 cacgcggcgt tgctgcgtca gggtttcccc cattgcgcaa aattccccac tgctgcctcc
     2821 cgtaggagtc tggaccgtgt ttcagttcca gtgtgtccgt gcgccctctc aggccggata
     2881 cagatcatcg tcttggtggg ccattacccc gccaactaac taatctgccg cgaccccctc
     2941 cttaagcgaa ttgctccttt gacccgtagg tgttatgcgg tattagcccg agtttcccca
     3001 ggttattccc cactcaaggg taggttagtc acgtgttact cacccgtgcg ccgctttact
     3061 catggattgc tccactttct cgctcgactt gcatgtgtta ggcacgccgc cagcgttgat
     3121 tctgagccag gatcaaactc tcgtttgaaa cctgagattc cttaacccga aggacggagg
     3181 tcctcgggtg gaatcggctt cgcaagcgct cgaaaacgct gcgaagcact acattgtgag
     3241 aatgatcaaa cctgatcttt tcgtattctc acgactggca tgttcatcct gattgtcaaa
     3301 gatcctgacg gctacccgcc tgagcggacc gctttggatc gagagcgcga ggggttttga
     3361 ggcccgtttg cgcgtcctcg aacttgttgc gcctgatttt tgcgcgaact ttccgagatt
     3421 atcgaatctc gtcggtgtct gtcaaggaat cgccttaact tctttgatcc ctgcaccgcg
     3481 tcgcacagac agaaactccc cgtttccgga taggtcctgc agacgccaaa cttagaaaga
     3541 cctgaccgcg agcagtactc gcgggggcgg ctgaccgccc gatgggattt tgcagggcaa
     3601 agttcacccg gcagccccgt ttgcaatgga ttgttctgtt ttcttaccgt gcgcccgagg
     3661 cgcaacgact gtcgaacaga tcccgcttcg ccttcaacac aaccggggaa gaatcaccct
     3721 tcaacgaagc cttgccactg ctaccaatcc tcttcacttt accatgccgc gctgctcttg
     3781 tgcaaatcgg ccagggtgaa gcgacttgtt gatagtacgt gatcgacgca aatgcggcaa
     3841 gttgaggaaa acagccgaaa tctgtgggaa acgccctgcc ggagccagcg ccaaagccac
     3901 ccacggccgg agcgagggct cagagcgaag cctgaagcag agcgatggaa tgcgcaggct
     3961 gctggaaaag accgttttca aatgtcacgc catgggtttg cggtaccacg cgttccggat
     4021 gagcgacgtc gttgtaatcg tctggtgagc cccctgcgag aatcgtcacg gtggcctccc
     4081 ggaggttgat tcccgagcca aaatccatct tccagcgtgc aacctggtcc gggtgccgat
     4141 tgaccagcgc gacggcgatc tgccggcgat cgtcgctgca ggtggccagc ccgtccagag
     4201 ccgccaccga actctgatcg acgcgcagcg ggccgcagcc gacctggctg gaaacgacat
     4261 tgcgctggag ccgatccgaa tacatccgaa ggacatagaa ggtggtgcgt ttaacgattc
     4321 cgcggggatg aacgaataac ggcccgcgcg cgttcacgac cggcgccatg cacgccatct
     4381 ggacatgttt cgcgttgcgc agacaggcat tcagaaacga cgcggaaaac agggcgtccg
     4441 ccatggtgta ggtttcgttg cgatcgttct catcgcgctc gcggatcatt tcgacctgat
     4501 cggccccgcc cccgggaaag ccgggatggt gccacccgcg cagattccat tcatcgaaag
     4561 caatcccgat tcgatccccg aaccccacct gctcgaggag ttcaaccgcc tggcgaatct
     4621 gctgttcggg ctgctcgcat cgggccatgc aatgcaggta gtcgctgggt ttggtgccgc
     4681 gttgccacag cgggtcccag tagccatgga tcgagatata gtccagatat tttcccgcgg
     4741 ctttcagcat gggcggggtc cagttgccgt tgacggtggc cgcagccagg atcttcagcc
     4801 cgctgtcgac ggcacgcatc atcttcgccg attcacggac caggggagcc cactcctcga
     4861 ccgtcttcgc gccaatctcc ccgcccaggt aattttcgtt gccgatgctc cagtagcgaa
     4921 cgtcaaaggg ttcggcaaag ccgttcgccc tgcgcatgcg ggcataacgc ccctggtcct
     4981 tcaggttgca gtattcaacc cagtcgctca tttcctcctg ggtgccggtt cccgcattcg
     5041 tgcaaatgta tggggcggcg ccaatcctgc ggcaccattc gacaaactcc gccgtaccga
     5101 aggtgttcgg atcctcgacg ccccaggcct tgtccagcgc cggctggcgg ttgtctccca
     5161 caccgtcgcg ccaatgatag gccgaggcaa agcagccgcc gggccagcgg actacgggaa
     5221 tcttcagctc gcgcagggcc tcgatgacgt cgatgcggaa cccacggtgg tccgacagcg
     5281 gcgatccggg atcgaagacg ccgccgtaaa cctggcgatg aaaatgttcg aggaattgcc
     5341 caaaaagcat gcggttgaac ggagtgagag gagccgaaac atcgatcctc aactcacccg
     5401 caaccgctcc cggagaatcg aaaccggcaa ggttccgcgc cagcaggctc gagtcaagaa
     5461 ctgcggcgcc tgcgccctgg gcaaactgcc gccgagtggg aagaaaccca gcatccttca
     5521 tagacgagac ggtccttgtc gaagctggct tgaaagtgcg tcccgattct actgcatgaa
     5581 acggccagtc ttgcgtgtcc ttcgaagaac ccgaagaagc cgcaacgatg cacgctagtg
     5641 tggtgtttct gaagttcctt tcccttgtca tcccgaccgg accctgagcg tagcgaaggg
     5701 gaagcggagg gacatgcttc tcttccgcat aaagaagttt cggaagcacc acgctagtgc
     5761 cgctggatga ggcccaggtc cgtcagcccg ttcacgaaga actgcatggc cagcgcgacg
     5821 agtaacaggc ccatgattct caccaggata cgaataccgg tttcccccag gaatcgccgg
     5881 atgcggtccg cgccgctcag cacaagatag ctgacgaagg cggtgatcgc gatcgagcca
     5941 aggatcgcca tcatttgcca gtggtcgggc gcttctccta ccagaaccat cacgctcgaa
     6001 atcgcgccgg gaccggccag catcgggatc cccagcggca cgatgcccgc atcctccttc
     6061 gccgcggctt cctgcgtttc ctcctgcgat tcctgcgtgg gggagcggcg agcctgcatc
     6121 atgtcgatgc cgatcagcag aaggatcagt ccgcccgcga tttcaaaagc cgggagcttg
     6181 atgccaaaga gggaaaagat gtactggccc gcaagggcaa acgtgatcag gacgatgaag
     6241 caggtcagcg ccgccttcca tgccatgcgg cggcggcgcg ccggctcaac gtcggaagtg
     6301 atggcgataa acgaggggat cgctgcgaaa ggatcgacga gaaagaagat ggagctcagc
     6361 gccagcagag aaaacttcac atagctcgag ttcagcgcct gctcggcggc ggccgtcggg
     6421 gacaccatga cggcaaagtg ttgaatcaac atgggctgga gatacatttt tcaggatatc
     6481 cgactcattg gatgctcggg ggtgtccgct cggtccaggc cgcctcgtcg agccgagcag
     6541 ttttgggaat cgaccgagcc gaagttgctc gacccgattt ggttgcctgt ggatatcccg
     6601 cgattaagcc cttcagcagc aatgagctgc acgattctcg gcaggtttgg acccgctgtt
     6661 gcatctctcc cggctgcccg gatgaaggtc gcacgcagga ccgaattccg cgcacggttc
     6721 tcggagtttg cactctggaa cgattttcaa agccgggttc aaagcatcac tccccggccc
     6781 agggagatgc gcggtccccg aggccagaca actcaagtgg tccatcacgt aagaggagaa
     6841 tgcatgcccg ttatgatgac ccgatcagac aagcctccaa gtcagaagct cgccgaggtt
     6901 tcgattcttt tggcgagcga ttccgaacgg cacggaaaca caatgcatcg agccctcgag
     6961 caacacgggt tgccggttga atacgcggga gattacgcgc agctggagtc agccctccat
     7021 cagcgtgcct tcgatgtcgt gctgctggaa gtgaccagcg aggatgccgt cgagcccgcg
     7081 gtggcggccg cccttcgctt aaagcgcagg gatgcgcgcc agttcgtcgg ctatcttgcc
     7141 gatcccaatc tggaaaccag cggactggcc ggcgatgcgg tgttttcgcg caatgcggca
     7201 aggctccccg aggcgctgcg gcgcttcgtg gccgagggtg ggaactaatt cctttcaggg
     7261 aagccctgcc tgggttttct tcagccaaaa ttcagcaacg cggagtactc tggctgcgct
     7321 ttgttcgcga gggggcgagc cagccatgag aaaactcttc accgcaacat ggatgacggc
     7381 ggctgtttgc gccttgttat gggccaaggc gcccgcgtgg gcgcagggcg cggcggccgg
     7441 gccgttccac atccagcagg agtggaagat cggcggcgat ggcttctggg actacctcgc
     7501 cgtcgatccg caatcgcgat tgctctatat tacgcatggc aatcacgtcg ccgtcgtgga
     7561 cacgcacgcc ggcaggcagg ttgctgacat caccggtctg cacggaaccc acggagtggt
     7621 gttttccact gacggcgtcc acgggtatat caccgatggc ggtgcgaatc aggtggccga
     7681 gttcaaccgg aagacgaaca cgatcgagaa gaccattccg gcgggcacag gtcctgacgg
     7741 cgccgtgttc gacccgtata cgaagacggt gtgggcgttc aacgggcgaa gccacgatgc
     7801 gacc
//
LOCUS       DASZYN010000005         1565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_424, whole genome shotgun sequence.
ACCESSION   DASZYN010000005 DASZYN010000000
VERSION     DASZYN010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VHE33_00100"
     CDS             <1..984
                     /locus_tag="VHE33_00100"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HVT95874.1"
                     /translation="PMAALAGAGGLGLKSPNDMYVAMLKSQTVEDGMVQRFHLESDYK
                     EKRLSDARKTFERHATVDASGKDGLIHISVEARNPDRAVELANGYIDQFRKLSQNLAI
                     TEAQQRVLFFQRQLEQAKDSLANAEVGLQKTEQKTGLIELDSQARALIASAASLRAQI
                     AAKEVQIQAMQTFASGGNAQLLQAEQELDGMRAQLAKLGGTEDNPNTLIMPKGKLTEA
                     GLDYVRKLRDVKYYETMFDILARQFEIAKLDEAKEGSLIQVVDPPVRPDRKSFPKRGL
                     IVAIATAAGFLIGILAALVQAGWSRLKEDPEARGKLSLLRHALRSKSSSIP"
     gene            complement(1017..>1565)
                     /locus_tag="VHE33_00105"
     CDS             complement(1017..>1565)
                     /locus_tag="VHE33_00105"
                     /inference="COORDINATES: protein motif:HMM:NF025668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HVT95875.1"
                     /translation="ILTGKTNPSGRLPVTFYASDSQLPPFDDYNMKNRTYRYFTGEPL
                     YGFGYGLSYSTYSFSDLKLSSSSLTAGDPLTAEVKVTNTGNRAGDEVAELYLIPPAGK
                     GYPLRSLQGFERVHLAAHASAVVRFRLNDRDLSEVDSGGHRAMRAGEYQIWVGGSSPE
                     SATTPGGILGRLTITGSKDLPE"
BASE COUNT          346 a          429 c          513 g          277 t
ORIGIN      
        1 cccatggcgg cactggcggg ggcgggtggc ctcggcctga aaagcccgaa cgatatgtat
       61 gttgccatgc tgaagagcca gacggtcgaa gacggcatgg tgcagcgatt ccatctcgaa
      121 tccgactaca aagagaaacg tctctccgac gcgcgcaaga ctttcgaaag gcacgcgaca
      181 gtggacgcgt cgggcaagga tggtctgatt catatctcgg tggaagcgcg gaatccggat
      241 cgcgctgttg agctggcgaa tggttatatc gaccagttcc ggaagctctc ccagaatctg
      301 gcgattacag aagcacaaca gcgggttctg ttcttccagc gacagctgga gcaggccaaa
      361 gacagtctgg cgaacgcgga agtgggtctg cagaagaccg agcagaagac aggactgatc
      421 gagctggaca gccaggcacg cgccctgatc gcttctgcgg cgagcctgag agcgcaaatc
      481 gcggcgaaag aagtgcagat ccaggcgatg cagacgtttg catcgggagg gaatgcgcag
      541 ctgctccagg cggaacagga actggatggc atgcgagctc agctggccaa gctggggggc
      601 acggaagata accccaacac tttgatcatg ccgaagggca aactgaccga agcgggcctg
      661 gactacgtgc ggaagctgcg cgatgtgaag tactacgaga cgatgttcga cattctcgcc
      721 cgccagttcg agattgcaaa actggatgaa gccaaggaag gctctctgat tcaggtcgtg
      781 gatccgcccg tccggccgga tcgcaaatcg tttccgaaac gcggcctgat cgtcgcgatc
      841 gcgactgcgg ccggcttcct catcggcatt ctggcggcgc ttgtgcaggc cggctggagc
      901 cggctgaaag aagacccgga ggcgagaggc aagctaagtc tgctgcgcca cgcgttgcgg
      961 agcaagagct cgtccatccc gtaattttgg ccgcggccgt gcaggccgag gtcgcgctat
     1021 tcgggcagat ccttcgatcc cgtgatcgtc agcctgccca aaatgccacc aggcgttgtt
     1081 gcggattcag gcgaagagcc cccaacccat atctgatatt caccggcgcg catggcacga
     1141 tggccgccgc tgtccacctc gctgagatcg cggtcattca gcctgaaccg cacaacggcg
     1201 ctcgcatggg cagccagatg gacgcgctca aagccctgga ggctgcgcag aggatagccc
     1261 ttgcctgcgg gcgggatcag gtagagttcg gcgacttcgt ccccggcacg attcccggtg
     1321 ttggtgacct tgacttcggc ggtgagcgga tcgcccgcgg tgagcgagct tgaggatagc
     1381 ttgagatcgg aaaagctgta agtcgaataa ctcaggccat agccaaaacc ataaaggggc
     1441 tcgccggtga agtatcgata ggtgcggttc ttcatgttgt agtcgtcgaa aggcgggagc
     1501 tgcgaatccg aggcgtagaa ggtgacaggc aagcggcccg aaggattcgt ctttccggtg
     1561 aggat
//
LOCUS       DASZYN010000006         3621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_771091, whole genome shotgun sequence.
ACCESSION   DASZYN010000006 DASZYN010000000
VERSION     DASZYN010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771091"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VHE33_00110"
     CDS             complement(<1..964)
                     /locus_tag="VHE33_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HVT95876.1"
                     /translation="MAQAPPIPVPIASEADETAQAQALARRYRSEFIDLRNFRIRHEV
                     LRSVPVDMMFRYNFVPLEQEEDRLIIAVSDPSKLMMLDEISGLLGQRIVTRVATLSQI
                     TDLLKKTEQSQRVLDEASEGMTFDVLSAGEDSDENISIEKLTSSEDISPIIRLVDTTI
                     FTALERRASDIHIETYDDSLHVKYRIDGVLQPAMAPIAREHHLTILSRIKVMSELDIA
                     ERRVPQDGRFRVRYKGRLIDFRVSIMPTVHGENAVLRVLDKESMSEKFRQLTLDVVGF
                     HEDDLRRFRRYIREPYGMVLVTGPTGSGKTTTLYAALNEIKSDED"
     gene            complement(1087..2283)
                     /locus_tag="VHE33_00115"
     CDS             complement(1087..2283)
                     /locus_tag="VHE33_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVT95877.1"
                     /translation="MAEFVIRLADERGHVQEQIQSAATAEELRTRFSQAGYFVYSVKP
                     RGLTGSARKTKLETFLIFNQQFLTLIRAGMPILGSLEMLAKSQKNANFSGQLQNVAAR
                     VRTGEPISSAFEAQGAFPLMYTTTLLAGERSGNLEEVLGRYLSFQRVALAFRKKLIAS
                     LIYPLMLISLVICLFIFLITFVVPQFAKLYDQIGTKLPSITLLMLSLGTGAQKYLLYI
                     LPALALIGYLLWRWSRSEGGADRIDRIRIRLPLFGKIWLKYQVAMFARTLSTLLSGGI
                     PLVPSLETAAKSITSRRIAKSVFSAVTSVREGKGLARSLEATKVFPELSTEMIEVGES
                     TGALPGMLNSVAEFFEEDVQTALTAALALIEPAILIVMGVVVVIILIALYLPIFSMSN
                     AQNFRS"
     gene            complement(2466..3575)
                     /locus_tag="VHE33_00120"
     CDS             complement(2466..3575)
                     /locus_tag="VHE33_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95878.1"
                     /translation="MLRRASCLGMLLALGGFAWGQGLNSQSGAWVLERSGTTVSLRGV
                     QSLGGGVAWASGAEGTVLRTEDGGYEWQSCAMPPDADKLDFRGLWAWDANTAVVMSSG
                     TGEQSRVYRTTDGCSHWTLVLTDPDKEGFFDSIVFSSRQVGYLLGDPVDGAFPLFRTG
                     DGGATWTKIQSPGLTTDGLSIAAFAASNSSLAADHRVWFVTGGQGGAFLYEGMSDCPG
                     EGDGEAAPDCSDHWTFHRQPLPMAGGSASAGAFSVRAAWWNGRVRAAVAVGGDYAKPD
                     VSAGTSAWTPDGKTWKASEKAPGGYRSAVNWDANLNAWIAVGPTGSDVSWDGGRHWKP
                     LDGVETGANWNALSLPFAVGSDGKIGKINLGELHK"
BASE COUNT          698 a         1313 c          924 g          686 t
ORIGIN      
        1 tgtcttcatc gctcttgatc tcgttcagcg cggcgtaaag tgtcgtcgtt tttcccgatc
       61 cggttgggcc cgttaccaga accatcccgt aaggctcgcg gatgtaccgg cgaaatcgcc
      121 tcagatcgtc ttcgtggaag ccgaccacat ccagcgtcaa ctgacgaaac ttctcgctca
      181 tcgattcctt gtccagcacg cgaagcacgg cgttttcgcc atgcaccgtc ggcataatgg
      241 agacgcggaa gtcgatcaga cgacccttgt atcgcacgcg gaagcgtcca tcctgcggca
      301 cgcgacgctc agcgatatcc agttcgctca tgaccttgat acgcgacaga atggtcaggt
      361 gatgctcacg cgcaatggga gccatggcag gctgcagcac accgtcgatg cggtacttca
      421 catggagcga atcgtcgtag gtctcaatgt gaatgtccga cgcccgccgt tccagcgccg
      481 tgaagatcgt cgtatccacc aggcgaatga tcgggctgat gtcctctgaa gaggtcagct
      541 tctcaatcga gatgttctcg tcggaatcct cgcctgccga cagcacatcg aaggtcattc
      601 cctccgatgc ttcatccagc acgcgctggc tctgctcggt cttcttcagc agatcggtga
      661 tctgcgacag cgtcgccacc cgcgtgacga tgcgctgccc cagcaggccg ctgatctcgt
      721 ccagcatcat cagcttgctt ggatcgctca ccgcgatgat cagcctgtcc tcttcctgct
      781 ccagcggcac gaagttgtag cggaacatca tgtccaccgg cacgctgcgc agcacctcat
      841 gccggatgcg gaagttgcgc aggtcgatga actccgagcg atagcgacgc gccagcgcct
      901 gcgcctgggc cgtttcgtcc gcctcggacg caatgggaac cggaatgggg ggagcctgag
      961 ccatggtcgg gatacctctg tgaaacacct gcctaaacga tcacttgcaa cgcgctaact
     1021 cacaactctc aactcacgcc tcttcactct tcactctcaa ctcttcactc tcaactcaca
     1081 actcactcag cttctgaaat tctgcgcgtt gctcatcgag aaaatcggca gatacagcgc
     1141 gatcagaata atcaccacaa ccacgcccat cacgatcaga atcgccggct cgatcagggc
     1201 cagcgccgcg gtcagggccg tctgcacgtc ctcttcaaaa aattcagcca ccgaattcag
     1261 cattcccggc agtgcccctg tcgattcgcc tacctcgatc atctccgtgg acagctccgg
     1321 gaaaaccttc gttgcttcca ggctcctcgc cagccctttg ccttcgcgta ccgacgtcac
     1381 cgccgaaaac accgatttcg caatccgccg gctcgtgatg gacttcgccg ccgtctccag
     1441 cgacggcacc agtgggatgc ctccactgag cagcgtggag agcgtgcgcg cgaacatcgc
     1501 cacctggtac ttcagccaga tctttccaaa cagcggcagc cgaatccgga tgcggtcgat
     1561 gcggtccgcg cccccttcgc tgcggctcca ccgccacagc aaatagccga tcagcgccag
     1621 agccggcaga atgtacagca gatacttctg cgcgcccgtc cccagcgaca gcatcagcag
     1681 cgtgatcgag ggcagcttcg tcccgatctg gtcgtacaac ttcgcaaact gcggcaccac
     1741 aaacgtgatc aggaagatga acaggcaaat caccagactg atcagcatca gcggatagat
     1801 cagcgacgcg atcagcttct tgcgaaacgc cagcgccacc cgctggaacg acaaataccg
     1861 acccagcacc tcttccagat tccccgaccg ctcccccgcc agcagcgtcg tcgtatacat
     1921 cagcggaaac gcgccctgcg cctcgaatgc cgacgagatc ggctcccccg ttcgcacgcg
     1981 cgcagccacg ttctgcaatt gtcccgagaa attcgcgttc ttctggctct tcgccagcat
     2041 ctccagcgat cccagaatgg gcatgcccgc ccgaatcagc gtcaaaaatt gttgattgaa
     2101 gatcaggaat gtctcgagct tcgtcttcct cgcgctgccc gtcagccccc gcggcttcac
     2161 cgagtagaca aagtaccccg cctgggagaa ccgcgtgcgc agctcttccg cagtcgctgc
     2221 cgactgaatc tgctcctgca cgtggccgcg ctcatcggcc agcctgatca cgaattctgc
     2281 catagttttt ggctatacga ttcttcggct gcctgccttt tcgccttcgg ccgccacacc
     2341 tcttccggcg ccggaatacg cccgcgccgg ccctggagat gcctccttcc agcgtagcag
     2401 ttcggcgctc cacacgcctc tggcgcaggg tttcgtggac tagacgtttc caggcgccaa
     2461 aatgctcact tgtgcagctc ccccaggttg attttcccga ttttcccgtc cgatcccacc
     2521 gcaaatggca ggctcagagc attccagttc gcgccggttt cgacgccatc cagcggcttc
     2581 cagtgccggc cgccgtccca gctcacatcc gaccccgtgg gccccaccgc aatccatgca
     2641 ttcaggttcg cgtcccagtt cacggccgac cggtacccgc caggcgcctt ttccgaggct
     2701 ttccaagtct ttccgtcagg cgtccacgcc gaggtccctg cgctgacgtc cggtttcgca
     2761 tagtctccgc ccaccgccac cgccgccctc acccgcccgt tccaccaggc ggctcgtacc
     2821 gaaaatgccc ccgctgacgc gctgccgccc gccatgggca gcggctgccg atgaaacgtc
     2881 cagtgatccg agcagtcggg agcggcctcc ccgtcaccct cacctgggca atcgctcatt
     2941 ccctcgtaaa gaaacgcccc gccctgtccg cccgtcacga accacacccg atgatcagcc
     3001 gccagcgagg aattgctggc agcgaacgcg gcgatgctca gtccatccgt cgttagtcca
     3061 ggagactgaa tcttcgtcca cgtcgctccg ccgtccccgg tccggaacag tggaaacgcc
     3121 ccgtccaccg gatcgcccag caggtaccca acctgccggc tgctgaagac gatcgaatcg
     3181 aaaaaccctt ccttgtccgg atcggtcagc accagcgtcc agtgcgagca tccgtccgtc
     3241 gtgcgataca cccgcgactg ctccccggtc ccgctcgaca tcaccacagc cgtgttcgcg
     3301 tcccacgccc acaggccccg gaaatccagc ttgtctgcat ccggaggcat cgcgcagctc
     3361 tgccactcat aaccgccgtc ttcggtgcgc agaaccgtgc cctcggcgcc gctcgcccag
     3421 gccactcccc cgcccagcga ctgcacaccg cgcaggctca ccgtggttcc gctcctttcg
     3481 agaacccatg cgcccgattg actgttcaga ccctgccccc aggcaaagcc gccgagcgcc
     3541 agcagcattc ccaggcagct tgcacgcctc agcatggcgg catgctaccg gcccagggca
     3601 cccggaaaac atcccacgga a
//
LOCUS       DASZYN010000007        13001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1028358, whole genome shotgun sequence.
ACCESSION   DASZYN010000007 DASZYN010000000
VERSION     DASZYN010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 13001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13001
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028358"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1402
                     /locus_tag="VHE33_00125"
     CDS             <1..1402
                     /locus_tag="VHE33_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVT95879.1"
                     /translation="VAPLVSHALGAMLLSGARESHLDTSLDVRVFAFAALMAVTATLV
                     IGLVPALRATSRDLNEQIKSGQHSTQKQERHRWLPRALMSVEVALALVLVVGAGLLAS
                     TLVRLYRSGEGFDPHGVQNVAFSMEKSGMNHASLMAFYQQIGEGLRRVPGVTAVSFAR
                     IVPLTGYVWDEDMSASGGKPTDTYFNAVGPGYFSAMRIPLREGRDFGWNETTAEGGKI
                     VLNAQAAKLLFPHGGALGRIVNRSRGPKESPLPYEVVGVVGDAKYEDLRSQAPPAAYT
                     AMTQEEGKDSPSSYYAVVRTSGDAGSVASAARKLAQHLAPGIPAPVTTSMMTTVDDSL
                     SAERMMALLSVFFAACALLVTAIGLYGTLAYATARRTSEIGIRMALGARRSQVVGLVC
                     SQNALVALVGTAVGLIAALLASKALASFLYGTGAQDPWVLIGSVAALAVIASAASLLP
                     ALRAARINPVVAIRCE"
     gene            complement(1413..2924)
                     /locus_tag="VHE33_00130"
     CDS             complement(1413..2924)
                     /locus_tag="VHE33_00130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HVT95880.1"
                     /translation="MNAPRTRLSDTTLLLFSGLSIALLHIAVNGRYGFHRDELLTYSN
                     ARHLAWGYVVYPPLTPFLGRLELLLFGSSLRGFRFLPAVAQGLAMILTGLSARAMGGL
                     RWAQLVAAAAVAISGFSLFNGGFLSYSTFDYLWWVLAGYCITRLLASDDPRWFLAVGA
                     AIGLGLMTRYTIGVLIVGILVGLLLTPYRRCYKSLWFWAGAAVAVFVCLPNFLWQMHH
                     HFIWLQWFRSIHARDIHQGSTDFFLLNQLWKTTNPVTVPFWLGGLWYVFRKPGGKRWR
                     LLGWMYVIPALTLFVVHGRDYYLSPAYPMLFAAGAVQAEHWLATLSPGAAGWVRSTTW
                     QSFVISGLSITALAVPVAPVGTAWWHFADACTGGNFNSQIGWPEMVAQVAHIRDGLPA
                     EDQSRLAIATGDDGETGAIDLYGPAYHLPPAISGMNSSWARGYGDPPPQTLIVIDGDP
                     DFLRQHFESCFVAGRVTNSVGIRTSSIGKPDLYVCRGLREPWQEFWAHLQYYG"
     gene            3073..3978
                     /locus_tag="VHE33_00135"
     CDS             3073..3978
                     /locus_tag="VHE33_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVT95881.1"
                     /translation="MEALVELDHVSKSYEEKAAVHDLSLKIEAGTMFGLLGPNGAGKT
                     STIRMMIGITMPDAGKVSLFGKPFGNHALKRVGYLPEERGLYKKMKVIDQLVFLAELR
                     GLGALEAKRRAKQWCEKLQIADSIEKRTEELSKGMQQKIQFISTLLHDPELIIMDEPF
                     SGLDPVNAVLLQDTLVELKKNGNAVLFSTHRMDQVEKLCDSICLINRGEAVLSGGMRE
                     VKSRYERNRVIVQFEGNDEFLGHPAIADAKSYVGHAEIKLKPHADAQDLLRTAAAMAT
                     ITKFELVEPSLEEIFIETVGGRADA"
     gene            3971..5236
                     /locus_tag="VHE33_00140"
     CDS             3971..5236
                     /locus_tag="VHE33_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT95882.1"
                     /translation="MRDGLLRNTLLIAKREYLERVRSRAFRIITLLVPIGMAGMIFLV
                     SMSGKKLEGLQNLAILADDPGLAQQVKAALEDGAFKPNRVEVYAPATDADLSRVNGEI
                     ATHQIQGYLKLKTVEGEKEPDATWISGSSINLVGKEQTESAVRSALIRRELLARGMRE
                     DQVHDLMQETKLETKQLKNGTAVSSDSDKSFAGADALIILLYASVLIYGINIARSVVE
                     EKTSRIFEVLLSTASADSLMLGKLFGVGAVGLTQMGIWVGLVVLYAGSAMAASQGIHG
                     LNSLGLTRVELMFFPFFFLGGFLLYSALAAALGSSVSGDQEIQQFSFILISPLVASVV
                     MLPYVLGNPNSTASIVLSLLPPFTPIIMYMRICAQMPPAWQVWLSVALLAASVWAMIW
                     LAARIYRVGVLMYGKRATLPEMIRWLKYS"
     gene            complement(5263..5592)
                     /gene="yidD"
                     /locus_tag="VHE33_00145"
     CDS             complement(5263..5592)
                     /gene="yidD"
                     /locus_tag="VHE33_00145"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HVT95883.1"
                     /translation="MPPPGKALRHPATWLAVFLVFAALVAADSFRTPSRQVSVRVFTA
                     AVAGYHHYLHPITGRWIHCRYQPTCSRYSVEAVRKYGIAKGLALSFRRIRSCRQSVPM
                     GTRDPVP"
     gene            complement(5603..5971)
                     /locus_tag="VHE33_00150"
     CDS             complement(5603..5971)
                     /locus_tag="VHE33_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HVT95884.1"
                     /translation="MRKPGLSLLCLGIALFTGSALAQNDDASAAAGAAGCGICGSFFL
                     LFFLAIIALNIAILVWVARDAKARGLDNGVLWMLLVLFTGVVGLIVYLLARPQGNLVV
                     CPNCGNKRMQAAVKCPNCGA"
     gene            complement(6098..7474)
                     /locus_tag="VHE33_00155"
     CDS             complement(6098..7474)
                     /locus_tag="VHE33_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVT95885.1"
                     /translation="MELVEAERPSVREIPNGAKRLAEICPTPGVTMTGSSIPAVLMLR
                     CAAVSHHSVRAAARHAGLRLTLEFVENRREFLEELHRGSTDLIIAGTEGLTDLDVREV
                     IDRARSADIPIPVLLAGGRTGEAGAVRMLRDGATEIVQSGELERLPSAIARALKVRQS
                     AIVQSHAQHELDRSAVMPRENQKLITIGRLAASIAHEINNPLESVMNLLFLLGEEKSL
                     SPTVTSYLALAQRELDRVAQISRQTLKFSRETSGPVPVHIEELLEEVLSLYGRRMMEK
                     NLRVERQYDCGEEAIVYPGEMRQVLSNLVTNAIEASSLRGRLRLRVRSTRSWSDPQVR
                     GIRVSVADTGSGIPPEVQRRLGEPFFTTKGQRGTGLGLWVTRSIIQRYGGEMQLRSSV
                     HPDRHGTVFTIFLPTNLRPRAVDRPPHGAGENGGSGSVTPFPAPGSSGAAADISRGGE
                     SARRSRGA"
     gene            7781..9265
                     /locus_tag="VHE33_00160"
     CDS             7781..9265
                     /locus_tag="VHE33_00160"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HVT95886.1"
                     /translation="MKTRVAGIIVGGALLLGAAAGAQSNPGNSAANPYMGSVQAVAFS
                     PEPKALSLDDAIKLGIQNNLALTLAREQQKAADATKLQLVTVLLPKITLHGETGVHQY
                     NLEALGFHPASLSDFASHLPPGASGSFPLVTKVDTTIGEVNFSQALFNWAGYDVWRAS
                     QAYTKAAYYNAQSSRGLVVLNVGNTYLQALAAKARLEYAQALVKTDETAFNQAHEEHL
                     AGVVANLDELRARVQLQSQQQAVIAAQNNFDKAKIALNREIGLRPEQAIILTESSPYA
                     ELHPMDLVDARIEAYANRQDYLGLKQELIVADRERAATRHERFPTLTFGGNWGVTGVT
                     GGVYHETFAAVGTLSIPLFKEAEFRGDRDVAEAQLEEIRSRLEDLKEKIDQQLRDSLL
                     DLQTATETVQVAKSNVDLATTALDQTQERFRAGVSDNLPVSEAQSTLAAAQSQYVNSV
                     YALNQARLGLARNLGIVDTQYRTFLQGGNPPQVKSDQAAGMPKE"
     gene            9354..11135
                     /locus_tag="VHE33_00165"
     CDS             9354..11135
                     /locus_tag="VHE33_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95887.1"
                     /translation="MDAVYRRRLAEGQVRTERVRRWHIWLGYAKLADFLAAIIVAAFL
                     IRMHGPLLLLLIPLLIFVALIIAHDRVIRRLAKLTRITEFYSRGLARIQDQWAGSGET
                     GDRFFDPRHPYARDLDLFGKGSLFELLCTTRTRMGEETLARWLLKAADPEEVRARQQA
                     GAELRDRLGMREQLFLAGEDVRAGVHPEPLAAWGDGRPLLQSRALPWLLAVLAAAWVA
                     SIVIWILTRNWDVTLLMSLINYAVSYFLQRQVNESAGAVEKAAPDLKVLAEVLQIIER
                     EPFSAERLEGLRARLDTHHIAASQAVAKLERIYDWLEDRRNGFLVLFNAFVFYTAQLT
                     MAAERWRARFGGAIRGWLEVVGDFEALAALAGYTYEHPADVFPEMCSETPCFDAECLA
                     HPLLPAGRVVGNDLHLGASPQLIVISGPNMAGKSTFLRGVGLNAVLAQCGAPVRARRL
                     RMSPLTVGASICVLDSLQGGVSRFYAEISRLKVLSDLAGGPAPLLFLLDELLSGTNSH
                     DRLEGTKFVVQELVRRGAIGLVTTHDLALTAIPETMNGAATNCHFEDTIEEGQLKFDY
                     RLRPGIVQTSNALKLMEAVGLKVGSGQ"
     gene            complement(11159..11977)
                     /locus_tag="VHE33_00170"
     CDS             complement(11159..11977)
                     /locus_tag="VHE33_00170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HVT95888.1"
                     /translation="MKSQIANWDKYRVAPASKVRLRGLSTRDTSNCQDKPTARQLLKA
                     CRKEIDALLTTLAAEQARSLLVVLQGMDASGKDGTVRKVFTGVNPQYCSVVSFKQPGP
                     SEQMHDYLWRIYASLPEKGRAGVFNRSHYEDIVVPGARESHPLRLIHSRLDQVRDIER
                     IWCENGMTIVKFFLHISSAEQKRRFKARLNNPDKHWKVQESDFSDRRLWRRFEHIYQE
                     TLSRTSTKAAPWYVIPADHKWYRDLAIASIVRDTLDAMRPRLPHPKLNRGRFRL"
     gene            complement(12040..12396)
                     /locus_tag="VHE33_00175"
     CDS             complement(12040..12396)
                     /locus_tag="VHE33_00175"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT95889.1"
                     /translation="MAQATGIGGIFIRARDPKALYAWYEKHLGLPLQHGSFAIPAEHE
                     RAMIVFAFFGEKDAYFPVSQPAMVNLQVDDLDKLLEKLMAEGVQVDPKRETVDYGKFG
                     WITDPEGNRVELWQPL"
     gene            complement(12398..12631)
                     /locus_tag="VHE33_00180"
     CDS             complement(12398..12631)
                     /locus_tag="VHE33_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95890.1"
                     /translation="MDTIRAGFPAYLYLASTQMPKKRVKKRTFSAVKAVKANARERVG
                     QPKPERILPDAPREEKRGQKHPATLVKLLSEDE"
     gene            12722..>13001
                     /locus_tag="VHE33_00185"
     CDS             12722..>13001
                     /locus_tag="VHE33_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95891.1"
                     /translation="MEERDFFDEKSEQRKHTLTCPHCGQPGEYEVRWLVRTRKAQLPR
                     HADERDRARFAKAQSYMVRQDDLVGCKNIRCRKRFEVTSLQSVAFLQSA"
BASE COUNT         2633 a         3611 c         4340 g         2417 t
ORIGIN      
        1 cgttgcgccc ctggtgagcc acgcgctggg cgcaatgctg ctgagcggcg cgagagagtc
       61 gcacctggac acgtcactcg acgtgcgggt gtttgccttt gcagccctga tggcggtgac
      121 ggcgacgctg gtgatcggcc tggtgccggc gcttcgcgcg acctcccgcg atctgaacga
      181 gcagatcaag agcgggcagc acagtacgca gaagcaggag cggcaccggt ggttgccgcg
      241 ggcactgatg tcggtagaag tagcgctcgc gctggtgctg gtggtgggcg ccggactgct
      301 ggcatcgacg ctggtgcggc tgtatcggtc cggcgagggg ttcgatcccc acggcgtgca
      361 aaacgtcgcg ttcagcatgg aaaagagcgg gatgaaccat gcgtcgctga tggccttcta
      421 ccagcagatc ggcgaaggac tacggcgggt gccaggcgtg actgcggtga gttttgcacg
      481 catcgtgccg ttgaccggtt atgtctggga cgaagatatg tcggcgtccg ggggcaaacc
      541 aaccgataca tatttcaacg ccgtggggcc ggggtatttc tcggcgatgc gcattccact
      601 ccgcgaagga cgggatttcg ggtggaatga gacgacggcc gaaggcggga agatcgtgct
      661 gaatgcgcag gcggcgaagc tgctgttccc ccatggaggc gcgctgggac gcatcgtaaa
      721 tcggagccgt gggccgaagg aatcgccgct gccttacgag gtggtgggtg tggtggggga
      781 tgcgaagtac gaggatctcc gttcccaggc tccgccggcc gcatatacgg cgatgactca
      841 ggaggagggg aaggactcac catcgtcgta ctacgcggtg gtgcggacga gcggggatgc
      901 cggatcggtg gcgtctgccg cgcgaaaatt agcgcagcat ttggcccccg gaattccggc
      961 gccggtaacg acgtccatga tgacgacggt ggacgattcg ctgagcgccg aacggatgat
     1021 ggccctgctc tctgttttct ttgcggcgtg cgcactgctg gtgacagcga tcggccttta
     1081 tggaacgctg gcctacgcga cggcacggcg aaccagtgaa atcggcatcc ggatggcgct
     1141 gggcgcgcgc cgctcgcagg tggtcgggtt ggtctgcagt caaaatgcgc ttgtggctct
     1201 cgtcggaacc gctgtggggc tgatcgctgc gctgctggca tcgaaggctc tggccagctt
     1261 cctgtacggc accggcgcac aggacccgtg ggttctgatc ggctcggtcg cggccctggc
     1321 ggtcatcgcg agcgcggcgt cgctgttgcc ggctctgcga gcagcgcgga tcaatcccgt
     1381 ggttgcgatt cgatgtgaat aaacccacgg tttcagccgt agtactgcag gtgggcccag
     1441 aactcctgcc acggctctcg caggccgcgg cagacgtaaa ggtccggctt gccaatcgag
     1501 gaggtacgaa tgcccactga gttggtgact cgtcccgcaa cgaagcacga ttcgaaatgt
     1561 tggcgcagga agtccggatc cccgtcgatc acgatgaggg tttgcggcgg cggatcccca
     1621 tacccgcgag cccatgagga attcataccg ctgatcgcgg gcggcagatg gtacgccgga
     1681 ccatacaaat caatggcgcc ggtttcaccg tcgtccccgg tcgcaatcgc cagcctcgat
     1741 tgatcctccg ccggcaggcc atcgcggatg tgcgccacct gggcgaccat ctcgggccag
     1801 ccgatttgag aattgaagtt gccgccggtg caggcgtccg caaaatgcca ccacgcagtg
     1861 ccgacgggag cgacaggaac agcgagggca gtgatcgaga gcccgctgat cacgaaggat
     1921 tgccaggtcg tactccgaac ccagcccgcc gcacctggcg acaacgttgc aagccagtgc
     1981 tcagcctgga ccgctccggc agcgaagagc atcgggtacg ccggggaaag atagtagtcg
     2041 cgcccgtgga cgacaaacaa ggtgagggcc ggaatgacat acatccagcc cagcaggcgc
     2101 caacgtttgc ccccgggctt gcggaagacg taccagagcc caccgagcca gaaaggcacc
     2161 gtgaccgggt tggtggtctt ccagagctga ttcaggagga agaaatcggt cgaaccctga
     2221 tgaatgtcgc gggcatgaat gctgcggaac cactggagcc agatgaagtg gtggtgcatc
     2281 tgccagagga agttgggcag acacacaaag acggcaacag cagcgcccgc ccagaaccag
     2341 agtgacttgt agcagcggcg atacggcgtg agaagaaggc cgacgaggat tccgacaatg
     2401 agaacgccga tggtgtatcg cgtcatgagg ccaaggccaa tcgcggctcc cacggccaga
     2461 aaccaccgtg gatcatctga agccaggagt cgcgtgatgc agtagccggc caggacccac
     2521 cacaaatagt cgaacgtcga atacgacagg aagccgccgt tgaagagact gaagccgcta
     2581 attgccacgg cagcggcggc gacgagctgc gcccagcgca ggccacccat ggcgcgagcg
     2641 gaaagacccg tgaggatcat cgccaggccc tgagcaacgg cgggtaaaaa gcgaaatccc
     2701 ctcagcgagc tcccgaagag gagcagttcc aggcggccca gaaacggcgt gagaggtgga
     2761 tataccacat atccccaggc gaggtgccgg gcgttgctgt aggtgagcag ctcatcgcga
     2821 tggaagccgt aacggccatt gacggcgatg tgcagaagcg caatggaaag cccggagaag
     2881 agtagaagcg ttgtatcgct gaggcgggtt cgcggagcgt tcatggcatg gatcgaatcg
     2941 aggtgcaggg atggtttaca cccgaagcgg cgacagatca gtgatcgctg ccaccagtcg
     3001 cgaaagggac tgatatgctg gcagcaatct cacctctgcc ttcgctgaaa ctctcggagc
     3061 acaccaggcg gtatggaagc tttagtcgaa ctggatcacg tctcgaaatc ctacgaagaa
     3121 aaagccgcag tccacgatct gagcctgaag atcgaggccg gcaccatgtt cggcctgctg
     3181 ggacccaacg gcgccggcaa aacctcgacg atccgcatga tgatcgggat cacgatgccc
     3241 gatgcgggca aggtctcgct gttcggcaaa cccttcggca atcacgctct gaagcgggtg
     3301 ggctacctgc cggaagagcg cggtctgtac aagaaaatga aggtgatcga ccagctggta
     3361 ttcctggccg aattacgcgg tctgggagca ctggaggcga agcggcgtgc caaacagtgg
     3421 tgcgaaaagt tgcagatcgc cgactcgatc gaaaagagaa ctgaggagct gtcgaaaggc
     3481 atgcagcaga agatccagtt catctccaca ctgctgcacg atccggagct gatcatcatg
     3541 gacgagccgt tctcgggcct cgatccggtc aacgccgtcc tgctgcagga cacgctggtc
     3601 gagctgaaga agaacggcaa cgcggtgctt ttctccaccc accgcatgga ccaggtggag
     3661 aagctttgcg attcgatttg cctcatcaac cgcggcgagg cggtgctctc cggcggcatg
     3721 cgcgaggtga aatcgcgcta cgagcgcaac cgggtgatcg tgcagtttga ggggaacgac
     3781 gaatttttag ggcatcctgc aatcgccgac gcaaaaagct atgttggtca cgcagagatc
     3841 aagttgaagc cgcatgccga cgcccaggat ctgctgcgaa ccgccgcggc aatggcgact
     3901 atcacgaagt tcgagctggt ggaaccgtcg ctcgaggaga ttttcatcga aaccgtagga
     3961 gggcgcgccg atgcgtgatg gcttgctgcg gaacacgctg ctgattgcga agcgcgagta
     4021 cctggagcgg gtacggagcc gcgcttttcg catcatcacg ctgctggtgc ccattggcat
     4081 ggcggggatg atctttctgg tatcgatgag cgggaagaag ctggagggcc tgcagaatct
     4141 ggcgattctc gcggacgacc cgggcctggc acagcaggtg aaggcggcgc tggaggacgg
     4201 tgcgttcaag ccgaatcggg tggaagttta cgctccggcg accgatgccg acctgtcgcg
     4261 cgtgaacgga gagatagcga cgcatcagat tcagggatat ctgaaactga aaaccgtcga
     4321 gggagagaag gagcctgatg caacctggat ttcgggcagc tcgatcaacc tggtggggaa
     4381 ggagcagacc gagtcggcgg ttcgatcggc gctgatccgc agggagctcc tggcgcgcgg
     4441 catgagggaa gatcaggtcc acgacctgat gcaggaaacg aagctggaga cgaagcaact
     4501 gaagaacggc acggcggttt cctcggattc ggataagagc ttcgccggag ccgacgcgct
     4561 gatcatcctc ttgtacgcat cggtgctgat ttatgggatc aacatcgcgc gctcggtagt
     4621 cgaggaaaag acatcgcgca tcttcgaggt gctgctgtcc acggcttcgg cggactcgct
     4681 gatgctgggc aagctgttcg gagtcggagc agtgggtctg acacagatgg gaatctgggt
     4741 cggactggtg gtgctctatg ccggatcggc aatggcggcc agccagggga tccacgggct
     4801 caactcgctg gggcttaccc gcgtcgagct gatgttcttt ccatttttct tcctcggcgg
     4861 ctttttgctc tacagtgcac tggcggcggc gctgggatcg agcgtgagcg gggaccagga
     4921 aattcagcag ttcagcttca ttctgatttc tccgctggtg gccagcgtgg tgatgctgcc
     4981 ctacgtgctc ggcaatccca actcaacggc ctcgatcgtg ctgtcgctgc ttccgccgtt
     5041 cacgcccatc attatgtaca tgcgcatctg cgcacagatg ccccccgcat ggcaggtgtg
     5101 gctctctgtc gcgttgctcg cggcttcggt ttgggcaatg atttggctgg cagcgagaat
     5161 ctaccgggtc ggcgtgctca tgtacggaaa acgcgcaacg ctgccggaga tgatccggtg
     5221 gttgaagtac agctgatagg cagccggtag cccaactgtc cttcagggaa ccggatcgcg
     5281 cgtgcccatg ggcaccgact gccggcagga acgaatgcgg cggaaactga gcgcaaggcc
     5341 cttcgcaatc ccgtacttcc gaaccgcttc gaccgaatag cgggagcagg tgggctggta
     5401 gcggcagtgg atccagcggc cagtgatcgg atgcagatag tggtggtagc ctgcgacggc
     5461 cgcggtgaaa acccgcacgc tcacctgacg cgacggcgtg cggaaagaat cggcggccac
     5521 caacgcggcg aagaccagaa agaccgcgag ccaggtcgcc ggatgacgca gagctttgcc
     5581 tgggggcggc acggcgattc tctcaggctc cgcagttcgg gcacttcacc gctgcctgca
     5641 ttcgcttatt tccgcagttc ggacaaacaa caagattgcc ctgcgggcgc gccagcagat
     5701 agacgatcag gccgacgacg cccgtgaaca gaacgagcag catccacagc acgccattat
     5761 ccaggccgcg cgccttggca tcgcgagcca cccataccag gatggcaatg ttgagggcaa
     5821 tgatcgccag gaaaaacagg agaaagaagg agccgcaaat gccgcagccg gctgcgcctg
     5881 cagccgctga cgcatcgtcg ttttgagcaa gtgcagagcc ggtgaagagg gcaattccta
     5941 agcaaagcag agacaggccg ggcttcctca tgggcactct ttcggtgttc agttttgggg
     6001 agaaagctat tgtgcggcag tcggccagat gcaggcaata gaaaaccgcg aatcagcaag
     6061 cgccgattcg cggtttccat actggaatgc tgataagtca ggccccgcgc gaacggcggg
     6121 cgctttcgcc gccccgggag atgtctgcag ccgcaccgga cgagccgggc gcgggaaagg
     6181 gtgttaccga tccggagccg ccattctcgc cagcaccatg cggtgggcga tccaccgctc
     6241 gcggccggag gttcgtgggc aggaaaatgg tgaagacggt gccgtggcga tcagggtgaa
     6301 cagacgaacg caactgcatt tcaccgccat aacgctggat gatggagcgc gtcacccaaa
     6361 gtccaagacc ggttccacgc tggcctttgg tggtgaaaaa gggctcgccc agcctgcgct
     6421 ggacctcagg agggatgccg ctgccggtgt ccgcaacgga tacgcggata ccgcgtacct
     6481 gcggatcgct ccagctgcgc gtactgcgga cgcgcaggcg cagccgtccc cgcagggagg
     6541 atgcctcgat ggcgttcgtg accagattgg acaagacctg gcgcatctcg ccgggataga
     6601 caatcgcttc ttcgccgcaa tcgtactggc gttccacgcg aaggttcttc tccatcatgc
     6661 gccgcccata gagcgacagc acttcttcca gcaattcttc aatgtgaacc ggcaccgggc
     6721 cgctcgtctc gcgcgaaaac ttcagcgtct gccggctgat ctgggcgacg cggtccagtt
     6781 cccgctgggc gagagcaaga tagctcgtta cggtcgggga taaactcttc tcttcgccaa
     6841 gcaggaagag cagattcatc accgattcca gcggattgtt gatctcatgc gcgatagatg
     6901 cggcaagacg gccgatggtg atgagcttct ggttttcgcg cggcatgacc gccgagcggt
     6961 ccagttcgtg ctgggcatgg ctttgcacga ttgcggactg gcgcacttta agggcgcgag
     7021 cgatggcaga cggcagccgt tccaactctc ccgactgcac gatttcagtg gctccatcgc
     7081 ggagcatgcg aaccgccccc gcttcaccgg tgcgccctcc ggccagcagg acaggtatag
     7141 gtatgtctgc ggaccgggcg cgatcaatca cctcccggac gtccagatcg gttagaccct
     7201 cggttccggc aatgatgaga tcggtcgaac cgcggtgcaa ttcctccagg aattcgcggc
     7261 gattctcgac aaactccagc gttagccgga gccccgcgtg ccgtgccgct gcacgaacgg
     7321 agtgatgcga caccgccgca cagcgcagca tcaggactgc cgggatggaa gagcctgtca
     7381 tggtcacacc aggagtcggg caaatctcgg cgagcctctt cgccccgttc ggtatttctc
     7441 ttacggaagg cctctcggct tccaccagtt ccatacgagc ctccgggagt tcgcgctgtt
     7501 cccacatgca atgacccggc caatcgaaca cttggaaacc agccaggcgg tagttggcgt
     7561 ttcaggagcc cgcaacgtcg tacacaaaaa tttcgatgtg aggggcgcag ggagtgttgc
     7621 ttgactactt acggcctgac aggtggaaaa catggatttt gcggtacttg gcaggagctt
     7681 tcgttcgccc aggtgacggt tggcatgtca ggcgactttt ttggcaacgc agagcgggta
     7741 ttctgatcta aacagaagaa tctgcatgaa ttcagaggcg atgaagacac gtgtagcagg
     7801 cataatcgtg gggggtgccc ttttgctggg agcagcagcg ggggcgcaga gcaatccggg
     7861 taattcggcc gccaatcctt atatgggcag cgtgcaggcc gtggcgtttt cgccggagcc
     7921 gaaggccctt tccctggacg atgcgatcaa actcgggatt cagaacaatc ttgcgctgac
     7981 cctggcacgc gaacagcaga aggccgcgga cgcgacgaaa ctgcagctgg taaccgtgct
     8041 gctgccgaaa attaccttgc acggcgaaac gggcgtgcac cagtacaacc tggaggcgct
     8101 cggattccat ccggcatccc tgagcgattt cgcctcgcat ttgccaccgg gagcgagcgg
     8161 gtcgtttccc ctggtgacga aggtcgatac gaccatcgga gaggtgaatt tctcgcaggc
     8221 tttgttcaac tgggctgggt acgatgtgtg gcgcgcgtcc caggcctaca caaaggcggc
     8281 ctactataac gcgcagtcgt cccgcgggct ggtcgtcctg aatgtgggca atacgtatct
     8341 gcaggcgctc gcggctaaag cgcggctgga atatgcgcag gcactggtaa agaccgacga
     8401 gacggctttt aaccaggcgc acgaggaaca cctcgcgggg gtggtggcca atctggatga
     8461 actgcgggcg agggtccagc tccagagcca gcagcaggca gtgatcgcgg ctcagaataa
     8521 tttcgataag gcgaagattg ccctgaatcg cgagatcgga ctcaggccgg aacaggcgat
     8581 catactgacc gaatccagcc cgtatgccga gctgcacccc atggatctcg tggatgcgcg
     8641 catcgaggca tacgccaatc gacaggatta tctgggactg aaacaggaac tgatcgtggc
     8701 cgaccgggag cgggccgcga cgcggcacga gcgcttccct accctgacgt tcggcggcaa
     8761 ttggggagtg accggcgtta ccgggggggt ctatcacgag acgtttgccg ccgtgggcac
     8821 gctgagcatc cccctgttca aagaggcgga atttcgaggc gatcgcgacg tggcggaggc
     8881 tcaactggag gaaattcgct cgcggctgga agatctgaag gagaagatcg atcagcaact
     8941 gcgtgacagc ctcctggacc tgcagacggc gacggagacg gtgcaggtgg cgaagagcaa
     9001 tgtggatctg gcaaccaccg cactcgatca gacacaggag cggtttagag ccggcgtgag
     9061 cgacaacctg cccgtatccg aggcgcagtc gacgctggcc gcggctcagt cgcagtatgt
     9121 gaacagcgtc tatgcgctga atcaggcccg gctgggcctt gcccgcaatc tggggatcgt
     9181 ggacacgcag taccgaacgt tcctgcaggg cggtaatccg ccgcaggtaa agagcgacca
     9241 ggctgccgga atgccgaagg aatagacggt cgccagcgat cagcgagacc gttccctggt
     9301 cccctgatcc tgccgaatgc tcccgcccgt gcgattctta gatcgtggaa tcggtggacg
     9361 cggtttatcg ccgacggttg gcggagggcc aggttcgcac ggaacgggtg cgccgatggc
     9421 atatctggct gggctatgcc aagctggcgg atttccttgc cgcgatcatc gttgcggcat
     9481 ttctgatccg gatgcatggg ccactgcttc tgctcctgat tcctcttctc atctttgtgg
     9541 cgttgatcat cgcgcacgac cgggtgatcc ggcggctggc aaagctgact cgcattaccg
     9601 agttctattc acgcggactg gcacgaatcc aggaccagtg ggccggaagc ggcgagaccg
     9661 gcgaccggtt cttcgacccg agacatccct acgcgcgaga tctggacctg ttcggaaagg
     9721 gctcgctgtt cgaattgctg tgcaccacgc gcacgcgaat gggcgaggag acactggcgc
     9781 gatggctgct aaaggcggcc gatccggagg aagtccgagc gcggcagcaa gcgggagccg
     9841 aattgagaga tcggctggga atgcgggagc agttgttttt ggcgggagag gatgtgcggg
     9901 ccggcgtgca tccggaaccg ctggcggcat ggggcgatgg caggcctttg ctgcaatcgc
     9961 gcgctttacc gtggctgctc gcggtgctgg cggcggcctg ggtggcgtcg atcgtgatct
    10021 ggattctgac gcggaactgg gatgtgacgc tgctgatgtc gctgatcaac tacgcggtga
    10081 gttacttcct gcaacggcaa gtgaatgagt ctgcaggcgc agtggaaaag gcagcacctg
    10141 acctgaaagt tctggccgag gtactgcaga tcatcgagcg cgagccgttc tcggcggaac
    10201 gccttgaagg cttgcgggcg cggctggaca cgcaccacat tgcggcgtca caagccgttg
    10261 cgaagctgga gcggatctac gactggctcg aagatcggcg gaacgggttt ctggtcctct
    10321 tcaacgcttt tgtgttttac acagcgcagt tgaccatggc cgcagagcgg tggcgggccc
    10381 ggtttggagg cgcgattcgc gggtggctgg aggtggtggg cgacttcgaa gcgctggcgg
    10441 ctctggcggg gtatacgtat gagcatcccg cggacgtgtt tcctgaaatg tgctcggaaa
    10501 ctccatgctt tgacgcggag tgcctggcgc atccgctttt gccggcgggg cgagtggttg
    10561 gaaacgatct gcacctgggg gcgtcaccgc aactcatcgt gatcagcggg ccgaatatgg
    10621 cgggtaagag caccttcctt cgcggggtgg gtttgaatgc ggttctggca cagtgcggcg
    10681 cacccgtgcg ggccaggcgc ctgcgcatgt cgccattgac tgtgggcgcg tcgatttgtg
    10741 tgctggattc gctgcagggc ggggtgtcgc gcttctacgc ggagatttcc cgtctgaaag
    10801 tcctgtcgga cctggcgggt ggtccggcgc ctctgctgtt cctgctggat gaattgctgt
    10861 cgggaaccaa ttcgcacgac cggttggaag gaacgaagtt tgtggtgcag gaactggttc
    10921 ggcgcggcgc aatcggactg gtgacgacgc atgaccttgc gctgacggca attccggaga
    10981 cgatgaacgg agccgcaacg aattgccact tcgaggacac catcgaagag ggtcagttga
    11041 agttcgatta ccggctgcgg ccgggaatcg ttcagacgag caatgccctg aagctgatgg
    11101 aggcggttgg cctaaaagta gggagtggcc aatagccaac agggtcagat caaacagatc
    11161 agaggcggaa gcggccgcgg ttgagctttg ggtgcggtag acgcggccgc atggcgtcga
    11221 gggtatcgcg cacgatggat gcaatggcga gatcgcgata ccacttgtga tccgccggga
    11281 tgacgtacca gggcgccgcc tttgtggacg tacgactcag cgtttcctgg tagatatgtt
    11341 caaaacggcg ccaaaggcgg cggtcagaaa agtcggactc ctggaccttc cagtgcttgt
    11401 ccggattgtt gaggcgcgcc ttgaatcggc gcttttgctc cgcagaactg atgtggagga
    11461 agaacttcac gatcgtcatg ccgttctcgc accagatgcg ctcgatgtca cgaacctggt
    11521 ccagacggga gtggatcagt cggagtggat gcgactcgcg ggcgccggga acgacgatgt
    11581 cctcgtaatg cgagcgattg aagaccccgg cccggccttt ttcgggaagc gaagcataga
    11641 tgcgccagag ataatcgtgc atttgttccg agggaccggg ttgcttgaac gagacgacgc
    11701 tgcagtactg cgggttgacc ccggtgaaga ccttgcggac agtgccgtcc ttcccggatg
    11761 catccatgcc ctgaagaacg acgagaaggg agcgagcctg ctcggcagca agggtagtga
    11821 ggagcgcgtc gatttctttg cggcaggctt tcaggagctg gcgggcggtg ggtttgtcct
    11881 ggcaattcga cgtgtcacgg gttgaaagtc cgcgcaggcg gactttcgaa gcgggagcaa
    11941 cgcgatactt gtcccagttg gcgatttggg atttcaaaag ggccaaatcc tgaaccgagc
    12001 agcacgaggg gaccgtgctg ctcagactct acgccggcgc tacagtggct gccagagctc
    12061 gacgcgattg ccttcagggt cggtgatcca gccaaatttg ccgtaatcga cggtctcccg
    12121 tttggggtcg acctggacac cttcggccat cagcttttcg agcagcttgt ccaggtcgtc
    12181 gacctggagg ttcaccatcg caggctggga caccggaaag taggcgtcct tttcgccaaa
    12241 gaaggcaaaa acgatcatgg cccgctcatg ctctgcgggg atggcgaagg agccgtgctg
    12301 aagtggcagg ccaaggtgct tctcgtacca cgcgtagagc gctttgggat cgcgggcgcg
    12361 aatgaaaatg ccgccgatgc cggtggcctg agccatgtta ttcatcctcc gaaagaagtt
    12421 ttaccagggt tgcagggtgc ttctgaccgc gtttttcttc ccgcggggca tcgggcagga
    12481 tgcgctcggg tttgggctgc ccgacgcgtt cgcgcgcgtt ggctttgacg gccttgaccg
    12541 cagaaaaggt ccgctttttg acgcgtttct ttggcatttg tgttgaggcc agatacaaat
    12601 acgccggaaa cccagcacgg atggtgtcca cggcgtgttt ccgattgtcc tataaaaccg
    12661 tcttcgccgt gggttcaatg tgaaatactt cccatggaat cttttattgg agctgtaacc
    12721 gatggaagaa cgtgattttt ttgatgagaa gtccgagcag aggaagcaca cgctgacgtg
    12781 tccgcactgt gggcagccgg gcgagtacga ggtccggtgg ctggtgcgaa cgcggaaggc
    12841 tcaactgccc aggcatgcgg acgagcgaga ccgggcgcgg tttgccaagg cgcagtccta
    12901 catggtgcgc caggacgatc tggtgggctg caagaacatc cggtgccgga aacggtttga
    12961 ggtgacgagc cttcagtcgg ttgcgttcct gcagtcggcg t
//
LOCUS       DASZYN010000008         4012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_835621, whole genome shotgun sequence.
ACCESSION   DASZYN010000008 DASZYN010000000
VERSION     DASZYN010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835621"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VHE33_00190"
     CDS             <1..934
                     /locus_tag="VHE33_00190"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HVT95892.1"
                     /translation="ARGSELWNLYGPTETTIWSAAHRVRSGENPILIGRPIANTQMYI
                     LDENFQPVPIGVSGELFIAGDGVARGYRGRPELTASRFLADPFDSAGTRRMYRTGDLA
                     RYLPDGQIHLLGRTDHQIKLRGHRIEPGEIEAAIERNPYVRQAVVVLDGQGTSKQLIA
                     YVRYAEASGDVEQIRSWLRERLPEYMVPAILVPLDEFPMTPNGKIDRKRLPSPLTVAR
                     ETRADSIAARNQTEQRLTELWSEALHVDRPGIRDNFFDLGGHSLLLVQLHARLKREFS
                     SNLAVVDLFRYPTIEALASFLDRSAAATASPVLR"
     gene            998..>4012
                     /locus_tag="VHE33_00195"
     CDS             998..>4012
                     /locus_tag="VHE33_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HVT95893.1"
                     /translation="MRGPKPWPDGIAIIGMSARFPHSHSVQEFWQHLVAGDSLIDTFT
                     DEELRNSGVGTSMLADSSYVRRGNAIEDADRFDAEFFGLSRREAEILDPQQRVFLECA
                     WEALEDGGYTGEGERVGVFAGVGMNTYVMQLLKNPDVLASVGGYQMMLASDKDFLATR
                     AAYRLNLRGPAVTVQTACSTSLAAVHLACQSLLSRECCMALAGGVSITFPQTTGYAYI
                     PGMILSPDGYCRPFDERAKGTVPGRGAGIVLLKRLSEAIADGDSIVAVIRGSAWNNDG
                     AGKVGYTAPSVDGQAEVIRAALASAGVDSTRIGYIETHGTATELGDAIECAALAQVFE
                     KRQRPLILGAVKANMGHADAAAGVAGLIKAALAVESGIIPPTPNFTRPNPNLGLDTAS
                     FIVSSSSVGWTGENERWAGVSSFGIGGTNVHVVLSEAPASSPRNDEPAQSHVFPFSAR
                     TASALNTACEQMSQALTAEPSLSPADVASTLQRGRRPFAFRRAVVADNCADAATLLQK
                     PPKKFEPGIDLSRDVVFLFPGQGQQFPGMTAALYRQDASFRRIIDDGCALLKQHAGLD
                     LRGVLTGLGNTPETTTALRETHVAQPALFLVEYALAQRWRALGVEPACLLGHSLGELT
                     AACVAGVFSFEDGLRLAAERGRLMAQTTPGLMLAAMLPPEKLAPFLDKDLWLAAENGP
                     KMSVASGPVEAVESLERRLAAENVATVRLASKNAFHTPLMADAAKAFRAAVSATDRHA
                     PQIPWMSNVSGTWIGADEAQDPQYWANQIVSPVRFTRNLAALAQTRRLLLEVGPGEAL
                     IGIARQQLSTSVMVPSVGSENRRTSDAVIFLQAVARAWECGVSIQWERLEPATSGRRV
                     PLPTYPFERQRYWIEPARSTPDVATAPAPSALSSSISSGDSNAKRDDISTWFYAPSWR
                     STPPAAIAVPRREPPVASWLVLAGDDPLSNALTAALRSQGAGVVSVRSGERFAWQDAD
                     AIVNLSSAADLNLLWERIDAAGLHPEGLICLSG"
BASE COUNT          727 a         1385 c         1136 g          764 t
ORIGIN      
        1 ggcacgcggc agcgaactct ggaatctgta cggacctaca gagaccacca tctggtccgc
       61 tgcccatcgt gttcgcagtg gcgagaatcc catcctcatc ggacgtccca tcgccaatac
      121 gcagatgtac atcctggacg aaaatttcca gccagtgcca atcggcgtga gcggcgaact
      181 ctttattgcc ggcgacggag tcgcgcgcgg gtaccgcggc cggcccgagt taacagcgag
      241 ccgctttctg gccgatccat tcgattcggc cggaacccgc aggatgtatc gcaccggaga
      301 cctggcgcgg tacttgcccg acgggcaaat ccatctgctc ggccgcaccg atcaccagat
      361 taaattgcgc ggccaccgca tcgagccggg agaaattgag gcggccatcg agcgcaatcc
      421 ttacgttcgc caggctgtcg tcgttctcga cggccaggga accagcaagc aactcatcgc
      481 ctacgtccgc tatgcggaag catccggcga cgttgagcag attcgttcct ggctccgcga
      541 acgtctgccc gaatatatgg tgcccgccat tcttgtgcca ttggatgaat tcccgatgac
      601 ccccaacggc aaaatcgacc gcaagcggct gccttcaccg ctgacggtgg cgcgtgagac
      661 ccgtgccgat tccatcgcgg cgagaaacca aaccgaacaa cgcctcaccg agctttggtc
      721 cgaagcgctt cacgtcgatc ggcccggaat tcgcgacaac ttcttcgatc tcggcggaca
      781 ttcgctgctc ctggtccagc ttcacgcccg tctcaaacgc gaattcagca gcaacctcgc
      841 cgtggtggac ctgttccgct accccaccat tgaggcactc gcatcgtttc tcgatcgcag
      901 cgccgccgcc accgcctcac ccgttctacg ctgacatccc atgaactcat ctgacgccat
      961 ccccccagcg acatccgcga cagcccagga acccgcagtg cgcggcccca aaccctggcc
     1021 agacggcatt gccatcatcg gcatgagcgc gcgctttccg cattcgcaca gcgtgcagga
     1081 attctggcaa catctggtgg ccggtgactc tctcatcgac acgttcacgg atgaggaact
     1141 gcgcaacagc ggcgtgggca catccatgct cgccgattcc agttacgtcc gccgaggcaa
     1201 tgccattgaa gatgccgatc gcttcgacgc ggagttcttc ggcctgagcc ggcgcgaagc
     1261 cgaaatcctc gatcctcagc agcgcgtgtt cctcgaatgt gcctgggaag cgctggaaga
     1321 cggcgggtac acgggtgaag gagagcgtgt cggtgttttt gccggtgtcg gcatgaacac
     1381 ttacgtgatg cagctgttga agaaccccga cgtgctggcg agtgtcggcg gctatcagat
     1441 gatgctggcc agcgataagg atttccttgc cacccgcgcc gcttaccgtc tcaacctgcg
     1501 cggccccgca gttactgtcc aaacggcttg ttccacgtcg ctcgcagccg tccacctcgc
     1561 gtgccagagt cttctcagcc gcgagtgttg catggcgctg gccggaggcg tctccatcac
     1621 atttccccaa accaccggat acgcctacat ccctggcatg atcctctctc cagacgggta
     1681 ttgccgtccc ttcgacgagc gcgcgaaggg gaccgtccct ggccgcggcg ccggaattgt
     1741 tctcctcaag cgtctctccg aagccatcgc cgacggggat tccatcgtag cggtcatccg
     1801 aggctctgcc tggaacaacg atggcgcggg caaagtcggt tacacggcgc cgagcgtcga
     1861 tgggcaggcc gaggtcatcc gcgcggcact cgcctcggcc ggcgtcgact caacccgcat
     1921 cggttacatc gaaacgcacg gcaccgctac cgagctggga gacgcgatcg aatgtgccgc
     1981 tctcgcccag gtattcgaaa aacgtcagcg gccgctgatc ctcggcgcgg tgaaagcgaa
     2041 catgggccac gccgacgcgg cggccggcgt cgccggcctc atcaaagccg cgctcgcagt
     2101 ggaatccggc attattcctc ccacgcccaa cttcacccgg cccaatccca atctcggcct
     2161 cgatacagcc tccttcatcg tctcctcctc ctcagtgggc tggacaggcg aaaatgaacg
     2221 ctgggctggc gttagttcct tcggaattgg cggcaccaat gtgcacgtcg ttctgagcga
     2281 agcgcctgcc tccagcccgc gcaacgatga gccagcgcaa tcgcacgtct tcccgttctc
     2341 tgcgcgaacc gcctccgcgc tgaacaccgc ctgcgagcag atgtcgcaag cacttacagc
     2401 ggagccgtcc ttgtctcccg ccgatgttgc ctcgaccctc cagcgtgggc gccgtccctt
     2461 tgcattccgg cgagctgtcg tcgccgacaa ctgcgccgat gcagcaacgc tccttcagaa
     2521 accgcccaaa aagtttgagc ccggaatcga tctcagtcgc gacgttgttt ttctcttccc
     2581 cggacaaggt cagcaattcc caggcatgac tgccgctctc tatcgccagg atgcctcctt
     2641 ccgccgcatc atcgacgacg gatgcgcgct cctgaagcag cacgccgggc tcgatcttcg
     2701 cggcgttctc accggcctcg gcaacacgcc cgaaaccact accgcgctcc gcgaaactca
     2761 tgtcgcgcaa ccagcactgt ttctcgtcga atacgctttg gctcaacgct ggcgcgccct
     2821 tggcgtcgag ccggcctgtc tgctcggaca cagcctcggt gaactaaccg ctgcctgcgt
     2881 cgccggcgtc ttttccttcg aagacggtct tcgtcttgct gctgaacgcg gacgtctcat
     2941 ggcgcaaacc actccgggcc tgatgctggc cgcgatgctt ccccccgaaa aactggcgcc
     3001 ctttctcgac aaggacctgt ggctggccgc cgagaatggc ccgaagatgt cggtagcgtc
     3061 cggccccgtt gaggctgtcg agagcctcga gcgcaggctt gcggccgaaa acgtggcgac
     3121 ggttcgtctt gcctcgaaaa acgcatttca tacgccattg atggccgacg ccgcaaaggc
     3181 ctttcgcgca gcggtgtctg caacggatcg gcatgcgccc caaattccct ggatgtccaa
     3241 cgtcagcggg acatggatcg gcgcagacga ggcacaggat ccgcaatact gggccaatca
     3301 gatcgtctca ccggtgcgat tcacacggaa tcttgccgcc ctcgcgcaga cccgccgctt
     3361 gctgctcgaa gtcggaccgg gcgaggcact gatcggcatt gcgcggcagc aactatccac
     3421 cagcgtcatg gtcccttcgg tgggttcaga gaatcgccgc acttccgatg cggtcatctt
     3481 cctccaggct gttgcccgcg cctgggagtg cggtgtctcc atccaatggg aacggctcga
     3541 gcccgcgacc tccggtcgcc gcgtgccgct gccgacctat cctttcgagc gccagcgcta
     3601 ctggattgag ccggctcgat ccactcccga cgtcgcgacc gctccagcgc cctcggcctt
     3661 gtccagctct atttcttctg gcgattcgaa cgccaaacga gacgacattt cgacctggtt
     3721 ctatgctcca tcctggagaa gcacgccgcc agccgccatc gctgtgccca ggcgagaacc
     3781 acccgtggct tcctggctcg tgctggctgg agatgatcct ctcagtaacg ccctgaccgc
     3841 cgctctccgt tcccagggag ccggcgtggt gtcggtgcgc tccggcgagc gctttgcctg
     3901 gcaggacgca gacgccattg tgaacttgtc cagtgcggcc gatctgaacc tgctttggga
     3961 acggatcgac gctgccgggc tgcaccctga aggtctgata tgcctgagcg ga
//
LOCUS       DASZYN010000009         1849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_578432, whole genome shotgun sequence.
ACCESSION   DASZYN010000009 DASZYN010000000
VERSION     DASZYN010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578432"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..348)
                     /locus_tag="VHE33_00200"
     CDS             complement(10..348)
                     /locus_tag="VHE33_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95894.1"
                     /translation="MSSARLAALEKQYATLRRQLAGIGYVSHGSVYRRPAGQSGSRFI
                     WSSKVNNKTVSLALSEPQADWLETAIEEHRKLKKLIADMHRISRQIMRLRFEDTERRK
                     PLNKRVLRLI"
     gene            455..1792
                     /locus_tag="VHE33_00205"
     CDS             455..1792
                     /locus_tag="VHE33_00205"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HVT95895.1"
                     /translation="MGVMFRVRAAADSINGLAVLFARFFGSDLCPARTGRGSRERNFG
                     RVAVFWAFLGQVLTRGSSCRWAVERLQAEALARGRRRPDDSTSGYCQARAALSLAWLQ
                     VLFARLGQWFAPRVHNAWLGRTVRVIDGTGFSMPDTPANRQQWDYPAGTKPGCSFPAG
                     KLVGLFCLHSGRLIAFVHDTWRTHDVKLARQLVCWLHRADVLIGDRAYCGWIFLALLR
                     RRQADFVIRLHQARRIRSRRSGSTFEAWGRPQRPAEHSRRFWRALPKELPVRLVRFIV
                     HPRGFRAHPVIVATSLLDQKAYPDAAIAKLYAQRWQVELHYRQIKTNLSLDILRGLSP
                     CMIERELWMSAIAYNLIRALLLEAAMTHAVPIERLSFKGALDAVHAWTSSPLLRRARR
                     RARTELLARIAADRVPIRPGRQEPRAIKRRHKNYQFLTRPRRRFRVSPSRSLK"
BASE COUNT          285 a          640 c          543 g          381 t
ORIGIN      
        1 aaatggcgct tagataaggc gcaaaactcg tttattgagg ggctttcgtc gctcggtgtc
       61 ttcgaagcgc aggcgcatga tttgccgtga gattcggtgc atatcggcga tgagtttctt
      121 gagcttacgg tgctcttcga tcgccgtttc gagccagtcg gcctggggct ccgagagggc
      181 caggctcacc gtcttgttgt tcaccttact tgaccagatg aagcgggagc cggattggcc
      241 tgccggccgg cggtacaccg agccgtggct aacatagccg atgccggcga gctgtcggcg
      301 cagggtggcg tactgcttct cgagcgcagc aagacgggcg gaggacatgc ttctcaggat
      361 cggtaaatgc gcgtgactcg tcaactaggc ggtagtacta cggcctatgc tttccacccc
      421 gtacctgccc gctcttcggc agtgctgcgc gcctatgggc gtgatgtttc gcgtccgggc
      481 cgcggccgat tcgatcaacg gcctcgctgt cttgtttgcc cgattctttg ggtccgatct
      541 gtgtcccgca cgcaccggcc gcggcagccg tgagcgcaac tttggccgcg tggctgtttt
      601 ctgggccttc cttggccagg tgctcacgcg cggcagttcc tgccgctggg cggtggagcg
      661 actccaagcc gaagcactgg cgcgtggtcg acggcggccg gacgattcca ccagcgggta
      721 ctgccaagct cgggcggcct tgtcactggc atggctccag gtgttgttcg ctcgcctggg
      781 gcagtggttc gctccgcgag tgcacaacgc ctggctcgga cggacggtcc gcgtcatcga
      841 tgggaccggc ttctcgatgc ccgatacacc cgcgaaccgg cagcagtggg attatcctgc
      901 tggaacaaag cccggctgca gctttccggc cggcaagctg gtcgggctgt tctgtctgca
      961 ttccggtcgc ctgatcgcgt ttgtccacga cacctggcgc acgcatgacg tgaaactcgc
     1021 ccgccagctc gtctgctggc tgcaccgcgc cgacgtcctc atcggcgacc gcgcctactg
     1081 cggctggatc ttcctggcgc tgctgcgccg ccgtcaggcc gattttgtca ttcgcctgca
     1141 ccaagcgcgc cgtatccgct cgcgccgcag cggttccacc ttcgaggctt ggggacggcc
     1201 gcagcgtccc gccgagcact cgcgacgttt ctggcgcgca ttgcccaagg agctgcccgt
     1261 gcggctggtg cgcttcatcg tccacccacg cggcttccgc gctcaccccg ttatcgtcgc
     1321 cacctctctg ctcgatcaaa aggcctatcc cgacgcggct atagctaaac tgtacgccca
     1381 gcgctggcag gtcgaactgc actatcgcca gatcaaaacc aatctctcgc tcgacatctt
     1441 acgcggcctc tccccatgca tgatcgagcg ggaactgtgg atgtccgcta tcgcttataa
     1501 cctgattcgc gccctcttgc tcgaagctgc gatgacccac gccgtcccca tcgagcggtt
     1561 aagcttcaag ggtgccctcg acgccgttca cgcctggaca tcaagcccgc ttctgcgtcg
     1621 cgctcgccgt cgcgcccgca ccgaactgct cgcccgcatc gccgccgatc gcgtcccaat
     1681 ccgtcccggc cgccaagaac ccagagcaat caagcgccgg cacaaaaact accagttcct
     1741 cacccgaccc cgacgccgct tccgcgtctc accctcccgc agcctcaagt gaccgatgat
     1801 attccgccac tcgaagctta tcttagcgcc atttcggcct gaccccgtc
//
LOCUS       DASZYN010000010        12149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_963816, whole genome shotgun sequence.
ACCESSION   DASZYN010000010 DASZYN010000000
VERSION     DASZYN010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 12149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12149
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963816"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VHE33_00210"
     CDS             complement(<1..681)
                     /locus_tag="VHE33_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95896.1"
                     /translation="MNFYTIRIESLHIDNTRSRHNDTDTVFLALQAGNTQFAVRSKAL
                     GDVNNGDHAVSLGFSSAMLSDPDRVLGFSFSIYNGDTAGLSDMLNHALTSLLTQGVHL
                     TVPGPEITSINISGAAAGAGVTANTVGITSLLANTSAWYAAAAKAAAEAAINFVFPNC
                     DGYVAADAFCLTHQQWNSVIDWGGNDVCRITMRYAGTNSPDGCGSNSNYTVTWSITRD
                     RVRGSMRSF"
     gene            complement(696..1448)
                     /locus_tag="VHE33_00215"
     CDS             complement(696..1448)
                     /locus_tag="VHE33_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95897.1"
                     /translation="MPDQKPFISPVAGLPGAVPVTEQAAHPPLVAYSTNNAAAVHGIG
                     QANGHAVLGVSAKGDGVHGQSQVANMSGVAGINTAGGNGVYGSSSGNAGCFDGNVQVN
                     GNVTISGDLFLPGADCAEHFDVAPDQTLMPGTLVALDESGALRRCDRPYDRTVAGVIA
                     GAGACRPGIILDQQRSASPRAALSLIGKAYCYADATHAPIVVGDLLTSSATPGHVMKA
                     EDPARSFGAVVGKALRPLGAGTGLIPILIALQ"
     gene            complement(1728..1804)
                     /locus_tag="VHE33_00220"
     tRNA            complement(1728..1804)
                     /locus_tag="VHE33_00220"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1768..1770),aa:Glu,seq:ctc)
     gene            1926..2396
                     /locus_tag="VHE33_00225"
     CDS             1926..2396
                     /locus_tag="VHE33_00225"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HVT95898.1"
                     /translation="MRLWIVAVGARPRDAFDELGRMYVDRIAPLLPGSGKSPVETPVY
                     RTEEAMWDAVERERSRAAPQVVLLDERGRSMSSEAFAKWMGHERDEGRQLLVFAVGPA
                     DGWSRDSLARAGERLSLGPMTLAHELARVVLCEQVYRALTILAGHPYHRGASGR"
     gene            2424..2969
                     /gene="cobO"
                     /locus_tag="VHE33_00230"
     CDS             2424..2969
                     /gene="cobO"
                     /locus_tag="VHE33_00230"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004637.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HVT95899.1"
                     /translation="MSETAETRKGLILINTGPGKGKTTAAMGTALRAVGNGMRVLMLQ
                     FLKGSWHYGELDSTKAFGDNFVLRQMGRGFVKIGGAETDPEDIRLVEEAWEEARRAIL
                     SGEWDMVILDEINYAISYGMLDPAKVVETLEARPEMVHVILTGRNAHASLVAIADTVT
                     EMREVKHAYQKGILAQRGIEY"
     gene            complement(3045..4559)
                     /gene="hutH"
                     /locus_tag="VHE33_00235"
     CDS             complement(3045..4559)
                     /gene="hutH"
                     /locus_tag="VHE33_00235"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HVT95900.1"
                     /translation="MSSPLLLSGQPLSLDEIGQVALHNRAVALDPAALPRIRESRAVV
                     DGLLARGATVYGVNTGFGKLSDVRIEPGEVQALQRNLVRSHACGVGDPLPESEVRAML
                     LLRANVLAKGLSGVRPAIVEMLLALLSHGIHPVVPARGSVGASGDLAPLAHLALAMIG
                     EGMALLEGRPMPAAMAMLKTGLRPLVLEAKEGLALLNGTQAITAVGSLALARALRVAE
                     LADLAGAMTLEALKGTPTPFDPRIHAARPHLGQVAAAAHLRDLLADSEIRESHRDNDP
                     RVQDAYCLRCMPQVHGAVRDALASARTTLEIETGAATDNPLVFPEGEILSGGNFHGAP
                     LALQLDAAAIAMTTLMGIAERRIDRLVNPDINEGLPPFLSKTPGVSSGFMIAHIVAAA
                     LLNEAKVLAHPASADSVPTSGGKEDHVSMGMTAALKFRQIVENSERLLAVELLCAAQA
                     LEYRAPLKPARRVGEAFARARQIVGPLAEDRVLSADIEAMAAALRNATFDDWRA"
     gene            complement(4679..5086)
                     /locus_tag="VHE33_00240"
     CDS             complement(4679..5086)
                     /locus_tag="VHE33_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95901.1"
                     /translation="MPRRRAAWIIVAAVLTLCAAMGWYCLAADYDYPSLAGTYVFQDD
                     AGSCTLRLYANRNFEEELKILGNVRIARGSWRRFGESGMALSDSFLKVPGQKPGPSGE
                     NYATFYKRLGIFPVLQIDSTSGRLQFHRKLINW"
     gene            complement(5101..6465)
                     /locus_tag="VHE33_00245"
     CDS             complement(5101..6465)
                     /locus_tag="VHE33_00245"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HVT95902.1"
                     /translation="MAISGTSSSQTIQVAPGGEGTSYRSALAMVTTLFFMWGFLTSLN
                     DILIPHLKSIFELNYAQVMLVQFAFFTTYALLGIPSGKVVEWIGYKKTMVAGLLTMGV
                     GSLLFVPAAKVPSYPLFLGALIVLAAGITALQVAANPYVTVLGPPRTASSRLNLTQAF
                     NSLGTVLGPIFGAFVILRTAGSVQDVTKMSPEVHRAYQIHEASTVNVPYVGIALALVL
                     LGLAIAMYKFPRIETTKDYRPSELHKEKGSIWSHSHVVFGALAIFAYVGAEVSIGSFL
                     INYFAQPEIGGLATTPGGGPFHAGSAAILQLATKIIGTWVPLYWGGLMVGRFIGSGLQ
                     QKVSPGRMLSVFALCAMTLTVVSMLTSGGLAVWSILLVGLFDSIQFPTIFTLGVAEMG
                     PLTGEASGLLITAIVGGAIIPELQGLLADRIGIHHAFVLPILCFLYIAWYGVRGSKVK
                     QPVA"
     gene            6705..7874
                     /locus_tag="VHE33_00250"
     CDS             6705..7874
                     /locus_tag="VHE33_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95903.1"
                     /translation="MSSPRHLRLAVAALCCIFAMAAASSAAAQAPASNKVIGTVTAVS
                     GTSVSVKQDSGSDATVNVPETANVVRTEPGSKTLAGATKIALADIEAGDRVLILVSGD
                     PPVAKVVVVNKKSDIAAKQQQEQADWARRGVGGLVKAVDGGAGTVTIAATNRTVTIHT
                     TASTVIRRYAPNSVKFTDAQPSTLAAIQPGDQVEARGDRSGDDVKADEIVSGSFRNIA
                     GTVSSIDSAANSFTVKDLVTKKSVTIKTTPDSDLRKLDPQMAQMVAMRLRGGGNGGGQ
                     TQQSSGEHAAGPGGSQVRQGGGGPANTGGQGVGFSRVLQHSPEIHVSDLHKGDAVMIV
                     ATSGDPDSATAIRLLAGVEPMLQASASGSQSMFSSAWSGLGGGGGDQGGGEGTSQ"
     gene            8093..11152
                     /locus_tag="VHE33_00255"
     CDS             8093..11152
                     /locus_tag="VHE33_00255"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HVT95904.1"
                     /translation="MGRWLLIGTFAGLLACVPSSPAQEAVAPTTPSVPQVTSGAPTQN
                     AGGIRGQVADPSGAVIPNATIVVLDTAGKTVGKTTSDAGGVYAVHGIPPGTYSVWVTA
                     EGFAAWRVPNVAVAAGQMKNVNPALQIQVQQQQVEVQAENTTIGTAADANASALVIKG
                     SDLNSLSDDPDELQNELQALAGPSAGPNGGQIYIDGFTGGQLPPKSSIREIRVNQNPF
                     SAQYDRLGYGRIEIFTKPGTDKLHGQAEIVGNTSAFNSQNPILQNRTEPSYYSWMLHG
                     SVGGPISKSASYFVSMFSRKQQDEDILQAIDPATVHFDSNGLATGSSINDAIDNPGSR
                     LNVSPRLDLQLGKANTLTVRYEFNRGVSTNGGLNALSLPSTAINTHQEDNTLQLSDSL
                     ILSPKLVDDIRFQYRRIRNSQTAVSSLPSYTVQQSFTAGGSAEQSVQDHENVFELQNY
                     FSGAFGAHSLNFGARVRAYDDVNASTSGSNGSYSFTDLASFVGCLDNPPAASCHPRQY
                     SYNYIQNPVASAILFDSGLFYQDDWKLNPRVTFSYGLRWETQNRIHDKNDWAPRVTVA
                     YALGRSSGKQQPKTVIRAGYGWFYQRFTVANGFGAQVPYIVNAIHQNGINQQRFIQNA
                     SNSTIAFNPNTPTVITSGSTGGGPAAPTKYTIDPNMKTANEMEAAAGVDRQLSKFMTG
                     NVTYVYSQGIHQYFTDNLSAAASEVAAGGFQDGEYPETQPDEPLENNLQYQSGGFYRE
                     HQLMATIRANYRAFSLMTNYTWSDAKGDTSGVGTVPSVSSNPGLDYGRTSFAVEHRFM
                     VFGNVTLPWKVSVSPMLVANSGNPFNVTTGSDLTGNNQFNARPTYADPSKCDGVNENY
                     ISAGHFGCLDINPYNTGNAGEKIIPYGVGTGPSSVSLNMRLNKSIGIGPRLGEGEHAA
                     GGDGGGFHGGPRGLGGGGFSGSRGGPGRMEAAVNRRYTLNLGVWATNVLNHENLGTPN
                     GVLTAGQSQGTGRPGSYFDQSQSLAGGFFGPSTSGNRSVFLQASFSF"
     gene            11230..>12149
                     /locus_tag="VHE33_00260"
     CDS             11230..>12149
                     /locus_tag="VHE33_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HVT95905.1"
                     /translation="MPNPSLGFATRAIHDGQAPDPRTGAVTVPLYLTSTYELQGIEQH
                     RGWEYSRVSNPTRDAVEASLASLEGGTSGHAFGSGMAAIAALVTMLKVGDHVICGHNV
                     YGGTTRLFTQVIPHHGIEFSFVDTSNAENVRQAMRPTTKLVHIETPTNPLMMLTDIRA
                     VAAICHEQGIPLSVDNTFMSPYFQRPLELGADVVMHSTTKFLNGHSDGLGGILVTTRS
                     DLAEQFRFVQKCTGGILSPFEAWLILRGVKTLAVRMRQHDANGRAVAEYLSKHPKVSN
                     VLYPGLTSHPQHELAKRQMSGFGSMIAFEVG"
BASE COUNT         2490 a         3697 c         3712 g         2250 t
ORIGIN      
        1 aaagctgcgc atacttcctc gtacgcgatc ccgagtgatt gaccaggtga ccgtgtagtt
       61 ggaattgctg ccgcagccgt cgggcgaatt cgttcctgca tagcgcatcg tgatgcggca
      121 gacatcgttg ccgccccaat cgatgaccga attccattgc tggtgggtca ggcagaaagc
      181 atcggcagcg acatatccat cgcagtttgg aaagacaaag ttaatcgccg cttcagcagc
      241 tgcttttgcg gcagcggcat accacgccga agtgttcgcc aataagcttg taataccgac
      301 ggtattggcc gtaacgccag ctccggcggc tgcgccggaa atgttaatcg aggtgatttc
      361 aggccccggg actgtcaaat gaacgccctg ggtcagcagc gatgtgaggg cgtgattgag
      421 catgtcagag agaccggcgg tatctccgtt gtagatgctg aagctgaagc caagaacccg
      481 gtccggatca gaaagcatgg cgctggagaa acccaacgac accgcgtgat cgccattatt
      541 gacatcgcca agtgccttgc ttcggacggc aaattgcgta ttgccggcct gcagcgccag
      601 gaagaccgtg tcggtgtcgt tgtggcgaga acgcgtgtta tcgatgtgaa ggctttcgat
      661 tcgaatggtg taaaagttca cgtcgacctc gtgcgttact gcagtgcgat cagaatcggg
      721 atgaggcctg ttccggcacc aagagggcgc agcgctttgc cgacgaccgc gccaaaggaa
      781 cgggctggat cctctgcctt catcacgtgg ccgggtgtgg cggatgacgt cagcagatcc
      841 ccaaccacaa tcggggcgtg cgtcgcgtct gcataacagt atgctttacc gatcaacgag
      901 agggccgcgc ggggagaagc ggatcgttgc tgatccagaa tgattccggg cctgcaggca
      961 cccgctccag cgattacgcc ggcgacggtg cgatcgtagg gacgatcgca tcggcgcaga
     1021 gcgccacttt catcaagcgc gaccagcgtg ccgggcatca gcgtctggtc gggcgctacg
     1081 tcgaagtgct cagcgcagtc ggcgccgggc aaaaagagat cgccgctgat cgtgacgttt
     1141 ccattcacct gaacattgcc gtcgaagcag ccggcgttcc cgctgctcga accgtagacg
     1201 ccattgccgc ccgccgtgtt gatacctgca acgcccgaca tgttggccac ctggctttgg
     1261 ccgtgcacgc cgtcgccctt tgcactgacg ccgagaacag cgtgtccgtt tgcctggccg
     1321 atgccgtgga ccgcggccgc gttatttgtg ctgtaagcga cgaggggcgg atgcgccgcc
     1381 tgttccgtga ccggaaccgc tccgggcaat ccggcgaccg gagagatgaa tggcttttga
     1441 tcgggcattt ggttgtgacc ttcgcagggg attcgaacgt atcgaagaga tacgcaccac
     1501 agatattcca ttgctacggc ttttgcaatg atttttccga tcaaccatca ggcgaaaaat
     1561 gtgtgtgcgc cgggccagag gaactcaacg catccgtcgg ccttaaccga gcgctgggcg
     1621 gggggcaaaa ctgggtcaag caggcaacag gcaggtgcga ccccgagtct tgacggagcg
     1681 atttcaatcc attcaggaag attggccgga cagccttgaa gggggcttgg tagcgccatc
     1741 ggggatcgaa cccgaactct tcgccttgag agggcgacgt gttaaccaat tacaccatgg
     1801 cgccgtaagg gttgggggcc ggctccgcaa acggatcggc ctctcccaat ataacaggct
     1861 tacacgggtc gatggcggag tgagagcgcg cgttttcgcg gcccatccct cgtagactgt
     1921 tgtccatgag gctctggatc gtcgccgttg gcgctcgtcc gcgtgacgca ttcgatgaac
     1981 tagggcgtat gtatgttgat cggatcgcac ccttgctgcc tggcagcgga aagtctccag
     2041 tcgagacccc ggtctatcga accgaagagg cgatgtggga cgcggtggaa cgcgagcgct
     2101 cacgcgcagc gccccaggtc gtgctgctcg acgaacgcgg ccggtcgatg tcgtcggaag
     2161 cctttgcgaa atggatggga cacgaacgcg acgagggccg acaactgctg gtgttcgcag
     2221 ttgggccggc ggacggatgg tcgcgggact cgctcgcgcg cgcgggtgaa cggctttcct
     2281 tggggccgat gacgctggcg cacgaactgg cgcgggtcgt gctgtgcgag caggtttatc
     2341 gcgcgctgac gattcttgcc ggtcacccgt atcatcgggg agccagcggt cgttaatggt
     2401 ggcggcgggt ttgtggagac aacgtgagcg agacagcaga gacgcgaaaa ggactgatcc
     2461 tgatcaacac gggaccgggc aagggcaaaa cgactgcggc gatggggaca gcgctgcgag
     2521 cggtcggaaa cggtatgcgt gtgctcatgc tgcagtttct gaaggggtcg tggcactacg
     2581 gggagcttga ttcaacgaag gcatttgggg ataacttcgt gcttcgccag atgggccgcg
     2641 gttttgtgaa gatcggcggg gccgagacag acccggaaga catccgcctt gtcgaggagg
     2701 cgtgggaaga ggcacgccgc gcgatcctgt cgggcgaatg ggacatggtt attctcgacg
     2761 agatcaacta tgcgatcagc tacggcatgc tcgatccggc gaaagtagtt gagacgctgg
     2821 aggctcgacc cgaaatggtg catgtgattc tcacgggccg caacgcacac gcgtctctgg
     2881 tcgcgattgc ggacacggtg acggagatgc gtgaggtgaa gcatgcctac cagaagggaa
     2941 ttctggcaca gcggggaatt gagtattaag cccttctcct gttcggcgta ccgatagcgc
     3001 tgacgcgtgg ccacagccat cagctcgccg ccagccaggg gggatcaggc tcgccagtcg
     3061 tcgaacgtgg cattgcggag agccgccgcc atggcttcga tgtctgcgct gagaacccga
     3121 tcttcggcca gcggcccgac gatctgacgc gcgcgtgcga aggcttcgcc gacccgccgc
     3181 gcaggtttca gcggcgcgcg atactccaac gcctgggctg cgcagagcaa ctccacagcg
     3241 agaaggcgct cggagttctc aacgatttgg cggaacttca gagcggccgt catgcccatc
     3301 gagacgtgat cttctttacc gcctgacgtc ggcacgctgt cagcgctggc tggatgcgcg
     3361 agcaccttgg cttcattgag cagggcggcg gccacgatat gagcaatcat gaaccctgaa
     3421 gacacgcccg gagtcttcga caggaacggc ggaagtcctt cgttgatgtc cggattcacg
     3481 agcctgtcga tgcgacgctc cgcgatgccc atcaacgttg tcatcgcaat cgcagccgca
     3541 tccagctgca gagcgagagg cgctccgtga aaattgccgc ctgaaaggat ctccccttcg
     3601 ggaaagacca acgggttatc cgtcgcggcg ccggtttcaa tttcgagagt ggttcgcgcg
     3661 gacgcgagcg cgtcccgcac cgctccgtgg acctggggca tacagcgcag gcagtacgcg
     3721 tcctggacgc gaggatcgtt gtcgcggtgc gactcgcgaa tctcgctgtc ggcaagcaga
     3781 tcgcgcagat gcgcggcggc tgcaacctgc ccgagatgcg gacgagcggc atgaatccgc
     3841 ggatcaaacg gtgtgggcgt tcccttgagg gcttcgagcg tcatcgcacc tgcgaggtca
     3901 gccagctcgg ccacgcgcag ggcacgtgcg agcgcgaggc tgcccacggc tgtgatggcc
     3961 tgggtgccgt tcagcagtgc caggccctcc ttcgcttcga ggaccagcgg ccgaaggcca
     4021 gtcttgagca tggccatggc cgcgggcatc ggccgccctt ccagcagcgc catgccctcg
     4081 ccgatcatgg ccagagccag gtgcgccagg ggcgcgaggt cgccgctcgc cccgaccgat
     4141 cctcgcgcgg gcacaacagg atggatgccg tggctcagca gagcgagcag catctcgacg
     4201 atggcgggac gtacgccgct gaggcctttg gcgagaacat tcgcgcgcag gagcaacatc
     4261 gcgcggacct cggactcggg cagggggtcg ccgacgccgc acgcatggct ccgcaccagg
     4321 ttgcgctgca gcgcctgcac ttcgccgggt tcgatacgaa catcggagag cttgccgaag
     4381 cctgtattca cgccatagac agtggcgccg cgggccagca gaccatcgac aaccgctcgg
     4441 gactcgcgga tgcgcggtaa ggcagcggga tcgagcgcga cggcgcgatt gtggagcgcg
     4501 acctgtccga tttcatccag agaaagaggc tggccggaaa gcaaaagtgg ggatgacatc
     4561 gggaaccagc gtacagcgtt cagggttcag cgtacaggtg cggcatacag ggctcggcct
     4621 atccgcctgc ttattggggg ttcgacgtgg ctcaacagca aatgactcca gagcgcaatc
     4681 accaattgat gagcttgcgg tggaattgaa ggcgaccaga agtggaatcg atctgcagca
     4741 cggggaaaat tcctaatcgc ttgtagaatg tcgcgtaatt ctcaccagag ggcccgggtt
     4801 tctgtcccgg cactttcagg aaggagtcag aaagagccat accgctctct ccgaagcgac
     4861 gccaactacc tcgagcgatc cgaacgttgc ccaggatctt gagttcttcc tcgaagttcc
     4921 gatttgcgta aagcctcagc gtgcaactgc cggcgtcatc ttgaaagaca taggttcccg
     4981 ccaggcttgg atagtcgtaa tcggctgcaa ggcagtacca gcccatggcg gcgcacaaag
     5041 ttaataccgc cgcaacgatg atccacgccg ctcgtctcct tggcatccga cggagcttac
     5101 ctacgctacc ggctgcttca ccttcgaacc cctcactcca taccaggcga tatacaggaa
     5161 gcagagaatc ggcaggacga acgcgtgatg aatcccgatc cggtctgcaa gcagtccctg
     5221 taactcggga ataattgcgc cgcccacaat cgcggtgatc agcaaacctg aagcctcgcc
     5281 ggtcagaggg cccatctccg ccacgcccag cgtaaaaatc gtggggaact ggatggagtc
     5341 aaaaagaccc accagtaaaa tgctccacac cgccagcccg ccgctggtca acatcgagac
     5401 gacggtaagc gtcatcgcgc acagcgcaaa cacgctgagc atgcggccgg gactcacctt
     5461 ctgctgcaac cccgagccga tgaatcggcc caccatcagt ccgccccagt agagcggcac
     5521 ccaggttccg atgatcttcg ttgccagctg cagaatcgcc gccgagcccg catggaacgg
     5581 tccgcctcct ggcgttgtgg ccaggccgcc gatctccggt tgcgcgaagt agttaatcag
     5641 gaaactcccg atcgaaacct cagcgcccac ataggcgaaa atcgcaagcg ccccaaagac
     5701 cacatgcgag tgcgaccaaa tgctgccctt ttccttatgc agctcgctcg ggcggtagtc
     5761 cttcgtcgtc tcgatgcggg gaaatttgta catcgcgatc gcgaggccga gcaataccag
     5821 cgcgagcgca atccccacat aaggaacatt gaccgtggac gcttcgtgaa tctgataagc
     5881 gcggtgcacc tccggcgaca tcttcgtgac gtcctgcacc gaaccggccg tccgcaggat
     5941 cacgaaggct ccgaagatcg gtcccagcac ggttcccagc gaattgaacg cctgcgttaa
     6001 gttcagccgg ctggacgcgg tgcgcggcgg tcccagcacc gtgacatatg gattcgccgc
     6061 cacctgaagc gccgtgatgc ccgcggccag cacgatcagc gcgccaagga acagcgggta
     6121 cgacggcacc ttggctgccg ggacaaacag caacgagccg acgcccatgg tgagcagccc
     6181 ggccaccatc gtcttcttgt agccgatcca ctccaccacc ttccccgatg gaatccccag
     6241 caacgcgtag gttgtgaaga aggcaaattg caccagcatc acctgtgcgt aattcagctc
     6301 gaaaatcgat ttcagatggg gaatcaggat gtcgttgagc gaggtgagaa agccccacat
     6361 gaaaaacagc gtcgtgacca tcgccagagc gctgcggtag ctggttcctt cgccgccggg
     6421 tgcaacctga atcgtttgac tgctgctggt gccggagatt gccatggttt gctcgattct
     6481 cgtcctgctc gttcctgtct ggtcttcgtt agatgtccgg atgcggacag tggagggata
     6541 ccgcacaacc agaaaatggg ccctcgccga tcatatattc ggacgagcat gcccaagccc
     6601 tgacgaaggt accggaatcc ggtaagaaac cctttctgtt gtgaaacttt gcacccacct
     6661 gctcgtcaga tcagtgaggt ttcccgcgag ataatggaat gaagatgagt tccccacgac
     6721 atttgcgcct cgccgtagcg gcgctctgct gcatcttcgc catggcggcc gcttccagtg
     6781 ccgctgcgca ggcacccgcg tcgaataagg tcatcgggac cgttacggcc gttagcggaa
     6841 cctccgtttc ggtaaagcag gattccggct ccgacgcgac ggtgaacgtt cctgagacgg
     6901 ccaatgtggt tcgaaccgag ccgggttcga agacgctggc cggcgccacg aagatcgccc
     6961 tcgcggacat cgaagcaggg gatcgcgtgc tgatattggt ttccggcgac ccgcctgtgg
     7021 ccaaagtggt cgtcgtcaac aaaaagagcg acatcgccgc caaacagcag caggaacagg
     7081 ccgactgggc acgccggggg gtgggcgggc tggtgaaggc agtggatggc ggcgcgggaa
     7141 ctgtaacgat cgcggcgaca aatcgaaccg tcacaattca cacgacagcc tcaacggtca
     7201 tccgacgtta cgctcccaat tctgtgaaat tcactgacgc gcagccctcg acgctggcag
     7261 ccattcagcc gggcgatcag gtggaagccc ggggagatcg cagcggagac gatgtgaagg
     7321 ccgacgagat cgtttcgggg tcgttccgca acattgccgg taccgtttcc tcaattgact
     7381 cggccgccaa ttcattcaca gtcaaagatc tggtgacgaa gaagagcgtc acgatcaaga
     7441 cgactcctga ctccgacctg cgcaaactcg atccgcagat ggcgcagatg gtcgcaatgc
     7501 ggctgcgcgg cgggggcaac ggtggcggac agacccagca aagctccgga gaacatgctg
     7561 ctggtccggg cggcagccag gtccgtcagg gcggcggcgg cccggccaat acgggcggac
     7621 agggcgtggg attctcccgg gtcctgcagc attcacccga gattcatgtc tccgatctgc
     7681 acaagggcga tgcagtcatg attgttgcca cctccggcga tcccgattcg gcgaccgcga
     7741 ttcgccttct ggccggcgtc gagccgatgc tgcaggcctc agccagcgga agccagagca
     7801 tgttctcctc agcatggagc ggactcggtg gcggcggcgg cgatcagggc ggtggcgagg
     7861 gtacttccca gtagtgtgta gaaatcgatg ttcctcaggt gaactcgccc agccaaaaac
     7921 acgtctgtct tagtacgacc tggtttcatc acgggctgcc gaaaaccctc tgcagcgacc
     7981 gttaaaagag aaggaaaatc cgttgaaccc aagaattatg cattgcctga agtgtctgac
     8041 tctccggatt ggctatgcgg ggaccagctc ttgcccggtt ccctcgcgac aaatgggccg
     8101 ttggctgctg atcggaacgt tcgcaggact gctggcttgt gtgccgtcca gtccggcgca
     8161 ggaagccgtt gcacccacga ctccttctgt tccgcaggtc acatccgggg ctccgacgca
     8221 gaacgccggc ggcattcgcg gccaggtggc cgatccttcg ggcgcggtca tccctaacgc
     8281 caccatcgtt gtgcttgata cagccggcaa aacggtgggc aagacgacct ccgatgccgg
     8341 tggcgtctac gcggtccatg ggatcccccc tggaacctac tccgtctggg tgaccgccga
     8401 gggcttcgcc gcgtggcgcg tccccaatgt tgcggtggca gcggggcaaa tgaagaacgt
     8461 gaaccctgcg ctgcagatcc aggtgcagca acagcaggtc gaagtccagg cggaaaacac
     8521 cacgatcggc actgcggctg atgcaaatgc cagcgcccta gtcatcaagg gcagtgacct
     8581 caactcgctg tccgacgatc ctgacgagtt gcagaacgaa ctgcaggctc ttgccggtcc
     8641 ttccgcgggc cccaacgggg ggcagatcta tatcgatggc tttacgggtg gacagctacc
     8701 gccgaaatcg tccattcgcg aaatccgcgt caaccagaat cccttctctg cgcaatacga
     8761 ccggcttggt tacggccgca tcgagatctt taccaagcca ggcaccgata agctccatgg
     8821 gcaggctgag attgtgggca acacctctgc cttcaactca cagaacccga ttcttcagaa
     8881 cagaaccgag ccctcctact actcgtggat gctccacggt tcggtcggcg gccctatttc
     8941 gaaaagcgcc tcctattttg tcagcatgtt ctctcgcaag cagcaggatg aagacattct
     9001 ccaggcgatc gatccggcca cggtgcattt cgacagcaac ggtcttgcta ccggcagctc
     9061 gatcaacgat gccatcgaca atcccggctc gcgcctcaat gtcagtcctc gccttgacct
     9121 ccagctgggg aaggcgaata cgctcacggt gcgctacgaa ttcaaccggg gcgtcagcac
     9181 caacggcggg ctcaacgccc ttagcctgcc ctccaccgcg atcaatacgc atcaggagga
     9241 caataccctc cagctttccg acagcctgat cctgagcccg aaactcgtcg acgatatccg
     9301 ctttcagtac cgccgtattc gcaatagcca gaccgcagtc tcgtccctgc cctcttatac
     9361 cgtgcagcag tcgtttacgg ccggcggcag cgccgagcag tcggtgcagg atcacgaaaa
     9421 cgtcttcgaa ctgcagaact atttctcggg agctttcggc gcccactcgc tcaacttcgg
     9481 cgctcgcgtc cgcgcttacg acgacgtcaa cgcgagcacc agcggcagca acggttctta
     9541 cagctttacc gaccttgcgt ccttcgtcgg ctgcctcgat aatccgccgg cggcgagctg
     9601 ccatccgcgg caatacagtt acaactacat ccagaaccct gtcgccagcg cgatcctgtt
     9661 cgactccggc ctgttctatc aggacgactg gaagctcaat cctcgcgtca ccttcagcta
     9721 tggtctgcgt tgggagacgc agaaccggat ccacgacaag aatgactggg caccccgcgt
     9781 gacggtcgcc tatgctcttg gccgttcttc gggcaagcag cagcccaaaa ccgtcatccg
     9841 tgccgggtac ggctggtttt accagcggtt caccgtggct aacggatttg gcgcccaggt
     9901 accgtacatc gtcaacgcaa tccaccagaa cggcattaac cagcagcgtt ttatccagaa
     9961 cgcgtcgaac tccacgattg ccttcaatcc caacactccg acggtgatca cttccgggtc
    10021 gaccggcggc ggccccgcgg ctcccacgaa gtacaccatc gatccaaata tgaagaccgc
    10081 gaatgaaatg gaagccgcgg ctggggtaga ccggcagctc tcgaagttca tgaccgggaa
    10141 cgtcacctac gtctactccc agggcattca ccaatacttc acggacaacc tcagcgcggc
    10201 tgcatccgag gtcgcggccg ggggattcca ggacggggag tatccggaga cgcagccgga
    10261 tgagcctctg gaaaataatc tgcaatacca gtccggcggt ttttatcgtg agcaccagct
    10321 catggccacg atccgcgcca actaccgcgc cttcagcctc atgacgaact acacctggag
    10381 cgatgccaaa ggcgacactt caggcgtcgg taccgtgccg tcggtttcca gcaacccggg
    10441 actggactac gggcggacca gctttgccgt cgagcatcgg ttcatggttt tcggcaacgt
    10501 cacactgccc tggaaggtct ccgtttcgcc gatgctggtc gccaattccg gcaatccgtt
    10561 caacgtcaca accgggagcg accttaccgg caacaaccag ttcaacgcgc ggcccaccta
    10621 tgcagatccg tccaaatgtg acggcgtcaa cgagaactac atcagtgccg ggcacttcgg
    10681 ttgtcttgac atcaatcctt acaacaccgg caacgctggc gagaaaatca tcccctacgg
    10741 cgtcggcacc ggaccctctt ccgtcagcct caacatgcgt ctgaacaagt ccatcggcat
    10801 cgggcccagg ctaggtgaag gcgaacacgc agctggtggc gacggcgggg gctttcacgg
    10861 aggacctcgg ggactcggtg gtggcggttt cagcggatca cggggcggcc cggggcgcat
    10921 ggaagcggcg gtaaaccggc gatacacgct caacctcggt gtttgggcga cgaatgttct
    10981 caaccatgag aacctcggta cgccgaacgg tgtgctgact gccgggcaat cgcaaggaac
    11041 cggccggccc ggatcctact tcgatcagtc gcagtcttta gcgggaggat ttttcggacc
    11101 ctcaacgtcg ggcaaccgga gcgtcttcct ccaggcgagc ttcagcttct agaggcggca
    11161 ggcggcagcc gacggttggt tctggcagcc gcctcattcc ggctccctgc cgctaagatg
    11221 gtagggacca tgcctaatcc atcgctcggc tttgcaacgc gcgccattca cgacgggcag
    11281 gcgcctgacc cccgaaccgg ggccgtcacc gtgccccttt atctgacgtc cacctacgaa
    11341 ctccagggca ttgagcaaca ccgcggctgg gaatattccc gcgtttcgaa tccgacgcgc
    11401 gacgcagtcg aagccagtct cgcttccctc gaagggggga cgagcgggca cgcctttggc
    11461 agcgggatgg cggccatcgc cgcgctcgtt accatgctca aggtcggcga ccatgtcatt
    11521 tgtgggcaca atgtctacgg cggcacgacg cgcctgttca cacaggtcat cccgcatcac
    11581 ggcatcgagt tcagctttgt ggatacatcg aacgccgaga acgttcgcca ggcgatgcgg
    11641 ccgacgacga agctggtcca cattgaaacg ccgaccaacc cgctgatgat gctgacggat
    11701 attcgcgcgg tggcggcgat ttgccatgag cagggcattc cgctgagcgt cgataacacg
    11761 ttcatgtcgc cgtattttca gcggccgctg gagctgggcg cggatgtggt gatgcattcg
    11821 acgacgaagt tcctcaacgg gcactccgac gggctgggcg gcattctggt cacgacgcgc
    11881 agcgatctgg cggagcagtt tcggtttgtg cagaagtgca cgggaggcat cctttcgccg
    11941 tttgaggcct ggttgattct gcgcggcgtc aagacactcg ctgtacgcat gcgccagcac
    12001 gacgccaacg ggcgcgctgt cgccgaatat ctttcgaagc atccgaaggt gagcaatgtt
    12061 ctctatcccg ggttgacgtc gcacccccag catgagctgg cgaagaggca gatgtccggg
    12121 ttcggatcca tgatcgcctt tgaggtcgg
//
LOCUS       DASZYN010000011         4641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_129065, whole genome shotgun sequence.
ACCESSION   DASZYN010000011 DASZYN010000000
VERSION     DASZYN010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129065"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1484)
                     /locus_tag="VHE33_00265"
     CDS             complement(<1..1484)
                     /locus_tag="VHE33_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT95906.1"
                     /translation="MGEFLRRLHYLIHRRRLDAELESDMEFHREMAARAGNRNFGNTL
                     RLREQAWESWGWTWLDRLLQDLRFGLRILGRSPGFTLVAVLVLGIGVGVNVSAFSLFN
                     MVVLKPLPVPEADRLVRLERRSPEQYSNLMSWPSCLFYQEHAHTLSAAMAVLGVPPME
                     IDNDEHLTSASFVTPNYFVELGTRPAVGRVLDPGNDGSAQDAPAVVLSYGLWQRRFGG
                     DPNVVGRVIHLNGSPVTIAGVTPFSFASLGGQNPDLWLPIAQQPYIIAHSTVLKDWAQ
                     SSVQMWGKLAPGVSITAAAQEMLALTNQLRHEHPEAVWDQEGIQISPGGHLQVLQPKM
                     IRVAVMVGMLALLILIVACANVGGLMAARAVSRQHEIGIRIAVGAGRWRIFRQLCTES
                     LLLGAVGTVAGVGLSWGVLTVALAKLDAPKWLSARPDWRVLLFAAGVTVVTTLFFGLM
                     PALQIARQRQQKTFARQILVGMQIAASSVLLIVAALLVHAAQHAL"
     gene            complement(1513..1848)
                     /locus_tag="VHE33_00270"
     CDS             complement(1513..1848)
                     /locus_tag="VHE33_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVT95907.1"
                     /translation="MTFREDSLSPAGALPMLILRVLQSEPLHGYAIAQRIHLVSREVL
                     AVEEGFLYPTLQKMLRKGWVQAEWGISETNRKVRFYRLTRQGKVQLEKELASYGRITQ
                     GIQDVLRTA"
     gene            2057..3070
                     /locus_tag="VHE33_00275"
     CDS             2057..3070
                     /locus_tag="VHE33_00275"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HVT95908.1"
                     /translation="MANFEKAPAEVQEILSQPIQQLGLKLEGSPLERFVQQLYKELAA
                     KGLHKFRPRCYLTDEWGCPNMEPVIGIPFYLADPKLQRLESEMNDIEDARQIMMYLRH
                     EAGHAFNYAYALYKTQEWRDLFGPFRRPYRDNYKPVPFSRQFVRHMEGWYAQKHPDED
                     FAETFAVWMTPRSNWRQRYKGWGALKKLLYMDRIGRKLRDADPIVAHGDTDITVDEMN
                     VTVGEFYQRALDQQLVPGELPFDTDLRDIFSVSKRRRKNVRPAVDLLRENRKVLVDKV
                     NYWTGVQRPLVKKLIESIEAKVADLGLRADIKCEREYLTEVSVYATALAMNYITRGKF
                     ILP"
     gene            3118..4164
                     /locus_tag="VHE33_00280"
     CDS             3118..4164
                     /locus_tag="VHE33_00280"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HVT95909.1"
                     /translation="MANLKITILHDVWEEGPPEPEPEPVRVPRTSKGKRPRKKKKDPL
                     HDREEIFEALTKLGHEPSYHLLDGRNQSLLALARCGADLVFNLTESYAGDDTMDKNIA
                     SYLELLQLPYTGSGPEALYLAQDKSIAKKIFDFHKIKTPDFAVSYKGRTDHSHDIEFP
                     LIVKPVSEDGSIGIDAGSLVDSVKELMERIHYIHEEFDSPALIEEYIEGREIYASILG
                     NENPQVLPLIELDLSKLPKGTPRIACQDVKFDHDTEAYKVTKSAPVEDLDEEILKKVQ
                     DTGLAAYRALGLRDYGRIDMRLNKKGQVYVIEANPNPWLASAAEFTMAAKKAGYSYTD
                     MIEKMIGLAQARFF"
BASE COUNT         1032 a         1559 c         1207 g          843 t
ORIGIN      
        1 agcgcgtgct gtgctgcgtg aaccagcagc gccgccacga tcagcaacac cgaactggcc
       61 gcaatctgca tgcccaccag gatctggcgt gcgaacgtct tctgttgccg ctgccgcgcg
      121 atctgcagag ccggcatgag gccgaaaaac agcgtggtca ccacggtgac gcccgccgcg
      181 aacagcagca ctcgccagtc cggtcgcgcg ctcagccatt tcggcgcgtc cagtttcgcg
      241 agcgcaaccg tcaatacccc ccaggataag cccactcccg caaccgtgcc caccgcgccc
      301 agaagcaggc tctccgtgca gagctgccga aaaatgcgcc accgtcccgc gcccaccgca
      361 atgcgtatcc cgatctcgtg ttgccggctt accgctcgag ccgccatcag tcccccaaca
      421 ttcgcgcaag ccacaatcaa aatcagcaga gccagcatcc cgaccatgac agccacacga
      481 atcatcttcg gttgcagcac ctgcaggtgt cctcctggag agatctgaat cccctcctga
      541 tcccataccg cctccggatg ctcgtgccgc agttggttgg tgagtgcgag catctcctgt
      601 gcggcagcgg ttatgctcac acccggcgcc agcttgcccc acatctgcac gctgctctgg
      661 gcccagtcct taaggacggt gctgtgggcg atgatgtaag gctgctgggc gatgggaagc
      721 cagagatccg gattctgacc gcccaggctg gcgaagctaa acggcgtgac tccggcgatg
      781 gtcacggggc tgccgttcag atgaatcaca cgccccacca cattcggatc gccgccgaat
      841 cggcgctgcc agaggccata gctcaggaca accgcgggcg catcctgggc actgccgtcg
      901 ttgccagggt ccagcactcg ccccaccgcc ggtcgtgttc ctagctcgac gaagtaattc
      961 ggagtcacaa aagatgcgct cgtcaggtgc tcatcgttgt caatctccat cggcggcacc
     1021 cccagcaccg ccatggcggc ggagagcgta tgagcatgct cctgatagaa gagacacgac
     1081 ggccaggaca tcaggttcga atactgctcc ggcgagcgac gctccagacg gacaaggcga
     1141 tccgcctcag gtactggaag gggcttcagc acaaccatgt tgaacaggct gaaggcggac
     1201 acgttgacgc ccaccccaat acccagcacc agcacagcga ccagagtgaa tccgggcgac
     1261 cgccccagga tcctcagccc gaaacgcaga tcctgtagaa gtcgatcgag ccacgtccag
     1321 ccccatgact cccaggcttg ttcgcgcaac cgcagcgtgt tgccgaaatt cctgttcccg
     1381 gcccgtgccg ccatctcgcg gtggaactcc atatcgctct cgagttcggc atcgagtcgc
     1441 cgtcgatgga tcaggtagtg gagccgtcgg agaaactcgc ccataagaaa cctcgttagc
     1501 cggtgttgat cactatgccg tgcgaagcac gtcctggatg ccctgggtga tccgtccata
     1561 gctggccagt tccttttcca gctggacttt gccctgccgc gtcaggcggt aaaagcgaac
     1621 cttacggttg gtttccgaga tcccccattc tgcctggacc catccctttc gcagcatttt
     1681 ctggagcgtc ggatagagaa acccctcctc taccgcaagg acctctcggg aaaccagatg
     1741 aatccgttgc gcaatggcat acccgtgcag cggctccgac tgaagcacac ggagaatgag
     1801 catgggcaat gcccctgcgg gactgagcga gtcttccctg aaagtcatat gcctaaaatt
     1861 aatatgccta gcgatactag gtcaatgtct ctcttctacg cctatgggca ggttgatttt
     1921 ggaagaacgg aacgagccgc gctccaacac caccgggaga agtcctttct catgccccac
     1981 cctctttccc ctcctgcccc atcaggccgc actctctcgc cgccctctat cccccgctgt
     2041 gctacgcttc gcaccggtgg ccaatttcga aaaagcgccg gctgaagttc aggagatcct
     2101 ctcccagccg atccagcagc tcggtctgaa gcttgagggc tcgcccctcg aacgattcgt
     2161 ccagcagctc tacaaagagc tcgctgccaa aggtctccac aaatttcggc cccggtgcta
     2221 cctcaccgac gagtggggtt gccccaacat ggaacccgtc atcggcattc ccttctacct
     2281 cgccgacccc aagctgcagc gcctcgaatc cgagatgaac gacatcgagg acgcgcgcca
     2341 gatcatgatg tacctgcgcc acgaggccgg ccacgccttt aactacgcct acgcgctcta
     2401 caagacccag gaatggcgcg acctcttcgg acccttccgg cgcccttacc gtgacaacta
     2461 caagccggtc cccttctccc gccagttcgt ccgccacatg gaaggctggt acgcgcaaaa
     2521 gcatcccgat gaagacttcg cggaaacctt tgccgtctgg atgacccctc gctccaactg
     2581 gcggcaacgc tacaaaggct ggggcgcgct gaaaaagttg ctctacatgg atcgcatcgg
     2641 acggaaactc cgcgacgccg accccatcgt cgcgcatggc gataccgaca tcaccgtcga
     2701 cgaaatgaac gtcaccgttg gcgagttcta tcagcgcgcc ctcgatcagc agctcgtccc
     2761 gggcgaactt cccttcgaca ccgacctgcg cgacatcttc agcgtctcga agcggcgcag
     2821 aaaaaatgtt cgacccgccg tcgatcttct tcgcgagaat cgcaaggtcc tcgtcgacaa
     2881 agtgaattac tggaccggcg tgcagcgtcc gctcgtcaag aagctcatcg aatcgatcga
     2941 agcaaaagtt gcagaccttg gactccgcgc cgacataaaa tgtgaaagag aatacctgac
     3001 cgaggtttct gtctacgcga cggccctggc gatgaactac atcacccggg gcaagttcat
     3061 tctcccctga cgggcgttgc tcgtgacgta cgtcgtaacc aagagcgcag aggcatcatg
     3121 gcgaacctga aaatcacgat cctgcacgac gtatgggagg agggcccgcc agagcctgaa
     3181 ccggagccgg tccgagtgcc ccgcaccagc aaaggcaagc gccccaggaa gaaaaagaaa
     3241 gatcctcttc acgaccgcga agaaatcttc gaagcgctga ccaaacttgg ccacgagcct
     3301 tcctaccacc tcctcgacgg caggaatcag tcgctcctcg ccctcgcccg ttgcggcgcc
     3361 gatctcgtct tcaacctcac cgagtcctac gccggcgacg acacgatgga caagaacatc
     3421 gcttcgtatc tggagctcct tcagcttccc tataccggca gcggtcccga ggctctctat
     3481 ctcgcccagg acaagtccat cgccaaaaaa atcttcgact ttcacaagat caagacccct
     3541 gacttcgctg tctcttacaa agggcgcacc gaccactccc acgacatcga atttcctctt
     3601 atcgtcaagc cagtctccga agacggctcc atcggcatcg acgcgggttc tctggtcgac
     3661 agcgtgaagg aactcatgga gcgcatccac tacatccatg aggaattcga cagccctgcg
     3721 ctcatcgagg agtacatcga aggccgcgaa atctacgcct ccattctcgg caacgagaat
     3781 cctcaggtcc tccctctcat cgaactggat ctctccaaac ttcccaaagg cacgccccgc
     3841 atcgcctgcc aggacgtgaa attcgatcac gacaccgagg cctacaaggt cacaaaatcc
     3901 gctcccgtcg aagacctcga cgaagaaatc ctgaagaagg tgcaggacac cggcctcgcc
     3961 gcgtaccgcg ctctcggctt gcgcgattac ggccgcatcg acatgcgcct gaacaagaaa
     4021 ggccaggtct acgtcatcga agccaatccc aacccgtggc tcgccagcgc cgcagagttc
     4081 accatggccg ccaaaaaagc cggctactcc tacaccgaca tgattgagaa gatgatcggc
     4141 ctcgctcagg cccgcttctt ctagtgtggc gtttccgagg tttctttatc ctgtcgtccc
     4201 gaccgaaccc tgagcacagg gaaggggaag cggagggacc tgcttccctt cagcgggaag
     4261 atatttcgga aacaccacct tagtgccgcg tttctttacc ttgtcatccc gaccgaaccc
     4321 tgagcatagc gaaggggaag cggagggacc tgcttccctt cagcaggaag agatttcgga
     4381 aacaccacat taaatatcct ccggcagcgc cggaggcttt acggttgctg gcccctcaaa
     4441 ggggcctgat cgcaaccgta aaagcaaaag taacggcgac agcagaccaa ttacctgcta
     4501 ccgatgccga aaccgcaaag acgaatgcca actcccgaac cgtcaaactg gtggccagtt
     4561 gggattacca gaagctgaca ccctggatgg gtcagtgggt tcactcgatc gcgcaacgag
     4621 acgctctctg actgttcccc g
//
LOCUS       DASZYN010000012         5674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1092833, whole genome shotgun sequence.
ACCESSION   DASZYN010000012 DASZYN010000000
VERSION     DASZYN010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092833"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="VHE33_00285"
     CDS             <1..1343
                     /locus_tag="VHE33_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HVT95910.1"
                     /translation="LSFVERWSRWHRGLAWVLVVAILAVRLFRGLVVAELVSPKVLSS
                     GRIFIVVTVAGSLLWIANRGWYAHAVRAGRFVQLLLGFSMLWLLPELVWMALHPEPHE
                     TAGFSKPIAQPPQRRIVWILLDEASQDQIFDHVQPGIAYPNFDRFAQSAVHFTNVQPA
                     GYFTEKVIPSLFTGDIITGERSDLDGRLFVRTEKNHRWHLYPDHSTLFAEAKQEGWST
                     AAVGWYNPYCRTDAAELDHCYWTLTTPLPGRYDPDRTALQNAAAPVAKSLLRMVGRRI
                     QDPTPWQIHAADYRVLLQHAREEIADRGGFVFIHLPVPHPGGIYNRRTHQVGVDGSYL
                     DNLVLADETFGTLLQAIDATPLADRTTVIISSDHSWRVNLWRPTTDWRAEDTRVSANR
                     FDPRPLLLVRFPGEREGAKISSAFPLIDMHAMIQAMLAGRIRTAAELQVWTGQQ"
     gene            complement(1344..1754)
                     /locus_tag="VHE33_00290"
     CDS             complement(1344..1754)
                     /locus_tag="VHE33_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95911.1"
                     /translation="MKRPWAVVLVAVMECFAAAIAWTVSFSLFVPGTRLDRMWQLNMP
                     VYEAFATQARPVATLLLLVGGLAAAAGVGLLTRRLWAWLLSLAIFGINALGDLVSLLI
                     TRDWLHSLSGVVIDAIFLYLLLRPGVRSFFLTRR"
     gene            complement(1769..3367)
                     /locus_tag="VHE33_00295"
     CDS             complement(1769..3367)
                     /locus_tag="VHE33_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HVT95912.1"
                     /translation="MILRKVVRLLLLPFLVSLACAQTATPHLFFRVTLDPSMHPASGR
                     LFIFLEPGSGAKSVQANEFHPSATSIAAMEVSALKPGASIDIDVDGLTFPAPFSTLKS
                     GTYQAEAVLDVDHTYAYDGAEPGDIESDVVPLPDWTPGTGTEPTFVLSHVVEARAERP
                     LPLKPDEQDAAERATSEVDFVSPALTAFSGRETHVRGWVVLPPGYAEHAKEHYPTVYW
                     THGFGAKLATIHSFALMIAGRMNAGTMPPMIWIFLDESLATGTHEFADSVNNGPWGRA
                     LTAEFIPWIEAKYRMDAKPSGRFLQGHSSGGWATLQLQVNYPQIFGGTWSTSPDPSDF
                     HDFTGVDLYAAHANVYHRPDGTPYPIVRDHGQVQATFEELTRQEGVLGAYGGQIASFD
                     WVFSPRGPDGRPLKMFDRDTGDVDPKVVAYWHDHYDLAHIVETNWTTRGPALKGKIHV
                     VVGTADTFYLDGAAHKFEAVLDRLGADPHFTYRDNRTHFDLYEENGDRLAMMDEIAEQ
                     MYAVARPAAHWKRPGGAAGAILHP"
     gene            complement(3473..4363)
                     /locus_tag="VHE33_00300"
     CDS             complement(3473..4363)
                     /locus_tag="VHE33_00300"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HVT95913.1"
                     /translation="MPPIAEIPAETVRADAEALEQTRSAKLNLIFAVTLTIFHVLAVV
                     ALFFFSWSALAVFLVLWVLGQNVGIGMSYHRLLTHRGYITPKWVEYAMALCATLSLQG
                     GPIYWVAVHRMHHQLTDRPGDPHSPRDGKWWSHMGWILHGSLRNKNPRLVRYVPDLAN
                     DSFYVWLSRWHWVPLTVVGISLFFIGGWSWFLWGVFLRITLGLHATWLVNSATHLWGA
                     RRFDTRDDSRNNWWVALLTGGEGWHNNHHAHPVSASHGMAWYELDFNYWGIRLLGFFG
                     LAKRIKVLGPDGGPARILHP"
     gene            complement(4459..5490)
                     /locus_tag="VHE33_00305"
     CDS             complement(4459..5490)
                     /locus_tag="VHE33_00305"
                     /inference="COORDINATES: protein motif:HMM:NF025022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HVT95914.1"
                     /translation="MDLFALRLLFVLVFTLACWFFRPFGLSPWTAGGVGAAVSGLIIF
                     FELRVRALSLRRLFGAVIGSLLGIMGAFLFSLVLRNSLPEGHTRSLFQLSVLLLMTYI
                     GLVVGANKGDLLNLAALGGLFVGERANKRNAKVIDTSAVIDGRIADIAETGFLEGVLI
                     VPEFVLRELQMVADSSDSSKRQRGRRGLDVLQRMRGNAQITVQIAEEDYPHIKEVDLK
                     LIELARQMEAKIVTNDFNLNKVAQVRGVAVLNINDLANSLRPVVLPGEKMRVVVMKEG
                     KEFDQGVGYLDDGTMVVVDHARRLIGRSVEICVTSVLQTASGKMIFGKLDEASRPEAV
                     RATAEASNG"
     gene            complement(5442..>5674)
                     /locus_tag="VHE33_00310"
     CDS             complement(5442..>5674)
                     /locus_tag="VHE33_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95915.1"
                     /translation="LPHRSGSDVNPSFRVIHCENGGLGLRWRACVYSPLPGNAGGFLI
                     PLLFFFRVAGVRRRGPEWIYSLFVCSLSLSLP"
BASE COUNT         1179 a         1786 c         1674 g         1035 t
ORIGIN      
        1 ttctgagttt cgttgaacgc tggagccgct ggcatcgcgg ccttgcctgg gtgctcgtcg
       61 tcgccatcct ggcggtgcgc ctctttcgcg gcctcgttgt agcggagctt gtctcgccga
      121 aagtcctgag cagcggtcgt atctttatcg tcgtgacagt tgcgggctcg cttctgtgga
      181 tcgccaaccg cggatggtac gcgcatgccg tacgcgcagg acgattcgtc cagctattgc
      241 ttggattctc gatgctgtgg ctccttcctg agttggtgtg gatggcgttg catcccgaac
      301 ctcacgagac cgcaggcttc tcgaaaccga ttgcgcagcc gccgcagagg cgcatcgtct
      361 ggatcctcct cgatgaggcc tctcaggatc agattttcga tcatgtgcag ccaggtatcg
      421 cctatccgaa cttcgaccgc ttcgcgcaga gtgcggtcca cttcacgaac gtgcagccgg
      481 caggatactt caccgagaag gtgattcctt ccctgttcac cggcgacatc atcaccggcg
      541 agcgcagcga tctcgatgga cggctctttg tgcgcacgga aaaaaatcac cgctggcatc
      601 tctatcccga tcactcgacg ctcttcgccg aggcgaagca ggaaggctgg tccacagcgg
      661 ccgtgggctg gtacaacccg tactgccgca ccgacgcggc cgaactcgat cattgctact
      721 ggacgctgac cacgcctttg cccgggcgct acgaccctga tcgaacggca ctccagaatg
      781 cggctgctcc ggtggcgaag tctttgctcc gcatggtcgg ccggcggatt caggatccaa
      841 cgccgtggca aattcacgcg gccgactatc gcgtgctcct gcagcacgcg cgcgaggaaa
      901 ttgccgatcg gggaggcttc gtcttcattc acctgcctgt gccgcacccg ggtggcattt
      961 acaatcgccg cacgcatcag gtgggcgtcg acggcagcta tcttgacaac ctggtgctcg
     1021 cagacgaaac gttcgggaca ttgctgcagg ccatcgatgc gactccgctc gccgaccgca
     1081 ccaccgtcat catctcctcc gaccactcct ggcgtgtcaa tctgtggcgt cccacgaccg
     1141 actggcgtgc ggaagatacg cgcgtttctg ccaatcgctt cgatccgcgg ccgctgctcc
     1201 tggtgcgctt tcctggcgag cgcgaaggag cgaagatctc gagcgccttc ccgctgatcg
     1261 atatgcacgc gatgatccag gccatgcttg ccggccgcat caggaccgcc gcggaacttc
     1321 aggtctggac cggtcagcaa taactagcgg cgcgtgagga agaacgatcg caccccggga
     1381 cgcaacagca agtaaagaaa aattgcgtca ataacaaccc cggagaggct atggagccag
     1441 tcgcgggtga tcagcaggct gaccagatcc ccaagcgcat tgatgccgaa aatcgccagg
     1501 gaaagcagcc aggcccaaag gcgccgtgtc agcaggccca caccggccgc agccgcgagc
     1561 cctcccacca gcagcagaag cgtcgctacg ggtcgcgcct gagtcgcgaa cgcttcgtac
     1621 acgggcatgt tcagttgcca catccggtcc agccgggtcc cgggcacaaa cagcgagaag
     1681 ctaacagtcc aggcaatcgc cgctgcgaag cactccatca cggcaaccag aacgacggcc
     1741 cacggcctct tcatttcact tgtttgtgtc atgggtgcag gatcgccccc gccgcgcctc
     1801 caggccgttt ccagtgtgca gccggacgtg cgaccgcata catttgctcg gcgatctcgt
     1861 ccatcatcgc caggcgatcg ccgttctctt cgtaaagatc gaagtgcgtg cgattgtcgc
     1921 gataagtgaa gtgcggatcg gcgccaagac gatccagcac cgcttcaaat ttgtgcgcag
     1981 cgccgtccag ataaaacgta tccgccgtcc ccaccacaac gtggattttg cccttcaagg
     2041 caggtccacg cgtcgtccaa ttcgtttcaa caatatgtgc caggtcgtag tgatcgtgcc
     2101 agtacgcaac gaccttcgga tcgacatccc cggtgtcgcg gtcgaacatc ttcaatggac
     2161 gcccgtcggg cccgcgcggc gagaaaaccc agtcgaacga ggcaatctgc cctccataag
     2221 cgccgagcac gccttcctgc cgggttaatt cttcaaacgt cgcctgcacc tgcccatgat
     2281 cgcgcacgat ggggtaaggc gttccatccg ggcggtgata aacatttgcg tgcgccgcgt
     2341 aaagatccac gccggtaaag tcatggaaat cgctcgggtc gggtgaggtc gaccaggtgc
     2401 ctccgaagat ttgcggatag ttgacctgga gctgcagcgt cgcccatcct ccggaagaat
     2461 gtccctgcag gaagcgcccc gacggcttcg cgtccatccg gtacttcgcc tcaatccacg
     2521 gaatgaactc cgcagtgagc gcgcggcccc agggcccatt gttcaccgaa tcggcgaact
     2581 catgtgtccc ggtcgcaagc gattcatcaa ggaagatcca tatcataggg ggcattgtgc
     2641 cggcgttcat gcggccggca atcatcagtg caaaggaatg aatcgttgcg agctttgcgc
     2701 cgaagccgtg agtccagtag accgttggat agtgctcctt cgcatgctcg gcgtagcccg
     2761 gcggcaacac aacccatcct ctaacatgcg tttcgcgccc ggaaaaggcc gtaagcgcgg
     2821 gactgacaaa gtcgacttcg gaggtcgccc tctctgccgc gtcctgttcg tcgggcttca
     2881 atggcagcgg gcgctccgca cgcgcttcca ccacgtgact gagcacgaac gttggttcgg
     2941 tgcctgtacc gggtgtccag tccggcaacg gaaccacatc gctctcgatg tcgcccggct
     3001 cggcgccgtc gtaggcgtag gtgtgatcca catcgagaac cgcctcggcc tggtacgttc
     3061 cggatttcag ggtggaaaac ggagcaggga acgtgaggcc gtcgacgtcg atgtcgattg
     3121 aggcgccagg cttcagagca gagacttcca tggccgcaat cgatgtcgca gaaggatgaa
     3181 actcgttcgc ctggacgctc ttcgccccgc tccccggctc cagaaaaatg aagagccgcc
     3241 ctgaggccgg atgcatgctc ggatccagcg tgacgcggaa gaacagatgc ggggttgcgg
     3301 tctgcgcgca ggcgagggaa accagaaacg gcaggagaag caggcgtacg actttcctga
     3361 ggatcatccc cgcgagtcta tcagtgaggc ggaaaccgtg ggaacacctc aggtcacaca
     3421 gaacgcaatc ccgaatctct attgcctgct ggctattggc tattccctgc ttttatggat
     3481 ggagaatgcg cgccggacca ccgtcaggac cgaggacctt gatgcgtttt gccagcccga
     3541 agaacccaag caggcgaatc ccccagtagt tgaagtccag ctcgtaccat gccatgccat
     3601 ggctcgccga taccggatgc gcatgatggt tgttgtgcca gccttcgccg ccggtcagca
     3661 gcgcaaccca ccagttgttg cgcgagtcat cgcgtgtgtc gaagcgccgc gctccccaca
     3721 ggtgcgtggc ggagttgacc agccaggtgg catgcaggcc gagggtgata cgcagaaaaa
     3781 cgccccacaa aaaccatgac catccgccaa tgaagaagag cgagatgccg accaccgtga
     3841 gcggcaccca gtgccagcgg ctcagccata catagaacga gtcgttcgca aggtcaggga
     3901 cgtagcgcac caggcgcgga ttcttgttgc ggagcgatcc gtgcaggatc cagcccatgt
     3961 gcgaccacca tttgccatcg cgcggggagt gcggatcgcc ggggcggtcg gtgagctggt
     4021 gatgcatgcg atgcactgca acccagtaga tcggcccgcc ctgcagcgac agcgttgcac
     4081 aaagcgccat ggcgtattcc acccacttcg gcgtgatgta gccgcgatgg gtgagcagcc
     4141 ggtgatagct cattccgatg ccgacatttt gtccgagaac ccacagcacc agaaaaaccg
     4201 ccagggcgct ccagctgaag aagaacagcg ccacgaccgc gagcacgtga aagatcgtca
     4261 gggtgacggc gaagatcagg tttagcttcg cggagcgggt ctgctccagc gcttcggcgt
     4321 cggcgcgcac ggtttcagcg ggaatctcag caataggcgg catggggaca aaaatacagc
     4381 ttcactttaa tggcactgcc gggcaagtga ctgtaagagt tgcgtaatct cgacgggcac
     4441 gcaccggccc ttggatcgtt agccgttcga ggcctccgct gtggcgcgca ccgcttccgg
     4501 gcgcgatgcc tcgtccagct tcccgaagat catcttgccc gacgcggtct gcagcaccga
     4561 tgtcacgcaa atttccaccg atctgccgat caggcgccgt gcgtgatcga ccaccaccat
     4621 ggtgccgtcg tcaagatagc cgaccccctg atcgaactcc ttgccttctt tcatgacgac
     4681 gacgcgcatc ttctcgccag gcagcaccac cgggcgcaga gagttcgcca ggtcattgat
     4741 gttgagcacc gccactccgc gcacctgcgc caccttattc aggttgaagt cgttggtcac
     4801 aatcttggct tccatttgcc gggccagctc gatgagcttc agatccactt ctttgatgtg
     4861 cgggtagtcc tcttcggcaa tctgcaccgt gatctgcgcg ttgccacgca tgcgctgcag
     4921 tacatccagc ccgcgccgtc cgcgctggcg cttcgaggag tctgaggagt ccgcgaccat
     4981 ctgcagctcg cgaaggacaa attccgggac gatcagcaca ccttcgagaa aaccggtctc
     5041 cgcgatatct gcgatgcggc cgtcgatgac cgcgctggtg tcgatgacct tggcattgcg
     5101 cttgttcgcg cgttccccga caaagagtcc acccagcgca gcaagattca gcagatcgcc
     5161 cttgttcgcg cctaccacca gaccgatata cgtcatcagc agcagaacgc tgagctggaa
     5221 caggctgcgc gtatggccct ccggcaggct gttgcgcagc accagcgaga agaggaacgc
     5281 tcccatgatg cccagcaggc tgccaatgac ggcgccgaag agccgccgca ggctcagcgc
     5341 gcgaacccgc agctcaaaga agatgatgag gccggagaca gctgcgccaa cgccgcccgc
     5401 agtccacggt gaaaggccaa acgggcgaaa aaaccagcag gctaaggtaa agacaaggac
     5461 aaagagcaga cgaagagcga ataaatccat tcaggccccc gacgacgtac gcccgccacg
     5521 cggaagaaga ataaaagggg gatcagaaaa ccaccagcat tgccgggcaa aggggagtat
     5581 acgcaggccc gccagcgcag tccaaggcca ccattctcac aatggatgac acgaaaagag
     5641 ggattgacat cggacccgct gcggtgcggt aatg
//
LOCUS       DASZYN010000013         7868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1156772, whole genome shotgun sequence.
ACCESSION   DASZYN010000013 DASZYN010000000
VERSION     DASZYN010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156772"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..445)
                     /locus_tag="VHE33_00315"
     CDS             complement(11..445)
                     /locus_tag="VHE33_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT95916.1"
                     /translation="MESPSSPPPRQTLGYVALLVRDYDEAIAWFCGVLGFALLEDTPL
                     EGKRWVLVAPPGSRETRLLLARAATPDQKKAIGNQSGGRVFLFLHTDDFWRDYHALVG
                     KGVRFRRDPREETYGTVAVFEDLCGNLWDLLQLRSTNPPEVS"
     gene            577..1464
                     /gene="motA"
                     /locus_tag="VHE33_00320"
     CDS             577..1464
                     /gene="motA"
                     /locus_tag="VHE33_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HVT95917.1"
                     /translation="MFAIIGIVVVFGAVVGGYLMEHGHLGVLVQPAELLIIGGAGGGT
                     LLIANPLRILKKIMGGAMGIFGSTKFGKPRYLSTLRMMYELLQKARRGGMLTIESDVE
                     KPDESPIFQGYPEFVKNHHLRDFVCDTMRMVITGGVDAFDMDQMMDLDMEVQHHSEEE
                     PVTALSTMADSLPGLGIVAAVLGVVITMGALGGPAEEIGHKVAAALVGTFLGILLCYG
                     LVGPVSQNMAKAIAEEHAYLYVLRVLILAFIRGQPPVVAIEMGRRAIPAHVRPSFAEV
                     EEACRNQPAAAAATTDAAA"
     gene            1474..2511
                     /locus_tag="VHE33_00325"
     CDS             1474..2511
                     /locus_tag="VHE33_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HVT95918.1"
                     /translation="MAGRPIIVIKKKSAHGGHHGGAWKVAYADFVTAMMSLFIVLWLM
                     NTSEKVRQAVAGYFNDPRGRSTMTGTDQSGSSENLALTKDNIPELKKKIEEAIRQMND
                     LKALQNQIQITITPEGLRIELLETKDGTFFDSGSATLNRNGRELLEMLAGQLGGLPNR
                     ISIEGHTDAAPYASKDGYGNWDLSTDRANAARRVMQVSGLRSSQVSQVRGYADQKLRV
                     PEDPLDPSNRRISLIVQYLTAEAPAVPADGKARPAEGGEGAKPQQEGPSIAIPASGGG
                     AQKKETSSGAPSASEAKPDSEKPAEGKTQDPAGAAGQSPAKPAPGGKPSAARVATANM
                     VSRLWHKVRRK"
     gene            2679..3377
                     /locus_tag="VHE33_00330"
     CDS             2679..3377
                     /locus_tag="VHE33_00330"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HVT95919.1"
                     /translation="MSEETSDIDLLHDIGSRMAAADPFHEVLQRVIVMVTAAVRCDSC
                     FIYVLEKEKLVLRASKNPHEDVVDTMQIDVGQGVTGWVAEHRQTVALPAKAWADPRFA
                     RFPALPEDRFEAFLAVPILCRGKLVGAINMQHREPHHHSKREIKLITMIGYLVGAEVE
                     LARMEYEKSQLADQLETRKVMERAKGLLQRELGIDEEAAYLMLQKQSRQKRRPLREIA
                     EAIILMDDLRKGKK"
     gene            3629..6067
                     /locus_tag="VHE33_00335"
     CDS             3629..6067
                     /locus_tag="VHE33_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT95920.1"
                     /translation="MLTDLRFALRQMLKSPAFTVTAIVTLALGIGANTAIFSVVNAVL
                     RHPAGVDHPERIAVMHVRYTQFTLDVPFVSVPTFAKANSMRDEVEAAAIENGTSFNIT
                     RDGQAENIPAARVSSRWFDVYGARPILGRTFTSDEDQPNAGPVAVLSYGMWQRVFGGR
                     QDVVGQTLLLDQKPYRVLGVMRSDFAWPRKADVWVPMALPPAAFNPENLFNESYYVAA
                     RLREGVAVEKFSASIAAAMWATLQGSERGTSFAKSSGWSVYATPLTDFAAGPLRTPLY
                     VLCGVVALVLLIAAANVAGLLLARASARSREFAIRTALGASASRMVRQMLVETLLLAG
                     VAAIVGVIAGPFIGALLLHLVPHDLARGFVVHTDPAVLGFTAAVAILAAVIAGLGPAI
                     GLMRSRGRLALHEGGRGGTASAEKQRMRSAFVIAEVAAAFFLLAGTGLFLTSLEKLQK
                     VDPGFNPRGVMAAMVPYSGQELTKNQPRQATFVSSVVSSLAAEPGVQAAAAVSPLPFD
                     PNGGGSCSFSIVGRPEAPNDPGPHSQLSLASPDYLKVMQIPLIAGRWIEPGDVAGSQP
                     VVVIDQRLAKKYFAGQNPVGQHMSFQCGGDLKNPATVIGVVATVRLSSLEEDTSDGMR
                     YFPFAQGKGFKADFLVRTNGNPNQMAPALKRAVAAVDSSQAVSILVPVETLVSDSLAG
                     RRLIVRMLAVFGGLALLLAIVGIYGLISYVTAQRTGEVGVRMALGAQRIDVLRLILGH
                     ALVLVAIGLGIGAVLSATASAILRRVFTDFGGGIFSSLALAAVALLVVGVLAGLIPAL
                     RAASLDPVKALRTE"
     gene            6128..>7868
                     /locus_tag="VHE33_00340"
     CDS             6128..>7868
                     /locus_tag="VHE33_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT95921.1"
                     /translation="MLGDLKYAWRQLKRSPGFAVTAVVTLALGIGATTAIFTMFDQVM
                     LRMLPVERPQELVRLNWHGSFSGSMSAFGGDVGDYYSYPMYKDLRDKNQVFSGMLAAV
                     PTEAGVSWQNQAENERIEVVTGNYFQLLGLKPAAGRLLTTGDETAKDANAVVVLAYDY
                     WRTRFGGSRDVIGQALMINSHPFTIVGVAPENFRTAIGGYRPRVFVPVTMEAIVSPWR
                     AKLHNLDNHQSLWLTVVARLKPGVTTQQAQASLTPLWHSLRAQELTLYPHASASFKER
                     FVDKSTIKVLDDSRGFNPQRLDLGTPLIILLSMAGLLIAMCTLNVATLLLLRSAARVR
                     EMSMRYALGAKSSRIVRQLLIEGGLLGLAGVTGGLALSPVLARILVRLMTTAQPGREP
                     YSSAMDPRVLLFSVGLAFVISILFSIAPALHFLKPDLAGALRQSTGTASRTSQRFRKA
                     AVGVQIAISVLLLGGAGLFVRTLTNLHNQPVGFDTRNLINFGVDPQEAGYGEERTPQV
                     IKGVMERLAQVPRVQSVAGTTDPELEGNSSFEGYVIQGHPAREGENNDFETAWVTARY
                     FETLRLPLLVGREF"
BASE COUNT         1467 a         2198 c         2762 g         1441 t
ORIGIN      
        1 ggtgtccgga tcaggacact tccggagggt tggtcgagcg taactggagc aggtcccaca
       61 ggtttccgca gaggtcctca aagacggcga cggtcccgta ggtttcctcg cgcggatcgc
      121 gtcgaaagcg aacgcccttt ccgaccaggg cgtggtagtc gcgccagaag tcgtcggtgt
      181 gcaggaagag aaagacgcgg ccgccggact gatttccgat tgcttttttc tgatcgggag
      241 tcgccgcgcg ggcgaggagg aggcgcgttt cgcgggagcc gggcggggcg accaggaccc
      301 agcgcttgcc ctcgagcggg gtgtcttcaa ggagcgcgaa gccgagaacg ccgcagaacc
      361 aggcgatggc ctcgtcgtag tcgcggacga gcagggcgac gtacccaagg gtctggcgcg
      421 gcgggggaga ggaagggctt tccacaacaa cagtatggct gttgaggggc ggaaattggc
      481 cgatgagggg agtggaagct cggagagagc ggcgccgagg gctgcaggtg cggcaggtgc
      541 acggggcagt gcgggcggga aggatcgtcg ggcagcatgt ttgcaatcat cggcattgtg
      601 gtggtctttg gggcggtggt gggcggctat ctgatggagc atgggcacct gggagtactg
      661 gtgcagccgg ccgaactgct gatcattggc ggagcgggcg ggggcacact gctgatcgcg
      721 aacccgctgc ggatcctgaa gaagatcatg ggcggggcga tgggcatctt cggctcgacg
      781 aaatttggaa aaccgcgcta tctctcgacg ctgcgcatga tgtacgaact gctgcagaag
      841 gcgcgccgcg gaggaatgct caccatcgag agcgatgtgg agaagccgga cgagagcccg
      901 atctttcagg gctatccgga gttcgtgaag aaccaccatt tgcgggactt cgtctgcgac
      961 acgatgcgca tggtgatcac ggggggcgtg gatgcgttcg acatggacca gatgatggat
     1021 cttgacatgg aagtgcagca ccacagcgag gaggagccgg tgacggcgct gtcgacgatg
     1081 gccgattcgc tgcctgggct ggggatcgtg gcagcggtgc tgggggtggt gatcacgatg
     1141 ggcgcgctgg gcggcccagc ggaagagatc ggccacaaag tggctgcagc gctggtggga
     1201 acgtttctgg gaattctgct gtgttacggg ctggtgggcc cggtgagcca gaacatggcc
     1261 aaggcaatcg ccgaagagca cgcatacctt tacgtgctgc gggtgctgat cctggcgttt
     1321 atccgcgggc agccgccggt ggtggccatc gaaatgggac gccgggcgat tccggcgcac
     1381 gtacggcctt cgtttgcgga agtggaagag gcatgccgga accagccggc cgccgccgcc
     1441 gcaaccaccg acgccgccgc gtaagaggag agcatggcgg gcagaccgat catcgtcatc
     1501 aagaagaagt ccgcccacgg tggccatcat ggcggcgcgt ggaaggtggc ctatgccgat
     1561 ttcgtgaccg cgatgatgtc gttgttcatc gtgctgtggc tgatgaacac gagcgagaag
     1621 gtgcgccagg cggtagcggg gtatttcaac gatccgcgag gcaggtccac catgacgggc
     1681 acggaccagt ccgggtcaag cgagaacctt gcgctgacga aggacaacat ccccgagttg
     1741 aagaagaaga tcgaggaggc catccggcag atgaacgatc tgaaagcgct gcagaaccag
     1801 attcagatca cgatcacgcc ggagggactg cgcatcgagt tgctggagac gaaggatggg
     1861 acgttcttcg attcaggcag cgccacgctg aacaggaatg ggcgcgaact gctggagatg
     1921 ctggcggggc agctgggtgg actgccgaat cggatctcga tcgagggaca caccgatgct
     1981 gcgccttatg cgtcgaagga cggatacggt aactgggatt tgtcgactga ccgcgccaat
     2041 gccgcgcggc gcgtgatgca ggtttcgggg ctgcgttcga gccaggtgtc gcaggtgcgc
     2101 ggctatgcgg atcagaagct gcgcgtgccg gaggatcccc tcgatccttc gaaccggcgg
     2161 atttcactca tcgtgcagta tcttacggcg gaggcaccgg cggttccggc cgacggcaag
     2221 gccagacctg cggaaggtgg ggagggagcc aaaccgcagc aggaggggcc atcgatcgcg
     2281 attcccgcgt ccggtggtgg ggcgcagaag aaggagacct cgtcgggcgc accttcggcg
     2341 agcgaggcga aaccggattc cgaaaaaccg gcggagggaa aaacccagga cccggccgga
     2401 gctgcggggc agagtcccgc gaagccagct cctgggggca aaccatcagc ggccagagtg
     2461 gctacggcca acatggtttc gcgcttgtgg cataaagtgc gcagaaagta gagctgctta
     2521 accctgccgc agggaccagg aaagcgcgcg agggctcatc ttgtgccact ataaagttgt
     2581 cagcccatct tgaatcgcac gcgaagcatc gcagaccccg tcgtcggcgc gcggcagtct
     2641 tctcagggga ggggaacgac gtggacgaac tggtgcgaat gtccgaagag accagcgata
     2701 tcgatctgct gcacgatatc ggcagccgca tggcggcggc cgatccattt catgaagtgc
     2761 tgcagcgcgt gatcgtgatg gtgacggccg cggtgcgctg cgactcgtgc ttcatctacg
     2821 ttctggaaaa ggagaagctg gtgctgcgcg cctccaagaa tccgcacgaa gacgtggtgg
     2881 acacgatgca gatcgatgtg ggccagggcg tgaccggatg ggtggccgaa cacaggcaaa
     2941 cggtggcgct gcccgcaaag gcgtgggccg atccgcgatt cgcgcgcttt cccgccttgc
     3001 cggaggatcg cttcgaggct tttctggcgg tgccgatcct gtgccggggc aagctggtgg
     3061 gcgcgatcaa catgcagcac cgcgagccgc accatcactc gaagcgcgag atcaagctga
     3121 tcacgatgat cggatatctg gtcggcgccg aggtcgagct ggcgcgtatg gagtatgaga
     3181 agtcgcagct ggccgatcaa ctggagacgc gtaaggtgat ggagcgcgcc aaaggcctcc
     3241 tgcagcgcga gctgggaatc gatgaagaag ccgcatacct gatgctgcag aagcagagcc
     3301 gtcaaaagcg ccggccgctg cgggaaattg cggaagcgat cattctgatg gacgatctgc
     3361 gcaagggcaa gaaataagcg ccggcagcag gcaatagcca atagccaata gccaatagcc
     3421 agtagggacc agcgggtcgc ctctgactca ttgccgaaaa cgtccaattc cgaacagtgc
     3481 atcgcgattg cggacaggtg atacgcccac tcgttgcgta aactgtctct tttcatggaa
     3541 tttgggcgag tgaagttggc agggcaactg ccgcaatcaa cattagccct gaaatcaccc
     3601 actccccacg ggcgccggaa aagaaaccat gcttaccgac cttcgctttg ccctgcggca
     3661 gatgctcaag tcgcccgcct tcactgtgac cgccatcgtg acccttgcgc tgggcatcgg
     3721 cgccaacacg gccatcttca gcgtggtgaa tgcggtgctg cggcatccgg cgggcgtcga
     3781 ccatccggaa cggatcgccg tcatgcacgt ccgctacacg cagttcacgc tcgacgttcc
     3841 ctttgtctcc gtgccgacgt ttgcaaaggc caattcgatg cgcgacgaag tcgaagcggc
     3901 ggccatcgag aacggcacga gcttcaacat cactcgcgac ggccaggcgg aaaatatccc
     3961 ggcagcgaga gtttcgtcgc gctggttcga tgtgtacggc gcccggccta ttctgggacg
     4021 gacgtttacc tctgatgaag atcagcccaa tgccgggccg gtggcggtcc tcagttatgg
     4081 catgtggcag cgggtttttg gcgggcgtca ggacgttgtc ggccagaccc tgctgctcga
     4141 ccagaagccg taccgcgtcc ttggggtgat gcgcagcgac ttcgcgtggc cgcgcaaggc
     4201 ggatgtctgg gtcccgatgg cactcccacc ggcggcgttc aacccggaaa acctcttcaa
     4261 cgagtcgtat tatgtggcag caaggttgcg cgaaggcgtc gcggtggaaa agttcagcgc
     4321 gtcgatcgcc gccgcgatgt gggcgactct gcagggaagc gagcgcggca catcgtttgc
     4381 aaaaagctcc ggttggtctg tctatgcgac gccgctgacg gattttgccg caggaccgct
     4441 gcggactccg ctctacgtgc tgtgtggtgt ggtggcgctg gtgctgctga tagcggccgc
     4501 caacgtagcg ggactgctgc tggcgcgggc atcggcgcgc agccgcgagt ttgccattcg
     4561 caccgcactg ggggcgagcg cctcacgcat ggtgcggcag atgctggtgg aaacgctgtt
     4621 gctggccgga gtggcggcga ttgtcggcgt gatcgccggg ccgttcatcg gcgccttgct
     4681 gctgcatctg gttccacacg acctcgcgcg cgggttcgtc gtgcacaccg atccggcagt
     4741 gctcgggttc acggcggcag tggccatcct ggcggcggtg atcgctggcc tggggccggc
     4801 catcggcctg atgcggtcgc gtgggcgcct tgccctgcac gagggcggac gcggcggcac
     4861 cgcgtcggcg gagaaacagc ggatgcgcag cgctttcgtg attgccgaag tggcggctgc
     4921 gttctttctg ctcgcgggaa cggggctgtt tctcacgagc ctcgaaaagc tgcagaaggt
     4981 ggatcccgga ttcaacccgc ggggcgtgat ggcggcgatg gtgccatatt cggggcagga
     5041 acttacaaag aaccagccac gtcaggcgac gttcgtgtcg agtgtggtga gtagtttggc
     5101 tgctgagcct ggggtgcagg ctgcggcagc ggtgtcgccg ttgcccttcg atccgaacgg
     5161 gggcgggtca tgctcgtttt caattgtggg gcggccggag gcgccgaacg atcccgggcc
     5221 gcacagtcag ttgtccctgg cgtcaccgga ctatctgaag gtgatgcaga ttccgctgat
     5281 cgcagggcgg tggattgagc cgggcgacgt ggcaggcagc caacccgtgg tggtcatcga
     5341 tcagcgcctc gccaaaaaat acttcgctgg gcaaaacccc gtggggcagc acatgtcgtt
     5401 ccagtgcggc ggcgacctga agaatccggc aaccgtgatc ggcgtcgtgg cgacggtgcg
     5461 gctcagttcg ctcgaggaag acaccagcga cggtatgcgc tattttcctt ttgcccaggg
     5521 taaaggcttc aaagctgatt ttctggtccg cacgaacggc aatcccaatc agatggctcc
     5581 tgctctgaaa cgcgcggtgg ccgccgtgga ttcgtcgcag gcggtgtcca tcctggttcc
     5641 ggttgagacg ctggtctccg attcgctggc cgggcgtcgc ctgatcgtgc ggatgctggc
     5701 ggttttcggc ggactggccc tgctgctggc cattgtggga atctatgggc tcatcagtta
     5761 cgtgacggcg cagcgcaccg gggaagtggg ggtgcgcatg gcactgggcg cgcagcggat
     5821 cgatgtattg cggctgatcc tgggtcatgc gctggtgctg gttgcgattg ggctgggcat
     5881 tggggcggtt ctgtctgcga cggcgtcggc gatcctgcgc cgggtcttca ccgatttcgg
     5941 cggcgggatc ttttcgtcat tggcactggc ggcggtagct ttgctcgtcg tgggagtatt
     6001 ggctggactg atcccggcgc tgcgcgcggc gagtttggac ccggtgaagg cactcagaac
     6061 cgaataaagg cagcgttcag cgttcaaaac cgcagctgag ttggaattgg cagcaaggag
     6121 agatcgaatg ttaggtgatc tgaaatatgc atggcggcag ctgaaaaggt cgccggggtt
     6181 cgccgttacg gcggtggtca ccctggcgct ggggattggc gcaacgacgg ccatcttcac
     6241 catgttcgat caggtgatgc tgcgcatgct cccggtggag cgtccccagg aactggtgcg
     6301 gctgaactgg cacggcagtt tttcgggctc catgagcgcc tttggcggtg acgtcggcga
     6361 ctactattcc tacccgatgt acaaagacct gcgcgacaag aatcaggtct tcagcggaat
     6421 gctggcggca gtcccgaccg aggcaggcgt gtcgtggcaa aaccaggcgg agaacgagcg
     6481 catcgaggtg gtcaccggga attacttcca gttgctgggg ctgaagccgg cggcggggcg
     6541 gctgttgacc acaggagacg agacggcgaa ggacgccaat gctgtggtgg tgctggctta
     6601 cgattactgg cggacgcgct ttggcggctc ccgcgacgtg atcggacagg cgctgatgat
     6661 caacagccac ccgtttacca tcgtgggcgt cgcgccggag aatttccgga cggcgatcgg
     6721 cggctaccgc ccgcgcgtgt ttgtgccggt gaccatggag gccatcgtct cgccgtggcg
     6781 ggcgaagctg cacaatctcg acaaccacca aagcctgtgg ctcacggttg tggcgcgcct
     6841 gaagccgggc gtgacaacgc agcaggcaca ggccagcctg acgccgctgt ggcactcgct
     6901 gcgggcgcag gagctaaccc tttatccgca cgcttccgcg agtttcaaag aacgattcgt
     6961 cgacaagtcg accatcaaag tgctcgatga ttcgcgcggg tttaacccgc agcggctcga
     7021 ccttgggacc ccgctgatca tcctgctcag catggcagga ctgctcatcg cgatgtgcac
     7081 gctcaatgta gcgacattac tgctgctgcg gtccgcggcg cgggtccgcg agatgtcgat
     7141 gcgctacgct ctgggggcga agagcagccg cattgtgcga caactgctga ttgaaggcgg
     7201 attgcttgga ctggccggag tgaccggcgg actggcgctg tctccggtgc tggcgcggat
     7261 cctggtgcgg ctgatgacca ctgcccaacc gggcagggaa ccctattcgt cggctatgga
     7321 cccacgggtc ctgctgtttt cggtggggct cgccttcgtg atcagcatcc tgttctccat
     7381 tgcgccggcg ctgcatttcc tcaagcccga tctggcgggt gcgcttcgcc aaagcaccgg
     7441 gacggcttcg cgaacctcgc agcgattccg caaggccgca gtgggcgtgc agatcgccat
     7501 cagcgtattg ctgttgggtg gagcgggcct ttttgtccgc actctgacca acctgcacaa
     7561 ccagccagtc gggttcgata cgaggaacct gatcaacttt ggcgtggatc ctcaggaagc
     7621 cggctacggt gaagagcgca cgccgcaggt catcaaaggc gtgatggagc ggctggcaca
     7681 ggttccacgc gtccaaagcg tggcaggcac gacagacccg gagctggaag gcaattcgag
     7741 tttcgagggc tatgtgatcc agggacatcc agcccgcgag ggcgagaaca acgacttcga
     7801 aacagcgtgg gtcacggctc gctattttga gactctgcgc ctgccgttgc tggtcggtcg
     7861 cgaattca
//
LOCUS       DASZYN010000014         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_129270, whole genome shotgun sequence.
ACCESSION   DASZYN010000014 DASZYN010000000
VERSION     DASZYN010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129270"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            635..>1590
                     /locus_tag="VHE33_00345"
     CDS             635..>1590
                     /locus_tag="VHE33_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HVT95922.1"
                     /translation="MAFSLPLITTAQPPNPPGPAGGHHGPPPKISADAAKADPNSVIP
                     VIIQYKHDPGWAQDSQVNHLGGATKDTLHSIHAKTALLPAGQLGTLASDPNVAYVSLD
                     RPVHPREAVSINAPDYTYQPINAPAAWSSGYVGTGIGVAVIDSGVTVVDDLQKTDNLT
                     LPGVTGPAGPPAPLPPTPGAHGRVLYSATFAPATKDGNDQFGHGTHVAGLIAGNGKKS
                     SGNQYSMTFYGIAPNANIIDLRALDANGQGTDSSVIAAIEQAIALKDKYNIRVINLSL
                     GRPIWESYTQDPLCQAVEQAWKAGITVVVAAGNDGRDLAANP"
BASE COUNT          339 a          481 c          458 g          312 t
ORIGIN      
        1 ccacctgtgg ttccgaagtt acgtccagct gcatggtacc gagtccacca ggtcattccc
       61 aatgcgtctt tagttgcaag atcgacccag ggggaaggcc gcaagccctg agttgtcatc
      121 gatctgtctc atttcggaac gcgcgcgacg tttcctttcg caaccgtggg ccaggacccg
      181 cctggtgacg acagtgtttg ggttcgtcgc cgcctaatgg cactacggcc gcaaagagga
      241 gggttcggca cttctgacga tcgagcacag atgcgtcgtc ggcgaggctg cgtcgtggca
      301 tcgattgcca aaaatgtagt gactacggtt acttattgcg gaaatgaatc gaaacattgc
      361 cgcgtttcga ctcagctcta ccaccagcgt aaccagttcg gacggtgttg ttcgtaattc
      421 cacaatggaa ggatggtggc tctgattcag tgaccgtact atcccatcag tcattcggag
      481 agcatgactc tccgaagaaa tcgcgaatcc agcaagccga gattcactga cgttaactgc
      541 acttgaagaa gcctcggttc ccagccgtgg agatgtgagg gatgcaatga tctcgcgttc
      601 gaaatcgcta aaacacttgc tgtggttgtc gtccatggca ttttctctgc ctttgatcac
      661 cactgcccaa ccgcctaatc cgccgggacc ggccggaggg caccacggtc cgccgcctaa
      721 gatttcggcg gacgctgcga aagccgatcc aaattccgta attcctgtca tcatccagta
      781 caagcacgat ccagggtggg cgcaggacag ccaggtgaat cacctcggcg gcgcaacgaa
      841 agataccctg cactccattc atgccaagac cgcgctgttg cccgcgggtc agctgggcac
      901 gctggcttcc gatccgaacg tcgcctacgt ttcgctcgat cgtcctgtgc atccgcgtga
      961 agcagtctcg attaatgcgc cggactacac gtatcagccg atcaatgcac cggctgcctg
     1021 gtccagcgga tatgtaggta ccggcatcgg cgtcgccgtc atcgacagcg gcgtaaccgt
     1081 ggtggatgat ctgcagaaga ccgataacct gacgcttccc ggagttacgg gaccggccgg
     1141 accgccggct ccgctgccgc cgacaccggg cgcgcatgga cgcgtgctct attcggcaac
     1201 cttcgcaccc gcgacgaagg acggcaacga tcagttcggg cacggaacgc acgtggcagg
     1261 tctgatcgcc ggtaatggca aaaaatccag cggcaaccag tactcgatga cgttctacgg
     1321 catcgctccg aacgccaaca tcatcgacct gcgcgcgctc gatgcaaacg gacagggtac
     1381 ggactcctcc gtgatcgcag ccatcgaaca ggcgatcgct ctgaaggaca agtacaacat
     1441 ccgcgtgatc aacctctcgc tgggccggcc gatctgggag agctatacgc aggatccgct
     1501 gtgccaggcg gtggagcagg cgtggaaggc gggcattacc gttgtcgtcg ccgccggcaa
     1561 cgacggccgc gatctggcag cgaacccgat
//
LOCUS       DASZYN010000015         7156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1156860, whole genome shotgun sequence.
ACCESSION   DASZYN010000015 DASZYN010000000
VERSION     DASZYN010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7156
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156860"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VHE33_00350"
     CDS             <1..913
                     /locus_tag="VHE33_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HVT95923.1"
                     /translation="DASSRVATMAETAGGTIAMSNINGDKLEVAVAIGTDPATTFSAI
                     VPAPPEVEEFLIAGFLRQQPVELVKCETVNLEVPAHAEIILEGYVRLDELRPEGPFGD
                     HTGFYTAAEDYPVFHLTCITHRRDPIYSATIVGKPPQEDAWMGKAVERIFLPLFRLTL
                     PEIVDINLPVEAVFHNLMIVSIRKSYAGHARKVMNGVWAMGQAMFTKCIIVVDEDCDV
                     QDLSEVTLRVANNIDPERDIQFTLGPIDSLDHASRLPNYGSKMGIDATRKWSAEGFTR
                     PWPKEIVMDTATKSRIDQLWKNLGLED"
     gene            960..1496
                     /locus_tag="VHE33_00355"
     CDS             960..1496
                     /locus_tag="VHE33_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95924.1"
                     /translation="MFGIGRKSLPLHIVAASMAQVALTPLENPSEEDLSIRQQAVEAG
                     MDPNRFTLEVIALQTFAMSAAINRERVEGRLKLEKAQPLVHEFLQEVHKRLVETTAYD
                     LLKLGLEPDDAFEVFLARAERYSKPGWGHGNPEDIPGFFAEFCGIADSLVLRQIGWRL
                     TFIRGDLSGDWLKSIKIV"
     gene            1701..2186
                     /locus_tag="VHE33_00360"
     CDS             1701..2186
                     /locus_tag="VHE33_00360"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HVT95925.1"
                     /translation="MFTLDGIRQFHRWTHLCLGVALDHLATLPIADYTRDVPGFGSSN
                     LRAQVIHNINCEAFWVHTLQGLPYTDEAPESLPTVADARAFEQRVTAQTLDYLSHLTE
                     QQLNAPATLRFPDGDTATRTPALILHHMLTHAFHHKGQIVAMCRLLGHPAPDTDLNNF
                     E"
     gene            complement(2246..2887)
                     /locus_tag="VHE33_00365"
     CDS             complement(2246..2887)
                     /locus_tag="VHE33_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95926.1"
                     /translation="MSSVLRLAATVGLIGGILATGRAQGGAGTGTTAVPKVLAPYTSC
                     VFPDGLRVVQTSSLGQGPMVRPIQTANGPENIDLDAGEQVVFSYPLTDDFANAKVELL
                     PADRYMALKKVLIDNLHFLEHQRGGPTQALALPAGLHGFEVHGNDVRKLEGNALGMYV
                     MFDDKAHVATTVYFLNQRVWQRKFQTMEEYGRLRDNFLRTYTGCVRQNQALER"
     gene            complement(3024..3863)
                     /locus_tag="VHE33_00370"
     CDS             complement(3024..3863)
                     /locus_tag="VHE33_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HVT95927.1"
                     /translation="MWLAGLVVLACGLAAWGQNYTPVTVVVTGPDGAAVAGAEIRVTP
                     GSPAGDKLIADEQGRAQLGMPAGEYMLRVSAQGFGVADKVVVVGEKAQTVTVALVAAP
                     EASGGTEVNGSAPMSPGAVPLETTQGASHAASGTRPAAATTLTIVGADGERGVFTPQT
                     LREYPQKTVKVMDRRTSQEKTYGGVPLMDLLAHLGVAKRAMGKALAKYVVATGSDGFE
                     AVLSLAEVDPELHGGTVLVADAEDGKPLDAKSGPFQLVVSEDKGPMRSVRNLVRIEVR
                     TAE"
     gene            4017..4676
                     /locus_tag="VHE33_00375"
     CDS             4017..4676
                     /locus_tag="VHE33_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HVT95928.1"
                     /translation="MARLQDQLDDITSQTRSLVQPERLAIGERAVEELFATRIEDRIL
                     PVGAQAPSFALPDFSGKIVRSTDLLALGPIVINFFRGRWCPYCVTELEAWRDLYPGLR
                     ERGTMVIGISPQTQRQSDFTAGQHQIPFPLLTDLGCAVANKFGLVWTAPDYLQRYYRG
                     ILLNIPFVNGDESWKLPVPATYVISPEGKVLFAEAHADFRVRPEPEDVLRSLPAFARS
                     E"
     gene            complement(4762..5574)
                     /gene="trmD"
                     /locus_tag="VHE33_00380"
     CDS             complement(4762..5574)
                     /gene="trmD"
                     /locus_tag="VHE33_00380"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:NF000648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HVT95929.1"
                     /translation="MRFDLITIFPEFFASILGHGVLRRGIAGGLVRVEVHDLRNFAHD
                     RHRTVDDRPFGGGEGMVLKPEPLAEAVESIGMRAKGERDGGRERVVLLSAQGRRFTQA
                     AARELAGLERLVLLCGRYEGVDERVHELLCDEELSIGDYVLSGGELAAAIVVDATARL
                     LPGVLGNEASSAYESFGVRETGSAGEEGGPPVAVHGSGGLLDYPHYTRPAEFRGMAVP
                     EVLARGDHAAIRRWRREKALEKTLRNRPDLLEGAELTEADRRVLEKLGWKKG"
     gene            complement(5752..6249)
                     /locus_tag="VHE33_00385"
     CDS             complement(5752..6249)
                     /locus_tag="VHE33_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95930.1"
                     /translation="MLVATRSQRRDFFFADFFAAFFALFFAEVFLAAFFADFFAAFFA
                     LFLAGAFFAAFFTAFFAAFFAGAFFAAFLAGAFFAGAFFAAAFFAGAFFAAAFFAGAF
                     FTGGGGGGGAGSPMPSSSSSISSSSEVVVETTISSSSSSSPKPFSSSSRSSSASGSNS
                     PSALA"
     gene            6426..>7156
                     /locus_tag="VHE33_00390"
     CDS             6426..>7156
                     /locus_tag="VHE33_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT95931.1"
                     /translation="MPTITTKDGTEIYYKDWGSGQPITFSHGWPLTADAWDAQMLFLG
                     QQGYRVIAHDRRSHGRSSQTWEGNDMNTYADDLATLFEALDLKKAIMIGHSTGGGEVV
                     RYIGRHGTQRVAKAVLISAVPPIMLKSEKNPGGLPMSVFDDIRASTAGDHAQFWKDLS
                     LPFYGYNRPGAKISEGVRDGFWLQSMMGGIKGLYDCIKAFSETDFNDDLKKIDVPTLV
                     MHGDDDQIVPFHDAGELTAKIVKGA"
BASE COUNT         1454 a         2722 c         1704 g         1276 t
ORIGIN      
        1 cgatgcatcg tcgcgggtag caacgatggc cgaaaccgcc ggcggcacca tcgccatgag
       61 taacatcaac ggtgacaaat tagaggtcgc cgtcgccatc ggcaccgatc ccgccaccac
      121 cttctccgcc atcgttccgg cgccccccga ggtcgaggag ttcctcatcg ccggcttcct
      181 ccgccagcag ccggttgagc tggtcaaatg cgagaccgtc aacctcgaag tccctgccca
      241 cgccgaaatc attctcgaag gctacgttcg cctcgacgag ttgcgccccg aaggcccctt
      301 cggcgaccac accggcttct acaccgcagc cgaggactac cccgtcttcc acctcacctg
      361 catcacgcat cgccgcgacc ccatctattc ggccaccatc gtcggcaagc cgccgcagga
      421 ggacgcgtgg atgggcaaag ccgtcgagcg catcttcctc ccgctcttcc gtctcaccct
      481 cccggaaatc gtcgacatca acctccccgt tgaggccgtc tttcacaacc tcatgatcgt
      541 ttcgatccgc aagagctacg caggccacgc gcgcaaggtc atgaacggcg tgtgggcgat
      601 gggccaggcc atgttcacga agtgcatcat cgtcgtcgac gaggactgcg acgtccagga
      661 cctctccgaa gtcaccctgc gcgtcgccaa caacatcgac cccgagcgcg acatccagtt
      721 caccctcggc ccgattgatt ctctcgatca cgcctcgcgc ctgcccaact acggcagcaa
      781 gatgggcatc gacgccaccc gcaagtggtc cgccgaaggc ttcacccgcc cctggcccaa
      841 agaaattgtg atggacaccg cgacaaaatc ccgcatcgat cagctctgga agaatctcgg
      901 cctcgaggac tagttagcga gtctcattcg gacgatctta cccggagtaa actcctttca
      961 tgtttggcat cgggcgcaag tcgcttccgc ttcatatagt ggccgcatca atggcgcagg
     1021 tggcacttac gccacttgag aatccgtctg aggaggacct cagtattcgg cagcaggccg
     1081 tcgaagctgg aatggatccg aaccgcttca ctcttgaagt tatcgccttg caaacgttcg
     1141 ccatgtctgc ggccatcaac cgagagagag ttgaaggccg gctgaagctt gagaaagcgc
     1201 aacccctagt gcacgaattt ttgcaggagg tccacaagcg actagtggag accacagcat
     1261 atgatctgct caaattgggg ttagaacctg atgatgcgtt cgaggtattt cttgcgcgcg
     1321 ccgaacggta ctccaaaccc ggatggggac acggcaaccc ggaagacatt cccggtttct
     1381 ttgcagagtt ctgtggtatc gcagactctc tcgttctccg ccaaattggt tggcgtctga
     1441 cattcatcag aggcgatctc agcggcgact ggttgaagag tattaagatt gtttaggtaa
     1501 cgaggaaacg agaatgagtg aatccgggat tggtcttctc tgctgcgaca cgcataacga
     1561 cgcgtacgtt gaggctcggc gaaggtctcg cgccgtcggt gtggactgga catctgagga
     1621 actcaaggcc ctccatgata tcgaagaaca tctcagggag ctaaaaggca ttgcgaacat
     1681 gagctaacct ccttcatcca atgttcaccc tcgacggaat ccgccagttc caccgctgga
     1741 cccacctctg cctcggcgtc gccctcgacc acctcgccac cctcccgatc gccgactaca
     1801 cccgcgacgt tcccggcttc ggctcctcga acctccgcgc ccaggtcatc cacaacatca
     1861 actgcgaggc cttctgggtc cacactcttc agggcctccc ttacaccgac gaagcccccg
     1921 agagcctccc caccgtcgcc gacgctcgcg ccttcgaaca acgcgtcacc gctcaaaccc
     1981 tcgactacct ctcccacctg accgaacagc agctcaacgc gcccgccacc cttcgtttcc
     2041 ccgacggcga caccgccacc cgcactcccg ccctcatcct ccaccacatg ctcacgcacg
     2101 ccttccatca caagggccaa atcgtggcga tgtgccggtt gctcggccat cccgcccccg
     2161 acaccgacct caacaatttc gaatagcatc gcgggaagca cgatgctgtt ttctgaacgc
     2221 tgaacgccga acgctgaacg ctgtcttacc tctccagcgc ctggttctgc cgcacgcatc
     2281 ccgtatacgt ccgcagaaag ttgtcccgca gccgcccgta ctcctccatt gtctggaact
     2341 tccgctgcca gacacgctga ttcaggaagt aaaccgtcgt cgccacatgc gccttgtcgt
     2401 cgaacatcac atacatcccc agcgcattgc cttccagctt ccgaacgtcg ttgccatgca
     2461 cctcaaaccc gtgcagcccc gccggcaacg ccagcgcctg cgtcggaccg ccccgttgat
     2521 gctcaaggaa atgcaggtta tcgatcagaa ccttcttcag cgccatgtac cgatccgccg
     2581 gcaataactc caccttcgca tttgcgaaat catccgtcag cggatacgag aacaccacct
     2641 gctccccagc atcgagatcg atattctccg gtccgttggc cgtctggatc ggacgcacca
     2701 tgggcccctg ccccagcgaa gatgtctgca ccacgcgcaa tccatccgga aatacgcagc
     2761 tcgtgtaggg agccagcacc ttcggcacag cggttgtgcc cgtaccggcg cctccttgcg
     2821 cccttcctgt cgccagaatt ccgccaatca aacccaccgt cgcagccaaa cgcaacacgg
     2881 aactcacatg taccattctg atcgcctgct cctgataacc atggtggata tcgcgtaaaa
     2941 cgcgatccag accgctgact cgctgacgcg ctgacgtgct gctcttttga acgccgaacg
     3001 ccgcgcgccg aaccctgttt ctgtcactcc gcggtcctca cttcgatccg caccaggttc
     3061 cgcacgctcc gcatcggccc cttgtcctcg ctcaccacca gctggaacgg ccccgacttc
     3121 gcatccagcg gcttgccgtc ctccgcatcg gccaccagca ccgttccgcc atgcagttcc
     3181 ggatccacct cggccagcga cagcaccgcc tcaaatccat ccgaccccgt cgccaccaca
     3241 tacttcgcca gcgccttccc catcgccctc ttcgccactc ccagatgcgc cagcaggtcc
     3301 atcagcggca cgccgccata ggtcttctcc tggctcgtcc gccggtccat caccttcacc
     3361 gtcttctgcg gatactccct cagcgtctgc ggcgtgaaca ccccgcgctc cccatcggct
     3421 cccacgatcg tcagcgtcgt cgctgcagcc ggccgcgtac cactcgcggc atgactcgcc
     3481 ccctgggtcg tctccagcgg caccgcaccc ggcgacatcg gagccgatcc attcacttcc
     3541 gtacccccgc ttgcctctgg agccgccacc aacgctaccg tcaccgtctg cgccttctcc
     3601 ccgacaacca cgaccttgtc agcaacacca aacccctgcg cagaaacccg cagcatgtac
     3661 tcgcccgccg gcatccccaa ctgcgcccgc ccctgctcat cggcgatcag cttgtcgcct
     3721 gccggactcc ccggcgtcac gcgaatctcc gctcccgcca ccgcggcccc gtccggaccc
     3781 gtcaccacca ccgtcaccgg cgtgtagttt tgcccccatg ccgccagccc gcacgccaga
     3841 accaccagcc ctgccaacca catccgaccc aatccccgaa cccgcataca cgtactctct
     3901 ttctctggtc cccataccgg cccagcacca caggatcacc gtttcgacct ccatcgtaac
     3961 cccagccctc agaaatcctc tgctgcaatt cgcctccccg catctacact gaggtcatgg
     4021 cgcgcctcca ggaccagctt gacgacatca cctcccagac ccgcagcctg gtccagcccg
     4081 agcgcctcgc catcggcgaa cgcgccgtcg aggagctctt cgccaccagg atcgaagacc
     4141 gcatcctccc cgtaggagcc caggccccct ccttcgccct gcccgacttc tccggcaaaa
     4201 ttgtccgctc caccgacctg ctcgccctcg gccccatcgt catcaacttc ttccgcggcc
     4261 gctggtgccc ctactgcgtc accgagctcg aagcatggcg cgacctctac cccggcctcc
     4321 gcgaacgcgg caccatggtc atcggcatct ctccccaaac ccagcgccag tccgacttca
     4381 ccgcgggcca gcaccagatt cccttcccgc tcctcaccga cctgggctgc gccgtcgcca
     4441 ataaattcgg cctcgtctgg accgctcccg actacctcca gcgctattac cggggcatcc
     4501 tcctcaacat ccccttcgtc aatggcgacg aaagctggaa gctcccggtc ccggccacct
     4561 acgtaatctc tcccgaagga aaagtcctct tcgccgaggc ccatgccgac ttccgcgttc
     4621 gccctgaacc ggaagacgtc ctccgctcac tccccgcctt cgcccgcagc gaatagttca
     4681 aaattgtcat cccgaccgaa gcgctcgcat cgcgagtgcg cagcggaggg acctgctttc
     4741 ctgcgccctg ctccgcattc cttatccctt cttccaccca agcttctcca gcacccgccg
     4801 atccgcttcc gtcagctccg ccccttccag cagatccggc cggttccgca gcgtcttctc
     4861 cagcgccttc tcccgccgcc accgccggat cgccgcatga tccccccgcg ccagcacctc
     4921 cggcaccgcc atcccccgaa attccgccgg ccgcgtgtag tgcggataat ccagcagccc
     4981 ccccgaccca tgcaccgcca ccggtggccc accctcctct cccgccgatc ccgtctccct
     5041 caccccaaag ctctcgtacg cgctcgaagc ctcattcccc agaacccccg gcagcagccg
     5101 cgccgtcgcg tccaccacaa tcgccgccgc cagctccccg ccactcagta catagtcccc
     5161 aatgctcagc tcctcatcgc aaagcagctc atgcacgcgc tcgtcgacgc cctcataccg
     5221 tccgcacagc agcaccagcc gctccagtcc cgccagctcc cgcgccgccg cctgcgtaaa
     5281 ccgccgcccc tgcgccgaga gcagcaccac ccgctcccgc cccccatccc gttccccttt
     5341 ggccctcatc ccgatcgact ccaccgcctc cgccagcggc tccggcttca gaaccattcc
     5401 ttcgcccccg ccaaaggggc ggtcgtccac cgtccgatgc cggtcgtgcg caaaattgcg
     5461 caggtcatgg acctccaccc tcaccagtcc cccggcaatc ccccgccgca gcaccccatg
     5521 ccccaggatg ctcgcgaaga attccggaaa aatcgtgatc agatcgaacc gcatcgattg
     5581 catcattctt tcatgtgcgc acacacgctc cgccttctgc gttggcaacc gactatgctg
     5641 ttgctataat ccgccgcgca ggagttttgc tgtttttctg gcatactcat acagcggtgc
     5701 aaactgaagc ggagcccgga ggacacatcg gatgcaggac gaatataagc tttacgcaag
     5761 cgcggacggc gaattcgagc cggaagcgga agacgatctc gaagagctcg aaaatggctt
     5821 cggcgatgac gaggaggaag aagagatcgt agtctctacc acgacctccg acgacgacga
     5881 gatggacgac gaggacgatg gcatgggcga acccgcgccc ccgccgccgc ctcctccggt
     5941 aaagaaagcc ccggcgaaaa aggctgctgc gaaaaaagct cccgcgaaga aagccgcagc
     6001 gaaaaaggcg cctgcaaaga aagcgcccgc caaaaaggct gcgaaaaaag cgcctgcaaa
     6061 gaaagcggca aagaaggcgg taaagaaagc agcaaaaaag gcccccgcca aaaagagcgc
     6121 aaaaaaggct gcaaagaagt cggcgaaaaa ggcagccaaa aagacttccg caaaaaagag
     6181 tgcaaagaag gcagcaaaga aatctgcaaa gaaaaagtcg cgccgctgac ttcgcgtcgc
     6241 caccagcatc aggggccgcc ggcaaaagcc gcgcggcccc tggttttttc cctcaactcg
     6301 ctgttcgcgc ttcctggcct cttaccttct gacagcctct cgctcaacgc ttctctgctt
     6361 tttcaccgtc cttttacccg gccccgaatc ttactctcaa cgtcctgaac gcaggagaaa
     6421 ccaacatgcc caccatcacc accaaagacg gaaccgagat ttactacaag gactggggct
     6481 ccggtcagcc catcaccttc tctcacggct ggcccctcac cgctgacgcc tgggatgccc
     6541 agatgctctt cctcggtcag cagggttatc gcgtcatcgc ccacgatcgc cgctcccacg
     6601 gccgttcctc ccagacctgg gaaggcaacg acatgaacac ctacgccgac gaccttgcca
     6661 ccctcttcga agcccttgat ctgaaaaaag ccatcatgat tgggcactcc accggcggcg
     6721 gcgaagtcgt ccgttacatc ggccgccatg gcactcaacg cgtggccaaa gctgtcctca
     6781 tcagtgctgt cccccccatc atgctcaagt ccgagaagaa ccccggcggc cttcccatga
     6841 gcgtctttga cgacatccgc gccagtaccg ccggcgacca cgcgcagttc tggaaggacc
     6901 tctcgctgcc tttctacggc tataaccggc ccggcgccaa aatctctgaa ggcgtgcgcg
     6961 atggtttttg gctccagagc atgatgggtg gcatcaaggg cctctatgac tgcatcaagg
     7021 ccttctctga aaccgacttc aacgacgatc tgaagaagat cgacgtccct accctcgtca
     7081 tgcacggcga tgacgatcag atcgtcccct ttcacgatgc tggcgaactc acggcaaaga
     7141 tcgtcaaagg cgccca
//
LOCUS       DASZYN010000016         3258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_707481, whole genome shotgun sequence.
ACCESSION   DASZYN010000016 DASZYN010000000
VERSION     DASZYN010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707481"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VHE33_00395"
     CDS             <1..387
                     /locus_tag="VHE33_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVT95932.1"
                     /translation="RARAEKLIEQLNLGHIRKTRGYALSGGERRRVEIARCLCIQPTF
                     ILLDEPFSGIDPIAVLDLQEIIFDLKASGIGVLITDHNVRETLSVTDRAYIINEGRIF
                     YHGTPDQLGNDPEVRRVYLGEGFSLG"
     gene            508..2295
                     /locus_tag="VHE33_00400"
     CDS             508..2295
                     /locus_tag="VHE33_00400"
                     /inference="COORDINATES: protein motif:HMM:NF016439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HVT95933.1"
                     /translation="MVLLQPKLSLKVAQRQILTPGLMQMVSVLALNKLELKEMIQAEI
                     AENPVLEEMDENVPLLDDVARAEEDRDRPVETQTVTEGETKEKEKDPFDEIDFGSYFQ
                     DYLDPGFRSPNNYEEIEKPSIENFLSAPATLTDHLFWQLGALNLSPAVREAAEYVIGN
                     LNEDGYLTATDDELLAGYLREQMEPDTAGDHAKNLYERVAELPPEMTERARGHLTTAL
                     DVVRQLDPIGVGTRDLRECLLVQVEAQKKEFNLIFARTQLGSKSNGHAAMSNGIDSSA
                     PEPTPAHPQAETGQTNGASGAGYPAPLATQAAASGNGTNPQPAAPDDVSRLEIFEIAR
                     QIIDAHMQLLQKRDPRELSKVLGKSVEETQRAIDFIRTLDPRPGQRYNRSEARLIEPD
                     VAFVKRGDEWVVAMNEEDLPTLRLNQGYRRLLTQGDQGKDVKDYVKERYRSALQLMRN
                     IEQRKNTILRTCESIVRRQTEFLDKGVDALKPMMIKEVAEEIGVHPSTVSRAVSSKYV
                     HTPQGVYELRFFFSEGVNGPEGAATPLMLLKRKVKKLIEDEDPRKPLTDDQIATMLQG
                     QGIDVTRRTVAKYREDLRIPSTHQRRIRS"
     gene            2335..3000
                     /gene="raiA"
                     /locus_tag="VHE33_00405"
     CDS             2335..3000
                     /gene="raiA"
                     /locus_tag="VHE33_00405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HVT95934.1"
                     /translation="MQAEYTGRQHTVTPSLRRLGDEGIERIAKLLPKAVGAHIVLTSE
                     KYRKTADVAVKMRAGKIVGFAESNNMEQALREALDRAETQAIRYRKRQNAIKRQPKDE
                     KLPHEVMPVRARRASRTRSENPEPELQNGNAVAAGANGKRPSKTVVPVTVHTFPATRP
                     ISEPHVVRSLDSVALRPMTIEEAVKEAEFRDRDVFVFRRSDGVLCVLHRMRDGKMELI
                     EAP"
BASE COUNT          704 a         1042 c          952 g          560 t
ORIGIN      
        1 cgggcgcggg cggaaaagct gatcgagcaa ctgaatctgg ggcacatccg caaaacgcgc
       61 ggctatgctc tctccggtgg cgagcgccgg cgggtggaga tcgcgcgctg cctgtgcatc
      121 cagccgactt ttatcctcct ggacgagccc ttttcgggaa tcgatcccat tgcggtactc
      181 gacctccagg aaatcatctt cgacctcaag gccagcggca tcggcgtgct cattacggac
      241 cataatgttc gcgagacact ctcggtgacc gaccgtgcct acatcatcaa tgaggggcga
      301 atcttctacc acgggacgcc cgaccagctc ggaaacgacc ccgaggtgcg gcgcgtctat
      361 ctgggagaag gcttcagcct gggttaaccc tcatggttcc gctaattacc tgaggaacca
      421 gtcacataca gtaacggcaa cagaattgct gcgcgcgccg ggtgcaggga tgtaaactcg
      481 gggcattggg ccctttcgca gaggattatg gttcttcttc agccaaaact cagtctcaag
      541 gtcgcacagc gtcagattct gacgccggga ctgatgcaga tggtcagcgt tctggcgctg
      601 aacaagctgg agctgaagga gatgatccag gcggagatcg ccgaaaaccc ggttttggaa
      661 gagatggacg agaacgttcc tctccttgac gatgtcgcca gggcggagga ggatcgcgac
      721 cgcccggtcg agacgcagac ggtcaccgaa ggcgagacaa aggaaaaaga aaaagatccc
      781 ttcgacgaga tcgacttcgg ttcctacttc caggattacc tcgatcccgg cttccgctcg
      841 ccgaacaatt acgaggagat cgagaagccc tcgatcgaaa acttcctctc tgccccggcg
      901 actctgaccg accacctctt ctggcagctg ggcgcgctca atctttcgcc cgctgtgcgc
      961 gaagccgccg agtacgtcat cggcaacctg aatgaggatg ggtatctcac ggcgaccgat
     1021 gacgagctgc tggcgggcta tctgcgcgaa cagatggaac cggatacagc cggcgaccac
     1081 gcgaagaatc tctacgagcg tgtcgcagag ctcccgcccg agatgaccga acgcgctcgc
     1141 ggtcacctga ccacggccct cgatgtcgta cgacagctgg atccgattgg tgtgggcacc
     1201 cgcgatctgc gcgaatgcct gctggtacag gtggaggcgc aaaagaagga attcaacctg
     1261 atctttgcgc gcacgcaact cggttcgaag agcaatggcc acgcagccat gtcgaacgga
     1321 attgattcct ccgctccgga gccaacgccg gctcatccgc aagcggaaac gggccaaacg
     1381 aatggcgcca gtggagcagg atacccggcg cctctggcca ctcaggccgc cgccagcggc
     1441 aacggcacga atccccaacc ggccgccccc gatgacgtca gccgtctgga aattttcgag
     1501 atcgccaggc agattatcga cgctcacatg cagctgctgc agaagcgcga cccacgcgag
     1561 ctatccaaag ttctcggcaa atccgtcgaa gagacccagc gcgccatcga cttcatccgc
     1621 actctggacc cccgccccgg ccagcgctac aaccggagtg aagctcggct catcgagccc
     1681 gatgttgcct tcgtcaagcg gggtgacgag tgggtagtcg ccatgaacga ggaagacctg
     1741 cctacgctgc gtctcaacca gggctatcgc cgcctgctca cgcagggcga ccagggcaaa
     1801 gacgtcaaag actacgtgaa ggagcgctat cgctccgcgc tgcagctgat gcgcaacatc
     1861 gagcagcgca agaacaccat cctgcggacg tgtgaatcga tcgtgcgccg ccaaaccgaa
     1921 tttctggaca agggcgttga cgccctgaaa cccatgatga tcaaggaagt ggctgaagag
     1981 attggagtgc acccgtccac tgtcagccgc gccgtatcca gcaaatatgt gcacactccg
     2041 cagggcgtct atgaactgcg tttcttcttc tccgaaggag tgaacggacc ggagggcgcc
     2101 gccacgccgc tgatgctgct caagcgcaag gtgaagaagc tgatcgagga cgaggatccg
     2161 cgcaagcccc tcaccgacga tcagatcgcc accatgctgc aggggcaggg catcgacgtc
     2221 acgcggcgca ccgtggcaaa ataccgcgaa gatctgcgca ttcccagcac ccatcagcgt
     2281 cgtatccgca gctagaggca gctgcggcat ctccccttaa gacaggagct ttccatgcag
     2341 gccgagtaca caggaaggca gcataccgtt accccttcgc ttcgccggct gggcgacgag
     2401 gggatcgagc gcattgcgaa actgttgccc aaagcggttg gcgcgcacat cgtgctgacc
     2461 tcagagaagt accgcaaaac cgccgacgtg gccgtgaaaa tgcgcgccgg caaaatcgtg
     2521 ggcttcgccg agtcgaacaa tatggagcag gcgctgcgtg aagcgctcga ccgtgccgaa
     2581 acccaggcca tccgctaccg caagcggcag aacgccatca agcgccagcc caaggatgag
     2641 aaacttcccc acgaagtgat gccggtgcgc gcgcgtcgcg cgagccgcac gcgctcggag
     2701 aatccggagc cggagttaca aaacggcaac gccgtcgctg ccggcgccaa cggcaagcgg
     2761 ccctcgaaga cggtcgtccc ggtgaccgtc cacaccttcc ccgccacacg ccccatctcc
     2821 gagcctcacg ttgtccgctc tctcgattcg gttgccctgc gtcccatgac catcgaagag
     2881 gccgtgaagg aagccgaatt ccgcgatcgc gacgtcttcg tctttcgccg ctccgatggg
     2941 gtgctgtgtg tcctgcaccg catgcgcgac ggcaaaatgg agctgatcga ggcgccctga
     3001 aagcagcgga tggcgtttgc ggttagcgtg tccggtagac cgacagtatt tccatccgcc
     3061 agacgctatc tgctcaacga cgatcttcac acatccagat ccagatccag ccctcgaatc
     3121 gcgtcgttca gcgcctgcgc cgccaccttg cgctgctgat ccagatccgc gcgctgcttc
     3181 ttgagcgcgt ccagctgatc ctcgtcgctg ttcatctcac ccgcatatcg gcgggccagc
     3241 gttttctcct cctgcgtg
//
LOCUS       DASZYN010000017         1830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_900242, whole genome shotgun sequence.
ACCESSION   DASZYN010000017 DASZYN010000000
VERSION     DASZYN010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900242"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VHE33_00410"
     CDS             <1..618
                     /locus_tag="VHE33_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95935.1"
                     /translation="TPDLRILISGPGTADFSLRVNNQGDTCFDNHGDHAPYVLATNLF
                     EGGAYRVQPNQRVLFEHGSLKQVVDNEQNSCGCPPPEPTPAPTAIARVGAPGITLHTS
                     NETNTPPPSPRSTETENPFPLAQSEGLKAPSTPPSTPAVPPGQTQTQITVPLTYNGEA
                     APSSGTPPPATQAAQPSASKAPSKPPEKPKKKHSAFVRFLRKIFG"
     gene            632..1042
                     /locus_tag="VHE33_00415"
     CDS             632..1042
                     /locus_tag="VHE33_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95936.1"
                     /translation="MRWGLLSRILAALLIPVVTLTTAAQFTPPVSKREAAVRRKAATL
                     TPQSPISVVRFGADEEFGRFLSCDQDGFTFYDIDQKRNVTLTWAEVKKIKDGYGGYNS
                     LRGRHTDRTKGLVVGLALAGALAGLIAAVATAKD"
     gene            1135..>1830
                     /locus_tag="VHE33_00420"
     CDS             1135..>1830
                     /locus_tag="VHE33_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95937.1"
                     /translation="MATASAVGRPGMWHTRATWSAREDRTIFLVWLALLWIGVLAGFG
                     VDIPRFLHEVPPPRLVVHVHAFVFTGWMFLLTLQILLVVGDRVALHRRLGWFTAGWAA
                     LMAVLGPWAAMVAKGPVPSGPPSPQFLSIQFGGIGAFLVLLAWGITLRKNPAAHKRIM
                     ILATVALIDAGFGRVAGWLFPEPKSMLAWYFWEFYGTLLILLLMTAWDAWRGRLMKQF
                     VIGAVGLFLLEWVQ"
BASE COUNT          340 a          687 c          482 g          321 t
ORIGIN      
        1 acgcccgatc tccgcattct catctccggt cccggcacgg ccgacttcag cctgcgcgtc
       61 aacaatcagg gcgatacctg cttcgacaac cacggcgacc acgcccccta cgtcctggcc
      121 accaacctct tcgaaggcgg cgcctaccgc gtccagccca accagcgcgt cctcttcgag
      181 cacggcagcc tgaaacaggt cgtcgacaac gaacagaact cctgcggatg cccgccccct
      241 gaacccaccc ccgcacccac ggcgatcgct cgcgtcggcg cgcccggcat cacgctccac
      301 accagcaacg aaaccaacac gcctccaccc agcccccgct ccaccgaaac cgaaaatcct
      361 ttcccgctcg cccagagtga aggactcaaa gcgccgtcca ccccgccatc gacgccggcc
      421 gttccccctg gccagaccca gacgcagatc accgtgccgc taacctacaa cggtgaagcc
      481 gctccgagta gcggaacgcc gccgccagcg acccaggcag cccagccgag cgcttcaaag
      541 gctccgtcta agccgccaga aaaacccaaa aagaaacact cggctttcgt ccgcttcctg
      601 cgtaaaatct tcggctaaga acggtagtcc aatgcgttgg ggccttcttt cacgaatcct
      661 cgcggcactc ctcattcccg tcgtcactct gacgaccgcc gcgcagttca ctccgcccgt
      721 atccaaacgt gaagccgccg tccggcgcaa agccgccaca ctcaccccgc agtcccccat
      781 cagcgtcgtg cgcttcggcg ccgacgagga gttcggccgc ttcctctcct gcgatcagga
      841 tggcttcacc ttctacgaca tcgaccagaa aaggaacgtg accctgacct gggccgaggt
      901 gaagaagatc aaagacggct atggcggata caactccctc cgcggccgtc acaccgaccg
      961 caccaaaggc ctggtcgtag gactcgccct cgcaggagcc ctcgccggcc tcattgccgc
     1021 cgtcgccacc gcaaaggatt agccccaccg acaaacagcc cgtctcccgc ttccccaact
     1081 tcctcacagg ctgatagcat cgctcaacat cccccggcag gagacagaaa agagatggcg
     1141 acagcatccg ctgttgggcg gcctggtatg tggcacacgc gcgccacctg gagcgcgcgc
     1201 gaagatcgga cgatattcct cgtatggctg gcattgctct ggatcggcgt cctcgccggt
     1261 ttcggcgtcg acataccccg atttctccat gaagtcccgc ctccgcgcct ggtggtccac
     1321 gtccatgcct tcgtcttcac cggctggatg ttcctgctca ccctccagat cctgctggtc
     1381 gtcggcgatc gcgtcgccct gcaccgaaga ctcggctggt tcactgccgg gtgggcagcc
     1441 ctgatggccg ttctcggtcc gtgggcggcc atggtcgcga aagggcccgt tccctccgga
     1501 ccgccgagtc cgcaattcct gtccatccag ttcggcggca tcggcgcgtt tctcgtcctg
     1561 ctggcgtggg gcatcaccct gcggaagaac cccgcggctc ataaaaggat catgatcctc
     1621 gccaccgtcg cgctgatcga tgcaggattt ggccgcgtgg ccggctggct tttcccggag
     1681 ccaaaatcga tgctggcctg gtacttctgg gagttttatg gaactctgct gatcctcctc
     1741 ctcatgacgg cctgggacgc atggcgcgga cgtctcatga agcagttcgt catcggcgcc
     1801 gtcgggctct tcctgcttga atgggtccag
//
LOCUS       DASZYN010000018        15939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1028772, whole genome shotgun sequence.
ACCESSION   DASZYN010000018 DASZYN010000000
VERSION     DASZYN010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 15939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15939
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028772"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VHE33_00425"
     CDS             <1..256
                     /locus_tag="VHE33_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HVT95938.1"
                     /translation="NGHRMPNAPPVPLPPSRAPSTTIDDIEKEFQRKKQKELEHARMA
                     GGGDSSGPSQRRVGDKVGRNDPCPCGSGKKYKKCHGATEG"
     gene            complement(381..656)
                     /locus_tag="VHE33_00430"
     CDS             complement(381..656)
                     /locus_tag="VHE33_00430"
                     /inference="COORDINATES: protein motif:HMM:NF039620.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HVT95939.1"
                     /translation="MHTLLFEGFLEGVVATTSLAAGLFFLRYWRDTRDSLFLAFSIAF
                     FIEGINRCVRIFFINPSEAAPWVYVVRAFAFLIILAGIVNKNRRSGA"
     gene            complement(656..928)
                     /locus_tag="VHE33_00435"
     CDS             complement(656..928)
                     /locus_tag="VHE33_00435"
                     /inference="COORDINATES: protein motif:HMM:NF039620.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HVT95940.1"
                     /translation="MNAIAPAVYILGFLVTLACGVLLFRAYSAVGKRLLLWSAVCFCG
                     LAISNLLVFFDLVVLPTQVDLYPWRLLTAAAAMLILLYGLIWEGQQ"
     gene            complement(931..1317)
                     /locus_tag="VHE33_00440"
     CDS             complement(931..1317)
                     /locus_tag="VHE33_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95941.1"
                     /translation="MPSATEENPRRVEVDRFILDEIDSVPHIEALLILWNSRPRTLSV
                     EELAGLLFLPQDHTRQILEDLQQRTLVVSGETGNAYNPAYPRDHLMADVDRTWRRELV
                     RVSNMIHSKASPAVREFARAFRFKKD"
     gene            complement(1429..1767)
                     /gene="cutA"
                     /locus_tag="VHE33_00445"
     CDS             complement(1429..1767)
                     /gene="cutA"
                     /locus_tag="VHE33_00445"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HVT95942.1"
                     /translation="MIVPATEVRIVITSAGSREEADDIARNLVDERLAACVSVVPGVS
                     SIYRWKGEVEAASENLLIIKTTAARLDGVEAALRRLHSYEVPEFLVLTPDGAGKSYLD
                     WLLFEAGNHS"
     gene            1838..3235
                     /locus_tag="VHE33_00450"
     CDS             1838..3235
                     /locus_tag="VHE33_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HVT95943.1"
                     /translation="MTGEIKFGTSGWRAIVAEDFTFGNIRRAVAGIAQYVGAQNGPKT
                     VLVGRDPRFLGEMFVAEAAKVLAMHSVTPLVIPDPAPTPAIAYGVRRLKTGGSINFTA
                     SHNPPEYNGIKYSTPDGAPALPEVTQQIEANVKKLDDVPSPTGKDTKFEQVDVKPDYL
                     KRIAELVDLKAIQKAGLKVVFDPFWGAARGYSCHVLREHGVETWTVHDFRDVLFGGHA
                     PEPDGELLDGCREKMKEVGAAIGIATDGDADRFGIVDADGTFIQPNYIIALLFDYLVE
                     TRGWKNGVAKSVATTNLINALADEFKVQLYETPVGFKYIGELINEDKIAIGGEESAGL
                     TIRGHVPEKDGVIAGLLAAEMVAVRGTSLGDQLKKLFARVGSFYPHRENFRLTPDVKE
                     KFTEKIKNDPQEIGGRRVGQVVRTDGLKLILADGSWVCYRLSGTEPVVRVYSEARKES
                     DQAPLQKAAKEWVLQ"
     gene            3310..3684
                     /locus_tag="VHE33_00455"
     CDS             3310..3684
                     /locus_tag="VHE33_00455"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HVT95944.1"
                     /translation="MANGEQKQLGRLERAAQVVYRVRRRIATAAVIAAALVFGYHAIF
                     GENGLNVYEQRRSEDRAVQKKIVELQRENARLSLHIKELKSDPDAIEREARERLHYAR
                     PGEVIWTEDQPLGTEADPSVRK"
     gene            3765..5072
                     /gene="murA"
                     /locus_tag="VHE33_00460"
     CDS             3765..5072
                     /gene="murA"
                     /locus_tag="VHE33_00460"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HVT95945.1"
                     /translation="MDKFVIRGGNPLIGTIRVSGSKNSALPCMAAALLTEDEVVLENI
                     PQVRDIETERKLLVSMGGEVELGYGRAQHRTTINCRTIKDPTAPYEIVKTMRASSLVL
                     GPLVARMGVARVSMPGGCAIGARPIDLHTKGLEMLGAKISYEHGYIEARAERLKGAHI
                     VFDKITVTGTEDLLMAAALADGETVMENCAREPEVTDLAAMLTAMGAQIHGAGSSTIH
                     VKGVTRLRGVRHRINPDRIEAGTFLVAGAITGGDLCVAHCNPSHLTALIGKLQECGVR
                     VEIIAKDQIRVRSEGALKAADISTEEYPGFPTDMQAQYMALATQAEGTSLVKENIFEN
                     RFMHVQELVRMGANIRVDGRTATVRGRSPLSAAAVMCSDLRASASLVLAAMVADGESI
                     LDRVYNIDRGYEHIEEKLRGVGAQIRRMGNVFQEKREPEMAPA"
     gene            5161..5646
                     /locus_tag="VHE33_00465"
     CDS             5161..5646
                     /locus_tag="VHE33_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HVT95946.1"
                     /translation="MAKTEDRALRELLVAFLSGREAHAEFKNVLDDFPVAARGAVPKG
                     AEHSAWQELEHIRIALHDLLDFSTNPNYVGMKWPDDYWPKEAAPKNDDAWDASVRAVK
                     KDMAAFEKLVGDPESNLYATFPWGEGQTLLREVLLAGQHTSYHLGQIVLLRRMLGAWK
                     G"
     gene            complement(5643..6656)
                     /locus_tag="VHE33_00470"
     CDS             complement(5643..6656)
                     /locus_tag="VHE33_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95947.1"
                     /translation="MIETKIFDPADGFAPITDLAEITDPTVVKRGNQWWMFAAGQIHG
                     DHAQHLFSASLPGGAPLSARGWQLTARSDDPSKIAALPRVRSRAWDGAGGRHCPSFVR
                     AFDPHTGQGIERLYYANSAANPWGPYTIAFLEWDGEQWIDQPEPAFAATEAWEHGSVY
                     EPNLIYADGQWRMWYVAGSNIDNYIVHGYAESADGRTGWTPHRVFAAPDEKMFDFCVA
                     KNSTGYEAVFSRVWLGQEAPPPQTGLWWCRADAPHPTLSSWSEPVQILTAEDKGWRTG
                     PWKPSVAFEESNPDSLFVFFDGIYRTSDPGPFPFAFTLGCVELDREELVRSCARSAVR
                     LDG"
     gene            complement(6747..8099)
                     /locus_tag="VHE33_00475"
     CDS             complement(6747..8099)
                     /locus_tag="VHE33_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HVT95948.1"
                     /translation="MLDSRIILITLLIKLGVAAAVSSALGRSREFQNLLFREDRNLGQ
                     TLGLLAFICIPLGLGVWVRVTVPNFYAADISFETTIILGILIGPGAALIGAALLSLPA
                     VLHREYLSLPFLLAVAVIAGIYGRFVDKEEVWSFSPFVDLSLYRWVRRTLLRPRIDRQ
                     ILLLILIVAMEILREWTERISHGRLFALVSDSWLIRLAVWACAAMVVGIPLKIWNAIR
                     IEQSLEEQKRLLLEARLDALQRQINPHFLFNTLNSIASLVRFRPEQARELIVKLANIL
                     RKMLNEHDAFVPFRDELGTTDDYLSIEIVRFGAEKLSVEREIDPQTLEIPVPSMLLQP
                     LVENSIKHGLEPRIAGGVITLRSRIEQGRLIIEVEDDGVGIAPGRAHTSGVLQGTGNG
                     IGMRNVRERLEVLYGDAAMFDVTSRPGRGTKVTLAFPAQTANHEHPATRAALATDQGR
                     "
     gene            8202..8852
                     /gene="ribA"
                     /locus_tag="VHE33_00480"
     CDS             8202..8852
                     /gene="ribA"
                     /locus_tag="VHE33_00480"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HVT95949.1"
                     /translation="MAIASVRKVADADFPTRWGHFRILGFEGVYAADDPARCGQTAEK
                     EEAVALVMGDIQSTAPLVRIHSQCLTGDVFHSLRCDCRLQLELALRRIVDAGAGILIY
                     EQQEGRGIGLLAKLRAYELQDQGLDTVEANLKLGFEADCREFELPAAMLRHMGVKAVR
                     LITNNPEKVAALESAGVHVAERVSAEVPAEDSFEPYLRVKQEKMGHIFESMDETTR"
     gene            complement(9478..9645)
                     /locus_tag="VHE33_00485"
     CDS             complement(9478..9645)
                     /locus_tag="VHE33_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95950.1"
                     /translation="MICARQWFNKLLYKQHSDTVEYVLLLLLLVFGAVATGQALTHQI
                     GNEFNKITSQF"
     gene            complement(9942..11132)
                     /locus_tag="VHE33_00490"
     CDS             complement(9942..11132)
                     /locus_tag="VHE33_00490"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HVT95951.1"
                     /translation="MKLRACFLFVAVTAFTFALPPAHALDNGVARTPPMGWNSWNKFA
                     CKGLNEQVVRATADAIASNGMKDAGYAFINLDDCWQSGRDAEGNIVVDQEKFPSGIKA
                     LADYVHSKGLKFGVYTDVGTKTCAGRPGSVGHEYQDARQYAAWGVDYVKEDWCNTLPG
                     QNAEASYTLMRDALASTGRPIVFSICEWGSNKPWLWARPIGNSWRSTGDIQDCWDCKK
                     PWGGNGLVQIIEQMADITYYSGPGHWNDPDMLEVGNGGMTTEEYRTHFSMWAMFAAPL
                     LVGTDVANMSADTKSILLNRAVIAVDQDALGEAARRVKKDGDIEVWSRPLADGSRAVT
                     LLNLSASPISTGIQWTEIGYPADLRATVRDLWTGKEVNDQTGHYASQVPSHGVVMVTI
                     KPHN"
     gene            complement(11259..11585)
                     /locus_tag="VHE33_00495"
     CDS             complement(11259..11585)
                     /locus_tag="VHE33_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95952.1"
                     /translation="MRMMLHVVIPVETGNAAIRGGQLGPFIQKVLGDLKAEAAYFTES
                     EGSRSGYIFFDMKDSSELPAIAEPWFLAFNATLTVKPAMTPQDLAAGGAGMEAAVKTS
                     PKTFGS"
     gene            11675..12514
                     /locus_tag="VHE33_00500"
     CDS             11675..12514
                     /locus_tag="VHE33_00500"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HVT95953.1"
                     /translation="MIQNRAFSILLAAALPLAAQTNHPAAAAHRATTATGHREASGCV
                     TLPELSPKIPALPAGSPCAKTLYTVTRTPTLKLDYASPLVSQGVREELGDKPETFSLD
                     YVDTKIGTGNPIEPHKCISVQYTGYLANGTKFDSSRDRNQPIEFLYGGHRVIPGWDTG
                     FEGMRMGGERRLFIPWQLAYGENGRGPIPPKAELIFDVEAVSQSEPRAAGGPPGSECM
                     KTPPPQPHRPEVPPAGDAPKGATTTPPTGTTPPAEKSEPATPPPATSTPPASTQSQQT
                     QPH"
     gene            complement(12561..13451)
                     /locus_tag="VHE33_00505"
     CDS             complement(12561..13451)
                     /locus_tag="VHE33_00505"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT95954.1"
                     /translation="MPEADRIMRLAFGTFLGLPDPMAFMGDRNFMAPRFRAKHVKVLG
                     ARLDARLIGVVAATRWGAFGFFGPLVVLPEFWDKGVAQRLLEPTTKLFDRWGVRYSGL
                     FTFAHSAKHVGLYQKFGYWPTYLTALMTYTPDAAPQTALPTVLSNLPRQQREQAIVAC
                     AQLTNRIDKGLDLTEEIRTVLTQRTGDVVLIFTRATLEGFAICVHGAGSEAGTRTCYV
                     KFGAARNDNSFDRLLAACEAFAGSHGVNLEAGVNFARENAYRRMKARGYRTMTQGVAM
                     LRPHVAGFNRTNAYVIDDWR"
     gene            complement(13632..14177)
                     /gene="lepB"
                     /locus_tag="VHE33_00510"
     CDS             complement(13632..14177)
                     /gene="lepB"
                     /locus_tag="VHE33_00510"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HVT95955.1"
                     /translation="MFQPRLRAWARRLFQFRVWARDIGISVIASFFIVTVLYQPVRVE
                     GTSMQPELRDQDRLFIDKLFFHFEKITRGDVVVFHYPRDPEKSYIKRVVALPGDSVFI
                     DHGRVWINGAPLDEPYVPRRYRDTRSMPEMIVPTDEYFVMGDHRSISSDSRDFGPVDR
                     ELIYGKATFIYWPADEMGVVR"
     gene            complement(14333..15205)
                     /locus_tag="VHE33_00515"
     CDS             complement(14333..15205)
                     /locus_tag="VHE33_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95956.1"
                     /translation="MTRVRFTLAAIVLTVSLTAAAQDATPASAPMPSTAAPTATQTAE
                     ATPPAVAAAAPPATILVPAGTRVLLELRSSVNTKSAKPGDGVYLSSTFPVIVGSRVVI
                     PAGVYVQGVVDNVVRPGRGIGAKKAQLNMHFTSMIFPNGSVVEIPGVVNSLPGADHQK
                     VNGDEGTIQQDGGSNKAHNAGETAKVAVPTGATIGSIGGLEGGHPLAGGLEGAGAGLA
                     AAGIVALFTRGADVNIASGTQVEMVLQRPLQLQEANLNGPTMLVPASTQPQPLPKPGR
                     PAHILCPTGSLGCS"
     gene            complement(15404..15829)
                     /locus_tag="VHE33_00520"
     CDS             complement(15404..15829)
                     /locus_tag="VHE33_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95957.1"
                     /translation="MRPVLALRIASVLTFIHAVLHTVGGVFGAIPPGPASIAVEAMKS
                     NQFVALGVTRTFWQYYHGMGLAVSIFLFIEAIVFWQLSSLAKTIGPQLRPVFASFMAA
                     YLAIAVNSYLYFFLAPVIFEIVIALCFLWAILSAKPAST"
BASE COUNT         3382 a         4310 c         5310 g         2937 t
ORIGIN      
        1 taacggacat cgcatgccga acgcgcctcc tgttccgctg ccgccctctc gagcgccttc
       61 gacgaccatc gacgacatcg agaaggagtt ccagcggaag aaacagaagg aactggaaca
      121 cgcacggatg gcaggcggag gagattcgtc tggaccgtcg cagcgacgcg tgggagataa
      181 ggtcggccgg aatgacccct gcccctgtgg atcaggcaaa aagtacaaga aatgccatgg
      241 ggcgactgaa ggctagagcg gccaattggc gatcaggaac agccaatagc gagtgaggtg
      301 cggggatgat tcttcgcacc ttttctgttt cagaccgccg caatgaacgg aggctgcggg
      361 acgcaggaaa gcgcgcgatc ttaggcgccg gagcggcggt tcttgttcac aatgccggcc
      421 agaatgatga ggaaggcgaa ggcgcgcacg acgtagaccc agggtgcggc ctcactggga
      481 ttgatgaaga agatgcggac gcagcgattg atcccctcaa tgaagaaggc gatcgagaag
      541 gcaagaaaga gggagtcgcg cgtgtcgcgc cagtagcgga gaaagaagag gcctgccgca
      601 agcgatgtcg ttgctactac accttcaagg aagccttcaa acaggagagt gtgcattatt
      661 gctggccctc ccagatgagt ccgtacagga gaatcagcat ggcggcggcg gcggtgagga
      721 ggcgccaggg ataaagatcg acctgcgtcg gcagaaccac aaggtcaaag aacacgagca
      781 gattggagat cgcgaggccg cagaagcaaa cagcgctcca caacagcagg cgcttgccga
      841 ctgcggaata ggcgcggaag agaagcaccc cgcaggcaag ggtcaccagg aacccgagga
      901 tgtagacggc aggggcgatg gcgttcatgg tcagtctttc ttgaacctga aggcgcgggc
      961 gaattcgcgc accgcgggcg aggctttgga gtggatcatg ttggaaacgc gcaccaactc
     1021 acggcgccag gttcgatcga catcggccat gaggtggtcg cgagggtaag cggggttgta
     1081 ggcgttgccg gtttcgccgg aaacaacgag cgtgcgctgc tggagatcct cgaggatttg
     1141 ccgggtgtgg tcctggggga gaaagagcag gccagccagt tcttcgacag acaacgtcct
     1201 cggccgactg ttccagagga tcaacagggc ctcgatatga gggacactat cgatctcgtc
     1261 gagaatgaag cggtcgactt cgacgcggcg cggattctct tccgttgccg agggcatgac
     1321 ttgcgttcag agtgtctaag gaaccaggct aaacggtttt cgatgcgaaa agcgcagatc
     1381 agcgttgcac atgaaaatta attgcctggc atgggcacag gtatcgcatc acgagtgatt
     1441 tccggcttca aaaagaagcc agtcaagata cgatttgccc gcgccgtccg gagtaaggac
     1501 gagaaattca ggaacttcat acgaatgcag ccggcgcagg gcggcttcga caccatcgag
     1561 cctggcggca gtagtcttga tgatgagcag gttttcggag gcggcttcga cctcgccttt
     1621 ccagcgatag atcgagctaa cgcccgggac gacgctgacg caggcggcga ggcgttcgtc
     1681 cacgaggttg cgcgcaatgt cgtcggcctc ttctcgagag cctgccgacg tgatcacgat
     1741 acggacttcg gtggcgggta caatcatcgg tggcttgcgg ttacggcagg aattccggct
     1801 ggaagcatca taaggccgag tggattcgag gggacggatg acgggcgaaa tcaaatttgg
     1861 aacgtccggg tggcgggcga ttgtcgcaga ggattttaca tttggaaata tccggcgagc
     1921 ggtggcgggc atcgcgcagt acgtgggcgc gcagaatgga ccgaagaccg tgctggtggg
     1981 ccgcgaccca cgatttctgg gggagatgtt tgtggcggaa gcggcgaagg tgctggctat
     2041 gcacagcgtg acgccgctgg tgatccctga ccccgcgcct acaccggcga ttgcctatgg
     2101 agtgcgtcgg ctgaaaacgg gagggtcgat caacttcacc gcttcgcata atccgccgga
     2161 gtacaacggc atcaagtact cgacgccgga tggggcgccg gcgctgccag aggtgacgca
     2221 gcagatcgag gccaacgtaa agaagctgga cgacgtgccg tcgccgacag gcaaggacac
     2281 aaagttcgag caggtggacg ttaagccgga ttacctgaag cgcattgccg agctggtgga
     2341 cctgaaggcg atccaaaaag ccgggttgaa ggtggtgttc gatccattct ggggcgcggc
     2401 ccgcggctac agttgccatg ttctgcgcga acatggcgta gaaacctgga cggttcatga
     2461 ttttcgcgat gtgctctttg gcgggcacgc gccggagccg gacggcgaac tgctggatgg
     2521 ctgtcgagag aagatgaagg aggttggagc ggcgatcggg atcgcaacgg acggcgatgc
     2581 ggaccgcttt ggcattgtag atgccgacgg cacctttatt cagccgaact acatcatcgc
     2641 gctgctgttt gattatctcg tcgagacccg cggctggaaa aacggcgtcg cgaaatcggt
     2701 ggctacaacc aatctgatca atgccctggc cgatgagttc aaggtgcagc tctacgagac
     2761 gccggtggga ttcaagtaca tcggcgagct gattaacgag gacaagatcg cgattggcgg
     2821 tgaagagagc gccgggctca cgattcgtgg ccatgttccg gaaaaggatg gcgtgattgc
     2881 cggccttctg gctgctgaga tggtggcggt gcgcggaacc agcctcggcg accagctcaa
     2941 gaagctattt gccagggttg gttcctttta tccccaccgg gagaattttc gcttgacgcc
     3001 tgacgtgaag gaaaagttca ctgagaagat aaagaatgat ccgcaggaga tcggcggccg
     3061 aagggttggg caggtggtgc gcaccgatgg cctgaaactg atcctggcgg atgggtcatg
     3121 ggtttgctat cggctcagtg gaaccgagcc ggtggtcagg gtctattcgg aggcgcgaaa
     3181 agagagcgat caggctcctc tgcagaaagc cgcgaaagaa tgggtactgc agtgatcagt
     3241 ctcagatttc gaaccggttc atgagcgaga cggagcgcaa ggcggttggc ggtgggcggg
     3301 agttgagcag tggctaacgg agaacagaag caactcggac ggctggagcg cgccgcgcag
     3361 gttgtgtatc gcgtgcggcg gcgtatcgct acggctgcgg tgatcgcggc ggccctggtc
     3421 tttggatacc atgcgatttt cggcgaaaac ggactgaacg tctacgaaca gcgccggagc
     3481 gaggaccgcg cggtgcagaa aaagattgtg gaacttcaaa gggagaacgc ccggctgtcg
     3541 ctgcatatca aggagctgaa gtcggatccc gatgccatcg agcgcgaggc acgagagcgg
     3601 ctgcactacg cacgtcccgg agaagtgatc tggactgaag accagccgct tggtaccgag
     3661 gccgatccat cggtccggaa atagctcctg aagcagacag cgccgtcgct cgctggttgc
     3721 tgctgcatct tttcctgaag acacgcattt aatctgaaga ggctatggat aaattcgtca
     3781 ttcgcggcgg aaatcccctt atcggcacga ttcgggtgag cgggtcgaag aattcagcgc
     3841 tgccctgcat ggcggcggcg ctgctgactg aagatgaggt ggtgctggag aacattccac
     3901 aggttcgcga tatagagacc gagcgcaagc tgctcgtttc gatgggcggc gaagtggaac
     3961 tgggctacgg acgcgcgcag catcgcacaa cgattaattg ccggacgatc aaggatccca
     4021 ccgctcctta cgagattgtc aagaccatgc gggcgtcgtc gctggtgctg ggtcctctgg
     4081 tggcgcggat gggggtcgcg cgggtgtcga tgcctggtgg ctgtgcgatc ggagcgcgcc
     4141 ccatcgacct gcacaccaaa ggcctggaga tgctgggcgc gaagatcagc tacgagcatg
     4201 gctacatcga ggcgcgtgcg gagcggctga agggggcgca tatcgtattc gacaaaatca
     4261 cggtgacggg taccgaggat ctgctgatgg cggccgccct ggccgacggt gaaaccgtga
     4321 tggaaaactg tgcgcgcgag cccgaggtga ccgatctggc ggcgatgtta acggcaatgg
     4381 gtgctcagat tcatggcgcc ggaagctcaa ccatccatgt gaagggcgtg accagactgc
     4441 gcggggtaag gcatcgcatt aatccggatc ggatcgaggc tgggacgttt ctggtggcgg
     4501 gggcgatcac gggtggcgac ctttgcgtcg cccactgcaa cccgtcgcat ctgacagccc
     4561 tgatcgggaa gctgcaggag tgcggcgtgc gcgtcgagat catcgcaaag gatcagattc
     4621 gggttcgcag cgaaggcgcg ctcaaagcag cggatatctc gacggaagag tatccaggat
     4681 ttccgaccga tatgcaggcg cagtacatgg ctctggcgac gcaggcggaa gggacctcac
     4741 tggtgaagga gaacatcttc gagaaccgct tcatgcacgt acaggaactg gtacgtatgg
     4801 gggcgaatat tcgcgtggat ggccgcaccg caaccgtccg cggcaggagc ccactttctg
     4861 ccgctgcggt gatgtgctcg gatttgcgcg cgtcggcttc tctcgtgctg gcggctatgg
     4921 tggccgacgg cgagtcgatc ctggatcgcg tgtacaacat cgatcgcggc tacgagcata
     4981 tcgaggaaaa actgcgcggc gtgggggcgc agatccggcg tatgggcaac gtcttccagg
     5041 aaaagcgcga gcccgagatg gcgccggcct gaaagcaggg tgcagggtac gcgaaggttc
     5101 ggcgaaatgc cgaacctttg ctatttggtt gcgcatctga tcgcgaagag gagaagtgcg
     5161 atggcgaaga cggaggaccg tgcactgcgc gaactgctgg tggcttttct gagtgggcgc
     5221 gaggcgcatg cggagttcaa gaatgtgctg gacgattttc cggtggccgc tcgcggggcg
     5281 gtgccgaagg gtgcggagca ctcagcatgg caggaactgg agcacattcg gattgcactg
     5341 cacgacctgc tggacttttc caccaacccg aactacgtcg gtatgaagtg gccggacgac
     5401 tattggccga aggaagcggc gccgaagaac gacgatgcat gggatgcatc ggtgcgcgcc
     5461 gtgaagaagg acatggcagc attcgagaaa ctggtcggcg atccggagtc gaacctttac
     5521 gcgacctttc cctggggtga ggggcagacg ctgctgcgcg aagtactgct cgcggggcag
     5581 cacaccagct atcacctggg gcagatcgtg ctgttaaggc gaatgctggg ggcctggaag
     5641 ggctagccat ccagccgcac cgcgcttcgc gcgcatgatc gtacgagttc ttcccgatcc
     5701 agttctacgc agcccagcgt gaaggcaaac gggaaggggc ccggatcgct ggtgcggtag
     5761 atgccgtcga agaagacaaa gagggagtcg gggttcgact cctcaaaggc gaccgagggc
     5821 ttccaggggc cggtacgcca gcccttgtct tcggcggtga ggatctggac cggctcgctc
     5881 caggaggaga gggttggatg gggggcgtcg gcgcggcacc accatagtcc ggtctgcggc
     5941 ggtggggctt cctggccgag ccagacgcgg gagaaaaccg cctcgtagcc ggtggagttt
     6001 tttgcgacgc agaagtcgaa catcttttca tccggcgcgg cgaagacgcg gtgcggggtc
     6061 cagcctgttc ggccgtcggc gctttccgcg tagccatgga cgatgtaatt gtcgatgttc
     6121 gagccggcta cgtaccacat tctccattga ccgtccgcgt agataagatt gggctcgtag
     6181 acgctgccat gctcccacgc ttcggtcgcg gcgaacgcag gttcgggctg atcgatccac
     6241 tgttcgccgt cccactcgag aaacgcgatg gtgtagggtc cccatggatt ggcggcggag
     6301 ttggcgtagt agaggcgttc gatcccctga ccggtatggg gatcgaaggc gcggacgaac
     6361 gaagggcagt ggcgaccgcc cgctccgtcc caggctctgc tgcgaacgcg tgggagtgcg
     6421 gcaatctttg agggatcgtc ggagcgcgca gtgagctgcc agcctcgtgc ggagagcggg
     6481 gcgccgcctg gcagggatgc gctgaacagg tgctgggcgt ggtcgccgtg gatctggccg
     6541 gcggcgaaca tccaccactg attaccgcgt ttgacgacgg tggggtcggt gatctcggcg
     6601 agatccgtga taggggcaaa gccgtcggcg gggtcgaaga ttttggtttc gatcatgggg
     6661 aaaagcaggg atcaggattt acgggatagg cgcagcggat tcgacaatac agcttgcgac
     6721 cctatcgctc gtggagaagg agaattctac cttccctggt cggtagccag ggccgctcgg
     6781 gtggcgggat gctcatggtt ggcggtctga gcgggaaagg cgagcgtcac tttggttcca
     6841 cggcctgggc ggctggtcac gtcaaacata gctgcatcgc catagagaac ttccagtcgc
     6901 tcgcggacgt tgcgcatccc gatgccgttg cccgtgcctt ggaggacgcc gctggtgtga
     6961 gcacggccag gcgcgatgcc gactccatcg tcttcgactt caatgatgag cctgccctgt
     7021 tcgatgcggc tgcgcagggt gatgacaccc ccggcgatgc gtggctcaag cccgtgcttg
     7081 atgctgtttt cgaccagagg ctgcagcagc atactgggca caggtatttc cagagtctgt
     7141 ggatcgattt cgcgctctac ggagagcttc tccgcgccaa agcggacgat ctcaatggag
     7201 agatagtcgt cggtagttcc cagctcgtcg cggaagggca caaaggcgtc gtgctcattg
     7261 agcattttgc gaaggatgtt ggcgagtttg acgatgagtt cgcgggcctg ttccgggcgg
     7321 aagcggacca gcgacgcaat ggaattgagc gtgttgaaga ggaagtgcgg gttgatctga
     7381 cgctgcagcg cgtcgagtcg ggcttcgagg aggagacgct tttgctcttc gagggactgc
     7441 tcgatgcgga tagcgttcca gattttgagc ggaataccga cgaccatggc agcgcaggcc
     7501 cagacggcga ggcgaatgag ccagctgtcg gagacgaggg cgaagaggcg cccgtgcgaa
     7561 atgcgctccg tccactcgcg cagtatctcc attgcgacga tgaggatgag gagcaggatc
     7621 tggcggtcga tgcgtgggcg gagcagcgtc cggcgaaccc agcggtagag gctgaggtcg
     7681 acgaatggag aaaaggacca gacttcctct ttgtctacaa agcggccgta aatgccggca
     7741 atgacggcca ccgcaagaag gaagggaagg gagagatatt cgcggtgcag gacggccgga
     7801 agcgagagaa gagcggcacc gatgagggcg gcgccaggcc cgatcagaat gcccaaaatg
     7861 atggtggtct cgaaagagat atctgccgca tagaagttgg gcacggtaac gcgaacccag
     7921 acaccgagac cgagcgggat gcagataaag gcgaggagtc caagggtttg gccgaggttg
     7981 cgatcttcgc ggaagagaag gttctggaac tcgcgcgaac gtccgagggc cgaggagacg
     8041 gcggcggcca cccccagctt gatcagcagg gtgatgagga tgatgcgcga gtcgagcacg
     8101 tgtttagcct acagcggaca gcggataacg ttgaggaaaa tggcgttcgg aacgcgacat
     8161 ttcgaaaagt ggcgggcgaa ggccacttct ataatcaatc catggcgatt gcttcagtac
     8221 gcaaggttgc ggatgcggat tttcccacgc gatgggggca ttttcgcatt ctgggctttg
     8281 agggcgtcta cgcggccgac gatccggcgc gatgcgggca gaccgccgag aaagaagagg
     8341 ctgtggcgct ggtgatggga gacatccagt cgaccgcgcc gctggtgcgc attcattcgc
     8401 agtgtctgac cggcgacgtc tttcactcgt tgcgatgcga ctgccggttg caactggaac
     8461 tggcgctgcg aaggattgtc gacgccggtg ccggaattct gatctacgag cagcaggaag
     8521 gtcgcggcat cggcctgctc gctaagctgc gtgcctacga gctgcaggat cagggtctgg
     8581 atacggtaga ggcgaatctg aagctgggct tcgaggcgga ctgccgggag tttgaactcc
     8641 ctgcagcaat gctgcgccac atgggcgtca aagccgtgcg gctgattacg aacaaccccg
     8701 aaaaagtggc cgcgctggaa tcagccgggg ttcacgtggc cgagcgcgtt tcggcggaag
     8761 tgcccgcgga ggatagcttc gagccgtacc tgcgtgtaaa gcaggaaaaa atgggacaca
     8821 tctttgagtc gatggacgag acgacccgtt agctaatccg gaatggtgag cgacgcgtgt
     8881 ccacatacct ggatctcctt gcatgcaggc cggagtgttc cggcgcgtcg atttttagtt
     8941 gcctttgacg gcggcggcaa ggggaagagc aagacccatg cccgcgacaa taagcgacgt
     9001 acccgcgagt gcaatgatga gcaacatggc agaaaccctc cctggggaaa tgatgaagtt
     9061 tgtattcatc tccgcacttc gcaggtgccg gagataaagc tcttccgccg gtggagtctt
     9121 cgcttgcggt tggcaggaat gaagactcca cacagcggaa tcaagtgctg gagaacggtg
     9181 gcagaaacgg caaatgcgtc aggcagagca acaaggagaa acgcgatcag atggtttgat
     9241 tctgcccgcc ggggacaatc caaacaattc catttgcgta atggagcggc gatcaaatgg
     9301 aaacgggtct cttattactg cgctggtcgc ggcataaccc atccgtaagt tactcgcgta
     9361 ggaacgggta gggaagagag cgcgattccg attagagagg gggatcggca ggaggacggt
     9421 gacagctcgc gcagcggggt tggcacaccg agccacatca gggaatgaca ggcgagctta
     9481 gaactggctg gtgattttgt tgaattcgtt tccgatctga tgggtcagcg cctgcccggt
     9541 cgcgacggct ccaaaaacaa gaagcagcag caggaggaca tactcgaccg tatccgaatg
     9601 ctgcttgtac aggagtttat tgaaccactg tcttgcacag atcattggaa tttgtcctcg
     9661 gctgaatgcg ctggagaatc tggggactct acgggcatca cgctgaatag ggtgattctg
     9721 ccggtccagg ctgatcgacg ccattccctt tgcgtaatga aacagcgaac atttggaaac
     9781 ggggagtggt tactgtgtaa cgagagagaa ttccgggtcc gcgaggctgg ggagcggctg
     9841 cgtcgattgt gctttgcgaa ctggggccaa aggtctggct gggtccaact gctgcagggc
     9901 aacgagttaa gcgggtgccg cgcgatgacc ccagtgtcgg gtcagttgtg aggcttgatc
     9961 gtgaccatga cgaccccatg cgagggcacc tggctcgcat agtggccggt ctgatcgttg
    10021 acctctttgc ccgtccaaag atcacgtacg gtggcgcgca ggtcggctgg atagccaatt
    10081 tcggtccact ggatgcctgt tgatatcgga ctcgcactca gattgagaag ggtgacggcg
    10141 cggctcccgt cggcgagcgg ccgagaccaa acctcgatgt cgccgtcctt cttgacgcgg
    10201 cgggctgctt cgccgagagc gtcctggtcg acagcgatga cagcgcgatt gaggaggatc
    10261 gatttcgtgt ccgccgacat gttcgcgacg tcggtcccca ccaacagcgg ggcagcgaac
    10321 atggcccaca tgctgaagtg agtgcggtat tcctcggtgg tcatgccgcc gttaccgact
    10381 tcaagcatgt cagggtcgtt ccagtggccg ggtccggagt agtaggtaat gtcggccatc
    10441 tgttcgatga tctgcaccag cccattgcca ccccagggct tcttacagtc ccagcaatcc
    10501 tgaatgtcgc cggtcgaccg ccaggagttt cctatgggcc gggcccacag ccacggtttg
    10561 ttactgcccc actcacaaat gctgaagacg attgggcgcc cggtggaggc cagggcatcg
    10621 cgcatcaggg tgtaagaagc ttcagcgttc tgacccggca gggtattgca ccagtcttct
    10681 ttgacatagt ccacgcccca ggcggcatac tgacgcgcgt cctggtattc gtggccgacg
    10741 ctgcctggcc ggcccgcgca ggttttcgtt ccaacatcgg tatagacgcc gaacttcagc
    10801 cccttgctgt ggacgtagtc tgccagcgcc ttgatgccgg aggggaattt ctcctggtcg
    10861 acgacgatgt tgccttcagc atctcgcccg ctctgccagc agtcgtcgag gttaatgaag
    10921 gcatagccgg catccttcat gccgttgctg gcgatggcgt ccgctgtggc gcgcactacc
    10981 tgttcattca ggcctttgca ggcgaacttg ttccagctgt tccagcccat cggcggagtg
    11041 cgggcgacgc cattgtcaag tgcgtgggcc ggaggcaggg caaaggtgaa agcggttaca
    11101 gcaacgaaca gaaaacaggc gcgcagtttc atatgtggtg gctctccgag gtagtcgggg
    11161 gaattgtaca tccgtgccgg cgccggatga gagcggaatg aggagaactg ggggaggagg
    11221 agtgctgcag gagagcggaa cgacagcatc gcgcttcgct aagaaccgaa ggtcttcggc
    11281 gaagtcttga ccgcggcttc catgccagcg ccacccgctg caaggtcctg gggtgtcatc
    11341 gccggcttga ccgtgagagt agcgttgaaa gcaaggaacc agggctctgc gattgccggc
    11401 agctcggacg aatccttcat atcgaaaaaa atataaccgc tgcggctgcc ctcgctttca
    11461 gtaaagtaag cggcctcggc cttgagatcg cccaaaacct tctggatgaa cggacccaat
    11521 tgcccgccgc gaatcgcggc attgcccgtt tcgactggga ttacaacatg aagcatcatg
    11581 cgcatcgtgc agcccctcca tggggcgtgg cgggcattat acggacgtgg aggagagggc
    11641 gcacagaccc tggcatcgca tacactgggt agcgatgatc cagaatcgtg ccttctccat
    11701 cctcctggcc gccgccctgc cgctggccgc tcagacgaac catccggccg cggctgccca
    11761 tcgggccact accgccaccg ggcatcgcga ggcatccggc tgcgtgacgc tgccggaact
    11821 gtcgccgaag atccccgctc ttcccgcagg ttctccctgc gcgaagacgc tctacaccgt
    11881 gactcgtact ccgacgctga agctcgacta cgcatcaccc ctggtgagcc agggagtacg
    11941 tgaggaactg ggagacaagc cggagacctt ctcgctggac tatgtcgaca cgaaaatcgg
    12001 gaccggcaat ccgatcgagc cacacaaatg catcagcgtg caatacaccg gatacctcgc
    12061 gaatgggaca aaattcgact cctcgcgcga ccgcaatcag ccgatcgaat ttctgtatgg
    12121 cggccatcgc gtgattccag gctgggacac ggggttcgaa gggatgcgca tgggcggcga
    12181 acgccgtctg ttcatcccct ggcagctggc ttatggcgag aacggcagag ggccaatccc
    12241 accgaaagcc gagctgattt tcgacgtgga agcggtgagc cagagcgagc cgcgagccgc
    12301 gggcggacct ccggggagtg aatgcatgaa gactcctcct ccgcaaccgc accgccctga
    12361 agttcctccg gcgggggacg cgccaaaggg cgcaacgacg acgcctccaa ccgggactac
    12421 gccaccggca gagaagtccg agccggcgac gccaccgccc gcaacgagca ctccaccggc
    12481 ttcaacccag tcgcagcaga cccaaccgca ttagcgcagt tgggcagagt ccagtccgca
    12541 attggcgctg acccctcgga tcagcgccag tcgtcgatca cgtaggcatt cgtgcgattg
    12601 aatccggcca cgtgaggccg cagcatcgcg actccctgcg tcatggtgcg atagccgcgg
    12661 gctttcatgc gccgataggc attctcgcga gcgaagttta cgccggcttc gaggttgact
    12721 ccatgtgaac cggcaaacgc ttcgcacgcc gccaggagcc ggtcgaagga gttgtcattg
    12781 cgagcagcac cgaatttgac gtaacacgtc ctggtgcctg cttcagagcc ggcgccgtga
    12841 acgcagatgg cgaagccctc cagcgtcgcg cgcgtgaaga tgagaacgac gtcgccggta
    12901 cgctgcgtga gaacggtgcg gatctcctca gtgagatcga gtcctttgtc gatgcgattg
    12961 gtgagttgag cgcaggcaac gattgcctgc tcacgctgtt gccgcggcag atttgagagg
    13021 accgtgggaa gcgcggtttg cggggctgca tccggcgtgt aagtcatgag cgcggtcaga
    13081 tacgttggcc aatagccgaa tttctgatac agaccaacat gttttgcgct gtgggcgaag
    13141 gtgaagaggc ccgagtagcg cacaccccag cgatcgaaaa gcttcgttgt tggttcgagt
    13201 agccgttgcg caactccctt gtcccagaat tccggaagaa ccacgagcgg tccgaagaac
    13261 ccgaaggctc cccagcgcgt ggcggcgact accccgatca gacgcgcatc gagacgcgct
    13321 cccagcacct taacgtgctt cgcgcggaag cggggagcca tgaaattgcg atcgcccatg
    13381 aacgccatcg ggtcgggcaa gccgagaaac gtaccgaacg ccaggcgcat aatgcggtcc
    13441 gcttcgggca cttcggattc tttgagggga ctgactttaa tcggcaagcg agcagacata
    13501 gtgtcaaagc ccccattttg gggtattcgg gggacgcggg acagtgtatc gaatgggtgt
    13561 tgtcaattga accccacagc ccgctctgcg gacacagccg cggaaggcag cagagctggg
    13621 agcgcgagcg tctagcgcac gacacccatc tcgtcggccg gccagtagat gaacgtggcc
    13681 ttcccgtaaa tgagttcgcg gtccacggga ccaaaatcgc gcgagtccga tgagatagag
    13741 cgatgatctc ccatgacgaa atactcgtcg gttggaacga tcatctcggg catcgaacgc
    13801 gtgtcgcgat agcgacgggg cacgtacggt tcgtccagag gcgcgccgtt gatccacacc
    13861 cggccatgat cgatgaacac cgagtcgcca ggcagcgcga ccacacgttt gatgtagctc
    13921 ttttcaggat cgcgtggata gtgaaagacc acgacatcgc cgcgagtgat cttttcaaag
    13981 tgaaagaaca gcttgtcgat gaagaggcgg tcctgatcgc gcagttcggg ctgcatgctg
    14041 gtgccttcca cgcgtaccgg ctggtaaagc accgtaacaa tgaagaagga agcgatgacg
    14101 gagatgccga tatcgcgcgc ccacacgcga aattgaaaaa gacgtcgagc ccaggcgcga
    14161 agacggggtt ggaacacggt caggaattcc tcgttgaaat tactctaacc acagtacgca
    14221 tggcatggct aatcggtttc agcgatccca acggctgggg cgcgccggtc acctgccacg
    14281 gccgggatag agcttcacgt gtgcctgagt tcgatacctg cggtaaacgc ggttaactgc
    14341 agcccaaact ccctgtggga cacaggatat gcgccgggcg gccgggtttc ggcagaggct
    14401 gtggctgcgt agaggcaggc acgagcatgg tggggccgtt gaggtttgct tcctgcaatt
    14461 gcaggggacg ctgcagcacc atctcgacct gggtgcctga cgcgatattg acgtcggcgc
    14521 cgcgagtgaa gagggcgacg attccggcgg cggcgagtcc ggcgcccgca ccttcgaggc
    14581 ctccggcgag cggatggccg ccctccagcc ccccgatgga gccaatggta gcgccggtgg
    14641 gcacagccac tttggcggtc tcgccggcat tatgtgcctt attgctgcca ccgtcctgct
    14701 ggatcgtgcc ctcgtcgccg ttgaccttct gatggtcggc gccgggcagg ctgttgacca
    14761 cgccaggaat ctcaaccacc gatccattgg gaaagatcat ggatgtgaag tgcatattga
    14821 gttgcgcctt ttttgccccg attccacggc caggccgaac gacattgtcc accacgccct
    14881 gcacatagac gccggccgga atgacgacgc ggctgcccac gatcaccgga aacgtagagg
    14941 agagatacac gccgtcaccg ggtttggcgc tctttgtatt aacggagctg cgcagttcaa
    15001 gcagcacgcg agttccggca ggaacgagga tcgttgcagg gggtgcggct gcggccactg
    15061 ccggaggagt agcctccgcg gtctgcgttg cagtgggcgc ggctgttgaa ggcattggcg
    15121 ccgacgctgg cgttgcgtcc tgcgccgcgg ccgtgaggct tacagtgaga acaatagcgg
    15181 caagcgtgaa acggacgcgg gtcatcaact gcctccagga aacccacact tattgtgcat
    15241 cgggagcgcg cgtgggtggt gacaattccg aacggtcgga actgctaatt ggaccggaaa
    15301 ggatgctctg gggtgagcag gggtgttcgg aacagaatgt cgccgttagc acagctccat
    15361 tttcacgcaa atgcccttgc aggatgacgt tgcgttcaga attctaagtg ctcgctggtt
    15421 ttgccgagag aatcgcccac aggaaacaca gagcaatgac gatctcaaat atgaccggcg
    15481 ccaggaagaa atagagatac gaattgaccg ctatcgccag atacgccgcc atgaagctcg
    15541 cgaaaactgg gcgcagctgt ggcccgatgg ttttcgccag ggagctgagt tgccagaaga
    15601 cgatcgcctc gatgaacaga aagatcgaaa cggcgagccc cattccgtgg tagtactgcc
    15661 agaacgttcg cgtgactcca agcgcgacaa attggttcga cttcatcgct tcgacggcga
    15721 tgctggcagg tccgggcgga atcgccccga agactccgcc gacggtatgc agcactgcgt
    15781 ggatgaaggt aaggacggag gcaatgcgca gagcgaggac gggcctcatg ccttcgctcc
    15841 tttcgccgct tcgctctcga cgacacgggc atagctgtcg agaaccccaa tccacgcacc
    15901 gggtccatcg aaaagggcga ctaattgctc gtagccctc
//
LOCUS       DASZYN010000019         2978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_450844, whole genome shotgun sequence.
ACCESSION   DASZYN010000019 DASZYN010000000
VERSION     DASZYN010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450844"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            729..2093
                     /locus_tag="VHE33_00525"
     CDS             729..2093
                     /locus_tag="VHE33_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95958.1"
                     /translation="MRTCPREHCNRGSAKSGSRESNGNARPAGSFFFPLDQHLELGTE
                     GYSPAVIRLAVHKAQGFACFKAASLDLKESLDVGMSPKHMERLCRRVGEEWSAQRDRQ
                     VESYQRRQLPRDYPKAAPAAAAVMVDSGRVQTRQENQPPGVYQPAWKATNVACCLTLK
                     SQSHQEDPQPEPPRKFLDPPKVQQLVAQIHARGSGEKPPVGKQTPKRKNKKRRSKESS
                     RKNPNHRKGPVRLVRTTVATMLNWEQFGWQVAAEAHRRSLDLAARKAYVCDGQACNWT
                     LFEMHFQASGFVAILDILHLLGYLYAAALAIAGEKGLWPRYERWLRLAWAGRSGVLLQ
                     ELKSAAGERGVAPRDAAEGDPRRVLAEAVRYVSNNQSRMDYPRYRKLGLPISSAPVES
                     VIKQYNQRIKGSEKFWLPAMVEAMQQIRAAYLCDDGRAEKYWNLPRPHRHAVGGGRLN
                     SAAA"
     gene            complement(2124..>2978)
                     /locus_tag="VHE33_00530"
     CDS             complement(2124..>2978)
                     /locus_tag="VHE33_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95959.1"
                     /translation="PPGVLELSIDVIPPVPWFLNKPFELTVGDHAVQSVFSTAAWTDS
                     WRPLLGKLRDLWQAKQPGRLLVAVAGPPGVGKSVFAEQLNWLVYKQVLHHDAHSMALP
                     MDGFHFPNEYLKTHERKLPDGGAVKLIDVKGQPDTIDVANLRKHLEALRARKDVVDWP
                     GYSRFAHDVLPNKYRIPVITNFVVVEGNYLLVDRGPFAGIPALFDVKIYVDGPAPMIM
                     MKLMERHIKGGKTVEQAKDWVKRIDLPNARIAESTKKSADVVVERDSENELAGIQWRE
                     EEGSAPLP"
BASE COUNT          574 a          832 c         1015 g          557 t
ORIGIN      
        1 ccgcggtctt atccagcttc accagcacct gccgccgaca cgcgatcaat ccgcaactct
       61 atctgacgca gcttctggtc aatctcccgg ccaccccgct cagccagctc ggtgcgtggc
      121 tgccggatca atggaaatca ccaaatcctg ccccgtaatg cccccgcgac atgggtgcgg
      181 ttcacgcagc gctcacaccc ttgtcacatc atgagattgc tgttcaatcc gcaactgcca
      241 aaccctcaac aagaagtaat tccgccacgg tccatctgct gatcatctgt ggtgttgttg
      301 tagttttttt catgcttgcc gccagcatcg tagggggcgt gggtgcatgt ccgtacttgt
      361 atcggtccaa gttgatatga tgagactcag gctgcggcgg tagccgtggg ctcaggaagg
      421 gagctggccg aggatgacaa aggagcgagg aatggaattc cttcaaagca ccgcaggagc
      481 ggaatcggat gcgggattgg cagcattttt ggagaaatcc cggcaggcat gggtggggat
      541 gatgcgggat aaggcgcttg tgacctttga ccagcatgag cagcgggcgg tcgatgaagg
      601 gcgtttgctg gcgcggctgc tgctcgaagc gcgtctggcc aggaatcccg cggcgcgtcc
      661 ggcggagcat gatcagcggc cgtgctgtcc caaatgcggt ctgcccgggg cgccggtggc
      721 tcctgcgcat gcggacctgc ccgagagagc actgcaatcg cggatcggca aagtccggtt
      781 cgcgcgagag caatggaaat gcacgacctg ccgggtcgtt ttttttccct ctggaccagc
      841 atctggaact ggggaccgaa gggtacagtc cggcggtcat ccgcctggcg gtgcataagg
      901 cccagggatt tgcctgcttt aaagccgcca gtcttgattt aaaggaatcc ctggatgtgg
      961 ggatgagtcc caagcacatg gagcgattgt gccggcgggt gggagaggaa tggtcggcac
     1021 agcgggatcg gcaggtggaa agctaccagc ggcggcagtt gccgcgggat tatcccaagg
     1081 cggctccggc ggcggcggcg gtgatggtcg acagcgggcg ggtgcagacg cggcaggaaa
     1141 atcagccgcc gggggtgtat cagcccgcct ggaaggcgac caacgtggcg tgttgtctga
     1201 cgcttaagag ccagagccat caggaggatc cgcagcccga gccgccgcgc aagttcctcg
     1261 atccgcccaa ggttcagcag ctggtagcgc agatacacgc ccgcggcagc ggcgaaaagc
     1321 cgcctgtcgg caaacagacg ccaaagcgta agaacaaaaa gcgtaggagc aaggagtctt
     1381 cacgcaagaa tccaaaccat cgcaagggcc cggtgcgtct ggtgcgcacg accgtggcga
     1441 cgatgttgaa ctgggagcag tttggctggc aggtggctgc cgaagcgcac cggcgcagcc
     1501 tcgatctggc ggctcgcaaa gcgtatgtgt gcgatggcca ggcgtgtaac tggacgctct
     1561 ttgagatgca ctttcaagcc agtggatttg tggcgatcct ggacatcctg catcttctgg
     1621 gctacctgta tgccgccgcc ctggcgatag cgggagaaaa aggcctgtgg ccgcgctatg
     1681 agcgctggct gaggctggcc tgggcgggca ggagcggcgt gcttttgcag gaattaaagt
     1741 cggccgcggg cgaacggggg gtggcgccca gagacgccgc cgaaggcgat ccgcggcggg
     1801 tgttggccga agcggtgcgt tacgtgagca acaaccagtc acggatggat tacccgcggt
     1861 atcgcaagct ggggcttccc atcagcagcg ccccggtcga atcggtgatc aagcaataca
     1921 atcagcggat caaaggcagc gagaaattct ggctgccggc gatggtcgaa gccatgcagc
     1981 agattcgcgc cgcgtatctc tgcgatgacg gccgcgcgga aaaatactgg aatctccccc
     2041 gtccccaccg ccacgccgtc ggcggcggcc ggctgaattc agccgccgct tagactgcaa
     2101 gtacggacat gcacccctct ttttcacggg agcggggcgg agccttcttc ctcgcgccac
     2161 tggatgccgg cgagttcatt ttccgaatcg cgttcgacga caacgtcggc ggatttcttg
     2221 gtggattcgg caatgcgggc gttgggaagg tcgatgcgtt tgacccagtc tttggcctgc
     2281 tcgacggtct tgccgccttt gatgtgccgt tccatgagct tcatcatgat catgggcgcc
     2341 gggccgtcga cgtagatttt cacgtcgaag agggccggga ttccggcgaa ggggccgcga
     2401 tcgacgagca gataattacc ctcgacgacg acgaagttgg tgatcacggg gatgcggtat
     2461 ttgttgggga gcacatcgtg tgcgaagcgg gagtaaccgg gccagtcgac gacgtccttg
     2521 cgggcgcgga gcgcttcgag gtgtttgcgg aggttggcga cgtcgatcgt gtcgggctgg
     2581 cccttgacgt cgatgagctt gacggcgccg ccgtcgggca gcttgcgctc atgggttttg
     2641 aggtattcgt tggggaagtg aaagccgtcc atgggaagag ccatgctgtg ggcgtcgtgg
     2701 tgcaggacct gcttgtagac cagccagttg agctgttcgg cgaagaccga cttgcccacg
     2761 cccggaggac cggcgacggc cacgaggagg cggccgggct gtttcgcctg ccagaggtcc
     2821 cgaagctttc ccaggagagg ccgccacgag tcggtccagg cggcggtgga aaagaccgat
     2881 tgaacggcgt gatcgcccac cgtcagctcg aagggtttgt tcaaaaacca gggcaccggc
     2941 ggtatgacgt caatgctcaa ttcgaggacc cccggagg
//
LOCUS       DASZYN010000020         2812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_129809, whole genome shotgun sequence.
ACCESSION   DASZYN010000020 DASZYN010000000
VERSION     DASZYN010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129809"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VHE33_00535"
     CDS             complement(<1..600)
                     /locus_tag="VHE33_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HVT95960.1"
                     /translation="MASRYNFLETEPKWQSVWAERGSFKTAHDPKLPKYYVLEMFPYP
                     SGRIHMGHVRNYTMGDVVARYKRARGFNVLHPMGWDAFGLPAENAARDQKIHPARWTY
                     DNIATMRAELKRMGLSLDWSRELATCHPGYYKYQQELFLDFFQAGLAYRGEAFVNWDP
                     VDHTVLANEQVIDGKGWRSGAPVEKKKLSQWFLKITAYAD"
     gene            complement(687..1256)
                     /locus_tag="VHE33_00540"
     CDS             complement(687..1256)
                     /locus_tag="VHE33_00540"
                     /inference="COORDINATES: protein motif:HMM:NF023522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3576 domain-containing protein"
                     /protein_id="HVT95961.1"
                     /translation="MKFAAFAGRGAFAKRLISIVLALTALGWLSACNFDIGGSQDYPE
                     QTHQAGKGNYDPNGGTLFGKGSIFSNNTADAPDNGASGGVGVNSLLWRASLDTISFMP
                     LVSADPFGGVIITDWYTPPSTPNERFKVNIYILGRALRADGIRASVFRQTNDTGTWAD
                     APVALNTSTDLENAILTRARQLRMSGVQQ"
     gene            1451..2287
                     /locus_tag="VHE33_00545"
     CDS             1451..2287
                     /locus_tag="VHE33_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HVT95962.1"
                     /translation="MGRIIWMASYPKSGNTWLRAFLHNLLRNPNEAYDINRLGEFSFS
                     DSTIHFYTPYLTKPWNEWTTRDVQEARWKATRDIGRMSPDDVFVKTHNALVEFDDLPM
                     IHMDLTAGAIYIVRNPLDVCISLADHYGVSISEAIRIMAAPTSGTSNSDQLVFEVHNT
                     WSTHVFSWTKQPGPWLHVMRYEDMLKKPIVAFSRVARFLGLNPPRDRIERAIKASSFE
                     SLRAQEDARGFRERSYHAEKFFRVGKANQWREKLTESEIQRVVDNHKVQMERFGYWPI
                     PR"
     gene            2608..>2812
                     /locus_tag="VHE33_00550"
     CDS             2608..>2812
                     /locus_tag="VHE33_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95963.1"
                     /translation="MFGLFCHYDPVLCVRTPHKGLKTGAGKTAERTEQNRPEDEGHPG
                     SVPIPVAIANLGNLEMKKVLLGTT"
BASE COUNT          527 a          859 c          895 g          531 t
ORIGIN      
        1 gtcggcatag gcggtgatct tcaggaacca ctggctgagc ttcttcttct cgaccggcgc
       61 gccggagcgc cagcccttgc cgtcgatcac ctgctcgttg gcgagcacgg tgtggtccac
      121 cggatcccag ttgacgaagg cctcgccgcg ataggcgagc cccgcctgga agaaatccag
      181 gaacagttct tgctggtact tgtaataacc gggatggcag gtcgcgagct cgcgcgacca
      241 gtccagcgac aggcccatgc gcttcagctc ggcgcgcatg gtcgcgatgt tgtcataggt
      301 ccagcgcgcc gggtggatct tctggtcgcg ggccgcattc tcggcgggca ggccgaacgc
      361 gtcccagccc atcggatgca gcacgttgaa gccgcgcgcc cgcttgtagc gcgcgaccac
      421 gtcgcccatc gtgtagttcc gcacatggcc catatggatg cggccgctgg gatacggaaa
      481 catctcgagc acgtagtatt tgggcagctt gggatcgtgc gcggtcttga agctgccgcg
      541 ctcggcccag accgactgcc atttcggctc ggtctccagg aaattgtatc gtgatgccat
      601 gctctgctcg tgggctgctg cccggtgaat taccaggccc gcccccagag aaaaccggac
      661 tggcggccgg tgccggttct actctgctat tgctggacgc ccgacatccg aagctgccgg
      721 gcgcgggtca ggatcgcgtt ctcgaggtcg gtgctggtgt tgagtgccac cggcgcatcg
      781 gcccaggtgc ccgtgtcgtt ggtctggcgg aacaccgagg cgcggatgcc gtcggcccgc
      841 agcgcccggc cgaggatata gatattgacc ttgaaccgct cgttcggggt cgagggcggc
      901 gtgtaccagt cggtgatgat gacgccgccg aacggatccg ccgagaccag cggcatgaag
      961 gagatggtgt cgagcgaggc gcgccacagc aggctgttga cgccgacgcc gccggaggcg
     1021 ccgttatccg gcgcatcggc ggtgttgttg ctgaagatgc tgcccttgcc gaagagcgtc
     1081 ccgccattcg ggtcgtaatt gcccttaccg gcctggtggg tctgttccgg gtagtcctgg
     1141 gagccgccga tatcgaaatt gcacgcgctc agccagccga gggcggtcag tgcgaggacg
     1201 atcgaaatca agcgtttggc aaaggcgccc ctacccgcga aggcggcgaa tttcatgttg
     1261 acggcccttg ctgctcccag ataaagcggc ggtcgctgtt caatgagcgt gtcccgctcg
     1321 gctgcccatg acctaagcga tttgcgcctt tcgggtcaag ggaaaggggg gtcgcgccga
     1381 ccggcgccat agctgcccgg gcagttcccg gattccccgt cccgctcgag gaaagaacgc
     1441 ggttaacgtc atgggccgaa tcatctggat ggcgagctat cccaaatccg ggaacacctg
     1501 gctgcgcgcc tttctgcaca atctgctccg caatcccaac gaggcttacg acatcaatcg
     1561 cctcggggaa ttttccttca gcgactccac cattcacttc tatacgcctt acctgacgaa
     1621 accctggaat gaatggacca cgcgggatgt ccaggaggcc cgctggaagg ccacccgcga
     1681 catcggccgg atgagtcccg acgacgtctt cgtgaagacc cataatgcgc tggtggaatt
     1741 cgacgacctg ccgatgatcc acatggatct gacggccggc gctatatata tcgtgcgcaa
     1801 cccgctcgat gtctgcatct ccctggccga ccattacggg gtcagcatca gcgaggccat
     1861 ccggatcatg gcggccccga ccagcggcac gtccaacagc gaccagctgg tcttcgaggt
     1921 ccacaacacc tggtccaccc atgtcttcag ctggaccaag cagccggggc cctggctcca
     1981 tgtgatgcgc tatgaggaca tgctgaagaa gccgatcgtc gccttttccc gggtggcgcg
     2041 gttcctgggc ctcaatccgc cgcgcgatcg gatcgagcgg gcgatcaagg cctcctcctt
     2101 cgagtcgctc cgggcgcagg aggatgcgcg gggctttcgg gagcgctcct accacgccga
     2161 gaagttcttc cgggtcggca aggccaatca gtggcgggaa aagctgaccg agtcagaaat
     2221 ccagcgggtc gtggacaacc acaaggtcca gatggagcgg ttcggctatt ggccgatccc
     2281 gcggtgacgc gaggggaaat ttaggccgct attcctgttc cggtggcttt agcataaatc
     2341 caacccacta tcactggcgt gtggggaggt tttttcccaa gcggcagtcg ggcgaacgga
     2401 ccggtccgga acgactgtgg cggccggatc cgccggcgcg gaaataaggc aacgccctgc
     2461 cacaatccgt cccgttgaat caggccgtta gcgcccaact gtggcgacgc cgccacagat
     2521 ttggaaaaat gccacaggct ttgcaccaaa tggtttgaca ccaagacagg gtttagagaa
     2581 ggtttcgctg tttccaggtc ggtgggtttg ttcggcctgt tctgccatta cgacccggtc
     2641 ttatgcgtcc gcacgccgca taagggtctg aaaaccgggg cgggcaaaac agcagaacgt
     2701 accgaacaaa atcggcctga ggacgagggg catccgggat cggtaccgat tccggtggca
     2761 atcgcaaact tagggaatct cgagatgaag aaagttctcc tgggtaccac gg
//
LOCUS       DASZYN010000021        12142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_65482, whole genome shotgun sequence.
ACCESSION   DASZYN010000021 DASZYN010000000
VERSION     DASZYN010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 12142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12142
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65482"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="VHE33_00555"
     CDS             <1..1365
                     /locus_tag="VHE33_00555"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HVT95964.1"
                     /translation="RAILSVQYPNGFVPVNAGADDVEVNTPNDGTYDLDPRDFYRYTE
                     TADPYVGRLEYRSPQPHQWGVDQKEGIISVQEKTMLPILGMAAWQVYLTTGDQGFLAE
                     MYRRLSRYDDWFWRRRNTGDGLLVWYNPEESGWDNAARLLPLPVKTVDGSTMAYLLRK
                     MLAASARQLALPDEAAKYEQRAEQTARSINEQMWDDTTGFYYDLSLDGQRRREKSPAG
                     FLPLMAGIVSEDRGKRLAEHLRDAKEFATSAPVPTVSLDDPEYDPRTWGWNGPSWIPS
                     NWLVMESFARAGMVDDSNRIMQAMEEMMSKPDGWPGAYEQYNSQTGMPFGVADYSWSG
                     AINDYLTRWVAGVQPDVPGHALIIAPHLLGEWKSFELDHLRIGHNEIGYRWEENGDRI
                     RMRVNETGPDTLNTELVIHTKQPPHQVLLNGIAMAASTYRIERGDLHIAVPGSGTQTV
                     DVIQ"
     gene            1683..3674
                     /locus_tag="VHE33_00560"
     CDS             1683..3674
                     /locus_tag="VHE33_00560"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95965.1"
                     /translation="MAAPAPYPLGMDIRVDGSSLLSSSAGIRIERQTLDLEHAELVTE
                     MNFTSATGSRLELRVTQFAARSVPSLLCEQVEVTPSKDGDIEIIPQIQHEGVPGTVYR
                     SDVPGGKKEASLVLGMESDRHSRIGEAVVVLPLENLEPRPGGVFRLAVHENRTVILHV
                     VAAIVTSAYDPQPDLQAIRVASWGAMLGWDELRAQNRAAWTDLWRSRLVIDGDPASQR
                     ALDAAFFYLHSSVHRNLLTGVPPFGMSQWADYAGHIFWDMDSWDLPAVVAADPEAARA
                     MVLYRARGLPAAQRKAASFGMEGAMYPWEAGLDGSEQTPSEANTGWAEQHIVPDVAVA
                     AWEYYEATEDKQTLREAVWPILRTVADWIVHRGSVTARGFEIHNVMGHNEWVANVSND
                     SMVNLLCRMALRDAIAAAIAIGEAPRPEWSRIEKTIYIPQDPQRKVIEPFSHDGPLTY
                     FNEPENRFEGVDLAAHPEAYTLNNTQILVFHNPPIASELYRNTWRYEETLRAQRAASP
                     SVPGSVRSPGFSIPPLAACAAMFGERKEAGELFRLAATEYVTGPFDIPKEYRPYRDGA
                     YITNNASLLMAAFYGFTGMRISDGDWRKYPVSLPAGWRRLEIDQVWIKGRAWHVIAEQ
                     GKPLTMLPVPKTRKLPTDRRASQSKTNPSQEEQHAIGLH"
     gene            3655..4923
                     /locus_tag="VHE33_00565"
     CDS             3655..4923
                     /locus_tag="VHE33_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HVT95966.1"
                     /translation="MPSDFIDRRGFLKSALMATATAAIADTTFAAESAAISRITILEV
                     PGEFVRPVAMNAYDKRPVGKSGHIRLVRVFLADGTYGLAVEGYEPIRQPALSFLKSMI
                     GVRPEAVFRWQGERIAGYAPEFAQTMSEKNNCWFELALLDLVGKLRGKPVFELFGDAV
                     RDGIDAYDGSLYFVDVASGRGAEAVGEVAKAIRDDGYRGVKIKVGRPWQWMPDEHGVV
                     RDIEAVIAAREAAGRNMNLMADANNGYEHHHDWAIRFMKETSSSALAWIEEIFPETVS
                     DYTWFHRELQELNADTPVAEGESVRAMDEFRPFLDAGLYRYIQPDMRTCGFSKILYAA
                     DLAASARVNVVAHNWMSELGRIACLHASKIRRNIPLVEDDRYHDLALDASQFEFRDGQ
                     WFLPKKPGWGVDLSPNYEVFARGNAEIIIT"
     gene            5057..7903
                     /locus_tag="VHE33_00570"
     CDS             5057..7903
                     /locus_tag="VHE33_00570"
                     /inference="COORDINATES: protein motif:HMM:NF039503.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5695 domain-containing protein"
                     /protein_id="HVT95967.1"
                     /translation="MQFDDLSAGVMRLDSPGDPYGTNYVIGKEEHPDFSVADSRWFGD
                     VVFRFRAHGQAWQHASTAGSRHARTVEQTGESRGQRVIFHYRSDASSSDGIRGFDFDE
                     SYELEPSTLVWTLTLRNQTGEPLEIGDIGLPLLFNTIYARDPKITYQERVIRHSYIAG
                     DNSFVFWTRANGAGPYLLMTPHSGTQLEFFEPAIPGNHADSTDPVYYPRNAWEGLYTA
                     YIHAAAEGRDYETSGTWRQPLTSKVLAPAGQPGSSITYAFDFRWASTYRDIRQMLYQD
                     GLIDVTAAPGMVIPSDLPALLALHTREPIHSVTAEHPSETEITAVPAPQKDMHVYRIR
                     FRRLGENEIAVSFGNHRRTLLEYDSIEPLDTVIRKRAEFVVDHQQVHDPSKWYDGEFS
                     LWDMRSRILRTPDDRGGLDPYMVGGSDDPDLCKAPYIAAVNEAYPDDRQIAAVEYYLQ
                     HFVWGKLQRTGDERPFPHGIYGSPSWHENRFSATGYDSGGNGREHMWRTFDYTHLIQL
                     YYEMYRIAKLYPERVHYLDAAGYLERAAQTAIAFFRVPYQIRMGAPWDFRGWTDWAWT
                     QGAFHEVFIPDVLNALEAEHRTVEANELRAEWNKKVMYFLYDAPYPYGSEMWFDTTGF
                     ESTQAIAHYAALNGIKPDSGLWRDRNDGRLYAHPRVSQADVENFMDREIQGNLAARGT
                     LETNYYQLGSDIRQGGSSNYLLSYMTQMGGWAILDYGLRFAHGSDRTDALRWGYASYL
                     AGWALVNSGNAQSGYGYWFHGADDDGAAGWGFNPAKYGSTWIPDNHLQARGLWPYDGE
                     IDSGFSGALRSAATIVTRDPVFGLIAYGGVVHERENVVDVELHDGLRQRLELLHLPRP
                     ISIRLEGGGFLGQQQIEHIGQNIRLGLRIESRNGSNDSATIELTGLPPGDGLVFVQDG
                     KKSGAEADRDGTYRASINFGRNRRSAEITIQGAI"
     gene            complement(7918..8844)
                     /locus_tag="VHE33_00575"
     CDS             complement(7918..8844)
                     /locus_tag="VHE33_00575"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVT95968.1"
                     /translation="MEWGACTEHMPHYVFSKNNPYDREAPVELVLPARMPSRAFMDSI
                     RTTAVPEDGLAIWFLGQNGFLLKAHESPLIGIDLYLTNSCARRFADLPFRLDRQLPVF
                     IEPEDLEIDVFCTTHSHQDHADPETLQRLKRTETMQFVGPWESLECYRNCGIPEAQCR
                     LLHPNQRLQLGGGVDLKGTFALPTDATDLNHLGLLFRFTNGITYYNSGDTGECALLSS
                     LLPHNVDICSICINGGFHNLAPMDAAHIVHQIGARVAIPCHYDMMVNNAGDPEMLRTA
                     LHVIGSETPVHVMDYYVPWIYRKHRRAPGAAE"
     gene            complement(8860..9960)
                     /locus_tag="VHE33_00580"
     CDS             complement(8860..9960)
                     /locus_tag="VHE33_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HVT95969.1"
                     /translation="MEENAPGVQIGGQAVIHLIRKPTLSGNEPEFLSATILPGRGMNL
                     FQLTANIPGRGETPLLASPSLKDAALTLDEGPADAHGVNSFAFGGAFLAPYANRIRGR
                     LTPDRMNVATEWNGRRLILPAVWKGKKPGAELHAIHGLILARKTDRVHVEQLTDGQSV
                     TGTMDAGDFDGYWPSRTRLIITVALTGRSLEATIVASNVGQEPEPMGIGWHPYFAFPS
                     GDRSQVRLRIPAADLTEVNNYDDVFPTGNIVTVKGTQYDFEAAGGKALGDIFLDDNFS
                     RLMREDGQVVVDVIDPAAAYGLHIKGLSSDIRTIQVYAPPDKQYAAIEEQFNFADPFS
                     AVWKGRDTGMVTLAPGQSVTWKARLELFIPKV"
     gene            complement(10133..11506)
                     /locus_tag="VHE33_00585"
     CDS             complement(10133..11506)
                     /locus_tag="VHE33_00585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HVT95970.1"
                     /translation="MASAMKAPSQRQGSGLNSVLIRSTIVAALGGLLFGFDTAVIAGA
                     TGALTDLFHLTPGSLGLTVAVALWGTITGALFAGVPSDRFGRRDSLRGLAVLFFFSAL
                     GCAVAWNWTSFVAFRFVAGLAIGGSSVIGPMYIAEIAPAKWRGRLVGFFQFNVVFGIM
                     LAYLSNYLIGTLNMGSAEWRWKLGVSAAPAALFFLMLFGIPRSPRWLVKTGRADEASA
                     VLTAVGDPDVELEIAKISDSIHLELAAGRHRLFSRMYRKPVMLAILIGMFNQLAGINS
                     ILYYLNDIFARAGFTKVSGDLQAVVIGATNLLFTMIAMTVIDKLGRKAMLLAGSVGTA
                     ICLAIVCYIFRTGQHERLLIWPLIGYIAFFAFSQGACIWVYISEVFPNAVRAKGQSLG
                     SFTHWFMTALLSWIFPLLSAHSAAVPFGFFSSMMVLQFFVVLFIFPETKGISLEDMQK
                     KLGIGQG"
     gene            complement(11648..>12142)
                     /locus_tag="VHE33_00590"
     CDS             complement(11648..>12142)
                     /locus_tag="VHE33_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT95971.1"
                     /translation="AAKSVVKAIHEHTPEEWDSVMNANVKAMYWASRHVIPVMIRQNG
                     GLILISGSISGEVGIPTQGAYAPSKGALHQMTRQMAIEYAKYHIRVNTIACGTVDTPI
                     VYESAAASGDPDAYWDMLKRAHPIARIANVEEVSSFYAYMASDLATFFTGSILMMDGG
                     FTAQ"
BASE COUNT         2610 a         3468 c         3577 g         2487 t
ORIGIN      
        1 cgcgcgattc tctcggttca gtacccgaac ggctttgtcc ccgtgaatgc cggcgccgac
       61 gatgtcgagg tcaacacacc caatgacggc acctacgatc tggatcccag agatttctat
      121 cggtataccg aaaccgccga tccctatgtc gggcggctcg aatatcggtc gccgcaaccg
      181 caccagtggg gtgtcgacca aaaagaggga atcatctcgg tccaggaaaa gacgatgttg
      241 cctatcctgg gcatggcggc gtggcaggtc tatctcacca ccggcgacca ggggtttctc
      301 gcggaaatgt accggcgtct gtcacgctac gacgactggt tctggcgccg cagaaataca
      361 ggagacggcc ttcttgtttg gtataacccc gaggagagtg gctgggacaa tgccgcgcgc
      421 ctgcttccgc tgcctgtaaa gacggtggat ggcagcacga tggcctatct gttgcggaaa
      481 atgctcgccg cgtcggcccg gcaactcgct ctgccggacg aggcggcgaa atacgagcag
      541 cgagccgaac agacagcgcg cagcattaac gagcagatgt gggatgatac gacgggtttc
      601 tattacgacc tgagtctgga cgggcagcgg agacgggaga agtcgccggc tggttttctt
      661 ccgctgatgg ctggtatcgt ctccgaagat cgtggcaagc gtctcgcaga gcatctgcgc
      721 gacgcgaaag aattcgcgac ctccgcgccc gtgccgacgg tcagcctgga tgatccggaa
      781 tacgatccgc gcacgtgggg ctggaacgga ccttcatgga ttcccagcaa ctggcttgtc
      841 atggagagct tcgcccgtgc tggcatggtc gatgattcga accggatcat gcaggccatg
      901 gaggagatga tgtcgaagcc ggatggctgg ccgggcgcat atgagcaata caattcgcaa
      961 acggggatgc cgtttggggt cgcagattac tcctggagcg gcgcgatcaa tgactatctg
     1021 acgcgctggg tagctggcgt gcaacccgac gtccccggtc acgctttgat cattgctcct
     1081 catctgctgg gtgaatggaa gtcgtttgaa ctggatcacc tccgcattgg ccacaacgag
     1141 atcggttatc gatgggagga aaacggcgac cgcatccgca tgcgcgtgaa cgagacagga
     1201 ccagacacgc tgaatacaga acttgtcatt cacacgaagc aaccgccgca tcaggtgttg
     1261 ctgaatggga tcgccatggc tgccagtact tatcgcatcg aacgcggcga cctgcacatt
     1321 gctgttccag gaagcggaac gcaaacagtg gatgtaatcc agtgagaatg cggatgaagt
     1381 tctgcggcaa agcggctcgc atcatcgtga tctcgatcgg aatgacaata ttctgtgtgt
     1441 gggccgagtc gcaaagatcc gcaccggcgc cgcttgttgc tactacattc ggcgatgcgg
     1501 ccccgaatcg gccctacatt ccgctggagg ttcctaaaac gccccgcctt cagcctgatc
     1561 actttgccag agcgtatctt gctgacgggt tgctgggcat tcggcccaat ccaaatccgc
     1621 tctctcaatc agaaactgtg gcggcagggt acgttttctc cagtcctgac ggaggatttg
     1681 aaatggccgc tccggcgcct tatccgctgg gtatggacat tcgcgtcgat ggcagcagcc
     1741 tgctgagcag ttcggcagga attcggatcg aacggcagac gctggatctc gaacatgccg
     1801 agctggtgac ggagatgaac tttacttccg ccacgggctc gcggcttgaa ctgcgtgtca
     1861 cacagtttgc cgctcgtagt gtgccgtcgt tgctgtgtga gcaggttgag gttacccctt
     1921 caaaggacgg agatatagaa atcattccgc agattcagca cgagggcgtt cctggaacgg
     1981 tctatcgcag cgatgttccg ggcggaaaaa aagaagcatc cctcgtgctc ggaatggaga
     2041 gcgaccggca tagccgcatt ggagaggccg tcgttgttct tccgcttgaa aatctggaac
     2101 cccggcccgg aggtgtgttc cggctggcag ttcacgagaa ccgaacggtg atccttcatg
     2161 tcgttgccgc aatcgtgacc agcgcatacg acccgcagcc ggatctccag gcgattcgcg
     2221 ttgccagctg gggcgcgatg ttgggctggg atgaattgcg tgcgcagaac cgcgcagcct
     2281 ggacggactt atggaggagc cggctggtta tcgacggaga tcccgcttcg caacgagcgc
     2341 tcgacgcggc cttcttctat ctgcattcca gcgtgcacag gaaccttcta acaggagttc
     2401 cgccctttgg aatgagccag tgggcggatt acgccggcca tatcttctgg gacatggaca
     2461 gctgggatct gccggccgtg gtggccgccg atcctgaggc ggcgcgggcg atggtgctct
     2521 accgggcgcg cgggttgccg gcggcgcagc gcaaggcagc cagcttcggc atggaaggcg
     2581 ccatgtatcc gtgggaggcc gggctggatg ggtcggagca gacgccttcg gaggcgaata
     2641 ccgggtgggc tgagcaacac atcgtgcccg atgtagccgt ggccgcgtgg gaatactacg
     2701 aggcaaccga ggacaagcag acgctgcgcg aggccgtgtg gccgatcctg cgcacggtcg
     2761 ccgactggat tgttcaccgg ggctcggtta cggcgcgagg gttcgagatt cacaatgtca
     2821 tgggtcacaa cgagtgggtt gccaacgtgt ccaacgactc aatggtgaat ctgctctgcc
     2881 gtatggcact ccgcgatgcg atcgcggcag ccatcgcaat cggtgaggca cctcgcccgg
     2941 agtggtccag gatcgagaag acgatctata ttcctcagga tccacagcga aaggttatcg
     3001 aaccattcag ccacgatggt ccgctgactt acttcaatga gccggagaac aggttcgaag
     3061 gagtggacct ggcagcacat cctgaggctt acacgctgaa taacactcag attctggttt
     3121 ttcacaaccc tcccatcgcg tccgagcttt atcggaatac ctggcgctat gaagaaaccc
     3181 tgcgagcgca aagggcggcg tctccgagtg ttcccggcag cgtgcgatcg ccagggttct
     3241 ccatcccgcc gctcgctgcc tgtgcggcga tgtttggaga aagaaaggaa gcgggagagc
     3301 tatttcgtct agctgccact gagtatgtca ccggcccgtt cgatatccca aaggaatacc
     3361 ggccctaccg ggacggagcc tacatcacaa ataacgcgtc actgttgatg gctgctttct
     3421 acgggttcac gggaatgcga atctcggacg gtgattggcg aaagtaccct gtaagtctgc
     3481 cggccgggtg gcgacgcctg gagatcgacc aggtctggat caaaggacga gcatggcacg
     3541 tcatagcaga gcaggggaag ccgttgacga tgcttcccgt tccaaagacg cggaaactgc
     3601 ccaccgatcg acgggcgagc caaagtaaaa ccaacccgag ccaggaggag cagcatgcca
     3661 tcggacttca ttgatcgcag aggattcctg aagtcggctc tgatggcgac cgccactgcc
     3721 gccattgcgg atactacgtt cgccgcagaa tctgcggcta tcagcaggat taccattctt
     3781 gaggtccccg gtgagtttgt tcggcccgtg gccatgaatg catacgataa gcgcccggta
     3841 gggaagtcgg gacacattcg tctggtccgc gtatttttag cggacggcac atatgggttg
     3901 gccgtggaag gctatgagcc gatccgccag cccgcgctca gcttcctgaa aagcatgatt
     3961 ggggtcaggc cggaggcggt ctttcgatgg cagggcgaac gcatcgccgg atacgctccg
     4021 gagtttgccc agaccatgtc agagaagaac aactgctggt tcgaactcgc tttgctcgac
     4081 ctggtaggca aacttcgcgg taagccggtc tttgagctct ttggcgatgc tgtgcgggac
     4141 ggaattgacg cttatgacgg gtcgctttac ttcgtggacg ttgcaagcgg ccggggggct
     4201 gaggcggtcg gcgaagtggc caaagccatc cgtgacgatg gttatcgagg cgtgaagatc
     4261 aaggtgggcc gtccatggca gtggatgccg gacgagcacg gagtggtgcg agacatcgag
     4321 gcggtaattg cagcgcgaga ggctgccggc cggaacatga acctgatggc cgatgcgaac
     4381 aatggatacg aacatcatca cgactgggcg atccggttca tgaaagaaac ttcgtccagc
     4441 gctcttgcct ggatagagga aatttttccg gaaacggtga gcgattacac atggttccat
     4501 cgcgaactgc aggagttgaa cgcggatacg ccggtggctg aaggcgaaag cgtccgagcg
     4561 atggatgaat tccgtccatt cctcgatgca ggactgtacc gatacatcca gccggacatg
     4621 cgaacctgcg gcttctcgaa gatactttat gcagctgatc tggctgcatc agcacgtgtg
     4681 aatgtggtcg ctcataactg gatgagcgag ttaggcagga tcgcctgcct ccatgcgtcg
     4741 aagatccggc gaaacatccc cctggttgag gatgatcgct accacgacct tgctctcgat
     4801 gcttcccagt tcgaatttcg cgatggccag tggtttcttc cgaagaagcc cggctggggt
     4861 gttgatctca gccctaacta cgaggtgttt gcgcggggca acgcagagat catcattacc
     4921 tgaggctaaa cggatgcgat cgcaggccgc gagaatttct atcggattat tgtgtgcggc
     4981 aattctgtcg gttccggcgt ctgccgttgc agcgtctccg tgcagcggaa ggcatctgaa
     5041 gaacgagacc ttctgcgtgc agtttgacga cctgagcgcc ggcgtgatgc ggctcgattc
     5101 gcccggtgat ccatacggga cgaactatgt gatcggcaaa gaggagcatc cggacttttc
     5161 ggttgcggat tcacgctggt ttggcgatgt agtgtttcgc tttcgagctc acgggcaggc
     5221 atggcagcat gcgagtaccg cgggctcaag acatgcaaga actgtcgagc aaacgggtga
     5281 gtcccgagga cagcgtgtca tatttcacta tcgcagtgat gcgtcgagca gcgatggaat
     5341 ccgcggtttc gatttcgatg agtcgtacga gttagaacct tccactctcg tgtggaccct
     5401 tactctgcgg aatcagacag gcgaaccgct ggaaatcggc gacatcgggc ttccacttct
     5461 cttcaacacc atatacgcgc gcgatccaaa gatcacttat caggagcgcg taattcgaca
     5521 tagttatata gcgggagaca attccttcgt cttctggaca cgggcgaatg gggcaggacc
     5581 ctatctgctc atgacgcccc attcgggaac gcagctggaa ttctttgagc ctgcaattcc
     5641 cggcaaccat gctgactcca ccgaccccgt ctactatccg cgtaacgcat gggaaggcct
     5701 ctatacggca tacatccatg ctgccgcaga aggaagagac tatgaaacgt cgggaacctg
     5761 gcgacagccg cttacttcca aagtgctcgc accggctggt cagccgggtt cgtcgatcac
     5821 ctacgccttt gactttcgct gggcatcgac ctaccgcgat atccggcaaa tgctctatca
     5881 ggatggcctg attgatgtca cggcggcgcc agggatggtg atcccatcgg acctgccggc
     5941 tctgctggcg ctgcatacgc gtgagccgat ccatagtgtc acggctgagc acccctcaga
     6001 aaccgagatt acggcggtcc cggccccgca gaaagatatg catgtctacc gcattcgctt
     6061 ccgccgccta ggggaaaatg aaatcgcggt aagcttcggt aaccatcgca ggacgctgct
     6121 ggaatatgac tcgatcgagc ccctcgatac agtcatccgc aagcgagcgg agtttgtggt
     6181 ggatcatcag caggtacacg atccgtccaa atggtacgac ggtgagtttt cattgtggga
     6241 catgcgtagc cgtatcctgc gcacgccgga tgatcgcgga gggctggatc cctacatggt
     6301 tgggggatcg gacgatccag acctgtgcaa ggcaccttac attgcggccg tgaacgaggc
     6361 ttatcccgac gaccgccaga ttgcggcagt ggagtattac cttcagcatt ttgtttgggg
     6421 aaagctgcaa cgcactggag atgagcggcc ctttccccac ggtatttacg ggtccccgag
     6481 ctggcatgag aaccgcttca gcgcaaccgg atacgattcc ggcggcaatg gccgggagca
     6541 tatgtggcga accttcgatt acacacatct gatccagctc tattatgaga tgtaccgaat
     6601 tgcaaaacta tatccggagc gtgtgcacta tctcgacgca gccggttatc tggaacgagc
     6661 cgcgcagaca gccattgcat tctttcgcgt gccgtaccag attcgcatgg gagcgccctg
     6721 ggactttcga gggtggacgg attgggcctg gacgcagggg gcgttccacg aagtcttcat
     6781 tcccgacgtg ttgaatgcac tggaggccga gcaccgcacc gttgaagcaa acgaactgcg
     6841 agcggaatgg aataagaagg tgatgtactt tctgtatgat gccccgtatc cctacgggag
     6901 cgaaatgtgg ttcgatacga ccgggtttga atccacgcag gccatcgctc actatgccgc
     6961 cctcaacggg atcaagccgg acagcggtct gtggcgcgac cggaacgacg gaaggctgta
     7021 tgcccacccc cgtgtaagtc aggctgatgt cgaaaacttt atggaccggg agattcaggg
     7081 gaatctggcc gcacgcggca cactggagac aaactactac cagctgggaa gcgatatccg
     7141 gcagggaggc agctcgaatt atctgctcag ttatatgacc caaatgggtg gttgggcgat
     7201 cctggattat ggcctgcggt tcgcgcacgg ctcagaccgc accgatgcac tccggtgggg
     7261 ctacgcgtcg tatctggcag gctgggctct ggtcaactca ggcaatgctc agagcggcta
     7321 cggctactgg ttccatggcg cggacgatga tggcgcggca ggatggggat tcaatccggc
     7381 gaaatacggg agtacctgga ttccagataa tcaccttcag gctcgaggtt tatggcccta
     7441 cgacggggag attgacagcg gtttctccgg tgcgcttcga tccgcagcga ccattgtcac
     7501 gcgcgatcca gtcttcggcc tgatcgctta tgggggcgtg gtgcacgaac gggagaacgt
     7561 tgtggatgta gagttacacg atgggcttag acagcggctc gagcttctgc acttgccgcg
     7621 gccgatttcg atccggcttg agggtggagg atttctcggt cagcaacaga tcgagcatat
     7681 cggacagaac attcgcctgg ggttgcgaat cgaatcgcgc aacggcagca acgactctgc
     7741 gacgatagaa ctcactggct taccgccggg cgacggtctg gtcttcgtcc aggatggaaa
     7801 gaagtctggg gcagaagccg accgcgatgg cacttaccgc gcgtcgatca acttcggaag
     7861 aaatcgtcgc tccgccgaaa ttacaatcca gggagccatt taggtctgtt cggcgtgtta
     7921 ttccgcggcg ccgggcgccc ttctgtgctt cctgtatatc cacggcacgt agtaatccat
     7981 cacgtgtacg ggagtttccg aacctataac atggagcgcc gtcctcagca tctcagggtc
     8041 gccggcatta ttcaccatca tgtcgtagtg gcagggaatc gccactcgtg ccccgatctg
     8101 gtggacaatg tgcgcagcat ccatgggcgc cagattgtga aaaccgccat tgatgcagat
     8161 cgaacagata tccacgttgt gcgggagcaa ggaggacagc agcgcgcact cacctgtgtc
     8221 gccggagttg taataggtga ttccgtttgt gaaccggaag agaagtccca gatgattcag
     8281 gtcggtggcg tcagtcggca gcgcaaatgt ccccttcagg tcgactcctc ctcccagttg
     8341 cagtcgctga ttcgggtgta acagccggca ttgagcctca gggattccgc agttccggta
     8401 gcattccaac gattcccatg gccccacaaa ttgcatcgtc tcagtccgtt tcagcctttg
     8461 aagagtttcc ggatcggcat ggtcctggtg cgagtgcgtc gtacagaaga cgtcgatctc
     8521 cagatcctcc ggttcaatga acaccggcag ttgccgatcc agacgaaacg gcaaatctgc
     8581 aaatcgccgt gcgcagctgt tggtgagata cagatcgatt ccgatcaaag ggctttcgtg
     8641 cgccttaaga agaaacccat tctgccccag gaaccagatc gccagcccat cctccggaac
     8701 cgccgtcgtt cgaatggaat ccatgaatgc ccggctgggc atgcgtgcag gcagcaccag
     8761 ctccaccggc gcctctcggt cgtatgggtt atttttgctg aatacgtaat gcggcatatg
     8821 ttcggtgcag gccccccact ccacgagcaa tacaccacct taaaccttcg gtatgaacag
     8881 ctccagccga gctttccagg tgacggactg acccggagca agcgtcacca ttccggtatc
     8941 gcggcccttc cagacagcac tgaacggatc tgcgaaattg aattgttcct caatggccgc
     9001 atactgttta tccggtggcg catagacctg gattgtccga atatcgctgg agagtccttt
     9061 gatgtgcaat ccataagccg ccgcgggatc gatgacgtcg accaccacct gtccatcctc
     9121 gcgcatcagc cgtgagaaat tgtcgtcgag aaagatgtct cccagggctt tccctcccgc
     9181 cgcttcgaag tcgtattgtg tgcccttcac cgttacgatg tttccggtag ggaagacatc
     9241 gtcatagttg ttgacttccg ttaaatcggc cgctggaatc cgcagtcgca cctgggaacg
     9301 gtccccgctg ggaaatgcaa aataaggatg ccatccgatt cccatcggct cgggctcctg
     9361 ccccacgtta ctcgcgacga tcgtggcttc gagggaccgc cccgtcagcg ccaccgtaat
     9421 gatcagtcgc gttctggatg gccagtaccc atcaaaatcc cctgcgtcca tcgtccccgt
     9481 taccgactgg ccatccgtca gctgttccac atgcacacgg tcggtttttc gcgcaaggat
     9541 gagtccgtga atcgcatgta gctccgcgcc gggcttcttg cctttccaaa ccgccggcaa
     9601 gatcagcctc ctgccgttcc actccgttgc cacgttcatt cgatccggcg tcaacctgcc
     9661 gcggattcgg ttcgcgtagg gcgccagaaa cgctcctccg aacgcgaacg aattcactcc
     9721 gtgcgcatcg gcaggcccct cgtcgagtgt gagcgcggcg tctttcagcg atggcgaagc
     9781 gagaagagga gtctcacccc tgccgggaat gttcgccgtg agctgaaaca gattcattcc
     9841 acgtcccggc aaaatcgtgg ccgacaggaa ctccggttcg ttgcctgaca gagtcggctt
     9901 tcgtatcaga tgaatgactg cctggccacc gatttgcacc ccgggcgcgt tttcttccat
     9961 agtttccctc gactctgcgg cgacaaccgc gcctgccggc aacgacgacc gtttttctgc
    10021 agccaacaac gaaaagacgc cgattgcgac aagcgcatac ttcaaactga cgcggaatgt
    10081 cgaatgcgtc cgtgtatctc cagaagcgcc atcggtccaa cttttccctc gatcaaccct
    10141 gcccgatgcc cagctttttc tgcatgtcct ccaacgagat tcctttggtc tccgggaaaa
    10201 tgaacaggac cacgaagaac tgaagcacca tcatgctgga gaaaaatcca aacggcaccg
    10261 cggcggagtg agcgctcagc agtgggaaga tccatgagag cagggccgtc atgaaccagt
    10321 gcgtgaagct ccccaggctc tggcctttcg cccggactgc atttggaaag acctcgctga
    10381 tgtacaccca gatgcacgcg ccctgggaaa atgcgaagaa cgcgatgtag ccgatcaacg
    10441 gccaaatcag caaacgctcg tgctgtccgg tgcgaaatat gtagcaaaca atcgccagac
    10501 aaatcgcggt tcccaccgag ccggcgagca acattgcctt ccggcccagc ttgtcgatca
    10561 ccgtcatggc gatcatggtg aacagcagat ttgtcgcgcc aatgacaacg gcctgcaaat
    10621 ctcccgaaac cttcgtgaag cctgctcgag caaaaatgtc gttcaggtaa tacagaatcg
    10681 agttgattcc cgccagctga ttgaacatgc cgataagaat tgccagcatc accggcttgc
    10741 ggtacatgcg cgaaaacaat cgatgccttc ccgcagcgag ttccaaatgg atcgaatccg
    10801 agattttcgc aatctcgagt tcaacgtcag gatcgccgac cgccgtcaat acagccgagg
    10861 cctcgtccgc acgccctgtc ttgaccagcc agcgcgggct gcgcgggatc ccgaacagca
    10921 tcaggaagaa gagtgcggcc ggcgcagccg agacgccgag cttccagcgc cattccgccg
    10981 atcccatatt caacgtgcca atcagatagt tcgacaggta ggcgagcatg attccgaaca
    11041 cgacgttgaa ctggaagaat ccgaccagcc gcccgcgcca cttggccgga gcaatctccg
    11101 ctatgtacat tggcccaatc accgaagaac caccaatggc gagtccggcg acaaatctga
    11161 acgccacgaa cgaagtccag ttccatgcca ccgcgcagcc cagcgccgaa aagaaaaaga
    11221 gaacggccag cccacgcagg ctgtcgcgcc gcccaaatcg atcgctcgga actccggcaa
    11281 acagtgcgcc cgtgatcgtg ccccacagcg ctacggccac ggtgagtcca agcgagccgg
    11341 gtgtcaggtg aaacagatcg gtgagtgcgc cggtcgctcc ggcaatcacc gcggtgtcga
    11401 acccaaagag caggcctccc agcgcggcga cgattgtgct gcggatcagt actgaattga
    11461 gcccgcttcc ctgtcgctgg gagggggcct tcatagcgct ggccattttc ggctccttga
    11521 gcagtcaacg cccagcacgc tctaaattcg gctgagccac cgtgagggag ttatagatta
    11581 tagattcagg agtagtcaag ggatttgcct cctgccgtga cacattcatg gctggatgtc
    11641 ctcggagcta ctgcgccgtg aatccgccat ccatcatcag gatggagcca gtgaaaaatg
    11701 tcgccagatc gctggccata tacgcgtaga aggaagaaac ctcctcgacg ttcgcaatcc
    11761 gggcgatcgg atgagccctc ttcagcatgt cccagtaggc atccggatcg ccggaggccg
    11821 ccgccgattc atacacaatt ggtgtgtcca cggtcccaca ggcaatcgtg ttcacgcgga
    11881 tgtgatattt cgcgtattcg atcgccatct gacgggtcat ctggtggagg gcgcccttcg
    11941 acggcgcgta cgctccctgc gtcgggatcc ccacctcgcc cgagatcgat ccgctgatca
    12001 gaatcaggcc gccattctgg cggatcatta ccggaatcac atgccggctc gcccagtaca
    12061 tggcttttac attggcgttc atcaccgagt cccactcctc gggagtgtgc tcatgaatcg
    12121 ctttcaccac gcttttggcg gc
//
LOCUS       DASZYN010000022         2742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_258170, whole genome shotgun sequence.
ACCESSION   DASZYN010000022 DASZYN010000000
VERSION     DASZYN010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258170"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VHE33_00595"
     CDS             <1..214
                     /locus_tag="VHE33_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95972.1"
                     /translation="NVLNFGNFGGPDGVVLTPDDNGAGLTNVNSPYGTGAAAFDIKNT
                     ERTPRRTGTFGQGSPRATEFQLTFNF"
     gene            707..>2742
                     /locus_tag="VHE33_00600"
     CDS             707..>2742
                     /locus_tag="VHE33_00600"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVT95973.1"
                     /translation="MKQPRLHWIIPVVAALFCCCTCARPALGQATNTGTVVGEVDDQS
                     GAVIADASVTLSNASAGTKLTTSTNGTGKYIFQAVPPGTYDVIVTKSGFSTAKLTGLT
                     VNVGSQATENVKMAVGSTAQTVEVQVMGTELQTLNSTVGQTISQEAIESLPSLNHDVN
                     TFTALQPGVSPGGNVAGAVSDQNMFLLDGGNNSSDMDGNNTVYNTTAAFAGDPTGGQG
                     TPPSGIMPTPSDSVDEVKVNTANQTADFDNSSGAQVEFVTPRGTNRWHGAAYEYYLDN
                     GFNANSWDNNDNVPKTKIPQYHYSRFGGRAGGFFLPSMLGGRWYIFGFYEGFRYPQST
                     TIARIVPTDTLKAGTVYQNGTPYNMTTVDPRGLGIDPVVQQMWTKYEPAGNVGAVGTS
                     AACSGFNTKYCDGFNTVGFKANMSLPLSSNNMTFRIDHSFNSKWNWFASYRYFKLKQF
                     ANKQYDIGGFFSGDKLGVPTSTAPRPLVPSFLVTGLTTNITANITNDFHYSYLRNFWQ
                     WGTNNAPPQIAGLGGAMEPFGESSGNALLPFNVDAQDIRTRFWDGQDHFFSDNVTMLK
                     GNHLIQFGGQYQRNFDYHSRSDNGGGINFTTTYRMGEQSGTGVGGINMSDPVDGLQGG
                     YPISTASVRLAAAVLGIVDQSQVAYTRSGDLSLNPPLTQAFDKDVIPYYNGYAS"
BASE COUNT          577 a          921 c          691 g          553 t
ORIGIN      
        1 caacgtcctg aatttcggca acttcggcgg tcccgatggg gtcgtcctca ccccggatga
       61 caacggggcg ggcctcacca acgtcaacag cccttacggc accggcgccg cggcgttcga
      121 tatcaagaac actgaacgta cgccacgccg caccggcacc ttcggccagg gttccccgcg
      181 cgccaccgaa tttcagctga ccttcaactt ctgacttttc ggtaccactg atacagggcg
      241 gagaagccat tctccgccct cctttttccc cgacagccac agtccccggg gctctcgctc
      301 gtccctatcc cattcgtttt gttcaccctt agacgctgcc cgagactaaa ggtgatgtgt
      361 atttcagaag agagtttgtt gaataactat gaatttgtgt agccattttg tccacaatcg
      421 agcccctaca gtcactaact ctatgattcc aaagtgtcat tgaaatgtcg caaaatgtga
      481 cgaatggccc gccaattgct catgtcaggc ccatcatcat tcttccccgg ctcttgttcg
      541 ctgttttctc cccagtcgta gaaaccagtt cgtagttggt tcaaacgagg aaacgcacga
      601 agccgcctct ctgttgaagc ggttatccgc atccgggcac tccccacgca atcgacccgg
      661 tgatcactaa tttttggcaa cgagtgaagt tcatggagga gctgtgatga agcaacccag
      721 gttgcattgg atcatcccgg tggtcgccgc actgttttgt tgttgtacat gcgcgcgtcc
      781 tgcactgggc caggcaacaa acacaggaac cgtcgtcggc gaagtcgacg atcagagcgg
      841 cgctgtcatt gccgacgcgt ccgtcaccct cagcaatgca tccgctggca ccaaactgac
      901 gacctctacc aacggcaccg gaaagtacat tttccaggcc gtgccgcccg gcacctacga
      961 cgttatcgtc accaagtcgg gcttctcgac ggcgaaattg accggtttga cggtcaacgt
     1021 tggctcgcag gccacagaaa acgtgaagat ggctgtcgga tccacggccc agaccgtcga
     1081 ggtccaggtc atgggcactg agctgcagac cctgaactcc acggtcggcc agaccatctc
     1141 ccaggaagcg attgagtcgc tgcccagcct gaaccacgat gtgaatacgt tcaccgctct
     1201 gcagcccggc gtgagccccg gcggcaacgt ggccggcgcc gtgagcgacc agaatatgtt
     1261 cctgctggat ggcggcaaca attccagcga catggacggc aacaataccg tctacaacac
     1321 caccgccgcc tttgccggcg atcccaccgg cggacagggc acgcctccct ccggcatcat
     1381 gcccacccct tccgacagcg tcgacgaagt caaggtgaac accgccaacc agaccgccga
     1441 cttcgacaac tcctcgggtg cgcaggttga gttcgttacc ccccgcggca cgaaccggtg
     1501 gcacggcgcc gcttacgagt actacctcga taacggcttc aacgccaatt cctgggataa
     1561 caacgacaac gtccccaaga ccaagattcc ccagtatcac tacagccgct tcggcggaag
     1621 ggccggcggc ttcttccttc cctccatgct cggcggtcgc tggtacattt tcggtttcta
     1681 tgagggcttc cgctatcctc agtccaccac catcgccaga atcgttccca ccgacacgct
     1741 gaaagccggc acggtctatc agaacggtac gccctacaat atgaccaccg tcgatccgcg
     1801 cggcctcggc atcgaccccg ttgtccagca gatgtggacc aagtacgagc ccgcgggcaa
     1861 cgtcggcgcg gttggcactt cggcggcctg cagcggtttc aacaccaaat actgcgatgg
     1921 cttcaacacg gtcgggttca aggccaatat gtcgctgcct ctcagcagca acaatatgac
     1981 cttccgcatc gatcactcct ttaactccaa atggaactgg ttcgccagtt atcgctactt
     2041 caagctgaag cagtttgcca acaagcagta cgacatcggc ggcttcttct ctggcgacaa
     2101 actgggagtt cccacctcca cggctcctcg cccgctggtt ccgtccttcc tggttaccgg
     2161 cctcaccacc aacattacag ccaacatcac gaacgacttc cactacagct atctgcgcaa
     2221 cttctggcag tggggaacca acaacgctcc cccacagatc gctggcctcg gcggcgccat
     2281 ggagcctttc ggtgaaagct ccggtaacgc tctgctgcct tttaacgtcg atgcccagga
     2341 cattcgcacc cgcttctggg atggccagga ccacttcttc agcgacaacg tcaccatgct
     2401 gaagggcaat cacctgatcc agttcggcgg ccagtatcag cgcaacttcg actaccactc
     2461 ccgctccgat aacggtggcg gcatcaactt caccaccacc taccggatgg gcgaacagag
     2521 cggcaccggt gtcggcggca tcaacatgtc cgatccggtg gatggtctgc agggtggcta
     2581 tccgatctcc acagcgtccg tgcgtctcgc cgccgctgtc ctcggtatcg tggaccagtc
     2641 ccaggtcgct tacactcgct ccggcgacct gagcctcaac ccgccgctga cccaggcgtt
     2701 cgacaaggac gtcattcctt actacaacgg ctatgccagc ga
//
LOCUS       DASZYN010000023         4218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1229, whole genome shotgun sequence.
ACCESSION   DASZYN010000023 DASZYN010000000
VERSION     DASZYN010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..1165
                     /locus_tag="VHE33_00605"
     CDS             227..1165
                     /locus_tag="VHE33_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVT95974.1"
                     /translation="MTPTRVAASLCLAAFAAIASAQAPTLHVETHLVTLTFTVRDESG
                     KLVPALNAGDFTVYEDGAPQIISIFAHESDLPLHLGLLVDASASQDKFIRQHLRDIHT
                     FLGSVLEPRDQVFALCFGDHLRIAAQPTSDPSAVIDGLDRYNKEYRSFPELDPDDSRD
                     GGTALYDAIVAGTTHELAAHSDGRKALLLFTDGEENSSGHDEIDAIASAQDSDALVYA
                     IRYTEMHHGKLSADNRHGIAALHHLAEQTGGRDFDGLKSDLPQVFRQIGDELRSLYVI
                     GYYSTNKAHDGSFRKVVVVPHTDGLNVRAKSGYYAK"
     gene            complement(1192..2415)
                     /locus_tag="VHE33_00610"
     CDS             complement(1192..2415)
                     /locus_tag="VHE33_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95975.1"
                     /translation="MLSSFRAGWISAVLCVTLSPLGAMRARAEQVDTNPLNREHDVQE
                     TFRHFYNMDYDEALPGFEKIREEHPGNPMATAYLLNATLFRELNRLDLLDTTFYANDG
                     FLTGKHTVVEDPKVHDRIGELSTQAIDEANAELKTNPNDVNALFARGWVKSMQAVYSA
                     MIERAFSGGLRQALGARNDCDRVLELDPHYIDAKLVTGVYQYVVGSLNIAFKIIVGIV
                     GIHGSKDNGLALLRDVGENGVITRVEARTSMMLFLRREAKYDAAVAIAKQMAEEYPHN
                     YLFLLEEANLEKDAGDGMVAVATYQRLIELAKHPGYFPNAHMELTYFGLGDSLRGQRR
                     YSEAVDAYREGALQSTTSPELKRRCLLEAGKTYDLMREHDKAVEEYRAVLSAGRDTAQ
                     GELARKYIQSAYSGH"
     gene            2504..3064
                     /locus_tag="VHE33_00615"
     CDS             2504..3064
                     /locus_tag="VHE33_00615"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HVT95976.1"
                     /translation="MLEWIALVELLLCWIVWAMAFIRPRREAADRKEVAKSGASVWGI
                     GLVMAAYALTFTYVRPRGFHKTAPALIAGMILAPPCVVLAWTAASHLGRQWRYAAALR
                     EDHELIQTGPYRRLRHPIYASMFGMLLATGLCWTWWPQFAVAVVFFVIGTEIRVRSEE
                     RLLESHFGDRYRDYRARTDAWIPFLR"
     gene            3406..3921
                     /locus_tag="VHE33_00620"
     CDS             3406..3921
                     /locus_tag="VHE33_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95977.1"
                     /translation="MTRQELLRIVVLRARVHGFEFRHWFQTNLETEWPGFDQAVSLLC
                     SGRRYYALLFSHDFARHFWKKGTQITFLLPSQEYTRLDSNGKVVTIKRKSFTRRSSRS
                     NSNNVWQYHLEQMAVWDEPLRYIRRFILTDEELEAAGESAPQVVEAPEPAPVKSAETK
                     MQVAPRRADWC"
     gene            4047..4123
                     /locus_tag="VHE33_00625"
     tRNA            4047..4123
                     /locus_tag="VHE33_00625"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4081..4083,aa:Arg,seq:acg)
BASE COUNT          789 a         1378 c         1191 g          860 t
ORIGIN      
        1 gtgagcacag caacaaagat ccgcgtcgtc gggctgctga tcaaggaccc cacctccggg
       61 aactccgtgc tgctggccca ctacatcgac gacttggaca aggactagac tcctccccgg
      121 cgaacccacg aaaggcggcg cactcgaagc tgaggcgccg cctttgattt tagggaaccg
      181 gcgcccgcat ggcggcgtat gggtctttac cccctccgga gaccgcatga ccccgacccg
      241 cgttgccgca tcgctgtgcc tggccgcatt cgccgcgatc gcctccgccc aggcccccac
      301 gctgcatgtc gaaacgcacc tggtgacgct caccttcacc gtgcgcgatg aaagcggaaa
      361 gctggtgccc gcgctgaatg ccggcgactt taccgtctac gaagatgggg cgccgcagat
      421 catcagcatc tttgcccatg aatccgatct gccgctgcac ctgggattgc tggtcgacgc
      481 gtccgcgagt caggacaaat tcatccgcca gcatctgcgc gacatccaca ctttcctggg
      541 cagtgttctc gaaccgcgcg atcaggtctt cgctctttgc tttggcgatc acctgcgcat
      601 cgccgcgcag ccgacctccg atccgtccgc ggtgatcgac ggcctcgatc gctacaacaa
      661 ggaatatcgt tccttccctg aactcgatcc ggacgacagc cgggacggcg gcactgcgtt
      721 gtacgacgcc attgttgcgg gaacgacgca cgagctcgcc gcgcattccg acggccgcaa
      781 ggcgctcctg ctcttcaccg atggcgagga aaactccagc ggtcacgacg agatcgacgc
      841 aattgcaagc gcgcaggaca gcgatgcgct cgtctatgcg atccgctaca ccgaaatgca
      901 tcacggcaaa ctcagcgccg acaatcgtca cggtatcgcg gcactgcatc atctggcgga
      961 acagacgggc ggccgcgatt tcgacgggct gaagagcgac ctgccccagg tcttccgcca
     1021 gattggcgac gagctgcgct cgctctacgt gatcggctac tactcgacga acaaagcgca
     1081 tgacggcagc tttcgcaagg tcgtcgtggt gccgcacact gacggtctca atgtgcgtgc
     1141 gaaatccggg tactacgcga agtaggcgtc ccgggctccc tcaccgctcc atcagtgccc
     1201 gctgtacgcc gactgaatgt atttccgcgc cagttcgccc tgggcggtat cccttccggc
     1261 gctgagtacc gctcgatact cttcgacagc cttgtcgtgc tcccgcatca gatcgtacgt
     1321 ttttccggct tcgagcaggc agcgtcgctt cagctccggg ctggtcgtgg actgcaacgc
     1381 cccctcgcga tacgcgtcca ccgcctcgga atagcgtctc tgtccgcgca ggctgtcgcc
     1441 cagtccaaag tacgtcagct ccatgtgcgc gttgggaaaa tagcccggat gctttgccag
     1501 ctcgatgagg cgctgatagg tagccactgc gaccatgccg tcgccggcgt ccttttcgag
     1561 gttcgcttct tccagcagaa acaaataatt gtgcggatac tcttccgcca tttgctttgc
     1621 gatcgcgacc gccgcatcgt acttcgcctc gcgccgcagg aacagcatca tggaagtcct
     1681 ggcttcgacc cgcgtgatga cgccgttctc gccaacgtcg cgcagcaggg ccaggccgtt
     1741 gtctttggaa ccgtgaatcc ccacgatgcc gacgatgatc ttgaaggcga tgttgaggga
     1801 gccgacgaca tactgataga cgcccgtgac cagcttcgca tcgatgtaat gcggatcgag
     1861 ctcgagcacg cggtcgcagt cgttgcgcgc gcccagcgcc tggcgaagcc cgccggaaaa
     1921 ggcgcgctcg atcatggccg aataaaccgc ctgcatgctt ttcacccatc cgcgcgcgaa
     1981 cagcgcattc acgtcgttcg gattcgtctt gagctcggca ttcgcctcgt cgatcgcctg
     2041 cgtgctcagc tcgccaatgc gatcgtgtac ctttggatct tcgacgaccg tgtgtttgcc
     2101 ggtgagaaat ccatcgttgg catagaacgt ggtgtcgagc aggtcgagcc ggttcagttc
     2161 ccgaaagagc gtcgcgttca gcagataagc ggtcgccatg ggattgccgg gatgttcttc
     2221 gcgaatcttc tcgaagccag gcagagcctc gtcgtaatcc atgttgtaga aatgccggaa
     2281 cgtctcctga acgtcgtgct cgcggttcag cggattggta tccacctgct cggcgcgcgc
     2341 gcgcatcgct ccgaggggcg acagggtcac gcacaacaca gcagaaatcc agccggcacg
     2401 aaaagaggac agcatcgctt cagggattgt agctggcggc gcgcagactc cacccgccgt
     2461 ggtcccgcgc gtctgcgatc ggcagcgcaa tataatccgc tgcatgctcg aatggatcgc
     2521 gctggtcgaa cttcttctct gctggattgt gtgggcgatg gccttcatcc gccccaggcg
     2581 cgaggccgcc gatcgtaaag aggtggcgaa gtccggcgct tcggtgtggg gcatcggcct
     2641 ggtgatggcc gcctatgccc tgacattcac ctatgtccgg ccccgcgggt ttcacaaaac
     2701 agcgccagcg ctcatcgccg gaatgattct ggcgcctccc tgcgtcgttc tggcatggac
     2761 cgcggccagc cacctcggca ggcagtggcg ctacgccgcg gcgttgcgtg aggatcacga
     2821 gctgatccag accgggccct atcgccggct gcgccacccc atctatgcgt cgatgttcgg
     2881 catgctgctg gctaccggat tgtgctggac ctggtggccg caattcgccg tcgctgttgt
     2941 cttctttgtc atcggtacgg agattcgggt tcgatccgaa gagcgcttgc tcgaatcgca
     3001 tttcggcgat cggtaccgtg actaccgtgc gcgcaccgat gcctggattc ccttcctgcg
     3061 ctgatcactc tgcaagagtt cggggaaagg cgttcgttac taaagtacag gggtccatcc
     3121 gaagtactgt gcagcctcct cagcccagat aagagtcacg ttactggcca gcgccacttg
     3181 aaaccaaaat ttgtttcgca actccctgct tccacagcga acctattaac taaagcgtca
     3241 tcataaaaag atagagtgtc tcgtgaccag cgcaatacgg ctgcaccatt ttcgacaacg
     3301 ccacgcgcaa tttcctgttg cctccatgcg gacatctgga ttaccgttgg ggccaccagg
     3361 agcggaacct ctggacgtta ctactaatct ttccgcgaaa gacccatgac tcgccaggaa
     3421 ctcctccgca tcgtcgtgct gcgcgctcgc gtgcatgggt tcgagtttcg tcactggttc
     3481 cagacgaatc tcgagacgga atggcccggg ttcgaccagg ctgtctcgct gctttgctcc
     3541 ggacgccgct actacgctct gctcttttcc cacgatttcg cgcggcactt ctggaagaag
     3601 ggaacgcaga tcaccttcct gctgccttcc caggaataca cgcggctgga ttcgaacggc
     3661 aaagtcgtca ccatcaaacg caagtcgttc acgcgtcgtt cttcgcgctc caactccaat
     3721 aacgtctggc aataccacct cgagcagatg gccgtctggg acgagccgct ccggtacatc
     3781 cgccgtttca ttttgacgga tgaggaactg gaagccgccg gcgagtccgc tccccaggtc
     3841 gtggaagctc cggaaccggc gcccgtcaaa tctgccgaga caaaaatgca ggtagccccc
     3901 cggcgcgcag actggtgctg agccccgcaa ttcctctttt cgccccccaa tccagcctca
     3961 ctgcgacccc gttttcagac ctgctcgccg attgcccggc cccgtcggaa ctgctattct
     4021 cgaaagagtc gcaagacgct tcccgcgcgc ccttagctca gttggataga gcgactgact
     4081 acgaatcagt aggtcgggag ttcgaatctc tcagggcgcg ccacttcctc aacatcctga
     4141 ttttcatcct ccttgctgtg gccgcgacgc gccacggact cacgcaaccg cgggaacctg
     4201 ctccatttcg tcggcgga
//
LOCUS       DASZYN010000024         6120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1289, whole genome shotgun sequence.
ACCESSION   DASZYN010000024 DASZYN010000000
VERSION     DASZYN010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VHE33_00630"
     CDS             <1..262
                     /locus_tag="VHE33_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95978.1"
                     /translation="IYPVQLKTKANQQWAAQRETQRRIRLALEEHGMLPGDPMRVYAR
                     NGDPRSATGVPGATAAKGHEHAAEKTDPTATKPAEGNPLTGE"
     gene            complement(439..1716)
                     /locus_tag="VHE33_00635"
     CDS             complement(439..1716)
                     /locus_tag="VHE33_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HVT95979.1"
                     /translation="MNLFCRVPCRTKLFLIAGLLGLSTSLAGLAQILPLSKHQIIAFW
                     CFAIVGGCVALGALALTAWGMMKTLDRLARRACAIAEGDLTGSPFPVVVNDELGNLSR
                     SINVMQEKLQSMVAAIADESAGLRRDATELSRVGEQAFSRTSEQSAQTHQAASAMQEM
                     SISIAEVSHHAQTAADQGREAASVAREGGTIVDDMLSGMNTISESVSQSAETVRRLGQ
                     DSEQIIRIVNVIEEIAEKTNLLALNAAIEAARAGEQGRGFAVVAGEVRRLAESTRSAT
                     SEIAQMVQTITQNTQAAVQAMASGTERVGHGMEITARAGNSLKRIIAATDQVESMIAQ
                     IATASTQQSVAAQEFSQNLEVINRLGEDQSAATSATRTLVDSVRDGAGRLEESVGHFR
                     IGDKRSAVSVQSSETAGDRCSAFRVLKSTSAIA"
     gene            complement(2023..2592)
                     /locus_tag="VHE33_00640"
     CDS             complement(2023..2592)
                     /locus_tag="VHE33_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HVT95980.1"
                     /translation="MPTEVEVKFRVADPEALTRRLRDAGFRVETERTFERNVLYDTPD
                     RRLRAQTAILRVRQYGERWVVTYKCLPQNHDPGAPHKHREETETRVEDGEALGHIFTQ
                     LGFQPAFTYEKWRTEFADAAGHCVLDETPIGVYAELEGAPDWIDATGSKLGIEPGEFL
                     TLSYGRLFEKWREKTGSSAENLTFEEIPG"
     gene            2682..3752
                     /locus_tag="VHE33_00645"
     CDS             2682..3752
                     /locus_tag="VHE33_00645"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HVT95981.1"
                     /translation="METLRLKVDTANLASEASQSAIARAAEILRGGGTVAFPTETVYG
                     LGANALDVEAVKKIFVAKDRPSWDPLIVHIGEGGMLKHVAAQVTGEAARLVERLTAAF
                     WPGPLTMLLPRSAEIPELVTAGLERVGVRMPAHPVTRALLAAAGVPVAAPSANRFGRT
                     SPTTAEHVAEDLDGRIDAILDAGETTHGVESTVVDVRAEGCVIYRPGVITQEQLAAAC
                     GARVWALEGKEEPESAKAAPAPGMGIRHYAPRAKLVLVEGEGRAQMESLAQALRACEA
                     AGDAVGVMLPDEFRSAVAGGQVQVYRWGRLQDAEELAQRLYAGLRELDAAGVTAIVCP
                     MPRAEGIGVAVRDRLLRAANRG"
     gene            complement(3808..4350)
                     /locus_tag="VHE33_00650"
     CDS             complement(3808..4350)
                     /locus_tag="VHE33_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95982.1"
                     /translation="MPQLPGSTVRTLRPQAVKPPVQRGSNALAPAPTPGPVPVPPPAP
                     APPVNTVDLDVIGPRKLIRPTLPDSARRAGRFRRDQEPVLMVRASAPRTGAIQESSHA
                     EAFYLQKQMQTQTPMVFILDDNEQIEGCIEWYDRNVIKIRGTGSSRSRANGRSDSGAR
                     ILIYKSSIKYLYKAGENSPL"
     gene            complement(4540..5646)
                     /locus_tag="VHE33_00655"
     CDS             complement(4540..5646)
                     /locus_tag="VHE33_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95983.1"
                     /translation="MSFKDFHGNEPTVRHLREALAAGRLPHSLILTGPRGAGKYTLAV
                     MLAQAANCLTPPSNPDGLPDFCGTCSNCVRIAESMDLDARCGEAVTARDELRDVDKRE
                     TRILIQTHPDVLVIPPDPPQLLIKIGQVRILTREIYRVPAEARRAFYIFTCASFMKEA
                     ANALLKVLEEPPAHATIVLLAENPGELLPTIRSRAGIFRLGAIPTPEIEKFLAERRRD
                     LKPAERSLIARLAEGAIGRALGFDLSGYLAARADALVLLRNAMGQTDAGDLFKVTETY
                     RAGAEGQEKTEAMMRAASSILEDLLLLKSETPQLVRNIDLQRELGSMATAVTFDWIEA
                     AARGLRQVESGMRRNLLRSLSLDSFAAQLSVGER"
     gene            complement(5933..>6120)
                     /locus_tag="VHE33_00660"
     CDS             complement(5933..>6120)
                     /locus_tag="VHE33_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HVT95984.1"
                     /translation="AAGEGVPLHGYFLWSLLDNFEWASGYSKRFGITYVDFETQKRVP
                     KLSYDFYKEVIRSNSVV"
BASE COUNT         1102 a         1649 c         2270 g         1099 t
ORIGIN      
        1 gatctatccg gtgcagctga aaacgaaggc aaaccagcag tgggcggcgc agcgggagac
       61 gcagcgccgc atccgcctgg cgctggagga gcatgggatg ctgccgggag acccgatgcg
      121 ggtgtacgca cgaaacggtg atccgcgttc ggcgaccggc gttccgggcg caacggccgc
      181 aaagggacac gagcatgcgg cagaaaagac ggatccgacg gcaacgaagc cggcggaggg
      241 caatccgctg acgggggagt gagaagaaca gagctcagtt gtcagttgtc agcgttcaga
      301 aaagctggcg ttcggcgatc tcccaaaggc gagcgttcag ttgtcatcgt cagcgttcag
      361 aaaagccggc gttcgacgtt ccgcgctcgg cgttcggaac atcctgcggc ccgtccgcag
      421 ttgggcgggt gccatcgctt aggcgatggc ggaagtgctt ttcagaacgc ggaacgccga
      481 acaccgatcg ccggcggttt ctgaactctg aacgctgact gcggaacgct tgtcgccgat
      541 gcggaaatgc cccacggatt cttcgaggcg cccggctcca tcgcggaccg agtcgaccag
      601 agtcctggtg gccgaagtag cggcggactg gtcctcgccc aggcgattga tgacctcaag
      661 gttctggctg aactcctgcg cggcaaccga ctgctgcgtg gaggcagttg cgatctgcgc
      721 gatcatggac tccacctgat cggtggcggc gatgatgcgt ttgagggaat ttccggcgcg
      781 agcggtgatt tccatgccgt ggccgacgcg ttcggttcct gaagccatgg cctggaccgc
      841 cgcctgcgta ttttgggtga tggtctggac catttgagcg atttcgctgg ttgcgctccg
      901 ggtggattcc gcgaggcggc ggacctcgcc ggcaaccacg gcgaatccgc gtccctgttc
      961 gccggcgcgg gcagcttcaa tggcggcgtt gagggccaga aggttggtct tttcggcgat
     1021 ttcctcgatg acgttcacga tacggataat ctgctccgag tcctgaccga ggcggcgaac
     1081 cgtttccgcc gattgcgaga ccgattcgga gatggtgttc atgccggaaa gcatgtcgtc
     1141 gacgattgtg ccgccttcac gggcgactga ggccgcttcg cggccctgat cggcggcggt
     1201 ctgggcgtgg tgggaaacct ccgcgatgga gatggacatc tcctgcatgg cgctggcggc
     1261 ctggtgggtc tgggcgctct gctcactggt gcggctgaac gcctgctctc caacgcggga
     1321 cagttcggtg gcgtcgcggc gcaggccggc ggattcgtcg gcgatggcgg cgaccatgga
     1381 ctgcagcttt tcctgcatga cgttgatcga acgggagaga ttgcccagct catcgttgac
     1441 gacaacagga aacgggctgc cggtaaggtc gccctcggca atggcgcagg cgcggcgggc
     1501 cagcctgtcg agagttttca tcatgcccca ggcggtcagc gccagcgcgc ccagggcaac
     1561 gcagcccccc acgatggcga agcaccaaaa tgcgatgatc tggtgcttgc tgagaggcag
     1621 aatctgagcc agaccggcca ggctggtgga aaggccgagg agtcctgcga tcaggaacag
     1681 cttggtgcgg cacggaacgc ggcagaagag attcatgggc ggcccttcag ttcgagaaga
     1741 agagtggctc ggggatgcga aggacaggtt gcaggcgaag gggcgacagg atcctgcggc
     1801 ctttgaaccg ggtggagggc tgcgaccagc tgcagttcgc gctccagagg gcgctggaag
     1861 gcgcgccaca gtgctgtgcg gcgccggcgg ctgggcgcaa agacggacga gagcaagcga
     1921 aacctccgcg aaggccaaaa aacgggcgaa gagggagggc tgcaccggac ttcgcccgag
     1981 gtgtgccgtt cactggagct atcggcgaaa cggagaaaag aattagcccg gaatttcctc
     2041 gaaggtgagg ttttccgcgg agctgccggt tttctctcgc cacttttcga aaagccggcc
     2101 gtagctgagg gtgagaaatt cccccggttc gatgccgagt ttgctccccg tggcatcgat
     2161 ccagtccggc gcaccctcca gctcggcgta gacgccgatg ggcgtttcgt cgagaacgca
     2221 gtggccggcc gcatcggcga attcggtccg ccacttctcg taagtgaagg cgggctgaaa
     2281 gccaagctgg gtgaagatgt gtcccagcgc ctcaccgtcc tcgacgcgtg tctcggtctc
     2341 ctcgcgatgc ttgtgaggag cgccggggtc atggttttga ggaagacact tataggtcac
     2401 gacccatcgt tcgccgtatt gacgcacgcg aaggatagcg gtttgagcgc ggaggcggcg
     2461 gtcgggagtg tcgtagagga cgttgcgctc gaaggtgcgt tcggtctcca cacggaaacc
     2521 ggcgtcgcgg aggcggcggg tgagggcctc gggatcggcg acgcggaatt tgacctcgac
     2581 ttcggtgggc atacgaccag cgtacagcgt tcaggggtca gcgttcagcg tcgagacgcg
     2641 accagagttt agagtttaga gattggggag acggtgcgcg attggagacg ctgaggctga
     2701 aagttgatac tgcgaatctg gcgagcgagg cctcgcaatc ggcgattgcg cgcgccgcgg
     2761 agatcctgcg cgggggcggg acggtggcgt tcccgaccga aacggtctac gggctggggg
     2821 cgaatgcgct ggatgtcgaa gccgttaaga agattttcgt ggccaaggac cggccgagct
     2881 gggatccgct gattgtgcat attggcgagg gcgggatgct gaagcacgtg gcggcgcagg
     2941 tgaccggaga ggcggcgcgg ctggtggagc ggctcactgc ggcgttctgg ccagggccgc
     3001 tgacgatgtt gctgccgcgg agtgccgaga tcccggagct ggtgacggcc ggactggaac
     3061 gcgtaggggt gcgaatgccg gcgcatccgg tgacgcgggc actgcttgcg gcggcagggg
     3121 ttccggtggc ggcgccaagt gcgaaccggt tcgggcgtac gagtccaaca acggcagagc
     3181 atgtggcgga ggacctcgac gggcgtatcg acgcaatcct ggacgctggg gagacgaccc
     3241 acggtgtcga gtcgaccgtg gtggatgtgc gcgcagaggg ctgtgtgatt tatcgcccgg
     3301 gcgtgattac gcaggagcaa ttggcggccg cttgcggggc gcgggtctgg gcgctggaag
     3361 ggaaagagga acctgaatcg gcaaaggcgg ctccggcgcc cggaatgggg atacggcact
     3421 atgcgccgcg ggcgaagctg gtgctggtcg agggggaggg tcgcgcgcag atggagagtt
     3481 tggctcaggc gttgcgggcg tgcgaggcgg ccggcgacgc ggtgggcgtg atgctgccgg
     3541 atgaatttcg ctcggcggtc gccggagggc aggtacaggt gtaccgctgg ggacgattgc
     3601 aggatgcgga agagctggcg cagcggctct atgccgggtt gagagagctg gatgcggcgg
     3661 gtgtgacggc gattgtgtgt ccgatgccgc gcgcggaggg gattggggtg gctgtgcgcg
     3721 atcggctctt aagggccgcc aacagggggt agccagggac gtgttccgcc ggcgatcgag
     3781 cgctgccttg agagttggtc ggcgcgtcta aagcggcgaa ttctctcccg ctttgtacag
     3841 gtacttgatg ctggatttgt agatcagaat gcgggcgccg gaatcggagc ggccgttggc
     3901 gcgggaacgc gaggagccag taccgcgaat cttgatcacg ttccggtcgt accactcgat
     3961 gcagccttcg atctgctcgt tatcgtcgag gatgaagacc atgggggtct gggtctgcat
     4021 ttgcttctgc aggtagaacg cctcggcatg gctgctttcc tggatagcgc ccgtgcgggg
     4081 ggcggaagcg cgcaccatga gcacgggttc ctggtcgcgg cggaaacggc cggcgcggcg
     4141 cgcggaatcg ggcagagtgg gacggatgag tttgcgcggg ccgatgacgt cgaggtcgac
     4201 ggtattcacc ggaggcgcgg gtgcgggagg gggcactgga accggacccg gcgtcggcgc
     4261 tggagcgagg gcgttcgatc cacgctgtac ggggggctta accgcttgcg ggcgcagagt
     4321 ccgcaccgtc gagccaggca actggggcat tcccataatc ctttacggac aagatgtgcc
     4381 tatgtgatgc agaaaagcgt tgctgcgtcg cacggggaca agaaggcctg aggaaatatt
     4441 ccctggatcg tatcctggat ggcgtgtggg aatccaccgg agggtgcagg atgcagggca
     4501 aagagcgcag gatgacgcgc gagctacagc attgaagagt cagcgttcgc caacgctcag
     4561 ctgcgccgcg aagctgtcga ggctgagcga ccggagcagg ttgcggcgca tgccggattc
     4621 gacctggcgg aggccgcggg cggcggcttc gatccagtcg aaggtgaccg cagtggccat
     4681 ggagccgagt tcgcgctgga gatcgatgtt gcggaccagc tgcggcgttt ccgacttgag
     4741 gagaagaaga tcttcgagga tcgaagaggc ggcccgcatc atggcctcgg ttttttcctg
     4801 tccttcggcg ccggcgcgat aggtttcggt gactttgaag aggtcgccgg catcggtctg
     4861 gcccatcgca ttgcgtaaaa ggacgagggc gtcggcgcgg gcggcgaggt acccggagag
     4921 atcgaagccc agggcgcggc cgatggcgcc ctcggcgaga cgcgcgatga gggaccgctc
     4981 ggcgggtttg aggtcacgtc ggcgctcggc caggaacttc tcgatttccg gcgtagggat
     5041 agcgcccagc cggaagatgc cggcgcggga gcggatggtg gggagcagtt cgcctggatt
     5101 ttcggcgagg aggacaatcg tcgcgtgcgc gggcggttcc tcgaggactt tgagcagggc
     5161 gttcgcggcc tccttcatga aagaggcaca ggtgaagatg tagaaagcgc ggcgagcttc
     5221 ggctgggacg cggtagattt cgcgggtgag gatgcggacc tgaccgatct tgatgagcag
     5281 ttgaggcgga tcgggcggaa tgacgagaac gtcggggtga gtctggatca ggatgcgcgt
     5341 ctcgcgcttg tccacgtcgc ggagttcatc gcgggccgta acggcctcgc cgcaccgggc
     5401 gtcgaggtcc atcgactcgg cgatgcggac gcagttggaa caggtaccgc agaagtccgg
     5461 cagaccgtcg gggttgctgg ggggcgtcag gcagttcgcg gcctgggcga gcatgacggc
     5521 gagcgtgtat ttgccggcgc cgcgcggccc ggtaaggatc agggagtgcg ggaggcggcc
     5581 ggcggcgagg gcttcgcgga ggtggcggac cgtgggttcg ttgccgtgga agtctttgaa
     5641 cgacacaaag acagcgtaca gcgttcagcg ctccgtttta aacctcagct tctcgcgcat
     5701 cgaccagcgc actttgccgg tttccgcagc cagtgtggtg tttctgaagt tcttttccct
     5761 tgtcatcccg atcggactct gagcgtagcg aaggggaagc ggagggacct gcttctcttc
     5821 cgcataaaga actttcggaa acaccacacc aggctcccag acagcttcgg catttgcctt
     5881 tgacgattgc cctgacaact gaacgctgaa cgctgacaaa tgaaagctgt tctcacacca
     5941 cggagttgct gcggatcacc tctttataaa agtcatacga cagcttcggc acacgcttct
     6001 gcgtttcgaa gtcaacatac gtgatgccga accgcttcga atatcccgag gcccattcga
     6061 agttgtcgag caggctccag aggaagtatc cgtggagggg tacgccttcg cctgccgcgc
//
LOCUS       DASZYN010000025         9714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_514631, whole genome shotgun sequence.
ACCESSION   DASZYN010000025 DASZYN010000000
VERSION     DASZYN010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 9714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9714
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514631"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..867)
                     /locus_tag="VHE33_00665"
     CDS             complement(7..867)
                     /locus_tag="VHE33_00665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT95985.1"
                     /translation="MPDMTIRQATQADIAACAAICYNAFAAINAEHNFPCDFPGPEAA
                     LGAITLMFGHPGFYCVVAEVDGRIVGSNCLDERSVIAGIGPITVDPSVQNLRVGRRLM
                     QVVMDRAIERGAAGLRLVQAAFHNRSLALYTSLGFDIREPLTCVQGRTGQRQIPGCAV
                     RAAQPADLPASDALARRIHGFDRSSELADGIQFGSAVVVERGGRITGYASALAFFGHA
                     TAETNLDLQALIASAESFGGPGILVPSRNASLLRWCLSEGLRIVQPMTLMSFGLYNDP
                     AGAWLPSISF"
     gene            complement(1040..1678)
                     /locus_tag="VHE33_00670"
     CDS             complement(1040..1678)
                     /locus_tag="VHE33_00670"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HVT95986.1"
                     /translation="MSLGKAGVHARRQTLGKTQSTAHAKLESHGHDGLRVVTTTQALQ
                     TPVLVLNASYEPINICGARRALVLVLKGVARTEEEQSSELHSARLRMAMPSVIRLLEY
                     RRIPHQTRALSRKNILLRDRNTCQYCSVILPSAELTLDHVVPRSRGGLSTWENLVAAC
                     HSCNRRKGNHLLNELEHMKLQREPRPFTLHTSRHIMRMIGHSDLKWRKYLYY"
     gene            complement(1734..2720)
                     /locus_tag="VHE33_00675"
     CDS             complement(1734..2720)
                     /locus_tag="VHE33_00675"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HVT95987.1"
                     /translation="MNPVHRGIRDPEVPPSDTSAWRAVVRVLRSSARATLDSLTSVIF
                     PADCRVCGQPLADFSLLPICDSCWNDLPGQTGPLCARCGEALDWDPSATGETECRPCR
                     AVPPDFTHAVAHGVYNGTLRAVLHLLKYDGMERAAKRLGMLLADRVLAIPDLPAELLV
                     VPVPLYGKKRRSRGFNQSELLARSLLEALRQRRPEMRVKLAAPLLVRQRATESQAGLT
                     PHQRRENVRGAFFVPRPETVRDRHVMLIDDIYTTGATARACSTALGRAGAATIWVATV
                     ARAQREHAVAPAVEDLQVGVRQDGELKAELPMEQDFVFWEEPGRSHADGEAR"
     gene            2884..4005
                     /gene="queG"
                     /locus_tag="VHE33_00680"
     CDS             2884..4005
                     /gene="queG"
                     /locus_tag="VHE33_00680"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HVT95988.1"
                     /translation="MIDPHRIDELAREAGFTAAGVAAVPEPNSPEDLEERRRFSAWVE
                     TGSAGDMEYLKRRNDAGHLLRSSVRVALPWARSVIVCAANYNSAQPRSINPAPPGSGW
                     IARYAWSTRIDNGTPKPSDYHKVLLRRLEKLRDRLTEQTGPFESRCFVDTGPVVERVY
                     ARYAAIGWVGKNTCILNQKLGSFLFLAVIVTSLEVNEAQRATLPPDRCGSCTRCIDAC
                     PTRALNGDRTMDAARCISYLTIEKRGPIPEDLRPGIGRQIFGCDICQDVCPWNRRAPI
                     AADPELAPRKELVNPALDDLAAMDEAEFERTFNGSPVRRAKWTGFRRNLAIAMGNSGQ
                     TRFLPRLLAWAEDTDPVVADAAAWAVENLRRMEYGGERL"
     gene            complement(4111..4359)
                     /locus_tag="VHE33_00685"
     CDS             complement(4111..4359)
                     /locus_tag="VHE33_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HVT95989.1"
                     /translation="MATTQTQTKKESQVKEHGVVKWFNGAKGYGFIQRSSGEDVFVHF
                     SAIQDSGYRTLNEGEAVEFECQQGPKGLSAANVVRNGA"
     gene            4728..5729
                     /locus_tag="VHE33_00690"
     CDS             4728..5729
                     /locus_tag="VHE33_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HVT95990.1"
                     /translation="MEDGLETQAAGKLDQSPTPAAKEALPPPAPEPPARSQGRGWRAL
                     VLGLARYLAQTEVHTYAFSVAANAILSIFPFIVMMFTVAQNVFHSPAMTREISGILHD
                     VLPSNQDFVVRNMSLLVNPSGGVELASVIMLLISSTGVFLPLEVALNRVWGVERNRSY
                     LMNQLISLGLAGLIGLLTLIAVAAATAQTSILTFLFFGHNQNAAYAFLHQIFLQIAVA
                     FLSIGMFFLIFWILPRRRLPLRAVLPTAVICGLLWDLGRLLYILVLPHMDLHSVYGPF
                     EVSVSLMIWAFLTGLLLLSGAQYSATRHALRLAHEADLEAAKSEQKSDSNSEEAARG"
     gene            complement(5786..6241)
                     /locus_tag="VHE33_00695"
     CDS             complement(5786..6241)
                     /locus_tag="VHE33_00695"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVT95991.1"
                     /translation="MRLAIQIALFLMLTTAVPARSLSQTSASDDEHELMHLEDVWNHA
                     QVSGDADALSHIWADDLELTVPRMPVMTRDEVLNFARSGRMKFLRYTTSDVRVRVYHD
                     AAVVTGRLQRTRSMNGKEISDDWQFTKVYIREAHEWRVVSFHASDTPEP"
     gene            6508..6927
                     /locus_tag="VHE33_00700"
     CDS             6508..6927
                     /locus_tag="VHE33_00700"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVT95992.1"
                     /translation="MHNAGMNDSPESRKAVFRRLVDLYAAGDVSALDQVVAPNYVGHP
                     SAGDRDLEGLRQSILYFHNLFIYTRDSFHIEDQLVDGEKVVTRMTANVQIRETGEPVT
                     LLGINIARIVDGKLVEEWNTWEQTKTTEKLNPSVLSA"
     gene            complement(7128..7796)
                     /locus_tag="VHE33_00705"
     CDS             complement(7128..7796)
                     /locus_tag="VHE33_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95993.1"
                     /translation="MRAVMLRGLTLTAALAAFGACFVLSPCLHAQVVATAPEGEAPQN
                     LQRWDFYGGAQYSHFNPSPGRGVHAINLLGWNGTATVWLGSRVGFDGSVRGLYGTLEI
                     PANNEGIPATAAMNEHLFLFGPNFRMIRHTNYTFGMHTLIGAAYGSFGNEFPKGTKPQ
                     DVGIYNDKLAMGFGIGPWVDYNLGPKLSVRLISDWQPTHYGYSWQSEFAGSVGIVYKI
                     GARK"
     gene            complement(8106..8495)
                     /locus_tag="VHE33_00710"
     CDS             complement(8106..8495)
                     /locus_tag="VHE33_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT95994.1"
                     /translation="MHRFLVVSLLSSLALSASAATPKPARDGSGAAPVSFVVSTGVTE
                     AKLLRTASINIPTNSFDIHANSAELVLCVNLDETGTPTGMHVVHSINPEVDSRVMKAV
                     RSFRWRPAVLDNQTIPVDVNLVVQVQH"
     gene            8630..>9714
                     /locus_tag="VHE33_00715"
     CDS             8630..>9714
                     /locus_tag="VHE33_00715"
                     /inference="COORDINATES: protein motif:HMM:NF016593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HVT95995.1"
                     /translation="MKYDGNFYAPDKPSLLAALRKENAGCKTQPQPRPLRYTLSAGRY
                     TLKLMRPSLPDRAHFLRLARTHSLVPLYRTLTADLETPVSAFLRLAANEPECFLLESV
                     EGGEHLGRYTYIGIRPSRRIVSHGTYIEITEHGKTRVVIGDVFALLRDALQGHKPARI
                     PGLPPFTAGAVGFFTYDVVRRIERLPVHTTDDLQLPDACLMFFDEVLAFDHVKKEILL
                     IVTADLRRHKPQAAYADALRRLDRFEKRLAQPLPKLSAKKPAGKLRPSSRTKKAAFVR
                     AVERAKEYIAAGDIFQVVVSQRLDFEPGVDPFSVYRALRIVNPSPYMYFLRFPRKVAD
                     AKSAKGVRGSKEETTHIVGSSPELLVRV"
BASE COUNT         1905 a         3293 c         2717 g         1799 t
ORIGIN      
        1 atcacgctaa aacgagatcg acggcagcca cgcgcctgca ggatcgttgt agagaccgaa
       61 gctcatcagc gtcatcggct gaacaatgcg cagcccctcg gaaagacacc agcgcagaag
      121 actggcgttg cgcgacggaa caagaatgcc cggtccgcca aacgactccg ccgaagcgat
      181 cagggcctgc aggtcaagat tcgtttccgc tgtggcatga ccgaaaaagg ccagagcgct
      241 ggcatagccc gtgatgcgtc ccccgcgttc caccacgacc gcgcttccaa attgaattcc
      301 atcggccagt tcgctgcttc gatcgaatcc atggatccgc ctggcaagcg catcgcttgc
      361 cggcagatca gccggctgcg cggcccgcac ggcacacccc ggaatctgcc gctggccggt
      421 tcgcccctgc acacacgtca ggggttcgcg gatatcgaag ccgaggctcg tatacagcgc
      481 cagcgatcgg ttatggaaag cagcctgcac aagacgaagg ccggccgcgc cgcgctcgat
      541 ggcccgatcc attaccacct gcatgagcct gcgtcccacg cgcaggtttt gtacggacgg
      601 atcgacggtg atgggaccaa taccggcgat caccgaccgc tcatcgagac aattgctgcc
      661 cacgatgcgc ccgtcgacct ccgcaaccac gcagtaaaag ccggggtggc cgaacattag
      721 agtgatcgca cccaaagccg cttccggccc cgggaaatcg caggggaaat tatgttcagc
      781 attgatggca gcgaaagcgt tgtagcagat ggcggcgcag gcggcgatgt cggcttgcgt
      841 agcctggcga atcgtcatgt ccggcatgct gtttctcttc gaaagcaggc agcagtatac
      901 cggtgtccgc gggccccatc gcgacggttt tcgcggtacg aatcttctgc agacagattt
      961 tggcgaactg gctttccagc gaaccgcacc gcagcttctc agcgaaccgg atttcagcta
     1021 atcgcttctt agctgttcct cagtagtaca aatacttccg ccacttcaga tccgagtggc
     1081 cgatcatccg cataatgtgg cggctggtat gcagcgtaaa cggccgcggt tcccgctgca
     1141 gcttcatgtg ctccagttcg ttcagcaggt gattgccctt gcgccgattg caggagtgac
     1201 acgccgcgac caggttctcc caggtcgaca agcctccgcg cgaccgcggc accacatgat
     1261 ccagcgtcag ttccgccgat ggcagaatca cgctgcaata ctggcaggtg ttccgatcgc
     1321 gcagcaaaat attcttccgc gacagcgccc gcgtctggtg cggaattctt cgatactcga
     1381 gcagccggat caccgacggc atggccatcc gcaagcgcgc ggagtgcagt tcgctgctct
     1441 gctcctcttc ggtccgcgcc acccccttca gcaccagcac cagcgcgcgc cgcgcgccgc
     1501 aaatattgat cggctcatag gaggcgttga gcaccagtac cggcgtttgc agagcctgag
     1561 tcgtcgttac gacacgcaat ccgtcatgac cgtggctttc cagcttggcg tgcgcagtgc
     1621 tctgcgtctt ccccaaagtc tgcctgcgcg cgtgaacacc agcttttccc aacgacatac
     1681 atgcctcctg tcgaaggccc tggtcctggc gatccggctg ccagttcggg cagttatcgt
     1741 gcttcgccgt cagcgtgcga acggcctggt tcctcccaaa aaacaaaatc ctgttccatc
     1801 ggcagctccg ccttcagctc tccgtcctgc cgcactccga cctgcaaatc ctcaaccgcc
     1861 ggcgcaaccg catgctcccg ttgcgcgcga gcgacggtgg caacccaaat cgtcgccgcg
     1921 cccgcgcggc cgagcgccgt cgagcaggcg cgcgcagtcg cgcccgtcgt gtagatgtcg
     1981 tcgatcagca tcacatgccg gtcccgcacc gtctccggcc tggggacaaa aaaagcgccg
     2041 cggacattct cacggcgctg atgcggggtg agccccgcct gactctcggt tgcacgctgc
     2101 ctcaccagca gcggcgccgc aagtttgacg cgcatctcag gacggcgctg ccgcagcgcc
     2161 tcgagcagcg agcgcgccag cagctccgac tggttgaagc cgcggctcct tcgcttcttg
     2221 ccgtacaacg gcaccgggac gacgagcagt tcggccggca gatccgggat cgccagcact
     2281 cggtcggcga gcagcatgcc caggcgcttc gccgccctct ccatgccgtc atatttcaga
     2341 aggtgcagca ccgcgcgcag cgtaccgttg taaacgccgt gcgccaccgc atgggtaaag
     2401 tcgggaggca ccgcacggca gggacggcat tccgtctcgc ccgttgcgga tgggtcccag
     2461 tccagtgctt caccacaccg ggcgcacagc ggtccagttt gtccgggaag atcgttccag
     2521 caggaatcgc agatgggcag gaggctgaaa tcggcaagcg gctggccgca gacgcggcaa
     2581 tcggccggga aaatgacgga ggtgaggctg tcgagggtgg cccgagcgct acttcgaagc
     2641 acgcgaacca cggcacgcca tgcggaggta tcggatggcg gaacttccgg gtcgcggatt
     2701 ccccggtgaa ccgggttcac gggggtgttc gtcagcgaaa cgctctgact gaagacacac
     2761 ctcatttcgg aggagccgcg ggggcccctc gttgggtgca gtatacgagg gttccatcaa
     2821 cccggcaaca actgcctttg ccggtaaggt ggcaaaggta ctctgaaaca gggcgcagga
     2881 atagtgatcg atccgcacag aatcgacgaa ctcgcgcgcg aagccggctt taccgccgcc
     2941 ggcgtcgccg ccgttcccga accgaactcc cccgaggacc tggaggaacg ccgccgcttt
     3001 tccgcctggg ttgaaaccgg cagcgccggc gacatggagt acctgaagcg ccgaaacgat
     3061 gcgggccatc tcctccgctc ctcggtgcgc gtcgcccttc cctgggcgcg gtccgtcatc
     3121 gtctgcgcag ccaactacaa ttccgcccag ccacgctcca tcaaccctgc cccgcccgga
     3181 tccggatgga tcgcccgcta cgcctggtcc acccgcatcg acaacggcac cccaaagccc
     3241 tcggactatc acaaggtcct gctgcgccgc cttgagaaac tccgcgatcg cctcaccgag
     3301 caaaccggcc ccttcgaatc ccgctgcttc gtcgacaccg gtcccgtcgt cgagcgcgtc
     3361 tacgcccgtt atgcagccat cggctgggtt ggcaaaaata cctgcatcct gaatcagaaa
     3421 ctcggctcat tcctgttcct cgccgtcatc gtcacctcgc ttgaagtgaa tgaggctcag
     3481 cgcgcaacgc tccccccgga ccgctgcgga tcctgcaccc gctgcatcga cgcatgcccc
     3541 acccgcgcgc tcaacggcga ccgcaccatg gacgcggccc gctgcatctc ctacctgaca
     3601 atcgaaaagc gcggccccat ccccgaagac ctccgccccg gcatcggccg ccaaatcttc
     3661 ggctgcgaca tctgccagga cgtctgcccc tggaaccgcc gcgcccccat cgccgccgac
     3721 cctgaactgg ctccgcgcaa agaactggtc aaccccgcgc tcgacgacct cgccgccatg
     3781 gacgaggccg agttcgagcg caccttcaat ggctcgcccg tccgccgcgc gaaatggaca
     3841 ggctttcgcc gcaacctcgc catcgccatg ggcaacagcg gacagacgcg cttcctcccg
     3901 cgcctgctgg cgtgggccga agacaccgat ccggtcgtag ccgatgcggc cgcgtgggcg
     3961 gtggaaaacc tgcgacggat ggagtacggc ggggaacgac tctgatcgtc tgattgtgac
     4021 ccggccaggg ccagcctcgg cccctggaga aattccggaa ggatctgcga ggcttccgtg
     4081 ggggtcgaag cctcgcagcc ctgagatcgc ttaggcgccg ttgcgtacta cgttcgcagc
     4141 gctcagcccc ttgggaccct gctggcactc gaactcgacg gcttcgcctt cgttcagcgt
     4201 tctgtaaccc gaatcctgga tcgcggagaa atggacaaat acgtcttcgc cgctggaccg
     4261 ctggataaac ccataaccct tcgctccgtt aaaccacttc acgactccat gctctttcac
     4321 ctgggactcc ttcttcgttt gcgtctgcgt tgttgccaac gctgccgtcc ggcttccctg
     4381 catccgttac tggtgtaacg aatggcagag acaccaaaag ctgcctttgc ccgcccagca
     4441 tcggcggttc gggcagctca gggatagacc gggcagtcgc ggtgacagcg atacggatac
     4501 atcagaaact ggccttctcg aatccgattt ccttcctggg gatggaaaat cgtgtacctg
     4561 ttatggcagg aaacgatttt gaaatcaaga aaaatgtgta ccccaaaagt acgttttccc
     4621 gcgggttacc gaagtacaag ccccttttga tcgactgacc ctcgccggaa cccacagaaa
     4681 ctccgccgcc ggtcagcccc tgttacgatt ttttcgagag tatccggatg gaggatgggc
     4741 tggaaacgca ggcggccggc aagctcgatc aatccccgac gcccgccgca aaagaggctt
     4801 tgcctccgcc cgcgcctgaa ccgcccgcgc gaagtcaggg gcgcggctgg cgagcccttg
     4861 tcctcggcct tgcccgctat ctcgcgcaaa ccgaagtaca tacctacgcc ttcagcgtcg
     4921 ccgccaacgc catcctctcc atctttccct tcatcgtcat gatgttcacc gtggctcaga
     4981 atgtcttcca ttccccggcc atgacccgtg agatcagcgg catcctccac gatgtcctgc
     5041 cctcgaacca ggacttcgtc gtccgcaaca tgagcctcct cgtcaatccc agcggcggcg
     5101 tcgaactcgc ctccgtcatc atgctgctca tctcctccac cggcgtcttt ctgccgctcg
     5161 aagtcgccct gaaccgggtc tggggtgtcg aacgcaaccg ttcctacctc atgaaccagc
     5221 tcatctccct cggactcgcc ggcctcatcg ggctgctcac cctgatcgcc gtcgcagccg
     5281 ccaccgccca gaccagcatc ctcaccttcc tcttcttcgg tcacaaccag aacgccgctt
     5341 acgctttcct ccatcagatc tttctgcaga ttgctgtcgc cttcctcagc atcggcatgt
     5401 tcttcctcat cttctggatc ctgccccgcc gccgccttcc cctccgcgcc gttctgccca
     5461 ccgccgtcat ctgcggcttg ctgtgggacc tgggccgcct cctctacatc ctcgtcctgc
     5521 cccacatgga cctgcactcg gtctacggtc cgttcgaggt ttccgtcagc ctcatgatct
     5581 gggccttcct caccggcctc ctcctcctct ccggcgccca gtactcggcc acgcgccacg
     5641 cgttacgcct ggcccacgaa gcggatctgg aagcagcgaa gtccgagcag aaatcagatt
     5701 cgaattccga ggaagccgcg cgcggttgac gccgatcgaa ttcccggccg cgaaataccc
     5761 ggcaacggac gtctcggctt gaggactacg gctctggagt atccgaggcg tgaaacgaga
     5821 cgacgcgcca ttcgtgtgcc tcacggatgt agaccttggt gaactgccag tcgtcggaga
     5881 tttctttgcc attcatgctg cgagtgcgct gaaggcggcc ggtcaccacc gccgcgtcgt
     5941 ggtaaacgcg aacgcgcaca tcggaggtgg tataacgcag gaacttcatt cgccccgagc
     6001 gtgcaaaatt gagcacctca tctcgtgtca tgaccggcat gcgtgggaca gttaattcaa
     6061 gatcatcggc ccaaatgtga ctgagagcgt ctgcatcgcc gctgacctga gcgtgattcc
     6121 acacgtcttc cagatgcatc agttcgtgtt cgtcatcgct ggcggaagtt tgggaaaggc
     6181 tccgtgcagg aaccgcggtc gtcagcatga ggaacagggc aatctgaatc gccagtctca
     6241 tcgtcgatgc tccgggaatc tcgggagatt agccgatctg aaacgccgct ggcaaacgct
     6301 ctgcccgcat ggccggcatc tcgaacattt cgacgccaaa cttctacaca tccttctaca
     6361 attgcgtgac cgcgaccaaa catgaagtgg aaaaagccgc cgagaagatt cgctatggcc
     6421 tcgcgcccgc taagagtagt tcaaccacca gggttggcgc accatggtcg tgagaggcga
     6481 cactttgtaa tgtgagagga aatcgccatg cacaacgcag gtatgaacga ttctccggag
     6541 tccaggaaag cggtctttcg aagactagtg gatctatatg ccgctggaga cgtttcggcg
     6601 ctggatcaag tcgttgcacc caattatgtc gggcacccgt cagcgggcga ccgcgatctg
     6661 gaggggctcc gccagagcat cctgtacttc cacaacctct tcatctacac cagggattcc
     6721 tttcacattg aggatcaact ggtcgacggc gagaaggtcg tcactcgtat gaccgccaat
     6781 gtccagatac gagagaccgg cgaacccgtt acgctgctgg gtatcaacat cgctcgcatt
     6841 gtggacggaa aacttgtgga agaatggaac acctgggagc aaacgaaaac aactgaaaag
     6901 ctgaacccat ccgtactgag tgcctaagcg actctgcagc accgctcgcc tcgcgatgct
     6961 gtgcaacacc catcaggaac cgttgtcgct tcagctcaga accggtggca tttcctcgca
     7021 agcgaggaaa tgccagggca agagggcggg cggccggggc cccggcgaaa taacgtttcg
     7081 ctggggtggc ctgaaggtgg accatttggg ggattgttgc cgagcagtta cttccgcgcg
     7141 ccgatcttgt acacgatccc caccgacccc gcgaactcac tctgccagct gtatccatag
     7201 tgcgtcggct gccagtccga gatcagccgc accgacagct tcggccccag gttgtagtcg
     7261 acccacggcc cgatcccgaa ccccatcgcc agcttgtcgt tgtagatgcc cacgtcctgc
     7321 ggcttggtcc ctttggggaa ttcattgccg aacgacccat acgccgcccc gatcagcgtg
     7381 tgcatgccaa aggtgtagtt cgtatggcgg atcatccgga agttcggccc gaacaggaac
     7441 aggtgctcgt tcatcgccgc cgtggcggga attccctcgt tgttggcggg aatctcgagc
     7501 gtgccgtaca gtccgcgcac cgatccgtcg aagccgacgc gcgaaccgag ccacaccgtc
     7561 gccgtgccgt tccagcccag caggttgatc gcgtgcacac cccgccccgg gctgggattg
     7621 aagtgcgagt actgcgcgcc cccgtagaag tcccagcgct gcagattctg cggcgcttct
     7681 ccctcaggag ccgtggcaac cacctgcgcg tgaaggcaag gcgaaagaac gaaacaagcc
     7741 ccgaaagcgg ccagggcggc agtcagggtc aggccacgca acatcacggc tctcacgcga
     7801 tataaaacct caaaccaaaa gatcgagcac acggctcgga taccaccaaa cgcatagcag
     7861 gaattggacg gcaagaaaca agggtatcac ctggagcgcg cccctcggga accgctccgg
     7921 cgcccgcttc ttcgaccgcg cgcgaccgcg tttcgtctgc aaaaaagatt cctccagtcc
     7981 ggcggatcaa tatgccggac cagaggacca tggcgctgcg cagcaggggc cggaagacac
     8041 cacacccgga gcgagcgagg cgctcactga agaattttgc tggttgttgt gacttgttcc
     8101 ggggcttagt gctgaacctg gaccaccaga ttcacatcga ccgggatggt ctggttgtcg
     8161 agcaccgccg ggcgccagcg gaagctgcgg acggccttca tcacgcgcga atccacttcg
     8221 gggttgatgg agtgaaccac gtgcataccc gtaggtgtcc cggtttcatc gaggttcacg
     8281 caaagtacga gttcagccga gttagcgtgg atatcgaacg agttagttgg gatgttgatg
     8341 ctggcggtgc ggaggagttt ggcttcggtg acgccggtgg agaccacgaa cgataccgga
     8401 gcagctccgg aaccgtcgcg agcgggtttg ggggtggcgg cagacgcgct gagcgcaaga
     8461 gatgagagca gactaacaac gaggaaacgg tgcatgggga acctccgttg ggtcgatagt
     8521 gaccgtcttc actttaatcc gactaagata cgattgtcaa gattaatttg taagtgctgg
     8581 ataggtggtc agttagtcgc tgagttaggt cggaaattaa caaaaggcta tgaaatatga
     8641 cggaaacttt tatgcgcccg ataaaccatc tctgctggct gctctccgca aagagaacgc
     8701 cggctgcaag acccaacctc agcctcgccc gctgcgctat acgctctccg ctggacgcta
     8761 tacgctaaaa ctcatgcgcc cctcgctgcc cgaccgcgcc cactttctcc gcctcgcgcg
     8821 cacgcactcc ctcgtcccgc tctaccgcac cctcaccgcc gacctcgaaa cgcccgtctc
     8881 cgccttcctt cgcctcgcag ccaacgagcc cgagtgtttc ctcctcgaat cggttgaagg
     8941 cggcgagcac ctcggccgct atacctacat cggtatccgt cccagccgcc gcatcgtctc
     9001 gcacggcacc tatatcgaaa tcaccgagca cggcaaaacg cgggtcgtca tcggcgacgt
     9061 cttcgcgctc cttcgcgatg ccctgcaggg acacaagccc gcccgcatcc ccggccttcc
     9121 gcccttcacc gccggcgcgg tcggcttctt cacctacgac gtcgtccgcc gcatcgagcg
     9181 cctgcccgtc cataccaccg acgacctcca gctccccgac gcctgcctta tgttcttcga
     9241 cgaggtcctc gccttcgacc acgtcaagaa ggagattctg ctcatcgtca ccgccgatct
     9301 ccgccgccac aaaccccagg ccgcatacgc ggacgcgctc cgccgcctcg accgattcga
     9361 gaagcgcctc gcccagccgc tgccgaagct cagcgcgaag aaacccgcgg gcaaacttcg
     9421 cccctcttcc cgcacaaaga aggccgcctt tgtccgtgcc gtcgagcgcg ccaaggaata
     9481 catcgccgcc ggcgacatct ttcaggtcgt cgtctcccag cgcctcgact tcgagcccgg
     9541 cgtcgatccc ttctccgtct accgcgctct ccgcatcgtc aacccctcgc cctacatgta
     9601 cttcctccgc ttcccgcgca aagtcgcaga tgcaaaatcg gcaaaaggcg tccgcggctc
     9661 gaaagaagaa accacgcaca tcgtcggctc ctcgcctgag ctgctcgtgc gcgt
//
LOCUS       DASZYN010000026         3881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_129946, whole genome shotgun sequence.
ACCESSION   DASZYN010000026 DASZYN010000000
VERSION     DASZYN010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129946"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..3728)
                     /locus_tag="VHE33_00720"
     CDS             complement(162..3728)
                     /locus_tag="VHE33_00720"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVT95996.1"
                     /translation="MISRRKPLLFLLLAICLLLWGFSPRASAQNVYAAIHGTVTDASG
                     AVIPHANVTVLNTGTGISTKTTTDDKGYFIFPQLQVGGPYTVTVSAAQFQKFSSTGLT
                     LNVNDNREVDASLSVGAAAQTINVAASAVQVDTSDTQLKQIVTSDQLEQIPLEGRDPA
                     GLGKLNPGVVESSDRFGSYSTNGSQTPQNSYLLSGIDINDGPLQDEGIRVNPDALAQE
                     NIVTSTLNPEFSRNSGQVVNEVIKSGSNSYHGSGFEYYRDTFLNNGNYFSLPGQRPQF
                     HQNLYGGTLGGPVKKDKLFFFLGYQGFRNRTGATTNAQTMSDAQFAGNFTGDTLLSPG
                     AAGGLSDNPIPFAIGACAAGTTWADCFPGNNVNIPTTQWNSIASKVVTQFVPHANNSI
                     GGVPYYNFNAGSTRAQDQGVIRIDYTPTSRDTFWASSVFESHPGTDVLAFGGGDFPGF
                     GSVQSEHFKLFSTSWTHTFNNSMLNELRGGYYRFNFPTVEPSQIVQPSSYGFNITPNI
                     SQAGLPYMSIGANFNLGFSFEGPQPRTDTNLTIADNFSWVLGSHSLKFGASFEQFRVD
                     NPFAYLNNGYYYYDGGSIYSSGDPLIDFVMGVPDGYEQTSDGFINAVATERYAYAQDN
                     WKVSPTLTVNYGVAWDAEQPNQNRQFGGLGIICYVGGSATSNVFPGGPPGLQYPGDPG
                     CNAAGGPTTHYDHFGPRVGFAWSPSSGPEKLIGANGSHDFSVRGAFGVYFNRDQEEQS
                     LQNLTDPPFLYTSQGAADVGGSPAFADPFSDVTGDPSVSEANRFPYTPPKAGGTVDWT
                     IYNYLGMSAFDRKYNTPYVYNFNLNIQRSLPSNMVAQIGYVGSLGRRLATWYEGDPIT
                     PAGHDACASGSVSVFGIPCNDTRVSSKIHEYFPQLTANPAVVPGTVGTVLPNGLPWYS
                     GIGMQNSEGSSNYNSLQASLTKAITHGFQFTLAYTYSHALDNASGYESATGSAPGGGS
                     TGRSMIYTPGYTYLNYGDSDYDARHRLVASYIYVVPVGALGHRNAFFREALSGWEIAG
                     VTAMQTGFPVGIATGQDRSLWCDGGSFFGCGDNPDTSNFHPKSLDPRAAGNAWFDSGD
                     FSLEPLGTFGNTKRNYFHGPGFNYTNLQVSKNFPVSADGSRYVQLRLEGFNAFNHANF
                     SGPDGTFTSPGFGQITSVIQSSDSNADPQPGRAVQLAGKFYF"
BASE COUNT          771 a         1038 c         1299 g          773 t
ORIGIN      
        1 cactcagtgt agccgaaaca ggtgtgcggg gggaaagggg aaagagggga aaacgggcag
       61 gaagcgagtg aaactgcgac ttgcaaaagt aaaggccgcc gcagcggcct ctggaagcga
      121 gtcggcgaag gcccggagac cctcgccggt cttgtcactg gctagaagta gaatttgccg
      181 gcgagctgaa cggctcgtcc gggctgagga tcggcattcg aatcggagga ctggatcacg
      241 ctcgtgatct ggccgaatcc aggactcgtg aacgtgccat ccggacccga aaagttggcg
      301 tggttgaagg cattgaagcc ctccagccgc agctgaacat agcgggatcc gtcagcactg
      361 acggggaagt tcttcgatac ctggaggttg gtgtagttga atccgggccc gtggaagtag
      421 ttgcgtttgg tattcccgaa ggtccccagc ggctccaggc tgaagtctcc gctgtcgaac
      481 caggcgttgc ccgcagcccg tggatcgagc gacttcgggt ggaagttcga tgtatccgga
      541 ttgtctccgc agccgaagaa gctgcctcca tcgcaccaga gcgaacgatc ctgaccggta
      601 gcaatgccga ccgggaagcc ggtctgcatc gccgtgacgc ctgcgatctc ccagcccgag
      661 agggcttcgc ggaagaacgc attgcgatgt cccagcgctc ccaccggaac gacgtaaatg
      721 tacgaggcaa ccaggcgatg ccgcgcgtcg tagtcagagt caccgtagtt gaggtaggtg
      781 tatccgggcg tgtaaatcat ggaacgtccg gtgctcccac cgccaggtgc agacccggtc
      841 gcgctttcgt agccggatgc gttgtccaat gcgtgcgaat aagtgtaggc aagcgtgaac
      901 tggaacccgt gcgtgatggc cttagtcagg ctggcctgca gcgagttgta gttcgaggag
      961 ccttcactgt tctgcatgcc gatgccggaa taccagggca gaccgttcgg cagaacagtc
     1021 cctacggttc ccggtacgac agcggggttg gcggtcagtt gcgggaagta ttcgtggatc
     1081 ttcgaagaga ctcttgtatc gttgcaggga attccgaaga ccgaaacgct cccgctcgcg
     1141 caggcatcat ggccggccgg agtgatcggg tcgccttcat accaggtcgc cagacggcgg
     1201 ccgagcgaac cgacgtatcc gatctgcgcc accatgttgc tgggcagcga acgctggatg
     1261 ttcaggttga agttgtaaac gtagggcgtg ttgtacttcc tgtcgaacgc cgacatgccc
     1321 aggtagttat agatggtcca gtccaccgtt ccacccgcct tcggcggcgt gtacgggaac
     1381 cggttagcct cggacacgga agggtcgccg gtaacgtcgc tgaaaggatc ggcaaaggcc
     1441 ggcgagccgc ccacgtctgc agcaccctgc gacgtgtaga ggaacggcgg atcggtcagg
     1501 ttctgcagag attgctcttc ctggtcgcga ttgaaataga ccccaaacgc tccacggaca
     1561 gaaaagtcgt gggagccgtt cgctccgatc agcttctcag gaccgctcga cggcgaccac
     1621 gcgaagccaa cgcgcgggcc aaagtggtcg tagtgggtgg tgggtccccc ggctgcgttg
     1681 cagcctggat cgccaggata ctgaagtccc ggaggtccgc cagggaaaac attcgaggtc
     1741 gcagagccgc cgacgtagca gataatcccc agcccgccga actgacggtt ctggttcggc
     1801 tgctcagcat cccacgcaac gccgtagttg acggtcagcg tggggctcac cttccagttg
     1861 tcctgcgcgt aggcgtaacg ctcggtggcc acggcgttga taaaaccatc gctggtttgc
     1921 tcatagccgt cgggaacgcc cataacaaag tcaatgagtg gatcgccgga gctgtagatg
     1981 ctgccgccgt cgtagtagta gtagccgttg ttgaggtagg cgaagggatt gtcgacgcgg
     2041 aactgttcaa acgacgctcc gaacttgagc gagtggcttc ccagaaccca gctgaagtta
     2101 tctgcgatcg tcaggttggt atcggtgcgg ggctgagggc cctcaaagct gaagcccaga
     2161 ttgaagttgg cgccgatgct catgtagggc agcccggcct ggctgatgtt cggggtgatg
     2221 ttgaacccgt acgagctggg ctgcacaatc tgcgagggct ccacagtggg gaagttgaag
     2281 cggtagtatc cgccgcgcag ttcgttcagc atgctgttgt tgaaggtgtg tgtccacgag
     2341 gtgctgaaca acttgaagtg ttccgactgt accgagccga atccggggaa gtcgcccccg
     2401 ccgaacgcga gaacatcggt gccgggatgg gattcgaaga cactggaggc ccagaacgtg
     2461 tcccgtgagg tcggagtgta gtcaatacgg atcacaccct gatcctgcgc ccgcgtggat
     2521 cccgcgttga agttgtagta gggaactcca ccgatgctgt tgttggcatg gggcacgaac
     2581 tgggtgacca ctttggaggc gatcgagttc cactgagtgg taggaatgtt cacgttgttg
     2641 cccgggaagc agtctgccca cgtcgtgccg gcggcgcagg cgccgatggc aaaggggatc
     2701 gggttgtccg acaggccgcc tgcggcgcct ggagagagca gggtgtcgcc cgtgaagttg
     2761 cccgcaaact gagcgtcgct catggtctgc gcgttcgtcg tggcgccggt acggttgcgg
     2821 aagccctggt agccgagaaa gaagaacagc ttgtctttct ttaccggccc gcccagggtg
     2881 ccaccataga ggttctgatg gaactgcggc cgctgtccgg ggagtgagaa gtagttgccg
     2941 ttgttcagga aggtgtcgcg atagtattca aagccgctgc cgtggtatga attgctgccg
     3001 gacttgatga cttcgttgac cacctgtccg gagttgcggg aaaactcagg gttcagcgtg
     3061 ctggtgacga tgttctcctg cgccagggca tcggggttga cgcggatgcc ttcgtcctgc
     3121 agcgggccat cgttgatgtc gattccgctc agcaaatagg agttctgcgg cgtttggctg
     3181 ccgttggtcg agtagctgcc gaagcggtca gaggattcca ctacgccggg gttcagcttg
     3241 cccaggcccg ccggatcccg gccttcgagg ggaatctgct cgagctgatc ggaggtgacg
     3301 atctgcttta gttgggtgtc ggaagtgtcg acctgcaccg cgctggcagc tacgttgatc
     3361 gtctgcgccg ccgcgcccac gctgagggaa gcatcgactt cgcgattgtc attcacgttc
     3421 agcgtgaggc cggtcgagga gaatttctgg aactgcgctg ctgaaaccgt aaccgtatac
     3481 gggccaccca cctgcaactg gggaaagatg aagtaccctt tgtcgtctgt cgtcgttttg
     3541 gtggagatgc cggttccagt gttgagcacc gtgacgttcg cgtgcggaat gaccgcgccg
     3601 gaggcgtcgg ttacggtgcc atggatagcc gcatagacgt tttgagcact ggcgcgaggc
     3661 gagaatcccc acagcaagag gcagatggca agcagcaaga acaacagggg tttgcgtcgg
     3721 gaaatcataa tttcctccga aaaagaaatt ggagtgaggt ttgtcgtggg tgttgggaaa
     3781 cgtgcggccc gctgggatcg agtctcgaca ggggcttgaa cggcttctct ttgcgaagcg
     3841 cgaacattga gggattggcc cggtgacggg gccggaacga a
//
LOCUS       DASZYN010000027         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1410, whole genome shotgun sequence.
ACCESSION   DASZYN010000027 DASZYN010000000
VERSION     DASZYN010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1423
                     /locus_tag="VHE33_00725"
     CDS             278..1423
                     /locus_tag="VHE33_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HVT95997.1"
                     /translation="MPHQNIVMHDLVKQVPWARFDELVAAHGTDRGARKLTTKQHLLA
                     MLFAQLLGASSLREIVEGMASHEARLYHLGGAPVKRSTLSEANRGRSAAVFGELLSEL
                     MKQAHRGLRRAMKESVYLIDATVLPLNGLSADWAHYSAGVCGAKAHVIYDPAADCPIY
                     ASVTNRAVNDITAAKAMPIVAGATYVFDLGYYDFGWWAELDAKGCRIVTRFKSHTPLA
                     VTQTRAVPASGPILSDRIGYLPQRQAKNRKNPLQEPVREICLVIETGKILRILSNDLE
                     ASAQEIADLYKRRWAIELFFRWIKQTLEIKKFLGTNENAIRIQIAVALIAFLLLRLAH
                     AALRITFSLLTFTRLVRANLMHRRRIDQLLLRPPSAHLQNQMQLLWT"
BASE COUNT          299 a          499 c          498 g          289 t
ORIGIN      
        1 cggatcatcg ccggcggcat catcgtggtc gcgagctgca tctacatcaa cagccgccgc
       61 gcgaagaaga agcgcgaagc ggcggcgcag gcgtagcgat cacgagaaaa ccgtcattgc
      121 cgggcttgac ccggcaatct cgggcccacc ggaaccagat gcccgggtca agcgcagggc
      181 tgtccggtac aagttttgct ggactgggtg cacggcattg attcaactcg agttcaagcg
      241 tttggcgacg ttttggacaa gagaaggatg caacgccgtg ccgcaccaga atatcgtgat
      301 gcatgatctt gtgaagcagg ttccgtgggc tcggttcgat gagcttgtcg cggcacatgg
      361 gacggatcgt ggcgccagga agctcacgac taagcagcat ttgctggcga tgctgtttgc
      421 gcagctcttg ggtgcttcga gcttgcggga gatcgtggag gggatggcga gccacgaggc
      481 gcggctttac catctgggtg gcgcgccggt gaagcgttcg accttgtcgg aggccaatcg
      541 cgggcgttcg gcggcggtgt tcggggagct gctgtctgag ctgatgaagc aggcgcaccg
      601 cgggttgcgc cgggccatga aggagagcgt ctatctgatc gacgcgaccg ttctgccgct
      661 caacgggttg agcgcggatt gggcgcatta ttcggccggc gtgtgcgggg ccaaggcgca
      721 tgtgatctac gatcccgctg ccgactgccc gatctatgcc agcgtcacca accgcgcggt
      781 caacgacatc accgccgcca aggccatgcc gatcgtggcc ggtgccacct atgtctttga
      841 tcttggctac tacgacttcg gctggtgggc tgagctcgat gccaagggct gccgtatcgt
      901 tacccgcttc aaatcgcata ccccgctcgc cgtgacccag acccgggcgg tgccggcgag
      961 cggcccgatc ctctccgacc ggatcggcta tctgccccag cggcaggcca agaaccgcaa
     1021 gaacccgctc caggagccgg tccgcgagat ttgcctcgtg atcgagaccg gcaagatcct
     1081 gcgcatcctc agcaacgacc tcgaggccag cgcccaggag atcgccgatc tctacaagcg
     1141 ccgctgggcg atcgagctgt tcttccgctg gatcaagcag acgttggaga tcaagaagtt
     1201 cctcggcacc aacgagaatg cgatccggat ccaaatcgcc gtcgcgctga tcgccttcct
     1261 gctgctcagg ctggctcacg ccgcactccg catcaccttc agcctgctca ccttcacgcg
     1321 cctggtcaga gcaaacctga tgcatcgcag gcgcatcgac cagctgctcc tccggccacc
     1381 ttccgcccac ctgcaaaatc agatgcaatt gctatggacc tagccatgca atcaccgtac
     1441 cggacagccc tgggtcaagc ccgggcatga cgattctggt tacccctaat acgtcgcgcg
     1501 acccccggag aggtcgaagg tgaaaccggt ggtgaagctg caggccggcg agacgatcca
     1561 ggcgatcagc ttcgccgcct cgtcg
//
LOCUS       DASZYN010000028        31677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_130021, whole genome shotgun sequence.
ACCESSION   DASZYN010000028 DASZYN010000000
VERSION     DASZYN010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 31677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31677
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130021"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1635
                     /gene="garD"
                     /locus_tag="VHE33_00730"
     CDS             94..1635
                     /gene="garD"
                     /locus_tag="VHE33_00730"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR03248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HVT95998.1"
                     /translation="MKIPPLFVRAHPKDNVAIVVNEGGLPAGSRFESGLVLAEAVPEA
                     NKIALTDLAEGAAIIRYGEIIGHAQRALPAGRWVRDDLVRMPAPPSLNSLPIATAVPK
                     PLPPLEGCTFDGYVNEDGSVGTRNILGITTTVQCVAATVDYAVRRIKSDLLPRYPNVD
                     DVIALTHGYGCGIAIDAPGAEIPIRTLRNLSLNPNLGGEPMVVSLGCEKLQPVTLMPS
                     NTLPVLENRPYIVRLQDEEHRSFGDMVTAIMRMAEERLTRLNARRRTRRPASDLVVGL
                     QCGGSDAFSGVTANPALGYAADLLVRAGATVMFSEVTEVRDAIHLLTARATDEAVAQD
                     LIREMAWYDDYLRRGATDRSANPSPGNKKGGISNIVEKALGSVAKAGSTALVGVVGPG
                     ERVTRKGLVFAATPASDFVCGTLQLAASMNMHVFTTGRGTPYGLAMAPVIKVATRTPL
                     AERWADLIDVDAGTIATGQASIREVGKQIFGMILDVASGRKQTCADRWGLHNDLVLFN
                     PAPIT"
     gene            complement(1658..2497)
                     /locus_tag="VHE33_00735"
     CDS             complement(1658..2497)
                     /locus_tag="VHE33_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HVT95999.1"
                     /translation="MKMTPRDTSSLRDPYRLQCLDRALSVLEYISESDVPLSLADICR
                     SMGLHKSTAHRALMVLERGMLLERTPEDRYRLGLKLYTLGSRAVEQMDLRRQIQPSLR
                     SLSLRLGETVHLGVLQRTSVVYLDKAGPATRVCKGSRTGSSNPAYCTAMGKAMLAWLP
                     QTEVRQLIDQMTFEPLTPKTIGSGGELLQALQRVRRRGYSIDDEEVELGARCIGAPIF
                     DHDHNPIAALSVSCASSRIQPHQVPALAEHLRRTCAEISASLRVHRDLRARVVGSFAP
                     EKN"
     gene            complement(2494..3588)
                     /locus_tag="VHE33_00740"
     CDS             complement(2494..3588)
                     /locus_tag="VHE33_00740"
                     /inference="COORDINATES: protein motif:HMM:NF017263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HVT96000.1"
                     /translation="MRSKCRSGLSITMRAIAAITIAFAAASASTSPLRSPWDDHPIVP
                     TDEPYTCPPIAYLSRDLTTDGFYSDSKASVIDPAKWKAYSESSGPYKNLGQVIVDAAD
                     AWRTSGSRAAATCALSHMEAAAREGVLTGKMSSRQAYYVQGWVIGAIAVSWLKVRDSG
                     LETPAVRDLVISWIVSVARQTVAFYDSSDAKNNHLYWAGVEAAAAGVAANNPRLFGWA
                     VAAYRTGVAQIQPDGSLPLEMARGQRALHYHLYALSPLVYIAEFGQVNGLDLYAERDH
                     ALARLERLCVDGLKNNHFFEKATGIAQDTPTRGAPAAEQISWGVIWQSRFPDAALASI
                     LAQSHSLSYMYLGGLPPGVNRNLAALRENR"
     gene            complement(3578..3919)
                     /locus_tag="VHE33_00745"
     CDS             complement(3578..3919)
                     /locus_tag="VHE33_00745"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVT96001.1"
                     /translation="MSEQFKGLTSLKNVNATHPEDGLIRQVLASNPGMMLVRHQMRPG
                     WRGARHSHPHEQMVYVIRGHIRFICGSESFEAFAGDSFIVPGGMEHEASAVAESEVLD
                     FFVPSREDYAL"
     gene            complement(4147..5298)
                     /locus_tag="VHE33_00750"
     CDS             complement(4147..5298)
                     /locus_tag="VHE33_00750"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HVT96002.1"
                     /translation="MKKILSLLLLGFAMSQGLPTWGQSTPSPANAERAAAGDEPDNPG
                     PLATNLSPALKRTAILKAMRLVANWQLQHAEGRYNIGWTYAALYDGMLAASRTTGDPR
                     YRDRVFEIAKENHWELGPRFGHADDEHIGLTYLTFYAEDHKPDHLAPTKENLDKLLDR
                     PDDPQKNLWWWCDALYMAPPVLAQLSVATGDHRYLDFMDHEWSLTTKALYDPQDHLYY
                     RDDRFLTMHEPNGQKIFWSRGNGWVLAGLALVLERMPQDYPQREKYIALYRDMAARIA
                     TLQPADGLWRSGLLDPEAYKSPEISGSAFFTYAMAWGIHHHLLDKKTYMPVVSKAWRG
                     MLTHIYQDGRLGSIQPIGAAPDQFKPSSSYVYGVGAFLLAGSELAQMTK"
     gene            5509..6864
                     /locus_tag="VHE33_00755"
     CDS             5509..6864
                     /locus_tag="VHE33_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HVT96003.1"
                     /translation="MKNPEITRRSLLKGTGASLLAMGAGVPAGAPMLAAQETSANSTP
                     LPGDTDREERMKWWKAARFGMFIHFGVYSTIGRHEWVMEDEGIPVVEYAPHATTFHPA
                     PGSQRAWARLAKQAGMKYMVMTSKHHEGFCNFNTKLTSYCAPKQGPGRDLIGEYVEAA
                     RAEGLRVGFYYSLMDWHHPDGASCATNEDARKRFVDYTHGLIHELMSNYGKIDVLWYD
                     VAWPLDAQGWESVRMNEMVFRLQPEIIVNNRNKLPGDFSTPEQHITAEKGGRAWEACM
                     TLNDSWGYQAADDNWKSSKTVIRNLISCVRDSGNYLLNVGPKPDGSVPEPSVRVLREV
                     GEWMAVNGHSIYDSDPCQVRRSNYASFTRIGNTLYMHVHFWPGEYVAISGLMTKVKSA
                     KLVRTGATVPFKQDDFRVRFTDLPAKAPDSPVTTIEIECESEPTQDTDFVRRNKPRMG
                     V"
     gene            7085..9298
                     /locus_tag="VHE33_00760"
     CDS             7085..9298
                     /locus_tag="VHE33_00760"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HVT96004.1"
                     /translation="MRRNGVTGFRKAVILTPVLCAGMLATALCHGSVLTHRTRIQELQ
                     ALPLHASVHIIGMVTYVDAPGKLFWVQDETGAMPITVNPIPAGVRAGAAVSVLAGKTA
                     SLALEHVHVEPTTVGAVVAAFGEKAFPPTTAGLGPWHLAEGSVIRLTGVTSAQVAQNG
                     LIVVQQDPATLQIERRWPADLAMVQHGPVWTPPAIPAALTIVFLMAFVFLLWVYILRR
                     RVRRQMDALEKASETHKALREISSAMMAQVSTQREFTTQVAVQGGEEIAELVTGVNTI
                     LSELHRRERARREAETRLQHMALIDELTGLPNRRLLADRLSQSLALARRENRKMALLY
                     IDLDGFKLVNDSLTLHIGDLLLRQVAHRLKARFRQSDTVARIAGDEFALILDQVQTWT
                     DADVAAQGVLEVLKEPFEIEDHVIRVTASIGISLFPDHGNEGGQLLQQADCAMFAAKR
                     NGKNRIVHYGDGLGSAARERLTLEGELRRAIENGDIDVSYQPEFDLSTNAIVRFEALA
                     RWTHPKLGRIPPGSFIPVAEESGLIVPLGAYVMERACADATTWQRKAGRPIQVAVNVS
                     SVQFARDTFLEEVEEVLRRTGLKPSLLQLELTESATLTGLERAADLMRRLTGRGIGIA
                     MDDFGTGYSCLSYLPKLAFDALKLDRSFVNELAVRPETRPFVQSILSMAHNLHMRVIV
                     EGIETREQLDLMRSLGTDEAQGYLLGRPTPNPMELLSPNGDAARSIREIEAVAGR"
     gene            9425..12394
                     /locus_tag="VHE33_00765"
     CDS             9425..12394
                     /locus_tag="VHE33_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HVT96005.1"
                     /translation="MLAAIAAAAPASFALDPAKAITQYMQTSWTSESGLPQNTVQTLT
                     QTRDGYLWFGTQEGLARFDGVRFTNYTRHNAPGLASSFIQVLAAGRDGSLWIGTDSGL
                     SHYSPGSSSALSGAFTTLTTRDGLAANNITALREDRNGSLWVGTGKGLDQIFDGHIRS
                     WATSDGLADLSISAIAMDAGGTLWVGTAKGLCRFANGRFQPITSHDGFPTDGVSALAE
                     APDGSLWIGTRAHGLLQIREGRVDSFAPTLPRNEIDGLMIDHDGALWIAFDRHGIARV
                     YHGNVEIYDTKRGLPSERCTGGLFEDREGSLWVGFADAGLAQLRDGKFTVFGTPEGLS
                     GNYIGNVLQAQDGSMWIGADDKGLNHLLADGHVEVWGPQKALPNQAVYSLLQTRDGDV
                     WVGYRQGTLARIHRGQVSVFHDSQATDSSINALLEDRAGTLWIGFEGKGLAHFDHEGF
                     HHLTESGRVAALAQSADGAIWVGLDGDGLQRLDQGATVAYTTANGLHSDHVMSLFADP
                     DGSMWVGTQSGGLSHIQNGRVVTWTPDQDMPDTSIGSIIEDDSGNLWFGGDTGIFRIA
                     KQGLRQTVVPGPLHPVMYGTADGLRSRETLYGSMPCAWKSRDGRLWFGTIEGLAVIDP
                     DHIPVNTVVPPVWIERVQFDSRTIARQNGIRLGPGSGNLEFSFSAPSFVSPQQERFRY
                     RLIGFDADWIYSGTRRSAWYTNLPPGNYKFTVQAENNDGVWNESGASLSFSLRRPLTR
                     TPLAYAFYGLLALLLAWLIVAFRTRALTRRQEELTRIVAERTAQLEAEKAALEATRRE
                     LHIQATHDALTGIFNRAAMLEHLQREIARATRDRSPLGVLIADLDHFKSLNDSYGHLC
                     GDDVIRESAARFRSAMRGYDIVGRYGGEEFLILFPGWDLSMAPNRVNDLLESIRSHSF
                     EFGDCRIRLTCSIGVATFRPEVDSPSIREVLCRADTALYVAKNSGRNCASFEARSQPL
                     TTARIHTMPGLAVDKQN"
     gene            complement(12413..13540)
                     /gene="argE"
                     /locus_tag="VHE33_00770"
     CDS             complement(12413..13540)
                     /gene="argE"
                     /locus_tag="VHE33_00770"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HVT96006.1"
                     /translation="MKTVVEVLGDLVRIPSSSAVSNRPIVDYASSILRAADWSLRELP
                     YTDPGGVEKLNLIAAPPGQDPDRSDVDLVFMCHTDTVPYAADWADALNPILIDGSLHG
                     CGACDVKAVLACLLAIISRSPSPKWPDGLRIVLTADEEIGCIGAARLFSGGRLRPRRL
                     VIGEPTQLHPARAGKGYCLAEVMVHGKEAHSAHPQQGISAISNAARLITAIGKLGREL
                     AAHSHSFFDPGFTTINIGTIEGGTAKNIVPGLCRFQLEWRPIPGESASRVSEAIEQIV
                     GHLRREDPGFHAEVKPLREQPGFETAADADLVRRFEGLTGKPATSIPFGSEASIFAPL
                     ADEVVVFGPGDMRTAHSSRECVPIAELDAAVECLGSVIAGD"
     gene            complement(13537..14937)
                     /locus_tag="VHE33_00775"
     CDS             complement(13537..14937)
                     /locus_tag="VHE33_00775"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF006681.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HVT96007.1"
                     /translation="MTTLYRPELLLAGGTFLRDRGLLVGDDGRIIGEAMSDTTSDTVV
                     NLPGKALLPGFVNVHSHSFQRLIRGRAESRAVAGKDFWSWRGMMYHAAAHLEPQQLYD
                     VACMAFLEMTLAGTTTAGEFHYLHTAPNGRAYDDPNLLGKTVIAAAQSVGIRIVLLRC
                     AYLRSGFQLPRDPGQTRFFETRQEFLANMDALLREVPASASDNVRLGVAPHSIRAVPL
                     DDLREIASWARAKDLPIHMHMAEQVAENEACLREYGTTPVALAGKAGLLGPDWTAVHA
                     THITDDEIAIVAEAGATIGACPTTERNLGDGILAADKVMQAGVPVALGSDSQAQIDPL
                     EDARELDYHLRLTHQQRAVLDQIGGHSLAARLFSCATANGARALGIPAGTLAPGGFAD
                     FFTIDMRDISVAGHTRDHLLPLVVFGMNRAAIRDVVVNGKLVIRDGRHPLQDEIVNRY
                     EEVHAKVWRDAVGSER"
     gene            complement(14934..16160)
                     /gene="hutI"
                     /locus_tag="VHE33_00780"
     CDS             complement(14934..16160)
                     /gene="hutI"
                     /locus_tag="VHE33_00780"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HVT96008.1"
                     /translation="MKADLLVRGISQLVTPQGPGPKRGQAMRELKLLDDAALAMSDGK
                     VLWVGPVESWSGEAAATVDLGHRAVMPGLVDPHTHAVWAGDRLRDFEARISGVPYEKI
                     LAAGGGIWSTIRATSGASKDEMLALAKPRIDALVHSGATVIEVKSGYGFTSDKEIAML
                     EAIQCLSEMTTALIVPTLLIHVPPPSAADRGAYLSDVCQTLIPEAARRKLASAVDVFI
                     EKEAWSAAEAEKIFASARQHGLAIKLHTEQFHRVGGLELGLGLGALSVDHLEACSPEQ
                     VARMSGSNTVATILPGVSLHLGLPPAPGRALIDAGAAVAIGTDLNPGSSPLFSVSAAL
                     ALAVRLNGLTAQEALVAGTVNAAAALGVSDAGRIESGLPADFLVLNGSDWRDLVYTLG
                     SNPVREVWIRGQKVVA"
     gene            complement(16157..17815)
                     /gene="hutU"
                     /locus_tag="VHE33_00785"
     CDS             complement(16157..17815)
                     /gene="hutU"
                     /locus_tag="VHE33_00785"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HVT96009.1"
                     /translation="MSQEESSIRAPRGSTRTARGWIQEAAKRMLMNNLDPAVAEKPAE
                     LIVYGGRGKAARNWECFHKIVQTLDRLENNQTLLIQSGKPVAVLETQRLAPRVLIANS
                     NLVPHWATWEQFDKLDQAGLMMYGQMTAGSWIYIGTQGILQGTYETFQSAAQRHFSNT
                     LAGTITVTAGLGGMGGAQPLAVKLAGGVSICVEVDANRIQRRLDTRYLDKATSSLDEA
                     ISLAHKAKAARHAVSIGLLGNAAEVLPKMVEIGFTPDLVTDQTSAHDPLWGYLPIARA
                     DEDLNLVRAKQQDVYMERVRSSIADHVGAILTMQQRGAVAFDYGNNLRTQAQIAGVSD
                     AFSYPGFVPAFIRDSFCEGRGPFRWVALSGDPADIAKTDQALLQLFPDDYRLTQWLTY
                     AADQIAFQGLPARICWLGYGQRDRAGLLFNQMVRDGRLSAPIVIGRDHLDAGSVASPF
                     RETEAMRDGSDAVSDWPLLNFATGIASGASWMSFHHGGGVGMGYSQHAGLVAVADGSD
                     EAEERLRLCLRNDPALGVVRHADAGYESAIRIAQERGVDMPSLQ"
     gene            complement(18004..21792)
                     /locus_tag="VHE33_00790"
     CDS             complement(18004..21792)
                     /locus_tag="VHE33_00790"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT96010.1"
                     /translation="MTRRCIAVAALVFSLVFLAGTSIAQIATTSLRGVIKDPSGAVVP
                     NAKVTLANAANGQTLTATSNGEGAYAFPQLTPAHYVITVSATGFGDQKKTAELLVNQP
                     ATVDFSLTLQSTAQVVNVSAEAQTLNTTDASLGSSMNNALIQGLPSETRNVPDLLSLQ
                     PGVLYLPSDQGGVGASETNPMGDSRSGAVNGVRSDQGNVTIDGVDDNDQVFGYAFTGV
                     LRETQDSVEEFRVATSNTNSDEGRSAGAQVSLITKSGTNKFHGSAYEYNRPTLTVAND
                     WFNKKAELDSGQSNTPPKLIRNIFGGTLGGPIIRNKLFFFGNYEGQRRAEDTEVTRTA
                     PTKAYQQGYLTYVGDDSNGNPVTVQLRPSDVTLLDADCVTSGGCSNSQYPNGPGPNPN
                     ALAYFNSMPAANGTTVGDGYNTGSYTFASPNPYTQNTSIARFDFTPNDKHRIFVRGSL
                     QKDTLIGVEQFPGQGPSSYFEDNTKGIIAGDTWSISPNLVNDIRYGYVRQGNSTRGVG
                     SGDYVDFRFMSNSTAETRTTLATVPVNNIVDNLNWNKGKHDFQFGVNWRLVHQNRTSD
                     ANSYSGATSNPYWLSGNPPDPNAILGQPVVDGGFGNSWGIAYANLVGTVPEVDAQYNY
                     KLTSSTSGNLLADGDYVTRNFSANEFEYYLQDAWKPKPNLTITFGIRHTLLQTPWESG
                     GQEVTPTVDTDTWYKTRETEALHGQVYEPDLTFAPAGPFYGKKGFWPMSKNNIAPRLA
                     IAWAPTSKTTVRAGAGMYYEHFGQGLVSLFDQSGAYGLSGLVTNPAGTYGYEDSPRYT
                     GRSTLPFSNGTFASPVTYPFTPPTGPDLGFAITWGLDSQMKTPYTEAFNLSVQHQFAG
                     GFTLEADYVGTMGRHLIQSLDLAEPVDYVDPAGGGDYFGAGAALSKLVDQNGGDGSAS
                     VPAIKYFEDVFPWMADFDYPGESATQAIYTNEWSYYRSQYGATSALADLDFYGPASSG
                     FGFYPAPPDWQPHFWQGQFSSLYALSSIGMSYYNAAQLILRHPMSHGLQLDVSYTFSH
                     SIDEGSDAERSTEFSTSLANSPGILNTWKPWLNRASSDFDTRQLLTVDGLYQLPFGRG
                     KAFLGNANHVVDAFLGGWQLSGLSRTTSGLPFSLFEPGWTTDWQQSSFGVVTGPVKMH
                     KHFDQNGDPQYFDNVSAINSGVNTGGPIRLPYPGEAGERNYFRGDGYFDIDGGLSKTW
                     GLGEYGSLKFDWETFNVTNTVRFDPELIGSSLTSGNLGIASTLLTQPRRMEFALRYDF
                     "
     gene            22193..22864
                     /locus_tag="VHE33_00795"
     CDS             22193..22864
                     /locus_tag="VHE33_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HVT96011.1"
                     /translation="MPDSCEPTPPSPLSQQTDSGKVGISRRGFLKGAGLTAAGTALLE
                     GVQGFATEARGDSVQGVTELGPGPAAIRLHVNGTEHTVQIEPRTTLAEALRIHLGMTG
                     TKIVCDRGVCSGCTVWLDKMPVNSCMTLAIDAIGHHVTTIEGLSTEEKVHPLQAAFVK
                     HDALQCGFCTPGMVMSCAALLERNPHPSEHDVRHAVSGNLCRCGTYPKVFAATLDAAG
                     TLRNA"
     gene            22878..25268
                     /locus_tag="VHE33_00800"
     CDS             22878..25268
                     /locus_tag="VHE33_00800"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HVT96012.1"
                     /translation="MTKEPIEDAITAQVAAPAKKTVQMPVGVPGYTLAEESRSVPATE
                     PPVWPTNKDLKYVGQSIERYDGQLKASGRARYTSDIQLPGMLYAKFVNATVPHAKIVS
                     IDTAAAEKYPGVKGVHVIQHVLGNAELRNPALEGQVKYPVVRYAGQPIAAVAATTPYA
                     AEEAAALIKVKYQAMPFVVDDVEARHPDAPNVFPGAADEAGSAGGGGGPNDVKQTGNV
                     HGPSVHKHGDTEQGFKDADAVVEAHYVTQVQTHSALETHGVVADWKPDLLTVYASTQG
                     TSGVREELAGFFKIPLTKVRVITEYMGGGFGAKFGAGNPGVVAAALSKKTGAPVRLML
                     DRSEEHLSVGNRPSSDQVLRIGAKKDGTLTAIHLLAYGTAGCGTGAGCAGPAQNLYQT
                     ASLHTEENDVFINAGPGAAMRAPGHPQGAFALEQTMDELAVKLSMDPIDLRDKADSHP
                     VRRVERQIVRESDLWKSRNPVAGSDKGPVKRGVGLAQSVWYRFVSMGSNAEVRIHKDG
                     SLEVLSAVQDIGSGVRTVMAQILAEQFGVPPSQISVKVGDTTYPEGPQSGGSVTTLSL
                     TPAVRDAAWQASQKFLADIAPAFGTTADDLVLIDGEVRSQSGKFTPISFKRAASKMSS
                     SQIAVHAKRVPDYDPKKYLTYGGVDVAEVEVDTELGRIHVRRVLSVHDCGRPMNPAQT
                     ISQINGGIIQGISFTLFENRLLDPKYGLMVNPDLEQYKIAGSREIPEIDVRLVEAYIG
                     QSSTDASGIGEAAGVVSIGAAIANAVYNATGVRVRSTPMTPAVVLAALRTQGKEMA"
     gene            25265..26266
                     /locus_tag="VHE33_00805"
     CDS             25265..26266
                     /locus_tag="VHE33_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HVT96013.1"
                     /translation="MNGFSIADCTTVNAALSQLKDGAVVKAGGVDLLDRMKDGIQQPD
                     RLVNIRNVAELRGIRETDQGLTIGPLATLAEISDHPHIREHYQILADACGHAATPHIR
                     NMATVGGNLLQHVQCWYYRSSDFQCIRHGKDLCFAFEGLNQYHAIMEYSTCPSVAPSS
                     AAVALLGLGASVELTSAKRGKRTLGIRDLYIVPDANPTKFTVIEPDELITAILIPKPA
                     PGTRSAYQKYGEKESFDWAIADAGVVLEMDGDICKRAVITMGAASPVVRRSSQAEAAL
                     VGKPINEETARAAGKVSVEGATPLSQNAYKLDLFPVAVYRTVLLAAGKMDRDPSAAG"
     gene            26277..26495
                     /locus_tag="VHE33_00810"
     CDS             26277..26495
                     /locus_tag="VHE33_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96014.1"
                     /translation="MSTINATQPCQCLRSKNPYGSTPLNAESWLPSFHSVTTYWCMRT
                     MGPAGPDDHFVHLSRCKPGRPCYEERES"
     gene            26588..27292
                     /locus_tag="VHE33_00815"
     CDS             26588..27292
                     /locus_tag="VHE33_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HVT96015.1"
                     /translation="MGNSPTASMVTLHPRGILFDMDGVLVSSLGSVERSWETWAQARG
                     IDPELTIRTAHGRRAIETVRLLRPDLDEMKELEWLEEMECADKVGLETLAGVERILEN
                     LPDKYWTVVTSATERLARSRMEAGGIRVPKEIISADIVTQGKPHPEPYRRGAELLGLP
                     PSDCLVIEDSSSGALAGHEAGCKVLATLYSHSFESLRHADWIVPSLEDVHVQVLADRL
                     EVRFTPMERSAAIAAR"
     gene            27323..28036
                     /locus_tag="VHE33_00820"
     CDS             27323..28036
                     /locus_tag="VHE33_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96016.1"
                     /translation="MARSVRVEYADQRKMRTRNTRRYRAAHWPIWIWVFFLAPGPLTF
                     NLFDRGGNRWNWIWLALVLVGTGIAALFGQLPGAEPAPYILRFTEDRPNPLYRRICYT
                     FAWNALLNFALLNLAGLAWAVASGQWRLKQIYAHGYSPILIAVVLLGAFGILPRVRRS
                     TQGEGWERRYFYGAVWSVTAAQTILLILWKALPKNHATDIAKLLLYASVLVFVGVLAA
                     WGVLPRTRPILPGELMVAD"
     gene            complement(28287..29411)
                     /gene="hppD"
                     /locus_tag="VHE33_00825"
     CDS             complement(28287..29411)
                     /gene="hppD"
                     /locus_tag="VHE33_00825"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HVT96017.1"
                     /translation="MATALQSEVAAQKDFLPLNGTDHVEFWVGNARQAAYFYRLAFGM
                     KLVAYAGPETGIRDRASYVLEQGKVRFVLTTPLRPDGDIAEHVRVHGDGVHDVALWVD
                     DARQAWKETTKRGARSVREPFELKDDNGMVRISTIAAYGDTVHSFVERSDYRGAFLPG
                     YRAMPEDPLARPVGLLHIDHMVGNVGWNEMNRWVDFYAQIMGFSLYQHFDDKDISTEY
                     SALMSKVMANGNGRVKFPINEPAEGRKKSQIEEYLEYYHGPGVQHIALATSDILETVA
                     KLQAQGVQFLTVPHSYYSELEGRVGKIDEPITELEKLGVLVDRDDEGYMLQIFTKPVE
                     DRPTLFFEIIQRKGSRSFGKGNFKALFEAIEREQASRGNL"
     gene            29703..>31677
                     /gene="ligD"
                     /locus_tag="VHE33_00830"
     CDS             29703..>31677
                     /gene="ligD"
                     /locus_tag="VHE33_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HVT96018.1"
                     /translation="MPRKDHKPAKKPARQKETSAGLVDEQLARYRAMRDFAMTAEPSG
                     ASGNRTKKKLEEGLPFVVQKHAATRLHYDFRLGWKGVLKSWAVTKGPSYVVADKRLAV
                     EVEDHPMEYGGFEGTIPKGQYGGGTVMVWDSGTWEPVTDVDEGLQKGQLKFILHGKKL
                     KGHWALIRIKNNKFGDKGKNNWLLIKEHDEYERSAGDPAITDEAPDSAVTGRSLEQIA
                     DAEDHVWNSHVPEKKAPVNRSRLTRRPESSTTVEQPVPGLVKKAPVPDRKSVLKDAPA
                     EKMPRFLSPELASSVDQPPEGDDWLHELKLDGYRIQIHLDEKDGKRRARLFTRKGLDW
                     THRMPDVARTATQLPVHAAVIDGEVVVVNEKGLTSFADLQAAFDEGAPHPLTYYAFDL
                     LHLDGHNLRNLPLERRKTILEGLIQQHGEDGIRYSSHLRANGGEMLRHACQMGAEGIV
                     SKLATSTWRGERSRLWLKSKCILQQEFVVGGFAPLATGAYGIGSLLLGYYEDGELIYA
                     GRTGTGFNQKTHKMLRDRLEELRQPKPAYKSLPTEAKKDAVWVKPTMVIEARFSNWTS
                     DHLVRQAAFLGLREDKDPKEVVREVAESIPRLSRKHTNSETAQPSTPHHDAKKAKHSV
                     PAGTAAKVESPRPSKSPNRTQVAGLSITHPDKIL"
BASE COUNT         6517 a         9504 c         9531 g         6125 t
ORIGIN      
        1 ccggaacccg gtggttcgta ccgcatcccg gtagagcgag gagatttgcg catgcccctt
       61 gtgaggtaga acaatcagga agcaatcgca ttcatgaaga ttccaccact tttcgttcgc
      121 gctcatccga aggacaacgt cgccattgtg gtgaacgagg ggggattgcc tgcgggcagc
      181 cggtttgagt cggggctggt gctcgcagag gcggtgcccg aggcaaacaa gattgctctg
      241 acggacctgg ctgagggcgc ggcgatcatt cgttatggag agatcattgg acacgcacag
      301 cgtgcgctcc ctgccggcag gtgggtgcgc gatgatctgg tgcggatgcc ggctccgcca
      361 agtctcaatt ccctgccgat cgcaaccgcg gtgccgaagc ccttgccgcc gcttgaaggc
      421 tgcaccttcg atgggtacgt gaatgaggat ggctcggtgg ggacccgcaa cattctgggc
      481 atcaccacga cggtgcaatg cgtcgcggcg actgtggact acgcggtgcg gcggatcaag
      541 tcggacctct tgccgcgtta tccaaatgtg gatgatgtga ttgcgctcac ccatggctac
      601 ggttgcggga tcgcgatcga tgcgccgggt gcggagattc ccatccgaac cctgcgcaac
      661 ctgagcctga accccaacct cggcggtgag ccgatggtgg tgagcctggg atgcgagaaa
      721 ctgcagccgg taactctgat gccttcgaat acgctgccgg tgctcgaaaa cagaccttac
      781 atcgtccgat tgcaggacga ggagcatcgc agctttggag atatggtgac cgccatcatg
      841 cgcatggcgg aggaacggct gacgcggctg aacgcgcgcc gacgcacgcg gcgtccagcg
      901 tcggacctcg tggtgggcct gcagtgcgga ggcagtgatg ccttttcagg tgtgacggcg
      961 aatccggcac tggggtatgc ggccgatctg ctggtgcgtg caggagccac ggtgatgttc
     1021 tccgaggtga ccgaggtgcg cgatgcgatc cacctgctga ccgcgcgagc caccgacgaa
     1081 gctgtcgccc aggatctgat tcgcgagatg gcgtggtatg acgactactt gcgacgcggg
     1141 gcaacggatc gaagtgcgaa cccaagcccc ggcaacaaga agggcggtat ctcgaatatt
     1201 gttgaaaagg cgctggggtc agtcgcaaaa gcggggtcga cggcgctggt gggcgtggtt
     1261 ggcccaggtg agcgcgtgac cagaaaaggg ctggtgttcg cggcaacacc ggcgagcgat
     1321 tttgtgtgcg gcacgctgca gttggccgcg tccatgaaca tgcacgtgtt tacaacgggt
     1381 cgtggcacgc cgtacggctt ggccatggcg cccgtgatca aggtggcgac tcgaacgccg
     1441 ctggctgaac gatgggccga cctgatcgac gtggatgcag gaacgattgc gaccgggcaa
     1501 gccagcatcc gtgaagttgg caagcagatc ttcggaatga tcctcgacgt ggcaagcggg
     1561 cggaagcaga cgtgcgcgga ccggtggggc ctgcacaacg acctggtgtt gttcaatccg
     1621 gcgccgatca catgaagatc tgccgagcaa cgtccggtta gttcttttcc ggcgcaaagc
     1681 ttcccacaac ccgagcgcgc aggtcgcgat gcacgcgaag tgacgcggaa atctcggcgc
     1741 aggtacggcg caggtgctcg gcaagggccg gaacctgatg tggctggatg cgcgaggaag
     1801 cgcaggagac gctaagagcc gcgatggggt tgtgatcatg atcgaaaatg ggagcaccaa
     1861 tacaccgggc gcccagttca acctcctcgt catcgatgct gtagccgcgg cggcgtacgc
     1921 gctgcaaagc ctgaaggagt tcgccgcctg acccaatggt tttgggagtc aggggttcaa
     1981 atgtcatctg atcgatcagc tggcgaacct cggtctgcgg cagccaggcg agcatagcct
     2041 tgcccatagc ggtgcagtag gcaggattgg aactaccggt cctggagcct ttgcaaacgc
     2101 gagtggcggg accggccttg tcgagataaa cgacggaggt ccgctgcaga acaccgagat
     2161 gcacggtttc acccagcctc agcgagaggg agcgaaggga tggctggatc tgcctacgga
     2221 ggtccatctg ctcgacggcg cgactgccga gggtatagag cttgaggccg aggcggtagc
     2281 ggtcctccgg cgtgcgctcg agcagcatac ccctttcgag caccataagc gcgcgatggg
     2341 ctgtgctctt atgcaggccc atgctgcggc agatatcggc gagactcaga gggacatcgc
     2401 tttcgctgat gtactcgaga acggagagcg cgcggtcaag gcactgcagg cgatagggat
     2461 cgcgaagcga gctggtgtct cgcggtgtca ttttcatcgg ttttccctca atgctgcgag
     2521 gttgcgattc acccccggcg gcagaccacc caggtacatg taactcagag agtgactctg
     2581 cgcgaggatg gatgcgagag cggcatccgg aaagcgtgac tgccagatga ctccccagct
     2641 gatttgttcc gccgctggcg cgcccctcgt cggagtgtcc tgggcaatcc cggtggcctt
     2701 ctcaaagaag tggttgttct tcagaccatc gacgcacagg cgctcaagac gcgcgagcgc
     2761 gtggtcgcgc tccgcataga gatcgaggcc gttcacctgg ccgaactccg caatgtagac
     2821 cagcggggac aacgcgtaga ggtgataatg cagggcgcgc tggccgcgag ccatctcgag
     2881 cggcaaggag ccatcgggct gaatctgagc cacaccggtg cggtaggctg caacggccca
     2941 gccaaaaagc cgggggttat tcgcggcaac gcccgccgcc gcggcctcaa cgccggccca
     3001 gtaaaggtga ttattcttcg cgtcggagga gtcgtagaac gcgacggttt ggcgggcaac
     3061 gctgacgatc caggaaatga cgaggtcgcg gacagccggg gtctcaaggc cgctgtcgcg
     3121 aactttcaac cagctgacgg cgatggctcc gatgacccag ccctgtacgt agtaagcctg
     3181 acgcgaggac atctttcccg taagaactcc ctcacgagca gcggcttcca tatgactcag
     3241 agcgcacgtg gctgcagcgc ggctcccaga ggttcgccac gcatcggcgg catcgacaat
     3301 gacctggccg aggttcttgt acggacctga gctttcggag taagccttcc actttgccgg
     3361 atcgatgacg gaggctttgc tgtcggagta aaaaccgtcg gtagtgagat cgcgcgaaag
     3421 ataagcgatg ggcgggcagg tgtaaggctc gtcggtcggc acgatgggat ggtcgtccca
     3481 gggagaacga agaggcgagg ttgacgccga ggctgcagcg aacgcgatgg tgattgcggc
     3541 gatcgcccgc atcgtgatgg agagtccaga cctgcactta gagcgcataa tcctcacgag
     3601 agggaacgaa aaagtccagc acttccgact ctgccaccgc gctggcctcg tgctccattc
     3661 caccagggac gataaagctg tcaccagcaa atgcttcaaa gctctcgctt ccacaaatga
     3721 agcggatgtg tccgcggatc acataaacca tctgctcgtg gggatgggag tggcgggcgc
     3781 cgcgccagcc aggtcgcatc tggtggcgaa ccagcatcat gcccgggtta gaagcgagga
     3841 cctgccggat caggccatct tccggatggg tggcgttgac attttttagg ctcgtcaggc
     3901 ccttgaattg ttcgctcatc catctcctgt cgcctgcgcg gagccagggg ctcaccctgt
     3961 ttgcagttgc aggctggatg gatttttcgc gtctcgttat tggactgtca acagacgtgc
     4021 cacgaaacca tgcatcgcgt accgcatgcg gagattggct tagggtacgc gcagcaggca
     4081 aacgcaaact cttccccatc ccacgtctcg atacaagatg agggcggcga tcttgacaaa
     4141 tgaatcctac ttcgtcatct gcgcgagctc agagccggcc aggagaaatg cgccaacgcc
     4201 gtagacgtaa ctcgacgacg gcttgaattg atccggcgct gcgccgatgg gttggatcga
     4261 tccgaggcgg ccatcctggt agatgtgggt gagcatgccg cgccaggctt tggaaacgac
     4321 aggcatatag gtctttttgt cgagcagatg atggtggatg ccccacgcca tggcgtacgt
     4381 aaaaaaggca gagccggaga tttcgggcga cttgtaggct tcaggatcca ggagtccgga
     4441 gcgccacaga ccgtcggcag gctgcaaagt agcgatacgg gcggccatgt cgcgatagag
     4501 cgcgatgtac ttctctcgct gtggatagtc ctggggcatg cgttccagca ccagagcgag
     4561 gcctgcaagc acccagccgt tgccgcggga ccagaaaatc ttctgcccgt tgggctcgtg
     4621 catggtgaga aaacgatcgt cgcgatagta aaggtgatcc tgcggatcgt agagagcctt
     4681 tgtggtcaga gaccactcgt ggtccatgaa gtcgaggtag cgatggtcgc cggtcgcgac
     4741 ggagagctgc gcgaggacgg gcggcgccat atagagcgca tcgcaccacc accagaggtt
     4801 cttctgcgga tcgtcggggc ggtcgaggag cttgtcgaga ttctctttgg tgggagcgag
     4861 atggtccggc ttatgatctt cggcgtaaaa ggtgagatag gttaggccga tgtgctcatc
     4921 gtcggcatgc ccgaagcgtg gacccagctc ccagtgattt tctttggcga tctcaaaaac
     4981 gcggtcgcgg tagcgcggat cgccggtcgt acgtgaggcc gccagcatgc catcgtagag
     5041 tgccgcgtag gtccagccaa tgttgtagcg cccctcggcg tgctgaagct gccagttcgc
     5101 taccagtctc atcgccttca gaatggcggt gcgcttgaga gctggagaga gattggtggc
     5161 gaggggtccg ggattgtcag gctcgtcgcc ggctgcggct cgttccgcat tggcgggcga
     5221 gggggtgctc tgcccccatg tgggaagacc ctggctcatc gcgaaaccaa gcaggagaag
     5281 agaaagaatt ttcttcatag tcactcgctg tgaattctac agttgcgcgg acccagcata
     5341 ccgagtgtca ttctcaatcg caggaaagaa ggctgaatag aggtacgtac cccagtgagg
     5401 gacagatctg caatttgaga agaaaaaaac atccagggtc tgttgcaatg agcagaaacg
     5461 agtgcggctg agaatggcaa cggttcaaaa tcaaagtgtg gaggaagcgt gaagaatcct
     5521 gaaatcacac ggcgcagtct tctgaaaggc acaggcgcat ccctgctggc aatgggagca
     5581 ggagtgccgg caggggcacc gatgctggcg gcacaggaaa cctccgccaa ttcgactcca
     5641 ctgccgggcg acaccgatcg tgaagagcgc atgaaatggt ggaaggcggc acgcttcgga
     5701 atgttcatcc acttcggcgt ctactcaacg atcgggcggc acgaatgggt gatggaggac
     5761 gagggcatcc ccgttgtgga gtatgcccca cacgcgacga ctttccaccc ggcgcctgga
     5821 tcgcaacgcg catgggctcg cctggcgaaa caggcaggta tgaagtacat ggtgatgacg
     5881 agtaagcacc atgagggctt ctgcaacttc aatacgaagc tgacgagcta ctgcgcacca
     5941 aagcagggtc cgggacgcga cctgatcggc gaatacgtgg aggccgcgcg agccgagggt
     6001 cttcgagtcg gcttctatta ctcgctgatg gactggcatc atccggatgg cgccagctgc
     6061 gcgacaaatg aagatgcgcg caaacgattt gtcgactaca cccacggcct gatccacgaa
     6121 ctgatgtcga actacggcaa gatcgatgtg ctgtggtacg acgtggcgtg gccgctcgac
     6181 gcgcagggct gggaatcggt gcggatgaac gagatggttt tcaggttgca acccgaaatc
     6241 atcgtgaaca accgcaataa attgcccggg gatttttcga cgccggaaca gcacatcaca
     6301 gcggaaaaag gcggccgcgc gtgggaagct tgcatgaccc tgaacgacag ctggggctat
     6361 caggcggccg acgacaactg gaagtcatcg aaaaccgtga tcaggaatct gatcagctgc
     6421 gtccgggact ccggaaatta cctgctgaat gtcggtccga agccggatgg atcggtaccg
     6481 gagccttcgg tgcgggtgct gcgcgaagtt ggcgagtgga tggcggtgaa cgggcacagt
     6541 atctacgatt ccgacccgtg ccaggtgcga cgttcgaatt acgcgagctt cacgcgcatc
     6601 ggcaacacgc tgtacatgca cgtgcatttt tggccgggag aatacgtggc gatttccggt
     6661 cttatgacca aagtgaagtc ggcgaagctg gtcaggacag gagcaacggt gccctttaag
     6721 caggacgatt ttcgcgtgcg cttcacggat ctgccggcga aggcgccgga ttctccggta
     6781 acgacgatcg aaatcgagtg cgagtcggag ccaacccagg acacggactt cgtgcgcagg
     6841 aacaagccgc gcatgggagt ttgacgcgca gagtatttgc cgggccgagc cgaatcgtcc
     6901 ggtgtaataa ccaaatagcc aatccgtcct gaccctgtcg tcatcgggtg aagcaggtaa
     6961 agccactttt gcgacccctg gccttgaatg tcgacgggtt cttaagtcgc ctaattccat
     7021 tcgggtaggc cgaaaaccca aagttgtatg gctccggatg ccatagccaa ttgatcatcc
     7081 tccaatgagg cgcaacggcg tcacaggatt caggaaagcc gtgatcctga caccggtgct
     7141 gtgcgcgggc atgctggcca ccgcgctgtg ccacgggtcc gtcctaacgc accgcaccag
     7201 gattcaggaa ctgcaggcac ttcctctgca tgcctccgtg catatcatcg gcatggttac
     7261 gtatgtcgat gcgccgggga agctcttctg ggtccaggac gaaaccggtg caatgccaat
     7321 cacggtcaac cccattcccg ccggagttcg tgcgggagcg gcggtctccg tcctcgccgg
     7381 caaaaccgcg agcctggcgc tggaacatgt gcacgtggag cccacaaccg tcggcgccgt
     7441 agtggccgcg ttcggtgaaa aagcctttcc gcccaccaca gccggcctcg gcccctggca
     7501 tttggctgaa ggcagcgtga ttcgactcac tggagtcaca tcagcccagg tagctcagaa
     7561 cgggctcatc gtggtgcagc aggatcctgc gacgctccag atcgaacgac gctggcctgc
     7621 ggacctggcc atggtgcagc acggaccggt ctggacgccc ccggcaattc cagcagcact
     7681 gacgatcgtc ttcctcatgg catttgtctt cctgctgtgg gtctacattc tgcggcgtcg
     7741 cgtacgcagg cagatggatg ccctcgagaa ggcttcagag acgcacaaag cccttcgtga
     7801 gatctccagc gcgatgatgg cgcaggtgtc gacgcagcgt gagttcacaa cccaggttgc
     7861 cgtgcagggt ggcgaagaaa tcgccgagct ggtgactggg gtcaatacca ttctttccga
     7921 attgcaccgg cgcgagcgtg cccgccgtga agccgaaacc cgcctgcagc acatggcgct
     7981 catcgacgag ctgaccggat tgcccaatcg ccggctgttg gcagatcgtc tctcgcaaag
     8041 cctcgcgctg gctcggcgcg agaaccggaa aatggcactc ctgtacatcg atctggacgg
     8101 ctttaagctc gtcaacgaca gcctcacgct tcatatcgga gacttgctgc tgcgccaggt
     8161 agcccatcgc ctgaaagccc gattccgtca gtccgacact gtggcgcgga ttgccggtga
     8221 cgaatttgcg ctgattctcg atcaggtaca aacctggacg gatgctgacg tagcagcgca
     8281 gggcgttctg gaagttctga aggagccctt cgagattgag gatcatgtga tccgcgtgac
     8341 cgccagcatt ggtatcagcc tctttcccga tcacggcaac gaaggtggac aactcctgca
     8401 gcaggccgac tgcgccatgt tcgctgctaa acgaaacggt aagaaccgca tcgtgcacta
     8461 tggggatggg ctcggtagcg cggcccgcga acgcctcact ctggaaggag agctgcggcg
     8521 ggcaatcgag aatggcgaca tcgatgtttc ctaccagcct gagttcgatc tctccacgaa
     8581 cgcaatcgtt cgattcgaag ccctggcgcg ctggacgcat ccgaaactgg gacgaatccc
     8641 gccgggaagt ttcattccgg tggcagagga gagcggactc atcgttccgc tcggagcgta
     8701 tgtaatggaa cgggcctgcg ccgatgcgac gacctggcag cgcaaggccg gccgcccgat
     8761 tcaggtggcg gtgaatgtct ccagtgtgca gtttgcgcgc gatactttcc tggaagaggt
     8821 ggaggaagtg ctgcgccgca ccgggctcaa gcccagcctc ctgcaactcg aattgaccga
     8881 atccgcaact ctgacgggac tggaacgtgc cgcggacctg atgcgcagac tgaccggcag
     8941 aggcatcggt atcgccatgg acgacttcgg caccgggtac tcctgcctga gctatttgcc
     9001 caagctcgcc ttcgatgccc tgaagctcga tcgctcgttt gtgaatgaac tcgcggttcg
     9061 tcccgagact cggccttttg ttcagtccat cctgtccatg gcgcacaact tgcacatgag
     9121 ggtgatcgtt gaagggatcg agacccggga acagctggat ctgatgcgat cgctgggaac
     9181 agacgaagcc cagggatatc ttcttggccg ccccacgccc aaccccatgg aactgctgtc
     9241 ccccaacggg gacgcggcac gatcgatccg agagatcgag gcagtcgcag gacgctgaac
     9301 tccgatggct ccgatttcct cagctcacca tgaagagtgt gcccgatccg cgagcggcct
     9361 gccgctgcgg cgttccggga agagacacgt cggtgcgagg cgagtcgcga tcctggctgc
     9421 ttcagtgctt gcggccattg ccgctgcggc gccggcgtca ttcgctctgg atccggcaaa
     9481 agcgatcacc cagtacatgc aaacttcgtg gacgagcgag tccggtttgc cacagaacac
     9541 tgtacaaacc ttaacgcaga cgcgcgacgg atatttgtgg ttcggaaccc aggaaggcct
     9601 cgcccggttt gacggcgtcc gcttcacgaa ctacacccgc cacaatgccc ccggcctcgc
     9661 ctccagcttc attcaggtac tcgccgccgg acgcgatggc agcctctgga tcggaaccga
     9721 cagcggtctc agccactact cgcccggatc gtcatcggca ctgagcggag cgttcaccac
     9781 attgacgacc agagatgggt tggccgccaa caacattaca gctctccgcg aggatcgcaa
     9841 cggcagcctc tgggtcggta cgggcaaggg cctggaccaa atctttgacg gacacatccg
     9901 aagctgggcc accagcgacg gacttgccga tctgtccatc tccgcaatag cgatggacgc
     9961 aggcggaacg ttgtgggtgg gaacggccaa agggctgtgc cgattcgcga acggtcgctt
    10021 ccagccaata acttcgcacg acggttttcc caccgatggc gtctccgccc tggccgaggc
    10081 tccggatggc tcactgtgga tcggaacgcg cgcgcacggt ctgctgcaga ttcgcgaagg
    10141 ccgcgtcgat tctttcgcgc cgacgctgcc gcggaatgaa atcgatggac tcatgatcga
    10201 ccacgacggt gctctctgga ttgcgtttga tcgacacgga attgctcgcg tctatcacgg
    10261 caacgtcgag atttacgata cgaaacgagg tcttcccagc gaacgatgca caggtggctt
    10321 gttcgaggat cgcgaaggca gcctgtgggt cggcttcgcg gatgcaggcc ttgctcagct
    10381 ccgcgacggt aaattcacag tattcggaac ccccgagggc ctttccggca attacatcgg
    10441 caatgttctc caggcacagg acggcagcat gtggattggc gcggacgaca aaggcctcaa
    10501 tcacttgctg gcggacggcc acgtcgaagt atggggacca caaaaggcct tgccgaacca
    10561 ggcggtctat tccctcttac agacccgcga tggcgatgtc tgggtcggct accggcaggg
    10621 aaccctggcg cggattcacc ggggacaagt ctccgtcttc cacgattccc aggccactga
    10681 ttcttccatt aatgcgcttc ttgaggatcg cgccggcaca ctctggatcg gatttgaagg
    10741 caagggtctg gcgcacttcg atcatgaagg atttcaccac ctcacggaga gcggaagggt
    10801 cgcagccctg gcgcagtctg ccgacggagc aatctgggtt gggctcgatg gggacggcct
    10861 gcagcgtctc gatcagggcg cgacggtcgc ttacacaacc gccaacggcc tgcacagcga
    10921 tcacgtgatg agtctcttcg ccgacccgga tggaagcatg tgggtgggaa cacagagcgg
    10981 ggggcttagt cacattcaga acggccgcgt tgtaacgtgg acgccggacc aggacatgcc
    11041 ggacacctcc atcggctcga tcattgaaga cgactccggc aatctatggt tcggtggcga
    11101 tacgggcatc tttcgcatcg caaaacaggg gctaaggcag acggtcgtgc ccggacccct
    11161 tcatccggtg atgtacggca ccgccgatgg cctgcgctcc cgcgagacgc tgtatggcag
    11221 catgccatgc gcgtggaaga gccgagacgg ccgtctctgg tttggcacca ttgaaggatt
    11281 ggcagtcatc gatccggacc atatcccggt gaataccgta gtaccgccgg tgtggattga
    11341 acgcgtgcag ttcgactcgc gcaccatcgc ccggcaaaat ggcattcgcc tcggaccggg
    11401 ctccggcaat ctggagttct ctttcagcgc tcccagcttt gtatcgcccc aacaggagcg
    11461 ttttcgatat cggctcatcg gtttcgatgc ggattggatc tactccggaa cccgtcgcag
    11521 cgcatggtat acgaacctcc ctccaggaaa ttacaaattt accgttcagg ccgagaacaa
    11581 cgatggagtc tggaatgagt ctggagcttc gctcagcttc tcgctgcgcc gtccgctcac
    11641 ccgcactcct ttggcctatg cattctacgg acttctggcc ttgctgctcg catggttgat
    11701 cgtcgctttt cgcacgcgcg cgcttacccg ccgccaggaa gaactcacac gcattgttgc
    11761 ggagcgcact gcgcagctcg aagcggaaaa agccgcgctc gaagcaactc gccgcgaact
    11821 gcatatccag gccacccacg atgctttgac cggcatcttc aaccgtgccg cgatgctgga
    11881 gcacctgcag cgcgagatcg cacgcgccac ccgcgacaga agcccgctcg gcgtgctgat
    11941 cgcggatctg gatcatttca agagcctgaa cgacagctac ggccatcttt gcggtgatga
    12001 tgtcatccgc gagtcggcgg cccgcttccg ctccgccatg cgcggatacg acattgttgg
    12061 gcgatacgga ggcgaggaat ttctcatcct gtttccagga tgggatctgt cgatggcccc
    12121 gaatcgtgtc aacgacctgc tggaatccat tcgctcgcac tctttcgagt tcggcgactg
    12181 ccgcatccgt ctcacctgca gcatcggagt ggcgaccttt cggcctgagg tcgactcgcc
    12241 ttcgattcgc gaggtgctct gccgtgccga cacagccctc tatgtagcca aaaactcagg
    12301 gcgaaactgc gcgagctttg aggcacggtc tcagcctctc accacggcgc gcattcacac
    12361 catgcccgga ctggctgtcg acaaacaaaa ctagccttgc tcgtcgcaga aatcagtcgc
    12421 cggcaatcac cgagccgagg cactctacag ctgcgtccag ttccgcgatc ggcacacatt
    12481 cgcggctgct gtgtgcggtg cgcatgtcgc ccgggccgaa gacaacaact tcgtcggcaa
    12541 gaggtgcgaa aatgctcgct tccgaaccaa acggaatcga tgtcgctggc ttgcctgtga
    12601 gtccctcgaa gcggcgtacc agatctgcgt cagctgctgt ctcgaatcct ggttgttcac
    12661 gcaaaggctt gacctcagcg tgaaaacccg gatcctcgcg acgcaggtga ccgacaatct
    12721 gctcgatggc ttcagaaact cgactggccg actcgcccgg aattggacgc cactccagct
    12781 gaaagcggca taacccgggt acgatgttct tcgcagttcc gccctcgatc gtccctatat
    12841 tgatcgtcgt aaagccagga tcgaaaaagg aatgggagtg cgccgcgagt tctcgaccca
    12901 gttttccgat cgcggtaatg agcctcgcgg catttgaaat cgccgaaatc ccctgttgcg
    12961 gatgcgcgct gtgcgcttct tttccgtgaa ccatgacttc ggccaggcaa tagcctttgc
    13021 cggcgcgcgc gggatgcaac tgcgtcggct cgccgatcac caggcgccgt ggccgcagcc
    13081 tgccaccact gaagagacgc gccgcgccga tacatccgat ttcttcgtct gccgtgagaa
    13141 cgatgcgaag gccatccggc catttcggcg aagggcttcg tgaaatgatc gcaagcagac
    13201 acgcgagaac cgccttgacg tcgcaggctc cgcagccgtg cagagaacca tcgatcagaa
    13261 ttggattgag tgcatcggcc caatcggcgg catacggaac tgtgtcggta tgacacatga
    13321 agacgaggtc gacgtcggat cggtccgggt cctggccagg cggtgccgcg atcaggttca
    13381 gcttctcgac cccacccgga tcggtgtacg gtagttcgcg caatgaccag tctgccgcgc
    13441 gcagaatcga actggcatag tccacgattg gccggtttga taccgccgaa ctggagggaa
    13501 tgcggaccag gtctccgaga acttcgacaa ctgttttcat cgttccgatc ccaccgcatc
    13561 gcgccagacc tttgcatgca cctcttcgta gcggttgacg atctcgtcct gaagagggtg
    13621 gcggccgtcg cgaatcacga gctttccatt cacaacaaca tcccgaatgg ctgcccgatt
    13681 catcccaaag accaccagag gcagtagatg atcccgcgtg tgaccagcga cagaaatgtc
    13741 gcgcatgtcg atggtgaaga aatcggcgaa accgccgggg gcaagagttc cggcaggaat
    13801 gccaagcgcg cgtgcaccat tcgcggttgc gcagctgaac aaacgcgccg ccaggctgtg
    13861 accaccgatt tggtcgagga ccgcgcgttg ttgatgcgtc agacgcagat ggtaatccag
    13921 ttcccgcgcg tcttccagcg gatcgatctg ggcctggctg tcggacccga gggcaaccgg
    13981 gacgccggcc tgcatgactt tgtctgccgc gaggatcccg tcaccgaggt tgcgctccgt
    14041 cgtcggacaa gcgccgatcg ttgcgcctgc ctcggcaact attgcgattt catcgtccgt
    14101 gatgtgggtg gcgtgtacgg cagtccagtc cggccccaga agaccggcct tgccggccag
    14161 cgccacaggc gttgttccat attcgcgcag acaggcttcg ttttcggcga cctgctcggc
    14221 catgtgcatg tgaattggca gatctttagc cctcgcccac gatgcaatct cacgcagatc
    14281 gtcaagtggc acggcacgga tgctgtgggg agcaacaccg agtcggacat tgtcggacgc
    14341 ggacgcgggt acttcgcgca gcaaagcgtc catattggcg aggaactcct gccttgtttc
    14401 aaaaaagcgg gtctggcccg gatcgcgtgg cagctgaaaa cctgatcgca ggtaggcgca
    14461 gcgcagcaac acgatgcgga tcccgacgga ttgcgcagcg gcaatgaccg tcttgccgag
    14521 aagattgggg tcgtcgtacg ctcgcccgtt cggtgctgta tgcaggtaat ggaactcgcc
    14581 tgctgtggtt gtgccggcga gcgtcatctc aagaaacgcc atgcacgcca cgtcgtacaa
    14641 ctgctgcggt tccaaatgcg ccgcggcgtg gtacatcatg ccgcgccagg accagaagtc
    14701 tttcccggcg acagcacgcg attccgccct gccgcggatc aaacgctgaa acgaatggga
    14761 gtggacgttg acgaaccccg gcagcagcgc tttgcccggc agattcacca ccgtgtccga
    14821 cgtcgtgtca gacatggcct caccgatgat cctgccatcg tctccgacaa ggagaccacg
    14881 gtcgcggagg aaggttcctc cggccagaag cagttcgggg cgatacagcg tggtcatgca
    14941 accaccttct gtccgcgaat ccacacttcg cgaacgggat tgctgccgag cgtgtaaacg
    15001 agatcgcgcc agtccgagcc gttcaggacg agaaaatcag ccggcagtcc agactcgatc
    15061 cgtcccgcgt cggacacacc gagtgccgcg gcggcattca cagttccagc cacaagagct
    15121 tcctgtgcgg taagaccatt cagacgcacc gcaagagcga gagccgccga gaccgagaac
    15181 agcgggctcg atccggggtt gagatcggtt ccgatcgcaa cagcggcgcc ggcatcaatg
    15241 agggcgcgcc ccggcgcagg cggcaggcca agatgcagcg aaactccggg aagaatggtg
    15301 gcgacagtgt tgctgcccga catgcgcgcg acctgctctg gactgcaggc ttccagatga
    15361 tcgacgctga gcgcgccgag ccccaggccg agttcgaggc cgcccacgcg gtgaaactgc
    15421 tcggtgtgga gtttgatggc caaaccatgc tgccgggcgc tcgcgaagat cttctctgcc
    15481 tccgccgcgc tccatgcctc cttctcgatg aaaacatcga cggcggacgc cagttttcgc
    15541 cgggcggctt cgggaatgag tgtctgacac acgtcggaca agtaagcccc gcggtctgcc
    15601 gcggatggcg gcgggacatg gatcagcagt gtaggaacga tgagagcggt ggtcatctcc
    15661 gacagacatt ggatcgcttc aagcatggcg atctccttgt ctgacgtgaa gccgtatccg
    15721 gactttacct cgatgacggt ggcgccggag tgcaccagag catcgatgcg cggcttcgcg
    15781 agcgcgagca tttcgtcctt cgatgcgccg gacgtggcgc gaatggtgct ccagattccg
    15841 ccccccgccg caaggatctt ctcgtagggc actccggata tgcgcgcctc gaagtcccgc
    15901 aaacgatcgc cagcccagac cgcgtgcgtg tgaggatcga ccagtcccgg cataacggcg
    15961 cgatgtccga gatcgaccgt ggctgcagct tcaccggacc atgattcgac cggtccaacc
    16021 cacaacacct tgccgtcgga catggccaat gcggcatcgt cgagcagttt cagctcgcgc
    16081 atagcctggc cgcgcttcgg cccggggcct tgcggcgtga caagctgcga gatcccgcga
    16141 accagcagat cggctttcat tgcaggctcg gcatgtccac gccgcgttcc tgtgcaatac
    16201 gaattgcact ttcgtagccc gcatcggcgt gccggacaac accgagtgcg ggatcattgc
    16261 gcaggcagag ccgcaggcgt tcttccgctt catcgctgcc atccgcgacc gcgacgaggc
    16321 ccgcatgctg gctgtagccc atgccgacgc cgcccccgtg gtggaagctc atccacgacg
    16381 caccgctggc aatgcccgtg gcaaagttca gaagcggcca gtccgataca gcatccgaac
    16441 cgtcgcgcat cgcctcggtc tcgcggaacg gacttgcgac cgaaccggcg tccagatggt
    16501 cacgaccgat gacaatgggc gcgctgaggc gcccgtcgcg caccatttgg ttgaagagta
    16561 agccggctcg gtcgcgctgc ccgtacccaa gccagcagat gcgcgccggc agcccctgga
    16621 acgcaatctg atcggccgcg taggtcagcc actgggtcag acggtaatcg tcggggaaaa
    16681 gctggaggag ggcctgatcc gtcttcgcga tgtctgccgg atcgccggag agggccaccc
    16741 atcgaaatgg accccgtccc tcgcaaaacg aatcgcgaat gaaagccgga acgaacccgg
    16801 gataggaaaa tgcgtcggac acccccgcaa tctgcgcctg cgtgcgcaga ttgttgccgt
    16861 aatcaaacgc aacagccccg cgctgctgca tcgtgaggat cgccccgaca tggtccgcaa
    16921 tggaagaccg gacgcgttcc atatagacat cctgctgctt tgcgcggacc aggttcagat
    16981 cctcgtcggc gcgagcgatg ggcaggtatc cccacagcgg gtcgtgcgcg ctggtctgat
    17041 ccgtcaccag atcgggagtg aagccaatct caaccatctt cggcagaacc tccgcagcgt
    17101 tgccgagcag gccaatcgag accgcgtgcc gcgcggcctt cgccttgtgc gctaaggaaa
    17161 tagcttcgtc cagcgaactc gtggccttgt cgagatagcg agtgtcaagc cggcgctgaa
    17221 tccggtttgc atcgacttcg acacagatac tcacaccgcc tgcaagtttc accgccagag
    17281 gctgcgcgcc ccccatcccg ccgagacccg cggtcacggt aatcgtcccc gcaagcgtgt
    17341 tgctgaagtg gcgctgcgcc gcactctgga atgtctcgta ggttccctgc aaaatgccct
    17401 gggtaccgat gtagatccag gagccggccg tcatctgccc gtacatcatc aggcctgcct
    17461 gatcgagctt atcgaactgc tcccaggtag cccagtgcgg caccaggttt gaattggcga
    17521 tgaggacgcg cggtgccagg cgttgcgttt cgagcaccgc cactggtttg cctgactgaa
    17581 tcagcagcgt ctggttattc tccagtcgat cgagggtctg aacaatcttg tggaagcatt
    17641 cccaattgcg cgccgctttg ccgcgtcctc cataaacgat caactccgcg ggcttctccg
    17701 cgacggccgg atcgagattg ttcatcaaca tccgcttcgc ggcttcctgg atccagccgc
    17761 gcgcggttct ggtgctgccg cgtggcgcgc gaatggacga ttcttcctga ctcatcgcga
    17821 tcctcttgaa caggccattc tcaacgcttc gcgcgcgcga agcaactgcg aactctccgc
    17881 agccttttgc tggttcagag cgcccagctg taggttgggg gtcacggact aaaccccgca
    17941 gcgaaaactg cgagcaaaga aaaagcgccg gagacgagtc tccggcgctt ttggtggggt
    18001 gagttaaaag tcgtagcgga gtgcgaattc catccggcgc ggctgcgtca acagggtgct
    18061 ggcaatgccc aggttgccgc tggtcaggct cgagccgatc aattcaggat cgaatcgaac
    18121 agtgttcgtc acgttgaacg tctcccagtc gaacttcaac gatccgtatt cgcccaggcc
    18181 ccaggtcttc gagaggccac cgtcaatatc gaagtagccg tcgccgcgga agtaattccg
    18241 ctcgccagcc tctcccggat agggcaaccg tatcggccct ccggtattga cgccgctgtt
    18301 gatggcgctg acattgtcga aatactgcgg atcaccgttc tgatcgaagt gcttgtgcat
    18361 cttgaccggg ccggtgacca cgccgaagct cgattgctgc cagtccgttg tccagccggg
    18421 ttcaaaaagc gaaaacggca ggccactcgt cgtgcggctc aatccggaaa gctgccagcc
    18481 gcccaaaaag gcatcgacca catgattcgc gtttccaagg aaagccttac cacgaccaaa
    18541 cggtagctga tagaggccat ccacggttaa taactggcgc gtatcgaagt ccgacgaagc
    18601 ccggttcagc catggtttcc aggtgttcag aatgccgggc gaattcgcca gactggtgct
    18661 gaactctgtg ctgcgttcag catccgatcc ctcatcgatg gagtgggaaa aggtgtaact
    18721 cacatccagt tgtaagccat gactcatagg gtgacgcagg atgagctgcg cggcgttgta
    18781 atagctcata ccgatcgaag acaaagcata gagcgatgag aactgcccct gccagaagtg
    18841 cggctgccag tccggcggcg ccggatagaa tccgaacccc gatgatgccg gaccgtaaaa
    18901 gtccagatcc gccagagcag atgttgctcc atattgcgaa cggtagtaag accactcgtt
    18961 ggtataaatc gcctgcgttg cgctttctcc cgggtagtca aaatcggcca tccaggggaa
    19021 gacgtcttcg aaatacttga tcgccggaac agaagcgctc ccatcaccac cgttttgatc
    19081 gaccaactta gaaagcgccg caccagcacc gaaatagtca ccgccgccgg ccggatcgac
    19141 atagtcgacc ggttcagcca gatcgagact ctggatcagg tgcctgccca tcgtcccgac
    19201 atagtccgcc tccagtgtga agccaccagc aaactgatgc tggaccgaaa gattgaacgc
    19261 ctcggtgtag ggcgtcttca tctggctgtc cagtccccac gtaatcgcga agccaagatc
    19321 cggccccgtc ggtggagtga aaggataggt caccggcgag gcgaaggttc cgttgctgaa
    19381 aggcagtgtg ctccgccccg tatagcgcgg ggagtcctcg taaccataag tgcctgcggg
    19441 atttgtcacc agtccgctca gaccataggc tcccgattgg tcgaacaagc tcaccaggcc
    19501 ctgtccgaag tgctcatagt acattccagc gcccgcccgg acagttgtct tggacgtggg
    19561 agcccacgcg attgccagac gcggcgcaat gttgttcttt gacatcggcc agaaaccctt
    19621 cttgccgtaa aacggacccg ctggagcaaa ggtcagatct ggctcataaa cctggccatg
    19681 aagagcctct gtctcgcgtg ttttgtacca ggtgtcggtg tcgacggtcg gtgtaacttc
    19741 ctggccgcct gactcccatg gcgtctggag caatgtgtgc cgaataccga atgtgatcgt
    19801 gaggttgggc tttggtttcc acgcatcctg cagatagtat tcgaactcgt tggcactgaa
    19861 attccgtgtc acgtagtctc catcggccag cagattgcct gaagtcgaac tggtcagctt
    19921 gtagttatat tgcgcatcca cctcaggaac ggtgcccacg aggttggcgt acgctatccc
    19981 ccacgagtta ccgaacccac cgtcgacaac cggctgtccc aaaatcgcat tgggatctgg
    20041 cgggttgccg ctcagccagt aaggattgga ggtggcgccg ctgtaggaat tagcatccga
    20101 tgtccgattc tggtgaacca gacgccagtt cacaccaaac tggaaatcgt gtttgccctt
    20161 attccagttg agattgtcga cgatgttatt gaccggcacc gtggccagag ttgttcgcgt
    20221 ttccgctgtc gagttcgaca taaaacggaa gtcgacatag tcacccgaac caacgccgcg
    20281 tgtgctgttc ccctggcgaa cgtaaccata acggatatca ttcaccagat tcggcgagat
    20341 tgaccacgta tcaccagcaa tgattccctt agtgttgtcc tcgaaatagc tcgagggtcc
    20401 ctgacctgga aactgctcga ccccgatcag cgtgtccttc tgcagactgc cccgaacaaa
    20461 aattcgatgc ttgtcgttcg gagtaaaatc gaagcgtgcg atgctcgtat tctgggtgta
    20521 gggattcgga gaggcgaagg tgtaagaccc tgtgttatag ccgtctccaa ccgtcgttcc
    20581 attggccgcc ggcatgctgt tgaaatacgc aagcgcgttc gggttcggtc ccggaccgtt
    20641 gggatactgc gaattcgaac agccgccgga agtcacgcag tccgcatcca gcagtgtcac
    20701 atccgatggg cgcagttgca ccgtcaccgg gttaccgttc gagtcatctc cgacatacgt
    20761 gagatatccc tgctgataag ccttcgtcgg cgctgtccgc gtcacttccg tgtcttcggc
    20821 gcggcgctgc ccctcatagt tgccgaagaa gaacaacttg ttacggatga tcggccctcc
    20881 cagcgttccg ccgaaaatat tgcggatcag cttgggggga gtattggact gcccgctgtc
    20941 caattccgcc tttttgttaa accagtcgtt ggccacggtc agggtaggcc ggttgtactc
    21001 gtaggctgat ccgtggaact tgttcgtgcc ggacttggtg atcaggctca cctgcgcacc
    21061 ggctgaccgg ccttcgtcgg aattcgtatt cgatgtggca acgcggaatt cctcgaccga
    21121 atcctgggtt tcgcgcaaca cgccggtaaa agcgtaccca aagacctggt cattatcgtc
    21181 gacaccgtcg atggtcacgt tgccttgatc tgatcggacg ccgttgaccg ccccgctccg
    21241 gctatcgccc atcggattcg tttccgaggc tcccacccca ccctggtcgc tgggcagata
    21301 caaaactcca ggctgcagcg acagcaggtc aggaacattg cgcgtctcgc tgggcaaccc
    21361 ctggatcagc gcgttgttca tcgagctgcc cagggaagcg tcggttgtgt tcagggtctg
    21421 tgcttccgcg ctcacattca ccacttgggc ggtggactgc agggtcaggg agaagtccac
    21481 cgtcgccggc tgattcacga gcagctcagc ggtcttcttc tgatcgccaa agccagtcgc
    21541 cgacaccgtg atgacgtaat gagcaggcgt cagctggggg aaggcatatg caccctctcc
    21601 attggacgtt gcagtcagcg tttgaccgtt cgctgcgttc gcgagtgtca ctttggcatt
    21661 cgggactact gctccgctcg ggtccttgat gactccccga agcgaagtag ttgctatttg
    21721 ggcaatgctg gttccggcaa ggaacaccag cgagaatacg agcgcagcaa ccgcgataca
    21781 gcgtctggtc acgtttcctc caaaatgctt gctggtgccg cgtgggtcgg cacttcgttg
    21841 ttttcaatct cagctctgtg acggattaca gcagtggccg gatgagtaaa ctattttgcg
    21901 gcatataagt tacaacttca agggacgagc gccgaaattg gctcaggaaa tccacgtcgc
    21961 ataagcgatc tttgttgccc gcatcaggtg cgcgtgccgc cgttgtggga tgaacgatga
    22021 ctcatcagtg acgaatggtc cccaggcctg ggcaaacatc gtcttcccag tggtcggaat
    22081 ttcagataca caattggtta atccgagcag ccggtttact gttaagggct ccgagcgcta
    22141 tagtttcgca tcggcaaaaa ctcaggggac ccgccgacga aggagcagaa gcatgccgga
    22201 ttcctgcgaa ccgactccac catccccgct ttctcagcag acagattccg gaaaagtcgg
    22261 catcagtcgt cgcggttttc tcaagggtgc cggactgacc gcggccggaa cagcccttct
    22321 cgaaggcgtc cagggtttcg ccactgaggc ccggggtgat tctgtccagg gtgtaaccga
    22381 gctgggcccg ggaccggccg ccatccgcct gcacgtcaac ggcacagagc acacggtgca
    22441 gattgagccg cgcaccacgc tggcagaggc tctgcgtatc cacctgggga tgaccggcac
    22501 caagattgta tgcgatcgcg gcgtctgttc cggttgcacg gtctggctcg acaagatgcc
    22561 cgtcaacagc tgcatgaccc tggccatcga tgcaatcggc catcacgtta ccaccattga
    22621 aggcctctcg accgaggaga aggttcatcc gcttcaggct gcgtttgtaa agcacgatgc
    22681 cctgcaatgc ggattctgca cgccgggtat ggtcatgagt tgtgcggccc tgctggagcg
    22741 caatccgcat ccctcggaac acgatgtgcg ccatgctgtc tccggaaatc tgtgccgctg
    22801 cggcacctac ccaaaagtct tcgccgccac tctggacgcg gccggcacct tgcgcaacgc
    22861 gtaacggagg acgtcccatg acgaaagaac ccatcgaaga cgcgatcacc gcgcaggtcg
    22921 cggctcccgc aaagaagaca gtacagatgc cggtcggcgt gcccggatac acactcgccg
    22981 aggaatcgcg cagcgtgcct gcgaccgaac ccccggtttg gccgaccaac aaagatctga
    23041 aatacgtcgg acagtccatt gaacgctacg acggccagct gaaagcctcc gggcgcgccc
    23101 gatacacatc ggatattcag ctgcccggca tgctctacgc gaagttcgtc aacgcgacgg
    23161 tgcctcacgc aaagatcgtc tcgatcgata ccgcggcggc ggaaaagtat ccgggggtga
    23221 agggtgttca cgtcatccag catgtgctcg gcaacgccga gcttcgcaac cccgcgctgg
    23281 aaggacaggt gaagtatccc gttgtgcgct atgcgggcca gccgatcgct gccgtcgcgg
    23341 ccaccacacc ttatgcggcg gaagaggcgg ctgcgctgat caaagtgaag taccaggcca
    23401 tgccgttcgt ggtcgacgac gtcgaggcac gtcacccaga cgctccgaat gtcttcccgg
    23461 gtgcagccga tgaggctggg agcgccggcg gcggtggcgg gcccaacgac gtaaagcaga
    23521 ccggcaacgt gcacggtccc tccgttcaca aacacggcga tacggaacaa ggcttcaagg
    23581 atgccgatgc cgttgtcgag gcccactatg tcacccaggt gcagacccac tccgctctcg
    23641 agacacatgg cgtcgtcgcc gactggaaac ccgacctgct gacggtgtac gcgtcgaccc
    23701 aaggcacctc cggcgttcgt gaggaactcg caggcttctt caaaattccg ctcacgaagg
    23761 tgcgtgtcat tacggagtac atgggtggcg gttttggcgc caaattcggc gccggcaatc
    23821 cgggtgtggt tgccgctgct ctttccaaaa agaccggggc tccggtaagg ctgatgctcg
    23881 atcggagcga ggagcatctc tccgtcggca atcgtcccag ctcggaccag gtgctgcgca
    23941 tcggcgcaaa gaaggacggc actctcaccg cgattcatct tctggcctac ggcactgcgg
    24001 gctgcggcac gggtgctggt tgtgcgggtc ccgcccagaa cctctaccag acggcgtctc
    24061 tgcacactga agagaacgat gttttcatca atgccggccc cggggcggcg atgcgcgcgc
    24121 ccggccatcc gcagggcgct tttgctctcg agcagaccat ggacgaactg gcggtgaagc
    24181 tcagcatgga tccgattgac ctgcgcgaca aggccgattc gcatccggtc cgaagagtag
    24241 agcgccagat tgtacgcgaa agcgatctct ggaagtcgcg taaccccgtc gcgggatcag
    24301 acaagggtcc ggtaaaacga ggcgtcggcc tggcgcagtc cgtctggtat cgcttcgtca
    24361 gcatgggctc aaatgcagag gtccgcattc ataaggatgg ctcccttgag gtgctgtccg
    24421 cagtgcagga catcggctcc ggcgtcagaa ctgtgatggc acagattctc gcggaacagt
    24481 tcggcgtgcc tccttcgcaa atctccgtca aggtcggcga taccacttat cccgagggtc
    24541 cgcagtccgg cgggagcgtc acgacgcttt cgctcacgcc ggcggtccgc gatgcagcct
    24601 ggcaggcttc acagaaattc ctggcggata tcgccccggc attcggaacc acggccgacg
    24661 atctcgtcct gatcgatggc gaagtgcgga gccagtcagg caaattcact ccgatcagct
    24721 tcaaacgcgc cgcctcaaag atgtcctcat cgcagatcgc ggtgcacgcg aagcgcgttc
    24781 cggactacga tccgaaaaaa tacctgactt acggcggcgt cgacgtcgcg gaagttgaag
    24841 tcgataccga actcggcagg atccacgtac ggcgcgtcct ctccgttcat gattgcggac
    24901 gaccgatgaa tcccgctcaa accatcagcc agatcaacgg cggcatcatt cagggaatct
    24961 cgttcacgct gttcgagaat cgactgctcg atccgaagta cggcctgatg gtcaaccccg
    25021 acctcgaaca gtacaagatt gccggatcgc gcgagatccc tgaaatcgat gtacgcctgg
    25081 ttgaggccta catcggccaa agttccacgg acgcctcggg aatcggtgaa gccgccggag
    25141 tggtttcgat cggagcggca attgcgaacg ccgtttacaa cgctaccgga gttcgcgtcc
    25201 gcagcactcc catgacgccg gccgtggtgc tggctgcgtt gcgcacgcag ggaaaggaga
    25261 tggcatgaac ggcttttcca tcgctgactg cacgacggtc aatgcagctt tgtcgcaatt
    25321 gaaggatggt gccgtcgtca aggcgggcgg agtcgatctt ctcgatcgca tgaaggacgg
    25381 catccagcag ccagaccggc tcgtgaacat ccgcaacgtt gcagaacttc gtggaatccg
    25441 cgaaaccgat caggggctca cgatcggtcc gctcgcaacg ctggccgaaa tcagcgacca
    25501 tcctcacatc cgtgagcact atcagatcct cgccgacgcc tgtggccacg cggccacacc
    25561 gcatatccgc aatatggcga ccgtcggtgg aaacctgctg cagcacgtgc agtgctggta
    25621 ctaccggtcg tcggattttc agtgcatccg gcacggtaag gacctctgtt tcgcattcga
    25681 aggcctgaac cagtaccacg ccatcatgga gtacagtacc tgcccatccg tcgcgccgtc
    25741 ctccgccgcg gtggcgctgc tcgggcttgg ggcgagtgtt gagctgactt cagctaaacg
    25801 tggcaagcgc accctcggca tacgggatct ctacatcgtc cccgatgcca atccgacgaa
    25861 attcaccgtg atcgagcccg atgagctgat caccgcgatt ctcattccga aaccagcgcc
    25921 cggcaccagg tccgcttacc agaagtatgg agagaaggaa agctttgact gggcgattgc
    25981 cgacgctggc gtggttctcg aaatggacgg cgacatctgc aaacgcgccg tgatcacgat
    26041 gggtgctgcc tctccggtag tgcggcgttc ttcgcaggcg gaagcggcgc tcgtgggaaa
    26101 accgattaac gaggaaaccg cgcgtgcagc aggcaaggtt agtgtcgaag gcgctacgcc
    26161 cctttcccag aatgcttaca aactcgacct cttcccggtt gccgtatacc gcacggttct
    26221 gctcgccgcc ggaaagatgg atcgcgatcc cagcgcggct gggtgacgga ggaaccatga
    26281 gcaccatcaa cgcgacccaa ccctgccaat gcctccgcag caagaatcct tacggatcga
    26341 cgcctctcaa tgccgagtcc tggctgccca gcttccatag tgtgacgacc tactggtgca
    26401 tgcgaaccat gggacccgca ggccccgacg atcatttcgt ccacctctcg cgctgcaagc
    26461 cgggccgccc ctgctatgag gagcgagaaa gttaagatct gctttccagt cctcgctggg
    26521 tcgctctgtg cctttcccgt gccggatcat tcgccgtgcc gctttccacc tttgctagtc
    26581 tgaaactatg ggaaattccc ccacagcgag catggtgacg ctgcatccgc gcggcattct
    26641 gttcgacatg gatggtgtgc tggtcagctc actcggttcg gtggagcgca gttgggagac
    26701 gtgggcccag gcacgtggaa tcgatcccga gttgactatc cgcacagccc acggccgccg
    26761 cgccattgaa acggtgcgcc tgcttcgccc cgatctcgac gagatgaagg agctggaatg
    26821 gctcgaagag atggaatgtg ccgataaggt cggccttgaa acgctcgccg gagtcgaacg
    26881 cattctcgag aatttgcccg acaaatactg gactgtcgtc acctccgcta ccgaacggct
    26941 ggcgcgcagc cgtatggagg caggcggaat tcgcgttccc aaagaaatca tctctgctga
    27001 tatcgtcacc cagggcaagc cccacccgga gccctaccgc aggggagccg agttgcttgg
    27061 ccttccccct tccgattgcc ttgtgatcga ggactcatcc tcaggcgcgc tcgcgggaca
    27121 cgaagctggc tgcaaagtgc tggccaccct ttactcgcac tcgttcgaaa gcctgcgaca
    27181 cgcggactgg atcgttccat cgctcgagga tgtgcatgtg caggtcctgg cagatcggct
    27241 cgaagtccgt ttcactccga tggagcgcag cgcagcaatc gcagcccgtt gatcgctcac
    27301 cgaatataat gaccgaaccg ccatggcacg ttctgttcgc gtggagtacg ccgaccagcg
    27361 caagatgcgc acccgcaaca cgcgccgcta tcgggcggcg cactggccca tctggatttg
    27421 ggtcttcttc ctcgcccccg gtccgttgac cttcaacttg tttgaccgcg gcggaaatcg
    27481 ctggaactgg atctggctgg cgcttgttct ggtgggcact ggcatcgccg cgttgttcgg
    27541 ccagttgccc ggtgccgagc ccgcgcctta catcctccgc ttcactgagg acagaccgaa
    27601 ccctctctac cgccgcattt gctacacctt cgcgtggaat gctcttctga atttcgctct
    27661 gctcaacctt gccggacttg cctgggccgt cgcgagcggc caatggcggt tgaagcagat
    27721 atacgctcac gggtattccc cgatcctgat tgccgttgtg cttcttgggg ccttcgggat
    27781 cctgccgcgc gttcgccgct ccactcaggg agagggttgg gagaggagat acttttatgg
    27841 cgcggtatgg tccgtaactg cggcgcagac cattctgctg atcctctgga aagctctgcc
    27901 gaagaatcac gcgaccgaca ttgcgaaact gctcctctac gcctctgtgc tggtattcgt
    27961 cggcgtactc gctgcctggg gtgttttgcc gcgcacgcgg ccgattctcc ccggcgaact
    28021 catggtcgcg gactaaacag cccttacgga atcgttacaa agtcctttcg ccacgtttgc
    28081 agccagtcgt ctgcaatgga tgcagaggac gtgcgtcagt ggatctcaac cagtttgcac
    28141 tttacggact agccgctgtc atcgcagcag gcattggttt cttcttgtgg gtgctgctgc
    28201 gcctggagtt ggcgaagcga cagcacaaca atggacaggc gcacccagcg ggctctcgcc
    28261 ggatgcacca ttagtcctgt ttgcagttac aggttgccac gcgaagcctg ctcgcgctcg
    28321 atggcttcaa acaacgcttt gaagttgccc ttcccaaagc tgcggcttcc tttgcgctgg
    28381 ataatctcaa agaacagcgt ggggcgatcc tcgacgggct tggtgaagat ttgcagcata
    28441 tagccttcgt cgtcgcgatc caccaggact ccgagctttt ccagttccgt aatcggctcg
    28501 tcgatcttgc ccacgcgtcc ttccagctcg ctgtaatagc tatgaggcac ggtgaggaac
    28561 tgcactccct gcgcctgcag cttcgccacg gtctcgagaa tgtcgctcgt tgccagcgcg
    28621 atgtgctgca ctccaggtcc gtggtaatac tccaggtatt cctcgatttg cgatttcttc
    28681 cgcccttcgg ccggctcgtt gatggggaac ttcacacgtc cattcccgtt ggccatcacc
    28741 ttcgacatga gcgccgaata ttccgttgaa atgtccttgt cgtcgaagtg ctggtaaagc
    28801 gagaagccca tgatctgtgc gtagaagtca acccatcgat tcatctcgtt ccagccgacg
    28861 ttgcccacca tgtggtcgat gtgcagcagg cccactggcc gcgccagcgg atcctcgggc
    28921 atcgcgcgat agccggggag aaaggccccc ctgtagtcgc tgcgctcgac aaacgagtgg
    28981 accgtgtcgc cgtacgctgc aatcgtcgag atcctcacca tgccgttgtc gtctttcagc
    29041 tcaaacggct cgcgtacgct gcgtgccccg cgcttggtcg tctccttcca tgcctgccgc
    29101 gcgtcgtcta cccaaagcgc cacatcgtga accccgtcgc cgtgcacacg cacgtgttcg
    29161 gcgatatcgc cgtcggggcg aagaggcgtc gtcagcacaa accgaacctt gccctgctcc
    29221 agaacatacg aagcgcgatc ccggattccg gtttccgggc ccgcgtaagc caccagcttc
    29281 atgccaaaag cgagtcggta aaaataggcg gcctggcgcg cattgcccac ccaaaactcc
    29341 acgtgatcag tcccgttgag cggcagaaaa tccttttgtg cagcgacttc cgattgcaac
    29401 gctgttgcca tagcaaacaa ctcctgggga ttacggtacc cgtctccgaa ggtgcgcttc
    29461 agagcacacg gcgagcacta cgatataccc ccgtgaagtc acttggccag tgacgaaaaa
    29521 agaccaacgg cagggcaacc gacggagcaa aagcggcaac gttgggtctg gtcggtttca
    29581 tggcgcgcgg tgttcgatcg ccaacgtagt aacctgaacg cgcacgggcc gtctcaggtc
    29641 acaaaccgct gcagattgct ggaggcagcc ctctgcattc accccgaatc cgactttttt
    29701 agatgcccag gaaagatcac aaacccgcaa agaaacccgc caggcagaaa gaaacctctg
    29761 caggcctggt cgacgagcag ttagcacgtt accgcgccat gcgcgacttt gccatgactg
    29821 ccgaaccgag tggggcctcc ggcaacagaa caaaaaagaa gcttgaggag ggtctcccct
    29881 tcgtcgtcca gaagcatgca gccacgcgcc tccactatga cttccgcctc ggatggaagg
    29941 gcgttctcaa gagctgggcc gtcaccaaag ggcccagcta cgttgtcgcc gacaagcggc
    30001 tcgctgtcga agtcgaagat caccccatgg agtacggcgg ctttgagggc acgattccta
    30061 aaggtcaata tggcggcggc accgtgatgg tgtgggacag cggcacctgg gaacccgtca
    30121 ccgatgtcga cgaaggcctt cagaaggggc aattgaagtt catcctgcat ggaaagaagc
    30181 tcaaaggtca ctgggctctg attcgaatca aaaacaacaa attcggagat aaaggcaaaa
    30241 acaactggct cctcattaag gagcacgacg aatacgagcg gtcagccggc gaccccgcaa
    30301 tcaccgacga ggcgcccgac agcgccgtta ccggccgcag cctcgagcag attgccgacg
    30361 ctgaagatca cgtctggaat tcgcatgtcc cggaaaagaa ggctcctgtc aatcgctctc
    30421 ggctgacacg gcgccccgag agttcgacga cagtcgagca gcctgtgccc gggctggtga
    30481 aaaaagcacc cgtccccgat cgcaagtccg ttctgaaaga cgcaccggca gagaagatgc
    30541 cccgctttct gtcgccggaa ctcgcctctt ccgtggacca gcctcccgag ggcgacgact
    30601 ggctgcacga actgaaactc gatggctatc gcatccagat ccatctcgat gaaaaggacg
    30661 gcaagcgccg ggcgcgtctc ttcacccgca agggcctcga ctggacgcat cgcatgcccg
    30721 acgtggccag gaccgcaacc cagttgccgg tgcacgctgc cgtcatcgac ggcgaggtcg
    30781 ttgtagtgaa tgagaaaggc ctcaccagct ttgccgatct gcaggcagcg tttgatgaag
    30841 gtgcgccgca tcccctcacg tattacgcct tcgatctgtt gcatcttgac ggacacaacc
    30901 tgcgcaatct gcccctggag cgtcggaaaa ccatcctcga agggctgatc cagcagcacg
    30961 gcgaggatgg aatccgctac agcagccatc ttcgagccaa tgggggcgag atgctccgcc
    31021 atgcatgcca gatgggtgcc gaaggcatcg tctccaaact ggcaacatcc acctggcgtg
    31081 gagaaagatc gcgtctttgg cttaagtcca aatgcatact gcaacaggaa tttgtggtgg
    31141 gaggatttgc gccgcttgcc accggagcct acggcatcgg ctcgctcttg ctcggctatt
    31201 acgaagatgg tgagttgatt tatgcagggc gcaccggcac cggtttcaat caaaaaacac
    31261 acaagatgct gcgcgaccgc ctcgaggaac tgcgtcagcc gaaacccgca tataagagcc
    31321 ttccaacaga ggcgaagaaa gatgcagtct gggtaaagcc cacgatggtg atcgaggctc
    31381 ggttttcgaa ttggaccagc gatcacctcg tccgccaggc agcatttctc ggcctgcgcg
    31441 aggataagga tccgaaggaa gtggtgcgtg aagttgcgga gtcaattccg aggttgtcgc
    31501 gcaagcatac gaacagcgaa accgctcaac cttccacacc gcatcacgat gcaaaaaagg
    31561 cgaaacactc tgtgcctgca ggaactgcgg cgaaagttga gagccccaga ccctccaaat
    31621 cacccaaccg tacccaggtg gccgggctat ccatcactca tcccgataag attcttg
//
LOCUS       DASZYN010000029        13989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_194416, whole genome shotgun sequence.
ACCESSION   DASZYN010000029 DASZYN010000000
VERSION     DASZYN010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 13989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13989
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194416"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1625)
                     /locus_tag="VHE33_00835"
     CDS             complement(<1..1625)
                     /locus_tag="VHE33_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HVT96019.1"
                     /translation="MNLRCAAVLAWSCSLLFTAPVSPQTKRALLIGINTYQPVGTSAT
                     HPPGCTYGRCELGYFENLEGSVNDAQSMADLLTSPKFAFPANQVVLLANPSPPHPAPG
                     LILLPAGQTTRDGILAAMQKYLVDVPERGDTVVFYAASHGSLRVNSKGTKLTVLVNGH
                     YIHADSTLVPSDAYKGGYDVRDREMTRIFNAALDKGVHLTVIFDNCHSGGVTRGIPAK
                     YRERDLPFDPRDIAEAPELLPSGQPRPSPSQRTDNPALVLSAVQQDQTAKEMPDTVPA
                     NESHGAFTAALLEAMQVLPADAPAEAVYRRVRAVLGNADVPDEDPDLDATAERREQPL
                     FGGAVSESGALHAAVLATAADGKIWLDVGRVAGIGAGSEFTEIGNGQNIRLRIVASDG
                     IARSIATIVTPAGATVKTGDILELARWVPAQSAPLRVWHWPTLSRADVVAAITQIRAA
                     GVTSVSDPAEEQWTHLLSWNGNSWTLQQAGADSSIKLGPILNSDTLRQKVPSRAKLWI
                     NLPLSHEVAAQLLPPDPTSSVQSSPNLAGAHYV"
     gene            complement(2037..3788)
                     /locus_tag="VHE33_00840"
     CDS             complement(2037..3788)
                     /locus_tag="VHE33_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HVT96020.1"
                     /translation="MNTTFRGFFLFLVAAVLSPPLVAQRASDSFETNPGWWKDAVIYE
                     IYPRSFQDSNGDGVGDLNGITSRLDYLQALGVNAVWLSPVYPSPQVDFGYDISDYTTI
                     DPKYGTLSDFDRLVAEARKRGIGIIMDLVLNHTSDKHPWFKESAASRTNPKADWYVWR
                     DPKMVEGHREPPNNWTSVFGHSAWQWDPARRQYYYHRFYMQQPDLNWNNPDVRKAMYD
                     VERFWIQRGVAGFRLDAITSLFEDPSFSDEDYVRGPDGKALINAYGDKEVNTDKTDDL
                     PKVNDVLRELRQVEDETHNRKVILIGETYLRSIEDLRRMYGAHNDELDLPMDMQIGFI
                     NQFDIGLFRKRIDEAETAIGGNQPLFVFDNHDNPRWDRYITSDHKDDVGRVIATILLA
                     SHDTAMMYYGDEIGMVTTPPTRKDDVKDPIGVTGWPEEKGRDGERTPMQWNDGPNAGF
                     APGGTKTWLPIPPSYEQINVTTEEHDPDSMLHWYQKIIGLKRTDSALHDGEEIMLNTS
                     DEHVLSWLRKSKSGEAVVVACNFSDQPQKASFDLSSQNIRGTGVTTLLKTPGAADPVS
                     LKSVQLGPYGVYIGRVQ"
     gene            4326..8009
                     /locus_tag="VHE33_00845"
     CDS             4326..8009
                     /locus_tag="VHE33_00845"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT96021.1"
                     /translation="MRKYSGLAAGCALFAALCFCTTLQAQAVYGSINGTVTDNTGAVV
                     PNATITVTDVAKGTSVTTTSNASGEYTVQHLIPDTYDVKVEAQGFQTFSATGVQVLAD
                     TSPKIDAAMKVGAASQTVEVSASTIPELKTDRADVSTEFTTREVQDLPIPDRNFTNMQ
                     LLLPGAQPLGWGHASDENPQGSKQIQVDGQAFGGVAFELDGTDNQDPILGIIVINPPL
                     DALSETKITTQNFDAEFGKAVSSFVTAQTKSGSNQFHGSAFDYRESNANLATDPYSQY
                     LSATTTKSVPAGLKNQFGGSIGGPVLKDRLFFFGDYQGVRQKVGIADSNITVPTAHLI
                     STCLGQSTASNGVAGCDFSEYAAAEGIPNSTTPYLLYDPSTGAPYATNVIPAAELSQP
                     ALNLLKLLQPYAKGTTSATKNNYSANGTGLFNSNQWDVRVDDQVASNIHAFARFSRFT
                     SLLSGSTVFGDAGGPGFGIQGYGGKSNGADDSLASGMDIAVSPKLLTDFRLAYYRYGA
                     VTSKYDQGTSFATQLGIPGMNMGDSFTSGAPAFNIADVDVHGNNGPNNPQSQGSQYGS
                     GLNINRCNCPLTQKEDQFQIVNNWTKIYGNHSLKFGVDLRYARNLRVPSDNDRTGILS
                     FGSGPTSNNGATSTGLGFASFVLGDVTQFQRYVSVSTNAKEFQKRFFFYAQDTWHARP
                     NLTVNIGTRYEFYFPESVNGKGNGSLMPLNGGSGGYQTDGYFHVAGYGNVPTNMGWSS
                     TTNTWNPRIGVAWQPDSKTVVRSGYGRSFDLGVFGSIFGHVVTQNLPVLANQNVNAPT
                     TTTPAFTLAAGPPKNVFPSVPSSGLLPVQGYAISPKARPDSLRLPTLDAWNLSVQRSI
                     TPTLSVTVAYVGNKGTHTLSDGDGNNTNPNEAAINLPAQYSVTGQPLHYDSTVPTGTV
                     QPGGPAGIPGIAADGGTAVSNYLSRFYGGGLTACTDPNYATPVEPGITRGMCGWTNGI
                     NYYGDDQDTHFNALQVSLAQQMSRGLSFNVQYAWQRGIDYANGYATWDKRPERGRDGS
                     IREQQFVVYGIYEVPFGKGRRWATGVPRWEDEVIGGWEFSPVLNWSSGLPFTLGVGGG
                     CGSTLPGSAPCYPNGSGKQVHTHLTKFDPVSHSRTYFTKADVPSSKFPAAGLDKIGTM
                     GRNSVYGPHYFSGDLSLQKNFPIYESVLAQFRIDAYNGFNHINPGNPGTTQTDSNAVI
                     NSEPALNVYTNPRQLQFSLRVQF"
     gene            8084..9112
                     /locus_tag="VHE33_00850"
     CDS             8084..9112
                     /locus_tag="VHE33_00850"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT96022.1"
                     /translation="MPGLLLLCVLALASLTAYAKAQNDEGTRQPSVADVRALLAQGKL
                     NDASRGLDALASLQPEPPGVERLRGIVEYESNHLAEADAAFARALAQDPTDLDAMQMR
                     GVVLYRRGKPQAAIPLLQRAHSSGSIANADPNYVLGLCYIDAKRYDDARRAFATQYDF
                     APESAAAWLVTARILFRQELTSQATIAVQKALQLDSRIPLAHRLMGEIDLANGDVNGA
                     LNQLHQELKVNPLDGETYNRMGDALIRGGQFARAQQVLDRAVLLEPNASGPYILLGRA
                     LLEEHDPVTATMYLQRAEKMDPSNTMIHMLLGRAFTVVGRKEDASREFEALQKLQRTT
                     TPPPSSNP"
     gene            9212..10873
                     /locus_tag="VHE33_00855"
     CDS             9212..10873
                     /locus_tag="VHE33_00855"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT96023.1"
                     /translation="MQEASQAFRAGSEAFRRGDLTEADKQFSQAVRLAPGIEEGHSAL
                     GVVLYAQGHYPQAIAELRTALKLKPGDRNAEESLAQAYSQTNAMESAIAIFENMDREA
                     PLRAELLAMWARDLAALANPLTAIDVMARAVAADPQNAELVDQLGSLDAQAGRWQNAE
                     TAFHKALTIDSGLASAHMHLAVVLSHEQRTGEAEIEFARASQIDPGTATVQFEWGNAL
                     VAANQDDQAIPHFQKAMALEPNQIDAEYQLALVLQRAGKEEDAVPLLQKVVASRPQQA
                     EPLTNLGLALVQTGHAKEAIPLYQRAAILSPENPTVHEDLGAAYLQENNIDDAIGELQ
                     TGLRIAPQDPHLHYDLGLAYKLKDDLPHAIVELEAALRLEPDSPDAPYTLGILYMQDG
                     HFDEAVQNLQTALKARPQNGDGWSILGSIYRQQGKYDEAANALRRAIELVPEQPGPHI
                     TLASVLQDEGKQEDAAAERKKAAELTRVAVNRQRAMFATNAGNAFLDKGATADAIVHY
                     QEAISSDPSYAEAHRQLAAALLQQGRMAEAAAERQKAEALTAKNP"
     gene            10895..12766
                     /locus_tag="VHE33_00860"
     CDS             10895..12766
                     /locus_tag="VHE33_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HVT96024.1"
                     /translation="MNRRKFLTSLSRSALVLPFADVLQLAKPLWWNKALMAGQQASSP
                     RPGAQQSYDAKPMPPPPGPTSPVAGTPLGVQFVDVAIQSGLHAKTIFGGEGTNKYLLE
                     TTGCGVAFYDYDQDGWLDIFLVNGWRLEGFPKGNEPTCRLFKNNRDGTFTDVTAKAGV
                     GHSGWGQACCIGDYDNDGWDDLFITYYGQNVLFRNNGNGTFTDVTAKAGLLQPKMRWN
                     SGCAFLDYDRDGHLDLFVGNYIDFDLKTAPLPESGPCLYKGILVACGPPGLSPGKNIL
                     YHNNGDGTFLDVSEKAGMGTTLGNYALSAAVADFDNDGWPDIYVANDSTAATLYQNQK
                     DGTFRDVAIEDGVAFSPDGRPQAGMGVTVGDYNRDGWLDIAKTNFAGDTDSLYAGFGG
                     GNFEDRTYQAGLGVNTRYLGWGIGFVDIDNDGWLDLFVANGHVYPEVSSVKTEAPYAE
                     HKYLYRNLRNGRFEDASALGGTGITAAASARGCAFGDYDNDGDLDVVVNCVNSIPQLL
                     RCDSGGKRHWVKVKLVGTKSNRTGIGGRVRVTAHPQTDQSMVFIDEVRSGGSYFSQSD
                     LRLHFGLDQAESIDVLEVHWPSGQVDTFRGLPVDRLYVIEEGAKEPRGFLLPSMRKV"
     gene            12768..13901
                     /locus_tag="VHE33_00865"
     CDS             12768..13901
                     /locus_tag="VHE33_00865"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HVT96025.1"
                     /translation="MTLRSRTANLLAAFLCAGFLSGTASSGDTESWQIGPFTRPIVGN
                     PVIKPNPAALFTDPIRKTAVRWESLHTFNPAAVVRDGMINVLYRAEDDTGAMQIGMHT
                     SRLGRAQSVDGTHFTRSETPVFYPEADSQQAREWPGGVEDPRIVEAEDGTYVLTYTQW
                     NRITYSVGIATSPDLEHWIKHGPAFFNAAGGKYINLKYKSAGIVTALRGGRLIAVRID
                     GKYWMYWGEGAIHLATSSDLIHWSPVEGADSEPLEVLEPRRGRFDSSFPETGPPPILT
                     SRGIVMIYNGKNAATGGDPQLGANTYAVGEALFDARDPARLLARTDSPVLEPVLPYER
                     TGQYSAGTTFAEGLVYFHNQWFLYYGCADSLVAVATAPANVTK"
BASE COUNT         3019 a         3793 c         4264 g         2913 t
ORIGIN      
        1 aacacgtagt gcgcaccggc gagattgggg ctcgactgca cggaactggt tggatcggga
       61 ggcaggagtt gcgcggcgac ttcgtgcgac agaggaagat tgatccagag ctttgctctg
      121 gatggaactt tttgtctgag agtatcggaa ttcaggattg ggccgagttt tatcgaggag
      181 tctgcgcctg cctgttggag ggtccagcta ttgccattcc agctcagcag gtgggtccat
      241 tgttcttctg cggggtcgga aacgctggtc acgccggcgg cgcgaatctg tgtaatcgcc
      301 gcgacaacat cggcgcgcga taaggtgggc caatgccaca cgcgcaacgg cgccgattgg
      361 gccggcaccc atctggccag ctccaggatg tcgccggtct tcacggtcgc gcccgctgga
      421 gtgacaatcg tggcgatgga acgcgcgatt ccatcggaag ccacgatgcg aagccggatg
      481 ttctgaccat tgccgatttc agtgaattcg ctgccagcgc caatgccggc gacgcggccc
      541 acgtcgagcc agatctttcc atccgcggcg gtcgcgagca cagccgcgtg cagagcgccc
      601 gattccgaga ccgctccgcc gaacaaaggt tgctcacgac gttctgcggt ggcgtcgagg
      661 tccgggtctt cgtcaggaac gtctgcattg cccagtaccg cacgcacgcg ccggtaaacg
      721 gcctcggcgg gagcatcggc tggcagtacc tgcatggctt cgagaagtgc cgcggtgaag
      781 gcaccgtgcg attcgttggc agggacggtg tccggcatct cctttgcggt ttgatcctgc
      841 tgcaccgcag agaggaccaa ggcagggtta tctgtccgct gcgaagggct gggacgcggc
      901 tggccgctgg ggagcaattc gggtgcttca gcaatgtcgc gcggatcgaa gggtagatcg
      961 cgctcacggt attttgcggg gatgccccgc gtcacgccgc cactatggca attgtcgaag
     1021 atgaccgtga ggtgaacgcc cttatcgagt gcggcattga agatgcgggt catctcgcga
     1081 tcgcgcacat catagccgcc cttgtatgcg tctgagggca ccagggtgct gtcggcgtga
     1141 atgtaatgac cattcacgag gacggtcagc ttggttcctt tgctgttgac gcgcagcgat
     1201 ccatgactgg cggcataaaa gacaacggtg tcgccgcgtt caggcacatc gaccaggtac
     1261 ttctgcatgg ctgcgaggat gccgtcgcgg gtggtttgcc ctgcgggcag caggatgagg
     1321 ccgggtgctg ggtggggcgg gctgggattg gcgagcagta cgacctgatt cgccggaaat
     1381 gcaaattttg gactggtgag caggtcggcc atggactgcg cgtcgttaac cgagccttcg
     1441 aggttttcaa aatagccgag ttcgcagcgt ccataagtgc agccgggcgg atgggtcgcg
     1501 ctggtgccaa cgggctggta ggtgttgatc ccaatgagca aggcgcgttt ggtctgcggc
     1561 gaaaccggag ccgtgaaaag gagggagcaa gaccaggcaa gcacggcagc acaacgaagg
     1621 ttcatacgag atcctcgata cgaactgccg ggggaaaact ttcatcgagt atagctctct
     1681 gggcggcagg gcagcgtcgc aggatggagg aaaccaattc agcggatccc ggcagttgcg
     1741 ccagaaatgg ggtgatacgt gtgaggtggg ccacccagga gactcttgaa gagagctgtt
     1801 tggagcgcgc ctttctgttg aatctatccg cgcacggcgg cagtagccag accgctaaat
     1861 cagagaaacc aaccgttgac caatctcacg acgtgggatg gagatctcgg cgctgaccag
     1921 aacggaatcg gaacgctgac ggcacatttc ccgacgacca acaagacgtc aactctgcgg
     1981 tgatagcttc cgcaattgtt aaagcattca gttagctacc atattgttcg gaatgcttac
     2041 tgaacccttc caatgtagac tccataagga cccagctgca ccgatttgag agacaccggg
     2101 tcggcagcgc ctggcgtttt caacagcgtc gtcactccag tgccccggat gttttgagaa
     2161 gacagatcga agctggcttt ctgcggctga tcggagaagt tacaggccac gacgactgct
     2221 tcaccgctct tcgacttgcg cagccaggat agcacgtgct cgtcgctggt gttcagcata
     2281 atttcctcgc cgtcgtgcag agccgaatcc gttctcttca ggccgatgat tttttggtac
     2341 cagtgaagca tcgaatccgg gtcgtgctct tccgttgtga cattgatttg ttcgtaactc
     2401 ggaggaatgg gtaaccaggt cttcgtcccg ccaggtgcga agccggcatt ggggccgtcg
     2461 ttccattgca ttggagtgcg ctcaccgtcg cggcccttct cctcaggcca tccagtgacg
     2521 ccaatggggt ctttgacatc gtctttccgg gtcggtggcg tagtgaccat gccgatctcg
     2581 tcgccgtagt acatcatcgc ggtatcgtgc gaggccaaaa ggattgtggc gatgactcga
     2641 cccacatcgt ccttatggtc agatgtgatg tagcgatccc agcggggatt gtcgtgattg
     2701 tcaaaaacga acaatggctg gttcccgcca atcgctgttt ccgcctcgtc gatgcgttta
     2761 cgaaagaggc cgatatcgaa ctggttgatg aacccaatct gcatatccat gggcaaatcg
     2821 agctcgtcat tgtgcgcgcc gtacatcctg cgcaggtcct caatcgagcg caggtatgtt
     2881 tcgccgatga gaatgacctt tctgttgtgg gtttcgtctt cgacctggcg cagttcacgc
     2941 agtacgtcat tgactttggg aagatcgtca gtcttgtcgg tattgacttc cttgtcgccg
     3001 taggcattga tgagcgcttt gccatcgggt ccccgcacat agtcttcatc gctgaaggaa
     3061 gggtcctcga acagggacgt aatagcatcg aggcggaagc ctgcgacgcc gcgctgaatc
     3121 cagaagcgtt cgacatcgta catggcttta cgaacatcgg gattgttcca gttgaggtca
     3181 ggctgctgca tgtagaagcg atggtagtaa tactgcctgc gcgcgggatc ccattgccat
     3241 gcggagtggc cgaagacaga ggtccagtta ttgggaggtt cgcgatggcc ctcaaccatt
     3301 ttggggtcgc gccacacata ccagtcagct ttgggattgg tgcgggacgc tgcagattct
     3361 ttgaaccagg gatgcttgtc ggatgtgtga ttgagaacaa gatccatgat gattccgatc
     3421 ccacgcttgc gtgcttccgc gacgagtcga tcgaagtcgc ttaatgttcc atacttcgga
     3481 tcgatcgtgg tgtagtcgga aatgtcgtag ccaaaatcga cttgcgggga aggatagaca
     3541 ggcgacaacc aaaccgcatt tacgccgagt gcctgcagat agtcgagtcg tgacgtgatg
     3601 ccgttgagat cgccgacgcc gtctccattt gaatcctgga agctgcgtgg ataaatctca
     3661 tagatgacgg cgtccttcca ccagccagga ttcgtctcaa acgaatccga tgcgcgctgg
     3721 gccacgagag gagggctgag aacggcagcc accaggaaca ggaagaatcc acggaacgtg
     3781 gtgttcatcg gggggtctcc gatcgccaag caactgcctc tgagctgctt gcagcacacg
     3841 agagacggcg atccagggcc gaatgctacc acattgaacg cgatgatgaa tctgccgcac
     3901 ttgcgcacgt agttctggtg tgatggtgcg gatcatttca tttcatccca acgcagcgca
     3961 acgggaagag gttagtcgcg gtctgacctc tcgcagagaa ctactaatat gggtttgcag
     4021 gcgcgagtag cttgacaacg gtcattgcaa ttgcctaaag tcactgccgc agtcaggaat
     4081 gtcgctaact cgtgcggctt tctccccagt tcgcgagcgg acgaacgcta accgcaagcc
     4141 gcgattgaaa acgccaacgt acgttaaaac cgccagacgc aggtcggaaa ttcaaaagcg
     4201 gccaaactca tttacgaggc cgcatttgca ttcaaaaaaa acaaaatcac gaccgggccg
     4261 gatcgctctt tacagaaggt ttgagacagg aacggaaatt cctgggaact cttgtggagg
     4321 caattatgag gaagtattca gggctcgcgg cagggtgcgc tctttttgcg gcgctgtgtt
     4381 tctgcactac gctgcaggcg caggcggtgt acgggtcgat taatggaacg gtcaccgata
     4441 ataccggtgc tgtcgtgccc aatgcgacca tcactgttac cgatgtcgca aagggaacgt
     4501 ctgttacaac gacttcgaac gcctcgggcg aatacaccgt ccagcacctc attcctgata
     4561 cgtacgacgt aaaagtggag gcgcagggat tccagacctt cagcgccacc ggcgtccagg
     4621 tattggctga tacctctccg aagatcgatg ccgcgatgaa ggtgggtgcc gcctcgcaaa
     4681 ccgtggaagt gagcgcgagc accatcccgg aattgaagac ggaccgcgca gatgtgtcca
     4741 ctgaattcac aaccagggag gttcaggacc ttcccattcc ggatcgcaat ttcaccaaca
     4801 tgcagttgct gctgccgggg gcgcagccgc tcggctgggg ccacgcgtcg gacgagaatc
     4861 cgcagggcag caagcaaatc caggtcgacg gtcaagcgtt tggcggggtt gctttcgaac
     4921 tcgatggcac ggacaatcag gatccgattc tcggaataat cgtgatcaat ccgccgctgg
     4981 atgccctctc cgaaacgaag atcaccacgc agaatttcga tgcggagttt ggaaaggctg
     5041 tttcgtcgtt cgtgacggcg caaaccaaat cgggatcgaa tcagttccac ggcagcgcgt
     5101 tcgactatcg cgagagcaac gcgaatctgg ccacggatcc gtattcacaa tatttatcag
     5161 caacgacgac gaagagtgtt ccggcgggct tgaagaacca gttcggcggt tcaattgggg
     5221 gtccggttct caaggatcgc ctgttctttt ttggtgacta tcagggcgtg cggcagaagg
     5281 tgggtatcgc cgacagcaac attacggtcc ccacggcgca cctgatctcg acctgtctcg
     5341 gtcagtcaac agcctccaat ggcgtcgccg ggtgcgactt cagtgagtac gcggcagcgg
     5401 aaggcatacc gaattcgacg actccgtatc tgctctacga tccctcaacg ggcgcaccgt
     5461 acgcgaccaa tgtgattccg gcagctgagc tgtcgcagcc agccctgaac cttcttaagc
     5521 tgcttcagcc ttacgcgaaa ggcacaacat cggctacgaa gaataactac tcggcgaacg
     5581 gcacgggcct cttcaacagt aaccagtggg acgtacgagt ggacgaccag gtagcgtcga
     5641 acatacacgc cttcgcgcgc ttcagccgat tcaccagtct gctgagcggt tcgacggtct
     5701 tcggcgatgc gggcggaccc ggcttcggca ttcagggcta cggtggcaag tccaacggcg
     5761 ccgacgacag tctggcttcc ggcatggata tcgccgtgtc tccaaaattg cttacggact
     5821 tccgtctggc ctattaccgt tacggcgcgg tcacgtcgaa gtacgatcag ggtacgagtt
     5881 ttgcaaccca gcttggaatt ccggggatga acatgggaga cagcttcacg agtggagctc
     5941 ccgcgttcaa cattgccgac gtggatgtcc atggcaacaa cggcccaaac aatccacaga
     6001 gtcagggttc gcagtatggg agcggtctca atatcaaccg ctgcaactgc ccacttacgc
     6061 agaaggaaga tcagtttcag atcgtcaaca actggactaa gatttacggc aatcactccc
     6121 ttaagttcgg agtggacctg cgctatgcac gcaaccttcg cgttccatcg gacaacgacc
     6181 ggaccggtat cctgagtttt ggttccgggc ccacgtccaa caacggggct accagcactg
     6241 gccttggctt tgccagtttt gtgcttggag atgtaactca gttccagcgt tacgtgagcg
     6301 tttcgacgaa cgccaaggaa ttccagaaga ggttcttctt ttacgctcag gatacttggc
     6361 atgcgaggcc gaatctcact gtcaacattg gtactcgcta tgagttctat ttccctgaat
     6421 cggtaaacgg taaggggaat ggatcgctga tgccgctgaa tgggggaagc ggcgggtatc
     6481 aaacagatgg ctattttcat gtagctggtt acggcaacgt tcccaccaac atgggctgga
     6541 gctcaacgac gaacacctgg aacccgagga ttggcgttgc ctggcaaccc gattccaaaa
     6601 ccgtcgtccg gtccggttac gggcgtagct tcgatttggg tgtcttcgga tcgatctttg
     6661 gacacgttgt gacgcagaat ctgcctgtgc tggccaacca aaatgtcaat gcgccgacga
     6721 caacgactcc ggcatttacg ctggcggcgg gtccaccgaa gaatgtattt ccgtctgttc
     6781 cttcgagtgg gttgttgcct gtccagggtt atgcaatcag tccgaaggcg cgtcctgaca
     6841 gcttgcggct gcccacgctg gacgcctgga atctcagcgt tcaacgatcc atcacgccga
     6901 cactttccgt gactgtagct tacgtgggca acaagggcac acatactctg agcgacggcg
     6961 acggcaataa caccaatccc aatgaggcgg cgatcaatct gccggcgcaa tatagtgtta
     7021 cggggcagcc gcttcactac gattccaccg ttcccacggg aacggttcag cctggtggcc
     7081 ctgcgggaat cccgggcatc gccgccgacg gcggcactgc cgtctccaac tatttgtcac
     7141 gcttttacgg tggcggcctc acagcatgca cagatccgaa ctatgccacg ccggtcgagc
     7201 ctggtatcac acggggaatg tgcggatgga ccaacggcat caactactat ggcgacgacc
     7261 aggacaccca cttcaatgcc ctgcaggtct cgctggccca acagatgagc cgtggccttt
     7321 catttaatgt tcaatatgcc tggcagcggg gaatcgatta cgccaacggt tacgctacct
     7381 gggacaagcg ccctgaacgc ggaagggacg gcagcatccg cgagcagcag ttcgtggtct
     7441 atggcattta cgaggtgccc ttcgggaagg gacgcaggtg ggcgaccggt gtgccgcgct
     7501 gggaggacga agtcatcggg ggctgggagt ttagtccagt gctgaactgg tcgagcgggc
     7561 tgccgtttac gctgggtgtg ggtggcgggt gtggcagtac actgccgggc agcgcgccgt
     7621 gctatcccaa tggctcaggt aagcaggtgc atacacacct gaccaaattc gacccggttt
     7681 ctcacagcag aacctacttc accaaggccg acgtgcccag ttcgaaattt ccggccgccg
     7741 ggctcgacaa gatcggtacc atgggccgga acagtgtgta cgggccgcat tacttcagtg
     7801 gggatctttc tcttcagaag aatttcccga tctatgagag tgtgctggcg cagttccgca
     7861 ttgatgctta caacggcttt aaccacatta accccggtaa ccccggtacc acacagacgg
     7921 actcaaacgc agtcatcaac agtgagccgg ctctcaacgt ttacaccaac cctcggcagc
     7981 tgcagttctc gctgcgcgtg cagttctagg caacgtgtcc ccggtcccgg aggggagtgc
     8041 tcactcccct ccggatgctt ttgaggagta aacttcccgc atcatgcctg ggcttcttct
     8101 gttgtgtgta cttgcactgg ccagtctgac ggcatatgcg aaggctcaga acgatgaagg
     8161 aacaaggcag ccttccgtcg ccgatgtacg cgcgctgctg gcgcagggca agctgaatga
     8221 tgcgagccgc gggctggacg cgttggcgag cctgcagccg gagccgcccg gcgtagagcg
     8281 gttgcgcggc atcgtcgaat atgaaagcaa ccacctggcg gaggctgatg ctgcgtttgc
     8341 gcgggcgctg gcgcaggacc cgacggatct ggatgcgatg cagatgaggg gggtcgtgct
     8401 ctatcgcaga gggaagccac aagctgcaat tccattgctg cagagagcgc actcgtcggg
     8461 atcgatcgcg aacgcagatc cgaactacgt gcttggcctg tgctatatcg acgcgaagcg
     8521 atacgacgat gcgcgacgcg ccttcgcgac acagtatgat tttgcaccgg aatcggccgc
     8581 agcatggctg gtgacggcac gcatcctgtt tcggcaggag cttacgtctc aggccacaat
     8641 tgcggtgcag aaagcgctgc aactcgattc gcggatacca cttgcgcatc gattgatggg
     8701 cgagatcgat ttggcgaacg gcgatgtaaa cggcgctctg aatcaattgc atcaggagct
     8761 aaaggtgaat cctctggatg gagagaccta taatcgcatg ggcgatgcgt tgattcgcgg
     8821 tgggcagttt gcgcgtgcgc aacaggttct ggatcgtgcg gttctgctcg agccgaacgc
     8881 cagcggacca tacatcctgc tgggcagggc gctgctggag gagcacgatc cagtgaccgc
     8941 aacgatgtat ctccagcgcg cggagaagat ggatccgtcg aacaccatga tccacatgtt
     9001 gcttggacgg gcgtttaccg tggtcgggcg caaggaggat gcaagccggg aatttgaggc
     9061 gctgcaaaaa ttgcagagga ccaccacgcc gccgccgtcg tcgaatccct agcgcggacc
     9121 atggagggtg ttcttgagat ttgtgtggaa gtcagtgttc gcggctggtc tgggggccgg
     9181 gacgctgttg tgcggagcgc aatcgacagc gttgcaggag gccagccagg cctttcgagc
     9241 cggaagcgag gctttccgca gaggcgattt gactgaggcg gacaagcagt tcagccaggc
     9301 tgtacggctg gcgccgggga tcgaagaagg acacagcgca cttggcgtgg tcctttatgc
     9361 ccagggccat tatccgcagg cgattgcgga acttcggacc gcacttaagc ttaagccggg
     9421 cgaccgcaac gcggaagaaa gcctggcgca ggcatacagc cagaccaatg cgatggaatc
     9481 ggcgattgca atatttgaga acatggatcg cgaagcgccg ctgagggccg agttgctcgc
     9541 tatgtgggcg cgggatctgg ctgcacttgc gaacccactc accgccattg atgtgatggc
     9601 ccgggccgtt gcggcagacc cgcagaacgc agaactagtg gatcagttgg gttcgcttga
     9661 tgctcaggcg gggcgttggc agaatgccga gacagcattc cacaaggcgc tcactattga
     9721 ctcaggactg gcctcggcgc acatgcattt ggccgtggta ctgagtcacg agcaacgaac
     9781 gggcgaagcc gagatcgagt tcgctcgcgc gtcgcagatc gatcccggca ccgcgaccgt
     9841 tcaattcgag tggggcaacg cactggtggc agccaatcag gacgatcagg cgattccgca
     9901 ctttcagaag gccatggcgc tggagccgaa ccagatcgac gccgagtatc agctggccct
     9961 ggtgttgcag cgagcgggca aggaagagga tgcggttccg ctccttcaga aagtggtggc
    10021 gtcacgccca cagcaggccg agccgcttac gaatttagga cttgcgttgg tacagacggg
    10081 tcatgcgaag gaagcgattc cgttgtatca gcgggctgcg atcctcagcc cggagaaccc
    10141 gaccgttcat gaggatcttg gcgccgcata tctccaggaa aataacatcg acgatgcgat
    10201 cggagaactc caaacaggcc tgcgcatcgc tccgcaggat cctcatttgc actatgatct
    10261 cgggctggcc tataagctca aagatgattt gccgcacgca attgtcgaac tcgaggcggc
    10321 tctgcggctt gagccggatt cgcccgatgc gccctacaca ctcggcattc tttacatgca
    10381 agacggtcac ttcgatgaag ctgtgcagaa tctccagact gccctgaaag cgcgtccgca
    10441 aaacggcgat ggatggtcca tcctgggcag catctacaga cagcagggca aatatgacga
    10501 ggcagccaat gccctgagaa gagccattga gttagttcct gagcagccgg gtccacatat
    10561 caccctggcc agcgttctgc aggatgaggg taagcaggaa gatgcagcag ccgagcgaaa
    10621 gaaagcagcc gagttgaccc gcgtggcagt aaatcggcag cgcgctatgt ttgcgactaa
    10681 tgccggaaat gcgtttcttg acaaaggcgc cactgccgat gcgatcgttc attatcaaga
    10741 ggctatcagt agcgatccca gctacgcaga agcacatcgg caacttgccg cggcattgct
    10801 gcagcaaggt cggatggctg aggcggcagc ggaacggcag aaggccgaag cgctgactgc
    10861 aaagaatcca taggagtgcg gtggcttcct tctgatgaat cgacgaaaat tcctgacttc
    10921 cctcagtcgc agtgccctgg ttcttccctt tgccgatgtg ttgcagttgg cgaagccttt
    10981 gtggtggaac aaagcgctca tggctgggca gcaagcatcg tcgcccaggc cgggagcgca
    11041 gcaatcgtat gacgcaaagc cgatgccccc accgcccggc ccgacttccc ccgttgcggg
    11101 gactccgttg ggagttcagt tcgtcgacgt cgcaatacag agcggcctcc acgcgaaaac
    11161 gatttttggc ggggaaggga caaacaaata tctgctcgaa acgacgggct gcggcgtcgc
    11221 cttctatgac tatgaccagg atggctggct cgatatcttt ctggtgaacg gttggcggct
    11281 tgagggattc cctaagggga atgagccaac gtgtcgcctc tttaagaata atcgtgacgg
    11341 gacgtttacc gacgttacag cgaaggccgg tgtcggtcac agtggatggg gccaggcttg
    11401 ctgcatcggt gactatgaca atgacggctg ggatgatctg tttattacct actacggaca
    11461 gaatgttttg tttcggaaca acggaaacgg cacgttcacc gatgtaaccg cgaaagctgg
    11521 attgctgcag ccaaaaatgc gctggaattc cggatgcgcc tttcttgact atgatcgcga
    11581 tgggcacctg gatttgtttg taggaaacta tatcgatttc gatctcaaga cagcgcctct
    11641 tccggagtcc ggaccttgtt tatacaaagg catacttgta gcctgcggtc caccggggct
    11701 cagcccggga aagaatattc tttaccacaa caacggcgac ggtaccttcc tggatgtatc
    11761 ggagaaggcg ggaatgggga cgacactggg caattacgcg ctcagcgcag cggtcgccga
    11821 ctttgacaac gatggctggc cggatatcta cgtcgctaac gattccaccg cggcgacgct
    11881 ttatcaaaac caaaaagacg gaacgtttcg tgacgtggcg atcgaagatg gcgtggcttt
    11941 ctcaccagat ggcaggccgc aagcgggtat gggcgtgacc gtgggtgact acaatcgcga
    12001 cgggtggctc gacatcgcca aaacaaattt tgcgggcgat acggattcgc tctacgcggg
    12061 attcggtggc ggcaattttg aagaccgcac gtatcaggcg ggcctgggcg tcaacacccg
    12121 atatcttggc tgggggatag ggttcgtcga tatcgataac gatggatggc ttgacctgtt
    12181 tgttgctaat ggacatgtct atcccgaggt gagcagcgtg aagactgaag cgccatacgc
    12241 tgaacataag tatctctatc gcaatctgcg caatggacgc ttcgaagacg caagtgcact
    12301 gggtggaacg ggcatcaccg ctgccgcatc ggcacgaggg tgcgcctttg gcgactatga
    12361 caacgatggc gacctcgatg tggtggtgaa ttgcgtgaac agcatccctc agctccttcg
    12421 gtgcgattcg ggcggcaagc gtcactgggt aaaggtgaag ctggttggca cgaagtcaaa
    12481 tcgcaccggg atcggcggac gcgtgcgcgt gacggcacat ccccagacgg accagtcgat
    12541 ggtgttcatc gacgaggtgc gaagcggtgg cagctacttt tcccaaagtg acttgcgcct
    12601 gcattttggg ctggatcagg cagagtcgat cgatgtactt gaagttcact ggccctctgg
    12661 tcaggtggat actttcagag gtttgcctgt ggaccggttg tatgtgatcg aagagggtgc
    12721 gaaagagccc aggggatttc tgttgccctc gatgaggaag gtgtgaagtg acgctaagat
    12781 ctcgaaccgc gaatcttctc gcggcatttc tctgcgctgg attcctgtcg gggacggctt
    12841 cgtctggcga tacggaatcc tggcagattg gtccgttcac tcgcccaatt gtcggcaatc
    12901 cggtaatcaa gcccaatccg gccgcgctct ttaccgatcc aatccgcaag actgcggtgc
    12961 gctgggagtc gttgcatacc ttcaatcctg ccgcggtagt tcgcgacggc atgattaacg
    13021 tgctctatcg cgctgaagac gatacgggtg cgatgcaaat tggtatgcac acgtcgcgcc
    13081 tgggacgggc gcagagtgtg gacgggactc acttcacccg cagcgaaacg cccgtattct
    13141 acccggaagc cgatagtcag caagcccggg agtggcccgg aggtgtcgaa gatccccgca
    13201 tcgtcgaagc cgaggatggc acttatgttc tgacttatac ccagtggaac cgcatcacct
    13261 actctgtcgg aatcgctacg tcaccggacc tggagcactg gatcaaacac gggccggcct
    13321 ttttcaacgc cgcaggagga aagtacatca accttaagta caagtcggca ggtattgtga
    13381 ctgctctgcg tggtggaagg ttgattgctg tgcgcattga cggaaagtac tggatgtact
    13441 ggggggaggg tgctatccac ctggccacgt cgtcggatct gattcactgg tcgcctgtag
    13501 aaggtgcgga tagcgaaccg cttgaggttc tcgaaccgcg gcgaggacgg tttgacagct
    13561 cattcccgga gaccggcccc ccgccaattc ttacatcgcg gggcattgta atgatctata
    13621 acggcaagaa cgctgccaca ggaggcgacc cacaactggg tgccaacact tatgcggtgg
    13681 gcgaggcgct attcgacgcg cgcgatcctg ccaggttact cgcccgaacc gatagcccgg
    13741 tcctagagcc cgttttgcct tatgagcgaa cggggcaata ttccgcgggc acaacgttcg
    13801 ccgaggggct ggtgtacttc cacaaccagt ggtttctcta ctacggttgc gccgattccc
    13861 tggttgccgt tgcgacagcc ccggcaaacg ttacaaaatg atcgggacac aactcatccg
    13921 agttgtggcc tgagagcaaa gtttagagca ggacggctaa aaggtgatcc gtcccgagag
    13981 ttggaaatc
//
LOCUS       DASZYN010000030         1680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_194460, whole genome shotgun sequence.
ACCESSION   DASZYN010000030 DASZYN010000000
VERSION     DASZYN010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194460"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VHE33_00870"
     CDS             <1..775
                     /locus_tag="VHE33_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HVT96026.1"
                     /translation="PASVERSRPVPQVALFAGCIMSTAFAETGRATLRVLQAHGCAVE
                     LPAGQGCCGALTVHAGDLEGARRMARRNIEAFERSGLAFIIVNAAGCGAALKEYAHLL
                     RDDPVYAERAAQFSARVRDVSEFLSEQPAPRQVRALPLRVTYQEPCHLAHAQRISKAP
                     RQLLGRIPDLELREMNESSLCCGSAGIYNLTQPAMSRRLRARKIQHTSATDAEIVITA
                     NPGCQLQLQSGLRQARSPMRVLHLVDLLDMAYGPDETAP"
     gene            1030..>1680
                     /locus_tag="VHE33_00875"
     CDS             1030..>1680
                     /locus_tag="VHE33_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HVT96027.1"
                     /translation="MTFINLGEWLARRTLLTPEKIGLIDSASGQRLSYRALDVRARAL
                     AAYLERQYHVTQGERVAVLALNAPEYLDAFFACAYLGAILVPLNWRLTAHELGVILAD
                     CQPALLLHDQEYSERAREVARNARPDLDLPLCAFSAFPGERIELANQARPFVSADGEE
                     PVLILYTSGTTGVPKGVILSHRMITWNAINTQISWGLRESDITPTFAPFFHAGGLNV"
BASE COUNT          297 a          546 c          504 g          333 t
ORIGIN      
        1 ccctgccagc gtggagcgca gccggccagt cccgcaagtc gcgctctttg ccggctgcat
       61 catgagcacc gcctttgctg aaaccgggcg ggctactctg cgcgtcttgc aggcccacgg
      121 ctgcgcggtt gaactgccgg ctggccaggg ctgctgcggc gccctgaccg ttcatgccgg
      181 cgacctggag ggtgcgcggc gtatggcccg gcgtaacatc gaggcctttg aacgctccgg
      241 cctggctttc atcatcgtca atgccgccgg ctgcggggcc gcgctcaaag agtacgctca
      301 tctgctgcgc gacgatccag tatacgccga acgggccgcg cagttcagcg cccgcgtgcg
      361 cgatgtcagc gaatttttga gcgagcagcc cgctccccgc caggttcgcg ccttacccct
      421 gcgcgtcact taccaggagc catgtcacct ggctcatgcc caacgtatca gcaaagcgcc
      481 ccggcaactg ctcggacgca taccggacct cgaactacgg gaaatgaacg aatcttccct
      541 ctgctgcggc agcgcaggca tttacaatct cacccagccc gccatgtcac ggcgactgcg
      601 tgctcgcaaa atacagcata ccagcgccac cgatgccgag atcgtcatca ccgccaatcc
      661 aggctgtcaa ctgcaactgc aaagcggtct gcgccaggcc cgttccccca tgcgcgtgct
      721 gcacctcgtc gatctcctgg acatggccta tggcccggac gaaaccgcgc cctgagctgg
      781 agatcatgcg tcccgggtga actgcctgcc ctcttctcaa accggctctg catgctcgtc
      841 gttatcggcc aatccttgtt cttccggctt ttgaggcgtt ccgggcaggc tctcagagga
      901 cggacggagc gcgtcatctc tatctccccg cttatggtat gatatatgaa ccatatgtca
      961 tattaccagg gagaagccgg agaagtccgc ctggtaaaaa gaacgctgta gttctcacgg
     1021 aggtgcttta tgacgttcat caaccttggc gaatggctgg cgcggcgcac gttgttgacc
     1081 ccggagaaga tcgggctgat tgacagcgca agcggacagc gtttgagcta tcgcgcgctg
     1141 gatgtgcgag cgcgggcgct ggcggcctac ctggagcggc agtatcacgt cacgcagggg
     1201 gagcgggttg cggtgctggc tttgaatgcg ccagagtacc tggacgcctt tttcgcctgt
     1261 gcctacctgg gggccatcct tgtcccgctc aactggcgac tgaccgcgca tgagctgggc
     1321 gtgatcctgg ccgattgcca gccggcgctg ttgttgcacg atcaggagta tagcgaacgc
     1381 gcgcgtgaag tggcgcggaa cgcgcggcct gatctcgatc tgccgctgtg tgctttcagc
     1441 gccttccccg gcgagcggat cgaactggcg aaccaggccc ggccttttgt cagcgctgat
     1501 ggcgaggagc cggtgctcat tctctacacc tcgggcacga ccggcgtacc caagggcgtc
     1561 atactttccc atcgcatgat tacctggaac gccatcaaca cccagattag ctggggactg
     1621 cgggaaagcg acatcacgcc caccttcgcg cccttttttc atgccggagg cttaaatgtg
//
LOCUS       DASZYN010000031         8787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_836902, whole genome shotgun sequence.
ACCESSION   DASZYN010000031 DASZYN010000000
VERSION     DASZYN010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8787
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836902"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VHE33_00880"
     CDS             <1..358
                     /locus_tag="VHE33_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HVT96028.1"
                     /translation="LRTDVNLAWPTAEIAVMGPEGAVNIVYGRELAHTVKLAEKSEGP
                     LSEERRAEVLSAARTTRVHQFREQFANPYVAAERGYVDAVILPHETRARLCSALDMLE
                     GKREKMPPKKHGNIPL"
     gene            716..2263
                     /locus_tag="VHE33_00885"
     CDS             716..2263
                     /locus_tag="VHE33_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family serine peptidase"
                     /protein_id="HVT96029.1"
                     /translation="MSSPAQKRVVLSRSERNVPANASLVGRTDPKQALSVSVIVKRKN
                     SLDLHALAGQRVSREDFDAQFAADPADFEALRVFAHDHGLTVDEAASSLARRTLVLRG
                     PVEAMEKAFGVELHDYKDGRTKKRYHCFTGHVSLPEAHAPVVDAVLGLDARPLAKPHF
                     RYLKNLKKKKPKAAPAPVPFNPPQVAALYKFPTGVNGAGQTIGIIELGGGYTTSDITQ
                     YFGGLGIKPPTVVAVSVDGGTNAPGDPNGADGEVALDIQIAGAIAPGAKIAVYFAPNT
                     DQGFIDAITTAVHDTANKPSVISISWGAPETSWAQSSVTALDDACQSAAALGVTITVA
                     SGDNGSSDGVSDGQNHVDFPASSPHVLACGGTELIGSGSTIGSETVWNDGAQGGATGG
                     GFSTLFPLPAWQTGAGISGSGRGVPDVAGDAAPESGYNILVDGQQEVVGGTSAVAPLW
                     AALVALLNQQKGAPVGLMNSTLYGEAADFRDITQGNNGTYKAAKGWDACTGLGSPNGQ
                     AIAKALE"
     gene            2344..3486
                     /locus_tag="VHE33_00890"
     CDS             2344..3486
                     /locus_tag="VHE33_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96030.1"
                     /translation="MADPENPFIFGEIIEEPNFVDRTGELHQLVRDLADGQKVFLLSP
                     RRFGKSSLVALAMLKLRKRHIRTVSLTVSSYASYAQFLEKFAEKVLRAAGPWERVKDW
                     VTRFGRNVKPDISYNMSSGEVSVSLGRGPRFDPTPIAPDVFALPGELARNAGFRMAIC
                     LDEFQQVAEFDSGTVENVIRNQAQKQREVAYVFAGSQPSLMKEMLSARRPFHKAGPVI
                     YLDKIPADEWKTFIRRQFARRGRKVEDDAVEELLATADLIPYDVQRLAHELWDYAELS
                     DRSSLSLRDVRMVTDRLVASQSTYYELLWQQLAVGQRAVLQAMAQRGPVDIYGEAVRA
                     EFRLGAASSVQRALQALDMKDVIDRYQDRYFFLDPLFACWIRKRAL"
     gene            complement(3573..3728)
                     /locus_tag="VHE33_00895"
     CDS             complement(3573..3728)
                     /locus_tag="VHE33_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96031.1"
                     /translation="MHELIAHLSELSSYLPAILITLGVAALVACAVGCHASPCEEKDD
                     LFHREVY"
     gene            complement(4131..5126)
                     /locus_tag="VHE33_00900"
     CDS             complement(4131..5126)
                     /locus_tag="VHE33_00900"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HVT96032.1"
                     /translation="MTPGRRLTDLKLDAAYQKCTEIARRQAKNFYYAFLALPKPKRDA
                     ICAVYAFMRHADDLSDDESRSREERRADLAAWLAEWHSAASGEPTSDPVFMALADTQA
                     NFQIPLELLDQLVQGTAMDLVPRAPQIADANSAAEPVPYNNYTTFATFEDLRRYCYYV
                     ASVVGLVCIRIFGYSDPRAESLAEDTGVAFQITNILRDVREDAERGRIYLPLDALDRF
                     GVRIENLAALRDGAHMTLNQRELLESLAKRAEDYYKSGFALLPLIDADSRPALRVLVT
                     IYHSLLRRIQRRGYDVFSERVRVPLSTRLAILFRGLLQVLRYRLFGPSQSGRDQA"
     gene            complement(5164..6207)
                     /locus_tag="VHE33_00905"
     CDS             complement(5164..6207)
                     /locus_tag="VHE33_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HVT96033.1"
                     /translation="MAAQMTAAVLHGREDLRIEQVPMPKAGVGEIVVRVHAALTCGTD
                     LKVYRRGYHARMLKPPIPFGHELAGTVHQAGRGVAAFRAGDRVVALNSAPCGDCYYCR
                     HQQENLCDRLIFNNGAYAEYLLIPSRIVAKNTLRIPTGISFEHAALTEALACVVHGLD
                     ECQPHSGDTVAIIGAGPIGLMFMHLAELSGLNVIAVVKRDEQVAAAKRFGASEVIQIS
                     PTIDPVAAVRALTPQRRGVDIAIEAVAKPIAWQWAVDMVRKGGTVNFFGGPAAGTKVE
                     LDTNRLHYNNITLRSSFHHTPAAARKAFDLIVSGQFRSSDTITSRAPLSELNRVFRRL
                     MNRAGDIKTAIIP"
     gene            complement(6220..7344)
                     /locus_tag="VHE33_00910"
     CDS             complement(6220..7344)
                     /locus_tag="VHE33_00910"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent dehydrogenase"
                     /protein_id="HVT96034.1"
                     /translation="MATATTNDLRKGTSAPIPATMRAAVYRGINDVRVETVPVPEIGP
                     GEVLIRVHSCGVCGTDLKKIHTGSHSAPRIFGHEISGVIAATGEGVRGFEAGDRVMVF
                     HHTPCGKCYYCRHKTPSQCNDYKRVGVTAGFEPSGGGFAEYVRVMPWIVGRGLIKLPD
                     HVSFEQAAFVEPVNTVYKAIRNLALEPDETVLVIGQGPIGIMHAALAARTGAKVLTSD
                     LYPERHRIAAQYGLDHPIDASKEDVVAVAHRESEGRGADAVIVAVGGNSLIKTAMDAA
                     RPGGRVLLFAATQHCEATFDPAVVCMDEKTLMGSYSSSFDLQPEVEEIVFGGYRTDFD
                     LTRLISHRFALEDAVEAIRVASNPSAASMKVFVQPEAAQV"
     gene            complement(7360..8070)
                     /locus_tag="VHE33_00915"
     CDS             complement(7360..8070)
                     /locus_tag="VHE33_00915"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96035.1"
                     /translation="MNRIALIAALPSELRPLVHHWPRHGKISTGRIGNVDAVALASGM
                     GESAVTRACEAVLADGTCDTLVSIGYAGSLSCGLRAPDAVAVSEIVDARTGETFTATH
                     PAAQPAGGLKPQRLVTLDHVANPEEKRRLAETHQATLVDMEAATVARFAQTHNLGFLC
                     FKAITDGPNDELPDFNRFTTSDGRLRTRAFAAWALVHPRYWRILSELEKNSHAAAREL
                     AKFITANFASPSPLASVS"
     gene            complement(8111..8195)
                     /locus_tag="VHE33_00920"
     tRNA            complement(8111..8195)
                     /locus_tag="VHE33_00920"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8159..8161),aa:Leu,seq:tag)
     gene            8255..8545
                     /locus_tag="VHE33_00925"
     CDS             8255..8545
                     /locus_tag="VHE33_00925"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HVT96036.1"
                     /translation="MNPPQPKIQLTQNEEYREIYSNSVQVRVSVWDFLLVFGLVHQET
                     PEQVNVNNALGVYLSPQQAKALWNILGQNIAQYEQTFGALALEPQPPKGPVH"
     gene            8557..>8787
                     /locus_tag="VHE33_00930"
     CDS             8557..>8787
                     /locus_tag="VHE33_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96037.1"
                     /translation="MRGTSRAVPWWIVLPGAFAAVLAAHAPLLHLPYYWDEAGYYIPA
                     AWDFFRTGSLIPFSTLSNAHPPLPSLILAGWWK"
BASE COUNT         1686 a         2280 c         3249 g         1572 t
ORIGIN      
        1 tctgcggacg gacgtgaacc tggcatggcc gacggccgaa atcgcggtga tgggaccgga
       61 aggcgcggtg aacatcgtgt acgggcgtga gctggcccac accgtaaagc tggcggagaa
      121 gagcgaggga ccgctgagcg aggagcggcg tgcagaggtg ctgtctgcgg cgcgcacgac
      181 gcgcgtgcac cagttccggg agcagtttgc caacccgtac gtagcggcgg agcggggata
      241 tgtggacgcg gtgatcctgc cgcacgagac gcgggcacgg ctgtgctccg cactggacat
      301 gctggaaggg aaacgggaga agatgccgcc gaagaagcat gggaacattc cgctgtaaga
      361 gcagctgaag tccggtcggc taagaggcgg tcagctaaaa agcggttagc taaggtccgg
      421 tcagctgaag tccggtcagc taagaagcgg tcagctaagg tccggtcagc tgaaaagcgg
      481 ttagctgaaa gccggttggc ggagaagagg cgcgcctgcg gcgcgtgagg ccgacatctc
      541 caacggcgga gatgcggggc acccggcatg aggcccagcc tggcgctgtg ggcgggcaca
      601 ctgtgggcgg gcacaaccta attgccgcgt cccgatgtct aagttctggt cttatccata
      661 gatttggcgc agacgtgttt gcgctacgat ggctgcacat cgtggaggtt aggccatgag
      721 ttcccccgct cagaagcgtg tagtcctgtc ccgttccgaa cgcaatgttc ccgccaatgc
      781 aagcctggtc ggacgcaccg atccgaagca ggcgctcagc gtttcagtca ttgtcaaaag
      841 gaagaactcg ctcgatctgc acgcgctggc ggggcagagg gtgtcgcgcg aggatttcga
      901 cgcccagttt gctgcggatc ccgccgattt tgaggcgttg cgcgtgtttg cgcacgatca
      961 cggtttgacg gtcgatgagg cagcgtcgag cctggcacgc cggaccctgg tgctgcgagg
     1021 gccggtcgag gcgatggaga aggcgtttgg cgttgagctg cacgattaca aggacgggag
     1081 gacgaagaag cgctaccact gtttcaccgg acatgtgagc ttgccggagg cgcatgcgcc
     1141 ggttgtggac gcggtgctgg ggctcgatgc gcgaccgctt gcaaaacccc actttcgcta
     1201 cctgaagaac ctgaagaaga aaaagccgaa ggccgcgccg gccccggtgc ccttcaatcc
     1261 gcctcaggtc gcggcgctgt acaagtttcc caccggcgtc aatggcgcgg gtcagacgat
     1321 cgggatcatt gaactgggcg gagggtacac gacgagcgac atcacgcagt actttggcgg
     1381 gctggggatc aagccgccga cggtggtggc ggtgtcggtg gacggaggga cgaatgcacc
     1441 gggcgatccg aacggcgcgg atggagaggt ggcgctggat attcagatcg cgggggcgat
     1501 tgcgccggga gcgaagattg ccgtgtactt cgcgccgaat acggatcagg ggttcatcga
     1561 tgcgatcacg acggcggtgc atgacacggc caacaagccg tcggtgatct cgatcagctg
     1621 gggggcgccg gagacgagct gggcgcaatc gtcggtcacg gctctggacg atgcgtgcca
     1681 gagcgcggca gcgctggggg tgacgatcac ggtcgcgtcc ggggacaacg ggtcgtcgga
     1741 cggagtgagc gacggacaga accacgtgga ttttccggca tcgagcccgc acgtgctggc
     1801 gtgcgggggg acggaactga tcggatcggg atcgacgatt ggatcggaga cggtgtggaa
     1861 cgacggcgct cagggtgggg cgacgggcgg cggtttcagc acgctgtttc cgctgccggc
     1921 atggcagaca ggcgcgggca tctcgggaag cgggcggggc gttccggatg tggccggcga
     1981 tgcggcaccg gagagcggat acaacatcct ggtggacggg cagcaggagg tggttggggg
     2041 aacgagcgcg gtggccccgc tgtgggcggc actggtggcg ctgctgaatc agcagaaggg
     2101 agcgccagtg ggcctgatga attcgacgct gtacggagag gcggcggatt ttcgcgacat
     2161 cacccaggga aacaacggga cgtacaaggc ggcgaagggc tgggatgcct gcacagggct
     2221 gggctcgccg aacgggcagg cgatcgcgaa ggcgctggaa taaggttacg caaaagagcg
     2281 ttacgcagaa atgcgtaatt atgtggacga attacgcaaa agagcgttac gctgaatagc
     2341 gtaatggctg atccggaaaa cccattcatt ttcggcgaaa ttattgagga gccgaatttc
     2401 gtcgaccgca ccggcgagct gcaccagctg gtgcgcgatc tggcagacgg ccagaaggtg
     2461 tttctgctct ctccgcgccg tttcggcaag agctcgctgg tagcgctggc catgctcaag
     2521 ctgagaaagc gccacattcg cacggtcagc ctgacggtga gcagctacgc gagctatgcg
     2581 cagttcctgg agaagttcgc ggagaaggtg ctgcgggcgg cgggtccgtg ggagcgggtc
     2641 aaggactggg tgacgcgctt cggccggaat gtgaagccgg atatcagcta caacatgagc
     2701 agcggagagg tcagcgtgtc gctgggccgg ggcccgaggt tcgatcccac tccgatcgcg
     2761 cccgacgtat tcgcccttcc tggagaactg gcccggaacg ccggctttcg gatggccatc
     2821 tgcctggacg agtttcagca ggtggctgaa ttcgacagcg gaacggtgga gaacgtcatc
     2881 cgcaatcagg cgcagaagca gcgagaggtg gcgtatgtat tcgccggttc tcagccttcg
     2941 ctgatgaagg agatgttgtc ggcgcgccgg ccctttcaca aggccgggcc ggtgatctat
     3001 ctggacaaaa ttcctgcgga tgaatggaag acttttatcc gtcgccaatt tgcacggagg
     3061 ggaagaaagg tggaagacga tgctgtggaa gagcttctgg cgactgccga cctgattccc
     3121 tacgatgtgc agcggctggc gcatgaactg tgggattacg cagagctgag cgacagaagc
     3181 agcctgagcc tgcgcgatgt acggatggtg accgacaggc tggtggcgag ccagtccacg
     3241 tactacgagc ttctatggca acagctggcc gtggggcaga gggcggtact gcaggcgatg
     3301 gcgcagcgcg ggccggtcga catctacggg gaagcggtgc gggcagagtt ccggctggga
     3361 gcggcgtcca gcgtgcagcg ggcgctgcag gctctggata tgaaggatgt gatcgatcgc
     3421 tatcaggatc gatatttctt cctggatccg ctgtttgcct gctggattcg gaagcgggct
     3481 ctctaagcaa cgcctttcag cggtgcgcaa aaaaaagcgt aaagatggtc aggcattcct
     3541 gggagaggtc cgccaccgag gcggttgccg gctcaataca cttcgcgatg gaagaggtcg
     3601 tccttctcct cgcagggtga agcgtggcag cccacggcac aggcaacgag ggcggcgacg
     3661 ccgagagtga tgagaatggc cggcagataa ctgctcagtt ccgacagatg ggcgatcagt
     3721 tcatgcatac agaggaagcc ccatttcgct cccacgcatt gggacacctg aattcgtgtt
     3781 caggttttct tcaatcaacg ggagtggcag ccatgttggc agaatttccg ggggatggaa
     3841 ataacattct aggcatgtaa ctacaggatt ggcggcgcat tttccacatt tgccaagagt
     3901 tggggcggaa tcgcactgcg gatgtgagtc aggcaggctt cttgtcgcga ttgatcggat
     3961 tgaaagtaga agattctctg aggcttgcgg gcaaagctgt caaaagacga caggcgctgg
     4021 gcagcagggc aacggtttcc cgacttggga gaggtacgtt accagtgggg gtgccttgcg
     4081 gaccgaacga gcgagccaag gcccgtctga agggtttccg acattgtcct tcaggcctga
     4141 tctcgtccgg attgggaggg gccgaagagg cggtagcgca gaacctgcag aaggccacga
     4201 aagagaattg cgaggcgggt agacagtggg acccttaccc gttcggagaa gacatcgtag
     4261 ccgcggcgct gaatgcggcg gaggagggag tgatagatgg tgacgaggac ccggagagca
     4321 ggccgggagt cggcgtcgat cagaggcagg agcgcgaaac cggacttgta gtagtcctcg
     4381 gcccgtttgg cgagggactc gagaagttcg cgctggttga gggtcatgtg ggcgccgtca
     4441 cggagggcgg cgagattctc aatgcggaca ccgaagcggt cgagggcatc gagcgggaga
     4501 tagatgcggc cgcgttcagc gtcttcacga acatcgcgga ggatattggt gatctggaag
     4561 gcgacgccgg tgtcttcggc gagggattcg gcgcgtggat cggagtagcc gaagatgcgg
     4621 atacagacga ggccgacgac ggaggcaacg tagtagcagt aacggcggag gtcttcgaag
     4681 gtggcgaagg tcgtgtagtt gttgtaggga accggctccg cggcgctatt ggcgtcggcg
     4741 atttgggggg cacggggcac cagatccatg gctgtaccct ggacgagctg gtcgagcagc
     4801 tcgaggggga tctgaaagtt ggcctgcgtg tcggcgaggg ccataaagac ggggtcgctg
     4861 gtgggctcgc cggatgccgc actgtgccat tcagccagcc aggcggccag atcggcacgg
     4921 cgctcttcgc gggagcgcga ctcgtcgtcg gagaggtcgt cggcgtgacg catgaaggcg
     4981 tagacggcgc agatggcatc gcgtttgggt ttgggaagag cgaggaaagc gtagtaaaag
     5041 ttcttcgcct ggcggcgtgc gatctcggtg cacttttggt aggcagcgtc gagtttgaga
     5101 tcggtgaggc gacggccggg cgtcacgcct ggctccggga aatgccggtc aacgttccgc
     5161 tccttaggga atgatggcgg ttttgatgtc gccggcgcgg ttcatcaggc ggcggaagac
     5221 gcggttcagt tcggaaagcg gagcgcggct ggtgatggtg tcgctggagc ggaattgtcc
     5281 gctgacgatc aggtcaaatg ccttgcgggc ggcggcgggt gtgtggtgga agctggagcg
     5341 gagggtgatg ttgttgtagt gaagccggtt ggtatcgagc tcgaccttgg tgccggcggc
     5401 ggggccgccg aagaaattga ccgtgccgcc tttgcggacc atatcgacag cccactgcca
     5461 ggcgatgggc ttggcgacgg cttcaatggc gatgtcgacg ccgcgtcgct gcggggtgag
     5521 ggcacggacg gcagcgaccg gatcgatggt ggggctgatc tgaatgactt cggaggctcc
     5581 gaagcgtttg gcggcggcca cctgctcgtc gcgtttgacg acggcgatga cattgaggcc
     5641 actcagttcg gccagatgca tgaacatgag gccgatgggt ccggctccga tgatggccac
     5701 ggtgtccccg gagtgcggct gacattcgtc cagtccatgg acgacgcagg cgagggcttc
     5761 ggtgagggcg gcgtgctcga aggagattcc ggtggggatg cggagcgtgt tcttcgcaac
     5821 gatgcgagag gggatgagca ggtattccgc ataggcgccg ttgttgaaga tgaggcggtc
     5881 gcagagattc tcctgctggt ggcggcagta atagcaatcg ccgcaggggg cggaattgag
     5941 ggcaacgacg cgatcgccgg cgcgaaaggc agcgacgccg cgaccggcct gatggacggt
     6001 gccggccaac tcgtgtccaa agggaatggg aggcttgagc atgcgcgcgt gataaccgcg
     6061 gcgatagacc ttgaggtcag tgccgcaggt gagggctgca tggacacgga ccacaatttc
     6121 gccgactccc gccttcggca tgggaacctg ctcgatgcgc aggtcctccc ggccgtggag
     6181 gacggcggct gtcatttgcg ctgccatctt tctattctct caaacctggg ctgcttcggg
     6241 ttgaacgaac actttcatgg aggcggcgga gggattcgaa gcgacacgga tggcttccac
     6301 ggcgtcctcc agcgcaaagc ggtgggaaat gaggcgggta aggtcaaaat cggtgcggta
     6361 gccgccaaaa acgatctctt cgacctccgg ctggaggtca aaggaggagc tgtaggagcc
     6421 catgagggtc ttttcgtcca tgcagacgac ggcagggtcg aaggtggcct cgcagtgctg
     6481 ggtagcggcg aagagcagga cgcggccgcc ggggcgggcg gcatccatgg ccgttttgat
     6541 gagggaatta ccgccgacag ccacgatgac ggcatcggca ccacggcctt cgctctcgcg
     6601 atgggcgacg gcaacgacat cttctttgct ggcgtcgatg gggtggtcga ggccgtactg
     6661 ggcggcgatg cggtggcgct ccggatagag gtcggaggta aggactttgg cgccggtgcg
     6721 ggcagcgagg gcggcgtgca tgatgccgat ggggccctgg ccaatcacga ggacggtttc
     6781 gtcgggttcc agagccaggt tgcgaatggc tttgtagacg gtgttcaccg gctcgacgaa
     6841 ggccgcctgt tcgaaggaga cgtggtcggg cagtttgatg aggccgcggc cgacgatcca
     6901 gggcatgaca cggacgtatt cggcgaagcc gccgccggag ggctcaaaac cggcggtgac
     6961 accgacacgc ttgtagtcgt tgcactggga gggggttttg tggcgacagt aataacattt
     7021 gccgcacggc gtgtggtgga agaccatgac gcggtcgccg gcttcgaagc cgcggacacc
     7081 ctcgccggtg gcggcgatga cgcccgagat ttcatgcccg aagatgcggg gagcggagtg
     7141 ggagccggtg tggatcttct tgagatcggt gccacagaca ccgcaggagt gaacacgaat
     7201 gaggacctcg ccgggaccga tttccggtac cggtacggtc tcgacgcgca catcgttgat
     7261 gccgcggtag acggcagccc gcatggtggc ggggatgggc gcagaagtac ctttacgcag
     7321 atcgtttgtt gtcgctgtag ccatggggac tctaaagaat cagcttaccg aagccagcgg
     7381 ggagggggaa gcaaaattcg cggtgatgaa ttttgccagc tcgcgggcgg cggcgtggct
     7441 gttcttctcc agctcgctca ggatgcgcca gtagcgggga tggacgaggg cccaggcggc
     7501 gaaggcgcgg gtgcggaggc ggccgtcgga ggtggtgaag cggttgaagt cgggcagctc
     7561 gtcgttgggg ccatcggtga tggctttgaa gcagaggaag ccgaggttgt gggtctgagc
     7621 aaagcgagcg acggtggccg cttccatgtc gacgagggtg gcctggtggg tttcggcgag
     7681 gcggcgcttt tcttccgggt tggcgacgtg gtcgagggtg acgaggcgct ggggcttgag
     7741 tccgccggcc ggctgagcgg ctgggtgggt ggcggtgaag gtttcgccgg tgcgcgcgtc
     7801 gacgatctcg ctgacggcga cggcgtccgg tgcgcgcagt ccgcaggaga gcgagccggc
     7861 gtaaccgatg gagaccaggg tgtcgcaggt gccgtccgcg aggaccgctt cacaggcgcg
     7921 agtcacggcg gactcgccca tgccggaggc gagggcgacg gcgtcaacat taccgatgcg
     7981 cccggtggag atcttgccgt ggcggggcca atggtgtacg agggggcgca gctccgaagg
     8041 aagggcggcg atgagggcga tgcggttcat agctggaggt cagaaaccat ggaagcggaa
     8101 cgcaagcgtg tggtgcgaaa ggggggactc gaacccccat gggttgcccc gccagatcct
     8161 aagtctggtg cgtctgccag ttccgccact ttcgcacgcg ggtagcagct tcattgtagc
     8221 ccgcagtaaa cggtgcggta gtctggtggg gtctatgaat ccgccgcaac ccaaaatcca
     8281 gctcacgcag aacgaggaat accgcgaaat ctactccaac agcgtccagg tgcgcgtcag
     8341 cgtttgggat tttctcctcg tcttcggtct tgtgcatcag gaaacgccgg agcaggtgaa
     8401 cgtcaacaac gctctcgggg tctatctgag cccgcaacaa gccaaggcgc tgtggaatat
     8461 cctcggacag aacatcgcgc aatatgagca gacctttggc gctctggcgc tcgagccgca
     8521 gccgccgaag ggaccggttc actgaggccc atcgcggtga gggggaccag ccgtgctgtt
     8581 ccctggtgga tcgttctgcc gggcgcattc gccgcagtgc tggccgctca cgcaccgctg
     8641 ttgcatctgc cttactactg ggacgaggcc ggctactata tccccgccgc gtgggatttc
     8701 ttccgtacgg gctcgctgat cccgttctcc actttgtcca atgcgcatcc acccctgcca
     8761 tcgctgatcc tggcagggtg gtggaag
//
LOCUS       DASZYN010000032         9144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_322063, whole genome shotgun sequence.
ACCESSION   DASZYN010000032 DASZYN010000000
VERSION     DASZYN010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 9144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9144
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322063"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="VHE33_00935"
     CDS             <1..941
                     /locus_tag="VHE33_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96038.1"
                     /translation="AAPEVAPVAPLRGIPPVPAIAPLPPNPADIEVDDSPDTMVSVDD
                     QDTRGYTYGFSDNGESYAIVEGSGNDVTFSGSWDNHRRAEIDAARKGAKGPFLWFTHE
                     GKSYIVTDPAIIAKIRAMYQPMEELGRKQEALGKQQEALGKQMEELAREQEAAGQVKM
                     PDLSREMAQIDAALAKFKENEHQRNAPALAEAEAKVKAAQDKMLSPERMAELQSDLAA
                     AQAQWNSESMSSLQERLGELQARLGELQGEAGARQGDYGAKMGALGAQQGKLGGEQGR
                     LGAEQGRIARQADRQVRGIIEDCLRNGKASRLPQVK"
     gene            1073..1732
                     /gene="plsY"
                     /locus_tag="VHE33_00940"
     CDS             1073..1732
                     /gene="plsY"
                     /locus_tag="VHE33_00940"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HVT96039.1"
                     /translation="MPVIFYLVAAVVAYLLGSIPFGYLLVRLFRKEDIRQKGSGNIGA
                     TNVMRSGSKGLGALTFLLDVLKGFCAVWLCGAYALHAGLSWNARVNAVAIAALCVVLG
                     HIFTMWLGFRGGKGVATAFGVFIALAPWAALASVGVFAVVFGASRYVSLGSIVSALAF
                     PIIAVLLPHAPRTPLENAVLVVIPLIVILKHHQNIGRLLSGTEYRFGKKQTTEPGTTA
                     V"
     gene            1729..2736
                     /locus_tag="VHE33_00945"
     CDS             1729..2736
                     /locus_tag="VHE33_00945"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HVT96040.1"
                     /translation="MSRIAIMGSGAWGTALAISLARRGGHQIALWSYAKDVAASMRDR
                     RENPQFLPGFRIPESVAVFDNSAEALEGAEIIVSVTPSQFARASYEGFAPYLRPGQLL
                     VSATKGIEDETYLRMTQVIGEVLASRGLELPCGVLSGPSFAQEVAAGAPTAITIASAD
                     ANLASKVQREFSSSTLRLYTNDDVVGVELGGSLKNVIAIAAGIVAGLELGHNSNAALI
                     TRGIAEITRLSVACGGQRETLAGLSGVGDLVLTCTGSLSRNRTVGFGLGQGRKIDEIL
                     AGLAGRVAEGVKTTSAALGLARRHAVEMPIAEQMEAILLHGKHPQRAIEELMLRPGRD
                     E"
     gene            2888..3904
                     /locus_tag="VHE33_00950"
     CDS             2888..3904
                     /locus_tag="VHE33_00950"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HVT96041.1"
                     /translation="MQKVPARAARLSSPGMRIRWRVFARFLALFAGATSVTVLAMMLA
                     QRTLRAVVAGWAVVLIEFAGSAAAALLLTVWMEKRIQRLVDTLASNDVAAGTSDELFA
                     EIIPAWQQNVKALADKANEKTDEARSHYEALKDFMRMIAKAVDERTAYLRGHSERVAA
                     YAAAIAWKLGLDERQVERVRLAALLHDIGCLGVEDYLVMKTTPLTPEEFEIVKAHTVK
                     GAAILRPITMLQDLVPGVELHHESLDGMGYPYGLTGEQIPLIARIVSVADSFDAMTTP
                     RPYQTAMNADYVLDILTRLSGTRYDPVVVIALAELVRTGALEVKNVRTPVSFRMRKPV
                     PELV"
     gene            3979..4944
                     /gene="ftsY"
                     /locus_tag="VHE33_00955"
     CDS             3979..4944
                     /gene="ftsY"
                     /locus_tag="VHE33_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HVT96042.1"
                     /translation="MIQTLFGSLKEEQDPEKQSLLERMRSAVTRTRESLGERIDGVLA
                     LTRTVDEAALEELEMSLIASDIGVNTAAEITNHLRERAKRQQIQDGAELKGLLREELQ
                     AILDENAPPAKSVPRAPEVIMMVGVNGTGKTTTSGKLAAFYRSQGKTALLCAADTFRA
                     AAIEQLEVWGARSGVEMIKTKQGGDPSAVLFDALQAAQTRQTDVVIVDTAGRLHTKTG
                     LMAELDKMRRTAQRIISDAPHEVLLVLDATTGQNGLQQARLFTESAGVTGIVLTKLDG
                     TAKGGIAVAIAREMKLPVRFVGIGEALEDLLPFDSGAFVNSLLEN"
     gene            5021..6166
                     /gene="ribD"
                     /locus_tag="VHE33_00960"
     CDS             5021..6166
                     /gene="ribD"
                     /locus_tag="VHE33_00960"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HVT96043.1"
                     /translation="MRRALELAQHSIGLASPNPIVGCVLVKDDVIVGVGRHEYDKKDH
                     AEIVALKNAGGSARGATAYVTLEPCCHTGRTGPCTKALIEAGVARVVAATADPNPAVS
                     GKGLEQLQAAGIEVAVGVLQCGARALNDGFAKHIRTGLPFVTLKAGLSLDGRIAPAPG
                     SAPHGAPVFLTGDKAREQVQEMRHAADAVITGINTILQDDPQLTDRSGMPRRRPLLRV
                     VLDSGLRIPLDSKLVRTAQDDLLIFCGVATTQRQQTLEALGVRVERIPTAEAVSDQFA
                     TGMPTHGRTARRTGVSLKTVMHRLGEMGILSALLEAGGQLNASALNGGYVDKLTLFYA
                     PVFLGSDGVPLLQEGMLKPLLPTPPLIERVGNDVRIDAWLRNPWGEA"
     gene            6170..6826
                     /locus_tag="VHE33_00965"
     CDS             6170..6826
                     /locus_tag="VHE33_00965"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HVT96044.1"
                     /translation="MFTGIIEATGEIAALEPREGTTRIAVSAPGFAERLRKGDSIAVS
                     GTCLTALEMKPPIFYADLATETLARTSLGFLKPGSVVNLELPTAAGTPLGGHIVQGHV
                     DGRGELVSFDPVHSTAKPEETDWWLRVRVPEETRPYMVQKGSIAIEGISLTIATWDGD
                     IVGVAILPHTRNVTSLRVLKPGDPVNVEADVMIKLAVDQKSPAASFEVTLEYLLANGY
                     "
     gene            complement(6835..7536)
                     /locus_tag="VHE33_00970"
     CDS             complement(6835..7536)
                     /locus_tag="VHE33_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HVT96045.1"
                     /translation="MRRADRLFRIVQLLRGGRLQTARSLAEKLQVSHRTIYRDVQDLQ
                     LSGMPITGEAGVGYTLRRDLDIPPLMFDREEIAALVLGARMVEAWGGIELTEAANRAL
                     RKIEAVLPTSLRENIDDVLLYAPGYKAPPEVRRKLDTLHTAAQQRQVVLLAYTREDGQ
                     PSVRRVHPLALYFWGGTWTLAAWCELRADFRSFRVDRVQHADTIGEHFTPRAGQTLAD
                     FLRRVRSQIGDLPQL"
     gene            complement(7675..8058)
                     /locus_tag="VHE33_00975"
     CDS             complement(7675..8058)
                     /locus_tag="VHE33_00975"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT96046.1"
                     /translation="MATAEMTATSTITWFEIPAADFGRAVRFYETIFASPLRHEAAWP
                     NMAIFHCERPGVSGAVVGGEGRRPSSDGVVIYLNCDGRIDAVLGRVEKAGGGIVEAKN
                     HIPSVGWVAQIRDSEGNRIGLHAAA"
     gene            complement(8168..>9144)
                     /locus_tag="VHE33_00980"
     CDS             complement(8168..>9144)
                     /locus_tag="VHE33_00980"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HVT96047.1"
                     /translation="SRRYPGLLIKEEADNPTGSFKARGMSAAITMARHYGLKKLAAPS
                     AGNAASALAAYAAAAGIEAHIFMPRDVPMANYVEGMAYGAHVTLVDGLISDCGRMVAE
                     GAKREGWFDISSLKEPFRVEGKKTMGYELVEQLGWTYPDVVFYPTGGGVGLIGMWKAF
                     EEMEQMGWVSGQRPKMIAVQASGCAPIARAWQEDASASTLFENAATFAAGLRVPKPYG
                     DTLILEIVRESGGTVIDLPDEAILASLLDWARHEGLLLCPEGACATAAYRHLLDIGFL
                     KPEDRVVLFNTGSGLKYIDAIAEGMHLTRPGTKQYPTRTPVGGVITPQ"
BASE COUNT         1795 a         2626 c         3030 g         1693 t
ORIGIN      
        1 ttgctgctcc ggaagtcgcg cccgtggctc cattacgagg aataccaccg gtgccggcga
       61 tcgctcctct tcctcccaat cctgccgaca tcgaggtcga cgactcgcca gacacaatgg
      121 tttccgtcga cgaccaggac acgaggggtt acacctacgg cttttccgac aacggggagt
      181 cgtatgccat tgtcgaaggc tcaggcaacg atgtgacctt ctccggttcg tgggacaacc
      241 accgccgcgc ggagatcgac gccgcacgca aaggagcgaa gggcccgttc ctgtggttca
      301 cgcacgaggg gaagtcgtac atcgtcacag acccagccat catcgcgaag attcgcgcaa
      361 tgtatcagcc gatggaagaa ctggggcgca aacaggaggc gctggggaag cagcaggaag
      421 ctctgggcaa acagatggag gaattggcgc gagagcagga agcggcgggt caggtgaaga
      481 tgccggacct ttccagagaa atggctcaga tcgacgcagc gctcgcaaag ttcaaagaga
      541 acgagcatca acggaatgcc ccagccctgg cagaagctga agcgaaggtt aaagctgcgc
      601 aggataaaat gctgtctcct gagaggatgg ctgagctgca gtccgacctc gccgcagccc
      661 aggcgcagtg gaattccgag agcatgtctt cattgcagga gaggttgggt gagttgcagg
      721 cgcggctggg cgaactgcag ggcgaagccg gtgcgcgcca gggagactac ggcgcgaaga
      781 tgggtgctct gggcgcacag cagggaaagc tcggagggga acaaggccgt cttggggcag
      841 agcagggacg gatcgcgcgg caggctgatc gccaggtgcg tggcatcatc gaggattgcc
      901 tgcgcaacgg caaggcatcg cggttaccgc aggtgaagtg aagcctggca agtggagaac
      961 gagccctccg agtctggcga acatcaccgg tcaacgcgag caacggagag gtatcaaggc
     1021 gaagacacgg ctaccgtgaa gatcgaacgt aactggtaga ctcgtaaacg caatgcccgt
     1081 cattttttat ctggtcgctg cagttgtggc gtacctgctg ggttcgatcc ctttcggata
     1141 tttactggtg cggctcttcc gcaaagagga tattcgtcaa aagggaagtg gcaacatcgg
     1201 ggcgaccaac gtaatgcgct cgggctcgaa aggcctgggc gcattgacgt ttctactcga
     1261 cgtgctgaag ggtttttgcg ccgtgtggct gtgcggtgcg tacgcccttc atgccggttt
     1321 gtcgtggaat gcgcgcgtga atgcggtcgc tatcgcagcg ttgtgcgtgg tcctgggtca
     1381 catcttcaca atgtggctcg gcttcagggg gggcaaggga gtcgccaccg cgtttggcgt
     1441 ctttatcgcc ttggcgccct gggcggcgct ggcgagcgtc ggtgtttttg ccgttgtttt
     1501 tggggcgtcc cgctacgttt cgctcggctc gattgtctcg gcgctcgcat ttccgatcat
     1561 cgccgttctt ctcccgcatg cgccgcgcac gccgcttgaa aacgccgtgt tggtggtcat
     1621 tcccctgatt gtgatcctga agcatcacca gaacatcggc cggctgttga gcgggacgga
     1681 atatcgtttc ggcaagaaac aaacgacaga gcccgggaca accgccgtat gagccgcatt
     1741 gcaatcatgg gaagcggcgc ctggggcact gccctggcca ttagcctggc gcgtcgcgga
     1801 ggccaccaga tcgctctctg gtcctacgcc aaagacgtgg cagcatccat gcgcgaccgg
     1861 cgcgagaatc cacaattctt gcccgggttt cgcattcctg agtctgtcgc tgtcttcgac
     1921 aactccgccg aagcactgga aggcgcggag atcatcgtca gcgtgacgcc ttcgcagttt
     1981 gctcgtgctt cctatgaagg attcgctccc tacctgcgtc ccggtcaact gctggtgagc
     2041 gcaaccaagg gcatcgaaga cgagacttac ctccgtatga cacaggtgat cggggaggtg
     2101 cttgcatccc ggggcctgga attaccgtgt ggcgtgctga gcgggccgtc gttcgctcag
     2161 gaggtcgcgg ccggtgcccc gacggcgatc accatcgcct ctgccgatgc gaatcttgcg
     2221 tcgaaagtgc agcgcgagtt ttcgagcagc acgttgcgct tatacacgaa tgacgacgtg
     2281 gtaggcgtgg aacttggcgg ttcgctgaag aacgtcatcg cgattgctgc gggcattgtg
     2341 gccgggctgg aactgggcca caacagcaac gcggcgctca ttacgcgcgg tattgcggag
     2401 atcacgcgct tatcggtagc gtgcggcgga caacgggaga cgctggcggg actttccggg
     2461 gtgggcgacc tcgtcctgac ttgcaccggt tcgctgtcgc gaaacaggac agtgggtttt
     2521 ggtctcggac agggcagaaa gatcgatgag attttggcgg gccttgccgg ccgcgtcgcg
     2581 gagggtgtga aaaccacttc agcggcactc ggcctggcgc gccgccacgc tgtggaaatg
     2641 ccgatcgccg agcaaatgga ggccattctg ctgcatggaa aacaccccca gcgcgccatc
     2701 gaggaactga tgctgcggcc tggccgcgat gagtagagcc cccgcaggct cagttactta
     2761 ggttgcatcc accttttcac cattacgagg gtaagccctg tttgccccgc acagcggagc
     2821 gggttaccac cttcgggtga aggaaaaacc ccctttacct tcgtagactg acggagaaag
     2881 gcttggcatg caaaaggttc ccgctcgcgc ggctcggctg tcctcgccgg ggatgcgaat
     2941 tcgctggcgt gtgtttgcgc gttttctcgc gctctttgcc ggagcgactt cggtgacggt
     3001 tctggcgatg atgctggcac agcgcaccct gcgtgccgtg gtcgccggct gggccgtagt
     3061 ccttatcgaa tttgccggaa gtgctgcagc ggctctgctg ctcaccgtct ggatggaaaa
     3121 gcgcattcag cgcctcgtgg acacgcttgc gtcgaacgat gtcgcggccg ggacatcgga
     3181 cgagctgttt gccgagatca ttcccgcctg gcagcagaac gtcaaagccc tggccgacaa
     3241 agccaacgaa aaaaccgacg aggcccgcag ccattacgaa gcgctcaagg acttcatgcg
     3301 aatgatcgcc aaggccgtcg acgagcgtac cgcctatctg cgcgggcact ccgagcgagt
     3361 agccgcgtat gcggcggcca tagcctggaa gctcggactg gacgagcgtc aggtggaacg
     3421 ggtccgtctc gctgcactgc tgcacgacat aggctgcctc ggtgtcgaag actatctggt
     3481 gatgaagacc acgcccctca cgccggagga attcgagatc gtgaaggcgc acaccgtcaa
     3541 aggcgcggcc attctgcggc caatcaccat gctgcaggac ctggttccag gtgtggagct
     3601 gcatcacgaa tccctcgacg gcatgggcta tccgtatggg cttaccggag agcagattcc
     3661 gctcatcgct cgcatcgtct cagtggcaga ttcattcgac gcgatgacaa caccgcggcc
     3721 ctaccaaacg gcaatgaacg cggactacgt tctggatatc ctgacccggc tttccggtac
     3781 gcgctacgac cccgtcgtgg tcattgcgct tgccgagctg gtgaggaccg gcgcgctgga
     3841 agtgaagaat gtgcgaacac cggtctcgtt ccgcatgaga aagccggttc ctgaactcgt
     3901 ctgaaccggt gcggcctgcc ccacgcactc ccatgggttc gctacactcg atgaagatgc
     3961 gctgagggcg cagttctcat gattcagacg ttgtttggca gcctgaaaga agagcaggac
     4021 ccggaaaaac aaagcctcct tgagcgcatg cgctccgcag tgacgcgcac gcgcgaaagc
     4081 ctgggcgagc gcatcgacgg cgttctggcc ctgacgcgca cggtcgatga ggctgcgctg
     4141 gaagagctgg agatgagcct gatcgcgtcc gacatcggcg tgaatacggc ggccgaaata
     4201 acaaaccatc tgcgcgagcg cgcgaaacgc caacaaattc aggatggagc agagctgaag
     4261 gggctgctcc gggaggagct gcaggcgatc ctggatgaaa atgccccccc ggcaaaatcg
     4321 gtcccgcgag cgcccgaagt gatcatgatg gtgggcgtga acggcacagg caagacgacg
     4381 accagcggta agctggcggc cttctaccgc tcccagggca aaaccgcgct cctgtgcgcg
     4441 gcggacacgt ttcgggcggc ggccatcgag cagctcgagg tgtggggcgc gcgcagtggc
     4501 gtggagatga tcaaaacaaa acagggtggg gatccctccg cagtactctt cgacgcgtta
     4561 caggcagccc agacgcgcca gaccgatgtg gtgattgtcg acacggctgg gcgtttgcat
     4621 actaagacag gtttgatggc cgagctggac aagatgcggc gcaccgcgca gaggatcatt
     4681 tccgacgcgc cgcacgaggt gctgctggtg ctcgatgcaa caacgggaca aaacggtctc
     4741 cagcaggcgc gcctctttac cgagtcggcc ggagtgacag gcattgtgct gaccaagctg
     4801 gacggaacgg cgaaaggcgg catcgccgtg gcgattgcac gcgaaatgaa gttgccggtg
     4861 cggtttgttg gaattgggga ggcgctggaa gatctgctgc cttttgacag cggagcgttt
     4921 gtgaattcgt tgctggagaa ctgagcgagg cgtggcctcg cgatttcgaa agggtagtgt
     4981 ggctcaggga ttcgatcctc tgcctgttga cgagggctgg atgaggcgag cgctggaact
     5041 ggcgcagcac tcgatcggct tggcttctcc aaatcctatc gtcggctgtg tgctggtgaa
     5101 agatgacgtg atcgtcggcg tcggccggca cgaatacgac aagaaagatc acgccgagat
     5161 cgtggccctg aaaaacgcgg gcggttctgc acgcggagca acagcctacg taacgctgga
     5221 gccatgctgc cacaccggac gcaccggccc ctgtacgaag gcgctgattg aagctggtgt
     5281 cgcgcgcgtc gttgcagcga cggcggatcc aaatccagcg gtgagtggga aggggcttga
     5341 gcaactgcag gcggccggga tcgaagttgc tgtcggtgtg ctgcagtgtg gagcgcgcgc
     5401 attgaacgat ggcttcgcga agcacattcg cacgggtttg ccgttcgtga ccctgaaggc
     5461 tggtttgtcg ctggacggtc gcattgcgcc ggcgccgggc agcgccccgc acggagcgcc
     5521 cgttttcctt acaggcgaca aggcccggga gcaggtacag gagatgcgcc acgcggcgga
     5581 cgctgtcatt accgggatca acacaatttt gcaggacgat ccccaactca ctgatcgatc
     5641 cggaatgccg cggcggcgac cgctgctgcg cgtcgtattg gattccgggt tgcggatccc
     5701 gttggattct aagctcgtgc gcacggcgca ggacgatctg ctgatcttct gtggtgtggc
     5761 caccacccag cggcagcaaa ccctggaggc gcttggtgtt cgggtggagc gaattccgac
     5821 agctgaagcg gtttctgatc agtttgcaac cgggatgccg acgcacggac gtaccgcgcg
     5881 ccgcaccgga gtctcgctga aaacggtgat gcaccgcttg ggcgaaatgg ggattctctc
     5941 ggctttgctg gaagccggcg gacaattgaa tgcgtcggcg ctgaacggtg gatacgtcga
     6001 taagctcacg ctgttttatg cgccggtctt cctcggctcc gacggagttc ctcttctgca
     6061 ggagggaatg ttaaagcccc tcctgccgac tccaccgctc atagaacgcg tcggcaacga
     6121 tgtacgaatc gacgcctggt tgcgtaaccc ctggggggaa gcctgaatca tgttcacggg
     6181 gatcattgaa gctacaggag agatagcggc gctggagccg cgcgagggga caacgcgcat
     6241 tgccgtctcc gcgccgggct ttgctgaacg actccgcaag ggtgacagca ttgcggtaag
     6301 tggaacctgc ctgacggccc tcgaaatgaa gccaccgatc ttctatgccg atctggcgac
     6361 ggagacgctg gcaaggacgt cacttggatt cctcaagccg ggttccgtcg tgaacctgga
     6421 gttgcccact gcggccggca ccccactcgg tggccacatc gtgcagggac acgtcgatgg
     6481 ccgtggagag ttggtttcgt tcgatccagt gcactctacc gcgaaaccgg aagagactga
     6541 ctggtggctg cgtgtacggg tgccggagga gacgcggccg tacatggttc aaaagggatc
     6601 cattgcgatc gaaggcatca gcctgacgat tgccacgtgg gacggggaca tcgtcggcgt
     6661 cgccattctg ccgcacacgc ggaacgtgac aagcctccgc gtgctcaagc cgggtgatcc
     6721 ggtcaacgtc gaggcagacg tgatgatcaa gctggccgtg gaccagaaaa gtcccgcagc
     6781 cagctttgag gtaacactcg aatatcttct tgcaaatggc tactaggcta tcgtttacag
     6841 ctgaggtagg tcgccgatct ggcttcgcac gcgtcgcagg aagtccgcca gcgtctgacc
     6901 ggctcgcggc gtgaagtgct caccgatcgt gtcggcatgc tgaacacgat cgacacgaaa
     6961 actgcggaaa tcagctcgga gctcacacca ggcggccaga gtccaggtgc ctccccagaa
     7021 atagagcgcc agtggatgaa cgcggcggac gctgggttgt ccatcttcac gggtatacgc
     7081 aagcagaacg acctgccgtt gttgtgcggc cgtatgcagc gtgtcgagct tgcgtcggac
     7141 ttcgggcggc gccttgtagc caggcgcgta gagcagcaca tcgtcgatgt tctcgcgaag
     7201 actggtgggg agaaccgctt cgatcttgcg cagggcgcgg ttggctgctt cggttagctc
     7261 aatgccgccc caggcctcga ccattcgagc gccaaggacc agagcggcga tttcttcgcg
     7321 atcgaacatg agcggtggta tatcgagatc gcggcggagc gtgtagccaa cgcccgcttc
     7381 gccggtgatg ggcatgccgg aaagctgcag gtcctgtaca tcgcgataga tggtgcgatg
     7441 agagacctgc agtttctcag cgaggctgcg cgctgtttgc aggcggccgc cgcgcagcaa
     7501 ctgcacgatg cggaagaggc gatcggctcg gcgcataccg gcattgtagc gagcaaacta
     7561 cgagcgaaag ccgagtcggc gcgagcgagg cagcgagcag tcagggtccc gctcgcagcc
     7621 tgctcggtgt ttcctcgccc ctgcgcccgt cttcggcaga gctacacccg aggatcaggc
     7681 agcggcgtgc agtccgatgc ggttgccctc tgaatcgcgg atctgcgcca cccagccgac
     7741 gctgggaatg tggttcttgg cctcaacgat gccgccgccc gccttttcca cccggccgag
     7801 gaccgcatcg atgcggccgt cgcagttcag gtagatgacg acgccatcgg aagatgggcg
     7861 gcgaccttct cctccgacga cagcgccgga gacgccagga cgctcgcaat ggaagatcgc
     7921 catattaggc caggcggcct cgtggcgcag ggggctggcg aagatggttt cgtagaagcg
     7981 gacggcgcgg ccgaagtcgg cggcgggaat ctcgaaccag gtgatggtcg aggtggcggt
     8041 catttcagca gtggccattg gatttcctct cggattcgaa gtcgactacg agaggagcgt
     8101 aggacacccc tgctgacagc gttctgtcag tagagaacag cggagcgaac atagtgacag
     8161 cattccgcta ctgaggcgtg atgacgcctc caacgggcgt cctcgtcggg tattgcttgg
     8221 taccagggcg cgtgaggtgc atgccctcgg cgatggcgtc gatgtatttg agaccggaac
     8281 cggtattgaa caggacaacg cgatcctcgg gcttgaggaa gccgatatcc agcaaatggc
     8341 ggtaggcggc ggtcgcgcag gcgccctccg gacacagcag cagtccctcg tgacgggccc
     8401 agtcgagcag ggaagccagg atggcctcgt caggcagatc aatcaccgtg cctccggact
     8461 cgcgcacgat ttcgaggatg agcgtgtcgc catacggctt ggggacgcgg agaccggccg
     8521 caaaggtggc agcgttttcg aacagggtcg acgccgacgc gtcttcctgc caggcgcggg
     8581 cgatgggagc acagccggag gcctggacgg cgatcatctt cggacgctgg cctgaaaccc
     8641 aacccatctg ctccatctcc tcgaacgctt tccacatccc gatcaggccg acgccgccgc
     8701 cggtcgggta gaagacgacg tcggggtagg tccagcccag ttgctcgacc agttcgtagc
     8761 ccatggtctt cttaccttcg acgcgaaagg gctccttgag gctggaaata tcgaaccagc
     8821 cctcgcgttt ggcgccctca gcaaccatgc ggccgcagtc cgagataagg ccgtcgacca
     8881 gcgtaacgtg cgcaccgtag gccataccct cgacgtagtt agccatggga acgtcgcggg
     8941 gcatgaaaat gtgtgcctca atgccggcgg cggcggcgta ggcggccaga gcgctggcgg
     9001 cgttgccggc cgaaggggcg gcgagcttct tcagtccgta gtggcgggcc atggtgatgg
     9061 cggcggacat accgcgggcc ttgaagctgc cggtgggatt gtcagcttcc tccttgatca
     9121 ggaggccggg gtagcgacgg ctgg
//
LOCUS       DASZYN010000033         5397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_771824, whole genome shotgun sequence.
ACCESSION   DASZYN010000033 DASZYN010000000
VERSION     DASZYN010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771824"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1776)
                     /locus_tag="VHE33_00985"
     CDS             complement(7..1776)
                     /locus_tag="VHE33_00985"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HVT96048.1"
                     /translation="MQHRKFLILGMALLLGVPASQAKEPRASSGLPLTPQQQRLVQQA
                     IAREKVVVRQIQKSTPVVQSYIQDVRADQKLYQVPVSDQYMIGRVDFGKAFTEAQYEQ
                     ADRTEHHGWFAGSFHFMSELTKAFHLENSPTGFMAMMFVDPTGFDQQHYNFSFVRREF
                     LGTVRTWVFDVQPKEKGPGRFFGRIWVEDQDGNVVRFNGTYVNNPNDEADHYFHFDSW
                     RMNVQPGVWAPAAVYIEDTLHNGANHATSFKGQTTYWGYSLKLPTKESDSESMEIENA
                     QDQSDNSTDVSPLQAEREWISQAENNVLDRLTQAGLVAPPSDFDKQLEQVATNIIIGS
                     NLQLPSDIHCRVILTTPLESLAVGNTILLSKGLIDTLPDENALAAVIAFQLAHIVTGH
                     HIDTRYAFNDRLLFPDTATFRRITMNHSNPDNEEAAKRAVELFSHSIYHDKAGSVGLF
                     FQQLEAREKDLPSLFTPRLGDSLVRGDGQPWLAGLTQGAPKLDMDNLQQVAALPLNSR
                     LKIDPWDDKVFMLNARPPVLLNARDKMPLELTPVFFRLQRYSDAPAVPANATPAASVP
                     PSADAAAQQPSASAQARPQAGPQ"
     gene            complement(1865..2941)
                     /locus_tag="VHE33_00990"
     CDS             complement(1865..2941)
                     /locus_tag="VHE33_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HVT96049.1"
                     /translation="MAIDNSVKRATFGLLPEPEGRMGSFGMSMVINLTVGALMLLLAI
                     AQVHQVQEHRYQTTQLIFPVEQPKPYVPPVPKIKLPPPPQIAKLEQPKIEMPRPVPPP
                     PKVAEIKLPTPMMPKIEAAPPKRFAPPPQPKLGAFKSETPTTVANNMTAPTPKTGGFG
                     DPAGVKPNPTATRTATIAAVGAFNAAPGTASGAGAARQGAVRGADFGSGVASGVPGGK
                     DRNATVASAGFGNGVLGGTGSKDSHGTVARGSFGDNMYGSAPAPAVHAQAPQTTPIVV
                     LAKPLPVYTSEARQLKIEGDVTLEVRFAASGQVQVLRVVNGLGHGLDEQAKLAAERIR
                     FKPATRDGQAVDQVSIIHVTFQMA"
     gene            3463..4449
                     /gene="galE"
                     /locus_tag="VHE33_00995"
     CDS             3463..4449
                     /gene="galE"
                     /locus_tag="VHE33_00995"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HVT96050.1"
                     /translation="MRIVRILVTGGAGYIGGMVSLALLQRGHTVVVFDNLCHSTRELL
                     PPGADFVEGDLADRPQLEELLASGFDGVMHFAALIEAGESMRQPERYFRNNTASTLSL
                     LEAMISTGVHRIVFSSTAAVYGEPDVTPIREDATLRPTNAYGESKLLVEKMLNWMYRV
                     HGFRYASLRYFNVAGAMGARGEAHEPESHIIPLVLDVALGRRDRIKIYGDDYPTPDGT
                     CIRDYIHVEDLAEAHLLAFEALASRGPLIYNLGNGSGFSVRQVIESARRVTGHPIPVD
                     VVPRRPGDPAVLIASSEKIAQELGWRPRHTDLDEIIASAWQWHQVRYSTKDR"
BASE COUNT         1033 a         1779 c         1498 g         1087 t
ORIGIN      
        1 tgcagattac tgtggcccgg cctgcggccg tgcctgcgcg cttgccgacg gctgctgagc
       61 cgccgcatct gccgaaggcg gcacactggc ggccggcgtt gcgtttgccg gaaccgcggg
      121 tgcgtcggaa taacgctgca gacggaagaa caccggcgtc agctccagcg gcatcttgtc
      181 ccgcgcgttc aacagcaccg ggggccgcgc attcagcatg aaaactttgt cgtcccaggg
      241 atcgatcttc agccggctgt tcagcggaag cgccgccacc tgctgcagat tgtccatgtc
      301 cagcttcggc gcgccctggg tcaggcccgc cagccacggc tgcccgtcgc cacggaccag
      361 cgagtcaccc agccgcggcg tgaacagcga gggcagatct ttctcccgcg cctccagttg
      421 ctggaagaac aagcccacgc tgcccgcctt gtcgtgatag atcgagtggc tgaacagctc
      481 caccgccctc ttggcagctt cctcgttatc cggattcgag tggttcatcg tgatccgccg
      541 gaacgtcgcc gtgtccggga acagaagccg gtcgttgaag gcataccgcg tgtcgatgtg
      601 gtgtccggtg acgatgtgtg cgagctggaa ggcgatcacc gcggccagcg cgttctcgtc
      661 cggcagtgta tcgatcaggc ccttgctcag caggatggta ttccccaccg ccagcgactc
      721 cagcggagtc gtcaggatca cgcggcagtg aatatcgctg ggcagctgca gattgctgcc
      781 gatgatgatg ttggtcgcca cctgctccag ctgcttgtcg aagtcgctgg gaggcgccac
      841 cagcccggcc tgagtcagcc ggtccagcac gttattttcc gcctgcgaaa tccattcccg
      901 ctccgcctgc aacgggctca catcggtcga gttgtcgctc tggtcctggg cgttctcgat
      961 ttccatcgac tccgaatccg actctttggt cggcagcttc agagaatagc cccagtaggt
     1021 cgtctgaccc ttgaagctgg tcgcatggtt cgcgccgttg tgcagcgtgt cttcgatgta
     1081 caccgccgcc ggcgcccaca cgcccggctg cacgttcata cgccagctgt cgaaatggaa
     1141 gtaatgatcg gcttcgtcgt tcgggttgtt gacgtaggtg ccgttgaagc gcaccacgtt
     1201 cccgtcctga tcctcaaccc agatgcgccc gaagaagcgc cccggccctt tttccttcgg
     1261 ctgcacatcg aaaacccacg tccgcaccgt ccccagaaat tcgcggcgca caaacgaaaa
     1321 attgtagtgc tgctgatcga atcccgtcgg atcgacgaac atcatcgcca tgaatccggt
     1381 cggcgaattc tccagatgga acgccttggt cagctctgac atgaaatgga aggagcctgc
     1441 aaaccagcca tggtgctccg tccgatccgc ctgctcgtac tgggcctcgg tgaaggcctt
     1501 gccgaaatcc acgcgcccga tcatgtactg atcggaaacc ggaacctggt agagcttctg
     1561 gtccgcacgc acatcctgga tgtacgactg cacgaccggc gtgctcttct ggatttgccg
     1621 gacaacgact ttttcgcgcg cgatcgcctg ctgcaccaga cgctgctgtt ggggggtaag
     1681 gggcagtccc gacgaagcac ggggttcttt cgcctgggac gccggaacgc ccagcaacaa
     1741 tgccattccc agaatcaaaa acttgcgatg ttgcatttcg ttatgactcc tgaatacgtg
     1801 ccttgtgttc ggcctttgca agaagcgcgg gcgatgccgg cgcctcgttc ccagctgcgc
     1861 ctcatcaggc catctggaat gtcacatgga tgatactcac ctgatccacc gcctgcccat
     1921 cgcgggtcgc aggcttaaag cgaatccgct ccgccgccag ctttgcctgt tcgtccagtc
     1981 cgtgccccag tccgttaacc acgcgcagca cctgcacctg gccgctcgct gcgaagcgca
     2041 cttcgagggt cacatcgcct tcaattttga gttgccgcgc ctcgctcgtg taaacaggca
     2101 aaggcttggc caaaaccaca atcggcgtcg tctgcggcgc ctgcgcatgc accgccggag
     2161 ccggcgccga tccgtacatg ttgtcgccaa aactgccccg cgcgaccgta ccgtggctgt
     2221 ccttcgatcc cgttccaccc agcacgccgt tgccgaatcc tgccgatgcg accgtggcgt
     2281 ttctatcctt gccgccggga acgccgctcg ccacacctga accaaaatcc gctccgcgca
     2341 ctgcgccctg gcgagctgct ccggctcctg aagcggtacc tggcgctgcg ttgaaagctc
     2401 caaccgctgc gatcgtcgcc gtccgcgttg ccgtcggatt cggcttcacg cccgccggat
     2461 ccccaaaacc gccggtcttc ggcgtcggag cggtcatgtt gttcgccacc gtcgtcggcg
     2521 tctcgctctt gaacgcgccc agcttcggct gcggcggagg cgcaaaccgc ttaggcggcg
     2581 cggcctcaat cttcggcatc atcggagtcg gcagcttaat ctccgcaacc ttgggtggcg
     2641 gcggcaccgg cctcggcatc tcgatcttcg gctgctccag cttcgcgatc tgcggcggag
     2701 gcggcagctt gatcttagga acaggcggca catacggctt cggctgttca accgggaaaa
     2761 tcaactgcgt cgtctggtaa cgatgctcct gcacctgatg aacctgcgcg atcgccagca
     2821 gcagcatcaa agcacccacg gtcagattga tgaccatgct catgccaaac gagcccatcc
     2881 ggccttctgg ctcggggagc aacccgaacg ttgcgcgctt aacggagttg tcgattgcca
     2941 ttgccctgta tctccaacct cacctgagaa aagaagttgc gactttcagt ctgtgcatct
     3001 ttccgatttg gcatgcactt cgatgaccag tgaaccgaac cggcggtggc ccgacttcag
     3061 acaagccctg gcgcaagact gcgggatacg tgctcccgca aaactcgccc tgcttgtggg
     3121 gttaccattg aaatcttcct acgggtactt ctggacgcgc aagaggtgac ttgagagtac
     3181 tccgaggaat tacctccttc agtcacacaa acgaggcaga atcgtgccca ttttgggtct
     3241 tgctaccaat gaacgacgcg aacaggggcg tactcgttca ctttctctac ttccctgcgc
     3301 tgcatatctg cagggctcac cagcggctgt ccctcaggat cgtcggacca aatccaggag
     3361 cgaggagctt cccgccgcgg ttaccacagt cgaagcgccc tctcgatgaa ttataaagag
     3421 tctgacgcag ccgggacgta aacagcataa aggattcagg gggtgaggat cgtgcgaatt
     3481 ctggtcactg gtggagctgg gtacattggt gggatggtct ccctcgcgct tctccagcgg
     3541 ggtcatacag ttgtggtctt cgacaacctg tgccattcca cacgcgagct cctcccgccc
     3601 ggcgccgatt tcgtcgaggg cgacctggcc gaccggcccc agctcgaaga gcttctcgcg
     3661 tccggattcg atggggtgat gcattttgcg gcactcatcg aagccggcga aagcatgcgc
     3721 cagccggaac gctacttccg caacaacacc gcttcgaccc tgagcctcct cgaagccatg
     3781 atttccacag gcgtccacag gattgtcttc tcttccaccg ccgccgttta cggcgagccc
     3841 gacgtaaccc cgatccgcga agatgcgact ctccggccca cgaatgccta cggcgaatcc
     3901 aagctcctgg tggaaaaaat gctcaactgg atgtatcgag tacatggttt tcgatacgcc
     3961 agcttgcgct atttcaatgt cgccggggcc atgggcgccc gcggcgaggc gcacgagccc
     4021 gaatcccaca tcattcccct ggttcttgat gtcgctttag gccgtcgtga ccgaatcaaa
     4081 atctacggcg acgattaccc cacccccgac ggcacctgca tccgcgatta catccatgtt
     4141 gaggacctcg ccgaggcgca tctgctcgcc ttcgaggcgc ttgcctcgcg agggccgctc
     4201 atctacaacc tcggcaacgg atccggcttc tcggtccgcc aggtcatcga atcggcgcgc
     4261 cgggtcaccg gccatcccat tcccgtcgac gtggtgcccc gccggccggg agatcccgcc
     4321 gtcctgatcg ccagttcgga gaagatcgcc caggaactgg gctggaggcc ccgtcacacg
     4381 gatctggacg agatcatcgc cagcgcctgg caatggcacc aggtgcggta cagcacgaaa
     4441 gacagataag agccgaaacg gcgcgatctc aagaggctgc cactctgagc cccgcacagg
     4501 cgaaggacct ttctcgttcg gataacttcc ggaaatccgg tctctcggca ggcgtcctct
     4561 gccgcgagca cctaccgcat tgatgcagcc tgcttttgtg ccgtagtaat caggacatta
     4621 gccgtaataa tgcccacggc taccattccc aagcttctgc caagttcggc gggcacgacg
     4681 gcgagagaga acgccaagct agccgtcgcc aggtacagcg caaatatgcc cattccgtag
     4741 tacaaaccgg gcatggacgg atcttgttcc cgttcagtgg gcgacagatc acgccgggga
     4801 ggtgctggag acttgatcca tcgctcatgc atccgaatct ggctcacaat cagcaacagc
     4861 gcgcctacga ccagaacccg cgtcactata cccatcgcag aaagggggac ttccattagg
     4921 gcccacacgt acacaatcga agtgagcgtg acaccgagcc aaactccaat caagggccat
     4981 cgtctacgca ttcgatgaga gtagattcct cgcctcaatg agtccatccc acgcctgagc
     5041 caggccatcg caatcccaat gcagcgcatt atttctgtaa tgtcggcctt ccggaacatt
     5101 ggccccctcc cgggcccctt gtggattcct gtcaaccccc aaccctcacg gtacaaacgt
     5161 gttccccctc attccaggac acttacccgc cgatctgccc cgaaaacgtt ggcacgattt
     5221 ctccttcgaa ctgctattct gagaataggc agacagaatg aaaagcccgc cctcatcagg
     5281 gcgggctttc tgtttctgcc tcatcgtcgg atccggctca ggcccgatcc catggattcg
     5341 ctctttgaaa acctgacgtc cgcggcggtc ccgattcacg gtcgcttcga taaccaa
//
LOCUS       DASZYN010000034         3310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1029606, whole genome shotgun sequence.
ACCESSION   DASZYN010000034 DASZYN010000000
VERSION     DASZYN010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029606"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VHE33_01000"
     CDS             complement(<1..262)
                     /locus_tag="VHE33_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVT96051.1"
                     /translation="MTAIIEADRLTIRFGQLTAVNDVSLEVEKGEIFGFLGPNGSGKT
                     TLIKALCGLLAPSEGTGRILGMDIRKDAPEVRRHVGYMSQKFG"
     gene            complement(259..1386)
                     /locus_tag="VHE33_01005"
     CDS             complement(259..1386)
                     /locus_tag="VHE33_01005"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HVT96052.1"
                     /translation="MKRRIVLIGAVALVVIAGTWIVLAWTGWRARNDYSGTVETREIQ
                     IGSKVGGRVTEVRVEEGQSAKAGTVLVRFECDELKAQRAQAQASVEQAEADLERMVRG
                     NRPEEIAQAVASAHAQQAAFEEAQNGPRKQEIEQAKADLAAAQADATNAESFYRRMDE
                     LEKSDIISKQQFDDAKDKRDAAQQRAESARQRLALLQAGTRAEDVNAAEARYRQAQAA
                     AVLAKKGFRREDIEAARGKLAQAKGQVAELDARLREAELTAPADAVVETVSVRPGDLV
                     PAGHIVMTMLESSQLWVKVYVPETDLAHVHLGQRSGVKVDSLGRTFAGHVGMIASEAE
                     FLPRNVQTKSDREHQVFAVKVYVDDAQQVLKSGMSATVRLE"
     gene            complement(1383..2000)
                     /locus_tag="VHE33_01010"
     CDS             complement(1383..2000)
                     /locus_tag="VHE33_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT96053.1"
                     /translation="MNPTTTPGRAHPTTLHGRRKQQTRGEIVRAAFELFGQHGYESVS
                     MDIVAEAAGVSRATLFNYFPQKELILREIALARVAKLKAILGDFAANGKKVRYDDVVD
                     LIVRLTVENARIVGGAKKLFLEAMFRQASQGVLLTARAQAVQALTTSVGRFVRGEKKA
                     RRVAETAFAIYMATMLEWVMRDEVPQKWLVERMRERLLVLEEGVA"
     gene            2232..2561
                     /locus_tag="VHE33_01015"
     CDS             2232..2561
                     /locus_tag="VHE33_01015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVT96054.1"
                     /translation="MPEKPGDLVQGTLEMLVLKTLALEPMHGYGIALRLEQVSHGVFR
                     INPGSLLPALSRLERAGRIKAEWRASENNRRARYYQLTPRGHKALEDERALWDRQIAA
                     INRILEA"
     gene            2588..>3310
                     /locus_tag="VHE33_01020"
     CDS             2588..>3310
                     /locus_tag="VHE33_01020"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT96055.1"
                     /translation="MFSSLIAGIRALFRRDRRNADIQREVDSFLLASIDDKMRSGMSR
                     DQAVRRARAEIGSSESVRHKVWSAGWESTAESLLHDIRFALRQLRKTPGFTVTAVLTL
                     TLGIGANAAIFTLVNAVLLRNLPVADPATLVRLGNTNQCCVGSGFIPDDDSYSSFSTN
                     TWQEMRKDLPEFQELAAMESGFTYRPVTVRRDGSQEPARSVMGEFVSGNYFRTFGLTP
                     ASGRLFADADDNSGAPMVAVMSY"
BASE COUNT          543 a         1262 c          852 g          653 t
ORIGIN      
        1 agccgaactt ttgcgacatg taaccgacat ggcgacgtac ttccggagca tccttccgga
       61 tatccatgcc cagaatccgc cccgtgccct cgctcggcgc gagcagcccg cataatgcct
      121 tgatcagcgt cgtcttcccg gatccattgg ggccgaggaa cccgaagatc tcccccttct
      181 cgacctccag actcacatcg ttcaccgcag tgagctgccc gaagcgaatc gtcaggcgat
      241 cggcctcgat gatcgctgtc attcgagcct caccgtcgcc gacattcccg acttcagcac
      301 ctgctgcgca tcgtccacgt agaccttcac cgcaaacacc tggtgttcgc ggtcgctctt
      361 cgtctgcacg ttgcgcggca ggaactccgc ctcgctcgca atcatcccca catgccccgc
      421 gaaggtgcgc cccaggctgt ccacctttac ccccgaccgc tgccccagat gcacatgggc
      481 aagatcggtc tccggcacat acactttcac ccacagctgc gacgactcca gcattgtcat
      541 cacgatgtgc cccgccggca ccagatcccc aggccgcacg ctgactgtct ccaccacggc
      601 atcggcggga gccgtcagct ccgcctcccg cagccgtgca tcgagttccg caacctggcc
      661 cttcgcctgc gccagtttgc cgcgtgccgc ctcgatgtcc tctctgcgaa atcctttctt
      721 cgccagcacc gccgcggcct gcgcctgccg gtagcgcgct tccgccgcgt tcacatcttc
      781 ggcccgcgtt cccgcctgca gcagcgccag ccgctggcgg gcgctttccg ctctttgctg
      841 tgccgcatcg cgcttatctt tcgcatcgtc gaactgctgc tttgagatga tgtcgctctt
      901 ctccagttcg tccatgcgtc gataaaagct ctcggcgttc gtcgcatcgg cctgcgccgc
      961 tgccaaatcc gccttcgcct gttcgatttc ctgcttccgc ggtccgttct gcgcctcttc
     1021 gaacgcggcc tgttgcgcgt gcgccgaagc gaccgcctgg gcaatctcct ccggccgatt
     1081 gccgcggacc attcgctcca gatcggcctc cgcctgctct acggatgcct gcgcctgtgc
     1141 ccgctgcgct ttgagttcgt cgcattcgaa gcgcaccagc acggttccgg cctttgcgga
     1201 ctggccctct tcgacgcgca cctccgtcac gcgcccaccg accttcgatc cgatctgaat
     1261 ctcgcgcgtt tccacggtgc ccgagtagtc gttgcgcgcg cgccatcccg tccacgccag
     1321 cacgatccag gttcccgcaa tcaccaccag cgccactgcg ccgatcagca ctatccgccg
     1381 cttcatgcga ctccctcctc cagcaccagc agccgctccc gcatcctctc caccagccac
     1441 ttctgcggga cctcgtcccg catcacccac tcgagcatcg tcgccatgta gatcgcgaag
     1501 gccgtctccg ccacccggcg cgccttcttc tctccgcgca caaaacggcc gacggacgtc
     1561 gtcagcgcct gcaccgcctg ggctcgcgcc gttagcagaa ctccctggga ggcctgccga
     1621 aacatcgctt cgagaaacag cttctttgcg ccgccgacaa tgcgcgcatt ctcgaccgtg
     1681 agccgcacga tcagatccac cacgtcgtcg tagcgaactt ttttgccatt cgccgcaaaa
     1741 tccccgagga tcgccttcag cttcgccacc cgcgccaggg caatctcgcg caggatcagc
     1801 tctttttgcg ggaagtagtt gaacagggta gcgcgagaaa ctccagccgc ttcggccacg
     1861 atgtccatcg agacgctctc gtacccgtgc tgtccgaaca gctcaaacgc cgctcggacg
     1921 atctctccgc gggtctgctg ctttctgcgg ccgtgcaggg tcgtcgggtg tgctcttccg
     1981 ggagtcgtgg ttggattcat cgtcacaact tagactatag tctaatacgt ggattgaagg
     2041 ccagatttcc cgcggctggg ccggtacgaa cgtaaccatc cgaacgattc cggtgattcc
     2101 ccctctttgt gccctcttct ccgaaacgcg gcggcgggtg acctcctttc ggtcatgctc
     2161 cgtcactctc ctcttgacaa tcgagagaag ctcgctgtac ctcttgacca tcaagaggag
     2221 aactgcgctc catgcccgaa aagcccggcg acctcgtcca gggaaccctc gaaatgctcg
     2281 tcctcaagac ccttgccctc gagccgatgc acggctacgg catcgccctg cgtctggagc
     2341 aggttagtca cggggtcttc cgcatcaatc ctggctcgct gctccctgcc ctgagccgcc
     2401 tggaacgcgc cggccggatc aaggccgaat ggcgcgcttc ggaaaacaac cgccgcgccc
     2461 gctactacca gctcaccccg cgcggccaca aagccctcga agacgagcgc gccctctggg
     2521 atcgccagat cgcggccatc aaccgcatcc tcgaagcctg accagagcac agcaaggaga
     2581 aaagcccatg ttttccagtc tgatcgccgg cattcgcgct ctctttcgcc gcgaccgccg
     2641 caacgccgac atccagcgcg aagtggacag cttcctgctg gcctccattg acgacaagat
     2701 gcgcagcggc atgtcccgcg atcaggccgt gcgccgcgcc cgcgccgaaa tcggcagctc
     2761 ggaatccgtc cgccataagg tctggtccgc cggctgggag tccaccgccg aatctcttct
     2821 gcatgacatc cgcttcgcgc tacgccagct ccgcaaaacc cccggcttca ccgtcaccgc
     2881 ggtcctcacg ctcacgctcg gcatcggcgc caacgccgcc attttcacgc tggtcaatgc
     2941 cgtgctcctg cgcaacctcc cggttgccga tcccgccacc ctcgtgcgcc tcggcaacac
     3001 caaccagtgc tgcgtcggca gcggttttat tcccgatgac gactcctact cttccttctc
     3061 caccaacacc tggcaggaga tgcgcaaaga ccttcctgaa ttccaggagc tggccgccat
     3121 ggaatccggg ttcacttacc gccccgtcac cgtgcgccgc gacggcagtc aggagccggc
     3181 ccgttccgtc atgggcgagt tcgtctccgg caactacttc cgcaccttcg gcctgactcc
     3241 ggcatccggc cgcctgtttg ccgacgccga tgacaactcc ggcgctccta tggtcgccgt
     3301 catgagctac
//
LOCUS       DASZYN010000035         5309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_964440, whole genome shotgun sequence.
ACCESSION   DASZYN010000035 DASZYN010000000
VERSION     DASZYN010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964440"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VHE33_01025"
     CDS             <1..188
                     /locus_tag="VHE33_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96056.1"
                     /translation="VHDFANLGCGERGFHGILQSSEARSLYHSPEAERNHDLAGYWTM
                     NPTPFKYPTMKEMGLNP"
     gene            complement(185..1222)
                     /locus_tag="VHE33_01030"
     CDS             complement(185..1222)
                     /locus_tag="VHE33_01030"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HVT96057.1"
                     /translation="MRIGMLTGGGDCPGLNAVIHAVVRKGINHYGDEFVGFLEGWRGV
                     LENKAVSLTLEKVDGILTEGGTILRTSRTNVRKVEGGIEKCVQVLKANKIDALIALGG
                     DDTQSVTNALIQAGVPGVGVPKTIDNDLNGTDVCFGFDTAVMIATEAVDRLHTTARSH
                     ERVIVCEVMGRDAGWIALTAGIAGNAHVILVPEKPIDLDHVCAILKKNYAGDKKYGIV
                     VVAEGAKLPEIGGQATKGTKVDSFGHAQLSGIGEHLAQEIEKKTGFETRSVNLGHTQR
                     GGTPTAYDRMLATRYGLAAIDLVHQGKFGRLVVLHGTKIEDIPLAEAISKNRTLDDSF
                     LSLIGALEPKL"
     gene            1417..2268
                     /locus_tag="VHE33_01035"
     CDS             1417..2268
                     /locus_tag="VHE33_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HVT96058.1"
                     /translation="MSLLKVIVLAVIQGLAELLPVSSSAHVVVAEKLMGMDPSAPAMT
                     LLLVMLHTGTMFAVIVYFWKQWKQTFFSDGEAFKRFAWQAIIATFCTGVVGEVVIKII
                     EHTLFRGAPKAQIEDLFSHLEIVAPALLAAGILILVAGLKEGRRPDAQEAPEIGFGQA
                     GIIGAVQGLCLPFRGFSRSGATISVGILQGIKKVRVEAFSFALAVIITPLAIGREVLR
                     LRHADSTGFAHAAAPSLLGMVFSFAAGLLALKWLSAWLERGRWYLFGIYCIVASIVVF
                     ALHAKGY"
     gene            2342..2608
                     /locus_tag="VHE33_01040"
     CDS             2342..2608
                     /locus_tag="VHE33_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96059.1"
                     /translation="MWLRKLRIDRIAPNGEAIPCPIAWIDNFAMRNFTNDAIFDDTLP
                     AGDGVLEAGQRVPLDRLQAAMDDWFRRKGYIKPDEMLRVEEAPR"
     gene            complement(2615..3574)
                     /locus_tag="VHE33_01045"
     CDS             complement(2615..3574)
                     /locus_tag="VHE33_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96060.1"
                     /translation="MRMVLLLLIVLWEWPAALAQVVSYPPFSEAIAHARSLEAGALAS
                     DVPHHVHYDLKLYSHTGKRTKGTWDIWRDPLRFTRTDIVAGDFHYTQIEDLTHTRQWR
                     HFDGLMPLKVHDLLENYRQPDFAVSFFAIPQPNHYVHFQQVEGSPFTCTNEQMQMRVC
                     FDPFAHVLAFAEMFNQTVTWEDWQPVGTHMVPRRFRIYDAGRIMVEASGSAEVVKAFP
                     ADLFTIPANEPDMGEPEDNNAVPHKVIGVKPLQLDPLYGNLLVKVEVNADGLVHKVHL
                     IDADDDDLIHDGVEYAKDLTFAAQKAPFEQYIYLRYALGEPVE"
     gene            3756..4400
                     /locus_tag="VHE33_01050"
     CDS             3756..4400
                     /locus_tag="VHE33_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVT96061.1"
                     /translation="MLGLATGPVCLASCGPVVLPWMLVQPQGIRVHARQMTFFLGARL
                     AGYLLFASLIWLAGSAIAQSWGEHTWMYGAVQILLAGGLLFYAAGWPRAHCAVAQSEP
                     PGLVQIATSPVEPPQSRPTGAMALGFLTGINLCPPFLIAGVRAATLPQIHDALFFFAV
                     FFVGTAVWFIPFLSLSVVRRTPSFLTVARMVAVLLACWYGFSGASLLIQKVIYG"
     gene            4393..>5309
                     /locus_tag="VHE33_01055"
     CDS             4393..>5309
                     /locus_tag="VHE33_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HVT96062.1"
                     /translation="MASAAISHPKRRPPPFRGMGRATLLTLPMALWALLMLTPTLRAP
                     GIKQPIAAAAANLFMTTLFFLMMRTGKTYCWRRYFFVALGLLFPVGFIASLIHLRGSM
                     SLSLESMLGGDTPFCHMVIPMILIPAALTRTIIFPGSILPTAANPHSIAFMIGLWMAF
                     TLVMGKAWCSYGCFFGGIEEGFAALPKRIRIRKIDPRLRLVPWALLISVAVLSAALFE
                     PVYCRWLCPFKTVTEFQAVTSVQTAIQMAIFVSLFVGLVVVFPFLTKKRTQCGFFCPF
                     GAFQSLFNKINLFEIRFDRTKCVDCTLCQT"
BASE COUNT          917 a         1746 c         1476 g         1170 t
ORIGIN      
        1 atgtccatga cttcgcgaat ctcgggtgtg gcgagcgggg ctttcacgga atcctccaga
       61 gcagtgaggc acgctctttg taccacagtc cggaggcgga aaggaatcac gatttggcgg
      121 ggtattggac tatgaacccc acccccttca aatatccaac catgaaggag atggggctca
      181 acccttaaag cttcggctcc agcgcaccga tcagggacag gaagctgtcg tcgagcgtgc
      241 ggtttttcga aatcgcttct gccagcggga tgtcctcgat ctttgttccg tgcagcacca
      301 ccagccgccc aaacttgccc tgatggacga gatcgatcgc ggcgagcccg taccgcgtcg
      361 ccagcatgcg gtcgtacgcc gttggggtcc caccgcgctg cgtgtgtccc aggttgacgg
      421 agcgcgtctc gaacccagtc tttttctcga tctcttgtgc caggtgctcc ccgatgccgc
      481 ttagctgcgc gtgcccgaac gagtcaacct tcgttccttt ggtcgcctgg ccgccgatct
      541 cgggcagctt cgccccctcg gcgaccacaa cgatcccgta cttcttgtcg cctgcgtagt
      601 tcttcttcag aatcgcgcat acgtgatcga ggtcaatcgg cttctccggc accaggatca
      661 catgcgcgtt ccctgcgatg cctgccgtca gcgcgatcca tcccgcatcg cgtcccatga
      721 cctcgcacac gatcacccgc tcatgcgacc tggccgtggt gtgcagccga tccaccgcct
      781 cggtcgcgat catgaccgcg gtgtcgaacc caaagcagac atcggtgcca ttcaggtcgt
      841 tgtcgatggt cttgggcacg cctacacccg gcacaccggc ctggatcagc gcattcgtca
      901 ccgactgggt gtcgtcgccg cccagcgcga tcagcgcgtc gatcttattg gccttgagca
      961 cctgcacgca cttctcgatg ccgccctcga cttttcgaac attcgtgcgc gaggtgcgca
     1021 ggatggtccc gccctcggtc aggatgccgt cgaccttttc cagcgtcagg gaaactgctt
     1081 tgttctccag cacgccgcgc cagccttcaa gaaacccgac gaactcatcg ccgtagtggt
     1141 taatgccctt cctgaccacc gcatggatca cggcattcag acccgggcag tctccgccgc
     1201 ccgtcaacat accaattcgc atcgctgtct cccctggatt aaaactgaaa accctgtcgt
     1261 ggaagaatca cgtcactctt cgggagaagc ccgcgccact caggcggggc ctctcaagct
     1321 cgatgaaaaa cgcaggacct ttacggtcat gataccggcg gctcgtcctg ctattcaaat
     1381 gacgcacgta atgtagacca tcgttagaat cactccatgt ctctcctcaa ggtgatagtg
     1441 ctggccgtga tccaggggct tgccgagctt ctccccgttt cgagctccgc ccacgtcgtc
     1501 gttgccgaga agctcatggg catggaccca tccgcgcccg cgatgaccct gctgctggtc
     1561 atgctccaca ccggcaccat gttcgccgtc atcgtctact tctggaagca gtggaagcag
     1621 accttctttt ccgacggcga ggccttcaag cgctttgcct ggcaagcgat catcgccacc
     1681 ttctgcacgg gtgttgtcgg cgaagtagtg atcaagatca tcgagcacac ccttttccgc
     1741 ggcgcgccca aagcccagat tgaagacctc ttcagccatc ttgagatcgt cgcccctgcc
     1801 ctgctggcag ccggaattct gatcctggtc gccggcctca aggagggccg ccggcccgat
     1861 gcccaggaag ctcccgagat cggcttcggt caggccggca tcatcggcgc cgttcaggga
     1921 ctttgcctgc ccttccgcgg attctcccgt tccggcgcca ccatctccgt cggcattctg
     1981 cagggcatta agaaggtgcg cgtcgaagcc ttcagcttcg ccttggccgt catcatcact
     2041 ccgctcgcca tcggacgcga agtcctgcgt ctgcgtcacg cggactcaac cggcttcgcc
     2101 catgccgcag cgcccagcct cctcgggatg gtcttttcct ttgcggctgg actgctcgcc
     2161 ctcaagtggc tcagcgcatg gctggagcgc ggccgctggt atctgttcgg catctactgc
     2221 atcgtcgcct ccatcgtggt gttcgctctg cacgctaaag gctattgaag agaaccgccc
     2281 gcccgttcct tctggatccg ccgtgttttt ctgaacgccg aacggctgta cgctcgcttc
     2341 tatgtggctc cgcaagctcc gcatcgatcg cattgcaccg aacggcgagg cgattccctg
     2401 cccgatcgcc tggatcgaca acttcgccat gcgcaacttc accaatgatg cgatcttcga
     2461 cgacactctg ccggccggtg acggagttct cgaagccgga cagcgcgtac cgctcgatcg
     2521 cctgcaggca gcgatggacg actggttccg gcgcaaaggt tacatcaagc ccgacgaaat
     2581 gctgcgcgtc gaagaggcgc cccggtagct gccgtcattc cacgggttcg cccagcgcgt
     2641 agcgcagata gatgtactgt tcgaagggcg ctttctgcgc ggcaaaggtc agatccttcg
     2701 catactcgac gccatcgtga atcagatcgt cgtcgtcggc atcgatcagg tggaccttgt
     2761 gaacgaggcc atcggcattg acctcgacct tgaccaggag attgccgtaa agcgggtcga
     2821 gctgtaatgg tttcacgccg atcactttgt gcgggacggc gttattgtct tccggctctc
     2881 ccatgtccgg ctcatttgcc ggaatcgtga acagatcggc cggaaacgcc ttcactactt
     2941 ccgcgctgcc ggaagcctcc accataattc ggcccgcgtc atagatgcgg aaccgccgcg
     3001 gcaccatgtg ggtgccaaca ggctgccagt cctcccacgt cactgtctgg ttgaacatct
     3061 cggcaaaagc cagcacgtgc gcaaacggat cgaagcagac acgcatctgc atctgctcgt
     3121 tcgtgcaggt gaacggagaa ccctcgacct gctgaaagtg aacgtagtgg ttgggctggg
     3181 gaatggcaaa gaacgacacc gcgaaatcgg gctgccgata gttttcaagc aaatcgtgca
     3241 ccttaagcgg catcagcccg tcgaagtgcc gccattgccg ggtatgcgtt aggtcttcga
     3301 tctgggtgta atgaaaatcc ccggcgacaa tgtcggtgcg cgtaaagcgg agcggatccc
     3361 gccagatgtc ccacgtcccc ttcgtgcgtt tgccggtatg actgtaaagc ttcaggtcgt
     3421 agtggacgtg gtgcggaaca tcgctggcca gcgcgccggc ctccagcgat cgagcgtgag
     3481 cgatcgcttc agaaaatggc gggtacgaga cgacctgcgc gagggccgcg ggccattccc
     3541 agagcacgat caacagcaac aacaccatcc gcatcgccag cccctcacct cagcaaaacg
     3601 cgtcatcata acagacggcc ggggtcgaaa aaggccccat cgcagcgtcc tcccgaatcc
     3661 tcgtgatggt ggtcaccgcc aggtctctcc tccgcggatt agcatcgccc gcaatggcgg
     3721 ccccgttcct ttacaggtct atggtggagg ctctgatgct gggcctcgcg acaggtccgg
     3781 tctgcctcgc ctcctgcggg ccggtcgtat tgccatggat gctcgtacaa ccgcagggca
     3841 tccgcgtgca cgcccgtcag atgactttct ttctcggcgc gcgcctcgcg gggtatctgc
     3901 tctttgcatc cctgatctgg ctggcaggat ctgcaatcgc tcagtcgtgg ggagagcaca
     3961 cctggatgta tggggctgtg cagattttgc tggcgggtgg actgctgttc tacgcggcgg
     4021 gctggcctcg cgcgcactgt gctgtagccc aatcggaacc gccggggctg gtgcagattg
     4081 cgacttcacc tgtggagcct ccgcaatcgc ggccgacggg cgccatggcg ctcggattcc
     4141 tcaccggaat caacctctgt ccgcccttcc tcatcgccgg tgtgcgagcc gcgacgctgc
     4201 cgcagatcca cgacgctctc ttcttctttg cggtcttttt cgtggggact gccgtgtggt
     4261 tcatcccctt cctgtcgctg agcgtagttc ggcgcacccc atctttcctc accgtggcac
     4321 gcatggtggc cgttctgctg gcctgctggt acggcttcag cggagcctcg ctcctgatcc
     4381 agaaggtcat ctatggctag cgcagctatt tcgcatccga agcgacgccc tcctcccttt
     4441 cgaggcatgg gtcgtgcgac tctgcttact ttgcccatgg ccctttgggc cttgctgatg
     4501 ctcactccca ccctgcgcgc gcccggaatc aagcaaccca tcgccgctgc cgccgccaac
     4561 ctcttcatga ccacgctgtt cttcctgatg atgcgcaccg gaaagacgta ctgctggcgg
     4621 cgctatttct ttgtcgccct tggccttctc tttcctgtgg gattcatcgc cagtctcatt
     4681 cacctgcgcg gcagcatgag tctctcgctc gagagcatgc tgggcggcga cacgcccttc
     4741 tgtcacatgg tcatccccat gatcctgatt ccggcagccc tgacgcgtac catcatcttc
     4801 ccgggatcga tcctgcccac tgcggccaat ccgcactcca ttgccttcat gatcgggctc
     4861 tggatggcct ttactttggt catggggaag gcatggtgca gctacggctg cttctttggc
     4921 ggcattgaag aagggtttgc cgcactgccg aagcgcatcc ggatcaggaa aatcgatcct
     4981 cgcctgcgcc tggtgccctg ggcgcttctc atttccgtcg cggtgctgtc ggcggctttg
     5041 tttgagcccg tctattgccg gtggctctgt cccttcaaaa ccgtgaccga attccaggcc
     5101 gtcacctcgg tgcagactgc catccagatg gcgatcttcg tcagcttgtt tgtgggcctc
     5161 gtcgttgtct tcccttttct gacaaagaaa cgaacacagt gcggcttctt ctgccccttc
     5221 ggcgcgtttc aatccctgtt taacaagatc aacctcttcg agattcgttt cgaccgcacg
     5281 aaatgcgtcg actgcacgct ctgccagac
//
LOCUS       DASZYN010000036         2375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1029641, whole genome shotgun sequence.
ACCESSION   DASZYN010000036 DASZYN010000000
VERSION     DASZYN010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029641"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /gene="tilS"
                     /locus_tag="VHE33_01060"
     CDS             complement(<1..812)
                     /gene="tilS"
                     /locus_tag="VHE33_01060"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HVT96063.1"
                     /translation="MTRPPSQGRISFDCTYLRPGMRVAVAVSGGADSVGLLRTLHDAA
                     SQIGLVLSVAHVHHGIRGPEADQDQHFVAELAASLGLEVSPHRVDTPAIAREKKQTLE
                     EAARDLRYTWFRELLAQGKVDAVATAHTLDDQAETVLHRLLRGAWTEGLGGIHPIVQC
                     PPGVILRPFLETQRDEIRAWLRSIGQTWREDATNDDTAHTRNRIRLELLPHLAEYNPQ
                     IAEQLAHLATLARDEHEWWHTELARILPGLILPGRPVRGGGRAVSTHPEEGG"
     gene            843..1313
                     /locus_tag="VHE33_01065"
     CDS             843..1313
                     /locus_tag="VHE33_01065"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT96064.1"
                     /translation="MLTTRPATAADAPLITHHRRRMFVDAGRRDNQVLDLMAQSHEPW
                     VAQAITDGKYLGWLSEDDGKVVAGAGLLLLDWPPHPLDPHSSKRGYLLNVYVEPEYRR
                     RHLATNLIELALAEARRRGIRVVALHSTEEGRRLYEAAGFRTTNEMFYVESIEP"
     gene            complement(1366..1956)
                     /gene="sufT"
                     /locus_tag="VHE33_01070"
     CDS             complement(1366..1956)
                     /gene="sufT"
                     /locus_tag="VHE33_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HVT96065.1"
                     /translation="MIITNQTVPLKRDCEAIAVPAGTKQMLPAGTCVKITQAAGSSYT
                     VSASGALYRIDEKDADALGQSGPATQPHTPPAFSERMVWDQLKTIYDPEIPVNIVDLG
                     LIYACEIADHPEGGKKIDVQMSLTAPGCGMSDVLKADVEKKLAHLPEVKSVKVEVVFD
                     PPWNPGRMSEAAKLQLGFDLDYGTNSNSFGPFPILR"
     gene            complement(1986..>2375)
                     /locus_tag="VHE33_01075"
     CDS             complement(1986..>2375)
                     /locus_tag="VHE33_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HVT96066.1"
                     /translation="RNYKALESANHKAEGYNPLCGDRFTVYLQMDGDTIREIGFQGAG
                     CAISKASASMMTQSVKGKTKEEAEKIFHRFHDLVTGHSHADQAELGKLAVFSGVSEFP
                     VRVKCATLAWHTLEAALEGKQEKISTE"
BASE COUNT          406 a          694 c          779 g          496 t
ORIGIN      
        1 atgccgcctt cttccggatg agtcgaaacg gcgcgacctc cgccgcgcac cggtcttccc
       61 ggcaggatca ggcctggcag gatgcgcgcc aactccgtgt gccaccactc atgctcatcg
      121 cgcgcaaggg ttgcgaggtg cgcgagttgc tcggcgatct gcggattgta ttccgcgagg
      181 tgaggcagca gttcgaggcg gattcgattg cgggtgtggg cggtgtcgtc attcgttgcg
      241 tcttcgcgcc aggtttggcc tatggagcgg agccacgcgc gaatttcgtc gcgctgggtt
      301 tcgagaaagg ggcgcagaat gacgccaggc gggcattgca cgatggggtg tattccaccg
      361 aggccttcgg tccatgcgcc gcggagcagg cgatggagca cggtctctgc ctgatcgtcg
      421 agggtgtggg ctgtggcgac ggcatcgact ttgccctggg ctaacaattc acgaaaccac
      481 gtgtagcgaa ggtcccgggc cgcctcctcc agagtctgtt tcttttctcg cgcgatggcg
      541 ggggtatcga cgcggtgcgg cgacacctcg aggccgagtg aagctgccag ttctgcgacg
      601 aagtgctggt cctggtcggc ttcagggcca cgaatcccgt ggtggacatg ggcgaccgat
      661 agcaccaggc cgatctgcga cgcagcgtca tgaagcgtcc gcaggaggcc gaccgaatcc
      721 gcaccgccgg aaactgcgac ggccacacgc atgccggggc gcaggtaggt gcagtcgaaa
      781 ctgattcggc cctgactcgg gggtcgtgtc acgtcactat cgtaagctct atacttctca
      841 tcatgctcac gacgaggcca gccacggccg ccgacgctcc gctcatcacc caccaccgac
      901 gccgcatgtt tgtggatgcg ggccgacgcg acaaccaggt gctcgatcta atggcccagt
      961 cgcatgagcc gtgggtggcg caggccatca cggatggcaa ataccttggc tggctcagcg
     1021 aagacgatgg aaaagtggtc gcgggggccg ggcttctgct tcttgactgg ccgcctcatc
     1081 cgctggaccc ccactccagc aagcgcggct acctgctcaa cgtttatgtc gaaccggaat
     1141 accgccgtcg ccacctcgcg acgaacctca tcgagcttgc tcttgctgag gcgcgccgcc
     1201 gagggatccg cgtggtcgct ctgcactcga ccgaggaagg ccggcgtctg tacgaggctg
     1261 caggcttccg cacgacgaat gagatgttct acgtcgagtc gattgagccg tagaagcaga
     1321 gtttagagtt tagagttttt tgcgagacga gccgcgccgc aatcactagc gcagaatcgg
     1381 gaacggtccg aaagaattgc tgttggttcc gtagtcgagg tcgaagccca gttgcagctt
     1441 cgcggcctcg gacatgcggc cgggattcca cggcggatcg aagacgacct cgaccttcac
     1501 ggacttcact tcgggcaggt gcgccagctt cttctcgacg tcggccttga ggacatcgga
     1561 catgccgcat cctggcgcag tcaacgacat ctgcacatcg attttcttac cgccttcggg
     1621 atggtctgcg atttcgcagg cgtagatgag gccgaggtcg acgatgttca cgggaatctc
     1681 gggatcgtag attgttttca gctggtccca caccatgcgc tcgctaaacg cggggggagt
     1741 atgcggctgg gttgcggggc cgctttggcc gagggcgtcg gcatcctttt catcgatgcg
     1801 atagagcgcg ccgctggccg aaaccgtata gctcgaaccg gcggcctggg tgattttcac
     1861 gcaggtcccg gccggaagca tttgcttcgt gccggcggga accgcgatgg cttcgcagtc
     1921 tcgcttcagg ggaacggttt ggttggtgat gatcatgaaa tggcccgtgc ctgttgccga
     1981 tggcgttact cggtggagat cttttcctgt ttgccttcga gcgcggcttc gagggtgtgc
     2041 caggcaagag tcgcgcattt cacgcgcacc ggaaattccg atacgccgga gaagaccgcc
     2101 agcttgccga gttcggcttg atcggcgtgg ctgtggccgg tgaccaggtc gtgaaagcga
     2161 tggaagatct tttccgcttc ttcctttgtc ttgcctttga cgctttgcgt catcatggag
     2221 gccgacgctt tggagatggc gcaaccggcg ccctggaagc caatctcgcg aatggtgtcg
     2281 ccgtccatct ggagatacac cgtgaagcgg tcgccgcaga gcgggttgta gccctcggct
     2341 ttgtggttcg cgctctcgag cgccttgtag tttct
//
LOCUS       DASZYN010000037         2468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_130342, whole genome shotgun sequence.
ACCESSION   DASZYN010000037 DASZYN010000000
VERSION     DASZYN010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130342"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VHE33_01080"
     CDS             <1..204
                     /locus_tag="VHE33_01080"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HVT96067.1"
                     /translation="EMEIVWRDLTPATVTTFETMTGPDLKALNTFADPKKVVPQTLDK
                     PKTGSRMTVKLPARSYSVLSMTV"
     gene            complement(225..428)
                     /locus_tag="VHE33_01085"
     CDS             complement(225..428)
                     /locus_tag="VHE33_01085"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96068.1"
                     /translation="MTQAAVANEAHVSRSTLDALENSRLGELGYSKIANILAVIGLEL
                     TLNEATGRRPTLDELMVENQKEE"
     gene            complement(623..1786)
                     /gene="dnaN"
                     /locus_tag="VHE33_01090"
     CDS             complement(623..1786)
                     /gene="dnaN"
                     /locus_tag="VHE33_01090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HVT96069.1"
                     /translation="MPAVEEEKAVQGATMEISVSRQDLLRELTATQSVVERKTTIPIL
                     SNFLLEADGDRIVITATDLDQSIRTSCAAKVKKPGSCTIPARKLYDYIRLLGDGDMSI
                     KLLENHWVQIRAGRSNTRMVGMARANFPQVPEFPSGSAIKIPVASLRTLIARTIFAIS
                     NEESRYTLNGALLILKAESIAMVATDGHRLAHIEKAGENFPGINAEKKTLVPRKALQE
                     LQSLLANTEAEHLEFADDDSTLFFRVGHRVLTSRKLTGQFPNYEAVMPRDNNKFAIVR
                     SPDLSASIQRVAQFADERSGAIKMRLEQNEMKVSSSSTDAGESEDTIETPYSFDPIVV
                     GFNSAYLIDFLKAIGNEGEVRLEFKDAQSAGQIRPEESSDEYKYRYIIMPMRI"
BASE COUNT          475 a          618 c          859 g          516 t
ORIGIN      
        1 gagatggaga tcgtgtggcg ggatctgact cctgcgacgg tgacgacgtt tgagacgatg
       61 accgggccgg atctgaaggc gctgaacacg ttcgccgatc cgaagaaggt cgtgccgcag
      121 acgctggaca agccgaagac gggatcgagg atgacggtga agctgccggc gcggtcgtac
      181 tcggtgttgt cgatgacggt gtgagcggaa gcgccgggtc gtccttattc ttctttctgg
      241 ttttctacca tcagctcatc gagcgtgggc cttcgacctg tcgcctcgtt caaggtcagc
      301 tcgaggccga taacggccag gatattagcg attttcgaat agccaagctc ccccagccgg
      361 ctgttctcca gcgcgtccag cgttgagcgg ctgacgtgcg cctcgtttgc aacggcggct
      421 tgagtcaaac caggtctttt ctcttagcag cgatccgctc gccgagatcg ggaaggagga
      481 gcagcacgtg cctaatatac cgggcagttt tggaggatgg cacagttgtt gctttgaata
      541 ttgcgcagcc acgcgccaag tgggtcctgg aggcggacga cgcccaaaga ggaggatgat
      601 aattaacgtt acgctcgccg cttcagatcc gcatgggcat gatgatatag cggtatttgt
      661 attcgtcgga gctttcttcg gggcggatct ggccggcgga ctgggcgtcc ttgaactcca
      721 ggcgaacctc gccctcattg ccgatggcct tgaggaagtc gatcaggtac gcggaattga
      781 agccgacgac gatgggatcg aagctgtagg gagtctcgat ggtgtcttcg gactcgcccg
      841 cgtcggttga agacgacgaa accttcattt cgttttgctc gaggcgcatc ttgatggcgc
      901 ccgagcgctc gtcggcgaac tgcgcgacgc gctgaatgga ggcggagagg tccggagagc
      961 gcacgatcgc gaacttgttg ttatcgcgcg gcatcacggc ttcgtagttg gggaactggc
     1021 cggtgagttt gcggctggtg agcacgcggt ggccaacacg gaagaacagc gttgagtcgt
     1081 catcggcgaa ttcgagatgc tcggcctcgg tgttggcgag gagcgattgc agctcctgca
     1141 gcgctttgcg cgggacgaga gtcttcttct cggcattgat gccggggaag ttttcgccgg
     1201 ccttctcgat atgagccagg cgatggccgt cggtggcgac catggcgatg gattcagcct
     1261 tgaggatcag cagcgcgccg ttgagcgtgt agcgcgattc ttcgttggag atggcgaaga
     1321 tggtgcgtgc gatgagggtg cgcaaagagg cgacgggaat cttgatggca ctgccgctgg
     1381 gaaactcggg cacctgggga aagttggcac gcgccatacc caccatgcgg gtgttggagc
     1441 ggccggcgcg aatctgcacc cagtggttct ccagcagctt gatcgacatg tcgccgtcgc
     1501 cgaggaggcg gatgtagtcg tagagcttgc gcgcgggaat ggtgcaggat ccgggcttct
     1561 tcaccttcgc cgcgcagctg gtgcggatgc tttgatcgag atccgtggcg gtgatgacga
     1621 tccggtcgcc gtcggcttca agcaggaagt tggagaggat ggggatcgtg gtcttgcgct
     1681 cgacaacgct ttgcgtggca gtcagctcac gcagaagatc ctgacggctg acgctgatct
     1741 ccatagtcgc tccctgaacc gctttctcct cttcgacagc cggcatgatg gctttccccc
     1801 gaatcgattg gactcactgt acaccaggcg gggcggcaat ccgcaagcgc acaggaccga
     1861 agaatccatt ctagagtcac ttgtcggaat tgcagcctct ggaaaacggg gagtttttgt
     1921 gggttgtgcg gagacgggcg aaccaagcct tcggcgacgg ctacgaatag gtagatgaat
     1981 ctcttctttc tccagtagtc gtagtcgttc gcactggaaa agtggaaagg cgagaggaaa
     2041 ccgctggtga agcggagcgc gggaggtgcg cgttttagaa aaagcggctg agggaaagag
     2101 gagaatgcgg aaacctggag gagaacgccc cttgcgggca ctgaatgtcc acgggtttca
     2161 caggcgcagg gaaaacgggt gtggaaaggt ggcggccgca agtggaaagg cgggtccggc
     2221 gcaaaagaga agcgggttct gatggaaccc gccttccaca ttgatcaaga tgtcctgggt
     2281 ttgccggggg attgggaggg ggagggccgg gggaggaaaa gctgagcaaa actccggccc
     2341 gctgaggatc agtcggtgct catggtgtcg ttgtggctga cggtgatgcc gccgttgacg
     2401 gtctgcacgt gcacggtcgg tccgccactg ccgaggtcgg tgttgaggtg gttcttgatg
     2461 gttccttg
//
LOCUS       DASZYN010000038         9920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_837222, whole genome shotgun sequence.
ACCESSION   DASZYN010000038 DASZYN010000000
VERSION     DASZYN010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 9920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9920
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837222"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VHE33_01095"
     CDS             <1..261
                     /locus_tag="VHE33_01095"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HVT96070.1"
                     /translation="APFLFRQIDAIRPEVIVALGATAATYLLGGKSPLAALRGRIHHA
                     RDSKLIVTYHPAYLLRDPRQKKEAWADLQLAMTELGLKPPAK"
     gene            complement(335..1072)
                     /locus_tag="VHE33_01100"
     CDS             complement(335..1072)
                     /locus_tag="VHE33_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT96071.1"
                     /translation="MAAEKVAFITGGNKGLGFETARQLGQLGVKVVIGSRDLARGKQA
                     VEKLKADGVDAEVVRFDITKPADYKEIYTLLDRKYGRLDILVNNAGVSREDFMGGNKT
                     SSTSAEVLHETFDTNFFGAVQLTQTLLPLIKKSPAGRIVNLSSILGSLALHADPQSPI
                     YNAKAFAYDASKAALNSFTVHLAHELKDTKIKVNSAHPGWVKTDMGTDAAPMEIADGA
                     KTSVALATLPDDGPTGAYIHLGETLPW"
     gene            1141..3639
                     /gene="priA"
                     /locus_tag="VHE33_01105"
     CDS             1141..3639
                     /gene="priA"
                     /locus_tag="VHE33_01105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HVT96072.1"
                     /translation="MDVSLLLRSEKDPMPAFCDVALPVPLDRPFTYGVGDLQLCVGAR
                     VLVPFRNEKLAGVVIRLHDEPPPVDARPLLAVLDDEAVITPELMELAIWIAQYYVAPI
                     GEVLRSMLPLGGEVRRQVLYRITDIGRQVLLAAAEQGSSRRSRLSSEEQDQEYAVLNE
                     LASGEAVRATRLRSSTGISARILQGMLRKKWIARETAAEARDARRTVRYAVLIEDVRL
                     PKLNENQQAILAELAGADGELPVAELRRLTVPASTLTTLVRRSLVRIEERPADFHLTH
                     LHAFLTPEHDLNPAQLEALDTITAAVAAHEFRPLLLHGVTGSGKTAVYLAAMRRALAA
                     GRTTILLVPEIGLTPAMAAQLHHAFGSEVALLHSALTPEERGEQWRRIRKGEARVVVG
                     TRSAIFAPVENLGLVIVDEEHDSSYKQESTPRYNGRDVAVMRARSANATIVLGSATPS
                     LETWHNSTRGKYQCIELRERVKDRPLPEVRLIDMRREFQETGEEKLFSRALIDETQAT
                     LDRGEQAIILLNRRGYSFVVICRACGAKLECENCSIALTYHKPLPGDGHAAGQRLECH
                     YCGYKKTVPKTCPACGSEYLYFLGAGSQQGEERLQEIFPNARIGRMDRDTVRGRSDME
                     RLLLRLHSGEINLLVGTQMIAKGHDIHGVTLVGVVGCDYALGLPDFRASERVFQLLTQ
                     VSGRAGRGDLPGQVIVQTYHTDHYAILFAAEHDYAGFVEKELKFRRMMRYPPYAALAN
                     VLLQSQRLEEAAAWANILGRWCQHTTLRNVRVLGPATAPIAKIKRTYRFHLVLKGETR
                     PDLQKALRALLAHAEEAQIPRAHLIIDVDPVSLM"
     gene            3656..4585
                     /locus_tag="VHE33_01110"
     CDS             3656..4585
                     /locus_tag="VHE33_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HVT96073.1"
                     /translation="MQFLDEDKVRSLLTYQDLIPAVEQALINLSLRKIQQPVRTILRV
                     PEQDSDRTRGFFALMPAVDGDLMAVKLVTFYEGNTSVPTHQAVIQLLSAQTGEPLLAM
                     DGRLITEMRTAAVSALATRLFAPKDARTLAILGSGVQARSHLEALRLVRNFSAVRVWS
                     RTPANAERFAAKWDGTPVPTPEAAVRGADVVVAVSSVHEPLLRGAWLGPDTFVCAVAV
                     VTPDRRELDDAAMEAAIVVESREAALRESGDIIGSGQPVYAELGEILGGAKAVPKGRI
                     VFKSLGVAACDLAAAEVVWRCLKSAQTLKTKVT"
     gene            4673..7078
                     /locus_tag="VHE33_01115"
     CDS             4673..7078
                     /locus_tag="VHE33_01115"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVT96074.1"
                     /translation="MRKFSLFLVVCGIAWGIAFSARTIAAQSSIVSSSAAGPPHLLTT
                     VAQVRELSNTEAAKSIPVELHAIVTYYQPTEGQVFVQDATGGIYIASPPNPAVLNPGD
                     AVLVRGTTVPSYSVNVKATEMKFEHRADFPEPVALSWREFLKKSNDCRYVTISGKVRS
                     ATLQASIGQFSQGGVQQRGMSPAQIAESIAKLGGRQPYLLIDLQTEGGLVRVHMENVL
                     GIDPFRLLDAQVSFTGVAGGLFDGKFQLTGAELWVSTAQHMRILSVPPSDPTQLPLTS
                     INHVMLGSFVRDESQRIHLRGSVTLYQPGLEMVLQTAEGDAVEVNSFEQTPLSIGQVV
                     DVVGFPDPHQYSEALTDANVLPTEATQPIEPKAVNWTDTIAGRYPFNLISMEGKLAAE
                     VHEPHQDTLVIQAGPHVFSAILPRTVWNQDLKEAVLPDYPIGSTVRIAGVCFVHAGGP
                     WNTERWFDLQLRTPEDVTVLVAPSWWTVRRLLYVSAALLFLMLTALVWAFMLQNKVRR
                     QSEQIRVTMESEAARERRIAFLEKERGRVLEAINSMLNMDDVLLMILRLISTQLEDRS
                     CWCELANGTIVGQAVASQDSIDVVRRGIYSGAGERLGSVVVSGAEACQEQAGEALEMG
                     ASLAALAIDNRRLYETLIHRSQYDQLTNAANRFLLESRLDEALNHAKRSHTHFALVYI
                     DLDQFKQVNDFYGHRAGDVYLQQVAERLSEKLRGMDTLARVGGDEFIALIPVVRNRGE
                     VGEIIDRLSHCFDAPFAVDDYTISGSASIGYALYPEDGATKDELKRVADAAMYANKPH
                     AAV"
     gene            complement(7135..8601)
                     /locus_tag="VHE33_01120"
     CDS             complement(7135..8601)
                     /locus_tag="VHE33_01120"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HVT96075.1"
                     /translation="MNAISYAELLRATSPEIVVAVTGLVLLALDLSVMRRGAARRRFG
                     AGALIACIGSLVAIWMLMHGTGTMSLPDAMFVVTPLTRVVQMALLALGILTVLLSTST
                     RITEHVGEFLALIVFATVAMMFLVATQNLLLIFVALEFLSLSLYILTGFDKRSRQSAE
                     AGLKYFLFGGMSAGFLLFGMSLLYGVSGSIELPQIAAAISGPSLDPLVLIAIVMVAIG
                     FGFKVAAAPFHLWAPDAYQGAPTVSAGFIASSSKVASFFIFAQVVTIGLATAIGSGAY
                     RAYTPGWVPVLCAIAALSMIVGNLAAIAQTSVRRLLAYSAIGHAGYMLLGLIAHSAMG
                     ERALIYYVITYALASLGAFGVLGTLEAEGIDKISDLAGLHRRAPGLSLCLLVFVLSLA
                     GIPPLSGFFGKFYIFAAAADAEPKLGLLWLVLLAVAMSAMSLYYYLKVLKQVYVAEGS
                     VQAEPLRISLVSRATIWALAALVVLLGCAPGLLLRWLP"
     gene            complement(8598..>9920)
                     /locus_tag="VHE33_01125"
     CDS             complement(8598..>9920)
                     /locus_tag="VHE33_01125"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HVT96076.1"
                     /translation="AADGISLVLVLLTGLAATAGILFSWNIEKRTQEFFAFYLVLIGG
                     VYGVFLSFDLFLLFVFYEIAIIPKYFLIAIWGSSKAGATGEEAGSQREYAAMKLALYS
                     FGGSALVLAGMVATWVATGGHSMSFDTLTAAHLSTSLQMALFPLVFVGFAVLAGMWPF
                     HTWAPTGHAAAPTAASMLLAGVVMKLGAYGCLRVAMMLFPAGMGPWGFHVLGLGSWRE
                     VFALLAVIGIVYGASVALVQRDFKFVIGYSSVSHMGFVLLGLMTLTQIGLDGAVLQMF
                     SHGVIAGLLFAVVGRMVYDRTHTRDLNELGPMQLGRAIPFAAATFILAGMASIGLPGF
                     SGFIAEFQVLVGTWRSFSWMVVIAGIGILVGIAFTWRALTKAFYGDPTPGAAEHVLPP
                     ISSPEKLAALLLLASTLAVGLYPRVLTDIILPALNSPLFEGLRQGAWR"
BASE COUNT         1975 a         3272 c         2878 g         1795 t
ORIGIN      
        1 gcgccgttcc tgttccgtca gatcgacgca atccgccccg aagtgatcgt cgcgctcggt
       61 gcaaccgcag cgacgtacct gctcggtggc aagagccctc tcgccgcatt acgcgggcgc
      121 atccaccacg cgcgcgactc gaagctgatc gtgacctacc atcccgccta tctgcttcgc
      181 gatccgcgtc agaaaaaaga ggcttgggca gacttgcagc ttgctatgac ggaactgggg
      241 ctgaagccgc ctgcgaaata gccgacagcg taatcgaaga agcctcgctt gataccggct
      301 ccggattacg ctgccgacct cggtcagctg cgcttcacca cggcagcgtt tctcccagat
      361 gaatataggc gcctgtaggg ccatcgtcgg gcagcgtcgc gagcgccacg ctggtctttg
      421 cgccgtccgc aatctccatc ggcgctgcgt ccgtccccat gtcggtcttc acccagccgg
      481 ggtgtgcaga attgaccttg atcttggtgt ccttcagctc atgcgccagg tgcaccgtga
      541 acgaattcag cgcagccttc gatgcgtcgt aggcgaacgc tttggcattg tagattggcg
      601 attgcggatc agcatgcaac gcgagcgatc cgagaatact cgacagattc acgatgcgcc
      661 cggccgggga ttttttgatc agcggcagca gtgtctgagt gagttgtact gcgccgaaga
      721 agttcgtgtc gaaagtttcg tgcaggactt ccgcgctcgt gctgctggtt ttgttgccgc
      781 ccatgaagtc ttccctggaa acgccggcgt tattgaccag gatgtcgagc cttccgtact
      841 ttcggtcgag cagggtgtag atctctttgt aatcggcagg tttggtgatg tcaaagcgaa
      901 ccacttccgc gtcgacgccg tctgccttca gcttttccac agcttgcttc ccccgcgcca
      961 gatcgcgcga tccgatgaca accttgaccc ccagctggcc taactgacgg gcagtttcga
     1021 aaccaagccc cttgttgccg ccggtgataa atgcaacctt ctctgcagcc atccaattcc
     1081 tcctcgtcac catgtgattc acgagaggcg gcaaggattc acgcagcctg cgaaacccac
     1141 atggatgttt cgcttctgtt acgctccgaa aaagacccga tgcctgcctt ctgcgatgtc
     1201 gccctgcccg ttccacttga tcggccgttt acctacggcg tgggcgacct gcagctctgc
     1261 gttggggcgc gcgtgctcgt tcctttccgc aacgaaaagc tcgccggagt ggtgattcgc
     1321 ctgcacgatg aacctccccc cgtggatgcc aggcctctcc ttgctgtgct cgacgacgag
     1381 gcggtgatca cgccggagct gatggaacta gccatttgga tcgctcagta ttacgtggct
     1441 cccattggcg aagtcctccg ctccatgctg cccctgggcg gcgaagtgcg gcgccaggtg
     1501 ctctatcgca tcaccgatat cggccgccag gtgctcctcg ccgcggccga gcagggctcc
     1561 tcacgtcgtt cccggctttc gtctgaagaa caggatcagg agtatgcagt cctcaacgaa
     1621 ctggcgtccg gtgaagccgt tcgtgcgacc cgccttcgct catcaacagg aatttctgcg
     1681 cgcattctgc agggcatgct gcgcaagaag tggatcgcgc gtgagaccgc cgccgaggcg
     1741 cgggatgccc gacgcaccgt gcgctacgcc gtcctcatcg aggacgtccg cctgccaaaa
     1801 ctaaacgaga atcagcaggc gattctcgcc gagctggccg gcgccgatgg cgagttgccc
     1861 gtcgccgaac tgcgccgcct gaccgttccc gcctcgacgc tcaccacact cgttcgcaga
     1921 agcctggtcc ggatcgaaga gcgccccgcg gacttccatc tgacccatct ccacgcgttc
     1981 ctcacgcccg agcatgatct aaaccctgct cagctcgagg cactcgacac catcaccgcc
     2041 gctgtcgctg cgcatgagtt tcggccgctc ctgctgcacg gggtcaccgg ctccgggaag
     2101 accgctgtgt acctggccgc gatgcggaga gcgctcgccg cgggccgaac cacaatcctt
     2161 ctggtcccgg aaataggact cacccccgcg atggccgcgc agctccacca tgcattcggg
     2221 tccgaagtag cgctcctgca ctctgcactg acgccggagg agcgcggcga gcaatggcgc
     2281 cgcattcgca aaggagaagc ccgcgtcgtc gtgggtacgc gttctgccat cttcgcgccg
     2341 gtcgagaacc ttggtctcgt catcgtcgat gaagagcacg attcgagcta caagcaggaa
     2401 tccacaccgc gctacaacgg ccgcgatgta gccgtgatga gggccaggag tgccaacgcc
     2461 acgattgtgc tgggctctgc aacgccctcc ctcgaaacct ggcacaactc taccaggggt
     2521 aagtaccagt gcatcgaact ccgcgagcga gtaaaggatc gcccactgcc ggaggttcgc
     2581 ctgattgata tgcgtcggga attccaggag acgggcgagg agaagctgtt ttcgcgcgcc
     2641 ctcatcgacg aaacccaggc gacgctcgac cgcggcgaac aggccatcat cctgctcaac
     2701 cgccgcggtt attcctttgt ggtcatctgc cgcgcctgcg gcgccaaact cgaatgcgag
     2761 aactgctcca tcgccctcac ctatcacaag ccccttcccg gcgatggcca cgctgcggga
     2821 caacgtctgg aatgtcacta ctgcggttac aaaaagacgg tcccgaaaac ctgccccgcc
     2881 tgcggcagcg aatacctcta ttttctcggt gccggatcgc agcagggaga ggagcggctg
     2941 caggagatct tccccaatgc gcgcatcggg cgcatggatc gcgacaccgt ccgcgggcgc
     3001 tccgacatgg agcggctcct ccttcgcctt cactccggcg agattaacct gctcgtcggc
     3061 acgcagatga ttgcaaaagg ccacgacatc cacggagtga ctctggtggg cgttgtcggc
     3121 tgcgactacg cgctggggct gcctgacttc cgcgcatcgg agcgagtctt ccagctcctc
     3181 acccaggttt cgggacgcgc tggccgtggc gaccttcccg gccaggtgat cgtgcagacg
     3241 tatcacaccg accactatgc gattcttttc gccgccgaac acgattacgc aggctttgtc
     3301 gagaaggagc tgaaattccg gcgcatgatg cgctatccgc cgtacgccgc tctcgcgaat
     3361 gtactgctgc aaagccagcg tctcgaagaa gctgcagcct gggccaacat cctgggccgc
     3421 tggtgccagc acaccacgct tcgcaacgtt cgcgttctgg gtcccgcgac agcccccatc
     3481 gcaaagatca aacgcaccta tcgatttcac ctggtcctga agggcgaaac gcgccccgat
     3541 ctgcagaagg ctcttcgcgc gctgctcgct cacgccgaag aagcacagat cccccgcgcg
     3601 cacctcatca tcgacgtcga tcctgtcagc ctgatgtaac caggcggaga accccatgca
     3661 atttctcgac gaagacaagg tccgatcgct gcttacgtat caagacctca tcccagcggt
     3721 cgaacaggcg ctgataaatc tctcgctgcg aaaaattcag cagcccgtgc ggaccattct
     3781 gcgcgttccc gagcaggatt ccgaccgaac acgcggcttc tttgcgctga tgccggccgt
     3841 agacggcgac ctcatggccg tcaagctggt cacgttctac gaaggcaata ccagcgtgcc
     3901 gacccatcag gccgtcatcc agctgctctc cgcccaaacc ggcgagccgc tccttgctat
     3961 ggacggccgc ctgatcaccg agatgcgcac cgccgcggtc tcagcgctgg ctacgcgtct
     4021 gtttgctccg aaagatgccc gcactctcgc gattctcggc agcggcgtcc aggcgcgttc
     4081 ccatcttgaa gcacttcggc ttgtccgcaa tttctctgca gtccgcgtct ggagccgcac
     4141 ccctgcgaat gctgagcgct ttgcggcgaa atgggacggg acaccggtgc ccacgcccga
     4201 agcagccgtg cgcggggctg acgtcgtcgt cgccgtctcc agcgttcacg agcccctgtt
     4261 gcggggggcg tggctcggtc cagacacttt tgtctgcgct gtcgcggtgg ttacgccgga
     4321 tcgccgcgag ctggacgacg cggcgatgga agccgcgatt gttgtcgagt cgcgggaagc
     4381 cgcccttcga gaatccggcg acatcatcgg ttccggccag cccgtctacg ccgaactggg
     4441 tgagatactc ggcggcgcga aagcagtgcc caaaggccgt atcgtcttca aatccctcgg
     4501 cgtcgctgcc tgcgacctgg ccgcggcgga ggtggtttgg cgctgcctca aatcggcgca
     4561 aacgcttaaa accaaagtaa cttgaggggc attgcctccc ctgaaaatgc tacagacacc
     4621 tgacgctttc gtagcccatt cttaacaatg gcgccgtgag gagcgctgcc ctttgcggaa
     4681 gttttccctc ttcctggtcg tatgcggtat cgcctgggga atcgcattct cggcgcggac
     4741 gatcgcggcc cagagttcga ttgtctcgag ctccgccgcc ggaccgcctc atctcctcac
     4801 caccgtcgcg caggtccgcg agctatccaa tactgaggcg gcaaagagca tcccggtgga
     4861 gctgcacgcc atcgtcactt actaccagcc caccgagggc caggtcttcg tgcaggacgc
     4921 gaccggcggg atctacatcg catcgccgcc gaatccggcc gtgctcaatc ccggcgatgc
     4981 cgttctggtc cgcggcacga ccgtccccag ctactcggtg aacgtaaaag caaccgagat
     5041 gaaattcgag caccgcgccg attttcctga gccggttgct ctctcgtggc gcgagtttct
     5101 gaagaaatca aacgactgcc gttatgtgac gatctcaggc aaagtgcgct ccgccaccct
     5161 tcaggcttcc atcgggcaat tctcccaggg cggcgtgcag cagcggggga tgtcccccgc
     5221 gcaaattgct gagtcaatcg cgaaactcgg cggcaggcag ccgtatctgc tgatcgattt
     5281 gcagaccgag ggcggattgg tccgcgtgca catggagaat gtgctgggca tcgatccgtt
     5341 caggcttctc gatgcccagg tgagctttac cggcgtagcc gggggactgt ttgatggcaa
     5401 gtttcaactg accggcgccg agttgtgggt cagtaccgct cagcacatgc ggatcctcag
     5461 tgttccaccc agcgatccca cgcagttgcc gctcacctcc ataaatcacg tcatgctcgg
     5521 ctcttttgtg cgggacgaga gccagcgcat ccacttgcgc ggcagcgtca cactgtacca
     5581 gcccggtctg gaaatggttt tgcaaaccgc ggagggtgat gcggtcgagg tgaattcgtt
     5641 tgagcagacg cctctcagta ttggacaggt ggttgatgtc gtgggcttcc cggatccgca
     5701 tcaatattct gaagccctca ccgatgccaa cgtgcttcca acggaggcga ctcagcccat
     5761 cgagccgaag gcagtgaact ggacagatac catcgctggc cgctacccct tcaatctgat
     5821 ctcgatggaa ggcaagctgg cggccgaagt ccacgagccc catcaggaca cccttgtcat
     5881 tcaggcgggt ccccacgtct tctccgccat tctgccccgc accgtgtgga accaggacct
     5941 caaggaagcg gtcctccctg actatcccat cggcagcacc gtgcgcatcg ccggtgtttg
     6001 cttcgtccac gccggaggcc catggaatac cgagcgctgg ttcgatctgc aactgcgcac
     6061 acccgaggac gtcaccgtgc tggtggcgcc gtcatggtgg actgtccgcc gcctgcttta
     6121 cgtgagcgcg gcattgttgt tcctgatgct gaccgcattg gtgtgggctt tcatgctgca
     6181 gaacaaggtg cgtcgccaga gtgagcagat tcgcgtgacc atggaatccg aagccgcccg
     6241 tgagcgccgt atcgcgttcc tcgaaaaaga acgcggccgt gttctggaag ccatcaacag
     6301 catgctcaac atggatgatg tgctgttgat gatcctgcgg ttgatcagca cgcagctgga
     6361 agaccgctcc tgctggtgcg agcttgccaa tggcaccatt gtgggtcagg ctgtggcttc
     6421 ccaggattcg atcgacgtcg ttcggcgcgg catctactca ggcgccggcg agcgcctcgg
     6481 ttcggtcgtt gtttcgggcg cggaagcctg ccaggagcag gccggggagg cgctcgaaat
     6541 gggtgccagc ctcgctgcgc tcgccatcga caaccggcgg ctctacgaaa cgctcataca
     6601 tcgctcacag tacgatcagc tcaccaatgc cgccaaccgc ttcctgctcg aaagccgtct
     6661 cgacgaggcg ctcaaccacg ccaaacgcag ccatacgcac ttcgctctgg tttacatcga
     6721 tctggaccag ttcaagcagg tgaacgactt ctacgggcac cgcgccggcg atgtctacct
     6781 tcagcaggtg gccgagcggc tttcagaaaa gctgcgtggc atggatacgt tggcacgcgt
     6841 aggtggggac gagttcatcg ctctgatacc cgttgtccgc aaccgcggcg aggtcgggga
     6901 gatcatcgac cggctttcgc actgcttcga cgcgcccttc gcggtcgacg actacacgat
     6961 ttccgggtcg gccagcatcg gctacgccct gtatccggaa gacggtgcga ccaaggatga
     7021 gctgaagcgc gtggccgacg ccgcgatgta cgcaaacaaa ccgcatgcgg cagtttgaga
     7081 tcggtcgcag ttagtcgcgt tctgtaatca gcaacgtgtg acctgcgagc gttgttacgg
     7141 caaccaccgg agcaacaatc ccggggcgca tccaagcagc accaccagtg ccgcaagcgc
     7201 ccagatcgtc gcccgcgaca cgagcgatat ccgcagtggc tctgcctgaa ccgatccctc
     7261 tgccacgtat acctgcttga gcaccttcag gtagtagtag agcgacatgg cgctcatcgc
     7321 aaccgccaac agcaccagcc acagcagccc gagcttcggc tcggcatcgg ccgctgccgc
     7381 aaagatgtag aacttgccga agaatccgga cagcggcggg attcccgcca gcgacaaaac
     7441 gaaaaccaac aggcaaagcg acaaccctgg tgcgcgacga tgcagcccgg caaggtcgct
     7501 gattttgtcg atcccttcag cctccagtgt gccgagcacg ccgaaggcgc ccagggaggc
     7561 cagcgcgtac gtgatcacgt aatagatcag cgcccgctcg cccatcgccg agtgagcgat
     7621 cagccccagc agcatgtagc ccgcgtggcc aatcgccgaa tacgccagca accgccgcac
     7681 gctggtctgc gcgatggccg cgaggttgcc gacgatcatt gacagcgcgg cgatggcgca
     7741 gagcaccggc acccatcccg gcgtataggc gcgatacgcg ccgctgccga tcgccgtggc
     7801 cagtccgatg gtgaccacct gggcgaagat gaagaagctg gcgaccttcg aactcgatgc
     7861 gatgaaaccg gcgctcaccg tcggtgctcc ctggtacgca tccggcgccc acaggtggaa
     7921 cggcgcggct gcgaccttga atccgaatcc gatggccacc atcacgatcg caatgagcac
     7981 cagcggatcg agcgacggcc cgctgatcgc agcggcaatc tgtggcaact caatggagcc
     8041 ggaaaccccg tagagcaggc tcatgccgaa cagcaggaat cccgcggaca tgcccccgaa
     8101 caggaagtat ttcaggcccg cctcggctga ctgccggctc cgcttgtcga atccagtcag
     8161 gatgtagagc gacaggctca gaaattccag tgcgacaaag atgagcagca gattctgcgt
     8221 cgcgacgaga aacatcatcg ccacggtggc aaacacgatc agggccagga attcgcccac
     8281 atgctcggtg atccgcgtgg atgttgacag cagaacggtc agaattccca gcgcaagcag
     8341 ggccatctgc accacacgtg tcagcggtgt gacgacgaac atcgcatccg gaaggctcat
     8401 cgttccggtg ccatgcatca gcatccagat cgccaccagc gacccgatac aggcgatcag
     8461 cgcaccggcc ccgaatcgcc gtcgggccgc gccccggcgc atcaccgata gatccagcgc
     8521 cagcagaaca aggcccgtca ccgccaccac aatctcgggc gaggtcgctc gcagcagttc
     8581 ggcgtagctg atcgcgttca tcgccacgct ccctgccgca agccttcaaa cagcggcgag
     8641 ttcagcgcgg gcagaataat atccgtcaga acgcggggat agagtcccac ggcgagcgtg
     8701 ctcgccagca gcagcagtgc cgccagcttt tcaggggacg aaatcggcgg caggacgtgt
     8761 tcggccgcgc ccggagtcgg gtcgccgtag aacgccttcg tcagcgcgcg ccaggtgaac
     8821 gcgatcccca ccagaatgcc gatgccggcg atcacaacca tccacgagaa cgaccgccat
     8881 gtccccacca gcacctggaa ctccgcgatg aacccgctga atcccggcag cccgatcgac
     8941 gccatgccgg ccagaatgaa cgtagccgcc gcgaagggaa tcgcgcgtcc cagctgcatc
     9001 ggtcccagtt cgttcagatc gcgcgtgtgc gtgcggtcgt agaccatgcg tcccacaacc
     9061 gcaaagagca gacccgcgat cacgccgtgg gaaaacatct gcagcaccgc gccgtcgagc
     9121 ccgatttgcg tcagcgtcat caatcccagc agcacaaaac ccatgtgcga aacgctggag
     9181 tatccgatga caaacttgaa atcgcgctgc accagggcca cactggcgcc atagacaatg
     9241 ccgatcaccg caagcagcgc gaagacttcg cgccatgacc ccagacccag cacgtggaaa
     9301 ccccagggac ccatgccggc agggaagagc atcatcgcaa cgcgcaggca gccgtaagcg
     9361 cccagcttca tcaccacgcc ggccagcagc atcgaagccg cagtcggggc cgcggcgtgt
     9421 ccggtcggcg cccaggtgtg gaagggccac atgccggcaa gcaccgcaaa gcccacgaac
     9481 acgagcggga agagagccat ctgcagcgag gttgaaagat gcgctgccgt aagcgtatcg
     9541 aagctcatgc tgtggccgcc cgtcgctacc cacgtcgcca ccatgcccgc caaaaccagt
     9601 gcgctgccgc cgaaggaata aagcgccagc ttcatcgcag cgtactcacg ctgcgacccg
     9661 gcttcttcgc ccgtcgcacc tgccttcgac gatccccaga tcgcgatcag aaaatatttc
     9721 ggaataatcg caatttcgta aaacacaaac agcagaaaca gatcgaagct aagaaataca
     9781 ccgtagacgc caccgatcag caccaggtag aaagcgaaga actcctgggt ccgcttttcg
     9841 atgttccacg agaagagaat ccccgccgtc gccgcaagtc cggtcagcag caccagcacc
     9901 aggctgatgc cgtccgctgc
//
LOCUS       DASZYN010000039         3355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1094184, whole genome shotgun sequence.
ACCESSION   DASZYN010000039 DASZYN010000000
VERSION     DASZYN010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094184"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..204)
                     /locus_tag="VHE33_01130"
     CDS             complement(34..204)
                     /locus_tag="VHE33_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96077.1"
                     /translation="MTTGGLTEEMLALAEYLYLMAVARGETKLSAYHEVSGASARLER
                     GWSAEDKRKWLT"
     gene            complement(201..1448)
                     /locus_tag="VHE33_01135"
     CDS             complement(201..1448)
                     /locus_tag="VHE33_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96078.1"
                     /translation="MQGLTGRLIFVLAAAMAVAGCSSTSKAAAAGSESSGAVPAPGGK
                     AGPANYVKVALTSQTTTATIADPSLDNITAATLTIPAGWKLQGIEMKSPCTFVPWPVF
                     RAYSPDGLLQYRIEPVIGWQWNPSGRQYNSGCAKISGVMSAANFLQYYLGTMQGGVHV
                     VGPMTVPAAYSQWAQGLAAQANQMNARVAPALRASNTADTAALRIEVVNGSFVVEERV
                     RTAVECADNRNAGPLNGGTCWARVDVLTAPQGQLDALVKVVDTENLPHGVNTQQWIQA
                     ALARQQREGQRQIDQLTAQEKAESQMLYSQFQQSMQRMQQEHEQFMAQQESQFESSMN
                     NATAAMNARTTAASDWVDYALDQKTVENPNGTITKVSNAYSQTWTNGTQWYQTNDPNV
                     NPNGVLQGNWTLTTQVHGNGEAQ"
     gene            complement(1470..2726)
                     /locus_tag="VHE33_01140"
     CDS             complement(1470..2726)
                     /locus_tag="VHE33_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96079.1"
                     /translation="MKRGSYIFASVVAAAILTAGCNSGAKAASTGDGSGAPQSAQKAA
                     GSPGAVKYVSGTEPSNSTATATLLDPTLNNMPAATLTVPEGWKTQGMMMTSPCTTLPV
                     AVFRSYSPDGLMQMRAEPAMGWAWTTEQKMATVRGCLPLQKPVTAAQFLDAYIGMIQG
                     GVHVVGAIELPQDQHQALAQRIAQLNQMEAQSPVMAGITNSGDMSAVRIETVNGSFVI
                     EERLRATVMCQLNNNPGFQHGGTCFAQVDQLMAPKGRLDGLMRTMDSQHLPRAKASDQ
                     WFQAMMQQRQRQWDEFGRKLRALEQQESEMLAQQAQQFSQRMASDHAAYMQQQESEYQ
                     SERTNFNAQMNAKSTAASDWVDYALDRQTVAVGGTTAKISSAYSQTWSNGSQWYQTND
                     PNANPNGVLPGNWTLATQVHGNGQPK"
BASE COUNT          521 a         1201 c          898 g          735 t
ORIGIN      
        1 ccttgtcgtt cagcgccggg ttcgaagttc tcatcaggtc agccacttcc tcttgtcctc
       61 cgcggaccac ccccgctcca accgcgcaga agccccgctg acctcatgat atgcgctcag
      121 cttcgtctct ccccgcgcca cggccatcag atacaaatac tcggccagcg ccagcatctc
      181 ctcggtcagt cctcccgtcg tcattgcgcc tctccgttcc cgtggacctg cgtcgtcagc
      241 gtccagttgc cctgcagcac gccattcgga ttcacgttcg ggtcgtttgt ctgataccac
      301 tgcgtcccgt ttgtccaggt ctgcgagtac gcattcgaga ccttcgtgat ggtgccgttc
      361 gggttttcaa ccgtcttctg atccagtgcg taatccaccc agtccgatgc cgccgtcgtc
      421 cgcgcgttca tcgcggcggt cgcattgttc atcgaactct cgaactgcga ctcctgctgc
      481 gccatgaact gctcgtgctc ctgctgcatc cgctgcatcg attgctgaaa ctggctgtac
      541 agcatctggc tctctgcctt ttcctgtgcc gtcagctgat cgatctgcct ctgcccctcg
      601 cgctgctgcc gcgccagcgc tgcctgtatc cactgctgcg tgttgactcc gtgaggaagg
      661 ttttccgtgt ccaccacctt caccagagca tccagctgcc cctggggcgc cgtcagcaca
      721 tccacccgcg cccagcacgt cccgccgttc agcggcccgg cattccggtt gtcggcacat
      781 tccacagctg tccgcacccg ttcctcgacc acaaagctac cgttcaccac ttcgatccgc
      841 agagccgccg tatccgcggt gttgctggct cgcagcgccg gagccaccct tgcattcatc
      901 tggttcgcct gcgccgccag cccctgcgcc cactgcgagt acgccgccgg caccgtcatg
      961 ggccccacca cgtgtacacc gccctgcatc gttcccagat agtactgaag aaaattggcc
     1021 gccgacatca cgccggaaat cttcgcgcac ccgctgttgt attgtcgtcc gctcgggttc
     1081 cactgccagc cgatcaccgg ctcgatgcgg tactgcagca gtccatccgg cgagtacgcc
     1141 cgaaacaccg gccacggaac aaatgtgcag ggactcttca tctcgatacc ctgcagcttc
     1201 catcccgccg ggatggtcag cgtcgccgcc gtaatattgt ccagcgatgg atccgcgatt
     1261 gtggccgtcg tcgtctgact cgtgagcgcc accttcacat agttcgccgg tcctgccttt
     1321 cctcccggcg ccggaaccgc tcccgaactt tcggatcccg ccgctgccgc tttcgatgtg
     1381 gaagagcaac ccgccaccgc catcgccgcc gcgagaacaa agatcagcct accggtcagt
     1441 ccctgcatga tgtccctctc cccttcggat tacttcggtt gtccgttgcc atggacctgg
     1501 gtcgccaggg tccagttccc cggcaatacg ccgttcggat tcgcgttcgg atcgttggtc
     1561 tggtaccact ggcttccgtt cgaccatgtc tgcgagtaag cgctggatat cttcgccgtc
     1621 gtgcctccca cggccacggt ttgccgatcg agcgcgtagt ccacccagtc ggaggccgcg
     1681 gtactctttg cgttcatctg cgcgttgaag ttggtcctct cgctttgata ctccgactcc
     1741 tgctgctgca tgtacgcggc gtggtccgac gccatcctct ggctgaactg ctgggcctgc
     1801 tgcgccagca tctcgctttc ctgctgctcg agcgccctta gcttcctgcc aaactcgtcc
     1861 cactgcctct gccgctgctg catcatggcc tggaaccact ggtctgacgc ctttgctcgc
     1921 ggcagatgct ggctgtccat ggtgcgcatc aacccatcca gccgcccttt cggcgccatc
     1981 agctgatcca cctgggcgaa gcatgtgccg ccatgctgga atccgggatt gttgttgagc
     2041 tggcacatca ccgtcgcgcg cagccgctcc tcgatcacga agcttccgtt cacggtctcg
     2101 atccgcaccg cgctcatgtc gccgctgttc gtgatgccgg ccatcaccgg cgactgcgcc
     2161 tccatttgat tcagttgtgc gattctctgc gccagtgcct gatgctgatc ctggggcaac
     2221 tcaatcgccc ccaccacatg aacaccaccc tgaatcatgc cgatataggc gtccagaaac
     2281 tgcgccgccg tcactggctt ctgtagcggc agacatcccc gaaccgttgc catcttctgt
     2341 tcggtggtcc acgcccagcc catcgccggc tcggctcgca tctgcatcag cccgtccggc
     2401 gaatagctgc ggaagaccgc aaccggcagc gtcgtacagg gactcgtcat catcatgccc
     2461 tgcgtcttcc atccctccgg aaccgtcagg gtggccgccg gcatgttgtt gagcgtgggg
     2521 tcgagaaggg tcgccgttgc tgtggagttc gacggctccg tgccgctgac gtactttacc
     2581 gctcctggcg aaccggcagc cttttgcgcg gactggggcg ctcccgatcc gtcaccggtt
     2641 gacgccgctt ttgctcccga gttgcatccg gcagtcagga ttgctgccgc aacaacactc
     2701 gcaaagatat agcttcctct cttcatatct ctccctggcg gttcgttgac ggcgctccgc
     2761 gttgccgtgc gccccagtgg caccgtccag gtgccctgcg gaacgtcttt tggatttgca
     2821 tgtgcatcat gggtacgatc agctcccgat cacccaggtt cgcggctggt cgcaaaaccc
     2881 gcgtggcaga cccgtctgcg cgatcggaaa ggtgacgaca ccgggctcgc tggtccgcgg
     2941 ctggctttca ccgcgtccag cgtcagcgct ccgcctgtcc cggccgcacg ccgctctgtg
     3001 cccggcctcc cggactccgc gccagagctc gcgcattacg gcgtagcgga cgaagaaaca
     3061 cagaaagcgc aatgagtgaa agaaccatca gagaaatatc cggcagaaga cccatgcgtg
     3121 ccctcccttt gcaacaccat gtcggactcg agcgccgttt ccggggagaa ttagcgaatt
     3181 cgccgagaat ggtttctgtg caccctatgc tgggaaaaat tcgcggcggc ctcgctggac
     3241 tttcggccgc cgcttcaagg tgcggattta ccctcgcacg ccccttccgc cttcagccag
     3301 cggcatgcaa ggtcaactgg ggacaagtcg tgacatccgc cgttccggtc cgccc
//
LOCUS       DASZYN010000040         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_837291, whole genome shotgun sequence.
ACCESSION   DASZYN010000040 DASZYN010000000
VERSION     DASZYN010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837291"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VHE33_01145"
     CDS             complement(<1..992)
                     /locus_tag="VHE33_01145"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HVT96080.1"
                     /translation="MAPGHHERLSVVFATSDDGALTDLGQELAPAFFINIAPDSDALF
                     RTLSEQAPEIVVIDLDTIVPRGADVFEWTASVRAVAAGTLLIIISRTPLKSARQRAKK
                     AGADEFLLAPVDFAELREFLLEAGEERRARTEAQQLREGISQKTSFCGMIGGSEAMQR
                     VYEALRRVAPSNTTVMLRGESGTGKELAARAIVSLSPRREGPFISVNCAALPETLMES
                     ELFGHEKGSFTGAHATRPGQVELADGGTLFLDEIGSLGLALQSKLLRVLQDRAVQRIG
                     GKSPRKIDFRLITATNDDLEDMVKKGQFREDLYYRICVVPIQLPPLREREGDVPL"
     gene            complement(1068..>1764)
                     /locus_tag="VHE33_01150"
     CDS             complement(1068..>1764)
                     /locus_tag="VHE33_01150"
                     /inference="COORDINATES: protein motif:HMM:NF044456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5110 domain-containing protein"
                     /protein_id="HVT96081.1"
                     /translation="LGPNWPPEQELHDPQVEEICREYLNLRYRLLPYLYSSVAQAHAT
                     GLPLIRPLWLAHPEDKHALTREDEYLFGDSFLVAPVLQAGVDKRAVYLPAGHWWDFWT
                     EESTQGERESSRTVDLKTLPLYVKAGSVVPFGPVRQYTTEPVDEPLTLRIYPGANGSF
                     ALYEDDGESFRYEQGEFTRIFCEWNDQERKLSLRVDPKGKPATGKKLRVEIAGSKSGK
                     KVTLRGALTTVEL"
BASE COUNT          308 a          556 c          549 g          351 t
ORIGIN      
        1 agggggacat cgccctcgcg ctcgcgcagc ggcggcagct gaatgggcac gacgcagatg
       61 cggtaataga ggtcttcgcg gaactggccc ttcttcacca tgtcttccag atcgtcgttg
      121 gttgcggtga tgaggcggaa atcgattttt cgcggagatt tgcctccgat tctctgaacc
      181 gcgcgatcct gcaagacccg cagcagcttg ctttgcagcg ccagaccgag ggatccgatc
      241 tcatcgagga aaagcgtgcc tccgtcggcc agctcgacct ggcctgggcg cgtggcgtgc
      301 gctccggtga acgatccctt ttcgtgcccg aacagttcgg attccatcaa agtctcgggc
      361 agtgctgcgc agttcacgct gatgaagggg ccctcgcgcc ttggactgag agagacaatc
      421 gcgcgcgcgg cgagttcctt gcccgttccg ctttcgcccc gcagcatcac cgttgtattg
      481 ctcggcgcca cccgccgcag cgcctcgtag acgcgctgca tggcctcact gccgccgatc
      541 atcccgcaga acgaggtctt ctggctgatg ccctcgcgca gttgctgcgc ctctgtcctg
      601 gctctgcgct cctcgcccgc ctcgaggaga aattcccgca actcggcgaa gtccacggga
      661 gccagcagga actcgtcggc tccggctttc ttcgcccttt gccgggcgct cttcagcggc
      721 gtgcgcgaga tgatgatgag cagggttccc gcggccaccg cgcgcacgga cgcggtccac
      781 tcgaaaacat cggcgccccg ggggacgatc gtatccagat cgatgacgac gatctccgga
      841 gcctgttccg acagcgtgcg gaacagagcg tctgaatccg gagcaatgtt gatgaagaac
      901 gcgggggcca gttcctgtcc cagatccgtc agtgcgccgt cgtcactggt tgcaaataca
      961 acgctgagcc gttcgtggtg gcccggtgcc atcgcgactc ccgtgctgca aagttggtct
     1021 tctgggggtg atcgagatta tagcgacaac tttgcgggac gctgccatca aagctcgacg
     1081 gtggtcagcg cgcctcggag ggtcaccttt ttcccactct tcgagcccgc aatctctacc
     1141 cgcagctttt tgccggtcgc cggcttccct ttcggatcca cgcgcagcga cagctttcgc
     1201 tcctgatcgt tccactcgca gaagatccgc gtgaactcac cctgctcgta gcggaagctt
     1261 tcaccgtcgt cttcatagag agcgaagctg ccattggcgc caggataaat gcgcagcgtg
     1321 agaggctcat cgaccggctc ggtggtgtat tgccggaccg ggccgaaagg aacgacggag
     1381 cctgccttca cgtagagcgg gagcgttttc agatcgaccg ttcgcgagga ttcgcgctcg
     1441 ccctgcgtcg attcctctgt ccagaaatcc caccaatggc cggcgggcag atagacagcg
     1501 cgcttgtcta cgccggcctg cagcacgggt gcgacgagaa aactgtcgcc gaagagatat
     1561 tcgtcctcgc gtgtcagcgc gtgcttgtcc tcgggatgag ccagccacag cggccggatt
     1621 agaggcaggc cggttgcatg cgcctgcgcg acggacgaat agagataagg aagcaggcgg
     1681 tagcggaggt tgagatactc gcgacaaatt tcctcgacct gcgggtcgtg cagttcctgt
     1741 tcgggcggcc agttggggcc cagc
//
LOCUS       DASZYN010000041         7467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_708861, whole genome shotgun sequence.
ACCESSION   DASZYN010000041 DASZYN010000000
VERSION     DASZYN010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7467
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708861"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VHE33_01155"
     CDS             complement(<1..359)
                     /locus_tag="VHE33_01155"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HVT96082.1"
                     /translation="MSSKEDPRLSRIAAICSDLPESARANMGDHAQFTVRKKTFAYFL
                     NNHHGDGIVSICTRAFPGENKKLIEAHPRKFYLPAYVGPRGWVALRLDRGKVDWDEVR
                     DLITASYLQAAPKKLAAL"
     gene            complement(492..1253)
                     /locus_tag="VHE33_01160"
     CDS             complement(492..1253)
                     /locus_tag="VHE33_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HVT96083.1"
                     /translation="MRPLTRRKLITGGAITAAVGVTGLAAADRIARRWGLIPPDSSGL
                     YGAGETLTYAAQRVIGKHALAREFSRGMISKTPFMNEVAPLDDAFKKHQAAGFRDWRL
                     VVDGMVEHPLSLSVSDLQALAKQNQITEVVCEEGWSYIAEWIGTPLTAVLQEAGTLPK
                     VRYIVYRSIQTDWWESIDMADALHPQTFLTWGMNDGELPVGFGGPLRMRIPRQLGYKS
                     LKYITHLTATDSMKKFGKGLGSSSPEAGYAWYAGI"
     gene            complement(1250..1975)
                     /locus_tag="VHE33_01165"
     CDS             complement(1250..1975)
                     /locus_tag="VHE33_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HVT96084.1"
                     /translation="MATAVSQPGVAAPRHTVWVRVTHWLTVVAFLALAFSGFMIILTH
                     PRFYWGETGNVNMKPLFRIPVPSSRDTVPTGYNYVLADENGWARSLHFEAAWLFVLTG
                     AVYGIAGFASGHFRRNLVPARGERSLASYGRALRGIETNEDGAVTYNVVQRTAYLSVL
                     FILTPAVIWTGLALSPAFNAAFPFFVDVLGGRQSARTLHFFVSDLLVVFLVVHVLMIA
                     LAGFWTRTRAMITGRAKNAEDNA"
     gene            complement(2051..3097)
                     /locus_tag="VHE33_01170"
     CDS             complement(2051..3097)
                     /locus_tag="VHE33_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96085.1"
                     /translation="MPAISAADTISPAIQRTKAFLFRPFSLGTYLKLCLVAVLTGGSS
                     GGSGNFHVPGGGHTPSHPSHSYAALPHFAFTPERIAAAVAIVALLIIIGLFLSWLIMR
                     LRFAYFHCLSTNTRLIRTGWHLYRGPATRFFWFNVVVGLCFLVLMAALALPFAAGFLG
                     LFHGIRAGGHPNIGAIIALVLPLIPIILLIVILAIVVKIVLLDFMLPHFALENASAGQ
                     AWSAVWARIRYEKGGFFVYALLRVLLPIVAAICIVIVLFIPAILCVLAVVGIEVGLHA
                     ALGHTALAILLQVCVGVVACAVAILAFIAAFGPLETAVREYALIFYGNRYRLLGDILY
                     PPSPAAAIPTPVNP"
     gene            3265..3810
                     /locus_tag="VHE33_01175"
     CDS             3265..3810
                     /locus_tag="VHE33_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVT96086.1"
                     /translation="MRLGEKLKYLREAEGELRGLGRAITQQEMVRALKTETGKALSQS
                     YLSQIESGARPHLTNTTRQVLAKFFKVHPGYLVDDPEGYSAELLSDARTLEDKLDLWL
                     VNGAERFRRDAELKQSLLALARHEDSRRCLLLLNAVIDTPGLVDRLFEVLGSGSDNGH
                     GTVAKVRPAKTGPMRRKGAAQ"
     gene            3807..4433
                     /locus_tag="VHE33_01180"
     CDS             3807..4433
                     /locus_tag="VHE33_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96087.1"
                     /translation="MITFYLICFTLGLSLTLLSALGVFTHLHFGHFHFGHAHTHVAHV
                     PGGVKSGVSPVNGFTLAAFLCWFGGCGYLLARSGDFGMAVVLAIATATGLAGGAILFW
                     FLVKVLLPHEHELTAADTDILGILGQVTGTIREGGTGEIQFRQNGSRHFAPARSDAGV
                     AIARGIEVVVMRYDQGIAYVRPWEELSGESLEISAGSSTANATPTQQP"
     gene            4461..5900
                     /locus_tag="VHE33_01185"
     CDS             4461..5900
                     /locus_tag="VHE33_01185"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HVT96088.1"
                     /translation="MTNTIIIIVGLSLLAFIILMGMLARMFRKAGPNEALIVYGFRGP
                     RVIKGHGTIIFPMVENCRELSLELMSFDVAPQQDLYTKQGVAVTVEAVAQIKVRSDQE
                     SILTAAEQFLTKTPPQREGLIRLVMEGHLRGIIGQLTVEQIVKEPEMVADRMRGTCAD
                     DMSKMGLEVVSFTIKEVRDKNEYITNMGRPDIVRIKRDAEIAAAEAERDTAIRRANAT
                     REAAVAKAAADQERVIAETASLSKQAQAQRDLDISKAQFAEQSRKQQASADKAYELQT
                     NITQQQVVAEQVKVQQVEKEQQIKVQEAEILRHEKELIATVLKEAEVNRQRVEVLAAA
                     EKARLTMEAEGRAAAIQTQGQAEASIIFQKGEAEAKAMNVKAEAYQEWSQAAVVDRLI
                     SNLPEVVRAMSEPLSKVDKITIVSTGGDAAGANKITGDMAKMAAQVPALFEALSGMDL
                     RQLMSNITAMRQRPNGNGAAGEGPVVDAR"
     gene            5982..6650
                     /locus_tag="VHE33_01190"
     CDS             5982..6650
                     /locus_tag="VHE33_01190"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HVT96089.1"
                     /translation="MALLERVSTLLRANLNDLIDKAEDPEKLLKQLVLDMENQLMQVK
                     TQVAIALADEHLLEKKRREHEDSEADWRRKAELAVRKGDDNLARAALERSLSAKGMAA
                     GFAQQLEDQKTEVETLKTALRNLEQKLQETRSRCELMIAESRRARVVGRAQKAREAIA
                     RKEGDVSITRMKHRIAGAEAQNAAAREMMDGDGVEAKFAKLEREEQIDALLRDLKEQH
                     AKTA"
     gene            complement(6779..7060)
                     /locus_tag="VHE33_01195"
     CDS             complement(6779..7060)
                     /locus_tag="VHE33_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96090.1"
                     /translation="MNKSDKPIVRIENWSVVGSVIYYGFRRLELGARLTGDVMGHANL
                     RNGTIYTSAILSIDRLKNHVETHNSIYQLGEVSDEYARWIEAQEKPQAA"
BASE COUNT         1560 a         2093 c         2550 g         1264 t
ORIGIN      
        1 agcgcggcca gtttctttgg agcggcctgg agatagctcg ctgtgatcag gtcgcgcact
       61 tcatcccagt cgactttgcc gcggtcgagg cgcagggcga cccatccgcg cggaccaacg
      121 taggcgggga gatagaactt cctgggatgc gcctcgatca gtttcttgtt ttcgcccggg
      181 aatgcccgag tgcagatcga gacgatgcca tcgccgtggt ggttgttgag gaaataagcg
      241 aaggtctttt tgcggacggt gaactgcgca tgatcgccca tgtttgcgcg ggcggattcc
      301 gggaggtcgg agcagatcgc ggcgatccgg ctgagccgag ggtcctcttt gctgctcatc
      361 cggccctccc tctgaaagac ccgctaaaaa tccacgaccg gctaccaatt cgcaggagcc
      421 ctcgtacgcc tcgtgttgcc gtaaccctga ccttcatgcg tcacatctga tcgcccgccg
      481 ccagagcgat ttcaaatccc ggcgtaccag gcgtagccgg cttcaggcga ggaggagccg
      541 aggcccttgc cgaatttctt catggaatcg gtcgcggtga ggtgggtgat gtacttcagg
      601 ctcttgtacc cgagctggcg cgggatgcgc atgcgcagcg gaccgccgaa gccgacgggc
      661 agctcgccgt cgttcatccc ccaggtgagg aaggtttgcg gatggagtgc gtcggccatg
      721 tcgatgctct cccaccagtc ggtctggatg gagcgataga cgatgtagcg gaccttgggc
      781 agcgtgccgg cttcctggag gacagccgtg agcggcgtcc caatccactc ggcgatgtag
      841 gaccagcctt cttcgcagac gacttcggtg atctggttct gtttcgcgag tgcctggaga
      901 tcggagacgg aaagcgagag cgggtgctcg accatgccat cgaccacgag gcgccagtcg
      961 cggaagccgg ccgcctggtg cttcttgaac gcatcgtcca gaggcgcgac ttcgttcatg
     1021 aacggcgttt tcgagatcat gccgcgggag aattcacgag ccagagcgtg tttgccgatc
     1081 acccgctggg cggcgtaggt aagcgtttcg ccggccccgt agaggccgct gctgtcgggc
     1141 gggatgagtc cccagcggcg tgcgatgcga tcagcggcgg cgaggcccgt cactcccaca
     1201 gcggctgtga tggcgcctcc cgtaatgagt tttcggcgtg tgaggggcct catgcgttgt
     1261 cctccgcgtt tttggctcgg ccggtgatca tggcgcgggt gcgcgtccag aatccggcga
     1321 gggcgatcat cagcacgtga acgacgagaa acacgacgag cagatcggag acgaagaagt
     1381 ggagagttcg cgccgactgg cgtccgccga gcacgtcaac aaaaaatggg aaagcagcat
     1441 tgaacgctgg tgagagcgcg aggcccgtcc agatcacggc cggcgtcagg atgaagagga
     1501 cgctgagata agcggtgcgc tggacgacgt tgtaggtgac ggcgccgtcc tcgttcgtct
     1561 caataccgcg taaggcgcgc ccgtaggagg ccaaggaccg ctcgccgcgc gccgggacca
     1621 ggttgcggcg gaagtgtccg gaagcgaagc cggcgatgcc gtagaccgcg cccgtcagga
     1681 cgaacagcca tgcggcttca aagtggaggg accgggccca gccattttcg tctgccagca
     1741 cgtagttgta gccggtcgga accgtgtcac gggaagaggg cacggggatg cggaacagcg
     1801 gcttcatgtt gacgttgccg gtttcgcccc agtagaagcg cgggtgcgtg aggataatca
     1861 tgaagccgct gaaagccagc gccaggaagg caacgacggt gagccagtgc gtgacccgga
     1921 cccatacggt gtgtcgcggg gctgccactc cgggttggga aaccgcggtc gccatgcggg
     1981 ggaggatagc acggcgagcc ggcgacagaa catatcaact gaagaaagca gcctggggac
     2041 cgacggcccc tcaggggttg accggcgtcg gaatggcagc ggccggcgac ggcggataga
     2101 gaatgtcgcc cagcagtctg tagcgattgc cgtagaagat cagcgcgtat tcacgaaccg
     2161 ccgtctctaa cggcccgaag gcggcgatga acgccaggat ggcgacggcg caggccacga
     2221 ctcccacgca gacctggaga aggatggcta gggctgtgtg tccgagggcg gcgtgcaggc
     2281 ccacttcgat gccgaccacc gcaagaacgc acaggatcgc cggaatgaac agaacgatca
     2341 cgatacagat ggcggcgacg atggggagca gcacccgcag gagggcgtag acgaagaatc
     2401 ctcctttttc atagcgaata cgcgcccaga cagcggacca ggcctggccc gcgctggcgt
     2461 tctcgagggc gaagtgggga agcatgaagt ccagaagcac aatcttcacc acaatggcca
     2521 gaatcacgat gagcagaatg atcggaatca gcggcagcac aagagcaatg atggcgccga
     2581 tattcggatg tcccccggca cggatgccgt ggaacagtcc caggaagccc gcggcgaagg
     2641 gcagagcgag ggcggccatg agcacgagga agcacaggcc gaccacgacg ttgaaccaga
     2701 aaaagcgtgt cgcgggtccg cgatagagat gccagccggt gcgaatgagt cgcgtgttcg
     2761 tgctgaggca gtggaagtac gcgaagcgca ggcgcatgat gagccaggag aggaacagac
     2821 cgatgatgat cagcagggcg acgatcgcaa cggccgcggc gatgcgctcc ggcgtgaagg
     2881 cgaagtgcgg gagcgccgca tacgagtggc tgggatgact cggggtgtgg ccgcccccgg
     2941 gcacgtgaaa attgccgctt ccgccagatg agcctccggt gaggacagcg acgaggcaga
     3001 gcttgaggta ggttccgaga ctgaagggcc ggaagaggaa agccttcgtt cgctgaatcg
     3061 cgggcgaaat ggtgtcggct gcagagatgg cgggcatagg aagccccttt cggttgcgaa
     3121 gtgtactgca aaagcggccg gagcggcggg ctgtgaatcg gtctgacaaa cccctgacag
     3181 caaagcccgc aaccggcccc gtttcgattt cgcttgacta tatcacggat tgcgatatag
     3241 tgattgcgat ttgcaatatt tcttatgaga ctcggtgaaa agctgaaata cctccgggaa
     3301 gctgaaggcg agctgcgcgg gctggggcgc gccatcaccc agcaggagat ggtccgcgcc
     3361 ctcaagactg aaaccggcaa ggccctgagc cagagttacc tgtcgcagat cgaaagcggc
     3421 gcgcggcccc acctgacgaa caccacgcgc caggtgctgg caaagttctt caaggttcat
     3481 cccgggtatc tggtggacga tccggaggga tatagcgcgg aactgctgag cgacgcgcgc
     3541 acgctggagg acaagctgga cctgtggctg gtcaacggtg cggagcggtt ccggcgcgac
     3601 gcggagctga agcagtcgtt gctggcgctg gcgcggcacg aggattcgcg gcggtgtttg
     3661 ctgctgctca atgctgtgat cgacacgccg gggctggtgg atcgcttgtt tgaagtgctg
     3721 ggttcgggga gcgacaacgg gcacggaacc gtggcgaagg tgcgtcccgc aaaaaccggt
     3781 cccatgcgca ggaaaggagc cgcccaatga tcacgttcta tctgatttgc ttcacgctgg
     3841 gcctttcgct gaccttgtta tcggcactgg gggtcttcac ccatctgcat ttcgggcact
     3901 ttcatttcgg tcatgcgcac acacacgtgg cgcatgtacc gggcggcgtg aagtccggcg
     3961 tgagcccggt caacggattc accctggcgg ccttcctgtg ctggttcggc ggatgcggct
     4021 atctgctggc gaggagcggc gacttcggca tggcggtggt cctggcgatc gcgacggcaa
     4081 ccggcctcgc cggcggagcg attctgtttt ggtttctggt gaaggtgctg ctgccgcacg
     4141 agcacgagct gaccgcggcg gataccgaca tcctgggaat cctcggccag gtcaccggga
     4201 cgatccggga aggcggcacc ggcgaaattc agttcaggca gaatggcagc cgccattttg
     4261 cacccgcgcg cagtgatgca ggcgtagcga ttgcgcgcgg aatcgaagtg gtggtgatgc
     4321 gctacgacca gggcatcgcg tacgtgcgtc cgtgggaaga attgtccggc gaaagcctgg
     4381 aaatttccgc gggcagctcg accgcgaacg ccaccccaac ccaacaaccg taacagcccc
     4441 gatggaaagg atcgggcgtt atgacgaaca caatcatcat tattgtcgga ttaagcctgc
     4501 tggcattcat catcctgatg ggcatgctcg ccaggatgtt tcgcaaggcg ggccccaacg
     4561 aggcgctcat cgtatatgga ttccgcggcc cgcgggtcat caagggccac ggcacgatca
     4621 tcttccccat ggtcgagaac tgcagagagc tttccctgga gctgatgtcg tttgacgtcg
     4681 ctccccagca ggatctgtat acgaaacaag gcgtcgcggt gaccgtcgaa gccgtggcgc
     4741 agatcaaagt gcgcagcgat caggagtcga tcctgaccgc ggcagagcag ttcctcacca
     4801 agacgcctcc ccagcgcgaa ggcctgatcc ggctggtgat ggaaggccat ctgcgcggca
     4861 tcatcggcca gctcaccgtc gagcagatcg tgaaggaacc ggagatggtc gccgaccgta
     4921 tgcgcggaac ctgcgccgat gacatgagca agatgggact cgaggtggtg tcgttcacca
     4981 taaaagaagt ccgcgacaag aatgaataca tcaccaacat ggggcgcccg gacatcgtgc
     5041 gcatcaagag agacgcggaa atcgcggcag ccgaggcgga gcgcgacacg gccatccggc
     5101 gcgcgaatgc gacgcgggaa gcagccgtcg cgaaggctgc ggccgatcag gagcgggtga
     5161 tcgcggagac ggcctcgctc agcaagcagg cgcaggccca gcgggatctg gacatctcca
     5221 aggcgcagtt tgccgagcag agccgcaagc agcaggcttc ggcggacaag gcctacgagc
     5281 tgcagaccaa catcacgcag cagcaggtcg tcgccgaaca ggtgaaagtg cagcaggtgg
     5341 agaaagagca gcagatcaag gtgcaggaag ccgagattct gcgccatgag aaggagctga
     5401 tcgccaccgt gctgaaagaa gccgaggtca accggcagcg cgtcgaagta ctggcggctg
     5461 ccgagaaggc gcggctgacg atggaggccg agggccgcgc ggccgcgatt cagacccagg
     5521 gccaggccga ggcttcgatc atcttccaga agggcgaggc cgaagcgaag gccatgaacg
     5581 tcaaggccga ggcttaccag gaatggagcc aggccgccgt ggtcgaccgc ctcatctcga
     5641 acctgcccga agtggtgcgg gcaatgtcgg agccgctgtc gaaggtggac aagatcacca
     5701 tcgtgtccac cggcggagat gcagccggcg cgaacaagat caccggcgac atggcgaaaa
     5761 tggcggcgca ggtgccggct ctcttcgaag cgctgtcggg aatggatctg cggcagctga
     5821 tgtcgaacat taccgccatg cgccagcggc caaacggcaa cggagctgcg ggtgagggtc
     5881 cggtggtcga cgccaggtaa ggaaacacca gggcggcgcg aacgatcgct ccgccccggc
     5941 tggaaagttt cgaacggaat tcagaattgg agggaaatgt catggcactc ctcgaacggg
     6001 taagcacgtt gctgagggca aatctgaacg acctgatcga taaggcggag gatccggaga
     6061 agctgctgaa gcagctggtc ctggatatgg agaaccagct gatgcaggtg aagacccagg
     6121 tggcgatcgc cctggcggac gagcacctcc tggagaagaa gcgccgggag cacgaagatt
     6181 ccgaagccga ctggcggcgc aaggccgagc tggccgtgcg gaagggggat gacaatctcg
     6241 cgcgagcggc actggagcgg tccctgagcg cgaaagggat ggctgccggc tttgcacaac
     6301 agctcgaaga tcagaagacc gaggtggaga ctttgaagac ggcgttgcgc aatctggaac
     6361 agaaactcca ggagacgcgg agccgctgtg aactgatgat tgccgagagc cggcgggcgc
     6421 gggtcgtggg acgcgcgcaa aaggcgcgcg aagcgatcgc ccggaaggaa ggggatgtca
     6481 gcatcacgcg gatgaagcat cgcattgccg gagccgaggc gcagaatgct gccgcgcgcg
     6541 agatgatgga cggcgatggc gtcgaagcga agttcgcgaa gctggagcgc gaggaacaaa
     6601 tcgatgcgct gctccgagac ctcaaggagc agcacgcgaa gacggcttga gtgcggatcg
     6661 aagccgcacc agaaccgtga acaaagggcg attggcaaag ctcttcgcga gcgatcttcg
     6721 gatcgctcgt cactgacaga ttttgccggg agctgtttgc tgatcgccct tcgcggcttc
     6781 aggcggcctg gggtttttcc tgtgcctcga tccagcgggc gtactcgtcg cttacttcgc
     6841 cgagttggta gatgctgttg tgggtctcga cgtggttttt caggcggtcg atgctgagga
     6901 tcgctgacgt gtagatggtc ccgttgcgga ggttggcatg gcccatcacg tcgccagtaa
     6961 gacgggcgcc caactcaagt cgacggaagc cataatagat aacgcttccg acgacagacc
     7021 agttctcgat gcggacgatc ggcttgtcgg acttgttcat tttttcgtga cctgacgtat
     7081 gccgccaccg gtcgcgttca acggtccaag cctgtggctg gtgcccttcc agaaaatgga
     7141 aagcaattgt ggtgccatcg taacgcttga gtaaatagcg tcatttagtg acaacggcgg
     7201 gtggcgctgt gaaatttttt cccagtcagg gaagattgtt gcctgttcgc gcccgcgctg
     7261 gagcgttagt cacaggggcc gtttcccagg ccggtggttc tatatactgc cggtcatgct
     7321 ctgcaaacct gaatcattcg cattctccgc cactcgtgtg cctgccttga tgatcctcgc
     7381 cggggcaatg acgctggcac ctgcggcgtc ccatgcgcaa tctcccgcca ctgtgcaagc
     7441 gcctcatccc atgaatttgg acgactt
//
LOCUS       DASZYN010000042        10515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_837437, whole genome shotgun sequence.
ACCESSION   DASZYN010000042 DASZYN010000000
VERSION     DASZYN010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 10515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10515
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837437"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VHE33_01200"
     CDS             <1..394
                     /locus_tag="VHE33_01200"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HVT96091.1"
                     /translation="ATKKAPEKKKAGRGAAGGVEDYIAGIPESSRKTFDALRAAARQA
                     APKEAEEVLSYGIVGLRTDRVLVWYAAFSQHCSLFPTGSVLDQFKEELKGFKVSKGTV
                     QFPLEKPLPVALVKKLVRARVVQAGKKK"
     gene            complement(408..1127)
                     /locus_tag="VHE33_01205"
     CDS             complement(408..1127)
                     /locus_tag="VHE33_01205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT96092.1"
                     /translation="MPALDSQIALVTGAARGVGEAVSLRLASLGAHVLLVARDTKRLN
                     QVRDQIQKNQGQATVMPCDLSDTAAIARLAEQVARDHKHLDILVNNAAIGGTGQPLHE
                     VRLEDWDRLIDTNLRAPYLLIRAFAPMMIAAKSGHIVNISSLAGHNPLPNNAVYAASK
                     WGLNGLTYSVAEELRPHKVRVSVVAPGSINTHFDARHSSGKDPSKMIQPDDVASVVAT
                     IVTQTPQSFISEVLLRPTQKP"
     gene            1246..3360
                     /locus_tag="VHE33_01210"
     CDS             1246..3360
                     /locus_tag="VHE33_01210"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT96093.1"
                     /translation="MSRRQDSFAKGSASQGPATAVQRLAANAIREHQAGHLEEAKRLY
                     LEVLALDVKHAKSLFGLGLVEYQLGRYDTSARMIQRAIAVDDRTALYHSSLGAALQQL
                     QQLDSAAAAYERALALEPKNEDVHFALGNICLEQKNFAKARTHYGEALRLRPDRADTH
                     CNLGNVYLELGEAEEARACFERALALRPDFVEAETNLGNVDLKQGRLEQAAEHYQRAV
                     TLDPNRAEVHNNLGNALRGLDRIEESVAAYDRALALKPDYANAWNNRGAVLQGLGRLE
                     EAAAAHARAVELNPRDAGAWNNLGVAWREQGQLETAMQCHQRALALDANYAEAHNNLG
                     VVYRDLGRLDEAAACHERAFALKPLYAEAESNLGNVRRSQCRLVEARACYERALALKP
                     DAVDPAWNLCLVDLLEGNFERGWREYEVRKQRKRNAPRVFDAPLWRGEELNGARILLH
                     AEQGLGDTLQFLRYVPMVQARGGRVVLDVQGQLRRLAGELPGLEAVTASGENLGEFAW
                     QCPLMSLPLAFATVMDTIPAAVPYLKVPEEARRAADALPWENGRLRVGLVWSGNPKYS
                     DDRARSVPLAMMNSLFAIEGVRWFSLQLGPAAEKLAESEAPITDLRPAIGDMADTAAL
                     VERLDLVITVDTAVAHLAGALARPVWVLLPFAPDWRWLLEREDSPWYPTARLFRQPQP
                     GDWESILERVRTDLTRRAGSHE"
     gene            3353..4141
                     /locus_tag="VHE33_01215"
     CDS             3353..4141
                     /locus_tag="VHE33_01215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT96094.1"
                     /translation="MSDGRGSQGPAPVADARPVECKVCSAPSLLYGVVDFHKSCIEAQ
                     GKQLALSGRPIYYRRCQRCGFIFTTAFDTWDFDAFRKNIYNDDYVIVDPDYVEVRPAN
                     NAAMIAQSFADARGSMTILDYGGGAGLLAGRLREQGFTASTYDPFSEFKELPAEPFDL
                     ITCFEVMEHVPSPAKTVAEMVGLMKKPGAILFSTLVQPANIASIGLNWWYASPRNGHI
                     SLYTAASLATLFAPHGMKVGSFSENLHIAFGEIPKFAAHLKLAK"
     gene            4205..4783
                     /locus_tag="VHE33_01220"
     CDS             4205..4783
                     /locus_tag="VHE33_01220"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HVT96095.1"
                     /translation="MDNSKKKRCAWAESDAQMRQYHDEEWGVPEFDSRALWETLMLEG
                     FQAGLAWITVLRKREAFRKAFKGFDPKKVARLGSKDVEKMLQNPGIIRSRAKIEATIG
                     GAKAFLKMRDEGVEFSDWVWERAGGQPIQSKGPLVASTPLAETFSKELKKKGFKFVGP
                     VIVYAWMQATGIVNDHAEECFRRTVVAKMRRR"
     gene            4833..6203
                     /locus_tag="VHE33_01225"
     CDS             4833..6203
                     /locus_tag="VHE33_01225"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HVT96096.1"
                     /translation="MSASAVAYAKENAGRFLEELKALLRIPSVSTTPEHKVDVEKAAS
                     WVAADLKRIGMENVDILHSVGHPLVYADWLHAPGKPTVLCYAHYDVQPPDPLEEWVSP
                     PFEPTERNGNLYARGAVDDKGQLVSQLKALEALHAADGKFPINVRVIYEGEEEVGGER
                     IAAFVREHPERLKADFALISDTEMFAPELPTLCVGLRGMIYTELEVRGAKTDLHSGMY
                     GGAAPNPFVALAQILAGLKDRDGKILIPGFYDDVAKPSADELKAWKALPFDEEEYREK
                     EVGAIKLVGEPGYSAIERTWARPTLDVHGIVGGFTGAGAKTVIPAKATAKVSMRLVPD
                     MTPEKAFAQYKAFVEKIKPEGVTVDVRLIHQGEPIVIGTDNPYVKAATRALKKVWGKD
                     TVFIRSGGSIPIVGDFERFLKIPSVMMGFGLPDDGLHAPNEKFHIANFHRGIESIIEL
                     FDEAGR"
     gene            6431..7558
                     /locus_tag="VHE33_01230"
     CDS             6431..7558
                     /locus_tag="VHE33_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96097.1"
                     /translation="MKELVAMMTDMQQNKSDHDIAEKLMDVDLSEELTPAGIAQLAAM
                     NPGPETTEQLHILVGESSMLAPPASELPTTAAPDGAAQRAIIDKTLNYITDFMRSPKL
                     AADKTTARYQNGPDYVYSSGGGTMTLHADLGQKFSPDNPYFRNLGEHTMPVEILGGAE
                     IRPAKIKASDPSTRNGQISQGGPGPLLGVAFEDAAKGKIQFARWETVGGKALAVFSFQ
                     VPKKQSHYEVDYCCFPHTESVGSHMAQPMTSSGSGSLGSGSGISTASAVYATNTTFNP
                     FHAKVGYHGEFFIDPATGEIPRFIMQAEMRKSDFVNQEDTRIDYGPVTLEGKTIMVPQ
                     TSYVLTTVTPAGDSGQRIAERRTLFEVHYANYFQPGAGPRK"
     gene            complement(7856..8626)
                     /locus_tag="VHE33_01235"
     CDS             complement(7856..8626)
                     /locus_tag="VHE33_01235"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT96098.1"
                     /translation="MKALVLMLAAVSTLSVPALTAQSLLPPNTVTAETRQQIAKDYEM
                     KGDIARAREQYGSAVNWYLLATRYNNKDVSLYNKLGIAQMKVGDLDQARRTFGLAVKA
                     DPASVDALNNLGAAYCLQKRYKQAVRSLKQALAIDESKAVTHLNLAEAWMGQKQVDRA
                     MTEYARALELDADILNSSDKEGVFAQVQTPEQRAMVDFMIAKAYVKRGNLEGALDYLR
                     HAKEGHFRQLSSVWEDPAFAPLWKDARLAEIVKPPKAS"
     gene            8946..10514
                     /locus_tag="VHE33_01240"
     CDS             8946..10514
                     /locus_tag="VHE33_01240"
                     /inference="COORDINATES: protein motif:HMM:NF023149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HVT96099.1"
                     /translation="MAAVGHAKPMETEAWREELRTVLESRSFRQAPTLSHLLRYLCEK
                     LFAGESAQIKEYSIGVELFRRGDSFDQETDSIVRVEVNRLRKRLAEYYAGEGASHALQ
                     IVIPVGQYVPSFVSTKPETKPAPETKPETPPEPAELNEAPAWWRRRWVWAAGAGVLAL
                     AAGAVWFAEHPRHPGSQVVETPSHAQSVPEPLVGPPPGDEVRILAGSTRSYADHADKL
                     WSADTWFTGGTAVKNEARYIARTLDPSFYRASREGQFSYDIPLKRGVYEVHLYFAETE
                     YGPENTGVGGEGSRLMTVRANGQTLLTHFDVMADAGGERVADGRVFPGLSPAADGKLH
                     LQFTGENGANAMLSAIEILPGSGVGPGARMRPVRLLARQTPYYSNDSHWWSPDNYFDG
                     GQMATYTDPVSGTDDPELYETERWGNFSYAIPASPGRYTVTLQFAARHRGWNEPLAAG
                     AQETTRVAHIFNVFCNGKAILENFDLAKEAEGSDVVVRKATGLEPNAQGKLLLNFVPV
                     KGYASVTGIEVVPE"
BASE COUNT         2073 a         2871 c         3685 g         1886 t
ORIGIN      
        1 cgcgacgaag aaagctcccg aaaaaaagaa agcaggccgc ggagctgccg gcggcgtcga
       61 ggactacata gccggaattc cggagagcag ccggaagact ttcgatgctc ttcgtgcggc
      121 ggcgcgccag gcagcgccaa aggaagctga ggaagtcctg agttacggga ttgtggggct
      181 gcgaacggat cgggtgctgg tgtggtatgc ggcgttctcg cagcactgca gcttgtttcc
      241 gacgggttcg gtgctggatc aattcaaaga ggagctgaag ggattcaagg tgtcgaaggg
      301 gacagtgcag tttcccctgg agaagccgct gccggtcgcg ctggtgaaaa agctggtgcg
      361 ggcacgggtg gtgcaggcgg gaaagaagaa ataaagctca ggttcgctca gggtttttgt
      421 gtaggtcgaa gcagtacttc gctgataaag gactgcggcg tctgcgtgac gatggtcgcc
      481 accacggacg cgacatcgtc gggctgaatc atcttcgagg gatccttgcc gctgctgtgg
      541 cgggcgtcga agtgcgtatt aatggagccg ggcgcgacaa cgctgacgcg gactttgtgc
      601 ggacgcagct cttcggcgac cgaataggtg agcccgttga gtccccactt cgaggctgca
      661 tagaccgcat tgttgggcag cgggttgtga ccggcgagcg aagaaatgtt gacgatatgg
      721 cctgacttcg cagcgatcat catgggcgcg aaggcgcgga tcagcaggta gggcgcgcgc
      781 agattcgtat cgatgaggcg gtcccaatcc tcgaggcgga cctcatggag cggctggccg
      841 gtgcctccaa tggccgcgtt gttgacgagg atgtcgagat gtttgtgatc gcgtgcgacc
      901 tgttcggcga ggcgggcgat cgctgcggta tcgctgaggt cgcagggcat gacggtcgcc
      961 tggccctggt tcttctggat ctgatcgcgg acctggttga ggcgcttcgt gtcgcgcgcg
     1021 accagcagca catgggcgcc gagagaggcg aggcgaagcg agacagcttc tccaacgccg
     1081 cgagcggcgc cggtgacaag ggcgatttga gaatcgagag cgggcatagc ttctattttc
     1141 gatgcgcggc gggcctgagg agaagggcag gaatcccggc gcggccccgt atacttttgt
     1201 gtcaacgatg cgaatgaagg tgaatacaca cacagggctc ggaagatgag ccggcggcag
     1261 gattcgtttg cgaagggctc tgcttcccag gggccggcga cggcggtgca gcggctggcc
     1321 gcgaatgcga ttcgcgagca ccaggcgggc catctggaag aggcgaaacg gctctacctc
     1381 gaagtgctgg cgctcgacgt aaagcatgca aagagcctct tcgggctggg gctggtggag
     1441 tatcagctcg gtcgttacga tacctcggct cgcatgatcc agcgggcgat cgcggtggac
     1501 gatcggacgg cgctgtacca ctcaagcctg ggcgcggcgc tgcaacagct gcagcagctg
     1561 gacagcgcgg cggcagcgta tgagcgggcg ctcgcgctgg agccgaagaa cgaggacgtc
     1621 cacttcgcgc tgggcaatat ttgtctggag caaaagaatt tcgcaaaagc ccgcacgcat
     1681 tatggtgaag cgcttcgact gcggcccgat cgcgccgaca cgcactgcaa cctcggcaat
     1741 gtgtatctgg aactgggcga agcggaggag gcacgcgcct gctttgagcg cgccctggcg
     1801 ctccggcctg attttgttga ggcggaaaca aacctgggca atgtggatct gaagcagggg
     1861 cggctggagc aggcagcgga gcactaccag cgagctgtga cccttgatcc gaatcgcgcc
     1921 gaagtgcata acaacctcgg caatgcgctg cgcggactgg atcgtatcga agaatctgtg
     1981 gcggcttacg acagggcgct tgcgctgaag ccggattacg ccaatgcctg gaacaatcgt
     2041 ggtgcggttt tgcaggggtt gggccgtctg gaagaggcgg ccgcagcgca cgcgcgcgcg
     2101 gtggagctga atcccaggga tgcgggcgcg tggaacaacc tgggcgtggc atggcgcgag
     2161 cagggccagt tggagacggc gatgcagtgt catcagcgtg cgctcgcgct cgacgcgaac
     2221 tatgccgagg cacacaacaa cctgggcgtg gtatatcgcg atctggggcg gctggacgag
     2281 gctgcagcgt gccacgagcg cgccttcgca ctcaagcccc tctatgccga ggcagaaagc
     2341 aatctgggca atgtgcggcg cagccagtgc cgcctggtcg aagcgcgcgc atgctatgaa
     2401 cgggccctgg cgttgaagcc ggacgcggtg gatccggcgt ggaatctctg cctggtggat
     2461 ctgctggagg gcaatttcga gcgcggctgg cgcgagtatg aggtccggaa acaaagaaag
     2521 cgaaatgcgc cgcgggtttt cgatgcgccg ttgtggcggg gagaagaatt gaacggcgcg
     2581 cggatcctgc tgcatgccga acagggttta ggggacacgc tgcagttcct gcgttacgta
     2641 ccaatggtgc aggcgcgggg tgggcgcgtt gtgctggatg tccaggggca gctgcgcagg
     2701 cttgccggcg aactgccggg actcgaagcg gtgacggcaa gcggtgaaaa tttgggtgaa
     2761 tttgcctggc aatgcccgct gatgagcctg cctctggcat ttgcaaccgt gatggataca
     2821 attccggcgg cggtgcccta tctgaaagtg ccggaggagg cgcgacgagc agccgatgcg
     2881 ttgccctggg agaacggccg gctgcgggtg gggctggtgt ggagcggcaa cccgaagtac
     2941 agcgacgatc gggcgcgctc ggtgccgctg gcgatgatga attcgctgtt tgcgatcgaa
     3001 ggagtgcggt ggttctcgct gcaactgggg ccggccgccg agaaactggc cgaaagcgaa
     3061 gctccgatca ccgacctgcg tccggcgatc ggcgacatgg cggacacagc ggcgctggtg
     3121 gagcgcctgg atctggtgat cacggtcgac acagccgtcg cgcatctggc cggagcgctg
     3181 gcgcgacccg tgtgggtgct gctgccgttt gctccggact ggcgctggct gctggagcgc
     3241 gaagacagcc cctggtatcc gacggcgcgg ctgtttcgtc aaccacagcc cggcgattgg
     3301 gagagcatcc tggagcgggt tcgcacagac ctgacaagga gagcaggctc gcatgagtga
     3361 cgggcgcgga tcacaggggc cggcgccggt ggccgatgcg cggccggtgg agtgcaaggt
     3421 gtgcagcgcc ccaagcctgc tctatggcgt cgtggatttt cacaaaagct gcatcgaggc
     3481 gcagggcaag caactggcgt tgtcaggacg cccgatctac taccggcgct gccagcgatg
     3541 cggtttcatc tttacgacgg cattcgacac atgggacttc gacgcgttcc ggaaaaacat
     3601 ttacaacgac gactacgtga tcgtcgatcc ggactatgtg gaggtgcggc cggccaacaa
     3661 tgcggccatg atcgcgcaaa gcttcgcgga tgcgaggggg tcgatgacga ttctcgacta
     3721 cggcggcggc gcgggactgc tcgccggccg gctgcgcgag cagggattca cggccagcac
     3781 ctacgatcca ttttcggagt ttaaggaact gccggccgaa ccgttcgatc tgatcacatg
     3841 ctttgaggtg atggagcacg tgccctctcc tgcgaaaacg gtggcagaga tggtcggcct
     3901 gatgaagaag ccgggggcga ttcttttctc gacgctggtg cagccggcca atattgcttc
     3961 gatcggcttg aactggtggt atgcgagtcc acggaacggc cacatctcgc tctacacggc
     4021 cgcgtcgctg gcgacgctgt tcgcgccgca tgggatgaag gtggggtcat tctcagagaa
     4081 tctgcatatt gcgtttgggg agattccaaa gtttgccgcg catttgaaat tggcgaagtg
     4141 aagcggagga gttagggagt agggagtagc gagcaggcag agcaaaagac agaggtcggc
     4201 ggggatggat aacagcaaga agaagcgttg cgcgtgggcg gaaagcgatg cgcagatgcg
     4261 gcagtatcac gatgaagaat ggggagtgcc ggagttcgac agccgcgcgt tgtgggagac
     4321 gctcatgctc gaaggattcc aggccgggct cgcgtggatt acggtcctgc gcaagcggga
     4381 ggcgttccgg aaggcgttca aggggttcga tccaaagaag gtggcgcggc ttggctcgaa
     4441 ggatgtggag aagatgctgc agaatccggg catcatccga tcccgcgcga agatcgaggc
     4501 cacgatcggc ggggcgaagg cctttttgaa gatgcgggac gagggcgtgg agttttcgga
     4561 ctgggtgtgg gagagggcgg gcggacagcc gatccagagt aagggcccgc tggtcgcgag
     4621 cacgccgctg gcggagacgt tttcaaaaga gctgaagaag aaaggcttca agtttgtggg
     4681 gccggtgatt gtgtatgcct ggatgcaggc aacaggaatc gtgaacgatc acgcggaaga
     4741 gtgtttccgg cggactgtgg tcgcgaaaat gagacgccgt tagggcgaac gcagcgagac
     4801 gtatactcga tggtttgctg aaaggtgaag agatgagtgc cagcgcagtt gcgtacgcca
     4861 aagagaatgc aggcaggttt ctggaggagc tgaaagcgct gctgcggatt ccgtcggtgt
     4921 cgaccacgcc ggaacataag gtcgatgtgg agaaggcggc gtcctgggtc gcagcggacc
     4981 tgaaacgcat cggcatggag aacgtcgata tcctccacag cgttgggcat ccgctcgtct
     5041 atgccgactg gctgcatgcg cctggaaagc cgaccgtcct ctgctacgcg cactatgacg
     5101 ttcagccgcc cgatccgctc gaggaatggg tttcaccgcc gtttgagccg accgagcgca
     5161 acggcaatct ctacgcgcgc ggcgcggtcg acgacaaagg gcagctggtc tcgcagctga
     5221 aggcgctcga agcattgcat gcagcagacg ggaagtttcc gatcaacgtg agggtgatct
     5281 acgagggtga ggaagaggtc ggcggcgaga ggatcgcagc gttcgtgcgc gagcacccgg
     5341 agcggctgaa ggcggacttc gcgctgatct ccgacactga gatgtttgcg ccggagctgc
     5401 ccacgctgtg cgtggggttg cgcggcatga tctacaccga gctggaggtg cgcggggcga
     5461 agaccgatct gcattccggg atgtatggcg gcgcggcgcc gaatccgttt gttgcgctgg
     5521 cgcaaattct cgcaggcctc aaggatcgcg acgggaagat cctgatcccg ggcttctacg
     5581 acgatgttgc gaagccgagc gccgatgagc tgaaggcatg gaaagcgctg ccgtttgatg
     5641 aagaagaata tcgcgagaag gaagtgggcg cgatcaagct ggtgggcgag ccgggctatt
     5701 cggccatcga gcggacctgg gctcgaccca cgctcgacgt gcacggcatc gtcggcggat
     5761 tcacgggcgc gggcgcgaag accgtgattc ccgcgaaggc gaccgcgaaa gtctccatgc
     5821 gcctggtgcc cgatatgacg ccggagaagg ccttcgccca gtacaaggcg ttcgtcgaga
     5881 agatcaaacc ggagggcgtt acggtggacg ttcggctgat tcatcagggc gagccgatcg
     5941 tgatcggcac ggacaatccc tatgtgaagg cagcgacccg ggcgctcaaa aaggtgtggg
     6001 gaaaggacac ggtgttcatc cgctcggggg ggtcgattcc gatcgtcggc gacttcgagc
     6061 gcttcctgaa gatcccgtcg gtgatgatgg gcttcggatt gcccgacgat gggctgcatg
     6121 cgcccaacga gaagttccac atcgcgaact tccatcgcgg cattgaaagc atcatcgagt
     6181 tattcgatga ggccggtcgt tagcggccag gcagggagcg agccagacag gccagaaacg
     6241 aatgcttgca ttaaccattt agttgctatc gtgccatgcc cggaagtgga ataatgcagt
     6301 cgcctgcacg gtcaggctga ttctgtcgca tgcgcgtcga atccgaggcc ctatgaaaat
     6361 ccggttcgca cttctggctg cactgattgc agctgtgccc ttttctctgt tcgctgccag
     6421 gacgatgtcc gtgaaggaac tggtcgcgat gatgacagac atgcaacaaa acaagtccga
     6481 ccacgatatt gcggaaaagc tgatggacgt cgatctgtcc gaggagctga cgccggcggg
     6541 gatagcgcaa ctggcggcca tgaatccggg accggagact accgagcaac tccacattct
     6601 ggtgggagag agttcgatgc tggcgccgcc ggcctcggaa ctgccaacga cggctgctcc
     6661 cgatggggcc gcgcaaagag cgatcatcga caagacgctg aattacatca ccgacttcat
     6721 gcgttcgccc aagctggcgg cggacaagac gacggcgcgc tatcagaacg ggccggacta
     6781 tgtgtactcc tccggcggcg ggacgatgac tctgcacgcc gacctgggcc agaagttctc
     6841 tcccgacaat ccttatttca ggaacctggg agagcacacc atgccggtgg agatcctggg
     6901 cggcgcggaa attcgtccgg caaagatcaa ggccagcgat ccgtcaacgc gcaatggcca
     6961 gatctcgcag ggcgggccgg ggccactgct gggggtggcg tttgaagatg cggcaaaggg
     7021 aaagatccag ttcgcgcgct gggagacggt ggggggtaag gcgctggcgg ttttctcctt
     7081 ccaggtgccg aagaagcagt ctcactacga ggtggattac tgctgctttc cgcacaccga
     7141 aagcgtgggg agccatatgg cccagcccat gacatcttca ggatccggtt ccctgggatc
     7201 gggatcgggc atttctactg cgagcgctgt ctatgccacg aatacgacct tcaatccgtt
     7261 ccacgcgaaa gtcggttatc acggggagtt tttcatcgat ccggcgaccg gggagatacc
     7321 gcggttcatt atgcaggcgg agatgcggaa gtccgatttc gtgaaccagg aagatacgcg
     7381 tatcgattac ggtccggtaa cgctggaggg caagacaatc atggtgcctc agaccagcta
     7441 tgtgctgacc acggtgaccc ctgcgggaga ttccgggcag cgcatcgccg agaggcgcac
     7501 gttgtttgag gtgcactacg cgaattattt tcagccggga gccgggccac ggaaataagc
     7561 cggctccttc gcctgagtgg gctcagtttc acttcgaaaa agaagggaag cggtccagag
     7621 gagttcgccc cagcgagaga cagatgaacc tctgggggaa aggaccgctt cccggcgaag
     7681 tgaggcaact acacttgaga acttcgcgca tccggcaagc agccggacgc ggctgaagag
     7741 tacacccggc gagctgcctg cattcctcat cccacgattg agttgcagga cagctcgccg
     7801 ggatcggcgc gcgggaactc gccggttggg ctcgttccct ggcgccaaac ggagctcagg
     7861 atgctttggg cggcttcacg atttcggcca gccgcgcatc cttccagagt ggcgcaaacg
     7921 cgggatcctc ccacacgctg gagagctgac ggaagtgccc ttctttggcg tggcgcagat
     7981 agtccagcgc gccttcgagg ttgccgcgct tgacgtaggc tttggcgatc atgaagtcga
     8041 ccatcgcgcg ctgctcgggc gtttgaacct gcgcaaacac gccttccttg tcggagctgt
     8101 tcaggatgtc ggcatcgagt tcgagagcgc gggcatactc ggtcatggcg cggtcgacct
     8161 gcttttgccc catccaggcc tcggcgaggt tcaggtgggt cacggctttc gattcatcaa
     8221 tggccagcgc ctgtttcagc gagcgaaccg cctgcttgta cctcttctgc aggcaatacg
     8281 ccgctcccag gttgttgagg gcgtccacgc tggcgggatc ggccttgacc gccaggccaa
     8341 acgtccgccg ggcctggtcg agatccccca ccttcatctg ggcgatgccc agcttgttgt
     8401 agagagaaac atccttattg ttgtagcggg tggcgagcag gtaccagttg actgccgagc
     8461 cgtactgttc gcgggcgcgg gcaatgtcac ccttcatttc gtaatctttg gcaatttgct
     8521 gccgagtctc cgccgtaacg gtattgggcg gcagcaggga ctgcgcggtg agggccggga
     8581 cgctcagggt ggaaaccgcg gccagcatca ggacgagggc tttcatacga ctacctcccg
     8641 cggaaggctg tgtgggcagt tgggggagct ttctcttccg catcgaggct cagtacatac
     8701 ctctccaccg gcccggaaca acgctagagc ggctaccgtg gggcaactcg ggggctacac
     8761 gagtggctat ggtattcgat cgcacattca acgagactta gcgggagtgg tcagtgggcg
     8821 gccccggggg gttaaatcca gttaaatcgg acgtacccgg cttcctgtga cggttaagcg
     8881 gcaccgggag ccggttacaa gcgtataaag aaggcgtcga gagcgaagga cgagcgagta
     8941 cgcgcatggc ggcagtcgga cacgcaaagc caatggaaac ggaggcgtgg cgcgaggagc
     9001 tgcgcaccgt gctggagtcg cgctcttttc gccaggcgcc gaccctctcc cacctgctgc
     9061 gttatctctg cgaaaagctc tttgccggcg agagcgccca gatcaaggaa tattcgatcg
     9121 gggtggaact gttccgccgc ggcgactcct tcgaccagga gaccgactcc atcgtgcggg
     9181 tggaagtgaa ccgcctgcgc aagcggctgg ccgagtacta cgccggggag ggcgcgtcgc
     9241 acgcgctgca gatcgtgatc cccgtcggcc agtacgtgcc gagctttgtg agcaccaagc
     9301 cggagacgaa accagccccg gagacgaagc cggagactcc acccgagccg gccgagctga
     9361 acgaggcgcc ggcatggtgg cgtcggcgat gggtgtgggc cgctggagcc ggggtcctgg
     9421 ctctcgcagc gggcgcagtc tggttcgcgg aacatccgcg gcatccgggg tcgcaggtcg
     9481 tagagacgcc atcccacgcc caaagcgtgc ccgagcccct ggtgggtccg ccgcctggcg
     9541 atgaggtgcg catcctggca gggtcgacac gcagctacgc cgaccatgcg gacaagctat
     9601 ggagcgccga tacctggttt acgggcggca cggcggtcaa gaatgaagcg cgctacatcg
     9661 cgcgcaccct cgatccgagc ttttatcgag ccagccgcga agggcagttc agttacgaca
     9721 ttccgctgaa gcgcggcgtg tatgaggtgc acctgtactt cgcggagacg gagtacggac
     9781 cggagaatac cggggtcggc ggcgaaggca gccgcctgat gacggtccgc gccaatggtc
     9841 agacgttgct gacgcacttt gacgtgatgg ccgatgcggg cggggagcgg gtcgccgatg
     9901 ggcgggtctt tccgggactg tctccggcgg ccgacgggaa actccacctg cagttcaccg
     9961 gcgaaaacgg cgccaacgct atgttgtcgg cgatcgagat tctgcccggg tcgggagtcg
    10021 ggccgggggc gcgcatgcgg cccgtgcggc ttctggcgcg ccagacgcct tactactcca
    10081 acgacagcca ctggtggagt ccggacaatt acttcgacgg tgggcagatg gcgacctaca
    10141 ccgatccggt gagcgggacc gacgatccgg aactgtatga gaccgaacgc tgggggaatt
    10201 tctcctatgc cattcctgcg tcccctgggc ggtacacggt gacattgcaa ttcgcggcgc
    10261 ggcatcgcgg atggaacgag cctctggccg ccggcgcgca ggagacgacg cgcgtggcgc
    10321 acatcttcaa cgtcttctgc aacggcaagg cgatcctcga gaactttgat ctggcgaaag
    10381 aggccgaggg ctctgacgtt gtggtgcgca aggcgacggg cctcgaaccg aacgcgcagg
    10441 ggaaactgct gctgaacttc gtgccggtga agggctatgc gtcggtgacg gggattgaag
    10501 tggtgccgga gtagg
//
LOCUS       DASZYN010000043         3082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_2063, whole genome shotgun sequence.
ACCESSION   DASZYN010000043 DASZYN010000000
VERSION     DASZYN010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2063"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1562
                     /locus_tag="VHE33_01245"
     CDS             <1..1562
                     /locus_tag="VHE33_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT96100.1"
                     /translation="GEFVTTLGSYYQTDSQLSLGNHSERFAWFAAVNGNRSNYGLMPP
                     IEKPVHDAENGYGGFTSLIFNKNPHDQLRFVGQMRTDYYQIPYDPDPSSWENQLYDSS
                     GLRDGQHETDGLTTFSWIHTLSAKSLFEVSPFYHYNDANYEPSPNDQPNATHAKQTGQ
                     YAGAQAYFSTTVARNAFKAGLYGYGQHENDLFGVTFNDGSAPNFSETSVVNGGVQESF
                     IEDNYRPTSWLTLIGGMRQAHFQGAVSENAIYPRLGAAVQIPKLGWVLRGFYGHFYQP
                     PPLTSISGPALAFAQASDTNFVPLPGERDEEHQFGIQIPFRGWLLDADTFQTRVNNFL
                     DHSNIGESSIFVPVTIDGALIQGWELTLRTPRLWRYGTGHLAYSNQIAKQRGELTGGL
                     ICSEGSPDCDAEPDYSPLDHDQRNTLNVGFDGSLPHRAWASFNIYYGSGFANGITDPP
                     SPYTGDYLPAHTMWDLAVGKAIAENWSLAVNAVNVANTRVLLDNSLTFGGFHENDPRQ
                     IYGQVRYRFHF"
     gene            1572..2459
                     /locus_tag="VHE33_01250"
     CDS             1572..2459
                     /locus_tag="VHE33_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96101.1"
                     /translation="MALAAPLPTVAARPRSRLWGVPIYWHLLSLDAPTVAVLWAWSFA
                     SAAHLRIPFVSIAVLGVGTWLIYIADRLLDVRSPSHPALRERHFFHERHRRVLFLVSP
                     VVGALLLGLICAMPPAARRDDTILFAISMLYFGGVHLPALRVRRWFPREVAVGILFAL
                     ATAVPAWSAPDSKPQLAWFVLLFAGLCTLNCIAIETWERSSNTPRSMTVSAMAICAAV
                     ASIALLTMRGHQLPGEFAICASAFTSAALLFALDGVHRRWFSRSSWPEDRRHFLLALR
                     VAADAALLTPLFFLSLWHL"
     gene            2447..>3082
                     /locus_tag="VHE33_01255"
     CDS             2447..>3082
                     /locus_tag="VHE33_01255"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT96102.1"
                     /translation="MASVKQIPQLRVNKARPFAATDLDLLRNPPARFDRVARFYRAME
                     YLSFGPLLERCRFHHIPRLVDARRALVLGDGDGRFVARLLSSAPHLSADALDASSAML
                     HLLRERVARQGALHRLTTTCADARIFGAPCAGYDLVVTHFFLDCLTEQETDRLIARLR
                     PHLVPGARWLVSEFEVPRSGALQRSLAQSVVSGLYAAFRLLTGLQVRQIPAW"
BASE COUNT          499 a         1043 c          891 g          649 t
ORIGIN      
        1 agggtgaatt tgtaacaacg ctgggcagtt attaccagac cgacagccag ctgagcctcg
       61 gcaaccacag cgagcgcttt gcgtggttcg ccgccgtaaa cggcaatcgc agcaactatg
      121 gcctgatgcc gcccatcgag aaaccggtcc acgacgcaga gaacggctat ggcggcttca
      181 cgtcgctcat cttcaacaag aatccgcacg accagctgcg ttttgtggga cagatgcgca
      241 ccgactacta ccagattccg tatgatccgg atccgagcag ctgggagaac cagctgtacg
      301 actcctcggg tttgcgcgac gggcagcacg aaaccgacgg tctgacgacc ttctcgtgga
      361 ttcacaccct gagcgcgaaa tcgcttttcg aggtttcgcc gttctatcac tacaacgatg
      421 cgaactacga gccgagtccg aacgatcagc ccaacgccac ccatgcgaaa cagacgggcc
      481 agtacgcggg cgcgcaggcg tatttctcca cgaccgtcgc gcgcaatgcg ttcaaggccg
      541 gactctatgg ctacggccag catgagaacg atctttttgg cgtgactttt aacgatggca
      601 gtgcgccgaa tttttccgag acctccgttg tcaacggcgg cgtccaggaa tccttcatcg
      661 aggacaacta ccgcccgact tcctggctga cgctcatcgg cggtatgcgc caggcgcatt
      721 tccagggcgc ggtctccgaa aacgcgatct acccgcgtct gggcgccgcg gtgcagattc
      781 ccaaactcgg ctgggtgctg cgcggtttct acgggcactt ctatcagccg cccccgctca
      841 ccagcatctc cggtcccgcg ctggcgttcg cgcaggcgag cgacaccaac tttgtcccgt
      901 tgccagggga gcgggacgaa gagcatcagt tcggcatcca gattcccttt cgtggctggc
      961 tcctcgatgc ggataccttc cagacgcggg tcaacaattt tctcgaccac agcaacatcg
     1021 gcgaatcgag catctttgtt ccggtgacga ttgacggcgc gttgatccag ggctgggagc
     1081 tgaccctgcg gacgccgcgc ctgtggcgct atggcacggg acatctcgcc tactccaacc
     1141 agatcgcgaa gcagcgtggc gaactgaccg gcgggctcat ctgctcggag ggctcgccgg
     1201 actgcgatgc tgagccggac tactcgccgc tcgatcacga ccagcgcaac acgctcaacg
     1261 tgggattcga cggcagcctg ccgcatcggg cctgggcctc gttcaacatt tattacggat
     1321 cggggttcgc gaacggcatt accgatccgc cctcacccta taccggcgac tacctgcccg
     1381 cgcacaccat gtgggatttg gccgtgggca aagccatcgc tgagaactgg tcgcttgcgg
     1441 tgaatgccgt gaacgtcgcc aacacgcggg tgctgctcga caacagcctt acgttcggcg
     1501 gcttccacga aaatgatccg cgccagattt acgggcaggt tcggtaccgt tttcactttt
     1561 agaattgaag agtggctctt gctgctccgc tccccacagt cgccgcacgg cctcgctcac
     1621 gactgtgggg agttcccatc tactggcacc tgctcagcct ggatgcgccc acggtggccg
     1681 tcctctgggc ctggagcttt gcctccgccg cgcacctgcg catccctttt gtttcgatcg
     1741 ccgttctcgg cgtcggcaca tggctgatct acatcgcgga ccggctgctc gacgtccgct
     1801 caccctcgca tcctgcgctg cgcgagcggc acttctttca cgagcggcat cgtcgcgtgc
     1861 tgttcctggt ctcgccggtc gtgggagcgc tgctgctggg cctgatctgc gccatgcccc
     1921 cggccgcacg acgcgacgat acgattctgt tcgccatttc catgctgtac tttggcggcg
     1981 tgcacctgcc ggctcttcgc gtgcgccgct ggtttccgcg cgaggttgcc gtcggcattt
     2041 tgttcgcttt ggcgaccgcg gtgcccgcct ggtccgcccc ggattcgaag cctcagctcg
     2101 cctggttcgt acttctcttt gccgggctgt gtacgctgaa ctgcatcgcc atcgaaacct
     2161 gggagcgatc ctcaaacacg ccccgctcca tgaccgtttc cgccatggcg atttgcgccg
     2221 cggtcgcgtc aatcgcgctc ctgacgatgc gcggacatca actccctggc gaattcgcga
     2281 tctgcgcatc cgcgttcact tcggcagccc ttctgttcgc gctcgacggc gttcaccgtc
     2341 gctggttcag ccgaagttcg tggccggaag accgccggca ttttctgctg gcccttcgcg
     2401 tagctgccga tgctgcgctg ctcacacccc tgttcttcct ttctctatgg catctgtaaa
     2461 acaaattccc caactgcgcg taaacaaggc gcgcccgttc gcggcgacgg acctcgatct
     2521 gctccggaat ccgcctgcgc gcttcgaccg cgtcgcccgc ttctaccgcg cgatggagta
     2581 tctctccttc ggcccgctgc tggagcgctg ccggttccac cacatcccga ggctggtgga
     2641 tgcccgccgt gctctggtcc ttggtgatgg cgacggccga ttcgtggcgc gactgctctc
     2701 ctccgcaccg catctctcgg cggatgccct ggacgcctca tcggcgatgc ttcacctgct
     2761 tcgcgaacgc gtcgccagac agggcgctct gcaccgcctg acgaccacct gcgccgatgc
     2821 gcgcatcttc ggggctcctt gcgcgggtta cgacctcgtg gtcacgcact tcttcctcga
     2881 ttgcctgact gagcaggaga ccgatcgcct catcgcgcgt ttgcggccgc atctggtgcc
     2941 tggagcgcga tggctggtct ctgaatttga ggtgccgcgg tccggagcgt tgcaaagaag
     3001 ccttgcccaa agcgtggtct cgggcctcta cgctgcattt cgcctgctca ccggcctcca
     3061 ggtgcgtcag attccagcgt gg
//
LOCUS       DASZYN010000044         7938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_195034, whole genome shotgun sequence.
ACCESSION   DASZYN010000044 DASZYN010000000
VERSION     DASZYN010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195034"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VHE33_01260"
     CDS             <1..294
                     /locus_tag="VHE33_01260"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HVT96103.1"
                     /translation="WPILLSFPLPYIANTAGWMTAEIGRQPWLVYGLIRTSQGYSTHV
                     SAGNALFTLLGFLGMYSILSILWIVLVYRFIHTGPAGVAVTLPEPEPASLTTV"
     gene            314..1357
                     /gene="cydB"
                     /locus_tag="VHE33_01265"
     CDS             314..1357
                     /gene="cydB"
                     /locus_tag="VHE33_01265"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HVT96104.1"
                     /translation="MGFVWFWIVAVMIAAYVVLDGFDLGVGILSPFLGRSEDDRRTLF
                     RSIGPVWDGNEVWLLAGGGTLYFAFPLLYASSFSGFYLPLMIVLWLLIMRGASIELRM
                     HIDHDVWRSFFDGLFFIASALLAIFFGAALANVIRGVPLGADNYFFLPLWTNWRVGPA
                     PGILDWYTVIGGVVALVALAVHGVLWIVLKAPGELETRARRAVVPLWTALLVITLISL
                     PATIAVRPSALENYKAYPVLFLIPALVIATLIGILTFTKQKKPLAAFLSSCLYLVFML
                     VGAAAGLYPTLLPSSSDPSRNITIASAIAGPHSLHVGLIWWSLGLILALAYFITSYWL
                     FRGKVSAEAGYGH"
     gene            1384..4128
                     /locus_tag="VHE33_01270"
     CDS             1384..4128
                     /locus_tag="VHE33_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HVT96105.1"
                     /translation="MASATVAPDTPLIIDPAHDLVSIQHSPLASIFTPKSVALIGATE
                     RVGSVGRSVMMNLTSSSFGGPVWPVNPKSPTILGIPAYKSIKELPGVPDLVVVTTPAA
                     TVPGIIQEAVDLGVPSAVVISAGFKEVGESGKALERQIAQIIRGRMRLIGPNCLGVMN
                     PVGGLNATFAKAMVPAGRLAFISQSGALLTAILDWCIREHVGLSGCVSAGSMLDITFG
                     ELIDHYGKDPNTDSIVLYMESIGDARSFLSAAREVALNKPILVIKAGRTAAAAKAAAS
                     HTGSLTGSDDVLDAAFQRAGVLRVQSISEIFNISEVLAKQPRPKGPRLTILTNAGGPG
                     VLATDALISAGGELTEISPETMTTYDNFLPSAWSHNNPVDILGDAEPERYAKALEIAS
                     QDKNTDGMLVILTPQGMTNPTEVAEHLIPYARSTGKPVIASWMGGDYVARANEVLAKA
                     GIPAFAYPDAAAEAFVATWKHTSNLRDLYETPTTRSGSVLDTKRDLAGSIVDQVRARR
                     RTILTEYESKQLLAAYGIPTVRTELATTPEEAAQKALEIGYPVVLKLHSETITHKTDV
                     GGVKLNLRSAGEVVTAFSDIQKSVTEKVGAEHFLGVTVQRFAKLEGYEIILGSSIDPQ
                     LGPVLLFGTGGQLVEVYKDRALALPPLTATLARHQMEQTKIFKALKGVRGRKPVDLDA
                     LDALVVRFSELVVEQRWIREIDINPLLASPDGLIALDARVVLHEPGTTEDQLPRPAIR
                     PYPVQYIGKWESRSGADVLVRPIRPEDEPLIVDFHDRLSERTVVQRYFTSLALSARTA
                     HERLTRICFIDYDRELALVAEVNDPKKGKLIAGVSRMIRQNHGQNATLAVVIADDFQG
                     QGLGGELMRRTLAAARAEGIEQVQVYFLPENNRMRKLSQKLGFTLTEENGLMRGELTV
                     "
     gene            complement(4221..5648)
                     /locus_tag="VHE33_01275"
     CDS             complement(4221..5648)
                     /locus_tag="VHE33_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVT96106.1"
                     /translation="MRRYSLRRRLIAAVLALECGLVLGLSSATLLYLWREQIRNFDLM
                     VRGRADSLLGQVHDAEDPNDSVAINLAALDLRGNDLWMARDEGGRTLAQSTSWSSAAL
                     HDFAAKDVPHDFRVRGRTFRGLVLHGVRQIDAEDGSPGIARPVTIFYAAPLEPVYGSI
                     RRAAAFLVIASLLLLLITAGVLAQVLRRGLAPLEALSRAAGEMTPKRPRFEAPRSARS
                     TVELTVLARALESATERLEEAFVQQRMFVNDAAHELKTAVTIIKSSLQLLASRPRSAR
                     EYARGLETCMADCARMEELVQRMLQLARFEREPASGDVECNVAEVARDVAVHMERLAE
                     IREVKIAVHAPEPAPTRLERDACASLLANLMLNAVQHTPEGGSVTAAIAAGTSVVIEV
                     RDTGRGIAPEDLPHLFERFWRGDHSRARSTGGAGLGLSICKAIVDSCGGDIAVNSRLG
                     EGTCVTVTLPLVASAEAVRELSIVS"
     gene            complement(5645..6349)
                     /locus_tag="VHE33_01280"
     CDS             complement(5645..6349)
                     /locus_tag="VHE33_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT96107.1"
                     /translation="MLPSMRALVIEDEARVAGNIAEALRESAGLAVDVARTGLDGAEL
                     GRQANYDLIVLDLMLPGMDGLAVLKTMRAKGQTAPVLVLTAVGEKTSIVKLLNAGADD
                     YLPKPFDLGEMLARAKALIRRGKGVSHPKIELADLVIDTLERSVTVGGKPVELSPTEY
                     RALEYLSHRPRSIISKQVLLEHLYDYNWEHHSNVIEAHISNLRRKLNRDGEGHWIETL
                     RGRGYRLMTGASGGAA"
     gene            6451..7626
                     /locus_tag="VHE33_01285"
     CDS             6451..7626
                     /locus_tag="VHE33_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HVT96108.1"
                     /translation="MRRIYMDANATTPLLPEVFEAMRPFFIESFGNASSIHHFGQRAR
                     AAVEQAREQVAALLHCRPAEIVFTSCGTESDNTAIFGILKPGDHLITTSIEHHAVLHA
                     AEKAEARGVAVTVLSVGPSGVIDPAEVRAALKPNTKLISIMMANNETGVVQPVEAIGK
                     IAAEAGVTFHTDAVQAAGKIPIDVAAIGCHLLSISGHKFHGPQGIGVLYVRRGTHFEP
                     LVFGGSHERQRRAGTENVPGIVGLGKAAEIALAAITDGTDERVGHLRDRLEQGLLARV
                     DECGINGADRSRVPNTTNIYFDHLEGEALVIALDLKGLACSGGSACSSGSPEPSHVLN
                     SMGISQARARASLRFSLSRLTTDEDVDAALDLVPGAVAHLRELSPVYKKHLTEAAAQ"
     gene            7663..>7938
                     /locus_tag="VHE33_01290"
     CDS             7663..>7938
                     /locus_tag="VHE33_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96109.1"
                     /translation="MKRRSFFRMAAGSAGMLWTSGLERSLQALAQGPASSAVRRVLVM
                     FKCHFDAGFIDTQTAVVHKYFSQYFPQAIRVAQQSREPGQHRYVWTTG"
BASE COUNT         1446 a         2773 c         2192 g         1527 t
ORIGIN      
        1 tggcccatcc tgctcagctt cccgctgcct tacatcgcta atactgccgg ctggatgacc
       61 gctgaaatcg gccgtcagcc ctggctggtg tacggcctga tccgcacttc gcagggttat
      121 tcgacccatg tttcagccgg caatgcgctg ttcactctgc tcggttttct tggcatgtat
      181 tcgatccttt cgattctctg gatcgtcctc gtctatcgct tcatccacac aggcccggca
      241 ggcgtcgctg tcactctgcc cgaacctgaa cctgcatcgc tgacgacggt ctagaaaaga
      301 gaggagaacg aaaatgggat tcgtctggtt ctggattgtc gcagtcatga tcgccgccta
      361 cgtcgtactc gacggcttcg atctgggcgt gggtattctt tcgccattcc tcggccgcag
      421 cgaagacgat cggcgcacgc tgttccgctc cattggcccg gtgtgggatg gcaacgaggt
      481 ttggctgctg gctggcggtg gcacgctcta ttttgctttt cctctgctct acgcttcctc
      541 gttcagcggt ttctatctgc ccctgatgat cgtgctgtgg ctgctcatta tgcgcggcgc
      601 ctcaatcgag ctgcgcatgc acatcgatca tgatgtctgg cggagttttt tcgacggcct
      661 cttcttcatc gccagcgcgc tgctcgcgat cttctttggc gcggccctgg ccaacgtcat
      721 ccgcggcgtg cctctcggcg ctgacaacta cttcttcctg cccctgtgga ccaactggcg
      781 tgtgggacct gcgccgggca ttctcgactg gtacacggtg attggcggag tcgtcgcgct
      841 ggtcgccctc gccgtccacg gcgtgctgtg gatcgtgctc aaggcgcccg gcgagctgga
      901 aacccgcgcc cgccgcgcgg tcgtgcccct ctggaccgca ctcctcgtca tcaccctcat
      961 cagtctgccc gctaccatcg ccgtacggcc ctccgcgctc gaaaactaca aggcttatcc
     1021 ggttcttttc ctgattcccg cgctggtgat tgccaccctg atcgggattc tgaccttcac
     1081 aaagcagaag aagccgctgg cggccttcct gtcctcctgc ctctatctgg ttttcatgct
     1141 ggtcggcgcc gccgccggac tctatccgac gctgctgccg tcttccagcg atccatcgcg
     1201 caacatcact atcgccagcg cgatcgccgg accccattct ctccatgtgg gattgatatg
     1261 gtggtcgctc ggcctcatcc tcgccctcgc ctacttcatc acctcttact ggctcttccg
     1321 cggaaaagtg tccgcagagg ctgggtacgg acactgaaca ggattcgaag gagaacttct
     1381 cacatggcat ccgccaccgt cgcccccgat acgccgctca tcatcgatcc ggcacacgat
     1441 ctggtcagca tccagcacag tccgctggcc tcgatcttca ctccaaagtc ggtggctctg
     1501 atcggcgcaa ccgagcgcgt gggttcggtc ggccgcagcg tcatgatgaa tctgaccagc
     1561 agttctttcg gcggacccgt ttggcccgtg aatccgaaga gccccaccat ccttggcatt
     1621 ccggcataca aatccatcaa agaactccct ggcgtccccg acctcgtcgt cgtcaccacg
     1681 ccggcggcga ccgttcccgg catcattcag gaagctgtcg acctcggcgt gccatcagcg
     1741 gtcgtgatct ccgccggctt caaggaggtc ggtgagtccg gcaaggcact cgagcggcag
     1801 atcgcacaga tcatccgggg ccggatgcgc ctcatcggcc ccaattgtct cggcgtcatg
     1861 aatccggtcg gcggcctcaa cgccacgttc gccaaagcca tggtccccgc gggccgcctc
     1921 gccttcatca gccagagcgg cgctctcctt acggccatcc tcgactggtg cattcgcgag
     1981 catgttggac tcagcggctg cgtctccgcc ggatcgatgc tcgacatcac cttcggcgaa
     2041 ctcatcgacc actacggcaa ggatcccaat accgattcca tcgttctcta tatggagtcc
     2101 atcggggacg cgcgcagctt cctctccgcc gcacgtgagg tggcccttaa taaaccaatc
     2161 ctcgtgatca aggctggccg aactgccgcc gctgccaaag ccgccgcttc gcacaccggc
     2221 tcactgaccg gcagcgacga tgtactcgac gccgcgttcc agcgcgccgg tgttcttcgc
     2281 gttcaatcca tcagcgagat cttcaatatc tccgaagtgc tggccaaaca gccgcgccct
     2341 aaaggcccgc ggctcaccat tctgaccaac gccggcggtc caggagttct ggccaccgat
     2401 gcattgatca gcgcaggcgg cgagctgacc gaaatctcgc cggaaaccat gacgacgtac
     2461 gataacttcc tgccttcggc gtggagccat aacaatcccg tcgacattct cggcgacgcg
     2521 gagcccgagc gctacgccaa agcccttgag atcgcctcgc aggacaagaa caccgacggt
     2581 atgctcgtga tcctcacgcc tcagggcatg acgaatccca ccgaggtcgc cgagcacctc
     2641 attccctacg cccgttcgac aggcaaaccc gttatcgcca gctggatggg cggagattat
     2701 gtcgcccggg cgaacgaagt cctcgccaaa gcgggcattc ccgcctttgc gtatcccgac
     2761 gcagcggccg aggccttcgt cgcgacctgg aaacacacca gcaacctgcg cgatctgtac
     2821 gagacgccca ccacgcgctc cggttcggtt cttgacacca aacgcgacct cgccggcagc
     2881 atcgtcgacc aggtccgcgc gcgacgccgc acgattctca ccgaatacga gtccaagcag
     2941 ctgctcgccg cctacggcat tccgaccgtc cgcaccgagt tggcgacgac ccctgaagaa
     3001 gccgcgcaga aggcgctcga aatcggctat ccggtcgtcc tcaagctgca ttcagaaacg
     3061 atcactcata aaaccgacgt cggcggtgtg aaactcaacc tgcgctccgc tggcgaggtc
     3121 gtcaccgcgt tttccgacat tcaaaaatcc gtgacggaaa aggtcggcgc cgagcacttc
     3181 ctcggcgtta cagtgcaacg cttcgccaag ctggaaggct acgagatcat tctcgggtcc
     3241 agcatcgatc cgcaactcgg ccccgtactt ctgttcggca ccggcggcca actcgtcgaa
     3301 gtttacaaag accgtgctct cgcgctccca ccgctgaccg ccaccctcgc tcgccaccag
     3361 atggagcaga caaaaatctt caaggcgctc aagggcgttc gtggccgcaa accggtagac
     3421 ctcgatgctc tcgatgcgct cgtcgttcgc ttcagcgaac tggtcgtcga gcagcgctgg
     3481 atccgggaaa ttgacatcaa cccactgctc gcctcgcccg acggactcat cgcactcgat
     3541 gcgcgcgtgg tgctgcacga gcccggaacc actgaggacc agctgccgcg tccggcgatc
     3601 cgcccgtatc cggtgcaata catcgggaag tgggagtcgc gatctggcgc cgatgtgctg
     3661 gttcgtccga ttcgcccgga agacgagcct ctcatcgtcg acttccacga ccggctgtcg
     3721 gagcgcaccg tcgtccagcg ctacttcacg tcactcgcac tcagcgcgcg cacagcgcac
     3781 gagcgtctca cgcgcatctg cttcatcgac tacgaccgcg agctggctct cgtggccgaa
     3841 gttaacgacc cgaaaaaagg caagctcatc gccggcgtca gccgcatgat ccgccagaac
     3901 cacgggcaga acgctaccct cgcggttgtg atcgccgacg atttccaggg acaggggctc
     3961 ggtggtgaac tcatgcgccg cacacttgcc gccgctcgcg ccgagggcat cgagcaggtc
     4021 caggtctact tcctcccgga gaacaaccgc atgcgcaagc tctcacagaa gcttggcttc
     4081 actctgactg aagagaacgg tctgatgcgc ggtgagctta ccgtctgatt tgcaacagca
     4141 gaaaggggag acgtcttttg tctcccctcc ttttttgcga atttccctct gcactgcgga
     4201 tcgggtcggc cgctcgcagt tcagctcacg atcgacaact cccgcacagc ctccgccgaa
     4261 gcgaccagag gcagcgtcac ggtgacacac gtgccttcgc ccaggcggct gttcaccgcg
     4321 atgtcgccgc cgcaggaatc cacgatcgcc ttgcagatgg aaagtccgag ccccgcgcct
     4381 cccgtgcttc tcgcccgcga gtgatcgcca cgccagaatc gctcgaagag gtgcggcaaa
     4441 tcctccggcg cgatcccacg gccggtatcg cgtacctcga tcaccacact cgtgcccgcc
     4501 gcaatagccg cggtcaccga gccgccctcc ggcgtatgct gcacagcatt cagcatcagg
     4561 ttggccagca gggaagcgca cgcatcgcgc tccagccgtg tcggcgcagg ttccggcgca
     4621 tggacggcga tcttcacttc gcgaatctct gccaggcgct ccatgtgcac cgccacatcc
     4681 cgcgccactt cggcgacgtt acactccaca tcgccgctcg ctggttcacg ttcgaagcgc
     4741 gccagctgaa gcatgcgctg caccagctct tccatgcgcg cgcaatctgc catgcaggtc
     4801 tcaagtcctc tggcatattc gcgcgccgaa cgcggccgcg atgcgagcag ttgcagtgac
     4861 gatttgatga tcgtgacggc cgtcttcaac tcatgcgcgg catcgttcac aaacatacgt
     4921 tgctgcacaa aggcctcttc cagccgctcg gtcgccgatt ccaatgctcg tgcgagcaca
     4981 gtcaattcca ccgtggagcg tgcgctgcgc ggagcctcaa agcgcggccg cttcggcgtc
     5041 atttctccag cggcgcggct cagcgcctcc aaaggcgcca gcccgcgccg cagaacctgc
     5101 gccaaaacgc cggccgttat aagcaggagc agcaggctcg cgatcaccag aaacgccgcc
     5161 gcacgcctga ttgatccgta aacaggctcc agcggcgcgg catagaagat tgtcaccgga
     5221 cgcgcaatgc ccggacttcc gtcctccgca tcgatctgcc gcaccccatg cagaaccagg
     5281 ccgcggaacg tccttccccg cacgcgaaaa tcgtgcggca catcttttgc ggcgaaatca
     5341 tgcagcgctg cactgctcca ggacgtcgac tgcgccagcg tccggccacc ttcatcccgc
     5401 gccatccaca gatcgttgcc gcgcagatcg agcgccgcca gattaatcgc gacgctgtcg
     5461 ttgggatctt cggcatcgtg cacctggccc agcagggaat cggcgcgccc gcgcaccatc
     5521 aggtcaaagt tgcggatttg ttcccgccac agatacaaaa gcgtggcgct gctcagcccc
     5581 agtaccaggc cgcattccag cgccagcacc gccgcgatca gtctccggcg aagggagtag
     5641 cgcctcacgc cgcacctcca ctcgcgcccg tcatcagccg gtacccgcgg ccgcgaaggg
     5701 tttcgatcca gtgaccctcg ccgtcgcgat tcagcttacg gcgcagattg gagatgtgcg
     5761 cttcaatcac gttggagtga tgctcccagt tgtagtcgta cagatgctcc agcaggacct
     5821 gcttggaaat gatggaacgc ggccgatggc tcagatactc aagcgcgcga tattcggttg
     5881 gcgatagttc caccggcttg ccgccgacag tcacgctccg ctccagcgtg tcgatcacca
     5941 ggtctgccag ctcaattttc ggatggctca cgcccttgcc ccggcggatc agcgccttgg
     6001 cccgcgccag catttctccc aggtcgaagg gttttggcag atagtcgtcg gcgccggcat
     6061 tcagcagctt cacgatcgaa gtcttttccc caaccgcggt cagcaccagc acgggagccg
     6121 tctgtccttt tgcccgcatc gtcttcagca ccgcgaggcc gtccatgcct ggcagcatca
     6181 ggtccagcac gatcaggtcg tagttcgcct gccgcccaag ctccgcgcca tccaggcccg
     6241 tccgcgccac atcgacggcc aaacccgcgc tctcgcgcag ggcctccgcg atattccccg
     6301 cgacccgggc ttcgtcctca atcacaagcg cgcgcatgct gggcagcatc agccttcgcc
     6361 tccattgtgc acgaatttgg gtccgggcga gaggggcatt ttcccctgcc tgccacaaag
     6421 gcctcttgcg cccctcgcta caataaataa atgcgccgca tctatatgga cgccaatgcc
     6481 accacgcctc tgctgcccga ggtgtttgag gccatgcggc ctttctttat cgagtcgttc
     6541 ggcaacgcct cctcaatcca tcatttcggc cagcgcgccc gcgccgccgt cgagcaggcg
     6601 cgcgagcagg tggccgcgct cctccactgc cgtcccgccg aaatcgtctt cacctcctgc
     6661 ggcaccgaga gcgacaatac cgcgatcttc gggattctca aacccggcga ccacctcatc
     6721 accacctcca ttgagcatca cgccgtcctg cacgcggcgg aaaaggccga ggcgcggggc
     6781 gtggcggtga cagttttgtc cgttggcccc tccggcgtaa tcgatcccgc cgaagttcga
     6841 gccgccctga agccgaacac aaaactgatc agcatcatga tggccaacaa tgagaccgga
     6901 gtcgtccagc ctgtcgaagc gatcggaaaa atcgcggccg aggccggcgt tacatttcat
     6961 accgacgctg tgcaggccgc cggcaaaatc ccgatcgacg ttgcagccat cggttgtcac
     7021 ttgctcagta tctccggcca caagtttcat gggccgcagg gcatcggcgt cctctacgtg
     7081 cggcgcggaa cccatttcga gccgctcgtc ttcggtggat cgcacgagcg ccagcgccgc
     7141 gccggaaccg aaaacgttcc cggcatcgtc gggctcggga aagctgcgga aattgccctc
     7201 gccgcgatta cggacggcac cgacgagcgc gtcggccatc tgcgggatcg cctggaacaa
     7261 ggtctgctgg cacgcgtcga cgaatgcggc atcaacggcg ccgatcgatc gcgggttccc
     7321 aacaccacca acatctactt cgatcatctc gaaggcgaag cgctggtcat cgcgctcgac
     7381 ctgaagggcc ttgcgtgttc gggcggatcc gcgtgcagtt ccggttcgcc ggagccgtcg
     7441 catgtgctca attcgatggg aatctcacaa gcccgcgcgc gcgccagcct gcgcttcagt
     7501 ctttcacggc tcacgaccga cgaagacgtc gacgcggcgc tggatctcgt tcccggtgcc
     7561 gtcgcgcatc tgcgcgaact ctccccggtc tacaaaaagc acctcaccga agctgccgcc
     7621 caatagaatt tcctgttcgc aaaatcatcc gaaggagcca aattgaagcg gcgtagcttt
     7681 ttcagaatgg cggccggcag tgccggcatg ctgtggacca gtggactgga gcgatcgctc
     7741 caggcactcg cccagggccc agcctcgtca gccgtccgtc gcgtcctggt gatgttcaag
     7801 tgccactttg acgccggatt catcgacacc cagaccgccg tcgtccacaa atacttttcg
     7861 caatattttc cgcaggccat ccgcgtcgcg cagcagtctc gcgaacccgg ccagcatcgc
     7921 tacgtgtgga ccaccggc
//
LOCUS       DASZYN010000045         2809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_130663, whole genome shotgun sequence.
ACCESSION   DASZYN010000045 DASZYN010000000
VERSION     DASZYN010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130663"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /gene="trxA"
                     /locus_tag="VHE33_01295"
     CDS             complement(<1..305)
                     /gene="trxA"
                     /locus_tag="VHE33_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HVT96110.1"
                     /translation="MAALGIAEVTDATFDQEVLQSEQPVLVDFWATWCGPCRALAPIV
                     DEVATEYSGRLKVAKMDVDHNQATPMRYGIRGIPALLIFKGGKVADQIVGYVPKETIA
                     "
     gene            complement(650..1537)
                     /gene="thrB"
                     /locus_tag="VHE33_01300"
     CDS             complement(650..1537)
                     /gene="thrB"
                     /locus_tag="VHE33_01300"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HVT96111.1"
                     /translation="MKLRLPATSANLGPGFDALALALKLYLEVEAEPAEQMSIEATGR
                     NADICGATENNLLLSTYGETLAAHGRPIVPLRLQVRNEIPLGMGCGSSAAVRLAGVAL
                     AAHFGELGWSGERVLAEASRLEGHPDNAAACWLGGFVASAMEGGQVRAISIAAPREWR
                     AVIAMPEEPLGTHASRGFLPEAYTRKDVVENLQRVGLLTTAFATGKAEMMAVAMGDRL
                     HQPYRAEACPLLDRLLPLAGRGGIAGVALSGAGPGVLLLTNSQQAALEAKNLAAQAVQ
                     GLGKVELLVAELENEAVRF"
     gene            complement(1534..>2809)
                     /gene="thrC"
                     /locus_tag="VHE33_01305"
     CDS             complement(1534..>2809)
                     /gene="thrC"
                     /locus_tag="VHE33_01305"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HVT96112.1"
                     /translation="FRCADCGELFEVVYPSWTGPKQTRPNPSALRWLWKERRMSMEMA
                     DRSGVWRFREMLPILGSEKSIVSIAEGNTPLYDLRRAGERLGVARLHAKHQGMNPTGS
                     FKDTGMTTAISVARESGATWVACASTGNTSASMAAYAARAGMRSLVLLPDGEIAWGKL
                     AQAMDYGALTLQLKTDFDGCMRVLMEVLRRAPVYLLNSVNPYRLEGQKTAAFEVAEQM
                     DWQVPDHLIVPGGNLANSSALGKGFLEMKQLGLTAKTPRISVIQAEGANPLVRSMSEF
                     GGKKLEPVEAKTRATAIRIGNPASWKKAVRVIEETGGWVEQVSEAEIALAKAEIGAEG
                     IGCEPASAVTLAGLKKLIAQEKVGAEDSVVLLLTGHTLKDPDYTIQYHRGEWPKEAPA
                     ELTSAMAGTRRNAVRIEASVDAVLSELERFGR"
BASE COUNT          512 a         1114 c          674 g          509 t
ORIGIN      
        1 gcgatcgtct ccttgggcac atacccaacg atctggtccg ccaccttgcc gcccttaaag
       61 atcagcagcg ccggaatccc ccggatcccg taccgcatcg gcgtcgcctg gttgtggtcc
      121 acatccatct tcgccacctt cagacgcccg ctgtactcgg tggccacttc gtccacaatc
      181 ggcgccagcg cccggcacgg tccgcaccac gttgcccaaa agtcaaccag aaccggctgc
      241 tccgactgca gcacctcctg gtcaaacgtc gcatccgtca cttctgcgat ccctaatgct
      301 gccatctctc tcgttgtccc ttcggcgcgg tcgcgcgcct cctcagttta ctcgaccaca
      361 aattagatgc agggtggatt cgcgggactc acgccaggct gatacggcac cgtttccagt
      421 gcaaaaagtt aaggcgcccg gatcctttaa ggaatcacgg acgccggagc agccctcccc
      481 cagaactgcc cacagatgcg aaaatagggc ttcagatgct tttcggcaag caatcttcgg
      541 taatttttgg taatagactc aacgagttac gctgggacac agaaatgcat tacttttggg
      601 tcatatgctc ggcagccgct ccacacaccc atgcaccggc cccagaagcc taaaaccgca
      661 ccgcctcgtt ctccaactcc gccaccagca actcgacctt ccccagtccc tgcaccgcct
      721 gagctgccag attcttcgct tccaaagccg cctgttgtga attagttaac aggaggaccc
      781 cagggccagc accgctcagc gcaacccccg caattccacc ccgtcccgcc agcggaagca
      841 gccgatccag caggggacaa gcctcggccc gatacggctg atgcagccga tcccccatcg
      901 cgaccgccat catctcggcc ttccccgtcg caaacgccgt cgtcagcagc cccacccgtt
      961 gcagattctc cacgacatcc tttcgtgtgt aagcctcggg caggaacccc cgcgaagcat
     1021 gcgtgcccag cggctcctcc ggcatcgcga tcaccgcccg ccactcccgc ggcgccgcaa
     1081 tcgaaatcgc ccgcacctgc ccgccctcca tcgcgctcgc gacgaaccct cccagccagc
     1141 aagccgccgc attgtccgga tgcccctcca gccgcgaagc ctccgccagc acccgctccc
     1201 cactccagcc cagctcgcca aaatgcgctg ccagcgccac gccggccagc cgtaccgccg
     1261 ccgacgaccc gcagcccatc cccagcggaa tctcgttcct cacctgcagc cgcaacggca
     1321 caatcggccg cccgtgcgcc gccagagttt ccccgtaggt cgacagcagc agattgttct
     1381 ccgtcgctcc gcagatgtcc gcgttgcgtc ccgtcgcttc aatggacatt tgctcagccg
     1441 gctccgcctc cacttcgagg taaagcttca gagcaagcgc cagcgcatcg aaacccggtc
     1501 ccagattcgc cgacgtcgcc ggcagccgca gcttcatcgt ccgaaccgct ccagttcgct
     1561 cagcaccgca tccaccgacg cctcaatccg caccgcattc cgccgcgtcc ccgccatcgc
     1621 agacgtcagc tccgccggcg cctccttcgg ccactcgcca cggtggtact ggatcgtgta
     1681 gtcgggatcc ttcagcgtgt gtccggtcag cagcagtacc accgaatcct ccgcgcccac
     1741 tttctcctgc gcgatcagct tcttcagccc cgccagcgtc accgccgatg ccggctcgca
     1801 gccaatcccc tccgctccaa tctccgcctt cgccagcgcg atctccgcct cggagacctg
     1861 ctcgacccac ccgcccgtct cctcgatcac gcgcaccgcc ttcttccacg atgcgggatt
     1921 gccgatgcga atcgctgtcg cccgcgtctt cgcctctacc ggctccagct tcttcccgcc
     1981 aaattcgctc atcgaccgca ccagaggatt ggccccttcc gcctggatca ccgaaatcct
     2041 cggcgtcttt gccgtcaggc ccagctgctt catctccaga aagcctttgc ccagcgccga
     2101 actatttgcc aaatttcccc ccggcacaat caaatgatcc ggcacctgcc agtccatctg
     2161 ctccgcaacc tcaaacgccg cggtcttctg gccctccagc cgatagggat tcaccgaatt
     2221 cagtagataa acaggcgcgc gtcgcagcac ctccatcaac acgcgcatgc atccgtcgaa
     2281 gtccgtcttc agctgcagcg tcaacgcacc gtaatccatg gcctgcgcca gcttgcccca
     2341 cgcaatctct ccatcgggca gcagcaccag gctccgcatc ccggcgcgcg cggcataggc
     2401 tgccatcgat gccgacgtgt tccccgtgga tgcgcaggcc acccacgtcg ctccactctc
     2461 tcgcgccaca gagatggccg ttgtcatgcc tgtgtctttg aacgaaccgg tgggattcat
     2521 cccctgatgc ttggcatgca gccgcgccac ccccagccgc tcgccggctc ggcgcagatc
     2581 gtagaggggc gtattgccct ccgcaatcga aacaatgctc ttttccgaac ccagaatcgg
     2641 cagcatctcc cggaaacgcc acaccccact gcggtcggcc atctccatcg acatgcgccg
     2701 ttccttccac aaccagcgca gcgcgcttgg gttgggccgc gtctgttttg gccccgtcca
     2761 gcttggatac accacttcga acagctcgcc gcagtccgcg cagcggaag
//
LOCUS       DASZYN010000046         3790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_964630, whole genome shotgun sequence.
ACCESSION   DASZYN010000046 DASZYN010000000
VERSION     DASZYN010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964630"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VHE33_01310"
     CDS             complement(<1..224)
                     /locus_tag="VHE33_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96113.1"
                     /translation="MHRFVAVAVPGLLLGAATLLVAGCGSSSGAAPKPQPDFSLSASA
                     STLTLTAGGSAQAVSLTAMALNGFAGTIQAT"
     gene            complement(276..890)
                     /gene="rsmG"
                     /locus_tag="VHE33_01315"
     CDS             complement(276..890)
                     /gene="rsmG"
                     /locus_tag="VHE33_01315"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HVT96114.1"
                     /translation="MNQRDEHQSALKALGLDSPSVAQLETFLALLLRWNARINLTAVR
                     QPEEIIRRHFAESIFAAQQIPNRTKTLLDYGSGGGFPGIPIAIRRPDIAVTLAESQNK
                     KAAFLREAVRTLGLKAEVWADRVEAMEPGRVFDGITLRAVDKMPAACRIAAERLAPRG
                     WIAVFTTRRTEGALEEIPGVCWDAALEIPGSEQEILKTGHRSPE"
     gene            complement(916..1662)
                     /locus_tag="VHE33_01320"
     CDS             complement(916..1662)
                     /locus_tag="VHE33_01320"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HVT96115.1"
                     /translation="MKAPRIAIPEPNFADREYSERSWPQYARAVEFAGGEPVKVPLRD
                     SPAKVAQLVASCEGVLLPGSPADVNPQKYGEEADPDCAAADHAREAVDELLLQDASNL
                     YKPLLGICYGFQALNVWRGGTLVQHLKTAVNHAAGKTVTEAHPVEFDTRSRLRRILGL
                     GSRNTMMVNSSHHQAIGILGDGLIKSAWSPDDGVVEAAEVHGDQFLVGVQWHPERLLE
                     ESKAAQCLFQCFVDAAAEWEPRMIRESVAD"
     gene            complement(1659..2537)
                     /locus_tag="VHE33_01325"
     CDS             complement(1659..2537)
                     /locus_tag="VHE33_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVT96116.1"
                     /translation="MKPTVPTAPRPNPLPLHERFISNVFLFPTFVVVTAFFASLAVLA
                     SLFEKRGYWQHRIAQTWARVSVLISGSRVTVCGREHAVGRPAVYICNHLSYMDTPVIF
                     SSLPFQFRIVARHDLWKLPFIGWWLNRSGQVPVDVSNPRASISSLIGAVRTLKSGMPL
                     FIFPEGGRTPDGKMAKFMNGPAFMAIHAQLPIVPMALIGTHELLPIHTHTIHKVPVTL
                     AIGEPIETQGLTTKQVNELTTRLEQRVAALYDEHSWQKRAAAPPVNGEGAAGVGHLEA
                     VGIARDVQATEEARRQ"
     gene            complement(2534..>3790)
                     /locus_tag="VHE33_01330"
     CDS             complement(2534..>3790)
                     /locus_tag="VHE33_01330"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HVT96117.1"
                     /translation="LGHPEQRFRSVLIAGTNGKGSTAATLSAILRSAGYRVGLYTSPH
                     LERVNERIRINGSPISDEDFARLYFRVDDCANRLVAEGRLPAHPSYFESLTAVAFEAF
                     AEAQVDLAVLEVGMGGRLDATNVVEPLLSVITDISLDHMEWLGNTITEIAREKAGILR
                     PNGVLVTLPQHPEANQAIGEVAMGLNVRGVSAADYIPSIQHSAFSVQGSGPTRNQYIL
                     QVLDQPITIDSPLAGVHQQRNIALAIATAVELRNDHGYKITPAAIEDGIRLTSWPGRL
                     EQFQIPGKAPVILDVAHNPAGAWALRAALSALRPELCALRSVLAFGCMRDKAWEEMAQ
                     ILFPLFDQVIAVPVPSPRSADPADLLRAAEKCGVPAIAARDGKEALELAWREAGAEGV
                     VVIAGSVYLVGEIRPLLHPHTKGSAQ"
BASE COUNT          714 a         1124 c         1264 g          688 t
ORIGIN      
        1 gtggcctgaa tggttcccgc gaatccgttc agagccatag cagtgagcga aactgcttgc
       61 gccgaaccgc cggcagtcag agtcagggtg gaagcgctgg cgctgaggga gaagtcgggc
      121 tgcggctttg gggcggcgcc ggaactggag ccgcaaccgg cgacgagcag ggtcgctgca
      181 ccgagcagga gaccgggcac tgccacagca acaaaacgat gcatgcgagc cctcccaggg
      241 ggatggtttc gaagagccgg cgaattctcc taacgctact ccggcgaacg atggcctgtc
      301 ttcaatattt cctgttcgga accgggaatt tcgagcgcag cgtcccaaca aaccccggga
      361 atttcttcga gagcgccttc ggttctgcgg gtggtgaaca cagcgatcca gccgcgagga
      421 gccaggcgtt cggcggcaat tctgcaggcc gcgggcattt tgtcgacggc ccgcaaggtg
      481 atgccgtcga agactcggcc cggctccatc gcctcgacgc gatcggccca gacctcggct
      541 ttaaggccga gtgtgcgcac agcctcgcgt aggaacgcgg cttttttgtt ctgcgattca
      601 gcgagtgtga ccgcgatgtc cgggcggcgg atcgcgatgg ggatgccggg gaatccgccg
      661 ccagatccgt agtcgagcag ggtctttgtt cgattcggaa tttgctgcgc cgcgaagatc
      721 gattctgcga agtgccgccg gatgatttct tccggttgcc gcactgccgt caggttgatg
      781 cgtgcattcc agcggagcag cagagcgaga aaggtctcca gctgggcgac gctgggtgag
      841 tcaaggccca gagccttgag cgcagactga tgctcatccc gctgattcat ggttggtcgg
      901 ttcaggcgcg aaagtctagt ccgcgacgga ctcgcgaatc atgcgcggct cccactccgc
      961 ggccgcatcg acgaagcact ggaagaggca ctgggcggct ttgctttctt caagcaggcg
     1021 ctccggatgc cactgcacgc ccaccagaaa ctggtcgcca tggacctcag cggcttcgac
     1081 cacgccatcg tccggagacc atgccgattt gatgagcccg tcgcccagaa tgccaatcgc
     1141 ctgatggtgg ctgctgttga ccatcatggt gttgcgagag ccgagaccga gaatgcgacg
     1201 cagacgagag cgcgtgtcga actcgaccgg gtgcgcttcc gtgacggttt tgccggcggc
     1261 gtgattcacg gcggtcttca gatgctggac cagtgtgccg ccgcgccaga cattgagcgc
     1321 ctggaacccg taacagattc cgagcagcgg cttgtacaga ttcgaggcat cctgtaacag
     1381 cagctcgtcg acggcttcgc gggcgtggtc ggcggccgcg cagtcagggt cggcttcttc
     1441 gccatacttc tgtggattca catcggcggg gctgccggga agcagcacgc cttcacagga
     1501 ggccaccagc tgcgccactt ttgcgggcga atcgcgcaac ggcaccttca ccggctcgcc
     1561 gccggcgaac tccaccgcgc gtgcatattg aggccacgat cgctcgctgt attcgcgatc
     1621 ggcaaagttg ggttccggaa tcgcaatgcg tggtgctttc attgtctgcg cgcctcctcg
     1681 gtggcttgca cgtcccgagc gatgccaacc gcttccagat gcccaacgcc tgcggcgccc
     1741 tccccgttta ccggcggcgc tgcggctctt ttctgccagg agtgctcatc atacagcgca
     1801 gcgacgcgtt gctccagtcg cgttgtcaac tcgtttacct gtttggtggt cagcccctga
     1861 gtttcaatcg gctcgccgat ggcgagggtc accggaacct tgtgaatggt gtgggtgtgg
     1921 atcggaagca gctcatgcgt tccgatcagg gccataggaa cgatgggaag ctgggcgtgg
     1981 atggccatga aggcgggacc gttcatgaat tttgccatct tgccgtccgg agtgcggccg
     2041 ccttcgggaa aaataaagag cggcatcccg ctcttaagcg tgcgcacggc gccgatgagg
     2101 ctggatatgg aggcgcgcgg attcgagacg tcgaccggga cctgtccgga gcggttcagc
     2161 caccacccga tgaaaggcag cttccacaga tcgtggcggg ccacaatgcg gaactggaag
     2221 ggcagcgaac tgaagatcac cggcgtatcc atataggaca ggtgattgca gatgtaaaca
     2281 gcgggccgcc ccacggcgtg ctcgcgaccg cagaccgtaa cgcggctgcc gctgatcaac
     2341 acgctgacgc gagcccaggt ctgcgcaatg cgatgttgcc agtaaccgcg tttctcaaaa
     2401 agcgatgcca gaaccgcgag gctggcgaag aacgcggtaa cgaccacgaa cgtggggaag
     2461 agaaaaacgt tgctgatgaa tcgctcgtgc agtggcagcg gattcggcct gggcgccgtg
     2521 gggaccgtgg gcttcattgc gccgatccct ttgtgtgcgg gtggaggaga ggccgaattt
     2581 cgccaaccag gtagacagag ccggcgatga ccaccacgcc ctccgcgccg gcctcgcgcc
     2641 aggcgagttc cagagcctcc ttgccatcgc gggctgcgat ggcgggaacc ccgcactttt
     2701 cggcagcgcg cagcaggtcg gcgggatcgg ccgatcgcgg agatggcacc ggcacagcga
     2761 tcacttgatc gaagagcggg aagaggatct gggccatctc ttcccaggct ttatcgcgca
     2821 tgcaaccaaa ggccaatacg gagcggagag cacagagctc agggcgcagc gccgaaagtg
     2881 ctgcccgaag agcccaggcc cccgcggggt tgtgggcgac gtcgagaatt accggcgctt
     2941 tgccgggaat ttggaactgc tccaggcggc cgggccagct tgtaagacgg atgccgtctt
     3001 cgatagccgc cggcgtgatc ttgtatccgt gatcgttacg taattcgaca gccgtggcaa
     3061 tcgccagcgc gatattgcgc tgctgatgca ctcctgcaag cggcgagtcg atcgtgatgg
     3121 gctggtccag aacctgaaga atgtactggt tccgcgtcgg acccgagccc tgaacgctga
     3181 acgctgaatg ctgaatgctc gggatatagt cggcggcgct gacaccgcgg acgttcaggc
     3241 ccatcgcaac ttcgccgatg gcctggttgg cctccgggtg ctgcggcagc gtaaccagca
     3301 cgccgtttgg gcgcaggatg ccggccttct cgcgtgcgat ctccgtgatg gtgttgccga
     3361 gccactccat gtgatcgagc gagatatcgg tgatgacgga gagcagcggc tcgacgacgt
     3421 tggtcgcgtc gagacggcca cccatgccca cttcgagcac cgcgagatcg acctgcgcct
     3481 cggcgaaggc ttcgaaggcc acagcggtca gggattcgaa gtagctggga tgagccggga
     3541 gccggccctc ggccaccagg cgattggcac aatcatccac ccgaaagtag agccgggcaa
     3601 agtcttcatc gctgatgggt gagccgttga tacggatgcg ctcgttgacc cgctccagat
     3661 gcggcgaggt gtagaggccg acgcgatagc cggcactgcg cagaatcgcg gacagggtcg
     3721 cggcggtgga acctttgccg ttggtgccgg caatcagaac ggagcggaag cgctgttcgg
     3781 gatggccgag
//
LOCUS       DASZYN010000047         2322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1030038, whole genome shotgun sequence.
ACCESSION   DASZYN010000047 DASZYN010000000
VERSION     DASZYN010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030038"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(427..1566)
                     /locus_tag="VHE33_01335"
     CDS             complement(427..1566)
                     /locus_tag="VHE33_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96118.1"
                     /translation="MAQVSSIPNLEPVAPAARLIGSVRVLIQFDICEEIRLDQLQQII
                     NSRTVQKPNLKHAGPAYVRYERPPVVVAADPLVLDSGENLAGEIKYYDYGVVSLLFEI
                     PFAGDWDDLIRLASRWVWDIAFASRVEPILRQRLERASSTLVKPYSRWLSEDYFIFHI
                     REVAREAPDQPSAAELVRTSGRQIAQVVRGDPQPLSDGECAEVLNSQISYYASDVAVI
                     GWNAAFLYDTAAGAETAIQLLEYANSQLLEFRHYDELLTGVLDSVYDTLEQKRGRLAR
                     WRMSRSANSLHTVLLEVSELTEHADNAIKFLSDMYAARLYRLAAAKVGVPDYKNLVTQ
                     KLRTAEELYDYMVEQFNQSRGFFLEVIVVLILLIELVFLFRGQPS"
     gene            1906..>2322
                     /locus_tag="VHE33_01340"
     CDS             1906..>2322
                     /locus_tag="VHE33_01340"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVT96119.1"
                     /translation="MQPRFAQGTCIAVKASDRYPFLVMGKVLAVVNQKGGVGKTTTAI
                     NLSAALALEGLRVLLVDCDPQANSSGGFGVARDADRRSSYDVLMGDAGIAEVALRTEI
                     DTLKLVPSHKNLTGANVELIQMDRRAFKLKDAIDKVR"
BASE COUNT          466 a          570 c          839 g          447 t
ORIGIN      
        1 ctggttcgcc tcggtaatac ggcccttgag atcggtacgg gagtagatga agaccccttc
       61 ggggagcagc gtttcaacgc tggtggtatg aaccttgata gtcgacgcag ccatgacagc
      121 cctcgacgtc tgcatcggca ggaacggggc ggacttgagg gcgcgggggg gaggcacggg
      181 tgccgcggcc ggcgttctcc cggtccctga ccgttccaac ttccctcggc gtctgaagcc
      241 gctcgcaatc gaagcgattt ttcggcacgg ctgaaagagc ttgcagaaaa agtcgagagg
      301 gcggaagtgg ggacggggtt tttcccaaaa cggaccaggg cggtacccct ggatgggtaa
      361 tttggaatcg aagaatatgt aacaactgtt gttacatatt cttcagggca cgaccggcac
      421 acgcctctat gacggctggc cgcggaagag aaagaccagc tcgatgagca ggatcaggac
      481 cacgatgacc tcgaggaaga agccgcggct ctggttgaac tgctcgacca tgtagtcgta
      541 aagctcttcg gcggtacgga gcttttgggt gaccaggttt ttgtagtcgg ggacgccgac
      601 tttggcggcg gcgagtcgat agaggcgggc ggcgtacatg tcactgagga acttgatggc
      661 gttgtcggcg tgctcggtga gttcggagac ttcgagcaga acggtatgga ggctgttggc
      721 cgagcgggac atgcgccagc gggccaggcg gccacgtttt tgctccaggg tgtcgtagac
      781 gctgtcgagg acgccggtga gcagctcgtc gtagtggcga aactcgagga gctgggagtt
      841 ggcgtattcg aggagctgaa tggcggtttc ggcgccagcg gctgtgtcat aaagaaaggc
      901 cgcgttccat ccaatgaccg ctacatcgct cgcgtaatag gagatttggg agttgaggac
      961 ctcggcgcac tcgccgtcgg agaggggttg gggatcgccg cgaacgacct gagcgatctg
     1021 gcggccggag gttcggacga gttcggcggc ggagggctgg tcaggggcct cgcgggcgac
     1081 ctcacgaata tggaagatga agtagtcctc gctgagccag cgggagtagg gcttgacgag
     1141 ggtcgaggag gcacgctcga ggcgctggcg gaggatgggc tcaacgcggg aggcaaaagc
     1201 gatgtcccaa acccagcggc tggcgaggcg gatgaggtcg tcccagtcgc cggcgaaggg
     1261 gatttcgaag agaaggctga cgacgccgta gtcgtagtac ttgatctcgc cagcgaggtt
     1321 ttcgccggaa tcgagaacga gaggatcggc ggcaacgacg actggggggc gttcgtaccg
     1381 gacataggct ggcccggcgt gcttgaggtt gggtttttgg acggtacggc tgttgatgat
     1441 ttgctggagt tgatcgaggc ggatttcctc gcaaatatca aactggataa ggacgcggac
     1501 ggacccaatg aggcgcgccg ctggggcgac gggttcaagg ttcgggatgg aggagacctg
     1561 ggccatcgga cacgcaggga ggggcaagga actgctgtcg ttatacgctg agcggggttt
     1621 ggggcgaaag ccagtgttcc acgtggaaca cgtgcagggt gaaggtgcag ccgaaagcga
     1681 acagccggta gtgttccacg tggaacatgg accggccatc aaggcgcagt tgggttacgc
     1741 cggaatgctt cgcagggcgg gacttttcgc cgggtgcgta ccgtgttttc cacgcaggcc
     1801 tcaagaggtg caaccctttg agctaaattc cacccagccg ctatcttgcg gtgttgactg
     1861 gagttacaac aggctcctgc cgggcgatct ggcgaacttc cacaaatgca gccccgtttc
     1921 gcacagggga cctgcattgc ggtgaaggcg tctgaccggt acccttttct ggtcatgggg
     1981 aaagtcctcg cagtcgtgaa tcagaaaggc ggggtgggca agacgaccac cgccattaat
     2041 ttgtcagccg cactggccct cgaagggctg cgggtattgt tggtggactg cgatcctcag
     2101 gccaattcca gcgggggatt tggggtagcg cgggacgccg atcggcggtc ttcgtacgac
     2161 gttttgatgg gggatgcggg aattgccgag gtggcgctcc ggaccgagat cgatacgctg
     2221 aagctggttc cctcgcataa gaacctgaca ggcgcgaatg tcgagctgat tcagatggat
     2281 cggcgggcgt tcaagctgaa ggatgcgatc gataaggttc gc
//
LOCUS       DASZYN010000048        15388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_258950, whole genome shotgun sequence.
ACCESSION   DASZYN010000048 DASZYN010000000
VERSION     DASZYN010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 15388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15388
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258950"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VHE33_01345"
     CDS             <1..708
                     /locus_tag="VHE33_01345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT96120.1"
                     /translation="RIEIRPHSGQGEEVDGFHPKGGAVLHRLDLSAPADQLFRSFHKD
                     CIQRKVRRAERENLAYEEGSSEAQILDFYRLMTMTRRRHGLPPQPLQWFRELVAAFGP
                     NLKIRIARKDGAAVASIITLSHRQTMVYKYGCSDASANKYGGTPMLFWQTIQDAKSNG
                     QTELDMGRSDLEDPGLSVFKEHWGSIPTPLTYWKFPAEAGRTRPQWQKSLANRVIESS
                     PDRLLQLAGNVLYRHMG"
     gene            763..2913
                     /locus_tag="VHE33_01350"
     CDS             763..2913
                     /locus_tag="VHE33_01350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT96121.1"
                     /translation="MLTRLYYLVKPILPWRLRVSMRRWRAERRRRQYADVWPIDPGSD
                     AVPPRWPGWPDGKRFGLVLTHDVEGKKGYDRVPRLVELTQKYGFRASFNFVPHGEYEV
                     TPAMLQLLENAGFEAGVHGLQHDGKLYQSPKKFAAKAAQIREILHQWGAVGFRSPLMQ
                     HRLGWMHELGCEYDCSTFDVDPFEPESDGMGTIFPFWVQAPGSEGFVELPYTLVQDFN
                     LFKVLGESSIDIWKKKLDWVAEHGGMALLNTHPDYMTMDGSPDRDEYPVARYEEFLRY
                     ARDKHGDQCWHALPREVSRHYCDNVPAESRNTRRRICMVAYSGYETDGRIRRYAEALA
                     RRGDMVHVIALDGLSSGKKVTTLNGVTIYHVQRRDHNESGQWSYVFRMVRFLFRSAAV
                     LTRLHARNRYDVVHIHNMPDFLVFAAWYPKMTGARLILDIHDVVPELFANKFATGLKK
                     VYVAGLRVVEKLSCHFVDHVIISNHLWVEKLLGRSARPGQCSVVVNLPDPEVFYRRQR
                     TRQDDRYIVIFPGTFQWHQGLDIGIRAFAELRKRMPNAEFHLYGAQHEKLMNWLKGIA
                     QELGLEDSVKFCGIAALDRIPEIIANADLGVVPKRADSFGNEAYSTKIMEFMSQGIPV
                     VVSRTKIDSYYFDDETVRFFPSGDSAAMAEAMYEVAQNPTLRDSLVRRGLEYVELHGW
                     DRTKQQYFDLIDGLSTQKFDAYDLKRGVTPVVEG"
     gene            2997..4103
                     /locus_tag="VHE33_01355"
     CDS             2997..4103
                     /locus_tag="VHE33_01355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT96122.1"
                     /translation="MSSGTTIVEVDPWKDPRWENFVVRHPDATIYHHPAWLKVLEREY
                     RQRSLFLACQDRDGTMQGVFPLLYTRGLPFSRGPLTGPRLSSLPRTPLAGPLAVDHAV
                     RCALVREALQRATQHSVRLQIKLETEQLNGGIEGIVRKAWRESYIVPLAGKPGQPYHI
                     PGSQNRSSIKRSINKAIANGVRTRPAETEDDLRVWYRLYLETMRRNFVPARPYRFFLG
                     LWELMRGSGMMRLLLAEQQTPEGARIIGGHLFFAFGSTVTYAFGASRSADFGVRPNDI
                     IMGQAINDASEAGYKFVDLGEVPDGDNDLARFKSKWGAEPVRLYRYYFPDFPDSDHSS
                     DKAYGNAFIFARKVWNHLPLGLTSWLGDRIYTYL"
     gene            4186..5121
                     /locus_tag="VHE33_01360"
     CDS             4186..5121
                     /locus_tag="VHE33_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVT96123.1"
                     /translation="MEVQSSPEKAPEIESGARPLSAQPAHRFTRRSFLTLAASSLGGL
                     AVYSGEIARHHLTVENYTVRLPRLPDSFRGMRIAQISDIHYFEWTESFYLRQVVDHVN
                     ALKPDLVVLTGDFVTYGPVRWPSQDAHKRFALRHMPECASILSGLRCPLRYATLGNHD
                     MMVGGRYVYGDLVNQGLPVLRNSAVPLERDGQRLWLAGLGSACARDADPERAFPASSV
                     RDREAMIVLAHEPDILPEIAKYNADLMLAGHTHGGQLCIPFLRPAYLPEYGKKYVRGW
                     FRSGSTQLYVNRGVGTIGVPLRVNCPPEITLLTLA"
     gene            5365..5439
                     /locus_tag="VHE33_01365"
     tRNA            5365..5439
                     /locus_tag="VHE33_01365"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5398..5400,aa:Gly,seq:tcc)
     gene            5549..6853
                     /locus_tag="VHE33_01370"
     CDS             5549..6853
                     /locus_tag="VHE33_01370"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HVT96124.1"
                     /translation="MANSVLAISSDLHRAAKAAESKGLTHSDGRIPVLDGIRGIAILL
                     VMTFHFWIVGIAGNSTSSSISTGAFSWEHIYNYAAGMGWLGVDLFFVLSGFLITGILL
                     DSRESPRYFRVFYGRRTVRIFPLYYAALTVFFLLGPFVMAHVYRPALAEMQSGGTVKL
                     FAWTYLLNWYEGFRGWSAVAHPLQHFWSLSVEEQFYLIWPFLVLKLARRRLMGVCVGL
                     MGAALTLRAILYWLHLPFAAYLWTICRADSLAMGAIVALAARDAGDWDLFLKWARRLA
                     VPLLAALVLGRIFNPRCTVGPGDTPTLFMNTFELTLAGLFFGVWIGLAVASHNGNPAH
                     RFLASPFLRFFGKYSYCLYICHLPIIVVLARAGLNSAHLAGKLHSQLLAVLAVDGIAL
                     AVSTMIAYASWHLYEKQWLKLKNLHGMQREQLLPAQLAVQDR"
     gene            6953..7918
                     /locus_tag="VHE33_01375"
     CDS             6953..7918
                     /locus_tag="VHE33_01375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVT96125.1"
                     /translation="MSEPLFSIVIACYNHERFIRDAVESALRQNASREVIVVDDASAD
                     GSAEVVRSFGASVTFERLSVNRGAAAARNHGASVAKGKYLIFLDGDDALTPWTLKVYG
                     RVIEERGAKLILGRSSLFYGDLPATAEAPGKIRFVDYATFFDKDRPWVYNTSSLVVER
                     EAFQQAGGWSEAIFYQDIQDLLNKLCIAGKTSMVLAPDTVFYRMHSTNAVRQVTPFIE
                     GINTLLAKARRGIYPGDRAVQKKRAVWFGGLIFYWAKEGLRNGHLRDGLKLLFFNWWM
                     VLLAVIRRVIARIGGRRPIEVLTLEPNFTENSVGSAHARTVTEHP"
     gene            7982..9172
                     /locus_tag="VHE33_01380"
     CDS             7982..9172
                     /locus_tag="VHE33_01380"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HVT96126.1"
                     /translation="MNIGIIGCGFTADHYLFCLQWHPELKVVGATDRDPERASKFCNL
                     HKVKLYPTLEDMLSDGSIDMVLNLTNSSSHYETSKACLNAGKHLFTEKPLATDFSQAK
                     ELVELADAKGVYFSAAPCNLLGETAQTLWKALRNKEIGDVRLVLAELDDGPFHLAAPH
                     TWQSESGAPYDYREEFKMGVTVEHSAYYLGLFAAFFGPARTVTPFSAVLWPERRISSD
                     ETLQLTTPDFSVACITFESGVVARLTCSLVAPFNHVLKITGDTGVITVNEVWNYTAKV
                     YVDKYTSMRYRAERYSITKEHPVIANMVGNHKVYPPVRKANLKKRYARYHMDYGRGIA
                     DLALAIQQQRPPRIPADFCLHLTELGLAIQKAEPGPYQVKTTFKPLQPLDEAGLNELI
                     PAKW"
     gene            9330..10421
                     /gene="rfbB"
                     /locus_tag="VHE33_01385"
     CDS             9330..10421
                     /gene="rfbB"
                     /locus_tag="VHE33_01385"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR01181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HVT96127.1"
                     /translation="MNATDANGTILVTGGAGFIGSNFVLQWIEAGGRAVNLDLQTYAG
                     NPENLASIEDNPSHTLVRGDICDASLVSSLLREHKPRAIVHFAAESHVDRSIASPGEF
                     IRTNVEGTFRLLQESRVYWSELPENERAGFRFLHVSTDEVYGTLGMDDPAFSESTPYA
                     PNSPYAASKAASDHLVRAYFHTYGLPTVTTNCSNNYGPFQFPEKLIPLIILNALEGKP
                     LPVYGDGQNVRDWLFVGDHCTAIRTVLAKGKPGETYNVGGNSERANLDVVTEICNLLD
                     ELRPNPALGARTNLIRFVADRPGHDRRYAIDARKIQRELGWTPTVTFEEGLRRTVQWY
                     LNNKTWIENVQTGAYREWIRGNYEARLAR"
     gene            10418..11314
                     /gene="rfbA"
                     /locus_tag="VHE33_01390"
     CDS             10418..11314
                     /gene="rfbA"
                     /locus_tag="VHE33_01390"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR01207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HVT96128.1"
                     /translation="MKGIILAGGSGTRLHPVTHVISKQLLPVYDKPMIYYPLSTLMLA
                     GIRDVLLISTPQDTARFTELLGDGRKWGINISYAVQPSPDGLAQAFLIGREFIGQSPS
                     ALVLGDNIFYGQSFSSQLQSAAALKSGALVFAYRVHDPERYGVVEFDPAGHAVSIEEK
                     PANPRSHYAVTGLYFYDNDVVSLASSLKPSARGELEITDLNRLYLEAGKLKVVVMSRG
                     MAWLDTGTHESLFDAGSFVQTIQQRQGLKIACPEEIAYRLGYIGNEDLEALAGPIRKS
                     SYGQYLLELCREPQVAAEESAD"
     gene            11957..>15388
                     /locus_tag="VHE33_01395"
     CDS             11957..>15388
                     /locus_tag="VHE33_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96129.1"
                     /translation="MNCRLGSQNRSVSVRSLQPLDHRSNAWTIRVLALLALLFGTVAA
                     VKVHAQTAVYNGGITSTLNSNFLLPMSVTEDSSGNLFVADNDYNNATATVYKMTRTGP
                     GTYGAPTALPGPAGGYLCPGAGSVNQWEPNPCIRGLALDFYGNLWVADFNGGSGGQMY
                     ELVNNSGTFATPVAVGTGWVAPWGVSADPAGNIFVSDYGAETVLEITKASIGLGTPTV
                     TTIVNSGIVAQPRGLAVDASDNVYVIDGNVSHVMEIAAPAYASASPVNNYSFQGPGDL
                     AIDASGNLWVTEFQMQNVRELTFGSNYQTIFAWGSGLNGPVSVWPDKTDGTIRLVDAF
                     NHAIKKIMLQPAVNLGSTAVGSTSAAQTLTFTFTGYANATINAPVVLTQGITGKDFAD
                     ASTGTCTTTNGAGHPYIPASSCTVSVKFSPLAPGLRMGAVELLDTGGNLLAEALLYGT
                     GTGPQLTFSGPGQTVTKLATLTAPGIPQKIAVDASGNVYVADSYISKIFKIPAGGGTP
                     VQVASGFSGTPYGFTVDGAGNLFIGDTDHYRIVEVLAPGYSTINYVSPVLSGPPVGLA
                     VDTSDNIYVAEAAGGGVAAVQEVTAASSYSHVQTLSTSFLNPFGIAVDASGNVFVADK
                     GAASITEMVGGSSSNTHSYGSFSSPVDVAVDAAGNLYVADNGGGTITKLTKSSNYATS
                     VTLASSLSTPAGVALDGMGNVYYSADGSDNSVYELDRVHAPALAFLSSAYQTLSSDSP
                     RAVTVSNTGSAGSNLTFSGLVATTTSGGANSFTLSTGISDCTASSTLTSGNSDESCDV
                     GINFVPQSVGAIAGKVTLTDNATGSPQVVNVSGTGTKATPTITWASPSPIGYGTTLAG
                     VLTAAAKNGSNTVAGNYAYTEQVGAGSPSAVTASTVLAVGTYTLTVNFTPSDTTDFNT
                     PAAVQVSLTVNKATPTITWPTASAITYGQTLASSTLTGGSALNGATAVAGSFAFTAPT
                     TAPNAGTYAASVTFTPTDTTDYNPATSTVNVQVNLATPTITWPTAGAITYGQTLASST
                     LTGGSAKNGTTTVAGSFAFTAPTTVPNAGAYSASVTFSPTDTANYNPVTNTSVSVQVN
                     KATPTVPTPPTASNITFGQTLASSTLTGGVAKNGTTTVAGAFAFTTPTTAPPIGTN"
BASE COUNT         3167 a         4318 c         4635 g         3268 t
ORIGIN      
        1 cggatcgaga ttcggccgca ctccggccag ggagaggaag tcgacggttt ccatccgaag
       61 ggcggcgcgg ttcttcaccg tcttgacctg agcgctccgg ccgatcagct gtttcgcagt
      121 tttcacaagg actgcatcca gcgcaaggtt cgccgtgccg agcgcgagaa cctcgcgtac
      181 gaggagggaa gctccgaggc gcagatcctg gatttctacc gcctgatgac gatgacgcgc
      241 cggcgtcacg gactgccgcc tcagccactg cagtggttcc gggaactggt ggccgcattc
      301 ggaccgaacc tgaaaatccg gatcgcgcgc aaggacggtg ccgcggtggc gagcatcatc
      361 acgctgtctc accggcaaac gatggtctac aaatacggct gcagcgatgc ctcagcgaac
      421 aagtacgggg gcacgccgat gctgttctgg cagaccattc aggatgccaa aagcaacggc
      481 cagaccgaac tggatatggg acgctccgac ctcgaggacc cgggtctgag tgtttttaag
      541 gagcactggg gaagcatccc cacgcccctg acgtactgga agtttcccgc agaagctggg
      601 agaacgaggc cccagtggca aaaatcactg gccaaccgcg tgattgagtc cagtccggat
      661 cgtctgctgc aacttgcggg gaatgttctc taccggcaca tgggctgaag ctattatggc
      721 cggaaaaata acgcttcctt cacctgaccg ctcctgattc ctatgcttac cagactgtac
      781 tatcttgtaa aaccaattct gccctggcgt cttcgtgtgt cgatgcgtcg ctggcgtgcc
      841 gagcgccggc gccggcaata tgcggatgtg tggcccatcg atcccggatc cgatgcggtt
      901 cctccgcgct ggccgggctg gccggacggc aaacgtttcg ggctggtgct gacccacgac
      961 gtggaaggga aaaagggcta cgaccgcgtt ccgcgcctgg tggaactgac gcagaagtac
     1021 ggttttcgag ccagcttcaa tttcgttccg cacggcgaat atgaggtgac gccggcgatg
     1081 ctgcagctgc tggaaaacgc gggcttcgag gccggcgttc acggactgca gcatgacgga
     1141 aagctgtacc agtctccgaa gaaattcgcg gccaaggcgg cgcagattcg ggaaatcctg
     1201 caccagtggg gcgcggtggg tttccgctcg ccgctcatgc agcaccgtct ggggtggatg
     1261 cacgagctgg gatgcgagta cgactgttcg accttcgatg tcgatccgtt tgaaccggag
     1321 tccgatggga tgggcacgat ctttcctttc tgggtacagg ctccgggtag tgaaggtttt
     1381 gtggagttgc cctacacgct cgttcaggac ttcaaccttt tcaaggtcct gggagaatcg
     1441 agcatcgata tctggaaaaa gaaactggac tgggttgccg aacatggagg catggctctt
     1501 ttgaatacac atcctgacta catgacgatg gacgggtcac cggatcggga cgagtatccc
     1561 gtcgctcgtt acgaagaatt tctgcgctac gcgcgcgaca agcatggcga ccagtgctgg
     1621 cacgcactgc ctcgggaggt ctcccgccac tattgcgata acgtgccggc tgaatcccgc
     1681 aatacgcgcc gccggatctg catggtggcc tactcgggat atgaaaccga tggccggatt
     1741 cgccgctatg ccgaagccct ggctcggcgc ggagacatgg tccacgtaat tgcgctcgac
     1801 gggctcagca gcgggaagaa agtcaccacg ctgaacgggg tcacgatcta tcacgttcag
     1861 cgccgcgatc ataacgaatc gggccagtgg agctatgttt tccggatggt gcggttcctg
     1921 ttccggtcag ctgcggttct cacccggctg cacgcgcgca accgctacga cgtggtgcat
     1981 atccacaaca tgccggattt cctggttttt gcggcctggt accccaagat gaccggcgcg
     2041 cggctgatcc tcgatattca cgatgttgtt ccggaacttt ttgccaacaa gttcgcaaca
     2101 ggattgaaga aggtctatgt tgccggcctg cgtgtcgtgg agaagttgag ctgtcacttc
     2161 gtcgaccacg tgattatctc caatcacctg tgggtagaga agctgctggg gcgctccgcc
     2221 cgccccggtc aatgctcggt ggtggtcaat cttcccgacc cggaagtgtt ttaccgccgg
     2281 cagcgcaccc gccaggatga ccggtatatc gtgattttcc cgggtacgtt ccagtggcat
     2341 cagggcctgg atatcggtat cagagccttc gccgaattaa ggaagaggat gccgaacgcc
     2401 gaatttcatc tgtacggcgc gcagcacgaa aagctcatga actggctcaa gggcattgcg
     2461 caggagcttg ggctggaaga ctcggtgaag ttctgcggga ttgcggcact ggacaggatt
     2521 cccgagatca tcgccaatgc agacctcggc gtcgtgccga agcgcgcgga ctcctttggc
     2581 aatgaggcgt acagcaccaa gatcatggaa ttcatgtcgc agggaattcc ggtcgtggta
     2641 tcgcggacca aaatcgacag ctattacttc gatgacgaga cggtccggtt cttcccttcg
     2701 ggagacagtg cggccatggc tgaagcgatg tacgaggttg cacaaaatcc gacgctgcgc
     2761 gattcgctgg tgcggcgcgg ccttgaatac gtagagctgc acggctggga tcggaccaag
     2821 cagcagtatt tcgatctgat cgacgggctc tctactcaaa aatttgatgc atatgacctg
     2881 aagcggggcg taactcccgt agtcgaaggc tgaaaacttt ggattccatt acccacattt
     2941 ctgcaacaaa aatgggcctc gatcgcgctg tgcacgctgc gtcatctctg gatttgatgt
     3001 cttccgggac gacgatcgtg gaggtggatc cgtggaagga tccacgttgg gagaattttg
     3061 tcgtccggca tccggacgcc acgatttatc accatccagc gtggctcaag gtgctggagc
     3121 gcgagtatcg gcagcgatcg ctttttctgg cctgccagga tcgcgacgga acgatgcagg
     3181 gagtgtttcc gttgctctac acgcgcggac ttcctttcag ccgcggtccg ctgaccggac
     3241 caaggctttc gtcattgccg cgcactccgc tggccgggcc acttgctgtc gatcatgcgg
     3301 ttcgatgcgc actggtacgc gaagctttgc agcgcgcaac ccagcattcc gttcgtctgc
     3361 agatcaagct ggagacggaa cagctgaatg gcggcattga gggaatcgtc agaaaggcat
     3421 ggcgcgagag ctatatcgtt cctcttgccg gaaagcctgg ccagccctac cacattcccg
     3481 ggagccagaa tcgctccagc atcaaaaggt cgatcaacaa agccattgcg aacggcgtac
     3541 gaacgcgccc cgcggagaca gaagacgatc tgcgcgtgtg gtaccgtctc tatctcgaga
     3601 cgatgcgccg gaactttgtg ccggcacggc cctaccggtt cttccttggg ctgtgggaac
     3661 tgatgcgagg gagtgggatg atgcgtttgc ttctggctga acagcaaacg ccggaaggcg
     3721 ctcgtatcat cggcggtcac ttattctttg cgtttggctc gaccgtgact tatgcatttg
     3781 gcgcatcgcg ctcggcggat ttcggagttc gtcccaacga cattatcatg ggccaggcca
     3841 tcaacgacgc gagtgaagcc ggctacaagt ttgtggatct gggcgaggtg ccggatggcg
     3901 ataacgatct ggcccgattc aagagtaagt ggggagcgga gccggtgcgt ttgtatcgct
     3961 attactttcc cgactttccg gactcagacc actcgtcgga caaagcttac ggtaacgcat
     4021 tcatattcgc caggaaggtc tggaatcatt tgccgctggg actgacgtcg tggctgggag
     4081 accggattta cacttacctt tgagcgcgtg gggaagttgc ccaccgatcc ggagggaaat
     4141 gcatcgctga aaggaaaccg ccagtcctca tcgtccgcgg agaatatgga agtgcaatcg
     4201 tcgcctgaaa aagctccgga aatcgagagc ggcgccaggc cgctttcggc acaaccggcg
     4261 caccggttta cgcgacgttc gttcctgacg cttgcggctt cctctcttgg cgggcttgcg
     4321 gtctattccg gagaaattgc acgtcaccac ttgacggtcg agaattatac ggtccgtctt
     4381 ccccgcctgc ccgattcgtt ccggggcatg cggatcgcgc agatctccga cattcactac
     4441 ttcgagtgga ccgagagctt ctacctccgg caggtggtgg accacgtcaa cgccctcaag
     4501 ccggacctgg ttgtgttgac cggagatttc gtgacctacg ggccggtgcg ctggccgagc
     4561 caggacgcgc acaagagatt cgcgctccgg cacatgccgg aatgcgcttc gatcctgagc
     4621 ggcctgcgct gcccacttcg ttatgcgacg ctcggcaacc acgacatgat ggtcggcggc
     4681 cgatatgtgt acggcgatct ggtgaaccag gggctgccgg ttctcaggaa cagcgctgta
     4741 ccgctggaga gggatggcca gcgtctttgg ctggcaggtt tgggcagcgc gtgcgcaaga
     4801 gatgcggatc cggagcgcgc gtttccggcc agttctgttc gcgaccggga agcgatgatc
     4861 gttctcgcgc acgaacccga cattttgccc gagattgcca aatacaatgc cgacctgatg
     4921 ctggccggcc acacccatgg cgggcagctg tgcattccct tcctgcggcc cgcctacctg
     4981 cctgagtacg ggaagaaata tgtgcggggc tggttccgca gcggttccac tcaactctac
     5041 gtgaatcgcg gcgtcggtac gatcggggtt ccgctgcgcg tgaactgccc gcccgagatc
     5101 actctgctga cgctggcctg aatcccgcag gcggtcagaa ttccaatccc accgagcaga
     5161 agtccagcaa ctcttcgagt taccttttgc cggaggtctg atgacctggg acagctaacg
     5221 attgggtcat ttttccggaa atgcgaggaa acgccagcca tcgacgatac aggacgagtg
     5281 caaaaaacgt aactctccaa ggaaatccga ttgtgaacca atgggggatt gttgggaatc
     5341 tggccccccg ggtagaacaa ttgtgcgggt gtagtttaac tggcagaatt ggggccgtcc
     5401 aggtctcagg taggggttcg actcccttcg cccgctccat attcgaatcg caccctccgg
     5461 ttcacgacac aggccggcgt tggttatcga ggtctcacgg aaagaccgcg atcgaattcc
     5521 ccggttctgt acgcgagatt gggccagaat ggcaaattct gtcttggcga tttcctcaga
     5581 tttacacagg gcggctaagg ccgctgagtc gaaaggactc acgcattccg atgggcgaat
     5641 cccggtgctg gacggaatcc gaggtatcgc gatccttctc gtaatgacct tccatttctg
     5701 gattgttggg attgcgggta actcgacttc ttcatccatt tccacgggcg ctttttcctg
     5761 ggaacacatt tataactacg cagcgggaat gggctggctt ggtgtcgatt tattttttgt
     5821 actgtcgggt tttttgatta cgggtattct cttggactcg cgagagtctc cacgctactt
     5881 ccgggtgttt tatggccgga ggacggttcg aatctttccg ctgtattacg ccgctttaac
     5941 cgtcttcttt ctccttggcc cgtttgtgat ggcccatgtc tatcgtcccg cactggcaga
     6001 gatgcagagc ggcggaacgg taaaactgtt tgcgtggacc tatcttctga actggtacga
     6061 aggcttcagg ggatggagcg cggtggcaca tccgctccag catttctggt cgctgtccgt
     6121 tgaagagcag ttctatctca tttggccatt cctggtttta aagctggcgc ggcggcggct
     6181 gatgggcgtg tgcgtcggcc tgatgggggc tgcacttact ctgcgagcca tcctgtactg
     6241 gctgcatttg ccgttcgcag cttacctgtg gacgatttgc cgggcggatt cgctcgcgat
     6301 gggcgctatc gtggcgttgg ctgcccgcga tgccggcgat tgggacctgt ttctgaagtg
     6361 ggcgcgacgc ctggcggtgc cattactcgc cgctctcgtg ctgggaagaa ttttcaatcc
     6421 ccggtgcacg gtcggtccgg gagacacacc cacacttttc atgaacacgt tcgagctgac
     6481 cctggccgga ctgttcttcg gggtgtggat tgggctggct gtagcatcgc ataacgggaa
     6541 tccggctcat cgttttctgg cctcgccctt tctgcggttc ttcggaaaat acagctattg
     6601 tctgtacatt tgtcacctgc ccatcatcgt ggtgctggca cgggcaggac tgaactcggc
     6661 tcatctggcc ggcaagctgc acagccagtt gctggcggtt ctcgcagtcg acggtatcgc
     6721 gctcgccgtt tcgactatga tcgcttatgc aagctggcac ctttacgaaa aacagtggct
     6781 caagctaaaa aatctccacg gcatgcaacg cgaacagttg ctcccggcac aattggcggt
     6841 acaagaccga tgatggattg caccgcttcc cgtacggctc ctgaaattca aatccagttc
     6901 attcggcagc gctgcgaatc tccctgcctg ttcagagggg aaggatattg aagtgtcaga
     6961 gccacttttc agcatcgtaa ttgcctgcta caaccacgag aggttcatac gggacgccgt
     7021 cgagtcggcg ctgcggcaga acgcgagcag ggaagtcatc gttgtcgatg atgcttccgc
     7081 ggacggatcg gccgaagtgg tccggtcgtt tggcgcctcg gtcaccttcg aaaggctttc
     7141 cgtcaatcgc ggcgcggctg cggcacgcaa tcatggggcc tcggtggcga aagggaagta
     7201 cctgatcttc ctcgatggcg acgatgcttt gacgccgtgg acgctgaaag tgtacggccg
     7261 agtcattgaa gagcgcggcg cgaagctcat tctaggccga tcctcacttt tttacggtga
     7321 ccttcctgcc accgcggagg ctccgggtaa aattcgcttc gtcgactatg cgacgttttt
     7381 cgacaaggac cgcccctggg tctataacac cagttccctg gttgttgaac gcgaagcgtt
     7441 tcagcaggcc ggcggatggt cggaggctat tttctatcag gacattcagg accttctgaa
     7501 taagctctgc attgcgggca aaaccagtat ggtgctggcg ccggacacgg tgttttacag
     7561 aatgcattcg acgaacgccg ttcgccaggt cacgcctttc atcgagggga tcaatacttt
     7621 gctggcaaaa gccagacgag ggatctatcc tggtgatcgc gccgtacaaa agaagcgcgc
     7681 ggtctggttt ggcggcctga tcttctattg ggcgaaagaa ggattacgga acggacatct
     7741 gcgtgacgga ctgaagttgc tgttcttcaa ctggtggatg gttctgcttg cggtcatacg
     7801 gcgggtgatt gcgcggatcg gcggtcgaag gccaatcgag gtattgacgc tggagcctaa
     7861 tttcacggag aattctgtcg gttcggcgca tgcgcgcacc gtcacggaac atccctgaag
     7921 gacaggtata cacccgcgaa tcagagctca gtcgaagcgc gggcatcata agggagaaag
     7981 catgaatatc ggaattattg gctgcgggtt taccgcggat cattatttgt tctgtcttca
     8041 gtggcatccg gagttgaaag tcgtcggagc aaccgaccgg gatccggagc gggcatcgaa
     8101 attctgcaat ctccacaagg tgaagcttta tccgacgctg gaggacatgt tgtcggacgg
     8161 cagcatcgac atggtcctga acctgacgaa ttccagcagt cattacgaaa cctccaaagc
     8221 ctgtctgaat gccggcaaac atctgttcac tgagaaacct ctcgcaacgg atttttccca
     8281 ggcgaaggaa ctcgtagaac tcgctgacgc caagggcgtc tatttttccg ctgccccgtg
     8341 taacttgctg ggtgagactg cgcagactct gtggaaagct ctgcgcaaca aggaaattgg
     8401 ggatgttcgc ctggtcctgg cggaactgga tgacggtccc ttccatctgg cggcgcctca
     8461 cacatggcaa tcggaatccg gcgcgcccta cgactatcgc gaagagttca agatgggcgt
     8521 gacggtagaa cattcggcct attacctggg cctgtttgcc gcgttctttg gaccagccag
     8581 gacggttact ccattctcgg cagtgctgtg gcccgaacga agaatctcgt ctgacgagac
     8641 gctgcagctg acgacgccgg acttttcagt tgcctgtatc acgtttgagt cgggcgtggt
     8701 ggcacggttg acgtgcagcc tggtggcgcc gttcaaccac gtcctgaaaa tcacgggcga
     8761 tacgggcgtg atcacggtga acgaagtgtg gaactacacg gcaaaggtgt atgtcgataa
     8821 gtacacttcg atgagatatc gggcggaacg gtactcgatc accaaggagc atccggtcat
     8881 tgcgaacatg gttggaaacc acaaggttta tccgccggtg agaaaagcga acctgaaaaa
     8941 gcgctatgcc cggtaccaca tggattatgg gcggggtatc gccgatctgg cgctggccat
     9001 ccagcagcag cggccgcctc ggattccagc ggacttttgt ctgcacctta cggaactggg
     9061 cctggcgatc cagaaagcag agccgggacc ttatcaggtg aagactacct tcaagccatt
     9121 acagccgctg gatgaagccg gcctgaacga gctaatcccg gccaagtggt aggagcggaa
     9181 gctggcgcga cggcgttctt ctgacgaccg tcgcgcatgc aacgaaagaa tccaacttag
     9241 ggctcacgat gtgtctctgg tggcaagcgc tgccggcgtc cggggatgcc cggataacaa
     9301 aagatctgga tcgaagagga ctgatagcta tgaacgccac tgatgccaac ggaaccatcc
     9361 ttgtaactgg aggcgctgga ttcattggtt ccaattttgt gctgcagtgg attgaggcgg
     9421 gggggcgcgc agtcaacctt gatcttcaaa cctatgccgg caatcctgag aatctcgcgt
     9481 caatagagga caacccgagc catactctgg tgcggggaga catctgcgac gcctcactgg
     9541 tgagctctct gcttcgggaa cacaagcccc gagcgatcgt acacttcgcg gcagagagtc
     9601 acgtcgatcg atccattgcc tcgccgggcg agttcattcg aacgaatgtg gaaggcactt
     9661 tcaggctgct gcaggaatcc agggtgtact ggtcggagct tccggagaac gagcgggccg
     9721 ggtttcgctt cctgcacgtc tcgaccgacg aagtgtatgg aacgctgggc atggatgatc
     9781 ctgctttcag cgagagcacg ccgtatgcgc ccaacagtcc ctatgcagca tcgaaggcgg
     9841 catccgatca tctggtgcgg gcctactttc atacctatgg tctgccgacg gtaaccacga
     9901 attgctcgaa taactacgga ccttttcagt ttccggagaa actcattccg cttattattc
     9961 tgaacgcttt ggaggggaag cctcttccgg tttatggtga cggtcagaat gtgcgggact
    10021 ggctgtttgt tggcgatcat tgcacggcga tcaggacggt gctggcgaaa ggaaagcccg
    10081 gagagaccta caatgtcggc gggaacagcg aacgagccaa tctggacgtt gtgaccgaga
    10141 tctgcaacct gctcgacgaa ctgcgcccta atcccgcttt gggggcgcga acgaatctga
    10201 ttcgatttgt cgcggaccgg cctggccacg atcgccggta tgcgattgat gcgcgcaaga
    10261 ttcagcgcga actggggtgg acgcccactg taacgttcga agaaggatta cggcggaccg
    10321 ttcagtggta cctcaacaac aagacgtgga ttgagaatgt ccagaccggc gcctatcggg
    10381 aatggatccg gggcaactac gaggcgaggt tggcgcgatg aaaggaatta tcctggccgg
    10441 aggttccggc acccgcctcc atccggttac acatgtcatt tcgaagcagc ttcttcctgt
    10501 ctacgacaag ccgatgattt actacccgct gagcactttg atgctcgcgg ggattcgcga
    10561 cgtccttctg atctcaactc cgcaggatac ggcgcgcttt actgaattgt taggagatgg
    10621 gcggaagtgg ggcatcaata tcagctatgc tgttcagccc agccccgacg gtctcgctca
    10681 ggcatttctg attgggcgtg agtttattgg tcagtctccg agcgcgctgg tgttagggga
    10741 caacatcttc tacgggcagt cgttctcctc gcaactccaa agcgcggcgg cattgaagag
    10801 cggcgctctg gtgtttgcgt accgtgtgca tgatccggag cgctacggcg tggtggagtt
    10861 cgatccggcc gggcatgcag tgagcattga agagaaaccg gcaaacccac gatcgcacta
    10921 tgcggtcacc ggtctctatt tttacgacaa tgacgttgtg tcgctggcgt cgtcgctgaa
    10981 gccatctgca cgcggagagc tggaaatcac cgacctaaac cggctgtatc tggaagcggg
    11041 aaagctgaaa gtggtggtga tgagccgtgg tatggcgtgg ctggatacgg gcacgcacga
    11101 gtcgctcttt gatgcaggat cgtttgtgca gacgatccag cagcggcagg ggctgaagat
    11161 tgcctgcccc gaagagattg cctatcgtct tgggtatatc ggcaacgaag acctcgaagc
    11221 attggcgggg ccgattcgca agagctcgta cggccaatac ctgctcgagc tttgccggga
    11281 accgcaagtg gccgccgaag agagcgcgga ctagcgatcc cgggagaagc cctgcgacct
    11341 gcaccgttca atcaagcaaa aagcggacac ttagccaaaa atcctggatt gtgccgtact
    11401 ccaggtaagt cgtggtgtct gcgaaggggc actcagcggg cccgctgacc cattcggcta
    11461 cagcgcgtcc tgggtagact gccggagttt ccgtggttgc cgctcagcgg taactgcctc
    11521 ttacattacc gacggaccat gtcaattcaa cgaaggtggc gcatgctgcc gccgggcagg
    11581 ttcttaacca acggacgcag ggaacgcgcg gacggctcca agtgattggg tagctgcggg
    11641 aaacacggca aaaatgtgcc gtggttagag tggagcaggt attcgatagt ggaaaacagg
    11701 atgttgtaaa cctcgtgaag gccccggcca gagcccacga gaggtacctg accgggtaaa
    11761 ccaggtaatc caatagtttc tatttccggt ccaacctggg gattgctttc atcagcagga
    11821 ccgatagaat ttgcccacga tcatggcaag gagcagctgt ttgttttgat cgccggtcga
    11881 tcgaataccc gccagttgga ttcagatcgc cgtcttgcct gatccgcatt tgcctccagt
    11941 acaggagaag ttttgaatga attgccgcct tgggtcccag aaccgtagtg tctctgtacg
    12001 tagccttcaa ccgctcgatc atagaagcaa tgcgtggacg attcgcgttc tggcgttgct
    12061 tgcgttgctg tttggtacgg tcgccgcagt taaggttcat gcgcagacgg cggtttacaa
    12121 cggcggcatc acgagcacac ttaactcgaa tttcctgctc ccgatgagtg tcacggaaga
    12181 ttcgagcggc aatttgtttg ttgccgacaa tgactacaac aacgcgaccg cgacggtcta
    12241 taagatgacg cgcaccggtc cgggtacgta cggagcgccg acagctcttc cggggccggc
    12301 gggcggatat ctctgccctg gagcgggctc ggtgaatcag tgggaaccga acccctgcat
    12361 ccgtggtctg gccctcgact tctatggcaa cctgtgggtc gccgacttca acggcggttc
    12421 gggcggacag atgtacgagc tggtcaataa ctccgggacc ttcgcgacgc cggtagcggt
    12481 tggaaccgga tgggtcgctc cctggggcgt cagcgccgat cccgcgggaa acatttttgt
    12541 gagcgactac ggcgccgaaa ccgtattgga aatcaccaaa gcctcgattg ggctgggaac
    12601 tccgaccgtc acgacgatcg tgaactccgg catagtcgcg caaccgcgcg gcctggcggt
    12661 ggacgccagt gacaacgttt atgtcatcga cgggaatgta agtcatgtga tggaaattgc
    12721 cgctcctgca tatgcctctg cgagcccggt gaataattac agtttccagg gaccgggcga
    12781 tctggccatc gacgccagcg gcaatctctg ggtaactgag ttccagatgc agaacgttcg
    12841 cgaactcacg tttggaagca actatcagac gatctttgcc tggggcagcg gactgaatgg
    12901 cccggtttcg gtgtggcccg ataagacgga cggcacgatt cgccttgtag acgcgttcaa
    12961 ccacgcgatc aaaaagatca tgctccagcc agccgtcaat ctgggaagca cggcggttgg
    13021 ttcgacttcg gcggcacaga ccctgacgtt cacgtttacc ggctacgcca acgccacgat
    13081 caacgcgcct gtggttctta ctcagggaat tacgggcaag gattttgccg atgcatcgac
    13141 gggaacctgc acaaccacca atggcgcggg tcatccttat atccccgcct ccagctgcac
    13201 cgtgagcgtg aaattcagcc cgttggctcc cggccttcgc atgggtgcgg tggaacttct
    13261 ggacaccggt gggaacttgc tggcggaagc gcttctctac gggacgggca ccgggcctca
    13321 gcttactttc tccggcccag gtcaaaccgt cacgaagctg gccacgttaa cggctcccgg
    13381 cattccgcaa aaaatcgccg tggatgcgag cggcaacgtg tatgtcgccg attcttatat
    13441 cagcaagatc ttcaaaatac cggcgggagg cggcactccg gttcaggtgg cctccggatt
    13501 ctccggtacg ccttacggtt tcacagtgga cggagcaggg aaccttttca tcggcgatac
    13561 cgaccattac aggattgtcg aggttctggc gccaggttac agcaccatca actatgtgtc
    13621 gccggtgctg tcgggacctc cagtgggcct cgcggtcgat accagcgata acatctatgt
    13681 tgccgaggcc gcgggtggtg gcgtcgctgc tgtacaggaa gtgacggcag ccagcagtta
    13741 ttcccacgtc cagactctca gcacatcgtt cctcaatcca ttcggaattg cggtcgatgc
    13801 cagcggcaac gttttcgttg cggacaaagg cgccgcctcc atcaccgaga tggtgggtgg
    13861 atcctccagc aacacgcaca gctacggttc gttcagcagt cctgtggacg ttgccgtgga
    13921 cgcggccggc aatctgtatg ttgccgataa cggcggcggc acgatcacga aattgacaaa
    13981 gagcagcaat tacgccacct cggtaactct ggccagcagt ctttcgactc cggccggagt
    14041 agctctggac ggcatgggaa atgtctatta ttcggccgac ggcagcgata actcggtgta
    14101 cgaactcgat cgcgtgcatg cgccggcgct ggctttcctg tcgagtgctt atcagacgct
    14161 gagcagtgac agcccgcggg cggtcacggt ctctaacacc ggcagcgccg gatcgaatct
    14221 gacgttcagc gggctcgttg ccaccaccac atcgggtggc gcgaacagct ttaccctgag
    14281 caccgggatc agcgattgca ccgcgtcttc cactctcacc agcggaaaca gcgacgaaag
    14341 ctgcgatgtc ggaatcaact tcgtaccgca gtcggttggc gcgattgccg gcaaggtgac
    14401 ccttacggat aatgcaacgg ggtcgccgca ggtggtcaac gtcagcggca cgggcacgaa
    14461 agccacaccg accatcacgt gggccagtcc gtcgccgatc ggctacggca caacgctcgc
    14521 cggcgtgctg actgcagcag cgaaaaacgg gagcaatacc gtggccggga actatgccta
    14581 taccgaacag gtcggtgcag gctccccgtc agccgtgacc gcttcgacgg ttctcgcggt
    14641 gggcacgtat acgctcacgg tgaactttac tccgagcgac acgactgact tcaatacgcc
    14701 ggctgctgtg caggtttcgc tgaccgtcaa caaagcgaca cccaccatca cgtggccgac
    14761 ggccagtgcg atcacctatg ggcagacgct ggcatcctcc actttgacgg gtggaagcgc
    14821 gctgaacgga gcgaccgcgg ttgcgggcag ctttgccttc accgcgccaa cgacggctcc
    14881 caacgccggc acgtacgctg ccagcgtgac gttcactccg acggacacca cggattacaa
    14941 cccggcgacc agcactgtga acgtgcaggt gaatttggca acgccgacca ttacctggcc
    15001 gactgccggc gcgatcacct acggtcagac gctcgcctcg tcgacgctga cgggcggcag
    15061 tgcgaaaaat ggcacgacaa cggttgccgg cagcttcgcc ttcacggcac cgacgacggt
    15121 tcccaacgcc ggggcttact cggcgagcgt cacattcagc cccacggaca cggcgaacta
    15181 taacccggtg acgaacacga gcgtcagcgt tcaggtcaat aaggccacgc cgaccgtacc
    15241 tacgccgccg actgccagca acatcacctt tggccagaca ttggcctcat ccaccctgac
    15301 cggtggcgtt gccaagaacg gcacgacgac tgtagccggc gcgttcgctt tcacgacgcc
    15361 gaccacagca ccgccgatcg gaaccaac
//
LOCUS       DASZYN010000049         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_322406, whole genome shotgun sequence.
ACCESSION   DASZYN010000049 DASZYN010000000
VERSION     DASZYN010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322406"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VHE33_01400"
     CDS             complement(<1..188)
                     /locus_tag="VHE33_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96130.1"
                     /translation="MYCSACGQPIDAYSPYCPRCGRQVIPIATATPPPWAWTRVHRHV
                     HTLGILWIAYAGLTLMSWL"
     gene            354..1202
                     /gene="accD"
                     /locus_tag="VHE33_01405"
     CDS             354..1202
                     /gene="accD"
                     /locus_tag="VHE33_01405"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HVT96131.1"
                     /translation="MSWFRREDNKIESGENRVRTEGLWIKCEGCNQPIWKADLEANLQ
                     VCPKCGRHGRISAQARINSLLDPGYTWLNGDLKSTDPLNFVDLKPYKERLKKARSQTG
                     LYDAIVNVQGQMGPHNVVLSVMEYSFIGGSMGAVVGETIARAIDKAYEDRQPLITVAA
                     SGGARMMEGIVSLMQLAKVSTALTRLDEAGIPFISVLTDPTTGGVTASFAMLGDLNIA
                     EPGALIGFAGPRVIEQTIRQKLPEGFQRSEFLLQHGFLDAIVGRHEMKAYLIRALDFM
                     GVPKAA"
BASE COUNT          318 a          445 c          617 g          328 t
ORIGIN      
        1 agccagctca tcagggtgag gccggcgtag gcgatccaga ggatgccgag ggtgtggaca
       61 tggcgatgga cgcgggtcca ggcccagggt ggaggcgtag ccgtcgcaat gggaatgacc
      121 tggcgcccgc atcgcggaca gtagggcgaa tacgcatcga tcggctggcc acatgcgctg
      181 cagtacatcg tgaggacctc cccgcctggc gacaatagag ggtacgcagg cggacggcgt
      241 ccggttccgg acaaaaagct gcgatcggcg tcccgcgttt ggtgggccgg acgcgtcgcg
      301 cctggcctga taatcttgtg ttcggcagat tgctgctgct cggggagttg cggatgtcct
      361 ggttcaggcg ggaagacaac aagatcgagt cgggcgagaa ccgggtgcgc accgaggggc
      421 tgtggatcaa gtgcgagggg tgcaaccagc ccatctggaa ggcggacctc gaggccaatc
      481 tgcaggtttg tccgaagtgc gggcggcacg ggcggatcag cgcccaggcg cggatcaaca
      541 gcctgctgga ccccggctac acgtggctga acggcgatct gaagtcgacc gatccgctga
      601 actttgtgga cctgaaaccc tacaaggagc ggctgaagaa ggcgcggagc cagacggggc
      661 tgtacgacgc catcgtgaac gtgcaggggc agatgggtcc gcataacgtg gtgctgagcg
      721 tgatggagta cagctttatc ggcggcagca tgggcgcggt ggtgggggag acgatcgcgc
      781 gggcgatcga caaggcctac gaggaccggc agccgctgat cacggtggcg gcttcgggcg
      841 gggcgcggat gatggaaggc atcgtgagcc tgatgcagct ggcgaaggtg tcgacggcgc
      901 tgacgcggct ggacgaggcg gggatcccgt ttatctcggt gttaactgat ccgacaacag
      961 gcggcgtgac ggcgagcttt gccatgctgg gcgacctgaa cattgccgag ccgggggcgc
     1021 tgatcgggtt tgcgggaccg agggtcatcg agcagaccat ccggcagaag ctgccggagg
     1081 gcttccagcg gtcggagttc ctgctgcagc acgggttcct ggacgcgatt gtcgggcggc
     1141 acgagatgaa ggcatacctg atccgggcgc tggactttat gggcgtgccg aaggcggcct
     1201 gaaggacggg gcgcggagct gagggtacag ggcgcagggc ccagaacgcc gattcgtcgc
     1261 ggatttccct tgggcaagtt gcatgttgtt gattctgtgc gacttctcct tgttgacaag
     1321 cttgtcaact ttttctaatt cattctaaag aacttattta cggcccttgg gttcctgacg
     1381 cggccgtctg ataccaattg cggccgtttg tgtaacacga gacggccgtg tgtgttacaa
     1441 acttggccgt ttgataacaa actcccgtcc gtttcatcgg gtctcggcgg gctttttggc
     1501 ttcgtcgcat cggctcgaga tgggctccgg gcgatctgcc cgaggtccag acgaacgaaa
     1561 acggccgcca ctcggcggcc tatctgagca atcccctcta ttttcagaat accaaaccgg
     1621 gcgggaaatc gtgccaaact ttttggggtg atttggcgtg gaagtcgtgt ggaatcacag
     1681 gtcattacct tggttttggg ggtggggg
//
LOCUS       DASZYN010000050         4672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_130867, whole genome shotgun sequence.
ACCESSION   DASZYN010000050 DASZYN010000000
VERSION     DASZYN010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130867"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VHE33_01410"
     CDS             complement(<1..323)
                     /locus_tag="VHE33_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96132.1"
                     /translation="MSASMYIVVEGEDPGFDIFVNGHALARNEDALEKLALRLGVKPL
                     LEFFSADENSMALLLEEGAGDPEWARTLPPPQWFSPEDGLITICTLHDFLKEAPTALG
                     ADTTRV"
     gene            complement(557..3118)
                     /gene="pnp"
                     /locus_tag="VHE33_01415"
     CDS             complement(557..3118)
                     /gene="pnp"
                     /locus_tag="VHE33_01415"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HVT96133.1"
                     /translation="MKQEVSVELAGGKRLHFETGRMAKQASGAALVTSGDTVVLATAV
                     ASPDPREGIDFFPLTVDYREYAYAGGRIPGGFIKREGRPSEREILTSRQIDRPIRPLF
                     PEGFRNETQIIALVFSADKENDPDIIGINAAGAALALSDIPFGAIIGAVRVGQINGEF
                     VVNPSYTERKESKVNITVVATKDGIVMIESGAAEASEETVIDAIEFGHAEIKKIVAAI
                     EDLVSRAGKQKRAVTAPEFDTSYFEALSAKVGSRLKDALDTKAHPKLESQTLVKQIQD
                     ELKAEIPADDKAGEAKKKLAHYFETLRERLFREQVTKERIRPDRRAFDEIRPITIEVG
                     VLPRTHGSALFTRGETQALVTATLGTTDDSQRLETFEGEQRKKFMLHYNFPPFSVGET
                     GRMTGVGRREVGHGALAERAISAVLPSEEESPYSLRIVSDILESNGSSSMASVCGASL
                     ALMDAGIPLKQSVAGIAMGLVKEGDDYAILTDIAGAEDHYGDMDFKVAGTRNGITALQ
                     MDIKIGGITGQIMREAMEQARRGRLFLLDTMEAVLNGPRTEKSKFAPQIRTLQIPTDK
                     IRDLIGPGGKTIRGIIEATQVKIDVDDTGKVNVASSDEEGLKKALAMINDLTAVPEVG
                     KVYLGKVVRLAEFGAFVEIFPGTDGLLHISEIAEHRVREVKDELREGDQVMVKVLGVE
                     GNRIKLSRKAVLREQRQKLGLPDPNQGSDMAASGEGSERPPRGERHDRGERHDRGERS
                     DRGERGERQERAPRPPRPAPVAPEERQPTSNASTIMIEGGEDFDDDDGPPLEDGEEIN
                     FNRADGVPVVVGPQGERRPGGPGGGASGGPGGQRRRRRRGRRPGGPGGSGGGNRG"
     gene            complement(3325..3591)
                     /gene="rpsO"
                     /locus_tag="VHE33_01420"
     CDS             complement(3325..3591)
                     /gene="rpsO"
                     /locus_tag="VHE33_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HVT96134.1"
                     /translation="MLAPEKKTEVIARYRTHDSDTGSPEVQIAVLSERIGDLTEHFKT
                     HAKDHASRRGLLMLVSKRRRLLDYLKTHDSERYRTVITKLGIRK"
     gene            complement(3895..>4672)
                     /locus_tag="VHE33_01425"
     CDS             complement(3895..>4672)
                     /locus_tag="VHE33_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96135.1"
                     /translation="LGWATQKKALRAMAYYDHTPLRMLPENRVDELCPDGVCPKLVFL
                     PSAQGLTEKAWQLLLGYVEKGGTLLLTGPVNRDEHWMEVDRTGPLGVKAEVMPLAVRG
                     SSLMLPEGKTFTVGFPADVQQSSVETMQFADGKTVEVIPHGSGKMLWASNPVELAEGY
                     EATDALYRWALDQAAVKTPFRETTPLSNAVLAFPTVLNDAELYSFSNESANHQPVDIV
                     DALTGVRIRFAMEPQRGAALLLNRQGKVLASYGGAALAAR"
BASE COUNT          815 a         1586 c         1274 g          997 t
ORIGIN      
        1 acgcgggtag tgtcggcgcc cagagctgtc ggcgcttcct tcagaaaatc atgcagggtg
       61 cagatcgtga tcaacccatc ttcaggtgaa aaccattgcg gaggaggcaa agtcctggcc
      121 cattccggat ctccggctcc ctcttcgagc aacagagcca tggaattctc atcggcggag
      181 aagaactcaa gcagcggttt cacacccagg cgaagagcca gtttctccag agcatcttcg
      241 ttgcgggcca gcgcgtggcc gttcacaaag atgtcgaagc cgggatcttc tccttcgaca
      301 acgatgtaca tggatgcgct cattggcgtc agtctacctg atgtgaggat gcccgacgag
      361 gcccgtggga tggttcgttg ccagatgagt aactaactcc catgatccct ctgcttttgg
      421 gccaatcgat tgatgacgcg caatttaacc aaaaagacgc gagaattttt gcggtcccgg
      481 ggaacccatt cctgctgcgc aaaaacagag ggccggatcg gctgatccgg ccctctgagc
      541 tgacaacgaa gcgttgttat ccgcggtttc cccctccgct cccgcctgga ccacccggac
      601 ggcgtccacg ccgccgacgg cgacgctggc cgccgggtcc accgcttgcg ccaccccccg
      661 gtcctcccgg acggcgctcg ccctgcggtc ccaccaccac cggaactcca tcggcgcggt
      721 tgaagttgat ttcttcacca tcttccagcg gcggcccatc atcgtcgtca aaatcctcgc
      781 cgccctcaat catgatggtg ctcgcattcg acgtcggctg ccgttcttca ggagcaaccg
      841 gcgcgggccg tggcggacgc ggcgcacgtt cctgacgctc gccccgctct cctcggtcgc
      901 tgcgctcgcc acgatcgtga cgctcgccgc gatcgtgccg ttctccgcgt ggaggccgct
      961 ccgatccttc acccgaagcc gccatgtcgc tgccctggtt cggatccggc aggcccagct
     1021 tctggcgctg ctcgcgcagc acagccttgc gcgacagctt gattcgattg ccctcaacgc
     1081 cgagaacctt caccatcacc tgatcgcctt cgcgcagttc gtctttcact tcacgcacgc
     1141 gatgttcagc gatttcgctg atgtgcagca gcccgtcggt gccggggaag atctccacga
     1201 acgccccaaa ctccgccagc ctcaccactt tgccaaggta gactttcccc acctccggca
     1261 ccgcggtcag gtcgttgatc atcgccagcg ccttcttcag gccttcctcg tcgctcgacg
     1321 ccacgttcac cttgcccgtg tcgtctacgt cgatcttcac ctgcgtcgct tcgatgatgc
     1381 cgcgaatcgt ctttccgccc ggcccgatca ggtcgcgaat cttatctgtc ggaatctgca
     1441 gggtgcggat ctgaggtgcg aacttcgatt tctccgtccg cggcccgttc agaaccgctt
     1501 ccatcgtgtc cagcaggaac agccggcctc tgcgcgcctg ctccatggcc tcgcgcataa
     1561 tctgtccggt gatcccgccg atcttgatgt ccatctggag ggcagtgatg ccgttccgcg
     1621 ttccggcgac tttgaagtcc atatcgccgt agtgatcttc agcgcccgca atgtcggtca
     1681 ggatggcgta atcatcgccc tctttcacca gtcccatcgc gatgccggcc accgactgtt
     1741 tcaacggaat gcccgcatcc atcagcgcca gactggcgcc gcaaaccgaa gccatcgacg
     1801 acgagccgtt cgactccagg atgtcggaca cgatacggag cgagtacggc gactcttctt
     1861 cactcggcag caccgcgctg atcgcgcgct ccgccagtgc gccgtgtcca acttcacgcc
     1921 ggcccacgcc cgtcatgcgc cccgtctcgc cgactgagaa cggagggaag ttgtaatgca
     1981 gcatgaactt tttccgctgc tcgccttcga aggtctccag ccgttgcgaa tcgtccgtgg
     2041 tgcccagtgt tgccgtcacc agtgcctgag tttcgccgcg ggtaaacagc gccgagccat
     2101 gcgtccgggg cagcacgccc acttcaatgg tgatgggacg aatctcgtcg aaggctctgc
     2161 ggtccggacg aatccgttct ttggtgactt gctcgcggaa cagccgctcg cgcagggtct
     2221 cgaagtagtg cgccagcttc ttcttcgctt cgccggcctt gtcgtcggcg ggtatctctg
     2281 ccttcaactc gtcctgaatc tgcttcacca gagtctggct ttcaagcttg ggatgcgcct
     2341 tggtatcgag cgcgtctttc agccggctgc ccactttggc gctcagcgct tcaaaatagg
     2401 aggtgtcgaa ttccggcgcc gtcaccgctc gcttctgttt gcctgcgcgc gagaccagat
     2461 cctcgatggc tgcgacgatc ttcttaatct cggcgtggcc aaactcgatc gcgtcgatca
     2521 ccgtttcttc gctcgcctcg gcggctccgc tctcgatcat cacgatgccg tcttttgttg
     2581 ctaccaccgt gatgttgacc ttgctctcct tacgctcggt atacgacggg ttgacgacga
     2641 actcgccgtt aatctggccg acgcgcactg cgccaataat cgcgccgaac ggaatgtccg
     2701 acaaagccag tgccgctccc gccgcattga ttccgatgat gtccggatcg ttttccttat
     2761 ccgctgagaa gaccagcgcg atgatctgcg tctcgttgcg gaaaccttca gggaacagcg
     2821 gacggatcgg gcgatcgatc tgacggctgg tcaaaatctc gcgctcgctc gggcgccctt
     2881 cgcgcttgat gaatccgcca gggatgcgtc caccggcata cgcgtactcg cgatagtcaa
     2941 cggtcaacgg gaagaagtcg atgccttccc gcggatcggg ggaggcgact gcggtcgcca
     3001 gcacaacggt gtcccctgaa gttaccaggg ctgcgcctga ggcctgtttc gccatgcggc
     3061 ccgtctcaaa gtgcagtcgc ttgccaccgg caagttcgac ggaaacttcc tgtttcatat
     3121 tttttcctcg tttcttaacc aataggcggg gtatccgcgg ctgggcgggc gatggggagc
     3181 cgggaaagag cggggattac ggattcaact cgctgcaccg agccctgtct gggatcaggc
     3241 tgtgccgccg ttatgggcgt cgccccaggg ctcgtgctct ctcggttctc gtcgctcgcg
     3301 cgccgaagac ctggcctgtg cgtgttattt ccgaatgccc agcttggtga tcacagtccg
     3361 gtaacgttcc gaatcgtggg tcttgagata gtccagcagg cggcggcgct tgctcaccag
     3421 catcagcagt ccccgccgtg aagcatgatc cttcgcgtgg gtcttgaaat gctcggtcag
     3481 gtcgccaata cgctcgctca gaaccgcaat ctgcacttca ggactcccgg tatcggaatc
     3541 gtgtgtgcga tagcgtgcga taacttcggt tttcttttcc ggtgccagca cctttgtgct
     3601 actcctctga tttctctacg gatacacttc ctaagtgtct gccccaagaa taacatggcg
     3661 ggctgccggg cgccaccagg gaggtgacgg tcgtgcccaa acaacggtgg cgtctcctcg
     3721 aatagctccg ccagaaaccc ctcccgatgg ttcatgagtc ctttcacgat ctgatacggc
     3781 gctgtctcct cgtattccat tgggcgaagc atcccgtcgt cgaacgagac gagctcgcct
     3841 gcggataatc ccggcaggat cggagagtaa gtcgaaatgc attcccgcaa cctgtcacct
     3901 ggcggcgaga gccgcgccgc cgtaactcgc caaaaccttc ccctgacgat tcagcaggag
     3961 cgccgctccc cgttgcggct ccatggcgaa ccggatacga actccggtca gggcatccac
     4021 gatgtccacc ggctggtggt tcgccgactc attgctgaag ctgtacaact cagcgtcgtt
     4081 cagcaccgtt ggaaatgcca acaccgcatt cgacagaggt gtagtctccc gaaacggtgt
     4141 cttcaccgct gcctgatcca gcgcccagcg gtacagcgcg tccgtcgctt cgtacccctc
     4201 tgcaagctcc accggattgg aggcccacag catcttcccg cttccgtgcg gaatcacctc
     4261 gacggttttg ccatctgcga attgcatcgt ctccaccgac gactgctgca catccgccgg
     4321 aaatcccacc gtgaacgtct tcccctccgg cagcatcagc gacgatccgc gcaccgccag
     4381 tggcatgacc tccgccttca cccccagcgg ccccgtccga tccacctcca tccagtgctc
     4441 gtcccgattc accgggcccg tcagcagcag cgttccgccc ttctccacat acccgagcag
     4501 cagctgccac gccttctcgg tcagcccctg tgccgacggc aaaaacacca gcttcggaca
     4561 aacaccatcc gggcacagtt catccacccg attctccggc aacatgcgta acggtgtgtg
     4621 atcgtaatac gccatcgccc gcagcgcctt cttctgcgtc gcccacccca gc
//
LOCUS       DASZYN010000051         6757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_709257, whole genome shotgun sequence.
ACCESSION   DASZYN010000051 DASZYN010000000
VERSION     DASZYN010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709257"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VHE33_01430"
     CDS             complement(<1..600)
                     /locus_tag="VHE33_01430"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVT96136.1"
                     /translation="MISSPTPILVRNGRLIDPASNLDAARDVLVRDGKVAAIETAGKI
                     KAAGAEIVDAKGLIVSPGLIDIHVHLREPGQGYKETIATGTAAAAAGGFTTVCAMPNT
                     IPVNDTPEITRWMRSAERGAAIRVLPIGAATIGSNGETLTNYAALKDAGAIAVTDDGR
                     PILGDAIMLEALRAATRVGLPVIQHAEDTRLTGGCSMNAG"
     gene            complement(597..1625)
                     /locus_tag="VHE33_01435"
     CDS             complement(597..1625)
                     /locus_tag="VHE33_01435"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF002032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HVT96137.1"
                     /translation="MALTRAAIRKPPQKPSAPLFFQQPIPPGSAISAADFAVADVRRI
                     QETADALEKENPFRRAERLAKRRVALLFYESSTRTRTSFELAAKALGADTTLVSSLSS
                     SIEKGESLKDTGITLKALGAECIILRSPYSGGPYLLARATGLPVLNAGDGMAEHPSQA
                     LLDLRTMLQFLGIPARRISEKVLRGVTVSIVGDILHSRVARSNALLLPRLGAKVILCG
                     PPALLPETAACMRPGLMIERDFDAALRASTIVMMLRIQAERLAGLSIDLDDYRIRYQA
                     NGDRIAAQAPKALIMHPGPIIRGMEITAEVADGPQSGIAQQVHNGVAVRMALLVRALG
                     TAKKGGRR"
     gene            complement(1653..2243)
                     /gene="pyrR"
                     /locus_tag="VHE33_01440"
     CDS             complement(1653..2243)
                     /gene="pyrR"
                     /locus_tag="VHE33_01440"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HVT96138.1"
                     /translation="MIAFTPMSELQATATPKLREKGRIMSASEIERTLVRLAHQIIEK
                     NNGAETIGLVGIKRRGVPIAQRLATLIGGIEKHQVDTGVLDISFYRDDLSTRDVRPVV
                     VPGSLGFDVNGRDVILIDDVLYTGRTIRAALDALFDHGRPRRVQLLVLIDRGHRELPI
                     EATFVGRSVPTSQREIIEVKLKEIDNDEQVLLVERV"
     gene            complement(2331..3005)
                     /locus_tag="VHE33_01445"
     CDS             complement(2331..3005)
                     /locus_tag="VHE33_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96139.1"
                     /translation="MELLCHRCGNTLHEGESFCPHCGAPQLVVDTSEPTGPQQPAQRL
                     GTDPSRVEWRVAIGYSLLLAVPVGLLSAFAGMSSVFVIAGGFGAIALYRRRTAALTDG
                     SLGWRVGAILGAAAASIATLADGLRLLVERYFLHHGAEIDGQFASVAQQLADQALKSN
                     SQAMQQAPQLVHNWANFWLSADGHAAIQLLTASMVSAGMILFAATGGAIAGRILSVRP
                     RTQRTM"
     gene            complement(3089..3703)
                     /locus_tag="VHE33_01450"
     CDS             complement(3089..3703)
                     /locus_tag="VHE33_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96140.1"
                     /translation="MRLRVRLLPEAFILGALLAAVGCHVSVDKGKNGEKKNVRIDTPL
                     GGLHVRSDQTTALDLGLPAYPGAQVSPDHEGDKSADVHFGFGQFQLRVRVVSYESSDD
                     RDKVVAFYKNAMGRFGDVLECQGNHPVGAHTITSEGLSCSEDSKVKVQGNGFDNNDEF
                     SLRAGSKHHQHIFAIKNGGPGTRFALVELQLPSGLDEHASKTSD"
     gene            4099..4665
                     /locus_tag="VHE33_01455"
     CDS             4099..4665
                     /locus_tag="VHE33_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96141.1"
                     /translation="MDRTRTIYRAAGTGLVALVTLAAGCNNKNKPTPENFMKTMNAYF
                     QDRNDCLFPQGHTWPYEVAPSPTAKDDKKQMDALTDAGLLTRLEDRSMNVSRYSLNGL
                     AGKRFAPRFCYGHREVTSIVSNTPPGPKNGFTETDVTYEYQMRDVPVWANSDAVKAAF
                     PQLGKSLGNTPTDQMTLATVGAGWQVPR"
     gene            complement(4675..4878)
                     /locus_tag="VHE33_01460"
     CDS             complement(4675..4878)
                     /locus_tag="VHE33_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96142.1"
                     /translation="MAKRKSPLISTVVLVVIFGSLGLMNLSGKDRFKSYRTVDVVQLL
                     GIGMCYGVALMGVGMLIRKSDPD"
     gene            complement(4969..5045)
                     /locus_tag="VHE33_01465"
     tRNA            complement(4969..5045)
                     /locus_tag="VHE33_01465"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5009..5011),aa:Pro,seq:ggg)
     gene            5155..>6757
                     /locus_tag="VHE33_01470"
     CDS             5155..>6757
                     /locus_tag="VHE33_01470"
                     /inference="COORDINATES: protein motif:HMM:NF028457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5117 domain-containing protein"
                     /protein_id="HVT96143.1"
                     /translation="MPRLRTSLLAVAFPLALIVSSTRSSPAAAQTADSKPPASIADKT
                     KFMVHMPGLLPLDWDAKAGKLYLEVPLDGNAGHTRSQDMIWTNSTPWSMGSSQLGGYG
                     MDRGQMAPGVIVRFERTGPKLLLVQSNLDFRSSSTDAAERAAVEESFPVSVVAGFTVD
                     AENSDGTVLVDATQFFLTDVHHVAEGLLQGKQGTFHVDPARSTFVLQHTKAFPKNTVV
                     EAELTFIPRPEDLQPVGKILREVSPDARAVTIRERQNFVELPPPGYTPRRFSPRAGYF
                     PMSYRDYDAPLSAPLTEQFILRHRLIKKDPNCTHACEAVAPIQYYVDRGAPEPLRSAL
                     VEGARWWDQAYQAAGWAPGTFKVDVLPQDADPMDVRYNIIQWVHRYTRGWSYGDAMAD
                     PRTGEIIKGNVTLGSLRARQDYLIAEALLAPYEKGKPPADEAHDPALQMALQRIRQLG
                     AHETGHTLGLAHNFAASAFPHSPDQTMSVMDYPHPWVTLGPNGVPDLTHAYGVGIGTW
                     DKVAIDYGYREFDRDGKPVEDAAALD"
BASE COUNT         1314 a         2204 c         2015 g         1224 t
ORIGIN      
        1 ccccgcattc atcgagcaac cgcccgtcag gcgcgtgtct tctgcgtgct ggatcaccgg
       61 cagccccaca cgcgtcgcgg cgcgcagggc ctcaagcatg atggcgtcgc caaggatcgg
      121 ccggccatcg tccgtgaccg caatggcgcc ggcatctttc agcgccgcat agttggtcag
      181 tgtttcgccg ttggacccga ttgtcgctgc cccgattggc aatacccgaa tcgcagcgcc
      241 tcgctccgcc gaccgcatcc agcgggtaat ctcgggcgtg tcattcaccg gaattgtgtt
      301 gggcatcgca cagacggtgg tgaacccgcc tgcggccgct gctgcggttc cggttgcgat
      361 ggtctccttg taaccttgtc caggctcgcg cagatgaacg tgaatatcga tcagccccgg
      421 actaacgatc agccccttcg catccacgat ctccgcgccg gcggccttga ttttccccgc
      481 ggtttcaatc gccgccacct tgccatcgcg gacaagaacg tcgcgcgcag cgtcaaggtt
      541 cgacgcggga tcgatcaggc gtccgtttcg aaccagaatc ggcgtcggcg atgagatcat
      601 cgacggccgc ccttcttcgc tgtgcccagc gcccgaacca gcagagccat ccggaccgcc
      661 acgccattgt gcacctgttg cgcgatgccc gactgcgggc catccgccac ttcggccgtg
      721 atctccatgc cgcgaataat cggcccagga tgcatgatca gcgccttcgg tgcctgcgca
      781 gcgatgcgat ccccgttggc ctgataacgg atgcggtaat catccaaatc gatcgaaagg
      841 cctgcgagcc gttccgcctg aatgcgcagc atcatcacga tcgtcgacgc gcgcagcgcg
      901 gcatcgaagt cgcgctcgat catcagtccc ggcctcatgc aagcagcagt ttccggcagc
      961 agagccggcg gtccgcacaa aatgaccttc gcccccagcc ggggaagcag cagcgcgttc
     1021 gaccgtgcca cgcgcgagtg gaggatgtcc ccgacaatgc tcacggtgac gccgcgcagc
     1081 accttctcgc tgatcctgcg cgccgggatc cccaggaact ggagcatcgt ccgcaaatcc
     1141 agcagcgcct gcgacggatg ctcggccatg ccgtcccccg cattgagcac cggcaaacct
     1201 gtggcgcgag cgagcaaata cggtcctccc gagtaagggc tgcgcaggat gatgcactcc
     1261 gcacccagcg ccttcagcgt gatccctgtg tctttcagcg attcgccctt ttcgatactg
     1321 gaggagaggg aactgaccag agttgtgtcg gccccgagcg ctttcgcggc aagctcaaaa
     1381 gacgttcgcg tccgcgtgct cgactcgtag aagagcagag cgacacggcg tttcgcgagg
     1441 cgctcggcgc ggcggaaggg attttccttt tcgagggcgt cggctgtctc ctggattcgc
     1501 cggacgtcag ccaccgcaaa atctgcggct gaaatggccg aaccaggagg aatgggttgc
     1561 tggaaaaata acggggccga gggtttttgc ggtggtttgc ggattgcggc cctggtcagc
     1621 gccattctcc ttcagctttc tgcgaatcca cgctagacac gttcgacaag cagcacctgc
     1681 tcgtcgttgt cgatctcttt cagcttcact tcgatgatct cgcgctgcga ggtcggaacc
     1741 gatcgcccta caaatgtcgc ctcaatgggc agctcgcgat gcccgcggtc gatcaggacc
     1801 agcagctgta cgcggcgcgg gcgtccatga tcgaacagag cgtccagcgc agcgcgaatc
     1861 gtccggcccg tgtaaagcac atcgtcaatc aggatcacat cgcggccgtt cacgtcgaag
     1921 ccgagcgagc cgggcacgac caccggccgc acgtcgcgcg tggagaggtc gtcgcgatag
     1981 aagctgatgt ccagcacccc ggtgtcgacc tgatgttttt cgatgccgcc gatgagggtg
     2041 gcaaggcgct gggcgatcgg caccccgcgg cgtttgatcc cgaccagccc gatcgtctcc
     2101 gcgccgttat tcttctcgat aatctgatgg gcgagacgga ccagcgtgcg ctcgatctcc
     2161 gaggccgaca tgatgcggcc cttctcgcgc aattttggcg tagcggtagc ctgcagctct
     2221 gacataggcg taaaggcgat catacggtgc cgtttgcgac gccgcaagac gaagagtgac
     2281 gcattaaggc gcagttttcc tcagaatgga acggtccgcg tttcgcggac ttacatcgtg
     2341 cgctgcgtcc gtggtcgtac ggaaagaatc cggccggcaa tggcgccgcc cgtagccgcg
     2401 aacaggatca tgccggccga aaccattgac gcagtcagca gctggatcgc tgcgtggcca
     2461 tcggccgaga gccagaagtt cgcccagttg tggaccagct ggggcgcctg ctgcatcgcc
     2521 tggctgttgc tcttcagagc ctgatcggcc agttgctgtg cgacgctcgc gaattgaccg
     2581 tcgatctcag cgccgtgatg cagaaaatag cgttcgacca ggaggcgaag cccatccgcg
     2641 agcgtcgcaa tcgaggccgc ggcagccccc agaatggcgc ccacgcgcca gccgaggctc
     2701 ccgtccgtga gagccgcggt tcgcctgcga tacaacgcga ttgcgccgaa gccgcccgcg
     2761 ataacaaaaa ccgagctcat gcctgcgaag gcggagagca gaccgacggg cacagcaaga
     2821 agaagagagt atccgatcgc cacccgccac tccacccgcg acggatccgt gcccaggcgc
     2881 tgcgcaggct gctgcggtcc ggtgggctcc gacgtatcga ccaccagttg gggagcgccg
     2941 cagtgggggc aaaagctttc gccctcatgc agggtgtttc cgcagcgatg gcacaacaac
     3001 tccatacttc gaccctatcg cacgcggaca gggagcgcaa tgcacgattc agtgaagcgg
     3061 aaaaagagct gccggccagg gcgtaccctc agtcgctggt cttcgacgcg tgctcgtcga
     3121 gcccgctggg caactgcaat tcaaccagag cgaaccgggt gcccggtcct ccatttttaa
     3181 tggcaaagat gtgctgatga tgtttggagc cggcgcgcag tgaaaactcg tcgttgttgt
     3241 cgaaaccgtt gccctgaacc ttgaccttcg aatcttcgct gcagctcagg ccctccgacg
     3301 tgatggtgtg cgcgccgacc gggtggtttc cctggcattc gagcacgtcg ccaaaccggc
     3361 ccatcgcatt cttgtagaag gccaccactt tgtcgcgatc gtcactgctc tcatagctga
     3421 cgacgcggac ccgaagctgg aactgcccga acccaaagtg cacatccgcc gacttgtcgc
     3481 cctcgtgatc gggcgagacc tgcgctccgg gataagccgg caggccaaga tcgagagcgg
     3541 tggtctgatc gctgcggacg tgaaggccgc ccagtggcgt gtcgatcctg acgtttttct
     3601 tctcgccgtt cttccccttg tccaccgaga cgtggcagcc gacggcggcc agcagcgcgc
     3661 caaggatgaa tgcttccggt aacaggcgca cgcgcaaacg cataggcaac tcctcaggat
     3721 ccctttgaaa aacgatacgc ctggcagaga ggtttggttc ccgctgtttg gcggatgaaa
     3781 gtgctccgca acaatgaatt gggtcctccc aagccatctc ccgcgccgcc tgatggcatc
     3841 atccttaggg tgagctggtg cggtacgcat gccgttacca ggctccctgc ggaggtttcg
     3901 cgagccaacg cagcatgcat cacggcaggg gcgcaggttt tcgcgatttc ctctgcttgc
     3961 tcccgtcgca ggagaatcgc gccagacggg ggaccctgcc gcatttttcg ccgcttaccg
     4021 tcgagttccc cctgacaacc ccgcctgctc aggcggcatt tttcctctgc tacgcatcgc
     4081 agcagcgtgc gaggaatcat ggacagaacg agaacgattt acagggcggc cggcacgggg
     4141 ctggtggcgt tggtgacgct cgccgcagga tgcaacaaca agaacaagcc gacacccgaa
     4201 aactttatga agacgatgaa cgcctatttc caggaccgca acgactgcct gttcccacag
     4261 ggacacacct ggccttatga agtagccccc agccccacag cgaaggacga caagaagcag
     4321 atggacgccc tcaccgatgc cggcttgctc acccggctcg aagatcgatc gatgaacgtg
     4381 tcccgttact cactcaatgg gctggccgga aagcggtttg ccccgcgctt ctgctacggg
     4441 caccgagaag tgacgtcgat tgtgagcaac acgccgccgg gaccgaagaa cggctttacc
     4501 gaaacggacg tgacgtatga gtaccagatg cgcgatgtgc cggtgtgggc gaactcggac
     4561 gcggtgaagg ccgcctttcc ccaactgggg aagtcgctgg gcaatacacc aaccgaccag
     4621 atgaccctgg cgaccgtcgg agccggttgg caggtgccgc ggtagaagag agttctaatc
     4681 agggtccgat tttcgaatca gcataccgac tcccatcagc gcaacgccgt agcacatccc
     4741 gattcctaac aactgcacga catccacggt gcggtacgac ttaaatcggt ctttcccgct
     4801 cagattcatc aatccgagcg agccgaagat aacaaccagc accaccgttg agataagggg
     4861 actctttcgt ttcgccatag ttcctctctt gctgaacgac aggcattgtg cgccgatttt
     4921 gtggaatcgt cgatcccgga attattttgg ggagttggaa ggattcaatg gtcgggacgg
     4981 gcagatttga actgccgacc cctcgcaccc caagcgagtg ctctaccagg ctgagccacg
     5041 tcccgacaac aacaagggtc gaattcagtt taccatccgc atccccccaa agtccgtgcc
     5101 accgcgcgac tcagcacacc caatgaaaac gcccgctccg tgctataaac ccgcatgccg
     5161 cgcttgcgga cttctctgct tgctgttgcc tttcccctcg ccctgatcgt tagttcgact
     5221 agatccagcc ccgcggcggc gcagaccgcc gactcgaagc cccccgcctc gatcgcggac
     5281 aaaaccaaat tcatggtgca catgcccgga ctcctcccac tggattggga cgcgaaggcc
     5341 ggcaagctct acctggaagt gccgctcgac ggcaacgccg gccacacccg ctcccaggac
     5401 atgatctgga ctaactccac gccgtggagc atgggcagca gccaactggg cggctatggc
     5461 atggaccgcg gacagatggc gccgggcgtg atcgtccgct tcgagcgaac cgggcccaaa
     5521 ctcctcctgg tgcagtcgaa cctcgacttc cgcagctcgt ccaccgacgc ggcggaacgg
     5581 gcggccgttg aggaatcgtt ccccgtctca gtcgtcgccg gcttcaccgt cgacgcggag
     5641 aattccgacg gcaccgtcct ggtcgatgcc acgcagttct tcctgaccga tgtgcatcac
     5701 gtcgccgaag ggctcctgca gggtaagcag ggaaccttcc acgtcgatcc ggcgcgatcg
     5761 accttcgtgc tgcaacacac gaaggcgttc ccgaaaaaca cagtggtgga agccgagctg
     5821 acgttcattc cgcgccccga ggatctccag cctgtcggaa aaattctgcg cgaagtctcg
     5881 cccgacgcgc gcgccgtgac cattcgcgaa cgccaaaatt ttgttgagct gccgccgccc
     5941 ggctacacgc cgcgccgatt ctctccgcgc gcgggctact tccccatgag ctatcgcgac
     6001 tacgatgcgc cgctcagcgc gccgctcacc gagcagttca tcctgcgcca ccgcctcatc
     6061 aaaaaggatc cgaactgcac acacgcctgc gaggcggttg cgcccattca gtattacgtc
     6121 gatcgcggcg cgcccgagcc gttgcgcagc gcgctggtcg aaggcgcgcg ctggtgggat
     6181 caggcctatc aggccgccgg ctgggcgccc ggtacgttca aagtggatgt gctgccgcag
     6241 gacgccgacc ccatggacgt ccgctacaac atcatccagt gggttcatcg ttacacgcgc
     6301 ggctggagct atggagacgc gatggccgat ccgcgcacgg gcgagatcat caagggcaac
     6361 gtgaccctcg gcagtcttcg cgcccggcag gattatctga ttgccgaagc gctgctggct
     6421 ccatacgaaa agggcaagcc gccggcggat gaagcgcacg accctgcgct gcagatggcc
     6481 ttgcagagaa tccggcagtt gggcgcgcat gagaccggac acacgctcgg cctcgcgcat
     6541 aactttgccg cgagcgcctt tccgcactcg ccggaccaga ccatgtccgt gatggattat
     6601 ccgcatccgt gggtcacgct gggtccgaac ggggtgcccg acctgactca tgcctatggc
     6661 gtcggcattg gaacctggga caaagtcgca atcgattacg ggtaccgcga gttcgatcgc
     6721 gacggcaagc cggtcgagga cgccgcggcg ctcgaca
//
LOCUS       DASZYN010000052         1578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_709264, whole genome shotgun sequence.
ACCESSION   DASZYN010000052 DASZYN010000000
VERSION     DASZYN010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709264"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1319)
                     /locus_tag="VHE33_01475"
     CDS             complement(join(175..1052,1052..1319))
                     /locus_tag="VHE33_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611217.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HVT96144.1"
                     /translation="MAGRKRHSAEDIVRKLRRADELAAEGKTGEEIAAELEVSAATLY
                     NWRRAYGGMDPDAAKELKELREQNARLKRLLAEAELVKDALREVAKGKILSPAAKRRA
                     VDMLKDTMSMSERLACKAVGLSRSTHRRLPQAQTPADPDADVRAWLRSYATKHPCHGF
                     RRAWAALRHDEGRQINVKKVHRLWCQEGLQRRVHSPRKRAGTSSVPEVVADAPKVVWA
                     LDFQFDSTTDGKAIKIASMVDEHTRESLLHLVERSITGERLVVELEKVFTAAGGPPKV
                     LRMDNGPELVSQALQRFCENKVGMSYIPPGTPWNNGYIESFNNRLRKECLNRNHWKTL
                     FEARVVIGDFKDEHNHRHRHSALGYRTPAEYAARCSHTHYPVACEIN"
BASE COUNT          258 a          504 c          477 g          339 t
ORIGIN      
        1 ggtcagcgtc actatccggg tgtgtactgg tcggctacgc agcgtgatca tgtgatctac
       61 gagtcgcgac ttgagttagc gaacctgctt ttggcggact ttgatcctgc ggtactggcg
      121 agtccccgat actgagtcca cccggttcta gagccaggtt gttgttgttc ggattcagtt
      181 gatctcgcag gccacggggt agtgggtgtg gctgcatcgg gcagcgtact cggccggtgt
      241 tcggtagccc agggccgaat gccggtgtcg gtggttgtgc tcatccttga agtcgccgat
      301 caccacccgg gcctcgaaca aggtcttcca gtggttgcgg ttgaggcact ccttcctgag
      361 tctgttgttg aatgattcga tgtagccgtt gttccatgga gtaccgggcg ggatgtagga
      421 catgccgacc ttgttctcgc agaaccgttg cagggcttgg gaaaccaact caggaccatt
      481 gtccatcctc agcaccttcg ggggcccgcc ggctgcggtg aacaccttct cgagctcgac
      541 gacgagtcgc tcgccggtga tggagcgttc caccagatgc agcagcgatt cacgggtgtg
      601 ttcgtcgacc atcgacgcga tcttgatcgc cttgccgtca gtggtggaat caaactggaa
      661 gtccagcgcc cacaccacct tcggggcgtc ggcgaccact tctggcaccg aggacgtccc
      721 ggctcgttta cgcgggctgt ggacccgccg ctgcagcccc tcctggcacc acaaccggtg
      781 cactttcttc acgttgatct ggcggccctc atcgtggcgt aacgccgccc aggcacgccg
      841 gaacccatgg cacgggtgtt tggtggcgta gctgcgcaac caggcccgca catcggcgtc
      901 gggatcggcg ggcgtctggg cctggggaag ccgtcgatgc gtcgagcgag acagcccaac
      961 cgccttgcac gccaaccgtt ccgacatgct cattgtgtcc ttgagcatgt ctacggcgcg
     1021 gcgcttggcg gccgggctca gaattttccc ttggctacct cccgcagcgc gtccttgacc
     1081 agctcggcct cggccaacaa ccgcttcaag cgggcattct gctcacggag ctccttgagt
     1141 tccttggccg catcggggtc catcccgccg tatgcgcgac gccagttgta caacgtcgcc
     1201 gcagacacct ccaactcggc ggcgatctcc tcgcccgtct tgccttcggc cgccaactca
     1261 tcggcgcggc gcagcttgcg cacgatgtcc tccgcggaat gccgcttccg accagccatg
     1321 tgtttcatcg ttccttccag cccgacatcg ggccacagga ctctaaaact ggttgggctc
     1381 attcagcggg aacacgccac cctcagctgc gtgagaggtc acgttcgggc tatttccgta
     1441 cacctgcgga gcgcttagca ttttgtacag caacgcgagg gaggcgaagt gagcggaccc
     1501 gagaacgtcg actttttcac cgacaagtcg gtcgtcgatg acccctacgc ctactacgac
     1561 gcgattcgtc gttgcccg
//
LOCUS       DASZYN010000053         4993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_709396, whole genome shotgun sequence.
ACCESSION   DASZYN010000053 DASZYN010000000
VERSION     DASZYN010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709396"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VHE33_01480"
     CDS             complement(<1..281)
                     /locus_tag="VHE33_01480"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HVT96145.1"
                     /translation="MFCFAVAAVAQDSTPASAPAQTPPPAAAAAPVWSVGGLDFSGYL
                     EGYYSYNANRPSNAANGQTNTLYNFNDKTDQFNLSGADLAINHSPDPIGV"
     gene            725..1873
                     /locus_tag="VHE33_01485"
     CDS             725..1873
                     /locus_tag="VHE33_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT96146.1"
                     /translation="MPDPHLPNARPLPDRPPMTLEEAGRIAAFVQAAARLDLHTKPGP
                     QLASLIRSIFDAEAVAIFDADLNETYKAGEWFADPEQSVRNICVFETVRDDPETGLIR
                     RVLRVREMPIGALQLRGELNASTSSTIATLIAITFDRYHSLANEMRMESAKKTEQLRT
                     TVLDSLAHAYKTPLTAIRAASSGLAEMGNLSPAQSELVSLIDEQTELLNQLTSRLLRT
                     ARLDGQALKVERVAIGPVIEDVVAAAREQLRGVVVNVSVVREDLSILCDRSLLQAMLT
                     QFVDNAAKYADVGTTVTITATEQSSAVVLSVHNRGPAIPGADHERVFDRYFRSAGAAN
                     HASGTGIGLSVAKQAALAHGGDVWVTSDTARGTTFFASFPATPQGVQA"
     gene            1870..2628
                     /locus_tag="VHE33_01490"
     CDS             1870..2628
                     /locus_tag="VHE33_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT96147.1"
                     /translation="MNRETTIRILLVDDEPAIRRALRTPLHELGFQTEEASRGEDALH
                     LIRTQPFDVVLLDINMPGIGGIRTLERMRAFAPRLPILMLTVRDDEEDKVEALELGAD
                     DYITKPFSVREVIARVRSAVRRAQTPERPEDAPIRIGKIEVQPARRTVKKSGQAIRLT
                     PKEYDILYYLMAHAGRAVTYGKLLSSVWGAQYREEVDYLRTFVRQLRKKIEDDPSNPV
                     YLVTDACVGYRFNEEIPKLRRDEPHAGEAYLPVT"
     gene            complement(2625..3602)
                     /locus_tag="VHE33_01495"
     CDS             complement(2625..3602)
                     /locus_tag="VHE33_01495"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HVT96148.1"
                     /translation="MSASATLEPGQQLDHFRIDACIAESGMASIYRATDVRDGRLVAI
                     KLPHASAEADPTLAERFRREEEIGTTLDHPNVMRTFPAREASRPYLVMEWVPGRLLRR
                     ILADEGKLPQERAIRIVSGILEALAYIHQRGVVHRDLKPDNIMVDERDHVKLIDFGIA
                     SKQGARRITYTGFSPALGTPDYIAPEQVQGKRGDQRSDLYAAGVMLYEMLTGKVPFSG
                     PSPLAAMNERLLRPPVPPREVNPSISAQLQEVIYRALEREPRNRYANAEEFLLDLRHL
                     DRVGVSERDELTDWRRRGSGKGSRSLIWIVLILIPLLLFVAMLLLAHRF"
     gene            complement(3606..4871)
                     /locus_tag="VHE33_01500"
     CDS             complement(3606..4871)
                     /locus_tag="VHE33_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96149.1"
                     /translation="MNANSVACLMLIAVLPMAPAGLALVNCGLSRSRSAAQSLVGALC
                     LVAVAAVVYFAWGFSLEGFAGGHGYSLHLGGADWNWAGAAPLFLRGLDDTSAPAAVFQ
                     IFAVSFVVLIPWGSGADRWKLGPACLLAIVLAGLVYPLFAHWVWGGGWLAQLGGTFQL
                     GAGFADPGGAATLQGTGGVAALAVVWILGPRRGKFPRGGPPAVIPAHHIIYVLFGCLV
                     ALVGWLALNLLGAIFFTQSGGAALAMTEVNTLLCASGALLAALLVTRLRFGKPDASLC
                     ANAWIAGLAASSAVAALVPPAAAVLVGVVAGAVLPPAIEFLELRCGIDDPSGGIVVHG
                     VSAIWGLLAVGFLSRSASGGQMIAQLVGIATLLGLVLPVVYGCSWLLNRVLPFRTHPH
                     GERIGMDLHELGSGAYPEFVMHTDEFIPH"
BASE COUNT         1018 a         1732 c         1412 g          831 t
ORIGIN      
        1 actccgatcg gatcggggct gtggttaatg gccagatcgg cgccggacag gttgaactga
       61 tccgttttgt cgttgaagtt atacagcgtg ttggtctgcc cgttggctgc attgctgggc
      121 cgattggcgt tgtagctgta gtagccctcc aggtatccgc tgaaatcgag accgccgaca
      181 ctccagaccg gagcggcagc agcagccgga ggcggcgtct gggcaggggc ggatgcgggt
      241 gtgctgtctt gagcaaccgc ggcaacggca aagcagaaca tcgagcttgc cgcaatcttg
      301 aatagaccac tcggcttgag gcgtatcagg gttgttcctc ccgtctgggt ggtgtgaact
      361 ctcgccgcgt cggcacccct gcgttgcgcc aggactagcg tccggttcct tgcaaggtgg
      421 cctgcgcgac gggttccttt atgtactcaa acaacccatg aggaagtcat aaggaattca
      481 cacagagttc agagtgctgt gacggaacgc ccgcgctgcg acacacgacg atgtaaaaaa
      541 cttgaattct catccggcat tatgcaaatc tgattgcaac tctggtataa ggccggagcg
      601 tttccggccc caatgttgat gacgccgttc tcctgagcgc gtcgtctctg tcgattccga
      661 actctgcgca gggaaaaagc tcttgactga aaacagatcc aaggctccag gctctcctgg
      721 agagatgccc gacccgcatc tccccaacgc ccggcccctg cccgaccgtc cgccgatgac
      781 cctcgaggaa gccggccgta tcgccgcctt cgtccaggcc gctgcccgcc tcgacctgca
      841 tacgaaaccc ggcccgcagc tcgcctccct gatccgttcc atcttcgacg ccgaggccgt
      901 cgccatcttc gacgccgatc tcaacgaaac ctacaaggca ggcgaatggt tcgccgatcc
      961 cgagcagtcg gttcgtaata tctgcgtctt tgaaacggtt cgcgacgacc ccgagaccgg
     1021 actgatccgc cgtgtgctcc gtgtgcgcga gatgcccatc ggagccctgc agctccgtgg
     1081 tgaattgaac gcctcgacca gcagcaccat tgctaccctc attgccatca ccttcgaccg
     1141 ctaccactcc ctggccaacg aaatgcgcat ggagagcgcg aagaagaccg aacagctgcg
     1201 cacgaccgtt ctggacagcc tcgcccacgc gtacaaaacg ccgctgactg cgattcgagc
     1261 cgccagcagc ggcctggccg aaatgggcaa cctgagcccg gcgcagagcg aactggtttc
     1321 cctcatcgat gagcagaccg agctactcaa tcaactgacc agtcgcctgc tgagaacagc
     1381 ccggctggat ggacaggcgc tcaaagtcga gcgagtggcc attggccccg tgattgaaga
     1441 tgtcgtcgct gctgcgcgcg agcagctccg cggcgtcgtt gtgaatgtat cggtcgtacg
     1501 cgaagacctc agtatcctct gcgaccgcag cctgctgcag gcaatgctca cgcaattcgt
     1561 cgacaacgcg gcgaaatatg cggacgtggg cacaaccgtc accattaccg caaccgagca
     1621 gtcctcggcc gttgttcttt ccgtgcataa ccgcggtcct gccatcccgg gcgccgatca
     1681 cgaacgtgtc ttcgaccgct acttccgttc cgccggcgcg gcgaatcacg cttccggcac
     1741 aggaatcggt ctctccgtgg cgaaacaggc cgcgctggcc cacggtggcg atgtctgggt
     1801 caccagcgat acggctcgcg gcacaacctt cttcgcatct tttcccgcca ccccccaggg
     1861 agttcaggcg tgaatcgaga aacgaccatt cgcatcctgt tggtagacga tgagcccgcc
     1921 attcgccgcg ctctgcgcac cccgctgcac gagctcggct ttcaaaccga ggaggcttca
     1981 cgcggcgagg acgcgctcca cctcatccgg actcagccct tcgatgtggt cctgctcgat
     2041 atcaacatgc ccggcatcgg cgggattcga acgctggagc gaatgcgcgc gtttgcccct
     2101 cggctcccca tcctgatgct caccgttcgc gacgacgaag aagataaggt cgaagcgctc
     2161 gaactcggcg ccgacgacta catcacaaaa cctttcagcg tgcgcgaagt catcgcccgt
     2221 gtccgctccg ccgttcgccg cgcccagaca ccggagcgcc ccgaggatgc ccccatccgc
     2281 atcggcaaaa tcgaagtgca gcccgcgcgc cgaaccgtga aaaagtccgg ccaggccatc
     2341 cgcctcacgc ccaaggaata cgacatcctc tactatctga tggcccacgc cggccgtgcc
     2401 gtgacctacg gcaagctgct cagttccgtg tggggcgcgc agtatcgcga ggaagtcgac
     2461 tatctgcgca cctttgtgcg ccagttgcgc aagaagatcg aggacgatcc ttccaatccg
     2521 gtctacctgg tcaccgacgc ctgtgtgggc tatcgcttca acgaggagat tcccaagctc
     2581 cgccgcgatg agccgcacgc cggcgaagcc taccttcccg tcacttaaaa tcggtgcgcc
     2641 aggagcagca tcgctacgaa cagcagcagc gggatcagga tcagcacaat ccagatcagg
     2701 ctcctgctgc cttttccgga gccgcgcctt cgccagtccg tcagctcgtc gcgttctgac
     2761 acgccaaccc ggtccagatg gcgcaggtcc agaaggaatt cctccgcgtt cgcgtaacga
     2821 tttctcggct cccgctccag cgcccgatag ataacctcct gcagctgggc actgatgctc
     2881 ggattcacct ctctcggcgg caccggagga cgcagcagcc gctcgttcat cgccgccagc
     2941 ggagagggtc ccgagaacgg caccttgccg gtcagcattt cgtacagcat cacgcctgcc
     3001 gcgtacaggt ccgatcgctg atcgccgcgc ttgccctgca cctgctccgg ggcaatgtag
     3061 tccggcgtgc ccagcgccgg cgaaaagccg gtgtacgtaa ttcgccgcgc gccctgcttg
     3121 gaggcgatgc cgaagtcaat gagcttcacg tgatcccgct catccaccat gatgttgtcc
     3181 ggcttcaggt cgcggtgcac gacgccgcgc tgatgaatgt aggcgagggc ttccagaatt
     3241 cccgaaacga tccggatagc ccgctcctgc ggcagcttcc cctcatcggc aagaatccgg
     3301 cgcagcagcc gtcccggcac ccactccatc accagatacg gccgcgaggc ttcgcgagcc
     3361 ggaaacgtgc gcatcacgtt gggatggtcc agcgtcgtgc caatctcctc ttcgcgccgg
     3421 aatcgctccg ccagcgttgg gtcggcctct gcactcgcgt gcggcagctt gatcgccaca
     3481 aggcggccat cgcgcacatc ggtggcgcga tagatcgacg ccattccgct ctcggcaatg
     3541 cacgcatcga tgcggaagtg atccagttgc tggccgggtt cgagggtcgc ggaagcgctc
     3601 atcggctagt gtggaatgaa ctcgtcggtg tgcatcacaa actcagggta cgcgcctgag
     3661 ccaagctcat gcaggtccat gccaatgcgc tcgccgtgcg gatgcgtgcg gaacggcaac
     3721 acccggttca gcaaccagct gcaaccgtag accaccggca gcaccagccc cagcagcgtc
     3781 gcgatgccca ccaactgagc gatcatctgc ccgcctgaag cacttctgct caaaaatccc
     3841 accgccagca atccccagat cgccgacact ccgtgcacaa cgatcccgcc ggagggatcg
     3901 tcgatcccgc aacgtagttc cagaaactcg atcgccggcg gcagaactgc ccccgccacc
     3961 acacccacca gcaccgcggc agccggtgga accagcgcag caacagcgct gctcgctgcc
     4021 aggcccgcga tccaggcatt cgcgcaaaga gaagcatccg gtttcccaaa acgcagccgc
     4081 gtcaccagca gagctgccag cagcgctccc gacgcacaca gcaaggtgtt gacttcagtc
     4141 atcgccaggg cggcgccacc gctctgggta aagaagatcg cacccagcag attcagtgcc
     4201 agccagccaa ccagcgccac caggcagcca aacagcacgt agatgatgtg gtgcgccgga
     4261 atcacagcag gcggcccgcc gcgcggaaac ttgccgcggc gcggtcccag aatccaaacc
     4321 accgccagtg ccgcgactcc tcctgtcccc tgcaaagttg ccgcgccgcc aggatcggcg
     4381 aaacccgcgc ccagttggaa cgttccgccc agttgggcga gccatccccc gccccatacc
     4441 cagtgcgcaa ataacggata aaccaatcca gccaggacga tcgccagcaa acaagcggga
     4501 cccagcttcc agcgatccgc tcccgatccc cagggaatca gtacgacaaa cgagacggcg
     4561 aaaatctgaa atacagccgc cggcgcagat gtgtcgtcaa gaccgcgcag gaacagcgga
     4621 gcggcccctg cccagttcca gtcggcccca cccagatgca aggagtagcc gtgcccgccg
     4681 gcgaagcctt cgagcgaaaa tccccacgcg aaatagacca ccgccgccac ggcgaccagg
     4741 cacagcgcgc ccactaaaga ctgcgccgca ctgcgcgagc ggctgagtcc gcagttcacc
     4801 agcgccagtc cagccggcgc catcggcagc acagcaatca gcatcaggca cgccacagag
     4861 ttcgcgttca tggccgcgct ccggctccgt caatgtgagc gcggcctgcc acgagcagac
     4921 ccaggcattc caccagcagg ccagcaacga taaaaccggc gcgcaaccca gcctgcggaa
     4981 gcaggcacaa tgc
//
LOCUS       DASZYN010000054         3012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_131223, whole genome shotgun sequence.
ACCESSION   DASZYN010000054 DASZYN010000000
VERSION     DASZYN010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131223"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..1748)
                     /locus_tag="VHE33_01505"
     CDS             complement(318..1748)
                     /locus_tag="VHE33_01505"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HVT96150.1"
                     /translation="MSTEYRNFLALSYEELEELNLKAKEQRRNRVDAGKIQEERLKFL
                     TDHKDIKAVTVLFSDLEGRLHMLDYDKKFLLKAYENLTFDGSSIRGFTAQKESDLRLG
                     IDWSAYYHAPADIFGAGKVLVFGDVIDKDGSPYGGDTRGLLKTFAGKQFEKNQYTLNA
                     ANEIEGFLFAGVDAERTFQTTGKFEYVNTGGYYHSLPGDPLRTFIDTVAEVQRAMGFE
                     NEKDHPEVAPSQFEINYGYGEVVAAADQIQLYKLICRQVATRLGMTASFLPKPVVGVN
                     GSGMHTNVSISKGGKNLFWDPKGQEKLSATGWEFVDRILTHGNDICLLLNSSVNAYRR
                     LDPHFEAPNQIKASAVDRGSMIRIPIGNERSMRVEVRSVAPDSNPYLVMLAVFQTGIE
                     GETSKAANLRQAERYLPDNIYTAIDDFRSASWTTKMLGEDVKSRYADLKQGSADRCAR
                     LLGTFVKPSEVQFHHEVYNQFLWNQF"
     gene            complement(1954..>3012)
                     /locus_tag="VHE33_01510"
     CDS             complement(1954..>3012)
                     /locus_tag="VHE33_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HVT96151.1"
                     /translation="PYCGSESAYFLAANRNKRSIAIDYDDGAGREVLERLLARADVFL
                     VNQPRLESLRRRSLDPESLLGRFPRLVVCSITGYGLSGPNAGQAGYDIIAQAEAGIMS
                     FTGSEGDPPIRYPVALADLICGVYSTTGILAALLARHRSGRGQFLDMALVDSQLTLLA
                     NIGSNYLNAGLLPEKWGNAHPSIVPYQLFEGRDGRYFVLGVATDVLWRRFVALVGIED
                     TIGAEARFATNPLRIQNREALLPLLQRVLLQRDASEWVTDFKKAAIPAALIQQVDEAL
                     GSEQAVARGLIVELEHPLLEHVRSIANPMRMSATPPTYRLPPPLLGEHTAAILSELGL
                     AVPEKAEEPIRHNPSSPS"
BASE COUNT          582 a         1039 c          800 g          591 t
ORIGIN      
        1 tccgtcaatg tgagcgcggc ctgccacgag cagacccagg cattccacca gcaggccagc
       61 aacgataaaa ccggcgcgca acccagcctg cggaagcagg cacaatgcag ccaccgcgat
      121 gatccatccg gaaaccagca aacctaagcc tgcaaccttc atcgtcgttt ccccgttgaa
      181 tctgtcgcga tcatatgcgc cccccacaga ccggaaaaga gaagcgcccc ggttcatcac
      241 cgaggcgccc tgttttgtct tgcgccgcag gcgcgtccgc ctggacggcc agtcccgccc
      301 gcctgggcgg ccagtcccta gaactggttc cagaggaact ggttgtagac ctcgtggtgg
      361 aactgcacct ccgacggttt cacgaacgtg cccagcaggc gtgcgcagcg atccgccgat
      421 ccctgcttca gatccgcata gcggctcttc acgtcctcgc ccagcatctt cgtcgtccat
      481 gacgccgacc ggaaatcgtc aattgccgtg tagatgttgt ccggcagata ccgctccgcc
      541 tggcgcaggt tcgccgcctt cgatgtctcg ccctcgatcc cggtctggaa caccgcgagc
      601 atcaccagat acgggtttga gtcgggcgct accgaacgca cttccacgcg catcgatcgc
      661 tcgttgccga tcgggatgcg aatcatcgag ccgcgatcca ctgccgatgc cttgatctgg
      721 ttcggcgctt caaagtgcgg atccagccgc cgataagcat tcacactcga gttcagcagc
      781 aggcaaatat cgttgccgtg cgtcaggatg cgatccacaa attcccatcc cgtcgccgac
      841 agcttctcct gtcccttagg atcccagaac aggttcttcc cgcctttgct gatcgagacg
      901 ttcgtgtgca tgccgctgcc gttcacgccc accaccggct tgggcaggaa cgatgccgtc
      961 attcccagtc gtgtcgctac ctgccggcag atcagtttgt agagctgaat ctgatcggcc
     1021 gcggctacca cttcaccgta tccatagttg atctcgaact gcgacggcgc cacttcgggg
     1081 tgatccttct cattctcgaa acccatggcg cgctgcacct ccgccaccgt gtcgatgaac
     1141 gtgcgcagcg gatcgccggg cagcgaatga tagtacccgc ccgtattcac atactcgaac
     1201 ttgcccgtcg tctgaaacgt ccgctccgca tccacgcccg cgaacagaaa gccctcaatt
     1261 tcgttggccg cgttcagcgt gtattggttc ttctcgaact gcttccccgc aaacgtcttc
     1321 agcaatccgc gcgtgtcgcc gccgtagggc gagccgtcct tgtcgatgac gtcgccgaac
     1381 accagcacct tgccggcacc aaaaatatcc gccggcgcat ggtaatacgc gctccagtcg
     1441 attcccaggc gcaggtcgct ctccttctgt gccgtgaatc cgcggatcga cgagccgtcg
     1501 aaggtcaggt tctcatacgc cttcagcaga aatttcttgt cgtagtccag catgtgcagc
     1561 cggccttcca ggtccgaaaa cagcaccgtc accgccttga tgtccttgtg atcggtgagg
     1621 aatttcagcc gttcctcctg gatcttgccc gcatctacgc ggttccgccg ctgttccttg
     1681 gcctttaggt tcagctcttc cagctcctca tagctcagcg ccagaaagtt ccgatattca
     1741 gtcgacatgc gtctccttga atgcaataga ttggtcgtcg ccattcgcga aaaccctcac
     1801 accgtacccc gcagccgcac cggccaatcc ggaccggaaa gtgcctgctg caagagacct
     1861 tatgaggaac gtggcgtttc gaagatcagg gtatcgctca cgatataaag atttccccct
     1921 ataaaaattt gtgatcgggg agattcgcgg cggttatgag gggctgctgg ggttgtggcg
     1981 aatcggttct tctgccttct ccgggaccgc taatcccagc tcactcagaa tcgccgccgt
     2041 atgctctccc agcagcggcg gcggcaagcg atacgtcggc ggcgtagcgg acatccgcat
     2101 cgggttcgcg atgcttcgaa catgctccag cagtgggtgc tccagctcaa cgatcagacc
     2161 gcgagccacc gcctgctcgc tgcccagcgc ttcgtccacc tgctgaatca gcgcagccgg
     2221 gatggccgcc ttcttgaaat ccgtcaccca ctcgctcgcg tcccgctgga gcagcaccct
     2281 ttgcaacagc ggaagaagcg cctcgcggtt ctgaatgcgg agaggattcg tagcgaagcg
     2341 cgcctccgcc ccaatcgtgt cctcgatgcc caccagagcc acaaaccggc gccacaaaac
     2401 gtccgtcgct acccccagca cgaaatagcg cccatctcgg ccctcgaaca actgatacgg
     2461 cacgatgctg gggtgcgcgt tgccccactt ctccggcagc aggcccgcgt tcaggtaatt
     2521 gctgccgata ttcgccagca gcgtcagctg cgaatcgacc agcgccatgt cgaggaactg
     2581 ccctcgcccg ctccgatgac gtgccagcag cgccgccagg atcccggtcg tcgagtagac
     2641 gccgcagatt aaatccgcca gcgccaccgg gtaacgaatc ggcggatctc cctcgctccc
     2701 ggtgaagctc ataatgcccg cctctgcctg cgcgatgatg tcgtagcctg cctggcctgc
     2761 gttcggcccc gacaacccgt atcccgtgat cgaacacacc accagtcgcg gaaaccggcc
     2821 caagagcgat tccggatcga gcgaccgccg ccgcagcgat tccagtcgcg gctgattcac
     2881 aaggaacaca tccgccctcg ccagcagccg ctccagcacc tcgcggcccg caccatcgtc
     2941 gtaatcgatg gcaatcgacc gcttattgcg attggccgcc agaaaatacg cggattcgct
     3001 cccgcagtac gg
//
LOCUS       DASZYN010000055         3914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_964886, whole genome shotgun sequence.
ACCESSION   DASZYN010000055 DASZYN010000000
VERSION     DASZYN010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964886"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VHE33_01515"
     CDS             <1..692
                     /locus_tag="VHE33_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HVT96152.1"
                     /translation="APVTFDVVSVKPAQPDPATAFYWRSTGDGFSATTTVQNLIQNAW
                     DFVVEDQIVNLPGWAKSDKFEIAAKMDPEAYAAFRKLSDEEQDRRWNRMMQAILADRF
                     HMQSHESRVLPAYALVLAKGGPKLKPSSAQGGGWSTGRGRITGHRMRLSDLADLLSHA
                     LARIVVDQTSLKGNYDISLTWTPDDERGLPDSGPSLFTALQDQLGLRLQTTKAPVASL
                     IVDHIEKPSAN"
     gene            complement(799..>3914)
                     /locus_tag="VHE33_01520"
     CDS             complement(799..>3914)
                     /locus_tag="VHE33_01520"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVT96153.1"
                     /translation="AVMRAQVVGATLAGRVQDRMGASLPGASVTVRNDETGAQRALTS
                     DADGRFSAPSLPVGAYSVTVAHSGFATQEQHGIALVVGQTRDLTVTLDLGTIQQQVVV
                     STQGPVINTSTQETSGLVDERQVKQLPLNGRSFDGLLTLNPATVNYTAERSGSVGTSN
                     SSVGNMFGVSGRRPQDNLFLLNGVEYTGASLINVTPGGTSGQLLGVDAVREFNVVTDT
                     YGASYGKRDGAQVSIVTMSGTNAVHGAGFEFLRNSALDARNYFDRGTIPEFQRNQFGG
                     SLGGPLRRDKLFLFGNYEGFRQNWGLSAVTLVPDARARQGYLPDSSGAEQYVGVNAAV
                     APLLALWPAANGPELGRGIAEAFSHPEQHIWEDFGTSRFDANLTSRDLLFAVYTVDDS
                     AAKTPTQNPLSLIDESLREQVASVQEQHTFTARLLNTARFGYSRASYIFNGYSPVAVP
                     GWVAGKPVGAIVISGSTASNGASQVTLAGTNVGSNNVTARNLFTADDHIYYSHGNHQL
                     EAGGWVQRIQSNDLLAQYQNGQASFSTLQSFLQGTVATFTVVPAPTKLGWRSLEGAAF
                     VEDTWKVRPRFELRAGFRSESTDGWNEAQGRASNYAIVGGVLQTDPVVGNAALTKNRA
                     KFLLNPRVGFAWDVRGNGKTAVRGGFGIYHGLLDTLDYRLDQTAPFNTAESIKNIAVA
                     DLHITPGTPPPAGALVSPSNVQPDIATPAVLTWSLRVEQQVAPQTSLTAGYVGSHSYH
                     QILSEDMNEPVPVFLADGSAFYPAGSKNANPQLANTTSWVSQGVGLYNALQVDVRHDL
                     AHGFQVRGDYTWSKNLDDGSAWNTSVSGNTPAFVEFPLRPKLDWGPGATDVRHVVSIN
                     GSWELPFGHGRAWFANVRGPARVATEGWSVSSIVTLQSGFPFSPQLGYNPTGNGDTRN
                     PVRPNWNSAFHGRLYPGTASQFFNPNAFLPPATGTYGNVRRDSLVGPGVEEIDFSATK
                     NTALSERVHLQFRAEFFNLLNHTNLLTPNEVVYTSATSGISPTAGVVTATSTTSRQIQ
                     FGAKVVF"
BASE COUNT          728 a         1471 c          985 g          730 t
ORIGIN      
        1 ccgcccccgt cacttttgac gttgtctccg tgaagcctgc ccaaccggat cctgcaaccg
       61 ccttttactg gcgctcgacc ggcgatggat tctccgccac caccaccgtc cagaacctga
      121 ttcagaatgc ctgggacttc gttgtcgaag accagatcgt caatctcccc ggatgggcga
      181 agtccgacaa gttcgaaatc gccgccaaaa tggatccaga agcttacgct gcttttcgca
      241 agctgtccga cgaagagcag gaccgtcgct ggaaccgcat gatgcaggcc atcctcgcgg
      301 accgctttca tatgcagtcg cacgaatcgc gggtcttacc cgcttacgct ctcgtcctcg
      361 ccaaaggagg acccaaactc aaaccatcct cggcgcaggg cggcggctgg tccacggggc
      421 gcggccgcat caccggccac cgaatgcgtt tgtcggacct cgccgatctc ctttcccatg
      481 ctctcgcccg catcgtcgtc gaccagacca gtctcaaagg caattacgac atctccctca
      541 cctggactcc ggacgatgag cgcggtctcc ccgacagcgg tccctcgctg ttcaccgcgc
      601 tccaggacca actcggtctc cgcctccaaa ccacgaaagc tcccgtcgcc tctctcatcg
      661 tcgaccacat cgagaagccc tcggcgaatt gacgatgacg gccagacgcg ccgctggcag
      721 tttcttgcta accgtacttc gctaagcgtt ctttgctacc gctgtctgct atccgctaac
      781 cgctaaccgc tgcctccttc aaaacaccac cttcgccccg aactgaatct gccgcgacgt
      841 cgtcgacgtc gccgtcacca cccccgcggt cggcgaaatc cccgacgttg ccgacgtgta
      901 caccacttca ttcggagtca gcagattcgt gtggttcagc aaattgaaga actccgcgcg
      961 gaactgcaga tgcacccgtt cgctcagtgc tgtgttcttc gttgctgaaa aatctatctc
     1021 ctccacgcct ggaccaacga gcgaatcccg ccgcacattc ccatacgtgc ccgtcgccgg
     1081 cggcagaaac gcattcgggt tgaagaactg gctcgccgtc cccggataca agcggccgtg
     1141 aaacgcagaa ttccagttcg gccgcaccgg attccgcgtg tccccattgc cggtcgggtt
     1201 atatccaagc tgtggcgaaa acgggaaccc cgactgcagc gtcacaatcg agctcaccga
     1261 ccatccctcc gtcgcgaccc gcgccggccc ccgcacattc gcaaaccacg cacggccatg
     1321 cccgaacggc agctcccacg atccgttgat cgacacgacg tgccgcacat cggtcgcacc
     1381 cggtccccag tccagcttcg gccgcagcgg aaactccaca aacgccggcg tgttgccgct
     1441 cacgctcgtg ttccacgccg acccatcgtc caggttcttc gaccacgtgt agtcgccgcg
     1501 cacctggaac ccatgcgcca gatcgtgccg cacatccacc tgcaacgcgt tgtacaaccc
     1561 cacgccctgc gaaacccacg acgtcgtatt cgccagctgt ggattcgcat tcttcgatcc
     1621 cgccggatag aacgccgatc catccgcgag aaacaccggc accggctcgt tcatgtcctc
     1681 ggaaagaatc tgatggtagc tgtgcgagcc cacatagccc gccgtcagcg atgtctgcgg
     1741 cgccacctgt tgctccaccc gcaggctcca cgtcagcact gccggcgtcg cgatatccgg
     1801 ctgcacattg ctcggcgata ccagcgcgcc cgcaggcggc ggcgttcctg gcgtgatgtg
     1861 cagatcggcg accgcaatgt ttttgatcga ctccgccgtg ttgaagggcg ctgtttgatc
     1921 gagccgatag tccagcgtgt ccagcagtcc gtgataaata ccaaacccgc cgcgtaccgc
     1981 cgtcttcccg ttgccgcgca catcccacgc aaatcccacg cgcggattca gcaaaaactt
     2041 tgcccgattc ttcgtgagag ccgcattgcc gaccaccgga tccgtctgca gcactccacc
     2101 cacaatcgcg taattcgaag cacgcccctg ggcctcattc catccatccg tcgattcgga
     2161 tcggaatccg gcgcgcagtt cgaaccgcgg ccgtaccttc cacgtgtcct cgacgaacgc
     2221 cgcgccctcc agcgacctcc accccagctt cgtcggcgcc ggcaccaccg tgaacgtcgc
     2281 taccgtcccc tgcaaaaacg actgcagcgt gctgaacgac gcctgcccgt tctgatactg
     2341 cgccagtaaa tcgttcgact ggatccgctg cacccacccg cccgcttcca gttgatgatt
     2401 cccgtgcgag taatagatgt gatcgtccgc cgtgaacaga ttgcgcgccg tcacgttgtt
     2461 gctgcccacg ttggtgcccg ccagcgttac ctgcgaagcc ccattggaag ccgtgctgcc
     2521 gctgatcaca atcgcgccca ccggcttgcc cgcaacccat cccggcacag caaccggcga
     2581 atagccgttg aagatgtagc tcgcccgcga gtagccaaag cgtgccgtgt tcagcagccg
     2641 agccgtgaac gtgtgctgct cctgcacgct cgccacctgc tcccgcaggc tctcatcgat
     2701 caggctcagc ggattctgcg tcggcgtctt cgccgcgctg tcgtccaccg tgtacacagc
     2761 aaagagcaga tcgcgcgagg tcagattcgc gtcgaaccgg ctcgtcccaa agtcctccca
     2821 aatatgctgc tccggatgac tgaacgcctc agcgatccct ctgcccagtt ccggcccgtt
     2881 cgctgccggc cacagcgcca gcagcggagc caccgctgca ttcacgccca cgtactgctc
     2941 cgcgcccgaa ctatccggca aatacccctg ccgcgcgcgc gcatcgggca ccagcgtcac
     3001 cgcgcttaag ccccagtttt gccgaaaccc ttcgtagttt ccaaacagga acagcttgtc
     3061 tcgccgcagc ggcccgccca gcgaacctcc gaactgattg cgctggaact ccggaatcgt
     3121 cccccgatcg aaataattgc gtgcgtcgag agcgctgttg cgcagaaact caaaccccgc
     3181 gccatgcacc gcattggtgc ccgacatcgt cacaatgctc acctgcgcgc cgtcgcgctt
     3241 gccataactc gcgccatagg tatccgtcac cacgttgaat tcccgcaccg cgtccacgcc
     3301 cagcaactgt ccgctcgttc cgcccggcgt tacgttgatc agcgatgccc ccgtgtactc
     3361 aacgccgttc agcaggaaca gattgtcctg cggacgccgc ccgctgaccc caaacatgtt
     3421 tcccaccgac gaattcgaag tccccacgct tcccgagcgc tccgccgtgt agttcactgt
     3481 cgccggattc agcgtcagca gcccatcgaa gctccgtccg ttcagcggca gctgcttcac
     3541 ctgccgctcg tccaccagcc ccgacgtctc ctgcgtcgac gtattgatca ccggtccctg
     3601 cgtgctcacc acgacctgct gttgtatcgt tcccagatcc agagtcaccg tcaggtcccg
     3661 cgtttgtccc accaccagcg caatcccatg ctgctcctgc gtcgcaaacc ctgagtgcgc
     3721 aactgtgacc gaatacgccc ccaccggcaa cgatggcgcg gagaaccgcc cgtccgcatc
     3781 gctcgtcagc gcccgctgcg ccccggtctc atcgttgcgt accgtcacgc tggccccagg
     3841 caaactcgcg cccattcggt cctggactcg tcccgcgagc gtcgctccca caacctgcgc
     3901 ccgcattacg gcca
//
LOCUS       DASZYN010000056         2166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1030521, whole genome shotgun sequence.
ACCESSION   DASZYN010000056 DASZYN010000000
VERSION     DASZYN010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030521"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VHE33_01525"
     CDS             complement(<1..822)
                     /locus_tag="VHE33_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96154.1"
                     /translation="MVFRSVLAACTLAAFSSIGSLAQSTTPSSQQTPATQTQPQQNSP
                     QPPAPTAPPKLELHDLPPEAHTMTPAEQDKARQQEAMNAAMRLAALQAHWGPQMTTPG
                     LSLAMTEVSRTKTADGTQIKYHLTGSGFSPTDKLTLVRWPLDAQAKVLMGGISFDAKG
                     VAVCSDSPGADSLAAAAANLSAAPAPNATAPAKTQSAPAPPPSCVTTMKPEEPVEIET
                     TAAPGEAIRVALVTADRKRAAETSAIPFPIVNEDKSCKLQVILSVKDGGLVLLEGT"
     gene            complement(981..1799)
                     /locus_tag="VHE33_01530"
     CDS             complement(981..1799)
                     /locus_tag="VHE33_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT96155.1"
                     /translation="MAPSIERKMCTAPDGVEIVYSAAGAGEPALVFIHGGLANRQFWD
                     GELRRFADGYRVLAPDLPGHGESGYKRAKWGLPQFGADIAALVEAEHLQQVILFGNSL
                     GGPVAVEAALLLGDKALGIVGVDTFHTLTHHMSEEEAQKRAEAFRDDYAGSLHHMVLQ
                     LFHADADPGLLRDAERRMAHTPPEAACAMFLSLAGYRADESARRLTCPLRAINGDLYP
                     TDIAGVRAVKPDFAAKILEHTGHYPMLERPAAFDRAVAAVVEELSHHGVIVSGG"
     gene            complement(1921..>2166)
                     /locus_tag="VHE33_01535"
     CDS             complement(1921..>2166)
                     /locus_tag="VHE33_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96156.1"
                     /translation="SLTLKQLTGPRCSATINPPGGAFPVSLGDIPTSGKATAQFSLTF
                     KGCGPLATFVITAPWTQATYHTGTLLGVAAPYFGFAF"
BASE COUNT          370 a          628 c          752 g          416 t
ORIGIN      
        1 tgtgccttcc agcagcacca gtccgccgtc tttgacgctg aggatcacct gcagtttgca
       61 gctcttgtcc tcattgacaa tcggaaaggg gatcgcgctg gtctcggcgg cgcgtttgcg
      121 atccgctgtc accagcgcca cacgaatcgc ttcacccggg gccgctgtgg tttcaatctc
      181 gaccggctct tccggcttca tcgtggtcac gcaggaaggc ggaggggcag gggctgactg
      241 cgtctttgcc ggagccgttg cgttgggagc cggggccgca ctcagattgg cagccgcggc
      301 cgccaaggag tccgcgcccg gtgaatcgct gcagaccgcc acaccttttg catcgaagct
      361 gatgccaccc atcagcacct tcgcctgtgc gtccagcggc cagcgcacca gggtcagctt
      421 gtccgtgggt gaaaagccgg aaccggtgag gtggtatttg atctgggttc cgtcggcggt
      481 cttggtgcgg ctcacctcgg tcatggccag agacaagcct ggcgtggtca tctgcggacc
      541 ccaatgcgcc tgcagggcgg cgagccgcat ggcggcgttc attgcttcct gttgccgcgc
      601 tttgtcctgt tcggcaggcg tcattgtgtg cgcctccggc ggcaggtcgt ggagttccag
      661 cttcggcggc gcagtgggcg ccggtggctg cggggagttt tgttgtggct gggtctgggt
      721 tgccggagtc tgctgcgaag agggcgtcgt ggactgcgcg agcgaaccaa tgctggaaaa
      781 cgctgcgagg gtgcacgcag ccagtacgga tcggaacacc atatccctga gaatagtgca
      841 aaccaggggg ctcgcgcgcc ctgatttacg tctgaacgcg ccggggcacg ttcacaaccg
      901 caggttgcgc ggcaggctgt cgcttctttc gtcgaaatgt tgcagcaggg atcgggtttt
      961 tgggatctcg gcctggacga ttaacctccc gaaacaatga cgccatgatg gctgagttcc
     1021 tccacgaccg cggccacggc acgatcgaag gcggcggggc gctccagcat gggatagtgc
     1081 ccggtgtgct cgaggatttt cgcggcgaaa tccggtttga cggcgcgcac accggcaatg
     1141 tcggttggat agagatcgcc gttgatggca cgcagagggc aggtgagccg gcgcgctgat
     1201 tcatcggcgc ggtagccagc cagggagagg aacatcgcgc aggccgcttc cggcggcgta
     1261 tgcgccatgc ggcgttcggc atcacggagc aggccaggat cggcatcggc gtgaaagagc
     1321 tggaggacca tgtggtggag gctgccggcg tagtcgtcac ggaaggcttc ggctcgcttc
     1381 tgggcctctt cttcgctcat gtggtgggtg agagtgtgaa aggtatccac gccaacgatg
     1441 cccagggctt tgtcgccgag cagcagagct gcctcgactg cgaccggacc accgagggag
     1501 ttcccgaaga ggatgacctg ctgcagatgt tcagcttcga ccaacgcggc gatgtcagcg
     1561 ccgaactggg ggagtcccca cttcgctcgc ttgtagcctg actcgccgtg gccgggcaga
     1621 tcgggggcca gcacgcggta tccgtcagca aagcgtcgca gttccccatc ccagaactga
     1681 cgattggcca gtccgccgtg gatgaacacc agcgccggct cgcctgcgcc ggcggccgag
     1741 tacacgatct cgactccgtc tggagcggta cacattttgc gttcgatcga tggggccatc
     1801 acacacctct ctcgatgagg acgatactcc gttcgtttag cagtagaagc gattttgaca
     1861 gggcgaaaag aaggacccgc ccgaaggcgg gtccaggttt tgagacgttg agcggagcct
     1921 ttagaaggcg aatccaaagt acggagccgc cacgcccagc agggttccgg tgtggtatgt
     1981 ggcctgcgtc cagggtgcgg tgatcacgaa ggtcgccagc ggtccgcagc ccttgaaggt
     2041 cagggagaac tgcgcggtcg ccttgccgct ggtggggatg tcgccaaggg aaaccgggaa
     2101 ggctccgccg ggcgggttga tggttgcaga gcagcgtgga cctgtgagct gcttcagggt
     2161 caggct
//
LOCUS       DASZYN010000057         4646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_964927, whole genome shotgun sequence.
ACCESSION   DASZYN010000057 DASZYN010000000
VERSION     DASZYN010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964927"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="VHE33_01540"
     CDS             <1..1250
                     /locus_tag="VHE33_01540"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVT96157.1"
                     /translation="VVSPESEGFSSERLENLHRLIQGEIDAKELAGAVTILARHGKVV
                     DYRTYGVKDVATGAAMTKDTIFRDYSMTKPVTGVAMMILYEQGKWLPWDPIAKYLPEF
                     ANLKVFDGFSPDGKMVLADPVHAPTMAELMSHSAGFSYGSGHGPVDALYGEVKPMQAT
                     NLQEFVERMAKLPLNYQPGKAWMYSASMDIEGAIVEKLSGQTLPEFMRTHIFEPLGMK
                     DAGFYVPAEKRARFATNYRADENEKLTVTQAGSPPNDYTAEPGMPSGGGGLVSTPEDY
                     YRFAQMLANGGELDGTCRPGERACAPARILSPQTVKLMTSNHLPAELLTGHWGIGQHI
                     MRPGFGYGFNCAVVFDPGAAGLPDGKGTFFWDGAAGTWFWVDPTNDVVFVGMIQRMGG
                     HDNHTLLYKSHAAVYSALVDPSK"
     gene            1607..2476
                     /locus_tag="VHE33_01545"
     CDS             1607..2476
                     /locus_tag="VHE33_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96158.1"
                     /translation="MTLCILGAAVVVSAPRCEAQEPPYFVTYSHAMEEPGNLEVAFKG
                     AQAAPRNANAFGSATVEFEYGAKAWWTTELYLDGQTTAHDSTICTGFRWENRFRPLLS
                     EHWINPVLYFEYENTSADRSFLEIVGNDSVDDLLLTNGQARPDVEREMEMKLILSSNA
                     KGWNFSENFITEKNLKISEGEPWEFGYAVAASRPLTLRAGARECAFCRENFAAGAEMY
                     GGLGTTDGFGLKSTAHYVAPTVEWNIPRGPTLKFSPNFGLNDNSMKVLWRVGVQYEFQ
                     QFFSRFHHARGVR"
     gene            2477..2854
                     /locus_tag="VHE33_01550"
     CDS             2477..2854
                     /locus_tag="VHE33_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVT96159.1"
                     /translation="MRRMIGLAVLVLGCAVLLAGAEVNQDWLKKVPAQERTRVNPYAG
                     QADAVAAGGRLFEDHCSRCHGADLEGANGKPSLKTAVVGNATDGELFWLLRNGDLRHG
                     MPSWSGLPEPQRWQIVAFLRSRK"
     gene            3012..>4646
                     /locus_tag="VHE33_01555"
     CDS             3012..>4646
                     /locus_tag="VHE33_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96160.1"
                     /translation="MRKAVKAVACAGMATVLTAGAAAVIAGCGSSTAPQFPAEDDVTP
                     AADSIGEALFLDTRFNEYFETHMTGVNDPLATGDPVVNQVQTSNGPMPGPFAGQSINC
                     RSCHFVTEFQDVKGAGNRTYADFTTRSPIPRPMNGFDHTQRNAMQMVGSLQPHTGPVF
                     LHFDGEFSGAVDLIESTITGRNFGWGPTQYKEALAHIARVIREDNGAGQLAADRLNGL
                     SYATIFLGTDSRIPRDLKLPASQRIDVTKASDQQILDEMALCISTYMKDLVFAQDEFG
                     RYIGSPYDVFLRVNHLPVQPKAGQSIADYNKELYGLVIALKNPVYVTEADGTFQYHDQ
                     KFQFGTLELAGLKVFLNQAPRATDGSQHAGNCAACHTAPDFTDFKFHNTGVAQDEYDS
                     VHGLGSFMALAIPGLAERNANYDLYLPITGNHPNATERFRREADAADPEYADLGVWNI
                     YLNPDKPNPQADLKSVLCAAGADCSVDQGLGRTIAAFKTPTLRDLEDSAPYFHNGSRL
                     KLDDVVNFYINSSGLARAGQLRNAPPEFAAMSISGDD"
BASE COUNT          932 a         1091 c         1738 g          885 t
ORIGIN      
        1 cggtggtgag tccggagagc gaggggtttt cgtcggagcg gctggaaaac ctgcatcggc
       61 tgatccaggg ggagatcgac gcgaaggaac tggccggggc ggtgacgatt ctggcgcggc
      121 atgggaaggt cgtcgattac cggacctatg gcgtgaagga cgtggcgacg ggcgcagcga
      181 tgacgaagga tacgatcttt cgcgactatt cgatgacgaa gccggtgacg ggcgtagcga
      241 tgatgatcct gtacgagcag gggaagtggc tgccatggga tccgattgcg aagtatctgc
      301 cggagtttgc gaatctgaag gtgtttgacg ggttcagtcc ggacgggaag atggtgctgg
      361 cggatccggt gcatgcgccg acgatggcgg agctaatgtc gcactcggcg ggcttctcgt
      421 atgggagcgg gcatgggccg gtggacgcgc tgtatgggga agtgaagccg atgcaggcga
      481 cgaacctgca ggagttcgtg gagcggatgg cgaagctgcc gttgaattat cagccgggca
      541 aggcgtggat gtactcggcg tcgatggata tcgagggagc gatcgtcgag aagctgagcg
      601 ggcagacgct gccggagttc atgcggacgc acatcttcga gccgctgggg atgaaggacg
      661 cggggttcta tgtgccggcg gagaagcggg cgcggtttgc gacgaattat cgggcggatg
      721 agaacgagaa gctgacggtg acccaggcgg ggagtccgcc gaatgactat acggctgagc
      781 cggggatgcc gtcgggaggc ggagggctgg tgtcgacgcc ggaggactac taccggtttg
      841 cgcagatgct ggcgaatggg ggcgagctgg atgggacttg ccgtccaggc gaacgcgctt
      901 gcgcgccggc gcggatcttg tcaccccaga cggtgaagct gatgacatcg aatcatctgc
      961 cggcggagtt gctgacgggg cactggggga tcgggcagca catcatgcgg ccggggtttg
     1021 gatatgggtt caactgcgcg gtcgtgttcg atccgggggc ggcggggctg ccggatggca
     1081 aggggacgtt cttctgggat ggcgcggcgg ggacgtggtt ctgggtggat ccgacgaacg
     1141 atgtggtgtt cgtggggatg atccagcgga tgggcggcca cgataatcac accttgctgt
     1201 acaagagcca tgcggcggtg tattcagcgc tggtcgatcc gagcaaatag ctgtgaaacg
     1261 gtcagctaaa gtccggtcag ctggtgaagc ggccagctaa gatccggtcg gtgtacagcc
     1321 gtcagccgag aggctggaat cctttgcggg ccagctgatg tgtcgcctcg cgacaaaaat
     1381 ggtacaaaac gccgcttgac atataggact gaagttgtcg tatattgatt cttcggaaga
     1441 tgacgtttcc ttcatcttgc gttgggcccc tgaaaacagg attgttatga gggtgtgatg
     1501 gagatcacat gctaaggcac tcctgcgcct ttaaatagga ccatttgagt cctatatcgg
     1561 agagaacttg atgcgattgg ccagtgaacg atgggttcgt gcagggatga cgctttgcat
     1621 tttgggggcg gcagtcgtgg tttctgcgcc tcgttgcgag gcacaggagc cgccgtactt
     1681 tgtgacgtac tcgcatgcga tggaggagcc gggaaatctg gaggtggcgt tcaagggagc
     1741 gcaggcggct ccgcgaaatg caaatgcgtt cggcagcgcg acggtggagt ttgagtacgg
     1801 cgcgaaggcg tggtggacga ccgagctcta tctggacggg caaacgacgg cgcacgacag
     1861 cacgatctgc acaggctttc gttgggagaa ccggtttcgc ccgttgctgt cggagcactg
     1921 gatcaatccg gtgctgtatt tcgaatacga aaacacgagc gcggatcgga gcttcctgga
     1981 gattgtcggc aacgacagcg tggacgacct gttgctaacg aacgggcagg cgcggccgga
     2041 tgtggaacgc gagatggaga tgaagctgat cctgtcgagc aacgcgaagg ggtggaattt
     2101 ttcggagaac ttcatcaccg aaaaaaacct gaagatcagc gagggtgagc cctgggagtt
     2161 cgggtatgcg gtggcggcga gccgtccgct gaccctgcgc gcgggcgcgc gggaatgcgc
     2221 gttctgccgg gagaattttg cggcaggcgc ggagatgtac ggcgggctgg gcacgacgga
     2281 tggtttcggg ctgaagtcga cggcgcatta tgtggcgcca acggtggagt ggaacattcc
     2341 gcgcgggccg acgctcaagt tttcgccgaa tttcgggctg aacgacaaca gcatgaaggt
     2401 gctgtggagg gtgggcgtgc agtacgagtt tcagcagttc ttcagccggt tccatcatgc
     2461 gagaggagtg cgatagatgc gcaggatgat tgggctggcg gtgttggttt tgggttgcgc
     2521 ggtgctgctc gccggcgcgg aggtgaatca ggactggctg aagaaggtgc cggcacagga
     2581 gcggacgcgg gtgaatcctt acgcaggaca ggcggatgcc gtcgcggcag gagggcggct
     2641 ttttgaggac cattgctcgc ggtgtcacgg cgccgatctc gagggcgcga atggcaagcc
     2701 gagcctgaag acggccgtgg tgggcaatgc gacggatggc gagttgttct ggctgctgcg
     2761 caacggcgat ttgcggcatg ggatgccgtc gtggagcgga ctgccggaac cgcagcgatg
     2821 gcagattgta gcgtttctga gaagcaggaa gtaaagagca gcgaagaagc ggtcagcgag
     2881 gagccggtca gcggagaatc ggttagctca aaaccggtca gcgaaaagcc taatagacga
     2941 tggcaagcga aaactcaccc cagtgagtgt gggcgacgtg tacgcccgaa atctgaaagg
     3001 aaaatggcac gatgagaaaa gcggtgaagg cggtggcgtg tgctggaatg gcgacggtgc
     3061 tgacggcggg agccgcggcg gtcatcgctg gttgcgggtc gagcacggcg ccgcagttcc
     3121 cggcggagga cgatgtgacg ccggcggcgg attcgattgg cgaggcgttg ttcctggaca
     3181 cgcgcttcaa cgagtacttc gagacgcaca tgacgggggt gaacgatccg ctggcgacgg
     3241 gcgatccggt ggtgaaccag gtgcagacgt cgaatggacc gatgccggga ccgtttgcgg
     3301 gacagtcgat caactgccgg tcgtgccatt ttgtgacgga atttcaggac gtgaaaggcg
     3361 cgggaaatcg gacctatgcg gacttcacga cgcgaagccc gattccgcgg ccgatgaacg
     3421 gcttcgatca tacgcagcgc aacgcgatgc agatggtggg atcgctgcag ccgcacacgg
     3481 ggccggtgtt tctgcacttt gacggcgaat tctcgggggc ggtggatctg atcgagagca
     3541 cgatcacggg acggaacttt ggatgggggc cgacccagta caaggaggcg ctggcgcata
     3601 tcgcacgcgt gattcgcgaa gacaacgggg cggggcaact ggcggcggat cggctgaatg
     3661 ggctttcgta cgcaacgatt tttctgggga cggattcgcg gattccgcgc gatttgaagt
     3721 tgccggcgtc gcagcggatc gacgtgacga aggcgagcga tcagcagatt ctcgatgaga
     3781 tggcgttgtg catttcgacg tatatgaagg acctggtgtt tgcgcaggac gagttcgggc
     3841 ggtatatcgg gtctccgtat gacgtgtttt tgcgggtgaa tcatctgccg gtgcagccga
     3901 aggctgggca gagcatcgcc gactataaca aggagctgta cgggctggtg atcgcgctga
     3961 agaatcccgt ctacgtgacg gaggcggatg gaaccttcca gtatcacgat caaaagtttc
     4021 agtttggcac gctggagctg gcggggctga aggtttttct gaatcaggcg ccaagggcaa
     4081 cggatggttc gcagcatgcg ggcaactgtg cagcgtgcca cacagcgccg gactttacgg
     4141 atttcaaatt tcacaatacg ggcgtggcgc aggacgagta cgacagcgtg cacggcctgg
     4201 gctcgttcat ggcgcttgcg attccggggc tggcggagcg gaacgcgaac tacgatctgt
     4261 atttgccgat cacggggaat cacccgaacg cgacggagcg atttcggcgt gaggcggacg
     4321 cggcggatcc ggagtatgcg gacctggggg tttggaatat ttatctaaat ccggacaagc
     4381 cgaatccgca ggcggatctg aagtcggtgt tgtgtgctgc gggcgcggac tgttcggtgg
     4441 accagggact ggggcggacg attgcggcgt tcaagacgcc gactctgcgc gatctggagg
     4501 actcggcgcc gtactttcac aacgggagcc ggctgaagct ggacgatgtg gtgaatttct
     4561 acatcaacag ctcggggctg gcgcgggcgg gacaattgcg gaatgcgccg ccagagtttg
     4621 cggcgatgtc gatcagcggg gacgat
//
LOCUS       DASZYN010000058        16865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1094966, whole genome shotgun sequence.
ACCESSION   DASZYN010000058 DASZYN010000000
VERSION     DASZYN010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 16865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16865
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094966"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VHE33_01560"
     CDS             complement(<1..717)
                     /locus_tag="VHE33_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HVT96161.1"
                     /translation="MAAGVRLPIPEPLPVVVTPEKIPHSDSFFDRDLKTSSDRFAILL
                     FVAALIAGLVYAGSQIARDLSGVHLLSAWPYILLGLALFIALGFEFVNGFHDTANAVA
                     TVIYTNSLKPNVAVVWSGIWNLIGVLTSSGAVAFTILSLLPVELILQVGKGPGFAMVF
                     SLLIAAVVWNLSTWWFGLPASSSHTLIGSIIGVGIANQLMNSRTGTSGVDWEQAAAVF
                     KVLLISPVIGFGCAALFLLIL"
     gene            complement(998..1930)
                     /locus_tag="VHE33_01565"
     CDS             complement(998..1930)
                     /locus_tag="VHE33_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HVT96162.1"
                     /translation="METIPNENPETKSNGQSTPEKTSPAASPKPSTAAAAPVSQPSRS
                     KEANRDNEGSFPFSVIVGQVYEGPYDLLLDLVRKQNIDIYDIPMAKITAQFLAYVEHL
                     KASDVDTAGEFIYMSALLIHIKSKMLLPRDPLEAAEGGTEDPRLELVERLLEHERFRN
                     AAQMLQQKRQLEEATWTNPGMREFREVADADQDIAADTLDLVRVFREILERAKNRPVL
                     DVSEDAVTVRQMIDFVRRRLMMEDRPVGLSKLLSNTRSTGAVVAMFLALLELVRLQAI
                     LLRQDRGFSEIYIKKHEQFEQVINERLSARDDWS"
     gene            complement(2007..3065)
                     /gene="trpS"
                     /locus_tag="VHE33_01570"
     CDS             complement(2007..3065)
                     /gene="trpS"
                     /locus_tag="VHE33_01570"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HVT96163.1"
                     /translation="MSNLVSDRKRILSGMRPTGKLHLGNYMGALHNWVRLQDQYECYF
                     FIADVHALTTDYADPSQIAANTREVILDYLAAGLDPAKSVLFVQSQVKQHFELPLLLG
                     MITPLGWLERVPTYKEQQENLASKDLTTYGFLGYPVLMASDILVYQADSVPVGQDQVA
                     HVELTREVARRFNQFYKRADGTEILPEPQVLLTPSPKLPGTDGRKMSKSYGNTILLSD
                     PEPEIRAKIKPMVTDPARVRRKDPGNPDKCPVGDLHKIFSTQETIAKVYDGCTTAGIG
                     CIECKSWVADAIVSTLAPIQERRVHFEQNPTLVTDILADGARRATARAEQTMHEVRSA
                     MGLAGTPFDSSSQRQGPA"
     gene            3199..3885
                     /locus_tag="VHE33_01575"
     CDS             3199..3885
                     /locus_tag="VHE33_01575"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HVT96164.1"
                     /translation="MKTAISVSKEDYLKAILEAESEGQTVISATLAHWLAVSAPAVSM
                     ALRRLKRDGYVEVRADGVVRLTPKGHETAYRTALRHHLIERMLSEMFGMPWYEVHDEA
                     ERLEHAVSPAFEALLMNKLGEKGTCPHGNAVLPESDAARKRHGVRPLAEVAEGIDYTV
                     TSLYERDEKLLRFLQQAGIEPGGSVRVVRRNYDQTVGIETAAGQFTIGPAAAEKVRVR
                     RRGGAGRNGH"
     gene            4003..7011
                     /locus_tag="VHE33_01580"
     CDS             4003..7011
                     /locus_tag="VHE33_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HVT96165.1"
                     /translation="MALTALPARALDPHTALAQYGYQSWQTDTGLPQNTVHAIVQGRG
                     GFLWIATEGGLVRFDGVEFRTYTRANTRDLPSDLIDDLMEDGAGTLWIGTSGGLARMR
                     DGKIESFNSDKGIPATQVWRTFEDRSGRVWALTASGLFRIEGNHAQRVALDAALTENS
                     RMVEGTNGSLWLGTADGLIHGNNKGEFRSGPNQGQEVVALATDSAGRGWAGTRSGLEM
                     CTEGGDCHRPAIPRSAGAIHSIVRDRQDRMWVGTDGGLFAITNRIVGVEATGRVDYLY
                     VDRQGMVWAGTAHGLMRVDPEKPETAEWLKGGDVFLSAAEDREGDLWLGTESGGLAVV
                     RDRKFSTLTAEDGLTDEYVLALAQTPNGQVWAGTKGGGLNVFRDGKFHQVTTEQGLSS
                     NVVLAVASASRADLWVGTPDGLDLLHDGEMARVFTTADGLADDFVRSLFVDSHGALWI
                     GTRHGLSRYVNGQFTTWTTLDGLGSDLVGAMAESKDGVLWIGTLGGLSRFREGKFTNL
                     TVKDGLSSNVVTALHEDSDETLWIGTNDGGLNRWRNGKVVSLAAAGIPHRLTGILEDG
                     RGYLWISSNSGVYRASRDGLNRMADGGPAVDVMRFGTADGMGISECSSGGHPAALRLR
                     DGTLWFATLKGIARVDPVHMPMNRVAPEVTIEQVSVDDVQQKADAKISVSPGHSHYEF
                     DYAGLSFVAPQKVEYRYQLVGFDRDWVDAGTRRVAYYTNLPHGRYTFRVIARNNDGVW
                     SEAAATAGLTVEPHLYQTVWFRLLVLAAIAALGYAAWKRRLLHVEREFQAVLGERTRI
                     AREIHDTLAQGFVAVSVHLELVAQLMRGSVDAAREQLERAQALVRSSLEDARTSIWEL
                     RSQSGEREDLAARILKTAEEVTGRAHSHARVQMHVTGTNHPLDEDVERELMRIAREAV
                     TNAVRHGDPENIGVRLEFEAGMFGMEIRDDGRGFTGTPADGASGHFGLTGMRERAEAI
                     GANLTVESSPGRGTSVRVGLVLKGETEKQT"
     gene            7014..7640
                     /locus_tag="VHE33_01585"
     CDS             7014..7640
                     /locus_tag="VHE33_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT96166.1"
                     /translation="MADTINILLVDDHHIVRQGLAALLKTVPGFAVVAEASDGEQAVE
                     AFRKHKPDVTLMDLRLPKVNGVEAIGRIRQEFPAARIIVLTTFDGDEDIYRAMQAGAK
                     GYLLKGMDLNELTDAVKVVHAGKSRIPSRVAEKLAERMGGASLTARELEVLQLIVAGK
                     SNKEIGNALFISEATVKTHVNSLLSKMGVEDRTQAATTALQRGIVHLD"
     gene            7693..8232
                     /locus_tag="VHE33_01590"
     CDS             7693..8232
                     /locus_tag="VHE33_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96167.1"
                     /translation="MMTVHGGKWLTGAAIVVMLALCAFAQEKRGSEWRVATDAELRSV
                     IPARAPVVQERIETELRSASGITNGHGKYIAGVVLITAGYSAEGKYSHFFLTQVPVRV
                     GNDTRLAAGEYVIGYEHVDNGELLIHFYQAATGRPVGDVAATLLTGTTRVESFRIWPP
                     GERAAIQIGRFGFSYRIGS"
     gene            8238..9434
                     /locus_tag="VHE33_01595"
     CDS             8238..9434
                     /locus_tag="VHE33_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HVT96168.1"
                     /translation="MAETLSQKEQKVVEALGENWQAEMRGFHTYNTLAERDDDPVRKK
                     ALRHMAEQEAHHAAMWAKRIRELGAELPKYEGRETGDADTLVNRLGGPQMALRRLEIE
                     ESRDIARYGQQLKDLGDEPSVAILKQVIADEQEHYRELSELVRHRYPKTANTSEMARQ
                     ELDELLTRRNRSGRQTAGWIGDAIYGVNDGLGAIFGIVSGVSGATLGNSKWVLLSGLA
                     GMIASALSMGSGAYLAAKSEREIYEAELTREREAIEMNGPEARELLSLYYQVKGIPRD
                     DADHVVEHLAESPQQFTRALAAERLNMTEEGLSQPWVSAWSGALSTAVGAFIPIVPFF
                     FMSGINAVLVSAIVSLIAHFAVGAAKSLITVRSWWSSGLEMTLVGAIEGAATYVIGIG
                     LGHIGA"
     gene            9483..10334
                     /gene="purU"
                     /locus_tag="VHE33_01600"
     CDS             9483..10334
                     /gene="purU"
                     /locus_tag="VHE33_01600"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HVT96169.1"
                     /translation="MLLIDCPDRRGLVAGVSSLLYRHGANILHADQHRDEELGLFFMR
                     VEWALDGNPGPSSFDMAAFELEFGSMAEAFQMRWRLTTSDHRTRVALFCSHYLHCVAD
                     LLHRWKSGELACDFSAIVSNHREAEPLANFYGIPFMHVAVTAATREAGEQRQLALLAE
                     KKVELVVLARYMQILSASFVSQFPSAIINVHHSFLPAFTGARPYHAAHSRGVKLIGAT
                     SHYVTEALDEGPIIEQDVARISHRDQVEDLVARGRDLERIVLSRAVRWHLERRILRYG
                     NKTVVFD"
     gene            complement(10413..13778)
                     /locus_tag="VHE33_01605"
     CDS             complement(10413..13778)
                     /locus_tag="VHE33_01605"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HVT96170.1"
                     /translation="MLRHTSAVLFALLVLPFASHASSFPATPESARARDAAPLLLLHP
                     SLSKDQIAFVHADDIWIVQRAGGEATRLTSTASVVDGPFFSPDGQTLAWSARIRGDID
                     VYTMPATGGVPHRLTYHPGDDRVVGWSPDGKDILFLSGRAAWNDFAQLFRVPADGSAL
                     PVALPLPSVDDASLSPDAKQIAYTPFNQWQVAWKRYHGGQTEPIWIVDLASLNLTKIP
                     RDNSNDKNPLWVGDKVYFLSDRNGPVSLFSYDTQSKQVEQVIENKSFDLKSAGAGPGG
                     IVYEQFGSIHLYDPATKTQHPVEITLHGDLPALEPSLAVIDPHTILNSAISPTGQRAV
                     FEAHGDIFTVPAEKGDARNLTDTSNAAERDPAWSSDGKSIAYFSDASGEYQMFIRDQD
                     GIAKPKAIDLGPAPSYFYGPRWSPDSKHILYADKKLNLWYLDVESPHPVKVDTQDFEG
                     FGFAGNSVWSPDSKWIAYLKELHNHMHAVFLYSLDTLKSNQITDGMSDIGSLAFDRNG
                     KYIYFTASTNVGPSVAGFDLSSLDRAVTSNAYIVVLSKDLPSPLPPESDDEKAKEENK
                     PAADEKKPDEKAAGEKKEESGKEKKDEKKPPVVKVDFDGIDQRILALPIPARNYIDLQ
                     AGKEGVIYLAEGSSVANPSQQNGAPIHALWRYALDKRETTEVLSGISAYTISANGEKM
                     LYRKGDAWLIASAEEVKTSPGKTLNLGNLQAHVDPRAEYRQMYNETWRIERDFFYDPN
                     HHGLDIAKVEKRYRPYLDGIGSRDELNYLFEEMLGEITVGHMFIRGPRTPDHSPKTGL
                     LGADYSVDHDRYKFAHIYNGENWNPALHAPLTQPGVNVREGEYLLAVNGRDLHAADNI
                     YSFFEGTAGKQVVLRVGPNPDGAGARDVTVVAVDSEHGLRNLAFIESARRKVDEMTHH
                     QVAYVYMPNTGGGGFTNFNRYFFAQVDKKGVIIDERYNEGGSIADNVIDTLRRVPMSR
                     YETREGEDVTDPQGAIFGPKAMLINQSAGSGGDLLPWYFRKAGLGPLIGVRTWGGLVG
                     IGGYPVLLDGGTITAPRTAIYGLKGQFEVENHGIPPDIEVEYLPKEVAAGHDPQLERA
                     AQYIMEQLKEHPLPTYPKPAYPNYHEHDGLGVH"
     gene            complement(13879..14745)
                     /locus_tag="VHE33_01610"
     CDS             complement(13879..14745)
                     /locus_tag="VHE33_01610"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HVT96171.1"
                     /translation="MIERIAILGTGLIGSSVGLALRAHGFSGSIAGWDPNPGQVHVAL
                     RRQAITVVADDPLAAAAEASLILLGGPVFTILEWLDRLAPFLRDDQLVTDVGSVKGVL
                     CEHAREKYNGPGQPLFLPGHPMAGKEVSGAENADADLFEDAVWLFTPGSHPEGGAPVV
                     QDWRTWVTRFGARIIDLDPARHDVLCAWISHLPQFVSTALAALLEDEFAANADLLPIG
                     GRALREMTRLGASPFSMWRDIAHTNSDAIARSLDALEQRIAHIRENLKTPQLRDEFDR
                     ANRFRLRDPRNP"
     gene            complement(14795..15811)
                     /gene="aroF"
                     /locus_tag="VHE33_01615"
     CDS             complement(14795..15811)
                     /gene="aroF"
                     /locus_tag="VHE33_01615"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HVT96172.1"
                     /translation="MIVAMQERATEEEIQAVVERMMEIGFNVHRTTGTVQTILAGVGT
                     PGAFSHDEFEVLSGVAEVIRISSPYKLAGRSFRPEGTIVRFPNGVCVGGHEVVVMAGP
                     CSVESREQIFACAKLVKDAGAKFLRGGAFKPRSSPYSFHGLGLDALKLLREAGDAHGL
                     LVVSEVMEISQIELMMPYVDLFQVGARNMQNFNLLRELGDVRRPVLLKRGIAATIEEL
                     LLSAEYLLAGGNYDVILCERGIRTFETYTRNTMDISAIPVVKKLSHLPIVGDPSHGTG
                     RRDKVPAMARATVAAGGDGVLIEVHPNPDKALSDGAQSMFPDQFAKLMDSLRVIATAV
                     ERTI"
     gene            complement(15808..16131)
                     /locus_tag="VHE33_01620"
     CDS             complement(15808..16131)
                     /locus_tag="VHE33_01620"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HVT96173.1"
                     /translation="MDIAAWRRKIDELDRQIVLLISERAAAAQEIGRLKRTTDLPVYE
                     PNRERVIFENVRTHNPGPLPDIELVHIYERIVDVMRALQRNELASRPGIPASEAHSKQ
                     TGTDQ"
     gene            complement(16241..>16865)
                     /gene="trpA"
                     /locus_tag="VHE33_01625"
     CDS             complement(16241..>16865)
                     /gene="trpA"
                     /locus_tag="VHE33_01625"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HVT96174.1"
                     /translation="QRASERAVKNGTRLHDVLALAADLRLERPGAGLIIFTYYNPVLR
                     FGLENFCAAAEQAGIDGVLLIDMIVEEAGEYLQALHRHRLAPIFLVAPTSPDDRLEKI
                     AAVSQGFVYAVSRTGITGAQKSLASDAEHLVRRLRRFTRLPIAVGFGISTPEQFATVG
                     EFADAAAIGSALVATIEQAGPQQAANSIARFIQGLRGVAKEPAVPAR"
BASE COUNT         3181 a         4194 c         6304 g         3186 t
ORIGIN      
        1 gaggatgagc aggaaaaggg cagcgcagcc gaagccgatg acgggagaga tgaggagaac
       61 cttgaagacg gcagcggcct gttcccagtc cacgccgctg gtgccggtcc gggagttcat
      121 gagttggttc gcgatgccaa ccccgatgat cgagccgatc agtgtgtgag aactggaggc
      181 gggcaggccg aaccaccagg tgctgaggtt ccacacgact gcggcgatga gtagcgagaa
      241 gaccatggca aagccggggc ctttgccgac ctggagaatt agctcgacgg gcagcaggga
      301 gaggatcgtg aaggccaccg cccccgacga agtgagcact ccaatcaggt tccagatgcc
      361 agaccagacg acagcgacgt tgggcttgag cgagttggtg tagatgaccg tggctacggc
      421 attggccgtg tcatggaaac cgttgacgaa ttcaaatccc agggcaataa agagcgcgag
      481 gccgaggaga atatagggcc aggcggaaag gagatggact ccggacaggt cgcgggcgat
      541 ctgagagccg gcgtagacca gaccggcgat cagggccgct acaaaaagca ggatcgcgaa
      601 gcgatccgat gaggttttta ggtctcggtc aaaaaatgaa tcggaatgag gaattttctc
      661 cggggtgaca acaaccggaa gcggttccgg gatggggagc cttactcctg cagccataaa
      721 aatggtggtg cgcccttagt gtagagggcc ggagggaggt gaggccacgg taccaacgga
      781 gattaccaca ataacggacc gacacaactg gtcctgctcc ggaaaacaaa gggtttatgg
      841 gccatagggt atccggaatc aggagcccac cctcgttttg aagcagggcg tgaggtggac
      901 tctgggcgaa aaacgtcgcg gacctccagt caagggtccg ttttcgtcgg tgatcgggca
      961 aatccttaaa aaatcagccg atatgctcag gacgacgcta gctccagtcg tcacgagcgg
     1021 agaggcgctc attgatgact tgctcaaact gctcgtgttt cttgatgtag atctcgctga
     1081 agccgcgatc ctggcggagc aaaatcgctt gaaggcggac caactccagc aaagcgagga
     1141 acatagcgac gacggcgcca gttgagcgcg tgttagaaag cagcttggat aggccgaccg
     1201 gccggtcttc catcatcagg cgccgacgca cgaagtcgat catctggcgc accgtaacgg
     1261 catcttccga gacgtcgagg acaggtcggt tcttcgcgcg ctccagaatc tcgcggaaga
     1321 cacggaccag gtccagggtg tcagcggcga tgtcctgatc ggcgtcagcg acctcgcgga
     1381 attcgcgcat gccgggattg gtccaggtgg cctcttcgag ctgacgcttc tgctggagca
     1441 tttgcgcggc gttgcggaag cgctcgtgtt cgaggaggcg ctcgaccagt tcgagacggg
     1501 ggtcttccgt tccgccctcg gccgcttcga ggggatcgcg cggcagcagc atcttgctct
     1561 tgatgtggat cagcagggcc gacatgtaga tgaactcgcc ggccgtatcg acgtcggagg
     1621 ctttgaggtg ctcgacatag gcgaggaact gggcggtaat cttcgccatg ggaatgtcat
     1681 aaatatcgat gttttgcttg cggacgaggt cgaggagcag gtcgtagggg ccttcgtaga
     1741 cctgaccgac gatgacggag aaggggaagg aaccttcgtt gtcgcggttg gcctccttcg
     1801 aacgcgaggg ctgggaaaca ggtgcggctg cggccgtgga tggtttcggt gatgcggccg
     1861 gcgaggtttt ctccggggtg gactgtccgt tggatttcgt ttccgggttt tcgttgggga
     1921 tggtttccaa tgtttccctc aagggctgaa gcccgactcg atcatgcact gtacggcacg
     1981 gctgaggcca cgccctttca aaacaactaa gccggaccct gacgttggct ggacgaatcg
     2041 aacggagttc cagcgagacc catggcggag cggacctcgt gcatggtttg ctcggcacgg
     2101 gcggtggctc ggcgtgcgcc gtcagccagg atatcggtga ccagcgtagg attttgctcg
     2161 aagtgcacgc ggcgctcctg gatgggcgcg agagtggaga cgatggcgtc ggcgacccag
     2221 cttttacatt cgatgcagcc gataccggca gtcgtgcagc cgtcgtagac cttcgcgatg
     2281 gtttcctggg tggagaagat cttgtgcaga tcgccgacgg ggcacttgtc agggttgccg
     2341 ggatccttgc ggcggacgcg cgcggggtcg gtgaccatgg gcttgatctt cgcgcggatt
     2401 tccggctccg ggtcggagag caggatggtg ttgccgtagc tcttggacat cttgcgcccg
     2461 tcggtgccgg ggagtttggg ggatggggtg aggaggacct ggggttcggg gaggatctcg
     2521 gtgccgtcgg cgcgcttgta gaactggttg aagcggcggg cgacttcgcg ggtgagctcg
     2581 acgtgggcga cctggtcctg gccgacgggg acggaatcgg cctggtagac gaggatgtcg
     2641 gaggccatga gcacgggata gccgaggaag ccgtaagtag tgaggtcctt gctggcgagg
     2701 ttctcctgct gttccttgta agtgggcacg cgctcaagcc acccaagagg ggtgatcatg
     2761 cccagcagca gcgggagctc gaaatgctgt ttgacctgac tctgcacgaa gagcacggac
     2821 ttcgcggggt cgaggccggc ggcgaggtag tcgaggatga cctcgcgggt attggcggcg
     2881 atttgcgaag ggtcggcgta atcagtggtg agggcgtgga cgtccgcaat gaaaaagtag
     2941 cactcgtact ggtcctgaag gcggacccag ttgtggaggg cgcccatgta gttgccaagg
     3001 tgaagtttgc cggtagggcg catgccggag aggatgcgct tgcggtcaga aacaagattg
     3061 gacacaaatt ccttcaagag ggaaatggct ctggttattg taacgggagc attgcatgca
     3121 gcagtccgcg cctttggcgg aaaacgctca aatctcgttc gcttgtcctg cgctgtaagc
     3181 taggagccgg gacgaactgt gaagacggcg atcagtgttt cgaaagagga ctacctcaaa
     3241 gcgattctgg aagccgagag cgaggggcag acggtcatct cggcgacgct ggcgcactgg
     3301 ctggcggtgt cggcgccagc ggtttcgatg gccctgcggc ggctgaagcg ggatgggtac
     3361 gtcgaggtgc gggcggatgg ggtggtgcgg ctgacgccaa aggggcacga gacggcgtat
     3421 cgaacggcgc tgcggcatca cctgatcgag cgcatgctgt cggagatgtt cggcatgccg
     3481 tggtacgagg tgcacgatga ggcggagcgc ctggagcatg cggtttcacc ggcgttcgaa
     3541 gcgctgctga tgaacaagct gggggagaag ggcacgtgtc cgcacgggaa cgcggtgctc
     3601 ccggagagcg atgcggcgcg gaagcgacat ggcgtgcggc cgctggcgga ggttgcggag
     3661 ggcatcgact acacggtaac cagcctttat gagcgcgacg agaaactgct gcgcttcctg
     3721 cagcaggcgg ggattgagcc gggcggctcg gtgcgggtgg tgcggcgcaa ctacgatcag
     3781 acggtgggaa tcgagacggc ggcggggcag ttcacaatcg ggccggcggc ggcggagaag
     3841 gtgcgggtga ggcgccgggg cggagctgga cgaaacggac actgatcgag taggtcaggg
     3901 ctgaagaaaa ccgaacaggc tgtgttgtac gcatgtagca ttttcacagt gcttccagtt
     3961 ctatccacac tgcgggctgc aggtgcgatg tgtgccttgt tgatggctct caccgcgctt
     4021 ccagcgcggg cgctcgatcc gcatacggca ctggcgcagt acggctacca gtcgtggcag
     4081 accgacacgg gactgccaca gaacacggtt catgcgattg tgcaggggcg cggtggcttc
     4141 ctgtggattg ccacggaggg cggtctggtg cgtttcgatg gggtggagtt caggacttac
     4201 acccgagcga atacgcggga cctgcccagc gatctgatcg acgatttgat ggaggacggc
     4261 gcggggacgc tctggatcgg cacgtctggt ggtttggcgc ggatgcggga tggcaagatt
     4321 gagtccttta attcggacaa gggaattccg gcgacccagg tgtggcggac attcgaggac
     4381 agaagcgggc gtgtgtgggc cctgacggcg tcagggctat ttaggattga ggggaatcac
     4441 gcgcagcgtg tcgcgctgga tgcggccctg acagagaaca gccgcatggt ggagggtacc
     4501 aacggatcgc tttggctcgg aacggcggat gggctgattc acggcaataa caagggagag
     4561 tttaggtcag ggcctaacca gggacaagaa gtggtggcgc tggcgacgga ttcggcgggg
     4621 cgtggctggg ccggaacgcg ctcaggactg gagatgtgta ccgaaggcgg cgattgtcat
     4681 cgtcctgcga ttccgcgcag tgccggggcg atccattcga tcgtgcggga tcgtcaggac
     4741 cgcatgtggg tgggaacgga cggcggcctg ttcgcgatta cgaatcggat cgtcggcgtt
     4801 gaagcaaccg ggcgcgtgga ttatctgtac gtcgatcggc agggaatggt gtgggccggg
     4861 accgcccacg gtttgatgcg ggtcgatccg gagaagccgg aaaccgcgga gtggctgaag
     4921 ggcggagatg tttttctcag cgcggctgag gatcgcgaag gcgacctgtg gctcggtacc
     4981 gaatcaggcg gcctggcggt ggtgcgcgat cgaaaattct cgacgctgac ggccgaggac
     5041 gggctgaccg acgaatacgt gctggcgctg gcgcagacgc ccaacgggca ggtctgggcc
     5101 ggcacgaagg gaggtggact gaacgttttt cgcgatggca aatttcacca ggtaacgacc
     5161 gagcagggcc tgtcgagcaa tgttgtgctg gccgtggcgt cggcgtcgcg cgcggatttg
     5221 tgggtgggaa ctccagatgg gctggacctg ttgcacgacg gcgagatggc tcgggtgttt
     5281 acgacggcgg acggtttggc ggatgacttt gtgcgctcat tgtttgtcga cagccatggc
     5341 gcgctgtgga tcgggactcg gcacgggctc tcgcggtatg taaacgggca gttcacgacc
     5401 tggacgacgc tggatgggct gggcagcgat ctggtcggcg cgatggcgga gagcaaggat
     5461 ggagtgctgt ggatcgggac gctcggggga ctgagccggt ttcgagaggg gaagttcaca
     5521 aacctgacag tgaaagatgg gctgtcgagc aacgtggtaa cggctctgca tgaggacagc
     5581 gacgagacac tgtggattgg gacgaacgat ggcgggctga atcgttggcg gaacgggaag
     5641 gttgtgagtc tggctgcggc cggaataccg caccgactga ccgggattct ggaggacggc
     5701 aggggatatc tgtggatcag ctcgaacagc ggggtttatc gtgcgagccg cgatgggctg
     5761 aatcgcatgg cagatggtgg gcctgcggtg gacgtgatgc ggtttggcac ggccgatggg
     5821 atgggtatca gcgagtgttc cagcggcgga cacccggcgg cgctgcggct gagggatggc
     5881 accttatggt ttgcgacgct gaaagggatt gcgcgcgtcg atccggtgca catgccgatg
     5941 aaccgggtgg cgcccgaggt gacgatcgaa caggtgagcg tggatgatgt gcaacagaag
     6001 gcggacgcaa agatcagcgt ttcgccggga cacagtcact acgaatttga ctacgccggg
     6061 ctgagctttg tggcgccgca gaaggtcgag taccggtacc agttggtggg gttcgatcgg
     6121 gactgggtgg atgcggggac gcggcgcgtg gcctattaca cgaatctgcc ccacgggcgc
     6181 tatacgtttc gcgtgattgc gcgcaacaac gatggtgtgt ggagcgaggc ggcggccacc
     6241 gcggggctga cggtggagcc gcacctttat cagacggtgt ggttccggct gctggtgctg
     6301 gcggcgattg cggcgcttgg gtacgccgcg tggaagcggc gactactgca tgtggagcgg
     6361 gagtttcagg cggtgctggg cgagcgtacg cgcattgcgc gggaaattca cgatacgctg
     6421 gctcaggggt ttgtcgctgt ctctgttcat ctggaacttg tagcgcagct gatgcggggt
     6481 tccgtggatg cagccaggga acagctggaa cgcgcgcagg cgctggtgcg aagcagtctg
     6541 gaagacgcgc gcacgtcaat ctgggagctg cgatcgcaga gcggggagcg ggaggatctg
     6601 gcggcgcgca ttttgaagac ggccgaagag gtgaccgggc gggcgcactc acatgcgcga
     6661 gtgcagatgc atgtgactgg aacgaatcat ccgctggacg aggatgtgga gcgagaactg
     6721 atgaggattg cgcgggaggc ggtgacgaac gctgttcggc acggcgatcc ggagaacatt
     6781 ggggtacggc tggagtttga ggcgggtatg ttcgggatgg agatacgcga tgatggccgc
     6841 gggtttaccg ggaccccggc ggacggagcc agcgggcact tcgggctcac gggcatgcga
     6901 gagcgggcgg aggcgattgg cgcaaacctg acggtggaga gcagtcccgg ccgtggcaca
     6961 agcgtgcgcg tagggttggt tctgaagggc gaaacggaga aacaaacgta actatggcgg
     7021 atacaattaa catcctgctg gtggacgacc atcatattgt gcgtcagggg ctggccgcgc
     7081 tgctgaaaac cgtgccggga ttcgcggtgg tggccgaggc atccgatgga gaacaggcgg
     7141 tggaggcttt ccggaaacac aagccggatg tgacgttgat ggatttgcgg ctgccgaagg
     7201 tgaatggggt agaggcgatt gggcgcatcc ggcaggaatt tccggcggcg cgcatcatcg
     7261 tgctgacgac tttcgatgga gacgaggaca tctaccgggc gatgcaggcg ggagcgaagg
     7321 gctacctgct gaagggcatg gatctgaatg agctgaccga tgcggtgaag gtcgttcatg
     7381 cgggcaagtc acgcattcct tccagagtgg ctgaaaagct ggcggagcga atgggcggcg
     7441 cctcacttac cgcgcgcgag ttagaggtac tgcaactgat cgtagcgggc aagagcaaca
     7501 aggagatcgg gaacgcgctg ttcatctcag aagcgacggt gaagacgcac gtgaacagtc
     7561 tgctcagcaa gatgggtgta gaggatcgta cgcaggcggc aaccacggcg ctgcagcgtg
     7621 gaatcgtgca tctggactga ggaacagggc ttagagcaca gagctcggag cgcaggaaaa
     7681 aaggatgggc gattgatgac ggttcatggc gggaagtggt tgactggtgc ggcgatcgtg
     7741 gtgatgctgg cgctttgcgc gtttgcgcag gagaagcgcg gttcggagtg gcgcgtggct
     7801 acagatgccg agctgcggtc ggtaattccg gcgcgggcgc cggtggtgca ggagcggatc
     7861 gaaacggagc tgcggtcggc ctcgggtatc acgaacggac acggaaaata catcgcgggc
     7921 gtggtgttaa ttacggcggg atactccgcc gaaggaaagt actcgcattt ctttctgacc
     7981 caggttccgg tacgggtggg caacgatact cggttggcgg ccggcgagta tgtgatcggg
     8041 tacgaacacg tagacaatgg tgaactgctg attcacttct atcaggcagc aactgggcgt
     8101 ccggtgggcg atgtggcggc gactctgttg accggaacca cgcgagtaga atcgtttcgc
     8161 atctggccgc cgggggagcg tgccgcaatt cagattgggc ggtttggctt cagctatcgg
     8221 atcgggagct agcaggcatg gcagagacgc tgtcgcaaaa agaacagaag gtggtggagg
     8281 cgctgggcga gaactggcag gcggagatgc gcggctttca cacctacaac acgcttgccg
     8341 agcgcgacga cgatccggtg cggaagaagg cgctgcggca catggccgag caggaggcgc
     8401 atcacgccgc gatgtgggca aagcggattc gcgaactggg tgcagagctg ccgaagtacg
     8461 aaggcagaga aaccggcgat gcggatacgc tggtgaaccg gctgggtgga ccgcagatgg
     8521 cgctgcggcg gctggagatc gaggagagcc gcgatatcgc gcgctacggg caacagctga
     8581 aggacctggg ggacgaaccg agcgtcgcga ttctgaaaca ggtgatcgcg gacgagcagg
     8641 aacattaccg cgagctgagc gagttggtgc gccaccggta tccgaaaacg gcaaatacgt
     8701 cggagatggc gcggcaggag ctggacgagt tgctgacgcg gcgcaatcgc tccgggcggc
     8761 agacggcggg ctggatcggc gacgcgattt acggcgtgaa cgatgggctg ggggcgattt
     8821 ttgggattgt atcgggcgta tcgggcgcga cgctgggcaa cagcaagtgg gtgctgctgt
     8881 cggggctggc ggggatgatc gccagcgcgc tgtcgatggg ctcgggcgcg tatctggcgg
     8941 cgaagagcga gcgcgaaatt tacgaggcag agctgacgcg cgagcgggaa gcgatcgaga
     9001 tgaacgggcc ggaggcgcgc gagctgctct cgctgtatta ccaggtgaag ggaattccgc
     9061 gggacgacgc ggatcatgtt gtggaacacc tggccgagtc gccgcagcag ttcacgcgag
     9121 cgctggcggc ggaacggctg aacatgaccg aagaaggact gagccagccg tgggtatcag
     9181 cgtggtcggg tgcgctctcg actgcggttg gggcattcat tcccatcgtg ccgttctttt
     9241 tcatgagcgg catcaacgcg gttctcgtat cagccattgt gtcactcatc gcgcattttg
     9301 ccgtgggtgc ggcaaagtcg ctgatcactg tacggagctg gtggtcgagc ggcctggaga
     9361 tgacgctggt gggggcgatt gaaggcgcgg cgacgtacgt cattgggatc gggcttggcc
     9421 acatcggggc atagacgcgt tcgatagcct ccattaccct cggacccatg gaaaattctg
     9481 cggtgctcct gatcgactgc ccggatcggc gcgggctcgt ggctggagtt tcgtcgctgc
     9541 tatacagaca cggggcgaac attcttcatg ccgatcagca ccgcgatgag gaactggggc
     9601 tgttcttcat gcgcgtggag tgggcacttg atgggaatcc tggaccgagt agcttcgaca
     9661 tggctgcttt cgagctggag tttgggtcga tggcggaagc gtttcaaatg cgctggagac
     9721 tgaccaccag cgaccaccgc acgcgcgtgg cgctgttctg ctctcactac ctgcattgtg
     9781 tggcggatct gttgcaccga tggaagagcg gggaactggc gtgcgacttc agcgcgattg
     9841 tttcgaacca tcgagaagca gaaccgctgg cgaacttcta cggcattccg ttcatgcatg
     9901 tggccgtcac tgcggcaaca cgcgaagccg gagaacagcg gcaactggcc cttctcgcgg
     9961 agaagaaagt ggagctggtt gtgctggcgc gctacatgca gattctgtcc gcgtcgttcg
    10021 tgagccaatt cccttcggca atcatcaacg tgcaccattc gtttctcccg gcgtttaccg
    10081 gggcgcggcc ttatcacgcg gctcacagcc ggggagtgaa gttgattggt gcgaccagcc
    10141 actacgtcac ggaagcgctg gacgaaggac cgattatcga gcaggacgta gcccggattt
    10201 cgcatcgcga tcaggtagaa gatctggtgg cgcggggccg cgatctggag cggatcgtgc
    10261 tctcacgcgc ggttcggtgg cacctggagc ggcggattct tcgctatggc aacaagacgg
    10321 tggtattcga ctgattttct ggatgccgca gtaggccgca aagcaaaggc cggagcactg
    10381 ggctccggcc tttgctttcg gaatgtcgct ggtcagtgga cgccgagccc atcgtgttcg
    10441 tggtagttcg ggtaggccgg tttgggatag gtcgggagcg gatgttcctt cagttgctcc
    10501 atgatgtatt gcgcggcgcg ttccagttga ggatcgtggc ctgcagcgac ttcctttggc
    10561 aggtattcga cctcgatatc gggtggaatg ccgtgattct cgacctcgaa ctggcctttt
    10621 aatccgtaga tcgccgtgcg aggtgcggtg atggtgccac catcgagcag gacagggtag
    10681 ccgccgatcc cgacgaggcc accccaggtg cggacgccga tgagtgggcc gaggcccgct
    10741 ttgcggaagt accagggcag gaggtctccg ccggaacccg cggactgatt gatgagcatt
    10801 gccttcgggc cgaagatggc gccctgggga tcggtgacat cctcgccttc ccgggtttca
    10861 tagcgcgaca tcggaacgcg gcgaagcgtg tcgatgacgt tgtcggcgat ggagccgccc
    10921 tcgttgtagc gctcatcgat gatgacgccc ttcttgtcga cctgggcgaa gaagtagcga
    10981 ttgaagttgg tgaagccgcc gccgccggtg ttgggcatat agacgtaggc gacctgatgg
    11041 tgggtcatct cgtcgacttt gcggcgggcg ctttcgatga aggcgagatt gcgcaggccg
    11101 tgttcggaat cgaccgcgac gacggtgaca tcgcgcgctc cggcgccgtc gggattgggg
    11161 cctacgcgca acacgacctg tttgccggcg gtgccttcaa agaagctgta aatattgtcg
    11221 gcggcatgga ggtcgcggcc gttcacagca aggagatact cgccctccct gacgttgacg
    11281 ccgggttggg tgagcggggc gtggagagcg gggttccagt tttccccgtt gtagatgtgg
    11341 gcgaacttgt agcggtcgtg atcgacggaa tagtcggcgc cgaggagacc ggttttgggg
    11401 gagtgatcgg gcgtgcgggg gccacggatg aacatatggc cgacggtgat ttcgccgagc
    11461 atctcctcga agaggtagtt cagttcgtcg cgggagccga tgccgtcaag gtagggccgg
    11521 tagcgctttt cgactttggc aatatcgagc ccgtggtgat tggggtcgta gaagaagtcg
    11581 cgttcgatgc gccaggtttc gttgtacatc tggcgatatt cggcgcgggg atcgacgtgg
    11641 gcctgaaggt tgccgaggtt gagggtcttg ccggggctgg tcttgacctc ctccgctgag
    11701 gcgatgagcc aggcgtcgcc cttcctgtag agcatctttt cgccattggc ggaaatggtg
    11761 taggcggaga tgccggaaag gacctcagtg gtttcgcgct tatccagagc atagcgccag
    11821 agtgcgtgaa tcggggcccc gttttgctgt gaaggattgg cgacggaact gccttcagcg
    11881 aggtagatca cgccttcctt gccggcttgg aggtcgatgt agttacgcgc cgggatgggc
    11941 agagcgagaa tgcgctgatc gatgccgtcg aaatcgactt tgaccacagg gggctttttc
    12001 tcgtccttct tttccttgcc agactcctcc ttcttttctc ctgcggcctt ctcgtcaggt
    12061 tttttctcgt cggccgcggg cttgttttct tcctttgcct tctcgtcatc gctctcgggg
    12121 ggcagcgggg agggcaggtc cttcgagagg acgacgatgt aggcattgct ggtgacggcg
    12181 cgatcgaggc tggagagatc gaaaccggca acggaggggc cgacgttcgt gctggcggtg
    12241 aagtagatat attttccgtt gcgatcgaag gcgagactgc cgatgtcgct cataccgtcg
    12301 gtaatctgat tggacttcag ggtatcgagg gagtagagga agacggcgtg catgtggttg
    12361 tggagctctt tgagatacgc aatccacttg ctgtcgggcg accagactga attgccggca
    12421 aagccgaagc cttcgaaatc ctgggtgtcg actttgaccg ggtgcgggct ctcaacgtcg
    12481 agataccaaa ggttgagttt cttgtcggcg tagaggatgt gcttcgagtc aggagaccag
    12541 cgaggcccgt agaagtatga aggcgctggg ccaaggtcga tggccttcgg cttcgcgatg
    12601 ccgtcctgat cgcggatgaa catctgatac tcgccggagg catcgctgaa atacgcgatg
    12661 gacttgccgt cggaagacca tgccggatcg cgctcggcgg cgttcgaggt gtcggtgaga
    12721 ttgcgggcgt cgcccttttc cgcggggacg gtgaagatat cgccgtgcgc ttcgaagacc
    12781 gcgcgctggc cggtgggtga gatggcggaa ttgaggatgg tatggggatc gatgacggcg
    12841 agtgatggtt cgagcgcagg aaggtcgccg tggagtgtga tctcaacggg gtgttgtgtt
    12901 ttcgtcgccg ggtcgtagag atggatgctg ccgaactgct cgtacacgat gccgccgggg
    12961 ccggcgccgg cggacttgag gtcgaagctc ttgttctcga tgacctgctc gacctgtttc
    13021 gattgggtgt cgtagctgaa gagggagacg gggccgttgc gatcggagag gaagtagact
    13081 ttgtctccga cccagagggg gtttttatcg ttggagttgt cgcgggggat tttggtgagg
    13141 ttgagggagg cgaggtcgac gatccagatg ggctcggtct ggccgccgtg gtagcgcttc
    13201 caggcgacct gccactgatt gaagggagtg taggcgatct gtttggcgtc gggggacagg
    13261 gaggcatcgt cgaccgaggg aagaggcaaa gcgacgggga gagccgatcc gtcggcagga
    13321 acgcggaaga gctgggcgaa gtcgttccag gcagcgcggc cgctgagaaa gagaatgtcc
    13381 ttgccgtcgg gcgaccagcc gacaacgcgg tcatcaccgg ggtggtatgt gaggcgatgg
    13441 ggaacgccgc ccgtcgcggg catggtgtag acgtcgatgt cgccgcgaat gcgtgcagac
    13501 caggcgaggg tctgaccatc cggagagaaa aaggggccgt caacgaccga agcggttgaa
    13561 gtgaggcggg tggcttcacc gccggcgcgc tggacgatcc agatgtcgtc ggcatggaca
    13621 aaggcgatct gatccttgct aagcgaagga tgcagcagga gcagaggggc ggcgtctctc
    13681 gcgcgagccg actcgggggt cgcggggaag ctcgacgcgt gggaagcgaa cggcaggacg
    13741 aggagcgcaa acagcacagc cgacgtgtgg cgaagcacag accctccagg atggcagaaa
    13801 tggtgaacag aaagtctacg cgcgttgttg caagcgcgct atgcgtttgg ggcgagaagt
    13861 gttgccgggt tctggggatc aggggttgcg gggatcgcgc aggcggaagc ggttggcgcg
    13921 gtcgaactca tcgcggagct gaggagtttt gaggttttcg cggatgtgag cgatgcgctg
    13981 ctcaagggcg tcgagggagc gggcaatggc gtcggagttg gtgtgggcga tgtcgcgcca
    14041 catggagaag gggctggcgc cgaggcgggt catctcgcgc agggcgcggc cgccgatggg
    14101 gagaaggtcg gcattggcgg cgaattcgtc ctcgaggagc gcggccaggg cggtggagac
    14161 gaactggggg agatgactga tccaggcgca gagcacgtcg tggcgggcgg ggtcgagatc
    14221 gatgatgcgg gcgccgaagc gggtgaccca ggtgcgccag tcctgcacaa ccggcgcacc
    14281 gccttctggg tgactccctg gagtgaacaa ccagactgcg tcctcgaaca gatcggcgtc
    14341 agcattttcc gcgccgctga cctctttgcc ggccatgggg tgaccgggga ggaagagggg
    14401 ctggccgggg ccgttgtatt tttcgcgggc atgttcgcag aggacgccct tgacgctgcc
    14461 gacgtcggtg acgagctggt cgtctcgcag aaagggggca aggcggtcaa gccattcgag
    14521 gatggtgaac acgggaccgc cgagaaggat aagactggct tcggctgcgg ccgcgagcgg
    14581 atcgtcggcg acgacggtga tggcctggcg gcggagggcg acatggacct ggcccgggtt
    14641 agggtcccat ccggcgatgg aaccggaaaa tccatgggca cgaagggcga ggcccaccga
    14701 cgagccgatg aggccggtgc cgaggatcgc gatgcgttcg atcatgagcg ggtacaggtt
    14761 acaactacag ggctcagggc tcagggcaca gagcttatat tgtgcgctct acggcggttg
    14821 cgatcactcg gagggagtcc atgagctttg cgaactggtc ggggaacatg gactgcgctc
    14881 cgtcggaaag ggccttatcg gggttggggt ggacctcaat cagcacgccg tcaccgcctg
    14941 cggctacggt ggcgcgcgcc atggcgggga ccttgtcgcg gcggccggtg ccgtgcgagg
    15001 gatcgccgac gatcggcagg tgggagagct tttttacgac ggggatggcg gagatatcca
    15061 tggtgttgcg ggtataggtt tcgaaggtgc ggatgccgcg ctcgcagagg atgacgtcgt
    15121 agttgccgcc ggcgagcaga tattcggcgc tgagcaggag ctcttcgatg gtggcggcga
    15181 tgccgcgctt gaggagcacc ggacggcgga cgtcgccgag ctcgcggagg aggttgaagt
    15241 tctgcatgtt gcgggcgccg acctggaaga ggtcgacgta gggcatcatg agctcgatct
    15301 gcgagatctc catgacctcg ctgacgacga ggaggccgtg ggcatcgcca gcttcgcgca
    15361 gcagcttgag ggcatcgagg ccgaggccat ggaaggagta gggcgagctg cggggcttga
    15421 aggcgccgcc gcggaggaat ttcgcgccgg cgtctttgac gagtttggcg caggcgaaga
    15481 tctgctccct gctttcgacc gagcagggac cggccatgac gacgacctcg tggccgccga
    15541 cgcagacgcc gttagggaag cggacgatgg tgccttcggg acggaagctg cgtccggcga
    15601 gcttgtacgg cgaggagatg cggatgactt ccgcgacgcc ggagaggacc tcaaattcgt
    15661 catggctgaa ggcgccgggg gtaccgacgc cggcgaggat ggtctggacg gtcccggtgg
    15721 tgcggtggac gttgaagccg atttccatca tgcgctcgac gacggcctga atttcttctt
    15781 cggtcgcgcg ctcctgcatg gcaacgatca ttggtcggtt cctgtctgtt ttgaatgggc
    15841 ttcgctggcg ggaatgccgg ggcgggaggc aagctcattg cgctgcaagg cgcgcatgac
    15901 gtcgacgatg cgctcgtaga tgtggacgag ctcaatgtcg gggagcgggc cgggattgtg
    15961 ggtgcggacg ttctcgaaga tgacgcgctc gcggttgggc tcgtagacgg ggaggtcggt
    16021 ggtgcgcttg aggcggccga tttcctgagc cgctgcggcg cgttcgctga tcagcagcac
    16081 gatctgccgg tcgagttcat cgattttgcg tcgccaggct gctatgtcca tggtgttcct
    16141 gatgatggag ggaaatggag agtgtccgca aaaaactatc gtatacggga gacaggcggt
    16201 cgggcttcgc gtccggcgtc gcgcgggcgg aagtaccgat tcaacgggcc ggcacggcgg
    16261 gctctttggc gacgccgcgc agaccctgga taaatcgggc gatggagttt gcggcttgct
    16321 gtgggccggc ctgttcgatg gtggcaacga gcgcgctgcc gatggcggcg gcgtcggcga
    16381 attcaccgac agtggcgaac tgttcggggg tggagatacc gaagccgacg gcgatgggga
    16441 gcctggtgaa gcgacgcagg cggcggacaa ggtgctcggc atcggaggcg agggattttt
    16501 gcgcgccggt gatgccggtg cgcgagacag cgtagacgaa tccctgggag acggcggcga
    16561 ttttttcgag gcgatcgtcg gggctggtgg gggcaaccag gaaaatcggg gcgaggcgat
    16621 ggcggtgcag tgcctgcagg tattcgccgg cttcttcaac gatcatgtcg atgagaagga
    16681 cgccgtcgat tccggcttgt tcggcagcgg cacagaaatt ctcaaggccg aagcgcagaa
    16741 cgggattgta gtaggtgaag atgatgaggc cggcgccggg gcgttcgagg cgtagatcgg
    16801 cagcgagggc gagcacgtcg tgaagacgcg tgccgttctt tacggcacgc tcgctggcgc
    16861 gctgg
//
LOCUS       DASZYN010000059         8442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_67210, whole genome shotgun sequence.
ACCESSION   DASZYN010000059 DASZYN010000000
VERSION     DASZYN010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67210"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /locus_tag="VHE33_01630"
     CDS             <1..1216
                     /locus_tag="VHE33_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HVT96175.1"
                     /translation="VVATIGVATAFFAATIGILQTDIKRVLAYSTISQLGYMFLGCGV
                     AAYSAGIFHLVTHAFFKALLFLAAGSVIHALSGEQDMRAMGGLRKKIPVTFWVMTAAV
                     LAISGIPPFAGFVSKDEILYQAFLSPNGGKIFWAVGLLTAGLTSFYMFRLWYMTFFGE
                     RRTPEAAHEEDLAAVHARSDTKPALEAEPHAHPVHESPWVMLAPLVILAILSVVGGFM
                     GWPEGLGGSNWFAHFLEPAVKTVHMTYEQASVALATGAASTPNVSPAGNKQLELTLAA
                     ISTLVAFLGWLLAYILYYAKPGLPARLAENLRALYSLVKNKYWVDEIYGALIVTPVLF
                     IARWVLGVLIDRVVIDGGTLTAGYSAQGLGALITRIQSGNIRSYAGWLALFAAALLVA
                     SYFGWTTHFFLR"
     gene            1235..2773
                     /locus_tag="VHE33_01635"
     CDS             1235..2773
                     /locus_tag="VHE33_01635"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HVT96176.1"
                     /translation="MLTDHILTLITFVPTAGAIVVAFLPRKGRVLPAFTLLVTLVTFV
                     LSLHLPAHFDYSNHGFQFEENQSWISTPAIRYHLGVDGLSLWLVLLATFLAPIGVLVS
                     WRAIQNRTKEFYFLFLLQQTAMIGVFVALDLFLYYGFWELSLVPMAILIALFGPTRGR
                     QAAIKFFLYTFIPSALFLVAILYLYAKAGTFDFVQLQSQLASGSLGLTPQALFWISLA
                     FLVAFAVKVPVFPLHGWLADVIQEAPTAMAMVVAGKLGLYSIFRFNLGLFPEQARAIA
                     PLMIVLAVVGLLYGALVALAQTDMKRLVAYSILGNLSFCTLGIFCFALAGLNGAVYQT
                     LNEGLTGGALLVLVSVLYERYGTNDMTLYGGLAGRLPWLATLFVITSLALVGLPLFNG
                     FVGEFLVLSGSFASHPRWVAVAALGVILSASYMLSMVQRIFYGRESSLVVNRPAPDLD
                     FREHITLWPMAILMLAMGVLSPYWIKAIEPAMTPLSGPVPAVTTPPSPIAAAAQLPGS
                     GGAQ"
     gene            2770..4281
                     /locus_tag="VHE33_01640"
     CDS             2770..4281
                     /locus_tag="VHE33_01640"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HVT96177.1"
                     /translation="MNPVTDFARIAPEVLFTLGGIVVMLADAVMPKASSRKSLGWLAV
                     LATLGALGVSFRQAQLPPGDAYYGVVRTDAFSAFFHVLIAGIVLATLLIILDAVTRET
                     EGLGELFALIQFGAVGMMLMSSAVELLLVFIALEISSISTYIMAGYRRKTGKSPESAL
                     KYFLLGSFATSFFLYGIALIFGATGTTRINDIAAAIANTSVATGQLHVLLYIGLAMVI
                     IGLGFKVSAAPFHVWTPDVYEGAPSPVVALMSTGPKAAAFAVLLRIVWGALPALQTHW
                     VPILWWLAVLSMTVGNLGALRQRNVKRMLAYSSIAHAGYILVAFTALAPEGIAAASFY
                     AVSYAAMNVGIFAIISHTGGYDDHLTLIDDYRGLAYASPLLAGSMAFFLISLIGIPFT
                     GGFFGKFYVFSAALHSGMVWLTIIGLINSGIAAYYYLRVVAAIYSQPTGAAPVTGVAR
                     PSFALLLAVFLTAATTLILGIVPGSVLSAASAAARTYPSAPASTTPPPATASR"
     gene            complement(4309..5091)
                     /locus_tag="VHE33_01645"
     CDS             complement(4309..5091)
                     /locus_tag="VHE33_01645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT96178.1"
                     /translation="MAGDKRVAVITGGAQGIGRQTAELLAERGYRIAIIDLQEPSAAV
                     AAIEARDADGFGFAGDITREEVVEAFSQRVLERFGRVDVLVNNAGISLIRAAEQTTAA
                     EYRRMMEVNLVAPFLLARVFGTKMLEARSGAVVNVASIAGLVAVADRAAYNASKHGLV
                     GLTRTLAAEWGGRGVRVNAVCPGWVKTEMDVADQKGGSYTDADITQRVPMGRFATPQD
                     VAKAIAFLADERESGFVNGATLTVDGGWNSDGSWESVRLRKR"
     gene            5198..5593
                     /locus_tag="VHE33_01650"
     CDS             5198..5593
                     /locus_tag="VHE33_01650"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVT96179.1"
                     /translation="MEIIRSGSQPSRSGPADYFTGTVRVDPLFDAPEPARVVGAHVTF
                     EPGARTAWHTHPLGQTLIVTSGCGRAQRWGGRIEEIHPGDIVWFAPGEKHWHGASPAT
                     AMSHIAIQEKLNGKTVEWLEHVTDEQYRA"
     gene            complement(5627..6835)
                     /locus_tag="VHE33_01655"
     CDS             complement(5627..6835)
                     /locus_tag="VHE33_01655"
                     /inference="COORDINATES: protein motif:HMM:NF006580.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HVT96180.1"
                     /translation="MTTNLSRRSFFGLAAAATAAAAVPILTEPELARAQRTRIVRDFP
                     PGSVRIDSNENPLGPCSGACATMSSLLPEGGRYNDELTVKFVQTFSAMEGLKPEYLRV
                     YAGSSEPLHYTVLAFTSKDRSLVVADPSYEAPMYAAQANGAKIVKVPLAKDYAHDVKA
                     MIAADPNAGVFYICNPNNPTGTVTSREDIQYALEHKPQGSVLLVDEAYLQFSDATPVL
                     DLVKADKDLIVLRTFSKIYGMAGLRCGLAIARPDLLAKLQVYGTNFLPMLAMAAATSS
                     LQVPELVPERKKINADIRNETFSWLTANNYKFIPSQTNCFLLDAGRPGRGVIDAMAEK
                     NVFIGRTWPIYPNHVRITVGTRSEMAKFQQAWKEVMNAPASASEPKRRGSPSLALGSG
                     DLPPAMRRLS"
     gene            7150..7587
                     /locus_tag="VHE33_01660"
     CDS             7150..7587
                     /locus_tag="VHE33_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVT96181.1"
                     /translation="MPNRIHVAFVAGLLATAAFGIAQTGPVVKQVPVRPTSAASGKQM
                     YNAYCAVCHGADGKGGGPAAVALKDVPSNLTQLAKDNGGKFPETHIYSVLQFGMEEPA
                     HGSKDMPVWGPALRSLDRGSPSTAMLEHQRLANLTNYLKTLQQ"
     gene            complement(7656..7964)
                     /locus_tag="VHE33_01665"
     CDS             complement(7656..7964)
                     /locus_tag="VHE33_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HVT96182.1"
                     /translation="MRKLQYLFMTLAALFMASPAFAQAAGGSSSSNSWVPIGAGIGMA
                     LASGLCGLGQGRAAGSAAEAVARNPGARAAIQLFLIFGLAFMESLALFTFVIILMKVK
                     "
     gene            complement(8083..>8442)
                     /locus_tag="VHE33_01670"
     CDS             complement(8083..>8442)
                     /locus_tag="VHE33_01670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HVT96183.1"
                     /translation="VPLGLAIFTFLYYHWHGLRAQGPIGYLKHFMGPVWWIAPLMFPI
                     EIISHFARILSLTVRLFANMLASDMITIIFFSMIPLALPIAGLGLHLFVALIQAYIFM
                     LLAMIYLGLATEHAESH"
BASE COUNT         1451 a         2968 c         2248 g         1775 t
ORIGIN      
        1 cgtcgtcgcg acgatcggcg tcgccaccgc cttctttgcc gctaccatcg gaattctgca
       61 gaccgacatc aagcgcgtcc tcgcctactc caccatctcg cagcttggat acatgttcct
      121 gggctgcggc gtggctgcct attcggccgg catctttcac ctcgtcacgc acgccttctt
      181 caaggcgctg ctcttcctcg cagccggctc tgtgatccat gcgctctccg gcgagcagga
      241 catgcgcgcc atgggcggcc ttcgcaaaaa gatcccggtc accttctggg tgatgaccgc
      301 tgcggtcctc gctatctccg gcattccgcc attcgctggc tttgtgagca aggacgagat
      361 cctctatcag gcctttctct cgcccaacgg cggcaaaatc ttctgggctg tcgggctgct
      421 caccgccgga ctcacctcct tttatatgtt ccggctgtgg tacatgacct tcttcggcga
      481 acgccgcacg cctgaagccg cgcacgaaga agacctcgcc gccgtccacg cccgttccga
      541 tacaaagccc gccctcgaag ccgagccgca cgcgcatccc gtccacgaga gcccctgggt
      601 catgctcgcc ccgctcgtca tcctcgcgat tctttccgtc gtcggcggtt tcatgggatg
      661 gcccgaaggc ctgggcggca gcaactggtt cgcccacttc ctcgagcccg ccgtcaagac
      721 cgtccacatg acatacgagc aggcaagcgt cgctctcgcc accggggccg ccagcacccc
      781 gaacgtctca ccggccggca acaagcagct tgaactgacc ctcgccgcca tctccacgct
      841 ggtcgccttt ctcggctggc tcctcgccta catcctttat tacgccaaac cgggacttcc
      901 cgcgcggctg gccgagaatc tccgcgcgct ctactcgctg gtgaagaaca aatactgggt
      961 cgacgaaatt tacggcgccc tcatcgtcac tcccgttctg ttcatcgccc gctgggtatt
     1021 gggcgtcttg atcgaccgcg tcgtgatcga cggtggcacc ttaacggccg ggtacagcgc
     1081 gcagggcctc ggagccctga tcacccgcat tcaatccggc aatattcgct cctacgcggg
     1141 atggctcgcc ctcttcgctg ccgccctgct cgttgcttct tatttcggat ggaccaccca
     1201 cttcttcctg cgataaagga cgaaagactc gaccgtgctg actgaccaca tcctgacgct
     1261 gatcaccttc gtccccactg cgggcgcgat cgtcgtcgcg tttctgccgc gcaaaggccg
     1321 cgtcctgccg gcctttaccc tcctcgtcac cctcgtgacc tttgtccttt cgctccacct
     1381 gcccgcgcac ttcgactact cgaaccatgg cttccagttc gaagagaatc agtcctggat
     1441 ctcgacgcct gccatccgtt atcacctcgg agttgacggc ctctcactct ggctcgttct
     1501 tctggccacc ttcctcgctc ccatcggcgt tcttgtctcg tggcgagcta tccagaaccg
     1561 caccaaagag ttttacttcc tctttctcct ccagcagacc gcgatgatcg gcgtgttcgt
     1621 cgcgctggat ctctttctct actacggttt ctgggaactt tcgctcgtgc ccatggcgat
     1681 tctcatcgcg ctcttcggcc ccactcgtgg acgccaggcg gcgatcaagt tcttcctcta
     1741 caccttcatc ccctcggcgc tgttcctggt cgcgatcctc tacctctacg ccaaagccgg
     1801 caccttcgac ttcgttcagc tgcagagcca gctcgcgtcc ggctccctcg gcctcacacc
     1861 gcaggctctg ttctggatat cgctggcttt ccttgtcgct ttcgcggtta aagtgcccgt
     1921 gtttccgctc catggatggc tcgccgacgt tatccaggag gcgcccactg caatggccat
     1981 ggtcgtcgcc ggcaagctcg gcctctactc catcttccgt ttcaacctgg gtctcttccc
     2041 ggagcaggcc cgcgccatcg cgccgctcat gatcgtcctt gctgttgtgg gtctcctcta
     2101 tggcgccctc gttgcccttg cgcagaccga catgaagcgc ctcgtcgctt actctatcct
     2161 cggcaatctc agtttctgca cgctcggcat cttttgcttc gccctggcgg gcctcaacgg
     2221 cgccgtctat cagaccctga acgagggact taccggcggc gcgctccttg tcctcgtcag
     2281 cgtcctttac gagcgctatg gcacgaacga tatgaccctc tacggtggac tcgccggccg
     2341 tctgccgtgg cttgctacgc ttttcgtcat cacctcgctg gctttagtcg gcctgccgct
     2401 gtttaacggc tttgtcgggg agttcctggt gctcagcggc tccttcgctt cccatccgcg
     2461 atgggtcgct gtcgccgcac tcggcgtcat ccttagcgcc tcatacatgc tctcgatggt
     2521 ccagcgcatc ttctacggcc gcgaatcgtc gctcgtcgtc aatcgccccg cgcccgacct
     2581 cgacttccgt gagcacatca cgctctggcc catggctatc ctcatgctcg cgatgggcgt
     2641 cctttcgcct tactggatca aggcgatcga gccggcgatg accccactct cgggtccggt
     2701 cccagccgtg accactcctc catctcccat tgccgccgcc gcgcaactcc cagggtcagg
     2761 aggtgcgcag tgaatccagt gaccgatttc gcgcgcatcg cacccgaagt cctcttcacc
     2821 cttggcggca tcgtcgtcat gctggccgat gccgtaatgc ccaaagcctc cagccgcaaa
     2881 tccctgggct ggctggccgt cctcgctacc ctgggcgcgc ttggtgtcag cttccggcag
     2941 gcgcagctgc cgcccggcga cgcttactac ggcgtcgttc gcaccgacgc cttcagcgcc
     3001 ttcttccatg tcctcatcgc cggcatcgtc ctcgccacgc tgctgatcat cctcgacgct
     3061 gttacgcggg aaaccgaagg cctcggcgaa ctcttcgcgc tcattcagtt cggcgccgtg
     3121 ggcatgatgc tcatgtcttc ggccgtcgaa ctcctgctgg tcttcatcgc gctcgagatt
     3181 tcctccatct cgacctacat catggcgggt taccggcgca aaaccggaaa aagccctgaa
     3241 tcggccctga agtactttct cctcggctcc tttgccacct cgtttttcct ctacggcatc
     3301 gcgctgatct tcggagccac cggcaccacc cgcatcaacg acatcgccgc ggcgatcgcg
     3361 aacaccagcg tcgcgactgg ccagcttcat gtcctcctct acatcggcct ggccatggtc
     3421 atcatcggcc tcgggtttaa agtctctgcc gcgccgttcc acgtctggac ccctgatgtt
     3481 tacgaaggcg caccttcacc tgtcgtcgcg ctgatgtcca ccggtcccaa ggccgcagcc
     3541 ttcgccgtcc tcctccgcat cgtctgggga gccctgcccg cccttcagac acactgggtg
     3601 cccatcctct ggtggctcgc ggttctctcc atgaccgtcg ggaacctcgg cgccctccgc
     3661 cagcgcaacg tcaaacggat gctggcgtac tcctcgatcg cgcacgcggg gtacatcctt
     3721 gtggccttca ccgcgctcgc gcccgagggc atcgccgccg ccagcttcta cgctgtctcc
     3781 tacgccgcca tgaatgtcgg catcttcgcc atcatcagcc acacgggcgg ctacgacgac
     3841 cacctcacgc tgatcgacga ctatcgtggc ctcgcgtacg cttcgccgct gctcgccggc
     3901 tccatggcct tcttcctgat ttcgctcatt ggcatcccgt tcactggcgg tttcttcggc
     3961 aagttctacg tattctccgc cgcgctgcac tccggaatgg tctggctcac catcattggg
     4021 ctcatcaaca gtggcatcgc ggcgtactac tacctgcgcg tcgtcgccgc catctattcc
     4081 cagccgaccg gggcagcgcc cgtcaccgga gtcgcacgtc cctcgttcgc gctgctgctc
     4141 gctgtcttcc tcaccgcggc gacgacgctg atccttggca tcgtccccgg cagcgtgctc
     4201 tcagctgcca gtgccgccgc acggacctat ccatccgcgc ccgcaagcac aaccccgccg
     4261 ccggctaccg cgtcccgata agcagcttcc ttcccattcc cggcttcgct acctctttct
     4321 caggcgtacc gattcccagc tcccgtccga gttccacccg ccgtcgaccg tcaatgttgc
     4381 accattcacg aatccgctct cacgctcatc cgccaggaac gcaattgcct tcgccacatc
     4441 ctgcggcgtc gcaaaacgcc ccatcggcac cctctgcgtg atgtccgcgt cggtatagct
     4501 cccgcccttc tgatcagcca cgtccatttc cgtcttcacc cagccaggac acaccgcatt
     4561 cacacgcacg ccgcgcccgc cccactccgc cgccagcgtt cgcgtcagcc caaccagccc
     4621 atgctttgaa gcgttgtacg ctgcgcgatc ggccacggcc accagccccg caatcgacgc
     4681 aacgttaacg acggccccgc tccgcgcctc cagcatcttc gtgccaaaca cccgcgccag
     4741 cagaaacggc gccacaaggt ttacctccat cattcgccga tactcggccg ccgtggtctg
     4801 ttccgcagcg cgaatcaggc taatccccgc gttgttcacc agcacatcca cgcgcccgaa
     4861 ccgttcgagc actctctgcg agaatgcctc cactacctct tccctggtga tgtctcccgc
     4921 gaatccgaat ccatccgcgt cgcgcgcctc aatcgccgcc accgccgcgc tcggttcctg
     4981 cagatcgatg atcgcgatcc gatagccccg ttcggcgagc aactccgccg tttgccgccc
     5041 gatcccctgg gctccccccg ttatcaccgc gactctttta tcccctgcca cgatccgcgc
     5101 tcctccgttg tgccctcagc ttaacaaccc ggctcgcgac ccttatcctg aatgggccac
     5161 atccaggccc gtttcgtcca ccagggagcc agccgccatg gaaattatcc gcagtggatc
     5221 tcaaccctca cgttcaggcc ccgctgacta cttcaccggc accgtacggg ttgatcctct
     5281 tttcgacgcg ccggaacccg ctcgcgtagt cggcgctcac gtcacctttg aacccggcgc
     5341 gcgcaccgcg tggcatacgc acccgctcgg ccagaccctt attgtcacct cgggctgcgg
     5401 tcgtgcgcag cgctggggcg gccgcatcga ggaaatccat cccggagata tcgtctggtt
     5461 tgctcccggc gaaaagcact ggcacggcgc atcgcccgcg acggcgatgt cccacatcgc
     5521 cattcaggaa aagctcaacg gcaaaaccgt cgagtggctg gagcacgtca cggacgaaca
     5581 gtaccgcgcc tgagcccggc ggcgttccag gaggcccagc ctgcggtcag gaaagccgcc
     5641 gcatggccgg cggtaaatct cccgatccca gcgccagcga gggcgaacct cttcgctttg
     5701 gttcggacgc cgaagccggt gcattcatca cttccttcca cgcctgctgg aacttcgcca
     5761 tctcgctccg ggttcccacg gtgatccgca cgtggttcgg atagatcggc caggttcggc
     5821 cgataaacac gttcttctcc gccatcgcgt cgatgacgcc tctgcccggt cgtcccgcat
     5881 ccagcaggaa gcagttcgtc tgtgaaggta tgaacttgta gttgttcgcc gtcagccacg
     5941 aaaatgtctc gttgcgaatg tccgcgttaa ttttcttccg ctccggcacc agttccggca
     6001 cctgcagact gctggtcgct gcggccattg ccagcatcgg caggaaattg gtcccgtaaa
     6061 cctggagctt cgccagcaaa tcagggcgcg cgattgcaag cccgcaccgc agacccgcca
     6121 tgccatagat cttcgagaag gtgcgcagca cgatcagatc cttgtccgct tttaccaggt
     6181 ccagcaccgg ggttgcatcg gaaaactgaa ggtacgcctc gtctaccagc aggactgacc
     6241 cctgcggttt gtgttcgagt gcatactgaa tgtcttcgcg gctggtcacc gtccccgttg
     6301 gattgttcgg attgcagatg tagaacacgc ccgcattcgg atcggcggca atcatcgcct
     6361 tcacatcatg cgcgtagtcc ttcgccaggg gcaccttgac aatctttgcg ccattggcct
     6421 gcgctgcata catcggggct tcgtagctgg gatcggccac caccagactc cggtccttcg
     6481 aagtgaacgc cagcaccgta tagtgcagag gctcgcttga tcccgcatat acgcgaaggt
     6541 attccggctt cagcccttcc atggcactga aggtctgtac aaacttcacg gtcaattcat
     6601 cgttatagcg accgccttcg ggcaaaagac tgctcatggt cgcgcaggcg ccgctgcatg
     6661 gtcccagggg gttctcgttt gagtcgatgc gcacggatcc gggcggaaag tcgcggacga
     6721 tgcgtgtgcg ttgcgcgcgc gccaactcgg gctcggtgag aatgggcacg gcggcagccg
     6781 cggttgcagc ggcggctaaa ccaaagaagc tgcggcgaga gagattcgtg gtcacctgtg
     6841 cctccctgag gtggagatta ggtccgaatg actttcccga atggcatcgt ggggaacgcg
     6901 atcattgtgc cacaatttgg ctgcgctttc ccaaaaagcc cattcccccg cgccaaatgg
     6961 agagaatgcc agaggcgcag ggcgcggttt gtatcggcca tcacattccc caaccgggat
     7021 cgctttcggc cacgattcac gaggctgtac acaacttttg gggtagttga aatcgccttc
     7081 ctgtgcccac catcacatac agttgtgcag gcaaaagtgg agcattaagt cccggaaagg
     7141 agggttatta tgccgaatcg aatccacgtc gcattcgtgg cggggctgct ggccaccgcc
     7201 gctttcggaa tagcccagac aggacctgtc gtcaaacagg taccggtacg gcccacatca
     7261 gccgcatccg ggaaacagat gtataacgca tattgtgccg tttgccatgg tgccgacggc
     7321 aagggcggcg gacctgccgc ggtagctctg aaagacgtac ccagcaacct gacgcagctt
     7381 gccaaagaca atggcggcaa gtttcccgag acccatatct attccgtatt gcagttcggc
     7441 atggaagaac cagcgcacgg ctcgaaggac atgcctgtat ggggtccggc acttcggtct
     7501 ctcgaccgcg gcagcccatc gaccgcaatg ctggaacacc agcggctcgc gaatctgacg
     7561 aactatctga agactctcca gcaatagggc tcttctccaa acaaaaaccc cctgccgcgg
     7621 tcccgcagca gggggttttt attgaacgat tcgatttact tcaccttcat caggatgatg
     7681 acgaaggtga acagtgccag cgattccatg aacgccagac cgaagatgag gaagagctgg
     7741 atcgccgcgc gcgctccagg gttgcgtgcc acggcttcgg cggccgatcc ggcagcgcgg
     7801 ccctgtccca gaccgcagag tccggaggcg agcgccatgc cgatgccggc gccgatcggc
     7861 acccagctgt tggaggagct gctaccgcca gccgcttgtg caaacgccgg ggaggccatg
     7921 aacagcgccg ccagggtcat gaagagatat tgcagtttcc gcattgtttc tcctgcttcc
     7981 gtaagattgc cgccagtggg tggttgaggc tcaccgtgct gaccccctca cgctgaacgg
     8041 ctttccattc cggagtggaa gcggtgctcc ggaaggaaat ctctaatggc tttctgcgtg
     8101 ctcggtcgcc aggcccagat aaatcatggc gagcagcatg aaaatgtaag cctgaatgag
     8161 cgcgacaaac aggtgcaaac ccagtccggc gatgggcagc gccagaggaa tcatcgagaa
     8221 gaagatgatg gtgatcatgt cgctggccag catgttggcg aagagacgaa ccgtgagcga
     8281 caggatgcgg gcaaagtgcg aaatgatctc aatggggaac atcagggggg cgatccacca
     8341 cacgggcccc atgaaatgct tcagatagcc aatcggaccc tgagctcgca gcccgtgcca
     8401 gtggtagtaa aggaacgtga agatggccag tcccagcgga ac
//
LOCUS       DASZYN010000060         1724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_195986, whole genome shotgun sequence.
ACCESSION   DASZYN010000060 DASZYN010000000
VERSION     DASZYN010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195986"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..241)
                     /locus_tag="VHE33_01675"
     CDS             complement(89..241)
                     /locus_tag="VHE33_01675"
                     /inference="COORDINATES: protein motif:HMM:NF023180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HVT96184.1"
                     /translation="MITILLIILILMAIGSVPAWPYSRGWGYYPSGGLGLILLIVVVL
                     LLLRVI"
     gene            complement(385..1095)
                     /locus_tag="VHE33_01680"
     CDS             complement(385..1095)
                     /locus_tag="VHE33_01680"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HVT96185.1"
                     /translation="MNLPELNERIVACERCSRLREHCTTVARLKRRAYREWEYWGRPV
                     PSFGDAHARVLFVGLAPGAHGSNRTGRPFTGDGSGDFLYPLLHQAGFASQASSRDRND
                     GMQLRDAWITAVVRCAPPGNKPLPGEIANCAPFLDEEIAHLSEVRVIVCLGKIGFDGV
                     VAHLLRTGQIDRRGELVFGHGVEYPIPGGRWLVGCYHPSLQNTNTGKLTAAMMLRVFR
                     RARKLAELPQAEPPLRVG"
     gene            1251..>1724
                     /locus_tag="VHE33_01685"
     CDS             1251..>1724
                     /locus_tag="VHE33_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96186.1"
                     /translation="MSNRTKLVIAPLALSLVAFLPGCKQSQPAPEATTQPAQPSVPQI
                     GGQPVVKITRPATSNAQGMEFLSATILPGRGMNLFQITANVPGKGEIPILASPTLEEA
                     AQALNGGPADDNGNASFSFGGAFLVPYPNRIVGPLSPDGKTVTTKWHGKTITLPAN"
BASE COUNT          353 a          599 c          454 g          318 t
ORIGIN      
        1 gtcagcagtt gaccgcatgt cgacgccgca tgcgcagaac gaaaagaggc acaggcatcg
       61 cctgtgccct ctcgtgcagc ttgtcctgct aaatgacccg caacagcaac agtacaacca
      121 caatcagcag gatcagtccc agtccgccac tcgggtagta gccccagccg cggctgtacg
      181 gccaggcagg aaccgagcca atggccatca ggatcaaaat gataagcaga atcgtaatca
      241 tgggtgtccc ccgtgcgcgg gagtcttatg cgctcccggg ctttaccaca agatgcgctc
      301 tgattacggc agggatgttt gcctcagcaa attaagaatt ccggtccgcg ggcaacctaa
      361 atggaactgc agacggatgc ttgctcaacc cacacggagt ggcggttccg cttgcggcaa
      421 ctctgccagt ttccgtgccc gccggaacac gcgcagcatc atcgccgctg tcagctttcc
      481 tgtattcgtg ttttgcagcg atgggtggta gcaccccacc agccaccgcc ctccgggaat
      541 gggatattcc accccatgcc cgaagaccag ttctccacga cggtcgatct gcccggtacg
      601 cagcaagtgt gccacgaccc cgtcgaagcc gatctttccc aggcacacga tcacgcgcac
      661 ttcgctcagg tgcgcgatct cttcatccag aaacggcgcg cagttcgcaa tctcccctgg
      721 cagcggtttg ttgcctggcg gcgcacaccg caccacggct gtgatccacg catctcgtag
      781 ttgcatccca tcgtttcgat ctcgcgacga tgcctgcgat gcaaatcccg cctgatgcag
      841 gagcggatac agaaaatctc ccgagccatc gccggtgaag ggccgccccg tccggttcga
      901 cccatgcgcg cctggagcca gtcccacaaa cagcacgcgc gcatgcgcat ctccgaacga
      961 cggcaccggc ctgccccagt actcccactc gcgatacgct cgccgcttga ggcgcgccac
     1021 cgtcgtacag tgctcgcgca gcctcgaaca gcgctcgcac gccacgatcc gctcattcag
     1081 ttcaggcagg ttcacgatcg tcagtttcga gcccggggtt gtatgttcgg taccagctgc
     1141 cacatcgccg attgtagtgg gcaacagcag cagacgccgc caggcgtaga atggtgccgc
     1201 ttcagaaagc gttttcgcgc agaaccgttt tcctaccgga gactcatcgc atgtcgaatc
     1261 gaacgaagct cgtgatcgcg cccctcgctt tgtccctcgt cgcctttctt cctggctgca
     1321 agcaatccca gcccgcgccc gaagctacaa cgcagccggc gcaaccaagc gttccgcaaa
     1381 ttggcggtca gccggtcgtc aaaatcaccc ggcccgccac cagcaatgcc caggggatgg
     1441 agtttctctc cgccacgatc cttcctggtc gcggcatgaa cctcttccag atcacggcca
     1501 acgtgcccgg caaaggtgag attcccatcc tcgcttcgcc caccctggag gaagccgccc
     1561 aggcgctcaa cggcggaccc gctgacgaca acggcaacgc cagcttctcc tttggcggcg
     1621 cttttcttgt tccttatccc aaccgcatcg tgggcccgct ctcacccgat ggaaaaacag
     1681 tcacgaccaa atggcatggc aagaccatca cgcttccagc gaac
//
LOCUS       DASZYN010000061         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_902426, whole genome shotgun sequence.
ACCESSION   DASZYN010000061 DASZYN010000000
VERSION     DASZYN010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902426"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /gene="rpsH"
                     /locus_tag="VHE33_01690"
     CDS             <1..310
                     /gene="rpsH"
                     /locus_tag="VHE33_01690"
                     /inference="COORDINATES: protein motif:HMM:NF001109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HVT96187.1"
                     /translation="KLKAEIARILKEEGYISNFKTTEENGRAVLRVYLKYGANNEAAI
                     RDLERVSRPGCRVYIGRDEIRRVQGGLGISILTTPRGVMTGRQARREGVGGELLCEVW
                     "
     gene            447..986
                     /gene="rplF"
                     /locus_tag="VHE33_01695"
     CDS             447..986
                     /gene="rplF"
                     /locus_tag="VHE33_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HVT96188.1"
                     /translation="MSRIGNKPIVLPQGVKYSFQGNVVTVEGPKGKMVQWIPAGINLE
                     QKDGHLHAVRENDSQAAIHGLARALVSNAVEGVTKGWTKDLDIVGIGYRAELKGKNTV
                     VFTLGYSHPIEFPLPTGITATIDPKQTKVTISGVNRQQVGQVAADMRSLRKPDPYKNK
                     GVRYSDERLKKKVGKTGAK"
     gene            1172..1528
                     /gene="rplR"
                     /locus_tag="VHE33_01700"
     CDS             1172..1528
                     /gene="rplR"
                     /locus_tag="VHE33_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HVT96189.1"
                     /translation="MIPQRKRNEIRQRVHTRIRRRLAGTEERPRLNIYRSLNHIYAQV
                     IDDAKGVTVVSASTVASKAKTGGNVAAAKEIGKLIAERAKEKGIKKVVFDRGGYLYHG
                     RVKALADAAREAGLEF"
     gene            1610..2119
                     /gene="rpsE"
                     /locus_tag="VHE33_01705"
     CDS             1610..2119
                     /gene="rpsE"
                     /locus_tag="VHE33_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HVT96190.1"
                     /translation="MATLRKKIDAGQYNLKDQVVAINRVTKVVKGGKNMSFAALVVVG
                     DPGAGVVGYGSGKAREVPQAIRKGIEAAKKNLVRVNLTQTTIPHQVLGRFGSGHVLLK
                     PAPEGTGVIAGGAVRAVMTSAGVQNVLTKSLGTANPHNVIKATFDALVQLRDRAEVAA
                     MRGKQVQEI"
BASE COUNT          539 a          628 c          732 g          350 t
ORIGIN      
        1 gaagctgaag gcggagatcg cccgcatcct caaggaagag ggctacatct cgaacttcaa
       61 aacgaccgaa gagaacggac gcgcggtgct gcgggtctat ctgaagtacg gtgccaacaa
      121 cgaagcggcc atccgtgacc tcgagcgcgt ttcgcgtccc ggctgccgcg tgtatatcgg
      181 ccgcgacgag attcgccgcg tgcagggcgg cctcggcatc agcattctca ccacgccccg
      241 cggcgtcatg accggacgcc aggcgcgcag ggaaggcgta ggcggcgaac tgctgtgcga
      301 agtttggtaa gaccagccaa tagctcctgg ctaacagctg ttagctaagc gctgtttgct
      361 aaacgctgcc ggacttcagt ccggcaccgg ctgcagtgga acggcaaggc accgccggga
      421 ctcgcaagcc acgaaggatt tgaacgatgt cgcgaattgg aaacaaaccg attgtgttgc
      481 cccagggcgt caagtattcg ttccagggaa acgtcgtcac cgtcgaagga ccgaagggca
      541 agatggtgca gtggattccc gccggcatca acctcgagca gaaggacggt catctgcacg
      601 cggtgcgcga aaacgactcc caggccgcaa tccacggact ggcgcgcgcg ctggtttcga
      661 acgccgtgga aggcgtgacg aagggctgga cgaaggatct ggacatcgtc ggcatcggct
      721 atcgcgcgga actgaagggc aagaacaccg tcgtgttcac cctcggctac tcgcacccca
      781 tcgagtttcc gctgcccacc gggatcaccg cgaccatcga tccgaagcag acaaaggtca
      841 cgatcagcgg cgtcaatcgc cagcaggtag gacaggtggc ggccgatatg cgttccctgc
      901 gcaagccgga tccgtataag aacaagggcg tccgctactc cgatgagcgc ctgaagaaga
      961 aggtcggcaa gacaggagcg aagtaaggca gagtttagaa atcagagttt ggagtttagg
     1021 agggccgcag tcaggggccc gctaaagggc aagagttaga aggttggtta agtccgagtc
     1081 gacctaaacg ctaaccgcta aacgctaacc gctgcagtta aaaccgaacg gggccgcagg
     1141 gcggctccgc cattaggaag gaagaacaaa gatgattccg cagaggaaaa gaaacgagat
     1201 tcgtcagcgc gtgcacacgc gcatccggcg ccggctggcc gggactgagg agcggccacg
     1261 gctcaacatt taccgctcgc tgaaccacat ctacgcgcag gttatcgacg acgcaaaagg
     1321 tgtcacggtc gtctcggcct cgaccgtggc ctccaaagcg aaaaccggcg gcaatgtggc
     1381 ggcagccaaa gagatcggca aactgatcgc cgagcgcgcg aaagagaagg gcattaagaa
     1441 ggtggtcttc gaccgcggcg gctatctgta tcacgggcgc gtcaaagcgc tggcggatgc
     1501 ggcaagagaa gcagggcttg agttctaaaa gcagcgcaga gtgtagagcg gataagtttg
     1561 ccttttatac gctgaacgct cgacgctaaa cgctgtactg aaggaaaata tggcgacatt
     1621 aagaaagaaa atcgatgcgg gacagtacaa cctgaaggat caggtcgtgg cgatcaaccg
     1681 cgtcaccaag gtcgtaaagg gcggcaagaa catgtccttt gcggcgctgg tggtggtggg
     1741 cgatccaggc gcgggcgttg tgggctacgg ctcgggcaag gcgcgcgagg ttccgcaggc
     1801 gatccgcaaa gggatcgagg cggcgaagaa gaacctcgtc cgcgtgaacc tgacccagac
     1861 caccattccg catcaggtac tggggcggtt cgggtccggc catgttctgc tcaagccggc
     1921 gccggaaggc actggcgtga ttgccggcgg cgcggtacgc gcggtcatga cctcggcggg
     1981 cgtgcagaac gtgctcacca agagcctcgg caccgccaat ccgcataacg tgatcaaggc
     2041 gaccttcgat gcgctggtgc agctgcgcga tcgcgccgaa gtcgccgcaa tgcgcggcaa
     2101 gcaggtgcag gagatctaac catggcgcag aagaaagcag cagcgaaggc ggcggaaaag
     2161 gcgaccatcc agattcagta cttccggtcg atgatccagg cgcccgtcaa acacaagaag
     2221 gtcattaagg gcctgggctt cacccggct
//
LOCUS       DASZYN010000062         5565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_965082, whole genome shotgun sequence.
ACCESSION   DASZYN010000062 DASZYN010000000
VERSION     DASZYN010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965082"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VHE33_01710"
     CDS             <1..193
                     /locus_tag="VHE33_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96191.1"
                     /translation="DPNGAGLPNWPKYSGASDALMDFTNNGPVAGPDPLKQRLDLAEQ
                     LATKPAASTESNDVKAQPK"
     gene            226..2493
                     /locus_tag="VHE33_01715"
     CDS             226..2493
                     /locus_tag="VHE33_01715"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HVT96192.1"
                     /translation="MNARKIGSVAIGAAATFVLLAVTHAAQAQLGPQKQEPKGPWADK
                     TLSPDRRADLVIEKMTLDEKIQLLHGQGWKSLFTPAESGSGVRSLGGAGFVPGIPRLG
                     IPDVQMSDAAVGVARGAQKSRYSTALPSGTALASSWDPKVAYEYGTLIGTELRDQGFN
                     MSLGGGVNLTREPRNGRTFEYEGEDPILAGTMVGQMMKAEQAQHVIGDIKHYAVNDQE
                     DGRNYVNSIIDERALRESDLLAFQIGIRDSDVGAVMCSYNMINGTYACENEHTLRDVL
                     DHDWKFPGFVVSDWGGTHSTVKAAKAGLDIEMPGDDYFGAALKKAVQSGQVSQQLLNE
                     MVHRVLRTEFATGIIDDPVKTKVVDVFHGLHVAQHVEEQGLVLLRNEDHQLPLDASAI
                     HSIAVIGGHADAGVMSGGGSGQVDPAGGNAVAPPPGSPTGLEALLSEQVYHRSVPLEA
                     IRHRAMKAKVEFNAGTDPAAAAALAKSSDVAIVFAVEHESEGSDLPNLSLPNDQDALI
                     EAVAAANPHTIVVLETGGAVTMPWIDHVSAVLETWFPGIRGAQATTAILFGDVNPSGK
                     LPLTFPRSEADLPHPVLTHQPKGPMTPMPGMPDWKVNTKTFSMKYDEGLKVGYKWYDA
                     ENKTPLFPFGFGLSYTTFAYSDLKVEPADALQVSFTVRNTGERAGEEIAEVYAAFPAD
                     AQEPPRRLIGWQKVALGPGEEKTVTLHVEPLYLSIYDVSSKGWRLVPGQYTISAGGSS
                     RDLPLTSSVTLAEQH"
     gene            2603..3898
                     /locus_tag="VHE33_01720"
     CDS             2603..3898
                     /locus_tag="VHE33_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96193.1"
                     /translation="MASAAQNAGPRLRWLFLDLNSYFASVEQELRPELRGRPIAVVPV
                     MADTTCAIAASYEAKAFGVRTGTQIGEAKRMCPGLELVEARHEVYVDYHNRIVEAVER
                     CVPVAAVMSIDEMACSLIGREQPLFAAMDLARRVKASIRENVGSTLRCSVGLAPNRYL
                     AKIASDMDKPDGLVALTQDLLPGALLHLTLRDLPGVGARMEKRLHEQGIRTMDALLAL
                     DHEQMNHVWGGVGGEKLWHWLRGEDFHDAELEHQKSISQSHVLPPELRTEEGCYAVAH
                     KLLHKAAMRLRAARLWATHVTLTIKYATSKAEAKGQHSSGIHQTAWSQGLAVIECQDN
                     QTLIETLQKLWAARPQDEKHRKPFFIGVWLGELVPDHLHTLSLFAGLETETKRTRLTS
                     TMDALNQKYGTSTLFPASMLLARAAAPTRIAFTSIPDLF"
     gene            4015..>5565
                     /locus_tag="VHE33_01725"
     CDS             4015..>5565
                     /locus_tag="VHE33_01725"
                     /inference="COORDINATES: protein motif:HMM:NF004952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HVT96194.1"
                     /translation="MDHENFPAPVESSNETPEPTTEIPSESAAEVSSILEAAVPLPPE
                     APPTPAEPVESFAELLSEFEQEHKRPAENRQVEATVISVSADTVYLDIGFKVEGVLPR
                     AAFENNADEVKPGDKVHVSIKSRNAEGYYELSRFKVKQPTDWTSLQAAFAEKATIAGT
                     VTGVVKGGLTVDVGVRAFLPASRSGARDAKEMGELVGQSVEVRITKVDEADEDVVVDR
                     RVVLEEQALTAQASRWSSLQPGEVLEGKVRSLMSYGAFVDLSGIDGLLHISDMAWSRV
                     EKPEDLLSVGQEIRVKVLKIDPETRRISLGLKQLEPEPWETAGNKYKAGERVRGTVTR
                     LTDFGAFVELEPGIEGLIHVSEMSWVKKIHKPSDVLKQGDVVEAVILGVQPAERRMSL
                     GLKQALGDPWADAARKWPAGAAVEGPVTRLTKFGAFVQAAEGVEGLVHISEIVADRRL
                     NHPSDVLKVGQVVRAQVLGVDAEKRQMKLSMKQLIPTSIDEYVAEHKVGDVVSGRVVE
                     EGDGRAIVE"
BASE COUNT         1083 a         1448 c         2057 g          977 t
ORIGIN      
        1 ggatccaaac ggagcgggct taccgaactg gccgaaatac agcggcgcca gcgatgccct
       61 gatggacttc accaacaacg ggcccgtcgc cggccccgat ccgttgaagc agcggctgga
      121 tctggcggag caactcgcga caaaacctgc cgcgagcacg gagtcgaacg acgtcaaggc
      181 ccaaccgaaa tagcgggaca accgaaatcc aggggaagtt caacgatgaa cgcacgaaag
      241 atcgggagcg tcgccattgg agcggccgcc acctttgtcc tccttgctgt gacccacgca
      301 gcacaggcgc aattgggacc gcagaagcag gaaccgaaag gtccgtgggc ggacaagact
      361 ctttcaccgg atcggcgcgc cgatctggtg attgaaaaga tgacgctgga cgagaagatc
      421 cagttgctgc atgggcaggg gtggaagtcg ctgtttacgc cggcggagtc gggatcgggg
      481 gtgcgttcgc tcggaggcgc gggctttgtt cccggcattc cacgcctggg cattccggat
      541 gtacagatgt cggatgccgc ggtgggggtg gcgcgcggag cacagaagag ccggtattcg
      601 acggcgctgc cgtccggaac ggcgcttgcg tcgagctggg atccgaaggt cgcgtatgag
      661 tatggaacgc tgattggcac cgagctgcgc gatcagggat tcaacatgtc gctgggcggc
      721 ggcgtgaacc tgacgcgcga gccgcgcaac gggcgcacgt tcgagtacga gggcgaggat
      781 ccgatcctgg ccggcaccat ggtgggccag atgatgaagg ccgaacaggc gcagcacgtg
      841 atcggcgaca tcaagcacta cgcggtcaac gatcaggagg atgggcgcaa ctatgtcaat
      901 tcgatcattg atgagcgcgc gctgagggag agtgacctgc tggcgtttca gatcggcatt
      961 cgggattcgg acgtgggcgc ggtgatgtgc tcctacaaca tgatcaacgg aacgtacgcc
     1021 tgcgagaacg agcacacact gcgggatgtg ctggatcacg actggaagtt tcccgggttt
     1081 gtggtttcgg actggggtgg cacgcacagt acggtaaaag cggcgaaggc gggcctcgat
     1141 atcgagatgc cgggcgacga ttattttggc gcggcgctga agaaggcggt gcagagcggg
     1201 caggtatccc agcagttgct taatgagatg gtgcaccggg ttctgcggac ggaatttgcg
     1261 acgggcatca tcgacgatcc ggtgaagacg aaggtggtgg acgtatttca cggcctgcat
     1321 gtggcgcagc acgtggagga acagggcttg gtactgctgc gcaatgagga tcatcagcta
     1381 ccgctggacg cgagcgcgat tcactccatt gctgtgatcg ggggccatgc ggatgcgggc
     1441 gtgatgtcgg gtggcggatc agggcaggtc gatcctgccg ggggcaatgc tgtggcgcct
     1501 ccgccgggaa gcccgaccgg actggaggct ctgctgagcg agcaggtcta tcaccgctcg
     1561 gttccgctgg aggcgattcg gcatcgcgcg atgaaggcga aggtggagtt caatgcgggc
     1621 accgatccag cggcggcggc ggcgctggcc aaatcttccg atgtggcgat tgtgttcgcg
     1681 gtggagcacg aatccgaggg cagcgatctg cccaaccttt cgctgccgaa cgatcaggac
     1741 gcgttgattg aggcggtggc ggcggccaat ccgcatacga ttgtcgtgct cgaaaccggc
     1801 ggcgcggtga cgatgccgtg gatcgatcac gtgagcgcgg tgctggagac atggttcccg
     1861 gggattcgcg gcgcgcaggc aacgacggcg attttgttcg gcgatgtgaa tccgtcgggg
     1921 aaactgccgc tgactttccc cagaagtgaa gcagatttgc cgcatccggt gttgacgcat
     1981 cagccgaagg gaccgatgac gccgatgccg gggatgcccg actggaaggt caacacgaag
     2041 accttcagca tgaaatacga cgaagggctg aaggtcggct acaagtggta cgacgcggag
     2101 aacaagacac cgctgttccc gtttgggttc gggctgtcgt acacgacgtt cgcttattca
     2161 gatctgaagg tggaaccggc ggatgcgctg caggtgagct tcacggtgcg caataccggc
     2221 gagcgggcgg gggaggagat tgccgaggtg tacgcggcat tccccgccga tgcgcaggag
     2281 ccgccgcgac gcctgatcgg atggcaaaag gttgcgctgg ggccgggcga agagaagacg
     2341 gtgacgctgc acgtggagcc gctctatctc tcgatttacg acgtcagcag taaaggatgg
     2401 cggctggtgc cgggccagta cacgatttcg gccggtggat cgtcgcggga tttgccgttg
     2461 acaagcagcg tgacgctggc cgagcagcac taaaaaagtt cacaactcgc tatgggcgat
     2521 cctcccgggt cgcccttttt ttgcttcaga gcgggccgga gagcggtctt gcagagaggc
     2581 gaagagaaga cgaaaatggg gcgtggcttc tgctgcccaa aacgccggac ctcggctgcg
     2641 ctggcttttt ctcgatctga attcctactt cgcttcggtg gagcaagagt tgcgaccgga
     2701 actgcgcggg cgtccgattg cggtggtgcc ggtgatggcc gataccacgt gcgccattgc
     2761 tgcgagctat gaggcgaagg ccttcggagt ccgcaccggc acgcagatcg gcgaagcgaa
     2821 gcggatgtgt ccggggctgg agctggtgga ggctcggcac gaggtctacg tggactacca
     2881 caaccggatt gtggaagcgg tggagcgctg cgttccggtg gcggcggtga tgtcgatcga
     2941 cgagatggcg tgcagcctga tcgggcgcga gcagccgctg tttgccgcga tggaccttgc
     3001 gcgtcgcgtc aaggcttcga ttcgcgagaa tgtcggctcc acgctgcgct gttctgtggg
     3061 gctggcgccg aaccgctacc tggcgaagat cgcgtccgat atggacaagc cggacggtct
     3121 ggtggcgctg acgcaggatc tgctgcctgg agcgcttttg cacctgacct tgcgtgatct
     3181 tcctggagtg ggcgcgcgca tggagaagcg cctgcacgag cagggcatcc gcacgatgga
     3241 tgcgctgctg gctctcgatc acgagcagat gaaccacgtg tggggcgggg tcggcggcga
     3301 gaaactctgg cactggctgc gcggcgagga ttttcacgat gccgagctgg aacatcaaaa
     3361 atcgatcagc cagagtcacg tgctgccgcc cgagctgcgc acggaagagg gctgctatgc
     3421 ggtggcgcac aagctgctgc acaaggcggc gatgcgtctg cgggccgcgc ggctgtgggc
     3481 gacgcatgtc acgctgacga tcaagtatgc gacgagcaaa gctgaggcga agggccagca
     3541 ttcgtcgggg attcatcaaa cggcgtggtc gcaggggctg gcggtgattg aatgccagga
     3601 caaccagacg ctgattgaga cgctgcaaaa actgtgggcg gcgcgaccgc aggatgagaa
     3661 gcatcgcaag ccgttcttca tcggcgtgtg gctgggagag ctggtgccgg atcatctgca
     3721 cacgctgagt ctgtttgcgg ggctcgagac agagacgaag cggacgcggc tgacgagcac
     3781 gatggacgcg ctcaatcaga agtacgggac gtcgacgctg tttccggcga gcatgctgct
     3841 ggcgcgggcg gcggcgccga cgcggattgc gttcaccagc attccggatt tgttctgagg
     3901 gaccggggac tggggactgg ccgtccaggc agatgtgctt cgcaccgttc cagtcaacgg
     3961 cgggcttgag ggggtcaggt ggggagaggc gtgaaagaat gaaagcaaca cgccatggat
     4021 cacgagaact tccctgcccc tgttgagtcg tcgaacgaga cgccggaacc caccacagaa
     4081 attccttcgg aatcggcggc tgaagtgtcc tccattctgg aggccgcggt gccgctgccg
     4141 ccggaggcgc ctccgacacc cgcggaacct gttgaatcgt tcgccgaact gctgtccgag
     4201 ttcgagcagg aacacaagag accggcggag aaccgccagg tggaggcgac cgtcatttcg
     4261 gtttcggcgg acacggtcta tctcgatatt ggattcaagg tggaaggtgt gttgccgcgc
     4321 gccgcgtttg agaacaacgc cgacgaggtg aagccgggcg acaaggtgca tgtctcgatc
     4381 aagagccgca acgctgaggg ctactacgag ctgtcgcgtt tcaaagtgaa gcagccgacg
     4441 gattggacgt cgctgcaggc ggcctttgcg gagaaggcga cgattgcggg aacggtgacg
     4501 ggcgtggtga agggcggtct cacggtggac gtgggcgtgc gcgcgtttct tccggcttcg
     4561 cgcagtggcg cgcgcgacgc gaaggagatg ggcgagctgg tgggccagtc ggtcgaggtg
     4621 cgcatcacca aagtcgacga agccgatgaa gacgtggtgg tggatcggcg cgtggtgctc
     4681 gaggagcagg cgcttaccgc gcaggccagc cgctggtcgt cgctgcagcc gggcgaggtg
     4741 ctcgaaggca aggtgcgcag cctgatgagc tatggcgcgt ttgtggattt gagtgggatt
     4801 gacgggctgc tgcacatcag cgatatggcg tggagccgcg tggagaagcc ggaggatctg
     4861 ctgagcgtcg gccaggagat tcgcgttaag gtgctgaaga tcgatccgga aacacgccgc
     4921 atttcactcg ggctgaagca gctggagccg gagccttggg agacggcggg caacaaatat
     4981 aaggctggcg agcgcgtgcg gggcacggtg acgcggctga cggatttcgg cgcgtttgtg
     5041 gaactggagc ctggcatcga aggcctgatc cacgtgtcgg aaatgtcgtg ggtgaagaag
     5101 attcacaaac ccagcgatgt gctgaaacag ggcgatgtgg ttgaggccgt gattctcggc
     5161 gtgcagccgg cggaacggcg catgtcgctc gggttgaagc aggcgttggg cgatccgtgg
     5221 gcggacgcgg cgcggaagtg gccggctggt gcggcggttg aaggtccggt gacgcgactg
     5281 acgaagtttg gcgcgtttgt gcaggccgct gagggcgtcg aggggctggt tcatatttcg
     5341 gagatcgtgg cggaccggcg gctgaatcat ccgagcgacg tgctgaaggt ggggcaggtg
     5401 gtgcgcgcgc aggtgctggg cgtagatgcg gaaaagcggc agatgaagct gagcatgaag
     5461 cagctgatcc cgaccagcat cgacgagtat gtggccgagc acaaggttgg cgatgtggtc
     5521 tccgggcgcg tggtggaaga gggagacgga cgggcgattg tcgag
//
LOCUS       DASZYN010000063         3660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_386962, whole genome shotgun sequence.
ACCESSION   DASZYN010000063 DASZYN010000000
VERSION     DASZYN010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386962"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VHE33_01730"
     CDS             complement(<1..295)
                     /locus_tag="VHE33_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96195.1"
                     /translation="MPLLLVLEDNPPDRRVAAQLGSSAGFDEFELSGSATDIKAYLAK
                     ALEGKVPLPDAMLIDLSLGYDSGFEILRFWRSTPRLKPIPVVVWTLARDTQLEI"
     gene            complement(437..1159)
                     /gene="bshB1"
                     /locus_tag="VHE33_01735"
     CDS             complement(437..1159)
                     /gene="bshB1"
                     /locus_tag="VHE33_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HVT96196.1"
                     /translation="MAETPIADVLAIAAHRDDVEQTCGGTLLRMRAIGVSTAILDLTR
                     GEAGTRGSANDRATEAAEAARILGVGWREALDIPDGRVENTWENRLKIVEALRRVRPR
                     VVILPYWTGRHPDHYNTATLGYEACFLSGLAKIETGTAPHRPFKIVYAALYADVRPSF
                     VVDITPFIEERHRALMAYQSQYANQAAGSGLFVPEEEIRERTFAEARHYGTLAGVRYA
                     EPFVQKEVGLVNDLTQVPVQSL"
     gene            complement(1186..2676)
                     /locus_tag="VHE33_01740"
     CDS             complement(1186..2676)
                     /locus_tag="VHE33_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96197.1"
                     /translation="MKKRSPLYFVAVLGTVFVVSTVISSCGKNFFFAGRSLPPSGVLN
                     RVMIAEQNPSAFATGALPFMDAYYDIRHAFNASTGQLEINGYSGKLPLTIQNMPEQQV
                     GAVYGAGDGSLTLINYAQEKVEAPVTIPGGLSSGTEGVPYNGVFISRDLGYVYAANPS
                     SHVISVVDRVAGSALALNLPNAYGISVNPGGTVALVFVENATQAAGHTVNVDNPATFA
                     VYSVVHLNQTQQTAAANNPNYLGAQDCEPQRQPTWCVFPVSTGASATFDHPVKAVFSP
                     DGTTAYVVNCGPECGGTTASLTTIPITASAINSGSTGASGIGLVAQSNVLIPNGATNA
                     IFNGNTLYVAGQQFQTSSSLFAGFLTVVNTPANTIAGTYSIGDGMHNRMVFADDGTLW
                     IGSSQCNQGVRYQQAQAGGNIQFGCITMFNTGSHAATIDSYKGDGTGIADIEGLHKVY
                     TTEGGQIYIYKTTDMSELDNFNVTIAGTAIDCAYMDSGTDNNNTIY"
     gene            2827..>3660
                     /locus_tag="VHE33_01745"
     CDS             2827..>3660
                     /locus_tag="VHE33_01745"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HVT96198.1"
                     /translation="MSPTTNRLEQRFSGRTVLCDGAMGTSLYARGVFINRCYDELNLS
                     QPELVRSVHEDYLQSGAEIIETNTFGANAIRLQRYGLRDKVADINRAGVAIARQAVRQ
                     LAEKQAGTAYVAGSVGPLGVHLEPLGKTSLEEAKEAFAEQIRALVAGGPGVGVDLLII
                     ETMPALNEAEQAVLAAKEVAPEIPVVVLVTVDEEANCLDGASPETAAARIELWGVDAI
                     GCNCSVGPATVLTAVERMAAVTSLPLMAMPNAGMPRSVEGRNIYLCSPEYMASFARKF
                     TK"
BASE COUNT          715 a         1020 c         1208 g          717 t
ORIGIN      
        1 agatttcaag ctgggtgtcc cgggccaggg tccagacgac gacggggatg ggcttgagac
       61 gcggagtgct ccgccagaag cggagaatct caaatccact gtcgtagccg agggagagat
      121 cgatcagcat ggcatccggt agaggaacct tgccttccag agccttcgcc agataggcct
      181 tgatatcggt tgcggagccg ctcagttcga actcgtcgaa tccagcgctg ctgcccagct
      241 gggccgctac gcggcggtcg ggaggattgt cttcaagaac gagcagaaga ggcatacaga
      301 aacccggggg ggtgaggaca gtttacggca ggaacagcgg ttcgccactt ggctctttcg
      361 cgtcatggac ttccacagga acgaacaggc atcagcgacg aggcggatga tcggtcatca
      421 acctgagaat ccgccgctaa agtgactgga cgggaacttg ggtcaagtcg ttgacgaggc
      481 cgacttcttt ctggacgaaa ggttcggcat agcgcacgcc ggccagggtt ccgtagtggc
      541 gggcttcggc aaaggtacgc tcgcgaattt cttcctcggg aacgaacagt ccgctgccgg
      601 cagcctggtt ggcgtattgc gactggtatg ccatcagagc acgatgccgt tcttcgatga
      661 acggtgtgat gtcgaccacg aagctcggcc gcacatcggc ataaagcgcg gcatagacaa
      721 tcttgaatgg acggtgcggt gcggttccgg tttcgatttt tgccagtccg gacaggaagc
      781 acgcttcata gcccagagtc gccgtgttgt agtggtcggg atggcggccg gtccagtagg
      841 gaagaatgac gacgcgcggt cgcacgcggc gaagcgcctc gacgattttc aggcggttct
      901 cccaggtgtt ttccacgcgg ccgtcgggga tgtcgagggc ctcacgccag ccgactccga
      961 gaatgcgcgc ggcttcggcg gcctcggtcg cgcggtcgtt tgcggatccg cgtgtgccgg
     1021 cttctccacg ggtcagatcg aggatcgccg tggagacgcc gatggcccgc atgcggagta
     1081 aagtcccccc acacgtctgt tccacgtcgt cgcggtgagc ggcgatggcc agaacatcgg
     1141 cgatcggtgt ctctgccatg cctcaagccg gcgcgggccg gctcctcagt agatggtgtt
     1201 gttgttatcc gtgccggagt ccatgtacgc gcagtcgatc gctgttccgg cgatggtcac
     1261 gttgaaattg tccagctcgg acatgtcggt ggtcttgtaa atgtaaatct ggccaccctc
     1321 ggtggtgtag accttgtgca gaccctcgat gtcggcgatc ccggtgccgt cgcctttgta
     1381 ggaatcgatg gtcgcggcat gggagccggt attgaacatg gtgatgcagc cgaactggat
     1441 attgccgcca gcctgagcct gctggtagcg cacgccctgg ttgcactgcg aggagccaat
     1501 ccacaaggtg ccgtcatcgg cgaagaccat gcggttgtgc atcccatcgc cgattgaata
     1561 ggttccggcg atggtattgg cgggggtatt cacaacggta agaaaacccg cgaacaggct
     1621 actggaagtc tggaattgct ggccggcaac atagagcgtg ttgccgttga agatggcgtt
     1681 ggtggctcca ttcgggatga ggacattgct ttgcgcaacg agaccgatgc cgctggcgcc
     1741 ggtgctgccg gaattgattg cagaagcggt gatcgggatc gtcgtcagac tggccgtcgt
     1801 gccgccgcat tccggtccac agttcaccac ataggcggtg gtgccatccg gagagaaaac
     1861 ggctttgacg gggtgatcga aggtggcgga agcccctgta ctgaccggaa acacgcacca
     1921 cgtgggttgc cgctgcggct cgcagtcctg tgcgccgaga taattcggat tgttcgccgc
     1981 ggcagtctgc tgtgtctgat tcagatggac gacgctatac acggcgaagg tcgcaggatt
     2041 gtcgacattg accgtgtgtc cggcggcctg ggtggcgttc tcgacgaaca caagagcaac
     2101 ggttccaccg gggttgacgg aaattccata ggcgttgggc agattcagcg ccagagctga
     2161 accggcgacg cggtcgacca cgctgatcac atgactggaa ggattcgcgg cgtagacata
     2221 gcccagatcg cggctgatga agactccgtt ataaggaaca ccttccgtgc cgctggagag
     2281 gccgccggga attgtcaccg gcgcttccac tttctcctgg gcgtagttga tcagcgtcag
     2341 gctgccatca ccggcgccgt agacggcgcc cacttgctgt tcgggcatgt tctgaatggt
     2401 cagcggcagc ttgccggaat agccgttgat ctccagttgt cccgtggaag cgttgaaggc
     2461 atgccgaatg tcgtagtacg cgtccatgaa gggcagcgca ccggtcgcga acgcgctggg
     2521 gttctgctcg gcgatcatca cgcgattcag aacgccgctg gggggcagac tgcggcccgc
     2581 aaagaagaaa ttctttccgc aggagctgat cacggtcgaa accacgaaga cagtaccgag
     2641 gacggccacg aagtacaggg gacttctctt cttcaaaact ttagaacctc accaggcgct
     2701 tggcttcaag acaatctcgc gggaaacccc aagtatatca gggcgcttgc ggcaatttgt
     2761 tggacgcggg gattgcggct gggtgaggtc ctcaggccga tattccgcct gatacgatga
     2821 atcagaatga gccccacgac gaaccgtctg gagcagcgat tcagcgggcg caccgtcctg
     2881 tgcgacggcg ccatgggtac ctctctgtac gcacgagggg tcttcatcaa tcgttgctac
     2941 gacgaactga acctctccca gcctgaactg gtccgcagtg tgcacgagga ttacctgcag
     3001 tccggtgccg agatcatcga aaccaacacg ttcggcgcga atgcgatccg cctgcagagg
     3061 tatggactgc gggacaaggt ggccgatatt aatcgcgccg gcgttgctat cgcccgccag
     3121 gccgtccgcc agcttgccga aaagcaggct gggacggcgt acgtcgcggg ttcggttggg
     3181 ccgctcggcg tgcatctgga accgctgggc aagacgagcc tcgaagaagc caaagaagcc
     3241 ttcgcagagc agattcgtgc gctggtggcc ggcgggcccg gggtgggcgt ggatctgctg
     3301 atcatcgaaa ccatgccggc cctgaatgaa gccgagcagg cggtcctggc tgcgaaggaa
     3361 gttgccccgg agattccggt ggtggttctg gtgaccgtcg atgaggaagc gaactgcctc
     3421 gacggagcct cgcccgagac cgctgcggcg cgcattgaat tgtggggagt cgacgccatc
     3481 ggctgcaact gcagcgtggg accggcgacg gtgcttaccg cggtcgagcg catggctgcg
     3541 gtgacgtctt tgccgttaat ggcgatgccg aacgccggca tgccgcgctc ggtcgaagga
     3601 cgtaacatct atctttgttc gccggagtac atggccagct tcgcccgcaa attcaccaaa
//
LOCUS       DASZYN010000064         3067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_838649, whole genome shotgun sequence.
ACCESSION   DASZYN010000064 DASZYN010000000
VERSION     DASZYN010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838649"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="VHE33_01750"
     CDS             complement(<1..997)
                     /locus_tag="VHE33_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HVT96199.1"
                     /translation="MKTGAPKPNVTMLATAGMVAYACGALLQHASPDARWFGIGLRVV
                     ALLVLAFAAARRRSLTGWIFVAMLAGIELGVDAPHAALSMRVFGDIFLRLIRVIVAPL
                     IFGTLVTGIAGHAEAKTVGRLGLKSLVYFEALTTIALFIGLVAIDVSKCGVGLNMSAH
                     VAVAPGTLPTQTTISWQQFLLHVFPENLASAVAENQILQVAVFAIFFGLALCKVPEAK
                     RAPMLRLCESLTEVMFAFTNYVMLYAPVGVAAALAYTVAQSGLGVMLNLGKLALTLYI
                     SIALFALLAMLPALLLARLPLREFLRAVAEPATIAFATSTSEAALPRAIEAMEAYG"
     gene            1113..1658
                     /locus_tag="VHE33_01755"
     CDS             1113..1658
                     /locus_tag="VHE33_01755"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVT96200.1"
                     /translation="MRSLVWSANIVMWIAISGGLAGGQLLQPTAPQPGATPTGSVLTN
                     PGLTPQDQFLYDLEAKFAADTAKGGGKAFGSWFAPDAVTLANGEAPVLGHDAIAARAT
                     WTPEAYQLRWTPEGARMSPGGDMGFTWGHYEGDSKDAQGHPVKKTGRYMTVWKKQPDG
                     SWKVEMDASNEGPPEDCCRLK"
     gene            1925..>3067
                     /locus_tag="VHE33_01760"
     CDS             1925..>3067
                     /locus_tag="VHE33_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVT96201.1"
                     /translation="MSYLASSAQWSEADRLKSLHEYEILDTPADPAFDEIVRVAAQVT
                     GAPYAYLGFLDSNRLWFKSRFGFQGTEIPRLATADQYTILEPEPLLVADAAMEPRFPV
                     RGIPLTRTVHCRSYLAAPLVGPEGGIGTLAVLSPKPNQFTEQHASILSVLARQIMMRL
                     DYSIHSRGQDHSLRARQRIERALMVERNFVSAVLDTMSALVLVVDTAGRIVRFNRACE
                     EISGYTFAELAGRSFPQELFLAGERANAFQLLEESRSDLRREPTELHWLTREGRRRLI
                     AWTTTTLTDGSGGINFIIMTGVDVTDQREATSALYASEARYRQLVESSLGMVCTHDLE
                     GRILSINASAAESLGYRVDEMEGRRLAEFVPDEHRTVFENYLRRIAV"
BASE COUNT          625 a         1064 c          877 g          501 t
ORIGIN      
        1 cgccgtacgc ctccatcgcc tcaatcgccc gaggcagcgc ggcttcactg gtgctcgtcg
       61 caaacgcaat cgtcgccggc tccgccaccg ctcgcaaaaa ctccctcagc ggcagccgcg
      121 ccagcagaag cgccggcagc atggccagca aggcaaagag cgcgatcgaa atgtacagcg
      181 tgagcgcgag tttccctagg ttcagcatca cgcccagccc cgactgcgcc accgtgtacg
      241 ccagcgccgc cgccacgccc accggcgcgt acagcatcac gtaattcgtg aacgcgaaca
      301 tcacttccgt cagcgactcg cacagccgca gcatcggcgc ccgcttcgcc tcgggcacct
      361 tgcacagagc cagcccgaag aagatcgcaa acaccgccac ctgcaggatc tggttctctg
      421 ccaccgcgct ggcaaggttc tccggaaaca catgcagcaa aaattgctgc cacgaaatcg
      481 ttgtttgcgt tggcagcgtc cccggcgcaa ccgccacgtg cgccgacatg ttcagtccca
      541 caccgcactt cgacacgtca atcgcgacca gaccgatgaa cagagcaatc gtcgtcagcg
      601 cttcgaagta aaccagcgac ttcagcccca gccgcccgac ggttttcgcc tccgcatggc
      661 ctgcgatgcc cgtgaccagc gtcccaaaaa tgagcggagc gacgatcacg cggatcagcc
      721 gcagaaagat gtcgccgaag acgcgcatcg aaagggccgc atgcggtgca tcgacaccca
      781 attcgatccc ggcgagcatg gccacgaaga tccaccccgt gagcgaccgc cgtcgtgcag
      841 cggcgaacgc aaggaccagc aatgccacga cgcgcagtcc gatcccgaac caacgggcgt
      901 ccggactggc gtgctgcagc aacgcgccac aggcgtaggc caccatcccc gcggtcgcga
      961 gcatcgtcac attcggtttg ggcgcgccgg tcttcatgcg agaacagcaa gaatatctga
     1021 gacgacagcg tacagggaca gcgaaggaga aacgcgctcg aacagcgaat caggaatctc
     1081 accgttgggg cgcgctggtc taaacttttt ccatgcggtc tctggtttgg tcggcgaaca
     1141 tagtgatgtg gatagcgatc tctggcggct tggcaggcgg gcagctcctg caacccaccg
     1201 ctccgcaacc cggcgccacc cccaccggaa gcgtcctcac caatccgggt ctcacgcccc
     1261 aggaccagtt cctctacgac ctcgaggcaa agtttgcagc cgatactgcc aaaggcggag
     1321 gcaaggcctt cggatcctgg ttcgctcccg acgccgtaac cctcgccaac ggtgaagccc
     1381 ctgtcctcgg acatgacgcc atcgccgcac gcgcgacctg gacgcccgaa gcctaccagc
     1441 tgcgctggac ccccgaaggc gcccgcatga gccccggcgg agacatgggc ttcacctggg
     1501 gccactatga gggcgattcc aaagacgccc agggacatcc cgtgaagaaa accggccgct
     1561 acatgaccgt ctggaagaag caacccgatg gaagctggaa ggtcgaaatg gacgcaagta
     1621 acgaggggcc gccagaggat tgctgccgcc tgaagtgacc ggatttggta ccaaaactcc
     1681 ggtcgcaacc cggctttatg ttaccgtagt gccttagcaa agtgggtcac gccgttacca
     1741 ccggaaagta ccgtaagcga tacatatcgc aggacttggc atcacccaaa cggggaagag
     1801 ccgaggcccg tctggtctgc aagaatgctc ctgaaccccc acacgcagtg tcctggcgtg
     1861 agaccttcac gccgcacacc gatgtgcccg gggacctcgg atccgcaagg atctgtggag
     1921 agggatgagc tatctggcaa gcagtgcgca gtggagtgaa gcggaccggc taaagtcgct
     1981 gcacgagtac gaaattctcg atacgcccgc cgacccggcg ttcgacgaaa tcgttcgcgt
     2041 cgcagcgcag gtcaccggcg cgccttacgc ctatctcggc tttctcgact cgaatcgcct
     2101 gtggttcaag tcgcgtttcg ggttccaggg aaccgaaatt ccgcgccttg ccaccgcgga
     2161 tcaatacacc atcctcgaac cggagccact tctggtcgcc gatgcggcca tggaaccgcg
     2221 ttttcccgtc cgcggcatcc cgctcacgcg gaccgtgcac tgccgctcgt atcttgccgc
     2281 gcccctcgtg gggccggaag gcggcatcgg caccctcgcc gtcctctcgc ccaaaccgaa
     2341 tcagttcacc gaacaacatg cctcgatcct gagcgtgctc gcccgccaga tcatgatgcg
     2401 cctcgactat tcgattcact cgcgcgggca ggaccattcc ctccgggcgc gccagcgcat
     2461 cgagcgcgcc ctcatggtcg agcgcaactt cgtctccgcc gtgctcgaca ccatgagcgc
     2521 tttggtgctg gtcgtcgata ccgccggccg catcgtgcga ttcaatcgcg cctgcgaaga
     2581 gatttccggc tatacctttg ccgagcttgc cggccgctcc ttcccccagg agttatttct
     2641 tgccggcgag cgcgcaaatg ccttccagct tcttgaggag tcgcgcagcg acctgcgccg
     2701 cgagcccacc gaactccatt ggctcacgcg cgaaggacgc cgccgcctga ttgcctggac
     2761 tacgacgacc ctcaccgacg gctccggcgg gatcaatttc atcatcatga ccggcgtcga
     2821 cgtcaccgat cagcgcgagg ccacctcggc gctctacgcc agcgaagcgc gctaccgcca
     2881 gctggtggaa agctcgctcg gcatggtctg tacccacgac ctggaaggcc gcatcctctc
     2941 catcaatgcc agcgctgcgg agagtctggg ctatcgcgtg gacgagatgg aaggccggcg
     3001 tctggcagaa tttgtgcccg acgagcacag gacggtcttt gagaattacc tgcgccgcat
     3061 cgccgtc
//
LOCUS       DASZYN010000065         9033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_196186, whole genome shotgun sequence.
ACCESSION   DASZYN010000065 DASZYN010000000
VERSION     DASZYN010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 9033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9033
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196186"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..1357
                     /locus_tag="VHE33_01765"
     CDS             311..1357
                     /locus_tag="VHE33_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96202.1"
                     /translation="MSFRTQLRSRVFCLAAIAIPFTLFVRADGRCQSILPGPGDSVPA
                     PMIPQPLQVFSQVPLSTPPFGYLRTAQCDDSGTMFFAASTPPRTGVTYLSLSADGQRQ
                     TVYPMPKYVDENPWNTLFSVPPDGKLQVLFVLPGQQPPRWISFDKDGKITRVVTLSAP
                     SDIDVRSFAATSDGSLLLLGFYPLTKAHGKDEGNTYTAIFNRNGDLVTRLTPEDAGIQ
                     PNGQFAGSPEEPAAVEGDQFFSISSSGKSLEVIATDGSVVRRLPLPAVRPGDQLVGLN
                     ISGNLALMTYMNFKATPRSSYLLLNAATGEPYRFFLPPAAVQGSLTCFDSSRGFTFLS
                     GGNGHLSLIQSALP"
     gene            complement(1377..1787)
                     /locus_tag="VHE33_01770"
     CDS             complement(1377..1787)
                     /locus_tag="VHE33_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96203.1"
                     /translation="MIDIGAMGPGSMVFVFLAVGAVALFSMISVATWSGARQKEREAY
                     YKNDMLKKLADAQGPGANSALELMREEARLSTLRTRQGLQIGGLVTAAAGLGVLIFLR
                     ALLGSEQGVWLCGMIPLLVGMALFGSSYLVKTAE"
     gene            1947..2591
                     /locus_tag="VHE33_01775"
     CDS             1947..2591
                     /locus_tag="VHE33_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HVT96204.1"
                     /translation="MNPDPARPESSDEALNDQAPNDEALVARVVAGNTAAFEGIVRRW
                     QSPLVNLAWRFCRDRGRAEEMAQEAFLRAFRNLAQFRGDSAFSTWLFALATNLYRTEI
                     RRLPSMPLSLDEIAEPPDPRIAAATALEEADRGESIRRAVLALPPKYRDAVLLFYFHE
                     QDVPAAARTLRIPEGTVKARLFRGRELLRAKFSRARVSKSTGLASDSSTEVRTL"
     gene            2588..2986
                     /locus_tag="VHE33_01780"
     CDS             2588..2986
                     /locus_tag="VHE33_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96205.1"
                     /translation="MKTRTHRPPLTDADLDRALAPASDSILPSSGFAASVMAAVHEEQ
                     AAPAPIPFPWKRAVPGLLAVLAALAILIVAVPAALRSAMAAAQQHQAAGSIQGSLTAL
                     AHPGNPTTWISASVILSLACLLFCRKLIAR"
     gene            3145..4764
                     /locus_tag="VHE33_01785"
     CDS             3145..4764
                     /locus_tag="VHE33_01785"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HVT96206.1"
                     /translation="MSTTIAGPVSFVLTDGATRRILTITHSPFTIGRLPENDLVLAHP
                     FVSRRHADLILEGDRAFIADQASRHGTFVNGQPTPGRHAIGASDTIHFGALDGPSLRL
                     STDHASADFPSGTSTIRNIIEQIPGVGAGNPLDKLRWFFESARKLNTSDAVDQILTAL
                     LDTTLQLTQVERGYVFLRKETGEMELALGRDNKGEALTDQSTLSHGAMRQAVSTSNEF
                     IVTDTLSADSDTRSASMVAHSIRSVICIPLRKRRPGAARETDLLGLLYLDSRLKPGML
                     TEIDNDLLRTISTDAAALIDNAQLAVSEERERVYRQEMNIAADIQRGLMTVRPPTLTW
                     AKVAARSVPCRECGGDFFDIIEDGEALAVVVADISGKGISAAILGATLQGLMYSLLVA
                     RQPLATIAEIVNRYICGKKVEKYATMAVLRISPNGHMEYINCGHVPPFLYSGGCTRLS
                     EGNLPVGLIEGAPFTSGTLQLQPGARVLIVTDGVTEAADLGGEFYGEPRLTAPEFESL
                     TLDEIFSDVEKFAGSLAADDDCTVMDVRYLG"
     gene            complement(4795..6732)
                     /gene="asnB"
                     /locus_tag="VHE33_01790"
     CDS             complement(4795..6732)
                     /gene="asnB"
                     /locus_tag="VHE33_01790"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HVT96207.1"
                     /translation="MCGIAGLLCLKNSTQVDVPIVLAGMSATMESRGPDASGQWIGPD
                     DRMGFAHRRLSIIDLDVRADQPMHSGDGRYTIVFNGEIYNYQELREELKAKGRRFRTT
                     SDTEVLLAMYAECGAAMLNRLRGMYAFAIWDQEAEELFLARDPFGIKPLYYAEGGGRF
                     YFASQVRALIGVPDVDLQDEPAGHVGFFVWGSVPEPYTLYRGIRCMPSGTWMRASGHG
                     ISPPKRFASPVDEALQFDAGAMPTSQEEANVILHDALRESLRMHEIADVPVAIFLSAG
                     IDSGIIASLAGESGNDVEALTLGFDRLRGTAEDETDDAAEVAGHYGLPHYTRFVDQSI
                     FAEHRDRLLEQMDQPTVDGVNTYFISWLARERGFKVALSGLGGDELFGGYPSFHQIPK
                     LVKGLRPLRRTPWVGRGVRVVTQGMISRFTSPKYASLFEYGGSWGGAYLLRRGLFMPW
                     ELPGFLDADLVREGWRKLGAIEEMNALVQPFGNLRNVAKEQADFLRVSALETRYYMRR
                     QLLRDADWAGMAHSVEIRVPLVDMVLLRQISALRASRFSPRKPGMVQCLRKPLPAKIL
                     NRPKSGFVVPVREWLETGELDSGGAKAGGHFSDRGLRSWAAHVHRVLTRRSGFLSGSA
                     QTSSGLVETSMVIGKPGSRGR"
     gene            7019..8191
                     /locus_tag="VHE33_01795"
     CDS             7019..8191
                     /locus_tag="VHE33_01795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT96208.1"
                     /translation="MKILHVIGSLSPADGGPPEAVRQLARAYLQIGVEVEVLCQDAPA
                     ASFLAELSVPVHALGQRWLGRFGLSPRLWRWLHRNASRFDGIVMNGLWIFPDLAVRSA
                     ARRAGIPYAVFAHGALDPWFNTRYPLKHLKKRLYWPLQYRVLRDARAVLFTTPTERDL
                     ALTSFQPNQWNSLPVPYGIGEPTGDPSAQVELFYRQVPELRGRRFLLFMSRLHAKKGC
                     DLLVEAFARVAAGHPDVDLVIAGPDQQGFQAQLQRTCAEKGIGDRVFWPGMIRGDLKW
                     GALHAAEAFILPSHQENFGIVVVEALAAGTPVLTTNKVNIWKEIQEDGAALIEDDTLE
                     GTGRLLHRWLRFSPAERQTMAAQAQPCFRARYSMRETAIVIRDLFAQARPKSRASI"
     gene            complement(8265..>9033)
                     /locus_tag="VHE33_01800"
     CDS             complement(8265..>9033)
                     /locus_tag="VHE33_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HVT96209.1"
                     /translation="PVGEIDRSPFQTRSRFDEVQLAELAASITATGVVQPIVVRPLPH
                     GRFQLIAGERRWLASQRAGKETIPAIVRQVSDEQAMEMTIVENLQRSDLNPMEQARAY
                     ERLAREFQLTQEQMAKRTGKDRASVGNFLRLLKLPTEVQTKVEEGVLSFGHARTLLPL
                     EDPQAIQKAAQKVVALSMSVRQTESYVQKLLTPAPAADGEQKEKPVDPNVREVQESLQ
                     RALGLKVRIEDKKGRGRVIIEYGQLEEFDRLLERLQR"
BASE COUNT         1661 a         3432 c         2268 g         1672 t
ORIGIN      
        1 cccggccgtg ccttttcgcc aacacccagc gcacactcct tccccgccgg caatccattc
       61 cccagccctg cgagagatcg ccctcagccg ctccggctcc ggatgtctcg tttatcggcg
      121 gaaatcaaag gaaaatgaca acgtgcccgg ctctccttcc ggccgcgccg cccacactct
      181 tcctgacaca ccaccctgag cccctgaagc gctgcttatc acgcaggtga cgtttttccc
      241 gccccccggt cttttgcctc gcaggggtat tctcgggttg ggttttccag cccacggggt
      301 tcggatccgg atgagcttca gaacacagct ccgcagccgt gttttctgcc tggcggcgat
      361 cgccatcccc ttcactctgt ttgtccgagc cgacgggcgc tgccaatcca tcctgccggg
      421 ccccggcgat tcagtccccg caccgatgat tccccagccc ctccaggtct tttctcaggt
      481 gcccctctcg acgccgccct ttggatatct gcgcaccgcc cagtgcgacg actccggcac
      541 catgttcttt gccgcatcga cgccgccgcg aaccggcgtc acgtacctct ccctctccgc
      601 cgatgggcaa cgtcaaaccg tctacccgat gccgaaatat gtcgacgaga acccctggaa
      661 cacgctcttc tcggtgccgc ccgacggaaa gctccaggtg ctcttcgtcc tgcccgggca
      721 gcaacctccc cggtggatct ccttcgataa ggatggaaag atcaccaggg ttgtgacctt
      781 gtccgctccg tccgatatcg atgtccgaag ttttgcagcc acgtccgacg gctctctgct
      841 cctgctgggt ttctatccgc tgaccaaggc gcacggaaaa gacgaaggga acacgtacac
      901 ggccatcttc aaccggaatg gcgacctggt gactcggctg acgcccgaag acgccgggat
      961 ccagccgaac ggccagttcg cggggtctcc cgaagaaccg gctgcggtcg aaggcgatca
     1021 gttcttctcc atcagcagtt ccgggaaaag tctggaggtc atcgccaccg atggcagcgt
     1081 ggtccgccgg ctccctcttc cagcggtgcg tccaggcgat caactcgttg gactgaatat
     1141 ctccggcaac ctggccttga tgacgtacat gaactttaag gccacacccc ggtccagcta
     1201 tcttctgctc aacgcggcaa ccggcgagcc ctatcggttt tttctgccgc cggcggctgt
     1261 tcaggggagc ctgacatgct tcgactcatc gaggggattc acgtttctct ccggtggcaa
     1321 cggacatctc agcctgattc agtcggcact accctagtcc gaccgtcccc cacccgctac
     1381 tccgccgtct tcaccagata cgagctcccg aacagcgcca tccccaccag cagcggaatc
     1441 atcccgcaca gccatacccc ctgctcgctc cccagcagcg cgcgcagaaa aatcagcacc
     1501 cccagccccg ccgccgccgt caccagcccg ccgatctgca atccctgcct cgttcgcagc
     1561 gtggacaagc gcgcctcttc ccgcatcaac tccagcgccg aattggctcc aggcccctgc
     1621 gcgtcggcca gcttcttcag catgtcgttc ttgtagtagg cctcgcgctc cttctgccgc
     1681 gctcccgacc aggtcgcgac cgagatcatc gaaaacagcg ccaccgcacc caccgccaga
     1741 aacacaaaaa ccatcgatcc cggtcccatc gctccaatgt cgatcatgag aaatcccctc
     1801 ctccgcggac cccgttttct gtccgcttca cccggtacga ccagcgcacc cttcccgcgg
     1861 ttgcgatttc tttgcagaaa tctctcgaca caaaaattga aaccgcgcac catcgtccct
     1921 ggtcctaccc gccaggaaga ctacgagtga acccggaccc cgccagaccg gaatcgagcg
     1981 acgaagccct caacgaccag gccccgaacg acgaagctct cgtcgcccgc gtcgtcgccg
     2041 gtaacaccgc cgccttcgaa ggcatcgtgc gccgctggca atccccgctc gttaacctcg
     2101 cctggcgctt ctgccgcgat cgcggacgcg ccgaagaaat ggctcaggaa gccttcctcc
     2161 gcgccttccg caatctcgcc cagtttcgtg gcgactccgc cttctccacc tggctcttcg
     2221 ctctcgccac caatctgtat cgcaccgaaa ttcgccgcct gccctccatg ccgctctccc
     2281 tcgacgaaat cgccgaaccg cccgaccccc gcatcgccgc tgccacagcc cttgaagaag
     2341 ccgaccgcgg cgagtccatc cgccgcgccg tcctcgccct gcccccgaag tatcgcgacg
     2401 ccgtcctcct cttctatttc cacgagcagg atgtgccggc cgccgcccgc accctccgca
     2461 tcccggaagg caccgtaaag gctcgcctct tccgcggccg cgaactgctg cgcgccaaat
     2521 tttcacgggc ccgcgtttcg aaatcgacgg gcctcgcatc cgattcctcg acggaggtac
     2581 gaacgctatg aaaacgagaa cccatcgtcc cccgctcacc gacgccgacc tcgaccgcgc
     2641 cctcgcgccg gccagcgaca gcattctccc ctcctcggga tttgccgcga gcgtcatggc
     2701 cgccgttcac gaagagcaag cggctcccgc tcccattcct tttccctgga agcgcgccgt
     2761 gccgggcctc ctcgccgtcc tcgccgccct cgcgatcctg atcgtcgcag ttcccgctgc
     2821 tctccggtcg gcgatggccg ccgctcagca acaccaggca gccggctcga tccagggatc
     2881 gctcaccgct ctggcccatc caggcaatcc gacgacctgg atctccgcct ccgtgattct
     2941 cagcctggca tgtctgctct tctgccggaa gctgatcgcc cgctaaaagc gactcacgcg
     3001 ggcccactcg tcgttcgctc ggctcatgct cgactcatgt cggtccatgc tcgcttttcg
     3061 ctgaccggac tttagccgac cgcttcttag ctgaccggcc tttcgccgac cgcaccttag
     3121 ctgccgctat aattcctgcc aaccatgtcc accacgatcg cgggccctgt ctccttcgtc
     3181 ctcaccgacg gcgccacccg ccgcatcctc accatcacgc actccccgtt caccatcggc
     3241 cgcctccccg agaacgacct cgtcctcgcc catcccttcg tctcgcgccg ccatgccgac
     3301 ctcatcctcg aaggcgatcg cgccttcatc gccgatcagg ccagccgcca cggcaccttc
     3361 gtcaacggcc agcccacacc cggtcgccat gccatcggcg ccagcgacac catccacttc
     3421 ggcgcgctcg acggccccag cctccgcctc agtaccgatc acgccagcgc cgatttcccc
     3481 agcggcacct ccaccatccg caacatcatc gagcaaatcc cgggagtcgg cgccggcaac
     3541 cccctcgaca agctccgctg gttctttgaa tctgcacgca agctcaacac ctccgacgcc
     3601 gtcgatcaga tcctcaccgc cctgctcgac accacgctgc agctcaccca ggtcgagcgt
     3661 ggctacgtct tcctccgcaa ggaaaccggc gaaatggaac tcgcccttgg gcgcgacaac
     3721 aaaggcgagg ccctcaccga tcagtcgacg ctttcccacg gcgctatgcg ccaggccgtc
     3781 agcacctcca acgagttcat cgtcaccgat accctcagcg ccgactccga tacccgctcc
     3841 gccagcatgg tcgcccacag catccgctcc gtcatctgca tcccgctgcg caagcgccgc
     3901 cctggagccg ctcgcgagac cgatctgctc ggcctccttt acctcgacag ccgtctcaag
     3961 cccggcatgc tcaccgagat cgacaacgac ctcctgcgca ccatctccac cgacgccgcc
     4021 gctctcattg acaacgccca gctcgccgtc tccgaagaac gcgagcgcgt ctatcgccag
     4081 gaaatgaaca tcgctgccga cattcagcgt ggcctcatga ccgtccgccc gcccacgctc
     4141 acctgggcga aggtggccgc ccgctccgtg ccctgccgcg aatgcggagg cgacttcttc
     4201 gacatcatcg aagacggcga ggctctggcc gtcgtcgtcg ccgatatttc cggcaaagga
     4261 atctccgctg ccatcctcgg cgccacgctg cagggcctca tgtattcgct gctcgtggcg
     4321 cgccagccgc tggccaccat cgccgaaatc gtcaatcgct atatctgcgg caagaaagtc
     4381 gaaaagtacg ccaccatggc cgtcctccgc atttcgccca atggccacat ggagtacatc
     4441 aactgcggcc acgtcccgcc cttcctctat tccggaggct gcacgcgtct ctccgaaggc
     4501 aatcttcctg tcggcctgat tgaaggagcg cccttcacct ccggaactct tcagctccag
     4561 cccggcgctc gcgttctaat tgtcactgac ggtgttacag aagctgccga tctcggcggc
     4621 gagttctacg gagaaccccg cctcaccgcg ccggaattcg agagcctcac cctcgacgaa
     4681 atcttctctg acgtcgaaaa attcgccggc tccctcgccg ccgacgacga ctgcaccgtc
     4741 atggacgtcc gctacctcgg ctgaaccggc gtcggcgata gtcccgccgc cggctcaacg
     4801 tccacgcgaa cccggcttcc cgatcaccat cgaagtttca acgagccccg aactcgtctg
     4861 cgcggagccg gaaagaaatc cgctcctccg cgtcagcact cggtgaacgt gggcagccca
     4921 gctgcgcaaa ccgcgatccg aaaaatggcc tccggccttc gcgccccctg agtccagctc
     4981 ccctgtttcc agccactccc tgaccggcac cacaaatccg ctcttcggac ggttaagaat
     5041 cttcgccggc agaggctttc gcagacactg caccatccct ggcttgcgcg gagaaaaacg
     5101 gctcgcccgc agggcggaga tctgccgcaa caaaaccata tccaccagcg gcacccgtat
     5161 ctcgaccgaa tgcgccatac ccgcccagtc ggcatcccgc aataattgcc tgcgcatgta
     5221 gtacctcgtc tccagcgccg aaacacgcag gaaatcggcc tgctctttgg ccacattcct
     5281 gagattccca aacggctgca ccagggcgtt catctcctcg atggctccca gctttcgcca
     5341 tccttcccgc accaggtccg catcgagaaa acccggaagc tcccatggca taaacaatcc
     5401 ccgccgcagc agataagcgc caccccagct cccgccatat tcaaacaggc tcgcgtactt
     5461 cggtgacgtg aaacgggaaa tcattccctg ggtcacaacg cgcacaccac gccccaccca
     5521 cggcgtccgc cgaagcggcc tcaatccctt cacaagcttc ggaatctgat gaaagctggg
     5581 ataaccgcca aaaagttcat ctccccccag cccgctcaac gcgaccttaa aaccccgctc
     5641 acgcgccagc cagctgatga agtaggtgtt caccccatcc accgtgggct gatccatctg
     5701 ctccagcagt cgatcccggt gttctgcaaa gatcgactga tcgacaaatc gcgtgtagtg
     5761 aggcaagcca tagtggccag ccacctccgc cgcatcgtcc gtctcatcct cagccgtgcc
     5821 gcgcagccga tcaaaaccca gcgtgagcgc ctcgacatcg ttgcccgact cgccggcaag
     5881 cgaagcgatg atcccggaat cgatccccgc cgaaagaaaa atcgccaccg gcacgtctgc
     5941 gatttcgtgc atccgcaggc tctctctcag agcgtcgtgc aggatgacat tcgcttcctc
     6001 ctgcgatgtt ggcatggccc ccgcatcgaa ctgcagagcc tcgtccactg gagacgcaaa
     6061 gcgttttggc ggcgagatcc cgtgcccgct ggcccgcatc caggtcccgc ttggcatgca
     6121 gcggattccc cgatacagcg tgtacggctc aggaaccgag ccccacacaa aaaaacctac
     6181 atggccggcc ggctcgtcct gcaaatccac gtccggcacg ccgatgagcg ctcttacctg
     6241 cgacgcgaaa taaaatcggc ctccgccctc ggcgtaatac agcggcttga taccgaacgg
     6301 gtcgcgcgcc aggaacaact cttcagcctc ctgatcccag atcgcgaacg catacatgcc
     6361 gcgcagccga ttcaacatcg ccgctccgca ttccgcgtac atcgccagca ggacttcggt
     6421 gtcgctcgtg gtcctgaatc gccgcccctt cgccttcaac tcctctcgaa gttcctggta
     6481 gttgtagatt tcgccgttga aaacgatcgt gtagcgtcca tcgccggaat gcatcggctg
     6541 atccgcccgc acgtcgagat cgatgatcga caaccggcgg tgtgcgaacc ccatcctgtc
     6601 atcaggtcca atccactgtc ccgacgcatc cggcccacga ctctccatcg tcgcggacat
     6661 gcccgccaga acaatcggga cgtcaacctg cgttgaattt ttcagacaca agagcccggc
     6721 aataccgcac atcaggacac caaaccttta ggggaggttc gcagtcaaga tttacttgaa
     6781 accgatagcc taaccgttgc cgcttccgtg cgatggggca accctcgcac tgccatcgct
     6841 gaacgatgac ctcgattcat ctgagaaggc gagcgcggga agggcggatg gaccagggcg
     6901 ggacagcatg cgtacgccat aaacgccaaa ggagtggtgc tgaaatgggc tcccgcggat
     6961 cgatagagct ggactcgaat cctctcggat agactgacac aggtcccttc ccgacgaaat
     7021 gaaaatcctt catgtcatcg gaagcctctc tccagccgat ggtggccccc ccgaggctgt
     7081 gcgtcaactt gcacgcgcct atcttcagat aggagttgaa gttgaggtgc tctgccagga
     7141 tgctccagcc gcctcgttcc tcgccgagct ttccgttccc gttcatgccc tcggccagcg
     7201 ctggctcggc cgcttcggcc tttcgccgcg cctctggcgg tggctgcatc ggaacgcctc
     7261 ccgtttcgat ggcatcgtga tgaacggcct ctggatcttt cccgatcttg ccgtccgctc
     7321 cgccgctcgg cgtgctggaa tcccctacgc cgtcttcgcc cacggcgctc tcgatccgtg
     7381 gttcaacacc cgctacccgc tcaagcacct caagaagcgc ctctattggc cgctccagta
     7441 tcgtgtcctg cgcgacgccc gtgccgtcct cttcaccacg cccaccgagc gcgatctggc
     7501 cctgaccagc tttcaaccga atcaatggaa cagcctgccc gtcccctatg gcatcggcga
     7561 gccaaccggc gatccctccg ctcaggtcga gctcttctac cgccaggttc ccgaactgcg
     7621 cggccgccgc ttcctgctct tcatgtcccg gctccacgct aagaaaggct gcgacctcct
     7681 cgttgaggcc ttcgccaggg ttgcggccgg ccaccccgac gtcgacctcg tcattgccgg
     7741 tccggatcag cagggtttcc aggcgcagct ccagcgtacg tgcgcagaaa agggcatcgg
     7801 cgaccgcgtc ttctggccgg gaatgatccg cggcgacctg aaatggggcg cgcttcatgc
     7861 cgccgaagcc ttcatcctgc cctcccatca ggagaacttc ggcatcgtcg tcgtcgaggc
     7921 cctggctgcc ggcacacccg tcctcaccac caacaaggtc aatatctgga aagagataca
     7981 ggaagatgga gctgccctga tcgaggacga tacccttgag ggcaccgggc gcctgctgca
     8041 ccgctggctg cggttttccc ccgccgagcg ccagaccatg gctgcccagg cccagccctg
     8101 ctttcgcgcc cgctactcga tgagagaaac cgcgatcgtc atccgcgacc tcttcgctca
     8161 ggcgaggcca aaaagtagag cgtctatctg acgaaaaaca agtagattat cgccggtaac
     8221 gtgacttccg gccaacccat cccgcaacct ttccaccagt accgttacct ctgcagccgc
     8281 tccagcagcc ggtcgaactc ctccagctgc ccgtattcga tgattacccg cccccgcccc
     8341 ttcttgtcct cgatccgcac cttcagcccc aaagccctct gcagtgactc ctgcacttcg
     8401 cggacattcg ggtcgacggg tttctccttt tgttcgccat ccgcggcagg cgccggagtc
     8461 agtagcttct gaacataact ctctgtctgc cgtacggaca tggagagcgc caccaccttc
     8521 tgcgccgctt tctgaatcgc ttgcggatcc tcgagcggca gcagcgtccg cgcatgcccg
     8581 aaactcagaa ccccttcttc gaccttggtt tgcacctcag tcgggagctt caacaggcgc
     8641 agaaaatttc ccacggaagc ccggtccttg ccggtgcgct tcgccatctg ctcctgcgtc
     8701 agctggaact cccgcgccag ccgctcatac gcccgtgcct gctccatcgg gttcaggtcg
     8761 ctccgctgca ggttctcgac aatggtcatc tccatcgcct gctcgtcgga tacctgccgg
     8821 acgatcgccg gaatcgtctc cttgcccgcc cgctgcgaag ccagccaccg ccgttcgccg
     8881 gcgatcagct ggaagcgtcc atgaggcagc ggccgaacca caatcggctg caccaccccg
     8941 gtcgccgtga tcgacgccgc cagctccgcc agctgcacct cgtcaaacct cgaccgcgtc
     9001 tgaaagggac tgcggtcgat ctcgcccacc gga
//
LOCUS       DASZYN010000066         6572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1095455, whole genome shotgun sequence.
ACCESSION   DASZYN010000066 DASZYN010000000
VERSION     DASZYN010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6572
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095455"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VHE33_01805"
     CDS             <1..956
                     /locus_tag="VHE33_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HVT96210.1"
                     /translation="PDPDPVYTRFFEPAMGSLPADLDVIGKAYIAALAEEIAQWLRSI
                     PEQEPIGVCFSGGIDSGSVFLTTYHVMRKLGMNPSRLKAFVLDLGDGPDVQQARDFLE
                     KLDLGLFLEPVDGDPGMLDVGETIRIIEDYKLLDVESATMALALARGIRARYPEWRYL
                     IDGDGGDENLKDYPIEENPELTIRSVIHNSMLYQEGWGVGRIKHSLTYSGGLSRSYVR
                     TYATARHCGFEGFSPYTRPEVIAVAEAIPFIELTQYSVERLYELKGEIVARGVRAVTG
                     LEMPVFPKRRFQHGAVSKAALRERMPFREAEYREQFLALYR"
     gene            940..1923
                     /locus_tag="VHE33_01810"
     CDS             940..1923
                     /locus_tag="VHE33_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96211.1"
                     /translation="MHFTVEVLVTAYPAALTERDAWIVARRGARHAVDPMRPWAFFVE
                     EERSASGEIVSIATIFLTNRECPWRCLMCDLWRNTLTGETPVGAIPAQIDFALERLPA
                     SQQMKLYNSGSFFDRKAIPVEDHAGIAERVRGFERVIVECHPALVGDDCLRFRDRLSG
                     KLEVAMGLETVHEQVLGRLNKRMTLESFRGAADRLRENQIDLRVFILVRPPFMREEEA
                     VEWAARSVDFAFECGATAATLIPTRGGNGAMEELATGGDFAPPSLSSVEAAAEYGVHR
                     GRGRVFVDLWDLARVAGCGACSGQRVDRLRRMNLRQEALPRVICEECGGQS"
     gene            1905..3497
                     /locus_tag="VHE33_01815"
     CDS             1905..3497
                     /locus_tag="VHE33_01815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVT96212.1"
                     /translation="MWGAELNDVYDIAVVGSGFAGSLLAMIACRLGRSVILIEKGTHP
                     RVVIGESSTPLSNLLLEELATRYDLPALLPLAKWGTWQRAYPNIAVGLKRGFTFHHHE
                     LGAAHIDDPDRGDQLLVAASPHDAIADTHWYRADFDQLLVEAAKKLGAEYVDRVNLRQ
                     FEEDERGVWLRGERQGVEVQFRARFVIDATGPRGFLHRALQIRERELPDFARTQALYS
                     HFSGVGRLENGRFDRSAGAAPPYPIDDAAVHHIFDGGWIWVLQFNNGITSAGVAATDV
                     CASRLRMDEGEAAWKRLLEGIPALQEQFSGATAVRPFTHMPQVGFRSERMVGNRWALL
                     PSAAGFVDPLLSTGFPLTLLGVERLARILEDEWEKPGFAASLQEYAEQSDRELLATAR
                     LIGALYANMSNFPVFIALTLLYFAAASFSETARRLGKPDLATSFLLCEDPVFGPASRN
                     LIERARRTMTCGESEQLIRDIHTVLEPFNIAGLGRAERRNWYPVVAQDLFDGASKLGA
                     THAEVEAMLQRCGFAECKPAAD"
     gene            complement(3494..4354)
                     /locus_tag="VHE33_01820"
     CDS             complement(3494..4354)
                     /locus_tag="VHE33_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96213.1"
                     /translation="MNWRMVAPSVQPRPALWRRILDSRFMPPIFITLILLVGNVSFGI
                     LESYTRTLLAIAISITGELILGRIFLKKWPHPASAYITGISVGILLRSPAYWPYGLCA
                     ALSITSKYALRFRNRHIWNPSNFGICAMLFLASDSVAGLSIQWGNNFVSLVVIWILGA
                     VIVWRARRFNITATYVASFIALSFLRSWITGDPWQSEISPITGPMYQLFIFFMITDPK
                     TTVRSRTGQCVVAIAIALVEFFMRLGSSVYAPLYALFWVGPAALFIEMWLDARRARAV
                     PAVLTGPQPA"
     gene            complement(4351..6141)
                     /locus_tag="VHE33_01825"
     CDS             complement(4351..6141)
                     /locus_tag="VHE33_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HVT96214.1"
                     /translation="MKRLPIARIVVTIVFIGMLATPWVIRHFSHAQARPATSTVDCQA
                     ALSRYGFCFQEVAHQAGIRFVHEAPILDHKLDGIMPEVASMGAAVSIVDFDRDGWPDI
                     YVTNSRTGSQNHLYRNNHDGSFTDVAQQMGIADVNQSGTGVSMGAVWGDYDNDGYDDL
                     FLIKWGRPELFHNDQGHGFTRVSDQAGLPPWINANTALWFDYDGDGRLDLFVAGYYPE
                     DVDLWHLKNTRMMPESFEYAKNGGRKYLFHNLGDGHFEEVSAKVGISSRRWALASAAV
                     DLRGTGYPDLFVANDYGVSELYLNDHGHFVESGEQTGVGFAPKSGMNAAFGDIFNQGK
                     YSIYVSNISEEGILLQGNNLWVPQDGPGLHYANLARDLGVELGGWSFGAQFGDLNNDG
                     NLDLFLENGYISLARSRSYWYDFSKVAGGNSSIISDAAHWPAFNGRSLSGYQQKKVWM
                     NDGAGKFEDVAQAVGVTDTYDGRAIALADLWNTGVLDAVVANQSGPLLIYKDTAAPGN
                     NWIEFALEGTKSNRSAIGAQVTLFWSGQKQMQYVSGGNGFASENDRRLHFGLGANAQI
                     DKAIIRWPSGTEQTLDHLVPNQLVHVKESQ"
     gene            complement(6138..>6572)
                     /locus_tag="VHE33_01830"
     CDS             complement(6138..>6572)
                     /locus_tag="VHE33_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96215.1"
                     /translation="DYNHIASTTQIWRDLQGFYTRFGDVRPLLNKIDDRYIIMNAGDE
                     LALRFSAPAPPPAGWVRDYVLAGDGWIKDGDYNSTFSATVQPLPYHGKSWYDAPPGPL
                     EAEWTYRHHPEDWLIWQTRYVTPQPFRDALNDRPQQAETAPR"
BASE COUNT         1231 a         1666 c         2355 g         1320 t
ORIGIN      
        1 gtcctgatcc ggacccggtt tatacacggt tttttgagcc ggcgatgggg tctctgcccg
       61 cggacctgga tgtgattggc aaggcgtaca tcgcggcgct ggcggaggag attgcacagt
      121 ggctgcgatc gattccggag caggagccga ttggggtttg cttctcgggt gggatcgata
      181 gcggcagcgt ttttctaacg acataccacg tgatgcggaa gctcgggatg aatccgagcc
      241 gattgaaggc gtttgtactc gacctgggcg atggtccgga tgttcagcag gcgcgggatt
      301 ttctggagaa gctggatttg gggttgtttc tggagccggt tgacggcgac cccggcatgc
      361 tcgacgtggg cgagacgatt cgtatcattg aagattacaa attgctggat gtggagtcgg
      421 cgacgatggc gctggcgctg gcgcgaggga ttcgcgcacg gtatccggaa tggcggtatc
      481 tgatcgatgg cgatggcggc gacgagaatc tgaaggatta tccgatcgag gagaatcccg
      541 agctgacgat acgcagtgtg attcataact cgatgctgta ccaggaggga tggggcgtcg
      601 gcaggatcaa gcactcgctg acgtacagcg gaggactgag ccggtcgtac gtgcgcacgt
      661 atgcgacggc gcggcattgc ggctttgagg gattcagccc gtacacgcgg ccggaagtga
      721 ttgccgttgc ggaggcgatt cccttcatcg agctgacgca gtacagcgtg gagcgactat
      781 atgagctgaa gggcgagatt gtagcgcgag gcgtgcgagc ggtgaccggc ctcgagatgc
      841 ccgtatttcc gaagcgtcgc tttcagcacg gagcggtctc gaaagcagct ctgcgagagc
      901 ggatgccgtt tcgcgaggcg gagtaccgcg agcagttcct tgcactttac cgttgaggtt
      961 ttggtgacgg cgtatccagc ggcgttgaca gagcgggatg cgtggatcgt tgcgcgacgc
     1021 ggagctcgcc atgcggtcga tccgatgcga ccgtgggcgt tctttgttga agaagagcgg
     1081 agtgcttcgg gcgaaatagt ttccatcgcg acgatcttcc tgacgaatcg cgaatgcccg
     1141 tggcgctgcc tgatgtgcga tctgtggcgg aacacattga caggcgaaac gccggtgggg
     1201 gcgattccgg cgcagattga ctttgcgctg gaacggttgc cggcatccca gcagatgaag
     1261 ctctacaaca gcggcagctt tttcgatcgc aaagcgattc cggttgagga tcatgccggt
     1321 attgccgaac gggtgcgtgg gtttgaacgc gtgatcgtgg agtgccatcc ggcgctggtc
     1381 ggcgatgact gccttcgctt cagggatcgg ttgagcggga agctggaagt ggcgatggga
     1441 ctggagacgg tacatgagca ggttttgggc cgactgaaca agcggatgac gctggagtcg
     1501 tttcggggcg ccgcggatcg cctgcgcgag aatcagattg atttgcgggt gttcattctg
     1561 gtccggccgc cgttcatgcg cgaagaggaa gctgtcgaat gggcggcacg atcggtggat
     1621 ttcgcgtttg agtgcggggc gacagcagca acgctgattc ccacgcgcgg ggggaatggt
     1681 gcaatggagg aactggcgac gggtggagat tttgctccgc catcgctttc gtcggtggag
     1741 gctgcggcgg agtatggagt gcaccggggc cgtgggcggg tgtttgtgga cctttgggat
     1801 ttggcgcgag tcgcaggatg tggggcgtgc agtgggcagc gcgtcgatcg attgcggagg
     1861 atgaatctgc gacaggaggc tctgccgcgc gtgatctgtg aggagtgtgg ggggcagagc
     1921 tgaacgacgt ttacgacatt gcggttgtcg gatccggatt tgccggatcg ctgctggcga
     1981 tgatcgcgtg ccggctcggg cgctcagtga ttttgatcga gaagggcacg catccgcgcg
     2041 tggtgattgg cgagtcgtcg acgccgctgt cgaatctgct gctcgaagaa ctggcaactc
     2101 gctatgatct gccggcgctg ctgccgctgg cgaaatgggg cacgtggcaa cgagcgtatc
     2161 cgaatatcgc ggtggggctg aagcgtgggt tcacgttcca tcatcatgag ctgggcgcgg
     2221 cgcacatcga cgacccggat cgtggcgatc agctgctggt ggcggcgagt ccgcacgatg
     2281 cgattgctga cacgcactgg tatcgcgcgg acttcgacca gctactggtg gaggcagcga
     2341 agaaactggg cgcagagtat gtcgatcggg tgaacctgcg tcagttcgaa gaagacgagc
     2401 gtggggtttg gctgcgagga gagcggcaag gggtcgaggt ccagttccgg gcgcggttcg
     2461 tgatcgatgc cacggggcct cggggatttc tgcatcgcgc gctgcagatt cgcgagaggg
     2521 aactaccgga ctttgcgcgc acgcaggcgc tgtattcgca tttttcggga gtggggcgtc
     2581 ttgagaatgg ccggttcgat cgaagcgccg gcgctgctcc accgtatccg atcgatgatg
     2641 ctgcggttca tcatattttt gacggcggtt ggatctgggt gctgcagttc aacaacggaa
     2701 tcaccagcgc cggtgtcgca gcgacggatg tgtgcgcgtc acggttacga atggatgagg
     2761 gggaagctgc gtggaagcgt cttctggaag gaattccggc gctccaggag caattttcag
     2821 gggctactgc agtgcggccg ttcacgcaca tgccgcaggt gggctttcgc tcggagcgga
     2881 tggtgggcaa tcgctgggct ctgttgcctt ctgcggccgg ctttgtcgat ccgctgctct
     2941 cgaccggttt tccgctgacg ctgctcgggg tggagcggct cgcgaggatt ctggaagatg
     3001 aatgggagaa gccgggcttc gcggcgagcc tgcaggaata tgccgagcaa agtgacagag
     3061 agctgcttgc gacggctcgg ttgattggcg cgctgtacgc gaacatgagc aacttcccgg
     3121 tttttatcgc actcacgctg ctgtattttg ctgcagcgag tttttcggag acagcgcgaa
     3181 gactaggcaa gccggatctg gcaacgtcat ttcttctctg tgaggatcct gtgtttggac
     3241 ccgcgagcag aaatttgatc gagcgtgcgc gtcgcacaat gacttgcggc gagtcggagc
     3301 aactgatccg cgacattcat actgtgcttg agcccttcaa cattgcggga ctggggcgag
     3361 cggagcgccg aaactggtat ccggtggtgg cgcaggatct gttcgatggt gcgagcaaac
     3421 tcggcgcgac gcatgccgag gttgaggcga tgttgcagcg ttgcgggttt gcggagtgta
     3481 agccggctgc ggattaagcc ggctgcggtc ccgtgagcac tgcgggaaca gcacgagcgc
     3541 gacgcgcgtc cagccacatc tcgatgaaca aggccgcggg tcctacccag aagagggcgt
     3601 acaacggtgc gtagacactg ctaccgagac gcatgaagaa ctccaccagg gcgatggcga
     3661 tggcaacgac gcattggcct gtgcgcgagc gcacggtggt cttcgggtcc gtgatcatga
     3721 agaagatgaa gagctgatac atggggccgg tgatgggtga gatctccgac tgccacggat
     3781 cgccggtgat ccacgagcgc aggaatgaga gcgcgatgaa ggaggcgacg tacgtcgcgg
     3841 tgatgttgaa gcggcgcgcg cgccagacga tcaccgcgcc aagaatccag atgacgacga
     3901 gcgacacaaa gttgtttccc cactgaatgc tgagacccgc aacggaatcg gatgcgagaa
     3961 acagcatcgc gcagatgccg aaattggagg ggttccagat gtggcgattg cggaagcgga
     4021 gagcgtattt cgaggtgatg gagagggctg cgcacagacc atagggccag tacgcggggg
     4081 atcgcagcag gatgccgacg ctgatgccgg tgatataggc gctggccggg tgcggccact
     4141 ttttcagaaa gatgcggccg aggatgagct ccccggtgat gctgatcgcg atggcgagga
     4201 gcgtgcgtgt gtagctctcg aggattccga aggagacgtt gccgaccagc aaaatgagcg
     4261 tgatgaagat gggcggcatg aagcggctgt cgagaatgcg ccgccagagc gcgggccggg
     4321 gctggactga tggagcgacc atcctccagt tcattgagac tccttcacgt gaacgagttg
     4381 attcggcaca aggtggtcga gggtctgctc ggtccctgaa ggccagcgga tgatggcttt
     4441 gtcgatttgc gcattcgcgc caaggccgaa gtgcaggcgc cggtcattct ccgacgcgaa
     4501 gccgttgccg ccggagacgt actgcatctg cttctggccg ctccagaaca gcgtaacctg
     4561 ggcgccaatg gcgctgcgat tgctctttgt gccttcaagg gcaaactcga tccagttgtt
     4621 gccgggagct gcggtgtctt tgtagatcaa cagcgggccg gattgattgg ccacgaccgc
     4681 gtcgagcaca ccggtgttcc agagatcggc gagcgcgatg gcgcggccgt cgtaggtgtc
     4741 ggtgacaccg acggcctggg cgacatcttc gaacttgccg gcgccgtcgt tcatccagac
     4801 ctttttctgt tgatagccgg agaggctgcg gccgttgaag gcgggccaat gggcggcgtc
     4861 gctgatgatg gagctgttgc cgccggcgac tttggagaag tcgtaccagt agctgcgcga
     4921 gcgcgcgagg gagatgtagc cgttttcgag gaacaggtcg aggttgccgt cgttgttcag
     4981 gtcgccaaac tgcgcgccga agctccagcc accgagttcg acgccgagat cgcgggcgag
     5041 attggcgtaa tgaaggcccg ggccatcctg cggaacccag aggttattgc cctggagcag
     5101 gatgccttcc tcggagatgt tcgaaacgta gatggagtat ttgccctggt tgaagatgtc
     5161 gccaaaggca gcattcatgc cgcttttggg ggcgaagcca acgccggttt gttcgccgga
     5221 ctcgacgaag tggccgtggt cgttgaggta caactccgaa acgccatagt cgttggcaac
     5281 gaagaggtca gggtagccgg tgccgcgcaa atccacggcc gcggaggcga gcgcccagcg
     5341 gcgactgctg atgccgacct tcgcgctgac ttcctcaaag tgaccgtcgc cgaggttgtg
     5401 aaagagatac ttgcggccac cgttctttgc gtattcgaag ctctccggca tcatgcgggt
     5461 gttcttcagg tgccagagat cgacgtcctc gggatagtag ccggcgacga acagatcgag
     5521 ccggccgtcg ccatcgtagt cgaaccagag cgcggtgttg gcattgatcc agggaggaag
     5581 accggcctgg tcgctgacgc gcgtgaatcc gtgaccctga tcgttgtgga acagctccgg
     5641 gcggccccac ttgatgagga aaagatcgtc atagccatcg ttgtcgtagt caccccacac
     5701 cgcgcccatc gaaacgccgg tgccggactg attgacgtcg gcaatgccca tctgctgcgc
     5761 cacgtcggtg aaggagccgt cgtggttatt gcggtagaga tgattctggc tgccggtgcg
     5821 gctgttcgtg acgtaaatgt cgggccaacc atcgcgatcg aagtcgacga tggaaactgc
     5881 ggcgcccatg gaagcgactt cgggcatgat gccgtcgagt ttgtgatcga ggatgggggc
     5941 ttcgtgcacg aaacggatgc cggcctgatg ggcgacttcc tggaagcaga agccatagcg
     6001 agaaagagcg gcctggcagt cgacggtgga ggttgcgggc cgtgcctggg catgggaaaa
     6061 gtggcggatc acccagggcg tggccagcat gccgatgaac acgatggtga cgacgatgcg
     6121 ggcgatgggg aggcgcttca tcgtggcgcg gtctccgctt gctgggggcg atcgttgagg
     6181 gcgtcgcgaa agggttgcgg cgtgacgtag cgcgtttgcc agatgagcca gtcttccgga
     6241 tggtggcgat acgtccactc cgcttcgagc ggtcctggcg gcgcgtcgta ccaggatttg
     6301 ccgtgatacg gcagcggctg cacggtggcg gagaaggtgg agttgtagtc gccgtccttg
     6361 atccagccat caccggcaag cacgtagtcg cgtacccatc cggcaggcgg cggcgctgga
     6421 gcagagaagc gcagggccaa ttcgtcgccc gcgttcatga tgatgtaacg gtcatcgatc
     6481 ttgttcagca gcgggcgcac atcgccgaag cgggtgtaga aaccctgcag gtcgcgccag
     6541 atctgggtag tggatgcgat gtggttgtag tc
//
LOCUS       DASZYN010000067         2424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_67549, whole genome shotgun sequence.
ACCESSION   DASZYN010000067 DASZYN010000000
VERSION     DASZYN010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67549"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VHE33_01835"
     CDS             complement(<1..219)
                     /locus_tag="VHE33_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96216.1"
                     /translation="MRLTPLIPFLLCIALHAQDVHVRVSPDVKTGIEGTAEFPTIQMA
                     LDHHPFAGPGGRVFIQIAPGTYHERLNIT"
     gene            complement(354..2027)
                     /locus_tag="VHE33_01840"
     CDS             complement(354..2027)
                     /locus_tag="VHE33_01840"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HVT96217.1"
                     /translation="MRYPTTLAASLFAATLASTLTISAPAQTSWTSTATRGISVTQLE
                     SAADYGPLPTDQAMTVRVALRLQNQAALNTYIRNINDPANPLYGQSLTPEQFAVSYAP
                     SAAQVQQVVSYLQGAGFTNVAVEPNNLIVSADGTAAQASAAFNTPIEQFAQFGKLVYG
                     NTAPAQVPASLGNIVGAVLGLNTIGQMKPMLLKTSAGGAPQYLVSYTPQNFWQIYSAS
                     DVPAASKATIAVIAEGDLSGVLTDFNAAQASWGFPRIPVTVVPVGLASTDTAGVDEWD
                     MDSQYSTGMAGGLVKHLYFYDATSMTDSDLALAFSRWVTDDKARAANASFGECEIFPY
                     LDGSMLLDDELFAEGAAQGQTLFSSTGDTGSFCPVGVGTNGVPAGVPLVNYPAASQYV
                     TAVGGTTVLTNADGNYNTEVAWNAGGGGISQFEYSPFWQVAANVPSAALSSKGIPDVS
                     MDADPESGANVYVNGAWEGVGGTSLSSPLAMGVWARMISANPKLGFAPITFYSLYDGT
                     GLLGSYPKGGFHDILVGANGLYTAGPGWDFTTGLGTLWVDQLYNDLKRR"
BASE COUNT          499 a          654 c          821 g          450 t
ORIGIN      
        1 cgtaatgttg aggcgctcgt ggtacgtgcc gggagcaatc tggatgaaaa cgcgaccgcc
       61 ggggccggcg aacgggtgat ggtcgagcgc catctggatc gtgggaaatt cggcggtgcc
      121 ttcgatgccg gtcttcacat cgggtgacac gcgcacgtgg acgtcctgcg cgtgcaaggc
      181 gatgcaaagc aggaagggaa tcagaggagt gagacgcatc gtatcccagt gtgccgcggt
      241 gtcccgggaa aagctactcg ggcggaggag ttcgccgcag acgaaaaaga ggggaccgcc
      301 gtttgagcga tcccctcgca agtcagcgcc aggttcggcg cagcagaaag aagttaccgc
      361 ctcttcaggt cgttgtagag ctggtcgacc cacaatgtgc caaggccggt ggtgaaatcc
      421 cagccgggtc cagccgtgta caggccattg gcgccgacga ggatgtcgtg gaagccgccc
      481 ttcggatagc tgccgagcag gccggttccg tcgtacaggc tgtagaaggt aatgggcgcg
      541 aatccgagtt tgggattcgc ggagatcatg cgtgcccaca cacccatagc aagaggtgaa
      601 gagaggctgg tgccgccaac gccttcccac gcgccgttga catagacgtt cgcaccagat
      661 tcaggatcgg cgtccatgga aacatcggga atgcctttgg aactgagcgc ggcgctggga
      721 acattggccg ccacctgcca gaagggcgag tactcgaact ggctgatacc accgccgccg
      781 gcgttccatg cgacttcggt gttgtagttg ccgtcagcgt tcgtcagcac ggtcgttccg
      841 ccgactgcgg taacgtattg cgaggctgca ggatagttga ccagcggcac gccggcggga
      901 acgccgttgg tgccaacgcc aaccgggcag aatgaaccgg tgtcgcccgt cgaggaaaac
      961 agcgtctgcc cctgcgccgc gccttcggcg aacagctcat cgtccaatag catggagccg
     1021 tcgaggtagg gaaagatttc gcactcgccg aaggaggcat tcgcagcgcg cgctttatcg
     1081 tcggtgaccc agcggctgaa cgcgagcgcc aggtcggagt ccgtcatcga agtggcgtcg
     1141 tagaagtaaa ggtgctttac aaggccgccg gccatcccgg tggaatactg cgaatccatg
     1201 tcccactcgt cgacgccggc cgtgtccgtg ctggcaaggc cgacgggcac aaccgtgacg
     1261 ggaatgcgcg ggaatcccca gctagcctgc gcggcgttga aatccgtgag cacgccgctg
     1321 aggtcgcctt ctgcgatgac agcgatggtt gccttcgagg ccgccggaac atcgcttgcc
     1381 gagtaaattt gccagaagtt ctgcggcgtg tagctcacca gatactgcgg cgcgccgcct
     1441 gcggatgttt taaggagcat cggcttcatt tgcccgatgg tgttgaggcc gagcacggcg
     1501 ccgacgatgt tgccgagcga agcgggcacc tgcgccggcg ctgtgttgcc gtagacgagc
     1561 ttgccgaact gcgcgaactg ttcgatcggt gtgttgaacg cggcggaagc ctgagcagcg
     1621 gtgccatccg cgctgacgat cagattattc ggctcaacgg cgacattggt gaagcccgcg
     1681 ccctgaagat agctcacgac ctgctgcacc tgtgcggcgc tgggggcata gctgacggca
     1741 aattgctcag gtgtcaggct ctgtccgtag agcggattcg caggatcatt gatgttcctg
     1801 atgtacgtat tgagcgctgc ctgattctgc agcctgaggg cgacgcgaac ggtcatggcc
     1861 tgatcggtgg ggagaggccc gtagtcggcg gccgactcga gttgtgttac cgagatgcca
     1921 cgagtggcgg tggaggtcca gctggtttgt gcgggggcgg agatagtgag ggtggaggcg
     1981 agcgtggcgg caaaaagcga ggccgcaaga gtggtggggt atcgcatgcg tgtggtctcc
     2041 tcagggtttt cgcactgcgt ggggccgaac tgcgtcggtc aaaattacgt gaaccgaacg
     2101 tatccaaaat gcgcgcagag aaaggttggt tgtcctgtgc gaaggacgga acgcgcgaaa
     2161 tttgggacaa gttgagcttc ttgaaataag tgggaaaatt cgtcacaacg acggtggcag
     2221 aaagttcctc aattcgggaa aaagtatctg cactttaaat tcgtggagaa aacaatagcg
     2281 gcccgttttt cccacgaaat gccatcgttc gatagcaacg agaaaacaga tccggagctg
     2341 acgctcagct ccacagccga acgagcggcc cggtcggccc ggtgacggga tcggacttcg
     2401 ggaagggaag atgcttcatg cgct
//
LOCUS       DASZYN010000068         2352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_710225, whole genome shotgun sequence.
ACCESSION   DASZYN010000068 DASZYN010000000
VERSION     DASZYN010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710225"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1953
                     /locus_tag="VHE33_01845"
     CDS             <1..1953
                     /locus_tag="VHE33_01845"
                     /inference="COORDINATES: protein motif:HMM:NF027792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5054 domain-containing protein"
                     /protein_id="HVT96218.1"
                     /translation="KCHFDAGFIDTQTAVVHKYFSQYFPQAIRVAQQSREPGQHRYVW
                     TTGSWLLYEYLEQASTQDRRIMEDAIAAGDIAWHAIPFNWQTEMMDASMISASIGLSH
                     ALDSRFGRRTTGAKMTDVPGHTRGIVAPIAAEGVKFLDIGVNDASTPADVPPLFLWKD
                     STGASLVVMYHSGYGAVTVVPGSDLAIGIVVRGDNSGPHTPEEIAKVYADLGARFPGA
                     DIVATNLSEIANAVEPFRSHLPVVTQEIGDTWIYGVASDPVKVARYRELARLRRDWIR
                     RGAIRSGDATDLRMLRQVLLEAEHTWGTDTKTWLDFDHYVPKDLATMLDTKNYKVVEF
                     SWQEKRQDLFDGIDTLPEALRREARTSIDQLTPKEPQLPGGKPHPAEQTLETEHFLVA
                     LDPATGAIRQLRNKSTHVDWADSAHPLALLTYQTLSPADYARFFANYIISTADWAKKD
                     FGKPNIELFGAESQEWHPAITRLEVTEDASGHRLLAHLAIKDPQSIASGRAAFPRAMF
                     LELTFPRNEPAVQIALSWFGKPATRMPEALWFTFNPILANANGWTIDKSGESISPLDV
                     VAAGSRHLHAVSTGFANKSGGRSFAVETLDAPLIAVGERSPLNFSREQPDLSTGIHCS
                     LFNNAWGTNYIMWFGEDMRFRFVLRA"
     gene            complement(2101..>2352)
                     /locus_tag="VHE33_01850"
     CDS             complement(2101..>2352)
                     /locus_tag="VHE33_01850"
                     /inference="COORDINATES: protein motif:HMM:NF017071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HVT96219.1"
                     /translation="ATGHPSFVMSNSFSNQTLAQIDLWKNKDTYAIDVYTLPKKLDEE
                     VARLHLEKIGVKLTKLTKEQAEYIGVPVEGPYKADHYRY"
BASE COUNT          458 a          784 c          637 g          473 t
ORIGIN      
        1 aagtgccact ttgacgccgg attcatcgac acccagaccg ccgtcgtcca caaatacttt
       61 tcgcaatatt ttccgcaggc catccgcgtc gcgcagcagt ctcgcgaacc cggccagcat
      121 cgctacgtgt ggaccaccgg ctcgtggctc ctctacgaat accttgagca ggcatcgacg
      181 caggatcgcc gcatcatgga ggatgccatc gccgcgggcg acatcgcctg gcacgccatc
      241 cccttcaact ggcagaccga aatgatggat gcgtccatga tttctgccag catcgggctc
      301 tcgcatgcgc tcgactcgcg cttcggccgc agaaccaccg gcgccaagat gaccgatgtt
      361 cccggacaca cgcgcggcat cgtggctccc atcgccgccg aaggcgtcaa atttctcgac
      421 atcggcgtga acgacgccag cacgcccgcg gacgtgccgc ctctttttct gtggaaagat
      481 tccaccggag cctcgctggt ggtcatgtat cactctggct acggcgccgt taccgttgtc
      541 ccaggctccg atctcgccat cgggattgtg gtgcgcggcg acaacagcgg cccgcatacg
      601 ccggaagaaa tcgcgaaggt ctatgccgat ctgggtgccc gctttcctgg ggccgacatc
      661 gttgccacga acctcagcga aattgccaat gccgtcgagc cgtttcgaag ccatcttccc
      721 gtcgtcaccc aggaaatcgg agatacgtgg atctacggcg tcgccagcga tccggtgaaa
      781 gttgcgcgct accgtgaatt ggcgcggctt cgccgcgatt ggatccggcg cggcgcgatt
      841 cgatccggcg atgcgaccga cctgcgcatg ctccgtcagg ttctgctcga agcggaacat
      901 acgtggggca ccgacaccaa aacctggctc gacttcgatc actatgtccc taaagatctc
      961 gccaccatgc tcgacacgaa gaactacaaa gttgtcgagt ttagctggca ggagaagcgc
     1021 caggacctgt tcgacggcat cgacacgctt ccggaagcgc tgcgcaggga agcgcgcacc
     1081 agcatcgatc aactcacgcc caaagagcca cagctccctg gcgggaagcc ccacccggca
     1141 gagcagactc tcgagacgga acattttctg gtcgcgctcg atcccgccac aggagcgata
     1201 cggcagcttc gcaacaaatc cacccatgtg gactgggcgg attctgcgca cccgctcgcc
     1261 ttgctcacgt accaaacgct ctcgcccgcc gactacgcgc gtttcttcgc caactacatc
     1321 atcagcaccg ccgactgggc caaaaaagat ttcggcaaac cgaacatcga gttgttcggc
     1381 gccgaaagcc aggagtggca tccggcgatc acacgcctcg aggtcactga agatgcaagc
     1441 ggccatcgcc tgctggcgca cctggccatc aaggacccgc aatccatcgc atcgggtcgc
     1501 gctgcctttc cgcgagctat gtttctggag cttacctttc cccgcaacga gcccgccgtc
     1561 cagatagccc tctcgtggtt tggcaaaccc gcgacgcgga tgccagaagc cctgtggttc
     1621 acgttcaacc cgatcctggc caacgccaac ggctggacga tcgacaaatc cggcgagtca
     1681 atctcacccc tcgacgtggt tgccgccggc agtcgccatc tgcatgctgt ctccaccggc
     1741 ttcgcaaaca agagcggcgg ccgctccttc gccgtcgaaa ccctcgatgc ccctctgatc
     1801 gcggtcggcg aacgatcacc cctgaacttc tcgcgcgaac agcccgatct ctccacggga
     1861 atccactgca gcctgttcaa caatgcgtgg ggaacgaact acatcatgtg gttcggcgaa
     1921 gatatgcgct tccgtttcgt gctgcgcgca taatccaatc gcccaaatca aaagccggga
     1981 aggccaatga ccttcccggc tgtcgttttc tgtgacctga gccctgtgat ctgagccctt
     2041 tgatttgagc cctttgattt gagccctttg atttgagccc tgtgatctga gccctttgat
     2101 ttagtaccgg tagtgatccg ctttgtacgg gccttccacc gggaccccga tgtactcggc
     2161 ctgttccttc gtgagcttcg tcagcttcac gccgatcttt tcgaggtgca accgcgccac
     2221 ttcctcgtcc agcttcttgg gcagggtgta gacgtcgatc gcatacgtgt ccttgttctt
     2281 ccacaggtcg atctgagcca gcgtctggtt ggaaaagctg ttgctcatca cgaagctggg
     2341 gtggccggtc gc
//
LOCUS       DASZYN010000069         1869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_515587, whole genome shotgun sequence.
ACCESSION   DASZYN010000069 DASZYN010000000
VERSION     DASZYN010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515587"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VHE33_01855"
     CDS             complement(<1..230)
                     /locus_tag="VHE33_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96220.1"
                     /translation="MKFHLLVMKREGLTGVPFGFERAFFRISQKIQSFAAFCQKNKEC
                     RTHAEFHSVCPVDSAGSSVAGFGSKSGLSHGGG"
     gene            574..>1869
                     /locus_tag="VHE33_01860"
     CDS             574..>1869
                     /locus_tag="VHE33_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HVT96221.1"
                     /translation="MHRAAPAVLCLALSVSAAAQSPAPAKPPAGHPLTFSIYERARND
                     TWQWFAAPPASETYSYLQSLLRVSLSQRLAHWDWQAELDQAAVLHAPADAIAPIAAQG
                     QLGLGGTYYAANGNNTDPAAASFRQGFLRYDAAPDKNLRLGRMEWVEGVELHPKDTTV
                     LWLQNNRVQQHLIGNFGFANAQRSLDGIDAHYGSGDWDITALAARADQGVFNMNVNPE
                     LNVDTQYLAYSRLANRGRVLARAFAIGYHDGRTGITKTDNRPAGVRATDHKNIRIGSY
                     GGDLIATQPAGRGVLDFVFWGAYQNGAWGRLTQSAGAAQLEAGYRFTKVGTQPWFRGG
                     WWRSTGDNNNTDSKNNTFFVVLPTPRVYARLPFYNMMNSTDSFIQLMDKPRKNWDLRS
                     DLHWLQLTSSHDLWYSGGGAFDNKVFGFTGKPANGHTSFA"
BASE COUNT          387 a          664 c          462 g          356 t
ORIGIN      
        1 ccacccccgt gactgaggcc tgattttgag ccaaaaccgg caacggaaga accagcagag
       61 tcaacagggc aaaccgaatg aaattccgca tgtgtacgac actccttatt tttctggcag
      121 aaagctgcaa agctctgaat tttctgtgaa atacgaaaga atgcacgttc aaaaccaaac
      181 ggaacgccgg ttaaaccctc tcttttcatc accaacaaat ggaatttcat ggcggtcact
      241 tccgcttaat cagagataaa tcccaaaaac tgtaaattcg gtcgctgccc cgggcttctg
      301 ctcctttttt gaggcacttt tcggcgtcgg gcgccctccc ctggcaggac gtcacgcccc
      361 cgccgggcaa tccaaaaaat tgtcgccagc cccggatttc tgaaaccgca ttcaactcaa
      421 aacagaagtc ttatgcgccg accttcccaa acgttggtca ccattcatct ctcgcgaacc
      481 ggtcctgttc aacgcccatc cgccagtctt actcacaaat ccatctcagg ttggttgtca
      541 gcctcgttag cttcaggcat ccttgcccgg atcatgcaca gagctgcccc agccgttctc
      601 tgcctcgccc ttagtgtctc cgcggcggct caaagccccg cgccagccaa accgccggcc
      661 ggccacccgc tcaccttctc gatctacgag cgcgcccgca atgacacctg gcaatggttc
      721 gcggctccgc cggcttccga gacgtattcc tatcttcaat cgctgctccg cgtgagcctc
      781 tcccagcgcc tcgctcactg ggactggcaa gccgaattgg atcaggcggc agtcctccac
      841 gctccggccg atgccattgc accgatcgcc gcccagggcc agctcggtct cggcggcacc
      901 tactatgcgg cgaacggcaa caataccgat cctgccgccg cctccttcag gcagggcttc
      961 ctccgctacg acgcggcacc cgacaagaat cttcgcctcg gccgcatgga atgggtcgaa
     1021 ggcgttgagc ttcaccccaa agacacgacg gttctgtggc tccagaacaa ccgcgtccag
     1081 cagcacctga tcggcaactt cggcttcgcc aacgcccagc gcagcctcga cggcatcgac
     1141 gcccactacg gctccggcga ctgggatatc accgcgctgg ctgcccgtgc cgatcagggc
     1201 gtcttcaaca tgaatgtcaa tcccgagctg aatgtcgaca cgcagtatct ggcctactct
     1261 cggctcgcca accgcggtcg cgttctggcc cgcgccttcg ccatcggtta tcacgacggc
     1321 cgcaccggca tcacgaagac cgacaaccgc cccgcagggg tgcgcgccac cgaccacaag
     1381 aacatccgca tcggcagcta cggcggcgac ctgatcgcga cccaacccgc cggacgcggc
     1441 gtcctcgact tcgttttctg gggcgcgtat cagaacggag cctggggccg tctgactcaa
     1501 agcgccggcg ccgcgcagct cgaagccggc taccgcttca ccaaagttgg aacgcagcct
     1561 tggtttcgcg gcggatggtg gcgttccact ggcgacaaca acaataccga ctcaaagaac
     1621 aacaccttct tcgtggtgct tcccacgcct cgcgtctacg cccggctgcc cttctacaac
     1681 atgatgaatt caaccgactc gttcattcag ctgatggaca agccgcgcaa aaactgggat
     1741 ctccgcagcg atctccactg gctccagctc acttcgagcc acgatctctg gtactccggc
     1801 ggcggcgcct tcgacaacaa ggtcttcggc ttcaccggca agcctgccaa cggccacacg
     1861 tccttcgcc
//
LOCUS       DASZYN010000070        13313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_838815, whole genome shotgun sequence.
ACCESSION   DASZYN010000070 DASZYN010000000
VERSION     DASZYN010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 13313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13313
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838815"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VHE33_01865"
     CDS             complement(<1..312)
                     /locus_tag="VHE33_01865"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVT96222.1"
                     /translation="MQARMNHPVMIFPDAMKAMFELKAVTEKSGLPQSTVQLVHLRAS
                     QINGCSVCIEMHADQLRQSGESLERVLTVAAWRDAPYFTDAERAALELTESMTRLSDR
                     AD"
     gene            complement(397..1005)
                     /locus_tag="VHE33_01870"
     CDS             complement(397..1005)
                     /locus_tag="VHE33_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVT96223.1"
                     /translation="MLAEMKEPDLTFAGLDEPLLAPIEKPRGLTMKLVYAMSRRQFGK
                     VMTPLKVFAARMPLGFGKFVGKVGTLDKQLTLPRELVMLIREQVAHLNVCLFCIDIGR
                     SFTIRASMNQAKFDALAEYATNPLFSEAERAALDYVTQLTRNKRVDAEAFARMKRHYS
                     ERQICEIVWLVATEHLYNISNIGLNIHSDMLCDIAKKGRATA"
     gene            complement(1034..1474)
                     /locus_tag="VHE33_01875"
     CDS             complement(1034..1474)
                     /locus_tag="VHE33_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HVT96224.1"
                     /translation="MPARNQSRSRLIVYWVTTLLVAAEMTLGGIWDLLRTQYVRAIMD
                     HLGYPGYLLTILGVWKLLGTAAILMPRFPRLREWAYAGMFFLYSGAVASHFFMGDGPA
                     RWAGPLVFCGFLMVSWRLLPAPWALRDPAGARAVTPARPHPSAL"
     gene            1547..2431
                     /locus_tag="VHE33_01880"
     CDS             1547..2431
                     /locus_tag="VHE33_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVT96225.1"
                     /translation="MEQRDFLAEQFEANRGHLRGVAYRMLGSLTDADDAVQESWLRLS
                     RNGAEGIDNLGGWLTTVVARVCLDMLRSRKSKREEPLEIPGTIDPVPQPHAKTDPETE
                     TMMADSVGLALLVVLDRLDPAERLAFVLHDMFDLPFDEIAGIVGRSEAATRQLASRAR
                     RRVRGVDVSETNTIGKQRRVVDAFLAALRRGDVPGLVALLDPDFSVRADATAVAALPP
                     DVQGAEAWAKHAVQTARGAKFAQPALVDGSVSIVIAPKGRLFRVLRLTIEGDRIAAME
                     VIGDPERLRQMDLAVFSD"
     gene            2739..3239
                     /gene="hoxE"
                     /locus_tag="VHE33_01885"
     CDS             2739..3239
                     /gene="hoxE"
                     /locus_tag="VHE33_01885"
                     /inference="COORDINATES: protein motif:HMM:NF005747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bidirectional hydrogenase complex protein HoxE"
                     /protein_id="HVT96226.1"
                     /translation="MPLYAERPPLPSDDKRWRIVNGTMRKHGYSRNALIEALHTVQSS
                     FGFLDEPAIKFVAQSLRVPLSQAYGVVTFYHFFSLKPPGKHTCTICTGTACYIKGSDK
                     LVAQTEKLLHIHPGQTTPDGEVSFTTARCVGACGRAPVVLLDGELSGQIEAAELEHQL
                     ERWTTQ"
     gene            3236..4879
                     /locus_tag="VHE33_01890"
     CDS             3236..4879
                     /locus_tag="VHE33_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NuoF family protein"
                     /protein_id="HVT96227.1"
                     /translation="MNVEELQEIAEAAVEERDKFEYQINVCMGTGCLSQHSDKVKEAI
                     EKEAGAAGKKCHVRRTGCMGLCAAGPLVLVEPSEQRYGHVHAADAKDVVASLGGEPIA
                     RLDPHLEGYFEGQKHIVLENSGHIDPEKIDEYIARGGYEALLKALTEMTPQTVIEQIS
                     KSGLRGRGGGGYPTGLKWDTVAKVKGAQKYVICNGDEGDPGAFMDRSVLESDPQRVIE
                     GMAIAAYAIGASKGYIYVRAEYPLAVQRLTVALQQARRRGFLGNAICETQFSFDIDIR
                     LGAGAFVCGEETALIASIEGKRGTPRPRPPYPAVSGLWGNPTLINNVETFANVAPVIR
                     HGGEWFSSMGTARSKGTKVFALTGKIKRTGLVEVPMGMTLRQIIFDIGGGVPDGRKFK
                     AVQTGGPSGGCLPEEHLDLSISYDALISAGSMMGSGGMIVMDDTSCMVSVARFFMEFC
                     MTESCGKCIPCRAGTVQMYELLTKICQGEATHRDLDQLTELCEVVGATSLCGLGQTAP
                     NPVLSTLRYFKHEYLAHIDEKRCPAGVCECSPVAVEVHA"
     gene            4876..5592
                     /gene="hoxU"
                     /locus_tag="VHE33_01895"
     CDS             4876..5592
                     /gene="hoxU"
                     /locus_tag="VHE33_01895"
                     /inference="COORDINATES: protein motif:HMM:NF005745.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bidirectional hydrogenase complex protein HoxU"
                     /protein_id="HVT96228.1"
                     /translation="MSAEPNVRTLTINGEEISARSDQSILDAAREHNIDIPTLCHLEG
                     LSEVGACRLCLVEVKGARRLLPACVTQVQEGMEVTTHSPKIDRYRTMILELYFAERNH
                     VCSVCVANGNCELQSLSLKLGMTHVEIPYQYPQLPVDASHPRFTVDHNRCVLCTRCVR
                     VCSEIEGANTWGVMGRGGESLVITDLKQPWGDSETCTSCGKCVQVCPTGALFEKTSVG
                     NKTRKHPDFLPYLQIMREGE"
     gene            5589..6122
                     /locus_tag="VHE33_01900"
     CDS             5589..6122
                     /locus_tag="VHE33_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96229.1"
                     /translation="MSDLKTLATVWLDGCSGCHMSLLDMDERLIELSSQFCLVYSPLV
                     DRKTFPDLVDVTLVEGAVASEDDLKKIRKIREHTRILVSLGDCALTGNVSAMRNACGP
                     QPVFVRVYEQNADLQKQVPTVGVPPLLARVRPVHEVVPVDVFVPGCPPSADTIYQVLS
                     QLLAGQVPTLGGVAHFG"
     gene            6172..7596
                     /locus_tag="VHE33_01905"
     CDS             6172..7596
                     /locus_tag="VHE33_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HVT96230.1"
                     /translation="MPRTIVIDPVTRLEGHGKITLQLDEAGIVQDATFHVTQLRGFEK
                     FTEGRPFYEMPNITARICGICPVSHLVASSKACDAILAVKIPPVAADLRRILNLAQLI
                     QSHALSFFYLSSPDLLLGMDAPAEQRSILHVAHTHPAIGADGVALRKFGQQIIEMLAG
                     RRIHPGWTVPGGVNEPLSVEKRDQMLAMIPAAKAAIQRTLQWYKGATEQFAAEIGVFA
                     NFPTLFMGLVDEHGLAEYYDGALRIIDSEGNIIAGELNPAQYSDFIGEAVEPYTYMKF
                     PYYKPLGYPGGTYRVGPLARLNLADHCDTPLADGEWREFRAMSPRPLLSSFHYHHARL
                     VEMLYATEKIESLLADKTILDKHVRATASVNSLEGIGVAEAPRGTLIHHYKVDENGLI
                     VWANLIIATGHNNAAMNRGILQTARHYVQGDQIADGMLNRVEAVVRTFDPCLSCSTHA
                     MGRMPLSVELRAPDGSLVKEVRRG"
     gene            7601..8095
                     /locus_tag="VHE33_01910"
     CDS             7601..8095
                     /locus_tag="VHE33_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HVT96231.1"
                     /translation="MKPVVKMNAAPATPPPALLLACGNSLRQDDGVGLRIAEAAEHLF
                     PASRLHIVAAQQFTPEMAADLAATDLVIFVDASAVDEPGAIRVVPVTPQADAPDTHRL
                     DPPALLAMAQTFCGHAPARAFALTVGACRFGYGEELSGPLRQAVPRAVRLLRSLVTAF
                     SARA"
     gene            8189..9154
                     /locus_tag="VHE33_01915"
     CDS             8189..9154
                     /locus_tag="VHE33_01915"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HVT96232.1"
                     /translation="MKAIVYRHFGSPDVLHCEETANPEPREGQVLIRVRAVGLNPLDW
                     KLMKGKPWPLRLIMGLHKPKHPGVDVAGEVESIGPGVAQFKSGDQVFGACRGALADYV
                     CAPASTLAIKPDNVTFEQAASVPVAAWTALQGLRDKARIRSGETVLINGAAGGVGTFA
                     VQFAKHFGAGVTGVCSTHNLELVRSIGADRVLDYTAQGLAEDDRQYDVILECVGSLSE
                     PQIRRMLTPKGRCVMIGASPNVSLTTILAGMLKMLVMAPFRKQKIVTFMARRSQNDLN
                     FIAGLLESGAVRPVIDRCYSMNEVAEAMSYLEAGHVRGKLVVHIA"
     gene            complement(9191..10723)
                     /locus_tag="VHE33_01920"
     CDS             complement(9191..10723)
                     /locus_tag="VHE33_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVT96233.1"
                     /translation="MDQNGRRIVIIGGGIAGLCAAVYAQRCGYETEVLEMHAMTGGLA
                     MSWHRQGYTFETCLHWLVGSKPGGDFNHVWREVCDIDQLQFVFPAEMARIEDEHGEGL
                     PIATNVDRLEKELLQRAPQDAKAIRSLAHDIRVFSKFRMPNPNKSWLMTLPTYLRNVP
                     LVPALRRLTKMSGYEYSKRFRNPLLQSFFGAGEMGQMCALALVFSLAWHNAGEGGYSI
                     GGSQALIHLIEKKLTSLGGHVRCGAKVERILVENDRAVGVRLSNGEMITADWVISAAD
                     GHATIYEMLGGRYRNAAIDRNYEKMPIFPSYLQVSFGVGRDLSDMPWAVSRILDEPIR
                     VDPETEMKQIGFRIFHFDPTFAPPGKTAVTSLLMTRNFRYWSDLYRDDPEQYRAEKDR
                     VADVVLRALERKIPGIREAVEVVDVSTPATVMRCTGNWKGSMEGWFLPPGVRFRALPN
                     KLPGLQRFRMVGQWISPGGGLPAGLMTARPAIQAICKEDHVAFLPVLKTEEKPHWRLR
                     TP"
     gene            complement(10748..11158)
                     /locus_tag="VHE33_01925"
     CDS             complement(10748..11158)
                     /locus_tag="VHE33_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96234.1"
                     /translation="MYVRVLAVVALLFLSITALYGGTVLIIHAHGNPWGMMPLNLLRD
                     SPFHSWLVPGIILLTANGVLGLWVLGLVLARCRHDGFWAMFQGCVLLGWLVAECVLLR
                     MVIWPHYLYGVVALVLILAGAAMRHSSPSGASAN"
     gene            11530..12774
                     /locus_tag="VHE33_01930"
     CDS             11530..12774
                     /locus_tag="VHE33_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96235.1"
                     /translation="MKRSLTLVVCLGAMIGFASYVVASASAQTLSPRNNVVAYVYVAN
                     SAGGNATEISAFAADHAGRLRPVSGSPFMENAGSMAVNGTYLMAVSGTNINAYRIADD
                     GSLTYAVSTGYQQVQPGCGGANQLEFDHTGQSLYVTEYQVDCSNNGLTNWAVDNSTGA
                     LNYLGLTDTGNWNVNAPSFIGNNVFAYTAFNDNCMYYSLNSFKRDANGMLTEFSSGLN
                     SPPPPAGFSGYIPYLGAADPTNHVAFAEIPANPPGCAADPVQLATYTVNSDGSLTTTS
                     TAANMPATAIQNIYDMKMAPSGQLLAVAGQEGLQIFHFNGANPITPYTGLLTTDPINQ
                     MFWDNDNHLYAITAAYSGGSNNLHVFTVTPWGYREAPGSPYTLSGPRNIIVQPWPRYQ
                     AWQPEPRVAGAIQRLGPLMLKP"
     gene            complement(12771..>13313)
                     /locus_tag="VHE33_01935"
     CDS             complement(12771..>13313)
                     /locus_tag="VHE33_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96236.1"
                     /translation="GSQVTFSMNSDPSGRQFQLSIAAKGKTTNNTFDRHQHTALLADL
                     DPAAYTAMAHFALAHAPTTWIVIPKQTGLLIPAQYNAQPDVKGTFHGQPVDAHHTSVV
                     ISEENGFSVELYYTSEGALLEADLPEQNFYVVRDDFKLQNRPHYEPPRGQAPPPDEGQ
                     PPENPAPEASPQPQALLRSY"
BASE COUNT         2627 a         4496 c         3727 g         2463 t
ORIGIN      
        1 gtcggcccga tcgctgagcc gcgtcatcga ttccgtcaac tccagcgcgg cgcgctccgc
       61 atccgtaaag taaggagcgt cacgccacgc ggctacggtc agaacccgct ccaggctctc
      121 gcccgactgc cgcaactggt ctgcgtgcat ttcgatgcac acgctgcatc cgttgatctg
      181 gctggcgcgc aggtgaacca actgaacggt ggactgcggc agcccactct tctcggtaac
      241 cgctttgagt tcgaacatag ccttcatcgc atcgggaaag atcatgacgg gatgattcat
      301 tcgtgcctgc atacagaaac ctcctgggaa tcaaaaatcg ctttcgtctt tcatggcgat
      361 gacgaatcgc cacctggcaa tgtgacgaat gctggcctac gccgtggctc ggcccttttt
      421 cgcgatgtcg cacagcatgt ccgagtggat gttcagtccg atgttgctta tgttgtagag
      481 gtgctcggtc gccaccagcc aaacgatttc gcaaatctgc cgttcgctgt agtggcgctt
      541 catccgggca aacgcttccg catcgacgcg cttattccgg gtcagttgtg ttacatagtc
      601 gagcgcggcg cgctccgcct cggaaaacag cgggttcgtg gcgtactccg ccagtgcgtc
      661 gaatttcgcc tgattcatcg aggcccgaat cgtgaacgag cggccgatat cgatgcagaa
      721 caggcacaca ttgagatgcg ccacctgctc ccggatcagc atcaccagtt cccgaggaag
      781 cgtgagctgc ttgtcgagcg tgcccacttt gccaacaaac ttcccgaagc ccagcggcat
      841 ccgcgccgcg aacaccttca gcggcgtcat gaccttgccg aattgccgcc gagacatcgc
      901 gtagaccagc ttcatcgtca gtccgcgcgg cttttcgatc ggcgccagca gcggctcgtc
      961 cagtccggca aaagtaagat caggctcctt catctctgcg agcatggagt cctccattct
     1021 ttaaagagtc tcgtcacaag gctgacggat gcggacgcgc cggcgtgaca gcccgcgcgc
     1081 cggcgggatc acgcagtgcc cacggcgcag gcagcagccg ccacgacacc atcagaaacc
     1141 cgcaaaagac cagcggtccg gcccagcgag cgggaccatc gcccatgaaa aaatgcgatg
     1201 ccacggctcc cgaatacagg aagaacatgc ccgcatacgc ccactcccgg agccgcggaa
     1261 accgcggcat gagtattgcg gccgtcccca gcagtttcca gactccaaga atcgtcagca
     1321 agtatccggg atagccaaga tgatccatga tggcgcgaac atattgcgtg cgcagaagat
     1381 cccagatgcc tcccagcgtc atctccgcgg cgacgagcag agtggtgacc cagtagacga
     1441 tcaggcggga tcgagattgg ttcctggcag gcatagagct ccttgtcaca accggcgggt
     1501 ccggttcgtc attaccatga cgaaagaacc cgggagaatg tgacagatgg agcagcggga
     1561 ttttctggcg gaacagtttg aggcaaatcg gggacacctg cgcggcgtag cctaccgcat
     1621 gctcggttca ttgaccgatg ccgacgatgc ggtgcaggaa tcatggctcc gcctgagccg
     1681 caatggcgcc gagggcatcg acaacctcgg tggatggctg accaccgtcg tcgcccgcgt
     1741 ctgcctcgat atgcttcgct cgcgtaagtc gaaacgcgaa gagccgctgg agattccagg
     1801 aacgatcgat cccgtacccc agccgcatgc gaaaaccgat ccggaaaccg aaaccatgat
     1861 ggcggactcc gtcggcctcg cgttgctcgt tgtcctcgat cgcctcgatc ccgctgaacg
     1921 cctcgccttc gtcctgcacg acatgttcga tcttcccttc gatgagatcg ccggcatcgt
     1981 gggacgatcc gaagcagcca cgcgccagct cgccagccgt gcgcggcgtc gtgtccgcgg
     2041 cgtcgacgtc tcggaaacaa ataccatcgg gaagcagcgc cgcgtggtcg acgccttcct
     2101 ggccgcactg cgccgcggcg atgtcccagg gctggtcgct ctgctcgatc ccgatttctc
     2161 cgtgcgtgcc gacgcgacgg ctgtcgctgc ccttcctccc gacgttcaag gcgcagaagc
     2221 ctgggcgaag catgcagtcc agactgcgcg cggtgcaaag ttcgcccaac cggcgctggt
     2281 ggatggcagc gtaagcatcg tgatcgcacc caaaggccgg ctcttccgtg tcctgcgcct
     2341 caccatcgaa ggcgacagga tcgcggcgat ggaggtcatc ggcgatccgg agcgcctgcg
     2401 gcagatggat ctggccgtgt ttagcgactg aagccacacc caactcgcct gcaaatcgac
     2461 gccaaattct ggcgtctcgt gcggttttcc tcaggaatcc acgcgattta acaaagggct
     2521 cccttgcccg cagtgatgat tgtcaccgac aatcgtccac atctgtccgc attctgtcct
     2581 gtaccccgcg ctggttcgcc ggatcgtcca ggctctgctc ctcccggcaa aacagcgcgt
     2641 cgaaaactcg tctccgcgtt cgcttcgttc acgctgaagc agcggcagga tcatgaaccc
     2701 gagccgcgat tgccggacag gcaatcgggg agaacacgat gccgctctat gcagaacgcc
     2761 cgcctctgcc ctccgatgac aagcgctgga gaatcgtcaa tggcaccatg cgcaagcatg
     2821 gctactcgcg caacgcgctg attgaagccc tgcacaccgt gcagtcctcc tttggctttc
     2881 tcgacgagcc ggccatcaaa tttgtcgcgc agtcgctccg tgttcctttg agccaggcgt
     2941 atggagtggt caccttctat cacttcttca gcctcaagcc gcccggcaag cacacctgca
     3001 ccatctgcac cggcactgcc tgctacatca aggggtcgga caagctcgtc gcgcagacgg
     3061 aaaagctgct gcatattcat cccggacaaa ccacgcccga cggtgaagtc tccttcacca
     3121 ccgcgcgctg cgtcggcgcc tgcggccgcg cccccgtggt tctgctcgat ggcgaactct
     3181 ccggacaaat cgaagccgcc gaactcgaac atcagctgga aaggtggacg acgcaatgaa
     3241 cgttgaggag cttcaggaga ttgccgaggc cgccgtcgaa gaacgcgaca aattcgagta
     3301 tcagatcaac gtttgcatgg gcaccggctg cctctcgcag cacagcgaca aggtcaaaga
     3361 agcgattgag aaggaagcag gcgccgccgg aaaaaagtgc cacgtccgcc gcaccggatg
     3421 tatgggcctc tgcgcggcag gccccctggt gctggtcgag ccttccgagc agcgctacgg
     3481 ccacgtgcac gccgccgatg cgaaggacgt cgtcgcctcg ctcggcggtg agccgattgc
     3541 gcgcctcgac ccgcacctcg aaggctactt cgagggccag aaacacatcg tgctcgaaaa
     3601 ctccggccac atcgatccgg agaaaattga cgaatacatc gcgcgcggtg gctatgaagc
     3661 tctcctgaag gcgctcacgg agatgacgcc gcaaacggtc atcgagcaaa tctccaaaag
     3721 cggtctgcgc ggacgcggag gaggcggcta tccaaccggc ctgaaatggg acacagtcgc
     3781 caaggtcaaa ggcgcgcaga aatacgtcat ctgcaacggc gacgaaggcg accccggcgc
     3841 attcatggat cgcagcgtgc tcgagagcga tccgcagcgc gtcatcgaag gcatggcgat
     3901 tgccgcttac gccatcggcg ccagcaaggg ttacatctat gtccgagcag agtacccgct
     3961 cgccgtgcag cggttgaccg tcgctctgca gcaggcgcgc cgccgcggtt tcctcggcaa
     4021 tgcgatctgc gagacccagt tcagcttcga catcgacatt cgcctcggcg ccggagcctt
     4081 tgtctgcggc gaagagactg cgctcatcgc ctccatcgaa ggcaagcgcg gaacgcctcg
     4141 cccgcgtccg ccctatcccg cagtttcagg actctggggc aatcccacgc tcatcaataa
     4201 cgtcgaaacc tttgccaacg tcgcgcccgt cattcgccac ggcggcgagt ggttctcctc
     4261 gatgggtaca gcacggagca aaggcaccaa ggtctttgcg ctcaccggca aaatcaagcg
     4321 caccggactc gtcgaagtgc ccatgggcat gacgctgcgc cagatcatct tcgacattgg
     4381 cggcggcgtt cccgacggca gaaaattcaa ggcagtacag actggcggac cctcgggcgg
     4441 ctgtttgccc gaggaacacc tcgatttgtc catcagctac gacgccctca tcagcgccgg
     4501 ttccatgatg ggttcgggcg gcatgatcgt catggacgat acctcgtgca tggtctcggt
     4561 cgcccgcttc tttatggaat tctgcatgac cgaatcctgc ggcaaatgta ttccgtgccg
     4621 cgccggcacc gtccagatgt acgaattgct cacgaagatc tgccagggcg aggcgaccca
     4681 ccgcgatctc gaccagctga ccgagctatg cgaggtcgtc ggcgccacca gcctctgcgg
     4741 cctcggccaa acggcgccca accccgtgct cagcacgctg cgttacttca aacatgagta
     4801 cctggcgcac atcgacgaaa agcgatgccc tgcgggagtc tgcgagtgca gtcctgtggc
     4861 agtggaggtg cacgcatgag cgcggaacca aatgttcgca cactgaccat caacggcgaa
     4921 gagatcagcg cccgctccga ccagtccatc ctggacgccg cgcgcgagca taacatcgac
     4981 attcctaccc tttgccatct cgaagggctc tccgaagtcg gtgcctgccg tctttgcctc
     5041 gtcgaagtca aaggtgcgcg tcgtttgctg cccgcatgcg tcacgcaggt gcaggaaggc
     5101 atggaagtca ccacgcactc tccgaagatc gaccgctatc gcaccatgat cctcgagctg
     5161 tactttgccg agcgcaacca cgtctgctca gtctgcgtgg ccaacggcaa ttgcgagttg
     5221 cagagcctgt cgctcaagct cggcatgacc cacgtcgaga ttccttacca gtacccacag
     5281 ttgcccgttg acgcgtcaca cccgcgcttc accgtcgacc acaaccgctg cgtgctctgt
     5341 actcgctgcg tgcgtgtctg ctctgaaatc gaaggcgcca acacctgggg cgtgatgggc
     5401 cgcggcggcg agtccctggt gattaccgat ctgaaacagc cgtggggcga ttcggaaacc
     5461 tgcaccagct gcggcaaatg cgtgcaggtg tgccccaccg gcgcgctctt tgagaagacc
     5521 agcgtcggca acaaaacccg taagcatccg gatttcctgc cgtacctgca gatcatgcgg
     5581 gagggagaat gagcgacctg aaaacactcg ccaccgtctg gctcgatggc tgctccggct
     5641 gccacatgtc tttgcttgac atggacgagc ggctcatcga gctcagcagc cagttttgcc
     5701 tggtctacag cccgctggtc gatcgcaaaa cctttcccga cctcgtcgat gtcacgctgg
     5761 tcgaaggcgc cgtcgccagc gaagacgacc tgaagaagat tcgcaaaatc cgcgagcaca
     5821 cccgcatcct cgtctcgctc ggcgactgcg cgctcaccgg caacgtctcc gcgatgcgca
     5881 atgcctgcgg accgcagccc gtcttcgtcc gcgtctacga gcagaacgcc gatctgcaga
     5941 agcaggtgcc caccgtcggc gttccgccgc tgctcgctcg cgtgcgcccg gtgcacgaag
     6001 tcgtgcccgt cgacgtcttc gtccccggct gcccaccgtc ggcggacacg atttaccagg
     6061 tgctgtcgca gttgctggca ggtcaggttc ccacgctggg cggcgtcgcc cactttggct
     6121 gaacgagagc cctggagcag gaacagaagc cagggaaagg aagcaggaaa aatgccccgt
     6181 accatcgtca tcgatcccgt cacacgcctc gaaggacacg gcaagatcac cctgcagctc
     6241 gacgaagccg gcatcgtgca ggacgcgacc tttcacgtga ctcagctgcg cggcttcgag
     6301 aaattcaccg agggccggcc cttctacgag atgcccaata tcacggcgcg catctgcggc
     6361 atctgcccgg tcagccatct cgtcgcctcg tccaaagcct gtgatgccat tctcgccgtc
     6421 aaaatcccac ctgtcgccgc cgacctgcgc cgcatcctga atctggccca gctcatccag
     6481 tcgcacgcgc tcagcttctt ctatctgtcc tcccccgatc tgctcctcgg catggatgcc
     6541 cctgccgagc aacgcagcat cctgcacgtc gcccatacgc acccagccat cggcgccgac
     6601 ggcgtggcgc ttcgcaaatt cggccagcag atcatcgaaa tgctcgccgg acgccgcatt
     6661 caccctggat ggaccgtgcc cggcggcgtg aacgagccgc tcagcgtgga gaaacgcgac
     6721 cagatgctcg ccatgattcc tgccgcgaag gccgccatcc agcgcaccct gcagtggtac
     6781 aaaggcgcaa ccgagcagtt cgccgccgaa atcggtgtct tcgccaactt ccccacgctc
     6841 ttcatgggcc tggtcgacga gcacggcctg gccgagtact acgacggcgc gctgcgcatc
     6901 atcgactcgg aaggcaacat cattgccggc gagctgaacc ccgctcaata ctccgacttc
     6961 atcggtgaag cggtcgagcc ctacacctac atgaagttcc cctactacaa gccgctcggc
     7021 taccccggtg gaacctatcg cgtcggtccc ctggctcgcc tcaaccttgc cgaccactgc
     7081 gatacacctc tggccgacgg ggaatggcgc gagttccgcg cgatgtcgcc ccggcccctg
     7141 ctcagctcct tccactatca ccacgcgcgg ctcgtggaaa tgctgtacgc gaccgaaaag
     7201 atcgaatcgc tgctggccga caaaacaatc ctcgataagc acgtgcgcgc taccgcctcg
     7261 gtcaacagcc tcgaaggcat cggcgttgcg gaagcgccgc gtggcacgct gatccaccac
     7321 tataaggttg acgagaacgg cctcatcgtc tgggccaacc tcatcatcgc caccggccac
     7381 aacaatgccg ccatgaaccg cggcatcctg caaactgccc gccattacgt acagggcgac
     7441 cagatcgccg acggcatgct gaaccgcgtc gaagccgtgg tccgcacctt cgatccatgc
     7501 ctcagttgct ccacccacgc catgggccgg atgccgctct ccgtcgaact gcgcgcgccc
     7561 gacggctccc tggtgaagga agtgcggcga ggctaacgcc atgaagcctg tcgtgaaaat
     7621 gaatgcggcg cccgcgaccc cacctcccgc gctgttgctc gcctgtggca actcgctccg
     7681 ccaggacgac ggcgtcggcc tgcgcatcgc tgaagccgcc gagcacctct ttcccgcctc
     7741 gcgcctgcac atcgtcgccg cccagcagtt caccccggaa atggccgctg acttagcagc
     7801 cacggacctc gtcatcttcg tcgatgcttc ggccgtcgac gagcccggag cgatccgcgt
     7861 tgttcctgtc actccgcagg ccgacgcgcc cgacacccac cggctcgatc cgccggccct
     7921 gctcgcgatg gcgcagacct tttgtggcca cgctcccgcg cgtgccttcg cgctcaccgt
     7981 tggcgcatgc cgcttcggct acggcgaaga gctgtccggc cctcttcgtc aggccgttcc
     8041 ccgtgctgtg cggctgctta ggagtttggt gactgccttt tctgcccggg catagtcaac
     8101 cccggcgccc cgactgcgca cagcgtgaca ggcgttactg acaactgcgc cgtcgagggc
     8161 tatccttgcg ccgactcgag gcgactgcat gaaagccatt gtctatcgcc acttcggttc
     8221 ccccgacgtt ctccactgtg aagaaacagc gaatcccgag ccgcgagaag gccaggttct
     8281 catccgggtt cgcgccgtgg gcctcaatcc gctcgactgg aagctgatga agggcaaacc
     8341 ctggcctctt cgcctgatca tgggcctgca taaaccgaaa catccaggcg tcgacgttgc
     8401 cggcgaggtg gaatccatcg gtccaggtgt ggcgcagttc aagtctggcg atcaggtctt
     8461 cggtgcctgc cgaggcgcgc tggcggacta cgtctgcgcg cctgcatcga ccctcgccat
     8521 caagccagac aatgtcacct tcgagcaagc ggcttccgtt cctgtcgcgg cctggaccgc
     8581 ccttcaggga ctgcgcgaca aagctcgcat ccggtcaggc gaaaccgtac tcattaacgg
     8641 ggcagcgggt ggtgtcggca cctttgccgt gcagttcgcg aaacatttcg gcgccggcgt
     8701 gaccggtgtc tgcagcacgc acaacctgga actggtgcgc tccatcggag ccgatcgtgt
     8761 cctcgactac accgcgcagg ggctcgccga agacgatcgg cagtacgacg tgatcctcga
     8821 atgcgtgggc agtctctccg aaccccagat acgccgcatg ctgacgccga aaggcagatg
     8881 cgtgatgatc ggagcgagcc ccaacgtgag cctcacgacg attctcgcag gcatgctcaa
     8941 aatgctcgtg atggcgccct tccggaagca gaagatcgtc acgttcatgg ccaggcgcag
     9001 tcagaacgac ctcaacttca tcgccggact cctcgagtca ggcgccgtca ggcccgttat
     9061 cgaccgttgc tacagcatga acgaagtcgc cgaagccatg agctacctgg aagccggaca
     9121 cgtacgcggc aagctcgtcg tccacatcgc gtaggcgcgc cgcaccattc tgatatccgg
     9181 acccgcgacg ctacggtgtc cgcaatctcc agtgcggttt ctcctccgtt ttcagcaccg
     9241 gcaaaaacgc cacatgatcc tctttacaga tggcctgaat cgccggacgc gccgtcatca
     9301 atcccgccgg cagtccgccg ccgggggaaa tccactgtcc caccatcctg aaccgctgca
     9361 gtcctggaag tttgttcggc agtgcacgga atcggacgcc cggcggcaaa aaccagcctt
     9421 ccatgctgcc cttccagttg ccggtgcagc gcatcaccgt cgccggcgtc gacacatcca
     9481 caacctccac cgcctcgcgg attccaggaa tcttccgctc cagagccctg agaaccacat
     9541 ccgctacccg gtccttctcc gcgcgatact gctcgggatc gtcccggtac aaatcgctcc
     9601 agtaccgaaa atttcgcgtc atcagcaggc tcgtgacggc agtctttccc ggcggcgcga
     9661 acgtcggatc gaagtggaag atgcggaagc cgatctgctt catctccgtc tcagggtcca
     9721 cgcgaatcgg ctcatcgaga atccggctca ccgcccacgg catatccgac agatccctcc
     9781 ccacgccaaa cgagacctgc agatacgagg ggaaaatcgg catcttctca taattccgat
     9841 cgatcgccgc gttcctgtag cggccgccca gcatctcgta aatcgtggca tgtccgtcgg
     9901 ccgctgagat cacccagtcc gcggtaatca tctctccatt cgacagccgt acgccgacgg
     9961 ctcggtcgtt ctccaccaaa atgcgctcca ccttcgcgcc gcatcgcaca tgcccaccca
    10021 ggctcgtcag ctttttttca atcaaatgga tcagcgcctg cgagccgccg attgagtagc
    10081 ctccctcacc ggcgttgtgc cacgccagtg aaaagaccag ggccaacgcg cacatctgtc
    10141 ccatttcgcc cgccccaaag aagctctgca gcagcggatt tctgaagcgc ttgctgtact
    10201 cgtacccact catcttcgtc agccggcgca gtgctggcac cagcggaacg ttccgcagat
    10261 acgtgggcaa ggtcatcagc cagctcttgt tcgggttcgg catccggaat ttgctgaaca
    10321 cgcgaatgtc gtgcgccagc gagcgaatcg ccttcgcatc ttgcggcgcg cgctgcagca
    10381 gctccttctc cagccgatcg acgttcgttg ctatcggtaa accttccccg tgctcatcct
    10441 cgatccgcgc catctcggct ggaaacacaa attgcagctg gtcgatgtcg catacttcgc
    10501 gccacacgtg attgaagtcg ccgcccggct tcgagccaac cagccagtgc aggcaggttt
    10561 caaacgtgta cccttgccgg tgccagctca tcgccagccc gccggtcatg gcgtgcatct
    10621 cgagaacttc cgtctcatac ccgcagcgct gcgcataaac ggccgcgcaa agcccggcga
    10681 tgccgccgcc aatgatgacg atcctgcggc cattctggtc catagtgttg tctccttgct
    10741 ttgaccttca gttcgccgaa gcccctgagg gtgaactgtg gcgcatcgcg gctcccgcca
    10801 ggatcagaac cagtgccacc accccgtaga ggtaatgagg ccagatgacc atgcgcagca
    10861 gcacgcattc cgcaacgagc catcccagca gcacgcatcc ctggaacatc gcccagaacc
    10921 cgtcatgccg gcagcgcgcc agcaccagcc ccagaaccca aagtcccagt acgccgttcg
    10981 cagttagcag gatgatgccc ggaacgagcc acgagtgaaa cggcgagtct cgcagcaggt
    11041 tcagcggcat catgccccag gggttgccat gggcatgaat gatcaacacc gtcccgccgt
    11101 agagcgcggt tatcgacagg aacagcagag ccacgacggc cagcacgcga acgtacacaa
    11161 ccaatcctcc cctggcggag gaccgttccc tgcagattaa gccgcaccct ggccccgaga
    11221 atcggcggca accgtcatgc gagttcgggc cggtgccagt ctgcccttcg caagcgaagt
    11281 ataagtgcag ggcggcaatt cgtctgtgac ggcaggcaca ggtccctgcg actctcagcg
    11341 cacattacga tcgtctatct ctcttcccct tccgtaacga ggagcggtcc tccgccgaca
    11401 cggcggagtc gagggacaac cacccggcct ttaccaactt ctaacgacgt ggcaagcagt
    11461 gcccgcgcta tcctggcaag tctgcccggg tttcatccat cacaacaacc cttcatcagg
    11521 aggtctcccg tgaaacggag cttgacgctc gtcgtgtgtc ttggcgcgat gatcggattt
    11581 gccagttatg tcgttgcctc tgccagtgca caaacactat ccccccgcaa taatgtcgtt
    11641 gcctacgtct acgtggccaa ctccgccggc ggcaatgcca cagagatttc ggccttcgcg
    11701 gccgatcacg ccgggagact gcgccccgtc tccggctcgc ccttcatgga gaacgccggc
    11761 tccatggcgg tgaacggcac gtatctgatg gcggtatccg ggaccaatat caatgcctac
    11821 cgcatcgctg acgacggttc gctcacttac gcagtctcta ccggctatca gcaggtccag
    11881 ccaggatgcg gcggcgccaa tcagctcgaa ttcgatcaca ccggtcaatc cctgtatgtg
    11941 accgaatatc aggtcgactg ctccaacaac ggactcacca actgggccgt cgacaactca
    12001 accggagcgc tgaactacct tgggctgacc gataccggga actggaacgt caatgcccct
    12061 tctttcatcg ggaacaatgt gttcgcctac accgcgttca acgacaactg catgtactac
    12121 agcctgaatt cgtttaagcg cgacgcgaat ggaatgctga cggagtttag ctcaggctta
    12181 aattctccgc cgcctccggc aggattcagc ggctacatcc catatctggg ggctgccgat
    12241 cccaccaacc atgttgcctt cgctgagatc ccggcgaatc cgcccggctg cgcagctgat
    12301 ccggtgcaac tggcgacgta caccgttaat tctgacgggt cccttaccac caccagcacc
    12361 gcggccaata tgcctgcgac cgcgatccag aacatttacg acatgaagat ggcgccgtcc
    12421 ggtcaactcc ttgctgtggc tggacaggaa ggtctgcaga ttttccactt caacggtgcc
    12481 aaccccatca ccccctacac cggcctgctg accaccgatc ccatcaacca gatgttttgg
    12541 gacaacgaca atcatctcta cgcgatcacg gcagcttata gcggcggatc caacaacctt
    12601 catgtattca ccgttacgcc gtggggttat cgcgaggcgc caggttcgcc gtacacgctt
    12661 agcggtccgc gcaacatcat cgttcagccc tggccgcgct atcaggcctg gcagccagag
    12721 ccccgagtcg ccggcgcgat ccagcggctg ggcccgctta tgttgaagcc ttagtacgac
    12781 cgcagtaaag cctgcggctg gggagaagcc tccggcgccg ggttttccgg aggctgaccc
    12841 tcatccggcg ggggcgcctg tccgcgcggc ggctcgtagt gcggacgatt ctgcagcttg
    12901 aaatcgtcac ggaccacata gaaattctgc tccggcaaat cagcctcaag cagtgcacct
    12961 tcgctcgtgt agtaaagctc cacactgaag ccgttttctt cgctgataac cacgctcgtg
    13021 tggtgcgcat ccacgggttg gccgtggaac gtccccttca cgtccggttg cgcgttgtac
    13081 tgcgcaggaa tcagcaaccc ggtctgtttc gggattacga tccaggtcgt cggcgcatgc
    13141 gccagcgcaa agtgcgccat cgctgtgtac gccgcgggat caagatcggc cagcagagcc
    13201 gtgtgctgat gacggtcaaa ggtgttgttt gtcgttttgc ccttggcggc aatgctcagc
    13261 tggaactgcc gtcccgatgg atccgagttc atgctgaacg ttacctgcga tcc
//
LOCUS       DASZYN010000071         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_902808, whole genome shotgun sequence.
ACCESSION   DASZYN010000071 DASZYN010000000
VERSION     DASZYN010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902808"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VHE33_01940"
     CDS             <1..359
                     /locus_tag="VHE33_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96237.1"
                     /translation="SLVRPLQLLIKYSKFSMASKFQQFFGIERRRLRHVPGLHPEGTT
                     CIRQQSINTSMGRPLSPSLEYFKMPRALASCGHLRPVRPPTIIATALHRNPWREVITL
                     RNHKNDQHQQPLTDAE"
BASE COUNT          317 a          575 c          396 g          279 t
ORIGIN      
        1 gttcgctggt gcggccacta cagctcctca tcaaatattc caaattcagc atggcgtcga
       61 aatttcaaca atttttcggc atcgagcggc gacgtctccg acacgttcct ggccttcacc
      121 ctgaaggaac aacgtgtatt cgtcagcaat ccatcaatac atcgatgggg agaccacttt
      181 cgccctcgct cgaatatttc aagatgccac gcgctttagc gagctgcggt cacctccgcc
      241 ccgtccgccc tcccacgatc atcgccacgg ctcttcatcg caacccgtgg cgggaggtaa
      301 taaccctcag gaaccacaag aatgatcagc accaacaacc gttaacggac gcggaataac
      361 cctactcgca ggcaataacc ctactcggcc gtcgggcgca ctgaataacc ctaacgccgc
      421 cggcgacgcc acgcctcggc gcgccactcg ccggccgcgt aaccctaatg ccgtccacga
      481 gcgaataacc ctaacgggct ccgccgtgac gcccaggcag ctgctgcagt gcactaaccc
      541 taacgggccg cctcagcctc gcctggctcc ttccggccag cgcaaccaga cgcggcgtgc
      601 cttcgggctc ggcacgccgc ctctcaccgc cgctgcgcca tcacgtctat cacggtacgg
      661 ccgtcgctcg ctcgcgcact gcgagcatta gcgcggctta ttacttgtac cgtgccgccg
      721 taccggcgtg ctgtgcttcg cagcactgtg cgtgtgcgtg ccaggttcgt ctgaaaagcc
      781 acagcgtcgg gctcgtctcg gaggctcaac aaccctgctt tgggcctcgc ctcacagcgg
      841 tgctgtgcga gacgagacta cgctctaccg tggaagacgg ccgagcacac acgcgtgcca
      901 cgcctacgac ggaagacctc acccgagtct gttcgattca acctccaaga aatgaagtgc
      961 gtgcctcaat gtcggaccaa gtcgatacgg acaagacgca atgcaagacc agccaaggcg
     1021 acatctcgcc tgcggcactc tcaatgacta agcctatcta cctgctttat ttgcggctcc
     1081 gccgctgtca tttatttgcg cagctttttg ccagctgccg accgtttggc ctgcccgtca
     1141 ggccgcgctc gcccgctgcc tgcgccctct cttgccgcct gcgcgctgcc cgcttgaccg
     1201 ccgtccactc gcctgacgaa ggttccagtg tgaaccgaaa cgcgtagcga actccattgc
     1261 acacgcactt cctctcgcga gagcgcctgc gctcgcacgc gcgcgcgcac gtcaccgcgc
     1321 tgccgccgcg ctgcgcccgc acccgcttcc caccccgaac agaccacgca gcgcagccga
     1381 gtgccgcgac gtagcagcgc gcgctaaccc aagggttaac gcgccgctgc gcggcgcgcc
     1441 taataaccct aataacccta ataaccctca ggaaccacaa gaatgatcag caccaacaac
     1501 cgttaacgga cgcggaataa ccctactcgc aggcaataac cctactcggc cgtcgggcgc
     1561 actgaat
//
LOCUS       DASZYN010000072         5330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_838884, whole genome shotgun sequence.
ACCESSION   DASZYN010000072 DASZYN010000000
VERSION     DASZYN010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838884"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VHE33_01945"
     CDS             <1..190
                     /locus_tag="VHE33_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96238.1"
                     /translation="PEVYRSFGFPGADDLGNMFQFKRDFNDDFRKPRDVERSRRLNPE
                     LQNFDAWLTANKSRIPLS"
     gene            complement(207..1385)
                     /locus_tag="VHE33_01950"
     CDS             complement(207..1385)
                     /locus_tag="VHE33_01950"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVT96239.1"
                     /translation="MPAQVFPAAQVSNPTPPPELSQRWLPSSSLLVIAGAAPILLALP
                     FLAESLRMGLILEGSILAASGLVAPFLLWRLRIFREASASARSEHARFLAAAENTLDD
                     FYIFDGVPDSTGAIVDFRFSYINPNAERRLGVPRESLYGKILTEVRPFMIRSGLIEQY
                     REVVRTGAPYSCEVFIDDDMIQGTWLNVQVVKLGNGVAITSRDVTEHRRRSEHIHYLA
                     HHDHLTGLANRTLLYERLRLAIARAEELCQSVAIFVLDIDNFKQINDSLGHADGDLLL
                     ATVGQRLLASVRESDTVARLGGDEFVIVMPDFRSMEDVERCGRQIIRNAAQPLNIAER
                     SLSITVSVGIAIYPDAGLTAQDLLRSADAAMYAVKDSGRNSLRIYSEDMMDSRRPTLV
                     "
     gene            complement(1530..2078)
                     /locus_tag="VHE33_01955"
     CDS             complement(1530..2078)
                     /locus_tag="VHE33_01955"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 domain-containing protein"
                     /protein_id="HVT96240.1"
                     /translation="MALQFTTSYIEDSLALFAYYKKLAERAMDQVSDEQLFAAIDDDA
                     NSIAIIVKHMAGNMRSRWTDFLTTDGEKADRNRDSEFEDPPTSRADLLAMWERGWTCL
                     FSALQPLNDADLVRTVTIRGEKHSVMQAINRQIAHYPHHIGQIVLLAKHFCCGNWQTL
                     SVPRKGSAEFNRRVSAGEASQR"
     gene            complement(2121..2750)
                     /locus_tag="VHE33_01960"
     CDS             complement(2121..2750)
                     /locus_tag="VHE33_01960"
                     /EC_number="3.2.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
                     /protein_id="HVT96241.1"
                     /translation="MEAQTVYLYVFDGMADWEPAFAIAGINNPRFQRQPGRFCVATVA
                     SSRETITTMGGIHIEPDLALDDLFPTSSALLILPGGDAWERGGNRDAVEKVAEFLAVG
                     VPVAAICAATLALARAGFLDNRLHTSNSPEYLKATRYRGHSLYRNIPAIADGNLITAS
                     GAAPIDFAYQIFGMLEVYSEEALNAWYALFRYGDASKFEALADAVAQHS"
     gene            complement(2837..>5330)
                     /locus_tag="VHE33_01965"
     CDS             complement(2837..>5330)
                     /locus_tag="VHE33_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT96242.1"
                     /translation="FVLRDLKIAIRHLSKSSGFSITAVLMLALGIGATTAIFSIVEGV
                     LLRPLPFPHPQQLVVLTDTIQGADVGGNNEAGVTGPDIVNYTRDTHSFTALGGYTQPG
                     YELSGIGDPAQINAARMSPGVFAALGVQPMLGRTYTADEDAQHQQVAVLSYATWQSRF
                     HGESSILGQKILLDRKPYVVIGIMPRNFEFPLVPGHLNRSELWIPLSLRADELTGGAQ
                     ANWSYSMVGRLKDGVTPEQAREDANRVAQATMRNYPAFMASLHISPLVRGLHENTIAQ
                     ARPLIRTLFLAVAVVLLIACANLAGLLLVRAIRRRREIAVRLALGSSGRTLLRQALLE
                     SLVLSVGGGIIGLILAAIALKLGTSLLPETLPLIGSIGIDWMVATFALALAIVTGALC
                     GVAPAFAAIRTSVNETLKEGGRTGSSGAGHARLRSGLVIAEIAVALVLLAASGLLLRS
                     FEKMREADLGYRPDHTVVAAYSLPRKQYTTQPAVDQFNQELLRRVEGIPGVTSAGLTS
                     FLPANGTQGNSAFVAEGYVPAKAGAIDLATPIQIQGDLFHAMGIRLLRGRLLTDADNN
                     PKAQLVAVVNEEFARQSWPGQDPIGKRMRIGTTEMQTPWATVVGEVASMKEGSPDADA
                     RQQYYYENDQAETFFGSLGHPETDINGNGGYIVLRTSLPPEMIENQLRAAVRSLDPQL
                     PLTQLQTMEHAISDSEAPRRFNTVLISAFAAAAVLLAILGIYSVIAFTVAMRAQEMAI
                     RMALGSQRSGIVSLILFSALKLAGAGCVIGLVGALLASRLLKTFVFGVSTLDPLVLTL
                     AAVVVLMLALAASLLPARRAAAINPMQALRAE"
BASE COUNT         1031 a         1428 c         1926 g          945 t
ORIGIN      
        1 gccggaggtg tatcgcagct ttggatttcc gggagcggac gatctgggca acatgttcca
       61 gttcaagcgc gacttcaacg acgatttccg caagccgcgg gatgtggagc ggtcgcgccg
      121 gctgaatcct gaactgcaaa attttgacgc gtggctgacg gcgaacaaga gccggattcc
      181 actgagttga atcggagcgt accggctcat accagggtcg ggcggcggct gtccatcatg
      241 tcttctgaat agatgcggag actgttgcgg cccgagtctt tcactgcgta catggcagca
      301 tcggcggagc ggaggagatc ctgtgccgtg agcccggcgt cggggtaaat ggcgatgccg
      361 acgctgaccg tgatgctgag gctgcgctct gcgatgttga gcggttgagc cgcattgcgg
      421 atgatctgcc ggccgcatcg ctctacgtct tccatggagc ggaagtcggg catgacgatg
      481 acgaactcgt cgccgccgag gcgggcgacc gtatcggact ctcgcacgga agcgagcagg
      541 cgctggccca cggtagcgag cagcagatcg ccgtcggcgt ggccgagcga atcattgatc
      601 tgtttgaaat tgtcgatgtc gaggacgaag atggcgacgc tctggcagag ttcctcagcg
      661 cgcgcgattg caaggcggag acgctcgtaa agaagcgtgc ggttggcaag accggtgaga
      721 tggtcgtggt gggcgaggta atggatgtgc tcggagcggc ggcgatgctc cgtgacgtcg
      781 cgactggtga tggcgacacc gttgccgagt ttcacgacct gaacgttgag ccaggtgccc
      841 tggatcatgt cgtcgtcgat gaagacttcg cagctgtagg gagcgccagt gcggacgacc
      901 tcgcgatatt gctcgatgag gccggagcgg atcatgaagg ggcgcacctc ggtgaggatc
      961 ttcccgtaga ggctttcgcg cggcacaccg aggcggcgct cggcattggg gttgatatag
     1021 ctgaagcgaa aatcgacgat ggcgccggtc gagtccggca cgccgtcgaa gatgtagaag
     1081 tcgtcgaggg tattttctgc ggcggcgagg aagcgggcat gctcgctgcg cgcggaggcc
     1141 gaagcctcac ggaaaatgcg cagacgccac agaagaaatg gggcgaccag gccggaggca
     1201 gccagaatcg aaccttcgag gatcagcccc attcgcagcg attccgccag gaaaggaagc
     1261 gccagaagga tcggcgcggc gccggcgatg acgaggaggg aagaactggg gagccagcgc
     1321 tgcgagagct ctggtggcgg tgtggggttg gatacctgcg cagctgggaa gacttgggcg
     1381 ggcatattca gcgaatgagc cctgggtcgg gagctattga aggggattct atcgtgtcga
     1441 attaacaaac gggatagtac gaattggtta ttaacccgcc ccaagcgtgt cgcggggccg
     1501 tccgcgtcgc gcttcgcacc gtccgatggc tagcgctggc tggcctcgcc ggcggaaacc
     1561 ctgcgattga attctgcgga acctttgcgg ggaacactga gcgtttgcca gttgccgcag
     1621 cagaagtgtt tcgccagcag aacaatttga ccgatgtgat gcgggtagtg cgcgatctga
     1681 cgattgatcg cctgcatcac agagtgcttt tcgccgcgaa tggtgacggt gcggaccagg
     1741 tcggcatcgt tgaggggctg cagtgcggag aagagacacg tccagccacg ctcccacatt
     1801 gccaggaggt cggcgcgcga ggtgggagga tcttcgaatt cgctgtcgcg gttgcggtcg
     1861 gccttctcgc catcggtggt gaggaagtcc gtccagcggg agcgcatgtt cccggccatg
     1921 tgcttcacga tgatggcgat ggaattggcg tcgtcatcga tggcggcaaa gagctgctca
     1981 tcggaaacct gatccatggc acgttcggcg agcttcttgt aataggcaaa gagggcgagg
     2041 gagtcctcga tgtaagacgt ggtgaattgc agcgccatgg agagagtgta gtggagtggc
     2101 gcggcggcgg gtgttcggga ctagctgtgc tgggcgacag cgtcggccag cgcctcgaat
     2161 tttgaggcgt cgccgtaccg gaagagggca taccacgcgt tgagcgcttc ttcggaatag
     2221 acctcgagca tgccgaagat ctgataggcg aaatcgatgg gagcggcccc cgaggcggtg
     2281 atgaggttgc cgtcggcgat ggccgggatg ttgcgataga gcgagtggcc gcgataacga
     2341 gtggccttca ggtattcggg tgagttgctg gtgtggaggc ggttgtcgag gaaaccggcc
     2401 cgcgccaggg cgagagtggc cgcgcagatg gcggcaacgg gaactccaac cgcaagaaac
     2461 tcggccactt tttcaaccgc gtcccgattc ccgcctcttt cccatgcgtc gccgccggga
     2521 aggatgagga gggcactgga ggtgggaaag agatcgtcga gggcgaggtc aggctcgata
     2581 tgaataccgc ccatggtggt gatggtttcc cgagagctgg ccacggtggc tacacagaac
     2641 cggccgggtt ggcgctggaa gcgagggtta ttgatgccgg cgatggcgaa ggcaggttcc
     2701 cagtcggcca tgccatcgaa gacgtaaaga taaacggtct gcgcttccat gcgcgtctca
     2761 ggccttccgc ggagtgaatc ctggatttgc gtcgattgta cttcgccccg gagcgctgcc
     2821 cgtagttctt tccgtgttat tccgcgcgca gcgcctgcat tgggttaata gcggcagcgc
     2881 ggcgggccgg caggagcgag gcagcgagcg cgagcatcag gaccacaacc gcggcgagcg
     2941 tgaggacgag cggatcgagg gtgctgacgc cgaagacgaa ggtcttcagc aggcgcgaag
     3001 ccaacaatgc gccgaccagg ccgatgacgc atcccgcgcc tgccagcttg agggcggaga
     3061 acaggatgag gctgacgatc ccggagcgct gcgaacccag cgccatgcgg atggccattt
     3121 cctgagcgcg catggccacg gtgaaggcga ttacgctgta aatcccgaga atggcgagca
     3181 gaaccgccgc agcagcaaag gcggagatca gcacggtatt aaaacggcgc ggcgcttccg
     3241 agtcgctgat ggcatgctcc atggtttgta actgtgtgag tggcaattgc ggatcgagcg
     3301 agcggacggc ggcgcgcagc tgattttcga tcatctccgg cggcagcgag gtgcggagga
     3361 cgatgtagcc accgttgccg ttgatatccg tttcgggatg gccgagcgag ccgaagaagg
     3421 tctccgcctg gtcgttctcg tagtagtact gctggcgcgc atccgcgtcg ggagagcctt
     3481 ctttcatgct ggcgacttcg ccgacgacag tggcccaggg ggtctgcatc tcggttgtac
     3541 cgatgcgcat gcgttttccg atcggatcct ggccaggcca ggattgacgc gcgaattcct
     3601 cgttaactac ggcaacgagc tgcgctttgg ggttgttgtc ggcatcggtg agcaggcggc
     3661 cgcgaagcag gcgaatgccc atagcatgga agagatcgcc ctggatctga atgggggtgg
     3721 cgagatcgat cgcgccggct tttgcaggaa catagccttc ggccacgaag gctgaattgc
     3781 cctgggttcc attggcgggc aggaacgaag taaggcccgc gctggtgacg ccggggattc
     3841 cctcgacgcg acgcaggagt tcctggttga actgatcgac ggcaggctgg gttgtgtact
     3901 gcttgcgcgg gaggctgtag gcggcaacga cggtgtggtc gggacgatag ccaaggtcgg
     3961 cttcgcgcat cttctcgaaa ctgcgcagca gcaaaccgga tgcagcgagg agaaccagcg
     4021 cgaccgcaat ctcggcaatg accagaccgg agcgcagacg agcatgccca gcgcccgagc
     4081 tgccggtgcg gccgccttcc ttcagggttt cgttcacact ggtgcgaatg gcagcgaagg
     4141 cgggcgcgac gccgcacagc gcgccggtga cgatggcgag agcgagggcg aaggtcgcaa
     4201 ccatccagtc aatgccgatg ctgccgatga gagggagagt ttcgggcagc aggctggtgc
     4261 cgagtttgag cgcgatggcg gcgaggatga ggccgatgat gccgccgccg acgctgagga
     4321 cgagactctc aagcagagcc tggcggagca gcgtgcggcc gctcgaaccg agggcaagcc
     4381 gaaccgcgat ttcgcggcgg cggcggatgg cgcggaccag aagcaatccg gccaggttcg
     4441 cgcatgcgat gagcagcaca accgccacag caagaaacaa cgtgcgaatg agcggccgag
     4501 cctgggcgat ggtgttctca tgcaggccgc gcaccagcgg gctgatgtgc agactcgcca
     4561 taaaggcagg atagttgcgc atggttgcct gagccacgcg gttggcgtct tcacgggcct
     4621 gttcgggcgt gacgccgtct ttgagacggc cgaccatgct gtaactccag ttggcctgag
     4681 cgccgccggt cagctcatcg gcgcgcaggc tgaggggaat ccacagctcg ctgcgattga
     4741 gatggccggg aaccagggga aattcgaagt tgcgcggcat gatgccgatg accacgtacg
     4801 gcttgcggtc gagcaggatc ttctgaccga ggatgctcga ctcgccatga aagcggctct
     4861 gccaggtcgc atagctgagg acggcgacct gctgatgctg cgcgtcctca tcggcggtgt
     4921 aggtgcggcc gagcatcggc tggacgccga gggcggcgaa gaccccaggg ctcatgcgcg
     4981 cggcgttgat ctgcgcaggg tcgccgatgc cggaaagctc gtagccgggc tgcgtgtagc
     5041 cgcccagcgc tgtgaagctg tgggtgtcgc gcgtgtagtt gacgatgtcc ggcccggtga
     5101 cgccggcttc gttgttcccg cccacgtcgg cgccctggat ggtgtcggtg aggaccacga
     5161 gctgctgcgg gtgcgggaag ggaagcgggc gcagaagcac cccttcgacg atggagaaga
     5221 tcgcagtggt ggcgccgatg ccgagagcca gcatgagaac ggcggtgatc gagaagcccg
     5281 acgatttgga gagatgacgg atggcgatct tcaggtcacg aagcacgaaa
//
LOCUS       DASZYN010000073         2802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_67779, whole genome shotgun sequence.
ACCESSION   DASZYN010000073 DASZYN010000000
VERSION     DASZYN010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67779"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VHE33_01970"
     CDS             <1..567
                     /locus_tag="VHE33_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96243.1"
                     /translation="LYDEVADGSAIPIVLTDLYPNALLARRAGSGVSVWHEPVDARHV
                     PAELRGFRTIFSAFHHFDPEAARAMLADAFERREGIAVIEGARRSAWTMVAVTAVPFL
                     AFRAALTAGPFRWDRVLWSCVIPVVPAVLWVDGILSCLRSYSLEDLRELTAGLSDADY
                     EWQIGDAPGRVPVRYAIGSRKGGTYGST"
     gene            complement(564..1298)
                     /locus_tag="VHE33_01975"
     CDS             complement(564..1298)
                     /locus_tag="VHE33_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96244.1"
                     /translation="MNTIEEPLTFRPGSRAQDWASLSRIGGIAAFILILYSLAMIGQL
                     LVLGGQPATVAEAFSLLQHHRIVALLRLDLPTVFVLPLYYVLFLGLAAALYRSHPAQV
                     ILATSLSFVGITLVLATPTALSLIPLSDKYAAATSDAARNQLLAAGQALMAADIWHAT
                     GAILGGILAQCGAVMICVVMLRSPVFTRTTAWLGIVMHSLDLAHIVFALLWPTAGFAL
                     MAAAGPLYPIWFFLIGRRLLRLSSAG"
     gene            1376..1921
                     /locus_tag="VHE33_01980"
     CDS             1376..1921
                     /locus_tag="VHE33_01980"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVT96245.1"
                     /translation="MTLITCEGAVTLASGYTSRTSIPGDGVMRVGCWIASVLACAFAL
                     PAAAQSSAADQVWAREKVYWQYVQAYDVEGYRSIWHADFLGWPFFSPGPVRKDHIADW
                     IATYRKSGQRLESYELEALSIQVTGNLATTTYRIHQHWVDKSGADHPSVSRIIHTWLR
                     GPDGTWTIVSGMSSLPDSTGH"
     gene            2541..>2802
                     /locus_tag="VHE33_01985"
     CDS             2541..>2802
                     /locus_tag="VHE33_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96246.1"
                     /translation="MMKIICRIAAVAMLVASGIHMEAQEARGVADKAGAGTAGMTVVS
                     PESEGFSSERLENLHRLIQGEIDAKELAGAVTILARHGKVVDY"
BASE COUNT          533 a          719 c         1027 g          523 t
ORIGIN      
        1 ctctatgacg aggtggccga tggcagcgcg atccccatcg tgctgacgga tctctatccg
       61 aatgcgctgc tcgcgcggcg tgcggggagc ggcgtgagcg tgtggcatga gccggtcgat
      121 gcgcggcatg ttccggcgga gctgcgcggg ttccgcacga ttttttccgc gttccatcac
      181 ttcgatccgg aggcggctcg ggcgatgctg gcggacgcat tcgagcgacg tgagggaatc
      241 gcggtaatcg agggcgctcg gcggagtgcg tggacgatgg ttgcggtgac ggcggtgccg
      301 tttctcgcgt ttcgcgccgc tctgacggcg gggccgttcc ggtgggatcg ggtgctgtgg
      361 agttgcgtga tcccggtcgt gccggcggtg ctgtgggtgg atgggatcct gtcgtgtttg
      421 cggagttact cgctggagga cctgcgcgag ctgaccgcgg ggctatcgga tgcggactac
      481 gaatggcaga tcggggatgc gccggggcga gtgccggtgc ggtacgcgat cgggagccgg
      541 aagggtggga cgtacgggtc cacttaaccc gcggaggaca ggcggaggag gcgcctgccg
      601 atgaggaaga accagatcgg gtagagcggg cctgcggcgg ccatgagggc gaatccggcg
      661 gtgggccaca gcagcgcgaa gacgatgtgg gccaggtcga ggctgtgcat gacgatgccg
      721 agccacgcgg tggtccgggt aaagacggga ctgcgcagca tgacgacgca gatcatcaca
      781 gcgccgcact gggcgaggat gcctccgagg atggcgccgg tggcgtgcca gatatcggcg
      841 gccatgagag cttgtcctgc cgccagcagt tggttgcgcg ccgcatcgga ggtggcggcg
      901 gcgtatttgt cgctcagcgg gatcagggag agggctgtgg gcgtggcgag tacgagggtg
      961 attcccacga aggagagaga tgtggcgagg atgacttgcg cggggtggct gcggtagagg
     1021 gcggcggcca ggccgagaaa caggacgtag tacagaggca ggacaaagac ggtgggcaga
     1081 tcgaggcgga ggagggcgac gatccggtgg tgctggagca gggagaatgc ttcggccacg
     1141 gtggcgggct gtccgccaag gacgaggagc tgaccgatca tcgcgagtga gtagaggatc
     1201 aggatgaagg cggcgattcc gccgatgcgg gacaggctgg cccaatcctg agccctggaa
     1261 ccagggcgaa acgtaagggg ctcttctatt gtgttcacat tgtgtcttcc ctgctccgga
     1321 attcgggcaa atccggtttg ggcgcgatgg tagcgcagca cagagactgc gatccgtgac
     1381 tctcatcact tgcgagggcg cagtcaccct ggcctccggg tatacttcgc gcacatcgat
     1441 tcctggagac ggcgtgatgc gagttggctg ctggattgca tctgttttgg cgtgtgcttt
     1501 cgcgctgccg gcggcggcgc agtcgagcgc ggccgatcag gtgtgggccc gggagaaggt
     1561 ctactggcag tatgtgcagg cgtacgacgt tgagggctat cgatcgatat ggcacgccga
     1621 ttttctgggc tggcctttct ttagtcctgg accggtaagg aaggatcaca tcgccgactg
     1681 gatcgccacc tacaggaaga gcgggcagag gctggagtcg tatgagctgg aggcgctgtc
     1741 gattcaagtg acggggaatc tggcgacgac aacgtatcgg atccaccagc actgggtgga
     1801 caagagcgga gcggatcacc catcggtgtc gcggatcatt cacacgtggc tgcgcggtcc
     1861 ggatggcacg tggacgatcg tgtcggggat gtcgtcgctg ccggattcga ccggacactg
     1921 agcgcgaagc ggctcggctc cgcggcggtt cgctcgaaac ctatggacgg agaagagttt
     1981 cagcgggcga gagcttgcgg cgggcgcctg tggattcctg tcaatcccca gccctcgcgt
     2041 tacgaacgtt ttctctttca ttccacgagg cttatacgcc aaacaacccg agaaaggttg
     2101 gcacgatttc taaccggaac tgctattctg ataataggga gacagaacga aaaagcccgc
     2161 cctcatcgag gcgggctttg tgtttctgtc agcatccccg atccggctca ggcccgatcc
     2221 aacgagattc ggtctttgaa aacctggcgt ccaggacggt gctgattcat ggccgctttg
     2281 tgttacaaac gcggccgcgt tgttacaaca cggtcgcctg tgcaacacca acgatcccct
     2341 ccgaagtgcc ttttttcata aagatagtcg cttgtcaagc tggtaaacaa acgcaaacca
     2401 caagaaacca attacatgca gaacggccct caggaaaaag agaatttctt ctaaccggcg
     2461 gatcgcggga atcagtcgac tgatccgctg cccctgcttt ggtataaagg tgccgcaaac
     2521 tcgaatcgag gacgtggcgc gtgatgaaaa taatttgccg gattgcggcg gtggcgatgc
     2581 tggtggcttc cgggattcac atggaagcgc aagaagcccg gggcgtagcg gacaaagctg
     2641 gggccgggac agcggggatg acggtggtga gtccggagag cgaggggttt tcgtcggagc
     2701 ggctggaaaa cctgcatcgg ctgatccagg gggagatcga cgcgaaggaa ctggccgggg
     2761 cggtgacgat tctggcgcgg catgggaagg tcgtcgatta cc
//
LOCUS       DASZYN010000074         7692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1224146, whole genome shotgun sequence.
ACCESSION   DASZYN010000074 DASZYN010000000
VERSION     DASZYN010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7692
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224146"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            317..1369
                     /gene="pstS"
                     /locus_tag="VHE33_01990"
     CDS             317..1369
                     /gene="pstS"
                     /locus_tag="VHE33_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HVT96247.1"
                     /translation="MSKRAWTAAIAVAALGGLSACNSGPNNVNIAGAGSTFVYPVMTR
                     WIQDFQQSHQNVHINYQSIGSGGGIQQVKAKTVDFGASDAPLSDQQISAMFPLIQIPE
                     SAGPVCITYNLPGLTQPLHLSPESVAGIFLGTIKTWTDAQLAKDNPGVKLPSVPVIVS
                     HRSEGSGTTSIFTTYLTAVSPDWKTKVGAGPAVSWPVGIGGKGSEGVTGNIRNSPGAI
                     GYVELTYAEQNHLPTAAIKNAAGQFIQPTAAGTTSAIAAYSAQLTQDPRTPIVNPPAN
                     AKDAYPIAGLTFLIIPKDGPDKAKRTALKQFIQYVISDGQATAGTLNYAPLPDGVKQY
                     DQQQLQQLTAEGQPIS"
     gene            complement(1424..2239)
                     /locus_tag="VHE33_01995"
     CDS             complement(1424..2239)
                     /locus_tag="VHE33_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96248.1"
                     /translation="MHRISLTLVALSLALPAAFAQQSSSSLISPSYLSNDSAAGNGSG
                     VSRSTPASQTRAFPGIDVGAKIGLLGPGFEVAVPVAPHFNVRGGANFFSYDYSGATNG
                     VNYTATLRMRSVGASLDWYPWAHGFRISPGALLYNGNRVLGNAGIPGGDDFTLNGTSY
                     VSDPADPVHGTGSLVFRKAAPALTVGWGNRLSGEGRHVSFPFEAGFAYVGDPTVDLTF
                     TGSVCDAGMQGCQSIANDPSAQANVAAERQKFQKDANYARFYPILSQGFAVRF"
     gene            complement(2413..3000)
                     /locus_tag="VHE33_02000"
     CDS             complement(2413..3000)
                     /locus_tag="VHE33_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96249.1"
                     /translation="MSSAANRSEEDPSARGTAALIVTNVEGIETRAAELAARLNITVE
                     VVASRSAALRLLARRSYVIVVVDQILADSESDGCELIWKNSGLAIPLQINFALVGSAR
                     LEREMRTALARREREKQLALQAAAAAVDDELKNAITAFLLESRLALAERNIPPPVESR
                     LQTLAGMADHLRDWLLPNRGTEYHERAIDRTPEVT"
     gene            complement(2997..4400)
                     /locus_tag="VHE33_02005"
     CDS             complement(2997..4400)
                     /locus_tag="VHE33_02005"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HVT96250.1"
                     /translation="MIAPFQPETAPLHILIADDDASIRRACSEIAANAGMVPHETNLS
                     ARSCDLLLSKAIDIFLVDETVATGAGSELLEKIRDMRPEVDVVWMTAASTIELALEAL
                     RYGASDYLIKPFSAEELSGVLKRLAERRTLDAASRGLREKLRTGKGAGNLLGQSPEME
                     KLFRIIARVAQTIHPVLILGESGTGKELVARAIHANGAGAGKPFVPVDCGSLAPTLIE
                     SELFGHVKGAFTGAHRPHDGLLASAGSGTVFLDEIAELPLDLQAKLLRALQEKEARPL
                     GGNHAVPIRARILAATHQDLPTMVEQGKFRRDLYYRLNVVKLTLPPLRDRKEDIPLLA
                     AHFLERNSVEMRRAFRLSAETLTFLMDYSWPGNVRELANAIDGACSMTSGEVIEIGEM
                     TTSIRDAWMHAKVSRRPPAEIALPETEVETLANMEKRAILTTLEQVHGDKLLAARLLD
                     IGKTTLYRKLKEYGEMP"
     gene            complement(4405..5400)
                     /locus_tag="VHE33_02010"
     CDS             complement(4405..5400)
                     /locus_tag="VHE33_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVT96251.1"
                     /translation="MQNATPAPALKTVNQILSSPTLPPALLPRLGSAPTLREEGGEAL
                     ALSGIAHDARNLVTALGLCAELISAPGVLAPKHGHFGAEIRSLAESSAQLVRRLTELT
                     RKTAEVQAQTDPEATVTDLSESVQKMRSLLTAIAGPVMEVQFACLPCAGHLRLSEESL
                     SRILVNLVRNAADAMPTGGRIRITTQRGNGASFLWAVDEGTEEQTKEYLWDDLPHAAK
                     TRSGTVVITVEDNGPGIPTEFLERVFDAGFTTRQQGKSWPESLHRGLGLSIVRELVQA
                     AGGTVRALSSPTNGARLEIELPLTNVTSNLLSERRQDAQTPDNDATMNQSFRSTD"
     gene            5715..6452
                     /gene="rph"
                     /locus_tag="VHE33_02015"
     CDS             5715..6452
                     /gene="rph"
                     /locus_tag="VHE33_02015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HVT96252.1"
                     /translation="MPSSFFRTGDRTPAQMRTVRLTPGYTQVAEGSVLIECGHTRVLC
                     NATVEQGVPAWLRNSGRGWVTAEYGMLPRATLTRTPRESERGKVGGRTHEIQRLIGRS
                     LRAVVDMKALGERTVILDCDVLQADGGTRTAAITGACAALAIAFNRMLKTEALKASPL
                     RQLVAATSVGIVENTVLLDLAYEEDSQAEVDMNLVMTEDGNFVEVQATAERHAFPRAR
                     LLEMMDYAEAGIRQLHEMQRAAIDSAG"
     gene            complement(6753..7304)
                     /locus_tag="VHE33_02020"
     CDS             complement(6753..7304)
                     /locus_tag="VHE33_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96253.1"
                     /translation="MECRHIFSNGKRCRCRALDTHVFCLHHAPQPRPRTLRPRNANLG
                     TWRQIQIHLATLSREEIPAAILTLLSSLLEDGPRSPSDRTAGALLRALVRTCGKVPFA
                     LPTDPAPERDWAFDLSQSMDRVCDIIRRNKQFDALESLGLLPSLQTAPSSHPNLSTSS
                     PLPNSTRMNNHPAPLVEKRGLSR"
BASE COUNT         1432 a         2351 c         2341 g         1568 t
ORIGIN      
        1 tcccacccct taatttcacc gctcttcggg gcgatcacat gggtcatcgc accagcttca
       61 tccagcgctt tgcgcggttt gatcaactct gcctgctcaa aaccatcggt cgccaggatg
      121 gctatcattt ttcctgccag gttgcctgtc atcttgtacc ctccttctca tctctttgca
      181 ctcttctcgt taagaaggaa cagcgccatt cggcgttgcc agttttcccc attcttctgg
      241 ttgtaacaat gggttgcaca aaatcgtgta gagttccgtg ggtgaggaat tagcaacacc
      301 cgtggaggag attttcatgt cgaagcgagc gtggactgcg gcgatcgcag tcgcggcttt
      361 gggcggtctg agcgcctgca attcgggtcc caacaacgtc aacattgccg gcgcgggcag
      421 cacgtttgtc tacccggtca tgacgcggtg gattcaggat tttcagcaaa gccaccagaa
      481 cgtccatatc aactatcagt ccatcggctc aggcggtggt atccagcagg tcaaagcaaa
      541 aaccgtcgac ttcggcgcga gcgatgcgcc tctcagcgat cagcaaatct ccgcgatgtt
      601 tccgctcatc cagatcccgg agagcgccgg cccggtctgc atcacctaca atttgcccgg
      661 actcacacag ccgctccatc tgtcgcctga atccgtggcg ggcatctttc tcggaaccat
      721 caaaacctgg acggacgcgc agctcgcgaa agacaatccg ggtgtgaagc ttccttcggt
      781 tccggtcatc gtgtcccatc gctcagaagg cagcggtacc accagcatct tcaccaccta
      841 tctcaccgcc gttagccctg actggaagac caaagtcggc gctggccccg ccgtgagctg
      901 gcccgtgggc atcggtggca aaggcagtga aggcgtgacc gggaacatcc gcaattcacc
      961 cggcgcgatt gggtatgtgg aactgaccta cgccgagcag aatcatctgc cgacggcagc
     1021 cattaagaat gccgccggac aattcattca gccgactgcg gccggtacca cttccgcgat
     1081 cgcagcctac agtgcgcaac tcacccagga cccgcgcaca ccgatcgtca atcctccggc
     1141 gaatgcgaag gatgcttacc ccatcgccgg gcttaccttc ctgatcattc cgaaagatgg
     1201 cccggacaag gcgaagcgca ccgctctgaa gcaattcatc cagtacgtga tttccgacgg
     1261 acaggccact gcggggacgc tgaactacgc gccactgccc gatggagtaa agcagtacga
     1321 tcagcagcaa ctccagcaac tgacggctga aggccagccg atcagttagc cgcgagttgg
     1381 ttcaggggcg cgtggaggtt accctggcgc ccctgcgcgt tccctagaat cggaccgcga
     1441 acccctgcga aagaatcggg tagaagcggg cgtagttggc gtccttctgg aacttctgcc
     1501 gctcagccgc cacattggcc tgcgcactgg gatcgttggc aatcgactgg cacccctgca
     1561 tcccggcatc gcaaacgctt cccgtaaagg taaggtctac tgtcggatcg cccacatagg
     1621 caaagcccgc ttcaaacggg aagctgacgt gacggccttc gcccgataac cggtttcccc
     1681 atccgaccgt gagcgccggc gccgccttgc ggaagacaag gctgcctgtg ccgtgcaccg
     1741 gatcggccgg gtcactgacg tagctggtgc cgttcagcgt gaagtcgtcg ccgcctggaa
     1801 ttcccgcatt tcccaggacc cggttgccat tgtagagcag cgcaccagga ctgattcgaa
     1861 agccgtgcgc ccatggatac cagtcgaggc tggctccgac cgaccgcatc cgcagggttg
     1921 ccgtgtagtt cacgccgttc gtcgcaccgg aatagtcgta gctaaagaag ttcgcgccac
     1981 cgcggacgtt gaaatggggc gcgacgggta cggcgacttc gaagcccggc ccaagcaggc
     2041 cgattttcgc cccaacgtca attccgggga aggcgcgcgt ctggctcgcc ggcgtggaac
     2101 ggctcacgcc ggacccgttt ccggccgccg agtcgttcga aaggtaagac gggctgatca
     2161 gagacgaact gctttgttga gcaaacgccg ctggcaatgc cagcgacaga gcaaccaggg
     2221 tcagagaaat gcgatgcata agggggaggg ctcctcaaga gtgtggctgt gatgcacgcg
     2281 ccatccaatc aagctgtctt aactgcttga ttccacacct ctttcctggc ggaggtggga
     2341 aaatcaggtg gacaacgcca cacgctttat gcctcgttgg tacctgactt ccgcggcgaa
     2401 tcagtaacaa ggctaggtca cttcgggggt gcgatcaatg gcacgctcgt ggtactccgt
     2461 gccacggttc ggtagaagcc aatcccgaag atggtccgcc atcccggcca gggtctgcag
     2521 ccggctctcc actggcggtg gaatattccg ctccgccagc gccagacgcg actcgagcag
     2581 aaatgccgta atcgcattct tcagctcgtc gtcgactgcc gccgcagcag cctggagcgc
     2641 aagctgtttc tcccgctctc gccgcgcgag cgccgttcgc atttcgcgct ccaaacgcgc
     2701 actccccacc agtgcgaagt tgatctggag cggaatggcc agcccgctgt tcttccagat
     2761 cagttcgcat ccgtcggatt ctgagtcggc cagaatctga tcgacgacga caatgacgta
     2821 ggaccttcgt gccagcaggc gcagcgccgc ggagcggctg gcgacgacct caacggtgat
     2881 gttcagacgg gcagccagct ccgccgccct ggtttcgatt ccctcaacgt tcgtgacgat
     2941 cagcgccgcc gttcctctcg ccgagggatc ttcctcactt cggttcgcag cggagctcat
     3001 ggcatttctc cgtattcctt caacttgcgg tacagagtcg ttttcccaat gtcgagcaat
     3061 ctggcggcca gcagcttatc gccgtggacc tgttccaggg tggtcaggat tgctcgcttt
     3121 tccatgttcg ccagcgtttc gacctctgtt tcaggcaatg ctatttccgc tggcggtcgc
     3181 cggctcactt tcgcatgcat ccacgcatcg cggatgctgg tggtcatctc accgatttca
     3241 atgacttcgc ccgaggtcat gctgcatgcc ccgtcaatcg cgtttgcaag ctcccgcaca
     3301 ttgcccggcc acgagtagtc catcaggaaa gtcagtgtct ccgcactgag tcgaaaggct
     3361 cgccgcatct cgacgctgtt ccgttccaga aagtgcgccg ccagcagggg aatatcctct
     3421 ttccgatccc gtaacggcgg cagcgtcagc ttgacgacat tcagccggta gtaaagatcg
     3481 cgccggaact ttccctgctc caccatggtc ggcagatcct ggtgtgtcgc ggcaagaatc
     3541 ctggcccgga tggggacagc atgattgccg ccgagagggc gcgcttcctt ttcctgaaga
     3601 gcgcgcagca gtttcgcctg cagatcgagc ggcagttcgg caatttcgtc cagaaacacg
     3661 gtcccgctgc cggctgacgc caggagtcca tcgtgcgggc gatgggcccc ggtgaaagcc
     3721 cccttcacat gtccgaacag ctcgctctcg atcagcgtgg gagccaggga cccgcaatcc
     3781 accgggacga aaggtttgcc cgcccctgcg ccattcgcat ggatcgcacg cgcaaccagt
     3841 tctttaccgg tcccgctctc gcccagaatc agcacaggat ggatggtctg cgcgactcgt
     3901 gcgatgatcc ggaacagctt ctccatctcc ggactctgac cgagaagatt gcctgcgccc
     3961 ttacccgtcc ggagcttctc ccgcaaaccg cggctggccg catccagcgt gcgcctttcc
     4021 gcgagccgct tcaatacacc cgacaattcc tctgcggaga agggcttgat cagataatcg
     4081 ctggccccgt agcgcagggc ttcgagggcc agttcaatcg tcgaagcggc ggtcatccag
     4141 acgacatcga cttcggggcg catgtcgcga atcttctcca gcagctcgct gcctgcgccg
     4201 gtcgcgacgg tttcatccac caaaaagatg tcgattgctt tggacaacaa taggtcgcag
     4261 ctccgcgcgg agaggtttgt ctcgtgcggc accatgccgg cgttcgcagc aatttcgctg
     4321 cacgcccgtc gaatcgacgc gtcgtcatcg gcaatgagga tgtgaagtgg agcagtctca
     4381 ggctgaaaag gagcgatcat ttgactaatc cgtggatcga aagctttggt tcatcgtcgc
     4441 atcattgtcc ggcgtttgag catcctgacg ccgttccgaa agtaagttcg acgttacgtt
     4501 tgtaagtggc agctcgatct ccagacgtgc tccatttgtc ggagaactta gcgcgcgcac
     4561 cgtaccgccg gcagcctgca caagctcgcg cacgatgctc agccctaacc cgcgatgcag
     4621 cgactccggc catgacttac cctgttgacg agtcgtgaag cctgcgtcaa atacccgctc
     4681 cagaaactct gtcggaattc caggaccgtt atcttcgact gtgatcacta cggtgccgct
     4741 cctcgttttc gcagcgtgcg gaagatcgtc ccacagatac tctttcgtct gttcctcggt
     4801 cccttcgtca accgcccaca ggaagcttgc accgttgcct ctctgggtcg tgattcgaat
     4861 tcggcctcct gtcggcatcg catcggcggc attgcgcacc aggttcacca gaatccgcga
     4921 caaactttcc tcgctcagcc gcagatggcc cgcgcagggc aggcaggcaa actgcacctc
     4981 catgaccgga cccgcgattg ccgtcagcag actgcgcatc ttctgaacgg actccgacaa
     5041 atccgtcacg gtcgcctccg gatcggtctg cgcctgaacc tctgccgtct tgcgggttag
     5101 ttccgtcagc cgtcgaacca gctgtgcgga cgactcagcc agggagcgaa tctccgcacc
     5161 gaaatgccca tgcttcggag ccagcacgcc aggcgcgctg atcagttccg cgcacagtcc
     5221 gagcgcggtc acgagattgc gggcatcgtg tgcgattccc gagagtgcca gggcttcgcc
     5281 gccctcttct cgcagagttg gcgcgctgcc cagccttggc agcagtgcag gtggcaacgt
     5341 gggggacgag agaatctgat tgaccgtctt cagggccggt gccggcgttg cgttttgcat
     5401 gagtccttcc ttctcaagcc ctggagccgt tgcgctctgg atcgccgcgg ctccggcctg
     5461 gttcacgact gtcccgtgtt agtacacgtc agggaccaaa ccgctgcggt ctggcaaagc
     5521 ccggaatcat cagcacttgt gcggttcggg ccttatttcg ccaactcggt gtcgatgatc
     5581 gattggttcc atattggaac tggaccccgg ccgtccagca ctgcaacctc tgctccttct
     5641 tcgatttcgg tgctctgttc catttccgaa ccgaacgaag cggtttcccg gttcggaact
     5701 cgtacaatga gccgatgcct tcctcctttt ttcgtactgg cgaccgcacg cccgcgcaga
     5761 tgcgcacggt tcgcctcact cccggctaca cccaggtcgc cgaagggtcc gtcctgatcg
     5821 agtgcggcca cacccgggtt ctctgcaacg ccacggtgga gcagggcgtt ccggcatggc
     5881 tgcgtaattc cggtcgcggc tgggttacgg ccgaatacgg catgttgccg cgcgcgacgc
     5941 tcacccggac cccgcgtgaa tcggagcgcg gcaaggtcgg cggacgcacg cacgagatcc
     6001 agcggctgat cggccgctcg ctgcgcgccg tcgtcgacat gaaagcgctc ggcgagcgaa
     6061 cggtcatcct cgactgcgat gtccttcagg ccgacggcgg cactcgcacc gcggccatta
     6121 ccggagcgtg tgcggctctc gcgatcgcgt tcaatcggat gttgaagacg gaggcgctga
     6181 aggcttcgcc actgcggcag ctggtggctg cgaccagtgt cggaatcgtc gagaacacgg
     6241 ttctgctgga cctggcctat gaggaagact cgcaggccga ggtggacatg aatcttgtca
     6301 tgacggagga tggcaacttc gtcgaagtcc aggcgacggc ggagcggcac gcgtttccgc
     6361 gggcgcggct gctggagatg atggactacg ccgaggccgg catccggcag ctccatgaga
     6421 tgcaacgggc cgcgatcgac tccgcgggct gagcctggtc agtgttgccg cgaggccgga
     6481 gaggtgatcg atttgtcacc acgaacccga cgcccctaag acaagaatgc gggacgcctg
     6541 ctgattctgc tgatgttgcc cagggggatt tgtgttgaca acctgtgtgt ggccgcagcc
     6601 gtgtttacgg gcgagataag aggtaggcgg ctgggcgccg gcagggcgcg tgtgcgcggg
     6661 acacgcctgg tattctccgg atccggctca tctggcaaca ccctgcagtg gccgtgtccg
     6721 ggtttcggat acggcttgtg atatctcctt tgtcaccgcg aaagccccct tttttcgaca
     6781 agaggtgcgg gatggttgtt cattcgcgtg gagttgggca gaggggatga ggttgacaag
     6841 ttggggtggg acgatggcgc ggtttgcaga gaaggcaaca agcccagcga ctccagagcg
     6901 tcgaattgtt tgttgcgccg gatgatgtca cagacgcgat ccatggactg ggagagatcg
     6961 aacgcccagt cgcgctcggg ggctgggtcg gtggggaggg cgaaggggac cttgccgcag
     7021 gtgcggacga gtgcgcggag cagggcgccg gcggtgcggt cggaagggga gcgggggccg
     7081 tcttcgagaa gggagctgag cagggtgagg atggcggcag ggatctcttc gcgggagagg
     7141 gtggcgagat ggatctggat ctgacgccag gtgccgaggt tggcgttgcg gggccggagg
     7201 gtgcgcgggc gaggctgcgg cgcgtggtgg agacagaaga cgtgggtgtc gagggcgcgg
     7261 cagcggcagc gtttgccgtt ggaaaagatg tggcggcatt ccatggttgg gtcctcggga
     7321 gggttcggcg ctccggcgtt tggcgttcag tccttgcttt gttcaagggc aggttgttcg
     7381 aagtacggaa gaatcagacg tcctggcgat caaaagaaaa agcccgcgca aggcgggctt
     7441 ttcaatcttc gactctaagt tcaggatatc aggttgagat agaaatcgtg ccaaacttcc
     7501 tggcttgtaa gtggtgtgct atcaagccaa gttactttgg tgaagaggtt gagggcttga
     7561 caatttttcc gggggggcag caagggtctg cctctcgggg gcgtcgaatt cacggctggt
     7621 ttgtgcttgc ttctttgggg acccttcctg tcgcggcgct ccaaccttcg aaccacgcgg
     7681 tttcgcgcgc gg
//
LOCUS       DASZYN010000075         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_67816, whole genome shotgun sequence.
ACCESSION   DASZYN010000075 DASZYN010000000
VERSION     DASZYN010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67816"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="VHE33_02025"
     CDS             complement(<1..1206)
                     /locus_tag="VHE33_02025"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HVT96254.1"
                     /translation="MDNRSIANLLAETADLLEIGAGDPFRIRSYRRAAEAVEQTTIQL
                     STIAADAKKLQEIPGIGKGMAANIQAIESAGTLPLREELLLKYRPTMLELLRLPGMGP
                     KTVALLWDALQVGSVEDLEKAIAEGRLAGLPRMGEKLVEKLKKGIEDYKQRSGRFLID
                     DAEEAAEKLIAYLQRFDGITTILPAGSLRRGRDTVGDLDILATGPACAENCVDAAVEY
                     VANYPAANTMLAKGQNKVSFRLRSGLQVDVRLLPVGSYGAALQYFTGSKMHNVSVRQR
                     ALKRGYTLSEYALAKVEDGSFVAGATEEEIYAALGMDWIPPELRENNGEIEAAEEHRL
                     PVLIEENDIRGDVHMHTVETDGKNTIREMADAGVKRGYEYIAITDHSKNLAMTMGLDD
                     KRALEHIARI"
     gene            complement(1412..1654)
                     /locus_tag="VHE33_02030"
     CDS             complement(1412..1654)
                     /locus_tag="VHE33_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HVT96255.1"
                     /translation="MAQYKGKVKWFNNAKGYGFIGRDDGPDVFVHYSAIQVDGYKSLK
                     EGDDVEFDIVQGQKGPQADQVIRARETNPNAGADAA"
BASE COUNT          324 a          631 c          486 g          406 t
ORIGIN      
        1 aatccgcgca atgtgctcca gggcccgctt atcgtcgagc cccatcgtca tggcgaggtt
       61 cttcgagtga tcggtgatcg cgatgtactc gtagccgcgc ttcacgccgg catcggccat
      121 ctcgcgaatc gtgttcttcc cgtccgtttc gacggtgtgc atgtgcacat cgccgcggat
      181 gtcgttttcc tcaatgagca ccggcagacg atgctcctcc gccgcctcga tctcgccgtt
      241 attctcgcgc agctccgggg gaatccagtc catgccgagc gcggcgtaaa tctcctcctc
      301 cgtcgcgccg gcaacaaacg atccgtcctc aactttggcc agggcatatt cgctcagcgt
      361 gtatccacgc ttcagagccc gctgccgcac gctcacgttg tgcattttcg atccggtgaa
      421 gtattgcaaa gccgcgccat acgatcccac cggcagcagc cgcacgtcga cctgcagccc
      481 gctccgcagg cggaagctca ccttgttctg tcccttcgcc agcatggtgt tcgccgccgg
      541 ataattggcg acatactcca cggccgcatc gacgcaattc tccgcgcacg ccggtccggt
      601 cgccagaatg tccagatcgc ccaccgtgtc gcgcccgcgc cgcagcgatc cggcaggcag
      661 aatcgtcgtg atgccgtcga acctttgcaa atacgcgatc agcttctccg ccgcctcttc
      721 cgcgtcgtca atcaggaaac gcccgctgcg ctgcttgtaa tcctcaatgc ccttcttcag
      781 cttttcgacg agcttctcgc ccatccgggg cagcccggcc agccggccct cagcaatggc
      841 tttctccagg tcttcgaccg agcccacctg cagcgcgtcc cacagcagcg ccacggtctt
      901 cgggcccatg ccgggaagcc gcagcagctc cagcatcgtc ggccggtatt tcagcagcag
      961 ttcttcccgc aggggcagcg ttcccgcgct ttcaatggcc tggatattcg ctgccatccc
     1021 cttgccgatg ccggggatct cctgcagctt cttcgcatcg gccgcaatcg tgctgagttg
     1081 aatcgtggtc tgctcgacgg cctccgcggc tctgcgatac gaccggatac gaaacggatc
     1141 gcccgcacca atctccagca ggtcggccgt ctccgccagc aaattcgcaa tcgatcgatt
     1201 gtccatcgag gattgattac accacgtcgc gaaaacccaa accagagaca cacgcaggtc
     1261 tgcgctcgat acttttcagg gtaccggtgc agaccgcagc tcgccagcgc gcaattgaac
     1321 tgagacaggc ttcgagcgga agcgctcacc cgtctgcagc acacacgcag gtttcacatg
     1381 ttttgaagtc aggagtcggg aggtttggac gtcaggcggc gtcggcgccc gcgttcggat
     1441 tcgtctcgcg agctctgatc acctggtcgg cctgaggtcc tttttgaccc tggacgatgt
     1501 caaactccac atcgtcgccc tctttcaggc tcttgtatcc gtccacctga atggcgctgt
     1561 agtgaacgaa aacatcaggc ccgtcgtccc gcccaatgaa accataccct ttggcgttgt
     1621 tgaaccactt cactttgcct ttgtactgag ccacgtacgc gaaccttcct ctcaggcagt
     1681 gtggttgggg ttgccaacct gctctgatag agacgccggt gcgcaaagtt tggttttctg
     1741 cgattcgttc gcagattcca cagggcctat ttcttcttcc gctgcttccg gctccagcgg
     1801 gcgtaataca acactgaaat cagcgtcttg ccgtccagaa tcttccc
//
LOCUS       DASZYN010000076         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1031417, whole genome shotgun sequence.
ACCESSION   DASZYN010000076 DASZYN010000000
VERSION     DASZYN010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031417"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VHE33_02035"
     CDS             <1..609
                     /locus_tag="VHE33_02035"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVT96256.1"
                     /translation="SDVTKPAQATIYFPILSGIPDRTSEATIVAWTAGDPLAMSMPIQ
                     QQISALEPELPVYNALTMDQILGKTTASQGFAANLVLAFAGLALLLAAVGLYGVLSYL
                     VTQRVSEIGIRVALGAQRVQVLRLVLFDGLRPAFLGLMAGLSASAGVTQLIRSMLYGT
                     EPYDPAVLGAVIFVLLLIASIACMLPAWRASRVDPMQALRAE"
     gene            779..>1708
                     /locus_tag="VHE33_02040"
     CDS             779..>1708
                     /locus_tag="VHE33_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT96257.1"
                     /translation="MRLLDAIRARWSAPRHRSARMDELEEELRDHVRHRADDLERSGM
                     SRAEAERQARIEFGGVERYREEAHAAEGWHWAETLLRDAGFGLRILKKSPAFTATALI
                     TLALGIGANAALFTLVRGILLRPLPVRAASRLVVIWDSNPGVGLSRVGPTGQDYLDWR
                     EQKLHAFEDLFLFEHGTGTVTGNGEPEQLAGLRVTTNFGNFFGIAPTAGRTFLPEEAN
                     ARRTLAILGHRYWERKYQGNPAVCGQSMTLNGEDYTIIGVLPASFDELFPVDVVVPFD
                     DAWLHRADSDLGVFGRLRPHVSVRQAAAEMSAAM"
BASE COUNT          294 a          489 c          586 g          339 t
ORIGIN      
        1 tccgatgtga ccaaaccggc gcaggcgacg atctatttcc caatcctatc cggcatcccc
       61 gaccgcacca gcgaggcgac gattgtggcc tggacggcgg gcgatccgct ggcgatgtcg
      121 atgccgatcc agcaacaaat ctcggcgctg gagccggagt tgccggttta taacgcgctg
      181 acgatggatc agattctcgg caagacgacg gcgagccagg gattcgcggc gaatctggtg
      241 ctggcctttg ccggtttggc gcttctgctg gccgctgtcg ggctttacgg agtgctctcc
      301 tacctggtca cgcagagggt gtcggagatc ggtatccgcg tcgccctcgg cgcacaacgc
      361 gtgcaggtcc tgcgtcttgt cctgttcgat gggctgcggc cggcgttcct cgggctgatg
      421 gctggtttgt cagccagcgc aggcgtgaca caactgatcc ggtcgatgct ctacggaacc
      481 gaaccgtatg acccggctgt tcttggcgct gtcatcttcg tcctgctctt gatcgcatcg
      541 attgcgtgca tgctgcccgc ctggcgcgcc tcgcgcgtcg acccgatgca ggccctgcga
      601 gcggagtgaa gccggggaag acgagttcta ttcaatggtg ctcaaggcac cacggtggcg
      661 gcggattccg gttgaactcg tccgcacgag cggccggcgc tcgctttcct tgacaactta
      721 ggagagtggc ctgtatcgtc ctaagttatt taggagagat gcgaccgcca ggcgaattat
      781 gagactcctc gatgcaatcc gggcacgctg gtcggcccct cggcaccgtt cggcaaggat
      841 ggacgagctt gaggaagagc tgcgggacca cgttcggcat cgcgcggacg atctggaacg
      901 cagcggcatg tcacgggcgg aggcggagcg tcaggcccgc atcgagtttg gcggagtcga
      961 acgctaccgg gaagaagcgc atgccgccga gggatggcac tgggcggaaa cgcttttgcg
     1021 ggatgcaggg tttgggttac gaattctgaa gaagtcgccg gcgttcactg ccaccgcact
     1081 catcacgctg gcgctgggca tcggggcgaa tgcggcgctg ttcacactgg tgcgcgggat
     1141 tcttctgcga cccttgccgg tgcgcgcggc cagccgcctg gtcgtgatct gggacagcaa
     1201 tccaggagtg gggctgagcc gggttgggcc caccggacag gattatctgg actggagaga
     1261 acagaagctc cacgcgtttg aggatctgtt tctcttcgag catggcaccg gcacagtgac
     1321 gggaaatggg gagccggaac agctggcggg actgcgcgtg accacgaact tcgggaattt
     1381 tttcggcatc gcgccaactg cgggaagaac gtttctgccc gaagaggcga atgcgcggcg
     1441 cactctggcg atcctgggtc atcgctactg ggaacggaaa taccagggca atccggcggt
     1501 atgcgggcag agcatgacgc tcaacggaga ggactacacc atcatcggtg tgttgcctgc
     1561 ttcgtttgac gagctgtttc cggtggatgt cgtggttccc ttcgacgatg cctggctgca
     1621 ccgggccgat tccgatctgg gtgtcttcgg gcggctgcgg ccgcatgtat ccgtgcgcca
     1681 ggcggcggcc gagatgagcg cagcgatg
//
LOCUS       DASZYN010000077         6828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1095843, whole genome shotgun sequence.
ACCESSION   DASZYN010000077 DASZYN010000000
VERSION     DASZYN010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6828
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095843"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VHE33_02045"
     CDS             <1..355
                     /locus_tag="VHE33_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT96258.1"
                     /translation="IKFVGVPVTDQDRALKFWTEAVGLKVTTDQPFNEKQRWIELKVP
                     GAETGISLFTPDGHESRIGQFQSISFWSDDVFATADAMKKKGVVFAQEPKKEHWGTSA
                     VFKDPDGNMYVLSSR"
     gene            complement(394..1194)
                     /locus_tag="VHE33_02050"
     CDS             complement(394..1194)
                     /locus_tag="VHE33_02050"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT96259.1"
                     /translation="MPTNDQDSTLSLVQPSTAPIRVLLLDDHPDNLLLRSTILRKYGY
                     VTETASTVEEAERLLDEIDIAVLDYHLGAGKFGTDVAARLRELHPQVPIIILSATLER
                     RFGGVEDMHLLKGYSSIDDLVTALRSFEAKRRGKPVVVDARDFFYSRINMAMGDDVVL
                     EILDGDGTWQYVNETCARVLEKPRDWFPGRNMFEEMPSLAADWADILSTVAETRETYI
                     DRTFRGVLNLPSKGDRWVWNVLAFPITLHTRETGVVLTARALERKSLV"
     gene            complement(1270..2274)
                     /gene="lpxD"
                     /locus_tag="VHE33_02055"
     CDS             complement(1270..2274)
                     /gene="lpxD"
                     /locus_tag="VHE33_02055"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HVT96260.1"
                     /translation="MQSGELARVLEAELHGAADVEITGVAGIEEAEPGQITFVANPKY
                     AGLAKTTRASAILVTPDFPEVPAVTLRLKNPYLAFARAVEMFYQAPSWPEGIHPTAVI
                     HPTAQLGKRARIGAYAVVAENVVIGDDAILLPHVVLYPGVRIGDRFFAHAHAVVRENC
                     QLGNDVVLQNGVVVGSDGFGFAKDEHGRWHKIPQSGPTILGDRVEIQANACIDRASVG
                     ATHIGDGVKVDNLVQVGHGSSVDENTLLCAQVGLAGSTHVGKNVILAGQVGVAGHCKI
                     GDNVIAIAQAGLHGDIPANSMLAGSPAFEHKQWLRATALFARLPELLRQFQRKPKREE
                     "
     gene            2465..2779
                     /locus_tag="VHE33_02060"
     CDS             2465..2779
                     /locus_tag="VHE33_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96261.1"
                     /translation="MFAWGLRYKLSLYDPPHSITRHMPAAKLLTGNERRAVAAVDLRP
                     ASGLPVLAFLGALVFSVLFFRRPRVIPAFGSSALFPARRPPVPSWAPDGAKFIRPPPR
                     IL"
     gene            2909..4198
                     /locus_tag="VHE33_02065"
     CDS             2909..4198
                     /locus_tag="VHE33_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVT96262.1"
                     /translation="MRTRLWIVISAVIGVAAICVLAWRGHHPTNTVEAAPGPPAAQVA
                     LVQRGDIAQVLTLAGQFQPYQVVDVHPKVSGFIRHIYVDIGDRVRQGQTLAVLEVPEL
                     KAQLQGTISEVARSSDEIVRAQHEVRRAESTHAALHTDYQRLLDTSKAQPGLVAQQEL
                     DDAQAKDLASEADVDAAKAALAAAGKGMEVSRADNARVSAIEDYTNVIAPLDGVIVWR
                     YADTGALIQAGISSNNQDLPIVKLAEANVMRLRLPVPETYVRYVHIGDPVKVRVDALN
                     RSFTGKVVRFTRELNFETRTMETEVDVDNPGLAIDSGMYANALMRLDHADQVLTVPVG
                     AVILHGDQEQVYVLDSGNHVWIRNVHVGIRGSQLAEIQSGLKKGDRVIVAAQAKYTSG
                     ETVTPIVEKTAASETNPQSGGMIDMNPEAASDNGGQQ"
     gene            4199..>6828
                     /locus_tag="VHE33_02070"
     CDS             4199..>6828
                     /locus_tag="VHE33_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVT96263.1"
                     /translation="MPKFALKYPFFILMLCLMITIVGVVNLFSMPVDLFPDIDMPVVV
                     VATFYSGMPPQQIEADITNTFERFFTLAANVDHSESRSLTGVSLIKIYFKPGTDPNAA
                     LSNVSNLAMADLRRLPPGTLPPVVLGLTASTQPVCLVTLEGKGLNETQLKDLAQFQVR
                     NQISNVPGASVPQPYGGTYRQIQVYVDPLKMEARNLSLSDVVHAVDSSNLILPAGDVR
                     IGNKDFNIYANSQFPTAEAMNQMPLKSVGNGSVLVADIGHAVDAGALQYNIVRINGQR
                     SVYVPVFKQGGNSNTLTIVDGMKQAIKHLVDIPESLKTAVVFDQAVFVKLALKQLVNE
                     AIIGLVLTGIMILLFLGSPRATVAVMLAVPLSALACMLMANMLGGTINTMILGGLALA
                     FSRLIDNGVVVLENIFRYMELGEPTRIAAEKGGTEVSLAVLAATVTTSIVFLPVVGLS
                     GVSKYLFTPLALGVVLSMFASYAFAMTVIPLFCALFLKGHGHGERDEAGELVDKHEEP
                     RKRRLGLEPMVAKFNEYFQRLVNRYEALVRKSLLRPGLTTAVILAGCAILLVATFPFM
                     GRAYFPRTDPGQFIINVRMPSGTRLEVSNEDIARVEKIIREEVEPSDLDMIVSNIGVY
                     PDLSSIFTSNASMDTAFVQTSLKEGHSIGSYTYMRRVQERLSREMPQLTTYFQAGGLI
                     DGVINQGLPAPIDLQVSSRNMEDAYQLAEKLAAKIRTFPNVSATYIPQDINYPGIQLN
                     IDREKASLIGLSAKDVVDNVITALTSDGMVAPTYWIDPHSGNNYMVTVQYANHWIQNM
                     SMQDFKSIPLRGTHPPGFSPVDVGRTASSFSEDMMSGSHRAGYTPLSSVADIRLINTP
                     TEVDHYQIRRVIDVYVSTKS"
BASE COUNT         1328 a         2084 c         2035 g         1381 t
ORIGIN      
        1 catcaaattc gtgggcgttc cggtcaccga ccaggatcgc gcattgaagt tctggaccga
       61 ggcggtagga ctgaaggtaa cgacggacca gccgttcaac gagaagcagc gctggatcga
      121 actgaaggtt ccaggcgcgg agacaggcat ttcacttttt acgcctgacg gccacgagag
      181 ccgcattggg cagtttcagt cgatttcatt ctggtcggat gacgtcttcg cgacagccga
      241 tgcgatgaag aaaaaaggtg ttgtcttcgc tcaggagccg aagaaggaac actggggcac
      301 gtcggccgtg ttcaaggatc cggacggaaa catgtacgtg ctgtcgagcc ggtgagaaga
      361 ggcgcagctt tcaggtcaat gtcgcagatc cgtctacacc agcgacttgc gttcgagcgc
      421 gcgagcggtc agcacgacgc ccgtttcccg ggtgtgcagc gtgatgggaa acgccagcac
      481 attccagacc cagcggtcgc ctttgctggg cagattaagg acgccgcgga aggtgcggtc
      541 gatgtacgtc tcgcgggttt cggctacggt gctcaggata tcggcccagt cggccgccag
      601 acttggcatc tcctcgaaca tgttgcgtcc aggaaaccag tcacggggct tttccagcac
      661 gcgggcgcac gtctcgttca cgtattgcca ggtgccatcg ccgtcgagaa tctccagcac
      721 cacatcatcg cccatcgcca tgttgatgcg ggaatagaag aaatcgcgcg cgtcgacaac
      781 gaccggtttg ccgcgccgct tggcctcaaa tgaccgcagt gccgtaacca gatcatcgat
      841 ggagctatac cctttgagca ggtgcatgtc ctcgacgccg ccaaaccggc gctcgagcgt
      901 ggccgataaa ataatgatcg gcacctgagg atgcagctcg cgcagacgcg cagccacatc
      961 cgtgccaaat tttcccgcgc ccaggtgata gtccagaacg gcgatgtcaa tctcatccag
     1021 cagccgctcc gcttcctcca ccgtgctggc cgtttcggtg acatatccgt atttgcgcag
     1081 gatggtcgag cgcagcagga gattgtcggg gtggtcgtcc agcagcagta ctcgaatcgg
     1141 cgcggtactg ggttgcacca gcgacagcgt ggagtcctgg tcgttcgtgg gcataagtat
     1201 tcttttgatg caatagacgg cgaggatgct gttttggccg ccgacaattt ggatgcgttt
     1261 cgtattactt tactcttcgc gctttggttt gcgctggaat tgtcgcagca attccggtaa
     1321 gcgggcaaag agcgccgtag cgcgcagcca ctgcttgtgt tcgaaggcgg gcgagccggc
     1381 gagcatagag ttggctggga tatcgccatg caggcccgcc tgggcaatgg cgatgacgtt
     1441 gtcgccgatc ttgcaatggc ctgcgacgcc cacctggccg gcgagaatga cgtttttgcc
     1501 cacgtgcgtc gatccggcaa ggccgacctg cgcacacaac agcgtgtttt cgtcgactga
     1561 cgaaccgtgc cccacctgca ccagattgtc gactttgacc ccatcgccga tgtgcgtggc
     1621 gccaacactg gcgcggtcaa tacaggcgtt ggcctgaatc tccacgcgat cgccaaggat
     1681 ggtgggtccc gactgcggga tcttatgcca tcttccgtgc tcgtccttgg caaagccgaa
     1741 gccgtcggag ccgaccacaa cgccgttctg gagaacgaca tcgttgccga gctggcagtt
     1801 ttcacgcacc acggcatgag catgcgcaaa gaagcggtcg ccgatgcgga ctcctggata
     1861 aagtacgaca tgaggcagga ggatggcgtc atccccgatg accacgttct ccgcgacaac
     1921 cgcgtaggct ccgatccgcg cgcgtttccc cagttgcgcg gttggatgga tcacggccgt
     1981 ggggtggatc ccctccggcc acgacggcgc ctgatagaac atctcaaccg cgcgggcaaa
     2041 ggccaggtag ggatttttca ggcgcaaggt gactgcaggc acctcgggga agtccggcgt
     2101 gacgagaatc gcgctggcgc gggtggtttt tgccaggccg gcgtactttg gattcgcgac
     2161 aaacgtgatc tgtccaggct cggcttcttc gatgccggcc acgcccgtaa tctcgacatc
     2221 ggccgcgcca tgcagttccg cttcgaggac gcgcgccagc tcgccagatt gcatgcggcg
     2281 attgtaaatc gcccgagcgg gctatatttc gataggttcg taccacgctc gcgccctgga
     2341 aaacgtcaaa tcagtgtgtc atcatcgtgt tagcacggtg acgatcccac agccctaccg
     2401 attgcgtaaa caagaaccag ctctgcgcgc gctcgccata ggcctggcat gtctggggat
     2461 gtgtgtgttt gcctggggac tgcggtacaa gctctccctg tacgatccgc cgcactccat
     2521 cacgcgccac atgccggccg ccaagctgct gaccggcaat gaacgccgtg ccgtggcggc
     2581 tgtcgatctg cgtccggcat cgggtttacc cgtcctggca tttctcggcg cgctggtgtt
     2641 ttcagtcctg tttttcaggc gtccgcgagt cattcccgct ttcggttcgt cagccctgtt
     2701 tccggctcgc cgtcctcccg tgccttcctg ggcacccgat ggggctaaat tcatccggcc
     2761 ccctccccgc attctctaat ctcttctcga ttcctgtttc tccgttcacc acagccgcaa
     2821 atccgtggtg agcggatgct gaccgccccc atttgcggtc aagccggttc ggctccgggc
     2881 ccactctcag gaaggtgtct cgccctctat gcgtacgcgt ctttggatcg tcatttctgc
     2941 cgtcatcggc gttgcagcga tttgtgttct cgcctggcgc ggacaccatc ccaccaacac
     3001 cgtggaagcc gctcccggtc cacctgcagc gcaggtcgcg ctggtccagc gcggcgacat
     3061 tgcccaggtg ctcaccctgg cgggacaatt ccagccctac caggtcgtcg atgtccatcc
     3121 caaagtctcc ggcttcatcc ggcacattta cgtcgacatc ggcgaccgcg ttcgccaggg
     3181 gcagactctc gccgtcctcg aagtcccgga gctgaaggcg cagctgcagg gcactatttc
     3241 tgaagtggcg cgcagcagcg acgagattgt tcgcgcacag cacgaagtcc ggcgcgccga
     3301 atccactcac gccgcactgc ataccgacta tcagcgcctg ctcgatacct caaaggcgca
     3361 gcccggactg gtcgcgcagc aggaactgga tgacgcgcag gcaaaagacc tcgcctcaga
     3421 ggccgatgtg gatgctgcca aagccgcgct ggccgcggcg gggaagggca tggaggtttc
     3481 ccgcgccgac aatgcgcgcg tgagcgcgat cgaggattac accaacgtca ttgccccgct
     3541 ggacggcgtc atcgtttggc ggtatgccga cacaggcgct ctgattcagg caggcatcag
     3601 ctcgaacaat caggatctgc cgatcgtgaa gctggctgag gcgaacgtga tgcggctgcg
     3661 tctgcccgtg cccgagacct atgtgcgcta cgtgcacata ggcgatcccg tcaaagtgcg
     3721 cgtcgatgcc ctgaaccgat ccttcaccgg caaggtcgtc cgctttacgc gtgagctgaa
     3781 tttcgaaacg cgaaccatgg aaaccgaagt cgacgtcgac aatcctgggc ttgccatcga
     3841 ctccggcatg tatgccaatg ccctgatgcg gctcgaccat gccgatcagg tgctgacggt
     3901 tccagtcggc gcagtcatcc tgcatggcga tcaggagcag gtgtatgtgc tggactccgg
     3961 gaaccatgtc tggatccgga atgttcacgt ggggatccgg ggcagccagc tcgccgaaat
     4021 tcagagcggc ctcaaaaagg gcgaccgcgt gattgtcgcg gcacaggcca aatacacctc
     4081 aggcgaaacc gtcaccccca ttgtggagaa gactgcggcg tcggaaacca accctcagtc
     4141 cggcgggatg atcgatatga atcccgaagc ggcctcggac aatggaggcc agcagtaaat
     4201 gccaaagttt gcgctgaagt atcccttctt catcctcatg ctgtgcctca tgatcacgat
     4261 cgttggagtc gtgaacctgt tcagcatgcc cgtcgatctg ttccccgaca tcgacatgcc
     4321 cgtggtggta gtggcgacgt tttacagcgg catgccgcct cagcagattg aagcagacat
     4381 tacgaacacg ttcgagcggt tcttcacgct ggcggccaac gtggaccaca gcgagtcgcg
     4441 ctcgctgacc ggcgtgagcc tcatcaagat ttatttcaag ccaggcacag atccgaacgc
     4501 agcgctgagc aacgtgtcga acctggcgat ggccgacttg cggcgactgc ctcccggcac
     4561 gctgccgccg gttgtgctgg ggctgaccgc gtccacgcag ccggtgtgcc tggtgacgct
     4621 cgaaggcaaa ggcctgaacg aaacccagct caaggatctg gcgcagttcc aggtccgcaa
     4681 ccagatttcc aacgttccag gcgcttctgt tccgcaaccc tatggaggca cctaccgcca
     4741 gatccaggtg tacgtcgatc cgctgaagat ggaggcgcgc aacctgagtc tcagcgacgt
     4801 tgtgcacgcc gtggacagct cgaatctgat cctgcctgcc ggcgacgtgc gcatcggtaa
     4861 caaagacttc aatatttacg cgaacagcca gtttccgacg gctgaggcga tgaaccaaat
     4921 gccgctgaag tcggtcggca atggctccgt tctcgtggcc gatatcggac atgccgtcga
     4981 tgcgggcgcg ctgcagtaca acatcgtccg catcaacgga caacgctcgg tctacgtgcc
     5041 ggtgttcaag cagggcggaa acagcaacac cctcacgatc gtggacggca tgaaacaggc
     5101 gatcaaacac ctggtcgata ttccggagtc cctgaagacc gcggtggtat tcgatcaggc
     5161 tgtttttgtg aagctcgcgc tcaagcagct ggtgaatgag gcgatcatcg ggctggtgct
     5221 caccggcatc atgattctgc tgtttctcgg cagcccgcgc gcgacggttg cggtgatgct
     5281 ggccgtgccg ctctcggcgc tggcgtgcat gctgatggcc aacatgctgg gcggcaccat
     5341 caacacgatg attcttggcg gcctcgcgct cgccttttcg cgcctgatcg acaacggcgt
     5401 ggtggtgctg gagaacatct tccgctacat ggaactgggc gagccgacgc gcatcgccgc
     5461 ggagaagggc ggcacggagg tttccctcgc cgtgcttgcg gccaccgtga cgacgtcgat
     5521 cgtcttcctg cccgtggttg ggctgtccgg tgtcagcaaa tatctcttca cgccgctcgc
     5581 gctgggcgtc gtgctctcga tgtttgcctc ctatgccttc gccatgaccg tgattccgct
     5641 gttttgcgcg ctgtttctga agggacacgg acacggggag cgcgatgaag caggagaact
     5701 cgtcgacaag cacgaagaac ctcgcaaacg ccgcttgggt ctggagccga tggttgcgaa
     5761 attcaacgag tacttccagc gcctggtgaa ccgatacgaa gctctcgtgc gaaagtcgct
     5821 gctgcgtcct ggactcacca ccgcggtgat ccttgcaggc tgcgccattc tgctggttgc
     5881 gaccttcccg ttcatggggc gcgcttactt tccgcgcacc gatccagggc agttcatcat
     5941 caacgtccgc atgccttccg gcacgcgcct cgaggtcagc aacgaggata ttgcccgcgt
     6001 ggagaagatc atccgcgaag aggtcgagcc gtccgatctg gacatgatcg tctcgaacat
     6061 cggcgtttat ccggacctct cttccatctt cacttcgaac gcgtcgatgg acacagcctt
     6121 cgtccagacg agtctgaagg aaggccactc tatcggcagc tatacctaca tgcggcgggt
     6181 gcaggagcgg ttgtctcgcg aaatgccgca gttgacgaca tactttcagg cgggcggact
     6241 gatcgatggc gtgatcaacc agggattgcc cgcacccatc gacctccagg tcagctcgag
     6301 aaacatggaa gatgcttacc agcttgctga aaagctggcg gcaaagattc gaacctttcc
     6361 caacgtcagc gctacctata ttccgcagga catcaactac cccggcatcc agctcaatat
     6421 cgatcgtgag aaggcctcgc tgattggcct ttccgcgaag gacgtggtcg ataacgtgat
     6481 cacagctctg acctcggacg gcatggtggc cccgacgtac tggatcgatc cgcatagcgg
     6541 caacaactac atggtcaccg tgcagtacgc gaatcactgg attcagaaca tgtcgatgca
     6601 ggacttcaag agcattcctc tgcgcggcac gcatccgccc ggcttcagtc cggtcgacgt
     6661 cgggcgaacc gcatccagct tttcagagga tatgatgagc gggtcgcacc gtgccggtta
     6721 cacgccgctc agttcggtgg cggacatccg tctgatcaac acgccgacgg aagtggatca
     6781 ctatcagatt cgccgggtca tcgacgttta tgtgtcgacg aagtcgga
//
LOCUS       DASZYN010000078         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_196366, whole genome shotgun sequence.
ACCESSION   DASZYN010000078 DASZYN010000000
VERSION     DASZYN010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196366"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2069
                     /locus_tag="VHE33_02075"
     CDS             <1..>2069
                     /locus_tag="VHE33_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HVT96264.1"
                     /translation="ERKRSMEELLLWLQHTPNLLDALCAIRRLPPTNYSNEQWTTLRN
                     VFTVLRQAVAELKVVFAEHNQVDFTELSLAAVQVLKDSPERVLDIAGNIRHLLIDEFQ
                     DTSRRQHQLISDLLAAWDPGEGRTVFLVGDPMQSIYMFRQAEVELFTQVREHGIGTTA
                     GQLRCQPVQLSVNFRSHAGLTEPLNGIFDLICAQKAQPGSASVQFARATSGAVGNPGQ
                     SGLHIHPQLIGAAHRNPTLDEIAAAQREEAEIVLNIVEQHLPQIRHARDAGGEYRVAI
                     LVRARNHLAQIVPLLRERTIPFRAVEIEHLSDRQELLDLRALTRALLHPMDRIAWLSV
                     LRAPWCGLTLSDLHLLTGSDDPTLRNKSVLELIESHSHLLSDDGRQRLDRTAGILRRA
                     LALRWRQSDSPSFASWIERTWRTLGGPACLDAAAYENAQVFFSLLDAVTPDGLAPSTA
                     SFDAEFDRLFAQPDPGVSERAGIQLMTIHKAKGLGFDVVIVPGLDRRSSGDPNPLVCS
                     LERINPWTEDSEFLVAPIGLHGEDTDPLYKWVRKQRQIRFDEERKRLLYVACTRARQE
                     LHLLGTAALTAAGVRVQNQDSLLATAWPSLRGAFEGALRQTAPAPKPSRVIAFPGEVR
                     EMAAVTETPRMKLRRLPLDAGSAAPRENVTVAGSFSVADPEAPEFQRREGSRESRLIG
                     STIHTFL"
BASE COUNT          363 a          705 c          606 g          395 t
ORIGIN      
        1 agagcgcaag cggtccatgg aggaactgct gctgtggctg cagcacaccc caaacctgct
       61 cgatgcgctg tgcgccattc gccgcctgcc gccaacgaac tacagcaacg agcaatggac
      121 tacgctgcgc aacgtcttca ccgttctgcg ccaggccgtc gccgagctca aggttgtctt
      181 cgccgagcac aaccaggtgg acttcacgga gttgtctctg gccgcggtgc aggtgctgaa
      241 agactctccc gaacgcgtcc tcgacattgc gggcaatatt cgtcatctgc tcatcgatga
      301 attccaggac acctcgcgcc ggcagcacca actgatctcc gacctcctcg cggcctggga
      361 tcccggcgag ggccgtaccg tctttctggt cggcgatccc atgcagtcga tctatatgtt
      421 tcgccaggcc gaggtggagc tgttcaccca ggtgcgcgaa cacggcatcg gcacaaccgc
      481 cggccagctt cgctgtcaac cggtgcagct ctccgtcaac ttccgctcgc atgcgggact
      541 gactgagccg ctgaacggca tttttgacct gatttgcgcc cagaaagcgc agcccggctc
      601 tgcctccgtc cagtttgcgc gggcgacctc cggtgcggtc ggcaatccag gacaaagcgg
      661 actacacatc catccgcagc tcattggcgc ggcgcatcga aacccaacgc tggacgaaat
      721 cgcagccgcg caacgcgaag aagccgagat cgtcctcaac atcgtcgagc agcacctccc
      781 gcaaatccgc cacgcgcgag acgccggtgg tgaataccgc gtcgccatcc tggtccgcgc
      841 gcgcaatcat ctggcccaga tcgtgcctct cctgcgggag cgcacaattc cgtttcgcgc
      901 tgttgaaatc gagcatctgt cggatcgcca ggaactgctc gatctgcgcg cgctgacccg
      961 cgcgctgctg catcccatgg accgcatcgc ctggctctcg gtgctgcgcg cgccatggtg
     1021 tggactcact ctgtccgatc tgcatctcct caccggttcc gacgatccca ccctccggaa
     1081 caaatccgtc ctcgaactca ttgagtcgca cagccatctg ctctccgacg atggccggca
     1141 gcggctcgat cgcaccgcag gaattttgcg ccgtgctttg gccctgcgct ggcgccagtc
     1201 cgactcaccc tcgttcgcgt cgtggattga gcgcacctgg cggaccctgg gcggcccggc
     1261 gtgtctcgac gcggccgcat acgagaacgc ccaggttttc ttctcgctgt tggacgctgt
     1321 aacaccggac ggcctggctc cttcgacggc aagctttgac gccgagttcg atcgcctgtt
     1381 tgcgcagccg gatccaggcg tcagcgagcg ggccggcatc cagttgatga cgattcacaa
     1441 agccaaaggc ctcggcttcg acgtcgtcat cgttcccggt ctcgatcgcc gctcatccgg
     1501 cgatcccaat ccgctggttt gttcgctcga gcgcatcaat ccatggacgg aggacagcga
     1561 attcctcgtc gccccgatcg gtcttcacgg agaagataca gatcctctct ataagtgggt
     1621 gcgcaagcaa cgtcagatcc ggttcgatga agagcgcaag cgcctgctct acgtggcctg
     1681 tacgcgggcg cgacaggaac tgcatttgct ggggacggct gcgctcaccg ctgccggcgt
     1741 gcgcgtgcaa aaccaggata gtctgctggc cacggcgtgg ccgtctctgc gcggtgcctt
     1801 cgagggtgcg ttgcggcaaa cggctcccgc cccgaagccg tcccgggtca tcgcctttcc
     1861 aggcgaggtt cgcgaaatgg ctgccgtaac cgagactccg cggatgaaac tgcggcggct
     1921 tcccctggat gccggctcag cggcgccacg agaaaatgta acagtagcag gatcattctc
     1981 cgttgccgat ccggaggcgc ccgagtttca gcgacgcgag ggttcgcgcg agtcgcgcct
     2041 gattggttcc accatccaca cgttcctcg
//
LOCUS       DASZYN010000079         6648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_965583, whole genome shotgun sequence.
ACCESSION   DASZYN010000079 DASZYN010000000
VERSION     DASZYN010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965583"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2025
                     /locus_tag="VHE33_02080"
     CDS             <1..2025
                     /locus_tag="VHE33_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HVT96265.1"
                     /translation="AALSSASGENYTAITLPFARFHFTDGDAGIEFAVENTRWQCDLH
                     TWTCHSHPAFSGDDYGYDDTPRPENSAEKTLASPDGKWLACILNDNVEVRSKDGKQKY
                     TLSHDGSEGDFYAFSTMAWSPDSTHLVAYRIRPGYRRVIQYVESSPKDQLQPESSTMV
                     YPKPGDVLPLPQPVLFDVAAHKQMDISNTLFPNPYDLTHAVWWKDSRGFTFEYNQRGH
                     QVYRVIEVDGHTGVPRTLLDETSSTFVNYEPLVANQFDTGKKYRHDIHDGQEIVWASE
                     RDGWEHLYLFNGHTGALKNQITRGHWVVRAVNYVDDDKRQIWFEASGMNPNEDPYFVH
                     AYRINFDGSGLTPITPDEANHYVEFSPDGKYYLDTWSRVDLAPTMALYQVSDNKQLMV
                     VEHADIGKLVAAGWHAPDVFHAKGRDGATDIWGVIYKPQGFDAKKKYRVIEDIYAGPQ
                     GSFVPKTFTTHIEPLTQLGFVVVQIDGMGTNNRSRAFHDVAWKNLKDAGFADRILWHK
                     AAAAQFPWYDISDGVGIFGTSSGGQSSMGALLFHPDFYTVAVSNSGCHDNRMDKIWWN
                     EQWMGWPIGQQYSDSSNVDNAWRLKGKLLLIFGEMDHNVDPSSTLQVVDRLIHAKKTF
                     DLLEVPGADHGTRGPYAYYTQRKTYDYFVRNMLNEVPPDWNVDPVEASGN"
     gene            2260..2976
                     /locus_tag="VHE33_02085"
     CDS             2260..2976
                     /locus_tag="VHE33_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT96266.1"
                     /translation="MRPPTASDHPTIIRVRNLRKEYLTGRGRLTLFDGLSFDIVRGEM
                     LAIVGQSGAGKSTLLHILGALDTPSAGDVYCASIQLGSLTPGQAAAFRNREIGYVWQF
                     HYLLPEFTALENVAMPLLARGESKASAFRSAEQWLNEVELGARVTHRAGELSGGEQQR
                     VSIARALITKPKVLLADEPTGDLDSLTAEMIFNLINRLHNEHRLTSVIVTHNQALARR
                     CTRILRLEKGCLNEVAPLAV"
     gene            3255..5711
                     /locus_tag="VHE33_02090"
     CDS             3255..5711
                     /locus_tag="VHE33_02090"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HVT96267.1"
                     /translation="MFERYTEKARRVIFFARYEASQFGSPYIETEHLLLGLLREDKAL
                     TNRFLRSHASVESIRKQIEGHTTIREKVSTSVDLPLSNECKRVLAYAAEEAERLSHKH
                     IGTEHLLLGLLREEKCFASEILHERGLKLLAVREELARATQEKVPQQQRNRESSLLAE
                     FSRDLTQAAMDSQLDPLVGRDQELERVVQILCRRTKNNPVLIGEPGVGKTAIVEGLAQ
                     RISDGDVPSFLADKRVLALDLSLIVAGTKYRGQFEERLKTIMKELMENQNSIIFIDEL
                     HTLVGAGSAEGSLDAANILKPALSRGEIQCIGATTPAEYRKSIEKDRSLERRFQAVKV
                     PPPNEEDAIKIINGIKDRYEKFHAVSYTDEAINFAVSHSNRYISDRFLPDKAIDLIDE
                     AGARVKLRQTSLPEEITEVQKRIKFIVHRMENAIANHEFEKARFYSDEERKERDNLRS
                     LRDKYHLDDSTAGIVGREDIEDVVSRWTGVPITSIKEEETQKLLRVEEELHKRVISQD
                     KSISALARAIRRSRAGLKSPHRPIGSFLFLGPTGVGKTEMARTLAQFLFGSEKSLIRF
                     DMSEFMEKHSVSKLIGSPPGYVGYEEGGQLTERVKRSPYSVVLLDEIEKAHADVFNIL
                     LQVFEDGQLTDGLGNTVDFKNTIIIMTSNIGARHLQRRQGLGFQSDREEMVMEKIEDL
                     VKNEVKRTFNPEFLNRIDEIILFQSLTDADLIQILELLVQQLNANLAQKAITISVTDD
                     AKKWILDKTLVDRSYGARPLRRALQRYVEDPLSEALIAGGINERPAFLEVYLDNNQLF
                     YRPVAKDGEEKATEGILLVN"
     gene            complement(5782..6369)
                     /locus_tag="VHE33_02095"
     CDS             complement(5782..6369)
                     /locus_tag="VHE33_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96268.1"
                     /translation="MKKTAIGVAAVWVAVAVGLLVPPSLRGQDTAKASGAEATAEKHF
                     YRLNLVVEELNEGGKIINSRNYLATIGSGAREVPGFTDQHIRTGSRIPILTGSKDGGT
                     AFQYVDVGVNFDVRQVTEIGDKVSFRLSAEISSLADDNSGGASGNLRGDPVIRQSRWD
                     SSVLIPVGKPTIVFSADDLDSKGKMQVEVTATKVE"
     gene            complement(6375..>6648)
                     /locus_tag="VHE33_02100"
     CDS             complement(6375..>6648)
                     /locus_tag="VHE33_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96269.1"
                     /translation="GGRREAGLARTEERNPLPKQDVFPEPRPLSAQEEALVAFASHVP
                     AKVQEQVIEAQKHANDPIVIAELKIAPLEIGDGEQDSGQQEKNKEK"
BASE COUNT         1405 a         2143 c         1758 g         1342 t
ORIGIN      
        1 gctgcgcttt cttctgccag cggcgagaac tacacggcga tcacactgcc ctttgcgcgc
       61 tttcatttca ctgacggtga cgccggcatc gaattcgccg ttgagaacac tcgctggcaa
      121 tgcgatctgc acacctggac ctgccatagt catccggctt tctcgggtga cgactacggc
      181 tacgacgaca cgccgagacc ggaaaacagc gccgagaaaa ccctcgcctc gcctgatggc
      241 aaatggctgg cctgcatcct gaatgacaac gtcgaggtcc gctccaaaga tggaaagcag
      301 aaatacaccc tgagccacga cggctccgaa ggcgacttct atgccttctc tactatggcc
      361 tggtcgccgg attcaacgca tctcgtggcc taccgcatcc ggccagggta tcgccgggtc
      421 attcaatacg tagaatcctc gcccaaagac cagttgcagc cagaatcttc caccatggtg
      481 tacccaaaac ccggggatgt cctgccgctg cctcagccgg ttcttttcga cgtcgcggct
      541 cacaaacaaa tggacatcag caatacgctc tttcccaatc cctacgatct gacccacgcc
      601 gtctggtgga aagacagccg cggcttcacc ttcgaataca atcagcgcgg ccatcaggtc
      661 taccgtgtca ttgaggtaga cggacacacc ggcgtgcccc ggaccctcct cgacgagacc
      721 agcagcacct tcgtcaacta cgagcctctg gtcgcgaatc aattcgatac cgggaagaaa
      781 taccgccacg acatccatga cggccaggag atcgtctggg cttcagagcg cgatggttgg
      841 gaacatctct atctcttcaa cggacacacc ggcgctctca aaaaccagat cacccgcgga
      901 cactgggtcg tccgcgccgt caattatgtc gacgatgaca agcgccagat ctggttcgaa
      961 gccagcggca tgaaccccaa tgaagatcca tacttcgtcc acgcttatcg catcaacttc
     1021 gacggttccg gcctcacgcc aatcacgccc gatgaggcga accactatgt cgagttctca
     1081 ccggacggca agtactacct cgacacctgg tcccgggtcg atctcgctcc aaccatggcg
     1141 ctctatcagg tctcggacaa caagcagctc atggtcgtcg agcatgccga catcggcaag
     1201 cttgttgccg ccgggtggca cgcgcccgat gtcttccacg ccaaaggccg cgatggcgcc
     1261 acggatatct ggggtgtcat ctataaaccg cagggcttcg acgcgaagaa gaaataccgc
     1321 gtgatcgagg acatctatgc gggtcctcag ggctcgtttg ttcccaagac cttcacaact
     1381 catatcgagc cgctaaccca actcggattc gttgtggttc agatcgatgg catggggacc
     1441 aataatcgct cccgggcctt ccacgatgtg gcctggaaga atctgaagga cgcggggttt
     1501 gccgatcgca tcctgtggca caaagcagcg gccgcccagt tcccctggta tgacatctcc
     1561 gacggcgtcg gcatcttcgg cacttcatcc ggaggccaga gttcgatggg tgcgttgctc
     1621 tttcatcccg atttctacac cgtcgccgtc tccaacagcg gctgccacga caaccgcatg
     1681 gacaagatct ggtggaacga acaatggatg ggctggccca tcggtcagca gtactcggac
     1741 tcctccaacg tcgacaatgc ctggcgtctc aaaggaaagc tgctgctgat ctttggcgag
     1801 atggatcaca acgtcgatcc ttcttctacc ctgcaggtgg tcgatcggct catccacgcc
     1861 aaaaagacct tcgaccttct ggaagtgccg ggcgcagacc acgggacccg cggtccttat
     1921 gcctactaca ctcagcgcaa aacgtacgac tatttcgtcc gcaacatgct gaacgaagtt
     1981 ccgccggact ggaacgtcga tcctgtcgag gcatctggaa attgacgcag cgaccctgat
     2041 caggatcggg ccgagcggaa ttggctcgat cgaagacgtc tttttgcgcc actccctcct
     2101 tcgtaattgc tgtttccggt actcctgtac cggttttcct ccagagtgcg tccaatccag
     2161 taactcccat cttccctttt gggtagggga gtggtacagg taggtatgga tacggttcgc
     2221 gccacggaag cactacccca gagcccagta gagctcggtt tgcgacctcc gaccgcctcc
     2281 gatcatccaa ccatcatcag agtcaggaac cttcgcaagg agtaccttac cgggcgaggc
     2341 cggctcaccc tgtttgacgg attgtcattc gacatcgttc gcggcgagat gctcgccatt
     2401 gtgggtcagt ctggcgccgg aaagagcacc cttttacata ttctgggcgc gcttgatacc
     2461 ccctccgcgg gcgacgtata ctgcgcctca attcagttgg gttcgctcac acctggacag
     2521 gcagccgcat tccgcaaccg cgaaatcggc tacgtctggc aattccatta cctcctgccg
     2581 gaattcaccg cgctggagaa cgtcgcgatg cccctgctcg ccaggggcga gtcgaaggcc
     2641 tcggccttca gaagtgcgga gcagtggttg aacgaagtcg aactcggcgc ccgggtcact
     2701 catcgtgccg gagaactctc cggcggtgag cagcagcgcg tctcgatcgc ccgcgccctc
     2761 atcacgaagc ccaaagtgct ccttgccgat gagccaacgg gcgatctcga cagcctcacc
     2821 gccgagatga tcttcaatct gatcaatcgt ctgcacaatg agcatcggct cacgtcggtg
     2881 attgtcaccc ataaccaggc gctggcgcgc cgttgcacaa ggattttgcg gctcgagaag
     2941 ggctgcctga acgaagttgc tccgttggct gtctagcaac ggatcgttgg aagtcgggtt
     3001 tgtaagatca gcggaaacgc cgtcgaatgt tcgatactgg cagagggacg gcgctctcgc
     3061 aaagtgggat ggatgcggac gaacgcacca gcctcagggc cgcccgcgat ttggccccag
     3121 atttctggca agggcggcaa ttcgagtctc agccatgttg aggctcataa ggaacgaaga
     3181 gtcccgaatg ccccgggctt gccggcactg acgactggca tccctccagg caagcggagc
     3241 ctctcagagg gatcatgttc gaacgctata cagaaaaggc acgccgcgtc atcttcttcg
     3301 caagatatga ggccagccag ttcggctctc catatatcga aaccgaacac cttctcctcg
     3361 ggcttctgcg cgaagacaaa gcgcttacca accgcttcct tcgctctcat gcatcggtcg
     3421 aatccattcg caagcagatc gaaggccata ccacgattcg tgagaaggtc tcgacctccg
     3481 tcgaccttcc gctatcgaac gaatgcaagc gcgtgctcgc gtacgccgct gaagaggccg
     3541 agcgcctgtc gcacaagcat atcggcaccg aacatctgct ccttggcctg ctgcgcgaag
     3601 agaaatgctt cgcctccgaa atcctgcacg agcggggcct gaagctcctc gctgttcgtg
     3661 aagaactggc gcgcgccacc caggagaaag ttccgcagca gcagcgcaac cgcgagtctt
     3721 ccctgctggc ggagttctct cgggacctca cccaggctgc tatggactcc cagctcgatc
     3781 ctcttgtcgg tcgcgatcag gaactggagc gcgtggtaca gatcctgtgc cgccggacga
     3841 agaacaatcc ggtgctgatc ggcgagccag gcgtgggcaa gactgccatc gtcgagggtc
     3901 tcgcgcaacg catctccgac ggcgatgtcc cgagcttcct cgccgataaa cgggtcctgg
     3961 ctcttgattt gtcgctgatc gtcgccggca ccaagtatcg cggccagttc gaagagcgtc
     4021 tcaagaccat catgaaagag ctgatggaga atcagaactc catcatcttt atcgacgagc
     4081 tgcacaccct ggtcggcgcc ggctccgcag aaggctcgct tgacgccgcc aacattctca
     4141 aaccggcgct ctcccgcggc gaaatccagt gcatcggcgc caccaccccg gccgaatatc
     4201 gcaagtcgat cgagaaagac cgatcgctcg agcggcgctt ccaggcggtc aaggtgccgc
     4261 cgcccaacga agaagacgcg attaaaatca tcaacggcat caaggaccgt tacgagaagt
     4321 tccacgccgt cagctacacc gatgaggcca ttaacttcgc tgtctctcac tccaaccgct
     4381 atatctccga tcgtttcctg cccgataagg ccatcgacct gatcgacgag gccggcgctc
     4441 gcgtgaagct gcgccagact tcgctgccgg aagagatcac cgaagtccag aagcgcatca
     4501 agttcatcgt gcaccgcatg gagaacgcca ttgcgaacca cgagttcgaa aaggcgcgct
     4561 tctactcgga cgaggaacgc aaggaacgcg acaatctccg ctccctgcgc gacaagtatc
     4621 atctcgacga ctccacggcc ggcattgtgg gccgcgagga catcgaggat gtggtcagcc
     4681 ggtggaccgg cgtgcccatc acctccatca aggaagaaga gacgcagaag ctgctgcgcg
     4741 tcgaagagga gctgcataag cgcgtcatca gccaggacaa atccatctcg gctttggctc
     4801 gtgcgatccg gcgctcgagg gccgggctca aatcgccgca ccggccgatt ggatctttcc
     4861 tgttcctcgg acccacgggc gttggcaaga ccgaaatggc tcgcaccctt gctcagttcc
     4921 tgttcgggag cgagaaatcc ctgatccgct tcgatatgtc ggagttcatg gagaagcact
     4981 ccgtctccaa gctcatcggc tcgcctccgg gatacgtggg ctacgaggaa ggcggccaac
     5041 tcaccgagcg tgtgaaacgt tcgccctact ccgttgtcct gctcgacgaa atcgagaagg
     5101 cgcacgccga cgtctttaat atcctgctgc aggtatttga agacggccaa ctcaccgacg
     5161 ggctcggcaa cacggtcgac ttcaagaaca cgatcatcat catgacctca aacatcgggg
     5221 cccgtcatct ccagcggcgc caggggctcg ggttccagag cgatcgcgaa gagatggtca
     5281 tggagaagat cgaagacctc gtgaagaacg aggtgaagcg cacgtttaac cccgaattcc
     5341 ttaaccgtat cgatgagatc atcctcttcc agtcgctgac cgatgccgac ctgatccaga
     5401 ttctcgagct gctggtccag cagctcaacg cgaacctggc gcagaaggcc atcacgattt
     5461 ccgtcaccga tgacgcgaag aagtggatcc tcgacaagac tctggtcgat cgcagctatg
     5521 gagcccgtcc gctccgccgc gcgctccagc gctatgttga agatccgctg tcggaagcgc
     5581 tcatcgccgg cggcattaac gagcgacctg cgttcctcga ggtctacctc gataacaacc
     5641 agctcttcta ccggccggtg gcgaaggacg gcgaagagaa agcgaccgaa ggcatcctgc
     5701 tggtcaacta accagctcag gctgaattaa aaggctgcgg attcgtccgc agccttttaa
     5761 tttcgaacct tgctttgctc ctcactccac cttcgttgcc gtcacctcca cctgcatctt
     5821 ccccttgctg tccagatcgt cggccgaaaa cacgatcgtc ggcttcccta ccgggatcag
     5881 cacagatgaa tcccaccggc tctgccggat caccggatca ccccgcagat ttcctgacgc
     5941 cccgccggaa ttgtcgtctg ccaggctgct gatctcggcc gacagacgga agctcacctt
     6001 gtctccgatc tccgtgacct gccgcacgtc gaagttcact ccaacatcca catattggaa
     6061 cgctgtgcct ccgtccttcg aacccgttaa gatcggaatc cgggaacccg tacggatgtg
     6121 ttgatcagta aacccaggca cctctcgtgc tcccgaccca atcgttgcca ggtaattccg
     6181 cgagttgatg atctttcccc cctcgttcaa ttcctccact acgaggttta aacgatagaa
     6241 gtgcttctct gccgtcgcct ctgctcccga cgcttttgca gtatcctgcc cccgcaaact
     6301 cggcggcacc agcaatccga ccgctactgc cacccacact gccgccacgc caatcgctgt
     6361 tttcttcata cctttcattt ctccttgttc ttttcctgtt gtccggaatc ctgctccccg
     6421 tcgccaatct ccagcggcgc aatcttcaac tccgcgatca caatcggatc gttcgcgtgc
     6481 ttctgggcct cgattacctg ctcctgcacc tttgccggca catgacttgc gaacgcgacc
     6541 agtgcctctt cctgtgcgct cagtggtcgc ggttccggaa atacgtcctg cttcggcaat
     6601 ggatttcgct cttctgtcct tgccaggccc gcctcccgtc ttccgccg
//
LOCUS       DASZYN010000080         3684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_132343, whole genome shotgun sequence.
ACCESSION   DASZYN010000080 DASZYN010000000
VERSION     DASZYN010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132343"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..1038
                     /locus_tag="VHE33_02105"
     CDS             256..1038
                     /locus_tag="VHE33_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HVT96270.1"
                     /translation="MSAQAITWNLGMLGFPARGASRAEVIVPGQDGASSGRKYRAPGA
                     DRWQRKEQERGAERVGEKPVEAEWQVLVERCLKGDSFAWTELVKAHHRRVYGLCFRFT
                     GSSTDAEDLTQEVFLKVYGNLTSFDLERGTFQTWITTMTRNLLVDHFRRSRAQRVTDS
                     MDAGWEGDGDDIPMLQRLADKGPTQHDRAVQKEIEKMVQEALTRISPELREAVILRDL
                     QDMDYKDIAKVLRIPEGTVKSRISRGRAELARLLERNKGQVV"
     gene            1039..2028
                     /locus_tag="VHE33_02110"
     CDS             1039..2028
                     /locus_tag="VHE33_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HVT96271.1"
                     /translation="MADERQFGKKPGTPGSAMRCEEWEALLTDALDGLLPAGKAAAFA
                     AHAESCENCGDLLAHVKQGQEWLTYLHEEPEIPSGLVAKIVEKTLGAGGVPIPVVAAG
                     VPATAGGAVAMAMPWRRSFHETRLLMTVAMAFFSLALTLNIITGGHLTSLKLADLTPS
                     QIGSTLSREFYGARGSVVKYYDNMRLFYRLQTRMRELRRDVEQPQQQQNNQQQNQQQK
                     PSGRTGGSASVQQGTGLRAQVTGQRSAFSARRSGELQRSVLSVQRSGELQRSAFGDWR
                     SEELQRSVGSGEQTEQKQEAAVVVLKSDQTGTSISLGRSETGLRQTNVERGLV"
     gene            2025..3206
                     /locus_tag="VHE33_02115"
     CDS             2025..3206
                     /locus_tag="VHE33_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="B-box zinc finger protein"
                     /protein_id="HVT96272.1"
                     /translation="MNCVNHPEAAVTAFCQNCGKGLCAQCSREVQGNVFCEPCLVARV
                     TGAVPPGAGMPGPGMPGPGVANPYGPPPAGVPNPGTATILGFIPGVGAMYNGQYIKAI
                     VHVLVFVVLISITEHYGIFGIFVGAWVLYQVFDAHQTAKARRDGLPLPDPFGLNELGN
                     AFGGHAPRPYTPPVTPPPQGGAVPPPGFVTGAVPGAAPGVPGQPYADWAEQVRQQAHE
                     FGRQSAEWGEQLRQRIVAETQAGARPYNPTGGIGGPGSWSAPPPPPGFVAPGVPPGAG
                     YVPPGVPPNMPPGWVPPPPEIELERARREPIGAIVLILLGMLFLFNTLGFFNFGWVHH
                     GWPLIIVGIAVWLLFRNSGRFGFYGRTGPPPVAPGAPGNVPPPPPAASATPEPPAGGG
                     Q"
     gene            3203..3541
                     /locus_tag="VHE33_02120"
     CDS             3203..3541
                     /locus_tag="VHE33_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96273.1"
                     /translation="MNQYLFLRRMRGPIILLTFGITAILDEYAGISYGHSWPLYIIVW
                     GLLKLAENAILAQNPPATPAPWPGYQAGYPTPGSTGATTYTGTVAGETVSTATIVPPP
                     DNIAREEGGR"
BASE COUNT          694 a          975 c         1400 g          615 t
ORIGIN      
        1 gacgcgcgac tccccacatg cccaactgca ggcaagtggg gcacccaggc agggtgcagc
       61 gagtcagcga gtcagcaaaa cgcgagcgga agtggagaga aagtcgagcg aacgtcgagt
      121 gtcacacccg taagacggct cgatcagggg atccctggtc ggattggtcc tgaagaattg
      181 gcctgcgggg tcggacggcg ggtaagacgg ctcaaattcc ggaactaagc gccatttgga
      241 tgcgtattat tgcccatgag cgctcaagcc atcacgtgga atctgggaat gctgggattc
      301 cccgcccggg gggcatcgcg ggcggaagtg attgtgcctg ggcaggatgg ggcgtcctcg
      361 gggcggaaat accgggcgcc gggcgcggac cggtggcagc gcaaagagca ggaacgaggc
      421 gcggagaggg tcggagaaaa gcctgtggaa gcggagtggc aggtcctggt ggagcggtgc
      481 ctgaagggcg acagcttcgc gtggaccgaa ctggtgaaag cgcatcacag gcgagtgtat
      541 ggtttgtgct tccggtttac cggatcgtcg accgacgccg aagatctgac ccaggaagtt
      601 ttcctgaagg tgtacggcaa tctgaccagt ttcgatctgg agcgcgggac gtttcagacc
      661 tggatcacga cgatgacgcg caacctgctg gtggaccact tccggcggtc gcgggcgcag
      721 cgggtgaccg attcgatgga cgcgggctgg gagggggatg gggacgacat cccaatgctg
      781 cagcggctgg ccgataaggg gccgacgcag cacgatcggg cggtgcagaa agagatcgag
      841 aagatggtgc aggaggcgct gacgcgcatt tctcctgagt tgcgggaagc ggtgatcctg
      901 cgcgatttgc aggacatgga ttacaaggat attgcgaagg tgttgcggat ccccgagggg
      961 accgtgaagt cgagaatcag ccggggccgt gcagaactcg cgcggctgct ggaacgtaat
     1021 aagggacagg tggtttaggt ggcagacgag cgccaattcg gaaaaaagcc gggcaccccc
     1081 ggcagcgcga tgcgctgcga ggagtgggaa gccctgctga cggatgcgct ggacggtctg
     1141 ctgccggccg ggaaggcagc ggccttcgcc gcgcatgcgg agagctgcga gaactgcggc
     1201 gatctgctgg cgcatgtgaa gcaggggcag gagtggctga cgtatctgca cgaggagccg
     1261 gagattccgt cgggcctggt cgcgaagatt gtcgagaaga cgctgggcgc gggcggcgtg
     1321 ccgattccgg tggtggcggc gggagtgccg gcgacggcgg gcggtgcggt ggccatggcg
     1381 atgccgtggc ggcggagctt ccacgagacg cggctgctga tgacggtagc gatggcgttc
     1441 ttttcgctgg cgctgacgct gaatatcatc acgggcgggc atctgacgag tctgaagctg
     1501 gcggatctga cgccctcaca gatcgggagc acgctgtcgc gggagttcta cggagcgcgc
     1561 gggtcggtgg tgaagtacta cgacaacatg cggttgttct atcggctgca gacgcggatg
     1621 cgggaactgc ggagagatgt ggagcaaccc cagcagcagc agaacaacca gcagcagaac
     1681 cagcagcaga agccgtcggg caggacgggc ggcagcgcga gcgtgcagca gggcacaggg
     1741 ctcagagcac aggtcacagg acagcgttcg gcgttcagcg ctcggcgttc gggagaactg
     1801 cagcgttcag tgctcagcgt tcagcgttcg ggagaactgc agcgctcggc gttcggcgat
     1861 tggcgctcgg aagaactgca gcgttcagta ggtagcggag agcagacaga acaaaagcaa
     1921 gaagcagcgg tggtggtatt gaagtcggac caaactggca cgtcaatttc cctgggtcgt
     1981 tcagaaaccg ggctgcggca aacgaacgtg gaaaggggcc tggtatgaac tgcgtgaatc
     2041 atcccgaggc ggcggttacc gccttttgtc agaactgtgg gaagggtctg tgcgcacagt
     2101 gctcgcgcga ggtgcagggc aacgtgttct gcgaaccttg ccttgtggcg cgcgtgacgg
     2161 gcgcggttcc gcctggggcg ggcatgccgg gaccggggat gccggggcct ggggtggcga
     2221 atccttatgg tccgcctcca gccggggtgc cgaatcctgg gacggcgacg attctgggat
     2281 tcattcccgg agtgggcgcg atgtacaacg gccagtacat caaggccatc gtgcacgtgc
     2341 tggtgttcgt ggtgctgatc agcatcacgg agcactacgg gatctttgga atctttgtag
     2401 gcgcgtgggt gctgtatcag gttttcgacg cgcaccagac ggcgaaggcg cggcgcgatg
     2461 gtctgccgtt gccggatccg ttcgggctga atgagctggg caatgcgttt ggcggacatg
     2521 caccgcggcc gtacacgccg ccggtgacgc cgcctccgca ggggggggcg gtacctcctc
     2581 cgggatttgt gactggagca gttccaggag cagcgcccgg agttccgggc cagccgtatg
     2641 cggactgggc ggagcaggta cggcagcagg cgcatgagtt tgggcggcag agcgcggagt
     2701 ggggagaaca gctgcggcag cggatcgttg cggagaccca ggccggggct cgtccctata
     2761 acccgacggg agggattggc gggccgggca gctggagtgc gcctccgccg cctccgggat
     2821 ttgtggcgcc gggcgttcca ccgggagcgg gctacgtgcc accaggtgtt ccgccgaata
     2881 tgccgccggg ctgggtgcct ccaccgccgg agatagagct ggagcgggcg cgcagagagc
     2941 cgattggggc gattgtcctg atcctgctgg gcatgctgtt cctgttcaac acgctgggat
     3001 tttttaactt cggctgggtg caccacggat ggccgctgat cattgtgggc attgcggtat
     3061 ggctgctgtt ccggaactcg gggcgattcg ggttttacgg aaggacgggg ccgccgcctg
     3121 ttgctcctgg agcgccgggg aatgttccgc cgccgccacc ggctgccagc gcgacaccgg
     3181 agccaccggc agggggtggg caatgaacca gtaccttttt ctgcggcgca tgcgggggcc
     3241 gatcatcctg ctgacgtttg ggatcacggc gattctggat gagtatgcgg ggatctcgta
     3301 cgggcacagc tggcctctgt acatcattgt gtggggactg ctgaagctgg cggagaacgc
     3361 aatcctggca cagaatcctc cggcgactcc ggctccatgg cctggttatc aggcgggata
     3421 tccgactccg gggagcacgg gagcgacgac gtacaccggg accgtggcgg gcgagacggt
     3481 gtcgacggcc acgattgtgc cgccgccgga caacattgca agggaagaag gagggaggta
     3541 gacagatggc atccacacct ccatcctgga catcgcggga agcgcggtgg caggccaaac
     3601 aggcagcgcg agcgcagcgg gcacagtgga aggcgcagat gcgcgcgcag aaggactact
     3661 accggagcta ctggcgcgga tggc
//
LOCUS       DASZYN010000081         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_68319, whole genome shotgun sequence.
ACCESSION   DASZYN010000081 DASZYN010000000
VERSION     DASZYN010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68319"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VHE33_02125"
     CDS             <1..261
                     /locus_tag="VHE33_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96274.1"
                     /translation="WNVLYHLPFMTSILRFFGNMFVRQDQVTMIQQAEGLKFDPSLML
                     IDDADKPAKWYFALKQARLDAEKTGTPMKHPLDGPVTLRWRS"
     gene            complement(280..>1611)
                     /locus_tag="VHE33_02130"
     CDS             complement(280..>1611)
                     /locus_tag="VHE33_02130"
                     /inference="COORDINATES: protein motif:HMM:NF023478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HVT96275.1"
                     /translation="RASRDAFFEQHALYAFNEEERTRSLMLMELERHAMLMYTSCGWF
                     FDDISGIETVQIIAYAGRVLQLASYLFGKRAAGLEKNFVTRLAEAKSNLPEQKDGAAV
                     YNRYVRSMEVGLEQVAAHYAISSIFTSYPDEADIFSYSVRRLAWETVVSGRVRLVVGQ
                     ALICSKITEAAETVAFGVLHFGDHNITAVVKRFDPTDSADYDQFVEEAKTAVTLGDFP
                     EVVRCFDRHFGNQTYSLRSLFRDEQKRILEILLTGTLEEVESSLSSIYDNQASLLHFL
                     SQSKLPRPQALTVAATFAINAGLRRALEAEPVDAIQARTWLGLAKSDEVALDKQLIGY
                     IAEQRMTRAMLALHARKEEMPLVEDALAIARTLRELPFDVNLWQAQNLWYDTWRHHRE
                     KPPSADWTEKMKELGTQMRIAVDAIVVEDGNGNGNHTQAPPLSPEVTSATA"
BASE COUNT          296 a          485 c          522 g          308 t
ORIGIN      
        1 tggaacgtcc tctaccacct gcccttcatg acctcgatcc tgcgcttctt cggaaacatg
       61 ttcgtgcgcc aggatcaggt cacgatgatc cagcaggccg aaggcctgaa gttcgatccg
      121 tcactcatgc tgatcgacga cgccgacaag ccggcaaagt ggtacttcgc cctcaagcag
      181 gcgcggctcg atgcggagaa aacgggaacg ccgatgaagc atccgctgga cgggcccgtg
      241 acgctgcggt ggagaagctg acggagggtg cggaacaggt tacgccgtgg cgcttgtcac
      301 ttcgggagac agcggcgggg cctgggtgtg attgccattc ccattcccgt cctcgaccac
      361 aatcgcatcg acggcaatgc gcatctgcgt cccgagttcc ttcatcttct ccgtccagtc
      421 ggccgacggt ggcttctcgc ggtgatggcg ccaggtgtcg taccacaggt tctgtgcctg
      481 ccacaggttc acgtcgaagg gcagctcccg cagcgtccgc gcgatggcca gagcgtcttc
      541 gaccaggggc atctcttctt tgcgcgcatg aagagccagc atggcgcgcg tcatccgctg
      601 ttcggcgatg tagccgatca gctgcttatc gagcgcgacc tcatcggact tggccaggcc
      661 caaccaggtg cgcgcctgga tcgcatcgac cggctcggct tcgagcgctc gtcgcaggcc
      721 cgcattgatg gcaaaggtgg cggcgacggt taaagcctgg ggccgcggca gcttgctctg
      781 gctcaggaag tgcagcagcg aggcctgatt gtcgtaaatg gaagacaggc tcgactccac
      841 ttcttccagc gttccggtaa gcaggatttc gaggatgcgc ttctgctcgt cgcggaacag
      901 cgagcgcaac gaatacgtct ggttgccgaa gtggcggtcg aagcagcgca caacctcagg
      961 gaaatcgccg agcgtgacgg cggttttggc ttcttcgacg aactggtcat agtcggccga
     1021 gtccgtggga tcgaagcgct tcacgaccgc agtgatgttg tgatcaccga agtggagcac
     1081 gccgaaggcg accgtttccg cggcttcggt gatcttcgag cagatcagcg cctgccctac
     1141 gacgagccgc acgcgcccgg agaccaccgt ctcccaggcg aggcggcgga ccgaatagct
     1201 gaagatgtcc gcttcgtcgg gatagctggt gaagatggag ctgatggcat agtgcgcggc
     1261 gacctgctcg aggccgactt ccatcgaacg cacatagcgg ttgtagacgg cggcgccgtc
     1321 cttctgctcg ggaagattgc ttttggcttc ggccaggcgc gtgacgaagt ttttctcgag
     1381 tcccgcggcg cgcttgccga acagatacga tgccagctgc aggacgcggc ccgcataggc
     1441 gatgatttgc accgtctcga tgccggagat gtcgtcgaag aaccagccgc agctcgtgta
     1501 catgagcatg gcgtggcgtt ccagctccat gagcatcagc gagcgcgtgc gctcctcttc
     1561 gttgaacgca tagagcgcat gctgctcgaa gaaggcgtcg cgcgaggcgc g
//
LOCUS       DASZYN010000082        22679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_68327, whole genome shotgun sequence.
ACCESSION   DASZYN010000082 DASZYN010000000
VERSION     DASZYN010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 22679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22679
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68327"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..244)
                     /locus_tag="VHE33_02135"
     CDS             complement(107..244)
                     /locus_tag="VHE33_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96276.1"
                     /translation="MRRTQNNPVTLSRKSWNESFVRAIAPRRYRRTLPTCTRPSLFGS
                     D"
     gene            351..845
                     /locus_tag="VHE33_02140"
     CDS             351..845
                     /locus_tag="VHE33_02140"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HVT96277.1"
                     /translation="MSIVRNAAAIAKGMSITFREIFQPTEVENYPDGPGPTRGAVLQE
                     RFRGAHVLQRDENGLEKCVACFLCAAACPSNCIYIEAAENTAEQRISSAERYAKVYNI
                     DYNRCIFCGYCVEACPTDAITHGHGFELASLNATNLVMRKEDMLVPGVGMPGQLAAQS
                     DSVK"
     gene            1034..4894
                     /gene="metH"
                     /locus_tag="VHE33_02145"
     CDS             1034..4894
                     /gene="metH"
                     /locus_tag="VHE33_02145"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HVT96278.1"
                     /translation="MTSKSQHPLRSILEQRIAIIDGAMGTTIRTYEMKEADIRGERFR
                     DANKDLLNNGDLFSLTQPAMIGDIHRRFLEAGADIIETNTFGATSIAQSEFFVDDPRE
                     HGGRKDPAFYQKIIDDKFLGDLAWEINEQSARQCREWADRIANATGRQRFVAGAIGPL
                     TVSLSNSPDADDPGFRVVTFDQVKTAYKQQVRALIAGGSDLLLVETIFDSLNAKAALV
                     AIREVFDEDGLTAKHQELPVMISAAVGRGGETMISAQTVPAFWDAVEHVQPFSVGLNC
                     SLGPDLMYPFLEELSQKADVAISAYPNAGLPNPLSPTGFDFGPGDMAKYLGDFARGGL
                     INIAGGCCGNTPEHIAAIAKALEGKEPRPLEPRVSGSQLQPPASVVEVETSAPAFVPL
                     RLSGSRPFTQQQNVFIMIGERTNVAGSPKFAKLIKEGKYEEAVSIARQQVENGANVID
                     ICMDEGMIDGVAAMSRFLQLLASEPEVAAVPFMIDSSKWEVIEAGLKCLQGKGIVNSI
                     SLKEGEAKFREHAAKVLKYGAAVVVMAFDEQGQAATFEDRIRVCERAYRILVDQVGFP
                     PEDIIFDPNILTVATGMEEHNNYAVDFIRAAKWIKENLPHAKVSGGVSNISFSFRGNN
                     KVREAMHAAFLYHAIGAGLDMGIVNAGQLEIYEEIEPELKILVEDVLLNRRPDATERL
                     VTFGEQLKAAGTDSTAVERQAEEWRHGTVEERLSHALVKGIDAYIDADTEEARQKLGR
                     PLAVIEGPLMAGMGVVGDLFGAGKMFLPQVVKSARVMKKAVAYLTPFMEAEKAALAAA
                     GHEVRAQGKIVLATVKGDVHDIGKNIVGVVLACNNYEVIDMGVMVPAEKILERAKAEK
                     ADIIGLSGLITPSLDEMVHVAREMERLDFKLPLLIGGATTSRAHTAIKIAPHYSEPVV
                     HVLDASRAVPVTTSLLSDESKAGFVTQLKVEYAAVRKAHATPKQPMVSLSAARARRTP
                     IDWKPEDIPTPSFTGVRVLDNFPLAELRRYIDWSPFFHTWELKGVYPRILDDEKQGAQ
                     ARQLFAEAQTLLDRIVDENLLTARAVYGLFPANAIGDDVELYADGARTTIQSRLHFLR
                     QQANREGSEPCRSLADFIAPKETGLKDTIGAFAVTSGGGLTDLVLKFKAQHDDYNAIM
                     AEALADRLAEAFAEYLHARVRKEWGYGCTEHLSHEDLIEEKYRGIRPAPGYPACPDHT
                     EKGTIWQLLDVEANAGIQLTESFAMWPGSSVSGLYFAHPEARYLTVGKIDRDQVADYA
                     ERKGMTVRDVERWLGPNLGYDPAE"
     gene            5246..5737
                     /locus_tag="VHE33_02150"
     CDS             5246..5737
                     /locus_tag="VHE33_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HVT96279.1"
                     /translation="MGAVHEQFLMKAAEAARSAGHLFPEFAACEAALESGWGLSHLAV
                     DANNLFGQKQAHPALAGTETVSLPTREYLHGAWVVVQANWVKFADWTACFRERMALLH
                     SLSAAYPNYKAALASTSGEQFLTEVSKTWSTDPGRAGKVLAIYDVHRKTLDGHSRASG
                     RSV"
     gene            complement(5860..10452)
                     /locus_tag="VHE33_02155"
     CDS             complement(5860..10452)
                     /locus_tag="VHE33_02155"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HVT96280.1"
                     /translation="MTGQVTMINEQRLRDAGLVPDQLRAAAREDMHRWGTVHGTRDLG
                     SFDQRVEAARQRLETLFQDLDSLPAQGWPGPEPLLEIRENPRMMRSVLDELRTVRRKL
                     RRLPHAVRDDHQDEPRVITVASAYLAASGSVWNADALRIYLEELQRAEALLLEELWVL
                     PVALRFCLLQAILDQATSRLEAFSLNDGTPHLEGETGDPAFAKLLTTRIQSLREIAYV
                     DWFFVMEPLVVFDAVLREDPAGAYPQMDFDSREVYRKEISRIARLSECTETEVARRAI
                     SLADAAKQQPVTDGRIYLRRAHVGYYLIDRGFEELKPKIGYHPRMIDRIRDGLRRYSD
                     DFYIGGIEVLTVILIAAVLLPLIPNYSIFGGLTFAFLLLLIPATQGAVDLVNNTISSV
                     FRPLPLPKIDLTDGIPADYTTMVAVPTLLLNDSQVRELVQALEIRCLANPDPNLHFAL
                     LTDLPDSVSRPRENDTDPLVVLAVRLINELNERYKDQKKFGRFLLLHRQRIFNARQGV
                     WMGWERKRGKLLDLNNLLTGHFDAFPVKAGDTSVLNKVRYIITLDSDTQLPRGTAHGM
                     IGAMMHPLNRAIIDPEYRVVKEGYGILQPRVGVSVHSAAQSRMANIYSGQTGFDIYTR
                     AISDVYQDLYGEGSFTGKGIYEVATLHTVLEKRFPRNALLSHDLIEGAYARAGLATDI
                     EVIDDYPSHYSAYTRRKHRWVRGDWQIVQWLFTRVPDETGHYVRNPISTISRWKILDN
                     LRRSLVEPATMILLVAGWLGLPGGGIYWTLVTLFLLFVPVFVQLMFGLGRSMFSEQEG
                     AAQDVVGGFQQSLVITLLTLAFLPHQTMMAIDAIIRAMVRRMITGQRLLEWETAAEAE
                     SSQRRSSTVDRYLAFTPLIAIVIGGVVAALHPVSLIPAVPILLLWGFENDITRWLNRP
                     PREPRQQLREEDDLFLREYSLRVWRFFHEFGGERHNYLIPDNVEEDELYEAARVSPTN
                     LGLLFNARQAACEFGFLTAPEFADLMQRSYATMAKLPLHRGHLYNWYTTDTLEALPPI
                     TVSSVDSGNFVASLYTLQMGTLGLLKRPLLDSRLFSGLATHLRLLKSLKNLPAPLSEL
                     HVPSDKATPDEWLAWLLAPETLEAFEKLQPYTRPSLDETLYWTNEVRSRIGAVATLVR
                     DFSPWRNPVYSSIRGVINERLAGDTAFRAFATDPDAPLMPPLEEAPGLAKALAQRLGA
                     PHPMPESAETAALIERLRTEVSAAAQRLQALVDSLRFVSREAERIADATDFAFLVNKD
                     RMLLSIGYEFSTGKIHWACYDMLASEARIATYIAVARGELSQQGWFKMSRVHTQAYGC
                     SVLLSWTGTMFEYLMPALWMRSYPDTLLSKSLLGSVEIQRSFAKANNLPYWGISESGY
                     AARTDQGHYHYQAYGIPQIALKWDATAGPVISPYSTFLALNVDPAASIRNLRRMASDG
                     YTGAYGFYEAIDFTGNKPEAVREWMAHHQGMCILGLLNLLEDNAVQRWFYDNTQMRSV
                     ELLLHEKPMREVKSKSAPAPSVSKKAKKPAALKKAG"
     gene            complement(10473..12296)
                     /locus_tag="VHE33_02160"
     CDS             complement(10473..12296)
                     /locus_tag="VHE33_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT96281.1"
                     /translation="MTNKSNSATHSAWGDRIRALRNVPPVLRILWESGPAVVTWGLIL
                     RVIVAAMPWAIGWVAHLIINGVDLALHRQPLPVHYWWIVASEVALALIFSAFTRAIDY
                     TDSLLADRYTHHVSIEVMRQAARLDLTTYEDPDFYDRLERARVQATDRLAMIQQMGRL
                     FLQVLTTLIWSAQLVYYSPWLMLLLIIGVLPSFFGETHFAFLGYAKNFRQTPIKRVMD
                     YLRLVGGSKEAAKELKLFNLSDYLTNRFAKLSEQIYHENVNLSRRKLLWGGLLSLLGT
                     CGYYGAYVYAIWEALHGKYNVGVFYLITTAIQQASGQLQQAFSTASGIADQALFLTDL
                     IGFFELKPVVQSKPNALLAPRPIRRGFEFRNVSFAYPGTERRVLKDFNFTIEPGQRIA
                     LIGENGQGKTTVVKLITRLYDPTEGQILLDGIDLREYDIDDLHREMGVIFQDFMRYEM
                     TVKENIGVGHIEVRHDDEDIRAAAEKSLAAEVVKKLAGGYEQMLGRRFVSGVDLSGGE
                     WQRIALARAYLRDAQLLILDEPTAALDAKSELEVFERFAELTAGKMALLISHRFSTVR
                     MADRIVVLSAGRLVEEGTHQQLMALGGQYAAMFEMQAASYR"
     gene            12765..14234
                     /locus_tag="VHE33_02165"
     CDS             12765..14234
                     /locus_tag="VHE33_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVT96282.1"
                     /translation="MSPEPSSSIAPPDHQLPPTTDPPKRRRHRWIWAVVLLAFGLLFY
                     WVFSYSKKNQAAVAGMGGGGRRGVMPGMSIPVVPATSKTGSLGVYLEAIGTVTPVHTV
                     SLTAQVTGVITAVHYREGQYVKKGDPLIDIDSRTYAAQLAQAQGTLERDQNMLAEAQM
                     DLARYQTAWSKNAIPRQTLEDQEKQVLQDQGTVKNDEGNVQYATVQLGYCHIVSPIDG
                     RVGLRLVDPGNLVTANSTTALVVVTQEAPITVIFTVAEDNLDQVLQQTRHGKQLSVDA
                     WNRDRSTKLASGKLMTVDNQIDTTTGTVKLRAQFPNSNGALFPNQFVNTRLLVKTLDN
                     QIMVPASAIQHNGDVAFVYLIKPGPGNPNASSAQAGPNQQASGARRGGTPQQGASGRH
                     AGGGGSGAGGPSGGMEQDAGPGRRGNQGAERAKYHVEMKEVKTGITDNDWTAVEGIPS
                     GTMVANSSFDKLQDQSDVSLSNAKIPGSPSTISSESDAP"
     gene            14231..17605
                     /locus_tag="VHE33_02170"
     CDS             14231..17605
                     /locus_tag="VHE33_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVT96283.1"
                     /translation="MSPSRPFILRPVATSLLMAAILLVGIVAYTQLPVSALPEVDYPT
                     IQVLTFYPGASPDVTATTITAPLERQFGEMQGLSQMTSTSAGGVSVIVLQFSLSMSID
                     VAEQEVQAAINGGGSFLPSNLPAPPVYNKTNPADAPILTMAITSDSMPLSQVEDLVDT
                     RLAPKIAQINGVGLVSISGGQKPAVRIQANPVKLAAYGLNMEDLRTALVSASVNSAKG
                     NFDGPRQDYQIDSNDQITEASQYNDVVVAWRNGAPVFVHNVAAVVNGVENTKQAAWMD
                     KTPAIILNIQRQPGANTITVVRSIQKILPQLETNLPASIRVQTLTDMTTSIQASVDDV
                     EFELVLTIGLVVLVIFLFLRSLYATIIPSVAVPLSLIGTFAVMYILGYSLDNLSLMAL
                     TISTGFVVDDAIVMIENISRYLEEGMAPMQAALTGAEQIGFTIVSLTVSLIAVLIPLL
                     FMGDVVGRLFREFAVTLAVTIVISAVVSLTLTPMMCSRILRHDPAEQHSRFYRTSERV
                     FNGMIAFYGRTLRVVLRYQTITLLVALGTLVLTVFLYIVIPKGFFPVQDTGVIQGITQ
                     APQDISFDAMAQKQQQVAAIILRDPAVESIASFIGTDGINTTLNSGRININLKPLDQR
                     SPSGTSASNVIRRLQNKLGGVSGMHLYMQPVQDITVDDRVSRTQYQYTLEDPDVNELN
                     EWTNKFVERLKKIDVLEDVATDQQTGGRAVSLMIDRVTASRLGIAPTTIDNTLYDAYG
                     QRLISTLYTQLNQYHIVLETAPQWAQDPANLGDLYIQGNASAGASGAGAVTSFSSSAS
                     ASAGSNALTTGTKYTPSSSVLTAPSRAVGGVSSLQGASSINGGSVAGTTPNAITSSAG
                     SHAIPLSAFTQATTTTEALSINHQGQFPAVTVSFNLAPDASLGTAITDIDKAADQLHF
                     PASLQASFQGTAAAFKNSLSNEALLILAALVTVYIVLGVLYESFIHPITILSTLPSAG
                     VGALLALMLFRQDLSVVAIIGIILLIGIVKKNGIMIVDFALEAEREQGKDSTEAIFEA
                     SMLRFRPIMMTTMAALLGGLPLALGRGLGSELRRPLGIAMVGGLLLSQILTLYTTPVI
                     YIFFDRLGQRISGRRPSVRAAEHTGPSVPTAEERHL"
     gene            17602..20739
                     /locus_tag="VHE33_02175"
     CDS             17602..20739
                     /locus_tag="VHE33_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVT96284.1"
                     /translation="MSFSWAFIKRPVGTTLLTIAVAIAGAIGFMVLPVAPLPQVDFPT
                     INVSASLPGASAAIMAASVATPLERQLGHIAGITEMTSSSSLGSTRITMQFDLSRNID
                     GAARDVQAAINAARTYLPANLPSNPRFFKSNPADAPIMILGLTSDKYDVTKVYDLAST
                     VLEQKLSTIDGVGQVFVGGGATPSVRVEVDPTKLESYGLTLSTVRSTLSLQNSHSPRG
                     QLSDGAVTADIITNDQISKADDYKPLVIGYHNGAAVRLSDVAAVNDSTENLRTAGYMD
                     GTRAVFIILFRQPGANIIQTVDNIKAQLPFLTAVLPHGINITTVLDRTTTIRASVATV
                     EKTLIGSIVLVVLVVFIFLRSPRATLIPSVAVPVSLIGTFAVMYLLGFSLDNLSLMAL
                     TIATGFVVDDAIVVMENITRHLESGMPPFNAALVGAREIGFTVFSISMSLIAVFIPIL
                     MMGGIIGRLFREFAITLSVAIVVSMVVSLTTTPMMCAHLLKHREEGEKHNIFYRASER
                     FFDGLLSIYRGSLRWALDNPVLILIVLGLTIALNLVVIVRIPKGFFPQQDTGAVVGGV
                     QGPQDASFAFMNYSIQQLVKVIRTNPNIAHVNAYTGGNGSSNGGFIYIALKPLGSGPG
                     ERTESAMQVIGDLRPKMARLPGASAFLQPVQDLRIGGRGSNSLYQYTIQSDDIPDLAK
                     WGPILLENMRKLPGLQDVNSDQQNGGLEQMLDYDRVTAARLGQTPQSLDSALYSAFGQ
                     SQVSVIYTQLNQYYVVMEVAPQYWQSPEGLKDIWLNTGSSGGAGINDMSPLATMTRVR
                     VNTTPLQVSHTGLFPSVTVSFNLAPGMSLSDAIREVTQMEERLGMPSTVHGLFAGTAQ
                     AYQASLATEKYLVITAILAVYIVLGILYESLVHPFTILSTLPSASIGAMLALMIFDID
                     LNVISIIGIILLIGIVKKNAIMMIDFALMAEREEQKSTRDAIFEACMLRFRPIMMTTM
                     AAICGALPLAFGTGTGSELRRPLGITIVGGLLLSQLVTLYTTPVVYLTLDALRLRIMG
                     RTHDTFRPEIEGAAS"
     gene            20754..22280
                     /locus_tag="VHE33_02180"
     CDS             20754..22280
                     /locus_tag="VHE33_02180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HVT96285.1"
                     /translation="MSGIVRNRGALIAFASAALALVTLAGCRVGPKYHPPAPPRATAP
                     NYKESTVNFQDEPGWKVASPQDAMLRGNWWEIFHEPELNALEDQLNINNQNIRQFFEQ
                     YMAARAVVAEARSQFWPTITLGPSFSRQKSSGNLRSSSVANTGTTSSTWAIPVDATWA
                     PDIWGKIRNQVRTAEYAAQISAADLENEKLTEQASLAQYYFEIRGQDMLQQILNQTVE
                     ADQKALDATRGSYDAGVGDYISVVEARNTLLSAQSSATNAGLLRAQYQHAIAMLLGKV
                     ATDFTIPVRPMNYAPPAIPTGVPSQLLERRPDVAAAERTLAEANATIGIGYGAFFPQI
                     NLSATGGTESSNIARLADWPSRFWSIGINAAQVLFDAGLYRAALHQYEAQYNADVASY
                     RQTSLTAFQQVEDALAATRIYSQQILHEEEAVKSAQEFLDLETQRYQLGVDPYVDVVT
                     AQTTLLGDRQTLNALHISQMIATVQLVQALGGGWDRSQLPSPEQVGAKAPNSAYTIEK
                     "
BASE COUNT         4618 a         7141 c         6556 g         4364 t
ORIGIN      
        1 gcggtggtcg agccatcggc aaacggcggg agaacgatgg cgatgcgggt gagagaggtc
       61 atcttcggac tcgacgctgg tgcgccagcg ttgagggcag cggtgactag tcgctgccga
      121 agagactcgg tcgcgtgcag gtcggcaggg tccgccggta gcggcgggga gcgatggcgc
      181 gcacaaacga ctcgttccag ctcttgcggc tgagagtcac cgggttgttc tgggtgcggc
      241 gcatcgggtt gcctggcctg agaatagcac gggtcacgcg cgattcgaca gcattaccaa
      301 ggggttggat ctgcccggcc ccgggcctca atcatccgta tactaagggt atgtcgattg
      361 ttcgcaacgc tgccgcgatc gccaaaggga tgagcatcac ctttcgcgag attttccagc
      421 ctaccgaggt cgagaactac cccgatggcc ctggccccac gcgcggagca gtgctgcagg
      481 agcgcttccg tggtgcgcat gtcctgcagc gcgacgaaaa tggcctcgaa aagtgcgtcg
      541 cctgcttcct gtgcgccgcc gcctgccctt ccaactgtat ctatattgaa gcggctgaga
      601 acaccgccga gcagcgcatc tccagcgccg aacgctatgc aaaggtctac aacatcgact
      661 acaaccgctg catcttctgc ggctattgcg tggaagcctg cccgaccgac gccattaccc
      721 acggccacgg ctttgaactc gccagcctga acgcgaccaa cctcgtcatg cgcaaggaag
      781 atatgctggt gcccggagtg ggcatgcccg gccagctggc ggcacagtcg gattcggtga
      841 aatagccccg aaaacttgcg cggccgaggc tttcctcacc gttcgactgc ggatttgacc
      901 tcctgatccc cgcaaacccc cgctcaccag cgtccggttc tggaatccgc tgcccgtccg
      961 ataacatcaa aggagtcaac gccaatctcg tgtccgatcc ggcgttcgcc gtgtcccagg
     1021 cggcaccaaa tacatgacct ccaagagcca gcatccgctt cgcagcattc tcgaacagcg
     1081 catcgccatc attgacggcg cgatgggcac cacgattcgc acctacgaga tgaaggaagc
     1141 cgacattcgc ggcgaacgct ttcgcgacgc gaacaaagac ctgctgaaca acggcgacct
     1201 cttctcgctt acccagcctg cgatgatcgg cgacatccac cgccgcttcc tcgaagccgg
     1261 tgccgacatc attgaaacca acacattcgg cgccaccagc atcgcccaga gcgagttctt
     1321 cgttgacgat ccgcgcgagc acggcggccg taaagatccg gctttctacc aaaaaatcat
     1381 cgacgacaaa ttccttggcg acctcgcgtg ggaaatcaac gagcaatcgg cgcgccagtg
     1441 ccgcgaatgg gccgaccgca ttgcaaacgc aaccggacgc cagcgcttcg ttgccggagc
     1501 catcgggccg ctcaccgtct cactttcgaa ctcgcccgat gccgacgatc cgggatttcg
     1561 cgtcgtcacc ttcgatcagg tgaagaccgc gtacaagcaa caggtgcgcg cgctcatcgc
     1621 cggcggatcg gatctgctgc tcgtcgagac cattttcgac tccctcaacg ccaaggccgc
     1681 gctcgtcgcc atccgcgagg tcttcgacga agacggtctc accgcgaagc accaggaact
     1741 ccccgtgatg atttcggccg ccgtcggccg tggtggcgag accatgatct ctgcgcagac
     1801 cgtcccagcc ttctgggacg cggtggagca cgtgcagccc ttctctgtag gcttgaactg
     1861 ctcgctcggc ccggacctca tgtatccgtt cctcgaagag ctatcgcaga aggccgacgt
     1921 cgccatctcc gcctacccaa atgccggctt gcccaacccg ctctcgccaa ccggattcga
     1981 ttttggcccg ggcgacatgg cgaagtacct gggcgacttc gcgcgcggcg gtctcatcaa
     2041 tattgccggc ggctgctgcg gcaacacgcc cgaacacatc gcggcgattg caaaagccct
     2101 ggaagggaag gagccgcgcc cgcttgaacc ccgcgtatcc ggctcccagc tgcagcctcc
     2161 ggcttcagtc gtcgaagtgg aaacgagcgc tccggccttc gttcccctcc gcctctccgg
     2221 ctcccgcccg tttacccagc aacagaacgt cttcatcatg attggcgagc gcaccaacgt
     2281 cgccggctcg ccaaaattcg caaagctcat caaagaaggg aagtacgaag aagcagtctc
     2341 catcgcccga cagcaggtag aaaatggcgc caacgtcatc gatatctgca tggacgaagg
     2401 catgatcgac ggggtcgccg ccatgtcgcg cttcctgcag ctgctggcca gcgagcccga
     2461 agttgctgcc gtccccttca tgatcgactc ctcgaaatgg gaggtgatcg aagccggact
     2521 gaagtgcctg cagggcaaag gcatcgtcaa ttccatttcc ctcaaggagg gcgaggcgaa
     2581 gtttcgcgaa cacgccgcga aggtgctgaa atacggcgcg gcggtcgtgg tcatggcctt
     2641 cgacgagcag ggccaggccg cgacctttga agatcgcatc cgcgtctgcg agcgggcgta
     2701 tcgcatcctc gttgaccagg tcggcttccc gcccgaggac atcatcttcg accccaacat
     2761 ccttactgtc gccaccggca tggaggagca caacaactac gcggtcgact ttatccgcgc
     2821 ggccaagtgg atcaaggaga atctgcccca cgcgaaagtc agcggcggcg tctcgaacat
     2881 ctccttcagc tttcgcggta acaacaaagt gcgcgaggcg atgcacgcgg cgtttctcta
     2941 tcacgccatc ggcgccggtc tcgacatggg catcgtcaat gccggacagc tcgagatcta
     3001 tgaggagatc gagcccgagc tgaagatcct cgtcgaagat gtgctgctca accgccgtcc
     3061 cgatgccacg gagcgattgg tcaccttcgg cgagcagctc aaagcggcag gcacggactc
     3121 gacggccgtc gaaaggcaag ccgaggaatg gcggcacggc accgtcgaag aacggctttc
     3181 gcacgcgctg gtcaagggca tcgacgctta tattgatgcg gataccgaag aggcccgaca
     3241 gaagctcggc cggcctctcg ccgtcatcga aggaccgctg atggcaggaa tgggcgtggt
     3301 cggcgacctg ttcggcgccg gcaaaatgtt cctgccccag gtggtcaaat ccgcccgcgt
     3361 tatgaaaaaa gccgtggctt acctcactcc gttcatggaa gcggagaaag cagcgcttgc
     3421 ggcagcgggc cacgaggtca gagcgcaggg aaaaattgtc ctcgccaccg tgaaaggcga
     3481 cgtacacgac atcggcaaga atatcgtggg cgtcgtgctt gcctgcaata actacgaagt
     3541 gatcgacatg ggcgtgatgg ttccggccga aaagattctc gagcgcgcca aagcggagaa
     3601 ggccgacatc atcgggctca gtggcctcat cacgccatcg ctggacgaga tggtccacgt
     3661 cgcccgcgag atggaacgtc tcgacttcaa gctgccgctg ttgatcggcg gtgccacgac
     3721 cagccgcgcg cacacggcta tcaaaattgc accccattac agcgaacccg tggttcacgt
     3781 gctcgacgcc agccgcgccg tgcccgtcac caccagcctg ctgagcgatg agagtaaggc
     3841 cggcttcgtt acacagctga aggtggaata tgccgcagtc cggaaggcgc atgccacgcc
     3901 gaagcagccg atggtttcac tctccgcggc gcgtgcgcgt cggaccccga tcgattggaa
     3961 acccgaagac atcccgacgc cctcattcac cggcgtgcgg gttcttgata acttcccgct
     4021 ggcggaactg cgcagataca tcgactggag cccgttcttt catacctggg agctgaaggg
     4081 cgtttatccg cgcattctcg acgacgaaaa gcagggtgct caggctcgtc aactcttcgc
     4141 ggaggcgcaa acacttctcg accgcatcgt tgacgagaac ctcctcacgg ctcgggccgt
     4201 ctatggactt tttcctgcca acgccattgg cgatgatgtc gaactctacg cggacggggc
     4261 gcgcaccacg atccagagcc gtctccattt cctgcgccaa caggccaatc gtgaaggcag
     4321 cgagccatgc cggtcgctgg cggattttat cgcgcccaaa gaaacgggtc taaaggacac
     4381 tattggcgcc ttcgcggtca ccagcggcgg cggcctgacg gacctcgtcc tgaagttcaa
     4441 ggctcagcac gacgattaca acgcgatcat ggcggaggcg cttgccgatc gcctggccga
     4501 ggcgtttgcc gagtatctgc acgcccgggt gcggaaagag tggggctacg gatgcacgga
     4561 acacctgtcc catgaggatt taatcgagga gaagtaccgc ggtatccgtc cagcgcccgg
     4621 ctatcccgcc tgccctgacc atacagagaa ggggacgatc tggcagcttc tcgatgtcga
     4681 agccaacgcc ggcatccagc ttaccgaatc gtttgcgatg tggccgggat cgagcgtcag
     4741 cggcctctac tttgcacatc cggaggcgcg ctatcttacg gttggcaaga ttgatcgcga
     4801 ccaggtcgcc gactacgccg aacgcaaggg catgactgtg cgcgacgtcg agcgctggct
     4861 cggcccgaat ctgggctacg atccagccga ataagctcgc ctcaaatcaa tgtccccccg
     4921 cccggcgaac agccaggttc accccacagc aagaagcaaa gacctttccc ggctccgttt
     4981 caaccgattt cgctctgccc cgacgccgga ggtaacgctt ttcccgattt cctcgcccgt
     5041 tttgaaacta ccttcgcctg cattgctcct tctcgcgccg gttcggtgtt tctatccttt
     5101 cgtcacgtga ccttcgttag ctgacgcctc agatttcact tgaacctgta gctccgcggc
     5161 accgacaata gaatctggaa gaaatagcag gcggatgcca gtcatggctt ccgccttttt
     5221 tattgcacgc gaaaggaaag aaacaatggg agcggttcac gaacaatttt tgatgaaggc
     5281 ggccgaggca gcacggtccg cgggacatct tttcccggaa tttgcggcct gcgaggcagc
     5341 tctcgagtcc ggctggggct tgtcgcatct cgccgtggat gcgaacaatt tgttcgggca
     5401 gaagcaggcg catccggcgc tggccggaac agagacagtg tcgctgccga ctcgtgaata
     5461 tctgcacggg gcctgggttg tcgtccaggc caattgggtg aagttcgcgg actggacggc
     5521 ttgctttcgg gagcgcatgg cgctcctgca ctcgctcagc gcagcttatc ccaactacaa
     5581 ggcagcactg gcgtccacct cgggcgagca gttcctcacg gaggtgtcga agacctggtc
     5641 cacggatccc ggccgtgcgg gcaaggttct ggccatatac gacgtccacc ggaagacgct
     5701 ggacgggcat tcgcgggcca gcgggcgatc cgtttgagca cccagtcgcg gtataggcag
     5761 gacccgcaaa gcgggaccgg cttatgttgt caagtttgta atcggccgct aaatgctgtt
     5821 ttttcagtta gcagcgggca ggcagaaaag aggaaatgac tacccggcct tcttcagcgc
     5881 tgccggcttc tttgccttct tcgataccga aggagccggc gccgacttcg acttgacctc
     5941 gcgcatcggc ttttcgtgga gcaacaactc aaccgaacgc atctgcgtgt tgtcgtaaaa
     6001 ccagcgctgc actgcgttgt cttcgagcag gttcagcagg cccaggatgc acatgccctg
     6061 gtggtgcgcc atccattcgc gcaccgcttc gggcttgttg ccggtgaagt cgatggcctc
     6121 gtaaaagcca taggcccccg tatacccatc ggatgccatg cgccgcaagt tacggatcga
     6181 tgccgccgga tcgacgttca gcgccagaaa ggtcgaatac ggagaaatga ccggacccgc
     6241 cgtcgcgtcc cacttcagcg cgatctgcgg gatcccgtac gcctggtagt ggtagtgtcc
     6301 ctggtcggtc ctggccgcgt agcccgattc cgagatcccc cagtagggca ggttgttggc
     6361 cttagcgaac gagcgctgaa tctccaccga tcccagcagc gatttcgaca gcagagtgtc
     6421 aggatagctc cgcatccaca gcgccggcat caggtattcg aacatcgttc ccgtccagga
     6481 cagcagtacg gagcagccgt aagcctgggt gtgcacgcgc gacatcttaa accagccttg
     6541 ctgcgaaagc tctccgcgtg ccaccgcaat atacgtcgcg atccgcgctt ctgacgccag
     6601 catgtcgtag caggcccagt gaatcttgcc ggtcgaaaac tcgtagccaa tcgagagcaa
     6661 catccggtcc ttgttcacca gaaaagcgaa atcggttgcg tcggcgatgc gctccgcttc
     6721 cctggagaca aagcgcagac tgtctaccag cgcctgaaga cgctgcgccg cagcggagac
     6781 ctcggtcctg agccgctcga tgagcgccgc agtctcggcc gattccggca tcggatgggg
     6841 cgcgcccagg cgctgcgcca gcgctttagc cagtccagga gcttcttcca gcggcggcat
     6901 cagcggcgcg tcaggatcgg tcgcaaaagc gcggaaagca gtgtcaccag ccagacgttc
     6961 gttgatgaca ccgcgaatcg acgaatagac cggattcctc catggcgaga aatcacggac
     7021 cagcgtcgcg accgccccga ttcggctgcg cacctcattg gtccagtaaa gtgtctcgtc
     7081 cagcgaaggc cgggtatacg gctgtagctt ctcgaacgcc tccaacgtct ccggtgcgag
     7141 aagccaggcg agccactcgt ccggtgtggc cttgtccgac ggaacgtgaa gctcagacag
     7201 cggtgcgggc aggttcttca gcgacttcag caggcgcagg tgcgttgcaa gcccggaaaa
     7261 cagtcgcgaa tcgagcaacg ggcgtttgag cagccccagg gtgcccatct gcagcgtgta
     7321 gagcgatgca acaaagttgc cgctgtcgac cgaagagacc gtaatcggcg gtagcgcctc
     7381 cagcgtatcc gtggtgtacc agttatagag atggccgcga tgaagcggca gcttcgccat
     7441 cgtcgcatag ctgcgctgca tcagatcggc gaattccggt gcggtgagaa acccaaattc
     7501 acaggccgcc tggcgcgcat tgaacagcaa cccgagattt gtgggtgaga cacgtgccgc
     7561 ttcgtaaagc tcgtcttcct cgacattgtc ggggatcagg tagttgtgac gctcgccgcc
     7621 aaattcatgg aagaatcgcc aaacgcgcag ggagtactcg cgcagaaaca gatcgtcttc
     7681 ctcgcgcaat tgttggcgag gttcacgcgg tggccgattg agccagcgag taatgtcatt
     7741 ctcaaagccc cagagcaaca ggattggcac agccgggatg agagaaaccg gatggagagc
     7801 agcgactacg cctccaataa cgatcgcgat caacggtgtg aatgccagat agcgatccac
     7861 ggtcgaagag cggcgctgcg acgactccgc ttcggcggct gtttcccact caagcaggcg
     7921 ctgtccggtg atcatgcggc ggaccatggc gcgaatgatg gcgtcgatgg ccatcatggt
     7981 ctggtggggc agaaaggcga gggtcagcaa ggtgatgacc agcgactgct ggaatccgcc
     8041 gacaacgtcc tgagccgcgc cctcctgttc gctgaacatg gaacggccca ggccgaacat
     8101 gagctgtacg aagacgggaa cgaacagcag gaacagcgtg accagcgtcc agtagattcc
     8161 gcctcccggc agaccgagcc agccggcaac cagcaggatc atggttgccg gttcgaccag
     8221 ggagcggcgc agattgtcga ggatcttcca gcgagagatg gttgagatcg gattgcgcac
     8281 gtagtggccg gtctcgtccg gcaccctcgt gaacagccac tgcacgatct gccagtcgcc
     8341 gcgtacccag cgatgtttgc gacgggtgta ggcgctgtag tgagaaggat agtcatcaat
     8401 gacctcgata tcggtagcca gaccggcgcg tgcgtaggct ccctcgatca ggtcgtgact
     8461 tagcagggca ttgcgaggaa agcgcttctc cagcacggta tgcagggtcg cgacttcata
     8521 aatccctttg ccggtgaagc tgccctcacc gtaaagatcc tgataaacgt cagaaatggc
     8581 gcgtgtgtaa atatcgaagc cggtctggcc ggagtagatg ttggccatgc gcgactgcgc
     8641 cgccgaatgg acactcacgc cgacgcgggg ctgcagaatg ccgtagccct ctttgacgac
     8701 ccgatattcg ggatcgatga tcgccctgtt cagcggatgc atcatcgcgc cgatcatgcc
     8761 atgtgctgtg ccgcggggta attgcgtatc ggagtcaagc gtgatgatgt agcgcacctt
     8821 gttgagcacg ctggtatcgc cggcctttac cggaaaggcg tcgaaatggc cggtgagcag
     8881 gttgttcagg tcgagcagct tgccgcgctt gcgctcccaa cccatccaaa cgccctggcg
     8941 cgcgttgaag atgcgctggc gatgaagcaa caggaaacgg ccgaactttt tctgatcctt
     9001 ataccgttcg ttcagttcgt tgatcaggcg aacagccagc acaaccagcg ggtccgtgtc
     9061 gttctcgcgc gggcggctga ccgaatcagg cagatctgtc agcagcgcga agtggagatt
     9121 gggatcagga ttggccagac aacggatctc aagtgcctgg accagttcac gcacctgact
     9181 gtcattcagc agcagagtcg gcaccgccac catggtggtg tagtccgcgg gaatgccgtc
     9241 ggtcagatca atcttgggca atggcagcgg ccgaaagacg gatgagatgg tgttgttgac
     9301 caaatccacg gcgccctggg ttgcagggat gagcaacaga aggaaagcaa atgtgaggcc
     9361 gccaaagatc gagtagttgg ggattaacgg gagcagcacc gccgcgatca gaatgaccgt
     9421 gagaacctca atgccgccga tgtagaaatc gtccgagtaa cggcgcaatc catcgcgaat
     9481 gcggtcgatc atacgcgggt ggtagccgat tttcggcttg agctcttcaa acccgcggtc
     9541 gatcaggtag tagccaacgt gcgcgcgacg caggtagata cgcccatcgg taacgggctg
     9601 ctgtttggca gcgtcggcaa ggctgatcgc gcggcgtgca acctcggtct ctgtgcactc
     9661 agagagacga gcgatgcgcg agatctcttt gcggtagact tcgcggctgt cgaagtccat
     9721 ctgcggatac gccccggctg gatcctcgcg caggactgcg tcgaagacga ccagcggttc
     9781 catgacgaag aaccagtcga catacgcgat ctcacgtaac gactggatgc gggtggtcag
     9841 cagcttcgca aacgctgggt cgccggtctc gccctcaaga tgcggtgtgc catcgttgag
     9901 ggagaaagcc tcgaggcgcg acgtcgcctg atcgaggatg gcctgaagaa ggcagaagcg
     9961 cagcgccacc ggcaaaaccc acaactcctc cagcaagaga gcttcagcgc gctgcagttc
    10021 ttccagataa atgcgaaggg catcggcgtt ccagacagag ccgctggccg caagatacgc
    10081 cgacgcgacg gtgattacgc gaggctcgtc ctgatgatcg tccctgactg cgtggggtaa
    10141 ccgccgcaat ttgcggcgaa cggtgcgcag ctcgtccagc accgaacgca tcatgcgtgg
    10201 attctcgcga atctccagta atggttcagg cccaggccag ccttgtgcgg gcagtgaatc
    10261 caggtcctga aagagcgttt caagccgttg acgggcggcc tccactctct gatcgaaaga
    10321 gccaagatct ctggtgccat ggacggtgcc ccagcgatgc atatcttcgc gggcggcggc
    10381 gcgcagttga tccggtacca gacctgcgtc acgcaaacgc tgctcattga tcatcgtcac
    10441 ctgacccgtc atacacacta ctccactgag tgttagcgat agctcgctgc ctgcatctcg
    10501 aacattgccg cgtactggcc acccagtgcc atgagttgct ggtgggtgcc ttcttccaca
    10561 aggcggccgg ccgacagtac tacgatgcgg tctgccatgc gcaccgttga gaagcgatgc
    10621 gaaatcagga gtgccatttt ccccgccgtc aattcggcaa aacgctcaaa gacttccagc
    10681 tcgctcttcg catccagggc tgcagtcggc tcgtcgagga tcagcagttg cgcatcgcgc
    10741 agataggccc gcgccagggc gatgcgctgc cactcgccgc cggagagatc caccccgctc
    10801 acaaagcgcc gtccgagcat ctgctcgtag ccgccggcca gtttcttcac gacttccgcc
    10861 gccaggctct tttcagcggc agctcgaatg tcctcgtcgt cgtgtcgcac ttcgatatgc
    10921 cccacgccaa tgttttcctt cacggtcatt tcgtatcgca tgaagtcctg gaagatgacg
    10981 cccatctcgc ggtgcaggtc gtcgatgtcg tattcgcgca ggtcaatgcc gtccagcaga
    11041 atctgtccct cggtcggatc gtagaggcgt gtgatcagct tgacgacggt ggttttaccc
    11101 tgaccgttct cgccgatgag tgcgatgcgc tgccccggct cgatcgtgaa gttgaagtcc
    11161 ttcagcaccc gccgttccgt acctggatag gcaaacgaca cattgcggaa ctcgaagccg
    11221 cgccgaatcg gacgcggagc gagcagagca ttcggcttcg actgcactac cggcttcagt
    11281 tcgaagaagc cgatcaggtc ggtcaggaag agggcctgat ccgcgattcc ggaagccgtg
    11341 gagaacgcct gctgcaactg accactagcc tgctgaatgg ccgtcgtaat caggtaaaac
    11401 acgccgacgt tatattttcc gtgcagcgct tcccagatcg cgtaaacgta ggcgccatag
    11461 tagccgcagg ttccgagcag cgaaaggagt ccgccccaca gtagcttgcg ccgggagagg
    11521 tttacgtttt cgtggtaaat ctgctcggag agtttagcga agcggttggt caggtagtcg
    11581 ctcaggttga agagcttcag ctctttggcg gcttccttgc tgccgcccac caggcgcagg
    11641 tagtccatca cgcgcttgat gggagtctgg cggaaatttt tggcgtagcc gagaaaggcg
    11701 aagtgggttt cgccgaagaa tgaaggcagc acgccgataa tcagcagtag catcagccac
    11761 ggcgagtagt agaccagctg cgcactccag atcagcgtcg taagcacctg cagaaagagc
    11821 cgacccatct gctggatcat ggccagccgg tcggtcgcct gcactcgggc gcgctccagc
    11881 cgatcgtaga agtcgggatc ttcgtaggtg gtcagatcca gacgggccgc ctggcgcatc
    11941 acctcaatgc tcacatggtg cgtatagcga tccgccagaa gcgagtcggt gtaatcgatc
    12001 gcgcgcgtga acgcactgaa gatcagagcc agagccactt cgctggccac gatccaccag
    12061 tagtggacag gcagcggttg acgatgcaga gccagatcga cgccgttgat gataaggtgt
    12121 gcgacccagc cgatggccca cggcatggcc gccacgatga cgcgcaggat taatccccat
    12181 gtaacaaccg cggggccgga ctcccacagt atccgcagga ccggcggcac gttacgcagg
    12241 gcgcgaatcc gatcgcccca cgcgctgtgg gtcgctgagt tacttttgtt ggtcatttgg
    12301 atcgcaagtc gaggtggaag aagcgcaatc gcggcagacg acgccggagt attggtttgg
    12361 atgcgggcgc atcaggcgta agttgttacc agtgctttcg atagtaccct ggcgcttgca
    12421 cccgcagccg atcatgagcc cagccgtcga gtgtttccgg cgaacactat atgaaggagg
    12481 gacccctctt tttcggcaac ggtttatcgg gcgaaattgc gacaaattcg ggcgttcatc
    12541 cgtccctata ttcgttgacg cgtaggaggg gaacacccat tttgcgccgg ttgccggcgg
    12601 cccaaacgcc gttccctgtt ctgaacgggc cttcgcaacg agtgttaaac ttcacatttg
    12661 gcgcatgttt tcgaatcggc gcctgttccc tccggcggct tcgctgctcc cctcggaaaa
    12721 cctgctttga acgacgaaga ccgatacaag accgacacaa atagatgagc cctgaacctt
    12781 cttcttccat tgccccgccg gaccatcaac tgcctccgac gacggatcct cccaaacggc
    12841 gacgccatcg ctggatctgg gcggtcgtcc tgctcgcctt tggcctgctt ttttactggg
    12901 tgttctccta cagcaagaaa aatcaggctg cggtcgccgg gatgggcggt ggcggacgcc
    12961 gcggtgtgat gccgggcatg tcgatcccgg ttgtgccggc gacatcgaaa acgggcagcc
    13021 tgggcgtcta cctggaagcc attggcacgg tcactccggt ccacacagta tccctgacgg
    13081 cgcaggtcac aggggtgatc accgcggtgc attatcgcga aggccagtac gtgaaaaagg
    13141 gcgatcctct tatcgacatc gattcgcgca cctatgcagc gcagctggct caggcgcagg
    13201 ggacgctgga acgggatcag aacatgctcg ccgaagccca aatggatctg gcgcgctatc
    13261 agacggcctg gtcgaagaac gccatcccgc ggcagacgct ggaggaccag gaaaagcagg
    13321 ttctgcagga tcagggtacg gtcaaaaatg acgaggggaa cgtgcagtac gccaccgtgc
    13381 agcttggcta ctgccacatt gtttccccga tcgacgggcg agtcggcctc cgcctggtcg
    13441 atcccggcaa tctggttaca gccaattcga caacagccct cgtggttgtg actcaggaag
    13501 cgcccattac ggtcatcttc accgtcgctg aagacaatct ggaccaggtc ctccaacaga
    13561 cacggcatgg aaaacagctg agtgtcgatg cctggaaccg cgaccgttcc acgaagctgg
    13621 ccagcggaaa gctgatgacg gtcgacaacc agatcgacac aaccaccgga acggtgaagc
    13681 tgcgcgcgca gttccccaac agcaatggag cgctgtttcc caatcagttt gtcaatacca
    13741 ggctgctggt taagacgctc gacaaccaga tcatggtgcc cgcgtcggcc attcaacata
    13801 acggcgacgt ggcgtttgta tatctgatca agcctggacc gggtaatccg aatgcgtcat
    13861 cggcacaggc cggcccaaac cagcaggcca gcggcgcaag gcggggcggc acaccgcaac
    13921 agggggccag tggccggcac gccggtggcg gtggcagcgg cgccgggggg cccagcggcg
    13981 gcatggagca ggatgcaggg cctggccgca gaggcaatca gggtgctgaa cgagcgaaat
    14041 atcacgttga aatgaaagag gtaaagaccg gtatcaccga caacgactgg accgcggtcg
    14101 agggcatccc ctcgggcacc atggtcgcga acagcagctt cgacaagctt caggaccagt
    14161 ccgacgtctc tctttccaac gccaagattc ccggatcgcc atcgacgatc tccagtgaga
    14221 gcgacgctcc gtgagtccgt cccgcccatt cattctgcgg ccggtagcca cctcgctgct
    14281 gatggctgcg attctgctgg tgggcatcgt tgcgtacaca cagctccctg tttcggccct
    14341 tcctgaggtc gactacccta ccatccaggt actcacgttc tatccgggcg ccagtccgga
    14401 cgtcaccgcc accacgatca cggcgcccct cgagcgtcag ttcggcgaga tgcagggtct
    14461 cagccagatg acttccacca gcgccggagg cgtctcggtc atcgtcctgc agttcagcct
    14521 ctcgatgtcc attgatgtgg ccgaacagga agtgcaggca gccatcaatg gagggggttc
    14581 attcctgccc tcgaacctgc ctgcgcctcc ggtctacaac aagacaaatc cggccgatgc
    14641 acccatcctg accatggcca tcacctcgga ttcgatgcct ctctcgcagg tcgaagacct
    14701 ggtcgataca cggctggcgc cgaagatcgc ccagatcaac ggcgtgggtc ttgtgagcat
    14761 cagcggcggt caaaagccgg ccgttcgcat ccaggcgaac cccgtcaagc tggcggccta
    14821 tggcctgaac atggaggatc tgcgcacggc gctcgtgagc gccagcgtta attcggccaa
    14881 gggcaacttc gacggtcccc gtcaggacta ccagatcgac tcgaacgatc agatcacgga
    14941 ggccagccag tataacgacg tcgtggtcgc gtggcgtaac ggcgctcccg tgttcgtgca
    15001 taacgttgcc gccgtcgtga acggcgtgga gaacacgaaa caggccgcct ggatggacaa
    15061 gacgccggcc atcatcctca acatccagcg acaaccaggc gccaatacca tcaccgtcgt
    15121 ccgcagtatt cagaagatcc tgcctcagtt ggaaacgaat ctgcccgcgt ccattcgggt
    15181 gcagacactc acggacatga cgaccagcat tcaggcctcg gtcgatgatg tcgagttcga
    15241 actggtgctc accatcggcc tggtggtgct ggtcatcttc cttttcctgc gcagcctcta
    15301 cgcgaccatt atccccagcg tggccgtgcc gctctcgcta atcggcacgt tcgccgtgat
    15361 gtacatcctc ggctacagcc tggacaactt atcgctgatg gcgctcacga tttcgactgg
    15421 tttcgtggtc gatgatgcca tcgtgatgat cgagaacatc tctcgatatc tcgaagaagg
    15481 gatggcgccc atgcaggcgg cgctgactgg cgccgagcag atcggcttca ctatcgtttc
    15541 tctgacggtc tcgctgatcg cagtgttgat cccgctgctc ttcatggggg acgtcgtggg
    15601 gcggcttttc cgcgaattcg cggtgaccct ggccgtcacg atcgtgatct ccgcagtcgt
    15661 atccctcacc ctgacgccga tgatgtgctc gcgcatcctg cgccacgatc cggccgaaca
    15721 gcactcgcgc ttctatcgca catccgagcg tgtgttcaac ggcatgatcg cattttacgg
    15781 acgcacgcta cgtgtggtcc tgcgctacca gaccatcacc ctgctggtgg ctctgggcac
    15841 gctggtgctt accgtcttcc tctatatcgt cattcctaaa ggcttcttcc cggtacagga
    15901 caccggcgta attcaaggca tcacgcaggc gccccaggat atttccttcg acgcgatggc
    15961 gcagaagcag cagcaggtcg ctgccatcat tctgcgcgat ccggccgtcg agagcattgc
    16021 ttcgttcatt ggtaccgacg gcatcaatac cacgctgaac agcgggcgga tcaacatcaa
    16081 cctcaagccc ctggatcagc gctcgccaag tggcaccagc gcgtccaacg tcattcgccg
    16141 cctgcagaat aagctcggtg gagtctccgg catgcatctc tacatgcagc cggtacagga
    16201 catcaccgtg gacgaccgcg tgagccgcac ccagtaccag tacacgctgg aagatccgga
    16261 cgtgaacgaa ctgaatgagt ggaccaacaa atttgtcgag cggttgaaga agatcgatgt
    16321 actggaagac gtagccaccg accagcaaac cggcggccgc gcggtttcgc tgatgatcga
    16381 ccgggttacc gcctcccgac tcggcatcgc ccccacaacg attgataaca cgctgtacga
    16441 tgcctacggc cagcgcctca tcagcacgct ttatacccag ctgaaccaat accacatcgt
    16501 gctggaaacc gcgccgcaat gggcacagga tccggctaat cttggcgatc tctacattca
    16561 gggcaacgcg tcggcgggcg caagtggagc cggcgcagtc acctcttttt catcttcggc
    16621 ctcggcctcg gctggctcca acgcgctcac cacgggcacg aaatacaccc cgtcgtccag
    16681 tgtcctgacc gcaccgtcgc gagccgtcgg cggcgtctca agtctccagg gcgcgtcctc
    16741 catcaacgga ggctccgtgg ccggcaccac gccaaacgct attacctcga gcgccggctc
    16801 gcacgccatt ccgctcagtg ccttcacgca ggccaccacc accaccgaag cgctgtcgat
    16861 caaccatcag gggcagtttc cggccgttac cgtgtccttc aatttggctc ccgatgcttc
    16921 cctcggcacg gcgatcaccg atatcgacaa ggcggccgat caactccatt ttccggccag
    16981 tctgcaggcc tccttccagg gcactgcggc cgcgtttaaa aattcacttt cgaacgaagc
    17041 cctgctgatc ctcgctgcgc tggttaccgt ctacatcgtt ctgggcgttc tctacgagag
    17101 cttcatccac cccatcacga ttctctctac gctgccttct gccggcgtcg gcgcattact
    17161 ggcgctcatg cttttccgcc aggacctcag cgttgttgcc atcatcggca tcatcctcct
    17221 catcggcatt gtgaagaaga acggcatcat gattgtcgat ttcgcgctcg aagccgagcg
    17281 cgaacagggc aaggattcaa ccgaagccat ctttgaagcc agcatgcttc gcttccggcc
    17341 catcatgatg accacgatgg cagccctcct tggaggacta cctctggcgc tcggtcgcgg
    17401 cctcggttcg gaactgcgca gaccgctggg catcgcgatg gtcggagggc tgctgctcag
    17461 ccagattctc accctctaca ccacgcccgt catctatatc tttttcgatc gacttggtca
    17521 gcgcatctcg gggcgtcggc cttctgtccg cgccgccgag cacaccggac cttcggtgcc
    17581 caccgctgag gagcgccacc tgtgagcttc tcctgggcat tcatcaagcg gccggtgggc
    17641 accacgctgc tcaccattgc cgtggccatt gcgggggcga ttggatttat ggtgctcccg
    17701 gtggcaccac tgccccaggt ggattttccc accatcaacg tgagcgccag cctgcccgga
    17761 gccagcgctg ccatcatggc tgcttccgtc gcaacaccgc tggaacgcca gctcggacac
    17821 atcgccggca tcacggagat gacttcatcg agcagcctcg gcagcactcg catcaccatg
    17881 cagttcgacc tgagccggaa catcgacggt gcggcacgcg acgtgcaggc ggcgatcaac
    17941 gctgctcgca cttacttgcc ggcgaatctg ccatccaatc ctcgcttctt caaatcgaat
    18001 cccgctgacg cgcccatcat gattctcggc ctgacgtcgg acaagtacga cgtgacgaag
    18061 gtctacgacc tggcatcgac ggttctcgag cagaagctgt caaccattga tggcgtcggc
    18121 caggtcttcg tcggaggcgg cgcaacaccg tccgttcgag tcgaagtcga cccaacgaag
    18181 ctggagagct acggcctgac cctctccacc gtgcgctcca cgctgagcct gcagaattcg
    18241 cacagccccc gcggccagct ttccgatggc gcggttaccg ccgatatcat caccaacgat
    18301 cagatttcaa aggcggatga ttacaagccg ctggtcatcg gctatcacaa cggcgcggcg
    18361 gttcgcctgt ccgatgttgc tgccgtgaac gactcgactg agaatcttcg taccgcgggg
    18421 tacatggatg gcacgcgtgc cgtgttcatc atcctgttcc gccagccagg cgccaacatc
    18481 attcagacag tcgacaacat caaagcgcag ctcccctttc tcaccgctgt tctgccgcat
    18541 ggcatcaaca tcacgactgt cctggaccga accaccacta tccgcgcttc ggtggcgacg
    18601 gtggaaaaga ccctcatcgg ctcaatcgtg ctggtcgtgc tggtcgtgtt catctttctg
    18661 cgcagcccgc gcgccacgct catccccagc gtcgccgttc ctgtttctct gatcgggacc
    18721 ttcgccgtga tgtacctgct cggcttcagt ctcgacaatc tctcgctgat ggcactcacc
    18781 atcgctaccg gtttcgtcgt cgacgacgcc atcgtagtca tggagaacat tacccgccat
    18841 ctggagagtg gcatgccgcc tttcaatgcc gcgctggtcg gtgctcgcga gattggcttt
    18901 acggtcttct ccatcagcat gtcgctgatc gctgtgttca tcccaattct catgatgggc
    18961 gggatcatcg gacggctctt ccgcgaattt gcgattacgc tctccgtcgc aatcgtcgtt
    19021 tcgatggtcg tctcgctgac caccactccg atgatgtgcg cccatctgct gaaacaccgg
    19081 gaagagggcg agaagcacaa catcttctac cgcgcgagcg aaagattctt tgacggcctg
    19141 ctttccatct atcgtggttc gctgcgctgg gcacttgata atccggtact catcctgatt
    19201 gtgctgggcc tgaccatcgc tctgaacctg gtggtcatcg ttcgcattcc caaaggattc
    19261 tttcctcagc aggacaccgg cgcggtcgtg ggcggcgtcc agggaccgca ggatgcctcc
    19321 tttgccttca tgaactactc gatccagcaa ttggtgaagg tcatccgcac caatcccaac
    19381 atcgcacacg taaatgccta caccggcggc aacggatcca gcaacggcgg cttcatttac
    19441 attgcactca agcccctggg cagtggtccg ggtgaacgta cggaaagcgc tatgcaggtc
    19501 atcggcgacc tccgccccaa aatggcccgt ctgccaggcg cctcagcctt cctgcagccc
    19561 gtccaggacc tccgtatcgg tggtcgcggc agcaactcgc tgtatcagta caccatccag
    19621 tccgatgaca ttcctgacct cgcgaagtgg ggccccatcc tgctcgagaa catgcgtaag
    19681 ctccccggcc tgcaggacgt gaactcggac cagcagaacg gcgggctcga acagatgctc
    19741 gactacgacc gggtcacggc cgcgcgcctc gggcaaacgc cgcaaagcct ggactctgca
    19801 ctctacagtg ccttcggaca gtcgcaggtc tccgttatct acacgcagct caaccagtat
    19861 tacgtggtca tggaggtcgc gccccagtac tggcaatcgc ccgagggatt gaaggacatc
    19921 tggctgaaca cgggcagttc cggcggcgca ggcattaacg acatgagtcc cctggccacc
    19981 atgacccggg tccgagtcaa caccacccca cttcaggtca gccacaccgg tttattccct
    20041 tcggtcaccg tctctttcaa ccttgccccc ggcatgtcgc tcagcgatgc cattcgtgag
    20101 gtcacccaga tggaagagcg gctgggaatg ccgtcgacgg ttcacggcct gttcgccgga
    20161 accgcccagg cctatcaggc ctcgctcgcg accgagaagt atctggtgat tacggctatc
    20221 ctcgcggtct atatcgtgct cggcattttg tacgagagcc tggtgcaccc gttcaccatt
    20281 ctctccacgc tgccctcggc cagcatcggc gcgatgctcg cattgatgat cttcgacatc
    20341 gacctgaacg tcatctccat catcggcatc atcctgctca tcggcattgt gaagaagaac
    20401 gctattatga tgatcgactt tgccctgatg gcggagcgcg aggagcagaa atccacgcgc
    20461 gacgccatct ttgaggcctg catgctgcgt ttccggccga tcatgatgac caccatggcc
    20521 gcgatctgcg gcgctctgcc gctcgctttc ggcacaggta ccggctcgga actgcgtcgc
    20581 cccctcggca tcacgattgt cggtggactc ctgctcagcc agctcgtgac cctctacacc
    20641 acgcccgtgg tctacctgac cctcgatgct ctgcgcctcc gcatcatggg caggacgcac
    20701 gatactttcc ggccggaaat agaaggagcc gcgtcgtgag ggccggaaga aagatgtctg
    20761 gaatcgtccg aaaccgtggc gcgctgatcg cgttcgcatc ggctgctctc gccctggtca
    20821 cgctggccgg ttgccgtgtt ggtccgaaat atcacccgcc tgcgccgccc agggccaccg
    20881 cacccaacta caaagaatcc acggtgaact tccaggatga gccgggctgg aaggttgcca
    20941 gcccgcaaga cgcgatgctg cgcggcaact ggtgggagat tttccatgaa ccggagctga
    21001 acgccctcga agaccagctc aatatcaata accagaacat caggcaattc ttcgagcagt
    21061 acatggccgc gcgcgcggtc gtcgccgagg cgcgctcgca gttctggccg accatcaccc
    21121 tcggaccctc tttcagccgc caaaagagct ccggcaatct gcgctcatcg agcgttgcca
    21181 acacgggaac caccagctcc acctgggcca ttcccgtcga cgccacctgg gcgccggata
    21241 tctgggggaa gattcgcaac caggtgcgca cggcggaata cgccgctcag atcagtgctg
    21301 ccgatctcga aaacgaaaag ctgactgagc aggcctccct ggcccagtac tatttcgaga
    21361 ttcgcggcca ggacatgctg cagcagattc tcaatcagac ggtcgaggcc gatcagaaag
    21421 cactggatgc gacccggggt tcctacgacg ccggcgtagg ggactacatt tcggtcgtcg
    21481 aagcccgcaa tacgctgctg agcgcccagt cttccgccac caacgcaggc ctgctccgcg
    21541 cccagtacca gcacgccatc gcgatgctgc ttggcaaagt cgccaccgac ttcaccattc
    21601 cagtgcgccc catgaactat gcgccgccgg ccattcccac gggtgtgccc tcccaattgc
    21661 ttgagcgccg gcccgacgta gctgcggctg agcgaaccct cgccgaggcg aatgccacca
    21721 tcggcatcgg ttatggcgcg ttctttccgc aaatcaacct gtcggccacc ggaggcactg
    21781 agtcctcgaa tatcgcacgt ttagcggact ggcccagccg cttctggtcg atcggtatca
    21841 atgcggccca ggtcctcttc gatgccgggc tctaccgagc ggctctgcac cagtacgagg
    21901 cgcagtacaa cgctgacgtc gccagctacc ggcagacctc cctgactgcg tttcagcagg
    21961 tcgaagatgc tctggcagcc acgcgcatct attcgcagca gatcctccac gaagaagaag
    22021 cggtgaagtc cgcccaggag tttctcgatc tggaaaccca gcgttatcaa ttgggcgtcg
    22081 atccatacgt cgacgtggtc acagcgcaaa ccacgctgct cggcgaccgg caaactttga
    22141 atgctctgca catctcgcaa atgatcgcga cggtgcagct ggtgcaggca ctgggcggcg
    22201 gatgggatcg ttcgcagctg ccatctccgg aacaagtcgg cgcaaaagcc ccgaacagcg
    22261 cttacaccat cgagaagtaa gcttcttcct gatcgcctgc agtgccactg acctgcaaac
    22321 ttcagttcgt ggtgcacctc gcccgtgcac ttcgcccgaa caccgccggc agagcggctt
    22381 tctgcggttt tcagcgtcca ggaccgttct tcttcagtac gaaattctct ctctaaccct
    22441 gaccacaggt cctcttttct gaaacgacag acaccttcgg aacaccagag ttctgttact
    22501 gcgaagcccc gaagttcgct caccaaagat tcgccggaaa caggcgttag atgttgattt
    22561 tcaatggtat aaacgagcat cgaacaagca gtaagacgta caaagaggac gtcgaatgct
    22621 gccttatctc tccgtgatta tttccgttcg caacgaacgg aaaatgctgc ctgggctca
//
LOCUS       DASZYN010000083         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_839487, whole genome shotgun sequence.
ACCESSION   DASZYN010000083 DASZYN010000000
VERSION     DASZYN010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839487"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VHE33_02185"
     CDS             <1..318
                     /locus_tag="VHE33_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT96286.1"
                     /translation="GDLVWLLVGFIFFFSISQGAVIWVYISEVFPTRVRSKGQSLGSS
                     SHWIMNAFISGLFPWVAAHSKAAPFGFFAAMMVLQFFVVLFMYPETKGVSLEQLQHKL
                     GID"
     gene            complement(400..1287)
                     /locus_tag="VHE33_02190"
     CDS             complement(400..1287)
                     /locus_tag="VHE33_02190"
                     /inference="COORDINATES: protein motif:HMM:NF014049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HVT96287.1"
                     /translation="MSDAKGPDSRPHQAPLRWQSQAVLLALIPFTAVWLTLEAGQLWY
                     AHDRSLWIGAGLCLLLAGVVFAARAATSGAALTGALIAGALWLQTPGWHTALWPLLAL
                     LTLTFSATRFGRRRKESLGIAEGRRGRTAAQVAANLGVAALAGIPLSVAHVFTPAAFG
                     GRAAMAARLTVMTAAMAEACADTLSSELGQVLGGTPRMITSLRRVPAGTDGAMSLAGT
                     LAGAIGAAVVVGVALLALPQLSRVDAAVAWAAGIAGTVVDSLLGAGPERQGWLNNDAV
                     NALSTLTAAVLAAWIVGRV"
     gene            1403..>2321
                     /locus_tag="VHE33_02195"
     CDS             1403..>2321
                     /locus_tag="VHE33_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96288.1"
                     /translation="MRLRCKASLLSRPVLNSQVRGTGILSHRIFAPVLAVAAAAGLAV
                     IVALPVGCGNQYRPVITPINPTGPAASPTAYITVVSQPGFSPLPATSASPCTGTQYAT
                     PAIYTLLDFAGDSVMAQANGGLGPLTFSLDSSGSTVYTPNCDGSLTTTTSTTTSLQTK
                     NVLTSTLLPGSEPTNTLLANGQLYVTEVGLHGPTCLTTNCVAQMTGLPSALKQEIPVA
                     PSLINLTGYGTAQRMYAISQGNSGGGAQPAWGDCATPSAVTATGEADAIETVSNTISA
                     RLPLGICPVYGFMTFDGQRTFIMNRGSGTV"
BASE COUNT          426 a          801 c          678 g          416 t
ORIGIN      
        1 ggcgacctcg tctggctgct ggtcggcttc atcttcttct tctcgatctc acagggtgcg
       61 gtcatctggg tttacatctc ggaagtcttc ccgacgcgcg tccgttccaa agggcagagc
      121 cttggttcca gctcgcactg gatcatgaac gcgttcatct cgggactttt cccctgggtt
      181 gcagcccact ccaaagccgc gccgtttgga ttctttgccg cgatgatggt tcttcagttc
      241 tttgtggtgc tgtttatgta tccggagacg aaaggcgtca gcctcgaaca gctccagcac
      301 aaactgggga tcgactagct tcgaagcata accctgatgg tgccccgtat ccctggtggg
      361 tgagttgtca gacagatcgc gctttctgtt gggagggagt caaactctcc caacgatcca
      421 ggcggcaagc acggcagccg tcagcgtcga cagagcattc acagcatcgt tattcagcca
      481 cccctggcgc tccggcccgg cgcccaggag ggagtcgacc accgtcccgg cgatccccgc
      541 cgcccacgca accgctgcat cgacccgcga caactgcggc agagccagca acgcgacccc
      601 caccacgact gcagccccga tcgcaccggc cagcgtgccc gccagggaca tcgcgccatc
      661 ggtacccgct ggaacacgcc gcagacttgt gatcatccgc ggcgttccac ccagcacctg
      721 gcccagctcc gacgaaagcg tatctgcaca ggcttcagcc atcgctgccg tcatgaccgt
      781 caggcgggcc gccatggccg cccgtccgcc gaacgccgcc ggcgtgaaca catgtgcgac
      841 cgagagcgga atcccagcca gcgccgcaac gcccaaattc gccgctacct gcgctgcggt
      901 ccgcccgcgc cgcccttcgg cgatacccag cgactccttg cgccggcggc caaaccgggt
      961 cgccgaaaac gtgagggtca gcagggcgag cagcggccaa agcgccgtgt gccatcccgg
     1021 agtctgcagc cacagagcgc cggcgatcag cgctccggtg agcgccgcgc ccgaagtggc
     1081 tgccctcgca gcgaagacca ctccggccag cagcagacac agccctgcgc cgatccacaa
     1141 cgaccgatcg tgtgcgtacc aaagctgacc cgcttcgagc gtcagccaga ccgcggtgaa
     1201 cgggatgagc gccagcagca ctgcctgcga ctgccatcgc agcggtgcct ggtgcggccg
     1261 agaatcggga ccctttgcgt cgctcatcgt ctataggtca tgcgcggcga tgtcgtgccg
     1321 cgtacagtac aatcatcttt tctaccagca acgagtgaaa agccgtgtcc ggaaatgccg
     1381 ggccggcctg aggagttggg tattgcgatt gcgttgtaag gcatctctgc tgagccgccc
     1441 cgtcctgaac agccaggtgc gcggaaccgg aatcctttct catcgaatct ttgcccccgt
     1501 ccttgccgtc gcagcagcgg cgggtctggc ggttatcgtt gccctgccgg tgggctgcgg
     1561 caaccagtat cggccggtca tcacccctat taatcccacg ggtccggccg catcgcctac
     1621 ggcatatatc acggtcgtgt cacagccggg cttctcaccc ctgccggcga cctccgcatc
     1681 gccctgcaca gggacgcaat acgcgacgcc agccatctac accctgctcg acttcgcggg
     1741 cgattcggtg atggcgcagg ccaatggcgg gctgggcccc ctgaccttct ctctggactc
     1801 ctccggttcc accgtctaca cgccgaattg cgacggctcc ctgacgacaa ccacctcgac
     1861 gaccacctct ctgcagacca aaaatgtgtt gacgagtaca ctgctgccgg gatcggagcc
     1921 gacgaacacg ctccttgcga atgggcagct gtatgtgacg gaagtcggac tgcacgggcc
     1981 tacttgcctg accaccaact gcgtggcaca gatgaccggc cttccctctg cgttgaagca
     2041 ggaaataccg gtggcgccgt cgctgatcaa tctcaccgga tacggtaccg cgcaacgcat
     2101 gtacgccatc agccaaggca attctggcgg cggagcgcag ccggcttggg gtgactgcgc
     2161 cactccttcg gcggtcacag caacgggcga agccgatgcc atcgagacgg tttcgaacac
     2221 gatttcagcg cggctgcccc tgggcatctg ccctgtgtac ggattcatga cgttcgatgg
     2281 gcagcgaacc ttcatcatga accgtggcag cggtacggtg a
//
LOCUS       DASZYN010000084         3822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_711066, whole genome shotgun sequence.
ACCESSION   DASZYN010000084 DASZYN010000000
VERSION     DASZYN010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711066"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VHE33_02200"
     CDS             complement(<1..573)
                     /locus_tag="VHE33_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96289.1"
                     /translation="MVAAISAELGWALGPIRTLFAGFAERRTACRAIARRATGLAEGG
                     TPFGPITRWLAVFAERWTNFGLIAALFAGFAERRTACRAIARRPAGLAERRRPCGALA
                     PGTISAGLSFLAEWCGAFGAAAPGRLACGAISLRRAVRRCCGAFRRTLPIAERPGREC
                     IFSALPGFVAAFLLTGTAGRTGTGRRTWAAA"
     gene            840..1544
                     /gene="cmk"
                     /locus_tag="VHE33_02205"
     CDS             840..1544
                     /gene="cmk"
                     /locus_tag="VHE33_02205"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HVT96290.1"
                     /translation="MNRGPIIAIDGPAGAGKSTVARHLAHHFGLLNLETGAMYRAFAL
                     KAIQCDQDFDDASALERLARDTEIRLEPDRSANRIYLDGQDVTERIRDADVTQGASRI
                     SVHPAIRAWLVDLQRELGRHGGIVMEGRDIGTVVFPDADVKVFLDASPEARTQRRYEQ
                     NSGNGTVAADSSDVFRELRERDERDRGRANSPLHPAPDAIVIDSTALSLAEVLARIED
                     LVRPLLARRGPMEKVS"
     gene            1594..1872
                     /locus_tag="VHE33_02210"
     CDS             1594..1872
                     /locus_tag="VHE33_02210"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HVT96291.1"
                     /translation="MIRTFEHKGLEKFFRTGSLAGIQAKHARRLRVQLAMLDQARQPE
                     DMNAPGWRLHPLQARLRDHWAISVSGNWRLTFRFEGEDAILVDYQDYH"
     gene            1891..2172
                     /locus_tag="VHE33_02215"
     CDS             1891..2172
                     /locus_tag="VHE33_02215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HVT96292.1"
                     /translation="MHNPPHPGEVIREYLGDISVSAAAEHLGVGRVTLSRVLNGNAAV
                     SPEMAIRLAAAFGTSTPEVWLGLQAQYDLWQVQKHNPIKVRPLPRLQQS"
     gene            complement(2249..>3822)
                     /locus_tag="VHE33_02220"
     CDS             complement(2249..>3822)
                     /locus_tag="VHE33_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HVT96293.1"
                     /translation="MASTPPSWTSREARWQAKQAARAQRAQWKAQMRAQKDYYRSYWR
                     GWHRPTFVGPLILLTIGVIALLMSTGKMDPGEFWSWYARWWPLLLIVMGGLLLGEHFL
                     DWNRPWAGRRSMGGIVWLVILMICLGWVSRNGHLMGPFSWEMGDDNDNFWNWMGQEHD
                     NDVQIDQVLTAAKPLITIDDPQGDVTITPSGDNQMHLRAHQMVHRNSDSEAQKLFEEL
                     KPKVEATGGGAVITVPEKQGARVDLTVELPAASYATVKTGRGDVTADGLTGGIQVSAN
                     HGEVKLEDIGGDAEGHISHGDFSAHNVQGRVLVDGLGDDVTLSQIQGPVTINGEFFGD
                     IHLEQIAAGVHFHSNQTTLEIPHLMGSMTLDKSDLSVSQAGGPVRVLAKSKDIDLTQI
                     AGDIHIEDTNGDVNIVAANPLGNLQITDHTGNVIVTMPENGNFTVTGSTSADEAIRTD
                     FPLKTSTDGGRQTLAGSVGHGGVQLQLETEHGNLELRKGDATTVSLTPPKPPTPPEAP
                     GKHFKAAPGTKSEEE"
BASE COUNT          704 a         1419 c         1020 g          679 t
ORIGIN      
        1 agcagcggcc cacgtccgtc ggccggtccc cgtccgccca gcggtccccg tcagcaaaaa
       61 ggcggcgaca aaccctggca gcgccgaaaa gatgcattcc cgcccaggcc gttccgcgat
      121 cggcagggtc cgccggaaag ctccgcagca gcgtcggacc gccctgcgca gggagatcgc
      181 cccgcaggca aggcgccctg gcgcggccgc cccgaacgcc ccgcaccatt cggcaagaaa
      241 cgacagcccg gcggagatcg tccctggcgc gagcgccccg cagggcctcc gccgttccgc
      301 aagaccggcg ggccgtcgag caatcgcgcg gcaggccgtc cgccgttccg caaagccggc
      361 gaacagcgcc gcgatcagac cgaagttcgt ccaccgttcc gcaaagaccg cgagccatcg
      421 cgtgatcggt ccgaaaggcg tccccccttc cgcaagaccg gtggcccgtc gagcaatcgc
      481 acggcaggcc gtccgccgtt ccgcaaagcc ggcgaacagc gtccggatcg gccccagcgc
      541 ccaccccaat tccgccgaga tcgcagcaac caaagccaga gcaggggccc gagtcagggc
      601 aagcgtccgt ggagcggccg tcccgcgttc ggtgcaaagc gcccaacttc tgccgaaggc
      661 gccggcaaac cctccgccgc gcccggacgc ccgtttcgtc ccggcaaacc cttcgccggc
      721 aaatcttttt ccggcaagcc cggccgttcc ggtccaccca aaaacaaatt tcgcaaatcg
      781 ttccccggaa aacaaaaacc gcgcaagaat cgtagtcaga accaggaaga gactcctgaa
      841 tgaatcgcgg cccgattatc gccatcgacg gcccggccgg cgcgggcaaa agcactgtgg
      901 cgcgccacct cgcccatcac ttcggtcttc tcaatctcga aaccggcgcc atgtaccgcg
      961 ccttcgccct caaggccatc cagtgcgatc aggacttcga cgacgcctcc gctctggaac
     1021 gcctggcgcg cgacaccgag atccgtctcg aacccgaccg ctccgccaat cgcatttatc
     1081 tcgatggcca ggacgtcacc gaacgcattc gcgatgccga tgtcacccag ggtgcttcac
     1141 gcatcagcgt ccatcctgcc attcgcgcat ggctcgtcga tctgcagcgc gagctcggcc
     1201 gccacggcgg cattgtgatg gaaggccgcg acatcggcac cgtcgtcttt cccgacgctg
     1261 atgtcaaagt cttcctcgat gcctcacccg aagcacgaac ccagcgccgc tacgagcaaa
     1321 actccggcaa cggtaccgtg gccgccgatt cttccgacgt ctttcgcgag ctgcgcgaac
     1381 gcgacgaacg cgaccgcggc cgcgcgaact ccccgctcca ccccgcgccg gacgccatcg
     1441 tcatcgactc caccgcgctc tctctcgccg aagtcctcgc ccgcatcgaa gacctcgtcc
     1501 gccccctcct cgcccgtcgc ggccccatgg aaaaagtcag ctgacggttt aattatcttg
     1561 ccttccgtat cgctacgcga tacactcctt ggtgtgatcc gcaccttcga gcacaaaggt
     1621 ctcgaaaagt tctttcgtac tggatcactg gcaggcattc aggcgaagca cgcccggcgc
     1681 cttcgtgtcc agctggcgat gctcgatcag gcccgccaac ctgaggatat gaacgcaccc
     1741 ggctggcggc tgcatccact gcaagcccgg ttgcgagatc attgggccat ctcagtcagc
     1801 ggaaactggc ggctcacatt caggtttgaa ggcgaagacg ctatcctggt cgattatcag
     1861 gactatcact aagggagtta agttatgcgc atgcacaatc cgccgcaccc cggcgaagtg
     1921 atccgcgagt atctcggcga cataagtgtc tccgccgccg ccgagcatct cggcgtcggt
     1981 cgcgtgaccc tgtcgcgcgt cctgaatggt aacgctgcag tgtctcccga gatggcaatc
     2041 cgcctggcgg ccgcattcgg cacttcaaca ccggaagttt ggctcggcct gcaggcgcag
     2101 tacgatctat ggcaagtgca gaagcataac cccatcaaag tccgtcccct gccgcgcctt
     2161 caacagtcct gaaccagagc gaacgcctgt ttcaaaatca aaacgccctc ccaaaaggag
     2221 ggcgttttag ttccacacca caccgcggct attcttcctc cgacttcgtg cccggcgcag
     2281 ccttaaaatg cttccccgga gcctccggcg gtgtcggcgg tttcggtggc gtcaacgaca
     2341 ccgtcgtcgc gtcacccttc cgcagttcca ggtttccatg ctcggtctcc agctgcaact
     2401 gcacgccgcc atgccccacc gagcccgcca gcgtctgccg tccgccatcg gttgaagtct
     2461 tcagaggaaa atccgttcgg atcgcctcat ccgcactcgt gctgcccgtg accgtaaagt
     2521 tcccattctc cggcatggtc acgatcacgt tccccgtgtg atcggtgatc tgcagattgc
     2581 ctaatggatt cgctgccacg atgttcacat cgccgttcgt atcctcgatg tgaatatccc
     2641 ccgcaatctg cgtcagatcg atgtccttcg atttagccag cacacgcacc ggaccccccg
     2701 cctggcttac gctcagatcg ctcttgtcca gcgtcatcga gcccatcaga tgcggaatct
     2761 ccagcgtcgt ctggttggag tggaagtgca cgcccgctgc gatctgctcc aggtgaatgt
     2821 cgccgaaaaa ctcgccattg atcgtcaccg gcccctggat ctgcgacagc gttacatcgt
     2881 cccccaaacc atccaccagc acccggccct gcacgttatg cgcggagaag tccccgtgac
     2941 tgatgtgccc ctccgcatcg cccccgatgt cctccagctt cacctcgccg tgattcgcgc
     3001 tcacctgaat gccgccggtg aggccatccg ccgtcacatc gccgcgcccc gtcttcaccg
     3061 tcgcgtagct tgccgcgggc agctccaccg tcagatcgac ccgcgcgccc tgtttctccg
     3121 gcaccgtaat gaccgcgccg ccgccggtcg cctccacctt cggcttcagc tcctcaaaca
     3181 gcttctgcgc ctcggagtcg gagttgcggt gcaccatctg gtgcgcccgc aggtgcatct
     3241 ggttatcccc ggaaggcgta atcgtgacat cgccctgcgg gtcgtcgatc gtgatcagcg
     3301 gctttgccgc cgtcagcacc tggtcgatct gcacatcgtt gtcgtgctcc tggcccatcc
     3361 agttccagaa gttatcgttg tcgtccccca tctcccacga gaacggtccc atcaggtgcc
     3421 cattccgcga cacccatccg agacagatca tcaggatcac cagccacacg atcccgccca
     3481 tcgagcgcct tccggcccat ggccggttcc agtccagaaa atgctcaccc agcagcaggc
     3541 cgcccatcac aatcagcagc agcggccacc accgcgcgta ccagctccag aactcgcccg
     3601 gatccatttt ccccgtcgac atcagcagtg cgatcacgcc aatcgtcagc aggatcagcg
     3661 gccccacaaa cgtcggccga tgccatccgc gccagtagct ccggtagtag tccttctgcg
     3721 cgcgcatctg cgccttccac tgtgcccgct gcgctcgcgc tgcctgtttg gcctgccacc
     3781 gcgcttcccg cgatgtccag gatggaggtg tggatgccat ct
//
LOCUS       DASZYN010000085         9157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_132560, whole genome shotgun sequence.
ACCESSION   DASZYN010000085 DASZYN010000000
VERSION     DASZYN010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 9157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9157
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132560"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..890
                     /locus_tag="VHE33_02225"
     CDS             282..890
                     /locus_tag="VHE33_02225"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HVT96294.1"
                     /translation="MRRFPILTAALLALTLSAHAAKVGDPAPDFTATDSHGQTHKLSE
                     YRGRFVVLEWTNSNCPFTKKHYTSGNMQALQKEWTAKGVVWLTVLSSAPGNDGYKTAA
                     EENAWVEQIHAAPTAVLLDPSGALGHQYEAKTTPDMFVIDPNGKLIYSGAIDDRRTVD
                     PSDVKGAKNYVADALTEAMAGQPIQIAYTRSYGCSVKYTGTD"
     gene            complement(947..1477)
                     /locus_tag="VHE33_02230"
     CDS             complement(947..1477)
                     /locus_tag="VHE33_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HVT96295.1"
                     /translation="MDDSDARTQQLVAGLGAEQLNWQPQPGAWSVGQCLEHLCMTHEA
                     YLPAIAAALPGKPVSPVQEITPGWFGRWFMRSFIEPSPATSRTRAPRKIVPGTGPVDP
                     SILDRFLKSTQAIRETLVCAQDLNVNRIRFKNPLIGVIHFTVGTGFQILSGHLRRHLL
                     QAERVKDAPGFPGRVS"
     gene            complement(1619..2314)
                     /locus_tag="VHE33_02235"
     CDS             complement(1619..2314)
                     /locus_tag="VHE33_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HVT96296.1"
                     /translation="MVTNIAAVERSGFKSAARIHRTLTAGAEKRLLLWMAERAPRWVT
                     SDGLTALGLSAQVAAGACYALARWDRRALIPGIGFIVLNWLGDSLDGTLARVRQQQRP
                     RYGFYVDHVVDIFGSAALLGGLAVSGLVHGWVAAAMLVTFLLLAGESYLATYTLGRFE
                     MSQGWFGPTELRILLIAANLRLLFSPWITVLGHRWMLFDVGGAIGAAGMLGMAVMVAA
                     RHTAQLYREEPVP"
     gene            complement(2320..3159)
                     /locus_tag="VHE33_02240"
     CDS             complement(2320..3159)
                     /locus_tag="VHE33_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96297.1"
                     /translation="MKRTIAGLGMGLGILMALTPAGAWAEAPAPAVAAFDGYVGALEA
                     RLAHAHRSREGFLTGDASMAEGRAQLRHGEVVIEQVKTPELKGAMLHDWRGSAFVPGA
                     TAAEFEELLRDFDADARVFAPEVMSGRTLLADGDHVQGMMRVRQKHVITVVLDTTYDV
                     RFGRLDPEDGWNASRSMRVDEIADVGTSHERALRPEEEHGFLWRINTYWTWQERDGGL
                     YVQVESVSLTRGIPMGLGWIVGPFVESVPRESLAFTLAAASRAMAGTSRKPEPGVVAM
                     GNR"
     gene            3329..4198
                     /locus_tag="VHE33_02245"
     CDS             3329..4198
                     /locus_tag="VHE33_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HVT96298.1"
                     /translation="MSENPVSEHPRRYRFGAYEADAATGELRRQGIRLRLNAQPFQVL
                     CLLLDRPGELLTRDEIAHALWPDGVNVDADHGVNSAVNRIREALGDSASSPRFIETLA
                     RRGYRFVAPVERIGDGSIPSTALSADAPEAAPSPDQPRHILTTPEELPTVPHPVPRNL
                     FLGFQLMYLGFYIGALANLAEIQDLLAPLTHPVTVFMTLIVTAAILIPVRAFLFCAVL
                     FRAPRFRDKYLRVWPFLLPFDVLWSLSPFLLLHHINFGLALAATALLAWSPFVQRSLI
                     LMGAGDPQVSTDH"
     gene            4262..5692
                     /locus_tag="VHE33_02250"
     CDS             4262..5692
                     /locus_tag="VHE33_02250"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HVT96299.1"
                     /translation="MPQHASEGSESGHKLHRALGLGDLVLAQILCVVGTSWVGVAGGL
                     GPAQSLTWVAAMLVFYVPMAASVICLNRAMPLEGGLYVWARHAYGDLGGFLTAWNLWF
                     YGIAVVASILYAMPTEISYLIGPSAAWLPENRPVSLVIVVVMLAGIMFAALRGLELGK
                     WIHNIGGIAILIVFAALILLPLVAYARHQPVRYAPFTIAAPPHDLRMLALFGQMLFGA
                     LCGLEYIAILAGESRDPARSIGRSVVISSPVICAMFILGTASIIALVPRARIDFIAPI
                     PQAMRLTLGNSGVANLAAMLAILLLQFRLLGATSYLFTGVTRLPMAAGWNHLVPAWFT
                     RLHPRRATPTNSILCTTAIALALVLLASTGVHAQEAYQLLTNASLTHYEVAYLAMFAI
                     PLVGAAALRRSLPRWLKWTSVAGFLATLFSLLISAYPFVNVVNAHIYAIKILSTILVS
                     NLIAFCFYRLRTRTAARDKAALSRAA"
     gene            5745..5842
                     /gene="ffs"
                     /locus_tag="VHE33_02255"
     ncRNA           5745..5842
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VHE33_02255"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5991..6983
                     /locus_tag="VHE33_02260"
     CDS             5991..6983
                     /locus_tag="VHE33_02260"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HVT96300.1"
                     /translation="MPVRAKISALGTWTPPRLLTNADLERMVETSDDWITSRVGIKER
                     HIVEPGMATSHMAVEAAKLCLAERGIDPTDVEVIIVGTVTPDMLFPATACLVQDKLGA
                     KGAWGFDLSAACSGFVYSLQMGCKLVESGAHKKVLVIGADTMSSIIDYTDRATCVLFG
                     DGAGAVLIEPAAEGEYGMIDFHHEIDGSGACSLYMPGGGSLHPSTHETVDKKMHFVHQ
                     DGQAVYKFAVRKMVEATETVLKRNGVTGADLGCFVAHQANKRIILGTSERLGLPEEKV
                     IINIDRFGNTTCGTIPLAMETARQEGKLNKGDLVLIASVGAGFTVGAALLRWEC"
     gene            complement(7047..7271)
                     /locus_tag="VHE33_02265"
     CDS             complement(7047..7271)
                     /locus_tag="VHE33_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96301.1"
                     /translation="MKAATIVGVLLIVLGIIGFVAGGFSFTHEKKDIDMGPVQVEHKS
                     TKTVPISPILSTIALVGGIGLVVVGVRSKA"
     gene            7440..8153
                     /locus_tag="VHE33_02270"
     CDS             7440..8153
                     /locus_tag="VHE33_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HVT96302.1"
                     /translation="MPPEIHWPAIALRLVLTVLAGAALGTDRSRNGHPAGLRTTLLVT
                     LAASVAMIQMNLLISTNGKPPNSYAVMDLMRLPLGILTGVGFIGAGAIVRKGDMVLGV
                     TTAATLWFSTVVGLCLGGGQLVLGCVATVIGYLTLTSLRWFERRLEKFQPATLVLVTS
                     GSSLTAEDLRAQLQAARFRVRSISIDHSPEDRRLRLRCEVRWPADSSSAALPNILRNL
                     ERTPGLVRLSWHTVSTSSH"
     gene            8228..>9157
                     /locus_tag="VHE33_02275"
     CDS             8228..>9157
                     /locus_tag="VHE33_02275"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HVT96303.1"
                     /translation="MASRQFRLRHGCVCACVSTLFALTGCGQFFPPLTSGGGGGGGTS
                     TGDYLYVGNLGTNPLSIAGFSIGTTSLSGISGSNWSVELEPTALAVTPTDTYLYLGSA
                     AGGIYVYTIGSNGALTIANNGSPVATGVSPSVLRVDPTGKWLLGASAFAGQAYVYQIG
                     SGGSLTAISSSVVTLNASTPATDLEITPNGNYVFVSCGTAGIYEMSFSTGSGALAQIN
                     GVLNPKTNGAADMGLAVSPSSNYLFAAETVTGGVRVFSIGTNGVLTETSGSPYTTNTG
                     AWSVLVDSTGSYVYVANRTAGTISAFLLTNTGAL"
BASE COUNT         1616 a         3518 c         2314 g         1709 t
ORIGIN      
        1 cgcgaatgca tcactcgcgc gtgcgacgga agtgacctgg ctccagtcag atggctcgtt
       61 gtcaaacttg tcaactccgc cgaacttgcc cggaatcaat caactccacg ctcgcccccg
      121 aaaaaaagcg agcctcaaac cctggcccaa ccgtcactcc cttgtagacc atcccggatc
      181 cctgcgcgtc caatctccca acaagcggcc aacgctcccc ggattccgtc gccgcccggc
      241 ccgaagccaa cctccctgac ctcgacccca ggagcttctc catgcgccgc ttccccatcc
      301 tgaccgccgc tctcctcgcc ctcaccctct ccgcccatgc cgcgaaggtt ggcgaccccg
      361 ctcccgactt caccgccacc gattcccacg gccaaaccca caagctctcc gagtaccgcg
      421 gcaggttcgt cgtcctcgag tggaccaaca gcaactgccc ctttacgaaa aagcactaca
      481 ccagcggcaa catgcaggcc ctgcagaagg agtggaccgc gaagggcgtc gtctggctca
      541 ccgtcctctc ctccgccccc ggcaacgacg gctacaagac ggctgccgag gagaatgcct
      601 gggtcgaaca gattcacgcc gctcccaccg ccgtcctgct cgaccccagc ggtgccctcg
      661 gccaccagta cgaagcgaaa accacgcccg acatgtttgt catcgatccc aacggcaagc
      721 tgatctactc cggcgccatc gacgaccgtc gtaccgtcga cccctccgac gtaaagggag
      781 caaagaacta cgtcgcagac gccctgacag aagccatggc cggtcaaccc atccaaatcg
      841 cctacacccg ctcctacggc tgcagcgtca agtacaccgg gaccgattag acgcaacaga
      901 acaatgcggt ggcagccctc gccccacagc ttgatggccg cgcgatctag gagacccggc
      961 caggaaaacc cggggcatcc ttcacccttt ccgcctgcag cagatgccgc cgcaggtggc
     1021 cggagaggat ttggaagccg gttccgacgg tgaagtgaat cactccgatg agcgggtttt
     1081 tgaagcggat gcgattcaca ttgaggtcct gagcacacac aagggtttct cggatggcct
     1141 gggtgctctt caggaaacga tcgaggatgg acggatcgac aggtccggtt ccgggaacga
     1201 tcttcctggg cgcgcgcgtg cgcgaggtcg caggagacgg ttcgatgaag ctgcgcatga
     1261 accacctgcc aaaccatccc ggagtgatct cctggactgg agaaaccggc ttgcctggga
     1321 gcgcagcggc gatcgctggc agataagcct catgggtcat gcacaggtgc tcgaggcatt
     1381 ggcccacact ccaggcccct ggctgcggct gccagttgag ctgttcggca ccgagtcccg
     1441 ccaccagttg ttgggttcga gcgtcgctgt catccaactc ggcgttcagc cgaactcgcc
     1501 acgcgggagc gggactctga cctgggtgcg tcacaaccct aaggatactg gagacacgaa
     1561 ccgctatctg cccccgctct gccgagccac ctcgcgatct cccacgcacc aggggaactc
     1621 acggcaccgg ctcctccctg tacagctgcg ccgtgtgccg agccgccacc atcaccgcca
     1681 tccccagcat tcccgcggct ccgatcgctc cacccacatc gaacagcatc catcgatgcc
     1741 cgagtaccgt gatccacgga ctgaacagca gtcgcagatt ggccgcgatc agcagaatcc
     1801 gcagctccgt cggcccaaac catccctgcg acatctcgaa ccgccccagc gtgtacgtcg
     1861 ccagatagct ctcgccagca aggagcaaaa acgtcaccag catcgccgcc gctacccacc
     1921 cgtgcaccag acccgagacc gccagtccac ccagcagcgc tgccgacccg aagatgtcca
     1981 cgacgtgatc cacgtagaag ccataccgcg gccgctgctg ttgacgtacc cgcgccagcg
     2041 ttccgtccag gctgtcgccc agccagttca gaacgatgaa cccaatgccg ggaatcaatg
     2101 cgcgccgatc ccatcgcgcc agggcatagc aggcgcccgc cgcaacctgc gcgctcagcc
     2161 ccagcgccgt caatccatcc gacgtcaccc accgcggagc ccgctccgcc atccacagca
     2221 gcaagcgctt ctccgcaccg gctgtcagcg ttcgatggat tcgagccgcg cttttgaacc
     2281 ccgatctctc caccgctgca atatttgtaa ccatcaccgt cacctattcc ccatcgccac
     2341 gactcccggt tccggctttc tgctggtccc tgccatggcc ctcgacgcag cggccagcgt
     2401 gaacgccagc gactcccgag gcacgctctc cacaaagggc ccgacgatcc accccagccc
     2461 catcggaatt ccccgcgtca gcgataccga ctccacctgc acatacagcc cgccatcgcg
     2521 ctcctgccaa gtccagtagg tgttgatccg ccacagaaac ccgtgctcct cctccggcct
     2581 cagcgctcgc tcgtggcttg tgcccacatc tgcgatctcg tccacccgca tactccgcga
     2641 tgcgttccac ccgtcctcgg gatccagccg cccgaaccgc acgtcatagg ttgtgtccag
     2701 caccaccgtg atcacgtgct tctgccgcac ccgcatcatg ccctgcacat gatcgccgtc
     2761 cgccagcagc gtccgccccg acatcacctc cggcgcaaac acccgcgcat cagcgtcgaa
     2821 gtcccgcagc aactcttcaa actccgccgc cgtcgctccc ggcacaaacg ccgacccgcg
     2881 ccagtcgtgc aacatagccc ctttcagctc cggcgtcttc acctgctcga tcacgacctc
     2941 accgtgccgc aactgtgccc gcccctccgc catcgatgca tcaccggtca gaaacccttc
     3001 cctcgaccga tgcgcatgcg ccagccgtgc ctccagcgcc ccaacatacc catcgaacgc
     3061 cgccaccgcc ggcgcgggag cctcagccca cgcgcccgcc ggcgtcagcg ccatcaatat
     3121 gccgagaccc atacccaacc ctgcaatcgt ccgcttcatg tccccaacat ccccccacca
     3181 gcccttcgag gcaatgcaaa gcggctctct tgttacagga atgtttctca cggcgcaaac
     3241 cgcacgaatc gcgcgacttg ttccgagttc tccatcggcc cggcccgcgg caaacgctcc
     3301 accatcgact acacttgtcc ccgagccgat gtccgaaaac cccgtatccg agcacccccg
     3361 ccgctaccgc ttcggagcct acgaagccga cgccgccacg ggcgagctgc gccgtcaggg
     3421 cattcgtctc cgcctcaacg cccagccctt ccaggttctt tgcctcctgc tcgatcgccc
     3481 cggcgagctt ctcacccgcg acgaaatcgc ccacgccctc tggcccgatg gcgtgaacgt
     3541 ggacgccgac catggcgtca actccgccgt caaccgcatc cgcgaagccc tcggcgactc
     3601 cgcctccagt ccgcgcttca tcgagaccct ggcccgccgc ggctaccgct tcgtcgcgcc
     3661 cgtggagcgc atcggcgatg gatccatccc ttcaaccgcc ctctctgccg atgcgccgga
     3721 agccgcgccc tcccccgatc agccccgcca catcctcacc accccggaag aactccccac
     3781 cgtcccgcat ccggtcccgc gcaacctctt cctcggcttc cagctcatgt acctcggctt
     3841 ctacattgga gccctcgcca accttgccga aattcaggac ctgctcgcgc ccctgaccca
     3901 ccccgtcacc gtcttcatga ccctcatcgt cacggccgcc atcctcattc ccgtccgcgc
     3961 cttcctcttc tgcgccgtcc tctttcgcgc tcctcgcttc cgcgacaaat acctccgcgt
     4021 ctggcccttc ctcctcccct tcgacgtcct ctggtcgctc tctcccttcc tgctcctcca
     4081 ccacatcaat ttcggactcg cgctcgccgc caccgccctc ctggcctggt cacccttcgt
     4141 gcagcgctcg ctcatcctca tgggcgccgg agatccgcag gtctccaccg atcactgatt
     4201 tctgagccct gtgcccagtg ccctgcagtc tttgccctga tcgtgcgacc atgttcgaac
     4261 catgccccag catgcctccg agggctctga atcaggccac aaactccacc gcgctctcgg
     4321 tcttggtgac ctcgttctcg cccagattct ctgcgtcgtc ggcacctcct gggtcggcgt
     4381 cgctggcggg ctcggcccag cccaatccct cacctgggtc gccgccatgc tcgtcttcta
     4441 cgtgcccatg gcggcctccg tcatctgcct caaccgcgcc atgcccctcg aaggtggcct
     4501 ctacgtctgg gcccgccacg cttacggcga tctcggtggc ttcctcaccg catggaacct
     4561 ctggttctac ggaatcgctg tcgtcgcttc gatcctctat gccatgccga cagagatctc
     4621 gtatctcatc ggtccctccg ccgcgtggct cccggaaaac cgcccggtct ctctggtcat
     4681 cgttgtcgtg atgctcgccg gcatcatgtt cgccgcgctc cgtggcctcg agctcggcaa
     4741 gtggattcac aacatcggcg gcatcgccat cctcatcgtc ttcgccgcgc tgattctgct
     4801 gccgctcgtc gcctatgcgc gccatcagcc cgttcgctat gcgcccttca ccatcgccgc
     4861 tccaccgcac gacctgcgca tgctcgccct cttcgggcaa atgctcttcg gcgctctgtg
     4921 cggccttgaa tacatcgcca tcctcgccgg cgaaagccgc gaccccgcgc gatccatcgg
     4981 acgctctgtc gtgatttcct cgcccgtcat ctgcgcgatg ttcatcctcg gcacggcgtc
     5041 catcatcgct ctcgttcccc gcgctcgcat tgacttcatc gcgcccattc cccaggccat
     5101 gcgcctgacc ctcggcaact ccggcgtggc gaacctcgcc gccatgctcg ccatcctgct
     5161 tctccagttt cgcctcctcg gagccaccag ctatctcttc accggcgtca cccgcctccc
     5221 catggccgcg ggctggaatc acctggtccc ggcatggttc acgcggctac atccccgccg
     5281 cgccacaccc acgaactcca tcctctgcac aaccgccatc gctttggccc tcgtcctcct
     5341 cgccagcacc ggcgtgcatg cgcaggaggc ctaccaactc ctcaccaatg ccagcctgac
     5401 ccattacgag gtcgcttacc tcgccatgtt cgccattccg ctggttggcg ccgccgctct
     5461 gcgccgctcc cttccgcgct ggctcaagtg gacctccgta gccggattcc tcgcgacgct
     5521 cttcagcctg ctgatctcgg cctatccctt tgtgaacgtc gtcaacgccc acatctacgc
     5581 catcaagatc ctctcgacga tcctcgtctc gaatctcatc gcgttctgct tctatcgcct
     5641 tcgcacccgg accgcagcaa gagacaaggc cgcgctttcg cgcgccgcgt gagacgctgc
     5701 ccctccactg tctcatccgt atcgcaatgc taaaattgcc tgtgtgccgg gtcctgcgcg
     5761 acgcaatccc gccaaccccg ccaggtccgg aaggaagcaa cggtaacggg ccagtgcgtg
     5821 cgcagcaggt cacccggtgc actaagatcc agtcccaacc ccaggttagt cctctccact
     5881 ccgcttcctc tgtcaccgtc cgcacgatga accgccccgg ccacggcact ccgctaaaat
     5941 agtcctttgc tttcaggagc cttcatttga atctttctct tcgtccgcgt gtgcccgttc
     6001 gtgccaaaat cagcgccctc ggaacctgga cgcctccccg cctcctcacc aacgccgatc
     6061 tggaaagaat ggtcgagacc tccgacgact ggatcaccag ccgtgtcggc atcaaggagc
     6121 gccacatcgt cgagcccggc atggccacca gccacatggc tgtcgaagcc gcaaaactct
     6181 gcctcgccga gcgcggcatc gatcccaccg acgtcgaagt catcatcgtc ggcaccgtca
     6241 cccccgacat gcttttccct gccaccgcct gcctcgtgca ggacaaactc ggcgcaaagg
     6301 gcgcctgggg attcgacctg tccgcagcgt gctccggctt cgtctactcg ctccagatgg
     6361 gttgcaagct cgtcgagtca ggcgcacaca aaaaagttct cgttatcggc gccgacacca
     6421 tgtcgtcgat cattgactac accgatcgcg ccacctgcgt cctgttcggc gacggcgccg
     6481 gcgcagtcct catcgaaccc gccgcggaag gcgaatacgg catgatcgac ttccaccacg
     6541 aaatcgacgg ctcgggcgcg tgttcgctct acatgcccgg cggcggcagt ttgcatccct
     6601 cgacccatga aaccgtcgac aagaagatgc acttcgtcca tcaggacggc caggccgtct
     6661 acaagttcgc cgtgcgcaag atggtcgaag ctacggagac agtcctcaag cgtaacggcg
     6721 tcaccggcgc cgatctcggc tgcttcgtcg cccaccaggc gaacaaacgg atcatccttg
     6781 gaacttcgga gcgcctcgga ctgcccgaag aaaaggtcat tatcaacatc gaccgcttcg
     6841 gcaacaccac ctgtggcacc atcccgctcg cgatggagac agcccgccag gaaggcaagc
     6901 tcaacaaggg tgacctcgtc ctgatcgcca gcgttggtgc tggctttacc gtcggcgcgg
     6961 ctttgttgcg ctgggaatgc tgaatcggcg ttcggttgtg aggcatcagc gttcaggccg
     7021 gttggttctg aacgctgaac ctttcgctaa gccttgcttc tgactcccac caccaccagc
     7081 ccaataccac ccaccagcgc gatggtgctc aggatcggtg agatcggcac agtcttcgtc
     7141 gacttgtgct cgacctggac cggccccata tcgatgtctt tcttttcgtg ggtgaagctg
     7201 aaccctcccg ccacaaatcc gatgatcccc aggacaatca ggagcacacc aacaatcgtt
     7261 gctgctttca aatcgttcct cccttgatgg ggccgtgact gtggaaactc gcccggccgc
     7321 ctcagggtga gatgcctgcc ggacagaacg cgcaccccaa atttcgccga gcgagttgcc
     7381 tgatcgctaa gggatttagc caaccactgc ttcaccgcac gcatcgcacc gctgtcatca
     7441 tgccgccgga aattcactgg ccggccatcg ctctgcgcct cgtcctcacc gtgcttgcag
     7501 gcgccgcgct tggcacagac cgcagccgta acggtcatcc cgctggacta cgcaccactc
     7561 tcctcgtcac gctcgcggca tctgtggcca tgatccagat gaacctgctt atttccacaa
     7621 acgggaagcc gcccaactcc tacgccgtga tggatctcat gcgcctgccc cttggcatcc
     7681 tcaccggtgt tgggttcatc ggcgccggag cgattgttcg caagggcgac atggttcttg
     7741 gcgtcacgac cgcggcaacc ctctggttct ccaccgtcgt cggcctttgc ctcggcggcg
     7801 gccagcttgt cctgggctgc gttgcaacgg tcatcggcta tctcaccctg accagcctgc
     7861 gctggttcga gcgtcgcctc gagaagttcc agccggcgac gctcgtcctg gtcaccagcg
     7921 ggtcgtcgct cacggctgag gatctgcgcg cacaactcca ggccgcccgc tttcgcgtcc
     7981 gctccatctc gatcgaccac tcaccggaag atcggcgcct ccgcctccgc tgcgaggttc
     8041 ggtggcccgc cgactcaagc tccgccgccc tgcccaacat cctgcggaat ctggagcgga
     8101 cacctggact cgtgcgtctc tcctggcaca cagtttccac ctcgtcacat taaggagcag
     8161 aacccccggg ggctgcgtct ctatggatgc cgcgcaccag cggcgatacg atagtcctga
     8221 ggtaagaatg gcttcccgtc agttccggct ccgtcacggc tgtgtgtgcg cgtgtgtatc
     8281 gactctgttt gccctcaccg gctgcggcca gttctttccc cctctcacca gtggtggagg
     8341 cggtggcggc ggcacatcga ccggggacta cctttacgtc ggaaacctcg gcaccaatcc
     8401 gctgagcatc gctggcttct cgatcggaac cacctccctg agcggtatat ccggctcgaa
     8461 ttggtctgtt gagctggagc ccacagctct ggcagtcacc ccgaccgaca cctacctcta
     8521 tctgggcagc gctgcgggtg gcatctacgt ctacaccatc ggctccaatg gggctcttac
     8581 catcgccaac aacggatcgc ctgttgccac cggagtctct ccctccgtcc tccgggtgga
     8641 ccccacaggt aaatggctcc tcggggcatc cgccttcgcg ggccaggcct acgtctacca
     8701 aatcggcagt ggcggctctc tgaccgccat cagctcctcg gtggtcaccc tgaacgccag
     8761 tactcccgcc acagacctcg agatcacgcc taacggcaat tacgtcttcg tctcctgcgg
     8821 tacggcaggc atctacgaga tgagcttcag caccggtagt ggcgcgctcg cccagatcaa
     8881 cggggtcctc aatccgaaga ccaacggggc tgcagacatg ggcctggcag tttcgccatc
     8941 cagcaactac ctctttgctg ccgaaaccgt caccggaggc gtgcgtgtct tctccatcgg
     9001 aacgaacgga gtccttaccg aaaccagcgg ctcaccctac acaacgaaca ccggcgcctg
     9061 gtcggtgctg gtcgattcca cgggaagcta cgtctatgtc gccaaccgta ccgcgggaac
     9121 gatcagtgcc ttcctcctca cgaacacagg tgcgctc
//
LOCUS       DASZYN010000086        10246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_580066, whole genome shotgun sequence.
ACCESSION   DASZYN010000086 DASZYN010000000
VERSION     DASZYN010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 10246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10246
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580066"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VHE33_02280"
     CDS             <1..454
                     /locus_tag="VHE33_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96304.1"
                     /translation="YGPGFMGWAKLSGVTLTEDMTDEFVGTFLIPSIDHQDPHYHRMP
                     NASMPRRILHAAVQPFWTQSDTGKGMVNYSTVVGSFIEEAVNVSYVPYQQMGWGPAAA
                     RISIGWASAPIGNYVTEFVPDLASHVNLRVVFLQRIVDRVARESVGAP"
     gene            complement(464..1726)
                     /locus_tag="VHE33_02285"
     CDS             complement(464..1726)
                     /locus_tag="VHE33_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HVT96305.1"
                     /translation="MNSAAVLFAAARSHLLCPLPPEKLAQAIALSRWHAVFYFGGTLW
                     ILLALWTLVRLRAGAAIAGFAERRTSRPWLQGFVVIPAWVVLYCAIAAPIAILSHRVS
                     VGYGLSIERWDAWWVDWLKSSLITIGVGVVVIGILYAILRRTPRRWWIWFWAITIPLE
                     VVATWAAPLVLDPMFNHFTPLANTNPALVDQLERVAARGGLDIPRDRIFLMDASKRVT
                     GANAYVTGLGGSKRIVVWDTTLKIETPNEILFTYGHEQGHYVLHHVAQGIAWSALVIF
                     VFYWFAYRAMRWLVLRYGERLRIAAVESWSSLGLFLVVAYGLNFLAQPVANLISRHIE
                     HQADVYGQEVIHGLVPNVQATVVASFCSDALVWLDDPEPNRFVEFWTYNHPSTEERAE
                     FASHYDPWQAGQRPKYVRSEDGTPNPAR"
     gene            complement(1731..2534)
                     /locus_tag="VHE33_02290"
     CDS             complement(1731..2534)
                     /locus_tag="VHE33_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT96306.1"
                     /translation="MTALIVDDEELAREELKYLLDAAGSVDVVGEGKNGIEAVELIRT
                     HHPDVVFLDVQMPGLDGFAVLKKLLEFGDREQLPQIIFATAYDQYAVRAFDVNAIDYL
                     LKPFDRDRVLQALERARLRLQEAGSSGEHRPAQSDVRVDTLLKLIEQHQASKPHSGKI
                     VLQAGTRLLLIDQKDICYASIDEGVISVVTPTIEGQSKCRTLEELFDLLDPATFWRAH
                     RSFLVNINHIREVVPWFKSSYQLRMDDKKQTEVPVSRAQTRRLRELFNL"
     gene            complement(3424..3819)
                     /locus_tag="VHE33_02295"
     CDS             complement(3424..3819)
                     /locus_tag="VHE33_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVT96307.1"
                     /translation="MSDLVKKVQEMYAAFGRGDVETILNNTTEDVSWEFEAPAALTWS
                     GIRKGRQEAMGFFTGIAAEHANPVLTMTDYFSTNDAVAAFGRYEATVKATGKRVNTPV
                     GHYFKFRDGKVSRYINLINSGAFLEAAKK"
     gene            complement(3859..4347)
                     /locus_tag="VHE33_02300"
     CDS             complement(3859..4347)
                     /locus_tag="VHE33_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HVT96308.1"
                     /translation="MKRWITVAWMTMAIAMAIGCNRVSDTAADVQAIQETEKQWNQDY
                     ASKDADKLAAYYSDDAVLMAPGMAPSVGRESIRATLKAMVADPALSLKFTAAKIDVAG
                     SGDLGYTRGAYVMTMTDPQTKKVVQDHGSYVTTYRKDTEGKWKAAADIATSEAAPGQS
                     SM"
     gene            complement(4430..5002)
                     /locus_tag="VHE33_02305"
     CDS             complement(4430..5002)
                     /locus_tag="VHE33_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96309.1"
                     /translation="MTQLITRWKPATTATLLSLGLAIPSASAKCFKDFSQVLKTPVVS
                     AAVAAPSGQLATNNNNFNHDSFGANGIVGLWTATFTSGGQTVDVAFDAWHSDGIEVLN
                     DYTNPIEGNVCLGVWTQTGPRTFKLKHPSWAFDTTGTLVGTVLIRETVTLGLDGNSYK
                     GNYSYDIYDTTGAFVTELTGTVAATRIRPE"
     gene            5209..7113
                     /locus_tag="VHE33_02310"
     CDS             5209..7113
                     /locus_tag="VHE33_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HVT96310.1"
                     /translation="MPIETRELYEFGDFRLDAARRLVTRLEQPLRLNSKAFETLLVLV
                     RNRRRVVSKDELMQTLWPDTFVEEVNLAQNISAIRKALGETPGENRYIATIPGKGYQF
                     VCQVDEIGAEREGATAEPSPPMAPTSELAAIRPSPTVLARPGARWITWATIGPVLVLG
                     AAGIWWFRHGAATDAAMATPHSVAVLPFQPLAGGSDEEHLGLGMADAVITRLSNIRQL
                     PVRPTDVVIRYGDPAVDPLKAAREMGVDTVLTGRLQKSGDRVRVTVQLVRVADGRPLW
                     AQTFDDQYTSIFQVEDSISEKVAQALALNLGASEKLQLQRHYTDNIDAYRNYLMGRYQ
                     EFTFTRDGMNKAIDYFNRAISEDPGYALAYAGLADAWTTESDWLISPREAMPKADAAA
                     RKALAFDDQLAEAHGALAHALLHEWKLADSNREFQTALALNPGNVSTYFAYGEYLASV
                     GKADEAIAQGKKALAIDPLSPEINSFLPWDYYLKRDYGNCLASAQRAIQMFPDFWLPH
                     MTAGICYSFSGEYDSGIREFSKALAMNPDSTFAQAGIAMCLAKAGRRAEALRALDRLQ
                     AMKDHTYVSPSYVSLVYLTLGDRDAEYAWLEKAYDDRAEFLLWLTVDPIYDGEQKDPR
                     FQAMVRKVGL"
     gene            7175..7249
                     /locus_tag="VHE33_02315"
     tRNA            7175..7249
                     /locus_tag="VHE33_02315"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7207..7209,aa:Val,seq:cac)
     gene            complement(7385..9946)
                     /locus_tag="VHE33_02320"
     CDS             complement(7385..9946)
                     /locus_tag="VHE33_02320"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HVT96311.1"
                     /translation="MLLQRSPAQQADLENLVARQQQPGTPEYHKWLTPAEFGARFGAS
                     PQDVATLSSWLESQGFRVRGVLKNSSMIDFEATAAQVREAFHSQLHYVNMRGGKYSAA
                     IQDPQIPAALAPVVVGIKGLNKIPPLTNHTEPHPSSRDSATHSWQMIVPQTDDTVTPA
                     FNAGGGYYVVSPQDLYTIYKVNPVFTSGNLGANATVAVIEETDIEYGTVNSGTGSATG
                     GDIATFRSLFGVPGTLNMHVYHGFGSVTCSDPGIVANEDVEATLDAEWMNATAPSANL
                     IFMSCDNTTNDGIFSSMAALIDNNLSDVMSLSYGESEQYYTASDYAFQDNLFAQAAAQ
                     GQSIVISSGDSGSDVRDQNTSTTATHGINVSAFAAPLVTVTGGTDFQDLYDTLEGGSP
                     QSAYWSSTNSAAFGDALGYVPEMPWNDSCASSILAVYEGYTGAGLCATNSYTNGDVVG
                     GSGGISTHYAVPSWQSGISGYSNSMHSQPDISGIAADGFWGHYLVFCDSNPSYSGSYD
                     CSSSSNFGGAGGTSFVAPYMAGIFGLLRTATGSRQGLLNPALYALAKAQYTSPSTQTA
                     CYANGQTSNTGVTTGLPASTCIFNDVTTGNNDVPCASGRPNCYVNSGKTYGMLSLTGS
                     SSLSVAYPSGVGYDQVTGIGTVNVANLIAGWNTAFASSTTLQANPTTISATQSTQLTA
                     TVSGGTPTGYVDAPPSVTGTITFKAGSTVVGTCTLSAGTCSTGVPGSSLQSGANSLTA
                     IFGGSGTYPSSTSSVVTVTVQNSSPTPLTPWIQVNNGGWQQTNIVTVSPGSMVNLGEQ
                     VLTGGSYSWTGPGGYSSSSRIASNVPLTSTSNVFTLTYTNTVGATSTQSFTITVN"
     gene            9881..10069
                     /locus_tag="VHE33_02325"
     CDS             9881..10069
                     /locus_tag="VHE33_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96312.1"
                     /translation="MLLASNQILKVGLLRRAALQEQKEVKGLFIFHQTKIWCAKNVLA
                     NIPTEKRGDFVVDRLHQT"
BASE COUNT         2005 a         3073 c         3059 g         2109 t
ORIGIN      
        1 ctacggcccc ggcttcatgg gatgggcgaa gctcagcggc gtcaccctca ccgaagacat
       61 gaccgacgag ttcgtcggca ccttcctcat cccctccatc gatcatcagg acccgcacta
      121 tcaccgcatg ccgaacgcct ccatgccgcg gcgcatcctg catgccgccg tgcagccctt
      181 ctggacccaa agcgacaccg gcaagggcat ggtcaactac tccaccgtgg tcggctcgtt
      241 catcgaggaa gccgtcaacg tcagctacgt tccctaccag cagatgggat ggggaccggc
      301 agcggcccgc atcagcatcg gctgggccag cgctcctatc ggcaactacg tcaccgagtt
      361 cgtgcccgac ctcgccagcc acgtcaacct gcgcgtcgtc ttcctgcagc gaatcgtcga
      421 ccgcgtggcg cgcgaaagcg tcggcgcgcc ctaaacaaag cgttcagcgc gcggggttcg
      481 gcgttccgtc ttcacttcgc acatactttg gcctctgccc agcctgccac ggatcgtagt
      541 gcgacgcgaa ctccgcccgc tcttccgttg acggatggtt atacgtccag aactccacaa
      601 accggttcgg ctccggatca tccagccata ccaatgcatc gctgcaaaat gatgccacca
      661 ccgtcgcctg cacattcggc actagcccgt ggatcacttc ctggccgtaa acatccgcct
      721 gatgctcaat gtgccggctg atcaggttcg ccaccggctg cgccagaaag ttcagcccat
      781 acgccaccac cagaaacagc cccagcgacg accagctttc aaccgcggcg atccgcagcc
      841 tttctccata gcgaagcacc agccaccgca tcgcccgata cgcaaaccag tagaagacga
      901 agatcaccag cgccgaccac gcgattccct gcgccacatg gtgcagcacg tagtgcccct
      961 gctcgtgacc gtaggtgaac agaatttcgt tcggcgtctc gatcttcagc gtcgtgtccc
     1021 acaccacgat gcgcttgctc ccgcccagtc ccgtcacata agcgttcgct ccggtcacgc
     1081 gcttgctcgc gtccatcagg aagatccggt cgcgaggaat gtccagcccg cctcgcgccg
     1141 ccacccgctc caactgatcc accagcgccg gattcgtgtt cgccagcggc gtgaagtggt
     1201 tgaacatcgg atcgagcacc agcggcgccg cccacgtcgc caccacctcc agcggaatcg
     1261 tgatcgccca gaaccagatc caccaccgcc gcggcgtgcg ccgcaggatc gcatacagaa
     1321 tgccgatgac caccacaccc acgccgatcg tgatcaggct ggatttgagc cagtccaccc
     1381 accacgcgtc ccagcgctca atcgacagcc cgtaccccac gctcacgcga tggctcagga
     1441 tcgcaatcgg cgcggcgatc gcgcagtaga gcacgaccca cgcggggatg acgacgaagc
     1501 cctgcagcca cggccgcgac gttctgcgtt cggcaaatcc cgcaatggcc gcgcccgcgc
     1561 gcagacgcac cagcgtccac agcgccagca ggatccacag cgttccgccg aagtagaaca
     1621 ccgcatgcca gcggctcagc gcaatcgcct gcgccagctt ttcgggaggc aatgggcaga
     1681 gcaggtgcga acgcgcggcg gcgaacagaa cggctgccga gttcacgccg ctaaagattg
     1741 aacagctccc gcagccggcg cgtctgcgcc cggctcaccg ggacctcggt ttgcttctta
     1801 tcgtccatgc gcagctgata gctggacttg aaccacggca ccacttcccg gatgtggttg
     1861 atgttgacca ggaacgaccg gtgcgcgcgc cagaacgtcg ccgggtcgag caggtcgaac
     1921 agctcttcca gcgtccggca cttcgactgg ccttcaatcg tcggcgtcac caccgagatc
     1981 acgccctcgt cgatcgaggc gtagcagatg tccttctggt cgatgagcag cagccgtgtg
     2041 ccggcctgca gcacgatctt gccgctgtgc ggcttcgatg cctggtgctg ctcgatcagc
     2101 ttcagcagcg tgtccacgcg aacgtcgctc tgcgccggcc ggtgttcgcc cgaggagccc
     2161 gcctcctgta gacgaagccg cgcgcgttcg agagcctgga gaacccgatc gcggtcaaac
     2221 ggcttcagca ggtagtcgat cgcgttcacg tcgaaagccc gcaccgcgta ctgatcgtaa
     2281 gcggtggcga agatgatctg cggaagctgt tcgcgatccc caaactcaag cagctttttc
     2341 agaaccgcga atccgtccag ccccggcatc tgcacgtcga gaaagacaac atcggggtga
     2401 tgcgtgcgga tcagctcaac cgcctcgatc ccgttcttgc cctcgccgac cacatcgaca
     2461 ctgccggcgg catcgagcag gtacttcagc tcctcgcgag ccagctcctc atcatcgaca
     2521 atgagcgcgg tcatagacat gcactctgag tatagaaggc gcggcagtcg gtaaaaagcg
     2581 ctgctcagtt gtgatgctcc tttggcgaga aaaatctatt tttgggggtt gacccctcgg
     2641 aatgttgttg attccagaga agcgcgtctt tgttggaccc cctcgttgtt agcagatgcg
     2701 ttaactgcgt cagaatgtgc agtcggcggc cgcgaatgac tccaggatga gggtgttggc
     2761 gcgggcgacg attcgtgtgc agggttcgtg cgtggtattg agtctccttt ctgacggtca
     2821 agaattcgat tcgtcgattc gcgttttgtg cgccgacttg tcgcgatgtt gtgatttgtt
     2881 cttaaggaca cgttgtaagt tccctgtttc gaattacaga tcgcagttta caaacttgac
     2941 aactttagcc gaggctcgcc ccgcgcggta cctatgaccc gtggaccggg tggggctggg
     3001 cagcattcgt gtctcctctc gagcaacaga agaggctcgc ctcggcgagc ctttcctgtt
     3061 acgaaaccta agtgggagag cagagttttg tatctatctc cctattatca gaatagcaga
     3121 ttgagggaat aatcgtgcca acgtttttgg gtggttttgg cttctaagtg gttgttcctc
     3181 agcggtcgtt actttggatt ttctccacag ctcttgacag cgtttcagga aagcgcaagc
     3241 agtcactata gcgacccgac gatgtcacct tcgcaacgga aacgtgcttt cagcagggct
     3301 gaagccatgc cccttcacgt ttccgttacc aaggtcagag gagcaaggca gactacggcc
     3361 gtgccctgac acttttcgat gccgagggcc aggccgaagc ttggtagtcg ataaattctg
     3421 acgctacttc tttgccgcct ctaaaaatgc gcccgaattg atgagattga tgtaccggct
     3481 taccttcccg tcgcggaatt tgaagtagtg tccaaccggg gtgttcacgc gcttgcccgt
     3541 ggctttcact gtcgcctcgt atcggccaaa cgccgccacc gcgtcgttgg tcgagaagta
     3601 atccgtcatg gtcagcacag gattcgcatg ctctgcggca atcccggtaa agaagcccat
     3661 cgcctcctgc cgccccttgc ggatgccgct ccacgtcagc gctgcaggcg cctcgaactc
     3721 ccagctaacg tcctcggtgg tgttgttcag aatggtctcc acatctccgc gcccaaatgc
     3781 cgcgtacatt tcctgcacct tcttcaccag gtccgacatg ctcgttctcc cttgcgtgat
     3841 ttgcactgca gccattccct acatcgaact ctgtcccggc gctgcttccg acgtcgcgat
     3901 atcggccgcc gccttccact tcccttccgt atccttgcga tacgtcgtga catagctgcc
     3961 gtggtcctgg actaccttct tcgtctgcgg atcggtcatc gtcatgacgt aggcgccgcg
     4021 cgtgtagccc agatcgcccg agccggctac gtcgatcttc gccgccgtga acttgagcga
     4081 caacgccgga tcggccacca tcgccttcag cgtcgctcga atgctctccc gacccaccga
     4141 gggggccatg cccggagcca tcagcaccgc gtcatcgcta tagtaggctg ccagtttgtc
     4201 cgcatccttc gacgcgtagt cctggttcca ttgtttttca gtctcctgaa tcgcctgcac
     4261 atccgccgcc gtatcggata cgcgattgca accgatggcc atcgcgatcg ccatcgtcat
     4321 ccacgccact gttatccacc gcttcatttg tttctccttg atgtgcgaga tggactgctt
     4381 ctcgggagga ggctggggga tacctctccc gagtcctcag gaaggcccgt tactccggcc
     4441 ggatgcgcgt ggccgcgacc gttccggtca gctcagtcac aaacgcgccg gttgtgtcgt
     4501 aaatgtcgta gctgtagttg cctttgtagc tgttgccatc gaggccgagg gtcacggttt
     4561 cccggatgag gaccgtgccc accagagtgc ccgtagtatc gaacgcccat gacgggtgct
     4621 tcaatttgaa ggtgcgcggg ccggtctggg tccacacgcc caggcagaca ttgccctcga
     4681 tggggttggt gtagtcgttg agcacctcga tcccgtcact gtgccaggcg tcgaaggcca
     4741 cgtcgacagt ctggccgccc gaggtgaagg tcgcggtcca caagccgacg atgccgtttg
     4801 cgccgaacga gtcgtggttg aagttgttgt tattggtcgc cagctggccg gacggcgcag
     4861 cgaccgccgc cgagacgacc ggcgtcttga gcacctggct gaaatcttta aagcatttgg
     4921 cggaagccga gggaatggcc agtcccaggc tgaggagggt ggctgtggtg gctggcttcc
     4981 atcgggttat gagttgagtc atcaagaatc ggggcatccc ggcgatcgtc atcatggttt
     5041 tccttccctc tttcggttcg tgaatcggat tggccgggca gcggccgcgg ttcctctgcc
     5101 caacgccgca catcgttatc gaagagggtc ggatttgtct tgtgatgggc acttaggatt
     5161 tgttaggatt tcctgcattc gcgcggatgc aggtccaccc acgcctgtat gcctatagaa
     5221 acaagggagt tatacgagtt cggagacttc cggctggacg ccgcccgccg cctggtcacc
     5281 cggctggagc agcccctccg tctgaactcg aaagccttcg agacgctcct tgttctggtg
     5341 cggaaccgtc gccgcgtggt ctcgaaagac gagctgatgc agaccctgtg gccggacacc
     5401 tttgtggagg aggtcaatct cgcccagaac atctccgcga tccgcaaagc gctgggcgag
     5461 acgccgggcg agaaccgcta catcgcgaca atcccaggca aggggtatca gttcgtttgc
     5521 caggtggacg agatcggagc ggaacgagaa ggagcgaccg cagaaccatc ccctcccatg
     5581 gctccgacat ccgagttggc ggcgatccgt ccttcgccca ctgttttggc gcgtcccggc
     5641 gctcggtgga tcacgtgggc gacgattggg ccggtgctgg tgctgggggc tgcgggtata
     5701 tggtggtttc ggcatggcgc ggctacggat gcggcgatgg cgacgcccca ctcggttgcg
     5761 gtgctgccct tccagccgct ggctgggggt tcggatgagg agcatctcgg cctgggcatg
     5821 gccgatgcgg tcattacgcg gctttcgaac atccggcagc tgccggttcg gcccaccgat
     5881 gtagtgattc gctatggcga tccggccgtc gatcctctaa aggcggctcg ggagatgggc
     5941 gttgacacgg tgctgacggg ccggttgcag aagtcggggg accgggtgcg cgtcacggtg
     6001 cagctggtcc gcgtggccga tgggcggcca ttgtgggcgc agacttttga tgatcagtac
     6061 acgagcatct tccaggtcga ggactcgatc tcggaaaagg tcgcgcaggc gctggcgctg
     6121 aatctggggg ccagcgagaa gctgcagttg cagcgccact acaccgacaa tatcgatgcc
     6181 tatcgcaact atctgatggg ccgctatcag gagttcacgt ttacgcgcga tgggatgaac
     6241 aaagcgatcg actacttcaa tcgcgcgatc tcggaggatc cgggttacgc gcttgcgtac
     6301 gcggggctcg ccgatgcctg gacgacagag tcggactggc tgatctcacc gcgggaagcg
     6361 atgccaaagg ccgatgcggc tgcgcgcaag gcgctggcgt tcgacgatca gctcgcggaa
     6421 gcacacggcg cgctggcgca cgcactgctg catgagtgga agctcgccga ttcgaatcgc
     6481 gagttccaga cagcgctggc tttgaatccc ggcaacgtat cgacgtactt cgcctatggc
     6541 gagtatttgg cgtcggtggg taaggcggac gaggccatcg cgcaggggaa gaaggcactg
     6601 gcgatcgacc ccctgtcgcc ggagatcaac tccttcctgc catgggacta ttacctgaag
     6661 cgcgactacg gcaactgtct ggcatcggcg caacgagcca ttcagatgtt tccagacttc
     6721 tggttgccgc acatgacggc gggaatctgc tattcgtttt caggggagta cgactccggg
     6781 attcgcgagt tcagcaaagc gctggcgatg aacccggata gcactttcgc gcaggctggt
     6841 attgccatgt gcctcgcgaa ggccggccga cgcgcagaag cgctgcgggc gttggatcgg
     6901 ctgcaggcga tgaaggatca tacctacgtt tcgcccagct atgtgtcgct cgtgtacctg
     6961 acgctgggcg atcgcgacgc cgagtacgca tggctggaga aggcctacga tgatcgcgcc
     7021 gagttcctgc tttggctgac cgtcgatccg atctacgatg gggaacaaaa ggatccgcgc
     7081 tttcaggcga tggtgcgcaa ggtagggctc tgatgcggcg ctgctgccgt gttaatcttg
     7141 aatctgtccg agccctatcc cggggtggaa ttccgggcgc ataactcagc ggtagagtgc
     7201 caccttcaca cggtggaagt cgtaggttcg aatcctgctg cgcccaccaa atcattcttg
     7261 agaaaactgg aatcgagatt ttcacctgga ggtgccggca tccagactcc cgggcgcgcg
     7321 gccaagaggc atttcggatt atattattta taatcaatca cttacatgaa cacgacgatg
     7381 atcgtcagtt gaccgtgatc gtgaatgatt gtgtgctggt cgctccaacc gtgttggtgt
     7441 aggtgagcgt gaagacgttg ctggttgatg tcagcggcac attcgaggcg atccgcgagc
     7501 tggagctata gccccctggt cctgtccagc tgtagctgcc accggtcagc acctgctcgc
     7561 caagattcac catgctgccc ggactcacgg ttacgatgtt ggtctgctgc caacctccgt
     7621 tgttcacctg gatccagggg gtcagcggcg ttggtgagct attctggacc gtcacggtga
     7681 caacgctgga cgtggacgac gggtaggttc cgcttcctcc gaagattgca gtcagggaat
     7741 tagcgcccga ctggagcgat gaaccgggta ctccagtaga acaggttccg gcgctgaggg
     7801 tgcaggtgcc cacgacagtg cttcctgctt tgaaggtgat tgtgccagtc accgaagggg
     7861 gagcgtcgac ataacctgta ggagttccac ctgaaacggt cgccgtcaat tgcgtggact
     7921 gagtcgcact gatggtggtg ggattggcct gcagtgttgt gctgctggcg aatgcggtat
     7981 tccaaccagc gatcaaattc gcgacgttaa ccgtgccgat tccggtaacc tggtcatacc
     8041 ccacgcccga gggataggcc accgacaacg aactggagcc ggtcaacgat aacatgccat
     8101 aagttttgcc ggagtttacg tagcagtttg gccgccccga cgcgcagggg acatcattat
     8161 tgccggtggt gacgtcgttg aagatgcacg tggacgcggg caagccggtc gtgacgcccg
     8221 tgttcgacgt ctggccattg gcgtaacagg cggtttgcgt tgacggcgaa gtatattggg
     8281 ccttagccag cgcatagaga gcgggattga gcaggccctg acgagaaccg gtcgccgttc
     8341 taagcagacc aaagatgccc gccatataag gcgcaacaaa agacgtgccg ccagctccgc
     8401 cgaagtttga actggaagag cagtcataag atccgctgta actgggattc gaatcgcaga
     8461 agaccaggta gtgcccccag aatccatcgg cggcgattcc agaaatatct ggctgcgagt
     8521 gcatagaatt ggagtaaccc gagatgccgg actgccagga aggtactgca tagtgtgtgc
     8581 tgatgccgcc cgaaccgccc accacgtcgc cgttggtata tgagttggtg gcgcaaaggc
     8641 cagcacccgt ataaccctca tagaccgcga ggatgctgct ggcgcaactg tcattccatg
     8701 gcatttcagg aacgtaaccc aaagcatcgc caaaagcggc agagttcgtc gagctccagt
     8761 aagcgctctg cggcgaaccg ccttccaacg tatcgtaaag gtcctggaag tcagtgcctc
     8821 ctgtaaccgt gaccaatggg gcggcgaagg cgctcacgtt tatcccgtgc gtagctgtcg
     8881 tgctggtgtt ctggtccctg acgtcggaac ccgagtcgcc agaagagatg acgatggatt
     8941 ggccctgcgc tgcggcctgg gcgaacaaat tgtcctggaa agcgtaatcc gacgccgtgt
     9001 aatactgttc gctctctcca taactaaggc tcatgacatc ggagaggttg ttatcgatca
     9061 gcgcagccat cgagctgaaa ataccgtcgt tcgtggtgtt gtcgcaagac atgaagatga
     9121 ggttagcgga cggagcggtt gcgttcatcc actcagcgtc cagcgtcgcc tctacatctt
     9181 cgttggccac tatgccgggg tcgctgcagg tgaccgatcc gaagccgtga tagacgtgca
     9241 tgttcagcgt cccgggcaca ccaaacaggg accggaaggt agcaatatcg ccgccggtcg
     9301 ccgaccctgt gcctgagttc acggtgccgt attcgatgtc agtctcttcg atgacagcaa
     9361 cggtggcatt cgcgcccaga ttgccgctcg tgaaaaccgg attgacttta taaatggtat
     9421 agagatcctg agggctcacg acgtagtatc cgccgcccgc attgaacgca ggtgtgaccg
     9481 tgtcgtcggt ttgagggacg atcatttgcc aactatgggt agccgaatcc cgggacgacg
     9541 ggtgaggctc ggtgtggttg gtcagcggag gaatcttatt gagccccttg atccccacaa
     9601 ccaccggagc cagtgccgct gggatctgcg ggtcttgaat cgccgcgctg tacttgcctc
     9661 cccgcatgtt gacgtaatgc aactggctgt ggaaggcttc gcgaacctga gctgcggtcg
     9721 cctcaaagtc aatcatgctg ctgttcttga ggacgccgcg aactctgaat ccctgcgatt
     9781 caagccagct tgacagcgtt gcaacgtcct gcggtgaagc accgaaacgg gcgccgaatt
     9841 ccgccggggt gagccatttg tgatattcgg gagttcccgg ttgctgctgg cgagcaacca
     9901 gattctcaag gtcggcttgc tgcgcagggc tgcgctgcag gagcagaaag aggtgaaggg
     9961 gcttttcatc ttccaccaaa ccaagatctg gtgcgcgaag aacgtcctgg cgaacattcc
    10021 cactgagaag cgtggtgact ttgtcgtcga taggcttcac cagacgtgac ggaatcgggg
    10081 aaaaaccagc agaattctgg cgatccgact cccgctgagc agaggccgag tcgctaaacg
    10141 atagcaggat gatcgcaaaa agaatgaaag agcttgcaga gaagtggcgg aatggaaggc
    10201 gagagacgat catcaaagct cccctggtcc ggatgctgac tcggag
//
LOCUS       DASZYN010000087         2822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1096597, whole genome shotgun sequence.
ACCESSION   DASZYN010000087 DASZYN010000000
VERSION     DASZYN010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096597"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..>2822
                     /locus_tag="VHE33_02330"
     CDS             187..>2822
                     /locus_tag="VHE33_02330"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96313.1"
                     /translation="MFEFLRKCAPAVRCFASLTATLVLSAPVVLAQQTSAPLAKFDDS
                     GPAAGLEALTLRQIGPFRGGRVDAVSGVPGEPLVYYFGGTGGGVFKTTNGGHTWEPIT
                     DGKINFGSIGSIAVADSDHNVIYVGTGESAIRGNASHGDGVYKSMDAGKTWTHVGLED
                     TQQIGMIRIDPRDPNLVYVAALGHMAGPNADRGVFRSKDGGKTWQKVLFKSDKAGAID
                     LAMDPSNSRVLYASFWQVVRHPWTFESGGPDSGIWKSTDGGDTWTDITHNEGLPKGVL
                     GRIGVTVSPVNPERVWALIEAQEGGIYRSDDAGKTWHKENGDNSIKQRAWYYSQVFAD
                     PKDANTVYAVNTSLFRSTDGGHTFTTIRNQHGDNHDMWIAPEDPQRFIESSDGGAQIS
                     FDGGKSWSTEDNQPTGQFYRVSVDNDFPYHIYGAQQDNSTVATLSRGNSGSITTSDWH
                     DVGGGESGWVVADPTNSRYVYAGSYDGLLTRYDRVTDSERDINVWPDNPMGSGVEAMK
                     YRFQWTFPLVFSPHNPKRLYAGANVLLATTDEGQHWTVMSPDLTRNDKSKQGPSGGPI
                     TKDNTAVEYYDTIFTVDESPVKAGVIWVGSDDGLIHVTQDDGKSWKNVTPKDMPEWIR
                     INCIAASPFDAGTAYVAATMYLSDDFHPFLYRTTDYGRTWTKIVSGIADDDFTRAIRP
                     DPNQKGLVFAGTESHLYISYDDGDHWLPFQLNLPAVPVTDITFQKQWDDMVLATQGRG
                     FYVMDEMPLVRALNPAHFQKSAAAVLFPVKQAIRIDGGGGFGRPAPDEGANPPSGAVI
                     YYWLKDKPEGEVKIRILTADGKLVREVSSKPAKDTGVEDEDEGPRKPPPAPAKAGLNR
                     YVWNLRYEDATGFPGLLMWDGSL"
BASE COUNT          537 a          704 c         1054 g          527 t
ORIGIN      
        1 tgttcctttt gatcgctctt tgctgtgagc tgtgtcgctc gctaccttgc ccccgtttgg
       61 cctgctgata cttcctgctg ttcttctgag acttattggc gctcacatcc gtttttctga
      121 ataaggtccc cagccgtttc cagatcctgt acgtccgcat tccacttccg aagggagttc
      181 gatctcgtgt ttgaatttct gcgcaaatgt gccccggctg ttcgatgttt cgcatctctg
      241 acggcaacgc tggtgctcag cgcgccggtc gtgttggccc agcagacgtc tgcgccgctg
      301 gcgaagttcg atgacagcgg tccggcggcc ggacttgaag cgctgacact gcggcagatt
      361 gggccgtttc gcggagggcg cgtagatgcg gtaagcggcg tgccgggcga gccgctggtg
      421 tactactttg gcggaacggg cggcggcgtg ttcaagacga cgaacggcgg ccacacgtgg
      481 gagccgatca ctgatggcaa gatcaacttc ggatcgatcg ggtcgattgc tgtcgccgat
      541 tcggatcaca acgtgatcta tgtgggcacg ggcgagagcg cgatccgcgg caacgcgtcg
      601 catggcgatg gcgtgtacaa gtcgatggac gcgggcaaga cgtggacgca tgtggggctt
      661 gaggacacgc agcagattgg gatgatccgc atcgatccgc gcgatccgaa cctggtgtat
      721 gtggcggcgc tggggcacat ggcggggccg aatgcggatc ggggcgtgtt tcggtcgaag
      781 gacggcggga agacgtggca gaaggtgctg ttcaagagcg acaaggcggg cgcgattgat
      841 ctggccatgg atccgagcaa ttcgcgggtg ctgtatgcga gcttctggca ggtggtgcgg
      901 catccgtgga cgtttgagag cggcgggccg gacagcggca tctggaagtc gaccgatggg
      961 ggcgatacgt ggacggacat cacgcacaac gaggggttgc cgaagggggt gctggggcgc
     1021 attggggtga cggtgtcgcc ggtgaacccg gagcgggtgt gggcgctgat tgaggcgcag
     1081 gagggcggaa tttaccgctc ggacgatgcc gggaagacgt ggcacaagga aaacggcgac
     1141 aacagcatca agcaaagggc gtggtactac tcgcaggtgt tcgccgatcc gaaggacgcg
     1201 aacacggtgt atgcggtgaa cacgtcgctg ttccggtcga cggacggggg gcacacgttc
     1261 acgacgattc gtaaccagca cggtgacaat cacgacatgt ggattgcgcc ggaggatccg
     1321 cagcggttca tcgagtcgtc ggacggcggg gcgcagatca gcttcgatgg cggcaagagc
     1381 tggagcacgg aggacaacca gccgacgggg cagttctatc gggtatcggt ggacaacgac
     1441 tttccgtacc acatctacgg cgcgcagcag gacaacagca cggtggcgac gctgagccgg
     1501 gggaattcag gatcgattac gaccagcgac tggcacgatg tgggcggcgg cgagagcgga
     1561 tgggtggtgg cggatccgac gaactcgcgg tatgtgtacg cgggatcgta tgacggattg
     1621 ctgacgcgct atgaccgggt gacggattcg gagcgcgaca tcaatgtgtg gccggataat
     1681 ccgatggggt cgggcgtgga ggcgatgaag taccgcttcc agtggacctt cccgctggtg
     1741 ttttcgccgc acaatcccaa gcggctgtat gcgggagcga atgtgctgct ggccacgact
     1801 gacgaggggc agcactggac ggtgatgagc ccggacctga cgcggaatga caagtcgaag
     1861 caggggccgt cgggcgggcc gatcacgaaa gacaacacgg cggtggagta ctacgacacg
     1921 atcttcacgg tggatgagtc gccggtgaag gcgggcgtga tctgggtagg ttcggacgat
     1981 gggctgattc acgtgacgca ggacgatggg aagagctgga agaacgtcac gccgaaggac
     2041 atgccggagt ggatccggat taactgcatt gcggcgtcac cgttcgatgc ggggacggcg
     2101 tatgtggcgg cgacgatgta tttgtcggac gactttcatc cgttcctgta ccggacgacg
     2161 gactatggca ggacatggac gaagatcgtg agcgggattg cggatgacga tttcacgcgc
     2221 gcgatccggc cggatccgaa ccagaagggg ctggtgtttg cggggacgga gtcgcatctg
     2281 tacatctcgt acgatgacgg cgaccactgg ctgccgttcc agctcaacct gccggcggtg
     2341 ccggtgacgg acattacgtt ccagaagcaa tgggacgata tggtgctggc gacgcagggg
     2401 cgtggcttct atgtgatgga cgagatgccg ctggtgcggg cgttgaatcc ggcgcatttc
     2461 cagaagagcg cggcggcggt gctgttcccg gtgaagcagg cgattcgcat cgatggcggc
     2521 ggcggatttg gacggccggc accggatgag ggggcgaatc cgccgagcgg cgcggtcatt
     2581 tactactggc tgaaggacaa gccggagggc gaggtcaaga tccgcatcct cacagcggat
     2641 gggaagctgg tgcgggaggt gtcgagcaag ccggcgaagg acacgggtgt ggaggacgag
     2701 gatgaggggc cgaggaagcc gccgccggcg cctgcgaagg ctgggctgaa tcggtatgtg
     2761 tggaatttgc ggtatgagga tgcgacgggc tttcctggat tgctgatgtg ggatggttcg
     2821 ct
//
LOCUS       DASZYN010000088         2551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1158552, whole genome shotgun sequence.
ACCESSION   DASZYN010000088 DASZYN010000000
VERSION     DASZYN010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158552"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /locus_tag="VHE33_02335"
     CDS             complement(<1..1392)
                     /locus_tag="VHE33_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96314.1"
                     /translation="MATKVSNPAGSDFAQQLREWIEAFDDVVVAEGPEQGGELLTALK
                     QRAREAGISQASELTTPYANTIPKHEEVPYPGDRKLERRIEALIRWNAMAMVHGQNKK
                     DHGIGGHIATYSSLATLLEVGFNHFFHAKHGDQPGDFVYFQGHASPGIYARAFLLGRL
                     DEQRILNFRHELRETPGLSSYPHPWLMPDFWRFPTVSMGIGPLNAVYQARFMRYLENR
                     GLIEKTPRKIWAFVGDGETDEVDTLGALSLGRREKLDNLIFVVNCNLQRLDGPVRGNA
                     RIIDELEAVFLGAGWNVIKVIWGSDWDELFERDHTGLLLKRMEECVDGEYQAFKAKGG
                     AYIRKEFFGKYPELLKLVEHMTDDEIFHLHRGGHDAKKIYNAYKRAVEHKGGPTVILA
                     KTVKGYGLGSTQARNASHQEKKVADDALVDFIQRYDLPIPEADARAGKLYRPAADSPE
                     MAYMHARRQGLGGF"
     gene            complement(1416..2282)
                     /locus_tag="VHE33_02340"
     CDS             complement(1416..2282)
                     /locus_tag="VHE33_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVT96315.1"
                     /translation="MATEAGTRVTSATAHGKHGHSLISDAKFRQLYDLALRMQHAGER
                     EQGLRGREAALAGVTADLRDDDVVVAEYAGSVEDITQGHVAVSMERRTFEERVIEALS
                     DAVGDRMRKTGRITAIFFEGTASSRVMQEARAMAIAAKLPVLLVEHLSTKRQRDASTE
                     KSLRTVLEYPSIPVDAQDVIAMYRVAHESIERARDGGGPTHIVSVRWQVAGSAGKRAG
                     KAATQDAVQHLEEWLTARGLPAREWRRAMIAELEKGAREGAGRSQELAFDARNASDRE
                     AEDEGAEARAIA"
BASE COUNT          433 a          962 c          639 g          517 t
ORIGIN      
        1 aaagccaccc agcccctgcc gccgtgcatg catgtacgcc atctccggcg agtcggccgc
       61 cggacgatac agcttgcccg cccgcgcgtc cgcttccgga atcggcaagt cgtagcgctg
      121 gatgaagtcc accagcgcat cgtcggccac tttcttttcc tggtgagaag cgttgcgcgc
      181 ctgcgtgctg cccagaccgt atcccttcac tgtcttggcc aggatcaccg tcggcccgcc
      241 cttgtgctcc accgcccgct tgtaggcgtt gtagatcttc ttcgcatcat gaccaccgcg
      301 atgcagatgg aaaatctcat catcggtcat gtgttcgacc agcttcagca gttccggata
      361 cttgccgaag aattccttcc ggatgtacgc gccgcccttt gccttgaacg cctgatactc
      421 gccgtccaca cactcttcca tgcgcttcag cagcagcccg gtgtgatccc gctcgaacag
      481 ctcgtcccag tccgaacccc agatcacctt gatcacgttc cagcccgcgc cgaggaacac
      541 cgcttccagc tcgtcgatga tccgcgcgtt gccgcgcacc ggcccatcga gccgctgcag
      601 attgcagttc accacgaaga tcaggttgtc gagcttctct ctccggccca gcgacagcgc
      661 gcccagcgtg tcgacctcgt ccgtttcacc atcacccaca aacgcccaga tcttgcgcgg
      721 tgttttctcg atcagcccgc ggttctccag ataccgcata aaccgcgcct ggtacaccgc
      781 gttcagcggc ccaatcccca tcgacaccgt cgggaaccgc cagaaatccg gcatcagcca
      841 tgggtgcgga tacgacgaca gccccggcgt ctcgcgcaac tcgtgacgga aattcaggat
      901 ccgctgctca tcgagccgcc ccagcagaaa cgcccgcgca taaatccccg ggctggcgtg
      961 tccctggaag taaacgaagt cgcctggctg atcgccgtgc ttcgcgtgaa agaagtggtt
     1021 gaacccgact tccagcagcg ttgccagcga cgaatacgtt gcgatatgcc cgccgatgcc
     1081 gtggtccttc ttgttctgcc catgcaccat cgccatcgcg ttccagcgaa tcagcgcttc
     1141 gatgcgccgc tccagtttgc gatcgcccgg atacggaacc tcttcgtgct tcggaatggt
     1201 gttcgcatag ggcgtcgtca gttcgctcgc ctgcgaaatg cccgcctcgc gcgcacgctg
     1261 cttcaaggct gtcagcagct cgccgccctg ttccggcccc tccgccacaa ccacatcgtc
     1321 gaaggcctcg atccactccc gaagctgctg cgcaaaatcg ctccctgccg gattgctaac
     1381 cttagtcgcc ataaactcct gatgatttct ccgcgctacg cgattgcgcg cgcctctgcc
     1441 ccttcgtctt ccgcctctcg gtcggatgca ttccgggcgt caaaagccag ctcttggctc
     1501 cgccctgcgc cctctcgcgc gcccttctcc aactccgcga tcatcgcgcg ccgccattcc
     1561 cgcgccggca gaccccgcgc tgtcagccac tcttccagat gctgcaccgc atcctgcgtt
     1621 gcggctttcc cggctcgttt gcccgcgctc ccggccacct gccaccgcac gctcacgata
     1681 tgggtcgggc cgccgccatc gcgagcccgc tcaatcgact catgcgccac gcggtacatc
     1741 gcaatcacat cctgcgcatc caccggaatg gagggatact ccaacaccgt ccggaggctc
     1801 ttctccgttg acgcatcccg ctgccgcttc gtcgacagat gctccaccag cagcaccggc
     1861 aacttcgccg cgatcgccat cgcgcgtgcc tcctgcatga cccggctgct cgcggttccc
     1921 tcaaaaaaga tcgccgtgat ccggcccgtc tttcgcatgc gatcccctac ggcatcgctc
     1981 agcgcctcga tcacccgctc ctcaaaagtt cgtcgctcca tcgaaaccgc aacatgtccc
     2041 tgcgtgatgt cctcaaccga tccggcatat tcggccacca ccacatcgtc gtcccgcaga
     2101 tcggcggtta cgcccgccag cgccgcctca cgcccgcgca gcccttgctc acgctcaccg
     2161 gcgtgctgca ttctcagcgc cagatcgtag agctgccgga atttcgcatc gctgatcagc
     2221 gaatggccat gcttgccatg ggccgtcgca gacgtaaccc gcgttcccgc ctctgtcgcc
     2281 atctgttcta agtgatcctc ttcgaccgct gctcggggga tcgcggtcct ggctgctgca
     2341 agcgtttcca tgcgccgctc gcgctataga aaaattgtag cgcacacccg cgctgttccc
     2401 gcagtcaccg cgcgtgctgc acgcgcacat ccgcttccct gacagatttc aacagttttg
     2461 tgaataaccc tgtggatacc cgggcctctg cgcacgcaac cgcagaaacg atgcggcctt
     2521 caccattctg cactcactcc gatgcacttc t
//
LOCUS       DASZYN010000089         1718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_454249, whole genome shotgun sequence.
ACCESSION   DASZYN010000089 DASZYN010000000
VERSION     DASZYN010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454249"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /locus_tag="VHE33_02345"
     CDS             complement(<1..1337)
                     /locus_tag="VHE33_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVT96316.1"
                     /translation="MHGFKIGFLAVALSGSPVLAQTSNATSLTAAGTTMFKGGPALTG
                     VSTGSVPRAFEGIRFIVRTGGPIRSTPLLYRSGVYFGSSDGFLYAVDAHDGHPLWTLH
                     ASGPVASSPAARGDLIYFVARPNTLYAVTADRGEIRWQQDLGADKGPSNYWDFFESSP
                     TLVDDRLFVGSGDGRLYCIDAASGRRIWSFDAGARIRSTPAVVDNTVVVGAMNGRIYA
                     VDAATGKEKWSYATRGASHKFADKNNDTTSIVASPTIAGGLVAIGGRDGYLYAVDLQT
                     GKLRWDITHDGSSWILSTASQQGSLFIGSGSAFLLQAVDLSTGKESWRFKTHSAVFSS
                     PVIAGNVIVFSDLSGLVYAVDTMSGKELWSFNLPDRALSSPVVGDHTVYVASDDGKLL
                     ALDTGTTAHDAAVRRLVYWQGPLSAESFSWFPKGIDEAIRDAFVRSGFERIDRA"
BASE COUNT          376 a          503 c          538 g          301 t
ORIGIN      
        1 gcgcggtcga tgcgctcgaa gcccgagcgg acgaaagcat cgcgaatggc ctcatcgata
       61 cccttcggaa accaggaaaa cgactcagcc gacaacggcc cttgccagta caccagacgg
      121 cgcacagccg cgtcgtgtgc cgtcgtgccg gtatcaagcg cgagcagctt gccatcatcg
      181 gacgcgacgt acacggtgtg atctccgacg acaggcgacg agagggcccg gtcgggaagg
      241 ttgaagctcc acaactcctt gccgctcatg gtgtcgaccg cgtagaccag cccggagaga
      301 tcggaaaaca caatcacatt ccccgcgatg accggggaag aaaaaaccgc gctgtgcgtt
      361 ttgaaacgcc acgattcctt gcccgtggaa aggtcaacgg cctgcagcag gaaggcgctc
      421 ccgctcccga tgaacagact tccctgttgc gaagcggtgg aaaggatcca ggaggagcca
      481 tcgtgcgtga tgtcccagcg gagcttgccg gtctgcagat cgacggcgta gagatagcca
      541 tcgcggccgc cgatggccac cagcccaccg gcaatcgttg gcgaggcaac gatcgacgtc
      601 gtgtcgttgt ttttatcggc gaacttatgt gaagcgcctc gcgtggcgta gctccatttc
      661 tcttttccgg tcgcagcgtc gaccgcatag atccgcccat tcatcgcgcc aacgacaacg
      721 gtgttatcga caaccgcagg cgtggagcgg atgcgggcgc cggcgtcaaa gctccagatg
      781 cggcggcctg aagccgcatc gatgcagtag aggcggccat cgccgctgcc gacgaacagc
      841 cgatcgtcaa ccagcgtggg agaagattca aagaagtccc agtaattgct ggggcccttg
      901 tcggctccga gatcctgctg ccagcgaatt tccccgcgat cggcagtgac ggcgtacaga
      961 gtgttcggcc gcgcgacaaa ataaatcaaa tctcctctgg ctgccggaga agaggcaaca
     1021 gggcccgaag cgtggagtgt ccacagcggg tgaccgtcat gagcgtcgac ggcgtagaga
     1081 aacccgtcgc tgcttccgaa gtacacaccg ctccggtaga ggagcggtgt gctccggatg
     1141 ggtccgcccg tccgcacgat gaagcggata ccctcaaacg cgcgcggcac gcttcccgtg
     1201 gaaacgccgg taagggcagg tccgcctttg aacatcgttg tgccggcagc tgtgagggaa
     1261 gtcgcgttgc tcgtctgggc aaggaccggc gaaccggaga gtgccaccgc aagaaatccg
     1321 attttgaacc catgcatgct gtgtcagacc ccgggaaaac tcggcacatt ctactcacgc
     1381 accggcgatc gccggatcgc cgtctcggtt ttttgtgacc gaacgcgagc aaggtgctac
     1441 ggaacggcgt tttcggtggg cggctggccg aagcgctcga cgcagtttcg agggagaagg
     1501 gatcttgcag aggcgatggg atgttaaggc aaaaaagaaa agagccctcg gggctcctga
     1561 ccgaccgagg gctgtgaacg agcttcaccg gatcgtgcat gacagctcaa ttccgaacat
     1621 aagccctgga ggccaaatcc gtaccctatc gaaggtggga gattcgctct ttgttcgcct
     1681 atctttcgga ggcgtcgaag atggacaagt tgtaattt
//
LOCUS       DASZYN010000090        13392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_68678, whole genome shotgun sequence.
ACCESSION   DASZYN010000090 DASZYN010000000
VERSION     DASZYN010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 13392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13392
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68678"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(401..1054)
                     /locus_tag="VHE33_02350"
     CDS             complement(401..1054)
                     /locus_tag="VHE33_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVT96317.1"
                     /translation="MIPTVGILSTGPFDQSLSTLSSAIPPVQETENLRIAERLRAQDP
                     HVVDELILQYQHRLMRYLTFHTGSRELAEDLFQETWMRVLTRGAQFRGDSQFVTWLFS
                     IARNLVLDMRRKVPPSTSFEELTEMGDERWLNLFDRHSAFDHCASIEDARLLKRVFPS
                     LSADQRKVILLRFQQEMPLDQIAAATHAPVSTVKARLYRGLALLKTRMTALALRTSS"
     gene            1240..1674
                     /locus_tag="VHE33_02355"
     CDS             1240..1674
                     /locus_tag="VHE33_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96318.1"
                     /translation="MKFGWSVSFLVCSLIAASGVLVVSDSLMPAPPVLAASLPAQDAA
                     NNTSAAVDLSGTWQMSWTGQNGSQRQASMEIKQDGSKLSGSFEGQRGTAPLSGKLEGD
                     QVSLTVKIRRHDVSFSGTVNGDKMSGPTARGGTWTATRGQQQ"
     gene            1832..2593
                     /locus_tag="VHE33_02360"
     CDS             1832..2593
                     /locus_tag="VHE33_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT96319.1"
                     /translation="MRSPESTHALTAPPETAARHVLLIDDDRAMAEMLAEYMRPEGFI
                     VHLAHSAKAGLERAAEGGLVLIILDVMLPDQDGFAVLGALRQKSRIPIVMLTTRSSVT
                     DTVGGLEAGADDYVPKPFTPVELLARIRTVLRRGQPSRFGTPFLAIDDLILDAGSRTV
                     QRNGKLVDCTAAEFDALHTLASSAGEVVTREHLSRMALGRSPYAGDRGIDNLVSSLRK
                     KLGPFSNGRERLRSVRNTGYVYLHCASTAPREQDR"
     gene            2590..4014
                     /locus_tag="VHE33_02365"
     CDS             2590..4014
                     /locus_tag="VHE33_02365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT96320.1"
                     /translation="MRLFLRIFLSFWIATVLMIVVMASATELVPMDLHIDRGPGFEPD
                     VTRAALTNLVQQTDIQDRTATDAALRNLAGGHRHKIFLFDENGNIIAGEGKPPLLYSH
                     LAREVAGQGHARLQRFLGLRMLYACPVVGLGGKRYAAVMTVFEPGERLLRSRFWINMT
                     LAMVPSGLVCLLLALYITRPITRLRSTAQRLAGGDLSARSSERRSERTDELGDLARDF
                     DTMAAQIERLMTAQRRFVADVSHELGAPLTRLHLALALLRRRQDGATTPELQRIERET
                     DRLSNLVQQLLLLAGLEAGRVPAETLSSISLRSLCDSIVEDANFEAEQAGCCVAGTRA
                     DATFLAYPQMLRRAIDNILRNAIRYTAPGSEVRLECSIDEAQQQVVIEVTDRGPGVPE
                     SMLSDIFLPFFRTSPGRESDTGGTGLGLAIASEAVQLHEGSIVARNREDGGLRVTITL
                     PFRTPTADEGTNPAQAEPLIPIEK"
     gene            complement(4120..5487)
                     /locus_tag="VHE33_02370"
     CDS             complement(4120..5487)
                     /locus_tag="VHE33_02370"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HVT96321.1"
                     /translation="MSIKNRNDHSLVASASLPAGSFRNIAHAAAQTLTQCLRIGTAVL
                     LIAFMVPLGIVDAHAQVPAPQGSYQQLSNEQVNQLVAPIALYPDALVAQVLGAATYSP
                     QVVEADRFVKEHAGIPPQQLAQMADTQPWDPGVKALVAFPTVLANMSRNLDWTRRLGN
                     AYYNQPQDVMNAVQQMRQRAYAAGTLRTTAEQTVVYRPADIVIAPANPAVVYVPAYNP
                     WAVYGAPVPVYPAYYYVAPPPRPGAVVAAAAVGFTIGVVVGAFAHYGWGWGHWGCGWG
                     PHPVVAFNHVTYVSRSVTVINHGYYGRFDHSAEARSWNQHVAVGPRGGVYTDTRSMGN
                     GEYSNTRSATGPRGANYTDTRTAGDGQYSNTRTATGPHGANYTDQRTAENGALSNTRT
                     VTGPNGKTASSTTDRYAGGSTRTATGPNGKTATRTVSGRGTGDATITRSGPRGTRTRN
                     RVRPH"
     gene            complement(5739..6296)
                     /locus_tag="VHE33_02375"
     CDS             complement(5739..6296)
                     /locus_tag="VHE33_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HVT96322.1"
                     /translation="MEAARRIFARDGFEMTRLDDIAAAAGRTRGAFYANFRDKEDVFF
                     ALFEENLRREKRHMGEQLSEAGSEDERIEVLAQHMLSTIKDRRRMLLAIEFKLYAIRH
                     PKTTKRLADLHAALCLSCVEANVEKLIPGFSCLTAHQKRLQTAQVGAMLDGIALNLMF
                     DSRGLTQELILRHLRSALRIALQQR"
     gene            6500..7747
                     /locus_tag="VHE33_02380"
     CDS             6500..7747
                     /locus_tag="VHE33_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVT96323.1"
                     /translation="MTAKQNPRLNHRQRFLAAIALPVWLVVSAAALSGCQSGSAATAG
                     QGMGAMPVSVVRVAHADVPVNGSWVGTLDGYVNAQIQPQVSGYLVKQNYREGAPVTRG
                     ELLFQIDPRPFQAAVDQAQAQVEQAKGQLAQAHAQLALAEINVKRDTPLAKVRAIAQS
                     QLDTETQQEAQAQATVAAGEASVSAAQAALKTAQLNLGFTQVRSLIDGVAGQATTQVG
                     NLVSPQSVLTAVSQLDPIKVYFSISDGEYLALVDRAKQSGGDLLRGGSNLPLTLTLAN
                     GETYPHKGRIIFVDRQMNQQTGAIRIAASFPNPGNVLRPGQFGRVTAATEIRHGALLV
                     PQIAVQELQGIEQIYTVGPGNHVHVVNVKLGPQYGSDWIVESGLAPEAQVITDNLQKL
                     KEGVPVAPHAAQLPAANNPTAGS"
     gene            7751..10954
                     /locus_tag="VHE33_02385"
     CDS             7751..10954
                     /locus_tag="VHE33_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HVT96324.1"
                     /translation="MSKFFIRRPIVAIVIAILTVIIGTVSMLSLPTSQYPNIVPPEIQ
                     AQATYPGADAKTLTEAVATPIEQQMNGVDNMLYMYSVNANNGQSTVYVDFDVKSNADT
                     DQVLSQLRVSQAQSQLPAQVNTAGVTVIKSLTSPLMLIGLSSPDNRYGQEFLTNYAII
                     NLQDELTRVPGVSRVQVFGGQYALRVWVRPDQLAKLGVTATDVINAIQTQNNVNPAGQ
                     IGGEPIPSGQQFTYTVRTQGRLVTPEEFGNIIIRANPDGSVLHLHDVARVEMGEQIYS
                     LSARYNNAPAGMMAVYQLPGSNAVETARLVTKRIKELSANFPAGMRYDIPLDTTKAVT
                     AGIREIVITLGIALLLVVLVVYLFLQSWRATLIPLMAVPVSLIGTFIIFPALGFSINT
                     LSLFGLVLAIGLVVDDAIVVVEAVEHHIEEGMTPRDATIRAMEEVGSPVVAIALILAA
                     VFIPTAFIPGITGKLYQQFAVTIAISVLISAFNALTLSPALAAMLLKPREHKIRRSPL
                     QLFFDRFNFFFSRTTDKYVSTSSRLIHKSAITMIVLGVIAVVAVFMGGRLPSGFLPQE
                     DQGYLFAALVLPDASSMQRTDEAVQKVTNTLLHTPGVGGVVGVDGFNLLTLTQSTNTG
                     FFFISLKPWEMRKSRQEQIESIQANVQKQLMGLGDGLAFSFPPPQIPGVGTSGGVTMI
                     LQDRSGKDDPSFLTKNLFGFLGALSKRPEIAAAIPSYMPAVPQLYADVDREKAEQQQV
                     DLNNVYTTMQTFMGGYLVNYFNRFGRQWQTYVEAEGDTRSNIQNIGEFYVRSANGSQV
                     PLSSLVHVKQITGPEFIMRFNEYNAAQLNITGAPGYSSGQVRAALEETFHQTMPPGMG
                     FSYSGMSFQEQRAAEGTPAWVVFALSLLFVFLILAALYESWTLPFSVLLSTPVAILGA
                     YLALTSRSLENDIFATVGLIMLIGLSAKNAILIVEFAVQDYRTGRSICDSALRAARLR
                     FRPIVMTALAFIVGCIPLWTASGAGAASRRILGTVVIGGMSLATVIGILMIPTTFSVV
                     EYLSHRFARGGKGTTMDSTRGICDPEPVAVEAEGDAA"
     gene            10951..12378
                     /locus_tag="VHE33_02390"
     CDS             10951..12378
                     /locus_tag="VHE33_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HVT96325.1"
                     /translation="MTSSSQHLSPGWKIALGALFLVLLAGCTVGPNYKRPQVPTAPAF
                     RGADGVDAATAGNASLGDENWADVFREPELQGLIRTALSNNYDVRIAAQRVLEQQAQV
                     KITRSQQFPTLSVGGTGIGAELPDSLSKTLGGSTLSAGSFNLSAAWTPDFWGLYRRQT
                     EAARAQMLSEVWAQRAVRMTLVEQVASAYIQLRALDRQLEIAKQTLETRRQSVDLTRR
                     LADAGSAPLSDLRQAEELEYTASSQIPQLEQQIQQQENALRLLLGENPGRVAHSDDKA
                     LAPPPQDLPVGLPSRLLERRPDILAAEQQLVAANARIGVARAQFFPQLSISASGGVGG
                     DEWSNLFDPSGHTIYGIGSLTQPIFQGGRLRGQLQLSEHAKEEMVLNYQKTISGAFRD
                     VSDALIALNKQRAAREQQTLLVAAAEDATRLAKTRYQGGATSYLEVLTTDSNLFSAQL
                     NLVNAQEGEALSLVQLYGALGGGWN"
     gene            complement(12413..>13392)
                     /locus_tag="VHE33_02395"
     CDS             complement(12413..>13392)
                     /locus_tag="VHE33_02395"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HVT96326.1"
                     /translation="RHDGRGIETSGRRAGRMGNEPRMNQAPRVRIGIDWGGTKIEALA
                     MDAEGRELRRVRIPTPRHDYEGSIRAAAELVASIEREVGPSGPIGVGIPGSIVPTTGL
                     VKNANSTWLNGRPLEKDLSLALGREVRCVNDANCLAVSEAIDGAAAGHAVVFAAILGT
                     GCGAGIALHGRMHTGPNGLAGEWGHNPLPWQRPDEYPGDLCYCGQRGCIERWLSGPAL
                     EEDYLRAAGSALSGLEIVAASEAGDPQAIVALERYEDRLARSLAHVVNLLDPDIIVLG
                     GGLSRIERLYRNVPSRIATSAFGKSAATPVVPAKHGDSSGVRGAARLWS"
BASE COUNT         2474 a         4043 c         4239 g         2636 t
ORIGIN      
        1 gctttgagca gccgccccca gcgaactgcg tcgccctcgc cggagcgcgt gacggcctga
       61 tgataggtgg gatcgtaaaa ctgcatcatc tgctcgggag ggggcggatc gaacagccac
      121 acgtgcgaac atgaagggca acgaagcagt gtgtagctcg gctcagaggc cgacagaggg
      181 cgtgtgcgca tccagggcgc attcgtacgc tgtgcacagc aagggcatgg gcccgtggct
      241 tcgcggttga tctccgcctg ggaattcatt gcaagtattt tctcgtgtct ggacggtacg
      301 tgggcatgac catttcagtc cgagcggatc cggcagtccg gagggaaggg gttcgttcgg
      361 tccggcgaga gtttttgaag cagcacatga ccgtcgcctg ttaagaactg gtccggagtg
      421 caagggcggt catccgcgtt ttgagcaggg ctaatcctcg atacagcctt gctttcacgg
      481 tcgagacggg cgcgtgcgtg gcagctgcaa tctgatcgag gggcatttcc tgctgaaaac
      541 ggagcagaat gactttgcgc tggtcggcag agagcgaagg gaatacgcgc ttcaggagcc
      601 gcgcatcttc gatcgacgcg cagtggtcga acgcgctgtg gcggtcgaag aggttcagcc
      661 agcgctcgtc tcccatctct gtcaattcct cgaaactggt ggagggcgga accttccgcc
      721 tcatatcaag gacgagattg cgcgcgatgc tgaaaagcca ggtaacgaac tgcgagtctc
      781 ctctgaactg agcgccgcga gtgagaacgc gcatccaggt ttcctgaaac aggtcttcgg
      841 ccagttcgcg gctgccggta tgaaaggtca ggtaccgcat aagacggtgt tgatactgca
      901 ggatgagctc gtcgactacg tgcggatcct gcgcgcgcag cctttcggcg atacggaggt
      961 tctcggtttc ttgtacgggc ggtatcgcac tggaaagggt gctcaaggat tgatcgaaag
     1021 gtccggtact aagaatgccg acagtgggga tcatttggtt gtgctccagg gtgatggacg
     1081 ggtttgtgcg cggaaagaag tgatggcaat gtcgggaatt gtcaatacgg aatcgatcgc
     1141 tgcgagcgct cgacaaaact cgacaaaacc ttcgcgaccg gcgaggcaaa gacggcgtct
     1201 gaggctgaga aagcgccaat tcccctctgg aggttccctg tgaagttcgg ttggtcagtt
     1261 tcttttctgg tctgctccct gatcgcggcg agcggtgtcc tcgtagtctc cgattcgctg
     1321 atgccggctc cccccgtgct ggctgcgtcg ttgcccgcac aggatgccgc gaacaacacg
     1381 tcggctgcgg tcgacctttc cggcacctgg cagatgtcct ggacggggca gaacggcagc
     1441 cagcgccagg cttcgatgga gatcaagcag gacggcagca agctaagcgg cagcttcgaa
     1501 ggccagcgcg ggacggctcc tctctcaggc aaactcgaag gcgaccaggt ttcgctgacg
     1561 gttaagatcc ggcgtcacga cgtatctttc tccggcacgg tgaacgggga caagatgagc
     1621 ggtccgacgg cgcgcggcgg cacctggacg gccacccgcg gtcaacagca ataagccatg
     1681 gcgcctccca tctccgaccg cgagtcgtgc gtcgcggcaa tgccgactcg cggtgccggg
     1741 ccgtgcatcg accatctttt cgccgcgagt cttgccgcca gcgatggttg acggacgcgc
     1801 cgactgcgac gcacccttaa aggttgcggt catgcgctcc cccgaatcga ctcacgccct
     1861 gacggccccg cctgagaccg ccgcgcgtca tgtcctgctc atcgacgacg accgcgctat
     1921 ggccgaaatg ctcgcggaat acatgcgacc ggagggcttt atcgtgcacc tggcgcactc
     1981 ggccaaagca ggtctggagc gagcggccga aggcggcctg gtgctgatca ttcttgacgt
     2041 tatgcttccg gatcaggacg gattcgcggt gctgggcgca ctgcggcaga agagccggat
     2101 tccgattgtc atgctgacca cgcgctcttc cgttacagat acggtaggtg gtctggaggc
     2161 cggcgccgac gattacgtcc ccaagccttt cactccggtc gaactgctcg cgaggatccg
     2221 aacggttctg cggcgcgggc agccctcgcg attcggcaca ccttttcttg ctattgacga
     2281 tctgatcctt gatgcgggat cgcgcaccgt tcagcgcaac ggcaaactgg tggactgcac
     2341 tgccgccgaa ttcgacgccc tgcacacgct ggcttcgtca gcaggagaag tggtcacacg
     2401 ggagcatctc tcccgcatgg cgctgggccg ttcgccctat gcgggggatc ggggcatcga
     2461 caatctggtc agttctctgc gcaaaaaact cggccccttc agcaatgggc gcgaacgact
     2521 gcgctcggta cggaataccg ggtacgttta cctgcactgc gcatcgactg caccgcggga
     2581 acaggaccga tgagactctt cctgcgcatc ttcctcagtt tctggattgc caccgtgctc
     2641 atgatcgtgg tgatggcgag cgcgactgaa ctggtgccca tggatctgca tatcgaccgc
     2701 ggtcccgggt ttgaaccgga cgtgacgcgg gcggcgctga cgaatctggt ccaacagacg
     2761 gatattcagg accggaccgc gaccgatgca gctctccgca atttggccgg ggggcatcgt
     2821 cacaagattt ttctcttcga tgagaacggc aacatcatcg ccggtgaagg gaaaccgccg
     2881 ctgctttact cgcacctcgc ccgggaagtg gccgggcagg gacatgctcg tctgcagcgg
     2941 tttctcggtc tgcggatgct ctatgcgtgc ccggttgtgg gcctgggcgg gaagcgttac
     3001 gcggcggtga tgacggtctt cgagccggga gaacgtcttc tgcgctcgcg cttctggatc
     3061 aacatgacac tggcgatggt tccttcaggg ctggtgtgcc tgctgctggc gctttacatc
     3121 acgcgtccga ttacacgatt gcgttcgaca gcacagcgcc ttgccggcgg cgatctgagc
     3181 gcgcggtctt cagagcgacg aagcgaacgt acggatgaac tgggcgatct ggcgcgcgat
     3241 ttcgacacga tggcggcaca gattgagcgg ctgatgaccg cgcaacgccg gtttgtggcc
     3301 gatgtttctc atgagttggg tgcgccgctg acgcgtttgc atctggcgct ggcactgcta
     3361 cggcggcgcc aggacggagc caccacgccg gaactgcagc gcatcgagcg cgagaccgat
     3421 cgcctcagca acctcgtcca gcagctgctt ttgctcgcgg gactggaagc gggccgagtg
     3481 ccggctgaga cgctgagctc catttcgctg cgatcgctct gcgacagcat cgtcgaagac
     3541 gcgaacttcg aagccgagca ggccggctgc tgtgttgcgg gaacgcgcgc ggatgcgaca
     3601 tttctggcct atccgcaaat gctgcgccgc gcgatcgaca acatcctgcg caacgcgatt
     3661 cgctacactg cgccggggtc tgaagtgcga ctcgaatgca gcatcgatga ggcgcagcag
     3721 caggtcgtta tcgaagtgac ggaccgcggc ccgggtgtgc cggagtcgat gctctcggat
     3781 atcttcctgc ccttcttccg tacctcaccg gggcgagaaa gcgatacggg tgggacggga
     3841 ctgggtttgg cgattgcctc ggaggcggta cagctgcacg aaggctcgat cgttgcacgg
     3901 aatcgcgaag atggcgggtt acgggtgacg atcactctgc ccttccgaac gcctacagcg
     3961 gatgagggaa cgaatcctgc gcaggcagag ccccttatcc cgatcgaaaa ataagctggt
     4021 gcgctgagcc ggactttggg gcgagccagg gagagttctc tgctcaaaag gcagcggcca
     4081 ccgaaggcct cgatggccgc tgcccaaaag cgcaacgggc tagtgtggcc gtacgcgatt
     4141 ccgggtcctg gttccgcgcg gtccggaacg tgtgatggtc gcatcgccgg tgcctcgacc
     4201 gcttaccgtg cgggtggctg tttttccgtt gggaccggtt gcggttctgg tgcttccacc
     4261 cgcatagcga tccgtcgtgc tggacgccgt tttgccattc gggccggtga ccgttcgcgt
     4321 gttgctgagg gcgccatttt cggcggtgcg ctgatcggtg tagttcgccc catgcggccc
     4381 ggtcgccgta cgcgtgttgc tgtattggcc gtcgccggcg gtgcgggtat cggtgtaatt
     4441 cgccccgcgg gggccggtgg cggaacgcgt gttgctgtac tcaccattgc ccatcgaacg
     4501 ggtgtcggta tacacgccgc cgcgcgggcc gaccgccaca tgctggttcc agctgcgcgc
     4561 ttcagcggag tgatcgaacc tgccatagta gccgtgattg atgaccgtca cgctgcgcga
     4621 gacgtacgtc acatggttga aggccaccac gggatgcgga ccccatccgc agccccagtg
     4681 gccccatccc catccgtagt gagcaaaggc gccgactaca acgccgatcg tgaatccgac
     4741 cgcagccgcg gccacgaccg ctccgggtcg cggtggcggc gcgacatagt agtaagccgg
     4801 atacacaggc accggcgctc cgtaaacggc ccacgggttg taagcgggca cgtagaccac
     4861 agcagggttc gccggagcaa tcacaatgtc cgcgggacga tagaccaccg tctgctcggc
     4921 tgtggtgcgg agagttccgg ctgcgtaggc gcgctgccgc atctgctgga cggcgttcat
     4981 cacgtcctgc ggctggttgt aatacgcatt gcccagacgt ctggtccagt ccaggttgcg
     5041 gctcatgttt gccaggacgg tgggaaaggc aaccagcgcc ttcacgcctg ggtcccaggg
     5101 ctgagtatcg gccatctgtg ccagttgctg tggaggtata cccgcatgtt ccttcacgaa
     5161 gcggtcggcc tccacgacct gtggagaata cgtcgccgcg cccagaacct gcgcgacgag
     5221 ggcatcggga tagagcgcga tcggcgcaac gagctggttc acctgctcgt tactcagctg
     5281 ttgatagctg ccctggggcg cgggcacctg cgcatgcgcg tcaacaattc ccaggggcac
     5341 catgaacgcg atcaggagca cggccgttcc aattcgcagg cattgcgtca gggtttgggc
     5401 ggcagcgtgg gcgatgtttc ggaagctgcc agccggcagc gatgcagaag caacaagact
     5461 gtggtcgttt cgatttttga tactcatgtt tgccttcgga tctgaattcg gcgcaatggg
     5521 gaactgagct tcgcttcact cctggggtcg tcattgcacc cattcagtgt gaccctccgg
     5581 cagcgcaagt tatgtcgggt tttgtcgcct gtgcctcggg tgctcgcctg cgaaacagcg
     5641 atgcagcaca atgattcgaa gtaatgggac ttcactcggc gcaagggcgg acgaaggcgg
     5701 gctttccgga agaaaaatcg attgcgccgg cgaaaggtct atcgctgctg cagtgcgatg
     5761 cgcagtgcgg accgcagatg ccgcaggatc aattcctgcg tcagcccgcg gctgtcgaac
     5821 atcaggttca gcgcaattcc gtcgagcatc gccccgacct gggccgtttg caggcgcttc
     5881 tggtgtgcgg tcagacagct gaacccgggg atgagttttt cgacattggc ctcgacacaa
     5941 ctcaggcaga gggccgcatg caggtccgcg aggcgcttcg ttgttttggg atggcggatc
     6001 gcatagagct tgaactcgat ggccagcagc atacggcggc ggtccttgat cgtcgagagc
     6061 atgtgctggg ccaggacttc gatccgttcg tcttctgacc cggcttcgct gagctgttcg
     6121 cccatgtgcc gcttctcgcg gcgcaggttc tcctcaaaca acgcgaagaa cacgtcctct
     6181 ttgtcacgaa aattggcata aaacgcgccg cgcgttcttc ccgcggcggc ggcaatgtcg
     6241 tccaggcggg tcatttcaaa gccatcgcgg gcgaaaatgc ggcgcgccgc ctccatcagt
     6301 tcgcggcgcg tggaaatggc acgatcctgc tggcctgttt tcctcacgtt gcttcggatg
     6361 ctcgagcgcc cgccaggctt cgtgatcatt tttgcgtgga cctccgtcac aattttgcat
     6421 cgggaggggg gactgccgaa tctctgacca atatacatcc gtgtatgttt ttcgcaaccc
     6481 attcacgcag gagtaactca tgacggcgaa acaaaaccca cggttgaacc atcgccagag
     6541 atttttggcg gctattgcgc tgcctgtctg gctcgtggtc tcggctgcag cgctcagcgg
     6601 ctgccagtcg ggaagcgcgg ccacggccgg gcagggcatg ggagccatgc cggtttccgt
     6661 tgtgcgcgtt gcgcacgccg atgtgccggt gaacggatcc tgggtcggga ccctggatgg
     6721 ctatgtgaac gcgcagattc agcctcaggt cagcggctat ctggtcaagc agaattaccg
     6781 cgaaggcgcg cccgtcacca ggggcgagct gctcttccag atcgatccgc gcccgtttca
     6841 ggcggccgtc gatcaggcgc aggctcaggt agagcaggcc aagggacagc tggcgcaggc
     6901 ccacgctcaa ctcgctctcg cggagatcaa cgtaaagcgc gacacgccgc tggcgaaggt
     6961 gcgggccatt gcgcaaagcc agctcgacac cgagacgcag caggaagccc aggcacaggc
     7021 cacagtggct gcgggagagg cgtcggtgag cgcggcgcaa gcggcactga aaaccgcgca
     7081 gctgaacctg ggctttactc aggttcgatc gctgatcgat ggcgtagccg gccaggcaac
     7141 gacgcaggtt ggcaacctgg tgagtccgca gtccgttctg acggcggttt cgcagctcga
     7201 tcctattaag gtctacttct cgatcagcga cggcgaatac ctggcgctgg tggatcgggc
     7261 aaagcagtcg ggaggcgacc tgctgcgtgg gggatcgaat ctgccgctca cgctaaccct
     7321 ggccaacggc gagacgtacc cgcacaaggg gcgcatcatc tttgtcgacc ggcagatgaa
     7381 ccagcagacg ggtgcgattc gcatcgctgc ttcgtttccg aatccgggta atgtgctgcg
     7441 gccaggtcaa ttcgggcgcg tgacggcagc cacggagatt cgccacggtg cgctgctggt
     7501 tccgcagatc gcagtgcagg agctgcaggg aattgagcag atttacaccg tcggccccgg
     7561 caatcatgtg cacgtcgtga atgtgaagct cgggccgcag tacggcagcg actggatcgt
     7621 ggagagcggt ctcgcacctg aggcccaggt catcaccgac aacctgcaaa agctgaaaga
     7681 gggagttccg gtcgcgccac acgcggccca gcttcccgca gcgaataacc ccacagcagg
     7741 gagctaacgc atgtcgaagt tttttatccg gcggcccatc gtcgcgatcg tcatcgccat
     7801 cctcaccgtg atcattggca cggtatcgat gctgagcctg ccgacgtcgc agtaccccaa
     7861 catcgtgcct ccggagattc aggcgcaggc cacttatccg ggcgccgatg cgaagacgct
     7921 gaccgaagcc gtcgccacgc cgatcgagca gcagatgaac ggcgtcgaca acatgctgta
     7981 catgtattcg gtcaacgcca acaacgggca gtccactgtc tacgttgact tcgacgtcaa
     8041 aagcaacgcc gacaccgatc aggtgctgtc gcagctgcgc gtctcccagg ctcagtctca
     8101 gctgcccgct caggtgaaca cggctggcgt cacggtgatc aagtcgctca cctcgccgct
     8161 gatgctcatc ggactttcgt cgccggacaa tcgctacggc caggaattcc tcaccaacta
     8221 cgcgatcatc aatctgcagg atgagctgac gcgcgtgccc ggtgtatcgc gagtgcaggt
     8281 cttcggcggc cagtacgctc tgcgtgtgtg ggtgaggccc gatcagctgg ccaagcttgg
     8341 cgtgacggcg actgacgtta ttaacgcaat ccagactcag aacaacgtca atccggcggg
     8401 ccagatcggc ggcgaaccca taccttcggg ccaacagttc acctatacgg tgcggaccca
     8461 gggccggctc gtgactcctg aggaattcgg caacatcatc attcgtgcca atcccgacgg
     8521 ctcggtgctg cacctgcacg atgtagcccg cgtcgaaatg ggcgaacaga tctacagcct
     8581 ctcagcgcgc tataacaatg cgcctgccgg catgatggcg gtctaccagc tgccgggctc
     8641 caacgccgtg gaaacggcgc gcctggtcac aaaacggatc aaggaattgt cggcgaactt
     8701 ccccgccggg atgcgttacg acattccgct ggacacgacc aaggcggtta ctgccggcat
     8761 tcgcgaaatc gtcatcacgc tcggtatcgc gctgttgctc gtcgtgctcg tcgtttatct
     8821 cttcctgcaa agctggcggg cgacactgat tcccctgatg gcggtgcctg tatcgctgat
     8881 cggcactttc attatctttc cggctctcgg cttctccatc aacacgcttt ccctcttcgg
     8941 cctggtgctc gccatcggcc tggtggtgga tgacgctatt gtcgtggtgg aggcagttga
     9001 gcaccacatt gaagaaggca tgactccgcg cgacgcaacc atccgcgcga tggaggaagt
     9061 ggggtcgccg gttgtggcca ttgcgctcat ccttgccgcc gtatttatcc cgacggcctt
     9121 catccctggt atcaccggca agctctacca gcagttcgcc gtcaccatcg cgatctctgt
     9181 cctcatctcg gctttcaacg cgctcacgct cagtccggca ctggccgcga tgctgcttaa
     9241 gccccgcgaa cacaaaatcc gccgcagccc gctgcagctt ttcttcgatc gcttcaactt
     9301 cttcttcagc cgcacgaccg acaaatatgt cagcacatcc agccgcctga ttcacaagtc
     9361 ggccatcacc atgatcgtgc tgggtgtcat cgcggtggtt gccgtcttta tgggaggccg
     9421 cctgccttcc ggcttccttc ctcaggagga tcagggctac cttttcgccg cgctggtgct
     9481 gcccgacgca tcttccatgc agcggaccga tgaggccgtt cagaaagtga ctaacacgct
     9541 gctgcacaca cccggcgtag gtggtgtggt tggcgtcgac ggattcaacc tgctcacgct
     9601 gacccagagc acgaacactg gattcttctt catttcactc aagccgtggg agatgcggaa
     9661 gtcgagacag gaacagatcg agagcatcca ggccaacgtg cagaagcaac tgatggggct
     9721 tggcgatgga ctcgcgttct cgttcccgcc accgcagatt ccaggtgtgg gcacatcggg
     9781 cggggtgacg atgatcctgc aggatcgctc cggaaaggac gacccctcct tcctcacgaa
     9841 gaaccttttc ggattcctgg gagcgctgtc gaagcggcct gagatcgcgg cggcgattcc
     9901 ctcgtacatg ccggcggtgc cgcagttgta tgcggacgtt gatcgcgaga aggcagagca
     9961 gcagcaggtg gatctgaaca atgtgtatac gacgatgcag acgttcatgg gcgggtatct
    10021 ggtgaactac ttcaaccgct tcggacggca atggcaaacc tatgttgaag ccgagggcga
    10081 cacccgcagc aacattcaga acatcggcga gttctatgtg cgcagcgcga acggaagcca
    10141 ggtgccactc agctcgctgg tgcatgttaa gcagatcacg ggtccggagt tcatcatgcg
    10201 cttcaacgaa tacaacgcgg cgcagctgaa catcacaggc gctcccggct acagttccgg
    10261 ccaggtgcgc gctgcgctgg aagagacttt tcaccagacg atgccgccgg gaatgggttt
    10321 ctcttattcc ggcatgagct tccaggagca acgcgcagcc gagggcaccc cggcgtgggt
    10381 ggttttcgca ctgtcgttgc tgttcgtgtt cctcattctc gccgcgctgt atgaaagctg
    10441 gacactcccc ttcagcgtcc tgctcagcac gccggtggca attctcggcg cctatctcgc
    10501 actgacgtcg cgttcgcttg agaacgacat cttcgccacg gtcggactga tcatgctcat
    10561 cggcctttcg gcgaagaacg ccatccttat cgtcgagttt gcggtgcagg actatcggac
    10621 cggccgaagc atctgcgatt ccgctctcag agcggcacgc ctgcgctttc gtcccattgt
    10681 tatgactgcg ctcgcgttca tcgtcggctg cattcctctg tggaccgcca gcggcgcggg
    10741 ggctgcgtcg cgccgcattc ttggcaccgt ggtgattggc ggcatgtcac tcgcgacggt
    10801 gatcggaatc ctcatgattc ccacgacctt ctccgtcgtg gaatacctgt cgcatcggtt
    10861 cgcgcggggg ggcaagggaa ccacgatgga ttcaacccgt ggcatttgcg accccgagcc
    10921 ggtagcggtg gaggcagaag gagacgccgc atgacctcat cgtcgcaaca tctttctccg
    10981 ggctggaaaa ttgcgctggg cgctttgttc ctcgttcttc tcgcgggatg cactgtcggt
    11041 ccgaactaca aacgaccgca ggtacccaca gcgcctgcgt tccgcggagc ggatggcgtc
    11101 gacgctgcga ccgccggcaa cgcatcgctc ggcgacgaga actgggctga cgtgtttcgc
    11161 gagcctgagt tgcaggggct gatccgaacc gcgctgagca acaattacga tgtccgcatc
    11221 gcggcccagc gcgtgctcga acagcaggct caggtaaaga tcacccgctc gcagcagttc
    11281 cccacactgt cagtgggcgg caccggtatt ggcgcagaac tgcccgactc gctcagcaag
    11341 accctgggtg gaagcactct gtcagccggc agcttcaatc tctccgcagc ctggacgccg
    11401 gacttttggg ggctctatcg ccgccagacg gaagccgcgc gagcgcagat gctgtctgag
    11461 gtctgggcgc agcgtgctgt gcgcatgacg ctggtcgagc aggtcgcttc ggcatatatc
    11521 caattgcgcg cgctggaccg ccagctggaa atcgcaaagc aaacgctcga gacgcgtcgt
    11581 cagtccgtgg atttgacgcg ccgcctcgcc gacgccggtt cggcgccgct ttccgatctg
    11641 cgccaggccg aggaactgga gtacacggca tcatcgcaga ttccgcagct ggagcagcaa
    11701 atacagcaac aggagaatgc gcttcgcttg ttgctgggcg agaatccggg ccgggtcgcg
    11761 cacagcgacg acaaggccct ggcgcctcct cctcaggatc tgcccgtcgg attgccttcg
    11821 cgtctgctcg agcgcaggcc cgacattctt gcagcggaac agcaactggt ggcggccaat
    11881 gcgcggattg gcgtcgcccg ggctcagttc ttcccgcagc tctccatcag cgcatcgggt
    11941 ggagtcggcg gagacgagtg gtcgaatctg ttcgatccgt ccggccatac catttacggc
    12001 attggcagcc tcacccagcc gatttttcag ggcggcaggc tcaggggcca gcttcagctc
    12061 tccgaacacg ccaaagagga gatggtgctg aattatcaga agaccatcag tggggcgttt
    12121 cgcgatgttt ccgatgcgct catcgcgctc aataagcagc gtgcggcgcg cgaacagcaa
    12181 acgttgctgg tggccgcggc tgaggatgcg acgcggctgg cgaaaacccg ctatcagggt
    12241 ggcgctacca gttatctgga agtgctcacc accgattcca atcttttttc tgcgcagctg
    12301 aacctcgtca acgcgcagga gggagaagcg ctctcgctgg tgcagctgta tggcgcattg
    12361 ggcgggggct ggaattaagt gtttccggat tgaggttctt tgcgccgggc gattatgacc
    12421 acaggcgggc tgcaccgcgc acaccgctgg aatcgccatg cttcgcggga acgacgggag
    12481 tcgctgcgga tttgccgaac gccgatgtcg cgattcgtga aggcacgttg cgatagagac
    12541 gctcgatgcg ggagagccct ccgcccagca cgatgatgtc cgggtccagc aagttcacca
    12601 cgtgcgcgag cgagcgtgcc agacgatctt cgtaacgctc cagtgcaacg atcgcctgcg
    12661 gatcgcctgc ttcgcttgcg gcaacgattt cgagtccaga cagggccgat cctgcggcac
    12721 ggagatagtc ctcttccagc gccggaccgg agagccatcg ttcaatgcag ccgcgctgac
    12781 cgcaatagca aagatccccg ggatactcgt cggggcgttg ccagggcagc ggattgtggc
    12841 cccattcgcc ggcgaggccg ttgggaccgg tgtgcatccg gccatgcagg gcgatacccg
    12901 cgccacagcc tgtgccgaga atagctgcga agacgacagc gtggccggcc gcggctccgt
    12961 cgatggcctc agagacggcg aggcaattgg cgtcgttaac gcagcggacc tcgcggccca
    13021 gggcaagaga caaatccttt tcgagcggtc ttccgttgag ccaggtggaa ttcgcattct
    13081 tcacgagacc tgtcgtgggc acgatggagc cgggaatgcc gacgccaatg gggccagagg
    13141 gtccgacttc acgctcgatg gatgcgacca gctctgcagc ggcgcggata gagccttcgt
    13201 aatcatggcg tggtgtgggg atgcgcacgc gacgcagctc gcgtccctcg gcgtccatcg
    13261 cgagtgcttc aattttggtt ccgccccagt cgatgccgat gcgtacgcga ggcgcctgat
    13321 tcatgcgcgg ttcgttcccc attcgtccag cgcggcggcc agacgtttca atgcctcggc
    13381 cgtcatggcg gt
//
LOCUS       DASZYN010000091         7330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1032133, whole genome shotgun sequence.
ACCESSION   DASZYN010000091 DASZYN010000000
VERSION     DASZYN010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7330
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032133"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(300..2204)
                     /gene="uvrC"
                     /locus_tag="VHE33_02400"
     CDS             complement(300..2204)
                     /gene="uvrC"
                     /locus_tag="VHE33_02400"
                     /inference="COORDINATES: protein motif:HMM:NF011263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HVT96327.1"
                     /translation="MDLHEKIRTLPTRPGVYLYKNADGEVIYVGKARNLRSRVRSYLL
                     DAAQANAKTGSLMRDAVDVDYILVDNEHEALALENNLIKQRKPRFNILLRDDKTYPYV
                     KLTLGDRWPKVFVTRRLRRDGSAYYGPYFPGNLAYRVVDLIHRSFLLPSCKIDLSRYH
                     SRPCLQYYIGRCLGPCVQDLTTPEAYAEAVRDAQMFLEGRTAELNKSLRARMASAAAA
                     EQYELAAKYRDLLVTIDQLSERQRIASVEDDDADVFGFHSESGMIAVNLFHMRGGKIV
                     DRREFFWEDLPDFVGELPESESGPVAVEQAEATFEPGAFFSALLKQLYIDQQYVPKSI
                     YLPVDFADRSSLASLLAEHTKHRVEIAVPQRGDKRSLVDLAGQNAKQSFDQRFRVLAP
                     SRKAIQEALQDALTLEDLPRTIECFDISHIQGAETVASMVVWEDGEMKKSAYRKFRIK
                     TVEGVDDFASMREVVTRRYKRIVEEKQKMPSLILIDGGLGQLHAAAEALESLGITTQP
                     LASIAKREEIIYLYGQEDEPVVLDRRSPVLHLVQRIRDESHRFAIAYHRKRREMRDRD
                     SELLTIPGVGARTRQRLLEHFGSLRSIAVANLESLAAVVPPKTAEEIHAYFHAAETPT
                     TSLPVLNDSR"
     gene            2488..3246
                     /locus_tag="VHE33_02405"
     CDS             2488..3246
                     /locus_tag="VHE33_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HVT96328.1"
                     /translation="MTRISRTGFATASAVAALSLVFTTGCTTKKYVASQTQPVIQKTN
                     ELDDATAANNRAIHDVDSRAQAGIQQAQSSANQAQQSAQNASQAANTAQSSAQDAVNR
                     ADSLAGVIANLDNYKQVGDVSVTFGFDKSNLNKAAKEQLDQFGSQLANAKNYILEVTG
                     GTDSAGSKEYNYALSDRRAMAVVQYLASKYNVPAHKFYLVGLGKDVEVESNKTAEGRK
                     KNRHVEVQLLSNAGDNGAPAQGAANPTGGAPSGR"
     gene            complement(3314..3928)
                     /gene="pyrE"
                     /locus_tag="VHE33_02410"
     CDS             complement(3314..3928)
                     /gene="pyrE"
                     /locus_tag="VHE33_02410"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HVT96329.1"
                     /translation="MTSASENPRIALLDLLARTSFRLGNFTLSSGAKSDYYIDCRTTT
                     LHAEGGRLTGLAILDLLREQNLHPVAVGGLTMGADPVVSAVSIASAWRAQQEADAPLI
                     HGFLVRKAEKAHGTGRRIEGFLEKDAPVLIVDDVCTTGASTITAIEAAREAGMRVIAA
                     VCLVEREEAGGRAALEAACQGAPFLRLYTANDVRGSHVRQLQAR"
     gene            complement(4015..5334)
                     /locus_tag="VHE33_02415"
     CDS             complement(4015..5334)
                     /locus_tag="VHE33_02415"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HVT96330.1"
                     /translation="MKFRGLIVAAAVLLVLAGVLYWSEHHKPKESASATTPAILKIDP
                     ATVTSVTVKQKGAQAVTLARAGASQWKITSPGDYPADSSAVSAMLSSLAPLNSDSIVE
                     EKAENLSEYGLSDPSLEVDVTGKDNKTDHLLFGDDAPTGGGVYVQLAGSPRVFTAGSF
                     AKSSLNKSLGDLRDKRLLPVEASSVSSIDLDRKGQKISFARIQKGWQIEKPESYRTDN
                     YQVDDLLSQLTGAKWDPSVSAEDATRDFSHATPVATVKLTGSSGTDTLEVRKEKDAYY
                     AKSSAVPGAWKIDSSNASSLDSDLERGLDDFRNKQLFDFGYADPDKIEYHSGNTSIVL
                     ARSGKDWISDGKKMDSGSAEGLITAVRDLAASKFVTSGFTNAGIDLTVTSDGGKRVEK
                     VQMQKTSDGAIARRDDGPSLYSLDSAALTGLTDAIAGLKPATPEKKQ"
     gene            complement(5369..6745)
                     /locus_tag="VHE33_02420"
     CDS             complement(5369..6745)
                     /locus_tag="VHE33_02420"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HVT96331.1"
                     /translation="MASQWLKARQTKYAAYATTYTLVVLAVVVVANYLANRYSKQYDA
                     TANKRYSLSQETKKIVDGLKQDATITYFNVSTHFGEGKDILDEYKAISPKVHVKYVDP
                     VKEPMLARQAGIRNEGTATVQVGDHTEIASDMTEEGLTGALIRDIKGNTRTVCFVTGS
                     GEHQTDDSSREGESRFKDLLDRDSYQTKSISLVATASVPADCTAIVIDGPTRNYEQPE
                     VDAIKTYVQGGGRALFMLDPPLNMGPSSIGDNASLLSLLESWGVTADKDLVLDLNPIG
                     QLYGFGGQVALVNSYGTQPIVADMKTTFTGFPLARSLTFRNTDKSSVEKLFDTSDSSF
                     ATTNLSSPKINVKDPNNKRGPLTLGVAGTYNTGKSGSEGRFVVIGSSAWASNSFISFN
                     GNGDLATNSINWLTSDEDLISIRPKKPEDRRITMTSAQLNMVRLISQFVLPLIVIAGG
                     ILVWWKRR"
     gene            complement(6752..>7330)
                     /locus_tag="VHE33_02425"
     CDS             complement(6752..>7330)
                     /locus_tag="VHE33_02425"
                     /inference="COORDINATES: protein motif:HMM:NF024741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT96332.1"
                     /translation="QLIRPLLGTSSFLGLFMIPLISMRLFAEEKRNGTIELLATSPVR
                     DVEIIIGKWFSATLLYCWIIVFACINFVFLFRYGHPDWKPMLVTLLGVFLQGAALLAI
                     GTFISTLTKNQIIAAAMTFGACLLLWVFSWVSSYDSSTWAQVFSYLSVITHFDTFDRG
                     VLSTKDAIYYLSVIFVGLFFTARSMESLRWRS"
BASE COUNT         1466 a         2315 c         2086 g         1463 t
ORIGIN      
        1 ttcgaagctg gcacaacatg gcccaccacc gcgcggtcca gatccacgta ggccagctca
       61 agcgagccgt gactgcacag gccatcgaga aggaaggtcg atcgcagctc ctcggtggtc
      121 atgcgaggat aacgaaccgg atcggccatc aaaactgtct tcatcatcac tccatttgcg
      181 ggcgaagtct gtcacggccc gcacctcaca tcgggcagac tcttttgtct atcagaaggg
      241 cctggggact gtctgcgttt gcgatcggcc caatcccgca atacgggata ctccctccct
      301 tatcgtgaat cattcaggac tggcagagag gtcgttggtg tttccgcagc gtggaaatac
      361 gcgtgaatct cctccgcggt cttcggtgga accacagctg ccagcgattc cagattcgca
      421 accgcgatgc tccgcaggct gccaaagtgt tccagcaggc gctgccgtgt gcgtgcaccg
      481 actcccggga tggtcagaag ttctgaatcg cggtcgcgca tctcccgccg cttgcgatgg
      541 taggcaatgg cgaagcggtg cgactcatcg cgaatacgct gcaccagatg cagcaccgga
      601 gagcgccggt cgagcacgac cggctcgtct tcctgcccgt acagatagat gatctcctcc
      661 cgcttggcaa tcgacgccag cggctgcgtc gtaatcccca gagactccag cgcctccgcg
      721 gcggcgtgca gttggcccag tccgccatcg atcagaatca gagacggcat cttctgcttt
      781 tcttcgacga tgcgcttgta gcggcgtgtc acgacctcgc gcatcgaggc gaaatcgtcg
      841 acaccctcca cggttttgat cctgaacttg cgataggcgc tcttcttcat ctcgccgtct
      901 tcccatacca ccatcgacgc caccgtctcc gccccctgga tatgggagat gtcgaagcac
      961 tcgatggtgc gcggcaaatc ttcgagcgtc agcgcgtcct gcagcgcctc ctggattgcc
     1021 ttgcggcttg gcgcaagaac gcggaaacgc tggtcgaagc tctgttttgc attctgcccg
     1081 gccagatcga cgagcgaccg cttgtctccg cgctggggaa ccgcgatctc aacccggtgc
     1141 ttcgtgtgct cggccagcag actggccagc gacgagcgat ccgcgaaatc gaccggcaaa
     1201 tagatgctct tcggcacgta ctgctggtcg atgtagagct gcttcagcag ggcagagaag
     1261 aacgcccccg gctcaaacgt cgcctccgcc tgctcaacgg caaccgggcc gctctccgac
     1321 tccggcagtt cgccgacaaa atcaggcaga tcctcccaga aaaactcgcg ccggtccacg
     1381 atttttcccc cgcgcatgtg gaacaggttc accgcaatca tgccgctctc gctgtgaaag
     1441 ccgaaaacgt ccgcgtcgtc atcctcgacc gaagcgatgc gctgcctctc cgagagctgg
     1501 tcgatcgtca ccagcaggtc gcggtatttc gctgccagtt cgtactgctc agcagcggcc
     1561 gcgctggcca tgcgtgcgcg cagagatttg ttcagctcag ccgtgcggcc ttccagaaac
     1621 atctgcgcat cacggaccgc ttccgcgtaa gcctcgggcg tcgtcaggtc ctggacgcac
     1681 ggccccaggc agcgcccgat gtagtactgc aggcagggcc gcgagtggta gcgcgacaaa
     1741 tcaatcttgc agctgggcag gaggaagctg cggtggatca gatcgaccac gcgataggcc
     1801 agattgccgg ggaagtaggg tccatagtaa gcgctgccgt cccggcgcag ccgccgcgtg
     1861 acaaaaacct tcggccagcg atcgccgaga gtgagcttca cataggggta ggtcttgtcg
     1921 tcccgcagca ggatgttgaa gcgcggcttt cgctgcttga tcagattgtt ttcaagcgcc
     1981 agagcctcgt gctcgttgtc gaccaggatg tagtcgacgt cgaccgcgtc ccgcatcaga
     2041 gagccggttt tcgcgttcgc ctgggcggcg tccaacaggt acgagcgcac ccgggaccgc
     2101 aaatttcggg ccttgccgac gtagatcacc tcgccgtcgg cattcttata gaggtaaacg
     2161 ccgggccggg tgggtaaggt acgaatcttt tcgtgtaaat ccatgatggc cctggagcta
     2221 gtgtaacggc tggcccgcaa ggtggttttg ggggcagatc ggcctcaatt ccgcgtaaaa
     2281 aatacgcaag tggccaattt tccaggccgg aaaagagggg cgctgtttct ggttaaagca
     2341 tgatggaaca tagacttagg ctcatttatg agaattgggc ctgcgcgtgc accttcccga
     2401 ggcgaaaaaa ggaagcatcc gctcgcaaag cggagctttc tcagtagttg aaagaagcag
     2461 gacaacaata gaaatggagt gtcacccatg acgcggattt ccagaacagg tttcgcgacc
     2521 gcctcagctg ttgcggcgct gtctttggtt ttcacaaccg gatgcaccac caaaaaatat
     2581 gtggccagcc agacccagcc ggtaattcag aagaccaacg agcttgacga tgccacggcc
     2641 gccaacaatc gggcgatcca cgatgtggat tcgcgggccc aggccggcat ccagcaggca
     2701 cagagttcag ccaaccaggc gcagcagagc gcacagaacg catcccaggc cgccaacacc
     2761 gcccagtctt ccgctcagga tgccgtcaat cgcgccgact ccctggccgg cgtcattgcc
     2821 aatctcgaca attacaagca ggtcggcgat gtctccgtca cgttcggatt cgacaagtcc
     2881 aacctgaaca aggcggcaaa agagcagctg gatcagttcg gcagccagct ggccaacgcg
     2941 aagaactaca tccttgaagt cacgggcggg accgactcgg ctggttcgaa ggaatacaac
     3001 tacgccctga gtgatcgccg cgccatggcc gtcgtgcagt atctggcgtc gaagtacaac
     3061 gtgcccgcgc acaagtttta cctcgtcggt ctcggcaagg atgtcgaggt cgagtccaac
     3121 aaaaccgccg agggccgcaa gaaaaaccgt cacgttgaag ttcagttgct gagcaacgcg
     3181 ggcgacaacg gtgcacccgc ccagggcgca gccaatccaa ccggcggagc gccttccggc
     3241 cggtaaccct tagcgaagag acaaatcttt gggcgcgcgc ttctaagctg cgcgcccgtt
     3301 ttctttttgc actctacctg gcctgtagct gccggacgtg cgaaccccgc acgtcattcg
     3361 ccgtatacag ccgcaggaat ggcgcaccct gacaggccgc ttccagagca gcccgccccc
     3421 ccgcctcttc ccgctccacc aaacagaccg ccgcgatcac gcgcattccc gcctccctgg
     3481 cggcttcaat cgctgtgatc gtcgaggcgc ccgtggtgca cacatcatcc acaatcagca
     3541 ccggcgcgtc cttctcgagg aatccctcga tccgccggcc tgttccatgc gccttctccg
     3601 ctttgcgcac gaggaagccg tggatcagcg gtgcatctgc ctcttgctgt gcccgccacg
     3661 cgctcgcaat ggatacggca gacacgaccg gatctgcgcc catcgtcagt ccacccaccg
     3721 cgaccggatg aaggttttgc tctcgcaaca gatcgaggat ggccaggccg gttagccggc
     3781 ccccctcagc gtgcagcgtc gtcgtgcggc agtcaatgta gtagtcggac ttcgcgccgg
     3841 atgacagcgt gaagttgcca agccggaacg aggtgcgcgc cagcaggtcg agcagggcaa
     3901 tgcgaggatt ctcagaagca gaggtcatct cgcctagtgt aacggtccgc cacagcaggt
     3961 ttctcacgtt gtgctgaccg ccgatgccga acgccgagcg cggatcgccg aacgctactg
     4021 cttcttctcc ggtgtggccg gcttcagtcc ggcaatcgcg tccgtcagcc ctgtgagcgc
     4081 cgccgagtcc agtgagtaca gcgacggccc gtcatcgcgc ctggcaatcg ccccgtcgct
     4141 ggtcttctgc atctggacct tctccacccg cttgccccca tccgacgtca ccgtcagatc
     4201 gatgcccgca ttcgtaaatc ccgatgtcac aaacttcgaa gcagccagat cgcgcaccgc
     4261 agtgatcagc ccctcggcac tcccgctgtc catcttcttt ccatccgaga tccagtcctt
     4321 gccactcctg gccagcacga tggatgtgtt tccagagtgg tattcgatct tgtccggatc
     4381 ggcatagccg aaatcaaaca gctgcttgtt gcgaaaatca tccaggcctc gttcaaggtc
     4441 cgagtccagt gagctggcgt tgctcgagtc gatcttccac gcgccaggaa cagcgctcga
     4501 tttcgcgtag taggcgtctt tctctttgcg aacttcgaga gtatccgtgc cggagctgcc
     4561 tgtcagcttc accgtcgcaa ccggcgtggc atgggagaag tcgcgcgtcg catcttcagc
     4621 cgagaccgac ggatcccact tggcaccggt gagctgcgaa agcaaatcgt ccacctggta
     4681 gttgtccgtt cggtacgatt ccggtttctc gatctgccag cccttctgaa tgcgcgcgaa
     4741 gctaatcttc tgacccttgc gatccagatc gatgctgctg accgaactcg cctccacagg
     4801 cagcagccgc ttgtcgcgca ggtcgccgag gctcttgttc aggctgctct ttgcgaacga
     4861 acccgccgta aaaacgcgcg gacttccggc cagctggaca tacacgcctc ctccagtcgg
     4921 ggcgtcatct ccaaaaagca gatggtccgt cttgttgtct tttcctgtga catcgacctc
     4981 gagagatgga tcgctgaggc cgtattcgct gaggttctca gctttctcct caacaatgct
     5041 gtcggaattc agcggcgcaa ggctgctcag catcgccgat accgcgctgc tgtccgcggg
     5101 atagtcccca ggcgacgtga tcttccactg gctggcgccg gcccgcgcca gcgtaaccgc
     5161 ctgcgccccc ttttgcttca ccgtcacact tgtgaccgtc gccggatcaa tcttcaggat
     5221 cgccggagta gtggcggatg cgctctcctt cggcttgtgg tgctccgacc agtagagaac
     5281 acccgccagg acgagaagaa ccgctgctgc aacaatcaga ccgcgaaatt tcatgaccaa
     5341 tcttcagccg atcgaatccg gagttcggct atctccgctt ccaccacacc aggatgccgc
     5401 cggcaatcac aatcaggggc agcacaaact ggctgatcag ccgcaccata ttgagctgcg
     5461 cgctggtcat cgtgatgcgc cggtcttccg gctttttcgg acgaatggaa atcaaatcct
     5521 cgtccgatgt cagccagttg atggagttcg tcgccaggtc gccgttgccg ttgaagctga
     5581 taaagctgtt cgacgcccag gccgagctgc caatgaccac gaaccgtccc tcggagccgg
     5641 atttgcccgt gttgtaggtc ccggccacgc ccagcgtcag cggcccgcgc ttgttgttcg
     5701 gatccttgac gttgatcttc ggcgagctca ggttcgtcgt ggcaaagctg gagtccgacg
     5761 tgtcaaaaag cttttcgacg ctgctcttat ccgtattcct gaaagtcagt gaacgcgcca
     5821 gcgggaaacc ggtaaaggtc gtcttcatgt cggccacgat cggctgcgtc ccgtaactgt
     5881 tcaccagcgc cacctgcccg ccgaagccgt agagctgtcc gatcggattc aggtccagca
     5941 ccaggtcctt gtccgcggtc acaccccagc tctccagcaa cgagagcagg gaagcgttat
     6001 ccccaattga cgacggaccc atgttcagcg gcggatcgag catgaagagt gcgcggcctc
     6061 caccctgaac gtacgttttg atcgcgtcga cctcgggctg ttcatagttc cgtgtcggac
     6121 cgtcaatcac aatggcagtg caatcggccg gaaccgacgc cgtcgcgacc aggctgatcg
     6181 acttcgtctg gtagctgtcg cggtcgagca ggtccttgaa ccgcgattcg ccttcgcggc
     6241 tggaatcgtc ggtttggtgt tcgccgctcc cggtgacaaa acacacggtg cgtgtgttgc
     6301 ctttgatgtc ccggatcagc gcgcccgtca ggccttcctc cgtcatgtcg ctggcaatct
     6361 cggtgtgatc gcccacctgc accgtggctg ttccttcgtt gcggatgccc gcctggcgcg
     6421 ccagcatcgg ttccttgacc ggatcgacat acttcacgtg aactttgggc gagattgctt
     6481 tgtattcgtc gaggatgtcc ttgccttcgc cgaagtgcgt gctcacgttg aaataggtga
     6541 tggtcgcatc ctgcttcagc ccatcgacga ttttcttcgt ctcctgggac aggctgtaac
     6601 gcttgttcgc agtcgcgtca tactgcttcg agtaacggtt ggcgagataa ttggcgacca
     6661 cgacgacggc cagcacgacc agcgtataag tggtcgcgta ggccgcgtat ttcgtctgtc
     6721 tcgccttcag ccactgactt gccattcctg cttacgacct ccagcgaagg gattccatcg
     6781 atcgcgcggt aaagaagagt ccaacgaaga tgacgctcag gtagtaaatc gcgtccttcg
     6841 tcgacagcac accgcggtcg aaggtatcga agtgagtgat gacggagagg taggagaaga
     6901 cctgcgccca ggtcgacgag tcatagctgc tgacccagga aaagacccac agcaagagac
     6961 aggcgccaaa ggtcatcgca gccgcgatga tctgattttt cgtcagcgtc gagataaacg
     7021 taccgatggc cagcagcgcc gcaccctgca ggaaaacgcc cagcagcgtc accagcatcg
     7081 gtttccagtc gggatgccca tagcggaaca ggaaaacaaa attgatgcac gcgaaaacga
     7141 tgatccagca gtagagcagt gtcgccgaaa accacttgcc gatgatgatc tccacatcgc
     7201 gcaccggcga agtggccagc agctcaatcg tcccgttgcg cttctcctcg gcgaagaggc
     7261 gcatgctgat gagcggaatc atgaacagcc cgaggaagct cgaggtccca agcagcggcc
     7321 ggatgagttg
//
LOCUS       DASZYN010000092         8304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_516062, whole genome shotgun sequence.
ACCESSION   DASZYN010000092 DASZYN010000000
VERSION     DASZYN010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8304
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516062"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /locus_tag="VHE33_02430"
     CDS             <1..1295
                     /locus_tag="VHE33_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96333.1"
                     /translation="IQPMDTPLVFSGFGSGALQLWKEHAPAGLAPVEGVGGSASSEKQ
                     PDPLMPGSAVSAVLLRGDLNIAATCTVTYIDPKRMLACGHPITQFGPVSMPMTKADVV
                     ATLPSPLEAFKIINTTETVGSITEDRASAILGEFGKPARMIPVTLRVTRGGTGRAEGK
                     ESETLHFEVIDQPQITPMAVMVAVYQALMLENTYSAQTTYHVQGSVKLSGYPLVKLNS
                     LVAPTDALPANLQTALALGERFTRLYDNAARRTPVESVDLEVAAIPRRLTAEIQSVHT
                     SRVEVHPGDTVTVEATILPWHGEPHNVRVPVKLPANLPEGPVRLLVSDAGTLDRLMQP
                     PQFNAQPLDVAATIAQLNSVHTSDQLYITLLAPQAQAAVDGRTLTAIPMTVANALAPL
                     RENKGMALNGESAVPMASLPMDAVVTGQQVVTLQVEDQ"
     gene            1545..1985
                     /locus_tag="VHE33_02435"
     CDS             1545..1985
                     /locus_tag="VHE33_02435"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HVT96334.1"
                     /translation="MAYVERIRDMIGQPLLEEIVRQRVSAGWHLVSVEWRRELPESEM
                     PTDGAFPEDIPYGLRISEDCKGLELDPNEQQALLQMMEMLVQDFPFSNIASHLNEMGF
                     RTRDGKRWSPVAIFNMIPRLIDVGPRLFSLEEWEKRREKFARMI"
     gene            complement(2044..2877)
                     /locus_tag="VHE33_02440"
     CDS             complement(2044..2877)
                     /locus_tag="VHE33_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HVT96335.1"
                     /translation="MVFASCVRRPRWTATRTATDRWDAKSCAIERSDSRCARQRFIAH
                     ASHRVTHRIPIFMKAALAQTSAADFLPQSPAARRNLDSLRKAAAHCEGCPLFQRATQT
                     VFGEGKARARMILIGEQPGDREDIEGRPFVGPAGLLLDRCLEEAGIDRDDVYVTNAVK
                     HFKWEPRGKRRIHKKPSLREIAACRPWFDAEVSAVEPELIVCLGATAAQALLGSTFRV
                     TRNRGKVLTPQHLPPVLATVHPSSILRAPDEEARHEARTLFVADLREAAAFVHLKRRA
                     A"
     gene            2918..3427
                     /locus_tag="VHE33_02445"
     CDS             2918..3427
                     /locus_tag="VHE33_02445"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HVT96336.1"
                     /translation="MKVFSANLDNLRKLYINQLQMLLSTEQQITETLPKMIEKATDTQ
                     LKQAFQSHLQETEVQAQRLEQILNQATGDAKAVKCKVMAALATEGEDAVKDAADDAVR
                     DAALISAGQRVEHYEIAVYGAVRRWAQLLGETSHVELLDKTIKEEGHADHLLTEIADR
                     VNPYAQKAA"
     gene            3543..4055
                     /locus_tag="VHE33_02450"
     CDS             3543..4055
                     /locus_tag="VHE33_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96337.1"
                     /translation="MKKLLVVLFLLTAGVSAHSQTGIYGEFSAADYHRPTTNWQYGPT
                     FGLYSQPLGVPFVRAGFDIRGSFLGKGNEKVDSILGGLRVQVHPHVVPLMPYGEALAG
                     VAHVNVGQGSANTTGTGVEYQLVGGLDVTLLPHLDWRAIEYSWSSVHAPHSVTFEPQA
                     ISTGVVLRLP"
     gene            complement(4068..5879)
                     /gene="typA"
                     /locus_tag="VHE33_02455"
     CDS             complement(4068..5879)
                     /gene="typA"
                     /locus_tag="VHE33_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HVT96338.1"
                     /translation="MSIRNIAIIAHVDHGKTTLVDQLLRQAGTFRANETLTERVMDSN
                     ELERERGITILAKNTAVFYHNEKINIVDTPGHADFGGEVERALKMVDGVMLLVDASEG
                     PLPQTRYVLSKALEAKLPPILVINKIDRPDARAQEVLNEVYDLFIDLDASEDQLDFPV
                     LYTNAKLGQASIDPTVPGTDLMPLFDAILATIPTGKGDPTGVLQIQVTNLDYSDYLGR
                     LAIARVFRGTLHTGDDVAICKRDGSMQKTRITKMFSFSGLKRVDIEATEIDDIVAIAG
                     VEGITIGETITSAENPEPLPPIIIDEPTIAMQFSINNSPLVGREGQWVTSRNLRDRLE
                     KELLTNVSIRVEETGSPDSFKVLGRGELQLSILIEMMRREGYELMVGRPEIVTKEVDG
                     KRMEPVEHLTIDIPEGFIGTVIERLGPRRGEMVKMHNHGYGRVRLEFRVPSRGLIGLR
                     SDLLTDTRGTIVMNSLFDGYMPHQGDIPQRPTGALVADRPGITTAYALEGLEDRGVLF
                     TPPGVEVYEGMIIGEHSRDNDLDVNVVREKKLTNMRASTADDAIRLAPPHLMNLEQAI
                     EFIADDEMVEVTPKSLRLRKKILQSNRRPRRNPTVTA"
     gene            6103..6915
                     /locus_tag="VHE33_02460"
     CDS             6103..6915
                     /locus_tag="VHE33_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96339.1"
                     /translation="MDGMIRRSLALGTGLALSMTMGAAARAQSQPSAQPTTQQPTPQQ
                     PAAEQNPAPAASDAQPQRTIQMVQAKAQLDKTIDTKKAKQGDPVTAKLQDKVQIPEAQ
                     ELPKNTVLEGHVDQVQPSQNKGDAMVTVTFDKAKLKDGKEIPIKATVLAVSEPYYAQQ
                     PTAGGGAPSSGPAMMPSAPSRGTPSGGGSMGSGGASTPAVAPQPMNVPDASQGPSRTT
                     QSHGGVPDVMLKSDIHEHTSATFSSKGKNVHVPDGTQMQFALAIIPAGVTIQ"
     gene            complement(6935..8236)
                     /locus_tag="VHE33_02465"
     CDS             complement(6935..8236)
                     /locus_tag="VHE33_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT96340.1"
                     /translation="MATDRTDRPRPERTARLRSFERTIASARQTIMLSEIAKLAIDSF
                     RSSKVRFLLTALGMVIGTASVILVVAISATGRQYILSEIQGIGTNLVELEYAGGGSGA
                     VTNLQSDFLTFEDEHLIDEEVPTIAASSVVLEMHQNISLGGGITREILVLGVEPQYRQ
                     VRNLVTLSGRFFDEQDESMHGKVAVVTEPFARALFGSPGEAIGRSFQITGIPFTIIGT
                     FKERVETFGETEIADQTILIPYSVGRYFSGTDNVKQMYFSIRDQSAVEDAARQIKAAI
                     QHSGRHQPTSVYRTQTMTALITLAGEVVNALTAMLLLVSGVTLAVGGVGIMNIMLATV
                     RSRTREIGIRKALGATRREIKLQFLMEAGFVSLFGGLIGTIIGLGIPFSVRFFSAYRV
                     PVSGWSAVVGLVASVSVGIIFGTLPANRASAMDPIDSLRYE"
BASE COUNT         1565 a         2175 c         3033 g         1531 t
ORIGIN      
        1 tgattcaacc gatggatacg ccgctggtgt tcagtggatt cggctcagga gctctgcagc
       61 tgtggaagga gcacgcgccg gccggactgg ctccggtgga aggcgtgggc gggtcggcca
      121 gcagcgagaa gcagccggat ccgctgatgc cgggatcggc ggtaagcgcg gtgctgctgc
      181 gtggagatct gaacatcgcg gcgacgtgca cggtgacgta catcgatccg aagcgaatgc
      241 tggcttgcgg gcatccgatc acgcagttcg ggccggtttc aatgccgatg acgaaggcgg
      301 atgtcgtggc gacgctgccg tcgccgctgg aggctttcaa gatcatcaac accactgaga
      361 cggtgggatc catcacggag gatcgggcat cggcaattct gggcgagttc gggaaaccgg
      421 cacgcatgat tccggtgacg ctgcgggtga cccggggggg gacggggcga gcggagggaa
      481 aggaatccga gacgctgcac tttgaggtga tcgaccagcc gcagatcacg cccatggcgg
      541 tgatggtggc ggtgtaccag gcgctgatgc tggagaacac ctactcggcg caaacgacct
      601 atcacgtgca gggctcagta aagctgagcg ggtatccgct ggtgaagctg aatagcctgg
      661 tggcgccgac cgatgctttg ccggcgaatc tccagaccgc gctggcgctt ggggaacggt
      721 tcacgcgctt gtacgacaat gcggcgcggc gcacaccggt ggagagcgtc gacctcgaag
      781 tggcagccat tccgcggcgg ttgacggcgg aaatccagag cgtgcatacg agccgggtgg
      841 aagttcatcc cggtgacacg gtaacggtcg aagcgacgat tctgccctgg cacggcgagc
      901 cgcataacgt gcgggtgccg gtaaagttgc cggcgaacct gccggagggg ccggtgcgcc
      961 tgctggtaag tgacgccggt acgctggacc ggttgatgca gcctccgcag ttcaacgcgc
     1021 agccgctgga cgttgcggcg acgatcgcac agctgaacag cgtgcacaca agcgatcagc
     1081 tgtacatcac gctactggca ccccaggcgc aggcggcggt ggatgggcgc acgctgacgg
     1141 cgattccgat gacggtagcc aatgctctgg cgccgctccg ggagaacaag ggaatggcgc
     1201 tgaacggcga atcggcggtg cccatggcgt cgttgccgat ggatgcggtg gtgaccggcc
     1261 agcaggtggt tacgctgcag gtcgaggatc agtagggcgg ccggcagcgc gtagccgtgg
     1321 ctggttgggc ggcaaatctc agaagtccat cttcagccga ctgcggccgt tcaggcccgg
     1381 acaagtttcc caagaatgga catgcaaggc gtggcgacgg cgaattgagc ctcgggttac
     1441 gcacgtgctg tcatggttga gcccgttcgt cacaggcggc ttgcggtggt ccgtggcgtg
     1501 cagtctaatg ggcgcgacgc agaggttaga aagggaggca tcgcatggcg tacgtagagc
     1561 gcatccgcga catgattggc cagccgctgt tggaagagat tgtgcggcag cgggtttcgg
     1621 ccgggtggca tctggtttcc gtggagtggc ggcgcgaact gcccgaatct gagatgccga
     1681 ccgacggggc gtttccggag gacattcctt atgggctgcg catctcggaa gactgcaagg
     1741 ggctcgagct cgatcccaac gaacagcagg cgctgctgca gatgatggag atgctggtac
     1801 aggattttcc gttctccaac attgcgagcc atctgaacga gatgggtttc cgcacgcggg
     1861 atggaaagcg ttggagtccg gtagccatct tcaatatgat tccgcgcttg attgatgttg
     1921 gaccgcggct gttctcgtta gaggaatggg agaagcgacg ggagaagttt gcgcgcatga
     1981 tctgagggcg ggaatattca ggaggtgaag ggcaggggcg acgagcgtcc ctgccctttg
     2041 cgtttatgcg gcacgacgtt tgaggtgaac gaaggccgcc gcttctcgca ggtcggcgac
     2101 gaacagggtg cgggcctcgt ggcgggcctc ctcgtccggc gcgcgcagga tcgatgaagg
     2161 gtgcaccgtg gcgagcaccg ggggcagatg ttgcggggtt aagactttgc cgcgattcct
     2221 ggtgacgcga aatgtgctgc cgagcagggc ctgcgcggcc gtggcgccga ggcagacgat
     2281 tagttcgggc tcgacagcgc tgacttcggc gtcgaaccat gggcggcagg cggcgatttc
     2341 gcggaggctg ggctttttgt ggatgcggcg tttgccgcgc ggctcccatt tgaagtgctt
     2401 gaccgcgttc gtgacgtaga catcgtcgcg gtcgatgccg gcttcctcaa ggcagcggtc
     2461 gaggagcagt ccggcgggac cgacgaaggg cctgccttcg atgtcttcgc ggtcaccggg
     2521 ctgttcgccg atgaggatca tgcgcgcgcg ggcctttcct tcaccgaaga cagtttgggt
     2581 cgcgcgctgg aagaggggac aaccttcgca gtgggccgcc gctttgcgga gcgagtcgag
     2641 attgcggcgc gcggccggac tttgggggag gaagtccgcg gcagaggttt gggcgagcgc
     2701 tgccttcata aagattggga tgcggtgtgt gacgcgatgg gacgcgtgcg cgatgaatcg
     2761 ttggcgggcg catcgggaat cggagcgctc gatcgcacag ctcttcgcat cccacctatc
     2821 agtggccgtt cgcgttgcgg tccaccgcgg acggcgcaca caggacgcaa aaaccacgaa
     2881 accgaccccg caagccaggg ccccaggaga gttacttatg aaggtgtttt ccgcaaatct
     2941 cgataacctg cgcaagctct acatcaacca gctgcagatg ctgctttcga ccgagcagca
     3001 gatcacggag acgctgccta agatgatcga gaaggcaacg gacacgcagc ttaaacaggc
     3061 gtttcagtca cacctgcagg agaccgaagt gcaggcacaa aggctggaac aaatcctgaa
     3121 tcaggcgacc ggcgatgcca aggcggtcaa gtgtaaagtc atggccgcgc tggcaaccga
     3181 aggcgaggac gcggtaaaag atgcagctga tgacgcagtg cgcgatgcgg cgttgatctc
     3241 ggccgggcag cgtgtggagc actacgagat tgccgtttac ggagcggttc gccgctgggc
     3301 acagttactg ggcgagacgt cgcatgtgga acttctggac aagacgatca aggaagaagg
     3361 gcacgcggat catctgttga cggaaattgc cgatcgggtg aatccgtacg cgcagaaggc
     3421 ggcttaagag gattgccggt ggatcgtcca aggctgcgat ccgccggctg ttcctccgtt
     3481 tgacttcgcc ggtgttgcag agctaagggt cagggtgcaa catcggagga gaggcaagat
     3541 tggtgaagaa acttcttgtt gtgctcttcc ttttgaccgc gggcgtctcg gcgcactcac
     3601 agaccggcat ctacggtgaa ttcagtgcgg cggactatca tcgtccgacg acgaactggc
     3661 aatacggtcc cacgtttgga ctgtactcgc agccccttgg agtgccgttc gtgcgggcgg
     3721 ggttcgacat ccgtgggtca ttcctgggca aggggaatga gaaagtggac agcatcctgg
     3781 gcggcctgcg tgtgcaggtg catccgcatg tggtgccgtt gatgccctat ggtgaggcgt
     3841 tggccggcgt ggcgcacgta aatgtcggcc aagggtctgc caatacgacg gggaccggcg
     3901 ttgagtatca acttgtgggt gggctggacg tgacgctgct gccccacctg gactggcggg
     3961 ccattgaata ctcctggagt tcggttcacg cgccccacag cgtgaccttt gaacctcagg
     4021 cgatcagtac gggcgtcgtc ctgcggttgc cgtgaatccg gcgtaggcta cgccgtgacc
     4081 gtgggattgc ggcgaggccg ccggttcgac tgcaggatct tcttgcgcag gcgcagggat
     4141 tttggcgtga cttcgaccat ctcgtcgtcg gcgatgaatt cgatggcctg ttcgagattc
     4201 atcaggtgcg ggggcgcgag gcggatggcg tcatcggccg tagaggcgcg catgttggtt
     4261 agcttcttct cgcggacgac gttgacgtcg agatcattgt cgcgggagtg ctcgccaatg
     4321 atcatgccct cgtagacctc gacgccaggt ggggtaaaga ggacgccgcg gtcctcaagg
     4381 ccttccagag cgtaagcggt cgtaatgccg gggcggtcag ccaccagggc accggtggga
     4441 cgctggggga tgtcgccctg gtgtggcata tagccgtcga agagcgagtt catgacaatg
     4501 gtgccgcggg tgtcggtgag cagatcgctg cgcaggccga tgaggccgcg ggagggcacg
     4561 cggaactcga ggcggacgcg gccatatccg tggttgtgca ttttgaccat ttcaccgcgg
     4621 cgggggccga ggcgttcgat gacagtgccg atgaagccct cggggatgtc gatggtgagg
     4681 tgttcgacgg gctccatccg cttcccgtcg acttctttgg tgacgatctc ggggcgtccg
     4741 accatcagtt catagccctc gcggcgcatc atctcgatca ggatggagag ctggagttcg
     4801 ccgcggccaa gaactttgaa gctgtcggga ctgccggttt cttcgacgcg aatggagacg
     4861 ttggtgagca gttccttttc gaggcggtcg cggaggttgc gcgaggtgac ccactggcct
     4921 tcgcgaccga ccagggggga gttgttgatg gagaactgca tggcgatggt gggctcatcg
     4981 atgatgatgg gcggcaacgg ctcaggattt tcggcactgg tgatggtttc gccgatggtg
     5041 atgccttcaa cgccggcgat ggcgacgatg tcgtcgatct cagtcgcttc aatgtcgacg
     5101 cgcttgaggc cgctgaaact gaacatcttg gtaatgcgcg ttttctgcat cgagccgtcg
     5161 cgtttgcaga tggccacgtc gtcgccggtg tggagggttc cccggaaaac gcgggcgatg
     5221 gcgaggcggc cgaggtagtc ggagtagtcg aggttggtca cctggatctg caaaacgccg
     5281 gtggggtcgc ctttgccggt ggggatggtc gcgaggatgg cgtcgaagag gggcatcaag
     5341 tcggtgccgg ggacggtggg gtcgatgctg gcctggccga gctttgcgtt ggtgtagagg
     5401 acggggaagt caagctggtc ctcgctggcg tcgagatcga tgaagaggtc gtagacctcg
     5461 ttgagaactt cctgggcgcg cgcgtcgggg cggtcgatct tgttgatgac gaggatgggc
     5521 gggagcttgg cctccagcgc tttggagagg acgtagcgcg tttgcggcag gggaccttca
     5581 gaggcgtcga cgaggagcat gacgccgtcg accatcttga gggcgcgttc gacctcgcca
     5641 ccgaagtcgg cgtggccggg ggtgtcgacg atgttgatct tctcgttgtg gtagaagacg
     5701 gcggtgtttt tggcaaggat ggtgatgccg cgctcgcgtt cgagctcgtt ggagtccatg
     5761 acgcgctcgg tgagggtctc gttggcgcgg aaggtcccgg cctggcggag gagctgatcg
     5821 acgagggtgg tcttgccgtg gtcgacgtgg gcgatgatgg cgatgttgcg aatgctcact
     5881 gggtttcttt tctgttggtt tgagactgga acggtagttg cacagggatg gataagtgcc
     5941 tgcgtctgca ggctgtatcc cttcatgcag gatgtctgaa tgcgggtgcg gatactgacg
     6001 gtctcagcgc cgcctgtcta agtttaccag tcccgggcaa cgccctttga ctgcaggtgc
     6061 gtcctcctaa gggagggaac ggggcgcgcc agagggggtt ctatggatgg aatgattcgc
     6121 cggagtcttg cgctgggaac ggggctggct ctgagcatga cgatgggcgc agcggcccgg
     6181 gcgcaaagcc agccgagcgc acagccaacg acgcaacagc caaccccgca gcagccggcc
     6241 gccgagcaga atccggcgcc agcagcgagc gacgcgcagc cgcagcgcac gattcaaatg
     6301 gtgcaggcaa aggcacagct ggataagacc atcgacacca agaaggccaa gcagggcgat
     6361 cctgtgaccg caaagctgca ggacaaggtg cagattcccg aggcgcagga gctgccgaag
     6421 aacacggtgc tggaagggca cgtcgatcag gtccagcctt cgcagaacaa gggcgacgcg
     6481 atggtgacgg tgacgttcga caaggcgaag ctgaaggacg ggaaagaaat acccatcaag
     6541 gctacggtgc tggcggtttc cgaaccgtac tatgcgcagc agccgacggc tggaggcggc
     6601 gcgcccagca gcgggccggc catgatgcca agcgcgccga gcaggggcac accctcggga
     6661 ggtggatcga tggggagcgg cggggcgtcc acgccggcag ttgctccgca accgatgaat
     6721 gtgcccgatg ccagccaggg cccatcgcgg acgacgcaga gccatggcgg cgttccggac
     6781 gtgatgctga agagcgacat tcacgaacac acgtcggcaa cgttcagctc gaaggggaag
     6841 aacgtgcatg tgccggacgg gacgcagatg cagtttgctc tggcgatcat tcccgctgga
     6901 gttactattc agtaactagg ggcattgaag cccgctactc gtaccgcagc gaatcgattg
     6961 gatccatcgc ggaggcacga ttagcgggca gagtgccgaa gatgatgccg acggagacgg
     7021 aggccacgag tccgacgacc gcggaccagc cggagacggg gacccggtag gccgagaaga
     7081 agcggacgga aaagggaatg ccgaggccga tgatggttcc gatgaggcct ccgaagagcg
     7141 agacgaaacc agcctccata aggaactgaa gcttgatctc acggcgggtg gcgccgaggg
     7201 ctttgcggat gccgatctcg cgggtccggg agcggaccgt ggcgagcatg atgttcatga
     7261 tgccgacgcc gccgacggcg agagtgacgc cggagacaag aaggagcatc gcggtgagcg
     7321 cattgacgac ttcacccgcc aaggtgatga gtgcggtcat ggtctgggtg cggtaaacgg
     7381 aagtgggctg atggcggccg ctgtgctgga tggcggcctt gatctgccgc gccgcgtcct
     7441 cgacggcgct ctggtcgcgg atggagaagt acatctgctt cacgttatcg gtaccgctga
     7501 agtagcggcc gaccgaatag ggaatgagga tggtctgatc ggcgatctcg gtttcgccga
     7561 aggtctcgac gcgctctttg aaggtgccga tgatggtgaa gggaatgccg gtgatctgaa
     7621 agctgcggcc gatggcctcg ccgggagatc caaacagggc gcgtgcgaag ggttcggtaa
     7681 ccactgcgac tttgccgtgc atggattcgt cctgctcgtc gaagaagcgg ccggagaggg
     7741 tgaccaggtt gcggacctgg cggtactgag gctcgacgcc gaggacgagg atctcccggg
     7801 tgatgccgcc gccgaggctg atgttctggt gcatctcgag gacgacggag gaagcggcaa
     7861 tggtgggcac ctcttcgtcg atgaggtgct cgtcttcgaa ggtgaggaag tcggactgga
     7921 ggttggtgac ggcgccggag ccaccgccgg catactcgag ctcgacgagg ttggtgccga
     7981 ttccctgaat ttcgctaagg atgtactggc gaccggtggc ggagatggcg acgacgagga
     8041 tgacggaagc ggtgccaatg accatcccga gggcagtgag caggaaacga accttggagc
     8101 tgcggaagct gtcgatcgcg agcttcgcga tctcgctgag cataatggtc tggcgagcgc
     8161 tggcgatggt gcgttcgaag gagcgcaggc gggcggtgcg ttcggggcgg ggacgatcgg
     8221 tgcggtcggt ggccatctac ttcttcctag aggatgccac agggacgggg ttgggcgcgt
     8281 cgggggcaat ttttgaaaaa aggg
//
LOCUS       DASZYN010000093         5166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_4528, whole genome shotgun sequence.
ACCESSION   DASZYN010000093 DASZYN010000000
VERSION     DASZYN010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4528"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VHE33_02470"
     CDS             complement(<1..582)
                     /locus_tag="VHE33_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96341.1"
                     /translation="MSLLLLSMLGGVSGCRRKHFPKYPSDFREYAWVTNGGGNSVTVL
                     DLVHMTTAATIAVGDNPVDVAVNPVRDEVYVVNRGSGSVSVIDATTNKVTATMGVHRE
                     PESIGVDATGERAYVANAGSNNVSVLDLKKRREIGAVGVGEAPGLVRVSPDGGTLVVT
                     NRAGNSATVVDAEKLKVRAVFGGCPGASDAVILP"
     gene            complement(986..2044)
                     /gene="aroF"
                     /locus_tag="VHE33_02475"
     CDS             complement(986..2044)
                     /gene="aroF"
                     /locus_tag="VHE33_02475"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HVT96342.1"
                     /translation="MLVVMKAQATPEEIQAVCEHIEGLGYRAHALPGAQRTAIGITGN
                     QGEVDRGGIEELAGVAEVIRVSKPYKLVSRDVKEEDTVIRFAGTDATIGGAKLAMIAG
                     PCSIESREQAFAIAEQVAAAGAQFFRGGAYKPRTSPYAFQGLGEEALKIMAEIRERFG
                     LRIVTEAIDHEALELVAEYADVIQIGARNMQNFSLLKAAGRKRKPVLLKRGLAATLEE
                     FLMAAEYIMSEGNYEVILCERGVRTFADHTRNTLDLSIVPAVQRESHLPILVDPSHGT
                     GKRNKVLPLARAAVAVGADGLIVEVHHQPDKALSDGMQSIVPEQFEKLMDECGQIATV
                     LHRSVPRGIHVTESAGAR"
     gene            2663..3016
                     /locus_tag="VHE33_02480"
     CDS             2663..3016
                     /locus_tag="VHE33_02480"
                     /inference="COORDINATES: protein motif:HMM:NF014332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HVT96343.1"
                     /translation="MRWFLRSKLHNATVTEANIDYIGSITIDVALMEAVGLEVGEKVL
                     VVSNTSGARLETYTIAGERNSGTVQINGAAAHLIRKGEQVIVMGFELTDTPLQPKVLL
                     TGADNRAPQWLSEAG"
     gene            complement(3039..4688)
                     /locus_tag="VHE33_02485"
     CDS             complement(3039..4688)
                     /locus_tag="VHE33_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HVT96344.1"
                     /translation="MSLRHKFRLLVTVAVCGLVVMGALWLSGERSRLLAGKEEQSRAV
                     VELAYATIEAQYARAQAGQLNVEQAQENAKQMVRAMRYGDKNYLWINDLRPVMIMHPF
                     KPAMEGKDLNSYVDPKGTRIFVEFAKIAREHGSGTLYYWWPKPGESDPVRKVSYVRVF
                     QPWGWVIGTGTYIDDVNGMWRKSALQSGGVVLLCLVALLVLSTTISRSMVRRLDRLAE
                     RIRDVAQGEGDLTQRIAIDSKDEIAAAARWFNQFMDSLQETMGRVAGNTARLTSATTE
                     ISAAAAREAEGSKQQSGEITQVATAMEEMAATVAEVSTNANRVAEDARHAVEVAGQGG
                     AIVQGALGRMQSIAESVGTTARRIEELGKRSDEIGRIVAVIEEIASQTNLLALNAAIE
                     AARAGEHGRGFAVVAGEVRNLAERTTQATREIAGTIEAVQQETATAVEQMEAGTRLVE
                     QGVAETAKAGSALDEIITSSQHVGDMIAQIATTATQQSTAVGEINTSVAHIATIAHRG
                     EEAARQSATTCENLTGLAEDLHQTVGRFRLANGPAAHASAA"
     gene            complement(4711..>5166)
                     /locus_tag="VHE33_02490"
     CDS             complement(4711..>5166)
                     /locus_tag="VHE33_02490"
                     /inference="COORDINATES: protein motif:HMM:NF025062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CZB domain-containing protein"
                     /protein_id="HVT96345.1"
                     /translation="AEASTNLSRLANELDGTIRGFRICDESSGRGCGEMNFDRAVEAH
                     AKWKQKLAAYIAKPDRSLNADETGRDDGCELGQWLHGKGRRFADRPEFAQLIRDHAEF
                     HAAAGEIIRGADAGRITGQEIALGGRSRYAQVSTAVVGSLMKIKAALAV"
BASE COUNT          884 a         2008 c         1285 g          989 t
ORIGIN      
        1 cggcagaatc accgcatcgg aagcgccggg gcacccaccg aacaccgccc gcaccttcag
       61 cttctccgca tccaccaccg tcgcgctgtt ccccgcgcgg ttcgtcacca ccagcgttcc
      121 gccatccgga gaaacccgca ctagtcccgg cgcctcaccc accccgaccg cgccaatctc
      181 acgccgcttc ttcaggtcca gaaccgatac gttgttcgat cccgcattcg ccacgtacgc
      241 ccgctcgccc gtcgcgtcca cgccgatcga ttccggctcc cgatgcaccc ccatcgttgc
      301 cgtcacctta ttcgtcgtcg cgtcaatcac gctcaccgac ccgctgccgc gattcacgac
      361 gtacacctca tcgcgcaccg ggttcacagc aacgtccacg ggattatcac ccaccgcaat
      421 cgtcgccgcc gtcgtcatat ggacgagatc cagcacggtg accgaattcc cgccgccgtt
      481 cgtcacccac gcgtattcac gaaaatcgga aggatacttc ggaaaatgct tccgccggca
      541 gccgctcacc ccgcccagca tcgacagcaa cagaaggctc acgacaaccg ctgtcaggcg
      601 caccctgacg cgcgtctttg tatcaggaca ccaacccccg ggtgccccat cctggtggcg
      661 cctgccgttg ggcgcaagca gggtgggatt gcgaagtcct ctcccagccg cttttgtaac
      721 agggcacgag tcaaaaggcg gcccatattc ctggtgctgg gataggggag gccgcaaacc
      781 caaaccagct gcttttgtaa cagggcacga cttcagtcgt gccgaacagg cgccaggaag
      841 aaccggggct tgagcccctg aggctctccc caaattcggc tcggacttgc cttcagattc
      901 cacggtccgg ttcagcatac acgccagccg cacgcacgaa gcttcgcgtt ctcccgcacc
      961 ttcattggcc gagaagggaa tccgcctacc gcgctcccgc gctctccgtc acatgaatcc
     1021 cccgcggcac gctgcgatgc agcaccgtcg caatctgtcc gcactcgtcc atcagcttct
     1081 caaactgctc cggcacgatc gactgcatcc cgtcactcag cgccttgtcc ggctggtgat
     1141 gcacttcaac gatcaacccg tccgcgccca ccgccaccgc ggccctcgcc agcggcagca
     1201 ctttgtttcg cttgcccgtt ccgtggcttg gatccaccag gatcggcaag tggctctccc
     1261 gctgcaccgc cggcacaatg ctcagatcga gcgtattccg cgtgtgatcc gcgaacgtcc
     1321 gcaccccgcg ctcgcacagg atcacctcgt agttcccctc gctcatgatg tattcagccg
     1381 ccatcagaaa ttcttccagc gtcgccgcca gcccgcgctt cagcagcacc ggcttgcgct
     1441 tccgccccgc cgccttcagc agcgaaaaat tctgcatgtt gcgcgcgcca atctggatca
     1501 catccgcata ctcggccacc agctccagcg cctcgtgatc gatcgcctcc gtcacaatcc
     1561 gcaagccaaa ccgctcgcga atctccgcca tgatcttcag cgcttcctcg cccaaaccct
     1621 ggaacgcata cggcgaagtc cgcggcttgt aggcgccgcc ccggaagaac tgcgccccgg
     1681 ccgccgccac ctgctcggca atcgcaaagg cctgttctct ggattcgatc gaacacggcc
     1741 cggcaatcat cgccagcttc gccccgccga tcgtcgcatc ggtccccgcg aaccgaatca
     1801 ccgtgtcctc ttccttcacg tcgcggctca ccagcttgta cggcttcgag acgcggatca
     1861 cctcggccac cccggccagt tcctcaatgc cgccccgatc cacttctccc tggttgccgg
     1921 tgattccaat cgccgtccgc tgtgctccgg gcagcgcatg cgcccgatat cccagccctt
     1981 caatgtgctc gcaaaccgcc tgaatctctt ccggcgtcgc ctgcgccttc atcacgacaa
     2041 gcatcgtggt ttcctcactt gagctgcctc aagtgtagtg gatgggatac ggaaaggaga
     2101 aaatcagcag gaaaagggcg gttacgactg ggctattcga cggtgactgc cggagcccta
     2161 ttcgcctcga aacaggtccc gtccgctccg tgccgcaatc acggccgggg tcagaaccat
     2221 agcaacaaac acaatcacga tcagagcgtc acgcataggt ttggcgccgc ccggcctgtc
     2281 cgccgggttc tacgccagtc tccactttgc aggctcccaa acgaatagca cttcggcgtc
     2341 tccgcctgcg gccaaagcgt cagccgtgcc caaccagcca accccctcat tgctccctgt
     2401 taaaaaattg aatcgatccg caactttgcc ttcacccagg ggtccgtacc cagcgtgagg
     2461 ggtcccggcg ggcctttcac acagcaactc tcctcggaca acgaggctgc cacatctgtt
     2521 gtcagcggca gcctcgttga tttttcgtga aaccgtcggc agcctctcat ccacgcactt
     2581 tcatcccctg cgccggacgc ataccataga ggaacagagc cgctgcatcc gtacctcgga
     2641 gcggcaggcg ggaagggaag ttgtgcgctg gtttctccgc tccaagcttc acaatgccac
     2701 ggtcaccgaa gccaacatcg actacatcgg cagcatcacc atcgatgtcg ctctcatgga
     2761 agcggtcggc ctcgaagtcg gcgaaaaagt cctcgtcgtc agcaacacct ccggcgctcg
     2821 cctcgaaacc tataccatcg ccggcgagcg aaactccggc accgtccaga tcaacggcgc
     2881 cgccgctcac ctgatccgca aaggcgagca ggtcatcgtc atgggcttcg agctcacgga
     2941 cacccccctt caacccaaag tccttctcac cggtgccgac aaccgcgcgc cccaatggct
     3001 ctccgaagcc ggctgacggc cctcccaccc ccgtttcctc aggcagccga ggcgtgcgcc
     3061 gccgggccgt tcgccagccg gaaccgccct accgtttggt gcagatcctc ggcaagcccg
     3121 gtcagattct cgcaggtggt tgccgactgc cgcgccgcct cttctccccg atgggcgatc
     3181 gtggcaatgt gcgccacgct ggtattgatc tcccccaccg ccgtcgactg ctgcgttgcg
     3241 gtggtcgcaa tctgcgcgat catgtcccca acgtgctgag acgaggtgat gatctcgtcc
     3301 agcgccgacc cggccttcgc ggtctctgcg acaccctgtt ccaccagccg cgttcccgcc
     3361 tccatttgct ccacggcggt cgcggtttcc tgctgcaccg cttcgatcgt ccctgcaatt
     3421 tcccgagtag cctgcgtcgt ccgctccgcc aggttgcgca cctcccccgc caccaccgca
     3481 aatccgcgcc cgtgttcgcc ggcccgcgcc gcctcaatcg ctgcgttcag tgccagcaga
     3541 ttcgtctggc tcgcgatctc ctcaatgacg gcaacaatcc tgccgatctc atccgaacgc
     3601 tttcccagct cttcgatccg ccgcgccgtc gttcccaccg actcggcgat cgactgcatc
     3661 cggcccagtg ctccctgcac gatcgctccc ccctggccgg ccacttccac cgcgtgacga
     3721 gcatcctccg caacacggtt cgcgttggtt gacacttcgg ccaccgtggc cgccatctcc
     3781 tccatcgccg tcgccacctg tgtgatctcg ccgctttgtt gctttgatcc ctccgcttca
     3841 cgggccgctg ctgcggatat ctctgtcgtt gccgacgtca gccgcgccgt atttcccgcc
     3901 acgcgcccca tggtctcctg caggctgtcc atgaactggt tgaaccatcg tgccgccgcc
     3961 gcaatctcat ccttgctgtc gatcgcaatc cgctgcgtca ggtctccttc gccctgcgct
     4021 acatcccgga ttcgctccgc cagccggtcc agccgccgta ccatcgaccg cgagatggtc
     4081 gtcgacagca ccagcagcgc caccagacac agcagcacga ccccgcccga ctgcagcgcg
     4141 ctcttccgcc acatcccgtt cacgtcgtcg atgtacgtgc cggtcccgat cacccagccc
     4201 cacggctgga agacgcgcac atagctgacc tttctcacag ggtccgactc tccgggtttc
     4261 ggccaccagt agtagagggt tccgctcccg tgctcccgcg cgatctttgc gaactccacg
     4321 aagatgcgcg ttcctttcgg atccacatag ctgttcagat ccttcccctc catcgccggc
     4381 ttgaacgggt gcatgatcat caccggccga aggtcgttaa tccacaggta gtttttatcc
     4441 ccgtagcgca tggcgcgcac catctgcttt gcgttctcct gggcctgttc cacgttcagc
     4501 tgaccggcct gcgcccgcgc gtactgcgcc tcgatcgtcg cgtacgccag ctccaccacc
     4561 gctcgcgact gctcctcttt gcccgccagc aaacgggagc gctcgccgct cagccacagc
     4621 gcgcccatca ccaccaaacc acacaccgcc accgtcacca gcagtctgaa cttgtgccga
     4681 agactcatcc cgttccttcc cttgccgcct ctacaccgcc agtgcggctt tgatcttcat
     4741 cagcgatcca accacggccg tcgacacctg tgcataccgg ctccgtccgc ccagggcaat
     4801 ttcctgcccc gtaatcctgc cggcgtccgc gccgcgaatg atctcccccg ccgccgcgtg
     4861 aaactccgcg tgatcccgga tcagctgcgc aaactccggc cgatccgcga atcgccggcc
     4921 cttcccgtgc agccactgtc ccagctcgca tccatcgtcc cggcctgtct catccgcgtt
     4981 caggctgcgg tccggcttcg caatgtaagc cgccagcttc tgcttccact ttgcatgcgc
     5041 ctcgaccgcg cggtcgaagt tcatctcccc gcatccccgc cccgaactct catcgcagat
     5101 cctgaacccg cggatcgtcc cgtccagctc attcgccagt ctcgacaggt tcgtgctcgc
     5161 ctccgc
//
LOCUS       DASZYN010000094         7460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_839890, whole genome shotgun sequence.
ACCESSION   DASZYN010000094 DASZYN010000000
VERSION     DASZYN010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839890"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="VHE33_02495"
     CDS             complement(<1..1232)
                     /locus_tag="VHE33_02495"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HVT96346.1"
                     /translation="MRNCFAGLTVAAVLAALPALGQSSYTPPDSATKELARGIFKQIV
                     EINSTDSVGSVTAVSKAVQQRFIDAGFPAEDIYLGGPNDRKENLVVRYRGTGAHPPIL
                     FIGHEDVVEARREDWTTDPFQFIEKDGYFYGRGTQDMKENDAILVTTFLRLHKEGFKP
                     DRDLILALTADEEGGKSNGVDWLLKNKPELVKAEFAFNPDGGGVELQNGKAISADVDA
                     TEKLYADYQLITTNPGGHSSLPVPDNAIYHIADALVRVQHYAFPFELNAITRPYFEKL
                     ATMEPAETAADMRAMLATPPNKDAIARLSKSPEWNSTMHTTCVATRLLAGHANNALPQ
                     MAQANINCRILPGHSREEVRQDIIRIINDPKITVNYVNDGGTVFPAAPAAKGLPPVAL
                     KPEVMKPLEKLVAAYWPGT"
     gene            1408..3429
                     /locus_tag="VHE33_02500"
     CDS             1408..3429
                     /locus_tag="VHE33_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HVT96347.1"
                     /translation="MAAMITGTAAAQPASSSQPGFVNTLMPQPAHLTAGEGWLPLAPG
                     FSVGFDKTHDERLEAAVHRDLQRLEDSTGVMVQHATPQATATLTISVDGPGEEIQGID
                     EDESYSLEITPQHAQLHAATDVGAMHGMETLLQLVQADARGFWLPAVSIQDAPRFPWR
                     GLMIDVGRHFEPVSVIERTLDALAAVKMNVFHWHLTDDQGFRIESRIYPKLTGMGSDG
                     QYYTQDEARAVVAYARARGIRVVPEFDIPGHTTSWMVGYPDLASAPGPFSIERHFGVF
                     NPVMDPTRDSTYEFLDKFIGEMADIFPDAYMHIGGDENNGVEWKSNSKIQAFATAHNL
                     HGTAAIQAYFNQRLLPILTKHGKKMIGWDEVLTPDLPKDVMVQSWRGFDSLAAGAKQG
                     YSGILSAGYYLDHMDPASAHYRVDPVPANSDLTPEQAARILGGEACMWSEYVGPQTIE
                     SRVWPRTAAIAERLWSQRTVTSEDDMYRRLAVESLRLEQLGLTHISHEGTSLRALAGT
                     EDIDTLRPLTAVLEPVDFDTRSHWSEHNGVTTLSPFDSLVDALPPDPPSRHDFGDLMS
                     TYLQDPVGHPEIEAALEQTFRSWSADPHRMELLSSSPRLAAALPRAQQLAEMGTMGLE
                     ALSFLSSGLSAPPGWKAQKLAVLDEAQKPVALTRFTVLPAMRALVNEVH"
     gene            complement(3466..3915)
                     /locus_tag="VHE33_02505"
     CDS             complement(3466..3915)
                     /locus_tag="VHE33_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96348.1"
                     /translation="MPFTQNALDQFFSTYERDSLSDDPAKAAGHFAEHFLAAGPQGPV
                     TVPAAVFAQKLPARKQLFNDAGLTSTELTSRRDLRLGDRYVLVETEWKMNFRPESQPD
                     TNLAVSSSFLIDMGSSEPKIIAYIPHQDVFQLMKERKLLPPDEGPGS"
     gene            complement(4077..5189)
                     /gene="mnmA"
                     /locus_tag="VHE33_02510"
     CDS             complement(4077..5189)
                     /gene="mnmA"
                     /locus_tag="VHE33_02510"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HVT96349.1"
                     /translation="MSLNNTIAVAMSGGVDSSTVAAMLRADGYTLVGLTLQLWNQRRL
                     AGKEGMPEQVTGRCCSLDDVYDARRVAEALDIPYYVVNEQERFEDDVVRPFVREYLNG
                     RTPIPCSLCNNHLKFDQLLVRARQIGADRIATGHYARNEYDPTRNRWILSRPVDESKD
                     QTYFLFGLTQEQLSRTLFPLGSYRKPEVRALAAQHGLAVAVKPDSQEICFIPGGDYKK
                     FISAYLHEQGEEIPDTAGELVSTTGEVIGEHGGIHNFTIGQRKGLGVASPDPLYVIQI
                     EPASHRVTVGSDAEATTRTLRARDLNWISIPSLDGEMRVRAKIRHRHEPAWATIRMVG
                     QDEVEAVFDDLQRAVTPGQAAVYYDGDEVVGGGWIL"
     gene            complement(5283..6059)
                     /locus_tag="VHE33_02515"
     CDS             complement(5283..6059)
                     /locus_tag="VHE33_02515"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HVT96350.1"
                     /translation="MSIPALQLDGRTAVVVGGTSGIGRAISIGLAESGADVVASARRQ
                     EQVDETAAAIETTGRKCLRLTCDTQDRGSLQRLLDESVKTFGKVDILVNSAGKIKRAP
                     TLTFPEDEWRDIVDTNLTGTLRACQIFGAHMLERGYGRIINIASLNSYVALAEVAAYA
                     ASKAAVASLTRSLAVEWSKKGVLVNAIAPGVFRTALNSDLLDNTPRGKELLMRTPMAR
                     FGKTEELVGAAIYLASEAASFVTGQVLVVDGGFLASGVNQ"
     gene            complement(6056..7348)
                     /locus_tag="VHE33_02520"
     CDS             complement(6056..7348)
                     /locus_tag="VHE33_02520"
                     /inference="COORDINATES: protein motif:HMM:NF027582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HVT96351.1"
                     /translation="MATTELGKFSIGVGDRFAHQAKAQLQACIEAGRSGIEVIPVWNK
                     SNREHNIIGSEPSSTRAAADAAVKDLGWTKPYFCDADHINLTTVDRFLAPCDFFTIDV
                     ADYIGKPAEPASIEAFLKRRSDLKGAFRLEGVREPIQITPELLRETATKFLFAVQEAG
                     KVYRKIEGLKGKGNFVPEVSMDETDAAQTPAELLIILAAIADEGIPIQTIAPKFTGRF
                     NKGVDYVGNVDQFRREMILDVAAIAWATANLGLPKNLKLSVHSGSDKFSIYAAIHDAT
                     KESGVHLKTAGTTWLEEIIGLAEAGGAGLELAKEIYAQAYEHREELCGPYATVIDIDP
                     AKLPAPSVVAQWTSAQFTSALRHDPHSPAYNPSLRQLLHVGFKIAAKMGDRYIALLES
                     NEKVIAKNVTENLYDRHIAPVFLGKPSPAAALAHAEVQ"
BASE COUNT         1430 a         2314 c         2212 g         1504 t
ORIGIN      
        1 gtacccggcc aatacgcagc cacaagcttc tccagaggct tcatcacttc cggcttcagc
       61 gcgaccggcg gcaggccttt ggctgccgga gcagcgggga aaaccgttcc gccatcgttc
      121 acgtagttca cggtaatctt cgggtcgtta atgatgcgga tgatgtcctg gcgcacctct
      181 tcgcgcgagt ggccagggag aatgcggcaa ttgatgttgg cctgggccat ctgcggcagc
      241 gcgttgttcg catggccggc cagcagccga gtggcaacgc aggtggtatg catggttgag
      301 ttccactccg gcgatttcga gaggcgcgca atcgcatcct tgttcggggg cgtcgccagc
      361 atggctcgca tgtcggcagc ggtctctgcc ggttccatgg tggcaagctt ttcaaaatag
      421 ggacgggtga tcgcgttcag ctcaaacgga aacgcatagt gctggacgcg caccagcgca
      481 tcggcgatgt ggtagatggc gttgtccggc accggcagtg agctgtgccc gcccggattg
      541 gtcgtgatga gctggtagtc cgcatacagc ttttccgtcg catccacgtc cgcggagatc
      601 gccttgccgt tctgcaactc gacgccgcca ccatccggat tgaaggcaaa ctcggccttc
      661 accagctccg gcttgttctt gagcagccag tccaccccgt tcgatttgcc accttcctca
      721 tccgcggtca gcgccagaat cagatcgcga tccggcttga agccctcctt gtgcagccgc
      781 aagaacgtcg tcaccaggat tgcatcgttc tccttcatgt cctgcgtgcc gcgtccgtag
      841 aaataaccgt ccttctcgat aaactgaaat ggatccgtgg tccagtcttc acgccgtgct
      901 tcgacgacat cttcatgacc gatgaagaga atcgggggat gggcgcccgt cccgcgatac
      961 cgcaccacca ggttttcctt gcgatcgttc ggacctccga ggtaaatgtc ttcggccgga
     1021 aaccctgcat cgatgaagcg ctgctgcacg gctttcgata ctgccgtgac gcttcccacg
     1081 gaatccgtgc tgttgatctc gacgatctgc ttaaaaatgc ccctggcaag ttctttggtc
     1141 gcggaatcgg gcggagtgta cgaggactga cccaatgccg ggagtgcggc aaggacggcg
     1201 gcaacagtca gaccggcgaa gcagttacgc atggagtgaa gctcctgttg tgaatgaaca
     1261 gacaaggtaa cagatttgca cgctgttttc gttcgggccg gcggccgctg gcctttacgc
     1321 gccacttttg cgtaagctga tcgcggactc ttgtgagggc aaagaatgcg agctggtcgg
     1381 ttgcggtggt gggcaggaag gatgttgatg gcggcgatga taacgggaac ggcggcggca
     1441 cagccggcga gcagttctca accggggttt gtgaacacgc tcatgccaca accggctcac
     1501 ctgacggcag gtgaaggctg gctgcccctc gcgccgggat tctcggtggg ttttgacaag
     1561 acgcacgatg agcgtctgga ggcggcggtg catcgggacc tccagcgcct cgaggattcg
     1621 accggcgtga tggtccagca tgcgacgcct caagccacgg ctacgctgac gatctccgtc
     1681 gatggcccgg gcgaagaaat tcagggcatc gacgaagacg agtcttactc actcgaaatc
     1741 acaccgcagc acgcgcaact gcacgccgcg accgatgtgg gcgccatgca cggcatggaa
     1801 accctgctgc agctagtgca ggccgacgca cgcggattct ggctaccggc agtctcgatc
     1861 caggatgcac cgcgtttccc gtggcgcggg ctcatgatcg acgtcggccg ccacttcgag
     1921 ccggtgagcg tgatcgagcg cacgctggac gccctggccg ccgtcaagat gaacgtcttc
     1981 cactggcacc tgacggacga tcagggcttt cgcatcgaga gcaggattta tccaaagctc
     2041 accggcatgg gatccgacgg ccagtactac acccaggacg aggccagagc agttgtcgcc
     2101 tatgctcgtg cgcgcggcat ccgcgtcgtt cccgagtttg acatcccggg ccacaccacc
     2161 tcatggatgg tcggctaccc cgacctcgcc agcgctccgg gtccgttttc catcgagcgg
     2221 cacttcggcg tcttcaatcc ggtgatggat cccacgcgcg acagcactta cgaattcctc
     2281 gacaagttca tcggcgagat ggcggacatc ttccccgacg cttacatgca catcggcggg
     2341 gacgagaaca acggcgtcga gtggaagtcc aactcgaaga ttcaggcctt cgcgacggcg
     2401 cacaatctgc acgggacggc ggccatccag gcctatttta atcagcggct gttacccatt
     2461 ctcacgaaac acggcaagaa gatgatcggc tgggacgagg tcctgacgcc ggacctgccg
     2521 aaggacgtca tggtccagtc atggcgggga ttcgactccc ttgccgccgg ggcaaagcag
     2581 ggatacagcg ggatcctctc cgcgggatat taccttgacc acatggatcc cgcgtcggcc
     2641 cactaccgcg tcgatccggt gccggccaac agcgatctga cgcctgaaca ggcagcacgt
     2701 attctgggcg gcgaagcgtg catgtggagc gagtacgtcg gaccgcagac gatagagtcc
     2761 cgtgtctggc cccgtaccgc tgccattgcc gaacgtctgt ggtcgcagcg cacggtcacc
     2821 agcgaagacg acatgtaccg gcggcttgcc gtggagtcgc tccgcctgga acagctcggc
     2881 cttacccaca tctcgcacga gggcacaagc ctgcgtgcgc ttgccggcac cgaggatatc
     2941 gacaccctgc ggcctctgac agcggtgctc gagcccgtcg atttcgacac acgttcgcat
     3001 tggtccgagc acaatggcgt gaccaccctg tcgccattcg acagcctggt cgatgcgctt
     3061 ccccccgatc cgccttcgcg gcacgacttc ggcgatctca tgagcacgta tctgcaggat
     3121 cccgtcggtc atccggagat cgaggcggcg ctcgaacaaa ccttccgctc ctggtcggcg
     3181 gatccacacc gaatggaact tctatccagc tcaccgcggc tcgccgcggc gttgcccagg
     3241 gcacagcaac tggcagaaat gggcacgatg ggcctggagg cgctttcgtt cctttcttcg
     3301 ggactctctg cgccgccagg atggaaagcg cagaagctcg ccgtcctcga cgaagcgcag
     3361 aagccggttg ccctgacgcg ttttacagtc ctgcccgcga tgcgtgcgct ggtcaacgaa
     3421 gtccactaac ttagcctggt cgtgttccgg gccgcagctt cacgttcagg aaccaggacc
     3481 ttcatcgggc ggcaggagct ttcgctcttt catcagctgg aatacgtcct gatgagggat
     3541 ataggcgatg atcttcggtt cggagctacc catgtcaatc aaaaacgaag aacttactgc
     3601 caggtttgta tcgggttgac tctcggggcg gaaattcatc ttccactccg tttcaaccag
     3661 gacgtagcga tccccgaggc gcaggtcacg acgggaggtt agttcggtgg atgtcaggcc
     3721 ggcatcgttg aagagctgtt tgcgagcggg gagcttctgc gcaaaaaccg ctgcaggcac
     3781 ggtcaccggc ccctgcggac cagcagcgag gaagtgttcc gcaaagtggc cggcagcttt
     3841 cgccggatcg tcacttaggc tgtcgcgttc ataggtggaa aagaattgat cgagagcgtt
     3901 ctgggtaaag ggcataagtc agcctccacg gcatagttct gcgtgataac tacctttagt
     3961 attatatcac attttcctgc acaaagcaag gaaggcagct caggcagtcc atcacggagt
     4021 aactgccggc tattctcttc tattcgctac tgagtattgg ctgttgttct ctgcttctac
     4081 aggatccagc caccgccgac cacttcatcg ccgtcatagt aaacggctgc ctgtccgggc
     4141 gtgaccgctc gctgaagatc atcgaagacc gcctcgacct catcctggcc aaccatgcga
     4201 atcgtcgccc acgcaggctc gtgccggtgg cgaatcttcg cccgcacccg catctcgcca
     4261 tcgagcgacg gaatcgagat ccagttcaga tcacgggcac gcaatgtgcg cgtcgttgcc
     4321 tccgcatccg agcccaccgt tacgcggtgc gacgcaggct cgatctggat cacatacaaa
     4381 ggatcgggcg acgccactcc caggcccttg cgctgtccaa tggtgaagtt gtgaatgcca
     4441 ccgtgttcgc caatcacctc acctgtcgtc gaaaccaatt cgcccgcggt gtcgggaatc
     4501 tcctcgccct gctcgtgcag gtaggcgctg atgaatttct tgtagtcgcc gcccggaatg
     4561 aagcaaatct cctgcgagtc gggcttcacg gccaccgcca ggccatgctg cgctgccagc
     4621 gcgcgtactt cgggcttccg ataacttccg agcgggaaga gcgtgcgcga taactgctcc
     4681 tgggtgagcc cgaagaggaa gtaggtctgg tccttcgatt catccacggg gcgcgacagg
     4741 atccagcggt tgcgcgtcgg gtcgtattca ttgcgcgcgt agtgtccggt tgcgatgcga
     4801 tccgcgccga tctggcgggc gcgcaccagg agctgatcga acttcaggtg gttattgcac
     4861 agcgagcatg gaatgggcgt gcggccgttc aggtactcgc gcacaaaggg gcgcacgaca
     4921 tcgtcctcaa atcgctcctg ctcattgacc acgtagtacg ggatatcgag cgcctcggcc
     4981 acgcggcgtg cgtcatacac atcgtcgagc gagcagcaac ggcccgtgac ctgctcgggc
     5041 atgccttctt tgccggcgag ccgccgctgg ttccacagct gcagggtgag cccgacgagc
     5101 gtgtaaccgt cggcacgcag catggccgca acggtcgacg agtcgactcc gcccgacata
     5161 gccacggcaa tggtgttgtt gagggacatg gggaataagg ccctgtcctt agtatgacag
     5221 ggactggccg tccggagagc cgcccttcga cggcctgttc cctattggct gcttgctatt
     5281 ccctattggt tgactccgct cgccaggaac ccgccatcga cgaccagcac ctggccggtg
     5341 acaaaggacg ccgcctccga cgccaaatag atcgccgccc ctaccagctc ttcggtcttc
     5401 ccgaagcgag ccatcggcgt gcgcatcaac agttccttac cgcgcggtgt gttgtcgagc
     5461 agatcggaat tgagcgcggt gcgaaagaca ccgggtgcaa tcgcgttcac cagcacaccc
     5521 ttcttcgacc actctacggc gagcgaccgc gtcagtgacg caaccgcagc tttgctggcc
     5581 gcgtacgctg ccacttcggc gagagccaca tacgagttca gcgaagcaat gttgatgatg
     5641 cggccgtagc cgcgctccag catgtgggcg ccgaagattt ggcaggcgcg cagggttccg
     5701 gtgagattcg tgtcgacaat atcgcgccac tcgtcttccg ggaacgtgag ggtgggcgcg
     5761 cgcttgatct ttcctgcact gttgacgagg atgtcgacct tgccgaatgt cttcaccgat
     5821 tcgtccagca atcgctgcaa cgagccgcga tcctgggtgt cgcaggtcag gcgaagacat
     5881 ttgcgacccg tcgtctcgat tgcagcagcg gtctcgtcca cctgttcctg gcggcgcgcc
     5941 gaagcgacca cgtcggcgcc ggattccgcc aggccaatcg agatcgctcg accgatcccc
     6001 gaggttccgc cgacaacgac agccgtcctg ccatcgagct gaagagccgg aatactcatt
     6061 ggacctccgc gtgggccaga gctgcggccg gcgatggctt gcccaggaac acaggcgcga
     6121 tgtggcgatc gtacagattc tcagtcacgt ttttggcaat cactttctca ttggattcaa
     6181 gcaaggcgat gtaacgatcg cccattttcg cagcgatctt aaagcctacg tgcaacagct
     6241 ggcgcaggct cgggttgtaa gccggcgaat gcgggtcgtg ccgcagcgcc gacgtgaact
     6301 gcgccgaggt ccactgcgca accaccgatg gcgcaggcaa cttcgccgga tcgatgtcga
     6361 tcaccgtcgc gtagggtcca caaagctcct cgcgatgctc gtatgcctgc gcgtagattt
     6421 ccttcgcgag ttcgaggccg gcaccaccgg cttcagccag cccgatgatt tcttcgagcc
     6481 aggttgttcc cgcggttttc agatgaacac ccgactcttt cgtggcgtca tgaatcgccg
     6541 catagatcga aaacttgtcg ctgcccgagt gaacgctcag cttcagattc ttcggcaacc
     6601 caagatttgc agtcgcccag gcgatcgctg ccacgtccag aatcatttcg cgcctgaact
     6661 gatcgacgtt gcccacataa tcgacgcctt tgttgaaccg gccggtgaat ttcggcgcga
     6721 tggtctggat cggaatcccc tcatcggcga tcgcggccag gatgatgagc agttccgcgg
     6781 gagtctgcgc ggcgtcggtt tcgtccatcg aaacctcggg aacgaaattc cccttccctt
     6841 tcaatccttc aatcttgcgg taaaccttgc ccgcctcctg cacggcaaag aggaacttag
     6901 tagccgtctc gcgcagcagt tccggcgtga tctggatggg ttctctgacc ccttcgagcc
     6961 ggaatgctcc tttcagatcg gagcgccgct tcaggaatgc ctcgatcgac gccggctcgg
     7021 ccggcttgcc gatgtagtcc gccacatcga tggtgaagaa gtcgcagggc gccagaaacc
     7081 ggtccaccgt cgtcaggttg atgtggtcgg catcacagaa gtaaggcttc gtccacccca
     7141 gatccttcac tgccgcatcg gcagcggcac gggtcgagga cggctccgaa ccgataatgt
     7201 tgtgctcgcg gtttgatttg ttccagaccg gaatcacctc gatgcccgag cggccggctt
     7261 caatacaagc ctgcagttgc gccttcgcct gatgagcgaa ccggtcgcca accccaatag
     7321 aaaattttcc gagttccgtc gttgccataa gtacctcagt ctttaaatcg atcgcgatgg
     7381 gcccagagtc ccaggcccgg gtttgcgacg aagaacacct cttcgccttc cacattgtta
     7441 aaaccgtgct tcagctttgc
//
LOCUS       DASZYN010000095         6070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_644665, whole genome shotgun sequence.
ACCESSION   DASZYN010000095 DASZYN010000000
VERSION     DASZYN010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644665"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..491
                     /locus_tag="VHE33_02525"
     CDS             264..491
                     /locus_tag="VHE33_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96352.1"
                     /translation="MPASQAHLYAPSDKIMICGEELSMSPRPITENEGVLRANILLAG
                     TESGKASSKSDEHQMDAISNGIDFLGDWLLR"
     gene            complement(477..551)
                     /locus_tag="VHE33_02530"
     tRNA            complement(477..551)
                     /locus_tag="VHE33_02530"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Asn,seq:gtt)
     gene            complement(615..1397)
                     /locus_tag="VHE33_02535"
     CDS             complement(615..1397)
                     /locus_tag="VHE33_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HVT96353.1"
                     /translation="MTCHRIFRVKLVRVFCGVLALALLGTVPPTHAQSGDDAHRIFDL
                     TNQDRREHGLPLLRWDASLAHAARSHADRMARERALSHRYSDEPELTERGASAGAHFR
                     AIAENIATGPDAGAIENEWMHSPPHRANILDPKMDALGVAVVEHGGRLYAVEDFEQSS
                     QALTKEQVEGKVRELLHARGVDPSADAGPAEQACASGHGVPQGTNARSIVRFDTPDLS
                     QLPAQVVEVIRSKDFKRAAVGACAPNSGQANFTTYGVAILFY"
     gene            complement(1369..2415)
                     /locus_tag="VHE33_02540"
     CDS             complement(1369..2415)
                     /locus_tag="VHE33_02540"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT96354.1"
                     /translation="MAASSVSAGEGTVVLFGGTGFIGSHLAMHWLQEKLAVKVILVDL
                     NPPRIEPWTLALQQELKAGRAEFVRWDVRQPLPETLLPKAGLIFNLAAVHREPGHEAH
                     EYFETNLKGAENVCAWAAATGCNRIVFTSSIAPYGPSEEMKDEASLPVPDSPYGSSKL
                     AAEKMHMAWQAGCDCRKLLILRPGVVFGPGEGGNVTRLIRSVVKGYFVYMGNRQTRKA
                     GSYVKELSRVIEFGLDRQDASGEPVTLLNVSLDPPPTLEEYVAAIQKAAGIRRSPLQL
                     PRALLLGVSWPIHTAASLFGIHQPISPVRVRKLFRSTSIDPKGLRELGYKYHYTLETA
                     LLDWKNDLPQDFSR"
     gene            complement(2397..3815)
                     /locus_tag="VHE33_02545"
     CDS             complement(2397..3815)
                     /locus_tag="VHE33_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVT96355.1"
                     /translation="MGAVDPPEGWTGQNLRVDRKPDAGRSRPLIAASEQPISGLREDG
                     QARPMRIFVNEFCGHPFQMELSRRLAKMGHVVCHVYFADNQSTPKGATQRRPDDSPDV
                     AIEGLHIQREFSKHSLRTRRQADLEYGREAAKRMAQFAPDVVISANMPLDAQNLLQQA
                     AREQGARFVFWLQDVYSIAVRFVLNRKAKPLAAAGGWYFERLEKKLLRQSDAVVCIAP
                     GFAEFVRGWGVPESKIAVIRNWAPLEEVIPTPRDNAWASENGFDGRFRFMYSGTLGMK
                     HRPELLLDLAKHLDVRKDARLVVIAGGAGADWLKERAHEVSPETLSVLPFQPYERISE
                     VMGSADVLIALLDSEAKTFAVPSKTLSYLCAERALIVAAPAANEAARVVEEAQAGLIV
                     SPDDASGIVSAAERFLEDRTFLAQCATNARRYAEQHFSIDTIAGRFLEVLQPGSEKPE
                     LDAEKMVLTNGGERKPSWQRPA"
     gene            complement(3728..4717)
                     /locus_tag="VHE33_02550"
     CDS             complement(3728..4717)
                     /locus_tag="VHE33_02550"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVT96356.1"
                     /translation="MKLKGRKVAVCGGGGFIGGHLVRYLRTQGVDVVRAVDVKPVDEW
                     YLVSDDVENLVLDLKEKDNCHKAAKGMQGIFQLAADMGGMGFIENNKALCMLSVLTNT
                     HMLMAARDAGVERFFYSSSACVYNGEKQKDANVTPLKEADAYPALPEDGYGWEKLFSE
                     RMCRHFEEDFGLICRVARYHNVYGPHGTWTGGREKAPAAICRKVIEAKMSGSHEIEIW
                     GDGKQTRSFMYIDDCTRGTLAIAESDIDEPINLGSSELVTINQLVDIVEEIAGVKLKR
                     RYKLDAPKGVNGRNSDNAMILEKLRWEPSIRLKDGLAKTYEWIESQMPAGAGR"
     gene            4964..5839
                     /locus_tag="VHE33_02555"
     CDS             4964..5839
                     /locus_tag="VHE33_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT96357.1"
                     /translation="MALPAGSTGRRSRMWASLRRQPLAAIGVVLMVSFTIMALLAPWL
                     APYNPSALDLAHRLAPPGGAHWLGTDELGRDILSRVIFGARLSLTVAVSVVGLSFFLG
                     LIFGGIAGYYGRWLDVGINTFLMNAFLALPGILLAIAFVAFLGPGLLNLILALSIGGW
                     VGYARLVRAQVLAVREREFVEAARALGASDLRIFTRHILPNILQPLIVQAAIGMAGAV
                     LAEATLSFLGLGVPPPAASWGSMLNDARPFLFEAPHLVVFPAIAVMLCVLSFNFLGDA
                     LRDYLDPRTRIAVGL"
     gene            complement(5840..>6070)
                     /locus_tag="VHE33_02560"
     CDS             complement(5840..>6070)
                     /locus_tag="VHE33_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96358.1"
                     /translation="PVENALQLALPPRKADDAVERWRGQAVRPRVAVAILAVIFLGVI
                     LFARMSGHWQTQVPKHVYMMLVPNANQVSHPM"
BASE COUNT         1091 a         2021 c         1735 g         1223 t
ORIGIN      
        1 gacggtgtgc gaccagcagc ccgccacctg cgatttccgg cagtggttgc gaagagtcag
       61 cccgatttgc ctgcagatga tcgcagacgg caacaaaacg atgccgcatg ccggctgacc
      121 ccggctctcg acaacttcaa aggttgtaga cgggtagcgc gcaggaaaat aatgctgcca
      181 taggcgctgc tccgtccgca ttcaccacgg acctgccccg tgcaagtcgg cgtctcttgc
      241 catggcgctg tctgaaatta caaatgcccg ccagtcaggc acatctctat gccccttcgg
      301 acaagatcat gatttgcggt gaagagctgt ccatgtcccc caggcccata acagaaaacg
      361 aaggagttct tcgcgcgaac atcttgttag caggcaccga atccggcaaa gcttcgtcaa
      421 agtccgatga gcatcaaatg gatgccatct ccaacggaat tgatttttta ggggattggc
      481 tcctcaggta ggactcgaac ctacaaccct tcggttaaca gccgaatgct ctgccattga
      541 gctactgagg agtatgccgt ccgactccga gattatatca aaccgcgcgg ccgccgcagc
      601 ctgaccccgc cgcctcaata gaagagaatt gccacaccat aggtcgtgaa gttcgcctgg
      661 ccagagttcg gcgcacacgc tcccaccgcg gcccgtttga agtccttgct gcgaatcact
      721 tcgaccacct gcgccggaag ctggctcagg tcgggtgtat cgaaccggac gatggatcgc
      781 gcgttcgttc cctgtggaac tccgtgaccg ctcgcgcacg cctgctccgc cggaccggca
      841 tcggcagagg ggtccactcc gcgcgcgtgc aggagttctc gcactttccc ttccacctgc
      901 tccttcgtga gtgcctggga cgactgctcg aaatcctcga cggcgtacag tctgccgcca
      961 tgttccacga ccgcgacgcc caacgcgtcc attttggggt cgagaatgtt ggcgcggtgc
     1021 ggcggtgagt gcatccactc gttctcaatt gccccggcgt cgggccccgt tgcaatgttc
     1081 tcggcaatgg ctcgaaaatg cgctcccgcg gacgctccac gctcggtaag ctcaggttcg
     1141 tcggaatacc gatgcgagag tgcccgttcc cgcgccatgc gatctgcatg gctccgcgcc
     1201 gcatgagcca gagaggcgtc ccagcgcaac agtggcagcc cgtgctcccg ccggtcctgg
     1261 ttcgtgagat cgaagatgcg atgcgcatcg tccccggact gcgcgtgtgt cggcgggacc
     1321 gtcccgagca gagccagagc gagcactccg cagaaaacgc ggacgagttt aacgcgaaaa
     1381 atcctgtggc aggtcattct tccagtccag gagcgctgtt tccagcgtgt agtgatactt
     1441 atagccgagc tctcgcagac ctttgggatc gatcgaggtg gagcgaaaga gtttgcgaac
     1501 ccgcaccggg ctgatcggct ggtggattcc aaacaggctt gctgcggtgt gaatcggcca
     1561 ggagactccc agcagcaggg ctcgtggcag ttgcagcggc gatcgccgga tccctgctgc
     1621 cttctggatc gccgcaacat attcctccag cgtcggcggc ggatcgagag agacattcag
     1681 tagcgtgaca ggctcgccgc tcgcgtcctg gcgatcgaga ccgaattcga tgactcggct
     1741 gagttccttc acatagctgc cggccttccg cgtctgccgg ttccccatgt aaacgaagta
     1801 gcctttcacg acactgcgaa tcaggcgcgt gacgttgccc ccctctccag gcccgaagac
     1861 aacccccgga cgaagaatga ggagcttgcg gcagtcgcag ccggcctgcc aggccatgtg
     1921 catcttttcg gccgccagct tggaactccc atagggtgaa tccggtacgg gcagcgatgc
     1981 ttcgtccttc atttcctcgg aaggaccata aggggcgatg ctgctggtga agacgatccg
     2041 gttgcatcca gtcgccgcgg cccaggcgca aacattctca gcgcccttca ggttcgtttc
     2101 aaagtactcg tgcgcctcgt gtcctggctc acgatgcacc gcggcgagat tgaagatgag
     2161 gcccgctttc ggaagaagcg tttccgggag aggctggcga acatcccatc gcacgaactc
     2221 ggctcgaccc gccttcagtt cctgctgcag cgcgagggtc catggctcga tgcgcggtgg
     2281 gttcagatcc accaaaataa ctttgacagc cagcttctcc tgaagccagt gcatggccag
     2341 gtgggatccg atgaagccgg tcccgccgaa cagtacaacg gtaccctcac cggcgcttac
     2401 gctggacgct gccacgatgg cttccgttcg cctccgtttg tgagtaccat cttctccgcg
     2461 tcgagctcag gcttttcgga acctggctgg agcacctcca gaaagcgacc ggcaatggtg
     2521 tcgatggaaa agtgctgttc cgcatagcgg cgggcattcg tggcgcattg agcgagaaag
     2581 gtgcggtcct cgaggaaacg ctccgctgcg ctcacaatgc cgctcgcatc gtcgggcgag
     2641 acgatgagtc cggcctgcgc ctcttccacc acccgcgctg cttcgttcgc tgccggagcc
     2701 gcgacgatca gcgcccgctc cgcgcaaagg taggagagag ttttggacgg aacggcaaac
     2761 gtcttcgcct cggaatccag cagagcgatc agcacgtcgg cggaacccat gacttcggaa
     2821 atccgctcgt agggctggaa cggcagcacg ctcagcgtct cggggctgac ctcgtgcgct
     2881 cgttccttca gccagtctgc gcccgctcca ccggcaatca ccaccagtcg cgcatccttg
     2941 cgtacgtcga ggtgctttgc cagatccagc aacagttcgg gacggtgctt cattcctaac
     3001 gtgccggagt acatgaagcg gaagcggccg tcgaatccgt tctcgctcgc ccaggcgttg
     3061 tcgcgaggcg tgggaatcac ttcctccagt ggcgcccagt tccggatcac agcaatcttc
     3121 gactccggca cgccccaccc gcgaacaaac tcggcaaacc cgggagcgat acaaactacg
     3181 gcatccgact ggcgaagcag cttcttctcc agccgctcga aataccatcc gcccgccgcg
     3241 gccagcggct tcgccttgcg attgagcacg aatcgaaccg cgatggaata cacatcctgc
     3301 agccagaaca cgaagcgcgc gccctgctcc cgcgccgcct gctgcagaag attctgcgca
     3361 tcgagaggca tgttggcgga gatgacgaca tccggtgcga actgcgccat gcgcttcgcc
     3421 gcctcgcggc catactcaag atccgcctga cgtcgcgttc tcagggaatg cttcgaaaac
     3481 tctctctgga tgtgcaggcc ctcgatcgcc acatccggag agtcatccgg ccggcgctgc
     3541 gttgcgccct tcggcgtcga ctggttgtcg gcgaaataga cgtgacacac tacatggccc
     3601 atcttcgcga gtctcctgct gagctccatc tgaaatgggt gcccgcagaa ttcattgaca
     3661 aagatgcgca ttggcctggc ctgcccgtcc tctctcaatc cggaaatcgg ctgttccgac
     3721 gccgcgatca gcggcctgct cctgccggca tctggctttc gatccactcg taggttttgg
     3781 ccagtccatc cttcaggcgg atcgacggct cccatctcag cttctcgagg atcatcgcgt
     3841 tgtcgctgtt gcgcccgttt acgcccttcg gcgcatccag cttgtagcgt cgtttcagct
     3901 tcaccccggc gatctcttcc acaatatcga ctaactggtt gatcgtaacc aactcgctcg
     3961 agcccaggtt gatgggttca tcgatatcgc tctccgcgat ggcgagggtg cccctcgtgc
     4021 agtcgtcgat atacatgaag ctgcgcgtct gcttcccgtc accccagatc tcgatttcgt
     4081 gactgccgga catcttcgct tcgatcacct tccggcaaat cgccgccggg gccttttcgc
     4141 gtccgccggt ccacgtgccg tggggcccgt aaacattgtg gtagcgcgcg acgcggcaga
     4201 tcaggccgaa gtcctcttca aagtggcggc acatgcgctc actgaacagc ttttcccacc
     4261 cgtagccgtc ttcaggaagc gccggatacg catcggcttc cttcagcggc gtcacgttcg
     4321 catctttctg cttctcgccg ttgtagacgc aggcggagga agagtaaaag aagcgctcca
     4381 caccggcgtc tcgcgcggcc atcagcatgt gcgtattggt aagcacgctc agcatgcaca
     4441 gggctttgtt gttttcgatg aagcccatgc cacccatatc ggccgccagc tggaagatgc
     4501 cctgcatgcc cttcgcggct ttgtggcagt tgtccttttc cttcagatcg agaaccaggt
     4561 tctcgacgtc atccgatacg agataccact cgtcgacagg tttcacatcg acggcgcgca
     4621 caacatccac gccctgggtc cgcagataac gcaccaggtg accgccaatg aagccaccgc
     4681 caccgcaaac ggcaaccttc cggcctttca gcttcattgc gactccagac gatctctcga
     4741 aacggaaatg agagccgtgc gagattgagc atggcccgta cagcccgcac cagcctgcct
     4801 gcgaacggtc tccgcagggc cgtttcctgg aacgattcta ttcgacgggt ttacaaattg
     4861 atataaccca taggggagcc gctgccgccg cgcggcgtca ccggttgcat catcctgaga
     4921 cacgcgccgc caaaaactct tgctcctcaa cgctgacaat tcgatggcac tcccagccgg
     4981 ctcgacggga cgccgttcca ggatgtgggc ttcgctgcgt cgccagccgc tggccgcaat
     5041 cggtgtcgtt ctgatggtct ccttcaccat catggcactg ctcgctccct ggcttgctcc
     5101 ttacaacccc tccgctctgg acctggctca ccgccttgcc ccgcccggcg gcgcgcactg
     5161 gctcggcacc gatgagttgg gccgcgacat tctctcccgg gttatcttcg gcgcacggct
     5221 ctcgctcacc gtggcggttt cggtggtcgg tctgtcgttc ttcctggggc tgatcttcgg
     5281 cggcattgca ggctactacg ggcgctggct cgacgtcggc atcaacacct tcctgatgaa
     5341 tgccttcctg gccctccccg gtatcctgct ggcgattgcc ttcgtggcgt tcctgggacc
     5401 gggactgctg aacctgatcc tggccttgtc catcggcggg tgggtcggct atgcgcggct
     5461 ggtgcgagcc caggtcctgg ccgtgcgcga gcgcgagttc gtggaggccg cccgcgccct
     5521 cggcgccagc gatctgcgca tcttcacgcg gcacatcctg cccaacatcc tgcaacctct
     5581 catcgtgcag gcggcgatcg gcatggccgg agccgtcctc gccgaggcca ccctcagctt
     5641 cctcggactc ggcgttcctc cgccggccgc gagttggggc tccatgctga acgatgcacg
     5701 gcccttcctc ttcgaggcgc cgcacctggt cgtcttcccc gccatcgcgg tcatgctgtg
     5761 tgtgctgtcg ttcaacttcc tcggcgacgc gctgcgcgac tacctcgatc cccggacccg
     5821 catcgccgtc ggcctctagc tacatcgggt ggctgacctg gttggcgttc ggcaccagca
     5881 tcatgtacac atgcttcggc acctgcgtct gccagtggcc gctcatgcgc gcgaacagga
     5941 tcacccccag gaaaatcacc gccaggatcg ccacagcaac acgcggacga accgcctgcc
     6001 cgcgccagcg ctccacagca tcatcggcct tccgcggcgg cagggccagt tgcagcgcgt
     6061 tctccaccgg
//
LOCUS       DASZYN010000096         5591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_68951, whole genome shotgun sequence.
ACCESSION   DASZYN010000096 DASZYN010000000
VERSION     DASZYN010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68951"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VHE33_02565"
     CDS             complement(<1..376)
                     /locus_tag="VHE33_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVT96359.1"
                     /translation="MPGAKRLTLSQTRGPDRPLLNLTIGELLDRTASSFPDRDAVVSC
                     HQGQRLTWAELSEQADRVARGLWALGIRRGDRVGLWSTNCVEWVMMHMGTARAGASLV
                     NVNPAYRSHELQYTLTRSRMKAL"
     gene            complement(477..986)
                     /gene="lspA"
                     /locus_tag="VHE33_02570"
     CDS             complement(477..986)
                     /gene="lspA"
                     /locus_tag="VHE33_02570"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HVT96360.1"
                     /translation="MNGRVKRDRRAWLLAIAALVILFDRLTKLWIIHHVEEGDAIVVV
                     QRVFRITHVRNPGAAFSMFTESTRPEMVRWVLSGFSILAAAVVLFAILRFGRRMTPTM
                     IALALILGGTIGNLWDRLRSGLVTDFLEVHIIHYHWPDFNVADSAIVIGGILLVLDAL
                     FSGTKEPSS"
     gene            complement(983..3946)
                     /gene="ileS"
                     /locus_tag="VHE33_02575"
     CDS             complement(983..3946)
                     /gene="ileS"
                     /locus_tag="VHE33_02575"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HVT96361.1"
                     /translation="MSSAPPSGDPKPVALKDTLSLPRTGFPMKANLPQTEPARLEAWE
                     RSRLYEQIRAARQGAPRWILHDGPPYANGPIHLGHALNKCLKDFIVKCKTMAGFDSPY
                     VPGFDCHGLPIEIKVDEKLGRKKLEMPAAEFLRQCREYAQKYVDLQTSQFVRLGVFGR
                     WDNPYSTMSRSYEARILETFYRFLEGGFVYRGLRPVYWCIHDRTALADAEIEYEQHSS
                     PSIYVRYDLTSDPAAIHPALAGKKVATIIWTTTPWTLPASLAVAFHPDFDYVALEQAG
                     QVYIVAQALASPVREACDLEAAVPIAGFKGSRMERATFRHPFLDRQILGVIADYVTAD
                     QGTGAVHTAPAHGADDFYTGARYGLDQSCHVDNAGRIRDGLPEYDGKTVFQANQPIID
                     LVASRGALLGRSDIFHSYPHCWRCHHPVIFRATEQWFINIDHPMPLAGGPNGAPVTFR
                     QRALDEIRKSVKWDPAWGEERIANMIATRPDWCISRQRVWGVPIAVFLCDGCHQPLMD
                     RGLNQRILELFEKEGVEVWHTKAADALLPDGAHCAKCGGTAFRRETDILDVWFDSGSS
                     WHAVLEAEPGLAPPADLYFEGGDQYRGWFHTSLLTSVGVGQKHETPFRMVGTAGWTLD
                     EQGRAMSKSLGNGVDPVDIADRLGAEIVRLWVASVDFREDVVNSEHIMQRLADNYRKL
                     RNTFRFLLGNLDGFNPVSDSVSDTELLPLDRYMLTRSRELVEKVSGTDGKGGWYGDFQ
                     FHRVYHAINEFCIVDLSAFYLDVLKDRLYTFAPKGLERRSAQTVLWRIAEALVRLVAP
                     ILTFTSDEVWQYLPAVSDRPDSVHLTLFPSAGELGAADSHLSADWQRLLAIRDKVLLA
                     LEAERKAGKIGKALESSVHIEYSTFAGKPVGDGALLLRYEPALKELFNVSSVRLVSRG
                     EHEAGAVPIGDPEALSQLIRIVVVAAEGTRCDRCWNYYADDGPQHVRNYGAWKNVCGR
                     CAAALREMGFNEDGR"
     gene            4240..5181
                     /locus_tag="VHE33_02580"
     CDS             4240..5181
                     /locus_tag="VHE33_02580"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HVT96362.1"
                     /translation="MHLGGLDSIVEEPIWKTLFNSVVDVLFPKKLPPLKLSSTPIAVR
                     DPMAMKRSPASSAVSFITHVVVIGLILLFTIEQWHERQVAKKAVVTPVDISPYVPPSP
                     LKDTMGGGGGGGNHELVEASKGKLPPMVKKPLAPPQILKIDNPKLAVTPAVLMPQQIK
                     LPDNPNMPNLGVPQSPQIALASQGSGSGSGFGSGSGGGIGSGSGNGVGPGSGGGYGGG
                     IYHPGGGVSNPVLVYAPDPEFSDEARRAKYQGVCVVGLIVDAQGNPQRVRVVRPLGMG
                     LDEKALEAVRQYKFKPATYQGKAVPVEINIEVNFRIY"
     gene            complement(5260..>5591)
                     /locus_tag="VHE33_02585"
     CDS             complement(5260..>5591)
                     /locus_tag="VHE33_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HVT96363.1"
                     /translation="ARGRTRDGRDLFQPETLDLFAQRQESNTGPSRALGWDVPTAGSS
                     SGRYFGPRSIGHLGYSGCSLWIDPDQDLAVALLTNRTWPDRSNQAIRQVRPAFQDAVV
                     ETLRSSS"
BASE COUNT         1128 a         1708 c         1701 g         1054 t
ORIGIN      
        1 agagggcctt catgcgtgac cgcgtgaggg tgtactgcaa ttcgtgcgag cggtaggcag
       61 ggttcacgtt gaccagggat gccccggcac gggctgtgcc catgtgcatc atgacccact
      121 cgacgcagtt ggtcgaccaa agtccgacgc ggtcgcccct gcggattccg agcgcccaaa
      181 gaccgcgcgc cacgcgatcg gcctgctcgc tcagttccgc ccatgtcaga cgctgtccct
      241 ggtggcatga gaccacggcg tcgcgatccg gaaaggagct cgcggtgcga tccagcaact
      301 cgccaatggt caggttcagg agggggcggt caggtccacg ggtctgactc agtgtcagac
      361 gtttggcacc aggcaaggtc ggtgttacca acacggttgc agtctacgcg aagggaaaac
      421 ccagagatgt gacgaagtgc acagaggcgc actcgccacg gaggacgcga gtgcgttcag
      481 gagctgggtt ctttcgtgcc ggagaacagc gcatccagca ccagcagaat tccgccgata
      541 acaatcgctg aatccgccac attgaagtcg ggccagtggt agtggatgat atggacttcg
      601 aggaagtcgg tcaccagacc tgaccgcagc cgatcccaca gattaccgat ggtgccgccg
      661 aggatgagcg ccagcgcaat catcgttggt gtcatgcggc gcccaaagcg caggatcgcg
      721 aatagaacca cggccgccgc caaaatggag aagcctgaca gcacccaacg gaccatctcg
      781 gggcgcgtgg attcggtaaa cattgaaaac gctgccccgg gattccgaac gtgggtaata
      841 cgaaagacgc gctggaccac gacgattgca tcgccctcct cgacgtggtg gatgatccag
      901 agtttggtca gacgatcgaa gaggatcacg agagccgcaa tcgccagcag ccacgctcgg
      961 cggtcccgct ttactctccc attcatcggc cgtcctcgtt gaagcccatt tcccggagtg
     1021 cggcagcaca tcgaccgcac acattcttcc aggcgccata gttccgcaca tgctggggac
     1081 catcgtcggc gtagtagttc cagcagcggt cgcaacgcgt tccttcagcc gcgaccacaa
     1141 cgattcgaat cagctgggac agggcttccg gatcgcctat gggtaccgca cccgcctcgt
     1201 gttctcctcg actgactaac ctcaccgagg agacattgaa cagctctttg agtgccggct
     1261 catagcgcag cagcaacgcg ccgtccccca ccggtttgcc ggcaaaagtc gagtactcaa
     1321 tgtgaacgct ggactccagc gccttgccga ttttccccgc cttgcgttcc gcttccagcg
     1381 ccagcagaac cttgtcccgg atagccagca gccgctgcca gtcggcagac aagtgggaat
     1441 cagctgcgcc cagttcgccg gcactgggaa acagcgtgag atggacgctg tccggcctgt
     1501 ccgacacagc gggcagatac tgccagacct cgtccgaggt gaacgtcagg atcggcgcta
     1561 caagccgaac aagcgcctcg gcgatcctcc acagcacagt ctgcgcagat cggcgttcca
     1621 gccccttcgg tgcaaaggtg tagagccggt ctttcagcac gtccagataa aacgcgctca
     1681 gatcgacgat acaaaactca tttatggcat ggtaaacacg gtggaactgg aagtcgccat
     1741 accagccgcc cttcccatcc gttccggaaa ccttctccac caactcgcgg gaacgggtaa
     1801 gcatgtagcg atccagcggc agcagctccg tgtctgacac ggagtctgac acgggattga
     1861 atccatccag attaccgagg aggaaacgaa acgtattgcg cagcttccgg taattgtccg
     1921 ccagccgctg catgatgtgc tcgctgttga cgacatcctc gcggaagtca acgctggcca
     1981 cccaaagacg cacaatctcc gcgccgaggc gatcggcgat gtccaccgga tcgacaccat
     2041 tgcccagcga tttcgacatg gcgcgcccct gttcgtccag cgtccagccc gccgtcccca
     2101 ccattcgaaa aggggtttcg tgcttttgtc cgacgccgac cgacgtcagc agggaagtgt
     2161 ggaaccagcc gcgatactga tcgccgcctt caaagtacag gtcggccggc ggcgccagcc
     2221 ccggttcggc ttcgagcacc gcatgccagc tggagccgga gtcgaaccac acgtccagaa
     2281 tgtcagtttc tcgccggaaa gcggttccgc cacatttcgc gcagtgtgcg ccgtccggca
     2341 gcagcgcgtc ggcagccttc gtgtgccaaa cttccacacc ttccttctca aaaagctcca
     2401 ggattcgctg gttcaggccg cgatccatca gcggctgatg acaaccatcg caaaggaaaa
     2461 ccgcaattgg cacgccccat acacgctggc gcgagatgca ccagtccggc cgcgtcgcaa
     2521 tcatgtttgc gatgcgctct tccccccacg ccgggtccca tttcacgctt tttctgattt
     2581 cgtcgagcgc gcgctggcgg aaggtgaccg gtgcaccgtt tggaccaccc gccagcggca
     2641 tcgggtggtc gatgttgatg aaccactgct cggtcgcccg gaagataacc gggtggtggc
     2701 agcgccaaca gtgcgggtag ctgtggaaga tgtccgaacg gcccaacaga gcgccgcggc
     2761 tggctaccag atcgataatc ggttggttag cctggaagac ggtctttccg tcgtactcgg
     2821 gcagaccatc gcgaatccgt cccgcgttgt cgacgtggca gctctggtcc aatccatagc
     2881 gggccccggt ataaaagtcg tctgcaccat gtgctggcgc cgtatggaca gcgccagtcc
     2941 cctgatcggc cgtgacgtaa tccgctatga ctccaaggat ttgccggtcc aggaatggat
     3001 gccgaaaagt tgcgcgttcc atgcgcgaac ctttgaaccc ggcgatcggc accgccgctt
     3061 ccaggtcgca cgcttcacgc acgggcgacg cgagagcctg ggccacaatg tagacctggc
     3121 ccgcctgctc cagggccaca tagtcgaaat caggatgaaa cgcgacggcg agcgatgccg
     3181 gcagcgtcca gggggtcgtc gtccagatga tcgtcgcgac ttttttgcca gccagcgccg
     3241 gatggattgc cgccggatcg ctggtcaggt cgtagcggac ataaatactg gggctggaat
     3301 gctgctcgta ctcgatttcg gcgtccgcga gggcagttcg gtcgtggatg caccaataca
     3361 cggggcgaag tccccgatag acaaatccgc cctcgaggaa gcggtagaac gtctcgagga
     3421 tgcgcgcctc gtagctgcgc gacattgtcg agtaggggtt gtcccagcgg ccaaatacgc
     3481 ccaggcgcac gaactggctg gtttggagat cgacatattt ctgggcgtac tcgcggcact
     3541 ggcgcaggaa ttcagcggcc ggcatttcca gctttttgcg ccccagcttc tcatctactt
     3601 tgatttcaat tggaaggccg tggcagtcga accccgggac gtagggcgag tcgaacccgg
     3661 ccatcgtttt gcacttgaca atgaagtctt taaggcactt attgagcgca tggccgaggt
     3721 ggatggggcc attggcatag ggtgggccat cgtgcaggat ccagcggggc gctccctggc
     3781 gggccgcgcg gatctgctca tacaagcggg atctctccca tgcctccagc cgggcgggct
     3841 cggtttgcgg cagattggcc ttcataggaa agccggttcg gggcagagag agggtatctt
     3901 tcagggcaac gggcttaggg tcgccggatg gcggcgcaga cgacatcggt tctccagctg
     3961 gtttcgggta aaagttcatt gtatccggcg ggacagcagt aacttcctga cagatcaagc
     4021 gtctatcgag gaagtgagat gtgcaggtga ttttttcggg gtgggccgca tctcagcaag
     4081 ggcagcaacg ctccagacgc gacacagagg gcggctgtca ttgataatca ggagaagccg
     4141 gccggaaacg cccggcgagc caacatgggg aataccgttt tgacgtctcc gcacctggta
     4201 ccagattcaa atcggggtag atatcaagag ccgccagcat tgcatctggg gggtctcgat
     4261 tcgatcgtcg aagagccgat ctggaagacg ctcttcaaca gcgttgtcga cgtgctcttc
     4321 ccaaagaagc tgccgccgct caagctgagt tccacaccca ttgccgtccg cgatccgatg
     4381 gcgatgaagc ggagcccggc atcgagtgcg gtttcgttca tcacgcacgt cgtcgtgatc
     4441 gggctcatcc tgctcttcac gatcgagcag tggcacgagc ggcaagtggc gaaaaaggct
     4501 gtagtcacgc cagtcgacat ttctccgtat gtacctccgt ccccgctgaa agacacgatg
     4561 ggcggtggcg gcggcggcgg caatcacgaa ctggtagagg cttccaaagg caagctgcct
     4621 ccgatggtca agaagcccct cgcgcctcct cagattctga agatcgacaa tccgaaactt
     4681 gctgtcactc cagcagtgtt gatgccgcag cagatcaagc tgcctgacaa cccgaacatg
     4741 ccgaacctcg gcgttccgca gtcgccccag atcgcgctgg cctctcaggg ttccggttcc
     4801 ggctccggtt tcggaagcgg ttccggtggc ggtatcggct caggcagcgg caatggcgtc
     4861 gggccaggct ccggcggtgg atatggcggc ggtatctatc atccgggcgg cggcgtttcc
     4921 aaccctgtcc tggtctatgc gccggatccg gagttctccg atgaagcgcg gcgtgccaaa
     4981 tatcagggtg tttgcgtagt gggcctcatc gtggacgcgc agggcaatcc gcagcgcgtg
     5041 cgcgtggtgc ggccgctggg catgggtctg gacgagaagg cgctggaagc cgtgcggcag
     5101 tacaagttca agcccgccac ttatcagggc aaggcggttc cggttgagat caacatcgag
     5161 gtcaacttcc gcatctatta gtcttctgtt cgaaagcaaa agccttcgct gccattcggc
     5221 cgcgaaggct tttttgtgcg ccacacgtcg catccctgtt cacgaactgc tgcgcagtgt
     5281 ttccacgacc gcatcctgga atgccgggcg tacctgcctg atcgcctgat ttgagcgatc
     5341 cggccaggtc cgattggtca acagagcgac cgccagatcc tgatcggggt cgatccacag
     5401 cgagcacccc gaatagccga ggtgtccgat ggaacggggt ccaaagtacc gtcctgaaga
     5461 cgaacccgcg gtgggcacat cccatccgag cgctctcgac ggtccagtgt tcgactcctg
     5521 cctctgcgcg aacaaatcca gggtctccgg ttggaagagg tcgcggccgt cgcgggtgcg
     5581 acctcgtgcc a
//
LOCUS       DASZYN010000097         1710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_4675, whole genome shotgun sequence.
ACCESSION   DASZYN010000097 DASZYN010000000
VERSION     DASZYN010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4675"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..>1710
                     /locus_tag="VHE33_02590"
     CDS             316..>1710
                     /locus_tag="VHE33_02590"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HVT96364.1"
                     /translation="MQIDLRAGSKISDTSGMSQEPLITEELIARQPPESQAIIRLLLA
                     KIAELEARLNKTPRNSSLPPSIEHPHAKATPKKRKSGRKRGGQPGHPKHERSLIPVEQ
                     CTQVVPAIPENCRRCGEVLAGLDSEPLRHQVWEIPEIKPLVTEYQLHRLMCPACSTST
                     CGQLPPGVPHGQAGPRLIALTGLLMGCFKQSKRRVALFLEQVLNQPCSPGWVVKLQDR
                     ATAAITPAYEELAAQLATEPVLGIDESPTKEAQTKSWLWTFVAGSYTFFALRTTRAAT
                     VLHELLTDAFEGVVNCDRAKMYWKTGRPQWCWAHLKRDFQALIDHPDHQVKRLGRDLM
                     RPTRALFRHWTRYRDGTITRAGMLRLMRPIREEIDGLLLRGAFSGNSKLTGMCGPLYD
                     HREWLWTFLDVEAVEPTNNVSERALRPAVIWRKLSFGTQSARGSRFVEAILTVVETCH
                     QQSRNSFEYLTAAVQ"
BASE COUNT          347 a          509 c          528 g          326 t
ORIGIN      
        1 ctctccttgc gaccctcaaa gcactggtgg ggagcggtcg ccgggccgca accacccggt
       61 gctggtgagc gattggccgg gacttccacc atgtaaaggt cgaccgcgaa tcgtaaattc
      121 cctgcaaatc cgcacttgtg atttccaaat tttacaggaa ttccgtcact tagcaagaat
      181 tctgtcacca attctgtcac cgagggccgg gggcttgcgc gctcaggacg cgcctctcat
      241 tcggcgcgtg aaaacccgcc atcggtactc gcggtcgtaa ccgttcacgc cccggcgagg
      301 cggcgaggcc ggggcgtgca aattgatctt cgcgccggga gcaaaatcag cgacaccagt
      361 ggcatgtcgc aggagccgct gatcacggaa gagctgattg cccggcagcc gccggagtct
      421 caggcaatca ttcggttgtt gttggcgaag atcgccgaac ttgaagcgcg tctgaacaag
      481 acgccgcgaa attcttcgct gccgccgagt atcgagcatc cgcatgccaa ggccacgccc
      541 aagaaaagga agagtggcag gaaacgaggc ggccagcctg gtcatcccaa acacgagcgg
      601 agcttgattc cagtcgagca atgtacgcag gttgttcccg ccattccgga aaactgccgc
      661 cgctgcggcg aagtgctggc cggcttggat tcggaaccgt tgcggcacca agtctgggaa
      721 atccccgaaa tcaaaccgct ggtgacggag tatcaactgc accggctgat gtgtcccgct
      781 tgctccacaa gcacctgtgg ccagctcccg cccggcgttc cgcatgggca agctgggccc
      841 aggctgattg ctctgacggg attgctcatg ggatgcttta aacagagtaa gcgacgtgtg
      901 gccctgtttc tggagcaggt gcttaatcaa ccctgctcgc caggttgggt tgtcaagctg
      961 caagatcgtg cgacggctgc aatcacgccg gcttatgagg agctggcggc acaattggcg
     1021 accgaaccgg tgttgggcat cgacgaatcg ccaacgaagg aagcccagac caaatcttgg
     1081 ctgtggacct tcgtcgccgg cagctatacg ttctttgcgc tgcggacgac tcgcgccgct
     1141 acggtgcttc acgaactgct caccgacgcc ttcgagggcg tggtcaactg cgaccgggcg
     1201 aagatgtatt ggaaaaccgg caggccccag tggtgttggg cgcacctgaa gcgggatttt
     1261 caggccctca tcgaccaccc ggatcaccag gtgaaacgtc tgggacgtga cctgatgcgt
     1321 ccgacacgcg cgctgtttcg acattggact cggtaccgcg atgggacgat tacccgcgcg
     1381 ggaatgttgc gtctgatgcg cccgatccgt gaggagatcg acggcttgct gctgcgcgga
     1441 gcgttcagcg gcaactcgaa actgacggga atgtgcggac ctctgtacga ccatcgcgag
     1501 tggttgtgga ccttcttgga tgttgaggcg gtcgagccca ccaacaacgt aagcgagcgc
     1561 gccttgcggc cggcggtgat ctggcgcaag ctctcctttg gaacccaaag cgcccgcggg
     1621 agccgtttcg tggaggcaat cctgactgtc gtcgaaacct gtcatcagca atcgcggaac
     1681 agcttcgaat acctcaccgc cgccgtgcag
//
LOCUS       DASZYN010000098         5846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_454657, whole genome shotgun sequence.
ACCESSION   DASZYN010000098 DASZYN010000000
VERSION     DASZYN010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454657"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VHE33_02595"
     CDS             <1..598
                     /locus_tag="VHE33_02595"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVT96365.1"
                     /translation="RASQRPIHPAHLAQSVWQAVRDRPWQLANGYLGGWPRRLWDIEE
                     DGQYLGRSGGHGLGYGLPASLGAALAAAGSDTLVVDIQADGDLMYTASALWTAAHHDL
                     PLLVVMHNNRTYGKDELHQREMARLRGRSEDVVPIGIRMSQPPVDFAMLAQSQGVEGI
                     GPVDDPQELPDVLAKAAAVVRAERRPLLVDVLVGGGNG"
     gene            768..1916
                     /locus_tag="VHE33_02600"
     CDS             768..1916
                     /locus_tag="VHE33_02600"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVT96366.1"
                     /translation="MNDFDRRQFLRVAGLSAAAIPLGGVLDACSSSGGSKAPSGGGST
                     SPAAAASGSAGAGSAGTGSAAPATGKVGTMKANNGSAPQDTYVNCVAAPVLYGKDFGL
                     NFTDSDFVIFQSGSAAIQTTLGKQTGAVATGGVSLLEAIAQGAPFKVFQAIAFTDNTV
                     IATNKPNIKTLADIKNMNAVVGVDSNTALEEFDAILLAQNTGFIVSDLKKVQDIESSG
                     ERASALAAGQVDVTIIHQTQADQIASSGKPVHVIASFYSANTKILEQGWMAPTEWLDK
                     NLATAAAFCAATIKGSKALSQDQSAFLSACKQFLKAPPSDDALKADWSLISQHAFWPT
                     TTAGIETDRIQNTIDLAVKEGFIEQGILTADKVIDTRPAQMAMQMLGS"
     gene            1966..2853
                     /locus_tag="VHE33_02605"
     CDS             1966..2853
                     /locus_tag="VHE33_02605"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT96367.1"
                     /translation="MQADAFLEVRNVSKSFRHGRQKTGRMLALSDVSFTMAEGTFLSI
                     IGPSGCGKSTLLRLIDGLQQPDSGEILIDGKRVDRPGRDRGLVFQAPNLMPWRTALRN
                     VTFGLEAQGIHGAERTRRARALLELVGLSEFEDFYPSQLSGGMQQRVGLARALAVNPS
                     VLLMDEPFAAVDAQTKLVLQEELERIWLMEEKSVVFITHDVDEALFLSDTVLVMSKSP
                     GKIIDTVHVDFPRPRSFDIRGSVEFAALVRRLLDSLRHRQPTEDSSWAARSISSKEGP
                     HDGESARLSGPGNVGDGSR"
     gene            2795..3619
                     /locus_tag="VHE33_02610"
     CDS             2795..3619
                     /locus_tag="VHE33_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVT96368.1"
                     /translation="MTVSLPDFPGQVMSETVRGSTHLLRQLGRRRWFRRLVVWTLFAA
                     IYEVAAYIVGPFFLPTIQAVIGSTSGLVSDGAFSSLGGALKQMIVGYLVAVVVGVPLG
                     VLIGVSRVADYILGMYVKALFVTSLIAVLPLLITLVGYGFAFRVTVVLLFAIFFIVLN
                     TAAGVRDVDPQLLSMGRSVGASRLRRAVSITLPSSLPFIISGMRVALANSFGGMILAE
                     IWIARDFGQILTGLAYNRDLPKFMELLWVITLLAAFSAAALKAIERRITPWAASRR"
     gene            3635..4402
                     /locus_tag="VHE33_02615"
     CDS             3635..4402
                     /locus_tag="VHE33_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT96369.1"
                     /translation="MNRWGPRIFEFGLRLASIAAFIGVWQWYGSDKSHIATPPPTRVF
                     PALWNAILHQGLLGRTVGMLVEVGVGLGISLVFGIVVGFAIALFPWARDTIDPLVDAA
                     YAMPTIMLIPIIGVYTGLGFRGRVFIVFLYVALVIVISTSTGVREVDENLIETARSYG
                     FNGLSLFAKVLVPAALAHIASGTAIGVSRAVRGAISAEMLLITANLGGFIVNMQARFN
                     NNALVGGILWTLLIGYALYGLALWVERRITPWRVRAE"
     gene            4486..>5846
                     /locus_tag="VHE33_02620"
     CDS             4486..>5846
                     /locus_tag="VHE33_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96370.1"
                     /translation="MANGSQVQTKTLLATPTLLEAARVIVKGPSFNRTKTAGYDIKPG
                     EKVLLLSKTTDDPAVTEALVTAMREVGASCDVIVLDVPDRPVNALDEYRAMMHNIPGI
                     EPDPNFTRWYQKMRWVEELAVAQQYNLLIQGEAGQLPVLEGGVRYEGAPWFHRVTFPA
                     AGFPWPLWDLINKKAWAPIWEKGRGGRVTLTDPEGTALEWTLFPEHWEAEHYAKTGSR
                     RRFQDEYYLGHLYGIPTPPYEQSDTEGVIAGTINHFGRPFPWCQVSIEAGQVTAVEGG
                     GEYGDKWRSLLEETKQIKYPEFPRPGMFWLWECAIGTHPKMARPPAAFTLSGHATMYE
                     RLRSGYIHLGMGTTVGSPSEAWAAEQGLPYGHIHIHLQFATYVLRTVDGEEITVIRNG
                     HLAALDDPEVVELAKQYGDPYELLQEAWIPPVPGISIPGNYDDYAADPAAWIERYELE
                     GGP"
BASE COUNT         1103 a         1787 c         1861 g         1095 t
ORIGIN      
        1 acgggcttcg cagcggccga tccatccggc acacctcgcg cagtccgtgt ggcaggcggt
       61 gcgcgaccgc ccgtggcagc tggcaaacgg ctacctcggg ggctggccgc gccggctgtg
      121 ggacatcgag gaggacggac agtacctggg ccggtctggc ggccacgggc tcggctacgg
      181 gttgccggcc tccctcggcg cggcgttggc cgcggcgggc agtgacaccc tggtggtcga
      241 tattcaagcc gatggcgatc tgatgtacac ggcatcagca ttgtggacgg cggcgcatca
      301 cgatttgccg ttgctcgtgg tcatgcacaa taaccgcacg tacggcaagg atgagctcca
      361 tcagcgcgag atggcccggt tgcgtggccg ttccgaggat gtcgtgccta tcggcattcg
      421 gatgtcgcaa ccgccggtgg actttgccat gcttgcgcag tcgcaaggcg tcgaaggcat
      481 cgggcctgtt gacgatccgc aggaattgcc cgacgtactc gcgaaggcgg cagccgtcgt
      541 ccgtgctgag cgccgaccgc tgctggtcga tgtcttagta ggtggcggaa atggatgagt
      601 actcgaagca cccaacgacc aacggaccga ggtgtgccgt gtccagcaac gattagcgac
      661 cgcaactgac tgtcacatcc tatttgcaga tccgctcaac gcgagcacat agaactcatc
      721 ttttgcgatc catgtagccg gcgaacacgt tggtgaaagg aagcgacatg aacgacttcg
      781 atcgccgtca gtttcttcgc gttgctggcc tgtctgcggc cgcgattccg ctaggcggtg
      841 tgctggacgc ctgcagcagc agtggaggtt ccaaggcacc gtcggggggc ggttcaacct
      901 cgcccgccgc agcggcgtct ggcagcgctg gtgccggaag cgctggtacc ggaagcgccg
      961 ccccggcaac cggcaaggtt ggcacgatga aggccaacaa cgggtcggcc ccgcaagaca
     1021 cctacgtcaa ctgcgttgcc gcgcctgtgc tgtacggcaa ggactttggc ctgaacttca
     1081 ccgacagcga ctttgtgatc ttccagtcag gctccgcggc catccagacg actctgggca
     1141 agcagacagg agcggtcgca accggcggcg tcagcttgtt ggaagcgatc gcgcaaggtg
     1201 cgccgttcaa ggtgttccag gcgatcgcgt tcaccgacaa cacggtcatt gcgacgaaca
     1261 agcccaatat caagacgctg gccgacatca agaacatgaa cgcggtcgtg ggcgtcgaca
     1321 gcaacacggc gcttgaggag tttgacgcga tcctgcttgc ccaaaacacc ggcttcattg
     1381 tctccgacct gaagaaggta caagacatcg agtcgtcggg cgaacgggcc agcgccctcg
     1441 ctgccggcca ggtcgatgtg acgatcatcc accaaacgca ggctgatcag atcgcttcca
     1501 gcggcaaacc cgtccacgtg atcgcaagct tctatagcgc caacacgaag atcctcgaac
     1561 aaggttggat ggcgcccacg gagtggttgg acaagaacct cgcgaccgct gcggcgttct
     1621 gcgcggcaac gatcaagggc agcaaggcac tgagccagga tcaatctgcg ttcctgagcg
     1681 cttgcaagca gttcctgaag gctcctcctt ccgatgacgc gctcaaggcc gactggagcc
     1741 tcatctcgca gcacgcgttc tggcctacga cgaccgctgg catcgagacc gatcgaatcc
     1801 agaacacgat cgacctcgcg gtcaaagaag gattcatcga gcagggcatt ctgacggcgg
     1861 acaaggtcat cgatacgcga cccgcacaga tggccatgca aatgctgggg tcatagcgta
     1921 cccatgacag ccagcaccag cccccagcgg acgttaggcc caaagatgca ggcagatgcc
     1981 tttctcgaag tccgtaacgt cagtaagtcg tttcggcatg gccggcaaaa gaccggccgg
     2041 atgcttgcgc tgagcgatgt ctcgttcacg atggcggaag gaacgttcct ctcaatcatc
     2101 ggaccgagcg gctgcggcaa gtcgacgttg ctgcgcctca tcgatgggct ccagcaaccc
     2161 gacagcggag agatcctgat cgatggcaag cgagtcgacc ggcctggccg agaccgaggc
     2221 ctggtgttcc aggcgcccaa cctgatgccg tggcggacag ccctgcgcaa cgtgacgttc
     2281 ggtctcgagg cgcagggcat ccacggcgct gagcgaacga ggcgcgcgcg agcgctgctc
     2341 gagctcgtgg ggctctcaga gttcgaggac ttctacccca gccagttgtc gggtggaatg
     2401 cagcaacggg tcggcctcgc gcgagcgctc gcagtcaatc cttcggtgct tctgatggat
     2461 gaaccattcg cagcagtcga tgcgcagacc aagctggtgc tccaagagga gctggagcga
     2521 atttggctga tggaggagaa gtccgtcgtc ttcattaccc acgacgtgga cgaggccctc
     2581 ttcctttcgg acaccgtctt ggtcatgagc aagagccccg gcaagatcat cgacacggtg
     2641 cacgtggact tcccccgtcc gcgaagcttc gacattcgag ggtcggtcga gtttgcagcg
     2701 ctggtgaggc ggctcctcga ctcgctgcgt catcgtcaac caaccgaaga ttcaagctgg
     2761 gccgcacggt ctatctcaag caaagaagga cctcatgacg gtgagtctgc ccgactttcc
     2821 gggccaggta atgtcggaga cggttcgagg tagcacgcac ctccttcgac agctcggacg
     2881 gcgaaggtgg ttccggcgtc tcgtcgtgtg gacgctattc gcggccatct atgaggtcgc
     2941 cgcgtacata gtcgggccat tcttcctgcc gacgatacag gctgtcatcg ggagcacatc
     3001 gggtctcgtg tccgacggcg ccttcagcag cctgggcggc gccctgaagc aaatgatcgt
     3061 cggctatctc gtcgccgttg tcgtaggcgt cccgttgggc gttctcatcg gcgtgtcaag
     3121 ggtggccgac tacatccttg gaatgtacgt caaagccctt ttcgtaacgt ctctgattgc
     3181 cgtgctacct cttctgatta cgctggtggg gtacggcttc gccttccgcg taaccgtcgt
     3241 gctgctgttc gcaatcttct tcatagtgct gaacactgca gccggcgtgc gggacgtgga
     3301 tccgcagttg ttgtcgatgg gccgctcggt tggagcatcg cgtcttcggc gggccgtgag
     3361 catcacgctg ccgtcatctc tgccgttcat aatctctggg atgcgcgtgg cgctggcgaa
     3421 cagcttcggc gggatgatcc tggcagagat atggatcgcg cgcgacttcg gccagatcct
     3481 aaccgggctc gcgtacaacc gcgacctacc caaattcatg gaactgcttt gggtcatcac
     3541 ccttctggca gcattctcgg cggctgcgct gaaggcaata gagcgtcgga tcacaccgtg
     3601 ggcagcgagc cggcggtaga agggtggcgc aaccatgaac aggtgggggc ccaggatctt
     3661 cgagttcggc ctgcgtctcg cctcgatcgc agcgttcatc ggcgtgtggc agtggtatgg
     3721 cagcgacaag tcgcacattg cgacgccgcc accgacgcgg gtctttcctg ctctttggaa
     3781 cgcgatcctc caccaaggct tgctgggcag gacggttggc atgctggtcg aggtaggcgt
     3841 cggacttggc atctcgctcg tgttcggcat cgtggtcggc tttgccatcg cgctctttcc
     3901 atgggcgcga gacaccatcg atccgctagt tgacgccgcg tacgcgatgc cgaccatcat
     3961 gctgataccg atcatcggcg tgtacacggg cctaggcttt cgtggccgcg tcttcatcgt
     4021 gttcctgtat gtcgcactag tgattgtgat cagtacctcg accggcgtgc gggaggtcga
     4081 cgagaacctc atcgagactg cgcgctcgta cggcttcaac gggctctcat tgtttgccaa
     4141 ggtgctcgtc cccgccgcgc tcgcgcacat cgcctccggg accgccattg gtgtgtcgcg
     4201 ggcggtccgg ggcgcgatca gcgcggagat gctgttgatc acggcgaatc tgggcggctt
     4261 catcgtgaac atgcaagcca ggttcaacaa caatgcgctg gtgggcggca tcctgtggac
     4321 gctcctcatc ggctatgccc tgtacggact cgcgctctgg gtagagcgcc gcattacgcc
     4381 gtggcgagtt cgcgccgagt gagctctgga tccgcacggt tccgttcggc tgttcttgcg
     4441 agcacggtct cgcatcgggt tggtcctcgc gaagggtgat gaaggatggc aaacggcagt
     4501 caggtacaga cgaagacgct tctcgctacg ccaactctgc tcgaggcagc acgcgtgatc
     4561 gtgaaggggc cctcgttcaa cagaacgaag accgccggct acgacatcaa gccaggcgag
     4621 aaggtgctgt tgctatcgaa gactacggac gaccctgctg ttacagaggc gcttgtgacc
     4681 gcgatgcgag aggtcggggc gagctgcgac gtcattgtcc tcgatgtccc tgaccggccg
     4741 gtgaacgccc tagacgagta ccgagcgatg atgcacaaca tcccaggcat cgagccggat
     4801 ccgaacttca cgcggtggta ccagaagatg cgctgggtcg aggagctagc tgtcgcccag
     4861 caatacaacc tgctgatcca gggcgaggcc gggcagctgc cggtgctcga gggtggcgtg
     4921 cgatacgagg gcgccccgtg gttccatcgc gtcacgttcc cagccgctgg ctttccatgg
     4981 ccactgtggg atctcatcaa caagaaggcg tgggcaccga tctgggagaa ggggcgtggc
     5041 ggccgcgtga cgctgaccga tcccgagggc accgcgctcg agtggacgct cttcccagag
     5101 cactgggagg ccgaacacta cgcgaagact gggagccggc ggcgcttcca ggacgagtac
     5161 tacctgggtc atctttacgg catcccgacg ccgccgtacg aacaaagcga taccgagggt
     5221 gtcatcgccg gcacgatcaa ccacttcggc cggccgttcc cgtggtgcca ggtctctatc
     5281 gaggcgggac aagtcaccgc cgttgagggc gggggagagt atggcgacaa gtggcgcagc
     5341 ctgctagaag aaacgaagca gatcaagtat ccggagttcc cgcggcccgg catgttctgg
     5401 ctctgggagt gcgctatcgg cacccacccg aagatggcgc gaccacctgc cgcgttcacg
     5461 ctgtccggcc acgccacaat gtacgagcga cttcgctcgg gctacatcca cctcggcatg
     5521 ggcacgaccg tcggcagtcc ctccgaggcc tgggcggccg agcaaggcct tccgtacggc
     5581 cacatccaca ttcacctgca gttcgctacc tatgtcctgc ggacggtcga cggggaagag
     5641 atcaccgtta tccgcaacgg tcatctcgcc gccctcgacg acccagaagt cgtggagctg
     5701 gccaagcaat acggagatcc gtacgaacta ctccaagaag catggattcc gcccgtcccc
     5761 ggcatcagca ttcctgggaa ctacgacgac tacgcagccg atccggctgc gtggatcgag
     5821 cgctatgagc ttgaaggcgg ccccga
//
LOCUS       DASZYN010000099         4643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_133098, whole genome shotgun sequence.
ACCESSION   DASZYN010000099 DASZYN010000000
VERSION     DASZYN010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133098"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VHE33_02625"
     CDS             <1..388
                     /locus_tag="VHE33_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96371.1"
                     /translation="KDFGVPHPTLEQTNACAILTLKPLPAYSGEAYALMGESWESSNL
                     YWARLADEMGYAPESLNILIQELSRRMIAKIFATDLEDWPAILRAMEQTGEEFREGGI
                     RIAGASPIPTNTTSSAQALPLSSSTE"
     gene            394..1512
                     /locus_tag="VHE33_02630"
     CDS             394..1512
                     /locus_tag="VHE33_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVT96372.1"
                     /translation="MKWFLISGLCALLAVTLAVVPGTAAQNGSSRIFVNVVLVQLNVA
                     VTDKKGNYVTGLKPENFLVTEDRIPEKISSFEEGGAPGGILEAGTAPAPENGGGSAPV
                     ETNTAGQQQTAPRQAEASALQLGLPKASHLAGASVFVLFDTSDYMYRGFVFAQDAIGG
                     FVRTMEEANKIAFYSYSRNLSRATLLTTDRSVVMRGVRNTVAGDEAALYNALLLTVRD
                     AAQLHGRKAIVVFSNGPDNASTVPPEDVEELAQSTGTIIYIISTRQARDEPISSAVFE
                     RMSRGTGGKAYFAKDWRDESMAFASIKEDLAHLYTISYYPKLNQNRGWRTISVRLVGP
                     GMEKYRVRTRDGYRLQEQVPQPSAQAAAEPPATPTNSN"
     gene            complement(1532..2248)
                     /locus_tag="VHE33_02635"
     CDS             complement(1532..2248)
                     /locus_tag="VHE33_02635"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HVT96373.1"
                     /translation="MPTRSDEPAYRKIQNAIRRRIDSGQLKPGDVVPSERELARIHSV
                     SLMTARHALTELARDGIVERRHGAGTFVAPPKVQFNKLLGYSEQMASLGLTARSRIVS
                     SSVVTREHEIAARLGLPPTARLGRLERVRLAGDETAALEICYWSADEYPALQHAPLDR
                     VSLFSVLEHDCGVQLAYADEEIDATDADQRMADLLSLSRGTPLLRIRQLIFSNTGRAT
                     VYVTGFYRSGRHTLRIRRFR"
     gene            2455..3948
                     /locus_tag="VHE33_02640"
     CDS             2455..3948
                     /locus_tag="VHE33_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96374.1"
                     /translation="MKKSIVILALAFVVWMPGLRAQETIAVDAQAATTSFPHFWEEMF
                     GSGHAILALREDYREDLRAVKGVTDFRYVRFHGILDDEVGSYKEDEHGEPVYNFAYVD
                     AIYDGLLKNGVRPVVEISFMPTKLAFNPLDLHVFWYHPNVSPPKSWERWDAYMTAFAQ
                     NLVNRYGIDEVAQWYFEVWNEPNIDFWGGIPRQGSYFELYAHTARDLKKVSPRLRVGG
                     PATAAASWVPEFLSYVTKNHVPIDFVSTHGYADDTVENLFHSNEDIPEDDRVCRAVEK
                     VQGEIRASGKPDLPLFWTEWNVEGRDESRDTTFVGPGVANTIRACDGHVTMMSFWTFD
                     DVFEEGGPIPKPFEGHFGLIAKGGIRKPSFYDFALLHQLGDRRIALRASDAIATKAED
                     GSLRVAVWNIADPGTHGSSRAVELTFSGVAADATVGVERVDQDHSNVLPKYAAMGSPV
                     DPTPEQVEELNRETALGPAEQTHLEGGKLKLTLTPNALVLLKVDAGH"
     gene            4012..>4643
                     /locus_tag="VHE33_02645"
     CDS             4012..>4643
                     /locus_tag="VHE33_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96375.1"
                     /translation="MNVIEWLLDSDPAIRWQAMRDLTDAPAATIVAERARVSQEGVGA
                     AILAGQEPDGAWRRADAPTWLTTLFTMQLLRATGADRTDPIVVSAVARAEVHLQWNVH
                     NDCWDLRSPEFGGNAFFLGEEEPCINGGVLALGGYFGRPNENLARRLLNEQMEDGGWN
                     CEAPKSRRSSFHTTICVLEGMLEYERAVGGAPEIAEARRRGEEYLLARGL"
BASE COUNT          858 a         1209 c         1684 g          892 t
ORIGIN      
        1 gaaggatttc ggggtgccgc atccgacttt ggagcaaacg aacgcgtgcg ccattctcac
       61 gctcaagccg ctgcccgctt attccggcga agcgtatgcg ttgatgggcg aatcgtggga
      121 atcgagcaat ctctactggg cacgcctggc ggatgagatg ggctacgcac cggagtcgct
      181 gaacattctg attcaggaac tgagccggcg catgattgcg aaaattttcg ccaccgacct
      241 tgaggactgg ccggcgattc tgcgagccat ggagcagacg ggcgaggagt tccgcgaggg
      301 aggcatccgg attgcgggtg cgagtccgat tccgaccaac accacgagca gcgcgcaggc
      361 gctgcccctg agttcttcca cggagtagtg acgatgaaat ggttcctgat ttccggtttg
      421 tgtgcgcttc ttgcggtaac gctcgcggtc gttccgggca cggcggccca gaacggctcg
      481 tcgcgcattt tcgtcaacgt cgtgctggtg cagctgaacg tggctgtgac cgataagaag
      541 ggcaactacg tcacaggcct gaagccggag aatttcctcg tgactgagga caggattccg
      601 gagaagattt cctcgtttga ggagggcggc gcgccgggcg ggatcctgga ggcggggacg
      661 gcccctgctc ctgagaatgg cggcggcagt gctccggtgg agacgaacac ggcggggcag
      721 caacagaccg ctcccaggca ggctgaggct tcggccttac agctggggct gcccaaagcc
      781 tcgcatcttg cgggcgccag cgtgtttgtt ctgttcgaca ccagcgacta tatgtatcgc
      841 ggctttgttt ttgcgcagga tgcgatcgga ggcttcgtgc gcaccatgga agaggccaac
      901 aagatcgcgt tctattctta cagccggaat ttgtcgcgag cgacgctgct gacgaccgac
      961 cgatccgtgg tcatgcgggg ggtgcgcaac acggtagccg gcgacgaggc ggcgctgtac
     1021 aacgcgctgc tgctgacggt gcgtgatgcg gcgcagctgc acggacgcaa agcgattgtg
     1081 gtcttctcca atgggccgga caacgcgagc acggtgccac cggaggacgt ggaggaactg
     1141 gcgcagtcga cgggcacgat catctacatc atcagcacgc ggcaggcgcg cgatgaaccg
     1201 atctcgagcg cggtgttcga gcgcatgagc cgagggacgg gcggcaaggc ttacttcgcc
     1261 aaagactggc gcgacgagag catggccttc gcttcgatca aggaagatct cgcgcacctc
     1321 tacaccatca gctactaccc gaagctgaat cagaatcggg gctggcggac gatttcggta
     1381 cggctggtgg gaccgggcat ggagaaatat cgcgttcgca cgcgcgacgg ttatcggctg
     1441 caggagcagg tgccgcagcc ttcggcgcag gcagcagcgg aacctccggc aacgccgacg
     1501 aattcgaact gacgcgctgc gccggccgct gttagcggaa gcggcggatg cgcagcgtgt
     1561 ggcggccgga tcggtaaaag ccggtgacgt agacggtagc gcgtccggtg ttggagaaga
     1621 tgagctggcg aatgcgcagc aggggcgtgc ctctggagag agagaggaga tcggccatgc
     1681 gctgatcggc gtcggtggcg tcgatttcct catcggcgta ggccagctgg acgccgcaat
     1741 cgtgctcgag aacggagaac agcgagaccc tgtcgagcgg ggcgtgctgc agggccggat
     1801 actcgtcggc cgaccaatag cagatttcca gggcggcggt ttcatcgccc gccaggcgca
     1861 cacgctcgag ccttcccagg cgcgcggttg ggggcaggcc cagacgcgcg gcaatctcgt
     1921 gctcccgggt aacgaccgac gaggacacga tgcgggagcg cgcggtgagc ccgaggctgg
     1981 ccatctgctc gctgtaaccg agtagtttat tgaactggac cttgggggga gcgacgaagg
     2041 ttcccgcgcc gtgacgccgc tccacgatgc cgtcgcgggc cagctcagtg agcgcgtgac
     2101 gtgcggtcat caggctcacg gaatgaatcc ttgccaactc gcgctcggaa gggacgacgt
     2161 cgccgggctt cagctggccg gagtcgatgc ggcggcggat cgcgttctga atcttccggt
     2221 aagctggttc gtcgctgcgg gttggcatgg tcgcgaaatt tccgttacgg ccgaaatgct
     2281 cagccaggtc agcaggttgg ggtaacgttt tccctgaaca cgatatcaga accaggtaag
     2341 actcatggac ctgcctgcga ttcttcgggg cgccctgcat tgtgctatag agcgccccat
     2401 cgcatgggca gggtgacgac aggccgcggg gcatccaacg tggcggaggt acgcgtgaag
     2461 aagtcgattg tcattttggc gctggcgttt gtcgtatgga tgccgggttt gcgtgcgcag
     2521 gagacgattg ccgtcgatgc acaggcagcg acgacgtcgt ttccgcattt ttgggaggaa
     2581 atgttcggct cggggcacgc catcctggcg ttgcgcgagg actatcgcga ggatctgcgc
     2641 gcggtgaagg gcgtgacgga tttccggtac gtgcgatttc acggaattct cgacgatgag
     2701 gttggcagtt acaaggaaga tgagcatggt gagccggtct acaacttcgc ttacgtcgat
     2761 gcgatttacg acgggttgct gaagaacggg gtgcggccgg tggtggagat cagctttatg
     2821 ccgaccaagc tggcgttcaa tccgctggac ctgcatgtgt tctggtacca cccgaatgtg
     2881 tcgccgccga agagttggga gcggtgggac gcgtacatga cggcgttcgc gcagaacctg
     2941 gtgaatcggt acggcatcga tgaagtggcg caatggtatt tcgaggtgtg gaatgagccg
     3001 aacatcgatt tctggggagg aattccgcgg cagggcagct acttcgaatt gtacgcgcat
     3061 acggcgcggg atctgaagaa ggtcagccca cgcctgcgcg tgggtggtcc ggcaacagcg
     3121 gcggcatcgt gggtgccgga gtttttgagt tatgtgacga agaatcatgt gcccatcgat
     3181 tttgtgtcga cgcacggata tgccgatgac acggtggaga atctgttcca cagcaacgaa
     3241 gacattccgg aggacgatcg ggtttgccgt gccgtggaga aggtgcaggg ggagattcgc
     3301 gcgtcgggca agccggattt gccgctgttc tggacggagt ggaacgtgga gggacgggac
     3361 gagtcgcgtg acacgacgtt tgttggccct ggagtcgcga atacgattcg cgcatgcgac
     3421 gggcacgtga cgatgatgtc gttctggacc ttcgacgatg tattcgagga aggcggtccg
     3481 attccaaaac cgtttgaagg gcatttcggg ctgatcgcga agggcggcat tcgcaagccg
     3541 agcttctatg atttcgcgct gctgcatcag ttgggggatc ggcggattgc gctgcgggca
     3601 tccgacgcca ttgcgacgaa ggcagaggat ggttcgctgc gcgtggcggt gtggaatatt
     3661 gcggatccgg gaacgcacgg gtcgtcacgg gcggtcgagc tgactttttc cggtgttgcg
     3721 gcggatgcga ctgtgggtgt tgagcgggtc gatcaggacc atagcaacgt gctgccgaag
     3781 tatgcggcga tgggttcgcc ggtggatccg acgccggagc aggtggagga attgaatcgg
     3841 gaaacggcgc tggggcccgc ggagcagacg catcttgagg gtggcaagct gaagctgacg
     3901 ctgacgccga atgccctggt gctgttgaag gtggacgccg ggcattagtt gcgtccccat
     3961 gagcgagggt cgctcgcggg atcgcagtgc cggctttggt aagatcgcgc cgtgaatgtg
     4021 atcgagtggc tgcttgattc ggatccggcg atccggtggc aggcgatgcg tgatctgaca
     4081 gatgcgccgg cggcgacgat tgttgcggag cgcgcgcgtg tttcgcaaga gggcgttggc
     4141 gcggcgattc ttgccggaca ggaaccggac ggcgcgtggc ggcgggctga tgctcccacg
     4201 tggctgacga cgctgtttac gatgcagctg ctgcgcgcaa ccggagcgga ccgcacggat
     4261 ccgatcgttg tgtcggcggt ggcgcgggcc gaggtccatc tgcagtggaa cgttcataac
     4321 gattgctggg atttgcgctc gccggaattt ggcgggaatg cgttctttct gggcgaggaa
     4381 gagccgtgca ttaacggcgg tgtgctcgca ctgggcgggt atttcgggcg gcccaatgag
     4441 aatctcgcgc gccgattgct gaacgagcag atggaagacg gaggctggaa ctgcgaggct
     4501 ccgaagagcc ggcgctcgtc gtttcacaca acgatttgcg tgctggaggg gatgctcgaa
     4561 tacgagcgag cggtgggagg tgcgccggag attgcggagg cgcgacggcg gggtgaggag
     4621 tatctgctgg cgcggggttt gtt
//
LOCUS       DASZYN010000100         4991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_133129, whole genome shotgun sequence.
ACCESSION   DASZYN010000100 DASZYN010000000
VERSION     DASZYN010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133129"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VHE33_02650"
     CDS             <1..333
                     /locus_tag="VHE33_02650"
                     /inference="COORDINATES: protein motif:HMM:NF025465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6807 family protein"
                     /protein_id="HVT96376.1"
                     /translation="EGVKGDAVWSTRGRWCSLTGHDPEHHTVTIAILDHPANPGYPTY
                     WHARGYGLFAANPLGRSIFDPKQPAFNYTLNQGQTATFRYRVLIISNEATPESLNREA
                     DHFAVAYR"
     gene            complement(422..1471)
                     /locus_tag="VHE33_02655"
     CDS             complement(422..1471)
                     /locus_tag="VHE33_02655"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HVT96377.1"
                     /translation="MGPVASPQRTLDEAGKHGVAFQYQYTHDLSAIPLGNSMEHGWFG
                     RSTWTLSAAVDLEKTMGWRGTSGVVSMKQHVLEFGRMDRGFAQGYSNLDADPYTMLYE
                     GWIQQQTLGGKLTLKAGQMDSNADFDALPLAGDFLNSSMGYSPTVMEFPSYPVPQPAV
                     EALANLGRATRVAAGEFRTAGGRMSLFQGDRTWAIGTGERAGRAAVGMWHLTESLARP
                     DGVAVRGTNGFYGVLEQSLWNGPLADGKTRTLSGYLQAGTGAARVNPFRAHLGGGVVM
                     SGQWGKRPSDAVGAAASWVDLGSAGRETVVETYYKLCLGKAVSLVPDVQYFRNPGGSP
                     HGEALVVTPRLVFAF"
     gene            1900..2424
                     /locus_tag="VHE33_02660"
     CDS             1900..2424
                     /locus_tag="VHE33_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96378.1"
                     /translation="MRTAVKIVLIVLAAVLVPIAASAETCALQTYASSWMATGTPFSV
                     KCPSGTYSGHLVSTPARRFFRRGHLMLVFDQPVVLVPKKPGDEGKIQAGRGRQIANML
                     MTGGVSIGSKDLSDGISGAVFKSWYMIPISAVAISFFSTGGDVLLKPGYKLQVESRTS
                     HNGNESGQAMPSGR"
     gene            complement(2445..4886)
                     /locus_tag="VHE33_02665"
     CDS             complement(2445..4886)
                     /locus_tag="VHE33_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT96379.1"
                     /translation="MNVWLQDLRYAVRMLRRNTVLSVVILASLGIGIGANSAIFSVVD
                     ALLLRPLPYPHADRLASVWLHSPGIGILRDWPSPGQYVDLQHENHSFEAMALAQSRAF
                     TLTGRDQPERVEILKTQSSLFEMLGARTLLGRTLLPEEDKPGQPAVAILSYGVWQRLF
                     SSDPAIVGKSIQLNGQPYLVAGVLRRQFTVNDEVLPSEGPMDKIDIYVPLPLGADAQQ
                     RRGDENYNILVRLKPGVSVQQAQADIDVIASRIREKDKRGATFGMDVVGLQQQVVGDV
                     RRALLVLLGSVALVLLIACANVANLLLARAAGREKEVAIRTALGANWKRLTRQLLTES
                     ILLGLLGGVAGLVVARLSLWVVRTMNPGNIPRLDEIGINGTVLAFTFGLSLATGVLFG
                     LAPIWRVLRLDANASLKAGGRSGQGDGGLYLKRHRLRGLLVVSEIALSLILLVGAGLL
                     IRSFVRLQKVPPGFTTDHVVTMEAVVSDPKYKDDKILDGFYREAEGRIAHLPGVVAEG
                     AVSALPLTGSVGWGSIHVEGYAPPPGQELQVDLRTATTDYFRAMEIPLVAGRFFTEQD
                     NPDAPQVAVIDAKFAQRFWPHGDAIGKHLWFDPKKPIMIVGVVGVVKQYGLETDGKIA
                     TYFPMLQHPDRGTFLVARTETDQAGLSSAMIQEIHAVDPNAVVYGVRTMQDRLFDSLA
                     RQRFATTMLGAFAAFALLLAAVGLYGVMSYLVTQSTRDIGILVALGAGRDTILGLVVK
                     QGMLLAMIGIVAGLAGAVALTRVIASLLFGVSATDVATFMVVPGLLAVVAFAATVIPA
                     WRASSVDPMVALREE"
BASE COUNT         1013 a         1833 c         1248 g          897 t
ORIGIN      
        1 gaaggcgtca aaggcgacgc cgtctggagc acgcgcggcc gctggtgctc cctcaccggc
       61 cacgaccccg agcaccacac cgtcaccatc gccatcctcg accaccccgc gaatcccggc
      121 taccccactt actggcacgc gcgcggctat ggcttattcg ccgccaatcc gctcggccgc
      181 agcatcttcg atcccaaaca gcccgccttc aactacaccc tcaatcaagg acagaccgcc
      241 accttccgct atcgcgtgtt gatcatctca aacgaagcca cgccggaatc actgaaccgc
      301 gaagccgacc acttcgccgt tgcctatcgc taacgaaaga tcggcaagag gctctcttca
      361 aacggacaaa agggtagcgc aagctaccct tctccttttc ctttgagtcg atgaccactc
      421 atcagaacgc aaacaccagg cgcggagtca ccaccagcgc ctcgccatgc ggactcccac
      481 ccggattccg gaagtactgc acgtccggta ccagcgaaac cgccttgccc aggcatagct
      541 tgtaatacgt ctccaccacc gtctcccgtc cagccgatcc caggtccacc cagctcgccg
      601 ctgctccaac cgcgtccgat ggccgttttc cccactggcc cgacatcacc accccgccgc
      661 ccagatgcgc ccgaaaagga ttcacccgcg cggctcctgt tcccgcctgc agatatccag
      721 acagcgtccg cgtctttcca tcagccagcg ggccgttcca cagcgattgc tccagcactc
      781 catagaatcc gtttgttccc ctgacggcta ctccatccgg tcgcgccagc gattcggtca
      841 gatgccacat cccgaccgcc gcacgccccg ctcgttcacc ggtgccaatc gcccaggttc
      901 gatcgccttg aaacaaactc atccgcccac ctgctgtccg gaactctccg gctgccaccc
      961 gtgtcgctcg tcccaggttc gccaatgcct ccaccgcagg ttgcggtacc ggatagctcg
     1021 gaaactccat caccgtcgga ctgtagccca tcgaagagtt cagaaaatca cccgccagcg
     1081 gcagcgcgtc aaagtccgca ttcgaatcca tctgcccggc cttcagcgtc agcttccctc
     1141 ccagcgtctg ttgctggatc catccctcgt acagcattgt gtagggatcc gcatccaggt
     1201 tcgaataccc ctgcgcgaaa ccgcgatcca tccgcccaaa ctccagcaca tgctgcttca
     1261 tgctcacaac gccgctggtc cctcgccatc ccatcgtctt ttccagatcg accgcggcgg
     1321 aaagcgtcca cgtcgaccgc ccaaaccagc catgctccat ggaattcccg agcggaatcg
     1381 cagaaagatc gtgcgtgtat tgatactgaa aagcgactcc gtgcttcccc gcctcgtcca
     1441 gcgtccgttg cggactggca accggcccca accaccgctc tgcccaacca tgtgcatcgt
     1501 cctgctggcc ccaacctgcc ggcgccaaca gacaaaagag aacgcctgtt accgctccgc
     1561 gccatctatt tccgagatcc cttataccgc gatccgaata tccgacaaca accgtcctat
     1621 ttaaagttcg actgtttcgg tccgcggtaa tcgcggtcac accgttgctt cttccccatt
     1681 tgccctgcac cgatcacctg ccgatgagaa atctctaatg acttccccat cggcggcctg
     1741 acgccgggcg gcagagtgtc acaaaacaat acgcagaagc tgctgcatcc tctgtcaggt
     1801 cctggatcgc accaaccacg ccgagtcttt cctcttcatg gatccccaac agccggcccg
     1861 tataatcgac ccgcccgacc tacagcaacg aagagattcg tgagaaccgc cgtgaaaatc
     1921 gtcctgatcg tcctcgcagc cgtcctcgtc cccatcgcag cgagcgctga aacctgcgcc
     1981 ctccagacct acgcatcgtc ctggatggcg accggcacgc ccttctcggt caagtgccca
     2041 tccggaacct attccgggca cctcgtttcc acgccggcgc gccgcttctt ccgtcgcggc
     2101 cacctcatgc tcgtctttga ccagccggtg gtcctcgttc cgaagaagcc cggcgatgaa
     2161 ggaaagattc aggccggccg aggccgacag atcgccaata tgctcatgac cgggggagtc
     2221 tcgatcggat ccaaggacct cagcgacggc atctccggag ccgtcttcaa atcctggtac
     2281 atgatcccca tctcggctgt tgccatttcg ttcttctcca ccggaggcga tgtcctgctc
     2341 aaacccggat acaaactcca ggtcgaatcg aggacctcac acaacggcaa tgaatcgggg
     2401 caggcgatgc cttcggggcg ttaagcagaa ctcagtgcga atcgctattc ttcgcgaagc
     2461 gccaccatcg gatccacact actcgcgcgc cacgccggaa tgaccgtcgc cgcgaaggcc
     2521 acgaccgcca gcagcccagg cacgaccatg aacgtcgcca cgtcggtcgc gctgactccg
     2581 aacagcaggc tggcgatcac ccgcgtcagc gccaccgcac ccgccaagcc ggcaacgatg
     2641 ccgatcatgg ccagcagcat gccctgcttc accactaaac cgagaatcgt gtcccgcccc
     2701 gctcccaacg ccaccagaat cccgatgtcc cgcgtactct gcgtcaccag ataggacatc
     2761 actccgtaaa ggcccaccgc cgccagtaac agcgcaaacg cggcgaaggc tcccagcatc
     2821 gttgttgcga accgctgccg cgccagcgaa tcgaacaacc ggtcctgcat cgttcgcacg
     2881 ccgtaaacca cagcgttggg atcgaccgca tggatttcct ggatcatggc gctggagagc
     2941 cctgcttgat ccgtctcggt acgagccacc aggaatgtcc cacgatccgg gtgctggagc
     3001 atcgggaaat acgttgcaat cttcccgtcg gtctcgagac catattgctt gaccacaccc
     3061 acgacaccga cgatcatgat tggcttcttc ggatcgaacc acagatgttt tccgatcgcg
     3121 tccccatggg gccagaatcg ctgcgcgaat tttgcgtcaa tgacggccac ctgcggcgcg
     3181 tccggattgt cctgctccgt gaaaaaccgc cccgctacca aaggaatctc catcgcccga
     3241 aaatagtccg tggtcgctgt gcgaagatcc acctgcagct cctgccccgg cggcggcgca
     3301 tatccttcga cgtggatgct tccccagccc accgatccgg tcagcggcag cgccgacacc
     3361 gcaccttcag ccaccactcc aggcaagtgc gcgatccgcc cttccgcctc acggtagaag
     3421 ccgtccagaa ttttgtcgtc cttatacttc gggtcgctca ccaccgcctc catcgtgacc
     3481 acgtggtcgg tggtgaatcc tggcggtact ttctgcaaac ggacgaagct tcgaatcagg
     3541 agcccagcgc ccaccagcag aatcagcgaa agagcaattt cagaaaccac cagcaatccg
     3601 cgcagacggt ggcgcttcag gtagagtccg ccgtcgccct gcccgctccg tccgcctgcc
     3661 ttcagcgacg catttgcatc cagcctcagc actcgccata tcggcgcaag tccaaagagc
     3721 acgccggtcg cgagtgaaag cccaaacgta aacgccagca ccgttccgtt gatgccaatc
     3781 tcatccagtc gcggaatgtt ccccggattc atcgtgcgca cgacccacag gctcagccgc
     3841 gccaccacga ggccggcaac tcctcccagc agtccgagca gaatgctctc cgtcagcaat
     3901 tgccgcgtca gccgcttcca gttggcgccc agtgccgtgc ggatcgccac ttccttctcg
     3961 cgccccgcgg cccgcgccag cagaaggttc gcgacattgg cgcacgcaat cagcagcacc
     4021 agggccactg agcccagcag caccagcagc gctcgccgca cgtctcccac cacctgctgc
     4081 tgcagcccca ccacatccat gccgaaggtc gcaccgcgct tgtccttctc gcggatgcgg
     4141 ctcgcgatca cgtcgatgtc cgcttgtgcc tgctggacag aaacgccagg ttttaaccga
     4201 accaggatgt tgtagttctc atcgccgcga cgctgttgcg catcggcccc cagaggcagc
     4261 ggaacataaa tgtcgatctt gtccatcggc ccttccgacg gcaacacttc gtcgttgacc
     4321 gtaaactggc gtcgcaacac gcctgccacc agatagggct gtccattgag ttgaatgctc
     4381 ttgccgacga tggctgggtc ggaactgaac agccgctgcc aaacgccgta gctcagaatc
     4441 gccaccgccg gctggccggg tttgtcttcc tccggcaaca gcgtacggcc cagcagcgtc
     4501 ctcgccccca gcatttcgaa caggctcgac tgcgtcttca ggatctccac gcgctccggc
     4561 tgatcgcgac ctgtcagcgt gaaggctcgg ctttgcgcca gcgccatcgc ctcgaacgaa
     4621 tggttctcgt gctgcaagtc gacgtactgt cccggcgacg gccagtcccg cagaatgcca
     4681 atccccggcg aatgcagcca caccgacgcg agccggtctg catgcggata aggaagtggg
     4741 cgcagcagca gcgcatcgac cacgctgaaa atcgccgaat tcgcgccgat cccgatcccc
     4801 agcgaggcca gaatcaccac cgacagcacc gtgttcctgc gcagcatgcg cactgcgtag
     4861 cgaagatcct gcagccagac gttcatcgac cctcccgcgc tgataacgag gatcatagga
     4921 cacggagaca ctctctggct gccgtgaaac aggtttgtga cggcttggct acggctctcc
     4981 ggggtgcccc a
//
LOCUS       DASZYN010000101         1593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_966222, whole genome shotgun sequence.
ACCESSION   DASZYN010000101 DASZYN010000000
VERSION     DASZYN010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966222"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VHE33_02670"
     CDS             <1..348
                     /locus_tag="VHE33_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HVT96380.1"
                     /translation="NQLDGLVYNIEKMLKENGDKVSGSDKSDVETALAEAKKTLEGSP
                     SVADMNAAREKLTQASHKLAEAMYKANAANPGAPGAGVPPTDGDAAAAGTADGKKDEG
                     VIDAEYVDVEDKK"
     gene            677..847
                     /locus_tag="VHE33_02675"
     CDS             677..847
                     /locus_tag="VHE33_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96381.1"
                     /translation="MTNTLWKCEQLRAGQITNQVVFDSENQARHFVREMGKVEPDIFW
                     RIEPVPAMVVWN"
     gene            1180..1500
                     /locus_tag="VHE33_02680"
     CDS             1180..1500
                     /locus_tag="VHE33_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HVT96382.1"
                     /translation="MPPLHPGEMLREEFMKPLGLSANALALALRVPVTRITEILNERR
                     SITADTALRLSRYFRMSANFWLRLQAQYDLEVVEDEIASVIRREVRPAPRDAKTGELK
                     PATA"
BASE COUNT          374 a          430 c          512 g          277 t
ORIGIN      
        1 aaccagctcg acggcctggt ctacaacatc gagaagatgc tgaaggagaa cggcgacaag
       61 gtgtccggct ccgacaagtc cgatgtggag acggcgctgg ccgaggcgaa gaagacgctg
      121 gaaggcagcc ccagtgttgc cgatatgaat gcagcgcggg agaagctgac ccaggcgtcg
      181 cacaagctgg ccgaggccat gtacaaggcc aacgcggcga atccgggcgc gccgggcgcg
      241 ggggttcctc caaccgacgg cgacgcggcc gcggccggca ccgcggacgg caagaaggac
      301 gaaggagtca tcgacgccga gtacgtggac gtcgaggaca agaaataacc gggctcagat
      361 cacagggctc gggtcgcaga actaaggagg ggcgttcttt ggaacgcccc gcagcctggc
      421 atgacggtaa aagccccggc ctccaggccg gaacctggcc aggaaggtaa cccgggcttc
      481 ggcgggagcc tttgtatcgg ggcacgactc aaagggtgcc ccaccatctg cccggttttg
      541 gcagatgtgg gaacgcgcga tcttagtcag ccgcttttgt atcagggcat gacttcagtc
      601 gtgccgacag ggaagcaaat caaaacgggc tttagcccct gaggagcggc agttgcagtt
      661 gaggagttca atcgggatga cgaacacact ttggaaatgc gaacagttgc gcgccgggca
      721 gatcacgaac caggttgtgt tcgacagcga gaatcaggcc cggcatttcg tgcgcgagat
      781 ggggaaggtg gagccggata ttttctggcg tattgagccg gtaccggcca tggtggtttg
      841 gaactagcgt ttaacgcccg gcgttaaacg tgctacgcta aagcgtgatc cagtctttcg
      901 ggaacaagga aaccgagcgg atctacaggc gtttacatag ccgccagttt gcttcgattg
      961 aacggattgc tctccggaaa ctcaggcaaa ttgcgaacgt ggatcgcacc gaagagctgg
     1021 cagagccgcc cgggaacaga ctggaaaagc tgcgtggaaa tcgtcaggat cggtggagta
     1081 tccggatcaa cgaccaatgg cgtgtctgct ttgaatggag ggatggagat gcctggcagg
     1141 tcgaaatcgt cgactaccat taagcccgga ccacagaaga tgccgcctct gcatccaggc
     1201 gagatgctgc gcgaagaatt catgaagcct cttgggctgt cggctaatgc actcgccctg
     1261 gctttgcgtg tccccgtaac gcgcatcact gaaattctca acgagcgtcg cagcatcacg
     1321 gcggacaccg cgctgcgtct gtcgcgctat ttcaggatgt cggccaactt ctggttgcgt
     1381 ctgcaggcac agtacgacct ggaagttgtc gaggatgaga ttgcgtctgt tatccgccgc
     1441 gaggttcgtc ctgcgccgcg cgatgccaaa accggagaac tgaagccggc aacggcctga
     1501 atcgaaaacg agaagaatgc caccaactca gcacaaagac tattacgcga ccctaggggt
     1561 gaagaagtct gccacctccg aggacattcg caa
//
LOCUS       DASZYN010000102         3689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1225528, whole genome shotgun sequence.
ACCESSION   DASZYN010000102 DASZYN010000000
VERSION     DASZYN010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225528"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2733
                     /locus_tag="VHE33_02685"
     CDS             <1..2733
                     /locus_tag="VHE33_02685"
                     /inference="COORDINATES: protein motif:HMM:NF016859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96383.1"
                     /translation="SHNILSPASGQPITVPTQDMVLGLYYLTKAKKGAKGEGRAFANI
                     EEVLMALNAHEVETLSPIRLRYTGQVLEMSTAYDDQDLTHTEPVQYDRQFISTTVGRA
                     ILNDALPQEMPYVNGLLKKKGIGQLVNYCYLNHGLELTVQMLDRIKELGFQYATRSGL
                     SVGLDDMVIPEAKYATVRDAEKQAIAVQQQYLDGAITNGERNNKVIQIWSAVTERVAD
                     EMFDNMKRADKEGAMNPIYIMADSGARGSKQQIRQLSGMRGLMAKPSGEIIETPITAN
                     FREGLTVLEYFISTHGARKGLADTALKTADSGYLTRRLVDVAQDVIVSERDCKTVEGI
                     YVTPIVESGEIIEPLRDRIVGRVSLEKIKDYEGQVIVDINQEITEDLASAIQAAGIER
                     VKIRSVLTCESRRGVCILCYGRNLGSGRLVELGEAVGVIAAQSIGEPGTQLTMRTFHI
                     GGTASRVNEQSRLEAKNNGSVRFINLATVRSRQGDLVAMNRSGSIAVVDERGREKERY
                     QVVYGAHIKIEENQPVQLGQVMVEWDPYTFAILTEIGGTVGFKDLQEGITLHEEVDEV
                     TGLSRLVVGDSPDEKRQPAIVIKGGKGQKRYLMPSRSHLMIQDGDEVSPGDILAKIPR
                     ETTRTKDITGGLPRVVELFEARKPRETAIISEIDGVVRFGEVSKGQRRIYVTADNGEE
                     KEYSVPRGVHINVQEGERVRAGEPLMDGPLNPHDILAVLGEKELQAYLVNEIQEVYRL
                     QGVAISDKHIETIVRQMLRWVKIEEVGDSSFLLEQQVDKFRFREETQRVITNGGRPPV
                     GRPLLLGITKASLSTESFISAASFQETTRVLTEASINGAVDNLRGLKENVIVGRLIPA
                     GTGMEYYRNVQLSPELEEAAAKIQQEVQTAWEEAERELEMMRQEGEAEEMAASE"
     gene            complement(3188..>3689)
                     /locus_tag="VHE33_02690"
     CDS             complement(3188..>3689)
                     /locus_tag="VHE33_02690"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HVT96384.1"
                     /translation="WNFDLAHECGHIAMHRDTPTGALETERAADRFASAFLLPANAFG
                     REFRTKPFTWQHVFDLKQRWQVSIAAIVRRARDLGLLDEVAYRRAFQYMSSKNWRREE
                     PFEPHFQEPELFVQAIDALGGHVKKTLPELCADLGFSGPVFTEIAGISLKAVSQKRAG
                     FLTLVK"
BASE COUNT          804 a         1225 c         1035 g          625 t
ORIGIN      
        1 tcgcacaaca tcctctcgcc cgcgtcgggt cagcccatca ccgtgcccac ccaggacatg
       61 gtgctcggcc tctactatct gaccaaggcg aaaaagggtg cgaaggggga aggccgcgcc
      121 ttcgccaaca tcgaagaagt cctcatggct ctcaacgctc atgaggtcga gaccctcagc
      181 ccgatccgtc tccgctacac cggccaggtg ctcgaaatga gcaccgccta cgacgatcag
      241 gacctcaccc acaccgagcc ggtccagtac gaccgtcagt tcatctccac cacggtcggc
      301 cgcgccatcc tcaacgatgc gctgccccag gagatgccct acgtcaacgg cctgctcaag
      361 aagaagggca tcggccagct cgtcaactac tgctacctca accatggcct cgagctcacc
      421 gtgcagatgc tcgaccgcat caaggaactc ggcttccagt acgccacccg cagcggcctc
      481 tccgtcgggc tcgatgacat ggtcatcccc gaagcgaagt acgccacggt tcgcgatgcg
      541 gaaaagcagg ccatcgccgt ccagcagcag tatcttgacg gcgccatcac caatggcgag
      601 cgcaacaata aggtcatcca gatctggtcg gccgtcaccg aacgcgtcgc cgacgagatg
      661 ttcgacaaca tgaagcgcgc cgacaaagag ggagccatga acccgattta catcatggcc
      721 gactccggcg ctcgcggttc caaacagcag atccgccagc tctccggcat gcgcggcctc
      781 atggccaagc cttccggcga aatcatcgag acccccatca cggctaactt ccgtgaaggc
      841 ctcaccgtgc tggaatactt catctccacg cacggcgcgc gcaagggcct cgccgatacc
      901 gcgctcaaaa ccgctgactc cggctacctc acccgtcgtc tggtcgacgt ggcccaggac
      961 gtcatcgtct ccgagcgcga ctgcaaaacc gtcgaaggca tctacgtcac ccccatcgtt
     1021 gagtccggcg agatcatcga gccgctgcgc gaccgcatcg tcggccgcgt atccctcgag
     1081 aagatcaagg actacgaagg ccaggtcatc gtcgacatca accaggaaat caccgaggat
     1141 ctcgcctcgg ccatccaggc tgccggcatc gagcgcgtca agattcgctc cgtcctcacc
     1201 tgcgaatccc gccgtggcgt ctgcatcctc tgctacggcc gtaacctcgg ctccggccgc
     1261 ctcgtcgaac tcggcgaagc ggtgggcgtc atcgccgcgc agtccatcgg cgagcccggc
     1321 acgcagctca ccatgcgtac cttccacatc ggtggaacgg cctcgcgtgt caatgagcag
     1381 tcgcgcctcg aagccaagaa caacggttca gtccgcttca tcaacctggc cacggtccgc
     1441 agccgtcagg gcgatctggt cgccatgaac cgctccggct ccattgccgt cgtcgatgag
     1501 cgcggccgcg agaaagagcg ctaccaggtc gtctacggcg cgcacatcaa aatcgaggag
     1561 aaccagccgg tgcagctcgg ccaggtcatg gtggagtggg atccctacac cttcgccatc
     1621 ctgaccgaaa tcggcggcac ggtcgggttc aaggacctgc aggaaggcat cacccttcac
     1681 gaagaagtcg acgaggtcac tggcctctcg cgcctcgtgg tgggcgattc ccccgatgag
     1741 aagcgccagc cggccatcgt catcaaaggc ggcaagggcc agaagcgcta tctcatgccc
     1801 agccgttctc acctcatgat tcaggacgga gacgaggtct cccccggcga catccttgcc
     1861 aagatcccgc gcgaaaccac gcgcaccaag gacatcaccg gtggtctgcc ccgcgtcgtc
     1921 gagctcttcg aagcccgcaa gccgcgcgaa accgccatca tctccgaaat cgatggcgtc
     1981 gttcgcttcg gcgaagtttc gaaaggccag cgccgcatct acgtcaccgc cgacaatggc
     2041 gaggagaagg aatactccgt gccgcgcggc gtccacatca acgtccagga aggcgagcgc
     2101 gtccgcgctg gcgagccgct catggatggt ccgctcaacc cgcacgacat tctcgccgtc
     2161 ctcggtgaga aggagctgca ggcttacctg gtcaatgaaa tccaggaagt ctaccgcctc
     2221 cagggcgtgg ccatctccga caagcacatc gaaaccatcg tccggcagat gctccgctgg
     2281 gtgaagatcg aggaagtcgg cgactccagc ttcctgctcg aacagcaggt cgacaagttc
     2341 cgcttccgcg aagagacgca gcgtgtcatc accaacggcg gccgtccgcc cgttggacgc
     2401 ccgctgctgc tcggtatcac caaggcgtcg ctctcgaccg agtccttcat ctccgcggcc
     2461 tccttccagg agaccacgcg cgttctcacc gaagccagca tcaacggcgc ggtcgacaac
     2521 ctgcgcggcc tcaaggaaaa cgtgatcgtc ggtcgcctca tccccgccgg aaccggcatg
     2581 gaatactacc gcaacgtcca gctctctccg gagctggaag aagcggcagc caaaatccag
     2641 caggaagtcc agaccgcctg ggaagaagcc gaacgcgagc tggagatgat gcgtcaggaa
     2701 ggcgaagccg aagaaatggc cgcctccgaa taacctcaac cgttgtcatc ccgaccgaag
     2761 cggtcccgca agggagcgcg cagcggaggg acctgcttct ccagcacaaa tgcccggcgc
     2821 gagccgggca tttgtgtttt catttgtcat cctgagcttc ggccacgcgc aagcgtgccg
     2881 agtcaaggat ctgcggttgc gtttcaaacc tcagctcgat gtatagtccg gagcggggag
     2941 gactgtgtca tggcagactg ctcagaatgt gatggcaccg gcaaggtgat atgcccgcga
     3001 tgtgacggag acgggtgcga attgtcaatc attcctggac taaccgatga gtgcccgcag
     3061 tgttatggtc acaaagagat agattgtccg gaatgccatg ggagcgggta cgcatgatgc
     3121 gagccatgct cgcccagggc ttcgtcggta ccgcgggaag gaaatccacc taccgattgc
     3181 aacacggcta cttcaccaag gtgaggaagc cagcgcgctt ctgcgaaacg gccttgagag
     3241 aaatgccagc gatctcagta aacacgggcc ctgagaatcc taagtcagca cacaattcgg
     3301 gaagcgtctt cttaacgtgt ccaccgagcg cgtctattgc ctgcacgaaa agctctggtt
     3361 cctgaaaatg cggctcgaaa ggttcttcgc gacgccagtt ttttgaagac atatactgaa
     3421 aggcccttcg ataagcgact tcatccagca agccgaggtc gcgagcccgc ctcactatcg
     3481 cggcaatact aacctgccac cgttgcttta ggtcaaaaac gtgctgccac gtgaagggct
     3541 tagtccggaa ctctcgacca aaggcattag cgggcagcag gaaagcactg gcaaatctgt
     3601 cggccgcgcg ttcggtttcg agcgcaccgg tcggcgtgtc acggtgcata gcaatatgcc
     3661 cgcattcatg cgccaagtca aaattccat
//
LOCUS       DASZYN010000103         7062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_133240, whole genome shotgun sequence.
ACCESSION   DASZYN010000103 DASZYN010000000
VERSION     DASZYN010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7062
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133240"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VHE33_02695"
     CDS             complement(<1..611)
                     /locus_tag="VHE33_02695"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HVT96385.1"
                     /translation="MSLRSRMTTWWRAISRADALDHQLHEELKFHIDSCAEDMVRQGV
                     PREEALRRARAELGSLPAVHENSRQAWGTRFLDELRGDLRFAIRVLARSPGFTAIAIG
                     SLALGIGANTAIFTAAQHMLLDRLDVPHPEQLRLFGWTAPRNGVVESLWGQWDDLPGG
                     GMVSTSFSFPVYLRLRRENRSLADVFAFKGFGRMVATVDGHAQT"
     gene            complement(608..955)
                     /locus_tag="VHE33_02700"
     CDS             complement(608..955)
                     /locus_tag="VHE33_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVT96386.1"
                     /translation="MSGDTAIDLLQGTLDMLVLKAVSLGPLHGYGVLLRIQQISQNRL
                     EIQQGSLYPALYRLEHEGWIAGEWGQSENKRRARFYRLTAAGRRRLDTETKKWSQFAE
                     VVGTILSTRPEEA"
     gene            1280..3655
                     /locus_tag="VHE33_02705"
     CDS             1280..3655
                     /locus_tag="VHE33_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HVT96387.1"
                     /translation="MVLLFAACWSCAWARIHTDETANPQTGKQAAAPHATKHHATPSN
                     AHTSHRASARTTHTSRHRSVSHRRKRRHVLTARERARSRRYQHAFVASSQLRPMAQQL
                     AQNPTPAAFAGVNAWAHGHTGEAAAAAYLAVGHAYLLQSKFPEAVAALHSADRADHTS
                     TEPDGALPDYVEYLTAQAYLQSNQLPQAETVLDRFDRKYPDSIFVDSVPVLRANLLVQ
                     QGNAQGALAVLNPLRQQSIGGKADFQLALAKAAALAGNSSEAQRLYEHIYLDYPLSTE
                     AGVARGQLAAIGALAALPSEQRRRHADALYNAGHYSDAEDEYRSLAGQSNIDEASRTA
                     LLVAAAECDWKQHRLSQSQLDSIPDTNDEAGARRMYLQMELARDRGDQSAQQAIVSQM
                     ETRFPSSSWLAEALYSSGNMYLLLKDYPHAIGYYGEVARRFPTHRYAPSAHWRTGWLN
                     YRLGQYAEAARLFDEQIRVYAGGKEVPSAIYWRGRIYEDQEKKPADAAEYYQTILRVY
                     PHYYYAMLAQDRLTKVGSVTPDPNPVLDALQPTTIPELSDDVPEDDPHVVKARLLANA
                     GLNEYIAPEIRAADGSDGWGAFAEAEIYASYGESWRAMRLLKRAIPFYTSAPIEAIPV
                     AYWRILFPQSYWSDIKADAERNGLDPYMVAALIRQETEFNPGAISNKSAYGLMQLLPS
                     VGRSMARQVGLRHFQTSELLNPSTNIRLGTLYLKQTLEKFGGQPEYAFAAYNAGDDRV
                     TDWQSIGNYHGMDEFVESIPFTETREYVEAILRNEQIYRELDKATDQRAER"
     gene            3796..5892
                     /locus_tag="VHE33_02710"
     CDS             3796..5892
                     /locus_tag="VHE33_02710"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HVT96388.1"
                     /translation="MIRHRPASTSGPAWAAMFALAAFLPIAVNAQEKGTATVTNAQHG
                     IAVSHIDTAVKPGDDFYRYANGAWIAGTEIPADRASIGVFAILADHSSKNIAAIIEEA
                     AKSRAATGSNTRKIADLYNAYMDEAAIESRGLRPLQSEMAAIAAIHDRHDLAGVLGKT
                     LRADVDMLNNGIFSTPNLLGLWVAPGFSDSDHYTPYLLQGGLEMPSRSYYLDDSQHMK
                     DLRVQYSAHVARMLRLAGFDDSEARASRIIQLEHDIAATHVSLSDIEDIAKANNRWTR
                     ADFTEKAPGLDWNAYFDAAGLAQQQQFMVWTPTALTGEASLVASVPVETWKDWLRLHL
                     VEAYANVLPRALAEEQFSFFGKALSGVPQQRPRWQRGVNMVNADLGDAVGEIYAKKYF
                     SPEAKAEAQAMVANLIAAYRKRIDQLTWLAPSTKAEAQAKLNTLYVGIGYPETWKDYS
                     AYEVRPDDAFGNAWRASMWDYHREIARIGKPVDRHEWAMEPQTVNAVNLPLQNALNFP
                     AAILQPPFFDPKAPAVVNYGAIGGVIGHEISHTFDAEGSAFDSKGRVRNWWTPEDHAH
                     FEQQAEKLEKQYDAYEPFPGLHVNGKQTIDENIADLGGIAAAYDGYRASQHGGQAPVQ
                     DGFTGDQQFFIAFAQNWASKTREAALRRQVLTDGHSPGQYRADIVRNSDAWYKAFDVK
                     PGETLYLAPDERVRIW"
     gene            5984..>7062
                     /locus_tag="VHE33_02715"
     CDS             5984..>7062
                     /locus_tag="VHE33_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96389.1"
                     /translation="MKNLWCCRAPRRIALAIAAVSLAASPSIGAAQSERESPHPPAAA
                     APQAAKPGPNPVAEKNPLPPLPPDAHVAQSITIDGKKINYTATVGTLFVRDQEGKAVG
                     QLVYTAYTVDGANRPVTFAVNGGPGAASVYLNFGAIGPKHLNAGNEGDSPSDPATLVD
                     NPGTWLDFTDLVFLDPIGTGFSRSLVDEDQTKKLFWSTEPDIEYLSRAIYDWLVLNDR
                     LQSRKYLVGESYGGFRGPRLTYFLQSQLGVAMNGVVLVSPYLNPTLEQNGDLSPLPWL
                     VTLPSITAANLERQNKLTPEAMKGVIDYTLGDYLTTIVHGYSDQAATDAMIKKVTDMT
                     GLDPTFVKESGGRLETRAYLREVHR"
BASE COUNT         1398 a         2117 c         2257 g         1290 t
ORIGIN      
        1 gtttgcgcat gcccatcgac ggtggcgacc atgcgtccga aacctttgaa ggcaaagacg
       61 tcggccagcg agcgattctc gcggcgcagc cgcagataca ccgggaagga gaacgaggtt
      121 gaaaccatac cgcccccggg cagatcgtcc cactggcccc acaaggattc gacaacacca
      181 ttgcgcggtg cggtccagcc gaagaggcgc agctgttccg gatgcgggac gtccaaccgg
      241 tcgaggagca tgtgctgcgc tgcggtgaag atagcggtgt tggcgccgat gccgagggcg
      301 agcgagccga tggcgatagc ggtgaagccg ggactccttg ccagaacacg aatcgcgaag
      361 cgcagatcgc cgcgcaactc gtcgaggaaa cgcgtgcccc aggcctggcg ggaattctcg
      421 tgcacggcgg ggagactacc gagttcggcg cgggcacggc gcagagcttc ttcgcgcgga
      481 acaccctggc gcaccatgtc ctcggcgcag ctatcgatgt ggaacttcaa ctcctcatgg
      541 agttggtggt cgagcgcatc cgcgcgggag atggctcgcc accaggttgt catgcggctg
      601 cgcaggctca tgcttcctcc ggccgtgtgc tgagaatggt cccgaccacc tcggcgaact
      661 ggctccactt cttcgtctct gtgtcgagcc ggcggcggcc ggctgccgtg agccggtaga
      721 agcgggcgcg gcgtttgttc tcgctctggc cccattcgcc ggcgatccag ccctcgtgtt
      781 ccagccgata gagcgccgga taaagggagc cctgctggat ctcaaggcgg ttctgcgaaa
      841 tttgctggat gcggaggagc acgccgtagc cgtgcagagg gccgagggag acagccttca
      901 ggaccagcat gtccagcgtt ccctgcaata gatcgatggc tgtgtcgccg ctcatgttgt
      961 tctcccagat tgtctgggag agtagccggg cttctcccag actgtcaagg agagtgtgac
     1021 gaaagccaaa tggcgagcga gaagcgagcg aaaaagcgac ggaagggcgt ggctgaagcg
     1081 ggggaggagc gcgttcgcgc gatctagacc gctgaaagag ccgcccagga aactattccg
     1141 gtaccgcctg ttgcaacttt ttggtcaggc gcagggtttt cgttcgcgaa tgccgattcg
     1201 tttcgtctta ctctggtagt cagaggggtg gatgttggta ttgcgtcgtc gtcaatttgc
     1261 gagtgcgtgt tctgtggcaa tggttttgct gtttgctgcc tgctggagct gtgcctgggc
     1321 acgcatccac acggatgaaa ccgcgaaccc gcaaacagga aaacaagcgg cagcccctca
     1381 cgcgacgaag catcacgcaa cgcccagcaa cgcccacacc tcgcatcgcg cctcggcgcg
     1441 aaccacccac acatcgcgcc accgcagcgt gagtcatcgc cggaaacggc ggcatgtgct
     1501 gactgcccgg gaacgcgccc gcagccgccg atatcaacac gcgttcgttg cttcttcgca
     1561 actgcgcccc atggcgcagc aactggcgca gaacccgaca cccgccgcgt ttgccggagt
     1621 gaacgcatgg gcgcatggtc atacgggaga agccgcagcc gctgcgtatc tcgccgtagg
     1681 tcacgcatat ctgctgcaga gcaagtttcc cgaggccgtg gccgcgctgc attcggccga
     1741 tcgtgccgat cacaccagca ccgaacccga tggcgcactg cccgactacg tcgaatacct
     1801 cactgcgcag gcgtatctgc agagcaatca gctgcctcag gcggaaactg tgctggatcg
     1861 attcgatcgc aagtatccgg acagcatctt tgtggacagt gttccggtcc ttcgggcgaa
     1921 tctgctggtg cagcaaggca atgcccaggg tgcgctggct gtgctgaatc cactccggca
     1981 gcagtcgatt ggcggtaagg ccgactttca gctggctttg gccaaagcgg cggcgctggc
     2041 cggcaacagt agtgaggcgc agcggctgta cgagcatatt tacctggact atccgctctc
     2101 gaccgaggcc ggggtagcgc ggggtcaact cgcggcgatc ggtgcgctgg ccgctctgcc
     2161 atcggagcag cgccgccgcc atgccgacgc actctacaac gccggacatt attcggatgc
     2221 agaggatgaa taccgctcgc tcgcggggca atcgaacatc gatgaagcca gccgcacagc
     2281 gctgctggtt gcggccgccg agtgtgactg gaagcagcac cgtctgtcgc agtcgcagct
     2341 ggactcaatt cccgacacga acgatgaagc cggcgcgcgg cgcatgtatc tgcagatgga
     2401 actggcacgg gatcgcggcg atcagagcgc acagcaggcg atcgtgagtc agatggagac
     2461 gcgcttccca tcgagttcct ggctggccga agcgctttac tcgagcggca acatgtattt
     2521 gctgctgaaa gactatccgc acgcgatcgg gtactacggc gaagtggcgc gccgcttccc
     2581 gacgcaccga tatgcgccaa gcgcgcactg gcgcaccggc tggctgaact atcgtctggg
     2641 ccaatatgcg gaggccgcgc gtctgttcga tgaacagatt cgcgtctacg ccggaggcaa
     2701 agaggtgccg tcggcgattt actggcgtgg acgcatctac gaggaccagg agaagaagcc
     2761 ggcggatgca gccgagtatt accagaccat ccttcgtgtt tatccgcact actattacgc
     2821 gatgctggcc caggaccggc tgacaaaagt cggttcggtg acgccggatc cgaacccggt
     2881 cctcgacgcg ctgcagccga cgacgattcc tgagctgagc gacgatgtgc ccgaagacga
     2941 tccccacgtg gtgaaggcgc ggttgctggc gaatgccgga ctgaacgaat acatcgcgcc
     3001 ggagattcgt gccgcggacg gcagcgatgg gtggggcgcg ttcgctgagg ccgaaatcta
     3061 tgcgtcgtac ggcgaatcgt ggcgggcgat gcgactgctg aaacgagcga ttccgttcta
     3121 cacctcggct ccaatcgaag cgatcccggt ggcgtattgg cggatcctgt tcccacagtc
     3181 gtactggagc gacatcaagg cggatgccga acgcaacggg cttgatccgt acatggtcgc
     3241 ggcgctcatt cgccaggaga cggaattcaa tccaggcgca atttccaaca agagcgccta
     3301 tggcctgatg cagttgctgc cttcggtggg gcggtcgatg gcgcgccagg tggggctgcg
     3361 ccattttcaa acgagcgagt tgctgaaccc atcgacgaat attcgtctcg ggactctcta
     3421 tctgaaacaa acgctggaga agttcggcgg gcagccggaa tatgccttcg ccgcctataa
     3481 cgccggggac gatcgggtca cggactggca gagcatcgga aattaccacg gcatggacga
     3541 gtttgtggag tcgattccgt ttacggaaac ccgcgaatat gtcgaggcga tcctgcgcaa
     3601 cgagcagatc tatcgggaac tggacaaggc gacagatcag cgcgccgagc gctgactggc
     3661 aggagagagg cccggccact ccccatccca tttgtgaccg cggccgtcac atactgcgac
     3721 gaaagctgct ggtttcgcat gctatgatcc tgcttcgccg atcgtcacca cggcattcat
     3781 tcacgaggag ttgccttgat ccgccatcgt cctgcttcca cttccggtcc tgcctgggca
     3841 gccatgtttg cactggccgc ttttctgccg attgcagtga atgcccagga gaaaggcacc
     3901 gcgactgtca caaatgcgca gcacgggatt gccgtctcgc atatcgacac cgcggtgaag
     3961 ccgggcgacg atttctaccg ctacgccaac ggagcctgga tcgcaggcac ggagattcca
     4021 gcggaccgtg caagcatcgg cgttttcgcc atccttgccg atcacagcag caagaacatt
     4081 gccgcgatca ttgaagaggc ggcgaagtca cgggctgcca ccggttcgaa cacccgaaag
     4141 atcgccgacc tctacaacgc ctacatggat gaggctgcca ttgagtcgcg cggactgcgg
     4201 ccgctccagt ccgagatggc tgcgatcgcc gccattcacg accggcacga tctggcaggg
     4261 gttttgggaa agacactgcg cgccgatgtg gacatgctga acaacgggat cttttcgacg
     4321 ccgaacctgt tggggctttg ggtcgcgccg ggattcagcg attcggatca ttacacaccc
     4381 tatctgctgc agggcggcct ggagatgccg agccgcagct actatctcga cgactcgcag
     4441 cacatgaagg atctgcgggt gcagtactcc gcgcatgtgg cgcgcatgct gcgtctcgcc
     4501 ggctttgacg attcggaagc gcgcgccagc cgcatcattc agctggagca tgacatcgct
     4561 gcgacccacg ttagcctttc cgacatcgag gacatcgcaa aggccaacaa tcggtggaca
     4621 cgcgccgatt tcaccgagaa ggcgccgggg ctggactgga atgcgtattt cgatgccgcg
     4681 gggctcgctc agcagcagca attcatggtg tggacgccaa cggcgttgac gggcgaagcg
     4741 tcgctggtgg cctcggttcc ggtggaaacg tggaaggact ggctgcgctt gcacctggtt
     4801 gaggcctatg ccaacgtgtt gccgcgtgca ctggcggagg aacagttctc cttctttggc
     4861 aaggcgcttt caggtgtgcc gcaacagcgt ccgcgctggc agcggggcgt gaatatggtg
     4921 aatgcagatc tcggcgatgc tgttggtgag atttacgcga agaagtattt ctcacccgag
     4981 gccaaagccg aagcccaggc gatggtggcg aacctgattg ccgcgtatcg caagcggatc
     5041 gaccagctca cctggctggc gccctcgacg aaggcagagg cccaggccaa gctgaacacg
     5101 ctctatgtcg gcatcggata tccggagaca tggaaggact actcggccta tgaggttcgg
     5161 ccggacgatg cgtttggcaa tgcgtggcgt gcttcgatgt gggattacca tcgcgagatt
     5221 gcgcgcatcg gcaaaccggt cgatcgccac gagtgggcga tggaaccgca gacggtgaac
     5281 gcggtgaatc tgcccctgca gaacgcgctc aatttcccgg ccgcgatcct gcagcctcca
     5341 ttcttcgatc cgaaggcgcc ggccgtggtg aactacgggg cgattggcgg cgtgatcggg
     5401 catgagatca gccacacgtt cgacgcagag ggaagcgctt tcgattccaa aggccgcgtg
     5461 cgcaactggt ggacccctga ggatcacgcg cactttgaac agcaggcgga gaagctcgag
     5521 aagcagtacg acgcgtatga gccgttcccc ggccttcacg tgaacgggaa gcagacgatc
     5581 gatgagaata tcgccgatct gggcgggatt gccgccgcct acgacgggta tcgggcatcg
     5641 cagcatggcg gccaggcgcc cgtgcaggac ggattcacgg gggatcagca attttttatc
     5701 gcattcgcgc agaactgggc ctcaaagacc cgggaggccg cgctgcggcg gcaggtgctg
     5761 accgacggtc attcgccggg gcagtatcgc gcggatatcg tgcgcaattc ggatgcgtgg
     5821 tacaaagcgt tcgacgtgaa gccaggagag acgctctacc tcgcgccgga tgagcgagtg
     5881 cgcatctggt agacagtctc tgccgttcga atggccatgg tgttttgcga gtcctgagcg
     5941 actagcgccc cgctgggtcg cattccgata tatcgtattc gtcatgaaaa atttgtggtg
     6001 ttgtcgcgcc cccaggcgca ttgctctggc cattgccgca gtgtctctcg cagcatcgcc
     6061 cagtatcggt gccgcacagt ctgaacgcga atctccccat ccgccggcgg ccgctgctcc
     6121 tcaggcagcc aagcccggcc ccaatcccgt tgccgagaag aacccgctgc cgccgctgcc
     6181 gcccgacgcg cacgtcgcgc agagcatcac gattgacggc aagaagatca actacaccgc
     6241 cactgtcggc acgctgttcg tgcgcgatca ggaaggcaag gccgtcgggc aactggtcta
     6301 taccgcgtac accgtcgacg gggccaatcg tcctgtgacg tttgcggtga acggcggacc
     6361 cggcgcggcc tcggtctatc tgaacttcgg cgccattggc cccaagcacc tgaacgcggg
     6421 caacgaaggc gacagcccat ccgatccggc aacgctggtg gacaatccag ggacgtggct
     6481 ggacttcacg gaccttgtgt ttctcgatcc catcgggacg ggcttcagcc gttcgctggt
     6541 cgatgaggac cagacgaaga agctgttctg gtcgaccgag ccggatatcg agtatctgtc
     6601 gcgggcgatc tatgactggc tcgtgctgaa tgatcgcttg cagtcgcgca agtatctcgt
     6661 cggcgaaagt tacggaggat tccgcggacc ccgcctcacc tacttcctgc agtcgcagct
     6721 gggcgtggcc atgaacgggg tcgtgctggt ctcgccttac ttgaacccga cactggaaca
     6781 gaacggagat ctgtcgccgc tcccgtggct ggtgacgctg ccttcgatca cagcagccaa
     6841 tctggagcgg cagaacaagc tgactccaga ggcgatgaag ggcgtcatcg actacacgct
     6901 gggtgactac ctgacgacca tcgttcacgg atacagcgat caggctgcga ccgacgcgat
     6961 gatcaaaaag gtcaccgaca tgacggggct cgatccgacg ttcgtgaagg aatccggcgg
     7021 gcggctcgaa acccgcgcgt acctgcgcga ggttcaccgc ga
//
LOCUS       DASZYN010000104         9573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1225573, whole genome shotgun sequence.
ACCESSION   DASZYN010000104 DASZYN010000000
VERSION     DASZYN010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 9573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9573
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225573"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1679)
                     /locus_tag="VHE33_02720"
     CDS             complement(<1..1679)
                     /locus_tag="VHE33_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HVT96390.1"
                     /translation="MASGVQHKAEYDAEHRVITAGGIVKTGPVVFQDTAKAAGLTTWH
                     HIAGTPDKPYIVEAKGPGVCLIDYDNDGWLDIYFVNGSTLDAMNGKAAAPKAALFHNN
                     HDGTFTDVTEKVGVANERWGYGCAVADYDNDGWPDLFVSNYGQNRLYHNNHDGTFTDM
                     AEKAHVELGTWSTGATFGDYDGDGHLDLFVAGYIHFDFVHPPVSGSRTVNYANCQYRG
                     VETMCGPRGLPGERDHLFHNNGDGTFTDVSEKLGVDNKNGYYGLGALFVDVNNDGKPD
                     LIVANDSTPNYLYINKGNGTFEDDSYLSGLALNADGREAANMGIAAGDYENNGHMDIV
                     TTTFADDYDIVFNNDGKGNFTDVAYPSGVAAPTIPYVSFADGFLDYDNDGWLDLFFTN
                     GHVYPVVDEHPEWGTTYEEKPLLFHNEKGGKFALIPAVAGTGLAVVTVGRGAAFGDLF
                     NDGKIDVVMNNMDGVPVLERNVSPDHHHWVELKLVGGPKSPKDAVGSTVYLTAGGIRQ
                     RGDVLSGGSYLSSNDPRVHFGLGDAAKVDAVEIHWPDGKVEKVTPPEVDKIV"
     gene            1941..4139
                     /locus_tag="VHE33_02725"
     CDS             1941..4139
                     /locus_tag="VHE33_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HVT96391.1"
                     /translation="MPVRRIAVLSALVCCCSGLTLLFASQKPAPVHVPTAVLDSYVGQ
                     YREQDEPDIVLSVFRDGDRLYMESTRTARLYLTAKSSTAFEPDFNATYTFVTDSSGAV
                     TAMKFSAQGSPDSILTRISSTPVHNHFRPYSREEVMIPMRDGVKLHAVILRPTDTREP
                     LPFLMQRTPYGVDGYDSDAINGRYTELAQSGYIFVMEDIRGRYKSEGKFVMMRPLADH
                     SDAKAVDESTDSNDTVAWLLEHIAHNNGRVGVMGISYPGFLAAEAGIDPNPAVRAISP
                     QAPMTDVWMGDDFFHNGAFRQTYGYDYVIGMESSKENAFNKLNEDAYTFFLNAGSFEA
                     AGKLSDQMSGGSAGVDDLPTWRAFLDHPAYDGFWKSRAVQDHLTKIAVPTLEVGGYWD
                     QEDLWGPQEEYAVLEPHNEPGNPAHRVYMVLGPWRHGGWSQTTRRLGDVNFGEPVGDE
                     FRRQIEAPFFAYYLKDQPGFNLENTATYQTGSERWMRYGQWPPKNVTTRDLYLDEDGS
                     LDFDKPTDTNSFKAFTSDPADPVPYRRRPIEATYAPSGSGWYTWLVQDQGFVNGRKDV
                     ASWETAPLDHDVTVTGNVVADLIASTSGTDSDWVVKLIDVFPEKPAAEARQEPCLAID
                     PGLGAKPAGFELMIADEIFRGRYRTSFSKPEAIPANTPEEYKFSLHAADHVFLKGHRI
                     MVQVQSTWFPLYDRNPQTFVPNIMTARPSDYKSAEQRVYSGSHIELPEPQ"
     gene            4204..5520
                     /locus_tag="VHE33_02730"
     CDS             4204..5520
                     /locus_tag="VHE33_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3863 domain-containing protein"
                     /protein_id="HVT96392.1"
                     /translation="MAAASRLNGSSLLSLAAAPSPFRGRFLTHVSIVRVNQIEVSTDR
                     SIGQDESADNSPAAIRARREAFARGCPDGRMTWAISWLALHDTRQEYKDARRLLASFH
                     DRYGDEITFIPGGYFAPMYNTREQIRITIHEALAEVSKMVGNGYRPQCLVAGFMDAEN
                     QRLLAADESIHVCQGQIWSQHGIDNGDGDGGICYPYYPSRQHYLKPAQGRADFIDCVC
                     LDGWTCDFLSARRNGFEGGFNSRMGVGPIETVQDLGQQVGRKEMIDTTAMHFDGGHAL
                     NGFGWVTAIWEVSIGHDPDLTWWLQAVRERWPDTRVITEGEFGLEWRRHFPNNDALNY
                     RFDAKGTGAPGSEKNLEVLWYMNQKFRLALLHDWTTSSPRMVIDFTRYDLPAREPQKL
                     QREWSLMNVLNQKGTRPQDKPTRLGQLPPEDQRIIYAHYPDLKSWA"
     gene            complement(5604..7568)
                     /gene="acs"
                     /locus_tag="VHE33_02735"
     CDS             complement(5604..7568)
                     /gene="acs"
                     /locus_tag="VHE33_02735"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HVT96393.1"
                     /translation="MGSEIAINPNLETILREHRVFPPPEEFAKQAHVKNLAEYQRMYQ
                     RSVGDPEGFWADAAKELHWFKPWEKVLDWNLPWAKWFVGGKINLCYNCVDRHVVNGNA
                     DKVAILWEGEPGEVRKLTFADLHSEVQKFANVLKGLGIKKGDRVAVYMGMTPELAIAI
                     LACARIGAVHSVIFGGFAANAIVDRVNDAQCVAILTQDTSYRRGNEVKLKATVDEAVG
                     QCPTVKNVVVYKRTGSPVNMEAGRDHWWHELMKAAEKECPAEELDSEDPLYILYTSGT
                     TGKPKGLVHTTGGYSVQTYLTSKYIFDLREDDVYWCTADIGWVTGHSYVVYGILQNGT
                     TTVMYEGAPNHPTLDRFWKIIDDHKVTVFYTAPTAIRAFIKWGDEYPAKHKMDSLRLL
                     GTVGEPINPEAWIWYREVIGKGRCPIVDTWWQTETGGIMISPIPGAVAAKPGSATRPF
                     FGIVPEVVTKEGQPVPAGQGGLLVIRKPWPSMARTIWGDPDRYVKQYWSDVPGCYFTG
                     DGARMDEDGYFWLMGRVDDVINVSGHRLGTMEVESALVAHPKVAEAAVVGRPDELKGQ
                     AISAFVTLEGGNTPTKELKDELRAWVAKEIGSLARPDDIRFTDALPKTRSGKIMRRLL
                     REMATHGEIKGDVTTLEDFSVIAKLREQDE"
     gene            7671..8426
                     /gene="kdsB"
                     /locus_tag="VHE33_02740"
     CDS             7671..8426
                     /gene="kdsB"
                     /locus_tag="VHE33_02740"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HVT96394.1"
                     /translation="MPSNLGHVLAVIPARLGSTRLPRKVLREIAGEPMIAWVYRAARA
                     CPLLDRVLIATDSDEVLQFAQQHLLPAVFTPVDCASGTDRVWAVAQSIPADIYVNIQG
                     DEPLLTPEHFTPLLQLFERPEVQVATLAVRCPQHDVNNPSAVKVVTAADGRALYFSRA
                     TIPFDRDNSGFAGYRKHLGVYAYRKAALQKFANTPPSWIEQIERLEQLRLLDNGIDIH
                     VAPAPSDTIGVDTEEDLRAAEAVILSEGRPRRP"
     gene            complement(8452..>9573)
                     /locus_tag="VHE33_02745"
     CDS             complement(8452..>9573)
                     /locus_tag="VHE33_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT96395.1"
                     /translation="RVARTDVAAAISITLMFRPVGAFVFGVLADRFGRRPTLMFNIAS
                     YSLLELACAFTGSMHELFILRALFGIAMGGEWGVGAALAFETLPKENRGFFSGLLQEG
                     YAVGYLVAAAAYALLFPWFSHFTWHGHAFGWRGMFLVGAAPALLVFYIGGKVEESPTW
                     RERGELEERTIPVMGLRKGLLQYAGTFVFLVLLMTGFNAFSHGTQDLYPTFLQKEHGF
                     SAQLTGAIAILYNIGALAGGMVFGTFSEKVGRKRAIILAALLALPAIPLYALSHSVWM
                     LAAGAFVMQFMVQGAWGVVPAWLSELSPGPVRATFPGLAYQLGNLLASWNAVIQTRAA
                     ERLGGLGPVLAVTVAIVACYVALVAAFGRESRGVDLRAT"
BASE COUNT         1815 a         3253 c         2670 g         1835 t
ORIGIN      
        1 acgatcttgt ctacctccgg cggcgttacc ttctcgacct tgccgtccgg ccagtgaatt
       61 tccacggcgt ccaccttcgc ggcatcgccc agcccgaaat ggacgcgggg atcgttcgac
      121 gacagatagc tcccgccgct cagcacatcg ccgcgctgcc ggatgccgcc cgcggtcaga
      181 tacaccgtcg agccgaccgc gtccttcggg ctcttcgggc cgccaacgag cttgagttcc
      241 acccagtgat gatgatccgg gctcacgttg cgctccagca cgggcacgcc gtccatgttg
      301 ttcatcacca catcgatctt gccgtcgttg aagaggtcgc cgaacgccgc gccgcgtccc
      361 accgtcacca ccgccagccc ggtgccggcc acggccggga tcagcgcgaa cttaccgccc
      421 ttctcgttgt ggaagagcag cggcttttcc tcataggtcg tgccccattc cgggtgctcg
      481 tccactaccg gatagacgtg cccgttggtg aagaacagat cgagccagcc atcgttgtcg
      541 tagtccagaa acccgtcggc aaaactcaca tacggaatgg tcggcgccgc gacgccggag
      601 ggataggcaa cgtcggtgaa gttgcccttg ccatcattgt tgaagacgat gtcgtagtca
      661 tcggcaaagg tcgtggtgac gatgtccatg tgcccgttgt tctcgtaatc gccggcggcg
      721 atgcccatgt tggccgcctc gcgcccgtcg gcattgagcg ccaggccact caggtagctg
      781 tcgtcctcga aggtgccatt gcccttgttg atgtagagat aatttggggt cgagtcgtta
      841 gcgacgatca ggtcgggctt gccgtcgttg ttcacgtcga cgaacaacgc gcccaggccg
      901 tagtagccgt tcttgttatc gacgccgagt ttctcgctca cgtcggtgaa cgtgccatcg
      961 ccgttgttat ggaataaatg atcgcgctcg cccggcaggc cgcgcggacc gcacatcgtc
     1021 tctacgccgc gatactggca gttcgcatag ttcacggttc gcgagccgga aaccggcgga
     1081 tgcacaaagt cgaaatggat gtagcccgcg acgaacaggt cgaggtgccc gtcgccgtcg
     1141 tagtcgccaa aggtggcgcc cgtggaccac gtgcccagct ctacgtgcgc cttctcagcc
     1201 atgtcggtga acgtgccgtc atgattgttg tggtagagcc ggttctgtcc atagttgctg
     1261 acgaacaggt ccggccagcc atcgttgtcg taatctgcca cagcgcagcc atagccccag
     1321 cgctcgttgg cgacgccgac tttctcggtc acatcggtga aggttccgtc gtggttgttg
     1381 tggaacaatg ccgccttcgg cgccgcggct ttcccgttca tcgcatccag cgtcgacccg
     1441 ttgacgaagt agatgtccag ccatccgtcg ttgtcatagt cgatgaggca gacgcccggg
     1501 cccttcgctt ccacgatgta aggtttatcc ggcgttcccg caatgtgatg ccacgttgtc
     1561 agtcccgccg ctttcgctgt gtcctgaaac accaccggac cggtcttgac gatgccgcct
     1621 gccgtgatca cacgatgctc cgcgtcgtac tcggccttgt gctgaactcc gctcgccatg
     1681 cccgtgttct gagctccgct ctgcggttcc tgcgctgccc ctgctgccca ggcaaacagg
     1741 aaacatcctc ccaccaaccc caggcagaac tgatttgcca gtccactgaa cgctgaacgc
     1801 tgacagctga cagctgccgt cctgatcccc actcggtact tcccttctct ggaattcagc
     1861 caccattgtc tcccgccgcc atcgctttgc gaaagccccg gcatcatcgc tcccgctgag
     1921 ccgcgatacc atgaattccg atgcccgtcc gccgaattgc cgtcctgtct gccctggtct
     1981 gctgctgttc cggtctcacg cttctgtttg cctcgcagaa acccgctccg gttcatgtcc
     2041 cgaccgccgt tctggacagc tatgtgggtc agtatcgcga gcaggatgag ccggacatcg
     2101 tcctctcggt cttccgcgac ggcgatcgcc tctacatgga gagcactcgc accgcgcgcc
     2161 tgtacctgac ggcgaaatcc agcaccgcct tcgagcctga cttcaacgcc acgtacacgt
     2221 tcgtcacgga ttccagcggc gccgtcaccg cgatgaagtt ttccgcacaa ggctcgcccg
     2281 acagcattct tacccgcatc tcctcgacgc cggtgcacaa ccactttcgc ccttactcgc
     2341 gcgaagaagt catgatcccc atgcgcgacg gcgtcaaact ccatgctgtg atcctgcgcc
     2401 ctacggatac cagggagccg cttcccttcc tcatgcagcg gacgccctac ggcgtggacg
     2461 gctacgactc cgacgcgatc aacggacgct ataccgagct tgcgcagagc ggctacatct
     2521 tcgtgatgga agatatccgt ggccgttaca aatccgaagg caaattcgtc atgatgcgtc
     2581 cgctggccga tcacagcgat gcgaaagctg tggatgaaag cacggacagc aacgacaccg
     2641 tcgcctggct gctcgagcac attgctcaca acaacggtcg cgtcggggtg atgggcattt
     2701 cgtatcccgg atttctcgcc gcagaagccg gcatcgaccc gaaccccgcc gttcgcgcga
     2761 tctcaccgca ggctcccatg acggacgtgt ggatgggcga cgattttttt cataacggcg
     2821 ccttccgcca gacctacggc tacgactacg tcatcggcat ggaatccagc aaggaaaatg
     2881 cgttcaacaa actgaatgag gacgcctaca ccttcttcct gaacgctggt tcgttcgaag
     2941 ccgccggcaa gctctccgat cagatgtccg gcggatcggc gggggtcgac gacctgccca
     3001 cctggcgcgc ttttctggat cacccggcgt atgacggttt ctggaagtca cgagcggtcc
     3061 aggatcatct gacaaaaatc gccgtgccca cgctggaagt cggcggttac tgggaccagg
     3121 aagatctgtg gggaccgcag gaagaatacg cagtgctgga gccgcacaat gaacccggca
     3181 atccagcgca tcgggtctat atggtcctgg gaccgtggcg gcacggtgga tggtcacaga
     3241 ccacgcgtcg gctgggcgac gtcaacttcg gtgagccggt tggcgacgaa ttccgccgcc
     3301 agattgaagc gccttttttc gcgtactacc tcaaagacca acccggtttc aaccttgaaa
     3361 ataccgcaac ctaccagacc ggatccgaac gctggatgcg ctacggccag tggccgccca
     3421 aaaatgtaac aacgcgcgat ctttatctgg acgaggatgg atcgctggac ttcgacaagc
     3481 ccaccgatac gaattcgttt aaggccttca cgtcagatcc cgccgatccg gttccatatc
     3541 gccgccgccc catcgaagcc acctacgcgc cgagcggatc cggctggtac acgtggctgg
     3601 tgcaggatca gggcttcgtc aacggacgca aagatgtcgc cagctgggag acggcgccgc
     3661 tcgaccacga cgtgaccgtg accggcaatg ttgttgcgga cctgatcgcg tccacctccg
     3721 gcaccgacag cgactgggtc gtcaagctca tcgacgtgtt tccggagaaa cctgcggccg
     3781 aagcccgtca ggagccttgc ctggcgatcg atccaggcct aggcgcgaag cctgccggat
     3841 tcgaattgat gattgcagac gaaattttcc gcggacgcta tcgcaccagc ttttcaaaac
     3901 ctgaagccat ccccgccaac acaccggagg aatacaagtt ctcgctccac gcggccgatc
     3961 atgtctttct caagggtcac cgcatcatgg ttcaggtgca aagcacctgg tttccgctct
     4021 acgaccgcaa tccgcagacg tttgtgccga atatcatgac cgcccggccg tcggattaca
     4081 aatccgccga gcagcgcgtc tattccggat cgcacattga gttgcccgaa ccccagtaag
     4141 attgcgagat ccatgcgtca tcatccctgg acccgaagag agtttcttgc aggagcagga
     4201 gtcttggctg ccgcctcgag actgaacgga agcagccttc tgtcgctcgc tgcggcccca
     4261 tcgccgtttc gcggacgctt cctcacgcac gtgtccatcg tgcgcgtgaa tcagattgag
     4321 gtcagcaccg accgctcgat cgggcaggac gaatccgccg acaacagtcc cgcggccatc
     4381 cgcgcgcggc gcgaggcctt tgcccgcggt tgcccggacg gccgcatgac ctgggccatc
     4441 agttggctcg cgctccacga cacgcgtcag gaatataagg atgcgcgccg gcttctcgcg
     4501 tccttccacg atcgttacgg cgacgagatc acctttattc ccggcggata cttcgcgccg
     4561 atgtacaaca cgcgcgagca aattcggata acgattcatg aagcgcttgc cgaggtctca
     4621 aagatggtcg gcaacggcta tcgtccgcaa tgcctggtcg ccggcttcat ggatgccgag
     4681 aatcagcgcc tgctcgcggc cgacgaatcg atccacgtct gccagggcca gatctggagc
     4741 cagcacggga tcgataacgg cgacggcgac gggggcatct gctatcccta ttaccccagc
     4801 cgccagcact acctcaaacc ggcgcagggt cgcgccgatt tcatcgactg cgtgtgcctc
     4861 gacggctgga cctgcgattt tctctccgca cgccgcaatg ggtttgaggg aggattcaac
     4921 agccgcatgg gcgtcggccc cattgaaact gtgcaggatc tgggccagca ggtgggccgc
     4981 aaagagatga tcgacaccac cgcgatgcac ttcgatggtg ggcacgcgct gaacggcttc
     5041 ggctgggtca cagcaatatg ggaagtttcc atcggccacg atccggacct cacctggtgg
     5101 ctgcaggcag ttcgcgagcg ctggccggat acccgcgtga tcaccgaggg cgaattcggc
     5161 ctcgagtggc gtcgccactt ccccaacaac gacgcgctta actatcgatt cgacgcaaaa
     5221 ggaaccggcg cacccggatc ggagaagaac ctcgaagtcc tctggtacat gaaccagaaa
     5281 ttccgcctgg ctctgctgca tgactggacg acatcgagcc cccgcatggt catcgacttt
     5341 acccgctacg atctgccggc tcgcgagccc caaaaactac agcgtgagtg gagtctgatg
     5401 aatgtcctga accagaaggg cacgcgcccg caggacaagc ccacgcgcct cggccagctg
     5461 cctccggaag atcaacgcat catctacgct cactaccctg atctaaaaag ctgggcgtag
     5521 ccaggaactg cgttcgtttc ccgacgaaaa gagcgccgtc tccggcgctc ttttctattc
     5581 cctattggct actccgtgct tctctactcg tcctgctcgc gcagcttcgc gatcacgctg
     5641 aaatcttcca gcgtcgtcac gtcgcccttg atctctccgt gcgtcgccat ttcacgcagc
     5701 agccgccgca taattttgcc ggagcgcgtt ttcggcagcg catcggtgaa gcggatgtca
     5761 tccggccgcg cgagcgatcc gatctccttc gccacccagg cccgcaactc gtccttcagt
     5821 tccttcgtcg gcgtgttgcc gccttccagc gtaacgaagg ccgaaatcgc ctggcccttc
     5881 agctcgtcgg gacgccccac tacggcggcc tcggccactt tcggatgcgc gaccagggct
     5941 gactccactt ccatcgttcc cagccggtgg ccgctgacgt tgatcacgtc gtcgacgcgc
     6001 cccatcagcc agaagtagcc gtcctcgtcc atgcgcgcgc cgtcgccggt gaagtagcag
     6061 cctggcacgt cggaccagta ctgcttgacg tagcggtccg gatcgcccca gatggttcgc
     6121 gccatcgacg gccatggctt gcggatgacc agcaacccgc cctgccctgc cggaacaggt
     6181 tggccttctt ttgtaacaac ctccggcaca attccgaaga aaggccgtgt tgctgaccca
     6241 ggcttggccg cgacggcccc cggaatcggc gaaatcatga tgccgcccgt ctccgtctgc
     6301 caccaggtgt cgacaatcgg acaccgtccc ttgccgatga cttcgcgata ccagatccac
     6361 gcctcgggat ttatcggttc gcctaccgtc cccagcaggc gcagactgtc catcttgtgc
     6421 tttgcgggat attcatcacc ccacttgatg aacgcgcgaa tggccgtcgg cgccgtgtag
     6481 aacaccgtga ctttgtggtc gtcgatgatc ttccaaaagc ggtccagggt cggatgattc
     6541 ggcgcgccct cgtacatcac ggtcgtcgta ccgttctgca ggatgccgta gaccacgtag
     6601 gagtgccccg tgacccagcc gatgtcggct gtgcaccagt aaacatcgtc ctcgcgcagg
     6661 tcgaagatgt acttgctggt cagatacgtc tggacggagt aaccgccggt ggtgtgcacc
     6721 aggcccttcg gcttgcccgt cgtgccagag gtatacagga tgtagagcgg atcttcagag
     6781 tccagttcct ccgccggaca ctctttctct gccgccttca tcagctcgtg ccaccagtgg
     6841 tcgcgtccgg cctccatgtt caccggcgat ccggtgcgct tgtagaccac gacattcttc
     6901 acggtggggc actgcccaac ggcttcgtct accgtcgctt tgagcttcac ttcgttgccg
     6961 cgacgatacg aagtgtcctg ggtcaggatc gcgacgcact gcgcatcgtt cacgcggtcc
     7021 acaatcgcgt tggccgcaaa gccgccaaaa atcaccgagt gcacggctcc gatgcgcgcg
     7081 cacgccagaa ttgcgatcgc cagctccggc gtcatcccca tatagacggc gacgcgatcg
     7141 ccttttttga tccccaggcc tttgagcaca ttcgcgaact tctgcacctc gctgtgcaga
     7201 tcggcaaagg tcagtttgcg cacctctccc ggctcgccct cccacaggat cgcaaccttg
     7261 tcggcgttgc cgttcacgac gtgccgatcg acgcagttgt agcagaggtt gattttgccg
     7321 cccacgaacc acttcgccca gggcagattc caatccagaa ccttctccca gggcttgaac
     7381 cagtgcagtt ccttcgcggc atcggcccag aacccctccg gatcccccac agaccgctga
     7441 tacatgcgct ggtattcggc cagatttttg acgtgcgctt gttttgcgaa ctcttcgggt
     7501 gggggaaaaa cccggtgctc ccgcagaatc gtttccagat tggggttgat cgcgatctcg
     7561 gatcccatgc actctccgtc agaccaaact aacgtcccgg cggtcaaagg cgcaaccgcc
     7621 tgaacggggc agaggtcccg gctacactat ccctcggagg tttccccttc ttgccctcga
     7681 acctcggcca cgtcctcgcc gtcattcccg cgcgccttgg gtccacgcgc ctgccccgca
     7741 aggtgctgcg cgagattgcc ggggagccca tgatcgcgtg ggtctaccgc gccgcgcgcg
     7801 cctgcccgct gctcgaccgg gtcctcatcg cgacagactc cgacgaggtc ctgcagttcg
     7861 cccagcagca cctactcccg gccgtcttca cgcctgtgga ctgcgccagc ggcaccgacc
     7921 gggtctgggc ggtcgcgcaa tccatccccg ccgacatcta cgtcaacatc cagggtgatg
     7981 agccgctcct cacccccgag cacttcacgc cgttgctgca gctcttcgag cggcctgagg
     8041 tccaggtcgc gacgctcgcc gtccgctgcc cacagcatga tgtgaacaat ccgagcgcgg
     8101 tcaaggttgt gaccgccgcc gatggccgag cgctttattt ctcgcgtgcc acgattccgt
     8161 tcgaccgcga caactccggc ttcgccggct atcgcaagca tctcggcgtc tacgcgtatc
     8221 gtaaagctgc gctgcagaaa ttcgcgaaca cgccgccttc gtggatcgag cagatcgagc
     8281 ggctggagca gcttcggctg ctcgacaacg gcatcgacat ccacgtcgcg cccgcgccca
     8341 gcgacacgat cggcgtcgac acggaagagg atctgcgcgc cgcggaggct gtcatcctga
     8401 gcgaaggtcg cccaaggcga ccgtagtcga aggacctgct cttgttctct ctcaagtagc
     8461 gcgcagatcc acgccccgcg actcccgccc gaacgccgcc accagcgcca catagcaggc
     8521 cacaatcgcc accgtcaccg ccagcaccgg tcccaatcca cccaaccgct ccgccgccct
     8581 ggtctgaatc accgcattcc acgacgccag caaattgccc agttgatacg cgaggccggg
     8641 aaacgtcgcg cgcaccgggc ctggcgacaa ctcgctgagc catgcgggca ccacgcccca
     8701 cgctccctgc accatgaact gcatcacgaa cgcgcctgcc gccaacatcc acacggagtg
     8761 cgacagcgcg tacagcggaa tggccggcag cgccagcagc gccgccagga taatcgctct
     8821 tttccgtcca accttctccg agaaagtgcc gaacaccatg ccgccggcca gcgcgccaat
     8881 gttgtagaga atcgcaatgg cgcccgtcag ctgggccgag aacccatgct ccttttgtaa
     8941 gaacgtggga tacaaatcct gcgtgccgtg ggaaaacgcg ttgaagccgg tcatcagcag
     9001 caccagaaac acaaacgtgc ccgcatactg cagcagcccc ttccgcagtc ccatcacagg
     9061 gattgtccgc tcctccagct cgccgcgctc cctccacgtc ggcgactcct ccaccttgcc
     9121 gccgatgtag aacaccagca gcgccggagc cgcgcccaca aggaacatcc cccgccatcc
     9181 aaatgcgtgg ccgtgccagg tgaaatgcga gaaccacggg aataacaacg cataggcggc
     9241 ggccgccacc aggtagccaa ccgcatatcc ttcctgcagc agcccggaaa agaatccgcg
     9301 attctctttc ggcagcgtct cgaaggcgag tgccgcgccc accccccatt cgccgcccat
     9361 tgcgatgccg aacagggcgc gcaggatgaa cagctcgtgc atcgatcccg taaaggcgca
     9421 cgccagttcc agcagcgagt agctcgcaat gttgaacatc agcgtgggcc gccgcccgaa
     9481 gcgatccgcg agcacgccga acacgaacgc ccccacgggg cggaacatca gcgtgatcga
     9541 aatcgccgca gcgacatcgg ttcgtgccac gcg
//
LOCUS       DASZYN010000105         2294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_516444, whole genome shotgun sequence.
ACCESSION   DASZYN010000105 DASZYN010000000
VERSION     DASZYN010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516444"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VHE33_02750"
     CDS             complement(<1..802)
                     /locus_tag="VHE33_02750"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HVT96396.1"
                     /translation="MISGRQDKTRGTTPGRGVAFGWVAWLAVLCLVWVSVPAHARGLG
                     QGQHGQRSQSARPAPRQPERRAAEPSRGSDAGRAAGNPGEARPDGNRPGAGSEERLSP
                     AVNRMEMRPAGNGGQGTYGDRDRPGHLGQWLMNHQNLSPQQREEQLRREPGFNRLSPD
                     QQQRVLNRLRSLDARPPEQRQRMIRRNEMFEQLNPEQKQGVRQSFQAWNQMTPDRHRM
                     LGRAFADLRQIPPDQRSAILNSARFSHEFSPDERRVLGNLLSIEPYEPR"
     gene            complement(931..1425)
                     /locus_tag="VHE33_02755"
     CDS             complement(931..1425)
                     /locus_tag="VHE33_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96397.1"
                     /translation="MICREVKESLPDLLLAPKQASAEVRAHVDGCAECARELKELEAT
                     LSLMDAWDAPEVSPYFDTRMAVLLREEQQSAPAGWLERMRARLTFGNRVNLRPLMAGA
                     MALVVAAGVGTYVGFGGFSPTKPPVQQVQSPVIKDLQSMDDNAQMFQQLSTLDQQDDN
                     SGNL"
     gene            complement(1464..>2294)
                     /locus_tag="VHE33_02760"
     CDS             complement(1464..>2294)
                     /locus_tag="VHE33_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HVT96398.1"
                     /translation="EGVLGPVPDVTEGWEGDDAGETGQSADSTDEEEEISAQPDDPNG
                     VSRDMASVALTTPGLGQRRAEEAQRLESGLKPLSGPVDWRSMSDAEIMVRVGAGEDAG
                     FNFLIEKYRKPIVNFMYRMVHNQAVAEELAQEVFLRVYRSRQTYRAEAKFTTWLYRIA
                     TNLGVNHARDTKHERAAQTIYLDQPDAETGTTPDVADSRPVVEEELLKDERMRAIRKH
                     VMALPERQRTAVLMHKYQGLDYKEIGAVLRLSESATKSLLFRAYQTLREKLKGFVEQG
                     "
BASE COUNT          376 a          899 c          571 g          448 t
ORIGIN      
        1 atctcggctc gtacggctcg atcgacagca aattccccag cacccgccgt tcatccggcg
       61 aaaactcatg cgagaaccgc gccgagttca ggatcgccga ccgctgatcc ggcggaatct
      121 gccgcagatc cgcaaatgcc cgccccagca tgcggtgccg gtccggcgtc atctggttcc
      181 acgcctggaa cgattgccgt acaccctgct tttgctccgg attcagctgc tcaaacatct
      241 cgttgcgccg aatcatcctc tgccgctgct caggtggacg cgcatcgagc gaacgcagcc
      301 ggttcagcac tctctgctgc tgatccggac tcagccgatt gaatcccggc tcccgtcgca
      361 actgctcttc tcgctgctgt ggcgacaggt tctgatggtt catcagccac tgccccagat
      421 gcccaggccg atcccgatcc ccgtacgtgc cctgcccgcc gttccccgcc ggccgcatct
      481 ccatccggtt caccgccggc gacagccgct cttccgagcc ggcgcctggc cggtttccgt
      541 caggccgcgc ctcgcccgga ttccccgccg cccgccctgc atcgcttcct cgcgagggtt
      601 ccgcagcccg tcgttccggc tgtcgcggcg ccggtctcgc cgattggctc cgctgcccgt
      661 gctgcccctg ccccagcccc ctcgcgtgcg ccggcacact cacccacacc agacacaaca
      721 cggccagcca cgccacccat ccgaacgcaa ccccgcgtcc gggcgtcgtg ccccgcgtct
      781 tgtcctgtct tccggagatc atcgccgcac ttactttccc agggccaaac tgcaaacgct
      841 tcaaagaaac cttccgggcc ggccttatcg ctgctccctc atcgcctcgc cctcgtcctt
      901 ccagcctcct gcttcttcct ccggctgcct ttacaggttc ccgctgttat cgtcctgctg
      961 atccagcgtg ctcagttgct ggaacatctg agcgttgtca tccatcgact gcagatcctt
     1021 aatcaccggc gactgcacct gctgcacggg aggcttcgtc ggcgagaacc cgccgaaccc
     1081 cacatacgtc cccacgcccg ccgccaccac cagcgccatc gcgccggcca tcagcggccg
     1141 caggttcaca cgattcccaa acgtcagccg cgcccgcatc cgctccagcc atccggccgg
     1201 agccgactgt tgctcctcgc gcagcagcac cgccatccgc gtatcgaagt acggcgaaac
     1261 ttcaggagca tcccacgcgt ccatcaaaga cagagtcgct tcgagctcct tcagctccct
     1321 ggcgcactcg gcgcagccgt ccacatgcgc ccgtacctcc gccgacgcct gcttcggggc
     1381 aagaagcaga tccggcaaac tctctttcac ttcacggcag atcattacca tcccccaaac
     1441 tcaaattctt cagcccaaca tcctcagccc tgctccacga aacccttcaa cttctcccgc
     1501 agcgtctgat acgcccggaa cagcaacgac ttcgtcgccg actcgctcaa ccgcagcacg
     1561 gcgccaatct ctttgtaatc cagcccctga tatttgtgca tcagcacggc tgtccgctgc
     1621 cgctccggca gcgccatcac atgtttgcgg atggcgcgca tccgctcgtc cttcaacaac
     1681 tcctcttcga ccaccggccg ggaatcggcc acatccggcg tcgtcccggt ctccgcatcc
     1741 ggctggtcga ggtagatcgt ctgtgcggcc cgctcgtgct tcgtatcgcg cgcgtggttc
     1801 acgcccaggt tcgttgcgat ccggtacagc caggtcgtga acttcgcctc cgcccggtac
     1861 gtctgccgcg agcggtacac acgcagaaac acttcctgcg ccagctcctc cgccacggcc
     1921 tgattatgca ccatgcggta catgaagtta acaatcggct tgcggtactt ttcgatcagg
     1981 aagttgaagc cggcatcctc gcctgcccca acgcgaacca tgatctcggc gtcggacatc
     2041 gaccgccagt cgaccggtcc cgacaaaggt ttcagaccgg actcgagcct ctgggcctct
     2101 tccgccctac gctgacccaa ccccggcgtg gtgagagcaa cgcttgccat atccctgcta
     2161 accccattcg ggtcgtcagg ttgcgcggaa atttcttcct cttcatctgt ggaatctgcg
     2221 gattggccgg tttccccggc atcgtcgcct tcccagcctt cggtcacgtc cggaacgggg
     2281 ccgagcaccc cctc
//
LOCUS       DASZYN010000106         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_840278, whole genome shotgun sequence.
ACCESSION   DASZYN010000106 DASZYN010000000
VERSION     DASZYN010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840278"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VHE33_02765"
     CDS             complement(<1..393)
                     /locus_tag="VHE33_02765"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HVT96399.1"
                     /translation="MKSFGNFWRKLKLLFGRETYAGELEEEMAFHREQMAKDLQESGM
                     TPEAARFAAMRQFGNTTQMRERSHEVVGFRAETIAQDVRFALRQLRKSPGFAVTAILM
                     LALGIGASTAIFGFVDAALIQPLPYAQQN"
     gene            complement(390..752)
                     /locus_tag="VHE33_02770"
     CDS             complement(390..752)
                     /locus_tag="VHE33_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVT96400.1"
                     /translation="MDYGLAVMAEKPELMPGTLDLLILQTLKRAPLHGYGIAQSIKRT
                     SDDVLTVEEGSLYPALQRLLLQGWVRAEWKITETNRRARYYTLTAAGRKQLGIELSKF
                     ERMVEAMGRVLRDAEEAG"
     gene            complement(1000..>2586)
                     /locus_tag="VHE33_02775"
     CDS             complement(1000..>2586)
                     /locus_tag="VHE33_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVT96401.1"
                     /translation="LWSAVGMILLIACVNLSNLLLARAAARGKEFAMRSALGASRGRM
                     VRQLMTESLILSAGGAVVGFGLAWGLVLWLAHQGSLALPLLSTLHIDGQALGWTVLIA
                     VGAAILFGLMPGLRMAAGNLIESLKDSGHGSSAGRKHEAVRAALVIAEVALACVLLVG
                     AGLLLRSFVKVMDIDLGFQPDQAASIRVEYDDSAPSDEASALKRGEIFQQVIARVSAL
                     PGVAAAGIVDYLPLGANRAWGAPVPQGKTFPEGALPGPLVYVCTPGYVRAMGMRLHGR
                     DFAWSDGPKSQEVVMINSAAARVYWPGEDAVGKVLVSGNEQLHVIGVVDDVHASSVEA
                     EKGWQIYYSAMQKGPSDAQLVIRTRVAPASLAGSVLRTLRELNPNQPAVEFQPIRAIV
                     DRAVSPRRFFMLLVGIFAGLGLLLASLGIYGVIAYGVTRQTQEIGIRMALGATRGRVQ
                     RTVLGRTLGLVLLGIVVGTAVSYAAARMIASLLFGTEPTDPVTYLGMILLLIGVALLA
                     GYLPARRASRIDPLVALRTN"
BASE COUNT          491 a         1006 c          652 g          437 t
ORIGIN      
        1 attctgctgc gcatacggca gcggctggat cagcgccgcg tccacaaagc caaatatggc
       61 cgtgctcgcg ccaataccca gcgccagcat cagaatcgcc gtcacagcga accccggact
      121 tttgcgcagt tgccgcaacg cgaatcgcac atcctgtgca atcgtctccg cgcggaaacc
      181 caccacctca tggctccgct cccgcatctg cgtcgtatta ccaaactgcc gcatcgccgc
      241 gaagcgcgct gcctccggcg tcattccgct ctcctgcaga tccttcgcca tctgctcgcg
      301 atggaatgcc atctcttcct caagttcccc ggcgtatgtc tcccggccga agagcagctt
      361 caatttccgc cagaaatttc cgaagctttt catcccgcct cctcggcgtc gcgcagcacg
      421 cgccccatcg cctccaccat gcgttcgaat ttcgacaact cgatgcccag ttgcttccgc
      481 cccgccgcgg tcagcgtgta ataacgcgcg cgccggttcg tctccgtgat cttccattcc
      541 gctctgaccc acccctgcag aagcagccgc tgcagcgcgg gataaagcga tccttcctcc
      601 acggtcagaa cgtcatcgga ggtgcgtttg attgactgag cgataccgta cccgtgcagc
      661 ggcgcgcgtt tcagcgtctg caggatcagc aggtccagcg ttccaggcat cagttccggc
      721 ttctcggcca tgacggctaa accatagtcc atctatggat agagaatcaa cgcctatgtg
      781 acgaacgctc cggccgagcg tattgccttc cggcacggct acaggcgacc agctacacgc
      841 tgctctcctc gggcttcgac cggaggatct ggtccctccc gcaaagcggt gccgcgccag
      901 cggcacgacg gtagaagccc cggccttcag gccggggaaa aggatccgcg ggttcggagc
      961 ctttaggccc ggaacgtatt caatcacgcg gtcacggtac tagttggtcc gtagcgccac
     1021 cagcggatcg atccgcgacg cccgcctcgc cggcaaatat ccggccagca acgcaactcc
     1081 aatgagcagc aggatcattc cgaggtacgt caccggatcg gtcggctcgg ttccgaacag
     1141 cagcgatgca atcatgcgcg ccgccgcata ggagaccgcc gtgccaacta cgattcccag
     1201 cagcaccagc cccagcgttc tgcccagcac cgtccgctgc acgcgccccc gcgtcgcccc
     1261 cagcgccatg cggatgccga tctcctgcgt ctgccgcgtc accccgtacg caatcacgcc
     1321 gtagatgccc agcgacgcca gcagcagccc cagccccgcg aaaattccca ccaacagcat
     1381 gaagaagcgc cgcggcgaca ccgcccggtc cacgatcgct cgaatcggct gaaactctac
     1441 cgcaggctgg ttcggattca gctcccgcag cgttcgcagc acgctccccg ccagagacgc
     1501 cggcgccacg cgcgtgcgaa tcaccagttg cgcatcgctc ggccctttct gcatcgctga
     1561 gtagtagatc tgccaccctt tttccgcttc cacactcgaa gcatgcacat cgtcgacgac
     1621 gccgatcacg tgcagttgtt cgttgccgct caccagcacc ttgcccaccg catcctcgcc
     1681 aggccagtac acacgcgccg ccgcggagtt gatcatcacc acctcctggc tcttcggccc
     1741 atcgctccat gcaaagtcgc gcccgtgcaa acgcatgccc atcgcccgca cgtagcccgg
     1801 cgtgcacaca tacaccagcg gacccggcag cgcaccctcc ggaaacgtct tgccctgcgg
     1861 caccggcgct ccccatgcgc gattcgctcc cagcggcaga tagtctacga tccccgccgc
     1921 cgctactccc ggcaacgcgc tcacccgcgc gattacctgc tgaaaaattt ctccgcgctt
     1981 cagtgcgctc gcctcgtcgc ttggcgcgct gtcgtcatac tccactcgaa tcgacgccgc
     2041 ctgatccggc tggaatccca gatcgatatc catcaccttc acgaaactcc gcagcaacag
     2101 acccgcgccc accaacagta cgcacgccag agcaacctcg gcaatcacca gcgccgcccg
     2161 caccgcctca tgcttccgcc ccgcgctcga tccgtggccg gagtctttca gcgattcgat
     2221 cagattcccc gctgccatgc gcaagcccgg catcagtccg aataaaattg ctgccccaac
     2281 agcgatgagc accgtccacc ccagcgcctg cccatcgata tgcagcgtgc tcagcagcgg
     2341 cagcgccagt gatccctgat gcgccagcca cagcacaagc ccccatgcca gcccgaatcc
     2401 caccaccgcg ccacccgccg aaagaatcag gctctccgtc atcagctgcc gcaccatgcg
     2461 ccctcggctc gcgcccagcg cgctgcgcat cgcgaattcc ttcccgcgcg ccgccgcgcg
     2521 cgccagcagc aggttcgaca ggttcacgca cgcgatcagc aaaatcattc ccaccgccga
     2581 ccacag
//
LOCUS       DASZYN010000107         1564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_580614, whole genome shotgun sequence.
ACCESSION   DASZYN010000107 DASZYN010000000
VERSION     DASZYN010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580614"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="VHE33_02780"
     CDS             <1..1179
                     /locus_tag="VHE33_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT96402.1"
                     /translation="VFQHNRFREGYFKAILIADRGRSEWKAGLESDNTFLHENTSYAI
                     TDPNQYDDGTPLNFAFSGQRPDLEQSAFVEDRLRLGNWSMSAGLRWDHYQLLVNKQAL
                     EPRLAVSRYFPSTQSILHFSWDRIFQTPSFENILLSSSTQIESLDPTTFLRLPVEPSV
                     GDYFEGGLTQGFWDKARLRVNYFRRVMNDFADDDQIDNTTVSFPISFRKAIVYGIEGK
                     LDVPDWRRISGFVSYSWQVGNAWNPVTGGLFLGADAAAAESRQSGHFPISQDQRNTVR
                     GRLRYQIHPRFWLAGGIQYDSGLPFEFDGDPSTVLAQYGQAVLDRINFDRGRADPSFE
                     VSASAGVDLYQSERLHMQLQADGENLNDTLDVIDFGGLFSGNAIGPPRSGMVRLTTTF
                     "
     gene            1269..>1564
                     /locus_tag="VHE33_02785"
     CDS             1269..>1564
                     /locus_tag="VHE33_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96403.1"
                     /translation="MSQHLLTRRSFLASAAMTAAAARLGAQTKTDAVTLEIPIEADGP
                     HMPSDFVGLSYEVQQLADPSFFSARNTGLVRAFRELTPQGVLRLGGNTSEFAWW"
BASE COUNT          317 a          482 c          427 g          338 t
ORIGIN      
        1 gtcttccagc acaaccgctt ccgcgagggc tacttcaaag cgattctgat cgcggatcgt
       61 ggccgaagcg agtggaaagc aggactcgag tccgacaaca cattcctgca tgagaacacg
      121 agctatgcca ttaccgaccc gaaccagtac gacgatggca caccgctcaa ctttgctttt
      181 tcggggcaac ggcccgatct ggaacaatcc gcctttgtcg aggatcggct ccgccttgga
      241 aactggtcga tgagcgcggg cctgcgctgg gatcactatc aacttctcgt caacaagcag
      301 gcactcgagc cgcggttggc ggtctcacgc tatttcccct ccacgcaaag tatcctgcat
      361 ttttcctggg accgcatctt tcagacgcca tcgttcgaaa acattttgct ttccagctcc
      421 actcaaattg agtcgctcga tcccacaaca tttctccggt tgcccgtcga gccatcggtg
      481 ggcgactact tcgaaggtgg tctgacgcag ggtttttggg acaaggcgcg cctccgcgtc
      541 aactactttc gccgcgtgat gaacgatttc gccgatgacg atcagatcga caatacgacc
      601 gtcagctttc ccatcagttt tcgaaaggcc atcgtctacg gcatcgaggg caaactggat
      661 gttccggact ggcgaagaat ctctggattc gtcagctact cctggcaggt cggcaatgcg
      721 tggaacccgg tgacaggggg ccttttcctg ggggctgatg cggccgccgc agagtcccgg
      781 cagtccggcc actttccgat ttcgcaggat cagcgcaata ccgtacgcgg acgcctccgc
      841 taccagatcc atccccgatt ctggctcgcc ggcggaatcc agtacgattc cggccttccc
      901 tttgagttcg acggcgaccc ctccacggtt ctcgcccagt acggacaagc ggtgctggat
      961 cgcattaact tcgatcgggg gcgcgccgat cccagctttg aggtgagcgc ctctgccgga
     1021 gtggatcttt accaatccga gcggcttcat atgcagctcc aggcagacgg agaaaacctc
     1081 aatgacactc tcgatgtcat cgattttggt gggctgttct ccggcaacgc catcggaccg
     1141 ccacgcagcg ggatggttcg gctcacgaca acattctgac ggcaatcaat gaatagctgc
     1201 cgcttgccga gcgcaggtca tagaatactc agcgaacgac ttcacagaat tcgccgagga
     1261 ttccatcgat gtctcaacat ctgttgacga ggcgcagttt tctcgcctcc gctgcaatga
     1321 cggccgccgc cgcgcgtctg ggagcacaaa ccaaaaccga tgccgtcacg ctcgagatcc
     1381 cgatcgaggc agacggtcca cacatgccgt cggatttcgt cggcctttcc tacgaagtgc
     1441 agcagttggc cgatcccagt ttcttttcgg ccaggaatac cggtttggtt cgcgctttcc
     1501 gggaactgac ccctcaggga gttctgcgcc tgggaggcaa cacgagtgag ttcgcctggt
     1561 ggaa
//
LOCUS       DASZYN010000108         2315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_645027, whole genome shotgun sequence.
ACCESSION   DASZYN010000108 DASZYN010000000
VERSION     DASZYN010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645027"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VHE33_02790"
     CDS             <1..280
                     /locus_tag="VHE33_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96404.1"
                     /translation="PNVSHVFHKGSSIRITLDAPSPTGDWAFDAVPTGVATVNSNRVH
                     PSSLVLGVMRTGIRTGRLTACSQMPGTPCRKNTVAVPHRGELRLTAPH"
     gene            852..1376
                     /locus_tag="VHE33_02795"
     CDS             852..1376
                     /locus_tag="VHE33_02795"
                     /inference="COORDINATES: protein motif:HMM:NF020906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 domain-containing protein"
                     /protein_id="HVT96405.1"
                     /translation="MTDNRAVPSRELSYDPVGATDPASAAWQPGRHRFQATVVVGRGE
                     ECWTASREAVLAWAVKVRSGFLVTGGPQTVRPGQDYELTFRIGPIAIREPVRVVAVMD
                     EPDRCGFAYGTRPGHPVAGEEAFIVHRDGRGVVRLTLRSLTMPASGWRALAFPVFVVA
                     QRAFRHRYLRALNS"
BASE COUNT          379 a          742 c          759 g          435 t
ORIGIN      
        1 tcccaacgtc agccacgtct ttcacaaggg ctcgtcgatt cgcatcaccc tcgacgcacc
       61 gagcccgaca ggtgactggg ccttcgacgc cgtaccgacc ggagtcgcca cggtcaacag
      121 caatcgcgtc cacccatcgt cgctggtcct cggcgtgatg cgcaccggca tccggacggg
      181 gcggctgacg gcgtgctcgc agatgccggg tacaccgtgt cgcaagaaca ccgtggccgt
      241 tccgcaccga ggcgagctgc gcttgaccgc gccgcactag gcaatcacaa ccggctggct
      301 tggcgagttc tcgaggagtt ccttgttgcc gccgacgacg cggtcgccgc gcttgacgtc
      361 ggtctcgctg gaggaacccc tttgccagcc gcacctatgt cacatgcgtg ttcctatagc
      421 gacaccgatg tgacacaggt gcagcagaag tgccgctctg cgtcacatgg cccatcgaaa
      481 ccgccaggca ccaatccttc gcgcgctggc ataagcgccc tgtccggcac accgaatctt
      541 ggggcgaggg ctaccttcga tcgcgatgtc cgagtctgat cgccgcgggg tcgattacgc
      601 ccgggcggag aggctgcgcc gcggcaggcg cacggtgctc tccctgatgg gcctacccgg
      661 cctcctgatg ctggcggccg gcatcgcctt ggagttcgcg catctcaagg gaaatgtgac
      721 agcagtcccg tttgtgatga tcgtgtgggg tgcgggcatt ctgctgagct gcgcttgggg
      781 acttcagcga aacatcaaga aacagcgaga acttcggcac tcgccagggg cataggtgcc
      841 ggtatcggtc ggtgaccgac aatcgtgcgg tgccttcgcg agagctcagc tacgacccgg
      901 tcggcgcgac cgatccagcg tcagcggcct ggcaacctgg ccgccatcgc tttcaggcga
      961 cggtcgtggt ggggcgcggc gaagaatgct ggacggcaag tcgagaggcg gtactcgcct
     1021 gggcggtgaa ggtccgcagc gggttcctcg tcaccggagg cccgcaaact gtcagacctg
     1081 gccaggacta tgaactcacg ttccgcatcg gccccatcgc gattcgggag cccgtgagag
     1141 tggtagccgt catggatgag cctgaccgct gcggcttcgc gtacggaacc cgaccaggtc
     1201 atcctgttgc gggtgaggaa gccttcatcg tccaccgcga tggccgcgga gtggtccggc
     1261 tgacgctgcg atcgctaacg atgcccgcaa gcggttggcg agcgctcgcc tttccagtgt
     1321 ttgtcgtggc gcagcgcgcg tttcgtcaca ggtatctgag ggctctcaac agctgagcgc
     1381 gccgcgctgt atgtgccgct agggaacatt cgccttccgt gccccgcggc gccgttgcca
     1441 ctcggtcgct atgaagaatc caaccccggc gaacaggctg gttgcgagcg aggcaccgag
     1501 tgacgacccg atcgctaggt tgaggccaag aacaatcagc atcaacagca agcttgcgat
     1561 caaagcaccg cgcactcctt gatcgtcgca gatgtggcgc aggatttgcg ccgtcgaata
     1621 ggtgccgttc tagctcgacg gaaccccaga tgtggtcgag acctacgtag gaggcgcaac
     1681 tccggggcta gttctgcctc ctacgtcggt ctcggcgctg cgaaccgagg tgccgctgta
     1741 gcgcaggttg ggcatcgtcc cggttgctca acaacggctc gcgttgtgtc gacactggaa
     1801 cggtgccatt tgatgcgtgt gcatcgtgcg gtgcaccgtc gcaaggtgcc tatcgctgcg
     1861 ctcgctacaa ccccatcgga gcgaggccag ggtggaggag ccacacctgg ccgaagcgtc
     1921 gatccggcag acctagaagt tcgcagcagg ttctcgccgt ggttcttccc gatggctcgc
     1981 cctgtcacga ccctggtgtg ggcgcagccg cggaaccgtt ggtttcgctg ggcagtcaac
     2041 gccgtgcgga tagcgctcgt ggtcttcctc gcgggcgtgc tggtgttcct agtcatcgcg
     2101 gcctgggggt agtcacctcg cacccggccc tgcgtcctgg aagtgccgat agcgtccgac
     2161 gctgggcgga cgcgaagtgc cgctcggacg cgagccccgg agcgatgcgt tgctagtccg
     2221 gcggtctttg ggcgagcgcc tcgcgttgcc gcttaatggc gtacgcctcg tctctcgact
     2281 ccgcgatgaa cgcctgttcc tgggcattct tcgtc
//
LOCUS       DASZYN010000109         2754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_516513, whole genome shotgun sequence.
ACCESSION   DASZYN010000109 DASZYN010000000
VERSION     DASZYN010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516513"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..2544
                     /locus_tag="VHE33_02800"
     CDS             127..2544
                     /locus_tag="VHE33_02800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT96406.1"
                     /translation="MFADVKYAVRQLKKSPGFTVTAILTLALGIGANAVVFSVLNAVV
                     LHPLNVPNAGNLYMVQRFRFPSQSVPDYRDLRDRNHSFESMFSYNIMGPAGLETGGNP
                     ITAWPYEVSGNYFDALGIHPYIGSFFHGSDDRAANSAPWIVLSYALWHTQFHDDRGIV
                     GRSLQVNKHPYTVLGVTPPEFRGTELFFAPAFFVPIVDQPQMEGYNSLEQRGNHSSMV
                     VGRLKPGVTPEQATADLNVIGAWLSKTYPHDDDGVNFTLARPGLVGDALGAPARAFMT
                     GLMLLAGLILLAACANLGSLFAARTADRGKEIAVRMALGSKRRLILRGLITEAVLVSL
                     VGGVVGLAGGIAILRWLSAWQPIPNIPINVPVNPDLWTYLVSFALAVLSGVLFGLVPV
                     RQVLKSDPWQIIRAGGSVMSHLRRFTLRDVLLVAQIAICAVLVTSSLVAVRGLVRSLH
                     SNFGFEPANVVLAGTDLKWAGYTGDNVQIMQRKIVDALHAIPGVTAAGYSSGLPLSLG
                     SGDSFVYTDATTDFRPTNYAADAMQYRVSPGYFAATGTRMLAGREISWHDDSKAPKVA
                     VVNEEFARKVFGSVNKAVGGYFKFWGSERAQVVGVVENGKYRSLTEDQQPAMFNSIQQ
                     YQASGTYFLVRSRRATNEIAGAFQRTMHALDPSMSVTIETWNQELSSALFPSRVASVA
                     LGVLGLLGAMLAVTGIFGMASYVVAKRMRELGIRVALGARQQEVLGAALGRAFALLAA
                     GSLAGIGLGVLATKVLSSIVYQATPKDPLVLGGVVLAMMVLGLVASWIPAQKALAVDP
                     MILLREE"
BASE COUNT          487 a          698 c          998 g          571 t
ORIGIN      
        1 ctgttgctgc ctccaaagtg cccgacacca gttaccgaag gcaggggtag ggcgaagaaa
       61 tagcgataca gatcaggaac accggcgtag ggtggagcgt actgcctggc agcacggagg
      121 cgaggcatgt tcgcggatgt gaagtacgcg gtgcggcaac tgaagaagtc gccaggattc
      181 acggtgacgg cgattctgac cctggccctg ggaattggcg cgaacgcggt ggtattcagt
      241 gtgctgaatg ccgtcgtgct gcatccgctg aacgtgccaa acgccgggaa tctgtacatg
      301 gtgcagaggt tcaggtttcc ttcgcagtcg gttcccgatt atcgggatct gcgcgaccgg
      361 aaccacagct tcgagtcgat gttctcctac aacatcatgg ggccggcggg gctggagacc
      421 ggcggcaatc cgatcaccgc gtggccgtat gaggtcagcg ggaactactt cgatgcgctg
      481 gggattcatc cttacatcgg gagtttcttt catgggtcgg atgatcgcgc cgcaaacagc
      541 gcaccctgga tcgtgctgag ctacgcgctg tggcacacgc agttccacga cgatcgcggg
      601 attgtcgggc gcagcctgca ggtgaacaaa catccgtaca cggtcctggg tgtgacgccg
      661 ccggagtttc gcgggactga actgtttttt gcgcctgcgt tcttcgttcc gatcgtggac
      721 cagccgcaga tggagggtta caacagcctc gagcagcgcg gaaaccactc ttcgatggtg
      781 gtgggtcggt tgaagcctgg agtaacgccg gagcaggcca ctgcggatct gaatgtgatt
      841 ggagcgtggc tgtcgaagac ctatccgcac gacgatgatg gggtgaattt caccctggca
      901 cggccggggc tggtgggcga tgcgctgggc gctcccgcgc gcgcgttcat gacaggcttg
      961 atgttgctgg ccgggctgat cctgctggct gcctgtgcga acctgggaag cttgtttgcg
     1021 gcgcggaccg ccgatagggg aaaagagatc gcagtgcgca tggcgctggg atcgaagcgg
     1081 cgtctgattc ttcgcgggct gattacggaa gccgttctgg tatcgctggt gggaggagtg
     1141 gtggggctgg cgggcgggat tgccattctg cgctggcttt ccgcatggca gccgataccg
     1201 aacatcccta ttaatgtgcc ggtcaatcca gatctgtgga cgtatctggt gtcgtttgca
     1261 cttgctgtgc tgagcggagt gttgtttgga ctggtgccgg tgcggcaggt gctgaaatcc
     1321 gacccgtggc agatcattcg ggccggcggc agcgtgatga gccatctgcg gcgcttcacg
     1381 ctgcgtgatg tgctgctggt ggcgcagatt gcgatctgcg cggtgctggt gacgtcgtcg
     1441 ctggtggcgg tgcggggact ggtgcggtcg ctgcacagca attttggttt tgagccggcc
     1501 aacgtagtgc tggcgggcac cgatctgaaa tgggccggat atacagggga caacgttcag
     1561 atcatgcagc gaaagatcgt cgatgcgctg catgcgattc cgggggtcac cgcggctggg
     1621 tactcgagcg ggctgccgct gagcctgggc agcggcgatt cctttgtcta cacagacgcg
     1681 acgacggatt tccggccgac gaactatgcc gccgatgcga tgcagtacag ggtttctcct
     1741 ggatatttcg ccgcgacggg aacgaggatg cttgccgggc gtgagataag ctggcatgac
     1801 gacagcaagg caccgaaggt ggcggtggtc aacgaggaat tcgcgcgaaa agtgttcggg
     1861 tcggtgaaca aggcggttgg cgggtatttc aaattctggg gatcggagcg ggcccaggtg
     1921 gttggggtcg tcgagaacgg caaataccgg tcgctgacgg aggatcagca gccggcgatg
     1981 ttcaattcga ttcagcagta ccaggcgagc ggcacgtatt tcctggtgcg gtcgcgccgc
     2041 gcaacgaacg agattgcggg ggcgttccag cggacgatgc acgcactgga tccgtcgatg
     2101 tcggtgacga ttgaaacgtg gaaccaggag ctgagctcgg cgcttttccc gtctcgtgtg
     2161 gcgagcgtgg cgctgggggt gctgggattg ctgggcgcga tgctggcggt gacgggcatc
     2221 ttcggcatgg cctcgtatgt ggtggcgaag cggatgcggg aactgggtat tcgcgtggcg
     2281 ctgggggctc gccagcagga agttctggga gcggcgctgg gacgggcgtt cgcgctgctg
     2341 gccgccggct cgctggcggg aatcgggctg ggcgtgctgg cgacgaaagt gctgtcgtcg
     2401 atcgtgtatc aggctactcc gaaggatcct ctggtgctgg gcggagtggt cctggcgatg
     2461 atggtgctcg gactggtggc atcgtggatt ccggcgcaga aggcgctggc ggtggatcca
     2521 atgattctgc tgcgggaaga gtgaggccgc cgcaatagcc tgtagccagt agccaatagc
     2581 ctgtagccag tagccagtag ggaagagacc taaagcggag ccctttcgag tgatctaagt
     2641 cgttctgggc gcgctccctc aggatgcgcg actttttaat ggttgcgcgg gttatgaggg
     2701 ggaggacaga ctgtctctaa aggacatccc cagccatgcc cacgaatctt tctg
//
LOCUS       DASZYN010000110         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1097314, whole genome shotgun sequence.
ACCESSION   DASZYN010000110 DASZYN010000000
VERSION     DASZYN010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097314"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            489..1241
                     /locus_tag="VHE33_02805"
     CDS             489..1241
                     /locus_tag="VHE33_02805"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HVT96407.1"
                     /translation="MADALAPDTSVQPLNEMQRIVDTFAAPSKLGKDIVRSAAWWGPF
                     IILVIVSIGFSFTVQKKIGWAAVYDNAISQNPKMKQMIENLPADQQVVARQKGIARQP
                     YTAYAAPVFSLIFTAIFALLIWPTINFGFGGTAKFSKIFAVLMYSNIISYGVRFLLAV
                     IAIFAGLSPESFNFQNPVGTNLGYYLVGSAPLWLVTMGSFIDIFGIWSMVVSSIGCGI
                     VARVKTSSAAIAVFGWWLLFMLGITGLVAAFS"
     gene            complement(1313..>1592)
                     /locus_tag="VHE33_02810"
     CDS             complement(1313..>1592)
                     /locus_tag="VHE33_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96408.1"
                     /translation="IEAPQEFAGALMGDLNQRRGRVQGMDSHGANSIIRAEVPMAEML
                     TYGQTLTAMTQGRGSFHMEMNHYDIVPQLIADKIVANAKKPTGEEEEE"
BASE COUNT          304 a          429 c          487 g          372 t
ORIGIN      
        1 ccgcggccgc actggccaga accgtgtgag aggcatgccc cgcaacagtg ccgcagtgcg
       61 acgaggcact ccggtgacgg acgtggtgac ggtgaggcgt cggagcggtg gcaactgtcc
      121 tggctgccaa acaggggagc gccagcataa cgctggctgc gacggcgaag gggactggac
      181 tgcgcatgcg gacctgaggg agtgatcgaa agagcaatac cagagtatcg gacactggcg
      241 caggaaaaaa gtagcgatca gggtacgtgg cactggcaat cctcaacggc agttatccgc
      301 cagcgaatgg ttccgcgatg gagataaaag cggccttctg ctgtggttgc tcctgcgcgc
      361 tcagtgaacc ggagtggatt cccgcggagc cgggaatttc atttcgattc ggacaggccg
      421 gaggtacaat tcctcgaaat cagaaaaagc ggcggttcag tattctgaat ttttccggga
      481 gggatgttat ggccgatgct cttgcacctg acacgtctgt gcagccgttg aacgaaatgc
      541 agcgaatcgt ggatacgttc gccgcgccgt cgaagcttgg taaggacatc gtgcgcagcg
      601 ctgcgtggtg gggtcctttc atcattctgg tgattgtcag tattggattt tccttcacgg
      661 ttcagaaaaa gatcggatgg gctgccgtct atgacaacgc gatcagtcag aacccgaaga
      721 tgaaacagat gatcgagaat ctgcctgccg accagcaggt cgtggcgcgc cagaagggca
      781 ttgcccggca gccctacact gcctacgccg cgccggtctt ttcgcttatt tttacggcaa
      841 ttttcgcatt gctcatctgg ccgacgatca acttcggctt cggcggaacc gcaaaattct
      901 cgaagatatt tgccgtcctg atgtactcca acattattag ctatggcgtg agattcctgt
      961 tggccgtgat tgccatcttt gctggtctct cgccggaaag tttcaacttt cagaacccgg
     1021 taggtacgaa tcttggctat tacctggttg gcagcgcgcc cttgtggctg gttacgatgg
     1081 gatcgttcat cgatattttc ggaatttggt cgatggtcgt ctcctcgatc ggatgcggaa
     1141 ttgtcgcccg cgtgaaaacc agttccgccg caatcgctgt gtttggatgg tggctgctct
     1201 tcatgctggg aattaccgga ctcgttgcgg cgttcagcta gaggaagtta cgaatccttc
     1261 gcaagaggcc cgccgagctg gcgggccttg ttgtttgcgg ttggctggaa gcctactcct
     1321 cttcttcctc ccccgtcggc ttcttcgcgt tggcgacgat cttgtccgcg atcagttgcg
     1381 gcacgatgtc gtagtgattc atctccatgt ggaagctgcc acggccctgc gtcatcgccg
     1441 tgagggtctg gccgtaggtg agcatctccg ccatcggcac ttcggcgcgg atgatggagt
     1501 tagcgccgtg actgtccatg ccctggacgc ggccccggcg ctgattcagg tcgcccatca
     1561 gcgctccggc gaattcctgc ggagcctcga tt
//
LOCUS       DASZYN010000111         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_904280, whole genome shotgun sequence.
ACCESSION   DASZYN010000111 DASZYN010000000
VERSION     DASZYN010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904280"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            309..>2264
                     /locus_tag="VHE33_02815"
     CDS             309..>2264
                     /locus_tag="VHE33_02815"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HVT96409.1"
                     /translation="MSVNAGVWIFVAMSAVSLVAAYLFTGGLMRSSFISRRWARLAGV
                     MATLMMLGQGAALAQDSGGGEANLRLPDLSIVKFWGMNGHALLSIGLLFCAGGLLFGL
                     AIYVQLKNLPVHRTMREISEMIYETCKTYLSTQGKFILLLWAFIAVIIALYFGYLAPV
                     PGKSIGVTLPIILLFSLIGIAGSYGVAWFGIRVNTFANSRTAFAGLRGKPYPVYAIPL
                     KAGMSIGMLLISVELLIMLFILLFIPRDYAGPCFIGFAIGESLGAAALRIAGGIFTKI
                     ADIGADLMKIVFKIKEDDARNPGVIADCTGDNAGDSVGPSADGFETYGVTGVALITFI
                     LLAVKSPETQVQLLVWIFVMRVAMLVSSAGAYFLNSAIAHAKYANSDEMDFETPLTSL
                     VWITSIVSIILTYIVSYLMIPALGDGTLWWKLASIISCGTLAGAIIPELVKVFTSTKS
                     SHVKEVVTSAQEGGASLGILSGFVAGNFSAYWLGLCMVALMGLGYFFTLGLSMESLML
                     APAVFAFGLVAFGFLGMGPVTIAVDSYGPVTDNAQSVYELSLIESVPGIEAELKKDFN
                     IDVRFEEAKHLLEANDGAGNTFKASAKPVLIGTAVVGATTMIFSIIMALTGGLTRDVQ
                     NLSLLHAPFLLGLITGGAMIYWFTGAST"
BASE COUNT          387 a          652 c          694 g          531 t
ORIGIN      
        1 gcgaccgccg acattgagat gacccgcatt cgcggggtcc acggcccgcg ttttcttgac
       61 gtagccctgg tgcgctgagt cggggaacac acagggaggc cgtcgccgtt tggcgtattt
      121 ttcgcccgtt cttcgccgca gcacgacggt gcgaggatgg gccttacttt ggtgttaacg
      181 cctgtattgc ccatcacatg ccaaagcgtg aatcccctgt tatgctctct tgttgccgtg
      241 atacagttcc tgagcgcgtt cggggtatcg gacgcacaaa gcctaaaccg actttcatgg
      301 aggggtgtgt gtcagttaac gccggggtgt ggatttttgt tgcaatgagc gccgtgtcgc
      361 tcgtcgcggc ttatttgttc acgggaggat tgatgagaag cagcttcatt tcgcggcgct
      421 gggcgagact cgccggcgtg atggccacac tcatgatgct cggtcagggc gcagctttgg
      481 cacaggactc gggcggcggc gaagccaatc tccggctccc ggatttgtcg atcgtgaagt
      541 tctggggcat gaacgggcat gccctgctgt cgatcgggtt gctgttctgc gccggcggac
      601 tgttgttcgg cctggcgatc tacgtgcagt tgaagaacct gcccgttcat cgcaccatgc
      661 gcgaaatttc cgaaatgatc tatgagacct gcaagacgta tctgtcgacg cagggcaagt
      721 tcattctgtt gctgtgggcc ttcatcgcgg tgattatcgc gctgtatttc ggctatctgg
      781 cgccggtacc gggaaaatcg attggggtta cgctgccgat tattttgttg ttctcgctca
      841 tcggcatcgc gggcagctac ggcgtggcgt ggttcggcat ccgcgtgaac acctttgcga
      901 attcgcggac ggccttcgca ggcctgcgcg gcaagccgta tccggtttat gcgattccgc
      961 tgaaggccgg catgagcatc ggcatgctgc tcatctccgt cgaactgctg atcatgctct
     1021 tcatcctgct gttcatcccg cgggattatg caggcccgtg cttcatcggc ttcgccatcg
     1081 gcgagagcct gggggccgca gccctgcgta tcgccggcgg catcttcacc aagatcgccg
     1141 acatcggcgc cgacctgatg aagatcgtct tcaagatcaa ggaagacgat gcgcggaacc
     1201 cgggcgtgat cgccgactgt accggtgaca acgcgggcga ctcggttggg cccagcgcgg
     1261 acggctttga aacttacggc gtcaccggcg tggccctgat caccttcatc ctgctggcag
     1321 tgaagagccc cgaaacgcag gtccagctgc tggtctggat cttcgtgatg cgcgtggcga
     1381 tgctggtttc gagcgccggc gcgtatttcc tcaacagcgc catcgcccac gccaaatacg
     1441 cgaactccga cgaaatggac ttcgagacgc cactgacctc gctggtgtgg atcacgtcca
     1501 tcgtttccat catcctgacc tacatcgtct cgtacctgat gattcccgcg cttggcgacg
     1561 gaacgctgtg gtggaaactg gcttcgatca tctcctgcgg aactctggcg ggtgcgatca
     1621 tccccgaact ggtgaaggtg tttacttcga ccaagtcgag ccacgtgaag gaagtcgtca
     1681 cctccgcgca ggaaggcgga gcttcgttgg gaatcctgtc gggatttgtg gccggcaatt
     1741 tttccgccta ctggctgggt ctgtgcatgg tcgcgctcat ggggctgggc tatttcttca
     1801 ctctgggcct gtccatggag agcctgatgc tggctcccgc cgttttcgcg tttggcctgg
     1861 tcgcgttcgg cttcctgggc atgggtccgg tgacgattgc cgtggattcc tacggaccgg
     1921 tgacggacaa tgctcaatcg gtctacgaat tgtcgctgat cgaaagtgtt ccgggcatcg
     1981 aagctgagct gaagaaagac ttcaatatcg acgtccggtt cgaagaggcc aaacacctgc
     2041 tggaggccaa cgacggggcc ggcaatacgt ttaaggcttc agccaagccc gtgctgatcg
     2101 gcacggccgt ggtcggcgct accaccatga ttttctccat catcatggcg ctgaccggcg
     2161 gattgacccg ggatgtacag aacctttcct tgctgcacgc acccttcctg ctcggactga
     2221 tcacgggcgg agccatgatt tactggttca ccggcgcttc cacg
//
LOCUS       DASZYN010000112         8570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_324523, whole genome shotgun sequence.
ACCESSION   DASZYN010000112 DASZYN010000000
VERSION     DASZYN010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8570
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324523"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2879)
                     /gene="secA"
                     /locus_tag="VHE33_02820"
     CDS             complement(<1..2879)
                     /gene="secA"
                     /locus_tag="VHE33_02820"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HVT96410.1"
                     /translation="MIGTKVLNKVFGTSNERAVKRLLPRVAEINALEPQVQAMSDEQL
                     RAKTAEFRARIASALEGITDEEARVAAEKQALDDLLPEAFAVVREAGWRAVNMRHFDV
                     QLVGGIVLHQGKIAEMKTGEGKTLVATLPVYLNALAGHGVHVVTVNDYLAKRDAEWMG
                     KIYEFLGLTVGVIVHDLDDNQRRKAYGSDITYGTNNEFGFDYLRDNMKFELNDCVQRG
                     HFFSIVDEVDSILIDEARTPLIISGPTDQTTDKYARVNRIIPSLEMGEEIEKSPEEKI
                     LTGDYVVDEKHKTIAVTDEGWEKIEGLLGIGNIADPENWDLKHHVETAIKAHSLYKKD
                     VQYVVKDGEVIIVDEFTGRLMPGRRWSDGLHQAVEAKEGVNIRREDQTLATITFQNYF
                     RLYKKLAGMTGTAETEAAEFDKIYKLDIVVIPTNKPLLRIENSDVVFRTAKEKYYAVA
                     DEIAKLHENQQPVLVGTTSIEKSELLSQILARKGVRHVVLNAKFHEREAEIVAQAGRI
                     GMVTIATNMAGRGTDILLGGNPDFVARQELVKKAMARAISPAEGAINPMAAPGMLRFY
                     YQGQEFETSQENWDQVYQGHAAAAGAEHDQVIGAGGLFILGTERHESRRIDNQLRGRA
                     GRQGDPGASRFYLSLEDDLMRIFAREWVSTLLQKMGMEEGMPIESRMISNRIEAAQKA
                     VESQNFEARKHLLEYDDVMNKQREAVYGLRRQLLEGLDQKELIVEDYVPNILSNLLDE
                     FANERVHPDQWDVKGLKEKLSAHFGFDLDAESITPTEMTRHELGETIFDRLKGRYEAK
                     EQLIGDQQMRFHERMIMLSVIDGLWKDHLLNMDHLKEGIGLRGYAQQDPLVAYKKESF
                     EMFEAMMDRFQEDAVRFLFLMRIVGPDGQPVEIPSRPRAAVPAAPQVASADRLSGDGN
                     GHRMPNAPPVPLPPSRAPSTTIDDIEKEFQRKKQKELEHARMAGGG"
     gene            complement(3088..3774)
                     /locus_tag="VHE33_02825"
     CDS             complement(3088..3774)
                     /locus_tag="VHE33_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96411.1"
                     /translation="MSPHLSELFAEEYRAIRPRAPIPPMDVRFRRFTSLNTTIRLREG
                     RIRVSLSDLLEGAPEPVLRAIAHILLAKLYRKPIDGGHANRYRRFTTSEAMVRQSERI
                     RQTRGRKKILTAQGDHYDLDEVFETLNRQFFHGLLGRPLLTWSEVTARRLLGHYDSAH
                     NTIMVSRVFDRKGTPRYAIEYLMYHEMLHLKHPVKQKNGRRCVHSAAFQAEERLFPEL
                     ERAKAYLRTL"
     gene            3874..5172
                     /gene="aroA"
                     /locus_tag="VHE33_02830"
     CDS             3874..5172
                     /gene="aroA"
                     /locus_tag="VHE33_02830"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HVT96412.1"
                     /translation="MLTTVTERLIPPARNIQGSLRLPGDKSISHRYALLGGLAEGTTR
                     LTNFSTGADCASSLACVEALGAHVLRDGTDIAITGTAGRFRAPNQPLDCGNSGSTMRM
                     LSGLLAAQSGTFTLTGDASLSRRPMERIRKPLAAMGAQLDLTDGHAPITIHGAPLTAI
                     DYTTPIPSAQVKTALLFAGLQAQGATTVREAVRTRDHGELALRAFGAHVDRSRDSVSI
                     AGGQQLRAIDAAVPGDLSSAAFFLCAAALFPGSNLIFDELGLNPTRATLLDVLTALGA
                     HIGVLNLETKNDELVGTVQVNAPPAGLTGTSISGALSAQLIDELPALAAIGPYTQQGI
                     RIRDAKELRVKESDRIALVAANLRAMGAEVEEFDDGLDVPGGQTLHGATIDAGGDHRI
                     AMAFSVAALRATGDTLIRGAESAAISFPEFFDLLDRVAER"
     gene            5450..5608
                     /locus_tag="VHE33_02835"
     CDS             5450..5608
                     /locus_tag="VHE33_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96413.1"
                     /translation="MRRTVNGRLMEHRWPAYNAQGPNRSVRVNLSVDDHLAGNPRRSR
                     DGGIHRIF"
     gene            complement(5776..7701)
                     /locus_tag="VHE33_02840"
     CDS             complement(5776..7701)
                     /locus_tag="VHE33_02840"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96414.1"
                     /translation="MRVCCLPAVVLGLVSMGFAQSAAGPQPVPMPAPIAAPVDTPYPG
                     TIALDVNLTNTTDRVAHVHETIPVKAGELTLLYPQWIPGNHSPTGPIQAVAGLFVKAN
                     GKDIPWLRDRVDVYAFHIDVPKGVSSLDVDFDYLSPIRPQDGRVTMSHEMLDLSWNTV
                     VLYPAGHFSRDIQFNPTVKLPEGWKFATGLETASQDGGSVHFKETPLNKLVDSPLIAG
                     EYFKRVDLSTGPDNPVFLDVVADEPKDLEMTPEEVQAHKNLAIQAQKLFASHHYDHYD
                     FLFMLTNVLGGEGLEHHQSSEDGTRANYFTDWANGVRGRDLLGHEYTHSWNGKFRRPA
                     DLWTPNFNVPMRDDLLWVYEGLTQYWGLVLTARAGMETPEQARDELAGVAAGYEAARG
                     RDWRPLVDTTNQPTVSQRRPVSWVSWLRPEDYYEEGQLIWLDADTKIRELSGGKKSLD
                     DFAKLFYGVHNGSYITDTYTFEDIVNALNEVQPYDWATFLKTRVYQLHPEVPENGFTQ
                     GGYKLVYNDTIPAWEKHAPASPRYGTSFATSLGFSAMGDGTLGNVWWDSVAYKAGLVP
                     GMQLLAVDGQVYSADKLRESIKKAEKGTEPITVLVKDRDTVKSVALDYHGGLRIPHLE
                     RVEGTPARLDEILNPVQ"
     gene            7858..>8570
                     /locus_tag="VHE33_02845"
     CDS             7858..>8570
                     /locus_tag="VHE33_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HVT96415.1"
                     /translation="MASRRAFLKSGAVASIAATLPASAQEKTSAAGPSRIRQSVCRWC
                     YKQLSVDQLCQWGVQLGLKGIDLLQVEEFEIPRHYGLICTMGYAGGGTIPDALNRTEN
                     HASIEAAFRKNIPLAAKAGVPNVITFSGVRRGMSDDEGARNTIAGLNRVKSIAEDNGV
                     TICLELLNSKVNHPDYMADHTAWGARVMHEVNSPHVKLLYDIYHMQIMEGDLIDTIRQ
                     NIGVLGHFHTGGVPGRHEL"
BASE COUNT         1588 a         3017 c         2224 g         1741 t
ORIGIN      
        1 cctccgcctg ccatccgtgc gtgttccagt tccttctgtt tcttccgctg gaactccttc
       61 tcgatgtcgt cgatggtcgt cgaaggcgct cgagagggcg gcagcggaac aggaggcgcg
      121 ttcggcatgc gatgtccgtt accgtcgccg ctcagacgat ctgccgaagc gacctgcggg
      181 gccgccggaa cggcagcgcg tggacgtgac gggatctcca ccggctgccc atccgggcca
      241 acgatccgca tcaggaagag gaatctcaca gcatcttcct ggaagcgatc catcattgct
      301 tcgaacatct cgaaggattc cttcttgtaa gccaccagcg gatcctgctg tgcatacccg
      361 cggagcccaa ttccttcctt caggtggtcc atgttcaaca ggtgatcctt ccacagcccg
      421 tcgatcacgc tcaacatgat catccgctcg tggaagcgca tctgctgatc gccgatcagc
      481 tgctccttcg cttcgtatct gcccttgagc cggtcgaaga ttgtctcgcc caactcgtgt
      541 cgggtcatct cggtcggtgt gatgctctca gcatcgagat cgaatccaaa gtgagcgctc
      601 agcttctcct tcagtccctt cacatcccac tgatcggggt gaacgcgctc gttggcgaac
      661 tcatcgagca aattgctgag gatgttcgga acgtaatcct caacgatcag ttccttctga
      721 tcgaggcctt ccagcaactg gcgacgcaaa ccataaacgg cttcgcgctg cttgttcatc
      781 acgtcgtcgt attcaagcag atgcttgcgg gcctcgaagt tctggctctc tactgccttt
      841 tgtgccgctt cgatccggtt cgagatcatg cgcgactcga tgggcatgcc ttcttccatc
      901 cccatcttct gcagcagggt tgaaacccac tcgcgcgcga agattcgcat caggtcgtct
      961 tcgagggaca agtagaagcg cgatgcgccg gggtcaccct gacgtccggc gcgtccgcgc
     1021 aactggttgt cgatgcggcg cgactcgtgc cgctctgtgc caaggatgaa caagccaccc
     1081 gctccaatga cctgatcgtg ttcggctccc gcggcggctg catgtccctg gtaaacctgg
     1141 tcccaattct cctggctggt ctcaaactcc tgaccctggt agtagaagcg cagcatgccg
     1201 ggcgccgcca tgggattgat ggcaccttca gccggggaaa tcgcccgcgc catcgccttc
     1261 ttcaccaact cctgccgcgc cacaaagtca ggattgccgc ctaacagaat gtccgtgccg
     1321 cggcccgcca tgttcgtggc gatcgtcacc atcccgatcc ggcctgcttg tgcaacgatt
     1381 tcggcttcgc gctcgtgaaa tttcgcattc aagaccacat gccgcacgcc cttgcgcgcc
     1441 aggatctggg agagcaactc gctcttctcg atcgaagtgg tacctaccag caccggctgc
     1501 tgattttcgt gcagcttcgc aatctcatcg gcgacagcgt agtacttttc cttcgccgtc
     1561 cggaaaacga cgtcggaatt ctcgatgcgc agcagcggct tgttcgtggg gatcacgacg
     1621 atgtccagct tgtaaatctt gtcgaactcg gctgcctcgg tctcggccgt acccgtcatg
     1681 cctgccagct tcttgtacag ccggaaataa ttctggaacg tgatcgtcgc aagcgtctga
     1741 tcctcacgcc ggatgttcac gccctccttg gcttccaccg cctgatgcaa cccgtcggac
     1801 cagcgccgcc cgggcatcag gcgcccggta aactcgtcga cgataatcac ctcgccgtcc
     1861 ttcaccacat actggacgtc cttcttgtac agcgagtgtg ccttgatggc cgtttccacg
     1921 tggtgtttca ggtcccagtt ctccggatcg gcaatgttgc cgatgcccag cagcccttcg
     1981 atcttttccc atccctcatc ggtcaccgcg atcgtcttgt gcttctcatc gaccacgtag
     2041 tcgccggtca ggatcttctc ttccgggctc ttctcgatct cctcgcccat ttccagcgac
     2101 ggaatgatcc gattcacgcg cgcatatttg tcggtcgtct gatcggttgg ccccgagatg
     2161 atcagcggcg ttcgcgcttc gtcaatcagg atcgagtcca cctcatccac gattgagaaa
     2221 aagtgcccgc gctgcacaca atcgttcagc tcgaacttca tgttgtcgcg caggtagtcg
     2281 aagccgaact cgttgttggt accgtacgtg atatccgaac cgtaggcctt gcgccgctgg
     2341 ttgtcgtcca ggtcgtgaac gatcacgccc accgtcagac cgaggaactc gtaaatcttc
     2401 cccatccact ccgcgtcgcg cttggccaga tagtcgttga ccgtcaccac atgcacgccg
     2461 tgccccgcca gcgcgttcag atacacgggc agcgttgcta ccagagtttt tccttcaccg
     2521 gtcttcattt cggcgatctt gccctggtgc aggacgattc cgcccaccag ctgcacgtcg
     2581 aaatgccgca tgttcacggc gcgccatccc gcctcgcgca ccaccgcaaa cgcctccggc
     2641 agaagatcgt cgagcgcctg cttctctgcg gctactcgcg cctcttcgtc cgtaatccct
     2701 tccagggcgg acgcaatgcg cgcgcggaac tcggccgtct tcgcgcgcaa ctgctcgtcg
     2761 ctcatcgcct gcacttgcgg ctcgagcgca ttgatttctg ccacgcgcgg cagcaggcgc
     2821 ttcaccgccc gctcattgct ggtcccaaaa accttgttga gaactttagt tccgatcaaa
     2881 attgattcct ttggcgatct ttctcagcgc ccggcgagcc agtacttccc aatcgccgtc
     2941 ccagggagaa cttcaggatt ccttcctcat tttaaggtac ccacgcgatt cacgctgacg
     3001 ccagcaccga ccgcgtgtcc gctaaccgtt ttttcgctga ccgcttttca gctgaccgga
     3061 ctttagccga ccggaccttc gctggtctta gagcgtcctc agatacgcct tggccctctc
     3121 cagttccgga aacagccgct cttctgcctg aaaggccgcc gagtgcacgc accgccgccc
     3181 gttcttctgc ttcacaggat gcttcaggtg cagcatctcg tgatacatca ggtattcgat
     3241 cgcgtagcgc ggcgttccct tgcgatcgaa cacccggctc accatgatcg tgttgtgcgc
     3301 cgaatcgtag tgccccagca gccgccgcgc cgtcacctcg ctccacgtga gcagcggtcg
     3361 tcccagcagc ccgtggaaaa actgccggtt cagcgtttcg aacacttcgt ccagatcgta
     3421 gtggtcgccc tgagccgtaa ggatcttttt ccgtccccgc gtctgccgga tgcgctcgct
     3481 ctgccgcacc atcgcctcgc tcgtggtgaa ccgtcgataa cgattcgcgt ggccgccgtc
     3541 aatcggcttg cgatacagct tggccagcag gatgtgcgcg atcgccctta gcaccggctc
     3601 cggcgcgccc tccagcagat cggacaggct tacacgaatc ctcccttccc gcaaacggat
     3661 cgtcgtgttc agcgacgtga accgccgaaa tcgcacatcc atgggaggta tcggagctcg
     3721 cggccggatc gcccgatact cctcggcaaa cagttcggac aaatgggggc tcactacgat
     3781 cccgaatctc gcatagcgaa gcaaggaaat caagccggcg cgctgtgctg tgctccgggc
     3841 tctgtcccgt ggcccgtgca ttaccctgta atcatgctca cgacggtgac cgaacgcctc
     3901 atcccgccgg cccgcaacat ccagggcagc ctgcgcctgc ccggcgacaa aagtatcagc
     3961 caccgttacg cattgctggg tggcctggcc gaaggcacga cgcgcctgac caatttctct
     4021 accggcgccg actgcgcctc ctccctggcc tgcgtcgaag ccctcggcgc ccacgtcctt
     4081 cgcgacggga ctgacatcgc cattaccggc actgccggcc gcttccgcgc tccgaaccag
     4141 ccccttgact gcggcaactc cggctccacc atgcgcatgc tctccggttt gctcgctgcg
     4201 cagtccggaa ccttcaccct caccggcgac gcctccctct cgcgccgccc catggagcgc
     4261 atccgcaaac ccctcgccgc catgggcgcg caactcgacc tgaccgacgg ccacgccccg
     4321 atcacaatcc acggcgcgcc gctcacggcg atcgactaca ctacgccgat ccccagcgcc
     4381 caggtcaaaa ccgccctcct cttcgccgga ctccaggctc agggcgcgac caccgtccgc
     4441 gaagccgtcc gcacccgcga tcacggtgag ttagccctgc gcgccttcgg agcccacgtc
     4501 gaccgctcca gagactcggt ctccatcgcc ggcggtcaac agctacgagc aatcgacgca
     4561 gcggtccccg gcgacctctc ctcggccgcc ttctttcttt gcgccgccgc gctcttcccc
     4621 ggctccaatc tgatcttcga cgagctcggc ctcaacccca cgcgcgccac gctgctcgac
     4681 gtgctcaccg ccctcggcgc gcacatcggc gtcctcaacc tcgaaacgaa gaacgacgag
     4741 ctggtcggca ccgttcaggt caacgcgccg cccgccggtc tcaccggcac cagcatctcc
     4801 ggcgcgctct cagcccaact catcgatgag ctgcctgccc tcgccgccat cggtccctac
     4861 acacagcaag gcatccgcat ccgcgacgcc aaagagctgc gcgtgaagga gtcggaccgc
     4921 atcgccctgg tcgcggccaa tctgcgcgcc atgggcgccg aggtggaaga gttcgacgac
     4981 ggcctcgacg ttcccggcgg ccaaaccctg cacggcgcca ccatcgacgc gggcggcgat
     5041 catcgcattg ctatggcttt ctccgtcgcc gcccttcgcg ccacaggcga tacgctcatc
     5101 cgcggcgccg aatccgccgc catctcattc ccggagttct tcgatctgct cgaccgcgta
     5161 gcagaaagat aagttgtcag tcggttcgaa ccgaacattt ccagagccaa aggcacttca
     5221 ctgatcgcgt cttttttttc gttatcgtga ctgcaggatc gcagaggcaa atcttgggag
     5281 ctttgaccag cacccgcgtc atacggttac caggcgtctc ttcaagacgt tcgaaggagg
     5341 agggcttgga aaatccttcg caacaatagc cttcgtcgac gaattcgaaa gaataaacga
     5401 tccggtattc ctgcaccatc tcaggacagc ccgggcacct aaaggttgaa tgcgacgcac
     5461 tgtcaacggc cgcctgatgg agcatcgctg gcccgcttac aacgcgcagg gacccaaccg
     5521 atccgtccgc gtgaatctca gcgttgacga ccacctcgcc ggaaacccgc gccgctcgcg
     5581 cgatgggggg atacatagga tcttctaagt ggaccagagc cgggccaacc ggctgtttcg
     5641 tcggagtatg agtcgtctgc gccgcagcga cctgtcccac tgctaatgtg acgccatagc
     5701 taacgacccc gcaaaggaca acccggagaa gcataggcac catcaccctc ttctgccgac
     5761 taccctacac cctcattact ggaccggatt caaaatctcg tccagccgcg ccggcgtccc
     5821 ctctacacgc tccagatgcg gaatccgcag accgccgtga taatcgagcg caaccgactt
     5881 caccgtgtcc cgatccttca ccagcaccgt gatcggctcg gtccccttct ccgctttttt
     5941 gatcgactct cgcagcttgt ccgccgagta cacctgccca tccaccgcca acagttgcat
     6001 tcccggcacc agccccgcct tgtaggccac gctgtcccac cacacgtttc ccagcgttcc
     6061 atcgcccatc gcactgaatc caagcgacgt cgcaaagctc gtcccatacc gcgggctcgc
     6121 aggcgcgtgc ttctcccacg cgggaatcgt gtcgttgtac accagcttgt atccaccctg
     6181 cgtgaaaccg ttctccggca cctccggatg cagctgatac acccgtgtct tcaggaacgt
     6241 cgcccagtcg tacggctgca cctcgttcag cgcgttcaca atgtcctcga aggtataggt
     6301 atccgtgatg tagctcccgt tgtgcacgcc gtagaagagc ttcgcgaagt catccagcga
     6361 cttcttcccg ccgctcagct cgcgaatctt cgtgtccgca tccagccaga tcagctgccc
     6421 ctcctcgtag tagtcctctg gacgcagcca gctcacccaa ctcaccggcc gccgctgtga
     6481 aacggtgggc tgattggtcg tgtccaccag cggccgccag tcgcggcccc gcgccgcctc
     6541 atatccggcc gccacgccgg ccaactcatc gcgcgcctgc tccggcgtct ccattccggc
     6601 ccgggccgtc agcaccagtc cccagtactg cgtcagcccc tcgtagaccc acagcaggtc
     6661 atcgcgcatg ggcacgttga agttcggcgt ccacaaatct gcgggcctgc gaaacttccc
     6721 gttccacgag tgcgtgtact cgtgccccag caggtcgcgg ccgcgtacgc cgttcgccca
     6781 gtccgtaaaa tagttcgccc gcgttccatc ttcgctcgac tggtgatgct ccagcccctc
     6841 gccgcccagc acgttggtca gcatgaacag gaaatcgtaa tgatcgtagt ggtgcgatgc
     6901 gaacagcttt tgcgcctgaa tcgccagatt cttgtgcgcc tgcacttcct ccggcgtcat
     6961 ctccagatcc ttcggctcat cggccaccac gtccagaaac accgggttgt ccggcccggt
     7021 cgacagatcg acacgcttaa aatactcgcc tgcaatcagc ggcgaatcca caagcttgtt
     7081 cagcggtgtt tctttgaagt gaaccgaacc gccatcctgg gacgccgtct cgaggcccgt
     7141 cgcgaacttc catccctccg gcagcttcac cgttgggttg aactggatgt cccgcgaaaa
     7201 atgccccgcc ggatacagca ccacggtatt ccacgaaaga tcgagcatct cgtgcgacat
     7261 cgttacgcgc ccatcctgcg gacgaatcgg cgacagatag tcaaaatcca cgtcaagcga
     7321 cgaaacgccc ttcggcacgt cgatgtgaaa cgcgtagaca tccacgcgat cgcgcagcca
     7381 tggaatatct ttgccattcg ccttcacgaa caacccggcc accgcctgaa tcggcccagt
     7441 cggcgaatga ttacccggaa tccactgcgg atagagcagc gtcaactcgc ccgccttcac
     7501 cggaatcgtc tcgtgcacgt gtgccacgcg atccgtcgtg ttggtgaggt tcacatccag
     7561 cgcaatcgtt cccggataag gcgtatccac cggcgctgcg atcggcgccg gcatcggaac
     7621 cggctgcggc ccggctgcac tctgggcaaa ccccatggag acaaggccaa gaaccacagc
     7681 aggaaggcag caaacccgca tcgtcaccac gttctgaaca atcctccagc gtcgatcggc
     7741 gttgcgaaaa attctacagg acagaattcc gcagcaatcg cgctcgctgg cctccgctga
     7801 aaattctccg cgagtcgcac gccccggctc cttttaagct gtactctggt tcctgtcatg
     7861 gcttcccgtc gcgcatttct caagtccgga gctgtcgctt ccattgccgc cacccttccc
     7921 gcgtctgcgc aggagaaaac ttctgccgct ggcccgtcgc gcatacgcca gtccgtctgt
     7981 cgctggtgct acaagcagct ttccgtcgac cagctctgtc aatggggcgt ccagctcggc
     8041 ctcaaaggca tcgacctgct ccaggtcgaa gagttcgaaa tcccgcgcca ctatggcctg
     8101 atctgcacca tgggctacgc cggcggcggc accatcccgg atgccctgaa tcgcaccgag
     8161 aatcacgcat cgatcgaagc ggccttccga aaaaacattc ccctcgccgc caaagccggc
     8221 gtccccaacg tcatcacctt ttccggcgtt cgccgcggta tgtccgacga cgaaggcgcg
     8281 cgcaatacga ttgccggact caatcgagtc aaatccattg ccgaagacaa cggcgtcacg
     8341 atctgcctcg aactgctcaa cagcaaggtc aaccaccccg actacatggc cgaccacacc
     8401 gcgtggggcg cacgcgtgat gcacgaagtg aattcaccgc acgtgaaact gctctacgac
     8461 atctaccaca tgcagatcat ggaaggggat ttgatcgata ccatccgcca gaacatcggt
     8521 gtcctcggcc attttcatac tggaggcgtt ccgggccgtc atgaactcga
//
LOCUS       DASZYN010000113         2379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_966357, whole genome shotgun sequence.
ACCESSION   DASZYN010000113 DASZYN010000000
VERSION     DASZYN010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966357"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VHE33_02850"
     CDS             complement(<1..781)
                     /locus_tag="VHE33_02850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT96416.1"
                     /translation="MTRTTEKVRVLYSFPHRLGAARICHTAWQQVYGLLGAGASVVVC
                     AGSLSRELPPEAVVRPTLARGRFRIPYRVLGTERACAIHDWIVAQRLKRLVGKIDIVH
                     AWPLGSIRTLETAARLGIPTVLERPNAHTRFAYEVVQKECEKLGIVLPPDHEHAYKAG
                     VLAKEEAEYNAAFRLLCPSDFVAQTFVDQGFSREKLVRDQYGYDESVYHSGQQVRDDQ
                     RGLTMLFVGGAAPRKGLHYALEAWLRSAASRKGKFMIAGAFV"
     gene            complement(815..1768)
                     /locus_tag="VHE33_02855"
     CDS             complement(815..1768)
                     /locus_tag="VHE33_02855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT96417.1"
                     /translation="MTSESAQRTCPVCAKPDPVRWMVAPDRYHGRTKEYQLLRCTSCS
                     MVWLDDPPSKAEMWMHYGPDYDRTISEAAKKPEHWFERRKEVLRLKPEGGAILDLGCA
                     SGGFLSAMKGPSWKLFGIEMSEEAASEARNRCGAEVFVGDILDAPFPAQSFDVITCFN
                     VFEHVYEPREVLVRVAKWLKPGGVFYTLMPNIDSAGRRVFGSYWYPLELPRHLYHFSP
                     ATLRKVAKSAGLREVSLETRRELFIESSTRFIVDDVLKKFGISRPPLCKLNDDVSLPF
                     RVLRKIYRLTLMQLFTLAASLAGPGETMAAVLAKDSGPREA"
     gene            complement(1846..>2379)
                     /locus_tag="VHE33_02860"
     CDS             complement(1846..>2379)
                     /locus_tag="VHE33_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96418.1"
                     /translation="TDPLLEGAVVLLCAGSMCRSLSSLFRVLYRVSGGAVMDNAQLLL
                     GLGVALLLWPFGPRIGFYGMVGGVHIVGQFLGLILMSLSLTSRFKGFNPGVLAPTLLR
                     FCVAAAVILGASVAARYVGVPWDVNPRMLDTIRLATAAAISLLVTVPALLFTGSVSRS
                     EARTMLNTVSRKFASPA"
BASE COUNT          529 a          782 c          642 g          426 t
ORIGIN      
        1 gaacgaaggc gcccgcaatc atgaatttgc ctttgcggct cgccgccgac cgaagccatg
       61 cttccagcgc atagtgcagg ccttttctcg gcgcggctcc tcctacaaac agcatcgtca
      121 gcccgcgctg atcgtcgcgc acctgctggc ctgagtgata cacgctttcg tcgtatccat
      181 attggtcgcg caccagcttt tcccggctga aaccctgatc cacaaacgtc tgtgcaacaa
      241 agtccgaggg gcacagcaaa cgaaacgcag cgttgtactc cgcctcttct tttgccagaa
      301 cgccggcttt gtacgcgtgc tcatgatccg gtggcagaac gatgcccagc ttttcgcatt
      361 ccttctgcac cacctcatac gcaaagcgcg tatgcgcatt cggcctctcc agaaccgtcg
      421 gaatccccag gcgcgccgcc gtctccagcg ttcggatcga acccagaggc cacgcgtgaa
      481 caatgtcgat cttccccact aaccgcttca gccgctgcgc aacgatccag tcgtggatcg
      541 cgcatgctcg ctcggttccc agcacccggt acggaatgcg gaacctgccc cgagccagcg
      601 tcggccggac caccgcttcc ggcggcagtt cccgagagag cgatcctgca cacaccacca
      661 cactggcgcc ggcgcccagc agtccataca cctgttgcca ggcggtgtgg cagatccggg
      721 ccgctcccag tcgatgggga aagctgtaga gaactctgac tttttcagtg gtgcgcgtca
      781 tggtagatac caacggaata ctgacaactc aggcctatgc ttctcgcggg ccgctgtctt
      841 ttgctaaaac ggctgccatc gtctcacccg gacccgcaag cgatgcggcc agggtaaaca
      901 actgcatcag agtcaggcga tagatcttgc gcagcaccct gaagggaaga ctcacgtcgt
      961 cgttcagctt gcagagcgga ggacgcgaga tgccgaactt cttcagcaca tcgtcgacaa
     1021 tgaaacgtgt gctggattca atgaagagct cgcgccgcgt ttccagcgag acttcccgca
     1081 gtccggccga cttcgccacc tttctcagcg tcgccggaga aaaatgatac aggtgccgcg
     1141 gcaattccag cggataccag tacgagccga aaacacgccg tccggcggaa tcaatgttgg
     1201 gcatcagcgt gtaaaacacg ccgccgggct tcagccactt cgcgacgcgg accagcacct
     1261 cgcgcggctc gtaaacgtgt tcgaaaacgt tgaagcacgt aatcacatca aaactctgag
     1321 ccggaaaagg cgcatcgaga atatccccga cgaaaacctc agcgccgcag cggttacgcg
     1381 cttcgctggc cgcttcctcc gacatctcaa tgccgaacag cttccacgat ggccccttca
     1441 tcgcgctgag aaagccgccg ctcgcacaac caagatccag aatcgctcca ccctcgggct
     1501 tgagacgaag aacttccttc cgccgctcga accagtgctc cggctttttg gctgcctccg
     1561 agatcgtacg gtcgtagtcg ggcccgtaat gcatccacat ttccgctttg gacggcggat
     1621 cgtcgagcca caccatcgaa caggaggtgc agcgcagcag ctgatactcc ttcgtgcgtc
     1681 cgtggtaccg atccggcgca accatccagc ggacgggatc cggctttgca caaacagggc
     1741 aggtcctctg agccgattcg ctggtcatag gtgcgtcaat gataagttcg tcgttggcaa
     1801 tcatggatag aggtgtcctt gaggatgttt cccgagcgct ggagatcagg ccggagatgc
     1861 aaattttcgg gaaacggtat tcaacatcgt acgcgcttcc gaacgggata ctgaccccgt
     1921 aaacaacaga gccggcaccg tgaccagcaa actgatcgcg gccgccgtcg caagtcgtat
     1981 cgtatccagc atgcgcggat tcacgtccca cggcactccc acataccggg cagcgactga
     2041 ggcgcccagg atcaccgcgg ccgcgacaca gaagcgaagc agcgtcggcg ccagcacacc
     2101 aggattgaat cccttgaagc gggaggtgag actcaggctc atcagaatca gccccagaaa
     2161 ctgtccgaca atgtgtaccc cgccgaccat gccgtaaaac ccaatcctgg gtccgaaggg
     2221 ccagaggagc aacgctaccc ccagaccaag gagcaactgt gcgttgtcca tcaccgcgcc
     2281 gcccgaaaca cgatagagca cgcggaacaa cgaactcagc gatcggcaca tgctgccggc
     2341 gcacagcaga accaccgctc cttccagcag cggatcggt
//
LOCUS       DASZYN010000114         2128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_645144, whole genome shotgun sequence.
ACCESSION   DASZYN010000114 DASZYN010000000
VERSION     DASZYN010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645144"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VHE33_02865"
     CDS             complement(<1..500)
                     /locus_tag="VHE33_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96419.1"
                     /translation="MKLPIRNHISSPLTLFIEPYCDQYEIPPEGEAIVTLEDGEPYSL
                     DCCPENLVTLWGDHGGDALVEIVSKEQNRVIHALSFVHGFLFQYGREGKAAARDLHDS
                     IDRSEKTDGYVGARFQAYKAFREGFRTKAAELEPRDARLRKWSAGRALAGAYRAGGVA
                     AYFNHRT"
     gene            620..1642
                     /locus_tag="VHE33_02870"
     CDS             620..1642
                     /locus_tag="VHE33_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012778812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HVT96420.1"
                     /translation="MEDVSVIGIDLAKSVFQIHGVDARGEVVVRQRLTRAKLLPFFAR
                     LPRCLVGMETCASANYWARELVALGHEVRLMPPQYVKPYVKRSKNDASDAEAICEAVT
                     RPTMRFAGVKSPEQQSVLVLHRTRSILTRQRTQLGNAIRAHLAEFGIVAPVGRQGLDR
                     LMAIIGDESDDRLPQAARACLTMLVDQYRLVLAHLLETDRAIRADSRATELGRRLQEI
                     PGVGPLVASALVATVPDPQMFKSGRSLSAWLGLVPRQHSSGGKERLGGITKAGDIYLR
                     RMLFVGAMAVIRHAERHGTRRPWLMKLLSRRHPRVAAIALANKIGRIAWAMMTSGERY
                     REPVAA"
BASE COUNT          428 a          637 c          661 g          402 t
ORIGIN      
        1 gttcggtgat tgaaataggc tgccacacct ccggcacggt aagcgccagc aagcgctcga
       61 ccggctgacc atttccgaag tctcgcatcg cgcggttcta gttcagccgc tttcgtcctg
      121 aatccttcac gaaatgcctt ataggcttgg aagcgagcgc cgacgtaacc gtccgtcttt
      181 tccgatcgat cgatagaatc gtgcaagtcc cttgctgccg ctttgccctc tcgaccatat
      241 tgaaaaagaa acccatgaac gaagctaagc gcatgaatta cgcggttctg ctcctttgaa
      301 actatttcca ccaacgcgtc gcccccgtga tcaccccaca gcgtcaccaa gttctcagga
      361 caacaatcga gtgaataagg ctctccgtct tccagggtca cgatggcctc accttcaggc
      421 ggtatctcgt actgatcgca gtagggctca ataaagagcg ttagaggtga ggagatatgg
      481 ttcctgattg gcagcttcat gacccggccc tccgtggccg gttgaaattg catcagcatc
      541 gggatgtccg caattgatgt cgtggacggc gctccacccg gccggcattt ttgccggtca
      601 gtgtggagag gaggacacga tggaggatgt ttcagtaatc ggcatcgatc tggcgaagtc
      661 ggtgtttcag attcacgggg tcgatgcgcg aggcgaagtg gtggtgcggc agcgattgac
      721 ccgagcaaag ctgctgccgt tctttgccag actgccgcgc tgcctcgtcg gcatggagac
      781 ctgcgcaagt gccaactact gggcgcgaga actggtcgct ctgggccacg aggtgcggct
      841 gatgccgccg caatacgtga agccttacgt gaagcggtcg aagaacgacg cttcggatgc
      901 ggaggcgatc tgcgaggcgg tcacccgtcc gaccatgcgg ttcgccgggg tgaagtcgcc
      961 ggagcagcag agcgtgctgg tgctccatcg cacgcgatcg atcctcaccc gccagcgaac
     1021 ccaacttgga aatgcgatcc gggctcatct cgccgagttc gggatcgttg cgcctgtcgg
     1081 ccgccaaggc ttggaccggc tgatggccat catcggcgac gagagcgatg accggctacc
     1141 gcaggctgcg cgtgcctgcc tgactatgtt ggtcgaccaa tatcggctgg tgctcgcgca
     1201 cttgctcgag accgaccgtg cgatccgcgc cgatagccgt gcgaccgaac tcggccgccg
     1261 cttgcaggag atccccggtg tcgggccgct ggtagcaagc gcgctggtgg cgaccgtgcc
     1321 cgatccgcag atgttcaagt cggggcgcag cttgtcggcc tggctggggc tggtcccgcg
     1381 acaacactcg tcgggcggca aggagcgcct cggcggcatc accaaggcag gcgacattta
     1441 cttgcgaaga atgctgttcg tcggcgccat ggcggtcatc cgtcacgccg agcggcatgg
     1501 cacccgccgg ccttggctga tgaagctgct gagccgccgc catccacggg tggcagcgat
     1561 cgcgctggcc aacaagatcg gccggatcgc ctgggcgatg atgaccagcg gcgagcgtta
     1621 ccgcgaaccg gtggctgcat aacgaggaac gaacttcaag aggttggcaa gagcaggaga
     1681 ctggctgacg caagctgccc gatgacggga ccgatgatcc ccaggaaaac ccattaacga
     1741 cccggcgcta ccaagcgcgt gcaattggtt gggacctacc ggatcggcgg acaaggcatc
     1801 atggccagcg gcacatggca acgccgcatc aacaggtcga agacatggcc gcatcccaaa
     1861 accgcagctg caacgctata atccaaatct tgctaacgga gcgccgtcca gacagggtcg
     1921 cgtgctgacc gtctgttccg ggtggtgagc ggacgtcagc ggttggaggt ctgatctcgc
     1981 acaaagtcaa cggcgatccc ggtgacatag ccgactggct caccagtttt ccctctcttg
     2041 atcggaaaga cattggcagc cgactgctga caagcggtgc gtgaaaactc tagatccttg
     2101 cccggctccg gaaggcagcg ctgaactt
//
LOCUS       DASZYN010000115        14626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_455102, whole genome shotgun sequence.
ACCESSION   DASZYN010000115 DASZYN010000000
VERSION     DASZYN010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 14626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14626
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455102"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VHE33_02875"
     CDS             <1..315
                     /locus_tag="VHE33_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96421.1"
                     /translation="YGGFDWPGVAVLGFFVLPLVLVVYESMFDLRRPWGTAAAVYVLI
                     GLTEGTLGHLLFEVAIKTPLYLLALSWVTNQIVLAIPSTGDRVVTSRLMNRASTLVPD
                     EE"
     gene            complement(523..1668)
                     /locus_tag="VHE33_02880"
     CDS             complement(523..1668)
                     /locus_tag="VHE33_02880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT96422.1"
                     /translation="MAYLLSHWPTVFGVHIRKYQAAIILRVFFIGSNSYGSNARSCAD
                     SLRRLGCDVFDIDQELYSPPLTSFTSKVGLRLVRSRLVAEFNRAVLDIAARYPHEIFL
                     AFKGTYLLPETLRTIRQLGASLYNFYPDTSAFNSGKWLPLSLPEYDCVFYTKPFWYRD
                     VIKRIPLKNGFFLPHGYDPVVHRPFSLSNRDYADYACDVSLIATHMPHKEGVLTKLMQ
                     LRPRLHLRIWGNRWTTHCKDSALRRWVQGFPVHGERYSRAICATKINLAIMGGKVEGA
                     SSGDLTTNRTYAIPATGGFMLHERNDEVLSLYREGVEIECFDTPEELAEKIDYYLAHP
                     EKLGRIAHAGHLRCVPAYSYDNRMADLLRWHEEHSSRPTVPSLEDPG"
     gene            complement(1739..2983)
                     /locus_tag="VHE33_02885"
     CDS             complement(1739..2983)
                     /locus_tag="VHE33_02885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVT96423.1"
                     /translation="MASAKPTRKIRLAYFVSHPIQYQAPLLRRIAQEPDIDLTVFFSS
                     DLSVRGYMDSGFGVQVQWDVPLLEGYKYEFLPRIGGGDTLGFARPLNWGIFNRLRKGR
                     FDVVWVFGYSRLANLQAILAARLLRLPVILRAESNLHDHVRSKPVLIAKDRLFAFLRN
                     SVSCVVPIGKKNRAYWEHYFGENFPSFTMPYAVDNGFFQQRAREAAHEREDFRRQLKL
                     EPGRPVILYASKLQSRKRCIDLIEAYIRLAPARGADPAAYLLIVGDGVERANLEARVR
                     DSGLSSIHFLGFRNQTDLPRFYDLCDAFVLPSYNETWGLVVNEVMNAGRPVIVTDQVG
                     CQPDLVHDGVNGFVYPAFNVDALSQCLRSLLENADLRATMGENSLRIIRQYGFDQDVD
                     ALRRALALLIPDPSGEKKPCPL"
     gene            3283..3912
                     /locus_tag="VHE33_02890"
     CDS             3283..3912
                     /locus_tag="VHE33_02890"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT96424.1"
                     /translation="MLSIVRKHFPRNRKSRILDIGCGHGALLYVLRQEGYSDLHGADG
                     SEEQVELARRLGIPGVELSQADDFLLACPDASADVVALFDVLEHLDRQELFDLLSEVR
                     RVLAPAGLCIGHVPNAGGVFGAVIRYGDLTHEQAFTASSIRQMFGALGFSEVRCFEDK
                     PVIHGVTSLLRRVIWEAGSAPFRLLHGAEATNLHPILTQNLLFVAKRDG"
     gene            3988..5004
                     /locus_tag="VHE33_02895"
     CDS             3988..5004
                     /locus_tag="VHE33_02895"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HVT96425.1"
                     /translation="MNRTEKKSARVLVTGAAGFLGSHLCDALLAEGHRVTGVDNFSTG
                     NSENLEHLKNEPRFELIEHDICAPFDFGAVDFVFNFASPASPVEYMRLGIETLRVGSD
                     GTRNALEVAKKYGAGFLHASTSECYGDPTVHPQTENYWGNVNPVGPRSVYDEAKRFSE
                     ALVMAYHRYCGVDTRLVRIFNTYGPRLHGSDGRVISNLMVQALQGHDLTVYGEGTQTR
                     SFCYVSDEIEGILRLSRSNEHLPVNIGNPEEWTILECARIVLEVTGSSSRIRFVPLPE
                     DDPAQRRPDITKARTLLGWEPKIDLRTGLQLCLEYFRDSVKRGERASVAGCSKGCESR
                     TAQA"
     gene            5180..5476
                     /gene="rpsP"
                     /locus_tag="VHE33_02900"
     CDS             5180..5476
                     /gene="rpsP"
                     /locus_tag="VHE33_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HVT96426.1"
                     /translation="MIRLARVGARKQPYYRVVVIEKDRARNGRSVEVVGTYNPRTSPA
                     SVDLKLERIEYWKGKGAQPSDRVAKLLRSGPSSAGQSPAPKKGAEESADASTAA"
     gene            5656..5949
                     /locus_tag="VHE33_02905"
     CDS             5656..5949
                     /locus_tag="VHE33_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HVT96427.1"
                     /translation="MTQKTEKPDNMQELVTQIAKALVDLPEAVVVEAVEREESTVLRL
                     RVAPTDVGKVIGKQGRTARSMRTILGAVSMKHQHRYTLDILEEDHQEGTESEE"
     gene            6098..6712
                     /gene="rimM"
                     /locus_tag="VHE33_02910"
     CDS             6098..6712
                     /gene="rimM"
                     /locus_tag="VHE33_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HVT96428.1"
                     /translation="MADRADPKLVLVAKLVRPHGRRGELIAEILTDFPERFHHRTRLV
                     VIPPERTATRPREMELENFWFLRSRIVLKFQGVDSINEAEALRGFGVAIPFAERAALE
                     SGAVYVSDLAGCRVIDLNRGGAEVGVVVDVDRDSSSVELLVVHRTESKSGEEILIPFV
                     KEYLVRVDAEHRRLEMRLPPGLLEINAPMTEEEKREAAESARRS"
     gene            complement(6717..7232)
                     /locus_tag="VHE33_02915"
     CDS             complement(6717..7232)
                     /locus_tag="VHE33_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96429.1"
                     /translation="MFRVIALLAGLSSVVAPALLAQNQSLCVFQQKQGHSADVDAGFD
                     SSLLTKELTAKIPAAMALSVVPISGFSSKEIEAEAQRRGCNWVVTLWRDQLGPDSPNY
                     GGTLGNTQQTTGKGSQLWIKGTKLGGDTLLEYSLRKADAHKAIAHGEGEEESTYAKFA
                     ESIVKKIEKEK"
     gene            7436..8101
                     /locus_tag="VHE33_02920"
     CDS             7436..8101
                     /locus_tag="VHE33_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96430.1"
                     /translation="MKWGIVLLVVVLGGIATTASAKKKKEEGLPKLFCNAQYVKVQTQ
                     EGDPTPSLAAEYPSDYNAAVGVENRIQHWGRYTVIDQPSLQPVDLVFVVWKERKTGNR
                     LPGQPTEMPGGGPRRPGSGPGGPGQYPGGPGQYPGQPPQGPGEAPGIGGGPGDASGAP
                     GPGVGIMFPVDDQMAVYVTQGDGSLGAVLWRHSEKDGLKEPNMPLFRKFADAVDDACS
                     DSK"
     gene            complement(8202..8612)
                     /locus_tag="VHE33_02925"
     CDS             complement(8202..8612)
                     /locus_tag="VHE33_02925"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVT96431.1"
                     /translation="MPSTPEDRPRRRSGPHGKSTSRSLQPRQASHPASARAASTFAAL
                     GDRTRLSLLARLGRGGPCSISQLTHGSHLTRQAITKHLRVLERARIVRSIRSGRHSLF
                     EVIPEPVHELRDYLDRVSAEWDQAITRLQSLVEG"
     gene            complement(8596..9078)
                     /locus_tag="VHE33_02930"
     CDS             complement(8596..9078)
                     /locus_tag="VHE33_02930"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVT96432.1"
                     /translation="MTTATRTQPHVIDRVEKSVELKAPLSRVWRALTDYNEFGAWFRV
                     QLDGPFKAGEVATGKVLHPGYEHLTWRATIQAIDPEQYFAFTWHPYAVDPAVDYSKET
                     PTLVEFHLEKTAAGTLLKITESGFSDIPDGRRNEAFLRNSEGWAIQMKNIEEHIAQHP
                     "
     gene            complement(9287..10645)
                     /locus_tag="VHE33_02935"
     CDS             complement(9287..10645)
                     /locus_tag="VHE33_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96433.1"
                     /translation="MAASDEPAQDRLRPGTSGILPLPEIFFAVVILPLVAIAIVEAHR
                     SFLHSPGADWNSSEWMIDYAAGFMRRGQGGTVLLRLLGVTGWSFFAVVSTLTTTAYLG
                     LCAIATLVTWRSRGPAIWRLVFLFNPLLLLAAAEYGSFARKDIFFLWGTLLHVWVCDW
                     VLKRHKCIPSERTRLALLEWTVVLTTSVLLAALHEGLFLFIWLPMNAVIAAWGMRQLY
                     PGRTAKLSGLAMAFAPSLAVAFASIRRHGDAATAQAICQSWRVAFPVNCTPGPQFPKA
                     IDALAWSMRRDVLGCLHFVWTAGNFYLVIFAVLATLAVAVLLIAVLALEPSAKLEHLL
                     AIATGSLVCAVPLFVLGTDWGRWLFLAGTSSVIVMMSATLRPALYGVLPATAQRFFDR
                     TSRSTGARLGSLRNLVERHPGLSLTLLLMLPLPALPIASAMLLANPLLIVIDFLARIH
                     GS"
     gene            complement(10682..11437)
                     /gene="map"
                     /locus_tag="VHE33_02940"
     CDS             complement(10682..11437)
                     /gene="map"
                     /locus_tag="VHE33_02940"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HVT96434.1"
                     /translation="MSIHTPEELAAMRESGRIVRLMLEAMKAAARSGVTTEELDEIGG
                     QLMRQNGAVSGPTTVYQFPGVSCISVNDEIVHGIPSDRRLRNGDLLKLDVTISKDGFM
                     ADAAETVIVGPPTTESSIGNRLALCARRAFHEAMQVARAGHRVRDIGRVVEKEVSASG
                     FHVIRDLCGHGIGRTIHEKPSVPNWPDPSASDILTEGLVITVEPIIASRSGKSLLAKD
                     GWTVRTADRGLAAHYEHTIVITKNEPVLLTAAA"
     gene            complement(11506..12303)
                     /locus_tag="VHE33_02945"
     CDS             complement(11506..12303)
                     /locus_tag="VHE33_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HVT96435.1"
                     /translation="MRILFVGDVFGSAGRRIVREHIGHVITGRGVDLVIVNGENAAGG
                     FGLTPTIADELFDLGAHVLTTGNHVWDKKEIIDYLKSAAPDSHERARRILRPANYAPD
                     TPGYGVYQGTLPSGDGYAVMSLQGRVFMANNEDPFRTADKLLSEITAKVIVVDFHAEA
                     TSEKCALGWYLDGRVTAVIGTHTHIPTADNRVLTRGTAYQTDVGMSGPYDSIIGVEKE
                     YVLERFLTGMPNKFEAAKGDPRMAALLIECDAATGHASSAERLLLGE"
     gene            12429..12842
                     /locus_tag="VHE33_02950"
     CDS             12429..12842
                     /locus_tag="VHE33_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HVT96436.1"
                     /translation="MAANTRFATGVHAMVLLAAEPDGLQTSEDVAGKLNINPVVIRRV
                     FSLLHRAGLLQSQKGPHGGSKLARSAKAITLADVYAALDGGDLFHTASLSGQTAKVGG
                     ALKKVFDGAEAAMRDQLEKTTLNDLAKKAAKKAKK"
     gene            complement(12851..14311)
                     /locus_tag="VHE33_02955"
     CDS             complement(12851..14311)
                     /locus_tag="VHE33_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HVT96437.1"
                     /translation="MSSEGQIQDPEIRSADTPPGGARSSEAAVFEQPAQPSHLGEEFR
                     ALLSLAIPVVLSELGWMTMTIVDLIMVGRLGPDAIGAVGLGNAIYYAPSLFGIGLLLG
                     LDTLVSQSWGAGRFDDCHRSLAQAIYIAMAFTPALMLAMLAAERLFTGSGVDPTVAGL
                     TRSYVSILNWGTFPLLVYGGFRRYLQGVGRVRPVTFALVSANLVNLGFNWTFIYGHFG
                     FPALGVAGSAWSTVLARIYMAGVLVYAAWAHENGRGHPLFAHWPRPDWARIRALLHLG
                     LPAASQVVLEVAAFGAATVMASHLTPIALATHEIVLSCAAYTYMVPLGISAAAAVAVG
                     HAIGAGNPARARRAGLLAIGLGAGFMALMAVLFLVIPEPIIHIWTRDRSVVVLGAHIL
                     AIVAGFQIFDGIQTVSTGALRGLGETRFPMLMNLTAYWILGLPFGALLCFRFGWGLAG
                     LWTGLTVALILIALLLIVRWMRDSAKVRVSTPAHLR"
     gene            14509..14598
                     /locus_tag="VHE33_02960"
     tRNA            14509..14598
                     /locus_tag="VHE33_02960"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14543..14545,aa:Ser,seq:gga)
BASE COUNT         3116 a         4199 c         4593 g         2718 t
ORIGIN      
        1 tatggtgggt ttgactggcc cggtgtggcg gtgctcggct tttttgtatt gccgctagtg
       61 ctggttgttt acgagagcat gtttgacctg cggcgtccct gggggactgc tgcggcagtg
      121 tacgtgctta ttggattaac cgagggaact ttgggacatc tgctttttga ggtcgcgatc
      181 aagactccgc tatatttgct cgctctgtcc tgggtgacga atcagattgt gcttgcgatc
      241 ccgtccacgg gggatcgggt cgtgacgtcg cgactaatga atagagcgtc cacattggtc
      301 cctgatgaag agtgacctgt agtcagtcag caaggtatga tcggagcgag ctgaagaggc
      361 tcaggtgtct gtgccttcga gccccttgag cgtacgtgcg agttgtgtct cgctgtcgta
      421 gccggagcgg acagctcgtt cgcggctacg gcggggcaat ggcttttcgg aattccggac
      481 cggtcaggta gaaacggcac ttataggtgc acacctcgac gtttatccgg ggtcctccag
      541 cgaggggact gtgggccttg agctgtgctc ttcgtgccaa cggagaaggt ctgccattcg
      601 attgtcgtag gaatacgccg gaacgcagcg aagatgccct gcatgcgcga ttcgcccgag
      661 cttttccgga tgagcgagat agtaatcgat cttttcggca agctcctccg gggtgtcgaa
      721 gcattcgatt tcgacgcctt cccggtagag cgatagaact tcatcgttgc gctcgtgcag
      781 catgaagccc cctgttgccg gaatggcata agtgcgattg gtcgtgaggt cgcccgacga
      841 agctccttca actttgccgc ccatgatagc caaattgatc tttgttgcgc agatcgctcg
      901 agaataacgt tcgccatgca caggaaagcc ttgaacccag cgtcgcaggg ctgaatcttt
      961 gcaatgtgtt gtccagcggt taccccagat acgaagatgc agtcgcggac gcaattgcat
     1021 caacttcgtc agcacgccct ctttatgggg catatgggtt gcgatgagac tgacatcgca
     1081 ggcgtaatcg gcgtaatccc tgttgctcag cgagaagggg cggtgcacta ccgggtcata
     1141 accatgcggc aggaagaagc catttttgag tggaatgcgt ttgataacat cgcgatacca
     1201 gaatggtttt gtgtagaaca cgcaatcata ttctggaagt gaaagtggca accacttgcc
     1261 ggagttgaaa gctgatgtat caggatagaa attatagaga gacgcaccca attggcgaat
     1321 tgttctcagg gtctccggca gaagataggt tcctttgaat gcgagaaaaa tctcatgcgg
     1381 atatctcgcg gcgatatcga gaacagctcg attaaattcg gcaacaagcc gactccgcac
     1441 tagtcggagg ccaaccttgc tggtaaaact cgtcagcgga ggagaataca actcctgatc
     1501 gatatcgaaa acatcgcagc ccagccgccg aagcgaatca gcgcatgacc gtgcgttgct
     1561 gccatatgag ttactgccga tgaaaaagac tcgcaagatt atcgctgcct gatactttct
     1621 gatgtggacg ccgaacacgg ttggccagtg agacagaagg tacgccattt tgtaaggcgt
     1681 agggaataac gcggcaagtt gaaaccttcg tggaagaacc taggggcaaa accgatggtc
     1741 aaagaggaca aggtttcttc tccccagaag gatcgggaat gagcaaggcc agggctctcc
     1801 gaagtgcatc gacgtcctgg tcgaagccat attggcggat gatgcggagg ctgttttctc
     1861 ccatggtcgc ccttagatct gcgttttcca ggaggctgcg cagacactgg gacagggcat
     1921 cgacattgaa tgccggataa acgaagccat tcacgccgtc gtgcaccagg tcgggctgac
     1981 agccgacctg atcggtgacg atgaccgggc gcccggcatt catgacctcg ttgacgacca
     2041 acccccacgt ctcattgtaa gaggggagaa cgaacgcatc gcaaaggtcg tagaagcggg
     2101 gcagatccgt ctgattgcga aaaccgagaa agtggatgct ggagagtccc gagtcgcgga
     2161 cacgggcttc gaggttagcc cgctccactc catcgcccac gatgaggagg taagctgccg
     2221 ggtccgcacc tctggctggg gcaagacgga tatacgcctc gatgaggtcg atacagcgtt
     2281 tccgggattg gagtttggag gcgtaaagga tcacaggccg cccgggttct agtttgagct
     2341 ggcgccgaaa gtcctcgcgc tcgtgagccg cttctcgagc ccgttgctga aaaaagccgt
     2401 tgtcgacggc ataaggcatg gtgaaggacg ggaagttctc tccgaaatag tgctcccaat
     2461 aagcccggtt ttttttgccg atgggaacga cacaggacac cgaattgcgc aggaaagcga
     2521 agagtctgtc cttggcgatc aggacaggct tcgaccggac atggtcatgc agattggatt
     2581 cggctcgaag aatgacgggg aggcgaagaa ggcgggcggc gaggatcgcc tggagattgg
     2641 cgagacggct gtatccaaac acccagacaa cgtcgaagcg gcctttgcgg agacggttga
     2701 agatgcccca gttcagcggt cttgcaaacc cgagagtatc gccccccccg atacggggga
     2761 ggaattcgta tttgtaacct tcgagaagag ggacgtccca ttgaacctga acgccgaagc
     2821 cgctgtccat atagccgcgc acactaagat cgctcgagaa gaagaccgtc aggtcgatgt
     2881 caggctcctg cgcaatgcgg cgcagtagcg gagcctggta ctgaatggga tggctgacga
     2941 aataggcgag acgtattttg cgagttggtt tggcagatgc catccggaat tcggcgtcgg
     3001 acgaagaggt ggaggatcgc atcgtaggcc ttgccgaaat ggagcgagcg tgcggtaatc
     3061 gacatggtaa catcgccagc aagcgccaac aatcgggtcc ataggatgtg tcccgatgac
     3121 gattttcgag tgaactcagc gcgttcacca ggcattgcga attccggaca aaaccatttg
     3181 aacagctgga aagaagttct gtatcggtcg tacgtttcga gcggacaggt ccgttacacg
     3241 ggcggtacgg cggacgagtt tctaagaagc cgacgcccct atttgctgtc aattgttcgc
     3301 aaacattttc ccaggaaccg caaatcgaga attctcgaca tcggctgtgg tcacggtgcg
     3361 ttgctgtatg ttctccgcca ggagggttac agcgacctcc atggcgcaga tggatcagaa
     3421 gagcaggtgg aactggcgcg gcgacttgga attccgggag ttgaactgag ccaggccgac
     3481 gactttcttc tggcctgtcc cgacgcgagc gctgatgtgg tcgcgctctt cgacgttctt
     3541 gagcatctgg atcgtcaaga actgtttgac ctgctcagcg aggtaagacg cgtgctggcg
     3601 ccggcaggtt tgtgcatcgg gcatgtgccc aatgcaggcg gagtttttgg ggctgtcatt
     3661 cgctatgggg atctgacaca cgaacaagcc ttcacggcct catcgatccg tcagatgttc
     3721 ggagccctgg gattcagcga agtgagatgc ttcgaagaca aaccggtgat ccacggggtc
     3781 accagcctcc ttcgaagagt catttgggaa gctggcagtg caccgtttcg cctgttacac
     3841 ggcgcggagg cgaccaatct tcatcccatt ctcacgcaga atcttctttt tgttgcgaag
     3901 cgagacggat gaccattgcc caggaacgct gcgcctgacg atacaatgag tgacgtgcct
     3961 cgcccgcatg gagagcggcc ttccacgttg aatcgaacag agaagaagtc cgctcgcgtt
     4021 ctcgtcaccg gggcggcagg cttccttggg tcgcacctct gcgacgcgct cctggcggag
     4081 ggccatcggg tcaccggggt cgacaatttc agtaccggca actccgagaa cctcgaacat
     4141 ctgaaaaatg aaccccggtt tgagttgatc gaacacgata tctgcgcgcc gttcgacttc
     4201 ggcgccgttg atttcgtgtt caacttcgcg tcccccgcca gtccggtgga gtacatgcgg
     4261 ttgggaatcg agacgctgcg tgtgggctcg gatgggacgc gaaacgcgct tgaggtggcg
     4321 aagaagtatg gcgcggggtt tctgcacgcg tccacctcgg agtgctatgg agaccccacg
     4381 gttcacccgc aaacggaaaa ctactggggc aatgtgaatc cggtggggcc gcgctcggtg
     4441 tacgacgagg cgaagcgctt ctcagaagcg ctggtgatgg catatcaccg gtattgcgga
     4501 gtggataccc gtctggtgag gatcttcaac acctatggac cgcggctcca cggcagcgac
     4561 gggagagtga tatcgaacct gatggttcag gctttgcagg gacacgatct gaccgtctac
     4621 ggcgagggaa cacagacgcg gagcttttgt tatgtgtccg atgagatcga agggattttg
     4681 cgcctgtcgc gctccaatga acatctgccg gtgaacatcg gcaatccgga ggaatggaca
     4741 attctggaat gcgcacggat cgtgctggag gtgaccggat cgtccagcag gattcgtttt
     4801 gttcctttgc cggaagacga tcccgcgcag cgacggccgg atatcaccaa agcaaggacg
     4861 ctgctggggt gggagccaaa aattgattta aggaccgggc tgcaactttg cctggagtac
     4921 ttccgcgact cggtcaaacg cggcgagcgg gcatcggttg ccggatgttc caaaggctgc
     4981 gaatcgcgaa cggcgcaggc atagagacag gttttgcggt ggtccgtcgg gggtccaggc
     5041 ggtcgtcggg gtggtctgac cggtcgaacg agatgcagaa gtccctgcta tattaagaag
     5101 ctggattctt tgtttaccgg acacccgcca tgccggggat tccgggtttg caagccgaaa
     5161 ggacaactcg attgtgctga tgattcgtct ggcgcgcgtg ggcgcgcgca aacaaccgta
     5221 ttaccgtgtc gtcgtaattg aaaaagaccg tgcgcggaac ggacgttcgg tggaggtggt
     5281 gggtacctac aacccgcgca ccagcccggc atccgtcgat ctgaagctgg aacgtatcga
     5341 atactggaag ggcaagggag cgcagccgtc ggaccgagtg gcgaagctgc tgcgttcggg
     5401 tccttcgtcg gctggtcagt ctcccgcgcc gaaaaagggt gcggaagaga gcgcggacgc
     5461 ctcgacggcg gcctagaccg gcttcgttac ggaggagtcg actgctcgcc tgctccagat
     5521 acctcccaaa agtcactttt gtacttccag ggtcaggaag cctgcggatg gtagtttgga
     5581 ccactgcagt ccttacggta gtatccgcct aaccccggaa gttgaacgac gtgtgtcttc
     5641 ccgatggggg gcaggatgac tcagaaaaca gaaaaacccg acaacatgca ggaattggta
     5701 acccagattg cgaaggcgtt ggtcgatctt cccgaggcag tggttgttga agcggttgag
     5761 cgcgaggaga gcacggtgtt gcggctcagg gttgcgccta ccgatgttgg aaaggtgatt
     5821 gggaagcagg gacggacggc acggtcgatg cggaccatcc tcggcgcagt gagcatgaaa
     5881 catcagcacc gctacacgct cgatattctt gaagaggacc atcaggaagg caccgagagc
     5941 gaagagtagt ggacatgcga tgtcgacgcg ctcggcgcag gggtcacgcg cagaccgtgg
     6001 aagcagcgat tgtggaaacg gaacgccggg ttgcggatga acgcatcgac ataggggttg
     6061 agcgcgtcga tgctgttatc cagcgtgagg cagttgcatg gcagaccgcg cagatccgaa
     6121 attagttctg gtggcgaagc tggttcgccc gcatgggcgg cgcggcgagc tgattgccga
     6181 aatcctgacg gattttcctg agcgattcca ccacaggacg cggttggtcg tgattcctcc
     6241 cgagcgaacc gccacgcgtc cgcgcgaaat ggagctggaa aacttctggt tcctgcgcag
     6301 ccggatcgtg ctgaaattcc agggagtcga ctccatcaac gaggccgagg cgctgcgggg
     6361 attcggggtt gcgattcctt ttgcggagcg cgccgctctg gaaagtggag ccgtttacgt
     6421 cagcgacctg gccggctgcc gagtgatcga cctgaatcgc ggcggtgcgg aagtcggcgt
     6481 ggtggtggat gtggatcgtg attcttccag cgttgaactg ctggtggtgc accggacaga
     6541 aagcaaatcg ggcgaggaga tcctgattcc tttcgtgaag gagtatttgg tgcgggtgga
     6601 tgcggaacac cgccgactgg agatgcgatt gcccccgggg cttctggaga tcaacgcgcc
     6661 catgaccgag gaagagaaga gggaagcggc ggaaagcgcc cgacgctcgt gaacccctat
     6721 ttctccttct cgatcttctt gacgatcgat tccgcgaatt ttgcgtaagt cgactcctct
     6781 tcgccttcgc cgtgagcgat ggccttatgg gcatccgcct tgcgcagcga atattcgagc
     6841 agggtgtcgc ctcccagctt cgtgccctta atccagagtt ggctgccctt gcccgtcgtt
     6901 tgctgcgtat ttccaagcgt cccgccataa ttgggactgt cgggaccgag ctgatctctc
     6961 caaagtgtga caacccagtt gcagccgcgg cgctgcgctt ccgcctcaat ttctttcgaa
     7021 gagaagccgg aaatgggcac gacactcagc gccatcgcag cggggatttt cgcggtgagc
     7081 tctttggtca gaagactgga gtcaaatccc gcgtcaacgt cggccgagtg gccttgtttc
     7141 tgctggaaga cgcataagct ttggttctga gcaagcaggg cgggagcaac gaccgaagac
     7201 aatccagcga gcagcgcaat aactcggaac atcgtcagga accctccttt gagaaggtgg
     7261 caagtatacc gcagtgctgc tccgcttttc gggccgggat gacggaccgc cggccagggc
     7321 gtctatggag tgccggctgt gggagaaggg gggtcctgca cggcatgcgg tccgtcaacg
     7381 atacaatcgg gtgccgtaat gtgaagcggc ggaaacgctc cgccggaggc tgcgcatgaa
     7441 atggggcatc gtgctgctgg ttgttgtgct gggcggaatt gcaacgacgg cttcggccaa
     7501 aaagaagaag gaggaagggc tcccgaagct cttctgcaac gcccaatacg tcaaagtgca
     7561 gacgcaggaa ggagatccca ccccctcgct tgcggccgaa tacccatccg actacaacgc
     7621 agccgtcggc gttgagaaca gaatccagca ttggggtcgg tacacagtga tcgaccaacc
     7681 cagcctgcag ccggtagatc tggttttcgt cgtttggaag gaacggaaga cgggcaaccg
     7741 gcttcccggc cagccgactg aaatgccggg cggcggtcca cgccggccag gttcaggtcc
     7801 cggcggaccg ggacagtatc ccggcggacc tggacagtat cccggccagc ccccgcaagg
     7861 acccggagag gcacccggga taggtggggg accgggggac gccagcggag ccccggggcc
     7921 gggcgtgggc atcatgtttc ctgtggacga tcaaatggcg gtgtacgtga cccagggaga
     7981 cggaagcctg ggcgcagtcc tctggcggca ctcggaaaaa gatgggctga aggaaccgaa
     8041 tatgccgctc ttccgcaagt ttgccgacgc ggtggacgat gcctgctccg actcgaagta
     8101 gtcaggttgg ctttcgaccc gcatctccca taccgcgacc gacggaggag actcgcaaca
     8161 tcgtcgtcgc gacaaaggaa ctgccggcgc tcatcgccgc atcagccctc cacgagggat
     8221 tgcaggcggg tgatcgcctg gtcccactca gccgacacac ggtccagata atcgcgaagt
     8281 tcgtggaccg gctcagggat tacttcaaac agactatggc gcccggaacg aatgctgcgg
     8341 acaattctgg cgcgctcgag aacgcgcagg tgcttggtga tcgcctgacg cgtgagatgc
     8401 gagccatgcg tcaactggga gatcgagcag gggccgccgc ggcccagcct tgcgagcagc
     8461 gacaggcggg tccggtcgcc cagggcggcg aaagtgctcg ccgcacgggc agatgccgga
     8521 tgcgaggcct gcctgggttg aagactgcgg gaggtggatt tcccgtgggg tcctgagcgc
     8581 ctgcgtggcc ggtcttcagg ggtgctgggc aatatgctcc tcgatattct tcatctgaat
     8641 cgcccatcct tcactgttgc gcaggaacgc ttcgttgcga cgcccgtccg gaatatcgct
     8701 gaagccggat tcggtgattt tgagcagcgt gccggcagcg gtcttctcca gatggaactc
     8761 gaccagagtc ggggtttctt tggagtagtc gaccgcgggg tcgaccgcgt aaggatgcca
     8821 ggtgaaggcg aagtactgct ccggatcgat ggcctggatg gtcgcgcgcc aggtgagatg
     8881 ttcgtagccg ggatgcagga ctttgccggt ggcgacctcg ccggccttga agggaccgtc
     8941 aagctgaacg cgaaaccagg cgccgaactc gttgtagtcg gtcagggcgc gccagacgcg
     9001 agagagcgga gccttcagtt cgacggactt ctcgacgcga tcgataacgt ggggctgggt
     9061 acgggtggca gtggtcatag gcaacctccc ggttgcatat tactccgaag gctgtggaaa
     9121 tgcaaccaaa cggttgcatg agtttggaga ggtaaggatc gccgagtctg gctgatcgtt
     9181 tctggacatt gcccagttcc ggacatgtcg ctgcttcgcg gtccgacgcg agaaggctcg
     9241 gcaatcctgg ccgaaccaaa acggatggcc ggcaacgcag gcgtttttat gagccgtgta
     9301 ttcgagcgag aaaatcgata acgatcagca acggattggc gaggagcatg gcactggcga
     9361 tggggagagc cggaagcggc aacatgagca gcaacgtcag gctcagcccc ggatggcgtt
     9421 cgacaaggtt ccggagagag ccaagtctgg cgccggttga gcggctcgta cggtcaaaga
     9481 atcgctgtgc cgttgcagga agaacaccgt acagggccgg gcgcagagtc gcactcatca
     9541 tgacaatgac ggaggatgtt cctgcgagga agagccaccg gccccagtcc gttcccagga
     9601 caaaaagagg aacggcgcac accagcgagc cggtggcaat tgccaacagg tgttcgagtt
     9661 tggcggatgg ctcgagagcg agcacagcga tcagcagaac ggcgaccgcg agcgttgcca
     9721 ggacggcaaa aatcaccaga tagaaattgc cggcagtcca cacaaagtgc aggcagccga
     9781 ggacatcgcg gcgcatcgac cacgccagcg cgtcgatggc tttgggaaat tgcggacccg
     9841 gggtgcaatt gacggggaag gcgacacgcc agctttggca gatggcctgc gccgttgccg
     9901 catccccatg gcgccggatg gacgcgaagg cgacggccag actcggagcg aaagccattg
     9961 ccagtcctga gagcttcgca gtcctgcccg ggtagagttg ccgcatcccc caggcggcaa
    10021 taactgcatt catcggcagc cagataaaca ggaagaggcc ctcatgcagc gctgccaaaa
    10081 gaaccgacgt ggtgaggacc acagtccatt ccaaaagggc aagccgggtt cgctcgctcg
    10141 gtatgcactt gtgcctcttc aggacccagt cacagaccca gacatgcaga agcgtgcccc
    10201 agaggaaaaa gatatccttg cgcgcaaagg acccgtattc ggccgcggcg agaagcagga
    10261 gcggattgaa cagaaacacg agccgccaga tcgcaggtcc gcggctgcgc caggtcacca
    10321 gcgttgcaat cgcacagagg ccgaggtagg cggtggtcgt cagagtcgaa acgaccgcaa
    10381 agaagctcca tcccgtcacg ccgagcaaac gaagcagcac tgtcccgccc tggccccggc
    10441 gcatgaaccc cgcggcatag tcgatcatcc actccgaact gttccagtcg gcgccgggcg
    10501 aatgcagaaa gctgcggtgc gcctcaacga tggcgatggc caccagaggc aggatgacca
    10561 ctgcgaagaa gatctctggc aagggaagaa tgccggaggt tcctggccga agacgatcct
    10621 gcgctggctc atcggacgct gccatcgagg aacctggcat agaagtggtt ttgggcttct
    10681 ctcaggccgc ggcggtgagc aacaccggct cattcttcgt aatgacaatg gtgtgctcgt
    10741 agtgcgccgc gaggccgcga tccgcggtgc gcacggtcca cccgtctttg gcaaggagcg
    10801 acttccccga ccggctggcg atgatcggtt ccaccgtaat cacgagccct tcggtgagga
    10861 tgtccgacgc cgaaggatca ggccagttgg gaacgctggg tttctcatgg atggtccgcc
    10921 cgatgccatg gccgcaaagg tcgcgaataa cgtggaaccc ggaggcgctg acttcctttt
    10981 cgaccacacg tccgatgtcg cgcacccgat gcccggcgcg cgcgacctgc atggcctcat
    11041 ggaaagcacg gcgggcgcag agggcaagcc ggttgccgat cgaagactcg gtcgtgggcg
    11101 gcccgacgat gaccgtctcc gcagcgtcgg ccatgaagcc atccttcgag atggtgacgt
    11161 cgagcttcag cagatcgcca ttgcgcaacc gccggtccga cggaataccg tggacaatct
    11221 cgtcgttgac gctgatgcag gaaacgccag ggaactgata gacggtcgtc ggcccggaga
    11281 cggcgccatt ctggcgcata agctgcccgc caatttcatc cagctcctcc gtcgtcactc
    11341 cggagcgagc tgcggctttc atggcttcaa gcatgagccg gacgatgcgg ccggattcac
    11401 gcatggcggc gagttcttcg ggcgtgtgaa tagacaatgg aacttcctct gcttttattg
    11461 tctcaggcag cgcggcctgc gtgaaagcac atccggacca ggccgctatt cgccaagcag
    11521 cagccgttcg gccgagctgg catggccggt ggccgcgtcg cattcgatca gcagcgccgc
    11581 catgcgcgga tcgcctttgg cggcctcgaa tttgttgggc atcccggtca gaaaacgctc
    11641 cagtacgtac tccttttcga ccccgatgat cgaatcatac ggcccgctca tccccacatc
    11701 ggtctggtac gcagtgcccc tcgtaagtac gcgattgtcc gcggtgggaa tatgcgtgtg
    11761 cgtgccgatc accgcagtca cccgcccgtc gagataccat cccagcgcgc atttttccga
    11821 cgtagcctcg gcatggaaat cgaccacgat cactttggct gtaatctccg acagcagctt
    11881 gtcggcggtg cgaaacggat cctcattatt ggccatgaag actcgcccct ggagcgacat
    11941 caccgcatag ccatcgccgg agggcagcgt tccctggtaa acaccgtagc ccggcgtatc
    12001 gggcgcgtag ttggccggac gcagaatccg ccgggcgcgc tcatgactat cgggcgcagc
    12061 ggacttcaga taatcgatga tctccttctt gtcccataca tgatttccgg tggtcagcac
    12121 atgcgcgcca aggtcgaata actcgtccgc aatcgtcgga gtgagcccga atccgccggc
    12181 agcattttcc ccattcacaa taacgaggtc cacaccccga ccggtgatga catgaccgat
    12241 gtgttcccgc acgatacggc ggccggcgct cccgaacaca tcgccgacaa aaagaatgcg
    12301 catggcatca agattacagg agcagagcga gcctccacgg tcccgtcgaa ttgtccgcag
    12361 atggacagat gtacgacgtt gttacagatt tcatctgtcg ccgtagaagc tgtggtagcg
    12421 tgggatgcat ggccgcaaat acccgattcg caaccggcgt tcacgccatg gttttgcttg
    12481 ctgctgaacc ggatggactg cagacctcgg aagatgtcgc cggcaagctg aacatcaatc
    12541 ctgttgtcat tcgacgcgtg ttttctctcc tgcatcgtgc agggctcctg caaagccaga
    12601 agggtccgca tggcggatcg aagctggcgc gctcagccaa agccatcacc ctggccgatg
    12661 tctacgccgc tctcgacggc ggcgatctct tccacaccgc ctcgctctcc ggacaaactg
    12721 cgaaagtcgg cggtgcactg aagaaggtct tcgacggcgc cgaagccgcg atgcgcgacc
    12781 agctggagaa gaccacgctg aacgacctcg ccaaaaaagc cgcgaaaaag gcgaagaagt
    12841 agccttccga tcagcgcagg tgcgcgggcg tcgaaacccg caccttcgcc gaatccctca
    12901 tccaccgcac aatcagcaac agcgcaatca gaatcagcgc cacggtcagg ccggtccaca
    12961 aaccggccag accccacccg aagcggaaac acagcagcgc cccgaacggc agccccagga
    13021 tccagtacgc cgtcagattc atcagcatgg ggaagcgcgt ctcccccagc ccgcgcagcg
    13081 ctccggtcga caccgtctgg atgccgtcga aaatctggaa ccccgcgaca atcgccagga
    13141 tgtgcgcgcc cagcaccacc acgctgcggt cgcgcgtcca gatatggatg atcggctccg
    13201 gaatcaccag gaacagcacc gccatcagcg ccatgaatcc tgcccccagc ccaatcgcca
    13261 gcaggcccgc gcgccgcgca cgcgccggat ttcccgctcc gatggcgtga cccaccgcga
    13321 ccgccgccgc cgccgagatg cccagcggaa ccatataggt atacgcagcg cagctcagca
    13381 cgatctcgtg cgtcgccagc gcgatcggcg tcaggtgcga cgccatcacc gttgccgcgc
    13441 caaaggccgc gacctcgaga accacctgag atgcggccgg cagaccgagg tgcagcagcg
    13501 cgcgaatgcg cgcccagtcc ggtctgggcc agtgcgcgaa cagcggatgc ccgcgcccgt
    13561 tctcatgcgc ccacgccgcg tacaccagca cgcccgccat gtagatgcgc gccagcaccg
    13621 tcgaccacgc cgaccccgcc acgcccaggg cagggaagcc gaagtgcccg tagataaacg
    13681 tccagttgaa ccccagattc accaggttcg cgctcaccag cgcaaacgtc accggccgca
    13741 cccgccccac gccctgcagg tagcgccgga atccgccata gaccagcagc ggaaacgtcc
    13801 cccagttcag gatcgacaca tacgaccgcg tcagccccgc caccgtcgga tcgacgccgc
    13861 tgccggtgaa gaggcgctcc gccgccagca tcgccagcat cagcgccggc gtgaacgcca
    13921 tagcaatata gatagcctgc gccagcgacc ggtggcagtc gtcgaatcgc cccgcccccc
    13981 acgactgcga taccagcgta tcgagcccca gcagcagccc gatgccgaac agcgacggcg
    14041 catagtagat ggcgttgccc agaccgaccg ccccaatcgc atccggcccc agccgcccca
    14101 ccatgatcag gtcgacgatg gtcatcgtca tccagcccag ttcggacagg accaccggaa
    14161 tcgccagcga cagcaacgcg cggaactcct cgcccagatg cgaaggttgc gcgggctgct
    14221 caaagaccgc ggcctcggac gaccgcgcgc cgcccggcgg cgtgtcggcg ctccggattt
    14281 ccggatcctg gatttgacct tcggaggaca tggagagcgg cagggaactt gcctattgta
    14341 gaggggcgcg ggtgccgcat tcggatcgtc cctggcacaa gcacccgcac ctgcaccgcg
    14401 cccgcactgc accctgtgtc ctgcgccctg taccctgctc ttcgggtggt agattaatga
    14461 cagcgcgcaa ggcgcaaccg gtgctgctca gagcggcgcc gacgacgcgg agagatggcc
    14521 gagtggctga aggcgcacgc ttggaaagcg tgtatgctcg aaagggcatc cagggttcga
    14581 atccctgtct ctccgccata gcttgggcaa atcgccgatt ccaaac
//
LOCUS       DASZYN010000116         5658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_516635, whole genome shotgun sequence.
ACCESSION   DASZYN010000116 DASZYN010000000
VERSION     DASZYN010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516635"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="VHE33_02965"
     CDS             complement(<1..1347)
                     /locus_tag="VHE33_02965"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96438.1"
                     /translation="MRWRATAAGAVCGLLLVALAYGFPSQPRPSASPAEHKTAQMPAV
                     IFTAAPVYQSLAALYGRERFPRGAQLVILRSGQMLPLVPDFAATADANVSFDGKAVLF
                     AGKKTAQDPWQIWQIPVEGGTPQPVISAKTDLIRPLWMPDHRIVYARREAQGFALETA
                     GLDGSHTLRLSYLPGSFIPDDVLRDGRVLFEAQFPLGADATLQPVARSERSQAEMFLV
                     YADGSGVESVRCDHPDGLDHAGRDHGHQIGVGSSVADAGDIVFIQAGRLARFTSALAD
                     AAPVAAPSQDYAGEIAELPDGRWILSARRPGERHFAMSVWKPGAPALTRIAQDAERNL
                     VEPVVVAPRAVPNRHPSALHEWAYGNLLALDARQSRGGDLTTSPAMVRAETLDEDGRP
                     VSLGTAPVENDGSFFVQAAGDRPLRFILLDAAGHPLREEHGWYWVRRGEQRVCVGCH"
     gene            complement(1344..2207)
                     /gene="aroF"
                     /locus_tag="VHE33_02970"
     CDS             complement(1344..2207)
                     /gene="aroF"
                     /locus_tag="VHE33_02970"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF009239.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HVT96439.1"
                     /translation="MGGNWKDQFADAPDVLKRTPDDHTVVWAGGVAIGGDAFVTMAGP
                     CSVETERQLMETAERVAAAGARVLRGGAFKPRTSPYAFQGLGLEGLKLLARARQATGL
                     AVVTEVMAPAEAPMVAEYADILQIGSRSMENYSLLEAAAKTGRPILLKRGMVATIADL
                     LRSAQCIVSHGNPHVILCERGIRTFETATRNTFDIAGMVVLKEVCRLPVIADPSHAAG
                     RRSLVPALARAAVAAGADGLIVEVHPDPDHAWSDGEQSLTFNEFGTMMADLAPWCELR
                     ASCDCALETVA"
     gene            2397..3197
                     /locus_tag="VHE33_02975"
     CDS             2397..3197
                     /locus_tag="VHE33_02975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT96440.1"
                     /translation="MAANPSEQSIHEKVQQQFGATAAAYSTSTGHGDRAALAALVALA
                     APRATDNVLDIATGAGHTALAFAPHVQRVVAYDLTEAMLRETEKNAAERGLANVATRQ
                     GPAEKLAFVDAEFDIVTVRLASHHFADNATAVREMARVTQPGGKVVVVDNYGPEEEAL
                     DALFQHIEKTRDASHVRGYQLSQWREFLTGAGLTIEHEVTDHYSESPRGMDFDDWVRR
                     AKTPPDRVTELRRIFTSASPELRDWLQIRIEGDSIWFRIPQVTFIARK"
     gene            complement(3211..4458)
                     /locus_tag="VHE33_02980"
     CDS             complement(3211..4458)
                     /locus_tag="VHE33_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT96441.1"
                     /translation="MALVGTRSISQRRPRTGQPRRRQLVVRSVMLSGFPWKWVLIGVL
                     CAGHCTIQAQTAVSRQSPEVKQAMQLDQQGRYANAAQAWRGIAERNPRDAIAWASLGL
                     DLARQGKYPDAVSAYQKAMTLNPRIPGLQLNLGLALFKQGKLDEAIPPLKRALATAPV
                     GEQASVLLGMSYYGTARYAQAVPYLRAAVAKSPQNVEMRGVLAQSCLYAKQYNCVLDE
                     YKKILLANPDSAQAHMLAGEALDGLNQTDKAIAEFQAAARAAPTEPNVHFGLGYLLWK
                     QHRYPEAAEEFRKEIQNEPMHAQALAYLGDTEMKLEHTAEAEAILRRAVHQVGATPLA
                     WLDLGIVLAAQGKNEDAASDFRQAIQLNPETVDAHWRLARLYQATGKTEQARAEFAKA
                     RALHEKEDKALVQQMTPPVPSPH"
     gene            complement(4458..>5658)
                     /locus_tag="VHE33_02985"
     CDS             complement(4458..>5658)
                     /locus_tag="VHE33_02985"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HVT96442.1"
                     /translation="WSIDSAIDSCSAGEVHPLRLILGIACLVSALLDAFETVILPRRA
                     TGRFRLTKVFYVVTWRPWRWMSRQIRHPRKRETMLSFYGPLSLVVLIAMWAAALLLGF
                     ALIYSSEPNPFIDPRASTHSFVTDLYASGTTLFTLGLGDVVPHTLLVRCLAVLEAGMG
                     LGFVALVIGYFPVLYGAFSRREVNIALLDARAGSPPTAFELIKRHSFEGGGAALVLLL
                     EEWERWSAELLESHISYPLLCLFRSQHTSQSWLSALTAILDTCALLIAGVQEQSARQA
                     QLTFAMARHALVDLSQILSAEPMLNPADRLPAEKYAQIYDKLCEMGVRVCRDQQSAAR
                     LRELRELYEGYAEALSRYLEMPLPPFFIEHPKKDNWLTVARVRAEAEAGIQAASPALL
                     HDEDHLF"
BASE COUNT         1092 a         1878 c         1732 g          956 t
ORIGIN      
        1 gtggcagccg acgcagacac gctgttcgcc gcgccgcacc cagtaccaac cgtgttcctc
       61 gcgcagcgga tggcccgccg catcgagcag aatgaagcgc aggggccgat cgccggccgc
      121 ctggacgaag aacgatccgt cattctctac gggggctgtg ccgagcgaga ccggacgccc
      181 atcttcgtcc agcgtttcgg ctcgcaccat ggccgggctc gtcgtcaagt ctccgcctcg
      241 cgattgccgc gcatcgaggg ccagcagatt gccgtaggcc cactcatgca gcgccgaagg
      301 atgccggttc ggcaccgcgc gcggcgcgac caccacgggc tcgaccagat tgcgctcggc
      361 gtcctgtgcg atccgtgtca gtgccggcgc gccgggcttc cacacactca tcgcgaagtg
      421 ccgttcgccg ggcctccgcg cagacaggat ccaccggccg tccggcagct ctgcgatctc
      481 tcccgcgtag tcctgtgacg gagccgcgac cggggccgca tcggccagag ctgaagtaaa
      541 gcgcgccagc cgccctgcct ggatgaaaac gatgtccccg gcatcggcaa cgctcgaccc
      601 gacgccgatt tgatgtccgt ggtcccggcc tgcatggtcg aggccgtcgg ggtggtcgca
      661 gcgcacgctc tcgacgccgc tgccatctgc atacaccagg aacatttccg cctgggatct
      721 ctcgctccgc gccaccggct ggagcgtcgc gtccgccccc agaggaaatt gcgcctcaaa
      781 cagcacgcgt ccatcgcgca gcacgtcgtc gggaataaag cttccgggta gataggacag
      841 ccgcagtgtg tgggacccat cgagtcctgc ggtttccagc gcgaagccct gggcttcacg
      901 acgtgcgtag acgatgcgat gatccggcat ccataaaggg cggatcagat cggtttttgc
      961 gctgatcacc ggctgcggag taccgccctc gacggggatc tgccagatct gccagggatc
     1021 ctgcgctgtc ttcttccctg caaaaagcac ggccttcccg tcgaaggaca cgttggcatc
     1081 ggcagtggcg gcaaagtcag gaaccagcgg cagcatctgt ccggagcgca gaatgacgag
     1141 ttgtgcgcct cgcggaaaac gttcgcgtcc gtaaagcgca gccagtgact gatagacggg
     1201 cgctgctgtg aagatcacag caggcatttg agccgtctta tgttcggcgg ggctcgccga
     1261 cggtcgcggt tgggagggga atccatacgc gagcgcgacc agcaacaacc cacacaccgc
     1321 gcccgccgct gtcgcgcgcc atctcacgca accgtctcca gcgcgcagtc acaagaggcc
     1381 cgcagttcgc accagggagc gagatcggcc atcatggtgc cgaattcgtt gaaggtcagg
     1441 gactgctctc cgtcgctcca cgcatgatcc ggatcgggat gcacctcgac gatgaggccg
     1501 tcggcgcccg cggcaacggc ggcgcgcgcc agcgctggaa ccagggatct gcggcccgcc
     1561 gcgtggctgg gatcggcaat caccggaagc cggcagactt ccttcagcac gaccattccg
     1621 gcgatgtcga aggtattgcg cgtcgcagtt tcgaaggtgc gaatgccgcg ctcgcacaga
     1681 atcacatgag gattgccatg gctgacgatg cactgcgccg agcgcagcag atcggcgatg
     1741 gtggcaacca tgccgcgctt cagcagaatg ggccggccgg ttttggccgc ggcttcgagc
     1801 agagagtagt tttccatgct ccgcgagccg atctgcagaa tgtcggcgta ctcggcgacc
     1861 atgggcgctt ccgccggggc catcacctca gtgacaacgg caaggccggt cgcctggcgc
     1921 gctcgggcca gcaacttcag cccttcaaga cccaggccct ggaacgcata gggcgaggtc
     1981 cgcggcttaa aggctccgcc acgcagaacc cgcgcccccg ctgcggccac gcgctctgcg
     2041 gtttccatca gctgacgttc agtctcgacc gaacacggac ccgccatggt gacgaatgca
     2101 tcgccaccga tggcaacacc gcccgcccat acgacggtat ggtcgtccgg cgtgcgcttc
     2161 agaacatcgg gtgcgtccgc aaactgatct ttccagttgc cgcccacctc cactccactt
     2221 ctctggggaa cagctgagac tatcgctcgc actgggacga tgcctccacc caaagattga
     2281 attcgaccct gggtacgaac gcctgtgatg gcggtcacaa aacgcagtga gcaacgccct
     2341 caggcggggg agagttgcgg ttcgacagtc gccagcgagc cctctagcat gggtctatgg
     2401 cagcgaatcc gagcgaacag tccatccacg aaaaagtcca gcagcagttt ggcgcgaccg
     2461 ccgctgccta ctcaaccagc acggggcacg gcgaccgggc ggcactggcg gcgctggttg
     2521 cactcgcggc tccacgggcg accgacaacg ttctggacat cgcaaccgga gccggccata
     2581 ccgctctggc cttcgcgccg cacgtgcaga gagtggtggc ctacgacctg accgaagcca
     2641 tgcttcgtga aacagagaag aatgcagcag agcgcgggct ggccaacgtt gcgacgcggc
     2701 agggcccggc ggagaagctg gctttcgtcg atgccgagtt cgacatcgtc accgtgcgcc
     2761 tggcctccca ccacttcgcc gacaacgcca ccgcggttcg cgaaatggcg cgggtcacgc
     2821 aaccaggcgg caaagtcgtc gtggtcgaca actacggtcc ggaagaggag gcactcgacg
     2881 cgctcttcca gcacatcgag aagacgcggg atgcgtcgca tgttcgcggg taccagctca
     2941 gccagtggag ggagtttctg accggggccg gcctgacaat cgaacacgaa gtgaccgatc
     3001 actacagcga gagcccgcgt ggaatggact tcgacgactg ggttcggcgc gcaaagaccc
     3061 caccggaccg cgttacggag ttgcgccgga tctttacgag cgcctcaccg gaattgcgcg
     3121 actggctgca gattcgaatc gaaggcgact cgatctggtt tcgcatcccg caggtcacgt
     3181 tcatcgcccg aaagtaagca gcgtccgcgt tcagtgtgga gacggcaccg gcggagtcat
     3241 ctgctgcacc agcgctttgt cttccttttc gtgcagcgcg cgtgcctttg caaattccgc
     3301 ccgcgcctgt tcggtttttc cggttgcctg atacagccgt gccaaacgcc agtgcgcatc
     3361 tacggtttcc gggttcagct ggatcgcctg gcgaaagtcc gacgccgcat cctcgttttt
     3421 gccctgagcg gcgagcacga tgcccagatc gagccacgcc agaggagtcg ctcccacctg
     3481 atgcaccgca cgccttaaaa tcgcctcggc ctcggcggtg tgctccagct tcatctcggt
     3541 atcgcctaag tacgcgagcg cctgcgcgtg catcggctcg ttctgaatct ctttcctgaa
     3601 ttcctccgcc gcctccggat accgatgctg cttccagagc agatacccca gaccgaaatg
     3661 tacgttgggc tcggtcggag cagccctggc cgccgcctga aattccgcaa tggctttgtc
     3721 ggtctgattg agcccatcca gcgcctcgcc ggccagcatg tgcgcctgcg ccgagtccgg
     3781 attcgccagg agaatttttt tgtactcgtc cagaacgcag ttgtactgct tcgcgtagag
     3841 gcagctctgg gccaggacgc cgcgcatctc cacattctgt ggcgactttg ccaccgctgc
     3901 gcgcagatac ggaaccgcct gcgcgtaacg ggcggttccg tagtagctca tgcccagcag
     3961 gacgcttgcc tgttcaccca ccggggcagt cgcgagcgcg cgtttcagcg gcgggatggc
     4021 ttcgtcgagt ttgccctgtt tgaacagcgc cagtccgagg ttcaactgca ggccgggaat
     4081 gcgcgggttg agcgtcatag ccttctgata cgccgatacc gcatccggat attttccctg
     4141 ccgcgccaga tcgaggccga ggctcgccca ggctatggca tcgcgtggat tgcgctccgc
     4201 gatcccgcgc caggcctgtg ccgcgttcgc gtatcggccc tgctgatcga gttgcatcgc
     4261 ctgcttcacc tccggcgatt gcctcgagac ggctgtttgc gcctggatcg tgcaatggcc
     4321 cgcgcaaagc actccgatca ggacccactt ccatgggaaa cccgacaaca tcaccgaacg
     4381 gacaacgagc tgccggcggc ggggttgacc ggtgcgaggt cttcgctgcg aaatcgatct
     4441 cgtgcccacg agcgccatca gaacaaatgg tcctcatcat gcagcagggc tggagaggca
     4501 gcctgaattc ctgcctccgc ttcagcgcgc acgcgggcaa cggtcagcca gttgtccttc
     4561 ttcggatgct cgatgaagaa cggcggcagc ggcatctcca gataacggct cagagcctcg
     4621 gcatagccct catacagctc ccgcagttcc ctgagccgcg ccgcgctctg ctgatcgcga
     4681 cacacgcgca cacccatctc gcacagcttg tcgtagatct gagcgtactt ctccgccggc
     4741 agccggtctg ccggattcaa catcggctcg gccgagagga tctgtgacag atccaccagc
     4801 gcatgacgcg ccatggcaaa ggtgagttgc gcctgccggg cgctctgctc ctgcacgccc
     4861 gcaatcagca gcgcgcacgt atccagaatg gcggtcagtg cgctcagcca gctttggctg
     4921 gtgtgctgcg agcgaaaaag acaaagcagg ggatacgaaa tgtggctctc cagcaactcc
     4981 gcggaccagc gctcccactc ctccagcagc aaaacaagag ccgcgccgcc gccctcgaag
     5041 gaatgccgct tgatcagttc gaaggcggta ggcggcgacc ccgcgcgggc atcgagcagg
     5101 gcgatattca cttcgcgccg tgaaaacgca ccgtacagaa ccggaaaata gccgatcacc
     5161 agagccacaa aacccaggcc catcccggcc tcaagcacag ccaggcaacg aaccagcagc
     5221 gtgtggggca ccacgtcgcc caatccgagg gtgaacagcg ttgtgccgct cgcgtacaga
     5281 tccgttacga acgaatgggt cgaagcgcgc ggatcgataa acggattggg ctctgaagaa
     5341 taaatcaaag cgaagcccag cagcagtgct gccgcccaca tggcaatcag aaccaccagc
     5401 gacagcggcc cataaaagct gagcatcgtc tcgcgcttgc gagggtggcg tatctgtcgc
     5461 gacatccacc gccagggccg ccacgtgacg acatagaaaa ccttcgtcag ccggaatcgc
     5521 ccggtggcgc gccgcggcag aatcacggtc tcaaaggcgt cgagcagagc agacaccaga
     5581 cacgcgatgc cgagaatcag ccgaagaggg tgcacttcgc cagcagagca ggaatcgata
     5641 gccgagtcaa tggaccac
//
LOCUS       DASZYN010000117         7253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_904387, whole genome shotgun sequence.
ACCESSION   DASZYN010000117 DASZYN010000000
VERSION     DASZYN010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904387"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /gene="argC"
                     /locus_tag="VHE33_02990"
     CDS             <1..1094
                     /gene="argC"
                     /locus_tag="VHE33_02990"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HVT96443.1"
                     /translation="TAGTSTLNSAAVSPVAVMGVSGYAGMELARLLLRHPALAAKPPL
                     FLGRAESSPEPLAAIHPQLMDNNGTGLGAGALTIEPFSWELLQSRGVKVLFLATPHEQ
                     SREWVPQALEHGLRVIDLSGAWRLTHASNRAVYKFADEDSPIATHVQAKAVYGMPELH
                     RDEIRDAGVVANPGCYATSIILAVKPLIAAGWVDLDRGIICDSKSGVSGAGKAPTAKT
                     HFMHAADNLSAYGLFSHRHTGELLEQLDVSAGQIVFTPHLLPIPRGILSTIYIGFTEP
                     HQPDEVEALFRAFFAQSPLVRVFPAGQLPQIQYSVHTNYCDIGFHLAADGRRCIIVSC
                     LDNLLKGAAGQAVQNFNLMSGLKETDGLL"
     gene            1142..1924
                     /gene="argB"
                     /locus_tag="VHE33_02995"
     CDS             1142..1924
                     /gene="argB"
                     /locus_tag="VHE33_02995"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HVT96444.1"
                     /translation="MRYVVKLGGVTLENPELLHGCAKAIAELVKDGNQVAIVHGGGVQ
                     LTRTLKQLGKQSEFVAGLRITDAETRDTALMVLGGGVNKALVAALGSHGQPAVGLSGG
                     DGLVFRARKKRTAPDLGFVGEIAAADPRWLEAIWQMSAVPVISSIALGFDGEYYNINA
                     DEMAAACAGACKADALVFLTDVPGVLAADGSVMRWLSIDQIAELTRDAVITGGMLPKL
                     SACREALLNGVKRVRILPANVADLLPDLCSSRVNQGTEVMVA"
     gene            1969..3225
                     /locus_tag="VHE33_03000"
     CDS             1969..3225
                     /locus_tag="VHE33_03000"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002874.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HVT96445.1"
                     /translation="MKLEELQAAESRLLLGTYDRYPLLFERGEGAHLIDANGARYLDL
                     LSGIGVNALGYAHPAIEKAIADQSRKLIHISNLYYHEGQAALALRLTEATGMDRAFFC
                     NSGTEANEAALKLARASAKLQRENGRSLGTKFLALENSFHGRTMGSVAATWKDKYREP
                     FNPVMPGVEFVRFNDIDDLKAKFTTDVCAILVEVIQGEGGIRPVSKEFLQAARDLTHS
                     TGALLIADEIQSGMGRTGKFCAYQHYGIQPDVTTLAKPLAGGVPLGALLCTEEASQAI
                     HAGMHGTTFGGGPLACAVALAVLSTIERDNLLQHVTETGTYFEDKLAGLAQKHSAITE
                     VRGLGLMLAVELDSADLAKTVLGEMMQRRILINRTSETVLRFLPPYILEKAHVDESIT
                     ALDEILTEQTAQTAATSPSGGKRIGN"
     gene            3215..4207
                     /gene="argF"
                     /locus_tag="VHE33_03005"
     CDS             3215..4207
                     /gene="argF"
                     /locus_tag="VHE33_03005"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HVT96446.1"
                     /translation="MATKTIAVSELDSVSPDVSLAGRDLCSVADLTPAELAGILALAH
                     DVKVNPGAYRWALDAKQMVMFFEKQSLRTRLTFQTAMNTLGGSALFVDQTSSPLGERE
                     SLADIARNVERWVQILVLRTYAHETITEMAQYASVPVVNALSDLEHPCQAIADFMTLQ
                     ERFGDLKGFNFTYVGDGNNVCHSLMLTAALLGAHCTIGTPKNYAPKADIVAQARAIAN
                     VTGSNVALVNDPVAAVHGADAIYTDVCTSMGFEHEATKRAPIFKPYQVNEALMAQASP
                     SAVFMHCLPAHRNAEVTDAVLDGPQSVVFDQAENRMHAQKALLLMLLGAGKMQR"
     gene            4297..5649
                     /gene="argG"
                     /locus_tag="VHE33_03010"
     CDS             4297..5649
                     /gene="argG"
                     /locus_tag="VHE33_03010"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF003779.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HVT96447.1"
                     /translation="MSVILENLPVSQKVGIAFSGGLDTSAALHWMKQKGALPWAYTAN
                     LGQPDETDYDAIPRKALEYGAENARLIDCRGPLVREGIAALQSGAFHISTAGVTYFNT
                     TPIGRAVTGTMLVTAMKEDGVNIWGDGSTFKGNDIERFYRYGLLVNPSLQVYKPWLDP
                     AFIDELGGRAEMSAFLQKSGFDYKMSAEKAYSTDSNLLGATHEAKDLEQLSSSIRIVD
                     PIMGVASWRDDVAVKPEEITVRFHQGFPIALNSKEFSDPVELLLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGLALLFIAYERLITGIHNEDTIETYRENGRKLGRLLYQG
                     RWFDPQAIMLRESAQRWVSSAVTGQVTLELRRGNDYSILNTESPNLTFHPERLSMEKT
                     DSAFSPRDRIGQLTMRNLDITDTRAKLLTYAQSGLINLSNGAEMPQLNGGATKNEEAR
                     "
     gene            5646..7127
                     /gene="argH"
                     /locus_tag="VHE33_03015"
     CDS             5646..7127
                     /gene="argH"
                     /locus_tag="VHE33_03015"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HVT96448.1"
                     /translation="MTEPSSSAPTTKMWAGRFRATPDPQFEAWQRSLPFDGRLLPQEI
                     AASKAHAHTIAVAGILSPAELETTLRGLEAILARLAPQEPGSPFRATITSSQAEDIHH
                     FVELALTEEIGDLALKLHTGRSRNEQIATDLRLFVRDAIDATLAHLASWASALLDLAV
                     ESENAVMPSYTHLQRAEPVLVAHWLLAYVEMLLRDISRFEDCRARLNFCPLGSGPVAG
                     ATLALDRTIAARELDFTAPTANSMDATSDRDFALEFAQAASVLGVHLSRFAEELTLYS
                     SSEFGFLDLPEQFSTGSSAMPQKKNPDLTELLRGKTGRLLGAVTTLATLLKGLPLAYN
                     KDMQETQEPVFEVADTLAGILRILPHFTSALQFRHDRMLTATQSGFLNAMAAATYLVH
                     KGVPFRKAHEKIGNAVRYCLDKGCELGDLTLFELRQFGDEFDDDFFPAISIQATLDCH
                     DVIGGTARARVSAALAETQARVRALVARYAPTPAPEVPAHAGA"
BASE COUNT         1437 a         2618 c         1902 g         1296 t
ORIGIN      
        1 caacggctgg aaccagcacc ttgaactctg cagcagtgag ccctgtcgcc gtcatgggcg
       61 tctccggata cgccggcatg gagctggcgc gcctcctcct ccgccacccc gcacttgccg
      121 ccaaacctcc tctctttctc ggccgcgccg agagctcccc tgagccgctc gccgcaatcc
      181 atccccagct gatggacaac aacggaaccg gactcggcgc cggggccctc accatcgagc
      241 ccttctcctg ggaactcctg caatcgcgcg gcgtcaaagt tcttttcctg gccacacccc
      301 acgagcagtc ccgcgagtgg gtgccgcagg ccctcgagca tggtctccgc gtcatcgacc
      361 tcagcggagc gtggcgtctc acccatgctt cgaaccgcgc cgtttacaaa ttcgctgacg
      421 aagactcacc gattgcaacc catgtccagg cgaaggccgt ctacggcatg cccgaactcc
      481 atcgcgacga aattcgtgac gccggcgtgg tggcgaaccc cggctgctac gccacctcca
      541 tcatcctggc cgtcaaaccc ctcatcgccg ccggctgggt cgatctcgac cggggcatca
      601 tctgcgattc caagtccggt gtctccggtg cgggcaaagc tccgacagcg aagacccact
      661 tcatgcacgc tgccgacaat ctctccgcct acggcctctt ctcgcaccgg cataccggcg
      721 agctgcttga acagctggac gtcagcgcgg gccagatcgt cttcacgccg cacctcctgc
      781 cgatcccgcg cggaattctc tccaccatct atataggttt caccgaaccc caccagcccg
      841 atgaagtcga ggctctcttt cgggcgtttt tcgctcagag tccgcttgtc cgagtcttcc
      901 ctgccggcca gctgccccag atccagtact cggtccatac caactactgc gacatcgggt
      961 tccacctcgc cgcagacggc cgacgatgca tcatcgtctc ctgcctcgac aaccttctga
     1021 aaggcgccgc cggtcaggcg gtgcagaatt tcaatctcat gtccggcttg aaggaaaccg
     1081 acgggctcct ttgagatccg aagcgacggc catcaagagt acggcagaag ggttctgaaa
     1141 tatgagatac gtggtcaaac tcggcggtgt aacgctggag aatccggagt tgctgcacgg
     1201 ctgcgccaaa gccattgccg aactcgtaaa ggacggcaac caggtcgcga tcgtgcatgg
     1261 cggcggcgtc cagctcacgc gcacgctgaa gcagcttggc aagcagagcg agtttgtcgc
     1321 cggtctgcgc atcaccgatg ccgaaacccg cgacaccgcc ctcatggtcc tcggcggcgg
     1381 cgtcaacaaa gccctcgtag ccgccctggg ctcccacggc cagcccgccg tcggcctctc
     1441 gggcggcgat ggactcgtct tccgcgcgcg caagaagcgc accgccccgg atctcggctt
     1501 cgtgggcgaa attgctgcgg ccgatccgcg ctggctcgag gccatctggc agatgagcgc
     1561 tgtgcctgtc atctcttcga tcgcgcttgg cttcgacggc gaatactaca acatcaacgc
     1621 cgacgagatg gcggcggcct gcgccggcgc atgcaaagcc gacgctcttg tctttctgac
     1681 cgacgtgcca ggtgtgcttg cagccgacgg cagcgtcatg cgctggcttt cgattgatca
     1741 gattgccgaa ctcacccgcg atgccgttat caccggcggc atgctgccca aactgagtgc
     1801 ctgccgcgaa gccctgctca acggcgtgaa gcgcgtgcgc attctccccg ccaacgtcgc
     1861 cgaccttctg cccgacctct gcagcagccg cgtcaaccag ggaaccgagg tgatggtcgc
     1921 atgagcaaca ctctggcccc ggagttgcac ccaattgaga agaacaccat gaaacttgaa
     1981 gaactgcagg cagccgagtc cagactcctg cttggcacct acgaccgtta tcccctgctg
     2041 ttcgagcgcg gcgaaggcgc gcatctcatc gatgcgaacg gcgcccgcta cctcgacctg
     2101 ctcagcggca tcggcgtcaa cgctctcggc tacgcccatc ccgccatcga gaaagccatc
     2161 gccgaccaga gccgtaagct catccacatc tcaaacctct attaccacga aggccaggcc
     2221 gcccttgctt tgcgactcac cgaagccacg ggtatggatc gcgccttttt ctgcaatagc
     2281 ggaacagaag ccaacgaggc cgctctcaaa ctcgcccgcg ccagtgcgaa gctgcaacgc
     2341 gagaacggaa gatccctcgg cacgaaattt ctcgcgcttg agaacagctt ccatggccga
     2401 accatgggct cggtcgctgc cacgtggaag gacaagtacc gcgaaccctt caatcccgtc
     2461 atgcccggcg tcgaattcgt ccgcttcaat gacatcgatg acctgaaggc gaaattcacc
     2521 acggacgtct gcgccatcct cgtcgaagtc atccagggcg aaggcggcat ccggccggtg
     2581 tcgaaagaat tcctccaggc ggctcgcgac ctcacccact ccaccggcgc gctcctcatc
     2641 gccgatgaaa tccagtccgg catgggccgc accggcaaat tctgtgcgta ccagcactac
     2701 gggatccagc ccgacgtcac gacgctggcc aaaccactcg ctggtggcgt cccgctcggc
     2761 gcgctgctct gcaccgaaga agcctcgcag gccattcacg ccggtatgca cgggaccacc
     2821 ttcggcgggg gacccttagc atgcgccgtg gccctggccg tcctctccac catcgagcgt
     2881 gacaacctgc tccaacatgt aaccgagacc ggcacatatt tcgaggacaa gcttgctggc
     2941 ttggcgcaaa agcactccgc cattaccgaa gtccgcggcc tcggcctgat gctcgccgtc
     3001 gaactcgact ccgccgactt ggcaaagacc gtcctcggcg aaatgatgca gcgccgtatc
     3061 ctcatcaacc gcaccagcga gaccgtgctg cgcttcctgc ccccttacat ccttgagaaa
     3121 gcgcacgtag acgagagcat taccgctctc gacgagattc tgaccgaaca gacggcgcaa
     3181 actgccgcaa cctcaccctc aggaggaaag agaattggca actaaaacca tcgccgtatc
     3241 tgaacttgat tcggtcagcc ccgatgtttc gctcgcaggc cgcgatctct gttccgtcgc
     3301 agacctcacc cctgcagaac tagccggcat cctcgccctg gcgcacgacg tgaaggtgaa
     3361 tccgggtgcc tatcgctggg cactcgacgc aaagcagatg gtgatgttct ttgagaagca
     3421 gtctctgcgc acgcgcctta cctttcagac ggcgatgaat actctcggcg gctcggcgct
     3481 ttttgtcgac cagacttcat cgcccctcgg cgagcgggag tccctcgcgg acatcgcacg
     3541 caatgtcgaa cgctgggtgc agatcctcgt cctgcgcact tatgctcacg aaaccatcac
     3601 cgaaatggca cagtacgcca gcgttccggt cgttaacgcg ctcagcgatc tcgaacaccc
     3661 ctgtcaggcc atcgccgact tcatgactct ccaggagcgc ttcggcgatc tgaaaggctt
     3721 caacttcacc tacgtcggcg acggcaacaa cgtctgccac tcactaatgc tcaccgcagc
     3781 cttgctgggc gcccactgca ccatcggcac cccgaaaaat tacgccccta aagccgacat
     3841 cgtcgcccag gcgcgggcca ttgcgaacgt caccggctcc aacgttgctc tggtgaacga
     3901 cccggtcgcc gccgtccacg gtgccgacgc catctacacc gacgtctgca ccagcatggg
     3961 ctttgagcac gaagcaacca aacgcgcgcc catcttcaag ccgtatcagg tcaacgaggc
     4021 gttgatggcc caggcctcgc cgtctgccgt cttcatgcac tgcctgcccg cgcatcgcaa
     4081 tgccgaagtg accgacgccg tgctcgatgg cccgcagtcc gtggtttttg accaggccga
     4141 aaatcgcatg cacgcgcaga aagcgctgct tctgatgctg ttgggcgcgg gcaaaatgca
     4201 gagataactc gtcacaacag tggttacatt ctgaacgtgt aaagcgaata cttgtaacag
     4261 ggcacgtact ttgtcgtgtc gaggattttg cacgaaatgt ctgtcattct cgaaaatttg
     4321 cccgtaagcc aaaaagtcgg catcgctttt tccggtggcc tcgacaccag cgccgcgctg
     4381 cactggatga agcagaaggg tgccctgccc tgggcctaca ccgccaatct cgggcagcct
     4441 gacgaaaccg actacgacgc tatcccccgc aaagccctcg aatacggcgc ggaaaacgcc
     4501 cgcctcatcg actgccgtgg cccgctggtt cgcgaaggca tcgctgccct gcaatcgggc
     4561 gccttccaca tctcgacggc cggcgtaacg tatttcaaca ccactccaat cggacgcgcc
     4621 gtcaccggca ccatgctggt cacagctatg aaagaagacg gcgtcaacat ctggggcgac
     4681 ggctccacct tcaagggcaa cgacatcgag cgcttctatc gctatggtct gctcgtcaat
     4741 ccaagtttgc aggtgtacaa accctggctg gaccccgctt tcatcgacga gctcggcggc
     4801 cgggccgaaa tgtcggcctt cctgcaaaaa tccggcttcg actacaagat gtcagccgaa
     4861 aaggcatact ccaccgactc caatcttctc ggtgccaccc acgaagccaa agacctcgaa
     4921 cagctctcgt cgagcattcg gatcgtcgat cccatcatgg gcgttgcctc gtggcgcgac
     4981 gacgttgcgg tcaaacccga agaaatcacc gtccgcttcc accaaggctt tcccatcgca
     5041 ctgaacagca aggagttttc tgacccggtc gaattgctgc tcgaagccaa ccgcatcggc
     5101 gggcgccatg gcctcggcat gagcgaccag atcgagaacc gcatcatcga ggccaaatcc
     5161 cgcggcatct acgaggctcc gggcctcgcc ctgctcttca tcgcctacga gcgcctcatc
     5221 accggcattc ataacgaaga caccatcgag acgtaccgcg aaaacggccg caagctcggc
     5281 cgcctcctct accagggccg ctggttcgac ccccaggcca ttatgctccg cgaatccgcg
     5341 cagcgctggg tctcgagcgc cgtcacgggc caggtcacgc tggagttgcg ccgcggtaac
     5401 gactactcca tcctcaacac cgaatcgccc aacctcacct tccatcccga gcggctcagc
     5461 atggagaaga ccgactccgc cttttccccg cgcgaccgca tcgggcagct caccatgcgc
     5521 aacctcgaca tcaccgatac tcgcgccaaa ctcctgacct acgcgcaaag cggcctcatc
     5581 aatttgagca acggcgcaga aatgcctcag ctcaatggtg gcgcgacgaa gaacgaggaa
     5641 gcacgatgac cgaaccttcc tcctccgctc cgaccacaaa gatgtgggcg ggccgcttcc
     5701 gcgcgacacc cgatccccag ttcgaagcct ggcagcgctc cctccccttc gacggacggc
     5761 tgctgcccca ggaaatcgca gccagcaaag cgcacgctca caccatcgcc gtcgctggca
     5821 ttctgtctcc cgccgaactc gaaacgaccc ttcgtggtct cgaagccatt ctcgcgcgcc
     5881 tcgccccaca ggagccgggc agtcccttcc gggctacgat aacctcctcc caggccgagg
     5941 acatccacca cttcgtcgag ctcgctctca ccgaggagat cggcgacctc gccctcaagc
     6001 tccacactgg acgcagccgc aacgagcaga tcgccaccga cctgcgtctc ttcgttcgcg
     6061 acgccatcga cgcaaccctc gcccacctgg cgtcctgggc cagcgcgctt ctcgatcttg
     6121 ccgtcgagtc cgaaaacgcc gtcatgccct cctacaccca cctccagcgc gctgagcctg
     6181 tcctcgtcgc gcactggctc ctcgcctatg tggagatgct cctccgcgac atctcgcgct
     6241 tcgaggactg ccgcgcccgc ctcaacttct gcccgctcgg gtcgggtccc gtcgccggag
     6301 ccacgctcgc gctcgatcgc accatcgccg ctcgcgagct cgacttcacc gcacccacag
     6361 ccaacagcat ggacgcgacc agtgaccgcg acttcgccct cgaatttgca caagccgcct
     6421 ctgtgctcgg cgtccatctt tcgcgctttg ccgaggagct gaccctctac tcctcctccg
     6481 agttcggctt cctcgatttg cccgaacaat tctccaccgg ctccagcgcc atgccgcaga
     6541 agaagaatcc tgacctcacc gagctgttgc gaggcaaaac cggccgtctt ctgggcgccg
     6601 tcaccacgct cgccactctc ctcaaaggcc tgcctctcgc ttacaacaaa gacatgcagg
     6661 aaacgcagga gcctgtcttc gaagtggcgg acaccctggc aggcattctg cgcattttgc
     6721 ctcacttcac cagcgccctg caatttcgcc acgaccgcat gctcaccgcg acgcaatccg
     6781 gattcctcaa cgccatggcg gccgcgacct acctcgtcca caaaggcgtc cccttccgca
     6841 aagcacacga gaaaatcggc aacgcggtcc gctactgcct cgacaaaggc tgcgaactcg
     6901 gtgacctcac cctcttcgaa ctgcgtcagt tcggtgacga attcgacgac gacttcttcc
     6961 ccgccatcag cattcaggcc acactcgact gccatgacgt cattggtgga accgcgcgcg
     7021 cccgcgtgag cgcggcgctg gccgaaacgc aagctcgcgt tcgggctctt gtggcgcgtt
     7081 acgcgcccac gcccgcaccg gaggtgcccg cccatgctgg tgcgtaaagc gcgtctccag
     7141 gacgccacca acatctacga actggtcaac tcgctctccg gagacggcac cctcctccgc
     7201 cgccccttcg ccgagatctg cgaaaacgtt cgcgacttca ccgtcgctga atc
//
LOCUS       DASZYN010000118         4375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_712136, whole genome shotgun sequence.
ACCESSION   DASZYN010000118 DASZYN010000000
VERSION     DASZYN010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712136"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1392
                     /locus_tag="VHE33_03020"
     CDS             <1..1392
                     /locus_tag="VHE33_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96449.1"
                     /translation="NAGIRWEYGAPMSELYGRLVNLDIARGFTAVAPVLASDPKGTVT
                     GTKYPSTLLRPDYRGWQPRVGISWRPLPASTLVIRGGYGIYDDTSIYLGSAENMAQQA
                     PLSTSISVSNSAACPLTLANGFRNCAGTTANTYAVDPNLRVGYAQDWQLSMQKDFPWA
                     LVITATYLGIKGTRGTQEFLPNTYAPGGTDPYAGLPVGFIYRTSNGNSTRHAGELQVR
                     RRLRSGLTATLDYTWAKALDDDAQVGAQGHTATSEAAAPVGDFANNQPPPVTVAQNWL
                     NLAGERGLSTFDQRNVFKASFQYTTGMGLGGETLLGGWRGRFLKEWTLLTEITASSGT
                     PETPVYLAAIPGTGFTNILRPDVTGQALYAGAPGYHVNPGAFAAPPAGAWGNARRNSI
                     TGPDQFSLDTGMSRTFRLRSKWDLNVRAEASNLLNHAAWTSWNTTVNGTVFGLPTAAN
                     PMRSLLLTGRLRF"
     gene            1395..3581
                     /locus_tag="VHE33_03025"
     CDS             1395..3581
                     /locus_tag="VHE33_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVT96450.1"
                     /translation="MRRTIAAAMLLAVTATGALAQQTGSNEMAGQNGTFTLTVNSQLV
                     IETVTVKDKQGNFIPGLAAKDFTVTEDGQPQTIRIFEHQLMSDEVRPLAVTPHDQEQI
                     TLYKKLTRTQIAPESPGKPQYNNHRLLALYFDMTAMPPPDQLRALAAAEKFVRTQMTA
                     ADLVAVLRYQGGSVDVVQDFTADRNRLLSILETMIVGEGQGAVESIDDSSSADTGAAF
                     GQDDSEFNIFNNDRQLSALQTAAKMLGSLSEKKSLIYFASGMRMHGIDNLAQLHATID
                     AAVRAGVTFWPVDARGLVAGAPLGDASEGSPGNAGMYSGASAQAVSAQFQQSQDTLYS
                     LGADTGGKALFDNNDLTRGIVQAQRSIEDYYIVGYYTTNTAQNGRFRRVKITVDAPQA
                     DKLEYRQGYYANKTFNKFTDADKERQLEDALMLGDPVTELTIAMEIDYFQLNRAEYFV
                     PIIVKIPGRELALAKKFGAEHTLIDFVCEVKDDITGATMSNVRDFVNIKLSDQTAAEL
                     AHRPVEYDSGFTLFPGKYTIKFLARDDETGRIGTFQTTFTIPDLNKVTRRLAISSVVL
                     GSQKVALNDALYNASRGKEQAKADAANPLVQDGQKLIPSVTRVFNRDKEMYVYLQAYE
                     NDLATPQVVQASAAGQAATLPPPAVDASAPLFAWVTFYRDQKMVMETAPVAMTALPAT
                     RLGIVPFSFHIGLGSLAAGPYQCQISVLDPAGHRAAFWQGSVMLVH"
     gene            3746..4273
                     /locus_tag="VHE33_03030"
     CDS             3746..4273
                     /locus_tag="VHE33_03030"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HVT96451.1"
                     /translation="MTVAEELLSEFDEEFATTRKFLALIPNDKMLWRPHEKSMELGRL
                     AWHVADFPEWCLATLEQNPLRMTEADAQNYMHAWEGKTRQEIVAKFDTSLTKARAALQ
                     SIPDDKLTERWRMEWAGQTVIDEPRLNAVDKWTFRHMVHHRAQLGVYLRMLGVSIPGA
                     YGPSADEMEQVPAVA"
BASE COUNT          895 a         1346 c         1370 g          764 t
ORIGIN      
        1 aacgcgggca ttcgctggga gtacggcgcg cccatgtcgg agctttacgg ccggctggtc
       61 aacctcgaca tcgctcgggg atttaccgcg gtggctccgg tgctcgccag cgatccaaaa
      121 ggcacagtca caggcacaaa atatccgagc actctgctgc gccccgacta tcgcggatgg
      181 cagccgcgcg tgggcatctc gtggcggcct cttccggcat cgacgctggt gattcgcggc
      241 ggctacggca tctatgacga cacatcgatc tatctgggca gcgcggaaaa catggcgcag
      301 caggcgccgc tgtcgacaag tatcagcgtg tcgaacagtg ctgcgtgtcc gctgactctc
      361 gcgaatggat tccgtaattg cgccggcacc accgcgaaca cttatgctgt cgatccgaac
      421 ctgcgcgtgg gttatgcgca ggactggcag ctctccatgc agaaggattt tccgtgggct
      481 ctggtgatca ccgcgaccta cctcggcatc aagggcacgc gcggcacgca ggaatttttg
      541 ccgaacacct atgctccggg cggaacggat ccctacgccg gtctgccggt gggcttcatc
      601 tatcgcacct cgaacggcaa ctccacgcgc cacgcggggg agttgcaggt gcgacggcgg
      661 ttgcgcagcg gactgaccgc gacgctggac tacacatggg cgaaagcgct cgacgatgac
      721 gcgcaggtcg gcgcccaggg gcacacagca accagcgaag cggcggcgcc ggtcggcgac
      781 ttcgccaaca atcaaccgcc cccggtcacc gttgcccaga actggctgaa cctcgccggc
      841 gagcgcgggc tttccacctt cgatcagcgc aatgtgttca aagcgtcgtt ccagtacaca
      901 accggcatgg gtctgggcgg cgagacgctg ctggggggat ggagggggag gttcttgaag
      961 gaatggacgc tgctgaccga gatcacagca tcaagcggaa cgccggagac gcccgtctat
     1021 ctcgccgcta ttcccggcac cgggttcacg aacatcctcc gccccgacgt cacgggccag
     1081 gcgctgtatg caggggcacc ggggtatcac gtgaatccag gggccttcgc tgccccgccg
     1141 gccggcgcgt ggggcaacgc gcggcgcaac tccatcacgg ggcccgatca gttcagtctc
     1201 gacaccggca tgtcccgcac ctttcgtttg aggagcaaat gggacctcaa tgtgcgcgcc
     1261 gaggccagca atcttctcaa tcacgctgcg tggacttcgt ggaacaccac cgtgaacggc
     1321 actgtgttcg gcctgccgac tgcggcaaac ccgatgcgga gcctgctgct gacggggagg
     1381 ctgagattct agcgatgcga cggacgatag cagctgcgat gctccttgct gtcacagcaa
     1441 cgggtgcgct cgcacagcag accggcagca acgagatggc gggtcagaac ggcaccttca
     1501 cgctgaccgt caactcgcag ctggtgatcg aaaccgtcac cgtgaaagac aagcagggga
     1561 atttcattcc cggcctggcg gcgaaagact tcaccgtcac cgaggatgga cagccgcaaa
     1621 cgatcaggat tttcgagcat caactcatgt cggacgaagt gcggcctctg gctgtgacgc
     1681 cgcacgatca ggaacagatc actctctata agaaactgac acggacacaa attgcgcccg
     1741 agagcccggg caaaccgcag tacaacaacc atcgtctgct cgcgctctac ttcgatatga
     1801 ctgcgatgcc gccgccggat cagctgcgag cgctggcagc ggcggagaaa tttgttcgca
     1861 cgcagatgac cgcggccgat ctggtcgcag tcctgcgcta ccagggcggc tcggtggatg
     1921 tggtgcagga tttcaccgcc gaccgcaatc ggctgctcag cattctggag acgatgatcg
     1981 tcggcgaagg ccagggcgcg gtggagtcga tcgacgattc cagcagcgcc gacaccggcg
     2041 cggccttcgg ccaggatgac agcgaattca acatctttaa taacgaccgc cagctttcgg
     2101 cgctgcaaac cgcggcgaag atgctgggct ctctcagcga aaagaagtcg ctcatctatt
     2161 tcgccagcgg catgcgcatg catggcatcg acaaccttgc gcagctgcac gcgacgattg
     2221 atgcggccgt gcgcgccggc gtgactttct ggcccgtcga tgcgcgcggt ctggtggcgg
     2281 gcgcaccgct gggcgatgcg agcgagggat cgccgggcaa tgccggcatg tattcgggcg
     2341 cttcggcgca ggcggtgagc gcgcagttcc agcagtcgca ggacacgctg tattcgctcg
     2401 gcgccgacac ggggggcaaa gcgctgttcg ataataacga cctcacgcgc ggcatcgtgc
     2461 aggcacagcg ctccatcgag gactactaca tcgttggtta ctacacgacg aacactgcgc
     2521 aaaacggccg cttccgtcgc gtgaagatca ccgtcgacgc gccccaggcc gataagctcg
     2581 aataccgcca gggctattac gcgaacaaga cgttcaacaa attcaccgat gcggacaagg
     2641 agcggcagct cgaagatgcg ctcatgctgg gcgatccggt gaccgagctg acgatcgcca
     2701 tggagatcga ctacttccag ctgaatcgcg ccgagtactt cgtcccgatc atcgtgaaga
     2761 ttcctggccg cgaactggcg ctggcgaaaa agttcggcgc ggaacacacg ctcatcgact
     2821 ttgtctgcga ggtcaaggac gatatcaccg gcgcgaccat gagcaacgtt cgcgacttcg
     2881 tcaacatcaa gctcagcgat cagaccgctg ccgaactcgc gcatcggccg gtcgagtacg
     2941 actccggttt cactcttttt ccgggcaaat acaccatcaa gttcctcgcg cgcgacgatg
     3001 aaacgggccg catcgggacg tttcagacca cgtttacgat tccggacctt aataaggtga
     3061 cgcgccggct cgccatcagc tctgttgtgc tcggcagcca gaaggtcgcg ttgaacgatg
     3121 cgctgtacaa cgcatcgcgc ggcaaggaac aggccaaggc agacgcggcc aatcctctgg
     3181 tgcaggatgg tcagaagctg atccccagcg tgacccgggt ttttaatcgc gacaaggaga
     3241 tgtacgtcta cctgcaggcg tacgaaaatg acctggcgac gccccaggtc gtgcaggcgt
     3301 ccgctgcggg acaggcggcg acgctgccgc cgccggcggt cgacgcgagc gcaccgctgt
     3361 ttgcctgggt cacgttctat cgcgatcaga agatggtgat ggagactgcg cccgttgcta
     3421 tgactgcgct tccggcgaca cggctcggga tcgtgccctt cagcttccac attggcttgg
     3481 gctcgctggc ggctggaccg tatcaatgcc agatcagcgt gctcgatccg gctggacacc
     3541 gcgccgcctt ctggcaggga tcggtgatgc tggtgcacta gctcctgaac agccacagca
     3601 cagtgcgagc agcttcccag gcaatggcgg ccggagcggt acagggtccg attcgcggca
     3661 aattgatccg ctgaaaacca gacaaactcg gctcgttcgg gggccgggcg aggggtatac
     3721 tccgcggcaa acggaggtaa gaccgatgac cgttgccgaa gagctgctct ctgaattcga
     3781 cgaagaattc gccacgaccc gtaaattcct tgctttgatt cccaacgaca aaatgctctg
     3841 gaggccccac gagaagtcca tggaactcgg ccgccttgcc tggcacgtgg cggattttcc
     3901 cgaatggtgc ctggcgacgc tggaacagaa tccactgcgc atgaccgaag ccgatgccca
     3961 gaactacatg cacgcctggg aaggaaagac gcgccaggag atcgtcgcga agttcgacac
     4021 gtcgctgacg aaggcgcggg cggcgctgca atccataccc gacgacaaac tcaccgagcg
     4081 ctggaggatg gagtgggcag ggcagacggt gattgatgag ccgcgtctta acgctgtcga
     4141 taagtggacg ttccggcaca tggttcatca tcgcgcccaa ctcggcgttt atctgcgcat
     4201 gctgggcgtc tcgattcccg gcgcctatgg cccatccgcc gacgaaatgg agcaggttcc
     4261 cgcggttgcg tgagtccgtg gcgcgtcgcg gccacagcaa ggaggatgaa aatcaggatg
     4321 ttgaggaagt ggcgcgccct gagagattcg aactcccgac ctactgattc gtagt
//
LOCUS       DASZYN010000119         4536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_5364, whole genome shotgun sequence.
ACCESSION   DASZYN010000119 DASZYN010000000
VERSION     DASZYN010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5364"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VHE33_03035"
     CDS             complement(<1..212)
                     /locus_tag="VHE33_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HVT96452.1"
                     /translation="MAATMTKTALVRQLAEKLELTNKQSAAFLDLLADTAIKETKKSG
                     VFVIPGLGRLVKAERKARMGRNPQTG"
     gene            complement(544..1266)
                     /locus_tag="VHE33_03040"
     CDS             complement(544..1266)
                     /locus_tag="VHE33_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HVT96453.1"
                     /translation="MTKQPYFDEEALRFLRGLKRNNKREWFEKRRAVYEEKLKAPMLA
                     LIERLTDGMADYAPAHMRPASKCMFRIYRDTRFSSDKSPYKTHLGAWWARNGLEKTSG
                     GGYYMHIGAEEFVIAAGVYMPEKNQLLAIRRRLQEHHEEWKKLIEDKKLLRVFQVHDP
                     MALTRPPKGFPPEHPAMVWIKWRQWGVTTHLPAEAALKANVAEEIEKRFRLAAPLVDF
                     LNAPLVRAERPRPAWAGGRLHG"
     gene            1379..1792
                     /locus_tag="VHE33_03045"
     CDS             1379..1792
                     /locus_tag="VHE33_03045"
                     /inference="COORDINATES: protein motif:HMM:NF044509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector immunity protein Tgi2PP"
                     /protein_id="HVT96454.1"
                     /translation="MLSLAAVAAAAQSARHQITAHQAHQIAVLVAHHDDIDVSDTHIE
                     LNSMDLGRDFTPGFYSFILIRESTSPGPDETLRRYAISRRTGDVWEMNLCTHYSFPEL
                     AHLQHSFLGRAANAAEISAQSRRLGCSKQRAAPTS"
     gene            complement(1799..2359)
                     /locus_tag="VHE33_03050"
     CDS             complement(1799..2359)
                     /locus_tag="VHE33_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HVT96455.1"
                     /translation="MNDLGAAPNLLTFLRLCIVPFLVLAVLDHRFEMAFYLFVLAGVT
                     DALDGLLARWLHQRTMLGQYLDPVVDKLLLSTLFLVLHHEALISRRVTVLVFARDLGI
                     LVVAAILYATTSIRSFKPSAIGKTNTMAQILAVAAVMLAEFFGPPWVLDLRSWALTAT
                     VILTLLSGFHYAWRVVRQIGSPGVAG"
     gene            2575..2651
                     /locus_tag="VHE33_03055"
     tRNA            2575..2651
                     /locus_tag="VHE33_03055"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2609..2611,aa:Val,seq:gac)
     gene            complement(3356..4420)
                     /locus_tag="VHE33_03060"
     CDS             complement(3356..4420)
                     /locus_tag="VHE33_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HVT96456.1"
                     /translation="MRSEDEQQGEVFSYVGLEERVPKDHPLRAIRQMIDAALKSLDGE
                     FSRLYARCGRPSIPPERLLRAQLLQVLYSIRSERQLMEQMDYNLLFRWFVGLNMDDRV
                     WDVTVFTKNRERLMAGGISQQLLVAVVGQARANRLLSEEHFTVDGTLLQAWASRRSFV
                     PKKDPPSRGTGARGRKLLRDTHESKTDPQARLYRKSGSDAVVPVYLAHVLTENRHGLI
                     VGAMVTQAGNAAEREAALHLLDRYRPGRGSTLGADKSYQREEFIAGLRERGIRPHVAE
                     HEPSRNWPNWLREQERCDPGLAVSQQRRKLVEKVFGWAKQDRIRQVKLRGRARVDWLF
                     QLVAAAHNLLRMQKLIPLQA"
BASE COUNT          823 a         1358 c         1401 g          954 t
ORIGIN      
        1 tcgccggtct ggggattgcg ccccatgcgg gctttccgct ctgccttcac cagacggccg
       61 agtccgggaa tgacaaaaac gccgctcttc ttggtctctt tgatcgctgt gtccgccagc
      121 aaatccagga atgcggccga ctgcttgttc gtgagttcca gcttttccgc caactggcga
      181 accagtgcgg ttttggtcat tgttgctgcc attgagtctt cctccttgtt ttttcttctt
      241 cgaggtcgcc ggagtaactt cgttccaacc tgtgcgaacg ccagtattga tgcggctccg
      301 aaagaacctg ccgccgttaa ccttgcggct ttcagcagcg tttgtcaatg gaaaaaccca
      361 tgtttccaca ggttttttgc ggttttcttt ggtttttatc tggaaaaggc cgattttaag
      421 cggtttttgt gacctggaat gcttccggac cgcgattgac gggcctttgg agattccttc
      481 gcggcagtcg gggtggcttg cgctgcagtc tattcctttc tcgccacaag cggcaacgcg
      541 cgctcagccg tgcagccttc cgcctgccca cgccgggcgc ggccgctctg cgcgcaccag
      601 cggcgcattg agaaaatcga ccagcggagc ggcgaggcga aacctctttt caatctcttc
      661 ggcaacgttt gccttgagcg cggcctcagc gggcagatgg gtggtgacgc cccactggcg
      721 ccacttgatc cacaccattg cgggatgctc ggggggaaat cctttgggcg ggcgcgtgag
      781 tgccatggga tcgtgcacct gaaaaacgcg cagcagcttc ttgtcctcga tcagcttctt
      841 ccactcctcg tgatgctcct gcagacggcg ccgaatcgcc agcaactgat tcttctcggg
      901 catgtagaca ccggcggcga tgacaaattc ctccgcaccg atgtgcatgt agtagccgcc
      961 gcctgaggtc ttctccagac cattgcgagc ccaccacgca ccgagatgcg ttttgtaagg
     1021 cgatttgtcg gaggagaacc gggtgtcgcg ataaatacgg aacatgcact tcgaagccgg
     1081 gcgcatgtgt gcgggcgcat agtcggccat gccgtcggtc agacgctcaa tgagggccag
     1141 catgggggcc ttcagtttct cctcgtagac cgcgcgccgc ttctcgaacc actcgcgttt
     1201 gttgttgcgc ttcaggccgc ggagaaagcg cagggcctct tcgtcaaagt atggctgctt
     1261 cgtcaccggc tcattttatc ggcgatgggc tgtggattca ccgcgagttg tattgcgccg
     1321 ggacggctgc acgcttcaca ctgagagtat gcagcgcagg ttccgaggac ttgtggcgat
     1381 gctctcgctg gctgcggtgg cggccgcggc gcagtctgcg agacaccaga tcaccgcgca
     1441 tcaggcgcac cagatcgccg tactcgtcgc gcatcacgac gacatcgatg tatcggatac
     1501 acacatcgag ctgaacagca tggacctggg acgcgacttc acgccggggt tttacagctt
     1561 catcctgatt cgggagagca cgtctccagg gccggatgaa acgctgcgcc ggtatgccat
     1621 cagccggagg accggcgatg tgtgggagat gaatctgtgc acgcattaca gcttcccgga
     1681 gctggcgcat ctgcagcatt cattcctggg gcgtgctgcg aatgctgcgg agatctctgc
     1741 acagagccgg cgcctgggct gctcgaaaca gcgagcggct ccgacctcgt gagccgggct
     1801 atcctgccac gcctggagat ccgatctggc gtacgacgcg ccacgcgtag tggaagccgg
     1861 agagcagcgt aaggatcacg gtggcggtga gcgcccatga gcgcaggtca aggacccacg
     1921 gcggaccgaa gaattcggcg agcatcacag cggcgacggc gagaatctgc gccatggtat
     1981 ttgtcttgcc gatggcgctg ggcttgaagc tgcggatgga cgtcgttgcg tagagaatgg
     2041 cggctacaac gaggatgccg agatcgcgcg cgaaaaccag cacggtgaca cggcgcgaga
     2101 tgagcgcttc atgatgcagc accaggaaca gggtgctgag cagaagcttg tcgacgaccg
     2161 gatcgagata ctgaccgagc atggtgcgct ggtgcagcca gcgtgccagc agtccatcga
     2221 gggcatcggt gacaccggcc aggacaaaca ggtagaaggc catctcgaag cggtgatcga
     2281 gaacggcaag caccaggaac ggcacaatgc agagccggag gaaggtcagc agattgggag
     2341 cagccccgag atcgttcatc gcatggtttc gccgtgccgg ccggatcgtt cgcgcgccgc
     2401 aacggatcgg tggaggaagg attcatggta tcgcagcgag ggcgaatttc ccacggtggg
     2461 gtgagccgtg ggggcactgg agtggaggag agcggggcgg gagggttggg tgtggagggc
     2521 gttcgagcgg cgcggcattt tgctatcatg agcgaaggcg attcagcctg gtgtaggcgc
     2581 gtagctcagt tggttagagc gctaccttga cacggtagag gtcaggagtt cgagtctcct
     2641 cgtgcctacc attcttttct tctcaatctg gtgttgcgga tcggcgaagt tctcgtttgc
     2701 cgttcctgcg cgtcgcgaga gcgaatcttc ccttcctcca tttccacacg cgtccttcga
     2761 tttgcacaca aatattccgc gggagcgcgc gggctcgcgt gccataatcc tgatcaacgg
     2821 gaagagcaat cttccttacg aagagagcag gttcgttgca acgctcggcc ggcaacggcg
     2881 gagcaggctt gcgggaaacc ggaagtttca gcgaactgga agcttcgcag tcatggtctc
     2941 aaagaggtcg ggttcggcgg ggtcaacctc gcagactcct gttgccgttg agactgagtc
     3001 tgatctggca gccgaagtcc aggtcttcgc aagttcggac agagggggcg agattccttc
     3061 agggagtctc gccctttcgc ttttgggggg atggagggtc gggggctaat gtggtgtccc
     3121 gtaacttctc cgaaacagtt gaggatcaga ggtgaacgaa gagaaagcct caggggctga
     3181 agcccacatc ctttttgcgc gttttgcggc acgactgaac agcttgcgga aaaagtgagt
     3241 gatttcgggc tgtccggctg gaggcgttgt tgtttgagac aggaaggatg ttgggcggag
     3301 gtcctgtttt gcctgtgcga tgcggctaac cccggcgctc agacatttca cctcctcagg
     3361 cctgcaacgg aatcagcttt tgcatgcgca gcagattgtg cgcggctgcg accagctgaa
     3421 acagccagtc cacgcgcgct ctgccgcgca gcttgacctg ccgaatgcgg tcctgttttg
     3481 cccagccaaa caccttctcc accagtttgc gtcgctgctg gctcacggcc aggcctggat
     3541 cgcagcgctc ctgttcgcgc agccagttgg gccagttccg gctcggctcg tgctcggcaa
     3601 cgtgcggccg gatgccgcgt tcgcgcagcc ccgcgataaa ctcctcgcgc tgataactct
     3661 tgtctgcgcc cagcgtgctg cctctccccg gccgatatcg atccagcagg tgcagcgccg
     3721 cctcgcgttc ggctgcgttg cctgcctgcg tcaccatcgc tcccacgatc agcccatgac
     3781 ggttctcggt cagcacgtga gccagataga ccggcaccac tgcgtcgctt ccgctcttgc
     3841 ggtacagccg cgcctgcggg tcggtcttcg actcatgggt atcgcgcagc aacttccgcc
     3901 cgcgcgcccc tgtgccccgg ctgggtggat cctttttagg aacaaaactt ctccggctgg
     3961 cccacgcctg cagcagcgtt ccatccacgg tgaagtgctc ttcgctgagc aacctgttgg
     4021 cacgcgcctg tcccaccacg gccaccagca gttgctgcga gatgcctccg gccatgagcc
     4081 gctcccggtt tttggtgaac acggtcacgt cccacacccg gtcgtccatg ttcagcccca
     4141 cgaaccagcg aaacagcagg ttgtaatcca tctgctccat cagctgccgc tcggaacgaa
     4201 tcgaatagag cacctgcagc agctgcgccc gcagcagtcg ttccggcggg atcgacggcc
     4261 gtccgcaacg cgcatacagc cgcgaaaact ccccatccag cgacttcaga gccgcgtcga
     4321 tcatctgccg gatcgcccgc agcggatgat ccttcgggac tcgctcttcc agtcccacat
     4381 agctgaacac ctcgccctgc tgctcgtctt cgctccgcat gcttctctac taccaggttc
     4441 ctctgtgtac ttccagtccc tgtgcagata ctttttccgc aagctgtgaa gtcgggcgct
     4501 gacgcgccag cggcccgatt gtgatcggac gtagcg
//
LOCUS       DASZYN010000120        19736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1033027, whole genome shotgun sequence.
ACCESSION   DASZYN010000120 DASZYN010000000
VERSION     DASZYN010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 19736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19736
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033027"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..644
                     /locus_tag="VHE33_03065"
     CDS             153..644
                     /locus_tag="VHE33_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HVT96457.1"
                     /translation="MAITRWDPFRDVLALQNRMNSLFQDFNRGQSEGEAAFTTAAFVP
                     PVDIYEDEHKIVLKLEVPGMKENELDIQLENNVLTVKGERKFEKEEKEENFHRIERRY
                     GSFYRSFTIPNTVNPEGVKANYDAGVLRVELEKRAEAKPKQIKVAVGGPSLEAGKGVK
                     SAA"
     gene            775..3432
                     /gene="clpB"
                     /locus_tag="VHE33_03070"
     CDS             775..3432
                     /gene="clpB"
                     /locus_tag="VHE33_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HVT96458.1"
                     /translation="MAIRWDKFTVKSQEAVQSASQLAAENGNPEILPLHLLAVLLGDK
                     EGVIPAVLDKIGVPVGQLDARAQEAVNQLPKVSGASAQPGASNALNQVFETAFKEAEQ
                     FKDDYVSTEHLLLALTRLKNDGAQLLLASLGADHDAVLKSLAAVRGNQRVTDQNPEAK
                     YQALEKYAKDLTELARRGKLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAI
                     VEGLARRIVHGDVPDILRDKRVISLDLGSMLAGAKYRGEFEDRLKAVLKEIEESNGQI
                     ILFIDELHTLVGAGAAEGAIDASNMLKPPLARGELRAIGATTLNEYKKYIEKDAALER
                     RFQIVYVGEPNVEDTIAILRGLKERYEAHHKVRIRDSAIVAAATLSHRYISDRFLPDK
                     AIDLVDEAAASLAIQIGSVPQEIDELEREATSLEIERAALKRENDANSRERLEHVERE
                     VAQLREKATALRARWTKEREAITRLSDLKKQIETLRFEADEQTRRGNLERAAQIQYGE
                     LPKLETEMKKLDAAQDGVAASSQRMLKEEVDEEDIAKIVSKWTGIPVSRMLEGEVKKL
                     VRMEERLKERVVGQDAALSVVANAIRRSRAGLSDPKRPIGSFIFLGPTGVGKTETARA
                     LAEFLFDDEQSMVRLDMSEYMEKHAVARMIGAPPGYVGYDEGGQLTEAVRRRPYSVIL
                     LDEIEKAHPDVFNVLLQVLDDGRLTDSKGRTVDFRNTVLIMTSNLGAGFLTGDALKDE
                     TTFGHATESVMKVLREHFRPEFLNRVDDVVIFRPLGEQQLQHIVDLRLKDLEKLLADR
                     HITIELTDAARHAIFAAGYDRAYGARPLKRAIQRMIQDPLAMKILDGEVLHGDHVRID
                     AHKGSSALQFEVVSRAGIPLEHGVAIGQA"
     gene            complement(3520..3936)
                     /gene="mscL"
                     /locus_tag="VHE33_03075"
     CDS             complement(3520..3936)
                     /gene="mscL"
                     /locus_tag="VHE33_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HVT96459.1"
                     /translation="MLKGFRDFILRGNVMDLAVAVVIGAAFTAIVNALVTDIVNPLIA
                     AMVHQPNFSALIFDVHGGKIKYGEFLNALISFVLIAGVVYFIFVLPMNALLKKFHPAK
                     VAPPTTHPCPECLADIPLAAKRCSHCGQPVAPAAAA"
     gene            complement(4520..5482)
                     /locus_tag="VHE33_03080"
     CDS             complement(4520..5482)
                     /locus_tag="VHE33_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HVT96460.1"
                     /translation="MTRSPILLLHGGAWDIPADQLDAHEQGVFDALQEGWRILERGGS
                     SLDAVEAAVASMENDPAFDAGRGSFLTRDGRVQLDALIMDGATLRAGGVACVERIRNP
                     IQAARLVLDKSPHVYFVGPGAEAFAQAQGMTLIDNAELVLDRERQRLAEAQAREQSGA
                     PDRTFSGDNVSNQDSHDTVGAVALDAEGRIAAGTSTGGTLNKDPGRVGDSSLIGCGCY
                     ADNASAAVSLTGWGEPIMKLVLGKWATDRVLAGRSPEQVAPEAMAYLLRHAGGHGGII
                     LLAPDGRYGLAHNTPRMAWGVHTADGPRTGTLVREQDENETDND"
     gene            5559..5927
                     /locus_tag="VHE33_03085"
     CDS             5559..5927
                     /locus_tag="VHE33_03085"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HVT96461.1"
                     /translation="MLSQKRITVYHMAARETSASDAEIVLPGRGLQYEGKISYLTTEG
                     CLIDTRCRLEPGTVVEVWMRTEGMPLRVLANLVERREQGVEFHFHEMTNRKMDQIEIL
                     RGELAEEAARNEIGQQSRGA"
     gene            complement(5950..7395)
                     /locus_tag="VHE33_03090"
     CDS             complement(5950..7395)
                     /locus_tag="VHE33_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96462.1"
                     /translation="MGPLSVYFRCPGDFLEGVRVAAPAAAAPAGYFAFGPETTLYGRL
                     APQVPAASPEEPLHDASADVAITDGAIGLPFDPREAIANLRHEAYTGGDARSGTQSLA
                     AKIYYFFRPLLSVAARKHLQKLRLRNWNRRPFPRWPVDFSVDNLHEQLLVLALRASGI
                     QHVPFIWFWPEGHSACAMMSHDIETEAGRDFCATLMDLDDSRGIKGSFTVIPEERYEV
                     PQTLIDSIHDRGHEVAVHDLNHDGHLYDNRAQFLERVSKINEYGRKFGASGFRAGVLY
                     RNQDWFNEFQFAYDMSVPNVAHLDPQRGGCCTVMPYFIGDLLEIPLTDIQDYSLFHIL
                     NEYSTDIWKQQTAMILARHGLMSFIVHPDYIIEPEARATFEALLDHLAHLRDTENVWT
                     ALPREINQWWRQRAAMTLVRAKDGWRIEGAGAERARIAWAREIDGKLFYSVEPAATAA
                     LSPSEKFHLLSGFRPLPGRVPVTDDSSAEIA"
     gene            7755..11393
                     /locus_tag="VHE33_03095"
     CDS             7755..11393
                     /locus_tag="VHE33_03095"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT96463.1"
                     /translation="MPTQFGSATAVSSSADDLRALETIVPASKESRADETLVPPSKSD
                     AATMVPPSRSDAVTVVPGSSDPAGGSGGGSRTGGSAGWTSETAELEPGTVLAGRYEIR
                     AVLGTGGMGSVYRAHDRELDRLVALKVIRPELARNPAIVDRFKQELRLSHKVTHRNVV
                     RMYDLSEDAGMRFVTMELVSGRDLRTVLEERGKLPTDEAATVLEQICFALDAAHSQGI
                     LHRDLKPQNVMVEENGRVVVMDFGLARTIEGDGMTQSGAMVGTMEYMSPEQALGKELD
                     QRSDIFALGLIGYEMLTGAMPFKAESAIASLLKRTRESAAPLMKVDSSIPAVLSGIIE
                     KCLTVDVEQRYKSVGEVLHDLEVWRGKAAAASLHFKADVPSTGLSGRWLFITGGGALL
                     IALVAGISLGVRHFSGSQTTATPASAPSISLAIMPFYNASGDSSMDWLGASLAEMLST
                     DIGGSSAVRMVSPDRLQQVLGDLHLTANSQADASTLKRVAEFTNAQTIVYGQFLKAGN
                     TIRISSTVVDLAHDGVRSTVTTDVPDQNQLLASVDKLAGQLREKLTADPNVLNDLKAH
                     AVRPSTTSVEALHDYENGLALERAGDNLRAIEAFHAATEADTNFATGFAHLAQAYSSQ
                     GQDDLAQSASRTAMELAANLPAGERYRIEASNAEINNNTEKAIQAYQQLAAANPSDTD
                     VQFALARLYEQNGDYAEARQHLAAVLAGDPKNVAALLASGRVAIRGGDAKGGLDFLSK
                     ALPIAVELNNQQQKAAILQATGIAYSLMGRPDDALNNFQQSLAIKQQIGDKRGAAASL
                     EQVGSLQAAAGKADAALKSYQQALSLRKELGDQSGIGNTLIDLGSFYGNHGKSDQALQ
                     YYTDALQVERQLGDVANQALCLNNIGSIKVDQGHYQDALTYLEQSYDLRQKLNVPADI
                     GESLHDLAEVNTKLGQYDTALSYYLKAIAAYRSANDQHGVAMASDAMARLFAAQGRYG
                     AALHSMQDAVSILRQSKEMTWLTVEAIGGWGDLLAQVGRAPEGKASLEEALNDAHQIK
                     DDAYVAIATNLMGDYYYYQGDYPNARAEYAQAQTIAAKTTDRETQLRSKMNVAKTDLA
                     LGRAAAAVPVLKKLTQDADALGLKAEAVECSIYLTEALMATKNDAAAGQTLTLAQARA
                     ENLGLRILQVKADSLAAALAAKSGRTAEAQRNRQQASRILDAIVKEDGAARAAQRADL
                     KQLAASTQ"
     gene            complement(11584..14367)
                     /locus_tag="VHE33_03100"
     CDS             complement(11584..14367)
                     /locus_tag="VHE33_03100"
                     /inference="COORDINATES: protein motif:HMM:NF037833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HVT96464.1"
                     /translation="MKLASAKALHVVVCAAATLFALLPVRASAQQITYYNFDTPATAS
                     PLQYSYTCSAANPSANPLFCLNDGTGTMSNPLFIQDPVGSGTWATQMTPDAASQAGSL
                     WFSVPQVVSGGFNAWFEFKLTPDNTGYSYATADGIAFVIQNAHGGGAVDPSSGCQETG
                     SGPTVLGGGGGCLGYSGIDNSVALEFDTYFNSFDPDEAAHGNFNDDNHIALQDCGAGQ
                     VNSAAHIASDSGPACEVYLPGPDETQIPTLVSNPNASLVANAAPVPVVLSDGNVHQVV
                     VVYNGPSDSPANYLYVYLDPAFDPGTVTPVAGSVPLFSGPYDITQALNLLDTGSGPNS
                     AYIGFTSATGAAFEQNELVGWTFTPHTPVTQQQPLQPAGSPTYTQFPYGTHTYGVQYP
                     GDGPATTGTSMTVTANTVSPTLFSQLIAGTPFQGSACQVYDDTGGNCIIYSVSCFVTG
                     HPDQVVACPAVTSVPNCIGANAASCINVKTVFNSSVTPVSPGYLQGDPFFSQISALNV
                     SGDTANFTCTGECSVTIGQTVSVIGATPAGFNGSYTVTSVSAPNAFVAQTAVGASGSA
                     TTAGYLTSNNLQNIFVSYTPANIDGTSKGTTLNFSDFVFTSVTNNATTQTQLSAASMT
                     PTLGQPDLLTATVAGSATQIAPPTGNVLFYAGATLLCSSPVSTTAGVTTATCSYTPTT
                     TGPSTITAQYLGDTAHLLSNATPLILTIPVGQMLNINPSSIDFGTLYLGSIVTKIVTV
                     TNTGTGAVTIHDPLIAIVKGGNSKEFVTLNLCPKSLAAGKSCVMTVTFIAGPYYTPQT
                     ATLTINDSAAGSPHTVPLTATVINPIAKFSTGSLNFGKVKTGTTSSPQSVTITNTGAT
                     SLTISNVSVSGGNAADFHPTNGCTAPLAPKGTCSIGVTFKPAAKGSRSATLIVTDNAF
                     NSPQAISLSGTGN"
     gene            14672..16708
                     /locus_tag="VHE33_03105"
     CDS             14672..16708
                     /locus_tag="VHE33_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT96465.1"
                     /translation="MTSSALQNGRFSVALVGLGLLLALPSLARAQAADQDLMAMPLED
                     LARVQVYSASRHSEDVRKAPSSVSIITSEDIRRYGWRTLGEALRSLRGFYTAYDRQYT
                     YLGIRGFLRPGDFNSRILLLVNGHRYNEDVYDSASFDSEYPIDLNLVDHIEVVRGPGS
                     SLYGTNAVFGVINVITRQPGSETVAEVAGTTGTFLQRTARATLQGRVGGVSGLLSATL
                     DRNPGAPVLFFPEFAVPETNNGYAESSDGGSMQQGFADLAWGDFRVQGMFGNRVKVIP
                     TASYGMVFNDPADSDQDTRAYVDVSDRRGVGKGRTLNLRAYYDVYNYVGRGDYAEPGN
                     EAPVRIYGKARADWVGTEATLTWQAGTQTVIAGASYEQDIHVTQRSVAAELPDMLHIE
                     ESPWLVAAYGEMELHMLPKTILHAGGRLDHYSTFGAAFSPRLAAIWTPGDRTSVKYIL
                     AGAFRAPSSYEEFYADGMTIEPASRPLQPERIRSNELVFERSLAKGVRLTVDGYDDRL
                     EKLIDQSPDLATGLTFFTNVGRISSRGLEVELEAERPSGWSGHATYTATATTNDLLGR
                     PLENSPITQVKVASSLALGRWVTGSAEVMYVGPMRDYRQGRLASYVLPDVTFASRPLR
                     NGWQFSSSLYNVFDRRWYSPAGPNDPQSQIQMDGRSWRIQVGYRWPMRGGRREQ"
     gene            16705..17274
                     /locus_tag="VHE33_03110"
     CDS             16705..17274
                     /locus_tag="VHE33_03110"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HVT96466.1"
                     /translation="MKHVAVWRCLAWGLVFWLPGAGPVSSSTMRAQSRDERAVRAAYV
                     YNLIKYVEWPAPGKDLTIALVGDAGTGEVISQLLNGRTREGQTLRVVQPVSVEALQGC
                     SVVYVSAAPESDIRKILERVKGRPILTVGESDGFARAGGMVALVNTGDHIQIEVNLEA
                     VQAGGIRISSRVLGLATIVHSAAKGGDGR"
     gene            17271..19346
                     /locus_tag="VHE33_03115"
     CDS             17271..19346
                     /locus_tag="VHE33_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVT96467.1"
                     /translation="MSPPVRASFAHRMTILALLASSVSSATLMAAFLGFDSVSARRQL
                     ESRLSILTNIVGQNSAAALLFDDRAAAMEVLGALDAERSVVSACLYGVSGRLFAEYQR
                     EKAPQSCPEQFVPAGGTARGFVTMSRPVERGGDAAGTLVLRADVKEIVERWRQMLEIT
                     ALLLLGALAAGGIAGSLLQRRISQPVQALAAAMHEVTERHNFGARVATEGNDEIARLG
                     GGFNTMLAELEKRAAEKTAFEAQLQRQAMNDDLTGLPNRRLLADRLTHALDLARRRGY
                     EVALLYIDLDGFKLVNDSLGHAVGDQLLRQVAERLRSRVRSSDTLARLGGDEFAVVVS
                     GEKVREQGSVAAMALLAVLAPSFSVEEHEITISASIGMSLFPENGSTPIELLQQADGA
                     MYAAKRMGKNRLMAFSDELGASIRERLKLETELRHAIAQGEIRAYYQPEFDVNTGELM
                     RFEALARWIHPTLGFISPGQFIPIAEETGLITALGQHMMEIACREAMEWQEIAGRAVQ
                     VAVNVSSLQLMRETFVEEVAEILRKTGLDAHRLQIELTESVTVNGAAQVGAAMGELAE
                     LGVSLAIDDFGTGYSCLSYLPKLPFDALKIDRSFVSELGIRREVDAMVHSLVTLAHNF
                     GMRVIAEGVETEEQMALITELGCNEVQGYLLGKPTSDPRACLASAVGNRRCGDKITAD
                     TKILTSETI"
     gene            complement(19363..>19736)
                     /locus_tag="VHE33_03120"
     CDS             complement(19363..>19736)
                     /locus_tag="VHE33_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HVT96468.1"
                     /translation="HAFGDEIVLTAFLIFGIFAITEQYNEMAPGANFGALMIGFLVAL
                     IGASMGYLEAWAINPARDFGPRLFCFFAGWGGSALPSPQSYWWVPILGPLLGGAVGGG
                     AYQLLIHPFLPARLRASARGN"
BASE COUNT         4068 a         5770 c         6305 g         3593 t
ORIGIN      
        1 ctctttttca atattgacaa taaaacactc atattttctg catcgcttgt gctatagtgg
       61 catcagatgt tgtggacggc gggtctgctg gagccctcga aggcacccgg acccggccca
      121 actctcaaaa ttctgcttgg aggattttcg caatggctat cactcgttgg gatccctttc
      181 gtgacgtact cgctctccag aaccgcatga actccctctt ccaggatttc aatcgcggtc
      241 agagcgaagg cgaagcggct ttcacgaccg cggcgtttgt tcccccggtc gatatttacg
      301 aggacgagca caagatcgtc ctgaagctcg aagtacccgg aatgaaggaa aacgagctgg
      361 acattcagct cgagaacaac gtgctcaccg tgaagggcga gcgcaagttc gaaaaggaag
      421 agaaggaaga gaacttccac cggatcgagc ggcgctacgg cagcttctat cgctcgttca
      481 cgattccgaa cactgtgaac cccgaaggcg tgaaggccaa ctacgatgcc ggcgtcctgc
      541 gggtggagct ggaaaagcgc gccgaagcga agccaaagca gatcaaggtc gccgtcggcg
      601 gtcccagcct cgaagcaggc aagggcgtga aatcggcagc ttgataagaa gcaagccagc
      661 tttgcaatcc gatggggttg cgcgcaatat cgagcgggag ggagctgtct tcctcccgcg
      721 taattttatg aggtcactga tcgctgacaa cggatcactg acaactgagg ttttatggca
      781 attcgttggg acaaatttac ggtgaagtcg caggaggcgg tgcagagcgc cagccagctt
      841 gcggcggaga acggcaatcc cgagattctg ccgctgcatt tgctggcggt gctgctcggc
      901 gacaaagagg gtgtgatccc ggccgtcctc gacaagatcg gcgtgcccgt ggggcagctt
      961 gacgcgcgcg cgcaggaggc agtgaatcag ctgcccaaag tcagcggcgc atcggcgcag
     1021 cccggcgcca gcaacgcgct gaatcaggtc ttcgaaacgg ccttcaaaga ggccgagcag
     1081 tttaaggatg actacgtttc gaccgaacat ctgctgctgg cgctcacgcg cctcaagaat
     1141 gacggcgcgc agctgttgct cgcctcgctc ggcgccgatc acgacgccgt cctgaagagc
     1201 ctcgccgccg tgcgtggcaa tcagcgcgtg acggatcaga atcccgaggc gaaataccag
     1261 gccctggaga agtacgccaa ggatctgacc gaactggccc gccgcggcaa gcttgatccg
     1321 gtcatcggcc gcgatgaaga aatccgccgc gtgatccagg tgctttcccg ccgcaccaag
     1381 aacaatcccg tgctcatcgg cgagcccggc gtcggcaaaa cagccatcgt cgaggggctc
     1441 gctcgccgca tcgtgcacgg cgacgtgccc gacatcctgc gcgataagcg cgtcatctcg
     1501 ctcgatctcg gctccatgct cgccggcgcc aagtatcgtg gtgagttcga ggaccggctc
     1561 aaggccgtcc tcaaagaaat cgaagagtcg aacgggcaga tcatcctctt catcgacgaa
     1621 ctgcacacgc tcgtcggcgc cggcgcggcc gagggtgcga ttgatgcctc caacatgctc
     1681 aagccacccc tggcacgcgg cgaactccgg gccatcggcg cgacgacgct caacgagtac
     1741 aagaagtaca tcgagaagga cgccgcgctc gagcggcgct tccagatcgt ctacgtcggt
     1801 gaaccgaatg tcgaagacac gatcgcgatc ctccgcggcc tgaaggagcg ttacgaggcg
     1861 caccacaagg tccgcatccg cgactcggcc atcgtcgcgg cagccacgct ttcgcatcgc
     1921 tacatctccg accgcttcct gccggacaag gcgatcgacc tggtggacga ggccgccgct
     1981 tcgctcgcca ttcagatcgg ctccgttccc caggagattg acgagctgga gcgcgaggcc
     2041 acgtcgctcg aaatcgagcg cgcagccctg aagcgcgaga acgatgccaa ctcaagggag
     2101 cgcctcgagc acgtcgagcg cgaagtcgca cagcttcgcg aaaaagccac cgcccttcga
     2161 gcccgctgga caaaggagcg cgaggcaatc actcgcctga gcgacctgaa gaaacagatc
     2221 gaaacgctgc gtttcgaagc cgatgagcaa acccgccgcg gcaatctgga acgcgcggca
     2281 cagattcagt atggcgagct tccgaaactg gaaaccgaaa tgaagaagct ggacgccgct
     2341 caggatgggg tagccgcgag ctcgcagcgc atgctgaagg aagaagtgga cgaagaagac
     2401 atcgcgaaaa tcgtcagcaa gtggaccggc attccagtgt cgcgcatgct cgaaggcgag
     2461 gtaaagaaac tggtccggat ggaagagcgc ctgaaggagc gcgtggtcgg tcaggacgcg
     2521 gcccttagcg tggtcgcgaa tgcaattcgc cgttcacgcg ccggcctcag cgacccgaag
     2581 cggccgattg gctcgttcat cttcctcggt cccaccggcg tcggcaagac cgaaaccgca
     2641 cgggcactgg ctgaattcct cttcgacgat gagcagtcca tggtgcgcct cgacatgagc
     2701 gagtacatgg agaagcatgc cgtggctcgc atgatcggcg cgcctcctgg ctatgtcggc
     2761 tatgacgaag gcggccagct caccgaagcg gtacgccgtc gtccttactc ggtcatcctg
     2821 ctcgacgaga tcgagaaagc gcatccggat gtcttcaacg tgctgctgca ggtgctcgac
     2881 gacgggcgtc tgaccgacag caagggccgg acagtggact tccgcaacac ggtgctgatc
     2941 atgacctcga acctgggggc aggatttctg accggcgatg cgctgaaaga cgaaacgacc
     3001 ttcgggcacg caacagaaag tgtcatgaag gtgctccgcg agcactttcg tccggagttc
     3061 ctcaatcgcg tcgacgatgt ggtgatcttc cgtccgctgg gcgagcagca gttgcagcac
     3121 atcgtggatc tgcgcctgaa ggatctggaa aaactgctgg cggatcggca catcacgatc
     3181 gagctgaccg atgcggcgcg gcatgccatc ttcgcggccg gctacgaccg ggcttatggc
     3241 gcgcggccgc tgaagcgggc catccagcgg atgattcagg atccgctggc gatgaagatc
     3301 ctcgacggtg aggtgctgca cggagatcac gtgcgcatcg acgcgcataa gggatcgagc
     3361 gcgctgcagt tcgaagtcgt gagccgggcg ggaatcccgc tggagcacgg cgtggccatc
     3421 ggccaggcgt aaagcctcag cgctgagacg agaaaggcct gcgagagcaa tctcgcaggc
     3481 cttttgtcat tttcgtgatc ggcgcgggcc cgggaaggtt caggccgcag ccgccggcgc
     3541 caccggttgc ccgcagtggg agcagcgctt cgccgccagc ggaatatccg ccaggcattc
     3601 cgggcaggga tgcgtcgtgg gaggcgcgac cttcgccgga tggaatttct tcagcagcgc
     3661 gttcatcggc agaacaaaga tgaaatacac gacgcctgca atgagcacga aggaaatgag
     3721 agcgttcaga aattcgccgt acttgatctt gccgccgtgc acatcgaaga tcagagcgga
     3781 gaagttgggc tggtgaacca tcgccgcgat cagcgggttg acgatgtccg tcacaagcgc
     3841 attgacgata gcggtaaagg cggcgccgat gacaacagcg accgccagat ccatcacatt
     3901 gccgcgcagg atgaaatcgc gaaagccttt caacatgagt gtctcccgaa aggatcatgt
     3961 ctcaacagcg tgcgaagaac gtacagcgta tagcgtacag ccgaaacggg acttcccaga
     4021 gcctgctcgc ctgaaatgac cttcgtaccg taggtccctc ggaattctgt tgcattcaaa
     4081 ttgaaaaatt cctaccctga gaacacggat agctctgttt gcaaacgcaa acagagcttt
     4141 tctttttgaa ccagaaccac acccggctca ggccggacga cgcgatcttt gaaaactcgc
     4201 ccagtcgcaa cgcaggccat catactcgtg aatgcatcac tcgcatctgc cagtgatgca
     4261 ttcatgttcg tgaacctgtc attcgtgagc gtcaatgcag cattcacggt cgcctcgaaa
     4321 ctgacgaatc tgcaatcaga tgccttgctc gcgatcgcga atgcagcaaa gacaaccgcg
     4381 aatgcagcat tcaggcccgt ctcccaaatg ccctcccatc agtcagatac cacattgtca
     4441 aacttgtaaa ctccgctaaa cccggcggat tcaatcatct gcaacctggg cccggtgaaa
     4501 atacacaatc gggcgtgttt caatcattgt ccgtctcatt ctcgtcctgc tcgcgaacca
     4561 gggtgcctgt gcgaggtccg tcggcggtat gcacacccca cgccatgcgc ggcgtattgt
     4621 gagccagacc ataacgaccg tccggagcca gcaggatgat gccgccgtgg ccgcccgcgt
     4681 gccgcagcaa ataggccatc gcctccgggg caacctgctc cggcgatctt cctgccagaa
     4741 cgcgatccgt tgcccacttc ccgagaacca gcttcatgat cggctcgccc caccccgtca
     4801 gtgagacagc agccgaggcg ttgtcggcat agcagccgca gccgatcagc gacgagtccc
     4861 ctaccctgcc tggatcctta ttcagggtgc cccccgtgga ggtcccggcc gcaatccgcc
     4921 cctccgcatc gagagcgacc gcgcctaccg tgtcatgcga atcctggttc gagacgttat
     4981 cgccggagaa cgtgcggtcc ggcgcacccg attgctcgcg ggcctgggct tcggccagac
     5041 gctgacgctc ccggtcaaga accagttccg cattgtcgat cagcgtcatt ccctgagcct
     5101 gcgcgaaggc ctcagcgccc gggccgacga aatagacgtg cgggctcttg tccagaacca
     5161 gtcgggcggc ctgaatcgga ttgcggattc gctccacgca agccaccccg ccagcgcgca
     5221 gcgtcgcccc gtccatgatg agggcgtcca gctgaacccg cccatcccgc gtcaggaatg
     5281 agccgcggcc cgcatcgaag gccgggtcgt tctccatgga ggcaacagcc gcttcgaccg
     5341 cgtccagcga cgaaccaccg cgttcgagta tgcgccagcc ttcctggagc gcatcgaaga
     5401 caccttgttc atgagcatcc agttggtccg cggggatatc ccacgcaccg ccatggagaa
     5461 gaaggatcgg ggaacgcgtc ataggaaaga gggattgtat cctgcgctgc gtgacgagtg
     5521 gaattcaagt ggctcccccg cgcgccgata aacgatgcat gctctcgcag aagaggatca
     5581 cggtctacca catggcggcg cgcgagacct cagcgagcga tgccgagatt gtgctgccag
     5641 gccgcggcct gcaatacgag gggaaaatct cgtacctcac gacggagggc tgcctgattg
     5701 ataccaggtg ccggctggaa cccggaacgg tcgtagaggt ctggatgcgg accgagggca
     5761 tgccgctgcg ggtgcttgcg aacctggtgg agcgacggga gcagggggtt gagtttcact
     5821 tccacgaaat gacgaatcgg aagatggacc agatcgagat cctgcgtgga gaactggccg
     5881 aggaggcggc tcggaacgag atcgggcaac aaagccgcgg cgcctgagca cgttgtggtg
     5941 gccgcagtct cacgcgattt cggccgacga atcgtcggtg acgggcacgc ggcccggcag
     6001 gggccgaaag cccgacagca aatggaattt ttctgaaggg gacagggctg cagtggccgc
     6061 cggctccaca gaatagaaca gctttccgtc gatttcgcgc gcccatgcaa tccgcgctct
     6121 ctctgcgccg gccccctcaa tgcgccagcc gtccttcgcc ctgaccagcg tcatggccgc
     6181 gcgctggcgc caccactggt tgatctcgcg cggcaaagcg gtccacacgt tttcggtgtc
     6241 gcggagatgc gccaggtggt ccagcagcgc ctcgaaggtc gcccgcgcct ccggctcgat
     6301 gatgtagtcg ggatggacga tgaagctcat gaggccgtgc cgggcaagga tcatggccgt
     6361 ttgctgtttc cagatgtcag tcgagtactc attgaggatg tggaacagcg agtaatcctg
     6421 tatgtcggtg agcgggatct ccagcagatc gccgatgaag tacggcatca ccgtacagca
     6481 gcctccccgt tgcggatcga ggtgtgcgac gttcggcacc gacatatcgt aggcaaactg
     6541 aaactcgttg aaccagtcct ggttgcgata cagaaccccc gcacggaagc cactggcccc
     6601 aaacttgcgc ccgtattcgt tgatcttcga cacacgctcc agaaattgag cgcgattgtc
     6661 gtagagatgc ccgtcgtgat tgaggtcgtg tactgcgact tcatgaccgc ggtcatggat
     6721 cgaatcaatc agtgtctggg ggacttcgta gcgctcctcg ggaatcacag tgaaggagcc
     6781 cttgataccg cgcgagtcgt cgagatccat caatgtcgcg cagaaatcgc gtcctgcttc
     6841 cgtctcaata tcgtgcgaca tcatggcgca tgccgagtgc ccctcgggcc agaaccatat
     6901 aaatggcacg tgctgaatcc ccgaggcccg tagagccagc acgaggagtt gctcgtggag
     6961 attgtccact gagaaatcga ccggccagcg cgggaaagga cgcctgttcc agttgcgcag
     7021 ccgcaatttc tgcaaatgct tgcgcgcggc caccgacagc aagggtcgaa agaagtagta
     7081 gatcttcgcc gcgagagact gcgtgcccga acgcgcatcg ccgcctgtgt aggcttcgtg
     7141 gcgcagattg gcaatggcct cccggggatc gaacgggaga ccgatggcgc cgtcggtgat
     7201 cgccacgtcc gcactcgcgt catgcagggg ttcctccggc gaagcagccg ggacctgagg
     7261 cgccagccgc ccgtacagcg tggtctccgg accgaaggca aagtaccccg ccggcgcagc
     7321 cgctgctggc gccgcaacac gcactccctc cagaaaatcg ccagggcagc gaaagtacac
     7381 ggagagaggg cccaaaaact cgtccgtttc gcacataggt gtcacgacag catattgacc
     7441 acttttggcg gtccgcaact gatctttagg ttaaaaaaaa tggctcgaca gtacccgttt
     7501 ttgtggccct gttgaaaact ttcgcgacgt cactttggtc gcagcaaaca ctgtccggga
     7561 acccggcggg aaacgattgg gaagcccttt ggcgcggaaa aagcgggaca ttccggcgac
     7621 cgcgtactat tctggagcca aagccccagg ggcctcccca gcccattcct gaacgcgttg
     7681 atccggcctg ttcgatctgg ccgtacccgg aatcgcattt catgtcagaa agctcgaagc
     7741 gaccgccgag tgaaatgccg acgcagtttg gctccgccac cgcggtgagc agctcggcgg
     7801 acgacctgcg cgcgctggaa acgatcgtgc ctgcgtcgaa ggaatcccga gccgacgaaa
     7861 ccctcgtgcc cccatccaaa agcgacgcgg ccaccatggt gcccccctcg cggtcggatg
     7921 cagtgaccgt cgtgcctgga agcagcgatc ccgcgggcgg atctggaggc ggttcgcgca
     7981 ccggtggttc cgctggatgg accagcgaaa cggcagaact ggaacctggc acggtcctgg
     8041 ccggccggta cgagattcgc gctgtgctcg gcaccggcgg catgggatct gtctaccgcg
     8101 cgcacgaccg ggagctggat cgactggttg cgctcaaggt gattcggccg gaactggcgc
     8161 gaaaccccgc gatcgtggat cggttcaagc aggaattacg gctctcccat aaggtcacgc
     8221 accgcaatgt ggtgcggatg tacgacctct ccgaagacgc agggatgcgt tttgtgacca
     8281 tggagctggt ctccggtcgt gacctgcgca cagttcttga agagcgtggg aaactaccaa
     8341 ccgatgaggc agccactgtt ctggagcaga tttgctttgc gctcgacgcg gcgcatagcc
     8401 aggggattct gcaccgtgat ctgaagccgc agaacgtgat ggtggaagaa aacgggcggg
     8461 tcgtcgtgat ggacttcggc ctggcccgca ccatcgaagg cgatggcatg acccagtcgg
     8521 gcgccatggt cggcacgatg gagtacatgt ccccggagca ggcgctgggc aaagaactcg
     8581 atcagcggtc cgacatcttc gcgctcgggc tgatcgggta cgagatgctg accggcgcca
     8641 tgccgttcaa ggcagagagc gccattgcca gcctgctgaa gcggactcgc gagtcggcgg
     8701 cgccactgat gaaggtcgat tcttcgattc ccgctgtgtt gagcggcatc atcgaaaagt
     8761 gtctgaccgt cgatgtcgaa cagcgctaca agagcgtcgg cgaggtgctg cacgaccttg
     8821 aagtctggcg aggcaaagcc gcagcggcga gcctgcactt taaagccgac gtaccctcga
     8881 caggcctgag cgggcgatgg ctgttcatca cgggcggcgg ggctttgctc attgccctgg
     8941 tcgccggtat ttccctgggt gtgcggcatt tctctggttc gcagaccacg gcgacgccgg
     9001 cgagcgcccc atcgatttcg ctggcgatca tgccgttcta taacgcctcc ggggactcgt
     9061 cgatggactg gctcggggcc agccttgccg aaatgctgtc gaccgacatt ggcggatcct
     9121 ccgccgtgcg catggtttcg cccgaccgcc tccagcaagt actcggagac ttgcatctca
     9181 cagcgaactc ccaggccgac gcatcgactt tgaagcgagt cgctgagttc accaatgccc
     9241 agaccatcgt ctacggccag ttcttgaagg ccggcaacac gattcgtatt agttcgacgg
     9301 tggtggatct cgcccacgac ggagttcgat cgaccgtaac aaccgacgtt cccgaccaga
     9361 accaattgct cgccagcgtg gataagctcg caggccaatt gcgcgagaag ctgacagcag
     9421 acccgaacgt gctcaacgat ctgaaggcgc atgccgtgcg cccctcgacc acatccgttg
     9481 aggccctgca cgactacgag aacggactgg cgctggagcg cgcaggcgat aacctccggg
     9541 cgattgaagc tttccatgcc gccaccgagg cggataccaa cttcgccacc ggcttcgctc
     9601 atctggcgca ggcgtattcc agccaggggc aggacgatct cgcgcagtcc gcctcgcgga
     9661 ccgcgatgga actggctgca aaccttcccg ccggggagcg atatcgcatc gaggccagca
     9721 acgccgaaat caacaacaac accgagaagg cgattcaggc ctatcagcag ctcgcggcgg
     9781 ccaatccgtc ggacaccgac gtgcagttcg ccctggcccg gctctacgag cagaacggtg
     9841 actatgcgga agcccggcaa catctcgccg cagtgctggc gggcgatccc aaaaacgtgg
     9901 cggcgcttct cgcgagcggc cgggtcgcga ttcgcggtgg cgatgcgaag ggcggccttg
     9961 attttctcag caaggcgctg cccatcgcgg tcgagctgaa caaccagcag caaaaggccg
    10021 caatcctgca ggcgacaggt attgcctaca gcctgatggg gcgtcctgat gatgcactga
    10081 ataacttcca gcagtcgctg gcgatcaagc agcagatcgg cgacaaacgc ggcgcggcag
    10141 cgagcctgga gcaggtgggt tcactgcagg ctgctgcggg caaagcggat gcggccctga
    10201 aaagctatca gcaggctctc tcgctgcgga aagagctggg agatcagtcg ggtattggca
    10261 acacgctgat cgacctcggg tctttctacg gtaatcacgg caagtcggac caggcgctgc
    10321 agtactacac cgatgctctt caggtggagc gccagctggg cgatgtcgcc aaccaggcgc
    10381 tttgcctgaa caatatcggc tcgatcaagg tcgatcaggg ccactatcag gatgcgctga
    10441 cctatctgga gcagagctac gatctgcgcc agaagctgaa tgtccccgcc gacatcgggg
    10501 agtcgctcca cgatcttgct gaagtcaata cgaaactggg tcagtacgat accgcgcttt
    10561 cgtattattt gaaggcgatc gcagcttacc gcagcgcaaa cgaccagcat ggagtggcca
    10621 tggcctccga tgcgatggct cggctcttcg ccgcacaggg ccgctatggc gcggcgctgc
    10681 actcgatgca ggatgccgtc agcatccttc ggcagtcgaa ggagatgacg tggctcaccg
    10741 tggaggccat cggcggctgg ggcgatctgc tggcgcaggt ggggcgcgct ccggaaggca
    10801 aagcgagcct cgaggaagct ctgaatgacg cccaccagat caaagacgat gcgtatgtcg
    10861 cgatcgcaac caatctgatg ggtgactact actactacca gggcgactat ccgaacgcgc
    10921 gcgcggagta tgcgcaggcg caaacgattg ccgcgaagac aacggacaga gagacacagt
    10981 tgcgatcgaa gatgaacgtg gcgaagacgg acctcgctct tggtcgcgca gcagcggctg
    11041 ttcctgtgct gaagaagctg acgcaggacg ccgacgcact cggcctgaag gccgaagccg
    11101 tggagtgttc catctacctg accgaggccc tgatggcgac gaagaacgat gcggcagccg
    11161 gacaaaccct cactttggcg caggcgcgcg cggagaatct ggggcttcgg attcttcagg
    11221 tcaaggccga ttcgctcgca gcagcgctgg ctgcgaagag tggcaggacg gcggaagcgc
    11281 agcggaaccg gcaacaggct tcacggattc tggatgccat tgtcaaagaa gacggcgccg
    11341 ccagggccgc gcaacgcgcg gatctaaagc agcttgcagc cagcactcaa tagccccgtc
    11401 agccgtcatt ggagccggcc ggccaatctg caagccggag agaagcaagc ccgaacgtgc
    11461 ggacaagacg ccgccagacg gcgggaccga ccagtacatg cgggcccgtt gctggcaaac
    11521 gcgaatcccg gtggcagaac caccgggatt cgcattcgtg ggtgcgggcc ggttttgcga
    11581 gccctagttc ccggtgcccg agagcgatat cgcttgaggg ctgttgaagg cgttgtcggt
    11641 tacgatcagc gtcgcggagc gcgaaccctt ggctgccggc ttgaacgtca ccccgattga
    11701 acaggtcccc ttcggcgcca gtggggccgt gcagccgttt gtcgggtgaa agtcagccgc
    11761 gttcccgccg ctcacgctga cgttgctgat ggtcagcgac gtagcgccgg tgttcgttat
    11821 ggtcaccgat tgcggtgaac tggtggttcc tgtctttacc ttcccgaagt tcaggcttcc
    11881 ggtgctgaac ttcgcaatcg gattgatcac cgtggctgtc aacggcaccg tgtggggact
    11941 tccggccgca ctatcgttga tcgtcagcgt cgcggtctgc ggcgtgtagt acggtccggc
    12001 gataaacgtt acggtcatca cgcagctctt gccggccgcg agcgatttgg ggcaaaggtt
    12061 cagggtgacg aattctttgg aattcccccc tttaacgatc gcgatcagcg ggtcgtgaat
    12121 ggtcacagca ccggtaccgg tattggtgac ggtcacgatc ttcgtgacga tcgaccccaa
    12181 ataaagcgtg ccgaagtcga tggacgaggg attaatgttc agcatctgcc ccactggaat
    12241 ggtgaggatg aggggcgttg cattgctgag caggtgggcc gtgtcgccca aatactgcgc
    12301 cgtgatcgtg ctcggtccgg tcgtggtcgg ggtgtagctg caggttgctg tcgtgacccc
    12361 ggcggtggtg ctcaccggac tgctgcagag cagagtcgct cccgcgtaga acaaaacgtt
    12421 cccggtgggc ggggcaatct gcgtcgctga accggcaacg gtcgccgtca gcagatcggg
    12481 ttgacccagc gtcggtgtca tggaggcggc gctgagctga gtctgtgtcg tggcattgtt
    12541 cgtaacgctg gtgaagacaa agtcggagaa gttgagcgtc gtgcccttgg acgtgccgtc
    12601 gatgttggct ggcgtgtagg acacgaagat gttctgcagg ttgttcgaag tcagatatcc
    12661 agcggtggtg gccgacccgg atgccccgac cgcggtttgg gcgacgaagg cattcggcgc
    12721 agacactgag gtgaccgtat agctgccgtt gaaaccggcc ggagtggcgc caatcacgga
    12781 aactgtttgc ccgatcgtga cggagcattc gcccgtgcag gtgaagttcg ccgtatcgcc
    12841 gctgacattg agggcgctga tctggctaaa gaagggatcg ccctgcagat agcccggcga
    12901 gacaggcgtc acgctgctgt taaagacggt cttgacgttg atgcacgatg cagcgttggc
    12961 tccgatacag ttcggcacgc tggtgacggc aggacacgct acgacctgat ccggatggcc
    13021 cgtgacgaag cacgagacgg aataaatgat gcagttcccg cctgtgtcgt catacacctg
    13081 gcaggcggaa ccctggaagg gggtgcccgc aatcagttgc gagaagagcg tcggcgagac
    13141 cgtattggcc gtcaccgtca tgctggtgcc ggtcgttgca ggaccatcgc ctggatattg
    13201 cactccgtag gtgtgcgtgc cgtacgggaa ctgcgtatag gtgggactgc cggcgggctg
    13261 caggggctgc tgctgcgtga caggcgtatg aggagtaaag gtccacccca ccagttcgtt
    13321 ttgctcgaac gcggcgcccg ttgcggaagt gaaaccaatg taggcgctgt tcgggcccga
    13381 tccggtgtcg agcaggttca gcgcctgcgt gatgtcgtag gggccggaga aaagcgggac
    13441 agagcctgcc accggtgtga cggttcctgg gtcaaaggcg ggatcgagat aaacgtagag
    13501 gtaattcgcc ggggaatcac tcggcccgtt gtaaaccacg accacctggt ggacatttcc
    13561 gtcggaaagg acgacgggca ccggtgcggc attcgccacc agggaagcgt tcggattaga
    13621 gaccagcgtt gggatctggg tctcgtctgg accgggtagg tagacctcgc aggcagggcc
    13681 gctgtccgaa gcgatgtgag ctgcggagtt aacctgccct gctccgcagt cctgcagcgc
    13741 aatatgattg tcgtcgttga aattgccgtg cgccgcctca tcggggtcaa aggagttgaa
    13801 gtacgtatcg aattccaacg cgacactgtt gtcgatgccg ctgtatccca ggcacccccc
    13861 gccaccgcca aggacagtcg ggccggatcc agtttcctga cagccgctgc ttggatcgac
    13921 ggctcccccg ccatgcgcat tttgaatcac gaaggcgatt ccgtccgcgg tggcgtagct
    13981 gtagccggtg ttatccgggg tgagcttgaa ttcaaaccat gcattgaatc cgccggaaac
    14041 gacctgcggt acggagaacc acaggctgcc agcctggctc gcagcatcgg gagtcatctg
    14101 cgtggcccat gtgccgctgc ccaccgggtc ctggataaac aatggattgc tcatggtgcc
    14161 ggtaccgtca ttgaggcaaa agagcggatt cgcggaagga ttcgcggcgg agcaggtata
    14221 ggagtactgg aggggcgatg ccgtggccgg agtgtcgaag ttgtagtagg tgatctgctg
    14281 cgcggaagcg cggactggca ggagggcaaa cagagtagcg gcggcgcaca cgacaacgtg
    14341 cagggctttt gcggatgcga gcttcatgcc atgtccttcg gagctactgg gaacgctcct
    14401 gacgcagatt tgatccgggg actactccaa ctctgaacgc gagagggaag ccaactgggg
    14461 aacggcagct ctgctgcgac gtttccgatg ggtttagcgc gtggtcagaa tgataatcac
    14521 cgcgccgggg aaagtgtgta aaaattcgta accgccgctg gtcacattat tcggagagtt
    14581 aacgggtcga ctgcgtgtta cgcaggcgca tgcacgcgaa attgggcgaa ttcgcgttaa
    14641 ccatgccgcg attttctccg ataaccagag cgtgaccagc agcgcgctcc aaaatggcag
    14701 attctctgta gcgctggtgg gactgggttt gcttctggct ctgccgagcc ttgcgcgcgc
    14761 gcaagccgcc gatcaggatc tgatggcgat gcctctggag gacctggcgc gggtgcaggt
    14821 ctatagcgcg tcccggcatt cggaagacgt caggaaggct ccatcgtcgg tcagcatcat
    14881 cacgtcggaa gacatccgcc gctatgggtg gcgcacgctg ggcgaggcgc tgcggagtct
    14941 gcgcggtttc tatacggcgt acgaccggca atacacgtat ttgggcattc gcggatttct
    15001 gcgccctgga gacttcaatt cacggatcct tctgctggtt aacggacacc gttacaacga
    15061 ggacgtgtac gacagcgcgt cgttcgactc tgagtatccc atcgatctga atctggtgga
    15121 tcacattgag gttgtgcggg gtcccggatc gtcgctgtac ggaaccaacg ccgtgtttgg
    15181 cgtcatcaac gtcatcacgc gccagccagg ttcggagacg gtcgcggaag tggccggcac
    15241 cacggggaca ttcctgcagc ggacagcacg ggcgacgttg cagggccgcg tgggtggggt
    15301 ttccggattg ctgtcggcga cgctggatcg caaccccgga gcgccagttc tgttctttcc
    15361 cgaattcgct gtgccggaga cgaacaacgg ctatgcggag tccagcgatg gcggaagcat
    15421 gcagcaggga ttcgccgatc tcgcctgggg cgactttcgg gtgcagggaa tgttcggcaa
    15481 tcgtgtgaag gtaattccga cggcctccta tggcatggtg ttcaacgacc ctgcggattc
    15541 cgatcaggac acgcgggcat atgtcgatgt gagcgaccgt cggggggtcg gcaagggccg
    15601 gacgctcaat ctgcgggctt actacgatgt ttacaactat gtgggccggg gagactatgc
    15661 ggagccagga aacgaggctc ctgtgcggat ctacgggaag gcccgcgcgg actgggttgg
    15721 aactgaagcg accctgacct ggcaggcggg cacgcagacg gtcatagccg gggccagtta
    15781 cgagcaggac atccacgtga cgcagcgctc ggttgcggcg gaactgccgg acatgctcca
    15841 catcgaagaa tcgccctggc tggtcgcggc ctacggagag atggaactgc acatgctgcc
    15901 gaaaacgatc ctgcacgcgg gaggcaggct cgaccattac agtaccttcg gcgccgcatt
    15961 cagtccacgg ctggcggcga tctggacgcc gggagaccgg acctcagtca agtacatcct
    16021 tgcgggagcg ttccgggcgc cgagttcgta tgaagagttt tacgccgacg ggatgacgat
    16081 cgagccggca tcacggccgc tccagccgga gcgcatccgg tcgaacgagt tagtgttcga
    16141 gcgctcactt gcgaaaggag tgcgcttgac cgtagacggc tacgatgacc ggctggagaa
    16201 gttgatcgat cagagtccgg atttggccac gggtctcacc ttctttacaa acgttggacg
    16261 cataagttcc cgcggtctgg aggtggagct ggaggcagag cggccgtcgg gatggagcgg
    16321 ccatgccacc tatacggcaa cggcgacgac caacgatctt ttgggaagac cactggagaa
    16381 ctcacccatc acgcaggtga aagtggcgag cagccttgct ctgggccgat gggttaccgg
    16441 cagcgcagaa gtcatgtacg tcggccccat gagggactat cgccagggcc gtctcgcctc
    16501 gtacgttttg ccggatgtca cctttgctag ccggccgctg cgaaacggct ggcagttttc
    16561 ctccagcctc tacaacgtgt tcgatcgtcg ctggtacagt ccggccggcc ccaacgatcc
    16621 gcagagtcag attcagatgg acggacgctc gtggcgcatt caggttggct atcgttggcc
    16681 gatgcgaggc ggcaggaggg agcagtgaaa cacgtggccg tctggcggtg cctggcatgg
    16741 gggctggtct tctggctgcc aggagcgggg ccagtctcat cgtcgacaat gcgagcccag
    16801 tcgcgcgacg agcgggcggt acgggcggcg tatgtctaca acctgatcaa gtacgtcgaa
    16861 tggcccgcac cgggaaagga cctgaccatc gctcttgtgg gtgatgccgg caccggcgag
    16921 gtgattagcc agttgctgaa cgggcgaacc agagagggtc agacgctccg tgtggtgcag
    16981 cccgtgtcgg ttgaggcact gcaggggtgc agtgtcgtct atgtttccgc tgcaccggaa
    17041 agcgatatcc ggaagatcct cgagagagtg aagggccggc cgatccttac cgttggagag
    17101 agtgacgggt tcgcgcgcgc gggtggaatg gtcgcgctgg tcaacacggg agatcacatt
    17161 cagattgaag tgaatctgga agctgttcag gccggcggga ttcgcatcag ctcgcgcgta
    17221 ctgggcctgg cgacgatcgt acattcggcc gcgaagggcg gggacggacg atgagccctc
    17281 ctgttcgcgc cagctttgcg catcgcatga cgatcctcgc tctccttgcc agcagcgtgt
    17341 cgtcggcgac cctgatggcc gcatttcttg gattcgactc ggtcagtgcg cgccgccagc
    17401 tggagagccg gttgtcgatt ctgacgaaca tcgtagggca gaattccgca gctgcactgc
    17461 tgttcgacga tcgcgccgcc gcgatggaag tactcggcgc gctggatgct gagcggtcag
    17521 tggtctcggc ctgtttgtac ggtgtgtcgg gcagattgtt tgccgaatat cagcgcgaga
    17581 aagcaccgca aagctgtcct gagcagttcg tgccggccgg cggaaccgct cgagggttcg
    17641 tgaccatgag ccggccggta gaacgcggtg gcgatgcggc tggcacgctg gtgctcaggg
    17701 ccgatgtgaa ggagattgtc gaacgctggc gtcagatgct ggagattacc gcgttgcttc
    17761 tgctgggagc actggctgcg ggtgggatcg ccggctcgtt gttgcaacga cggatttcac
    17821 agccagtcca ggcattggcg gctgccatgc acgaagtgac tgaacggcac aattttggtg
    17881 cacgagtggc gaccgagggc aatgacgaga tcgcgcggct cggcggtggc ttcaacacca
    17941 tgctggcaga gctggaaaag cgcgccgcgg agaaaactgc ctttgaagcg cagttgcagc
    18001 gccaggcgat gaacgacgat ctgaccggcc tcccgaatcg gcggctgctg gccgatcggc
    18061 tgacccacgc tctggatttg gcgcgacgaa gaggctatga ggtcgccctg ctctacatcg
    18121 atctggacgg gttcaagctg gtgaacgaca gcctcggtca cgcggtgggg gatcagctgc
    18181 tgcgccaggt cgctgagcgt ctgcggtcgc gagtgcgctc atccgacact ctcgcacgac
    18241 tggggggcga cgaatttgca gttgtggtga gcggcgagaa ggtacgcgaa cagggaagcg
    18301 ttgccgcgat ggcgctgctg gcggtcctgg cgccctcctt cagcgtggaa gagcacgaga
    18361 tcacgatcag cgcaagcatc ggaatgagcc tcttccccga aaatggttcg accccgatcg
    18421 aactgctgca gcaggccgac ggcgcgatgt acgccgcaaa acgaatgggc aagaaccgcc
    18481 tcatggcatt ttctgacgaa ctcggtgcat ccatacgcga acgtctgaaa ctggaaactg
    18541 aacttcgcca tgcgatcgca cagggcgaga tacgcgcgta ctatcagccg gagttcgacg
    18601 tcaacaccgg agaactgatg cggttcgaag cactggcgcg ctggattcac ccgacgctcg
    18661 gcttcatctc gcccggccag ttcattccca ttgcagaaga gactggcctg atcacagccc
    18721 tcgggcaaca catgatggaa atcgcttgcc gcgaggccat ggaatggcag gaaattgccg
    18781 gtcgcgctgt gcaggtggcg gtgaacgtct ccagccttca gctcatgagg gagacgtttg
    18841 tcgaggaagt ggcggagatt ctgcggaaaa ccggcctcga tgcgcatcgc ctgcaaatcg
    18901 agctgaccga gtcggtaact gtgaatggtg cggcacaggt tggcgcagcc atgggcgaac
    18961 tggcggaact cggcgtaagt ctggccatcg acgattttgg aactggctac tcctgcctga
    19021 gctatttgcc caaactgccc ttcgacgcgc tcaaaattga tcgctccttt gttagcgaac
    19081 ttgggattcg ccgggaggtg gatgccatgg tccattcgct cgtgaccctg gcccacaact
    19141 tcggaatgag ggtcatcgcc gaaggcgtgg aaacagaaga gcagatggct ctgatcacgg
    19201 aactcgggtg caacgaagtc cagggatatc tgctcggtaa acccacgagt gatccgcggg
    19261 cctgcctggc cagtgccgtc ggcaaccgcc gctgcggtga caaaatcacc gcagacacga
    19321 agatactgac atcagaaaca atttgaggac atgctcatcc gcctaattcc cccgagcgga
    19381 ggcacgaagc cgagctggaa gaaagggatg aatcagaagt tgataggctc ctccgcccac
    19441 cgctccgccg agcagcggcc ccaggatcgg aacccaccag tagctttgcg gtgagggcaa
    19501 cgccgagcct ccccagcctg cgaagaagca aaaaagccgt ggaccgaagt cgcgtgcagg
    19561 gttgatggcc catgcctcca gatagcccat ggaagcacca atcagggcca caaggaagcc
    19621 aatcatgagc gcgccaaagt tcgcacccgg cgccatctcg ttgtattgct cggtaatcgc
    19681 gaatatgcca aaaatcagga atgccgtgag aacgatttcg tcgccaaacg cgtgga
//
LOCUS       DASZYN010000121         4120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_133638, whole genome shotgun sequence.
ACCESSION   DASZYN010000121 DASZYN010000000
VERSION     DASZYN010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133638"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VHE33_03125"
     CDS             complement(<1..609)
                     /locus_tag="VHE33_03125"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVT96469.1"
                     /translation="MFPENRSRLAGATVVLAVLAIAWIVHVRHPQFLWAAAGVGLAWV
                     AMELTARKARREHDALASLLAERTVERDQQQQARAELQRELDRQTTHDKLTGVWNRAA
                     IPEHLEREMVRAQRDVKVLAVVIADLDHFRKINDTRGNLCGDRVLRTAAERLAFCLRE
                     YDSIGRYGGEEFLILIPGYDPAEGRSRLDELVRSVHDHRFLND"
     gene            932..1765
                     /gene="ppk2"
                     /locus_tag="VHE33_03130"
     CDS             932..1765
                     /gene="ppk2"
                     /locus_tag="VHE33_03130"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HVT96470.1"
                     /translation="MAKKRKGTSAKGHVNGVAHRAAGEYSYKKELRRLQIELVKLQDW
                     IRHEGLRVVVLFEGRDAAGKGGAIKRITEILNPRICRIVALGTPTEREKTQWYFQRYV
                     AHLPAAGEMVLFDRSWYNRAGVERVMGFCNEAQYREFMHSCPEFERMLIRSGITLIKY
                     WFSVSDDEQERRFRERIENPTKRWKLSPMDLESRRHWADYSRAKDEMFAMTDTPQAPW
                     FVVNADSKKQARLNVIHHLLTRIDYKELIPQKLKLPQLDRRKYTRPPMHEQNFVPAVY
                     R"
     gene            1855..2601
                     /locus_tag="VHE33_03135"
     CDS             1855..2601
                     /locus_tag="VHE33_03135"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HVT96471.1"
                     /translation="MPRLSSGVLGFPDFRGFTRALILWNLASYFGLLILGVFSAPLAN
                     QIAALLALDPSLVLHHYWIWQLLTYCIIHPGGAILATAFELLSLWFLGSLLESGHGSR
                     WLAELFFVSVFGAGITAVGLYFVFGAGAVLYGCFGGIFGLLIAIGVLYADTPFMMFPL
                     PITIKAKYLVAVYMLISLAMLFSSQRAYAFAQLGGALFGYLYIKFSPRRGFAFAGSES
                     MYNLRNRYYRWKRRRAAKKFEVYMRKHRDN"
     gene            complement(2896..3675)
                     /locus_tag="VHE33_03140"
     CDS             complement(2896..3675)
                     /locus_tag="VHE33_03140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT96472.1"
                     /translation="MARPVAVVTGASSGIGEVFARKLAAAHDLLLIARRRDRLERLAS
                     ELMEQSDARVEVLEADLASEPGLALAAERIGSDPNLELLVNNAGFGLSGYLWQADRSR
                     QEEMHRLHVMAVVRLCHAALKTMVERNAGGIINVASVAGFVHAPGSASYGATKAWMTH
                     FTEGLYLDLKAAKSAVQVQALCPGFTYSEFHDVLGRDRTKLAPRSWWLDANFVVEESL
                     RGLRRGKLLVVPDWKYRTLVRVVTRLPVGLRVWVEGQRNRT"
     gene            3778..>4120
                     /locus_tag="VHE33_03145"
     CDS             3778..>4120
                     /locus_tag="VHE33_03145"
                     /inference="COORDINATES: protein motif:HMM:NF015228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96473.1"
                     /translation="MTDQAATNPPPVSPEWAKTELARHPQRPYPMDFIERICTDFSEI
                     HGDRSFGDDPAMACGMARFDGQEIMVVANLKGRSTKDKVSRRFGMPNPEGYRKALRCM
                     QIAEKFARPVLS"
BASE COUNT          766 a         1492 c         1058 g          804 t
ORIGIN      
        1 atcgttcaga aaacggtgat cgtgaacgct ccgcaccagc tcgtccagac gactccgccc
       61 ttccgccgga tcgtatcccg gaatcaggat caggaactcc tcgcccccgt accgtccgat
      121 gctgtcgtac tcgcgcaggc aaaaagccag gcgctccgcc gccgtgcgca gcacccgatc
      181 gccgcagaga ttgccccgcg tgtcgttgat cttccggaag tggtccagat ccgcaatcac
      241 caccgccagc actttcacat ctcgctgtgc gcgcaccatc tcccgctcca ggtgctccgg
      301 gatggccgcc cgattccaca ccccggtcag cttgtcgtgc gtcgtttgcc gatcgagctc
      361 ccgttgcagc tccgcgcgag cctgctgctg ctggtcccgc tccacagttc gctccgccag
      421 aaggctcgcg agggcgtcgt gctctcgcct tgccttccgg gctgtcagtt ccatggcaac
      481 ccaggccagc ccaactccag ccgctgccca aagaaactgc ggatggcgaa cgtgcacaat
      541 ccaggcaatg gccagcaccg ccagcactac cgtcgctcca gccagcctcg atctgttttc
      601 cgggaacatt ctggtccacc gatcctaatc gcgcccagag cgattgaata tcccgcgaat
      661 gaaccatgcc ctgggacaga acacctcagt aacagtccgc gagtccctgg tgtggtgttt
      721 ctgaaggtcc tttcccttgt catcccgacc ggaccctgag cgtagcgaag gggaagcgga
      781 gggacctgct tctcttccgc ataaagaaat ttcggaaaca ccacaccagg atcccgcaac
      841 tccatggtcc ctgcaggaaa actgccgata aggctcacga cagctgcgtg gggaccggcc
      901 tggttgggcc gcgcagtctc agggtgatgt tgtggcaaaa aagcgtaaag gaacctctgc
      961 aaagggacat gtcaacggcg tcgcccaccg tgccgccgga gaatacagct ataaaaagga
     1021 actgcgacgg ttacaaatag aactggtcaa gctgcaggat tggatccgcc atgaagggct
     1081 tcgcgtcgtt gtcctctttg aaggtcgcga tgccgccggt aaaggcggcg ccatcaagcg
     1141 cattaccgaa atcctgaatc cgcgcatctg ccgcatcgtg gccctcggca cccccaccga
     1201 gcgcgagaaa actcagtggt acttccagcg ttatgtcgcg cacctgcccg ccgccggtga
     1261 aatggttctc ttcgaccgca gttggtataa ccgcgccggc gtcgagcgcg tgatgggctt
     1321 ctgcaatgaa gcgcagtatc gggagttcat gcacagctgc ccggaattcg agcgcatgct
     1381 catccgctcc ggcatcaccc tcatcaagta ctggttctcg gtgagcgacg acgaacagga
     1441 acgccgtttc cgcgaacgga tcgagaaccc caccaagcgc tggaagctca gccccatgga
     1501 tctggaatcc cgccgccact gggccgacta ttcccgcgcc aaagacgaaa tgttcgccat
     1561 gaccgatacc ccccaggcgc cctggttcgt tgtcaacgcc gacagcaaga agcaggctcg
     1621 tctcaacgtc atccatcatc tgctcactcg catcgactac aaggagctca ttccccagaa
     1681 gctcaagctg ccgcagctcg atcgccgcaa atacacccgg ccgcccatgc acgagcagaa
     1741 ctttgttccc gccgtctacc gctgagcgcg cactggcaac ccttcgaaat atccggcccg
     1801 tcgccgtctc ggtgcagatt cgtcccggct cgcgcggatg agacaatatc catcgtgcct
     1861 cgtctttcca gcggcgttct cggcttcccc gacttccgcg gcttcacccg cgcgctgatc
     1921 ctctggaacc tcgcttccta cttcggcctg ctcatcctcg gcgtcttctc ggctccgctc
     1981 gcgaaccaga tcgcggccct gctggccctt gacccatccc tggtcctcca ccactactgg
     2041 atctggcagc ttctcacgta ctgcatcatt caccccggcg gcgccattct tgccactgcc
     2101 tttgagctgc tctcgctctg gttcctcggc agtctgctcg agtccggcca cggctcccgc
     2161 tggctcgccg agctcttctt cgtctccgtg ttcggtgcgg gcatcacagc cgtcggcctc
     2221 tatttcgtgt tcggcgctgg cgcggtcctt tatggatgct tcggcggcat cttcggcctg
     2281 ctcatcgcca tcggcgtcct ctatgccgac acgcccttca tgatgtttcc cctgcccatc
     2341 accatcaagg ccaaatatct cgtcgctgtc tacatgctga tctcgctcgc gatgctcttc
     2401 tcctcgcagc gcgcctacgc cttcgcccag ctgggcggcg ccctcttcgg ctacctctac
     2461 attaagtttt ctccgcgccg aggcttcgcc ttcgccggca gcgaatccat gtacaacctg
     2521 cgcaaccgct actaccgctg gaaacgccgc cgagccgcaa agaaattcga agtctacatg
     2581 cgcaaacacc gcgacaacta gtgtggcgtt tctgaagtct cttcaccttg tcatcccgac
     2641 cggaccctga gcgtcagcga agggggagcg gagggacctg cttctccttc ttcaccctgt
     2701 atcccgaccg gacccttcgc gcagcgaagg gggagcggag ggacctactt ctccttcttc
     2761 accctgtcat cccgaccgga cccttcgcgc accgaaggtg agcggaggga cctgcttctc
     2821 cttcttcacc ttgtcatccc gaccggaacc ttcgcgcagc gaagggggag cggagggacc
     2881 tgcttctctt cagatctacg tccggttccg ctgtccctca acccatacgc gcaacccaac
     2941 cggcaggcga gtgacgacgc gaaccagcgt cctgtacttc cagtccggca ccaccagcaa
     3001 tttcccgcgc cgcaaaccgc gtagcgactc ctccacaaca aagttcgcgt ccagccacca
     3061 cgaccgcggc gccagcttcg tccgatcccg ccccagcaca tcgtgaaact ccgagtacgt
     3121 gaaacccgga cacagggcct ggacctggac cgccgacttc gccgccttca ggtccagata
     3181 aagaccttcg gtaaagtgcg tcatccacgc cttcgtcgcg ccgtaactcg cgctgccggg
     3241 cgcgtgcacg aaacccgcca ccgaggcgac attgatgatg ccgcctgcgt ttcgctccac
     3301 catcgtcttc aacgctgcgt gacacagccg caccaccgcc atcacatgca ggcggtgcat
     3361 ctcctcctgg cggctccgat cggcctgcca gaggtatccc gacaggccga aacccgcgtt
     3421 gttcaccagc aactccaggt tcgggtcgct gccgatcctt tccgctgcca gtgccagccc
     3481 cggctcgctg gcaaggtctg cttccaacac ctccacgcgc gcgtccgatt gctccattag
     3541 ctcggacgca agtctctcca gtctgtcccg acgacgggca atcagcaaaa gatcgtgtgc
     3601 cgccgccagc tttcgcgcaa atacctcgcc aatcccgctc gacgctcccg tcaccacggc
     3661 taccggtctt gccatgccac gctcctgcgc cgatcttagc gaattcgccg agacagcccg
     3721 cgcatttgct ccgccctgct cccatccata caatcaatct cggactgcct ccctcttatg
     3781 accgatcagg ctgcgaccaa tccgccaccg gtttcgccgg aatgggcgaa aaccgaactg
     3841 gcacgtcacc cccaacggcc ttaccccatg gacttcatcg agcggatctg caccgatttc
     3901 tccgagattc acggcgaccg ctcctttggc gacgatcccg ccatggcctg cggcatggcc
     3961 cgcttcgacg gccaggaaat catggtggtt gccaacctga agggccgctc caccaaagac
     4021 aaagtgagcc gccgcttcgg catgcccaac cccgagggct atcgcaaagc tctgcgctgc
     4081 atgcagattg ccgaaaaatt cgcgcgtccc gtcctctcac
//
LOCUS       DASZYN010000122         3081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_712377, whole genome shotgun sequence.
ACCESSION   DASZYN010000122 DASZYN010000000
VERSION     DASZYN010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712377"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VHE33_03150"
     CDS             complement(<1..692)
                     /locus_tag="VHE33_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96474.1"
                     /translation="MATRQSTTETAEHSRAVRLPQTLGELRSSADYSEQRLRTRTVKD
                     EMRENLICRLKSKQAIFPGIIGYEDTVVPQIVNAVLSRQNFILLGLRGQAKSRILRAL
                     TALLDEHTPYVAGCEIRDNPYAPLCRRCRDLIVEQGDATPLGWMTRDERYVEKLATPD
                     VTVADLIGDLDPIKAAKGGHDLASEMTMHYGLLPRANRGIFAVNELPDLAGKIQVALF
                     NIMQEGDVQIKG"
     gene            complement(778..1383)
                     /locus_tag="VHE33_03155"
     CDS             complement(778..1383)
                     /locus_tag="VHE33_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HVT96475.1"
                     /translation="MRIPLFPLNTVLFPGAALPLHIFEGRYREMVGDCLADDRPFGVV
                     RADGEQMSIVGCTAKIVRVVQRYDDGRMDILCEGAQRFEIEALDESRTFLQADVEFFE
                     DEGAGSTRDEREDCAALHFATLHMAGIQPPALHLDLNTPVAFQLADALPSDLGFKQQL
                     LASRSDADRTRKLRDFYNDMLPRLSSKPPAGQAGHSSFPIH"
     gene            complement(1435..1752)
                     /locus_tag="VHE33_03160"
     CDS             complement(1435..1752)
                     /locus_tag="VHE33_03160"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT96476.1"
                     /translation="MDKVAGLKEILEQDPSNAFARYGLAMEYSGRGQTESALAEFTQL
                     LKDHPDYTAAYFMAAQTLAKAGRDAEAKQKLVDGLACAKRTGNQHAAREMQAMLDEME
                     IPE"
     gene            complement(1764..2285)
                     /locus_tag="VHE33_03165"
     CDS             complement(1764..2285)
                     /locus_tag="VHE33_03165"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HVT96477.1"
                     /translation="MDLNGPAQPARTVAQSQSEMTELILPNDTNTLGNLLGGRLMNFI
                     DLVGAMAAYRHARSHVVTASMEHIDFIAPVHVGDLLILKSSVNRAFKTSMEVGVKVWV
                     ENTIAGIHHHVASAYLTFVAIDTQGRRVPVAPLLAETEDEKRRLEEAGRRREVREAEA
                     RRRHIPKFEPAKI"
     gene            2365..>3081
                     /locus_tag="VHE33_03170"
     CDS             2365..>3081
                     /locus_tag="VHE33_03170"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HVT96478.1"
                     /translation="MAHPHTPQPPTALPGVKSIVAIGSGKGGVGKTTLAVNLAIALAK
                     LGHRTGLIDADIYGPNVPLMLGTTRQPTVLPNNQIEPNVAHGVKVISVGFISPGDKPL
                     VMRGPMLHQIIRQFLQQVEWGELDILLVDLPPGTGDVVISLVQTVPLTGAVVVSTPSD
                     VSLQDARKALEMFHQVNVEVLGIVENMSHFTCPHCHQEIDIFSSGGAERTAKQFNIPF
                     LGSVDLVPEIRQGGDTGMPVA"
BASE COUNT          542 a          972 c          890 g          677 t
ORIGIN      
        1 taacccttaa tctggacatc gccttcctgc atgatgttga acagagccac ctgaatcttt
       61 ccagcgaggt ccggtagctc attgacggca aagatgccac gattcgcacg cggcaacagg
      121 ccatagtgca tggtcatttc gctggccaga tcatggccgc cctttgcggc tttgatggga
      181 tcgaggtcgc cgatcagatc ggccaccgtc acatccggcg tggcgagctt ctctacgtag
      241 cgttcatcgc gtgtcatcca tcccaggggc gtcgcatcgc cttgttcgac gatcaggtcc
      301 cggcagcgcc ggcacagcgg tgcataggga ttgtcgcgaa tctcgcaacc ggccacatag
      361 ggcgtgtgct cgtccaggag cgcggtcagc gcccgcagaa tgcggctctt cgcctgcccg
      421 cgcagtccca gcaggatgaa gttctgccgc gagagcaccg cgttcacaat ctgcggaacc
      481 acggtgtcct cgtatccgat gatgcccggg aatatggcct gcttcgattt gaggcggcag
      541 atgaggtttt cgcgcatctc gtctttgacg gttcgcgtgc gcagccgctg ttcgctgtaa
      601 tccgcactgg atcgcagttc acccagcgtt tgcggcagac gcactgcccg ggaatgctcc
      661 gccgtttcgg tcgtgctctg tcgagtcgcc atcgatactc ctgtcccgtg ttgctcgccg
      721 gaaattcgtg aggacttcac gttggacgcc tgaggcgcgc cgatcgtctc actgttgcta
      781 gtgtatgggg aagctggagt gccccgcctg ccctgccggc ggttttgaac tcagccgggg
      841 cagcatgtcg ttataaaagt cccgcaactt cctggtccga tccgcatcgg aacgactggc
      901 gaggagctgc tgtttgaatc ccagatccga cggcagcgca tcggcgagct gaaatgccac
      961 cggtgtgttc aggtccagat gcaacgcggg cggctggatg cctgccatgt gcagggtcgc
     1021 gaagtggagg gccgcacagt cttcgcgctc gtcccgtgta gaacctgctc cctcgtcctc
     1081 gaagaactcc acgtcagcct gcaggaacgt tcgcgactcg tcgagcgcct caatttcgaa
     1141 ccgctgcgcg ccctcacaca ggatgtccat gcgcccatcg tcgtagcgct ggacaacgcg
     1201 cacgattttc gccgtacacc ctacgatcga catttgttcc ccgtctgccc gcacgacgcc
     1261 aaacggccgg tcatcggcaa ggcagtcacc taccatctcc cgatagcgtc cttcgaaaat
     1321 gtggagcggc aatgccgcgc ctggaaacag gactgtgttg agggggaaca ggggaattct
     1381 catgatcctg acattatgct cgcctcccgc gttctcgtct tgcacaagtg gcggctactc
     1441 ggggatttcc atttcatcga gcatggcctg catctcgcga gcggcgtgct ggttgcccgt
     1501 acgtttcgca cacgccaggc cgtccaccag cttctgcttc gcctcagcgt cacggccggc
     1561 cttcgccagg gtctgagccg ccatgaagta ggcggcggtg tagtccggat gatccttgag
     1621 caattgcgta aactcggcca gggctgactc agtttggccg cgaccggaat actccatcgc
     1681 caggccgtat cgcgcaaagg cgttgctcgg atcctgctcg agaatttctt tcagtcctgc
     1741 taccttgtcc atccggcacc tgctcagatt ttcgctggct caaattttgg gatatgcctg
     1801 cgacgggcct cggcctcccg cacctcacgc cggcgccccg cctcctcgag gcggcgcttc
     1861 tcgtcttcag tttctgcgag gagcggcgcc acaggcacgc gacggccctg cgtgtcgatg
     1921 gccacaaacg tcagatacgc cgatgccaca tgatgatgaa tgccggcgat ggtgttctcg
     1981 acccagactt ttacgcccac ttccatcgac gttttgaagg cacggttgac ggaggatttc
     2041 aggatcagca gatcgcctac gtgaaccgga gcgataaagt cgatgtgctc catggaggct
     2101 gtcacgacgt gactacgcgc gtgccggtag gcagccatgg cgccgaccag atcgatgaaa
     2161 ttcatcagcc ttccgcccag cagattcccc agcgtatttg tgtcgttggg caggatgagc
     2221 tccgtcatct ctgactggga ctgcgcgacc gtgcgcgccg gctgcgcggg accgttcaaa
     2281 tccatgcgtc ttctccttct tctccagact acaggctggg ctgccctccc tgcatccgca
     2341 ggcttgtccc tgtaccctgt agatatggct catccgcaca ctccgcagcc tcccacggcg
     2401 ctgcccggcg tcaaatccat tgttgcgatc ggatccggta aagggggcgt tggcaagacc
     2461 acgctcgccg tgaatctcgc catcgcgctg gccaaactcg ggcaccgcac cggccttatc
     2521 gatgccgata tctatggacc taatgttcct ttgatgcttg gcaccacgcg ccagccgacg
     2581 gttctgccta acaaccagat tgagcccaac gtggcccatg gcgtcaaagt catttcggtg
     2641 ggatttattt cgcctggtga caagccgctt gtgatgcggg gcccgatgct gcaccagatc
     2701 atccggcaat ttctgcagca ggtggaatgg ggcgagctcg acatcctgct ggtcgatttg
     2761 ccgcctggca ccggcgatgt cgttatctcc ctggtgcaga cggttcccct caccggcgcc
     2821 gtcgttgttt ccacgccatc ggacgtctct ttgcaggacg cgcgcaaggc tcttgagatg
     2881 tttcatcagg tcaacgttga agttctgggg attgttgaga acatgtcgca tttcacgtgc
     2941 ccgcattgcc atcaggagat cgacattttc tccagcggcg gcgccgaacg cacggcgaag
     3001 cagttcaaca ttccttttct cggatccgtc gatctggtcc ctgaaattcg gcagggcggg
     3061 gataccggca tgcctgtcgc g
//
LOCUS       DASZYN010000123         1774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1159516, whole genome shotgun sequence.
ACCESSION   DASZYN010000123 DASZYN010000000
VERSION     DASZYN010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159516"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /gene="glnS"
                     /locus_tag="VHE33_03175"
     CDS             complement(<1..1106)
                     /gene="glnS"
                     /locus_tag="VHE33_03175"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HVT96479.1"
                     /translation="MAAHSESMIETPEIPTTPNFLRDRIAEDVRLKKFGDAVVQTRFP
                     PEPNGYLHIGHAKAICLDFGLADEFGGKTNLRFDDTNPEKEEQEYVDSIQKDVRWLGF
                     DWERLCYASDYFGQLYEWAIQLVRAGKAYVDDLSADEIRQYRGTLTEPGRDSPWRNRT
                     VEENLDLFTRMKAGEFPDGSRVLRAKIDMASPNINLRDPIMYRILHAEHHRTGAEWCI
                     YPMYDYAHGQSDSIENVTHSMCTLEFADHQPLYRWFIEQLGIFPSQQIEFDRLNLTYT
                     LLSKRKLLQLVQEGRVKGWDDPRMPTLSGFRRRGFTPDAIRAFVNSLGATRTNGVIDF
                     EMLEHIQRDDLNKRATRGMAVLRPLKLVIDNYPEG"
     gene            1283..>1774
                     /locus_tag="VHE33_03180"
     CDS             1283..>1774
                     /locus_tag="VHE33_03180"
                     /inference="COORDINATES: protein motif:HMM:NF024624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4034 domain-containing protein"
                     /protein_id="HVT96480.1"
                     /translation="MRLAVRTGFVAVLLTFCLVATPGSSWAQTSPVSTISADDLRQWE
                     RDLQADLTAEKFDTLDEIAGRLRRDKTRLAGGTWQLRVFYAALDVPGKTEQDSAEHIA
                     HLEHWMAQRPNSITARVALAAALVRWAWVGRGHDYADAVTPEGWRLYEERMKEAQTIL
                     EGSQ"
BASE COUNT          329 a          582 c          505 g          358 t
ORIGIN      
        1 ccctccgggt aattgtcgat caccagcttc agcggccgca gcacggccat cccgcgcgtc
       61 gctcgcttgt tcaggtcatc gcgctgtatg tgctccagca tctcaaaatc gataacgccg
      121 ttggtccggg tcgctcccag cgagttcaca aacgcccgaa tggcgtcggg cgtaaacccg
      181 cgtcgacgaa accccgacaa cgtcggcatc cgcggatcgt cccatccctt cacccggccc
      241 tcctgcacca gctgcaacag cttgcgcttc gacagcaacg tatacgtcag attcagccgg
      301 tcgaactcga tctgctgcga aggaaagatt ccgagctgtt cgatgaacca ccgatacaga
      361 ggctgatgat ccgcaaactc gagcgtgcac atcgagtgcg tgacattttc gatcgagtcc
      421 gactggccgt gtgcgtagtc gtacatcggg tagatgcacc actcggcgcc cgttcggtga
      481 tgctccgcat gcaggatgcg atacatgatg gggtcgcgca gattgatgtt cggcgacgcc
      541 atatcgatct tcgcccgaag cacgcggctc ccgtcgggaa actcacccgc cttcatgcgc
      601 gtgaacagat ccaggttttc ctccaccgtg cggttgcgcc acggactgtc cctgcccggt
      661 tcggtcagtg tcccgcgata ctgccggatc tcgtctgccg acaagtcgtc cacataggcc
      721 ttccccgccc tgaccagctg aatcgcccac tcatagagct ggccgaagta atccgacgca
      781 tagcatagcc gctcccagtc gaacccgagc cagcgaacgt ccttctggat cgaatcgacg
      841 tactcctgct cctccttctc cggattggtg tcgtcaaagc gcaggttggt cttgccccca
      901 aactcatccg ccagcccgaa gtccaggcag atggctttgg catgaccgat atgcaggtag
      961 ccgttcggct ccggtggaaa gcgcgtctgc accactgcat cgccaaactt cttcaggcgc
     1021 acatcctccg cgatcctgtc gcgaagaaaa ttcggtgtcg tgggaatttc gggcgtttct
     1081 atcatggact ctgaatgggc tgccattctc ctattcttcc acggcagggg gccgtccccg
     1141 ctgccaggag gtcctgaaca tctcagttct gcgggtcggc cgaaggaaca ttcagcttca
     1201 aagccaaggt tgcctcctgg acagagacgc gttaccctgt ggggctcctg ccgtttgaga
     1261 atgcggagcg ctctctatgc ccatgcggct cgctgtccga actggctttg ttgctgttct
     1321 cctgaccttc tgcctcgttg cgacgcccgg ctcttcctgg gcgcagacat ctcctgtcag
     1381 cacgatttcc gcggacgatc ttcgccaatg ggagcgtgac cttcaggcgg acctgacggc
     1441 agagaaattc gacaccctcg atgagattgc cggccgcctg cgcagggaca agacccggct
     1501 tgccggcggc acgtggcagt tgcgcgtctt ctatgccgcc ctcgatgtgc cggggaagac
     1561 cgagcaggac agcgcggagc acatcgcgca tctggaacac tggatggccc agcgccccaa
     1621 ctccatcacc gcgcgggtcg ccctcgcagc ggcacttgtt cgctgggcgt gggtcggccg
     1681 cgggcacgac tatgccgacg cagttacacc ggagggatgg cggctctacg aagagcgcat
     1741 gaaggaagcg cagaccattc tcgaaggctc gcag
//
LOCUS       DASZYN010000124         6083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_645398, whole genome shotgun sequence.
ACCESSION   DASZYN010000124 DASZYN010000000
VERSION     DASZYN010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6083
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645398"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VHE33_03185"
     CDS             <1..217
                     /locus_tag="VHE33_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HVT96481.1"
                     /translation="LKIVALIVQGCKNKEIAVRLNTTEQVIKNYLRSVYDKTGVSDRL
                     ELALFTIHHRILAEAAQAIGDQMLMGV"
     gene            422..1615
                     /locus_tag="VHE33_03190"
     CDS             422..1615
                     /locus_tag="VHE33_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96482.1"
                     /translation="MHSRFGTAERKTAYNPASIMGARFIPVFVLFIAIPRAHGVKPVT
                     IAQMGEILDANRGRSDTRVAGEIAGFKLTERVSARQFAAWEAQFPGPQTQDRLLVLSD
                     ESAFQPLPASEISADPSPALSAEEKILDAARIYASKTMRNLPNLLAIRTTVHFQSSSD
                     DALKRARENPVGSEGVDDLPLSFTGQSSAPIAYRDGNEVAVDHGRMPGSHGLTTRDEF
                     GPILTIVLGDAKQGSIVWGYWQRNGNTRIAVFRYRVPAEKSHYLVNFPEGGLIRLRPA
                     YHGEIAIDPETGSILRLSIVSDPKRLPEPAIGVVVEYGSVNLGDRPYIVPLHGIALGK
                     VPIGSIPRQDGSAITPVQTFLNDIAFRSYQLFRADSRILSPEESRQLREEVPAAGSPG
                     SPQHP"
     gene            complement(1760..3352)
                     /gene="pruA"
                     /locus_tag="VHE33_03195"
     CDS             complement(1760..3352)
                     /gene="pruA"
                     /locus_tag="VHE33_03195"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF002852.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HVT96483.1"
                     /translation="MATLESSTVILPRKPQGAFRNEPFIDFTQHDNARAMRGALERVG
                     DQLGHEYPLIIGGERLKTSAKIESLNPARPAQIVGVHQKAGAEHAEQAVQAALKAFET
                     WRFAPVEERASLLFGAAEIIRERKFEFCAWLTYEVGKNWAEADADVAETIDFLEFYAR
                     EALRLAASTPPIQYPGERDELLYIPLGVGAVISPWNFPFAIMAGMTSAAIVSGNTVVL
                     KPSSDSPTIAARFMEVLEEAGLPAGVVNFCPGSGASFGNAVVAHPKTRFIAFTGSKEV
                     GLDIHERAAKTQPGQIWIKRTILEMGGKDSIIVSADADFDAAVAGVVASAFGFSGQKC
                     SACSRAIVEEPIYDAFVERLREKVAELKVGDPAGNANMGPVVSEGAMKTILNYIEIGK
                     KEGRVVSGGAMVETPEKGYYVQPTVIADVAPKARIAQEEIFGPVLAVIKARDFEDALA
                     IANNTEFGLTGSIYSTDRVKLNRARQEFHVGNLYFNRKSTGAMVGAHPFGGFNMSGTD
                     SKAGGPDYLYLFTQAKAVGEKL"
     gene            complement(3423..5189)
                     /locus_tag="VHE33_03200"
     CDS             complement(3423..5189)
                     /locus_tag="VHE33_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96484.1"
                     /translation="MRFSSVSLGNKFTAVLLAAAAGYGAHIWLQRRAVETAATAQLSF
                     SLHAARRLDAGYVHASEPAIVFANSVLSDQRIADLSKPSYLSTSAMMSRVGEFRSRLE
                     LTQPSPQVLGVRFRDADAAKAIATANQVANALVAWSPSPDAPGTLTEPTAPAQPAAQQ
                     PPAAKAAPAPAAVPAKKPVTATSNSEDSASAGPLVAALDQLQKQLTSTSHQVEEGGSS
                     SRGHAYAQSEQQHLLKAQVLEAEKKVADLRAQYGNDGTGQHLGAIQQALGSVFSRGSV
                     GVSASQLRREREGLAHAIAVVQTQRAAIEKEGGAGTEPSAATPAPASTSANDSNTPTA
                     KGSGSSAISESDLTKAGTPAPASQTSTAAGSQGASSSSTASEPIEDPSLANPFRMEKA
                     AGVAAPVQWWPAAAVGVGCGLLYLLLMAMISGGREDETVYAEDSVSFGRFITSDGPAP
                     AIVPTPEPEPEPAPVADRGQNRRASFTWDPGPLGNAARAENTVAVEPAPASEAENAPA
                     IQPNESRAVAESDESSTARGAFHQDKVTEKVVDVDPWADLMQKALSETDIGRKFETPK
                     ERNESAKDAGSLQPSRSGRWAS"
     gene            complement(5469..>6083)
                     /gene="msrA"
                     /locus_tag="VHE33_03205"
     CDS             complement(5469..>6083)
                     /gene="msrA"
                     /locus_tag="VHE33_03205"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HVT96485.1"
                     /translation="ASAAGRAPIPAAHADVALAAHSGKAKAVFAGGCFWGVQSVFERV
                     KGVVDTTAGYTGGSAATATYDQVTTETTGHAESVEVVYDPSKITYGQLLRIFFSVVHD
                     PTELNRQGPDVGTSYRSAVFYTSEEQKRIAQAYIAQLDAAKIFPRAIVTEVTPLKGFY
                     RAEDYHQDYALHNPDNPYIQVCDRPKVEALRQQFPELFREYKGH"
BASE COUNT         1121 a         2091 c         1691 g         1180 t
ORIGIN      
        1 actgaagatc gtcgcgttga ttgtccaggg ctgcaagaac aaggagatcg ccgtccgcct
       61 gaacaccacc gagcaggtca tcaagaacta cctgcgcagt gtttacgaca aaaccggcgt
      121 ctcggatcgc ctggagctgg ccctgttcac catccaccat cgcattctgg ccgaagccgc
      181 acaggccatc ggcgaccaga tgctgatggg tgtttagaac agactccgct tcctggcaga
      241 atcgcagacc taagaggcgc tgccgttgtg cgcagcgcct ccggtttcac ggaagctgta
      301 aggatgaggc aggagccccc gcatctttgc ttccgggagc gcctgcaccc ggctccgctc
      361 gggccgaagc gaaacgtccg cttgtccagc accgccggtc acatcgttgc gaagctctcc
      421 gttgcacagc aggttcggaa cagccgagcg gaagaccgcc tataatcccg ccagcatcat
      481 gggcgcgcgc ttcatccccg tattcgtgct ttttattgct attcctcgag cacacggcgt
      541 taagcccgtc acgattgctc aaatggggga aattctcgac gcgaaccgag ggcgttctga
      601 tactcgcgtc gccggcgaaa tcgccggttt caagctcacc gagcgagtga gcgcccggca
      661 gttcgctgcc tgggaggcac agttccccgg ccctcagacc caggacaggc ttctggttct
      721 ttcagacgaa tcggcatttc agccgctgcc tgcctccgaa atatcggccg atccaagccc
      781 cgccctttct gccgaagaaa aaatcctgga tgcggctcgg atctacgcca gtaaaaccat
      841 gcgcaatctt ccgaatcttc ttgcgatccg aacgacagtg catttccaga gttcctccga
      901 tgacgcatta aagcgggccc gcgagaaccc agttggatcc gaaggagtcg acgatctgcc
      961 gctgtccttt accggccagt caagcgctcc catcgcatat cgcgatggca acgaggtggc
     1021 tgtggatcat ggacggatgc caggcagtca cggtctcacc accagagacg agttcgggcc
     1081 catccttacg attgttctcg gtgatgcaaa acagggctcg atcgtctggg gttactggca
     1141 gcggaacggc aatacccgca tcgctgtctt tcgatatcgc gtgcctgcag aaaaatccca
     1201 ttacctggtc aattttcctg agggcggcct catccgattg cgtcccgcct accatggcga
     1261 gattgcgatt gatcccgaga ctggaagcat tctccgcctc tccattgttt ccgaccccaa
     1321 aaggcttccg gagccggcga tcggcgtcgt ggttgaatac ggctcggtga acctgggtga
     1381 ccggccctat atcgtgccgc ttcacggcat tgccttaggc aaggtgccca tcggttccat
     1441 tcctcgacag gatggctcgg ccatcactcc ggtgcagacc ttcctgaacg acattgcctt
     1501 caggagctac caactgttcc gcgctgactc tcgcatcctg agccccgagg aatcgagaca
     1561 actccgtgag gaggttcccg cagcgggatc tccaggttca ccgcagcatc cttaaatcgt
     1621 cacttttgtc aggaacactc ctggcgtgac ccgggccgcc aacgtcgaaa acgatgctga
     1681 gccgattgcc aaatcggccg tgacacggtt tcccgaacac tcggcctgtg actcgcgacc
     1741 tgcgctctga acccccgagc tacagcttct cccccacggc cttcgcctgc gtgaacaggt
     1801 acaaataatc cggcccgccc gccttcgagt ccgtgccgga catgttgaac ccgccgaacg
     1861 gatgcgcgcc caccatcgcc cccgtgctct tgcgattgaa gtacaggttc cccacgtgaa
     1921 attcctgccg cgcgcgattc agcttcacgc ggtccgtcga gtagatcgaa cccgtgagcc
     1981 caaactccgt gttgttggcg atcgccagcg catcctcgaa gtcgcgcgcc ttgatcaccg
     2041 ccagcaccgg cccgaagatc tcttcctgcg caatgcgcgc ctttggcgcc acatccgcaa
     2101 tcaccgtggg ctgcacgtag tagcccttct ccggcgtctc caccatcgcc ccgccggaaa
     2161 ccacgcgtcc ttccttcttc ccaatctcga tgtagttcag gatcgtcttc atcgcgcctt
     2221 cgctgaccac cggccccatg ttcgcgttcc cggcgggatc acccactttc agctccgcga
     2281 ccttctcccg cagccgctcg acgaacgcat cgtagattgg ctcctcgaca atcgcccgcg
     2341 agcacgccga gcacttctgc ccgctgaatc cgaacgccga agccaccaca cccgccaccg
     2401 ccgcgtcgaa atccgcatcc gcgctcacga tgatcgagtc cttgccgccc atctccagta
     2461 tcgtccgctt gatccagatc tgccccggct gcgtcttcgc cgcccgctcg tgaatgtcga
     2521 gcccgacctc cttcgatcct gtgaacgcga tgaagcgcgt cttcggatgc gccaccacag
     2581 cattcccaaa cgacgcgccg gaccccggac aaaaattcac aacacccgcc ggcagccccg
     2641 cttcttccag aacctccatg aagcgcgccg cgatcgtcgg cgaatcgctc gagggcttca
     2701 gcaccaccgt gttcccgctc acaatcgcag ccgacgtcat ccccgccatg atggcgaagg
     2761 ggaagttcca gggcgaaatc accgcgccta cgccgagcgg aatgtagagc agctcatcgc
     2821 gttcgcccgg atactgaatc ggcggtgtcg aagcggccag gcgcagggct tcgcgcgcgt
     2881 agaactccag aaagtcgatc gtctcggcga catcggcgtc cgcctccgcc cagttcttgc
     2941 cgacctcata ggtgagccac gcgcagaact cgaacttgcg ctcgcgaatg atctccgccg
     3001 caccgaacag cagcgacgcg cgttcttcga ccggcgcaaa tcgccacgtc tcaaatgcct
     3061 tcagcgccgc ctgcacggcc tgctcggcat gctccgctcc ggccttctga tgcacgccga
     3121 caatctgcgc cggccgagcg gggttgagcg actcgatctt tgcgctggtt ttgagccgct
     3181 ccccgccgat gatcagcgga tactcatgcc ccagctgatc gccgacccgc tccagcgcgc
     3241 cacgcatggc gcgcgcattg tcgtgctgcg tgaaatcgat gaacggctca ttgcggaacg
     3301 cgccctgtgg cttgcgcggc aggataacgg tggagctttc aagggtagcc ataacagctc
     3361 agtttagttc aaaccgaggc tggcgatggt ttctgttggc tactggctac tccctgccgt
     3421 tttcagctcg cccagcgacc cgagcggctc ggctgcagcg atccggcgtc cttcgccgac
     3481 tcgttgcgct ccttcggtgt ctcgaatttg cgcccgatgt ccgtttccga aagggccttc
     3541 tgcatcagat ccgcccacgg gtccacgtcg acgaccttct ccgtcacctt gtcctgatgg
     3601 aacgcccccc gggcggtcga gctttcatcc gattcggcca cagcgcggct ttcattcggc
     3661 tggatcgcag gagcgttctc cgcctcgctg gccggcgcag gctcaaccgc aacggtattc
     3721 tccgctcgcg ccgcatttcc cagcggaccc ggatcccagg taaacgacgc cctccggttc
     3781 tgacctctgt ccgcgaccgg cgccggttcc ggctcaggct caggcgtggg cacaatcgcg
     3841 ggcgccggcc catccgatgt aatgaatcgc ccaaacgaca cgctgtcctc ggcatagacc
     3901 gtctcatcct cacggccacc ggagatcatc gccatcagga gcaagtagag caacccgcat
     3961 cccacgccca cagccgccgc gggccaccac tgcactggcg cggcaacgcc ggcggctttt
     4021 tccatccgaa atggattggc taaggacgga tcctcgatcg gctcggatgc tgtggaagac
     4081 gaagaggctc cctgcgaacc agctgccgtg gaagtctgcg aagcaggcgc cggcgtaccc
     4141 gctttcgtca aatcgctttc cgaaatggcg ctcgaacccg aacccttcgc tgttggcgta
     4201 ttgctgtcgt tcgcgcttgt cgaagctggc gctggcgtcg ccgcactcgg ctccgtaccc
     4261 gcgccgcctt ccttctcgat cgccgcgcgc tgggtctgca ccacggcgat cgcatgcgcc
     4321 agtccctccc gctcacggcg caactggctc gcgctcactc caacgctgcc ccgtgagaaa
     4381 acagagccga gcgcctgctg gatagcgccc agatgctgtc cagttccgtc attgccatat
     4441 tgcgcccgca gatcggcgac cttcttctcg gcctcaagga cctgcgcctt gagcaggtgc
     4501 tgctgctccg attgtgcgta ggcgtggccg cgcgacgaac tgccgccttc ctctacctgg
     4561 tggctcgtcg aggtcaactg cttctgcaat tgatcgagag cggccacaag aggcccggcg
     4621 cttgccgaat cctcactgtt gctcgttgct gtgaccggct tcttcgccgg tactgcggcc
     4681 ggagccggcg ccgcctttgc tgctggtggc tgctgggctg ccggttgcgc gggagccgtt
     4741 ggctcggtca atgtgccggg ggcgtccggc gaagggctcc acgccaccag agcattggct
     4801 acctgattgg cggtcgcgat cgccttcgcc gcgtccgcat cccggaatcg cacacccaga
     4861 acctggggtg agggttgcgt cagttccagg cgcgagcgaa actcgccaac ccggctcatc
     4921 atcgcggatg tcgacagata cgacggcttc gaaagatccg cgatcctctg atcgctcagc
     4981 accgagtttg cgaagacaat ggccggctcg ctcgcatgga cgtatcccgc atcgagccgg
     5041 cgcgccgcgt gaaggctgaa ggacaactgt gcggtcgcgg ccgtctcgac cgcccgtcgt
     5101 tgaagccaga tgtgagcccc gtagccagcc gctgcggcca gcaatactgc agtgaacttg
     5161 ttccccaggg aaacagaaga aaaacgcata aaactcctct cgcatcgtgc atgaacgccg
     5221 ccgaacgtat cggagaccga ggcgatttct tcccggcgaa gcaaaggctg gcgcgactta
     5281 caaattgcag ggcccaacct ggattggaag aaagaatgag gccggtcgtt gtctggcgtg
     5341 cctgatctga attccgcgat gcacgcaaac ttctggtacc aaaacaactt acttcacgat
     5401 acgccgggac gcacagaggc gccaccccgt gccacgaacc gggttcggat cccgagccta
     5461 gcttgccgtc agtggccttt atattccctg aataattccg ggaactgctg gcgcagcgcc
     5521 tcgaccttcg gccgatcgca cacctgaatg tacggattgt caggattgtg cagcgcgtag
     5581 tcctggtggt aatcctcagc gcgatagaag cccttcagcg gcgtcacctc agtcacaatc
     5641 gcccgcggaa atatcttcgc cgcatcgagc tgcgcaatgt aggcctgcgc gatgcgcttc
     5701 tgctcttcgc tggtgtaaaa aaccgccgac cggtacgacg tgccgacatc cggcccctgc
     5761 cggttcagtt ccgtcggatc atgcacgacc gagaaaaata tccgcagcag ctgcccatag
     5821 gtgatcttcg acgggtcata gaccacctcc accgactccg cgtgtcccgt tgtctccgtt
     5881 gttacctgat cgtaggtcgc ggtcgcagcc gatccgccgg tatagcccgc ggtcgtatcg
     5941 accacgcctt tcactcgttc gaagaccgac tgcacgcccc agaagcatcc cccggcaaag
     6001 accgccttcg ctttgcccga atgagccgcg agcgcgacat ctgcatgcgc agcaggaatc
     6061 ggcgcgcggc ccgcagcgct ggc
//
LOCUS       DASZYN010000125        11281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_5602, whole genome shotgun sequence.
ACCESSION   DASZYN010000125 DASZYN010000000
VERSION     DASZYN010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 11281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11281
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5602"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="VHE33_03210"
     CDS             complement(<1..1347)
                     /locus_tag="VHE33_03210"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HVT96486.1"
                     /translation="MTRVALPLHETPGEPPIEWTLVDLVAILVRRRAWILASLALCCG
                     TAFLYWLSATPRYRATAIIEVHKDSHGAFGLDNTTADRPSNETNDSFDDNLTLQTEIG
                     ILQSDALTLDVVRRLQLETTPDYSALHSRPSGVARKLFFWRPPVEPLTVPLADAPNRR
                     FAALRIFARHRRIATQPGTRLIAISYSDPSPERAAIIANGIVQALSDYGFQSRSAAAA
                     QSASWLTTQLTDLKQQTDALDARATALDRASGDFGDDGAHNPVLARLDSLNSALSAAE
                     SSRIVREAIWRTVQRGDPEAISGLAGNPDAGTNTQNSLALLQSLRAQESTLKSQIAES
                     SNRYGANWPAFAEQRAHLESIQNSIQEEVRRLADRARTDFEVSLQAENSARDAFTQQK
                     NLASQLTGNAVALRLARQEAGESRALYSSLLGRLQQTGVLEGLHSANFAVVSPALIP"
     gene            complement(1375..2358)
                     /locus_tag="VHE33_03215"
     CDS             complement(1375..2358)
                     /locus_tag="VHE33_03215"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96487.1"
                     /translation="MTRTPLRISIGGGGTDLPSYYQENGGGFVISAAINKYVYISMNR
                     SFLPGYFLKYSETEHTDTCDQIRHPLMREALRTHRAPAPLEVVSVADVPAGTGLGSSG
                     TFLVGLLHAIYAWQRQPVTAEALAREAIDIEMNRLGEPVGKQDQYIAAYGGLLCQEYL
                     PDGDVRVTPLRMPEESLRELRDSLMLFFLGELRSASSLLGEQKRLSEARNPAMIDSLH
                     FARMLGREIRSVLEAGQVAEFGSLMHQHWMRKRNRARNMSSSRIDSLYDLARSDGGAT
                     GGKLVGAGGSGFLLLHTQDRRRLRDTMLRAGLTEMDFSFDFDGSVVLLRNG"
     gene            complement(2367..3164)
                     /locus_tag="VHE33_03220"
     CDS             complement(2367..3164)
                     /locus_tag="VHE33_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HVT96488.1"
                     /translation="MKDYLHRESLWPPRLALLVGGLATRLRPLTSFMPKSLIRIAGEP
                     FLAHQLRLVRAGGIREVVLCCGFLGDQIEDFAGDGSRFGLSITYSHDGEQPLGTGGAL
                     LKALPLLGERFLVMYGDSWLTEPIEPLWRAFLDSGKPAMMTVFRNENRWGASNVEFRK
                     GSVVRYDKRHPSPAMHHIDYGLDALDTSVLAHWSTPSFDLGDVWSGLADYSLLAGYET
                     SERFYEIGSLAGMRETESVVAASPRVRRVLSFARQSAPPPQRDTQVV"
     gene            complement(3250..4224)
                     /locus_tag="VHE33_03225"
     CDS             complement(3250..4224)
                     /locus_tag="VHE33_03225"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVT96489.1"
                     /translation="MLLSEGSYFIVGGAGFIGSHFVDALLRSDMTRRVTVFDNFSSGR
                     EWHCEPHARNPRLKIIRADASDVHALTATMEGHDWVIHLASNPNIAAAAENPEIDFYR
                     GTLLTNNVVEAMRRTGVHRLLYASGSGVYGDTGSQSVVEDHAPLIPVSTYGASKLACE
                     SLIASYCSMFGLRACAFRFGNVVGPRQTHGVGFDFLRSLSAKPKRLRILGDGNQSKPY
                     IHVTDVVDAAFCAAKLARGAFSVFNVATDDAVTVTEIAELAVATLDLDRPTFEYTGGS
                     RGWRGDVPVVRLDSTRIRSLGWFPKRNSREAMRQSLQELIADDRILHN"
     gene            4542..5546
                     /locus_tag="VHE33_03230"
     CDS             4542..5546
                     /locus_tag="VHE33_03230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HVT96490.1"
                     /translation="METREAGLTVGMPVCNGMPWLPEAMDSLLQQTTKAFQILVIVDG
                     GSDDSATWLRNLENRLARVGFPWPRLRVLEQEHAGITATLNRLLYETRTPWLVRMDAD
                     DVSYPTRLERLQIAIEEHSDAGLIYSLADYYPQDRCAGRFRCSRGTPEELQAMVRSGY
                     LLSICHSSVALNVEKVRSVGGYRMNLHAEDADLWWRLALRYKIHFIPEPLVGFRIHPR
                     SVSSSNLETQQLGGVYVQYLLLSELWGKKPRAFGEIAEALGEFVQPAAFEAKEALRKF
                     NTLLAEKRYVRGLGALAHAFHASPGYVSRRVREEFRPAAIGNGVAPAKFWQRKEELWA
                     "
     gene            5537..6457
                     /locus_tag="VHE33_03235"
     CDS             5537..6457
                     /locus_tag="VHE33_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HVT96491.1"
                     /translation="MGVIGDLSAPGTDAGTETAARLPFPPRFLLGRIPVDRITMDYAA
                     VLLVEALLHRGALPPLTVVGPNAHLVTMAERDSRFAEAMCDADLAVPDGVSVVMASRF
                     LGFPIPERVTGGDLMERMCAEAAHYGFRVFFLGGLLGAAAMAAHHLCSRYPGLQICGT
                     YCPPVGFEHDPSEMERMRRAIADATPDLICVAFGAPKQEIWMQQNREQFHVGAMMAVG
                     AALDTQAGLRRRAPRWIQAIALEWLFRLAMEPRRLWRRYLIGNAEFVLLVLGQWMRER
                     WAGLRRWLAPAIAIRETENPGSTGMGHEED"
     gene            6444..7301
                     /locus_tag="VHE33_03240"
     CDS             6444..7301
                     /locus_tag="VHE33_03240"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HVT96492.1"
                     /translation="MKKIEDLRIRLFSDGADFAGIMEMYSRPWVRGFTTNPTLMRKAG
                     VRNYEAFARSLLRAIPDRPVSLEVFADDEEQMEMQALTVATWGANVNVKIPVTNRAGV
                     LQTRLIRTLSEAGVAVNVTAILTLEQVEQVVGALAAETGAIVSIFAGRIADTGVDPVP
                     VMAEAKRILRWRPRAQLLWASPREVLNVFQAEDAGCDIITLPPEMLRKLELVGRDLKQ
                     YSLETVEMFHHDARAVGYVIDTSGLAAAKASGQPAGRTVENNFGRFREITCDVVGAGI
                     GSASTGVEA"
     gene            complement(7790..8386)
                     /locus_tag="VHE33_03245"
     CDS             complement(7790..8386)
                     /locus_tag="VHE33_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96493.1"
                     /translation="MDYTSVYLSEVAALAAHLDTTVIDRMIDLLHEVRAQGGRLFFLG
                     VGGGAGHAGHAVNDFRKIAGMECYTPTDNVSELTARINDDGWETCYANWLRGSRLSSR
                     DAVFIFSVGGGSVERHISTNLVASLNLAREAGARILGVVGRDGGYTAQLADACLIVPT
                     LSPETVTPHTEEFQAVIWHLIVSHPRLRANPMKWESAR"
     gene            complement(8407..9441)
                     /locus_tag="VHE33_03250"
     CDS             complement(8407..9441)
                     /locus_tag="VHE33_03250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVT96494.1"
                     /translation="MSDSFPGASYPIVSAVIPTHGRPRLLACAVRSALRQTWSPIEVI
                     VVVDGPDPATVLSLKAITDPRVRVVVLPEPCGGSEARNVGVRVARGDWIAFLDDDDEW
                     LPEKIERQMHAAHAMPDWFPVVSCRLIAQSPSVSRVLPEQLYEAPRAIADFLFCRASL
                     RDPGGVMQTSTLLAPRDLLIAVPFRAGLPMHQDWDWLIRVASYKGVGLSMVRQPLAIW
                     RVEDSRSTVGRDPAWKNSLSWIRSVRSLISPRAFSWFVAIQCGWRAQASRAGWLAKFR
                     LLHAFLQEGAPEFRSLVSFLIFAFVPVRVRRFFRAGFLRKRAASDPAPGLRLVFSRKT
                     STPILRRSSS"
     gene            complement(9648..10412)
                     /locus_tag="VHE33_03255"
     CDS             complement(9648..10412)
                     /locus_tag="VHE33_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HVT96495.1"
                     /translation="MKSQVIGTTMPVLEFLLEPNDAIISEAGELSWMSANVQMTTHTQ
                     FAGGGGIFGAIKRVAGGGSLFMTEYRAAGGNAELAFATRVPGHIVPVEVVPGYDYMVH
                     RHGFLCATAGVQIGVGFQQSLGAGIFGGDGFLLQKISGQGLAWLELSGEVVTRDLQQG
                     ETLLVHPGHVGAFTASVSFQITMVRGIKNLIFGGDGLFLASMTGPGRIWLQTLPISRL
                     AHQLQEYMPAGREVRQSTEAGLLGGIVGSVFDNMNR"
     gene            11048..>11281
                     /locus_tag="VHE33_03260"
     CDS             11048..>11281
                     /locus_tag="VHE33_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96496.1"
                     /translation="MNLNIFPSMFGRSVTAVDAACCTHPSLGKTLPARKRLANRYSKV
                     VFAGVALAILCCTPGSLSAQNHRWHRWGRWGGSG"
BASE COUNT         2314 a         3012 c         3904 g         2051 t
ORIGIN      
        1 aggaataaga gccggactga cgacggcaaa gttggcggaa tgaagccctt caagaacgcc
       61 ggtctgctgg aggcggccga ggaggctgct gtaaagcgca cggctctcgc cggcttcctg
      121 acgggcgagg cgcagcgcaa cggcattgcc tgtgagctgc gaggcaaggt tcttctgctg
      181 cgtgaaggcg tcgcgggcgg agttctcggc ctgcagggag acttcgaagt cggtgcgggc
      241 gcggtcggcg aggcggcgga cttcttcctg gatggaattc tggatgcttt cgaggtgagc
      301 gcgttgctcg gcgaaggcgg gccagttcgc accgtagcga ttggatgact cggcgatctg
      361 acttttgaga gtggactcct gagcgcggag agactgcagg agggcgaggg agttctgggt
      421 gttggtgccg gcatcgggat tgccggcgag tccggagatg gcttcgggat cgcccctctg
      481 gaccgtgcgc catatcgctt cgcgcacgat ccggctggat tcggcggcgg aaagagcgga
      541 gttcagcgag tcgagacggg ccagcacggg attgtgtgcg ccatcatcgc caaaatcgcc
      601 ggaagcgcgg tcgagggcgg tggcgcgggc gtccagagcg tcggtctgct gtttgagatc
      661 ggtgagctga gtagtaagcc aggaggcaga ttgtgcggcg gcggcggagc gcgattgaaa
      721 gccatagtca gaaagcgcct gaacgatgcc attcgcgata atggcggcgc gttcggggct
      781 gggatcggaa tagctgatgg cgatcagacg cgtgccgggt tgcgtagcaa tcctgcgatg
      841 ccttgcgaag atcctgagcg cggcaaagcg gcgattgggc gcgtcggcga gcggaacggt
      901 gagcggttcg accgggggac gccaaaagaa aagcttgcgg gcgacaccgg atggcctgga
      961 gtgcagagca gagtagtcag gcgtggtttc gagttgcaga cggcggacga cgtccagggt
     1021 gagcgcgtcg gactgcagga tgccgatttc ggtctggaga gtgagattgt cgtcgaagga
     1081 gtcgttggtt tcgttggagg gacgatccgc cgtggtgttg tcgaggccaa aggcgccgtg
     1141 ggaatccttg tggacttcaa tgatcgcggt cgcgcggtac cgcggcgtgg ccgaaagcca
     1201 gtacagaaag gctgtgccac aacacagtgc gagggaagcg aggatccagg cgcggcggcg
     1261 caccaggatg gcgacgagat cgaccagagt ccactcgatc ggcggctcgc caggagtctc
     1321 atgcagcggc agagcaacgc gcgtcatggc ttcggctccc gaactgaagt gggtttagcc
     1381 attgcgaagc agtaccaccg atccgtcgaa gtcgaagctg aagtccatct cggtgagtcc
     1441 ggcgcggagc atggtgtcgc gaaggcggcg gcggtcctgg gtgtgcagca ggagaaagcc
     1501 gctgccgccg gcgccgacga gtttgccgcc ggtggctccg ccgtcggagc gggcgagatc
     1561 gtagagcgag tcgatgcggc tgctggacat gtttcgcgct cgattgcgct tgcgcatcca
     1621 gtgttggtgc atgaggctgc cgaattcggc gacctgacca gcttcgagga cgctgcgaat
     1681 ttcccgtccg agcatccggg cgaagtgcag gctgtcgatc atcgctggat tgcgcgcctc
     1741 ggagagacgc ttctgctcgc cgagcagcga tgatgcgctg cgaagctcgc cgagaaagaa
     1801 aagcatgagg gaatcgcgga gttcgcggag ggattcttcc ggcatgcgca gaggcgttac
     1861 gcggacgtca ccgtcaggaa gatattcctg gcagagcagg ccgccataag cggcaatgta
     1921 ctgatcctgc ttgccgaccg gctcgccgag gcgattcatc tcgatgtcga ttgcttcccg
     1981 ggccagcgct tctgcggtga cgggctggcg ctgccaggcg taaatggcgt ggagaagacc
     2041 cacgaggaac gtgcccgaag aaccgagccc ggtgccggcg gggacgtcgg cgacactgac
     2101 gacctcgagc ggcgcaggtg cacggtgcgt acggagcgcc tcgcgcatca ggggatgacg
     2161 gatctgatcg caggtgtcgg tgtgttcagt ttcggagtac ttcaggaaat agccgggcag
     2221 gaagctgcga ttcatcgaga tatagacata tttattgatg gctgcagaga tgacgaagcc
     2281 tccaccgttc tcctggtaat acgaagggag gtccgtgcca ccgccgccaa tggagatccg
     2341 caggggtgta cgcgtcatga tcaacgtcac accacctgcg tatcccgctg cgggggaggg
     2401 gcgctctgcc ttgcaaaaga gaggactcgg cgcacgcgcg gagaagcggc gacgacggac
     2461 tcggtttcgc gcatgccggc gagggatccg atttcgtaga agcgctcgga ggtttcgtat
     2521 ccggcgagaa gcgaataatc ggcgaggccg ctccacacgt cgcccaggtc aaaggatgga
     2581 gtgctccagt gggcgaggac gctggtgtcg agcgcgtcga ggccgtagtc gatgtggtgc
     2641 atggcgggag agggatggcg tttgtcgtag cggacaacag aacctttgcg gaactcgacg
     2701 ttgctggcgc cccatcgatt ctcattgcgg aagacggtca tcatggccgg cttgccggag
     2761 tccagaaagg cgcgccagag gggctcgata ggctcggtaa gccagctgtc gccatacatg
     2821 acaaggaagc gctcaccgag cagcggcagg gccttgagga gagcgccacc agtgccgagc
     2881 ggctgttccc catcatggct gtaggtaatg gacaggccga agcgactgcc atcaccggcg
     2941 aagtcctcaa tctggtcgcc gaggaaacca cagcagagca cgacttcgcg gatgccgcca
     3001 gcgcgaacga gacggagctg atgcgccagg aacggctcgc ccgcgatccg gatgagcgat
     3061 ttgggcatga atgaggtgag gggacgcagc cgggtcgcca ggcctccgac aagcagggca
     3121 agacgcgggg gccagaggga ctctcgatgg aggtagtcct tcatgatctg gcgtcggttg
     3181 ctggtggaat cagagaaagg cgcagggccg cacgccccca tggccaacac ggaaggagac
     3241 gggccccagt cagttgtgaa ggatgcgatc atcggcgatc agctcctgca gggattggcg
     3301 catggcttcg cggctgttcc gttttgggaa ccagccgaga gagcggatgc gagtggaatc
     3361 gagacggacg accgggacat ctccgcgcca gccgcgggat ccgccggtgt attcaaaggt
     3421 gggccggtcg agatcgagag ttgcaacggc cagttcggcg atttcggtca ccgtgacagc
     3481 atcgtcggta gcgacattga agacagaaaa ggcacccctg gccagctttg cggcacagaa
     3541 ggcggcgtcg acaacatcgg tgacgtgaat gtaaggcttg ctctggttac cgtcgccaag
     3601 gatgcgaaga cgcttgggtt tggcactgag gctgcggagg aaatcgaagc cgacgccgtg
     3661 ggtctgccgg ggtccaacca cattgccgaa acggaaggca caagcgcgaa ggccgaacat
     3721 cgagcaatag ctggcgatca gggattcgca ggcgagctta cttgcaccat aggtggagac
     3781 aggaatgagg ggtgcgtggt cctcgacgac ggattggctg cccgtatcac cgtaaacgcc
     3841 gcttccggag gcgtagagga ggcgatggac gccggtccgg cgcatggctt cgaccacatt
     3901 gttcgtgagc agggtgccgc gataaaaatc gatctccggg ttctcggcgg ccgctgcgat
     3961 gttgggattg gaggcgagat gaatgaccca gtcatggcct tccatggtgg cagtgagggc
     4021 gtgaacatca ctggcgtcgg cgcggatgat tttaaggcgt gggttgcgag cgtgcggctc
     4081 gcagtgccac tcgcgcccgg acgagaagtt gtcgaagacg gtgacgcggc gggtcatatc
     4141 cgacctcagg agcgcgtcaa cgaaatggct gccgatgaag ccggcgcctc caacaatgaa
     4201 atagctgccc tccgaaagga gcattttgat cctctgctga ttacgaacga gtgctgctga
     4261 ttccgttgtg aaactggctg gggctttctg acccagaatg aaactgcgag ctctatgttt
     4321 gagtgcgaac gaggcatatg gcaacagttt ctgtgattgg ggacattaca aaggtcagag
     4381 ttataagaag gttatggcct gttcgcgcca ttacctgggg catgggagaa ccgcaatcca
     4441 caggacgaag ttaactccgt cccatttata agtggctccg tagttgtccg aattcaggcc
     4501 ggcaatcgat ggcagtttgt tgcgccggcc ctgagctgcc tatggagaca agagaagctg
     4561 ggctcactgt ggggatgccg gtctgcaacg gaatgccgtg gctgccggaa gcgatggaca
     4621 gtctgctgca gcagacgacg aaggcctttc agatccttgt gatcgtggat ggcggatcgg
     4681 atgatagtgc gacatggctg agaaatcttg agaacaggct ggcacgcgtg gggttcccgt
     4741 ggccacgcct gcgggttctg gagcaggagc atgcaggaat aacagcgact ctgaatcggc
     4801 tgctgtacga aacgcgcacg ccctggctgg tgcgcatgga tgccgacgac gtttcttatc
     4861 ccacgcgcct cgaaagactg cagattgcaa tcgaggaaca ttccgatgcg gggttgattt
     4921 actcgctggc ggactattat ccgcaggacc ggtgcgcggg aaggttccgt tgctcgcggg
     4981 gaacaccgga ggagctgcag gctatggttc gcagcggcta tctgctgtcg atctgccact
     5041 cctcggtagc gctcaacgtg gagaaggttc gatcggttgg aggatatcgg atgaatctcc
     5101 atgcagagga tgccgactta tggtggcgtc tggcgctgcg atacaagatc catttcattc
     5161 cggagccact ggtggggttc cgcattcatc cgcgaagtgt cagctcaagc aatcttgaga
     5221 cgcagcagct gggcggtgtg tacgtgcagt atttgctgct gtccgagttg tggggtaaga
     5281 agccacgggc gttcggagag atcgcagagg ctttgggcga gtttgttcag cccgcggcat
     5341 tcgaggccaa ggaagcgctg cgcaaattca atacactgct ggcggagaag cgctatgtgc
     5401 gcggacttgg agcgctggct cacgcatttc atgcatctcc tggatatgtc tcgcggcgag
     5461 tgcgggaaga attccgtccg gcagccatcg gcaacggcgt tgcgcccgcg aaattctggc
     5521 agcggaagga agagctgtgg gcgtgatcgg ggatctttca gctcctggca cagacgctgg
     5581 aacggagacg gcagcccgtc tgccgtttcc gccgcgattc ctgctgggca ggattccggt
     5641 cgatcggatc acgatggact acgccgccgt gctgctggtc gaagccctgc tgcatcgggg
     5701 cgccttgccg ccgctgaccg tggttgggcc gaacgctcac ctggtaacga tggcggagcg
     5761 cgatagccgt tttgcggaag ccatgtgcga tgcggatttg gcggtgcccg acggggtttc
     5821 cgtcgtgatg gcatcgcggt tcctgggttt cccaattccg gagcgagtga ccggtggcga
     5881 cctcatggag cggatgtgcg cggaagcggc gcactatggc tttcgggtgt tttttctggg
     5941 cggtcttctc ggcgcggcgg cgatggccgc acaccacttg tgcagccggt acccggggct
     6001 tcagatttgc gggacctatt gtcctccggt gggattcgag catgatccat ccgagatgga
     6061 aaggatgcgc agagcgattg ccgatgcgac accggatctg atctgcgtgg cctttggagc
     6121 gccgaaacag gagatctgga tgcagcagaa ccgggagcag ttccacgtgg gcgccatgat
     6181 ggcagtgggt gcggctctgg atacccaggc aggactgcgg cgaagagcgc cccgctggat
     6241 tcaggccatt gcgctggagt ggttgttcag gctggcgatg gagccgcggc ggttatggcg
     6301 gaggtatctg atcggcaacg cggaattcgt tctgctcgtg cttgggcagt ggatgcggga
     6361 gcgatgggca ggattacgcc gatggctcgc gccggcgatt gccatccgcg aaacagaaaa
     6421 tccgggatcg acaggaatgg gccatgaaga agattgagga cttacgcatt cggctgttca
     6481 gtgacggagc agacttcgcc ggaattatgg agatgtattc acggccctgg gttcgtggat
     6541 ttacaacgaa tccgacgctg atgcgcaagg ctggcgtgcg gaactacgaa gcgtttgcgc
     6601 gctcgctgct gcgcgcgata ccggatcggc cggtgtccct ggaagtgttt gccgatgacg
     6661 aagagcagat ggagatgcag gcgctgacgg tggcgacctg gggtgcaaac gtcaacgtga
     6721 agatccccgt cactaaccgc gccggtgtgc ttcagacccg gctgattcgc actctcagcg
     6781 aggcgggagt ggccgtgaac gtgaccgcca ttctgaccct ggaacaggtc gaacaggtgg
     6841 ttggcgctct ggcggcggag acgggggcga tcgtgtcgat tttcgccggc cgcattgcgg
     6901 ataccggcgt tgatcctgtt ccggtcatgg cggaagcgaa gcggatcctg cgctggcggc
     6961 ctcgcgcgca attgttgtgg gcaagcccgc gagaggtcct gaacgtgttt caggccgagg
     7021 atgcgggctg cgacatcatc actctgccgc cggagatgct gcgcaaactg gaactcgtgg
     7081 gccgggatct aaagcagtac tcgctggaga ccgtggagat gtttcatcac gacgcgcggg
     7141 ctgtgggtta cgtgatcgat accagcggtc tggcagctgc gaaggccagc ggacaacctg
     7201 ccggcagaac ggtcgagaat aatttcggcc gcttccgcga gatcacgtgc gatgtagtcg
     7261 gcgcagggat cgggtccgca tcgaccggcg tcgaagcgtg attccggttc gatcacgcgg
     7321 attgtccggg tacccgcagc gcggccgcac tgcgtgtccg ttggctggtc tccgatcatc
     7381 caggactcgg ccagggtgag gctgaaatcc tcagctgcct gcaggagaag tcccggagaa
     7441 ggtttgcggc attcacagat ttgcgaatag cctggcgtga ggcctttggg gtgatggagg
     7501 cagtagccga agcgcaggaa ctgtatccct gcggcggcca tgcgggtgac gaactcctgg
     7561 tggatgcttc ggaacgcttc gtagctggac ttaccaagag cgaagttggg ctgattcgac
     7621 acaaggatga ggggatagcc tgcgtcctgt aggtgcagca gagcgggaat caccccatcg
     7681 atcaggcgaa agtcgctgga cgcaagcggt gcttccagtc ggcgggtcgc agggttgagc
     7741 accatggcgt tgagaacgcc gtcacggtca agaaaaacgg cggggcgaat catcgcgcgg
     7801 attcccactt cattgggttg gctcgcagcc gcgggtggga aacgatcagg tgccagataa
     7861 ccgcctggaa ctcctcggtg tggggagtga ccgtttccgg cgataacgtg ggaacgatca
     7921 ggcacgcatc ggcgagctga gccgtgtaac cgccatcgcg tccgaccaca cccaggatgc
     7981 gtgcgcctgc ctcgcgggcg agattgagac tggcgacgag attcgtgctg atgtggcgct
     8041 cgacgctgcc cccgccgaca gagaagatga agacggcatc gcgagaggag agacggctgc
     8101 cgcggagcca gttggcgtag caggtttccc atccgtcatc gttgatgcgg gcggtcagct
     8161 ccgagacgtt gtccgtcggg gtatagcatt ccatgccggc gatcttgcgg aagtcattga
     8221 ctgcatgtcc ggcgtgcccg gccccgcctc cgaccccaag gaagaagagg cggcctccct
     8281 gggcgcggac ctcgtgaagg agatcgatca tgcgatcgat gacggtcgta tccaggtggg
     8341 cggcgagcgc ggcaacttcg gacaggtaga cggaggtgta atccataaga ggcacggttg
     8401 agacgatcag gatgaggagc gccgcaggat cggtgtcgag gttttacggc tgaacacgag
     8461 acgcaaaccg ggcgcagggt ccgaagccgc ccgtttcctg agaaagcccg cacgaaagaa
     8521 acggcgaacg cgaactggca cgaatgcaaa gatcagaaaa ctgacgagcg aacggaattc
     8581 gggagcgcct tcctggagaa aagcatgcag caggcgaaat ttcgcaagcc acccggcacg
     8641 acttgcctgg gccctccacc cgcactgaat ggcgacaaac caggaaaacg cccgtggaga
     8701 aatcagggag cgcacgctgc gaatccagga aagagaattt ttccaggcgg gatctctgcc
     8761 gacggttgac cggctgtcct cgacacgcca aatggcgaga ggctggcgaa ccatggacag
     8821 gcccacgcct ttgtaagacg cgacccggat aagccagtcc cagtcctgat gcatgggcag
     8881 accggcccga aatggaaccg cgattagcag gtcgcgcggc gccagcaggg tggaggtctg
     8941 catcaccccc ccagggtccc gcagcgaggc acggcagaat aagaaatcgg cgatcgccct
     9001 cggggcttcg taaagctgct cgggcagaac gcggctgacc gagggggatt gcgcgatcag
     9061 gcggcaggac acgaccggaa accagtcggg cattgcatgc gccgcgtgca tctggcgctc
     9121 aattttctcc ggtagccatt cgtcgtcgtc gtccaggaaa gcaatccagt cgccgcgagc
     9181 cacacgaacg cccacgttgc gagcctcgga gccgccgcag ggttccggca agacgacgac
     9241 gcgcacgcga ggatccgtga tggctttcag ggagagaacg gttgcggggt ccgggccatc
     9301 gaccacgacg atgacttcga tcggagacca ggtctggcgc agcgcgctgc gaacggcaca
     9361 tgcaagcagc cgggggcggc catgcgtggg aattacagca gaaacaatcg ggtaactcgc
     9421 acctggaaag ctatcagaca tcagaaccac acgatagaaa gtcggatcgt tgttctgaga
     9481 gtagttctgt cagcacgacg acaaaagatt acgggcgagt tcggattcac cgaagtaacg
     9541 ccacgtgaag atggttggat gtgataggac cgcgccggag aatccgggca gacagcatga
     9601 aagtccgggg gccgcacgcc gaagagaagg gtgcggcccg cacgatgtca gcggttcatg
     9661 ttatcgaata ccgaaccgac gatgccgccg agcaaaccgg cctccgtgct ttgccgcacc
     9721 tcgcgtcccg cgggcatata ttcctgcagt tgatgagcga ggcgggaaat gggcagcgtc
     9781 tgcagccaga tgcgacccgg cccggtcatg gaggcgagga agaggccgtc tccgccgaag
     9841 atcaagtttt ttatgccgcg gaccatggtg atctggaacg agacgctcgc agtgaaagcg
     9901 cccacatgtc caggatgcac gagcagagtt tctccctgct ggagatcgcg ggtgacaacc
     9961 tcgccggaga gttcaagcca ggccagtccc tggccgctga tcttctgcag caggaagccg
    10021 tcgccgccga agatgccggc gccgagcgac tgctggaagc cgacgccgat ctgaacaccg
    10081 gcggtggcgc agagaaaacc atggcgatga accatgtagt cgtacccggg cacgacttca
    10141 accggaacga tgtgacccgg tacccgtgtg gcaaaggcca gttcagcatt gccgcccgca
    10201 gcgcggtatt cggtcatgaa caggctgccg ccgccggcaa cacgcttgat ggcgccgaag
    10261 atgccgccgc ccccggcgaa ttgggtatgg gttgtcatct gaacgttggc agacatccag
    10321 gacaactcac ctgcttcgga gatgatggcg tcattcggtt cgagcaggaa ctcgagaacc
    10381 ggcatcgtcg tgccaatgac ctgactcttc atgcggaaca gggtacagga ttcggctgcg
    10441 aatcgtcatc cgcgatcgcg tgaaacgcag cgggaaacgg gcgggcggac cttgcggtga
    10501 agacgctcaa ggatgaaaaa agtgcagcga atttcccgga aaaagagaac aaattgcacg
    10561 aatttttgct gtgcagacga ggaggcagag aacagcagaa aaatcctgag aaatttgaat
    10621 ttttggggtt gcacgaagcg ttcacgagga gtaccagtag ggtaatagtt cctttagaac
    10681 tgccacggag gatcgagatt tactgatcga ctttgtgagg taggggccag aagtgaccag
    10741 cttgtacgcc acgctgtcag ttgttaacac cccgttcgat tgattcgctc ttgagcagta
    10801 gacgcgatca gagtcgcagg gttcccgccg aaaattagac ccgcctggta tctcagtctg
    10861 agagagaaag gtattgtctc gcttcgacga gccatggaag ccgccgtgtg tgcggcgatc
    10921 atgccgatcg aagtggacag cgcctgacac tcagctgcgc ctttccggtt gtggaaaggc
    10981 acggcgccgc agcagcgctg ccggcgtcaa cggctgaagt gtgcccagac gcgatgaggt
    11041 agatggaatg aatttgaata ttttcccctc gatgttcggc cgatcggtta cggccgtcga
    11101 tgcagcgtgc tgcacccacc cttcactcgg gaagacactt cctgcgcgca agcgcctggc
    11161 aaatcgttac agcaaagttg tgttcgcggg cgttgcattg gcaattctgt gctgcactcc
    11221 cggcagcctg tccgcacaga atcaccgctg gcatcgctgg ggtcgctggg gaggatcggg
    11281 t
//
LOCUS       DASZYN010000126         3982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_69805, whole genome shotgun sequence.
ACCESSION   DASZYN010000126 DASZYN010000000
VERSION     DASZYN010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69805"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VHE33_03265"
     CDS             <1..438
                     /locus_tag="VHE33_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96497.1"
                     /translation="AEPEAEQQTGSFAKGLEIWMSDGERAVCVLADADEIPGALAMFD
                     AWLRLARFPPAFQRLDQRLVGLHYRFREGLTIGVGGEPAESSPEPDTFFDSVAPIPEI
                     DDFREPHFGVRLPESAVGYLHDLLRSAAAWLEDNSYPNILGPR"
     gene            435..1052
                     /locus_tag="VHE33_03270"
     CDS             435..1052
                     /locus_tag="VHE33_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96498.1"
                     /translation="MKPGFSAGMLAALLTLSLSAAAQDSPQAPTTATNAERTETGSML
                     SAEGQSIEYRIRLLPVSSFPDLPAAVAAELNRRGCMIPQSFEAKQPENVIHGAFRAAG
                     SSDWAALCSVHGTTTLYVFFAGADAPIALRSQPDAAWLAAEPGNPVPGSAWGIANRPV
                     EELRSTPALRRKITIDHDAIDDARLERSQVLHYYSAGQWVILDGQ"
     gene            1308..>3982
                     /locus_tag="VHE33_03275"
     CDS             1308..>3982
                     /locus_tag="VHE33_03275"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HVT96499.1"
                     /translation="MMDRIDPRGIPQFLAADRTRHSIGFLALQRMAMSPSLLRTIRPI
                     AIFASLASGAVLGAPLLAQSAQFTVPSRLVRPIDDNSRITLHGYVHPLANAANDRGAA
                     PDSMPLERMHLVLKRSDAQETELKQYIAGQHTPGSASYHKWLTPDQFGKQFGPSDQDV
                     ATVESWLSAQGFDVVGLKPGRQVIEFTGSVAQLRTAFHTQIRQYEANGNLHFAAANEP
                     QIPAALSPVVGGFVSLNNFRPQSHARMLGRAFYDPKTGTAKPDWTVNGGEGYPTIGGV
                     NFAVTPGDFGVQYDLPNASLNPAYSGTTYDGTGQTIAIINEANINIALVNQFRTLFGL
                     PANPPNIVIDGNDPGIDGINNPDGPNYATSEAYLDVEWAGAVAPKATVDLVIAADTAL
                     ENGLYLAAEHAIYSNLAPVVSVSFGACESAIGSTNAFLLALTEQAAAEGITVTVSTGD
                     NGSAGCDNPNAVQYASYGAAVSGFASTPYDVAVGGTDFYYSNYQHLALTDLSTYWNTT
                     GSNTPSVTLKTPIPEQPWNNSQFGLDASNYYSTYQATTIGAGSGGASSAAVCAAGYNQ
                     TTGVCLGTPTGYPKPSWQMGTGVPADNVRDIPDVSLFAADGPNYAYYATCVSDGDCQP
                     NSSGPVQIFGVGGTSASAPAFAGIMALVNQKYGRQGQADFVLYPLKAQFPAAFHDVTV
                     GSISVPCKIGTVNCISVASPITIGGVSEGQIGSGTTPYYNASAGYNLATGLGSVDATV
                     LLNNWGNISFTGSTTTLTPSSTSFAHGTAITISGSVTGTGSPTGNVALLADTTLPVQQ
                     AQATFSLSAGAFSASNVNYLPGGTYNIWGQYGGDGKNAASTSAKTQITVTPEASTTTF
                     DVLNSASTSSASVITAGQSVSYGTQLILAAQVHGT"
BASE COUNT          729 a         1293 c         1223 g          737 t
ORIGIN      
        1 gccgagcctg aggccgagca gcagacgggc agcttcgcca aggggctgga gatctggatg
       61 agtgacggcg agcgcgcggt gtgtgtgctg gccgatgcag acgagattcc gggcgcgctg
      121 gcgatgttcg atgcgtggct gcggctggcg cgtttccctc cggcgttcca gcgcctggac
      181 cagcgcctgg tgggactcca ttacaggttt cgcgaaggac tcacgatcgg cgtgggaggc
      241 gaacccgccg agagctcacc cgagccagac acgttcttcg acagcgttgc gcccatcccg
      301 gagatcgacg attttcgcga accgcacttc ggcgttcgat tgccggaatc ggcggtgggg
      361 tatctgcacg acctgctccg ttcggccgct gcgtggctgg aagacaattc gtatcccaac
      421 attctcgggc cgcgatgaaa ccagggttca gtgcggggat gcttgccgcg ctgctgactc
      481 tctccctttc cgccgccgcc caggattccc cgcaggcccc aaccacggcc accaatgcgg
      541 aacgcaccga aaccggttcg atgctttcgg ctgagggcca atcgatcgag taccgcattc
      601 gcctcctgcc ggtttcttcg tttcccgacc tgccggcggc ggtggcggcc gaactgaatc
      661 ggcgcggatg catgatcccg cagagtttcg aggcgaagca gccggagaac gtgattcacg
      721 gcgcctttcg cgcggcgggc agcagcgact gggcggcgct gtgctctgta catggaacca
      781 cgactctcta tgtgttcttc gcgggtgcag acgcgccgat cgccctgcgt tcgcagccgg
      841 atgcagcgtg gctggcggca gagcccggca accccgttcc cggatccgcc tggggcatcg
      901 cgaatcggcc ggtcgaagaa ctgcgctcga caccggcttt acgccgaaag atcaccattg
      961 atcacgacgc gatcgacgac gccaggctgg agcgttccca ggtccttcac tactactcgg
     1021 cgggccagtg ggtgattctc gacggccagt gacagcagtg actcggccca tcgtggcttc
     1081 gaagttccgc tctgttattt cttctctcga ccgcaactaa taccggcgtc agttgtctaa
     1141 agcatcgcag cgcctgtggt tcgtttctat ttttctggct cccgagcttc cccgctttcg
     1201 ggcctgttgc tgtgcgtccc gaggacgtcc gcgccgccgg cctgagtccg atcgttcaac
     1261 gaaaatccgc cccatttaca ggacgccgcg ttcaccaggg cagggttatg atggatcgca
     1321 ttgatccgcg cggtatcccg cagtttctcg ccgcagatcg cacgcggcac agcattggtt
     1381 ttctcgcctt acaaaggatg gccatgtctc cgtcgctcct ccgcacgatt cgccccatcg
     1441 caattttcgc ctccctggct tccggcgccg tgctcggcgc cccgctcctg gcacaaagcg
     1501 cccagttcac cgtgcccagc cgcctcgtgc ggccgatcga cgataattcc cgcatcactc
     1561 tgcacggtta tgtacatccg ctggcgaatg cggcgaacga tcgcggcgca gcgccggact
     1621 cgatgccgct ggagcggatg catctggtcc tgaagcgcag tgacgcgcag gaaacggagt
     1681 tgaagcaata catcgcgggc cagcacacgc cggggagcgc gagttatcac aagtggctta
     1741 cgccggatca gtttgggaag cagttcggtc cctcggatca ggacgtggcc acggtagagt
     1801 cgtggctgtc ggcgcagggg tttgatgtcg tcggcctcaa gccgggccgc caggtcatcg
     1861 agttcacagg cagcgtggcc cagttgcgca cagccttcca tacgcagatc cggcaatacg
     1921 aggccaatgg caacctgcac ttcgccgccg ccaacgagcc gcaaattccc gcggcgcttt
     1981 cgccggtggt cggaggcttt gtctcgctga ataatttccg gccgcagagc cacgcgcgta
     2041 tgctcggccg cgccttctac gatccgaaga ccggcacggc gaaaccggac tggacggtga
     2101 atggcggcga agggtatccc accatcggcg gcgtgaactt cgctgtgacg cccggagatt
     2161 tcggcgtcca gtatgacctg ccgaatgcct cgctgaaccc tgcgtacagc ggaaccacct
     2221 acgacggcac cggacagacc atcgcgatca tcaacgaggc aaacatcaat attgctctcg
     2281 tgaatcagtt ccgcacgctg ttcggattgc ctgcgaatcc gcccaacatc gtcatcgacg
     2341 gcaatgatcc ggggatcgac ggcatcaaca atcctgacgg ccccaactac gcgaccagcg
     2401 aagcctatct cgatgtggag tgggccggcg cggtggctcc gaaggcgacc gtggatctgg
     2461 tgatcgcagc cgataccgcg ctggagaatg gcctttatct ggccgccgag cacgcgatct
     2521 acagtaatct cgcgccggtc gtcagcgtca gctttggagc gtgtgagagc gcgatcggct
     2581 cgacgaatgc gttcctgctg gccctgaccg aacaggccgc tgctgaggga atcacggtca
     2641 cggtttcaac cggcgataac ggatcggcgg gctgcgacaa tccgaacgcg gtgcagtatg
     2701 ccagttatgg ggccgcggta agcgggtttg cctcgactcc ctacgacgtg gcggtcggtg
     2761 gcaccgactt ctactacagc aactatcagc acctcgccct gactgactta tcgacttact
     2821 ggaataccac cggcagcaac acgccgagcg tgacactgaa gacgccgatt cccgagcagc
     2881 cgtggaacaa cagtcagttc gggctggacg cttcgaatta ctacagcaca taccaggcga
     2941 ccaccattgg cgcgggcagc ggcggcgcca gcagcgcggc ggtgtgtgcc gccggctaca
     3001 accagaccac gggagtctgc ctcggcacgc ccaccggcta tcccaagccc tcctggcaaa
     3061 tgggcacggg cgttccggcc gacaacgtgc gtgacatacc tgacgtttcg ctgtttgccg
     3121 ccgatggtcc gaactacgcc tactatgcca cctgcgtctc ggatggagac tgccaaccca
     3181 actcgagcgg gccggtgcag atcttcggcg tgggcggcac atcggcttct gcaccggcgt
     3241 ttgccggcat catggcgctg gtcaaccaga aatatggacg ccagggccag gcggactttg
     3301 tgctgtatcc gctgaaggcg cagttccccg ccgccttcca cgacgttacc gtcggatcga
     3361 tttcggttcc ctgcaaaatc ggcactgtga actgcatctc tgtggccagc ccgatcacca
     3421 tcgggggagt atcggaaggc cagatcggtt ccggcacgac accctattac aacgccagcg
     3481 cgggttataa cctggccacc ggccttggct cggtggatgc gacggtgctg ttgaacaact
     3541 ggggcaacat cagcttcaca ggaagcacca cgacgctgac tccgtcgtcg acaagctttg
     3601 cgcatggcac ggcgatcacc atcagcgggt cggtgaccgg caccggcagc ccgactggca
     3661 atgtggcgct gctggccgac acgacgttgc ccgtgcagca ggcgcaggca acgttctcgc
     3721 tgagcgccgg tgccttctcg gccagcaatg tgaactatct gcccggtggg acctacaaca
     3781 tctgggggca gtacggcggc gacggcaaga acgcggccag cacctcggcg aagacgcaga
     3841 tcacggtaac gcccgaggcc agcaccacca cctttgacgt gctcaactcg gcgtctacat
     3901 ccagcgccag tgtgatcacc gcgggtcaaa gcgtctctta cggcacgcag ctgattcttg
     3961 ccgcgcaggt ccatggcacc ag
//
LOCUS       DASZYN010000127         1892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_966705, whole genome shotgun sequence.
ACCESSION   DASZYN010000127 DASZYN010000000
VERSION     DASZYN010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966705"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VHE33_03280"
     CDS             complement(<1..206)
                     /locus_tag="VHE33_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96500.1"
                     /translation="MTRTLKALATLALTAAVATGFAQTTASSPGTKKVHRKVAPKGPT
                     VQQQIEELRQQQQADHGQIDNLKQ"
     gene            complement(524..>1892)
                     /locus_tag="VHE33_03285"
     CDS             complement(524..>1892)
                     /locus_tag="VHE33_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96501.1"
                     /translation="QQGLQLARTWLDQPDNSKWQITRIGAPGLDTVSSNSGDAIFRVY
                     VPREAAVTRPYFTRPNTEQPYYDITDPHWLPEPFAPYSLAGWAEFHYMGVPIRVGEVV
                     QTVHRVHGIGGVYQPLAVVPQISVNLPASAGVVPLGTATIPFSVTVQNEQQSDADGTL
                     QLKLPAGWTAEPPQAPFHLGPGAGQPMQFTLHPTELGDQTYEIGAVAQTGNFSFREGL
                     TTVGYPGLRPYYLYRPATYRLRAVDVKVPASLRVGYIMGTGDDVPDALREMGIEPHLL
                     TPIDLAQGDLSAYDTILIGIRAYSSRPDLEAATHRLFDYVRSGGNLIVQYQSNQFPAP
                     YTLALGRNPEKVVDENAPVTLLDPNAAIFSSPNHITSADFDGWIEERGHSFLASWSAE
                     YTPLTEVHDPGQDPQKGGLLETHYGKGTYFYLAYAVYRQLPEAVPGAYRLLANLVAAG
                     KHPQP"
BASE COUNT          379 a          467 c          680 g          366 t
ORIGIN      
        1 tgttgcttca ggttgtcgat ctggccgtgg tcggcctgct gctgctgccg caactcttca
       61 atctgttgct gcaccgtcgg tcctttgggt gcaaccttgc ggtgaacttt tttcgtgccg
      121 ggggagctgg ccgtggtctg cgcgaaaccg gtggcaacag ccgcggtgag cgcgagggtg
      181 gcgagcgcct ttaatgtcct ggtcattcag cctctcaaga gttggaagtg cgtggagatg
      241 aagccgatcg gggaacgtgc cgggctccag gagaacgcgc cggggaacga cgtgccgatg
      301 tgtcacgcgc cgactcgccc cttggttacc catcggcatc gtcgcaggcg attgtgaatg
      361 ccgggagaat atgcggtaaa tgttcgagga aaaggccgcg aaaggatgtg ggcaagttct
      421 taaagggtca acagcttaca acgagcacaa cgccagccac atcctcgcag tggaaatgcg
      481 gagacatggg cgagacgagt gttttcggcc gcagtgggag tctttagggc tggggatgtt
      541 ttcctgcagc aaccagattg gccagtagcc ggtacgctcc ggggacagcc tcaggaagct
      601 ggcggtacac agcgtaagcc aaatagaagt acgttccctt gccgtagtgg gtttcgagca
      661 agccaccttt ttgcgggtcc tgtccgggat catgaacttc agtgaggggg gtgtattcag
      721 cggaccagga ggcgaggaag gagtgaccgc gctcttcgat ccaaccgtca aaatcagcgg
      781 acgtgatgtg gttgggcgag gagaagatgg cggcgttcgg atcgaggaga gtgacggggg
      841 cattctcgtc gacgactttc tccgggttgc gtcccagagc cagcgtatag ggagcaggga
      901 actgattgct ctggtactga acgatgagat tgccgccgct gcggacgtag tcgaagaggc
      961 ggtgcgtcgc tgcctcgaga tcaggccgcg aggaataggc acgaatgccg atgaggattg
     1021 tgtcgtaggc agagaggtcg ccctgggcga gatctattgg agtcaggaga tgcggttcga
     1081 tgcccatttc gcggagggca tcgggaacat cgtcaccggt acccatgatg tagccgaccc
     1141 ggagactggc aggcaccttc acgtcgacgg cgcgaaggcg gtaggtggcc ggacgataca
     1201 gataataagg acgcagtccg ggatagccaa cggtggtaag accttctcgg aagctgaaat
     1261 ttccagtttg agcgacggca ccgatttcgt aagtctgatc gccgagttcg gtggggtgga
     1321 gtgtgaactg catgggttgg cctgcgccag ggccgagatg gaacggcgcc tgggggggct
     1381 ccgcggtcca gcccgcaggc agtttcaact gcagggttcc gtcggcgtcg ctttgctgtt
     1441 cgttctggac agtgacggag aagggaatgg tggcggtgcc aagcgggacg acgccggcgg
     1501 atgcgggaag attcacggaa atctgcggga cgacagcaag gggctgatag acaccaccga
     1561 tgccatgcac gcgatggacg gtttgaacga cttcaccgac acggatggga acgcccatgt
     1621 aatggaactc agcccaaccg gcgagggaat acggcgcaaa gggctcgggg agccaatggg
     1681 gatcggtgat gtcgtagtac ggttgctcgg tattggggcg ggtgaagtac gggcgggtga
     1741 cggccgcctc gcgcgggacg tagacgcgaa agatggcgtc gccggagttg ctgctgacgg
     1801 tgtcgagtcc gggggcaccg atgcgcgtaa tttgccactt gctgttgtcg ggctgatcga
     1861 gccaggtgcg cgccaactgc aagccttgct ga
//
LOCUS       DASZYN010000128        25942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1226388, whole genome shotgun sequence.
ACCESSION   DASZYN010000128 DASZYN010000000
VERSION     DASZYN010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 25942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25942
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226388"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VHE33_03290"
     CDS             <1..530
                     /locus_tag="VHE33_03290"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HVT96502.1"
                     /translation="AQMFAQREMRKTYLALVHGHLKQDRGTINAPIARDLLRRTRMTT
                     RRQAGARTAISHYTVLRRIASRFGPLTLVSVRIETGRTHQIRVHLASIGHPVVGDTLY
                     GAPARLMVIPGRRTADPEPESLALKRNFLHAAELEFAHPRTGKPMALQSPLPAELEEL
                     LSQIESSGAAKPLTP"
     gene            564..893
                     /locus_tag="VHE33_03295"
     CDS             564..893
                     /locus_tag="VHE33_03295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVT96503.1"
                     /translation="MAKPSDLVQGTLDLLIMRTISPEPMHGWAIAKRIQQISNEVLQV
                     QQGSLYPALHRLEQQGWIRAKWMESETGRQAKFYSLTAAGKKQLEEERANWSRLSAAI
                     DLVIENA"
     gene            956..3604
                     /locus_tag="VHE33_03300"
     CDS             956..3604
                     /locus_tag="VHE33_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT96504.1"
                     /translation="MRILEKLRMRVQMLLHRERESDRLNAELEFHLEQQIAENRAAGM
                     SPDEARAAALRSFGNPVVLREQARESWSWNWAEALSRDIRIATRTLLRSPGFAMTAIV
                     VMALGIGANVALFTLVRSVLLRPLPFAAPGQLVGIFEAKADGSFQDNIVAGGCFGRWK
                     EQSHSFAALALSQSTSYNLAGTGNQLPEVVEARTASWNMFSLLGVQPALGRFFTETED
                     QRGANATAVLTWGLWKRRFGGDAGIVGRTILVDAKPYTVLGILPAWFTWPDPRVQLWT
                     PLYHEKSPEMMRMFDAHSFDAVGRLRPGVTMQQASAELNGIQRQIRREHPDGPVNDAV
                     NLRSLIDADTYDIKGGLYALLGATGCLLLIACLNIANLLVARSAARSKETAIRTALGG
                     SRLRLLREQVVESMLLSLAGGLMGLLLASGILHWLISTREDIPRADSIHMDGVVALFT
                     LAAVLGCGILAGLIPALGARDAHVLTTLQELSRSYSGGRSRARVRQFLLALEVALTVV
                     LLIGAGLLLRSYQQLRSADLGFAQHNILTMRITLSRNSYPTNASRVAFFDQLLSRVRA
                     LPGAQAVGLTSALPGEGRQRNDVFVIAEHPPLPRGQVIDALTRFVDPGYFKAIQIPLI
                     EGRTLEPSERLDQSQVVVVNQELVKEYFPNEDPIGRHVKAEMVLGTTPLRIVGVVGNT
                     RDAISHDPFPVIYYPLFSGDQRGVSLVVHSRRDPAGIALSVQKAVASIDPQLPVSDVL
                     TMEEVLGGSTLDARFDAILLVAFAILSMLLAMVGLFGVLSFMVAQRTTEIGVRIALGA
                     SRENIMRKMLRDGLRPAIYGLVLGLAASMGAARLIQSLLFGTKPFDPAVFVLVSFTLL
                     LVAALACALPAWRASRLDPIQALRAE"
     gene            3670..4101
                     /locus_tag="VHE33_03305"
     CDS             3670..4101
                     /locus_tag="VHE33_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HVT96505.1"
                     /translation="MQVLYTADVTAEGARHGHVRSSDGLLDFDLALPPGMGGKGDKTN
                     PEQLFGAGYAACFGGAVEYQAMLAKKKIGVVTVRAHVSVGPFDEGQGEGFTLRVELDV
                     TIPEVDQATAEELVAAAHRGCPYSNATRGNIEVKLTAHGGR"
     gene            4194..5111
                     /locus_tag="VHE33_03310"
     CDS             4194..5111
                     /locus_tag="VHE33_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVT96506.1"
                     /translation="MQSERRATAFLALAGGVISISWSAIFVRWARMPGTCSAFYRTLI
                     ASVVLWGMFPFLRDKSRRLSRRSFWITVLGGALFAGDVGLWNTAVMHTSAGNATFLSN
                     IAPLFVGLFTWIFTRHLPSRRFWIALLVSTLGSGLIVAADLRLALGRSSADGLALLAA
                     VCFALYLIITSRLREKHDVLELLALSTTASAVVLLIAAGAARLPMNIPSAGSLWALLA
                     LGLICQVGGYFGLTYALGHLPATVTSILFLSVAPMTAIFAWIIFGERMSLVEIAGGAL
                     ILSGAWIVGRKEKPEVPQVEELAPPVAEA"
     gene            complement(5116..7362)
                     /locus_tag="VHE33_03315"
     CDS             complement(5116..7362)
                     /locus_tag="VHE33_03315"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HVT96507.1"
                     /translation="MASVQPSALQVERVPDLFREKFGKLLDTVHKNRPSDDVNIIRQA
                     WQFCLEHHNGQLRASGEPYVLHPLEVALVLAEMKLDSTAIAAGLLHDAVEDTPVTTED
                     IVARFGEQVAHIVEGVTKIDKIQFANREDRQAENVRKMLLAMVSDVRVVLIKLADRLH
                     NMRTLEHLAPERQRAIARETLDIYAPLAHRLGMGKLRGELEDLAFRWVDPITWEQLHE
                     AIEARRSEGEQFLAGVEGLLSERLHENGIKAHVEWRIKRLYSIHSKLVKSQTSIDQVY
                     DLLALRVITTSVQDCYAVFGIIHSIWRPVPGRIKDFIAMPRPNLYQSLHTTVMGEGGH
                     QFEVQIRTEEMHRIAEEGIAAHWKYKAGGSPVSARDEQRLAWVRQLVEWQRDMTDPNE
                     FLSTLKIDLYPEEVYTFTPKGKVVVVPRDGTPVDFAYTIHTEVGNTCVGARVNGRMVP
                     LRTKLRNGDIVEVITQNGHTPSRDWLTFVKSPRARNKIRHWLNEHQRERAIEIGRKLL
                     EREARKYKVGLSQFEESDYARVAADYSVATPNDLWAAIGFGKYSARQVLNRLAPGLIH
                     QQQESEAAATPASPGADAVRRAAAASGTDSLIVEGQNELLVYRARCCNPIRGEEIIGY
                     VTRGKGVAVHARTCPNVQNLLYEADRRIAVEWARSPEDKAGHKQTYPVRFTVHCDDRA
                     GMLKEITAVISDENANIRTMETHLGDAGDAQIQFVIDAEDVKHLTKLVQALRRLPGVR
                     DVQRSQKL"
     gene            complement(7429..8811)
                     /locus_tag="VHE33_03320"
     CDS             complement(7429..8811)
                     /locus_tag="VHE33_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVT96508.1"
                     /translation="MKGFLPRFALVLMLTVLAAGALAQTSGENPAGVSSQSAGSQQGA
                     ASASAPAPQPGQGAPNSRSSPSAPATATEKSAPGPAPVSANTTSAPSGPPAVPVTTEI
                     GKINGASFRIDVPKDWNHGLVLYFHGYQQTPQVFDPKYPPTRVLQEILNRGFAVARSG
                     YAVGGWAVPQAAAETEALRHYFRRKYGQPRETLLMGHSMGGLLTLMGLEQNPKEYAGG
                     LALCGVLAPADLVMQRAFANRVAFDAFFPGALPDVEHIPPTFAMNDIPTITKIQKSLD
                     ANPDQAALVRQIANIHTNRGLADVLVFNTFVIEDLRLKSGGNPFDNRNLVYSGTVNDA
                     LLNQRVHRYASDPNAYRFLADWYSPTGNLHRKLLEVHTIYDPLVPSSTTAWYDELTRR
                     VGNGGNFVQQYVDRDGHCNVTADQTGLAFDELLAWVHENRRPTPGLLPGSPPPPVKTP
                     PKPKNPGKPE"
     gene            8959..10275
                     /locus_tag="VHE33_03325"
     CDS             8959..10275
                     /locus_tag="VHE33_03325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HVT96509.1"
                     /translation="MASGKQAEANHGTHSRQDAIQTREFQPAVPGAIGISRRAADRLR
                     AGHVWVYRSDIEAWPATPDAIPAGLVPVTDRRGALLGTGLYSPASEIALRLVSTDLIQ
                     TEAEWLDLLRSRLQAAVARRGPLLDAENHACRLVFSEADELPGVTVDRYAGLMIVQLR
                     HKALDTDATRAVVTETLRNALDPETGELETILEREDPRIRELEQLSAPASSTLFARDP
                     EHPATRTVFRLNGLQFHFDATSGQKTGAFLDQRENYAAAARYAAGEALDVCTYQGGFA
                     LHLARVCPRVTGIDVSRAALEVAEQNLALNRDLLGNCAVDWVEADVFTLLRDWSDAGS
                     VYDTIVLDPPAFAKSKRAVEGALRGYRELNLRALKMLRPGGRLITCSCSHHVSLADLM
                     ETLRAAAGDARRRVRLLEVRGASPDHPVILNIPETEYLKCVILEAE"
     gene            complement(10304..11491)
                     /locus_tag="VHE33_03330"
     CDS             complement(10304..11491)
                     /locus_tag="VHE33_03330"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF010611.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HVT96510.1"
                     /translation="MKLPIYMDNHATTRIDPRVLDAMMPYLTEAYGNAASRSHGFGWA
                     AEQAVETARARVAKLIGATAREIVFTSGATESNNLAITGAVEAQGSRGSVITQATEHK
                     AVLDPCVWIEKRGCRVTVLPVDGEGRVRIADLQRALNEAGGEEKPLLVTILAANNEIG
                     TLQPVNEIGALCHERGVLFHTDAAQAVGKIPIDVNRSHIDLLSISGHKIYGPKGIGAL
                     YVRRNNPRVRLTEQMHGGGHERGMRSGTLNVPGIVGLGAACEIAGAEMEAESARLAAL
                     RDRLQEKLLAGLDRVRVNGSQAHRLPGNLNMTFEGVDSETLMMAMKDVALSSGSACTS
                     AKIEPSHVLRALGLDEEAAHSSLRFGLGRFNTEEEVDYVADRLIDAVKKLREISPAFA
                     DMT"
     gene            complement(11528..11989)
                     /locus_tag="VHE33_03335"
     CDS             complement(11528..11989)
                     /locus_tag="VHE33_03335"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HVT96511.1"
                     /translation="MLRLTKKADYGLMALKYLAEHADATSLSAKDIAEAYHIPPQLLA
                     KILQRLAKVGILRSHAGMNGGYSLLKPPREISAFEVIHAIDGPLFITSCGPSHSGCDL
                     TDSCTIKEPLSRVNDSISGLLKSISIADLADPHTEVTVQRHPGQELVTIQL"
     gene            complement(12083..12859)
                     /locus_tag="VHE33_03340"
     CDS             complement(12083..12859)
                     /locus_tag="VHE33_03340"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT96512.1"
                     /translation="MARPGPFAVYPSLRERVVAVTGGASGIGAAIVEAFAANGARVAF
                     LDLQQEAGNLLVEHLRAKAAHAPVFVPCDLTDIDALRDAFARIVEILGPVEVLINNAG
                     NDTRHSLEDVTPEFWDRTLAVNLKHQFFAAQAVIPGMRQLGRGSIINMSSIAWVIPST
                     NVPVYVTAKAAIVGMTRTLAHELGKDNIRVNCILPGAIATERQKRLWLTPEYEAEILA
                     AQALKRLIEPEEVARLALFLAADDSAAITNQSYVIDGGWV"
     gene            12979..14352
                     /locus_tag="VHE33_03345"
     CDS             12979..14352
                     /locus_tag="VHE33_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HVT96513.1"
                     /translation="MNRRSFLKVAGTATTQALLATSLHAAAEKDQPGTLLTPQQSEPE
                     KPVAANDHIQIALIGAGGQGQNDTKVAASVPGIKVVAAADCYDNRLAHCKELWGQDIL
                     TTRDYREILDRKDIDAVIIGTPDHWHKQASIDAMTAGKDVYCEKPMIHLYSDGPAMIE
                     ASRSTKRILQIGSQRVSSIIYAKAKEILASGAIGKLNMISAYWDRNSSIGAWNYTVPP
                     DASPETCDWPRFLGTAPKIPFNGEHFFQWRKWKAYGSGVAGDLFVHLFSGIHFITGAH
                     GPTRAMATGGLRFWKDGRDVPDVLLGLFDYPEGFNLALRVNFVDGGSEGEGFLFTGSE
                     GTIEIAGNTLTVNRVPRDKEPGLMIGSFTEAMQKEITTAYREKYPLVEPTEPARVGND
                     RYVVPRDYSDSYDHFKNFFAAIRSREPVVEDAVFGYRAAGAALLSNLSYEREAAVKWD
                     PEAMKIV"
     gene            complement(14357..15487)
                     /locus_tag="VHE33_03350"
     CDS             complement(14357..15487)
                     /locus_tag="VHE33_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HVT96514.1"
                     /translation="MTFRRILLVLILVTGFWYFLNHSGFRNDGSKPALTLTEAHAAPE
                     FTPAETNNISVYKRVVPSVVNITAGTVTLDFFMGLVPQQDQGSGFILDKQGHILTNYH
                     VVGESHNVQVQTHDKHSYKATVVGKDRMHDLALLQIDAPNLVPAVLATSRELQVGQQV
                     YAIGNPFGLSGTMTSGIISAIRSVRGGEGSAPIENAIQTDAAINPGNSGGPLLNSQGE
                     VIGINSMIAQNGAEQNAGIGFAIPIDTAKAVLSDFQKYGYARRPSLGVRTLPIGPDLA
                     EQMGLAAQYGVLIENVVPGGAADRAGLHGGNQVAYLGNIQISIGGDLIVAIDGQEITN
                     SQDISEIMNHHQPGDTVTVTFFRGRKKMTARVTLGEAHEQSA"
     gene            complement(15599..16255)
                     /gene="coaE"
                     /locus_tag="VHE33_03355"
     CDS             complement(15599..16255)
                     /gene="coaE"
                     /locus_tag="VHE33_03355"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HVT96515.1"
                     /translation="MLRVGLTGSLGSGKTTVAAILRERGLHVLEADAIARAMMEPGQE
                     VFRGIVHHFGADVVRPDGSLNRPRLAALAFSEGRLNELNQIVHPPVIREQERMSREIF
                     ARDPDAIVVVESALIFEAEAWGTVPDWRKRFDRILLVTAPDDEKIRRFLARILPASAT
                     AEERAAAERDARQRLAAQLPDSLKIPQADFVIDNAGSFEATRDQTVRIAEDLRTHCGP
                     "
     gene            complement(16375..16530)
                     /locus_tag="VHE33_03360"
     CDS             complement(16375..16530)
                     /locus_tag="VHE33_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96516.1"
                     /translation="MTNLQENQSDEATPPHHAGPSWGLMLALIFLAMLVATGIAWMFI
                     HPFFTRH"
     gene            16729..16971
                     /locus_tag="VHE33_03365"
     CDS             16729..16971
                     /locus_tag="VHE33_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96517.1"
                     /translation="MASDSRIEKFPVTELAGLRTELLQSGMDSWQAAELVKTFLTGHG
                     YGADPVMIREAVLRLETHSCSVECMQSELEQVAYVM"
     gene            complement(17121..17708)
                     /gene="frr"
                     /locus_tag="VHE33_03370"
     CDS             complement(17121..17708)
                     /gene="frr"
                     /locus_tag="VHE33_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HVT96518.1"
                     /translation="MAPSVMAGIPALKADSAQLRTRMDKAVADFQTNLGSLRTGRASI
                     HMLDQVKVDYYGTPTPLNQVGQITTPDANMIVVAPWDASLIAEIEKALRTSDLGFNPN
                     NDGKVVRIPVPPMTEDRRREVVKHLHRVLEDHRTAVRNIRRDGNDLIKKAAKDKKISE
                     DEEKRSLDEIQKLTDEEIRKMEEMAKKKEAEIMQV"
     gene            complement(17798..20077)
                     /locus_tag="VHE33_03375"
     CDS             complement(17798..20077)
                     /locus_tag="VHE33_03375"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HVT96519.1"
                     /translation="MPVRGRQGTMVLPLLDGEPKLADFLSGAVRSPAAAQMLRISHFV
                     ERSPSDGSRPSEDTVAYLGYTHEAFFAAFICQDSRPQFIRAHMLARDAESDDDDVFMM
                     LDTFHDQRRAFLFQSNALGIQTDGLYSEQTGYDFSFDTVWDTWGKRTPTGFVVLMRIP
                     FASLYFANADPGEARTWGIILGRNISRTNESDYWPRVQHDIAGRLTQDMPVEGFEDIQ
                     HGQNLQFQPYSLARNMRQLNSVDPVNPFFEDKSFQAYSGMDAKFILHHSLVLDTTINP
                     DFSQVGIDNPATPNQRFPPYFAEVRPFFIENSSYFQTPISLYYTDNIVKPQFGARLTG
                     KLGPWAMGVLSVDDRSPGQLVPASDPNANKRAEFYATRVNRDLGKQSNIGLIYADREY
                     LGSFNRAGGFDYRARLGNNWTVTGQAVTSQTKNLSNDTPGEQYCETYALTCSGQAYFQ
                     QISYSDLHWGWWLAYNDNSAGFITDTGFFQRPDVREPNGRISYTFRPANGPILSHGPS
                     LYAERIWDHSGVALDAYANPSYSVTFKGRTTVSGWAQLGQDRLRPIDYSALPSNVEYP
                     SNQAGFNFYSSPVPYLAIGGGYYQGTAINYSPPDDQGPGPVDVSSPHLNVEVKPFGSV
                     DLQNSYVYTHFTDPATGNVVYDNHELISRWNYQLTKAASFNLIGQYISTLPHTEYTDA
                     ANSKTLFADALFTYMPHPGTALYVGYIGNFANLDRALCTREQDGLCNTANPILPPTGS
                     SLMNDGKNLYVKMSYLLRF"
     gene            complement(20403..21362)
                     /gene="lipA"
                     /locus_tag="VHE33_03380"
     CDS             complement(20403..21362)
                     /gene="lipA"
                     /locus_tag="VHE33_03380"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HVT96520.1"
                     /translation="MASPSAGNPSIPGEALIQIDLAPRRPSPKPEWLRARAPMGDNYH
                     DLKKLARSLNLHTVCESAQCPNIGECWHHRTATFMMLGNTCTRRCGFCAVPKGRPDPI
                     DFDEPRRVAEAVAALGLKFAVITSVNRDDDVVGGARAFVMVIDEIRRQAPGCQVEVLI
                     PDFQGNEEAIRLVVDARPEILNHNTESVPRLYRAVRSGARYERTLRLLQFAKEVEAKT
                     VTKSGVMVGLGEEMDELLQVYRDLAAVGVDVLTIGQYLRPSKDHLPIARYYTPDEFAF
                     MKREALAMGFRHVESGPLVRSSYHAHEQADSQAGVAGRTVVLH"
     gene            complement(21426..21701)
                     /locus_tag="VHE33_03385"
     CDS             complement(21426..21701)
                     /locus_tag="VHE33_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96521.1"
                     /translation="MTQLEALYRYEGHPTETIMFALGNLREVYGIRRIKVDEEWKTIR
                     VEYDATRLASPTIFQLLRRTGLAVVEELPLIPPQEAAPAEPATPAKS"
     gene            complement(21794..23827)
                     /locus_tag="VHE33_03390"
     CDS             complement(21794..23827)
                     /locus_tag="VHE33_03390"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HVT96522.1"
                     /translation="MAKHTVSRRTFLSLAGATAAAPPLMAATRAWGFDEPVTSAAPGW
                     HDQGVLNLAQSPHAKLRNIPVRAVTIEEGFWSKRRKTNVESSIPSMHQELLDHGRMDN
                     FLRLVGKSNEPQKGPVYSDSDMYKWLEGVAFALQTEDNPQLHEAAQTTIQQIVAVQEP
                     NGYLDTYYQDDRKSLRMTIPEQVRGHELYCLGHMLQGAIAYYRATGDPTLMNAGRKMV
                     DDYILPNFGPEPDKKPIVSGHPEIEMALIELYRTTGERKYVDLAGYILQGDPRANLRP
                     QQIVYMNCGVPFTTKTKLQGHAVRAMYANCGATDYYLETGDAAYMKTLEVLWSDLTRA
                     QMYINGGVGARAEGEAFGEDYELPNAQAYGESCAAIGNAMWNWRMLAATGEARFTDVM
                     ERALYNGINSGMSLKGTLYCYRNPLAFDPTSGEKIRNPWYDTTCCPPNLERTFASLPG
                     YFYSTSKDGLYVHLYDNSRLQWKLESGNPIVVTQKTQYPWEGAVRMTVAVEHPEEFTL
                     YVRVPAWSRESRVAVAGKPVEGVRPGEYMPIRRRWSGETPVELSFDMTPRMVVANPAV
                     EQDTGKVAMERGPVVFCMEGLDQTKGTDAKNFPLYMARRGGAIRGEYKADVLDGVMVL
                     THEGARRAEPVESLYAPAVAPAQVEAAELKLIPYYAWDNRDESSMQVWVPVAG"
     gene            23955..>25942
                     /locus_tag="VHE33_03395"
     CDS             23955..>25942
                     /locus_tag="VHE33_03395"
                     /inference="COORDINATES: protein motif:HMM:NF036483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96523.1"
                     /translation="MDHRRSFFDPRSTGMVRVRGAREHNLKDISVDIPRDALVVFTGV
                     SGSGKSSLAFGTLYAEAQRRYLESVSPYARRLFHQLTVPEVDSIDGLPPAVALQQQRG
                     SPTTRSSVGSVTTLSNLIRMLYSRAGDYPPKAPMLYAENFSPNRPEGACPKCHGLGRI
                     LTVTEKSMVPDPSLTIRERAVAAWPTAWQGQNLRDILVSMGYDVDRPWRDLPKKDRDW
                     ILFTDEQPTVPVYAGFTPAETRRALRQKMEPSYMGTFTSARKYVLDTFSKSQSERMKR
                     RVSEFMLAAECSVCHGKRLRADALEVQFAGLDIAALSALPLRQVHARLAPYASAKNSP
                     LRVTPERAAVAQRITQDIVARLDALLLLGLSYLSLDRSTPTLSPGELQRLRLATQMHS
                     NLFGVVYVLDEPSAGLHPSDTETLLHALDALKRSGNSLFIIEHEVDVIRRADWIVDVG
                     PGAGDKGGEILYSGPPAGLRNVEASKTRLYLDARTPPRRTSRSPSGWLRLRGATRNNL
                     HNLDVDFPLGVFTSVTGVSGSGKSTLVSQVLVELASRHLGQRVADEESAGEDRLTAEK
                     PILTGGSIESGMESIRRLVVVDQKPIGRTPRSNMATYTGLFDHVRALFASTRVARERR
                     YDAGRFSFNVAKGRCPTCSGEGFVCVELLFLPSVYSPCP"
BASE COUNT         4621 a         7892 c         8293 g         5136 t
ORIGIN      
        1 tggcgcagat gtttgcgcag cgcgagatgc ggaagacgta tctcgcactg gtacacggcc
       61 acctgaagca ggatcgaggc acgatcaatg cccccattgc ccgcgacttg ttgcgtcgca
      121 cacgcatgac cacgcgtcga caagccgggg cacggacggc gatctcgcat tacacggttt
      181 tgcgacggat cgcctcgcgg tttggaccgc tcaccctggt ttccgtgcgg attgaaaccg
      241 ggcgcacgca tcagatccgc gtgcatctgg cttccatcgg acacccggta gtcggagata
      301 cgctgtatgg cgcgcccgcc cgattgatgg tcattccagg gcggcgaaca gccgatccgg
      361 agccagagag cctcgcgttg aaacgaaact ttctgcatgc tgcggagctg gaattcgcgc
      421 atccgcgaac cgggaagcca atggctctgc agtccccgct gccagctgag ctggaggaac
      481 tgctgagcca aatcgagtca tccggcgccg cgaagcctct gacaccttga ctatctacaa
      541 tcgttattgt agatagtcat agtatggcga agccaagcga ccttgtgcag ggcactctcg
      601 accttctcat catgagaacg atttcgcctg agccgatgca tggctgggcg attgcgaagc
      661 ggattcagca gatctcgaat gaagtgctcc aggtgcagca gggatcgctg tatccggccc
      721 tgcaccgtct ggaacaacag ggctggattc gggcgaagtg gatggagagc gaaacgggcc
      781 ggcaggcgaa gttctattcg ctgacggcgg ccgggaagaa gcagctggaa gaggaaaggg
      841 ccaactggag ccggctgtct gcggcgatcg atctggtgat tgagaacgcc tgaggactcg
      901 ccgtccaggc gaacgcgctt cgcgcagagc gaatggacgg agcaggggaa gaacgatgcg
      961 aattctggaa aagcttcgaa tgcgcgtgca gatgctgctg caccgcgagc gcgaatcaga
     1021 ccggctgaat gcggaactgg agttccatct cgagcagcag attgcggaaa accgcgccgc
     1081 gggcatgagc ccggacgagg ctcgcgcggc cgctctgcgc agctttggca atccggttgt
     1141 gctgcgggaa caggcgcgcg aaagctggag ctggaactgg gcggaggcgc tgtcgcgcga
     1201 tattcgcatc gccacgcgca cattgcttcg gtcgccgggc ttcgcaatga ccgcgatcgt
     1261 cgtcatggcg ctgggcatcg gcgcgaacgt cgcgctcttc acgctggtac gctccgttct
     1321 gttgcgcccg ctgccttttg ccgccccggg gcagttggtc ggcatcttcg aagcgaaggc
     1381 ggacggcagc tttcaggaca acatcgtcgc cggcggctgc ttcgggagat ggaaagagca
     1441 atcgcacagc tttgccgcgc tggcccttag ccagagcacg agctacaacc tggccggaac
     1501 cggcaaccaa ctgcccgagg ttgttgaagc gcgcacggca agctggaaca tgttctcgct
     1561 gctgggcgtg cagccggcgc tgggacggtt ctttaccgaa acagaagacc agcgcggcgc
     1621 gaacgcgacg gcggtgctga cgtggggatt gtggaagcgg cgcttcggcg gcgatgcagg
     1681 catcgtaggg cgaaccattc tggtggatgc aaaaccatac acggttctgg gcatcctgcc
     1741 tgcgtggttt acgtggcccg atccgcgagt gcagctttgg acaccgctct atcacgagaa
     1801 gtcgcccgag atgatgcgca tgttcgacgc gcatagcttc gatgccgtag gccggctgcg
     1861 cccgggcgtg accatgcaac aggcgagcgc agaactgaat ggaattcagc ggcagattcg
     1921 gcgcgagcac ccggatgggc ccgtcaacga tgccgtaaat ctgcgctcgc tgattgacgc
     1981 cgatacctac gacatcaagg gcggcttgta tgcgctgctt ggcgctaccg gatgcctgct
     2041 gctgattgcg tgcctcaaca tcgcgaattt gcttgtggca cgctcggccg cgcgcagtaa
     2101 ggaaaccgcg attcgtaccg cgctcggggg cagccggctg cgcctcttgc gcgaacaggt
     2161 cgtcgaaagc atgcttctgt cgctggcggg aggtctgatg gggctattgc tggcgagcgg
     2221 aatcctgcac tggctgatca gcacacgcga agacattccg cgggccgact cgatccatat
     2281 ggacggtgtg gtggcgctgt ttacgctggc ggcggttctc ggctgtggca ttctggccgg
     2341 cctgattcct gcattgggcg cacgcgatgc tcatgtgctg acgacgctcc aggaattatc
     2401 gcgcagctac agcggtggac gcagccgtgc gcgtgttcgc caatttctgc tggcgcttga
     2461 ggtggctctc acagttgtcc tgctgatcgg agcgggcctg ttgctgcgca gctatcagca
     2521 actgcggtcg gcggacctcg gcttcgcgca gcacaacatc ctcaccatga ggatcacctt
     2581 gtcgcgcaac agttatccaa ctaacgcgag ccgcgtggcg ttcttcgacc agttgttgag
     2641 ccgcgtgcgc gcgctgccgg gcgcacaggc tgtgggactg accagcgctc tccccggcga
     2701 gggccgccag cgcaatgatg tttttgtcat cgctgaacat ccgccgcttc cgcgcggtca
     2761 ggtgatcgat gcgctgactc gattcgtcga tccgggatat ttcaaggcga tccagatccc
     2821 tctgattgag gggcgcacgc tggagcccag cgagcgcctg gaccagtcgc aagtggtggt
     2881 cgtgaatcag gagctggtga aggaatattt tcccaacgaa gatccgatcg gacgccacgt
     2941 gaaggccgag atggtcctgg gtacgacgcc gctgcgtatt gtgggcgtgg tgggcaacac
     3001 gcgcgatgca atctcccacg atccgtttcc ggtaatctac tacccgctct tcagcggcga
     3061 tcagcgtggc gtatcgctgg tggtgcacag ccggcgcgat cccgccggaa tcgcgctctc
     3121 cgttcagaaa gcggtcgcgt cgatcgatcc gcagctgccg gtctccgatg ttcttacgat
     3181 ggaagaggtg cttggcgggt caacgctcga tgcgcgtttc gacgcaattc tgctcgtcgc
     3241 cttcgcgatc ctgtcgatgt tgctggccat ggtgggcctc ttcggcgttc tgtcgttcat
     3301 ggtggcgcaa cgcacgacgg aaatcggcgt acgcattgcg ctcggagcga gccgcgaaaa
     3361 catcatgcgc aagatgctgc gcgatgggct gcgccccgcc atctatggac tggttctggg
     3421 acttgcggcc agcatgggag cggcccggct gatccagtcg ctgctctttg gtacaaaacc
     3481 attcgatccg gcggtgttcg tgctggtgtc ctttacgctg ctgctggtgg cggcgctggc
     3541 ctgcgctctg cccgcctggc gcgcgtcacg tctggatccg attcaggcgc tgcgcgcgga
     3601 ataggacctt catcagcaag tcggcggtcc gttatcatcg gctgagaacg acctggagga
     3661 ttttcgccga tgcaggttct gtacaccgcg gatgtaacgg ccgaaggcgc gcgccacggc
     3721 catgtacgca gctccgatgg gctgctggat ttcgatctcg cgctgccgcc gggcatgggc
     3781 ggcaagggcg acaagacgaa ccccgagcag ctgtttggag cgggctacgc ggcgtgcttc
     3841 ggcggcgcgg ttgagtatca ggccatgctg gcgaaaaaga agatcggcgt ggtcacggtg
     3901 cgcgctcacg tgagtgtcgg accctttgac gaggggcagg gtgagggatt cacgctgcgc
     3961 gtcgaactgg acgtaacgat tccggaagtc gatcaggcga ctgccgagga gctggttgcg
     4021 gcggcgcatc gaggatgccc ttattcgaat gcgacgcgag gcaacatcga agtgaagctg
     4081 accgcgcacg gcggcaggtg agcagaacca aagcagcggt caccctgcag catcaggaag
     4141 tgcggctggc gcgattcgcg catgtctgcg accttcctgc gtactatggc tgaatgcaga
     4201 gcgaacgaag agccacagca tttctggcgc tggccggagg agtcatctcc atctcgtggt
     4261 cggcgatctt cgttcgctgg gcgcgcatgc cgggaacctg ctcggccttc taccgcacac
     4321 tcatcgccag tgtcgtgttg tggggaatgt ttccttttct gcgcgacaag agccggcgcc
     4381 tgtcgcggcg atccttctgg atcacggtcc tgggcggcgc gctttttgcc ggcgatgtag
     4441 ggctgtggaa cacggccgtg atgcacactt cggcggggaa cgcgacgttt ctgagcaaca
     4501 ttgcgccgct gttcgtcgga ctcttcacat ggatcttcac gcgccatctg ccttcgcgac
     4561 ggttctggat tgctttgctg gtcagcaccc tcggcagtgg gctcatcgtc gccgccgatc
     4621 tgcgccttgc tttgggccgc tcgtctgcag atgggcttgc cctgctggcg gcggtttgct
     4681 tcgcgctcta cctgatcatt accagccgcc tacgcgagaa acacgacgtt ctggaactcc
     4741 tggcgctttc gacgacggcg agtgcggttg tgcttctgat cgccgcgggt gcagctcgct
     4801 tgccgatgaa tattccgagc gccgggtcgc tgtgggcctt gctggcgctg ggcctgatct
     4861 gccaggtggg cggctatttc ggactcacgt atgctctggg gcatttgcct gcgaccgtga
     4921 cgtccatcct ttttctgagc gtcgcgccca tgaccgcgat ctttgcctgg atcatcttcg
     4981 gcgagcgcat gagcctggtt gaaattgccg gcggcgcgct gattctgagc ggcgcatgga
     5041 tcgtgggccg aaaagagaag cctgaggttc cgcaggtgga agagctggcg ccgcccgtgg
     5101 ctgaggctta ggccgctaca gcttctgcga acgctggacg tcgcgcacgc cggggagtcg
     5161 ccgtaaagcc tgcaccagct tcgtcaggtg cttcacgtct tccgcgtcga tcacgaactg
     5221 aatctgcgcg tcgcctgcgt caccgagatg cgtctccatg gttcggatgt tcgcattctc
     5281 gtcgctgatg acggcggtga tttccttcag cattccggcg cgatcgtcgc agtgaacggt
     5341 gaaacgcacg ggataggtct gcttgtgtcc ggctttgtct tcgggcgagc gcgcccactc
     5401 gacggcgatg cgccggtcgg cctcgtagag caggttctga acgtttggac aggtacgcgc
     5461 gtgtaccgcg actcccttgc cgcgcgtcac gtagccgatg atttcttcgc cgcgtatggg
     5521 attgcaacag cgcgcgcgat agaccagcag ctcattctgc ccctcgacga tgagggaatc
     5581 ggtaccgctg gcggcggccg cacgacgcac cgcatccgcg ccaggactgg ccggcgtggc
     5641 tgcagcttcg ctctcctgct gctgatggat cagccctggg gccaggcgat tcagaacctg
     5701 acgcgcggaa tacttgccga atccaatggc agcccagaga tcgttgggcg tcgccacgct
     5761 gtaatcggcg gccacgcgcg catagtccga ttcctcaaac tgcgacagtc ccaccttgta
     5821 cttgcgcgcc tcgcgctcca gcagcttgcg cccgatttcg atggcgcgtt cgcgctggtg
     5881 ttcgttgagc cagtggcgaa tcttattgcg cgcgcgcggg cttttgacga aggtgagcca
     5941 gtcgcggctg ggcgtgtgcc cgttctgggt gatgacctcg acgatatcgc cgttgcgcag
     6001 tttcgtccgc aacggcacca tgcggccgtt gacgcgggcg ccgacgcagg tattaccgac
     6061 ctcggtgtga atcgtataag cgaagtccac gggcgtgccg tcgcgcggta cgacaaccac
     6121 tttgcccttc ggggtgaagg tgtaaacctc ctccggatac agatcgatct tcagcgtcga
     6181 caggaactcg ttgggatcgg tcatgtcgcg ctgccactcg actagttgcc gaacccatgc
     6241 caggcgctgc tcgtcgcgcg cgctgacggg ggagccgccc gccttgtatt tccagtgcgc
     6301 ggcgatgcct tcttccgcga tgcggtgcat ctcctcggtg cggatctgca cctcgaactg
     6361 gtggccgccc tcacccatca cagttgtgtg cagcgactga tacaaattcg gccgcggcat
     6421 ggcgatgaag tccttgatgc gaccgggcac cggacgccag atactgtgga tgatgccgaa
     6481 aacggcgtaa cagtcctgca cgctggtggt gatcacgcgc agcgccagca ggtcgtagac
     6541 ctggtcgatc gaggtctgcg acttcaccag cttgctgtga atgctgtaca ggcgcttgat
     6601 acgccattcc acatgtgcct tgatgccgtt ctcgtgcaga cgctcgctga gcaatccctc
     6661 cacgccggcc aggaattgtt caccctcgga gcgcctcgct tcgatcgctt cgtgcagctg
     6721 ctcccaggtg atgggatcga cccagcgaaa ggccaggtcc tccagctcgc cgcgcagttt
     6781 gcccatgccc aggcggtgag cgagcggagc gtagatgtcg agggtctcgc gcgcgatggc
     6841 acgctgccgt tccggcgcca ggtgctccag agtacgcatg ttgtgcaggc ggtcggccag
     6901 cttgatcagt accacgcgga cgtcgctcac cattgcaagg agcatcttgc ggacgttttc
     6961 cgcctgacga tcttctcggt tggcaaactg aatcttgtcg atcttggtga caccctcaac
     7021 gatgtgcgcc acctgctcgc cgaaccgggc gacgatgtct tcggtggtga caggcgtgtc
     7081 ttcgaccgcg tcatggagca ggccggcggc gatcgccgtg gagtccagtt tcatctccgc
     7141 cagaaccagc gccacttcca gcgggtgcag gacatagggc tcgcccgagg cgcgcagctg
     7201 gccattatga tgttccaggc agaactgcca ggcctggcgg atgatgttga cgtcgtcgga
     7261 gggccggttt ttgtgaaccg tgtccagcag cttgccgaac ttctcccgga acaggtcagg
     7321 gacccgctca acctggagcg cggagggctg gacactcgcc atatcccatt ataggcggga
     7381 gctgcaagag gggcatcaaa ctacaggcca cagggcacag gctctgtcct attccggctt
     7441 ccccggattc tttggcttcg gcggcgtctt cacgggcggt ggcggactgc ccggcagcag
     7501 tcccggcgtg ggccgccggt tctcgtggac ccaggcgagc agctcatcaa aggcgagacc
     7561 ggtttgatcg gctgtgacgt tgcagtggcc gtcgcgatcg acatactgct gcacaaaatt
     7621 gccgccgttt cccacccggc gggtcaactc gtcgtaccag gccgtggtcg aagacggcac
     7681 caggggatcg tagatggtgt ggacttccag cagcttgcgg tgcaggttcc cggttggcga
     7741 gtaccagtct gcgagaaaac gataggcgtt tggatcggac gcgtagcggt ggacgcgctg
     7801 gttgagcagt gcgtcgttga ctgtcccgct gtacaccaga ttgcggttgt cgaagggatt
     7861 gccgcccgac ttgagccgca ggtcctcgat cacgaatgtg ttgaagacca gcacgtcggc
     7921 gagcccgcga ttcgtgtgga tattagcgat ctgtcgcacg agcgcggcct ggtccggatt
     7981 ggcatcgagg ctcttctgga ttttggtgat ggtcgggatg tcattcatcg cgaaggtcgg
     8041 cgggatgtgc tccacatccg ggagtgctcc aggaaagaaa gcgtcaaacg cgacgcggtt
     8101 ggcaaacgcg cgctgcatca cgagatcggc gggcgccagt acaccgcaca acgccagccc
     8161 cccggcatat tccttcgggt tctgttccag tcccatgagt gtgagcaggc cgcccatgga
     8221 gtgacccatc agcaacgtct cgcgcggctg gccatatttc ctgcggaagt aatggcgcaa
     8281 tgcttcggtt tcggcggctg cctggggcac ggcccatccg cccaccgcat atccggagcg
     8341 cgcgaccgca aagccgcggt tcaggatctc ctgcagcacg cgggttgggg gatactttgg
     8401 atcgaaaacc tgcggcgtct gctgatagcc gtgaaagtag aggaccaaac cgtgattcca
     8461 gtccttcggg acgtcgatgc ggaacgaggc gccgttgatt ttgccgattt ccgtcgtcac
     8521 gggaacggct ggagggccgg aaggtgcaga ggtcgtgttc gctgaaaccg gtgccgggcc
     8581 aggtgcagat ttttcagtcg ccgtcgcagg tgcggatggt gaagagcgac tattcggtgc
     8641 gccctgaccc ggctgcggag ccggagcgga tgcggaagcc gccccctgct ggcttcctgc
     8701 cgactggctg ctcactcctg ctggattttc cccgctcgtt tgggcgagcg ctccagcagc
     8761 gagcaccgtc agcatcagca cgagggcaaa gcgcgggaga aaccccttca agtgcgatcc
     8821 tctccactgt gcgaagatga gtttttgccc gagcgatcac cggggctggg caaaacctgc
     8881 tgctgcaaaa ttctgatgcc gactcccacc aaacgcgaat caccccgaaa gaaacaagct
     8941 acccgaaaag ctacgccgat ggcttcggga aagcaggctg aggcgaacca tggcacgcac
     9001 tcccgccagg atgcgataca aaccagggaa ttccagccag cggttcctgg cgccattggt
     9061 atctctcgcc gagctgcgga ccgtctgcgc gccggccacg tctgggtcta tcgctccgac
     9121 atcgaagcat ggcccgccac gccggatgcg attcctgccg ggcttgtgcc ggtcacagat
     9181 cgtcgtggcg ctttgctggg taccggcctt tacagcccgg cttcggaaat tgcgctgcgg
     9241 ctcgtctcga cggacctgat ccagactgaa gcggagtggc ttgatttgct tcggtcgcgc
     9301 ttgcaggcgg ccgtggcgcg tcgcgggccg cttctcgacg ctgagaatca tgcgtgccgc
     9361 ctcgtcttca gcgaggccga cgagcttccg ggcgtcacgg tcgaccggta cgccggactc
     9421 atgatcgtcc agctgaggca caaggcgctc gatacagatg ccacccgggc cgtggtcacc
     9481 gaaacgctgc gcaatgcgct cgatccggag acgggcgaac tggaaacgat ccttgagcgg
     9541 gaggacccgc gcatccgcga actggagcag ttgagtgctc ccgcttcttc gacgctgttc
     9601 gcgcgcgacc cggagcatcc cgcgacgcgg accgtcttcc gccttaacgg cctccaattc
     9661 cacttcgacg ccaccagcgg ccagaaaacc ggcgcctttc tcgaccagcg cgaaaactac
     9721 gctgctgccg cgcgttacgc agccggagaa gcgctcgatg tgtgtaccta tcagggtgga
     9781 ttcgcgctgc atcttgcgcg cgtttgcccg cgcgtcacgg gtatcgatgt ttcccgcgcc
     9841 gcgcttgagg tcgctgagca gaatttggct ctcaatcgag atctgctcgg gaactgcgca
     9901 gtggattggg tggaggcgga cgtctttact ttgctgcgcg actggagcga tgcggggagc
     9961 gtctatgaca cgatcgtgct cgatccgccc gccttcgcaa agagcaaacg cgcggtggag
    10021 ggcgccctcc gtggctatcg cgagctgaac ctgcgcgcgc tgaaaatgct gcgtcccggc
    10081 gggcggctca tcacctgttc ctgctcgcac catgtttcgc tggccgatct catggagact
    10141 ttgcgtgcag cggccggcga tgcccgccgc cgtgtgcgtc tgttggaggt gcgaggcgcc
    10201 tcgccggatc atcccgtgat cctgaatatt ccggagacag agtatctgaa gtgcgtgatt
    10261 ctcgaggcgg agtgagggtt cgggcgacct ctggatgcgc cgctcatgtc atatcggcaa
    10321 aggccggtga aatctcgcgc agctttttga ccgcatcgat cagccggtct gccacgtaat
    10381 ccacttcttc ttcggtgttg aagcgtccca gaccaaagcg gagggagctg tgcgcggcct
    10441 cttcatcgag ccccagggcg cgcagcacgt gcgacggctc aatcttcgcc gacgtacacg
    10501 ctgagccgct cgacagcgcc acgtccttca tagccatcat taacgtctcg ctgtccacgc
    10561 cctcgaaggt catattgaga ttgccgggaa ggcggtgtgc ctgcgatccg ttcacccgca
    10621 cgcgatcgag gccggcgagg agcttttcct gcaggcggtc gcgcagggcc gccagccgcg
    10681 ccgattcggc ctccatctcg gctccggcga tctcacaggc tgcgcccagg cccacgattc
    10741 ccggcacgtt cagcgtgccc gaccgcatgc cgcgctcgtg gccgccgcca tgcatctgtt
    10801 ccgtgagtcg gacgcgcgga ttgtttcgcc gcacatacag cgcgccaatg cccttggggc
    10861 cgtagatttt gtgtccggag atcgagagca gatcgatgtg gctccggttt acgtcgatgg
    10921 gaattttgcc caccgcctgc gccgcgtcgg tgtggaacag cacgccgcgc tcgtggcaca
    10981 acgcgccaat ctcattgacc ggctgaaggg ttccaatctc gttgttggcg gcgaggattg
    11041 tcaccagcag cggcttttct tcccccccag cttcattcaa agcccgctgc aaatcagcga
    11101 tccgcacacg gccctcgccg tcgaccggca gcaccgtcac acggcagccg cgtttctcga
    11161 tccatacaca cgggtcgagc accgctttat gttccgtggc ctgggtgatc acggatcctc
    11221 ggctgccctg cgcttccacg gcgccggtga tcgcgaggtt attgctctcc gttgcgcccg
    11281 aggtgaagac gatttcccgc gccgtagctc cgatcagctt cgccacccgt gcccgcgccg
    11341 tctcgaccgc ctgttccgcc gcccagccaa agccgtggct gcgactcgcg gcgttgccgt
    11401 aggcctcggt caggtacggc atcatcgcat ccaggacgcg cggatcgatg cgcgtggtcg
    11461 cgtggttgtc catgtagatg ggcagtttca tggtgggtcc tgagcgttag aggcgcctgc
    11521 tttgagctta cagctggatt gtgaccagtt cctggccggg gtggcgttgc accgtgactt
    11581 cggtgtgggg gtcggccagg tcggcgatac tgatgctctt cagcaggccc gagatgctgt
    11641 cattcacccg cgacaagggc tctttgatgg tacagctgtc ggtcaggtcg caaccgctgt
    11701 ggctcggccc gcacgaggtg atgaacagcg gcccgtcgat cgcgtgaatc acctcaaacg
    11761 ccgatatctc gcgcggcggc ttcagcagcg aatatccccc gttcatgccg gcgtgcgagc
    11821 gcaggatgcc gaccttcgcc agccgctgca ggattttggc cagcagttgc ggcggaatgt
    11881 ggtaagcctc ggcgatgtcc ttggcgctca atgaggtggc gtcagcgtgc tcagccagat
    11941 acttgagagc catgaggccg taatcggctt ttttggtgag cctgagcata gtgcgcccgt
    12001 atagcaagtc tacggggaag aggaagcgaa ttcaatgcga ctggcaaatg cgcgctttga
    12061 acggcttccg aggccttccg ggctacaccc agccgccgtc gatcacgtaa ctctggttcg
    12121 tgatggccgc gctgtcatcg gctgccagaa acaaggccag ccgtgccact tcttcgggct
    12181 cgatcagccg cttcagcgcc tgcgccgcca gaatctccgc ctcatattct ggcgtgagcc
    12241 acagccgctt ctgccgctcc gtcgcgatcg cgccgggcaa aatgcagttc acgcgaatat
    12301 tgtccttgcc cagctcatgc gccagcgtgc gggtcatccc gacgatggca gccttggccg
    12361 tcacgtacac cggcacattc gtcgagggaa tcacccaggc gatggagctc atattgatga
    12421 tcgagccgcg ccccagctgt cgcattccag gaatgaccgc ctgcgccgca aaaaactgat
    12481 gcttcagatt gacggcgagg gtgcgatccc aaaactccgg cgtgacgtct tcgagagagt
    12541 gccgcgtgtc attgccggcg ttgttgatca ggacctcgac tggtcccagg atttcgacaa
    12601 tcctggcgaa tgcgtcccgc agtgcgtcga tgtcagtgag atcgcacgga acaaaaacag
    12661 gagcgtgagc ggcctttgcg cgcaaatgtt cgaccagcag gttgccggct tcctgctgca
    12721 gatcgagaaa agcgacacgc gctccgttgg cggcaaatgc ctcgacgatt gccgcgccga
    12781 tgccgctcgc gccgccggtg accgcaacga cgcgctcgcg caggctggga tagacagcga
    12841 agggtccggg acgtgccatg gcgtatcgat gatacgacgc atcttttcgt gcttcgaccg
    12901 tgactccggc cagggagctg gaaaaggatt tatctttgca aggagtcgat tcagcgactc
    12961 ggggaggtcc tgacacaagt gaatcgcaga agctttctga aggtagccgg aaccgcgacc
    13021 acacaggcgc tgcttgccac cagccttcac gccgctgcgg agaaggacca gccgggaacg
    13081 ttgcttacgc cgcagcagtc cgagccggaa aagccggtcg ccgctaacga ccacatccag
    13141 attgcgctga tcggcgccgg cggccagggt cagaacgaca cgaaggttgc ggccagcgtt
    13201 cctggaatca aggtggtcgc ggctgccgac tgctacgaca accggctcgc ccattgcaag
    13261 gagctttggg gccaggacat cctcaccacc cgcgactacc gcgagattct cgaccgcaaa
    13321 gacatcgacg ccgtcatcat cggcacgccc gatcactggc acaagcaggc ctcgattgat
    13381 gccatgactg ccggtaagga cgtctactgc gagaagccga tgatccatct ctactcggac
    13441 gggccggcga tgatcgaggc ctcgcgctcg acgaagcgca tcctgcagat cggcagccag
    13501 cgcgtgagtt cgatcatcta cgccaaggcg aaagagatcc tcgcctcggg cgccatcggt
    13561 aagctcaaca tgatctcggc ctactgggat cgcaattcct cgatcggtgc gtggaactac
    13621 acggttccac ccgatgcgtc gccggaaacc tgcgactggc cgcgctttct cggcacagcg
    13681 ccgaagattc cgttcaacgg cgagcacttc ttccagtgga ggaagtggaa ggcctatggc
    13741 agcggcgtgg ccggggattt gtttgtccat ctgttcagtg gcattcactt catcaccggc
    13801 gcgcacggac ccacgcgcgc catggccacc ggcgggctgc gtttctggaa agacggccgc
    13861 gacgttcccg acgtgctgct ggggctcttc gactaccccg agggcttcaa tctcgcgctg
    13921 cgggtgaatt tcgtcgatgg cggcagcgag ggcgagggtt tcctcttcac cggctccgag
    13981 ggcacgatag agatcgccgg caatacgctg accgtgaatc gtgtgccccg ggataaggag
    14041 cccggcctga tgattggctc gttcacggag gcgatgcaga aggaaatcac gacggcttat
    14101 cgggagaaat acccgctcgt tgagcccacc gagccggcgc gtgttggcaa cgatcgctat
    14161 gtggtgccgc gcgactacag cgacagctac gatcacttca aaaatttctt tgcagcgatc
    14221 cgttcgcgcg agccggtggt ggaggatgcc gtcttcggct atcgcgcagc gggcgcggct
    14281 ctgctctcaa acctcagcta tgagcgcgag gccgcggtga agtgggatcc ggaagcgatg
    14341 aaaatagtct gaatcgtcag gcgctctgct cgtgagcttc gccgagcgtc acgcgcgccg
    14401 tcatcttctt tcgtccgcgg aagaacgtca ccgtgaccgt atcgcccggc tgatgatggt
    14461 tcatgatctc ggagatgtcc tgggagttcg tgatctcctg gccgtcaatg gccacgatga
    14521 ggtcgccgcc gatagaaatc tggatgttgc cgagatacgc gacctggttg ccgccgtgca
    14581 gaccggcacg atcggccgct ccgcccggca caacgttttc aatgagcaca ccgtactgcg
    14641 cggccagacc catttgctcg gcgagatcgg gaccgatggg cagcgtgcgg acgccaagcg
    14701 agggccgacg cgcatagccg tacttctgga agtcgctgag cacggccttc gcagtatcga
    14761 tgggaatcgc aaagccgatg ccggcatttt gctcggcgcc gttctgggca atcatcgagt
    14821 tgatgccgat gacttcgccc tgcgagttca gcagcggacc gccggagttg ccgggattga
    14881 tggcggcgtc ggtctggata gcgttctcga tgggcgcgct gccttcgcct ccacgaacgg
    14941 agcgaatggc gctgatgatc ccggatgtca tggttccgct cagcccaaac ggattgccga
    15001 tggcataaac ctgctggcca acctggagtt cgcgcgaggt ggcgagaacc gccggaacca
    15061 gattcggcgc gtcgatctgc agcagggcga gatcgtgcat gcgatccttg ccgaccacgg
    15121 tggccttgta gctgtgtttg tcgtgcgtct gcacctgcac gttatgactt tcgccaacga
    15181 cgtgatagtt ggtgaggatg tgcccctgct tgtcgaggat gaagccggag ccctgatcct
    15241 gctggggcac cagacccata aagaaatcga gcgtgacggt gccggcggtg atgttcacga
    15301 ccgacggaac cacccgtttg tagacggaga tattgttcgt ctcagcgggc gtgaactcgg
    15361 gcgcggcgtg cgcttcggtg agggtgagcg ccggcttgct gccgtcattg cggaagccgg
    15421 agtggttcag gaaataccag aaccccgtta caagaatcag aacaagcaga atgcggcgaa
    15481 aagtcataga gctgacgtta tgtttcgatg cgggctcggg cggcgccggc cggcgcagac
    15541 ttgcgccaac agccggacgc agcaacgtgg ccctgtcttc gattgtaacg acatacgttc
    15601 aggggccgca gtgggttcgg aggtcttcgg cgatgcgcac agtctgatcg cgggtggcct
    15661 cgaaggaacc cgcgttgtcg atgacgaagt cggcctgtgg aatcttgagc gagtcgggca
    15721 attgcgcagc gaggcgctgc cgcgcatcgc gttccgccgc ggctcgctcc tctgccgttg
    15781 cggatgcagg cagaatgcgg gccaggaagc gccgaatctt ctcatcgtcc ggcgcggtca
    15841 cgagaaggat gcggtcgaag cgcttgcgcc agtccggcac cgtgccccag gcttcggcct
    15901 caaagatcaa agccgactcg acgacgacga tggcatccgg atcgcgagcg aagatctcgc
    15961 ggctcatcct ctcctgctcc cggatcacgg gcgggtgaac gatctggttc aactcgttga
    16021 gccgtccctc ggagaaggcg agcgcggcca aacgaggccg gttgagggaa ccatcgggac
    16081 ggacgacatc ggcgccgaag tggtggacga ttccgcgaaa gacttcctgg ccgggctcca
    16141 tcatcgcgcg cgcgatggcg tcggcctcga gaacatggag gccgcgctcg cgaagaatcg
    16201 cggcgacggt cgtcttgccg ctgcccagac tgccggtaag gcctacccgc aacatacgtc
    16261 tccgatcgct acactcggca tgatgaagcc ttgtgtatcc gatggaattg taagacggag
    16321 cgcgggaatg aaccgcgagc gccggagaaa cgtagtggca gggtgcagca ccattcagtg
    16381 acgcgtgaag aaagggtgga taaacatcca cgcaatgccg gtggcgacga gcatggcgag
    16441 aaaaatgagc gccagcatca gaccccagga cgggcccgcg tgatgcggcg gggttgcctc
    16501 atcggactga ttttcctgca ggttggtcac cagttagtgt acggcgaatg gttaccccag
    16561 agaggcactc agcgcgagat ggcgcaaaag ggccacaagc agattcctgg gaggcagcaa
    16621 cactatttca cccaaacggt gtctaaccga agcagaggta aggggtcagg acgatcgaga
    16681 atgcactcgg gagaatcgcc ccgagcggat cgaaacgagg agacgaccat ggcgagcgac
    16741 agcagaattg agaagttccc agtgacagaa ctggcaggtc tgcggacgga gctgttgcag
    16801 tcgggcatgg attcctggca ggcggcggag ctggtgaaga cgttcctgac cggacacggc
    16861 tacggcgccg atccggtgat gattcgggaa gccgttctgc gcctggaaac tcactcgtgc
    16921 agcgttgagt gcatgcagag tgagttggag caggtggcct acgtgatgta atgcctgggc
    16981 gatgaccggc gcggactggg tcgcaaaatt aaaggccgtc tttgcggacg gcctttttgc
    17041 tgctcctcat gtccaccttg ccgccaaaat gaaaagagcc tgtccgggcg gacggctctt
    17101 ttcacaagca cagcttcgca tcagacctgc atgatctcgg cttccttctt tttcgccatc
    17161 tcttccatct tcctgatctc ttcgtcggtg agcttctgaa tttcgtccag ggagcgcttc
    17221 tcttcgtcct cggagatctt cttatccttt gcggctttct tgatgagatc gttgccgtcg
    17281 cggcgaatgt tgcggacggc ggtgcggtga tcctcaagga cgcgatgcag gtgtttgacc
    17341 acctcgcgac gccggtcctc ggtcatgggc gggacgggga tacgaaccac cttgccgtcg
    17401 ttgttggggt tgaaccccag gtccgatgtt cgcagcgcct tttcgatctc ggcgatcagg
    17461 ctcgcatccc atggcgcgac gacgatcatg ttggcgtcgg gtgtggtgat ctggccgacc
    17521 tgattcagtg gcgtaggcgt gccgtagtag tcgactttga cctggtcgag catgtggatg
    17581 gaagctcggc cggtgcggag ggaaccgagg ttggtctgga agtcggcgac ggctttatcc
    17641 atgcgagtgc ggagttgcgc ggagtcggct ttaagtgcgg gaatgcccgc cattacggat
    17701 ggcgccatag gattcgtcct caggaaacag gttcaaaaca ggattgtacg cgggttggcg
    17761 agccagttca cgacatgatg agtgcgaatg gggccgctca gaagcgcagc aggtacgaca
    17821 ttttcacgta gagattcttg ccatcgttca tgagggatga gccggtgggc ggcaggattg
    17881 ggtttgcggt attgcagagt ccgtcctgtt cccgcgtgca cagggcgcga tccagattcg
    17941 cgaagttgcc gatgtagccg acatagagcg ccgtgcccgg gtgcggcatg taggtaaaca
    18001 gcgcatcggc aaacagcgtc ttcgagttgg ccgcgtcggt gtattccgta tgcggcagcg
    18061 tcgagatgta ctgcccgatc agattgaacg acgccgcttt ggtgagctga tagttccagc
    18121 gcgagatcaa ctcgtgattg tcgtagacca cgttgccggt tgccgggtcg gtgaagtgcg
    18181 tgtacacgta gctgttctgc agatcgaccg agccgaacgg cttcacttcc acattgagat
    18241 gcggtgagga aacgtccacg ggccccggac cctgatcgtc gggtggcgaa tagttgatcg
    18301 ccgtcccctg gtagtatccg ccgccgatag cgaggtacgg cacgggcgac gaatagaaat
    18361 tgaacccagc ctgattgctg gggtactcta cgttactcgg cagggcggag tagtcgatgg
    18421 ggcgaaggcg gtcctgcccc agctgcgccc accccgacac cgttgttctg cctttgaacg
    18481 ttacgctgta ggaaggattc gcatacgcat cgagcgccac gccgctgtgg tcccagatgc
    18541 gctccgcata caggctcggc ccatgcgaga ggatgggccc gttcgccggc cggaaagtat
    18601 acgagatgcg gccgttgggt tcgcgcacat cgggccgctg gaagaagccg gtgtcggtta
    18661 taaatcccgc ggagttgtcg ttgtaagcga gccaccagcc ccaatgcaga tcggagtagc
    18721 tgatctgctg gaagtacgcc tgaccgctgc aggtgagcgc gtaagtctcg cagtactgtt
    18781 cgccaggagt gtcattgctc agattctttg tctgcgaggt cacagcctgg ccggtcacgg
    18841 tccagttgtt gccgagacgc gccctgtagt cgaacccgcc cgcgcggttg aaagagccca
    18901 gatactcgcg gtcggcgtaa atcaagccga tgttggactg cttgccgaga tcgcggttca
    18961 cgcgggtcgc gtagaattcc gcacgcttgt tcgcgttggg atcgctggcc gggactaatt
    19021 gcccagggct gcgatcgtcg acgctgagca cacccatggc ccatgggccc agcttgccgg
    19081 tcagccgcgc gccaaactgc ggcttcacga tgttatcggt gtagtagagg ctgatcggcg
    19141 tctggaagta gctgctgttc tcgatgaaaa agggccgcac ctccgcgaaa tacgggggga
    19201 agcgctggtt gggggttgcc ggattgtcga tgcccacctg actgaaatcc ggattgatgg
    19261 tcgtgtccag caccaggctg tgatgcagga tgaacttcgc atccatgccg ctgtacgcct
    19321 ggaaggactt gtcctcaaag aagggattga ccggatcgac agagttcagc tggcgcatat
    19381 tgcgcgccag cgaatagggc tggaactgca gattttgtcc gtgctggatg tcttcgaaac
    19441 cctcaacggg catgtcctgg gtcaggcgcc cggcgatgtc gtgctggacg cgcggccagt
    19501 aatccgactc attggtgcgc gagatgttgc gtccaaggat gatgccccag gtgcgagcct
    19561 cgccaggatc ggcgttggcg aagtaaaggc tggcgaaggg gatgcgcatg agtacgacga
    19621 agcccgtcgg ggtgcgcttc ccccacgtgt cccatacggt atcgaacgaa aagtcgtagc
    19681 cggtttgctc gctgtaaagc ccgtcggtct ggatgccgag cgcgttgctc tggaagagga
    19741 aggcgcggcg ctggtcgtgg aacgtgtcga gcatcatgaa gacgtcatcg tcgtcgctct
    19801 cggcgtctcg cgccagcatg tgagcgcgaa tgaactgcgg ccggctgtcc tggcagataa
    19861 acgccgcaaa gaaggcctca tgcgtgtagc cgaggtacgc gacggtgtct tccgaggggc
    19921 ggcttccgtc ggatggcgat cgctcgacga agtggctgat gcgaagcatc tgagcggcgg
    19981 ccggagagcg cacagcaccg ctgagaaagt ctgcgagctt cggttctccg tcgagcaggg
    20041 ggagaaccat cgtgccctgc cggccgcgta ccggcatgac ggcgctgctc gatcctgaac
    20101 tctcgctgga gtttgccgcc gggccccggg cagaggcgtg cccgttttca gcttcgcccg
    20161 gcttgcaggc gtgcgcgggc cggggcgcaa aggcgcccag ccaaagcagt aagagaaacc
    20221 aaaaccgcag agaatcaaaa cgcatgctgc acaaaactcg ttctccggtt caccggagaa
    20281 ggcaggaact cgctctcgtg cagagctcct ctttccttta tatcgcacga ttgtcaaccg
    20341 ggcttgatta caggcgcgga acagtgcatc ttttggtgcc acgagcccaa cgaagcacta
    20401 tttcagtgga gaacgaccgt gcgtcctgct actcctgcct gggagtcggc ttgttcgtgg
    20461 gcgtggtagc tggagcggac cagcggcccg gattccacat ggcggaatcc catggccagc
    20521 gcttcgcgtt tcatgaaagc gaattcatcg ggtgtgtagt agcgggcgat gggcagatga
    20581 tccttcgagg gccgcagata ctggccgatc gtcagcacgt cgacgccaac ggcagcgagg
    20641 tcgcggtaga cctggagcag ttcgtccatc tcttcgccga ggccgaccat gactcccgat
    20701 tttgtcaccg tcttcgcctc cacttctttc gcgaattgga gcaggcgcaa cgtgcgctca
    20761 tagcgagcgc cggaacgaac ggcgcggtag aggcgcggga cggattctgt gttgtggttg
    20821 aggatctcgg ggcgcgcgtc aacgacgagg cggattgcct cctcgttgcc ctggaagtcg
    20881 gggatgagga cttcgacctg gcagccgggc gcctggcggc ggatctcgtc gatgaccatc
    20941 acgaaggccc gagctccgcc gaccacgtcg tcgtcacgat tgacgctggt gatgacggcg
    21001 aacttcaggc cgagagcggc gacggcttcg gcgactcgcc gaggttcgtc gaagtcgatc
    21061 gggtcggggc ggcccttggg aacggcgcag aatccgcagc ggcgcgtgca ggtgttgcca
    21121 agcatcatga aagtcgcggt gcgatggtgc cagcactcgc cgatgttggg gcactgcgcc
    21181 gattcgcaga cggtgtgcag attgagcgag cgggcaagct tcttgaggtc gtgatagttg
    21241 tcgcccatgg gcgcgcgggc acggagccat tccggcttgg gcgatgggcg gcggggcgcc
    21301 agatcgatct ggatgagcgc ttcgcccggg atactcggat tgccagcaga gggagaggcc
    21361 atcgttgctt tgtctattgt agttcccaca agcggagatt cgccccgcac accgcaaagc
    21421 cggacttaag acttcgccgg cgtggcaggt tccgcgggag cggcttcctg gggcgggatc
    21481 agaggcagtt cttcaacgac cgcgagaccc gtgcggcgca ggagctgaaa gatggtgggg
    21541 ctggcgaggc gcgtggcatc gtactccacc cggattgtct tccactcttc atcgacctta
    21601 atgcggcgaa tcccgtaaac ctcgcgtaga ttgcccagtg cgaacatgat ggtttctgtc
    21661 ggatggcctt cgtatcggta gagcgcctcg agctgcgtca tgaatacagg gtacagggga
    21721 cggcaagcag ggtacagcgg tggagcaccg agttgaagcg gacaccaggt accagagttt
    21781 gcccaggttg gcctcagccc gctaccggca cccacacctg catcgagctc tcgtcccggt
    21841 tatcccaggc gtagtagggg atcagcttca gctccgccgc ttccacttgc gccggcgcaa
    21901 ccgccggcgc atacagtgac tcgacgggct cggcgcggcg agccccctcg tgggtcagca
    21961 ccatgacgcc gtccagcacg tccgccttgt actccccgcg gatcgcgccg ccacgcctgg
    22021 ccatgtagag agggaaattt ttcgcgtcgg tgcccttcgt ctggtcgagg ccctccatgc
    22081 agaagaccac cgggccgcgc tccatcgcga ccttgccggt gtcctgctcg acggctggat
    22141 tggcgaccac catgcgcggg gtcatgtcga agctgagctc gaccggggtt tcgccgctcc
    22201 accgccggcg aatcggcatg tactcgccgg gccgtacgcc ctcgaccggc ttgcccgcga
    22261 ccgccacgcg cgactcgcgc gaccacgccg ggacccgcac gtagagcgtg aactcctccg
    22321 gatgctcgac cgcaacggtc atccgcaccg cgccctccca cggatactgt gtcttctgcg
    22381 ttacgacgat ggggttgccg ctctcgagct tccactgcag gcgcgagttg tcgtagaggt
    22441 gaacgtacag gccatccttt gaggtcgagt agaagtagcc gggcaggctg gcaaaggtgc
    22501 gttcgagatt gggtgggcag caggtggtgt cgtaccaggg attgcgaatc ttttcgccgc
    22561 tggtgggatc gaaggccagc ggattgcggt agcaataaag agttcccttc agcgacatgc
    22621 cggagttgat gccgttatag agcgcgcgct ccatcacgtc ggtaaagcgc gcctcgccgg
    22681 tggcggccag catgcgccag ttccacatcg cgttgccgat ggccgcgcag ctttcgccgt
    22741 atgcctgggc attcggcagc tcgtaatcct cgccgaacgc ctcaccctca gcgcgcgcgc
    22801 ccacgccacc gttgatgtac atctgcgccc gcgtcaggtc gctccacagc acctcgagcg
    22861 tcttcatgta ggccgcgtcc ccggtttcca ggtagtaatc cgtcgcgccg cagttggcat
    22921 acatagcgcg caccgcgtgg ccctgcagtt tcgtcttcgt cgtgaaggga acgccgcagt
    22981 tcatgtagac gatctgctgc ggccgcagat tggcgcgcgg atcgccctgc aggatgtaac
    23041 cggccagatc gacgtatttg cgctcgcccg tggtgcgata gagctcgatc agggccattt
    23101 caatttccgg atgccctgag acgatcggct tcttgtccgg ctcgggcccg aagttgggga
    23161 gaatgtaatc gtccaccatc ttgcgtcccg cgttcatcag ggtggggtcg ccggtcgcac
    23221 ggtagtacgc aattgcgccc tgtagcatgt gtcccaggca gtagagttcg tgcccgcgca
    23281 cctgctcagg gatggtcatg cgcagagact tgcgatcgtc ctggtagtag gtgtcgaggt
    23341 agccattcgg ctcctgcacg gcaacgatct gctggatcgt cgtctgcgct gcttcgtgca
    23401 gttgcgggtt gtcctcggtc tgcagcgcga atgccacgcc ttcgagccac ttgtacatgt
    23461 cggaatccga atacacggga cccttttgcg gctcgttgga cttgcccacg agccggagga
    23521 agttgtccat gcgcccgtgg tcgagcagtt cctggtgcat cgaaggaata ctcgactcga
    23581 cgttggtctt ccgccgcttc gaccagaagc cttcctcgat ggtcacggca cgcaccggga
    23641 tgttgcgcag tttcgcatgc ggcgactgag cgagattcag gacgccctgg tcatgccagc
    23701 cgggggccgc gctcgtcacc ggctcatcga atccccacgc gcgcgtcgcc gccatgaggg
    23761 gaggcgccgc tgctgtggct ccggcgaggg aaagaaacgt tcttctggaa acggtatgtt
    23821 ttgccatggg aaacctcccg ggtcgtgata tctgctccgg cgcgccttca ttctagtccg
    23881 ggaaacgttt tctgcgctga cttttgcaac tcgcatccaa ttttccagtt gcgtcaatcc
    23941 atttacgtca atctatggac catcgtcgtt catttttcga tccccgctcc accggcatgg
    24001 tccgcgtccg cggcgctcgc gagcacaatc tcaaagacat ctccgtcgat atccctcgtg
    24061 acgccctcgt tgtcttcacg ggtgtctcgg gatcgggcaa gtcctcactc gcctttggca
    24121 ccctctatgc cgaggctcag cgccgctacc ttgaatccgt ctcgccctac gcgcgccgcc
    24181 tctttcatca acttaccgtt ccggaagtcg actctatcga cggcctcccg cccgcggtcg
    24241 ccctgcagca gcagcgcggc tcgcccacca cgcgatcgtc agtcggcagt gttactacgc
    24301 tctccaatct gatccggatg ctgtactcac gcgccggcga ctatccaccc aaagcgccga
    24361 tgctttatgc ggaaaacttc tcgcccaacc ggccggaagg cgcctgcccg aagtgccatg
    24421 gcctgggccg cattctgacc gtgaccgaga agtcgatggt gcccgatccg tcgctgacca
    24481 ttcgcgaacg cgccgtcgct gcatggccca cggcatggca gggacaaaat ctccgcgaca
    24541 tccttgtttc catgggctat gacgtcgacc gcccgtggcg cgatctgccg aagaaagacc
    24601 gcgactggat ccttttcacc gacgagcagc ccaccgttcc cgtctacgcc ggcttcacgc
    24661 ctgccgaaac ccgccgcgct ctgcgccaaa aaatggagcc cagctacatg ggcacgttta
    24721 cctcggcgcg gaagtatgtt ctcgatacct tttccaaatc ccaaagcgag cggatgaagc
    24781 gccgcgtctc cgagttcatg cttgccgccg agtgctccgt gtgccacggc aaacggctgc
    24841 gtgccgatgc gctggaggtt caattcgccg ggctcgacat cgccgcgctc agcgcgctcc
    24901 ccctgcgcca ggtccacgcg cgccttgcgc cctacgcctc tgccaaaaat tcgccgctgc
    24961 gcgtcacgcc cgaacgcgcc gccgtcgcac aacgcatcac gcaggacatc gtcgctcgcc
    25021 tcgacgcgct cctcctgctg ggtctcagct atctgtctct ggatcgttcc acgccgacgc
    25081 tctctccagg cgagctgcag cgcctgcgcc tggccacaca aatgcattcc aacctcttcg
    25141 gcgtcgtcta cgtcctcgat gagccgtctg cagggcttca tccctctgat acagaaaccc
    25201 tgctgcacgc cctcgacgcg cttaaacgtt ccggcaacag ccttttcatc atcgagcacg
    25261 aggtggatgt catccgccgg gctgactgga tcgtcgacgt cggccccggc gccggcgaca
    25321 agggtggcga gatcctctac agcggtccgc ccgccggact ccgcaatgtc gaggcctcca
    25381 aaacgcggct gtatctcgac gcccgaactc ccccgcgacg gacctcgcgt tcgccgtcgg
    25441 ggtggctgcg cctgcgcggc gccacgcgca acaatctcca caacctcgac gtcgattttc
    25501 cgcttggcgt cttcacgtcc gttaccgggg tttccggctc gggaaaatca acgctcgtca
    25561 gccaggtttt ggtcgaactc gcttctcgtc atctgggcca gagagtcgcc gacgaagaat
    25621 ccgccggcga ggatcgactc accgccgaga aaccaatcct caccggtggc tcgatcgagt
    25681 ccggtatgga aagcatccgc cgcctggtcg tcgtcgatca gaaacccatc ggacgcaccc
    25741 cgcgctccaa catggctacg tatacaggcc tcttcgatca cgtccgcgcc cttttcgcgt
    25801 ccaccagggt tgcccgcgaa cgccgctacg atgccggacg cttctccttc aatgtcgcca
    25861 aaggccgctg ccccacctgc tccggggaag gattcgtctg cgtcgagctg ctgttcctgc
    25921 ccagcgttta ctcgccctgc cc
//
LOCUS       DASZYN010000129         5726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_841151, whole genome shotgun sequence.
ACCESSION   DASZYN010000129 DASZYN010000000
VERSION     DASZYN010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841151"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VHE33_03400"
     CDS             <1..659
                     /locus_tag="VHE33_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96524.1"
                     /translation="SSTSQYPVRSDWVSVSDEDSVRQAAVTLNRYASLLARVHGWLQL
                     ADSPAGASNATYYSLALIPATGGSPIVSNTLLRQNDVLKIGLQANGRVREKRWVYILD
                     INCQGKGTVIYPGGNSDNQFPNESDTGSLFVLPGEPEIGISPPFGVDTLIMLSTAQPL
                     SDQYVLNFEGVARSRGAQSPLEQLLSNTSAGMRGDPAQLPTNWNIGFTTIRSVPLPNP
                     "
     gene            713..1174
                     /locus_tag="VHE33_03405"
     CDS             713..1174
                     /locus_tag="VHE33_03405"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 family protein"
                     /protein_id="HVT96525.1"
                     /translation="MLLLCAFLVGLVAGLRSMLAPAIVSWFARQNLLPVGGTYLALMS
                     YTITAIGFSLLAACELVVDKLPTTPSRKQPLSFAVRLLTGSLVGATVGATGNKIILGI
                     LCGAIGAVIGTLGGAAARAKLAALFGADYRAAILEDLVAIGIALFAILRVA"
     gene            1171..2547
                     /locus_tag="VHE33_03410"
     CDS             1171..2547
                     /locus_tag="VHE33_03410"
                     /inference="COORDINATES: protein motif:HMM:NF004992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HVT96526.1"
                     /translation="MKHSFDAIVVGAGQAGPSLATRLAGAGWQVAVVERHLFGGTCVN
                     TGCTPTKAMVASAHAAHLARRGADFGVHASSVTVDMRAVKARKDAIVSNARNGVEKSL
                     RTSKNCTVFTGTASFESRNVMRVGDDQIEASKIFLNVGARPTIPQMPGVDSVPFLTST
                     TILDLEDLPQHLIVVGAGYVGLEFAQMFRRFGSKVTVVDKSARLAPHKDEDASKVIRE
                     IFDSEEIAVRLNANCIHLERDDSNVAVGLECIDGEPRVVGSHILLAVGRTPNTHDLNL
                     AVAGITPDQHGYIPVDDFLQTTVPGIFAIGDCNGRGAFTHTSYNDYEIVADNLLDGAS
                     RRVSDRIPANALYIDPPLACVGMSEAEVRAIGKPALVGFRTMTRVSRAIEKGETFGYI
                     KVFVDAKSQQILGGSVFGTGGDEAVHCILTAMYARQPASLLTHSVHIHPTVAELIPTT
                     FEDLKPFP"
     gene            2544..3539
                     /gene="yiaK"
                     /locus_tag="VHE33_03415"
     CDS             2544..3539
                     /gene="yiaK"
                     /locus_tag="VHE33_03415"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF009750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HVT96527.1"
                     /translation="MTLRVPFDEVASRLSAILTRAGFETHRADLSARLFAETTRDGVY
                     SHGLERFPRLLAMIRNGSVDVHATPQRVQQFGAIERWDGRRGPGNLNAWTSMARALEL
                     SNQHGIGCVALANTNHWMRGGTYGWQAADAGAIGICWTNTLANLPPWGASLPAVGNNP
                     LIIAVPRPSGHVILDMAMSQFSYGALTSYQQKGKLLPVDGGFDSSGNLTRDPAAIEAS
                     QRPLPIGYWKGSGLAMMLDLIATLLSGGLATHQIPTDPLRESGLSQVFLALNLARLQP
                     AADSARTADGIVETVLASNPGAVRYPGEQILTTRSENLALGLPVREELWNELCAL"
     gene            3571..4572
                     /locus_tag="VHE33_03420"
     CDS             3571..4572
                     /locus_tag="VHE33_03420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HVT96528.1"
                     /translation="MMRAAVLHHPASIGSRPLALEDLPVPEPGPGQVLLKVEACGVCR
                     TDLHITAGELKPLNEAIVPGHQIVARVEKLGSGVDSIPIGKRAGVSWLGGVDGTCPLC
                     RKSRENLCDDPTYTGYSVNGGYAEYAIARADFLIPLAEDKPATDLAPLLCAGIIGFRS
                     LRVAEVSPGDRVGLFGFGASAHLAIAVLKSWNCEVYVSTRGASHQKLAHELGAFWVGD
                     ATERPPVPLDSAVTFAPSGDVVLAALASLRKGGIVAINAIHLDRVPQFDYDSLLWGER
                     QMRSVANMTRQDARDFVGLAAKIGIHPRTTVFRLDQVNDALAAVANDVIDGAAVVVP"
     gene            complement(4569..5270)
                     /locus_tag="VHE33_03425"
     CDS             complement(4569..5270)
                     /locus_tag="VHE33_03425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HVT96529.1"
                     /translation="MALKYAGNTKRKPEETWVAVEGYLDRLQIAPDAALEAALADSAK
                     AGLPEIAVTPSQGKLLHLLAKMQGARRILEIGTLGGYSTIWLGRALPARGRLVTLEAE
                     KKHAEVARRNIARAGLSGKVEVRLGPALRTLSQMTTERKPSFNLVFIDADKESTAEYF
                     DYALRLSRAGSVIVVDNVIRDGEVANVDTDDPRAQGIRRFNEAVAREKRVSATTIQTV
                     GSKGYDGFTLVLVLR"
     gene            complement(5283..>5726)
                     /locus_tag="VHE33_03430"
     CDS             complement(5283..>5726)
                     /locus_tag="VHE33_03430"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HVT96530.1"
                     /translation="VRSDNMRDAIARAEALTEVRGSEDLAAIAAAFKRIKNILRQAQE
                     KGVLNPGSQFGFMSRDLLHDPAELALYEESMKLAPVVEELGRHGEYRQAMEKIATLRP
                     HVDLFFDKVMVMVDDKSLRDTRLQLIGTVLQAFSRIADFSELVTT"
BASE COUNT         1048 a         2043 c         1465 g         1170 t
ORIGIN      
        1 gctcctccac ctcgcaatat cccgtccgca gcgattgggt ttccgtatcc gacgaagatt
       61 cagtgcggca agcggccgta accctcaacc gttacgcttc gcttctcgcc agggtgcacg
      121 gctggctcca gctcgccgat agccccgccg gcgcatcgaa cgcgacatac tactcactgg
      181 cgctgatacc agctacagga ggctcgccca tcgttagcaa cacgctgctc cgccagaatg
      241 atgtcctgaa gatcggtctc caggctaacg ggcgcgtgag agaaaagcgt tgggtctaca
      301 tcctcgacat caattgccag ggtaagggca ctgtcattta ccctggagga aattcagaca
      361 atcaatttcc caacgaatcc gacaccggca gcctcttcgt tttgcccggc gaaccggaga
      421 tcggcatcag tccgccattt ggagtcgata cgctgatcat gctcagcacc gcccagccac
      481 tgtccgatca gtacgtcctg aacttcgaag gggtcgcaag gtcccgcggc gcccaatcgc
      541 cgctcgaaca actgctgagt aatacaagcg ccggaatgcg cggcgatccc gcacaattac
      601 ccacaaactg gaacatcggc ttcaccacaa ttcgcagcgt tccgctaccc aacccttaac
      661 cctggaccac cagaacaacc cgatccactt gctgtacttg aggagatttt ccatgcttct
      721 gctttgtgcg tttctcgttg gcctcgtcgc cggcctgcgc tccatgctgg ctcccgcgat
      781 cgtcagttgg tttgcccgtc agaacctgct ccccgtgggg ggcacctacc tcgcgctgat
      841 gagctatacc atcactgcga ttggttttag tctgctcgct gcttgcgaac tcgtcgtcga
      901 caaactgccg accaccccca gccgcaaaca gcccctctcc ttcgccgttc gcctcctcac
      961 cggctctctc gtgggcgcca ccgtcggtgc aaccggaaac aaaatcatcc tcggcatcct
     1021 ctgcggcgcc atcggtgcag tgattggcac cctcggcggc gccgccgctc gagccaaact
     1081 cgcggcgctc ttcggggctg actaccgtgc cgcgattctc gaagatcttg ttgcaatcgg
     1141 aatcgctctc tttgccatcc taagggttgc atgaaacatt cctttgacgc gattgtcgtc
     1201 ggtgccggcc aggcgggtcc ctcgcttgcc actcgactcg ccggcgccgg ttggcaggtc
     1261 gccgtggttg agcgccatct ctttggtgga acctgcgtca acaccggatg cacccccacc
     1321 aaagccatgg ttgccagtgc acacgctgct cacctcgcgc gccgcggtgc cgatttcggc
     1381 gtccacgcaa gctcagtcac cgtcgacatg cgcgccgtca aagcccgcaa ggacgcaatc
     1441 gtctccaacg cgcgcaacgg cgttgaaaag tcgctgcgta catcaaagaa ctgcaccgtc
     1501 ttcaccggca ctgcctcgtt cgagtcgcga aatgtcatgc gcgtcggcga cgaccagatc
     1561 gaagcatcga aaatcttcct caacgtcggc gctcgcccca ccatccccca aatgcctggt
     1621 gtcgattccg ttccgttcct caccagtacc accatcctcg atctggaaga ccttccgcag
     1681 cacctcattg tcgtgggtgc tggttacgtc ggcctcgaat ttgcgcaaat gttccgccgc
     1741 ttcggctcta aggtcacggt cgtcgataag agcgctcgcc tcgctcccca taaggacgag
     1801 gatgcctcga aagtcatccg cgaaatattc gactccgaag agatcgcagt tcgcctcaat
     1861 gccaactgca tccatctcga acgcgatgac tcaaacgtag ccgtcggcct tgagtgcatt
     1921 gacggagaac ctcgcgtcgt cggctcccat atcctcctgg ctgtgggtcg tacgccgaac
     1981 acccacgacc tcaatctcgc tgtcgccggt atcaccccgg accagcacgg ttacattccg
     2041 gtcgacgact tcctgcagac caccgttcct ggcatcttcg cgataggcga ctgcaacggt
     2101 cgcggcgcct ttacccacac gtcgtacaac gactacgaaa tcgtcgccga caatctgctc
     2161 gacggcgcat cccgtcgcgt cagcgatcgc attccggcaa acgccctcta catcgatccg
     2221 ccgctcgcct gtgttggtat gtccgaagcc gaggttcggg ccatcggcaa gcctgcgctc
     2281 gtcggcttcc gcaccatgac ccgggtcagt cgcgcaatcg aaaagggcga aactttcgga
     2341 tacatcaagg tcttcgtcga tgcaaagtca cagcaaattc tgggaggatc tgtcttcggt
     2401 acaggcggcg atgaagccgt ccactgtatc ctcacagcga tgtacgcgcg ccagcccgca
     2461 tcgctcctca cgcattcggt ccacatccat cccacagtcg ccgaactcat tcccactaca
     2521 ttcgaagacc tgaagccttt tccatgaccc tccgcgttcc tttcgacgaa gttgcatcca
     2581 gactgtccgc tatcctgacc cgggcgggct ttgagactca ccgcgccgat ctcagcgccc
     2641 gcctatttgc cgaaaccacg cgcgacggtg tctattcgca cgggttggag cgctttccgc
     2701 gcctgctagc catgatccgc aacggctcgg ttgacgttca cgccacgcct caacgggtgc
     2761 agcaattcgg tgccatcgag cgctgggatg gccgccgcgg tcctggcaat ctcaatgcgt
     2821 ggacctccat ggcacgcgcc ctcgaactca gcaaccaaca tggcatcggc tgtgtcgccc
     2881 tcgccaatac aaaccactgg atgcgcggcg gcacctacgg ctggcaggcc gccgatgccg
     2941 gcgccatcgg catatgctgg acaaacaccc tcgccaatct tcccccgtgg ggcgcttccc
     3001 tgcccgcggt cggcaacaat ccgctcatca tcgctgtgcc ccgcccctcc ggccacgtca
     3061 ttctcgatat ggccatgtcg cagttctcct atggcgcgct taccagctat cagcagaaag
     3121 gcaagttgct tcccgtcgac ggcggctttg actccagcgg caatctcacg cgcgatcccg
     3181 ccgccatcga agcatctcag cgccctctcc ccatcggcta ttggaaaggc tcaggactcg
     3241 ccatgatgct cgacctgatc gcgaccctgc tctccggcgg tctcgctacg catcagatcc
     3301 ccaccgatcc cctgcgcgaa tccggcctct cccaggtctt ccttgcactg aatctcgcgc
     3361 gtctgcagcc cgccgcagac tccgcccgta ccgcagatgg cattgttgaa acggttctcg
     3421 ccagtaaccc aggagccgtc cgctatccgg gcgaacagat cctgaccacc cgctccgaga
     3481 atctcgctct tggcttgccc gtccgcgaag aactctggaa cgaactctgc gcactctgat
     3541 gcggagcccg gcacggcgct aatctaaata atgatgcgcg ccgccgttct gcaccatccc
     3601 gcttccatcg gttcgcgccc gctcgcgctc gaagaccttc ctgtccccga gccgggcccc
     3661 ggacaggtct tgctcaaagt cgaagcttgc ggcgtctgcc gcacggacct ccacatcacc
     3721 gcaggcgaac tcaagcccct gaacgaagcg atcgtccccg gccatcagat tgtggcgcga
     3781 gtcgaaaagc ttggctccgg tgtcgactct atccccatcg gtaaacgcgc cggggtgtcc
     3841 tggctcggcg gggtcgacgg cacctgcccg ctttgccgca agagtcgtga gaacctctgc
     3901 gacgatccca cctacaccgg ctactccgtc aacggcggct acgccgaata tgccatcgcc
     3961 cgtgccgact tcctgatccc gctcgctgaa gacaagcccg cgacggacct cgctccgctg
     4021 ctctgtgcag gcattatcgg atttcgtagt ctgcgcgtcg ccgaggtttc ccctggcgat
     4081 cgcgtcggcc ttttcggctt cggagcgtcg gcgcatcttg ccatcgccgt cctcaaatcc
     4141 tggaactgcg aagtctatgt ctcgacgcgc ggcgcctctc accagaagct cgctcacgaa
     4201 ctcggcgcct tctgggtcgg cgacgccacc gagcgcccgc ctgttccgct cgactcagcc
     4261 gtcaccttcg cccccagcgg cgacgtcgtc ctcgccgcac tcgcctccct gcgcaaaggc
     4321 ggcattgtcg ccatcaatgc aatccacctc gatcgcgttc cacagttcga ttatgattcc
     4381 ctgctctggg gcgaacggca gatgcgcagc gtcgccaaca tgacccgtca ggatgcccgc
     4441 gacttcgtcg gtctcgcagc aaagatcggc attcacccgc gcacaaccgt cttccgcctc
     4501 gatcaggtga atgatgcgct ggcagccgtc gccaacgacg ttatcgacgg cgctgcggtt
     4561 gtggttcctt agcgaaggac aagcaccagc gtaaatccgt catagccctt gctcccaacg
     4621 gtttggatcg ttgttgcgct gactcgcttc tcccgcgcca cagcttcatt gaaccgccgg
     4681 attccctgcg ctcgcggatc atccgtatcg acgttggcca cctcgccatc gcgaatcacg
     4741 ttgtccacca caatcaccga gcccgcacgc gatagtcgca gagcatagtc gaaatattcg
     4801 gcggtgctct ccttatcggc gtcgatgaaa acaagattga acgacggctt tcgttccgtg
     4861 gtcatctgcg acagtgttct gagcgcgggc ccgagccgca cctccacctt gccgctcaac
     4921 ccggcccgcg cgatattcct gcgcgcaacc tcggcatgtt tcttctccgc ctccagcgtc
     4981 accagccgcc ctcgggccgg caatgcgcgt ccgagccaga tcgtgctgta tccgcccagc
     5041 gttccaatct ccagaatccg ccgcgccccc tgcatcttcg ccaacaggtg cagtagtttt
     5101 ccctgactgg gggtcactgc aatttcgggc agccctgcct ttgcactatc tgcaagagca
     5161 gcctccagag ctgcatccgg cgcaatctgc agcctgtcca gatagccctc gacagcaacc
     5221 caggtctcct caggttttcg cttcgtgttt ccggcgtact tcaatgccat tcgctcgtct
     5281 cgctacgttg tcaccagttc ggagaaatcc gcgatccgcg aaaacgcctg gagcaccgtt
     5341 ccgattagct gcagccgggt atcgcgcagt gatttgtcgt ccaccatcac catcaccttg
     5401 tcgaagaaca aatccacatg tggccgcagc gtcgcgatct tctccatcgc ctgccggtat
     5461 tcaccgtgcc tgcccaactc ctcaacgaca ggcgccagct tcatcgactc ctcgtacagt
     5521 gccagctccg ctggatcgtg cagcagatct cggctcatga agccaaactg actgccggga
     5581 ttcagaacgc ccttctcctg cgcctgccgc aggatattct tgatccgctt gaatgctgcc
     5641 gcgatcgctg ccaggtcttc cgaaccccgt acctcggtca acgcctcggc ccgtgcgatc
     5701 gcatcgcgca tgttatccga ccgcac
//
LOCUS       DASZYN010000130         4234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_69933, whole genome shotgun sequence.
ACCESSION   DASZYN010000130 DASZYN010000000
VERSION     DASZYN010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69933"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1054)
                     /locus_tag="VHE33_03435"
     CDS             complement(89..1054)
                     /locus_tag="VHE33_03435"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HVT96531.1"
                     /translation="MLKVGVPEAVDPKLLGLLPDGISLERIPIQPSEPIEVEFWVPPP
                     WQGQAGRQLPFLRGLKVIQSALAGVDQLLKIKPPGVVLCDARGAHSISTAEWAVAAVL
                     ASNKYFPLFGQVQRAGLWSRRKEAEDLYHTLHPGTHRASPPILCEELHGREVLIVGYG
                     SIGQAIEERLRPFGVTFVRVARTAREGVHGVSELAGLLPSADVVILIVPFTQETVGMM
                     GGKEIGLMKQGAMLVNAARGPVVDTDALLAALHAGRITAAIDVTDPEPLPDGHPLWTA
                     PNLLITPHVAASTPMFMVRAMQFAGVQMGRYLRGEPLENVVTGEY"
     gene            complement(1306..2484)
                     /locus_tag="VHE33_03440"
     CDS             complement(1306..2484)
                     /locus_tag="VHE33_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVT96532.1"
                     /translation="MTTRNKPIRLAKRLDEVGFSDIVQIRNKVIELRAAGKLVHAFHG
                     GEPFFETPDTVKYANLKALVENKTRYAPSSGIEPLRAAIARKLQAKNHIDASVEDVIV
                     TCGGAHALHVAFEAVLDAGDDVLVFSPFWTPIKDMVTLAQARPVMVPTATARRNGLTA
                     TLEHFSTPATRAIYYNTPQNPSGVVFTRSEAEEVAAFARKHHLIVIADEAYEDLVYDG
                     EHFSIASLPGMAERTITAYTFSKTYAMTGWRAGYAVAKEPFMTGLRKLVLYSTNGVST
                     PTQWAALEAMAIQEATIAARREEYRQRRDLLVSGLCELGLECEVPAGAFYAFPSVRKI
                     DKDSRKVAALLLDKAHVATIPGVVFGAQGEGHVRFSYAVPPEAIETGLEALGKVLRQA
                     "
     gene            complement(2578..3252)
                     /locus_tag="VHE33_03445"
     CDS             complement(2578..3252)
                     /locus_tag="VHE33_03445"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HVT96533.1"
                     /translation="MLIPSIDLMGGRIVQLERGEELRLAFDNFEYWIERFSRFPLVQL
                     IDLDAAKRTGDNRALIAQIARRLPVQVGGGIRTIDQAQALLNGGARRVIFGSALFSDN
                     GINAEFAAGVSESIGPSRFVAAIDTKAGKIAVRGWKDNVDLTPAQAIAALDPWCGAYL
                     YTHVDTEGTMGGFPIEAAKALRPLTSRQLIVAGGIRAQAEIDALDAMGVDAVAGMAVY
                     SGHLIT"
     gene            complement(3246..4019)
                     /gene="hisF"
                     /locus_tag="VHE33_03450"
     CDS             complement(3246..4019)
                     /gene="hisF"
                     /locus_tag="VHE33_03450"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HVT96534.1"
                     /translation="MLTKRIIACLDVRDGRVVKGIQFVDIVDAGDPAGLAARHAASGA
                     DEIVFLDITATHEGRGTLIDAVRRAARQLFVPFTVGGGIRSADDAAAIFDAGADKISI
                     NSAALARPELIGEIGAKFGAQAVVVAIDARRIPDRPVDEAEVFVSGGRKPTGRRAVEW
                     AREAESRGAGEILLTSMDSDGTRAGFDCELTAAVSEAVQIPVIASGGAGNAQHFTQVF
                     REGKADAALAASIFHFGIHDVRALKEELQRDGIPVRLPC"
     gene            complement(4013..>4234)
                     /locus_tag="VHE33_03455"
     CDS             complement(4013..>4234)
                     /locus_tag="VHE33_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96535.1"
                     /translation="SKLLAGISNNSFAYFTHSYRAPVTNVTVAITDYGEPFAAAVERD
                     NVMGVQFHPEKSGETGLRVLRNFLEWRPC"
BASE COUNT          817 a         1373 c         1274 g          770 t
ORIGIN      
        1 ctgggccttt aagctaaccg ctctttagct aaccggatct tagccgaccg gcctttagct
       61 gaccggctct tagctccaca ccgctccatc agtactcgcc tgtcaccacg ttctcgagcg
      121 gctcgccccg cagataccgc cccatctgca ccccggcaaa ctgcatcgcc cgaaccatga
      181 acatcggcgt cgacgcagcc acatgcggcg tgatcagcag attcggtgcc gtccacagcg
      241 gatgcccgtc cggcaaaggc tccggatcgg tcacatcgat cgctgccgtg atgcgccccg
      301 cgtggagcgc agccagcaac gcatcggtat cgacgaccgg cccgcgcgcc gcattcacca
      361 gcatggctcc ctgcttcatc aggccgatct ccttcccgcc catcatccca accgtctcct
      421 gggtgaaagg cacgatcagg atcacgacgt ccgccgaagg cagtagcccc gccagttcgc
      481 tgaccccatg aacaccctcg cgtgcggttc gcgcaacgcg aacaaaagta acgccgaacg
      541 gccgcaggcg ctcctcgatc gcctggccaa tcgagccgta tcccacgatg aggacctctc
      601 gcccgtgaag ttcctcgcaa agaatgggag gagaagcccg atgcgtgcca gggtgaagcg
      661 tgtggtaaag gtcctcggcc tctttccggc gcgaccacag gcccgcccgc tgcacctgtc
      721 cgaacagcgg gaaatacttg ttcgaagcca gcaccgccgc gaccgcccac tccgctgtcg
      781 aaatgctgtg ggcgccacgc gcatcgcaaa gaacgacgcc aggaggcttg atcttcagca
      841 gctgatcgac gcccgccagc gccgactgaa tcaccttcag cccgcgcaga aacggcagct
      901 gccgcccggc ctgaccctgc cacggcgggg gaacccagaa ctcaacctca atcggttcgc
      961 tcggctggat cgggattcgc tccagcgaaa ttccatcggg taacaggccg agaagtttcg
     1021 gatcgacagc ttcgggcacg ccaactttga gcatcgaacg cagctccgtt tcgcgagact
     1081 catatcgttc aggctcgcga agtcttcttc gatgctagtg gggtgtcctg gaagtaagca
     1141 aagtaaattt gtctcccgac cggactctga gcgtagcgaa ggggaagcgg agggacctgc
     1201 tttttcttcc atttcagacg tcaccaatca ctggagcaag caactgggtc gagcgcgatg
     1261 tatcgatacg ccgcgaggcg gcctccggcc gctctttgct ggttgctatg cctggcgcag
     1321 gaccttgccc agggcttcga gtccggtctc gatcgcctcc ggcggcaccg cgtagctgaa
     1381 acgaacgtga ccctcgccct gcgccccgaa gacaacgccg ggaatcgtcg caacgtgcgc
     1441 cttgtccagc agcagcgcgg cgaccttacg gctgtcctta tcgatcttgc gaacgctcgg
     1501 gaatgcgtag aaggcccccg cgggaacctc gcactccagc ccaagttcgc acagcccgct
     1561 gaccagcaga tcgcgccgct ggcgatattc ctcgcgtcgg gcagcgatcg tcgcttcctg
     1621 gatcgccatc gcttccagcg ccgcccactg cgtaggcgtc gatacgccgt tggtcgagta
     1681 gagcaccagc ttgcgcagac cggtcatgaa tggctcttta gccaccgcat aacccgcgcg
     1741 ccaaccggtc atcgcatagg tcttcgagaa ggtgtaggcc gtaatcgtgc gttcggccat
     1801 gcccggcagg gatgcgatgg agaagtgctc gccatcgtag acgaggtcct cgtacgcctc
     1861 gtcggcaatc acgatcaggt gatgcttcct ggcgaacgcg gcaacctcct ccgcttcgct
     1921 gcgcgtgaag accacgccgg agggattctg cggcgtgttg tagtagatcg cccgcgtcgc
     1981 cggtgttgag aagtgctcga gcgtcgcggt caggccgttg cgccttgctg tggccgtggg
     2041 aaccatcacc ggccgcgcct gggcaagcgt caccatatct ttgatcggag tccagaaagg
     2101 agaaaaaacc agaacgtcat cgccggcgtc gagcactgcc tcaaaggcca cgtgcagagc
     2161 atgggctccg ccgcaggtca cgatgacatc ttcgaccgac gcgtcgatgt ggttcttcgc
     2221 ctgtagcttc cttgctatgg cagcccgcag cggttcgatt cccgacgaag gtgcatagcg
     2281 cgtcttgttc tccaccagcg ccttcaggtt ggcgtacttg accgtgtcgg gagtctcgaa
     2341 gaaaggctcg cccccgtgga acgcgtggac cagcttcccc gcggcccgga gttcaatcac
     2401 cttgttgcgg atctgcacga tgtcggagaa cccgacttca tcgaggcgct tcgcgaggcg
     2461 gattggtttg ttcctggtcg tcatcttggg ttcacccacc ggacgggacg tttcagctta
     2521 gagcattttg atggaaggaa ggcgaaaatt gttcgcaggt cgcatccagc ccgaacttca
     2581 cgtgatcaga tgcccggaat aaacggccat cccggcgacg gcatccacac ccatagcatc
     2641 gagcgcatcg atctccgcct gcgcacgaat gccgcccgca acgatcagct ggcgcgaggt
     2701 gagcgggcgc agtgctttcg cggcttcgat ggggaagccg cccatcgtgc cttccgtatc
     2761 cacgtgggta tagagatagg ctccgcacca gggatcgagc gcggcgatcg cctgagccgg
     2821 cgtcaggtca acattgtctt tccacccgcg aacggcaatc ttgcccgcct ttgtatcaat
     2881 ggctgcgaca aatcgcgagg ggccaatgct ctccgagacg cctgcggcga actccgcatt
     2941 gatgccattg tcgctgaaca atgccgagcc aaaaataacg cggcgggcgc ccccgttgag
     3001 gagcgcttgc gcctgatcga tcgtgcgaat ccctcccccc acctgtacag gcaggcgcct
     3061 cgcaatctgc gcaatgagag ctcgattgtc gcccgttcgt ttcgcagcat cgagatcgat
     3121 gagctggacc aaaggaaacc gcgagaaccg ctcgatccag tactcgaaat tgtcgaaggc
     3181 gagtcggagc tcttcgccgc gttcgagctg cacaatgcgc ccgcccatca ggtcgattga
     3241 aggaatcagc atggaagcct caccggaatg ccatcgcgct gcagctcttc tttcagcgcg
     3301 cggacatcgt ggatgccgaa atggaagatg ctggcggcga gagcggcatc ggccttgcct
     3361 tcgcggaaaa cctgcgtgaa atgctgtgcg ttgcccgctc cgccggatgc gatcaccggg
     3421 atttggacgg cttcgctgac cgcggctgtc agttcgcagt cgaaaccggc gcgagttccg
     3481 tcagaatcca tcgaggtcag gaggatctct ccggcgccgc gggactcggc ctcgcgcgcc
     3541 cactcgaccg cgcgacgccc ggtgggcttg cgtcccccgg agacgaagac ttcggcttcg
     3601 tcgaccgggc ggtcgggaat acgccgcgca tcgatggcga cgacgaccgc ctgggcgcca
     3661 aacttcgcgc cgatttcacc gatcagctcc ggacgagcca gcgccgccga gttgatgctg
     3721 atcttgtcgg cgccggcatc gaagatagcg gccgcgtcgt cagcggagcg gataccgcca
     3781 ccgacggtaa aagggacgaa gagctgccgg gcggcgcgac gaaccgcgtc aatcagggtg
     3841 ccgcggcctt cgtgcgtggc agtgatgtcg agaaagacga tctcgtcggc tccggacgcg
     3901 gcgtgacgcg cagcgagtcc ggcgggatcg ccggcgtcga cgatgtcaac gaactggata
     3961 cctttcacga cgcgtccgtc gcgcacgtcg aggcaggcga tgatccgctt agtcagcatg
     4021 gacgccactc caggaaatta cgcaggacgc gaaggccggt ttccccggat ttctcggggt
     4081 ggaactggac acccatcaca ttgtcacgct cgacggccgc agcgaaaggc tcaccgtagt
     4141 cggtaatagc tacggtgaca tttgtgacgg gcgcgcggta agaatgggtg aaataagcga
     4201 agctgttgtt actgattcct gccaaaagct tcga
//
LOCUS       DASZYN010000131         6680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1033509, whole genome shotgun sequence.
ACCESSION   DASZYN010000131 DASZYN010000000
VERSION     DASZYN010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033509"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="VHE33_03460"
     CDS             complement(<1..853)
                     /locus_tag="VHE33_03460"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT96536.1"
                     /translation="MARTRRISVVSLCVICAMVVIVAWSTPTFGQPFTSVTTSQQRMS
                     TGRLSIDQEIQLGNDYMVGHGVSKDEKQAAYWYEKAAEAGEPSAQKQIGFFYQAGIGV
                     AADPVRAVHWYQLAAANGLVTARTNLGVAYLWGSGVRKDLSMAAQLFRQAAEKGDGRA
                     ATYLGNMYFAGNGMQQDKAAAEHWYRVGAKQHDPVAEFDLGALFSVEDHPHDFEKAAK
                     WYRKSLEGGYVPAMHSLALLLEDHPELAKSDREFLHLFEQAAGLGQWKSSLALGMLFN
                     EGKLVPRD"
     gene            1316..2116
                     /locus_tag="VHE33_03465"
     CDS             1316..2116
                     /locus_tag="VHE33_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HVT96537.1"
                     /translation="MLQALGGSMILQAVLADDEPLARLKLSQMLAEIQGVEVVGEAAS
                     AAEVLDLVRETTPQLLFLDICMPGVDGFALVDQLNALTGIALPRIIFTTAYDTYALRA
                     FDMNAVDYLLKPFTIDRLRTAVDRARDEIRNAGKTVGQHEGAGQNGGRYTRRIVFKSR
                     GRILFLPVSDIRWIGAEENYVRICTKTETHLLRQTMTDLEGKLDPEMFARVHRSAIVN
                     LQYVKEVRTESRRDFAVLLVNGQKIPMSRSYHARILQSFKAARPSQVA"
     gene            complement(2175..2894)
                     /locus_tag="VHE33_03470"
     CDS             complement(2175..2894)
                     /locus_tag="VHE33_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT96538.1"
                     /translation="MAAQFRGKVALIAGGTGGLGRAVSLAFLRASARVIVTWRRQEEF
                     AALANDAADHRDLLTGLELDVTDEAATQQRLGGVIGDMGGIDILVNAVGGYAGGTPLW
                     SISPEVWDRMMALNLRSGYSLCRAVVPLMLNRARGRIVNVASRAAFDHAAGAAAYAAS
                     KAAAVAMMDSLAADLRGKGIRVNSVIPSIIDTEANRRAMPNSDFTKWPKPEEIANVIL
                     FLCSDDAKLIHGASIPVYGDS"
     gene            3249..6644
                     /locus_tag="VHE33_03475"
     CDS             3249..6644
                     /locus_tag="VHE33_03475"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT96539.1"
                     /translation="MVARSFSRIFAVLCVMGCLTCCSGAFAQAITGTINGSVHDASGA
                     AITGAAVKIQDTVHNVQARAVQTDANGNYTAPQLQPGMYAITATAQGFKSASVLDVHL
                     NVGDTLAINLTLPVGSSTETVTVSASDVQVNTQSASESTLINGAQMVGLPLNNRNYEQ
                     MVGLQPGVAYGGGDQLYIGLSNPSGETNVVAFSINGQRSSANTWTVDGADNVDRGSNY
                     TLLIYPSIDAISEFRTIRNTYSAEFGRSASGQINLITKSGTNLLHGDLHEFNRGDYDA
                     ANTYSNKLSGLARPPLHYNNYGYTIGGPVWLGKLYDGRNKTFFFFSQEFRRVITYSSL
                     TLAGDPTAAMRSGTFPVPVCVAVDANGKCTQTSTQINNIDPTAQAYIKDVFSKIPAGN
                     IPDSSTNANITLNTIERNLYNETQEIGRLDQNFGNKFSVFFRVVDDSIPTVEPGGLFQ
                     GAGFPGVGVTSTNAPGRNYLGHFLYTFSPKFLVDGGFAYSYGAILSDPIGYLDSNQSP
                     DVKPTLPYVSTLGRVPSLTFTGGTALGSYGPYRDYNRDRNPYANLTYILGKHSLRAGA
                     SYHYYQKSENAAGSNAGSFGFTNTGAPSGTGSYYQSWAWFLLGQQASFSQASLDLTPD
                     IRMRQFEAYLQDDWKVTPRLTINAGVRYSYFQQPTDAHKMLTTFDPLIYDSSKAPTID
                     STGHICKTAPCAGGAAPNAGYDPTNGIVVAGTSGAPFGDHPAPQMNKAFAPRVGFAYD
                     LFGNGKTAIRGGYGIAFDSSLVGDIEQNIFQNPPYVQNISINAGSLSNPLSGTVNVST
                     VPPALHATQYQSALPYGQQFSLDLQHQWAPDLISDIGYVGSLGTHLLGEVDLNQPRPG
                     AWQSLGVPSITRSNTTLLNRIRPWQGYDAINAIEPWFSSNYHSLQAAVQKRFKDGSLI
                     DANYTWSKALTNNQTDRSTAVQNLYNIAGEYGPAQYDIRHMFNADFVYALPFYRTQQG
                     VIGHVLGGWQLGGLVYMNGGVPLTVTTSADPAGLGLLDSASAAGARPNQTGNPNSGSG
                     IHTRTNWFNKSVFVADTNPNLPGNEHRGSVRGPGWQRWDLALYKNINIGERVKFQFRG
                     ETFNTFNHTNYSGVTVSNTSSLFGYVTSYRDPRIIQLGGKLNF"
BASE COUNT         1377 a         2185 c         1788 g         1330 t
ORIGIN      
        1 gatcgcgcgg gacgagcttt ccttcattga aaagcatgcc gagcgcgaga gaggatttcc
       61 actgaccgag accggcagcc tgctcgaaca gatgcagaaa ttcgcgatcc gatttcgcca
      121 gctcaggatg atcctcaaga agcaaagcga gcgagtgcat cgcgggaaca taccctccct
      181 ccagggattt tcggtaccat ttcgccgcct tctcgaaatc atgaggatga tcctcaacgg
      241 agaacagcgc tcccagatcg aactcggcaa caggatcatg ctgcttcgca cccaccctgt
      301 accagtgctc tgcggcggcc ttgtcctgct gcattccgtt tcccgcgaaa tacatatttc
      361 caagataggt ggctgctctc ccgtcgccct tttccgcagc ttgccggaag agttgggccg
      421 ccatggataa atccttacga acaccgcttc cccacaggta tgccactccc aggttggtgc
      481 gggccgtgac caggccattc gccgccgcca actggtacca gtgcactgcg cgcaccggat
      541 ccgcagccac accaatacct gcctgataga agaatccgat ctgtttctgc gcggagggct
      601 cgccggcttc cgccgccttt tcgtaccagt acgctgcctg cttttcgtct ttgctcacac
      661 cgtggccaac catgtagtcg ttgccgagct ggatttcctg gtcgatggag agcctcccgg
      721 tgctcattcg ctgctgactg gttgtgacgc tcgtaaacgg ctgaccgaag gtgggcgtac
      781 tccatgccac aatgacaacc attgcgcata ttacgcagag ggaaacgacg gagatccgac
      841 gcgtgcgtgc catggctttt ctcctgcact gggggaggca gcagctgacc tggatgaatt
      901 gctgatgccc cctcagacac gcgccattac gtgcagggta gcggccatgt tcgacgctgc
      961 cggttgcatt cgtcccgtct ttctgaggac agaaactcac tgctggccga gctgatcccg
     1021 acaaccactc tgttcgtcac aaatgcatcc atagccggtt ttgtgacccg ttggaaaacg
     1081 gtcatgaccg gtgggcgcaa cctgtcgact cgttgcgcgt tgtgaacccc aggtcactgt
     1141 tcgatcccgg acaactcagt ccggttccgg gcacccggga aggtcgcacc acctgtgagg
     1201 caatgtcgag cacacatatt tttcagcagg atgcagccga tggccgtttg gtcacgaacg
     1261 ggcatgaaac gtcccacaag tcctggtgtg gggagctgac ttccaggttc tccaaatgct
     1321 ccaggctttg ggaggctcga tgattctcca ggcggtactg gccgatgacg aaccactggc
     1381 gcgactgaaa ctcagccaaa tgctcgcaga gatccagggc gtggaggtcg ttggagaggc
     1441 tgcttcagca gctgaggtcc tcgatcttgt gcgcgaaacc acaccgcaac tgctttttct
     1501 ggacatctgc atgcccggcg tagatggctt tgcactggtt gatcaactca atgctctgac
     1561 cggcatcgcc ttgccacgca ttatcttcac aaccgcttac gacacctatg cccttcgggc
     1621 gttcgacatg aatgctgtgg actacctcct taagccattc acgatcgacc ggctgcggac
     1681 ggcggtggac cgggcccgcg atgaaatccg gaatgccggc aagacggtgg gccagcacga
     1741 gggagccggc cagaatggcg gccgttacac gcggcgcatc gttttcaaat cgcgcgggcg
     1801 aatcctgttt ctgcctgtct cagatattcg ctggatcggc gcggaagaga attacgtccg
     1861 catctgtacg aaaacggaaa cccatttgct gcggcagacg atgaccgatc ttgaaggcaa
     1921 actggatccg gagatgttcg cgcgcgtgca tcgttcggcc attgtgaatc tgcagtacgt
     1981 aaaagaggtg cggacggaat ctcgtcgcga cttcgccgtc ctgctcgtca acggccagaa
     2041 gatccccatg agccgcagct atcatgcgcg gattctccag tcctttaagg cggcacgccc
     2101 ctcgcaggta gcctgagcct caggcaaatc aggatgcccg tcggcgaaat cgccggcggg
     2161 tattcttttt gggcttagct atcgccgtaa accgggatcg aggctccgtg gatcagcttc
     2221 gcgtcatcgc tgcacaggaa caggatcacg ttcgcgatct cctcgggctt tggccacttc
     2281 gtgaaatcag aattcggcat cgccctgcgg ttcgcttctg tgtcgatgat gctcggaata
     2341 acggaattga cacgaatccc cttgccccgg agatcggcgg ccagtgaatc catcattgcc
     2401 actgcggctg ctttcgatgc ggcataggct gctgcccccg ccgcgtggtc gaaggcggcg
     2461 cgcgacgcca cattcacgat acggcccctg gctctgttta gcatgagtgg aactacggct
     2521 cggcataagg aatagccgga gcgcagattg agcgccatca tccggtccca cacctcgggc
     2581 gaaatcgacc agagcggcgt cccgccggcg taaccgccga cggcattcac cagaatatcg
     2641 atgccaccca tgtcaccgat cacgccgccg aggcgctgtt gcgtggccgc ctcgtccgtc
     2701 acgtccagct ccaaacccgt gagtaagtcc cggtggtctg ccgcatcgtt cgccaatgcg
     2761 gcgaactcct cctgtcggcg ccaggtaacg atcacccgcg ccgatgcccg aaggaacgca
     2821 aggctcactg cgcggcccag tccgcccgtg cctccggcga tcaatgcaac tttgcctcga
     2881 aattgcgccg ccatccgact ccttcttttc cgatcattcc gcgctgccga ttttccgcat
     2941 cgcggccaaa aacagattct acgggaccgt cccacccgtc acctgatcct tgacatcgca
     3001 tgcctaaaag ctatatagct gttcacatag cgacgtgata gacggctggc agacgctgcg
     3061 ccagcctccc tggattcccg gaccccgcaa gaccgggttc tgatccctct acttaaatcc
     3121 gacgcacccg catttccgga atctccggga cgtgcggctg atcctttttt caaccggtat
     3181 cccggagagg ggaccggtcg tgttcaaacc ccagcgttct cgctgaaccc tttttggagg
     3241 ttgcacctat ggtggcgcgt tccttttccc gtatcttcgc agtcctgtgt gtaatgggct
     3301 gtctcacctg ttgcagcggt gcgttcgctc aggcaattac cggtacgatc aatggctcgg
     3361 tccacgatgc cagcggcgct gcgattactg gcgctgcggt caaaatacag gacacggtcc
     3421 acaacgttca ggcccgtgct gtgcagacgg acgcgaacgg aaactacacc gctccgcagc
     3481 tccagcccgg catgtacgcc ataacggcca ccgcccaggg cttcaagtct gccagcgtcc
     3541 tcgacgttca cctcaacgtc ggcgacacgc tggccatcaa tctcacgctt ccagtgggaa
     3601 gcagcaccga gacagtgacc gtgtcggcga gcgatgttca ggtcaacacc cagtctgcga
     3661 gcgagagcac cctgatcaac ggagcgcaga tggtgggtct gcccctgaat aatcgcaact
     3721 acgaacagat ggtcggccta cagcccggtg tcgcctacgg cggcggcgat caactctata
     3781 tcggtctgtc gaatccgtcc ggggaaacca acgtcgtcgc gttttccatc aacggtcagc
     3841 gcagcagcgc caatacctgg actgtggatg gagccgacaa cgtcgaccgc ggctccaact
     3901 acacgctgct catctatccc agcatcgacg cgatttccga attcagaacc atccgcaata
     3961 cctacagcgc agagttcgga cgcagcgcca gcggtcagat taacctcatc acgaagagcg
     4021 gcacgaatct gctccacggc gatctgcacg aattcaatcg tggcgattac gacgccgcca
     4081 acacctattc caataaactg agcggcctcg cgcgtccacc gctccactac aacaactacg
     4141 gctacaccat cggcgggccg gtctggctgg gcaagctgta tgacggccgc aacaagacct
     4201 tcttcttctt ctcgcaggaa ttccgccgcg tcatcaccta cagcagcctc accctcgcgg
     4261 gcgatccgac ggccgctatg cgctccggga ccttccctgt cccggtttgc gtggccgtcg
     4321 atgccaacgg aaagtgcacg cagacctcga cgcagatcaa caacatcgat cccaccgctc
     4381 aggcctatat caaggatgtc ttcagcaaga tccctgcagg caacattccc gacagcagca
     4441 ccaacgcaaa catcacgctg aacacaatcg aacgcaatct ctataacgaa acccaggaga
     4501 tcgggcggct tgaccagaac ttcggcaaca agttcagcgt cttcttccgc gtggtggatg
     4561 actccatccc cacggtggag ccgggcggcc tgttccaggg agccggcttc cctggagtag
     4621 gagtgaccag caccaacgcc ccgggcagaa attacctggg acacttcctc tacacgttct
     4681 ccccgaagtt tctggtcgat ggcggatttg cctattccta cggtgcgatc ctttccgatc
     4741 cgatcggcta tctggactcc aaccagtcgc cggacgtgaa gccgaccctg ccttatgtat
     4801 cgactctggg acgcgttccc agcctcacct ttacgggcgg caccgccctg ggcagctatg
     4861 gcccctaccg cgactacaac cgcgaccgca atccctacgc gaacctgacc tacattctcg
     4921 gcaaacattc gctgcgtgcc ggtgcgagct atcactatta ccaaaagtcg gaaaatgccg
     4981 cgggcagcaa cgcaggcagc ttcggcttta ccaacacggg cgctccctcc gggacgggtt
     5041 cctactacca gtcgtgggcg tggttcctgc tcggtcagca ggcttcgttc tcgcaggctt
     5101 cgctggacct cactccggac atccgcatgc gtcaattcga ggcttatctt caggacgact
     5161 ggaaagtcac tcctcgactg accatcaatg ccggcgtgcg ctattcgtac tttcaacagc
     5221 ccacggatgc acacaagatg ctgacgacgt tcgatccact gatctacgat tcgtccaaag
     5281 cgccgacgat cgacagcacc ggacatatct gcaagaccgc gccctgtgcg ggcggtgcgg
     5341 cacccaacgc cggctatgac ccgacaaacg gcattgttgt ggccggaacc tccggagctc
     5401 ccttcggcga tcatcctgcg ccgcagatga ataaagcgtt tgctcctcgc gtcggctttg
     5461 cctatgacct cttcgggaat ggcaagaccg ctatccgcgg cggctatgga atagccttcg
     5521 attcatcgct ggtcggcgac atcgagcaga acatcttcca gaatccgcct tacgtgcaga
     5581 acatttccat caacgccgga agcctttcta acccgctgtc gggaacagtg aatgtgagca
     5641 cggtcccccc ggccctccat gccacgcagt accagtccgc acttccctac gggcagcagt
     5701 tcagcctgga tctccagcac cagtgggctc ccgatctgat ctccgacatc ggctacgtcg
     5761 gctcgctcgg cacgcacctc ctcggcgaag tagacctcaa ccagccgcgt cccggcgcat
     5821 ggcagagcct gggcgttccc agtattaccc gctccaatac cacgctgctg aaccggattc
     5881 gcccatggca gggctacgac gccatcaacg ccatcgagcc gtggttcagc tccaattacc
     5941 actccctgca agccgccgtg cagaagcgat tcaaagacgg ctccctaatt gacgccaact
     6001 acacctggtc gaaggcgctg accaataacc aaaccgatcg cagcacagcc gtgcagaacc
     6061 tgtacaacat cgccggcgaa tacggccccg cgcagtacga catccgccac atgttcaacg
     6121 cggacttcgt ctacgctctg cccttctatc gcacgcagca gggagtgatt ggtcacgtgc
     6181 tcggcggatg gcagctcggt ggcctggtct acatgaacgg cggtgttccc ctcacggtga
     6241 cgaccagcgc agacccggct ggcctcggac tgctggattc tgccagcgcc gcaggtgcgc
     6301 gcccgaatca aaccggaaac ccgaactccg gttccgggat tcacacccgc accaactggt
     6361 tcaacaagag tgtcttcgtc gctgacacca atcccaacct gcccggcaac gaacaccgtg
     6421 gttcggtgcg tgggccagga tggcagcgct gggacctcgc cctctacaag aacatcaaca
     6481 tcggagagcg cgtgaagttc cagttccgcg gcgaaacctt caacaccttc aaccacacca
     6541 actacagcgg cgtgaccgtt tccaacacga gcagtctctt tggatacgtc accagctatc
     6601 gcgatccgcg catcatccag cttggcggga aactgaactt ctaggcccgt cttcgtgatc
     6661 aatcggccgg gattccgaca
//
LOCUS       DASZYN010000132         4400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_134237, whole genome shotgun sequence.
ACCESSION   DASZYN010000132 DASZYN010000000
VERSION     DASZYN010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134237"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VHE33_03480"
     CDS             <1..396
                     /locus_tag="VHE33_03480"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HVT96540.1"
                     /translation="RLEGDIFRAIRLVVDTGVHSQHWTRQQMVDYFHAHSGLDDATIN
                     AECDRYIAWPAQALGYKIGQLKILELRSRAQKALGPKFDIRAFDDQVVDSGALPLDEL
                     DARITAWIGKQSGSQLAPRPASAPSAAAR"
     gene            440..1276
                     /locus_tag="VHE33_03485"
     CDS             440..1276
                     /locus_tag="VHE33_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96541.1"
                     /translation="MKLADRFVDLSTRAVPLRTLVMRRLLQRFPIGSWERRLRSGAVR
                     RPHYAWCLHYAALQAKGLGYRAFTVIELGVAGGNGLLCLCDHAAQIRKELGIEIRIVG
                     FDSGGGLPTTSDVRDLHYLWPAGSFQMDRAALERRLGGRAELVLGNVSETVPGWQPSA
                     QAPLGCVMFDLDLYTSTRDALPLLTKANVLPRIWCYFDDISGYPDMALTDFIGEGAAL
                     HEFNHHPDRAILRDNVSPACVFADQVPEMWHRKMYLYHRLSHPQYDICLSSEKHQLQL
                     TN"
     gene            1342..2166
                     /gene="proC"
                     /locus_tag="VHE33_03490"
     CDS             1342..2166
                     /gene="proC"
                     /locus_tag="VHE33_03490"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HVT96542.1"
                     /translation="MSTDLSTSRVAVLGAGKMGGILLQAFLKQQILSQDRLFATVAHA
                     DRAQVLSAQWGIEVSTDNADIASKADVILIGVKPFQVPALIEEIRPTLTSDKLLISFA
                     ASVRTESIEKTAVEEVPVIRAMPNTPSMLGAGISALCRGRFVQPAHMELAESLFTAVG
                     RTVVVDEKHMDAVTGLSASGPAFIYIILESLAEAGVKVGLPRDIATLLAAQTTFGAAK
                     MVLETGYHPALLKDAVTTPAGCTIDGILELEEGGLRVTLIKAVMRAAQRAKELAAG"
     gene            2846..3079
                     /locus_tag="VHE33_03495"
     CDS             2846..3079
                     /locus_tag="VHE33_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96543.1"
                     /translation="MALNPVGSPASGIGPTPECHAHTPVLATVDGTTYVANVNDSAGM
                     YIAQDPAIFGAEGTGPTAQAAEEAFRNRIDLLA"
     gene            3165..4379
                     /locus_tag="VHE33_03500"
     CDS             3165..4379
                     /locus_tag="VHE33_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HVT96544.1"
                     /translation="MHRLFAPAAALLLALACTPALLAQNSPRWIGTWAASPMGSAVNF
                     GQPSPANTTYRNLVRISAGGTTVRVELTNEFGARPLTIGAAHVALSAGAGAIQPATDH
                     ALTFNGQPSVIIPPGAPMFSDPIPMQVPALASLDVSIYLPDQSIGETTCHQEGMTTTW
                     ITEGDNTAAAAVTNARTISSFCFVKAIDVTAADANSATIVCLGDSITDGAHSTPSTNR
                     RWPDILAARLQADPKTAHLFVLNEGIGGNRLLNDIAGPNALARFNRDVLAQSGVKYVI
                     LLEGINDIGHTTEPRSPADLVTVQQMILAGTQIVTEAHAHGLKIYGATLTPYVGARYA
                     SPKGEEMREAFNQWIRTSGLFDGVIDFDKAVQDPANPTAFAPAYDSGDHLHPNDAGYA
                     KMGDSIDLKLFQ"
BASE COUNT          814 a         1628 c         1100 g          858 t
ORIGIN      
        1 cgcctcgaag gcgacatctt ccgcgccatc cgtctcgtcg tcgataccgg cgttcattcc
       61 caacactgga cccgccagca gatggtcgac tacttccacg cccactccgg cctcgacgac
      121 gccaccatta acgccgagtg cgaccgctat atcgcctggc ccgcgcaggc cctcggctac
      181 aagatcggtc agctgaagat cctcgaactt cgctcccgtg cccaaaaagc cctcggccca
      241 aaattcgaca tccgcgcctt cgatgatcag gtagtcgact caggcgccct gcccctcgat
      301 gaactcgacg ccagaatcac agcgtggatc gggaagcaat ccggttcaca attagcaccg
      361 cggcctgcgt ctgctccttc cgcagctgcc cgctagcctt gcgatactct gcactggaag
      421 aaactctttc ggagaagcta tgaagctggc tgaccgcttc gtcgatcttt ccacgcgcgc
      481 cgttccgctg cggactctcg tcatgcgccg tctcctgcag cgttttccca tcggttcctg
      541 ggagaggcgc cttcgctccg gtgctgtccg ccgtcctcac tacgcatggt gtctccacta
      601 tgcggctctt caggcgaaag gcctcggcta ccgcgccttc accgtcatcg aactcggcgt
      661 cgctggcggg aacggtcttc tttgcctctg tgaccacgcg gcgcagatcc gaaaagaact
      721 cggcatcgaa atccgcatcg tgggattcga ttccggcggc ggcctcccca cgacttccga
      781 tgtccgcgat ctccactatc tctggccggc cggttctttc cagatggatc gcgccgcact
      841 cgaacggcgc ctcggcgggc gcgctgaact ggtcctcggc aacgtctcgg aaaccgtccc
      901 cggttggcag ccctccgccc aagcgcccct cggatgcgtc atgttcgacc tcgatctcta
      961 tacctccacc cgcgatgccc tgcccctgct caccaaagcc aacgtccttc cccgcatctg
     1021 gtgctatttc gatgacatca gtggatatcc tgatatggct ctgaccgact tcatcggaga
     1081 aggagcggcg ctgcacgaat tcaaccatca tcccgatcgg gcaatcctgc gagacaacgt
     1141 ttccccggcg tgcgtatttg ccgatcaggt tccggagatg tggcaccgga agatgtacct
     1201 ctatcaccgt ctctcgcatc ctcaatacga catctgcttg tccagcgaga agcaccagct
     1261 ccagctgacc aattgaaagg acggccgtcg cagtcagaac gggatgtgcg ccctggaccc
     1321 cgcaccctgt accctgattc catgtccacc gatctttcca cctcccgcgt cgccgtcctc
     1381 ggcgcaggca aaatgggcgg cattcttctc caggcgtttc tcaaacagca gatcctgtcg
     1441 caggaccgcc tcttcgccac cgtcgcccac gccgaccgcg ctcaggtcct ctccgcccag
     1501 tggggcatcg aagtctccac cgacaacgct gacatcgcct ctaaagccga tgtcatcctg
     1561 atcggtgtga aacccttcca ggttccagcc ctcatcgaag agatccgccc caccctcacg
     1621 tccgacaaac tgctcatctc ctttgccgca tccgtccgca ccgaatccat cgagaaaacc
     1681 gccgtcgaag aagtccccgt cattcgcgcc atgcccaaca cgccttcgat gctcggcgcc
     1741 gggatctccg ccctctgccg cggccgcttc gtgcagccgg cacacatgga gctcgccgaa
     1801 agcctcttca ccgccgtcgg ccgtaccgtc gtcgtcgacg aaaagcacat ggacgcagtc
     1861 accggactca gcgccagcgg acccgccttc atctacatca ttcttgagtc gctcgccgaa
     1921 gccggcgtca aggtcggcct gccccgcgac atcgccaccc tgctcgctgc gcaaaccacc
     1981 ttcggcgctg ccaaaatggt tctcgaaacc ggctaccacc cggcccttct caaggacgcc
     2041 gttacaaccc ccgccggctg caccatcgac ggcatcctcg aactggaaga aggcggtctc
     2101 cgcgtcaccc tcatcaaggc ggtcatgcga gccgcccagc gcgcgaagga attggccgcg
     2161 ggatagccgt cggagcacga gccactgtaa cccacgctgc cgtatttcta tactagttaa
     2221 tactagtgtt attagcttat ctatgatttc attacacccg cagtaaccca ttactaaacg
     2281 gttagtgcaa gtgggagagt aacggccact ttccggcgat gtcttccccg ccgccttcag
     2341 gctaccctca ggcgttggtt gttgtgctgg ttgaaccttg taccactttt tgccatggaa
     2401 acaacaagca tgtactggaa ggttgctgct gcggtcctga ttgtttttgc cctgcgttgg
     2461 cttgcacgcc gctttcgcga tcccgacacg ttctgggaat gatcgacgcg agagcgtagc
     2521 cacagcctgg taagacccga tttccgccaa aacccggcct ctgcgttccc tggccgcact
     2581 gtcgatgcgc tcgcgccttg cgggcgctca cagctccgca tccagcggcc agcatcatcc
     2641 attcctcctc cacgaatctc ccttccagtg ccctctgcgc ggtctttcaa ccgccgggtt
     2701 cgctaccgat ccatgcgcat ttcccaaagt gggattgcgg cgtgccgatc gttgcaggta
     2761 ccgctttttc cactgaaact ttcccgggaa ctgccgatat atccggctag cgatagcgca
     2821 ttcccaaaac gacgaggatg cttccatggc tttgaaccct gtcggctctc ccgcctccgg
     2881 aatcggacct acaccggagt gccacgcgca cacccccgtc ctcgccaccg tcgatggcac
     2941 aacctatgtg gcgaacgtca acgattcagc cggtatgtac atcgctcagg accccgccat
     3001 ctttggcgcc gagggcaccg gcccgactgc ccaggccgct gaggaagcgt tccgcaatcg
     3061 catcgatctt ctcgcctgag tccgccgccg caaggggttc ctcgccctcc gtgcgcgtga
     3121 acgtcacttc gtgtaaaagg gtccaagtcc attctggagc cctcatgcac cgtctcttcg
     3181 ctcccgctgc tgctctgctg cttgctctgg cctgcacccc cgcactcctc gcgcagaact
     3241 ccccccgctg gatcggcacc tgggctgcct ctcccatggg ctcagccgtg aattttgggc
     3301 agccatcccc ggccaacacc acctatcgca atctggttcg catcagcgcc ggcggcacta
     3361 ccgtccgtgt cgaactgacc aacgagttcg gcgcccgccc cctcaccatc ggcgccgccc
     3421 atgtcgccct tagcgctgga gccggcgcca tccagcccgc caccgatcat gccctcacct
     3481 tcaatggaca accctccgtc atcatcccgc ccggcgcccc catgttcagt gatccgatcc
     3541 cgatgcaggt cccggccctt gccagtctcg acgtcagtat ttacctgccc gatcagtcca
     3601 tcggtgaaac cacctgccac caggaaggca tgaccaccac ctggatcacc gagggcgaca
     3661 acacggcggc cgcagccgtc acgaatgctc gcaccatctc gtccttttgc ttcgtcaagg
     3721 ccatcgacgt cacagccgct gatgcaaact ccgccaccat cgtctgcctc ggcgactcca
     3781 tcaccgacgg cgcgcattcc acacccagca ccaatcgtcg ctggcccgac atcctcgccg
     3841 ctcgcctcca ggccgatcca aaaacggccc acctcttcgt cctcaacgag ggcatcgggg
     3901 gcaaccgcct gctcaatgac atcgccgggc ccaacgcgct ggcgcgcttc aaccgcgacg
     3961 tgctcgcgca aagcggtgtg aagtacgtca tcctgctcga aggcatcaac gacatcggac
     4021 ataccaccga gccccgcagc ccggccgacc ttgtcaccgt ccagcagatg atcctggccg
     4081 gcacccaaat cgttaccgaa gctcatgcgc acggcctgaa gatctacggc gccacactga
     4141 ccccgtacgt cggagcccgt tatgcctcgc ccaaaggcga ggagatgcgc gaagccttca
     4201 atcagtggat tcgtaccagc ggcctcttcg acggcgtcat cgacttcgac aaggcggtcc
     4261 aggacccggc aaatcccacc gccttcgccc ccgcctatga ttcaggcgac cacctgcacc
     4321 ccaacgacgc gggttacgca aagatgggcg actccatcga cctcaagctc tttcaatagc
     4381 cggcaatcgg ccgggaaccg
//
LOCUS       DASZYN010000133         8497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_646134, whole genome shotgun sequence.
ACCESSION   DASZYN010000133 DASZYN010000000
VERSION     DASZYN010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8497
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646134"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="VHE33_03505"
     CDS             complement(<1..1169)
                     /locus_tag="VHE33_03505"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVT96545.1"
                     /translation="MMLKRFRPTKLLRPWGGGGALALRTAVTVAVMLFAVSAWAAEYH
                     GRVFYAGVPIPGATVTLSQGSQQVATVTDEQGIYEFPDVADGAWKIEIRMRGFQTFTS
                     QVNIAPNQPQGAWELQLLDLQQMLAQSKEVKPVAEPQLAQRGEEQPKPKAQNGTDANA
                     AMPPAGDEAEKAEDGLLINGTSNNANTSRFSVSPSFGNHRPGTRGLYTGGFGAIVSNS
                     LFDARPYSLTGLQLPKASYSRVTGLATLGGPLNVPHLFYHGPDFFLAYQWTRNSNAVT
                     DPALVPTAAERAGDLSGLVNAQGQPATVYDPSTGLPFTGPIPVSPQAAALLQYYPLPN
                     ISGNARYNYEAQVLNHTHSDALESRLQKTLGRRDQLYGGIGFESSRGDTANVFNFR"
     gene            complement(1368..2588)
                     /locus_tag="VHE33_03510"
     CDS             complement(1368..2588)
                     /locus_tag="VHE33_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HVT96546.1"
                     /translation="MAGIPKWSIRRRKASGLLALAVIATLFAADPIRGQAPAQSDPAA
                     QQTAAPTATNSIAGTWQGTLHAGRDLRIVVKITNEGGGVKATMHSIDQGGQGIPSSSA
                     SFAGGTLKFAIQFLDASYEGKLSGDGRSIAGTWTQADHPLPLVLERATPETEWTIPEP
                     PPRVPPMAADAKPGIEVATIKPSQPGEQRRFITFRGREIVIVGFTLNDVIKFAYDVQE
                     KQILNGPAWLGSDRFDINAQPDQPGTPSPDQFRSILQKLLTDRFALKFHKEKKEMSAY
                     VLTVGKDGPKMTKATDPSGLPGLFFGPLGTLHVVNATMGDFCGLMQSAVLDRPVVDRT
                     GLDGKWNFILKWTPDESQFTGLGVKVPPPTDAADAAPPLFTAIQEQLDMKLDAQKTAV
                     DVMVLDHVDHPSPN"
     gene            2785..5334
                     /locus_tag="VHE33_03515"
     CDS             2785..5334
                     /locus_tag="VHE33_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HVT96547.1"
                     /translation="MRRPIFSSLCVLLLVSAFVSRLVFAQPNPAQPVVLNRLTGSSAL
                     PNGIDLRSGEAREQITALRDDVIRIRVSRSDELPEDASWAVLESARHSTASVTPIATD
                     SIIGFNTKALRIEVDRRTLLLTIRDAKGNILQQDAQPVAWRGATFRIFKKMPLDEHYF
                     GLGDKTGPLDRRNEAFTLWNTDAYRFQESTDPLYKSIPYVLTYRAGVACGFLLDNTWR
                     TNFDFGKLSRDTYSYGAENGPLDEYFFYGPSPKQVVETYAWLTGTAPLPPLWTLGYQQ
                     SRYSYYPQSRVLEVAARLRADHIPADSIYLDIDYQQDYRPFTVNKTYFPDFPGMIAKL
                     NAENFHTVAITDLHIANYPGHGYAPYDTGIAGDEFVKNPDGSVFTGRVWPGPSVFPDF
                     TQARTRVWWGNLYRDFYSMGIAGFWNDMNEPSVFDSPTQTMPVDVVHRIDEPGFRKRT
                     ATHAEIHDVYGMENSRATFEGLLTLKPDQRPFVLTRASYAGGQRYAATWTGDNSSSWN
                     HLRMTTPMLENLGLSGFAFAGADVGGYAGTPSVDLLTKWLEVAAFQPIDRDHTEKGSG
                     DQEPWVGGPEQEAIRRHFIETRYRLMPYLYTVAEEASRTGLPMVRPIFLEFPDAAADR
                     HPLDVELTASAEFLLGSDLLIAPAPWPDQKEDYPVELPSRDWYDFWTGAKIPQPPPPP
                     PASEHDPVNPAAPPLTTTAHAELATLPVFVRGGTILPMQPLVQSTNETPQGPLELHVY
                     PGDAGEGRACGGSFYLDDGNTMAYRRGDFLRMRFTCSDSAGKLQIHIGAHEGSYAAWW
                     KQLRIEVYGSSAEAATVLTGGVRRSAAVARKGQAVVFDVDDNGKGFDVELQ"
     gene            complement(5505..6551)
                     /locus_tag="VHE33_03520"
     CDS             complement(5505..6551)
                     /locus_tag="VHE33_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HVT96548.1"
                     /translation="MDIHAVARQAHVSIATVSRTINHVPSVNPKLAKRVWEAIRELNY
                     FPNTQARALVSGRSRLLGLIVSEITNPFFPELIQGFEEVAVENGYEILIGSTNYEPDR
                     MQRCIRRMVERNAEGVAVMTFGIEQPLLDQLTDRNVPLVFVDVAPNRPGMSVLKVDYH
                     HGIRQGVQHLAALGHRRIAFVSGPLRLHSAQSRLGAFKKALGECGIPVDETLMVEADH
                     TMEGGMAAAANLMAHKDPPTAIMCSNDMLAIGALHECSRSGLRVPDDVSIIGFDDIHM
                     AAMIIPPLTSIQMSRADLASAAVSALRGHLEGAAKREYQIDTRLVVRESTGFPRGTMA
                     HLRGNKEKAVAASG"
     gene            6742..8328
                     /locus_tag="VHE33_03525"
     CDS             6742..8328
                     /locus_tag="VHE33_03525"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HVT96549.1"
                     /translation="MTIVAGVDFGTLSVRVSLLSSDRGRLGTAVAEYPLHRKREDPDF
                     ATQSHADHMRALAEATRAAVKSAGIQGDQIAAIALDTTGSSVVPVDERMQPLSEYYLW
                     CDHRAKSEAQEITALAHKEKLEAIEWCGGVYSHEWGFAKLLHWLRHNPEKRGQFASAF
                     EHCDMVAATLCGITDPRQAKRSVCAMGHKWMWNPKWDGLPPQSFLSKVDPLLDGIRDK
                     LAGDYQTSDHLAGHLTPEWAEKLGLRAGIPIPVGAFDAHWDALGAGCREGDVVNVVGT
                     STCIVAIAKKTQLIPGVCGVVPGSVHPQYTGIEAGLSATGDIFEGIARRAGVRVAELS
                     RGLEKYRAGQTGLLRLSWDNGDRTVLVNSELGGVTLGWNLVHTAQDELFAAIEGTAFH
                     TRIILERMAEHGTRVDRVINGGGIPQNNAVLNQVYADVLGKPVLVPSEIPTSLGSGIF
                     AMLAAGAFPSIDAAQEKLCPKFTEFRPQARASSICERLYPLYRKVYFALGTRSAEPAA
                     LGDVLPEVRKIAAEVNSLQG"
BASE COUNT         1605 a         2746 c         2456 g         1690 t
ORIGIN      
        1 cgaaaattga agacgttcgc cgtatcgccg cgtgagcttt cgaagcctat gccgccgtag
       61 agttggtccc gccgccccag cgtcttttgc agacgcgact cgagcgcgtc gctgtgcgta
      121 tggttcagca cctgcgcctc atagttgtag cgcgcgtttc ccgaaatatt cggcaacgga
      181 tagtactgca gcagagcagc cgcttgcggg ctgacgggaa tcggtccggt gaatggcagg
      241 cccgtcgatg gatcgtacac ggtcgccggc tggccttgcg cattcaccag gccggaaaga
      301 tcgcccgcgc gctcggcggc cgtcggcacc agcgccggat ccgtcaccgc attgctgttg
      361 cgcgtccact ggtacgccag gaaaaagtcc ggcccgtggt agaagaggtg cggaacattc
      421 aacggccccc ccagcgtggc aagaccggtc acgcggctgt acgaagcttt gggcagttgg
      481 agtccggtca gtgaataagg ccgggcgtcg aaaagcgagt tgctgacgat ggccccgaac
      541 ccgcccgtgt agagtccacg cgtccctgga cgatgattgc cgaaggatgg cgacacggaa
      601 aatctcgacg tgtttgcgtt gttcgaggtc ccattgatca gcagaccgtc ttcagccttc
      661 tccgcttcat cgccagcggg aggcatcgcc gcattcgcat cggttccgtt ttgcgctttg
      721 ggttttggct gctcctcccc acgctgtgcg agctgcggtt cggcgaccgg tttcacttcc
      781 ttgctctgag ccagcatttg ctgcagatcg agcagctgta gctcccatgc gccctgcggc
      841 tggttgggcg caatgttcac ctggctcgtg aaggtctgaa aaccacgcat gcggatttca
      901 atcttccatg caccgtcagc cacgtccgga aattcgtaga ttccctgctc atcggtgacc
      961 gtcgcaactt gctgtgagcc ctgcgacagg gtgacggtcg cgccaggaat cggaacgccc
     1021 gcgtaaaaga cccggccgtg atactccgcc gcccacgccg acacagcaaa gagcatcacg
     1081 gccacggtga ccgccgtgcg cagagccagg gcgccgccac cgccccatgg gcgcaggagt
     1141 ttcgttggcc gaaagcgttt cagcatcatt cgtctacaga aagagcaacc aggcttccgg
     1201 ggaatcggtc atccaacatc cacctcagcc accatcgttg agcgtttgcg ctgtccaaca
     1261 gacgcatgtt ccggtccatc ggtcaacgac tcactgccct tcgacgtcgg attcagaagg
     1321 cctgtccgtt gccggacaat gaatcgctcc aggaccgtgc gatccattta gttcggcgaa
     1381 ggatggtcca cgtgatcgag aaccatcacg tccacggccg ttttctgcgc atccagcttc
     1441 atgtccagtt gctcctggat tgcggtgaac agcggcggcg cagcatcggc cgcatcggtc
     1501 ggcggcggaa ccttgacgcc gagtcccgtg aactgcgact cgtccggcgt ccacttgaga
     1561 atgaagttcc atttgccgtc aaggcccgtg cgatccacga ccggccggtc cagcaccgcc
     1621 gactgcataa gtccgcagaa gtcgcccatc gtggcattga ccacatgcag agtgcccagc
     1681 ggtccaaaga ataatccggg caggccgctc ggatcggtgg ccttcgtcat cttcggcccg
     1741 tctttgccca cggtcagaac gtaggccgac atttctttct tttccttgtg gaacttgagc
     1801 gcgaagcgat ccgtcagcag tttctgcagg atcgacctga actggtcggg gctcggcgta
     1861 cctggctgat cgggctgcgc attgatgtcg aaacgatccg accccagcca cgccggccca
     1921 ttcaggatct gcttctcctg cacgtcgtaa gcgaacttga ttacgtcgtt gagcgtgaac
     1981 ccgacgatca cgatttcgcg cccgcggaag gtgatgaacc gtcgctgttc gccgggctgg
     2041 ctgggtttga tggtcgcaac ctcgatgccc ggcttcgcat ccgctgccat cggcggaacg
     2101 cgcggcggag gctcaggaat cgtccattcc gtctccggcg tcgcgcgctc cagcaccaga
     2161 ggcagcggat gatcggcctg cgtccaggtg cctgcgatcg acctgccgtc gcccgacagc
     2221 ttgccttcat agcttgcatc aaggaactgg atggcgaact tcaatgtgcc gccagcaaag
     2281 ctggccgacg aagatggaat cccctggccg ccctggtcga tgctgtgcat cgtggccttc
     2341 acgccgccgc cctcgttcgt aattttgacc acaatgcgca ggtcgcgacc ggcgtgcagc
     2401 gttccctgcc aggtgccggc gatgctgttc gtcgcagttg gtgcggcggt ctgctgcgct
     2461 gccgggtccg attgcgccgg ggcttggccg cgaatcggat cggccgcgaa cagagtcgcg
     2521 atcacggcga gcgcgagcag accagacgcc tttcgcctcc ggatcgacca ctttggaata
     2581 ccggccattg ccaacagccc ctttcctctc gccgaatcgg ctttcggcgc acgcacccaa
     2641 atggaagtag ttgcccgcca gaatagcact ctcaccgaac gagttacctc ccgaagttat
     2701 gcccgttcgt cactcatttg caacgctgcc ttgacgcatt cgtctggcga gacgctcgcc
     2761 ctggcagtaa gattcaggat tgttatgcgt cgccccatct tctcgtcgct ttgcgtcctt
     2821 ctcctcgtct ccgcctttgt gtcgcgtctg gtcttcgcgc agccgaatcc tgcgcaaccg
     2881 gtagtcctta accgcctgac tggatcctca gccctcccca acggcattga tctgcgttcc
     2941 ggagaagcgc gcgagcaaat caccgctctc cgcgacgatg tgattcggat ccgcgtgagc
     3001 cgtagtgacg aacttcccga agacgcatcc tgggcggtcc ttgaaagcgc gcgccacagc
     3061 accgcatccg tgacgccgat cgcgaccgat tccatcatcg gcttcaacac gaaggctctc
     3121 cgcattgaag tcgatcggcg caccctgctg ttgaccattc gcgatgccaa aggaaacatc
     3181 ctgcagcagg atgcccagcc tgtcgcctgg cgcggcgcaa cgttccgcat ctttaaaaag
     3241 atgccgctcg acgaacacta cttcggactg ggagacaaga ccggcccgct cgaccggcgc
     3301 aacgaggcct tcacgctctg gaataccgat gcttaccgtt ttcaggaatc cacagatcct
     3361 ctttataagt cgattcccta tgtgctgact tatcgcgccg gcgttgcctg cggatttttg
     3421 ctggataata cctggcgcac caacttcgat ttcggtaagt tatctcgcga cacttactcc
     3481 tacggcgccg aaaatggtcc tctggacgag tatttcttct atgggccgtc gccgaaacaa
     3541 gtagtcgaga cctacgcctg gctcaccgga accgcgcccc tgcctcctct ctggacgctc
     3601 ggttaccaac aatcccgcta cagctactat ccccagtccc gcgtgctgga agtggctgcg
     3661 cgcctgcgtg ccgaccatat tccggccgat tccatctatc tcgacatcga ttatcagcag
     3721 gattatcgcc ccttcaccgt caataagact tactttcccg atttccccgg catgatcgca
     3781 aagctcaacg ccgagaattt ccataccgta gcgatcaccg acctgcatat tgcgaactat
     3841 cctggccacg gctatgcgcc ctacgacacc ggaatcgccg gcgatgaatt tgtgaagaat
     3901 cccgatggat ccgtcttcac cggacgcgtt tggccaggcc cctccgtctt tccggatttc
     3961 acccaggccc gcacccgcgt gtggtggggc aatctgtacc gcgatttcta ctcgatgggc
     4021 atcgctggtt tctggaatga catgaacgag ccgtcagtct tcgacagtcc aacgcaaacc
     4081 atgccggtcg atgtagtgca tcgcattgat gaaccgggct tccgcaagcg caccgcgacg
     4141 cacgccgaga ttcacgacgt ctacggaatg gaaaattcac gcgctacatt cgaaggcctg
     4201 ctcactctca agcccgatca gcggcccttt gtgctgacgc gcgcctccta cgccggcggc
     4261 cagcgctacg ccgccacatg gacgggcgac aacagcagca gttggaatca tctccgcatg
     4321 actacgccca tgctcgagaa tctcggcctg agcggattcg ccttcgcggg cgccgacgta
     4381 ggcggatacg ccggaacgcc ctccgtcgat ctgctgacga aatggctcga ggtcgctgcg
     4441 ttccagccca tcgatcgcga ccacaccgag aagggcagcg gcgatcagga gccctgggtc
     4501 ggcggtcccg agcaggaggc gatccgccgc cacttcatcg agacgcgcta tcgtctgatg
     4561 ccgtatctct atacggtggc tgaagaggct tcacgcaccg gactgccgat ggtgcgcccg
     4621 atctttctgg aattccccga cgctgcggcg gatcggcatc cgctggacgt cgaactgacc
     4681 gcttctgcag aattcctgct cggctccgat ctgctcatcg cgccggcgcc ctggccggat
     4741 cagaaggagg actatccggt cgagctgccg tcccgcgact ggtacgactt ctggaccggc
     4801 gcaaagattc cgcaaccgcc tccaccgccg cctgcatcgg agcacgaccc tgtcaatcct
     4861 gccgctccgc cgctgaccac tacggcccat gcggaactgg ccactttgcc ggtatttgtt
     4921 cggggtggca ccatattgcc gatgcagccg ctggtgcaga gcacgaatga gacgccgcag
     4981 ggtccgctgg aactgcacgt ctatccgggt gatgccggcg agggacgcgc ctgcggcgga
     5041 agtttctatc tcgacgatgg caacacgatg gcctatcgcc gcggcgattt tctcaggatg
     5101 cgtttcacct gctcggactc agcgggcaag ctccagatcc atatcggggc acatgaaggc
     5161 tcgtatgccg cgtggtggaa acagctgcgg atcgaagtct acggctcaag tgcagaagcg
     5221 gcgaccgttc tcacgggtgg agtccgccga tccgcagcgg ttgcacgcaa aggacaggcc
     5281 gtggtcttcg atgtggacga caatggaaaa ggattcgatg tagagctgca gtaatgcgca
     5341 cgcgtgggtc gttttcgaat tctctttact ctgtcatccc aaccgggccc tgagcatagc
     5401 gaaggggaag cggagggacc tgcttctcct tgtcatcccg accgaagcgg agggacctgc
     5461 ttctccccga acgcgcacaa caaagaacct tcggcaacac cgcgctagcc cgaggccgcg
     5521 accgccttct ctttgttccc gcgcaggtgc gccatggtgc cgcgcggaaa tcccgtcgac
     5581 tcccgcacca ccagccgcgt gtcgatttgg tattcccgtt tggccgcgcc ctccagatga
     5641 ccgcgcagcg ccgagaccgc ggccgacgcg agatcggccc gcgacatctg gattgaggtg
     5701 agcggcggaa tgatcatcgc ggccatgtga atatcgtcga acccaatgat ggaaacatcg
     5761 tcgggcaccc gcagccccga acgcgaacac tcgtgcagcg cgccgatggc cagcatgtcg
     5821 ttggaacaca tgatggcggt gggcggatcc ttgtgcgcca tcagattcgc ggccgctgcc
     5881 atgcctccct ccatcgtgtg gtcggcttcc accatcagcg tttcgtcgac gggaataccg
     5941 cattccccca gcgctttctt aaaagccccc aggcgagatt gcgccgagtg cagccgcagc
     6001 gggccgctca caaaggcgat gcgccggtgt ccgagcgccg cgagatgctg cacgccctga
     6061 cgaatgccgt ggtggtagtc caccttcaga acgctcatcc ccggacggtt cggagcaaca
     6121 tccacaaaaa caagcggcac attgcggtcc gtcagctgat cgagcagcgg ttgctcgatg
     6181 ccgaaggtca tcaccgcaac gccctcggcg ttgcgctcca ccatgcgccg gatgcagcgc
     6241 tgcatgcggt ccggctcata gttcgtggac ccgataagaa tctcgtagcc gttctccacc
     6301 gcgacttcct cgaagccctg aatcagttcg gggaaaaatg gattcgtgat ttctgaaacg
     6361 atgagcccca gcaggcggct gcgtccggac accagcgcgc gcgcctgggt attaggaaag
     6421 taattcagtt cccggatggc ctcccacacg cgcttggcca gcttcggatt gacgctgggg
     6481 acgtggttga tggttcgtga tacggttgca atcgacacat gcgcctgtcg agcgactgcg
     6541 tggatatcca tgcgatcggg tctcgcagcc gagcgccgtc tggttcttct cgttgggcca
     6601 gccataagtg cgcagaatgc gtttacagca tactccatcg ctaactcatg ccatacaata
     6661 gttgcgcgct tttagtaccc ggaaaatgtt tactccggga gcctaactaa ttgaattgct
     6721 atctttcgat tggatattcc catgactatc gtcgcaggcg tggacttcgg cactctctcg
     6781 gtccgcgttt ctctactttc cagcgatcgc ggccgcctgg gcacggcggt cgccgagtac
     6841 ccactccatc gcaagcgcga agatcccgat ttcgccactc agtcgcacgc cgatcacatg
     6901 cgcgccctcg ctgaagccac tcgcgccgcg gtgaagtcgg ctggaatcca gggcgaccag
     6961 atcgccgcca tcgcgctcga caccaccggc tccagtgtcg tccccgtgga cgagcgcatg
     7021 cagccgctca gcgagtacta cctgtggtgc gatcatcgcg ccaaatccga agcacaggaa
     7081 atcacagcgc tcgcccacaa ggagaaactc gaagcgattg aatggtgcgg cggtgtctac
     7141 tcgcacgagt ggggattcgc caagctcctg cactggctcc gccacaaccc ggaaaagcgc
     7201 ggccagtttg cctcggcttt cgaacactgc gacatggtgg cggccactct gtgcggaatc
     7261 accgatccgc gccaggcaaa gcgcagcgtc tgcgcgatgg gccacaagtg gatgtggaat
     7321 ccaaaatggg atggcctgcc gccgcagtct ttcctttcga aggtcgatcc gctgctcgac
     7381 ggcattcgtg acaagctcgc cggcgactat cagacatcgg atcatctcgc cggccatctg
     7441 actcccgagt gggcggagaa gctcggcctc cgtgccggta ttcccattcc cgtcggcgcc
     7501 ttcgatgccc actgggatgc gctgggcgcg ggttgccgtg aaggcgacgt ggtcaacgtc
     7561 gtcggcactt ccacctgcat cgtcgccatc gcgaaaaaga cgcagctgat tcccggcgtg
     7621 tgcggcgttg tgcccggaag tgtgcatccg cagtacaccg gcattgaggc cgggctctcc
     7681 gccaccggcg atattttcga agggatcgcg cggcgtgcgg gcgtcagggt cgccgagctt
     7741 tcgcggggtt tggagaagta ccgcgccggc caaaccggcc tgctgcggct ctcgtgggac
     7801 aacggtgatc gcaccgtgct cgtcaactcc gaactgggcg gcgtgactct cggctggaac
     7861 ctggttcaca ccgcacagga tgaactgttc gcggcgattg aaggcaccgc cttccacact
     7921 cgcatcattc tggagcgcat ggccgagcat ggcacgcgcg tcgaccgcgt cattaacggc
     7981 ggcggcattc cgcagaacaa tgcggtactg aatcaggtct acgcagacgt tctgggcaaa
     8041 ccggtgctgg tgccttcgga aattcccacc agcctcggct ccggcatctt tgccatgctg
     8101 gcggcgggag cattccccag catcgacgca gcccaggaaa agctttgccc gaaattcaca
     8161 gagttccggc cgcaggcccg ggcttcatcc atttgcgaac ggctttatcc gctctatcgc
     8221 aaggtctatt ttgcgctcgg gactcgctcg gcggaaccgg cagccctggg tgacgttttg
     8281 ccggaggtaa gaaaaatcgc tgctgaagtc aacagcttgc agggctaact tcgaagacgg
     8341 cgccgaagat cgactgaagt cccgaacgtg agcagacaac gcctcaaacc ttcgccgcgt
     8401 ccatggaggc gccaggttca ccccacagca agcagaagac tctttcgtct atgactagat
     8461 caattgattt cagcccccga ccagcgccga agtcaca
//
LOCUS       DASZYN010000134         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_6156, whole genome shotgun sequence.
ACCESSION   DASZYN010000134 DASZYN010000000
VERSION     DASZYN010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6156"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VHE33_03530"
     CDS             <1..810
                     /locus_tag="VHE33_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96550.1"
                     /translation="QQIVSSGLAAPGDWEAILAILIASGQLSNSVLTQPFGVFGGGLT
                     MTGVAYQGGSLNMKLNSSDVRSLDQLQLRVLDREEATIKAGERYPIETSNYSSLGSTQ
                     NLNIPGLSNAGLSSTLQNLGITPQELQAQASMAIPQVEYQDIGLSLIVTPQLEGTDRV
                     SMKFHLTLSALAGSSINSLPVLTNREYNAITSMKVGETALLVSSITRQESNAITGIPG
                     LSELPGFQSTTNRNSNIDVGELAIVITPHIVRSVNRPASEKMILLPVGRPQ"
     gene            855..1223
                     /locus_tag="VHE33_03535"
     CDS             855..1223
                     /locus_tag="VHE33_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96551.1"
                     /translation="MIDHLHRRTLRALALALTAVWVVTLAFADTAVTQAMHCQRGHMP
                     CCPQSSSSESCSTARCAEQIPEKAEAQLTQDQEDEIQAVVPPVPPAIAPRLTPEFVRE
                     LTAGLRYQPAVFRLKDDLRI"
BASE COUNT          307 a          505 c          439 g          316 t
ORIGIN      
        1 cagcagatcg tctccagcgg cctggcagca cccggcgact gggaagcgat tctggccatc
       61 ctcattgctt ccggccaact cagcaactcg gtgctcaccc agcccttcgg cgtctttggc
      121 ggcggactca ccatgactgg cgtcgcctac cagggcggat cgctgaacat gaagctgaat
      181 tcctccgacg tgcgctccct cgatcagctg cagctgcgcg ttctggatcg agaagaggcc
      241 accatcaagg ctggcgaacg gtaccccatt gagacgtcaa actactccag cctgggatca
      301 acccagaacc tgaatattcc agggttgtcg aatgcgggac tctccagtac gctgcagaac
      361 ctgggcatca ctcctcagga actgcaggcc caagcctcga tggccattcc tcaggttgaa
      421 taccaggata tcggcctgag cctaatcgta acgccacagc ttgagggcac ggaccgcgtg
      481 tctatgaaat tccaccttac cttatcggcg ttggccggtt cctcgatcaa cagcctgccc
      541 gtcctcacca atcgcgagta caacgcgatc acctcgatga aggtcggcga gaccgcgctg
      601 ctcgtcagtt ccatcacccg ccaggaatcc aacgctatta ccggaattcc aggactgagc
      661 gagctccccg gctttcagag caccacgaac aggaacagca acatcgatgt tggcgagctg
      721 gcgattgtga ttacgcccca cattgtccgg tcggtgaatc gccccgcttc ggagaagatg
      781 attttgttac cagtagggcg gccccagtga cggagatcac tttgccggct ttccctcagc
      841 tctagactga ggctgtgatc gaccacctgc atcggcgcac cttgagagcc ctggcactgg
      901 cgctcacagc cgtgtgggtg gtcaccctgg cctttgccga taccgccgtg actcaggcga
      961 tgcattgcca gcgcgggcac atgccctgct gcccgcaatc gagcagcagt gagagctgtt
     1021 cgaccgcacg ctgcgcagag cagatacccg aaaaggccga ggcacaactc actcaggatc
     1081 aggaagacga gatccaggcc gttgttcctc ccgttccgcc cgccatcgcg ccgcggttaa
     1141 caccggaatt cgtgcgggag ctgacggcag ggcttcgcta tcagcctgcg gtctttcgtc
     1201 tcaaagacga ccttcgcatt taaacctcgc agtgtgtcgt gctgtttttt gcgcgcgctg
     1261 atgcgtgccc ggcatttccc ttcacattga gaggagtttt cccatgaaaa cgaaagtctt
     1321 tgctatgttc gcaacgtctc tgtttgctgt ttctgcactg gctgctgtag gcgccgcatg
     1381 ttgcaagcca ggttcggcat gctgcaagcc gggcgccagc tgctgccagg gcagccagaa
     1441 agccgcgtcg cactgcccgg gtgactgctg cggcggaagc tgccccggcg gctgctgcgg
     1501 cggcaattaa ccatcgttcc aacaaacagc gccccgcgtt ccggctcacc ggaacgcggg
     1561 ggtcctt
//
LOCUS       DASZYN010000135         1637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_70135, whole genome shotgun sequence.
ACCESSION   DASZYN010000135 DASZYN010000000
VERSION     DASZYN010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70135"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VHE33_03540"
     CDS             <1..682
                     /locus_tag="VHE33_03540"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HVT96552.1"
                     /translation="RRLRYTRSMSSEQGGLFGGVNPQRGGAEWVTAYCDGGSRGNPGP
                     AGYGVFVEGPGGEKLAELSEYLGKKTNNVAEYSGLLAALEWALQSGRRRLRVVADSEL
                     MVKQMQGRYKVASPDLRPLYEEAKRRVAQLEGFRIEHVLRGKNKNADRLANLAMDKGM
                     GRSGTSLKQEAGSRKPEGAPRPGGGVAEGKFEPSRSAKGYVKGGVVHLIEGELPEGAF
                     VRVVVDRQ"
     gene            complement(693..1412)
                     /locus_tag="VHE33_03545"
     CDS             complement(693..1412)
                     /locus_tag="VHE33_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96553.1"
                     /translation="MNAPRSDCKFPSVTPNPQSARGANQKKLHPGRTSEYKHRVSQEG
                     KFWHWFLKHEQELFNLDSNPEPLFTQLISALSRVDRDLTFEIGPGADKRELVISAGGI
                     KRAFPAVSRLVQAAPPQLARWTITAFRPRTPLPITLEIEGKRVSSEDVQFSLLENGSV
                     AGIRLFIHGYNEQSTLFKQFGYLLLDAALGEYDVETGLGFIQMLPPEESTSEHRYPFA
                     DLPELFDQLIARLGKRSGIPC"
     gene            complement(1422..>1637)
                     /locus_tag="VHE33_03550"
     CDS             complement(1422..>1637)
                     /locus_tag="VHE33_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96554.1"
                     /translation="LYRVRATAVIAMLDPRYLDNPSASEARIAAVLRPARHRRHGRAL
                     LRTALYHRRSNRALAKKNSINRSKDKA"
BASE COUNT          332 a          372 c          598 g          335 t
ORIGIN      
        1 ccgccggctt cgttacactc gctctatgtc ctcagagcag ggtgggttgt ttggtggagt
       61 gaatccgcag cgcggtggcg cggagtgggt gacggcgtac tgcgacgggg gatcccgggg
      121 gaatccgggg ccggcgggat atggggtctt cgttgaaggc ccgggcgggg agaagctggc
      181 cgagttgagc gagtacctgg gcaagaagac gaacaatgtc gcggagtact cgggtttgct
      241 ggcggcgctg gagtgggcgc tgcagtcagg acgcaggcgg ctgcgggtgg tggcggattc
      301 ggagctgatg gtgaagcaga tgcagggccg gtacaaggtg gcgagcccgg atttgcggcc
      361 gctgtatgaa gaggcaaagc ggcgggtggc gcagttggag gggttccgga tcgagcatgt
      421 gctgcggggg aagaacaaga atgcggatcg gctggcgaat ctggcgatgg acaaaggtat
      481 gggtcggtca gggacgagcc tgaagcagga agcgggaagc aggaagcctg aaggggctcc
      541 ccgtcccggt ggaggtgtcg cggaggggaa gttcgagccg tcgcggtcgg ccaagggtta
      601 tgtgaaaggt ggcgtggtgc acctgattga gggggaattg ccggaggggg cgtttgtgag
      661 ggtggtggtg gatcggcagt aggggagccg cgtcagcatg gaatccccga tcgctttcca
      721 aggcgcgcaa tgagttgatc aaagagttct ggcaggtccg cgaacggata gcgatgttcg
      781 ctggtcgatt cttccggcgg aagcatttgg atgaaaccaa gcccggtttc cacgtcgtat
      841 tcccccaatg cagcatccag aagaaggtag ccaaattgct tgaatagcgt tgactgctcg
      901 ttatatccgt ggatgaagag gcgaatacca gcgaccgagc cgttttcgag cagagaaaac
      961 tgaacgtctt ctgaacttac tctcttccct tcgatctcga gcgtgatggg aagaggagtt
     1021 cggggacgaa aggcagttat agtccatcgc gcaagttgag gcggtgctgc ctgaacaagt
     1081 ctggacacgg cgggaaaggc tcgcttgatt ccgccggcgc tgataacgag ttcccgtttg
     1141 tccgctcctg gtccaatctc gaaggtcaaa tcacgatcga ctcgactcag agcagagatg
     1201 agctgagtaa acagcggttc tggatttgaa tccaggttaa acagctcttg ttcatgtttg
     1261 aggaaccagt gccagaattt tccctcttgg ctcactcggt gtttatattc cgaggtacga
     1321 ccgggatgca atttcttctg atttgcgccg cgcgcggatt gaggatttgg cgtgactgaa
     1381 ggaaatttac aatcactacg cggtgcattc accggcgacg tctacgcttt gtctttactg
     1441 cgattgatgg agttcttttt tgccagagca cggttagagc ggcggtgata caacgcagtg
     1501 cgcagcagag cccggccgtg gcgacggtgg cgagcggggc ggagcacggc ggcgatgcgg
     1561 gcttccgagg cagaaggatt gtcgaggtag cgcgggtcga gcatggcgat gacggccgtg
     1621 gcgcgcacgc gatagag
//
LOCUS       DASZYN010000136         7683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1160222, whole genome shotgun sequence.
ACCESSION   DASZYN010000136 DASZYN010000000
VERSION     DASZYN010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160222"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..2851)
                     /locus_tag="VHE33_03555"
     CDS             complement(179..2851)
                     /locus_tag="VHE33_03555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT96555.1"
                     /translation="MSLRRYWHRQKWDEERALELEAHLEQETSDNLARGMSQQEARRR
                     ACMKLGNPTTIREEIWKMNSFVSIEDLGRDLRYALRQLWKSPGFAAIAIVTLALGIGV
                     NTAVFSMVNGLLFSSLHIRNERSVVEVGLRQNGESWEPAVSVPEYQTIAAQTRGLFSE
                     VFADQYGLDGIATAGNPPDRLFTDYVTGNYFDALGVKPALGRFFKPTEGATPGADPYV
                     VLSYAYWKDHFGGDAGVVGRQIALDGRPVTIVGVAPKSYHGLSSILNVQAYLPFAMIA
                     TIENMPLADFNKQSNRNLQVYGRLRSGTTQQQADAALAVVARDLARAHPVDERKVELR
                     GFSLAAGRLTGSLDQDNSFATVSAIFLGLAALVLLLACVNVANLLLVRATVREREMVI
                     RSALGAARFRLIRQMLTESVLLSVLGGAGGIVLGLAGSRFLSSINLQTDLPVAFNFSF
                     DWHVLAFSAAVAVAAGAVVGIVPAVRLARANLNMVLREGGRGIAGRGHKLRDALVMIQ
                     VSAALTLLIVAGLFTRSLEQSEHAELGFNPNNVITMVMDPSEIGYNAERSLAFYKDLL
                     PRMRSLPGVASATAAQSIPMGLINNAEDTVTMEGYTPPPGQPAPQVRYNVIETDYFET
                     LQIPIAEGRAFRDSDDAKSPYVAIVSREMAKKYWPHGDPIGRHFTMASEPAHPMQVVG
                     VAADAHYGHPQRPPGAYFYTPFAQHFANTLLALEVRTQGDPGAMAPAIERAIHAQTPG
                     LPVFEVKTLHQALYSPNGLLLYQVVAGLAGVMGSLGMVLAIVGVYGVLSYVVSQKTGE
                     IGVRMALGAQRGDILRLIYRQGLWIVGIGLAVGLAASLGAAHLVRGMIAVSATDPVTY
                     VVISGTLAGIALLACYVPARRAMNVEPMRALRDE"
     gene            complement(2868..3194)
                     /locus_tag="VHE33_03560"
     CDS             complement(2868..3194)
                     /locus_tag="VHE33_03560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVT96556.1"
                     /translation="MLLGTLDMLILRTLVTGPAHGHTIAHVIEHTSEEVLQIEQGSLY
                     PALHRLEDRGWVSSYWGPSENNRKARFYKLTAAGRRRLTVETNRWESMVAAIGRVLGN
                     AGQAVE"
     gene            3436..4221
                     /locus_tag="VHE33_03565"
     CDS             3436..4221
                     /locus_tag="VHE33_03565"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HVT96557.1"
                     /translation="MLRTVRATRYVLPLREGGSLPAIVEADDLGLYVVKFRGAGQGIL
                     ALIAEIIAGEIGRVLGLRVPEIVLVDMDPALGRNEPDYEIRQLLKASAGLNVGLDYLP
                     GSTMFDPAARDAASSAEASLLVWFDAFVLNVDRTPRNANLLVWHRKLYPIDHGAALFF
                     HHDWTARESKIESPFMEIEHHILLPWANEIAEAAQAAHRLLTTAIVTEIVGLVPDAFF
                     AAIPDAAMAPAERSAGYIDFLVRRLAAAETFEQEAIRARSRLL"
     gene            4199..4585
                     /locus_tag="VHE33_03570"
     CDS             4199..4585
                     /locus_tag="VHE33_03570"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HVT96558.1"
                     /translation="MPDPASFDYAILRVVPRVERQEFINAAVIVFCPEKRYLDARVHL
                     DAARLRALWPDIDLELIRDHLDAVLRICQGEAGAGPIARLSQRERFHWLISPRSTIIQ
                     PSPVHTGVCEETEGLLDRLAKQFLLP"
     gene            4727..5116
                     /locus_tag="VHE33_03575"
     CDS             4727..5116
                     /locus_tag="VHE33_03575"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HVT96559.1"
                     /translation="MKRTPTLPRPTEGELELLRILWERGPSTVRDIHEQISLTRPLGY
                     TSVLKILQIMTDKGLVEREEAGKAHIYHPAASQNETQDQLLRDISERLFAGSAAQLAM
                     HALSLEPVNDHDLEALRTLIDQKRNRP"
     gene            5113..7413
                     /locus_tag="VHE33_03580"
     CDS             5113..7413
                     /locus_tag="VHE33_03580"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HVT96560.1"
                     /translation="MTHHWEALGWTLVHFLWQGAALALVYRVADMALARRSASARYVL
                     AVGALLGMLAVSAGTLAYEEAAFYQPPAQHADAATLKTVAAPATQPLHFDLTSTPAAA
                     PLRQADRFVLDHLMPYLDGLWLAGVLLLSLRTLGGWWLIRRMRARLQQAPHTLLLRLD
                     VLRRQMNIPRFVDLRLSTRITNPLTAGIFRPWILLPVTALTGLSAEQLEVVLAHELAH
                     IRRADYLWNLLQTLVETLLFFHPAVWWISRRVREERELCCDDIAVAACSDPTVYASAL
                     LRLEEERRTRLHLAMALDGHQSRAGLRARILRILGDREAAPRSFRPISLAGVSIALFL
                     FLCPLPKLFASFHEVPQVAARLAPAASRVAKTVMYHATHPVLQAATSAPTPSAASAAE
                     PRPTPAPASEDQTDSSAPTTGHTDYIDAMKAAGYDVDLDKYIAMKAQGITPQYAAEMS
                     KVFGEKLSADKLIGLKVQGVSPDYISKMRAAGFDVDANKFIAMRVQDITPEYAAEMAK
                     ATGETKLSADRLIAMRVQDIDPDYIAKMHAAGFDGDVNKLIAMKVQNITPEYADEMAK
                     ATGGNKLSVDTLIAMRVQDVTPDYIAKMHADGYDADVHKLIAMKVQDVTPEYADAMSK
                     LGFGKPSVDQLIAMKVQGVSADYAAKLHADGIEAASFNDLIQYRIFNVSPDFIAGMKA
                     AGFGDIPAKKLVTLRVQNVTPEFAKSVKAQFPDATLDDLVRMRVFNINGDFIASAKKH
                     GFEQLTIDKLVKLRISGILDDEESRK"
     gene            7461..>7683
                     /locus_tag="VHE33_03585"
     CDS             7461..>7683
                     /locus_tag="VHE33_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96561.1"
                     /translation="MSVRAAILTAILCLAGSRLFSAEDALQAIHGTCSIAPSTMPDHV
                     RYEFRHGDCENSDGDRYCHENDSDAPLTTF"
BASE COUNT         1434 a         2548 c         2231 g         1470 t
ORIGIN      
        1 gcgtccttcg ccgtttgcgc gaggggcgca atgccccctg ccagggcgaa cccaatgcaa
       61 caaacaaaca tctcccggag catgctcatg ctttttctac gtcccgaatg gaaccggagt
      121 tcccccaccg atcagacgca cgacccggca gctaagagag caacgtgctt cctgctccct
      181 actcatcccg cagcgcccgc atgggctcca cattcatcgc acgccgtgcc ggcacgtaac
      241 atgccagcag cgcaatcccc gccagcgttc ccgagataac gacataggtg accggatccg
      301 tcgcgctgac tgcgatcatg ccgcggacca ggtgagcggc gccaagactc gccgcgaggc
      361 ccaccgccag cccgatgccc acaatccaca aaccctgccg gtagatcagg cgcaaaatat
      421 cgccgcgctg cgctccgagg gccatgcgca ccccaatctc gcccgtcttc tggctcacca
      481 cataggacag cactccgtaa acgccgacga tggccagcac catgcccagg gatcccatga
      541 caccggccag gcccgccacg acctgataca gcaacaagcc atttggtgag tacagcgcct
      601 ggtgaagcgt tttcacttcg aacacgggca gtcccggggt ctgcgcgtga atggcgcgct
      661 cgattgcagg cgccatcgcc ccaggatctc cctgcgtccg cacttccagc gccaacagcg
      721 tgtttgcaaa atgctgtgca aacggtgtgt agaaatacgc gcccgggggc cgctgagggt
      781 gaccgtaatg cgcgtcggcc gcaactccca ccacctgcat cgggtgggcc ggctcgctcg
      841 ccatggtgaa gtgccggccg atgggatcgc cgtgcggcca gtacttcttt gccatctcgc
      901 ggctcacaat cgctacgtac gggctcttcg catcgtcgct gtcgcggaat gcccgtcctt
      961 ccgcaatcgg aatctgcaga gtctcaaaat aatccgtttc gatcacgttg taccggacct
     1021 ggggcgccgg ttgccctgga gggggcgtgt agccctccat ggttactgtg tcttcagcgt
     1081 tgttgatcag tcccattgga atggactggg ccgccgttgc cgacgccaca cccgggaggc
     1141 tgcgcatgcg cggcaggaga tctttgtaga acgccagcga gcgttccgcg ttgtaaccaa
     1201 tctcgctcgg atccatcacc atggtgatca cattgttcgg attgaagccc agttctgcgt
     1261 gctcgctttg ctcgaggctc cgcgtaaaca gccccgcgac aatcagcagc gtcagagctg
     1321 cgcttacctg aatcatcacc agtgcatcgc gcaatttgtg tccgcgcccg gcaatcccgc
     1381 ggccgccttc gcgcaggacc atgttcagat tcgcccgcgc cagccgcacc gccggaacaa
     1441 tgcccaccac tgctccggcc gcaaccgcaa ccgctgccga gaacgccagc acgtgccagt
     1501 cgaagctgaa gttgaaagca acgggcaaat ctgtttgcag gttgatcgag ctcaggaacc
     1561 ggctgccggc cagacccagc acaatgccac ccgctccgcc cagcacagaa aggaggacac
     1621 tctcggtaag catttgacgg atcagccgga accgggctgc gcccagtgcc gaacggatca
     1681 ccatctcccg ttcgcgtact gtagcgcgca ccagcagcag attggccacg ttcacgcatg
     1741 cgagcagcag caccagtgcg gccaggccaa ggaagatggc cgatactgtg gcgaaactgt
     1801 tgtcctggtc gagcgagccg gtcagccggc cagccgcgag cgaaaagcca cgtagttcca
     1861 ccttccgttc gtccaccgga tgcgctcgcg ccagatcgcg cgccactacc gccagcgccg
     1921 cgtcggcttg ttgctgtgtt gtgccgctgc gtaaacgccc gtagacctga aggttccgat
     1981 tgctttgctt attgaaatcg gccagcggca tattctcgat ggtcgcgatc atggcaaagg
     2041 gaagataagc ctgcacgttc aggatgctgc tgagaccgtg ataacttttt ggcgctactc
     2101 ccacgatcgt caccggcctg ccatcgagtg cgatctgccg cccgaccacg cccgcgtcgc
     2161 caccgaagtg gtctttccag tacgcgtaac tcagcaccac atacggatcg gcccctggag
     2221 ttgcgccttc ggtcggcttg aagaaccgtc ccagcgcagg cttcacaccc agcgcgtcga
     2281 agtagttgcc ggtcacgtag tcggtaaaga gacgatccgg tggattgccg gccgtggcga
     2341 tgccgtccag cccgtactgg tcggcgaata cctcggagaa gagaccgcga gtctgagccg
     2401 cgatcgtctg atactccgga accgaaaccg cgggctccca gctctcgccg ttctggcgga
     2461 gccccacttc gacgacgctg cgctcgttcc ggatatgcag agagctgaac agcaacccgt
     2521 tcaccatgct gaacacggct gtgttcacgc cgatgcccag cgccagtgtg acaatggcga
     2581 tcgccgcaaa gccggggctc ttccaaagtt gccgcaaggc ataccggaga tcgcgcccca
     2641 ggtcctcaat cgagacaaag ctgttcatct tccagatctc ctcgcgaatt gtggtggggt
     2701 tcccgagctt catgcaggct cgccgccgcg cctcttgctg tgacatgccg cgcgcaagat
     2761 tgtcggaggt ttcctgctcc agatgcgctt ccagctcaag cgcccgctcc tcgtcccact
     2821 tctgtcgatg ccaataccgg cgcaggctca tgtgctgcct cctggcccta ctcgactgcc
     2881 tggcccgcgt tcccgaggac tcgcccaatc gccgccacca tcgactccca acggtttgtt
     2941 tctactgtga ggcgcctgcg tcccgccgca gtcagcttgt agaaccgcgc cttgcggttg
     3001 ttctcgctgg gtccccagta tgacgagacc cagccccgat cctccagccg gtgcagcgca
     3061 ggatagagcg atccctgctc gatctgcagc acctcttccg acgtgtgctc gatgacgtgc
     3121 gcgatggtgt gcccgtgcgc cggaccggtc accagcgtgc gcaggatcag catgtccagc
     3181 gttcccagca gcaactcctc ttttttcggg cgggtcaggt tttcctccag atgtccggat
     3241 gagacttgcg gcagaacttc tatggggaat taccatacac cactcgaact tctatgggaa
     3301 agaaggagta cgtgacagcg tggcgccagg ttcccatttt ttgaaaggcg cgaattttgg
     3361 cacgctcgcc agagttgcca gttaagagct aaaagcttac ccgctgcctg taccctgtac
     3421 ccagtgacct gtccgatgct ccgtaccgtt cgcgcgaccc gctatgtgct tccgcttcgc
     3481 gagggcggct ctttgcccgc catcgtggaa gcggacgacc tcggtctcta cgtcgtcaaa
     3541 tttcgcggag cggggcaggg catccttgcg ctgatcgcag aaatcatcgc cggcgaaatc
     3601 ggccgcgtcc ttggcctgcg cgttccggaa attgtgcttg ttgatatgga tcctgccctt
     3661 ggccgcaacg agcctgacta tgaaattcgc cagttgctca aagccagcgc cgggctcaac
     3721 gttggcctgg actatctgcc tggctcgacc atgttcgatc ccgctgcgcg cgacgccgca
     3781 tcttcagcgg aggcctcgct gctggtatgg ttcgacgcgt tcgtgctcaa cgtcgaccgc
     3841 acgccgcgca acgcgaacct gctggtctgg caccgcaagc tgtaccccat cgatcacggc
     3901 gccgcgctgt tctttcatca cgactggacc gcgcgcgaga gcaaaataga atcgccgttt
     3961 atggaaatcg agcaccatat cctcttgccg tgggcgaacg aaattgcgga agctgcgcag
     4021 gctgcgcatc gattgctgac gacagcgatc gtgacggaga tcgtgggtct tgttccggac
     4081 gccttctttg cggcgattcc agatgcagcg atggcgcccg ccgagcggag cgccggctac
     4141 atcgatttcc ttgtccgccg cctggccgcc gcggaaactt tcgaacagga ggcgatccgt
     4201 gcccgatccc gcctcctttg attacgctat cctgcgcgtt gttccgcggg tggagcgaca
     4261 ggagttcatc aacgctgccg tgatcgtttt ttgtccggag aagcgctacc tcgatgcgcg
     4321 ggtgcatctg gacgccgccc gcctgcgcgc tctctggcct gacatcgacc tggaactgat
     4381 ccgcgatcat cttgatgctg tgcttcgaat ctgccagggc gaagccggcg ccgggcccat
     4441 cgctcgcctt tcgcagcgcg agcgattcca ctggctcatt tctcctcgtt cgaccatcat
     4501 tcaaccgtcg ccggtccaca ccggcgtgtg cgaggaaacc gaagggctgc tcgatcggct
     4561 ggcgaagcaa tttcttctgc cctgattccg ctcgctttat ttcgcctctt gcttacccgt
     4621 cggctctttc tccgggggct gccggcttcg tcccgttcgt cacaattttc ccacctacga
     4681 aaatcttcgt tgacatctac gaacctcttc gtatatggtt cggatcatga aacggactcc
     4741 gactcttccg cggcccaccg aaggcgaact agagctgctc cgcattctct gggagcgcgg
     4801 cccctcgacg gtgcgcgata ttcacgagca gatcagcctg acgcgtcctc ttggctacac
     4861 ttcggtgctc aagatcctgc aaatcatgac cgacaagggg ctggtggaac gcgaagaggc
     4921 cggcaaggcg cacatctatc atccggcggc ctcgcagaac gagacccagg accagctgct
     4981 gcgcgacatc tcggagcgcc tgtttgccgg ctcggcagcg caacttgcta tgcacgcgct
     5041 ctcgctcgag cccgtcaatg accacgacct cgaagcgctc cgaaccctca ttgaccaaaa
     5101 gaggaaccgt ccatgacaca ccactgggaa gctctcggct ggactctcgt tcacttcctc
     5161 tggcagggcg ccgcactcgc tcttgtctat cgcgttgccg acatggcgct ggcgcgccgc
     5221 tcggccagcg cgcgttacgt tcttgctgtg ggcgctctgc tcggcatgct ggccgtctca
     5281 gccggcactc ttgcctatga agaagccgcc ttttatcagc cgcccgcaca acacgcggat
     5341 gcagcgactc tgaagacggt cgcggccccc gcaacccagc cgctccactt cgatctcacc
     5401 agcaccccgg ccgccgcgcc gctgcgccag gcggatcgtt tcgtgctcga ccatctgatg
     5461 ccgtacctcg acgggctgtg gctggcgggc gttctccttc tctcgcttcg cacgctcggc
     5521 gggtggtggc tgatccggcg catgcgcgcg cgccttcaac aggctccgca cacgctcctg
     5581 ctccggctcg acgtgctgcg gcggcagatg aacataccgc gctttgtcga tctgcggttg
     5641 tccacgcgga tcaccaatcc gcttacggcc ggtatcttcc ggccgtggat tctgctgccg
     5701 gtgacggcgc tgacgggcct gagcgccgag cagctggaag tagtgctggc gcacgaactg
     5761 gcccacatcc gccgcgccga ctatctgtgg aacctgctgc agacgctggt cgagaccctt
     5821 ctcttcttcc acccggcggt atggtggatc agccggcgcg ttcgcgagga gcgcgagctg
     5881 tgctgcgacg acatcgcggt ggcggcctgc tcggatccga cggtgtatgc ctcggcgctg
     5941 ctgcgcctcg aagaagaacg ccggacgcgt ttgcatcttg caatggcact cgacggccac
     6001 cagtcgcgcg ccggactgcg cgcacgcatc cttcggatcc ttggcgaccg ggaagctgcg
     6061 ccgcgctcgt tccggcccat ttcgctcgcc ggagtgagca tcgcgctctt cctgttcctt
     6121 tgtccgctgc cgaagctgtt cgccagcttc cacgaggtgc cgcaggttgc cgcgcggctg
     6181 gcaccggcgg cgagccgggt cgcgaagacg gtgatgtatc acgcgactca tccggtactt
     6241 caggcggcga catccgcacc cacgccgagc gcggcttcgg ctgccgagcc gcggcctaca
     6301 ccggctccgg cgagcgaaga ccagacggat agttccgccc ccacgactgg ccataccgac
     6361 tatatcgacg cgatgaaagc ggccggttat gacgttgatc tcgacaaata cattgcaatg
     6421 aaggcgcagg gcatcacgcc gcagtacgcg gcggagatgt cgaaggtgtt cggcgaaaag
     6481 ttgtcggcgg acaagctcat tgggttgaag gtccaggggg tcagtcctga ctacatcagc
     6541 aagatgcgcg cggcgggttt cgacgtcgat gcgaataagt tcattgctat gcgcgtgcag
     6601 gacatcacac cggaatacgc ggcggagatg gccaaagcaa caggcgaaac caaattatcc
     6661 gccgatcgcc tgattgcgat gcgggtacag gacatcgacc cggactacat tgcgaagatg
     6721 cacgcggccg gtttcgacgg cgacgtcaac aagctgatcg cgatgaaggt acagaacatc
     6781 acgccggagt atgccgatga gatggccaaa gccacgggcg gcaacaagct ctcggtcgat
     6841 acgctcattg ctatgcgggt gcaggacgta acgcccgact acatcgcgaa gatgcatgcg
     6901 gacggatacg acgccgatgt tcataagctg atcgcgatga aggtacagga cgtgacgccc
     6961 gagtacgccg acgcgatgag caagctgggc ttcggcaaac ccagcgtcga tcagcttatt
     7021 gctatgaagg tgcagggagt cagcgccgac tacgccgcca aactccacgc cgacggcatc
     7081 gaggcggctt cgttcaacga cctcattcag taccgcatct tcaacgtttc gccggacttt
     7141 attgccggca tgaaagcagc cgggttcggg gatatcccgg ccaaaaagct ggtcacgctg
     7201 cgcgttcaga acgtgacgcc ggagttcgcg aagagcgtga aagcgcagtt ccccgacgcg
     7261 acgctggacg atctggtccg gatgcgcgtt ttcaacatca acggcgactt catcgcctcc
     7321 gccaaaaagc acggcttcga gcagctgacg atcgacaagc tggtgaagct gcgcatctcc
     7381 ggcatcctcg acgacgagga aagcaggaag tagacaggac ccgaaccgaa aaccggaccg
     7441 caacacccgg agattcgacc atgagcgtac gcgccgcgat tctcacagcc attctttgcc
     7501 ttgccggcag ccggctcttc tccgccgagg acgcgctgca ggccatccac ggcacctgct
     7561 cgatcgcgcc ctcgacgatg ccggaccacg ttcgctacga gttccggcac ggcgattgcg
     7621 agaacagcga tggcgaccgc tattgccatg agaacgactc ggatgcgccg ctgaccacct
     7681 tca
//
LOCUS       DASZYN010000137         1714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_581898, whole genome shotgun sequence.
ACCESSION   DASZYN010000137 DASZYN010000000
VERSION     DASZYN010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581898"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VHE33_03590"
     CDS             complement(<1..256)
                     /locus_tag="VHE33_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HVT96562.1"
                     /translation="MATTTAPALITLSAFTEAQARIQGVALHTPLVRLPIPGIDAAEV
                     YVKAEGLQPIGAFKIRGAYNKVAQLTPDERSRGVITYSSGN"
     gene            complement(300..737)
                     /locus_tag="VHE33_03595"
     CDS             complement(300..737)
                     /locus_tag="VHE33_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HVT96563.1"
                     /translation="MKAALDTNILAYAEGINDAAKRKTAWSLIERLPQDGVIPVQALG
                     ELFQVLVRKAGRTPAEARRAVYRWSDAFNLVETSTTILLAAADLATRQFNFWDAVIFS
                     AAAEANCRVLLSEDMQHDFSERGVTVVNPFAHPEHPLLEALLQ"
     gene            complement(734..985)
                     /locus_tag="VHE33_03600"
     CDS             complement(734..985)
                     /locus_tag="VHE33_03600"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HVT96564.1"
                     /translation="MPAEKAISATDANRRFSRILDGVRRGRTYLVTSHGQPVARISPV
                     DEPGQTVMDARKALLARLRGQKIINVGPWTRDELYEDKR"
     gene            complement(1016..>1714)
                     /locus_tag="VHE33_03605"
     CDS             complement(1016..>1714)
                     /locus_tag="VHE33_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVT96565.1"
                     /translation="TGKHTVAIETGIGNKLPPKLNEIYGAQQQLPRAFEAAQIRPEDI
                     DIVINSHLHFDHCGWNTTRTPDGAIVPTFLNARYFAHRGEVTHGRLQLERDAISYISD
                     NYDPLIASGQMTLLDTEPGETTLVVPGISVECFPGHTAQLLAVHIDADTNNPGCQRAT
                     YISDLIPTSAHLDITWVMGYDLDPVTCIAERKRFYSRAIPEKWLVLFTHDHHTPFARI
                     ELNEKGKPVIAKSV"
BASE COUNT          291 a          442 c          606 g          375 t
ORIGIN      
        1 gattgccgga ggagtaggta atgactcccc gggaacgctc gtcgggagtg agctgggcga
       61 ctttgttgta ggctccgcgg attttgaagg cgccgatggg ctgcaggcct tcggccttga
      121 cgtaaacttc tgcggcgtcg atgcccggga tcgggagccg gaccagcggc gtgtggaggg
      181 cgacgccctg gatgcgggcc tgggcttcag tgaaggcgga gagggtgatc agggccgggg
      241 cggtggtggt ggccatgact tattttagaa gggccgcggc cacgccgtta tttggagtct
      301 cactgcagga gtgcttcgag caagggatgc tctggatgcg caaacgggtt cacgacagtg
      361 acgcccctct ctgagaagtc gtgctgcata tcctcagaca acaaaaccct gcaattcgct
      421 tcggctgcag cgctaaagat gaccgcgtcc caaaagttga actgccgcgt ggcgagatcg
      481 gccgccgcca acagaattgt ggtggatgtt tcaaccagat tgaacgcatc gctccacctg
      541 taaactgccc ttcgggcttc agctggtgtg cggcccgcct ttcggacgag aacctgaaac
      601 agttcgccaa gcgcctggac tggaatgact ccatcctggg gaagcctttc gatgagactc
      661 caggccgtct tccttttggc ggcatcgttg atgccttcag cgtaggcgag gatattggta
      721 tcgagcgccg ctttcaccgc ttgtcctcgt agagctcgtc cctcgtccag gggccgacgt
      781 tgattatctt ttgcccgcgc agccgcgcca gcagcgcttt gcgcgcgtcc atgaccgtct
      841 ggcccggttc atcgactgga gagatcctgg ccacaggttg tccgtgactt gtaaccagat
      901 aggtgcgtcc tcgacggaca ccgtccagta tccgggagaa tcgacggttg gcgtcggtgg
      961 cggagatcgc tttttccgcg ggcatggctc ctccaagaag tagtgatatt cactactata
     1021 cgcttttggc gatcactggt tttcccttct cattgagttc gatccgcgcg aatggggtgt
     1081 ggtgatcgtg ggtgaagagg acgagccact tctccgggat agcgcgcgag tagaagcgct
     1141 tgcgctcggc gatgcaggtg accgggtcga ggtcgtagcc catgacccag gtgatgtcga
     1201 ggtgggcgct ggtggggatg aggtcggaga tgtaggtcgc gcgctggcag cccgggttgt
     1261 tggtgtcggc gtcgatgtgg acagcaagaa gctgcgccgt gtggccgggg aagcattcga
     1321 cagagatgcc ggggacgacg agggtggtct cgccgggttc cgtgtccagc agagtcattt
     1381 gccccgaggc gatgaggggg tcgtagttat cggagatgta gctgatggcg tcgcgctcga
     1441 gttgcaggcg gccgtgcgtg acttcgccac gatgcgcgaa gtagcgcgcg ttgaggaagg
     1501 tcgggacaat ggcaccgtct ggcgtgcggg tggtgttcca gccgcagtga tcgaagtgca
     1561 ggtgggagtt gatgacgatg tcgatgtctt cggggcgaat ctgtgcggcc tcgaaggcgc
     1621 gggggagttg ctgctgggct ccgtaaatct cgttgagttt gggcgggagc ttgttgccga
     1681 tgccggtctc gatggcgacg gtgtgctttc ccgt
//
LOCUS       DASZYN010000138         4992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_388290, whole genome shotgun sequence.
ACCESSION   DASZYN010000138 DASZYN010000000
VERSION     DASZYN010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388290"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /locus_tag="VHE33_03610"
     CDS             complement(<1..1400)
                     /locus_tag="VHE33_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HVT96566.1"
                     /translation="MSRTFQRHVVNALFGVLDYVAWPIGMVLVAPVMLSHLGAARFGV
                     WALAAAVVNAGSILASGFGDSNIQHVASQRGSENSDLLVRTVRSSMGIHLVLGLAMAL
                     LTWMLAPVAAHHVVQALPALAPDCLLSLRIASLIILLRAIETVCISTQRAFERYGPAV
                     FISIAGRLLSLLLAAVLAFLDRSIASILAATLVIIALSLILQMRNLRALLQAPSLLPL
                     WDRDALRALLAFGIFSWLQALASVVIGQADRLVAGVAMGAVAVAAYALCVQIAQPLYG
                     VVSSGLHFLFPHLAARRLTLSRRALRRMVLRALLANLVVVAAGTALLLMFGLRILRTL
                     AGAALAQSAAPVLPIIVWSTALLSLSVTAYYALMAFDRIRIVTFVNLAASAVMLASLV
                     WLIPQYHLFGIAMARFLYAFIALLLYLPLVLFLYPDLTIFKASRGLAPKSGEPSVPSH
                     ANVLGVRIEALNMSAAL"
     gene            complement(1517..2938)
                     /locus_tag="VHE33_03615"
     CDS             complement(1517..2938)
                     /locus_tag="VHE33_03615"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HVT96567.1"
                     /translation="MAGLPNIAALPSAPSIQPDSPSALPGTRPVGHRGSAAAHFTRAF
                     HDTSQPLSLSRQKRTREALLLTFIDPIPKECLRLWLLTRAEWQEALHWLDTAGLALYF
                     LGRLREENLDALLPDWVHARLSQNLADNKERTAALLAESRSLHAWFQRAGLSYATLKG
                     FSLDSHSVPALELRAQLDFDFLVAQSSAAEARRILEERGFALRGHHGRSWEFGAHDDE
                     SITIRDLYKPIPHRWVELHIEADASPLLQRAEMRRMHGTAMPVLDPVDLFLGQGLHIY
                     KHLRYEVTRPSHLLEFRRHVLARSSDDLFWRELRARAALDARHPVGLGIVILLIERLM
                     GPFAPPALTHWTVDNLPPAVCLWIETCAHAAILVDPPGTKLYLLLQEALASSGIPATR
                     TVRRALFPNIVPHSLRPLISRIVAHRPADSRSPAIVARGPRKGWRHHLCLMASRLRFH
                     LFEDLRYLRARHRFRKALSLIAS"
     gene            3173..4159
                     /locus_tag="VHE33_03620"
     CDS             3173..4159
                     /locus_tag="VHE33_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96568.1"
                     /translation="MLGTIRPAHAQDTPLLSGGVAFLTSTTGGNTGYSPIVEPLLAAP
                     IGPHLLLESRALLVENYHPDGKGGYDHTHFVGLTYMQGDYVVNPHLTVVGGSFILPFG
                     TYNERLSPVWINNFQDGPLDVQLGLLGTGTGVGGELRGSAARTARYSIDYTTWFSARS
                     GNQYFNAERSFGGRSSLYLPEQRLEMGLSYDRSLQGTHENFYGAHVWWEPKDEGLRLR
                     SEYARGHHAQGYWIEADYRSSAYGGLNSWVGRLEPLFRMQQTFRRDTIVSDGVPLVNT
                     QRADFGLDYNLPHNTRILTSYSRQFSSTGNENIWETGIVYRFLFPAWKGKVR"
     gene            4156..4470
                     /locus_tag="VHE33_03625"
     CDS             4156..4470
                     /locus_tag="VHE33_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVT96569.1"
                     /translation="MRAKNFVPGLLLAAAIGAASLAAPQGYTQSAPRRAQPQVRQYQA
                     AKTTQVEDEGQRVFEQNCSRCHATPEGFSSRISGTIVRHMRVRANLSEHDEQVLLKYF
                     NQ"
     gene            complement(4748..>4992)
                     /locus_tag="VHE33_03630"
     CDS             complement(4748..>4992)
                     /locus_tag="VHE33_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96570.1"
                     /translation="PEAQRSTAAAATMFTNSLVSSGATAAAGILFTQFGYPRILLGLA
                     ASAAIIALLCRLLLTPGKTRATAVPKLHPSAFGHVD"
BASE COUNT         1097 a         1266 c         1823 g          806 t
ORIGIN      
        1 agggcggcgg acatgttgag ggcttcgata cgtacaccga ggacgttggc gtgtgaggga
       61 acagacggtt ccccggattt cggagccagg ccgcggctag ctttaaagat ggtgaggtcc
      121 ggatagagaa agagcacaag cggcaaataa agaagcagcg cgatgaacgc atagagaaat
      181 cgcgccatgg caatgccaaa gaggtggtac tgagggatga gccaaaccag ggaggccagc
      241 atgaccgcac tggctgcaag attcacgaag gtgacgatgc gaatgcggtc aaacgccatg
      301 agcgcgtaat aggcggtgac gcttaacgac agaagcgctg tgctccagac gatgatcggg
      361 agtacgggcg cggcggattg agcgagggcg gcaccggcaa gtgtgcgaag gatgcgaagt
      421 ccgaacatga gaaggagtgc tgtgcccgct gcgaccacga cgaggttcgc caggagcgcg
      481 cggaggacca tgcgccggag ggcccggcgc gaaagagtga ggcggcgcgc ggcaagatgc
      541 ggaaagagga agtgcagtcc ggaagagacc acgccgtaga ggggctgcgc aatctggacg
      601 cagagcgcgt aggcggcgac ggcgactgca cccatggcga ctccggcgac caggcggtcg
      661 gcctggccga tgacgacgga ggcgagtgcc tgaagccagc tgaagatgcc gaacgccagg
      721 agcgcgcgga gggcatcgcg atcccagagg gggagcaggg agggagcctg gagcagggcg
      781 cgcaggttcc gcatctgcag aatgagggag agagcaatga tgacgagggt tgccgcgagg
      841 atcgacgcga tactgcggtc gaggaaagcg aggacagcgg caagcaggag cgaaagcagg
      901 cgtcctgcaa tgctgatgaa gacagcaggt ccatagcgct cgaaggcgcg ctgggtgctg
      961 atgcagacgg tttcgatggc gcgcagcagg atgataaggc tggcgatgcg gagcgaaagg
     1021 aggcaatcgg gagccaatgc cggcagcgcc tggacgacgt ggtgagccgc gacgggagcc
     1081 agcatccacg tgagcagggc catcgccagg ccgagcacga gatggatgcc catggagcta
     1141 cggacagtgc gcactagcag atcagaattc tctgagccac gctgcgaggc gacgtgctgg
     1201 atgttcgagt cgccaaagcc tgaggcgaga atcgatcccg cattcacaac cgcggcggcc
     1261 agggcccaca ctccgaagcg ggcggcgccg agatgactga gcatcaccgg tgcaaccagc
     1321 accattccaa tcggccaggc tacgtagtcg agaacgccga acagtgcatt gacgacatgt
     1381 ctctggaatg tgcgactcat ctctggaaaa tcaacgttaa cgggacgcga gacggggact
     1441 gtcagcagat tgttatgggt ttgtcatctg aaaaatgagg cgagcgaacg cggtcgagga
     1501 aactggagac aatccgctaa gacgcgatca gggagagagc cttgcggaaa cgatgtctcg
     1561 cgcgcaggta gcgcaggtcc tcgaaaagat ggaagcgcag gcggcttgcc atcaagcaca
     1621 gatggtgccg ccaacctttg cgggggcctc gggcgacgat cgcaggcgac cgcgaatcgg
     1681 ccgggcgatg cgcaacaatg cgggagatca gcgggcggag cgaatgtgga acgatgttgg
     1741 ggaagagcgc acggcggacg gtacgcgttg ccgggatgcc cgaggaagcg agggcttcct
     1801 gcagaagcag gtagagcttg gttccgggag gatcgacgag aatggcggcg tgcgcgcagg
     1861 tttcaatcca caggcaaacg gcggggggca gattgtcgac cgtccagtgg gtgagggcag
     1921 gcggagcgaa gggccccatc aggcgctcga tcagcaggat gacaatgccg agcccgacag
     1981 gatgacgcgc gtcgagggcg gcgcgcgcgc gtagttcgcg ccagaagagg tcatcgctgc
     2041 tgcgcgcaag gacgtggcgg cggaattcga gcaggtgcga ggggcgggtg acctcatagc
     2101 gaaggtgctt gtagatgtgg aggccctggc cgagaaagag gtcgacagga tcgaggacgg
     2161 gcatggccgt gccgtgcatg cggcgcattt cggctcgctg gagcagcggg gaagcatcgg
     2221 cttcaatatg cagttccacc caacgatggg ggattggctt gtaaagatcg cggatggtga
     2281 tggactcatc gtcgtgagcg ccgaactccc agctccggcc atggtgtccg cgcagtgcaa
     2341 agccgcgctc ctcgagaatg cggcgcgcct cggccgcact cgactgtgcg acgaggaagt
     2401 cgaagtccag ctgcgcgcgg agctccagcg cggggacgga gtgggaatcc agcgaaaagc
     2461 ctttgagggt cgcatagctc aggccggccc tctggaacca ggcgtggagg ctgcgcgatt
     2521 ccgcgagcag cgcagcggtt cgctccttgt tatcggccag gttttggctt agccgcgcgt
     2581 gcacccagtc ggggagaagg gcgtcgaggt tttcctcccg gagtcggccg aggaagtaga
     2641 gtgcgaggcc ggcggtgtcg agccaatgga gagcttcctg ccactcggcg cgggtgagca
     2701 gccagaggcg aagacattct tttggaatgg ggtcgataaa ggtgagcagc agggcttcgc
     2761 gtgtccgctt ctgacgcgaa agagacaggg gctgggatgt gtcgtgaaag gcgcgcgtga
     2821 aatgggccgc tgctgaaccg cgatggccga ctgggcgcgt cccgggtaga gcagaagggg
     2881 agtcgggttg gatgctgggc gccgagggca aagccgcgat attgggaaga ccagccatga
     2941 atgggactct agggcagaag cggcgaagcg ctgtcagatg tgtgcaaggg gaatgtcact
     3001 ggattgtcgt cggatccgga gacagggaag catgacaatc gtttgacaaa gtgcagacgg
     3061 tgacaggaca cgaagagccg tgggccggcc tagcttcgtg gttatgctgc ttcgttactc
     3121 ggattctcga cgactacgaa cgacgtctct ggctctcttc gcgatctgcg ctttgctggg
     3181 cacgatacgt cctgcgcatg cgcaggacac gccgctgctc tccggaggcg tggcgttcct
     3241 caccagcacg acgggaggca ataccggcta ttcgccgatc gtggagccgc tgctggcggc
     3301 gccgatcgga ccgcatctgc tgcttgagtc gcgagcgctg ctggtggaga actatcaccc
     3361 tgacggcaag ggtggctacg atcacacgca ctttgtcggg ctgacttata tgcagggtga
     3421 ctacgtggtg aacccccatc tgacagtagt gggcggcagt ttcatcctgc ctttcggcac
     3481 ctacaacgag cggctgtcgc cggtatggat caacaacttt caggatggtc cgctggatgt
     3541 gcaactgggg ctgctgggga cgggcacagg tgtaggggga gaactgcgcg ggtcggcggc
     3601 tcgcacggcg aggtattcga tcgactacac gacgtggttc tcggcgcgga gcggaaacca
     3661 atacttcaat gcggaacggt ctttcggcgg ccggtcgagc ctgtatttac cggagcagag
     3721 gctggagatg ggtttgtcat acgaccgctc gctgcagggc acgcacgaga atttctatgg
     3781 agcgcatgtg tggtgggaac ccaaggacga gggattgcgg ctgcgatcgg agtacgcgcg
     3841 cggacaccat gcgcagggat attggatcga ggccgattac cgaagcagcg cgtatggcgg
     3901 cctgaacagc tgggtcggac ggctggagcc gctcttccgg atgcagcaga cgtttcggcg
     3961 ggacacgata gtgagcgatg gagtaccgct ggtgaacacg cagcgagcag attttgggct
     4021 cgactataac ctgccgcaca acacacggat cctgaccagc tattcgcggc agttttcttc
     4081 gacgggcaac gaaaacatct gggaaacggg gatcgtgtac cgcttcctgt ttccggcatg
     4141 gaaggggaag gttcgatgag agcaaagaac tttgttccag gattgttgtt agcggcggcg
     4201 attggggcag cgagtctcgc tgctccgcag ggatacacac agtccgcgcc gcgtagggcg
     4261 caaccgcagg tgaggcaata tcaagctgcg aaaactaccc aggtggagga cgaggggcag
     4321 cgcgtgtttg agcagaactg ttcgcggtgc cacgcgacgc cggaggggtt ctcgtcgcgg
     4381 atctcaggga cgattgtgcg gcacatgcgg gtgcgcgcaa acctgagcga gcatgatgag
     4441 caggtgctgc tgaaatattt caatcagtag agcggcggac agcgatgagc gtatagcgcg
     4501 tcgcggtgag tccggtggat tcgggaatcc ttcgggaatc ccgaaacaca aattacaaca
     4561 ccaggggcag caaggctgca gaggtcgaac ctgactctgc ggccttttta tcttcggcgg
     4621 caggcaacag caaaaaagga agaagcttcg ggttggggat ctgaatccca ggggaaaagt
     4681 tgaggcatga tgggccccga gaagaactct gagcggccgc catttgtcct tttgaggtta
     4741 ggttggacta atcgacatgg ccaaaggcgg aaggatgaag cttgggcacg gcagttgcgc
     4801 gcgtcttgcc cggcgtgagc agaagccgac acaagagggc gatgatggct gcggatgcgg
     4861 cgaggccgag cagaatgcgg ggatatccaa actgcgtaaa gagaatgccg gcggcggccg
     4921 tggctccaga gctcacaagg gaatttgtga acatggtggc ggcggccgcg gtactgcgtt
     4981 gagcttcagg ag
//
LOCUS       DASZYN010000139        13468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_262891, whole genome shotgun sequence.
ACCESSION   DASZYN010000139 DASZYN010000000
VERSION     DASZYN010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 13468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13468
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262891"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VHE33_03635"
     CDS             <1..782
                     /locus_tag="VHE33_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVT96571.1"
                     /translation="IVVLGSGTSMGVPTLGCQCAVCTSTDPRNRRTRPSIAVTWNNSG
                     ATHLVVIDTGPDFRSQALREGIRHVDAVFYTHAHADHILGLDDLRPLSFAYKPKSLPL
                     YADRPTADILRRVYDYTFSPTSTYPNKARVELRDLAEEQTVNVHGAQFQRVPLLHGDL
                     EVAGFRFGSAAYLTDMNAIPESSLLLLNGLDVMIIDALRPQPHPSHASIAESLRWVER
                     VRPRQAWFTHMSHEVEHDTTEQTLPANVRLAWDGLRIPFEL"
     gene            786..1763
                     /gene="ribF"
                     /locus_tag="VHE33_03640"
     CDS             786..1763
                     /gene="ribF"
                     /locus_tag="VHE33_03640"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HVT96572.1"
                     /translation="MQVYRSIPELPKPMPPTVAAIGNFDGVHRGHQEIIARVRDRARS
                     LGAQAIAVTFDPHPLVLLRPDQAPKLITPIPKRLRLLAETGLDATLVIPFTREFSLQT
                     PCEFAERILCDALHVVEVHEGDTFRFGHDAAAGITELRELGAELNFEVVSHPSLRIRG
                     ITVSSSEVRRRIDAGHIALARALLGRPFSILSTPVHGRGVGTKLSTPTINLAPYPELL
                     APDGVYVTRLRIGEKSGAPWLDAVTNAGSRPTFDGAGFAVESHLLDGPPPVELSDSTP
                     IEVCFLMRLRGERRFPSPDALKAQILRDVTRAREYFRRSADSFARRSTE"
     gene            complement(1903..4149)
                     /locus_tag="VHE33_03645"
     CDS             complement(1903..4149)
                     /locus_tag="VHE33_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HVT96573.1"
                     /translation="MNLITPSDWDESPPPPRRRPRRRDSDFRPPSAHRKIAGSLALAF
                     LVVVAAAAGCLTGLTLVYSVDLPQIDELEHYRPSTTTELYDTHGRIFGSFALQRRVAV
                     NYDDFAPILRHAVISTEDRDFESHWGIDLVRVAGAAWHDLSSKNRAQGASTLTMQLAR
                     NLFLTNQRTWSRKLQEVFLTLQIEHAFTKEQIFTLYANQIYLGSGVYGFEAGAEYYFS
                     KHASQLILPEAALLAGLPKGPSEYSPINNPDRAFRRRNVVIDSMLEDGKITAAEAESA
                     RSSPLGLHVEAPPNSVAPWFVEEVRRELERRFGSEEVHAAGLRVYTTLDLDLQHAANA
                     AVLSGLGSYERSRGWHGNLLNVLSAGLNVDAFRHPDWSLPAKTGTWIHALATGILPYQ
                     VTGRVGDRQFILTPEDWAWTGFHTADQFLKPGDIIYLKVTGEEGGILHATLEQDSGVQ
                     GSLMAMNNATGDVLALVGGRDFNLSQFDRATQAERQTGSSFKPYVYTAAVEDGASPQE
                     TILDRPVSFGTYVPRNYDGKFKGRISLLEAFADSRNIPALELADRVGVRKVIATAHRF
                     GITTNIPPYLPIALGSAEVTLEQQVDAYSVFPNDGIRVAPRLVKRVESADGITLPSDG
                     QDISDVISEKTARTMMVFLRGVTSEGTAAKVAALNHPLGGKTGTTNDFTDAWFVGFSP
                     SITCGVWVGYDDRKSLGEKQTGAAVALPLWTDFMRTAITASPNESFPGDGPRPARPPL
                     QQTAALDH"
     gene            complement(4207..5403)
                     /locus_tag="VHE33_03650"
     CDS             complement(4207..5403)
                     /locus_tag="VHE33_03650"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HVT96574.1"
                     /translation="MSRSLNHTLLGVSAAIILVVVFGGLGPAGVSASQSDGAYRQMGV
                     YEEVLHKIQTDYVTDPNIQNVTTGALHGLLESLDSDSSYLTAAEYANYVQHEHEGVAQ
                     VGMDVSKRYGYGSVISVIPGSPADKAHIEDGDLIEAIGGHSTREMSVAMIRLMLEGKP
                     GTSVTFELVRHFKPEPDEVTLTRAAVAPPALFQQQYENSSILYLKPVVLTKDRVDEIV
                     ARLREMQKGGNHKVLLDLRDVAEGDPQQGIRLANAFLQSGTIASLQGQTVPVQTFSAE
                     SAKFVTAAPLVILVNHGTAGAAEIVAAAILDDKRGDVVGERTFGEGGQQKTFPMPDGS
                     ALLLTVAKYESPSGKKIEDVAVTPNVAVSQSVEEAPAENESNPQEPRTDDQLNRALQV
                     LKQKSA"
     gene            complement(5509..6762)
                     /locus_tag="VHE33_03655"
     CDS             complement(5509..6762)
                     /locus_tag="VHE33_03655"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT96575.1"
                     /translation="MALSDSRESVRMAMDTLRSNKLRSGLTILGVVIGVATVITISSL
                     INGVNNRVRDMAKSFGTNVLWLYRWNPIGVRPTAEMLARKYFTTDDCKALETLPHVLG
                     VNCGRQFANWQLGVGYVAVKYKDKKIQNTILEGDQPSFATVDDVQFAEGRPFTDDEAF
                     RASNVTVIGHDTAEKLFGTEDPIGKDIQVNGDVFTVIGVLDKYKQAFGSGDNPNDNKA
                     VFPLTTFKKIHPEVLDTWVSVKYDDPANKSIVTEEVRTLLRERRRVHVEKDDDFAIFS
                     PDSILDFWSQITAGLVIFMFAVSSVGLLVGGVGVMNIMLVSVTERTREIGVRKAIGAT
                     KRNVLVQFTFEAMTLCSLGGLIGIFLGTMLTMAIRYGISFPASLSTFWVITGFVVSAA
                     IGLVFGIYPAWKAATLDPIEALRYE"
     gene            complement(6766..7992)
                     /locus_tag="VHE33_03660"
     CDS             complement(6766..7992)
                     /locus_tag="VHE33_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT96576.1"
                     /translation="MQFGEAIKVALQSLWANKMRTILTILGVIIGVASVIAVITLVNG
                     ANMFVVKKVARKGSDTLTVTRLPPVIFTGEEYLKVNKRKNIEYDDFLYLQRTCSRCQV
                     LAAQLSSVGKIVSGKQSSDNTNINGWTSNMADISDQNIVLGRPLTPTDDSMGTHDIVV
                     GADVVDNVLPPGDPIGRELRVDGEVYSVVGVVERQGSMFGQSQDNFVNMPLSTYLHTH
                     GQNHSITISAKAGMAPGALDAAIDQVRALMRSRRHDRPGDDDTFTISTNETFISLWKQ
                     ISETFATVIVGIAAISLVVGGVVIMNIMLVSVSERTREIGIRKALGAKRKDVMLQFLI
                     ESAAMALLGGLFGVIGGVTVAEIVTHLVGFPSAIALWSVFLGLFVATTVGIFFGVYPA
                     HKAAQLDPIVALRAEL"
     gene            complement(8103..9059)
                     /locus_tag="VHE33_03665"
     CDS             complement(8103..9059)
                     /locus_tag="VHE33_03665"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT96577.1"
                     /translation="MSRYLVTGCAGFIGSWITQALVERGETVRGLDNFETGKRDNLAH
                     LAGRFEFVECDLRDAEGVARACEGVDFILHQAALPSVPRSVKDPRTSHTANIDGTFNL
                     LEGARAAGVKRIVYAASSSAYGNQPGFPRVETMFPMPIAPYPVQKLACEFYMKSYWQV
                     YGIETVCLRYFNIFGPRQVPDSPYSGVMAKLTLQMMRGERPTINGDGEQGRDFTYIEN
                     AVSANLLALDGPAEKVAGRVFNVACGERHTINETFRILAELLDFKEQPIYGPPREGDI
                     RDSLADISAAREAFGYVPKVGFEEGLRRTAAWYCEQYASARA"
     gene            9139..10083
                     /locus_tag="VHE33_03670"
     CDS             9139..10083
                     /locus_tag="VHE33_03670"
                     /inference="COORDINATES: protein motif:HMM:NF042587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HVT96578.1"
                     /translation="MIAPFRLAPYFRTRPWGFEDLHPWYDYKTDGDPIGEVWLTGEMC
                     LADTGPMTGKSLAVITKQHGDALLGQGLNGPDFPLLIKLLFPKDKLSVQVHPDDTLAQ
                     HYGEPRGKTECWYALDAKPGASVALGIRPGVTLEQVKSAVQDTTLESLLEMVPVQTGD
                     MIFVDAGTVHAMGPGLVILETQQSSDLTYRMYDYGSARELHLDKSFAAMRLKTRAGKV
                     PPHPVNGHLVLIDERYFRVERWPLQGQAANALTARSPVVQMLFVKQGSIEISGQGFES
                     FRVERNQLAVIPAATPQWTIAGTESTELIRTLPAVELV"
     gene            10108..11232
                     /locus_tag="VHE33_03675"
     CDS             10108..11232
                     /locus_tag="VHE33_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HVT96579.1"
                     /translation="MAIDFRPIILAGGSGTRFWPRSRRARAKQVLALDGQRTMIQRTL
                     DRLTATDSLEPLAKPHDVWVITNAHLSDAICTQLDCVPGKQVICEPAARNTAPAAGLA
                     AFLIERLCPDAVLGIFPSDHVITDEKAFVAIIRRAIGLAAAGDNIVVLGIQPTRPETG
                     YGYIETGEARQDGVLCVRRFTEKPNQEKAEEFLAAGNYFWNSGMFVWSAKTLANAVRE
                     LMPDTAPLLQQIVAAWGSPDFERVFAELYPKCENISVDYAILEPRSARGEHHSNLYCI
                     PSKFGWSDLGSWTALHEYQVEQKAADEHTNVLDAQGSVVLDSTGNYVFSPKKLVALVG
                     VQDLVVVETDDALLITTRAHSQDVGKVVKELAGRGQEKLI"
     gene            11235..12668
                     /locus_tag="VHE33_03680"
     CDS             11235..12668
                     /locus_tag="VHE33_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HVT96580.1"
                     /translation="MSTKSPVKFGTDGWRGVIADDFTFENVHAAAAAIAGYVLTKEDP
                     KKGVCIGYDTRFGSPAFAAAVAEVIAGAGIPVQLADRITPTPALSFAVKHRAAAGGVM
                     ITSSHNPAQWNGVKYKASYGGSGSPSIMADIESLLGKPLPANSPAKIEKVNFVPPYVE
                     AIEKFVDLDLIAKSGFRFAIDCMYGAGAGILAGIFSKIGVDFIEIRANHDPLFPGINP
                     EPIEPHVRACQDIVVEKKCDAGLITDGDADRIGAADENGNFVDPHKIYSVLLQWILER
                     KGWPGDVTRAFNTTSMLDRIAARYGRKLHEHGIGFKFVCDLMLTENILIGGEESGGIG
                     IQRHLPERDGLLNALLLANVMAEEKKTLGQLVERLQQEYGEHQYGRIDLHIPDSLKNA
                     TIARAKAGVKEFAGMPVEKVETLDGIKFWLDIPEAKSKQNAAKTWLLLRASGTEPLLR
                     IYSESCSKESVKKLLEAAREFAMSSGG"
     gene            12709..13116
                     /locus_tag="VHE33_03685"
     CDS             12709..13116
                     /locus_tag="VHE33_03685"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVT96581.1"
                     /translation="MVATYNGGSMAGSVADYPYETRLNILHKPLELIDEKAIADACTY
                     KWFNQTLCQVNGSVVRMAVIEGEYHWHQHTDDDEFFYVVEGTLLIDLEDRTVELNPRQ
                     GFVVPRGTTHRTRATQRTVILMVENAGIIPTGN"
     gene            13194..>13468
                     /locus_tag="VHE33_03690"
     CDS             13194..>13468
                     /locus_tag="VHE33_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96582.1"
                     /translation="MSTTATAVQRPLTSLPAWKALQAHYEKIRTQHLRELFRTDAQRG
                     TRLTVEAEGIYLDYSKHRITDETVKLLLDLAEQSGLRERIEAMFSGE"
BASE COUNT         2732 a         4277 c         3834 g         2625 t
ORIGIN      
        1 aaatcgttgt tctcggcagc gggacctcca tgggggtgcc gacgctcggc tgccagtgcg
       61 ccgtatgcac ctctaccgat ccacgcaatc gcagaacccg cccctcgatt gccgtcacct
      121 ggaacaatag cggtgcgacg catttggtgg tcattgacac gggtccggac ttccgcagcc
      181 aggcattgcg cgaaggcatc cggcacgtgg atgccgtgtt ctatacgcac gcccatgccg
      241 accatatcct cggtctcgac gatctgcgtc ccctgagctt cgcctacaag cccaaatccc
      301 tgcccctcta tgcggatcgg ccaacagccg atattctccg gcgggtctat gactatacct
      361 tctcgcccac ctcgacgtat cccaataagg cgcgcgttga gctgcgcgat ctggcagagg
      421 aacagacagt caatgtgcac ggggcgcaat ttcaacgagt gccgctcctg cacggtgatc
      481 tcgaggtcgc ggggtttcgc tttggatcgg cggcctacct taccgatatg aacgccattc
      541 ccgagtccag tttgctgctg cttaacgggc ttgacgtaat gatcatcgat gccctccggc
      601 cccagcccca tcccagccat gccagcattg cggagtcgct gcgatgggtc gagcgcgttc
      661 gcccccggca ggcctggttc acccacatgt cgcacgaagt ggagcacgac accaccgagc
      721 agacacttcc agccaatgtg cggctcgcct gggacggctt gcgcattcct tttgagctat
      781 aagcgatgca ggtctaccgt tccatcccgg agttgcctaa accgatgcct cccaccgtgg
      841 cggccatcgg caacttcgat ggcgtgcacc gaggccacca ggagatcatc gcccgcgtcc
      901 gggatcgggc tcgaagcctc ggagcacaag ccatcgctgt caccttcgat ccgcaccctc
      961 tggtgctgct gcgcccggac caagctccga agttgatcac tcccatccca aagcggttgc
     1021 gcttgctcgc cgaaaccggc cttgacgcca cactcgtcat tccctttacg cgggagtttt
     1081 cgttgcaaac gccatgcgag tttgccgaga ggattctttg tgacgctttg cacgtcgttg
     1141 aagtacatga aggcgatacc ttccgcttcg gacatgacgc ggcggccggc atcaccgaac
     1201 tgcgtgagtt aggggccgag ctgaactttg aggtcgtctc ccacccctcc ctcaggatcc
     1261 gcggcatcac ggtctcgagc agcgaagtgc gacgccgcat cgatgcgggc catatcgcat
     1321 tggctcgggc tttgctgggt cgtccatttt cgatcctctc aacgcctgta cacggacggg
     1381 gggtcggcac aaaactatca accccaacga tcaatctcgc tccgtacccg gagctgctgg
     1441 cgccggatgg tgtttatgta actcggcttc ggatcggcga gaaatccggg gcaccgtggc
     1501 tcgatgcggt gacgaatgca ggttcacggc ccaccttcga cggcgcaggc ttcgcggtgg
     1561 aatcacacct gctcgatggc cctccgccag tcgaactcag cgattccact ccgatcgagg
     1621 tgtgcttcct gatgcgcctt cgtggcgaac gtcgcttccc ttctccggat gctctgaagg
     1681 cgcaaatcct gcgcgatgtg acgcgcgctc gcgagtattt ccgcaggtcg gcggactcgt
     1741 tcgcccgccg cagcacggag tgattcctgg cctgttagga ttggcggatg tttaaactcg
     1801 gcaggccgga gaatttgggc cagtggcttg ttcacatcgt attgggcatc attgcgctgg
     1861 cactgatcgc ctggatggtg cgggtcttca ttctctgacg tttcagtgat ccagcgcagc
     1921 ggtttgctgc agaggcgggc gtgctggcct gggtccgtct cccggaaagc tctcgttggg
     1981 cgaggcggtg atcgccgttc tcatgaaatc cgtccacaaa ggcaaagcga cggccgcgcc
     2041 cgtttgtttc tcaccaagtg atttgcggtc atcgtagccg acccagacgc cgcacgtaat
     2101 cgagggtgaa aaaccaacga accaggcgtc ggtaaagtca ttggtggttc cggtttttcc
     2161 tcccagcgga tgattcagcg cagcaacttt tgctgcggtt ccctcgctgg tgacaccgcg
     2221 aagaaagacc atcatggtgc gagcggtctt ctcgctgatc acatcactga tgtcctggcc
     2281 gtcactcggg agcgtgatgc cgtccgcgct ctcgacccgc ttgaccagcc gcggcgcaac
     2341 ccgaatgcca tcgttgggaa aaacggaata cgcatccacc tgctgctcaa gggttacctc
     2401 ggccgacccc agagcaatcg gcagatacgg cgggatgttg gtggtgatcc caaagcgatg
     2461 cgccgtggcg atgactttgc gcacaccaac ccgatcggcg agctcgagag cagggatatt
     2521 ccgtgaatcg gcaaacgcct ccagcagcga gattcggcct ttgaacttgc catcataatt
     2581 tcgcggaacg taggtaccga acgagactgg ccgatcgagg atcgtctcct gaggcgacgc
     2641 gccgtcttcc acggccgccg tgtaaacata gggcttgaag gaagagccgg tctggcgttc
     2701 tgcctgcgta gcgcggtcga actgggagag attaaagtcc cggccgccca ccagcgccag
     2761 tacatcgcct gtggcgttat tcatggccat cagcgaaccc tgaacaccgc tgtcctgctc
     2821 gagggtggcg tgaaggattc cgccctcctc gccagtaact ttaaggtaga tgatgtcacc
     2881 cggcttcagg aactgatccg ctgtgtggaa gcccgtccac gcccagtcct ccggcgtaag
     2941 aatgaactgc cggtccccga ctcggcccgt cacctggtac ggcaaaatac cggtagcgag
     3001 cgcatggatc cacgtccccg ttttggctgg cagggaccag tccgggtgcc tgaatgcgtc
     3061 gacgttcagg ccggcggaga ggacgttcag cagattgcca tgccagccgc ggctgcgctc
     3121 gtatgagcct agcccgctga gaaccgcggc gttggccgca tgctgtagat cgaggtcgag
     3181 cgtggtgtaa accctgaggc ctgcggcgtg aacctcctcg gaaccgaacc ggcgctccag
     3241 ttcccgccga acttcttcaa caaaccacgg cgcgacgctg ttcggaggcg cctcgacgtg
     3301 caggcccaga ggtgacgagc gagcggattc cgcttcagcg gcggtgatct tgccatcctc
     3361 gagcatcgag tcaataacga cgttgcgccg ccggaacgcg cggtccggat tgttgatcgg
     3421 tgaatattcc gacggaccct taggcagacc ggcgagcagc gccgcctccg gcaaaatcag
     3481 ctgactcgcg tgcttcgaga agtagtattc tgcgccggcc tcaaacccgt agacgccgct
     3541 acccaggtag atctgattgg cgtagagggt gaaaatctgc tccttggtaa atgcatgctc
     3601 gatctgcagc gtcagaaaga cctcctgcag cttgcgggac caggtgcgct gattggtcag
     3661 gaagaggttg cgcgccagct gcatggtgag cgtcgaggct ccctgcgccc ggttcttgga
     3721 agaaaggtcg tgccacgcag ccccagcgac gcgaacgaga tcgatgcccc aatggctttc
     3781 gaagtcgcga tcctcggtgg agatgacggc atgccgcaga atcggcgcaa agtcgtcgta
     3841 gtttacggcc acgcggcgct ggagcgcaaa agatccgaaa atgcgcccgt gcgtgtcgta
     3901 aagttctgtc gtcgtgctgg gccgataatg ctccagctca tcgatctggg gcagatccac
     3961 agaatagaca agagtcagcc cagtgaggca gccggccgct gcagccacga ccaccagaaa
     4021 tgccagagcg aggctgcccg caatcttgcg atgtgccgaa gggggacgga aatcggagtc
     4081 gcgtcgccga ggccgccggc gtggaggcgg gggactctca tcccagtccg atggcgtgat
     4141 caggttcaac gtgccatccg cttcctttgg tctggcacca tccgagaaat ggccctggcc
     4201 tgcccttcag gcgctctttt gtttcaggac ctgcagcgcc cggttcagct gatcgtccgt
     4261 acgcggttcc tgcggattgc tttcgttctc cgcaggcgcc tcttcaacac tctggcttac
     4321 cgcaacgttg ggagtcaccg ccacgtcttc gatcttcttg cccgaagggg actcgtactt
     4381 ggccaccgtt agcagcagcg cggatccgtc aggcatcggg aaggtcttct gctgcccccc
     4441 ttcgccaaag gtacgctcgc caaccacatc gccgcgtttg tcatcgagga tcgcagccgc
     4501 gacgatttca gcggcccctg ccgtgccatg gttgacgaga atcaccagag gagcggcggt
     4561 cacgaacttc gcggattccg cgctgaacgt ctgcacaggt acggtttgtc cttgcagcga
     4621 cgcgatcgtc cccgactgaa ggaatgcatt cgccagccgg attccctgct gcggatcgcc
     4681 ctcggcgaca tcgcgcaggt caagcagaac cttgtggtta ccgcccttct gcatctcccg
     4741 cagtcgcgca acgatctcgt cgacgcgatc tttggtcagc acgaccggct tcagataaag
     4801 aatgctggag ttctcatact gctgctggaa cagcgctggc ggcgctaccg ccgcacgcgt
     4861 cagcgtaacc tcgtcaggct cgggtttgaa gtggcgaacc agctcgaaag tgacactcgt
     4921 gcccggcttg ccttccagca tcaggcgaat catcgccact gacatctcgc gcgtggagtg
     4981 tccgccgata gcctcaatca gatcgccgtc ctcgatatgc gccttgtcgg ccgggctgcc
     5041 ggggatcacc gagatgacgc tgccgtatcc gtaacgcttg gagacatcca tccccacctg
     5101 cgccacgcct tcgtgctcat gctgcacata gttggcgtat tcggccgcgg tcagatagct
     5161 cgaatccgag tcgagagatt ccagcaggcc gtgcaacgcg cctgtcgtca cattctgtat
     5221 gttcggatcg gtgacgtagt cggtttggat cttatgcagc acttcctcat agacgcccat
     5281 ctgccggtaa gcgccgtcgc tctgactggc actaacacca gccgggccca gcccgccgaa
     5341 gacgacaaca aggatgatgg ctgcagaaac gccaagcaga gtgtgattaa gacttctgga
     5401 catagggttc aaagctgggg gtacaccacc gctacctgaa tgatacccca cccggcagcg
     5461 agcacgatgg ccgctttggg gcaggcgtat ctggcccggc agcggctttc actcgtaccg
     5521 cagggcctca atcggatcca gcgtcgccgc tttccacgcc ggatagatgc cgaacacaag
     5581 gccgatcgcc gccgagacga caaaccctgt aatcacccag aacgtggaca ggctcgccgg
     5641 aaatgaaatg ccatagcgaa tggccatcgt cagcatggtg cccagaaaga tgccaatcag
     5701 ccctcccagc gaacacaacg tcatggcttc gaacgtgaat tgcaccagaa cgttgcgttt
     5761 cgttgcacca atggccttgc gaaccccaat ttcgcgcgtg cgctcggtga ccgacaccag
     5821 catgatattc atcacgccca cgccacccac caacaagccg acactcgaca ccgcaaacat
     5881 aaaaatcacc agaccggccg tgatctgcga ccagaagtcg aggatggagt cagggctaaa
     5941 gatggcgaaa tcgtcatcct tttcaacgtg cacacggcgc cgctcgcgca acagggtgcg
     6001 cacctcttcg gtgacgattg acttgttggc cggatcgtcg tatttcaccg aaacccaggt
     6061 atccagtact tccgggtgaa tcttcttgaa cgttgtcagc ggaaagacgg ctttattgtc
     6121 attgggattg tcgccgctgc cgaaggcctg cttgtacttg tcgagcacac cgatcaccgt
     6181 gaagacatcc ccgttaacct ggatgtcttt tccgataggg tcttccgtgc cgaagagctt
     6241 ttccgcagta tcgtgcccaa tcaccgtcac attggaggcg cggaacgctt cgtcgtcggt
     6301 aaacggccgg ccctcggcga actgcacgtc atctactgtt gcgaaggacg gctgatcgcc
     6361 ttcaagaatc gtgttctgga tctttttgtc cttgtacttg accgcgacgt agcccacccc
     6421 gagctgccag ttggcaaact gacgcccgca gttcaccccg agaacgtgcg gcaacgtttc
     6481 cagcgccttg cagtcatcgg tcgtaaagta tttgcgcgcc agcatctcag cggtcgggcg
     6541 gacgccgatg gggttccagc gataaagcca cagcacattg gtgccgaagg acttggccat
     6601 gtcccgaacg cggttgttca cgccgttgat cagcgacgag atcgtgatca ccgtcgccac
     6661 tccaatcacc acgccgagga tggtcagacc ggagcgcagt ttgttgctgc gcagcgtatc
     6721 cattgccatc cggaccgatt cacgggaatc actgagtgcc atggtttata gctccgcgcg
     6781 cagggcgacg atgggatcca gttgagccgc tttatgagcc ggataaacgc caaagaagat
     6841 gccgacagta gtggctacaa agaggcccag gaagaccgac cacagcgcaa tggcagaggg
     6901 gaagcccacg aggtgggtga cgatttcagc tactgtgacg cctccgataa cgccgaacaa
     6961 accgcccagc agcgccatcg cagccgattc gatcaggaac tgcagcatca catctttgcg
     7021 cttggcgccg agggccttgc gaatgccgat ttcgcgagtc cgctccgaca ccgaaaccag
     7081 catgatgttc atgatcacca cgccgccgac gaccagcgaa atcgcagcga tcccaacgat
     7141 caccgtggcg aacgtctcgc tgatttgctt ccacaagcta atgaaggttt cgttggtcga
     7201 aatcgtgaag gtgtcgtcat cacccggacg atcgtggcgg cgggaacgca tcagagcgcg
     7261 cacctgatcg attgctgcat cgagcgcacc tggagccatc cccgctttcg ccgagatagt
     7321 aatgctgtgg ttctgcccat gcgtatgcag ataggtgctc aagggcatat tgacgaagtt
     7381 gtcctggctc tgtccaaaca tcgagccctg gcgttccacc accccgacca cgctgtaaac
     7441 ttcgccatcc acgcgcagtt cccgcccaat tggatccccg ggaggcagca cgttatcgac
     7501 aacgtctgcg ccaacgacga tgtcgtgcgt tcccatggag tcgtctgtcg gcgttagcgg
     7561 ccgtcccagc acgatgttct ggtctgaaat gtccgccatg ttcgaggtcc agccgttgat
     7621 gttcgtgttg tcgctggatt gcttgccact gacgattttg cccacagacg aaagctgcgc
     7681 cgccagcacc tggcagcgcg agcaggtccg ctgaagatag agaaagtcgt cgtattcaat
     7741 gtttttccgc ttgttgacct tcaggtattc ctcgccggtg aagatcacag gaggcaggcg
     7801 ggtcacagtc agagtgtccg atcccttacg ggcgaccttc ttgaccacga acatgttcgc
     7861 gccgttgacc agcgtaatga cggcgatgac ggaggccacc ccgatgatca cgccaaggat
     7921 cgtcaggatg gtgcgcatct tgttagccca aagcgattgc agcgcgacct taatggcttc
     7981 gccaaattgc atgaccggcg gttgtccctc ggccccggct tcggtgcaga cagcaatatt
     8041 ctccggggac tctgcgccta gtttacgcag aattcagacg ggatgttccc tctttgtgac
     8101 ctttaggccc ttgccgaagc gtactgttcg caataccagg ctgccgtccg gcgcagaccc
     8161 tcttcgaaac ccaccttcgg tacatacccg aaggcttcgc gagccgccga aatgtcagcc
     8221 agagaatccc gaatatcgcc ctcgcgcggc ggcccataga tcggctgctc cttgaagtcc
     8281 agcaactcag ccaggatgcg aaaggtctcg ttgatcgtgt gccgttcgcc acaggccacg
     8341 ttgaaaacgc gcccagccac cttctccgcc ggcccatcca gagccagcag gttcgccgag
     8401 accgcattct cgatgtaggt gaagtcccgc ccctgttcgc cgtctccgtt aatggtggga
     8461 cgttcgccgc gcatcatctg cagcgtcaac ttcgccatca cgccgctgta tggagaatcg
     8521 ggcacctgac gcggaccgaa gatgttgaag taacgcaggc acaccgtctc gataccgtaa
     8581 acctgccagt agctcttcat gtagaactcg caggccagtt tctgcaccgg ataaggcgca
     8641 atcggcatgg gaaacatcgt ctcgacgcga ggaaaccccg gctgattacc gtaggcggac
     8701 gacgaagccg catagacgat ccgcttcacg cccgccgcac gtgccccttc gaggagattg
     8761 aaggtgccat cgatgttggc cgtgtggctc gtgcgtgggt ccttcaccga ccgcggcacc
     8821 gagggcagcg ctgcctggtg caggatgaag tccacgccct cgcaggcgcg cgccacgcct
     8881 tcggcgtcgc gcaaatcgca ttcgacaaac tcaaaacggc ctgcgagatg ggcgaggttg
     8941 tcccgttttc ccgtctcaaa gttatccagc ccgcgaaccg tttcgccgcg ctccaccaga
     9001 gcctgcgtaa tccacgatcc aataaacccc gcacaaccgg tcacaagata gcgcgacatg
     9061 gttttcatcg tagcggatgc accaggcgag cgggatcaca acctttaggg gtctgcggca
     9121 tactaaaggg tcatggaaat gatcgcgccc tttcgtctgg cgccctactt tcgcacgcgt
     9181 ccctggggat tcgaggacct ccatccctgg tacgactaca agaccgatgg agaccccatc
     9241 ggcgaagtct ggctcacggg cgaaatgtgc cttgccgaca ccggccctat gacaggcaaa
     9301 agccttgccg tgatcacgaa acagcacggc gatgccctcc tgggccaggg tttgaacggc
     9361 cccgattttc cgctgctgat caagctgctc ttccccaaag acaaactcag cgtccaggtc
     9421 catcccgacg atacgctggc gcaacactac ggcgagcccc ggggaaagac cgaatgctgg
     9481 tatgcgctcg acgccaagcc gggagcatct gtggcgctgg gcatccgtcc cggtgtgacc
     9541 ctggagcagg tgaaatccgc ggtgcaggat acaacgctgg agtcgctgct ggagatggtc
     9601 cccgttcaaa ccggcgacat gatcttcgtc gacgccggaa cggttcacgc catgggcccg
     9661 gggctggtga ttctggaaac ccagcagtcc agcgacctga cctaccgcat gtacgactac
     9721 ggcagcgcgc gggagctgca cctcgacaaa tcgtttgccg cgatgcgcct gaagacgcgt
     9781 gcgggcaagg tcccgccgca cccggtcaac ggccacctcg tgctcatcga cgagcgttac
     9841 tttcgcgttg aacgctggcc gctgcagggt caggccgcca atgcgctcac ggcgcgaagt
     9901 cccgtcgtac agatgctgtt tgtcaagcag ggatcgattg agatttcggg tcagggcttt
     9961 gaatccttcc gcgtggaacg caaccagctc gccgtgatcc ccgccgcaac gccgcaatgg
    10021 acaattgcgg gaactgaaag caccgagctg attcgcacgc tgccggctgt cgaactcgtt
    10081 taggattgcg ttttgtctca ggattccatg gcgatcgatt ttcgacccat cattcttgct
    10141 ggcggcagcg gtacccgttt ctggccccgc agccggcgtg ctcgtgccaa gcaggtcctg
    10201 gccctggatg gtcagcgcac catgatccag cgcaccctgg atcggctgac ggccaccgac
    10261 agcctggaac ctctggccaa gccacatgac gtctgggtca ttaccaacgc gcacctttcg
    10321 gacgcgatct gcacccagct cgattgcgtc ccgggaaaac aggtcatctg cgaacccgct
    10381 gcccgcaaca ccgccccggc cgcgggcctc gctgcctttc tgatcgagcg cctctgtccc
    10441 gatgccgtcc ttggaatttt cccttccgat catgtgatca ccgacgagaa ggcatttgtc
    10501 gcgatcattc gtcgtgccat cggcctcgca gcagccggag acaacatcgt ggtgctcggc
    10561 atccagccaa cgcggcctga aaccggctat ggatacatcg agaccggtga ggctcgacag
    10621 gatggtgtcc tgtgcgtccg gcggttcacc gagaagccca accaggagaa ggccgaggaa
    10681 tttctcgcgg caggaaatta cttctggaac agtggcatgt tcgtgtggag cgcgaagacc
    10741 ctggcgaacg ccgtgcgcga actcatgccg gataccgcac cactcctcca gcaaatcgtc
    10801 gccgcctggg gttctcccga cttcgagcgg gtttttgcgg aactgtatcc aaagtgcgag
    10861 aacatcagcg tcgactacgc gattctcgaa ccgcgctccg cacgcggcga acaccattcg
    10921 aacctgtact gcatcccatc gaagttcggc tggagcgatc tcggctcctg gaccgcgctc
    10981 cacgaatatc aggtcgagca gaaggcggct gacgagcata cgaatgtcct ggacgcacag
    11041 ggcagcgttg tgctggattc caccggtaac tacgtattca gccccaaaaa gctggtcgcc
    11101 ctcgtcggcg tgcaggacct tgtggtcgtg gagaccgacg atgctctcct gattaccacc
    11161 cgggcgcact cccaggatgt cggcaaggtg gtcaaagaac tcgccggccg cgggcaggaa
    11221 aaactgatct gatcatgtcg acaaaatctc ccgtcaaatt cggtaccgat ggatggcgcg
    11281 gcgtcatcgc cgacgacttc acctttgaga acgttcacgc cgcggcggcc gcaatcgccg
    11341 gctacgttct gaccaaagaa gatccgaaga aaggcgtgtg tattggatac gacactcgct
    11401 tcggttcgcc tgcctttgca gcggctgtgg ctgaagtcat cgctggagca ggcattcccg
    11461 tgcagctggc cgaccgcatc acccccaccc cagcgctttc cttcgccgtg aagcatcgtg
    11521 ccgccgccgg tggcgtcatg attacctcca gccacaaccc ggcacaatgg aacggcgtca
    11581 agtacaaggc ctcctacggc ggctccggca gcccgtccat catggctgac atcgaaagcc
    11641 tgctgggtaa gcccctgcct gccaactctc cggcaaaaat cgagaaagtg aacttcgtac
    11701 cgccttatgt tgaggccatc gagaagttcg ttgatctcga cctgatcgcg aaatcgggat
    11761 tccgattcgc catcgactgc atgtacggcg cgggcgctgg aattctcgct ggaatctttt
    11821 cgaaaatcgg cgtcgacttt atcgaaatcc gtgccaacca cgatcccctg ttccccggca
    11881 tcaatcccga gccgatcgag ccgcatgtcc gcgcctgtca ggacatcgtc gtcgagaaaa
    11941 agtgtgacgc gggcctgatt accgatggcg acgcggaccg catcggtgca gccgatgaaa
    12001 acggcaactt cgtcgatccc cacaagattt actccgtgct gctgcagtgg atcctcgagc
    12061 ggaagggctg gccgggcgac gtcacgcgcg ccttcaatac gacatccatg ctcgatcgga
    12121 tcgccgcgcg ctatggccgc aaactgcacg agcacggcat cggcttcaag tttgtctgcg
    12181 acctcatgct caccgaaaac atcctgatcg gcggcgagga gtcgggcggc atcggcatcc
    12241 agcggcacct ccccgaacgc gatgggctgc tcaatgcgct tctgctcgcc aacgtcatgg
    12301 ctgaagaaaa gaagaccctg ggccagctcg tcgagcgcct gcagcaggag tacggcgaac
    12361 accagtacgg ccgcatcgat ctgcacatcc ctgactcgct gaagaacgca accatcgccc
    12421 gtgcgaaagc cggcgtgaaa gagttcgcgg gcatgcccgt ggagaaagtc gagaccctcg
    12481 acggcatcaa gttctggctc gacatccctg aagcaaagag caaacagaac gccgcaaaaa
    12541 cctggctgct cctgcgtgcg tccggaaccg aacctctcct ccgcatctac tccgaatcct
    12601 gctcgaagga atcggtgaaa aagctgctgg aggctgcccg cgagttcgcg atgagcagcg
    12661 gcggctgatc ggcgtcctgt ttcctcccat tcacacgacc gtcgtaccat ggttgcgacg
    12721 tacaacggag gctccatggc cggttcggtc gccgactacc cctacgaaac gcgcctcaac
    12781 attctgcaca agcctctgga actcatcgac gaaaaagcca tcgccgatgc ctgcacctac
    12841 aaatggttca accagacgct ctgccaggtc aacggcagcg tcgtgcgcat ggccgtcatc
    12901 gagggcgaat accactggca tcagcacacc gacgacgatg aattcttcta tgtcgtcgaa
    12961 ggcacgctcc tcatcgacct tgaagatcgc accgtcgaac tgaacccacg ccagggattc
    13021 gttgttccca gaggcacgac ccaccgcacc cgcgccaccc aaagaaccgt catcctcatg
    13081 gtcgaaaacg ccggaatcat cccaaccggg aattgacatc gaactgcgct cgcgcgcgag
    13141 accatccggc atccacctga atcctatagt tgagacttct tccggagaac atcatgagca
    13201 ccaccgcgac cgccgttcaa cgcccgctga cttccctgcc cgcctggaaa gcccttcaag
    13261 cccattacga aaagatccgc acccaacacc tgcgcgaatt gttccgaacc gacgcccagc
    13321 gcggtacgcg actcaccgtc gaagccgaag gcatctatct cgactactca aagcaccgca
    13381 tcaccgacga gacggtgaag cttctcctcg atctcgccga gcagagcggg ctccgcgagc
    13441 gcatcgaggc catgttctcc ggtgaaaa
//
LOCUS       DASZYN010000140         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1226920, whole genome shotgun sequence.
ACCESSION   DASZYN010000140 DASZYN010000000
VERSION     DASZYN010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226920"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VHE33_03695"
     CDS             complement(<1..432)
                     /locus_tag="VHE33_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96583.1"
                     /translation="MTVTIDNHLTGWQQVRGWEKEYPEFALPLFSKSRFIAFGCDWTD
                     KSNRMGMRMRVTCDDRQSVRDIHLAAMTLLSYWPAAFLQAGEGTAVYYKRILQFFSSV
                     RVQPSAEEASEHYVHLSADVGWEKQEFVEVLRQTWGYMSDLP"
     gene            660..1967
                     /locus_tag="VHE33_03700"
     CDS             660..1967
                     /locus_tag="VHE33_03700"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVT96584.1"
                     /translation="MCLVTSFVVFLQPLAVVMTCPSFGNAMTLLAGWVFAPRRTVTGM
                     IAAAGAVGRKHHSTFHRLFASARWSLDNVGLVVFDLITPWLSDSIMLAADDTLARKRG
                     LKMFGVGMHHDPLLSSRGKAITNWGHSWVVLGVILRFPLWPDRAFCLPILFRLYLNKK
                     SAARCARVYRTRPELLVEMLTWLCQVRKTRRFHLIADSAYGGQSVLNHLPVNCDLTSR
                     LVMDARLYGPAPARVPGRNGRPRKRGPQLPTPAEMLQQRAARVSLTIYGRQDRVRMCD
                     AVGSVYAAPDRLLRIVAVEPLVGGRTKQTFYSTHHEATAIEVLTWYAWRWAIEVAFHD
                     SKQSLGFEEPQSWTRRAVQRTAPVAMLLYSLIVLWFVHEGHQHYRPLTRPWYTTKAEP
                     SFADMLGTLRRCSLREQVSSWGLGGQGSRKVLDLLENTAALAA"
     gene            complement(1983..>2451)
                     /locus_tag="VHE33_03705"
     CDS             complement(1983..>2451)
                     /locus_tag="VHE33_03705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HVT96585.1"
                     /translation="LEFSKVIAEGERVLDVGTGGGVPGVVLQIVRDDLRVTLADSVGK
                     KARAVEDIVRALGLSCPVHHGRAEELLAREKYQSLLIRAVAPLVKLLNWFAPFWGSFE
                     RLLVVKGPAWVDERHTAREAGALNGLQLRKVGTWPLPGTHSESVLLEVKPRGT"
BASE COUNT          441 a          810 c          709 g          491 t
ORIGIN      
        1 cggcagatca ctcatatagc cccacgtttg acgcagaacc tcgacaaatt cctgtttctc
       61 ccagccgacg tccgcgctca aatgcacgta gtgctcgctt gcctcctcgg cgctcggctg
      121 cacgcggacg ctcgagaaga actgcaggat ccgcttgtag tacacagctg tgccctcgcc
      181 ggcctgaaga aatgccgccg gccagtaaga caacagcgtc atcgcggcca ggtggatgtc
      241 ccgtacgctc tgccgatcat cgcaggtcac ccgcatgcgc atgcccatgc gattactctt
      301 gtcggtccaa tcacagccga aggcgatgaa tcttgacttg ctaaaaagcg gcaaggcgaa
      361 ctccgggtac tctttctccc agccgcgcac ttgttgccag ccggtcagat ggttgtcgat
      421 cgtcacagtc acgaggccac catccacggc ccgccaggcg tcggcccacg ggcaatcgtg
      481 cggagcatcg tcaaagaact ttcgcatgaa ctttgtctcg gcctcggcgt ctgcttcatc
      541 ccccttagtt cggctttcgc agtttttgct agcgttgaca gcttcttgag aaataggacg
      601 agccccgttc acgatgcaga taggttctca cgccaatcgc atcgccaagg aggctcgcca
      661 tgtgtcttgt tacttcgttc gtcgttttcc tgcaaccttt ggccgtggtc atgacctgtc
      721 cttcgttcgg caacgcgatg acgctgctgg ccggttgggt cttcgcgccg cgacggactg
      781 tcactggcat gatcgcggca gcgggtgctg tcggccggaa acatcactct acgtttcacc
      841 ggctgttcgc cagcgctcgt tggtcgctgg acaacgtcgg cttggtggtc ttcgacctga
      901 taacgccctg gctgagcgac tcgattatgt tggcggccga cgacacactg gcccgcaagc
      961 gcggcttgaa gatgttcggc gtaggcatgc accacgaccc gctgctctcc agccgcggca
     1021 aagcgattac caactggggt cacagttggg tcgtgctggg cgtcatcctc cgctttcccc
     1081 tttggcccga tcgggccttc tgtctgccga tcttgttccg tttgtatctg aacaagaaat
     1141 cggctgcccg ctgtgcccgc gtctatcgca cgcgtcccga gttgctggtc gagatgctca
     1201 cgtggttgtg ccaggtccgc aaaacaaggc gttttcatct gatcgccgac agcgcctacg
     1261 gcggccagag cgtgcttaac cacctgccgg tcaactgcga cctgaccagt cggctggtca
     1321 tggacgcgcg actctatggg cctgcgcctg cgcgcgtgcc gggtcggaat ggtcgcccac
     1381 gcaaacgcgg acctcagttg cctacgcccg ccgagatgct ccagcagcgt gccgcacgcg
     1441 tcagcttgac gatctatggc cgccaggatc gcgtgcggat gtgcgacgcg gtcggcagcg
     1501 tgtacgccgc acccgatcgt ctgttgcgaa ttgtcgcggt cgagcccctg gtaggcggtc
     1561 gcacgaagca aaccttttat tcgacccacc acgaagcgac cgccatcgaa gtgttgacct
     1621 ggtatgcctg gcggtgggct atcgaagtcg cgttccacga cagcaaacag tcgctcggct
     1681 tcgaggagcc gcagagttgg acacgccgcg ccgtccaacg caccgcaccg gtggctatgc
     1741 tgctgtacag cctgatcgtg ctgtggttcg tgcatgaagg acatcaacat taccgtccgc
     1801 tcacccggcc gtggtacacc acgaaagcgg aaccttcgtt cgccgatatg ctgggcacct
     1861 tgcgacgatg cagcctccgc gaacaagttt cctcatgggg actcggcggc caaggtagcc
     1921 gaaaagtcct cgacctcctg gaaaacacgg ccgctctggc ggcctaaact gcgaaactcg
     1981 aactaagtgc cgcggggttt cacctcgagc agcacgcttt ccgaatgggt gcccggtagc
     2041 ggccaggtgc cgaccttgcg caattgcaga ccgttcagcg cgccggcttc gcgcgccgtg
     2101 tgacgctcgt cgacccaggc cgggcccttc acgaccagca gccgctcgaa cgatccccag
     2161 aacggtgcga accaattcag aagcttcacg agcggcgcca ccgctcgtat cagcagcgac
     2221 tggtacttct ctcgggcgag caattcctcg gcccgaccgt ggtgcacagg gcacgatagc
     2281 cccagcgcgc gcacgatgtc ctcgacagcc cgggcttttt tgccgacgga atctgccagg
     2341 gtcacgcgca gatcgtcgcg cacgatctgt agcaccacgc cagggacgcc accgccggtg
     2401 cccacgtcaa gcacccgctc cccctcggcg atgaccttgg aaaattcgag c
//
LOCUS       DASZYN010000141         4186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_6376, whole genome shotgun sequence.
ACCESSION   DASZYN010000141 DASZYN010000000
VERSION     DASZYN010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6376"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1696
                     /locus_tag="VHE33_03710"
     CDS             <1..1696
                     /locus_tag="VHE33_03710"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HVT96586.1"
                     /translation="EVVYANYCRPEDFAKLKDLSVDVHGKIVLCRYGGNFRGVKVYVA
                     QEKGAAGVLIYSDPADDGYYRGDMYPHGPYRPATAVQRGSVQFMFKYPGDALTPGVAS
                     KPDLPASERLPLDKVTSLPKIPSEPLSYHDASPILEHLGGPDAPREWQGALPFTYHVG
                     PGVKVHMNIQMDYEYATIWDVIGAVPGAEFPDEWVVAGNHRDAWVYGAVDPNSGTAAM
                     LEAVHGVGALLKTGWKPKRRIVFCSWDAEEWGLIGSTEWAEDHANQLAHAVAYFNTDV
                     GVSGPNFDASAVPSLKEFVRNVTKAVPSPKGGTVYEQWKKEQADASARRHLGVQEHAS
                     QEAANVDHDVRVGDLGSGSDFTPFIQHLGVPSTDIGSGGPYGVYHSVFDNYAWFTMNA
                     DPTFVYEQEMARVFGLEVLHMADADVLPYDYVTYGKAISAYLDAAQNKAGEAGMKGFD
                     FAQAKAAAEKFTELAEKVRAIQAAASGSPEQLKTLNAQLRDVEEGFLSAQGLPNRPWF
                     KHTIYAPGEYTGYAAVVIPGVNEAIDAKDAARAQAQMTVLTGAIGRASSTLEAIAK"
     gene            complement(1790..2560)
                     /locus_tag="VHE33_03715"
     CDS             complement(1790..2560)
                     /locus_tag="VHE33_03715"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HVT96587.1"
                     /translation="MQTNPLDFTGKVALVTGAASGMGLATAHAFAEAGAAVLMADARE
                     DALRQEAHKLSAAGHKAAAVRCDVSDEAEVEHMVQRAVSEFGRLDAAFNNAGVMAHIA
                     PTGESTREEWDRVININLRGVWCCMKYELRQMERQGSGAIVNNASVGALTGNPGIGSY
                     IASKHGVIGLTRTAALEYVKKGIRVNAINPGLIDTQIARDVVAGNEDAYHEIEKQVPI
                     GRAGRPEEIASAVLWLCSPGASYVIGHGLTVDGGMTVV"
     gene            2790..3821
                     /gene="tdh"
                     /locus_tag="VHE33_03720"
     CDS             2790..3821
                     /gene="tdh"
                     /locus_tag="VHE33_03720"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF003808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HVT96588.1"
                     /translation="MRALVKARAERGLWLEDVPEPDYGINDVLIRVRRTGICGTDVHI
                     YQWDEWAQQTIPVPMVIGHEFVGEIAAVGSNVSDFHVGDLVSGEGHVTCGRCRNCLAG
                     RRHLCAYTSGVGVNRPGAFAEYITLPMTNIWRHHAGVSEEIAAIFDPFGNAVHTALAF
                     PVLGEDVLISGAGPIGLMAIPIVRHAGARYVVATDLNPFRLDLARKMGATLAVDPRKT
                     PVAEVQKQLGMQEGFDVGLEMSGSPVAFAEMLNNMSHGARIAVLGIPSKPMETDWRKI
                     IFNMLTVRGIYGREMYETWYKMSVLVTSGVDISPVITHRFSYQEYEKGFEAMIGGNCG
                     KVILDWTTI"
     gene            complement(3822..>4186)
                     /locus_tag="VHE33_03725"
     CDS             complement(3822..>4186)
                     /locus_tag="VHE33_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HVT96589.1"
                     /translation="DKVILTFPRFTMTQQFELSGTLSAMGMPQAFTPAADFSGMTGRP
                     DFSISAAIHKAFIDVNEQGTEAAAATSTVMVATAMHMARPEPPPIVFNADHPFLFMIR
                     ETKTGAILFLGRVDDPRQ"
BASE COUNT          760 a         1124 c         1517 g          785 t
ORIGIN      
        1 cgaggtggtg tacgcgaact actgccggcc ggaagacttt gcaaagctga aggacctgag
       61 cgtcgatgtg catggaaaga tcgtgctgtg ccgttatggc gggaacttcc gcggcgtcaa
      121 ggtctacgtt gcgcaggaga aaggcgcggc gggcgtgctg atctattccg acccagccga
      181 cgacggctac taccgcggcg atatgtatcc gcatgggccg tatcgtccgg cgaccgccgt
      241 gcagcgcggg tcggtgcagt tcatgttcaa gtatccggga gatgcgctga cgccgggggt
      301 ggcgtcgaag cccgatctgc cggcgtcgga gcggctgccg ctggacaagg tgacgagcct
      361 tccgaagatt ccgtccgagc cgctgagcta tcacgatgct tcgccgattc tggagcatct
      421 gggcgggccg gacgcgccgc gcgagtggca gggcgcattg ccgttcacct atcacgtggg
      481 accgggcgtg aaggtccaca tgaatatcca gatggactac gagtacgcga cgatctggga
      541 cgtgatcggc gcggtgccgg gcgcggagtt tcccgatgag tgggtggtgg cggggaatca
      601 tcgcgacgcg tgggtctatg gcgcggtcga tcccaacagc ggcacggcgg cgatgctgga
      661 agcggtgcac ggcgtgggcg cgctgctcaa gacaggatgg aagccgaagc ggcggatcgt
      721 gttctgctcg tgggacgcgg aggagtgggg gctgatcgga tcgacggaat gggcggagga
      781 tcacgccaat cagctcgcgc atgcggtggc ctacttcaac acggatgtgg gcgtgtcggg
      841 gccgaacttc gatgcatccg cggtgccgtc gctgaaggag ttcgtgcgca acgtgacgaa
      901 ggccgtgccg agcccgaagg gcgggacggt ttacgagcag tggaagaagg agcaggccga
      961 cgcctcggcg cgccgtcacc tgggggtgca ggaacacgcc agccaggagg ccgccaatgt
     1021 ggatcacgat gtgcgcgtgg gcgatcttgg atcgggttcg gacttcacgc cgttcatcca
     1081 gcacctggga gttccgtcga ccgacatcgg ttcgggcggg ccgtatggcg tgtaccactc
     1141 ggtgttcgat aactatgcgt ggttcacgat gaatgccgat ccgacgtttg tctatgagca
     1201 ggagatggcg cgggtgtttg gcctcgaagt gctgcacatg gccgacgccg atgttctccc
     1261 ctacgactac gtgacgtatg gcaaggcgat ttccgcgtat ctggatgccg cgcaaaacaa
     1321 ggcaggggag gccggcatga agggcttcga ctttgcgcag gcgaaggcgg cggcggagaa
     1381 gttcactgag ctggcggaga aagtgcgagc gatccaggcg gcggcttcgg gttcgccgga
     1441 acaattgaag acgctgaacg cgcagcttcg cgatgtggag gaagggtttt tgtcggcgca
     1501 ggggctgccg aaccggccgt ggttcaagca cacgatctat gcgccgggcg agtacacggg
     1561 atacgcggcg gtggtgattc ctggggtcaa tgaggcgatc gacgcgaagg atgcagcccg
     1621 cgcgcaggcg cagatgacgg tgctgaccgg ggccatcggg cgggcgtcgt cgacgctgga
     1681 ggcgattgcg aaatagggag cgcgcgaatt caggcgcgaa gcaaatccgg gccggatcgc
     1741 aaatgaggct cagggattcg ggacgagact tttttggcat gaggagcgat cagaccacgg
     1801 tcattccgcc atccacggtt agtccgtggc cgatcacgta gcttgccccg ggactacaaa
     1861 gccacagcac ggccgatgcg atctcctccg gtcttcctgc acggccaatg gggacctgct
     1921 tttcgatttc gtggtaggcg tcttcgttcc cggcgacaac atcgcgggca atctgtgtat
     1981 cgatgaggcc ggggttgatc gcatttacgc gaatgccttt cttcacgtac tcgagagctg
     2041 cggtgcgggt aagcccgatg acgccgtgct tggaggctat gtaagagcca ataccagggt
     2101 tgccggtcaa tgctcccacc gacgcgttgt tcacaatggc gccgctgccc tggcgttcca
     2161 tttgccggag ttcgtacttc atgcagcacc acacaccacg caaattgata ttgatgaccc
     2221 gatcccattc ttcgcgcgtg ctttccccgg tgggagcgat gtgggccatg acgcccgcat
     2281 tattaaacgc cgcatccagg cgaccgaact cggagactgc acgctgcacc atgtgctcga
     2341 cctcagcctc atcgctcaca tcgcagcgga cggcggcggc tttgtgaccg gcggcactca
     2401 gcttgtgcgc ctcctggcga agggcgtctt cacgagcgtc cgccatcagg accgcagcgc
     2461 ccgcctcagc gaatgcgtgg gcggtggcga gtcccatgcc tgaagcggct cccgttacga
     2521 gggcgacctt tccggtgaaa tcgagaggat tggtttgcat gactatgctc cacgaggaga
     2581 tgtggggtga gcagcgggtc tttgcgatgg tactgagacg aagttcgttg tgtcgagcag
     2641 cggtcgatgt ggaatcctgt gttggcgcgc atgggcggga tgaagtggaa tagcttaacc
     2701 tcagagaggt gattccccgc tgtgcgcgat ttgaaagtgc agcgcaggga aggaaggaca
     2761 ggcccgaggc ctcgtccggc aggagatgca tgcgagcact ggtgaaggcc cgcgcggaac
     2821 gcgggttatg gcttgaggat gtgccggagc cggattacgg catcaacgat gtgctgatac
     2881 gggtgcggcg gacgggcatt tgcggcaccg acgtccacat ttatcagtgg gacgagtggg
     2941 cgcagcagac gatcccggtg ccgatggtga tcgggcacga gtttgtcggg gaaattgccg
     3001 ccgtcgggtc gaatgtgtcg gactttcatg tcggggatct ggtctccggc gaaggccacg
     3061 tgacgtgcgg gcggtgccgc aactgtctcg cggggcgtcg tcatctgtgc gcctacacgt
     3121 cgggcgtggg cgtgaatcgt ccgggcgcgt ttgcggaata catcacgctg ccaatgacga
     3181 atatctggcg gcatcacgcg ggggtgtcgg aagagatcgc ggcgatcttc gatccgtttg
     3241 ggaatgcggt gcacacggcg ctggcgtttc cggtgctggg cgaggacgtg ctgatctcgg
     3301 gcgcgggacc gattggtctg atggcgattc cgatcgtgcg ccacgcgggc gcgcgctatg
     3361 tggtggcgac ggatctgaat ccgttccgtc tggacctggc gcggaagatg ggcgcgaccc
     3421 tggccgtcga tccgcgcaag acgccggtgg cggaggtgca gaagcagctg gggatgcagg
     3481 aagggttcga tgtcggcctc gagatgtcgg gctcgccggt ggcgttcgcc gagatgctca
     3541 acaacatgag ccacggcgcg cggatcgcgg tgctggggat tccgtcgaag ccgatggaga
     3601 cggactggcg gaagatcatc ttcaacatgc tgacggtgcg cggcatctac gggcgcgaga
     3661 tgtacgagac ctggtacaag atgtcggtgc tggtcacgtc gggggtggac atttcgccgg
     3721 tgatcacgca ccggttctcg taccaggagt atgagaaggg tttcgaggcg atgatcgggg
     3781 gcaactgcgg gaaggtgatt ctggactgga ccacgatctg actattgccg cgggtcatca
     3841 acccgtccca ggaacaggat ggccccggtc ttcgtctcgc ggatcataaa gagaaacgga
     3901 tggtcggcgt tgaagacgat gggcggcggc tcggggcgcg ccatgtgcat ggcggtggca
     3961 accatgactg ttgaggtagc tgcggcggct tcggtgccct gctcgttcac atcgatgaac
     4021 gctttgtgaa tcgcggcgga gatggagaaa tcgggccggc ccgtcatgcc ggagaagtcg
     4081 gccgccggcg tgaaagcctg gggcatgccc atcgccgaga gcgtgccgct cagctcgaac
     4141 tgctgcgtca tcgtgaagcg cgggaaggtg aggatgacct tgtcga
//
LOCUS       DASZYN010000142         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_325837, whole genome shotgun sequence.
ACCESSION   DASZYN010000142 DASZYN010000000
VERSION     DASZYN010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325837"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2181)
                     /locus_tag="VHE33_03730"
     CDS             complement(<1..2181)
                     /locus_tag="VHE33_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT96590.1"
                     /translation="MFSVVEGVLLRPLPYPRADRIVRIFLDSPMYPHFPLNPWDFRDY
                     RDRSHSFTGLAAFTRSDLQLSSGSGQPEMLTGFGITAGFFDVLGLRPELGHEFDRGNE
                     VPNSARQAILSDRLWRTRFAGDPNIVGRKITLNAMPFTVVGVMPPGTDHPGNEYHPVA
                     YGQHVDVWTPFWFDGNPSQRGSHYIEGFARLKDGVTAGQAQSEMNAIMTELVREHGNQ
                     VNPWRVLVVPLEQEIVGASRPLLLMLLGAVGMVLLIACANAANLLLARAASRRRELAV
                     RLALGAQRSRLVRQLLTESLVIALAGGALGLALAMGGVRAIVSLLPSDFPRAGDIRVN
                     APVFWFTAAVAMGTGILFGLIPALQASRSDPRQGLHDGGRSLSSSRHQQRLRNSLVVS
                     EVTLACVLLIGAGLMLRSLLAQLHLNAGFSRDHVLTATLSLPEADYKDGRPVAQFYQE
                     LLSGLKTVPGVQTAGAGSDLPWTGWDENTSFDIEGKQPPPNTFFHARYHTATPDYFRA
                     LGTPLVAGRFFTDADKHGAPEVLIINAAMAKKYWPGEDATGKRITFEDHPTEKDWVRV
                     VGVVGDVKDKPNSVGVEPGFWWPHAQAPYHDMAVAVRFDGDPGAMVTVLRDEVHRLNP
                     GLAVAHVQLMDQIVEESVAAPRMQFILVGLFGALAIVLAGIGTYGVIAYAVSQRTTEF
                     GLRMALGAQRGDLMRMVLGQGARLVIPGAAAGVLLALALGRAMKS"
BASE COUNT          422 a          810 c          562 g          396 t
ORIGIN      
        1 gctcttcatc gctcggccca gcgccagcgc cagcaacact ccggctgccg ctccgggaat
       61 taccagccgc gcgccctggc caagcaccat ccgcatcagg tcgccgcgtt gcgcccccag
      121 cgccatccgc aatccgaact ccgtcgtccg ctggctcact gcatacgcaa tcacgccgta
      181 ggtcccgatc cccgccagca caatcgccag cgctccgaag aggccgacca gaatgaattg
      241 catccgcggc gccgccaccg attcctccac gatctgatcc atcaactgca catgcgctac
      301 cgccagtcca ggattcagcc gatgcacttc atctcgcaat accgtcacca tcgcacccgg
      361 atcgccgtcg aaccgcaccg ccaccgccat atcgtgataa ggagcctgcg cgtgcggcca
      421 ccagaatcca ggttccaccc ccacgctgtt cggcttatcc ttcacatcgc cgacaacccc
      481 caccaccctc acccagtcct tttccgtcgg atgatcctcg aacgtgatcc gctttcctgt
      541 cgcatcctca cccggccagt acttcttcgc catcgccgca ttgatgatca ggacctcggg
      601 tgcgccatgc ttgtccgcat cggtgaagaa ccgccccgcc accagcggcg ttcccaatgc
      661 gcggaaatag tcaggcgtgg ccgtgtgata tcgggcatgg aagaaggtgt tgggcggtgg
      721 ctgcttcccc tcgatatcaa agctcgtgtt ctcatcccag ccggtccagg gcagatcgct
      781 cccggcgcct gctgtctgca cgcccggcac cgtcttcaac ccgctcaaca attcctgata
      841 gaactgcgcg accggccggc catctttgta atccgcttcg ggaagcgaca gcgtcgccgt
      901 cagcacgtga tcgcggctga atcccgcatt cagatgcaac tgcgccagca gactgcgcag
      961 catcaacccc gcaccaatca gcagcacgca ggccagcgtc acttcactca ccaccagcga
     1021 gttgcgcagg cgctgctggt gtctgctgct gctcaggctc ctgcctccat catgcaatcc
     1081 ctgccgtgga tcgctgcgtg aggcctggag agccgggatg agtccgaaca gaattcccgt
     1141 ccccatcgcc accgcggccg taaaccagaa caccggcgcg ttcacacgaa tatctccagc
     1201 gcgcggaaaa tcgctcggca gcagagacac gatcgcacgc acacctccca tcgccagggc
     1261 cagccccagc gctccgcctg ccagcgcaat cacaaggctc tccgtcagta attgccgcac
     1321 cagacggctt ctctgcgccc ccagtgccag ccgcaccgcc agttctctcc ttcggcttgc
     1381 cgcgcgcgcc aacagcagat tcgctgcatt cgcacatgcg atgagcagca ccatgcccac
     1441 ggcccccagc agcatgagca acagcggccg gctcgccccg acgatctcct gctccagcgg
     1501 caccaccagt acacgccagg gattcacctg gtttccgtgt tcgcgcacca gttccgtcat
     1561 gatcgcattc atctcagact gtgcctgtcc cgccgtcact ccatctttca gacgcgcgaa
     1621 tccctcgatg taatgcgatc cgcgctgcga tggatttccg tcaaaccaga acggcgtcca
     1681 cacgtcgacg tgctgcccat aggccacggg atgatattcg tttcctggat ggtcggttcc
     1741 cggcggcatc acacccacga ctgtgaacgg catcgcgttc agcgtgatct tgcgcccgac
     1801 gatgttcgga tcgccggcaa accgcgtccg ccaaagccgg tcgctcagga tcgcctgtcg
     1861 cgcgctgttt ggcacttcgt tgccacgatc gaattcgtgc cccagttccg gccgcaggcc
     1921 cagcacatca aagaagccgg ccgtgattcc gaatccggtg agcatctccg gctggcctga
     1981 gccgctcgac agttgcaggt ccgatcgcgt aaacgcagcc agccctgtga atgaatgact
     2041 ccggtcccga tagtctcgaa agtcccacgg gttgagcgga aagtgtggat acatcgggct
     2101 atcgaggaaa atcctcacga tccgatccgc tctcggataa ggcaatggcc gcagcagcac
     2161 accctccaca acgctgaaga tcgcgctgtt
//
LOCUS       DASZYN010000143        11049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_774185, whole genome shotgun sequence.
ACCESSION   DASZYN010000143 DASZYN010000000
VERSION     DASZYN010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 11049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11049
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774185"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2340
                     /locus_tag="VHE33_03735"
     CDS             <1..2340
                     /locus_tag="VHE33_03735"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HVT96591.1"
                     /translation="SSPKMTVSPTSVSFGAVGLNTLASKSVTITSSGNAALTLNSVTA
                     SGTGFAVSGGSFPITLSPGQTATLTISFDPATAGNFTGSVAISSNAATATISLSGTGQ
                     APATLSALSCSTGSLTGAVTDACTVSLTSAATTATAVALTSSSSAVTVPASVTIASGA
                     TSAVFTATAAAVSTNQTATLTATAAGVSKTFSVVLNASTPGLTLSSTSVAFGTDALNT
                     TVTKTVTLTSSGTAALTINAASVTGTGFGDSGMTFPVTLNPGQTATLTISFDPTTAGN
                     FTGSVAISSNAATATISLSGTGQAPATLSALSCSNGLLTGAGTDACTVSLTSAATTAT
                     AVALTSSNSAVTVPASVTIASGATSAGFTATATAVSSNQTATLTATAAGVSRTFNLNL
                     NASAPALTLSSTSVAFGTDALNTTVTKTVTLTSSGTAALTINAATVSGTGFGDSGITF
                     PVTLNPGQTATLTISFDPTTAGNFTGSVAISSNAATATISLSGTGQAPATLSALSCSK
                     GSLTGAGTDACTVSLTSAATSATAVTLTSSSSAVTVPVSVTIASGATSAGFTATATAV
                     STNQTATLTATAAGVSKTFAITLNATEPSLTLSSSSVAFGSVQLNTPATQSVTMTSSG
                     TAAVTVSAGSVGGAGFSMPGAKFPMTLNPGQTATLDLQFNPSTAGPATGTLTLTSNCS
                     MGAMNVALSGTGMATASYTVDLSWNAPASSSDPVVGYRIYRATNGGAYARLNSSINQP
                     ATFSDTTVKSGSTYNYEVVSVDAAGVESTPSNVYTAAIP"
     gene            complement(2564..2944)
                     /locus_tag="VHE33_03740"
     CDS             complement(2564..2944)
                     /locus_tag="VHE33_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HVT96592.1"
                     /translation="MTGTRLDKWLWAARFFKTRTLAAKSCELGRVEANHQPARSSREV
                     RIGDLLSIRTEGGDFEVEVLGLSDVRGPAAAAQQLYRETESSREARRKLAEERKAGLY
                     FTPAPVGRPSKRDRRKIHRFRNDL"
     gene            3415..3855
                     /locus_tag="VHE33_03745"
     CDS             3415..3855
                     /locus_tag="VHE33_03745"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96593.1"
                     /translation="MFRGSHITRVDEKGRLKLPADFKRQIDEEYGGQFYITSRDGKTA
                     EIHPMREWEAFEKKLAAVPTSSLAKQKLLKWINYFGQQAEMDGQGRVLLPQTLREKAK
                     LMAEVVVYGNEAHLMVSNHDDYEQDLASNPMTMADSAELEKYGV"
     gene            3882..4850
                     /gene="rsmH"
                     /locus_tag="VHE33_03750"
     CDS             3882..4850
                     /gene="rsmH"
                     /locus_tag="VHE33_03750"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HVT96594.1"
                     /translation="MTERAESNPRHVPVLLQLAIRMLNVHRGGVVVDATLGNAGHASE
                     IARHLGPEGKLIGFDRDPDAMALATHRLEELKRELGAAMPQVVLHDVEFSRMAERLAG
                     EKVDGLLADFGVSSMQFDQAHRGFSFQADANLDMRMNPREGATAEQVVNQAGEKELAD
                     LIYEFGEERRSRRIARAIVRSRPVTTTAQLAAIVAGAAGSMKGERIHPATRTFQALRI
                     YVNSELSEIDALLAVAPEVLKPGGRLVTISFHSLEDRRVKDALREGARQGIWEVLTRK
                     PLTADAEETDRNPRARSAKLRAAERIEQGTGSKASGEGEERWRHRR"
     gene            4832..5344
                     /locus_tag="VHE33_03755"
     CDS             4832..5344
                     /locus_tag="VHE33_03755"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HVT96595.1"
                     /translation="MATQAIAAPVIGGLGRDMRWVDEHNAVLLAAQRRGRRGPTPEAL
                     FTKRIDNSRLVKANDPARGREMRSFAYAMAFLLSLVMIYGWQHFSAIECGYKLETARQ
                     QMQGLEEQNRELRLTEAQLADPSRIDTMAKNLGLTAPQPGQVVHPTANPDLVGGAVMA
                     ASHPAVPAMH"
     gene            5373..7754
                     /locus_tag="VHE33_03760"
     CDS             5373..7754
                     /locus_tag="VHE33_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HVT96596.1"
                     /translation="MNLPAFSSDPSPDWGDPAPPTGAHHAGKRRGGISSVFKSKPFLV
                     TPIRQTRILGMLGFFFFWAVLIVGRLFWLQLIEHSEWAERAERQQERTFTVAPRRGIL
                     YDRNLHELAMTVVAQSIYADPAEIGDQKATYAATLAKIVHTNPDDRFTSEKQILARLT
                     DSRNFAWVARKQSPAVIAKVQALKLKGIYFQKEFKRFYPDQQLAAQMLGYVGLDDNGL
                     AGVELQRDKELHGTPGRMLTAIDARRHVLDSQERDPQPGENLVLTVDSNIQFMAEQAL
                     DRNMERTDAANGTIVVQDPHTGQILALAIRPTFDPNNFRHTDPSLLRDHAVSDVYEPG
                     STFKLVTYAAALEEKVVTPDSMIPTLGGVINVAGRLVHDDRDAVIFESHHQNVLTANQ
                     ALWESSDVAAVELAQRMGPERFYQYIQGFGFGKRSGIELPGETRGLVKPPSRWQPTTI
                     GSIPMGQEIAVTPIQLVTMASAIANGGVYLPPRIVLESTKELRGNPDLRPAAFRPEGE
                     LPDPLPPGAHRVISEMTSAQMRKMMEGVVLYGTGSTAQLNGYSAAGKTGTAQKIDPAT
                     RTYSKTKYVASFVGFAPVSDPAVTIAVIMDSPMKGGHFGHEASAPVFQDLAQKVLEYL
                     GVAHDQDLKPVKELAKKSKPVKEQDPQEHAEDLQSLFAEVNNLPTDDPLRNPANQGPV
                     PTPVAAAQPSESAGPSVSTSEANARPPVQLVSAGTNGKPQEASKAGHDVTVAVQRVAV
                     PSFAGKSLREVVQQASGVGLGVQIIGSGMARDQVPAAGTMVPAGTEIVVRFAQ"
     gene            7823..9343
                     /locus_tag="VHE33_03765"
     CDS             7823..9343
                     /locus_tag="VHE33_03765"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HVT96597.1"
                     /translation="MRFEEILSGAEVVRRAGANVADGIAIRGVEYDSRRVGPGSLFLA
                     MQGETTDGNRYIGKAIEQGAVGIVTDSASAFATVAREHPEIAIAEVPAGAGRRAMAAI
                     AANFFGHPERQLALSGVTGTNGKTTTAFLLEAMLNSVGRKTVLVGTIENHVAGVVRST
                     PHTTPESRDLLALFREGADAGASEAVMEVSSHALAQGRVFGLPYDVAIFTNLTQDHLD
                     YHGTMENYFAAKQMLFNGALGAPPRVAVINIEDAHGAKLAEIARQAGVQVFSYGLHRG
                     DLRAEGPAMGAAGMRFRLLTPAGTVDVRTRLTGKVNLYNLTAASGAALARGLSPEQIA
                     NGIPTLAHVPGRFQTIDEGQKFAVVVDYAHTDDALRNLTSLAREFVGSSGRVITLFGC
                     GGDRDRAKRPKMGRAAGEGSDFVVLTSDNPRSEDPEAIIADVLPGLIETATKFVVEPD
                     RARAIRLAIEAAHPGDIVLLAGKGHEKEQILRHGTIPFDDADVAAAAIREAVGARA"
     gene            9340..10767
                     /gene="murF"
                     /locus_tag="VHE33_03770"
     CDS             9340..10767
                     /gene="murF"
                     /locus_tag="VHE33_03770"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HVT96598.1"
                     /translation="MKLTLEQIARWSAAEIRASGAAAPELIPATGYSIDSRTLQPGDL
                     FFAIHGERFDGHDFVTAALERGACAAVVARDRVSALSAAQGGAHALLATDDVLASLQR
                     LAAAVRRHWGKRVVGITGSAGKTTTKEAIASVLGTRFNVLKSHGNLNNGYGLPLQLLR
                     LEPEHDVAVIEMGMSAAGEIAALCHIAAPDWGVVTNVGHAHTEFFADGIAGVARAKYE
                     LVESLPRHGVAFLNCDDPYVSQFGRDFEGKAVYFGRGPCGDPHAERIESVGADGLKIE
                     VRAGEQHCELHLHLPGEHNGANALAAIAVGVEAGIPLAQCCAALAQLRPEEKRGQLLT
                     IRGATIINDVYNSNPEALKSMIRTLAGMPAQRRILVAGEMLELGREAEALHAACGKAA
                     ALAGIGIVIGVRGNARSIVDAALQNGAEALLMETPEAAGEWLRENLKPGDAVLLKGSR
                     GVRLERALEGLQGSEKGTGPQGSAG"
     gene            10806..>11049
                     /locus_tag="VHE33_03775"
     CDS             10806..>11049
                     /locus_tag="VHE33_03775"
                     /inference="COORDINATES: protein motif:HMM:NF022030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96599.1"
                     /translation="MLYWLLYKKLYLYFSPFRIFRYLTFRTVFASLTALLIMLIIGPY
                     VIERLREFQIGQYIREEGPKAHQKKAGTPTMGGVLIC"
BASE COUNT         2218 a         3123 c         3724 g         1984 t
ORIGIN      
        1 tcctcgccaa agatgaccgt gagcccgacg agcgtgtcgt ttggcgccgt cggcctgaat
       61 acgctggcct cgaagtcagt gaccatcaca tcttccggga atgccgcgct gaccctgaac
      121 agcgtcacgg cgagcggcac aggcttcgcg gtctccggcg gaagcttccc gataacgctg
      181 agcccaggtc aaacggcgac gctgacgatc agcttcgatc cggcgacggc agggaacttc
      241 accggctcgg tggcgatcag cagcaacgcg gcgacggcga cgatcagtct gagcggaacc
      301 ggacaggctc cagcgacgct gagcgccctt tcgtgcagca cgggatcgct gactggagcg
      361 gtcacggacg cctgcacggt atcgctgact tcggcagcga ccactgcgac ggcggtggcc
      421 ctgaccagca gcagcagcgc ggttacggtg cccgcctcgg taacgatagc gtccggcgca
      481 accagcgctg tcttcacggc gacggcagcc gcggtgagca cgaatcaaac cgccaccctg
      541 accgcgacgg cagccggcgt gtcgaagacg tttagcgtgg tcctgaacgc gagtacgcct
      601 gggctgaccc taagcagcac cagcgttgcg ttcggtacag atgcgctgaa cacgactgtg
      661 acgaaaactg tgacgctgac gtcttcaggt acggcggcgc tgaccatcaa tgcggcttcg
      721 gtgaccggca ccggctttgg tgattccgga atgacgttcc cggtcacgct gaatccaggt
      781 cagacggcga cgctgacgat cagcttcgat ccgacgacgg caggaaactt taccggctcg
      841 gtggcgatca gcagcaacgc ggcgacggcg acgatcagcc tgagcggaac cggacaggct
      901 cccgcaacgc tgagcgcgct ttcctgcagc aacggattgc tgacgggtgc ggggacagac
      961 gcctgcacgg tgtcactgac atccgcggcg accactgcga cggcggtggc cctgaccagc
     1021 agcaacagtg cggtcacggt gcccgcctcg gtaacgatag cgtccggcgc aacgagcgcc
     1081 ggcttcacgg cgacggcaac ggcggtgagc tcgaaccaaa ccgctacgct gacggctaca
     1141 gcggcaggtg tgtcgagaac cttcaattta aatctgaacg cgagtgcgcc tgctctgacg
     1201 ctgagcagca ccagcgttgc attcggtaca gatgcgctga acacgactgt gacgaaaacg
     1261 gtgacgctca cgtcttcggg tacggcagcg ctgaccatca atgcggccac agtcagcggc
     1321 acgggattcg gcgactcggg aattacgttc ccggttacgc tgaatccagg tcagacggcg
     1381 acgctgacga tcagcttcga tccgacgacg gcggggaact tcaccggctc cgtagcgatc
     1441 agcagcaacg cggcgacggc aacgatcagc ctgagcggaa ccggacaggc tccggcaacg
     1501 ctgagtgctc tctcctgcag caaaggatcg ctgaccggag cgggcacgga cgcctgcacg
     1561 gtatcgctga cttcggcagc gacgagtgcg accgcggtga ccttgaccag cagcagcagc
     1621 gcggttacgg taccggtctc ggtaacgatt gcgtccggcg cgaccagcgc cggcttcacg
     1681 gcgacggcaa cggcggtgag cacgaatcag accgccaccc tgaccgcgac ggcggctggc
     1741 gtgtcgaaga cgttcgccat caccctcaac gctaccgagc cgagcctgac cctcagttcg
     1801 agcagcgtgg ccttcggcag cgtccagctc aacactccag caacccagtc ggtgacgatg
     1861 acgtcgtcgg gtacggctgc ggtgacggtg agtgcgggca gtgtgggcgg cgcgggattc
     1921 tccatgccgg gagcgaagtt cccgatgacc ctgaatccgg ggcagacggc gacactggat
     1981 cttcagttca atcccagtac cgccggacca gcaacgggta cgctcacgct gaccagcaac
     2041 tgctccatgg gagcgatgaa cgtcgcgctg agcgggaccg ggatggcgac tgccagctac
     2101 acggtggatt tgagctggaa tgcgccagcc agttcgtcag acccggttgt gggctaccgc
     2161 atctatcggg cgacgaacgg cggtgcgtat gcccggctga attcgtccat caatcagcct
     2221 gcgactttct ccgatacgac ggtgaagagc gggtcgactt acaactatga ggtggtgagc
     2281 gtcgatgcgg cgggggtcga gagtaccccg tcgaatgtct atactgcagc gattccgtag
     2341 aagagcacgc agataggttc tgcggaatcg gtttgattcg gcgtcagccg cgatctggca
     2401 ggacggctga cccgggcgga gcatgaattt tcgtgctccg cccgtttctt tttcgagact
     2461 ttccagcgac acgatgacgg gcgcggtcga tacggagggt tccagtactc ctggcaacga
     2521 tgcgcgccct gctaacgtct tcgtgcggcc caaccaccag aagctagaga tcgttgcgga
     2581 accggtggat ctttctccgg tcgcgcttgg aaggacgtcc caccggggcc ggtgtaaagt
     2641 acaggccggc tttgcgctcc tcggcgagtt tacggcgcgc ttcccggctg gattctgttt
     2701 cgcgatagag ctgctgggcg gcggcagcgg gtccacgaac gtcgctcagc ccgaggacct
     2761 cgacttcgaa gtcgcctcct tcggtccgga tcgagagcag gtcgccgata cgaacctcac
     2821 gggaggaacg cgcgggctgg tgattcgctt ccacacgccc cagctcgcag gatttcgcag
     2881 cgagcgtgcg ggttttgaag aagcgagccg cccagagcca tttgtcaaga cgcgtgcccg
     2941 tcatagaggc agcgtacctg cttgcagcgg attgagaaac gatccttacg accttcagtc
     3001 ggagggcaaa atggcagaaa accggtccag gcgaagattt ggcgaacatt accgcgaacc
     3061 ggggctcctg acaaggggcc gagactcaat tttcttgccc cggaatcact ctggataacc
     3121 gcaacctgta gtggttcatc gcggtaaaac acccaagtcg tgcggttttg gcttgtaaat
     3181 cgcgggaagt gtgtggttta gggatcgcca atattcatcg gcagagcgat tttccacttc
     3241 accgggagaa tcacatcggt tacgctgcct caagtgggaa gttctggagt gaagtggtca
     3301 aaattcccaa ctccggaaag cccccaaaaa tggcgtccca gcgcccacag tagcaccgtt
     3361 tgagggcgat aacgggccga ttttgtgggg gttccgcctc aggcacggga ttcgatgttt
     3421 cgtggcagcc acattacgcg ggtcgacgaa aaagggcggc tcaagctgcc ggcggacttc
     3481 aagcgccaga tcgatgagga gtacggaggg cagttctata tcaccagccg ggacggcaaa
     3541 acggctgaga ttcacccgat gcgggagtgg gaagcgttcg agaaaaagct ggcggcggtg
     3601 ccgacttcca gtctggcaaa gcagaagttg ctgaagtgga tcaactactt cggccagcag
     3661 gcggagatgg atgggcaggg cagggtgtta ttgccgcaga ctctgcgaga gaaagccaag
     3721 ctgatggcag aagttgtggt gtacggcaat gaagcgcacc tgatggtttc gaatcacgac
     3781 gattatgagc aggatctggc gagcaatccg atgacgatgg cggactcggc cgagctggag
     3841 aagtacggag tttagctgga tacacccctc agggggagca catgacggaa cgggcagaaa
     3901 gtaaccccag gcatgtgccg gttcttttac aacttgcgat ccgaatgttg aatgtgcacc
     3961 ggggcggcgt ggtggtcgac gcgacgctcg gcaatgcagg gcatgcgagc gaaatcgcca
     4021 gacacctggg gccggaaggg aagctgatcg gcttcgaccg ggatccggac gcgatggcgc
     4081 tggccacgca tcggctcgaa gagctcaaac gggagctcgg agccgcgatg ccgcaggtgg
     4141 tcctgcacga tgtggagttc tcgcggatgg ccgaaaggct ggccggagag aaggtcgatg
     4201 gtctgctggc agatttcggg gtcagctcga tgcagttcga tcaggcgcac agaggattta
     4261 gttttcaggc ggacgccaac cttgatatgc ggatgaatcc gcgtgaagga gcaaccgccg
     4321 aacaagtggt aaatcaggcc ggagaaaaag agctcgccga tctgatttac gaattcggag
     4381 aggaaaggag gtcgcggaga atcgccagag ccattgtccg gtcgcggccg gtgacgacga
     4441 cggcacaatt agccgcaatc gttgcaggcg cggccgggtc aatgaaaggt gagcgaattc
     4501 atccggcgac caggaccttt caggctcttc gaatttatgt gaacagcgag ctgagtgaga
     4561 tcgacgcgct gcttgctgtt gcgccggagg tgctgaagcc gggaggaagg ctggtcacga
     4621 tcagcttcca ctccctggaa gaccggcgcg tcaaagatgc gctgcgcgag ggagcgcggc
     4681 agggaatttg ggaagtgctg acgcggaagc cgctgacggc cgacgcagag gagacggatc
     4741 gcaacccgcg ggcgcgcagc gcaaagctgc gggcagcgga aagaatcgaa caagggacgg
     4801 gcagtaaagc gagcggcgag ggagaagagc gatggcgaca caggcgatag cggcaccggt
     4861 aatcggtggt ttgggccgcg acatgcggtg ggtagacgag cacaatgctg ttttgctggc
     4921 ggcgcagcgc cgcggccggc gcggacccac gcctgaggcg ttgttcacca agcgcatcga
     4981 caattcgcgg ctggtgaagg cgaacgatcc ggcgcgcggc cgggagatgc gcagcttcgc
     5041 ctatgccatg gctttcctgc tttcgctggt gatgatttat ggatggcagc acttcagcgc
     5101 catcgagtgc ggctacaagc tggaaacggc ccgtcagcag atgcaggggc tggaggagca
     5161 gaatcgggag ttgcgcctga ccgaggccca gctggccgat ccttcccgca ttgatacgat
     5221 ggcgaagaac ctcggactta cggcgccgca accgggacaa gtggtacacc cgactgcgaa
     5281 tccggacctg gtcggcggag ccgtgatggc ggcgtcgcat cccgccgttc ccgccatgca
     5341 ctgagctttc ccccgagagg agaggtctcg ttgtgaatct gcctgcgttc tcctctgatc
     5401 cgtcacccga ctggggtgac cctgcaccgc ccacaggagc ccaccacgcc ggaaaacgac
     5461 gtggtgggat ttcttcggta tttaaaagca aacctttcct ggtcacgccg attcggcaga
     5521 cccgcatcct gggcatgctc gggttcttct ttttctgggc ggttctgatc gttggccgtc
     5581 tgttttggtt gcagctcatt gagcacagcg aatgggcgga gcgcgcggag cggcagcagg
     5641 aacgaacctt cacggtagct ccgcgacggg gcatcctgta cgaccgcaat ctccacgaac
     5701 tggcgatgac agtggtagca cagtcgatct atgcggatcc cgcggagatc ggcgaccaga
     5761 aagccaccta tgccgccact ttggcgaaga tcgtccatac caatcccgac gatcgtttca
     5821 cgtcggagaa gcagatcctg gcgcgattga cggattcgcg caatttcgcg tgggtcgcgc
     5881 gaaagcagtc tcccgcagtc atcgcaaagg tgcaggcgct caagctgaaa gggatttatt
     5941 ttcagaagga attcaagcgc ttctatccgg atcagcagct ggcggcgcaa atgctggggt
     6001 acgtcggcct cgacgacaac ggtctggccg gcgtggaact gcagcgggac aaggaactgc
     6061 atggaacgcc gggccgcatg ctgacggcga ttgacgctcg acgccacgtc ctggacagcc
     6121 aggagcgcga cccgcagccg ggtgagaatc tggtcctgac tgttgactca aatatccagt
     6181 tcatggcgga gcaggcgctc gatcgcaaca tggagcgcac cgatgccgcc aacggaacga
     6241 tcgtggttca ggatccgcac accgggcaga ttctggcgct ggcgatccgg ccgaccttcg
     6301 atccgaacaa ctttcgccac accgatcctt ctctgctgag ggatcacgcc gtgagcgacg
     6361 tttacgaacc gggctcgacg ttcaagctgg tcacgtatgc tgcggctctg gaagagaagg
     6421 ttgtcacgcc ggactcgatg attcccactc tcggcggcgt gatcaatgtt gccggccgtc
     6481 ttgtccacga tgaccgcgat gcggtgatct tcgagagtca tcaccagaat gtgctcaccg
     6541 ccaatcaggc gttgtgggaa tccagcgacg ttgcagcggt cgaactcgct cagcgtatgg
     6601 gccccgagcg cttctatcag tacattcagg gtttcggatt cggcaaacgt tccggcatcg
     6661 agctgcccgg cgagacgcgt ggtttggtga agccgccatc gcgctggcag ccgacgacga
     6721 ttggctcgat ccccatggga caggaaattg ccgtcacgcc gatccagctg gtaacgatgg
     6781 cttccgctat tgcgaacgga ggcgtgtatc tgccgccccg catcgtgctc gagagcacga
     6841 aagagctgcg gggaaacccg gatctgcgac ccgccgcgtt ccgcccggaa ggcgagctgc
     6901 ccgatccttt gccgccggga gcgcatcgtg tgatctccga gatgacgtcg gcgcagatgc
     6961 gcaaaatgat ggagggcgtg gtcctgtacg ggaccggcag caccgcgcag ctgaacggct
     7021 acagcgctgc cggcaagacc ggcaccgcgc agaagatcga tccggcgacc cgtacctact
     7081 cgaagacgaa atacgtggct tcgttcgtcg ggttcgcgcc ggtcagcgat ccggcggtga
     7141 cgattgcggt cattatggat tcgcccatga agggtggcca tttcggccat gaggccagcg
     7201 caccggtttt ccaggatctg gcacaaaagg ttcttgagta tctgggagtg gcgcacgacc
     7261 aggatctgaa gccagtgaag gagttggcga agaagagcaa gccagtgaaa gagcaggatc
     7321 cgcaggagca tgccgaagat ttgcagtcac tgtttgccga agtgaataac cttccgaccg
     7381 acgatccgct gcggaatccg gcgaatcagg gaccagtgcc gaccccggtc gcggcggcgc
     7441 agccctcgga gtccgccgga ccatccgttt ccaccagcga agccaatgcg cgcccaccgg
     7501 ttcagcttgt ttctgccggg accaatggga agccgcagga ggcctcaaag gcagggcacg
     7561 atgttacggt ggcggtgcag cgcgttgcgg tgccgtcttt tgcagggaag tcgctgcgtg
     7621 aagtggtcca gcaggccagc ggtgtgggac tgggcgtgca gatcatcggc tcgggaatgg
     7681 cgcgcgatca ggtgccggcg gctggaacga tggtacccgc tggaacggag attgtggttc
     7741 gttttgcgca gtgattcctg cgatccggca ggattcgcgg tgatagttcg agcattcgat
     7801 cgacgcttac aatcagcttg atatgcgctt tgaggaaatc ctgagcggcg ccgaagtcgt
     7861 gcgcagagcc ggtgcgaacg tagcggacgg aatcgcgatt cggggtgttg agtacgactc
     7921 gcgacgggtc ggccccggct cgctcttcct tgctatgcag ggggagacga cggacgggaa
     7981 tcgctacatt ggcaaggcta tcgaacaggg cgcggtgggg attgtcacgg attcggcttc
     8041 agcttttgcg acggttgcca gggagcatcc ggagattgcc atcgcagaag tgccggcggg
     8101 agcgggacgc cgtgcgatgg cggccatagc cgcgaatttc ttcggtcatc ccgagcgcca
     8161 gctcgcgttg agcggggtga cgggtaccaa cggcaagacc acgacggctt ttcttctgga
     8221 agcgatgctg aactcggttg gacggaagac ggtgctggtg ggaaccatcg aaaaccacgt
     8281 ggccggcgtg gtgcggtcga cgccgcatac caccccggag tcacgcgatc tgctggcatt
     8341 gttccgcgaa ggagcggatg caggagcgag cgaggcggta atggaggttt cgtcgcacgc
     8401 gcttgcgcag ggccgcgtgt ttgggctgcc ctatgatgta gcgatcttca ccaatctgac
     8461 ccaggatcac ctcgactatc acggaacgat ggagaactat tttgcggcca agcagatgct
     8521 cttcaacggg gcgttggggg cgccgccgcg ggttgcggtc atcaatatag aggacgcaca
     8581 cggagcgaag ctggccgaga tcgcacgcca ggccggggtt caggtctttt cctacggtct
     8641 gcatcgaggg gatttgcgag ctgaaggccc agcgatgggc gcagcaggga tgcggtttcg
     8701 gctgttaaca cccgccggaa cggttgacgt tcggacccgg ctgaccggca aagtgaatct
     8761 ctataactta acggcggcgt cgggggctgc gttggcgcgg ggccttagcc cggagcagat
     8821 cgccaacggt attccaactc tggcgcatgt gcccgggcgg ttccagacca tcgatgaagg
     8881 gcagaaattc gccgtcgtgg tggactacgc acacaccgac gatgctctgc ggaacctgac
     8941 atcgctggcg cgggaattcg tgggtagctc cggccgggtg atcacgctgt tcggatgtgg
     9001 aggagatcgc gaccgggcca agcgcccgaa gatgggccgc gcagcgggag agggaagcga
     9061 tttcgtagtg ttgacttcgg ataatccgcg cagcgaggat ccggaggcga tcatcgcgga
     9121 tgtcctgccg gggttgatcg agacggcgac gaagtttgtc gtggaaccgg atcgcgcccg
     9181 ggcgattcgg ctggctatcg aagccgcgca cccgggagat attgtcctgc ttgccggaaa
     9241 gggacacgag aaggagcaga ttctgcggca cggtacgatt ccattcgacg acgccgacgt
     9301 cgcggcggcg gcgatccggg aagcggttgg agcgcgcgca tgaagcttac gctcgagcag
     9361 attgcccgct ggtcggcggc ggagattcgt gccagcgggg cggctgcgcc ggaattgatc
     9421 ccggcgacgg gctactcgat cgactcgcga actctgcagc cgggagatct gttcttcgcg
     9481 attcatggcg aaagattcga cgggcacgat ttcgtgacgg cagcgctgga gcggggcgcg
     9541 tgcgcggcgg ttgtggcgcg cgaccgggtc agcgcgctgt ccgcggcgca gggcggtgcc
     9601 catgcattgt tggcaaccga cgatgtgctc gcctctttgc agagactggc ggccgcggtc
     9661 cggcgtcact ggggcaagcg ggtggtgggc atcaccggat cggcaggcaa aacaacgacc
     9721 aaagaagcca tcgcttcagt cctgggcact cggtttaacg tgctcaaatc acatggcaat
     9781 ctcaataatg gatacgggct gccgctgcaa ttgctgcgtt tggaaccgga gcacgatgtg
     9841 gcggtgatcg agatggggat gtcggccgcc ggcgagatcg cggcgctgtg ccatatcgcc
     9901 gctccggact ggggagtagt gacgaatgtc ggccacgcgc atacggagtt ttttgccgac
     9961 ggcattgcgg gagtggcgcg ggcgaagtat gagctggtgg agtccctgcc gcggcatgga
    10021 gtggcctttt tgaattgtga cgatccgtat gtctcgcagt tcggtcgcga cttcgagggg
    10081 aaagcggtct acttcggacg cggaccctgt ggcgatccgc atgccgagag gatcgagtct
    10141 gtgggagcgg acggcctgaa gatcgaggtt cgcgcgggcg agcagcattg cgaactacat
    10201 ctgcacctgc cgggcgagca caacggggcg aatgcactgg ccgcaatcgc tgtcggtgtt
    10261 gaggccggca ttccgcttgc ccagtgctgt gccgcactcg cgcaactgcg ccccgaggaa
    10321 aagcgcgggc agttgctcac gattcgaggc gcgaccatta tcaacgacgt ctacaattcc
    10381 aaccctgagg ctctgaagtc gatgatccgg acactggctg gaatgccggc gcagcggcgg
    10441 attctggttg cgggcgagat gctggaactg ggccgggagg ctgaggccct tcatgcagca
    10501 tgcggaaaag cggcggcact ggccggcatc ggaattgtga ttggcgttcg cgggaatgcg
    10561 cgatccatcg tcgacgccgc cctgcagaac ggagccgaag ctttgttgat ggagacgccg
    10621 gaggccgctg gcgaatggct aagagaaaat ctgaagcctg gcgacgcggt tctgctgaaa
    10681 ggctcgcgtg gagtgaggct ggagcgtgcg ctcgagggtt tgcagggatc agaaaaaggc
    10741 acgggaccgc agggttcggc aggataagat aagcgggaaa atctaccagt ttttgtggag
    10801 gaagtttgct ttactggctg ctttataaga agctctacct ctacttcagt ccattccgga
    10861 tctttcgata cctcacgttc cgcacggtgt tcgcctcgct gacggcgctg ctgatcatgt
    10921 tgatcattgg tccctacgtg atcgaaaggc tgcgcgagtt tcagatcggc cagtacatcc
    10981 gcgaggaggg ccccaaggcc caccagaaga aagcgggcac gccgaccatg ggcggagtgc
    11041 tcatctgca
//
LOCUS       DASZYN010000144         4836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_517719, whole genome shotgun sequence.
ACCESSION   DASZYN010000144 DASZYN010000000
VERSION     DASZYN010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517719"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1746)
                     /locus_tag="VHE33_03780"
     CDS             complement(<1..1746)
                     /locus_tag="VHE33_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HVT96600.1"
                     /translation="MHLSVSTHRLLRCVSAAVVLVASSAFAQNSTAPKPDNRPWMNKS
                     LSPDERADLVIKQMTLDEKMNFVHGTGWNGLREDAEIPKGSLGGAGYVQGVARLGIPG
                     INMADSAVGVRLAAQGSHYATLLPSVLGAASSWDQKALFLYGSVIGRELRDTGYNMSI
                     GGGVDIARDPRNGRNFEYAGEDPVLAGTMVGELARGVQANQVMGDIKHYAFNDQETGR
                     TTVNVSIDERSARESDLLAFQIAIGLSHPAGVMCSYNRVTVPPNSPTDWACENDYLLN
                     KVLKKDFDFKGFVLSDWGGTHSTAHAAVSGLDQEQPGSKYFGDPLKQAVQSNKVPMSR
                     LDDMVHRVLRSMFANGVIDNPPVTQVVDPFRGRDDAEHIADESIVLLKNSGDMLPLKV
                     ASVQSIAIIGSHADVGVLSGGGSAQVDAPGGNAIDPHPGASRWGEHIYFPSSPLKEIR
                     KHAPDAKVEFDPGTDNAAAAKLAAASQVAIVFVNQPLSEGMDAPTLSLPDNQDALVDA
                     VAAANPHTIVVIESGGPVSMPWIDHVAGVLEAWYPGIGGAQSLANILFGEVNPSGRLP
                     ITFAKTDAQLPHPEVP"
     gene            complement(1995..4118)
                     /locus_tag="VHE33_03785"
     CDS             complement(1995..4118)
                     /locus_tag="VHE33_03785"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HVT96601.1"
                     /translation="MPHGRAFSVASLALACLAFGTAFSSAQSHSDDPADKYIWLEEAN
                     SPRAMDWVKGEDARTAKVFEADPRFSTYNAEALKIIEDPNRLPLPDLRGDEVYNLWND
                     QKNVRGLLRKTTMEDYSTPEPHWKTVLDVDALGKQENTSWVFHGDDCLYPGDEYCIFN
                     LSVGGEDASTSREFNLKQNQFVPSGFVSPHSKQEIAWVDKDTILIARDWGAGTMTQSG
                     YPFVVKEWKRGTPLESAKEVFRGEPGDIFARAYVFHDAQGDQLTVFSRTLSFFESRRW
                     IQTPGGLKPLAIPMKAEPAGLLAGRLLIQIHEDWTPEGGPKFVQGSLLSVRLADAVRD
                     PAHLKPTVVFAPTADEFLSGAATTRSRLILTTLKHVLGRAYVYTPSGEDGWSIRTLDV
                     PDNSTINIESTTDANETFFLSSQGFLTPPSLLQGDAGTGQVKVARSQPPLFDASQDTV
                     EQRFATSKDGTKIPYFIVHRKDMQMNGQNPTLLNAYGGFELSETPRYSATMGKLWLEK
                     GGVYVLANIRGGGEFGPAWHEAGLKTHRQRIYDDFAAVAEDLIARKVTSPRHLGIIGG
                     SNGGLLMGVEMTQHPDLYNAIVIQVPLLDMLRYEHIAAGASWVGEYGSVSVPEEKAFL
                     EKISPYNQLKQDVKYPEPFIFTTTRDDRVGPQHARKFAAKMEEYHEPFLYDEITEGGH
                     GAGADLKETARSWAETYVYLAEKLM"
     gene            complement(4236..>4836)
                     /locus_tag="VHE33_03790"
     CDS             complement(4236..>4836)
                     /locus_tag="VHE33_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96602.1"
                     /translation="LLIGMGLFVALPSLLAAQENGRDNQVEFGVYADYFRFAPGGGTS
                     TNFVGAGGRVGFAYHHMGLEAEMNYDFARNITTTVTNSAGSSTTTTFVKTGVRPLTGL
                     FGPVFHFGPGNFHAFVTGKVGFINFTTTNPDNVTGQQAADAVASVGGSGTHFAAYPGG
                     GVEGFWGPVGLRLEAGDEVYLNNDVYNNLRVTVGPQFRF"
BASE COUNT          976 a         1570 c         1371 g          919 t
ORIGIN      
        1 tgggacctcc gggtgcggca gctgcgcgtc agttttcgca aacgtgatcg gcagacgccc
       61 ggatggattc acttccccga acaggatgtt cgccagcgat tgcgcgccgc cgatccccgg
      121 ataccacgct tcgagaacac ccgcgacgtg atcgatccat ggcatcgaca cagggccgcc
      181 gctttcaatc accacaatcg tgtgcggatt cgcggcggca acagcatcca ccagggcatc
      241 ctgattgtca ggaagggaga gggtcggagc gtccatgccc tcgctcagcg gctgattgac
      301 gaaaacaatg gccacctgcg aggccgcagc cagtttcgca gccgcagcat tgtccgtacc
      361 gggatcgaac tccactttcg cgtcgggtgc atgtttgcgg atctccttca gcggcgacga
      421 aggaaagtaa atatgctctc cccagcgcga tgcacccgga tgcggatcga tggcgtttcc
      481 tccaggcgcg tcgacctgcg ccgatccgcc gccggagaga acaccgacat cggcatgcga
      541 tccgatgatc gcaattgact gcaccgaggc caccttcaag ggcaacatgt cgccgctgtt
      601 cttcagcagc acgatgctct cgtcggcgat gtgctccgcg tcatcgcgcc cgcggaaggg
      661 atcgacgacc tgagtcaccg gcggattatc gatcacgcca ttcgcgaaca tgctgcgcaa
      721 cacgcgatgc accatgtcat cgagccgcga catgggaacc ttgttgctct ggaccgcctg
      781 cttcagcgga tcgccaaagt actttgaccc gggctgctcc tgatcgagcc ctgacaccgc
      841 ggcgtgcgcg gtggagtgcg tcccgcccca gtcggaaagc acgaatcctt tgaagtcaaa
      901 gtctttcttg aggaccttgt tcagcagata gtcgttctcg caggcccagt cggttgggga
      961 gttgggtgga acggtgacgc ggttatacga gcacatcacc ccggcgggat gagaaagccc
     1021 aatggcaatc tgaaaagcca gcagatcgct ctctcgtgcc gagcgctcgt caatggaaac
     1081 gttcaccgtc gtgcgtccgg tctcctggtc gttaaaggcg taatgcttga tgtcgcccat
     1141 tacctggttg gcctgaacgc cgcgcgccag ctcgcctacc atcgtcccgg ccagcaccgg
     1201 atcctcgcct gcgtattcga agttgcgccc gttgcgcgga tcgcgggcga tgtccacgcc
     1261 gccgccaatc gacatgttgt agccggtgtc gcgcagctcg cgtccgatca ccgaaccgta
     1321 caggaacagc gctttctgat cccagctgct ggccgcgccc agaaccgacg gcagcagcgt
     1381 ggcatagtgg ctgccctgcg ccgccaggcg aaccccgaca gcagaatcgg ccatgttaat
     1441 gccgggaatt cccagacgcg ccacaccctg cacgtagccc gcaccgccca gcgatccctt
     1501 cggaatctcc gcgtcttccc gcaatccgtt ccatcccgtg ccatggacga agttcatctt
     1561 ctcgtccaga gtcatctgct tgatgaccaa atcagcccgc tcatccggcg aaagcgattt
     1621 gttcatccac ggtcgattgt cgggtttggg agcagtcgag ttctgagcga aggccgatga
     1681 tgcaacgagc acgaccgcag cggaaacaca acgcagcagg cggtgtgtgg aaacagacag
     1741 atgcatagtc acctcgaacg gagaaatgct aatggcgaga ggaatgcatc gtcaagataa
     1801 aacgtttaac tggatacacg cctgatgaaa cgatcccgga tgccgggacc gaccggcgtc
     1861 aggccgacag ccctgcagtc accgcgacga aaatgcccgg tccaggctca ctacccactg
     1921 caccgccagc gtgttctcgt tgctcggagt cccctgggtg gattctccca ctcgtggccc
     1981 agccaaaggc cgggctacat cagcttctcg gcaagataca catacgtttc cgcccacgaa
     2041 cgcgctgtct ctttcaggtc cgcacccgcg ccatggccgc cctcggtgat ctcgtcgtag
     2101 aggaacggct cgtggtactc ctccatcttt gccgcgaact tgcgcgcatg ctgcggaccc
     2161 acccgatcgt cccgcgtcgt cgtgaagatg aacggctccg gatacttcac atcctgcttg
     2221 agctggttgt aaggcgaaat cttctcgaga aaggccttct cttccggcac gctcaccgat
     2281 ccgtactcgc ccacccagct cgctcccgcc gcgatgtgct catagcgcaa catgtcgagc
     2341 agtggaacct gaataacgat ggcgttgtac aggtccgggt gttgcgtcat ctccacgccc
     2401 atcagcagac cgccattcga cccgccgata atccccagat gccgcggtga agtcaccttg
     2461 cgtgcaatca agtcctcagc cacggcggcg aagtcgtcat agatacgctg gcgatgcgtc
     2521 ttcaggccgg cttcgtgcca cgccggacca aactcaccgc ctccgcgaat gttggccagc
     2581 acgtagaccc cgcctttttc cagccacagc ttccccatcg tcgcgctgta gcgtggcgtc
     2641 tcggagagtt caaaaccgcc atacgcgttc agcagtgtgg gattctgccc gttcatctgc
     2701 atgtccttgc gatgaacgat gaagtacggg atcttcgtgc cgtctttcga ggtggcgaag
     2761 cgctgttcga ctgtgtcctg cgacgcatcg aaaagcggcg gctgcgaacg cgcgaccttc
     2821 acctgccccg tgccggcatc gccctggagc agcgagggcg gcgtcagaaa accctgtgag
     2881 cttagaaaga atgtctcatt cgcatcggtt gtcgactcga tattgatagt gctgttgtcc
     2941 ggcacatcga gcgtccggat cgaccagccg tcttctccgg aaggtgtgta aacgtaggcg
     3001 cgtcccagca catgcttgag cgtcgtgaga atcaaacgcg agcgcgtcgt cgccgcaccg
     3061 ctcaaaaatt cgtccgccgt cggcgcaaaa acaaccgtgg gcttcaggtg cgccggatcg
     3121 cgaacagcat cggccagccg gaccgacagc agcgaaccct gaacgaactt cggcccgccc
     3181 tccggcgtcc agtcctcatg aatctgaatc agcagccgcc cggcaagcag gccggcaggt
     3241 tccgccttca tcggaatggc cagcggcttc aggccgccgg gcgtctggat ccaccgccgc
     3301 gactcaaaga agctgagcgt ccggctgaaa accgtgagct gatcgccctg agcatcatga
     3361 aacacatagg cgcgcgcgaa gatatcgccc ggctcgccgc gaaaaacttc cttcgcgctc
     3421 tccagcggcg tgccccgctt ccactctttc accacaaacg ggtatccgga ctgcgtcatg
     3481 gtgcccgcac cccagtcgcg agcgatcagg atcgtgtcct tatcgaccca cgcgatctcc
     3541 tgcttgctgt gaggtgacac aaagcccgat ggaacgaact ggttctgctt caggttgaac
     3601 tcgcgcgacg tcgaggcatc ttctccgccc actgacaggt tgaagatgca gtactcgtcg
     3661 ccgggataga ggcagtcgtc cccgtgaaac acccagctgg tgttctcctg cttgccaagc
     3721 gcatctacat ccagcaccgt cttccagtgc ggctccggcg tcgaatagtc ctccatcgtg
     3781 gtcttgcgca gcagaccccg aacgttcttc tgatcgttcc agaggttgta aacctcgtcg
     3841 ccgcgcagat ccggaagcgg aagccgattc ggatcctcga tgatcttcag agcctctgcg
     3901 ttatacgtgc tgaaccgtgg atcggcctca aagacctttg ccgtgcgcgc atcctcgcct
     3961 tttacccaat ccatcgctcg cgggctgttc gcttcttcaa gccagatata tttgtccgcg
     4021 ggatcatcgg agtgggactg cgcggaactg aaagccgtgc cgaaagcgag gcaggcaagg
     4081 gcaagcgaag cgacagaaaa ggcacggcca tgcggcatgc gaaaagctcc tgttggacag
     4141 tgcctgccat tctaaacagc cccgccgcaa aagaagaagg cgctccgcga gggagcgcct
     4201 tctgtgacgg ttaacctttc agacgggatg aaacgtcaga accggaactg cggtccaacc
     4261 gtgacgcgca gattgttgta gacgtcgttg ttcaggtaga cctcatctcc ggcttcaagc
     4321 cgcagcccga ccggacccca gaagccttcg acgccgcctc ccggataagc cgcgaagtgc
     4381 gtgccggaac caccaacgct ggcgaccgca tccgccgcct gctggcccgt tacattgtcc
     4441 gggttggtcg tggtgaagtt gatgaagccc accttgccgg tcacgaacgc atgaaagttg
     4501 ccaggaccaa agtggaaaac cgggccgaac agcccggtca gggggcgaac cccggtcttc
     4561 acaaacgtcg tcgtcgtgct gctccctgca gaattcgtaa ccgttgtggt gatattccgg
     4621 gcgaagtcgt agttcatctc ggcttccagc cccatatggt gatacgcgaa gcccacccgc
     4681 ccgccggctc ctacgaaatt ggtggacgtg ccgccccccg gagcaaagcg gaagtagtcg
     4741 gcatacacgc cgaactcaac ctgattgtcg cggccgtttt cctgcgccgc cagcaacgac
     4801 ggcagcgcca cgaagaggcc catccctata agcaat
//
LOCUS       DASZYN010000145         1889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_134659, whole genome shotgun sequence.
ACCESSION   DASZYN010000145 DASZYN010000000
VERSION     DASZYN010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134659"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            595..>1889
                     /locus_tag="VHE33_03795"
     CDS             595..>1889
                     /locus_tag="VHE33_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVT96603.1"
                     /translation="MQLAESRASRIWGFLSRGSPRAVLTRAAILIAATAILDWRTVDE
                     IPLRFLYLLPMLLVGSALKPLEIGGVAALCTFLAEIFDGFLWSPRTGISRDLLYFAAY
                     LAAGLFIREVKRNRSIVQQHLEELQRETEARREAEEQLKILIESSPAAIFTTDALGNM
                     LMANEAAHRMLGVASGGLPGRSIHRYLPSLRNVASSENAPQLYRSVMQARGQRDDGET
                     FLADISFSTYQTGAGTRLAAMVLDASEEFRSHEVSGLHQLLAGSRIAVGAVSHEIRNF
                     SGAIAAVHHNLSREGVLAGNRDFEALGNLIAALERIASINMQPANSQASEIDLGALLD
                     ELRIVIAPMLEEEEIISHWTIDPSLPMVWADRASLMQVFLNLVTNSIRALSSRDRRLF
                     SVTAGTEGERVVVEFADSGGGVLHPEQLFRPFQEGAQSSG"
BASE COUNT          388 a          566 c          552 g          383 t
ORIGIN      
        1 aacggaagca cacacgaaag ggctacagga ctacccggaa tggggatcca tctcgccgcg
       61 aagaagcaca tataatcgtg ggcttatcct ggcctgcgtt tcattgtcat ggagaagggc
      121 cgcgtggcag ccctgcaggg atagctgatc ctcacgtcgc gatgcgcccg gatgctgtat
      181 acgcaggatg attcagtctt tctgctccgg gacagagccg gcaactacgc caccaacgta
      241 accacccgga gcgccctccc tgcgcgcctg aaccggggaa aacctgggac gaagcgaatc
      301 tggaaaacga aagtaatcgc taccttcgca accctgtgca aacgactacc tatttcctca
      361 ttcgcgatgt cgcgtccccg ttatcgccgc cgcgcacaca agctgtcgtt cccactcgcc
      421 tggcgaacag gcattgccaa aaatcagcca gtccaggaat gcggcaaccg ccttctgact
      481 ccgagggcgg gcggcggtgc ccgtctgctt tgaccaggtc acgccgggag acgccagagt
      541 tcgttgtaag ctgagtctcg ttggttgcgc cgaaaactta caatcgaaac gaatatgcaa
      601 ctcgcagaga gtcgtgcgtc gcggatctgg ggattccttt cccgcggaag cccacgcgcc
      661 gtcctcacgc gcgcagcgat cctgatcgca gcaaccgcga tcctggactg gcgtacggtg
      721 gacgaaattc cgctgcgttt tctttatctg ttgcccatgc tgctggtggg gagcgccctc
      781 aagccgctcg aaatcggcgg ggtagcggct ctctgcactt tccttgctga gatctttgac
      841 ggttttctgt ggagcccgcg cacaggaatt tcacgcgatt tgctctattt tgcggcgtat
      901 ctggctgccg gactcttcat ccgcgaggtc aaacggaacc gcagcatcgt acagcaacac
      961 ctcgaagagc ttcagcgcga gaccgaagca aggcgtgaag ctgaagagca gctgaagatc
     1021 ttgatcgaga gcagcccagc ggctatcttc actactgatg ccctgggcaa tatgctcatg
     1081 gcgaatgagg ccgcgcatcg catgctcggc gtggcttctg gaggactgcc cggtaggagc
     1141 atccaccgct acttgccctc gttgcgcaat gtcgccagct cggagaacgc cccgcagctt
     1201 taccggagcg tgatgcaggc acggggccag cgggacgatg gagaaacctt tctcgccgac
     1261 atctccttct ccacatacca aaccggcgcg ggaacgaggc tggccgccat ggtgctggat
     1321 gcgtcggagg aattcaggag ccacgaagtc tcgggactcc atcaattgct ggctggatcg
     1381 cgcattgcag tcggagcagt ttcgcatgag atacgaaact tctccggagc gattgccgcc
     1441 gtccaccaca atctctctcg cgaaggtgta ctcgccggca atcgggattt tgaggctttg
     1501 ggcaatctga ttgctgccct ggagcgaatc gcaagcatca acatgcagcc ggccaacagt
     1561 caggccagcg agatcgatct cggagcgctg ctggatgaat tgaggattgt gattgctccg
     1621 atgctcgaag aagaggaaat catctcccac tggaccatcg acccttccct gcccatggtg
     1681 tgggccgacc gagccagcct catgcaggtg ttcctcaatc tcgtcaccaa cagtattcgg
     1741 gcactctcct cacgggaccg gcgcctcttt tccgttaccg ccggaacgga aggcgaacgt
     1801 gtggtggtgg agtttgccga cagcggcggt ggggttctgc atccagagca gttgtttcgt
     1861 ccttttcagg agggcgcgca atcgtccgg
//
LOCUS       DASZYN010000146         4016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_841873, whole genome shotgun sequence.
ACCESSION   DASZYN010000146 DASZYN010000000
VERSION     DASZYN010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841873"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /gene="prfA"
                     /locus_tag="VHE33_03800"
     CDS             <1..939
                     /gene="prfA"
                     /locus_tag="VHE33_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HVT96604.1"
                     /translation="KFREYRQVKTGIADARAMLNESDADIRAMAQDELTALEARQPQI
                     EEDLKLLLLPKDPNDEKNVVLEIRAGTGGDEASLFAAEIFRMYNRFAEQHRWRVEVLS
                     SSESSVGGLKEVIAIIEGDKVYSQMKYESGVHRVQRVPATETQGRVHTSAVTVAVLPE
                     AEDVDVKIEAKDIRIDTFCSSGPGGQSVNTTYSGVRITHLPTNTVVSCQDEKSQIKNR
                     EKGMRVLRARLYEMEMERQQQALAKERKQQVGSGDRSEKIRTYNFPQNRLTDHRIGLT
                     IHQLAEIMEGKLQPVIDALIAHFTAERLKTEAETVA"
     gene            980..1510
                     /locus_tag="VHE33_03805"
     CDS             980..1510
                     /locus_tag="VHE33_03805"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT96605.1"
                     /translation="MLIRNAVVNDARPIAQVHLASWKTTYPGLIPQEYIDGLRVEKGT
                     ADWQAALSSGRTTVLVAEDEKTVVGFAAGGAIAHAVDGYEGELGAIYLLASHQRRGIG
                     AALLRRMAAELQGKGLRNMAVWVLRENPACGFYRRLGGIQVGEQTIEIGGRVLPEIAY
                     GWPEIGVLCEDELAHG"
     gene            1517..2350
                     /gene="prmC"
                     /locus_tag="VHE33_03810"
     CDS             1517..2350
                     /gene="prmC"
                     /locus_tag="VHE33_03810"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HVT96606.1"
                     /translation="MRNLREIVDRAAARLGSSPTARRDAELLVMRAAGRDRAWLMTHP
                     EAELTASQTADLESWIARRARHEPLQYILGEAEFYGLTFRVTPDVLIPRPETEHLVEA
                     VLERVGQETAVRICDVGTGSGCIAVALAHGLRRAQVTALDLSRAALAVAKGNAERAGV
                     DGRVRFVESDLLRAVRGERFDVVVSNPPYVRDDEVLEAQVREHEPHAALFAGATGLEV
                     YERLIPEAHEALVPGGWLLMEIGHGQRDALARLLRSWDGVCFVPDLQGIPRVALAQRR
                     A"
     gene            2362..3624
                     /locus_tag="VHE33_03815"
     CDS             2362..3624
                     /locus_tag="VHE33_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HVT96607.1"
                     /translation="MSATAIETSTTTKDFATKLDRLAEVAIKCGLGLAEGQELVMTAS
                     TDALPLARLITKHAYAAGASLVTILFADEEAQLMRYRYGREGSFDAAAAWLYDGMAAA
                     YKSGAARLAITGGNPALLSKENPEHVSRANRAMSKAYRPALELITRHDINWTIVSAAT
                     PAWAAAMFPELPAEQALGKLWDAIFAASRADQDDPVAAWKKHDEKLHTWAERMNQRRY
                     AALHFKGPGTDLKVGLADDHLWLGGGTTAGNGNYCIPNMPTEEVFTTPHKDRVDGTVR
                     STKPLSHQGTLIEKIEVRFEGGKIVEAHAAKGEQVLQKLIETDDGARRLGEVSLVPNS
                     SPISASGLLFLNTLFDENAACHIALGQAYSSCLKNGDKLTPEELAAKGANDSLIHVDW
                     MIGSGQVDVDGITPTGGAEPVMRKGEWV"
     gene            3754..>4016
                     /locus_tag="VHE33_03820"
     CDS             3754..>4016
                     /locus_tag="VHE33_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96608.1"
                     /translation="MSNRRVSIFASVVLLGAGFFVPPTSVRAEAAPLTVEELVALGTN
                     ATFHTDLTFDESMLQAASQTLPDEDRPIVAKLRSIAVHTYRYSA"
BASE COUNT          801 a         1079 c         1449 g          687 t
ORIGIN      
        1 aagttccgcg aatatcgcca ggtgaagacg ggcattgccg atgcgcgcgc catgctgaac
       61 gaaagcgacg ccgacattcg cgccatggcc caggacgagc tgaccgcgct cgaggcgcgg
      121 cagccgcaga tcgaagagga cctgaagctg ctcctgttgc cgaaggatcc caacgacgag
      181 aaaaacgtcg tgctcgagat tcgcgcggga accggcggcg acgaggcgag cctctttgcg
      241 gccgagattt tccgtatgta caaccgcttt gccgagcagc atcgctggcg cgtggaggtg
      301 ctgtcttcgt ccgagtcgtc agtcggcggg ctgaaggaag tgatcgcgat catcgagggc
      361 gacaaagtgt actcgcagat gaagtatgag agcggcgtgc accgcgtgca gagggttccc
      421 gcgacggaga cgcaggggcg tgtgcacaca agcgcggtga ccgtcgctgt gctgccggaa
      481 gccgaggatg tggacgtgaa gatcgaggcg aaggacattc gcatcgatac attttgttct
      541 tcagggccgg gcgggcagtc cgtgaacacg acctactccg gcgtgcgcat cacgcatctg
      601 ccgaccaata ccgtggtgag ctgccaggat gaaaagtcgc agatcaagaa tcgcgagaag
      661 ggaatgcgcg tgctgcgggc tcgcctgtac gagatggaga tggagcggca gcagcaggcg
      721 ctggcgaaag agcgcaagca gcaggtgggc agcggggacc gcagcgagaa gatccggacc
      781 tacaacttcc cgcagaaccg gctcacggat catcgcatcg ggctgacgat tcaccagctg
      841 gcggagatca tggaaggcaa gctgcagccg gtgatcgacg cactgatagc gcactttacc
      901 gccgagcggc tgaagactga agccgagacg gtggcgtaaa cagcgcacag ttcactgggc
      961 gcagagaaag ggtcagtcaa tgctgatcag gaacgcggtc gtcaatgacg ctaggcccat
     1021 cgcgcaggtg catctggcga gttggaaaac gacctatccg gggctcattc cgcaggaata
     1081 catcgacgga ctgcgtgtcg aaaagggcac tgcggactgg caggctgcac tgagttccgg
     1141 ccggaccacg gtccttgtcg ctgaggacga gaagactgtg gttggttttg cggccggcgg
     1201 tgcgattgcg cacgccgttg acggatacga gggcgaactc ggcgcgatct acctgctggc
     1261 ttcgcatcaa cgtcgaggca tcggggcagc gctgctgcgg cgaatggccg ccgaactcca
     1321 ggggaaggga ttgaggaaca tggcggtgtg ggtactgcgc gagaatcctg catgcggttt
     1381 ctatcggcgc ctgggcggca ttcaggtggg cgagcagacg attgaaatcg gtggcagggt
     1441 gttgccggag attgcgtacg ggtggccgga gatcggggtg ctgtgcgagg acgagcttgc
     1501 gcacggctga atgcgtgtga ggaatctgag agagattgtg gacagggcgg cggcgcgtct
     1561 gggttcttcg cccacggcaa ggcgcgacgc ggagctgctg gtgatgcgtg cggcggggcg
     1621 ggatcgagct tggctgatga cgcatccgga ggcggaactg acggcttcgc agacagccga
     1681 cctggaaagc tggattgcgc ggcgggcgcg gcatgagccg ttgcaataca tcctgggcga
     1741 agcggagttc tatgggttga cattcagggt tacacccgat gtcctgatcc cacggccgga
     1801 gacagagcat ctggtggagg cggtgctcga gcgagtcggg caagagacgg cggtgcggat
     1861 ttgcgatgtg gggacggggt cggggtgcat tgcggtcgcg ctggcgcatg gactgcggcg
     1921 ggcgcaggtg acggcgctcg atctttcccg cgcggcgctc gcagttgcga agggaaatgc
     1981 ggagcgcgcc ggagttgatg ggcgcgtgcg gttcgtcgag tcagacctgc tgcgcgcagt
     2041 gcggggcgag agattcgacg tggtggtgtc gaatccgccg tatgttcgcg acgacgaggt
     2101 gctggaggcg caggtgcgcg aacacgagcc gcacgcggcg ctgtttgcgg gggcgacggg
     2161 gctggaggtt tatgaacggc tcattccgga agcgcatgag gcgcttgtgc cggggggatg
     2221 gctgctgatg gagatcggac acgggcagcg ggatgcgctg gcgcgattgc ttcggtcgtg
     2281 ggacggggtc tgcttcgttc cggacctgca ggggattccg cgcgttgctc ttgcacagcg
     2341 tcgggcctga gaatagagag catgagcgca accgcgatag aaacgagcac gacaacgaag
     2401 gattttgcca cgaagctgga ccgtctggcc gaggtcgcca tcaagtgcgg actggggctg
     2461 gcggagggcc aggaactggt gatgacggcc agcaccgatg cgctgccgct ggcgcggctg
     2521 attacgaaac atgcgtacgc agcaggcgcg tcactggtga cgattctgtt cgccgatgaa
     2581 gaggcgcagc tgatgcgtta tcgctacggg cgcgaaggca gcttcgacgc ggctgccgcc
     2641 tggctctacg acggcatggc ggcggcttac aagagcggcg cggcgcgcct cgcaatcacg
     2701 ggcgggaatc cggcattgct ctcgaaagag aatccggagc acgtgagccg cgcgaatcgc
     2761 gcgatgtcga aggcataccg gcctgcgctg gaactgatca caaggcacga catcaactgg
     2821 accattgtgt cggcggcgac tccggcatgg gcggcagcca tgtttcccga gttgccggcg
     2881 gagcaggcgc tgggcaagct gtgggatgcg atcttcgcgg ccagccgcgc cgaccaggac
     2941 gatcctgtcg ccgcctggaa aaagcatgac gagaagttgc atacctgggc ggaacggatg
     3001 aatcaacggc gttatgcagc cttgcatttc aaaggtccgg gaaccgatct gaaggtgggc
     3061 ctcgccgacg atcatttgtg gctgggcggc ggcaccacag caggcaatgg aaactactgc
     3121 attcccaata tgccgacgga ggaagtcttc acaacaccgc acaaagatcg cgtggatgga
     3181 accgtccgca gcaccaagcc gctttcccat cagggaaccc tgatcgagaa gatcgaggtg
     3241 cggtttgagg gtggaaagat cgtggaggca cacgcagcga agggcgagca ggttctccag
     3301 aagctgattg agaccgacga cggcgcgcgc aggctgggcg aggtgtcgct ggtgcctaac
     3361 tcgtcgccga tctcggccag cgggctgctg ttcctgaaca cgctgttcga tgaaaacgcc
     3421 gcgtgccaca tcgcgctggg gcaggcctat tcgagctgcc tgaaaaacgg agacaagctg
     3481 acgccagagg aattggcggc aaagggcgcc aacgacagcc tgatccatgt ggattggatg
     3541 atcggctcag gccaggtaga cgtggacggc atcaccccca ccgggggtgc ggagccggtg
     3601 atgcgcaagg gcgaatgggt gtaaagccgg agaattgcca atagagtaga accccggcgg
     3661 ccggatcgac cggctccaga agttgtcgat tgaactttcg aaaatcgccg atttcctccg
     3721 ccacttttcc ccggcaagcg cgttgaaggg tgcatgtcga atcgacgtgt ctccattttt
     3781 gcgtccgtgg ttctgctggg tgcgggcttc tttgttccgc cgacgagtgt gcgggctgag
     3841 gcggctccgc tgacggttga agaactggtg gccctgggga cgaatgccac gttccatacc
     3901 gatttgacct tcgacgagtc gatgctgcag gcggcgagcc aaactctgcc cgatgaggac
     3961 cggccgattg tcgccaaact gcggagcatc gctgtccaca cctatcgcta ctccgc
//
LOCUS       DASZYN010000147        10701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_134693, whole genome shotgun sequence.
ACCESSION   DASZYN010000147 DASZYN010000000
VERSION     DASZYN010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 10701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10701
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134693"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VHE33_03825"
     CDS             <1..580
                     /locus_tag="VHE33_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT96609.1"
                     /translation="SVALAMYISVMIGGGMLYIDDKARGVHEGYLVTPITKLELVLGL
                     NIAGAVKAVLSGISLSVIGSLLAGLGVVFHLGSVLQLALLIVATSIAFNGMMFLLMVR
                     VEDPLVPRAMFGVLNTLLFFPSGAIYPIEAFPKWLRAIAVVDPFTYAVHGFKAILIKD
                     GGFAAIQKDILFLFGFGIISLLLATPLFKRTL"
     gene            complement(988..3348)
                     /locus_tag="VHE33_03830"
     CDS             complement(988..3348)
                     /locus_tag="VHE33_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HVT96610.1"
                     /translation="MSSGTTRYAPQSDRVKLVVASSVMLTFISFWRAAAVVLNDLGSS
                     AFYAGGIAEQAVGKAAPWFILGVMLFSYAVRSVYVESSSMFTRGGVYRVVKEALGGNF
                     AKLSVSALMFDYVLTGPISGVSAGQYITGLLDQLLDFGALHHWLPASMMQNAHQAAHL
                     PMDGTSAVVALGVTIYYWWQNTKGIEESTDKALSVMQVTTVMVILLLGWGFITAFHRH
                     ASLPPLPTPSNLHFSSDALGFLRGTRFAQMLGLFGIVMAFGHSVLAMSGEETLAQVNR
                     EIEHPKLKNLRRAAIVIAVYTFLFTGIGTLLANMIIPDAVRVPVYRDNLIAGMAMYMW
                     GPLVIRIVFQVFVVIVGFLILSGAVNTSMIGSTGVMMRLAEDGVLTDWFRKPHRRFGT
                     SFRIINLITGLQLFVIIVSRGNVIMLGEAYAFGVIWSFTFNSLAMLVLRWKHKEERGK
                     KVPINLKIGGTELPLGLFFVFLTLLTVAVVNLVTKSMATESGIAFTAVFFTVFVISER
                     RNAGKHQLTQQQMKEHFQLEQQETIGREALQVRPGGVLVTMRDPVNPYALKWALSRTR
                     TDQQDIVVLAARMMGAGGPEYVEDQIFSEHEQMLFTKAVSVAESFGKHISLLVVPAGD
                     IFAALVQAANSLEVAALISGLSTRMSAQEQAFHIGQAWENLSEPKRQFTFYVVMPDGE
                     AEAFHIGPHAPALRAEDVQLVHRLWLNFRRDPEMQGLHHSDIMTYALTRLAMDYARDK
                     QETLRSLRRYTQEGGRDTQLGISYRGPGHGDQSIPAPRPSLDEEDH"
     gene            3585..4838
                     /locus_tag="VHE33_03835"
     CDS             3585..4838
                     /locus_tag="VHE33_03835"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVT96611.1"
                     /translation="MTDDAPLRSLTALLGTPVTSVRGRMRGRLKDVAVGTGEEAGRVA
                     GLVVKTRKGLEMVSSKDVRWTPSGALELRPEATLNPLQGYENFILLRQDLLDRQIIDV
                     HGRKVVRVNDVNLRWQPSDDGEQLCVLEVEVGLRGAVRRLFNGALPKRTLENLVAKLP
                     PRVIPWNFVDMIEVDPARRVKLNIEHERLAQLHPSDIADILEDLAPAEREAVLRTLDE
                     EVAAETLEEVEPKLQKSLVQALDSETAAGIVEEMDPSAAADLLAELSEERSEAILEEM
                     DPEERQEVKELLEFREDSAAGRMTTEYVAVPQSATVADAIRALRGFEGDPETITEIYL
                     IDSGEVLKGVVPLARLVLARPETRLEVLADPRFISVQFDQHENEVAELFDKYNLRALP
                     VVDKNGKLVGAVEADHVIAFLRSRV"
     gene            complement(4835..5761)
                     /gene="mdh"
                     /locus_tag="VHE33_03840"
     CDS             complement(4835..5761)
                     /gene="mdh"
                     /locus_tag="VHE33_03840"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HVT96612.1"
                     /translation="MRKKVTIVGAGNVGATAAHWIAGKELADVVLIDVVEGIPQGKGL
                     DLLEAMPIAKTDCHILGTNDYADTANSDIVVITAGIPRKPGMSRDDLLQTNYKIMSDV
                     VGKVTAASPNCILIIVSNPLDAMAQAAFRQAKFNRERVIGMAGVLDSARFRTFIAEEL
                     KVSVENVTAFVLGGHGDTMVPLARYSTVAGIPITELIEKEKLDAIIQRTRDGGAEIVK
                     YLKTGSAYYAPSAATVEMVEAILKDKKKILPCAAYLEGEYGIRGYYIGVPCKLGSRGI
                     EQIVEIKLTPEEDAALKKSADAVKELCAVIGV"
     gene            complement(5834..7234)
                     /locus_tag="VHE33_03845"
     CDS             complement(5834..7234)
                     /locus_tag="VHE33_03845"
                     /inference="COORDINATES: protein motif:HMM:NF005610.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HVT96613.1"
                     /translation="MQTTYNGLTLPKDGEAIQYANGKYTVPDRPIIPFIEGDGTGRDI
                     WRASQQVFDAAVEKSYGGKRAVKWFEIFAGEKAFREFKTWLPDDSVKAATDLRVSIKG
                     PLTTPVGGGIRSLNVALRQIMDLYACVRPVKYYAGVPSPVKRPEKLDVVIFRENTEDV
                     YAGIEFKEGTADAKKLIGFLNDEMLKGGKKKVREDSGVGIKPISITGTKRLVRYAIRH
                     AIATGRKSVTLVHKGNIQKFTEGAFREWGYEVAVQEFRDQTVTERESWILGNVEANPG
                     LSVEQNAALIEPGLEFGNDEFRQSLYAEIKGVLDSIGKSHGNGQWKKKVMINDRIADS
                     IFQQVIIRPEEYSILACPNLNGDYISDACAAQVGGLGIAPGANIGDGYAVFEATHGTA
                     PKYADKDVINPGSVMLSGVMMFEFLGWPEAAKLIETSMEKTIQQKYVTYDFERQMQGA
                     TKVKTSEFATKMIANM"
     gene            7365..8474
                     /locus_tag="VHE33_03850"
     CDS             7365..8474
                     /locus_tag="VHE33_03850"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HVT96614.1"
                     /translation="MLLRARLFLLLGVGLIASMPLSAQPAPVRVAIVGLEHGHVSGFL
                     HQFPKQNETQLVGIVDADSALCAKYEQQFHLDHGLFYATLDDLFAHQQPQALLVYTSI
                     AQHRRVIEAAAAHSVTVMVEKPLTLSLADAIAIRRIAHEHHIQVLVNYETTWYPSNRA
                     LYDLLRHDSLGDLRKAVIHDGHQGPKEIGVQPEFLQWLTDPEQNGAGALYDFGCYGAD
                     LMTWFMHGETPVSVTAVAQTDKPETYPRVDDDATIIVRYRNAQAVLMPSWNWPFSRKD
                     AEVYGTKGYAIAPNPTQIRERVNPASPEVAIGIPPLPAPSDTSLHYLAAVVRGQLDPD
                     HDLSSLDTNMVVMQILDAARQSAKTGKTVPLHPLP"
     gene            8503..9375
                     /locus_tag="VHE33_03855"
     CDS             8503..9375
                     /locus_tag="VHE33_03855"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HVT96615.1"
                     /translation="MIRLRIPVAALTISGILSLGALTAHAQYSADRPVGTSAQQAPAW
                     QKNAGIDQNLNRPLPLGDSFQDESGRDVKLGDFFHDQRPVMLALMYYNCQMLCPQVLH
                     GMASGLRESGFHAGHEYDVVVASIDPSDKPTDAVSEKQHFLSMLDDSSSTAANAVHFL
                     TGGQTSIADLAQATGFHYVRVPGPDGRMNQFAHSSVIMIVTPDGRMSKYLFGVDYQSR
                     DVRLALIQASSRHIGTLSDLVLLYCCSYSPTQGRYTVAILRIMGLAGVASLLAVIAML
                     GLLSKKPKNTPVGV"
     gene            9447..10358
                     /locus_tag="VHE33_03860"
     CDS             9447..10358
                     /locus_tag="VHE33_03860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HVT96616.1"
                     /translation="MRAVVLYEYGDVDKLRFENDQQEPPFGPNEVRVRVRATSINPVD
                     WKIRSGSARQRIPVDFPAILGRDLAGEVVDFGEQVTGFSKGQRVMGLANGTYAEFTTA
                     KADVLAPIPDSLSFEQAAALPLVLTTGAQLIERAIKPQTGWTVLVTGAVGGVGRTAVH
                     VARKHGVHVIAGVKSSQRDEASSLGADRVIALDDESDIASLRNLDAIADTVGGATIAI
                     LLKAIRPGGVLGSVVGEPKEAAGKNIQVHAFMAQPDASRLYELADEAARGEFTIPIAR
                     TFRLDQAREAQDLAEHKHPGGKVLLVA"
     gene            complement(10355..>10701)
                     /locus_tag="VHE33_03865"
     CDS             complement(10355..>10701)
                     /locus_tag="VHE33_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HVT96617.1"
                     /translation="ARDGLSSARAVIVCAGMEGALPSVVGGLVAAPVIAVPTSVGYGA
                     AVGGVAALLGMLNSCSPNVCVVNIDNGFGAAYVAAMMVRAQGAEPTAQSRESEAAEKD
                     NGARSAASCYRR"
BASE COUNT         2113 a         3378 c         3052 g         2158 t
ORIGIN      
        1 ctcggtcgcc ctcgccatgt atatctcagt gatgatcggc gggggaatgt tgtatatcga
       61 tgataaggcg cggggggttc acgaaggtta tctggtcacg ccgatcacga aacttgaact
      121 ggtgctcggc ctcaacatcg caggcgccgt gaaggcggtg ctgtccggga tcagtctgtc
      181 ggtgattggt tccctgctgg ccgggctggg cgttgtcttc cacctcggat cagtacttca
      241 gctggcgctt ctgattgttg ccacgtccat cgcgttcaac ggcatgatgt ttctgctgat
      301 ggttcgggtt gaagatccgc tggttccacg agccatgttt ggagtgctca atacgctgtt
      361 gttcttcccc agcggagcga tctatccaat tgaagcattt cccaaatggc tgcgggcaat
      421 cgctgtcgtc gatccattca cctatgcggt tcatggtttc aaggccatcc ttatcaaaga
      481 tggcgggttt gcagccatcc agaaggacat cctgtttctt tttgggtttg ggataatttc
      541 cctgcttctg gccacgccgc tcttcaagcg cacgctttag cggtaagggc ggggagggtc
      601 ccgccccgtt ttacacggct ttggactttt gtcctgtgga tttgcgccgg agggcccaac
      661 ggcgcttcag cgcctcagaa atccgccttc tcccctcagg ggtgaggtga cgtttgcgtc
      721 ctgccggctg agtccgtgat ttgcccggag gtcgtccgcg tcttttcggc cctcccgccc
      781 catcggttaa caaggcgcga gcttgcctca gccgggcaat ttggtcgtcc aactccgata
      841 cgatcttgct gagttccaca ttatcctccc gtttcggatc ctgaagcgca ccctgcacac
      901 cgaaagcaag gcaggcttat tgattgtagt ttgagactaa acgggaaggg atatctttac
      961 aaacccggac tgaatagaag ggcgaggtta gtggtcctct tcgtcgagtg agggtcgcgg
     1021 tgcgggaatg gactgatcgc cgtggccggg acctcgataa cttattccca gctgtgtatc
     1081 acggccgcct tcctgggtat accggcgcag gcttctgaga gtttcctgct tatctcgcgc
     1141 atagtccatg gccagccggg tgagcgcgta ggtcatgatg tcgctgtgat ggagtccctg
     1201 catctcggga tcgcgacgaa aattcagcca taaacgatgg accagctgga catcctcggc
     1261 acgcaatgcc ggtgcgtggg ggccgatatg aaaagcttcg gcctccccgt ccggcataac
     1321 cacatagaag gtgaactgtc gcttgggttc ggagagattt tcccacgcct gtccgatatg
     1381 aaaggcctgt tcctgcgccg acatacgagt ggataagccg gaaatgagag cagcgacctc
     1441 gagcgaattc gccgcctgca ccagcgccgc gaagatatcg cctgccggaa cgaccagcag
     1501 ggaaatatgc tttccgaagc tctcggcgac ggagaccgcc ttggtaaaga gcatttgctc
     1561 gtgctcgctg aatatctgat cctcgacgta ctccgggccg ccggctccca tcatcctggc
     1621 ggccagcacg acaatatcct gctgatcggt ccgtgtacgc gacagggccc atttcaaagc
     1681 gtagggattc accggatcgc gcatcgtgac cagcacgccg ccgggacgca cctgcagggc
     1741 ttcacggccg atcgtttcct gctgctcgag ctggaaatgc tccttcatct gctgctgcgt
     1801 cagctgatgc ttgcctgcgt tgcggcgttc agagataaca aaaacggtga agaaaaccgc
     1861 tgtaaaagcg atgccgctct cggtggccat actcttggtt accagattca ccacggcgac
     1921 cgtgagcagg gtgaggaaga cgaagaacag gccgagcggc agttcggttc cgccgatctt
     1981 cagattgatg gggactttct tgccccgttc ttctttatgc ttccagcgca gcaccagcat
     2041 agcgagggag ttgaaggtaa agctccagat cacgccaaag gcataagctt cacccagcat
     2101 gatcacgtta ccgcggctaa cgatgatgac aaagagctgc agtccggtaa tgagattgat
     2161 gatgcggaaa cttgtgccga aacggcgatg gggcttgcgg aaccagtccg tcaacactcc
     2221 gtcttcagcc aggcgcatca tcacgcccgt cgaaccgatc atcgacgtgt tcaccgctcc
     2281 ggaaaggatc agaaatccga caataacgac gaagacctga aagacgatgc ggatgaccag
     2341 aggtccccac atatacatcg ccatgccggc aatcagattg tcgcggtaaa ccggcacgcg
     2401 gactgcgtcg ggaatgatca tgttcgcgag cagcgttccg ataccggtga acaggaacgt
     2461 gtagaccgcg ataacaatgg cagcgcggcg cagattcttg agtttggggt gttcgatctc
     2521 gcggttcacc tgggcgagtg tttcttcgcc gctcatcgcg agcacggagt ggccaaaggc
     2581 catcacgatc ccaaacaagc ccaacatctg cgcaaagcgc gtgccgcgca ggaatcccag
     2641 ggcgtcggat gaaaagtgga gattggacgg ggtgggcagc gggggcaacg aggcatgccg
     2701 gtggaacgct gtgataaagc cccagcccag cagcaggatc accataactg tcgtgacctg
     2761 catgacgctc aacgctttgt cggtggactc ttcgatgcct ttggtgttct gccaccagta
     2821 gtaaatggtg acacccaaag caacaaccgc tgaggttccg tccatgggca ggtgggcggc
     2881 ctgatgcgcg ttctgcatca tcgacgccgg tagccagtgg tgcagtgccc cgaagtcaag
     2941 caactgatcg agtaaacccg tgatgtactg gccggccgaa acgcccgaga tcggccccgt
     3001 cagcacgtaa tcaaacatca gcgcggagac gctgagtttg gcgaaatttc cgcccagggc
     3061 ttctttcacc acgcgataga cgccgccgcg agtgaacatc gaggagctct cgacgtacac
     3121 ggagcgaacc gcataggaaa aaagcatcac cccgaggatg aaccagggag cggcctttcc
     3181 gacggcttgt tcggcgatgc ctcccgcgta gaaggcggag gatcccaggt cgttgagcac
     3241 caccgcggcc gcacgccaaa aggagataaa cgtgagcatc accgaggaag cgacgaccag
     3301 cttcactcgg tcggactgcg gcgcgtaccg ggtggttcct gaggacattc gaggcgttga
     3361 gcttcaacgg cggccgcggc aacgttgctg cctccgccga cgcgcccagc gctgagtgta
     3421 tgtccgtatc gtgccgcagt caatcagaac tgttcgtgtg gggccatttt gtgacaggca
     3481 aatctgcgca tctgattgag tcagcggagc taatgcattg atttgcacgg ctgccggagt
     3541 atcggaaaaa tgggcgctgg tacactcttg ccgagaccgg aatcatgact gacgacgcgc
     3601 cgctacggtc ccttaccgcc ctcctgggga cgcctgtaac gagcgtgcgt ggacggatgc
     3661 gcggccgcct gaaagacgta gccgttggca ccggcgagga agcaggccgc gtcgccggtt
     3721 tggtggtgaa gacgcgcaag ggcctggaga tggtgtcctc caaagacgtt cgctggactc
     3781 ccagcggcgc tctcgaactg cgcccggaag cgaccctgaa tccgctgcag ggatacgaaa
     3841 atttcatcct tctgcgccag gaccttctcg accggcaaat catcgacgtg catggcagga
     3901 aagtcgttcg cgttaacgac gtcaatctgc gctggcaacc gagtgacgac ggggaacaac
     3961 tctgcgtcct cgaggtggag gttgggctgc gtggcgccgt gcgcaggctg ttcaacggtg
     4021 cgctgcccaa acgcactctg gagaatttgg tcgccaaact tccgccccgc gtgattccct
     4081 ggaattttgt cgacatgatc gaagtcgatc cggcgcggcg cgtcaagctg aacatcgaac
     4141 acgaacgcct ggctcaactg cacccatcgg acatcgccga cattctggaa gacctggcgc
     4201 cggcggagcg ggaagcggtg ctgcgcaccc tcgacgagga agtggcggcc gagacgctgg
     4261 aggaagtcga gcccaaactc cagaagtctc ttgtgcaggc tctggattcg gaaaccgccg
     4321 ccggcatcgt ggaagagatg gatccctccg ccgccgccga tctgctcgct gaactatcgg
     4381 aggagcggtc ggaggccatc ctcgaagaga tggatccgga ggagcgccag gaggtcaagg
     4441 agctccttga attccgcgaa gactccgcgg ccggtcgcat gaccaccgag tacgtggcag
     4501 tgccgcagag cgccaccgtc gctgatgcaa ttcgtgccct gcgcgggttt gagggcgatc
     4561 cggaaacgat cactgagatc tatctgatcg attcaggcga agtcctgaag ggagtggtgc
     4621 cgttggcccg cctggtgctg gcccggccgg aaacgcgcct ggaagttctg gccgacccgc
     4681 gcttcatttc ggtacagttc gaccagcacg agaatgaggt ggcggaattg ttcgacaaat
     4741 acaatctgcg cgcgctgccg gtggtcgaca aaaacggcaa actggtcggc gccgtggaag
     4801 ccgaccacgt gattgccttc ctgcgctccc gggtttagac gccgatcacg gcgcacagct
     4861 cttttacagc atcagcgctc ttcttcaggg cagcatcctc ttccggcgtg agtttgatct
     4921 cgacgatctg ctcgatcccg cgcgagccca gcttacacgg cacaccaatg tagtagccgc
     4981 ggataccgta ctcaccctcc agataggcag cgcagggcag aatcttcttc ttgtccttga
     5041 ggatcgcttc caccatttca accgtcgccg ccgaaggcgc gtagtaggcg cttcctgtct
     5101 tcaggtactt cacgatctcg gcgccaccgt cgcgggtccg ctgaatgatc gcgtccagct
     5161 tctctttctc gatcagttca gtgatgggaa tcccggccac cgtggagtag cgtgccaggg
     5221 gcaccatggt atcgccgtgt ccgcccagca caaaggccgt aacattctcc accgatacct
     5281 tcagttcctc ggcaatgaag gtccgaaagc gcgcggaatc gagcacgccc gccatgccga
     5341 tgacgcgctc gcgattgaat tttgcctgcc ggaatgcggc ctgcgccatc gcatccagcg
     5401 gattggaaac aataatgagg atgcagttgg gcgatgccgc cgtcaccttc cccaccacgt
     5461 ccgacatgat cttgtagttc gtctgcagca gatcgtcccg gctcatgccc ggcttgcgcg
     5521 gaataccggc ggtgatcacg acaatgtcgg agttcgcggt atcggcgtag tcgttggtgc
     5581 cgaggatgtg gcagtcggtc ttcgcaatcg gcatggcctc cagcagatcg agacccttgc
     5641 cctgcgggat gccctcgacg acgtcgatca ggacgacgtc ggccagttct tttcctgcga
     5701 tccaatgagc cgccgtcgcg cccacattcc cggcaccaac gattgttacc ttcttccgca
     5761 taacgctcct tatcttctca atccattgac cgcggggctg cgccgaaggc gcgtatgccg
     5821 gtacggccag tcttcacata ttggcgatca ttttggtggc gaactcgctc gtcttcacct
     5881 tcgtcgcccc ctgcatctgg cgctcgaaat cgtaggtcac gtacttctgc tgaatcgttt
     5941 tctccatcga ggtttcgatc agcttcgcgg cctcgggcca gcccagaaat tcgaacatca
     6001 tgacgccgga taacatcacc gacccagggt taatcacatc cttgtcggca tacttgggcg
     6061 ccgttccgtg cgtcgcttcg aacaccgcgt agccgtcgcc gatattcgcg cccggagcaa
     6121 tccccagccc acccacctgc gccgcgcacg cgtcggaaat atagtcgccg ttcaggttcg
     6181 gacacgccag gatgctgtac tcctcgggcc ggatgatcac ctgctggaag atcgaatcgg
     6241 caatgcgatc gttgatcatc acctttttct tccactgtcc attgccgtgg gacttgccga
     6301 tcgagtccag aacacccttg atttcggcat acaacgactg gcgaaactcg tcgttgccga
     6361 attcgagtcc gggctcaatc aatgcggcat tctgttccac gctcagcccc gggttcgctt
     6421 cgacgttgcc gagaatccaa ctctcacgtt ccgtcaccgt ctgatcgcga aactcctgta
     6481 cggccacttc atagccccat tcgcgaaaag caccctcggt gaacttctga atatttccct
     6541 tatggaccag cgttacgctt ttgcgcccgg tagcaattgc atgacgaatc gcatagcgca
     6601 cgaggcgctt ggttccggtg atggaaatcg gcttgatccc cacgccggaa tcctcgcgca
     6661 ccttcttttt gccgcccttc agcatctcgt cattcaaaaa tccgatcagc ttctttgcgt
     6721 cggccgtgcc ctctttgaac tcgatgcctg cgtatacgtc ctcagtattc tcgcggaaga
     6781 tcacgacatc cagcttctcc ggacgcttaa cgggcgaagg cacgcccgca tagtacttga
     6841 ccgggcgcac gcaggcgtac aaatccataa tctgccgcag cgccacattg agagaacgga
     6901 tgccgccgcc caccggcgtc gtgagcggtc ccttgatcga gacacgcaga tccgtcgccg
     6961 ctttgacgga atcgtcgggc agccacgttt tgaattcgcg gaacgccttc tcgccggcaa
     7021 aaatctcgaa ccacttcacc gctcgtttgc cgccataaga cttttccact gccgcatcga
     7081 aaacctgctg tgaggcgcgc cagatatctc gcccggtgcc gtcgccttcg ataaagggaa
     7141 tgatggggcg atccggaacg gtgtacttcc cattcgcata ctggattgcc tcgccgtctt
     7201 tgggcaaagt caacccgtta taagtggtct gcatgtgtgt tgagttcctt agctgatggg
     7261 cctggttgat ggatcgttgc ggaagagtcg aattcccaag ctaacactac cgaaaccggc
     7321 gttgcaacgg gccgtccacg ctggcgcgcg cgtagcatac acctatgttg cttcgtgcgc
     7381 ggctcttcct gctcctcggg gtcggactca tcgcctccat gcctctttcc gctcagcccg
     7441 cgcccgtgcg cgtggccatc gtaggtttgg agcatggcca tgtctcggga ttcctgcacc
     7501 agttcccgaa acagaatgag actcaactgg tcggcatcgt cgacgcagac tcggccctgt
     7561 gcgcgaaata cgagcagcag ttccatctgg atcacgggct tttctacgcc acgctcgacg
     7621 acctgttcgc tcatcagcag ccgcaggccc tgctcgtcta cacctcgatt gcccagcatc
     7681 ggcgcgtgat tgaagcggct gccgcccaca gcgtgacggt catggtcgag aagcccctga
     7741 ctctctcgct cgccgacgcc atcgccatcc gccgcatcgc ccacgagcac cacatccagg
     7801 tcctcgttaa ttacgagacc acctggtacc ccagcaatcg cgccctctac gacctgctcc
     7861 ggcacgacag cctcggcgat ctgcgtaagg ccgtcattca cgacggacat cagggtccca
     7921 aagaaattgg cgttcagccg gagtttctcc aatggctgac ggatcccgaa cagaacggcg
     7981 ccggcgcgct ctacgacttc ggctgctatg gcgccgacct catgacctgg ttcatgcacg
     8041 gggagacgcc ggtgagcgtc acagccgtcg ctcagaccga caagcctgag acctatcctc
     8101 gcgtcgatga cgatgccacc atcatcgtgc gctaccggaa cgcgcaggcg gtgctgatgc
     8161 cctcctggaa ctggcctttc agccgcaagg acgccgaagt ctacggcacg aaagggtacg
     8221 cgatcgctcc caaccccacg cagattcgtg agcgcgtgaa tcccgcctcg cccgaagtcg
     8281 cgatcggtat cccgccgctt cccgctccca gcgatacttc cctgcactac ctggccgcgg
     8341 tggtgcgagg gcagctcgac cccgaccacg acctcagttc cctcgacacc aacatggtcg
     8401 tcatgcagat tctcgacgcc gcccgccagt ccgcgaagac gggaaaaacg gtgcccctgc
     8461 acccgctgcc ctgaagcccg acgtcgcggc gtattctgga atgtgattcg ccttcgcatc
     8521 cccgtcgcag cacttacgat ttccggaatt ctttcccttg gcgccctcac ggcgcacgct
     8581 cagtactccg ccgatcgccc ggtgggcacg agtgcccagc aggcgccggc ctggcaaaag
     8641 aacgcaggca tcgatcagaa tctcaatcgg cctcttccgc tgggcgactc gttccaggac
     8701 gaatccggcc gcgatgtcaa actcggcgac ttctttcacg accagcgacc ggtgatgctg
     8761 gctctgatgt actacaactg ccagatgctc tgcccccagg tgctgcatgg catggcctcc
     8821 ggcctgcgcg agagcggctt ccacgccgga cacgaatacg atgtggtggt cgcgagtatc
     8881 gatccctccg acaaacccac cgacgccgtt tccgaaaaac agcacttcct ctccatgctg
     8941 gacgactcca gctcgactgc cgccaatgca gttcactttc tcaccggcgg gcagacctcc
     9001 attgcggatc tcgcccaggc aacggggttc cactacgttc gcgttcccgg ccccgacggc
     9061 aggatgaatc agttcgcgca ctccagcgtg atcatgatcg tcacgcccga tggccgcatg
     9121 tcgaaatatc tgttcggcgt cgattatcag tcgcgcgatg tccgcctcgc cctcatccag
     9181 gccagctcgc gccatatcgg cacgctcagc gacctcgttc tgctgtattg ctgcagctac
     9241 tcgccaaccc agggccgcta taccgtcgcc attctgcgca tcatgggcct tgccggcgtc
     9301 gcatcgctgc tggcggtcat cgcaatgctc gggctgctct caaaaaagcc gaaaaacacg
     9361 cccgtcggcg tctgaatcca gctcgaccga tttacaacaa gcgaccctgg gccattcccc
     9421 ttcatctcac aggaagaggt gctggcatgc gagcggttgt tctttatgaa tacggcgacg
     9481 ttgacaagct tcgttttgaa aacgatcagc aggaacctcc cttcggcccg aacgaggtcc
     9541 gtgtccgcgt gcgcgcaacc agcatcaatc ccgttgactg gaagatccgc agcggatcgg
     9601 cgcgccagcg catacccgtc gatttccctg ccatcctcgg ccgcgatctg gccggcgaag
     9661 tcgtggactt cggtgagcaa gtcaccggct tcagcaaggg ccagcgcgtg atggggctgg
     9721 cgaatggaac ctatgctgaa ttcacgaccg ctaaagccga cgtcctcgct cccatacccg
     9781 actccctcag cttcgagcag gccgcggcac tgcccctggt gctgacgacc ggcgcacaac
     9841 tgatcgagcg tgccatcaaa ccccaaacag gctggaccgt cctggtcacc ggcgccgtgg
     9901 gcggcgtcgg ccgtactgcc gttcacgtcg cgcgcaagca cggtgttcac gtgatcgccg
     9961 gcgtcaaatc ctcgcagcgc gacgaagcct cctcgctggg tgcggaccga gtgatcgccc
    10021 tcgatgacga aagcgatatc gcgagcctgc ggaacctcga tgccattgca gataccgtgg
    10081 gtggagcaac cattgccatc ctgctcaaag caatccggcc cggtggcgtg ctgggatcgg
    10141 tggttggcga gccaaaggaa gcggcgggaa agaacatcca ggtgcatgcc ttcatggccc
    10201 agcccgatgc atcgcggctt tacgaactgg ccgacgaagc cgcgaggggc gagttcacca
    10261 tccccatcgc cagaaccttc cgtttggacc aggcgcgcga ggcacaggat ctcgccgagc
    10321 acaaacatcc cggcggcaaa gtcctcctcg tggcttagcg ccgatagcaa gacgcggcgg
    10381 atcgggcacc gttgtccttt tccgccgcct ccgattccct gctctgtgcg gtgggctctg
    10441 caccctgtgc cctgaccatc atcgccgcaa cgtaggctgc gccgaatccg ttatcgatat
    10501 tcaccacgca cacgttcggc gaacacgaat tcagcatccc gagcagcgcc gcaacaccgc
    10561 cgaccgcagc cccgtaacca acactggtgg gcaccgcaat cacaggggct gccaccaatc
    10621 cgccgaccac gctgggcagc gcgccctcca tgcccgcgca gacaatgaca gcacgcgcgg
    10681 aactcaatcc gtctctcgcc g
//
LOCUS       DASZYN010000148         4223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_326019, whole genome shotgun sequence.
ACCESSION   DASZYN010000148 DASZYN010000000
VERSION     DASZYN010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326019"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..567)
                     /locus_tag="VHE33_03870"
     CDS             complement(232..567)
                     /locus_tag="VHE33_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
                     /protein_id="HVT96618.1"
                     /translation="MAGDLQLICSDCGQEFTFTSEDQAFFQERGYSAPKRCKSCRQAK
                     KTQMGGGGGGGGYQRAESRGTAVVCSGCGQQTTVPFEPRGDRPVYCRDCYQAQKGGAG
                     GGRGGRGRY"
     gene            754..1944
                     /locus_tag="VHE33_03875"
     CDS             754..1944
                     /locus_tag="VHE33_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVT96619.1"
                     /translation="MAFPFKGVDFIGFDSLLTDDERLTRDTARQFIEDNLVPIIEECN
                     RDGRFPKELVPQMGQLGFFGASLQGYGCAGMSNVEYGLVMQEFERGDSGVRSFVSVQS
                     ALVMYPIYTFGSDEQKNYWLPKMATGEKLGCFGLTEPDYGSNPGGMRTRADRSGDEYI
                     LNGEKMWITSGSIADVAVIWAKTYGDAGQEDGTVRGFLVECDRPGFKAYDVHGKWSLR
                     ASVTSGLSMQDVRIPATNLLPKSGGLKSPLMCLNQARYGIAWGGIGAAMSCYDTARQY
                     SLVRKQFRDQPIASHQLVQEKLAWMITEITKGQLLVLQVGRLKDQGKVEHQHISMAKR
                     NNIWMALECARMARDILGANGITDDFPVMRHMMNLESVKTYEGTHDIHTLILGQSVTG
                     IAAY"
     gene            2069..2728
                     /locus_tag="VHE33_03880"
     CDS             2069..2728
                     /locus_tag="VHE33_03880"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HVT96620.1"
                     /translation="MKLLSIQCGLPREVTWHGGRFTTSIFKEPVLGRIALRTLNLDGD
                     RQSDLTVHGGKYKAVYCYPVQHYEYWRRELPGRDLPWGAFGENFTIEDGPDEPSVHIG
                     DRFSIGSSEVVVTQPRFPCYKLGIKFQSDDMVRRFLASGRSGFYLAVTREGEVGAGDE
                     ITPLSHDPDGVSIADIIRLYIAKEFSDTDVRQLQKALVLEAFPDSWKDYLKEKMERLA
                     G"
     gene            2820..4037
                     /gene="opgC"
                     /locus_tag="VHE33_03885"
     CDS             2820..4037
                     /gene="opgC"
                     /locus_tag="VHE33_03885"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HVT96621.1"
                     /translation="MHFPKLQRLPELDALRGLFLVWMTLTHLPTHVSTFLNEPFGFVS
                     SAEGFVFLSAILIGRLYIRRSMEDPVGVRSHMWRRSFKLYAYHLFLLLFAFTLAASWA
                     VHHHRVAITNLLNFYLNHPGVAITGSIFLIYCPPLLDILPLYVMFLFLTPAFLSAAIR
                     WGWKTVLSVSALIWLGAQFGLRDVVHSAVVHLTHLQIPLQETGAFNLFAWQMVWIVGL
                     WLGACSAHRDVPLKLVPRWGVALSAAVCLFFVGVRFSWLGPHLTEQSLGLLLDKWQIG
                     PLRVLNIAAFLVVFYWLRRYIRVVVAREPFLTFGKASLQVFCAHLLFVFGGLALLVGE
                     MEQLTGWTAIALLAVTFVSLFLVAANEVRKKRRAKQGNPNPPNPGVPSLPPPDRPVNT
                     QPLAAVALEERPL"
BASE COUNT          760 a         1524 c         1107 g          832 t
ORIGIN      
        1 gcagaagcat cccaccgtca ccgagcaccc ggagcagcgt cgtcaggaag aaaccggcca
       61 cgaagacgcc agcgaccccg gtgaagtcgg tcggccgcct tcttcgagcg gcagccggcc
      121 ttccactaaa gagaaagacg ccgcctgatc tgccacactc cggcgaaggc gcgcgctcat
      181 ccctaagcgc gcatttcgct tgaagccgtc tcgtttttcg ctgatcggcg cttagtagcg
      241 cccgcgcccg ccgcgtccgc cgcccgcgcc gcccttctgc gcctggtagc agtcccggca
      301 gtacaccggc cggtcgcccc tcggttcaaa aggcaccgtc gtctgctgcc cgcaaccgga
      361 gcacaccact gccgtgccgc gcgactcggc tctctgatag cctccgccgc cgccaccccc
      421 gcccatctgg gtcttcttgg cctgacgaca gctcttgcag cgttttggtg ccgaataccc
      481 gcgttcctgg aagaacgcct ggtcttcgct cgtgaaggta aactcctgac cacagtcgct
      541 gcagatgagt tgcagatcgc cagccatcgg gttcctcgtt tctttcccct gctacgccat
      601 cattacaagg tttgtgttcc gcgtaaaccg tgatcgcaaa ttttgatcag acggcccgac
      661 ccggtttcgc tgcagtttag cgcgcacagg ccttccggtt ccagccttcc gccggaaccg
      721 gtatgatggc ccttccggag tgatttttct cgtatggctt ttcctttcaa gggcgtagac
      781 ttcatcggct tcgacagcct cctcaccgac gacgagcgcc tcacgcgcga caccgctcgc
      841 cagttcatcg aagacaacct cgtgcccatc atcgaggagt gcaatcgcga cggccgcttc
      901 cccaaagaac tcgtcccgca gatgggccag ctcggcttct tcggcgccag cctccagggc
      961 tacggctgcg ccggcatgtc caacgtcgaa tacggtctcg tcatgcagga gttcgagcgc
     1021 ggagactccg gcgtgcgcag ctttgtcagc gtgcagtccg cgctcgttat gtaccccatc
     1081 tacaccttcg gctccgacga gcagaaaaat tactggctgc cgaagatggc caccggagaa
     1141 aagctcggct gcttcgggct caccgagccc gactacggat ccaaccccgg cggcatgcgc
     1201 actcgtgcgg accgcagcgg cgatgagtac atcctcaacg gcgaaaaaat gtggatcacc
     1261 tccggctcca tcgccgacgt cgccgtcatc tgggccaaaa cctacggcga tgcaggccag
     1321 gaagacggca ccgtccgcgg cttcctggtc gaatgcgacc gccctggttt taaggcctac
     1381 gacgtccacg gcaagtggtc cctgcgcgct tccgtcacct caggtctctc catgcaggac
     1441 gtccgcattc ccgcaacgaa tctcctgccc aaatcaggcg gcctgaagtc gccgctcatg
     1501 tgcctcaacc aggcccgtta cggcatcgcc tggggcggaa tcggcgccgc catgtcgtgc
     1561 tacgacaccg cccgccagta ttcgctcgtc cgcaaacagt tccgcgacca gcccatcgcc
     1621 agtcatcagc tcgttcagga aaagcttgca tggatgatca ccgaaatcac caaaggccag
     1681 ctcctcgtac tccaggtggg ccgcctcaag gaccagggca aagtcgagca ccagcacatc
     1741 tccatggcca aacgcaacaa catctggatg gcgctcgagt gcgcgcgcat ggcccgggac
     1801 atcctcggcg ccaacggcat caccgacgac ttcccggtga tgcgccacat gatgaatctg
     1861 gagtcggtca agacctacga gggcacgcat gacatccaca ccctcatcct gggccagagc
     1921 gtgacgggca tcgctgccta ttagcttggc gaggcccact ctcaactcgc aactcacaac
     1981 cacaactcac aactcacaac tcacaactct caactcacaa ctctcaactc tcaactcaca
     2041 actcacaact cacaatcgat accggcacat gaaactactc tccatccagt gtggattacc
     2101 gcgggaagtc acctggcatg gaggcaggtt caccacctct attttcaagg agccggtcct
     2161 gggccgcatc gccctgcgca cgctcaatct cgatggcgac cgtcagtccg acctcaccgt
     2221 ccacggcggc aagtacaagg ctgtctattg ctatccggtg cagcactacg agtactggag
     2281 aagggaacta cccgggcgcg acctgccctg gggcgctttc ggcgagaatt tcaccatcga
     2341 agacggtccg gatgagccat cggtccacat cggtgaccgg ttctccattg ggtcttcgga
     2401 agtcgtagtc acgcagcccc gctttccctg ctacaagctc ggcatcaaat tccaatccga
     2461 cgacatggtc aggcgctttc tggccagcgg gcgctccggc ttctacctgg ccgttacgcg
     2521 agaaggggaa gtgggcgccg gcgacgaaat caccccgctc agccacgatc cggacggggt
     2581 ctccattgcg gacatcattc gcctctatat tgcgaaggag ttcagcgaca ccgacgttcg
     2641 ccagcttcaa aaagccctgg ttctggaggc tttccccgac agctggaagg actatcttaa
     2701 ggaaaagatg gagcgtctgg ccgggtgagc ccccgccggc ctctctttcg gaggcccccg
     2761 caaccccccc acgccgcccg cgtctaacca tgcagcccgg ctggcccggg tagacccaca
     2821 tgcatttccc caagcttcag cgccttcccg aactcgacgc cctccgcggc ctgttcctcg
     2881 tctggatgac gctgacccat cttcccaccc acgtcagcac gttcctgaat gagccctttg
     2941 gcttcgtctc ttccgccgaa gggttcgtct tcctctccgc gatcctgatc ggccggctct
     3001 atatccgccg gtcgatggaa gacccggtcg gcgtgcgctc ccacatgtgg cgtcgctcct
     3061 tcaagcttta cgcctaccac ctgttcctgc tcctgttcgc ctttaccctc gccgccagct
     3121 gggcggtcca tcaccaccgc gtggccatca ccaacctgct caacttctat ctgaaccatc
     3181 ccggcgtcgc gatcactggc tccatcttcc tcatttattg cccccctctg ctcgacatcc
     3241 tgcccctgta cgtgatgttc ctcttcctca ccccagcctt tctctcggcc gcgatccgct
     3301 ggggctggaa gaccgtcctc agcgtcagcg cgctcatctg gctgggcgct cagttcggcc
     3361 tgcgcgacgt ggtgcacagc gccgtcgtcc atctcaccca cctgcaaatc cccctccagg
     3421 aaaccggtgc cttcaacctc ttcgcctggc aaatggtctg gatcgtcggc ctctggcttg
     3481 gcgcctgctc cgcgcatcgc gatgtcccac tgaagctggt gccgcgttgg ggagtcgcgc
     3541 tcagtgccgc tgtttgcctc tttttcgtcg gcgtgcgctt cagctggctg ggcccccacc
     3601 tcaccgagca gtctctcggc ctcctgctcg acaagtggca gatcggtccg cttcgcgtcc
     3661 tcaacatcgc agccttcctc gtcgtcttct actggctgcg ccggtatatc cgcgtcgtgg
     3721 tcgcgcgcga gcccttcctc accttcggca aggcctcgct ccaggtcttc tgcgcgcatc
     3781 tcctcttcgt cttcgggggt cttgccctgc tggtcggcga aatggagcag ctcaccggct
     3841 ggaccgccat cgcgctcctt gctgtcacct tcgtctctct tttcctcgtg gccgccaacg
     3901 aagtccgcaa gaagcgtcgc gccaaacagg ggaacccgaa ccctcccaac cccggtgtgc
     3961 cctcacttcc gccacccgac cggcccgtca acacgcagcc actcgctgcc gtcgccctcg
     4021 aagaacgccc tctctgagcg tttcgatggc ttcaggatct cgtaaacatc gcaaaccggt
     4081 cccggatcga agcacgtgat ggcgccgcaa agcggcgcga cggtagaagc cacggccttc
     4141 aggccgtgga atcgcacgca tcgaaagaag aggggcttta gccccggcct ctttgctaac
     4201 ggactaaact acccgaggaa cgc
//
LOCUS       DASZYN010000149        10175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_197663, whole genome shotgun sequence.
ACCESSION   DASZYN010000149 DASZYN010000000
VERSION     DASZYN010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 10175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10175
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197663"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VHE33_03890"
     CDS             <1..394
                     /locus_tag="VHE33_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96622.1"
                     /translation="DKSVIQEYLDFRHVHHVPVWLGESGENKDAWVQGFRQTLEANNV
                     GWAFWTYKRMDDSPCVVTFDRPVHWDEIVAYAKLPRATADAEKNLAARPPQEDIDAAF
                     ADLLKKIAFGSERVNAGYLRALGMESSQ"
     gene            675..3248
                     /locus_tag="VHE33_03895"
     CDS             675..3248
                     /locus_tag="VHE33_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT96623.1"
                     /translation="MFSDLRQALRQMRKSPGFTVTAVVTLGLGIGATTAIFTLIQQVM
                     LRSLPVQKPGELWKIGDKNRCCNWGGYTETSSDAPNEWSLFPWELYNQFRANTPAFTE
                     LAAFQAGNTQLGVRAAGSPEQAQTRNGEFVSGNFFQTFGVGAWMGRLLTPADDQEGAP
                     PVAVISYRSWLQHYGADRGVVGRSYLIDGHPFTVVGIAAPSFYGAILAGWNMPDFWMP
                     LTSEPLVDGTTSRLKSPSEKFLDLIGRVKPGTNPKVLEAQLRAELHAWQASHVPDMSP
                     QEKQTWQQNTLHLVPGGAGVDTMADSYGDGLKLLMAAAGCVLLVACANLANLLLARGL
                     KNRQQTSIRVALGASRGRLVRKALVESVSLGVLGGAAAIAVAYGGAKLVVHLAFQIGG
                     PGNWVPIDVSPSWMVLGFALGISVVTGMLFGIAPAWMTSHAEPVEALRGARGSTGTKA
                     RWPQKALVVAQAAMSLVLLSAAAMLAQSLRNLQHQDFGFAVKDRYLVWTNTQLTGYPA
                     DQMPRLFRRIDERLAAIPGVRSAAAATYAPMSGDNWSMGIRVEGQPEPGAKDNTSALY
                     SRVTPGFFETLGGRMVAGRPITNDDSAAAQRVAVVNETFAKKFFQGKSAIGQHFGPAA
                     GKYAGLYTVVGVVGDMRFTAWNFDRPIEPMYYLPEAQTAHFDEAQMNSGEAWSHYLSN
                     IALWAPGHPADLQAQVKRALAEIDPNLVMQGVDKYSDVLDGDFQQQNMIATLALLFGA
                     VGLVLAAVGLYGVTAYTVEQRTSEIGVRMALGADRRSVVKMVLRGAFLQVAVGLALGI
                     PAAILAGHAIADQLFGVKAWDPAMLGGATVVLGMAALVAAIIPAQRAANLDPVESLRA
                     E"
     gene            3373..5811
                     /locus_tag="VHE33_03900"
     CDS             3373..5811
                     /locus_tag="VHE33_03900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT96624.1"
                     /translation="MLNDLRFAWRQLKRAPGFALTAVLTLALGIGATTAIFTLVYDVM
                     LRPLPLPQPEQLVRVQEKVAEWRDMYPTLPVSANHFTFWQQHSKSFAAIAALEEYAMP
                     FESGEAQGGRAMHVDVVSATPGIFTVLGVQPALGRAFNESEGQKGHERVAVITDALWR
                     EQFGGDREILGRTLKLDGFTYTVVGVMPASFRMPSTWGGKEPLSAIVPMAFDKELLEE
                     AMGDLNYFGLARLKPGVTVAAANAELNAEQKAIASTLPANQKATLSAVLTPWQEDLVG
                     GSRKALMILLGAVAGLLLVGCVNIANLLLARAAGQKHQMAVAAALGARRSEMLRMAMR
                     ETVVLAALGCGLGIVLAMAIVPLMQHYLPPELNFRGALHLDWVGAGCALLLAGAATLL
                     AGAAPAWMVSRTAPQEVLHSESRLASESRGSKHARRVLVGVEVAVSVALVLMTGLLVA
                     SLTKLMRVDRGFDTERTLAVKVELPYEAYPNRPKRAEFYRDALVKLNALPGVEQAALT
                     SVLPLSGNGWGDIAQLPGDTRPWTQLPGEEWRWVSPNYFETIHLRLIRGRFFNQSEWG
                     KNEAVLSERTAKALWGDKDPVGQQFHRAGAEGEAPFTVVGVVSNARTVTLAKADPMII
                     YVPYWFRADADGGIVMRTRQAPAAMAESIRKAIWSVDSGVAVPQIRLLSGVVADSVAD
                     RRFEMDLLVVFAVSALLLAGLGVYGVVTYSVVQRQREIGLRIALGAQRANIYRLILRE
                     GLLPVVAGAGAGIALALGSARLVGSLLFEVSPYDPGIAAGAVSLLLVAGMSACWLPAR
                     RAAQVEPMEALRAE"
     gene            6006..8471
                     /locus_tag="VHE33_03905"
     CDS             6006..8471
                     /locus_tag="VHE33_03905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT96625.1"
                     /translation="MWSDLRFAVRQLRKSPGFSLTAVLTLALGIGANTAIFTLVDSIM
                     LRPLPYPQQERLMRINYGGGEAVAAYFPKGWIRALGENSKAFEGISGFGPDAESNVGY
                     ANASNRVFGAEVMVNAFDLLQVHPVLGRFFTAEDARGGHDPVVVLSYGYWRQHFGGSP
                     GVLSTTIRIDGISRRVIGVMPAGVRFPYADTQFVTPVTFKSGDVFDPWSNFDLRAFGR
                     LRPGVTPAQAQSEMHPLQKHLLNSFPFRMPDIWASDMRVVPLLESEVGAMRPRLLLLF
                     GAVGLILLIACANVANLMLARAAGREKEIAVRSALGATGSRLVRQLLAESVVLGVVAG
                     LLGLLAAAASLQALISLLPADTPRLADVSLHWPVFLFAALASVVTGVLFGLIPAVRMA
                     SPNLRESLHSGSRTVAGKAGQFRVSMLLVMGQIALSVVVITAAGLMLHSLWSLAQVDP
                     GFRTDRIVTAEVSLDKTMCPGLLGSPENGQGRCQQFFATLLDRLQGLPGLESASMTNL
                     LPLSGQDGNYVYDAEGHPRTPREGARLAAGRVVSPDYFATLGIQLVRGRLLEEQDLSG
                     TSRALVIDQKMAEELWPNQNPLGKHLIDVAAEKTPGAIDMNAASVVVGVVTNTHERTV
                     ASNFGDEVYAPMTKQLESPVMYVLLRTRATTEEAASELRQAVAAINPEVPVTRLRTLD
                     EVVASSQSASRSLTILLLAFGGLAVVIGGVGVYSLIAYIVSWRTREIGIRLALGAQRW
                     QIVQGVMRQSLVLALGGSVAGLVVAALVGQLLRSFLFGVKPVDPVTFVGVAVLMMALA
                     LTAAWVPARRAASVDPMKTLRSE"
     gene            8571..>10175
                     /locus_tag="VHE33_03910"
     CDS             8571..>10175
                     /locus_tag="VHE33_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT96626.1"
                     /translation="MLNDLKYAWRQILKAPGFAITALLTLAIGIGVNAAVFSVMDAIV
                     LRPLAVPNLDRVMTVAEDRGRGRFSYEWVTGANYEDWRQQSKSFESLAAFKRYSMALT
                     GAGEAAHVSASRSTANFWNVLKIDAFMGRVFRADECQPGRDAVVLLSYSFWQKQFASD
                     PNVLGRKIRLDERDYEVIGVMPKGLVYPPSTDLYLPLALTPAQLHDRGDHEYMVVGRL
                     RPGVTAREADSELHGIAARLQKEYPATNLGWSVKVQALLDNISGELTPLYMRLMMGGT
                     LFVLLIVCANVANLQFARGVARRPEMAMRTALGAPRNLLLRQLLTENVVLSVAGAAAG
                     MLLAWLDLHLCLIAMPERVARYVAGWYTIHLNMRALIFSALLAVAAGVVSGLAPALEA
                     MRVNLVEQLKSGSRTETGGSKNHRLRNFFATAQIALAVALVIGAALMCKGMWSTLQFG
                     NVYHPKQVLTFGVTLPAVRYGDDVKKQAWYQSSLEKIQSLPGVKRAAVTTMLPYGQGG
                     WTDDFRIENRPVTPGKFDSAVRVAMSQ"
BASE COUNT         1713 a         2532 c         4043 g         1887 t
ORIGIN      
        1 ggacaagagc gtgatccagg agtatctgga cttccggcat gtgcatcatg ttccggtgtg
       61 gctgggcgag agtggcgaga acaaggatgc gtgggtgcag gggttccgcc agacactgga
      121 ggcgaacaac gttggctggg ccttctggac gtacaagcgg atggacgact cgccgtgtgt
      181 ggtgacgttc gaccggccgg tgcactggga tgagattgtg gcctacgcca aactgccgag
      241 ggcaacggcg gatgcggaga agaacctggc cgctcggcct cctcaggagg atatcgacgc
      301 agcgttcgcg gacctgctga agaagattgc cttcggcagc gagcgggtga atgcggggta
      361 tctgagagcg ctggggatgg agagcagcca atagccggta gccgtcagga atggcaaaaa
      421 acgagcagcg aacggactgg atcgcgctgc gcgatgccca tctcgcgacc gcagggacat
      481 ggatacccgg ctttttcggt tgcgaatccc tgttcggttc tgaacagggc gggatgaatc
      541 tggacaatcg atgggccgtc gatgggcggt gaaccctttt gtttcaatgg cgtagggatg
      601 ggtgcgtctg gtggaacgca tgcttttctc ttcctcaacc gtgcgttgag ttttgggcgg
      661 gagcggagga gaacttgttt tctgatctga gacaggcttt gcggcagatg cggaagtcgc
      721 cggggtttac cgtgacggcg gtcgtgaccc tggggttggg gatcggcgcg accacggcga
      781 tcttcacgct gatccagcag gtgatgctgc gctcgctgcc ggtgcagaag ccgggcgagt
      841 tgtggaagat cggcgacaag aatcgctgct gcaactgggg cggctacacg gagaccagca
      901 gcgacgcgcc caatgagtgg agcctgttcc cctgggagct ctacaaccag ttccgcgcga
      961 atacgccggc gttcacagag cttgcggcgt tccaggcagg caacacccag ctgggcgtgc
     1021 gcgcggcggg cagcccggag caggcccaga cgcggaacgg cgagtttgtc tccggaaatt
     1081 tctttcagac cttcggcgtt ggggcatgga tgggacggct gctgacgccg gccgatgatc
     1141 aggaaggcgc gccgccggtg gcggtgatca gttaccgcag ctggctgcag cattacggtg
     1201 cggatcgcgg ggtcgtgggg cggagttacc tgatcgatgg gcatccgttc accgtggtgg
     1261 gcatcgcggc gccgtcgttt tacggagcga ttctggcggg ctggaacatg ccggacttct
     1321 ggatgccgct gacgagcgag cccctggtgg atggaacgac atcgcggctg aagagtccga
     1381 gtgagaagtt cctggatttg attgggcgag tgaagccggg aaccaatccg aaggtgctgg
     1441 aggcgcaact gcgggcggag ctgcatgcgt ggcaggcgag ccatgtgccg gatatgagtc
     1501 cgcaggaaaa acagacctgg cagcagaaca cgctgcacct ggtgcccggc ggtgcgggcg
     1561 tggacacgat ggcggacagc tacggcgatg ggctgaagct gctgatggcg gcggcagggt
     1621 gtgtgttgct ggtggcgtgt gcgaacctgg ccaatctgct gctggcgcgg ggactgaaga
     1681 atcgccagca aacgtcgatc cgcgtggcgc tgggcgcatc gcgcgggcga ctcgtgcgta
     1741 aggcgctggt ggagagtgtg agcctggggg tgctgggcgg tgcggctgcc atcgccgtgg
     1801 cttatggcgg cgcaaaactt gttgtccacc tggcgtttca gattggcggt ccggggaact
     1861 gggtgccgat cgatgtgtcg ccgtcgtgga tggttttggg atttgcgctg ggcatttcgg
     1921 tcgtgacagg aatgttgttt gggatcgcgc ccgcgtggat gacctcgcac gcggagccgg
     1981 tggaggcgct gcgtggggcg cggggttcga ccggaacgaa ggcgcggtgg ccgcagaagg
     2041 cgctggtggt ggcgcaggct gcgatgtcgc tggtgctgct gagcgcggcg gcgatgctgg
     2101 ctcagagcct gcgcaacctg cagcatcagg actttggctt tgctgtgaaa gaccggtatc
     2161 tggtgtggac gaacacacag ctgaccgggt acccagcgga ccagatgccg cgcctgtttc
     2221 gacggattga cgaacggctg gcggcgattc ccggagtgcg gagcgcggcg gcggcaactt
     2281 acgctccgat gagcggggac aactggagca tgggcattcg ggtagaggga cagccggagc
     2341 cgggtgccaa ggacaatacc agcgcgctct attcgcgggt gacgccaggg tttttcgaaa
     2401 cgctgggggg ccgcatggtg gcggggcggc caattacgaa cgacgactca gcggcggcgc
     2461 agcgggttgc ggtcgtcaac gagacctttg caaagaagtt ctttcaaggc aagagtgcga
     2521 tcggacaaca tttcgggccg gcggcgggga agtatgcggg gctgtacacg gtggtgggcg
     2581 tggtaggcga tatgcgattt acggcgtgga atttcgaccg ccccatcgag ccgatgtact
     2641 acctgcccga agcgcagacg gcgcacttcg acgaagcgca gatgaacagc ggcgaggcgt
     2701 ggtcgcacta cctgtccaac attgcgctgt gggcgccggg gcacccggcg gatctgcagg
     2761 cgcaggtgaa gagggcgctg gcggagatcg atccgaacct ggtgatgcag ggcgtggaca
     2821 agtactccga tgtgctggac ggagacttcc agcagcagaa catgattgcg acgctggcgt
     2881 tgctgttcgg cgcggtgggt ctggtgctgg ctgccgttgg cttgtatggc gtgacggcct
     2941 acacggtgga gcagcggaca agcgagatcg gggtgcgcat ggcgctgggc gcggatcggc
     3001 ggagcgtggt gaagatggtg ctgcgaggcg cattcctgca ggtggcggtc gggttggcgc
     3061 tggggattcc tgcggcgatt ctggccggac atgcgattgc agaccagctg tttggcgtga
     3121 aggcgtggga tccggcgatg ctgggcggag cgacggtggt attgggcatg gcagcgctgg
     3181 tagcagccat cattccggca cagcgggccg cgaatctgga tccggtggag tcgttgcggg
     3241 cggagtaagg cgcgtgagcg cttcactccg cagctaagaa gcggtcagct aaggggcggt
     3301 cagctaaaaa gcggtcagct gaaaaccggt tagcggaagg cggacgggca agagcagcag
     3361 cggaggcgag ggatgctgaa cgatctgcga ttcgcgtgga ggcagttgaa gagggcgccg
     3421 gggtttgcgc tgacggcggt gctgacgctg gcgctgggga ttggggcgac gacggcgatc
     3481 ttcacgctgg tgtatgacgt gatgctgcgg ccgttgcctt tgccgcagcc ggagcaactg
     3541 gtgcgtgtgc aggagaaggt tgcggagtgg cgcgacatgt atccgacgct gccggtgagc
     3601 gcgaatcact tcacgttctg gcagcagcac agcaagagct ttgcggcgat cgcggcgctg
     3661 gaggagtatg ccatgccgtt cgagagcggc gaggcgcagg gcggacgcgc gatgcacgtg
     3721 gatgtggtga gcgcgacgcc gggaatcttc accgtgctgg gcgtgcagcc ggcgctggga
     3781 cgcgcgttca atgagagcga agggcagaag ggccatgagc gcgtggcggt gatcaccgat
     3841 gcgctgtggc gggagcagtt cgggggcgac cgggaaatcc tcggacggac tctgaagctg
     3901 gacggcttca cgtacacggt ggtgggagtg atgccggcgt cgttccggat gccgtcgacg
     3961 tggggcggca aagagccgtt gagcgcgatt gtgccgatgg cgttcgataa ggaactgctg
     4021 gaagaagcga tgggggacct gaactacttt gggctggcgc ggctgaagcc tggagtgacg
     4081 gtcgcagcgg cgaatgcgga gctgaatgcc gagcagaagg cgattgcctc cacgctgccc
     4141 gcgaatcaga aggcgacgct gtccgcagtg ctgacgccat ggcaggaaga tttggtgggc
     4201 gggagccgga aggcgctgat gatcctgctg ggcgcggtgg cgggactgct gctggtgggg
     4261 tgcgtcaaca ttgcgaattt gctgctggcg cgcgcggctg ggcagaagca tcagatggcg
     4321 gttgcggcgg cgctgggcgc gcggcggagc gagatgctgc gcatggccat gcgcgagacg
     4381 gtggtgctgg ctgcgttggg gtgcggtttg gggatcgtgc tggcgatggc cattgtgccg
     4441 ttgatgcagc actatctgcc gccggagctg aatttccggg gtgcgctgca tctggactgg
     4501 gttggcgccg gatgcgcgct gctgctggcg ggggcagcca cgctgctcgc cggggcggcg
     4561 ccagcgtgga tggtgtcccg cacggctcca caggaggtgt tgcacagcga gtcgcggctg
     4621 gccagtgagt cgcgtgggag caagcacgcc aggcgggtgc tggtgggcgt cgaggtggcg
     4681 gtgagcgtag cgctggtgct gatgacgggg ttgctggtgg caagcctgac gaagctgatg
     4741 cgcgtggacc gcggattcga tacagaacgc acgctggcgg tgaaggtgga gttgccgtat
     4801 gaggcgtacc ccaaccggcc gaagcgcgcg gagttctaca gggatgcgct ggtgaagctg
     4861 aacgcgctgc cgggcgtgga gcaggcggcg ctgacgtcgg ttctgccgct cagcggcaac
     4921 ggctggggcg acatagcgca actgccggga gatacccggc cgtggacgca gttgccggga
     4981 gaagagtggc ggtgggtcag ccccaattat ttcgaaacga tccatctgcg gctgatccga
     5041 gggcgcttct ttaaccagag cgaatgggga aagaacgagg cggtgctgtc ggagcgcacg
     5101 gcgaaggccc tgtgggggga taaggatccg gtagggcagc agttccatcg tgcgggagcc
     5161 gagggcgagg cgccgttcac ggtggtgggc gtggtgagca atgcgcggac agtgaccctg
     5221 gcgaaagccg atccgatgat catctacgta ccgtactggt tccgcgcgga cgccgatggc
     5281 ggcattgtga tgcggacgcg acaggctccg gcggcgatgg cggagtcgat tcgaaaagcg
     5341 atctggagcg tggatagcgg cgtcgcagtg ccgcagatcc ggctgctgag cggggtggtg
     5401 gccgattcag tagcggatcg acgctttgag atggatctgc tggtggtctt cgcagtgagc
     5461 gcattgctgc tggccggact gggcgtgtat ggcgtggtga cgtattcggt agtgcagcgg
     5521 cagcgggaga tcgggctgcg cattgctctg ggtgcgcagc gggcgaatat ctatcggctg
     5581 attttgcggg aagggctgct gccggtggtg gcgggcgcag gagcggggat cgcgctggct
     5641 ctgggatcgg cacggcttgt cgggagtttg ctcttcgagg tgagccccta cgatccgggg
     5701 atcgcggcgg gggcggtgag tttattgctg gtggccggaa tgtcagcctg ttggctgccg
     5761 gcgcggaggg ccgcgcaggt ggagccgatg gaggcgttgc gggcggagta aggcgtgctt
     5821 tgcgcgcctt actccgcagc taagaggcgg ttagctaagg tccggttagc taagaagtgg
     5881 ttagctaaaa aggggtcagg aaacggggtc agcggaaaag cggttcgcta agcgcagaac
     5941 gaacgacgag cgagagtaac gggaacgaaa cggcagcggc gaagaccaat ctgaggtgat
     6001 gagccatgtg gagtgatttg agatttgcgg tgcggcaatt gcggaagtcc ccggggtttt
     6061 cgctgacggc ggttctgacg ctggcgctgg gaattggcgc gaacacggcg atctttacgc
     6121 tggtggattc gatcatgctg cggccgctgc cttatccgca gcaggagcgg ttgatgcgga
     6181 tcaactatgg cgggggcgag gcggtcgcgg cgtattttcc caagggctgg atccgcgccc
     6241 tgggcgagaa ctcgaaggcg ttcgaaggga tcagcgggtt tgggccggat gcggagtcga
     6301 acgtgggata cgcgaacgcg tcgaaccgcg tgtttggcgc cgaagtgatg gtgaacgcgt
     6361 tcgacctgct ccaggttcat ccggttctgg ggcggttctt caccgcagaa gatgcgcgcg
     6421 gcggacatga tccggtggtg gtgctgagct acggctactg gcggcagcac tttggcggga
     6481 gtccgggcgt gctgagcacg acgatccgca tcgatggaat ttcacggagg gtgattgggg
     6541 tgatgccggc gggcgtgcgt tttccgtacg cggatacgca gtttgtgacg ccggtgacgt
     6601 ttaagagcgg ggatgtattc gatccgtggt cgaacttcga tctgcgggcg tttgggcggc
     6661 tgcggcccgg tgtcacgccg gcgcaggcgc agagcgaaat gcatccgctg cagaagcact
     6721 tgctgaattc attccctttc cggatgccgg atatctgggc atcggacatg agggtggtgc
     6781 cgctgctgga gtcggaagtg ggggcgatgc ggccgaggct gctgctgctg tttggagcgg
     6841 tggggctgat cctgctgatt gcgtgtgcga acgtcgcgaa cctgatgctg gcgcgcgcgg
     6901 cggggcggga gaaggaaatt gcggtgcgca gcgcgctggg ggcgacgggg tcgcggctgg
     6961 tgcgacagtt gctggcggaa agcgtggtgc tgggcgtggt ggcgggcctg ctgggactgc
     7021 tggctgcggc ggccagcctg caggcgctga tttcgctgct gcccgcggat acaccgcgac
     7081 tggcggacgt ctcgctgcac tggccggtgt ttctgtttgc agcgctggcg tcggtggtga
     7141 cgggcgtttt gttcgggctg attccggcgg tgcggatggc gtcgccgaac ctgcgcgaaa
     7201 gcctgcactc gggcagccgc acggtggcgg ggaaggcggg ccagttccgc gtatcgatgc
     7261 tgctggtgat ggggcagatc gccctgtcgg tagtggtgat tacggcggcg gggctgatgc
     7321 tgcacagcct gtggagcctg gcgcaggtgg atcctggatt ccggacggac cggatcgtca
     7381 cggcggaggt ttcgctggac aagacgatgt gtccggggct gctgggatcg ccggagaatg
     7441 ggcaggggcg atgccagcaa tttttcgcga cgcttttgga tcggctgcag gggctgccgg
     7501 ggctggagag cgcgtcgatg acgaatctgt tgccgctgag cggacaggac ggcaactatg
     7561 tgtacgacgc ggaggggcac ccgcggacgc cgcgcgaagg agcgaggctg gcggcgggac
     7621 gcgtggtgtc gccggactac tttgcgacgc tgggcatcca gctggtgcgc gggcgtctgc
     7681 tggaggagca ggacctgtcg ggaacgagcc gggcgctggt gatcgaccag aagatggcgg
     7741 aggagttgtg gccgaatcag aacccgctgg gaaagcacct gatcgatgtc gcggcagaga
     7801 agacaccggg tgcgatcgat atgaatgcgg cgtcggtggt ggtgggcgtc gtgaccaaca
     7861 cgcacgaacg cacggtggcg agcaactttg gcgatgaagt gtacgcgccg atgacgaagc
     7921 aactggagtc gccggtgatg tatgtgctgc tgcgcacgcg ggcgacgacg gaggaagcgg
     7981 ccagcgagct gcggcaggcg gtggcggcga tcaatccaga ggtgccggta acgcgactgc
     8041 ggacgctcga tgaggtggtg gcatcgtcgc agtcggcctc gcgttcgctg acgattctgc
     8101 tgctggcgtt tggcgggctg gcggtggtga tcgggggcgt gggtgtctac agcctcattg
     8161 cctatatcgt cagctggcgg acgcgggaga tcgggatcag actggctttg ggagcgcagc
     8221 gctggcagat cgtgcagggc gtgatgcggc agagcctggt gctggcgctg ggcggatcgg
     8281 tggcggggct ggtggtggcg gctttggtgg ggcaactgct gcggagcttc ctgtttgggg
     8341 tgaagccagt cgatccggtg acgttcgtgg gtgtggccgt gctgatgatg gcgctggcgc
     8401 tgaccgcggc gtgggttccg gcgagaaggg cagcgagtgt ggatccgatg aagacgttaa
     8461 ggagtgagta agccgtaggc ttactcgctc cagctaagaa gcggtcggct aagagccgat
     8521 tagcgaactg ctagccgagc agaagcgtat gagtccgtga ggggcctgca atgttgaacg
     8581 atttgaagta tgcgtggagg caaattctaa aggcgccggg attcgccatt acggcgctgt
     8641 tgacgctggc gattgggatt ggtgtcaacg cggcggtgtt cagcgtgatg gacgcgatcg
     8701 tgctgcggcc cctggcggtg ccgaacctgg accgcgtgat gacggtcgcc gaggatcgcg
     8761 ggcggggtcg gttctcgtat gagtgggtga cgggggccaa ctacgaggac tggcggcagc
     8821 agagcaagtc tttcgagagc ctcgcggcgt tcaagcgata ctcgatggcg ctgaccggag
     8881 caggggaggc ggcgcatgtg tcggcgtcgc gctcgacggc gaatttctgg aacgtgctga
     8941 agatcgatgc gttcatggga cgggtctttc gcgcggatga gtgccagccg gggcgtgacg
     9001 cggttgtatt gctcagctac agcttctggc aaaagcagtt cgcatcggat ccgaatgtgc
     9061 tggggcggaa gatccggctg gatgagcgag actacgaagt catcggcgtg atgccgaagg
     9121 ggctggtgta cccaccctcg acggatttgt atctgcccct ggcactgact ccggcgcaac
     9181 tgcacgatcg cggcgaccac gaatatatgg tggtgggacg tctgcggccg ggtgtgacgg
     9241 cgcgtgaagc cgattccgaa ttgcacggga tcgcggcgcg actgcagaag gagtatccgg
     9301 cgacgaatct gggatggtcg gtgaaggtgc aggcgctgct ggacaacatc agcggggagc
     9361 tgacgcctct ttatatgcgg ctgatgatgg gcgggacgct gttcgtgctg ctgatcgtgt
     9421 gcgcgaacgt ggccaatctg cagtttgcgc gcggtgtggc gcggcggccg gagatggcga
     9481 tgcgcacggc tttgggtgcg ccgcggaatc tgctgctgcg gcagttgctg acggaaaacg
     9541 tggtgctgtc ggtagccggg gcagccgccg ggatgctgct ggcgtggctg gatctgcatc
     9601 tgtgtttgat cgccatgccg gagcgggtgg cacgctatgt ggcgggctgg tacacgattc
     9661 atctgaatat gcgggcgctg attttctcgg cgctgctcgc ggtggcggcg ggtgtcgtct
     9721 ccggcctggc gccggcgctg gaagcgatgc gggtgaatct ggtggagcag ctgaagtcgg
     9781 gctcgcgcac cgagaccggc gggagcaaga accatcggct gcggaacttc ttcgcgacgg
     9841 cgcagattgc gctggcggtg gcgctggtga ttggggcggc gctgatgtgc aagggcatgt
     9901 ggtcgacgct gcagtttggg aatgtatatc accccaagca ggtgctgacc tttggcgtga
     9961 cgctgccggc ggtgcgctat ggagacgatg ttaaaaagca ggcgtggtac cagtcgagcc
    10021 tcgagaagat ccagagcctg ccgggtgtga agcgcgcggc ggtgacgacc atgcttccct
    10081 acggccaggg cggctggacg gatgatttcc ggattgagaa tcggccggtg acgccgggca
    10141 agttcgacag cgcggtgcgc gtggcgatga gccag
//
LOCUS       DASZYN010000150         3710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_388488, whole genome shotgun sequence.
ACCESSION   DASZYN010000150 DASZYN010000000
VERSION     DASZYN010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388488"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /gene="dnaJ"
                     /locus_tag="VHE33_03915"
     CDS             complement(<1..950)
                     /gene="dnaJ"
                     /locus_tag="VHE33_03915"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HVT96627.1"
                     /translation="MSRTENVIKADYYEVLGVARSASDQELKAAYRKLALQHHPDRNP
                     GNKESEERFKECSEAYQVLSDPQKRAAYDRFGHAGVGGGGFDGNPFGGQDIGDIFGDL
                     FGEMFNMGGNRRGQSRVQKGRDIRYDLTIEFEEAVFGKETKVTIQRMEACADCRGSGT
                     ASGRGPATCPHCQGRGQVRYQQGFFSIARTCSYCSGSGTVISDPCTTCRGEGRVERQH
                     EIAVSIPAGVEDGTRIRYQGEGDTGRFGGPGGDLYIVLRVRPHKVFERDGNDLHCLIP
                     ISFPQAALGAEINLDTLDGAVKLKIPEGAQSGQEFRIRGKG"
     gene            complement(947..1567)
                     /locus_tag="VHE33_03920"
     CDS             complement(947..1567)
                     /locus_tag="VHE33_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HVT96628.1"
                     /translation="MRRIMVEKKNTAENMEGAPAEDATNGEGAVAAGFADNEETKEPL
                     VPVETGHVPQEEYDRLKAERDLLFDRLARLQAEFDNARKREAKERTEFRDYAVSNAVE
                     QFLPVLDNFQLALKSTGSAEQLRTGVELIVKQMEEALRSLNIQPVEAVGATFDPRVHE
                     ALDMVERSDVPDHQVFEEVRRGYRMRERLLRPALVRVASNSQQKEA"
     gene            complement(1646..2707)
                     /gene="hrcA"
                     /locus_tag="VHE33_03925"
     CDS             complement(1646..2707)
                     /gene="hrcA"
                     /locus_tag="VHE33_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HVT96629.1"
                     /translation="MASELRLSPRERSVLTAIVETYIATGEPVASQAVARLFGNKDGM
                     SSATIRNVMVSLGDAGLLDQPHTSAGRIPTAQAFRFYVEHLAGPQVTILAPERMEQIE
                     ENFAGIGTSEQYLERTSHVLALLSSGLGVALASTQELHTLEHIHFSRLAAGRVLGVVI
                     TKAGSVLDRVLLLDHDLNAGELESAARYLNENFYGWSIERIRIEMARRLEAERSEYDR
                     LMHSLEELYRKGALAAETSLAPMIFMEGVANLLSGETDRERLRTMLHALEAKERLIEL
                     LNAYVDARQRTVRVVVGLEDAIPELRDFVLIGTTARLGTENVGTVAVIGPTRIHYQET
                     INAVSYIAQLSDRILQPPH"
BASE COUNT          705 a         1360 c          924 g          721 t
ORIGIN      
        1 cccttgccgc ggatgcggaa ctcctgcccg ctctgcgctc cctcgggaat cttcagcttc
       61 accgcgccat cgagcgtatc cagattgatc tccgcgccca gggccgcctg cggaaacgaa
      121 atcggaatca ggcagtgcag atcgttgccg tcccgttcga ataccttgtg cggccgaacc
      181 cgcagcacga tataaagatc gccccccggc ccgccgaacc gccccgtgtc cccttcgccc
      241 tggtaccgga tccgcgtccc atcctcaact ccggcaggaa tgctgactgc gatttcgtgc
      301 tgtcgctcca cgcgtccttc gccccggcag gtcgtgcacg gatcgctgat caccgtcccc
      361 gagccgctgc aataactgca cgtgcgcgca atcgagaaaa atccctgctg ataacgaacc
      421 tggccgcgcc cctggcaatg gggacaggtt gccggcccgc gcccgctcgc cgtgcccgac
      481 ccgcggcaat ccgcgcatgc ctccatgcgc tggatcgtga ccttcgtctc cttcccaaag
      541 accgcctctt cgaactcgat cgtcaggtca taacgaatgt cgcggccctt ctgtacccgc
      601 gactgccccc gccggtttcc gcccatgttg aacatctcgc cgaacaaatc gccgaagatg
      661 tccccaatat cctgcccgcc gaacggattc ccgtcgaatc cccccccacc cactcccgca
      721 tgcccgaagc ggtcataggc cgcgcgcttc tgcgggtcgc tcagcacctg atacgcctcg
      781 ctgcattcct tgaagcgctc ctcggattcc ttgttccccg gattgcggtc gggatgatgc
      841 tgcagggcca gtttgcggta cgcggccttg agctcctgat cggaggcgct gcgagccacg
      901 ccaagaactt cgtaatagtc agctttgatc acgttttcgg ttctgctcat gcttccttct
      961 gctgtgagtt gctggccacc cgcaccaacg ccgggcgcag gagccgctcg cgcatacgat
     1021 agccgcgtcg cacttcctcg aatacctgat gatccggcac gtcactccgc tccaccatat
     1081 cgagcgcttc atgcacccgc ggatcgaacg tcgcccctac cgcctccacc ggctgaatgt
     1141 tcagcgaccg cagcgcctct tccatctgct tcacgatcag ctccaccccg gtgcgcagct
     1201 gttccgcgga tccggtcgac ttcagcgcca gctggaagtt atccagcacg ggcagaaact
     1261 gctccaccgc attcgagact gcatagtccc gaaactccgt ccgttccttc gcttcgcgct
     1321 tgcgcgcgtt gtcgaattcc gcctgcagcc gcgccagccg gtcgaagagc agatcccgct
     1381 ccgccttcag ccgatcgtac tcttcctgcg gcacgtgccc ggtctcgacg ggcaccagcg
     1441 gctctttcgt ttcctcgttg tcggcgaacc cggcggcgac agctccctcg ccattcgtgg
     1501 catcctcggc cggagcccct tccatgtttt cggcagtgtt cttcttctca accataatgc
     1561 gtcgcatgat tcaaactcga ttcggatacg cagccgtcga ttgctggcga ctgctaaggg
     1621 gaattccgcg cacgcgcgtg cgcgctcaat ggggcggctg cagaatcctg tcggaaagct
     1681 gtgcgatgta cgacaccgcg ttgatggttt cctggtaatg gatgcgcgtc gggccaatca
     1741 ccgccaccgt gccaacattc tccgtcccca gccgcgccgt agtgccgatc aggacaaagt
     1801 cgcggagctc gggaatcgcg tcctccaggc ccaccaccac ccgcaccgtc cgctgccgcg
     1861 cgtccacata cgcattcagc agctcgatca gccgctcctt ggcctccagc gcgtgcagca
     1921 tcgtgcgcag ccgttcgcga tccgtctctc cggacagcag gttggccacg ccttccataa
     1981 agatcatcgg cgccagactc gtctccgccg ccagcgctcc cttccggtac agctcctcca
     2041 gcgagtgcat caaccggtca tactcgctgc gttccgcttc cagacgccgc gccatttcga
     2101 tccgaatccg ctcgatcgac cagccataaa aattctcgtt caggtagcgc gcggcgctct
     2161 ccagctcgcc agcgttcaga tcgtggtcca gcagcagcac ccggtccagc acgctgcccg
     2221 ctttcgtgat caccaccccc agcacccgcc cggccgccag ccgcgaaaaa tggatgtgct
     2281 ccagcgtatg cagttcctgc gtcgaagcca gcgctactcc caaaccgctg gagagaagcg
     2341 ccagcacatg cgacgtgcgc tccagatact gctcgctcgt accgatcccc gcaaaatttt
     2401 cctcgatctg ctccatccgc tccggcgcca gaatcgttac ctgcggcccg gccaggtgct
     2461 ctacataaaa acgaaatgcc tgtgccgtcg gaatgcgtcc cgccgacgtg tgcggctgat
     2521 ccagcagccc ggcgtcgccc agcgacacca tcacgtttcg gatcgtcgcc gagctcatcc
     2581 cgtctttatt gccgaacagg cgagccactg cctgcgacgc aaccggttcc ccggtcgcga
     2641 tatacgtctc cacaatcgcc gtcagaaccg accgttcgcg aggactcaat cgcagttcag
     2701 atgccatagc aggatgcgca aaatcacccg caaaggggtg aatctctcct ctttagattg
     2761 taggtttcgc taaaacgccg gtcaagaaac ggaaattatt gcaatatcaa gagataacgt
     2821 ctgccatccc ggccgaagcg cgacccacct tgtcatcccg accgaagcga agcgaagcgg
     2881 agggactgct ttcctccacg acaccgcccc cacccctgtc atcccgaccg gagcggatgc
     2941 tcagcaacac gcgaagcgga gggacctgct tcccccaaca ccaccaccca cccacctaac
     3001 cttgtcatcc cgaccggagc gcgaggcgca gcaacgcgcg aagcggaggg acctgctttc
     3061 ccccaccctg tcatccccac aagcggaggg acctgctttt caagttagct gtccgcaacc
     3121 gccaattacc accgtaccgt cccacctcgc gaattccctt gcacctcaaa gtcccaccac
     3181 ctaccctgaa gatactggga gaaatatcta cccacgaaaa cgctcgccga tgaggcgagc
     3241 cttttccttt gcaaccgctc ggcccaggcc gatcttgtaa gtgctctttg acatcctcgc
     3301 agcggacggg acccacccaa agcccagtgc aatcccaagc cggctcgctc ccgcacaggg
     3361 ctcgccatgg gtcacattct cggcctcttc ggttacatac acggccgaga gtgttataca
     3421 aacggccgct tctgttacac aaacggccgt ttcgaagtat tccgctttca gcaagatagc
     3481 ctttgtcaaa cttgtaaaca atcgcaagca acgaagaatc aattacatga ctcaaggccc
     3541 tcaaaaactg tctcgcgaac ggcacctcac tgggccttta agctaaccgc tctttagcta
     3601 accggatctt agccgaccgg cctttagctg accggctctt agctccacac cgctccatca
     3661 gtactcgcct gtcaccacgt tctcgagcgg ctcgccccgc agataccgcc
//
LOCUS       DASZYN010000151         1996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_197727, whole genome shotgun sequence.
ACCESSION   DASZYN010000151 DASZYN010000000
VERSION     DASZYN010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197727"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VHE33_03930"
     CDS             complement(<1..200)
                     /locus_tag="VHE33_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96630.1"
                     /translation="MSTIVTLPDQSTAKLPKVSYLNKEYGLKSWLLTGDHKRIAMLYL
                     ISITFFFFIGGAMAGLIRMELLT"
     gene            complement(197..1276)
                     /gene="coxB"
                     /locus_tag="VHE33_03935"
     CDS             complement(197..1276)
                     /gene="coxB"
                     /locus_tag="VHE33_03935"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HVT96631.1"
                     /translation="MHFISPVLWQFLIKWLTYFAIFPPEASSKAAEMDALYFFLVSIS
                     VVGLGIVALLVVTFSIRYRRDLHPVATPIEGSTLLEATWTIIPLILFMVCFVWGALLY
                     YRIYSPPPNAMNIYVVGKQWMWKVEHPGGQHEINALHIPINKPVQITLISQDVFHSFS
                     IPAFRVKREAIPGRYNTEWFEATIPGTYHIFCTQYCGTLHSQMIGEVTAMTPEDYQAW
                     AAGSTSGQSLAQNGERLFASLGCNSCHNSSPNAHGPNLANIYGSRLQLKNGQFATVDD
                     AFLRDTILNPTLHEVAGYAPIMPTYQGQVSEEGLIDLVEYIRQLNSQYRIEQTIDNSA
                     AQPIQTGATPPGTSAPALPPGTVKP"
     gene            complement(1280..>1996)
                     /locus_tag="VHE33_03940"
     CDS             complement(1280..>1996)
                     /locus_tag="VHE33_03940"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HVT96632.1"
                     /translation="QLPLNLEFRDETGKAVHLGDYFGKRPVILSLVYYECKILCPEEI
                     DGLTGALEMLKFNPSRDYDVVLVSIDAKETPAIAAREKAFYMNRFGRPQDDNGWHFLT
                     GQEPAIRALATAVGFGYVPMQGPDGKMTQFAHASAIELVTPEGKLAQYYMGVEFSPKD
                     IQLGLVEASHGKIGSLVDNLLTYCYQYDPTANRHTLLYIRIFQLGWILTVLALGTFML
                     VNFRRDIRQAREQKTQWVNG"
BASE COUNT          406 a          615 c          563 g          412 t
ORIGIN      
        1 gtcagcagct ccatgcggat cagtccggcc atggcgccgc cgatgaagaa gaagaacgtg
       61 atcgagatca gatacagcat cgcgatccgc ttgtggtcgc cggtcagcag ccagctcttc
      121 agaccgtatt ctttattcag atacgagacc ttcggcagtt tggctgtcga ctggtccggc
      181 agtgtaacga ttgtgctcat ggcttcaccg tcccgggtgg aagcgccgga gccgatgtcc
      241 ccggcggcgt agcgccagtc tgaatgggct gggccgcgct gttgtcgatc gtctgctcga
      301 tccggtactg cgaattcagc tgccggatgt actccactag atcaatcagc ccttcttcgc
      361 tcacctgccc ctgataggtc ggcatgatgg gcgcgtaccc ggccacttcg tgcagggtcg
      421 ggttcagaat cgtgtcgcgc aggaaggcat cgtccaccgt cgcaaactgc ccattcttca
      481 gctgcaggcg cgaaccgtaa atgttggcca gattcggacc gtgcgcgttc gggctgctgt
      541 tgtggcagga attgcagccc aggctcgcga acagccgttc tccgttctgc gccagagact
      601 gcccgctggt cgatcccgct gcccacgcct ggtagtcttc cggcgtcatc gctgtcactt
      661 cgccgatcat ctgcgagtgc agtgtgccgc agtactgcgt gcagaagatg tgatacgtcc
      721 cgggtatggt cgcttcaaac cattccgtgt tgtagcgtcc cggaatggcc tcgcgtttca
      781 cgcgaaacgc cgggatcgag aagctgtgga acacgtcctg cgagatcagc gtgatctgca
      841 ccggcttgtt aatcggaata tgcagggcgt tgatttcatg ctgcccgcct ggatgctcca
      901 ccttccacat ccactgcttg cccaccacat aaatgttcat cgcattcggc ggcggactgt
      961 agatccggta atacaacagc gcgccccaca cgaagcagac catgaacagg atgaggggaa
     1021 tgatggtcca ggtcgcctcc agcagcgtcg acccttcaat cggcgtggca acgggatgca
     1081 aatcgcgacg atagcggatg gagaaggtga cgaccaggag cgcgacgatt cccagtccca
     1141 ccaccgagat cgaaaccaga aaaaagtaaa gcgcatccat ctccgccgct ttgctcgagg
     1201 cttccggcgg gaagatcgcg aaatacgtaa gccatttaat taaaaactgc caaagtaccg
     1261 gactgatgaa atgcatcgtc tatccgttca cccattgcgt tttctgttcg cgcgcctgcc
     1321 gtatatcgcg ccggaagttc accagcataa aagttcccag agccagcacc gtcagaatcc
     1381 agcccagctg gaagatgcga atgtaaagca gcgtgtgccg gttggctgtc ggatcgtact
     1441 gatagcagta cgtcagcaag ttatcgacca gggatccgat tttgccgtgc gaggcctcga
     1501 ccagccccag ctgaatatcc ttcggcgaaa actccacgcc catgtagtac tgcgccagct
     1561 tcccttccgg cgtcaccagc tcgatggcgc tggcatgcgc aaactgcgtc atcttgccgt
     1621 cgggcccctg catcggcaca tagccgaatc ccaccgcagt cgccagagcg cgaatcgccg
     1681 gctcctgtcc ggtgaggaaa tgccagccgt tgtcgtcctg cggccggccg aagcgattca
     1741 tgtagaacgc cttctcgcgt gccgcgatcg ccggggtctc cttcgcatcg atgctcacca
     1801 gaaccacgtc gtagtcgcgg ctgggattga acttcagcat ctccagcgcg cccgtcaacc
     1861 catcgatctc ttccgggcaa agaatcttgc actcgtagta gaccagcgac aggatcaccg
     1921 gccgctttcc gaagtagtcg cccagatgaa cggctttgcc cgtctcgtcg cggaactcca
     1981 gattgagcgg cagctg
//
LOCUS       DASZYN010000152         2912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_6789, whole genome shotgun sequence.
ACCESSION   DASZYN010000152 DASZYN010000000
VERSION     DASZYN010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6789"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..785
                     /locus_tag="VHE33_03945"
     CDS             48..785
                     /locus_tag="VHE33_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96633.1"
                     /translation="MLRASLIAGLVVLLALSLAAQQQDNSSQQQNPPSQTTKPAQQKK
                     DQPKKSAAQANPFPQAQSEAAAHQTEHQQNPDAPAPQTAPKADSSGKSADQNPFPEEQ
                     SQKAAHEAQQNKTTPDSAEPPSEPSYSSSHVKGLDMPTTDEQPPGNLPPSQLPYDPKL
                     AHKDANIGDWYMETGNWKGAYDRFLEAHQSDPGNAEAVFGLAESAHHLRMDDVALQNY
                     RLYLSALPNGPHAKEIHKALKQMGAER"
     gene            complement(801..1337)
                     /locus_tag="VHE33_03950"
     CDS             complement(801..1337)
                     /locus_tag="VHE33_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96634.1"
                     /translation="MDRVEPPPLVGTQGAEDEMGGDTLQPKALAALVEQVVHLVDLKA
                     EDIDELGATHGLPVELIGTREAGSGCASWHVAEGKNDDLQHPLNGGAAILRGTTALQT
                     LCNNAAAAPEREDEMFDQLLRGPEPVILTRVHLAPVGGLAVEFGSPGVENLLQDGMHG
                     MAVGTAYAFRGNYTTTRF"
     gene            complement(1471..2664)
                     /locus_tag="VHE33_03955"
     CDS             complement(1471..2664)
                     /locus_tag="VHE33_03955"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HVT96635.1"
                     /translation="MRCTLFPRCYAFALSLLLAAVPLTLCAQGADKSKEPDTLVLSNG
                     DTLHGTLVQETNGTVTFHSDALGDIKLDWEKIQSLHTSQAFAVLGKQMKKRDKHAAEA
                     FPRGTVEVANGLVTLHPENGATIAPIPVKSAQVIVSAPELDKELRHEPGFLEGWNGAA
                     TAGATLVTATQNSYAVSGSVGLVRTVPTVTWLDPRNRTSADFSASFGKITQPSYTSGG
                     VFTPATTTKSSILHFDAERDQYFSPRMFALAQTAFDHNFSQDLDLQQIYGGGLGWTFL
                     KTPIQEADLKATMQYEKQQFIAGSTEANQNLIGSTFSVNYLLKLKLVTYAQELSFIPA
                     YNNPRAYSATETNTLTFPTYKNFGFSVGTLDSYLNDPPLTAPPTRRNSFQFTMGVTYA
                     FKSKY"
BASE COUNT          595 a          986 c          793 g          538 t
ORIGIN      
        1 aatcctcgga accctctcca cacatcagcc gtctaaaata gagaagaatg ctgcgcgcct
       61 ccctcatcgc cggcctggtc gtcctcctgg ccctttctct cgcggcccag cagcaggaca
      121 attccagcca gcagcagaat cctcccagcc agaccaccaa gcccgcgcag cagaaaaaag
      181 accagccgaa gaagagcgcc gcccaggcga atcccttccc gcaggcccag tccgaagccg
      241 ctgctcatca gaccgagcac caacaaaacc ccgatgcccc ggcgccgcag accgcgccga
      301 aagccgattc ctcaggcaag tccgccgatc aaaatccctt ccccgaggaa caatcccaga
      361 aggccgctca cgaggctcag cagaataaaa ccacgccgga ttccgcggaa ccgccgagcg
      421 agcccagtta cagttccagc cacgtcaaag gcctcgacat gccgacgacg gacgagcagc
      481 cgccgggcaa cctgccgccc tcgcagctgc cctacgatcc caaactcgct cataaagacg
      541 ccaacatcgg cgactggtac atggagaccg gcaactggaa gggcgcctac gaccgcttcc
      601 tcgaagcgca tcaatccgat cctggaaatg ccgaagccgt ctttggtctg gctgagtccg
      661 cgcatcatct gcgcatggat gacgtagccc tgcaaaacta ccgcctctac ctcagcgccc
      721 tgcccaacgg tccgcatgcc aaagagatcc acaaagcgct gaagcagatg ggcgccgaac
      781 ggtagcggat tccccggccg tcagaatctt gtggtggtat agttccctcg aaacgcgtac
      841 gccgtgccca cggccatccc atgcatgccg tcctgcagca ggttttcgac tcctggactg
      901 ccgaactcaa cggcaagccc tccgactggt gccagatgca cccgcgtcag gatgaccggc
      961 tctggtccgc gcaggagctg atcgaacatc tcgtcctcac gttccggagc agcagccgcg
     1021 ttattgcaaa gcgtctggag cgcagtcgtc ccacgaagga tcgcagcacc gccgttcagt
     1081 ggatgctgca gatcgtcgtt ctttccttcg gccacatgcc acgaggcgca cccgctccca
     1141 gcttcgcgcg tcccgatcag ctccactggc agcccatgag tggcgcccag ctcgtcgatg
     1201 tcctccgcct tgagatcgac cagatggacg acctgctcga ccagtgccgc cagcgctttg
     1261 ggctgcagcg tgtcgccgcc catttcgtcc tcggcccctt gcgtcccgac cagtggcggc
     1321 ggttccacgc ggtccatcta cgtcaccacc tcggccagct ccaccgtctg gagcaatcca
     1381 tcggccatcc ggcagcacac gaagcccagg cctcctgagc ccgaagccag cgcaggatcg
     1441 cctgctgctg gctatcggct gctggctcgc tcagtacttc gacttgaacg cgtacgtgac
     1501 gcccatcgtg aactggaacg aattccgtcg ggtgggcggt gcggtcaacg gcggatcgtt
     1561 caggtagctg tcgagggttc ccacgctgaa gccaaaattc ttatacgtcg ggaaggtcaa
     1621 cgtgttcgtc tccgttgccg aatatgcccg cgggttgttg tacgccggaa tgaacgacag
     1681 ctcctgcgcg taggtcacca gcttcagctt cagaagatag ttcacagaaa aagtcgaccc
     1741 gatcaggttt tggttcgcct ccgtcgagcc cgcgatgaac tgttgctttt catactgcat
     1801 cgtcgccttc agatcggctt cctggatcgg agttttcaaa aatgtccagc ccagtcctcc
     1861 gccatagatc tgctgcagat cgagatcctg gctgaagttg tgatcgaaag cagtctgggc
     1921 cagcgcaaac atcctcggcg aaaaatattg gtcgcgctcc gcgtcgaagt gcaggatcga
     1981 cgatttggtc gtggtggccg gcgtgaatac cccgccgctg gtatagctgg gctgcgtgat
     2041 cttgccgaac gaagccgaaa aatccgccga cgtccggttc cgcggatcca gccaggtcac
     2101 cgtgggaacc gtgcgcacca gccccacact gccggacacc gcataggaat tctgtgtggc
     2161 ggtcaccagc gtagcgccgg ccgtcgccgc accgttccag ccttccagga agcctggctc
     2221 gtgcctcagt tccttgtcca gttcaggagc actaacgatc acctgcgcgc tcttcaccgg
     2281 aatgggcgcg atggtcgccc cattttccgg atgcagcgtc accaaaccgt tcgccacttc
     2341 caccgtgcct ctgggaaacg cctcggctgc atgtttgtcg cgctttttca tttgcttccc
     2401 cagcaccgca aacgcttgcg acgtgtgcag ggactggatt ttttcccagt cgagcttgat
     2461 atccccaagc gcatcgctgt ggaacgtaac ggtcccgttc gtttcctgca ccagcgtgcc
     2521 gtgcagcgta tcgccgttgc tcagcacgag ggtatccggt tccttgctct tatctgcgcc
     2581 ctgcgcgcat aaagtgagcg gcaccgcggc cagcaacaac gaaagagcga acgcgtaaca
     2641 gcggggaaaa agcgtacaac gcatggacga gaagtcctcc tcaagaatat gaaccggaaa
     2701 atgaccgttg gctttctgca attcaagatg cggggagcgc ggcctggcgt tgcagccgcg
     2761 ctggacgcca cacccgaatc tccctgacaa tgaaactatg ccgattaccc gtgaacaggc
     2821 cctggactac ttccgctccg acgatctgat cggcctgggc atggaagccg acgccgtccg
     2881 caagcgtctc cacccggagg gcgtcgttac gt
//
LOCUS       DASZYN010000153         3662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_70681, whole genome shotgun sequence.
ACCESSION   DASZYN010000153 DASZYN010000000
VERSION     DASZYN010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70681"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /gene="zapA"
                     /locus_tag="VHE33_03960"
     CDS             <1..328
                     /gene="zapA"
                     /locus_tag="VHE33_03960"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HVT96636.1"
                     /translation="FSMPDENAITVDIYDQTYRLNGNDRDYLERLAATVDGKMRAVAS
                     QGRSVDSLRVAVLAALNLADELARLEERYAAVTSAGDSVASIRSRAHSLSGMLDEVLS
                     EERKAG"
     gene            complement(391..3207)
                     /locus_tag="VHE33_03965"
     CDS             complement(391..3207)
                     /locus_tag="VHE33_03965"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HVT96637.1"
                     /translation="MKTARVTFICLFALISAFPAAALGQTAHKLPTIQYKKYTLPNGL
                     TVITHEDHRLPLVAVDLWYHVGPLNERPGRTGFAHLFEHMMFEGSEHVGEKAHIRDVQ
                     AAGATDVNGTTSFDRTNYFETMPSNQLELALWLESDRMGFLMEGLDREKLTNQRDVVR
                     NERRQGEGRPYDVEDEAVMHTLFPKGHPYYGNVIGSHADIESARIADVRDFHQQFYTP
                     NNASIAIAGDFNPEQLHTLLTKYFGPIPSGPKVAPVTATTPKITSQRRVTVTDTVQLP
                     QFTVAWLTPPLFSPDSYSLHAAMYAFGEAKVSRLDDALVYKAQTAQDVSCGSENLKLT
                     GITECSITAKPGVKLEDLEASFWSILAKLQQEGPAPEEVEASKSLTLTQKISGLQRLG
                     GFGGVADTLDMYNQYLGDPGYLPKDVAMTEAVTVASAKAAANKYLTKNSAVVVYCVPG
                     KKVLDDVPRSPADTDANVKITNPYTPEFEKAQDWRKTKPEAGPAPALHLPVPVEFSLD
                     NGLKVLLVEDHALPVITADLVSRAGNANNPLDKSGLASLTAEIMSDATTSRDVKKLSS
                     DEEQIGARITAQSGMDSAQISTSLLTWHADAGLELLADIAEHPAFHADDIDRDRKQRL
                     VSIAQQSDNVASMAFRVGPKLVFGDSPYGLTSTGTTESVNAIKADDISGFYANHYGPQ
                     DAALVLAGDLTRAQAEELARKYFGKWTASNVKSAAIPAPPAPQPTHIVIVDKPGAPQT
                     ALFAFGLGVPEGSPDENALNVMNYTLGGSFASRINMDLREKHGYTYGASSRFASYQDG
                     GQFYAGGLVRTDVTPPAAKDLMTEIRNFPTNPSTAEELDGAKEASMRSLPGRFQTTSI
                     IANAMTEIFLYHRPLDYYAQLPSKYEAVTQADVNRVAQQYLHPDQLVIVAAGDRSKIE
                     PGLKEAGLGPVEVRDIEGKLVDQK"
     gene            3412..>3662
                     /locus_tag="VHE33_03970"
     CDS             3412..>3662
                     /locus_tag="VHE33_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96638.1"
                     /translation="MKKATLLLLVSLALPAFAQNQKHPTTLKGILLDQLHSTHDQAQW
                     FVPAKVAVAGLTAEQANWTPGKGQHSVGQLAYHLWYWDNR"
BASE COUNT          628 a         1052 c         1221 g          761 t
ORIGIN      
        1 tttttctatg cccgacgaaa acgccatcac cgtagacatt tacgaccaga cctaccgtct
       61 gaacggcaac gaccgcgatt atctcgagcg gctggccgcc accgtggatg gaaagatgag
      121 ggcggttgcc tcgcagggcc gctcggtgga ctcgctgcgc gtggctgtgc tggcggcgtt
      181 gaacctggcc gacgaactgg cgcggctgga agagcggtat gcagcggtca ccagcgcggg
      241 cgatagtgtg gcttcgatcc ggagccgggc gcattcgctg agcgggatgc tggacgaggt
      301 gctcagcgag gaacgcaagg cgggctgaga ggcgcgagtt ccaggcaggt gaatgcgggc
      361 gaggggtcaa cctcctcgcc cgttccgttt ctacttttgg tcgaccagct tgccttcgat
      421 gtcccgaacc tcgacaggac cgagtcctgc ctctttgagg ccgggttcga ttttggatcg
      481 gtcgcccgcc gccacgatca ccagttggtc cgggtggaga tactgctgtg cgacgcggtt
      541 gacatccgcc tgcgtgaccg cttcgtactt cgagggcagt tgcgcgtagt agtccagagg
      601 ccggtggtag aggaaaatct cggtcatggc gttggcgatg atcgacgtgg tctggaagcg
      661 ccccggcaac gagcgcatcg aagcctcttt cgcgccatcc aactcctccg ccgtggatgg
      721 gttcgtgggg aagtttcgga tttccgtcat taagtctttt gccgcgggcg gcgtcacgtc
      781 ggtacgcacc aggccgccgg catagaattg gccgccgtcc tggtacgacg caaatcgcga
      841 cgaagctcca taggtatagc catgcttctc gcgcaggtcc atgttgatgc ggctggcaaa
      901 gctgccgccg agggtgtaat tcatcacgtt cagggcattt tcatccgggg aaccctcggg
      961 gacgcccagc ccgaaagcga acagcgcggt ctgtggagca ccgggcttgt cgacgatcac
     1021 aatgtgcgtc ggctgcggcg ctggcggagc gggaatggct gcggacttca cattgctcgc
     1081 agtccatttg ccgaagtatt tccgggccaa ctcctcggcc tgggctcgcg tgaggtcgcc
     1141 ggcaagaacc agggcggcat cctgcggacc atagtgattc gcataaaacc cgctgatgtc
     1201 gtcggccttg atcgcgttga cgctctcggt tgtgccagtt gatgtgagtc cgtaggggct
     1261 gtccccaaag accagcttcg gaccgacgcg gaaggccatg ctggcgacgt tgtcggactg
     1321 ctgtgcgatg ctgaccaggc gctgtttgcg gtcgcgatcg atgtcgtccg cgtggaaggc
     1381 cggatgttcg gcgatatccg cgagcagttc cagtccggcg tcggcatgcc aggtcaggag
     1441 gctcgtcgag atttgtgccg agtccattcc tgactgcgcc gtgatcctcg ccccgatctg
     1501 ttcttcgtcg gaagagagct ttttgacgtc gcgcgaggtg gtggcgtcgc tcatgatttc
     1561 ggcggtgagc gaggcgaggc cgctcttgtc gagcgggttg ttggcgttgc cggcgcgcga
     1621 aaccaggtcc gcggtgatga cggggagcgc gtgatcctcg acgagcagga ccttcaggcc
     1681 gttgtcgagg ctgaattcca ccggcaccgg caggtgcagg gctggagcgg gtcccgcctc
     1741 gggcttggtc ttccgccagt cctgcgcctt ctcgaattcc ggcgtgtaag gattggtgat
     1801 cttcacgtta gcgtccgtgt cggccgggct gcgcggcacg tcgtccagca cctttttacc
     1861 gggcacgcaa tagacgacca ccgcggagtt ctttgtgagg tacttgttcg ccgcggcttt
     1921 tgcgctggcc accgtcaccg cttccgtcat ggccacatct ttgggcaggt aaccgggatc
     1981 gcccaggtac tggttgtaca tatcgagcgt gtcggccacg ccgccgaagc cacccagcct
     2041 ctgcaggccg ctgatcttct gggtcagggt cagagacttc gacgcttcga cttcctcggg
     2101 tgcgggacct tcctgctgga gcttcgccag aatcgaccag aaggaggcct cgaggtcttc
     2161 gagcttgacc cctggcttgg ccgtgatgga gcactcggta atgccggtga gcttgaggtt
     2221 ttcactgccg caactgacgt cctgcgcggt ctgcgccttg tagacgagcg catcgtcgag
     2281 gcggctgacc ttggcctcgc caaaggcgta catggcagcg tgaaggctgt aggaatcggg
     2341 actgaataat gggggcgtca gccacgccac cgtgaactgc ggcagctgca cggtgtcggt
     2401 gacggtaacg cggcgctgcg atgtgatctt cggcgtcgtc gccgtcacgg gcgcgacctt
     2461 cgggccggag ggaatcggtc caaagtattt cgtcagcaga gtgtggagct gttcgggatt
     2521 gaagtcgccg gcgatggcga tcgatgcgtt gttgggcgtg tagaactgct gatggaagtc
     2581 gcggacgtcg gcgatgcgcg ccgattcaat atcggcgtgg ctgccgatga cattgccgta
     2641 ataaggatgt cccttgggga agagcgtgtg catcacggct tcgtcttcca cgtcgtaggg
     2701 acggccttcg ccctgacgcc gctcgttgcg gaccacgtcg cgctggttgg tcagcttctc
     2761 gcgatcgaga ccttccatga gaaagcccat ccggtcgctc tcgagccaca gcgccagttc
     2821 gagctgattg ctgggcatgg tctcgaagta gtttgtgcga tcgaagctgg tggtgccgtt
     2881 cacgtcggtc gcgccggctg cctgtacgtc gcggatgtgg gctttctcgc cgacgtgctc
     2941 ggagccctcg aacatcatgt gctcaaacaa gtgcgcgaag cccgtgcgcc ccggacgctc
     3001 gttgagcggg cccacgtgat accagagatc gacggccacc agcggcaggc gatgatcctc
     3061 atgcgtgatc acggtgagcc cgttgggcag cgtgtatttc ttgtactgaa tcgtcggcag
     3121 cttgtgtgct gtctggccca gggcggcagc gggaaaggcg gaaatcaaag cgaacaggca
     3181 gatgaacgtg acacgagcgg ttttcaagca aggccctcca gtgtgaccca accagcagca
     3241 gttcgagtgg caactcgggt acaggcgatg gtaaatggat tggcttgatt ccggcgacct
     3301 tcaggagagg ctgcggcgac ctatccgcgt ggcggccatt cgtcactggt tatggagggt
     3361 atcctcgcgg cgtgctatcg ttttgcggaa ttccactgga gaaacccacc gatgaagaaa
     3421 gcgactctgc tgctgctcgt gagccttgcg ctgcctgcat ttgcgcaaaa ccaaaagcat
     3481 cccaccacgc tgaaaggcat cctgctcgat caactccatt ccacgcacga tcaggcgcaa
     3541 tggttcgttc cggcgaaagt ggctgtggcc gggctgacgg cggagcaggc gaactggacc
     3601 ccgggaaaag gtcagcattc ggtaggccag cttgcctacc atctgtggta ctgggacaac
     3661 cg
//
LOCUS       DASZYN010000154         3715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1099243, whole genome shotgun sequence.
ACCESSION   DASZYN010000154 DASZYN010000000
VERSION     DASZYN010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099243"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /gene="mazG"
                     /locus_tag="VHE33_03975"
     CDS             <1..711
                     /gene="mazG"
                     /locus_tag="VHE33_03975"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HVT96639.1"
                     /translation="EETYEVLDAIEREHWSDLADELGDLLLQVLFYAEMAEEAGHFAI
                     EDVIANLNRKLVRRHPHVFGEEASAAAGNRAQGLETNGIGAREVLRNWEEIKKLENKS
                     RLEAAGRLDSVPRAMPALSEAGKIGSKAAKVGFDWPDIHGVIAKLHEECAELEAEIAA
                     GDPKAAGEEMGDLLFTVANLARHLKVDPELALRDANAKFRHRFAGMEAAADQPLENLS
                     AKELEKLWEHSKEAEGRA"
     gene            675..1526
                     /locus_tag="VHE33_03980"
     CDS             675..1526
                     /locus_tag="VHE33_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96640.1"
                     /translation="MGAQQGGRGARVRAETQQGAIRTPAGTDIVIRPCQGLDDLGACV
                     ELQVETWGYNDGDVIPRRMFIVAQRIGGQVIGAFHGEQMVGFAMSLPGVKPGKEPGDN
                     PFSYLHSHMLAVKAEFRNFGIGRKLKLFQRDEALSRGIRLMEWTFDPLEIKNAFLNIH
                     KLGVIVRSYTPNFYGVSSSRLQGGLPTDRLHAEWWLDSERVKAILNGSPCARPEIQET
                     IVVPKEVYEWKASATGVSKATALQAENRQRFLDAFARGLAVVGFTVDQHGNGAFELAR
                     LENSGVL"
     gene            1523..2647
                     /gene="menC"
                     /locus_tag="VHE33_03985"
     CDS             1523..2647
                     /gene="menC"
                     /locus_tag="VHE33_03985"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HVT96641.1"
                     /translation="MRIDAIFLRELRLPLVKPFQTSFGTTTERRILLVELRAEGLTGW
                     GECVAGEHPFFSDETIDTAWLVINQELAPALAAADPEHGGKCPSIFRQVRGHRMAKAA
                     LENAVWDLEAQMKRISLASLLGGTRETIDCGVSIGIQPTLEQQLAEVEKEVNAGYQRI
                     KLKIKPGWDVKVLEAVRNRWPEIQLSVDANSAYRLNDADHIVGWDEFNLLMIEQPLWA
                     DDFYFHSMLQKRLETPICLDESIRNRRDALAAIEMESCRIINIKCGRVGGFSEAIAVH
                     NAAQERGVPVWCGGMLETGIGRAHNIALSSLDNFSLPGDVSASKRYWKEDIIEPEVVV
                     SKTGEIVVPETPGRGYAVRTDLVEKLTVRKEEIRALEMVA"
     gene            2772..3560
                     /locus_tag="VHE33_03990"
     CDS             2772..3560
                     /locus_tag="VHE33_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HVT96642.1"
                     /translation="MKKLALLMVVVSITCAAACAQDSVPGTPMQATPQDGYAAARSLE
                     LYREGNARMLMNDYGQLARYHDDDVKLGPPAPGEKRVIFFGDSITDGWKLDESFPGKN
                     YINRGIGGQTTPQMLVRFREDVINLKPAVLVVLAGTNDIAGNTGDETLEQIEGDYQTI
                     AELAKANGIRLIFSSVMPINNYNQRAMWFFLQRSPEKILALNAWLKKYCADNRLIYLD
                     YFSAMVDGRGMLKADLTQDGLHPNAAGFAVMAPLAEAAIERAEK"
BASE COUNT          778 a         1140 c         1124 g          673 t
ORIGIN      
        1 gaagaaactt acgaagtact ggacgccatc gagcgcgagc actggtccga tctggccgac
       61 gagctcggcg acctgctgtt gcaggtgctg ttctatgccg agatggccga ggaggccggg
      121 cacttcgcca tcgaggacgt gatcgctaac ctcaatcgca aactggtgcg ccgccatccc
      181 cacgtcttcg gcgaagaagc ctctgctgcg gcaggcaatc gcgcccaagg cctcgaaacc
      241 aacggcatcg gtgctcgcga ggtccttcgc aactgggaag agatcaagaa actcgagaac
      301 aaatcccggc tcgaagccgc gggccgcctc gactccgtcc cgcgcgccat gccggcactc
      361 agcgaagcag gcaaaatcgg ctcaaaagcc gcgaaggttg gcttcgactg gcccgacatc
      421 cacggtgtca tcgccaagct ccatgaggag tgcgccgaac tcgaagccga aatcgccgcc
      481 ggcgatccga aagccgccgg cgaggaaatg ggcgacctgc tgttcaccgt tgccaatctg
      541 gcacgtcatc tcaaagtcga ccctgaactg gcactgcgtg acgccaacgc gaaattccgg
      601 cacaggtttg cgggcatgga agcagcggcg gatcagcctc tcgaaaacct gtcggcgaag
      661 gaactggaga agctgtggga gcacagcaag gaggcagagg ggcgcgcgtg agggcagaaa
      721 cacagcaagg tgcgattcga accccggccg ggaccgacat cgtcatccgg ccttgccagg
      781 gtctcgacga cctcggcgcc tgcgtggaat tgcaggtcga aacatggggc tacaacgacg
      841 gcgacgtcat tccgcggcgc atgttcatcg tggcgcagcg gatcggcggt caggtgatcg
      901 gtgcatttca cggtgagcag atggtaggtt tcgccatgtc tttgcccggc gtgaagcccg
      961 ggaaagaacc cggagacaat ccgttctcct atcttcattc gcatatgctc gcggtgaagg
     1021 cggaattccg caacttcggc atcggccgta aactcaagct ctttcaaaga gatgaagcgc
     1081 tctcccgcgg catccggctc atggagtgga ccttcgatcc actcgaaatc aaaaacgctt
     1141 tcctcaatat ccacaagctc ggcgtaatcg ttcgatccta taccccaaat ttttatggtg
     1201 tatcctcatc tcgattacag ggtgggcttc ccacagaccg gctccatgcg gagtggtggc
     1261 tggattcgga acgtgtgaag gcgatcctca acgggtcccc ctgcgcacgg ccggaaatcc
     1321 aggagacaat tgtggtcccg aaggaagtgt acgagtggaa agcttctgct acgggtgtct
     1381 ccaaagccac cgcgctgcag gcagagaatc gccagcgctt tctggatgca tttgctcgcg
     1441 gtctggcggt ggtgggtttc acagtcgatc agcacggtaa cggcgctttc gaactcgcaa
     1501 ggctggaaaa ctccggagtt ttatgagaat cgatgccatc tttttgcgcg aactacgcct
     1561 tcccctcgtt aagccatttc aaaccagttt tggaaccaca accgagcggc gcatcctgct
     1621 cgtggagctc cgcgccgagg gactgaccgg ctggggggag tgcgttgccg gagagcatcc
     1681 ctttttctcc gatgagacca tcgacacggc gtggctggtc atcaaccagg agctggcgcc
     1741 cgcgctcgct gcagctgatc ccgagcacgg cggcaagtgc ccgagcatct tccgccaggt
     1801 ccgcggccat cgcatggcca aggccgcgct cgaaaacgcg gtgtgggacc tcgaagcgca
     1861 gatgaagcgt atttcgctgg cgagcctcct cggcggcacg cgcgaaacca tcgactgcgg
     1921 cgtctccatc ggcattcagc ccaccctcga gcagcaactg gctgaggtcg aaaaggaagt
     1981 caacgccgga taccagcgca tcaagctcaa gatcaaaccg ggctgggacg tgaaggtcct
     2041 ggaagcggtg cggaaccgct ggcccgagat tcagctgagc gtggacgcca actctgccta
     2101 tcgcctcaat gacgccgatc acattgtcgg ctgggatgag ttcaacctcc ttatgatcga
     2161 gcagccgctc tgggccgacg atttctactt ccactccatg ctgcagaagc gcctcgaaac
     2221 cccgatctgt ctcgacgagt cgatccgcaa ccgtcgcgat gcgctcgccg ctatcgagat
     2281 ggagtcgtgc cgcatcatca acatcaaatg cggccgcgtg ggcgggttca gcgaggccat
     2341 cgctgttcac aacgcggcgc aggagcgcgg cgtgcccgtt tggtgcggtg gcatgctgga
     2401 aaccggcatt gggcgcgccc acaacatcgc gctctcctcg ctcgataact tctctctgcc
     2461 cggcgacgtc tccgcgtcga agcgctactg gaaggaagac atcatcgaac cggaagtggt
     2521 cgtgtcgaaa accggcgaga ttgttgttcc ggaaacgccc ggccgcggct acgcagtgcg
     2581 taccgatctg gtcgagaagc ttacggtgcg caaagaggag attcgcgcgc tggagatggt
     2641 ggcctgacgg tacggcatag catttaagcg aagaggtgag aagaagggga gcggcgatgc
     2701 tgctcccctt ttttgcctcg cgggatcgga gccatgcgaa ccatcccttc tcgaagtgat
     2761 agcttgttgc catgaaaaaa ctggcgctcc tgatggtcgt tgtttccata acctgcgctg
     2821 cggcatgcgc tcaggattcg gtacccggca ctccaatgca ggcgactccg caggacggat
     2881 acgcggcggc ccgcagcctc gagctctatc gcgagggcaa cgcccggatg ctgatgaatg
     2941 actacggcca gctcgcccgc taccatgacg acgacgtgaa actcggcccg ccggcccccg
     3001 gcgaaaagcg cgtgatcttc ttcggcgact ccatcaccga tggctggaaa ctcgacgagt
     3061 ccttccccgg caagaactat atcaaccgcg gcatcggcgg acagaccacg ccgcaaatgc
     3121 tggtccgctt tcgcgaagat gtcataaatc tcaagccggc tgtcctggtc gtgctcgccg
     3181 gcaccaacga catcgccggc aatacgggtg acgaaacgct ggaacaaatc gagggcgatt
     3241 accagacgat tgccgaatta gcgaaggcca acggcatccg cctcatcttc tcctcggtca
     3301 tgcccatcaa caactacaac cagcgcgcga tgtggttctt cctccagcgt tcgccggaga
     3361 agatcctcgc cctcaatgcc tggctgaaga aatactgcgc tgacaatcgg ctcatttacc
     3421 tggactactt ctcggcaatg gtcgacggcc gcggcatgct gaaggcggac ctgacccagg
     3481 acgggctcca cccgaatgcc gccggattcg cggtcatggc gccgctggct gaggcggcga
     3541 tcgagcgtgc ggagaagtag cgcttcgaac cagaaagctg ggaggcgcta tctgctgctg
     3601 ccctgcagat cgtccagtgc caggttcagc tccagcacgt tgacgcgcgg ctcgccgagg
     3661 aagccgagct ggcggccctg gatgtgcgac tcgacaagcg cccgcacggt ggcgt
//
LOCUS       DASZYN010000155         3759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_388564, whole genome shotgun sequence.
ACCESSION   DASZYN010000155 DASZYN010000000
VERSION     DASZYN010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388564"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="VHE33_03995"
     CDS             <1..955
                     /locus_tag="VHE33_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HVT96643.1"
                     /translation="IIATGGDGGNTLNAFNPASVPAAPTIVPAAGIFPGILYAVTLFI
                     GFESAASLGEETRNPRRSIPLAVIGTVIITGVFYLLMVYASDIGFGLGHADKWATDPA
                     PLDTLATRYVGSWLAVLVDIAVLFDAIVVMSAFMATTSRGLFALARHHLLPAPLAGVS
                     QRFRTPLGGIILVGIVAVLVTVVVQLTQLSSASDAIPVNVLTIFSTMSTIGSLLIELI
                     YIGLCVAAVFLLIKEPGKWWRWIFLLVAVVTPILGIYGSVVPFPPFPQSLAVYIVIAV
                     VALSLIWMLVNKFAFPARLGTASEPHPWEADELDEVTNSAE"
     gene            1253..3172
                     /locus_tag="VHE33_04000"
     CDS             1253..3172
                     /locus_tag="VHE33_04000"
                     /inference="COORDINATES: protein motif:HMM:NF008559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HVT96644.1"
                     /translation="MAVPSVTHALEPLTPEEIARAVALVKAAPQYSEHVHFVLVKLHE
                     PPVSAAVVAEEPGALVPREVFLSLLEKVNGEGKAYEVIVNLSEEKVASWQHIPGVQPS
                     IFFDEFFPCEDAVKAHPDFQAALARRGITDLTRVRVDPWSAGNYGRPEEEQRRILRTT
                     VHYQLNPDDPEENSYAHPVAGLHAVLDTNTMEVVRIDDFGVVPIPQGSANYLPKDVGA
                     LRTDLKPISITQPEGPSFTIEGYRVKWQKWDFRIGFSPREGLILHNLYYDHRPLFRSV
                     RIAEMVVPYGDPSLDHCFQNAFDVGEYGLGWLANSLELGCDCLGEIRYFDAHMTENNG
                     SVRTIKNAVCLHEEDDGLLWKHTNFRTGHVEVRRSRRLVVSFIATVGIYEYGFFWYLR
                     QDGSLEMDIKLTGIMNIGAVPPGVEPEYGELLTTDGLYAPVHQHTFCFRLEPMLDGLR
                     NSIEEHDTVAEARATPYGNAFKVVKRQLRTEQEARRDSAPQHARTWRIFNAHQLNPAN
                     GKTVGYQLIPHTHVLPFASSDSSILRRAGFTAHHLWVTPSSSREQYPAGDYPNQHAGG
                     DGLPAWTAANRSIEDTILTLWYNVNVQHNPRLEDWPVMPVTHAGFLLRPANFFAANPA
                     LDVPPPTAAHDCHCE"
     gene            3271..3546
                     /locus_tag="VHE33_04005"
     CDS             3271..3546
                     /locus_tag="VHE33_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96645.1"
                     /translation="MVVVFAREKRAIADKNSIAFFTKKCYAYFVEKTINANIDFPFLL
                     SQATRPGKSGPALPALCRQRIGTVGARIYRLAFCSGDSEDGSAHAAV"
BASE COUNT          688 a         1028 c         1162 g          881 t
ORIGIN      
        1 gattatcgcg actggcggcg atggcggcaa tacattgaat gccttcaatc ctgccagcgt
       61 gccggcggcg ccgacgattg tgccggctgc cggtatcttc cccggtattc tctacgcggt
      121 tacgctgttt atcggctttg agagcgcggc gagcctgggc gaggagacgc gcaatccgcg
      181 ccgctcgatt ccgctggcgg tgattggcac ggtgattatc acgggggtgt tttacctgtt
      241 gatggtctac gcgtccgata tcgggtttgg tctgggtcat gcggataagt gggcgaccga
      301 tccggcgccg ctggatacgc tggcgactcg ctatgtcggt tcctggctgg cggtgctggt
      361 ggatatcgcg gtgctgttcg atgccattgt ggtgatgtcg gcttttatgg cgacgacttc
      421 acgcggtctg ttcgctctgg cgcgtcatca tctgctgccc gcgcctctgg ccggcgtttc
      481 tcagcgtttc cgcacgccgc tgggcggtat catcctggtc ggtatcgttg ccgtgctggt
      541 gacggtggtg gtgcaattga ctcagttgag ttctgcatct gatgctattc cggtcaatgt
      601 gttgaccatc tttagcacca tgagcacgat tggcagcctg ctgatcgagc tgatttacat
      661 cggcctgtgt gttgccgctg tcttcctgct gatcaaagag ccgggcaagt ggtggcgctg
      721 gatcttcctg ctggtggcgg tggtgacgcc tattctgggc atctacggca gtgtggtgcc
      781 gttcccgccg ttcccgcaga gcctggccgt ctatatcgtg atcgcggttg tcgcgctttc
      841 gctgatctgg atgctggtca ataagttcgc gttccccgct cgcctgggta cggcctccga
      901 gccccatccc tgggaggcgg acgagcttga cgaggtgaca aactcggctg aataaacgcc
      961 cggcattaca ccgccttgag gcgctgctcc agggactatc agacccgcga gcgcgtgcga
     1021 gccttgcctc tcacgccttc gcttctgtag cgacggagca gcgcctctgt cgtcttttac
     1081 cagcgaatta cgctgatacg ctaaatcgtg tttgtacatt aaatcaggtc tctattcgat
     1141 accattggga atgccacggg aagaggcaaa gactggacgc gagaaggtgg agctgaccac
     1201 cgggacgttc ccaacggtat ctctattcag gaagggatat tggcagaata gtatggctgt
     1261 tccatctgtt actcatgctc tggagccgtt gactcccgag gagattgcca gggcggtggc
     1321 tctggtgaag gctgctcctc agtattcgga gcatgtgcat tttgtgctgg tcaagttgca
     1381 tgagccgcct gtttctgcgg cggtggtggc tgaggagcct ggcgctttgg tgccgcgcga
     1441 ggtatttctg tcgctgctgg agaaggtcaa cggcgagggc aaggcttacg aggtgatcgt
     1501 caatctcagc gaggagaagg tggcttcgtg gcagcatatt ccaggggtgc aaccttcgat
     1561 tttctttgac gaatttttcc cctgtgagga tgcggtcaag gcccatcccg acttccaggc
     1621 ggcgctggcc cgacgtggca ttaccgatct gacccgcgtg cgcgtcgatc cctggtccgc
     1681 cggcaattat ggccgcccgg aggaggagca gcgccgcatt ttgcgcacga cggtccatta
     1741 ccagctgaat cctgacgatc cagaggaaaa tagctatgcc catccggtgg ctggcttaca
     1801 tgctgtgctg gacacgaata cgatggaagt ggtcagaatt gatgatttcg gcgtggtgcc
     1861 gattccccag ggtagcgcga actatctgcc caaagatgtg ggagccttgc gcacagacct
     1921 caagccgatc tcgattacgc agccggaggg gccgagcttt accattgagg gctaccgggt
     1981 taaatggcag aagtgggatt tccgcatcgg cttttctccc cgcgaggggc tgattctcca
     2041 caatctctac tacgatcatc gcccgctttt tcgctctgtg cgcatcgcgg agatggtggt
     2101 gccctatggc gatcccagcc tggatcactg cttccagaac gctttcgacg tgggcgagta
     2161 tggcctgggc tggctggcga acagcctgga actgggctgt gactgcctgg gcgagatccg
     2221 ctattttgac gcgcatatga cggagaataa cggttcggtg cgcacgatca aaaacgcggt
     2281 ctgcctgcat gaagaggatg atggtctgct ctggaagcat accaattttc gcaccgggca
     2341 tgtcgaggtg cgacgttcgc gccggctggt ggtttcgttt atcgccacgg taggcattta
     2401 tgagtatggc tttttctggt atctgcgcca ggatggaagc cttgagatgg atatcaagct
     2461 gaccggcatt atgaatattg gcgcagttcc tccaggcgtg gagccggaat acggcgaact
     2521 gctgacgact gacggtctct acgctcctgt ccatcagcat accttctgtt tccgcctgga
     2581 gccgatgctg gatggtctgc gcaactcgat tgaggagcat gatactgtcg ctgaagcccg
     2641 cgcgacccct tacggcaatg cctttaaggt ggtgaagcgc cagttgagga ccgagcagga
     2701 ggcgcggcgc gactccgctc cccagcacgc gcgcacctgg cggatcttta acgcgcatca
     2761 actcaacccg gctaatggca agacggttgg ctatcagctc attcctcata cgcatgtttt
     2821 gcctttcgcc agctctgatt ccagcattct gcgccgcgcc ggtttcacgg cccatcacct
     2881 ctgggttacg ccgtcctctt cccgtgagca gtatcccgcc ggtgattatc ccaaccagca
     2941 cgccggtggc gacggtttgc cggcctggac ggctgccaat cgcagtatcg aggacaccat
     3001 cctgacgctc tggtataacg tcaatgtgca gcacaatccg cgtctcgaag actggccggt
     3061 gatgccggtt acgcacgcgg gcttcctgtt acgtcccgcg aacttcttcg ccgctaaccc
     3121 ggctctggac gtacccccgc ccacggctgc ccacgactgc cactgcgagt aggattagca
     3181 gtcatcccgc aggcgatcct gctgggatga ctgctatagt agcggtgaat gggcgggaca
     3241 gcgcgggagc gtgagaagga ctgccgctgg atggtggttg tttttgcacg ggagaagcgg
     3301 gcaattgctg ataagaattc cattgcgttc tttacgaaaa aatgctatgc ttactttgtt
     3361 gaaaaaacaa tcaatgcaaa tatcgatttc ccctttctcc tgagccaggc aacgcgaccg
     3421 ggcaaaagcg gaccagcgct gccagccctt tgcagacaga ggataggaac ggttggtgcg
     3481 aggatctacc gcctggcttt ttgcagcggg gactcggagg atggctcggc acatgcagcg
     3541 gtctgatgtt ggatggtatt gcttttaaaa cggtaaaaag atggaaaatc agcaagcagt
     3601 tacctggcgg gaacttcggg gcaaactcac ggtgaatgta cgggaaaggc ggcgtctggc
     3661 cgaggcggca agtgtgacgg tcgcgacgtt gcagcgctgg gtcaaggaac atacgatgcc
     3721 ccgcgaggag cacctggccc atatgttgtc cgctctgcc
//
LOCUS       DASZYN010000156         1998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_518145, whole genome shotgun sequence.
ACCESSION   DASZYN010000156 DASZYN010000000
VERSION     DASZYN010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518145"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..1332
                     /gene="dgt"
                     /locus_tag="VHE33_04010"
     CDS             232..1332
                     /gene="dgt"
                     /locus_tag="VHE33_04010"
                     /inference="COORDINATES: protein motif:HMM:NF002326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HVT96646.1"
                     /translation="MSQRAFPEPDHPYRTPFQRDGGRIIHSRAFRRLAGKTQVFTQRG
                     SDHFRSRLTHTMEVAQIARTIARALGLNEDLTEALALAHDIGHPPFGHAGERALDEAL
                     QQHGLRFDHNLHALRIVEHFENRHLDFRGLNLTLAVREGIVKHSRDYAATEHPELAEY
                     FLDQKPPLEAQLIDLADEIAYLTADLDDGVEAEILTLPQIRAGVQLFESHFAPIAQQH
                     PAAPVKLLIQETLKQMLNALADDLVATIRRQVAENDLLDLNSVRNAPRRVATFSTEME
                     QLRRQAKDFLYQNLYLCPDLRRAHTQASNVISDLFAVWMADPALLPANYLSQIETESA
                     PRVIADYLAGMTDHFVLETHRKVRKTVVASRS"
     gene            1424..1705
                     /locus_tag="VHE33_04015"
     CDS             1424..1705
                     /locus_tag="VHE33_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96647.1"
                     /translation="MALLVVHPWAQFFAGMLIGCWVGAAVACAGLLLFIGRKVRQLES
                     INSILRKKLKARASPLSVSTGETARMLVMPRPDSIPRMESPLARIAQLN"
     gene            1730..>1998
                     /locus_tag="VHE33_04020"
     CDS             1730..>1998
                     /locus_tag="VHE33_04020"
                     /inference="COORDINATES: protein motif:HMM:NF014015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96648.1"
                     /translation="MRRKLLLSWSSGKDSAWALHRLQLDGSFEVAGLLTTLNAAFDRV
                     AMHSTRRALVEAQAAAAGLPLFTVPLPWPCSNQHYESAMRTACDAA"
BASE COUNT          392 a          698 c          554 g          354 t
ORIGIN      
        1 gcccagtggg agctctccct caatgaattc gcccatacgg tggaagccga catggacgcc
       61 ggagatgtga gcaaggtgca aaagaaactc gacagcgccc gcgttcgcct ggcccgagag
      121 tcgggaagcc cgacggcagg caagcccgag tagcacgcaa tgaagcgata aactgctctc
      181 atggcagaga gggcgttgcc ggccgcgtgc gcggttcctc aacatggacc gttgtcgcag
      241 cgagcattcc ctgagccgga ccacccgtac cggactccgt ttcagcgtga cggcggtcgc
      301 atcatccact ccagggcctt ccgcaggctc gccggcaaga cccaggtctt cacccagcgc
      361 ggctccgacc acttccgcag ccgcctgact cacaccatgg aagtcgcgca gatcgcgcgc
      421 accatcgcgc gagcgctcgg cctcaatgag gacctcaccg aggccctggc tctcgcccac
      481 gacatcggcc atcccccctt tggacacgcc ggcgaacgcg ccctcgatga agccctgcag
      541 cagcacggtc tgcgtttcga ccacaacctc cacgcgctgc gtatcgtcga gcatttcgag
      601 aaccgccatc tcgacttccg aggcctcaat ctcaccctcg ccgtgcgaga gggaatcgtg
      661 aagcattccc gcgattacgc cgccaccgag catccggagc tcgccgagta cttcctggat
      721 cagaagcctc ctctggaagc ccagctgatc gacctcgccg acgaaatcgc ttacctcacc
      781 gctgacctcg acgacggcgt cgaggctgaa atcctcacct tgccgcagat tcgagctggc
      841 gttcagctct ttgaaagcca cttcgcgccc atcgcccagc aacaccctgc cgccccggta
      901 aaactcctga tccaggaaac cctgaagcag atgctgaacg ccctggccga cgacctcgtc
      961 gccacaatcc ggcgccaggt cgccgagaat gacctgctgg acctgaacag cgtccgcaac
     1021 gcgccgcgca gagttgccac gttttccaca gaaatggagc agctccgccg ccaggcgaag
     1081 gacttcctgt accagaatct ctacctctgc cccgacctgc ggcgcgccca cacccaggca
     1141 tcgaacgtca tttccgacct gtttgccgtc tggatggccg atccggcact cttgcccgcc
     1201 aactatctga gccagatcga aaccgagagc gctccccgcg tgattgccga ttatcttgcc
     1261 ggcatgactg atcactttgt gctcgagacg catcgaaagg tcaggaagac ggtggtcgca
     1321 agccggtcat aaacccagag atgtcctcgg ccccatatct gctgtaggtg gtccgtccgg
     1381 ccggcttaga tgaaactgcc gcttcagaaa gcgaggagag ggcatggcac tgctcgttgt
     1441 gcatccctgg gctcagtttt tcgccggaat gctgatcggg tgctgggtgg gagcggcagt
     1501 tgcctgcgcg ggtttgctgc tcttcattgg ccgtaaggtg cgccagctcg aatccatcaa
     1561 ctccatcctg cgcaaaaaac tcaaagcgcg cgcgtccccg ctgtctgtga gcaccggaga
     1621 aacggctcgc atgctcgtga tgccgcgtcc tgattccatc cccaggatgg aatcgccgct
     1681 ggcacgcatc gcgcagctga actaagcacg tctgcgataa gctccagaag tgagacgaaa
     1741 acttcttctc tcctggagca gtggcaaaga cagtgcctgg gcgcttcacc gcctgcagct
     1801 ggacggcagt tttgaagttg ccggactgct gaccactctg aacgccgcct tcgatcgcgt
     1861 ggccatgcac agcacccgcc gcgccctcgt ggaagcccag gctgccgccg cgggattgcc
     1921 gctgttcacc gtccccctcc cgtggccctg ttccaaccag cactacgaat ccgctatgcg
     1981 aaccgcctgc gacgccgc
//
LOCUS       DASZYN010000157         1995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_388574, whole genome shotgun sequence.
ACCESSION   DASZYN010000157 DASZYN010000000
VERSION     DASZYN010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388574"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VHE33_04025"
     CDS             complement(<1..336)
                     /locus_tag="VHE33_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96649.1"
                     /translation="MKAVSRSGGGGRSCANGLRGIFVFAWVLGILVAGTPAGGSSALP
                     TLRTVREAHTLRFEEARRGYPVHLARAQVTFYQAEVPLLFLQDSTDGIFVHMRGQPPA
                     SLRCGDIVAV"
     gene            complement(522..1388)
                     /locus_tag="VHE33_04030"
     CDS             complement(522..1388)
                     /locus_tag="VHE33_04030"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HVT96650.1"
                     /translation="MTSGISRRSFVVSGALASAAAMVPVSSLAEPPAQGESSSGPIRL
                     GLASYTFRNFTRDKMIGFMKELRLTELNAKDAKDHLPMDPAAEAQAVSDYTANGIHLH
                     AAGTIYFREAAEDDIRSKFEYVKRAGIKVIVAGDPTPETLPRIEKFVKEYDVRLAIHN
                     HGPEDKLWPSPLDILKDIKGMDPRIGCCIDVGHTVRAGTDVVEAIHKVGARLFNMHVK
                     DLTDFHDKDSQVAVGDGKMPFRAIFEALIAQKYQGFVDLEYEIHGDDPMPGVTKSYAY
                     MRGVLAGMGHPA"
     gene            complement(1441..>1995)
                     /locus_tag="VHE33_04035"
     CDS             complement(1441..>1995)
                     /locus_tag="VHE33_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96651.1"
                     /translation="WALGVDYPNRITAAGGRYAYKDDWQFYDTLVTNFEYGDKLIAWD
                     SRSCNGMRVYGRERGSTILGTTGSVLVDRDGYEVYDLNGHKTDEFRVKKDAATSSADL
                     VGRDSMTNDHFANFIAGVTHGEKLRAPVEIGNVAVTMLQLSNVAWEVQRELKLDPTDG
                     KIVGDSEAMKGWGREYEKGWEPHL"
BASE COUNT          366 a          688 c          550 g          391 t
ORIGIN      
        1 caccgcgacg atgtccccgc atcgcagcga agcgggaggc tgccctcgca tgtggacaaa
       61 gattccgtcc gtcgagtcct gcaggaagag aagtggcact tccgcctgat agaaagtcac
      121 ctgagcgcga gccagatgga cgggatatcc tcgcctggct tcttcaaatc ggagcgtgtg
      181 cgcctcgcgg accgtgcgca acgtgggcag cgcactcgag ccccccgccg gcgtccccgc
      241 caccaaaatc cccagcaccc aggcgaagac gaaaattccc cgcagcccgt tcgcacacga
      301 gcggcccccg ccacctgacc ttgagacggc cttcattctc cgcgcgctct gcagcacgcc
      361 ttcgcgtgct tgtctgcagc ctttatcggc taaagccctg cagagtatag gacgtgcagt
      421 cgcggagcaa acaaggaaac accggctccc tcaaccgccg ctgtgtcttc cgtaacggta
      481 cttccgatac gtcagaaaca atggcagcgc atgtggcgcc gctacgccgg gtggcccatg
      541 cccgcaagaa cgccgcgcat gtacgcgtag ctctttgtga cccccggcat cggatcatcc
      601 ccgtgaattt cgtattccag atcgacgaag ccctggtact tctgtgcaat cagcgcctcg
      661 aagatggcgc ggaacggcat ctttccatcc ccaaccgcca cctggctgtc cttatcgtga
      721 aaatcggtca gatccttcac gtgcatattg aagagccgcg ccccgacctt gtggatcgcc
      781 tccaccacat cggtgcccgc tcgcacggtg tgtcccacat cgatgcagca gccgatgcgc
      841 ggatccatcc ccttgatgtc cttcaggatg tccaggggcg aaggccacag cttgtcctcc
      901 ggcccgtggt tgtggatcgc cagccgcaca tcgtattcct tcacgaattt ctcgatgcgc
      961 ggcagcgtct ccggcgttgg atcgcccgcg acgatcacct tgatcccggc tcgcttcacg
     1021 tactcgaact tgctccggat atcgtcttcc gccgcttccc ggaagtagat cgtccccgcc
     1081 gcatgcagat ggatgccgtt ggccgtgtag tccgacaccg cctgggcttc ggcagccggg
     1141 tccatcggca aatgatcctt cgcgtccttc gcgttcagct ccgtgagccg cagctctttc
     1201 atgaagccga tcatcttgtc gcgcgtgaag ttgcgaaacg tgtagctggc cagccccagc
     1261 cggatcggcc ccgaagacga ttcgccttgg gcgggtggct cagcgagtga agaaaccgga
     1321 accatggcgg cggccgaggc gagcgccccc gacacaacga aactgcgacg cgagatacca
     1381 gaggtcatgg aattgccccg tgctgctgaa ttttgcttct gatccgttcc caaagccgcc
     1441 ctacaaatgc ggctcccatc ccttttcgta ttcgcgaccc cagcccttca tcgcttccga
     1501 gtcgccgacg atcttgccgt ccgtgggatc gagcttcaac tcgcgctgca cctcccaggc
     1561 gacgttcgaa agctgcagca tggtcaccgc gacattgccg atttcaaccg gagcgcgcag
     1621 cttctcgccg tgcgtaaccc ccgcgataaa gttcgcgaag tggtcgttgg tcatcgagtc
     1681 gcgaccgacc agatcggccg acgacgtcgc agcgtccttc ttcacgcgga actcatccgt
     1741 cttgtggccg ttcaggtcgt acacctcata gccgtcgcga tcgaccagaa ctgaccccgt
     1801 cgtccccagg atggtggagc cccgctcgcg cccatacacg cgcatgccgt tgcagcttcg
     1861 gctgtcccag gcgatcagct tgtcgccgta ttcgaagttg gtcaccagcg tgtcgtagaa
     1921 ctgccagtca tccttatagg cgtagcggcc acccgcggcc gtgattcggt tcggatagtc
     1981 aacgcccagc gccca
//
LOCUS       DASZYN010000158         8768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_582797, whole genome shotgun sequence.
ACCESSION   DASZYN010000158 DASZYN010000000
VERSION     DASZYN010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8768
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582797"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..2434)
                     /locus_tag="VHE33_04040"
     CDS             complement(176..2434)
                     /locus_tag="VHE33_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 - glycopeptide
                     alpha-N-acetylgalactosaminidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-alpha-N-acetylgalactosaminidase family
                     protein"
                     /protein_id="HVT96652.1"
                     /translation="MQCDPERFSRRHFLQWSAAGAAAAGAGWLTPNGEAQEQIADGER
                     HAPEHPLVLSSSQLEVTLDAASGLPWKYRLSSNNAELWGETFGAPVAVTLCRTEPWSF
                     QTLPLTPENHHATHSSVSFRFAPLFGGSPAAHFTLHYRIDGPTLFVEMDAIEESAPFQ
                     LVDVALPGLVTVRESDEGAWLAHGDEGGSLAMLAQAKPGSLPPNRFWGKVLGTLPVVM
                     TGTARALCVQETTSYMDGTTLAVTGDAGSRRAAMGTIKVHRVNGSACYDLNLEHGAPL
                     NCGNQNTPNLLVDQKSSCRLDFLPVTGDPAKAWIAAGQLVRSRLPAIPTHFYDDKYVY
                     GIRCDEPLFPAPSATFEQCHEIVRRVSALIDRSPQVVHLWGWQFRGKDTGYPAVNVVN
                     ERIGGYDGMMRLMEQGEALNATITLSDNYDDAYRSSPAWNEAIIARRPDGQLWKSRAW
                     TGEQSYILGLAKYMEGPGPERIRYTCERYRLRHTTHVDVLSYYSIRNDWDPQHPASGV
                     RNLIDGRYKVLQDFAQHGVDVSSEALRYPMIGHISCFWYLTGPAPCPFGGTAIPLVPL
                     IYGNSAVWGLSGAGARGDAATVRIHRRFYGACAHSILRADTDPAEFLDAFYLDMVPFF
                     VLRGRSVRDFERTGDETRIHFDGDVLIESNPVAKTLRIAVGDREILHDDAVYCPLGEG
                     KIACYSVTPRTVAVPVPSGWKRAEVAAAMLYPDRREESAIRWENGSAQIKLEARQPVI
                     LYARRADARLTG"
     gene            complement(2587..6168)
                     /locus_tag="VHE33_04045"
     CDS             complement(2587..6168)
                     /locus_tag="VHE33_04045"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT96653.1"
                     /translation="MRIRILAMVCGLVGLALFAASLQAQQSTGEVHGTISDSTGSIVP
                     GANVTLTSVGTGVSRHTTANSSGYFAFVDVTPGQYTMTVAMQGFKKVALPPFPLVVGQ
                     ILTENETLTVGSAQETVNVNASTEDVMLEKSSSELGSVIETKEIQQLPLNGLNFTSLL
                     ILSPGVNPVSTAQGSGISTTDAGITAIPGTNFYKPSFFGQQNRETYYLMDGIVNTDLR
                     GAIYGFLPIIDAMQEFKVASHIDDAQYGVVTGGVVNMLSKSGTNSFHGSVWEFDRNNL
                     FDARNTFSDFCSVGRCAPGASSTTPAPPGHYVQNEFGGALGGPMWPNRMFFYGAYQGW
                     RYSKPGLSQQLLPTSQELSGDFSNSSYSYYQHPIYNPWSTACTGSKCTVQPFLCDVSG
                     NPITPNSDGTQTGGTPCLKIPAGLINGTMASYLKAYYLPPNAQANEASGYNYIENRSN
                     IDNTDSYQVRVDFHKNDKNFGFGRISQMWAYDTSPVNGTVDANVSHYHAYNFGGGYTH
                     VFSPGLILDVRGGAMLKPYKFRQAAAPGGYAAASSAGFTNLSQYSGMYINLAGPYDTS
                     NAGNDGTRDRGNPVVSAGGSVTWIKGAHTIKAGVDYLYQNRLQRNLYQQFTFSDSTTS
                     NVNASKTGNSLASALLGLPATFTAQQPDFSEDYFSMSLWSGYVQDSWRVNPKLTVNYG
                     LRYDYLPAIHMLNNRLANGLDIFNQKYIIQRNVAACGSTFVNPCIPGGLASVPYSDHI
                     VFANGAEQVGPPIKDNFGPRLGFAWQVRNGTVVNGGAGIFFDTITGRSQWVQNTIEGP
                     TWPWTTGISSQQVNFSQGGVWEGGPGNPLTQITSLEGNFPNPVIASSPWLTTGGGYVN
                     EPGYKDQRAVEYNLQVQQQLAPTTLLTIGYAGSKSTRLDFTGFANAAQHASPAGTPLT
                     TIDSLKAMPWAAPGWHYSEDTGYANYNALLAQFQKRFSNSLNTILSYTWGKSLDNSSG
                     WFSAENGTGGGSVVQSFFLPRNAYGISSYDVRNTLSWSTVYALPFGKQQRWLQSGPLS
                     YVLGGWTTNYLVLMHSGQPYNLNVGGDVANISGDNGSVTSYSRPNVIGDPNHGSCGST
                     PIGKRGPTGFCEYNPSAFSIPSGSFGDMGKMPFRIPFYNDLDLSLVKQTPLYENVSLE
                     LRAESFNLYNAQIIGSPGTTIGNSSAGLANGLSSTPRELQFGAKITF"
     gene            complement(6414..7196)
                     /locus_tag="VHE33_04050"
     CDS             complement(6414..7196)
                     /locus_tag="VHE33_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HVT96654.1"
                     /translation="MKPRKSGTAPVGVLTKVLTIFDLLDRTPGGLQLRSIAQLTKLNK
                     STAWRFLSHLEGAGYVVRDGAGAYLLGPRLVHLGAGSTYQGTICRVSQPILEELWRQT
                     GETVNLGVLDGKEVLYLSVLESPHSFRLVSKAGLRRPLYCTALGKVILAGQPSAVRDE
                     LMAATRFEKLTPRSISRPVELIAELGRIQSRGYAIDNEEVVEGARCIAAPILDCSGYV
                     AAAVSVSGPVSRMARARIPAIAQHVQRAGSRISASLGYKESS"
     gene            complement(7395..>8768)
                     /locus_tag="VHE33_04055"
     CDS             complement(7395..>8768)
                     /locus_tag="VHE33_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HVT96655.1"
                     /translation="GSANGHDYHAVDAIPLSGDSTLEDGNWALLYAVNASNVTIEGPG
                     TIDGQGIQFHAAVRGATPPSGLGGRDRPYHLLLYRCENVRIRDIDLVNSAYHSVRVIQ
                     SRRVHMNGIYIYNRVNGNNDGFHFISCQYVTVSDCTVLSQDDACALFGSCRYVTITNS
                     TFSTRWSCFRFGGGVARDITVSNCVLYQVFGCPIKFHGVPGSRFENMSFSNLVLDEVT
                     GPICISIGPREPRRPGSGPSPVEAVPAENNEPAVARNISFSNIHGTVTTNPPQLPGMP
                     FPSAYRPGELHSCITLNCVGDAVLEKISFENIHLTFGGGGTVEDTARRDLAQIAGEYF
                     MLGPMPAYGLYARNARALTLNNIRFETQTPDLRPALILDRVEDVSIAGLAVTADPDAE
                     SAIRFADTQSVLMTAPRLLVKTGTFLLVEGAQSGGIILDGGDLTRATRQVAFDRGASP
                     SSATVKG"
BASE COUNT         1741 a         2630 c         2621 g         1776 t
ORIGIN      
        1 ctcggcatga gcggactcgg cgaaaccaaa gcggcgcacg aagggaagcg atccggcaag
       61 tgcggaaaca gcggccagaa agccgcggcg cgtgagagag tacatgtcct tcctttggcg
      121 gcgaccgcaa cagcctattc atggacgctt cggctcggca accacggtcg gccggtcatc
      181 cagtgagtct ggcgtccgct cgtcgagcat agaggattac cggctggcgt gcctccagtt
      241 tgatttgagc ggatccattc tcccagcgga tcgccgactc ctcccgtcga tcaggataaa
      301 gcattgcggc cgccacctcc gctcgtttcc agcccgaggg caccggcaca gctaccgtac
      361 gtggagtcac cgaataacac gcaatcttcc cctctcccaa cggacaatac acggcgtcgt
      421 cgtgcaggat ttctctgtcc cccacggcaa tgcggagagt tttcgcaaca ggattgctct
      481 caatcaaaac gtcgccatcg aaatgaatcc gcgtttcatc gccggtgcgc tcgaagtccc
      541 gcaccgaacg gccgcgcaga acgaagaatg gcaccatgtc gagataaaag gcatcgagga
      601 actcggccgg atcggtatct gcacgaagaa tgctgtgggc gcaggcgccg tagaatcgcc
      661 gatggatccg caccgtggca gcatcgccgc gcgcgccggc gccagagaga ccccacaccg
      721 cgctgtttcc gtaaatcaga gggaccagcg ggatggctgt tccgccgaac ggacacggcg
      781 ccggtccggt gagataccag aagcagctga tgtgaccgat catgggatag cgcaacgcct
      841 ccgagctgac gtcgacaccg tgctgggcaa aatcctgcag gaccttgtaa cgtccatcga
      901 tcagattgcg aaccccgctc gccggatgct gcgggtccca gtcattgcga atggaatagt
      961 aggagaggac gtcgacgtgc gtggtgtgcc gcagccgata gcgttcgcac gtgtagcgaa
     1021 tgcgctccgg tccgggcccc tccatatatt ttgcgagacc cagaatgtaa gactgttctc
     1081 cggtccacgc gcggctcttc cacagttgtc catcgggtcg acgcgcgatg atcgcttcgt
     1141 tccacgcggg actgctgcga tacgcgtcgt cgtagttgtc ggagagcgtg atggtggcgt
     1201 tgagcgcttc accctgctcc atgagccgca tcatgccgtc atatcccccg atgcgctcgt
     1261 tgacgacatt caccgctgga tatccggtat ctttcccgcg gaattgccag ccccagagat
     1321 gcaccacctg cggtgagcga tcgatgagag ccgagacgcg acgcacgatt tcgtggcact
     1381 gctcgaaggt agccgaaggc gcggggaaaa gcggttcgtc gcagcggatg ccgtagacat
     1441 atttgtcatc gtaaaaatgc gtcgggatcg caggcagacg gctgcggacc aattgaccgg
     1501 cagcgatcca cgccttggcc ggatcgcctg tgacgggaag gaaatcgagc cggcaggaag
     1561 atttctgatc gaccaggaga ttcggcgtgt tctgattgcc gcagttgagc ggggcgccgt
     1621 gctcaaggtt caggtcgtag caggcgctgc cattaacgcg atggaccttg atcgtgccca
     1681 tcgccgcgcg ccggctgccg gcatctccag tcacagccag cgtggtccca tccatataag
     1741 aagtggtttc ctgcacgcac agggctcgtg ccgtgccggt catgaccacg ggcagcgtgc
     1801 ccagcacttt accccagaat cggttgggcg gaagactgcc aggtttcgcc tgtgccagca
     1861 tggccaggct cccgccctca tcgccatgcg cgagccatgc gccttcgtcc gattcccgca
     1921 cggtcacgag cccgggcagc gcgacgtcaa ccaactgaaa gggcgcgctc tcttcgatcg
     1981 catccatttc aacgaacaaa gtaggcccat cgatcctgta atgcagagtg aaatgcgcag
     2041 cgggactacc cccgaacaga ggcgcaaagc ggaaagacac cgagctatgg gtcgcgtgat
     2101 ggttttcagg cgtcagcggc aaggtctgaa aactccacgg ctccgtgcgg cagagtgtga
     2161 cggcgacagg cgccccgaag gtttcgcccc agagttcggc gttgttggac gataagcgat
     2221 atttccaggg caggccgctc gcggcatcga gcgtcacctc aagttgcgaa ctgctcagaa
     2281 ccagcgggtg ttcgggtgca tggcgctcgc cgtcggcgat ctgttcctgg gcctcaccgt
     2341 tcggcgtcag ccagccggct ccggccgccg cagctccggc tgcgctccac tgaagaaaat
     2401 gacggcggct gaagcgttcg gggtcgcact gcatttctca ctcctcgcgg ggcagctgtt
     2461 cactgcgtgt tgtggattga cgttggcgcg actttgccag gtgccgaaac ctggcaacac
     2521 gcccaatttt ttaacgtgcc gggaaaaagc tgtgccgcgg cgtcgtaacc gcggcacagg
     2581 gaggcatcaa aatgtgatct tcgccccgaa ctgaagctcg cgcggcgtac tggaaagacc
     2641 gttggccagt ccagcggaac tgttgccgat cgtcgtgccc gggcttccga tgatttgagc
     2701 gttgtacaga ttgaacgatt cggcccgtaa ctcgagactg acattctcgt agagcggagt
     2761 ctgcttgacc agggacaagt caagatcgtt gtagaacggg atgcggaatg gcatcttgcc
     2821 catatctccg aacgagccgg aagggatcga gaaggcggat ggattgtatt cgcagaaccc
     2881 agtgggtcca cgcttgccga tcggtgttga gccgcaactg ccatggttcg gatcgccgat
     2941 cacgttggga cgcgaataac tggtcaccga tccgttgtcg ccggagatat tcgccacgtc
     3001 tcccccaacg ttgaggttgt aaggttggcc ggaatgcatc agcaccaggt aattcgtggt
     3061 ccagcctccc agtacgtacg agagtgggcc gctctgtagc cagcgttgct gctttccgaa
     3121 cggcagggcg tacaccgtac tccagctcag cgtattgcgg acgtcatagg aggagattcc
     3181 ataggcgttg cgtggcagaa agaagctctg caccactgac ccgcctccgg tgccgttctc
     3241 cgcactgaac cagccgctgc tgttgtccag agacttgccc catgtgtaag acaggatcgt
     3301 gttcagagag ttcgagaacc tcttctgaaa ctgcgccagc agtgcgttgt aattcgcata
     3361 acctgtatct tccgagtagt gccatcctgg cgcggcccag ggcatggcct tcaggctatc
     3421 gatcgtggta agtggcgtgc cggccggcga cgcatgctgc gcggcattag cgaacccggt
     3481 gaagtccaga cgcgtgctct tcgatcccgc gtatccaatc gtgagcagcg ttgttggcgc
     3541 cagttgctgc tgcacctgca ggttatattc cacagcgcgc tggtccttat agccgggctc
     3601 gttcacgtaa cctccgccgg tcgtcagcca cgggctcgat gcaatgacgg gattggggaa
     3661 attcccctcg agactggtga tttgcgtcag cggattcccc ggacctcctt cccaaactcc
     3721 tccctgggag aagttaacct gctggctcga gatgccggtg gtccagggcc aggtcgggcc
     3781 ttcaatcgtg ttttgcaccc actggctgcg gcccgtgatg gtgtcgaaga agatcccggc
     3841 gccgccattc accacggtac cgttgcgcac ctgccacgcg aatccgagac gcggcccaaa
     3901 attgtccttg atgggcggcc cgacctgttc ggcgccgttc gcaaagacga tgtggtcgct
     3961 gtagggaacg ctggcgagcc cgcccggaat gcacgggttc acaaaagtcg atccgcaggc
     4021 ggcgacattg cgttgaataa tatatttctg gttgaaaata tccagcccat tcgccagccg
     4081 attgttgagc atatggatcg ccggcagata gtcgtagcgc agaccataat tcacagtcag
     4141 cttcggattg acccgccatg aatcctgcac ataacccgac caaagactca tgctgaagta
     4201 gtcctcggag aaatcgggct gctgcgccgt gaacgtagcc ggcagtccca gcaaagccga
     4261 agccagcgag ttcccggttt tggatgcgtt cacattgctc gtggtggagt cgctgaaggt
     4321 gaactgctgg tagagattgc gctgcagccg attctggtag agatagtcca cgcccgcctt
     4381 gatggtgtga gctcccttga tccaggtaac actgccgccg gcgctgacca ccggattgcc
     4441 gcgatcgcgc gtgccatcgt tcccggcatt cgacgtgtca tacggtccgg ccagattgat
     4501 atacataccg ctgtactggc tcaggttggt aaagcctgcc gagctggctg cggcgtagcc
     4561 gcccggcgcc gcggcctgcc ggaacttgta gggcttcagc atcgcgcccc cacgtacatc
     4621 cagaatcagg ccgggcgaga aaacatgcgt gtaaccgccg ccgaaattgt aggcgtgata
     4681 atgcgagaca tttgcatcca ccgttccatt cacgggcgat gtgtcgtagg cccacatctg
     4741 gctgatgcgg ccaaagccga aattcttgtc attcttatga aaatcgaccc tgacctgata
     4801 actatcggtg ttatcgatat tcgagcgatt ctcgatgtag ttatatccgg aagcttcatt
     4861 ggcctgcgcg ttgggaggca ggtagtaggc tttcaggtag ctggccatcg tcccgttaat
     4921 cagccccgca gggatcttca aacagggcgt cccgcccgtt tgcgtgccgt cgctgttcgg
     4981 agtaatcggg tttcccgata catcgcagag gaagggctgg accgtgcatt tgctgccggt
     5041 gcacgcggtc gaccatggat tgtagatggg gtgctggtaa tacgaatacg agctgttgga
     5101 gaagtctccg ctcagctcct gcgacgtcgg aagaagctgt tgcgagagcc ccggtttgct
     5161 gtaacgccat ccctggtagg cgccgtagaa aaacatccgg ttcggccaca tcggcccgcc
     5221 cagcgcacct ccgaattcgt tctgcacata gtgtccaggg ggggcaggtg tcgtgcttga
     5281 ggctcccggg gcgcaccttc caaccgaaca aaagtcgctg aacgtattgc gggcgtcgaa
     5341 aaggttgttg cgatcgaact cccataccga gccatggaag gagttcgtcc ccgatttgga
     5401 gagcatgttc accacacctc cggtgaccac gccgtactga gcatcgtcga tatgagacgc
     5461 gaccttgaac tcctgcatgg catcgatgat gggtaggaac ccgtagatcg cgccccgcag
     5521 gtcggtattc acgatgccat ccatcaggta atacgtctcg cggttctgtt gcccgaagaa
     5581 cgacggcttg tagaagttag ttcccggaat tgcggtaatg ccggcgtcgg tcgtgctgat
     5641 tcccgatccc tgcgccgtcg aaaccggatt cacaccgggc gagaggatga gcagcgaagt
     5701 gaagttcagt ccgttcaaag gaagctgctg gatttccttt gtctcaatga cgctccccaa
     5761 ctcggacgac gacttctcca gcatcacgtc ttccgtcgag gcatttacgt tgaccgtctc
     5821 ctgggcagag cccaccgtca gcgtttcgtt ttcggtcagg atttggccta ccaccagcgg
     5881 gaacggtggc agggcgacct tcttgaatcc ctgcatcgcg accgtcatcg tgtattgccc
     5941 cggcgtgaca tccacgaagg cgaaatatcc gctgctgttg gccgttgtgt gccgcgatac
     6001 cccggtgccc acgctcgtga gcgtcacatt cgcacccggc acgatgctcc ccgtcgaatc
     6061 gctgatggtt ccgtgaacct cgcccgtcga ctgctgagcc tgaagagacg ccgcgaacag
     6121 cgcgagtccc accagcccac acaccattgc aagtatccgg atgcgcatcc gctcctcctt
     6181 aagctgtaac cgctttcttt ttgaatggcc ctggggggca gctccgcttt gatcgggagc
     6241 aacacggagg ttaggttctg ccaggaagac ctgtcaatca aaaatgaaat gatcgtccca
     6301 atatgtgaga cgacaacagc ggccggttca cgcgcacccg cgcccgttca atcgcccgaa
     6361 aggccgtagg gcggctccat ctgcgcagag atcaatggga atggctgctg gttttagctg
     6421 gactctttgt acccaagaga ggccgagatt cggctccccg cccgctgcac atgttgtgcg
     6481 atcgctggga tgcgggcccg cgccatgcgc gagaccggcc ccgacacgct cactgcggca
     6541 gccacgtatc cggaacagtc caggatggga gcggcgatgc agcgcgcccc ctccacgacc
     6601 tcctcgttgt cgatggcgta gccacggctc tggatccgcc ccagttccgc gatcagctcc
     6661 acgggacggc tgatactgcg cggggtgagt ttttcgaatc tcgtcgcggc catcagttca
     6721 tcgcgcaccg ccgacggctg gccggcgagg atcacttttc ccagcgccgt gcagtacagc
     6781 ggtcgccgca ggccggcttt cgatacaagc cggaaactgt gcgggctctc cagcacggaa
     6841 agataaagaa cctctttccc gtcgagcact cccagattga ctgtctctcc ggtttgacgc
     6901 cacagctctt ccaggatggg ctgactcact ctgcagatgg tgccctgata ggtgctcccg
     6961 gcgcccaaat gtaccaggcg tggtccaagc agataggcgc ccgccccatc ccggaccaca
     7021 taccccgcac cttccagatg ggagagaaac cgccacgccg tgctcttgtt cagctttgtg
     7081 agctgtgcaa tggagcgaag ctgcagtccg cccggcgttc gatccagaag atcgaagatg
     7141 gtcagtacct tagtgagaac cccaaccggg gccgtgcctg acttccgtgg tttcattgtg
     7201 aagacaaatt gtggagtctc aatttcatgc taccgctcgg tacaagcagg tgttcccggg
     7261 gcgaatgcca tttcagtgta cgttcggatg agcggagggc ggccgttctc ctgagttcgt
     7321 agcctggatc gttttcgata ttgagatatt acattttgct ccgcttggcg cgatatctgg
     7381 ttttcgagcg tccatcatcc tttaactgtt gccgatgacg gagaggctcc tcgatcgaat
     7441 gcaacctgcc gcgtggcgcg agtcaggtcg ccgccgtcga ggatgatccc accgctctgt
     7501 gcgccctcta ccagcagaaa agttcccgtt ttcacgagaa gccgcggtgc ggtcatcagg
     7561 accgactgcg tgtccgcgaa ccggatggcg gattcggcgt cgggatcggc cgtgaccgca
     7621 agacccgcga tcgagacatc ctccacgcga tccaggatca atgcaggacg gagatccggc
     7681 gtctgcgtct caaatcggat attgttcagc gtcagcgccc gcgcattgcg ggcatacagt
     7741 ccataggcag gcataggccc gagcatgaaa tattcgccgg cgatctgcgc caggtcgcgg
     7801 cgcgctgtat cttccaccgt acctccgccg ccgaatgtca ggtgaatgtt ctcgaacgaa
     7861 atcttttcca gaacagcatc tcctacgcag ttcagcgtaa tgcaggaatg cagttcacct
     7921 ggccgataag cgcttggaaa cggcatcccg ggcagttgcg ggggattcgt cgtcaccgtc
     7981 ccgtgaatgt tgctgaagga gatattcctc gcgaccgctg gctcgttgtt ttccgccggc
     8041 acggcttcca ccggcgatgg gccgctgcct ggccggcgcg gctctcgggg cccgatgctg
     8101 atgcagatcg gacccgtcac ttcgtccaga accagattcg aaaacgacat gttctcgaag
     8161 cgcgaaccgg gaaccccgtg aaacttgatg gggcatccga agacctggta cagaacacag
     8221 ttggaaacgg tgatgtcccg tgcgacacca ccgccgaagc ggaagcacga ccagcgcgtg
     8281 ctgaaggtcg aattcgtgat cgtcacatac ctgcagctgc caaatagtgc acacgcgtca
     8341 tcctgggaaa gcacggtgca gtcgctcacc gtaacgtact ggcaactgat gaagtggaat
     8401 ccgtcgttgt tcccgttcac acggttgtag atgtagatgc cgttcatgtg cacgcgccgc
     8461 gactggatga cgcgcacgct gtgataggca ctgttcacca ggtcaatgtc gcgtatgcgg
     8521 acgttttcgc agcggtagag gagcagatga taagggcggt cacgcccgcc cagtccgctc
     8581 ggcggagtcg caccgcgcac cgcagcatga aactggatgc cctggccatc gatcgttccg
     8641 ggcccctcga tggtcacgtt ggacgcattc acggcataga ggagcgccca gttaccgtct
     8701 tcaagcgtgg agtcgccgct gagcgggatg gcgtcgaccg cgtggtagtc gtggccgttc
     8761 gcgctgcc
//
LOCUS       DASZYN010000159         3792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_842134, whole genome shotgun sequence.
ACCESSION   DASZYN010000159 DASZYN010000000
VERSION     DASZYN010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842134"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VHE33_04060"
     CDS             <1..519
                     /locus_tag="VHE33_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96656.1"
                     /translation="LILLDEMGRGTSTFDGLALAWATLEFLQRHIGARALFATHYHEL
                     TLLADEFPRLKNLHVEVRETPQGIVFLHRIEQGPANRSYGIDVARLAGLPPAVIQRAR
                     QVLKQHERSERHNVAVETEPSLQLTMFTPLSQRILDRLAETDIDTLTPLKALNLLEEL
                     KAEIGDTKASAR"
     gene            534..1754
                     /gene="nagZ"
                     /locus_tag="VHE33_04065"
     CDS             534..1754
                     /gene="nagZ"
                     /locus_tag="VHE33_04065"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HVT96657.1"
                     /translation="MRQRPTAVTNSGPNAPSPNGRRIARVFMPRRRNVQLGEQAGQIL
                     IAGLGGVSLSAEERAWLERIRPGGIILFRRNIESAAQVASLLRDAGEHSATPLFRCID
                     LEGGLVDRARDLIYPMPSPAAVFATGKRNLYRQHGWLIAREARALGCNAVFAPVLDLA
                     LPESASVLRTRAVSAEPGRVIDYARGFLAGMKNENVLACGKHFPGLGAGRVDSHQSMP
                     VIHRQTRQMWRTDLAPWLALASHLPFAMVAHASYPWPGRDSVPATVSRYWVTNVLRRQ
                     IRFKGIILSDDMEMGGILSQMSIEEAVIQALLAGIQMIEICRDPALIQRAYEAILKEG
                     ERSRAFRDVVASAWRKIYRSKKSWLHPTRRQNLSDTRLNRLCDDVKEFAALVGETPAT
                     SAPDPERWKGLQKE"
     gene            1844..2062
                     /locus_tag="VHE33_04070"
     CDS             1844..2062
                     /locus_tag="VHE33_04070"
                     /inference="COORDINATES: protein motif:HMM:NF040493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96658.1"
                     /translation="MSGRSQHVTIPAEFRFVTEEVFVRRDPRSGDLILSPAPRSWNQI
                     FAALDAAGFPEDFMNERNQGEPEQRELP"
     gene            2059..2475
                     /locus_tag="VHE33_04075"
     CDS             2059..2475
                     /locus_tag="VHE33_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HVT96659.1"
                     /translation="MTLYLLDTNMVSYILKGKSPAARSRLEKLRIQKQQEAAISTITV
                     SEILYGLERIGAGAQRRRALDLFFASIRICQWDQAAAEAYARLRVRQELLGRPLGPYD
                     MQIASHALALGATLVTHDKAFRHVTGLAVEDWASDV"
     gene            complement(2552..2980)
                     /gene="rnpB"
                     /locus_tag="VHE33_04080"
     ncRNA           complement(2552..2980)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VHE33_04080"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(3138..3611)
                     /gene="moaC"
                     /locus_tag="VHE33_04085"
     CDS             complement(3138..3611)
                     /gene="moaC"
                     /locus_tag="VHE33_04085"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006870.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HVT96660.1"
                     /translation="MSKLSHFDDAGEARMVDVSAKAVTRREAEASVFVALNAETLAAL
                     PNNPKGNPLEVARIAGIQAAKRTSELIPMCHPLPLSFVDVRTEVVAEGIAIRATAATT
                     AGTGVEMEAMTAAAVAALTVYDMCKALDKGIVIRDLRLERKTGGKSGEYTRANKQ"
BASE COUNT          676 a         1267 c         1079 g          770 t
ORIGIN      
        1 ctgattttgc tcgatgaaat gggtcgcggg acctcgacct tcgacggcct cgcgcttgcc
       61 tgggccacgc tggagttcct ccagcgccat attggcgccc gcgccctttt tgccacccac
      121 tatcacgagc tcaccctgct cgccgacgag tttcccagat taaaaaacct gcatgtcgaa
      181 gtcagagaaa cccctcaggg catcgttttt ttgcatcgga tagaacaggg gcctgcgaat
      241 cggagttacg gaatcgacgt cgcgcgcctg gccggattgc ccccggccgt tatacagcgc
      301 gcccgtcagg tgttgaagca gcacgagcgc tcggagcggc ataatgttgc cgtagagacc
      361 gagccttcgc tgcaactcac catgttcact ccgctttcgc agcgcatcct cgatcgtctg
      421 gcggagaccg acatcgatac gcttacgcct ttgaaggcgc tgaatcttct ggaagaactg
      481 aaagccgaaa tcggggatac gaaagctagc gcacgctgaa gcgtaccgac ccaatgaggc
      541 aacggcccac agcagttaca aattccggcc ccaatgcgcc ctcccccaat ggccgccgca
      601 tcgcccgtgt ctttatgcca cgacgacgca acgttcaact cggcgagcag gctggccaga
      661 tcctcatcgc tggtctgggc ggcgtctcgc tctccgccga agagcgtgcc tggctggagc
      721 gcatccgtcc cggcggcatc atcctcttcc gccgcaatat cgaaagcgcc gcccaggtcg
      781 cctcgctgct gcgtgatgcc ggcgaacaca gcgccacgcc gctcttccgc tgcatcgatc
      841 tcgagggcgg actcgtcgac cgcgcccgcg atctcatcta ccccatgccc tcgcccgccg
      901 ccgtcttcgc aaccggcaag cgcaatcttt atcgccagca cggctggctc atcgcccgcg
      961 aagcgcgcgc tttgggctgc aacgccgtct tcgctcccgt cctcgacctc gctctgcctg
     1021 agtccgcctc ggttctgcgc acccgcgccg tctccgccga acctggccgc gttattgatt
     1081 acgctcgcgg cttcctggcc ggcatgaaga acgaaaatgt cctcgcctgt ggcaagcatt
     1141 ttcctggact cggcgccggc cgggtcgact cgcaccagtc gatgcccgtc atccatcgcc
     1201 agacgcgcca gatgtggcgc accgacctcg ccccgtggct ggccctcgcg tctcatttgc
     1261 cctttgctat ggtcgcccac gcgtcgtatc cgtggcccgg ccgcgattcc gtccccgcca
     1321 ctgtctcccg ctactgggtc accaacgtcc tccgccgcca gatccgcttc aaaggcatca
     1381 ttctctccga cgacatggag atggggggca tcctctcgca gatgtccatc gaggaagccg
     1441 tcatccaggc gctgctcgcc ggcatccaga tgatcgaaat ctgtcgcgat cccgcgctca
     1501 tccagcgggc ttacgaagcc atcctgaaag aaggcgagcg ctcccgcgcc ttccgtgacg
     1561 tggtcgccag cgcgtggcgc aaaatctacc gctccaagaa aagctggctc catcccaccc
     1621 gccgccagaa cctttccgac acccgtctca accggctctg cgacgacgtg aaggagttcg
     1681 ctgcccttgt cggtgagact ccggcgactt cggcgcccga tcctgagcgc tggaagggtc
     1741 tgcagaaaga atgaagatca ggcttgccgg gatgcctgcc agatagctat gctgtatagc
     1801 aggggttgaa acgatgccaa aactcgccaa agcccgagtg ttcatgagcg gccgaagtca
     1861 gcacgtcacc atcccagccg agtttcgttt cgtcaccgag gaagtcttcg tacgccgcga
     1921 tcctcgcagt ggcgacctga ttctttctcc cgcgccccgt agctggaacc agattttcgc
     1981 agccctcgat gccgctggat tccccgagga ctttatgaat gagcggaatc agggtgaacc
     2041 agagcagcgc gagttaccgt gacactctac ctgctcgata ccaatatggt cagttacata
     2101 ctcaagggga agtcgccggc agctcgcagc cgccttgaga aacttcgtat ccaaaaacag
     2161 caggaagccg caatctccac gatcacggtg agtgaaattc tttatggcct ggaacgtatt
     2221 ggtgccggag cccagcgccg ccgagctctc gacctgttct ttgccagcat caggatttgc
     2281 cagtgggatc aggccgccgc cgaagcttat gcgcggcttc gagttcgcca ggaactgctc
     2341 ggcaggccgc tgggtcccta tgatatgcaa attgcctcgc acgctcttgc cctgggagca
     2401 actcttgtaa cgcacgacaa ggccttccgc cacgtcactg gcctggcagt tgaagattgg
     2461 gcctctgacg tctgaacgca ctctgcgatt tcgaaacgat ggaaccaatt tgaaggcccc
     2521 ggctctcctt atcggaaaac cggggccgtg atcaacaagc agacctgtaa gccggattct
     2581 gtcgaggacg gccattcctc tgggcgacgc attactgcgg cgctcgtgta tagcgaccta
     2641 cccggaactt tcatcccttg caggatgccc cgttcggggc tgccgcgccg ggccggcgcg
     2701 tgccggcatt gctgccggac tcgttcccta tttggtcttg ctccgtgtgg ggtttaccct
     2761 gcctccgtcg ttaccgccgg agcggtgcgc tcttaccgca ccttttcacc cttaccctga
     2821 cctcttgcga ggtgcgggcg gtttgttttc tgtggcactg gccgtctctg agctttgaca
     2881 ctcagatccc ggatgttatc cggcacactg ccctgaggag tccggacttt cctctgtccg
     2941 atcgcttgcg ctttcgaaca gcgaccgccc agtctgcctg ctgctctatc ttaccgcggg
     3001 ttgggtggga accggaatca gactggactg cacgtccggg gtaatcgaac cgagccgggc
     3061 accggttcca gcgcgacccg gcagagttct tcggatgccc agaatcggat gggttggctg
     3121 agccgcggag aaagtgttca ttgtttgttc gcgcgtgtgt actcgccgct cttgccgccg
     3181 gtttttcgct ccagacgcag atcgcgaatc acgatgccct tatcgagcgc cttgcacatg
     3241 tcatagacgg tgagcgcggc gaccgcggcg gcggtcatcg cttccatctc gacgccggta
     3301 ccggccgtgg tggcggcggt ggcgcgaatc gcaatgccct ctgcgacaac ctctgtgcgg
     3361 acatcgacaa agctgagtgg caaagggtgg cacatgggaa tcagttcgga cgtgcgcttg
     3421 gcggcctgga tccccgcaat gcgcgccact tccagcggat tccctttcgg attgttcggc
     3481 agcgcggcga gtgtctcggc gttcagcgcc acgaacacgg aagcttcggc ctcccggcgc
     3541 gtgaccgctt tggcgctgac atcgaccatg cgcgcttccc cggcatcatc aaaatgcgaa
     3601 agcttgctca tgctgggaaa gaggatacac ggtgcgccat cgcccgtctc cagctcgccg
     3661 ttagctcgtt tcgcgctcgt tttccgcgcg cagctgacgc tccgcggtcc acggacaatg
     3721 gcggcagccg gagccgcagc aataaccgcg acgcaggtgg tacgcctcgg tgaagacaac
     3781 gtacggacct tc
//
LOCUS       DASZYN010000160         4154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_263464, whole genome shotgun sequence.
ACCESSION   DASZYN010000160 DASZYN010000000
VERSION     DASZYN010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263464"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..603)
                     /locus_tag="VHE33_04090"
     CDS             complement(121..603)
                     /locus_tag="VHE33_04090"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HVT96661.1"
                     /translation="MNALREFYLERRRVELPVFLKVLRALPADKMEYKPHEQSPSAEQ
                     LVWTLTGELKTCVTVAREHRGEWKSDPAPPLAEMIAIFERTANELVEAVAAMDDAAWD
                     APAKFYYGGKMVSEQPASGFLWMIHFDAIHHRGQLTTYLRPMGSKVPAIYGPSGDERS
                     "
     gene            complement(617..1810)
                     /gene="galK"
                     /locus_tag="VHE33_04095"
     CDS             complement(617..1810)
                     /gene="galK"
                     /locus_tag="VHE33_04095"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HVT96662.1"
                     /translation="MGGCSGKCSRERACMLDVNGIRAKHAKRFDAEAAVFLAPGRVNL
                     IGEHTDYAGGFVMPAAIDFGTLAAISKRDDGQVVIRSENFDEQVSHPLDGFPQRGGGH
                     WSGYPLGVVKVLREAGFAVPGFSLTIEGDVPVAAGLSSSASIEVATAFAALSLARANP
                     AREEIAKLCQRAENTYVGSSCGIMDQFIACRGEENHALLLDCRSLEYRLAPIPPSVSL
                     VIANTMVKHSVAGGEYGNRRAEVEEGTAILRGHRPEIQLLRDATPQDLEQWGAEMPDN
                     VLRRCRHIITENLRTVAAADALAAGQMRKLGDLMAAAHASYRDDFEASCEEADIMVEA
                     AQHLPGLIGARLTGGGFGGCTVNLVHADHAEQFAEGLHEEYRARTGIDAEIYRCHASA
                     GAHAV"
     gene            complement(1870..3213)
                     /locus_tag="VHE33_04100"
     CDS             complement(1870..3213)
                     /locus_tag="VHE33_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVT96663.1"
                     /translation="MQRSLTHDAELLAEAVDRLEGEFAGLPSFEPEVPGEGRMAAVLA
                     EAAQRLGDNFPYFHPLYAGQMLKPPHPVARAAYALAMRLNPNNHALDGGRASSRMEVE
                     AVGEIAAMFGWTRFLGHLTSGGTFANLEALWVAGLMAPGKKVVASEQAHYTHSRISGV
                     LKLPFTSVETDDRGRMDVAGLEGLLKEGDIGTVVATLGTTAVGAVDPLDRILALRERY
                     GFRVHVDAAYGGYFRLASNLGCEAETAFEGIGEADSIVVDPHKHGLQPYGCGCVLFRD
                     PAMGRWYKHDSPYTYFTSAELHLGEISLECSRAGASAVALWATQRLLPLVRAGEFAHG
                     LEKGREAALQMHRRIAADPRFVAGPMPELDIVTWAVRAPTREESSRRAQEIFDRAAEQ
                     NLHLALAHLPARFFQGSEWPDGDSEATVTCLRSVLMKPEHLSWVEEIGKRLEKAV"
     gene            3405..3794
                     /locus_tag="VHE33_04105"
     CDS             3405..3794
                     /locus_tag="VHE33_04105"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HVT96664.1"
                     /translation="MDWYLMVWQKYAQFTGRSRRREYWMFALFNMIFCCVLYAAILVA
                     FVADQKTIGIFLACLSGIYVLAALIPGLAVSVRRLHDINKSGLWLLLNFIPFGGLVLL
                     ILACIESDPGTNQYGPNPKLFPQAAMS"
BASE COUNT          767 a         1590 c         1028 g          769 t
ORIGIN      
        1 gcaatctctg cgtcagccac ctagctcaga ttcggcttcc tgcgattctt acattgccgt
       61 tccggcaagc ggtatgaccc ttctgtggaa tcctttccat tccttcaaaa ttcgcagtcc
      121 tcaggaacgc tcgtccccgg acggcccata gattgccggc accttgctcc ccatcgggcg
      181 caaatacgtc gtcagctgcc cgcgatgatg gatcgcgtcg aagtggatca tccacaggaa
      241 ccccgatgcc ggctgctcgg agaccatctt tcccccgtaa tagaacttcg ccggtgcgtc
      301 ccaggccgca tcgtccatcg cggcaaccgc ttccaccagc tcgttggcgg tgcgttcgaa
      361 aatcgcgatc atctctgcca gcggcggcgc cggatccgac ttccactccc cgcgatgctc
      421 ccgcgcgacc gtcacgcagg tcttcagttc cccggtcagc gtccaaacca gctgttcggc
      481 ggaaggcgac tgctcatgag gcttgtactc catcttgtcg gccggcagag cccgcagcac
      541 cttcaggaac accggcaact ccacccgccg ccgctccaga taaaactccc gcaaagcatt
      601 catgcagtcc tcctcgtcac acggcgtgcg cccccgccga ggcatggcag cgatagatct
      661 ccgcatcgat ccccgtgcgc gcccgatact cctcgtgcag cccctccgcg aattgttccg
      721 cgtggtccgc atgcaccagg ttcaccgtgc atcctccgaa gccgccaccg gtcaggcgcg
      781 ctccaatcag ccccggtaga tgctgcgccg cctccaccat gatgtccgcc tcctcgcagc
      841 tcgcctcaaa atcgtcgcga tagctcgcgt gcgccgcggc catcaggtca cccagtttcc
      901 tcatctggcc cgccgccagc gcatcggctg ccgccaccgt ccgcagattt tccgtgatga
      961 tgtgccggca ccgccgcaga acattgtccg gcatctccgc tccccactgt tccagatcct
     1021 gcggcgtcgc gtcgcgcaac agctgaatct ccggccgatg cccgcgcagg atcgccgtgc
     1081 cctcctctac ctcagccctg cgattgccgt attccccgcc cgccacgctg tgcttcacca
     1141 tcgtgttcgc aatcaccagg ctcaccgacg gcggaatcgg cgccagccgg tactccagcg
     1201 accggcagtc cagcagcagc gcgtgattct cttctccccg gcatgcgatg aactgatcca
     1261 tgatcccgca cgaggacccg acataagtgt tctccgcgcg ctggcacagt ttggcaatct
     1321 cctcccgcgc cggatttgcc cgcgccaggc tcagcgctgc aaaggccgtg gccacctcaa
     1381 tcgacgccga cgaactcagc ccggctgcga ccggcacatc gccctcaatc gtcagactga
     1441 accccggtac ggcgaatcca gcttcgcgca gcaccttcac cacgcccagc ggatagcccg
     1501 accagtgccc gccccccctc tgcggaaaac cgtccagcgg atggctcacc tgctcgtcga
     1561 aattctccga ccggatcacg acctgcccat cgtcgcgctt gctgatcgcc gcaagggtgc
     1621 caaaatcgat cgccgccggc atcacgaacc cgcccgcata gtccgtgtgc tcgccgatca
     1681 ggttcacacg cccaggtgcc agaaacactg ccgcctcggc gtcaaatcgc ttcgcgtgtt
     1741 ttgctctaat accgtttaca tccagcatgc aggccctttc tctgctgcat ttcccactgc
     1801 aaccacccat agcctgtccc taaatgtact gaattcgttc ttctgcgcag cccgcccagc
     1861 caccagccgt tataccgctt tctcgagcct cttcccgatt tcctctaccc agctaaggtg
     1921 ctccggcttc atcagaaccg accgcagaca cgtcaccgtc gcttccgaat ctccgtccgg
     1981 ccattccgag ccctggaaaa accgcgccgg cagatgcgcc agcgccagat gcagattctg
     2041 ttccgccgcc cgatcgaaaa tctcctgcgc ccgtcgcgac gactcctccc gcgtcggtgc
     2101 ccgcaccgcc cacgtcacaa tatccagctc cggcatcggc ccggcgacga aacgcggatc
     2161 ggcagcgatc ctgcgatgca tctgcagcgc cgcctcccgc cccttctcca gcccatgggc
     2221 gaattctccg gcgcgcacca gcggcagcag ccgctgcgtc gcccacagcg ccaccgccga
     2281 cgctcccgcc cgcgagcatt ccagactaat ttcccctaaa tgcagctcgg ccgacgtgaa
     2341 ataggtatac ggcgagtcgt gcttgtacca ccgccccatc gccggatcgc ggaacagcac
     2401 acacccgcac ccgtacggct gcaggccatg cttgtgcgga tccaccacga tcgagtctgc
     2461 ctcccctatg ccctcaaacg ccgtttcggc ctcacaccca agattcgacg ccagccggaa
     2521 atacccgccg tacgccgcat ccacatgcac ccggaaaccg tatctctccc gcagcgccag
     2581 aatccgatcc agcggatcca ccgcccccac cgccgtcgtg cccagcgtcg ccaccaccgt
     2641 cccgatatcg ccctccttca gcaatccttc gagccctgcc acatccatcc ggccacgatc
     2701 gtcagtttca acagacgtaa acggcagctt taagactccg ctgatcctgc tgtgtgtgta
     2761 gtgcgcctgc tcggaagcca caaccttctt ccccggcgcc atcaagcccg ccacccacag
     2821 cgcctccaga ttggcgaacg tccccccgct cgtcaggtgg cccaggaacc gggtccatcc
     2881 gaacatcgcg gcaatctcgc cgaccgcctc gacctccatc cgcgagctgg cccgtccacc
     2941 atccagcgcg tgattgttcg gattcagccg catcgccagc gcatacgccg cgcgcgctac
     3001 cggatgcggc ggcttcagca tctgccccgc ataaagggga tgaaagtagg ggaaattgtc
     3061 gcccagccgc tgcgctgcct cggccagcac agccgccatc cgcccttcgc ccggcacctc
     3121 cggctcgaaa ctcggcagcc cggcaaactc gccctccagc cgatccacgg cctccgccag
     3181 cagttccgca tcgtgcgtca ggctccgttg catagacgga tcagaatata cctgcttcgt
     3241 tcaacggctg ttgtcccgga tctcgctgtt cgaagctgac tcggatcgtg ctatgcttcc
     3301 gccgactcgg catcgcctgc cgcccacgcg ctccagcttc cgcgatgccc acgaccatcc
     3361 cccgtcaaaa tcacccaacc ctgacttcgg aacggagaaa agcaatggat tggtacctga
     3421 tggtctggca aaaatacgcg cagttcaccg gccgctcccg ccgccgggag tactggatgt
     3481 tcgccctgtt caacatgatc ttctgctgcg ttctctacgc agccatcctg gtcgccttcg
     3541 tcgccgatca gaagaccatc ggcatctttc tcgcctgcct gtccggaatc tacgtcctgg
     3601 ccgctctcat ccccggcctc gcggtcagcg tccgccggct ccacgacatc aataagagcg
     3661 gactatggct cctcctcaac ttcatcccct tcggcggcct cgtcctgctg atcctggcct
     3721 gcatcgaaag cgacccggga accaaccagt acggccccaa tccgaagcta ttcccgcagg
     3781 cggccatgag ttgatccccc gccccggaaa gcccttccac gcgggcgttt cctcgggtca
     3841 aaaccaggaa tctgatacac ttggcccagc gttagaaccg aaccgggcaa gtgcagtctt
     3901 ccgcctcgcc caacaaagca ggatcagcag gtctgccagt tcgctccgat tcactccagc
     3961 gcaattcaat tttcaggaca cagcaacaca atggaacaag gaacagtgaa gtggtttaac
     4021 gacgcgaagg gctacggctt tatctcgcgc cagaacggcg aggacgtctt cgtgcactac
     4081 tctgccattt ccgcaaatgg cttcaagagt ctgcaggaag gtcaggccgt acagttcaac
     4141 gtcgtgaagg ggcc
//
LOCUS       DASZYN010000161         6544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1227591, whole genome shotgun sequence.
ACCESSION   DASZYN010000161 DASZYN010000000
VERSION     DASZYN010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227591"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..736
                     /locus_tag="VHE33_04110"
     CDS             356..736
                     /locus_tag="VHE33_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96665.1"
                     /translation="MRRTSARLLYWSPRFLSILFALFLSVFALDVFGEVHGAGPIALA
                     LFVHLIPAIMVIAVLIIAWRWEWIGALAFALAALGYAVSVLPHHLDWVLTISLPLIVI
                     ATLFLTNWMERTQLRVLREPHTGH"
     gene            complement(763..1623)
                     /locus_tag="VHE33_04115"
     CDS             complement(763..1623)
                     /locus_tag="VHE33_04115"
                     /inference="COORDINATES: protein motif:HMM:NF017006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0050113 - inositol oxygenase activity
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol oxygenase family protein"
                     /protein_id="HVT96666.1"
                     /translation="MSTVAPQTNAPLRDLEQWDEFLEGRYREGKSKEEFRQYDAQANP
                     GVAEFYRLNHENQTVDYVLGKEKQYFGLNKGKKTVWEAAEFLNTLVDDSDPDTDLTQI
                     EHLLQTSEAIRADGHPRWMVLTGFIHDLGKCLCLYGEPQWGVVGDTFPVGCAWSQDIV
                     FPEFFAKNPDRNVAEYQTKYGIYEPNCGLENVHMSFGHDGYIAEVMKPYLQDEALYML
                     RFHSFYPWHKHGAYDHLCNDKDRAMLEWVLKFNQYDLYSKGHAKPNLKELKPYYDDLF
                     AEFLPPVLSW"
     gene            complement(1901..5377)
                     /locus_tag="VHE33_04120"
     CDS             complement(1901..5377)
                     /locus_tag="VHE33_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT96667.1"
                     /translation="MKMIRLWSLIAVFCAMSIAASAQLSTATAFGTVMDSSGAAIPNA
                     TVVFTQTQTNFTRTTTTDGQGQYHAEFLPVGPYSVKVNAGGFQQLVQNGIVLAGTQQA
                     AINFTLHPGVQTSVVQVTAEVPLVNTGDSVLGATIDNRGIENLPLVSRNVYDLLNLTP
                     GVQSVSNENSIGLPMEHVIINGSTDNMVGQVTYYLDGGINMTGVRDTGNVIPNPDAID
                     QFNVQTNNFSAEYGRTGAGVVSVLTKSGTNEVHGSVFEFHRETNFNANSYLQDTRTPE
                     HINRFGATLGGPAWKNKIFLFGSYGGLRQISPHNFNTVVPDALQRAGNFSENLPSTTP
                     TAGLGACATKLSNADKSSTDYGGKFFVCDPVTHQPVPGNRLDLDPNFAPDPVAAAVLA
                     KNVPLPSANRPSPDNRFVGNIGLPNNTDEYLIKGDVQLIPEHRTSLEYYQSIGSQIIL
                     PSGGNVPGWALSNYAYRQQNANASDVWTLSPRSVNQIWLSYSRMMAGRISSPATSLAA
                     YGSDLNVQGTPSLPNISVSNFFTLGQAITGPLAGDNVYGIRDVFSTTKGRHTINTGGE
                     LYLEKDRLQTLLNNYGAFSFTSAAVPTTASGQATYTRTGVAMADFLIGHPNTMSQDSP
                     DDANENYWNYGAFVQDDWRLFPSLTVNLGLRYDVQTAPTDTQRRIAVFEPGVQSTVSP
                     NAMLGQLFPGDPGVPDGGVDTNYNHFSPRAGFAWDIFGNGRTVLHGASGLFFDTIGGN
                     EWMLSQNFQPFAVRETNAFSHVVSLQHIYSTDCQDFAGCVSPFPYLYDKANPRYVSPA
                     SLVFVQKGMRWPYNIQANFGIQQQLTKDLAVSINYVGEFTRKLPLYIDRNAPIYDTTN
                     PSANTTGDVNCRRPFDAIPFKTANTCASPAPGSKYMSNGYVIEDGQSANYHGLQIDVQ
                     KRLSHGLSLHGFYIWSKALSSASMQTTGNIGNSGTTEPEDYYNLPLERQREANDMRHQ
                     AVIYGVWKPNYFEHANLLARTILDGWSLAADLSMNSGQPFSITSGNDDNLDGDNNDRP
                     NVVPGMRPHVLDTSRSRVAERAEWFDTSAFCRVGTSGCSAGGGPSGLDGLVSPTPLDG
                     PGYKNVNASLFRDFSIEGRVKFQFRGEATNVFNFVNLNNPGGTLSSTSSFGVISGAAS
                     MRVLQVGGRLLF"
     gene            complement(5496..>6544)
                     /locus_tag="VHE33_04125"
     CDS             complement(5496..>6544)
                     /locus_tag="VHE33_04125"
                     /inference="COORDINATES: protein motif:HMM:NF027673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HVT96668.1"
                     /translation="VYSPSIWAGWYSGRYTEYESALEKARGTVPHFMHIEWGADSHAG
                     RHAEDPDQALAAVRTGRGTAEVGFAYKRSGGPVRVSSDGDWSETYACDLFDWYLKTLA
                     KLPWMTGAAQWCFKDFTTALRDDNPIPRVNQKGLVARDLTPKESYYLFQSWWAEKPMV
                     HIYGHSWPVRWGKPGQQREVRVYSNCRVVELYLNGKPVGVRQRDPDNFPAAGLRWSLA
                     FREGENELRAVARTDDGSIEDSIGFTYETRTWGSPARLTASLGDRGSEATTIDAALVD
                     SAGVRCLDSRAVVCFSLAGNGRLQDNLGTTTGSRVVEMCNGRAQIRVSHTDPVIVGVH
                     SAGVEPAFVQIPSG"
BASE COUNT         1298 a         2113 c         1760 g         1373 t
ORIGIN      
        1 cctcgaaggc gacgtcgtct gcgccggcaa tgtctacagc ttccagcagc cggtcaacgt
       61 caaagatgtt cagaaagccg tcaacgtccc cagccccgac ccgcccgcca aatggatgtc
      121 cgagcacagc acctgcgccc cgttatcccc caactagggc cttgtccgca agtgcttcag
      181 atagaaaacg tgtcatttcg agagaaaggg gccccaaacg ccatttcagt ttgggggtgg
      241 tgaaccgaga aacctgcttt tcgcgctcct gatggaaacc ctcttgacgc cgcgcgccgg
      301 cggctttcca atcatcccga gctcatgcgc tttccccagc aaggaggtga aacgtatgcg
      361 ccgtacttcc gcccgcctgc tttactggtc tccccgattc ctctccatcc tgttcgccct
      421 cttcttaagc gtgtttgcgc tggatgtctt cggtgaagtt catggagccg ggccgatagc
      481 gctcgccctc ttcgtccatc tcattcccgc gatcatggtc atcgcggttc tcatcatcgc
      541 ctggcgctgg gaatggatcg gggctctcgc gtttgcgctg gccgccctgg gctatgccgt
      601 cagcgtgttg ccgcaccacc tcgactgggt gctgaccatc tcgcttccgc tgatcgtcat
      661 cgccacactg ttcctgacga actggatgga acggacacaa ctccgcgtcc tgcgggagcc
      721 gcacaccgga cactaagcct gagcgatctt ccggtctctt ccctaccagc tcaaaaccgg
      781 cggcaaaaac tccgcaaaca gatcgtcgta atacggcttc agttccttca gattcggctt
      841 cgcgtgcccc ttggagtaaa gatcgtactg attgaacttc agcacccact ccagcatcgc
      901 ccggtccttg tcattgcaca gatggtcgta cgcgccgtgc ttgtgccagg gatagaacga
      961 gtggaatcgc agcatataaa gcgcctcgtc ctgcagatac ggcttcatca cctcggcgat
     1021 gtacccgtca tgcccgaacg acatgtgcac gttttccagc ccgcaattcg gttcgtagat
     1081 cccgtacttc gtctggtatt ccgcaacatt ccgatccgga ttcttcgcga aaaactccgg
     1141 aaacacaatg tcctgcgacc acgcgcatcc caccgggaac gtatctccca ccacgcccca
     1201 ctgcggctcg ccatacaggc acaggcattt gcccagatcg tgaataaacc ccgtcagcac
     1261 catccaccgc ggatgcccat ccgctcgaat cgcctccgac gtttgcagca gatgctcgat
     1321 ctgcgtcaga tccgtgtccg ggtcgctgtc gtccaccagc gtgttcagga actccgccgc
     1381 ctcccaaacc gtcttctttc ccttattcag gccgaagtac tgcttctctt tccccagcac
     1441 ataatcgacg gtctggttct cgtggttcag ccgatagaac tcggcgacgc ccggattggc
     1501 ctgggcgtcg tactgccgga actcttcctt cgacttcccc tcccggtacc ggccttcgag
     1561 aaattcatcc cattgttcaa gatcgcgaag cggtgcattc gtctggggcg caactgtact
     1621 catctggggt ctcctcgcgc ctccagtgta gcgcgaatgt ccagcccccc ggcatgcctg
     1681 gcagttcccc ttcgctccac cccagcggat tgccccctgg cgccgccgca tacagaaagc
     1741 ggggtcctcc ctttcaacaa ggaagacccc gcaatccact tctacccagg tggatgctgg
     1801 ttctgatcct tcaccttgtc atcccgaccg gcccctgagc gtagcgaagg gacggctctc
     1861 gtccgaatgc caacgatgaa gaaccctcag aacacctcgg ttagaacagc aacctgcctc
     1921 caacctgaag cacgcgcatg ctcgccgctc ccgagatcac cccgaacgaa ctggtgctgc
     1981 tcaacgttcc gccaggattg ttcaggttga cgaagttgaa gacgttggtg gcttctcccc
     2041 ggaactggaa cttcacacgc ccctcgatac tgaagtcccg gaacagagag gcgttcacgt
     2101 tcttgtatcc cggcccgtcg aggggagtcg gactcaccag gccatccagc cccgaaggtc
     2161 cgccgcccgc cgaacaaccc gaagtcccca ctcggcagaa ggccgacgtg tcgaaccact
     2221 ccgctcgctc tgcgacccgc gaccggctcg tgtccagcac atgtggcctc atgcccggca
     2281 caacattcgg ccggtcgttg ttgtctccgt ccagattgtc gtcattcccg ctggtgatgc
     2341 tgaacggctg tccgctgttc atcgagagat ccgcggccag tgaccaacca tcgagaatgg
     2401 tccgagccag caggttcgcg tgttcgaagt agttcggttt ccacacgcca tagatcactg
     2461 cctgatggcg catgtcgttg gcttcgcgct ggcgttccag tggaaggttg taatagtcct
     2521 ccggctccgt cgtcccgctg ttcccgatgt tgcccgtggt ctgcatcgaa gcgctggaaa
     2581 gagctttgct ccagatatag aagccgtgga gactcaggcc gtggctcagt ctcttctgaa
     2641 cgtcaatctg aagtccgtga taattcgccg actgcccgtc ctcgatcacg tacccgttgc
     2701 tcatgtactt cgacccggga gcggggctgg cgcaggtgtt tgcggttttg aaaggaattg
     2761 cgtcgaaagg acgacggcaa ttgacgtctc cggtcgtgtt cgccgacgga ttggtcgtgt
     2821 catagatcgg cgcattgcgg tcgatataga gcggcagctt gcgcgtgaac tcgccaacat
     2881 aattaatgct gacggccaga tccttcgtca gctgttgctg aatgccgaag ttcgcctgga
     2941 tgttatacgg ccagcgcatc cccttctgca caaataccag cgaagccggg gacacatagc
     3001 gaggattcgc cttgtcgtac agatacggga acggggaaac gcaaccggcg aaatcctggc
     3061 aatcggtcga gtaaatgtgc tgcaggctca cgacgtgcga aaacgcgttc gtctcccgca
     3121 cggcaaaggg ctggaagttc tgcgacagca tccattcgtt gccaccgatc gtgtcaaaga
     3181 acaggccgct cgctccatgc agtaccgtgc gcccgttgcc gaagatatcc cacgcaaagc
     3241 cggcgcgcgg tgagaaatgg ttgtagttgg tgtcgacgcc gccatccggc actccaggat
     3301 cgcccgggaa caactgcccc agcatcgcat tgggcgaaac cgtcgactgc acacccggct
     3361 cgaagaccgc gatacgacgc tgcgtatccg tcggcgccgt ctggacgtca tagcgcagac
     3421 ccagattcac agtgagcgac gggaacaggc gccagtcgtc ctgaacaaaa gcgccgtagt
     3481 tccagtaatt ctcgttcgca tcgtccgggg aatcctggct catcgtattc ggatgcccga
     3541 tcaggaaatc ggccatcgcc acgccggtcc gtgtgtaagt cgcctgccca gaggccgtcg
     3601 tcggaactgc cgcgctcgtg aagctgaacg ctccatagtt attcagcagt gtctgcagtc
     3661 gatccttctc cagatacagt tcgccgcccg tattaatggt gtggcgtcct ttcgtcgtgc
     3721 tgaagacatc ccgaataccg taaacgttgt cacccgccag cggacccgtg atggcctggc
     3781 cgagcgtaaa gaagttcgag acgctgatgt tgggcagaga aggagtcccc tgtacgttga
     3841 ggtccgaacc gtacgcagcc agagaagtcg caggactgct gatgcgcccc gccatcatcc
     3901 gcgaatagct cagccagatc tgattgaccg aacggggcga cagcgtccac acgtcgctgg
     3961 cgttcgcatt ttgctggcga tatgcgtagt tggacaaagc ccacccgggc acattgccac
     4021 cggacggcag gatgatctgc gatccgatcg actggtagta ctcgaggctc gttcggtgtt
     4081 cgggaatcag ctgaacgtcg cctttgatga gatattcatc cgtattattc ggcagtccga
     4141 tgttccccac aaaccggttg tcgggactcg gacgattggc cgacggcaga ggcacattct
     4201 tcgcaagaac cgccgccgca accgggtccg gcgcgaagtt cggatccaga tccaggcggt
     4261 tgccgggaac aggctggtgc gtaaccggat cgcagacgaa gaacttgccg ccgtaatccg
     4321 tgctggattt atccgcgttc gaaagcttcg tcgcgcatgc tcctaaaccc gccgtcggcg
     4381 ttgtgctcgg cagattctct gagaagttgc cggcccgctg caaagcatcc ggcaccacgg
     4441 tgttaaagtt gtgcgggctg atctgccgca aaccgccata actgccaaag aggaagatct
     4501 tgttcttcca cgccggaccg cccagggtcg ctccaaaacg gttgatgtgc tcgggagtcc
     4561 gggtgtcctg cagatagctg ttcgcgttga agttggtttc acggtgaaat tcgaacactg
     4621 aaccgtgaac ttcgttggtg ccggacttcg tcaggacgga aaccacaccc gcgcccgttc
     4681 gcccatactc ggcgctgaag ttattcgtct ggacattgaa ctggtcaatg gcatccgggt
     4741 tcgggattac attgcccgta tcgcgaacgc cggtcatatt aatgccgccg tcaagatagt
     4801 aggtcacctg gcccaccatg ttgtccgtcg aaccgttgat gatgacatgc tccatcggca
     4861 gcccgatgga attctcgttg ctcacgctct gcacgcccgg cgtgagattc agcaggtcgt
     4921 agacattccg gctcaccaaa ggcaggttct cgatcccgcg attgtcgatc gtggccccca
     4981 gaacggaatc ccctgtattc accagcggca cctcggccgt cacctgaacc actgaggtct
     5041 gaacgccagg atgcagcgtg aaattgatcg ccgcctgttg cgtgccggcc agaacaattc
     5101 cgttctgcac caactgctgg aatccgcctg cattcacttt gacgctgtac ggccccaccg
     5161 gcagaaactc ggcatgatat tgcccctggc cgtcggtcgt tgtcgttcgg gtgaaattcg
     5221 tttgcgtttg cgtgaacaca accgttgcgt tgggaattgc ggccccgctt gaatccatca
     5281 cggtgccgaa ggcggtggcg gtcgataact gcgccgaagc tgcgatcgac atggcacaga
     5341 agactgcgat cagactccac aatcgaatca tcttcatcac gttgaacctc cagaatgttc
     5401 ttgttgctga tttcgttgtt cgtccctgcc tgatctgtca gcgtccgcgt ctccggtcag
     5461 gctcctgttt cttaatcccg aatgcttctg ctccctcacc cggaaggaat ctgcacaaat
     5521 gccggctcca cccccgcgga atgaacgccc acaatcaccg gatccgtatg cgacacacgg
     5581 atctgggcac gcccgttgca catctccacc acacgcgaac ccgtcgtcgt gccaaggttg
     5641 tcctgaagcc gcccgttgcc cgccagcgag aaacacacca cagcccgcga atccaggcag
     5701 cgaacgccgg ccgaatcaac cagagcagca tcaatcgttg tcgcttccga accgcgatcc
     5761 cccagcgaag ctgtaaggcg tgcgggcgaa ccccaggtcc gcgtctcata agtgaacccg
     5821 atgctgtcct caatggaacc gtcatccgtg cgagctaccg cccgcagttc gttctcgcct
     5881 tcacgaaagg caagtgacca tcgcaatccg gcggccggga agttatccgg atccctttgc
     5941 cggacgccga caggcttacc gttgaggtaa agctcgacga cccggcaatt ggagtagacc
     6001 cttacctcgc gttgctgacc cggcttaccc caccgcaccg gccagctatg tccatagata
     6061 tggaccatgg gcttctccgc ccaccacgac tgaaaaaggt agtagctctc cttcggagtt
     6121 agatcgcgcg ctaccaggcc tttttgattc acgcgaggga tcgggttatc gtcccgaagc
     6181 gcggtcgtaa agtccttgaa acaccactgt gctgcgcccg tcatccaggg cagctttgcg
     6241 agtgtcttca gataccagtc gaaaagatcg caggcatacg tctcgctcca gtccccgtcg
     6301 ctggagacgc gcaccggacc cccgctgcgc ttgtaagcaa aaccgacctc cgcagttccc
     6361 ctgcccgtgc gaaccgccgc cagtgcctga tccggatctt cggcatggcg ccccgcatga
     6421 ctatcggcgc cccactcgat gtgcatgaag tgcgggaccg tcccgcgcgc cttctccagc
     6481 gcgctctcat attccgtgta acgaccgctg taccaccccg cccagatcga aggcgaataa
     6541 acat
//
LOCUS       DASZYN010000162         5885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_388738, whole genome shotgun sequence.
ACCESSION   DASZYN010000162 DASZYN010000000
VERSION     DASZYN010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388738"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1309
                     /locus_tag="VHE33_04130"
     CDS             122..1309
                     /locus_tag="VHE33_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVT96669.1"
                     /translation="MRKYRLRFPAVAAALLLLQTLLPGRWLCAQQTTPPGTPQTQPQQ
                     QPADQTAPEAGGPGNDNGPIAIPKKAPTNTPPPPPQPTVKNPPGLGNLSLRVDVPVVN
                     VDVNVMTDKTHQFIPNLKEGNFRVFEDGLPQEIVGFHQVKAPITAVLLVEFASTDWNF
                     IFDMWNSAIAFTEQLRPDDYIAVVTYDMHTTILTDFTQNKMLIQQAVQSLQIPTWQET
                     NLFDAVYETLDRLTRIDGRKYIILVSSGRDTFSKITLDKCLQKIKATPNVTIFSIATG
                     GAGYMGGGGFASGRMNTIDMLQAQNQLQSFARMTGGMYFEPRFTAELPEDFAAINESI
                     RNQYQITYKPSNLKMDGTYRRIKIELVDEEGHPLRMQDQKHKPVKYDIIARDGYKARQ
                     QVE"
     gene            1919..2842
                     /gene="miaA"
                     /locus_tag="VHE33_04135"
     CDS             1919..2842
                     /gene="miaA"
                     /locus_tag="VHE33_04135"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HVT96670.1"
                     /translation="MEPLLVVLLGPTGSGKTALSHTLARRFQGEIVSCDSVAVYRDME
                     IGTAKPSQAERHEIPHHLIDVLPPTHFMTAGDYARLAREALAGIFARRHLPIVTGGTG
                     LYLRALLEGLFAAPQRSEEIRTRLCSSAERYGAGWLHRILRRLDPAAASRIHANDQPK
                     LIRAIEVCLAARLPITQAWQSGRNPLTGFRILRIGLDPPRPQLYLRLNTRAEVMFSQG
                     LVQETRNLIAKYGDSPRAFDSLGYRQARSVLRGETTEPIAIAATAQGHRNYAKRQLTW
                     FRREPDVRWLPAFGDDPAIASQVIGLIEKNL"
     gene            2922..4403
                     /locus_tag="VHE33_04140"
     CDS             2922..4403
                     /locus_tag="VHE33_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96671.1"
                     /translation="MQNRLLKIPSAFAPLLLTPLVLLVHGYHPFAGDAGIYVAGVRHI
                     LDPSLYPVNAAFPTAFTRMSIFPWILAALVRLTHLSLPWMLLAAHLASIGLFLFACRQ
                     LASRLFPGESARWCAVLFAAACFTLPVAGTALFIMDPYGTARSFSTPLGLLALTAILD
                     RARFRAAVLLLFAALIHPLMGAYASIFVLLYALIAAKRARLAVLACCTLFAAAAAVFL
                     LTHPSANSQSYLEALDLPQHSFLFLSRWHWYEVLGLLLPLLLYGHGLRKLRPAGPKHA
                     LCLACLCCGTTSLLVAAFFVSPAGPWTLVPIQVLRSFHLIYAAGIVLCGGILAKLLSR
                     SRLAGVSLIALLFAGVFAAEPFAWPACNRIEWPASTPANPWQKAFLWIRFNTPRNAVF
                     AYSPQLVYLPEEDEQGFRAIAERDHLGDDKDAGIVAVIPQLADRWALQRNAQLDANSM
                     TDSQRIAALKPLGATWMLLPPQTGTDLPCPYRNSVVAVCRMDE"
     gene            complement(4493..5455)
                     /locus_tag="VHE33_04145"
     CDS             complement(4493..5455)
                     /locus_tag="VHE33_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96672.1"
                     /translation="MSLQFRKNIALQWTANLGLCIGLLAGAGLAQAQVATRTQVSTQK
                     NTGTLTFTAKVADVQGTPVSEGSVSFETVKGSLGSVFVKDGTATLNLTHPPQWATTVT
                     AVYHGNTVFANSAASTGVVPAATGSVPGFTVTANPSSLSLTPGQYGTVNLAVTSQNGF
                     SETVNLSCSGLPGGTDCIFNPVVATPPANGSVNSALQITTQAASGVLAKNEHPGRAGT
                     LYAVLIPGVLALGGFGALRRKNHRALRVLGVMMLLATGTLGLTACNVRYRYLHYQPSP
                     NPGTAAGSYTIVVAAYSTNGTAITNATSSDPGCSGAVCVALTVQ"
     gene            5668..>5885
                     /locus_tag="VHE33_04150"
     CDS             5668..>5885
                     /locus_tag="VHE33_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96673.1"
                     /translation="MATSLSARPQLQFLSAQIEQLKQKGTHFRLRVLDDAQAPVCTYD
                     GKRVINLASNNYLGLCNHPKLREAAIQAT"
BASE COUNT         1097 a         2089 c         1485 g         1214 t
ORIGIN      
        1 atcagcgctg ccacgccccg gcggatatct ttcggttgct gatacacgtc catagactat
       61 gctgctccca cgcttgccgc aaacctgtca gcggcacgaa gatggttcca gaggttgttc
      121 tatgaggaaa taccgcctgc gtttcccggc cgttgctgcc gctctgctgc tcttacagac
      181 gcttttgccc ggtcgctggt tatgcgctca gcaaactacg cctcccggca cgccgcaaac
      241 ccagcctcag cagcagcccg ccgaccagac ggcgcccgaa gccggtggcc ctggcaacga
      301 caacggcccc atcgcgatcc cgaagaaggc tcccacgaac acgccgcccc cgccccccca
      361 gcccaccgtc aaaaacccgc ccgggctcgg caatctgtct ctccgcgtcg atgtcccagt
      421 ggtcaacgtc gacgtcaatg tcatgactga caagactcac caattcatcc ccaatctcaa
      481 agagggcaac ttccgcgtct ttgaagatgg tttgccccag gagatcgtcg gttttcatca
      541 ggtgaaagct ccgatcaccg cggttctgct ggtcgagttc gcctccaccg actggaactt
      601 catctttgat atgtggaatt cggccattgc cttcactgag cagttgcgcc cggacgacta
      661 cattgcggtc gtgacgtacg acatgcacac cactatcctg actgacttca cccagaacaa
      721 gatgctgatc cagcaggccg tccagtctct ccagattccc acgtggcagg aaacgaacct
      781 cttcgatgcc gtctacgaaa ctctcgaccg cctcacccgg atcgacggcc gcaagtacat
      841 catccttgtc tcctcggggc gcgacacctt ttccaaaatc acgctggata aatgtttgca
      901 gaagatcaaa gcaacgccca acgtgaccat cttttccatc gccaccggcg gcgcgggcta
      961 catgggcggc ggcggatttg ccagcggacg catgaacacc atcgacatgc tgcaggctca
     1021 gaaccaattg cagagctttg cccgcatgac cggcggcatg tatttcgagc ctcggttcac
     1081 cgcagaactc cccgaggatt tcgccgcgat caacgagtcc attcgtaacc agtatcagat
     1141 tacttataag ccttcgaacc tgaagatgga cggcacctac cgtcggatca agattgagct
     1201 ggtcgacgag gaaggccacc ccctgcgcat gcaggaccag aagcataagc cggttaagta
     1261 cgacattatc gctcgcgacg gctacaaagc tcgacagcag gtcgaataga ccgccatcaa
     1321 agccgattct tcctgattga tttcttctgg acccgaaaag ccacttatta ccaaaacggt
     1381 acagaagttt cggtacctcc gtcacgagta acccgctgag agggtacttt ctcgttggct
     1441 ctgctgcctt gaagtgctat agatggacca ttgcctcggg actgatactg tgggtgccga
     1501 ggcgaatggc tctcttttcc gaggaagttt gggcaatgca tgacctgatt tttgctgtcg
     1561 ctttcgttgc aatggtcgca actcccgcca tggtcgccgc catcggtggc cgcaaagaat
     1621 tcgatccagg cccggttgct cccccaatcc gcagccggac cacggtccca cccattcgtg
     1681 tccagccctt gaccccgacg gttcatccgc gcaccccccg gagactcgtc accgaacgcc
     1741 gaccctgcgt gattacagac ggtcccacgc tacccatgcg caacgcgcgg ggcatgtcga
     1801 accgctaaat cagaagcccc tgaagctcgg tttcggggat agggggcgca ctctggcgta
     1861 acgcccccgg gaacgcacgc accaccgcat cttccactgc taacctgact ccgtctgtat
     1921 ggaacccctt cttgtggtgc tgctgggacc caccggcagc ggcaaaaccg cgctctccca
     1981 caccctcgcc cgccgtttcc agggagagat cgttagttgc gactcggttg ccgtctaccg
     2041 cgacatggaa atcggcaccg ccaaaccatc ccaggccgaa cgtcacgaga ttccgcacca
     2101 tctgatcgat gttctgcccc caacccattt catgacggcg ggcgactacg cccgtctcgc
     2161 ccgcgaagcc ctcgccggca tcttcgcccg ccgtcacctc cccatcgtca ccggtggcac
     2221 cggcctctat ttgcgcgccc ttctggaggg cctcttcgcc gctccccagc gctcggaaga
     2281 aatccgcacc cggctttgca gcagcgccga acgctacggg gcaggatggc tccaccgtat
     2341 cctccgccgc ctcgacccgg ccgccgccag ccgcattcat gccaacgatc agcccaaact
     2401 gatccgcgcc atcgaagtct gtcttgccgc gcgcctgcca atcacccagg cttggcaatc
     2461 cggccgcaat ccgctcaccg gcttccgcat cctccgcatt ggtctcgacc cacctcgccc
     2521 gcagctctac ctccgtctca acacccgcgc cgaggtaatg ttttcccagg gtctcgtcca
     2581 agagactcgc aatttgatcg caaaatacgg cgattcgcct cgcgccttcg attcgctggg
     2641 ctatcgccag gcgcgctcgg ttttgcgtgg ggaaaccacg gaacccatcg caatcgctgc
     2701 cactgcccag gggcatcgca actacgccaa acggcagctc acctggtttc gacgcgagcc
     2761 tgacgttcgc tggctgcccg ctttcggcga cgatcctgcc atcgcatccc aggtcatagg
     2821 actcatagaa aagaatctct aatcgtatgc ccgccgcgcc tgcaccgcga acatcgcttc
     2881 accaaccagg ccgcttcctg aggtctacgg gaacgccgcg attgcaaaat cgcctgttaa
     2941 aaatcccgtc ggctttcgcg cctctgctcc tcacgccgct cgttctcctg gttcacggct
     3001 accatccctt tgctggcgac gcgggcatct acgtcgcggg cgtccgtcat atcctcgacc
     3061 cttctctgta tcccgtgaat gcagcctttc ccaccgcatt cacgcgtatg tccatcttcc
     3121 cgtggattct cgccgcgctg gtccgcctca cacacctctc actcccgtgg atgcttctgg
     3181 ccgcacatct cgcttcgatt ggcctgttcc tcttcgcctg ccgccaactc gcatctcgcc
     3241 tctttcccgg cgaaagcgcg cgatggtgcg cggtcctctt cgctgccgct tgtttcacgc
     3301 ttcccgtcgc cggcaccgcg ctctttatca tggatcccta tggcaccgcg cgatccttct
     3361 ccacccccct gggccttctt gccctcacgg cgatccttga ccgtgccagg ttccgcgctg
     3421 ccgtgctgct cctgttcgcc gccctaatcc accctcttat gggcgcatat gcctcaatct
     3481 tcgttcttct ctatgctttg atcgccgcaa agcgcgcgcg gcttgcggtt ctcgcctgct
     3541 gcactctatt cgcagcggct gccgccgttt ttctccttac ccatccatct gcaaactccc
     3601 agtcatacct cgaagccctc gatctcccgc aacacagttt cctattcctg tcgcgatggc
     3661 actggtatga agtgctggga cttctcctgc cgctgctcct ctatggccac ggtctgcgca
     3721 aactccgccc ggccggccca aagcacgccc tctgcctcgc ctgtctctgt tgcgggacca
     3781 ccagcctgct cgtcgctgcg tttttcgttt cgcctgccgg accctggacc ctggttccca
     3841 ttcaggttct ccgcagcttc cacctcatct acgctgccgg aatcgtcctg tgcggcggca
     3901 tcctcgcgaa gctgctgtct cgctcgcgcc tcgccggagt ctccctcatt gccctgcttt
     3961 ttgctggagt gtttgcagcc gagcccttcg cctggccggc ttgcaaccgc atcgaatggc
     4021 ccgcctcaac cccagccaac ccatggcaaa aagcctttct ctggatccgc ttcaacaccc
     4081 cccgcaacgc tgtcttcgcc tatagcccac aattggtcta cctgcccgaa gaagatgagc
     4141 agggctttcg cgcgattgcc gaacgcgacc atctcggcga cgacaaggac gccggcatcg
     4201 tcgccgtcat cccgcagctc gcggaccgct gggctctcca gcgcaacgct caacttgatg
     4261 cgaattcgat gaccgactcg cagcggatcg ccgcactcaa gcccttaggc gcgacgtgga
     4321 tgctcctgcc gccacaaaca ggaaccgatt taccctgtcc ctaccgtaat tcggtggttg
     4381 cagtctgccg gatggacgaa tgacattatc gaccgggaca ttcgcgctca ggtatgaagg
     4441 cgcatctgcc gggtagcagg gctgccataa accttattcc cgattccctg ttttactgca
     4501 ccgtcagcgc aacgcacacc gcgcccgaac agcccggatc cgacgaggtc gcgttggtga
     4561 tcgcggtacc attcgtcgaa taggccgcaa ccacaatggt gtagctcccg gcggccgtcc
     4621 ccgggttcgg cgaaggctga tagtgcaggt aacggtagcg cacattgcac gccgtcaatc
     4681 ccagcgtgcc cgttgccagc agcatcatca ctccaagaac acgcagcgcg cggtgattct
     4741 tcctccgcag cgctccaaag ccgcccagcg ccagcactcc ggggatcaga acagcgtaaa
     4801 gtgtgcccgc acgcccggga tgttcattct tcgcaagaac gccggacgct gcctgcgtcg
     4861 tgatctgcag cgcgctgttc actgagccat tcgctggcgg cgtggcaaca acggggttga
     4921 atatgcaatc ggtaccgccc ggcagtcccg agcacgacag attgaccgtc tcagaaaaac
     4981 cgttctgcga ggtcacagcg agattgaccg taccgtactg gcctggcgtc agcgataacg
     5041 aggacggatt cgctgtcact gtgaaacccg gtacgctgcc cgtcgccgcc ggcacaacgc
     5101 cagtgctcgc tgccgaattc gcaaacacgg tattcccgtg ataaaccgcg gtaaccgtcg
     5161 tcgcccactg cggcggatgc gtcaagttca gcgtcgccgt accgtctttc acaaagaccg
     5221 atcccagcga tcccttgacg gtctcaaagc tcaccgaccc ctcggagacc ggcgtcccct
     5281 ggacgtccgc cactttggcg gtgaatgtca gggttccggt gttcttctgc gtcgacacct
     5341 gcgtgcgggt agccacctgg gcctgcgcca gtcccgcgcc agccaaaagg ccgatgcaaa
     5401 gtccgaggtt ggcggtccat tgaagggcga tgtttttccg gaactgcaaa ctcattcgtg
     5461 aagaactctc tccagtcagg cgtccgcttc aaagcatcgc tgaacggccg ctcaatcctg
     5521 cctggccgaa tccgtcgagt gctcctgcgg tggaaaactc attgtatctg accgtatgca
     5581 cctgcaacag gtgagcacat ccgccgcccg cttctgtctg gagttaggat cgaaggttgc
     5641 ccatttttgc agttcccgag gtccttcatg gccacgtcgc tctccgcgcg ccctcagttg
     5701 cagttccttt ctgcccagat cgaacagctc aaacagaagg gcacccattt ccgcctccgc
     5761 gtgctcgacg acgcgcaagc gcccgtctgc acgtacgacg gaaaacgcgt catcaatctc
     5821 gcctccaaca actacctcgg cctctgcaac caccccaaac tccgcgaagc cgccatccag
     5881 gccac
//
LOCUS       DASZYN010000163         5119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_70908, whole genome shotgun sequence.
ACCESSION   DASZYN010000163 DASZYN010000000
VERSION     DASZYN010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70908"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VHE33_04155"
     CDS             <1..303
                     /locus_tag="VHE33_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HVT96674.1"
                     /translation="YYVEVRDGPLVCRHRPSVDVLFRSAARYAGPNAVGVILTGMGDD
                     GARGMQEMHQAGATTIAQDEATCVVFGMPKEAIKLNGVDKVMPLESIAGGILHAAR"
     gene            840..1937
                     /locus_tag="VHE33_04160"
     CDS             840..1937
                     /locus_tag="VHE33_04160"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HVT96675.1"
                     /translation="MKELLWGSRPPDMEGAEAPPPPFALGTYDPFAAPEPSEALDLRG
                     FIHILSKARYLTHVRERVDWKTGIARWTRSRQKPLLFENVKEYPEQRVFTNGLAHPSC
                     IALALGFGSGLPMSSVLIRARKRLREPMAPKMVPTGPVIENVVPSSMIDFFQFPVPQW
                     HEREGSRYLGTWHLNVSRDPDTGHRSVGMYRMKALGPKQASINAPRRSDLARHIARAE
                     SRKKELPMAVAIGAPEATVIAARTACPHGMDAFDLAGALQQKPVELIQCGHSEAPAYS
                     EIVIEGFVHPEARVDDGPVFDYEGKPHAHSKAYVFEATRLMHRDEPIFRASALGKPGS
                     EDYQVQAFLDELKLRNANGSRLWNFVQSLFS"
     gene            complement(2008..3567)
                     /locus_tag="VHE33_04165"
     CDS             complement(2008..3567)
                     /locus_tag="VHE33_04165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HVT96676.1"
                     /translation="MSPKAIPTAFFLLAGIAVMPAARAQSNNYTQTNLASNVPGVALT
                     MDRDLLHPWGVATSANQPFRIALNGKGQFRSYDAAGNQRDPRAAIDVPPGGIVPPNPT
                     GVAANTTGLFVPSTSLSTPFLFATRQGTISTEYADDRGDIKTTTILVIDHSSLGAEYT
                     GLAVLAPDCCAPYLAVADFHRGFIETFTSFFAPLGIAGAFIDPNLPAGYAPWNLQVVG
                     DKLFVAYALQDAAQHDPVAGDGNGIVDIYNLDGSFVRRFVSNGPLNVPSGIVQASANF
                     GAFSNAILIGNAGDGVINAFDPASGDLLGKLKDGNTNVITNAHLHGMTFGDGTVGDAN
                     TLYLTSALPSGADGLFAAISVNTSGTGPNFNLNSDRQSAIVSPGQSATFSLTATPVAN
                     FRGAFVFTCNVPPAVTCTIGPPTVDVATGAATVTVTASAAATPSANTMAAFALPGVLL
                     AGLGLRKRRSAWRFVMLAIGMLALAAAGATGCGGSGGVHMTPQPVPHSFSVAASAASV
                     SRVTTLTLNVQ"
     gene            complement(3778..>5119)
                     /locus_tag="VHE33_04170"
     CDS             complement(3778..>5119)
                     /locus_tag="VHE33_04170"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVT96677.1"
                     /translation="SVSVLLLIPASCSGHRKSSHGLFVDPGIAKTIDSIQAIDNHAHP
                     VLAPPLDATEKEFDALPVSNLEPQSDPPALRPDFPLLRQAWNALYGIELQPPLDAAGA
                     KRLGEARARAKQAHGEQYPAWVLDQAKIGTMLANRVAMGRGVEPPRFQWVPYVDALLF
                     PLDNSGMAAASPDRRAFFALEDALRHRYLQALGLKSPPPTLDGYLKKVVTPTLERQKS
                     QGAIAEKFEVAYLRSFEFTNPPRSQVSRIYARWVREGSSRAPDLSDYKLLQDYLFRYI
                     ARECGRLGLVVHLHAMAGGGSYFSVAGVNLLLLEPILNDPHLRKTKFVLLHGGWPYVR
                     EIGSLLQKPNVYLDISGQDLLMTPRTESSWLREWLEFEPEKVLFGSDGYPYSSELGWA
                     ESTWIASHNARLALGVALTRMLRDGDISRDRANEIARLVLRGNAEHLYFPSASH"
BASE COUNT         1000 a         1377 c         1802 g          940 t
ORIGIN      
        1 tactacgtgg aggtacgaga cgggccgctg gtgtgccggc atcggccgtc ggtggatgtt
       61 ctgttccgct cggcggcacg gtatgcgggg ccgaacgcgg tgggcgtgat cctgaccggg
      121 atgggagacg atggcgctcg cgggatgcag gagatgcatc aggccggggc gaccaccatt
      181 gctcaggatg aggcgacgtg cgtcgtgttt ggaatgccga aggaggcgat caaactgaac
      241 ggcgtggata aggtgatgcc gctggagagc atcgcgggag gaatcctgca cgcggcgcgc
      301 tgacgcgtcc gctccggcgt gcgacgaaat ttgacgtgcg gagcggtgag aagcaggtcc
      361 ctccgcttcc ccttcgttat gctcagggtt cggccgggat gacaaggtga aggaaacaga
      421 aacggcacac gagcctgggg tcggtggcgt tccgcgttca tagcaagaag ttattcaccg
      481 taacacccta acgggggagt gccgtgtgtt gtccgattgc ggtacattct ccccggaaag
      541 ttgtagattg ccgcactggt ttggtgacgg tatccaccga agcgttcgct ataacacaag
      601 agaatgcgtg gtccgaagtt cgctcgagcg acctccgagc cacggctcct ccccttttac
      661 gttcatctgc cgtcccggtt cccgtcattt caagatcgcc ggtgcggttt cgctggcctt
      721 ttgaaggagc taccgggttt gcgtctcatc tcccacccca tcgttgcgtc gcaagccggg
      781 tctcgaacat cgacgttgct gtcttccgta cacggacaca aatcgcgagc tgcaaggcca
      841 tgaaggaact tctgtggggc tcgagaccgc cggatatgga aggcgcggaa gcgccgccac
      901 cgccgtttgc gttgggcact tacgacccct ttgcagcgcc ggagccctcc gaggcgctcg
      961 atctgcgggg cttcattcac attctcagca aggcgcgcta tctgacgcat gtccgggagc
     1021 gggtggactg gaaaaccggc atcgcgcgct ggacgcgatc gcgacaaaag cctttgctct
     1081 ttgaaaatgt gaaggagtat ccggagcagc gggtgttcac gaacgggctg gcgcatccat
     1141 cgtgtattgc gctggcgctg ggatttggtt cggggctgcc gatgtcgagc gttctgatcc
     1201 gggcgcgcaa acggctgcgg gaaccgatgg ctccgaagat ggtgccaacc gggccggtga
     1261 tcgagaacgt ggttccgtcg agcatgatcg acttcttcca gtttccggtt ccgcaatggc
     1321 acgagcgcga gggcagccgc tatctgggca cgtggcacct gaacgtgagc cgcgatccgg
     1381 atacggggca tcgcagcgtg gggatgtatc gcatgaaggc gctcggaccg aaacaggcga
     1441 gcatcaatgc cccgcggcgg agcgatctgg cgcgacacat tgccagggcc gagagcagga
     1501 aaaaggaact gccgatggcc gtggcgattg gcgctcctga agcgacggtg attgcggccc
     1561 gcactgcatg cccgcatggg atggatgcct tcgatctggc gggagctttg cagcagaagc
     1621 cggtggagct gatccagtgc ggacactccg aggcgcctgc gtactctgaa attgtgatcg
     1681 agggtttcgt gcaccccgag gcgcgggtcg acgatgggcc ggtgttcgac tacgagggga
     1741 agccgcatgc gcattcgaag gcgtatgtgt tcgaggcaac gcggctgatg catcgcgacg
     1801 aaccgatctt tcgcgccagc gctttaggga agccgggctc ggaggattat caggtgcagg
     1861 cttttctgga tgaactgaag ctgcgcaatg caaacggttc gcgtctttgg aatttcgtac
     1921 agagtctgtt cagttagcgg attggatcga agcaaaaaag atgcggcccg tcatttcgac
     1981 gggccgcaac attttgatcg tccgcactca ctgcacgttg agggtcagcg tggtgacgcg
     2041 agagaccgaa gcggcgctgg ccgcgacact gaaggagtgc ggcacgggct gcggggtcat
     2101 gtgaacccct cctgagccgc cgcagccggt ggctcctgcc gctgcgagcg cgagcatgcc
     2161 gatcgccagc atcacaaaac gccaggcgga acggcgtttg cggaggccga gacccgccag
     2221 cagaacgccc ggcagagcga acgccgccat ggtattcgcc gaaggtgtcg ctgccgccga
     2281 ggcggtaacg gtgacggtgg ctgctcccgt ggcgacatcg actgtgggcg ggccgatcgt
     2341 gcacgtgacc gctggcggaa cattgcaggt gaagacaaag gctccgcgga agttcgcaac
     2401 gggtgtcgcc gtgagggaga aggtcgcgga ctggcccgga ctgacgattg cgctctgccg
     2461 atcggagttg aggttgaagt tgggaccggt accggaagtg ttgacggaga tggcggcgaa
     2521 caatccgtcc gcaccgctgg gcagagccga ggtgagatag agcgtattgg cgtccccgac
     2581 ggtgccgtcg ccaaaggtca tgccgtgcaa atgggcattg gtgatgacgt tggtattgcc
     2641 gtctttgagc ttgcccagaa gatcgccgct cgcgggatcg aaagcgttga tgacgccgtc
     2701 gcccgcgttg ccaatcagga tggcgttact gaaggcgccg aagttggcgc tggcctgaac
     2761 gattcccgac ggcacgttca ggggcccgtt ggaaacaaac ctgcgaacga agcttccatc
     2821 gaggttgtaa atgtcgacga tgccgttgcc gtctccggcg accgggtcat gttgtgccgc
     2881 atcctggagc gcataggcga cgaacagctt gtctccgact acctggagat tccacggagc
     2941 gtagccggcg ggcagattgg gatcaatgaa ggcaccggcg atgccgagcg gagcgaagaa
     3001 tgaggtgaag gtctcgatga aaccgcgatg gaaatcggcg acggcgagat agggcgcaca
     3061 gcagtccggt gcgaggacgg cgaggccggt gtattcagca cccagcgacg agtgatcaat
     3121 cacgagaatg gtggtggttt tgatgtcgcc gcgatcgtcg gcgtactcgg tcgagatggt
     3181 tccctggcgg gtggcgaaca gaaagggagt ggagaggctg gtggagggaa caaagaggcc
     3241 ggtggtgttc gcggcgactc ccgtgggatt gggggggaca atgccgccgg ggggcacgtc
     3301 gatcgcggct ctgggatcgc gctggttgcc ggctgcgtca tagcttctga actgaccctt
     3361 tccattgagg gcaatgcgaa agggctgatt ggccgaggtc gcgacacccc acggatgaag
     3421 aaggtcacga tccatggtga gagccacgcc ggggacgttg gaggcgagat tggtctgcgt
     3481 gtagttgttg ctctgcgcgc gggccgcagg catgacggcg atgccggcga gaagaaaaaa
     3541 ggctgtgggg atcgctttcg gactcacggt cgtttcctcg ttctgaaaaa tggaagcgcc
     3601 tggagcgagc ggggcccggg caatgaccat cgtccttgca tcactgccgc tgagagaagg
     3661 accacagggg cttacagagc ggattgtagg gattggattc agaaccgatg tcagggattc
     3721 gtcaacgggg cgtcaaactc tgggaattgc gttgttggct gtgacgaatg gccactttca
     3781 atggctggcc gacgggaaat agagatgttc ggcattgccg cgcagaacca ggcgagcgat
     3841 ttcgttggcc cgatcgcggc tgatatcgcc gtcgcgcaac atacgggtga gggcgacacc
     3901 gagcgccagg cgcgcattgt gactggcgat ccaggtggac tcggcccagc ccagctccga
     3961 tgaatacgga tagccatcgc tgccgaagag caccttctcc ggctcgaact ccagccactc
     4021 gcgcagccag ctcgactcgg tgcgcggcgt catgagcagg tcctggccgg agatgtcgag
     4081 atagacattg ggcttttgca ggagcgatcc aatttcgcgc acatagggcc agccgccgtg
     4141 caggagaacg aatttggttt tgcgcaggtg gggatcgttg aggatcggct ccagcaggag
     4201 cagattcacg ccggccacag aaaagtagct gccgccgccg gccatcgcat ggagatgaac
     4261 caccagtccg agacggccgc actcgcgtgc gatgtagcga aacaaataat cctggaggag
     4321 cttgtagtcg ctgaggtcgg gtgcgcggga cgagccttcg cgaacccagc gagcgtagat
     4381 ccgggagacc tgggagcgcg gagggttggt gaactcgaac gaacgcagat aagcgacttc
     4441 gaatttctcg gcgatcgcgc cctgggattt ctggcgctcg agggtgggcg tgacaacttt
     4501 tttgaggtat ccgtcgagcg tcggtggcgg cgacttgaga ccgagagcct ggagataacg
     4561 atgacgcagc gcatcttcga gagcaaagaa ggcgcggcgg tcgggtgagg ccgcggccat
     4621 tccgctgttg tcgagcggga acagcagggc gtcgacgtaa ggtacccact ggaaacgggg
     4681 cggctccacg ccgcggccca tggcgacgcg gttcgccagc atggtgccga tcttcgcctg
     4741 atcgagcacc cacgccggat attgttcgcc atgggcctgc ttcgcgcggg cccgcgcctc
     4801 gccgaggcgt tttgccccgg cagcgtcgag cggaggctgc agctcaattc catagagtgc
     4861 gttccaggcc tggcggagaa gcgggaaatc agggcgcagg gcaggcggat cggattgcgg
     4921 ctcgaggttc gacaccggca gcgcgtcgaa ttctttttct gttgcatcga gcgggggtgc
     4981 gagcaccgga tgggcatggt tgtcgatggc ctggatcgag tcgatggtct tcgcgatgcc
     5041 gggatcgaca aagaggccgt gactggattt gcgatgtccg gaacaggacg ccggaatgag
     5101 aagcaggacc gaaacagaa
//
LOCUS       DASZYN010000164         2825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_70932, whole genome shotgun sequence.
ACCESSION   DASZYN010000164 DASZYN010000000
VERSION     DASZYN010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70932"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VHE33_04175"
     CDS             <1..585
                     /locus_tag="VHE33_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96678.1"
                     /translation="TTTQVTYRNGHEVAATTEANERELERIGLTTKGEFGPILVIVVS
                     DALHNKLYWDHWEMGGAGRIAVLRYTVPQEGSHYSVSYPSESGRQLISPAYHGVLAFD
                     PETGTVFRLTVVADTQPPYQHIQVGIVVEYGSVLLGDKTYICPVHAVALSRQPTEGTN
                     VTSPPLRTCLNDVKFTGYHLFHADARIVSSKDEP"
     gene            717..2516
                     /locus_tag="VHE33_04180"
     CDS             717..2516
                     /locus_tag="VHE33_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVT96679.1"
                     /translation="MIRSRRFFACCALLAVVSFLTGAAQTQSSSAQSPPTQPAASPAT
                     FHTGTNLVLVDVVVRDKGKPVTGLAAADFRVLEDNQSQAITTFEEHKATDAVKLSEPP
                     KLPPHVYADFPRYRTASAANILLLDALNTPMQDQKSVRQKMIRYLGSIPPGTQIAVFT
                     LASRLRMVSGFTTDIGTIERALSQEHTPVEKSVLVHPEDNKIENDIAEAMRGAATDGQ
                     AADLARAIEDFNDDREAFQVQQQAGITIAALTQLGRYLSTIPGRKNLIWFSRAFPLRF
                     MVDNLRQDQDYGKPMQVMEALLARARVAVYPVDAGGVMNLGDTNIANVTAPTDLGGTR
                     DLPVAVAEGVHDDISVPQRWGNEQAAMKDLATATGGEAFVNTNDVGRVVEKAIVEGSN
                     YYTLGYTPRDVREDGAWHTIRVQMPEGKYDLAYRRGYYATVANQPGQAAGPNPMTAAM
                     EPGVPPLSQVIFEARVLPAGDPDLRGLQPTPGAAGRPPSPLKTPVTRYFIDYSIDLHE
                     LELKDLPDGRHQAELEITQALYDATGKRLNATDAGLEVTMTPEQMTRDLKSGVHVRQE
                     IDVPADNVSLHLGVRDAASGRIGTIQVPVGSAR"
     gene            complement(2571..>2825)
                     /locus_tag="VHE33_04185"
     CDS             complement(2571..>2825)
                     /locus_tag="VHE33_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96680.1"
                     /translation="RNAALHAVALATGLRFHFERTESSPLPSLGDVTFYINTGSRVSC
                     AVVPESRLRAGADPLAGLSGAMQRIVTEYRLNLGRSGNKA"
BASE COUNT          582 a          803 c          928 g          512 t
ORIGIN      
        1 accacaactc aggtgaccta tcgcaatgga cacgaggtcg ctgcaacgac agaagccaac
       61 gagcgtgagc tcgagcgtat tgggttgacg accaaaggag agttcggccc catcctggtg
      121 attgtggtca gcgacgcgct ccacaacaag ctctactggg atcactggga gatggggggc
      181 gcgggccgca tcgcggtgct gcgctatacc gttccccagg agggttctca ctacagtgtt
      241 tcctacccct cagaatccgg caggcagctc atctccccgg cttatcacgg ggttctcgcc
      301 ttcgacccgg agaccggcac agttttcagg ctaaccgttg tggccgatac gcagcctccc
      361 tatcagcaca tccaggttgg catcgtggtc gagtatggtt cggttctgct tggcgacaag
      421 acctacatct gtcccgtgca cgctgttgcc ctctcccgcc agccgaccga gggaacgaat
      481 gtgacctcgc ctccgctgcg cacttgtctc aacgatgtga aattcaccgg ctatcacctg
      541 tttcacgccg acgcccgcat cgtttcttcc aaagatgagc cttgattccg gcgcgcagag
      601 tagcgagagc tgccgttttg ttagcatggt cggacgccgc tgagaccatg cgagatttgc
      661 tgagcaacgg gtcttctggc gacggctata cgtccgtggc ggcaagggaa aggccgatga
      721 tccgctcccg aaggttcttc gcgtgctgtg cacttctggc ggtggtctcg ttcctgactg
      781 gcgcagcgca gacgcagtcg tcgtccgcgc aaagcccacc aacccaaccg gccgcgtcgc
      841 ctgcgacgtt ccataccggg accaatctgg tgctggtgga cgtggttgta cgcgacaaag
      901 gaaagcccgt cacgggtctg gcggcggccg acttccgggt cctcgaagac aatcaatccc
      961 aggccatcac cacgtttgag gagcacaagg cgacggatgc ggtgaagctg agtgaaccgc
     1021 cgaagctacc gccgcatgtg tacgccgatt tcccgcgtta ccggactgcg agcgccgcga
     1081 acatcctgct gctggatgcg ctgaacacgc cgatgcagga tcagaagtct gtgcggcaga
     1141 aaatgatccg gtatctcgga agcattccac cggggacgca gattgcggtc ttcacgctgg
     1201 cgtcgcggct gcgcatggtg tcgggattca cgacagacat cggcacgatc gagcgggcgc
     1261 tgagccagga acatacgccg gtggagaagt cggtcctcgt ccatccggag gacaacaaga
     1321 tagaaaatga catcgccgaa gcaatgcggg gcgcggcaac ggatgggcag gccgccgatc
     1381 tcgcacgggc cattgaggat ttcaacgatg accgggaggc gtttcaggta cagcagcagg
     1441 ccgggatcac catcgcggct ctgacccagc tggggcgcta tttgagcacg attccgggta
     1501 gaaagaatct gatctggttt tcaagagctt ttccgctccg tttcatggtc gacaatctgc
     1561 gccaggatca ggactacggc aaacccatgc aggtgatgga ggccttgctg gcgcgcgcga
     1621 gggtggcggt gtatccggtg gatgccggag gagtgatgaa tctcggagac accaacattg
     1681 ccaatgtgac tgcaccgacg gatctgggcg ggacgagaga tttgccggtt gcggtggcag
     1741 agggggtgca tgacgacatc agtgttccgc aacggtgggg aaacgaacag gccgcaatga
     1801 aagatctggc cacggcgacc ggcggcgagg cgttcgtcaa tacaaatgat gtagggcgcg
     1861 tggtggagaa ggccatcgtc gaagggtcga actattacac gctgggctat accccccgag
     1921 atgttcgcga ggacggagcc tggcatacga tccgggtgca gatgcccgaa gggaagtatg
     1981 accttgccta tcggcgcggc tattacgcga cggttgcgaa tcaaccagga caggcggcgg
     2041 gaccaaaccc gatgacggca gcgatggagc cgggtgttcc gcctttgtcc caggtgatct
     2101 tcgaagcgcg ggtgttgccg gcgggcgatc cggatctgcg cgggttgcag ccgacgccag
     2161 gcgcagcggg aaggccgccg tcgccgctca agacgccggt gacgcgatat ttcatcgact
     2221 actcgatcga tctgcatgag ctggagctga aagacctgcc ggatggccgg caccaggccg
     2281 agctcgagat tacccaggcg ctctatgatg cgactggaaa acgtttgaac gccaccgatg
     2341 cagggctcga agtgacgatg acgccggaac agatgacgcg cgatttgaag agcggagtgc
     2401 acgttcggca ggagatcgac gtaccagcgg acaatgtatc gctgcacctg ggcgtgcgcg
     2461 atgctgccag cggcaggatt ggaacgatcc aggttccggt cggctctgcc cggtagctga
     2521 acgcgccgcg aagtgcgggt gcaagccgat aagcgggggc gaatgaagta tcaggctttg
     2581 ttgcctgaac gtccgaggtt aaggcggtac tcggtgacaa tgcgttgcat cgcgccgctg
     2641 agcccggcaa ggggatcggc gccggcgcgg agcctcgatt caggcaccac ggcgcaactg
     2701 acgcgagagc cggtattgat atagaaagtg acgtctccga gagacggcag cgggctcgat
     2761 tcggttctct cgaagtgaaa gcgcagcccg gtagccagcg cgacggcgtg aagcgcagca
     2821 ttgcg
//
LOCUS       DASZYN010000165         5411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_456849, whole genome shotgun sequence.
ACCESSION   DASZYN010000165 DASZYN010000000
VERSION     DASZYN010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456849"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VHE33_04190"
     CDS             complement(<1..989)
                     /locus_tag="VHE33_04190"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HVT96681.1"
                     /translation="MNSDLHLWLIPLVPFAGFLVNGLLGRKLPKALVTTIALIASLVP
                     AIQVAGIVTKFSSLTLPHIEQLGTWIVSGSFHAGFAFQLDQLTLVMLCVVTGVGFLIH
                     VYSVGYMAEEEGYWRFFAYMNLFLFFMLTLVLAENFLLMFVGWEGVGLASYLLIGFYF
                     TRDSAANAGKKAFIVNRIGDFGFLIAMFLLIAHFGSLSFSDVFSQIGAHPEWQSGFLT
                     AIALFLVLGATGKSAQIPLYVWLPDAMEGPTPVSALIHAATMVTAGVYMVARTHVIFD
                     RSPFALAVVATIGVATAFFAATIGILQTDIKRVLAYSTISQLGYMFLGCGVAA"
     gene            complement(986..1306)
                     /gene="nuoK"
                     /locus_tag="VHE33_04195"
     CDS             complement(986..1306)
                     /gene="nuoK"
                     /locus_tag="VHE33_04195"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004321.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HVT96682.1"
                     /translation="MVPIAYYLVLSAILFSIGVAAFLIKRNIITVFMSIELMLNAVNL
                     AFVAFSRQWSQTTGHLQVNGQIFVFFVMVVAAAEAAVGLAIIIAVFRARNTLNVDQID
                     LMKL"
     gene            complement(1310..1804)
                     /locus_tag="VHE33_04200"
     CDS             complement(1310..1804)
                     /locus_tag="VHE33_04200"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HVT96683.1"
                     /translation="MHLALFIFFAVLCVAGALNLLLQRHPINSALSLIVVMSSLAVLY
                     LLLGAEFLAAAQVIVYSGAIMVLFTFVIMLLNAGKEEPTHGSKLAYIAGFPGAAALLG
                     LLAFIFISRREAFGQARLGDYIATTGDLSQVLFTKLLLPFEVTSVLILIAILGAVALA
                     RKEH"
     gene            complement(1866..2927)
                     /gene="nuoH"
                     /locus_tag="VHE33_04205"
     CDS             complement(1866..2927)
                     /gene="nuoH"
                     /locus_tag="VHE33_04205"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HVT96684.1"
                     /translation="MDTSLIPFLLFNLAKVIVVVVLFLMCVAYTVLLERKLVGRIQNR
                     WGPSRVGPFGLLQPLVDGAKLFLKEDLTPEAVYRPLYILAPMIALGCSLLSISVIPFG
                     ADIRWHGMQFFQISDNNIGLLILLGITSVGVYGIALSGWSSNNKYSLLGALRASAQVI
                     SYELALGLSLVGIVIRSGSLRLRDIVGVQAPHGLLSWNFFGGFQFVAFFIYLMAAYAE
                     TNRSPFDLPEAESELTAGYHTEYSSMKFAMFFMAEYGNMITVGCVATLLFFGGWTSPF
                     GSYFHFGTGPIATAFFSLFWFVAKVFGFLFLYVWVRGTLPRFRYDQLMGFGWKFLVPV
                     AVANIVVTSFCIGLWMPVQ"
     gene            complement(2940..5273)
                     /gene="nuoG"
                     /locus_tag="VHE33_04210"
     CDS             complement(2940..5273)
                     /gene="nuoG"
                     /locus_tag="VHE33_04210"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HVT96685.1"
                     /translation="MADVTFTVDGKKLTAPAGTLLIEACRAHGIEIPAFCYYPGLSLQ
                     AACRMCVVRLEKVPKLQTACTTPVAEGMVVTTESPEIAQARKATIELLLGNHPLDCPV
                     CDAGGECELQDMTFKYGAHESKFVEIKQHREEQQWSPVVFFDRPRCILCYRCVRVCGE
                     GMDVWALGIQNRGAGSVIAPNRQDHLDCEECGMCIDICPVGALTSGAYRYKTRPWEMS
                     HVGTICTHCGDGCKTTLGVRRASDGMEIVRGDNRDKSGINGDFLCIKGRYAFDFANRE
                     NRLTKPMIRKSSGRLEEVSWEEAIEFAGNKLREIRDSKGGSAIGVIGSNRTTNEESYL
                     LQKFARTVLGTNNIDHHRTADFPAFAAALNNKGEASLRDIASAKAILLLGNDPTEQHP
                     GLAWQIRTNVRLNQARLYVANHNPIKLRRQATAFLEIPEDGYAGLVAWLGGTAAPLDD
                     DKAAVAFREALRKEESLVIVFGSEFRSRDVEALLAWGTTLSNVKFACLGDYVNSRGAA
                     DMGLYPDLLPGYLPVTASGPFASEFPTLPTAAGLNLLQMFDAAEAGSLAALYIVGANP
                     VARYARHNDALHNTFIIVQDMFLTETAQFADVILPAANLYEKTGTVTNTFGDVQLVKK
                     AADRAGVRTDFELIVRIAAAMGADIKKLVPFGTGTVRADMGQARGAQSGEADRHGVWL
                     AAHHLELKLSPFDPFAIFDEIQRLVPSYDVPRLDLLAGNDQHLKPALVQIENLAARPD
                     LVLPSNDTLFTSGSLGFYSKTLHSVLESHKPVETAAD"
BASE COUNT         1207 a         1476 c         1793 g          935 t
ORIGIN      
        1 taggcagcca cgccgcagcc caggaacatg tatccaagct gcgagatggt ggagtaggcg
       61 aggacgcgct tgatgtcggt ctgcagaatt ccgatggtag cggcaaagaa ggcggtggcg
      121 acgccgatcg tcgcgacgac ggccagcgcg aacggcgacc ggtcgaagat cacgtgggtg
      181 cgcgcgacca tgtaaacacc cgcggtgacc atggtggcag cgtggatgag cgcggagacg
      241 ggcgtcggcc cttccatggc atccggcagc cagacgtaga gcggaatttg tgcagatttt
      301 cccgtcgcac cgaggacgag gaagagcgcg atggcggtga ggaatccact ctgccattcg
      361 ggatgcgcac cgatctggct aaagacatca ctgaagctca gcgagccgaa gtgagcgatg
      421 agcaggaaca tcgcgatcag gaaaccgaaa tcgccgatgc gattgacgat gaaggccttt
      481 ttcccggcat tcgcagcgga gtcgcgcgtg aaatagaagc cgatgagcag atatgaggcg
      541 aggccaacgc cctcccagcc aacgaacatg aggaggaagt tttcggccag cacgagcgtg
      601 agcatgaaga agaggaagag gttcatgtag gcgaagaatc gccagtagcc ttcttcctca
      661 gccatgtatc ccaccgagta gacgtgaatc aggaagccga cgcctgttac cacgcagagc
      721 atgaccagcg tgagctggtc gagctggaag gcgaaaccgg cgtggaaaga gcccgaaacg
      781 atccaggtac cgagctgctc gatgtgcggc agagtcagcg agctgaattt tgtaacgatg
      841 cctgcgacct gaatggcggg gaccagcgag gcaatgagtg caatcgtcgt gacgagcgcc
      901 ttcggcagtt tgcggccgag aagaccattc accaggaatc cggcgaaggg gacgagcgga
      961 atgagccaga ggtgaaggtc gctgttcata gtttcatcag gtcgatctgg tcgacgttga
     1021 gggtgttgcg ggcgcggaag acggcaatga taatggcgag gccgacggcg gcctccgcag
     1081 cggcgaccac catgacgaag aagacgaaga tctggccgtt gacctggaga tgaccagtgg
     1141 tctgactcca ttgacgcgag aaggcgacga aggccagatt gaccgcgttg agcatgagct
     1201 caatcgacat aaagacggtg atgatgttgc gcttgatcag gaacgccgcg acgccgatgg
     1261 aaaagaggat cgcactgagg acgaggtaat acgcgatcgg gaccatgact cagtgctcct
     1321 ttctagcgag cgctactgcg ccgaggatag cgatgaggat caggacggag gtgacttcga
     1381 agggcagcag cagttttgtg aacaggacct ggctcaggtc gccggtggtg gcaatatagt
     1441 cgccgagcct cgcctggccg aaggcttcgc ggcgggaaat aaagatgaaa gcgagcagcc
     1501 cgaggagtgc ggcagcgccg gggaatccag cgatgtaggc aagcttgctg ccgtgggtgg
     1561 gttcttcttt gccagcgttc agcagcatga tcacgaaggt gaacagcacc atgatggcgc
     1621 cggagtagac gatcacctga gcggcggcga gaaattccgc accgagcaga agatagagca
     1681 cagccagcga gctcatgacc acgatgagcg agagcgcgct gttaatcggg tggcgctgaa
     1741 gcaggaggtt cagcgccccc gccacgcaca gcaccgcaaa gaagatgaag agagccagat
     1801 gcatgatctt ttaacttgcc cgcattctgc ggggactgcg cagcctgtcc aaaagcacga
     1861 ttgtattact gcacgggcat ccagaggccg atgcagaagc tggtaacaac aatgttggcc
     1921 accgcaacag gcacaaggaa tttccatcca aagcccatga gctggtcata acggaagcgc
     1981 ggcagcgtgc cccgcaccca gacatagaga aagagaaagc cgaaaacttt agcaacgaac
     2041 caaaacaggg agaagaaggc ggtcgcgatg ggtccggtac cgaagtggaa gtaactgcca
     2101 aaggggctgg tccagccacc gaagaagagc agcgtcgcga cgcagccgac cgtgatcatg
     2161 ttgccgtact cggccatgaa gaacatggcg aacttcatcg aactgtactc ggtgtgatag
     2221 ccggcggtca gttcggactc ggcttcgggc agatcgaagg gcgagcggtt cgtctctgcg
     2281 taggcggcca tcaaatagat gaagaacgcg acgaactgga atcctccgaa gaagttccag
     2341 gacagcagcc cgtgcggggc ctgaacgccg acgatgtcgc gcagacggag cgagccggag
     2401 cgaatgacga tgccaaccag ggagagtccg agcgccagtt cgtagctgat gacctgggct
     2461 gacgcgcgca acgcacccag aagcgagtat ttattgttcg acgaccagcc ggacagagcg
     2521 atgccgtaaa cgcctactga agtaataccg agcaggatca acaggccgat gttgttgtcg
     2581 gagatctgga aaaactgcat gccgtgccag cgaatgtcgg ccccgaatgg aatgaccgag
     2641 atcgacagca acgagcaacc gagcgcgatc atgggcgcga ggatgtacag agggcggtag
     2701 accgcctccg gcgtcagatc ttctttcagg aacagcttcg cgccatcgac cagcggctgc
     2761 agcagcccga atggtccgac gcggctcggt ccccaccgat tttggatacg gcccacgagc
     2821 ttgcgctcga gcagcaccgt ataggccacg cacatcagga acaacaccac caccacgatg
     2881 accttggcga ggttgaacag cagaaacgga atcaggctgg tgtccattgg cttttcttct
     2941 tagtccgctg ctgtttcgac gggtttgtgg gattcgagca cagagtgcag agttttgctg
     3001 taaaaaccga gcgagccgga ggtgaagagg gtgtcgttgg acggcagcac gaggtcggga
     3061 cgagcggcga ggttctcgat ctgcaccagt gccggtttaa ggtgctgatc gttgccggca
     3121 agcagatcca ggcgtggaac gtcgtacgag ggaacgagac gctgaatctc gtcgaagatg
     3181 gcgaacggat cgaaggggct gagtttgagc tcgaggtgat gcgcggcgag ccagacgcca
     3241 tgacggtcgg cttcgccgga ctgcgcgcca cgtgcctggc ccatatcggc acgaacggtg
     3301 ccggtgccaa agggaacgag ctttttgatg tctgcgccca tggcagcagc gatgcggaca
     3361 atcagctcga agtccgtgcg gacgccggca cgatcggcgg ctttcttgac cagttgtaca
     3421 tcgccaaagg tgttcgtgac ggtgccggtc ttctcataga gattcgcggc aggaaggatt
     3481 acgtcggcga actgcgccgt ctcggtgagg aacatgtcct gcacgatgat gaaagtgtta
     3541 tgcagggcgt cgttgtggcg cgcgtagcga gcgacgggat tggcgccaac gatatagaga
     3601 gcggcgagtg agcccgcttc ggcggcatcg aacatctgga gcaaattgag gccggctgcc
     3661 gtgggaaggg tggggaattc ggaggcaaag ggccccgagg ccgtaaccgg caggtagccg
     3721 ggaagcagat cggggtagag tcccatatcc gccgccccgc gcgaattgac atagtcgccg
     3781 aggcaggcga acttcacgtt ggataacgtc gttccccagg ccagcagcgc ttcgacatcg
     3841 cggctgcgaa actcggagcc gaagacgatg accagcgatt cctccttgcg cagtgcttcg
     3901 cggaaggcaa ccgcagcctt gtcgtcgtcg agcggggccg cggtaccacc cagccacgca
     3961 accagcccgg catagccatc ttccggaatc tcgaggaaag ccgtggcctg acggcgcagc
     4021 ttgatcgggt tgtgattcgc gacgtagagg cgcgcctggt tcaggcgaac attggtgcga
     4081 atctgccagg cgagaccggg atgctgctcg gtgggatcgt taccgagcag cagaatggct
     4141 ttggcgctgg caatgtcgcg cagcgaggct tcgcctttgt tgttgagcgc cgcagcgaag
     4201 gccgggaagt cggcggtgcg gtgatggtca atgttgttgg tgccgagcac agtccttgcg
     4261 aacttctgca ggaggtacga ctcctcgttg gtcgtccggt tggaaccgat gactccgatg
     4321 gccgaaccgc ccttgctgtc gcgaatctca cgcagcttgt tgccggcaaa ctcgatggcc
     4381 tcttcccagc tgacttcttc gaggcggccg ctcgatttgc ggatcatcgg cttcgtcagc
     4441 cggttctcgc gattcgcaaa gtcaaatgcg taccggccct tgatacataa gaagtcgccg
     4501 ttgatgccgg acttgtcgcg attgtcacca cgaacgattt ccataccatc gctggcacgg
     4561 cgaacgccga gagtcgtctt gcagccgtcc ccgcagtggg tgcagatggt gccgacgtgc
     4621 gacatctccc agggccgggt cttgtagcga taggcgccgg aggtcagagc gccgacggga
     4681 cagatgtcga tgcacatccc acactcttcg caatcgaggt gatcctggcg gttaggcgcg
     4741 atgaccgagc cggcgccgcg attctggatg ccgagggccc agacgtccat gccttcgccg
     4801 cagacgcgga cgcagcgata gcagagaatg cagcgggggc gatcgaaaaa gacgaccggc
     4861 gaccactgct gctcttcgcg gtgctgcttg atctcgacga atttcgattc gtgcgcgcca
     4921 tacttgaacg tcatgtcctg cagctcgcac tcgcccccgg cgtcgcagac cgggcagtcg
     4981 aggggatggt tgccgagcag cagttcaatg gtggctttgc gggcctgcgc gatctcagga
     5041 ctctcggtcg tgaccaccat gccttcagcg accggcgtcg tgcacgcggt ctgcagcttc
     5101 ggcaccttct cgaggcgcac gacgcacatc cggcaggcgg cctggaggga gaggccggga
     5161 tagtagcaga aggcaggaat ctcgatgcca tgagcacggc acgcttcaat gagcagcgtg
     5221 cccgcaggcg cggtgagctt cttgccatcg acggtaaagg taacgtcagc cattccgatc
     5281 cttcagacca gtgcgggctc aggttctgcc gcgtggtggt agggacagcc tttgccgtcc
     5341 aggtgatcct caaactcttt gcggaacttc ttcacgaagc cgatggtggg catcgcggcc
     5401 gcgtcgccga g
//
LOCUS       DASZYN010000166         3039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_7148, whole genome shotgun sequence.
ACCESSION   DASZYN010000166 DASZYN010000000
VERSION     DASZYN010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7148"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1601)
                     /locus_tag="VHE33_04215"
     CDS             complement(<1..1601)
                     /locus_tag="VHE33_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96686.1"
                     /translation="MFVPIARKPLLCVLCLAVVSGPAFAQRRHRGGPNEFGFRFLGPF
                     HGNRVAAVAGIPGDPSTWYAGAASGGIWKSTDGGNEWQPIFDQEPVAAIGALAIAPTD
                     HNVVWAGTGEAWAIRDIDVTGNGVYKSTDAGRTWQHMGLDETGRIGRVVIDPQNSDDV
                     YVCALGRLGGPQKERGVFRTSDGGKTWEHVLFVDENTGCSGLAMDPQNPRTIFAGMWQ
                     VSMHTWAELSGGPGSGVFVSHDRGATWTRIEGHGLPHSPVGKIDIAVAPTDSTRVYAL
                     IETDKQGSLWRSNDGGTDWELMSRDRTLIGRAGYYVRIAVSPADEDKVLVSNSSFLVS
                     TDGGRTFEDQPWGGDNHDIWIDPTNANRFAISFDGGLAITTVGGRGFNDVSLPIGQMY
                     HVAVDDQIPYYVYGNMQDNSTMRGPSIPLNEGGFEAGWDHGMGGCESGFTLPDPSDPN
                     IVWASCYGDEVTRWDALTHQARSVSPWMHTLDSAPNQAKYRCHWTPPLAIDPFDNNTI
                     YYGCQVVFKTSNGGQSWSVISPDLST"
     gene            complement(1727..>3039)
                     /locus_tag="VHE33_04220"
     CDS             complement(1727..>3039)
                     /locus_tag="VHE33_04220"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HVT96687.1"
                     /translation="GVAVGDYNGDGLPDLYVTQYPRSILYRNNGDGTFTDVTAKSGLA
                     APGWATSAVWFDYDNDGKLDLFVCRFADFNKEKNVWCGGHGPGERYYCKPNVYQPVPS
                     WLFHNNGDGTFTDVSKESGIATALAKGWGVVAADLNNDGRMDLFIGSDTAPNPLFINR
                     GGGRFEENGFLAGVAYNPFGVARSGMGVDAADYDQDGWLDLIVDNVDHEMYSLYHNGR
                     QGNFDDVSLRTPIGPATELMSGWGLKFFDFDNDGDLDLIVANGHPDLVIQQHHPELRY
                     LQPLLLFEQKAGLWKNVSGAAGPAFAQPLAGRGLALGDFDNDGSVDVLVTTNDGAPLL
                     LRNNAARKHHWLGLRLIGTRSNIDAIGAKVTWKSGDFARHRSKVGGGSYLSSHDPRMV
                     LGLGMRSEIDWLEVEWPAPGGRQRFTGLPIDRYITITEGKSDWK"
BASE COUNT          611 a          899 c          919 g          610 t
ORIGIN      
        1 gtcgaaagat cgggactgat gacggaccag ctctgaccgc cgttgctggt cttgaagaca
       61 acctggcagc cgtagtagat cgtgttgttg tcgaacggat cgatggccag gggcggcgtc
      121 cagtggcagc gatacttcgc ctggttgggc gcggagtcga gcgtgtgcat ccaggggctc
      181 accgagcgcg cctggtgggt cagcgcgtcc catcgtgtga cttcgtcgcc ataacaggaa
      241 gcccagacga tattgggatc gctgggatcg ggaagcgtga agccggactc gcagccgccc
      301 ataccgtggt cccatccggc ctcaaaccct ccttcattga ggggaatgct cggcccgcgc
      361 atggtgctgt tgtcctgcat gttgccgtag acgtagtaag gaatctggtc atcgacagcg
      421 acgtggtaca tctgcccaat gggcagactg acgtcattga agccgcggcc cccaacagtc
      481 gtaatcgcca ggccgccgtc gaacgagatg gcgaagcggt tcgcgttcgt gggatcgatc
      541 cagatgtcat ggttatcgcc accccacggt tgatcttcaa atgtgcggcc accgtcggtg
      601 gagacaagaa agctgctgtt ggagaccaga actttgtctt catccgcagg cgacaccgcg
      661 atgcggacgt aatagccggc gcgcccaatg agggtgcggt cccggctcat gagttcccag
      721 tctgttccgc catcattcga gcgccacagc gatccctgtt tgtcagtttc gatcaaagcg
      781 tacacacgag ttgagtcagt gggggccacc gcgatgtcga tcttgccgac cggagagtgc
      841 ggtaatccgt ggccttcgat gcgtgtccag gttgcgccac gatcgtgcga tacaaagacc
      901 ccgcttccgg ggcctccact tagctcggcc caggtgtgca ttgagacctg ccacatgccg
      961 gcgaagatcg tgcgcggatt ctgcggatcc atggcgagtc cggagcagcc tgtattctca
     1021 tcgacgaaga gtacgtgctc ccaggtctta ccgccatcgc tggtccgaaa gaccccgcgt
     1081 tccttttggg gacccccaag acgtccgagc gcacacacgt agacgtcgtc ggagttttgg
     1141 ggatcgatca caacgcgtcc gatgcggccg gtttcgtcga gtcccatgtg ttgccaggtc
     1201 ctgcctgcgt cggtcgactt gtagacgccg ttcccggtga cgtcgatatc gcgaatcgcc
     1261 catgcctctc cggtcccggc ccagacgaca ttgtgatccg tgggtgcaat cgcgagggct
     1321 ccgattgcag cgacgggctc ctgatcgaag atcggttgcc attcgtttcc gccgtcggtg
     1381 gacttccaga tgccgccgga tgccgcacct gcataccatg tgctgggatc cccagggatt
     1441 ccggccactg ccgcgacacg gtttccgtga aaaggtccga gaaaacgaaa gccaaactcg
     1501 ttggggccgc cacggtgccg gcgctgtgcg aaggcggggc cagaaaccac agcaagacaa
     1561 aggacgcaca gcaggggttt gcgcgcaatc ggtacaaaca ttttcgcttt gctccagggc
     1621 ggacagactg ggaagaagga ttgtacacta tatgaaatgc aattcgactg aggcgctact
     1681 ctgcagacag agtggttttg agtgttgtgc cagtcaacct gctggactac ttccagtccg
     1741 acttgccttc agtgatggtg atgtaacgat cgatgggcag accggtgaag cgctgcctgc
     1801 cgcctggcgc cggccattcc acttccagcc agtcgatctc tgaccgcata ccaagaccaa
     1861 gcaccatgcg cggatcgtgc gaagaaagat agcttccgcc gcctacctta ctgcgatggc
     1921 gtgcaaagtc accgctcttc cacgtcactt tcgcgccgat tgcatcaata ttggatcgcg
     1981 tgccgatcag gcgcaggccc agccaatggt gtttacgcgc ggcgttgttc ctgagcaaca
     2041 gaggtgcacc atcgttggtg gtcacaagca catcgacaga gccgtcgttg tcgaaatccc
     2101 caagagcgag cccacggccg gcgagaggtt gcgcgaacgc gggcccggca gcaccgctga
     2161 cattcttcca gaggccagct ttttgctcga agaggagcag aggctgcagg tagcgaagct
     2221 ccgggtggtg ctgctggatc accagatccg gatgaccatt ggcgacgatc aggtccaggt
     2281 cgccatcatt gtcgaaatcg aagaacttca ggccccatcc actcattagc tcggtcgcgg
     2341 gtccaatggg agtacgcaaa gaaacgtcat caaaattgcc ttgacggcca ttgtggtaga
     2401 gcgagtacat ctcgtgatcg acattgtcga cgatgagatc gagccagccg tcctggtcgt
     2461 agtcggcagc atcgacgccc atgccggaac gggcaacgcc gaagggattg taggcgactc
     2521 cggccaggaa tccgttctct tcgaagcggc cgccgcctcg attgatgaac aagggattgg
     2581 gcgcagtatc gctgccgata aacaagtcca tgcggccgtc gttattgaga tcggccgcca
     2641 ccacgcccca gcctttcgcc agcgcggttg cgatccccga ttccttactc acatcggtga
     2701 acgtgccatc gccattgtta tggaaaagcc agctgggcac cggttggtag acgttcggct
     2761 tacagtagta ccgctcgccc gggccatggc cgccgcacca gacgttcttt tctttgttga
     2821 aatcggcaaa gcggcaaaca aagagatcga gcttgccatc gttgtcatag tcgaaccaca
     2881 ctgcgctggt cgcccacccg ggagccgcca gcccggattt cgcggtaacg tcggtgaagg
     2941 tgccgtcgcc gttgttgcga tacagaatgc tgcgcggata ttgcgtgacg taaaggtccg
     3001 gcagaccgtc gccgttgtaa tcgccgaccg cgacgccca
//
LOCUS       DASZYN010000167         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_647312, whole genome shotgun sequence.
ACCESSION   DASZYN010000167 DASZYN010000000
VERSION     DASZYN010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647312"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VHE33_04225"
     CDS             complement(<1..671)
                     /locus_tag="VHE33_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HVT96688.1"
                     /translation="MSIAARLLVCAATVFTAPFLLHADNISKIEDRMRSLRSLSETQR
                     PATTVQIAQDIRALPPGTQKLKLADALAHLVTEGDQGQDTIQAVADTLRQSLSETPVA
                     PKKSETPMPYMDLATLVRYEGAKEELNDPLYAKAQQTLADNEAQIAKADFTLKDLKGK
                     KWTLSELRGKLVLVNFWATWCGPCRVEMPYLDALYTRFQSQGLVVLSITDEDAFKVSQ
                     FISSS"
     gene            complement(749..>2384)
                     /locus_tag="VHE33_04230"
     CDS             complement(749..>2384)
                     /locus_tag="VHE33_04230"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HVT96689.1"
                     /translation="DDHPRSASAVLAELPASPARGTLRPIASLPGALLPNAPTPNLPG
                     APVSNISDVRESSSVVAVRPPQGPVLHEDCSIDTNHSPRCRVHWHQLILSSAVFNAFQ
                     NGGNLYTGYWYRWETSHGKWWDRYVNSVEEWHWNSWADQNPILDDYVGHPMMGAITND
                     LWIQDDPKSMTVTQSNTWPYWRRMLRAGAFSTFYSFEWKMGPLGEAGIGHNGDHIIHS
                     QTGANPTTETGWVELVTTPVGGALWTMAEDALDKHVVAPAEGQGRNPFGMLALSFLEP
                     AHATANIFRWRPPWYRDDRVAKAKSFWSDPPGEYEVRFAKSRPGISGSENEAAERPIP
                     AVSSVVPDASPAIVRNPTAHDAPVWPNPGGVHEFGAWWGLSLISGHIWGYAGDIKYMP
                     VDVRYTYLFTAHRDWTMRYAPEVTALAMLDQPNPQAAPNDRLHQRKRIYGSGVSPVGF
                     ESDFFPAKRVQPFLTTNGGFLYFDDRVLSPQGSQWMYTIDFGTGIHVFRKARQDWTIG
                     YRYQHLSNANISHHNPGTDTSVFYIGVSRFRTKGHR"
BASE COUNT          447 a          666 c          798 g          473 t
ORIGIN      
        1 ttcgacgatg agatgaactg ggaaaccttg aaggcatctt catcggtgat ggagagtacg
       61 acgagcccct gagactggaa gcgcgtatag agcgcgtcga ggtagggcat ctcgacccgg
      121 cacggcccac accacgtcgc ccagaaattg acgaggacga gcttgccgcg gagttcggac
      181 agagtccatt tcttcccttt cagatccttc agggtgaagt ccgccttcgc gatttgcgct
      241 tcgttgtcgg ccagcgtctg ctgcgctttg gcgtagaggg gatcgttcag ttcctctttt
      301 gcgccttcgt agcggacgag ggtggcgagg tccatgtagg gcatgggcgt ttcagatttt
      361 ttcggcgcaa caggcgtctc gctcaacgac tgccgcaagg tatcggccac ggcctgaatc
      421 gtgtcctggc cctgatcgcc ttcggtcacc agatgcgcca gtgcatcggc aagcttcagc
      481 ttctgggttc cgggcgggag agcacgaatg tcctgcgcga tctggaccgt cgttgccggc
      541 ctttgagttt cagacaaaga gcgcagcgag cgcatgcggt cttcaatttt ggagatgttg
      601 tcagcgtgca gaagaaacgg tgcggtgaag acagttgcgg cgcacaccag cagccgcgca
      661 gcgatggaca tgatcgagcc tctggaccgg aaagatgggc accatcatac tccaggtgat
      721 tcccggcgcc ggtgaggcgg ctcgctcctc aacgatgccc tttggtacgg aagcgcgaga
      781 cgccgatgta gaagacgctg gtgtcggtac cgggattgtg gtggctgatg ttggcgttgg
      841 agaggtgctg gtagcggtag ccgatggtcc agtcctggcg cgccttgcgg aagacgtgga
      901 ttccggttcc gaaatcgatg gtgtacatcc actgcgatcc ctggggggag aggacgcggt
      961 cgtcgaaata aagaaaaccg ccattggtgg taaggaaggg ttgcacccgt ttcgcgggga
     1021 agaagtcgga ttcgaatccc accggactaa cgccgctgcc gtaaatgcgc ttgcgctgat
     1081 gtagacgatc gttcggggcg gcctgcgggt tcggctggtc cagcatggcc agcgcggtca
     1141 cttcgggagc gtagcgcatg gtccagtcgc ggtgcgcggt gaacagatag gtgtagcgga
     1201 cgtcaacggg catgtacttg atgtcgcccg cgtatcccca gatatgtccg ctgatgaggg
     1261 acagccccca ccaggcgccg aattcgtgca cgccgccggg attgggccag accggagcat
     1321 catgcgccgt cgggttgcgg acgatggcgg gcgaggcatc gggaaccact gaagaaacag
     1381 ccggaatggg tctctccgct gcttcattct ctgacccgct tatgccgggc ctggactttg
     1441 caaagcggac ttcgtactcg cccggtggat cgctccagaa ggactttgct ttcgccacgc
     1501 gatcgtcgcg ataccagggt ggccgccagc ggaagatatt ggctgtggcg tgagccggct
     1561 cgagaaaact gagcgccagc atgccgaagg gattgcgacc ctggccttcg gcaggcgcga
     1621 ccacgtgctt gtccagagca tcttcggcca tggtccacaa ggcgcctccc accggggttg
     1681 tgaccagttc gacccagccg gtctcggtgg ttggattggc gcccgtctgc gaatggataa
     1741 tgtgatcgcc gttgtggccg atgccggctt cgcccagagg acccatcttc cactcaaagc
     1801 tgtagaaggt ggagaacgcg cccgcccgca gcatgcgccg ccagtagggc caggtattcg
     1861 actgcgtcac cgtcatgctc ttcgggtcgt cctggatcca aagatcattg gtgatcgcac
     1921 ccatcatggg gtggcccaca tagtcatcga gaatggggtt ctggtccgcc caggagttcc
     1981 agtgccattc ttcgacggag ttcacgtagc ggtcccacca tttgccgtgc gacgtctccc
     2041 accggtacca gtagccggtg tagaggttgc cgccattctg aaaagcgttg aagactgccg
     2101 aggaaaggat cagctgatgc caatgcacgc ggcatcgcgg cgagtggttg gtatcgatgg
     2161 agcagtcttc gtggaggaca gggccctgcg gcgggcgcac cgcgaccacg ctgctggatt
     2221 ctctgacatc ggaaatattc gataccggcg caccgggcaa gttgggcgtg ggcgcattcg
     2281 gcagcaatgc accgggcagc gacgcaatgg gcctgagggt accgcgcgcg ggactggcgg
     2341 gcagttcggc aagcacggcg gaagcagatc gtgggtgatc gtcc
//
LOCUS       DASZYN010000168        19055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_774720, whole genome shotgun sequence.
ACCESSION   DASZYN010000168 DASZYN010000000
VERSION     DASZYN010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 19055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19055
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774720"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VHE33_04235"
     CDS             complement(<1..835)
                     /locus_tag="VHE33_04235"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HVT96690.1"
                     /translation="MPSSKPDSTNTGKWSGSAIREMFLKFFETKGHRRVHSSSLVPAN
                     DPTLLFTNAGMTQFKDVFLGLERRDYARATTSQKCVRAGGKHNDLENVGFTRRHHTFF
                     EMLGNFSFGDYFKKDAIAYAWELVTSPEWFGIPKDKLYVTIFEGDAEVPRDDEAERLW
                     MDVGVPKERIFTYGRKDNFWQMGETGPCGPCSEIFYDMGMEAAETPGVDRPFGEDEAR
                     YVEIWNLVFMQFDRAAVKDAKGAVTEYTLTPLPKPSIDTGMGLERTAAVLQGVVSNFE
                     TD"
     gene            complement(990..1775)
                     /locus_tag="VHE33_04240"
     CDS             complement(990..1775)
                     /locus_tag="VHE33_04240"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HVT96691.1"
                     /translation="MNRHVQPEDSDLYALGALDGEEKQALEAHLRLCADCAREVDAAR
                     RTAALLGLAAPAAADPPARVKEELLRRVRSERGAVLVPQPERRRGFAFGWLTPAFGVA
                     TVFFAALAAVLWMRDVRDMRRIHALEGQLAEVQTRSQEIARASKTTDKLLEMPGTRRV
                     ALMPMPGMGNGHGGLLYNSKMGMVACTGWLPTPPKGKSYQLWLVPMEGKPMPLYTFGE
                     EWTEPMEMHVTPGMQAKAFAVTEEPAGGMPWPTGPKVLEGVGE"
     gene            complement(1744..2319)
                     /locus_tag="VHE33_04245"
     CDS             complement(1744..2319)
                     /locus_tag="VHE33_04245"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVT96692.1"
                     /translation="MTQPDSALVERIMAGDEGALSALYDRYSGMLYAMLLRILKDAGA
                     AEEVLQDLFLQLWRGAALFDASRGSLAGWLLVIGRNRALSRLRGKQRREFVPDDIEGF
                     SLDAVPSSEDLENEAARMQLMQRLRGAMAALPAEQREAVELAYFEGMTQTEIAARTGS
                     PLGTVKSRVRAAMQSLKQLFDDESTRTTRRF"
     gene            2597..3517
                     /locus_tag="VHE33_04250"
     CDS             2597..3517
                     /locus_tag="VHE33_04250"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HVT96693.1"
                     /translation="MSQKEAAGTLSAEQLLNRVRLERALNRRNFLTRLGVTGAAVAGG
                     ALLAGCGGSGHHSVMAAGPSESDVLNFALNLEYLEATFYSYVVTGGDIPSSSTGGGPA
                     PTGAPGATPAFQSQLLADMFAEIYYDEISHVNDLRTALGNAAVARPQLNLAALGTVST
                     ANYITFARLFEDVGVTAYAGASTSLTGTNLQYAAQILAVEGFHAGAIRLAAIEQGVPY
                     LPSAVIPADMEDVPPLDPGSVTAAESGPTTQGAFFATAGANGVNKTLPGLAYTRTTSQ
                     VLAIVYANTATGTAKGGFFPNGLNGSIKTV"
     gene            3725..4066
                     /locus_tag="VHE33_04255"
     CDS             3725..4066
                     /locus_tag="VHE33_04255"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HVT96694.1"
                     /translation="MNEFVFLYRGGEQGRSPERAQQMMQKWMAWFKQLGEKGHVKDPG
                     QPLERAGKLVSGRRKTVTDGPFAEAKDVVGGYTLILAKDLDEAAELSKGCPIFEVEGQ
                     VEVRPVMKMNM"
     gene            4066..5313
                     /locus_tag="VHE33_04260"
     CDS             4066..5313
                     /locus_tag="VHE33_04260"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HVT96695.1"
                     /translation="MELSEHLFRHEAGRMVATLTRIFGVHNLGLAEDVVQEAFCRALE
                     IWKFRGVPENPSAWLMLTAKHCALDALRRERTARTFAPELTRQLESEWTLAPTVEEAF
                     GPAAIEDDELRMMFSCCSPHLAEEAQVPLILNILCGFSVDEIASAFVSSHAAIEKRIT
                     RSKKVLARSRKLFDVSGAADFARRLPAVQRALYLLFNEGYHGASPESAVRADLCRESM
                     RLAAMLLKNPLGARPSTYALAALMHLNAARLPARLNGSGHLTALADQDRSLFDRELTR
                     EGQRLLEQSAAGDEVSDYHFEAAIAWLHTSAERTEDTDWGAIIGFYDQLMAVRPSPVV
                     ALNRAIAIGQQEGPERGLEAIRAIEDAGRLARYPFYHAAMGELELRSRKKEAARGHFA
                     VALELARNAMERDFFEGRMGECG"
     gene            complement(5404..6552)
                     /locus_tag="VHE33_04265"
     CDS             complement(5404..6552)
                     /locus_tag="VHE33_04265"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HVT96696.1"
                     /translation="MRWMAALAFVAAATSLQAVAQSPTEWPDITQAQDYTWHLASSLE
                     TTGANHDSRTITPGQTLTILDVDGPAVISHLWFTISSPEPYHLKRIVLRIYWDGEESP
                     SVETPIGDFFGLGLGEYFNWQSAMLQVGSARAMNCFFPMPFQHHARITITNEGKMELS
                     NLYYNIDYRTYTHPLPSSTLYFHAEYRQAQPNHGWTSQWYENGDPLINYKRNPDGKDN
                     YVWFDAKGRGQYIGVTMSVLQNQDGWWGEGDPMFFIDGQATPVTGTGSEDYFLGAWDF
                     GGAAFAYPLYGAPMVGRELAGERSAVYRFHLDSPITFTKSMKATIEHGHANARSDNFY
                     SVAYWYQTEPHQPFPPLPSMDDRIPSLEVTNGPGNESPAHPNAGLSPK"
     gene            6838..7539
                     /locus_tag="VHE33_04270"
     CDS             6838..7539
                     /locus_tag="VHE33_04270"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HVT96697.1"
                     /translation="MCGRYYRRSDKQRIAEAFKVGVPPSFEILPDYNVAPTTFQPVVV
                     EDRETGEREIRAMYWGLIPYWAEEPKKLGVSTINAKAESLLEKPMWREPFERRRCLVP
                     ADGFYEWQKFSEKTKQPWAIGLKDDGLLAFAGLWDRWRSKDKQTVLESFAIVTTEANE
                     LTAPMHDRMPVILKPADYERWLRREDAGRPPLDLLRPFDADRMRTWRVDAAVGNVKNN
                     SAALLDEVPAGTLFG"
     gene            complement(7564..10275)
                     /locus_tag="VHE33_04275"
     CDS             complement(7564..10275)
                     /locus_tag="VHE33_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HVT96698.1"
                     /translation="MAPSSRLFVPFAVICLALTAAAAFAQRNAVPDPIELNSNWQLQD
                     SAKVSDPGATVSSASYQPAGWLAATVPGTVLTSLVNDKVYPEPTYGENNRETIIPESL
                     NKTSYWYRTTIAIPKNYAHHQVWLHFDGANFTSEVWVNGTHAGTINGAFIRGRFDITP
                     LVRPGSTAVLAVKVSPQPHPGDPHEHTIANGVGKNGGITAIDGPTFLCTIGWDWLNAV
                     RDRDTGLWQKVWLSASGPVLINDPQVITDLPLPKTDSADITVNATLQNTSPKPQTGTL
                     EGTIGDITFSQKVTLKPNETQTVTFDPKTYAPLHLANPKLWWPNGYGPQNLYTLHLHF
                     VEHGHDSDAHSTTFGIRKITYEAPGMDALTISVNGVKVFIRGGDWGLDEALKRIPRAR
                     LDAEIHMHALANMNLIRNWVGQSTGDDFYELCDKYGILIWDEFFQPNPSDGPNPDDFA
                     IYMANVRDKILRYRNHPSIALWCARNEGYPPPEIDAALRKLIPELDPARLYQPSSTAG
                     RGVMSSGPYYWREPRAFYVFDAPFKTETGSMSVPTLESIHGMMPEKDWNTINDDWAAH
                     DFAKGAQHGDTYPYVLAGRYGRIANLADFVRKAQMMNYEAYRAMYEGRNKLLFKPATG
                     VITWMSNPAQPSFVWQLYHYDLEPNASLFAVKSASELVHIQMDEDDFHLRVINNLPTD
                     LSGDIAHIAIYTLWGKGQPALEKDIPVSGPADTAIDLGPIPLPEHLSSLYFAELELRD
                     AQGQILSRNLYWEPNPSQGDDLTPLNQLPTVKLEATVARHDSGSDSAARLGLTVTLKN
                     PGPSIALMAHLQLRRKSTSDRVLPVFYSDNYISLAPGETRTVTIDAALADLKGDTPLV
                     VVDGWNIDVTPVSNADSALALNTDAQVSHWPATGLPMITAGLEPTER"
     gene            11048..12250
                     /locus_tag="VHE33_04280"
     CDS             11048..12250
                     /locus_tag="VHE33_04280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT96699.1"
                     /translation="MRIAIVTETFLPKIDGIVTMVTKTVETLRAAGDEVMVFAPSGGP
                     AELFGAEVVSLPSIPFPFYPELNVAAPRASMRKHLERFRPDAFHLFEPSLLGIGGIYY
                     GKVLHIPIVISYHTNLPAYLHYYRLGFFEGPTWKLMRARHLRADLNLCTSTQMMEELH
                     HHDIDRLALWERAVDAQRFHPSKRSAEMRSLLSGGEPQKPLILYVGRLSAEKDVETLR
                     TLVEAMPETRLAIIGDGPVRKELEKHFEGTPTYFAGYMRGEALAQAYASADVFVMPSK
                     TETLGLVLMEAMAAGCPVVACRAGGVPDAVEDGVTGFLYEPDDPDGLVKKVAWVLEHT
                     AERTEVQVQARADVERHSWEGSTEQLRRYYAQAIEHPRPKGVAKGQMRWRGVTRRVVV
                     ASLRRLLP"
     gene            complement(12239..13063)
                     /locus_tag="VHE33_04285"
     CDS             complement(12239..13063)
                     /locus_tag="VHE33_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96700.1"
                     /translation="MRPTVRTATLAATLVTAATFASPAFLTAQNVNAAITNLSDPPVA
                     SRPAGNLALDDIVQRLANGEKKRAQDLKGYSELRTYSVSYHGFPANLSSTIVVAVTYS
                     APATKQFRIVSQTGSKMLADRVLKKLFAAEQESTADPRQTAPTTANYNFTLVGQQLLD
                     GRPCYLLRVEPHTNSKLLYRGTIWVDADDFAVAKIEAEPAHNPSFWIRGTKIHHVNSR
                     TGEFWLPEHNESDTDVRFGGHAVLTIDYGAYKMDSPAPAAAQASLQPNPAPLPSGK"
     gene            13264..14538
                     /locus_tag="VHE33_04290"
     CDS             13264..14538
                     /locus_tag="VHE33_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96701.1"
                     /translation="MNLERSRGYVGDDGGGVHNELLVLSRDQDRLSAWQSGVSLHSHT
                     RHSQESLGFLAAFFERRALTRRWYACQRESCRRVTGIDLNLERAHWTPPLCERMAQDV
                     EQRQIEQLGLRPMVSLTDHNSIEACMLLRQDAAFADAPISTEWTVPFGGAVFHFGIHN
                     LPARSAQDLMGAMHEATYLADEDRILRLFAELSAIEGVLVVFNHPLWNFYSIPADRFM
                     YELTRFLGMGNRYVHAFELNGMRSHAENRAALKLAAEWNQVVISGGDRHGREPNASLN
                     LTNAADFPEFVEEVRDGRQSMVLVMPQYAAPLGWRLYRNFTHVIGEYPEHPEGRRRWD
                     ERTYHPDRHGAVVPMRLLWKSGEPAEFLKAMFGAAMMAGRVPVDGLWRRLMRRENESL
                     QLPHRVEGAGYRVQVTGHRSQAVELASDGGEL"
     gene            complement(14512..16062)
                     /locus_tag="VHE33_04295"
     CDS             complement(14512..16062)
                     /locus_tag="VHE33_04295"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HVT96702.1"
                     /translation="MRAVRNFLCWMFALGAILFLRSSLQAILDIASWPEAHLPPTFLK
                     ASVHAATPLLGVIYAAACWTFWVGKPSARAWGLLASAANTLLGCLFLYMDRRFLTAPA
                     TAFLNPDALLLAVGLAGLIAFRRRDAVQAEQLMQPLPGDGTHALVNRLIWVAGTGGFI
                     SAMTWWWRWAESNGLVWIRSSFLHDFFLLLLLEVLMVALHEFGHAAAASALGMKLRAF
                     IVGPFQGRMRDGHWEFRFVLAGLLAIGGATAVAPKDPHRPLWRDIVMVASGPFFSLIG
                     GLAAVALTLGAPGQPWQAEWLPLAFFATLSLLSAVLNLIPFRTKAFYSDGARILQLLS
                     RGPWADLHRAFSAAAATTVSPLRPRDYDIDAILRAGSSISIGQQALHLRLLAASYYLD
                     CGRVSEASLALAEAETIYQESASDIPAELHTPFIFGKACLEHDPEGTRLWWERMQAKH
                     PTRHNADYWIARSAFLWSEDRIGEAREAWEKGNALVQQLPHTGACETDRDHFALLEKE
                     LQLAPVAG"
     gene            complement(16189..16605)
                     /locus_tag="VHE33_04300"
     CDS             complement(16189..16605)
                     /locus_tag="VHE33_04300"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVT96703.1"
                     /translation="MNRSPKSYDLALLFAALADRTRLRLLNLMDGREICVCYFVEILG
                     QSQPKISRHLAYLRRADVVAARREGKWMHYRIVVPPSSGAAKILRETLIVLRQDKAMQ
                     ADLNRLNKSCCVPLKTPALDGVPAPALIAPLATSSC"
     gene            16682..17548
                     /locus_tag="VHE33_04305"
     CDS             16682..17548
                     /locus_tag="VHE33_04305"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HVT96704.1"
                     /translation="MGSTTSVQEQVKAKYGSAARAVAEAGTVQACCDPGLRCCDPITA
                     NLYGAEQKGMVPEKAVRASLGCGNPTALAELRAGETVLDLGSGGGIDVLLSAQRVGPS
                     GKAYGLDMTDEMLALARANQEQAGVTNVEFLKGEIENIPLPDDSVDVIISNCVINLSA
                     DKDRVLREAFRVLKPGGRFAVSDVVVRGSVPDEVRRSMLLWVGCIAGALEEKEYGEKL
                     AAAGFVKTSMETTREYNVEDARQFLTEAGVDVDAIAPQVNGKFFSAFIRANKPQTACC
                     QPGCCTSAGARE"
     gene            17554..18045
                     /locus_tag="VHE33_04310"
     CDS             17554..18045
                     /locus_tag="VHE33_04310"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HVT96705.1"
                     /translation="MAGPYHVLFLCTGNSARSIMAEAILNHKGKGKFTAYSAGSHPTG
                     RPRPEALKQIESAGLSTAGLRSKSWDEFSGGGAPKLDFVFTVCDNAAKETCPYWPGQP
                     MTAHWGVADPAAVKGTPEEIGRAFREAFTVLDRRIGLFLALPLATLENMAIQREIDEI
                     GQR"
     gene            18344..19015
                     /locus_tag="VHE33_04315"
     CDS             18344..19015
                     /locus_tag="VHE33_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96706.1"
                     /translation="MYSYVANWQIPRSHWSEVAQGAAAYKTVADKALADGTIVGYGFD
                     EAVVHTADGDTHDDWWSSMSIAGLLKMRTQLFAAGTPTSPAMDAATKHWDEILMSRYY
                     NKKSGSYKNAYTWVAMYQLKPDAGDDAVEMLSRNVIAPFLEKLFADGTILEYEIDEQA
                     VHSQAPGTFSVVWVSPTAEGVDKVNAALFETVKAQPMIEPAFGSATKNSAHRDELLLS
                     EGAYK"
BASE COUNT         3454 a         4920 c         6991 g         3690 t
ORIGIN      
        1 gatccgtctc gaagttgctg acgacgccct gaagcaccgc ggcggtgcgc tccaaaccca
       61 tgcctgtatc gatggagggc ttgggcaaag gggtcaacgt gtattcggtc accgctccct
      121 tcgcatcctt caccgcggcg cggtcaaact gcataaaaac caagttccag atctcgacat
      181 accgcgcctc gtcttcccca aacggccggt ccacgcctgg agtctccgcc gcctccatgc
      241 ccatgtcgta aaaaatctct gagcatgggc cgcacggccc cgtctcgccc atctgccaga
      301 agttgtcctt gcgcccgtag gtgaaaatcc tttccttcgg gacgccgacg tccatccaca
      361 gccgctccgc ctcgtcgtcg cgcggcacct cggcatcgcc ctcgaaaatc gtcacgtaca
      421 gcttgtcctt cggaatccca aaccattccg gcgacgtcac cagctcccac gcgtatgcga
      481 tcgcgtcctt cttgaagtag tcgccgaagc tgaagttccc cagcatctcg aagaaggtgt
      541 ggtgccgccg cgtgaacccg acattctcca gatcgttgtg cttcccgccc gcccgcacac
      601 acttctgcga tgtcgtggcg cgcgcgtagt cgcgccgctc caggcccaga aacacatcct
      661 taaattgggt catccccgcg ttggtgaaca gcagcgtagg atcgttggcc ggcaccagcg
      721 acgacgagtg cacgcgccgg tggcccttcg tctcaaagaa cttcagaaac atctcgcgga
      781 tggccgaccc ggaccactta cccgtattgg ttgaatctgg cttactggaa ggcatacacc
      841 ccgagtttac cggagcagga ccggcccatt cctgaacaga ctgccaaggc tctgcccaga
      901 atgctggtcg attggcagca ccgcctttgg ctaacagcta aaagccagaa agggaactcc
      961 tgcgccctgc gacctgagcc ctctctttct cactccccca caccttccag cacctttggt
     1021 cccgtcggcc acggcatgcc gcccgccggc tcctccgtca ccgcgaaggc cttcgcctgc
     1081 atccccggcg tcacgtgcat ctccatcggc tcagtccact cctcgccgaa ggtatacaga
     1141 ggcatcggtt tgccctccat gggcaccagc cacagctgat agctcttgcc cttcggcgga
     1201 gtcggcagcc accccgtgca cgccaccatc cccatcttcg agttgtagag caagcccccg
     1261 tgcccgttgc ccatgcccgg catcggcatc agcgctaccc gcctggttcc gggcatctcc
     1321 agcaatttgt ccgtcgtctt cgacgcccgc gcaatctcct gcgaccgcgt ttgcacctcc
     1381 gccagctggc cctccagggc atgaattcgg cgcatatccc gaacatcgcg catccacaag
     1441 accgccgcca gcgctgcaaa gaaaaccgtc gccacgccaa acgcaggtgt gagccagcca
     1501 aatgcaaatc ctcgccgccg ctccggctgg ggaaccagta cggcaccccg ctccgaccgc
     1561 actctccgca gcaattcctc cttcactcgg gccggcggat ccgcagccgc cggcgcagcc
     1621 agtcccagca gcgctgctgt cctgcgcgcc gcatccacct cacgcgcgca gtcggcgcac
     1681 aggcgcaggt gggcttccag cgcttgtttt tcttccccgt cgagcgcgcc gagcgcgtac
     1741 aggtcagaat cttcgggttg tacgtgtcga ttcatcgtca aaaagctgct tcaaactctg
     1801 catcgcggcc cgtacccgcg acttcaccgt tcccagcgga ctcccggtcc gcgctgcaat
     1861 ctcggtctgg gtcatgcctt cgaaatacgc aagttccacg gcctccctct gttcggccgg
     1921 caatgcggcc atcgctccgc gcagccgctg catcaactgc atccgcgccg cctcgttctc
     1981 cagatcttct gaagacggca cagcatcgag cgaaaatcct tcaatatcgt ccggcacaaa
     2041 ctcccgccgc tgtttgccgc gcaggcggct gagcgcacga ttgcgcccta tcaccagcag
     2101 ccatcccgcc agcgaacccc ggctcgcgtc gaagagcgca gccccgcgcc acagctgcag
     2161 gaacagatcc tgcagcacct cttccgccgc gcccgcatcc ttcaggatgc gcagcagcat
     2221 ggcgtagagc attccggaat agcggtcgta gagcgcagac agcgctccct cgtctccggc
     2281 catgatccgc tcgacgagcg ccgagtccgg ctgagtcatg gcgcctcgct tcggctgctc
     2341 atgggtctac gaattagata cgcaaaattg caggtcaatg gattgcaagc cgtgatttat
     2401 cgtgacgctc cggaggcgct tagggtcgac tttacccgaa ttggctgtgc caggttctct
     2461 ctttgatgcc atgcatctcc caaatcctgc ttatctgcct cacaatccgc caaatctttt
     2521 taaatccgct ttgtttacca gaaagtcaat ccgcccttct cctggccgcg tactcatcac
     2581 aggaggaatt ccctatatgt cccaaaaaga agccgccggc acgttatcgg cggagcaact
     2641 cctcaatcgc gttcgccttg agcgcgctct caaccgtcgc aattttctca cccgtctggg
     2701 cgttaccggc gcggctgtgg ccggcggcgc tcttttggcc gggtgcggcg gatcgggcca
     2761 ccactccgtc atggccgccg gaccttccga atccgacgtg ctcaactttg ccctcaacct
     2821 tgaatacctg gaagctacgt tctatagcta tgtcgtgacc ggtggcgata ttcccagcag
     2881 ctccaccggc ggcggtcccg cacccaccgg tgctcccggc gccaccccgg cctttcagtc
     2941 gcaattactc gccgacatgt tcgccgagat ctattacgac gagatcagcc acgtgaacga
     3001 ccttcgcacg gccctgggca acgccgctgt tgcgcgcccg caactcaacc tcgccgccct
     3061 gggaaccgtg agtaccgcca actacatcac gttcgcccgc ctcttcgaag atgtaggtgt
     3121 caccgcctac gccggcgcct ccaccagcct gaccggaacc aatctccagt acgccgccca
     3181 aatccttgcc gttgaaggtt tccatgccgg tgcgattcgc cttgccgcta ttgaacaggg
     3241 cgtgccctac ctgccctctg ccgtcatccc tgcggatatg gaagacgtcc ctcccctcga
     3301 ccccggctcc gtgacagctg ccgaaagcgg accgacaacc cagggcgcct tttttgccac
     3361 ggctggcgcg aatggcgtca acaagacgct ccccggcctt gcctacaccc gaacgacctc
     3421 ccaggtgctg gccatcgtct acgcgaacac agccaccggg accgccaaag ggggattctt
     3481 ccccaatggc ctcaacggca gcatcaaaac tgtctgacaa agcctgcttc ctgaattaca
     3541 aacacccatt tctcaacggg ccggtgtact ctgccggccc ggctgttttt tgagtccgct
     3601 cgatccgatc gtccctgtat ggggcgggag gtcgcggtct cgaatcggtg gaaaattgtt
     3661 gtccggcgga gtggcgctca aacgtctatg ctccggaagt tcatttcaac aaggagaagt
     3721 ggtgatgaat gaatttgtgt ttctttatcg tggcggcgag cagggccggt cgccggagcg
     3781 tgcgcagcag atgatgcaga agtggatggc gtggttcaag caactgggcg agaagggaca
     3841 tgtgaaggac cccggccagc cgctggagcg cgccggcaaa ctggtgagcg gccgccgcaa
     3901 gacggtcacc gatggtccgt ttgcggaggc caaggacgtg gtgggcggct acactctgat
     3961 cctggcgaag gacctcgatg aggcggccga gttgtcgaag ggctgtccga tttttgaggt
     4021 tgaaggtcag gtggaagtgc ggccggtcat gaaaatgaat atgtgatgga gctgagcgaa
     4081 caccttttcc ggcatgaagc ggggcggatg gtggccacgc tgactcggat cttcggcgtt
     4141 cacaatctgg gcctggcaga agacgtggtg caggaggcct tctgccgggc gctggagatc
     4201 tggaagttcc ggggcgttcc ggagaatcct tcggcgtggc tgatgctgac ggcgaagcac
     4261 tgcgcgctcg atgcattgcg gcgcgagcga acggcgagga cgtttgcgcc ggagctgacg
     4321 cggcagctgg agagcgagtg gacgctggcg ccgacggtgg aagaggcgtt tgggccggcg
     4381 gcgatcgagg acgatgagct gcgcatgatg ttctcgtgct gcagtcctca tctggcggag
     4441 gaggcgcagg ttcctctgat cctgaacatc ctgtgcggct tcagcgtgga tgagattgcg
     4501 agcgcgttcg tgagcagcca cgcggcgatc gagaagagga tcacgcgttc gaagaaggtg
     4561 ctggcgcggt cgcggaaatt gtttgacgtt tcgggagcgg cggacttcgc gcggcggctg
     4621 cccgcggtgc agcgggcttt gtatctgctg ttcaacgagg ggtatcacgg ggcttcgccg
     4681 gagtcggcgg tgcgggcgga tttgtgcaga gagagcatgc gcctggcggc gatgctgctg
     4741 aagaatccgc tgggggcgag gccgtcgacg tatgcactgg cggcgctaat gcatttgaac
     4801 gcggctcgac tcccggcgcg actgaatggg tcgggacatc tgacggcgct ggcggatcag
     4861 gatcggtcgc tgttcgatcg ggagctgacg cgggaggggc aacggctgct ggagcagtcg
     4921 gcggcggggg atgaggtgtc ggactatcac ttcgaggcgg caattgcgtg gctgcacacg
     4981 tcggcggagc gcacggagga cacggactgg ggggcgatca ttggtttcta cgaccagttg
     5041 atggcggtgc ggccgtcgcc ggtggtggcg ctgaaccggg cgatcgcgat tgggcagcaa
     5101 gagggaccgg agcgggggct ggaggcgatt cgtgcaattg aggacgcggg gcgtttggcg
     5161 cggtatccgt tctaccatgc ggcgatgggg gaactggagc tgcggagccg gaagaaggag
     5221 gcggcgcggg ggcattttgc ggtggcgctg gagttggcgc ggaatgcgat ggagcgggat
     5281 ttttttgagg ggcggatggg ggagtgcggg tagtgcagga tgtgatccgt tacacagggt
     5341 cgcgcgtggc gcgactccca catccccaac tgcagggatg tggggcaccc ggcgatgagc
     5401 cgcttacttt ggcgagaggc cggcgttggg gtgcgctggg gattcgttgc cggggccgtt
     5461 ggtgacttcg agggagggga tgcgatcatc catcgatggg agcggcggga agggctggtg
     5521 gggttcggtc tgataccagt aggcgacgga gtagaaattg tcggagcggg cgttggcgtg
     5581 gccgtgctcg atggtggcct tcatggattt cgtaaacgtg atgggcgagt cgaggtggaa
     5641 gcgatagacc gccgaccgtt cgccggcgag ttccctgccg accatgggcg cgccgtagag
     5701 gggataggcg aaggcggcgc cgccaaagtc ccaggcaccg aggaagtagt cctctgagcc
     5761 cgtgccggtg accggggtgg cttgcccatc gatgaagaac atgggatcgc cttcacccca
     5821 ccagccgtcc tgattctgca gaacggacat ggtgacgccg atgtactggc cgcggccttt
     5881 ggcatcgaac cagacgtagt tgtctttgcc atcgggattg cgtttgtagt tgatgagggg
     5941 atcgccgttt tcgtaccact ggctggtcca gccgtgattg ggctgggcct ggcggtactc
     6001 ggcgtggaag tagagagtgg aggacgggag tggatgcgtg taggtgcggt agtcgatgtt
     6061 gtagtagagg ttggagagct ccatcttgcc ctcgttggta atggtgatgc gggcgtgatg
     6121 ctggaagggc atggggaaga aacagttcat agcgcgtgcg ctgccgacct gaagcatggc
     6181 ggactgccag ttgaagtatt cgccgaggcc gaggccgaag aagtcgccga tgggggtttc
     6241 gacgctgggg gactcttcgc catcccagta gatgcggaga acgatgcgtt tgaggtggta
     6301 gggctcgggg ctggagatgg tgaaccagag gtgagagatg acggcggggc cgtcgacgtc
     6361 gaggatggtg agggtttggc cgggagtgat ggtgcgggag tcgtggttgg cgccggtggt
     6421 ttcgaggctg gaggcgaggt gccaggtgta gtcctgggcc tgggtgatat cgggccactc
     6481 ggtggggctc tgggcgacgg cctggagtga ggtagcggcg gcgacgaacg cgagggcggc
     6541 catccagcgc atggttttct cccgtgatgt tttttgggcg ggcggagttt tttgaaggcc
     6601 cgcgaacaac gcctgccagt gtagcgcggt ggggcggtga tacgtttttt tgaggtcgtg
     6661 atgcgcaggg agaggaacga atatccggcc tggggtcagg tgcgtccatg tttcgcgatc
     6721 aaaaaacgca ggtccattcg actcgcgtct gaattcattg ttgcgcgcga gaaacgcgcg
     6781 caacgaacgc ggatgcttcg ctcaggatga caggggttct gaacggtgga gggggcgatg
     6841 tgcggacggt attatcgacg gtcggataag cagcggattg cggaggcctt caaggtgggg
     6901 gtaccgcctt catttgagat tctgcccgat tacaacgtgg cgccgacgac gtttcagccg
     6961 gtggtggtgg aggatcggga gacgggcgag cgtgagattc gggcgatgta ctgggggttg
     7021 attccgtact gggcggaaga gccgaagaag ctgggggtga gcacgatcaa cgcgaaggcg
     7081 gagagcctgc tggagaagcc gatgtggcgg gagccgttcg agaggcggcg gtgcctggta
     7141 ccggcggacg ggttctacga gtggcagaag ttcagcgaga agacgaagca gccatgggcg
     7201 attgggttga aggacgatgg gctgctggcg tttgcgggat tgtgggatcg gtggcgttcg
     7261 aaggacaagc agaccgtgct ggagagtttt gcgatcgtga ccacggaggc gaatgagctg
     7321 actgccccaa tgcacgaccg gatgccggtg atattgaagc ccgcggatta tgagcggtgg
     7381 ctgcggcggg aggatgcggg gcggccgccg ctggatttgc tgcggccgtt cgacgcggat
     7441 cggatgcgga cgtggcgagt ggatgcagcg gtggggaatg tgaagaacaa ttctgcggcg
     7501 ctgctggatg aggttccggc ggggacgctg ttcgggtgaa tagcagcgtt cggcgttcgg
     7561 cgttcagcgt tcagttggct cgaggccggc ggtgatcatg ggcaggcctg ttgcgggcca
     7621 gtgactgacc tgagcgtcgg tgttgagggc gagggcggag tcggcgttgg agacgggggt
     7681 gacgtcgatg ttccagccat cgacgacgac caggggcgtg tctcctttga gatcggcgag
     7741 cgcagcgtcg atggtgacgg tgcgggtttc gccgggtgcg agcgagatgt agttgtcgga
     7801 gtagaagacg gggaggacgc ggtcgctggt ggacttgcgg cggagttgca ggtgcgccat
     7861 gagggcgatg gaggggccgg ggttcttcag cgtgacggtg aggccgagac gggcggcgga
     7921 atcggatccg gaatcgtggc gggcgacggt ggcttcgagt ttaacggtgg ggagctggtt
     7981 gaggggcgtg aggtcgtcgc cctgcgaggg gttgggctcc cagtagaggt tgcgggagag
     8041 gatctggccc tgggcgtcgc gcagttcgag ctcggcgaaa tagagcgagg agagatgctc
     8101 agggagcgga atggggccga ggtcgatggc ggtgtcggcg ggaccgctga cggggatgtc
     8161 tttttcgagt gcgggctggc ctttgcccca gagcgtatag atggcgatgt gcgcgatgtc
     8221 gccggagaga tcggtgggga ggttattgat gacgcggagg tggaagtcgt cctcgtccat
     8281 ctggatgtgg acaagttcgg aggcggactt cacagcaaag agcgaagcgt tgggctcgag
     8341 gtcgtagtgg tagagctgcc agacgaagct gggctgcgcg ggattgctca tccaggtgat
     8401 gacgccggtg gcgggtttga agaggagctt gttgcggccc tcgtacatgg cgcggtaggc
     8461 ctcgtagttc atcatctggg ccttgcgcac gaagtcggct aagttggcga tgcggccgta
     8521 gcggccggcg aggacatagg ggtaggtatc gccgtgctgc gcgcctttgg cgaagtcgtg
     8581 cgcggcccag tcgtcgttga tggtgttcca gtccttctcg ggcatcatgc cgtggatgga
     8641 ttcgagggtg gggacggaca tgctgccagt ttcggttttg aacggcgcgt cgaagacgta
     8701 gaacgcgcgc ggctcacgcc aatagtaagg accgctggac atgacaccgc gtccggcagt
     8761 ggagcttggc tgataaaggc gggcgggatc gagttcgggg atgagcttgc gaagggcggc
     8821 atcgatttcg ggcggcggat agccttcgtt gcgcgcgcac cagagagcga tggaggggtg
     8881 attgcggtag cggaggattt tgtcgcggac gttggccatg tagatggcga agtcgtcggg
     8941 attggggccg tcgctgggat tgggctggaa gaattcgtcc cagatgagga tgccgtattt
     9001 gtcgcagagc tcgtagaagt cgtcgccggt ggactgaccg acccagttgc ggatcaggtt
     9061 catgttggcg agggcgtgca tgtgaatctc ggcgtcgagg cgggcgcggg ggatgcgctt
     9121 gagggcttca tcgagtcccc agtcgccgcc gcggatgaag actttgacgc cgttgacgga
     9181 gatggtgagg gcgtccatgc cgggggcttc ataggtgatc ttgcgaatac cgaaggtggt
     9241 cgagtgcgcg tcggagtcgt ggccgtgttc gacgaagtgg agatggagcg tgtagaggtt
     9301 ttgcggcccg tagccgttgg gccaccagag ttttggattc gcaagatgga gcggggcgta
     9361 ggttttggga tcgaaggtga cggtctgggt ttcgttgggt ttgagggtga ctttctgcga
     9421 gaaggtgatg tcaccaatgg tgccttccag ggtgcctgtc tgcggtttgg gcgaggtgtt
     9481 ctggagcgtg gcgttgacgg tgatgtcggc ggagtcggtt ttggggagcg ggaggtcggt
     9541 gatgacctgg ggatcgttga tgagaacagg accggaggca gagagccaca ctttctgcca
     9601 gaggccggtg tcgcgatcgc ggacagcgtt gagccagtcc caaccgatgg tgcagaggaa
     9661 ggtggggccg tcgatggcgg tgatgccgcc gtttttaccg acgccgttgg cgatggtgtg
     9721 ttcgtgggga tcgccgggat gcggttgagg ggagactttg acggcgagaa cggcggttga
     9781 gccgggcctc acgagcgggg tgatgtcgaa gcgtccacgg atgaaggcgc cgttgatggt
     9841 gccggcgtgg gtgccgttga cccagacttc ggaagtgaag ttagcgccgt cgaagtgaag
     9901 ccaaacctgg tggtgggcgt agttcttcgg tatcgcgatg gtagtgcggt accagtacga
     9961 ggttttgttg agggactcgg ggatgatggt ctcgcggttg ttttcgccgt aggtcggttc
    10021 ggggtaaacc ttgtcgttga caagactggt gaggacggtg ccggggacgg tggcggcgag
    10081 ccatccggcg ggctgatagc tcgcggagga aacggtggcg ccggggtcgg agaccttggc
    10141 ggagtcctgg agctgccagt tggaattgag ttcgatggga tccgggacgg cgttcctttg
    10201 ggcgaaggcg gcggcggctg tgagggcgag gcagatgacg gcgaagggaa cgaagagtct
    10261 gctggaagga gccacgggtt tcctcgtttg gttgggtcgg gggcgtcggg aatcggccct
    10321 gtccagtatt cacaaagttg gagcgggtag ccagcaggga aaggtggagc gttgcaggtt
    10381 cggcctgccg ggttccgaaa tagaaccggc tcggtacttt cgggtggacg gtgagcggat
    10441 tcggagttga aggatgagcg gcgaaggagt cttctgcttg ctgtggggtg aacctggcga
    10501 tcgggtccgc tgcggggggc ggcgatgttc tttcctgttt tgggtatgag gttccgattt
    10561 gggtcgtgag cggtactttg ggctggcatg ccagccgagt ttccttgcct ggagccgtct
    10621 ccgaggagtc ttctgcttgc tgtggggtga acctggcgat cggccagagg gggcgcgctt
    10681 gaagacgccg cctgtgtttg gggttcagtc tgcttcagtc tgcttcaatc tgcttccgtc
    10741 tgcggatttg tcggaataga acggacgctg tacttccggt tcgatggtga cgaccccgtg
    10801 ttgaggactg agcggcgaag gagtcttccg cttgctgtgg ggtgaacctg gcgcttttgc
    10861 caattccggg agccgatgtt cgggttggga cgggccagcc gctcggccgg gggcctgcgg
    10921 taagcggcga gatgcttcgc cgacgaggac gtcacggcac ggatatcttc cgacaggttg
    10981 ggaaattcgg gtgtcgtaaa ccgtgtgcag cggctatcct tagcgtgcct gtgaggaagc
    11041 gcggcggatg agaattgcca tcgtcacgga gacgttcctg ccgaagattg acgggatcgt
    11101 caccatggtg acgaagacgg tggagacgct gagggcggcc ggcgatgagg tgatggtctt
    11161 tgcgccgagc ggcggaccgg cggagttgtt cggcgccgag gtcgtgagcc tgccttcgat
    11221 tccctttcct ttttatccgg agttgaatgt ggcggctccg cgggcgtcga tgcggaagca
    11281 tctggagcgg tttcggccgg atgcttttca tctcttcgag ccgtcgctgc tggggatcgg
    11341 cgggatctat tacggcaagg tgctgcacat cccgattgtg atttcgtatc acacgaatct
    11401 gccggcgtat ctgcactact acaggctggg gttcttcgaa gggccaacgt ggaagctgat
    11461 gcgggcgcgg catctgcggg cggatctgaa tctgtgcacg tcgacgcaga tgatggagga
    11521 gctgcatcac catgacatcg accggctcgc gctatgggaa cgggcggtcg atgcgcagcg
    11581 attccatcct tcgaagcgct cggcggagat gaggagtctt ttgagcggcg gcgagccgca
    11641 gaagccactg atcctgtatg tgggaaggct ttcggcggag aaagatgttg aaacgctgcg
    11701 cacgctcgtg gaggcgatgc cggagacgcg gctggccatc atcggcgatg gaccggtacg
    11761 gaaggaactg gagaaacact ttgaaggaac gccgacctac tttgcgggtt acatgcgggg
    11821 agaggcactg gcgcaggcgt atgcatcggc ggacgtgttt gtgatgccgt cgaagacgga
    11881 gacgctgggg ctggtgttga tggaggcgat ggcggcgggg tgtccggtgg tggcttgtcg
    11941 agcgggcgga gtaccagacg cggtcgaaga tggagtgacg ggattcctgt atgagccgga
    12001 tgatccggat ggtttggtga agaaggtggc gtgggtgctg gaacataccg ccgagcgaac
    12061 cgaggtacag gtgcaggcgc gcgccgatgt ggagcgacac agctgggagg gatcgacgga
    12121 gcagctgcgg cggtattatg cgcaggcgat cgagcatccg cgaccgaagg gagtggctaa
    12181 aggccagatg cggtggagag gggtgacgcg gcgagtggtc gtggcttcgc tgaggaggct
    12241 acttccctga gggcagcggc gcagggttgg gctggaggct ggcctgggcg gcagccggag
    12301 cgggggaatc cattttgtag gcgccgtagt cgatagtgag gacggcgtgc ccgccgaagc
    12361 ggacgtcggt atcgctctcg ttgtgctcgg gcagccagaa ctctccggtt ctggagttga
    12421 cgtgatgaat cttcgtgcca cgaatccaga aggaaggatt gtgtgcgggt tcggcttcga
    12481 ttttggcaac ggcgaagtcg tcggcgtcga cccagatggt gccgcggtag aggagtttcg
    12541 agttggtatg gggctcgacg cggaggagat agcagggacg gccgtcgagt agctgctggc
    12601 cgacgagggt gaagttgtag ttggcggtgg tgggggcggt ctggcggggg tcggcggtcg
    12661 attcctgttc ggctgcaaag agcttcttca ggacacggtc ggcgagcatt ttcgatccgg
    12721 tctgggagac gatgcggaac tgcttggtgg caggagctga ataggtgacg gcgacgacga
    12781 tggtggagct gagattagcg gggaacccgt ggtaggagac cgagtaggtg cggagctcgg
    12841 agtagccttt gaggtcctgg gcgcgtttct tttcgccgtt agcgaggcgc tggacgatgt
    12901 cgtcgagggc gaggttgccg gcggggcggg aggccacggg agggtctgac agatttgtaa
    12961 tcgccgcgtt aacattttgg gcggtgagga aggcgggcga tgcgaaagtt gcggcggtaa
    13021 ccagggttgc cgcgagagtg gccgtgcgaa cggttggacg caagatgccc tatgatgccg
    13081 cctgggagag agactcgtca atccaacagt tgcatcattc ttcattccca aatgtgccgg
    13141 cgtactgtag cgtagaacga aatcggcacg actaacggat ttctgtttcc tgtcccctgt
    13201 agtcattctt ttggttcccg ggttcggctt gctgtcccga ggaaggcgct cctgacagac
    13261 acgatgaatc tggagcgttc ccgaggctat gtcggggacg acggaggcgg agtgcacaac
    13321 gagttgttgg tgctgtcccg agaccaggac aggttgtccg catggcaaag cggagtttcc
    13381 cttcacagcc atacgcggca ctcgcaggag agtctgggtt ttcttgcggc gtttttcgaa
    13441 cggcgtgcgc tgacacggcg atggtatgca tgccagcggg aaagttgccg gcgtgtaacc
    13501 ggaatcgacc tgaatcttga gcgggcacac tggacgccgc cgctgtgcga gcggatggcg
    13561 caggatgtgg agcaacggca aatcgaacaa ctgggactgc ggccgatggt gtcgctgacg
    13621 gaccacaaca gcattgaggc gtgcatgctg ctgcggcagg atgcggcgtt tgcggatgcg
    13681 ccgatttcga cggagtggac ggtaccgttc ggcggggcgg tatttcactt cggaatccac
    13741 aatctgccgg cgcgatcggc gcaagatctg atgggggcga tgcacgaggc gacctatctg
    13801 gccgatgagg atcgcatcct gaggttgttt gcggagttgt cggcgatcga gggtgtgctg
    13861 gtggtgttca accatccgtt gtggaacttc tacagcattc cggcggaccg gttcatgtat
    13921 gaactgacgc gattcctggg gatggggaat cggtatgtgc atgcatttga gctgaacggg
    13981 atgcggagtc acgcggagaa tcgcgcggcg ctgaagcttg ccgcggagtg gaaccaggtg
    14041 gtgatctcgg gcggggaccg gcatgggcgc gagccgaatg cttcgctgaa tctgacgaat
    14101 gcggcggact ttccggagtt tgtggaggag gttcgcgacg gacggcagag catggtgctg
    14161 gtgatgcccc agtatgccgc gcccctgggt tggcgtttat accgaaactt cacgcacgta
    14221 atcggggagt atccggagca tccggagggg cgccggcgat gggatgagcg gacctatcat
    14281 ccggatcgcc atggagctgt ggtcccgatg cggttgctgt ggaagagcgg cgaaccagct
    14341 gagtttctga aggcgatgtt cggtgcggcg atgatggctg gacgagtacc ggtcgatggt
    14401 ctgtggcgaa ggttgatgcg gcgggagaat gagtcgctgc agttgccgca cagggtcgag
    14461 ggtgcagggt acagagtgca ggtcacagga cacagatcgc aggcggtgga gttagccagc
    14521 gacgggggcg agttgtagct ctttttccag aagtgcgaag tggtcgcggt cggtttcgca
    14581 ggcgccggtg tgggggagtt gctggaccag agcgttgccc ttttcccagg cttcgcgggc
    14641 ttcgccgatg cggtcttcac tccagaggaa ggcgctgcgt gcaatccaat agtcggcatt
    14701 gtggcgggtg gggtgtttgg cctgcatgcg ctcccaccag aggcgtgtgc cctcggggtc
    14761 gtgttcgagg caggcttttc cgaagatgaa gggcgtgtgc agctcagcgg ggatgtcgga
    14821 ggctgattcc tggtagatgg tctcggcttc ggcgagggcg aggctggctt cggagacgcg
    14881 gccgcaatcg agatagtagg aggcagcgag caggcggagg tggagtgcct gctggccgat
    14941 ggagatggat gaaccggcgc gcaggatggc gtcgatgtcg tagtcgcggg ggcgaagagg
    15001 ggagaccgta gtggcggctg cggcggagaa ggcgcgatgg aggtcggccc aggggccgcg
    15061 agagagaagc tgcaggatgc gggcgccgtc agagtagaag gctttggtgc ggaagggaat
    15121 gaggttgagg acggcggaga gcagagagag ggtggcgaag aaggcgagcg ggagccattc
    15181 ggcctgccag ggctgaccgg gagccccgag ggtgagagcc acagcggcga gaccgccgat
    15241 gaggctgaag aaggggccgg aagccaccat gacgatgtcg cgccagagag ggcggtgggg
    15301 gtccttgggg gcgacggcgg tggctccgcc gatggcgagc agacctgcaa ggacgaagcg
    15361 aaattcccag tggccgtcgc gcatgcgacc ctggaagggg ccgacgatga aggcgcggag
    15421 cttcatgccg agggcggagg ccgcggcggc gtggccgaac tcgtgaaggg cgaccataag
    15481 gacctcgagc aggagaagca ggaagaaatc gtgaaggaag gagctgcgga tccagacgag
    15541 gccattggat tccgcccagc gccaccacca ggtcatggcg gagatgaatc cgccggtacc
    15601 ggcgacccag atgaggcggt tgacgagggc gtgggttcca tcgccgggga gcggctgcat
    15661 gagctgctca gcctggacgg catcacggcg gcggaacgcg atgaggccgg ctagtccgac
    15721 ggcgaggaga agtgcatcgg ggttgaggaa ggcggtcgcc ggggcggtga ggaagcggcg
    15781 atccatgtag aggaagaggc agccgagaag ggtgttggcg gcgctggcga gaaggcccca
    15841 ggcccgtgcg gagggcttac cgacccagaa ggtccagcag gcggcggcgt agatgacgcc
    15901 gaggagcggg gtggcggcgt gaaccgaggc tttgaggaag gtggggggaa ggtgcgcttc
    15961 aggccaggag gcgatgtcga ggatcgcctg aagggaggag cgcaggaaca ggatcgcccc
    16021 caaggcgaac atccagcaca gaaaatttcg taccgcgcgc ataaccttcg cctggaacgg
    16081 gagttagcgg cagagtaatg gggctgggga ggggcaaaca atgctacttc ggagggaggg
    16141 gatgggcgtt cggcgcaccg cgttccgcgt tcggctccga catccgattc agcaggagga
    16201 ggtggcgagg ggtgcaatga gcgccggggc tgggacgccg tcgagagcgg gggttttgag
    16261 gggaacgcag caggatttgt tgaggcggtt gagatccgcc tgcatggcct tgtcctgccg
    16321 aagaacgatc agggtttcgc gcaggatttt ggcggctccg gagctgggag ggacaacgat
    16381 ccgatagtgc atccatttcc cttcgcgccg cgcggcgacg acgtcggccc gcctgaggta
    16441 ggcaaggtgg cgggagattt tgggctgact ctggccgagg atttcgacga agtagcagac
    16501 gcagatctcg cggccgtcca ttaggttgag gagcctgagg cgggtgcggt cggcgagggc
    16561 ggcaaagagg agcgcgaggt catagctctt tggggagcgg ttcacgcata catgatatac
    16621 gccttgacgg atatgtccag cggagaatac attcgtttta gcgtatgtaa ggaggcttct
    16681 catgggatcg acgaccagcg ttcaggaaca ggttaaggcg aagtatggaa gcgccgcacg
    16741 cgcggtggca gaagcgggca cggtgcaggc gtgttgcgat ccggggctgc gttgctgcga
    16801 tccgatcacg gcgaatctgt acggcgcgga gcagaagggg atggttcctg agaaggcggt
    16861 gcgggcatcg ctgggttgcg gcaatccgac ggctctggcc gaactgcggg cgggagagac
    16921 ggttctcgat ctgggttcgg gcggcggcat tgatgtgttg ttgtcggcgc agcgggtggg
    16981 accgagtggc aaggcgtatg gcctcgacat gacggacgag atgctggcgc tggcgcgagc
    17041 caatcaggaa caggcgggcg tgaccaacgt cgagttcctg aagggcgaga tcgagaacat
    17101 cccgctgccg gacgattcgg tggacgtaat catttcgaac tgcgtgatca acctttccgc
    17161 ggataaggat cgcgtgctgc gcgaagcgtt ccgggtgctg aagccgggcg ggcggtttgc
    17221 cgtgtcggat gtggtggtgc gcggcagcgt tccggacgaa gtgcggagaa gcatgttgct
    17281 gtgggtgggc tgcatcgcgg gagcgctgga ggagaaggaa tacggggaaa agttggcggc
    17341 ggcggggttt gtgaaaacgt cgatggagac gacacgcgag tacaacgtgg aggatgcacg
    17401 ccagttcctg acggaggcgg gtgtagatgt cgatgccatt gcgccgcagg tgaacgggaa
    17461 gttcttcagc gcgtttatcc gcgcgaacaa gccgcagact gcatgctgcc agcccggctg
    17521 ctgcacgtcg gcgggagcga gggaataacc acaatggctg gtccttacca cgttctgttt
    17581 ctttgcacgg gcaactcggc gcggtcgatc atggccgagg cgattctgaa ccacaaaggc
    17641 aagggcaagt tcacggcgta cagcgcgggc agccatccga cgggacggcc tcgaccggag
    17701 gcgctcaagc aaatcgagtc agcggggctt tcgacggcag ggctgcgcag caagtcctgg
    17761 gatgagttct cgggtggggg agcgccgaaa ctggattttg ttttcacggt gtgcgacaac
    17821 gcggcgaagg aaacttgtcc ttactggccg gggcaaccga tgacggcgca ctggggagta
    17881 gccgatccgg cggcggtgaa ggggacgccg gaagaaattg ggcgggcatt ccgggaggca
    17941 ttcacggtcc ttgaccggag gatcgggctg tttttggcat tgccgctggc gacgcttgag
    18001 aacatggcaa ttcagcggga gatcgacgag atcggtcaac ggtagacgtc gcgccagagt
    18061 atccgctgct aaaaaccagt tgttttcacg ggatcacggt tcggttcgat gctccaaatg
    18121 cgaatctgag ccagtgactg agacggcact tacgccgcga gggctgggtg tgattcactc
    18181 cggggcatcc tttccccccg cattcccacg aaaattcacc cacggagaaa acatgagaat
    18241 gagattgtgg agtgccctgg cgggcctaag cagcctggcc gggttcgttg tatgtggagt
    18301 gtcatcgcac gcgcagtcgt cgtctgcgca ggcgaaggcc ccgatgtaca gctatgttgc
    18361 gaactggcaa attccgcgct cgcactggtc cgaagtggcg cagggggccg cggcctacaa
    18421 aacagtggcg gataaggcac tggcagatgg cacgatcgtg ggctacggct tcgatgaggc
    18481 agtcgtgcac accgccgatg gcgatacgca cgacgactgg tggtcgtcga tgtcgatcgc
    18541 cggactgctg aagatgcgga cacagctttt tgcggccggg acccctactt cgcctgcaat
    18601 ggatgcggcc acaaaacact gggacgagat cctgatgagc cgttattaca acaagaagtc
    18661 gggttcctat aagaacgcgt acacctgggt ggcgatgtat cagctgaaac cggacgccgg
    18721 ggatgatgca gtggagatgc tgagccggaa cgtcattgcg ccgttcctgg agaagctgtt
    18781 tgccgacggg acgattctgg aatacgaaat cgacgagcag gcggtgcact cgcaggcgcc
    18841 gggcacgttc tctgtggtgt gggtttcgcc gacggcggag ggcgtggaca aggtgaatgc
    18901 ggctctgttt gaaacggtga aagctcagcc gatgatcgag ccggcatttg gttcagccac
    18961 aaagaacagc gcgcatcggg atgagctctt gttgagcgag ggagcgtaca agtaggggtt
    19021 ttcccactcg ggcgcgcgat gatgtcgcgc gccgg
//
LOCUS       DASZYN010000169         2281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_842560, whole genome shotgun sequence.
ACCESSION   DASZYN010000169 DASZYN010000000
VERSION     DASZYN010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842560"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VHE33_04320"
     CDS             <1..334
                     /locus_tag="VHE33_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96707.1"
                     /translation="VIHGFIGLPIFTWWLAIVWGLLVGFIGFLMVSTWRFWSGKEINL
                     TVRQPFRWIVLLGVSIYLLVFFSRAVLLLLALAYMFSGVIARLAYSWRRHHPHARNPL
                     PSSQGPMG"
     gene            331..1386
                     /locus_tag="VHE33_04325"
     CDS             331..1386
                     /locus_tag="VHE33_04325"
                     /inference="COORDINATES: protein motif:HMM:NF014793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HVT96708.1"
                     /translation="MTDPQKIAIVGAGSLRGKELNDSLADSPFAGAEFLLMDDESQLG
                     NLEAVGDEVSFIQRIDPDSFRRVDFAFFTGDQDLTLKHWESAHKAGAGIVDLSYALEG
                     KPGVLVRAPWIDQALQPAPAQPGSRTPGLETPAIVPAHSVAVALALLLGRIAQSAPIR
                     IASCTVLEPASEYGRAAMDELHQQTVKLLSFQELPRQVYDTQVAFNTTPAFGDSAKFN
                     LATSESRIRRHFNLLAAGRLGDVSVQLLHTPVFHGYGISLAIELTTPTSLDQMEASLA
                     GEHVEIVTAESDPPTNLTVAGQANILVRVRPHNTRDPETTRFWIWAGFDNLRFAVLNA
                     LACALELKRLRPSGEVQ"
     gene            complement(1767..>2281)
                     /locus_tag="VHE33_04330"
     CDS             complement(1767..>2281)
                     /locus_tag="VHE33_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HVT96709.1"
                     /translation="GHTDIGFISGPMMLRSARVRREAFIGCLERRGIGADWIEEGNHQ
                     VDGGHDAMARLLGRKTRPTAVLASNDMTAIGALGAIHERGLRVPEDISIVGFDDIQIS
                     AFTQPALTTVRLSREEIARVAFRALYSIRQDAGKGAEYGVQPALVVRRSTGRAPRGAG
                     AAEREPVLVS"
BASE COUNT          408 a          897 c          561 g          415 t
ORIGIN      
        1 cgtcattcat ggcttcatcg gcctgcccat cttcacctgg tggctcgcta ttgtctgggg
       61 tctgctcgtc ggcttcatcg gcttcctcat ggtcagcacc tggcgcttct ggagcggcaa
      121 agagatcaat ctcaccgtcc gccagccctt ccgctggatc gtgctcctcg gcgtgagcat
      181 ctatctgttg gtcttcttct cgcgcgcggt gttgctcctt ctcgctctcg cgtacatgtt
      241 ttcgggcgtc attgcccgcc tcgcttactc ctggcgtcgc catcacccgc acgccagaaa
      301 tcctctgcct tcttcacaag gtccgatggg atgaccgatc cgcaaaaaat tgccattgtc
      361 ggtgccggct cgctgcgcgg caaagaactc aacgattctc tcgccgactc acccttcgca
      421 ggcgccgaat tcctgctcat ggatgacgaa tcgcagctcg gcaacctcga agccgtcggc
      481 gacgaagtct ccttcatcca gcgcattgat ccggactcgt tccggcgcgt cgacttcgct
      541 ttcttcaccg gagaccagga cctcaccctg aaacactggg agagcgcgca caaggccggt
      601 gccggcatcg tcgacctctc ttatgctctg gaaggcaagc ccggcgtcct ggtccgtgcc
      661 ccatggatcg accaggccct ccagcccgct cccgcgcagc ccggctcgag gacccccggc
      721 cttgagaccc cggccatcgt accggcgcat tccgtggcgg tcgctctcgc cctcctcctc
      781 ggacgcatcg cgcagtccgc gcccattcgc atcgcctcct gcaccgtgct cgagcccgcc
      841 tccgaatacg gccgcgccgc catggacgag ctccaccagc aaaccgtcaa gctgctcagc
      901 ttccaggaat tgccccgcca ggtctacgac acccaggtcg cctttaacac cacgcccgcg
      961 tttggcgatt ctgcgaaatt caacctcgcc accagcgaat cgcgcatccg ccgccacttc
     1021 aatctgctcg ccgccggccg cctgggagat gtcagcgtgc agctcctgca cacgcccgtc
     1081 tttcatggat acggcatctc cctcgccatc gagctcacca cgcccacttc cctcgatcag
     1141 atggaagctt cgctcgccgg cgaacacgtc gaaattgtca ccgccgaatc cgatccgccg
     1201 accaacctca ccgtcgccgg ccaggcgaat atcctcgtcc gcgtccgccc ccacaacacc
     1261 cgcgaccccg aaaccacccg cttctggatc tgggccggtt tcgacaatct ccgcttcgcc
     1321 gttctgaacg ccctcgcctg cgccctcgaa ctcaagcgcc tgcgcccctc gggagaggtg
     1381 cagtaaacga atttgcggag aaaaatgccc agcgcagaag tgcgaaggga acgagcagca
     1441 aagaggtatc cgtaaccgcg ccccgcagcg aaccaggttc agtagaaagc ctcctcccgg
     1501 cctggctccg ccacaacccg tcccaaccgc gtgggtgcaa cccggcagca aagggctatg
     1561 ctcccgtccg caccccaaaa aacaccattt ttggccaggt tcaccccaca gcaagaagcg
     1621 gactcctttt gcgcgcaaga atcaatgcca acccgccctt gctgcgcacc aaagtaccgt
     1681 ccccgcacca cccaaaacca aattgggaaa gtgtaacctg ttcatctcgc cgggccgcag
     1741 cacgctccga atcaggcgac tgcccctcag ctcaccagaa ccggctcccg ctccgctgct
     1801 cccgcacctc gcggcgcacg ccccgtcgac cgccgcacca ccagcgccgg ttgcacgcca
     1861 tattcggcgc ccttgcccgc atcctggcga atgctgtaca gcgcccgaaa cgctacccgc
     1921 gcgatctcct ctcgcgacaa tcgcaccgtc gtcagcgccg gctgcgtgaa ggcgctgatc
     1981 tgaatatcgt cgaaccccac aatcgaaata tcttccggca cccgcaatcc ccgctcgtgg
     2041 atcgccccca gcgccccgat cgccgtcata tcgttcgacg ccagaaccgc cgtcggccgc
     2101 gtcttccgcc ccagcagccg cgccatcgca tcatgcccgc catccacctg gtgattgcct
     2161 tcttcgatcc agtccgcccc gatcccccgc cgctccaggc atccgatgaa cgcctcccgc
     2221 cgcacccgcg ccgatcgcag catcatcggc cccgagatga atccgatatc cgtgtgtccc
     2281 a
//
LOCUS       DASZYN010000170         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1099736, whole genome shotgun sequence.
ACCESSION   DASZYN010000170 DASZYN010000000
VERSION     DASZYN010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099736"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VHE33_04335"
     CDS             <1..535
                     /locus_tag="VHE33_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96710.1"
                     /translation="LPSHRVIGSGTVLDSSRFRFLLAQRCAVAVQNVHAYIAGEHGDT
                     EIPLWSSASIGNVPLLDWNRPAPLTPAERSSIFHSVRTAADRVIAGKGATNYAVGLAV
                     SGILQAILGDEGRVLPVSSVLSEYRGLSDVCLSMPAIVNRRGVDCLLPVPLSNEEHTG
                     LEASAAAIRNVVRSLEF"
     gene            complement(548..1678)
                     /locus_tag="VHE33_04340"
     CDS             complement(548..1678)
                     /locus_tag="VHE33_04340"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HVT96711.1"
                     /translation="MRPSFTLGIEEEYQTVDPVTRDLRSHIATEMLAQGKVRLEERVK
                     AEMHTSVVEVGTRICRTIEDARQDLYQLRREMIKLAHEHGLVLVAGATHPFADWRTQE
                     IYPDPRYHQVVKDLQLVARSNLIFGLHVHVGVEDREAAIRIMNSMRYFLPHIMALATN
                     SPFWLGLNTGYKGYRSKVFENFPRTGIPDAFSSYSEFENYVALLVKTNCIDNAKKIWW
                     DIRPHPFFNTVEVRACDIPLRAEETVAIAALIQATAAYLYRLHAGNQDYRQYARALLM
                     ENKFRAVRYGLDGKLIDFGRQEEVPVRELMEEYLALIEPEVEELGSRDAIEHIRTILE
                     TGTGADRQLKVFEETNDLKAVVDYMAEETKAGLFEPAAQSVA"
     gene            complement(1779..>2444)
                     /locus_tag="VHE33_04345"
     CDS             complement(1779..>2444)
                     /locus_tag="VHE33_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96712.1"
                     /translation="FLKPVNGGGWRDVHHVHSREEFFQAYDQSRDLCMMLQRGVKFTE
                     YFRCYVVGQEKVRIMYYDPRRPHAERYVKNPKPADKKLLDKVETDARKLCQALGYDLN
                     TVEFAVEDGVPYAIDFMNPAPDADLHSVGKENFDWIVEAVADLAIAKARIARPHVPEF
                     HWSAYLGAASDTAKAAAKKAASPAKKAAAAKPAAKKTATKKTGKSTGAAVGQPKATVS
                     AIE"
BASE COUNT          421 a          772 c          718 g          533 t
ORIGIN      
        1 gctgccctcc caccgtgtga tcggcagcgg cacggtgctt gacagctccc gctttcgctt
       61 cctgcttgcg cagcggtgcg cggtcgcggt gcagaacgtg cacgcataca tcgccggcga
      121 acatggcgac accgagattc ccctttggtc gagcgccagc atcggcaatg tgcccttgct
      181 cgactggaat cgtcctgctc cgcttacgcc ggccgaacgc tcgtctatct ttcacagcgt
      241 ccgtactgcc gccgaccgtg tcatcgcggg caagggcgcg accaactatg cagtcggtct
      301 cgcggtgagc ggaattctcc aggcgattct tggcgatgaa gggcgggttc tcccagtcag
      361 ctccgtgctg tcggagtatc gaggcctgag cgatgtgtgt ctgtccatgc cggccattgt
      421 gaatcggcgg ggcgtggact gcctgctgcc cgtgccgctc agcaacgagg aacacacggg
      481 tctcgaggcc agcgccgccg ccatccgcaa tgtcgtgcgg tcgctcgaat tctgaattgc
      541 gtgcgaatca ggcgacgctt tgggctgccg gctcgaacag tcctgccttg gtttcctcag
      601 ccatgtaatc caccacggct ttcagatcgt tggtttcctc gaagaccttc agctgccgat
      661 ccgcgcccgt gccggtttcc aaaatcgtgc ggatgtgctc gatagcatcg cgactgccca
      721 gttcctcgac ttctggctcg atcagcgcca ggtactcctc catgagctcg cgcacaggaa
      781 cctcttcctg acgcccgaag tcaatgagct tgccgtccag tccgtagcgc acggcgcgga
      841 atttgttttc catcagcaga gctcgcgcat attgccgata gtcctgattc ccggcatgca
      901 ggcggtagag atacgcggcc gtggcctgga tcagcgctgc gatggcaacg gtttcctcgg
      961 cgcgtagcgg aatgtcgcac gcccgcacct caacggtgtt gaagaacgga tgcggccgga
     1021 tgtcccacca gatcttcttc gcgttgtcga tgcaattggt cttcaccagc agcgcaacgt
     1081 aattctcgaa ttcggaatag ctggagaacg cgtccggaat cccggtgcgg ggaaagtttt
     1141 cgaacacctt cgatcggtag cctttgtatc cggtattcag gcccagccag aagggcgaat
     1201 tggtggccag ggccatgatg tgcggcagaa aataccgcat tgagttcatg atccggatcg
     1261 ccgcttcacg atcctccacg cccacatgga cgtgcagtcc gaagatcagg ttggaacgcg
     1321 ccacgagctg cagatccttg acgacctggt gataacgcgg gtccgggtaa atctcctggg
     1381 tgcgccagtc tgcaaaggga tgggtcgctc ccgccaccag caccagacca tgctcgtgag
     1441 cgagcttgat catctcccgc cgcagctggt agaggtcctg ccgcgcatcc tcaatggtgc
     1501 gacagatgcg ggtacccact tcgacgaccg aggtgtgcat ctcggccttc actcgctctt
     1561 ccaggcgcac cttgccctgc gcgagcatct cggttgcgat gtgggagcgc agatcccgcg
     1621 ttaccggatc gacggtctga tactcctctt cgatgcccag ggtaaaggaa ggccgcatgg
     1681 tgcctgccgt tatatgacgg gaggcagccg cgaatcaagc aaatatcggt cgtgggtacg
     1741 gcatcatcgt ggggagattc agtcccggcg gcagagcgct actcgattgc cgaaaccgtc
     1801 gcctttggct gtcccaccgc ggccccggtg cttttgcccg tttttttcgt tgctgttttc
     1861 tttgccgccg gttttgccgc ggccgccttt ttagcggggg acgcagcttt tttcgccgcg
     1921 gccttcgctg tatccgacgc cgcgcccagg taggcgctcc agtggaactc cggcacgtgc
     1981 ggcctggcga tcctggcctt cgctatggcc agatcggcca ccgcttccac gatccagtcg
     2041 aaattctctt tgccgaccga gtgcaggtcc gcatccggcg ccggattcat gaagtcgatc
     2101 gcatacggca cgccgtcttc cacggcaaac tcgactgtgt tcaggtcata tcccagcgcc
     2161 tggcacagct ttcgcgcatc ggtttcgact ttatcgagga gttttttgtc cgcgggcttc
     2221 ggatttttta cgtaccgctc ggcatgcggc cgccgcggat cgtagtacat gattcgcacc
     2281 ttctcctgcc cgaccacata gcaacggaaa tactcggtga acttcacgcc ccgctgcagc
     2341 atcatgcaca ggtcgcggct ttggtcgtag gcctggaaga actcctcgcg cgaatggacg
     2401 tgatggacat cgcgccagcc gccgccattc accggcttca gaaa
//
LOCUS       DASZYN010000171         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_388998, whole genome shotgun sequence.
ACCESSION   DASZYN010000171 DASZYN010000000
VERSION     DASZYN010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388998"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2107
                     /locus_tag="VHE33_04350"
     CDS             <1..>2107
                     /locus_tag="VHE33_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96713.1"
                     /translation="AFSLAPIPASGSVAGVSVSADALEITVDYALGAAIDARASINGI
                     KVTAGADSVSIPSLTFPPPSGFTITPDLEKAFRLLFSMAIASWVPAQGSGLAALLGLT
                     GGYFGLPADWPLLGPGFLADPLGALRAWLGHIAVDVSADQTPLLPKALEVLPSVFGSS
                     TGVLSGSGIYDDPWAVAIDTGVELLTWFEPNGPPSNWLAPLSGAITAAASFNELFLAA
                     QGLGSFVSGVQEACSTPSATWATGFKSLATWLAGTDGFVPLESQVPATAQWTAGTTLQ
                     SSHMKMPSDAAAITQINAQIASWSPGAKVVLLLGPSFSDHSIWDTFLSAVPLKPNFNF
                     RVPGIDPANVDLLSVTDAADFYTVDLIDNGLAGLAAQIGRVVERIGQLRPGVKVTLVA
                     HSTAGIAARSYAAVNAAAVQGLITLGTPHLGSTLLPITDQPTGSALGVLNTLVPSLPA
                     GSLNDAVAALTLAADGYAPASGAGQLPVPAPFPVAAFADPGSTETGGVPALAIGSSLN
                     ADLLAAVKTGVASLAGGVSNPAPTHLAFGIRSRVDFGTDGDVQVDAFVRADAGRFALS
                     TDAAEPARPAHALNASVTLTRPDDWLAGSPSSPERVRWMEMGISVSPAKAAPWFRLHD
                     AAFHSPVPGLVDQANPNLQSLLGVVFQKISTPPPSAGSALGKLLAVLADPGKIIVPTT
                     SGGLAVSADSLAALNADPLG"
BASE COUNT          325 a          660 c          667 g          455 t
ORIGIN      
        1 tcgcgttctc gctcgcaccc atccctgcga gcgggagcgt tgccggagtt tcggtttctg
       61 ccgatgcgct cgagattacg gtcgattatg cgctgggcgc cgccatcgac gcacgcgctt
      121 cgatcaacgg gatcaaagtc actgcgggcg ccgattcggt atcgattccc tcgctgactt
      181 tcccgccacc atcggggttc acgattacgc ccgatctcga aaaggcattt cgcctgctct
      241 tttcgatggc gatcgcctcg tgggttcctg cgcagggatc gggcctggct gcgctgctcg
      301 gcctgaccgg cgggtacttt ggcttaccgg ctgactggcc gttgctggga ccaggatttc
      361 tcgccgatcc gttgggtgcg ctgcgagcgt ggctgggaca catcgccgtc gatgtgtccg
      421 cggaccagac gccgttgctg cccaaagcgc ttgaagtgtt gccatcggtt tttggaagca
      481 gcactggcgt gttgtccggt tcgggtatct atgacgatcc atgggctgtc gcgatcgata
      541 ccggcgttga actgctgacg tggtttgagc cgaatggtcc tccgtcgaac tggcttgcac
      601 ctctgtcggg agcgattacg gctgcggctt cgttcaatga actgttcctc gcggctcagg
      661 ggcttggctc tttcgtgtcc ggcgtgcagg aggcgtgcag tacaccgagt gcaacgtggg
      721 caacagggtt caagtcgctt gcaacatggc tggcaggaac cgatggcttc gtgccgctgg
      781 agtcgcaggt tcctgccacg gctcaatgga cggcgggcac cacgctgcag tcctcgcaca
      841 tgaaaatgcc cagcgatgct gcggccatca cgcagatcaa tgctcagatc gcaagctgga
      901 gtccgggagc caaggttgtt ctgctgctgg gcccaagctt ttccgaccac agcatctggg
      961 atacgtttct gtccgctgtt cctctcaagc cgaattttaa ttttcgcgtg cccggcatcg
     1021 atccggcgaa tgtcgatctg ctcagcgtta cagatgccgc ggatttctac acggtcgacc
     1081 tgatcgacaa cggccttgcc ggacttgccg cgcagatcgg ccgtgtggtc gagcggatcg
     1141 ggcagcttcg tcccggcgtg aaggtgacgc tggtcgcgca ctccacggct ggaatcgcgg
     1201 cgcgcagtta tgcggccgtg aacgcggccg ccgtgcaggg actgatcacg cttggcacgc
     1261 cgcaccttgg atcaacgctg cttccgatca ccgatcagcc tacaggctcg gcgctgggag
     1321 tgctgaacac gctggttcct tccctgcccg cgggttcgct gaacgatgcc gtggccgcgc
     1381 tgacgctcgc tgccgatggc tatgcgcccg cttccggtgc agggcaactg cccgtgccgg
     1441 cgcctttccc ggtagcggct tttgcggatc ccggatcgac ggaaacggga ggagtgccgg
     1501 cgctcgccat cggcagttcg ctgaatgcgg acttgctcgc ggcggtaaag accggtgtgg
     1561 cttcgctcgc tggaggggtc tcgaatccag cgccgactca tcttgctttc ggaatccgaa
     1621 gccgtgtgga tttcggcacg gatggcgatg ttcaggtcga cgcctttgtg cgcgccgatg
     1681 caggccggtt tgcgctctcc acagacgcgg cggaacccgc ccgacccgcc catgcactca
     1741 acgcttctgt cactctgacg cggcctgacg actggctagc cggttctcct tcatcgcccg
     1801 agcgcgtgcg ctggatggag atgggaatta gcgtctcgcc agccaaagca gctccatggt
     1861 tccggctgca cgacgcggcg tttcactctc ccgttccagg tctcgtcgat caggcaaatc
     1921 cgaatctgca gtcgcttctg ggtgtcgtct tccaaaagat ttcaacgcct ccgccgagcg
     1981 ccgggagcgc attgggcaaa ctgctcgcgg tgctcgccga tccaggcaag atcattgtgc
     2041 ccacaaccag cggaggcctc gcagtctccg cggattcgct ggcggcgctc aatgccgatc
     2101 cgctggg
//
LOCUS       DASZYN010000172         3668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_968593, whole genome shotgun sequence.
ACCESSION   DASZYN010000172 DASZYN010000000
VERSION     DASZYN010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968593"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /gene="ruvB"
                     /locus_tag="VHE33_04355"
     CDS             <1..610
                     /gene="ruvB"
                     /locus_tag="VHE33_04355"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HVT96714.1"
                     /translation="IGQGPAARTHVMEIKPFTFVAATTRPGLLSSPLRSRFGILLRLE
                     FYTDEDLRYIVTRSAEVLGIPIDQDGAAEIAMRSRGTPRIANRLLRRVRDFAQVRASG
                     NIDRKTACDALQLLEVDAHGFDELDRRLLMTIIEKYDGGPVGLGTLAATLAEEEDALE
                     EVYEPFLIQIGFLDRTPRGRVATRLAYEHFGLPMPRGQRGLF"
     gene            1441..2559
                     /locus_tag="VHE33_04360"
     CDS             1441..2559
                     /locus_tag="VHE33_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HVT96715.1"
                     /translation="MLGIDATAARRAWSAILVLLLVLGIFAIRYTLLVFIVAILFAYL
                     LCPLVEFNQRRIFPRSRTMAALFPFAVFIITVTAAAVIVHPQFVKERDQLFAQVSSPQ
                     FKQHLQEWSFDGIPVGRLIFTNYGTLLTMLPKDKLGDSLHTTIRDAGSLILVPILAFF
                     FLRDGRHIRDTLVALLYGSERSTPLVRKGKTVCISLLCDAHALILDYMRALLLQCACT
                     FVACAVFLTLLHVPYAILLALIAFPLEFVPLVGPLISATLILAVCEFSLAVNPASVPH
                     LHIAWVIAFLAVYRVFTDYVLSPIIMRRGVQLHPLLIIFGVYAGGELGGVGGIFLSVP
                     LLALARLVFFEWRKPVPVVEVEEMVLAPDVDPELDAVG"
     gene            complement(2952..>3668)
                     /locus_tag="VHE33_04365"
     CDS             complement(2952..>3668)
                     /locus_tag="VHE33_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96716.1"
                     /translation="PAFEPASSDRGGSMRPPVLIAVTIGLTMLCSVHASAQKPDLHAG
                     GAQPSEIPNHETTEVLLPGYNLTGAKVTTDPICHIVSQVATDREIRMKIEGTRKIDDP
                     DDKCSLFVTTPKGKAATWIVVDLTDEESEQQKTQERATETAKGAEFVARSGKSWKLIF
                     AGGGKATYTVSDEVDDGMPLFKSSGGGTAKIMVSNDGTVSILETGCIRTGKVTGNTVK
                     NGQSLGTCSPPGAWTGVVER"
BASE COUNT          596 a         1049 c         1183 g          840 t
ORIGIN      
        1 catcggccag gggccggcgg cgcggacgca cgtgatggag atcaagccgt tcacgtttgt
       61 agccgcgacg acccggcctg gactgctgag ctcaccgctg cgttcgcggt ttgggattct
      121 gctgcggctc gagttctata cggatgagga tcttcgctac atcgtgacgc gctcggccga
      181 agtgctgggc atcccgatcg atcaggacgg agcagcggag atcgccatgc gcagccgggg
      241 gacgccgaga attgcgaatc ggctgttgcg gcgggttcgg gatttcgcgc aggtacgggc
      301 gagcgggaac atcgatcgca agaccgcgtg cgacgctctg cagttactcg aagtcgatgc
      361 gcacggcttc gacgagttag atcgccgctt actcatgacc attattgaga agtacgatgg
      421 cggcccggtt gggttaggga cgttagcggc gacgctggcg gaagaagagg acgcgctgga
      481 agaggtttac gaaccgtttc tgatccagat tggttttttg gatcggacgc cgagggggcg
      541 ggtggctacg aggctggcgt atgagcattt tggattgcct atgcctcggg ggcagcgggg
      601 attgttctga cggtcgtgct cgcgctcaga tccacaatcg gatccgggga aagaaaagca
      661 ggtccattcg actcgcgtct gaattgattc agcgcgcaaa ggcgcgcgct gatatgcgga
      721 tgcttcgctc aggatgacag agttttggct tctggcgaga gcggagtgag gctaccgggc
      781 gctttcgacg ccgagacaat gtacatccgc tgttttttcg aggccctttt agatctaatt
      841 gattcggcat ggcttagcgt agttaacaag tttgaaaaca tgctaagttg ctgaaaaggg
      901 gatacttcga tacggccgct tgggttacac acacggccat gtttgtcata cacgtggcag
      961 tttgtgttat cgaggcggcc gagtttgtaa ccgaagcggc cgcgatcgag tcacgtgcgg
     1021 atgccgggtt ttcaaagaac gctcttgcgg tccggcctgg gccggtgtta agggtacagg
     1081 gtgcagggta cagggtgcag atcactgatt gcggatcgtg ccgtcagctg gatcggtggt
     1141 tggtctcttc ttctaaaaca aaaaagctct gctcgcggcg atcgcggaca gagctttgct
     1201 tgttcttagg gtaggaattt tcgtgtatta aggcaaggaa attcaataag ccgctgggta
     1261 cggaggtacc gtggcggcgg aatcgggaac tcttcgcgga aacggggtgt tttccccgag
     1321 aggcccgttc gttttccagg gaagcctgtt ttcgcttgtc cctcacaatc ggcgtgggct
     1381 aaactgcccc aaccccatcc cctctggccc cctccagaat cccagggcgg agtgttcctc
     1441 atgctgggca ttgatgcaac ggccgcgcgg cgcgcctggt cggccatcct ggttctgctc
     1501 ctggttctcg gcatctttgc gatccgatac acgctgctgg tcttcatcgt ggcgatcctg
     1561 ttcgcctacc tgctgtgccc gctggtcgag ttcaatcagc ggcgcatctt ccccaggtcc
     1621 cggacgatgg ccgcgctctt tccgttcgcg gtgttcatca tcaccgtcac ggctgccgcg
     1681 gtcatcgtgc atccgcagtt cgtgaaggag cgcgaccagc tgttcgcgca ggtgagcagt
     1741 ccgcagttca agcagcatct gcaggagtgg agcttcgacg ggattccggt aggccggctg
     1801 atctttacca actacggcac gctgctcacg atgctgccga aggacaagct cggcgacagc
     1861 ctgcacacca cgattcgcga cgccggcagc ctgatcctgg tgccgattct cgcgttcttt
     1921 ttcctgcgcg acgggcggca catccgcgac acgctcgtgg ctctgctcta tgggagcgaa
     1981 cggagcacgc cgctggtgcg caaggggaag acggtgtgca tttcgctgct gtgcgatgcg
     2041 cacgcgctga tcctcgatta catgcgcgcg ctgttgctgc agtgcgcgtg cacgttcgtg
     2101 gcctgcgctg tgttcctgac actgctgcac gtgccttacg cgattctgct ggcgctgatc
     2161 gcgttcccgc tggagtttgt gccgctggtg ggcccgctga tctcggccac gctcattttg
     2221 gcggtgtgcg aattcagcct cgcggtgaac ccggcgagcg ttcctcacct gcacatcgcg
     2281 tgggtgattg cgtttcttgc tgtgtaccgg gtcttcacgg actacgtgct ttcgccgatc
     2341 atcatgcggc gaggcgttca gttgcatccg ctgctgatca tttttggtgt ttacgcgggc
     2401 ggcgaactgg gcggggtggg cgggatcttc ctgagcgtac cgcttctggc gctggcgcgg
     2461 ttggtgttct ttgagtggcg gaagccggtg ccggtcgtcg aggtggagga aatggtcctt
     2521 gctcccgacg tggatccgga actggacgcg gtaggctgag cctccgattg tccggtaagg
     2581 gacggtcgcg tgaatcgttc caattccggg cctgaaggcc cgcatctgcg gctgcctttt
     2641 ccccggcctg aaggctccat tcgactcgcc cgcttgcgcg actcgctcag gacaggctta
     2701 aggcttctac cgtcgtgccg ctgacgcgcc accgcttcgc gactgggcac ggctcacccg
     2761 gttggagccc cctgtggcgt gtcatcgaca gagcgtacta gtttaccgcc ggtgtcgaag
     2821 gcttggtggc ggcgaatgcc agaacggccg caatcacgat caccacgccc aggagaattc
     2881 gcaggatcat cgttcctcct ggtgctgctt aaaagatggc ccgactgcga atttgcgtcg
     2941 tccggcttgc gctaccgctc gaccacgccc gtccacgctc cgggcggaga gcaggtcccc
     3001 agcgactgcc cgttcttcac ggtgtttcct gtgactttcc cggttcggat acacccggtt
     3061 tcgagaatcg ataccgtacc gtcgttgctg accatgatct tggccgtgcc gccgccgctt
     3121 gatttgaaca ggggcatccc gtcgtcgact tcgtcgctta ctgtgtaggt cgctttgccg
     3181 ccgccggcga agatcagctt ccagctcttg ccggaacgcg ccacgaactc cgcacctttg
     3241 gccgtctccg tcgcgcgttc ctgggtcttc tgctgttcgg attcctcgtc ggtcaggtcg
     3301 acgacgatcc acgtggcggc cttgcccttc ggcgtcgtca cgaacaagct gcacttgtca
     3361 tccgggtcgt cgatcttgcg cgtgccttcg atcttcatgc gaatctcgcg gtcggtggcc
     3421 acctgggaga cgatgtggca gatgggatcg gtggtcactt tggcgccggt caggttgtaa
     3481 ccgggaagga ggacttcggt cgtttcgtga ttgggaatct cggagggctg cgcgccgccg
     3541 gcatgcagat cgggcttctg cgcgcttgcg tgaacagagc acagcatcgt cagcccgatg
     3601 gtcactgcga taagaactgg tggacgcatc gatcctccgc gatccgagct tgcaggttcg
     3661 aaagctgg
//
LOCUS       DASZYN010000173         3809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_327052, whole genome shotgun sequence.
ACCESSION   DASZYN010000173 DASZYN010000000
VERSION     DASZYN010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327052"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2553)
                     /locus_tag="VHE33_04370"
     CDS             complement(<1..2553)
                     /locus_tag="VHE33_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVT96717.1"
                     /translation="MSAGDEIPGDVALIREKQSLPWSVAARIAWRELRAGKAKFMFVL
                     LSVAIGVAALTGVRGFSTSFARTLFLQARSIMAADMSARMFRQATPNETRQLDALASP
                     QSGLDVRRTVVTETVSMAMVEGDPVPLLVTLKAIDPRQYPFYGTVLLSTGQSLRDALT
                     NDTVVVDDNLLVRLRAKIGDRLKLGGRWFRIAAVIQKEPDRMTAGVGLGPRVMMTRQA
                     MEETELLQPGSRATERYLFALGDKLKVADVRAQIEKILPEAQVTDFRETSPALTQGVD
                     RATGLLSLICLVAMVLGAIGVAMAMRAHLQQRIEILAVMKSIGARSSDILRIYLIQTL
                     LLGIGGALIGVALGMGVEYAFPTLLGKLLPLRPEIRLPLNPVLAALGTGVLTTLLFCL
                     PPLLDVRRVRPSLVLRRMVESGDGSGWIGWLKEHKLQLASVFVILGGLGGIAAALADS
                     ALVGKWFAICLAGLLVFILGLSATTLRGLRAFLSRTRLHLHSALRHGLANLYRPGNQS
                     TAVLAALGTGVMLILSVFLMQRSIVNALHEDLKPDTPNLFLVDMSSKEMPGIRAELKK
                     QPGVIGELEAIPIVSARIGSIDGTPVDQLRENKEYPKHLLQTATLSWADAAPKGTKIV
                     EGKWWSGNGGDDLAVMDFIAKRLKLKVGSKMTFIAGEKEIPVRVAVIYKVVGENAFAR
                     SSYILPQALLADQPTVWYGDIHARPSAIPQIERALFATYPTVTIINIADILTTIGDVV
                     DQITLVIRFLAGFSILSGLIILASSVASTRFRRVREVVVLKTLGATRNRIATVFSVEF
                     VVLGLLAGAVGTLFANVLSRILLHRMDVVFQRDVRGSLTAVLGTAVLAVVTGW"
     gene            complement(2550..3266)
                     /locus_tag="VHE33_04375"
     CDS             complement(2550..3266)
                     /locus_tag="VHE33_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT96718.1"
                     /translation="MIEVKGLRKSIRNGARVVEILRGIDLTIPKGQFAAIMGASGSGK
                     STLLGLLAGLDSPSEGEVILDGIRISHLEEDKLAEVRGQRIGFVFQSYQLIPTLTALE
                     NVMLPYELNARGDARKKAEPLLAAVGLQDRLHHYPVQLSGGEQQRVALARAFVLEPPI
                     VLADEPTGNLDSANGQHVLELLGERQRESATTLVLVTHDPQIAERANRRIVLKDGIVV
                     ADVMQGAGQAVVGADAVAAV"
     gene            3453..>3809
                     /locus_tag="VHE33_04380"
     CDS             3453..>3809
                     /locus_tag="VHE33_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HVT96719.1"
                     /translation="MIDCFGDSITAGYGLTPGHAWPDDLQSLLDQRGYHYKVVNSGVS
                     GNTSKDGVDRLKDVLRLHPAIVIVEFGGNDGLRGLPIADTRRNLDTIVSTLVRSGSKV
                     ILAGITLPPNYGSDYIK"
BASE COUNT          762 a         1398 c          946 g          703 t
ORIGIN      
        1 ccaccccgta acgaccgcca gcaccgccgt gcccagcacc gcggtcaacg atccgcgcac
       61 atcgcgctgg aacacaacat ccatccgatg cagcagaatc cgcgacagca cgttcgcgaa
      121 cagcgttccg accgcgccag ccagcagccc cagcaccaca aattccacac tgaatacggt
      181 cgcaatacgg ttccgcgttg cgcccagcgt cttcagcacc accacttcac gtactcgccg
      241 gaaccgcgtg ctcgccacgc tcgacgccag aataatcaag cccgacagaa tcgagaatcc
      301 cgcaagaaac cggatcacca gcgtgatctg gtccaccacg tcgccgatcg tggtcagaat
      361 gtccgcgata ttgatgatgg tcacggttgg atacgtcgca aacagcgctc gctcgatttg
      421 aggaatcgcg ctcggcctgg catgaatatc gccataccaa accgtcggct ggtccgcgag
      481 cagcgcctgc ggcaaaatat acgaacttct cgcaaacgcg ttctcgccca ctaccttata
      541 gatgacagcc acccttaccg gaatctcctt ctcgccggcg atgaaggtca ttttggatcc
      601 gaccttcagc ttgagtcgct ttgcaatgaa atccatcacg gcaaggtcgt ctccaccgtt
      661 tccggaccac cactttccct ctacgatttt ggttcccttc ggcgccgcgt ccgcccagga
      721 cagcgtcgcg gtctgcagca gatgctttgg atactccttg ttttccctga gctgatccac
      781 cggcgttccg tcaatcgacc cgatgcgcgc cgaaacaatc ggaatcgcct ccagttctcc
      841 aatcaccccc ggctgtttct tcaactccgc gcgaatgccg ggcatttcct tgctgctcat
      901 atcgacgagg aacaggttgg gcgtgtccgg tttcaaatcc tcatgcagtg cattcacaat
      961 cgaccgctgc atgaggaaaa cgctcaaaat gagcatgact ccagttccca gcgccgccag
     1021 caccgccgtg gattgatttc caggccgata cagattcgcc agcccatgcc gcagggccga
     1081 gtgcaaatgt aaccgtgtcc gtgacagaaa cgcccgcaaa ccccgcagcg tcgtcgccga
     1141 caatcccaga atgaacacca gcagccccgc caggcaaatc gcaaaccact tccctaccag
     1201 ggccgaatcg gccagggccg cggcaatgcc tcccagtccg cccaaaatta cgaacaccga
     1261 ggcgagttgc agcttgtgtt ccttcagcca gccaatccat cccgacccgt cgcctgactc
     1321 caccatccgt cgcaacacca ggctcggtcg aacgcgccgc acatccagca gcggcggcag
     1381 acaaaacagc agcgtcgtca gcacgcccgt tcccaatgcc gccagcaccg gattcaacgg
     1441 caggcgaatc tcgggccgca gcggcagcag cttgccgagc agcgtaggaa atgcatactc
     1501 gacccccatc cccagcgcca cgccgatcag cgctccgccg atcccaagca gcagggtctg
     1561 gatcagatag attcgcaaaa tgtccgacga ccgagcccca atcgacttca tgaccgccag
     1621 aatctcgatg cgctgctgca ggtgtgcgcg catcgccatg gcgacgccaa ttgcacccag
     1681 gaccatggct accaggcaga tcaaactcag taaccctgtc gcccgatcca ctccctgggt
     1741 cagcgcgggg ctggtctcgc gaaaatccgt gacctgcgcc tccggcaaaa tcttttcgat
     1801 ttgcgcgcgg acatcggcca ccttcagctt gtcgcccaga gcaaacagat accgttccgt
     1861 ggcgcggctg cccggctgca gcagttcggt ctcctccatc gcctgccgcg tcatcatcac
     1921 gcgcgggccg aggccgacgc cggcagtcat gcggtcgggc tccttctgaa tcacggcggc
     1981 aatccggaac catcgcccgc cgagcttcag tcggtcgccg atcttcgccc gcagacgcac
     2041 caaaagattg tcatccacca cgacggtgtc gttcgtcagc gcatcacgca ggctttgccc
     2101 cgtcgacagc agcacggtcc cgtaaaacgg atactgccgc ggatcaattg ccttgagggt
     2161 gaccagcaga ggcacaggat cgccctcgac cattgccatc gaaaccgtct ctgtgaccac
     2221 ggttcgccgc acatccagcc ccgactgcgg gctcgcgagc gcatccagtt gcctcgtctc
     2281 gttcggtgtg gcctgtcgaa acatccgcgc cgacatatcc gccgccatga tgctgcgcgc
     2341 ctgcaggaac agcgttcggg cgaacgatgt gctgaacccg cgtaccccgg tcagagccgc
     2401 caccccgatg gcgaccgaca gcagcacgaa cataaacttc gctttccctg cccgcagctc
     2461 gcgccaggca atgcgcgccg ccacactcca tggaagagac tgcttctcgc ggatcaacgc
     2521 aacgtcccca gggatttcgt cgcccgcgct cataccgccg ccaccgcatc cgctccaaca
     2581 acagcctgcc cggcaccctg cattacatcg gccacgacga ttccatcttt gagcacaatc
     2641 ctgcgattcg cccgctccgc gatctgtgga tcatgggtca ccagcaccag cgtcgtcgcg
     2701 ctctcgcgct gccgctcgcc cagcagttcc agcacatgct gtccgttcgc ggaatccagg
     2761 tttcccgtcg gctcatcggc cagcacaatc ggcggttcca gaacaaacgc ccgcgccagc
     2821 gcgacccgct gctgctcccc acctgacagc tgcaccggat agtgatgcag ccggtcctgc
     2881 aaccccacgg ccgccagcag cggctcagcc ttcttccgcg catcgcctct tgcattgagc
     2941 tcgtacggca gcatgacgtt ttcgagcgcc gtcagcgtgg gaatcagctg gtacgactga
     3001 aagacaaatc caatccgctg cccccgcact tctgccagct tgtcctcttc cagatggctg
     3061 atcctgatgc catcgaggat cacttctccc tccgacggcg aatccagccc ggcaagcagg
     3121 cccagcagcg tactttttcc gctgccgctg gcgcccatga tcgcggcaaa ttgccccttc
     3181 ggaatcgtga ggtcgatccc ccgcaaaatc tccaccacac gcgccccgtt ccgaatcgac
     3241 ttcctgagcc ctttgacttc gatcatccac tctcctctgc ggcttccttc cattgtatcc
     3301 ctatgtagca tacttccttc caaatccagc cctgccgcca gccgtcccgt tgctagacta
     3361 cccatgtgtt ccgccgcccc atcctcctgg ctttcctttg cgcgctctcc ctcaacacat
     3421 acgccgtgcc gcctcgtccc gttcccgttc ctgtgattga ctgtttcggg gacagcatca
     3481 ccgccggcta tggactcacc cccgggcacg cctggcccga cgatctgcag tctctcctcg
     3541 atcaacgggg ctaccactac aaagtcgtga actccggcgt cagcgggaac accagcaaag
     3601 acggcgtcga ccgactgaaa gatgttctcc gccttcaccc cgcgatcgtg attgtggaat
     3661 ttggcggcaa cgacggcctg cgcggtttgc ccatcgcgga tacccgccgc aaccttgaca
     3721 ccatcgtctc caccctggtt cgctccggca gcaaggtgat cctggccggc atcacgctgc
     3781 ctcccaacta cggttccgac tacatcaag
//
LOCUS       DASZYN010000174         1739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_583363, whole genome shotgun sequence.
ACCESSION   DASZYN010000174 DASZYN010000000
VERSION     DASZYN010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583363"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            396..1256
                     /locus_tag="VHE33_04385"
     CDS             396..1256
                     /locus_tag="VHE33_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96720.1"
                     /translation="MTLGIAVPFPGGSILCTDSPIEANDGSATESEAALMSVTSGKRI
                     FAITYAAKDPRAGRMLASDIATVACSAPTRQDIVPGIKRVMGDWFRSYGVAQIPALQF
                     LVASNSTDDNAGRILLCEPPSTVVETFGPCVVGAAAGPITPWLKMLTARQNQSISLRS
                     IVLRLTYLLYLARKYDPGVGEDTEMIVLSNRGSFSYIDRREIRQAEGLGEKVDMLLLD
                     VARQLLTAEMEQNPQTIAEEFLRGYFDVMEQNPQVYFPSLAWLDKMTGNGGSAGPIPV
                     PVPMPTGTRG"
     gene            1415..>1739
                     /locus_tag="VHE33_04390"
     CDS             1415..>1739
                     /locus_tag="VHE33_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96721.1"
                     /translation="MGDFLSDLRYSLRMLISNPAFTITAVAALALGIGANTAIFTVVN
                     TVLLKPLSYPDPDRIVQLMNTSPDGNFGVASPTKFNNWRAQTSVLQDVSAYDFGGPGF
                     NLTGEV"
BASE COUNT          351 a          597 c          443 g          348 t
ORIGIN      
        1 cccgcagctg ccgtaattct ctcggacgtc gatgccttca ccatgtctac gctgtatacc
       61 agatgaatgt atacgctgta tacccggaca aagttcatcc tgctgaaccg cagcactcgc
      121 gccgcaattc cctgcgctcc acgctgcccg tcaccctgcg atgttgaacg cgttggttct
      181 caattcgaaa tattgcgagc catttcattg acccttcccg gcttcgcgta tcgaatgaaa
      241 ggcagggctt actgccgtca ctcggagacg gcgcacgacc cgacctcatc ggcagcgctt
      301 cgcggagagc cgaacgtaaa ccggaccagg acaagagccg cgcctaagcg gcctgctttg
      361 tccagtgaat taaagaatgc gaaaggcccg caggcatgac cctagggata gcggtcccgt
      421 ttccaggcgg atcgatcctc tgtaccgatt ctccgattga ggcaaacgac ggctcggcga
      481 ccgaaagtga agccgccctc atgtccgtca cctccggcaa acgcatcttt gccatcactt
      541 acgccgcaaa agatccccgt gcaggacgca tgctcgcttc tgacatcgcg acggttgcct
      601 gcagcgctcc cacccgccag gacatcgtgc ccggcatcaa gcgggtcatg ggtgactggt
      661 tccgttccta tggcgtggca caaattcccg cgctccagtt cctcgttgcc agcaactcca
      721 ccgacgataa cgccggacgc attctgctct gcgagccgcc atccacggtc gtagagacct
      781 ttggaccctg cgttgtgggt gccgcggccg gtcccatcac cccatggctc aagatgctca
      841 ccgccagaca aaatcagagc atcagtttgc gctccatcgt ccttcgcctg acttatctgc
      901 tttacctcgc acgcaaatac gaccccggcg tcggcgagga caccgagatg atcgtccttt
      961 ccaatcgtgg ctccttcagc tacatcgatc gcagggagat tcgccaggcc gaaggtctcg
     1021 gcgaaaaagt cgacatgctg ctgctcgacg tggcgcgaca gctgctgacg gcggagatgg
     1081 aacagaaccc ccagaccatc gccgaggaat tcctgcgcgg gtatttcgat gtcatggagc
     1141 aaaaccctca ggtgtacttc ccctccctgg cctggctgga caagatgacc ggcaacggcg
     1201 gctcggcggg cccgatcccg gtgcctgttc ccatgccgac cggtacacgc ggctaaccct
     1261 ctccatcgcg ttttcagaac agctcaccct cgccgcgcct ccgcgtcaaa gtgttaccac
     1321 tcgtaacatt tttgccactg atctattgca gaaagtgtag attggcaggg accattttcc
     1381 tgtagcggac ccagatccaa aggcggcaaa caccatggga gacttcctca gcgacctgcg
     1441 ctactccctg cgcatgctca tctcgaaccc ggccttcacc atcaccgcgg tcgccgccct
     1501 cgcccttggc atcggtgcca acaccgccat cttcaccgtc gtgaacacgg tgctcctcaa
     1561 gccgctctcc tatccagatc ccgaccgcat cgtgcagctg atgaacacat cgccggacgg
     1621 caacttcggc gtcgcttccc cgaccaaatt caacaactgg cgcgcgcaaa ccagcgttct
     1681 gcaggatgtc tccgcgtatg acttcggcgg tcccggcttc aatctcaccg gcgaagtcc
//
LOCUS       DASZYN010000175        11278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1099833, whole genome shotgun sequence.
ACCESSION   DASZYN010000175 DASZYN010000000
VERSION     DASZYN010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 11278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11278
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099833"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VHE33_04395"
     CDS             <1..615
                     /locus_tag="VHE33_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96722.1"
                     /translation="LRHGAPYLTPFASAEQLRLFFAQNPTAQRIPNFFLFSSAIPFGI
                     FATTAVSRLRFLGVRAAGANIALFGGISAAIALFFSGMAGSILSVPDVLASAEVVKAV
                     FYLSFLSGGVFYAVSFGLLAAGISVTSYFKGLLPRWIAALGIAVALAGELSSLSLIAY
                     PANFFIPVARFAGFLWMLLAAFALMRVPPSLQSASAQPGGISVA"
     gene            643..1308
                     /locus_tag="VHE33_04400"
     CDS             643..1308
                     /locus_tag="VHE33_04400"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HVT96723.1"
                     /translation="MPAQQTNSSGSPRAKPVPLTLRIGFWICIIIAVAAVIRRLAALA
                     HPSSSGPPQMAALDTVFASHAALTLAHIVPALAFVLVTPFAILPRFTRLAWPERLLFP
                     LGTIVGITAYAMSVYAVGGWTERSAVLFFNSLFLFSIVRAWYYWIHSQSALERQWLLR
                     AVVVLLGIATTRPVMGIFFATSRMTHLTPHQFFGIAFWIGFSINWLVVEWWLRTTGKR
                     VPA"
     gene            complement(1347..1808)
                     /locus_tag="VHE33_04405"
     CDS             complement(1347..1808)
                     /locus_tag="VHE33_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVT96724.1"
                     /translation="MLSQIGGRSAQEFARLLGPLQLTPPDAGILRIVWEHAGISQQNL
                     AKALRMHPSRLVGVIDALEKRGLIVREPNVSDRRLYSLRLTAQGEAVFRSIGELAREH
                     NKLICTGLNSAECAQLESLLQKIAGAQGLAPGVHPGYRELGKARRRTGKSD"
     gene            complement(1875..3491)
                     /locus_tag="VHE33_04410"
     CDS             complement(1875..3491)
                     /locus_tag="VHE33_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HVT96725.1"
                     /translation="MSAAVDPIELAIFRSAVHSVAEEMGAALRRTALSPNIKERRDYS
                     CAVFDREARVIAMGDHMPVHLGSMPMSVEAAVAATQFAPGDIVVLNDPYAGGTHLPDI
                     TMVLPVFLPEGERPEFYVSARAHHADVGGMFPGSMGPAREIYQEGLRIPPVRIVRGGK
                     MDRDVQNLILMNVRTPAEREGDLAAQMGACRVGERRILELVEKYGPERLRELADELLD
                     YSERLVRAELRRMPPGEFAADDWLDDDGVDDEPIRIAVKMQIDPILATMHVDFSASSE
                     QVTGSVNAVRAITLSACFYVLRCLLEHDAPATAGILRPLTLVTQPGTVVDARPPAPVA
                     GGNVETSQRIVDVLMRALALAVPDRVPAASAGTMSNLTIGGIDPRTGQPFTYYETMAG
                     GMGARPGIEGISGVQTHMTNSLNTPIEALEYAYPFRVRRYEYRRGSGGEGKFRGGDGL
                     VREIELLGPAQATLLAERRKFRPWGLAGGNEGAAGRATVKRANTGELTELAGKCSVYL
                     EAGDVLRIETPGGGGWGRRDSALIVQELND"
     gene            complement(3488..5551)
                     /locus_tag="VHE33_04415"
     CDS             complement(3488..5551)
                     /locus_tag="VHE33_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HVT96726.1"
                     /translation="MQEYGWSQPRCCPTTIREHGPLRTAIDTGGTFTDCVSLRNGELR
                     VLKVFSTPADPAGAVLQALAQAGQGEAVDVRHGTTLGTNAMLERRGARVAFVTTAGCE
                     DTIAIGRQTRAALYDWFAPVPVCLVPSELRFGVEERVSAEGRILREPNEAELQKLVQA
                     IRESGAEAVAVSLLFSFANAQTERRIETAMRELRIPVSVSHRILPEFREYERAATTVV
                     NAYLAPLMRGYLLHLEERVRAEHGGTGVDVMQSSGGIVSARLAAEEPVRTVLSGPAGG
                     VIGACRVAAWAGFDRIIGLDMGGTSTDVFLSDATRGGVQRTRDSVVAGVPVSVPMLEI
                     HTVGAGGGSIARFDEGGMLRVGPESAGADPGPICFGRGADSAMRATVTDANLLLGRLD
                     AESFLGGSVPLHRDRARKLLEEQKGPLASVEDFAAGIVRVVETEMDKAIRVISVEQGY
                     DPRDFTLVAFGGGGPVHACALARALRIPKVLVPAMPGALSALGILLADVVRDHARTVM
                     LAEDAIESLAPMFRELEEQGRKEFAAEGLSGSAEFSLDMRYTGQGYELNVPWDSIAPM
                     ESIAVFHRLHRQRYGFCDEEKQVQVVNLRLRMKAETEPYVPRRRELRSGTGRQACYVE
                     RQVCFDGKFVQSRFYRREELVPGDVIPGPAMITEYTSATVLPADCSAAVDGFGNLVIT
                     IGEDA"
     gene            5571..6800
                     /locus_tag="VHE33_04420"
     CDS             5571..6800
                     /locus_tag="VHE33_04420"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT96727.1"
                     /translation="MNGFSAWLTEASAEQKRTLLAASLGWMLDSMDVMLYALVLGQVQ
                     RELHLSAAMSGAMMSMTLLSAAIGGIAFGWFADRAGRVRALSASILIYSVATFLCGLT
                     HTALALLTCRIILGLGMGGEWASGAALVAETWPAHHRGKALALMQSSWAVGYALGAAL
                     VAVIMPHFGWRAVFFFGTAPALAALWIQSRLHEPEAWRQEHTARPRWGQLFRGRLGFS
                     TLVCATMNAASLFAWWGLFTWVPRFLSMPISQGGRGLGIVMTSEWTIVMQIGTFLGYV
                     SFGYIADRFSHKYTYIAYLLIAAVMVPIFASVTSPSWLLIVGPLVGFFGTGYFSGFAI
                     LTSELFPTVLRASAMGFVYNIGRVLSAVSPWLIGRVSERSGLGFALTLTSAGFLLAAL
                     IASALRSPAPSIAVTDP"
     gene            6871..8025
                     /gene="bshA"
                     /locus_tag="VHE33_04425"
     CDS             6871..8025
                     /gene="bshA"
                     /locus_tag="VHE33_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HVT96728.1"
                     /translation="MKIGITCYPTYGGSGVVATELGIELAAAGHQVHFIAYSQPFRLR
                     GREQDIAFHEVPVSNYPLFEYPPYDLALATRMAEVAAYYELDLLHVHYAIPHSISAYL
                     ARQMLLRQNRRLPFITTLHGTDITLVGLDHSYLPITRFGIDESDGVTAISNYLREKTI
                     QEFHPERPIHVIPNFVNCDVYMPLDEDARRAGRARYAEPGEKILVHLSNFRPVKRTAD
                     VIRIFSRVARELPARLLMIGDGPDRSAAEWIAHRKGIQDRVHFLGKQSSVTDILPLAD
                     LLLMPSELESFGLVALEAMACRVPTIATQVGGIPELIEDGVTGRLFPVGDVEGMAGAA
                     VELLSDSSRYLAMAESARRTAKSRYCASKIIPLYESFYQEILSGSATSGV"
     gene            complement(8022..10391)
                     /locus_tag="VHE33_04430"
     CDS             complement(8022..10391)
                     /locus_tag="VHE33_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HVT96729.1"
                     /translation="MSSFSIPLTGLKASSTQLNTIANNLSNMNTTAFKSQSVTFSDLF
                     YQQIGTTGSGDPQQVGAGTRVAATVTDFSQGGINSTGNASDLYINGNGFFVVQDNNGT
                     TELTRDGNFTISGGCLVTQDGQNVMGYPAANGAVNTSAQLAPIQLPVGQVENARATTS
                     MSMTANLNASDAVGTTVLGQVPIYDSLGNPHEATVYFTKTGTNTWSYNFSLDPKPLTD
                     TSSATTRTIADTVSATTRTLADTVSATTRTVTTSASGGNTTIPFDAGTTVDPTTSFTI
                     DDGSTTVTAPAATNGESLSTYLTALQTALTNGGSTATASLTGNTLTITGGTITGSVVE
                     GGVTRNSFTLNSGATVDPTTSFTINDGTTTVTAPAATSGESLATYLTALQTALTNAGS
                     TATASVSGSTLTITGGTITGNVVEGSIAQNTFALDRGGTMDPSTSFTINDGVTTVTAP
                     AATSGESLSTYLTALQTALTNAGSTATASLNGSTLTVTGGAITGTVVEGGITTYAYNI
                     DAGTTIDPSTSLTITGTNGGGNSTTITAPPIAPGETLNSYATALTAAIGNANIQGVVV
                     SVNGNGLSISGPGISLGGNLLESAAGTASGNATGSLTFDTNGNLIAPSTNVVGIQLGG
                     LTDGASNLSLTWDLFGASGAGLITQSAVGTSASSNPVTGTIQDGYASGSYQGFSVDSS
                     GVVSATFSNGKTQVVGQVALANVANLQGLTIEAGNNYATTTASGPATVGIAGTGGLGT
                     LQEKALEGANVDIATQFANLIVAQQAFQASSKAITTFDTISQDTINMIH"
     gene            complement(10418..10960)
                     /locus_tag="VHE33_04435"
     CDS             complement(10418..10960)
                     /locus_tag="VHE33_04435"
                     /inference="COORDINATES: protein motif:HMM:NF015896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HVT96730.1"
                     /translation="MQIFPDTIGAASPLAAQAQDAGSPSGSGSSSTNTATVTANDFLQ
                     LLIAEMKNQDPTANSDPTKFIDQLVQVNSLQQLVEINQDLGGGTSDVTTPSGDAANRT
                     GIESKRPATAVAGGNLSAGDPGSSAAAAVRVASALGRSTETPGASATGMPAGNPFDAI
                     SAAMHGRASAIPTTTTSPAR"
     gene            complement(10985..>11278)
                     /locus_tag="VHE33_04440"
     CDS             complement(10985..>11278)
                     /locus_tag="VHE33_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96731.1"
                     /translation="TAGQVSATLASGTAEAHHAIAAQLPAVREFLAGEHVRIDALSSE
                     RFSSSTGGQGRSSGDPAGNDGRTPVRSPQQERPAQPSPVEMDREGMSYISVRV"
BASE COUNT         1899 a         4055 c         2974 g         2350 t
ORIGIN      
        1 ctccgccatg gagcgccgta cctcacccca tttgcctccg ccgaacaact ccgtctgttc
       61 ttcgctcaga acccgactgc gcaacgcatc cccaacttct tcctcttctc ctccgctatc
      121 ccctttggga tttttgccac caccgccgtc agccgactcc gctttctcgg agtcagggca
      181 gccggagcca acatcgccct gttcggcggc atctccgcgg ccatcgccct gtttttctcc
      241 ggcatggccg gctccatcct gtctgtcccc gacgtcctcg catcggccga ggtcgtcaaa
      301 gcagtctttt atctcagttt cctttccggc ggagtcttct acgcagtctc cttcggcttg
      361 ctcgccgccg gtatttccgt caccagttac ttcaagggcc tcctgccgag atggatcgcg
      421 gcactcggca tcgccgtcgc ccttgccgga gaattgtctt ctctcagcct gatcgcttac
      481 ccggcgaact tcttcattcc cgtcgctcgg ttcgccggct tcctctggat gctcctcgct
      541 gcgtttgcgc tgatgcgagt tcccccttcg ctgcaaagcg cctctgccca acctggcggg
      601 atcagcgtcg cttaaaccgg cgttccaaaa tggaggacgt ctatgccggc gcaacaaacc
      661 aatagctcag gctccccacg cgctaaaccg gtgccgctca ctctccgcat cggcttctgg
      721 atctgcatca ttatcgccgt cgccgctgtc atccggcgtc tcgcggcgct cgctcatcca
      781 tcgtcctccg gaccaccgca gatggcagcg ctcgacaccg tctttgcctc acacgccgca
      841 ctcacgctcg cgcatatcgt acccgccctg gccttcgtgc tcgtcacgcc cttcgcaatc
      901 ctgccgcgct ttacacgcct cgcctggccg gaacgcctcc tcttcccgct cggcaccatc
      961 gtcggcatca ctgcttatgc catgagcgtg tacgccgttg ggggctggac tgagcgcagc
     1021 gccgtcctgt tcttcaacag cctctttctg ttttcaatcg tccgcgcctg gtattactgg
     1081 atccacagcc agtcagcgct cgagaggcag tggctcctgc gcgccgtcgt agttttgctg
     1141 ggaatcgcca caacgcggcc cgtcatgggc atctttttcg caaccagccg catgacccac
     1201 ctgactcccc atcagttttt cggaatcgcc ttctggatcg gcttttctat taactggctg
     1261 gtcgtcgaat ggtggctgcg cacgactggc aagcgagtcc cggcatgatc tctcctcccg
     1321 ggtgcagtca ccgacggaaa acgccgtcag tcagatttgc cagttcgccg cctcgctttg
     1381 ccgagttcgc ggtaacccgg atgcacgccc ggagcgagtc cctgagcacc tgcaatcttc
     1441 tgcaacaacg actccagctg cgcacattct gccgagttca gcccggtgca tatcaatttg
     1501 ttatgctccc gcgccagctc tccgatggag cgaaagacgg cctctccctg cgcggtcagg
     1561 cgcaaggagt agaggcggcg gtcactgaca ttcggctcac gcacgatcag cccgcgtttc
     1621 tccagggcat cgatcactcc aaccagccgg ctcggatgca tccgcagggc tttcgccaga
     1681 ttctgctggc tgatccctgc atgctcccac acgatccgca gaataccggc gtctggtggc
     1741 gtcagctgca acggacccag cagccgggca aattcctgcg ccgaacgccc cccaatttga
     1801 gacagcaaaa aggctacctg tgggggcttg ttcctctgag acttatcgtc gctcacgcat
     1861 cacgattata tcgattaatc gttcaattcc tgaacgatta gagctgagtc ccttcttccc
     1921 catcctccac cgcctggggt ttcgatccgc agcacatcgc cagcctccag atacacgctg
     1981 catttcccgg ccagttccgt cagctcaccc gtattcgccc tcttcacggt cgctcggcct
     2041 gctgcacctt cattgccgcc cgcgagtccc cacggccgga actttcgccg ttccgccagc
     2101 agggtcgcct gagccggacc gagcagctcg atctcccgaa ccagcccatc cccgccccga
     2161 aatttccctt cccccccgga tccccggcga tattcatatc gccgcacgcg aaacggataa
     2221 gcatattcga gcgcctcaat tggcgtgttg agggaattcg tcatatgcgt ctgcactccg
     2281 gagatcccct caattcccgg gcgcgcgccc attccgcccg ccatcgtctc gtagtaagtg
     2341 aatggctgtc ccgtccgcgg atcgatgcca ccgatggtca gattgctcat cgtgcccgcg
     2401 ctcgccgccg gaacccgatc cggcacggca agggcaaggg cgcgcatcag cacatcgacg
     2461 atccgctgcg acgtctccac gtttccaccc gccaccggag ccggcggccg cgcatccaca
     2521 acggttcccg gttgcgtcac cagcgtcagc ggcctcagaa tgcctgcggt cgcgggcgca
     2581 tcgtgctcca gcaggcaacg caatacatag aagcacgccg acagcgtgat cgcccgcacc
     2641 gcgttcacgc tgcccgttac ctgctccgag gaggcactga aatcgacgtg catcgtcgcc
     2701 aggattggat cgatctgcat cttcactgca atccgaatcg gctcatcgtc gaccccatca
     2761 tcgtccagcc agtcatccgc ggcgaactct cccggcggca tccgccgcaa ctctgcgcgc
     2821 accagccgct ccgaataatc cagcagctca tcggccagct cccgcagccg ttctggacca
     2881 tacttctcca ccagttccaa aattcgccgt tcgccgactc ggcacgcgcc catctgcgct
     2941 gccaggtcgc cttccctctc cgccggtgtc cgcacattca tcaggatcaa attctgcaca
     3001 tcgcgatcca tcttgccgcc acgaacaatc cgcacggggg gaatccgcaa gccctcctgg
     3061 taaatctcac gcgccgggcc catcgatccc ggaaacatgc cacccacatc cgcgtgatga
     3121 gcgcgcgccg atacatagaa ctccggcctc tccccttctg gcagaaacac cggcagcacc
     3181 atggtgatat ccggcagatg cgtgccaccc gcatacggat cgttcagaac cacgatgtct
     3241 cctggcgcaa actgcgtcgc cgccaccgcc gcctcgaccg acatgggcat cgatcccagg
     3301 tgaaccggca tgtgatcgcc catcgcgatt acgcgcgcct cccggtcgaa caccgcgcac
     3361 gagtagtcgc gccgctcctt gatattcggc gacagtgccg tgcgccgcag cgccgcgccc
     3421 atctcctccg ccaccgaatg cacggcgctg cgaaagatcg ccagttcaat cggatcaacc
     3481 gccgcgctca tgcgtcttct ccaatcgtga tcaccaggtt accgaaccca tcgactgcag
     3541 cgctgcaatc cgccggcaga acggtcgccg acgtgtattc ggtgatcatg gcgggtccag
     3601 gaatgacatc tccggggacg agctcttctc gccgatagaa ccgcgactgc acaaatttcc
     3661 cgtcgaagca gacctgccgt tccacataac atgcctgtct tccggtaccc gacctcaact
     3721 ctctccgccg tggcacatac ggctccgtct ccgccttcat ccgcagccgc aaattcacaa
     3781 cctggacttg cttttcctca tcgcagaacc cgtaccgctg ccgatgcaat cgatgaaaaa
     3841 ccgcgatcga ttccatgggt gcaatcgaat cccacgggac attgagttcg tacccttgtc
     3901 ccgtgtagcg catatccagc gaaaactctg ccgagccgct cagcccttcg gcggcaaatt
     3961 ccttccgacc ctgctcttcc aattctcgaa acatcggcgc caaactctcg atggcatcct
     4021 ccgccagcat caccgttcgc gcatgatcgc gcaccacgtc ggccagcaaa atccccaggg
     4081 ccgacagggc tccaggcatc gctggaacca gcaccttcgg aatcctgagc gcccgcgcca
     4141 gagcgcatgc atgcaccggc cctccgccgc caaaggccac cagcgtgaaa tcgcgtgggt
     4201 cgtacccttg ctccacagaa atgacgcgga tcgccttatc catctccgtc tcaaccacac
     4261 gcacaatccc cgccgcaaag tcttccaccg acgccagcgg gcctttttgc tcttccagta
     4321 atttcctggc tcgatctcga tgcaagggca cgctcccgcc gagaaagctc tcagcatcca
     4381 gtcggcccag cagcagattc gcatcggtca ctgttgcccg cattgccgaa tcagccccac
     4441 ggccaaaaca aatcggcccc ggatcggcgc ccgccgattc aggtcccacg cgcagcatgc
     4501 cgccttcgtc gaagcgtgca atcgatccgc caccagcccc caccgtgtgg atctcaagca
     4561 tcggcacgct caccggcact ccggccacta ccgaatctcg cgtgcgctgc actccgccac
     4621 gggtcgcgtc ggacaaaaac acatccgtcg acgtcccgcc catatcgagc ccaataatcc
     4681 tgtcgaagcc cgcccacgcc gccacgcggc acgcgccaat caccccgccc gctggtcctg
     4741 acagaaccgt gcgtaccggc tcctcggctg ccagtcgcgc cgagacaatt cctccggaag
     4801 actgcatcac gtccacaccc gtgccaccgt gctctgcgcg gactcgctct tcgagatgca
     4861 gcaaataccc ccgcatcagt ggcgccagat aggcgttcac cacggtcgtc gcggctcgct
     4921 cgtactcacg aaactccggc agaatccgat gagaaaccga aaccggaatc ctcagttccc
     4981 gcattgccgt ttctatacgt ctctccgtct gcgcattcgc gaacgaaaac agcagcgaca
     5041 ccgcaacagc ctccgcgccg ctctcgcgaa tagcctgcac cagcttctgc aactcggctt
     5101 cgtttggctc gcgcagaatt ctgccttccg cgctcactcg ttcctcgacg ccgaaccgca
     5161 gttcgctcgg cacaaggcaa accgggaccg gcgcgaacca gtcgtacagc gcggctcgcg
     5221 tctgccgccc aatcgcaatc gtgtcttcac agcctgccgt cgttacgaaa gcgacccgag
     5281 cgccgcgccg ttccagcatc gcgttcgtcc ccagcgtggt tccgtgccga acgtcgacgg
     5341 cttcgccttg cccggcctgt gccagcgcct gcagaactgc acccgccgga tccgccggtg
     5401 ttgaaaacac cttcagaacc cggagctctc catttctcag agacacgcag tcggtaaacg
     5461 ttccacctgt atcaatcgct gtgcgcaaag gtccgtgctc tcgtatcgtg gtaggacagc
     5521 atcgcggctg agaccagccg tactcctgca ctttaaatca gagaccgacc ttgaacggct
     5581 tctccgcctg gctcacagaa gcatccgccg aacagaagcg tactctgctg gccgcatcgc
     5641 tcggctggat gctcgacagc atggacgtga tgctctatgc tctcgtgctc ggccaggtcc
     5701 agcgcgaact ccatctgtcg gccgccatga gcggcgccat gatgtcgatg accctgctca
     5761 gcgctgccat cggcggaatc gccttcggct ggttcgccga tcgcgccggc cgcgtccgcg
     5821 ctctctccgc cagcatcctt atttactccg tcgccacgtt cctttgcggc ctcacccaca
     5881 ccgccctggc tctgctcacc tgtcgcatca ttctcggcct ggggatgggc ggcgaatggg
     5941 cctccggcgc cgcgttggtg gcggaaacct ggccggccca ccatcgcggc aaagctctcg
     6001 ccctcatgca gagttcctgg gctgtcggtt acgcgctggg cgccgctctg gtcgcggtca
     6061 tcatgccgca ttttggatgg cgtgccgtct ttttcttcgg caccgctcca gctctcgcag
     6121 cgctctggat ccagtcccgc ctgcacgagc ctgaagcgtg gcggcaggag cacaccgccc
     6181 gcccccggtg gggccaactg ttccgcggac gcctcggttt cagcactctc gtctgcgcca
     6241 ccatgaacgc agcctcgctc ttcgcctggt ggggtctctt cacctgggtg ccgcgctttc
     6301 tctcaatgcc catctcccag ggcggccgcg gactcggcat cgtgatgacc tccgaatgga
     6361 ccatcgtgat gcagatcggg acgttcctcg gctacgtcag cttcggctat atcgccgatc
     6421 gcttcagcca caagtacacc tacatcgcct atctcctgat cgccgcagtc atggtcccga
     6481 tcttcgcctc ggtcacaagc ccctcctggc ttctcattgt cgggccgctg gtcgggtttt
     6541 tcggcaccgg ctatttcagc ggctttgcca tcctcaccag cgagctcttc ccgaccgtac
     6601 tccgtgcctc agccatgggc ttcgtctaca acatcggccg cgttctcagc gccgtttctc
     6661 cctggctcat cggccgggtt tccgagcgaa gcgggctcgg ttttgcactc actctcacct
     6721 ccgccggttt tctcctggcc gccctgattg cctcggctct gcgatctccc gcgccgtcaa
     6781 ttgccgtcac tgatccctga cccgcttcct ttcacggatt cccgcatccc tactctgtac
     6841 atcgcaacct gtaccctgaa gctggtcctt atgaagattg gaatcacctg ctatccaact
     6901 tacggcggca gcggcgtcgt cgccaccgaa ctcggcattg aactggccgc agccggccac
     6961 caggttcact tcatcgccta ctcgcagccc ttccgcctgc gcggccgcga gcaggacatc
     7021 gccttccatg aagttccggt ctccaattac cccctcttcg aataccctcc ctacgactta
     7081 gccctcgcca cgcgcatggc cgaggtcgca gcctactacg aactcgacct gctgcacgtc
     7141 cactacgcca ttcctcattc catctccgcc tatctcgcgc gccagatgct tctccggcaa
     7201 aatcgccggc ttcctttcat caccaccctg cacggaaccg atatcactct cgtcggcctc
     7261 gaccattcct acctgcccat cacccgtttc ggcatcgacg agagcgacgg cgtcaccgcc
     7321 atttcgaatt acctgcgaga gaagacgatc caggagtttc atcccgaaag gccgattcac
     7381 gtcatcccca actttgtgaa ctgcgacgtg tacatgcctc tcgatgaaga cgcgcgcagg
     7441 gccggtcgcg cccgttatgc cgagccggga gagaagattc tcgtccacct gtccaacttc
     7501 aggcctgtca aacgcaccgc cgatgtgatc cgcatcttct cgcgcgtggc gcgtgagctg
     7561 cccgcgcgcc tgctgatgat cggcgacggt cccgaccgct ctgccgcaga atggatcgcc
     7621 catcgcaaag gcattcagga tcgcgtccac ttcctcggca agcaaagcag cgttacagac
     7681 attctgccgt tggccgacct cctgctcatg cccagcgaac tggaatcctt tggcctcgtc
     7741 gcccttgagg ccatggcctg ccgcgtaccc accatcgcga cgcaggtcgg cggaatcccc
     7801 gagctcatcg aagatggcgt gacgggccgc ctcttccctg tcggcgatgt tgaaggaatg
     7861 gccggagccg ctgtcgaact tctcagcgac tccagccgtt acctcgcgat ggccgaatcg
     7921 gcccgccgta ccgcgaaatc ccgctactgc gcctcaaaga tcatccctct ctacgagagc
     7981 ttctaccagg agatcctcag cgggtccgcc acaagtggcg tttagtgaat catgttgatc
     8041 gtgtcctgcg agatggtgtc gaacgttgtg atcgctttcg aactcgcctg aaacgcctgt
     8101 tgcgccacaa tgaggttggc aaactgcgtt gcgatgtcca cgttggcgcc ctccagcgcc
     8161 ttttcctgca gcgttcccaa tcccccggtc cccgcaatgc ccacggttgc cggtccggaa
     8221 gcggtggtgg tcgcgtagtt attcccggcc tcgatggtca gcccctgcag gttcgccaca
     8281 ttggccagcg ccacctggcc gaccacctgc gtctttccat tgctgaacgt ggccgagacc
     8341 actccggagg aatccacgct gaacccctga tagcttccgc tcgcataccc atcctggatc
     8401 gtgcccgtca ctggattgct cgaggccgac gtccccaccg cactctgagt gatcagccct
     8461 gcgccgctgg cgccaaacag atcccaggtc aggctcaggt tcgacgctcc gtcggtcagt
     8521 ccgcccagct gaatgccgac cacattcgtc gacggcgcaa tcagattgcc gttggtatcg
     8581 aacgtcaacg atcccgtagc attccctgaa gccgttcccg ccgcgctttc cagaagattg
     8641 cctcccagcg aaatcccggg gccggagata ctgagcccgt tcccgttcac cgagaccacc
     8701 actccctgga tgttggcgtt gccgattgcc gccgtcagcg ccgtggcgta agagttcagc
     8761 gtctccccgg gcgcgatggg cggggcggtg atcgtagtac tgttgccacc tccattcgtt
     8821 cctgtgatcg tcaggctggt cgatggatcg atggtcgtgc ccgcgtcgat gttgtacgcg
     8881 taggtggtta tccctccctc caccaccgtg ccggtgatcg cgccgccggt aactgtcagc
     8941 gtgctgccgt tgaggctggc cgtcgctgtg ctgcccgcgt tggtcagagc cgtctgcaac
     9001 gccgtcagat aggtgctcag cgactccccg gaagttgccg ccggtgccgt caccgttgta
     9061 accccgtcat tgatcgtaaa actcgtcgag gggtccattg tcccccctct gtcaagggca
     9121 aacgtattct gcgcgatgct tccctccacg acattccctg tgatcgtgcc tcccgttatc
     9181 gtcaacgtac tgccgctgac gctggccgtc gccgtgctgc ctgcattcgt cagcgctgtc
     9241 tgcagcgccg tcagatacgt agccagtgac tccccgctgg tcgccgccgg cgcggtcacg
     9301 gtcgtggtgc cgtcattgat cgtaaagctg gtcgtgggat ccaccgtcgc tccgctgttg
     9361 agggtgaagc tgttccgggt cacaccccct tccacgacac tgcccgtgat cgtgccccct
     9421 gtaatcgtca gcgtattgcc ggtgaggctg gccgtggccg tgctgccccc gttcgtcaga
     9481 gccgtctgca gcgccgtcag atacgtgctc agcgactcac cgttggttgc cgccggcgcc
     9541 gtcaccgtcg tactgccgtc atcgatcgtg aagctggtcg tgggatccac cgtcgtgccc
     9601 gcatcgaagg gaatcgtcgt attaccaccg cttgccgagg tcgttacggt ccgggtggtc
     9661 gccgacactg tatcggcgag cgtccgcgtc gtcgccgaca ccgtatcggc gatcgttcgg
     9721 gtggtcgccg acgacgtatc ggtgagcggt ttcggatcga gcgagaagtt gtaactccag
     9781 gtgttcgttc ccgtcttggt gaagtacacc gtcgcctcat gcggattgcc cagcgaatcg
     9841 taaatcggca cttgtcccag caccgtcgta cccaccgcgt cggaggcgtt caggttggcc
     9901 gtcatggaca tgctcgtcgt tgctcgggcg ttttccacct ggccgaccgg caactggatc
     9961 ggcgccagct gcgcactcgt gttcaccgcg ccgttggccg ccggataacc catgacgttc
    10021 tgcccatcct gagtcaccag acaaccgccg ctgatggtga agttgccatc ccgcgtcagt
    10081 tctgtcgtgc cgttgttgtc ctggaccaca aagaacccgt tgccgttgat ataaagatcc
    10141 gaagcattgc cggtcgaatt gatcccgccc tgcgaaaagt ctgtcaccgt cgccgccacc
    10201 ctggtgcctg cacccacctg ttgcggatcg cctgagcccg tggttccaat ctgctggtag
    10261 aaaagatcgc tgaacgtgac gctctggctt ttgaaggcgg tcgtgttcat gttcgacagg
    10321 ttgttggcaa tggtgttcag ctgggttgag gaagccttca aaccggtcag cggaatcgaa
    10381 aatgaggaca tagcttctcc ttatcaggcc gcgcgtctta acgcgccggg ctggtggttg
    10441 tcgttggaat cgcgctggcg cgaccgtgca tcgccgcgga tatggcgtcg aatggattcc
    10501 ctgcgggcat gcctgttgca ctcgcgcccg gggtttctgt tgatcggcct agagcgctgg
    10561 ccactcgcac agccgcggcc gccgaactcc ctggatctcc cgcgctcagg ttcccgccgg
    10621 ccacggccgt tgccggtcgc tttgactcga tcccggtgcg attcgccgcg tcgccgctcg
    10681 gtgtcgtaac gtcggatgtg ccgcccccca ggtcctggtt aatttccacc agttgctgca
    10741 ggctgttcac ctgcaccagc tgatcgatga atttcgtcgg atccgagttc gccgtcggat
    10801 cctggttctt catctccgca atcagcaatt gcagaaaatc gttcgccgtc actgtagccg
    10861 tgttcgtcga ggacgaaccg ctgccggacg gcgatccggc gtcctgtgcc tgagccgcca
    10921 gcggcgaagc tgccccgatt gtgtctggaa atatctgcat cgcactcctc ccgttctggt
    10981 tctttcagac ccgcacgctg atgtaggaca tcccctcccg gtccatctcc accggagaag
    11041 gttgcgccgg ccgctcctgt tgcgggctcc gcacaggcgt tctgccgtca ttccctgccg
    11101 gatcgccgga agagcgtccc tgaccgcccg tggatgagga gaatctctcc gaagacagcg
    11161 cgtcgatccg cacatgttct cccgcaagaa attcgcggac tgccggcagt tgcgccgcga
    11221 tggcgtggtg ggcttccgct gtgccggatg ccagcgtcgc cgatacctgt cctgccgt
//
LOCUS       DASZYN010000176         8379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1228069, whole genome shotgun sequence.
ACCESSION   DASZYN010000176 DASZYN010000000
VERSION     DASZYN010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8379
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228069"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /gene="treZ"
                     /locus_tag="VHE33_04445"
     CDS             complement(<1..1125)
                     /gene="treZ"
                     /locus_tag="VHE33_04445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HVT96732.1"
                     /translation="MHRFRVWAPNVAKISVEVAGARHALRKRDRGWWETDVNAAQSGM
                     DYAYFLDDDDAALPDPRSLWQPQGVHGPSRILDPSAFKWTDNGWEAPPLASAIIYELH
                     VGTFTPEGTFHAAQAKLDALLDLGITHVELMPVNSFPGVRGWGYDGVDLFAPHEPYGG
                     PEALSAFVNACHAKGLAVILDVVYNHLGPAGNYLGKFGPYFTDAHHTPWGDAVNLEDR
                     GSYEVRRFFCDNALMWLRDYHFDGLRLDAVHAYMDRSAINFMEQLGAEVRTLEGQTGR
                     NYVVIAESDLNDPRLVTAREAGGYGLDAQWSDDFHHALVTALTGDRSGYYADFGSIAD
                     IAKALRDVFVVDGCYSPYRERTHGRPVLNLPASRFLGYAQN"
     gene            complement(1186..3003)
                     /locus_tag="VHE33_04450"
     CDS             complement(1186..3003)
                     /locus_tag="VHE33_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HVT96733.1"
                     /translation="MASKIEDYALIGDCETAALVNREGSIDWLCWPGFSSPACFASLL
                     GSQKNGYWQICPASEGWRCSRQYRAHTLILETTYENDDGSVRLIDFMPIREHNSDVVR
                     IVEGVRGTLDMRMALGLRFDYGRTVPWVTAIEDGIRAVAGPNVAVLHASVPVHGENLH
                     TAAEFSVSEGDQVWFTLTYGESFRPDPKPINAERALKDTERFWTHWTSQLQYEGDYFE
                     AVERSAITLKALTFRPTGGQVAAVTTSLPEVIGGSRNWDYRYCWLRDTTFTLLAMTTA
                     GYYDEACEWQDWLLRAVAGSPAEVQILYGLKGERQTPEWEVDWLPGYESSRPVRVGNA
                     AAKQLQLDIYGELLDTFYHALHGMDRHTEQDFRVLALLLDHLADIWQRPDEGIWETRG
                     GREQFTYSKMMAWVAFDRAILIAKKLHCKAPPQWAKLRDTIHLEICEKAFNKEKNTFV
                     QFYGGKHLDASLLLMPIVGFLPATDPRVRATIEAIERNLMTGGLVMRYDTVEAHDGLP
                     PGEGAFLACSFWMVSALKAIGRIDDARSLFHRLLGLRNDLGLLSEEYDVERKRLIGNF
                     PQAFSHIALINAAFDLERSEEPHHRAHHRRRHAEPVGRL"
     gene            complement(3018..3785)
                     /locus_tag="VHE33_04455"
     CDS             complement(3018..3785)
                     /locus_tag="VHE33_04455"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HVT96734.1"
                     /translation="MRLQGKVAIVTGSGSGIGQSIAKRLGSEGAKVVINYRGSEAGAD
                     ETRRTIEASGGEGVVVRGDVTKMDDLRKLVDTAWEKFGSADILVNNAGMEKRSDFWDT
                     PEEEYDSQMAVNLKGPFFLAQAFVRRLRAAKKPGRIINISSVHEDMAFPGFTAYCCSK
                     GGLRMMMRNLTVELGPLGITINNIAPGAIVTPINKALLEDKTKLNALLEKIPLGRLGN
                     TDDVAGMVAWLASDEASYVTGATFLIDGGLMRDYHEQ"
     gene            4030..4407
                     /locus_tag="VHE33_04460"
     CDS             4030..4407
                     /locus_tag="VHE33_04460"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HVT96735.1"
                     /translation="MAVFCRNCGKNLADDARFCSGCGAQMQPPVAPPPYYPTSSVTRL
                     VRPRMGRMVAGVCQGLANAYAWDVVWVRVITVLLTVFGGGSGLLAYIIFWIVMPEEPL
                     ALPPMPPMAPMPPPPPQYQPPSQ"
     gene            4581..5384
                     /locus_tag="VHE33_04465"
     CDS             4581..5384
                     /locus_tag="VHE33_04465"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVT96736.1"
                     /translation="MHYYSDGAKLFYTTLGGGPAVLLLHPTPVDHRFWMPVAEILAGK
                     YRLIVPDLRGHGQSELGEGAITVEKLAADAGRLLDELQLPKALFAGCSIGGYTLFEIW
                     RTMPARVEGFAFCCSKAQADTEQQRAKRSETIGKIQERGTADFIENNLQTLIGSAARR
                     RFPEKVAEAREMMQAVAAENIVAVQQGLAERPDSVGTARTMRIPCCIIAGGEDPGSTP
                     ADMRLLAEQIRNGGYGAEYNEISDAGHFAPFEQPELVARILRRFFDSVI"
     gene            5511..7049
                     /locus_tag="VHE33_04470"
     CDS             5511..7049
                     /locus_tag="VHE33_04470"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HVT96737.1"
                     /translation="MNDLSEFLPTAGSKSTRGWMRRLAAIPSTTSKRGITLVLAGLMI
                     PMALAPLSAFAQDAPPPPPDQQYDYDQGPPPPDDDAAPPQQWNELSPDQLNELVAPIA
                     LYPDSLVAQVLAASTYPTQVVEADRFVEAQGGAGPDQIAEMVNGQPWDPSVKALCAFP
                     SVLSNMDKNLDWTTQLGNAYYNQPQDVMSAVQAMRQEAYERGDLRSGAQLNVDYEPGN
                     IVIEPANPAVVYVPYYDPWAIYGFRPWYRWYAPPPPPGWRVGVRFGFGLGIVVGGGWG
                     HYGWGYGHWGFAWGSHPYVAYNHVTYVSRSVTVVNHGYYGRFDRRPEARHYNVQAAHA
                     AYAAGERNGFNRGVNRGESNGFNRGERNGYNNGFRNGYNRGNENNNRPNNNLARPPIN
                     QGRPQNNPNFNRPNTPDNNNRQNFNRPNSDLARPPQNNRNFDRPTMPNNNNNRPENRN
                     FNRPNSNMARPPINQGRPQEQPHNEGRPSGGGGRPQGGGGGERPHGNGGGDHGDRGGH
                     PHGH"
     gene            7192..8280
                     /locus_tag="VHE33_04475"
     CDS             7192..8280
                     /locus_tag="VHE33_04475"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVT96738.1"
                     /translation="MGTDQTRKDRRAFLKGTTAAAAAVFTGAALPHAAGAERLPAVPG
                     DLPNNPRTQDAMPTRNLGRTGYKVGIFSLGGQAALEKPNNAAAAVPIINRALDLGVNY
                     IDTSSIYGGPLRWSEQYVGEVMKTRRKEAFLATKTKERTREGSMRMIDKSLQLLQTDH
                     VDLWQLHDIGTMKDINAIFAPGGAMEALLEMQRQKVVRYLGVTGHYRPEALMEALHRH
                     DFDCILMALNAADPHHFTFQEQLLPLAVEKQMGIIGMKIPGRSRLLSSFVPPPVEQQK
                     HMWEGMVITTTPGTLTMKEAIGYTLSKPVSTVIIGCDSIAQLEENVAIAREFTPYNEQ
                     QLAALTAKVEPVSKPGLFFRFYDHDAAG"
BASE COUNT         1631 a         2578 c         2556 g         1614 t
ORIGIN      
        1 gttttgcgcg tagccgagga agcgtgatgc gggcagattg aggaccggac gcccatgggt
       61 gcgctcccga tagggcgagt agcatccgtc aacgacgaac acatcacgca acgccttcgc
      121 gatatccgcg atcgatccga aatccgcata gtacccggag cggtcgccgg taagcgcggt
      181 caccagagcg tgatggaaat cgtcgctcca ctgcgcatcg agaccgtagc cgcctgcttc
      241 gcgcgcggtc accaggcggg gatcgttcag gtcgctctcg gcgatgacga cgtaatttct
      301 gccggtctgg ccctcgagcg tgcgcacctc cgcgccgagt tgctccatga agttaattgc
      361 ggagcgatcc atataggcgt gtaccgcgtc cagccgcagg ccgtcgaagt gatagtcgcg
      421 caaccacatc agcgcgttgt cgcagaagaa gcgccgcacc tcgtagctgc cgcggtcttc
      481 gagattcacg gcgtcgcccc acggcgtgtg gtgggcgtcg gtgaagtacg gaccgaattt
      541 gcccagataa ttacccgctg gaccgaggtg gttgtagacc acatcgagga tcactgcgag
      601 ccctttcgcg tggcaggcgt tcacgaatgc actaagcgct tccggtccgc cgtagggttc
      661 atgcggtgca aagagatcca cgccgtcgta accccatccg cgcacgccag ggaaggaatt
      721 caccggcatc aactcgacat gtgtgatgcc gaggtcgagc agagcatcga gctttgcctg
      781 cgccgcgtgg aacgtaccct ccggtgtgaa ggttcccacg tgcagctcgt aaatgatcgc
      841 gcttgcgagc ggtggcgctt cccagccgtt gtccgtccac ttgaatgcag atgggtcgag
      901 gatgcgagaa ggtccatgca cgccctgggg ctgccagagc gagcgcggat cgggtagtgc
      961 tgcatcgtca tcgtcgagaa aatacgcata atccatgcct gactgcgcgg cattcacgtc
     1021 cgtttcccac cagccgcggt cgcgtttccg gagcgcgtgc cttgcgcccg cgacctcgac
     1081 actgattttg gcgacgttcg gcgcccacac acgaaagcga tgcatcggaa agagattctc
     1141 cttgaagaga gtctggtggg cgggctcggt acccgccggt gcgactcaga ggcgcccaac
     1201 cggctccgcg tggcgtcgac gatgatgcgc ccggtgatgc ggttcttcgg agcgctccag
     1261 atcgaaagct gcgttgatga gtgcgatgtg cgaaaaggcc tgcggaaagt tcccgatcag
     1321 ccgctttctc tcaacgtcgt actcctcgga gagcaggccc aggtcgttac gcaggccaag
     1381 cagccgatgg aaaagagagc gcgcgtcgtc aattcggccg atggctttca gtgcactcac
     1441 catccagaag ctgcaggcaa ggaatgcgcc ttcgccggga gggaggccat cgtgcgcctc
     1501 cactgtgtca tagcgcatga cgagaccgcc cgtcatcagg ttgcgctcga tggcttcgat
     1561 ggtggcgcgc acgcgggggt ccgtggcggg cagaaatccg acgattggca tcaagagcag
     1621 agaggcgtcg aggtgctttc cgccgtagaa ctgaacgaat gtgttttttt ccttgttgaa
     1681 cgccttttca cagatctcga gatggatcgt atcgcgcagc ttagcccatt gaggcggtgc
     1741 cttgcagtgc agtttcttcg cgatcaggat ggctcggtcg aatgccaccc atgccatcat
     1801 cttcgagtag gtaaactgct cgcggccgcc gcgcgtctcc cagatgcctt cgtcggggcg
     1861 ttgccagatg tcggccaggt ggtccagcag aagcgccaat acgcggaagt cctgctcggt
     1921 atggcgatcc atgccgtgca gcgcgtggta gaacgtgtcg agtagttcgc cgtaaatgtc
     1981 caactggagt tgtttagcgg cggcattgcc cacgcgcacg gggcgcgagc tttcatatcc
     2041 gggcagccag tccacctccc attcaggcgt ctggcgctcg cccttcaggc cgtagagaat
     2101 ctggacctcg gcgggactgc cagcaaccgc ccgcaagagc cagtcctgcc actcgcaggc
     2161 ctcgtcataa tagcctgcgg tagtcatagc cagcagcgtg aacgtggtat cacgcagcca
     2221 gcagtagcga taatcccagt tgcgggagcc gccgatgacc tccggaagag acgttgtaac
     2281 cgctgcgacc tgaccgccgg tgggccgaaa ggtgagcgct ttgagggtaa ttgccgagcg
     2341 ctcgaccgcc tcgaagtaat cgccttcgta ttgcagttgt gatgtccagt gggtccagaa
     2401 gcgctcggtg tctttgagcg cacgctccgc gttgatgggt ttaggatcgg gccgaaacga
     2461 ctcgccatag gtcagcgtga accagacctg atccccttcg ctgacggaga attccgccgc
     2521 cgtatgaagg ttctcgccgt gcaccggaac cgaagcgtgc agcacggcga cgttgggccc
     2581 agctacggct cggataccgt cttcgatcgc cgttacccat ggcaccgtgc gcccgtagtc
     2641 gaaacgcagg cccagggcca tgcgcatatc caaggttccg cgaacgccct cgacgatccg
     2701 caccacgtcg gagttgtgct cacggatcgg catgaagtcg atcaggcgca ctgagccgtc
     2761 gtcattctca tacgtagttt cgagaatcag cgtatgagcg cgatactgcc gcgagcatct
     2821 ccagccctcg ctcgcggggc agatctgcca gtagccattc ttctgcgatc ccagcagcga
     2881 ggcgaaacaa gccggtgagg aaaagccggg ccagcaaagc cagtcaatcg aaccttcccg
     2941 gttgaccaaa gcagcagtct cgcaatcgcc gatcagggca tagtcttcga tcttcgatgc
     3001 cataggggac gtgcttctta ttgttcgtga tagtcgcgca tcaggccgcc atcgattaag
     3061 aacgtggcac ccgtaacata agatgcttca tccgaggcaa gccaggctac cattcctgcc
     3121 acgtcgtccg tattgcccag tcggcccagc gggattttct ccagcagggc attgagtttc
     3181 gtcttgtctt cgagcaaagc cttattgatg ggcgtgacga tggctcccgg agcaatgttg
     3241 ttgatggtga tgcccagcgg tcccagttcg acggtcaggt tacgcatcat catgcgcagg
     3301 ccgcccttgc tgcagcagta ggcggtgaag ccgggaaagg ccatgtcttc gtgtacggaa
     3361 ctgatgttga tgattcggcc gggtttcttc gccgcgcgca gacgccgcac gaacgcctgg
     3421 gccaggaaga aaggcccctt gaggttgacc gccatctggc tgtcgtattc ttcctccggc
     3481 gtatcccaga aatcggaccg tttttccatg cccgcgttgt tgaccagaat gtcggcggag
     3541 ccgaatttct cccacgcggt gtccacgagc ttgcggaggt cgtccatctt tgtgacgtcg
     3601 ccccgcacca caaccccctc gccgccggaa gcctcaatgg tgcggcgggt ttcgtcggcg
     3661 ccggcttcgc tgccccgata gttgatgaca actttggcgc cctcactgcc gaggcgcttg
     3721 gcgatgcttt ggccgatccc ggagccgctt ccggtcacga tggccacttt cccctgcaga
     3781 cgcatggatt cctctcctct tttctggttg acaatgtgtt ggatgccgaa aaggcccgca
     3841 gccggtcctg gatgctgggg ttgaccgaca gggaacccgg gcgggctttc ccacgtattc
     3901 tccactgtac gctgaagacg tggcggtccg gtgtccacag gaatcgggaa gcgccaggct
     3961 gcggcgttat gctgaagttt cagagcggag tggcaggcgc cccggcgcag ctccgcagga
     4021 ggattggcca tggccgtgtt ttgcagaaat tgcggaaaga atctcgccga tgatgcccgg
     4081 ttctgctcgg gctgcggcgc gcagatgcag ccgcccgtcg cgccgccccc ctattatccg
     4141 acctcttcag tgacgcgcct ggtgcgcccg cgcatgggtc gcatggtcgc gggcgtttgc
     4201 cagggactgg ccaacgccta tgcgtgggat gtggtgtggg tacgcgtgat cacggtgctt
     4261 ctgaccgttt ttggcggggg atcaggcctg ctcgcttaca tcatcttctg gatcgtgatg
     4321 ccggaggaac cgctggcgct gcctcccatg cctccgatgg cgcccatgcc tccgcctccg
     4381 ccgcagtatc agccgccgag ccagtaggga acagcggtta gcggataaca gacagcggaa
     4441 agcgggtagc ggatagcgtt tttcaacctg ccactctttc tgcagcttcc tgtcgagtgc
     4501 gctgctgttg atcagccacg aagcgtgaac ctctgtcagc tagccgctat tggttcgctt
     4561 acggcgtatc ctcttagtcg atgcactact actccgacgg cgcaaaactc ttctacacca
     4621 cgctcggcgg cgggcccgcc gtcctgctgc tgcatcccac gcctgtcgat caccgtttct
     4681 ggatgcctgt tgccgaaatt ctcgccggca agtaccgcct catcgtcccg gacctgcggg
     4741 gtcacgggca gtcggagctg ggcgaggggg cgatcaccgt cgagaagctc gcggccgatg
     4801 cgggacgcct gctcgacgag ctgcaactcc cgaaggcgct ctttgcggga tgctcgatcg
     4861 gtggctacac cctgttcgaa atctggcgaa ccatgccagc gcgtgtcgaa ggcttcgcct
     4921 tctgctgctc gaaggctcag gccgacacgg aacagcagcg cgcaaaacgc tctgagacga
     4981 tcgggaaaat ccaggagcgc ggaaccgcgg acttcattga aaacaacctg cagacgctca
     5041 tcggttctgc tgcgcgccga cggttccccg agaaagtggc cgaggcacgc gagatgatgc
     5101 aggctgttgc cgccgagaat atcgtcgctg tacagcaggg cctggccgag cggcctgatt
     5161 ctgtcggtac tgcgcgcacg atgcgtattc cctgctgcat tatcgctggc ggggaagacc
     5221 ccggcagcac gcctgccgac atgcgactgc ttgccgagca gatacggaac ggcggctacg
     5281 gcgccgaata caacgagata tccgacgcgg gccactttgc accttttgaa cagccggaac
     5341 tggttgcccg tatcctccgt cgattctttg attccgtgat ataaagaccc gggttctcgc
     5401 ccagcgcgcg caacttaaat gcgcgcttcg gtttcccata ggctgagcgg gtccagttcc
     5461 cgggccgctc tggataagga aaggcccaca ggggctgaaa gggaccttcg atgaacgatc
     5521 tttccgagtt tctacccaca gccggttcaa agagcacacg cgggtggatg cgccggctcg
     5581 ctgcaattcc ctccacgact tcgaaacgcg gcatcacgct ggttctggcc gggctcatga
     5641 ttccgatggc gcttgctcct ttatccgcct tcgcgcagga tgcaccgcct ccgccgcccg
     5701 atcagcaata cgattacgac cagggaccgc cgccccccga tgacgatgca gctccgccgc
     5761 agcagtggaa tgagctgagt cccgatcagc tgaacgagct ggtcgcaccg atcgccctct
     5821 atcccgactc actggttgct caggtgctcg cggcgtcgac ctatccgact caggtggtcg
     5881 aggctgaccg gtttgtggaa gcgcagggtg gcgcgggtcc ggatcagatc gcggagatgg
     5941 tgaacgggca gccatgggat cccagcgtga aagcgctgtg cgcgtttccg tcggttctct
     6001 ccaatatgga taagaatctc gactggacca cgcagttggg caatgcctac tacaaccagc
     6061 cacaggatgt gatgagcgcc gttcaggcga tgcgccagga ggcttacgag cgcggcgatc
     6121 tccgctccgg cgcgcagctg aatgtcgact atgagcccgg aaacatcgtc atcgaacccg
     6181 cgaatcctgc cgtggtgtac gtgccctact acgatccgtg ggcgatctac ggcttccgtc
     6241 cctggtaccg ctggtatgcg cctccgccgc ctcctggatg gagggtcggt gttcgctttg
     6301 gtttcggact tggcatcgtg gttggcggcg gctggggaca ctatggatgg ggctatggac
     6361 actggggttt cgcgtggggt tcgcatccct acgttgcgta taaccacgtg acctatgtct
     6421 cacgcagcgt aacggtcgtc aaccacggct actatggccg gttcgaccgg cgtccggaag
     6481 cccggcacta caacgtgcag gcagcgcatg cagcctatgc ggccggcgaa cggaatgggt
     6541 tcaatcgggg cgtcaatcgc ggcgaatcaa acggcttcaa ccgcggtgag cgtaacggct
     6601 ataacaacgg tttcagaaac ggttacaacc gcggcaacga gaacaacaac cgtccgaaca
     6661 ataatctagc gcggccgccg atcaaccagg ggcgtccgca gaacaacccg aatttcaatc
     6721 ggccgaatac gccggacaat aataaccggc agaacttcaa ccgtccgaac agcgatctgg
     6781 cgcggccgcc gcagaacaac cgtaacttcg accggccgac gatgccgaac aacaataaca
     6841 accgtccgga gaaccgcaac ttcaatcggc cgaacagcaa tatggcacgg ccgccgatca
     6901 atcagggtcg tccgcaggaa cagccgcata acgaaggccg gccttccggc ggcggcggtc
     6961 gtccccaggg aggcggtggt ggagagcgtc ctcatgggaa cggtggcggc gaccatggcg
     7021 accgaggcgg ccacccgcac ggacactagt catacgagga gcaaagctga agagggatgg
     7081 caaacctgcc atcccttttt tttgtgggtt caactgaagc ttcatttcct gcttcctgct
     7141 tctgcttcct gctggtaaaa cggtttgttg cctgcgaagg agagactcgc catgggaacg
     7201 gaccaaactc gcaaggatcg tcgcgctttt ctaaagggaa ccaccgctgc agcagcagcc
     7261 gtattcaccg gagccgctct gccgcatgct gccggggctg agaggctgcc ggctgtgccc
     7321 ggcgatctgc cgaacaaccc gcgcacccag gacgcgatgc ctacccgcaa cctcggtcgc
     7381 acaggctaca aggtcggtat cttctccctg gggggccagg ccgcgctcga gaagccgaac
     7441 aacgctgccg ccgccgtgcc catcatcaac cgagccctcg atcttggcgt caactacatt
     7501 gacacgtcct cgatttacgg cggccctctg cgctggagcg agcagtacgt cggcgaagtg
     7561 atgaagacgc gccggaagga agccttcctc gccactaaaa ccaaggagcg cacgcgggaa
     7621 gggtccatgc gcatgatcga caagtcgctg cagcttctgc agaccgacca cgtcgatctt
     7681 tggcagctgc acgacatcgg cacgatgaag gacatcaacg cgatctttgc gcccggcggc
     7741 gccatggaag ctctgctcga aatgcagcgg cagaaagttg tgcgctacct cggcgtcacc
     7801 ggccattatc ggcctgaggc actcatggaa gcgctccacc ggcacgattt tgactgcatc
     7861 ctgatggccc tgaacgccgc cgacccgcac cacttcacct tccaggagca gttgcttccg
     7921 cttgccgtcg aaaaacagat gggcatcatc ggcatgaaga tccccggccg cagccgtttg
     7981 ctctccagct tcgtgccgcc tccggtcgag cagcagaaac atatgtggga aggcatggtg
     8041 atcacgacga cgcccggcac gctcacgatg aaggaagcca tcggttatac actttcgaag
     8101 ccggtcagca cggtgatcat cggctgcgac tcgatcgcgc aacttgagga gaacgtcgcc
     8161 atcgcccgcg agttcacgcc gtacaacgag caacaactcg ccgcattgac cgcgaaagtc
     8221 gagccggtat ccaagcccgg actcttcttc cgtttctacg atcatgatgc tgcgggatga
     8281 gcagggaatc aggtgcggct gagagcgctg gaaagaagag gctctattcg aagaccgcgg
     8341 tactccagta gaaaagcatc gccagtgcgc agaaggccg
//
LOCUS       DASZYN010000177         2029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_457267, whole genome shotgun sequence.
ACCESSION   DASZYN010000177 DASZYN010000000
VERSION     DASZYN010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457267"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VHE33_04480"
     CDS             complement(<1..536)
                     /locus_tag="VHE33_04480"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HVT96739.1"
                     /translation="MGKSRILPRKFYLDPPDVVARGLLGKLLVREYETGAGSRKKTER
                     LAGRIVEVEAYFGLDDPAAHAFAGKTERNAVLFGPPGFAYVYFIYGMHFCLNFSCEPE
                     GHGAGVLLRAVEPVEGLETMARLRGLAPGGNPKLLTSGPGRLCEALAIDRETHNGVDV
                     TSARSGLHVEDDGFAPEK"
     gene            complement(537..1004)
                     /gene="bcp"
                     /locus_tag="VHE33_04485"
     CDS             complement(537..1004)
                     /gene="bcp"
                     /locus_tag="VHE33_04485"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HVT96740.1"
                     /translation="MEINDKVADFALQDDSEKTVHLKDFAGKPVVLFFYPKADTPGCT
                     IEACGFRDQFKKIQATGAVVLGISRDTPKAQAKFREKYDLPYSLLADVDEKVCNQFGV
                     LKEKNMYGKKVMGIERTTFLIGPDQRLLKVFPKVKPEGHAEEVLAVLKGLPSK"
     gene            1136..>2029
                     /locus_tag="VHE33_04490"
     CDS             1136..>2029
                     /locus_tag="VHE33_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96741.1"
                     /translation="MCAVPAFAQYVGRVDDQKQSGTHLRSTAILEYTGNDLKDMKQSR
                     LVPIAIWDGAQYQPGGLYLAQPAPLAVLSGTQYELRDSGKPAGFFNIRDAENLAGLWI
                     GVGAYEPLPPPKPRAAPSRSNHVYAVKDYDPDKPHFAHRPADEQQNQSTVPVPAAPSP
                     SPKAPPVDPDRPTLHPRSDSDSGSTANSSASPSEAHPSDSDDDRPTFHRRTNASAQAA
                     APQPAVDPDRPRLEYKQPTEQEKIDKAEALFGLPKEMNQIAGISDSRNSADTISWEFS
                     WANPEDEAKMKAALEQIAQKAI"
BASE COUNT          413 a          754 c          521 g          341 t
ORIGIN      
        1 atcttctccg gcgcgaatcc gtcgtcttcc acatgcaggc ccgatcgcgc gctggtcaca
       61 tcaacaccgt tgtgcgtctc gcggtcgatg gcgagggcct cgcagagacg gcccggcccg
      121 gaggtcagca actttgggtt cccgccaggg gcgagcccgc gcagccgcgc catcgtctcc
      181 agcccctcca ccggctcgac cgcgcgcagc agcacgccgg cgccgtggcc ctccggctcg
      241 caggaaaaat tcaggcagaa gtgcatcccg tagatgaagt agacgtaagc gaagcccggc
      301 ggcccaaaca acacagcatt gcgctccgtc tttccggcaa aagcgtgcgc ggccggatca
      361 tccaggccga aatacgcctc cacctcgaca atgcgccccg cgagccgttc cgtctttttg
      421 cgcgatcccg cccctgtctc gtactcccgc accagcagct tgccgagcag cccccgcgcg
      481 accacatccg gcggatcgag gtaaaacttc cggggcagga tgcgggattt ccccatctat
      541 ttcgaaggca gccccttcag caccgccagc acctcttccg cgtgcccctc cggcttcacc
      601 ttcgggaaca ccttcagcag ccgctgatcc ggcccgatca ggaatgtcgt ccgctcgatc
      661 cccatcactt tcttcccata catattcttt tccttcagca caccaaactg attgcaaacc
      721 ttttcgtcta catcagcaag gagggaatac ggaaggtcgt acttctcgcg gaacttcgcc
      781 tgcgccttcg gcgtgtcgcg cgagatgccg agcaccacgg cgccggtcgc ctgaatcttt
      841 ttgaactggt cgcgaaaacc gcacgcctcg atcgtgcacc ccggcgtatc ggccttggga
      901 taaaagaaca ggaccaccgg cttccccgcg aaatccttca gatgaaccgt tttctccgaa
      961 tcgtcctgaa gcgcgaaatc ggcaactttg tcgttgattt ccatttccgt cccttccccc
     1021 gcagattaag acgcgcgcaa ccacgcacga ttaagattaa ttctaagcag cgaggattat
     1081 ggtaagggcc cacacaacac ttcggatttg gccggcggca gcggcgctcg ctctcgtgtg
     1141 cgcggttcca gccttcgcgc agtacgtcgg tcgcgtcgac gaccagaagc aatccggcac
     1201 ccaccttcgc tccactgcga tcctcgaata caccggaaac gatttgaagg acatgaagca
     1261 gagccgcctc gtgcccatcg ccatctggga cggcgcgcag tatcagcccg gcggcctcta
     1321 cctcgcccag cctgcgcccc tggctgtgct ctccggcacg cagtacgaac tccgggactc
     1381 cggaaaaccg gcaggcttct tcaacatccg cgatgccgaa aatctcgccg gtctctggat
     1441 cggcgtcggg gcgtacgagc ctctgccccc gcccaagcca agagccgctc catcgcgcag
     1501 caaccacgtc tacgccgtga aggactacga ccccgacaag ccccactttg cgcatcggcc
     1561 cgccgacgag cagcagaacc agagcaccgt ccccgtaccg gccgccccca gtccatcccc
     1621 aaaagccccg cccgtcgatc ccgaccggcc cacgctccac ccacgctccg acagcgattc
     1681 cggctctacc gccaattcct ccgccagtcc ttccgaagcg cacccgtcgg actccgatga
     1741 cgaccgcccg accttccatc gccgcacgaa cgcatccgcc caggcggcgg cgccccagcc
     1801 tgccgtcgat cccgatcgcc ctcgcctcga atacaaacag ccaacggagc aagagaagat
     1861 cgacaaagcc gaagcgctct tcggattgcc caaagagatg aaccaaatcg ccggcatctc
     1921 cgacagccgc aacagtgccg acaccatctc ctgggagttt tcctgggcca accctgaaga
     1981 cgaagccaaa atgaaagcgg cgctggaaca aatcgcgcag aaggccatc
//
LOCUS       DASZYN010000178         3603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_647538, whole genome shotgun sequence.
ACCESSION   DASZYN010000178 DASZYN010000000
VERSION     DASZYN010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647538"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1598
                     /gene="der"
                     /locus_tag="VHE33_04495"
     CDS             <1..1598
                     /gene="der"
                     /locus_tag="VHE33_04495"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HVT96742.1"
                     /translation="SRADGRNRPRAARGSNIAARLAPSSRNERGPIQREGFTAAPGEA
                     REPREPFISLSARSPKATPPGRPVLAIVGRPNVGKSTLFNRLTGSRRSIVGDEPGITR
                     DRIYGEVNWNGRTARIIDTGGIIPDDEAQIPAEIFRQARFAFDEADAIVMVVDGRTEL
                     AAPDIDLARLLIKTGKPLFLAVNKIDAQSMEAAAENFRRLGIRNLVPISAEHGHGIGD
                     LLDEVFEVLPPAQEAEETDAPEADSPAELPRTHAEFDQHETKVAIIGRPNVGKSTLLN
                     ALTGSTRAIVSPIAGTTRDAVDEVIRYKDQDLRIVDTAGIRRKGKTHLMAEKLSVVMA
                     RRHLEAADVALLVIDATEGVTASDATIGGYAHESGRSVVIVVNKWDLVTSKRTDGKPP
                     ADPAIFERQLRSVLKYLSYAPVLFISASEGRNIARVLDTALRVAAERRKRVTTGQINR
                     FLDRVDFQRATVPMSKRIRIYYMTQAAVAPPTFVLFTDRDVKLHFAFERFLENQIRAS
                     FGFEGTPIWFKVRPRNERAAAKK"
     gene            2473..3498
                     /locus_tag="VHE33_04500"
     CDS             2473..3498
                     /locus_tag="VHE33_04500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVT96743.1"
                     /translation="MQALPVQTLTVAAVPVPQSDEYVATVKSRRSATLNPQVSGNLVR
                     ISVHSGEHVKSGQVMMEIDPSKQAAAVASQTATEQQKLALYQYNQSQVERQRKLFAAG
                     IISKDAVDQAEQAYANSKADYEAALATRHEQEQQLGYYKITAPFDGIVGDIPVHVGDY
                     VTTSTMLTTLDENKDLEAYIYIPTERAAQVHNGLAVQIVDNNGKPIENSSIDFVSPQV
                     DDQIQGILVKAPVKSPLLRTEQLVKARVIWGMSPKPVVPVLAVTRLGGQAFVFLAQEA
                     APGKFVAHEQAVDLGDTVGNNYAVLAGLNNGDKVIVSGTQFLQNNMPVMPLPPGPPPA
                     GQPHAGS"
BASE COUNT          734 a         1262 c          953 g          654 t
ORIGIN      
        1 cctctcgcgc cgacggccgc aaccggcccc gcgccgcccg tggttccaac attgccgctc
       61 gtctcgcccc atcgtctcgt aacgaacgcg gcccgatcca gcgcgaagga ttcacggcag
      121 cgcccggcga agcccgcgaa cctcgcgaac ctttcatttc cctgagtgct cgttcgccga
      181 aggcgactcc gcctggacgg ccagtcctcg cgatcgtggg ccgtcccaac gttggcaaat
      241 ccaccctctt caaccgcctc actggctcgc gccgttctat cgtgggcgac gagcccggca
      301 ttacgcgcga ccggatctac ggcgaggtca actggaacgg ccgcaccgcg cgcatcatcg
      361 ataccggcgg tattatcccc gatgacgagg cgcagatccc cgccgaaatc ttccgccagg
      421 cccgcttcgc attcgacgag gccgacgcca tcgtcatggt tgtcgacggc cgcaccgaac
      481 tcgccgcccc tgacatcgac ctcgcgcgtc tgctcataaa aaccggcaag cctctcttcc
      541 ttgctgtgaa caaaatcgat gcccagtcca tggaagctgc cgccgagaac ttccgccgtc
      601 tcggcatccg caacctcgtc ccgatctccg ccgaacatgg acacggcatc ggcgacctgc
      661 tcgacgaagt cttcgaggtc ttaccccccg cgcaagaagc cgaggagaca gacgcacccg
      721 aagccgattc gccagccgag cttccgcgta ctcatgcgga gttcgatcag catgagacaa
      781 aagtagccat catcggccgc cccaacgtcg gcaaatccac cctcctgaac gccctcacag
      841 gatccacgcg cgccatcgtc tcccccatcg ccggaaccac gcgcgacgcg gtcgacgaag
      901 tcatccgcta caaagaccag gaccttcgca tcgtcgacac ggccggcatc cgacgcaagg
      961 gcaaaacgca cctcatggcc gagaagctct ccgtggtcat ggcgcgccgt catctggaag
     1021 cagccgatgt cgccctcctc gtcatcgacg ctaccgaggg cgtcaccgca tcggatgcca
     1081 cgatcggggg ctacgcccat gagagcggcc gctccgtggt tatcgttgtc aacaagtggg
     1141 acctcgtcac ttccaaacgc accgacggca aaccgcccgc agaccctgcg atcttcgagc
     1201 gacaattgcg ctctgtcctt aagtacctca gctacgcgcc ggtgctcttc atctccgcct
     1261 ctgaaggccg caatatcgca cgcgttctgg ataccgcgct tcgtgtagct gccgaacgcc
     1321 ggaaacgcgt cactaccgga cagatcaatc gcttcctcga tcgggtcgac ttccagcgcg
     1381 ccaccgtccc tatgtcgaag cgcatccgca tctactacat gacccaggcc gccgtcgctc
     1441 cgcccacgtt cgtgctgttc accgatcgcg acgtgaaact gcactttgcc ttcgaacgct
     1501 ttctcgagaa ccagattcgt gcttcgtttg gatttgaagg cacgcccatc tggtttaaag
     1561 tccgcccgcg caatgaacgt gccgccgcaa aaaagtagca tgcagacgcg gcaactacag
     1621 taactacgtg aaaaattctt ttgcattcca cacccagttg cccgaaaaac tggcaacgcc
     1681 atcccgatgc ctgccgtcga aagagttatg aggcaataac gcttcaaccc cgaagaagga
     1741 ggatggatgt cccgtacacg gacgaaatca actgaatcgg caagcaagcg caatgtcttg
     1801 acggacctgc gggcccggag gggcaaagtg actcgagccg agtccccgac catggggccg
     1861 ctgctggaat acactggctc ttccggaggc acgacgaaat ggtgcggcct ctactttgga
     1921 ggcgacgcca gggcgggctc ctaggcttcc ccggcagcgg gaagcgtctc cgaccggtta
     1981 ctccagcgag cagtccctcc aaagattgcc ctgccacacc ggaccaggcg catagccgtg
     2041 gaagcagcag aaaagcagga agtgaaatgc ctccctgccg acacaatcct gacccggggg
     2101 tagctgaaac ccgagccaat cggacgggct gttcggaaac gaacagcccg tttcacgtct
     2161 ggggcgaatt ttgccttgcc accgcgtgtt ccccggtatg gcctcctctg aattttctga
     2221 agctcctgcc catagtctcg aatcgaaccc tgcaagctat ccagtcaaaa gagtccgcgg
     2281 tcacagaaaa cactcggcaa ttcctgtatc ctgaggtgtt tctgaatcgt ttgatttcgg
     2341 tcctgttcga ccctggttcg aggtcccgca gtgaaaaggt actccgccgt ttttctcctt
     2401 acgttgcccc tcctggccgc cctttccctt ggctgtaaca aggctcagcc tcagccacag
     2461 gctggtgcgg gcatgcaggc gcttccggtt cagaccctca ccgtcgccgc ggtgcccgtt
     2521 ccacagtccg acgagtatgt cgccaccgtc aagtcgcggc gctccgccac cctcaaccct
     2581 caggtcagtg gcaatctggt caggatctcc gtgcactccg gcgagcatgt gaaatccggg
     2641 caggtcatga tggaaatcga tccgtccaag caggcagccg ccgtcgcctc tcagaccgcc
     2701 accgagcagc agaagctcgc gctctaccag tacaaccaga gccaggtcga gcgccagcgc
     2761 aaactatttg ccgccggcat catcagcaag gacgcggtgg atcaggccga acaggcctac
     2821 gcgaattcca aagccgacta cgaagccgct ctcgccaccc gccatgagca ggagcaacag
     2881 ctcggctact acaagatcac cgcgccgttc gatgggatcg tgggcgacat cccggtccac
     2941 gtcggcgatt acgttacgac ctccaccatg ctgaccacgc tcgatgagaa taaggacctc
     3001 gaggcctaca tttatatccc caccgagcgc gctgctcagg ttcacaatgg cctcgcagtt
     3061 cagatcgtcg ataacaacgg caagcccatc gagaattcct ccatcgactt cgtatcacca
     3121 caggtcgacg accagatcca gggaattctg gtcaaggccc ccgtcaaatc tcctctgctg
     3181 cgcaccgagc agctcgtcaa ggcgcgggtc atctggggca tgtcgccgaa gccggtcgtt
     3241 cccgtgcttg ccgtcacccg cctcggcggc caggcctttg tctttctggc ccaggaagcc
     3301 gctccgggca agttcgtcgc ccacgaacag gcggtcgatc ttggcgatac ggtcggcaac
     3361 aattacgccg tgctcgccgg cctgaacaac ggcgacaagg tcatcgtctc cggcacccaa
     3421 ttcctgcaga acaacatgcc cgtgatgccg ctgccccccg gccccccgcc tgcaggtcag
     3481 ccccacgccg gcagctagcc cgcatgatcc gaacagagag gtacggagag gctacccttg
     3541 gttgatttct ttattcaccg cccggtcttc gccactgttt gcgcgctcct catcatcctc
     3601 gcg
//
LOCUS       DASZYN010000179         7073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_647542, whole genome shotgun sequence.
ACCESSION   DASZYN010000179 DASZYN010000000
VERSION     DASZYN010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647542"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VHE33_04505"
     CDS             complement(<1..407)
                     /locus_tag="VHE33_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96744.1"
                     /translation="MMLAMIEAAGRALLFALVVGAVLRLFRVSNVPVRKAAWTLVLIA
                     SLAMPFLMRSPAVASWNAQWSWAVPVPARWLAPDTALEPVQPAPVHAAPVALGARARD
                     PQAMPASATQIKAAPVAVAVSLDVTPVPAAAPIA"
     gene            complement(404..811)
                     /locus_tag="VHE33_04510"
     CDS             complement(404..811)
                     /locus_tag="VHE33_04510"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HVT96745.1"
                     /translation="MARNTASEHDPAELGELERGILAIVWRLGEVTAEQVREEVERPL
                     KDSTIRTVLRRLEEKGYLAHAVDNRTFIYRPAESPQKVAARAVQRIVDWFCQGSVEEL
                     LVGLLDSKVVSRAELEKLSKRVAAAKKQEGGKG"
     gene            complement(985..2229)
                     /locus_tag="VHE33_04515"
     CDS             complement(985..2229)
                     /locus_tag="VHE33_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HVT96746.1"
                     /translation="MNAEIIAVGSEMLTPFRQDTNSLYLTEKLNELGVSVDFKTIAGD
                     RLKQLADVVRHALGRVDLVVVSGGLGPTEDDLTREAVAEALGVKVKRDPDLIAHLYAR
                     AASRRMTITRNNEKQADVIEGATMLPNKLGSAPGQWIDTVVGKHRKLAMLLPGPPHEL
                     KGMFEDQCLPRLREIVPKRHIATRVLKAAMIGESAADARIAPIYTQYKDVDTTILAHL
                     GDIELRLSCSKPIAELAQTRVDELAGKLEDELDEFVYSSQGETLEQIVLYHLEMRGAS
                     LAVAESCTGGMVAERLTRVSGSSRSFVGGAVVYSNRLKTEFAGVDPQLIAQHGAVSQE
                     VALALAEGIRERCGATIGLGITGIAGPGGGTEEKPVGLVYVALSDADGTEWVEKRFSG
                     DRAAIRFYASQQGLDMVRRRLM"
     gene            complement(2273..3340)
                     /locus_tag="VHE33_04520"
     CDS             complement(2273..3340)
                     /locus_tag="VHE33_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96747.1"
                     /translation="MKSEPSTAPRIDTISPSAALPGGDVEVQGANLGGVAVSLGAANT
                     IGGGEWRRPIAMLGDLAAPVLLSRNARLTLRVPDDAESGRLRILQNGAQSNAVEVRVA
                     SLIATGVHAVANPVMDHSGNIFVTFSGQRGQDSPVSVFRIERDGEMRPFVRGIVNATG
                     LAVDYEENLYVSSRHEGKVYRVAKNGASAVYAEGMGVASGLAFDADQNLYVGDRSGTI
                     FKIAPDRQIFVFATLEPSVAAYHLAFGPDGTLYVTGPTTSSYDAVYAISRDGETNVLH
                     RGLGRPQGVAVDIAGNVYVAASLQGRRGVAMITQAGEASLVVSGPGIVGFNFVPGGGA
                     LVATNTSVFHLALGIEGWRLF"
     gene            complement(3387..3926)
                     /locus_tag="VHE33_04525"
     CDS             complement(3387..3926)
                     /locus_tag="VHE33_04525"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HVT96748.1"
                     /translation="MTRKTELPPPGVRIYEGGTPAPPARTSWAWNAGTVFGIGRLKPG
                     PGTWASLAAAILWYVGLSAAHLTDWAAVLVTLSAAAVVTLIGIRASTIVERESGRTDP
                     GFVVIDEVAGQWVTLAFTPLDIGHLLVGFALFRFFDIVKPWPVRRVERLSGGSGIMLD
                     DVAAGLYGLAVMALLHLWW"
     gene            complement(3910..4125)
                     /gene="yacG"
                     /locus_tag="VHE33_04530"
     CDS             complement(3910..4125)
                     /gene="yacG"
                     /locus_tag="VHE33_04530"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HVT96749.1"
                     /translation="MTTKILRCPTCRTIVTQQEENFPFCSDRCRVIDLGKWASGAYRI
                     SSPVLDPEVLEGLGDLPQPHGEHDEED"
     gene            complement(4122..5726)
                     /gene="rimO"
                     /locus_tag="VHE33_04535"
     CDS             complement(4122..5726)
                     /gene="rimO"
                     /locus_tag="VHE33_04535"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HVT96750.1"
                     /translation="MLTEEAPAVRESRPRVGFVSLGCPKNLVDSEVMMGLLDRAGAEL
                     TPHAETADILVVNTCSFIDQAKQESVDAILEMAKLKTTGSAQRLVVAGCLVERYRDEI
                     QRNIPEVDAVVGTGELDQILSAAGLHVAPPAPVSSSPFTILAHGPAEVRPGRETLAGS
                     HKDAGSPVFRPEGDRREAQGRFSRNDWDGASPQLPQYLYDHTTPRLRTTKSASAYIKI
                     AEGCDHPCSFCVIPNLRGKFRSRRFESVVAEAEALVAEGVREITLIGQDTTCYGEDLG
                     LRDGLAELLDRLAGIEGLIWLRFLYAYPNRITGRLLETIAKHDNICKYLDVPLQHASA
                     SVLKTMKRGAGAEIFLKMLAKIRAEVPGVALRTSFIVGFPGETDADFEALCDFVADAK
                     FDWLGVFAYSDEEGSKAFDLDGKVPKRVIEQRRRALMKLQKGISRQAMRGRIGQIVDV
                     LAEGESAETPLLWEGRTQSHAPEIDGKVYLNDFGPLENLTPGRFYRCEITEAHDYDLV
                     ARVTAESHRRDVRREERELAGAGVEL"
     gene            complement(5783..6271)
                     /locus_tag="VHE33_04540"
     CDS             complement(5783..6271)
                     /locus_tag="VHE33_04540"
                     /inference="COORDINATES: protein motif:HMM:NF021453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2165 domain-containing protein"
                     /protein_id="HVT96751.1"
                     /translation="MIVRLSKTVLVAAVAFFNTLVVFNNLTDYGTNYTFVHHVLWMDS
                     TFPGNHGMWRAIHPLWIHAAFYDSIILWEAVSALLLWAGSVKLLRASGKPGAAFREAK
                     SLAVGGLTVNLLMWVVAFLSVGGEWFLMWQSPTWNGQEEAFRMFLVVGLVLLYLAMPE
                     AQ"
     gene            complement(6268..>7073)
                     /locus_tag="VHE33_04545"
     CDS             complement(6268..>7073)
                     /locus_tag="VHE33_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96752.1"
                     /translation="TQRVQLLGQRSSPQDLQVSGELPGVPAGQTRFIRYVDLSRLAQV
                     SHRVKDDENFEHAVELTGVPLDVLISALGLSAERVLIAADASDNYEAHYTAEYRAQHH
                     PFLVLRIDGKEPAKWPKGPDGENYGPYLISHPSFTPAFHILAHKDMAQIPFAVVGLRF
                     LDEDAVLKELLPPVKAAAASPAMEGSRIAEQNCLRCHRDGDIGGTKSPFGWPQMALIA
                     QGNPAAFGKYVVRPNSVNPEANMPANPDYDATTVAALTAYFQSFAPGGK"
BASE COUNT         1420 a         2451 c         1899 g         1303 t
ORIGIN      
        1 gcaatcggtg cggcagcagg aaccggggtc acgtccagcg aaacggcgac agcaaccggg
       61 gcagccttga tttgcgtcgc agaagccggc attgcctggg gatcccgggc ccttgccccc
      121 agcgccaccg gcgcggcatg aacgggtgca ggctgaaccg gctccagcgc cgtgtcagga
      181 gccaaccagc gagccggcac cggcaccgcc caactccatt gagcgttcca actggcaacg
      241 gccgggctcc gcatcaggaa aggcatcgcc agcgaggcga tcagcacgag cgtccacgcc
      301 gccttacgca ccggcacatt gctgacgcgg aacaggcgca gcaccgcgcc cacgaccagc
      361 gcgaacaaca gcgctcgacc cgccgcttcg atcatcgcaa gcatcatcct ttgcctccct
      421 cctgcttttt ggccgccgcc acgcgcttcg aaagcttttc cagctcggct cggctgacca
      481 ccttcgagtc cagcagaccc accagcaact cctccaccga gccctggcag aaccagtcca
      541 cgatgcgctg caccgcacgg gccgcgacct tctgcggcga ctcggcgggc cgatagatga
      601 acgtccggtt atcgaccgcg tgtgccagat agcccttctc ctccagccgt cgcaacacgg
      661 tgcgaatcgt cgagtccttc agcggacgct ctacctcttc ccttacctgc tccgccgtca
      721 cttcgcctaa ccgccagaca atcgccagta tgccccgctc cagctctccc agttcggccg
      781 gatcgtgctc cgatgcagtg ttacgtgcca tagtgttacc cactgtaaca cataagactg
      841 ctcgattcgc aagagggtga tcagcgattt ttgcgttttg agaatcctcg cagctacggg
      901 ctgcaaccac taagaccgga tcggcagccc ccggttaggc acgaatttcg ccgtaggctg
      961 agggaaacga gaagaaagct gccactacat caggcgccgg cgcaccatgt ccagcccctg
     1021 ctggctggcg tagaagcgga tcgctgcgcg atctcctgaa aaccgtttct caacccactc
     1081 ggtcccatcg gcgtcactga gagccacata caccagcccc accggctttt cctcggtgcc
     1141 gccaccggga cctgcgatcc cggtaatacc gagtccgatc gtggcaccgc agcgctcccg
     1201 aatcccctcc gccagagcga gcgcaacctc ctggctcacg gcgccgtgct gcgcgatcaa
     1261 ctgcggatca acgcctgcaa actcagtctt cagccgattg ctgtagacca cagctccgcc
     1321 cacgaatgag cgcgaacttc cgctcaccct cgtgagccgt tcggccacca tgccgcccgt
     1381 gcagctctcg gcaaccgcca gactcgcgcc gcgcatttca aggtggtaga gcacaatctg
     1441 ctccagtgtt tcgccctgcg aggagtagac gaactcgtcc agttcatcct ccagcttgcc
     1501 cgccagctca tctacgcgcg tctgggccag ttcggcaatt ggttttgagc aggaaagccg
     1561 caactcgatg tcgcccagat gcgcgaggat ggtcgtgtcg acatccttat attgcgtgta
     1621 gatcggtgcg atcctggcgt ccgccgccga ttcgccaatc atggcagcct tcagaacccg
     1681 ggtcgcgata tgccgcttcg gcacgatctc ccgcaaccgc ggcaggcatt gatcttcgaa
     1741 catgcccttc agctcatgcg gcggaccggg caacagcatc gccagcttgc gatgcttccc
     1801 cactaccgta tcgatccatt gcccaggtgc cgatcccagc ttgttgggca gcatcgtcgc
     1861 gccttcgatc acgtccgcct gcttttcgtt gttccgggtg atcgtcatcc ggcgcgatgc
     1921 tgcgcgcgca taaagatgcg caatcaggtc cggatcgcgc ttcaccttga cgccgagcgc
     1981 ctcagccaca gcttcgcgcg tcaaatcatc ttctgtcggc cccaggcctc ccgatacaac
     2041 aacaaggtca acccgtccga gtgcgtgccg caccacatcg gctaattgct tcagccggtc
     2101 gccggcaatg gtcttgaaat cgaccgacac gcccagttcg ttgagtttct ctgtcagata
     2161 cagggaattg gtgtcctggc ggaacggcgt gagcatctcc gatccaactg cgataatctc
     2221 cgcgttcatc gatctccaga attccgatga tttacctggg cctgcgcggg catcagaaca
     2281 accgccagcc ctcaatcccc agcgcgaggt gaaaaaccga ggtatttgta gccaccagcg
     2341 ctccgccgcc gggtacaaaa ttgaagccca cgatgccggg gcccgagaca accagcgacg
     2401 cttcgccagc ctgcgtgatc atggcaacgc cgcgcctgcc ctgcagagac gccgccacgt
     2461 agacgtttcc cgcgatatcg accgccacgc cctggggccg ccccaggccg cggtgaagaa
     2521 cgttggtctc gccatcccgg ctgatcgcgt agaccgcatc gtagctggag gttgtcggtc
     2581 cggtgacata cagggtcccg tccggaccaa acgccaggtg ataggcggcc acgctcggct
     2641 ccagcgttgc aaagacaaag atctggcgat ccggcgcaat cttaaagatg gtcccgctgc
     2701 gatcgccgac atacagattc tgatccgcat cgaacgcgag cccactggcc acccccatgc
     2761 cctcggcata aacagcggag gctccattct tggctacgcg atacaccttg ccttcatgac
     2821 gcgacgagac atagagattc tcttcatagt caaccgccag accggtcgca ttcacaatgc
     2881 cgcgcacaaa gggccgcatc tcaccgtcgc gctcaatgcg aaataccgaa accggcgaat
     2941 cctggccccg ctggccggaa aacgtcacga agatatttcc cgaatgatcc atcaccggat
     3001 tcgcaaccgc gtgcacgccg gttgcgatca ggctcgcaac tcgcacctcc acagcgttac
     3061 tctgcgcacc attctgcaaa atgcgcagcc gcccggactc agcgtcatcg ggaacacgga
     3121 gcgtcagcct ggcgttgcga ctcaagagaa cgggtgccgc caggtccccg agcatggcga
     3181 tgggacgccg ccactccccg ccgccgatgg tatttgctgc gcccagcgaa accgcaacgc
     3241 cgcccagatt tgcgccttgc acctctacgt cgccgcccgg cagcgcggca gagggcgaga
     3301 tcgtgtcgat cctgggcgcc gtgctcggtt ccgatttcaa aattcgtacc attccctacc
     3361 ctgtctcgtt ctgccagccc tgaagatcac caccacaggt gcagcagcgc catcacagcc
     3421 aaaccgtaaa gaccggcagc gacatcatcc agcatgatgc cgctcccccc tgagagcctt
     3481 tccaccctcc gcaccggcca cggtttcacg atatcgaaga accggaacaa tgcgaaaccc
     3541 accaaaagat gccctatgtc caagggtgta aaggccagag tcacccactg ccccgccacc
     3601 tcgtcgatca cgacaaaacc cggatcggtg cgcccgctct cccgctcgac gatcgtgctg
     3661 gctcgaatcc cgattaacgt cacaaccgca gcagcggata aagtcaccag gactgctgcc
     3721 caatccgtaa gatgcgccgc ggaaagcccg acataccaaa ggatggccgc ggccagtgag
     3781 gcccatgtgc ctggaccagg cttgaggcgg ccgatcccga aaacggtgcc tgcattccac
     3841 gcccacgagg ttcttgccgg tggcgccggt gtgcctcctt cgtaaatccg gacgcccgga
     3901 ggtggcagtt cagtcttcct cgtcatgctc tccatgcggc tggggcagat cacccagtcc
     3961 ctccagcact tccggatcga gcacgggcga ggaaatgcga tacgctccac tggcccactt
     4021 gcccagatcg atcacgcggc atcggtcgct gcagaaagga aaattctcct cctgctgggt
     4081 cacgatggtg cggcaggtcg gacaccgcag gatctttgtc gtcacagttc cacccctgcc
     4141 cctgccagct cgcgttcctc cctccgaaca tccctcctat gagattcggc cgtcacgcgc
     4201 gccaccagat cgtagtcgtg cgcctctgtg atttcgcagc ggtaaaaccg tcccggagtc
     4261 aggttctcca acggaccgaa atcgttcagg tagaccttgc cgtcgatctc cggcgcgtgc
     4321 gactgcgtcc ttccttccca gagcagaggc gtctccgcag actcaccttc agccagcaca
     4381 tcgacaattt ggccgatgcg tccacgcatt gcctgccggc tgattccctt ctgcagcttc
     4441 atcagcgcac gacggcgctg ctcgatgacc cgcttcggaa ccttgccgtc cagatcgaat
     4501 gcctttgagc cttcctcgtc ggagtaagcg aagactccca gccagtcaaa ttttgcgtcg
     4561 gccacaaaat cgcacagggc ctcaaagtcg gcgtctgtct cacccggaaa tccgacaatg
     4621 aacgacgtgc gcagcgcgac tcccggtact tccgcgcgaa tcttcgcgag catcttcaga
     4681 aaaatctcgg ccccggcgcc acgtttcatc gtcttcagca cggaagcgga tgcatgctgc
     4741 agcggcacgt cgagatactt acagatgttg tcgtgcttcg cgattgtctc cagaagccgc
     4801 cctgtgatcc ggttcggata ggcgtagagg aaccgcagcc agatcagtcc ctcaatcccc
     4861 gccagccggt ccagcaactc cgccagcccg tctctcagcc ccagatcctc gccatagcag
     4921 gtggtgtcct ggccgatcag cgtaatctcc cggaccccct ctgcgaccag ggcttcggct
     4981 tcggcaacga ccgactcaaa tcgacgcgaa cgaaacttcc cccgcaggtt cggaatcacg
     5041 cagaagctgc aggggtgatc gcagccttca gcaatcttga tgtaagctga cgcgcttttc
     5101 gtggttcgca gccgcggtgt ggtgtggtcg tacaaatatt gcggcagttg cgggctggcg
     5161 ccatcccagt cgttccggga gaacctcccc tgcgcctcac gccgatcccc ctccgggcga
     5221 aaaaccggag atccggcgtc cttgtgcgaa cccgccagag tctcacgccc agggcgcact
     5281 tctgcaggac catgcgcaag aatcgtaaag ggcgaggaac tgaccggcgc aggcggtgca
     5341 acatgaaggc cggctgcact cagaatctga tccaactccc ccgtcccaac aacagcgtcc
     5401 acttccggga tgttcctctg aatctcgtcg cgatagcgtt caacgaggca cccggcgaca
     5461 accagccgct gcgccgaacc tgttgtcttc agcttcgcca tctcgagaat cgcgtccacc
     5521 gattcctgtt tcgcctgatc gatgaacgaa caggtattga ccaccaggat gtcagccgtc
     5581 tccgcgtgcg gcgtcagctc agcgccggct cgatccagca gccccatcat gacttcgctg
     5641 tcgaccagat tcttcggaca ccccagcgag acaaagccca ccctgggccg cgattcccgc
     5701 accgccgggg cctcttctgt cagcaattct tccttcacat ccctatttta gcgggtatgc
     5761 acagcctgcc tcggtcgcgg catcattggg cttccggcat cgccagatac aggagcacca
     5821 aaccgaccac caggaacatc cggaatgcct cctcctggcc gttccaggtg ggcgactgcc
     5881 acatcaggaa ccactcgccg ccgacgctca ggaatgcgac gacccacatg agcagattca
     5941 ccgtcagccc gccgacggcc agcgattttg cctcgcgaaa tgcggctccc ggcttcccgg
     6001 acgcacgcag cagcttcacg gatccggccc acagcagcag cgcgctgacg gcttcccaaa
     6061 ggatgatcga gtcgtaaaac gccgcatgaa tccaaagtgg atggatcgcg cgccacatgc
     6121 cgtggttgcc gggaaacgtc gaatccatcc acagcacgtg gtgcacgaag gtgtaattgg
     6181 tcccgtagtc ggtcaggttg ttaaagacca caagggtatt gaagaaggca accgccgcca
     6241 ccagtaccgt tttcgagagg cgtacgatca ctttcccccc ggagcaaagc tctgaaaata
     6301 ggccgtgaga gccgcaaccg tcgttgcatc gtagtccggg ttcgccggca tattcgcctc
     6361 cgggttcacc gaattcggcc gcaccacata cttgccgaac gcggctggat tgccctgagc
     6421 aatcagcgcc atctgcggcc agccgaaggg cgacttcgtc ccgccaatat cgccgtcccg
     6481 atgacaacgg aggcagttct gttccgcgat tcgcgagcct tccatcgccg gcgatgccgc
     6541 tgcagcctta accgggggca gcagttcctt cagcaccgcg tcctcgtcca gaaagcggag
     6601 tccaaccacg gcaaacggaa tctgcgccat gtccttgtgc gccagaatgt gaaatgccgg
     6661 tgtgaaagat ggatgcgaaa tcagataagg cccgtaattc tccccatccg gccccttcgg
     6721 ccacttcgcc ggttcttttc cgtcgatccg caacaccaga aaaggatgat gctgcgcccg
     6781 atattccgca gtgtagtgag cctcatagtt atcgctggca tccgctgcga tcagcactct
     6841 ttccgcgctg agtcccagcg cggaaatcag gacatcgagc ggaactccgg tgagttccac
     6901 cgcatgttcg aagttctcgt cgtctttgac tcgatggctc acctgggcca ggcggctcaa
     6961 atcgacgtag cgaataaagc gcgtttgccc cgccggaact cccggcaact ctcctgaaac
     7021 ctgcagatcc tgcggtgagc ttctctgtcc caggagttgc acgcgctggg tcg
//
LOCUS       DASZYN010000180         2793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_775052, whole genome shotgun sequence.
ACCESSION   DASZYN010000180 DASZYN010000000
VERSION     DASZYN010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775052"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2044
                     /locus_tag="VHE33_04550"
     CDS             <1..2044
                     /locus_tag="VHE33_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HVT96753.1"
                     /translation="FGKDPSVVGTSFFVNGNPFTIVGIGPPGYFGERLTTDPPSLWIP
                     LNDNPVVSGGAFDVLESPQLDWLNLIGRLEPGADKKKMEAQLQVELRQFLQDPVAKVE
                     ARSAGLIPKQTLHLSYGGGGVQQMQDDSRDELHLLMWISAFVLLIASANLANLMLVRA
                     TARKAQTSVRAALGAPRSRLVRQALTESVVLAVLGGAAGLAVAYGGARLLLHMVAGKS
                     YLPIDATPSLPVLGFAFGVSLLTGILFGTAPAWMTAHTDPIEALRGANRSTKHSGMWT
                     QKILVVMQAAVSLALLCAAGLLIRSLTNLQNQHFGFETKNRYILHIDPQMAGYQANQA
                     DTFFREMRDSLATIPGVKQVTYSLYSPMEGDNWGEGVYIEGQAPPPPGSNFNNASWVR
                     ASANYFDNIGTKIIAGRGITEQDTGSTRAVAVVNETFAKHFFKDGQALGKHFGDLDQK
                     YAGNFEIVGVTEDTQYWSPMSRIRPMFFLPGPQWQHYSDPAEVMFENVSHLEMNAVEI
                     QTAGYVPGLESQVRRVLSQINPNLTIISFETFAEQVKERFNDQEIMVQLTSVFGFLAL
                     ALAAIGLYGVTAYSVAQRTSEIGIRMALGADRGGILQMVLRSAFLQAGIGLAIGMPAA
                     IFGGRFMASMLYHVKTWDPVVLVVTTLILAVAAFLAAVIPAQRAASVEPMTALRVE"
     gene            2124..2522
                     /locus_tag="VHE33_04555"
     CDS             2124..2522
                     /locus_tag="VHE33_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96754.1"
                     /translation="MISESLHKWLGRVADREALCAWLSRLMLLLVAIELVTMPLTQQL
                     WTWDGFVRGEPDFELGLFMVVACLCLSLLRAQHSRQRISLLLVISRWLSEVLRERFKS
                     VLLWALLSAQTSPEHLFPRTPRRITTPLLI"
     gene            2608..>2793
                     /locus_tag="VHE33_04560"
     CDS             2608..>2793
                     /locus_tag="VHE33_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96755.1"
                     /translation="MKKSEVLSAWARILSGRAPSLSIEITKECPLRCPGCYAFDAAHL
                     GGATELRELSDFRGDELV"
BASE COUNT          534 a          739 c          895 g          625 t
ORIGIN      
        1 attcggcaag gatccgtccg tagtggggac atcgtttttc gtcaacggca accccttcac
       61 catcgttggg attggacctc ccggttattt tggcgagcgg ttgacgacag atcctccatc
      121 gttgtggatc ccgctcaacg acaatcccgt ggtttccgga ggcgccttcg atgtactgga
      181 aagcccccaa ttggattggc tgaatctgat cgggaggctg gaacccggcg cggacaaaaa
      241 gaaaatggag gcacagctgc aggttgaact acgccagttc ctgcaggacc cggttgcgaa
      301 ggtcgaggcg cggagcgcag gactgattcc caaacagact ctccatcttt cctatggcgg
      361 cggcggtgtg cagcagatgc aggacgactc ccgagatgaa ctccacctgc tcatgtggat
      421 ctcggctttt gtgctgctga tcgcttcggc gaatctggcc aacctgatgc tggtccgagc
      481 aacggcgcgg aaggcgcaga cttcagtgcg agccgcgctg ggagctcccc ggagccggct
      541 ggtgcggcag gctctgacgg agagtgtcgt gctggcggta ctgggcgggg cagcgggcct
      601 ggcggtcgcc tatggcggcg cgcggcttct gcttcacatg gtggcaggaa agagctatct
      661 gcccatcgac gccacgccgt cgttgccggt actgggattc gcgttcggcg tttcacttct
      721 gacgggcatc ctgtttggga ctgcgcccgc atggatgacc gcacacaccg atccgatcga
      781 agctctgcgc ggagccaatc gatcgacgaa gcacagtggt atgtggaccc agaagattct
      841 cgtggtgatg caggcggccg tttctctcgc ccttttgtgt gctgcgggcc tgctgatccg
      901 aagtctgacc aatctgcaga atcagcattt cggatttgag acgaagaacc gctacatcct
      961 gcatatcgac cctcagatgg ccgggtatca ggccaatcag gcggatacgt tcttccgcga
     1021 gatgcgcgat tcgctggcaa ccatacccgg cgtaaagcaa gtgacgtact cgctttacag
     1081 tccgatggaa ggcgacaact ggggggaggg ggtttacatc gaaggtcagg cgccgccgcc
     1141 gccgggaagt aacttcaaca acgcttcctg ggttcgcgcg agcgcgaact atttcgacaa
     1201 tatcggaacg aaaatcatag ccgggcgagg gatcacggag caggatactg gttcgacacg
     1261 tgcggtggcc gttgtgaatg agacgttcgc gaagcatttt ttcaaggatg gtcaggcgct
     1321 gggcaagcac tttggcgatc tcgatcagaa gtatgcggga aacttcgaga ttgtgggcgt
     1381 tacggaggac acgcagtact ggagccctat gagccgtatt cgccccatgt tctttcttcc
     1441 gggtccccaa tggcagcatt acagcgatcc ggccgaagtg atgttcgaga atgtgtcgca
     1501 tctggagatg aatgccgtcg agatccagac ggcaggatat gttccagggc tggagtcgca
     1561 ggtgcggcgc gtactctcgc agatcaatcc gaacctgacg atcatcagtt ttgagacatt
     1621 tgccgagcag gtgaaggagc gattcaacga tcaggaaatc atggtgcagc tgacgtcggt
     1681 atttggattc ctggccctgg cgctggctgc gatcggcttg tatggtgtga cagcctattc
     1741 ggtggcgcag cggacgagcg agattggaat ccgcatggcg ctgggtgcgg accggggcgg
     1801 gattctgcaa atggtgctgc gcagcgcctt tctgcaggcg ggcatcggat tggcgatcgg
     1861 gatgccggct gcgatctttg gcggtcgatt tatggcgagc atgctgtatc acgtgaagac
     1921 gtgggatccc gtggttttgg tcgtaaccac attgatcctt gcagttgcag cgtttctggc
     1981 ggccgtgatt ccggcgcagc gcgctgcaag cgtggagccg atgactgcgt tgcgagtgga
     2041 ataaggccga gaaaatccgg cacgggcagc cagacgcagc gatcagtgac agcaaggggc
     2101 taatatctct gtagagaggg cgtttgattt cggaatctct gcataaatgg ctcggacgtg
     2161 tcgcggatcg ggaagcgctg tgtgcatggc tgagccggct catgcttttg ctggttgcga
     2221 ttgaactggt caccatgccg ctgacccagc aactgtggac atgggacggc tttgtgcgcg
     2281 gggagcctga tttcgaactc ggcctgttca tggttgttgc ttgcctttgc ttgagtttgc
     2341 tgcgagcaca acacagccgg cagcggattt cattgctgct ggtgatcagc cgctggcttt
     2401 ctgaagttct tcgcgaacgc tttaaatccg tgttgctatg ggcgttgcta tctgcgcaaa
     2461 cgagccctga acatcttttt cctcgaactc ctcgtcgaat caccacgccg ctcctcatct
     2521 gatccgaggc cgtctggcgg ttttttgcct gggggccagg atgggtctgt tctccagccc
     2581 cgatcaccgg tttgaactgg aggagtcatg aagaagtccg aagtgctgag tgcatgggct
     2641 cgaattctgt ccggccgcgc accctctctg tcgatcgaga tcaccaagga atgtccgctg
     2701 cgatgtcccg gttgctacgc gttcgacgcg gcacacctgg ggggcgcgac ggaactgagg
     2761 gagctttccg atttccgtgg cgatgaactc gtc
//
LOCUS       DASZYN010000181         5670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_389481, whole genome shotgun sequence.
ACCESSION   DASZYN010000181 DASZYN010000000
VERSION     DASZYN010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389481"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VHE33_04565"
     CDS             <1..828
                     /locus_tag="VHE33_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HVT96756.1"
                     /translation="VEGVPGVAKTMTVKTLARFLALDFRRIQGTPDMMPADILGTSVF
                     SLKTSEFTFHRGPVFTQFLLTDEINRMPPRTQAALLECMEERQVTIDGETHTLDDFFT
                     VFATQNPLEFEGTYPLPEAQLDRFLIKIRVPYPNAAEERVVLERHHAKADTPQFEAIE
                     IAGVTAQDLVAARRELRAVRVEPALFDYLLAVVRRTREWPAITLGASPRAAANLLIVA
                     KAYAAKDGRDFLIPDDVKQAALPCLRHRIILKAEAELEGFDPDRVLSDVLAATPLPK"
     gene            825..2294
                     /locus_tag="VHE33_04570"
     CDS             825..2294
                     /locus_tag="VHE33_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HVT96757.1"
                     /translation="MIQTLIPAPVAAQAHSRGRFGRIFGTTLTARSLLLFLAGLLCAI
                     PGFFGLHWLALMLAWDAVVVVLVAVDILSLPPAHSFRVTRTFLDSPVLGRETRIELAV
                     EHEANAVLQAQIADDLHPALAQTPPSGAFQVFPRDPARITFTITPNRRGDFALRPIWL
                     RWRARLGLAERRALCDPAQKVRVYPTMERSADDTALYLLRIRQIELQRRRLRLTGTGR
                     EFDHLRDYRAGDEMRNISWPATARRAKVVTREFTTERSQQVWIVVDAGRLSGTVFEMR
                     RALSVGGRSNPASFRDAASDESFLLSLTQLDQACSAGIALAQTVMQAGDKAGLLVYGR
                     AVQQQLLPAAGAGALRIMIDALSQARVEGSEADHLRAAARLGQLQRRRSLVLWITEMA
                     DSARRPDVADAAADLARRHLVLLLVLSHPEIHATAAREPQTALQMFESAAAQEVIERR
                     RLVLAGLRSKGVLVVETTPGALRTDAINEYLEVKARGLL"
     gene            complement(2358..3350)
                     /locus_tag="VHE33_04575"
     CDS             complement(2358..3350)
                     /locus_tag="VHE33_04575"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HVT96758.1"
                     /translation="MISNRWIEQRQGLWQRLDALLVQTEQSGLKSLSGDELRELGLLY
                     RQVAQDLSAVRADRGSRTQEEYLNLLLARAHNRVYSGKKLEARQIVRFFAVEYPRIFR
                     RMFPYVVVSVAIFLAGAALGTLLSVARPAFERMILGPAMVASIEKHQMWTHSLVSMKP
                     QASTGIMTNNIGVAFATFAGGITAGLWTLYELFFNGLELGVVSTACAQARMALDLWSF
                     VAAHGALELPSICIAGAAGLRMATGMLFPGIHKRRESLARASVEAIRLLAGTVPLLVV
                     AGTLEAFLSPSGAPVAVKFLTGAVLFLALGLWLTSQGSGRRSQVAVVHPAETTR"
     gene            3390..4226
                     /locus_tag="VHE33_04580"
     CDS             3390..4226
                     /locus_tag="VHE33_04580"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HVT96759.1"
                     /translation="MDETPDQLTIETPEQVAIHFPIAGIGSRFLAVLADTVVQVIAYV
                     VIFLLIVLILTSAPRINGFMLSRVGDKWIIAAVILFYFLLYWGYFTLFEAFWNGQTPG
                     KRLCKLRVIRDSGRQITLFESMTRNLIRVVDAFPSFYAVGIVSMLCNRRNKRLGDFAA
                     GTLVVHERAPEPAPWSGTSSRTITAGAFTPVAVPPAPIVDPNAVDLPADAVARLTADD
                     LNVIDHFFARAIDIDIPRRRALAERLAQQMTAKMGIAMPGELQPERILESIARILRGI
                     RR"
     gene            4359..4748
                     /locus_tag="VHE33_04585"
     CDS             4359..4748
                     /locus_tag="VHE33_04585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HVT96760.1"
                     /translation="MATSSVTTSTPAPEVVPEPKTTPVRLTDNAVNKVREIMATQDPL
                     PAGLRIGVVGGGCSGFQYSMSFENQSGMMDKVYDFNGLKVFVDATSLMYLNGCTVDYV
                     ETLEAAGFKFDNPTVKSTCGCGSSFQV"
     gene            complement(4795..>5670)
                     /locus_tag="VHE33_04590"
     CDS             complement(4795..>5670)
                     /locus_tag="VHE33_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96761.1"
                     /translation="SIQRDKEMETIQRGEQYKRAIKLYYQKFGRYPTSIDQLVETNQV
                     RFLRKKYKDPLTGKADWKPVLFGQAHVRPLGFFGQPLMTVGLAGNASMYAVTASTTTD
                     ANGVPIASPDQPGGSASSNPSGFGSTFGSPSSSFGSPTSGIGSSPTSGSPTFGSMTTP
                     GSAPGSPTTSPFGSSGSGTSATTFGGGGPIVGFTLPIDKPSLIEYKQQKRYNKWEFNY
                     DPMEDQAMGIPGAGGVGSPNSTNPAGSILNPMGTTPGIGQSGTPSGFGTSGTSGTNPT
                     APTTPTMPPDTTPQQ"
BASE COUNT         1012 a         1989 c         1587 g         1082 t
ORIGIN      
        1 gtcgaaggcg ttcctggcgt agcgaagacg atgaccgtga agaccctggc gcgattcctc
       61 gccctcgact ttcgccgcat ccagggcact ccggacatga tgcccgccga cattctcggc
      121 accagcgtct tctcgctcaa gaccagcgaa ttcacctttc atcgcggacc cgtcttcacg
      181 cagtttctcc tcaccgacga aatcaaccgg atgccgccgc gcacgcaggc cgcgctgctg
      241 gagtgcatgg aagagcgcca ggtcaccatc gacggcgaga cgcacactct cgacgacttc
      301 ttcaccgtct tcgccacaca gaacccgctc gaattcgagg gcacgtatcc attgcctgaa
      361 gcgcagctcg atcgcttcct gatcaagatt cgcgttccct accccaacgc agccgaagaa
      421 cgtgtggttc tagagcggca tcacgccaag gcagatacgc cgcaattcga agccatcgag
      481 atcgccggcg ttaccgccca ggatctggtc gcggcacgcc gtgagcttcg cgctgtccgc
      541 gtcgagccgg cgctgttcga ttacctcctt gctgtggtgc ggcgcacacg cgagtggccc
      601 gccatcaccc tcggcgccag cccgcgcgcc gcggcaaatc tgctcatcgt agccaaagcg
      661 tacgccgcga aagatggccg cgactttctc attcccgacg acgtgaaaca ggcggcgctc
      721 ccctgcctgc gccaccgcat cattttgaag gccgaggccg agcttgaggg ctttgatccg
      781 gatcgcgtgt tgagcgacgt cctggcggcc acgcccctgc ccaaatgatc cagactctga
      841 tccccgcgcc ggtggcggcc caggcccata gcagggggcg cttcggaaga attttcggaa
      901 ccacgctcac cgcacgctcc ttgctgttgt tcctcgccgg actcctctgc gcgattcccg
      961 gttttttcgg cctccactgg ctggcgctca tgctcgcatg ggacgcggtt gtcgttgttc
     1021 ttgtcgctgt cgatatattg tcactgccac cggcacattc tttccgcgtt acccgcacgt
     1081 tcctcgattc gccggtgctg ggccgggaga ctcgcatcga actggcggtt gagcacgagg
     1141 caaatgcggt gcttcaggct cagattgcag acgatctgca tcccgcgctt gctcaaacgc
     1201 ctccatccgg cgccttccag gtatttcccc gcgatcccgc gcgcatcacg ttcacgatca
     1261 cgcctaatcg gcgcggcgat ttcgctctcc gccccatctg gctgcgctgg agagcgcgtt
     1321 taggcctggc cgagcgtcgc gccctctgcg atccggcgca aaaagtccgt gtttacccga
     1381 cgatggagcg ctccgccgac gatacggcac tgtatttgtt acgaattcgg caaatcgaac
     1441 tgcagcgtcg ccgcctgcgc ctcaccggta ccggccgcga gttcgaccac ctccgcgact
     1501 atcgcgccgg cgacgaaatg cgcaatatct cctggcccgc aaccgcgcgc cgcgccaaag
     1561 tcgtcacccg cgaattcacc accgagcgca gccagcaggt ctggatcgtg gtggacgccg
     1621 gacgcctttc cggaaccgtc ttcgaaatgc gccgagccct gtccgttggc ggccgttcga
     1681 atccagcgag ctttcgcgac gccgcgtcgg acgaatcctt cctgctcagc ctcacccagc
     1741 tcgatcaggc gtgcagcgcc ggcatcgccc tggcccagac cgtcatgcag gccggcgata
     1801 aggccggcct gctggtctat ggccgcgccg tccagcagca attgctgccg gctgccggag
     1861 ccggcgcgct ccgcatcatg atcgacgcgc tctcgcaggc gcgcgtcgaa ggctccgagg
     1921 ccgatcatct gcgcgccgca gcccggctcg gccagttgca gcgccgccgc agcctcgttc
     1981 tctggatcac cgagatggcc gacagcgcgc gccgccccga cgttgccgac gccgcagccg
     2041 atctcgcgcg ccgccacctg gttctgctcc tcgtcctcag ccatcccgag atccatgcaa
     2101 cagcagcacg cgaaccgcag acggctctgc agatgttcga gtctgccgct gcccaggaag
     2161 tgatcgagcg ccgccgcctc gttctcgccg gcctgcgctc caaaggcgtg cttgtggtgg
     2221 aaaccacgcc cggcgctctg cgcaccgacg ccattaatga atatctggaa gtgaaggccc
     2281 gcgggctgtt gtgaacgcag ggcgcagagc tcagatcgca gggctctaaa tcctgatgcg
     2341 agctctgatg ggaattctca acgagtcgtt tccgccggat gaacgactgc gacctgcgac
     2401 ctgcgacctg agccctgtga cgtcagccac agtcccagag caagaaacaa cacggccccg
     2461 gtaaggaact tcacagcaac aggcgcaccc gacggcgaca ggaacgcctc cagcgtgccg
     2521 gcgaccacca gcagcggaac cgttcccgcc agcaaacgaa tcgcctccac cgacgctcgc
     2581 gccagagatt cccggcgctt gtgaataccc ggaaacagca ttcccgtggc catacgcagg
     2641 cccgccgcac ccgcgatgca gatgctcggc agctccagcg ctccgtgcgc cgcaacgaag
     2701 ctccacaggt ccagcgccat gcgcgcctgc gcgcaggccg tgctcaccac gcccagctcc
     2761 agcccattaa agaacagctc gtacagcgtc cagagtcccg ctgtgatccc ccccgcaaac
     2821 gtcgcgaacg ccacgccaat attgttggtc atgatgccgg tggaggcctg cggcttcatg
     2881 ctcaccaggc tgtgcgtcca catctggtgc ttctcgatgg acgcgaccat cgccggcccc
     2941 agaatcatgc gctcaaatgc cggccgcgca acgctcagca gcgttcccag cgccgcaccg
     3001 gcaaggaaga tcgccaccga cacaacaacg taaggaaaca tgcggcggaa gatccgcgga
     3061 tactccacag caaagaagcg aacgatctgt ctcgcttcga gctttttgcc ggagtacacc
     3121 cgattgtggg ctcgcgccag cagcaggttc aggtattctt cctgcgtgcg cgacccgcga
     3181 tcggcgcgca ccgcggacaa atcctgggcc acctgccggt agagcagccc cagttcgcgc
     3241 agctcgtcgc cggaaagcga ctttagcccc gactgctccg tctgcaccag taacgcatcc
     3301 agacgctgcc acagtccctg ccgttgctcg atccagcggt tggaaatcat gctcgcgctg
     3361 cgcctcgaac cctgtcgatg aatcgcgcca tggacgaaac tcccgatcag cttaccatcg
     3421 agactccgga acaggtcgcg atccatttcc ccattgccgg cattggcagt cgcttccttg
     3481 ctgtactcgc agataccgtt gtgcaggtca tcgcctacgt tgtcattttc ctgctgatcg
     3541 ttctcatcct cacttcggcg cctcgcatca acggctttat gctctcgcgc gtgggcgata
     3601 agtggatcat cgccgctgtc atcttgttct atttcctcct gtactggggc tacttcacgc
     3661 tgtttgaggc cttctggaac ggtcaaaccc caggcaaaag gctctgcaag ctgcgcgtga
     3721 tccgcgactc cggccgccag atcacgctct tcgaatcgat gacccgcaac ctgatccgcg
     3781 tcgtcgatgc cttccccagc ttctacgccg tcggcatcgt ctccatgctg tgcaaccggc
     3841 gcaataagcg gctcggcgat ttcgccgccg gaaccctggt cgtccacgag cgagctccgg
     3901 aacccgcgcc gtggagcggc acctcctcgc gcaccatcac cgccggagcc tttacgcccg
     3961 ttgctgttcc tcctgcgccg atcgtcgatc cgaacgccgt cgacctgccc gccgacgccg
     4021 tcgcgcgcct gaccgccgat gaccttaacg tcatcgacca cttcttcgca cgggccatcg
     4081 atatcgacat cccccgccgt cgcgctctcg ccgagcggct cgcccagcaa atgaccgcga
     4141 agatgggcat cgccatgcct ggagaattac agccggagcg gattctggaa tccattgccc
     4201 gcatcctgcg cggaatacga cgctaaatcc tgcagaatgt gcacatttgg gcgatttcct
     4261 gcgacctcgg cgccatccgc cgcatccaat cttcaagggt gattctgtcc accgcgtgcg
     4321 gtacactcaa agggcagagg ttttccccag gagatttcat ggctaccagc tctgtcacca
     4381 ccagcacgcc cgctcccgaa gtagttcccg agccgaagac cacccctgtt cgcctcaccg
     4441 acaacgcggt gaataaggtg cgcgaaatta tggcaacgca ggatccgctg cccgccggtc
     4501 tgcgcatcgg cgtcgtgggc ggcggctgct ccggcttcca gtactccatg tcgttcgaga
     4561 accagtccgg catgatggac aaggtctacg acttcaacgg ccttaaggtc ttcgtggacg
     4621 cgacctcgct gatgtacctc aacggctgca ccgtcgacta cgtggagacg ctcgaagcgg
     4681 ccggcttcaa gttcgacaac cccaccgtca agagcacctg cggctgcggt tcgtcgttcc
     4741 aggtctaggt tttcaggtcg aagtgagaag gcccgccatc gtgcgggcct tttcttattg
     4801 ctgtggagtc gtgtccggcg gcatcgtggg ggtggtcggc gcggttggat tcgttcccga
     4861 tgttccagat gttccgaacc ccgaaggcgt tcccgactgt cctatgcccg gcgtcgtccc
     4921 catgggattg aggatcgaac cagccgggtt cgtactgttc ggcgatccga ctcccccggc
     4981 tcctgggatc cccatcgcct ggtcttccat cgggtcgtag ttgaactccc acttgttgta
     5041 gcgcttctgc tgcttgtatt cgatcagcga tggcttatcg atcggcagcg tgaacccgac
     5101 aatcggtccg cctccgccaa aggtcgtcgc agacgtgccg cttccgctgg atccgaacgg
     5161 cgaagtcgtc ggcgatcccg gagcagatcc tggcgtcgtc atggatccaa acgtcggcga
     5221 cccactggtc ggcgacgatc cgattcctga agtcggtgat ccgaagctcg aactcgggga
     5281 accgaaggtc gatccaaatc cagacgggtt cgacgaggcg ctccctcccg gctgatccgg
     5341 gctcgcaatc ggcacaccat tggcgtccgt cgtcgtgctc gcagtaacgg cgtacatcga
     5401 agcgttcccc gccagcccaa ccgtcatcag cggctgccca aagaatccca gcggccgcac
     5461 atgcgcctgt ccaaacaaca ccggcttcca gtcggccttg cccgtcagcg ggtccttata
     5521 cttcttgcgc aggaagcgaa cctgattcgt ctcgaccagt tgatcgatcg acgtcggata
     5581 gcgcccaaat ttctggtagt agagtttaat cgcccgcttg tactgctcgc cgcgctggat
     5641 cgtctccatc tccttatcgc gctggatcga
//
LOCUS       DASZYN010000182         7735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_198071, whole genome shotgun sequence.
ACCESSION   DASZYN010000182 DASZYN010000000
VERSION     DASZYN010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7735
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198071"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VHE33_04595"
     CDS             complement(<1..908)
                     /locus_tag="VHE33_04595"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVT96762.1"
                     /translation="MPSEVAAPSSTGLTTQWNPARAVLQKAVAEQAFPGAAYGVLHKG
                     SIVALDAVGRFTYEASSPTVKPDTVFDLASVSKVIATTAAAMVLVDRGKLDLDSRLGD
                     LLPGFVIGMAPGSGKERVTLRMLLAHTSGLPAYETLFRNCETPEALLRATLKLPLEAK
                     PGTRTEYSDFGFILLGKAIEILAYERLDAFCRREIFAPLNLTSTCFRPPRERRPTIPP
                     SENDQAFRHRVIQGEVQDENCSVLGGVAGHAGVFSNVPDLLAFAQCILARGRTRDGRD
                     LFQPETLDLFAQRQESNTGPSRALGW"
     gene            928..1515
                     /locus_tag="VHE33_04600"
     CDS             928..1515
                     /locus_tag="VHE33_04600"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HVT96763.1"
                     /translation="MRRRRCIGGHERAPAERCPDSRQPVRAPSLSRTRALRPIVLSAR
                     SWGPRVAQREEDHRQHPRFCCSGNAEIRSLASGLRVSGRIENLSLGGCRIRVENPRGF
                     REAERVEMTFCVRQLPLRVQASIRQLESGHTLGVAFTLLSERGKVQLGALIEELAETL
                     RDQVDTLAKYQSAEGGDKDSTRLARGPVPIDRDSR"
     gene            1597..2547
                     /locus_tag="VHE33_04605"
     CDS             1597..2547
                     /locus_tag="VHE33_04605"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein/thiosulfate
                     sulfurtransferase GlpE"
                     /protein_id="HVT96764.1"
                     /translation="MPIALDFFIKYGYGIVFLWVLTEQLGIPIPSTPLLLTAGTLTAT
                     HRLSLLYVLLSALAASLLADSTWYLFGKRYGSAVVRLMCRMSLESSACVRKTEGYFTK
                     GGPTALLVAKFVPGLGSVAAPIAGQINMGYGKFVLADAGGVLLWTLTVTLAGRFFGDF
                     LKHRPEALSTVAHFFGAIFALLFVGFLVWRMLQRRAFLRSVRMSRLDPQELKRMMDQG
                     QPVFIVDLRHPLDYLPDPRTLPGALSLTPDKLVEQSDRIPRDKEIVLFCTCPSEATAA
                     RMALRLRGMGIHRVRPLRGGYDEWKKLGYPLVDIATPTAA"
     gene            complement(2553..3722)
                     /locus_tag="VHE33_04610"
     CDS             complement(2553..3722)
                     /locus_tag="VHE33_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVT96765.1"
                     /translation="MRAVAEKLHLSEIAPGLVQSEIRAMTVACDLAGGVNLAQGVCDT
                     DVPSPVVARAIEAIQQGLNIYTRMDGIESLRQGIAAKLARHNRMTADPDAEILVTSGA
                     TAGLQAACMALFNPGDEVVLFEPFYGYHLNTLRALRVNAVSVPLNTPDWSLDIDALRR
                     SITPRTRGIVVNSPSNPCGKIFSRAELEAVAALAIENDLFVLTDEIYEYFVYDGAEHI
                     SLATLPGMAERTITISGLSKTFSITGWRIGYLIADRRWLGAIGYFHDLVYVCAPSPFQ
                     HASAAGLLQLPDSFYSGLSTEYQAKRDRLCAALTDAGLAPSIPQGAYYVLADVTNLPG
                     ATAREKARTLLRNTGVAGVAGTAFFAQGRGENLIRFCFAKRDEDLARACDLLRGL"
     gene            4748..6064
                     /locus_tag="VHE33_04615"
     CDS             4748..6064
                     /locus_tag="VHE33_04615"
                     /inference="COORDINATES: protein motif:HMM:NF017541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TROVE domain-containing protein"
                     /protein_id="HVT96766.1"
                     /translation="MARLNILKLAHLAPRTHEGAPAKRISEEQALRRSVLACLLWESQ
                     FYEDGVEIAGRIRELVPKVEAAKVAALAVEAREAMKLRHAPLYLVREMARHATHRALV
                     SETLARVIQRADELAEFVAIYWGEGRAPLSGQVKKGLAAAFPKFDEYALAKYDRAGAV
                     KLRDVLFLCHARPRDAAQAEVWKRLIAGELTTPDTWEVALSAGGDKRASWERLLAERK
                     LGALALLRNLRNMKDAGVSEDLVLGGLAAMKTERVLPFRFLAAARYAPQWEEVLEQTM
                     FRSVESRAKLAGRTVLLVDVSGSMTAALSGRSEMLRTDAAYGLAVLLREIAEEVSVYT
                     FSDTLKRVPARRGFALRDALDQSQPHSGTQLGAALEKLDERYERLIVITDEQSHDRVP
                     APRGRGYVVNVASYRNGVGYGAWTHIDGWSESVVEYIRELERAEAQ"
     gene            complement(6245..6937)
                     /locus_tag="VHE33_04620"
     CDS             complement(6245..6937)
                     /locus_tag="VHE33_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96767.1"
                     /translation="MIEAATQPDSSASTNAGGLAPEHRRCHYTRSTGHRCRDWALHGR
                     EHCFRHDRYLHALPDRPLHIPLLEDEDSITYVLSQALQALAWGTIPVSNGRAILNGCR
                     LAHTMQNRRLEAARLRLRARRLGIPEHEIFDTPPAKPEPEVPAANAAHQPDPANAESE
                     PTPNPQRERPKYLAFRDLKKNWDKELLRTENEMMDMAFKRNGETKEEFLAARATPFDN
                     LQKEETATAAAL"
BASE COUNT         1348 a         2185 c         2730 g         1472 t
ORIGIN      
        1 tcccatccga gcgctctcga cggtccagtg ttcgactcct gcctctgcgc gaacaaatcc
       61 agggtctccg gttggaagag gtcgcggccg tcgcgggtgc gacctcgtgc caaaatgcac
      121 tgcgcaaacg cgaggaggtc aggcacattg gagaagacac ccgcgtgtcc ggcaacgcca
      181 ccgaggacag aacaattctc gtcctgcact tctccctgga tgacacggtg gcgaaaggcc
      241 tggtcattct cgctcggcgg aatcgttggc cggcgctcgc gtggcggccg aaaacaggtg
      301 ctggtgagat tcaggggcgc aaagatctcg cgccggcaaa aggcatcgag ccgctcgtag
      361 gccagaatct cgatcgcctt gcccagcagg atgaatccga aatccgaata ctcggtccgg
      421 gtacccggct tcgcctccag aggcagcttc agcgttgcac gcagcagggc ctcgggggtc
      481 tcgcagttgc ggaacagcgt ttcgtaggct ggcaggccgg acgtgtgtgc gagcagcatc
      541 cgcagcgtca cgcgttcctt gccgctgcct ggcgccatgc cgatgacgaa tcctggcagg
      601 agatcgccca ggcgagaatc caggtcgagc ttgccgcgat cgaccagcac catggccgcg
      661 gcggtcgtcg cgatgacttt gctgacgctg gccagatcga agaccgtatc cggtttcacg
      721 gtcggcgaac tcgcctcgta ggtgaagcgg cccacagcgt cgagggcgac gatcgatccc
      781 ttgtgcagta ctccgtatgc ggctccggga aacgcctgct ccgcgacggc tttttgcagc
      841 accgcgcggg ctggattcca ttgggtagtc aagccggtgg atgagggcgc agcgacctcc
      901 gatggcatgg aaccagagta ctccgggatg cggcggcgcc gatgtatcgg tggtcacgag
      961 cgggcacctg cagagcgctg tcctgattca cgacagccgg ttcgcgctcc atcgctttca
     1021 cgaacccgcg ctctgcggcc gatagtcctt tcagcacgtt catggggacc acgggtggcg
     1081 cagagagaag aggatcacag acagcatccc cggttttgct gcagcggcaa cgcggagata
     1141 cgcagcctgg catcgggcct gcgcgtctcg gggaggattg aaaatctcag cctgggcgga
     1201 tgccggatcc gggtggagaa cccgcgcggg tttcgtgagg cggagagggt tgagatgacc
     1261 ttctgtgtcc ggcaactgcc ccttcgcgtc caggcgtcca ttcgccagct ggagtctggc
     1321 cacaccctcg gcgtcgcatt tacgctgctc tccgagcgcg gcaaggtcca gctgggggcg
     1381 ctgattgagg aactcgcgga gactttgcgt gaccaggttg acaccctggc gaagtaccaa
     1441 tcggccgaag gcggcgacaa ggactcgacc cgtttggcgc gagggccggt gccgatcgat
     1501 cgggactctc gctgaaaccg gccgcgccct tttgcctccc cgccacatct atactgaagc
     1561 tccgaactga agctcggaac ccccaggcgg cccacgatgc ccatcgcact cgacttcttc
     1621 atcaaatacg gctacgggat cgtgttcctg tgggtgctga ccgagcaact gggcattccc
     1681 atcccgagca cccccctgct gctgaccgcc ggcacgctaa cggcgacgca tcggctcagc
     1741 ctgttgtacg tgctgctctc ggcgctggcc gccagtctgc tggcggacag cacgtggtac
     1801 ctgttcggca agcgctatgg gtcggccgtg gtgcggctga tgtgccgcat gagtctggaa
     1861 agctcggcgt gcgtgcgcaa gaccgagggc tacttcacca agggcggacc cacggccctg
     1921 ctggtggcca agttcgttcc cggcctgggt tctgtggctg cgccgatcgc cggccagatc
     1981 aacatgggct acggcaagtt cgttcttgcc gatgcaggcg gcgtgctgct gtggaccctc
     2041 actgtgacac tggccgggcg tttcttcggc gacttcctca agcaccggcc ggaagcgctc
     2101 tcgacggtgg cccacttctt cggagcgatc ttcgcgctgc tcttcgtcgg cttcctggtc
     2161 tggcgcatgc tgcagcgacg cgcattcctg cggtcggtgc ggatgtcgcg cctcgatccc
     2221 caggagctga agcggatgat ggaccagggc cagccggtgt tcatcgtgga cctgcggcat
     2281 ccgctggact acctgcccga tccgcgtacg cttcccgggg cgctgagcct gacgccggac
     2341 aagctcgtgg agcagagcga ccggattccg cgagacaagg agatcgtgct gttctgcacc
     2401 tgccccagcg aggcgacggc ggcgcggatg gcgctccggc tccgtggaat gggcatccac
     2461 cgggttcggc cgctgcgggg cgggtacgac gagtggaaga agctgggcta tccgctggtg
     2521 gacatcgcaa cgcccaccgc cgcctgacag cttcagagcc cgcgcagcag atcgcaggct
     2581 cgcgccagat cctcgtcgcg cttggcgaag cagaagcgga tcaggttctc tccgcgaccc
     2641 tgggcgaaga acgccgttcc cgccacgccg gcgacgccgg tattgcgcag cagggtgcga
     2701 gctttttcgc gcgcggtcgc gcccggcaaa tttgtaacat cggcgagcac ataataggca
     2761 ccctggggaa tcgatggagc tagtcctgcg tccgtcaggg ctgcgcagag cctgtcgcgt
     2821 ttcgcctggt actcggtgga gaggccggag tagaacgaat cgggcagttg cagcaggccc
     2881 gctgcagagg cgtgctggaa cggcgagggc gcgcagacgt agacgaggtc gtggaagtag
     2941 ccgatggctc cgagccagcg gcggtcggca atcaaatatc cgatgcgcca gccggtgatg
     3001 ctgaaggtct tcgacaggcc ggagatggtg atggtgcgct cggccatgcc tggcagcgtg
     3061 gcgaggctga tatgctcggc gccgtcgtag acgaagtact cgtagatctc gtcggtgagg
     3121 acgaagaggt cgttctcgat ggcgagggcc gcgacggctt cgagttcggc gcgggagaag
     3181 atcttgccgc acggattcga aggggagttc acgacgatgc cacgagtgcg cggggtgatg
     3241 gagcggcgca gggcgtcgat gtcgagcgac cagtcggggg tgttgagcgg aaccgagacg
     3301 gcgttgacgc ggagagcgcg cagcgtgttc aggtgatagc catagaaggg ctcgaagagg
     3361 acgacctcgt cgcccggatt gaagagggcc atgcaggcgg cctgaagccc ggcggttgcg
     3421 ccggaggtga cgagaatctc ggcgtcggga tcggcggtca tgcggttgtg gcgcgcgagt
     3481 ttggcggcga tcccctggcg caggctctcg atgccgtcca tgcgggtgta gatattgagg
     3541 ccctgctgga tcgcttcgat ggcgcgagcg acgaccgggg aaggcacgtc ggtgtcgcag
     3601 acaccctggg ccaggttgac gccgccggcg aggtcgcagg ccacggtcat ggcgcgaatt
     3661 tcggactgaa cgaggccggg cgcaatctcg ctgaggtgca gtttttcggc tacggcacgc
     3721 atgggttcac gtttaccaca atcggatgtc gtttcgcatc tgcgcattgt gatcgtaact
     3781 tcacaggccg atccgaacga agttagaaaa tctttgtaat ttgtcacaac attccttgct
     3841 ttttcgggct gaattatggc agagtattct ctgcacagga gacattccat gcgagtcaaa
     3901 acgcataatt cgattggatg gctgccacag gcggacgtct gcgtcggttc cggaatgtcc
     3961 tttctggcgt ttcgatagcc tcccggctaa tccgatcctg tgcattccat acaaagcctc
     4021 cactgagtgg cgaagaagac agttccttcg acttgacacc gaatacggct gtcttcggct
     4081 gatccctcag tgactcataa ccaggcaccg gtggcgacag aagggagtta cttccgccaa
     4141 agaaaccgag cagggacgga aaccctgcca gagtcgagag attccgggac gggggcgtag
     4201 ccccgcagca caaggtaccg gcatcaggat ggctctgaag actcttttcg actaatcccc
     4261 ggtgtaagta aaaatttggc cggtggcgca gataacagtt acttctcctt aattcgacgg
     4321 ttgcgagttc gaatctcgcc gcgggcataa gcccgcgtag cttaatggca gagcagcgaa
     4381 acaagtctgt tatcgactta ttcccaggcc attcatagtg cgtcgccggt ggcgcagagg
     4441 acggatccat caacggtagc aatgcaggtt cgagtcctgc cgtggatcgg tgcaataagt
     4501 gctcaagatc agcgtagcaa tccgtcatcg aatattcccc ggtgtcgacg tcaaccagca
     4561 accgcaacac taaacaaccc gacaataacc gcgccggtgg cgcagacaac ggatccttca
     4621 atggcagaag cttcgcacgc gcgtgcggag agtgcaggtt cgactcctgc cgcggacgca
     4681 agtccgtgtc aaattcccgt cgtcgtttat tccccggcgt tagcatggca gtcgaaagga
     4741 gggacccgtg gcacggttga acattctgaa gctggcacat ctggcgccgc gcacccatga
     4801 gggcgcgcct gcgaagcgga tcagcgaaga gcaggcgttg cggcgcagtg tgctggcctg
     4861 cctgttgtgg gagagccagt tctacgagga cggagtggag atcgccggcc gcattcgcga
     4921 actggtgccg aaggtggagg ctgcgaaggt ggccgctctg gccgtggagg cgcgcgaggc
     4981 gatgaagctg cgccacgcgc cgctgtatct cgtccgcgag atggcgcggc atgcgacgca
     5041 tcgcgccctg gtgtcggaga cgctggcacg cgtgatccag cgtgccgatg agctggccga
     5101 gttcgtggcc atctactggg gcgaggggcg cgctccgctc tcgggccagg tgaagaaggg
     5161 cctcgcggcg gcgttcccga agttcgacga gtacgcgctg gcgaagtacg atcgcgccgg
     5221 agcggtgaag ctgcgcgacg tgctcttcct gtgccacgcg cgtccgcgcg acgcagctca
     5281 ggccgaggtg tggaagcgcc tgatcgcggg cgagctgact acgccggaca cctgggaggt
     5341 ggcgctctcg gccggtggcg acaagcgcgc cagctgggag cgtttgctcg ccgagcggaa
     5401 gctgggggcg ctcgctctgc tgcgcaacct tcgcaacatg aaggatgcgg gcgtgagcga
     5461 ggacctcgtg ctgggcggac tggccgcgat gaagaccgag cgcgtgctcc cgtttcgctt
     5521 tctcgctgct gcgcgttacg caccgcagtg ggaggaggta ctggagcaga cgatgttccg
     5581 ctcggtggag tcgcgcgcga agctggcggg ccggacggtg ctgctcgtgg acgtgtcggg
     5641 cagcatgacg gcggcgctct cgggccggtc ggagatgctg cgcaccgatg cggcgtacgg
     5701 tctcgcggtg ctgttgcgcg agatcgcgga ggaggtgagt gtctacacct tttccgacac
     5761 gctgaagcgg gtgccggcgc ggcgcggctt tgcgttgcgc gatgcgctcg accagagcca
     5821 gccgcacagc ggcacgcagc tgggcgcggc gctggagaag ctcgacgagc ggtacgagcg
     5881 actgatcgtg atcaccgacg agcagtcgca cgatcgcgtg ccggcgccga ggggccgggg
     5941 ctacgtggtg aacgtggcca gttaccgcaa cggcgttggc tacggcgcgt ggacgcacat
     6001 cgacggctgg agcgagtcgg tggtcgagta catccgcgag ctggagcgtg cggaggcgca
     6061 gtaaggctga ggggccggcg gggaactcgc tggcctttcg gcgatcttgc gagcggtggg
     6121 cgggttcgtt tggggtctcc tgcaaaaagt attttgaggg ggggtgaccc ctcgggatgt
     6181 tgttgattcc cggaccaggg ttttgatcgg accccggggg tgttaacgat gtggttcaca
     6241 ggcatcagag cgccgcggcg gtggcggttt cttctttctg caggttgtcg aagggagtgg
     6301 cgcgggcggc gaggaattct tctttggttt cgccgttgcg tttgaaggcc atgtccatca
     6361 tctcgttctc cgtgcggagg agttccttgt cccagttctt tttgaggtcg cggaaggcga
     6421 gatatttggg acgctcgcgt tgagggtttg gggttggttc ggactcggcg ttcgctggat
     6481 cgggttggtg cgcggcgttg gcggccggga cttcgggctc gggcttcgca gggggcgtat
     6541 cgaagatttc gtgttcgggg atgccgaggc ggcgggcgcg gaggcggagg cgcgcggcct
     6601 cgaggcggcg gttctgcatg gtgtgggcga ggcggcaacc gttgaggatg gcgcggccat
     6661 tcgaaaccgg gatggtgccc caggcgaggg cctgcagagc ctgagagagg acgtaagtga
     6721 tggagtcctc gtcttcgagg agggggatgt ggagcgggcg gtcggggagg gcgtggaggt
     6781 agcggtcgtg acggaagcag tgttcgcggc cgtggagggc ccagtcgcgg cagcggtggc
     6841 cggtgctgcg ggtgtagtga cagcggcgat gctcaggggc gaggccgccg gcgttggttg
     6901 aggcggagga atcaggctgg gtcgcagctt cgatcatgag ggtgtccttt gcgttgtgga
     6961 ggccaggttg gggtgcggat tcggcctcgg aaaccgggcg gttcggggcg agctcggatg
     7021 tggagtcgat tcgctgtttg tgcgccatgt tgtgggcgat cttgtgcttt ttacggggta
     7081 caagttataa gtcctttgtt tctgagatga tagcgatgat gactagtttg acaactttgg
     7141 caggtccggg tgatttgcgg tacttcggtc ctgttggcgt gggtggatgc atggagccct
     7201 ttcgtggcag cagcgttcag ttttcagcgt tcagcaaggc tgcgcgcctt gccggcgcgt
     7261 gcattgcggg cggcaataaa aaagcctcac cggatgcggt gaggcttttt cagggaacac
     7321 tttttccctg ttgtcaggct aggctttggc gggggtggat gcaagggaaa tcggagggtg
     7381 ggacggtacg aaggtcatga tccacctgcg atcacgatga aggcaatgag aagttgcgaa
     7441 gcgcgagcgc tgactgaccg cttcctatac tccctttagt aacccagcag gcatgattca
     7501 gcagggcgaa cgctccaagt gacgccgttc gattgcaaat cgccggatga ccgggattgc
     7561 gaaagctatt cgaaagactc ttcagcggat ttcgggcgtg gaaacgtggg aaacacgaat
     7621 ccgctttcaa gccaccccag cgaaacgttg ttttcgctgg gaccccgggt gcccgccctc
     7681 gcgccctggc atttcctcgc aagcgaggaa atgccaccgg gtttcggagg aggtc
//
LOCUS       DASZYN010000183         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_714139, whole genome shotgun sequence.
ACCESSION   DASZYN010000183 DASZYN010000000
VERSION     DASZYN010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714139"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VHE33_04625"
     CDS             complement(<1..656)
                     /locus_tag="VHE33_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HVT96768.1"
                     /translation="MVLLWQFSPFRLTLTGVANPPTCLPMKPARLVLTPTRSRRLNEL
                     IGLLVLASAVLLFLSLVSWHPSDPSLNTVGSGRVHNWIGPAGSYLSDIMLQIEGISAF
                     ILPVLLGALGWTWMLSRPAGSPAFKTIGIILIFFFAPALFALLPGHPTFLYGLPVAGL
                     IGRLVTDFLIRFLNYPGACIVTVTLVAAAIYLSTTFSFNTAREWLGIKLAFFLAWRDR
                     "
     gene            817..993
                     /locus_tag="VHE33_04630"
     CDS             817..993
                     /locus_tag="VHE33_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96769.1"
                     /translation="MLRDRGVCVARVVPVDPDYVSDHEVRLVAEGKLKLPERELDWGE
                     FFSLAKGGKACLSG"
     gene            complement(1462..>2119)
                     /locus_tag="VHE33_04635"
     CDS             complement(1462..>2119)
                     /locus_tag="VHE33_04635"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HVT96770.1"
                     /translation="IRNHPLTLTLVAFVCTAIPALALTPVIGRHLESLRVMALSLVIG
                     GIVMWIVDAWSVRRDLLVSDVEQMSLPQSIWIGLCQVTSAVFPGTSRSMSTIAAGQLA
                     GMTRPAALEFSFLVSIPTMFAATLYDLLKAVFPDLHLLHRQHGAAAASHAALAPLAMT
                     GHGWLVLIVGFLVSFFVALGVVEWFLQWVRNHGFVLFAVYRLLLGASLLIFGGRFMAT
                     "
BASE COUNT          512 a          517 c          786 g          304 t
ORIGIN      
        1 aagcggtcac gccaggcgag gaagaaagcg agtttgatgc cgagccactc gcgtgcggtg
       61 ttgaagctga aggtggtcga caggtagatg gccgcagcga cgagggtgac ggtgacgatg
      121 caggcgcccg gatagttgag gaagcggatc agaaagtcgg tgacgaggcg tccgatgagg
      181 ccggcgacag gcagtccata gaggaatgtc ggatggccgg gcaggagggc gaagagggcg
      241 ggcgcaaaaa agaagatgag gataatgccg atggttttga aggcgggcga gccggcagga
      301 cgagagagca tccacgtcca gccgagggca ccgaggagga ccggcaggat gaaggcggag
      361 atgccttcaa tctgcagcat gatgtcgctg aggtacgagc cggcggggcc gatccagttg
      421 tggacgcgcc cggagccgac ggtgttcagg gaggggtcgc tggggtgcca ggaaaccagg
      481 gagaggaaaa ggaggacggc ggaggcaaga accagcaggc cgatcagctc attgagccgg
      541 cggctgcggg taggcgtaag caccaaacgg gcgggtttca tgggcagaca ggtcggggga
      601 ttcgccacgc cggtgagggt gaggcgaaac gggctgaact gccagagcag aaccattatc
      661 ccagatgcgg aggcgggtgg gagacaaaag ggggtggggg aatcggggtg ggtatggcgg
      721 tgtgggattc gattggaaag atgcagttgt cacgcttgcc caatttaaaa acgcctgagc
      781 caccatcttg cgcttgtgcg caaaggtgaa gggtttgtgt tgcgcgaccg gggcgtctgc
      841 gtggcgcggg tggttcctgt cgacccggac tatgtttctg accatgaggt gcggctggta
      901 gcggagggaa agctgaagct gccggagcgt gaattggact ggggtgagtt tttctctctg
      961 gcaaaggggg gaaaggcatg cctcagcggc tgaagccgac agtattcgcg gcctctactg
     1021 cccggctgaa gccaggccct ttcaaagaag cgcaggtcac aacgaaaatg cccagttcgc
     1081 gctgagttgg ttggctgcac tgttcacgaa gagcgccgcc cctctcgatg aacagctaag
     1141 gctgcgagcg aggcaaacat gatcatcaac aagtcgaccg agtgtgtgtg gagaaaccag
     1201 ccgagggggc ggaggggaac gcctgtcctg atcaaggcgc aggcgacgaa atttgcgatg
     1261 caggcagcaa ccaggaggaa tggccgctga ggggaaagtt tcaatccagc caaaacaagg
     1321 agcaacaaac ccacgagccc gacgatcggc agcgcaaatc ctgctatcgc ggtggccgtt
     1381 gcacaccaga aactttccgc ggcgacgcaa accatcacga gaatcaggga gaggcaggaa
     1441 gtttcacgag ctataccttg ctcaggttgc cataaaccgt ccgccgaaga tcaaaagcga
     1501 agcacccagc agcaggcggt agacggcgaa gagaacgaag ccgtggttgc ggacccactg
     1561 cagaaaccac tcgacgacgc cgagcgcaac gaaaaatgag acgaggaagc cgacgatgag
     1621 gaccagccat ccgtgtccgg tcatggcgag cggggcgagc gccgcgtggg aggcggctgc
     1681 agcgccatgc tgccggtgca ggagatgaag gtccggaaag acggccttga gcaggtcgta
     1741 gagtgttgcc gcgaacatgg tcggaatcga gacaaggaag ctgaactcga gagcggcagg
     1801 gcgggtcatg ccggcgagtt gaccggcggc gatggtggac atggagcgcg aggtcccggg
     1861 aaagacggcg gaggtgacct ggcaaaggcc gatccagatc gactgcggaa gggacatctg
     1921 ctcgacgtcg gagacgagga ggtcgcgacg gacactccag gcatcgacga tccacatgac
     1981 gatgcctccg atgacgaggg acagggccat gacgcggagg ctttccagat gcctgccaat
     2041 caccggagtg agagccagcg cgggaatcgc ggtgcaaacg aaggcgacca gagtcagcgt
     2101 gagcggatgg ttgcgaatc
//
LOCUS       DASZYN010000184         4195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_775204, whole genome shotgun sequence.
ACCESSION   DASZYN010000184 DASZYN010000000
VERSION     DASZYN010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775204"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..1624
                     /locus_tag="VHE33_04640"
     CDS             203..1624
                     /locus_tag="VHE33_04640"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HVT96771.1"
                     /translation="MSIRVKALICAVVAGAIASLSALRGHPHQQWIHFFIYLAAILLS
                     SGMKVTLPKSDGTMSVNFPFIFLGILQLSPLQAVCLGVCSVAAQCRFKVRQPFTLVQI
                     LFNVANVTTATALAWFTYAGAMKAHPESAPVLALAATVYFLANTIPVALVIGWSSDTS
                     PFMHWRQAFLWYLPFYLVGAMVAVLADFIGIRYGWLTSLLLIPLVYTVYRAYCAQMAM
                     LRDRERHIEEVESLYMRTIEGLSMAVEAKDEGTHEHLMRVRVYVAELGRIMKLDRPLM
                     QALNIAAFLHDIGKLAVPEQILNKPGKLTPEEFEKIKIHTVVGADILERVKFPYPVVP
                     IVRAHHEAWDGSGYPDGLKGEEIPIGARILTAVDCFDALVSDRPYRRALPPEEAMNFL
                     KSKSGIQFDPAIVTLLQEHYPALEELARKHSSEMDPLRTDVFIERGAAPAAGFEVESA
                     ESKTAGEHGQETPNPVLAGMPAS"
     gene            1621..2478
                     /locus_tag="VHE33_04645"
     CDS             1621..2478
                     /locus_tag="VHE33_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVT96772.1"
                     /translation="MNPALSFLHWMGRTLAIILLVLLGSTALVRYAPGYLSDAREMDS
                     RYAAEAHTELSAEAARSRSIPQMVAAEIAGWIRGDFGTSRQYEVPVSELIGPRIAASA
                     GLLLRGIGVAWILALCAALIASAGRNPSFLWQTPATLLLAIPTAALATFCLLADSGGP
                     VLVMALLLAARDFKFLDQVLRKAWREPHILQARAQGIGTGRLLVAHLAPSLVPQLGAL
                     ASLSIVTALSALVPVEVIFNVPGLGQLAWNAALNRDLPVLLAVMILMALAVTFSGTAA
                     ARAEGWKSA"
     gene            2475..3263
                     /locus_tag="VHE33_04650"
     CDS             2475..3263
                     /locus_tag="VHE33_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVT96773.1"
                     /translation="MKLRRSLLALLAVLIVSASAIIALPGASYARQDREATLASASLH
                     HLAGTDALGRDRLVRVAAAFLLSLAGAIAASAVTTAAAAGVGMLAALGRSAMGWAVML
                     ICDVFLALPWMFLLMMVRSALPLTTSPGRSAAITFLVLAALGWPASARAVYRGVVQLK
                     TSDWMLYARASGLGGVSVARHIIPNLFPLLLPQFLISIPAFVMAEANLGALGLGIGEP
                     LPSWGGMLFELNNSPMLMQTRWVYFPVILLVLVLLLLEGVAAEA"
     gene            3268..>4195
                     /locus_tag="VHE33_04655"
     CDS             3268..>4195
                     /locus_tag="VHE33_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVT96774.1"
                     /translation="MYLSRRILVCFAALLPIAPAAMSAAQTAPRLPGELAWTIGYDPK
                     TFDPAKVDDQESETVRYLTAGVLLRFNRLTQKVEPELAKSWTLSPDGKTLTFTLRSGL
                     EFSDGSNLTARDVAASVRRVLSLGDEAPASQEFIKPAGVTVDSPNDSTVVVHLPQRVI
                     AIGKVFDEIAIEPAGHSSEARVTSGPFYVSDYHRSRYIRLRRNPHYVGSNGTQPSATG
                     IRLDILENMEQQIRLLQRGDYDLIDSLPPDYFELLRKKAPGMARDLGPSLNTEQMWFN
                     QSPASPLPAWEKTWFQNRGFRVAVSQAIHRSDL"
BASE COUNT          702 a         1376 c         1216 g          901 t
ORIGIN      
        1 cgttccgaaa tgagacagat cgatgacaac tcagggcttg cggccttccc cctgggtcga
       61 tcttgcaact aaagacgcat tgggaatgac ctggtggact cggtaccatg cagctggacg
      121 taacttcgga accacaggtg gaacagcgag tctcctgcag ggactcagta cggctgtaaa
      181 cgaccagtca aggtagagca tcgtgtcgat ccgtgttaag gctctcatct gcgccgttgt
      241 ggcaggggcc atcgcgagtt tgtccgccct ccgcggccac cctcaccagc agtggatcca
      301 cttcttcatc tacctggcag ccatcctcct cagctccggc atgaaagtga ctctgccgaa
      361 gagcgacggc acgatgtcgg tcaacttccc ctttatcttt ctgggcatcc tgcagttgtc
      421 tcccctgcag gcggtctgcc ttggcgtgtg cagcgttgcc gcgcagtgcc gcttcaaggt
      481 aaggcagccg ttcaccctgg tgcagattct gttcaacgtg gccaacgtga ccacggcgac
      541 ggctttggcg tggtttacat atgctggtgc gatgaaagcc catcccgaga gcgcgcccgt
      601 gctcgcgctt gccgcgaccg tctactttct ggctaatacc attcccgtgg cactggtcat
      661 cgggtggtct tccgatacct cgccgttcat gcactggcgt caggctttcc tgtggtatct
      721 gccgttctat ctggtgggcg ccatggtcgc cgtgctggcc gacttcatcg gaatccgcta
      781 tggctggctc acttcgttgc tgttgatccc gctcgtttac acggtctacc gggcttattg
      841 cgcgcagatg gccatgctgc gcgatcgcga gcggcacatc gaagaggtgg aatccctcta
      901 catgcgaacg atcgaagggc tttcgatggc ggttgaagcc aaggacgaag gcacgcacga
      961 gcacctgatg cgagtccgtg tctacgtcgc ggaactgggc cgcatcatga agctcgaccg
     1021 accgctgatg caggccctga atatcgcagc tttcctccac gatatcggca agctggcagt
     1081 tccggagcag atcctgaaca agccgggcaa actgactccg gaagagttcg aaaagatcaa
     1141 aattcatacc gtggtcggag ctgacattct cgagcgcgtg aagttcccct atccggtcgt
     1201 gccgatcgtc cgcgcgcacc acgaagcgtg ggacggcagc ggttatcccg atggcctgaa
     1261 gggtgaagag atccccatcg gcgcccgcat cctgaccgcg gtcgattgct tcgacgctct
     1321 cgtctccgat cgtccctacc ggcgcgccct gcctcccgag gaagccatga acttcctcaa
     1381 aagtaaatcc ggaattcagt tcgatcctgc gattgtgacg ctgctgcagg agcactatcc
     1441 cgcgttagag gagcttgccc gcaagcacag cagcgaaatg gatccgctcc ggaccgacgt
     1501 cttcatcgag cgtggcgctg ccccggccgc gggcttcgaa gtggaatcgg ccgaatcgaa
     1561 aacggccggt gaacatggtc aggaaacacc caatcctgtt ctggccggga tgcccgcgtc
     1621 gtgaacccgg cactttcgtt ccttcactgg atgggacgaa cgctcgccat catcctgctg
     1681 gtccttcttg gttctacggc tctggtgcgt tacgctcccg ggtatctaag cgacgcgcgc
     1741 gaaatggata gccgctacgc ggccgaggcg cacaccgaac tctcggccga agccgctcgc
     1801 agccgttcga ttcctcaaat ggtcgccgca gagatcgccg gctggattcg cggagacttc
     1861 ggcacatcgc gtcagtacga agttcctgtt tcagaactga tcggtccacg gattgccgcc
     1921 agcgccggtc tgctgcttcg cggcatcggc gtcgcatgga tcctggcgct atgcgctgcg
     1981 ctgattgcga gcgcgggacg gaatccatcc ttcctctggc aaacgccggc cacgcttctg
     2041 ctcgcgattc ccaccgccgc tcttgccacg ttttgcctgc tcgcggacag cggcggaccg
     2101 gtgctggtca tggcgcttct gctcgccgcg cgcgacttca aattcctcga tcaggtcctg
     2161 cgcaaagcct ggcgggaacc ccacattctg caggctcgcg cacagggtat cggcaccggg
     2221 cgtctgctcg tcgcgcatct ggccccgtca ctggttccgc agctgggcgc tctggcttct
     2281 ctctcgatcg tgacggctct cagcgcttta gttccggtag aagtcatctt caatgttccg
     2341 gggctcgggc agctggcctg gaatgccgct ctcaaccgcg acctgcccgt gcttctggcg
     2401 gtgatgattc tcatggcgct ggcagtcacc ttctcgggca ccgctgcggc acgcgcagag
     2461 ggatggaaaa gcgcatgaag cttcgccgct ccctcctcgc tctccttgct gtgctcatcg
     2521 tcagcgcctc tgcgatcatc gccttgcccg gcgcaagcta cgcccgtcag gatcgcgaag
     2581 cgacactggc atccgcatcg ttgcatcacc ttgccggcac cgacgcgctg ggacgcgacc
     2641 ggctcgtgcg cgtagcggct gcatttcttc tcagcctcgc gggcgccatc gccgcctccg
     2701 ctgtcaccac cgcagccgct gccggtgttg gaatgctcgc agcactcggc cgctcggcga
     2761 tgggatgggc cgtgatgctg atctgcgatg tctttctggc gctgccatgg atgttcctgc
     2821 tgatgatggt gcgctcggca ctgccactga ccacctcgcc gggacgttcc gccgcgatca
     2881 catttctggt gctggctgcg ctgggctggc cggccagcgc gcgcgccgtc tatcgcggtg
     2941 tagtgcagct gaagacttcc gactggatgc tgtacgcgcg cgcctcggga ctcggcggcg
     3001 tcagcgtcgc ccggcatatc atccctaatc tgtttccgtt gctgctgccg cagttcctca
     3061 tcagcattcc tgcgttcgtg atggcggaag ccaatcttgg agcgcttggc ctcggcatcg
     3121 gcgaaccgct gccctcgtgg ggcggaatgc tcttcgagct gaacaattcc cccatgctca
     3181 tgcagacccg ctgggtttac tttcctgtga ttctgctggt gctggtgctg ctccttctgg
     3241 aaggagtggc cgctgaggcc tgatcgcatg tacctttctc gccgcattct cgtctgcttc
     3301 gccgccctgc tgccgatcgc gcccgctgcg atgtccgcag ctcagaccgc gccgcgcctg
     3361 cccggagagc tggcctggac cattggctac gatccgaaga ctttcgatcc ggcaaaggtt
     3421 gacgaccagg aatcggagac ggttcggtat ctcactgccg gtgttctcct ccggttcaac
     3481 cggcttacgc aaaaagtgga gcccgagctc gcgaagagct ggaccctctc tccggacgga
     3541 aagaccctca cgttcaccct gcgctccggt ctcgagtttt ccgatggcag caacctaacg
     3601 gcgcgcgacg tcgcagcatc ggtgcgccgc gtcctttccc tgggagatga ggcgccagcc
     3661 agtcaggaat tcattaagcc tgcgggcgtc accgtggact cccccaacga cagcacggtc
     3721 gttgtccatc tgccgcagcg ggtgattgcc attggcaaag tcttcgacga aatcgccatc
     3781 gagcctgccg gccactcctc ggaggcgcgc gtcacctccg gtccctttta cgtgtccgac
     3841 tatcaccgtt cccggtacat tcgtctccgc cgtaacccgc attacgttgg atccaatggc
     3901 acccagcctt ccgcaaccgg cattcgcctc gacatcctgg agaacatgga gcagcaaatt
     3961 cgtctcctgc agcgcggcga ctacgacctg atcgacagtc ttccccccga ctacttcgag
     4021 cttctgcgaa aaaaagctcc gggaatggcg cgcgatcttg gcccgtctct caatacagag
     4081 caaatgtggt tcaatcagtc gccggcttcc cccctccctg cgtgggaaaa gacctggttt
     4141 cagaatcgcg gctttcgcgt agccgtctcg caggcgattc atcgttccga cctcg
//
LOCUS       DASZYN010000185         5789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1228392, whole genome shotgun sequence.
ACCESSION   DASZYN010000185 DASZYN010000000
VERSION     DASZYN010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228392"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..821
                     /gene="nadD"
                     /locus_tag="VHE33_04660"
     CDS             126..821
                     /gene="nadD"
                     /locus_tag="VHE33_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HVT96775.1"
                     /translation="MLKSMKIGLFGGSFDPPHRGHVALARLAIESVGLDRVLFAPVGV
                     QPLKRDRPQASFADRLEMTELAIAGERGMELSQIDAPRSDGRPNYTIDTVRLLLGTLA
                     ADDELYCLMGADSFLSIGKWHRAADLLVACNFVVAARPGFDLGRITSALPASIAVAAC
                     DGAGAENLVLCLHGAGESESRLYLLPHLQERASASGIRRSLQEGAEPRWDLAPAVAAY
                     IAEHRLYQNSRAS"
     gene            886..1413
                     /gene="rsfS"
                     /locus_tag="VHE33_04665"
     CDS             886..1413
                     /gene="rsfS"
                     /locus_tag="VHE33_04665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HVT96776.1"
                     /translation="MTLTETRKMVLAAVAACDEKKGEDTRVLQLDSADSAFTDFFLIT
                     STANDRQSQAIADEIELRLKRDFGTYPNSVEGRRAGEWILMDYVDFVVHIFLRDKRSF
                     YDIERLRKSARTIELAELTAALTEKTLAARKKAPVKRAAKKAVTKKATAKKAAKKSAG
                     KNIKPAAKSAGKRGK"
     gene            1418..2506
                     /gene="dcm"
                     /locus_tag="VHE33_04670"
     CDS             1418..2506
                     /gene="dcm"
                     /locus_tag="VHE33_04670"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HVT96777.1"
                     /translation="MPRAAFTFIDLFAGIGGMRMGFEAAGGRCVFTSEWNPFARKTYA
                     ANFGLSEAIAGDIREQDENEIPDHDVLVAGFPCQPFSIAGVSKKNALGRPHGFACATQ
                     GTLFFDVARIIAAKRPRAFLLENVKNLVNHDRGRTFRVIRQTLKEELGYCISWRVIDA
                     VHFVPQHRERILIAGFREECKFDLDQVKLPAAAPKLGSVLHPEDGSERPEEPYTLAPR
                     GGVNAKYTLSDKLWAYLQAYAAKHRAAGNGFGFGLVDRDSVARTLSARYYKDGSEILV
                     SQDQGKNPRRLTPRECARLMGFPDSFRISVSDTQAYKQFGNSVAVPVIATVAQAMRPW
                     ILNSRQSEESRPANARGAFSQEPVAVSP"
     gene            2503..2916
                     /locus_tag="VHE33_04675"
     CDS             2503..2916
                     /locus_tag="VHE33_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HVT96778.1"
                     /translation="MTDVHTREQRSRNMAAIRSRNTKPEVRVRRVLHGMGFRFRLHRK
                     DLPGRPDIVLPKFRTAIFVHGCFWHCHRCKYGSVVPATRAEFWAAKRGGNVARDRRHA
                     AALRRLGWRVVVLWECEVRSDEAAEAKVRSLRIGR"
     gene            2906..3841
                     /gene="murQ"
                     /locus_tag="VHE33_04680"
     CDS             2906..3841
                     /gene="murQ"
                     /locus_tag="VHE33_04680"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF009222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HVT96779.1"
                     /translation="MAAEIASNRMPEPNTELANLPTEARNPASEHLDEVSTLEMVGIL
                     HSADQEAVAAVGRVLPQIAAAVDAIATRMENGGRLFYLGAGTSGRLGVLDASECPPTY
                     NTPPEMVQGIIAGGDVALRRSIERAEDNPELGARDLEEHDFGSGDVLVGIAASGRTPY
                     VLGGMEHARRLGALTVGLTCVAGSPLALAGEIAIVPETGPEVVTGSTRMKAGTATKLV
                     LNMLSTGALVRLGYVYGNLMVNVQPTNVKLVDRAARIIAAITGLGPEEAGQLLAKAEA
                     VKTAVVMQKLGLSRIEAESRLASAHGRLKEALRTK"
     gene            3838..4578
                     /locus_tag="VHE33_04685"
     CDS             3838..4578
                     /locus_tag="VHE33_04685"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HVT96780.1"
                     /translation="MKATQFEFRFRLIIILLLYTVGFWAPWTWTAGHYRPPASTAWLA
                     IPTLLDRWGWMRLDHASLLVTWLAVVLGLAGAALRLWGTAYLSASVVHSSAMHGDQVM
                     ASGPYRYVRNPLYVGSWLLGLSIAILMPPSGAAVFAVLLAVFYFRLILGEEAFLEGQS
                     REGYLRYKSHVPRLLPRLGARIPASSAHPQWGTSLVAEIFPVVFSICLAALAWRYQPF
                     LLVQCLLVSFGLSLVTRAFLPKKPVAAA"
     gene            complement(4600..4743)
                     /locus_tag="VHE33_04690"
     CDS             complement(4600..4743)
                     /locus_tag="VHE33_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96781.1"
                     /translation="MAGTPNTVGLILLFTVIFLACILWATHGNTATSDPEEEDVGMPG
                     KTS"
     gene            complement(4855..4992)
                     /locus_tag="VHE33_04695"
     CDS             complement(4855..4992)
                     /locus_tag="VHE33_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96782.1"
                     /translation="MDVTPSTIFVVLMLPLIFAVCILWATHGHPSNADNEDLSVVAKD
                     H"
     gene            complement(5169..>5789)
                     /locus_tag="VHE33_04700"
     CDS             complement(5169..>5789)
                     /locus_tag="VHE33_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HVT96783.1"
                     /translation="GVPVCSNLSAGLRKRGIDVRLSVFNDADAVAAGIAATRRQMDRQ
                     VRVWTIGNGIGFGRYPYTEGPWEGGHMVVTLDPKENYCGCGGRGHLEGIMGHRAMRLR
                     FLDMEPDEVFPAAKRGDKRCTEFVELWHRALAAATASQIHLEGPGRFYFTGRDIQRLE
                     LPRLKEHLWEMVKMSPLQNYVVEVLPEDPALAVIGAGVAALLESKC"
BASE COUNT         1062 a         1666 c         1975 g         1086 t
ORIGIN      
        1 aggcaaagtc aattcaggcg atccgaaagc cgagggcagg ttggcgccga ggccaatggc
       61 agcgagcggg ctcacaacgc cacactacac cagggagaat gcggggtggc cgggcgtctc
      121 gcgctatgct caaatccatg aagatcggct tgtttggcgg aagcttcgat ccgccgcatc
      181 ggggacatgt tgcgctggcg cggctggcga tcgaaagcgt cggcctcgat cgcgtgctgt
      241 ttgcgccggt gggcgtgcag ccgctcaagc gcgatcggcc ccaggccagt tttgccgatc
      301 ggctggagat gacagagctg gcgattgccg gtgaaagggg catggagttg tcgcagatcg
      361 atgcgccgcg cagcgatggc cggccgaact acacaatcga tacggtccgc ttgttgctgg
      421 gtacgctggc tgctgacgac gagctttatt gcctgatggg cgccgattcg tttctgagca
      481 tcggcaagtg gcatcgtgcc gccgatctgc tggtcgcatg caattttgtt gtcgcggcac
      541 ggccagggtt cgatctgggc cggatcacct cggctttgcc ggcgagcatc gcagtcgccg
      601 cgtgcgacgg ggcgggcgcg gagaacctgg tgctgtgcct gcacggcgcc ggagagagcg
      661 aatcgcgcct gtacctgctg ccgcatctgc aggaacgggc ttcggcgtcc gggatccgcc
      721 gatccctgca ggagggcgcg gagccacggt gggatcttgc ccccgcggtc gcggcgtata
      781 tcgctgagca caggctctac cagaacagtc gcgcctcctg aggaggccgg ctgcaaatcc
      841 gcaggctgtt gccgtaccat cgaacgagta cgttccgacc cacgcatgac actgactgaa
      901 acgcgcaaaa tggtgctggc cgcggttgcg gcctgcgacg agaagaaggg cgaagacaca
      961 agagttctgc aactggattc agcagactcg gcattcacgg attttttcct gatcaccagc
     1021 acggcgaatg accggcagtc gcaggccatt gccgacgaaa tcgagctgcg cctgaagcgc
     1081 gacttcggga cgtatcccaa ctccgtcgag gggcggcgcg ccggcgagtg gatcctgatg
     1141 gactacgtcg acttcgtcgt ccacatcttt cttcgcgaca agcgcagctt ttacgacatc
     1201 gagcgcctgc ggaagtcggc gcgcacgatt gagctggccg agctgaccgc agcgctgacg
     1261 gagaagacgc tcgcggcgcg gaagaaagct ccggtcaagc gcgcagcgaa gaaggcggtg
     1321 acgaagaagg ctacggcaaa gaaggctgcg aagaaaagcg caggcaaaaa tataaaaccg
     1381 gcagcgaaat ctgccggaaa gcgcggaaaa tagggtcgtg ccgagggcag cgttcacctt
     1441 catcgacctc tttgccggta tcggcgggat gcgcatgggg tttgaggctg ccggtggccg
     1501 atgcgttttc acgagcgagt ggaatccgtt cgcgcgcaag acctacgcgg cgaatttcgg
     1561 cctgagcgag gcgatcgccg gggacatccg tgagcaggat gaaaacgaaa tccccgacca
     1621 cgacgttctg gtcgcgggat ttccctgtca gcctttttca atcgccggcg tgagcaagaa
     1681 gaatgcactc gggcggccgc acgggtttgc ctgtgcgacg caggggacgc tgttcttcga
     1741 cgtagctcgg atcattgctg ccaaacggcc gcgtgcgttc ctgctggaga acgtgaagaa
     1801 cctggtgaac cacgatcgtg ggcgcacgtt tcgggttatt cgccaaacgc tgaaagaaga
     1861 actgggctat tgcatctcgt ggcgggtgat cgacgcggtg cattttgtgc cgcagcaccg
     1921 cgagcgcatc ctgattgccg gctttcgcga agaatgcaag ttcgacctgg atcaggtgaa
     1981 gctgccagcg gccgctccga aactgggctc cgtgctccat ccggaagatg gcagcgaacg
     2041 cccggaggag ccctacacgc tggcgccgcg tggcggggtc aacgcgaaat acacgctgtc
     2101 ggacaagctg tgggcttacc tgcaggcata cgccgcgaag catcgcgcgg ccggcaatgg
     2161 atttgggttc ggcctggtgg atcgtgattc ggtcgcacgc acgctgtcgg cgcggtacta
     2221 caaggatggt tcggagattt tggtgagcca ggaccagggc aagaatccgc gccgcctgac
     2281 gccgcgcgag tgtgcgcggc tgatggggtt tcctgacagc ttcaggattt cggtttctga
     2341 cacccaggcg tacaagcagt tcggcaacag cgtggcggtg ccagtaattg caaccgtcgc
     2401 gcaggcgatg cggccgtgga ttctcaactc gcggcaaagc gaggaaagtc gcccggcgaa
     2461 cgccagaggc gcattttcgc aggaaccggt tgcggtttcg ccatgaccga cgttcacact
     2521 cgcgagcagc gcagccggaa catggcggcg atccggtcgc gcaatacgaa gcctgaggtt
     2581 cgggtgcggc gcgtcctgca cgggatggga tttcgtttcc ggctacatcg caaggacctg
     2641 cctggacgcc cggatatcgt gctgcccaaa tttcgtacgg cgatctttgt gcatggttgt
     2701 ttctggcatt gtcaccgctg caaatacggg tcggtggtac cggcgacgcg ggcggagttc
     2761 tgggcggcga aacgcggtgg caatgtcgcg cgcgatcgcc ggcatgctgc ggcgctgcgc
     2821 cggctgggct ggagagtcgt ggtgctgtgg gagtgtgagg tccgctccga tgaggcggcc
     2881 gaggcgaagg tgcggagtct gcggattggc cgctgagata gcatcgaatc gaatgccgga
     2941 gccaaacacc gaactcgcaa accttccgac tgaagcccgc aatcctgcct ccgagcacct
     3001 cgacgaggtc tcgaccctcg aaatggtcgg catcctgcac tccgccgatc aggaagcggt
     3061 tgcggctgtg ggccgcgtgc tgccgcagat tgccgccgct gtcgatgcca tcgcaacgcg
     3121 catggagaac ggcgggaggc ttttctacct cggcgccgga acctcgggcc ggctgggcgt
     3181 gctcgatgcc tccgaatgtc cgcccactta caacacgccg ccggagatgg tgcagggcat
     3241 tatcgccggc ggagacgtgg cgctgcggcg ctctatcgaa cgcgccgaag acaatccgga
     3301 actgggcgca cgcgatcttg aggagcatga ttttgggtcg ggcgatgtgc tggtgggcat
     3361 tgccgccagc ggccggacgc cgtacgtcct gggcggtatg gagcacgcgc ggcggctagg
     3421 cgcgctcaca gtgggattga cctgcgtggc gggatcgccg ctggccctgg caggtgagat
     3481 tgcgatcgtg ccggagacgg ggcctgaggt ggtgaccggc tccacgcgga tgaaagcagg
     3541 cacggcgacc aagctggtgc tgaacatgtt gtcgacgggt gcgctggtgc ggcttggcta
     3601 tgtgtacggc aacctgatgg tgaatgtgca gccgacgaat gtgaagctgg tggatcgagc
     3661 ggcgcggatc attgcggcga tcaccggcct gggtccggag gaagcggggc agttgttggc
     3721 gaaagcagag gcggtgaaga cggcagtggt gatgcagaag ctgggattga gccggattga
     3781 ggcggaatcg cgacttgcgt cggcacacgg ccggctaaaa gaggcgctga gaacgaaatg
     3841 aaagccaccc agttcgaatt ccgcttcagg ctcatcatca tccttctgct gtacacggtg
     3901 gggttctggg caccgtggac gtggacggcg ggccattatc gcccaccggc cagcaccgca
     3961 tggctggcca ttcccacgct gctggaccgc tgggggtgga tgcgtctcga ccacgcctca
     4021 ctgctggtca cgtggctggc ggtggtcttg ggattggcgg gggccgctct gcgcttgtgg
     4081 gggacagcgt atctgagcgc ctcagtggtc cactccagcg cgatgcatgg cgatcaggtg
     4141 atggcgtcgg ggccgtatcg gtatgtgcgg aaccccctct acgtgggatc gtggctgctg
     4201 gggctgagca tcgcgattct gatgccgcct tcgggggcgg ccgtttttgc ggtccttctg
     4261 gcggttttct atttccggct cattctggga gaagaggcct tcctggaggg ccagtcgaga
     4321 gagggttatt tgcgctacaa gagccatgta ccgcggctgc tgccgcggtt gggagcgcga
     4381 attccggcgt caagcgctca tccgcaatgg ggaacgagcc tggtggcaga aatcttcccg
     4441 gtggtgttct caatctgcct cgcggctctg gcgtggcggt atcagccttt tctgctggtg
     4501 cagtgcctgc tcgtgagctt cggcctgtcg ctggttacgc gggcattttt gccgaagaaa
     4561 ccggtggccg ctgcttaaag gcggaacgtc agtggctttt caggacgtct tgccgggcat
     4621 accgacgtct tcctcttccg ggtcggacgt agcggtgttg ccatgcgtgg cccacaggat
     4681 gcaggcaagg aagataaccg taaagagcag gatgagacct acggtgttcg gggtaccggc
     4741 catgattgac tcccgttagg aaagagtctc cacaaccggg gtttcgttgt ctcgccggag
     4801 gcagaaattc tctctcggtt acggaaaccg ccccggtacc cgcccgcgaa aagctcagtg
     4861 gtccttcgcc accaccgaaa gatcctcgtt atccgcattc gacggatgac cgtgagtcgc
     4921 ccagagaatg cagacggcaa agatcagggg caacatcaga accacaaaga tcgtgctggg
     4981 agttacatcc atacgcttcc tcccgggctt attgtgcgcc gcccggggat cgcggaggcc
     5041 aatttttgat ctcacgaacg atcggaggta accggggtgc gggcgcgtcc acgggaagcg
     5101 aattctcgcc ggtcatagcc agcaccggat tcatgctgca catcggggaa gaccgttcca
     5161 gagttacgtc agcacttgct ttcgagcagc gccgccacgc ctgcgccgat gaccgcgagt
     5221 gcaggatcct cgggcagcac ctcgaccacg taattctgca gggggctcat cttcaccatc
     5281 tcccagagat gttccttgag ccgggggagt tccaggcgct ggatgtcgcg cccggtaaag
     5341 tagaaccgtc cgggaccctc gaggtgaatc tgcgaagcgg tggccgcggc gagggctcga
     5401 tgccacagct ccacgaactc cgtgcagcgc ttgtcgcccc ttttcgccgc gggaaagact
     5461 tcgtccggtt ccatgtcgag aaagcgaagg cgcatggcgc ggtggcccat gatgccttcg
     5521 agatgaccgc ggcctccgca gccgcaataa ttttctttgg gatcgagcgt cacgaccatg
     5581 tgaccgcctt cccagggacc ttcggtatac ggatagcgtc cgaagccgat gccgttaccg
     5641 atggtccaga cgcgcacctg gcgatccatc tggcggcggg tggctgctat gcccgctgcg
     5701 accgcgtcgg catcattgaa gacgctcagc cgcacatcga tgccgcgctt cctgaggccc
     5761 gcgctgaggt tgctgcacac gggtacgcc
//
LOCUS       DASZYN010000186         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_264247, whole genome shotgun sequence.
ACCESSION   DASZYN010000186 DASZYN010000000
VERSION     DASZYN010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264247"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VHE33_04705"
     CDS             complement(<1..296)
                     /locus_tag="VHE33_04705"
                     /inference="COORDINATES: protein motif:HMM:NF013314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVT96784.1"
                     /translation="MRIRVIGGGLAGPEAALMAARMGAEVDLYEMRPVQTTPAHQTED
                     FGELVCSNSLKSESENTAPWLLKQEMRRAGSPLLACADASAVPAGHALAVDRVE"
     gene            complement(514..1041)
                     /locus_tag="VHE33_04710"
     CDS             complement(514..1041)
                     /locus_tag="VHE33_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT96785.1"
                     /translation="MDTLLIINPMTTIREAGRDDVALILEFIRDLAEYERASGAVQVT
                     EAELLRDGFPEAGAQRYFETVIAEEDGEPAGIALFFPVYSTWRGRAMHLEDLFVRPRF
                     RGRGIGRALLEKVAAIAVERGCVLLFWHVLDWNTPAIEFYKTLGADWMDDWTRMRVKD
                     DALRELAAGAMRGVK"
     gene            1347..>2095
                     /locus_tag="VHE33_04715"
     CDS             1347..>2095
                     /locus_tag="VHE33_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT96786.1"
                     /translation="MQRCFALVVPTVLAAALALPANSSAFREAEQAPQTTSRLAVAQI
                     PPTAPQVTAVNLPPELRGDLAMARQQYLRAIDAYTQVPARTATIWNKLGMAYHHLFAM
                     DEARRDYERALRLQPDYAEALNNLGAIYYAKKNYKKAIRYYRKAIAISPQSATIYSNL
                     GTAWFARGKSEKGIEAYRTAFSLDPEVFASDSALLVNEGLPAHERALQDFCLAKLFAA
                     SGKKEEAIEFLRKALNEGFDDRKRILADQTLA"
BASE COUNT          433 a          785 c          513 g          364 t
ORIGIN      
        1 aactccaccc gatccacggc cagcgcatga ccggccggaa ccgcgctcgc atcggcacag
       61 gcgagaagag gcgatcccgc ccgccgcatc tcctgcttca gcagccacgg cgccgtgttc
      121 tcgctctcgc ttttcagcga attgctgcac accagctccc caaaatcttc cgtctgatgc
      181 gcaggcgtcg tctgcaccgg gcgcatctcg tacagatcca cctccgcgcc catccgcgcc
      241 gccatcagcg cagcctcagg acccgccagt cccccgccaa tgactcgaat cctcattctt
      301 ctccaaaaaa cgtacgcagc ttgaaacgcc aaacacaggc cgttgctccc atgatcgcga
      361 gcagcgtcga ttgcgaccgt ttgtaacagg gcacgagttt acgcgtgccg aaaaccacgg
      421 aaatgaattg ggctttagcc cctgccgttc gagagggcac gccgcccaac aagccaatgc
      481 aacggacttc agcccccgag gcacgtcttt gccttatttc actcccctca tcgctcccgc
      541 cgccagctcc cgcagcgcat catccttcac tcgcatccgc gtccagtcat ccatccagtc
      601 cgcgcccagc gtcttgtaaa actcaatcgc cggcgtattc cagtccagca cgtgccagaa
      661 caaaagaacg cacccacgct ccaccgcaat cgccgccacc ttttccagca gcgctcgccc
      721 gatcccacgc ccccgaaaac gcggccgcac aaacaagtcc tccagatgca tggcacgccc
      781 ccgccacgtc gagtacaccg gaaagaacag cgcaatgccg gccggctctc catcttcttc
      841 cgcgatcacg gtctcgaaat acctctgtgc gccagcctcc ggaaacccat cccgcagcag
      901 ctccgcctcc gtcacctgga ccgcacccga agcgcgctca tactccgcca gatcccgaat
      961 gaactccaga atcagcgcga catcgtcccg cccggcctcc cgaatcgtcg tcatggggtt
     1021 gattataaga agcgtgtcca taccactttt cccgagtcat gccgtaccac tggacggcac
     1081 accggattgg ggtggttatg cgcatgcccg tcccgcacgc aacacaacat caccactgca
     1141 taattcggtc gtgctggact tcctttttcc ggcttttggc gtcatgatta gggtcaatcc
     1201 agccccaggc tggaccgttc ccccacatgc cccggcaggg atcgcgccga ggtcaccaac
     1261 ggcatcaagc cgcgcgaagg acggttcagg agaccggggc ccccgacgca cccgcttcgg
     1321 gtgcggtggg gtggagggag gcgcttatgc agcgctgttt cgctctcgtg gtcccgaccg
     1381 ttctggccgc tgccttggcc ctgcccgcaa actcttccgc gtttcgcgag gccgaacaag
     1441 ctccccaaac cacctccagg ctggctgtcg cgcagattcc acccacggcc ccccaggtca
     1501 ccgccgtcaa cctgcccccg gaactccgcg gcgatctggc catggctcgc cagcagtatc
     1561 tgcgcgccat cgatgcttac acgcaggtgc ccgccagaac cgccactatc tggaacaagc
     1621 tcggcatggc ctaccatcac ctctttgcga tggatgaggc ccgccgagat tatgagcgcg
     1681 cgctccgcct gcaaccggat tacgccgaag ccctcaacaa cctcggcgcc atttattacg
     1741 caaaaaagaa ctacaaaaag gcgatccggt attaccgcaa agccatagcg atcagtccgc
     1801 agtctgcaac catctacagc aacctcggca ccgcctggtt tgcccgcgga aaatcggaaa
     1861 aaggcattga ggcctaccgc accgccttct cccttgatcc ggaagtcttc gcgtccgatt
     1921 cagccctgct cgtcaacgaa ggcctgccag cgcacgaacg cgccctgcag gacttctgcc
     1981 tcgccaaact ctttgccgct tcgggcaaaa aggaggaggc gatcgaattc ctccgcaagg
     2041 ctctcaacga aggcttcgac gaccgcaaaa ggatcctcgc cgaccaaacc ctggc
//
LOCUS       DASZYN010000187        17880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_775301, whole genome shotgun sequence.
ACCESSION   DASZYN010000187 DASZYN010000000
VERSION     DASZYN010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 17880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17880
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775301"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VHE33_04720"
     CDS             <1..820
                     /locus_tag="VHE33_04720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT96787.1"
                     /translation="CPSCSHVWLFDPPPPEQMMQFYDPTYHQAVTRSGEGDAVRWGRL
                     LKAIGQYKTRGAVLDIGCSSGGFLTHLKDRGWELAGIELNPEAAERARQNTGGQIMTG
                     DIDSVEFPPGSFDVITCYDVLEHLYEPRMVFQKVARWLKPGGIFYIFVPNVLSWEARM
                     FQSFWYPLDLPRHLHFFSPASLTALSGSAGLRIVHVTTPPGNYLEHNASRLATWLLRR
                     TTKSTLAIDFSGPVDPVSRLIRKGMRLTVESGFGKLASLCHAAPSLQAVFQKPD"
     gene            complement(887..1351)
                     /locus_tag="VHE33_04725"
     CDS             complement(887..1351)
                     /locus_tag="VHE33_04725"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HVT96788.1"
                     /translation="MTSTLQPRSISSDRIIVKEIETETLLYDERTHRAWCLNQSSACI
                     WRLCDGRRTVQQIAEAAGSELGSYVNEDLVLLALTELREKELLEEPSAALLPDDISRR
                     AMIGRAGLAAAALLPVIAAITAPTAAAQLGGSAGTGGDGGGMSSHTDPARVK"
     gene            complement(1348..2529)
                     /locus_tag="VHE33_04730"
     CDS             complement(1348..2529)
                     /locus_tag="VHE33_04730"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HVT96789.1"
                     /translation="MRPGMQLLLALLRGEARAEDGSETEWETVLRLAEREKILPWTAA
                     QLRLISGAIAYGIDEQLRKIRRDAQMAAFFWSSTLRSILAEFHDREIAVVSLKGPWMA
                     ERLLGDAALRSYSDLDLLVRPEDVARSEELLFELGFRPRRCRDDHERPWYRDAVSVDL
                     HHDVEHPLAWDFDVTRAWDRTCITPFQGVPVRLLAPADELVFLCLHSTRHRFERLGHI
                     LDLTFAFQKLPLPWAGSYLVPNPEMARLVTFASMMAARLDPRCRARRSADLSGRDHER
                     LEQLADQVWHERTTESCPVTDWRAQHEFYLRMETRPLRRLLRRVRDVRILLTRLMEDD
                     FVFAARFHMRRPWQVWLLRPIRLLFHASRAARSRSRPQQGGIGNYRSPEAVSFTGRTT
                     G"
     gene            complement(2526..3380)
                     /locus_tag="VHE33_04735"
     CDS             complement(2526..3380)
                     /locus_tag="VHE33_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96790.1"
                     /translation="MPAADVATHDRSQPSQGCADHVLNMISYGVPFRLSAESPEILER
                     MRRQAPWGSATQPMAALGARNFALRAVQSDARYRVLADGKVVSDDQAVVPALSQLSGH
                     IMVYVAEHAPDLVFVHAGVVAWQDRALLLPGVSHAGKSTLVAELVRAGATYYSDEFAL
                     LDPEGRVHPFARELGMRRPGNSNQTMVPVTQLRGCAGTKALTVAMIVFTNYAENACWA
                     PEPMSHGRAVLEMLLHTAPVQRTPARVLGTVSATVENAAVWKSPRGEAPPAARALLAA
                     LATGGAPA"
     gene            complement(3412..5268)
                     /locus_tag="VHE33_04740"
     CDS             complement(3412..5268)
                     /locus_tag="VHE33_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HVT96791.1"
                     /translation="MRPVPRQSGTALQQSLLLVGAFSLVALVIGCGGSSGTSNSGSGS
                     GSNPPPATPAFAGQMRSGTSPVEGSAISFYSAGASGTGAGATNLVSSQAVMTDASGNF
                     SIPSFTCPSSSAQVYLVGRGGKTSSSGSENSALVMMAALGDCGSLSTSTTVVMNEVTT
                     AAAVWALNPFLGPNAAVGASATNATGLRNAFLTVANLADNSKGTTPGAALPANSSIEA
                     AKLNTLADILWRCDQGTGTACQSLFSAATVNGGTPSNTLDAALSIVRNPGLNVGVVFA
                     LLSQGPYQPVLTAAPHDWLLSATFGKCSSGCGGLDQPGSLAIDSKGNVWVANYFGGAA
                     SEFSPTGVPAAPDGFAASGLQGSFGITVDGQDSVWIANALGNTTGSVTHLSSIGVNLS
                     GAGYTGGGIYLPYALAATPGGNVWVADYANAAATLLAKDGTPLSGAQGYAASSLIFTA
                     AVAVDANQNGWFAYQGGVAMVTPAGVVTNYSCCEVPAGIALDQGGNIWIADFSASAVI
                     KLSPSGALLGEADGTGGIVTPDGIAVDGSGHVWTANYHGGTISEFTGDTLQPVSSAAG
                     LGLDADLSGPFGAGVDASGNLWISNAFANTLTEFVGAAAPVRTPLLGLPAQP"
     gene            complement(5290..6660)
                     /locus_tag="VHE33_04745"
     CDS             complement(5290..6660)
                     /locus_tag="VHE33_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96792.1"
                     /translation="MNPSRRGSAGIIVAAMAIGLAGCGGSSSSFQTKTPQPAVKALGE
                     VFGGQQPVSSMTIQMFDAGTGGYGSAATALLSTATDSNGKFNFASNPSCASDPNNQDQ
                     IYLVGTGGDPVVAGNGTNSHLALMLALGDCGRLNGSNASPITHVHINELTTVAAVWAL
                     APFMSGNTQAWQHVGTSSTNTAGLRMAFQAANQVVNIDTGTLPGTLPAGATLGQAQVN
                     TIADALEFCINSIDGSGNSPSASCSSLFTNAPSSSGNPGDTITAAMNIAQNPGRNVAT
                     LDQGVSNTSTFQPIVASPTAFTIAIQYTGGGLNAPTAIAADGAGQVWVANSGSSAVSL
                     FDNLGNSKLGTTGTALGGTPQGIAIDLSGNAWVTASDDSVYELSSSGTINPATPFTGF
                     NGPAGIAVDPAGYIWVVNNNDSTVSAFNSTGAAMSGSPFSGAGISSPVGIAINGNANA
                     NCADCH"
     gene            complement(6657..7835)
                     /locus_tag="VHE33_04750"
     CDS             complement(6657..7835)
                     /locus_tag="VHE33_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HVT96793.1"
                     /translation="MTYTATPDPGWTFAGWTYDLTGTSNPASLTAKDESLVWANFNVT
                     NVPLAITSLSPPSVAAGSSTFTLTINGTGFASNSVVIINGSTFPAVTFVNSTELQVQV
                     DSSLVSSAGTFDVSVENFPVGSSGCAVFAYDTFAVTASGSGLITPTINWTPAAEIVRG
                     DAGTKVLNATTTPPDIGGFTYSTSPGGDVTGGTSGLAPGTYTLTATFHPTDPQYTSTT
                     ASVTVKVAGETVWIVNGGGSLSELTGDGTAVSSSAYAGENGAAAIDSGGNVWSVGSGA
                     MPLVETSQVGVVQNAISSGGGLNTPIGIAIDGASQIWVTNFNDSVSLFTNAGAALSPS
                     GGFTDPSLSAPSGVAVDLTGSVWIANNGNNSVTRILGAAAPAAPLATAAQNNTTGAKP
                     "
     gene            complement(7851..9617)
                     /locus_tag="VHE33_04755"
     CDS             complement(7851..9617)
                     /locus_tag="VHE33_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HVT96794.1"
                     /translation="MFSLKASLPRPVQFAVPTLLLLAFTAASRAQSRPGPELAHLRPQ
                     PQSVGVADPGSTWPSGVIYYDDGGCIAGNTCPNLAQAISTFNADFPGVVQWTERTSQT
                     TYVVITLSGTGGRGDVDTIGYPAFAGALNLNCNTDCSVATLLHEMGHIIGLYHEHTRT
                     DRDSYVTLNYNNVIKGSWTGNFAINTQNQQLLSPYDYASVMQYPSYVDSRNGGPVIET
                     IPAGIPLQGTEGVPGAGNEDYSAGDKEAIRRLYGAAPTQVTVTSNPVGLQVLVDSVPC
                     TTPCTESWTVGSTHNLSVADGVQTLTGNIENSGQSATFYYTYGRWSDSTQQTHSITVQ
                     GGNGSPAFPTGSPAIATYSANFVQLVPYTSITTGSGIVDASPEPQTYTGDASDVFFVA
                     REQVTLTASALPFGGWVFYEFNAQAPYFWLPGGLSENPKTFFVPDTGNPVAVNAEFTT
                     FPIYTVDVEPSSPIQNQFSSNLYAYVDGNFWTTPKNFSPDPALDGSAWDEGTTHTLSL
                     SFNGDSTNPPEQPYSANTEFAFSSWSDGGAYSHTTAGLSPDGATYTATVTPLYQPATN
                     FSSLPPCGGTAAITPASSNGGF"
     gene            9955..11520
                     /locus_tag="VHE33_04760"
     CDS             9955..11520
                     /locus_tag="VHE33_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HVT96795.1"
                     /translation="MLFTARSAAVYGIDASIIDVEVDFSKFQSEKEIFNTVGLPDAAV
                     RESRDRVRAAIRNSGFDLPPTHITINLAPADIKKEGSGFDLPMAVGILGAYGALHQQN
                     GLPDFLLIGELGLDGALRPVPGMLPVAVAARERGIRNLVIPKANAREAAVVDGVNVYP
                     VDSLLQVRDLLNQASNGGIQTPAFHVDAAGVLGELQHFSLDFADVRGQQAAKRALEVA
                     AAGGHNILMIGPPGSGKTMLARRLPSILAPLTFDEALQTTKIHSVAGVLDAEAGLVTQ
                     RPFRAPHHTISDAGLIGGGVIPRPGEVSLAHNGVLFLDELPEFPRNVLEVMRQPLEDR
                     HVTIARASMSLTFPASFMLAAAMNPCPCGYFNDKSRDCHCTPPMIQRYVAKISGPLMD
                     RIDIHIEVPAVQYRELRGAAAIENSATIRTRVLGARERQRQRFAAAKEKIFSNAQMGT
                     RQIRAHCELNPEAEKLLERAMLQQGLTARAHDRILKVARTIADLEGTASLAVSHIAEA
                     IQYRTLDRSYWAG"
     gene            11614..12618
                     /locus_tag="VHE33_04765"
     CDS             11614..12618
                     /locus_tag="VHE33_04765"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HVT96796.1"
                     /translation="MNLAPIVAAPADEERQASVPLNIDALQPLTVQPERWMLGSMVGR
                     APVMQHLFQRMRATAPHFRLVNVEGEQGTGKLQVAQTLHRIGAAASGPFVPAIALEFI
                     EAPDSIWQEAQGGLLYLARVDELTTDQQRRLRDFLERAAHERMRAAKNLAPLQIVAGS
                     TQSLRRLAGSGAFRSDLASHLTAVRFGIPPLRDRKDDIPLLAALFLRDWSREHNNPLR
                     GFAAGVLPRLTSYAWPGNVRELESVVAAAALECSGQWVRPIDIPPLHSPAPSMPAPMP
                     LDPLADDPNLDRAILRHITRILARANGNKVRAARMLGISRSTLYRLLDPHLPADSTTE
                     "
     gene            12789..14357
                     /locus_tag="VHE33_04770"
     CDS             12789..14357
                     /locus_tag="VHE33_04770"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HVT96797.1"
                     /translation="MTPKSATPGLRRTATVPDSSPSSDGQLKPDRLKTGIDGLDDILH
                     GGFPAGHLYLIEGDPGTGKTTLALQFLLEGIRRGERVLYITLSESATELRQVGRSHGW
                     SLETLEIAEMIPPDDDLRPEAQYTVFHPSEVELADTIASILKKIDEIQPQRLVFDSLA
                     ELRMLARDPLKYRRQILALKRHLSGGNITVLMLDDRTSNSNDDLQLQSIAHGVIVMQG
                     LGRDYGIRRRRLEIHKLRGSAFREGYHDYVIRTGGLDIFPRLIAAEHKPDFRRSPVAS
                     GLPHLDDLLRGGIDTGTSTLLTGPAGCGKSTVAFHYAYAAAMRGEKAMIFTFDESRAT
                     LTDRARGLGMDPEPVLQSGRLQVQQVDPAELSPGEFVSRIRRLVDDGHLSVLVIDSMN
                     GLLNAMPDEQYLAMQFHELFSYLGQQGIATIITLAQAGVIGTAMSAPVDVSYLADTIL
                     LFRYFERAGEIRQAISVVKKRSGSHERTIRELFMEPGRVSVGNPLSEFEGVLTGVPRL
                     IAKRDPAQEGRSVS"
     gene            14542..15543
                     /locus_tag="VHE33_04775"
     CDS             14542..15543
                     /locus_tag="VHE33_04775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT96798.1"
                     /translation="MHGAIHKVREVIEHQPTWSDFPLILLTVGGRVTAESERLREFRM
                     PLGNLFLLERPIRPETLLSTLEIALRGRQRQYQIRDQMHQYARAQDALRRSEKLAVTG
                     RLAASIAHEINNPLEAVTNLLYLMRASENPAQAHTWLAQADQELARVAEITRQTLRFY
                     REPSHPVDTVVASVLRSVLVLYNSRIGAANVEILLEVEAAEATVLASPGELRQVIANI
                     VGNAIDAMRHGGRLRLRLSEENDASESPRVRLTIADTGSGIPTALLPTIFEPFVTTKG
                     ETGTGLGLWVTGEIIRRNGWDIRVRSRCSPHRSGTVFSITMPLRSAVAQTESALAVA"
     gene            15688..16158
                     /locus_tag="VHE33_04780"
     CDS             15688..16158
                     /locus_tag="VHE33_04780"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HVT96799.1"
                     /translation="MKKYSFTATIEAASVGGGGAYILFPYDVEKEFGTRGRVPVQATF
                     DGVPYTGSLVKYGQPQHMLGILKSVRDQLGKQPGDKVKVVLWKDEAPRTIETPADFAA
                     LLTKEKLLAAFEKLSYTHRKEYVRWITEAKKEETRTARLTKSIAMLRKGVKTPD"
     gene            16291..>17880
                     /locus_tag="VHE33_04785"
     CDS             16291..>17880
                     /locus_tag="VHE33_04785"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HVT96800.1"
                     /translation="MKLRIVASLLLAYSAAAVVPQLPAQNPPAAQTAGDAAARGKALN
                     DLFAQIWEDRLSHSPEFASTIGDDRFNDQLTDYSIEAYNQRLARGRDYILRLSAIDTN
                     GLPDQEVLSRDLMIRDLTDDQEESAFKPWEMPVNQFNGLHLSLAQLVPRLRFNSARDY
                     DDYIARLGKIPAAFRQISDNMLTGVDDRRVPPKYLLEKVLTQVNALLAQKPEDSPFAL
                     PLKKFPASISAADQARYREAILTAIAKQVDPAYQRFAKFLQGVYIPAGRSDPGVWALP
                     DGDAYYAFRVKQSTTTDLTPAQIHQLGVDQVAQDEAAMLVIAKKLGYPTIAAMRTAIA
                     ANPKLHPASREQLLDAYRADLDKMRPKLPDLFGRLPKAPLRVEAVPAFMEKDQAPAYY
                     EQGTPDGSRPGTVFVNTYDYEHHSLANVESVAYHEGLPGHHLQISIAQELTGLPTFRK
                     YLHYTAFTEGWGLYAEQLGKDVGFYQDPYSDFGRLEGDIFRAIRLVVDTGVHSQHWTR
                     QQMVDYFHAHSGLDDATINAEC"
BASE COUNT         3401 a         6227 c         4863 g         3389 t
ORIGIN      
        1 ttgcccttca tgttcgcacg tgtggctgtt cgatccgccc cctcccgagc agatgatgca
       61 gttttacgat cccacctatc atcaggccgt cacgcgctcc ggcgagggcg acgcagttcg
      121 ctgggggcgg ctgctcaaag cgatcggaca atacaaaacc agaggcgcgg ttctcgatat
      181 cggctgcagt tcgggtggat ttctaactca tctcaaagat cgcggctggg agttagccgg
      241 cattgagcta aatcccgaag cagcggagcg agcccgccag aacaccggcg gccagatcat
      301 gacgggcgat atcgacagcg tagagtttcc gccgggtagc tttgacgtca tcacctgcta
      361 cgacgtgctc gaacacctct acgaaccgcg gatggtcttt cagaaagtcg cgcgctggct
      421 caagcctggc ggcatcttct acatctttgt tcccaatgtt ctctcgtggg aagcgcgcat
      481 gtttcaatcc ttctggtacc cgctggacct cccgcgccat cttcatttct tttctcccgc
      541 atcgctgacc gcgttgtcag gctccgcggg actgcgcatc gtccacgtca caacccctcc
      601 cggaaactat ctcgaacaca atgcgtcgcg actggcgacc tggctcctcc ggagaactac
      661 gaaatccacc ctcgcgattg atttcagcgg tcccgtggat cctgtttcac gtttgattcg
      721 caagggcatg cgtctaaccg tcgaaagcgg cttcggcaaa ttggcatcgc tctgccatgc
      781 ggccccaagc ctgcaggcgg tcttccagaa gccggattga tttctacagc agtgccggac
      841 gccgctcccg gacgcatgcc ccgagtatcg gcatccggac gatccgctat ttcacgcgcg
      901 ctgggtcggt gtggctgctc ataccgccac cgtcgccgcc cgtcccggca gaaccaccga
      961 gctgtgccgc cgcagtaggt gctgtgatgg ccgcgatcac cggcaggagc gctgccgccg
     1021 cgagccccgc tcggccgatc atcgctcgac gcgagatatc gtcgggcaac agggcagcag
     1081 agggctcttc cagaagttct ttttcgcgca gttcagtaag ggccagcaga acgagatctt
     1141 cgttcacgta actgcccaac tcgctgcctg ccgcttccgc gatctgctgc accgtgcgtc
     1201 ttccgtcgca cagccgccag atgcaggccg aactctggtt caggcaccag gcgcgatgcg
     1261 tcctctcgtc atacaggagc gtttccgttt caatttcctt cacgatgatc cgatcgctgg
     1321 aaatgctgcg aggctgcaaa gtcgatgtca tcctgttgtc cttcctgtga agctgactgc
     1381 ttctggggat cgataatttc caattccacc ttgctgcgga cgcgaccgcg aacgcgctgc
     1441 tcgactggcg tggaacagaa gccgaatggg ccgcagcaac cacacttgcc acggccggcg
     1501 catgtggaag cgcgcggcga aaacaaaatc gtcttccatc agccgtgtaa gcagaatccg
     1561 cacatcgcga acccggcgga gaagccgtcg cagcggacgt gtctccatcc tcaagtagaa
     1621 ctcgtgttgc gcgcgccaat cggtcaccgg gcacgactct gtcgtacgct cgtgccaaac
     1681 ttgatcggca agctgctcca gccgctcatg atcgcgcccg gaaaggtcgg cagaccgccg
     1741 tgctctgcaa cgaggatcga gtcgcgcggc catcatcgag gcaaacgtca caagccgggc
     1801 catttcgggg ttcggcacca aatagcttcc ggcccatggc agcgggagct tttgaaaagc
     1861 aaaggtcaga tcgaggatgt gaccgagccg ttcgaagcgg tggcgcgtgc tgtgcaggca
     1921 aagaaaaacg agttcgtcgg cgggcgcgag gagccgcacc ggtacaccct gaaacggtgt
     1981 gatgcaggtc cgatcccatg cgcgcgtcac atcgaaatcc cacgccagag gatgttcgac
     2041 gtcgtgatgc agatccaccg agaccgcatc ccgataccac ggccgttcgt gatcgtcgcg
     2101 gcagcgcctc ggccgaaaac caagttcgaa cagtagctct tctgatcgcg ccacatcctc
     2161 aggccggacc agaaggtcga ggtcggagta cgatcgcagg gctgcgtcgc ccaacagccg
     2221 ctccgccatc catgggccct tcagggagac gaccgcgatc tcgcgatcat gaaactccgc
     2281 aaggatgctc ctgagggttg aactccagaa aaacgcagcc atttgtgcgt cgcgccggat
     2341 ctttcgcaat tgctcgtcaa tgccgtaggc gatcgcgcct gaaatcaacc gcagctgcgc
     2401 cgcagtccag ggcaaaatct tttcccgttc tgcaagacgc agaacagtct cccactccgt
     2461 ttccgagcca tcctcggccc gcgcctctcc gcgcagcaga gccagcagca gttgcattcc
     2521 cggtctcatg ccggcgctcc tcccgtggcc agggctgcga gcaatgcgcg cgccgccgga
     2581 ggtgcttcgc cgcgcggcga cttccagaca gccgcatttt caaccgttgc ggaaaccgtc
     2641 cccagcaccc gcgccggagt tcgctgcaca ggcgcagtgt gcagaagcat ttccagcacc
     2701 gctcgcccgt gagacatcgg ctccggagcc cagcaggcgt tttctgcgta gtttgtaaag
     2761 acaatcatcg cgaccgtcag ggcctttgtc ccggcgcagc ccctgagttg cgttaccgga
     2821 accatcgtct gattcgaatt gcccggccgc cgcatgccca gctcgcgcgc aaatggatgc
     2881 acccggcctt cgggatccaa cagtgcgaat tcatccgagt agtacgtagc tccggctcga
     2941 accagttcag ccaccagcgt ggatttgccg gcatggctca ccccaggcag aagcagcgcg
     3001 cgatcctgcc atgcaaccac gcccgcatga acgaagacca ggtccggcgc atgctcggcg
     3061 acataaacca tgatgtgtcc acttagctgt gagagagccg gaaccacggc ctggtcgtcg
     3121 gagaccactt ttccgtcggc cagcacccga tacctcgcgt ccgactgaac cgcgcgcagg
     3181 gcaaagttgc gtgcacccaa cgcggccatc ggctgcgtag ccgatcccca gggcgcctgc
     3241 cgccgcattc gctcgagtat ttccgggctc tcggctgaaa gccgaaaggg aacaccatac
     3301 gagatcatgt tcagcacatg atcggcgcat ccttgcgaag gctgactccg atcgtgagtc
     3361 gcaacatcgg ccgccggcat gttctcttcc ctcgcgtagg ctgacataac tctatggctg
     3421 tgccggaagc ccaagcagcg gtgtccttac cggtgccgcg gctcccacaa attccgtcaa
     3481 tgtgttagcg aacgcattgc tgatccaaag attgccgctc gcgtcgactc ctgccccgaa
     3541 cggtccgctc aggtcggcat cgagtcccag gcctgctgcc gaagaaacag gctgcagtgt
     3601 gtcgcctgta aactcgctga tcgtgccgcc atgatagttc gccgtccaca cgtggccgct
     3661 gccatcgacg gcaattccat ctggcgttac gataccgccc gttccatcag cctctcccag
     3721 cagcgcgccc gatggcgaaa gcttgatcac cgccgatgca ctgaagtcgg caatccagat
     3781 gttgcccccc tgatccagcg cgattcccgc cggcacctcg cagcaggagt agttggtcac
     3841 cacgcccgcc ggcgtcacca tggccacgcc accctgatag gcgaaccagc cattctgatt
     3901 ggcgtcgact gcgaccgcag ccgtgaagat caaagacgat gcagcgtagc cctgagcgcc
     3961 ggacagcgga gtgccatctt tcgccagcag cgtcgccgca gcattcgcat agtccgcaac
     4021 ccacacattg ccgcccggag tagccgccag cgcgtaagga agataaatgc cgccgccggt
     4081 gtaacccgcg ccggaaagat taacgccgat gctgcttaaa tgcgtgacgc tccctgtagt
     4141 gttgccgagt gcatttgcaa tccagaccga gtcctgtcca tctacggtga tcccgaacga
     4201 tccctggagt ccgcttgcgg caaatccatc cggtgccgcc ggcacgcccg tcggtgaaaa
     4261 ctccgaagcc gctccaccaa aataattcgc aacccacaca tttcctttgg agtcgatggc
     4321 gagggaaccg ggctggtcca gtccaccgca tccggaactg cacttgccaa aggtagccga
     4381 cagcagccag tcatgcggcg ccgccgtcag cacaggctga tagggccctt gcgagagcag
     4441 cgcaaacacc acgccgacgt tcagcccagg attgcgaacg atgctcagcg ctgcatcgag
     4501 cgtgttggaa ggagtaccgc cgttcaccgt ggcagcgctg aacagagact ggcaggcggt
     4561 gccggttccc tgatcgcagc gccacagaat atccgccagc gtgttcagct tcgccgcctc
     4621 gatgctcgag ttggccggca gcgccgcgcc gggagtcgtc cccttactgt tgtccgcaag
     4681 gttcgcaacg gtgagaaaag cattccgcag cccggttgca ttggtcgcgc tggcgccgac
     4741 ggccgcattc ggcccgagga acggattcag cgcccacacc gcagccgccg tcgtcacctc
     4801 gttcatcacc accgtcgtcg acgtgctcag actgccgcag tcgccgagcg ctgccatcat
     4861 taccagtgcg gaattctccg agccggacga cgatgtcttg ccgccgcgcc ccacaagata
     4921 gacctgcgcg ctgctgctcg ggcacgtgaa cgacggaatg gagaaattgc ccgaagcatc
     4981 ggtcatgacg gcctgcgaac tcaccagatt ggtcgctccc gcgcccgtgc cggacgcgcc
     5041 ggcagagtag aacgagatcg cggagccctc aacgggactg gttccgctac gcatctgccc
     5101 cgcaaaggcc ggcgtcgccg gtggaggatt cgagcccgaa cccgaacctg aattcgaggt
     5161 tccgctgctg cctccgcagc caatcaccag cgcgaccagc gagaaagcgc ccaccagcag
     5221 caagctctgc tggagcgctg tgccggattg gcgcggaacc ggtcgcatcg aggtctctcg
     5281 tcatcggttt tagtggcagt cggcgcaatt ggcgttggcg ttgccgttga tcgcgatgcc
     5341 aaccggcgat gaaatgcccg ctcccgagaa tggggatccg ctcatggccg cgcccgtgct
     5401 gttgaacgcg ctcaccgtac tgtcgttgtt gttcacgacc cagatgtatc cggcgggatc
     5461 gaccgcgatt ccggcaggcc cattgaaacc cgtaaacggc gtagcgggat tgatggtccc
     5521 gctggaactc agttcataga cactgtcgtc cgaagcggtc acccacgcat tcccgctgag
     5581 atcaatcgcg atcccctgag gagttccgcc gagcgccgtg cccgtcgttc ccagcttcga
     5641 atttcccagg ttatcgaaca gcgacacagc actactaccg ctgttagcca cccagacctg
     5701 cccggctcca tccgccgcaa tcgcggttgg cgcattcaac ccgccccccg tgtactggat
     5761 cgcaatcgtg aaagccgtgg gactggcaac gatgggttga aacgtcgatg tgttggagac
     5821 tccctggtcc agtgtcgcca cattccggcc cggattctgc gcaatgttca tcgctgccgt
     5881 aatggtgtct cccggattgc ccgagctcga aggcgcattt gtaaaaagac tgctgcagct
     5941 ggcactcgga ctattcccag agccgtcgat cgaattgatg cagaattcca gcgcgtccgc
     6001 gatcgtgttc acctgagcct gcccgagcgt cgcgcctgcc ggcaaggtgc ccggcagcgt
     6061 tcctgtgtcg atgttcacca cctgattcgc cgcctgaaat gccattcgca atcccgccgt
     6121 attcgtcgac gatgtgccaa cgtgctgcca ggcctgcgta ttcccgctca tgaatggagc
     6181 cagcgcccac accgccgcca ccgtggtcag ctcgttgatg tggacatgcg taatcgggct
     6241 cgcattgctg ccgttcagcc tgccgcagtc tcccagcgcc agcatcaacg ccaaatgcga
     6301 attagtgccg tttccggcga caaccgggtc cccgcccgtt cccaccaggt aaatctgatc
     6361 ctggttgttc gggtcgctgg cgcaactggg gttcgaggca aagttgaatt tcccattcga
     6421 atccgtcgcc gtcgacagca gcgccgtcgc ggctgaaccg taccccccgg tgccggcgtc
     6481 gaacatttgg atcgtcatcg aactcaccgg ctgctggccg ccaaaaacct cgcccagcgc
     6541 cttgacggct ggctgcggag ttttggtctg gaacgaggaa ctgcttcccc cgcagccggc
     6601 caacccgatt gccatcgctg caacaatgat gcctgcgctg cctctccgtg atgggttcat
     6661 ggcttcgctc ccgtcgtgtt gttctgcgct gcggtggcca gcggtgccgc cggtgccgct
     6721 gctcccagaa tcctcgtcac gctgttgttc ccgttgttgg cgatccacac gctgcccgtc
     6781 agatcgacag cgacaccact cggcgccgat agcgatggat cggtgaatcc ccccgaaggc
     6841 gacaacgccg cacccgcatt cgtaaacagg ctcacgctgt cgttgaaatt cgtcacccaa
     6901 atctggctcg cgccgtcgat ggcaatcccg atcggcgtat tcaggccgcc gccgctggaa
     6961 atcgcgttct gcaccactcc gacctggctg gtttccacca gcggcatcgc tcccgatccc
     7021 acgctccaca catttccgcc gctgtcgatc gcggccgccc cattctcgcc cgcgtaggcg
     7081 ctggacgaaa ccgcggtgcc gtctccggtc agttcactca ggctgcctcc gccattcacg
     7141 atccacaccg tctcacccgc caccttcaca gtgacgctcg ccgtcgtcga cgtgtattgc
     7201 ggatcggttg gatggaatgt tgccgtcaac gtataggtcc cgggtgccaa gcccgaggtc
     7261 ccgcctgtga catcgccgcc cggcgacgtg ctgtaagtga agccgccaat atccggaggc
     7321 gtcgtcgtcg cgttcagcac cttcgtcccg gcatcgccgc ggacgatctc tgccgcgggt
     7381 gtccagttga tggtaggcgt gatcagcccg ctacccgagg ccgtcaccgc aaacgtgtcg
     7441 taggcaaaga ccgcgcatcc gctcgagcca accgggaaat tctcgacgga aacatcgaac
     7501 gtccccgcag acgaaaccag cgagctgtca acctgtacct gtagctccgt tgagttcaca
     7561 aacgtaacgg caggaaacgt cgatccattg atgatgacca cagaattcga agcgaacccc
     7621 gttccattga tggtcagcgt aaatgtcgaa gaacccgctg ccaccgaggg cggactgaga
     7681 ctcgtgatcg ccagtggcac attcgtgacg ttgaagttcg cccagaccag cgattcgtcc
     7741 tttgccgtca ggctcgcagg attgctcgtt cccgtcagat cgtaggtcca gccggcgaac
     7801 gtccagccgg gatcgggcgt cgccgtgtag gtcaactgcg ttccccacgg ctagaacccg
     7861 ccattgctgg aagctggagt tatcgccgcc gtaccgccac acggcggaag cgagctgaag
     7921 ttggtcgctg gctggtaaag cggagtcacc gtcgccgtgt aggtcgcgcc gtctggcgat
     7981 aatcccgccg tggtgtgcga atacgcccct ccatcgctcc agctcgagaa tgcaaactcc
     8041 gtgttcgcgc tgtacggctg ttccggcgga ttggtcgaat cgccattgaa gctgagcgac
     8101 aacgtatgcg tcgtgccctc gtcccatgca cttccgtcca gagccggatc cggtgagaag
     8161 ttcttcggcg tggtccagaa gttgccatcg acataggcgt acaaattgga gctgaactgg
     8221 ttctggatcg gactcgatgg ctccacatcg accgtgtaaa tcgggaaggt cgtaaactcc
     8281 gcgttcaccg caacaggatt gccggtatcc ggcacaaaga atgtcttcgg gttctccgag
     8341 agtccgcccg gcaaccagaa atacggcgcc tgggcgttga actcatagaa aacccagccg
     8401 ccgaagggca aagccgacgc ggtcagcgtg acctgctcgc gagccacaaa gaaaacatcg
     8461 gacgcgtcgc cggtgtacgt ctgcggctcc ggcgatgcgt ccacaatgcc ggaccccgtg
     8521 gttatcgagg tgtacggtac cagctgaaca aaattcgccg agtacgtcgc gatggccggt
     8581 gatccagtcg ggaaggccgg actcccgttg cccccctgca ccgtgatgct gtgtgtctgc
     8641 tgggtcgagt cgctccaacg tccgtacgtg taatagaacg tcgctgactg cccgctgttc
     8701 tcgatgttcc ccgtcagcgt ctgcaccccg tcggcgacgc tgaggttgtg cgtggaaccc
     8761 actgtccagc tctcggtgca gggcgttgtg cagggaactg aatcgaccag cacctgcagc
     8821 cccaccgggt tcgacgtcac cgtcacctga gtcggcgccg cgccgtaaag ccggcgaatc
     8881 gcttctttgt ctcccgctga ataatcctcg tttccggctc ccggcacacc ttcggttccc
     8941 tgcaggggaa tcccggccgg aatcgtctca atgaccggcc cgccgttccg cgaatccaca
     9001 tacgacggat actgcatcac cgacgcgtag tcgtagggcg agagcagctg ctgattctgc
     9061 gtattgatcg caaaattgcc cgtccagctt cccttgatca cgttgttgta gttcaaggtg
     9121 acatagctgt cgcgatcagt ccgcgtgtgc tcgtgataga ggccgatgat gtgccccatc
     9181 tcgtgcagca gcgtcgccac cgagcagtcg gtgttgcagt tcagattcaa tgctccggca
     9241 aaggccggat aaccaatcgt gtcgacgtcg cctcgtcccc ccgtgccgct cagcgtaatc
     9301 acgacgtacg tggtttggct cgtcctctcc gtccactgca cgacgcctgg aaaatccgca
     9361 ttgaacgtgc tgatggcctg cgccaggttt gggcacgtgt tgcccgcaat gcagccgcca
     9421 tcgtcgtaat agatcacccc actcggccac gtcgatcccg gatcggccac gccaactgac
     9481 tgaggctgag gacgcaggtg cgccagctcg ggtccaggcc gcgactgggc tcgactcgca
     9541 gcggtgaatg ccaacagaag aagcgtcgga acggcaaact gcaccggccg gggaagcgat
     9601 gccttcaggc tgaacatggc ttcaacaccc gcgaaaggag actggagagc agctacggca
     9661 gagtcctatg atcgtgccat gccgcatagg ccctgtcaac gaaagtccaa tcgcgcctga
     9721 accgggctgc ggtaatcgtt tcgattccgt tactgacggg attccctctc gttcgttccg
     9781 cgggttgatg ccgcccacgg ttgcgtttcg gctttgttcg acctttgaaa ccaacttcaa
     9841 ggacaaaacg ctggcacacg gttacctcgg tgcccccgta cgcgccttcc tcctgccggc
     9901 atcaccgaaa acctgccatt cccgctgcta aactccgcag cagaatccgg ccctatgctc
     9961 tttaccgccc gcagcgccgc cgtctacggc atcgacgcca gcatcatcga cgtcgaagtc
    10021 gacttctcga aattccagtc cgagaaggag atcttcaata ccgtcggcct gcccgacgcc
    10081 gccgtccgcg aatcgcgcga ccgcgtgcgc gccgccatcc gcaactccgg cttcgacctc
    10141 ccaccgacac atatcaccat caatctcgcg cccgccgaca tcaagaaaga aggctccggg
    10201 ttcgacctcc ccatggccgt cggcatcctc ggcgcctacg gcgcgctgca ccagcaaaac
    10261 ggcctgcctg acttcctgct catcggtgag ctcggcctcg acggcgccct ccgtcccgtt
    10321 cccggcatgc tgccggttgc cgttgcagcc cgcgagcgcg gcatccgcaa cctggtcatc
    10381 cccaaagcca acgcccgcga agccgctgtc gtcgatggtg tcaacgtcta cccggtcgat
    10441 tccctcctcc aggtacgcga cctcctcaac caggccagca acggcggcat tcaaaccccc
    10501 gccttccatg tggacgcggc tggggtactg ggcgagctcc agcatttctc tctcgatttt
    10561 gcagatgttc gcggacagca ggcggccaag cgcgctctgg aggtcgcagc cgccggcggc
    10621 cacaacatat taatgatagg cccccctggt tcgggcaaaa ccatgctcgc ccgccgtctg
    10681 ccctccatcc tggctccgct caccttcgat gaagcccttc agaccaccaa aatccactcc
    10741 gtagccggtg tgctcgatgc cgaagccgga ctcgtaactc agcggccgtt ccgcgctccg
    10801 caccacacca tctctgatgc tggcctcatc ggcggcggcg tgatcccgcg ccccggcgaa
    10861 gtctccctcg cccacaacgg cgtgcttttc ctggatgaat tgccggagtt cccccgcaac
    10921 gtcctcgaag tgatgcgcca gccactcgaa gatcgccacg tcaccatcgc gcgcgccagc
    10981 atgtctctca ccttcccggc gtcgtttatg cttgcggccg cgatgaaccc ctgcccgtgc
    11041 ggctatttca atgacaagag ccgcgattgc cactgcacgc cgccgatgat ccagcgttac
    11101 gtggccaaaa tctccgggcc tctgatggat cgcatcgaca tccacattga agtgcccgct
    11161 gtacagtaca gggaacttcg tggcgccgcg gctatcgaaa actccgccac cattcgcacc
    11221 cgcgtcctgg gcgcgcgtga gcgccaacgg cagcgttttg ccgcagcaaa agaaaagatc
    11281 ttctccaatg cacaaatggg cacgcgccag attcgcgccc attgcgaact caatcccgag
    11341 gcggaaaaac tcctcgagcg cgccatgctc cagcagggtc tcaccgcgcg cgcccacgac
    11401 cgcatcctta aagttgcccg caccattgcc gatctcgaag gaaccgcctc gctcgcggtg
    11461 tctcacatcg ccgaggcgat tcagtaccgg acgctcgacc gaagttattg ggccggataa
    11521 cccactcttt cagccttccg caacccgccc caaacattca ttcatcctcc ctcccttcct
    11581 gccgatattt ccgtcagcag gacagaatgc accgtgaatt tggctcccat tgtcgccgcc
    11641 ccagccgatg aagaacgtca ggcctccgtt cctctgaata tcgacgccct gcagcccctc
    11701 accgttcagc ccgagcgctg gatgctgggt tccatggtgg gccgagcccc ggtcatgcag
    11761 cacctttttc agcgtatgcg ggccaccgct ccgcattttc gtctggtcaa tgtcgaaggc
    11821 gaacagggaa ccggcaaact ccaggtcgcg caaaccctgc accggattgg cgccgctgcc
    11881 tccggtccct ttgtcccggc gatcgctctc gaattcatcg aggccccgga ctccatttgg
    11941 caggaagccc agggaggtct cctctacctg gcgcgtgtgg acgaattaac caccgaccaa
    12001 cagcggcgcc tccgcgattt tctcgaacgc gcggcgcatg agcgtatgcg cgccgccaaa
    12061 aacctcgctc ctctgcagat cgtcgccgga tcgacccagt cgctccgtcg cctggcaggc
    12121 tccggcgctt tccgctccga cctggccagc catctgaccg ccgtccgctt cggcattccg
    12181 cctctgcgcg atcgcaaaga cgatatcccc ctcctcgccg cactctttct ccgcgactgg
    12241 agccgcgagc acaataaccc tctgcgtggt tttgccgccg gcgtgctgcc ccgcctcact
    12301 tcctatgctt ggcccggcaa cgtccgcgaa ctggaatccg tcgttgccgc cgctgctctc
    12361 gagtgctccg gccaatgggt tcgccccatc gacattcccc ctctgcactc accggctccg
    12421 tcaatgccgg cacccatgcc gctcgatccc ctcgccgacg accctaacct cgaccgcgcg
    12481 attctgcgtc acattacccg catcctcgct cgggcgaacg gcaataaggt ccgcgccgcc
    12541 aggatgctcg gcatcagccg ctccaccctc tatcgcctgc tcgaccccca tcttccggcc
    12601 gactccacta ccgagtaagg cctcaaaatc acgcccgaat tgcacccgat ttctcccctt
    12661 cgttactcct ttcgggttac tgatgcccct ttcagagaca actccgggcc ttcccccaac
    12721 atcgaaattt gagctttaaa cgattcggcg cctcgccgat gccttgtttc aattcggaga
    12781 gcagaaaggt gactcccaaa tccgcaactc ccgggctcag aaggacggca accgtccctg
    12841 actcctctcc gtccagcgac ggccagctca aaccggaccg cctcaaaacc ggaatcgacg
    12901 gcctcgacga tattctgcac ggcggctttc ccgccggtca tctctacctg atcgaaggcg
    12961 atccgggcac cggcaaaacg actctcgctc tccagttcct cctcgaaggc atccggcgcg
    13021 gcgagcgcgt cctctacatc accctgtcgg aatccgctac cgaattgcgg caggtcggtc
    13081 gctctcacgg ctggagtctg gaaaccctcg aaatcgccga gatgattccg cctgacgatg
    13141 atctgcggcc cgaagcccag tacacggtct tccatccctc tgaggtggaa ctggccgaca
    13201 cgatcgcttc cattctgaaa aagatcgacg aaatccagcc tcagcgcctg gtcttcgatt
    13261 cgctggccga attgcgcatg ctcgcccgcg atcctctgaa ataccggagg cagatcctcg
    13321 cgctcaagcg ccatctttca ggcggcaaca tcaccgtcct catgctcgac gaccgcacgt
    13381 ccaactccaa tgacgacctc cagctccaga gcatcgctca cggcgtaatc gtgatgcagg
    13441 gcctcggccg cgactatggt atccgccgcc gccggctcga gattcacaag cttcgtggct
    13501 cggcctttcg cgagggttac cacgactatg tcatccgcac cggcggtctg gacatcttcc
    13561 cccgcctcat cgccgccgag cacaaacctg atttccggcg cagtcctgtg gccagcggat
    13621 tgccgcatct cgacgacttg ctgcgtggcg gcatcgatac cggcaccagc acattgctta
    13681 ccggtccggc aggatgcggc aagtctaccg tcgcgtttca ttacgcctat gcggcggcca
    13741 tgcgcggcga aaaagcaatg atctttacct tcgacgagtc ccgggccacg ctcaccgacc
    13801 gggcccgcgg ccttggcatg gatccggaac ccgttctcca gtctggccgc ctccaggtgc
    13861 agcaggtcga tccggctgaa ctttccccag gcgaattcgt ctctcggatc cgccgcctgg
    13921 tcgacgacgg acacctcagc gtgctcgtca tcgacagcat gaacgggctg ctgaacgcca
    13981 tgccggatga gcagtatctc gcgatgcagt tccacgagct gttctcctat ctcggccagc
    14041 agggcatcgc caccatcatc accctggctc aggccggtgt catcggaact gccatgagcg
    14101 cacctgtgga tgtcagttac ctggccgaca cgatcctgct cttccgctat ttcgagcggg
    14161 ctggagaaat ccgccaggcc atctctgtgg tcaagaagcg cagcggttcg cacgaacgaa
    14221 ccattcgtga gcttttcatg gagccgggcc gggtttcggt gggcaatccc ctgtctgaat
    14281 ttgagggtgt gctcaccggc gttccgcgtc tcatcgccaa acgcgatccc gcgcaggagg
    14341 gcagatctgt ctcctgatat ccaactagaa aagcaggaga accgcaccat catcctcgct
    14401 cctacgggca aggacacctc tctgatcgcg ggactcctcg agcgttgctc catcccctgt
    14461 cttgccgcga aaaccgttga ggaactgtgc gtcgagatcg agcgcggagc aggcgctgcc
    14521 atcattgcag aggaagcgct catgcacggc gccattcata aggtgcgcga agtcatcgag
    14581 caccagccca cctggtcgga tttccccctc atcctgctca ccgtcggcgg gcgagtcacc
    14641 gcggaaagcg agcgccttcg tgagttccgc atgccgctgg gcaatctgtt cctgctcgag
    14701 cgccccattc gccctgagac gctgctcagc acgctcgaaa tagcgctgcg cggccgccag
    14761 cggcagtacc agattcgcga tcagatgcat cagtacgcgc gcgcccagga cgccttgcgc
    14821 cgctctgaga agctcgccgt cactggacgc ctcgccgcca gcatcgctca tgagatcaac
    14881 aatccgctgg aggctgtgac caacctgctg tacctgatgc gcgcctcgga gaaccccgcc
    14941 caggcgcata cctggctggc ccaggccgat caggagctgg cccgcgtcgc cgaaatcacc
    15001 cgccagaccc tgcgcttcta tcgcgagccg agtcatcccg tcgacaccgt ggttgccagt
    15061 gtccttcgct cagtcctcgt tctctacaac tctcgcatcg gtgcggcaaa cgtcgaaatt
    15121 ctgctcgaag tcgaagctgc cgaagctacg gtgctcgcca gccccggcga actgcgtcag
    15181 gtcatcgcca acatcgtcgg caacgcgatt gacgccatgc ggcacggtgg gcgtctgcgc
    15241 ctccggcttt ccgaagaaaa tgacgcatcc gaatcgccac gcgtccgtct taccatcgcc
    15301 gacaccggct ccggcattcc cactgccctg ctgcccacca tcttcgagcc ctttgtcacg
    15361 accaagggag agaccggaac cggcctgggc ctgtgggtga ccggcgaaat catccgccgc
    15421 aatggctggg atatccgcgt gcgcagccgt tgctctccgc atcgctcagg aactgtcttt
    15481 tctatcacta tgccgctccg gtccgcggta gcgcagaccg aatccgccct ggccgtcgcg
    15541 tagcatcctg cgaaaacaac aaattgtttt gcaaaggccc ggcttgggtc gggttgcgcc
    15601 tgctcacacg aacccggatc cagcagctca ggatgtcttc gcacttcaga cgcggcaagg
    15661 cgcctcctat cttgctaatc tgctggcatg aaaaagtact ccttcaccgc cacgatcgag
    15721 gcggcctcgg tgggtggcgg cggcgcttac atcctgttcc cgtacgacgt cgagaaagaa
    15781 tttggcacca ggggccgcgt tcccgtccag gccaccttcg acggcgttcc ctacaccggc
    15841 tcccttgtga agtacggcca gccccagcac atgctcggca tccttaagtc ggtgcgcgac
    15901 cagctcggca agcagcccgg cgataaagtc aaagtcgtac tctggaaaga cgaagcccct
    15961 cgcaccatcg aaacccccgc cgatttcgcc gcgctcctca caaaagaaaa gctcctcgct
    16021 gccttcgaaa agctcagcta cacccaccgc aaggagtacg tccgctggat caccgaggca
    16081 aagaaagaag aaacccgcac cgcccgtctc acaaaatcca tcgccatgct ccgcaaaggc
    16141 gtaaagaccc cggactaacc gtttttcgct ggcagctttt gctaacagca ggctgctaac
    16201 ggctttttgc aaatagcccg ttgccaacag cttttcgctg tccgtctctc gctgccggct
    16261 accggcgctt cttataccct gtacggagtg atgaaactcc gtatcgtcgc ctcccttttg
    16321 ctggcttatt ccgctgcagc cgtcgtcccg caactcccag cacaaaatcc gcccgcagcc
    16381 cagactgccg gcgacgcagc cgcccgcggc aaagccctca acgacctctt cgcccagatc
    16441 tgggaagacc gcctgagcca ctcgcccgag ttcgcctcca ccatcggcga cgaccgcttc
    16501 aacgaccagc tcaccgacta ctccatcgag gcgtataacc agcgtctcgc gcgcggccgc
    16561 gactacatcc tccgcctgag tgcgatcgat accaacggcc ttcctgacca ggaagtcctt
    16621 tcgcgagacc tgatgatccg ggacctcacc gatgatcagg aagagtccgc cttcaagccg
    16681 tgggagatgc ctgtcaatca gttcaacggc ctccatctct ccctggccca gcttgttcct
    16741 cgcctccgtt ttaacagcgc gagagactac gacgactaca tcgcgcgcct cggcaaaatc
    16801 cccgccgcct tccgccagat cagcgacaac atgctcaccg gcgtcgacga tcggcgtgtc
    16861 ccgcccaaat acctcctcga aaaagttctc acccaggtca acgcgctcct cgcgcagaag
    16921 cccgaggaca gccccttcgc tcttcccctg aagaaattcc ccgcctccat ctccgccgcc
    16981 gaccaggccc gctaccgcga agccatcctt accgccattg cgaaacaggt cgaccccgcc
    17041 taccagcgct tcgcgaaatt cctccagggc gtctatatcc ccgcgggtcg ttccgatccc
    17101 ggcgtctggg cccttcccga tggcgacgca tactacgcct tccgcgtgaa gcagagcacc
    17161 accaccgacc tcacccccgc gcaaatccat cagctcggcg tcgatcaggt cgcgcaggac
    17221 gaagccgcca tgctcgtcat cgcgaaaaag ctcggctatc ccaccatcgc cgcgatgcgt
    17281 accgccatcg ccgctaatcc caaactccac cccgccagcc gcgagcagct tctcgacgcg
    17341 taccgcgcag atctcgacaa gatgcgcccc aaactccccg acctcttcgg ccgcctgccc
    17401 aaagctccac tccgggtcga agctgtccct gccttcatgg agaaagacca ggcgcccgct
    17461 tactacgaac agggcacccc tgacggcagc cgccccggca ccgtcttcgt caacacctat
    17521 gactacgagc accactcact cgccaacgtg gaatcggtcg cctaccacga aggcctgcct
    17581 ggccatcacc tgcagatctc catcgcgcag gaactcaccg gccttcccac cttccgcaaa
    17641 tatctgcact acaccgcctt caccgaaggc tggggtctct acgccgagca actcggcaag
    17701 gatgttggct tctatcagga cccctactcg gacttcggcc gcctcgaagg cgacatcttc
    17761 cgcgccatcc gtctcgtcgt cgataccggc gttcattccc aacactggac ccgccagcag
    17821 atggtcgact acttccacgc ccactccggc ctcgacgacg ccaccattaa cgccgagtgc
//
LOCUS       DASZYN010000188         1993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1100091, whole genome shotgun sequence.
ACCESSION   DASZYN010000188 DASZYN010000000
VERSION     DASZYN010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100091"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VHE33_04790"
     CDS             <1..273
                     /locus_tag="VHE33_04790"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HVT96801.1"
                     /translation="CRQNGTGGTDHGHANVMFALGGPVAGGKVYGRWPGMAPEQLYEG
                     RDLAITTDFRQVLAEAADKTLGAQNLEVVFPHSGLQRRNFLNILQV"
     gene            725..>1993
                     /locus_tag="VHE33_04795"
     CDS             725..>1993
                     /locus_tag="VHE33_04795"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HVT96802.1"
                     /translation="MKPAWFAAFAMPLLCTSLLYPQANPANTPATSDSSPDAKAAAAI
                     AAQDSASPAAPSRQDAEIMAKPAGKGEELGEAELKARLTGRPLFLRGLWLGQELHFNM
                     NGDLVGQSPKGSFTLCGVFVDKVRVTKKTVELEGVRFGVHFEGEAPWEQQATSFDRIQ
                     ITPKKKHQTIVIDRQLVVIPKKKKGLFGMGGKEDKAESAKKDGTKTGSKEAAKDDEQE
                     VATVRELPPGATTSPAESAEHLRNALNKIFAPALDGKMIAEMPDYWQYFYQAQDAHKS
                     LVPTDGSLVHPGPGVNGPKILKNVVPVSNDYAQKAEVAGVASYKVILDPNGKILAVAV
                     YRPIGFGLDENAVTAIQKSTFAPAVKDGKAVPSVIDVAVNFRIYSKRTAASTAAEPTG
                     DPNASPVTGKQSMPGLYSQQSAAGSKTQAQS"
BASE COUNT          431 a          505 c          691 g          366 t
ORIGIN      
        1 tgccggcaga atggaacggg cgggaccgat cacggacacg ccaacgtgat gtttgccctg
       61 ggaggacccg ttgcaggtgg gaaggtctac gggcggtggc cggggatggc tccggaacag
      121 ctgtacgagg ggcgcgatct ggcgattacg acagacttcc ggcaggtgtt ggccgaggca
      181 gcggataaaa cgctgggcgc gcagaacctt gaagttgtct tcccacactc cgggttgcag
      241 cggaggaact tcctgaacat tcttcaagta tgagatgaag gactcagcga ggttaatagt
      301 cagctgaaaa acatggctgg gcgcgggccg tgcctttgta ttgcaccaga aacggggcgt
      361 tccaaaacgg tttctgcgac gttacttttg gctggaggtc gatccgaaac gggttgaact
      421 ttcggcggga aaggtgtcgg cttcttgctg tggggtgaac ctggccgtct cccggttttt
      481 ggggcgaggg aattttgggg cgagggacgt tgaggtgcac cgttgccgtc agggtgcatg
      541 gttgagaccg tcgagggctc cgaagcgcag gttcgggcgg gggatattca catctggatt
      601 ccctcccaaa gtgctattgt ggcggcaaat acctgaatga aattctctgg gaagattgcg
      661 gcgcgatctg gcgcggcact cggctgggtt cgggaagcat ctctgtgggg tttgggaacg
      721 tctcatgaag cctgcgtggt tcgccgcttt cgcaatgcct cttctttgta cgtccctcct
      781 ttacccgcaa gccaatcctg ccaacacccc tgcaacgagc gattcctcac ccgatgcgaa
      841 agcggcggct gcgatagccg cccaggacag cgcttcgccc gcggcaccgt cgcggcagga
      901 tgcagagatc atggcgaagc cggcgggcaa aggagaagag ctgggggaag ccgaactgaa
      961 ggccaggctg accgggcgtc cattgttcct gcgcgggctg tggctggggc aggaactgca
     1021 cttcaacatg aacggagacc tggtggggca gtcgccgaag gggtcgttca cgttgtgtgg
     1081 agtgttcgtc gataaagtgc gcgtcaccaa aaaaacggtg gaactggaag gcgttcgctt
     1141 tggagttcat tttgagggag aagcaccctg ggagcagcag gccacgtcgt tcgaccggat
     1201 ccagatcacg ccgaagaaga agcatcagac gatcgtgatc gaccggcagc tggtggtcat
     1261 tccaaagaag aagaagggcc tgttcggaat gggcggcaaa gaggacaagg ctgagtccgc
     1321 gaagaaggat gggacaaaaa ccgggtcgaa agaagccgcg aaagacgacg agcaggaagt
     1381 agccacggtc agggaattgc cacccggagc gaccacctct ccggcggagt cggccgaaca
     1441 tttgcggaat gcgctgaaca agatttttgc gccggctctg gacgggaaga tgatcgcgga
     1501 gatgccggac tactggcagt acttctacca ggcgcaggac gcgcacaaat cgctggtgcc
     1561 gaccgatggc agcctggtgc atccgggacc gggtgtgaac gggccgaaga tattgaagaa
     1621 cgttgtgccg gtatcgaacg actacgcgca aaaggccgaa gtggcgggag tcgcgagcta
     1681 caaagtgatt ctcgatccga atggcaaaat tctggcggtg gcggtgtacc ggccgatcgg
     1741 gttcggcctc gatgagaacg ccgtgacggc aattcagaag tccacgtttg cgccggccgt
     1801 gaaggatgga aaggccgttc cttcggtgat tgacgtggcg gtgaacttca ggatttattc
     1861 gaagcggacc gcggcttcaa ccgcggcgga acccaccggg gatccgaatg catcgccggt
     1921 gacgggcaag cagtcgatgc cggggctgta ctcgcagcag tcggccgcag ggtcgaagac
     1981 ccaggcgcag agc
//
LOCUS       DASZYN010000189         2336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_907038, whole genome shotgun sequence.
ACCESSION   DASZYN010000189 DASZYN010000000
VERSION     DASZYN010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907038"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VHE33_04800"
     CDS             <1..822
                     /locus_tag="VHE33_04800"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVT96803.1"
                     /translation="RSHSFNSVHGRMPSVATGALLANKTMMGIGVSGDGDSASIGIGQ
                     FVHLMRRNLPMIYIIEDNGVYGLTKGQFSATADIGSKLKTGVINDLPAIDTCSLAIQL
                     GATFVGRSFSGDKKQLLTMLKAAIAHKGTVMLDVISPCVTFNDHEGSTRSYKYMQDHE
                     EPVSEVGFVPSFEEIDVEYDPGTTLDVKMHDGSSLRLKKLHEDYNPTDRAIAVKTLME
                     AHDKGEVLTGVFYIDTQKPSFTDLLNLVDEPLATLPQERTRPSRQILDDIMSRLK"
     gene            1038..2003
                     /locus_tag="VHE33_04805"
     CDS             1038..2003
                     /locus_tag="VHE33_04805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HVT96804.1"
                     /translation="MKIILAGGSGQVGTILARHFHQHGHSVVVLSRSVHQSPWRTVHW
                     DGSTLGSWRDELDGSDVLINLAGRSVNCRYTEKNRQEILESRVLPTQALHQAVATLER
                     PPAVWLNASTATIYRHALDRPMDETTGELGGNEPGAPDTWNFSIQVAKAWEMTFFSTP
                     TPGTRKIALRSAMTFSADRGGVFDVLLSLVRHGLGGTNLPGTQFVSWIHEVDFIRAVE
                     FLIDSRELRGALNLASPNPLPNREFLRVLKKAWGARVGLPTPSWMLEIGAFLLRTESE
                     LVLKSRQVVPGRLLEAGFQFRYPTWTQAAHDLVARWRNQETPLQQ"
     gene            complement(2157..>2336)
                     /locus_tag="VHE33_04810"
     CDS             complement(2157..>2336)
                     /locus_tag="VHE33_04810"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HVT96805.1"
                     /translation="KTMTSWPSIRTDLKNAGANVVDQEVATDGQFITSRKPDDIPAFS
                     KAIIGTLSGAKEKAA"
BASE COUNT          488 a          746 c          637 g          465 t
ORIGIN      
        1 cgttcgcaca gcttcaacag cgtccacggc cgcatgccct ccgttgccac gggcgctctg
       61 ctcgcgaaca agaccatgat gggcatcggc gtctcgggtg acggcgactc cgcctcgatc
      121 ggtatcggcc agttcgtcca cctcatgcgc cgcaatctgc ccatgatcta catcatcgaa
      181 gacaacggcg tctacggcct caccaaggga cagttctccg cgaccgccga catcggctcg
      241 aaactcaaga ccggcgtcat caacgacctc cccgcgattg acacctgctc gctggccatt
      301 cagctcggcg ccaccttcgt cggccgctcc ttctccggcg acaaaaagca gctgctgacg
      361 atgctcaaag ctgccatcgc tcacaagggc acggtgatgc tcgacgtgat ctcaccctgc
      421 gtcaccttca atgaccatga aggctcgacc cgcagttaca aatacatgca ggatcacgaa
      481 gagccggtca gtgaagtcgg tttcgtcccc agcttcgagg aaatcgacgt cgaatacgat
      541 cccggcacca cgctcgacgt caaaatgcac gacggctcca gcctccgcct caaaaaactc
      601 cacgaggact acaaccccac cgaccgcgcc atcgccgtca agacactgat ggaagcgcac
      661 gacaagggcg aagtcctcac cggcgtcttc tacatcgaca cgcagaaacc cagcttcacc
      721 gacctgctca acctggtcga cgagccgctg gccaccctgc ctcaggaacg aacgcgtccg
      781 agcaggcaga tcctcgacga catcatgtcc cgcctgaaat agcaacatcg gcagaagcca
      841 cacgaaaaaa gaaggcgcgt ctttggacgc gcctttccgt tgttacgtgc agactcaatg
      901 cgaactcgct gcgttcatcg acaacgaacg gaggggcgaa tcacggcgtt acaatttggc
      961 tcgggatacg atgcagccgg catgcaggtc cattcttacg tgagtgcgtt tcgccatcct
     1021 cgcaggaacg ccgccctgtg aagatcatcc ttgcgggagg gtctggacag gttggaacca
     1081 ttctggcccg gcactttcac cagcatggtc atagcgtggt ggtgctcagc cgtagcgtcc
     1141 accagtctcc ctggcgcaca gtgcattggg atggctccac tttgggttcg tggcgcgacg
     1201 aacttgatgg cagcgacgta ttgatcaacc ttgctggcag aagcgtcaat tgccgctata
     1261 cggaaaagaa tcgccaggag atcctcgaat cgcgcgtgct gccaacacag gccctgcatc
     1321 aggcagtcgc taccctcgaa cgaccgccgg cggtttggct caacgccagc acggcaacga
     1381 tctatcgcca cgcgctggat cggccaatgg acgaaacgac gggagaatta ggcgggaatg
     1441 agccgggtgc ccctgacacg tggaatttct ccattcaggt ggcgaaggcc tgggagatga
     1501 ctttcttttc cacgccgact cctggaacaa ggaagatcgc actgcgcagc gcaatgacct
     1561 tcagtgcgga tcgaggcggc gtcttcgatg tgttgctcag tcttgtccgc cacggcctcg
     1621 gtgggaccaa cttgcccggc acgcaatttg tctcatggat tcatgaagtc gacttcattc
     1681 gtgcggttga gttcctcatc gacagcaggg aactgagggg cgcattgaac ctcgcttccc
     1741 cgaaccctct tccgaaccgg gagttccttc gagtccttaa aaaagcctgg ggtgcgcgtg
     1801 tcggcctgcc gactccctca tggatgctgg aaataggtgc gtttcttttg cggaccgaat
     1861 ccgagttggt gctcaagagc cggcaggttg ttccgggccg gctactcgaa gccggttttc
     1921 agttcaggta tccaacctgg acccaggccg cacacgatct ggtggcccgg tggaggaacc
     1981 aggagacccc acttcagcaa tagctagacg ctgaaaccgg atctgctcaa tcttgttcgc
     2041 cccaggatgt ggaatccggc cttcgcgacg gctgcacggg aaatcgcaaa aacaaagagg
     2101 cgcatccttc ggacgcgcct cttctactgg ctaggctact ggctggtccc ctgcctctac
     2161 gccgccttct ctttcgctcc gctcagggta ccgatgatcg ccttgctgaa tgccggaata
     2221 tcgtccggct tgcggctcgt gatgaactgc ccgtccgttg ccacttcctg gtccaccaca
     2281 ttcgccccgg cgtttttcag atcggttcgt atcgaaggcc atgaagtcat cgtctt
//
LOCUS       DASZYN010000190        13672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_135656, whole genome shotgun sequence.
ACCESSION   DASZYN010000190 DASZYN010000000
VERSION     DASZYN010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 13672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13672
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135656"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VHE33_04815"
     CDS             <1..273
                     /locus_tag="VHE33_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96806.1"
                     /translation="LYILDEPTTGLHPSDVERLLAQLNRLVDSGNTVITVEHDLDIIV
                     AGDWVIDIGPGAGEEGGRIVASGPPQKVADSGTSRTAPYLRALAAA"
     gene            313..588
                     /locus_tag="VHE33_04820"
     CDS             313..588
                     /locus_tag="VHE33_04820"
                     /inference="COORDINATES: protein motif:HMM:NF021452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 domain-containing protein"
                     /protein_id="HVT96807.1"
                     /translation="MITIELAKEKRTEAIASLKKYFEDEIREPLGDLRAGLLLDFFLE
                     EIGPALYNRAIADAQARLAARVADLDGELYADEFQYWVRQAKKKKRT"
     gene            complement(618..2231)
                     /locus_tag="VHE33_04825"
     CDS             complement(618..2231)
                     /locus_tag="VHE33_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96808.1"
                     /translation="MTRALSMLASSLLLLAAPLVAQVHRTDKAFQSDFRTSDRCVACH
                     NGLKTPSGEDVSIGFDWRASVMANASRDPYWQGSVRRESIDHPESTAAIQNECSTCHM
                     PLAHFTAQAEGQQAQVFSHLPLNDVTSKDGPAADGVSCSICHQIEKARLGTPESFNGN
                     VVFASTADAHNRPEYGPFDVDHGHQRVMQSSTNGMVPTLAAHIRDAALCGSCHTLKTQ
                     ALGPGGKEIGSLPEQMPYVEWLHSDYKDVQTCQDCHMPAVREPMPVTAVFGPLREGLH
                     RHVFVGGNFLLEQILHDNHSDLHVEALPQELQAARARTEEFLRTQAAQVTLENEELHD
                     GRLSVAVRVQNLTGHKLPTAYPSRRAWLHVVVTGAGGRKIFESGALHADGSIEGNVND
                     QDPHRYEPHFAQITSPEQVEIFEPILEDSQGQVTTGLLSAVGYLKDNRLLPNGFDKRT
                     AEPDIAVRGKALDDPNFIGGSSMIRYVIDTHGATGPFHVKAELWYQPVGYRWAHNLAP
                     YKASEPQRFVHYYEAQSARSAMVLATAEK"
     gene            complement(2228..3364)
                     /locus_tag="VHE33_04830"
     CDS             complement(2228..3364)
                     /locus_tag="VHE33_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVT96809.1"
                     /translation="MLLLACAGRIATVLVTLTCTAVLTAQTLKHTRPPDAAHGETVYR
                     SGCIACHGDRGSGAPQTSTEFVRPDTFPNFTRCDQTTAEPDITWKAIVVHGGPGRGFS
                     TIMPAFGELLSDSDIDDVIAYLRTMCTNPHWPRGELNLPRALVTEKAYPEDEFVVSTS
                     ANAGGAPGFETHYIHEQRFGVKNQIEIDLPQFDQDQDHKWEHGIGDITFGLKRVLWSS
                     LHTGSIVAVQGSLLPPSGDSKRGFGAGSTTFEPFLSADQLFKTNTWIQFQMGAELPVD
                     PKKSPQTLFYSTAIGQSIAGDHRLGRLWSPMVEFVANRDLVDGAKTDWDVLPEMQVTI
                     SKRQHIRADLGYRAPFTDTADRHGQVSFYILWDWGDGKLWEGWK"
     gene            3674..6169
                     /locus_tag="VHE33_04835"
     CDS             3674..6169
                     /locus_tag="VHE33_04835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT96810.1"
                     /translation="MKNYRLAAVSSWFDSMLVRNLLHEIRYAVRQLLHNLGFTLLAVF
                     TLALAIGASTTIFSWISATLLNPVPGAPDTSRMVTIARGPRNEHPTPPFSYPDYADLR
                     DHTQAFSGLLAYHDDYMAITGVGKPQRIYGALVSANYFDLLGVRPRLGRFLLTEAKAE
                     STGQAEVVLNYDLWRTRFGADPHVIGSSVQINLHPYTIVGVAPKGFHGCKTGLRADVF
                     IPLGRTHQVWGGPETGDRSVPWLNVLGALAPGVDGPQADTALNVAMQHIATEFPDSHR
                     ADNHVYTDPLWRSPFGANVYFAGTLPLLMALAVVLLVLACANVANLLLVRAVGRRREF
                     AIRLSMGCTRMRLVHQLMIETTIVSLAGGGLAVILTLFTARTIPAFLPPTTLPLELDG
                     SVNASVLIVTAIVSLFTSIVAGLIPALRASRLSPMTVLKEEGLSTSAGHRRSHLSAML
                     VVAQIALSTVLLTCAGLFVRSLRIAQVADPGFNPNHVFLATFDLSPFGYSDARSTEFH
                     RQLLAKLRLAPGVESATLADFSPLSFTIHSSDVQPEGYVAQPHESMEIDDGVVGSDYL
                     ATLRTPLLAGRDFNDDDRETTQRVAIVNQAFVDRYWRVSSREALGKQVKFWGSWRTVV
                     GVAANGKYRRLVYDPTPLILVPLWQQPRDQVILHMRVAGDPLRYTTAVQQALADLNPD
                     LPLYNITTLQASMKLGNVFERIVVDFAGSFGILALLLATVGLYGVVAYTTRQRTHEIG
                     IRIALGAEKATIFRQVLYQGLRLTVAGVVVGLGASLFLTRFLRSLLYGVTTNDWPTLV
                     AVVLALASVALVASFLPARRATRVVPVVALHHE"
     gene            6582..10328
                     /locus_tag="VHE33_04840"
     CDS             6582..10328
                     /locus_tag="VHE33_04840"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVT96811.1"
                     /translation="MFKKFALTALTLLLAFFLPHALHAQDNASITGIVTDASGAVIPG
                     VKVTLTNPATGVSYQGITSAEGSYTINEVKPGPGYTATFSHEGFTSVSVTGLYLNVSA
                     VRTQNVSLTVGNVVTTVAVSGSNENVTLDTTDATIGNSFEVQMVNQLPVQIRDTPFAL
                     FTLQPGVAYNPYTGDGSVTGSRADQTHVTLDGLDVDDRGTGQFGNVNGNAPVDSVQEF
                     RGVEAGQTAGYDSAGGGQFQLVTKSGTNQFHGALFEYHRDTNLEANDWFNNNAGVDRA
                     PLIRNQFGGNLGGPILRDKAFFFFEYNGRRDNQGISVEQTVPTASYLAGNISYVNNNA
                     GCSPFSTLQDSPQCISTISASQVATNYDPLGIGQNPALASFIAGRYPAGNDATGGDGI
                     NTTGFRFNAPINRHENDYVARLDYNLTPSQKIFIRGNALSERTADGFNYPTPIQFPGD
                     PVTRSIDDASWAWVVGHNWVIGANKTNQVSYGETRSRLNFPALYNPTGALLWSFGPLT
                     APYLGNYNAQSRVVPVPQLRDDFSWIKGSHTLQFGGTFKYPTPFNNLVLNDFTPTLGL
                     GGSSGLTELDPTLRPADINSSASSFPTNNWDSALVFDLGSFDQIDATYNYNADGSLVA
                     PGTGTDRKYRYYETELYFGDSWKVKPTLTLTYGVRYQLYTVPYETHGIEAIQVDSTTG
                     KPLGFDDYFFKHILPQSAAGVSGPTSVPFLEYVLAGKANHATGYYQPNYKDFAPHVSF
                     AWNPSFDRATVINGSVGLVYDHTIVSAVQNYQDHSSYLFQSGNNIIYGDSSDPEGSLA
                     NDPRFTSINSLAETPAAPAFSSTSIPYVDTTTDPPTPYGLGINTFSTAIDPNLKTPYS
                     IMLNAGVQHSFPGGFLLNVSYAGRLGRRLLAQVDGSQVTDFKDPASGQGYANAFGNIV
                     QELRNGDDPGSIPAEPWFENQVGTGYTQFLASVVSNLFIVGDIADFTQAIAGAGFLNP
                     NVGMASQFSSNDYYTNKGFSTYHGLLTTLHKNAGHGLQFDFNYTWAHSIDNFSLVANG
                     QSNYTGEFICDATKPRECRGNSDFDITQVFNGNFIYDLPFGHGRSFAATTPTWADEII
                     GGWQISGLPTWQTGLPFYANTGAYGASYANLAPAILLSNHTADVASHPHKDPVSGRLL
                     NFKDTAAANAAFVGPIGLTLGQRNELRGPHYTNLDLGVGKTFPLFSENYKLVFRADAF
                     NAFNHPNFDILTSTGANLTSSSFGRLGHMASSPRVLQGALRFEF"
     gene            complement(10383..13043)
                     /locus_tag="VHE33_04845"
     CDS             complement(10383..13043)
                     /locus_tag="VHE33_04845"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HVT96812.1"
                     /translation="MKIPAPRSSSIGFSLALAALLIPAAPTRAADSPDGIPRALAESR
                     AARVSDLHYQLSYILVPHAPTAQATEQIRFNLTNTAEPLLLDFRDGQVSALTLNGAPI
                     PTAVDHGHLVLPAANLHPGPNTVAARFTANIGPAGKAITRFEDKDDGNEYLYTLFVPM
                     DASMAFPCFDQPDLKGRFKLQVQAPKDWTVLSNTASTPQPLGFSGSAATSFEETQPIP
                     TYLFAFAAGPFIDVHPTVGLPHVWVRKSKAPAALAEVPEVQTTAADGIKFLSTYFAQP
                     FPFPKYEMVLIPGFAYGGMEHAGCTFLREESVLFRTAPTDLNRFQRQVLVLHELTHQW
                     FGDFTTMRWFDDLWLKEGFAQYMAYKTLAALHPDLPVWQRFYQSIKPAAYAIDETQGT
                     TPIYQDIPNLNMAKSAYGAIVYSKAPGVLRQLNFVIGDEAFRGGLQAYLAAHNYGNAT
                     WSDLISAFQSATSDSAKSRNLPAWADIWIRHRGMPRVDVAWSCTGPHLAKLTLTQTPV
                     LSGPDSSHDLWPLSTEILLAYPDGTTSTLRADLGDRSVSLDPSKFNPPLSCPAWIFAN
                     NDDHAYGLFPLDQKSREAVMEQLTASAPAMSDVFRRTLLWGSLWDSVRLAELNPADYV
                     HTTLAVLPRERDEALASSLLSRTQTAVHRYVSASAQSELLARGATLAADRMTNDADHD
                     LRIVWFRSLDRFATQPSGATVMKDLLSGKRSIPGVELRQQDRWSLVTTLIAYGDPEAD
                     AYFAAEQKRDPSGDGLKYAWIAQAARPDSATKQKYFDEYLHDSARSEDWIQSSLGAFN
                     YWNQSQLTARYLQPALEALPQVKRERKIFFLVAWLDAFMDGQQSQASLEVVNHYLQTA
                     NPDPDLRLKVLEAMDELQRTVKIRAKYAAP"
     gene            13286..>13672
                     /locus_tag="VHE33_04850"
     CDS             13286..>13672
                     /locus_tag="VHE33_04850"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVT96813.1"
                     /translation="MKTIETVNGSGAGRKLALFVLLALMAWCCTPAVHAQFNASLRGT
                     VADPSGAVIPDATVTLTNADTNASRTAKTDPSGIYTFNQLEPAHYKLTVTHEGFQAKT
                     LEQVQITPEQANTIDVQMQIGAATQTV"
BASE COUNT         2516 a         4307 c         4150 g         2699 t
ORIGIN      
        1 ctgtacatcc tcgacgagcc caccaccggc ctccatcctt ccgacgtgga gcgccttctg
       61 gcccagctta accgcctcgt cgacagcggt aacaccgtga tcaccgtcga gcacgatctc
      121 gacatcatcg tggccggtga ctgggtgatc gacatcggcc cgggggccgg cgaagagggc
      181 ggacgcatcg ttgccagcgg tcccccgcaa aaagttgctg actccggaac cagccgcacc
      241 gcgccgtatc ttcgcgccct tgctgccgcg tgaccttcgc ggccatttaa tctcggtgcg
      301 gaggaattct gcgtgatcac catcgagctt gcaaaggaga agcgcaccga ggccatcgcc
      361 tcgctgaaga aatacttcga ggacgaaatc cgcgagcccc taggcgacct gcgcgccggc
      421 ctcctgctcg atttctttct cgaagaaatc ggtcccgccc tctacaaccg cgccatcgcc
      481 gatgcgcaag cccgcctcgc cgcccgcgtc gccgacctcg acggcgagct ttatgccgac
      541 gagtttcaat actgggtcag gcaagccaaa aagaagaaga ggacctagcc ggcccgatct
      601 cgcttccggt ttcctcctta cttctccgcg gtagccagca ccatcgccga ccgcgccgat
      661 tgcgcttcgt agtagtgaac gaaacgctgc ggctctgatg ccttgtaggg cgcaaggtta
      721 tgcgcccagc ggtagcccac cggctgatac cacagctcgg ccttcacgtg gaacggcccc
      781 gtcgctccgt gggtatcgat cacgtaccgg atcatgctgc tgccgcctat gaagtttgga
      841 tcgtcgagcg ccttccccct gaccgcgatg tccggctcgg ccgtgcgctt gtcgaatccg
      901 ttcgggagca ggcgattgtc cttgaggtat ccgacggctg acagcagccc cgtcgtgacc
      961 tggccctgag agtcctcgag aattggctcg aagatctcca cctgctcggg actcgtgatc
     1021 tgagcgaaat gcggctcgta gcggtgagga tcctgatcgt tgacgttgcc ctcgatggag
     1081 ccgtcggcgt ggagcgcgcc ggattcgaag atcttccgtc cgcccgcgcc ggtcactacc
     1141 acgtgcagcc atgcgcggcg cgatggatac gcggtgggca gcttgtgtcc ggtgaggttt
     1201 tgtacccgca ccgcgaccga caagcggccg tcgtgcagtt cctcgttctc cagcgttacc
     1261 tgggctgcct gggtccggag aaactcctcg gtgcgagctc gagccgcctg aagttcctgg
     1321 ggtagcgcct ccacgtgcag atcgctgtgg ttgtcgtgca gtatttgctc cagcagaaaa
     1381 ttgccgccga cgaagacatg ccggtgcagg ccttcgcgga gcggaccgaa gaccgcagtg
     1441 acgggcatcg gctcgcgcac ggcgggcatg tggcagtcct ggcaggtctg cacgtctttg
     1501 tagtcgctgt ggagccactc cacgtagggc atctgctccg gtagcgagcc gatctctttg
     1561 ccgcccggcc cgagcgcctg cgtcttcagg gtgtggcagc tgccgcacag cgctgcgtcg
     1621 cgaatatgcg ccgccagcgt gggcaccatg ccattggttg acgactgcat gacgcgctga
     1681 tggccgtggt ccacatcgaa cggcccgtac tccggcctgt tgtgcgcgtc ggccgtgctc
     1741 gcaaacacca cgtttccgtt gaatgactca ggcgtcccga gccgcgcctt ctcgatctgg
     1801 tggcagatcg agcaggagac gccatcggcg gcggggccgt ctttactcgt gacgtcgttc
     1861 agtggcagat gcgagaacac ctgtgcctgc tgtccttccg cctgcgcagt gaagtgcgcc
     1921 agcggcatgt gacaggtgga gcactcgttt tggatcgccg cagtggactc gggatggtcg
     1981 atggactcgc gccgcacgct gccctgccag tagggatcgc gcgacgcgtt cgccatgacg
     2041 ctcgcgcgcc agtcgaagcc gatcgataca tcctcgcccg agggcgtctt gaggccgtta
     2101 tggcacgcga cgcaacgatc cgacgtacgg aagtcgctct ggaaggcttt gtcggtgcga
     2161 tggacctgcg ccaccagcgg cgcagccaac agcagcaggg aagaggccaa catcgacagc
     2221 gcccgcgtca tttccaaccc tcccacaact tgccgtcgcc ccagtcccag aggatgtaga
     2281 agctgacctg tccgtgacga tccgccgtat cggtgaatgg cgcgcgatag ccaagatcgg
     2341 cgcggatatg ctgccgcttg ctgatcgtga cctgcatctc gggcagcaca tcccagtcgg
     2401 tcttcgcgcc gtcgacgagg tcgcggttcg cgacgaattc caccatcggg ctccacaggc
     2461 ggcccaggcg gtgatcgccg gcgatgcttt gcccgatggc cgttgaatag aagagcgtct
     2521 gcggcgactt ctttggatcg accggcagtt ctgcgcccat ctggaactga atccaggtat
     2581 ttgttttgaa gagctgatcg gcggagagga acggctcaaa ggtcgtcgag ccggcgccaa
     2641 aaccgcgctt gctgtctccg ctgggcggca gaaggctgcc ctgcacggcc acaatcgaac
     2701 cggtgtggag gctcgaccac agcacgcgct tcaatccgaa cgtgatgtcg ccgatgccgt
     2761 gttcccactt gtgatcctgg tcctggtcga actgcggcag atcgatctcg atttggttct
     2821 tcacgccgaa acgctgttcg tggatgtagt gcgtctcgaa gccgggtgcg ccgccggcat
     2881 ttgccgaggt cgagacaacg aactcatctt ccgggtaggc cttctcggtc acgagagccc
     2941 gcggtaggtt cagctcgcct cgcggccaat gcggattcgt gcacattgtg cgcagatagg
     3001 cgatgacgtc gtcgatgtct gaatcggaga gcaactcgcc aaacgccggc atgatggttg
     3061 aaaacccgcg cccgggtccg ccgtgcacga cgatcgcctt ccacgtgatg tccggttccg
     3121 ccgtggtctg atcgcagcgt gtgaaattgg gaaaggtgtc cggccgcaca aactctgtgc
     3181 tggtctgcgg tgcgccgctg ccccggtcgc cgtgacaggc gatgcatccg gagcggtaga
     3241 cggtctcgcc gtgcgcggca tccggaggcc gggtgtgctt cagcgtctgc gcggtcagga
     3301 ccgccgtgca ggttaacgtg accaggacag tggcgattcg ccctgcacac gccaacagca
     3361 gcatgctctt tggtctcgat ttgcaaaagg gccgcttacc gctcaaggca agaatcgtga
     3421 tcctttcttc atagggtgcg tagatcgtgg tgctccaacg ctatcttagg gcgcgtgcct
     3481 tacaaccatg tcagaaaaga tcagcgatgc cgctcgcatg tgcaactctc cggaaacagc
     3541 ggcttgccca ggaccggcga ccgtgcatgc gatcgggcgg cgggctgcaa gccgcgcccc
     3601 ggcagtggcg atgctcggcg attcggtgtg atacggctca ccgcaccacc ggatggatgt
     3661 tcgtacagtt agattgaaaa attaccgcct tgcggcggtt tcgtcgtggt tcgactccat
     3721 gcttgtgcgc aatctcctgc atgaaatccg gtatgccgtc cgtcagctgc tgcacaacct
     3781 gggctttaca ctccttgctg tctttacgct ggctctcgcc atcggggcca gcacgacgat
     3841 cttcagctgg atcagcgcta ccttgctgaa ccctgttcct ggcgcacccg acaccagccg
     3901 catggtcacg atcgcgcggg gtccgcgcaa cgagcacccc acgccgccgt tctcgtaccc
     3961 cgattacgcc gacctccgcg accacaccca ggcgttttcc ggcctgctcg cgtaccacga
     4021 cgactacatg gcgatcaccg gcgtgggcaa gccgcagcgc atctatggtg cgctggtctc
     4081 cgcgaattac ttcgatcttc tcggcgtccg cccgcgcctc ggccgttttc ttctcaccga
     4141 agccaaagcc gaatcgaccg gccaggccga agtcgttctc aactatgact tgtggcgcac
     4201 tcgttttggc gccgatccgc atgtgatcgg cagcagcgtc cagatcaatc tgcacccgta
     4261 cacgattgtc ggcgtcgcgc ccaaaggctt tcatggatgc aagaccgggc ttcgcgccga
     4321 cgtctttatc ccgctcggca ggacgcatca ggtttggggg gggccggaga ccggcgaccg
     4381 cagcgttccc tggctcaacg tactcggcgc cctcgctcca ggcgtcgatg gccctcaggc
     4441 cgacaccgca ctcaatgttg cgatgcagca tattgcgact gagttccccg attcgcaccg
     4501 cgcggacaat cacgtttaca ccgatccgct gtggcgctcg cccttcggcg ccaacgtgta
     4561 ttttgcggga acgctgcctc tgctgatggc gctggctgtc gttctgctgg tgctggcgtg
     4621 cgcgaatgtt gcaaacctgc tcctcgttcg cgcggtcggg cgcaggcggg agtttgccat
     4681 tcgcctctcg atgggctgca cacgcatgcg cctcgtgcat cagctcatga tcgaaaccac
     4741 catcgtttcg ctcgccggcg ggggcctcgc ggtcatcctg acactcttca ccgcgcgcac
     4801 gatcccggcg tttttgccgc ccacgacgtt gccgctcgag cttgacggga gcgtgaacgc
     4861 gtccgtgctg attgtcaccg ccatcgtttc tctgttcact tccatcgttg cggggctgat
     4921 cccggcgctg cgcgcctccc gcctctctcc catgaccgtg ctcaaggagg aaggcctgtc
     4981 cacgtccgcc ggccatcgcc gctcgcatct ctccgcgatg ctggtcgtcg cgcagatcgc
     5041 tctctctaca gtgctgctca cttgtgccgg actcttcgtc cgcagcctga ggatcgcgca
     5101 ggtcgccgat cccgggttca atcccaacca cgtcttcctt gccaccttcg atttgtcccc
     5161 ctttggctac tcggacgccc gcagcacgga atttcaccgt cagctgctgg ccaaactccg
     5221 tcttgcgccc ggagtcgagt ccgccactct cgcggatttc tcgccgctgt cgttcaccat
     5281 ccactcgtcc gatgtgcagc cggaaggcta tgtggctcag cctcacgaat cgatggagat
     5341 cgatgacggg gtcgttgggt ctgactatct cgccacactc cggaccccgc tcctcgcggg
     5401 ccgcgacttc aacgacgacg atcgcgagac cacgcagcgg gtcgcgatcg tcaatcaggc
     5461 cttcgtcgat cgctattggc gggtctccag cagggaagct ctcggcaagc aggtgaagtt
     5521 ctggggctcg tggcgcaccg tcgtcggcgt tgccgcgaac ggaaagtacc gtcgtctcgt
     5581 ctacgacccc acgcccctca tcctcgttcc gctttggcaa cagccgcgcg atcaggtcat
     5641 ccttcacatg cgggttgccg gcgatccgct gcgctacacc acggcagtgc agcaggctct
     5701 cgccgacctc aatcccgacc tgccgctcta caacatcacc acgctgcagg cgagcatgaa
     5761 actgggcaac gtcttcgagc gaatcgttgt cgacttcgcc ggatcgttcg gcatccttgc
     5821 cctccttctt gcgaccgtcg ggctctatgg tgtggtggcg tacacgactc gtcagcgcac
     5881 acatgagatc gggatccgca ttgccctcgg cgcggaaaaa gccaccatct tccgccaggt
     5941 gctttaccag ggccttcgac tcacggttgc gggcgtcgtg gttgggctcg gcgcgtcgct
     6001 gttcctgact cgattcctgc gcagcctcct gtatggcgtc accacgaacg actggcctac
     6061 gctcgtcgcc gtcgtcctcg cactcgcctc tgtcgcctta gtggcaagct ttcttccggc
     6121 gcgccgtgcc actcgtgttg tgccggtggt cgctcttcat cacgaatagc ccagcggccc
     6181 cggccgcgat gcatgcctct tccccgtttt tcatagacag gcattggctt ttctatggtt
     6241 tgtcgaagtt cctgctgcac tggtcccagt cgctcgtccc cactccgcga tcacgaatca
     6301 gtcatgagtg tcctcttcat cacaaatccc cgacgattcg tcccccctcc ccctccgttg
     6361 gtctcacaaa aaaatatctc gcccgggacg ttgcggaatg ctgtgcgacc gcatttgttg
     6421 cgcactccaa gtggccattg aagtgccttt taaagagcaa agaccagagc gttgtcccct
     6481 ggtgccgatt cgcggcctaa ccctttagtt gcagagtttt ccctcagcat gatcggggat
     6541 tccggcaaat cgcacgacga cgcctgagcg gaggtaaacc cttgttcaaa aaatttgcac
     6601 tcactgcatt gactcttctc ctcgcgttct tccttcccca tgccctgcac gcccaggaca
     6661 acgcttcgat caccggcatc gtgaccgacg cctcaggcgc cgtgattccc ggcgttaagg
     6721 tgaccctgac gaaccccgcc accggcgttt cctatcaggg aatcacgagc gcggaaggct
     6781 catacaccat caatgaggtc aagcccggcc ccggttatac ggccaccttc tcgcacgagg
     6841 gcttcacctc tgtctccgtg acaggcctct atctcaacgt cagcgcggtg cgcacgcaga
     6901 acgtttcgct cacagtcggc aatgtcgtca ccaccgtcgc ggtgtcgggc agcaacgaaa
     6961 acgtcaccct cgataccacc gacgccacca tcggcaacag tttcgaagtc cagatggtga
     7021 accagctgcc ggtccagatt cgcgatacgc cctttgcact gttcaccctg cagcctggcg
     7081 tagcttataa cccttacacg ggagatggct ccgtgaccgg ctcccgtgct gaccagacac
     7141 acgtcacact cgacggcctc gatgtggacg accgcggcac cgggcagttc ggcaatgtca
     7201 acggaaacgc acccgtcgat tcggttcagg aatttcgcgg cgttgaagcg ggccaaaccg
     7261 caggatacga ttcggccggg ggcggccagt tccagctcgt caccaagagc ggcacgaatc
     7321 agtttcacgg cgcgttattc gaataccatc gcgacaccaa ccttgaagcc aacgactggt
     7381 tcaacaacaa cgccggcgtc gatcgcgccc cgctcatccg taaccagttt ggcggcaacc
     7441 tcggcggtcc tatcctccgc gacaaggcct tcttcttctt cgagtacaac ggccgtcgcg
     7501 acaatcaggg aatctcggtt gaacagactg ttcccactgc ctcgtacctg gccggcaaca
     7561 tctcctatgt gaataacaac gcgggctgca gccccttcag cacgctgcag gattcgcccc
     7621 agtgcatcag cacgatcagc gcttcccagg tcgcaaccaa ttacgatcct ttgggaatcg
     7681 gccagaatcc ggcgcttgca tcctttattg ccgggcgtta tcccgccggc aacgacgcga
     7741 caggcggcga cggcatcaac acgaccggct tccgcttcaa tgctcccatc aatcgtcacg
     7801 agaacgacta cgtcgctcgt ctcgactaca acctgacccc gagccagaag atctttattc
     7861 gcggtaacgc actgagcgag cgcaccgcgg acggcttcaa ctatccgacg ccgattcagt
     7921 tccccggcga tcctgttacg cgttccatcg acgacgcaag ctgggcctgg gtggttggtc
     7981 ataactgggt catcggcgcc aacaagacca accaggtgag ttacggcgag acacgctccc
     8041 gcctgaactt tcccgccctc tacaacccga cgggcgctct gctgtggtca ttcgggccgc
     8101 tcaccgctcc ctacctcggc aactacaacg ctcagtcaag agtcgttccg gtgccgcagc
     8161 tgcgagacga tttcagctgg atcaaaggca gccacacctt gcagtttggc ggtaccttta
     8221 aatatccgac gccgttcaat aacctggtcc tgaacgattt cacccccacc ctcggtctgg
     8281 gcggctcgag cggtcttacc gagctcgacc cgaccctgcg ccccgcggac atcaactcaa
     8341 gcgcgagcag cttccccacc aataactggg acagcgccct ggttttcgat cttggcagct
     8401 tcgatcagat cgacgccacc tataactaca acgcggacgg cagccttgtt gctccaggca
     8461 cgggcaccga ccgcaaatac cgctactacg aaaccgagct gtatttcggc gattcgtgga
     8521 aagtgaagcc cacgctcacc ctgacctatg gtgtgcgcta ccagctctac accgttccct
     8581 acgaaacgca tggcatcgaa gccatccagg ttgactccac aaccggcaag cctctcggct
     8641 tcgacgacta cttcttcaag cacatcctcc cgcaaagcgc tgcgggcgtt tccgggccca
     8701 ccagcgttcc attccttgaa tacgtgctgg cgggcaaggc gaatcacgca accggttatt
     8761 accagccgaa ctataaggac tttgcgccac acgtctcctt tgcgtggaac ccgtcgttcg
     8821 atcgcgccac cgtgatcaac ggcagcgtcg gcctcgttta cgaccacacc attgtgagcg
     8881 cggtccagaa ttaccaggac cacagctcct atctgttcca gtccggcaac aacatcatct
     8941 acggtgactc gagcgatccg gaaggttcac tcgccaacga tccccgcttc acctcgatca
     9001 actctcttgc cgagactccg gcggcgcctg ccttctcgtc aacgagcatc ccctacgtcg
     9061 acaccaccac cgatccgccg acgccgtacg gtctggggat caacaccttc agcacggcga
     9121 tcgatcccaa cctgaagacg ccgtactcga tcatgctgaa cgccggcgtg cagcacagct
     9181 tcccgggcgg tttcctgctg aatgtctcct atgcgggacg cctcggccgc agactgctcg
     9241 cccaggtgga cggcagccag gttaccgact tcaaagatcc tgcctcgggc caggggtatg
     9301 cgaacgcctt cggcaacatc gtgcaggagc tgcgcaatgg ggacgatccc ggcagcattc
     9361 cggctgaacc ttggtttgag aatcaggtgg gaacgggcta tacgcagttc ctcgccagcg
     9421 tggtttcgaa tctcttcatc gtcggcgata ttgccgactt cacccaggcg atcgccgggg
     9481 ccggtttcct gaaccccaac gttggcatgg cctcgcagtt ctcctccaac gactactaca
     9541 ccaacaaggg cttctccacc tatcatgggc tgctgaccac cctgcacaaa aatgccggac
     9601 atggtctgca gtttgacttc aactatacct gggcccactc catcgacaac ttctcgctcg
     9661 tcgctaacgg ccaatccaac tacacgggcg agttcatctg cgacgctacg aagccgcgtg
     9721 agtgccgcgg caactccgac ttcgacatca cccaggtgtt caacggcaac ttcatctacg
     9781 atctgccgtt cgggcacggc cgctcctttg cggcgaccac gcccacctgg gctgacgaaa
     9841 tcatcggcgg ctggcagatt agcggcctgc ccacgtggca gaccggcctc cccttctacg
     9901 ccaacaccgg ggcctacggc gccagctacg ccaatctggc tcccgcgatc ctgctcagca
     9961 atcacaccgc cgacgtcgcc agccatccgc acaaggatcc ggtctcagga cgcctgctga
    10021 acttcaaaga caccgcggca gcgaacgccg ccttcgttgg gccgatcgga ctaacgctgg
    10081 ggcagcgcaa cgagctgcgt gggccgcact acacgaacct cgatttggga gtcggcaaga
    10141 ccttcccgct gttcagcgag aactacaagc tggtcttccg cgccgacgcc ttcaacgcct
    10201 tcaaccatcc gaacttcgac atcctgacgt cgacgggggc caacctcacc tcgtcaagct
    10261 tcggccgtct cggacacatg gcctcttccc cgcgcgtgct gcagggagcg ctgcggttcg
    10321 agttctaaag actcacgtga aataccttcg gggctgcctt cgggcggccc ctttttttgc
    10381 ggctacggtg cggcgtactt cgcccggatt ttcaccgtcc gctgcaattc gtccatggct
    10441 tcgagaacct tcaggcggag gtcggggtcg gggttcgcgg tttgcaggta atggttgacg
    10501 acctcgaggg aggcctggga ttgctggccg tccatgaagg catcgagcca ggcgaccagg
    10561 aagaagatct tgcgctcgcg cttgacctgc ggaagagctt cgagagcagg ctgcaggtaa
    10621 cgcgctgtga gttgcgactg gttccagtag ttgaaagcgc cgagggagga ttgaatccag
    10681 tcttctgagc gcgcggagtc gtgcaggtat tcatcaaagt atttctgctt cgtcgcggag
    10741 tcgggacggg cggcctgggc gatccatgcg tatttgaggc catcaccgga gggatcgcgt
    10801 ttttgttcgg cggcgaagta cgcatcggct tcgggatcgc cgtaagcgat cagagtggtg
    10861 acgagggacc agcggtcctg ctggcgaagt tctacgccgg ggatgctgcg cttgccgctg
    10921 agaaggtctt tcatgacagt ggcgccggac ggctgggtcg cgaatctatc gagtgagcgg
    10981 aaccagacaa tgcggaggtc gtgatcggca tcattcgtca tgcgatcagc ggcaagagtg
    11041 gcgccgcggg cgaggagttc agattgcgcc gatgccgaaa cgtaccgatg gaccgcagtt
    11101 tgcgtgcgac tgaggagcga ggaggcgagc gcctcgtcgc gttcgcgggg gaggacggcg
    11161 agcgtggtgt ggacgtaatc ggcgggattg agttccgcaa gacggaccga atcccagagc
    11221 gaaccccaaa ggagcgtgcg gcggaagacg tcggacatcg ccggggcgga ggcggtgagc
    11281 tgttccatca cggcttcgcg ggatttttga tcaagaggga agaggccgta agcgtggtcg
    11341 tcgttgttgg cgaagatcca ggcagggcaa gacagaggcg ggttgaactt gctggggtcg
    11401 agggagacgg agcggtcgcc gagatcggcg cggagagtgc tggtcgtccc atcgggatag
    11461 gcaagcagaa tttcggtgga gagaggccag agatcgtgac tcgaatctgg gccgctgagg
    11521 acgggggtct gcgtgagcgt gagcttcgcg aggtgcgggc ccgtgcagga ccaggcaacg
    11581 tcgacgcgag gcatgccgcg atgacgaatc caaatatcgg cccaggcagg gagattgcgg
    11641 gacttggcgg agtccgaggt tgcggattgg aaggcagaga tcaggtcgct ccacgtcgcg
    11701 ttgccgtaat tgtgcgcggc gagataggct tgcagtccgc cgcgaaacgc ttcatcgccg
    11761 atgacaaagt taagctggcg caggacaccg ggcgctttcg agtagacgat ggcgccgtaa
    11821 gcggacttgg ccatgttgag gttgggaatg tcctgataga tgggcgtggt tccctgagtt
    11881 tcatcgatcg cgtacgccgc gggcttgatg gactgataga agcgctgcca gacgggcagg
    11941 tcgggatgca acgccgcgag cgtcttgtag gccatgtact gcgcgaagcc ttccttgagc
    12001 cacagatcgt cgaaccagcg catggtggtg aagtcgccga accactggtg ggtgagttcg
    12061 tggagaacga gaacctgacg ctgaaaccga ttgaggtcag tgggcgcggt gcggaagagc
    12121 acggattctt cgcggaggaa ggtgcagccg gcgtgttcca tgccaccgta ggcgaagccg
    12181 gggataagga ccatttcgta tttcggaaaa ggaaacggct gtgcgaagta cgtggagagg
    12241 aatttgatac cgtcggcggc ggtcgtctgg acctccggaa cttctgccag cgcggccgga
    12301 gccttggatt tgcggaccca gacgtgggga agaccgacgg tggggtgaac gtcgatgaag
    12361 ggtccggcgg cgaaggcgaa gaggtaggtg gggatgggct gcgtttcttc gaaagaggtt
    12421 gcggcggaac cggagaatcc gagtggctgg ggtgtggacg cagtattgga gaggacggtc
    12481 cagtctttcg gcgcctgcac ctggagtttg aacctgccct tgaggtcggg ctgatcgaaa
    12541 caggggaagg ccatgctcgc gtccatgggg acgaagaggg tgtagaggta ttcgttgccg
    12601 tcgtctttgt cttcaaaacg cgtgatggct ttgccggcag gtcctatgtt ggcggtgaac
    12661 ctggctgcga cggtattggg gccggggtgg aggttcgcag cggggaggac gaggtggccg
    12721 tggtcgaccg cggttgggat gggggcgccg ttaagggtga gggcggacac ctggccgtcg
    12781 cggaagtcga ggaggagcgg ttcagcggtg ttggtcagat tgaagcggat ttgctccgtc
    12841 gcctgagccg taggcgcgtg gggaacaagg atgtagctga gctggtagtg gaggtcagac
    12901 acacgggcgg cacgggattc ggcaagcgcg cgggggattc cgtcgggact gtcggctgcg
    12961 cgtgtggggg cggcagggat gagcagagcg gcgagagcga gcgaaaaccc tatcgatgac
    13021 gagcgggggg caggaatctt caaaggttcg gcctcaaaag cactatagcg ggcggggcaa
    13081 ggtcctgcag aatcatcggc tcgcggcaac gcacgcctgg gcccggcgcg aattggcggg
    13141 atgtggaaaa tgtttgttga ttagttacgg gcatccctga acggtcgcag atacatcccg
    13201 ctaagttgca cttcttttca ggagagccgg tatgcgtgtc atcccccata gcatgcttcg
    13261 cactgcgcgg agtacgaaat catcgttgaa gactattgag acagtgaacg gcagcggcgc
    13321 cggcaggaag ctggcgcttt tcgttcttct cgcactgatg gcatggtgct gcacgccggc
    13381 cgtgcacgcg cagttcaatg catcgctgcg cgggacggtg gccgacccat cgggagcggt
    13441 gatcccggat gcgaccgtga cgctgacgaa tgcggatacc aacgccagcc gcacggcgaa
    13501 gacggatccg agcggaatct acacgttcaa tcaactggag ccggcgcact acaagctgac
    13561 cgtgacccac gagggtttcc aggcgaaaac gctggaacag gtgcagatca cgccggagca
    13621 agcgaacacc attgatgtgc agatgcaaat cggcgctgcg acccagacgg tg
//
LOCUS       DASZYN010000191         2796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_264399, whole genome shotgun sequence.
ACCESSION   DASZYN010000191 DASZYN010000000
VERSION     DASZYN010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264399"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(404..1750)
                     /locus_tag="VHE33_04855"
     CDS             complement(404..1750)
                     /locus_tag="VHE33_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HVT96814.1"
                     /translation="MSVTSLDRTAELKERIQTRQARIGIVGMGYVGLPLALLFSEERF
                     KVTGFDIDARKVTTLNGGGSYIVRIPATEIQLARQEGFSATSDYSQIAQMDIVIICVP
                     TPLNEYHEPDLSYVTGTVKSLAPYVKEGQLIILESTTYPGTTEEVVVPLLEQGNPAGL
                     RVTREASTPGLYVAFSPEREDPGNDTVARRDIPKVIGGCGPAARDLAAAVYGSIFNRT
                     VPVSSPGAAEMTKLLENIYRCVNIALVNELKQLCLRMGIDIFEVIDAAKTKPFGFQAF
                     YPGPGLGGHCIPIDPFYLSWKAKEFDFRTKFIELAGEVNLAMPYFVVESVMEAMSQQG
                     KALRDSRILILGMAYKRDIDDLRESPSLTIIELLQKRGAQVSYNDPFFPTVGHGRKYA
                     LNMTSTPLEKIPDFDCVVIVTDHSQYDYASIVSKAKLVVDTRNATRGIEAKNLVRC"
     gene            complement(1945..>2796)
                     /locus_tag="VHE33_04860"
     CDS             complement(1945..>2796)
                     /locus_tag="VHE33_04860"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HVT96815.1"
                     /translation="VAVTVNQASTTMTLVANGGSANPGSTVTLTATVAPQVGGTPTGT
                     VDFYDGSTLLGSGTLASAAAGAAATFSTTALSSGSHSISATYAGDTNFTGSTANLQGG
                     ITVSPLSLTMTATPGAQSGTAGGTFTYQLAVAPAFAGTPYPAAVTFAAIGGPAGATIS
                     FSPSSLNASDGPKTVTMSVATPVKSAALHPESTGRPLVPVALAFLLLPLAGTKRMRRN
                     GQKLGRFVCLLLLALAGVVATTALSGCGSGSGGATKINNATQYTITVTATSGTVAQSA
                     TVTLTLQ"
BASE COUNT          556 a          851 c          857 g          532 t
ORIGIN      
        1 ggtcaacaca ccgaccagct acaccgacac cacggtgcag agcggagtga cttacaacta
       61 tgaagtgacg agtgtagatg ggtcgggcgt ggaaagcaca ccctcgaacg tctacaccgc
      121 ggtcatccct taggccccca ccactgcaaa acaaaaggcc cgccgaaatt cggcgggcct
      181 ttcttcatgc ttcacgctga gacgagcggt tgaaaacccg gaacagcttc agccctgatg
      241 tggtgtcccg caagttcgtt gaagaactgg ccggcgcttt tgtaacaggg catgacttca
      301 gtcgtgccgc aaacgcgcga gaatcatgtg ggcttcagcc cctgaagcac tcttcgttcc
      361 cctctcatcc tcaacctttt cagagaatgt tcgggacagc aatctagcag cgaaccaggt
      421 tcttcgcttc gatgccgcgg gtggcgttgc gcgtatccac caccagcttt gccttcgaga
      481 cgatgctcgc gtaatcgtac tgcgagtgat ccgtcacgat gaccacgcaa tcgaagtcgg
      541 gaatcttctc cagcggagtg ctggtcatgt tcagcgcgta cttgcgccca tggccaaccg
      601 tggggaagaa aggatcgttg taagagacct gcgcgccgcg cttctgcagc agctcaatga
      661 tcgtgagcga aggcgattcg cgcagatcgt cgatgtcgcg cttgtaggcc atgccgagaa
      721 tcagaatgcg ggagtcccgc agtgcttttc cctgctggct catggcctcc atcacgctct
      781 cgacgacgaa gtaaggcatg gccaggttca cttcgccggc cagctcaatg aacttggtgc
      841 ggaagtcgaa ctccttcgcc ttccaggaaa gatagaacgg gtcgatggga atgcagtgac
      901 cgccaagacc cggaccagga tagaacgcct ggaatccgaa cggcttggtt ttggccgcgt
      961 cgatcacttc gaagatgtcg atgcccatcc gcaggcagag ctgcttcagc tcgttcacca
     1021 gcgcgatgtt cacgcagcgg tagatgttct cgagcagctt ggtcatctcc gccgcgccgg
     1081 gcgaggaaac aggcaccgtg cggttgaaga tggagccata aacggcagca gccaggtcgc
     1141 gagcggccgg tccgcacccg ccgatcacct tcgggatatc gcggcgagcg accgtgtcgt
     1201 tgcctggatc ttcccgctcg ggcgagaagg cgacatacag gccgggcgtg gaagcctcgc
     1261 gcgtaacgcg cagccccgca gggttgccct gctccagcag cggtaccacg acttcttcgg
     1321 tcgtgcccgg atacgtcgtg ctttcaagaa tgatgagctg gccttctttg acgtacggag
     1381 cgaggctctt gacggtgccc gtgacgtagc tcagatccgg ctcgtgatac tcgttcagcg
     1441 gagtcggcac gcagatgatc acgatgtcca tctgcgcgat ctggctgtag tccgatgtcg
     1501 ccgaaaaacc ttcctggcgg gccagctgga tctccgtggc cggaatgcgc acgatgtagg
     1561 acccgccccc gttgagggtg gtcactttgc gcgcgtcaat gtcgaaaccc gtcaccttga
     1621 agcgctcttc gctgaaaagc agggccagcg gcaggccaac atagcccatg ccaacgatgc
     1681 cgatgcgggc ctggcgggtc tgaatacgtt ctttgagttc tgcggttctg tcgagggaag
     1741 tcacggacat gattcacctg aaattcgggc aacatacatt gccgcgaagt ggttagtggg
     1801 gaaaatggga acagcctggc tcttctgtcg tgcccactgc agacctgcac tcagaagagg
     1861 gactgccaac ggcctggaac tgagatcccg aacagacttc ccccgaggat tttgtatcac
     1921 ctcgggggca gccggcaact acaactattg cagcgttagg gtgacggtag cgctttgagc
     1981 aaccgtgccg ctggtcgccg tcaccgtgat ggtgtattgg gtggcattgt tgattttcgt
     2041 ggccccaccc gaaccgctgc cgcaacccga aagcgccgta gtggccacaa ccccggccag
     2101 cgccagcaga agcaggcaaa cgaagcgacc aagcttctgg ccgttccgcc gcatcctctt
     2161 cgtgcccgca aggggcagca gcaggaacgc cagagccacc gggaccagag gccgccccgt
     2221 ggactcagga tgcagcgctg ccgacttgac cggcgtcgcg accgacatcg tgaccgtctt
     2281 cggtccgtca cttgcattca gcgaactggg tgagaaggag attgtggcgc cggccggtcc
     2341 gccgatagca gcgaaggtga ccgcagccgg atacggcgtg ccggcaaagg ccggcgctac
     2401 cgctagctgg tacgtgaagg tgcctcccgc tgtgccggat tgagctccag gggtggcggt
     2461 catggtcaga ctcaaaggcg ataccgtgat cccgccctgc aggttggcag tactgcccgt
     2521 gaaattcgta tcgccggcat acgttgccga gatgctgtgg ctgcccgacg agagcgcggt
     2581 ggtggagaag gttgctgcag cgccagcggc cgcgctcgcc agggttccgc ttccgagcag
     2641 cgtcgatcca tcatagaaat cgaccgtccc cgttggcgtc ccacccacct gcggagcaac
     2701 cgtggccgtc agggtcacgg tgctgccagg attggccgag ccgccgttag ccaccagcgt
     2761 catcgtcgtg ctcgcctggt tgaccgtcac cgcaac
//
LOCUS       DASZYN010000192        12898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_198239, whole genome shotgun sequence.
ACCESSION   DASZYN010000192 DASZYN010000000
VERSION     DASZYN010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 12898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12898
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198239"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2138)
                     /locus_tag="VHE33_04865"
     CDS             complement(<1..2138)
                     /locus_tag="VHE33_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HVT96816.1"
                     /translation="MNFNFGCQNARSSASCPDSPGPKANKSMTCVAGLLALLFGMGVA
                     AQAQTAMYSGSTSAFDTTTSLGPQGIATDASGNLFIADTNTETIYERVRTGPGVYSAP
                     TALPAPTPNYVFLRGLAIDPSGNLWVADYANGSGGQVYEYASGSFASAPIKVGSGWTA
                     PWGIAADASGNVWVADSGANSISEISAGTVTMTIPGSSSGISAPRGIAINSAGNLFVV
                     DGNVGKILMLPSPYTGAATQINTMGFQGAGLIYLDASSNVWLTEYSTNLVHEMMASDS
                     YASILNWGSGLSGPVAVWPSADGTLLVSNFANGNNGGIAQIFTGAVNLGTVAVNSTSA
                     TQTFAFTFTGAGNTTIGSPRVVTKGATGQDFADAGTGTCTTTNGAGNPYVPGSSCTVV
                     VNLKPKAAGPRNGAVQLVNTSGTVLASALVYGNGSGPEVVFPSNTTRLTLGGGFTEPL
                     GLAGDGSGNVYVADRAGNAVKEIPVGCASGGCVVTLGGGFSSPAGVAVDGNGNIYVAD
                     FGNGAVKVMPAGCADSSCVTTLGGGFSHPFGVAVDGSGNVYVADNGMDAVDEMPAGCA
                     SSACVTPLGGGGFGSTTGVAVDGSGNIYVIGSGSVKEMTPGCTSGACVTSLGGGFNTP
                     LGIAVDGIGNVYVGDFGTNEVNEMPAGCSSSSCVTILGSGFNGPAGVMLDNSGNVYVA
                     DKGNTAVKEMTLATPPSLSFAATSIGSQSS"
     gene            complement(2726..3706)
                     /locus_tag="VHE33_04870"
     CDS             complement(2726..3706)
                     /locus_tag="VHE33_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96817.1"
                     /translation="MRRWFCLLVLLSISLIAGSQPRMHDHSAPGTRSVMDAHNCYPYW
                     EWWHDRIDRALSAGTPLAIEQDLYWYTDPKTQQSWSVVAHGAPISGHEPTMEHYFFDR
                     VRPVVERALKEGNHGDWPLITLNLDLKTEEPEHLRAIWALLTKYQDWLTTAAKTNDVA
                     ALQPLTVRPILVLTGESDAQQSVFYDNVPVGARLLVFGAVHTNSKDPMAAPEVLEPEA
                     ASNYRRWWNNPWAVVEQGGQQHAGDWTAADDDRLRALVEHAHAHHLWIRFYTLDGATD
                     KEESCNGWFRSYNFGSLAAAKARWRAAKKAGVDYIASDQYELLGRFLRGK"
     gene            complement(3721..5145)
                     /locus_tag="VHE33_04875"
     CDS             complement(3721..5145)
                     /locus_tag="VHE33_04875"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HVT96818.1"
                     /translation="MPAPNLVLQGEVRPAQNQTYFEVPFTVPAGTHRITVAFHNLGKA
                     EHTVLDLGIADPFRFRGQSGGNKDHFTLSETDATPSYLPGAIPPGRWKLLISVPNIRA
                     GVTSRFRAEVWFNSALDDSSFTEQPLNDKPGWYRGDLHMHDAHSDGSCPSQTGKSVPC
                     PLFLTVEDAVRRGLDFIAITDHNAMSQYDDERELQPYFDKLLMIPGRELTTFHGHANE
                     WGTTRFIDYRVGSKQVPDVNAMFREARSLGAIVSINHPESPSGEICMGCGWTATPAAD
                     MKLVTSIEVVNGGGVPATRFWEEQLRKGYRLTAVGGSDNHRAEWGPEHPASVGYPTTV
                     VYAHDLSVAGILDGIRSGRVFIDLTGSRDRMLEMQAQAGGNAATMGGVLNAPSGQAVK
                     LEVHVAGCSGGSVHFYIDGEDSSALPPQTIAGGDETLHATWQSDGSRHWIRVEAIGAD
                     GHRLLLGNPVYVNWGAAASAANSH"
     gene            complement(5267..8632)
                     /locus_tag="VHE33_04880"
     CDS             complement(5267..8632)
                     /locus_tag="VHE33_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT96819.1"
                     /translation="MRSFILRMMPLLLLAGAICVQAQVNTATLSGLATDPNGAALPHV
                     TVAAKDLDTGYTRTVQTDDAGAYSMQDLPIGQYQITVSDSGFATLNAHLTMTVGQRAR
                     QDFHLQVGSTQQTVNVQSTAPLLSPDDASVGEVIGSVTIEQTPLALRNWDDLLRVVPG
                     VQIARYTQQSGATSAGRVGDFNVNGVHSLQNDFILDGIDNNTFSENVQELSTESAHPS
                     VDTIQEFNTITNPYSAEYGRSPGAVVSVNTKSGSNQFHGLGYEYVRNNYFDANDFFSD
                     RHNLAKPKYNQNQFGGNIGGPVIKDHLFFFFDYEGTRIKQGVLRTNTVPLPNERIGDF
                     SPATAAAVGVKYNTIYDPTTCTPAFSGNNCQPFANNSVPQGSLDSTMQKLMALFPMPN
                     TTTAGSPPDVNNYIRNALLTDFEHNYDARVDWTLSQKDSVFARYNYSNRTRDIPGYLG
                     GLADGTSTSAWGNQILKAHSAVLGWTHIIGQSMVNEFRFGWVRDFSYAQQQPFGLGQF
                     AGDFVPGIPANPALGGGVPLTSFSNFAFEGSPDFLPKRQIPMQYQYNDTLSQVLGRHT
                     LKYGVSLYLPMRNIFQDEPSTRGDLQFTGVFTCQHSAPGKCVSGTGLSYADGLLGYTQ
                     TTALTNVFFVDQRIWMASGFFEDDWKATPKLTLNLGLRYDFATPALEGKDRMADFDPG
                     SGSMVFAKSGSIGDRAIVKPNTKNFGPRVGFAYSPDAKTVVRGGYGIYYTLFERFGSE
                     DEIALNPPFLINKQLVSNTAPVLQPSVGFPSNFLDPTTVDFTNLKAYHIRSVPVNDPE
                     PYVQQWSFGVQRQIGQPWVAEIDYVGTKSTHLDVIRDYNQPLIAGGVSTGVVPYPNFG
                     QIEWTEPIGFGNYNGLQASLTRRMTNGLEVRAAFTHSRSLDNTPQELESNSGDAPDGR
                     SYNTWYGPSDFDIPNRVAFSYDYHLPVGHGHEMLNHGALAWILGNWMTSGLYSYYSGH
                     PFQVNENDGNHNSILDPYGYTTATPNRVGKVRTVGDPDCWFFASKDSACGSKAPSGST
                     DAYTTTAVGAVGNVGRNPLRGPHIGVWDAALLKDFPIRETWNAEARWEVFNVTNTPEF
                     GQPNGNITSGGVGSITSLAGDPRVMQFALRLSF"
     gene            9118..10536
                     /locus_tag="VHE33_04885"
     CDS             9118..10536
                     /locus_tag="VHE33_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96820.1"
                     /translation="MTRASLVERQARDLSPPASGPDPLHPARLEHNPLDSDPRWHLAQ
                     RVVASRHFVRSALLSRFLIYIVGETLCGRAEAITEHAIGVRVFDRPADYRTVEDNIVR
                     NYARQLRRRLADYFAEEGSAEPLHIDIPLGGYVPAFIETAAKKLPAPDVANDRTIPIR
                     VLPEAAVLTAAEPIPTRRFPRGVMFGALFALYTFALVAVVWFAALRLHAPSAAVPHPS
                     VAAEDTSPAAPLWAALFRSDMNTWIVPADAGLNLLEDLSRRPIPLADYISGGAARLAL
                     PAMDAHSADDLRSQRFTSFVDLETITALARLPQFNPERAQLRFPRDLRLDDIRNANAI
                     VLGSVSSNPWAAMAETSANFRIVLGPGMQGARIVNAHPLPGEAETYVSHWNEPDHETF
                     AVISCFPNLSGNGHILVLQGLDVAGTQAAAEALLHPTAIAPILKEATRADGSLRAFEI
                     LLRSTSIQSAAARTSVVGSRVE"
     gene            complement(11450..11629)
                     /locus_tag="VHE33_04890"
     CDS             complement(11450..11629)
                     /locus_tag="VHE33_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96821.1"
                     /translation="MAKIAKTADRKKMMDTTKSTDCPKCSKPTRIVKRVKDRERGVPG
                     GVYISCSACDFYEKL"
     gene            11948..>12898
                     /locus_tag="VHE33_04895"
     CDS             11948..>12898
                     /locus_tag="VHE33_04895"
                     /inference="COORDINATES: protein motif:HMM:NF026048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HVT96822.1"
                     /translation="MVRRAAPDSSQRLRRIVQWTFVALNLWIGAQFLLWVRYFERGGQ
                     GWQVPRPAGVEGWLPIAGLMNLKYFLLTGQAPAMHPAAMFLIVAFLAMSVVLKKSFCG
                     WLCPVGTASETLWKLGRKVFGRNWQMPKGLDYGLRGLKYLLLLFFVSFIAWMPAEALR
                     DFMSGPYGTIADVKMLNFFRDIGWIGAAVIGWLVLSSLFVQNFWCRYLCPYGALMGMV
                     SLLSPVKIRRDAEACIDCGKCSKACPSHLKIDQLVQIRSAECTSCMACVASCPVENAL
                     QLALPPRKADDAVERWRGQAVRPRVAVAILAVIFLGVILFA"
BASE COUNT         2338 a         4253 c         3757 g         2550 t
ORIGIN      
        1 tcgctgctct gcgaaccgat gctcgtggct gcaaagctca ggctgggcgg agtggccagg
       61 gtcatctcct tcaccgccgt attgcctttg tcggcgacat agacgttgcc gctgttgtcc
      121 agcatcacac cggcgggacc gttaaagccg ctgcccaaaa tggtgacgca gctggaggag
      181 ctgcagccgg ccggcatctc attcacttcg ttggtgccga aatcaccgac atacacatta
      241 ccgattccgt ccactgcgat gccgagagga gtgttgaatc caccgcccag cgacgtgaca
      301 caagcgccgg aggtgcaacc gggcgtcatc tccttgactg aaccagagcc gatgacataa
      361 atgttgccgc tcccatctac tgcgacgccc gtggtgctgc cgaaaccacc gccacccagc
      421 ggggtaacgc aagccgagga cgcgcagccg gctggcattt catccaccgc gtccatgccg
      481 ttgtcggcga cgtagacgtt gccgcttccg tcgaccgcta cgccaaaggg atggctgaat
      541 ccgccaccaa gcgttgttac gcagctggag tcggcgcagc cggcaggcat gaccttcacc
      601 gcgccgttgc cgaaatccgc cacgtagata ttgccgtttc catcgaccgc cacgcctgcg
      661 gggctggaga aaccgccacc aagagtcacg acacaaccgc cactcgcgca acccacggga
      721 atttccttca cggcgttgcc cgcccggtca gccacataca cattgccgct tccgtcaccg
      781 gccagtccaa gcggttcggt gaaaccgccg cccagcgtca gacgggtcgt gttgctgggg
      841 aacaccactt ccggccccga gccgttgccg taaaccaggg cgctcgcgag caccgtcccc
      901 gaggtattca ctaactgcac agccccgttc cgcgggccgg ccgctttcgg cttcaaattc
      961 accacaaccg tgcagctgga gccgggcacg tagggattgc cggcaccatt cgtcgtcgtg
     1021 caggttcctg ttcccgcatc ggcgaaatcc tgaccggtcg ctccttttgt gactacccgg
     1081 ggcgatccga tcgtagtgtt gccagcgcca gtgaaggtga acgcaaacgt ctgcgtcgcc
     1141 gaggtggaat taacagcgac ggttcccagg ttcaccgcgc cggtgaagat ctgtgcgatg
     1201 ccaccgttat tgccgttggc aaagttggaa accagcagcg tgccgtcagc cgatggccac
     1261 acggccaccg gaccactcag tccgctgccc cagttcagaa tgcttgcgta gctgtcggag
     1321 gccatcattt cgtgcacgag gttggtggag tattccgtca gccaaacatt gctgcttgca
     1381 tcgagataaa tcaggcctgc accctgaaat cccatggtgt tgatctgagt cgcggcgccg
     1441 gtatagggcg aaggaagcat caaaatcttg cccacgttgc catcgacaac gaagaggttt
     1501 cctgccgagt taattgcaat accgcgcggc gccgagatcc cgctgctgct gccgggaatc
     1561 gtcatcgtaa ccgtgccggc tgaaatttcc gagattgagt tcgcgccgct gtcggccacc
     1621 cacacgtttc ccgacgcatc ggcggcgatg ccccacggag ccgtccagcc gctacccacc
     1681 ttaatcggag cgctggcaaa ggagcccgag gcatactcgt agacctggcc accggaaccg
     1741 ttcgcgtaat cggccaccca caggttgccg ctgggatcga tggcgagccc gcgcaggaag
     1801 acgtagttgg gggttggagc cggaagcgcg gttggcgcac tgtacacgcc ggggccggta
     1861 cgcacgcgtt cataaattgt ttcggtattg gtgtccgcga tgaacaggtt gccgctggca
     1921 tcggtcgcga tgccttgtgg accaagagaa gtcgttgtgt caaacgcgct ggtgctcccg
     1981 ctgtacatag cggtctgcgc ctgtgccgca acgcccatgc cgaacaggag ggccagcagc
     2041 cctgcaacgc acgtcatgga cttgttcgct ttggggcctg gggaatccgg gcagctggcc
     2101 gacgaacggg cgttctggca tccaaaattg aaattcatcg aaacttctcc tgttgaaaca
     2161 aggcagtcat tgcaaaccag ggattggaac ccgctgagtc agggcgaaca agggccattt
     2221 cggggcggcc gagccaaatt tccgatagtt gattcgctgg ttttggggga attttaccgg
     2281 tcctgtttgc gaatgcaata cccttttgga ttggcccgag gggtctttaa atcctctgcg
     2341 gtaaccgaat ttagttacct gtactcgggt acgaatggat attccagcgt tggctgttga
     2401 ttgcaatcga gaaatggaaa ggtccacgta aacagcggac aatggcgcgg tctgcgggct
     2461 tcaggagcgc ttcatcgtcc actcatcgtg aagtcaacgg aggtcgttgt gcctcgattc
     2521 atttggcggc ttgaacaaat acaagaaggg aattaactaa cttacagaaa tggggggcgc
     2581 catcggctac ccggtcccgg ctaacttctt tccgtgccgg acttcgccgg agttagtgtt
     2641 taggtaatgc agaaatggtt acctgcatcc cgccccgcgc ccctgttttc cacagcgtgc
     2701 cacgcccgtc ccgtcctcga gcgctctatt ttccccgcag gaatcgaccc agcagttcgt
     2761 actggtcgct cgcgatgtaa tccacgcccg ccttctttgc cgcccgccag cgcgccttcg
     2821 cagccgccag cgatccgaag ttgtagctcc ggaaccagcc attgcaactc tcttctttgt
     2881 ccgttgcgcc gtccagcgtg tagaagcgga tccacaaatg atgcgcatgc gcgtgctcga
     2941 ccagcgcccg caaccgatcg tcatccgccg cggtccagtc gccggcgtgc tgctggccgc
     3001 cctgttccac cacagcccag ggattgttcc accagcgccg atagttcgac gccgcctctg
     3061 gttcgagtac ctccggtgct gccatcgggt ccttgctgtt cgtgtgcacc gcgccaaaca
     3121 ccagcagtcg tgcgccgacc ggcacattgt cgtagaagac cgattgctgg gcatccgact
     3181 cgcccgtcag gaccaggatc ggacgcaccg tcagcggttg cagcgccgcc acatcattcg
     3241 tcttcgccgc cgtcgtcagc caatcctgat acttcgtcag cagcgcccag attgcgcgca
     3301 gatgctcggg ctcctcggtc ttcagatcca gattcagcgt gatcagcggc cagtcgccgt
     3361 gattgccttc cttcagcgcg cgctccacga ccggccgcac gcggtcgaag aaatagtgct
     3421 ccatcgtcgg ttcgtgaccg ctgatcggcg cgccgtgcgc caccaccgac catgattgtt
     3481 gggtcttcgg gtccgtgtac cagtacagat cctgctcgat cgccaggggc gttcccgccg
     3541 acagcgcgcg gtcgatccgg tcgtgccacc actcccagta gggatagcag ttgtgcgcat
     3601 ccatcacgct gcgagtcccc ggagcggaat gatcgtgcat ccgcggttgc gatccggcga
     3661 tgagtgagat cgacagaaga accaacaggc aaaaccagcg acgcattacg atcctctctc
     3721 tcagtgcgaa tttgcggccg acgccgccgc tccccagttc acgtaaaccg gattccccaa
     3781 cagcagcctg tgtccatccg cgccgatcgc ttccacgcga atccagtggc ggctcccgtc
     3841 cgactgccac gttgcatgca gcgtttcgtc cccgcccgcg atcgtctgcg gaggcagcgc
     3901 gctcgaatcc tcgccatcta tatagaaatg caccgacccg ccgctgcagc ccgccacatg
     3961 aacctcaagc ttgaccgcct ggccgctcgg cgcgttcaga acgccgccca tcgtcgctgc
     4021 atttccgccc gcctgggcct gcatctccag catccgatcg cgcgagccgg tcaggtcgat
     4081 gaacacgcgc cccgagcgaa tcccatccag aatccccgcc accgaaagat cgtgcgcgta
     4141 gaccaccgtc gtcggatacc cgaccgatgc cggatgctcg ggtccccatt ccgcgcgatg
     4201 attgtcactg ccgcccaccg ccgtcagtcg atagcccttg cgcagttgct cctcccagaa
     4261 tcgcgtcgcc ggcaccccgc cgccattcac cacttcgatc gacgtcacca gcttcatatc
     4321 cgccgcgggt gtcgccgtcc acccgcagcc catgcagatt tccccgctcg gcgattccgg
     4381 gtgattgatt gagacgatgg cgcccagaga ccgggcctcg cgaaacatcg cattcacatc
     4441 cggaacctgc ttcgatccca cgcgataatc gatgaaccgc gtcgttcccc actcattcgc
     4501 atggccgtgg aaggtggtca actcgcggcc cggaatcatc agcagcttgt cgaagtaggg
     4561 ctgcagttcg cgctcgtcgt catactgcga catcgcgtta tggtcggtga ttgcgatgaa
     4621 gtccagcccg cgccgcaccg catcctcgac ggtcaagaac agagggcagg gaacggattt
     4681 ccccgtctgg ctggggcaac tgccgtcgct gtgcgcatcg tgcatatgca gatcgccgcg
     4741 ataccagccg ggcttgtcgt tcagcggctg ctcggtgaag gaggaatcgt cgagtgcgga
     4801 attgaaccaa acctcagccc gaaaccgcga cgtcacgccc gcgcgaatat tcggcaccga
     4861 gatcaacagc ttccaccggc ccggcggaat tgctcctggc agatacgacg gcgtcgcatc
     4921 cgtctcgctg agggtgaagt gatccttgtt cccgccgctc tgcccgcgaa accggaacgg
     4981 atcggcgatt cccaggtcga gcacggtgtg ctcagctttg cccaggttgt gaaacgcgac
     5041 cgtaatccga tgcgttcccg ccggcaccgt gaacggcact tcaaaatagg tctggttctg
     5101 cgcggggcgc acctcgccct gcagaaccag attgggagcc ggcatcgttt gcgcggaaac
     5161 cgccgcgccc aacagaccaa cacagagaga gaaccaacgc agtcgcaacg tcatcactag
     5221 aaggctaacc ggagccgggc gtctctgccc ggctccggat cgaaggctag aaagaaagcc
     5281 gcagcgcgaa ctgcatcaca cgcggatcgc cggccagcga agtaatgctg cccacgccgc
     5341 cgctggtgat gttgccattc ggctgcccaa actccggcgt gttcgttacg ttgaagacct
     5401 cccagcgcgc ttcggcattc caggtttcac ggatgggaaa atccttcagc agcgccgcat
     5461 cccacacacc gatatggggc ccgcgcagag gattgcggcc gacattcccc accgcaccca
     5521 ccgcggtcgt cgtgtaggcg tcggtcgagc ccgacggcgc cttgcttcca cacgccgaat
     5581 ccttgctggc gaagaaccag cagtccggat cgccgaccgt ccgcaccttg cccacccggt
     5641 tcggtgtcgc cgtggtgtag ccgtagggat cgaggatcga gttgtggttg ccgtcgttct
     5701 cgttcacctg gaacgggtgc ccactgtagt agctgtagag tcctgaggtc atccagttgc
     5761 ccaggatcca ggccagcgct ccgtgattca gcatctcgtg cccgtgaccg acgggcagat
     5821 gatagtcgta gctgaacgcg acgcggttcg gaatgtcgaa gtcgctcggc ccgtaccagg
     5881 tgttataaga gcggccgtcc ggcgcgtcac cggagttgct ttccagctcc tggggcgtgt
     5941 tgtcgagact gcgcgaatgc gtgaacgccg cccgcacctc gagtccattc gtcatccgcc
     6001 gcgtcaggct cgcctgcagc ccgttgtagt tgccaaatcc gatcggctcg gtccactcga
     6061 tctgtccgaa gttcggatag ggcaccacgc ccgtgctcac tccgcctgcg atcagcggct
     6121 ggttgtagtc gcggatcacg tccagatgcg ttgacttcgt tccgacgtag tcgatttccg
     6181 ccacccacgg ctgcccaatc tgccgctgta cgccaaagct ccactgctgt acgtacggtt
     6241 ccggatcgtt caccggcacc gagcggatgt gatacgcctt caggttcgtg aagtccaccg
     6301 tcgtcggatc gaggaaattc gacgggaacc caaccgacgg ctgcagcacc ggcgccgtat
     6361 tgctcaccag ctgcttgttg atcaggaatg gcgggttcag cgcaatttcg tcttcgctgc
     6421 cgaaccgctc aaacagcgtg tagtagatgc catacccgcc gcgcaccacc gtcttcgcat
     6481 ccggcgaata ggcgaagccg acccgcgggc caaaattctt cgtattcggc ttcacgatgg
     6541 cccggtcgcc gatcgatccg ctcttcgcga ataccatcga ccccgaaccc ggatcgaagt
     6601 ccgccatccg gtctttgcct tccagtgccg gcgtggcgaa gtcgtagcgc agtcccaggt
     6661 tcagcgtcag tttcggcgtg gccttccagt cgtcctcgaa gaatccggac gccatccaga
     6721 tccgctgatc cacgaagaag acattggtca gcgcggtggt ctgcgtgtag cccagcagtc
     6781 cgtcggcata cgacagcccc gtgcccgaca cgcacttgcc cggtgcactg tgctggcagg
     6841 tgaacacgcc cgtgaactgc agatcgccac gcgtgctcgg ctcgtcctgg aagatgttcc
     6901 gcatcggcag atacaaactc acgccgtact tcagcgtgtg ccgccccagc acctgcgaca
     6961 gcgtgtcgtt gtactggtac tgcatcggga tctgccgctt gggcagaaag tccggcgagc
     7021 cctcgaacgc aaagttggaa aacgaagtca gcggaacgcc tccgcccagc gccggattgg
     7081 caggaatccc cggcacaaaa tccccggcaa actggcccag cccgaacggc tgctgttgcg
     7141 cgtaggagaa atctcgaacc cagccaaagc ggaactcgtt caccatgctc tgaccgataa
     7201 tgtgcgtcca tcccagcacc gcactgtgcg ccttcaggat ctgattgccc cacgccgacg
     7261 tcgacgtgcc gtccgccaga cccccgagat atccaggaat atcgcgcgtg cggttcgaat
     7321 agttgtagcg cgcgaacacc gagtccttct gcgaaagggt ccagtccacg cgtgcatcgt
     7381 agttgtgctc aaagtctgtc agcagcgcat tgcggatgta gttgttcacg tcgggagggc
     7441 ttccggcggt cgtggtgttc ggcatgggaa acaatgccat cagcttctgc atcgtggaat
     7501 cgagacttcc ctggggaacg gagttgttgg cgaaaggctg gcagttattt ccggaaaacg
     7561 ccggcgtgca ggtcgtcgga tcgtaaatcg tgttgtactt cacgcccacc gccgccgccg
     7621 ttgccgggct gaagtcccca atgcgctcgt tcggcagcgg caccgtgttc gtccgcagca
     7681 ccccctgctt gatgcgcgtc ccttcgtagt cgaaaaagaa gaacaggtga tccttgatca
     7741 ccggtccgcc gatgtttccg ccgaactggt tctggttgta cttcggcttg gccaggttgt
     7801 gccgatccga gaagaaatcg ttcgcatcga aataattgtt gcggacatat tcgtagccca
     7861 gcccgtggaa ctggttcgat ccgcttttcg tgttcaccga aaccaccgct ccaggactcc
     7921 gtccgtactc cgccgaatag ggattggtga ttgtgttgaa ctcctggatc gtgtccaccg
     7981 acgggtgcgc cgactccgtg ctcagctcct gcacattttc tgaaaatgta ttgttgtcga
     8041 ttccgtccag gatgaagtca ttctgcaggg agtgcacccc gttcacgttg aagtctccca
     8101 cgcgcccggc cgacgtagcc ccgctctgct gcgtatagcg cgcgatctgc acgcccggca
     8161 ccacgcgcag caggtcgtcc cagttgcgca gcgccagcgg cgtctgctcg atggtgaccg
     8221 acccgatcac ctcgcccacc gacgcatcgt cgggcgagag cagcggcgcg gtcgactgca
     8281 cattcaccgt ctgctgcgtc gagcccacct gcaggtggaa atcctggcgc gcccgctgac
     8341 ccacggtcat cgtcagatgc gcattcagcg tcgcaaatcc gctgtcgctc accgtgatct
     8401 gatactgccc gatcggcaaa tcctgcatcg agtacgcgcc ggcatcgtcg gtctgcacgg
     8461 ttcgcgtgta tccggtgtcg agatccttcg ccgccaccgt cacgtgcggc agcgccgccc
     8521 cgttcggatc ggtcgccagt ccggacaacg tcgccgtgtt cacctgggcc tgaacgcaga
     8581 tcgctccggc cagcagcagg agcggcatca tccgcaagat gaaagatcgc atcgggaata
     8641 cctctttctc cccgttcggg gtttctggat gcgcgcaccc gtgcgcccgc accgtttcct
     8701 tcgttgcaat tgagtctggg gaatgaacga cagatgagtt cagagatcga ttgcctgcgc
     8761 cgaaaaggcg cggccatcgc aacgtcacgt tcacgactgc ctccaagcgg gtcgcgggcc
     8821 tggtttctgg agcgccttgc cggccgcgca gaggccgggg gcccgtcagt gccatcctcc
     8881 gccgcctgca ccgctcgcgg aagtgtttac ccttcctggc ccagcgtgca tacacctggg
     8941 tatttccgtt gcaaatgatt cagcaactgt tacttatacg aattgtcaat tctccgtgaa
     9001 tcgcggtttc cctcagcccg ccggtcaccg attcatcgcc ttttcaccgg ggaaccatta
     9061 ccctgatgac ggtctccttc ccagtcggaa cccccacgcc aggagagtga caaagcgatg
     9121 acccgcgcat ccctcgtcga acgccaggcg cgcgaccttt ctcctcccgc ctcggggccg
     9181 gatccgctcc accccgcccg cctcgagcac aaccccctcg acagcgaccc tcgctggcat
     9241 ctcgcccagc gcgtcgtcgc cagccgccac ttcgtccgct ccgccctgct ctcccgcttc
     9301 cttatctata tagttggcga aactctctgc ggccgcgccg aggccatcac cgaacacgct
     9361 atcggcgtcc gcgtcttcga ccggccagcc gattaccgca ccgtcgaaga caacatcgtc
     9421 cgcaactatg cccgccagct tcgccgacgc ctcgccgact acttcgccga agaaggctcc
     9481 gccgagcccc tccacatcga catcccgctc ggcggctatg tccccgcctt catcgagacc
     9541 gcggcaaaaa agctgcccgc tccggacgtc gcaaacgacc gcaccatccc tatccgcgtc
     9601 ctgcccgaag ccgcggttct aaccgccgcc gaaccgatcc ccacccgccg attcccgcgc
     9661 ggcgtgatgt tcggagccct cttcgccctc tacacctttg cgcttgttgc tgtcgtctgg
     9721 tttgcggccc ttcgtctcca cgcgccgagc gccgccgtcc cgcatccttc cgtcgccgct
     9781 gaagacacgt cgcccgcggc gccgctctgg gccgcgctct tccgctccga catgaatacc
     9841 tggatcgttc ccgccgacgc cggcctcaac ctgcttgaag atctctctcg ccgccccatc
     9901 ccgctggccg attacatcag cggcggcgcc gcccgtctcg ctctccccgc catggacgca
     9961 cacagcgcgg acgacctccg cagccagcgc tttaccagct ttgtcgatct cgaaacgatc
    10021 acggcgctcg cccgcctgcc gcagttcaac cccgagcgcg cccagcttcg ttttccccgc
    10081 gacctccgcc tcgacgacat tcgcaacgcc aacgccatcg tgcttggctc ggtcagcagc
    10141 aatccctggg cggcgatggc tgaaaccagc gccaactttc gcatcgtgct cggacccggc
    10201 atgcagggcg ctcgtatcgt caacgctcac ccgctccccg gcgaggccga gacctacgtg
    10261 agccactgga acgagcccga ccacgaaacc ttcgcggtca tctcctgctt cccgaacctg
    10321 tcgggcaacg gacacatcct ggtcctgcag ggacttgacg tggcgggaac gcaggccgcg
    10381 gcggaagccc tgctgcatcc aaccgcgatc gcaccgatcc tgaaagaggc gacccgggcg
    10441 gatgggtcgc tgcgtgcgtt tgagattctg ctaaggtcca ccagcattca gtcggcggcg
    10501 gcaagaacgt cggtggtggg cagccgggtg gagtgagtaa tccgccgaca tcagttgaat
    10561 ggcgacctat gatttgtcat gtcttggctc caaagccacg tttacattgc cacttggctt
    10621 gggctactga cggcagtggt cggatttgcg gtgaggcatg ggccctaaaa ggtttgccga
    10681 cgtgaattgg tcacaggcga ttctgcaatt cagcgcgctt gtggcccttg gcatgtcctt
    10741 cgtgccatgg ttgaacacga ctgataagag cttcgcgcat gccgttctgg gattcctgtt
    10801 gggcacccta atcatcgatt ctggaagaca gcctcgcgca tagccggcac ggagtgccgt
    10861 ccctgctcga tcctgtgcga tgcacccgct ccgagaggcg cactcgcagg atcgaatcgt
    10921 cttttcctgt cccccattgg cgattgctac tccctgacct ttcgtgacct tcgtaccgtc
    10981 cgcccctctg attcttcttg cattgctcct cggcatgagc gaccctaaag acacggggaa
    11041 gtgttcccaa acgaaaggct cgccgcgagg cgagcctttt ttgttgccac cgacaggaga
    11101 aagccttgcg ccagcggcca aacgccattc gcaacgacga cggcgctgcg ggccgggtgc
    11161 gatcatgttg tcaaacttgt aaactgcgct ctctaatttg aaaacaaagt acttacaaga
    11221 ggccctcgaa aaaaaggcac aggtttccgc cccgagggct ggtcaggcaa gcgagcgaaa
    11281 cctaaacgga aagaatcacg gggatggcag cgaaggaggt aatcaatttg tcagtagggg
    11341 tgggggtgac ccctccgcaa gtcgctgatt ctgcgttcag ggaagggggg tcgacccccc
    11401 ttttttccag aatcgcaaag gacgtcgcta tcggctaccg atcacaggct taaagcttct
    11461 cgtagaagtc gcaggcggag caggagatgt agaccccgcc cggcacgccg cgctcgcggt
    11521 ccttcacccg cttcacaatg cgggtcggct tggagcactt ggggcagtcg gtgctcttgg
    11581 tggtgtccat catcttctta cggtcggcgg tcttggcaat cttcgccatg agtggctcct
    11641 gatcctggtt tgggctcctg gtcgctctcg actcagggcg cagcatcctt cgggccttcg
    11701 cggtgattca cctgatgtgc agggagtacg cgggtgggcg gcggaacgcg cttccctgaa
    11761 ttttacacga cccccgacgg agggaaccgg tcctggaggg taaccgggga cgtgatttaa
    11821 gtcactgagg cccgcagccg agcccgctac gttcaaggca gggcagagag tgccggcagg
    11881 agtcgcagag atgaacacgc agacggcagg atcggtcgca gcagggcaga agagacggaa
    11941 gaagacggtg gttcgccgcg ccgcgccgga ctcgtcccag cgcctgcgcc gcattgtgca
    12001 atggaccttc gtcgcgctca acctctggat cggcgcgcaa ttcctgctct gggtgcgcta
    12061 cttcgagcgc ggtggacagg gctggcaggt tccgcgtccg gcgggcgtcg aaggctggct
    12121 gccgattgcc gggctgatga acctgaagta cttcctgctg accggccagg cgcccgcgat
    12181 gcatccggcg gcgatgttcc tgatcgttgc ctttcttgct atgagcgtgg tgctgaagaa
    12241 gtcattctgc ggctggctgt gcccggtggg cacggcctcg gagacgctgt ggaagctggg
    12301 ccgcaaggtg tttggccgca actggcagat gccgaagggt ctggactacg gcctgcgcgg
    12361 cctgaagtat ctgctgctgc tgttcttcgt ctcgttcatc gcgtggatgc cggcggaagc
    12421 gctgcgcgac ttcatgtcgg gaccctatgg aacgatcgcc gacgtcaaga tgctgaactt
    12481 ctttcgcgac atcggttgga tcggcgccgc ggtcatcggc tggctggtgc tgtcgtcgct
    12541 cttcgtccag aacttctggt gccgctacct gtgcccctac ggcgcgctga tgggcatggt
    12601 gtcgctgctg agcccggtga aaatccgccg cgacgccgag gcctgcatcg actgcggcaa
    12661 atgcagcaag gcttgcccgt cgcatctgaa gatcgatcaa ctggtgcaga ttcgctccgc
    12721 cgagtgcacg tcgtgcatgg cgtgcgtcgc gtcctgtccg gtggagaacg cgctgcaact
    12781 ggccctgccg ccgcggaagg ccgatgatgc tgtggagcgc tggcgcgggc aggcggttcg
    12841 tccgcgtgtt gctgtggcga tcctggcggt gattttcctg ggggtgatcc tgttcgcg
//
LOCUS       DASZYN010000193         1776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_714589, whole genome shotgun sequence.
ACCESSION   DASZYN010000193 DASZYN010000000
VERSION     DASZYN010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714589"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..947)
                     /gene="trxB"
                     /locus_tag="VHE33_04900"
     CDS             complement(18..947)
                     /gene="trxB"
                     /locus_tag="VHE33_04900"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HVT96823.1"
                     /translation="MAKIYDVAIIGSGPSGYTSAIYAGRANLSVVMFQGYETGGQLMQ
                     TTDVENYPGFEEGILGPDMMEKFEAQARRFGTEMLTEDVLSVDFSRRPFTLVSDSGEY
                     QARAVIISTGASAQWLGLPSEERLKGRGVSACATCDGFFFKGKAVAVVGGGDTAMEEA
                     NFLTRYASHVTVIHRRNSLRASKIMQDRAMKNPKISFIWESEVAEVLGEEEVKGLRLR
                     NVQTGAEQVLPVQGLFLAIGHKPNTDLFKGIINSDQAGYLVPVEHTMTNIPGVFAAGD
                     VTDHRYRQAVTAAGDGCRAAIDAERWLEAQSAE"
     gene            complement(1133..>1776)
                     /locus_tag="VHE33_04905"
     CDS             complement(1133..>1776)
                     /locus_tag="VHE33_04905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT96824.1"
                     /translation="AVWGVKLPPLEENDLTLLPESSLPDWRLYMAELSTERLLIWRPG
                     LSSSERADLLARLAEVQRSPADIPPPQGISREVALRLDAAPVIDLASARQRACLLGPG
                     DYALLEAFWPGEAAKMLRPEPQPLLGVIVDRRLLTLAHSSRRTVEACELGIDTLPEAR
                     RRGYALAATVAWSASIQAEGLVPIYSAFAANTASLRLAYAAGYRPFARAIMLK"
BASE COUNT          370 a          552 c          510 g          344 t
ORIGIN      
        1 ggtatgcact cttactttta ttcggcgctc tgggcctcca gccagcgttc ggcgtcgatg
       61 gcggcgcgac aaccatcgcc ggcggcggtg acggcctggc gatagcgatg gtcggttacg
      121 tcgccggcgg caaagacgcc ggggatgttg gtcatggtat gctcaaccgg caccagataa
      181 ccggcctggt cgctattgat gatgcctttg aacagatcag tattgggctt gtgaccaatg
      241 gccaggaaca gtccctgcac cggcaagacc tgctccgcgc ccgtctgcac gttgcgcagg
      301 cgcagacctt tgacctcttc ctcgcccagc acttcggcca cttcactctc ccagataaaa
      361 ctgatcttgg gatttttcat ggcgcggtcc tgcatgattt tgctggcgcg cagactgttg
      421 cgccggtgaa tcacggtgac atgagaggca tagcgcgtca ggaagtttgc ctcttccatg
      481 gcggtatcac caccaccaac caccgcgaca gctttgccct tgaagaaaaa gccgtcacag
      541 gtcgcgcagg cgctcactcc acgtcctttc agacgctcct cgctgggcag acccaaccat
      601 tgggcgctgg ctccggtcga gataatcacc gcgcgcgcct ggtactcccc ggaatccgac
      661 accagggtga aaggccgccg gctaaaatcg acagacaaca cgtcttcggt caacatctct
      721 gtaccgaagc gccgggcctg agcctcaaac ttctccatca tatccggccc cagaatgccc
      781 tcctcaaagc cgggatagtt ctcaacatcg gtcgtctgca tcaactgccc gcccgtttca
      841 tacccctgga acatcactac cgacaaattc gcgcgccccg cgtaaatggc cgaggtatac
      901 cccgatggcc ctgaaccaat tatcgcaaca tcatatatct tcgccatagc aattctatcc
      961 tcacccgttc atgtagattg aacgaaactt ctcgtataag ataacacaga aaggccgcgt
     1021 tttatgccag aatgtgtcct tctcacactt atgggtcagc gtggagaaaa gcaggaatag
     1081 accgcgtgta gccgggtacg cccctgcttt tcccatctgc ggaaaccttt ctttatttca
     1141 gcataatggc gcgggcgaac ggacgatagc cagcggcata cgcgagacgc agagaggccg
     1201 tgttggctgc gaaggcactg tagataggca ccagcccttc tgcctgaatg ctcgcgctcc
     1261 aggccacggt ggcggccagg gcatagcccc ggcgccgcgc ttctggcagg gtatcgatgc
     1321 ccagttcgca cgcctcaaca gtgcggcgcg agctgtgggc cagggtgagc aggcgtctat
     1381 caacaatgac gccgagcagc ggctgcggct ccgggcgcag cattttcgcg gcctcgccgg
     1441 gccagaatgc ttcgaggagc gcgtaatcgc caggaccaag cagacaggcg cgttgcctgg
     1501 cggacgcgag atcgataacc ggcgcggcat ccaggcgcag agcgacttcg cggctgatgc
     1561 cctgtggcgg cggaatgtca gcaggcgaac gctggacttc ggccaggcgg gccagcagat
     1621 ccgcacgctc ggaggaagag agaccaggac gccagatcaa gaggcgttcc gtgctcagct
     1681 cggccatgta aagccgccag tctggcagcg aactttctgg caggagcgta agatcgttct
     1741 cttcaagagg cgggagtttg actccccaga cagctt
//
LOCUS       DASZYN010000194        19684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_198387, whole genome shotgun sequence.
ACCESSION   DASZYN010000194 DASZYN010000000
VERSION     DASZYN010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 19684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19684
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198387"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VHE33_04910"
     CDS             complement(<1..839)
                     /locus_tag="VHE33_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HVT96825.1"
                     /translation="MITLLTLLPLIGGLIVLATTKLGDIARVIAMTVATGALAMAGLL
                     WWGLSPANPGMQFEELRPWVPDLGIWYHVGIDGLGALMLVLSALVVLMSLMASWNNEK
                     HGPVYCALVLFLEAGLFGTFSALNFVHWFIFWELSLIPAFFLVRLWGGKGRAKAAAQF
                     FLYTMVGSVALLLAFLAIFLATGTPDQPGHPGHPGVFDFVQLAQMGQTGELTRTLGQN
                     LHWAGSPEHLSMLLFWAVFLGFAVKTAVVPFHTWLPSTYAEASSETTMLLTGAMSKMG
                     VYG"
     gene            complement(836..2932)
                     /gene="nuoL"
                     /locus_tag="VHE33_04915"
     CDS             complement(836..2932)
                     /gene="nuoL"
                     /locus_tag="VHE33_04915"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HVT96826.1"
                     /translation="MTPWIVQNLWLIPVLPMVAAGLCALLKRRERGLSAGLAIGSMTI
                     SLLLSLKAFAEALTHPVVQVKIVDWMQFGDQWIQLGWLLDPLAAVMLVMVTFVGLLIF
                     IYSLGYMAHDDNFGRFFCFLSLFAGAMLGVVIANSLLLLFMCWEVVGLTSYLLIGFWF
                     HRPAAAAAAKKAFITTRVGDLGLLIGMVWLYSQSGTLLFYANGNGCLEQRALTAMAAH
                     GTVVGLAGSTAIGLLIFCGAVGKSGQVPLHVWLPDAMEGPTPVSALIHAATMVAAGVY
                     LMARVYPLLTPGALEVVAWVGAITALFAACIAVAQNDIKRILAYSTVSQLGYMMLGLG
                     VGGVAVGMFHLITHAFFKALLFLGAGSVIHGCTEEQDIRNMGGLRKFMPVTFATYSAG
                     MLALCGFPLFAGFWSKDAILHAAHGWDVSKGPFYLGAIGALLTAFYMTRQMYYVFFGQ
                     KRAPAATVTASAASAHGAGHGVEPAVSPRHGEVCAVEDPHESPKVMTGPLAILAVFAV
                     FLGFVGTPVWPWFQNFLNGRGAAWDGSGFGEPGLISTMLLSSLIVFAGLVLGWWFYGR
                     KPIVNAMDEDALERIVPPVFHALGNRLYVDELYGVTVIALARFFAGFAWWLDRWIFGG
                     LVRLVGWTVTGVSWFDAGVDRFVVNGGFDEGCRELSRGGRLLSRLQNGRVQTYLSVIA
                     GALVVLALWLLWGHRG"
     gene            complement(2929..3240)
                     /gene="nuoK"
                     /locus_tag="VHE33_04920"
     CDS             complement(2929..3240)
                     /gene="nuoK"
                     /locus_tag="VHE33_04920"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HVT96827.1"
                     /translation="MTPLAGCLLVSSLLFSVGLAGALTRRNAILVLAGIELMLNAANL
                     NFIAFWRFGPHPEFLTGLMFVIFSIAVAAAEAAVGLALILAIYRHARTSDVSEITEMK
                     G"
     gene            complement(3240..3785)
                     /locus_tag="VHE33_04925"
     CDS             complement(3240..3785)
                     /locus_tag="VHE33_04925"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HVT96828.1"
                     /translation="MTVSFLILAILTLAGGVAAMTLRRLVHCALALAVSFAGLAGLYL
                     NLGAEFAGLAQLLVYVGAIAILIVFAILLTRSAETELEKVFSPSGIAGVVLALAVFGV
                     LAWAVAGSGVAHAGTGVAAPQASMRDIGFALMQRYVLPLEVIALMLTAAMIGAVILAL
                     RESRTGGPAEPQLPRNPELRA"
     gene            complement(3949..5154)
                     /gene="nuoH"
                     /locus_tag="VHE33_04930"
     CDS             complement(3949..5154)
                     /gene="nuoH"
                     /locus_tag="VHE33_04930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HVT96829.1"
                     /translation="MSATGTADQIFVLAKDWIVAHTPAPWQTLMSIVISVATIIVVFA
                     ALFALTTVFERKGLGRMQNRYGPNRVGPWGVMQPLADALKSLTKEDIVPRRADAVVHY
                     LAPLVLVGVVFLSYAVLPVGRNMVAVDLDSGVLYFFAVGAATELAVFMAGWSSHNKYS
                     LLGAMRAIAQMISYEIPLILSSVAVIMMAGTLSTVKIVEQQARFSGGIAHWNVFTPWG
                     LAGFILFMIAATAETNRSPFDLPEGESEIIAGYFIEYSGFKFALFFLAEYIEMFAVSG
                     LGITLFLGGWSSPVSFLAWIPSWLWFFGKLLAMIFGFIWVRGTLPRLRMDQLMNFAWK
                     FMLPMTLIVIFSAGIWRFLSPGLVGPKPLGGVIRWLVCALVIFVPYLLLARMGRGRRM
                     EKRTYRFAE"
     gene            complement(5151..5819)
                     /locus_tag="VHE33_04935"
     CDS             complement(5151..5819)
                     /locus_tag="VHE33_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HVT96830.1"
                     /translation="MLGEGIIQGLVETARNFFGSYVDKERLTTVQYPEERHTLPEASR
                     NFPFLLYDGADPDAGLRCVACQICEKECPPRCIHIEKSKDKKPDFVGKPQAYPAVFNI
                     DVSVCMSCQICVEVCPFDAIRMDKVYELSTDDRFRGLLYTKQELAKSNEYYQKIHPVE
                     AAETDERRAKEAEEAKAKAAAAEAAAKAKAAAAAAAVPPAGEATGNVAATPAKPAEPK
                     GTPQ"
     gene            complement(5862..7004)
                     /locus_tag="VHE33_04940"
     CDS             complement(5862..7004)
                     /locus_tag="VHE33_04940"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HVT96831.1"
                     /translation="MRDALLDRDIYEPEEGEIMEVSMGPHHPSTHGVFRMDVKLEGER
                     VVRLKPVFGYLHRNHEKIAEHGAYLSAIPYTDRLDYLCSMTNNWGYVLAVEKLAGLQV
                     PERAEYLRVIVAELTRFQNHAGLIGFLLQDMGASGTPLMYAFREREKVLDLFESLSGA
                     RMMCNYMRFGGLRVDATKEWLDQVRALVAQLPGFVDEFEALLRANEILMARAQGTGVL
                     PRELAINASVTGPMLRASGVNHDIRKVDHYGIYDRFSFKVPLGEHGDVYDRYMMRVLE
                     MRETVKILEQALKDIPEGPIMDPKVRLRGLRPKAGEAYGRIESPKGELGFFLISDGSG
                     NPYRYRVRPPSMINLTILEDMCLGHNIADVVIILGSVDIVLGEVDR"
     gene            complement(7001..7636)
                     /locus_tag="VHE33_04945"
     CDS             complement(7001..7636)
                     /locus_tag="VHE33_04945"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HVT96832.1"
                     /translation="MALPSPDEIKAQIEAGVPGASVTIIQNGSPSGQHSLLLDHAHAG
                     GVAKFLRDHAEWRLDYCTLVTGVDWPDTETSEKVKVKKLVKGADGTESEQEVDEVRKT
                     KVPGFLEAVYHLYSMEKKHGPVVLRLRTGNRTDDTRVPSLTPVWRGAEFQEREAFDLY
                     GIVFEGHPDLRRILMWDGFKDYPMRKDYVAPADEIPLVEARTPAPGQETHG"
     gene            complement(7636..8283)
                     /locus_tag="VHE33_04950"
     CDS             complement(7636..8283)
                     /locus_tag="VHE33_04950"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF011394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HVT96833.1"
                     /translation="MADELKELKIELSKNGIFTTTLEELYNWGRKNSVWPLTFGLACC
                     AIEMIATTMARYDLARFGAEVFRPSPRQADLIIVSGTVTKKMAPQVVRLYNQMPEPKY
                     VIAMGACAISGGPFRDGYNVLKGIDRYIPVDVHIPGCPPRPEALIHAFMTLQNKIEEQ
                     ALAGSARPRHLQKDLPSEFPVPEFGEHGLEPPQNDLVFHDAPGYPVSHPDLKVIQ"
     gene            complement(8378..8758)
                     /locus_tag="VHE33_04955"
     CDS             complement(8378..8758)
                     /locus_tag="VHE33_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HVT96834.1"
                     /translation="MMGQAEHPWLTIVVLLVGAVAFGFTPLALAWLWAKFFSPRKPGA
                     EKNATYECGLESKGDSWMGFGASYYIYAIVFLVFDVEAVFLLPFAVAFTGLSLGACLA
                     MLVFLLLLVEGLVWAYGKGVLTWA"
     gene            9184..12816
                     /gene="nifJ"
                     /locus_tag="VHE33_04960"
     CDS             9184..12816
                     /gene="nifJ"
                     /locus_tag="VHE33_04960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HVT96835.1"
                     /translation="MSTPSKTAIFDGNEAAASVAYRFSEVVAIYPITPSSPMAEFADQ
                     WAADGQRNIFGSVPLVAEMQSEGGAAGAMHGALQGGAFATTFTASQGLLLMIPNMYKI
                     AGELTPTVMHVTARSVATHALSIFGDHSDVMGVRQTGYAMLASNSVQEAADLATIAHL
                     ATLEARVPFLHFFDGFRTSHEINKVLPISDDTIRNLLDEKAIRSHRERALTPDRPVLR
                     GSAQNPDVFFQAREACNPYYLACPVIVQDVMDRFARETGRSYRLFDYYGAPDADRVII
                     LMGSASGATEEAIDALNAQGEKLGLLKVRLYRPFAAEAFLTALPETVRKIAVLDRTKE
                     PGASGEPLYQDIVTTISEALSSGRVPLSQFPVIIGGRYGLSSKEFTPAMAKGVFDELA
                     KPHPKNHFTIGIDDDVTHSSLHWDENFTTESAKTVRALFYGLGSDGTVGANKNSIKII
                     GSETPNYAQGYFVYDSKKSGSLTTSHLRFGPEPIRSTYLISRANFIACHGFEFLEKID
                     VLAAAEPNAVFLLNSPYGPSEVWQHLPRTIQKTILEKHIRFYVIDGFTVARENGMGTR
                     INTVMQTCFFAISGVLPRDEAIDQIKKAIKKTYGKRGEAVVQKNFAAVDASLAQLHEV
                     KVPDHVTAKFDILPAIPTQAPAFVRDVLGAMAAGKGDLLPVSALPPGGTFPTGTARWE
                     KRNIAQVIPVWDQDLCIQCGKCVMVCPHAVIRARVYDSSLLPSAPATFKSARPKWRGM
                     DSELYTLQVSPEDCTGCQLCVEVCPAKSKADPAHKALNMQPQQPLRIPERANWEFFES
                     LPLVARDRLSTSQVKDIQLLEPLFEFSGACSGCGETPYIKLLTQLFGDRLMIANATGC
                     SSIYGGNLPTTPYCQNSEGRGPAWSNSLFEDNAEFGLGMRLAVDQQKTYAEGLVAKLA
                     PSLGDDFVHALLEADQTKEQGINAQRERVRELRAKLATIDSEPARSLDAVADTLVRKS
                     VWIIGGDGWAYDIGFGGLDHVLGSGKNVKVLVLDTEVYSNTGGQMSKSTPRGAVAKFA
                     AGGKLTGKKDLAMEMVAYGYVYVARVALGSNDMHTVKAFQEAEAWDGPAIIIAYSHCI
                     AHGYDLTQGLDHQKAAVNSGYWPLMRYNPAVRAVGKNPFFLDSRAPSIPLKDYIYNES
                     RYTMLQRAHPDVAEELLKEATDDVERTWRVYSGRASMPGRESSPNISPRNGHEQPAAP
                     TPQKEE"
     gene            13292..14101
                     /locus_tag="VHE33_04965"
     CDS             13292..14101
                     /locus_tag="VHE33_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT96836.1"
                     /translation="MMNDLSSLPCPPSLSNPVSSPVLSNIQRKGKAHTTTTSNPTSGR
                     QILIVDDDVSLAGFLCSELQGQKYAVDILHDGEAACSLLDEQRHYDLLILDLNLPKLD
                     GIGLIERIRPARPRTPILVLTARSRVEDKVTAFQSGADDCLTKPFSLAELLARVQALM
                     RRNSGLVPNCSQLADLTLLREERRVERNGRRIDLTPREFAILDVMMRNVGRPVSRATL
                     LQEVWNMPGEPSTNIVDVYMKYVRDKVDGPGERRLTHTIRGFGYELRDSDA"
     gene            14190..14816
                     /locus_tag="VHE33_04970"
     CDS             14190..14816
                     /locus_tag="VHE33_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96837.1"
                     /translation="MAQPLTMLRASIEVLAMPLSAGIDRQKYLEVSAGAVERTCHLFA
                     RVQDLVAASMIEAEKAPFDLWSIVANVVEEQRNRLLASGATIAATKTENSMPILGDAA
                     RTEQAIVLVLRLAGELANAGDVIELSGSSSRGFLELCIENSRQRGKPVDSTARLNLAL
                     AEANIASQRGRYEFVEDPFRVSLALPLADRGQPEIETDLNRCAPQSMH"
     gene            14942..16129
                     /locus_tag="VHE33_04975"
     CDS             14942..16129
                     /locus_tag="VHE33_04975"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HVT96838.1"
                     /translation="MATSVLSSSVSDRFEDRLPIDVFVVSSNARLRQDLQTKLTAPRW
                     QVSEAGSGAGALELLRQRNADEAILLLDPVLPDLEPDDFSAIVRDRYPNLQVLMLNSH
                     TGQLLVGSMSPTGVSTMLADEINKGGAAASTAVPVTLPDTSRLTSDHKPRLRSMIGDS
                     DAMLRTFTITRMVAARNTTVLVTGESGTGKDLLAQEVHLISSRRNQPFVVVNCSAIPE
                     PLLEAELFGYTKGSFTGAVQARIGRIHAAHGGTLFLDEIGDMPLSLQSKILRFLEQGE
                     VQRLGGNDNLKVDVRVVAATNADLKGLIAKQQFREDLYYRLSVFPIHLPPLRERMEDL
                     EQLAQAFVAKFAPGTMLSRAALQILEQHRWPGNVRELRNAIERAVILAGMEREIKPEH
                     IVL"
     gene            16222..17430
                     /locus_tag="VHE33_04980"
     CDS             16222..17430
                     /locus_tag="VHE33_04980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HVT96839.1"
                     /translation="MNEPNVAVLHSPQLLADAFSEFISASATLEASYRDLQSEVAHLS
                     IELAERNAELTRSLEDNNRMRATLQQMIDSMPCGVLVLDAAESIVTMNPEGHRLLGRG
                     SARARTLHDLSEISRIDFEALITETEGQRDNEVCLTAGSKKRWLAIGRRELDGAPAAR
                     FQSIWILRDITADKLAEQERESARRATTLAEISTILAHEIRNPLASLELFAGLIEEDR
                     GGTEEWVANLRAGIRTLSGTVNNVLSMNGDGHKQLAPVNLSACAASGVEFVLPIARQA
                     GVKLQFSPAQTSITVRGNEDALRQILLNLIANAIRHTPVGGLVEVAAKAETRHGAIRG
                     IVTVRDNGCGIPQDQLAHIFEAGFSGTGESPGLGLAVCKRLMAQHHGEIRVTSRVDQG
                     TEFELEFSAI"
     gene            17427..18824
                     /locus_tag="VHE33_04985"
     CDS             17427..18824
                     /locus_tag="VHE33_04985"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HVT96840.1"
                     /translation="MTGETVSSVMVVDDEPGIRTALRANFLRHGWRVETASGVREAIR
                     NLETREYDLVVTDIRMPDGSGMEVMRAARKTSSSTAVILLTAYGSVPDAVTAMRDGAL
                     DYLTKPIPFDQLQATAAQVMQRAQQMNRNEGVVPTVEPETGNGQIVGGSSLLMRALQR
                     ARAAANTGADVLIEAESGTGKELLARFIHDASDRSQKPFVAVNCAAVPETLLESELFG
                     HGRGAFTGAVASRPGKFESANGGTILLDEIGEMPLNLQPKLLRALQEREFERLGEGRT
                     VKVDIRVIATTNVSLAEMVERGQFRADLYYRLNVIPLSLPPLRERRDDIPMLARHFAE
                     QFAGTTGALRPRLHPDFIERLQSHSWPGNIRELANFMRRVITLSGTLEIDARCFEMEF
                     QGISAPQRTVPAKTASIPAPAGTPIRELERMHLENTLALTDGNRTHAAAMLGISLRTL
                     RNKIREYGLPPRRYA"
     gene            18825..19169
                     /locus_tag="VHE33_04990"
     CDS             18825..19169
                     /locus_tag="VHE33_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HVT96841.1"
                     /translation="MADVSMLEMLSGYLKITTDRQQVIASNMANVDTPGYHTRDVNFR
                     QAMQEVMNPGGEPGMETTSLEIQGLPERADGNNVNIDRESMLLSETQLQYQMGVQLIK
                     ERFHTLLTAIKD"
     gene            19181..19630
                     /gene="flgC"
                     /locus_tag="VHE33_04995"
     CDS             19181..19630
                     /gene="flgC"
                     /locus_tag="VHE33_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HVT96842.1"
                     /translation="MGFWDMMSISASALAAERQRAEVTSANLANAQTTHTEGGSGPYV
                     RKEVVFSATNASPFQTIFNRAGHFGAPAGSVELSGVVEDSAPPVMRYEPGNPDANKEG
                     YVAYPAINPITEMVDLMDATRAYQLNASAVTSAKQMVQESIDILKTT"
BASE COUNT         4117 a         6663 c         5364 g         3540 t
ORIGIN      
        1 aatccataga cgcccatctt cgacatcgcg ccggtgagca gcatcgtcgt ctcgctggaa
       61 gcctcggcat aggtcgaggg cagccaggtg tggaagggca ccaccgccgt cttcaccgcg
      121 aatcccagga aaaccgccca gaacagcagc atcgaaaggt gttccgggct cccggcccag
      181 tgcagattct gtcccagcgt ccgcgtcaac tcgcccgtct gtcccatctg ggccaactgc
      241 acaaagtcga agacgcctgg gtgtccagga tgtcccggtt gatccggcgt gccggtcgcc
      301 aggaaaatcg ccagaaacgc cagcagcaac gccacgctcc ccaccatcgt gtagaggaag
      361 aattgcgcgg cagcctttgc ccgtcctttg ccgccccaga ggcgcaccag gaaaaatgcc
      421 gggatcaggc tcagctccca gaagatgaac cagtgcacaa aattcagcgc gctgaatgtg
      481 ccaaacaatc ccgcttcgag aaatagcacc agagcgcagt acaccggccc atgcttctcg
      541 ttattccacg aagccatcag cgacatcagc acaaccagcg cgctcagcac gagcatcagc
      601 gcgcccagcc catcgatgcc cacgtgatac cagatcccca gatccggcac ccacgggcgc
      661 agctcctcga actgcatgcc aggattcgcc ggcgacaatc cccaccacag caaccccgcc
      721 atcgccagcg cacccgtcgc gaccgtcatc gcgatcaccc gggcgatatc gcccagtttg
      781 gtcgttgcca gaacgatcag acctccgatg agcggcagca gtgtgagcag cgtaatcatc
      841 cgcgatgccc ccacaacaac cacagcgcca gcaccaccag tgctccagcg atcacgctga
      901 gataggtctg cacccgcccg ttttgcagtc gcgacagcag ccgtccgccg cgcgacaact
      961 cccggcatcc ctcgtcaaaa ccgccgttga cgacgaaccg atcgacaccc gcatcgaacc
     1021 agctcacacc cgtgaccgtc cagccgacca ggcgtaccag gccgccgaag atccagcggt
     1081 caagccacca tgcgaacccc gcaaagaagc gcgccagggc gatcaccgtc acgccgtaga
     1141 gttcatccac atagagtcga ttgcccagcg catgaaacac cggcggaacg attctctcca
     1201 gcgcatcctc gtccatcgcg ttcacaatcg gcttccgccc gtagaaccac caacccagca
     1261 cgaggccggc aaaaacaatc agcgacgaga gcagcattgt cgatatcaga ccgggctcgc
     1321 cgaatcccga tccgtcccac gcagcgccgc gcccattcag gaaattctgg aaccaaggcc
     1381 acacgggcgt ccccacaaat cccaggaaca cagcaaacac ggccagaatc gccagcggtc
     1441 ccgtcatcac cttcgggctc tcgtgcggat cctcaaccgc gcacacctcg ccatgccgtg
     1501 gcgatacggc cggttccact ccatgccccg ccccatgcgc ggatgcagcc gaagccgtca
     1561 ccgtcgccgc gggcgcgcgt ttctgcccga agaagacgta gtacatctgc cgcgtcatat
     1621 agaacgcggt cagcaacgcg ccgatcgcgc ccagatagaa cggccccttc gacacatccc
     1681 atccgtgagc cgcgtgcaga atcgcgtctt tgctccaaaa cccggcaaac agtgggaacc
     1741 cgcacagcgc cagcatgccg gcggaatacg tcgcaaaagt caccggcatg aatttccgca
     1801 gcccgcccat attgcggatg tcctgctctt cagtgcaccc atgaatcacc gaacccgcac
     1861 ccaggaagag cagcgccttg aagaacgcat gcgtaatcag gtggaacatg cccaccgcaa
     1921 cgccacccac gcccagtccc agcatcatgt aacccagttg cgacaccgtc gaataggcca
     1981 ggattctctt gatatcgttt tgcgccacgg caatgcaggc cgcgaacagc gccgtgattg
     2041 cccccaccca tgccaccact tccagcgccc ccggggtcag cagcggatag acgcgcgcca
     2101 tcagatacac gcccgcagct accatcgtcg ccgcatggat cagcgcgctc accggcgtcg
     2161 gaccttccat cgcatcgggc agccatacat gcagtggcac ctggcccgac ttgcccaccg
     2221 ccccacagaa gatcagcagg ccaattgcgg tggaacccgc caggccgacg accgtcccgt
     2281 gcgccgccat cgccgttaga gcgcgctgct cgaggcagcc atttccgttg gcgtagaaca
     2341 gcagcgtgcc actctgcgaa tagagccaca ccattccgat cagcagcccc agatcgccca
     2401 cgcgcgtcgt gatgaacgcc ttcttcgccg ccgcagccgc cgccggccga tggaaccaga
     2461 aaccgatcag caggtaggac gtgagcccaa cgacttccca gcacatgaac agcagcagca
     2521 ggctgttcgc gatcaccaca cccagcatcg cgcccgcaaa cagcgacaga aaacagaaga
     2581 accggccgaa attgtcatcg tgcgccatgt agccgaggct gtagatgaaa atcagcagtc
     2641 ccacaaacgt gaccatcacc agcatcaccg cggccagcgg atcgagcagc catcccagct
     2701 ggatccactg atcgccaaac tgcatccagt caacgatctt cacctgcact accggatgcg
     2761 tcagcgcctc ggcgaacgcc ttgagggaca gcagcaggga aatcgtcatc gacccaattg
     2821 ccagccccgc ggaaagcccg cgctcccgac gcttcaataa ggcacacaaa cccgccgcca
     2881 ccatcggcag cacgggaatc agccacaggt tctgcacgat ccagggcgtc atcccttcat
     2941 ctccgtaatc tcgctcacat cgctggtacg cgcgtgacga tagatcgcca ggatcagcgc
     3001 cagccccacg gctgcttcag cggcggcgac ggcgatcgag aagatgacga acattagccc
     3061 tgtcaggaat tcaggatgcg gtccaaaccg ccagaacgcg atgaagttca ggttcgctgc
     3121 attcagcatc agctcaatgc cggccagaac cagaatcgcg ttccggcgtg tcaatgcgcc
     3181 ggccaggccg accgagaaga gcagagagga aaccagcaaa caaccagcga gcggagtcat
     3241 caggcacgca gctccggatt ccgcgggagc tgcggctcgg ccggcccgcc ggtgcgcgat
     3301 tctcgaagcg ccagaatcac cgccccaatc atcgcggcgg tcagcatgag tgcaatcacc
     3361 tcgagcggca acacgtaacg ctgcatcagg gcgaagccga tgtcccgcat cgaggcctgc
     3421 ggtgcggcga cccccgtgcc ggcgtgcgcc acgccgctgc cggccaccgc ccacgccagc
     3481 acgccgaaca ccgccagcgc cagcacgact ccggcgatcc cggaggggga aaagactttc
     3541 tccagttcgg tctcagcgct gcgggtcagc aaaatggcaa agacgatcag aatggcaatc
     3601 gcacccacgt agaccagcaa ctgcgccagt cccgcgaatt ctgcgcccag attcagatac
     3661 aaacccgcca gtccggcgaa ggaaaccgcg agcgccaggg cgcagtgcac cagacgccgc
     3721 aacgtcatgg cggccacacc tccggccagc gtcagaatcg cgagaatcag gaaagaaacc
     3781 gtcattccta tagatacttg gttcggtaca aagtccctgc accggacttg cgactggttc
     3841 ccgggtcgcg acgacatccg tcgcgccgag cccctcctac aatttcacgg cagcgcccgt
     3901 gcgactgtga ctcatatcac cagccggctc gaccgctccg cacccgtgtt actccgcgaa
     3961 gcgataggtt cttttctcca tcctgcggcc gcgtcccatg cgcgccaaca ataaataggg
     4021 aacaaaaatg accagtgcac agaccagcca gcgtatcaca ccacccagcg gcttcggtcc
     4081 cacgaggccg gggctaagaa accgccagat gcccgccgaa aaaatgacaa tcagcgtcat
     4141 cggcagcatg aatttccagg cgaaattcat cagctgatcc atgcgcaggc ggggcaaagt
     4201 gccgcgcacc cagatgaacc cgaagatcat tgccagcagc ttgccgaaga accacagcca
     4261 ggagggaatc cacgccagga aactcaccgg cgacgaccag ccgcccagga acagggtgat
     4321 gcccagcccg ctcaccgcga acatctcgat gtattcggcg aggaagaaca gcgcgaactt
     4381 gaagcccgaa tactcaatga aatatccggc aatgatctcc gactcgcctt ccggcagatc
     4441 gaaggggctg cggttcgtct ccgccgtcgc cgcaatcatg aagaggatga atccggccag
     4501 tccccacggt gtaaagacat tccagtgcgc gatgcccccc gagaaccgcg cctgctgttc
     4561 cacaatcttc accgttgaca gtgtccccgc catcatgatc accgccaccg acgacaggat
     4621 cagcggaatc tcgtagctga tcatttgcgc gatggcgcgc atcgccccca gcagagagta
     4681 tttgttgtgg ctcgaccagc ccgccatgaa tacggcaagc tcagtcgcgg cgcccactgc
     4741 gaagaagtac agcacaccgc tgtcgaggtc gacggccacc atgttgcggc cgaccggcag
     4801 caccgcgtaa ctcagaaaca ccacgcccac cagaaccaga ggcgccagat agtgcaccac
     4861 agcgtcggca cgtctcggaa cgatatcttc cttggtgagc gacttcagcg catcggccag
     4921 cggctgcatc acgccccacg gtcccacacg gttcggtccg tagcgattct gcattcgccc
     4981 cagccccttg cgttcgaaca ccgtggtgag cgcaaacaac gcggcaaaga cgacgatgat
     5041 ggtcgccacc gaaatcacga tggacatcag cgtctgccac ggcgccggcg tgtgggcgac
     5101 gatccagtct ttggcgagga cgaaaatctg atctgccgtg ccggtcgcac tcattgcggc
     5161 gtccccttcg gctcggcggg ctttgccgga gttgcggcta cattgccggt tgcttcgccc
     5221 gccggtggta ccgctgctgc cgctgctgcc gccttggcct ttgcagcggc ctcggccgct
     5281 gctgctttag ctttcgcttc ctcggcttcc tttgcgcgcc gttcgtccgt ctctgccgct
     5341 tccactggat gaatcttctg gtagtactcg ttcgacttcg ccagctcctg cttcgtgtac
     5401 agcagcccgc ggaaccggtc gtcggtgctc agctcataca ccttgtccat ccggatcgcg
     5461 tcaaacgggc acacctctac gcagatttgg caactcatgc agaccgatac atcaatattg
     5521 aagaccgcag gatacgcctg gggttttccc acgaaatccg gcttcttgtc cttgctcttc
     5581 tcaatgtgga tgcagcgtgg cgggcactcc ttctcgcaaa tctggcaggc gacgcaccgc
     5641 agccccgcgt ctggatcggc cccgtcgtag agcaggaacg gaaaattccg cgaagcctcc
     5701 ggcagcgtgt gccgctcctc cggatactgc accgtcgtca ggcgctcctt atcgacgtaa
     5761 ctgccgaaga agttccgcgc cgtctcgact aatccctgaa taatgccttc acccagcatg
     5821 gaaattccta aactctaaac tctcatccct aaactctcgc ttcaccggtc cacttctccc
     5881 agcacaatat ccacgctgcc cagaataatc accacatccg caatgttgtg ccccaggcac
     5941 atgtcttcca gaattgtcag gttgatcatg ctcggcggcc gcacacgata ccggtacgga
     6001 ttccccgaac catcgctgat caaaaagaat cccagctcgc ccttgggtga ctcgatccgc
     6061 ccgtatgcct cgcccgcttt cggccgcaac ccgcgcagac gtaccttcgg atccatgatc
     6121 ggaccttcgg gaatatcttt aagcgcctgc tccagaatct tcaccgtctc gcgcatttcg
     6181 agcacgcgca tcatgtagcg gtcgtagaca tcgccgtgct cgcccagcgg caccttgaaa
     6241 ctgaaacggt cgtagatccc atagtgatcc actttgcgga tgtcgtggtt cacgccgctc
     6301 gcgcgcagca tcggccccgt cacgctggcg ttgatcgcca gctcccgcgg cagcacaccc
     6361 gttccctgag cacgcgccat cagaatctcg ttcgcccgca gcagagcctc aaactcatcc
     6421 acaaatccgg gcaactgcgc caccagggcg cgtacctggt ccagccactc cttcgtcgca
     6481 tccacgcgca ggccgccaaa acgcatgtag ttgcacatca tgcgcgcgcc gctcagcgac
     6541 tcgaacaggt ccagtacttt ttcacgctcg cgaaaggcat acatcagcgg cgtgccgctt
     6601 gcgcccatgt cctgcagcag gaagccgatc aatccggcgt gattctggaa gcgcgtcagc
     6661 tccgcgacga tcacgcgcag atactcagcg cgctcgggca cctgcagccc ggccagcttc
     6721 tctaccgcca gcacgtagcc ccagttgttc gtcatcgagc acagatagtc cagacgatcg
     6781 gtgtagggaa ttgccgacag ataggcgccg tgttcggcaa tcttctcgtg gttgcgatgc
     6841 aggtagccga aaaccggctt cagacgcacc acgcgctcgc cctcgagctt cacgtccatg
     6901 cggaagacgc cgtgcgtcga cgggtgatgc ggccccatcg acacttccat gatttcgccc
     6961 tcttccggct cgtagatatc ccgatccagc agcgcgtccc tcatccgtgc gtctcctgtc
     7021 ccggagcggg cgtgcgtgcc tcgaccagcg gaatctcatc cgcaggcgcc acatagtcct
     7081 tccgcatcgg ataatcctta aacccgtccc acatcagaat ccgccgcaga tccggatgcc
     7141 cctcaaagac aatcccgtac aaatcaaagg cctcgcgctc ctgaaactcc gccccacgcc
     7201 acacgggggt cagcgacggc actcgcgtgt cgtccgtacg gtttcctgtg cgcagcctca
     7261 gcaccaccgg cccgtgcttt ttctccatcg agtagaggtg atacaccgcc tccagaaatc
     7321 ccggtacctt cgtcttccga acctcgtcca cttcctgctc cgactcggtg ccatcggcgc
     7381 ccttcaccag cttcttcact ttgacttttt ccgacgtctc cgtgtcgggc cagtccacac
     7441 ctgtcaccag cgtgcagtaa tccagccgcc actccgcgtg atcccgcagg aacttcgcca
     7501 ccccccccgc atgagcgtga tccagcagca gggagtgctg ccccgacgga cttccgttct
     7561 ggatgatggt caccgaagcg ccgggcacgc cggcctcgat ctgcgccttg atttcgtcag
     7621 gggagggaag cgccattact gaatcacctt caggtccgga tgggaaaccg gatatcccgg
     7681 cgcgtcgtgg aacacaaggt cgttctgcgg cggctccagc ccgtgctcgc caaactccgg
     7741 caccggaaac tcactgggca gatccttctg caggtgccgg ggtcgagccg atcccgccag
     7801 ggcctgctcc tcgatcttgt tctgcagcgt catgaacgcg tgaatcagcg cctcgggccg
     7861 cggcgggcat ccaggaatat gcacgtccac cggaatgtac cggtcgattc ccttcagcac
     7921 gttgtagcca tcgcggaacg gcccgcccga gatcgcgcac gcgcccatcg cgatgacgta
     7981 cttcggttcc ggcatctggt tgtagagccg aaccacctgc ggcgccatct ttttggtcac
     8041 cgtgcccgac acgataatca aatcggcctg ccgcggcgac ggccgaaaca cttccgcacc
     8101 gaaccgcgcg agatcgtagc gtgccatggt tgtcgcgatc atctcgatcg cgcagcacgc
     8161 gagcccaaac gtcagcggcc acacggagtt cttccgcccc cagttgtaca gctcctcgag
     8221 cgtcgtcgtg aagatcccgt tcttgctcag ctcgatcttc agttccttca gttcatccgc
     8281 catccgcagt tcctaaactc caacctctaa actctcgcgc gcctgaatct cgaatttcgc
     8341 tcgtcgtgcc gctcgtctct cgctcgcctt cggcgagcta tgcccacgtc aaaacaccct
     8401 tcccatatgc ccaaaccagc ccttcgacca gcagcagcaa aaacaccagc atcgccaggc
     8461 acgcgcccag cgaaagtcct gtgaatgcca ctgcaaacgg cagcaggaac accgcctcca
     8521 catcgaaaac cagaaaaaca atcgcgtaaa tgtaatagga cgcgccgaag cccatccagg
     8581 aatcgccctt cgactccagc ccgcactcat atgtggcgtt tttctcggcg cccggctttc
     8641 gcggcgaaaa gaacttcgcc cacagccagg ccagcgccag cggcgtaaat ccaaatgcca
     8701 cggcgcccac caggagcacg acaatcgtca gccagggatg ctcagcctgt cccatcatcg
     8761 acaatccact ctccggcctc gatcttcgac tcctgtgaga ctacctgtct caaaacacgc
     8821 cactccgtga ccccaatcac agattgctgc cttaccgttc gccttccggg gttcgccttt
     8881 ggctgcctct tcgcggccac tcgctgaact ctccatcctc gcacagttca gcctcaaccg
     8941 cagccctcga gccacggtag ggggaattcg agattgccga gcgaacccct tcgctccagc
     9001 agcttctgcc catcgcttct cgctaacagc ctttcgctaa acgcttctcg ccaaccgcta
     9061 tccgctgcct ttgccacctg tgttctctgt cacagccgac ccactcaccc acagcgcaaa
     9121 cttttctgtt gtggctttca ctcatcccgc actcgtaacc ctcgaacccc agcaaggaga
     9181 accatgagca ccccttctaa aaccgcgatc ttcgacggca acgaagccgc tgcctccgtt
     9241 gcctatcgct tctccgaagt cgttgcgatc tatcccatta cgccctcctc gcccatggcc
     9301 gagtttgctg accagtgggc cgccgacggc cagcgcaaca tcttcggctc ggtccctctc
     9361 gtagcggaaa tgcagagcga agggggcgcc gccggtgcta tgcacggagc cctccagggt
     9421 ggcgcctttg ccaccacgtt cacagcatcc cagggtctgc tgctcatgat ccccaacatg
     9481 tacaagatcg ccggcgaact caccccgacg gtcatgcacg tgaccgcgcg ctccgtcgcc
     9541 acccatgcgc tctccatttt tggcgatcac tccgacgtca tgggtgtccg ccagaccggc
     9601 tacgccatgc tcgcttccaa ctccgtgcag gaggccgccg atctcgccac catcgcacac
     9661 ctggccaccc ttgaagcccg cgtcccgttc ctccacttct tcgacggctt ccgcacttcg
     9721 cacgagatca acaaagttct ccccatctcc gacgacacca tccgcaacct gctcgacgaa
     9781 aaggccatcc gctcccatcg cgaacgcgct ctcacgcctg accggcccgt cctccgcggc
     9841 tccgcacaga atcccgatgt cttcttccag gcccgcgaag cctgcaatcc ctactacctc
     9901 gcctgcccgg tgattgtgca ggacgtgatg gaccgcttcg cccgtgaaac tggccgcagc
     9961 taccgcctct tcgactacta cggagccccc gacgccgacc gcgtcatcat ccttatgggg
    10021 tccgcgtccg gagccaccga ggaagccatc gacgccctca acgcccaggg cgaaaagctc
    10081 gggctcctca aagtccggct ctatcggccc ttcgccgctg aggccttcct cacagccctc
    10141 cccgaaaccg ttcgcaaaat tgccgttctc gatcgcacca aagagcccgg cgcgtcaggt
    10201 gagcctcttt atcaggacat cgtcaccacc atctccgaag cgctctcctc cgggcgcgtc
    10261 ccactctcgc agttcccggt catcatcggc ggccgctacg gtctctcctc caaggaattc
    10321 acgcctgcta tggccaaagg cgtcttcgac gagctcgcaa agccgcatcc caaaaaccac
    10381 ttcaccatcg gcatcgacga cgatgtgacc cactccagcc tgcactggga cgagaacttc
    10441 accaccgaaa gcgccaaaac cgtccgcgct ctgttctacg ggctcggttc cgacggcacg
    10501 gtaggcgcga acaaaaactc catcaagatc atcggctccg aaaccccgaa ctacgcccag
    10561 ggctacttcg tctacgattc caagaagtcc ggctctctca ccacctcgca cctgcgcttc
    10621 ggtcccgagc ccatccgctc gacctacctc atctctcgcg ccaatttcat tgcctgtcat
    10681 ggcttcgagt tcctcgaaaa gatcgatgtc ctcgccgccg ctgagcccaa cgccgtcttc
    10741 ctgctcaaca gtccctacgg ccccagcgag gtctggcagc atctgccccg gactatccag
    10801 aagaccatcc tcgagaagca catccgcttc tatgtcatcg atggattcac cgtcgcccgc
    10861 gaaaacggca tgggcacccg catcaacacc gtgatgcaga cctgtttctt cgccatcagc
    10921 ggcgttctgc cccgcgacga ggcgatcgat cagatcaaga aggccatcaa gaaaacctac
    10981 ggcaagcgcg gcgaagccgt tgtccagaag aacttcgccg ccgtagacgc ctcgctcgcc
    11041 caactgcacg aagtcaaggt tcccgaccac gtcaccgcga agttcgatat cctgcctgcg
    11101 attccaacgc aggctcccgc cttcgtccgc gatgtgctgg gtgctatggc cgccggcaaa
    11161 ggcgatctgc tgcccgtcag tgcgctgcct ccaggcggaa ccttccccac cggcacggcc
    11221 agatgggaga aacgcaacat cgcccaggtc atcccggtat gggaccagga cctctgcatc
    11281 cagtgcggca aatgcgtgat ggtctgcccg cacgccgtca tccgcgccag ggtctatgac
    11341 tcctcgctgc tgccgagcgc ccccgcaacc ttcaaatcgg cgaggccgaa gtggcgtggt
    11401 atggacagcg agctctatac acttcaggta tcacctgaag attgcacggg ctgccagctc
    11461 tgcgtcgaag tttgccctgc aaagagcaaa gccgacccgg cccacaaggc gctcaacatg
    11521 cagccccagc aaccgctccg cattcccgag cgcgcgaact gggagttctt cgaatccctg
    11581 cctctggtcg cccgcgaccg gctctccacc tcccaggtca aggacatcca gctcctcgag
    11641 ccgctcttcg agttctcagg ggcctgctcc ggctgcggtg aaacccccta catcaaactg
    11701 ctcacccagc tcttcggcga tcggctcatg atcgccaacg ccaccggctg ctcctcgatc
    11761 tacggcggca acctgcccac cacgccctac tgccagaact ccgaaggccg cggtcctgcc
    11821 tggtctaact cgctgtttga agacaacgcc gagttcggcc tgggtatgcg ccttgctgtc
    11881 gatcagcaga agacgtacgc ggaaggcctg gttgcaaaac tggcgcccag cctcggcgac
    11941 gacttcgtcc acgccctcct cgaagccgac cagacgaaag agcagggaat caacgcccag
    12001 cgcgagcgtg ttcgcgaact ccgcgccaaa ctcgccacca tcgactccga gccagcccgt
    12061 tctcttgatg cggttgccga caccctggtg cgcaaaagtg tgtggatcat cggcggcgac
    12121 ggatgggcct acgacatcgg tttcggcggc ctcgatcacg tcctcggttc cggcaagaac
    12181 gttaaagtat tggttctgga tactgaggtt tactccaaca ccggcggaca gatgtcaaag
    12241 tcaacgccac gcggagcagt ggccaagttt gctgctggcg gcaagcttac cggtaagaaa
    12301 gaccttgcca tggagatggt cgcttatggc tacgtttatg tggcccgagt agccctgggc
    12361 tcgaacgaca tgcatacggt caaggcgttt caggaggccg aggcatggga cggtccggcg
    12421 atcatcatcg cctatagcca ctgcatcgcc cacggctacg acctcaccca gggcctcgac
    12481 caccagaaag ccgccgttaa ctccggctac tggccactca tgcgttacaa ccccgctgtc
    12541 cgagcagtag gaaagaaccc cttcttcctc gactcgcgcg caccttcaat tccactcaag
    12601 gattacatct acaacgagtc gcgctacacg atgctccagc gtgctcatcc cgatgtcgcc
    12661 gaagaactgc tcaaagaagc gaccgacgac gtggagcgca cctggcgtgt gtactccggc
    12721 cgcgcttcca tgccgggtcg tgagtcttcg cccaacatct cgccgcgcaa cggtcacgag
    12781 cagccagcgg cgccaacgcc gcaaaaggaa gagtagccgc gggcgacaat cagcttttcg
    12841 tcccggaccc gttccgggcc ttctgccagc gccggtcgca aaccgccggc gctggcattt
    12901 tttgtggcct aagctgctgc tgagcacgtg cccattccag gttctcccag gcggcaaagt
    12961 gcttagcgac aaaaggcacg cttcccgcgc cccgccctgc tatcagaccg accgcgatcc
    13021 tccaggcccc gacggcggtc gccatcaatc gatgaaagat gccctcgtcg aattcttgca
    13081 gccgaagcct gtacagcatg gtggtaattg gcccggatag tccactttcg cgttagttcg
    13141 gatcgtacac agaatgtgta ttgaaatcgg tttcctaaca gtactaaggt gacgttacgt
    13201 cccgaaatga aacagaatcc actacggggc gagcctcgca tgcagcacct ccatcaacac
    13261 gatttctggc taccgatctt cagaaaaggt catgatgaac gacttgtctt ccttgccgtg
    13321 ccccccgagt ctctccaatc cggtctcttc tcccgtcctg tccaacattc agcgcaaggg
    13381 taaagcccac accaccacaa cctccaatcc caccagcggg cgtcagattc tgatcgttga
    13441 cgacgatgta tcgctggctg gtttcctgtg cagcgaactc cagggccaga agtacgccgt
    13501 tgatatcctc catgatggcg aggcggcctg cagtctcctc gatgagcagc gtcactacga
    13561 tttgctgatc ctggacctca acctgccgaa actggacggc atcgggctga ttgaacgcat
    13621 tcgtccggcg cgtccgcgaa cgcccatcct ggttctgacg gcgcgcagcc gggtcgaaga
    13681 caaggtcacc gctttccaga gcggggcgga tgactgcctg acaaagccat tctcgctggc
    13741 ggagctgttg gcgagggttc aggcgttgat gcgacgcaat tccggactgg taccgaactg
    13801 ctcgcaattg gccgacctga cgcttttacg cgaggaacgt cgtgtggagc gaaacgggcg
    13861 ccgcatcgat ctgacaccgc gcgagttcgc gatcctcgat gtgatgatgc ggaatgtcgg
    13921 acggcccgtc tctcgcgcca ccctgctcca ggaagtgtgg aacatgccgg gcgagccttc
    13981 caccaacatc gtcgacgtct acatgaagta cgtgcgcgat aaggtcgatg gtcccgggga
    14041 acggcgcctc acgcacacca tccgcgggtt cggatatgag ctgcgggatt ccgatgcata
    14101 gcaccccggt cagcggcaca agcgagacat ctgcggcaaa cgcatcttcg gcaaagttcc
    14161 accgcgatct cgcgaatgga ctgcacgcca tggcgcaacc gctgaccatg cttcgagcgt
    14221 cgatcgaggt gctggccatg ccgctgtcgg caggcatcga ccggcagaag tatctggaag
    14281 tgtctgccgg agccgtggag cgcacttgcc atttgttcgc gcgggtacag gacctggtcg
    14341 cagcttcgat gatcgaagca gagaaggcgc cttttgattt gtggagcatt gtcgccaatg
    14401 ttgtggaaga gcagagaaat cggctgcttg cctccggggc cacgatcgcc gcgacaaaga
    14461 ccgaaaattc gatgccgatt ctcggagacg ccgcgagaac cgagcaggcg attgttcttg
    14521 tgttgcggct ggcgggggaa ctggccaatg ccggcgatgt tattgaactt tccggatcgt
    14581 cgtccagagg gttcctcgaa ctgtgcatcg agaacagccg ccaacgcggc aagcccgtcg
    14641 attcgacggc gcgcctgaat ttggctttgg ccgaggcgaa tatcgcaagt cagcgaggac
    14701 gttatgaatt tgttgaggat ccgtttcgtg tctctctggc cttgcccctg gcggaccgcg
    14761 gtcaaccgga gatcgaaaca gacttgaatc gttgcgcacc tcaatcgatg cactaagcag
    14821 aggcttagtc atcttttgaa atcaccagca cagttcccgg cgaggttgcg ggacgcctgt
    14881 tatgaccagg cccaactgct tcgcgggtca tcttcacttg atacgttttt cggagaatgc
    14941 agtggcgact tctgtgttgt cttcgtcggt ttcggatcgt tttgaggatc gtctgcccat
    15001 cgatgtcttt gtggtcagct ccaacgcgcg gcttcgccag gatctgcaga ccaaactgac
    15061 ggctccccga tggcaggtct ctgaagccgg cagcggtgcg ggagccctcg aactcctccg
    15121 tcagcgcaat gctgacgaag cgatcctgtt gctggacccg gtgcttcccg atcttgagcc
    15181 cgatgacttc agtgccattg tgcgggaccg atatcccaac ctgcaggtgc tgatgctcaa
    15241 ttcgcacacc ggtcagctgc tggtcggcag catgtcgcca accggcgttt ccaccatgct
    15301 ggccgacgaa atcaacaaag gcggtgcggc tgcgagcaca gcggttcccg tgacgctgcc
    15361 ggacacgtcg cgcctgacca gcgatcacaa gccccgcttg cgcagtatga tcggcgattc
    15421 tgatgcaatg cttcgcacct tcaccatcac gcgcatggta gcggcacgga ataccaccgt
    15481 tctcgtcaca ggggaaagtg gtacaggcaa ggatttgctg gcacaggaag ttcacctcat
    15541 cagctcgcgg cgaaatcagc ctttcgtggt cgtgaactgc tcggccatcc ccgagcctct
    15601 gctggaagcg gagttattcg gatacaccaa gggatccttc accggagcgg tacaggcgcg
    15661 catcggccgg attcatgcag cccacggggg cacgctgttc ctcgatgaga tcggcgatat
    15721 gccgctttcg ctgcagagca agattttgcg cttcctcgag cagggtgaag tgcaacgtct
    15781 gggaggcaac gacaatctga aggtagacgt gcgagtcgtt gccgcaacca acgccgacct
    15841 gaagggcctg atcgccaaac agcagtttcg cgaagacctc tactaccgcc tgtctgtctt
    15901 tccgattcat ctcccgccgc tgcgtgagcg catggaggac ctggaacagc tcgcgcaggc
    15961 ctttgtggcg aagtttgcgc ccggcacgat gctgagccgt gcggcgctgc aaattctgga
    16021 gcagcatcgc tggccgggca acgtgcgtga attaagaaat gccatcgagc gggcggtcat
    16081 cctggcaggg atggaacggg aaatcaagcc cgaacacatt gttttgtagg actttcgttc
    16141 cgaatcggtg cgcctgttcc actttggcac tccaggtgca attgcaatag gcaaatgctg
    16201 cctggttctc atccggttcc aatgaacgag cccaacgttg cggtgctgca tagcccacag
    16261 ctgctggccg atgcgttctc ggaatttatc tcggcgtcgg ccacccttga agcgtcctat
    16321 cgcgacctgc aaagtgaagt ggcgcacctg agcatcgagc ttgctgagcg caatgccgag
    16381 ctgacccgca gcctcgaaga caacaatcga atgcgggcaa ccctgcagca gatgatcgac
    16441 tcgatgcctt gcggggttct ggtgctcgac gcagcggaga gcatcgtcac catgaatccg
    16501 gaaggacaca gactgcttgg gcggggaagc gcccgggcaa gaacgctgca cgacctgtca
    16561 gagatcagtc gtatcgattt cgaagccctg atcacggaga ccgagggaca gcgcgacaac
    16621 gaagtttgcc tcacagcagg gagcaaaaag cgctggctcg cgatcggccg ccgcgaactg
    16681 gatggcgcgc ccgcggcacg gttccagagc atctggatcc tgcgcgatat tactgcagac
    16741 aagctggcgg agcaggagcg ggaaagcgca cgccgggcga ccactttggc ggaaatttcc
    16801 accatcctcg cccacgaaat tagaaatccg ttagcgagtc tcgaactgtt cgccggactg
    16861 atcgaagaag atcgcggtgg caccgaagag tgggttgcca atctgcgggc gggtatccga
    16921 acgctctctg gaaccgtaaa caacgttctg agcatgaatg gcgacggcca caaacagctt
    16981 gcgccggtga acttgtcggc gtgcgcggcc agcggtgtag agtttgtcct gcccattgcg
    17041 cgtcaggccg gcgtgaagct ccagttttcg ccggcgcaga catccatcac ggttcgtggg
    17101 aatgaggatg cgctgcggca aatcctgctg aacctgatcg cgaatgcaat tcggcacaca
    17161 ccagtgggcg gcctggtcga agttgccgcg aaggccgaga cgcggcacgg agctatccgg
    17221 ggcatcgtta ccgtgcgtga caacggttgc ggtatccccc aggaccagct ggcacacatc
    17281 ttcgaggcgg gcttcagcgg taccggagag tcgccgggac tgggtcttgc cgtgtgtaag
    17341 cgcctcatgg ctcagcatca tggcgagatt cgcgttacga gccgtgtgga ccagggaaca
    17401 gagttcgaac tggagttttc agcaatatga caggtgagac cgtgtcctcg gtgatggtgg
    17461 tggacgatga gccgggcatc cgcactgcac tgcgtgccaa tttcttgcgg cacggctggc
    17521 gcgtggagac ggcctcgggc gtccgggagg ccatccgcaa tctggaaacc cgggaatacg
    17581 accttgtcgt taccgacatt cgcatgcccg acggtagtgg catggaagtg atgcgcgccg
    17641 cgcggaaaac ctcgtcctcg acggcggtga ttctgctcac cgcttatgga tcggttcccg
    17701 atgcggtcac ggccatgcgc gatggcgcgc tggattatct gaccaagccg atcccgttcg
    17761 accagctgca ggcgaccgcc gcgcaggtga tgcagcgagc gcagcaaatg aatcgaaacg
    17821 aaggtgtcgt cccaaccgtt gaacctgaaa ccggcaatgg gcaaatcgtc ggcggttcat
    17881 cgctcctcat gcgcgctttg caacgtgcgc gcgccgccgc gaacaccggc gccgacgtgc
    17941 tgatcgaagc cgagagcgga accggcaagg aactgctggc gcgcttcatt cacgatgcaa
    18001 gcgatcgcag ccagaagccg tttgtcgcag tcaattgcgc ggccgttccc gagacgttgc
    18061 tggagagcga actcttcggc cacggacgtg gtgcattcac aggcgcagtg gccagccggc
    18121 cgggcaaatt tgaatccgcc aacggcggca ccattcttct cgatgagatc ggcgagatgc
    18181 cgctcaacct gcagcccaag ctgttgcggg cactgcagga gcgcgagttc gagcgtctcg
    18241 gcgaaggccg cacggtaaaa gtcgacatcc gcgtcatcgc gacgaccaac gtctcgcttg
    18301 cggaaatggt cgagagaggc cagttccgcg ccgatctcta ctaccgcctc aatgtgattc
    18361 cgctgagcct gccgccgctg cgcgagcgtc gcgacgacat tccgatgctg gcgcggcatt
    18421 tcgcggaaca gttcgcgggt accactggcg ccctccgtcc ccgcctgcat ccggatttca
    18481 tcgagcgcct gcagtcacac agctggcccg gcaatatccg cgaactggcg aacttcatgc
    18541 gacgcgtcat taccctcagc ggcacgctcg agatcgatgc gcgctgcttc gagatggagt
    18601 ttcagggaat cagcgcgcca cagcgcaccg ttcccgccaa aactgcgagc atcccggcgc
    18661 cggccggcac acccatccgc gaactggaaa gaatgcacct cgaaaacact cttgccctga
    18721 ccgacggcaa tcggacccat gcagcggcca tgctcggcat cagcctgcgc acgctgcgca
    18781 acaagattcg cgaatatggt ctccctccaa ggaggtacgc atagatggca gacgtttcaa
    18841 tgctggaaat gctctcgggc tatctcaaaa taacgaccga tcgccagcag gtgatcgcgt
    18901 cgaacatggc caatgtcgac acgcccggct accacacgcg cgacgtcaac ttccgtcagg
    18961 cgatgcagga ggtgatgaat cctggtggcg agcccgggat ggaaacgacc agcctcgaga
    19021 ttcagggcct gccggagcgg gcggacggca ataacgtaaa catcgaccgc gagagcatgc
    19081 tgctctccga gacccagctg cagtaccaga tgggcgtcca gctcattaag gagcggttcc
    19141 atacgcttct gacggcgatc aaggattagg gaggcaaacc atgggcttct gggacatgat
    19201 gagcatcagt gcttcggcgc tcgcggcaga aagacagcgc gctgaagtga catccgcgaa
    19261 cctggcgaac gcacagacga cgcacaccga aggcggcagc ggcccctacg ttcgcaagga
    19321 ggtcgtgttc agcgcgacca atgcctcgcc cttccagacg atcttcaacc gcgctggaca
    19381 ttttggagcg cctgcaggct cggtggaact gagcggtgtg gtcgaagaca gcgcgccgcc
    19441 ggtgatgcgg tacgagccgg gcaatcccga cgccaacaaa gaaggctatg tggcctatcc
    19501 cgcgatcaat ccgatcacgg aaatggtgga tctgatggac gcgacacgcg cctatcagct
    19561 gaacgcatcc gcggtcacct cagccaagca aatggttcag gaatcgatcg acattctgaa
    19621 aacgacataa cgacggagca gcacgatgag caacattagt ctcggtggta ttcagggcgc
    19681 ggtc
//
LOCUS       DASZYN010000195         1744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_264819, whole genome shotgun sequence.
ACCESSION   DASZYN010000195 DASZYN010000000
VERSION     DASZYN010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264819"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..544)
                     /locus_tag="VHE33_05000"
     CDS             complement(197..544)
                     /locus_tag="VHE33_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVT96843.1"
                     /translation="MKIGTIIRGYRLQKGLSQGDIEKRTGLLRCYLSRVENGHTVPSL
                     DTLAKIAGALELPLAQLFAEDTLGRQLQSQKLSDDELRFLTQIRRYSTNLNDSDRKLL
                     LAMVKKFAATATR"
     gene            1062..1553
                     /locus_tag="VHE33_05005"
     CDS             1062..1553
                     /locus_tag="VHE33_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96844.1"
                     /translation="MTQRFFRISILLALAFAASQTLLSAQERSSLDGVWAVTVTVTNC
                     TTGAPIRTVASLQQFHPDGSMIETANTASRGISEGVWNPVGRHTVAAFYWFYRYNPDG
                     TFASRATITDKIVLGSQGQFTSNGMVQDYDATGALLTTGCFTHSATRLATSVGPHGDD
                     WNH"
BASE COUNT          328 a          646 c          424 g          346 t
ORIGIN      
        1 ctcgtccccc gcatcggagc accacgagaa acattcctgc gccggcgcgc catcgcagtg
       61 ggcgtcatgc tctttgtcct gctgttgatc gcgtggggtc tgcgcgcgtt ctacgaaggt
      121 ccggcagggt agaacggtcg aagggctcca ggattggccc ggagccgcga gtggaaatct
      181 tcccaaaacg aatgaactac cgcgtcgcgg tggcagcgaa cttcttcacc atagcgagga
      241 gcaacttgcg atcgctgtcg ttcaggttgg tcgaataccg ccggatctgc gtcaggaagc
      301 gaagctcatc gtcggaaagc ttctgtgact gcagctgccg tcccaaagta tcttcggcaa
      361 aaagctgagc gagcggaagt tccagcgccc cggcaatttt ggccagagtg tcaagcgaag
      421 gaaccgtatg cccgttttcg acgcgggaca aataacaacg gagaagaccg gtccgcttct
      481 caatgtcccc ctgcgagagc cccttttgca gccgatagcc ccggataatc gttccaattt
      541 tcatagtggt gggtgtacaa acgcactttc ccctgtgaac agcaaggcag gaattctgtg
      601 atattgcctg catagttaac acgttcgggg aatcacaagc aagcaatatt tttccgcctt
      661 tagtaacctc ggcaggaacc cggggatgtt accacccgcc gcgaatcaca cgtttttcaa
      721 agacttacgg aagcaaaaag ccggacgccc cgtccctccc tcccacgctt cccatccccg
      781 cccggaaccg gatccccgca acgattgcaa ccgagctgct ccctccggtt tgtgacgttc
      841 tcccctcact gcagtcaccg cgcccgccgg cgagatcggt ttcccggcac tccccgtcgc
      901 gtccctcaaa cccaatctgg cccctctcca ggaaactttt cctccccctg ccagtttttc
      961 cgctgctttt gcgcctgaag ggcagcgatc gcagtgaccg gatcccctga ctcgccggcc
     1021 ctccgctccg caacccagtt cacacccaaa ggagatttgc catgactcag cgcttcttca
     1081 gaatctcgat ccttctcgcc ctcgccttcg cggcctcgca gacccttctc tccgctcagg
     1141 agagaagctc cctcgatggc gtctgggccg tcaccgttac cgtcaccaac tgcaccaccg
     1201 gcgcgcccat ccgcaccgtc gcctcgcttc agcaattcca ccccgacggt tccatgatcg
     1261 agaccgccaa caccgcctcc cgcggcatca gcgaaggcgt ctggaacccc gtcggccgcc
     1321 acaccgtcgc cgccttctac tggttctacc gctacaaccc cgatggaacc ttcgcctccc
     1381 gcgccaccat caccgacaaa atcgtcctcg gctcccaggg ccagttcacg tccaacggca
     1441 tggtccagga ctacgacgcc accggcgccc tgctcaccac cggctgcttc acccactccg
     1501 ccacccgcct cgccaccagc gtcggccccc acggcgacga ctggaaccac tgagccttca
     1561 ctcccagggc cggctgcgat caacccatcc acgaccggcc cagggatcgc cccgcgcttt
     1621 ctcacttgaa tccctccctg tacgcgatta cattgcgggt ggtggtgatc gacatgcctt
     1681 cctctgctct gccgcggctc tcccgcaagt tttccctctc tctcctcctc atcctcctca
     1741 ccgc
//
LOCUS       DASZYN010000196         5175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_907295, whole genome shotgun sequence.
ACCESSION   DASZYN010000196 DASZYN010000000
VERSION     DASZYN010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907295"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VHE33_05010"
     CDS             complement(<1..326)
                     /locus_tag="VHE33_05010"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HVT96845.1"
                     /translation="MKWFYDMKIGTKLIASFVVVAAISAVVGWIGIANMAKMSDLADS
                     TYANETMGLLYLKQANVWVIYVSRAEKNVMLASTAEERDRYMANVREYEGKVEASIDQ
                     ARPLIRS"
     gene            753..2462
                     /locus_tag="VHE33_05015"
     CDS             753..2462
                     /locus_tag="VHE33_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96846.1"
                     /translation="MKTSALFCSLTVSVVAAAFLVPAGIAQTAPSASGSVVKTSKPAR
                     PGAGVSSQDVDALRRAIEQQQTEQQQQLDQLRQQNDVLAAQLRAAEEQLSRTQHQIDQ
                     LGAASQPQITDLQAAVAALKTAQSASTAWVDQQKKLEPARENPTGLRYRGLTLVPGGY
                     FSADGLFRSHAENADINTSFAAIPWDAAPMSHQSEFRATARATRLTLRASGAAGRATV
                     TGYFETDFLGSGFAASEVQTNGYSNRVRQMWGRVQFPNGWTFAGGQMWSLITMNRTGI
                     ENLTEYAPGMIDATILPGYDYARQTSFRVTRRFTASKSTVAFSLENAATVGNTPANVP
                     SSIAGILSGLSTTGTGVLSNTTYSTNVAPDLIGKIAFDPRLGHFEIKAVGRTFRDRLN
                     STATVPGKNNTLPGGAIGASAYLPVGTPKVSFLAQAMYGAIGRYGATSTDVIVKPDGS
                     LSAEKSIHGITGFEYRPSPRMDTYLYGGEEYLPRNYGYGLKTIDNSKCFAEAGFSCSA
                     GLKVLDSAATGFWYRFYKGPGGTLQYGASYIYILKEAWSGVGGAPHGVDNIVESSFRY
                     YIP"
     gene            2789..3559
                     /locus_tag="VHE33_05020"
     CDS             2789..3559
                     /locus_tag="VHE33_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96847.1"
                     /translation="MRFSVATLRPLLLTASAAALAISALAQPIPAATAIPIVLTKTIT
                     AGQAHPGDPIAARTLQAVLLPGARLLPAGTLLTGHIVTASPFHFDATPYAAQQPSTLS
                     IHFDAIEHGSATPVSLSLRAIAGSVASHEAQVPHLLDETDGSNTRELIGGETASPLDK
                     TIQAPDGTIVGYNRTQGLFAHLLAAASPADPNLTCNATSTEQSLGLFSADACGVYGLN
                     AVSLANNGVTDGTFLLESRQRSVQLDAGTTALLEVLSR"
     gene            3827..>5175
                     /locus_tag="VHE33_05025"
     CDS             3827..>5175
                     /locus_tag="VHE33_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96848.1"
                     /translation="MTTRPARTPVLILTVLSLGCAPFHAQGKSKSGSTATAGSSPGSS
                     SSTSASPAAADRPFSIETEMFTYKAVEHNSAVIACDTAQYLFNGQVSDAPTGSHAPCI
                     ISGASARPGVVLISSGSTLLSDFQNWRADMSSMQGLVAHANQTCVANPEKAGSPEQPG
                     QPPAQQPPQQHHMRSRGLLGSALGSLADPTEAASIAGQVMGMFSSNQSVTSVIGTVQD
                     PALMNEVARQLRALNVQVLIPELYNPYSLGAIDSAHSPYMQNMQSVFDAYDKCSEDKS
                     GYPATSAQAADIDAVLTSIQSFLKEAFASAPRASTAATTAAPVDPGAQTEGTPSSPPS
                     HFASVIAADALARKIGFSASGAPDPNSPWHDLLWVKALESGGSVTKQSNLFGTKVLFG
                     GGAVDTYSVFNLEGDVVCAGNVYSFQQPVNVKDVQKAVNVPSPDPPAKWMSEHSTCAP
                     "
BASE COUNT          934 a         2018 c         1253 g          970 t
ORIGIN      
        1 gagcggatca gtggccgcgc ctggtcgatc gacgcttcca cctttccctc atactcccgg
       61 acgttggcca tgtatcggtc gcgctcctct gcagtcgagg ccagcatcac gttcttctcg
      121 gcgcgcgata cataaattac ccacacgttc gcctgcttca gataaagcag tcccatcgtc
      181 tcgttcgcat aggtcgaatc ggccagatcg gacatctttg ccatgttggc gatgccgatc
      241 cagcccacca cggccgaaat ggcggccacg acgacaaaac tcgcaatcag cttcgtgcca
      301 atcttcatgt catagaacca cttcatctgc tttctccccg ttcatcgcgc aaccatgcac
      361 tgcgcagagc gccgcattcg ccggcattat cctcgccgga gttcttccag attgatcgcc
      421 acatcccgaa ctgtctttac tgaatttctt tctcactgca ccgcagagct gcttttgttc
      481 cggattggaa ctttcccttt ccggccctca ccgtcgaagg gtgccgtctc cctgccggac
      541 gaacaaccgg ttcctgtcgc acgcggattc ggtggcgcaa atagctcttc cccgttatcg
      601 gctctgcgct gcgccggatg aggaaacgca tcccggccga ggccagccaa tcccatttcg
      661 gaaacaactc aaccgaagct gcgcgggaat cctcaccctc cctcctccgc cgccgatacc
      721 ttcagcaacc gccgttctcg gcggagttgc ctatgaagac ctctgctctg ttttgttctc
      781 tgaccgtctc cgtcgtcgcc gctgccttcc tggttccggc cggcatcgca caaaccgccc
      841 cttcggcttc cggctctgtt gtgaaaacct cgaagccggc caggccagga gccggcgtct
      901 cttcgcagga tgtcgatgcg ttgcgtcggg cgatcgaaca gcagcagacc gagcagcagc
      961 aacagctcga ccagctccgg caacagaacg acgtgctcgc tgcgcagctc cgtgccgccg
     1021 aggaacagtt gtcccgcaca cagcaccaga tcgaccagct cggcgccgcg tcgcagcctc
     1081 aaatcacaga tctccaggct gcggtcgccg ctctcaaaac cgcgcagagc gccagcactg
     1141 cctgggtcga tcagcagaaa aagctcgaac cggcccgcga aaaccccacg ggcctccgct
     1201 accgcggcct gacccttgtg ccgggcggct acttctccgc cgatgggctc ttccgcagcc
     1261 acgctgaaaa tgccgacatc aataccagtt ttgccgcgat cccgtgggac gcggcgccga
     1321 tgtcgcatca gagcgagttc cgcgccacgg ctcgagccac ccggcttacc ctgcgcgcct
     1381 ccggcgctgc gggccgtgcg accgtcaccg gctatttcga aaccgatttc cttggcagcg
     1441 gatttgcagc cagcgaagtt cagaccaacg gttacagcaa tcgcgtccgc cagatgtggg
     1501 gccgtgtgca gttccccaat ggctggacct tcgccggagg ccagatgtgg tccctgatca
     1561 cgatgaaccg caccggcatc gaaaacctca ccgaatacgc ccccggcatg attgacgcga
     1621 ccattcttcc cggctatgac tatgcccgcc agaccagctt ccgcgttacc cgcaggttta
     1681 ccgcctccaa atccacggtc gccttctccc ttgaaaacgc ggccacggtt ggcaacacgc
     1741 ccgccaatgt tccctcttcg atcgctggca tcctctccgg cctgtccacc accggaaccg
     1801 gcgtactcag caacaccacc tactccacca acgttgcccc ggacctgatc ggcaaaatcg
     1861 ccttcgatcc caggctcggt cacttcgaaa tcaaagccgt gggccgcacc ttccgcgatc
     1921 gccttaactc caccgcgacc gtccccggca agaacaacac gcttcccggc ggcgcgatcg
     1981 gtgcttcagc ctatctcccc gtcggcactc ccaaagtcag cttcctcgct caggccatgt
     2041 acggcgccat cggccgctac ggcgcaacct ccaccgatgt catcgtcaaa cccgacggct
     2101 ccctctccgc agagaagtcc atccatggca tcaccgggtt tgaatatcgt ccgtcccccc
     2161 gcatggatac ttacctttac ggtggagagg aataccttcc ccgtaactac ggctacggcc
     2221 tcaaaaccat cgataactcg aaatgcttcg ccgaagccgg cttctcctgc tcggccggcc
     2281 tcaaagttct cgactcagcg gccactggct tctggtaccg cttctacaaa ggtccaggcg
     2341 gcaccctcca atacggagcc agctatatct atatcctcaa agaagcctgg tccggcgtgg
     2401 gcggtgctcc ccacggcgtc gacaacatcg tcgaatcatc cttccgctac tacattccct
     2461 gatcgatctg gcttgcaatt cctggcctcc ggctgaactg gccggaggcc tttttctctg
     2521 cgctgccgat cgcctgctgc ctgttccatg ctccctgttg cctgctcccg gttgctgttg
     2581 cctaattgcc tgctgcctgt tgttctgtct cacttcggga caggtaacgc cccctcagta
     2641 cttcctggga attgcaggcg acgtttccgg caatagaatc ggcagctaga cacccggctt
     2701 gaacgccctg tgtcaaaatc gcgccctgcc ggagctcccg agccttctcg attcgctccc
     2761 gccgcgtcga atggaaagga tcgtccccat gcgcttctca gttgcaaccc tgcgccctct
     2821 cctcctcacc gcatccgctg ccgctctcgc catctccgct ctcgcgcaac ccatccctgc
     2881 cgcaaccgcg attcctatcg tcctcacgaa aaccatcacc gccggacagg cccatcccgg
     2941 cgacccgatc gccgcccgga ccctgcaggc cgttctcctc cccggcgcgc gcctccttcc
     3001 cgccggaact ctgctgaccg gccacatcgt caccgcatct ccattccact tcgacgccac
     3061 cccttacgcc gcgcaacagc cttccaccct gtccatccac ttcgacgcca tcgagcacgg
     3121 ctccgccacc cccgtcagcc tctcccttcg cgccatcgcc ggatccgtcg cctcccatga
     3181 agctcaggtc ccgcatcttc tcgatgagac cgacgggtcc aatacgcgcg aactcattgg
     3241 cggcgaaacc gcctcgcccc tcgacaaaac catccaggct cccgatggaa ccatcgtcgg
     3301 ctacaaccgc acccagggcc tctttgccca ccttcttgcc gcggccagcc cggcggatcc
     3361 gaacctcacc tgcaacgcca ccagcaccga gcagtccctc ggccttttct ccgccgatgc
     3421 ctgcggagtc tatggcctca acgcggtctc cctcgcgaac aacggcgtca ccgacggcac
     3481 gttcctcctc gaatcccgcc agcgctccgt ccagctcgac gccggcacca cagcactcct
     3541 cgaggtcctc tctcgctgac gtattccggc tttcccggta gcgctttccg ctgaccgctt
     3601 tttcgctaac cggttcttag ccaaccgctt catcgctaac cggttgaccg ctaaccggtt
     3661 catagctggc cgcttttcag ctaaccagtc cttacctccg ccgtggtatg ctcccgtcac
     3721 acaggccccc tggcgaccca tgcccggggg aagggcgaat cccccgcttg ccctgtctcg
     3781 cgccccaggt cccaccccag ggaaaccgcg caggaggatc gtccctatga ccacacgccc
     3841 ggctcgcact ccggtgctca tcctcaccgt gctcagtctc ggctgcgccc ccttccacgc
     3901 ccagggcaaa agcaagagcg gaagcaccgc aaccgccggc tcctccccgg gcagcagttc
     3961 ctccaccagc gcttcccccg ccgcggccga ccgccccttc tccatcgaaa ccgaaatgtt
     4021 cacctacaag gccgtcgaac acaacagcgc cgtcatcgcc tgcgacaccg cgcagtatct
     4081 cttcaatggc caggtctccg atgcgcccac aggttcgcac gccccatgca tcatctccgg
     4141 cgcgtccgcc cgccccggcg tcgtgctcat ttcctccggc agcaccctgc tctccgactt
     4201 ccagaactgg cgcgccgaca tgtccagcat gcagggcctg gtcgctcatg cgaatcaaac
     4261 ctgcgtcgct aaccccgaaa aggccggtag cccggaacag ccgggccagc cgccggcaca
     4321 gcagccgccg cagcaacacc acatgcgttc caggggattg ctcggcagcg cgctcggcag
     4381 tctggccgac cccaccgagg ccgcatccat cgccggtcag gtcatgggaa tgttcagcag
     4441 caatcagtcc gtcacgtccg ttatcggcac cgttcaggat cccgcgctca tgaacgaagt
     4501 cgcgcgccag ctgcgcgcgc tcaacgtcca ggtcctcatc cccgagctct acaatcccta
     4561 ctccctcggc gccatcgact ccgcccactc gccctacatg cagaacatgc agagcgtctt
     4621 cgacgcctac gacaagtgca gcgaagacaa atccggatac cccgcaacct cggcccaggc
     4681 cgccgacatc gatgccgtgc tcaccagcat ccagtccttc ctcaaagaag ccttcgcctc
     4741 cgctcctcga gcctcaacgg ccgccaccac cgctgctccc gtcgatcccg gcgcacagac
     4801 cgagggcact cccagcagcc cgccctccca cttcgcctcc gtcatcgctg ccgacgccct
     4861 cgcaagaaaa atcggcttct ccgcgtccgg cgctcccgat cccaacagcc cctggcacga
     4921 tctgctctgg gtcaaggcgc tcgagtccgg cggctccgtc accaaacaga gcaacctctt
     4981 cggcaccaaa gtcctcttcg gcggcggcgc cgtcgacacc tactccgtct tcaacctcga
     5041 aggcgacgtc gtctgcgccg gcaatgtcta cagcttccag cagccggtca acgtcaaaga
     5101 tgttcagaaa gccgtcaacg tccccagccc cgacccgccc gccaaatgga tgtccgagca
     5161 cagcacctgc gcccc
//
LOCUS       DASZYN010000197         6297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_584235, whole genome shotgun sequence.
ACCESSION   DASZYN010000197 DASZYN010000000
VERSION     DASZYN010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584235"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2267
                     /locus_tag="VHE33_05030"
     CDS             <1..2267
                     /locus_tag="VHE33_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT96849.1"
                     /translation="MNTSPDGNFGVASPTKFNNWRAQTSVLQDVSAYDFGGPGFNLTG
                     EVPEQVHGIHVSEAYFRLFGAPVILGRTFTPQEDSPNGGHVVVISYGLWQRKFGGNPK
                     IVGTALSLGNEPYTILGVIGQQFPSDPPADIWLPFQIDPNSTNQGHFFLAAARLKPGV
                     TLAQANAQMKLAYEEFRRRWTLADPKASFGVQPLRDSIVAGARSSLLVLLGAVGFVLL
                     IACANVANLLLVRASGRKREFAIRAAMGAGRGRIVRQLLTESVVLALTGGILGLIVGY
                     VGVRALFTIGTAQLPLVGKNGAAVGLDWRVLLFTLGISLLTGILFGLAPAIGASRPDL
                     NTTLKESSNRSGTGLRHNITRSLLVISEVSLALVLLIGAALLIRTFIALRHVDPGFDA
                     RNVLTMEMSLTGDRFQKTAAVAQLTLEARQRIDVVPGVEAAASTCCLPLEGGFGLPFD
                     IVGRPQGKDPNTGGAGWMNTSPGYFATFHIPLRGRDFTDQDSGSAPLVVIINETMAKK
                     WWPKENPIGQQIVIGKGVGPQFAEGPRQIIGVAGDIRDGGLNRDPRPLMIVPQAQVTD
                     GVTALNAGIGPVAWIVRTHGDPHQYISSVTEQLRQASGGFPVARVRPMEEVVSNSTAR
                     EDFNMMLLTIFGASALILAAIGIYGLMAYSVQQRTQEIGIRMALGADRSRIRSLVVWH
                     GMRLAIVGVVIGIGAAFGLTRFIASFLFGVKTWDPFVFVTVPIILSAVALLAVWMPAS
                     RASRLDPQQALRIE"
     gene            2264..3625
                     /locus_tag="VHE33_05035"
     CDS             2264..3625
                     /locus_tag="VHE33_05035"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVT96850.1"
                     /translation="MTAHRKCLPLVLVFCTLPLVAQQTGLDASIDAYVKPYVDTNNFS
                     GAILVERDGKVLLQKAYGFADREHRVDNTLKTRFHVASISMQFTAAAVLRLADRRQLS
                     LDDTIGDVLPGIPGANRITIRDLLTERSGLPDINSFADYNEVLQKHQTPASLVAKING
                     KPLLFPPGSQFLHEEHSAYNLLALIVEQKTGLPFAAAMHNLVFQPATLNGSFIDDDSP
                     LDITDRAIGYQPAGVSDLQPATQIHWSAKSGNASVCTTVGDEARWVDALFNGSLLTAA
                     SRTAVLENPQNIGYGWFRANKERFSETAYYMNGRAPGFASFVLYLPRERLTVVAFSNI
                     YSSATTTIGYDIAAIALALPYDTFTAASPLTSAAIAGSTGTFQFGPDFYQRNAKLELI
                     SASGGLALHWPSGDLSPLIPQSSDHFIDRSYWENVSIERDAAGHAVALNYDRFRGPVV
                     PTP"
     gene            complement(3659..>6297)
                     /locus_tag="VHE33_05040"
     CDS             complement(3659..>6297)
                     /locus_tag="VHE33_05040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT96851.1"
                     /translation="FGNFWRKLKLLFGRETYAGELEEEMAFHREQMAKDLQESGMTPE
                     AARFAAARQFGNATRIREQSHEAVGFRAETIAQDLRFALRQLRKSPGFAVTAILMLAL
                     GIGASSAIFGFVDAALIQPLPYAQQNRLVDVDESAAAFPRSNISRDDYEDWKRLNTTL
                     QSIDVYTGSGFLLRRGSSAEPVPAARVSDGFFHTLGVQPILGRDFRPGEDKPGGAKIA
                     ILSYGTWVTRFGGRRDVIGEKISLSGDPFTIIGVLPRDFAFAPRGGAELWVPLLDKNG
                     CEQRRSCHNLDGVGRLRDGVTIEQARADLQKIAAQLAIQFPTSNTGQGASVQPLSEFI
                     VGKVRPILLTLLAGAGLLLLIACVNVASLLLVRSESRRREIAVRGALGATPARLLRQF
                     ITEALLLAIAGCGAGVLAAAGMMSLLRSLFPKQIAQGAPFLQNVGLNLHTWAFAATVA
                     LLALLLMALTPAMRLAVRDIHEALSEGGRTAAGRFWRKLGANLVVVELTIAMVLLAGA
                     GLLGKSLYRLLHVDLGFDPTHLATVQLMMTDPASQKPEELRALYREIDTRVGGLPGVE
                     SLGLSSDLPVQCYCDTDWIRIPGQPFHGEHNDVMERDISSDYMRTLGAKLVEGRLFTA
                     QDDENHPRTTVINQTLARKYFPGEDAVGKMIGDIKLSPKSMRQVVGVVADVREAALDN
                     DPLPTEYFSISSGPDAFFALAVRVRGDDKAFLPELVKTLRAIHPALGVYGEITMEQQI
                     EQSPTAVMHQLSAYLVGGFAVLALVLGVVGLYGVVAYSVSQRTREIGVRMALGAQRGA
                     VHRLILKEAGLLAAIGIAVGLGLSIGASAAMKSLLFGVRAWDLTTLGGVAVVLGAFAL
                     LASFIPARRAARVNPVEALRAE"
BASE COUNT         1171 a         2312 c         1651 g         1163 t
ORIGIN      
        1 tgatgaacac atcgccggac ggcaacttcg gcgtcgcttc cccgaccaaa ttcaacaact
       61 ggcgcgcgca aaccagcgtt ctgcaggatg tctccgcgta tgacttcggc ggtcccggct
      121 tcaatctcac cggcgaagtc cccgagcagg tgcacggcat tcatgtttca gaagcgtact
      181 tccgcctctt tggcgccccc gtgatcctcg ggcgcacctt cactccgcag gaagactcgc
      241 ccaacggcgg gcacgtcgtc gtcatcagct acggcctctg gcagcgcaag ttcggcggca
      301 atccgaaaat cgtcggcact gccctgtctc tcggcaatga gccctacacg atcctcggcg
      361 tcatcggcca gcagtttccc tccgatcccc ccgccgacat ctggctgccc ttccagattg
      421 accccaacag caccaatcag ggtcacttct tcctggcagc ggcgcggttg aagcctgggg
      481 tgacgctcgc gcaggccaac gcgcaaatga agcttgccta cgaggagttc cgccgccgct
      541 ggactctggc cgatcccaag gccagcttcg gtgtgcagcc tctgcgtgac tccatcgtcg
      601 ctggcgctcg ttcttcgctg ctggttctgc tcggagccgt cggcttcgtg ctgttgatcg
      661 cctgcgcaaa cgtggccaac ctgctgctgg tccgcgccag cgggcgcaag cgggaatttg
      721 ccattcgcgc cgcaatgggc gccggtcgtg gccgcatcgt ccgccagctg ctcaccgaaa
      781 gcgttgtgct cgcgctcacg ggcggcattc tgggtttgat cgtcggctac gtcggcgtgc
      841 gcgccctgtt cactataggc acggcccagt tgccgctcgt cggcaaaaat ggcgccgccg
      901 tcgggctcga ctggcgcgtg cttctcttca cccttggcat ctcgctgctc accgggattc
      961 tctttggact cgcccccgcc atcggcgcct cgcgccccga cctgaacacg acgctcaagg
     1021 aaagcagcaa ccgctccggc accggcttac gccacaacat cacccgctcg ctcctggtca
     1081 tcagtgaagt ctctctcgcg ctcgtgctcc tgatcggagc cgccctgctc atccgcacgt
     1141 tcatcgcgct gcgccacgtc gacccgggct ttgacgcccg caacgtgctc accatggaga
     1201 tgtccctcac cggggaccgc ttccagaaga ccgctgccgt tgcgcagctc actcttgaag
     1261 ctcgccagcg catcgatgtc gtacctggcg tcgaagccgc cgcatccacc tgctgtctcc
     1321 ccctggaagg cggtttcggc ctgcccttcg acatcgtcgg ccgcccgcag ggcaaagacc
     1381 ctaacacggg cggagccgga tggatgaaca cctcgcccgg ctacttcgcc accttccaca
     1441 tccccctgcg cggccgcgac ttcaccgacc aggacagcgg ctcagcgcct ctggtcgtca
     1501 tcatcaacga aaccatggcg aaaaagtggt ggccgaaaga gaaccccatc ggccagcaaa
     1561 tcgtcatcgg caaaggtgtc ggcccgcagt tcgccgaagg ccctcgccag atcatcggcg
     1621 tcgccggcga cattcgtgac ggcggcctca accgcgatcc ccgcccgctg atgatcgtgc
     1681 cccaggccca ggtcaccgat ggcgtgacgg cgctgaacgc cggcatcggt ccggtcgcat
     1741 ggatcgtgcg cacgcacggc gacccgcatc aatatatatc ctccgtgaca gaacaactcc
     1801 gccaggccag cggtggattc cctgtcgccc gcgtgcgccc catggaagaa gtcgtctcca
     1861 actccaccgc gcgcgaggac ttcaacatga tgcttctgac catcttcggc gcatccgctc
     1921 tgattctcgc ggccatcggc atctacggcc tcatggccta ttcggtgcag cagcgcactc
     1981 aggagatcgg cattcgcatg gcgctcggtg ccgaccgctc ccgcatccgg tcgctggtcg
     2041 tctggcacgg gatgcgcctg gccattgtgg gagtcgtcat tggtatcggt gcggccttcg
     2101 gcctcacccg cttcatcgcc agcttcctct tcggcgtcaa aacctgggat cccttcgtat
     2161 ttgtaaccgt gccgatcatc ttatcggcgg tggcactgct cgctgtctgg atgccggcct
     2221 cgcgggcatc gcggctcgat ccccagcagg cgctaaggat cgaatgacag cgcaccgtaa
     2281 gtgccttccg ctggttctcg tcttctgcac gctgcctctc gtcgcacagc agacgggcct
     2341 cgacgcatcg atcgatgcct acgtcaaacc ctatgtcgac accaacaact tctccggcgc
     2401 gatcctcgtc gaacgcgacg gcaaagttct cctgcagaag gcatatggct ttgccgatcg
     2461 cgaacaccgc gtcgacaata cgctgaagac gcgctttcac gtcgcgtcca tttccatgca
     2521 gttcaccgcc gcggccgtgc tgcgccttgc cgaccgccgc cagctttcgc ttgacgacac
     2581 catcggcgac gtcctgcccg gcattcctgg tgcgaatcgc atcacgatcc gcgatctgct
     2641 caccgaacgc tccggcctgc ccgacatcaa ctccttcgcc gactataacg aggtgctgca
     2701 aaagcatcag accccagcct ccctcgtcgc aaaaatcaac ggcaagccgc tgctcttccc
     2761 acccggttcg cagtttttgc acgaggaaca ctccgcctac aatctgctcg cgctcatcgt
     2821 cgaacaaaag accggccttc cctttgcggc cgccatgcac aacctcgtct tccagcccgc
     2881 aacgctcaac ggctctttta tcgacgacga ctctccgctc gacatcaccg acagggcaat
     2941 cggctaccaa ccggccggcg tctccgatct ccaaccggca acccagatcc actggtcggc
     3001 caaatccggc aacgcctccg tctgcaccac cgtcggcgac gaggcacgct gggtggatgc
     3061 cctcttcaac ggctccctgc tcaccgccgc ttcccgcacc gccgtactcg agaacccgca
     3121 gaatatcggc tatggctggt tcagagcgaa caaagaacgc ttcagcgaaa ccgcgtatta
     3181 catgaatgga cgcgcccccg gcttcgcatc ctttgttctc taccttccgc gcgaacgcct
     3241 caccgttgtt gccttcagca acatttactc ctcggccaca accaccatcg gatacgatat
     3301 cgccgcgatc gctctcgccc tcccgtatga cacgttcacg gcagcctcgc cccttacaag
     3361 cgccgccatc gccggttcga caggaacctt ccagtttggg cccgatttct accagaggaa
     3421 tgcgaagctc gaattgatct ccgcctcggg cggcctggcg cttcactggc cttccggcga
     3481 tctctcccca ctcatcccgc aatcatcaga tcacttcatt gatcgcagct attgggagaa
     3541 cgtctccatc gagcgcgatg cagcgggtca cgcagtcgct ctcaactacg accgttttcg
     3601 cgggccggtc gtacccacgc cctgaacccg ttgcgccgcg cgaacccgtc gcagcagact
     3661 actccgcccg caatgcctcc actggattca cccgcgccgc ccgtcgtgcg gggatgaaac
     3721 tcgccagcag cgcaaacgca cccagcacca ccgccacccc acccaacgtc gtgagatccc
     3781 acgcccgcac gccaaacagt aggctcttca ttgccgccga ggcgccaatc gacagcccaa
     3841 gccccaccgc gatgccgatg gctgccagca aacccgcctc tttgaggatc agccgatgca
     3901 cggcgccgcg ctgcgcgccc agcgccatcc gcacgccgat ctcgcgcgta cgctggctca
     3961 ccgaatacgc aacgactccg taaagcccta cgacacccag taccagcgcc agcaccgcga
     4021 acccgcccac cagatacgcc gacagctgat gcatcacggc cgtaggcgat tgctcaatct
     4081 gctgctccat cgtgatctct ccgtacacgc ccagggccgg atggatcgcg cgcagtgtct
     4141 tcacaagttc cggcagaaac gccttatcgt ctcctcgcac acgaactgca agagcgaaga
     4201 aagcgtccgg gccactactg atcgaaaaat actctgtggg cagcggatcg ttgtccaggg
     4261 cggcctcgcg cacgtcggcc acgacgccaa ccacctggcg catcgacttc ggtgacagct
     4321 tgatgtcgcc aatcatcttc ccaaccgcat cctcacccgg aaaatatttc cgcgccaggg
     4381 tctgattgat gaccgtcgtc cttggatgat tctcgtcgtc ctgtgcagtg aacaggcggc
     4441 cctccaccag tttcgccccc agcgtccgca tgtaatccga gctgatatcc cgttccataa
     4501 catcgttatg ttcgccgtga aatggttggc caggaatgcg gatccagtcc gtgtcgcaat
     4561 agcactgcac cggcaaatcg ctcgacagcc ccagcgattc gacgccgggc aacccaccca
     4621 ctcgcgtgtc aatctcccgg tacagtgccc tcagttcttc tggcttctgg ctcgcaggat
     4681 cggtcatcat caattgcacg gtcgccagat gcgttggatc aaacccaaga tcgacgtgca
     4741 atagccgata aagactcttg cccagtagcc ccgctccagc cagcagcacc atcgcaatag
     4801 tcagttccac gacaacaagg ttcgcgccca gcttgcgcca gaatcgccct gccgcagtcc
     4861 ggccgccctc gctgagggct tcgtgaatat cacgaacagc gagtcgcatc gccggcgtca
     4921 gcgccatgag caacagagcc agcagagcaa cggttgccgc gaatgcccag gtatgcagat
     4981 tgagtccgac attctgcaga aatggcgccc cctgagcaat ttgtttcgga aacaaactgc
     5041 gcagcagcga catcatgcct gcagccgcta agactcccgc accacagccg gcgatcgcca
     5101 gcaacagcgc ctcggtgatg aattgccgca gcaaccgcgc cggtgtcgcg cccagcgcgc
     5161 cccgcactgc aatctcccgc ctccggcttt ccgatcgcac cagcagcagg ctcgccacgt
     5221 tcacgcacgc gatcagcaac agcaggcccg cgcccgccag caaggtaagc agaatcggcc
     5281 gaaccttgcc gacgatgaac tccgagagcg gctgcacgct ggcaccctgg cccgtattcg
     5341 acgttggaaa ctgaatggca agctgcgctg caatcttttg caggtccgcg cgcgcctgct
     5401 cgattgtcac accgtcacgc agccgcccca cgccgtccag attgtgacag ctccggcgct
     5461 gctcgcatcc gttcttgtcg agtaacggca cccagagttc cgctccgccg cgaggagcga
     5521 acgcgaagtc gcgcggcagc accccgataa tcgtgaacgg atcgccgctc aggctgatct
     5581 tttccccaat cacgtcccgc cgcccgccga agcgcgtcac ccacgtgccg tagctcagga
     5641 tcgcgatctt cgctcctccc ggcttatcct cgcccggtcg aaaatcgcgc cccagaatcg
     5701 gctgcacacc cagggtgtgg aagaacccgt cgctgactcg cgccgccggc accggctccg
     5761 cactcgatcc ccttcgcagc agaaagccgc tgccggtata aacatcgatc gactgcagcg
     5821 tcgtgttcag tcgcttccag tcctcgtagt cgtcgcgtga gatattggag cgcggaaatg
     5881 ccgccgcgct ctcatccaca tccaccagac gattctgctg cgcatacggc agcggctgaa
     5941 tcagcgccgc gtcgacaaag ccgaaaatgg ccgaactcgc gccaataccc agcgccagca
     6001 tcagaatcgc tgtcacagcg aaccccgggc ttttgcgcag ttgccgcagc gcgaatcgca
     6061 gatcctgcgc aatcgtctcc gcccggaatc cgaccgcctc atggctctgc tcgcgaatcc
     6121 gcgtcgcatt gccgaattgc cgcgccgctg cgaaacgcgc cgcctccggc gtcattccgc
     6181 tctcctgcag atccttcgcc atctgctcgc gatggaacgc catctcttcc tcaagttccc
     6241 cggcgtatgt ctcccggccg aagagcagct tcaatttccg ccagaaattt ccgaagc
//
LOCUS       DASZYN010000198         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_327540, whole genome shotgun sequence.
ACCESSION   DASZYN010000198 DASZYN010000000
VERSION     DASZYN010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327540"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..822)
                     /locus_tag="VHE33_05045"
     CDS             complement(34..822)
                     /locus_tag="VHE33_05045"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HVT96852.1"
                     /translation="MRAKLSAGCGVAVVFLLGACAGARAAEPSAGRTPILMELFTSEG
                     CSSCPPIDAWAARLDQFQPIARGQIILLSEHVDYWDHDGWKDPFSSPALTERQSNYER
                     GLGQSNVYTPQVVVDGDVEVHPWKTEEVKSSLDKAALAQTMAVKIDDVALANGELTGK
                     VDADGTGGKHGDVFVAVALEKTETDVLGGENHGKKLTNVGVVQSLVKIGKVEKGKAFV
                     QDFRVKVGQGADAGNLRVVAFVQEPGPGKVLGAAVTKEIGKGGN"
     gene            complement(924..1958)
                     /locus_tag="VHE33_05050"
     CDS             complement(924..1958)
                     /locus_tag="VHE33_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVT96853.1"
                     /translation="MIRPLLALACALSATGLLAQTTEAPAPAPGANTTAPAAGAAGPG
                     ASAPAQQTAPNGQNPQQSGDQGQFTITVPVNEVYLEFAVTDKHGHFIKNLQQNQFALL
                     DDHKAPAQVYSFTQQTNLPLRVGILIDTSTSIRQRFQFEQQAATSFLLEILRPQSDKA
                     FIEGFDVTPDYKQDWTNNLDALTEGIRDMRSGGGTALYDAVYSACRDKLLTSRGQEPV
                     RKAIVLVSDGDDNQSHAYLEDAIKECQRAETIIYAISTDNSPTRDRGDQVLEKMAQAT
                     GGKAFFPPRMEDMANSFALIQEELRSQYALVYKPADFQANGEFRPIYLYCLDRRFMVR
                     VREGYFAPKG"
     gene            2061..>2335
                     /locus_tag="VHE33_05055"
     CDS             2061..>2335
                     /locus_tag="VHE33_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HVT96854.1"
                     /translation="MLATSARPWHNRAMPDSQKSAVSTPHAPAAIGPYSQAIRLGNLL
                     YTSGQIALDPATGQLVPGAIAEQTTRVLENLKAILTAAGTDLARVIKT"
BASE COUNT          395 a          878 c          605 g          457 t
ORIGIN      
        1 aagactatac catgtcatta ccttgattgt accctaattg ccgcccttcc cgatctcctt
       61 cgtaaccgcc gcccccagca ccttccccgg ccccggctcc tgcacaaacg ccaccacccg
      121 cagattcccc gcatccgccc cctgccccac cttcaccctg aagtcctgaa cgaacgcctt
      181 ccccttctcc accttcccga tcttcaccag cgactgcacc accccgacat tcgtcagctt
      241 cttgccgtgg ttctcgccgc ccagcacgtc cgtctccgtc ttttccagcg ccacagcaac
      301 aaacacatcg ccgtgtttgc ccccggtccc atcggcgtcc accttgccgg tcagctcgcc
      361 attggccagc gccacatcgt caatcttcac cgccatggtc tgcgcgagcg ccgccttgtc
      421 gaggctcgac ttcacctcct cggtcttcca cggatgaacc tcaacatcgc catccacgac
      481 tacctgcggc gtgtacacat tgctctgccc caggccgcgc tcgtaattgc tttgccgctc
      541 ggtcagcgcc ggcgacgaga acggatcctt ccacccatcg tgatcccagt agtccacatg
      601 ttcgctcagc aggatgatct gcccgcgcgc aatcggctga aactggtcca gccgcgctgc
      661 ccaggcatcg atcggcggac aactcgagca tccctccgac gtgaacagct ccatcagaat
      721 gggcgtccgc cccgcactcg gttccgccgc ccgcgcccca gcgcacgcgc ccagcagaaa
      781 caccactgcg accccgcaac ccgcggataa tttcgctctc atgctgtgag attatgccga
      841 ctgagtcggt cccgcaccat agccactcgc agcggcggcg gcgctcttcc ggaacgccga
      901 gcgcggaact ccgaacgcca gccctacccc ttgggcgcga agtacccctc gcgcacgcgc
      961 accatgaatc gccggtccag acaatacaaa tagataggtc ggaattcccc attggcctga
     1021 aagtccgcag gcttgtacac gagcgcgtac tggctgcgca gctcttcctg gatcagcgcg
     1081 aagctgttcg ccatgtcttc catgcgcggc gggaaaaagg ctttgccgcc cgtcgcctgg
     1141 gccatctttt ccagcacttg gtcgccgcga tcgcgggtcg gactattgtc cgtgctgatc
     1201 gcatagatga tcgtctcggc gcgctggcac tctttaatcg cgtcttccag atacgcgtgg
     1261 ctctggttgt cgtcgccgtc gctcaccagc acaatggcct tgcgcaccgg ctcctgcccc
     1321 cgcgaggtca gcagcttatc ccggcatgcg ctgtacaccg cgtcatagag cgccgtgccg
     1381 ccgcccgatc gcatgtcccg gatcccttcc gtcagcgcat cgaggttatt ggtccagtcc
     1441 tgcttgtagt ccggcgtcac gtcaaaacct tcaatgaacg ccttgtccga ctgcggccgc
     1501 agaatctcca gcaaaaacga tgtcgccgcc tgctgctcaa actggaatcg ctgccgaatc
     1561 gaagtactcg tgtcgatcag gattcccacc cgcagaggca ggttcgtctg ctgcgtaaag
     1621 ctgtacacct gcgccggcgc cttgtggtcg tccagcagcg caaactggtt ctgctgcagg
     1681 ttcttgatga agtgcccgtg cttatcggtg accgcgaact caagatagac ctcgttcacc
     1741 ggcacggtga tcgtgaactg gccctgatcg ccactttgct gtgggttttg cccgttcggc
     1801 gcggtctgct gggctggcgc agacgcgcct ggtccggcag cgcccgcggc gggagccgtc
     1861 gtattcgcac cgggagcagg ggcgggtgct tcagtcgtct gggccagaag tccggtggcg
     1921 ctcagtgcgc acgccagggc caggaggggt cgtatcacgg cggttctcat tgacggtgtg
     1981 actctatttt accttgtggg gtcaacgccg ccctccatcg aaagcgtcac ctcggccttt
     2041 ccctcgggcc accgcgtacc atgcttgcca cctctgctcg accctggcac aatcgagcca
     2101 tgcccgattc gcagaagtcc gccgtctcca ccccgcacgc ccccgccgcc attggaccct
     2161 actcgcaggc catccgcctc ggcaacctcc tctacacctc cggccagatc gccctcgatc
     2221 cggccacggg ccagctcgtc cccggcgcca tcgccgagca gaccacccgc gtgctcgaaa
     2281 acctgaaagc gatcctcacc gccgccggca ccgatctcgc ccgcgtcatc aagac
//
LOCUS       DASZYN010000199        20865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_390230, whole genome shotgun sequence.
ACCESSION   DASZYN010000199 DASZYN010000000
VERSION     DASZYN010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 20865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20865
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390230"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..497)
                     /locus_tag="VHE33_05060"
     CDS             complement(30..497)
                     /locus_tag="VHE33_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96855.1"
                     /translation="MSAAPLSQQSFDSLDAMMEAYAAEAIRLAEADHGMTLDYTPESI
                     SRLETVIAARGPVPEPEQEEATRLWGAWYGELFRRKYGGDWIMAVYPGQLNSGRSDAG
                     EEVAMPALDIRGSQVYPLLKIFRRLSIGPSEDLAAFFARVSAALEAKPGQTSA"
     gene            626..1336
                     /locus_tag="VHE33_05065"
     CDS             626..1336
                     /locus_tag="VHE33_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96856.1"
                     /translation="MRKWSVRSGVVAMAAAFCMMGMLAEGQVVSPPPNPQLPAPAPLA
                     NVKYNYPWEIYGGFAYSHFDAGPQTFQGANLGGFDARAIRELGTRWGVGVNVRGYYGT
                     SGAEPNCGSCTGSGPNAGPIEGPFVSEHMFLAGPEYRLKSNEHAAMMLHAFVGGAYGN
                     FTSGLNGVPPQNVGFFSNQFAFGGAFGGSIDLNRSPRLVFRIAPDATLTNFGSAGLKE
                     QFAISVGVVYRMGHPRAR"
     gene            1396..2412
                     /locus_tag="VHE33_05070"
     CDS             1396..2412
                     /locus_tag="VHE33_05070"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT96857.1"
                     /translation="MLIKPFIRLMRDGRLSALLGAGAKLKPFYKLTYLAAAGEAGLLN
                     RLATGPATLDSLAEFLVADSGQGKAALEAWLQMGVRLRLLRLEPSGYTLRGLAKLLAR
                     PENDAALAMVEEVAGLHHKLIAATLPRLRKGELWSLDDQDGELIARSSRVLEAFQAEV
                     IRRLFPAQGSLRLLEVGCGSGIYLRHAADRNPGLTAVGVELQPDVAEAARANLRGWGL
                     DGRVTVDDGDIRTRTPGELFDIATLYNNIYYFPVEERVSLLKQVGSFLKPGGFLLLTT
                     LCQGGSLGAEALNLWGAATRGCGRLPSEEELVSQLRQAGYGRVKTMNLIPGERFVAFQ
                     GFWK"
     gene            complement(2486..3532)
                     /locus_tag="VHE33_05075"
     CDS             complement(2486..3532)
                     /locus_tag="VHE33_05075"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HVT96858.1"
                     /translation="MTQSHGYAAQNATTPLAPFKFTRRELHEHDVHVEIQWCGVCHSD
                     IHSVRNEWKNAVYPMVPGHEIVGTVKAVGSAVKKFKAGDLVGVGVIVDSCRTCPSCKA
                     GEEQYCEVGITLTYGQKDKYGDLTQGGYSNNIVTDEHFVHTVSPKLNPAGVAPLLCAG
                     ITVYSPLRHWKIGPNKKVGVVGLGGLGHMALKFAHAFGAHVVQFTTSESKIADAKRLG
                     ADEVVISKDAAAMKKQASSFDFIIDTVSAPHDINPYLEALKRDATYAQVGLPDTPPVI
                     NPGNLIFKRRNWSGSIIGGMAETQEMLDYCAEKGITADVEVIKIQQINEAFERVVKAD
                     VKYRFAIDMSSLGA"
     gene            complement(3646..5490)
                     /locus_tag="VHE33_05080"
     CDS             complement(3646..5490)
                     /locus_tag="VHE33_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HVT96859.1"
                     /translation="MLPKSFPLRLTLLCAALTLLALITPRPARAQQPSFRVADGKFLL
                     NGKPFQVISGEMHYARIPRAYWRDRLRMAKAMGLNAITTYVFWNAHEPRPGVFDFSGN
                     LDIAEFIREAQQEGLYVILRPGPYSCAEWEFGGFPAWLLKDPNMIVRSTNPQFLAAAR
                     TWLLRLGKEVAPLQIGNGGPIILVQVENEYGSYGDDHAYMEDIHHDLVDAGFTQAQLY
                     TADGPEQVPRGSLPELPVGINFGPGESQKGFATLRKLRPSGPFFNSEYWDGWFDHWGA
                     PHAHTNTQQQASDLDWTLRQGYSISIYMFHGGTSFGWMNGANSNGKNYEPDVTSYDYD
                     AALDEAGHPTPKYYAFRDVIARVTGAKLPPVPAPPVVTTIPAFTLERAASLWDNLPKP
                     VHSDQPLAMEQMDQAYGYILYRTTVSGPVNGDLAIGALHDYAQVYIDGKLVGTMDRRL
                     NQTSLPVRIPGAHARLDLLVENTGRVNFGPALPGERVGLLNGVSLAGKSVTGWDNYSL
                     PMLHPETLPFAAKPCTGACYYTATFNVAHLADTFLSTGALGKGEVWINGQPLGRFWHI
                     GPQKALYLPAPWIKVGTNQVVVLDLEGQPNRQLQGLDHPVLSAPIENP"
     gene            5846..6784
                     /gene="glsA"
                     /locus_tag="VHE33_05085"
     CDS             5846..6784
                     /gene="glsA"
                     /locus_tag="VHE33_05085"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HVT96860.1"
                     /translation="MLDDVYHKYKDVDDGSVATYIPELSRANPRHFGICLATVEGRVF
                     TSGDWEQEFTIQSMCKPFAFQMALEKHGPQKTLLHIGLEPSGEAFNSIELDPKTGRPF
                     NPMVNAGAIAVASLIRQNPVQAGIAAFVEKLSRAAGRHLRIDYSVLASETETGNRNRA
                     IGYLLLNAGIIDEQVQQSLDQYFAQCSVLVNCRDLALMAATIANMGNHPIDAQPVFDF
                     QFLKYVMAVMFSCGLYDYAGNWAVEVGLPAKSGVSGGIFGVVNRQLGIAVYSPRLDAQ
                     GNSVRGILACRELAGHFGLHTFDFSNVGSSFMQWLL"
     gene            complement(6828..7529)
                     /locus_tag="VHE33_05090"
     CDS             complement(6828..7529)
                     /locus_tag="VHE33_05090"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HVT96861.1"
                     /translation="MDPNKLIIAIFEFAVLLFSLSLHEAAHAWMASRLGDPTARMLGR
                     ITLNPVKHIDLWGTIILPAIMLLINPGFLFGWAKPTPVTTSNFKNLTRDDILTTVAGP
                     GSNVLAAIAATIVLLILSHISPAGSALVHQMAGSGGFDPALMQSSPVLVPLVILFFLG
                     VELNLILAAFNLLPLPPLDGSHVFRHFLPYKALRIYDSLGIISLFVIFIWGGPVIGFL
                     IHPALSLFSRILLAF"
     gene            complement(7641..7970)
                     /locus_tag="VHE33_05095"
     CDS             complement(7641..7970)
                     /locus_tag="VHE33_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96862.1"
                     /translation="MTVEEVEAVIHLQVNEHPTATNAHQLVLKDALVRPRRISVIEKT
                     VKDGQVFDQELRVWLIADERPGDGYKIVMSDDGLRFWLASSGFSTDGHLVVSGWYNSL
                     LAAFLGM"
     gene            complement(8029..8877)
                     /locus_tag="VHE33_05100"
     CDS             complement(8029..8877)
                     /locus_tag="VHE33_05100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HVT96863.1"
                     /translation="MPRYQYREIEPTREAKEVFNRWVDYLDDEFSRHTSVVLRAEIVR
                     DNLHQLLLGRPHGGRLNFTLATELPFNVLQLSLDPANVTLEPEYYGDIDAERYALIKP
                     LIWFWRMFDRSPVALNHWLGFRLRSMLGRHIFKSIGSNVKIYHGVEFTFGYNLTLEDD
                     VIIHNHVMLDDRGELVIRKGTSISEHAAVFSHAHDPVEACIIEHRRTEVGPGARVTYR
                     SLVMPGVRVGEDSMLGAQAVATHDLEPHSIVGGIPGRRIMVKNEATRLGEKDPTDPCA
                     ELPPHD"
     gene            9006..9830
                     /locus_tag="VHE33_05105"
     CDS             9006..9830
                     /locus_tag="VHE33_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96864.1"
                     /translation="MDETPEVPVQRSYSGLFMGALAVAVICALAGLIWSYTLASRLTS
                     AEAQVKSAQQQNQKLESALEQTNARLKVTTETLGQSLGMTQKQLEQKAEDLLARQQAS
                     AKQLQAAQRQISSVSNDVSGVKTDVGGVKTDLGNTKTQLSNDEAAMTAMKGDMGLQSG
                     LIATNARELDILKHKGDRNYYEFTLGKNQKQAVATVALELKKADPKHSKFTLVVYSDD
                     KAIQKKDRNLDEPIQFYTGKDHLLYELVVNDIAKNQIKGYIATPKNAPAPVTTSGE"
     gene            10141..10833
                     /locus_tag="VHE33_05110"
     CDS             10141..10833
                     /locus_tag="VHE33_05110"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HVT96865.1"
                     /translation="MNKSSNGGFSFGQRVALWGVPRLTAALLALVGSTLRFEVIAEEG
                     AVPPEPPARGIFCFWHRCTLPAGWYFRKFRCSILISQSFDGELIARTLGLLGYSSVRG
                     SSSRGGAAGLMALRDVLERGEPVVFTADGPRGPIYQTKIGPVKLAAMSGEEIGCFYLL
                     PARAWVARSWDKFLVPLPFTRVVVSWARAVRAPAADADAATLEAKRMELNEAMERARL
                     RAEKHLGHSSDK"
     gene            10846..11901
                     /gene="queA"
                     /locus_tag="VHE33_05115"
     CDS             10846..11901
                     /gene="queA"
                     /locus_tag="VHE33_05115"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HVT96866.1"
                     /translation="MLVSDFDFDLPENLIAQEPAADRAGSRMLALDRTTGSWQDRCFR
                     ELPALLRPGDLLVLNDSRVIPARLFAHRAGLHTQASHRVTGRVQVLLAEQISEWEWKA
                     LVRPGRKVLTGEHLDFGNGALRAEVLAHGEFGERTLKFEPVADFFGTLERLGHMPLPP
                     YIHREDRAGDRERYQTVYARERGSVAAPTAGLHFTPEILDEIRARGIETATVTLHVGL
                     GTFQPVRVDRVEDVKLHRERYAISEETAAALNAARREGRRVVAAGTTTVRTLEHCALQ
                     GGDFTAHAGSTSIFISPGFEFRVVSALLTNFHLPQSTLLMLVSAFAGREKVLAAYRHA
                     VEAGYRFFSYGDCMLIA"
     gene            11959..13011
                     /locus_tag="VHE33_05120"
     CDS             11959..13011
                     /locus_tag="VHE33_05120"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HVT96867.1"
                     /translation="MMPAPSRTVLLGSLLRSVSRSFYLTLRVLPAGMREPVGLAYLLA
                     RAADTIVDTSLLLPELRREFLLGFRASVNGSGDEALLDRIAVEAGAQQRDSDEKTLLE
                     SLRPALALLSQLNTADRDAVREVVTTLTQGMEFDLRIFPPESSGQLAALDEYSDLDSY
                     TYFVAGCVGEFWTRMTWAHAPGSLAGEPEAMLERGVRFGKALQLTNILRDCGKDLRIG
                     RCYLPSSILARYGLTPNDLLRPENSQRTRPLLFELVRRDLDHYRAAMDYTLALRPSSR
                     RLRLACLWPVLIGLETMALLVRNEAWLDPAKASKIRRSDVYKIVARSLVLASSNRRLK
                     LWMDSLIAKVEEGMQS"
     gene            complement(13032..15092)
                     /locus_tag="VHE33_05125"
     CDS             complement(13032..15092)
                     /locus_tag="VHE33_05125"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HVT96868.1"
                     /translation="MTYRLLPLALAAALFPACFAQSTSAPPTEHPLDAIPYSPSFDLA
                     DMDRAADPCVDFYQYVCGGWMKNNPIPPDQAAWDVYAKLTDDNRKLLWGILEKDAKLT
                     HRTPAQQKIGDYYAACMDTNAIDQRGLAPLQPELDRIAGIQDRKQIAAFLRDGADQRG
                     LYFLGAGSEPDPKNAGSMIVGIEAGGLGLPDRDYYTKTDAHSQDIRQHFVAYMAHLLV
                     LAGEPQTDADRDAAATMKIETALANASLTRVERRDPYNTYHPMKVEAIDSLVPALELG
                     AYLKKQKVPEAVLTNVDVAQPKFLAALQTELATEPLGSLRAYLRIHALNLAASSLSSN
                     FEEARFDFYSKYLRGVPQMPARWKRCEGQVDRSMGEALGQEFVARTFSPDTKQKVLVM
                     TQQIEQAMQEEIQNLDWMSPATKQAAIVKLHQVRNKIGYPDKWRDYSSLTIDPHDYFG
                     NVLRARAFEEKRDWAKVGKPVDKSEWFMSPPTVNAGYDPQINDITFPAGVLQPPLYDA
                     KEDDAPNYGDTGGTIGHELTHGFDDEGRQFDGQGNLRDWWTAADAKGFEDRINCQREQ
                     YATYVIVDDIHINSKLTSGEDVADLGGTLLAYLAWKKQTAGMHLENKEGFTPDQRFFV
                     GYAQWACENERPENLRVSAITNPHSPGKYRVNGIVSNLPQFAQAFGCKAGQPMVRAKV
                     CKVW"
     gene            15197..17956
                     /gene="polA"
                     /locus_tag="VHE33_05130"
     CDS             15197..17956
                     /gene="polA"
                     /locus_tag="VHE33_05130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HVT96869.1"
                     /translation="MANETSKPTVFLLDAMSFIFRAYHAMQRSRPMSTRTGVPTAATY
                     VFINMLNKLRKDFSPEYFAAVFDVSGPVFRDERARGMTVKKYSAETQQFETVETGGYK
                     AKREAMPPDLAQQLPYVRRALEAFRIPILEAQGFEADDVIGTLATKAATGGHPVLVVS
                     NDKDMMQLVNEHVKILNPVKDNLLLDREKVIETLGVPPEKVVDVMALRGDSIDNIPGA
                     PGIGDKGSVELIQQFGSVEAALDRAEEIKRKTYRESLQNNREAVLLSKELVTIHCEVP
                     LDFDLGAMRTQEPDVAACRALFTELEFTTLLRELAPAVPAHAVEYVLEPTAEQLAAFM
                     KQAQKDGFALALPASELQAAAEQVSEQEGEALEEKEPELKTMSLLDLVDQAEAKCETA
                     QAVAECRLGVAAGSDGALLVPLDAVRTLLEDAKVPKHVHDLKAALRVCEAHGVALQGA
                     TDDLMLYSYLINPTHATHRLGDVAARFGGGPPHETGDRALAEAAFLTEALTKMMRDEV
                     AALEERRVYDEIDLPLVPVLLGMEKEGVRIDSAELSKMSAGLSKQMDRLSEEIFALAG
                     QRFNINSPKQLGDVLFNKMGLPRPLKYGKGKVISTAQDVLEELAVHNDVPRLVLEFRQ
                     LAKLKSNYSDSLPLLADSEGRVHTTFNQVGTATGRLSSTNPNLQNIPIKTELGREIRA
                     AFIAAPGCVLLSADYSQIELRLLAHFSADPLLVKAYQTEQDIHTLTAAEVFGVPAETM
                     SKETRNRAKAVNFGIVYGISPFGLAAQLGIDQHEARLYIETYFERYAGVRVYIDRMLE
                     ETRREQKVRTMFGRVRPIPDIQSRNANQRGFAERTAINTPLQGTAADLIKLAMIRIDK
                     RLAKEKLKARMTLQVHDELVFDVPEDEIEPMKELVRHEMEHVVELSVPLVADVGVGKN
                     WRDLE"
     gene            complement(18380..19723)
                     /gene="glmM"
                     /locus_tag="VHE33_05135"
     CDS             complement(18380..19723)
                     /gene="glmM"
                     /locus_tag="VHE33_05135"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HVT96870.1"
                     /translation="MRKLFGTDGIRAVAGEAPLDAKTIYAVGLALAHQLNGSHAQPRV
                     LLGMDTRESSSWIAAIIAAGLTEGGAAVENAGVITTPGIAYLARQHGFSAGIVISASH
                     NPWQDNGIKVFGGDGYKLPDETELRIEAEIFRRLEQAQVPASGSAPAVNDRYRKDYEQ
                     FLRSAFPDLNLSGLRVVLDCANGAASVIAPELFDHLGGTIHLTHASPDGRNINANCGA
                     LHPEVVAAETKNLNADLGVTFDGDADRAMFADAHGNVVNGDAIMLLAARDMKERGLLR
                     NDTVVATTMSNMGLEAALRRDAIRMLRAPVGDKYVLERMQKEQASLGGEQSGHILFPH
                     LATTGDGLLTALVVLDIIRRSGRALHELIADLKVFPQVIVNVRVREKKPLDQIATVAD
                     TIRAAEVELAESGRVVVRYSGTEALARVMIEAESEEAMRRHADRIADAIRAELGA"
     gene            complement(19720..>20865)
                     /gene="xseA"
                     /locus_tag="VHE33_05140"
     CDS             complement(19720..>20865)
                     /gene="xseA"
                     /locus_tag="VHE33_05140"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF014640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HVT96871.1"
                     /translation="FKPADGLHVLLRGKISVYEQRGQMQLVAEFLEPVGAGSLQIAFE
                     QLRQRLETRGFFDPAAKQPLPAFPHCVGIVTSPTGAVIRDFINIVNRRHAGLHVLLYP
                     ALVQGEAAAAEVAAGIAHFNRSRSVDVIVIARGGGSLEDLAPFNSEFLAEAIFNSQIP
                     VISAVGHETDFTIADFVADLRAPTPSAAAEIVTAALHNVAEQVQALDQRLVRAARYRL
                     MQSSQAMARVRVEAILIRERDHLRRRQQRVDDLALRLESQWYLRHRRLSDKLHHLAAR
                     LQRQDVIARTGIARERLTALEARMIRAQRARFRTSRDRESAIARQLQSLSPLAVLSRG
                     YALVYDERGMLIKDTENITQGQSIVARLARGRIRSRVTAVEKETQGS"
BASE COUNT         4049 a         5631 c         7184 g         4001 t
ORIGIN      
        1 agcgagggga cagcggttag cggatagctc tacgcagacg tttgccctgg tttggcctca
       61 agagccgccg aaaccctcgc aaagaaggcc gcgagatcct cagaaggccc gatgctcaga
      121 cggcggaaga tcttcaacaa cgggtagacc tgggagccgc ggatatcgag ggcgggcata
      181 gccacctctt cgccggcgtc ggagcgtccg gagttgagct ggccgggata aacagccatg
      241 atccagtccc cgccatactt tctgcgaaag agctctccgt accaggcgcc ccacaggcgg
      301 gtggcttcct cctgttcagg ctcgggcacg gggccgcgcg cggcgatgac ggtttcgagg
      361 cgagaaatcg actcgggcgt gtagtcgagc gtcatgccgt ggtcggcttc ggcaagacgg
      421 atggcttcgg cagcgtacgc ttccatcatg gcgtcgaggg agtcgaacga ttgttgggag
      481 agaggggcag ccgacatccc tatcagagta caggaccagc gcagcctggg ccgagatcgg
      541 gacagaaggc ggttcgccca tgtatctttg aagcttgcga cacattgcag ggcttgcccc
      601 gaagtgctgg aggaatggcc ggttgatgag gaagtggagt gtgcgttctg gcgtggtggc
      661 gatggctgcc gcgttctgca tgatgggaat gttggccgag ggacaggtgg tgagtccgcc
      721 gccgaatccg caactgccgg ctccggcccc gctggccaac gtgaagtaca actacccgtg
      781 ggaaatctat ggcggcttcg cctattcgca ttttgatgcg ggaccgcaga cttttcaggg
      841 cgcgaacctg ggcgggtttg acgcgcgggc aatccgcgaa ctgggaaccc gctggggcgt
      901 gggcgtgaac gtgcgcggct actacggcac cagcggtgcc gagccgaact gcggcagctg
      961 caccggcagc gggccgaatg caggtcccat tgaaggaccc ttcgtaagcg aacatatgtt
     1021 cctggccggg ccggagtatc gcctgaagtc gaacgagcat gccgccatga tgctgcacgc
     1081 ctttgtcggc ggggcctatg gcaacttcac cagcgggctc aacggcgtgc cgccgcagaa
     1141 cgttggcttc ttctctaacc agttcgcgtt tgggggagcc ttcgggggat cgattgacct
     1201 gaaccgatcg ccaagactgg tgttccggat tgcgccggac gcgacgctga cgaacttcgg
     1261 cagcgccggt ctcaaggagc agttcgcgat ctcggtcggt gtcgtgtaca ggatgggtca
     1321 tcccagggct cgatagcgcc cccccgacac ccacccggca tcgttcgtct ccctgttcga
     1381 ccataatgaa ggacgatgct gatcaaaccc ttcattcgtc tcatgcgcga tggccggctc
     1441 agcgctttgc tgggtgccgg cgcaaagctg aagcccttct ataaactcac gtatctcgcc
     1501 gcagccggag aggcgggtct gctgaaccga ctggctaccg ggccagcgac gctggactcg
     1561 ctcgctgaat tccttgttgc tgacagcggc caggggaaag cggccctgga ggcatggctg
     1621 cagatgggcg ttcgcctgcg actgctgcgc ctggagccca gcggatacac gctgcgggga
     1681 ctggcgaagc tgctcgcgcg gccggagaac gatgccgcgc tcgccatggt cgaggaggtg
     1741 gcgggtctcc atcacaagct gattgcggcg acgctgccca ggctacgcaa gggcgaactg
     1801 tggagtcttg acgaccagga cggagaactg attgcccgtt cctctcgcgt gctggaggct
     1861 tttcaggcgg aggtcattcg gcgccttttt ccagcgcagg ggtcgctgcg tctgctggag
     1921 gttgggtgcg gctccgggat ctacctgcgt cacgcggccg acagaaaccc ggggctgacg
     1981 gcggttgggg tggagttaca gccggatgtg gcggaggccg cgcgggcgaa tttgcgcggc
     2041 tggggactcg atggccgtgt cactgtggac gatggcgaca tccgcacgag aacaccgggc
     2101 gaactgttcg acatcgcgac gctctacaac aacatctact acttcccggt cgaggagcgc
     2161 gtgtcgctgc tcaaacaggt gggaagcttt ctgaaaccgg ggggattcct gctgctgacg
     2221 acgctgtgcc aggggggcag tctgggggcg gaggcgctaa acctgtgggg agcggcgacg
     2281 cgaggctgcg ggcggctgcc gagcgaagag gaattggtga gccagttgcg ccaggccggt
     2341 tatggccggg tcaagacgat gaacctgatt cctggagagc gcttcgtagc gttccagggt
     2401 ttttggaagt agtgcaaacg gtggcggcca catctgacca acccggcaga tatggccacc
     2461 acgctgagcg cgcctccggt tctgcttagg cgcccaagga cgacatgtcg atcgcgaagc
     2521 ggtatttcac gtcggctttg accacgcgct cgaaggcctc gttgatctgc tggattttga
     2581 tcacctcgac gtcggcggta attcccttct ccgcacagta gtcgagcatc tcctgggtct
     2641 ctgccatgcc gccaatgatc gatcccgacc aattacggcg tttgaaaatg aggttgccgg
     2701 ggttgatgac gggcggcgtg tccggcaggc ccacctgagc ataggtcgcg tcccgcttga
     2761 gggcttcgag atacgggttg atgtcgtgcg gagcggagac ggtgtcaatg atgaagtcga
     2821 aggagctggc ctgcttcttc atcgcggcgg cgtctttcga aatgacgact tcgtcggcgc
     2881 cgagacgttt ggcgtcggcg atcttcgatt ccgacgtggt gaactgcacg acgtgcgcac
     2941 cgaaagcgtg ggcgaacttg agcgccatgt ggccgaggcc cccgagcccg acgacaccga
     3001 ccttcttgtt ggggccgatc ttccagtggc gcagggggga gtagacagtg ataccggcac
     3061 agagaagcgg cgccactccg gcagggttca gcttcggcga gacggtgtgg acgaagtgct
     3121 cgtcggtcac aatgttgttg gagtatccgc cctgggtgag gtcgccgtac ttgtccttct
     3181 ggccgtaggt aagcgtgatg ccaacctcgc aatactgctc ttcgccggct ttgcagctgg
     3241 ggcatgtgcg gcaggagtcg acgatgacgc cgacgcccac caggtcaccg gccttgaatt
     3301 tcttcacagc ggatcccacg gctttgacgg tgccgacgat ttcgtgtccg gggaccatgg
     3361 ggtagacggc gtttttccac tcattgcgga cggagtggat gtcggaatgg cagacaccgc
     3421 accactggat ttcaacgtga acgtcgtgct cgtgaagttc gcggcgagtg aatttgaagg
     3481 gggcgagcgg agtggtggcg ttttgcgctg cgtagccgtg ggactgggtc atgaaagagg
     3541 actccttcga tagtgaatgg gaagggtgaa gggggagtca ggtgcagaat ccgtctactg
     3601 gtttagatta tcgcgacgga gtcgagattc ctttgtcgtc ccgcatcacg gattctcgat
     3661 cggagcgctg agtacggggt ggtcgagacc ctggagttgg cgattgggct ggccctcgag
     3721 gtcaaggacg accacctggt tcgtgccgac tttgatccag ggagcaggca ggtagagcgc
     3781 cttctgcgga ccgatgtgcc aaaagcgacc cagaggctgg ccgttgatcc acacttcgcc
     3841 cttgccgaga gcgccggtgg aaagaaaggt gtcggcgagg tgggcaacgt tgaacgtcgc
     3901 ggtgtagtag catgcgccgg tacagggctt cgcagcgaag gggagcgtct cgggatggag
     3961 catgggcagg gagtaattgt cccaccctgt gactgacttg ccggcgaggc tgactccgtt
     4021 gagcaggcca acgcgctcgc caggcagcgc ggggccgaag ttgacgcggc cagtgttctc
     4081 gacgaggagg tcaaggcgcg cgtgggcgcc cgggatgcgg accggcagcg aggtctgatt
     4141 gaggcggcgg tccatggtgc caacgagttt gccgtcgatg tagacctggg cgtaatcgtg
     4201 aagggcgccg atagcgaggt cgccgttgac ggggccggaa acggtggtgc ggtagaggat
     4261 gtagccgtag gcctgatcca tctgttccat ggcgagaggc tggtcggagt ggacgggttt
     4321 ggggaggtta tcccacagcg aggcggcgcg ctcaagggtg aacgcgggga tggtggtgac
     4381 gactggcggc gcgggaacag gcggcagttt ggcgccggtg acgcgggcga tgacgtcacg
     4441 gaaggcatag tacttcggcg tgggatggcc ggcttcgtcg agggcggcgt cgtagtcgta
     4501 gctggtgacg tcgggctcgt agtttttgcc gttggaatta gccccgttca tccatccgaa
     4561 gctggtgccg ccatggaaca tgtaaatgct gatggagtaa ccctggcgga gagtccagtc
     4621 gagatcgctg gcctgctgct gggtattggt atgggcgtgc ggcgcgcccc agtgatcgaa
     4681 ccagccgtcc cagtactcgc tgttgaagaa gggcccgctg gggcggagtt tcctcaatgt
     4741 ggcgaagcct ttctgtgatt cgccgggacc gaagttgatg ccgacgggca gttccggcag
     4801 gctgccacgc gggacttgtt cgggaccatc cgcggtgtag agttgcgcct gggtaaagcc
     4861 ggcatcgacc aggtcatggt ggatgtcttc catgtaggcg tggtcgtcgc cgtaggagcc
     4921 gtactcgttt tcgacctgaa cgaggatgat ggggccgccg ttgccgatct ggagcggggc
     4981 gacctctttg cccaggcgga gcagccaggt gcgcgcggcg gcgaggaact gcgggttagt
     5041 ggagcggacg atcatgttcg gatctttgag gagccaggcg gggaaaccac cgaattccca
     5101 ctccgcgcag gagtaagggc cgggccgcag gatgacgtag aggccttcct gctgggcttc
     5161 gcggatgaac tcggcgatgt cgaggttgcc ggagaaatcg aagacgccgg ggcggggctc
     5221 gtgggcgttc cagaagacgt aggtcgtaat ggcgttgagc cccatggctt tggccatgcg
     5281 gaggcgatcg cgccagtaag cgcgcggaat gcgggcgtag tgcatttctc cggagatgac
     5341 ctggaatggc ttgccgttga ggaggaattt tccatcggcg acgcggaagg agggctgttg
     5401 tgcgcgcgcg gggcggggcg tgatgagggc gaggagagtg agagcggcgc aaaggagagt
     5461 aagccgaagc gggaatgatt tgggaagcat ggttttaccg accgttacgg attatatttc
     5521 gcgagccagc cggcggacgc ctgcatgacg cgggagcagt tccggaggac agaaagagat
     5581 caacgaaccg aattcgcgcc gtcgccgggc ccgcgatcct catagattct tcatatccag
     5641 gctggaataa tcggctccct accttccgat cctccgatga accctgattg agggaccatc
     5701 ccgggaccct tcctgcggga gggcgctgtt tcttcatttt tgtcatgagg agctgaacgg
     5761 aatgcaaggt gcttcagatc gtggaggttc ccaggccggc gcatctccag cgataagtcc
     5821 atggcgagag tcacaaatcc agaaagtgct cgacgatgtt tatcacaagt acaaggacgt
     5881 ggacgatggg agcgtggcaa cttacattcc cgaactgagc agggcgaacc ccaggcactt
     5941 cggtatctgt ctggccacgg tggaggggcg cgtttttacg agcggcgact gggagcagga
     6001 gttcaccatc cagtcgatgt gcaagccgtt cgccttccag atggcgctgg aaaaacatgg
     6061 tccgcaaaag acacttctgc acatcggcct ggagcccagc ggggaagcct tcaattccat
     6121 cgaactggat ccgaagacgg gacggccttt caacccgatg gtgaacgcgg gcgcgattgc
     6181 cgtggcttcg ctgatcaggc agaatccggt acaggccggc attgcagcct tcgtcgaaaa
     6241 actttcccgg gccgccggcc gccacctgcg catcgactat tcggttctcg cgtcagagac
     6301 tgaaacgggc aaccgcaatc gcgccatcgg ctatctgctg ctcaacgccg gcatcatcga
     6361 cgagcaggtt cagcaatcgc tggatcagta cttcgcgcaa tgctcggtgc tggtgaactg
     6421 ccgggatctg gctctgatgg cggcgaccat cgccaatatg ggcaaccatc cgattgacgc
     6481 acagccggtc ttcgattttc agttcctcaa gtacgtcatg gcggtgatgt tcagctgcgg
     6541 actctatgac tatgcgggca actgggccgt ggaagtggga ctgccggcga agagcggggt
     6601 ttccggaggc atcttcggcg tggtgaatcg gcaactcgga attgcggttt attcgccgcg
     6661 gctcgatgcg cagggcaaca gcgtgcgcgg cattctcgcc tgccgcgagc tggcgggcca
     6721 ctttgggctg cacacgttcg acttcagcaa cgtcggctcg agcttcatgc aatggctatt
     6781 gtaaggaagc agttttcgac gggtgttcgt ggatcggagc tcggaagtca aaaagcgaga
     6841 agaatacgac tgaacaggct gagggcggga tggatgagaa agccgatgac ggggccgccc
     6901 cagatgaaga tgacgaagag gctgatgatg ccgagtgagt cgtagatacg gagcgccttg
     6961 tagggcagga agtgacgaaa gacgtgggag ccgtcgaggg gaggcagggg aagcaggttg
     7021 aaggcggcaa ggatcaggtt gagttccaca ccgagaaaaa agaggatgac cagcggcaca
     7081 agcacagggc tggattgcat gagagcagga tcgaagccgc cagagccggc catctgatgc
     7141 acgagggccg agcccgcggg gctgatatgg gacaggatga gcaggacaat cgtggcagcg
     7201 atggcagcaa gcacgttgct acccggaccg gccacggtgg tcaggatgtc atcgcgggtg
     7261 aggtttttga agttggaagt ggtgaccggc gtcggcttag cccagccgaa cagaaacccg
     7321 gggttgatga gcagcatgat ggcgggcagg atgatggtgc cccagaggtc aatatgcttg
     7381 accgggttca gagtgatgcg gccgagcatg cgcgccgtgg gatcgcccag acgcgaagcc
     7441 atccacgcgt gagctgcctc gtgcaggctg agggaaaaga ggagcaccgc gaactcgaag
     7501 atcgcgatga tgagcttgtt cggatccatt tggaagacga gttgatggta acaggaacga
     7561 agctaagaac cggtcaggta aacgcggtca gctaaaaagc ggtcggctga tgaagcggtt
     7621 agctgcgcag gttgaacgag ctacattccc aggaatgccg caagcaagct gttataccag
     7681 ccgctcacga ccagatgtcc atcagttgaa aaaccgctgg aagctagcca gaatcgcagc
     7741 ccgtcgtcgc tcatgacgat cttgtatcca tcgcccggac gttcatcggc tatgagccaa
     7801 acgcgaagtt cctggtcgaa cacttgtcca tctttaacgg tcttctcaat aaccgatatc
     7861 cgccgtggcc gtaccaaagc atcttttaag acgagctgat gagcatttgt cgcggtcgga
     7921 tgctcgttca cctgcaaatg aatcactgct tccacttcct caactgtcat cgcagctcca
     7981 ggcgggttct tctctgaccg gttctcagct gaccgcttct tagccgcctc agtcgtgcgg
     8041 gggaagttcg gcgcatgggt ccgtggggtc cttttcgcca aggcgagtcg cttcattttt
     8101 caccatgatc ctgcgacccg ggatgccacc cacgatcgaa tgcggctcga ggtcgtgcgt
     8161 ggcgaccgcc tgggcgccaa gcatagaatc ctcgccgaca cgcaccccgg gcatgaccag
     8221 ggagcggtaa gtcacgcggg cgccgggtcc gacctcggtg cggcggtgtt cgatgatgca
     8281 cgcttcaacg gggtcgtgcg cgtgcgagaa gacggcggcg tgttcggaga tggaggtacc
     8341 tttgcggatg acgagttcgc cgcggtcatc gagcatgacg tggttgtgga tgatgacgtc
     8401 gtcttccagc gtcaggttgt aaccgaaggt gaactcgacg ccatggtaga tcttcacgtt
     8461 gctgccgatg gatttgaaga tgtgccggcc gagcatggag cgaagccgaa agccgagcca
     8521 gtgattgagg gccaccggcg agcggtcgaa catgcgccag aaccaaataa gcggtttaat
     8581 cagggcatag cgctcggcgt cgatgtcacc gtagtactcg ggttcgagcg tgacgttggc
     8641 cgggtcgagc gagagctgga ggacgttgaa aggcagctcg gtggcgagcg tgaagttcag
     8701 gcggcctccg tgggggcggc cgagcagaag ctggtgaaga ttgtcgcgga cgatctcggc
     8761 gcgcaaaacg acactggtat ggcgggagaa ctcgtcatcg agatagtcca cccagcggtt
     8821 gaagacttct ttggcttcgc gggtgggctc gatctcacgg tactgatagc gcggcatatg
     8881 tcctagatta cccgcctccc atgtgggttg cagaacaatg gacccggttc gattcgtcat
     8941 tgcgggggtt gtccgtcctg ggatacagtt cgcacaagtg gttgccgaaa ggaagagtga
     9001 caactatgga tgaaacgcct gaagtgccgg tacagaggag ctattcgggg ctgtttatgg
     9061 gagcgctggc ggtggccgtg atctgcgctc tggcgggatt gatctggagc tatacgctgg
     9121 ccagccggct gacaagcgcc gaggcgcagg tgaaatcggc gcagcaacag aaccagaagc
     9181 tggagtcggc gctggaacag accaacgctc ggctgaaggt gacgacggag acgctggggc
     9241 aatcgctggg gatgacccag aagcagctgg agcagaaggc ggaggatttg ttggcgcgcc
     9301 agcaagccag cgcgaagcaa ctgcaggcgg cgcagcggca gatcagctcc gtatcgaatg
     9361 acgtttccgg tgtaaagacg gatgttggcg gcgtgaaaac cgatctcggc aatacgaaga
     9421 cacagctttc gaacgatgaa gcggcgatga cggcgatgaa gggcgacatg ggcctgcaga
     9481 gtggactgat cgccacgaat gcaagggagc tggacattct caagcacaaa ggtgaccgca
     9541 attattacga gttcaccctg ggcaagaatc agaagcaggc ggtggcgacg gtggcgctgg
     9601 agctaaagaa ggccgatccg aagcacagca agttcactct ggtggtctac tccgacgata
     9661 aagccattca gaagaaggac cggaatctgg atgagccaat tcagttctac accggaaagg
     9721 accatctgct gtacgagctg gtggtgaacg atatcgcaaa gaaccagatc aagggctaca
     9781 ttgcgacgcc gaagaatgcg cctgcgccag tgacgacttc gggggaatag tgattgagct
     9841 tccgaagaaa tggtaaacag gcgtagtccg cgctaggcca tggcacagag ggcacaggcg
     9901 agaagcaata gcgctatgac gaggcgtttc atcgcgatcg actcggtcga gagattggcc
     9961 cgatagaaga atcatcggcc gatttcgatg cccgtgctgt agcacgatag tgggaaagag
    10021 cacgacattt ggtgcgtcga aaggtgactg ctgtgggagg caccgcttca tgtgggggac
    10081 gcagcgaatc gttcgactgg cgctgccagc cctgcccgac cggtagctac aatcgatgag
    10141 gtgaacaaga gcagcaacgg gggcttcagc ttcggtcagc gcgtggccct ttggggggtt
    10201 ccaaggctga cagccgcgtt gctggctctg gtgggatcga ccctgcgatt tgaagtcatc
    10261 gcggaggaag gcgcagttcc acctgagcca cctgcgcggg gaattttctg cttctggcat
    10321 cgctgcacgc tgcccgccgg gtggtacttc agaaaatttc gctgctcgat ccttatcagc
    10381 cagagctttg atggggagct gattgcccga acgctggggc tgctgggata cagcagcgtc
    10441 cgcggctcaa gttcaagggg cggcgcggcg ggactgatgg cgctgcgcga cgttctggag
    10501 cggggcgagc ccgtggtgtt tacggcggat gggccgcggg ggcctatcta ccagactaag
    10561 atcggcccgg taaagctggc agcaatgagt ggcgaagaga tcggatgctt ctacctgcta
    10621 cccgcgcgtg cctgggtagc acgctcgtgg gataagtttc tggtccctct tcccttcact
    10681 cgtgtggtgg tgagctgggc gcgggcggtg cgggctccgg cggcggatgc tgacgccgca
    10741 acgctggagg caaagcgcat ggaactgaac gaggccatgg agcgggcgcg cctgcgagcg
    10801 gagaagcatc ttggacatag cagcgacaaa tagaatcgga gtgggatgct ggtttctgac
    10861 ttcgatttcg atcttcctga aaatctgatc gcccaggaac ctgcggcgga tcgcgcagga
    10921 tcacggatgc ttgctttgga ccggacgact gggagctggc aggaccgctg tttccgcgag
    10981 ctgcctgctt tgctgcgacc gggcgatctg ctcgtgctca acgacagccg cgtgattccg
    11041 gcgcggctgt ttgcgcaccg agcgggacta cacacccagg caagtcatcg cgtgacgggc
    11101 cgcgtccagg tgctgctggc agaacagatc tccgagtggg agtggaaggc gctggtgcgg
    11161 ccggggcgca aggtgctgac tggcgaacat ctcgatttcg gcaacggtgc actgcgtgcg
    11221 gaggtgctgg cgcacggcga gttcggcgag cgcacgctca agtttgagcc ggtggcggat
    11281 tttttcggca cgctggaacg gctgggacac atgccgctgc cgccgtatat ccatcgcgaa
    11341 gatcgagccg gggaccgtga gcggtaccag acggtctatg cgcgagaacg cggatcggtg
    11401 gcggcgccaa cggcaggact gcacttcacg ccggagattc tggacgagat tcgcgcacga
    11461 ggcatcgaga ctgccaccgt gacgctgcac gtcggcctgg ggacgtttca gccggtgcgc
    11521 gtcgatcggg tcgaggacgt gaagctgcat cgggagcggt acgcgatttc cgaagagacc
    11581 gcagcagcgc taaatgcggc ccggcgcgag ggacgccgcg tggttgcggc gggaacaaca
    11641 acggtgcgaa cgctggagca ctgtgcgctc cagggcggcg atttcacagc gcacgccgga
    11701 tcgacgagca tttttatttc gccgggtttt gaattccgcg tggtcagcgc cctgctgacg
    11761 aattttcatt tgccgcagtc gacgctgctc atgctggtga gcgcgtttgc aggccgggag
    11821 aaggttctgg cggcgtatcg acatgcggtc gaggcggggt atcgattctt ctcttatggc
    11881 gactgcatgc tgattgcctg aacttcaggg cggattcgtt cctgaccgat agaatcagag
    11941 cgacccacga acacccgcat gatgcctgcc ccgtctcgca ccgttttgct cggctccctg
    12001 ctcaggagcg tctcgcgctc gttttacctg acgctgcggg tgttgccggc aggaatgcgc
    12061 gaaccggtgg gcctggccta tctgctggcg cgcgctgctg acacgatcgt ggacacctcc
    12121 ctgcttctac ccgaactgag gcgggaattc ctgcttggct ttcgtgcgtc cgtgaacggg
    12181 tccggcgacg aggcgctgct ggaccgcatc gcggtcgagg caggcgcgca gcagcgtgat
    12241 tccgatgaga agacattgct ggagtcgctc cggccagcgc tggcgctgct gtcgcaactg
    12301 aatacagcgg atcgcgacgc ggtgcgcgag gtcgtcacga cgctcacaca agggatggag
    12361 ttcgaccttc gaatctttcc gcctgaatcg tcggggcaac tggccgcgct cgacgaatat
    12421 tcggatctgg acagctacac gtatttcgtt gcagggtgtg tcggcgagtt ctggacgagg
    12481 atgacctggg cacacgcgcc gggcagcctt gccggggagc ccgaggcgat gctggaacgc
    12541 ggagtgcgtt ttggcaaagc gctgcaattg acaaacatcc tgcgcgactg cggcaaggat
    12601 ctgcgcatcg ggcgatgcta tttaccatcg tcgatcctcg cgaggtacgg gcttactccg
    12661 aacgatctac ttcggccgga aaactcccaa cgtacgcgac ccctgctgtt tgagctggta
    12721 cggcgcgacc tcgaccacta tcgcgctgca atggactaca cgctggcgtt acggccctcg
    12781 tcgaggcgat tgcgcctggc ctgcctgtgg ccggtgctga tcgggctgga gactatggct
    12841 ttgctggtgc ggaacgaggc gtggctggat cctgcaaagg catcgaagat ccggcgcagc
    12901 gacgtctaca aaatcgtggc gcgctccctc gtactggcgt cgagcaacag gcgattgaag
    12961 ctctggatgg acagcctcat cgcaaaagtg gaggagggga tgcagagcta gcgagcattc
    13021 cctcccgtct cttaccagac cttgcagact tttgcgcgga ccatgggctg accggctttg
    13081 cagccgaagg cctgcgcgaa ttgcggcagg ttggagacga taccgttgac acggtatttg
    13141 cctggcgagt gcgggttggt gatggcgctg acccgaaggt tctccgggcg ctcattctcg
    13201 cacgcccact gcgcgtagcc gacaaagaaa cgctggtcgg gcgtgaagcc ctctttgttt
    13261 tcgaggtgca tgccggcagt ttgcttcttc caggcaagat atgcgagcag cgtgccgcca
    13321 aggtcggcga catcttctcc gctggtcagc tttgagttga tgtggatgtc gtcgacgatg
    13381 acgtaagtcg catactgttc acgctggcag ttgatacggt cttcgaagcc cttcgcgtct
    13441 gcagcggtcc accagtcgcg caggttgccc tggccgtcga actggcggcc ttcgtcatcg
    13501 aagccgtggg tgagctcgtg gccgatcgtg ccgccggtgt cgccgtagtt cggcgcatca
    13561 tcctctttag cgtcgtacag cggcggctgc aggacaccgg ccgggaaagt gatgtcgttg
    13621 atttgcgggt cgtaacccgc gtttacggtg ggtggtgaca tgaaccactc gcttttgtcg
    13681 acaggcttgc cgaccttggc ccagtcgcgc ttctcctcaa aggcacgggc gcggagaacg
    13741 tttccgaaat agtcgtgagg atcgatggtg agcgaggaat agtcgcgcca cttatcgggg
    13801 tatccgatct tgtttcttac ctgatgcagc ttgacgatgg cggcctgttt ggtggcgggg
    13861 ctcatccagt cgaggttctg gatttcctcc tgcatggcct gctcgatttg ctgcgtcatg
    13921 accaggactt tctgcttggt atccggggag aacgtgcggg caacgaattc ctggccgaga
    13981 gcctcaccca tcgacctgtc gacctggccc tcacagcgtt tccagcgcgc cggcatttgc
    14041 ggaacgccgc ggagatactt cgagtagaag tcgaaccggg cctcttcaaa attgctcgaa
    14101 agactggaag cggcgaggtt gagtgcgtga atgcgcagat aggccctgag gctgcccaga
    14161 ggctccgtgg cgagctcagt ctgcagggcg gcaaggaact tcggctgagc gacgtcgacg
    14221 ttcgtgagta ccgcttcggg gaccttctgc ttcttcagat aagcaccgag ttcgagcgct
    14281 ggaacgaggg agtcgatcgc ctcgaccttc atcgggtgat aggtgttgta ggggtcgcgg
    14341 cgctccaccc gcgtgagcga agcgttcgcc agtgcagttt cgatcttcat ggtggccgcg
    14401 gcgtcccgat ctgcgtcggt ttgcggttcg ccggcgagga cgagcaggtg ggccatgtag
    14461 gcgacgaagt gctggcgaat gtcctgactg tgggcatcgg tcttcgtgta gtaatcgcga
    14521 tccggcaagc cgagacctcc ggcctcgatg ccgacgatca tcgagccggc gttttttgga
    14581 tcgggctctg aaccggcacc cagaaaatag aggcctcgct gatctgcgcc gtcgcgcagg
    14641 aatgcggcga tctgtttgcg atcctggatc cctgcaatgc gatccagctc cggctgaagg
    14701 ggcgcaaggc cgcgctggtc gattgcattc gtgtccatgc aggccgcgta atagtcaccg
    14761 atcttttgct gtgcgggcgt gcgatgggtc agcttcgcat ctttttcaag gattccccat
    14821 agcaacttgc gattgtcgtc cgtgagtttg gcgtagacgt cccaggccgc ctgatcggga
    14881 gggatgggat tgttcttcat ccagccaccg cacacgtact ggtaaaagtc gacgcaggga
    14941 tcagcggcgc ggtccatgtc ggcgagatca aaacttggcg agtacggaat ggcgtcgagc
    15001 ggatgttcgg tgggaggggc agaagtcgat tgggcgaaac acgcggggaa aagcgcagcc
    15061 gccagagcca acggcagaag ccgatacgtc atgcaatgtt ctccgtccgg cattatagcg
    15121 actgatctgc tgtcaccgtc atgcatgaca ccagcgggcc gcaggacgtg acggacgagg
    15181 ggatacactc gaattcgtgg ccaacgaaac gtcgaaacct acggttttcc tgctggacgc
    15241 catgtcgttc atctttcgtg cgtaccacgc catgcagcgg tcgcggccaa tgtcgacgcg
    15301 aacgggcgtg ccgacggcgg cgacgtatgt attcatcaac atgctgaaca agctccgcaa
    15361 ggatttttcg ccggagtatt ttgccgcggt gttcgatgtg tcggggccgg tgttccgtga
    15421 cgagcgggcc cgcggaatga cggtgaagaa gtacagcgcg gagacccagc agttcgagac
    15481 ggttgaaacg gggggctaca aggcgaagcg cgaggcgatg ccgccggacc ttgctcagca
    15541 gttgccctat gtgcggcgtg cgctggaagc ctttcgcatt cccattctcg aggcccaggg
    15601 tttcgaggcc gatgatgtga tcggaacgct ggcgacgaag gcggcgacgg gcggccatcc
    15661 cgtgcttgtc gtctcgaacg acaaggacat gatgcagctg gtgaacgagc acgtgaagat
    15721 tctgaatccc gtaaaggaca acctgttgct ggaccgggag aaagtgatcg aaactctggg
    15781 cgtaccgccg gagaaggtgg tcgacgtgat ggcgctgcgt ggcgattcca tcgacaacat
    15841 tccgggagcg ccgggcattg gcgacaaggg ctcggtcgag ttgatccagc agttcgggag
    15901 cgtggaggcg gcgctggacc gcgcggaaga gatcaagcgg aaaacgtatc gtgagtcgtt
    15961 acagaataac cgcgaggccg tgctgctgag caaggagctg gtcacgattc actgcgaggt
    16021 gccgctggac ttcgatctcg gtgcgatgcg gacgcaggag ccggatgtcg cggcatgccg
    16081 ggcgctgttt acggagttgg agttcacgac cttgctgcgc gagctggctc cggcggttcc
    16141 tgcacacgca gtggagtatg tgctggaacc gaccgcggaa cagctagccg cattcatgaa
    16201 acaggcgcag aaagacggat tcgcgctggc gctgccggct tcggaattgc aagccgctgc
    16261 ggagcaggtg agcgagcagg aaggtgaggc cctggaagag aaagagccgg aactcaagac
    16321 gatgtccctg ctggacctgg tggaccaggc ggaggcaaag tgcgaaacgg ctcaggcagt
    16381 ggcggagtgc aggcttggcg tggccgcggg atcggatgga gcgctactgg ttcccctgga
    16441 tgcggtcaga acgttgctgg aggatgcgaa ggttccgaag cacgtgcacg acctgaaggc
    16501 ggcgctgcgg gtttgcgagg cgcacggtgt ggcgctgcag ggtgcaacgg atgatctgat
    16561 gctgtactcg tacctgatca atcccacgca cgcgacccac cgccttggcg atgttgcggc
    16621 acggtttgga ggaggacctc cgcacgagac gggagaccgc gcgctggccg aggcggcatt
    16681 tctgacagag gcgctgacaa agatgatgcg cgatgaggtc gccgcgctgg aggagcggcg
    16741 tgtttatgac gagatcgatc tgcctctggt tccagtgctg ctgggcatgg agaaggaagg
    16801 cgtacggatc gattcggcag agctgtcgaa gatgtcggct gggctctcga aacagatgga
    16861 tcggctcagc gaggagattt tcgcgctcgc cggtcagcgc ttcaacatca attccccgaa
    16921 acagctgggc gatgtgctgt tcaacaagat gggactgccg cgtccgctga agtacggcaa
    16981 gggcaaggtg atttcgacgg cgcaggatgt gctggaggaa ctggcggtcc ataacgatgt
    17041 gccgcgactg gttctggagt ttcggcaact ggcaaagctg aaatcgaact actccgattc
    17101 cctgcccctt ttggccgatt ccgagggccg ggtccacacg acgttcaatc aggtgggcac
    17161 ggcgaccggg aggctgtcgt ccaccaatcc aaatctgcag aacattccga tcaagacgga
    17221 gctgggacgg gagattcgag cggcgttcat tgcggcgccg ggctgcgtgt tgctgtcagc
    17281 ggactactcg caaattgagc tgcggctcct ggcacacttt tcagcagatc cgctgctggt
    17341 gaaggcctat cagacagagc aggacatcca cacactgacg gccgcggagg tgtttggagt
    17401 gccggccgag acgatgagca aggagacccg taatcgcgcg aaggcggtga acttcggaat
    17461 tgtctacgga atttcgccct ttgggctggc tgcgcaactg ggaatcgatc agcatgaggc
    17521 tcggctgtac atcgagacgt acttcgaacg ctatgccggc gtgcgggtgt atatcgacag
    17581 aatgctcgaa gagacgcggc gcgagcagaa ggtgcggacc atgttcggcc gggtgcggcc
    17641 gattcccgac atccagagcc gcaatgccaa tcagcgcggg tttgcggagc ggacggcgat
    17701 caacacgccg ctgcagggca cggcggcaga tctaatcaag ctggcgatga tccggatcga
    17761 caagcgcctg gcgaaggaga agctaaaggc gcggatgact ctgcaggtgc acgacgagct
    17821 ggtgttcgat gtgccggagg atgagattga gccaatgaag gaactggtac ggcacgagat
    17881 ggagcacgtg gtggaactga gcgttccgct ggttgcggat gtgggcgtgg ggaagaactg
    17941 gcgggatctg gagtagccag ggaacgttac ttttcggtgg gatagtggta atcggcacgg
    18001 ctcaagaaga gtatctgccg acttagactg ggcccgtctt cttacaaagg gtgcacttgc
    18061 ggcaatgccg gagatcggcc cggagcattg ggctggcggt ccgcgagccg cttcgacgac
    18121 ctgcgggccg ttccgatgaa ggaggcaggc ccatggacaa aagaccgtcc tggacacgaa
    18181 tcgccattac agtcgccatt gactggcgcc ttgtaatggc gatcgtgatc cttgtggtcc
    18241 ttgtgctgct gatgaagtag catcaacttc ccgggagggg gcaccccttc cgggaactac
    18301 ttcgagacca gtatagctct gatcggcacg agtgccggcc attcgagggt gccgattgtt
    18361 ccgttttggg acgggccgcc taggcgccca gttcagctcg gatcgcatcg gcgatgcggt
    18421 cggcatggcg gcgcatggct tcttccgact ctgcttcgat catgacgcgc gccagggctt
    18481 cggtgccgga atagcgaacg accacgcgcc cgctttccgc cagctcgacc tcggcggcac
    18541 gaatggtatc cgcgacggtt gcaatttgat cgagaggctt tttttcgcga acgcgaacgt
    18601 tgacgatgac ctgcggaaag accttgagat cagcgatcag ttcgtgaagc gcgcggccgg
    18661 agcggcgaat gatatcgagg acgacgagcg cggtcagcag gccgtcaccc gtcgtggcga
    18721 gatgcgggaa aaggatgtgt ccggattgct cgcccccgag ggaagcctgc tccttctgca
    18781 tgcgctcaag gacgtattta tcgccgaccg gggcgcggag catgcgaatg gcatcccgcc
    18841 gcagcgctgc ttcaagaccc atgttggaca tggtggtggc gacgacggtg tcattgcgga
    18901 gaaggccgcg ctctttcatg tcgcgcgcgg cgagcagcat aatcgcgtcg ccattgacaa
    18961 cgttgccgtg ggcgtcggca aacatggcgc ggtcggcgtc gccgtcgaag gtcacgccca
    19021 gatccgcgtt gagattcttc gtttcggcgg cgacgacttc aggatgaaga gcaccgcaat
    19081 ttgcgttgat gttgcggcca tctggagagg cgtgcgtgag gtggatggtt ccgccgaggt
    19141 gatcgaaaag ctcaggggca atgacagagg ccgcaccgtt cgcgcaatca aggacgacgc
    19201 gcaaaccact gaggttcagg tccggaaatg cagagcgcag gaactgctca taatctttgc
    19261 gatatctgtc gttgacggcc ggagcggagc ccgaggcggg aacctgcgct tgttccagcc
    19321 gccgaaagat ctctgcttcg atgcgaagct cggtttcgtc gggtagtttg tagccgtcac
    19381 cgccaaagac cttgatgcca ttatcctgcc agggattatg cgaggcggag atgacaatgc
    19441 cggcggagaa tccgtgctgg cgcgccagat aagcaatgcc gggcgttgtg ataacgcctg
    19501 cgttctcgac ggcagcgcca ccttcggtca atccggcggc gatgatcgca gcgatccagc
    19561 tggaggactc gcgcgtatcc atgccgagga gaaccctcgg ttgcgcatgc gagccgttga
    19621 gctgatgggc gagcgccagg ccaaccgcat aaatcgtctt cgcgtccagc ggcgcttcgc
    19681 cggcgaccgc gcggatgccg tccgtaccga acagctttct catgagccct gtgtttcttt
    19741 ctcgaccgcc gtgacccggc tgcgaatgcg accgcgagcc aaccgggcaa cgatcgactg
    19801 accttgtgtg atgttctctg tatcttttat cagcatcccg cgttcgtcgt acaccagcgc
    19861 gtagccgcga ctaagaacgg cgagcggcga caggctttgg agctgacggg cgatcgccga
    19921 ctcccggtcg cgggaagtac ggaaccgggc gcgctgggcg cggatcatgc gcgcttcaag
    19981 ggcggtgagg cgttcgcgcg caatgccggt gcgggcgatg acatcctgcc gctggagccg
    20041 ggccgcgagg tgatgcagct tgtccgagag acggcggtgg cgaaggtacc actgcgattc
    20101 cagccgaagg gcaaggtcgt cgacacgctg ttgccggcgg cggaggtggt cgcgctcgcg
    20161 aatcaggatc gcttcgacgc gaactcgggc catggcctgc gaggactgca tgagccgata
    20221 gcgggcagcg cggacgaggc gctgatcgag agcctgaacc tgctccgcca cgttgtgcaa
    20281 tgcagcggtc acaatttccg cggcggcgga cggcgtgggg gcgcgcaggt cggccacgaa
    20341 atcggcaatc gtgaaatccg tctcatggcc gacggcggag atgaccggaa tctgcgagtt
    20401 gaaaatggct tcggcgagga attcggaatt gaagggggcg aggtcttcga gcgagccgcc
    20461 gcctcgcgcg atgacaatca cgtctacgct tcgggaacgg ttgaaatgcg cgatgccggc
    20521 ggcgacttcg gctgcagcag cctcaccctg gaccagagcg ggatagagca gaacgtgcag
    20581 gccggcgtgg cggcggttga cgatgttgat gaagtcgcgg atgacggccc cggttggcga
    20641 ggtaacgatg ccgacgcagt gcggaaaggc aggcagaggc tgctttgcgg cgggatcgaa
    20701 gaagcccctc gtttcgagcc gctggcggag ctgttcaaaa gcgatctgca gagacccggc
    20761 gccgacgggt tcgaggaatt ctgcgacgag ctgcatctgt ccgcgctgct cgtagacgga
    20821 gattttgccg cgcagcagga catgcaggcc gtcggcgggc ttgaa
//
LOCUS       DASZYN010000200         9221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_390286, whole genome shotgun sequence.
ACCESSION   DASZYN010000200 DASZYN010000000
VERSION     DASZYN010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 9221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9221
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390286"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VHE33_05145"
     CDS             complement(<1..215)
                     /locus_tag="VHE33_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96872.1"
                     /translation="MADLLASQARTTGGFWDKTRTWWDERLEKMLTVPGTAVWGEMKK
                     NADAISAGKGGAVMLYQAWKKAGLDPG"
     gene            complement(280..1443)
                     /locus_tag="VHE33_05150"
     CDS             complement(280..1443)
                     /locus_tag="VHE33_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HVT96873.1"
                     /translation="MPSSVRNAGKQAGKPTVHSKSKRRKNVVPDSIDLRDRVYNPSVT
                     RAPAEMFAPDIDIPVLDQKDTDACTGFGLASVIYHLQYRSKREKAVTPVSPFMLYSMA
                     RRYDEFPGDPNADTGSSLRGAMKGWFKHGACAASLWKTLAMPKPTRDPAKDWWQDAVT
                     RPLGAYYRIDIKSIADMQNALNEVGAIYASAACHDGWDTWKPSMKVAKGDYWTIPCQK
                     AKASDGGHAFAMVGYTRKGFIIQNSWDTSWATEGRAILTYQDWLDNAMDCWVAQLGVV
                     TELHDAIAAAPSLRTTSTGAVTLAVEETLRNREIDPFIVDMENNGLLSNTGDFRTQPS
                     DLEALITQHLSEARKKWGLGPTETVDIAIYAHGGLTSEGGCRGYGSQVDTRSL"
     gene            complement(1574..2317)
                     /locus_tag="VHE33_05155"
     CDS             complement(1574..2317)
                     /locus_tag="VHE33_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96874.1"
                     /translation="MGFLIHFIFTWWGLLLEALAIIHFIRRRPDGWWLFVIIFLGPLG
                     AIIYLLIEAAPDMRFLSQQSARGFSRRRRVRELEALVRVNTAVGNFEELGDLYMDEGR
                     LAQARACFDRSIQGGSTTIDPFYKRGACAVLMGDAAAAIPDLERVVTQEPGYDFFRAE
                     GLLAHAFAMTGQREKAETLFRAATQTSTMSETYLNFAELLASEGRTAEARDWARKLLN
                     KSQGMPAYLKRRERPWFRRARRFLSRLPA"
     gene            2477..3028
                     /locus_tag="VHE33_05160"
     CDS             2477..3028
                     /locus_tag="VHE33_05160"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVT96875.1"
                     /translation="MSIRTLSTREIYRNPWLRLREDQIERSNGTRGIYGVVEKDDCAT
                     IIPIEGDMIYLVEQFRYTIRQRALELPQGGWETADVDPEELARGELREETGLTAAKMT
                     RLGEMWIAYGFARQKEHVFLATGLTPGSTDPDPEEHDLVLRPTLIATFEQMILDGTIR
                     DECTIAAWGLYRLWAERQSTVSK"
     gene            3248..4654
                     /gene="tig"
                     /locus_tag="VHE33_05165"
     CDS             3248..4654
                     /gene="tig"
                     /locus_tag="VHE33_05165"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HVT96876.1"
                     /translation="MTAETTELNPAATAENEPTEHSHEGHQHEAHAHPHPTLNPDCTR
                     EIQVEAPADEVSKAFRTVLKRYQKLARIPGFRPGKVPESVLRSRFASALREDVVEALV
                     PEHFREAIAKQGVQPVSQPQVTDLQLQEGQPLTFKAVFEVLPQFSIDGYQDVKVEKPS
                     AELTDEEFNAELERIRDTHSRLEDVTEDRGLADGDWAKISFTGDVEGEAGSAGEAGES
                     ESSTPQPIEGRDVTVEIGGKDTVQAFTDALRGSKPGQELKFEVTYPGDFGQKRLAGKT
                     VAYKVEVKGIQTRVLPELNDEFAKELGHYESLEDFRQKLKEHLAADKKNRVFADTRNR
                     LVDALVSRFQFPVPESLVQSQIDARLERGLRALAAQGMRTEDMRRLDFDRLREAQRES
                     AIGEVKGTLVLDRVADVENIQISDEEVERELQVISLQTREPLETLRGRLEREGGLARI
                     REQLRREKTGSLLVERLS"
     gene            4735..5337
                     /gene="clpP"
                     /locus_tag="VHE33_05170"
     CDS             4735..5337
                     /gene="clpP"
                     /locus_tag="VHE33_05170"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: protein motif:HMM:NF001368.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HVT96877.1"
                     /translation="MALVPMVIEQTSRGERAYDIYSRLLRDNIIFLGTPIDDQIANLV
                     IAQLLFLSGEDPEKDIQLYINSPGGSITAGLAIYDTMQFIRNDCVTYCIGQAASMGAF
                     LLMAGTKGKRFALPNSRILIHQPSMGGLAGQATDIDIHAREIIRIREITNKLMAKHTG
                     QGLDKIERDVERDFIMNAPQAKEYGIIDEIIDRPRTTNSQ"
     gene            5380..6666
                     /gene="clpX"
                     /locus_tag="VHE33_05175"
     CDS             5380..6666
                     /gene="clpX"
                     /locus_tag="VHE33_05175"
                     /inference="COORDINATES: protein motif:HMM:NF003745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HVT96878.1"
                     /translation="MKTRTGPDEALRCSFCHKSQDAVAKLISSPSDYPRAYICDECVA
                     VCNSILEDDRTETTTGAAPTHLPKPLEVKAFLDEYVIGQDQTKKKLAVAVYNHYKRVQ
                     MNRTRGTDVELAKSNILLVGPTGSGKTLLAHTLAKMLDVPFAIVDATTLTEAGYVGED
                     VENIILKLLQAADGDVARAQTGIIYIDEIDKIGRKDENPSITRDVSGEGVQQALLKIL
                     EGTVANVPPQGGRKHPHQEFTPVDTTNILFICGGAFVGLERVIGRRVGKKALGFKAIA
                     DKDKEAQDPTGALHPQRDSELLRQAEPQDLIRFGLIPEFVGRLPVIGILDELDEVALV
                     EILTKPRNAILKQYQKLFDYEGVKLGFTEDATRAVAQEALLRKVGARGLRMILEELML
                     DLMYQLPSNKKVKELEIQAEMVRNRNLSMLLLDKAS"
     gene            6913..>9221
                     /gene="lon"
                     /locus_tag="VHE33_05180"
     CDS             6913..>9221
                     /gene="lon"
                     /locus_tag="VHE33_05180"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:NF008053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HVT96879.1"
                     /translation="MSQSSKESRERNDLRKFPMMPIRDMVIFPHMMTPFVVGRESSVR
                     ALEEALNGERKIFLATQHDARVDEPRPDEIYQVGTVGNIVQSVRMPDGNIKVLVEGIE
                     RARAVEINDTDGFFVATVKVAKPLPEITPQIEQLVQRVTSLFEQYVKLQQSLNVETVA
                     SAIRTDEPSKLADTIAANLQLEVPDKQELLDIFDLPERLLKIADVLDIEIEKLNMDRS
                     IQSRVKRQMERAQKEYYLNEKIKAIQKELGRGEKSEFDELKKKIETAGMPKDVFEKAI
                     QELKKLEAMPPMSAESTVSRNYLDWLLAVPWKKKSKETRSIDHAEKVLNADHYGLEKI
                     KERILEFLAVRQLVKNPKGSILCFVGPPGVGKTSLGMSIAKATGRKFVRMSLGGVRDE
                     AEIRGHRRTYIGALPGQIIQSMKKAGTRNPVFMLDEVDKMASDFRGDPASALLEVLDP
                     EQNHSFQDHYLDVEYDLSQVLFVATANVLHTIPPALQDRMEILRLHGYTEAEKLEIAK
                     QYLLKKQRDNTGLTEKNLAFTDPAITEMIRSYTREAGVRNLEREIGNVCRKVARRVVK
                     AGAKHKETVTPDNISDFLGVPKFRDSSVHEKSEVGLVNGLAWTEVGGSILQTEVQVLD
                     GKGKLTTTGQLGDVMTESAQAALSYVRSRAHHLGLPRDFYRNVDIHIHVPEGAIPKDG
                     PSAGITLATALASALAKIPVRRDLAMTGEVTLRGKVLAIGGLKEKLLAAHRAGIFEAI
                     LPEDNRKDLPDIPDNLRTAMKLNFVDSMD"
BASE COUNT         2055 a         2872 c         2639 g         1655 t
ORIGIN      
        1 ttccctgggt ccagccccgc ctttttccag gcctggtaca gcatcaccgc gccgcctttt
       61 cctgctgaga tcgcgtcggc gttcttcttc atttcgcccc aaaccgcggt cccgggaacc
      121 gtcagcattt tttccagtcg ctcatcccac caggtccttg ttttgtccca gaaaccaccg
      181 gtggtgcgcg cctgcgacgc cagcaaatcc gccatccggt ccttcagcgt cgacattaag
      241 tcggtctccc acatcaggaa caccggaaag atcttcttgt cataaagagc gggtatccac
      301 ctggctgccg tatccgcggc accccccttc cgaggtgagg ccgccgtgag catagatcgc
      361 gatgtcgacc gtttccgtcg gacccagacc ccacttcttt cgtgcctcgc tcagatgctg
      421 tgtgatcagc gcctccagat ccgacggctg cgtacgaaag tctccggtat tgctcagcag
      481 tccgttgttc tccatatcca cgatgaacgg gtcgatctcg cgattgcgga gtgtttcctc
      541 gaccgccagc gtgacggctc cggttgacgt cgtccgcagg ctgggcgctg cggcaatcgc
      601 gtcatgcaac tccgtcacaa cccccagctg cgccacccag cagtccatcg cattgtcgag
      661 ccagtcctga taggtcagaa tggcccgtcc ctccgtcgcc catgatgtat cccacgagtt
      721 ttgaatgatg aaacccttcc tcgtataacc caccatcgcg aatgcgtgtc ccccatcgga
      781 tgccttcgct ttttgacagg ggatggtcca gtaatcgccc ttcgcgacct tcatgctcgg
      841 cttccatgta tcccaaccgt cgtggcaggc ggcactggca tagatcgcgc ccacctcgtt
      901 cagcgcgttc tgcatatccg caatcgactt gatatcgatc ctgtagtacg cgcccaaagg
      961 tcgcgtcacc gcatcctgcc accagtcctt cgccggatcc ctggtcggct tcggcatcgc
     1021 cagcgtcttc cacaacgacg ccgcgcacgc cccatgcttg aaccatccct tcatcgcgcc
     1081 gcgcagactc gaccccgtat cggcattcgg gtcgcccgga aactcgtcat atctccgcgc
     1141 catgctgtag agcatgaaag gagaaacagg cgtgaccgcc ttttcccgtt tagaacgata
     1201 ttgcaggtga taaatgacgc tggccagtcc aaaacccgtg cacgcatcgg tgtccttctg
     1261 atccagcacc gggatatcga tgtctggcgc aaacatctca gcgggcgcac gcgtgaccga
     1321 agggttgtag acccgatcgc gaagatcgat gctgtcggga acgacgttct tcctgcgctt
     1381 gcttttgcta tgcacagttg gtttcccggc ctgcttcccg gcattgcgaa cagaggatgg
     1441 cataaaggcc tcccggtggt tcagttcgcc gtcgcgcgat aacccggaag atggatgggc
     1501 gctggcgtgg atgcaacata ccaccaggga ctcccggaag gaaccaacat tctctctgga
     1561 tccgcttttc gcctcaagcc ggcagccggc tcaggaaccg ccgcgccctc cgaaaccacg
     1621 gccgctctcg ccgcttcaga tacgccggca ttccctgcga cttgttcagc agtttgcgcg
     1681 cccaatcccg cgcctccgcc gtccgcccct ccgaagccag cagctcggcg aagttcagat
     1741 acgtttccga catcgtcgac gtctgcgtcg ccgcccggaa cagcgtctcc gccttctctc
     1801 tctggcccgt catcgcaaac gcatgcgcca gcaatccctc ggcgcgaaaa aaatcatagc
     1861 cgggctcttg ggtaacgacg cgttccaaat cgggaatcgc cgccgccgca tcgcccatca
     1921 gcaccgcaca cgccccgcgc ttgtagaaag gatcgatcgt cgtcgatccc ccctggatcg
     1981 accggtcgaa gcaggcccgc gcctgcgcca gccgcccctc atccatataa aggtcgccca
     2041 gctcctcaaa gttccccacc gctgtattca cccgaaccag cgcctccagc tcccgcaccc
     2101 gcctccgccg cgagaacccc ctcgccgact gctgcgacag gaatctcata tccggcgccg
     2161 cttcaatcaa cagataaatg atcgcgccca gcggccccag aaaaatgatg acgaacagcc
     2221 accacccatc cggacgtcgc cggataaagt gaataatcgc gagggcttcc agcaacagtc
     2281 cccaccacgt aaagatgaaa tggatcagaa atcccatggt cagcattcag aataacggac
     2341 caaagcagcg aacaagcgcg atcgggccgc tgtcggaggg ttcgctaacc ggtttaaagc
     2401 cagccgcttc accagctgac cggaacttag ctgaccgctt taccagctga ccccttcacc
     2461 agctactctg gtccccatga gcatccgcac tctctccacc cgcgagatct accgcaaccc
     2521 ctggctccgc ctccgcgaag accagattga acgctcgaac ggcacgcgcg gcatctacgg
     2581 cgtggtcgag aaggatgatt gcgccaccat catccccatc gagggcgaca tgatttatct
     2641 ggtcgagcag ttccgctaca ccatccggca gcgcgccctg gaattaccac agggcggatg
     2701 ggaaaccgcc gacgtcgacc ccgaagagct ggcccgtggc gagctgcgcg aagagaccgg
     2761 cctgaccgcc gcgaagatga cgcgcctcgg cgaaatgtgg attgcctacg gcttcgctcg
     2821 ccagaaggag cacgtcttcc tggcaaccgg cctcacaccc ggcagcaccg atcccgatcc
     2881 tgaagagcat gacctcgttc ttcgccctac tctgatcgca accttcgagc aaatgatcct
     2941 cgacggaacc attcgcgacg agtgcaccat tgccgcatgg ggcctctacc ggttatgggc
     3001 cgagcggcaa tcaacggtat cgaagtaacg ccacagatca cagggttcat gccccaggcc
     3061 gcccttgcgt ttgccggaaa cctgctgcat ccgtaccata aagagaagac ggatcagcgc
     3121 attcttcccg gcacgccaac cgagcggcgg gacgcaccga tcaaatcact tcgccgctcc
     3181 ccttccgccc tgcgcaacgc tctacctgcc agagacgagt cgggaatgcg gccccggaag
     3241 gcacaccttg acagcagaaa ccaccgagct gaatcctgcc gcaacggcgg aaaacgaacc
     3301 cacggaacac tctcacgaag gacatcagca tgaggcacac gcgcatccgc atccgaccct
     3361 gaacccggac tgcacgcgcg agatccaggt cgaggccccc gccgacgaag tcagcaaggc
     3421 attccgcacc gtgctgaagc ggtatcagaa gctggcgcgc attcccggtt tccgcccggg
     3481 caaggtgccg gaaagcgtgc tgcggtcccg cttcgccagc gcgctgcgcg aagacgtcgt
     3541 cgaggcgctg gtgcccgagc acttccgcga ggcgattgcg aaacagggtg tgcagccagt
     3601 ctcacagccg caggtcaccg atctgcagct ccaggaaggc cagccgctga cctttaaggc
     3661 cgtcttcgag gtcctgccgc agttcagcat cgatggctac caggacgtga aagtggagaa
     3721 gccgagcgcc gaactgaccg acgaagaatt caacgcagaa ctggagcgca ttcgcgatac
     3781 ccactcgcga ctggaagatg tcacggagga ccgcggcctc gccgacgggg actgggcgaa
     3841 aatcagcttc acgggcgacg tcgagggtga ggctggaagc gctggcgaag ccggggaatc
     3901 cgaatcgtca acgccgcaac cgatcgaagg ccgggatgtc accgtcgaga tcgggggcaa
     3961 agacaccgtg caggccttta cggacgcgct gcgcggctcg aagcccgggc aggagctgaa
     4021 attcgaagtg acctatccgg gggatttcgg gcagaagcgt cttgcgggta aaacggtggc
     4081 gtacaaagtt gaggttaaag gcatacagac aagagttttg cctgaactca acgacgaatt
     4141 tgccaaagaa cttggccact acgagagtct cgaagacttc aggcagaagt tgaaggagca
     4201 tctcgcagcc gacaagaaga acagggtttt tgccgatacg agaaatcgac tggtagatgc
     4261 gctggtttca aggtttcagt tcccggtccc tgagtccctc gtgcaaagcc agatcgatgc
     4321 ccgcctcgag cgtgggttga gagcgctcgc ggcacagggc atgcgcacgg aagatatgcg
     4381 caggctcgac tttgatcgat tgcgcgaagc gcagcgggaa tccgctattg gagaagtgaa
     4441 aggcacgctg gtactggatc gtgttgccga tgtggaaaac attcagatca gcgacgagga
     4501 agtggaacgg gagcttcagg taatctcatt gcaaacgcgg gaacccctgg aaacgctgcg
     4561 cggaaggctc gagcgcgagg gcggtttagc aaggatccgc gagcaactgc gacgggagaa
     4621 aacgggtagt ctgctggtag aacggttgtc ctgaagcgcg caatcgggcg cgcgggtgat
     4681 tcctgtcaca gacgatgagg cggttaagcc tcagaactga gagtagagac aaagatggct
     4741 ttagttccca tggtcattga gcagacgagc cgcggcgagc gggcgtatga catttattcg
     4801 cgtctgctcc gcgacaacat tattttcctc ggcacgccga tcgacgatca gattgcgaac
     4861 cttgtcatcg cgcagctctt gttcttgtcc ggagaagatc cggagaagga catccagctg
     4921 tacatcaact cgccgggagg ttcgattacg gccggcctcg cgatttacga caccatgcag
     4981 ttcatccgca atgactgtgt gacgtactgc attggacagg ctgccagcat gggagcattt
     5041 ctcctgatgg caggcacgaa gggaaagcgc ttcgcgttgc ccaattcccg catcctgatc
     5101 caccagccgt ccatgggcgg actggctggt caggcaaccg acatcgatat ccacgcgcga
     5161 gagattattc gcatccgcga aatcaccaac aagctcatgg ccaaacatac cggccagggc
     5221 ctcgacaaaa ttgaaagaga cgttgaacgg gatttcatca tgaacgcccc gcaggcgaaa
     5281 gagtacggga tcatcgacga gattatcgat cgcccgcgga caaccaacag tcagtgattg
     5341 cctgactgaa ttccccctga ggtagaggag tagccgcaga tgaagacgcg cacgggaccc
     5401 gatgaagcct tacgttgctc gttctgccat aaatcgcagg acgccgttgc caagctgatt
     5461 tcatcgccaa gcgattatcc gcgcgcgtac atctgcgacg agtgcgttgc ggtctgcaac
     5521 tccatcctcg aagacgaccg gaccgagact accaccggcg cggcgcccac ccatctgccg
     5581 aaaccgcttg aggtcaaggc gtttctggac gagtacgtca tcggccagga tcagaccaag
     5641 aagaagctgg ccgtcgcggt ctataaccac tacaagcgcg tgcagatgaa ccggacgcgc
     5701 ggaaccgatg tagagctggc gaaatcgaac attttgctgg tcggtcctac aggcagcggc
     5761 aagaccctgc tcgcccatac gctggccaag atgcttgacg tcccgttcgc aattgtggat
     5821 gcgacgaccc tcaccgaagc cggctacgtc ggcgaggacg tcgagaacat catcctgaag
     5881 ctgctccagg cggcagatgg cgatgtcgca cgggcccaga ccggcattat ttatatcgac
     5941 gaaatcgaca agatcggtcg caaggatgag aacccctcca tcacccgcga cgtttcaggc
     6001 gaaggcgtcc aacaggcgct gctgaagatt cttgaaggaa ccgtagccaa cgtcccgccc
     6061 cagggcggac ggaaacaccc ccaccaggaa tttacgccgg tggatacgac gaacatcctg
     6121 ttcatctgcg gcggggcgtt tgttggcctc gagcgggtga tcggacggcg cgtcggcaag
     6181 aaggcactgg gctttaaggc gattgctgat aaggacaagg aggcgcagga tccgaccggc
     6241 gccctccatc cgcagcgcga cagcgaactg ctgcgccagg cggagccgca ggatctgatc
     6301 cgcttcggac tcattccgga gtttgtggga cggctgccag tgatcggcat cctcgacgaa
     6361 ctggacgaag tggctctggt ggagattctc accaaaccgc gcaacgcgat cctcaagcag
     6421 taccagaagc tgttcgacta tgaaggcgtt aagctcggct tcaccgaaga tgccaccagg
     6481 gccgtggcgc aggaagcgct gctgcgcaag gtaggggccc gtggacttcg catgatcctc
     6541 gaggagctga tgctcgacct gatgtatcag ctgcccagca acaagaaggt gaaagagctg
     6601 gagatccagg ccgagatggt ccggaaccgg aatctttcga tgctgctgct cgacaaagcc
     6661 agctgatgtg cctttgtccg gagcgggata atttccaccc gtccggaccg atggctgcac
     6721 catgacggaa ccacacacgc cgccaactcc ccggggcatg gttaccgatg atctgccagc
     6781 ttgcaaaccg actcccccgt aacgccaaac atggccgcgg ggtgcgcagc cgttcgctga
     6841 atgggttact cttctcttaa gagcagaacc cgctacaatc attcgcaaga cagcagcctc
     6901 gaaaggaatc ccgtgagcca gtcgtcgaaa gaaagccggg aacgaaacga tcttcgcaag
     6961 ttccccatga tgcccattcg ggacatggtg atctttcctc acatgatgac cccgttcgtg
     7021 gtgggtcgtg aatcgagcgt gcgagccctg gaagaggccc tgaacggcga gcggaagatc
     7081 ttcctcgcca cccagcacga cgcccgcgtc gatgagcccc gccctgacga gatttaccag
     7141 gtcggcaccg tcggcaacat cgtgcagagc gtgcgcatgc ccgacggcaa tatcaaagtc
     7201 ctggtcgagg gaatcgaacg agctcgcgcg gtcgaaatca acgataccga cggtttcttt
     7261 gtcgcgaccg tgaaagtcgc caagccgctg ccggagatta cgccgcagat cgagcagctc
     7321 gtgcagcgcg tcacttccct gtttgagcaa tatgtaaaac tgcagcagtc tctgaatgtg
     7381 gaaacggtcg cttccgcgat tcgaaccgat gagccttcga agctggcgga caccatcgcc
     7441 gcgaatctgc agctggaagt gcccgacaag caggaattac tggatatttt cgatctgccc
     7501 gaacggcttc tgaagatcgc agacgtgctc gacatcgaga tcgagaagct gaacatggac
     7561 cgcagcatcc agtcgcgcgt gaagcggcag atggagcggg cgcagaaaga gtactacctc
     7621 aacgagaaga tcaaggcgat ccaaaaggaa ctgggacgcg gcgagaagag cgagtttgac
     7681 gagctgaaga agaagatcga aacggcgggt atgccgaagg acgttttcga aaaggcgatc
     7741 caggagctga agaagctcga agccatgccc ccgatgtcag ccgagtcgac cgtttcacgg
     7801 aattatctcg actggctgct ggccgtgccc tggaagaaga agtccaagga aacacgcagc
     7861 attgatcacg ccgagaaggt cctgaatgcc gatcactacg gcttggaaaa aatcaaagag
     7921 cgcatcctcg agttcctggc cgttcgccag ctcgtgaaga atcccaaagg ctcaatcctg
     7981 tgctttgtcg gacctccggg cgttggcaag acctcactgg gcatgtccat tgccaaagcg
     8041 acgggacgca agttcgtgcg catgtcgctc ggcggtgtgc gcgatgaggc cgagattcgc
     8101 gggcaccgtc gcacctacat cggcgccctg ccgggacaga tcattcagtc gatgaagaaa
     8161 gccggcaccc gcaatccggt tttcatgctg gacgaagtgg acaagatggc ctcggacttc
     8221 cggggcgatc cggcgtcggc gctgctcgag gtactcgatc cggagcaaaa ccacagcttc
     8281 caggatcact acctcgatgt ggagtacgac ctgtcacagg ttctcttcgt cgcgacggcc
     8341 aatgtgctgc atacgatccc gcccgcgctc caggaccgca tggagatcct ccgcctgcat
     8401 ggctacacag aagcggaaaa actggagatt gcgaagcaat atctcctgaa gaagcagcgc
     8461 gacaacaccg ggctaacaga aaagaatctc gcctttaccg atcctgcgat cactgagatg
     8521 atccgcagct acacgcgcga ggccggcgtc cgcaatctcg aacgcgaaat cggcaacgtt
     8581 tgccgcaaag tcgcgcggcg cgtggtgaag gccggcgcga agcataaaga aacggtaacg
     8641 cccgacaata tttccgactt cctcggtgtg ccgaagttcc gcgactcttc ggtccacgag
     8701 aagagcgaag tcggcctggt gaacgggctg gcctggaccg aggtcggcgg cagcatcctc
     8761 cagaccgaag tgcaggtgct cgacggcaaa ggcaagctca ccaccaccgg ccagctcggc
     8821 gacgtgatga cggagtcggc gcaggcggcg ctctcgtatg tgcgcagtcg cgcccatcat
     8881 ctcggcctgc cgcgcgattt ttatcgcaac gtcgatattc acattcatgt gccggaaggc
     8941 gcgattccca aggacggtcc gtcagcaggc attacgctgg ctaccgcgct agcgagcgcg
     9001 ctggcgaaaa ttcccgtgcg ccgcgacctg gcaatgactg gcgaagtgac gctgcgtggt
     9061 aaggtgctgg ccatcggcgg actcaaggaa aagctgctgg cggcacaccg cgccggcatc
     9121 ttcgaagcga tcctcccgga agacaatcgc aaggaccttc cggatattcc cgacaacctc
     9181 aggaccgcca tgaagctgaa ctttgtggat tcgatggacg a
//
LOCUS       DASZYN010000201         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_8331, whole genome shotgun sequence.
ACCESSION   DASZYN010000201 DASZYN010000000
VERSION     DASZYN010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8331"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VHE33_05185"
     CDS             <1..414
                     /locus_tag="VHE33_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96880.1"
                     /translation="EQSGLYKWSSLRSDYLAQANNQLAAQYGEGYAPGWYWDPWMTGY
                     TFLGPSPFYSPFGWGFYPFGYAGGYWGGYYPHVVHVYPPAPVHHRGFPGYRGPAFQSG
                     RAGGFRGGAFGGGGHLGGFHGGASSGGFHGGGRGH"
     gene            623..1189
                     /locus_tag="VHE33_05190"
     CDS             623..1189
                     /locus_tag="VHE33_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96881.1"
                     /translation="MRNLCGWSVVVTVLFSVSGWGQQMALLDAPEPAASSSSLVAGGA
                     GGPAGSSSLTGGAGSAVLPVMRKPAADRRAGLTQTDWTLLGAAGALRLLDYTSTVKAM
                     SDPIHFREDQLPNALVHCRPGLGAFEASMVGVNYYAYRVLVEHRHRKLARWGQVINLA
                     ATGWTVGRNYYELHEYWPRQNTLRPDSK"
     gene            complement(1201..>2301)
                     /locus_tag="VHE33_05195"
     CDS             complement(1201..>2301)
                     /locus_tag="VHE33_05195"
                     /inference="COORDINATES: protein motif:HMM:NF019583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HVT96882.1"
                     /translation="GWLPRWPAANDVTYVMGGDSPTILLADAYAFGARHFDAHAALQY
                     MLKAASQPGIGPHNESERPFLADELRLGYIPIDKDSIDGSRTLEYASADFAASQFARA
                     VGGDDLSAHALMNRAGNWQNLFDPETKWIRPRNSDGSWLKGFDPAHSLPKRDNAPVST
                     DQLGFEEGNTWQYSFMIPFDYPRLIAAMGGSAAVVPRLDKFFSKLICWGEPCFNMANE
                     PDFVTPYVYEYTDQPWKTAEVIARIEQQTFSTKPDGIPGNDDLGATSGVFVWNALGLY
                     PGVPGVGGFFLGTPMFPSVTVHFGNGRTLIVTSEGTGPYVRAITLNGQPLNQLWLPLD
                     KIPENATTTLHFVLQPDAPTATSFEPPPNFRP"
BASE COUNT          415 a          692 c          744 g          450 t
ORIGIN      
        1 gagcagagtg gcctgtacaa gtggagcagt ttgcgctcgg actacctggc ccaggccaac
       61 aaccagctgg ccgctcagta cggagagggc tacgcgccgg gctggtactg ggatccgtgg
      121 atgaccggat acaccttcct cggcccgagt ccgttctaca gcccgtttgg ctggggcttc
      181 tatcccttcg gatacgcagg cggatattgg ggcggctatt atccgcacgt tgtgcatgtt
      241 tacccgccgg cgccggtcca ccatcgtgga tttcctggtt atcggggccc ggcgtttcaa
      301 tccggccggg ccggtggatt ccgaggcggt gccttcggcg gcggtggaca tctcggcggc
      361 ttccacggtg gcgccagctc cggcggattc cacggcggcg gacgcggcca ttaagaactt
      421 cttagcttga acgagcgagc ctcgcatatt ccgatgcgag gctttttctt ttcggcagga
      481 tttcgcggga gttgagccga tcgcaatgaa aactgtgccg ccggtaacgc tcttccgtgc
      541 cgcgtttccc gatccgaaaa aggagtgatt cgaattagac ccccctcatc ttctgcagca
      601 cttactccga tgcacaaggg gtatgagaaa cctgtgcgga tggtcggtcg tcgtgacggt
      661 attgttctca gtcagcggtt ggggacagca gatggcgctc ctggacgcgc ccgaaccggc
      721 tgcatcgtca tccagcctgg tggctggggg cgcgggtggt ccggccggat cctccagtct
      781 gacgggaggc gcaggctcgg cagttctgcc ggtcatgcgg aaaccagcgg ccgatcggcg
      841 cgcaggcctg acgcagaccg actggacgct cctcggggcc gcaggggcgc tgcgcttgct
      901 cgactacaca tcgacggtaa aggccatgtc ggatcccatc cacttccgcg aagaccagtt
      961 gcccaacgcg ctggtccatt gccgtccagg gctgggtgcg tttgaggcga gtatggtggg
     1021 cgtgaattac tacgcctatc gggtcctcgt ggagcatcgg caccgcaagc tcgcgcgatg
     1081 gggccaggtc atcaatctag cggcgacggg ctggactgtc ggccgcaatt actacgagct
     1141 gcatgaatac tggccccgcc aaaacactct gcgccccgac agcaagtagc cgattaacga
     1201 ttacggccgg aaatttggcg gcggctcgaa gcttgttgct gtgggtgcgt cgggctgcag
     1261 aacaaagtgc agcgtcgttg tggcgttttc cggaatcttg tccagcggaa gccagagttg
     1321 gttcagaggc tggccgttca gtgtgatggc gcgaacgtac ggcccggttc cctccgatgt
     1381 gacgatgagc gtgcgcccgt tgccaaagtg cactgtcacc gacggaaaca tcggcgtgcc
     1441 caggaagaat ccacccacgc ccggcactcc tggataaagg cccagagcat tccacacgaa
     1501 tacgcccgac gtagcgccca ggtcgtcgtt gccgggaatc ccatccggct tcgtcgaaaa
     1561 cgtctgctgc tcgatccgcg cgatcacttc cgcggtcttc cagggctgat cggtgtactc
     1621 gtaaacgtag ggcgtcacga agtccggctc gttggccatg ttgaagcacg gttcgcccca
     1681 gcaaatcagt tttgagaaaa atttgtcgag ccgcggcacc accgccgccg acccacccat
     1741 tgccgcaatc agccgtggat agtcaaacgg gatcatgaac gagtactgcc aggtgttgcc
     1801 ttcctcaaag ccgagctgat cggtggagac gggagcattg tcgcgcttcg gcagcgagtg
     1861 cgccggatcg aaccccttga gccacgagcc atcggagttg cgagggcgga tccacttcgt
     1921 ctccggatcg aacaagttct gccagttccc ggctctgttc atcagtgcgt gagcgctgag
     1981 gtcgtcgccg cccacggcac gcgcgaactg cgacgccgca aagtccgcgg aggcgtactc
     2041 gagtgttcgc gagccgtcga ttgaatcctt gtcgatgggg atgtaaccga gcctgagctc
     2101 gtcggccaga aacggccgct cggattcgtt gtgcggacca ataccgggtt gcgaggcggc
     2161 cttgagcatg tactgcagcg cagcgtgggc atcgaagtgg cgcgcgccaa aggcgtacgc
     2221 atcggcgaga aggatcgtcg gcgagtctcc gcccatcacg taggtgacgt cgttggcggc
     2281 cggccagcgt ggcagccatc c
//
LOCUS       DASZYN010000202         1561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_8374, whole genome shotgun sequence.
ACCESSION   DASZYN010000202 DASZYN010000000
VERSION     DASZYN010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8374"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VHE33_05200"
     CDS             <1..903
                     /locus_tag="VHE33_05200"
                     /inference="COORDINATES: protein motif:HMM:NF005071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVT96883.1"
                     /translation="PHRDAQGHVDNAVLILHGTGGTGAQFLAPYFAGVLFGPGQLLDA
                     TKYYIILPDDIGHGGSSKPSDGMHAHFPQYDYDDMVAGEHLLLTEGLHVDHLRLAMGT
                     SMGCMHAWVWGETWPGFADALMPLACLPVQIAGRNRMMRKMAMDAITDDPAWNHGEYT
                     APPPGLRTSLEMLLIMGSSPLQMQEHYGTRDEADAYLKSFFDSRFKTTDANDFLYQFN
                     SSRNYDPSANLEKIQVPVMAVNSADDFINPPELDIMPKMIQRVPKGKYVLLPITEETR
                     GHGTHTHAAIWEKYLAELLQESAR"
BASE COUNT          321 a          420 c          517 g          303 t
ORIGIN      
        1 ccgcatcgcg acgcccaggg gcacgtcgat aacgcggtgc tgatcctgca tggaacgggc
       61 gggacgggag cgcagttcct cgccccttat tttgcaggcg tgctgtttgg gccgggacaa
      121 ctgctggatg cgacgaagta ctacattatt ttgccggacg atatcgggca tggtggatcg
      181 tcgaagccga gcgatgggat gcacgcgcat tttccgcagt acgactacga cgacatggtg
      241 gcgggcgagc atttgctgct gaccgaaggg ctgcatgtgg atcatctccg cctggcgatg
      301 gggacgtcga tgggatgcat gcacgcgtgg gtgtggggtg agacctggcc gggattcgcc
      361 gatgcgctca tgcccctggc gtgcctgccc gtgcagattg cgggaaggaa ccggatgatg
      421 cgcaagatgg ccatggacgc gatcacggac gatccggcat ggaatcacgg cgagtacacg
      481 gcgccgccgc cggggttgcg cacgtcgctg gagatgctgc tgatcatggg atcgagcccg
      541 ctgcagatgc aggagcacta cgggacgcgc gatgaagccg atgcctacct gaagagcttc
      601 ttcgactcgc gcttcaaaac gaccgacgcg aatgactttc tgtatcagtt caattcatcg
      661 cggaactacg atccttcggc gaatctggag aagattcagg tgccggtgat ggccgtgaat
      721 tccgcggatg actttattaa tccgccggag ctggacatca tgccgaagat gatccagcgc
      781 gtgccgaagg ggaaatacgt gctcctgccg attaccgagg agacgcgagg gcacggaacg
      841 cacacgcatg cagcgatctg ggagaagtat ctggcggagt tgctgcagga gtcggctcga
      901 tagaggcggc agccttttcc gtccagtgaa gagaggtcag gtgggcctga tggcctgtcg
      961 cagagcggtt gttaccggtg ttgaaagggc acagcttcac tgcatgcgga aagcccgaag
     1021 cgcggaaagg gtctggcttc agccggaccg taacgtctct ccactcggac ggaaccggaa
     1081 tccggcgggg agttttcctc cacccatgtg ctgcggagca gcacgacggt agaagccccg
     1141 gccttcaggc cggggaattc agagcttcaa agaaggggcc ttcaggcccg gtccctcctt
     1201 caccctgtca tcccgaccga agcggagtga cctgcttctc ttcagcaata agaaattgcc
     1261 gaaacaccac acgagtgtga tgtcccgcaa gttcgttcaa caaccgatcc tggttttgta
     1321 aaagggcacg acttcagtcg tgccacagag aacgcaaaaa ccaggggctt cagcccctga
     1381 ggcctggctc tccggcgact gattgttcaa tgaacttcat ggacagcaca ctagttgacg
     1441 gtgagggtaa gcgtcgtgct ggcggtaatg ccggaggttg tggtgtcggc cccgaccacg
     1501 gtgacgatgt agctgccggt ggagacctgg ttattcgtcg acgcggtgga gttggaggtc
     1561 g
//
LOCUS       DASZYN010000203        10486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_584382, whole genome shotgun sequence.
ACCESSION   DASZYN010000203 DASZYN010000000
VERSION     DASZYN010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 10486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10486
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584382"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VHE33_05205"
     CDS             <1..405
                     /locus_tag="VHE33_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96884.1"
                     /translation="GPHSQDICYATENRQVAVKNVAHSADLVLVVGSNNSSNSNRLVE
                     VSRNLGTQGYLIDNAEAIDPAWLKGVQNVAVTAGASAPEILVEGVIDYLKSNGFGTVE
                     EVEVMPENVRFGLPPEIVQAIQSAPPTTAAGV"
     gene            555..2528
                     /gene="shc"
                     /locus_tag="VHE33_05210"
     CDS             555..2528
                     /gene="shc"
                     /locus_tag="VHE33_05210"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HVT96885.1"
                     /translation="MKFGRIDAALEDVAHAIERSRNALLGMQQGEGQWCGELEGDSML
                     EADYIFLHVLLGTGDQGKMERALNEIMRYRNDDGSWSLYPGGPGNISLSVKCYFANKL
                     MGVSADEPHMVQTREWVLAHGGVVACNTFTKIYLCALGQYEYNSVPAIPPEIVLFPKW
                     FYFNIYEISSWSRGILVPLSIAYAKKPFKKIPAEHGISELFVGGRENADLSLRWDRDK
                     VVSWRNFFIFLDRVTHWFERIHIRPLRSIALKKAQKWMLERLEMSDGLGAIYPAILNS
                     IIALRCLGYSLDDPQVIRAMDEFEKLGVDAPNGTPDYPVPTFRMQPCMSPVWDTAQAV
                     YALGEAGLDRKDPRLLKAADWMLSKEVRHKGDWSVKNRRAAPGGWYFEYNNEFYPDVD
                     DTAQVLLALNKVDNPRERYQYDVCQRAIDWLLSMQCKNGGWGSFDKDNTKMIFQHIPF
                     ADHNAMLDPPTVDITGRILEMLATYGYTRKDRRVEKAIKFIYSEQEPDGSWFGRWGVN
                     YLYGTFLVLRGLEAMGVWSHEPEIQQAAEWIRSVQNADGGWGESCHSYDDPVTRGVGV
                     STPSQTAWGVLGLLAAGDNRSDSVAKGIKWLLAKQQSDGSWDESTGQGKTRQAIYTGT
                     GFPRVFYLAYPLYRDYFALLALTSYKRAMDKAD"
     gene            2664..3788
                     /gene="hpnH"
                     /locus_tag="VHE33_05215"
     CDS             2664..3788
                     /gene="hpnH"
                     /locus_tag="VHE33_05215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HVT96886.1"
                     /translation="MAVPVSQAWTVATYVLKQKLKGRKKFPLVTMLEPLFRCNLACAG
                     CGKIQYPPHILKQELTPEECFRAIEESGTPMVSIPGGEPLLHPQIDKIVEGLVARKKY
                     VYLCTNALLLKEKLHLFKPSKYLSFSVHLDGQREHHDFSVCREGGYDIAMEGIKAAVA
                     AGFRVTTNTTLFDGADPNSVRGHFDDLMNVGVEAMMVAPGYTYEKAPDQKHFLGRARS
                     RRLFRAILSNRKKSWKFNANYLYLEFIMGKRDFHCTPWAMPTFSIFGWQKPCYLLQDG
                     YADTFQELIDSTNWENYGTESGNPKCANCMVHSGYEGTAVNYTFGSLKGLWQTAKAMA
                     NLYPDKGALDLLNEPVKPAHSFNPLVQIETKVTVEEGTRV"
     gene            3785..4234
                     /locus_tag="VHE33_05220"
     CDS             3785..4234
                     /locus_tag="VHE33_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96887.1"
                     /translation="MSHQPLRGEPDAGTNGTTFDPNSVEVMEGRQREQLEGWIPSLAS
                     EGEIRQALEKAFDYRGDVTLTLKNGDVVHGYVFDRRPGTTLEDSAVRVMPSDQRTRLT
                     IPYSDIAALAFTGRDNAAGKTFEAWVRKYWEKKAAGEKNIGIEAEKL"
     gene            4350..5168
                     /locus_tag="VHE33_05225"
     CDS             4350..5168
                     /locus_tag="VHE33_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96888.1"
                     /translation="MRVRILSLFALLLCATWSFAQESNSTDPVDDAPVVTLAANQQSS
                     PAPQSTNNSQLPSAPQPANAPNAVAVPPSKQQTKRILGVMPNFRAVSQGTIPPPPGPK
                     RAFVIATENSFDYSAFVFVGVTSLLAEGSNAHPTLGKGIGGYWGYYWRGYLDKTDGNY
                     LVIWALPSLLHEDERYFTRGQGSILGRGVYAASRVLITPDYHGHNTANIAELGGRAIA
                     QAVSLSYYPSADRTFGDVAERYGYALGRDALTATFREFWPDIAAHVLPHHKKGN"
     gene            5461..6738
                     /locus_tag="VHE33_05230"
     CDS             5461..6738
                     /locus_tag="VHE33_05230"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HVT96889.1"
                     /translation="MNCGAAAQSSAAASNASFAEAVPTPIASPASANPAQPVVQPPVY
                     APAPGQAVYVQPNGQPMYYVPSGAAPHAHAGHQLNLLHGLQARLQSLASTEQLEGFSL
                     KQMFSEVFKRRSEDEIEDYLLVGTSRTTPPIDVCQTGWPKPWLFFRVLVILAVTYTVF
                     TITLLHLPDAGNLIPAVMFIGAFAVPLTTLAFFFEMNSPRNVSVHRIVTLFLYGAVVS
                     LIFALVGYELPILGSLGAIAAGVVEEVAKLLTVVVVIRGVKYKYILNGLLFGATVGAG
                     FAAFETAGYALNKGLLAGHDLASGVTNMLAVLSIRAFLTPWGHVAWTAIAAAALWRVK
                     GAQRLKPSMFFDSRFLKAFMIPVVLHIAWDCPWQLPFEGNAIISAVVSWYVVFGLVQQ
                     GLRQVKEEQKLQLQQTLQRVETSMQPVGSVAVS"
     gene            6784..7464
                     /locus_tag="VHE33_05235"
     CDS             6784..7464
                     /locus_tag="VHE33_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HVT96890.1"
                     /translation="MAFCSNCGHQLAGNEKFCAECGTVVQGSTPHATAATPAPITSPT
                     PSVPPIAAAPTAYPAGPVPVVVGAPPTQPLQQKKGMVGTVVIVLLAAAIGYYFYNRSH
                     APAKPAPTPAPVVQTPPPNGNGGNGGTAALVNLQSFNAHWQDESGMLTLTTATWANNS
                     TTDLASATLQCRQFNTAGTDLSEYRVTLNGPTKAQTSSQFSNISLGATASGMTKVDCS
                     IIHVKPTS"
     gene            complement(7660..8562)
                     /locus_tag="VHE33_05240"
     CDS             complement(7660..8562)
                     /locus_tag="VHE33_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HVT96891.1"
                     /translation="MAFCLLLLSVFTTLSTGAVLMYNFRRGAPPFAVNAGFFPFAWIV
                     RHPDLLSGGWSFALTLLGILAAHEIGHFFACRAHGVRSSLPWVLPAPTLIGTFGAFIR
                     LRSRVPSRMALLDIGVAGPLAGMVVAIPALISGLLLSQPTAAPGFQSSASISVSVPFA
                     LSIVHGMVRWFYPGVPPLDASFNPHPVLVAAWIGLFITSVNLMPAGQLDGGHILYAVS
                     PRVHRWATNTIVFLLFFAGTMFWVGWLVWGCFLLPSLKHPAVAEAPLTKGRMWLAFAG
                     LVLFALTFTLEPFAGGSLMNLLHN"
     gene            8754..9113
                     /locus_tag="VHE33_05245"
     CDS             8754..9113
                     /locus_tag="VHE33_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96892.1"
                     /translation="MAVEKVQFFPRLKANCLARGDADFGSGARIPANSGFAGTDVENT
                     EAAEFNPLTLCKSPLQALEDRIDSSLGLVALQAGAFDHLVNDVLLYQGFPPSGEVSVS
                     TLIVETFDGIVNVAAVP"
     gene            9231..9782
                     /gene="dcd"
                     /locus_tag="VHE33_05250"
     CDS             9231..9782
                     /gene="dcd"
                     /locus_tag="VHE33_05250"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HVT96893.1"
                     /translation="MAIKSDRWIREQALKGMIDPFSEKQVREGVVSYGLSSYGYDLRV
                     SDEFRIFTNVNSAVVDPKKFDERSFVTVQSESILVPPNSFALARSVEYFRIPRDVLTI
                     CVGKSTYARCGIIVNVTPFEPEWEGFVTLEISNTTPLPARVYANEGLCQILFFQSDEP
                     CEISYADRKGKYQKQQGIVLPKL"
     gene            9788..10447
                     /locus_tag="VHE33_05255"
     CDS             9788..10447
                     /locus_tag="VHE33_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT96894.1"
                     /translation="METGSHTTLKAKVGLLDFEPLRIAGLREIFAEQPDFQVIATDWA
                     SAFRDSALDLVVVVLRDQAFSLALLARLRTRHPGLKVLVIGTGSEPEKILEAIGAGAK
                     GWLEETASPEQVMHAACAILDGSIWAPRQVLATFVDRALEGSAARRRRSSPRFTEREE
                     EVLQQLVLARSNREIAQALSIREQTVKSYVARLMRKVGVGNRIALSVQAASGAWGEKK
                     L"
BASE COUNT         2186 a         3165 c         3136 g         1999 t
ORIGIN      
        1 gggcctcatt cccaggacat ctgctatgcg actgaaaacc gtcaggtcgc ggtgaagaat
       61 gtagcgcaca gcgccgatct ggtgctggtg gtgggttcga acaacagctc gaactcgaac
      121 cggctcgtcg aggtatcgcg caacctcggc acccagggct acctgattga taacgccgag
      181 gcaattgacc cagcctggct gaagggcgtc cagaacgtcg cggtcacagc aggggcgtcg
      241 gcgccggaaa ttctggtcga aggcgtcatt gactatctga agagcaatgg cttcggaacc
      301 gtcgaggaag ttgaggtcat gccggaaaac gtccggtttg ggcttcctcc ggagatcgta
      361 caggccatcc agtcggctcc gcccacaact gcggcgggag tctaacccag ggtttcgggc
      421 atcgcactga ggagagagct gttttctcgg acgatatgag tacatcactt ccagccgccc
      481 ggccaaagtc catcgcagag agtgccgacg gcccatctcg gggcgaaaat cagccggcca
      541 gtgcggctgc gcaggtgaag ttcggacgaa tcgatgcggc actcgaagac gtggcgcacg
      601 ccatcgagcg ttcccggaac gctttgctgg gcatgcagca gggtgaaggt caatggtgcg
      661 gggagctgga aggcgactcc atgctggagg ccgactatat cttcctccac gtgctgctgg
      721 gcaccggcga tcagggaaag atggagcggg cgctgaacga gatcatgcgc tatcgcaacg
      781 acgacggaag ctggtcgctc tatcccggcg gccctggaaa catcagcctg tcggtcaaat
      841 gctacttcgc caacaagctc atgggcgttt ccgccgatga gccgcacatg gtccagaccc
      901 gcgaatgggt tctggcgcac ggtggagtcg tcgcctgcaa taccttcacg aagatctatc
      961 tctgcgcgct cggccagtac gagtacaact cggtgccggc cattccgcct gagatcgtcc
     1021 tcttcccgaa gtggttctat ttcaacatct acgaaatttc ctcgtggtcg cggggcattc
     1081 tcgttccgct ttccattgcc tacgccaaga agcccttcaa gaaaattccc gctgagcacg
     1141 gcatcagcga actgttcgtt ggcggtcgtg agaatgccga cctgagcctg cgctgggatc
     1201 gggacaaggt tgtcagctgg cggaatttct tcatcttcct cgatcgcgtc acgcactggt
     1261 tcgaacgcat ccacattcgg cccttgcgct ccatcgcgct gaagaaagcg cagaagtgga
     1321 tgctcgagcg cctggagatg tccgacggtc tgggagcgat ctatccggcc atcctgaact
     1381 cgatcatcgc gctgcgctgc ctcgggtatt ccctcgacga tccgcaggtg atccgggcca
     1441 tggatgagtt cgagaagctc ggtgtcgacg cacccaacgg cacaccggac tacccggtgc
     1501 ccaccttccg catgcagccc tgcatgtcgc cggtctggga tacggcccag gctgtctatg
     1561 ccctcggcga ggcaggactt gatcgcaaag atccgcggct gctgaaagcg gcggactgga
     1621 tgctgtcgaa ggaggtccgg cacaagggcg actggtcggt gaagaatcgg cgtgcggctc
     1681 ccggcggctg gtacttcgag tacaacaatg aattctatcc ggatgtcgat gataccgctc
     1741 aggttttgct cgctctaaat aaggtcgaca atccccgcga acgctatcaa tatgacgtct
     1801 gccagcgcgc catcgattgg ctcctttcca tgcagtgcaa aaacggcgga tggggaagtt
     1861 tcgacaaaga caacaccaag atgatcttcc agcacatccc cttcgccgac cataacgcga
     1921 tgctcgaccc cccgacggtc gacattacgg gacggatcct cgaaatgctg gcgacctacg
     1981 gctacacacg caaggaccgc cgcgtggaga aggcgatcaa gttcatctac tccgaacagg
     2041 aaccggatgg aagctggttc ggacgctggg gtgtcaatta tctctacggc actttcctgg
     2101 tcctgcgcgg tctcgaagcc atgggcgttt ggagtcacga gcccgagatc cagcaggcgg
     2161 cagagtggat tcggtctgtg cagaatgccg acggagggtg gggagagagc tgtcacagct
     2221 acgacgatcc ggtaacgcgc ggggtgggcg tcagcactcc gtcgcaaacg gcgtggggtg
     2281 ttctgggtct gctggctgct ggcgataacc gaagcgactc ggtagccaag ggcatcaaat
     2341 ggttactggc aaagcagcag tctgatggtt cctgggatga aagcactggg caggggaaga
     2401 cgcggcaggc catctacacg ggaaccggct tcccgcgcgt gttttacctc gcctacccgc
     2461 tgtatcgcga ttatttcgcg cttctggcgc tgacgagcta taagcgagcc atggataaag
     2521 ctgattagcg gaacaccgcg agtgcaacgc cgttttgaaa gggcacggct tgagccgtgt
     2581 cggagcaaca aattctatcg ggctttagcc cctgagggat gcgttcccag gctggatctg
     2641 gacctcccgg aaggaaacac tgaatggcgg tacctgtatc gcaggcatgg acggtagcaa
     2701 cctacgtcct caagcagaag ctgaaaggcc ggaagaagtt ccctctggtg accatgctgg
     2761 agccgctgtt tcgctgcaat ctggcgtgcg ccggctgcgg caagattcag tatcccccgc
     2821 acatcctcaa gcaggagctg accccggagg agtgcttccg tgccattgaa gaatcgggca
     2881 cgcccatggt gtcgattccc ggcggcgagc cattgctgca cccgcagatc gacaagatcg
     2941 tcgagggcct ggttgcgcgc aaaaagtacg tttatctctg caccaacgct ctgctcctca
     3001 aagaaaagct gcacctcttc aagccgagca aatatctttc cttttcggtc cacctcgacg
     3061 gccagcggga gcatcatgac ttctccgtat gccgcgaagg cgggtacgac attgccatgg
     3121 aaggcatcaa ggccgctgtg gcagcaggct ttcgcgtcac gaccaacacc acgctgttcg
     3181 acggagccga tcccaactcc gtgcgcggtc acttcgacga cctgatgaac gtcggcgtcg
     3241 aggccatgat ggtcgccccc ggttacacct acgagaaggc tcccgaccag aagcacttcc
     3301 ttggccgggc acgctcccgc cgcttgttcc gcgccatcct gtcgaaccgg aagaagagct
     3361 ggaagtttaa cgcaaactac ctctacctcg aattcatcat ggggaaacgc gacttccact
     3421 gcacgccctg ggcaatgccc acgttcagca tcttcggctg gcagaagccc tgctatctcc
     3481 tgcaggacgg atacgccgat accttccagg aactgattga ctccacgaac tgggagaatt
     3541 acggaaccga gagcggcaat ccaaaatgcg cgaactgcat ggtgcactcc ggctacgaag
     3601 gcaccgccgt caactacact ttcgggtcgt tgaagggttt gtggcagacg gcgaaggcca
     3661 tggcgaacct gtatcccgac aaaggcgcgc tcgacctcct gaacgaaccg gtgaagccgg
     3721 cgcacagctt caatcctctg gttcaaattg agacgaaggt cacggtcgaa gagggaactc
     3781 gcgtatgagc caccagcccc tgcgcggaga gcccgacgcc ggcacaaatg gaacaacctt
     3841 tgatccaaat tccgtcgaag tgatggaagg ccgccagcgc gaacagctgg agggctggat
     3901 tccatcgctc gcaagcgaag gggagattcg ccaggcgctg gagaaggcct tcgactatcg
     3961 tggcgatgtg acgctgaccc tgaaaaacgg tgacgtcgtc cacggatacg tcttcgatcg
     4021 gcggcctggc accacgctcg aggattccgc cgtgcgcgtg atgccgtcgg atcagcgaac
     4081 caggctcacc attccatatt ccgatattgc cgcgctggca ttcaccggcc gcgacaatgc
     4141 agcaggaaag accttcgaag cctgggtgcg caaatactgg gaaaagaaag ccgccggcga
     4201 gaagaacatc ggcatcgagg cggagaagct gtaacatccg ccgacggaca cacgtgtccc
     4261 acaccaccct ctgcggaatt cccttagcct aagtttccat ttctacgtct aaccacgcag
     4321 gccttatccc ccctgttgga ggctctcttt tgcgcgttcg catcctctcc ctttttgcgc
     4381 ttttgctttg cgccacctgg tcctttgctc aggagtcaaa ttcgacggat ccggtcgacg
     4441 atgcacctgt cgttaccttg gccgcaaacc aacaaagttc gcctgcgcct cagtccacca
     4501 acaattccca actccccagc gctccgcaac cggccaacgc tcccaacgcc gtcgccgtgc
     4561 cgccatcgaa gcagcagact aagcgcatcc tcggagtgat gccgaatttc cgcgccgtca
     4621 gccagggaac catcccgcca ccgcctggcc cgaagcgggc cttcgtgatt gccaccgaga
     4681 acagcttcga ctattccgcg ttcgtgttcg tcggcgtgac ctcgctgctg gcggaaggct
     4741 ccaacgcgca tcccacgctg ggcaagggaa tcggcggcta ctggggctac tactggcgcg
     4801 gctatctcga caaaaccgac ggcaattacc tcgtgatctg ggcgctgccc agcctgttgc
     4861 atgaagacga gcgctacttc accagggggc agggcagcat cctgggtcgc ggtgtctatg
     4921 cggcgtcccg agtgctgatc actcccgact atcacggcca caatacagcg aacattgccg
     4981 aacttggagg gcgggccatc gcgcaggccg tctcgttgtc gtactatccc tccgccgatc
     5041 gcaccttcgg cgatgtagct gaacgttatg gttacgccct tggacgcgat gcgctgacgg
     5101 caaccttccg cgagttctgg ccggacatcg cagcccatgt gctgccgcac cacaaaaaag
     5161 ggaactgact tcgggaaccg tccacgcatc aacgagcgca atcaaagtaa caattcaccc
     5221 ttccccaggt aatgcgcgag gattccgcat gcagcagcgg actgctgacc cgcagcccac
     5281 tccgttgcgc ccatgggcat gggccacttc gcgcaatcgc gtcgcaccca tctttacctt
     5341 tacttcgaaa acgcggccgt gctacgttcc tataccaatc ccccgccaaa accgaagtgg
     5401 agaattcagg tgtactgcat tcagtgcgga tcctcgatcc cttccggaaa caagttctgt
     5461 gtgaattgcg gcgcggccgc gcagtcgtcg gcagcggcat cgaatgcctc ctttgctgag
     5521 gctgtgccca ctcccatcgc gtcccccgca tccgcgaatc cggctcagcc cgtcgtccag
     5581 ccgcccgtct atgcgcctgc gccaggccag gccgtttacg tgcagcccaa cgggcaacca
     5641 atgtattacg tcccatcggg agcggctcct cacgcccatg ccggacatca gctgaacctg
     5701 ctgcacgggc tgcaggcgag gctgcaaagt ctggcatcga ccgaacagct ggagggcttc
     5761 agcctgaagc agatgttctc agaggtgttc aagcggcgca gcgaagacga gattgaggat
     5821 tatctgctgg tgggcacaag ccgcaccacg ccgcccatcg atgtgtgcca gaccggctgg
     5881 ccaaagccgt ggctcttctt ccgcgtgctg gtgattcttg ccgtcaccta caccgtgttt
     5941 accatcaccc tcctgcatct gcccgatgcg gggaatctga tccccgccgt gatgttcatc
     6001 ggagccttcg cggtgccgct caccacgctg gcgtttttct ttgagatgaa cagcccacgc
     6061 aacgtctccg ttcacaggat cgtgacgctg ttcctctatg gagcggtggt gtcgctgatc
     6121 ttcgcgctgg tcggttatga gctgcccatt ttgggatcgc tgggcgcgat cgccgctggc
     6181 gtcgtggaag aagtcgccaa gctgctcacc gtcgttgttg tgatccgtgg cgtgaagtac
     6241 aagtacatcc tcaacgggtt gctgttcggc gctacggtgg gtgccggttt tgccgccttt
     6301 gagacggcag gctatgcgct gaacaaaggc cttcttgccg gccacgactt ggcttccggc
     6361 gtcacgaaca tgctggcagt gctgtccatc cgcgccttcc tcacgccgtg gggacacgtt
     6421 gcatggacag cgatcgcggc ggccgctctg tggcgcgtca aaggcgccca gcggttgaag
     6481 ccttcaatgt tcttcgactc acgattcctg aaggccttca tgattccggt ggtgctgcac
     6541 atcgcctggg attgcccctg gcagctcccg ttcgagggaa acgcaattat ctcggccgtg
     6601 gtcagctggt acgtcgtctt cggtttggtg cagcagggac ttcgccaggt gaaagaggag
     6661 cagaagctgc agctgcagca gacactacag cgcgtcgaga catcgatgca gcctgtcggc
     6721 tcggtcgccg tttcctgatc acgcgggtgg tcccccaggt catcgctcag gagaagatcc
     6781 gtcatggcat tctgcagcaa ttgcggtcat cagctcgcag ggaatgagaa gttctgtgcc
     6841 gagtgcggaa ccgtggtgca gggaagcacg ccccatgcaa ctgcggccac tcctgcgccg
     6901 ataacgtcgc cgactccgtc ggtgccgccc atagcagcgg ctccgacagc ctatcccgca
     6961 ggaccggtcc cggtcgttgt gggtgcgccg ccgacacagc cgcttcagca gaagaagggc
     7021 atggtgggca ccgttgtgat cgtccttttg gcggcggcca tcggctatta cttctacaac
     7081 cggtcgcacg ccccggcaaa gcccgcacca acaccggcgc ccgtcgtcca gactccgccg
     7141 cccaatggca atgggggcaa tggcggaacc gcggcgctgg tgaacctgca gagcttcaat
     7201 gcacactggc aggacgagag cggcatgctg acgctaacaa cagcaacatg ggcaaataat
     7261 tccaccaccg acttggcgtc cgcaacgtta cagtgccgcc agttcaatac ggccggtacg
     7321 gacttgtcgg aatatcgcgt gaccctcaat ggaccgacga aagctcaaac cagcagtcag
     7381 ttcagcaata tctctctggg cgcgaccgcc agcggcatga cgaaggtgga ttgctccatc
     7441 atccacgtga aaccgacgag ctaaaaggtg ggagtgctgg aatggaccgc atgagggttg
     7501 cccgcgcttt gtggtgtccc gcaggttcgt tgaagaatgg gccgacgctc ttgtaacagg
     7561 gcatgacctc agtcgtgccg caaaggcgca aaaatgatgt gggcttcgcc cctgaggctt
     7621 tctcttcagt cacctcttat ccattttcgg gacagcacgc tagttgtgca gcagattcat
     7681 cagactgccg ccggcgaagg gctccagcgt aaacgtcagc gcgaagagca cgagccctgc
     7741 aaaggcgagc cacatgcgcc cttttgtcag aggcgcttcg gcgacggccg gatgtttcag
     7801 gctcggaagc aggaagcatc cccacaccag ccaaccgacc caaaacatcg tcccggcaaa
     7861 aaacagaaga aacacgatgg tgttcgtcgc ccaccggtgc acgcgcggcg agaccgcata
     7921 aaggatgtgt ccgccgtcga gctggcctgc aggcattagg ttcacggagg taataaacag
     7981 tccgatccat gctgcgacca ggacaggatg cggattaaac gaggcatcca gtggcggcac
     8041 tccgggatag aaccagcgca ccatgccgtg aacgatcgac agggcaaaag gcacactcac
     8101 gctgatgctc gcgctggact gaaaacccgg agcggccgtc ggctgcgaca gcaggaggcc
     8161 gctgatcaga gcagggatcg caaccaccat gcccgccaga ggtcccgcga cgccgatgtc
     8221 gagcagcgcc atgcgcgacg gcacccgcga acgcagacgg ataaacgcgc cgaaggttcc
     8281 gatgagcgtc ggcgctggca atacccaggg cagagaagaa cggacgccgt gagcgcggca
     8341 cgcgaagaag tggccgattt cgtgggcggc gagaatgccg agcagagtga gtgcgaagga
     8401 ccatccgccg gaaaggagat cgggatgccg aacgatccag gcaaagggaa aaaaaccggc
     8461 gttcaccgcg aacggcggag ccccacggcg gaagttgtac atcagcacgg cgcccgtact
     8521 gagcgtggta aagacgctga gaagcagcag gcaaaacgcc acgccggctc cttcccatag
     8581 cgaaggcggg tgaaccggaa gggtggcaag aggcacgggt cggcgctctg gcggccgggg
     8641 aaactggatg gcgggctgac ccatgcgctg cggtcctggg gcgaaacgaa ccagacgccc
     8701 ctagtgtatc cagaccgtgg cgtgacgaca gtacctattt tgtagaggcg ctattggccg
     8761 tcgagaaggt gcagttcttt ccccggctta aagcgaactg ccttgcccgg ggcgatgctg
     8821 acttcggctc cggtgcgcgg attccggcca attccggttt tgcggggacg gacgttgaaa
     8881 acaccgaagc cgcggagttc aatccgctga ccctctgcaa gagcccgctt caggctctcg
     8941 aagaccgtat cgacagcagc ctcggccttg ttgcgctgca ggccggtgcg ttcgatcacc
     9001 tggtgaatga tgtcctgctt tatcaaggtt ttcccccgtc aggcgaagtg tccgtctcaa
     9061 cgctgatcgt agaaacgttt gatggcattg tcaatgtggc ggccgtcccg taagatgcaa
     9121 tttttacccc tatttgcggg ccgcaaggtc ccagccggtg cgctcaggcc ggccaacgca
     9181 ggattggaac cggaaaaagg ccgttctgga ccagaacggc agggagggac atggccatca
     9241 agagtgatcg ctggatccgg gaacaggccc tgaagggcat gatcgaccca ttcagcgaaa
     9301 agcaggtgcg ggagggcgtc gtctcctacg ggctctcttc ctacggatac gatctgcgcg
     9361 tttccgacga atttcggatc ttcaccaatg tcaacagcgc cgtcgtcgac cccaagaagt
     9421 tcgacgagcg ctccttcgta accgtacagt cggaatccat cctggtaccc ccgaacagct
     9481 tcgccctggc acgatcggtc gagtattttc ggattccgcg tgacgttctc acgatttgcg
     9541 tgggaaaatc cacatacgcc cgttgcggaa tcatcgtcaa tgtcacgccc ttcgaaccag
     9601 agtgggaagg cttcgtgaca cttgagattt ccaacacgac gccgctgccg gcgcgcgttt
     9661 atgcgaacga aggattgtgt cagattctgt ttttccagtc ggacgagccc tgtgaaatca
     9721 gctacgccga ccggaaaggc aagtatcaaa aacagcaggg aattgttctc ccaaaattgt
     9781 aggagctatg gaaacaggca gtcacaccac tcttaaggca aaagttggac tactggattt
     9841 cgagccgctt cgaatcgcgg ggttgcggga gatctttgcc gagcaaccgg attttcaggt
     9901 catcgcaacc gactgggcga gcgccttcag ggattcggct ctcgatctgg tcgtcgtcgt
     9961 cttgcgggac caggcctttt ccctggcctt gctggccagg ctgcgaaccc gccatcccgg
    10021 attgaaggtt ctggtcatcg gtacgggatc cgagcctgag aagatccttg aggccatcgg
    10081 agcgggcgcg aaaggatggc tggaggagac cgccagcccg gaacaagtca tgcatgccgc
    10141 atgtgccatt ttggatggct ccatctgggc gcctcggcag gtcctggcga ccttcgtcga
    10201 ccgcgctctg gaaggatctg cggcacggcg tcgccggtcg agtccgcgct tcaccgagcg
    10261 cgaagaggaa gtcctgcagc agttggtgct ggcgcgctct aatcgtgaaa tcgcccaggc
    10321 actgagcatc cgcgagcaga ccgtgaagtc ctatgtagca cggctgatgc gtaaggttgg
    10381 agtggggaat cgtattgcgc tgtcggtgca ggctgccagc ggagcctggg gcgaaaagaa
    10441 gctctagagc gctccccggg gcttagttaa cagaatatct gttatc
//
LOCUS       DASZYN010000204        15066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_390447, whole genome shotgun sequence.
ACCESSION   DASZYN010000204 DASZYN010000000
VERSION     DASZYN010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 15066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15066
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390447"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VHE33_05260"
     CDS             <1..481
                     /locus_tag="VHE33_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HVT96895.1"
                     /translation="DERSAPLGKVIADLFEFLAEPHLIQPTIIYEFPTAVSPLSKQKP
                     DEPDWVERFEFYIGGFELGNAFSELNDPEEQFRRFKDQLEQHERGDEEAHQMDLDYVR
                     ALAYGLPPTAGEGIGIDRLTMILTGARSIRDVILFPLLRPQAKEPEAGGGKQEPASS"
     gene            complement(504..2009)
                     /locus_tag="VHE33_05265"
     CDS             complement(504..2009)
                     /locus_tag="VHE33_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HVT96896.1"
                     /translation="MHSHYDPCMLDVSAGIVPVIPIDREADAPLHRQVYAGFRDAILR
                     GDLAAGQQVPSSRSLAAELEISRFPVLDAYAQLLAEGYFESRVGAGTFVASSLPYRAS
                     NPKRPAEEAPSSRRTSRRSALFPAYDPPAWRSGWGSFAVHQPALDHFPFELWSKLVAR
                     HSRTLRIHAPGNIHPLGLQPFREAICSYLRTARAVRCEPGQIMVVSGSQQALDIVTRV
                     LLDPGDAAWMEEPCYPLMRSLLTGSGCRAIPVPVDEDGLDVTAGMRLAPDARVAFVTP
                     SHHYALGVTMSASRRFQLLEWSRQASSWIVEDDYDSEYRFDTMPIPSLQGLDPCDRVI
                     YIGTFSKVLRPSLRLGYIVIPADLVDRFAAVRFSMDIFPSYLFQEALTDFMQDGHFAR
                     HIRRMRALYKGRRAALVECLRAEFGDFLKIHGSEAGMHLTVTFPQAFRAGAVSDVDIA
                     TRAAKDKLWLWPLSPCYTNGRARQGFILGFGNTPEEQMPGAVRKLRQLLSA"
     gene            complement(2105..2908)
                     /locus_tag="VHE33_05270"
     CDS             complement(2105..2908)
                     /locus_tag="VHE33_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HVT96897.1"
                     /translation="MPHYATLHTHTDSGIHTILLNRPEKRNALSHQLIEELTAALESF
                     AANPACRVLIVTGAGSAFCAGLDLEHLRAMAEHSGASATLDYRLDAERIAGLFRTLYT
                     LPKPSIAAVNGPAIAGGMGIATLCDFTLAVPEAKFGYTEVRIGFIPAIVSAFLRTQIG
                     EKACRDLLLTGRLLSAEEALALGLVTRIVPEPDLIPESTALAHRLLRNSPAAMESTKR
                     LLLRFTDRHLPQDIEAAIQASVDARSSADFQEGVSSFLEKRDPLWPSTR"
     gene            complement(3009..3311)
                     /locus_tag="VHE33_05275"
     CDS             complement(3009..3311)
                     /locus_tag="VHE33_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96898.1"
                     /translation="MKKNLLIVATAALLSVPCFSSASAQRIYVRIGPPPVVVEHPGPR
                     PHAGWVWVRGYHRWDGARYVWVPGHWIEPPRRHAAWVDGHWRHGRGGWYWVDGHWR"
     gene            complement(3484..4077)
                     /locus_tag="VHE33_05280"
     CDS             complement(3484..4077)
                     /locus_tag="VHE33_05280"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HVT96899.1"
                     /translation="MLPARDSRLVQIVDAAVADAARRSGAWLKCGPGCTQCCIGVFPI
                     SQLDAARLREGLAALDSTDAPRAAAVRQRARQSIARITEGFPGDVETGILDDEAADLL
                     EDFANDEPCPVLDPATGLCDLYAARPMTCRVFGPPLRVEGGIGVCELCYRSATEEEIA
                     ACELHLDTEDLESSLEAEAEQSIARSGNTIVAFALMD"
     gene            4128..4877
                     /locus_tag="VHE33_05285"
     CDS             4128..4877
                     /locus_tag="VHE33_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HVT96900.1"
                     /translation="MTEEKIVVGPEDRVFVLTGAGVSAESGLKTFRDQGGLWEGHRVE
                     DVATPEGWAADAEQVWRFYSERRKGALQAQPNPAHRALAEMEKRLGDRFFLVTQNVDD
                     LHERAGSTRLVHMHGELFMSRCENSSAGGMGGFGCARPPFRDDALYETRAAIARCECG
                     GRIRPHIVWFGEIPLEMDRIQREIDRATVMLVVGTSGAVYPAANFVRWAGPRVRKYYI
                     GPEEPMNTPAFTRLVPGNAGEVLPGLTEIVD"
     gene            complement(4885..5301)
                     /locus_tag="VHE33_05290"
     CDS             complement(4885..5301)
                     /locus_tag="VHE33_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HVT96901.1"
                     /translation="MSLEFSELAAELDRVASNVALRKGTALFHSGDPLSGVFVVRKGA
                     VAMSVDGQNAIYPPRTLGPGEIAGLPATLTGHYSLTAKIEQDSELGFVPGPKVTEILE
                     CNPRLCMIAMRLMSDEIARVRSALKETPPLAHHDEP"
     gene            5745..6344
                     /locus_tag="VHE33_05295"
     CDS             5745..6344
                     /locus_tag="VHE33_05295"
                     /inference="COORDINATES: protein motif:HMM:NF025268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HVT96902.1"
                     /translation="MILSWLRRRSSSRALLLAYVSIVLPLAARAAPPDPGPSAAEILH
                     RVEERAKAEHKNILLDFDASWCGNCKLYDRFREDPQMRPLLSRAFVFATMDTGERSSD
                     TQHADTPGGAAFEDSIGGKDAGWPFLVMLDANGKPLVDSNRPDRTSRSGRSNVGYPAA
                     PEEVDWFIEMLRRGAPSLSQQDLASVHAWLTAHSPMQHH"
     gene            complement(6356..7540)
                     /locus_tag="VHE33_05300"
     CDS             complement(6356..7540)
                     /locus_tag="VHE33_05300"
                     /inference="COORDINATES: protein motif:HMM:NF005761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain"
                     /protein_id="HVT96903.1"
                     /translation="MRILISGAGVAGPTLAWWLLRHGFTPTLVESSPHLRTGGYVIDF
                     WGAGFEIANRMGMLPSIRPHGYIVREIRMVDDRGRTAVSLPTRAFFKMTQGQFLSLPR
                     GDLAGCIYAALDGKVETLFGDRVVRLEQSESGVDVGFASRPARHFDLVVGCDGLHSGI
                     RQLVYGDEAQFEKYLGFKVAAFQTQGYGPRDELVYMMHSAVGRQLSRFSMRDDRTMFL
                     FTFRDEDRSLPSTLAAQKALLRERFGGMGWEAPQILDALDRASDLYIDRVSQIHMGPA
                     PTSWSRGRVTLLGDAAFAVSLLAGQGTALAMIAAYILAGELRRANGDYQHAFAQYQAR
                     FGPFVLGKQKAAPRLANFFAPRSQFAIFVHHQVMNRMRIPWVADLAIRRDIADRIELP
                     DY"
     gene            7638..8123
                     /locus_tag="VHE33_05305"
     CDS             7638..8123
                     /locus_tag="VHE33_05305"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVT96904.1"
                     /translation="MRSKTQRAASIQPAMMGGNDKQMMRELAWFRYTLRRFLRFSEKA
                     ARACGVTPQQHQLMLGIAGFTEEGRATISELAEFLQERNNSVVGLVERAADSGLVRRS
                     ASKTDRRQVVVTLTQRGEEILSTLTYLHHDEARRLRAGFLRAGLRDEMKTGRAAARKS
                     A"
     gene            8123..9910
                     /locus_tag="VHE33_05310"
     CDS             8123..9910
                     /locus_tag="VHE33_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HVT96905.1"
                     /translation="MPKSANSRRRGWLVRHPLLRRYFPLVHEDLVKVYARDLRKWLIV
                     APIIGAVTGLAITAIAIVILHKMWPPVLRYYLAHHWAIIPGLVLGFVATGLIMQWFTP
                     DPDEHSTEEIIRSYHEHAGDIDVRPFFAKLLAAVTTVGFGGSAALEGPSIYGGGAIGS
                     WLWAKMRGLGLDARDRRIMLITGAAAGMSAVFRAPLTGIVFALEMPYRDDLAHEALVP
                     SLIASVVSYVTLTSFLGAEPLFNFIHAEHFTRHDLYWSALLGVIVGLVAMAFVVTFRR
                     ARSFFVDWGIPHWVKMGVGGLLTGVCGVVFLHFYNGPLVPIGANYEAAEMILAGHHGT
                     QQLLAFSGLKLAATIFSLGAGGVSAMFVPLFLTGGALGTAFAQTIVHSPDAGLYAAVG
                     MASFISAGYKTPLAAAVFVAEATGAHAFIIPALIGAAVAYAVSGDASASGDQRLHEGV
                     KLQELTETPVSEVMQQQLVAAQGAMTVTEFVRTLSPHSHHDTFPVFDGHQLLGAVTLW
                     RVLHLPPDRWKTTHVRELIDARIGRVTPECDVTEALRLLLGEHAQPMLLVVSDEGKMQ
                     GIVTRTDILHALKTRKAETAGDFDEPLVV"
     gene            9956..11788
                     /locus_tag="VHE33_05315"
     CDS             9956..11788
                     /locus_tag="VHE33_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HVT96906.1"
                     /translation="MAEFPIPEAATNPEFQSSRKGQFGVALEPEVIKICLFALGVGLV
                     AGLVAQGLLELIYLFTNIFFYGHFSFAITNPAGNHLGLWEILIPPLGGLLVGIMIYFW
                     EPTLKGHGIPEAMESVLFGHSRMRIRVAILKPLATAFAIGTGGPFGAEGPIIQTGAAL
                     GSLFGQAVGLTPYYRRVLLASGAAAGMAATFTAPLAGILVAVELLLFELRARSFIPVA
                     LAAAVATGVRIHFAGWKPLFPTPAFRMTGFRELWLFALLGLLMGVVGWVMIRVLGWLE
                     DLFDHLPLKAAAIWAPVIGALILGGLGYFYPQVLGTSYDTIRDMLNDRETARHLIGIS
                     VAKFWALTISLGSGTTGGVFAPSLVVGGGLGAAFAMGMQHLLPTVVSDPAFYALVAMA
                     AVFGGIARAPFTSIVFLFELSHNPNALLPLIVAVMVSDGFVRLCSRDSIMTIKLVKRG
                     LIVLQDYSVPVLMRAKIDQVMRKDFTQFSAEDELRSVLEKYVPNDHALMPIIDAAGAL
                     IGIVEPHDLLRTEPPDHHFKMRELARRDYVLAYPGELVDQVHRDMMLKNTENVVVVEA
                     QGPRKPIGIARANDILQLRRWLMEEETREPRKIKERQQAITNNQ"
     gene            11821..12234
                     /locus_tag="VHE33_05320"
     CDS             11821..12234
                     /locus_tag="VHE33_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT96907.1"
                     /translation="MAIDVRGVCPLLQVYDMPTSVKFYRDKLGFEVAMTSPVMGPDKF
                     HWALLRLGSTEIMLNTAYEFDEQRPVPADRARVAAHRDTCLYFPCPDVEGAYEELHDK
                     GVAVKPPRIAPYGMKQLSLADPDGYELCFQWKAEE"
     gene            12415..13158
                     /locus_tag="VHE33_05325"
     CDS             12415..13158
                     /locus_tag="VHE33_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96908.1"
                     /translation="MATSAQSSPVVSSAKRVPGTAGRYFYFFMSLLIAATVIYGFSHT
                     VNQNLLHANPIRPWLLWVHGTLFSAWVGFFILQSALVRTRNVALHRVLGWFGVALAAA
                     MTVVGLWTSIVMGRFDVAVLNEKNQATFLLVPFWDIACFAACFGLAVLWRKKPELHRR
                     LMLIATCALTAAAWGRMPVPPAVGLFFYVGVDALIVLGVVRDLVVTRRVHKVYLWALP
                     VFAVGQFLVMRVILTAPAWWMKIAHALVG"
     gene            13224..14525
                     /gene="hmgA"
                     /locus_tag="VHE33_05330"
     CDS             13224..14525
                     /gene="hmgA"
                     /locus_tag="VHE33_05330"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HVT96909.1"
                     /translation="MAELKYQSGFGNEFATEAVKGALPEGQNAPQKSALGLYTEQLSG
                     TAFTAPRATNRRTWTYRIRPSVTHKPFSEIPGGLGLFHSGPFTEVPSPPNQMRWNPIP
                     MPEKKTDFVDGIVTMGGSGDPAMQIGVGVHLYAANAPMQGRYFYNADGEMMIVPQQGR
                     LGIHTELGILDVKPGEIGVIPRGIKIRVVMEGPSRGYICENYGLPFRLPELGAIGANG
                     LANPRDFLAPVAAYEDEDGDFRIVGKFLGRMWEAEIDHSPLDVVAWHGNYAPYKYDLA
                     RFNCINTVSFDHPDPSIYTVMTSPSLMPGTANVDFAIFPPRWVVAEHTFRPPWFHRNM
                     MNEFMGLVFGEYDAKAEGFLPGGASLHNCMSGHGPDAETWEKASNAELKPVKLDNTLA
                     FMWETGLPVRPTKFALETKILQHEYFECWQGLKKHFKGPGK"
     gene            14522..>15066
                     /locus_tag="VHE33_05335"
     CDS             14522..>15066
                     /locus_tag="VHE33_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96910.1"
                     /translation="MSAVQALLGRILEYAGLFPPAALDMESALRNYQRYTETDEFWLL
                     GGFVLPASRLVEFAGAFERVWGTEQERPWTLKVVCAGETADDARAIAEFQQGAVFIGS
                     LEARAANGRVAKEVLERLPAARSRYVEFPPEQAAEVLPVLADHGARAKIRMGGAGVPI
                     PSAEAVARFLLACAKERVAWKA"
BASE COUNT         2855 a         4341 c         5035 g         2835 t
ORIGIN      
        1 cgatgagcgg agcgcaccgc tgggcaaggt cattgcagac ctgttcgagt ttctcgccga
       61 gccgcacctg atccagccga cgatcatcta cgaattcccg acagccgtct cccccctctc
      121 caagcaaaag cccgacgagc ccgactgggt cgagcgcttt gagttctaca tcggcggatt
      181 cgagctcggc aacgccttct ctgagctgaa cgaccccgag gagcagttcc gccgcttcaa
      241 agaccagctt gaacagcacg agcgcggcga cgaggaagcc caccagatgg acctcgacta
      301 cgtccgcgcc ctggcctacg ggctgcctcc cacagcgggc gagggcatcg gcatcgatcg
      361 gctgacgatg atcctcaccg gtgcgcgctc catccgcgac gtgatcctgt tcccgctgct
      421 ccggccgcag gcgaaggagc cggaagccgg cggcgggaag caggaaccag caagcagcta
      481 ggtctggcgg ggacccgaag aagttacgcc gacagcagct gccgaagctt ccggactgcc
      541 ccaggcatct gctcttcggg cgtattgccg aagccaagga tgaatccctg gcgtgctcgt
      601 ccgttcgtgt agcagggaga gagcggccag agccacagct tgtccttcgc ggcgcgcgta
      661 gcgatgtcca catcggagac ggcgccggct cgaaaggcct gcgggaaggt gacggtgagg
      721 tgcatgccag cttccgagcc gtgaatcttc agaaaatcgc cgaattcagc gcgcagacat
      781 tcgaccagtg cggcgcggcg acccttgtag agagcgcgca tgcggcgaat gtgtcgcgca
      841 aagtgtccgt cctgcatgaa atccgtgagg gcctcctgaa agagatacga ggggaagatg
      901 tccatggaga agcggacggc ggcgaagcgg tcgacaaggt cggcggggat gacgatgtag
      961 ccaagacgca gcgaagggcg cagtaccttg ctgaatgttc cgatgtagat gacgcggtcg
     1021 cacgggtcaa ggccctggag cgatggaatc ggcatcgtgt cgaaccggta ctcgctgtca
     1081 tagtcgtctt cgacgatcca ggatgacgcc tggcgggacc actcaaggag ctggaaacgc
     1141 cgtgacgcgc tcatcgtcac gcccagggcg taatggtgcg agggcgtgac gaaggcgaca
     1201 cgagcatcag gagccagacg catgccggct gttacatcca gtccatcctc atcaactgga
     1261 accggaatcg cgcggcagcc ggaacccgtc agtagagaac gcatgagcgg gtagcagggc
     1321 tcttccatcc aggccgcatc gccggggtcg agcagaacac gggtcacgat atcgagggcc
     1381 tgctgcgagc cggagacgac catgatctgg cccggttcac agcgaactgc acgcgccgtg
     1441 cgcaggtacg agcagatggc ttcgcgaaac ggctgcagtc cgagggggtg aatgttgcct
     1501 ggtgcgtgga tgcgcagggt gcggctgtgc cgggccacca gttttgacca aagttcgaaa
     1561 gggaagtgat cgagcgccgg ctgatgcacg gcgaaggatc cccacccgct ccgccacgca
     1621 ggaggatcgt aggcgggaaa cagggcagag cggcgcgagg tgcgtcggga ggagggcgct
     1681 tcctcggctg gacgtttcgg gttcgaagcg cggtagggca gcgaagatgc tacgaaggtg
     1741 ccggcgccga cccggctttc gaaatagcct tcggccagca gctgggcgta ggcgtcgagc
     1801 acgggaaagc gagagatttc gagttctgct gcaagcgaac ggctggaggg aacctgctgc
     1861 ccggcggcca gatcgccgcg gagtatcgcg tcgcggaacc cggcgtagac ctgccggtgg
     1921 agtggcgcgt cggcttcgcg gtcgatgggg atgacgggaa caatgccagc ggaaacgtcg
     1981 agcatgcagg gatcgtagtg gctatgcata aaatcctgca agttgtcatg tgaagggacc
     2041 acttcaatcc aaaaagtggc tatggcaccg ccgtccggtt gtatacctgc agcgatcagc
     2101 cgatttagcg cgtcgagggc cagagcggat cacgcttttc cagaaaagag ctgacgcctt
     2161 cctggaaatc agcactggag cgcgcatcga cactggcctg aatcgccgcc tcgatgtcct
     2221 gaggcaggtg gcggtcggta aaccggagca gcagccgttt cgtggattcc atcgccgccg
     2281 ggctgttgcg gagcaaacga tgcgcgagag ctgtcgattc cggaatcagg tcgggctcag
     2341 gcacgatccg cgtgacgagc ccaagcgcaa gcgcctcctc cgcggacagg aggcgaccgg
     2401 tgagaaggag gtcgcggcac gccttttcgc cgatctgggt gcgaaggaaa gccgacacga
     2461 tggctgggat gaatccgatg cggacctcgg tgtatccgaa tttggcttcg ggcacagcga
     2521 gcgtgaagtc gcacagggtc gcgataccca tgcctccggc gatagccggt ccgttgaccg
     2581 ctgcgatgct tggcttaggc agcgtgtata gggtccgaaa cagtccggcg atcctttccg
     2641 cgtcgaggcg gtaatcgagg gtcgctgaag ctccggagtg ctcagccatg gcacggaggt
     2701 gctcgagatc gagcccggcg cagaacgcgg atccggcgcc cgtgacgatc aggacgcggc
     2761 aggccgggtt ggcagcgaag gactcgagcg cggccgtgag ctcttcgatc agttggtgac
     2821 tcagcgcgtt gcgcttctcc gggcggttca gaagaatggt gtgaatgccg gagtcggtgt
     2881 gcgtgtggag cgtggcgtag tgaggcatgg cgagtctctt cgagagggta cagcctgccg
     2941 gtagcacgca aaagaggaaa accgacccgt ccggtctgag acgagtcggt tttcagggac
     3001 ggccgatttt accgccagtg gccgtcaacc cagtaccagc caccgcgtcc gtggcgccag
     3061 tgcccatcga cccacgcggc gtgccggcgt ggcggctcga tccagtggcc aggaacccag
     3121 acgtagcgag cgccgtccca gcggtgataa ccgcgaaccc aaacccaacc cgcatggggc
     3181 cgtgggccag gatgttcgac gacgaccggc ggaggaccga tgcgaacgta aatccgttgc
     3241 gcagacgcgg atgagaagca gggaacgctg agaagtgcag ccgttgcaac gatcagcagg
     3301 ttcttcttca tagagtgggc tttttccttt caggggatgg agttggtacc gcctgcggct
     3361 ctggtttcaa aacttgccgc acacgcgatg aaacccgacc ggcgagcctg ggttgcgcag
     3421 aaaatcttct cacgaaatac gagcattttg gctcgcagcg gatcgaagga ggagccgggt
     3481 tcatcaatcc atcagcgcaa aagccacgat cgtattgccg ctgcgagcaa tcgactgctc
     3541 ggcctctgcc tcgagagaag actccaggtc ctcagtatcg agatggagtt cgcaagccgc
     3601 gatctcttcc tccgtcgcac tgcggtaaca gagttcgcaa acgccaatgc cgccttcgac
     3661 gcgcagcggc ggaccaaaga cccggcaggt catcgggcga gcagcgtaga gatcgcaaag
     3721 gccggttgcg gggtcgagta cggggcaggg ctcgtcgtta gcaaaatctt cgagaaggtc
     3781 cgcagcttca tcgtcgagga ttccggtttc cacgtcgccc gggaacccct cggtgatcct
     3841 cgcgatcgac tggcgggcgc gttgccggac ggcggcagcg cgcggcgcgt ccgtggaatc
     3901 gagcgcagcg aggccttccc gcagtcgtgc cgcatcgagt tgagaaatgg ggaacacgcc
     3961 gatacagcac tgcgtgcatc ccggaccgca cttaagccag gcgcctgagc gtcgggccgc
     4021 gtccgcaacg gcggcatcga caatctgaac caaccgggag tcgcgcgcgg ggagcatggg
     4081 atcagaatag tggaggcagg tgcgagcatg gatcggcgag gtggtaaatg accgaagaga
     4141 agatagttgt cggcccggag gatcgcgtct ttgtgttgac cggcgccggc gtgagcgcag
     4201 agagcgggct gaagaccttc cgcgatcagg gcgggctgtg ggaagggcat cgggttgaag
     4261 atgtcgcaac ccctgagggt tgggctgccg atgccgagca ggtgtggcgc ttctacagcg
     4321 agaggcgcaa aggcgccctc caggcacaac cgaatcctgc gcatcgcgcg ctggcggaaa
     4381 tggaaaagag acttggggat cgattctttc tggtcacgca gaacgtcgac gatctgcatg
     4441 agcgtgccgg ttccacgcgc ctggtgcaca tgcacggaga gctgttcatg tcgaggtgcg
     4501 aaaacagcag cgccggcggc atgggcggtt ttggatgcgc gcggccgccc ttccgggacg
     4561 atgcgcttta cgagacgcgg gcggccattg cgcgctgcga atgtggcggc cgcatccggc
     4621 cccatatcgt atggttcggg gagattcctc tggagatgga ccgaatccag cgcgagatcg
     4681 accgggcgac ggtgatgctg gtggtgggaa cgtcgggagc tgtgtatccc gctgcgaact
     4741 ttgtgcgttg ggcggggccg cgcgtgcgca agtactacat cgggccggaa gagccgatga
     4801 atacgccggc ctttacgcgg ctggtgcctg gaaatgcggg ggaagtgctg ccggggctga
     4861 cggagatcgt cgactaagca gaacctaagg ctcgtcgtga tgcgcgagcg gtggcgtctc
     4921 cttcagggcg gagcgcaccc ttgcgatctc gtcgctcatc aggcgcatgg cgatcatgca
     4981 gagacgcgga ttgcactcga gtatttcggt gactttggga ccaggcacga agcccagctc
     5041 cgagtcctgc tcgatcttcg ccgtcaggct gtaatggccg gtgagggtgg caggaaggcc
     5101 ggcaatctcc ccagggccga gcgtacgcgg cgggtagatg gcgttctggc catcaacgga
     5161 catggcgaca gcgcctttgc gaacgacaaa gacgccggaa agcggatcac ccgagtgaaa
     5221 gagcgctgtg ccctttcgaa gggcgacgtt cgaggcgact cgatcaagct ccgcagctaa
     5281 ctctgagaat tctaaggaca taccggacaa cgggccgtgg agcgagtcag cggtggggcc
     5341 cctttccaga attctcttcg atttccggaa gaacgaatgt gatctgcgtc acatccggcc
     5401 aggaaatggc gcgtcatggc gttgcccgcc tcgcacctaa gtgcatacgg tcgttggacc
     5461 ccacaaaaag ctccttaaac gcagcctgcg tcttctccgc ggcgaaccgg agcggctcga
     5521 ccgactgcac gatcaacaac cgggttgccg aggctgtgga cgcttctgcc agggcacggc
     5581 cgatgcgcga actggcacgg gcgatcaaaa tcgcattgtc cgtggtgtgt gcgtggatga
     5641 tgctccttca cggtgagaac gcacgttcgg ttcgcgcgcg ggtgctctcg tcgcgcgaga
     5701 gtgcgacata atgaccggaa ggcattattc ttcgggagat ttacgtgatt ctgtcctggc
     5761 ttcggaggag aagttcctcg cgcgctctgc ttctggcgta cgtctcaatc gtcttgcccc
     5821 tggccgccag agcggctcca cccgacccgg gtccttcggc tgccgagatc ctgcatcgcg
     5881 tcgaagaacg ggcaaaggcc gagcacaaaa acatcctgct ggacttcgac gcgtcatggt
     5941 gtggcaactg taagctctac gaccgattcc gcgaagatcc ccaaatgcgc ccgcttttga
     6001 gccgggcatt tgtcttcgca accatggata ccggcgagcg gtccagcgac actcaacatg
     6061 ccgatacgcc tggcggcgca gcattcgagg attccattgg cggcaaggat gcgggttggc
     6121 ctttcctggt catgctcgat gccaacggca agccgcttgt ggattcaaac cggccggatc
     6181 ggacttcgag gagtggcaga agcaacgttg gctatccggc cgctcccgaa gaggtggact
     6241 ggttcatcga gatgctgcgc cgcggggcgc cgtcgttgag ccagcaggat ctcgccagcg
     6301 ttcacgcatg gctcaccgct cactcgccga tgcaacatca ttaaggagtc gcaactcagt
     6361 aatctggtaa ttcgatccga tcggcgatgt cgcggcgaat cgcgagatcg gcgacccagg
     6421 ggattctcat ccggttcatg acctggtggt gaacgaaaat tgcgaattgc gagcgtggag
     6481 cgaaaaaatt tgccagccgc ggcgccgcct tttgcttgcc cagcacaaag ggcccgaaac
     6541 gggcctgata ctgtgcgaag gcgtgctggt aatcgccatt ggcacgtcgc aattctccgg
     6601 ccagaatgta ggcggcgatc atcgccagcg cggttccctg acctgccaga agcgagacgg
     6661 caaacgcggc atcgccgagc aatgtgacac gaccgcgcga ccaggaagtg ggcgccgggc
     6721 ccatgtgaat ctggcttacg cgatcgatgt acagatctga agctcgatcc agcgcgtcca
     6781 gaatctgcgg cgcctcccaa cccatgccgc cgaatctttc gcgcagaagt gctttctgtg
     6841 ccgccagtgt cgatggcagc gatcgatcct cgtctctgaa cgtgaacaaa aacatcgtgc
     6901 ggtcgtcgcg catcgagaag cgtgaaagtt ggcggcccac ggccgaatgc atcatgtaaa
     6961 cgagttcgtc gcgaggtccg taaccctgtg tttggaaagc tgcgaccttg aatccaagat
     7021 atttttcaaa ctgcgcttcg tcgccgtaaa ccagctgccg aatgccggag tgcaggccgt
     7081 cgcagccaac caccagatcg aaatggcgag ccggacgtga tgcaaaaccc acatcaacac
     7141 cggattccga ctgctcaaga cgcacgacgc gatcgccgaa cagagtctcc actttgccat
     7201 ccagtgccgc gtagatgcag ccagccagat cgccccgcgg gaggctcagg aactggccct
     7261 gggtcatctt gaagaaagct cgcgtgggga gactgacggc ggttcggccg cgatcgtcga
     7321 ccatccgaat ctcgcgcacg atatagccgt gcgggcgaat gctgggcagc atgcccatgc
     7381 gattggcgat ctcaaagccg gcgccccaga agtcgatcac gtagccgccg gtgcgcaggt
     7441 gcggcgagga ttccaccagg gtcggcgtga atccgtggcg gagcagccac caggcgaggg
     7501 tcggtccggc aacgccggcg ccggaaatga ggatgcgcat tgcctacagg gtagcgcgcg
     7561 cacctcccca atgcggtctt tccggatagc agcccaacat ttgagaagga tcgcagtacg
     7621 aggtaaaata gatcgcagtg cgatctaaaa cacaaagagc agcgagcatc caaccggcaa
     7681 tgatgggcgg caacgacaag cagatgatgc gggagctggc gtggtttcgc tacacgctgc
     7741 gacggttcct ccgcttcagt gaaaaggccg cccgggcgtg cggagtcacg ccccagcagc
     7801 atcagttgat gctggggatc gcgggattca cggaagaagg gcgagccacc atttcagagc
     7861 tggccgagtt tctgcaggag cggaataact ccgtcgtcgg cctggtggag cgggccgcgg
     7921 acagcggact ggtgcggcgc agcgcgagca aaaccgatcg ccggcaagtc gtcgtgaccc
     7981 tcacccagcg cggcgaggaa attctgtcga cgctgaccta tctccatcac gacgaagcac
     8041 ggcggctgcg agctggattt ttgcgagccg gattgcggga tgagatgaaa acaggtcgcg
     8101 cggcggccag gaagtctgcg tgatgccaaa atctgcaaat agccggcgaa gaggctggct
     8161 ggttcggcat ccgctgctgc gtcgctattt tccgctggtg cacgaagacc tggtgaaggt
     8221 gtacgcgcgg gatctgcgca agtggctgat cgtcgcaccg atcatcggag ccgtcaccgg
     8281 cctggcgatc acagcgatcg cgatcgtgat cctgcacaag atgtggccgc cggtgctgcg
     8341 ctattacctt gcgcatcact gggccatcat tcccggattg gtgctgggct tcgtggcgac
     8401 cggcctgatc atgcagtggt tcacccccga tccggacgag cattccacgg aagagatcat
     8461 ccggtcctat cacgaacacg cgggcgacat cgacgttcgg ccctttttcg caaaactgct
     8521 tgcggcagtg accacagtcg gctttggcgg cagtgccgcg ctggagggcc caagcatcta
     8581 cgggggcggc gcgatcggct catggttgtg ggcaaagatg cgcggactgg gactggacgc
     8641 ccgcgatcgc cgcatcatgc tcattacggg agcggcagcg ggaatgtccg ccgtcttccg
     8701 tgcgccgctc accggcatcg tgtttgcgct ggaaatgccg taccgcgacg atctggcgca
     8761 cgaggcgctg gtgccatcgc tgatcgcgtc ggtggtttcc tacgtgacgc tcacgtcgtt
     8821 cctcggtgcg gagccgcttt tcaatttcat ccacgccgaa cacttcaccc ggcatgacct
     8881 gtactggagc gcgctgctgg gcgtgatcgt gggccttgtc gcaatggcct ttgtagtcac
     8941 attccggcgt gcgcgcagtt tcttcgtcga ttggggtatt ccgcactggg tgaagatggg
     9001 cgtcggcggc ctgcttacag gtgtttgcgg cgtcgtcttt ctgcatttct acaacggccc
     9061 gctcgtgccg attggggcaa actatgaggc cgccgagatg attctggcgg gccatcatgg
     9121 aacccaacag ttgctggcgt tcagcggact caagctcgcg gcgaccatct tttcgctggg
     9181 tgccggtggc gtgagcgcga tgttcgttcc gctgttcctg accggaggag cccttggcac
     9241 ggccttcgct cagaccatcg tccacagtcc cgatgccgga ctttacgcag cggttgggat
     9301 ggcgtccttc atctccgcgg gatacaagac tccgctggcg gcggccgtgt ttgtcgccga
     9361 agcgaccggt gctcatgcat tcattattcc ggcactgatt ggtgcagccg tagcctatgc
     9421 cgtttcaggc gatgcttcgg cgtcgggcga tcaacgactc catgaaggtg tgaagctcca
     9481 ggaactgact gaaactccgg tcagcgaagt gatgcagcag caactcgtcg cggcgcaggg
     9541 cgctatgacg gtgactgagt ttgtgcgcac cctgtcgcca cattcgcacc acgatacttt
     9601 cccggtcttc gacggacacc agttgctcgg cgcggtcacg ctctggaggg tcctgcacct
     9661 gcctcccgat cgctggaaaa cgacccatgt gcgggaatta atcgatgcgc gcatcggtcg
     9721 cgtgacgccg gagtgcgatg tgacggaggc tttgcggttg ctgttgggcg agcatgcgca
     9781 gcccatgctg ctggtggttt ccgacgaggg caaaatgcag ggcatcgtaa ccaggaccga
     9841 cattctgcat gcgctgaaga cgagaaaggc cgagactgcc ggagatttcg acgagccgct
     9901 ggtggtgtag gtccggagcc gcggactaag gcagacaacg aacgggagga tcgcgatggc
     9961 ggagtttccg attccagaag ccgcaaccaa tcctgaattt cagtcgagca ggaagggaca
    10021 gttcggcgtt gctctggagc cggaggtcat caagatatgc ctcttcgcac tgggtgtggg
    10081 actcgtcgcc ggcctggtgg cgcagggtct gctcgaactc atctacctgt tcacgaacat
    10141 cttcttctac ggtcactttt ccttcgccat cacgaatccg gccgggaacc acctgggcct
    10201 ctgggaaatt ctgatcccac cgctgggcgg cctgctggtc ggcatcatga tctatttctg
    10261 ggagccgacg ctgaagggcc acggcatccc ggaagcgatg gagtcggtgc tcttcggcca
    10321 cagccgcatg cggatccgtg tcgcaatcct caagcccctg gccacggcgt tcgctatcgg
    10381 gaccggcggc ccgtttggag cggaaggtcc gatcatccag accggcgctg cactcggctc
    10441 gctattcggc caggcagtcg gtctgacccc ctactaccgg cgcgtgctgc tggcatcggg
    10501 cgccgcagcg ggcatggccg cgaccttcac cgcgccactg gcagggattc tggtcgcagt
    10561 ggagctgctg ctgtttgaac tccgcgcgcg ctccttcatc ccggtggcgc ttgctgctgc
    10621 ggtcgctacc ggtgttcgca tccactttgc cggatggaag ccgctgtttc ccaccccggc
    10681 cttcaggatg acgggattca gggaactgtg gctgtttgcg cttctcggtt tgctcatggg
    10741 cgtggtggga tgggtcatga tccgcgttct cggctggctc gaggatctgt tcgaccatct
    10801 gcccctgaag gccgcagcta tctgggcgcc ggtcatcggc gcgctgatcc tcggtgggct
    10861 cggctacttc tacccccaag tgctcggcac cagttacgac accatccgcg acatgcttaa
    10921 cgaccgcgaa acagcccggc atctgatcgg catctcggtg gcaaagttct gggccctgac
    10981 gatctcgctg ggatcgggca ctaccggcgg cgtcttcgcg ccttctctgg tcgttggcgg
    11041 tggactgggc gcagcgttcg ccatggggat gcagcacctg ctgccgaccg tggtcagcga
    11101 tcctgcgttc tatgcgctgg tggccatggc ggcggtcttc ggtggcatcg ctcgcgcgcc
    11161 gttcaccagc attgtgtttc tattcgagct cagccacaat cccaatgctt tgctgccgct
    11221 gattgtggct gtgatggtgt cggacggttt cgtccgcctg tgcagccgcg attcgatcat
    11281 gaccatcaag ctggtcaagc gtgggctcat cgtgctgcag gattactctg tccccgttct
    11341 gatgcgggca aagatcgacc aggtcatgcg caaggacttc acgcagttct ccgccgagga
    11401 cgaattgcgc tccgtgctcg agaagtatgt gcccaatgac catgcgctca tgccgatcat
    11461 cgacgccgcg ggtgctctga ttggaattgt cgaaccgcac gatctcctcc gcaccgagcc
    11521 gccggatcac cacttcaaga tgcgcgagct ggcgcgtcgc gactacgtgc tggcctatcc
    11581 cggcgaactg gtggaccagg tgcatcgcga catgatgctc aagaacaccg aaaatgtcgt
    11641 cgtcgttgag gcccagggcc cacgcaagcc gatcggcatt gcgcgcgcca atgacatcct
    11701 ccagcttcgg cgctggctca tggaagaaga gacccgcgag ccgcgaaaga ttaaagagcg
    11761 gcaacaggca atcaccaaca accaatagcg ctatgctgat ggccatcggt gaggtgagcc
    11821 atggccatcg atgtgcgcgg agtgtgcccg ctgctgcagg tgtacgacat gccgacctcg
    11881 gtgaagttct atcgggacaa gctcgggttt gaggtggcga tgacgtcgcc tgtgatgggc
    11941 ccggataagt ttcactgggc actgctgcgg ctgggatcga cagagatcat gctgaacacg
    12001 gcctatgaat tcgacgagca gcggccggtg ccggcggatc gagcgcgcgt agccgcacat
    12061 cgcgatacgt gcctctattt tccctgtccc gatgtggaag gcgcctacga ggagttgcac
    12121 gacaagggcg tggcggttaa gccgccgcgc attgcgccct atgggatgaa gcaactctcg
    12181 ctcgccgatc ccgatggcta cgaactgtgc ttccagtgga aggcagaaga gtgaagaggc
    12241 aggggatagc caatagccaa cagggaatag ccaaaggctg attgggagtt ggcctgtcgg
    12301 gcggggcctt tttcacgcgg cggacgcgca acggcgagtt tgttgtggtc attaccgcac
    12361 gcgattgtta ccatagccca acttcagtcc ccaggctcct ccaggtgagg ttctatggct
    12421 acgtcagcac agagctctcc ggtcgtctcc agcgcgaaac gcgttccagg gaccgcgggg
    12481 cggtacttct acttcttcat gtcgctgctt attgcagcga ctgtgatcta cggcttcagc
    12541 catacggtca atcaaaacct gctccatgcg aatcccatca ggccctggct gctgtgggtg
    12601 cacggaactc tgttttcggc gtgggtcgga ttcttcattc tgcaatcggc gctggtgcgg
    12661 acgcggaacg tggcgctgca ccgggtgctg gggtggttcg gcgtggccct ggcggcggcg
    12721 atgacggtgg tggggctctg gacgtcgatc gtgatggggc gcttcgatgt cgccgtgctg
    12781 aacgagaaga atcaggcgac ctttctgctc gttccgttct gggatatcgc gtgctttgct
    12841 gcttgcttcg ggctcgcggt cctgtggcgc aagaagccgg agctgcaccg gcggctgatg
    12901 ctcatagcaa cgtgcgcact gacggcggcg gcgtgggggc ggatgccggt ccctccggcg
    12961 gtggggttgt ttttctatgt gggcgtcgat gcgctgattg tgctgggcgt cgtgcgggat
    13021 ctggttgtga cgcggcgcgt gcataaggtg tatctgtggg cgctgccggt tttcgccgtt
    13081 gggcagttcc tggtgatgcg cgtgatcctg acggcgccgg cgtggtggat gaagattgcg
    13141 cacgcgctgg tcggttgagg ttgccaagtc tccttccgtc ccggtagggt ggtaagtttt
    13201 tgtgacggga acgggaggcg aggatggcgg agctgaagta tcagtcaggg ttcggcaacg
    13261 agtttgcgac tgaagcggta aagggtgcgt tgcctgaagg gcagaatgcc ccgcaaaagt
    13321 cggcactggg actctatacg gaacagctga gcggaacagc gttcacggcg ccgcgggcga
    13381 ccaaccgccg cacgtggacc tatcgcattc gtccttcggt gacgcataag ccgttctcgg
    13441 aaataccggg tggtctgggt ctgtttcaca gcggaccatt caccgaggtt ccctcgcccc
    13501 ccaaccagat gcgctggaac ccgatcccca tgccggagaa gaagaccgac tttgtcgacg
    13561 gcatcgtgac gatgggaggc agtggcgatc ctgccatgca gatcggggtc ggcgttcatc
    13621 tctatgcggc gaacgcaccg atgcaggggc gctatttcta caacgccgat ggcgagatga
    13681 tgatcgtgcc ccagcagggt cggctgggaa tccataccga gttgggcatt ctggacgtga
    13741 agcccggcga gatcggcgtg atcccgcgcg gcatcaagat tcgcgttgtg atggaggggc
    13801 cgtcgcgcgg ctacatctgc gagaactatg ggctgccgtt ccggctgccg gaactgggtg
    13861 ccattggcgc gaatggactc gcgaatccgc gggatttcct tgcgccggtg gccgcctacg
    13921 aggatgagga cggagatttc cgcatcgtgg gcaagttcct cgggcgtatg tgggaggcgg
    13981 agatcgacca ctcgccgctc gatgtcgttg cgtggcacgg gaattacgcg ccgtataaat
    14041 acgacctggc gcgcttcaac tgcatcaaca cggtgagctt cgatcatccg gacccatcga
    14101 tctacaccgt gatgacgtcg ccgtcgctca tgccgggaac cgcaaacgtg gatttcgcca
    14161 tctttccacc gcggtgggtg gtggccgagc atacctttcg gccgccgtgg ttccatcgca
    14221 atatgatgaa tgagtttatg gggctggtgt tcggcgaata cgacgcgaag gcggaaggct
    14281 ttctgcctgg cggggcgagc ctgcacaatt gtatgagcgg gcacggaccc gatgcggaga
    14341 cctgggagaa ggccagcaac gcggaactga aaccggtgaa gctcgacaac accctggcgt
    14401 tcatgtggga gactggcctg ccggtgcggc cgacgaagtt tgcgctggag acgaagattt
    14461 tgcagcacga gtactttgaa tgctggcagg gactgaagaa gcacttcaag gggccaggca
    14521 aatgagcgca gtccaggcgt tgctgggcag aattctggag tatgcgggtc tgtttccgcc
    14581 ggcagcgctc gacatggaga gcgcgctgcg gaattaccag cgctatacgg agacggacga
    14641 attctggctc ctgggcggtt tcgtgctgcc ggccagtcgg ctggtggagt ttgcgggcgc
    14701 gtttgagcgc gtatggggta ccgaacaaga acggccgtgg acgctgaagg tggtgtgcgc
    14761 gggcgagacg gcggacgatg cgcgcgccat tgcggagttc cagcagggag ccgtattcat
    14821 tgggagcctc gaagccaggg cggcgaacgg gcgtgttgcg aaggaagtcc tggagcggct
    14881 tccggcggcg cggtcgcggt atgtcgagtt tccgccggag caggccgcgg aggtgctgcc
    14941 ggttctggcc gatcacggag cgcgggcgaa gattcgcatg ggtggggccg gcgtgccgat
    15001 accgagtgct gaagccgtgg cacggttttt gctcgcgtgt gcaaaggagc gcgtggcatg
    15061 gaaggc
//
LOCUS       DASZYN010000205         1910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_390487, whole genome shotgun sequence.
ACCESSION   DASZYN010000205 DASZYN010000000
VERSION     DASZYN010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390487"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VHE33_05340"
     CDS             <1..196
                     /locus_tag="VHE33_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96911.1"
                     /translation="ELEAPCSGIVYEILVREEETVTVGTVLAYIGKPDESFPVPNPPF
                     RMKPTPKQPAFLRRRRAKRR"
     gene            complement(347..934)
                     /locus_tag="VHE33_05345"
     CDS             complement(347..934)
                     /locus_tag="VHE33_05345"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVT96912.1"
                     /translation="MNKPAPANRPMRADAQRNYASLLSAARVAIAERGADIVLEDVAR
                     SAGVAIGTLYRHFPTRQDLLEAVFLDETNALRAHAEELASAPVPFDALMSWLRLQMDF
                     AAFGRSMGAAIMAAKQVPGTRIYEANKAMHEAGNGLLLRAQAAGHIRTDVYLVDVMRL
                     VYGIALVNEHASDPEGANRMLDLVIAGISTRPSRD"
     gene            1096..>1910
                     /locus_tag="VHE33_05350"
     CDS             1096..>1910
                     /locus_tag="VHE33_05350"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT96913.1"
                     /translation="MRVFVTGATGFIGVSVVRELMNAGHTVIGLARSDHGAALLKEAG
                     AEVHPGALDDLDSLHSGAATSDGVIHLAFQHGSADFAAALTTDLRAVETMGAALEGSG
                     KPFVTTAHLNGEASDNAVLRLSGVRSSIVTLSPSVHGEGDRHGFVPRLITIARTKGVS
                     AYIGDGSNRWPAVHRLDAAHLFRLALEAAPAGSRLYGAGDEGVPLRAIADLIGRHLNL
                     PVVSISREEADAHFGSFLSTVVATDISARLDMPRLSVQTRELLGWQPVHPRLLP"
BASE COUNT          373 a          566 c          568 g          403 t
ORIGIN      
        1 tgaactggag gctccttgca gtgggatcgt ctacgaaatt ctggtgagag aggaggaaac
       61 agtcactgtt gggaccgttc ttgcttacat cggaaaaccc gatgagtctt ttcctgtccc
      121 caatcctcct ttcagaatga agcctacgcc aaagcagcct gcatttctgc ggcggcggcg
      181 ggcaaagaga agatagaaaa tgagagcctg ttccaaggac tgacgagaaa gagtgttgcg
      241 agaagagtca ttacttcgat gtggatggga cgagagaagc agcgcgtcag cgggcatacc
      301 tcagagagca ggatcgactc ctcgcccaga ggtgatatac catctttcag tcccgactcg
      361 gcctggtgct gattccggcg atgacgagat cgagcatgcg gttggccccc tcaggatcgg
      421 acgcgtgttc attcaccagg gcgataccgt agacgagtcg catcacatcg accaggtaga
      481 catccgtgcg gatgtgtcct gcggcctggg cgcggagcaa caacccattt cccgcctcgt
      541 gcatggcttt gtttgcctca tagattctcg ttcccggaac ctgcttggca gccatgatgg
      601 ctgctcccat gctccgcccg aaagccgcga agtccatctg gagacgaagc cagctcatca
      661 aggcgtcgaa aggaaccgga gcgctggcga gttcttcagc gtgcgccctc aatgcattgg
      721 tctcatccag gaacaccgct tcaaggagat cctgacgcgt agggaagtgg cggtagagcg
      781 ttccgattgc gacccctgcc gacctggcaa cgtcctcaag aacgatgtca gcgccgcgct
      841 ctgcgatggc gactcgcgcc gcactcaaca ggctggcgta gttgcgttgg gcatcagctc
      901 gcatggggcg gttggctggt gctggcttgt tcatttcatg ctcctccttc ctcccagaaa
      961 tatgagccct tgacaaaatg aggaaccctc cgttaatata gagaggtaac gtgaggatcc
     1021 ctccgtttca tatggggagg tatcctcatt ttatcaccct ctgtttcatt ttaccaggag
     1081 aaggagaacg ttcttatgcg tgttttcgtc acaggagcaa ctggcttcat cggtgtctct
     1141 gtcgtccgcg aactcatgaa tgccggtcat acggttatcg gcttggcccg ctcagaccat
     1201 ggtgccgcgc tcctgaagga agctggagcg gaggtgcatc cgggtgccct tgacgacctc
     1261 gacagccttc acagcggtgc cgccacatcc gatggcgtca ttcacctggc gtttcagcac
     1321 ggcagcgcgg acttcgcagc cgcgctcaca accgatctgc gcgctgtcga gacgatgggg
     1381 gcggcgctcg aaggttccgg caagccgttc gtgactaccg ctcacctgaa cggggaggca
     1441 tcggataacg cggtgcttcg actgtctgga gtgcgctcat caatcgtcac gctcagtccg
     1501 tccgtgcatg gagagggcga caggcacggc tttgtgccga gactgatcac catcgctcgc
     1561 acgaagggcg tctctgccta catcggcgac gggtccaacc gctggcctgc cgtccaccgc
     1621 ctcgatgctg cgcacctgtt ccgcctggcc ctggaagccg ccccggcggg gtcacggctg
     1681 tacggggccg gcgatgaggg ggtgcccttg cgcgccattg ccgacctcat cggtcgccac
     1741 ctgaacctgc cagtggtcag catttcccgt gaagaggcag atgcccactt cggttctttt
     1801 ctcagcaccg tggtggcgac cgatatctca gcgaggctcg acatgcccag gttgagtgta
     1861 cagacacggg aactcctggg atggcagcct gtgcatccgc gacttctccc
//
LOCUS       DASZYN010000206         8196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_198791, whole genome shotgun sequence.
ACCESSION   DASZYN010000206 DASZYN010000000
VERSION     DASZYN010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8196
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198791"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3395)
                     /locus_tag="VHE33_05355"
     CDS             complement(<1..3395)
                     /locus_tag="VHE33_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT96914.1"
                     /translation="MPIGLGLAVAVLLMAQGAWAQANRGSITGTVADPSGAVVAGVAV
                     TATNTGTNIPTATVSNSEGIYVVPNLPPGVYSLEFRRDGFETLTHPDITVNSTEAVRI
                     DATLHVGAVTATVTVSGGAPVLDMESAAVGTNMKATIVNDLPLSIYGGGRFVEDFAVA
                     ITPGYSPISSPYGAVVNGGQWFTKDYTIDGTSGTSSVRGDSMDGGPSMEAVEELQAQT
                     SGLDAESSITGGGVMSFNLKSGTNKFHGSAFLYGHNELLDANTWTNDSLGLGKPKERA
                     WDTGGSIGGPIHKDKTFFFGTFERYQSIDFRLGSGGATVPLPAFLTGDFSSLMGPTLC
                     DFGGSTDLCSNTQAHKNTVGTPIMVTDNAGNQLQARENMIYDPDTGNQFTGNIIPPSR
                     ISPVAQKVNAYYANYKPQLGGLDNNARTLISNTPTQTPIWVVAKLDHVLRDQDRLSGS
                     WIYDHKPRTLDDGGGVWQEGTQSGGPLSNARVQMYRQQQWRLSEQHTFTPQMLNVLNF
                     GYNFDFNGSDPADPGNWNSQIGFADTGANNFPQISFSDNGSYGHSETFLGSTWQGNTK
                     GTYASVGDTLTWTKGRHNLSFGGVFDAHQINSHSGSGAYNFSFSYLNTAGPGYPYDGF
                     GFATYMLGQVNQGSESVPYNLYGRQKSLALFAQDNYKVTSRLTANLGLRWNYNGRFHE
                     KNGNWANFDPTVTSPVYGIPGALVFAKDGSDSFEKNEYYLNFGPTIGLAYQVHPKTVL
                     HASFGLIYNPVGVTFFTGVPNGFAPQLGSSAANNFSWDAPGGGGQYPGVFSKATLNTD
                     PSTMTPFPVSMDPRALRLGYSEALNAGIQQQLTPNTRLEISYVGNRGHRLTDAALAWN
                     ETSTDKFLALLKAHPDMNIWDYGQYIYSEADAAAYGVPYPYPGFWGPPLAALAPIPYM
                     AAAEVEYWYYPNLQYAGLPLGQSFYDSMVVDVVKRAGRGLTMDMNYVWSKTEGDSFSA
                     QQEYNGFYTPIQDFGKMSQAAHTITGYDLTHVVKGFASYQLPFGRGQQWLGNTSRVAN
                     GFIGGWTLAGTVDYYTGQPFSISAANPYWPLWGNIYPIFNVAGGKTSDPHKYVPIGDD
                     VTPPATNFYMPSTVASNPAPGVLPPRGATSALRCPGQANENVTV"
     gene            complement(3544..4827)
                     /locus_tag="VHE33_05360"
     CDS             complement(3544..4827)
                     /locus_tag="VHE33_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96915.1"
                     /translation="MRADIRGAYLLRKARGPSHMHDIGRRLAFSVALLLPAASMTADG
                     SGGPGSRVRMEVVRQCARPVLTIHSPGAGENKYGFEGGRVLKLGATYHLFTSEMTGEP
                     HWVKMKLAHWTSTDRLHWQRVDTLFESSGDFTGRDPRAALWSPMPVFDPATNRWNLFY
                     VAYAASPDTSKEWLTNHEGRIWRAVSERPGMEGINGPWKDAGIVLERGKDSDSWEGLQ
                     GTDSFFPYRVNDKWYAFYGSAHTESLPIALWQVGLAAASDLSGPWHRCTELNPLQVEK
                     RFIENPIVTRLTHGGWIAVYDNHVLNEIGYTVSEDGIHWSAGQHLVVQSGDGVWATEV
                     RTPLGLVEEKGQTFTLFYTANQKVAGAQTDGYGVNLTPGALGYAEVRLREGGQASGSL
                     HGPLWNTNPRLHAAVTKVIEGRDLSGPSSKGGRYE"
     gene            5236..6561
                     /locus_tag="VHE33_05365"
     CDS             5236..6561
                     /locus_tag="VHE33_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96916.1"
                     /translation="MTSVHDVPRPSISVSQFEKREELRRVLESRQFAAAPKRARFLEF
                     VCEQTFLGNGEKLNEYLIGVEVYGRGTEFNPQKDPIVRVQAHEIRRCLRKYYEENAAE
                     SALRIDLPAGHYIPVFSRSAPETADPEKTEPPALGPPQRPSRVHLFVTTGLALACAAL
                     VIALIYAVRRTPATIPARAAVPPVAEGLQWFWAPFLPPAHAPLIVIPNHPVLRAAHNG
                     DSEETIAHGHEIPKADLPEFRDTIHFRELRRFIFVPSTTDFTAVGEALGVVRLCEMFF
                     RVDETCRVQPSRLVNFEEIRNGNAILLGGSQAWSGRVFLETEGFHLQGGVILNRNPQA
                     GEKSEYKPEFDPVSNQLTRDYALVLMLPNQERENRVLLIYGIYTQGSQAAIEYLTDPE
                     RMAILRRALVAASPDKKSLPTYFQALITTTVENAVPGTTSLVAVRRIPN"
     gene            complement(6588..7733)
                     /gene="solA"
                     /locus_tag="VHE33_05370"
     CDS             complement(6588..7733)
                     /gene="solA"
                     /locus_tag="VHE33_05370"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HVT96917.1"
                     /translation="MARTSFDVIVLGLGAMGSAAVYHLASRGARVLGIEQFTPAHEQG
                     SSHGGSRIIRQAYFEGPDYIPLVLRAYELWRKLEADSGTELLHNTGGLTLGSSDGELI
                     SRTVAAARAHSIPYERFDAAEVRRRFPPIAPLAGDAAVLEPHAGYLLPEACIRAHLNL
                     AERAGAELQFEEKVLEWSAGGGRVSVRSSKRTYEAGHLVIAAGPWANEALRDIFALRV
                     TRQVVAWIAPRDGVRPFLPERFPVFLMEQAGGRPGYGVPALDGPSGGMKVAVHGSDDV
                     CTPGTVERTIRAADIERIRQQLSVRIPALLHGELLRAQTCLYTTTPDENFVIGAHPQA
                     ANCTVACGFSGHGFKFAGVVGEVLADLATTGTTRHPIGLFDPARLRG"
BASE COUNT         1502 a         2683 c         2364 g         1647 t
ORIGIN      
        1 acggtgacgt tctcgttggc ttgtcccggg cagcgcagag cggaagtcgc tccgcgcggc
       61 ggcaaaacgc cgggcgccgg attgctggct accgtgctgg gcatatagaa gtttgttgcc
      121 ggtggcgtga cgtcgtcccc gatcggcacg tatttatgcg gatcggaagt tttgcccccc
      181 gcgacgttaa agatcgggta gatgttcccc cacagcggcc agtacgggtt ggcagcgctg
      241 atcgagaatg gctgcccggt gtagtaatcc accgtccccg ccagcgtcca gcctccgata
      301 aacccattgg ccacgcggct ggtgtttccc agccactgct gtcctctccc aaacggcagt
      361 tggtagctgg caaagccctt caccacatgg gtcaggtcgt agccggtaat cgtgtgcgcc
      421 gcctgcgaca tcttgccgaa gtcctgaatg ggcgtgtaga acccgttgta ctcctgctgt
      481 gccgagaagg aatcgccttc ggttttcgac cacacatagt tcatgtccat ggtgaggcct
      541 ctgccggcgc gcttcaccac gtcgacgacc atcgagtcgt agaagctctg cccgagcggc
      601 agccccgcat actgcaggtt gggatagtac caatactcca cttcggcagc cgccatgtag
      661 ggaatgggag cgagcgccgc cagcggaggt ccccagaatc caggataggg gtacggcact
      721 ccgtaggcag cggcatcggc ctcgctgtag atatattgcc cgtagtccca gatattcatg
      781 tcgggatggg ctttcagcag cgccaggaac ttatccgtgc tcgtctcatt ccaggccagc
      841 gccgcatcgg tcaggcggtg gccgcggttg ccgacgtagg agatttccag ccgggtgttg
      901 ggggtgagtt gttgctgaat acccgcgttg agcgcttcgc tgtagcccag ccggagcgca
      961 cgcggatcca tgctgacggg gaacggggtc atggtggaag gatccgtatt gagtgttgct
     1021 ttgctgaaca caccgggata ttgtccgccc cctcctggcg cgtcccagct gaagttgttg
     1081 gcggcactcg agcccagttg tggagcaaat ccgttcggca cgccggtaaa gaacgtcacg
     1141 ccgaccgggt tgtagatgag gccgaacgat gcgtgcagga cggtctttgg gtgaacctga
     1201 tacgccagtc cgatcgtcgg tccgaagttc aggtagtact cgttcttctc gaagctgtcg
     1261 ctgccgtcct tcgcaaagac cagcgctccg ggaatcccat agacaggact cgtcactgtg
     1321 ggatcgaagt tcgcccagtt gccgttcttc tcatggaagc ggccgttgta gttccagcgc
     1381 aggcccagat tcgcggtcag cctgctggtg accttgtaat tgtcctgggc gaagagcgcg
     1441 agcgacttct gccggccgta gaggttgtaa ggaaccgact cgctgccctg gttgacctgc
     1501 ccgagcatgt aggtcgcgaa gccgaagccg tcgtacggat aacccggccc agcggtattt
     1561 agatacgaga agctgaagtt gtaggcgccc gatccggagt ggctgttgat ctggtgcgca
     1621 tcgaaaacgc ccccgaagct caggttgtgc cgtcccttcg tccaggtgag cgtgtcgccg
     1681 acggacgcat aggtcccctt tgtgtttccc tgccaggtgc ttcccagaaa cgtctcgctg
     1741 tgcccatagg agccattgtc ggaaaacgaa atctgcggga aattgttggc tccggtatcg
     1801 gcaaacccaa tctgcgaatt ccagtttcct gggtcggccg gatcgcttcc gttgaaatcg
     1861 aagttgtacc caaaattcaa gacgttcagc atctgcggcg taaacgtgtg ctgctcgctc
     1921 agtctccact gctgctgccg gtacatctgt acgcgcgcat tggaaagggg tccgccgctt
     1981 tgtgttccct cctgccacac gccgccgccg tcgtcgaggg tgcgcggctt gtgatcgtaa
     2041 atccaggagc cggagaggcg atcctggtcg cgcagcacgt gatccagctt cgccaccacc
     2101 cagatgggcg tttgcgtggg cgtattggag atcagcgtcc gggcgttgtt gtcgagcccg
     2161 cccagctgag gcttgtagtt cgcgtagtag gcgtttacct tctgcgctac cgggctgatg
     2221 cggcttggcg gaatgatgtt cccggtgaac tggttcccgg tgtcggggtc gtagatcatg
     2281 ttctcgcgcg cctggagctg gttgccggcg ttatcggtga ccatgatcgg agtgccaacg
     2341 gtgttcttgt gagcctgcgt gttgctgcac aggtcggtcg aaccgccaaa gtcgcagaga
     2401 gtcgggccca tcaggctgct gaagtcgccc gtcagaaatg cagggagcgg caccgtcgct
     2461 ccgccgctgc ccagccgaaa gtcaatcgac tggtaccgct cgaaggtccc gaagaaaaat
     2521 gtcttgtcct tgtggatcgg gccgccgatg ctgccgccgg tatcccacgc acgctccttg
     2581 ggcttgccca gtccaaggct gtcgttcgtc caggtgttcg cgtccagcag ctcgttgtgc
     2641 ccgtagagaa acgccgatcc gtggaacttg ttggtccccg atttcaggtt gaacgacatc
     2701 acaccgccgc cggtgatgct gctctctgcg tccagaccgc tggtctgcgc ctgcaattcc
     2761 tcgacggctt ccatgctcgg accgccgtcc atcgaatcgc cacgcacgct gctcgtcccc
     2821 gacgtgccgt caatggtgta gtccttggtg aaccactggc cgccgttcac caccgcgccg
     2881 tagggcgagc tgatcggcga ataacctggc gtaatcgcga ccgcaaagtc ttccacgaat
     2941 cgcccaccgc catagatgct cagcggcaga tcgttcacga tggtggcttt catgttggtc
     3001 ccgaccgccg cggactccat gtccagcacc ggcgcgccgc cactgacggt gacggtcgca
     3061 gtaaccgcgc ccacgtgcag cgtggcatcg atgcgcaccg cctcggtgga gttgacggtg
     3121 atatcgggat gcgtcagcgt ctcaaatccg tctcgacgga attccaggct gtacacgcca
     3181 gggggcagat tcggcacaac gtagatcccc tccgagttgg aaaccgtggc ggtggggatg
     3241 ttggttccgg tattggttgc ggttaccgcg accccggcga cgacagcgcc cgatgggtca
     3301 gccacggttc cggtgatcga gccccggttg gcctgcgccc aggcgccctg cgccatcagc
     3361 agaaccgcca ccgcgaggcc aagaccgatc ggcatggccc actttgcaaa acttttcgta
     3421 tcccggttcc ttgtcatagt gctgccccca aaagatcttt cctttgtcgc aaactcgttt
     3481 ttgtacggct ccagtcccgt gcggcgcgat ccgcagaacg ggtccatggg caactcgaac
     3541 ctttcattca taccttccgc ccttcgagga tggaccggac aaatccctgc cctcgatgac
     3601 tttcgtcact gcagcatgca ggcgcggatt tgtgttccaa agtgggccat gaagggagcc
     3661 tgatgcttgc ccaccctcgc gcagccgcac ctccgcatac ccgagagctc ccggcgtcag
     3721 gttcacccca tagccatcgg tttgcgcccc cgcaactttc tggttcgcgg tatagaacag
     3781 cgtgaacgtc tggccttttt cctcgaccag acccagaggc gtccgtacct cggtcgccca
     3841 gacgccgtcc ccgctctgca ccacgaggtg ctgccccgcg gaccagtgaa ttccgtcctc
     3901 cgacaccgtg tacccaatct cgttcagcac gtgattgtca tagacggcga tccacccgcc
     3961 atgggtcaac cgggtgacga tggggttctc gatgaatcgc ttttccacct gcagaggatt
     4021 gagttccgtg cagcgatgcc aggggccgct caaatcggaa gccgctgcaa gccccacctg
     4081 ccacagcgcg atcggcaggc tctctgtatg cgcgctgcca taaaacgcat accatttgtc
     4141 gttcacgcgg taggggaaaa acgaatccgt gccctgcagc ccttcccagg aatccgaatc
     4201 cttgccgcgt tccagaacga tccccgcgtc tttccacggt ccattgatcc cttccatgcc
     4261 cggacgctcc gatacggccc gccagatgcg tccttcatgg ttggtcagcc attccttcga
     4321 agtatcgggc gacgcggcgt acgccacata aaacaggttc caccgattcg tcgccggatc
     4381 gaaaaccggc atcggcgacc acagggccgc ccgcggatcg cggcccgtaa agtccccgct
     4441 cgattcgaac agcgtatcga cccgttgcca gtgcaggcga tccgtgctgg tccagtgggc
     4501 cagcttcatc ttcacccagt gcggttcccc cgtcatctcc gaggtgaaca ggtgatacgt
     4561 cgcccccagc ttcagaaccc tgccaccttc aaagccgtac ttgttttcgc cggcgcccgg
     4621 agaatgaatc gtcaacaccg gccgagcgca ttgccgcacg acctccatcc gaaccctcga
     4681 acccgggccc cccgaaccat cggcggtcat gctcgctgcg ggaagcagaa gagcgacact
     4741 gaacgccaga cgtctgccga tgtcgtgcat gtgcgatggc ccccgggcct tccggagcag
     4801 ataagcccca cggatgtccg ccctcacttc tatccaccat ggcctggcat ccgaaaggta
     4861 aaagaggtta tgggggcggt tagtatgcca aaaagggcag ttgccgacgc tgccgcgctt
     4921 ttcccagggc agttcggaac gacaaacggt cgctccgacc ttcctggcca ggaaagtcag
     4981 cgaagacggg cgaccaccct ggcctggacc atccccgatt ggctgaccgg gtgaggcttg
     5041 catggggtca actcttctgc tttggggcgc tgcagagcgg ccggttaacg acaaataacg
     5101 atgcagataa ccgttagtcg tgcccttttc gctttccagc ccacgggttt gaccctgcgg
     5161 gggcgcgatt gctagaattc gcccgaatgc atgtgcctcc tgcgggagcc ggtgttcgta
     5221 tccgcgaggc caggtgtgac ctccgttcac gacgttccgc gtccgtccat ctccgtctct
     5281 cagtttgaga aacgcgaaga gctgcgccgc gttctcgaga gccgccagtt tgcagccgct
     5341 cccaagcgcg cccggtttct ggaattcgtc tgcgagcaga ccttccttgg caatggcgag
     5401 aagctgaacg aatacctcat cggtgtcgaa gtctacggcc gcggtacaga attcaacccg
     5461 cagaaagacc ccattgtccg ggtccaggcg cacgaaatcc gccggtgcct ccgcaaatac
     5521 tacgaagaga acgcagccga aagcgccctt cgcatcgatt tgccggccgg ccactacatt
     5581 cccgttttca gccgcagcgc gccggagact gccgacccgg agaaaaccga accgcccgca
     5641 ctcgggccgc cgcaacgacc gagtcgcgtt cacctgttcg tgaccacagg acttgccctc
     5701 gcctgtgcgg ctctcgtcat cgccctcatc tacgccgttc gcaggacccc cgccacgatt
     5761 ccggctcgcg cggctgttcc gccggttgcc gaaggtttgc aatggttctg ggctccgttt
     5821 ctgccccccg cccacgcgcc gctgatcgtc atccccaacc atcccgtcct gcgcgctgct
     5881 cacaatggcg acagcgagga gaccatcgcg cacggccatg agattcccaa ggccgacctt
     5941 cccgagttcc gcgacacgat tcacttccgt gagctgcgcc gcttcatctt tgtcccttcc
     6001 accacggact tcacggcggt cggcgaggcg ctgggtgtgg tgcgcctgtg cgagatgttc
     6061 ttccgcgtcg atgagacctg ccgggtccag ccctcgcgcc tggtcaactt cgaagagatt
     6121 cgcaacggca acgccatcct gctcggcggc agccaggcat ggagcggccg tgtgttcctc
     6181 gaaaccgagg gttttcacct gcagggggga gtgatcctca accgcaatcc ccaggccggc
     6241 gaaaagtccg agtacaaacc cgaattcgat ccggtttcga atcagctcac gcgcgactac
     6301 gccctggtgc tcatgcttcc taatcaggaa cgcgaaaacc gcgtcctgct catctatggc
     6361 atttacacgc agggctccca ggccgccatc gagtatctga ccgatccgga gcgcatggcc
     6421 atcctgcgca gggcgcttgt cgccgcgtcg ccggataaga aatcgcttcc aacctatttc
     6481 caggcgctga tcactaccac cgtcgagaat gccgtgcccg gaacgacttc cctggtcgcc
     6541 gttcgccgca tccccaacta gactcgaccg tcagagcatt cgttggctca gcctcgcaag
     6601 cgcgctggat cgaacaggcc aatcggatga cgcgtcgtgc ctgtcgtcgc caggtcggcg
     6661 agaacctcac ctacgacgcc cgcgaattta aacccatgcc ctgagaatcc gcacgctacg
     6721 gtgcaatttg cggcctgcgg atgcgccccg atcacgaaat tttcgtccgg tgtcgtcgta
     6781 tacaggcacg tttgcgcgcg cagcagctcg ccatgcaaca gcgccgggat ccgcacagaa
     6841 agttgctgcc gaattcgctc aatgtccgcg gcgcgaatcg tgcgctccac ggtgccgggg
     6901 gtgcacacat cgtcgctacc gtgaaccgcg accttcatcc ctcccgacgg cccatcgagc
     6961 gcgggaacac cataccctgg ccgtcctccg gcctgctcca tcagaaatac aggaaaccgt
     7021 tcgggcaaga acggccgaac gccgtcacgc ggcgcgatcc acgcgaccac ctgtcgcgtc
     7081 acacgaagag caaatatatc ccgcaacgcc tcgttcgccc aggggccggc tgcgatcacc
     7141 agatgtccgg cctcatacgt tcgttttgaa ctgcgcaccg agacacgtcc tccgccggcg
     7201 ctccactcca gcactttttc ttcaaactgc agttccgcac ccgcgcgttc ggccaggttc
     7261 aaatgagcgc gaatgcatgc ctccggcagc aggtatcccg cgtgcggctc cagcacagca
     7321 gcatcaccgg cgagcggagc gatcggcgga aacctcctcc gcacttccgc cgcgtcgaac
     7381 ctctcatacg gaatcgagtg ggctcgcgcg gccgctaccg tccgcgagat gagctcgcca
     7441 tcgctcgatc cgagcgtcag tcctccggta ttgtgcagca gctcggttcc ggagtctgcc
     7501 tccagcttcc gccacaattc atacgcgcgc agtaccagcg gtatgtaatc gggtccctcg
     7561 aagtaagcct gccggatgat ccgcgagccg ccatgggagg acccctgttc gtgcgccggc
     7621 gtgaactgtt cgattcccag cacccgcgcc ccgcgcgaag ccaagtgata cacagcagcg
     7681 ctccccatcg cgcccaggcc cagcacgatg acgtcgaacg aagtccgcgc caagggttcc
     7741 tcccgattcc tgctcgtcct ttcgcgctcg cgcatgcagg cagctggaga ataaaggatg
     7801 ctgcctcgcc tgcatattcc agacggcgaa cctccccaaa agccagcttc gctcttaaag
     7861 cccgaaccgc caggaaatca atcacatagg cccttgtcag cggcttttgg atctcgaaga
     7921 cgtggcgaaa attcgtgccg ccgagcgcgg ctgcgctcga tttttcgcac atcctttgcg
     7981 ccagggctgc cctcttaacg gagaagttac ctgtgtattg cccaattcgt aaccaaggag
     8041 ttttgagtct tgctcaaacg ggctggaatt ctcgtcgttt tgtttctccc aacccttgcg
     8101 atggcccaaa gtccggagtc ggctgtcggt ggaaacagca gtttctgggc cggcggcgaa
     8161 atctcgtcct tcaatcctga ctacagctgc tccagc
//
LOCUS       DASZYN010000207         3916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_8532, whole genome shotgun sequence.
ACCESSION   DASZYN010000207 DASZYN010000000
VERSION     DASZYN010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8532"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /gene="cysE"
                     /locus_tag="VHE33_05375"
     CDS             complement(<1..708)
                     /gene="cysE"
                     /locus_tag="VHE33_05375"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF041874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HVT96918.1"
                     /translation="MFARLREDIRSILDRDPAANSALMVLLCYPGLHAVWMQQITSGF
                     WRANLRLIARFLSQVARILTGIEIHPGATIGRRLFIDHGSGVVIGETAIVGDDVTLYQ
                     GVTLGGTGHDRGKRHPTLRDGVFVGSNAQILGNICIGENSRVGAGSVVLRDVPPNSTV
                     VGVPAHIVYQNGERVLITDPNQVKDPLSGVIVALAEEVKRLRLRVDVLDGQLELATRD
                     LDEVRAFSAEDEERDRYH"
     gene            complement(776..1759)
                     /gene="cysK"
                     /locus_tag="VHE33_05380"
     CDS             complement(776..1759)
                     /gene="cysK"
                     /locus_tag="VHE33_05380"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HVT96919.1"
                     /translation="MKIANSVVDLIGNTPLVQLNRVTAGAHARVVAKLEGANPANSVK
                     DRIGFAMVEAAEREGKIHPGKTVLIEPTSGNTGIGLAFVAAVKGYKLILTMPDTMSME
                     RRVLLLAFGAQLVLTPGPNGMNGAISKAKQILEQTPDGYMLQQFENPANPKIHYNTTG
                     PEIWNDTEGQADILVAGVGTGGTLTGVGQFIKPRKPSFRVVAVEPTDSAVLSGGKPGP
                     HKIQGIGGGFIPRILETKLIDEVVQVSNDEAIAMARRLPLEEGLLVGISGGAAVHAAL
                     QVARREENAGKLIVVVLPDFGERYISSVLFESLRNQAQGLPTQPVEAPVQV"
     gene            complement(1826..2569)
                     /gene="recO"
                     /locus_tag="VHE33_05385"
     CDS             complement(1826..2569)
                     /gene="recO"
                     /locus_tag="VHE33_05385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HVT96920.1"
                     /translation="MNQTQVEALVLRKWPVHEADEIVSLFTRINGRIRGVAKAAAKSR
                     RRFGGALEPMTFVTATYVEKPKQDLVRLDACEVIASPLSDPVSYLRATALAFYAEVLE
                     EAMPDHDPHEAAFRLTLAVLDHTRGDRIWMPVTYFALWITRLLGWMPSLTHCTICGQP
                     FGAGPAWFQLQSDGLVCSQDKKPSSRILAAESLALARRIFHSPIAGFADEEWPAARAT
                     DLRRFAVQALERHLERRLRAATALVKLGG"
     gene            2823..>3916
                     /locus_tag="VHE33_05390"
     CDS             2823..>3916
                     /locus_tag="VHE33_05390"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HVT96921.1"
                     /translation="MSTEYRNFLALSYEELEELNLKAKDQRRKRVAPDKLQEERLKYL
                     TDQKGIKAVTVLFSDLEGRLHMLDYDKKFLLKSFDNLTFDGSSIRGFTAQRESDLRLG
                     IDWSSFFFAPADIFGAGKVLVFGDVIDKGGTPYGGDTRGLLKTFANEQFEKNGYTLNA
                     ANEIEGFLFAGLNAERNFPVTGKFDYVNTGGYYHSLPGDPLRTFIDTVAEVQRAMGFE
                     NEKDHPEVAPSQFEINYGYGEVIAAADQIQIYKLVCRQVATRLGMTACFLPKPVVGVN
                     GSGMHTNVSISKDGKNLFWDPKGEEKLSGLGWEFVDRILTHGNDICLLLNSSVNAYRR
                     LDPHFEAPNQIKASAVDRGSMIRIPIGNERS"
BASE COUNT          761 a         1242 c         1176 g          737 t
ORIGIN      
        1 gtggtaccgg tcccgctctt cgtcctccgc gctgaaggcg cgcacctcgt ccagatcccg
       61 cgtggccaat tcgagctgcc catcgagcac atcgacgcgc agccgcagcc gcttcacctc
      121 ctcggccagt gcgacgatca cccccgaaag aggatccttc acctgattgg gatcggtgat
      181 gagcacgcgc tcgccgttct gatagacgat gtgtgccggt accccaacga ccgtcgagtt
      241 cggcggcaca tcgcgcagga ccaccgatcc ggcgccaact cgcgagtttt ccccaatgca
      301 gatgttgccg agaatttgcg cattgctgcc gacgaagacg ccgtcgcgca aggtcggatg
      361 ccgcttgccg cgatcgtgcc cggtgccgcc gagggtgaca ccctggtaga gagtcacgtc
      421 gtcgccgacg atggctgttt cgccgatcac cacgcccgag ccatgatcga taaacagccg
      481 ccgcccgatc gttgcgccgg ggtgaatctc aatcccggtg agaattcgtg ccacctggga
      541 gagaaatcgg gcgatcagcc gcagattggc ccgccagaaa cccgaggtga tttgctgcat
      601 ccacaccgca tgcagtcccg gatagcacag cagcaccatc agcgcggaat tggccgcagg
      661 atcgcgatcc aggatcgaac ggatgtcttc gcggaggcgg gcgaacatgg aatcagcgta
      721 tagcggttag cgggcagcgt atagcgcttg tctgccgggc actgaccacg gccgcctaga
      781 cctgcaccgg tgcttccacg ggctgggtcg gcagcccctg cgcctgattc cggagactct
      841 caaacaatac cgacgaaata tagcgctcgc cgaaatccgg aagcaccacg acgatcagtt
      901 ttccggcgtt ctcctcgcgg cgtgccacct gcagggcggc gtgtacggcg gctccgccgg
      961 agatgccgac cagaagcccc tcttccaggg gaagccgacg cgccatcgca atcgcctcgt
     1021 cgttggaaac ctgcacgacc tcatcgatca gtttggtctc caggatccgg ggaataaagc
     1081 ctccgccgat tccctgaatc ttgtgcggcc cgggcttgcc gcccgagagc actgcgctgt
     1141 cagttggctc caccgcgacg acacggaacg atggcttacg cggcttgatg aactggccca
     1201 caccggtgag cgtgccgccg gtgcctacgc cggctaccag gatgtcggct tgtccttcgg
     1261 tgtcgttcca aatctccggc cccgtcgtgt tgtagtggat cttcgggttc gccggattct
     1321 cgaattgctg cagcatgtag ccatcgggcg tctgctcgag gatttgtttc gctttggaga
     1381 tggcgccgtt catgccgttc ggtccggggg tgagcacgag ttgtgcgcca aaggcgagca
     1441 gcaacacgcg ccgctccatg ctcatcgtgt ccggcatggt gaggatcagc ttgtacccct
     1501 tcacggccgc gacgaaggca aggccgatcc cggtattgcc gctggtgggc tcaatcagaa
     1561 ccgtcttgcc agggtggatt ttgccttccc gctccgccgc ttcgaccatc gcgaagccga
     1621 tgcggtcttt cacgctgttg gccgggtttg cgccttccag tttggccacg acgcgggcgt
     1681 gcgcgcctgc cgtcacgcgg ttcaactgca ccagcggcgt gttgccgatc agatcgacga
     1741 cgctattggc aattttcatc agtccaccct cgacggagaa agttttccag gccttcagtg
     1801 tagatgcacg aggcgaactg cggactcagc cgcccagctt taccagagcc gtcgccgcgc
     1861 gcagcctgcg ctcgagatgc cgctccagcg cctggacagc gaagcggcga agatccgtcg
     1921 cccgggctgc cggccattct tcatccgcga acccagcgat cggcgagtgg aaaatccggc
     1981 gcgcgagggc cagggactcg gcggccagaa tgcggctcga cggcttttta tcctggctgc
     2041 acaccagtcc gtcggattgc aactggaacc aagccggccc ggcgccgaac ggctgaccgc
     2101 aaatcgtgca gtgcgtcaga gagggcatcc atcccagcag gcgggtgatc cacagcgcga
     2161 aatacgtaac cggcatccag atgcggtccc ctcgggtgtg atccagtaca gcgagagtca
     2221 gacggaaggc cgcctcgtgg ggatcgtgat cgggcatcgc ctcttcgagg acctcggcgt
     2281 agaaggcgag ggcggtcgcg cgcagatagc tcaccggatc ggacagcggc gacgcaatca
     2341 cctcgcaggc atcgagccgg acgagatcct gcttcggctt ctcgacatag gtcgcggtca
     2401 caaaggtcat gggctccagc gccccaccga accggcgccg cgatttggcc gctgccttgg
     2461 ccaccccgcg aatccgcccg ttgatccgcg taaacagcga cacgatttca tcggcctcgt
     2521 ggacgggcca cttgcgcagt acgagagctt cgacctgggt ctggttcatg ctgcgatcca
     2581 aaatcagatt agcgtctgcc cggagatcca gcctcatggc cgatgattcg gcccgaattt
     2641 ccacagtcgc cggttccggg aagggaactt tctcgctgcc gtgcgcgata ccctgaatgg
     2701 aagctgctca gcccacttgt ttgcgctgta tatccggcct gtcggcgcga atcaccgctg
     2761 tccatcagtc tgatcgctta cgggctgcat caccgactcc aatcacgatt cagaggagaa
     2821 gcatgtcgac cgaatatcgg aatttcctgg cgctttcata tgaggaactg gaagagctga
     2881 atctgaaggc caaagatcag cgcaggaagc gcgtcgcgcc tgacaagctt caggaagagc
     2941 gactgaagta tctgaccgac cagaagggga tcaaggcggt caccgtgctc ttttccgatc
     3001 tcgaaggccg gctccacatg ctggactacg acaagaagtt cctgctgaag agcttcgaca
     3061 acctgacctt tgacggttcc tcgatccgcg ggtttaccgc gcagcgggag agcgacctgc
     3121 gcctcggcat cgactggagc tcattctttt ttgcgcccgc cgatatcttc ggcgccggca
     3181 aagtactggt tttcggcgat gtcatcgata agggcggcac gccttatggc ggagacacgc
     3241 gaggattgct caagaccttc gccaacgagc aattcgagaa aaatggctac acgctgaacg
     3301 cggcgaacga aatcgaaggc tttctctttg ctgggctcaa cgcggaacgc aatttccccg
     3361 tcaccgggaa gttcgactac gtgaacaccg gcggctacta ccactcgctg cctggcgatc
     3421 cgctgcggac cttcatcgat acggtcgccg aagtgcagcg cgccatgggc ttcgagaacg
     3481 agaaggacca ccccgaagtc gcgccgtcgc agttcgaaat caattacgga tacggcgaag
     3541 tcatcgccgc ggccgaccag attcagatct acaagctggt ctgccggcag gttgcgacca
     3601 ggctcggcat gaccgcgtgc ttcctgccca agccggttgt cggggttaac ggcagcggca
     3661 tgcacacgaa cgtctcgatc agcaaagacg gcaaaaacct cttctgggat ccgaaaggcg
     3721 aggagaagct ctccgggctg ggttgggaat tcgtggaccg catcctgacc catggcaacg
     3781 atatctgcct gctgctgaac tccagcgtca acgcctatcg ccgccttgat ccacactttg
     3841 aggcgccaaa ccagatcaag gcttctgcgg tggatcgcgg atcgatgatc cgcattccca
     3901 tcggcaatga gcgttc
//
LOCUS       DASZYN010000208         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_648374, whole genome shotgun sequence.
ACCESSION   DASZYN010000208 DASZYN010000000
VERSION     DASZYN010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648374"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VHE33_05395"
     CDS             complement(<1..226)
                     /locus_tag="VHE33_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96922.1"
                     /translation="MTKNQKTPPRNGWLKALDQTLGTGRLSAGRMGAMDENPYRAPKE
                     EMAQPATPLDGAEPTLLGLGLTLGLVIAWAI"
     gene            693..2015
                     /locus_tag="VHE33_05400"
     CDS             693..2015
                     /locus_tag="VHE33_05400"
                     /inference="COORDINATES: protein motif:HMM:NF033540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HVT96923.1"
                     /translation="MDARALRRLKPELDSFLDRYVPLFGRAENYEHARRFVQGLLAGG
                     ERRNVENIAEQVSGGVVRTLQKFVAQGVWEDRDVLSELRRDIASRWGDEEAIINVDET
                     GFPKKGTKSVGVKRQYAGILGRTDNCQVGVFLNYVTHQGHALIDRRLFLPQEWAEDGA
                     RRREAGVPEAVVFRTKPELALEMIQEAVSAGVPFRWVGGDCLYGDSPKFVQGVRALGK
                     WYVLDTSMDAYVWTTAPQLRPPTTHSERGGRPRTRLVATNKPQPISVVIATIPEKVWK
                     RITVAEGSQGPRIYEYTELTVWFSEEGSPTVEPERLLVRRSVGQEAEIKVQRSNAPHH
                     VPLSRLAQVGGCRWTVEEDFQCGKGECGLDEYETRGWRGWHHHTALSMLALWFLAQQK
                     ERLGEKRASNDRTRSPRPLAASARPAQVGRARNPRLVRMAAGTQPHRR"
BASE COUNT          453 a          658 c          755 g          479 t
ORIGIN      
        1 agattgccca agcaatgacg aggcccagcg tgaggccgag gccgagcagc gtcggctctg
       61 cgccgtcgag aggggtggcc ggttgagcca tctcctcttt cggcgcccga tacggattct
      121 cgtccatggc gcccattcta ccggccgaca gccgcccggt tccaagcgtc tggtccagtg
      181 ccttgagcca cccgttgcgc ggcggtgttt tctgattctt ggtcaagagc tgccgcgatt
      241 tcctctccga ttgagcctgc gcgtcccgtc atgttaaagc acaatcgccc atttagcccc
      301 tcccaggcat caaaatgccc gatcgcatcc tggaatccag cagcatgtgc tcgcactgct
      361 aagcgatagc tgccatgcgg gatttcttct tcgggcaggt gccccattgc gaaacgaagg
      421 aaccactctc ctgacggcaa ttggcgaact atgtcgtaat ctgtggagcc gtcgggcaca
      481 tcgtgcgtcg tctcccattc tttgtgacgg tgaatcattt cgagtggccg cgcccgcatg
      541 ttgtccagag cgttttggag aagtttgtcg acactgtcta gtactacagc gcctttttaa
      601 ttgatctttt acgctggcct gtgggtcggg tacaagagca gtccacggta cttgcggcag
      661 gtgttgctct tttgcttgca tggaggtaga gcatggatgc tcgtgcactg cgtcggttga
      721 aacccgagct ggattcgttt ctggatcgat acgttccttt gtttggtcgc gcggagaatt
      781 acgaacacgc gcggcgattc gtacaaggtt tgctggcggg cggcgaacga cgcaacgtgg
      841 aaaacatcgc cgagcaggta tccgggggcg tcgtgcgcac gctgcagaag tttgtagccc
      901 agggcgtgtg ggaagatcga gacgtcctgt cggaattgcg gcgggatatt gcgtctcgct
      961 ggggagacga ggaggcgatc atcaatgtcg atgagaccgg ttttcccaag aaaggaacca
     1021 agtcggtggg ggtgaagcgg cagtacgccg ggatcctggg gcgaacggac aattgtcagg
     1081 tgggggtgtt cctgaattat gtgacccacc aaggtcatgc gctgatcgat cggcgattgt
     1141 ttctccccca ggagtgggct gaggacggtg cgcgtcggcg ggaagctggc gtgcctgaag
     1201 ctgtcgtgtt tcgcaccaag cccgagctgg ccttggagat gatccaagag gccgtaagcg
     1261 ccggggttcc ttttcgctgg gtcggtggag actgcttgta cggcgacagc ccaaaattcg
     1321 tacaaggggt gcgggccctc ggcaagtggt acgtgctgga cacgtcgatg gatgcctacg
     1381 tttggaccac cgcaccccaa cttcgacctc ccacaacaca cagcgaacgt ggcggccgcc
     1441 cacggacgcg cctggtggcg acgaacaagc cgcagccgat ttcggtcgtg atcgcgacga
     1501 tccctgaaaa ggtctggaaa cggatcacgg ttgctgaagg cagccaaggt ccgcgcattt
     1561 atgaatacac ggaattaacg gtctggttct cggaagaggg atcgcccacc gtagaacctg
     1621 agcggttgtt ggtgcgccgc tccgtggggc aggaggcgga gatcaaagtc caacgcagca
     1681 atgctccgca ccacgtcccc ctgagtagat tggcccaggt cggcggttgc cgctggacgg
     1741 tcgaagagga cttccagtgt ggcaaaggcg agtgcggatt ggacgagtat gagactcgtg
     1801 gctggcgcgg atggcaccac catacggctc tgtcgatgct ggccctgtgg ttcctcgctc
     1861 aacagaagga gcggttgggg gaaaaaagag cctcaaatga ccgtacccga agtccgcgcc
     1921 ctcttgcggc atctgctcga cctgcgcagg tgggacgagc gcgaaatcct cgcctggtcc
     1981 gcatggcggc aggaacgcaa ccgcatcgcc gctgagtgcc acgccaaacg acgagcccga
     2041 gccaagcgta aaagatcaat aaaaagggcg ctgtagtact aggcgggtaa cctcatggat
     2101 tttgcattcg agcttgtcga taggaccccc gtcagtcgcc gataggctgt aaggtccctc
     2161 tcctgccccg attggcggat gagcggatgc gaatcttgct cgtagcggcg gcgggccgcg
     2221 gcgccgtcgc cgctagagca acatctctca cgccgtcgcc ttgcgggccg cctcgcgcag
     2281 atagtcgggc gcgtggctga ggtgggaata gaccttcgtc accatcgagc agtcggcatg
     2341 gccca
//
LOCUS       DASZYN010000209         5229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_390567, whole genome shotgun sequence.
ACCESSION   DASZYN010000209 DASZYN010000000
VERSION     DASZYN010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390567"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..721)
                     /locus_tag="VHE33_05405"
     CDS             complement(176..721)
                     /locus_tag="VHE33_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96924.1"
                     /translation="MNRNAGQSAVDRLIALAQQKPGRPGAASMEESTDIETVPLGLPS
                     MRELEHNPMWKALLQLRAFLPYVARLLEMSSPSQHSTALTTELRSEVRQSVGALESSH
                     RDLRLAVQDQTVEIKRIEEAVTKSREATERNALEVTELAEDVRSMRSLVKTGIILLVL
                     LFLTLIGTVVFLLFRIKPLLH"
     gene            930..1604
                     /gene="pgsA"
                     /locus_tag="VHE33_05410"
     CDS             930..1604
                     /gene="pgsA"
                     /locus_tag="VHE33_05410"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HVT96925.1"
                     /translation="MNLPNSITMGRILGIPVLIWMLSPRFPHGSFHGEQEIIASLFFI
                     LLSISDGVDGYLARSRGQVTTMGMLLDPIADKLMITSAYVCLVEMNPHVVKAWIVVVV
                     IGREFLVSGLRSIASTEGFTIEASDVGKLKTVIQIVSVVAATLDHGWYVWHWGWFIVP
                     VDWFAVVGIYYTAAVSIISAIDYFVAFWRKIDKATAKQRSRDNEYVLSRRKKNSPAAA
                     HNATQL"
     gene            1732..2415
                     /locus_tag="VHE33_05415"
     CDS             1732..2415
                     /locus_tag="VHE33_05415"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVT96926.1"
                     /translation="MDHPVKPPNNLRNSEAEEKQPEEQKRDSSFGRFSRRSFLAQLGA
                     AGVAATASPLAHAAVSETGEEAASAVPGAVPVTLNVNGKEHKLRLEPRVTLLDALREH
                     LQLFGTKKGCDHGQCGACTVHVNGRRVNSCLSFAVMHQGDKITTIEGLAQNGQLHPVQ
                     QAFLDHDGFQCGYCTSGQIMSGAALMHEPIAADDNAVRVAMSGNICRCGAYTNIVAAV
                     QQARKSTTV"
     gene            2426..3397
                     /locus_tag="VHE33_05420"
     CDS             2426..3397
                     /locus_tag="VHE33_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HVT96927.1"
                     /translation="MEVFRYTPARSVDAAVRSAGTYIAGGTTLVDLMKLNVLQPQSVV
                     DINGLPLDRVEALPNGGLRIGALVRNSDLANHEAVKKNYAVLSQALLSGASPQLRNMA
                     TTGGNLLQKTRCYYYRDVNYACNKRQPGSGCAALDGFNRIHAVLGGSDHCVATHPSDM
                     AVAMMALEATVHTQGPKGERSIALDEFYLVPGSTPDRENVLAPGELITYVTLPALASG
                     ARSVYLKLRDRQQYEFALASSALVIHVSGGRIERARVAFGGVGTKPWRSHEAEKALEG
                     QQATPETFQRAAEAAMTGAKPLKYNAFKIELAKRALVRTLEETTQAA"
     gene            3409..>5229
                     /locus_tag="VHE33_05425"
     CDS             3409..>5229
                     /locus_tag="VHE33_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HVT96928.1"
                     /translation="MSTSIIGTSVTRVDGPLKVTGKAAYALDHPLENVAWGVPVASTV
                     GKAKITRIDSSAAERMPGFLAILHHGNIDPLFRPAGQWEHSRASEARPPFEDDNVYYY
                     GQYVALVVANTFEQAKAAADAVKVEYAAQKPSVSFDDGPTPDKPRVHYTRGKADPAFD
                     SAPVKIDETYEISTETHNPMEMHATIAAWDGDKVTLYETSQGVMNHQQVMSEMLGIPL
                     DRILVVSPFQGSGFGGKLFPWPHSLLAAIGARKVNRPVKVQVPRSLMFTTVGHRPMTR
                     QRMRLGANQDGKLLSIQHDVTQPTSMVDTYMEACTGVTTMLYSCENVTAQQTLVERNL
                     GTPTPMRGPGIVPGLFPLESAMDELAIKLNMDPLELRLKNYSEQDESFSPARPYSSKR
                     LRECYQAGAEKFGWSKRNPQIGSMKNGNEILGWGMATATWHAGRGSATVRVRLNADGT
                     ARATCATQDIGTGTYTIFAQIVSEKTGIPLDKIEVILGSTDLPPGPTSGGSTVTATVS
                     PAIAQATQGAVDRVIAAAQSIPSSPFHPNNLPADDPVKLTMTAGRIHAMGKPPESGVP
                     FGEILTAGKLAALENEARTAPDPAKAHEFSTHSFGAQFIEV"
BASE COUNT         1010 a         1862 c         1427 g          930 t
ORIGIN      
        1 tctgctctcc gatgttcggc tggcgctcca tgccgtgatc ctcattttcc ggtggctgag
       61 cgggtgggtt ttgcgctgcg tccgcctgcg cgttctcccc aggatacttt tccgacaagg
      121 ccatggatcc tcctgccaga ggagaggcct cgaacctgat ttgctgttgc ctggattaat
      181 gcagcagagg cttgatccgg aacagcagga acaccaccgt gccgatcagc gtcagaaata
      241 acagcaccag gaggatgatt cccgtcttga ccagcgaccg catcgaacgc acatcctcag
      301 ccaattccgt cacttccagc gcgttccgct cggttgcctc gcggctcttc gttactgctt
      361 cttcgatccg cttgatctcg accgtctggt cctgcaccgc caggcgcaga tcccggtgcg
      421 agctttccag cgctcccacg gattgccgaa cctcgctgcg caactccgtc gtcagtgcag
      481 tcgagtgctg ggagggactg ctcatctcca gcagccgggc gacatacggc aaaaacgccc
      541 gcaactgcaa cagggctttc cacataggat tgtgctccag ctctctcatc gacggcaggc
      601 cgagcggaac ggtctcgatg tcggtcgatt cttccatgct cgcggcgccc ggccggcctg
      661 gcttctgctg cgccagcgcg atcagcctgt ccactgcgga ttggcctgcg ttccgattca
      721 tgagggtcct tcagtcacta tagtcaaacc cgcagtaccc cctccatgct tctttccggg
      781 tgttaaccat caggtaccgg cccccaggct cgcggattca ggccgccgcg cacatccgct
      841 gccacccaaa gtaccgtgcg cgccacactc ccgccgggca aaccccctgt tgactggtgc
      901 ataatggctg caggggcgca tctctccgcg tgaacctccc caactccatc acgatgggcc
      961 ggattctcgg gattcccgtg ctcatctgga tgctgtcccc gcgctttccc cacggcagct
     1021 ttcacggcga gcaggaaatc atcgcctcgc tgttcttcat tctcctgtcc atctccgacg
     1081 gtgtcgacgg ctacctggcc cgcagccgcg gccaggtcac caccatgggc atgctgctcg
     1141 atccgattgc cgacaagttg atgatcacat cggcgtacgt ctgcctggtg gagatgaatc
     1201 cccacgttgt gaaggcgtgg attgtcgtgg tggtgatcgg acgcgaattc ctggtcagcg
     1261 gactccgctc catcgcctcc accgagggct tcaccatcga agccagcgac gtgggcaaac
     1321 tcaaaaccgt catccagatc gtctccgtcg tcgccgccac cctcgatcat ggctggtacg
     1381 tctggcattg gggctggttc attgtccccg tcgattggtt cgcggtggtc ggcatctact
     1441 acaccgcggc cgtctcgatc atttccgcga ttgactactt cgtggccttc tggcgcaaga
     1501 ttgataaggc gactgcaaaa cagcggagtc gcgataatga atacgtgctg agccgtcgca
     1561 agaagaactc cccagcggcc gcgcacaacg caacgcaact gtaacaatca cagccgggcc
     1621 cccaggcccg acaagcacca atcactgcta tcgatattgg atggaacgac gctgccgggt
     1681 cttttcggtc gcgccgcgcc atcgcaacat ccaggttcat caggagccca aatggatcac
     1741 cctgtgaagc ccccgaacaa tctccggaat tccgaagccg aagagaagca accagaagag
     1801 cagaaacgag actccagctt cggccgattc tcgcggcgct ccttcctcgc tcaactcggc
     1861 gccgccggtg tcgccgcgac cgcctctcct cttgcccacg ctgctgtctc cgaaaccggc
     1921 gaggaagccg cttccgcagt cccaggcgcc gtccccgtca cgctcaacgt caatggcaaa
     1981 gagcacaagc tgcgcctgga gccgcgcgtc accctgctcg acgctctgcg cgaacacctc
     2041 cagctcttcg ggaccaaaaa aggctgcgac cacggccagt gcggcgcctg caccgtccat
     2101 gtcaacggcc gccgcgtgaa ttcgtgtctc agcttcgccg tcatgcacca gggcgacaag
     2161 atcaccacca tcgaaggcct tgcacagaac ggccagctgc accccgtcca gcaggcgttt
     2221 ctcgatcacg atggattcca gtgcggctac tgcacctccg gacaaatcat gtcgggcgcg
     2281 gcattgatgc acgaacccat cgctgccgac gacaatgccg ttcgcgtggc gatgagcggc
     2341 aacatctgcc gctgcggcgc ctacaccaac atcgtggccg ccgttcaaca ggctcgcaaa
     2401 tccaccaccg tctagaggag gcgtcatgga agtctttcgc tacactccag cccgaagcgt
     2461 tgacgccgcc gtgcgctccg ctggcactta catcgcgggc ggcacgaccc tcgtcgacct
     2521 gatgaagctc aacgtgctgc agccgcagtc cgtggtcgac atcaacggcc ttcccctcga
     2581 ccgcgtcgaa gccctgccca acggcggcct tcgcattggc gcgctggtcc gcaacagcga
     2641 cctcgccaat cacgaagcgg tgaagaagaa ttacgctgtc ctctctcagg cgctgctctc
     2701 cggcgcttcg ccgcagctcc gcaacatggc caccactggt ggcaatctgc tgcagaagac
     2761 gcgctgctac tactaccgcg acgtcaatta cgcctgcaac aagcgccagc ccggctccgg
     2821 ctgcgccgcg ctcgacggat tcaaccgcat ccatgctgtc cttggcggca gcgaccactg
     2881 cgtcgccacg catccttccg acatggccgt cgccatgatg gcgctcgagg ccacggtcca
     2941 cacccagggg ccgaagggcg agcgatccat cgcactggac gagttctacc tcgtgcccgg
     3001 cagcacgccc gaccgcgaaa acgtcctcgc gcccggcgag ttgatcacat atgtaactct
     3061 gccagcgctc gccagcggag cccgatccgt ttatctcaag ctgcgcgacc gccagcagta
     3121 tgaattcgcc ctggcctcgt cggcgctcgt catccatgtg agcggcggac gcatcgagcg
     3181 cgcccgcgtc gcctttggcg gagtgggcac gaagccctgg cgttctcacg aagccgaaaa
     3241 ggccctcgaa ggccagcagg ccacgccgga gaccttccag cgtgcggccg aggctgcaat
     3301 gaccggggca aaacccctca aatacaacgc attcaaaatt gagctcgcca agcgcgccct
     3361 cgtgcgcacg ctcgaagaaa ctacccaggc cgcctgagga gacccagtat gagcacttcc
     3421 atcattggca cttccgtgac gcgggtcgac ggacccctca aagtgaccgg caaggccgct
     3481 tacgcgctcg accatccgct ggaaaacgtg gcgtggggcg tgccggttgc cagcacggtg
     3541 gggaaagcaa aaatcacccg catcgactcc agtgcagccg agcgtatgcc aggctttctc
     3601 gccattctgc atcacggcaa tatcgatccc ctcttccgtc ccgccggaca gtgggagcac
     3661 agccgcgcca gcgaagcgcg tccgcccttt gaagacgaca atgtctacta ctacggccaa
     3721 tatgtagcgc tcgtggttgc aaataccttc gagcaggcga aggcagcagc ggacgccgtc
     3781 aaggtggagt acgccgcgca gaagccctcc gtttcttttg acgacggacc caccccggac
     3841 aagccgcgtg tccactacac ccgcggcaag gcggacccgg cctttgacag cgcgcccgtc
     3901 aagatcgatg agacctatga aatttctacc gaaacccaca acccgatgga gatgcacgcc
     3961 accatcgcgg cctgggacgg cgataaggtc accctctacg aaaccagcca gggcgtgatg
     4021 aaccaccagc aggtcatgtc ggagatgctt ggcatccctc tcgaccgcat cctcgtcgtc
     4081 tctcccttcc agggctccgg ctttggcgga aaactatttc cctggccgca ttcgctgctg
     4141 gccgcaatcg gcgcgcgcaa agtcaatcgc cccgtgaaag tacaggttcc gcgcagcctc
     4201 atgttcacca ccgtggggca ccgccccatg acccgtcagc gcatgcgcct cggcgccaac
     4261 caggacggca agctgctctc gatccagcac gacgtcacac agcccacctc catggtcgac
     4321 acctacatgg aagcctgcac cggcgtcacc accatgctct acagctgcga gaacgtcacc
     4381 gcccagcaga cgctggtcga gcgcaatctc ggcaccccca ccccgatgcg cggccccggc
     4441 atcgttcccg gcctcttccc gctcgaatcg gccatggacg agctggccat caagctcaac
     4501 atggacccgc tcgagctgcg cctgaagaac tactccgaac aggacgaaag cttctctccg
     4561 gctcggccct acagcagcaa gcgtctccgc gagtgctacc aggccggcgc cgaaaaattc
     4621 ggctggtcga agcgcaatcc gcagatcggc tcgatgaaga acggcaatga gatcctcggc
     4681 tggggcatgg ccacggccac ctggcatgcc ggacgcggct ccgccacggt ccgcgttcgt
     4741 ctcaatgccg atggcaccgc gcgcgccacc tgcgctaccc aggacatcgg tacgggaacg
     4801 tacaccatct ttgcgcagat tgtttccgag aagaccggca ttcctctcga caagatcgaa
     4861 gtgatcctgg gcagcaccga tctgccgccc ggccccacct ccggcggatc cacggtgacc
     4921 gccaccgtct cgccggccat tgcccaggcc acccagggcg ctgtcgatcg cgtgatcgcc
     4981 gccgcgcagt ccatccccag ctcgcccttc catccgaaca atctcccggc cgacgatccc
     5041 gtcaagctga cgatgacggc tggccgcatt catgccatgg gcaagccacc cgaatccggc
     5101 gtccctttcg gcgaaatcct cactgcgggc aaactggccg ctcttgaaaa cgaagcgcgc
     5161 accgcccccg atccggccaa ggcgcacgaa ttctccaccc actctttcgg cgctcagttc
     5221 atcgaagtc
//
LOCUS       DASZYN010000210         4797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_519761, whole genome shotgun sequence.
ACCESSION   DASZYN010000210 DASZYN010000000
VERSION     DASZYN010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519761"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="VHE33_05430"
     CDS             <1..1209
                     /locus_tag="VHE33_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HVT96929.1"
                     /translation="PLSGSGQSPPTDTLSSLSLNFAPQQVGTTSTAQPITLTNNGDQI
                     LTGIAVAASGDFVVVNNCGTTLQAHASCSLSISYAPTVAGPESGTVTVTDNVRTQKIT
                     LSGTGVTPPGASALPASIDFGGFAVGTTSSVKTITVTNNGGYDLTGLAVSITAGFTTA
                     ANDCPNTLPVGAVCRIGVTLTPMAAGSISGSVTVTATNLLKPILVVLSGQGQDFGLAV
                     SGSSAAIVNSGQTATYALQLSGLSGMTGTVDLRCTGAPQNSTCSLNPASIAVSSANTS
                     SVTVTIATGIATSAALRPSPGNTALLWSSSARMLSLVVPLALLGVRGRRRIHGALLML
                     LLGFCVAGCGVSSSSGAGSGGGSGGGSGGTGGGGGGSSTPTPSGTYTLTVTGTMSNIV
                     HTTKLTLTVQ"
     gene            complement(1565..3259)
                     /gene="rpoD"
                     /locus_tag="VHE33_05435"
     CDS             complement(1565..3259)
                     /gene="rpoD"
                     /locus_tag="VHE33_05435"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HVT96930.1"
                     /translation="MAIEDKYEADIQKLIDAGKEKGYLTYNEVNDLLPGDMNSPDDLD
                     DLITTIGTQGIDILEGGPKIGTPGERDDFEGEEGEDVELDLTPGTLEKTNDPVRMYLR
                     EMGTVPLLTREGEVEIARRIERGQLRVLKAISRSPVVIREIVALGEDLKRGVRNIKEV
                     VIFDDEELTEEVVQTRVRATVTRVDTLVKHQKKAQQLEEKLAGVNQKAKPKEHRRLRW
                     SIAREKVLVSRIVRELKYTNLERKRLLDKVNKTVEAMRTLERQIRSLDTKFEQSRSEE
                     LKKEYRRQQKNCKTDLEKLEEEAGVTIAELKRTQREMIQGDMDAEQAKRELIEANLRL
                     VVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRAIA
                     DQARTIRIPVHMIETINKLIRTSRQLVQELGREPTSEEIAKRMDIPVAKVRKVLKIAQ
                     EPISLETPIGEEEDSHLGDFIEDRQAVSPSDAVISVNLKEYTSQVLRTLTPREERVVK
                     MRFGLEDGSEHTLEEVGQSFQVTRERIRQIEAKALRKLRHPSRSRKLRAFVDTVHD"
     gene            3737..>4797
                     /gene="dnaG"
                     /locus_tag="VHE33_05440"
     CDS             3737..>4797
                     /gene="dnaG"
                     /locus_tag="VHE33_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HVT96931.1"
                     /translation="MANDFAQSVKEQSDIVKIIGDYVRLRKAGAQNYTGLCPFHKEKT
                     PSFSVHAGRQFFNCFGCHEHGDVFTFIQKIENVGFFEAVRTVAQKSGIPLPKREFSSP
                     EEAAEHRQRAKLLDLHEAATAWFEEQLRAPEAATAREYLSGRGLTADGIAKFRIGYAP
                     DSFRALGERLQSLADPDTLRQSGLFSWKEQEDGSPGPIYSRFRKRIMFPIANEAGRVI
                     AFTARTLETGEKAGPKYLNSPETPLYSKSHVLFNLDKARQAIRQDGGAILVEGQMDCI
                     SVFLAGIPNVIATSGTAFTEPQVRLLRRYATRVIVNFDPDTAGANAAEKSIALLVEEG
                     FDIRVLTLEAGLDPDRYIR"
BASE COUNT          855 a         1749 c         1227 g          966 t
ORIGIN      
        1 cctctctccg gttccggcca gagcccgccg actgacacgc tctcgtccct gtcgctcaac
       61 ttcgcaccgc agcaggtcgg caccacgagc acggcgcagc ccatcacgct caccaacaac
      121 ggagatcaga ttctcaccgg catcgccgtt gccgccagcg gagacttcgt cgtcgtcaac
      181 aactgcggta cgactctgca ggcccacgcc tcgtgcagcc tctctatctc ttatgcgccg
      241 acagtcgccg gtccggaatc tggaaccgtc actgtgacag ataacgtccg cacgcagaaa
      301 attacgctga gcggaacagg agtcaccccg ccaggagcct cagctctccc ggcatcgatc
      361 gacttcggcg gtttcgccgt cggcacaacc agcagcgtga aaaccatcac cgtcaccaat
      421 aatggcggct acgatctcac cggcctcgcc gtatccatta ctgccggctt cacgactgct
      481 gccaacgact gccccaatac tctgcctgtt ggcgctgtct gccggattgg cgtcacactg
      541 acccccatgg ccgccggctc gatctccggt tccgtcaccg tcacagctac aaatttgctc
      601 aaaccgattt tggtcgtcct ctccggccag ggtcaggact ttggccttgc cgtgagcggc
      661 tcctctgcag ccatcgtcaa cagcggccag acggcgactt atgccctcca gctctccgga
      721 ctcagcggaa tgaccggaac ggtcgatctt cgctgcacgg gagcgccgca aaactccacc
      781 tgctcgctga accccgccag catcgccgtc tccagcgcga atacctccag cgtcactgtc
      841 accatcgcca ccggcatcgc gacctccgcc gccctgaggc catcgccagg taacacggcc
      901 cttctctgga gttcatctgc acggatgcta agtcttgttg tccccctcgc cctgcttggc
      961 gttcgtggcc ggaggcgtat tcatggcgcg ttgctgatgc tcctcctcgg cttttgtgtt
     1021 gcaggttgtg gcgtaagctc cagctccgga gccggcagtg gtggaggcag tggtggaggg
     1081 agtggcggca caggtggcgg aggcggtggc agttccactc cgaccccttc cggaacctac
     1141 acgctcaccg tcacgggaac gatgtcgaac atcgtccaca caaccaagct gacgctcacg
     1201 gttcagtaga ggcgccgctg cctcccgtaa ccccgcgcgt gtcgcccgga aggccctcgc
     1261 gaaaacgccc cccttacgcg gggaaggcca ggttcacccc acagcaagaa gctaaagccc
     1321 gtaggtcagg accttcaaca gaattgctcg cctcgtgcag tacctgagtg ctgcgtccat
     1381 cccattccgg gaaacagcag ccaccagccc ggtcggcagg ctctgttcac gaatcttcga
     1441 gacggtgtgc gtaagaaatc aaccgcgaaa catttgcgct cgactccgag ctgacccaaa
     1501 acaaaaaggc cgcccctttc gggacggcct ttaccgctga acgcgatgcg ctcaacgctg
     1561 tctcttagtc atgcaccgta tccacaaacg cccgcaactt ccgcgagcgc gacggatgcc
     1621 gcagcttgcg cagcgccttg gcttcgatct ggcgaatgcg ctcgcgcgtc acctggaagc
     1681 tctgtcccac ctcttccagc gtgtgctccg agccatcctc gaggccaaac cgcatcttca
     1741 ccacacgctc ttcgcgcggg gtgagcgtgc gcagcacctg cgaggtgtat tccttcaggt
     1801 ttacgctgat gaccgcatcg gacggcgaca ccgcctggcg gtcctcgatg aaatcgccga
     1861 ggtgcgaatc ttcctcttca ccgatcggcg tctccagcga aatcggctcc tgcgcgatct
     1921 tcagcacctt gcgcaccttc gccacaggaa tatccatccg cttcgcgatc tcttccgagg
     1981 tcggctcgcg tcccagctcc tgcaccagct ggcgcgaggt tcgaatcagc ttgttgatcg
     2041 tttcgatcat gtgcaccggg atgcggatcg tcctcgcctg atccgcaatg gcgcgcgtga
     2101 tcgcctgccg gatccaccat gtcgcatagg tcgagaattt gtaaccgcgg cggtactcaa
     2161 atttgtccac cgccttcatc aggccgatat ttccctcctg aatcaggtcc agaaactgca
     2221 gtccgcgatt cgtgtatttc ttcgcgatcg acaccaccag gcgcaggttg gcttcaatca
     2281 gctcccgctt cgcctgctcg gcgtccatat cgccctggat catctcgcgc tgcgtccgct
     2341 tcagctccgc gatcgtgacg cccgcttctt cttccagctt ttccaggtcc gtcttgcagt
     2401 tcttctgctg gcgccggtac tccttcttca gctcttcgct gcggctctgc tcaaacttcg
     2461 tgtccaggga gcggatctgc cgttccagcg tccgcatcgc ctcgaccgtc ttgttcactt
     2521 tgtccagcag gcgcttgcgc tcgaggttgg tgtacttcag ctcgcgcaca atccgcgata
     2581 ccagcacctt ctcgcgcgcg atgctccagc gcagccggcg atgctccttc ggcttggctt
     2641 tctggttcac gccggccagc ttctcttcca gctgctgcgc ctttttctga tgcttcacca
     2701 gcgtgtccac gcgcgtcacc gtcgcgcgca cccgcgtctg caccacttct tcggtcagct
     2761 cctcatcgtc gaagatgacc acttccttga tgttgcgcac gccgcgcttc aggtcttcgc
     2821 ccagcgccac aatttcgcgg atcaccaccg gcgaccgcga aatcgccttc agcacgcgca
     2881 gctgtccgcg ttcgatccgc cgcgcaatct ccacttcgcc ttcgcgcgtc aacagcggta
     2941 ccgtgcccat ctcgcgcagg tacatgcgga ccggatcgtt tgtcttttcg agcgtccccg
     3001 gcgtcaggtc cagctcaacg tcttcgcctt cctcgccttc gaaatcgtcg cgctctccgg
     3061 gggtcccgat cttcggaccg ccttcgagta tgtcaatgcc ctgtgtgcca atggttgtga
     3121 tcagatcgtc gagatcgtcg ggcgagttca tgtcgcccgg aagaaggtcg ttgacctcgt
     3181 tgtaggtcag gtagcccttc tccttcccgg catcgatcag cttctgaata tcggcttcgt
     3241 atttatcttc aatcgccaag cggattggtc ccccgcgtga atttttggat ctgctgtctc
     3301 acctggagtg gacgaggtgc ggctagggcc atcaatggca taacccatag cggctcaatg
     3361 gccgctggga atagggcgca cgtctccccg ggaggtccag aataccacga aaccggccta
     3421 actccggcca ttgctgctat ttcgggcctg aacggcagtt cggtctcgcg tcgtcgggac
     3481 aggatcctca atcatttaga tgtggaaaac ccaaaattgt ttcatcacca gaccaggaag
     3541 agccaaacca ccgtaccgtg acccccgccc accggtaccg tgacccgccc agacagtacc
     3601 gttacccctg cgcacgggga ttcataagct gctgatcgtc tttcgccagc cgcttcttgc
     3661 cagcgcttca cccgcggacc ggttcttagc taaccgcttt tcagcttccc cccgctgtac
     3721 cctaaatccg aaccccgtgg ccaacgactt cgcccaatcc gtgaaagagc agtccgacat
     3781 cgtcaagatc atcggcgact acgtgcgcct caggaaggcc ggagcccaga actacaccgg
     3841 cctctgcccc ttccacaagg aaaagacccc atccttctcc gtccacgccg gccgccagtt
     3901 cttcaactgc ttcggttgtc acgagcacgg cgacgtcttc accttcatcc agaaaatcga
     3961 gaacgtcggc ttcttcgagg ccgtccgcac ggtcgcccag aagtccggca tcccgctccc
     4021 caaacgcgaa ttctcctcgc ctgaagaagc cgccgagcat cgccagcgcg ctaagctcct
     4081 cgacctgcac gaagccgcca ccgcctggtt tgaagagcag ctccgcgcgc ccgaagccgc
     4141 caccgcgcgc gaatacctca gcggccgcgg cctcaccgcc gacggcatcg ccaaattccg
     4201 catcggctac gcgcccgatt ccttccgcgc cctcggcgaa cgcctgcagt ccctcgccga
     4261 tcccgacacc ctccgccagt ccggcctctt ctcctggaag gaacaggaag acggcagtcc
     4321 cggccctatc tactcccgct tccgtaagcg catcatgttc cccatcgcca acgaagccgg
     4381 ccgcgtcatc gccttcaccg cccgcaccct cgaaaccggc gagaaggcgg gccccaaata
     4441 cctcaactcg ccggaaacgc cgctctactc caagagccac gtcctcttca atctcgataa
     4501 ggcccgccag gccatccgcc aggacggcgg cgccatcctc gtcgaaggcc aaatggactg
     4561 catctccgtc ttcctcgccg gcatccccaa cgtcatcgcc accagcggca ccgccttcac
     4621 cgaaccccag gtccgcctgc ttcgccgcta cgccacccgc gtcatcgtca acttcgatcc
     4681 cgacaccgcc ggcgccaacg ccgccgagaa atccatcgcc ctgctcgtcg aagagggctt
     4741 cgacatccgt gtgctcacgc tcgaagccgg cctcgacccc gaccgctaca tccgcga
//
LOCUS       DASZYN010000211         2041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_72177, whole genome shotgun sequence.
ACCESSION   DASZYN010000211 DASZYN010000000
VERSION     DASZYN010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72177"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VHE33_05445"
     CDS             <1..405
                     /locus_tag="VHE33_05445"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HVT96932.1"
                     /translation="AFAFVEPFFFIGYLISIAVFGLYQAIFMANAGGAWDNAKKVVEV
                     DLKQKGTPLHDATVVGDTVGDPFKDTSSVAMNPVIKFTTLFGLLAVELAVTLSHENPH
                     LAIGLAIAFFVVSMFFVRKSFYGMRIGSDAGK"
     gene            548..1450
                     /gene="ribH"
                     /locus_tag="VHE33_05450"
     CDS             548..1450
                     /gene="ribH"
                     /locus_tag="VHE33_05450"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HVT96933.1"
                     /translation="MIKGLTFIHPVHSAAHFDELVSFFSALGFEPGRGWDESPRKEGA
                     ASKGRPFLAPLGNLEFIDGTLPTLTTDLLVEVTSLDAVHQVAGHWLETHAGADAAKTR
                     ITSIENTHWKSRLFTVQPVPGFSVAFWECQEPVAGKPIALEGDLSAHEMSFAIVVARW
                     NAVITDRLLQGALDCLLRSGAHRDRIEVARVPGSWEIPSAARQLAQTHRFDAIITLGC
                     LIRGETAHYEAIYNEVARGIGQSQQDTGVPHAFGVLTCETLEQALDRAGLKSGNKGFE
                     AAAAAIEMASLHTKIVATPGEPRA"
     gene            1447..1863
                     /gene="nusB"
                     /locus_tag="VHE33_05455"
     CDS             1447..1863
                     /gene="nusB"
                     /locus_tag="VHE33_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HVT96934.1"
                     /translation="MTLVGKRRKSRELAMQMLFQADVGRQTPDEVRKTFWHAREEVDE
                     SARGFAEDLFRVATARATEIDAILEQYSKNWRIQRMSAVDRNLLRMAVAELLGFPGTP
                     APIVIDEALEIARRYSAPESIDFLNGMLDTIARSRL"
BASE COUNT          369 a          663 c          617 g          392 t
ORIGIN      
        1 gcgttcgcgt tcgttgagcc gttcttcttt atcggctacc tgatttcgat tgcggtcttc
       61 ggcttgtacc aggccatctt catggcgaat gccggcggcg catgggacaa cgcgaagaaa
      121 gtcgtcgagg tcgatctgaa acaaaaaggg acgccgctgc acgatgcaac cgtggtggga
      181 gacacggtgg gcgatccgtt caaagacacc tcgtcggtgg cgatgaatcc ggtcatcaag
      241 ttcaccaccc tgtttggact gctggcagtc gagcttgcag tgacactcag tcacgaaaat
      301 ccgcacctcg ccattggcct ggcgattgcc ttcttcgtgg tatcgatgtt cttcgttcgg
      361 aaatcgttct atgggatgag gatcggctcc gacgcaggga aatgacctct gttcgtggat
      421 cgaaaaagac gccgcctggg aacgggcggc gtttttgttc gcgcgcgatt ccacccaaac
      481 cgcaccactc gctgcggcca atccctgtga actgcggcct gtgctctgtc cttgataccc
      541 tcaaatcatg atcaagggcc tcactttcat tcacccggta cactcggcgg ctcattttga
      601 tgagctggtg agcttctttt ccgcgctggg ttttgagccg ggacgcggct gggacgagtc
      661 tccccgcaag gagggcgcag ccagcaaagg ccgacccttt ctcgcgcctc tgggcaatct
      721 cgagttcatc gacggcacgc ttcccacgct caccaccgac ctgctggttg aagtcacttc
      781 gctcgacgcc gtccaccagg tggccggcca ctggctcgag acgcacgccg gcgccgacgc
      841 cgcaaagacc cgcatcacct ccatcgaaaa cacgcactgg aagtcgcgcc tcttcaccgt
      901 gcagccggtc ccgggcttct ccgtcgcatt ttgggagtgc caggaaccgg tcgcgggcaa
      961 gcccattgcg cttgagggcg atctcagcgc gcacgaaatg agcttcgcga ttgtcgtggc
     1021 gcgctggaac gcggtcatca ccgaccgtct gctgcagggc gcgctcgatt gcctgctgcg
     1081 cagcggcgcg caccgcgatc gcatcgaggt cgcgcgcgta ccgggctcct gggaaattcc
     1141 ctccgccgct cgccagctgg cccagaccca tcgcttcgat gcgatcatca ctctcggttg
     1201 cctcatccgt ggcgaaaccg cgcactacga agccatctac aacgaggtcg cgcgcggtat
     1261 cggccagtcg cagcaggaca ccggcgtccc gcacgccttc ggcgtcctca cctgcgagac
     1321 gctggaacag gcactcgatc gcgccggcct caaaagcggc aataagggat tcgaggcggc
     1381 agcggcggcc atcgagatgg cctcgctgca cacaaagatc gtcgcaacac ctggggagcc
     1441 gcgagcatga cgctggtcgg caagcgccgc aaatcccgtg agctcgcgat gcagatgctc
     1501 tttcaggccg acgtgggccg ccagactccg gacgaggtgc gcaaaacctt ctggcatgcg
     1561 cgggaagagg tcgatgagag cgcgcggggt ttcgccgaag acctgtttcg ggtggccacg
     1621 gcgcgcgcca ccgaaatcga cgcgattctc gagcagtatt cgaagaactg gcgcatccag
     1681 cgcatgtccg cggtcgatcg caatctgttg cgcatggcag tggccgagct gctcgggttt
     1741 ccgggcaccc cggcgcccat cgtgatcgac gaagcgctgg aaatcgctcg ccgctattcg
     1801 gcgcctgaat ccatcgactt tctcaacggc atgctcgaca ccatcgccag gagccgcctg
     1861 tgaagagctg ggcgggggaa ggccgcagga gtgtcctgaa cccatgaagc tcttcgcctg
     1921 gctgctggaa gtcttcatca acaccttcgg catcacgcgc ccgcgccccg agcaggagcg
     1981 caccgctcag ctggtgattg gcggcttcct gctggctttc atcctcttcg cgatcgccgt
     2041 c
//
LOCUS       DASZYN010000212         8641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_843525, whole genome shotgun sequence.
ACCESSION   DASZYN010000212 DASZYN010000000
VERSION     DASZYN010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8641
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843525"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VHE33_05460"
     CDS             complement(<1..735)
                     /locus_tag="VHE33_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVT96935.1"
                     /translation="MILIATSFCAVLLFTFAVVSFATAPTKVDKTLQARLAAVTADKV
                     AGVSTASLEASELLRKTASSRWDWIGTSLSKYVSMASLQKWISQAALDTTASALLVQT
                     AAAAAIGFLITMFFAPILGVELAAAGGCATLPWARVAFLRYRRLSAFDKALPHAIDMM
                     ARSLRAGHSTAAAIEIMAQGAPEPAGSEFAEVFRQQNFGLPMRDALMQLIDRMPSPDL
                     RVLVTAIVVQRETGGNLVEVLDRTVHV"
     gene            complement(749..2131)
                     /locus_tag="VHE33_05465"
     CDS             complement(749..2131)
                     /locus_tag="VHE33_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HVT96936.1"
                     /translation="MSDLTLNLTQSRPDANTLRTNGPGLRSTTPGARPLREKEQQELK
                     SAVHAELINRLDLEKLGEAQETRAGQQQLFALIQQIIGEQGVPLSSSERDRVAQEVLD
                     EVFGLGPLEQLLKDDTISDILVNTFNAVYVERRGQLSRTSVSFRDNRHLLQVIDKIVS
                     AVGRRVDESTPMVDARLKDGSRVNAIIPPLAIDGPILSIRRFGSNPLTSEKLVQNKAL
                     TNPMLDMLRAAVQARMNIVIAGGTGSGKTTLLNALSSFISEKERIVTIEDSAELQLRQ
                     DHVVRLETRPPNVEGKGAIRQRELVINALRMRPDRIVLGECRGEEAMDMLQAMNTGHD
                     GSLTTVHANTPRDALSRLETMCMMGDIRLPEKAIRLQIASAIHIIVQAARMSDGTRRI
                     THVSELTGAYSDVISMQDIFVFEKEGVSDTGKVKGRFRSTGIVPKFTERLKAAGIPIP
                     AGLVDHSMEV"
     gene            complement(2134..3369)
                     /locus_tag="VHE33_05470"
     CDS             complement(2134..3369)
                     /locus_tag="VHE33_05470"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVT96937.1"
                     /translation="MSQTASNSNLSVALLTVGLERELTDKIVQSASQHRWSLSHDDFD
                     EYVSSVRRPAISADAKNADLVIAIIDFDRDAQGAAETASYLQHLFFGKFFAAALSSQT
                     EPELLLAAMRAGCNEVLRNPLDSRELGDLFDRQERQWSANTGRTRSTGHIISFFGAKG
                     GVGTTTVAVTLAYYLVRQKNKKVLLIDNHPEFGHVCLYLGLDGTRYHFDELIRNVNRL
                     DSTLLRGFIARHSSGLEVLSSPETHDLSRMVDPDALERTLEFLRGEYDYILLDCETSL
                     EEVSLAVMDRSDQIYIVATPDIGAIRDLSRYIDGLSRNESTSARLHVIINRYSSHGAI
                     AVEQIEKAIRVPVEVRLCNAYTECQRAINVGEPIGPDKKSEISTQFSRWTSILTGDET
                     PKAQPAKKGFALFGQKRTE"
     gene            complement(3390..4871)
                     /locus_tag="VHE33_05475"
     CDS             complement(3390..4871)
                     /locus_tag="VHE33_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HVT96938.1"
                     /translation="MLTIRSFSPALLVAALSAACSGAGAQNHALAVAAPAPFVGDMST
                     SAQVVGEPLHLLVGRSMFVNTDNRIRRIYVSNPDVLDSFTSSPRQIVITAKTPGVASV
                     VLWDEGGASHSYLVNSDLDVTGLNKAIKDAFPSENIRAEGHGAEVTLAGSVNSTATSD
                     AAFKLASLYSKDVANSLVVATPHIPQVKLKVRVVEIDRSRAMQLGFNFFGNNGSHTFN
                     TTTSQFGSVTAANSTTTTSTAGSTTQSAGASVSSLLELSNLLNLFYYNKSLGIGAAMQ
                     ALANKQILQILAEPTITTVSGEKASFLAGGSFPYPLIEPGLGGVNTVTVVFRDYGVKV
                     DFTPTVNADGTINMKIVPEVSALDYSNAVVLGGYTLPAISTRRADTRVELHSGQSFVI
                     SGLLDNRTIDQLEKVPGIGDIPILGKLFQSRSTNHSVTELAIIVTPTVIDPLAQDPAA
                     TVTQPKMVVPFINNPKFDKEIVPKTPPPQPQPNPRPEDVPVHD"
     gene            complement(4892..5710)
                     /gene="cpaB"
                     /locus_tag="VHE33_05480"
     CDS             complement(4892..5710)
                     /gene="cpaB"
                     /locus_tag="VHE33_05480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HVT96939.1"
                     /translation="MARRMMLALVVALVISGLFTFWLSRRVAHASRTVVQPPNQYVAA
                     ARALDPGEILRREDVNMINWPKDHPLDGSFVKVDDVVGRAVLYALSPGQPIVDRQLAP
                     KGSGLGLTGRIPSGMRAIALRSDEVVGVAGFLMPGTHVDVIVTYTDQEHPQPVTATVL
                     QDVEVLAAGHQVQPDPSGKPISVDVVTLLLAPEAAEKATLASEKGTIHFVLRNGGDHK
                     QSSAPPVNLAQLANLPTTKPEQGEPRPRAASHPAPKPWVVETMMGDKRSTASFY"
     gene            complement(5717..6259)
                     /locus_tag="VHE33_05485"
     CDS             complement(5717..6259)
                     /locus_tag="VHE33_05485"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HVT96940.1"
                     /translation="MANHLTEWLCPAVALGCALAGSVFDVRSRRIPNFITLPGILLGL
                     LLHLVLGGWRQMALAALAGLICGVLFLIFWLSGGMGAGDVKLITAVAAIAGMPLVPWL
                     LILTALAGGVMAVGLAVWRGRLRETVRNVGAVALHHRFEGLTPHPQLNLANARTLRLP
                     YALAVAAGTATTLFLTAFQR"
     gene            complement(6762..6923)
                     /locus_tag="VHE33_05490"
     CDS             complement(6762..6923)
                     /locus_tag="VHE33_05490"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HVT96941.1"
                     /translation="MKQLFKNLMNDESGQDLIEYALIAALIALGAIVAMGSLSTKIAT
                     EFNTIGNSL"
     gene            complement(7160..7330)
                     /locus_tag="VHE33_05495"
     CDS             complement(7160..7330)
                     /locus_tag="VHE33_05495"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HVT96942.1"
                     /translation="MDRSLTLLKSLLHDESGQDLIEYALIAALIAVAAIAAMRGLSTK
                     IATEFNTIGNSL"
     gene            complement(7552..7716)
                     /locus_tag="VHE33_05500"
     CDS             complement(7552..7716)
                     /locus_tag="VHE33_05500"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HVT96943.1"
                     /translation="MTRTTFETLLNDETAAGLIEYVLLAALIALAAVFAMHNFGKKLT
                     KDYKQIAKKI"
     gene            8261..>8641
                     /locus_tag="VHE33_05505"
     CDS             8261..>8641
                     /locus_tag="VHE33_05505"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HVT96944.1"
                     /translation="MTTIRNEDTGFRARLQRSIQLAGKRASARLRPCGQGEEGSTIAE
                     MALVLPVLLAVLTGVFSFGIALNQYLVLTNAVNNAARAFAMSAQNDASPSMLGSGDPC
                     KYAAQSIHDSATNMNTANITYTITY"
BASE COUNT         1694 a         2381 c         2875 g         1691 t
ORIGIN      
        1 gacgtggacg gtgcggtcga ggacttcgac caggttgccg ccggtctcac gctgcaccac
       61 gatggctgta accagaacac gaaggtcggg cgacggcatg cgatcgatga gctgcatgag
      121 ggcgtcgcgc atgggaaggc caaagttctg ctggcggaag acctctgcaa attcggaacc
      181 ggccggctcc ggcgcgccct gcgccatgat ttcgatggct gcggcggtgg agtgcccggc
      241 acgaagagag cgggccatca tatcgatggc atggggcaga gccttgtcga acgcgctgag
      301 gcggcgatag cggagaaagg caacgcgcgc ccagggcaat gtggcgcacc ctccggcagc
      361 ggctaactcg accccaagga tgggtgcaaa aaacatggtg atgaggaagc cgatggcagc
      421 agcggccgca gtctgcacca gcagcgcgga ggcggtggta tcaagggccg cctgcgagat
      481 ccacttctgc agcgaggcca tcgacacata tttgctgagg gacgtgccga tccagtccca
      541 gcggctgctg gcggtcttgc gcagcagttc gctggcttcg agcgaagcag tcgagacccc
      601 ggccaccttg tcggccgtga cggcggccag acgagcctga agcgtcttgt cgaccttcgt
      661 gggagccgtg gcgaagctga ccaccgcgaa ggtgaacagc aggacggcgc agaagctggt
      721 tgcgatgaga atcatgaccg atggcccctc acacttccat cgaatggtcg acgaggccgg
      781 cgggaatcgg aatgcccgct gccttcaggc gttcggtaaa tttgggaacg atgccggtgg
      841 aacggaagcg tcctttgact ttgcccgtgt cgctgacgcc ttctttttcg aagacgaaga
      901 tgtcctgcat gctgatgacg tcactgtagg cgccggtcag ctcggagacg tgcgtgatac
      961 ggcgggtacc gtcgctcata cgcgccgcct gcacgatgat gtggatggcg gaggcgatct
     1021 gaagacgaat ggctttctcg ggcagacgga tgtcgcccat catgcacatg gtttccagac
     1081 gcgacagcgc gtctcgcggt gtattggcgt gaaccgtggt gagcgatccg tcgtggccgg
     1141 tattcatggc ctgcagcatg tccatcgcct cttcgccgcg gcactcaccg aggacgatcc
     1201 gatcgggacg catacggagg gcgttgatga ccagctcgcg ctgccggatc gcgcccttgc
     1261 cttcaacgtt cggcggacgc gtctcgagcc ggacgacgtg atcctggcgc agctgcagtt
     1321 cggccgagtc ttcaatcgtg acgatgcgct ctttctcaga aatgaagctg gagagagcgt
     1381 tcagcagtgt ggtcttgccg gagcccgtac caccggcgat gacaatgttc atccgtgcct
     1441 gaacggcggc gcgcagcata tccagcatcg ggttggtgag cgctttgttc tggaccagct
     1501 tctccgacgt cagcgggttg gaaccgaaac ggcggatgga gaggatgggg ccgtcaatgg
     1561 ccagcggggg aatgatggcg ttgacgcggg agccgtcctt cagacgggcg tcgaccatcg
     1621 gcgtcgattc gtcaacccgg cgtccgacag cggaaacgat cttgtcgatg acctggagca
     1681 gatggcgatt gtcgcggaac gaaacgctgg tgcggctcag ctggccgcgc cgttccacat
     1741 agacggcgtt gaacgtattg accaggatgt cgctgatggt gtcgtccttc agcaactgct
     1801 cgaggggacc gagcccaaag acctcgtcga ggacttcctg agcaacgcga tcacgctcgg
     1861 aggagctgag cggaacaccc tgctcgccga tgatctgctg gatcagggca aagagctgct
     1921 gctggccggc ccgggtttcc tgggcttcgc ccagtttctc cagatccagg cggttgatca
     1981 gctcggcgtg gaccgcagac ttgagttcct gctgttcttt ttcccgcagg ggacgggcgc
     2041 cgggcgtggt gctgcggagt ccggggccgt tggtacggag agtattcgca tcaggacgtg
     2101 actgcgtgag attgagcgtg agatcgctca tcgctattca gtccttttct gaccaaacaa
     2161 ggcaaaccct tttttggccg gttgggcttt cggggtctcg tctccagtga gaatggaggt
     2221 ccagcgggag aactgcgtgg agatctccga cttcttgtcg ggcccaatcg gctcgccaac
     2281 gttaatcgct cgctggcatt cggtgtaggc attgcagagc cggacttcga ccgggacgcg
     2341 gatggccttc tcgatctgtt cgaccgcgat ggcgccgtgg gaggagtagc ggttgatgat
     2401 gacgtgcagg cgtgcggaag tggattcgtt gcggctgagg ccgtcaatat aacgggagag
     2461 gtcgcggata gcgccgatgt cgggcgtggc aacgatatag atctgatcgg atcgatccat
     2521 gaccgcgagg gaaacttctt caaggctggt ttcgcagtcc agcaggatgt agtcgtattc
     2581 gccgcgcaga aattccagtg tgcgctccag agcgtcggga tcgaccatgc gcgacaggtc
     2641 atgggtttcc ggagacgaga gcacctccag accactggaa tgccgggcga taaagccgcg
     2701 cagcagcgtg gaatcaaggc ggttgacgtt gcgaatcagc tcgtcgaagt gataacgcgt
     2761 gccatcgagg ccgaggtaca ggcaaacatg gccaaactcg gggtggttat cgatgagcag
     2821 gactttcttg ttcttctgcc ggacgaggta gtaagcgagg gtgacggcga cggtggtcgt
     2881 acccacgccg cctttggctc caaagaaaga gatgatgtgc ccggtggaac gcgtgcgccc
     2941 ggtattggct gaccactggc gctcctggcg atcgaaaagg tcgcccagct cgcggctgtc
     3001 gaggggattg cggagaacct cattgcagcc ggcacgcatc gcggccagca gcagctccgg
     3061 ttctgtctgg ctcgacaggg cggctgcaaa gaatttcccg aagaagagat gttgcaggta
     3121 gctggcggtt tctgcagcgc cctgggcatc gcggtcgaaa tcgatgatcg cgatgacaag
     3181 gtcggcgttt ttcgcatcgg cggagattgc gggacggcga acggaagaaa cgtattcgtc
     3241 gaaatcgtcg tgcgataagg accagcggtg ctgggaggcg gactgaacga tcttatcggt
     3301 cagttcgcgc tccagaccaa cggtcagcag agcaaccgag aggttcgaat tagatgccgt
     3361 ctggctcatc tgggtgagtg tcctttttct tagtcgtgta ccggcacgtc ttcaggcctc
     3421 gggttcggct ggggctgcgg aggcggcgtc ttcggcacga tttctttgtc gaacttcgga
     3481 ttgttgatga acggaacgac catcttcggc tgcgttacgg tcgcggccgg atcctgggcg
     3541 agcggatcga tcaccgtggg cgtgacgata atggccagct cggtgacgga atggttggtg
     3601 gacctcgact ggaacagctt gccgaggatc ggaatatcgc cgatgccagg aaccttttcc
     3661 agctgatcga tggtgcggtt gtcgagcagg ccggagatca cgaagctctg tccgctgtgc
     3721 agttccacgc gcgtgtcggc gcggcgggtg gaaattgccg gcagcgtgta accgccgagg
     3781 acaacggcgt tggaatagtc gagcgcactg acctcgggaa cgatcttcat gttgatcgtg
     3841 ccgtccgcgt tgaccgtcgg ggtaaagtca acctttacgc cgtagtcgcg gaacacgacc
     3901 gtgacggtat tgacgccgcc cagacccggt tcgatcagcg gatagggaaa cgatccgccg
     3961 gcgaggaacg atgccttctc gccgctgacg gtggtaatcg tgggttcggc gaggatctgg
     4021 aggatctgtt tgttcgccag tgcctgcatc gcggcgccga tgccgaggct cttgttgtag
     4081 tagaagaggt tgagcaggtt gctcagctcg agcagggaag agaccgaagc cccggcggat
     4141 tgggtggtgc tgccggctgt ggacgtggtg gtggtgctgt tggcggcggt cacggagccg
     4201 aactgcgacg tcgtggtgtt aaaggtgtgg gagccgttgt tgccaaagaa gttgaaccca
     4261 agctgcatgg cgcgggaacg atcgatttcg accacgcgca ccttgagctt cacctgggga
     4321 atgtggggcg tcgccacgac cagtgaattc gcgacatcct ttgaatacag gctggcgagt
     4381 ttgaaggcag catcgctggt ggcggtgctg ttcacggacc cggcgagagt gacttcggcg
     4441 ccgtgaccct ccgccctgat gttttccgac gggaacgcat ctttgatggc cttgttcaga
     4501 ccggtcacgt cgagatcgga attgaccaga tacgagtgcg aggctccgcc ctcgtcccag
     4561 agcacgacgc tggctactcc cggggttttg gccgtgatca caatctgacg cgggctggag
     4621 gtgaaggaat ccagcacatc cgggttgctg acatagatcc ggcgaatgcg attgtcggtg
     4681 ttgacgaaca tggagcggcc gaccagcagg tggagaggtt cgccaaccac ctgagcgctg
     4741 gtgctcatgt ctccgacaaa cggggcagga gcagcaaccg caagggcatg gttctgggct
     4801 ccggcgccgg aacaggcggc ggacagtgca gccacgagca gagccgggga aaaagatcga
     4861 attgtgagca ttctgcgaat catggttggc ctcagtagaa actcgccgtg gagcgcttat
     4921 cgcccatcat ggtttcgaca acccagggtt tcggagccgg atgactggca gctctgggcc
     4981 gcggctcacc ctgttccggt ttggtggttg gcaggttcgc cagttgcgcc aggttgaccg
     5041 gaggagcgct ggactgtttg tggtccccgc cattacgcag cacgaagtga atcgtgcctt
     5101 tctcgctggc cagagtggct ttttcagcag cttcgggggc cagcagcagg gtgacgacat
     5161 cgacggaaat cggtttcccg gagggatcag gctgaacctg atggccggcg gcgaggactt
     5221 cgacatcctg aagcacggtc gcggtgaccg gctgcggatg ctcctggtcg gtgtaggtga
     5281 cgatgacgtc cacatgcgtg ccgggcatca ggaagccggc aacgccgacc acttcgtccg
     5341 aacgcagcgc gatggcgcgc atgccgctgg gaattcgtcc cgtcagaccg agacccgatc
     5401 cttttggagc gagctgacgg tcaacgatcg gctggccggg ggacagggca tacagaacgg
     5461 ctcggccgac gacgtcgtca accttcacaa agctgccgtc cagaggatgg tcctttggcc
     5521 agttgatcat gttgacgtct tcgcggcgaa ggatttcgcc gggatcgagg gcgcgggcag
     5581 cagccacata ctggttcggc ggctggacaa ccgtgcggga ggcatgtgca acccggcggc
     5641 tgagccagaa cgtaaagagg ccggagatca ccagggctac gacaagggca agcatcatgc
     5701 ggcgggccat atcaggtcac ctttggaacg ccgtgaggaa cagcgtcgtc gccgtgcccg
     5761 cggcaacagc cagggcatag ggaagacgca gtgtcctcgc attcgcgaga ttgagttgag
     5821 ggtgaggggt aagaccttcg aaacgatggt gcagagcaac agctcccacg tttcgaacgg
     5881 tttcccggag acggccgcgc cacactgcga gaccgacagc catcacaccg ccggcgagcg
     5941 cggtgaggat cagcagccag gggaccagag gcatcccggc aatggctgcg accgcggtga
     6001 tcagcttcac gtcaccggcg cccatcccgc ctgacagcca gaaaatgagg aagagcacac
     6061 cgcagatgag cccggcgagg gcggcgagag ccatttgtct ccagccaccg agaaccaggt
     6121 gcagcaggag accgagaagg attccgggca gggtgatgaa gtttggaatc cggcggctgc
     6181 ggacgtcaaa gacagatccg gccagagcac aacccagcgc gacagccgga cacagccact
     6241 cggtaagatg gttcgccatg cgatgacccc agatgcgctg tccgcctgat cggcgagcag
     6301 agcaggaact tgatccgcgc cccgaaaccc ggtgaggcta tccggtgcgg cggatgcaga
     6361 tcgtggaagg agcgagcgat gtggagggaa gaacgtcgtg gggcactagc tccgcggtcg
     6421 ttagccggca catcgcgccg cggcatcccg gcctggttcc gcgagggaag ttcgccagga
     6481 aatccgcgcg atgctgcgtc gtccttcggt tcgtttccgg gcgtgcaggc cgttgttgca
     6541 tgcctgagcc gggaaagggt tggagatgaa caaccagacc agcgtcatta cggttcggaa
     6601 tcttgccgca gaggtcgggt ggtcgcgacc ggaggcgggt gagcctggtc gcgaccggat
     6661 cctgcagagc ggtcaggccg taggagcttg ggagagcggg cctgcgccgt accaccaacc
     6721 agagaccggc aaaacaaaaa ccgtcaggac atcagtgttg gttacaggct gttgccgatg
     6781 gtgttgaact cagttgcgat cttggtgctg agggagccca tcgcgacgat ggcgcccaga
     6841 gcgatgaggg ccgcgatcag cgcgtactcg atcaggtcct gaccggactc atcgttcatc
     6901 aggttcttga aaagctgttt cattaccgtt ctccttgtgg tgtggcggcg aggctttcca
     6961 tgctggaccc cgtaccgcgc tgtgttgatg gtgattgttg tcacattgct ccggataacc
     7021 gctccatcgc aatgccattt aagtgttacc ctttgctact ttggtcaaaa actatggtga
     7081 tgagcttcgt taacttcaag ggtttcaagt actacatcgg acggtactgc ggttttgctg
     7141 agcaccgtct gcagggacgt cagaggctgt tgccgatggt attgaactca gttgcaattt
     7201 tggtgctcag acccctcatg gcggcgatcg ccgcaaccgc aatcagagct gcgatcaggg
     7261 cgtattcgat cagatcctgc ccggattcgt cgtgcagcaa acttttcagg agcgttaaag
     7321 accggtccat ccttccccct tgcttcgcag ggtctggcgc cctgcggcga cgagcctgct
     7381 gttttgcaat cgttgttgtc atcttgcgat ggagtacccg cgccagcaat gctcaggaag
     7441 tgttaccaat tgctactttc ggtgttacgg tcgctccaaa tgtggacgcc tggcggggaa
     7501 cggggcatgg aaccagggtc gtacacgtga atcaccgctg ccgccctgct ctcagatctt
     7561 tttggcgatc tgcttgtagt ccttcgtgag cttcttgccg aaattgtgca tcgcgaagac
     7621 cgccgccagt gcgatcaacg cggccagcag gacgtactcg atgaggccgg cggcggtttc
     7681 atcgttcagg agcgtttcga aagtggttcg agtcatgatc atgggttcct ctgtcgggcc
     7741 gggaatcgct gatgcccgtg cccggtggac ccggaggcgg agcagatcag ggcgtccacg
     7801 ctaatgaacc cgcagcagga aaatcatccc tcatcggtac cacgggtccg tccttctttt
     7861 ggggcagacc ggccatcgcc gcggagtgaa atcctgacac ttcccggagg gaacgaaggg
     7921 cctgcggcac tcgagccgta acgcaggggg gacagggaac tgcggtaact gcgattccat
     7981 tttggaaccg agcagctgcg aacaaagttc cgatgaagtg ttgcaaatcg atgcaaattg
     8041 ccctcggaat tactggggtg agtaatttgt gtgacttacc gagagggaat ttggtaccac
     8101 gaagtagcca ttgagtctcc tgctcgctgc actaatcttt agtgaagaaa attcccctgt
     8161 tcaatgcgcg ggagcggcgc acggtttcgc cagacgcggc taccggtgtg ccgggaacga
     8221 agctgatttt cttcaggagt tacgagcgcg gatgaacccc atgacaacga tccggaacga
     8281 ggacacggga tttcgagccc ggctgcagcg ctcgatccaa ctggccggga aaagagcctc
     8341 tgctcggctc cggccctgcg gccagggcga agaaggttcg accatcgccg aaatggcgct
     8401 ggtccttccc gtccttctgg cggttctcac cggcgtgttc tcgtttggca tcgcactgaa
     8461 tcagtacctg gtcctgacca atgccgtgaa caatgcggcg cgcgccttcg cgatgagcgc
     8521 ccagaacgat gccagcccct cgatgctggg cagcggcgat ccctgcaaat acgcggctca
     8581 gagcattcat gactccgcta cgaacatgaa taccgcgaac atcacttaca cgatcactta
     8641 c
//
LOCUS       DASZYN010000213        11404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_458526, whole genome shotgun sequence.
ACCESSION   DASZYN010000213 DASZYN010000000
VERSION     DASZYN010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 11404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11404
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458526"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..1122)
                     /locus_tag="VHE33_05510"
     CDS             complement(259..1122)
                     /locus_tag="VHE33_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96945.1"
                     /translation="MTNPTSTQESLVVGYFADGANAYSAINELIDEGFHASEIGAAFR
                     APRAASSPGAAAQEQSASEMKGVRELTERNPAVSGTVGGPASRDQAVQPAGLAPGSGN
                     AFPAPPSTPGPIPGSEIPSTLKHDLPETLPHELPSTLRPRSEADAPWLEQLHRNWSSS
                     TAQTAKNRNSAATGNQKFGTGEATLGLEQTWSEPAFESSFTGMGLSPEQARGLSGELG
                     RGGAVITINAGARSSLAEAIVERNHGRVRLEYSAGTTTLRPSDRESPVQIYGSMGNYY
                     RTDTEQKKKAS"
     gene            1272..1365
                     /locus_tag="VHE33_05515"
     tRNA            1272..1365
                     /locus_tag="VHE33_05515"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1306..1308,aa:Ser,seq:gct)
     gene            complement(1498..9549)
                     /locus_tag="VHE33_05520"
     CDS             complement(1498..9549)
                     /locus_tag="VHE33_05520"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HVT96946.1"
                     /translation="MAAVLGCALASSGFAQSIQTLQNNVRPEVSAGQAPLISAMPLGQ
                     QVNFSLVLPLRNQAQLNAFLTRLYDPSSPDFHKFLTVDQFTAQYGPTQQDYQAVLAYA
                     QANGFTVTGTPANRLVVPLSGAVSQIEAAFNVRMNVYQHPVENRTFFSLDREPSLNLS
                     VPVAHISGLDSYSLPQAMVIPPPPETALLASGSGSGPGGSYLGSDMRAAYYGGTTLDG
                     NGQAVALLEFDGYNLSDVNATFTNAGQTYNVPVNNVLLDGASGAPTSSGEAEVVLDIV
                     QAIGMAPGLSQVRVYISNGLDDANILNSIASENIAKQISCSWSWRPADPATDDPFFKE
                     FAAQGQSFFVASGDYGAFDATVSPFFYPQEDAYITAVGGTHLTTTSAGGPWSDETAWN
                     DFPQNFIGSGGGISPDSIAIPSWQSGVANTSNAGSSTLRNVPDVSMEADQDNYNCTYG
                     SCSSGWAGTSFAAPRWAGFMALVNQQAVEAGNAPLGGIGFLNPSLYILAQGSPYSTDF
                     HDITSGNNDTQNQSLSYNAVAGYDLVTGWGSPTGQHLIDDLAGPQVPGFWIIGSPATV
                     GVLQGASASTTVSVTDAGGFTGNVNLAVTTPLPTGVTATWGTNPTSASSVLTFTADSN
                     APASTTPLTITGTSGSLSVNTTVTLAVHAPTFLLTPSPSSLGLSPGSSGTSTITVTPQ
                     YGFTGAVSLSISGLPAGVTASFSPVSTSGTSTLTLNASNTAVDGTTNLIITGTSGNIT
                     VTKILPLTIHEPTFQLVTQVSVNLGQGTTGTAPVYIVPSYGFSGSVNLSVSGLPTGVT
                     ASFLPNPATASSTLSLTVGASVPVGTTPLTITGTSGNLTITTTISLVVHQPTFTVYSP
                     STMDMGQGTTYNTYISMTPQYGFTGIVTYSISGLPAGVTASWSQNPTSSTYTTLVLTS
                     SSSTAVGATKITITGTSGSLTSSSTTTLTIHQPSFTVYGPSSLDMGQGSSVNAYVSIN
                     SQYGFSGNVTFSVTGLPNGVTALWNPNPTTGSTNLTLNSSSSTAVGPSTITITGKSGT
                     ITASTTATLTIHAPSFTVNTYSSLSIGQGSTATSYVSINSAYGFNGSVNLSVSGLPTG
                     VTASFSQNPTTSSSSLTFTATSTAAIGSKTVTITGTSGALTATATLTLAVYAPTFTIS
                     GPGAADIGQGTTTAFTFYVNPQYGFASSVNFSISGLPSGVTASFAPNPTTNSTLLTLV
                     ASATAPAGQSTVTITGTAGSLTATATLTIGVHAPSFTISSGYSQNLGQGSSVTSTVWL
                     YPQYGFSGNVNLSVSGLPTGVTASFSPNPVAISGTSYSSSSILTLTAASTVTPGQYTL
                     TITGSSGSITTTTPFYLGIFAPAFTLSSGSIYMGQGTTSTTNVWAYPQYGFTGDVNLS
                     VSGLPSGVTASFAPNPVALTAGNYSAVSALTITAGSTVPPGQYNITITGTSGSQTTTT
                     TLYLAVYAPSFYVNCSCYNFSLNQGTSAQGTVTITPQYGFTGSVNLTISGLPNGVTAS
                     FSPNPTTSTSTLTMTAGTNAAPVNAAPITITGTSGSLTYTTTAYVTVNAQGFSLISAP
                     SQIKLARGSSANATLMVVPYNGFTGDVSFSASGLPAGVTAAFSPNPTTGSSSMTIAAD
                     SSAASGTVPVVVTGTSGSITATSSLILEVDATAPATTATTLEVSAAGSAANSVAAGTV
                     VTLTAAVNAGSTTVKSGQINFCNAVTNGCDATHLIGAAQLTSAGTAILKFIPGMGVHS
                     YKAVFSGTATNAGSASSASALTVTSSQPTTTALAAAGSAGNYTLTATVAGAGSVAPTG
                     TVSFIDATNNNSVLATADLAKTKTTITWGNPQAPATGQYPLAIATADFNQDGIPDLAI
                     ANNNAATVTILLGKGDGTFTAAASALSVGNYPTAIVSGDFNGDGIPDLAVANSNSNYI
                     SIFVGKGDGSFTPSSNNLPTGSQPVALVLGDLNGDGIPDLAALNASSGTVTVELGNGD
                     GTFTPSPLSAQAGSYPRAMSAADLNGDGILDLAVVGGYNSTLAILLGNGDGSFTAAIP
                     VTLPQSSNPYGVIAADFNQDGNPDLAVIDSYTNVIDIFLGHGDGTFAAAASNATGLNS
                     VSKSSAADLNGDGKPDLVEVNGSQMGILLGNGDGTFAAITTVSAGNYPSAIAAGDFNG
                     DGTQDLAITNTYNNTAVILTSQLSQTITAIATGVSPVGTGTHNVQASYPGDTSYKSSV
                     STTVALAAQLATPTVTITPSSVSITTGQPLTVTITVAGNGGNPTPTGSVVLTSGTYTS
                     ATATLASGGASITIPAGALATGSDTLSVHYTPDSASAAIFNPATGTVPVTVGKVTPTV
                     TVTPSALSITTLQPLSVTVAVNSGAGGPAPTGSITLTSGSYTSAATPLSNGSATINIP
                     AGSLSDGKDTLTATFAPDAAGASNWNTAVGSSSVTVFPAGGTTVATVAIQPTASTITS
                     LQSVTVTVTVSGPVGQPVPTGAVSLSSGTYSAQQPLVSGSASFTIAAGALASGADTLT
                     AAYSGDATYATASGSATVTVAPVLMAAPTLNSVLPGSTATGNVTFTAGSYSGTMSLTC
                     TLTASPSGAQSLPGCSLNPTSVTLASGGTATAAVTITTTAASTASLVPPAQFQLWKFG
                     GAGSLLAAVFMFGIPARRRRWASMLALLLFVAIAGAIGCGGGSGNSTQPPPAHTTPAT
                     TAGNYTFNVRATDSAANVTTSANVTLTVQ"
     gene            complement(10051..11001)
                     /locus_tag="VHE33_05525"
     CDS             complement(10051..11001)
                     /locus_tag="VHE33_05525"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT96947.1"
                     /translation="MRVSVAVLTALLAAGTACYSQASGWPPGPEHATITLWPHGAPTP
                     AVEPGAFPTPATPPGPEHDTTTAKEPTVAGRAVVRLGNVTVPTITLYLASKTNNTGAA
                     VVVFPGGGYRILAIDLEGTEVCDWLNSIGVNCLLVKYRVPDTGPYPKSSAALQDAQRA
                     LGIARAHASDWAIDPHRIGVLGFSAGAHLSAALSTHYDQRLYPHIDAADDQSCKPDFA
                     FIIYPGYLALADKNMAFNPDIPVTSATPATFLLQAEDDPVHVENVTQYFLALKKAAVP
                     AELHVYTKGGHGYGLRPTELPITHWPALAATWLHTIGVLH"
BASE COUNT         2159 a         2735 c         4235 g         2275 t
ORIGIN      
        1 ttcctgttga tgttttgagc gaaaaggatt ccgctgcttg ctgtggggtg aacctggccc
       61 tttcccagct gtggtctgtt tcttttcttc cggcctgtgc tcaggtgtga ggcagtttgt
      121 tttttcctgg cgtgtgcaca gctgtgatgt ggttcggttt ttccggaatg gtccccgggg
      181 ggcggggcga ccctcgtccg atcgaaagca aaacgccact ccgaagagtg gcgtttttgc
      241 ggaaggcgaa aaccgccttc aggaggcttt cttcttctgc tcggtatcgg tgcgatagta
      301 gttgcccatc gagccgtaaa tctggaccgg actttcgcgg tcggacgggc ggagcgtggt
      361 ggttccagcc gagtactcga ggcggacgcg gccatggttg cgttccacaa tggcttcggc
      421 aagagaggaa cgcgcgccag cgttgatggt gatgacggcg ccgccgcggc cgagttcgcc
      481 gctcaaaccg cgcgcctgct cggggctgag gcccatgccg gtgaacgagg attcgaaggc
      541 cggctcggac caggtctgtt cgagaccgag ggtggcttca ccagtaccga acttctgatt
      601 gccagtggca gcggaattgc ggttctttgc cgtttgcgcc gtgctgctgc tccagttgcg
      661 atggagctgt tcgagccagg gcgcgtcggc ctcgctgcgc gggcgcagag tgctgggcag
      721 ttcgtgcgga agcgtctcgg gcaaatcgtg tttcagggtg gaaggaatct cactgccggg
      781 aatggggccg ggggtggagg gtggagcggg aaatgcattg cccgatccgg gcgcgaggcc
      841 tgcgggttgt acagcctggt cgcgcgacgc ggggccgccg acggttccgg agacagcggg
      901 gttacgttcc gttaattcgc ggacaccttt catctcgctt gcggattgtt cctgtgctgc
      961 cgcccctggg ctggatgcgg cacgtggcgc gcgaaaggcg gcgccgattt ccgaggcgtg
     1021 gaatccctcg tcaatgagct cgttgatggc cgaataggcg tttgcgccat cggcgaagta
     1081 tccgacaacg aggctctcct gggtgctggt cgggttggtc attgtgctgc gtactcctgg
     1141 ggccgaatag gcacgagaat tgggatgcgg cggagcgcgt tcgcggtatc cagcggggaa
     1201 aagaaggggg tggaaggagg gatttcgggt ctgggacgga aggttccgcg tatactggcg
     1261 acagccgagc cggagagatg gccgagtggc tgaaggcggc ggtttgctaa accgttatag
     1321 ggttgaaaag ctctatcgag ggttcgaatc cctctctctc cgccagatta tctaacgaat
     1381 cccgactcca ggggccttgc gcaggatttt gcctgcggtc ttagtgaccc gcacatcggc
     1441 tcaaagtcaa attttctatc tttcttgccc gcatgacccg caccgtcggg cctttttcta
     1501 ctgcaccgtt agggtgacgt tggccgaggt tgtcacgttg gcggcggagt cggttgctct
     1561 cacgttgaac gtgtagttgc cggcggttgt cgcgggcgtg gtgtgggcgg gtggaggctg
     1621 agtgctgttg ccgcttccac caccgcaacc gattgctcct gcgatggcaa caaagaggag
     1681 cagcgcgagc attgaggccc agcggcgccg acgcgcgggg atgccgaaca tgaacacggc
     1741 ggcgaggagt gagccggctc caccgaattt ccagagctgg aattgtgcgg gcgggacgag
     1801 cgaggctgtt gaagcggccg tggttgtgat ggtgacggcc gcggtggcgg ttccacctga
     1861 ggcaagtgtg acgctggtgg ggttgagcga gcagccgggg agactctggg ctccggaggg
     1921 cgacgcggtg agcgtgcagg tgagactcat ggtgccggag tagctgccgg cggtgaaggt
     1981 gacgttgccg gtggcagtgg atccgggaag gacggagttg agggtaggtg cggccatcag
     2041 caccggagca acggtgacgg tggcagagcc gctggcagtg gcgtaagtgg cgtcgccgga
     2101 gtaggcggca gtgagcgtat cggcgccact ggccagcgcg ccagctgcga tggtgaagga
     2161 tgcagatccg ctgacaagag gttgttgggc ggagtaagta ccgctggaga ggctaactgc
     2221 gccggtggga acgggctggc cgacggggcc gctaactgtc actgtgacgg ttacactttg
     2281 cagactggtg atggtggaag ctgtgggctg gatcgcgacg gtggccacgg tcgttccgcc
     2341 ggcgggaaat acagtaactg agctggagcc gacggcggtg ttccagttcg aggcgccggc
     2401 ggcgtcaggt gcgaaggtgg cggtgagtgt gtccttgccg tcagacagag atccggcggg
     2461 gatgttgata gtcgcgctgc cgttgctgag cggcgtcgca gcggaggtat aactgccgct
     2521 ggtgagcgtg atggaaccgg tgggcgctgg gccacctgcg ccgctgttga cggcgacagt
     2581 gaccgagaga ggctgcaatg tagtgatgct gagtgcagag ggcgtgacgg tcaccgtggg
     2641 agtgaccttt ccaaccgtga cgggcacggt tccagttgcc gggttgaaga tcgcggcgct
     2701 agcgctgtcg ggggtgtagt ggacagacag ggtgtcgctg ccggtagcga gggctccggc
     2761 agggatggta atggaggcac cgccgctggc gagggttgct gttgccgatg tgtaggtgcc
     2821 gctggtgaga acgacagaac cagtcggagt gggattgccg ccgttgccgg cgacggtgat
     2881 ggtgacggtg aggggctgac ccgtggttat gcttaccgat gaaggtgtaa ttgtgaccgt
     2941 tggagtggct aactgggcgg cgagggcgac ggtggtggag acactggatt tgtaactggt
     3001 gtcgccggga taactggcct gaacgttatg ggtgccggtg ccgacgggag agacgccggt
     3061 ggcgatggcg gtgatggttt gggatagctg cgacgtcagg atgaccgcgg tgttgttata
     3121 ggtgttggtg atggcaagat cctgggtacc gtctccattg aaatcgccgg cggcgatcgc
     3181 agatggatag ttgccggccg ataccgtggt gatagcggcg aaggtgccgt cgccgttgcc
     3241 cagcaagatt cccatctgag agccgttgac ttccacgaga tccggcttgc cgtctccatt
     3301 gagatcggcg gcggaactct tactcacgga gttcagacct gtcgcattcg acgcagcggc
     3361 ggcgaaggtt ccgtcaccat gaccgaggaa aatgtcgatg acgttagtgt aactgtcgat
     3421 aacggcgagg tccggattgc catcctggtt gaagtcggca gcgatgactc cataggggtt
     3481 ggaactttgc ggcagcgtta caggaatggc cgcggtaaag gagccgtcac cgttcccgag
     3541 caggatggcg agtgtgctgt tgtatccacc gacgacagca agatcgagaa tgccgtcgcc
     3601 gttcaagtca gcggcggaca ttgctctagg ataggaaccg gcctgggcgc tgaggggaga
     3661 gggcgtgaat gtgccatcgc cgttgcctaa ctccaccgtg acggttccgc tgctggcgtt
     3721 gagagcagcc agatcgggga tgccgtcgcc attgagatcg ccgagaacca gggccaccgg
     3781 ctggctgccg gtgggcagat tgttagagga cggcgtaaag gagccatcgc ctttgccaac
     3841 aaagatgctg atgtaattcg aattgctgtt ggcaacggcg aggtcgggga tgccatcgcc
     3901 gttgaaatcg ccggagacaa tggcagttgg ataatttccg acagaaagcg cggaggcggc
     3961 cgcggtgaag gtgccatcgc ctttgccgag gaggatagtc acggttgcgg cattgttatt
     4021 agcgattgcg aggtccggga taccgtcctg gttgaaatcc gctgtggcga tggccagggg
     4081 atactggccg gtggcgggcg cctggggatt gccccaggtg atggtcgttt tggtcttggc
     4141 taagtcagct gtggcgagaa cggagttatt gttcgtggcg tcgatgaagg agacggtgcc
     4201 tgttggcgcg acggatccgg caccagcgac ggtggcggtc aaggtatagt ttcctgcgct
     4261 gccagcagcg gcaagggccg tggtggtggg ttgcgaggag gtgacggtga gcgcggaggc
     4321 acttgatgcg cttcctgcgt tggtggcggt gccggagaag accgctttgt aactgtgcac
     4381 acccatgccg gggatgaatt tcagtattgc ggtaccggca gacgtcaatt gagcggcgcc
     4441 aatcagatgg gtcgcgtcgc agccgttcgt gactgcgttg cagaaattga tttggcctga
     4501 tttgactgtg gtcgatccgg cgtttacggc tgcggtgaga gtcacaacgg ttcctgcggc
     4561 cacggagtta gccgcgctgc cggcggcgct gacctcgaga gttgttgccg tcgttgcagg
     4621 tgcggttgcg tcgacttcca ggatcaacga ggaggttgcg gtgatggagc cggaggttcc
     4681 ggtaacgacg acaggcacgg ttccggacgc ggcggagcta tcggctgcga tcgtcatcga
     4741 actggaaccg gtggtggggt taggagagaa ggcggctgtg acaccggcgg gcagcccgga
     4801 ggccgagaaa ctgacgtctc ccgtgaaccc gttgtacgga actaccatca gcgtcgcgtt
     4861 tgcggagcta cccctggcga gtttgatctg gctgggtgcg ctgatgagcg agaagccctg
     4921 agcgttgacg gtgacataag ccgtggttgt gtaggtgagg gagccggagg taccggtgat
     4981 ggtgatcggc gcggcgttga ccggcgcggc gttggtgccg gcggtcatcg tgagcgtaga
     5041 ggtcgaagtg gtggggttgg gagagaagga ggcggtgacg ccattgggaa ggccggagat
     5101 ggtgagattg acgcttccgg tgaatccata ctgcggagta atcgtgacgg taccctgcgc
     5161 cgatgtgccc tgattgaggc tgaagttgta acacgagcaa ttgacgtaga aactcggcgc
     5221 atagacagca agatagaggg tcgtggtggt ggtttgcgag ccagaggttc ctgtaatggt
     5281 tatgttgtat tgaccgggag gcacggtgct gccggcggtg atggtaagcg cgctgacagc
     5341 agaatagttt ccagcggtaa gagctaccgg gttgggcgca aaggaagccg tgacaccgct
     5401 gggcaggccg gagacggaga gattgacgtc gccggtgaat ccatattgcg gataggccca
     5461 gacattggtc gtgcttgtcg tgccctgccc catgtagata ctgccgctgg agagggtaaa
     5521 ggccggggcg aagataccaa ggtagaaggg cgtggtggtg gtgatcgagc ccgagctacc
     5581 tgtgatggta agcgtgtatt gaccgggagt gacggtgctg gcggcggtca gagtcaggat
     5641 gctggagctg ctgtaactcg ttccggaaat cgcaaccggg ttaggagaga aggaagccgt
     5701 gacaccggtg ggcagccctg agacagagag attcacattg ccgctgaaac catattgggg
     5761 gtagagccac accgtggatg tgacggagct gccctggccg agattctgac tgtagccgct
     5821 ggagatggtg aaggaaggcg cgtgaacacc aatcgtgagc gtggcggttg cggttaggga
     5881 gcccgctgtg ccggtaatgg tcacagtcga ttgccctgca ggcgcagtgg cgctggcgac
     5941 gagtgtcaac agcgtggagt ttgtagtggg attcggggcg aatgaagctg tgacgccgga
     6001 aggcagtccg gagatggaga agttcacgct gctggcaaat ccatattgcg gattcacgta
     6061 gaaggtgaag gccgtagtgg ttccctgacc gatgtcagcg gcacccggac ccgagatggt
     6121 gaaggtggga gcataaacgg caagggtgag cgtggcggtc gcggtgagtg cgccggaagt
     6181 tccggtgatg gtgacggtct tcgatccgat cgccgcagtg ctggtggcgg tgaaggtgag
     6241 gctgctcgaa ctggtggtgg gattctgtga aaacgacgca gtgacgccgg tgggcagacc
     6301 ggagacagag agattcacgc tgccattaaa gccgtatgcg gaattgatgg agacgtacga
     6361 agtcgccgtc gaaccctggc caatgcttaa cgaagagtag gtattgaccg tgaacgacgg
     6421 agcgtgaatg gtcagggtcg cggtggtgct ggccgtaatg gttccggatt tcccggtgat
     6481 ggtgatggtg ctcggtccga ccgcagtcga gctgctcgag ttcaatgtca ggttggtcga
     6541 tccggtggtt ggattcggat tccagagcgc ggtgacgccg ttgggcaggc ctgtaacgga
     6601 gaaggtgaca ttcccgctga atccatactg cgaattgatg ctgacgtagg catttacaga
     6661 acttccctgc cccatatcga gggacgacgg cccatagacc gtgaacgacg gctggtgaat
     6721 cgttagggtg gtggtagacg atgacgtgag gctgccggag gttccggtaa tagtgatctt
     6781 ggttgcaccg acggcagtcg aactgctgga ggtcagcacg agggtggtgt aagtcgacga
     6841 tgtggggttc tggctccaac tggcggtgac gccggcgggg agtcctgaga tcgagtaagt
     6901 gacaatcccg gtgaatccat attggggcgt catcgagata taggtgttgt aagtggttcc
     6961 ctgccccata tccatcgttg acgggctgta aacggtgaag gtgggctgat ggacgaccag
     7021 actgatggtg gtcgtgatgg tcaggttacc ggaggtgccg gtaatggtca gcggggtagt
     7081 gccgacgggt acggaggcac ctaccgtgag cgaaagagtg ctggacgctg tggccgggtt
     7141 gggcaagaac gaagcggtga cacccgtggg cagtccggag acggaaagat tgacgctgcc
     7201 gctgaagccg tacgacggaa caatgtagac aggggctgtg ccggtggttc cctggccgag
     7261 attgacgctg acctgggtga cgagctggaa ggttggctca tggatggtga gcggcaggat
     7321 cttcgtaacg gtgatattgc ccgaagtacc ggtgatgatg aggttggtgg tgccatcgac
     7381 cgccgtgtta ctggcattga gggtgagggt gctggttccg gatgtcgaaa ccggggagaa
     7441 cgacgcagtc acgccggcgg gcaagccgga gatggagaga ctcacggctc cggtgaatcc
     7501 gtactgaggc gttacagtga tggtggaggt gccgctgctg ccggggctga ggccgaggct
     7561 ggagggcgac ggcgtcagga gaaatgtcgg ggcatgcacg gcaagggtca cggtggtgtt
     7621 gacgctgagg ctgccggatg tgccggtgat ggtcagcggc gtggtcgacg ccggagcgtt
     7681 gctgtcggcg gtaaatgtaa gcacgctgga ggctgaggtg gggttggttc cccatgtggc
     7741 ggtaacgccg gtgggcagcg gcgtggtgac cgcgagattg acgttgccgg tgaagccgcc
     7801 ggcgtcggtg acggaaacgg tggtcgacgc cgatgcgccc tggagaacgc caacagtggc
     7861 gggcgaaccg atgatccaga aaccgggcac ctgggggccg gctaagtcgt cgataaggtg
     7921 ctgaccggta gggctccccc atccggtaac aaggtcgtag ccggcaaccg cgttatagga
     7981 gagggattgg ttctgagtat cgttgttccc ggaggtgatg tcgtggaaat cggtggagta
     8041 aggcgaaccc tgggcaagga tatagagcga aggattcagg aagccgatgc caccgagagg
     8101 agcgttgccc gcttcgacgg cctgctgatt gacgagagcc atgaagccgg cccagcgggg
     8161 agcggcgaag ctggtaccgg cccatccgct ggagcaactg ccgtaggtgc agttgtaatt
     8221 gtcctgatcg gcttccatgg agacgtcagg gacgttgcgc aaggtactgg aaccggcatt
     8281 ggaagtgttc gcgacaccgc tttgccagct gggaatggcg atgctgtcgg gactgatgcc
     8341 gccgccactg ccgataaaat tctgcgggaa gtcattccac gccgtctcat cggaccatgg
     8401 gccgccggcg ctggtagtgg tgaggtgcgt accgccgact gcggtgatgt aggcgtcttc
     8461 ctggggatag aagaatgggc tgaccgtggc gtcaaacgcg ccataatcgc ctgaggcgac
     8521 gaagaagctt tgtccctggg cggcaaattc cttgaagaag gggtcatcgg tggcggggtc
     8581 ggcaggcctc cagctccagg agcagctgat ctgcttcgcg atgttctctg aggcgatgga
     8641 gttgaggatg ttcgcatcgt cgagaccgtt gctgatgtag acgcgcacct ggctgaggcc
     8701 tggagccatg ccgatggcct gcacgatatc cagcacgacc tcggcttcgc cgctgctggt
     8761 gggtgcaccg ctcgcaccgt cgagcaacac attattcaca ggaacgttgt aagtctggcc
     8821 ggcattggta aaggtcgcgt ttacgtcgct caggttgtaa ccatcaaact ccagcagagc
     8881 gacggcctgg ccattgccat cgagggtggt tccgccgtag taagcggcgc gcatatcgct
     8941 gccgaggtaa gagccgccgg ggccggagcc gctgccgctg gccagcagag cggtttcggg
     9001 cggaggcgga atgaccatgg cctgcggaag cgagtagctg tcgaggccgg agatatgcgc
     9061 gaccggcacg ctgagattga gggacggctc gcggtcgagg gagaagaagg tgcggttctc
     9121 aaccggatgc tgatagacat tcatccggac attgaaggcg gcttcgatct gactgacggc
     9181 gccgctcagc ggcacgacga gacgattcgc gggcgtgcca gtgacggtga agccgttggc
     9241 ctgggcatag gcaaggacgg cctgatagtc ctgctgcgtt ggcccgtact gggcggtgaa
     9301 ctgatcgacg gtgaggaact tgtgaaagtc ggggctggag ggatcgtaga ggcgagtgag
     9361 gaaggcgttg agctgggcct gattgcgcag gggcagaacg agggagaagt tcacctgctg
     9421 gccgaggggc atggcgctga tgaggggcgc ctgtccggca gagacttcag gacgcacatt
     9481 gttttggagc gtctggatgg actgagcgaa gccggaggaa gcgagggcgc agccgagaac
     9541 tgctgccacg acgagccaga gccgtgaaag ccgcttaaga ctcagttttg gagaagagga
     9601 attgaagcgc agatacgaag ccggcgtgca actggaacca acgcagaaag acacaggtat
     9661 gccccatcct gaaaaatgcg accgtttaag cggccgcaat tcaaagcgag ggggaagcga
     9721 ccacgagaaa ccaatgcggt gcctctggcc gatggaaggc tccgcagggt cttgtttagt
     9781 ttgagattcg ggggaagtct atcacgctga agctggattg ccaggaagca caatcgtaat
     9841 gcgcagaggt tgcaatttga gtgactgagt tatagcttcc tgagcggatt ctgcatcact
     9901 cgtaaccatc gtggcgaaac cccaggggtc tttctgtttc gggatggagc tccgcaattt
     9961 atttatctgc gcgaagtaga gaagcaggtc tcgcggttcc gctgcgcttc ggtcgggatg
    10021 acaaggagag cgggcttccg ggacaccgct ctagtgcagc acaccgattg tatggagcca
    10081 ggttgcggcc agagcaggcc agtgggtgat gggtaattcg gtggggcgca ggccgtagcc
    10141 gtggccgcct ttcgtgtaga catgcagctc ggcagggaca gctgcttttt tcaaggccag
    10201 gaaatactgc gtgacatttt cgacgtggac gggatcgtct tcggcctgga ggagaaaagt
    10261 cgcgggcgtt gcggaagtta ccgggatgtc ggggttgaag gccatgtttt tatccgcgag
    10321 cgcgagatag ccaggataga tgatgaaagc gaagtcgggc ttgcagcttt gatcgtcggc
    10381 ggcatcgatg tggggataga ggcgctggtc gtaatgggtg ctgagggcgg cggagaggtg
    10441 agcgccggcg gagaatccca ggacgccgat gcggtgagga tcgatcgccc agtcggaggc
    10501 gtgtgcccgc gcgatgccga gcgcgcgctg cgcgtcctgc agggcggctg aggatttggg
    10561 atacgggccg gtgtcgggta cgcggtactt cacgaggagg cagttgacgc cgatggagtt
    10621 gagccagtcg cagacttcgg tgccttcgag gtcgatggcg aggatgcgat aaccgccgcc
    10681 gggaaagacg acgacggctg cgccggtgtt attggttttc gaggccaggt agagagtgat
    10741 ggtcgggacg gttacgttgc cgaggcgtac gacggcgcgt ccggctacgg ttggctcttt
    10801 cgcggtggtc gtatcgtgtt cggggccggg tggtgtggcg ggcgttggga acgctccggg
    10861 ttcgacggcg ggcgtgggtg ctccgtgagg ccagagagtg atggtggcgt gctccggacc
    10921 gggaggccag ccggatgcct gtgaatagca ggctgtgcct gcagcaagca aagcagtgag
    10981 aaccgcaaca gacacgcgca tggccgagaa tcctccccgg acatcataag tccaggtaac
    11041 ggccggctcg cttgtacgag tttccgaaat ttctcaatca gtgcgcacaa agaaaagcag
    11101 gtccctccgc ttccccttcg ctacgctcag ggtccgggtc gggatgacaa ggttttctag
    11161 tgtgctgtcc cggaaattct ctgaaaaagt cgaggataag aggtgaacga agagaaagcc
    11221 tcaggggctg aagcccgcat catttttgcg cctttgcggc accactgaag tcatgccctg
    11281 ttacaaaagc gcccggccaa ttcttcaacg aacttgaggg acgccacact agtgcgggtt
    11341 gtcgaggggg ttagggccgt tatctgtcga gttatttggc tgtggctggg aaggcttcgt
    11401 ttgc
//
LOCUS       DASZYN010000214         3184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_199138, whole genome shotgun sequence.
ACCESSION   DASZYN010000214 DASZYN010000000
VERSION     DASZYN010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199138"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1507)
                     /locus_tag="VHE33_05530"
     CDS             complement(140..1507)
                     /locus_tag="VHE33_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HVT96948.1"
                     /translation="MSEFLLFHGVAIVVLILANGFFVAAEFALVSVRDTRIEQLIAER
                     KPGARIVRRLQENMGDFLPAVQLGVTLCGLALGWLGEPAIAGAIEQPLSGFRHGHMYA
                     HFVAVPLAFALITYFDVLLGELVPKALALQRADQIAVAVAGPMDVFIRLARPIVRLMN
                     RSAVLVLRIFRAPFTNEGAVHSPEELKLMATAARRMGMLPVFQEALIHRAVEVNEVVT
                     REIMTPRQRIFSLPSDMLVEEASARIVEEQHSRVPVYDAARGPEAIVGVVYSKDVSRL
                     MHFRATASTRFSTAPLTELRLRQVMREVLVVPETKPVLDLLHEFQQRRRHMAIVVDEF
                     GSTVGLVTVEDAIEQIIGEVDDEFDVAQRAMLAATTGAMVLDGSVSLRDLETQMNWHL
                     PRDGGVETLAGFILTRLGRIPRGGECLEFEGRRLTVTEMSGRRISKVRVERLERETEP
                     EKAAG"
     gene            complement(1613..2719)
                     /locus_tag="VHE33_05535"
     CDS             complement(1613..2719)
                     /locus_tag="VHE33_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96949.1"
                     /translation="MPFRLQHAVAALSLALPLTLHAGDYTYQQTTQITGGSLLHMMKT
                     VGMFSSQARHMGDPVVSTIYLKDNRLANVSAETIQIIDLDKETITDIDVQKKTYTVMT
                     FAQMKQAMENARAQMEKQAASKPSQPAANPDAQNVKMSFDVKVRNTGAQKQISGLQSS
                     ESIMTLMMKATDSQTQQSGNMSVTNDMWMVPEVPGYSQVSDFYQRFGAKMADASVGLG
                     FDFSKMLAQNPAAGEALNDMAGEMQKLHGVPIMQIMRMGTTANGQPLPAASEAPLPAD
                     ASPSGPSAGQVAKAGISSMIPGHFGFGKKKDNPPEKNADQNSNSAQGQPTSAILMETQ
                     ITTSNFSSAPVDPSHFEVPAGYRLVQPPAATASR"
     gene            complement(2962..>3184)
                     /locus_tag="VHE33_05540"
     CDS             complement(2962..>3184)
                     /locus_tag="VHE33_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96950.1"
                     /translation="LKVLVNGGYYDLATPFYAAQYEDKHLPISSKLAENISYDWYESG
                     HMVYVKEDCLKQLHDNVAQFIKGTQAGNM"
BASE COUNT          552 a          940 c         1073 g          619 t
ORIGIN      
        1 gggcgcgcgc cctcgcccag catctgctcc accgggtcgc cgggcacgat atggatgagc
       61 aaaaagacga tcgagaccac gagccacagc accggcagcg tgaacccgag gcgcatcgcg
      121 agcgaccgca ggagtcgttt catccggcgg ctttctccgg ttctgtctcg cgttccagcc
      181 gctccacccg cactttgctg atgcggcgac cgctcatttc tgtcacggtg aggcggcggc
      241 cttcgaattc aaggcactcg ccgcccctgg gaatcctgcc cagtcgcgtc aggatgaagc
      301 cggccagggt ttccacgccg ccatcgcgcg gcaggtgcca gttcatctgc gtttcgaggt
      361 cccgcaggct cacgctgccg tcgagcacca tggcgccggt tgtcgcggcc agcatggcgc
      421 gctgggccac gtcgaactca tcgtccacct ctccgatgat ttgctcgatc gcgtcttcga
      481 ctgtgaccag gccaaccgtc gatccaaact cgtcgaccac gatggccata tggcggcggc
      541 gctgctggaa ttcgtgcagc aggtcgagaa cgggcttcgt ttcgggcacc accagcactt
      601 cgcgcatcac ctgacgcagg cgcaactcgg tcagcggggc ggtggagaag cgcgtggagg
      661 cggtggcgcg aaagtgcatc aggcggctga cgtccttgga gtagaccacc cccacgatcg
      721 cttcgggacc gcgcgcggca tcgtaaaccg ggacgcggga gtgctgctct tcgacgatgc
      781 gggcgctggc ctcttccacc agcatgtcgg aaggcaggga aaaaatgcgc tgacgcggcg
      841 tcatgatctc gcgcgtgacg acttcgttga cctcgaccgc gcgatggatc agggcctcct
      901 ggaagacagg cagcatgccc atgcgccgcg ccgcggtggc catcagcttg agctcttccg
      961 gcgaatggac tgcgccctcg ttggtaaacg gcgcgcggaa gatgcgcaga acgagcacgg
     1021 ccgaccggtt catgaggcgc acgatgggcc gggcgaggcg gatgaacaca tccatcggcc
     1081 ccgcgaccgc gactgcaatc tggtcggccc gctgcagggc cagggccttg ggcaccagtt
     1141 cgccgagcag cacgtcgaag taggtgatga gggcgaaagc cagcggcaca gcaacaaaat
     1201 gcgcatacat gtgcccgtgg cgaaagccgc tgagcggctg ttcaatggcc ccggcgatgg
     1261 ccggctcgcc cagccagccg agcgccagcc cgcagagggt gacgcccagc tgcacggcgg
     1321 gcaaaaagtc gcccatgttc tcctgcaggc ggcggacgat gcgggcgccc ggcttgcgtt
     1381 cggcgatgag ctgctcgatg cgcgtgtcgc gcacgctgac cagggcgaac tccgcggcca
     1441 caaagaagcc attcgccagg atcagaacga caatggcgac gccgtggaag aggaggaact
     1501 ccgacattgg ctccgagtct atcgcgtttg ccggtccgct ccgatgcccg gacggctgcg
     1561 ccgcatccct ggtttcggga tatgcgcaat caccgtccgg atctttggtc cgctatctgg
     1621 acgcagttgc cgcgggcggt tgcaccagcc tgtagcctgc gggaacctcg aaatgcgagg
     1681 ggtcaacggg agctgaggag aagttcgacg tggtgatctg ggtctccatc aggatcgcgc
     1741 tcgtgggctg cccctgggcg ctattgctgt tctggtcggc gttcttctcg gggggattgt
     1801 ccttcttctt accgaagccg aagtggccag ggatcatgga ggagataccg gctttggcta
     1861 cctgaccggc gctgggaccg gatgggctgg cgtcggcggg aagaggcgct tccgatgccg
     1921 cggggagagg ctggccgttg gccgtggttc ccatgcgcat gatctgcatg atgggaacgc
     1981 cgtggagttt ttgcatttcg ccggccatat cgttgagagc ctcacctgcg gctggattct
     2041 gagccagcat tttgctgaag tcgaaaccga gcccgacgct ggcgtcggcc attttggcgc
     2101 cgaagcgctg gtagaaatcg ctcacctggc tgtagcccgg gacctcgggc accatccaca
     2161 tgtcgttggt gaccgacata ttgccggact gctgtgtttg ggagtcggtg gccttcatca
     2221 tgagcgtcat gatcgattcg ctgctctgaa gcccactgat ttgcttttgg gctccggtat
     2281 tgcggacctt aacgtcgaag ctcatcttca cattctgcgc atccgggttg gcggcgggct
     2341 gagacggctt gctggcggcc tgcttctcca tctgcgcgcg ggcgttctcc atggcctgct
     2401 tcatctgcgc gaaggtcatc acggtgtagg tcttcttctg gacgtcaatg tccgtgatgg
     2461 tttctttgtc gagatcgatg atctggatgg tttccgccga aacattggcg aggcggttgt
     2521 ctttgaggta gatggtcgag acgacgggat cacccatgtg gcgggcctgg gagctgaaca
     2581 tgccgacggt cttcatcatg tggaggaggg agccgccggt gatctgcgtc gtttgctgat
     2641 aggtgtaatc gcccgcgtgc agggtgaggg gaagagcgag ggacagcgcg gcaacagcat
     2701 gctgcaagcg aaatggcata cgggaagggt ctccaagggt gaatagcagc ggcagagaaa
     2761 gagaccagcg gagcgccgcg actccgcagt ctatccagag ggattgtgtg cgtcaacggt
     2821 ctggcgttac aggggggatt cggggacgtt cacgcttccg aggccgccag aggaggccgt
     2881 gttttaagag gaaagggctg ccggatgcgg cagcccttcc ttagttttgc tgcgagttgg
     2941 tttgggtagc cgggagcgaa gctacatgtt gccggcttgg gtgcccttga tgaactgcgc
     3001 gacgttgtcg tgcagctgct tcaggcaatc ctccttcacg tagaccatgt ggccggactc
     3061 gtaccagtcg tagctgatgt tctcggccag cttgctcgag atcggcaggt gcttgtcctc
     3121 gtactgagcc gcgtagaagg gcgtggccag atcgtagtag ccgccgttca ccagcacctt
     3181 cagc
//
LOCUS       DASZYN010000215        10031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_715771, whole genome shotgun sequence.
ACCESSION   DASZYN010000215 DASZYN010000000
VERSION     DASZYN010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10031
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715771"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /locus_tag="VHE33_05545"
     CDS             complement(<1..1365)
                     /locus_tag="VHE33_05545"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HVT96951.1"
                     /translation="MVRMCCVRCLAAAILLLLALPALAQSPVPSPYDSVDPFIGTGGE
                     GNVFPGATLPFGMIQWSPDTGTDGWYRAGKDRIYGFSLTHLSGAGCPMYGDFPVLPWP
                     GELTVSPHADRALYTAPFSHSKEEAHPGYYAVTLENGTKVEITVSERAGIARFTFPQG
                     VPARLLLNAGGSANSGIIDREPKNVARSNDGFAIRIVGKDAVEGEARSGAFCNSPTRY
                     TVYLAAQFDQPFTRTAMWHDDAVDASAHDENARHAGAWLDFGDVHEVTMKAGVSFVSV
                     DGAWGNLRPTTSGDDDFESIRHSARMKWLRMLDLFRVEGGSPEQRKIFYTGVYHMLLS
                     PNLFGDGEYVGFDGERHKILPYNGTCNGPHCWSTQYANFSDWDTYRNVIQMHTLLDPD
                     LGSDEAQSLVNDAQQSGWLPRWPAANDVTYVMGGDSPTILLADAYAFGARHFDAHAAL
                     QYMLK"
     gene            1445..2077
                     /locus_tag="VHE33_05550"
     CDS             1445..2077
                     /locus_tag="VHE33_05550"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HVT96952.1"
                     /translation="MEHRPRHQCLIYSGAPSRQLPALAATLREKLRHNYRCLYLNSPP
                     MVAGMRSALWAIGVDLDVETARGSLILTSDRPHLIDGRFDVEHMMQLLTLAVEEAKRA
                     GYVGLWATGDMTWELGSDAHFMKLVEYEWRLEKLFQQHPELGGICQYHKDTLPRGMLR
                     QGLVVHPGLFISETLQLVNPEYVRPEHFSAASLRSAALDSAIDHLCGPGD"
     gene            2354..3475
                     /locus_tag="VHE33_05555"
     CDS             2354..3475
                     /locus_tag="VHE33_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HVT96953.1"
                     /translation="MLALVLPAVLAAGSGLAAQVLPSFPSVTVAPDRTVTFRYRDPGA
                     TAILCSVENVPQSLEMKKDAEGIWTATTEPLPPGIYRYHFEINGISFIDPANPWISNS
                     QMRPASMLEIPGNGPELWDAADVPHGEVHHHFYTTHIVQGLPNNQSDYWVYTPPQYDP
                     AKSYPILYLLHGWTDNAYGWLYNGRANVILDNLLAQGRIRPMIVVMPLGYGDLAFIHS
                     KIDVWQDPPSIDHNRDLYEKAFLTEVMPRVQAEYKLSQDRRDHAIAGLSMGGLEAVTI
                     GLAHPGYFGSVIGLSAAVQKLDFAKQFTGLDAKTANLDLLWIACGTGDGLIGPNRKLV
                     AFLKEKQLPVTPIETPGMHTWLVWRDNLIHFAPLLFQRH"
     gene            3545..4150
                     /locus_tag="VHE33_05560"
     CDS             3545..4150
                     /locus_tag="VHE33_05560"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HVT96954.1"
                     /translation="MKLPLPGEDLVALIVAVSFAAGLNVYATVATLGLLAHAGVLVLP
                     DALQLLASWWIIGASAALFGVEFFADKVPAFDLVWNALHTFIRIPVAALLAWRATATL
                     SPAEQLGAALLGALIAFAAHGGKTAIRAAVTPSPEPFSNMGLSFGEDVLAIGLTWLAT
                     RHPLIAGSIVAVLVLVIVLLVRWVWRALRRLFRGAEHAVQP"
     gene            complement(4190..4882)
                     /gene="mtgA"
                     /locus_tag="VHE33_05565"
     CDS             complement(4190..4882)
                     /gene="mtgA"
                     /locus_tag="VHE33_05565"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HVT96955.1"
                     /translation="MPRPKAPRRRSLFRRLLQVLILAVGGLWLMAALTLVAYRWIDPP
                     TTAVHVQRRFQAWTSNKPYHARYDFVPLRQISPNLQHAVIAAEDARFYQHHGFDWHAM
                     ELAADNDMEGGRLRGGSTLTQQLVKNLFFGTGRSILRKGAEASLVPVAELVLGKQRIL
                     ELYLNEVEWGPGVYGADAASHYYYRTAPHNLDADQAARLAAILPSPRRRRPERMNNYS
                     AIIQERERQMGW"
     gene            complement(5112..5396)
                     /locus_tag="VHE33_05570"
     CDS             complement(5112..5396)
                     /locus_tag="VHE33_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96956.1"
                     /translation="MSSIMSFMPVVWIVWAAVITFLLVLLVYRSNLTRYEEDQIFLDD
                     AGSHQKKEQEDLMVRVNKIQPLIRVVTGATCLLTVGILGVYVWDALQHLM"
     gene            5751..6176
                     /locus_tag="VHE33_05575"
     CDS             5751..6176
                     /locus_tag="VHE33_05575"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HVT96957.1"
                     /translation="MAIRMAAAVTVCAALLWVAGCNWNGKPESDQQIQQQAKQATEKA
                     KVEAQKAAAQARVAAAEAARQAHDVAVGVKAGLHSGKSPVDLNTASRAQLETLPGVTA
                     TTARRIEANRPYDTTHDLVKKGAVSEDEFGRIAGDVVVK"
     gene            6295..6903
                     /locus_tag="VHE33_05580"
     CDS             6295..6903
                     /locus_tag="VHE33_05580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HVT96958.1"
                     /translation="MTPPKKYVCVFCASAAGASPVYLEAAGELGRRIAQRGYGLVYGG
                     ATVGAMGAVADAALAAGGKVVGVIPDVIREREIDHKGLTELHVVRTMHERKAMLAERA
                     DAFVALPGGYGTMDEFIEIVTWAQLKIHAKPCVLVNVNGFWDRLLAFFDTCVAEGFIQ
                     EENRGLVQVAHDPEEALAIVERTWRERAEIPAHDERLNEVVR"
     gene            7310..9199
                     /locus_tag="VHE33_05585"
     CDS             7310..9199
                     /locus_tag="VHE33_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HVT96959.1"
                     /translation="MTADPAAPDAPAVYLFREETVDDKLHYHHLYAQIKILNDKGKEK
                     YSDIEIPYEAGTTNVRSIEGRTIHPDGTIVQFTGKPYTKELVKTGNTKIMARVFSMPE
                     VTVGSILEYAYDLQYDDNIVISPDWDLQQPIFVHHAHYRFVPATFSLGEISSKDQFGK
                     TKSATQLLYYPSLPPGDKVVRSIDGTYDLSVDNIPALPEEDYSPPIKSFAYRLVFYYS
                     SGSSGAEYWKTIGKEWSKDVDRFANPDAVRQAVAGIISPTDSDDQKLHKIYAAVMTVE
                     NTRFTREHSAAEDKAEGLRVKNAGDIWAQKRGSDDEITRLFIAMARAAGFKASAMAVT
                     ERQRNILNTGYLDWGQFEDEVAIVNIGGKDVYFDPGQRYCEYGKLHWMHTDIVGVREA
                     DNGIAPLITPAGSYADNETIRNADLELAPDGSLKGTIRVSMKGAEALRWRLNALTADE
                     QEAKSDFEKEIQERVPAGVTVKMNHFVGMSELNSALLAVLDVSGSMGTVAGKRVIMPS
                     SFFQARVTPLFAETKRESPIDLRYPYADEDRVNIKLGPGLSLEGLPTDASVPMEKLAA
                     YSVKYKNDANTYTEDRVIALGAARFKKEEYPELRDFFQKAGAQDGQQLVLKRAAVETA
                     SGSAQ"
     gene            9220..>10031
                     /locus_tag="VHE33_05590"
     CDS             9220..>10031
                     /locus_tag="VHE33_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HVT96960.1"
                     /translation="MSRPATHPIAPLQSFVKLGFFAVAGLCALLCTPAARAAKEQPVP
                     DWVVQASTAAPLKPEWHDAKAVYLLADTLITVQPDGRVISRYRAVVKILRPQGRDYAT
                     PVASFSSDTKLQSFHVWSIGPDGHRYSMKDSEYIEAGDPAYGMLYVDIRHKIASPPAA
                     DPGAVVAWESIKQMPSYLSEDTWGFQNAIPTQHSVYEIDLPSGWHEEAVWSHHAPLQP
                     AQPAPNQFRWEQQNIDGIDLDNVALPPAWEALAGRMTVHFAQDPLPQGEALWA"
BASE COUNT         1989 a         3241 c         2941 g         1860 t
ORIGIN      
        1 cttgagcatg tactgcagcg cagcgtgggc atcgaagtgg cgcgcgccaa aggcgtacgc
       61 atcggcgaga aggatcgtcg gcgagtctcc gcccatcacg taggtgacgt cgttggcggc
      121 cggccagcgt ggcagccatc cggactgctg cgcatcgttg accagcgact gcgcctcatc
      181 cgacccaagg tccgggtcga gcagcgtgtg catctggatc acgttgcggt acgtgtccca
      241 gtcggagaag ttcgcatact gagtgctcca gcaatgagga ccattgcacg tgccgttgta
      301 gggcaggatc ttgtgccgct cgccatcgaa gcccacatac tcgccgtcgc caaacaggtt
      361 cggcgacagc agcatgtgat acaccccggt gtagaaaatc ttccgctgct ccggcgatcc
      421 gccctccaca cggaagaggt ccagcattcg cagccacttc atgcgcgcgg agtgccggat
      481 cgactcaaaa tcatcgtccc ccgatgtggt cggcctcagg ttcccccacg caccgtcgac
      541 gctcacaaat gagactccgg ccttcatggt cacttcgtga acatcgccga agtccagcca
      601 cgcgccggca tgccgcgcgt tctcgtcgtg cgcggacgca tccaccgcat cgtcgtgcca
      661 cattgccgtc cgcgtgaacg gttgatcaaa ctgcgcggca agatacaccg tgtaccgtgt
      721 cggcgaattg cagaatgccc cggagcgcgc ctccccctcc actgcatctt tcccgacaat
      781 ccgaatcgca aacccgtcgt tcgaccgagc cacattcttc ggttcccggt cgataatccc
      841 cgaattggcg ctcccgcccg cattcagcag cagccgtgcc ggcactccct gggggaacgt
      901 aaaccgcgcg atcccggccc gctcgctcac cgtgatttcc acctttgttc cgttctccag
      961 cgtcacggcg taatagcccg gatgcgcctc ttccttgctg tgagagaacg gcgccgtata
     1021 cagcgcgcga tcggcatgcg gactcacggt cagctcgccc ggccacggca gcacggggaa
     1081 atctccgtac atcgggcacc ccgctccgct caggtgcgtc agcgaaaacc catagatgcg
     1141 gtccttgccg gcgcggtacc agccgtccgt ccctgtgtcg ggactccact ggatcattcc
     1201 aaacggcagc gtcgccccgg gaaaaacgtt tccttcgccc cccgttccga tgaacggatc
     1261 gaccgaatcg tagggagagg gaaccgggga ttgagccaag gcaggaaggg cgagcagaag
     1321 aagaatggcc gcagccagac accgaacgca acacatgcga accatagtag tccgggcaga
     1381 cgggcgtccc gtctcgatgg ggccatagca aggaatgcca ggtcgttact ataatggcac
     1441 cgttatggaa caccggcccc gtcaccagtg tctgatctat tcaggtgccc cctcgcggca
     1501 gctcccggcc ctggcagcaa cccttcggga aaaactccgc cacaactacc ggtgccttta
     1561 tctgaacagc ccgcccatgg tcgccgggat gcggtcggcc ttgtgggcca tcggtgtgga
     1621 tctcgacgtc gagactgcaa ggggttccct catcctcacc tccgatcgcc cgcacctgat
     1681 cgacggacgc ttcgacgtcg aacacatgat gcagctcctg actttggccg tagaagaagc
     1741 caagcgcgct ggatatgtcg ggctgtgggc cacgggcgat atgacctggg agctggggtc
     1801 cgacgctcat ttcatgaaac tggtcgagta tgaatggcgg ttggagaagc tcttccagca
     1861 gcatcctgaa ttaggcggca tctgccagta ccacaaggac acccttcccc gtggaatgct
     1921 gcgccagggt ctggtggttc atccgggcct cttcatcagc gaaacgctcc agctggtgaa
     1981 tcctgagtac gtacgcccgg aacacttcag cgccgcctcg ctgcgcagcg ccgctctcga
     2041 ttccgccatc gaccaccttt gcgggcccgg agactagtct agtgtgctgt cccgggcatt
     2101 ctctgaagag gtgaacgaag agaaagcctc aggggctgaa gcccacatcg tttgtgcgcc
     2161 tttgcggcac gactgaagtc atgccctgtt acaaaggcgc cggccaattc ttcaacaaac
     2221 ttgcgcgaca ccacaccagg gccgagttca cctagcgacg gctcctgcgg cggagtgctt
     2281 cgcctcagct acactggcgc gtatgcatgc acgtgctgat cgtccgacgc gaagccgccg
     2341 ttcgtgtgct tctttgctgg ccctcgttct tcctgcagtg ctggcagcag gatccgggct
     2401 ggcggcgcag gttctcccga gctttccctc cgtcacagtg gctccggatc gcaccgtcac
     2461 cttccgctat cgtgaccccg gcgcaaccgc catactgtgt tccgtcgaga acgtgccgca
     2521 atccctcgag atgaaaaaag acgccgaagg catctggaca gcgaccacag agccgctacc
     2581 gcctggcatc tatcgctatc acttcgagat caacggcatc tccttcatcg atccggccaa
     2641 tccctggatt tcgaattcgc agatgcgtcc ggccagcatg ctcgagattc ccggcaatgg
     2701 ccccgagctg tgggacgctg ccgacgtccc gcacggcgaa gttcaccacc acttttatac
     2761 gacgcacatc gtccagggcc tgcccaacaa tcagagtgac tactgggtct acacgccgcc
     2821 acaatacgat cccgcgaagt cctacccgat cctctacctg ctgcatggct ggaccgacaa
     2881 cgcctatggc tggctctaca acgggcgcgc caacgtcatt ctcgataatc tgctcgcgca
     2941 gggccggatc aggcccatga tcgtcgtcat gcccctgggc tacggcgacc tggccttcat
     3001 ccacagcaaa atcgacgtct ggcaggatcc ccccagcatc gaccacaacc gcgatctcta
     3061 cgaaaaggcg tttctcaccg aagtcatgcc acgcgttcag gccgagtaca agctctcgca
     3121 ggatcgccgc gatcatgcca tcgccggcct ctcgatgggc ggcctcgaag ccgtgaccat
     3181 cggacttgcc catcccggct acttcggttc ggtcatcggc ctcagcgcag ctgtacagaa
     3241 gctggacttc gcgaagcagt tcaccggcct cgatgcaaag accgcgaatc tcgatcttct
     3301 gtggatcgcc tgcggtaccg gcgacggtct catcgggccc aaccggaagc tggtcgcgtt
     3361 tctgaaagag aagcagctac ctgtgacgcc aatcgaaact ccgggcatgc acacctggct
     3421 ggtgtggcgt gacaacctca tccactttgc gccgctgctg tttcagcggc actgatcctg
     3481 ccggtcggca agcaaggcat aaaaggatga gtagcactac gcgcctccgc cctatactgt
     3541 ccgcgtgaaa ctcccgctgc cgggtgaaga cctcgtcgcc ctcatcgtcg ccgtctcgtt
     3601 cgccgcgggg ctgaacgtct acgccacggt tgcgaccctc gggctgctgg cccatgcagg
     3661 cgtactcgta ctgcccgatg cgttgcaact gctggcgagc tggtggatca tcggcgcaag
     3721 cgcggcgctg ttcggcgtgg aatttttcgc ggacaaggtc ccggccttcg acctcgtctg
     3781 gaacgcgctg cacaccttta tccgaatccc ggtcgcggcg ctgctcgcct ggcgtgcaac
     3841 cgcgacgcta agcccagccg agcaactggg cgccgcgctt ctgggggcgc tcatcgcgtt
     3901 cgccgctcac ggaggcaaga ccgcgatccg cgccgcggtt acgccgtcgc cggaaccgtt
     3961 ctcgaatatg gggctcagtt ttggcgaaga cgtgctggcc atcgggctca catggctcgc
     4021 gacgcgtcat cctctgatcg caggttccat tgtggctgtg ttggtgctcg ttatcgtgct
     4081 gctggtgcgc tgggtttggc gagcgttgcg ccggctgttc cgtggcgctg agcacgccgt
     4141 gcaaccctga gcgcgatcca ttgagctgcg cgcgccggct tgtgagccat taccacccca
     4201 tttgccgttc gcgttcctgg atgatggcgc tgtagttgtt catgcgctcg ggtcgtcgcc
     4261 gccggggaga ggggagaatt gccgcgagcc gggccgcctg gtccgcgtcg agattgtggg
     4321 gcgcggtcct gtagtagtag tgcgacgccg catcggcgcc gtaaacccca ggtccccact
     4381 ccacttcatt caggtacagc tcgaggatgc gctgcttgcc cagcacgagt tcggctaccg
     4441 gcaccaggct ggcctccgca cctttgcgca ggatcgaacg cccggttccg aagaacagat
     4501 tcttcaccag ttgctgtgtg agcgtggagc caccgcgcag gcgtccgcct tccatgtcgt
     4561 tatccgcggc cagttccatc gcgtgccagt cgaatccatg gtgctgatag aagcgcgcat
     4621 cctcggcggc gatgacggca tgctgcaggt tcggcgagat ttgccgcagc ggaacgaaat
     4681 cgtaacgcgc gtgatacggc ttgttgctgg tccacgcctg aaaacggcgc tgcacgtgaa
     4741 cggcggtggt cggcggatcg atccagcgat aagcaacaag cgtgagcgcg gccatcaacc
     4801 acaagccccc cacagcaaga atcaaaacct gcagcagccg ccggaacagg cttcggcgac
     4861 gcggagcttt gggacgcggc aatcgtcgtg cgagcaccct tcagaataat ggcgcccgag
     4921 cgcgtggcgg atggactggt tgtggcggag aggatcgaaa tgccgggttg tcgaccgggc
     4981 gcatccggag aacaaaagcg gcggaagaat cgcccaccgc gcgggccgga gaggtgggat
     5041 tccggccacg cgcgcgagga agagtgcatc gaaccgcggc gccgaaggtg cgccgggggt
     5101 gcaacagtgg gctacatgag atgctgcagc gcatcccaaa cgtagacgcc cagaatacca
     5161 acggtaagaa ggcaggtagc tccggtgacg acccggatga ggggttgaat cttgttgacc
     5221 ctgaccatca ggtcttcctg ctccttcttc tggtgactgc ctgcatcgtc gaggaagatc
     5281 tggtcctctt cgtagcgggt gagattcgag cggtagacca gcaggacgag cagaaaagtg
     5341 atgactgccg cccaaacgat ccaaacaaca ggcatgaagc tcatgatgga gctcataggc
     5401 gcctcgtggg ggataattcc gcatcggagc cgggaaggcg gcgaactcga gagagcgccg
     5461 gtaagcgaac aaaaaccggc tgcaaccggg agcgcgtcga ccagccgagc ctcgaaacgg
     5521 ttgagccggc tcctggtgcc acagcgggtc cggaaaccct gcatcgaaat cgggacccag
     5581 ggctcggagg gcaggagggg ccgtccggag gggaatgtta gcgctgttga cccggcgttg
     5641 aacagcgaaa ttttttgtgt cgtccgttac catctggcaa ctctccggga atcagaagat
     5701 tccatgcaag ggcgaggcaa gcgaaaatgc gggaatccag gtggacagag gtggcgattc
     5761 ggatggcggc agcggtgacg gtgtgtgcgg ccctgctgtg ggtggcgggg tgcaactgga
     5821 acggaaagcc ggaatcggac cagcaaatcc agcagcaggc aaaacaggcc accgagaagg
     5881 cgaaggtgga ggcgcagaag gcggcggccc aggccagggt agcggcagcc gaagctgcgc
     5941 gtcaggcgca cgatgtggcc gtcggcgtga aggccggact ccacagcggc aagagcccgg
     6001 tggatctgaa caccgcgagc cgcgcgcaac tggagactct gcccggtgtg acggccacaa
     6061 cggcgcgccg catcgaagcc aatcggccct acgacacaac gcacgatctg gtgaagaagg
     6121 gcgccgtcag cgaggacgaa ttcggacgca ttgcgggcga tgtggtagtg aagtagagca
     6181 gagcacaggt cacagctcac agagcacagg tcacagagcc aggtcgcaga gccgcagata
     6241 tcggcttctc cccgcttctg catttcccgt ttccggcttc gataaactca aaccgtgact
     6301 cctccaaaga aatatgtctg cgtcttctgt gcgtccgcgg cgggcgcgtc tcctgtttat
     6361 ctggaagcgg ccggtgaact ggggcgacgg atcgcccaac ggggttatgg cctcgtctat
     6421 ggcggcgcga ccgttggggc gatgggcgcg gtcgcggatg cggcgctcgc ggccggcggg
     6481 aaggtcgtcg gcgtcattcc ggatgtgatc cgggagcggg agatcgatca caaggggctg
     6541 accgaactgc acgtggtgcg caccatgcac gagcgcaagg cgatgctcgc cgagcgcgcc
     6601 gatgccttcg tcgcgctgcc cggcggatac ggcacgatgg acgagttcat cgagatcgtg
     6661 acgtgggccc agctcaaaat tcatgccaag ccgtgcgttc tcgtgaacgt aaatggtttc
     6721 tgggacaggc tgctcgcgtt ttttgatacc tgcgtcgccg aaggcttcat tcaagaagag
     6781 aatcgcggcc tggtgcaggt cgcgcacgac ccggaagaag cactggcgat cgtggagcgg
     6841 acctggcggg agcgcgcgga gattcctgca cacgacgaac ggctgaatga agtcgtacgc
     6901 tgactcccgc gcgtcgcgaa agctgcgacg tccctttgct gtcgcgtggg gaattggagt
     6961 catccgcgcc taactcgtca tcggcctgcg atcaatccct gcaaagtaac taccctttcg
     7021 tagccatctc cttctctctt gcgtgacctc cgcgcggatc ctctcccgat tatcaaaaat
     7081 cttcttgccg aaccacgctg caggacttac aaagactaca tctcccctga attggtcatt
     7141 gcgggtacaa agcgatccgc atgcaattgt tcctaacgct ggtactttgt gaggctttgc
     7201 cgtggttcgt ttgtttcgcc ctgtctttct gcctgccgct ctccttttgc tttcttcctg
     7261 tcccgtcttc gccgccacct ggagacaacc tactcccgac gaactgaaga tgacggcgga
     7321 tccggccgcg cccgatgcgc cggccgtcta tctgttccgt gaagagacgg ttgatgacaa
     7381 gctgcactac caccatctct atgcccaaat caaaatcctc aacgacaagg gcaaggaaaa
     7441 atacagcgac atcgaaattc cctacgaggc cggcaccacc aatgtccgct ccatcgaagg
     7501 ccgcacgatt caccccgacg gcaccatcgt gcaatttacc ggtaagcctt acaccaagga
     7561 gctggtgaag acgggcaaca caaaaatcat ggccagggtt ttctcgatgc cggaggtgac
     7621 ggtcggcagc atcctcgaat acgcctacga cctgcagtac gacgacaaca tcgtcatttc
     7681 acccgactgg gatctccagc aaccgatctt tgtccaccac gcccactatc gctttgttcc
     7741 cgccaccttc tctctgggcg aaatctccag taaagaccag ttcggcaaaa cgaagtcggc
     7801 aacccagttg ctctactatc cttcgctccc gccgggcgat aaggtcgtcc ggtccatcga
     7861 tggaacctac gacctctccg tcgacaacat cccggccctg cccgaggaag attactctcc
     7921 gccgatcaag agctttgcct accgtctggt cttctactat tcctccggct ccagcggagc
     7981 cgaatactgg aagaccatcg gcaaggaatg gtccaaagac gtcgatcgat tcgcgaatcc
     8041 tgacgcagtc cgacaggcgg tcgccggcat catctctccg accgacagcg acgatcagaa
     8101 gctgcacaag atctatgccg ccgtcatgac cgtcgagaac acccgcttca cccgcgagca
     8161 ttcggcggcg gaagataaag ccgagggcct gcgtgtgaag aacgccggcg acatctgggc
     8221 ccagaagcgc ggctccgacg acgagatcac gcgtctgttc attgcgatgg cgcgcgccgc
     8281 cggtttcaag gcctccgcca tggccgtcac cgagcggcag aggaacatcc tcaacaccgg
     8341 ctacctcgac tggggtcagt tcgaagatga agtggccatc gtcaacatcg gtggcaaaga
     8401 cgtttacttt gaccccggcc agcgctactg cgagtacggc aagctccact ggatgcacac
     8461 cgacatcgtc ggagtccgcg aggccgataa cggcatcgcg cccctcatca caccggcggg
     8521 gtcttacgcc gacaacgaaa ccatacgcaa tgccgacctc gagcttgctc ccgatggctc
     8581 tctcaaaggc acgattcgcg tctccatgaa gggagccgag gctctgcgct ggcggcttaa
     8641 cgctctcacc gccgacgagc aggaagccaa atccgacttc gaaaaagaaa ttcaggaacg
     8701 cgttcccgcc ggcgtgacgg tgaagatgaa ccacttcgtc ggcatgtccg agctcaactc
     8761 ggccctcctg gccgtgctcg atgtcagcgg cagcatgggc accgtcgccg gcaagcgcgt
     8821 gatcatgccg agctctttct tccaggctcg cgtcacgcct ctgtttgcag agaccaaacg
     8881 ggaaagcccc atcgatctcc gttatccgta tgccgacgag gatcgtgtca acatcaagct
     8941 cggtcccggc ctgtctctgg agggtctgcc caccgacgcc tccgtgccca tggagaaact
     9001 cgccgcttat tccgtgaagt acaagaacga cgccaatacc tataccgagg accgcgtcat
     9061 tgccctcggc gccgcccgct ttaagaagga ggaatatccc gagctccgcg acttcttcca
     9121 gaaagccggc gcccaggatg ggcagcaact cgtgttgaag cgcgccgccg tcgaaaccgc
     9181 gtcgggctct gcccaatgat cctgaccgga acaaacagca tgagtcggcc cgcaacgcat
     9241 cccatcgctc ctcttcaatc gttcgttaag ctgggattct ttgccgtagc cggactgtgc
     9301 gcacttctct gcacgccggc cgcacgcgcc gcaaaggagc agcccgttcc cgactgggtc
     9361 gtccaggcct ctactgccgc gccgctcaaa cctgagtggc acgatgccaa agctgtgtac
     9421 ctcctcgcgg acacgctgat cacggtccag cccgacggcc gtgttatcag ccgttaccgc
     9481 gccgtcgtca aaatcctccg tccccagggc cgcgactacg ccacccctgt cgcttctttc
     9541 tctagtgata caaagctcca gtccttccat gtctggagca tcggacccga cggccatcgc
     9601 tactccatga aggattcgga gtacatcgag gcgggcgatc ccgcttacgg catgctttat
     9661 gttgatatcc ggcacaagat cgcctcgccc ccggcggccg atcccggtgc agtcgtcgca
     9721 tgggaatcca tcaagcagat gccctcctat ctcagcgaag acacctgggg attccagaac
     9781 gccattccga cccagcattc cgtctacgaa atcgatctgc cctccggatg gcatgaggaa
     9841 gccgtctggt cccatcacgc ccctctccag ccagcacaac ctgcccccaa ccagttccgc
     9901 tgggagcagc aaaacatcga cggcatcgac ctcgataacg tcgcgctccc cccggcatgg
     9961 gaggctctgg ctggacgcat gacggtccac tttgctcagg atccgctccc ccagggtgag
    10021 gccctctggg c
//
LOCUS       DASZYN010000216         6991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_843743, whole genome shotgun sequence.
ACCESSION   DASZYN010000216 DASZYN010000000
VERSION     DASZYN010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843743"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VHE33_05595"
     CDS             <1..523
                     /locus_tag="VHE33_05595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT96961.1"
                     /translation="MLVRKARLQDATNIYELVNSLSGDGTLLRRPFAEICENVRDFTV
                     AESEGGIFLGCGALHLYGPHLAEVRSIVVRPEAKSHGVGGELLQSLLAEAEQNGVGCV
                     CLFTRIPDFFFRYGFREVEDRTALPDKIFKDCQNCPRLYKCDEVAMARGQVPKVSILG
                     PRIEAEQLVRLSV"
     gene            743..958
                     /locus_tag="VHE33_05600"
     CDS             743..958
                     /locus_tag="VHE33_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96962.1"
                     /translation="MAVRVVPSTFEVLDATGDAIVDPSRCVPGVAPDGLPEVNLGAFR
                     GLAFALILQTVLFLLGAVAWEFFRSLR"
     gene            1028..1243
                     /gene="thiS"
                     /locus_tag="VHE33_05605"
     CDS             1028..1243
                     /gene="thiS"
                     /locus_tag="VHE33_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HVT96963.1"
                     /translation="MDLVINGRPRSFPELAASSSLQDLVSALALKSDRVAVEQNGAIV
                     ARSSWPATPIADGDKFEIVQFVGGGSA"
     gene            1312..2316
                     /locus_tag="VHE33_05610"
     CDS             1312..2316
                     /locus_tag="VHE33_05610"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HVT96964.1"
                     /translation="MKKIRWGVLSTANIGVRKVLPAMQRGQFTTVAAIASRDLAKGRA
                     VAEQLGIPTAYGSYEELLADPNIDAIYNPLPNQLHVPWTMKAAEAGKHVLCEKPIAMS
                     AGEAHTLLAVRDRTGVLIGDAFMIRSHPQWLRMRALIDEGRIGMLRSVAAHFSYFNVD
                     PANIRNQPETGGGALYDIGCYCIQSARYAFGSQPTRVCGLIDRDPQLSTDRLTSALLD
                     FPAGHAIFTCSTQLVPHQRVNFYGTEGRIEIEIPFNAPPDRPTRLFIDDGSDITGAHI
                     VTETFPVVDQYTLQGDAFSRAILEGTPVPVSLEEGIANMAVIDAIFQSAQSGVWQPVR
                     "
     gene            3032..3436
                     /locus_tag="VHE33_05615"
     CDS             3032..3436
                     /locus_tag="VHE33_05615"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVT96965.1"
                     /translation="MTLFAPLLAGNTLGSAVNSFVIAEWSAEGTPAGSEPQSQAPLHL
                     HHSDDEAWYVLEGSLNIRLGSEVIEARAGSPVLVPRGTPHTYWNPAPEAARYLLLMTP
                     RIHALIQAIHVLPERSAPTLRALFSQHDSELL"
     gene            3456..4148
                     /gene="merB"
                     /locus_tag="VHE33_05620"
     CDS             3456..4148
                     /gene="merB"
                     /locus_tag="VHE33_05620"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046413 - organomercury catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HVT96966.1"
                     /translation="MIEPSVLSRLHHELISGLIQTGVCPTNTELALRLQATPAEIDDR
                     LGGLAEMHGVVLHPHRPAPWILHPFSLTPTLNWIEGRGRSWWAPCIWCAFGVATLVGG
                     EVRVHSRYGAECDPIVIPVRDGHPIGFESVFVHFAIRPARAWDNVHEHCSMVLPFRHI
                     EEIGRWSSRHGLPRGEAVPLHQVAALARAWYGTHADPGWRKWSVAEAQEIFRQADLTS
                     AFWNLESKEGRF"
     gene            4191..5168
                     /locus_tag="VHE33_05625"
     CDS             4191..5168
                     /locus_tag="VHE33_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HVT96967.1"
                     /translation="MFLKCATASLLVIAATVPTFAQSDSALTARLQSQIAGFHGKVAL
                     YTRDLATNKTVAINADEPVPTASVIKLGILYTALQQIRAGRAHFDDRLMLNQDDQVPG
                     SGVLLFLDTPHTLTLKDALTLMIAVSDNTATNLVIDDLGLDTIDNELTAPAPRGLALK
                     NTWLYKKVFKDANGPQPADQPKFGLGKTTAHEMASIMERFVTCNLGPAPGDASAAPGP
                     GATAIPSDQDLCKAAIHMLKVQSDRDMIPRYLEKLDTTEGDSAIADKIGALDHARNDV
                     GAVYTKHGVILISAFTHDNEDTSWTVDNTAELLIAKMAKTIVDAWAPTP"
     gene            complement(5170..5688)
                     /locus_tag="VHE33_05630"
     CDS             complement(5170..5688)
                     /locus_tag="VHE33_05630"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVT96968.1"
                     /translation="MANTINAAELKRMLGLEPHPREGGWFRETWRAEESIPLEALPTG
                     RYRGARSAGTAIYYLLEPGNFSEMHKLASDEVFHFYLGDAVEMLQLWPDGTGKRVVLG
                     QDLAQGQRLQTVVPQGVWQGTRLIPGGTVALLGCTVSPGFDYADYASGAREALTLGWP
                     EWAEMIGQLTRE"
     gene            5935..6648
                     /locus_tag="VHE33_05635"
     CDS             5935..6648
                     /locus_tag="VHE33_05635"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HVT96969.1"
                     /translation="MFEDSTFASANRIHTRSSRYAAGSLFLQTSLLAILVLIPFLYPN
                     ALPPKFLSVSLIAPTPPPAAPVVEPQHAIASTSHTELLTSTIQAPSTIPTRIPHIVDS
                     GPPGAIPSSAFANSGTNTLPLGPATPSVTPRVQTAKHTGPIHISQGVAAGQLIVPIQP
                     RYPPIARATGTQGTVMVSAIISTDGRSESLRVLSGPPMLIPAAVDAIRQARYRPWTLN
                     GAPVEVETTINVVFSLGND"
BASE COUNT         1281 a         2681 c         1689 g         1340 t
ORIGIN      
        1 catgctggtg cgtaaagcgc gtctccagga cgccaccaac atctacgaac tggtcaactc
       61 gctctccgga gacggcaccc tcctccgccg ccccttcgcc gagatctgcg aaaacgttcg
      121 cgacttcacc gtcgctgaat cggaaggcgg catcttcctc ggctgcggcg cgctgcacct
      181 ctacggcccc cacctcgccg aggtccgctc catcgtcgtc cgccccgaag caaaaagtca
      241 cggtgtcggt ggcgaattac tccagtcgct actcgctgaa gccgagcaaa acggcgtcgg
      301 ctgcgtctgc ctcttcaccc gcatccccga cttcttcttc cgctacggct ttcgcgaggt
      361 tgaggaccgc accgccctcc ccgacaaaat tttcaaggac tgccagaact gcccacgcct
      421 ttacaagtgc gatgaagtcg cgatggctcg cggccaggtt cccaaggtct cgatcctcgg
      481 accccgcatc gaggcggaac aactggtacg gctgagcgtt taagtcttcc tggcatgacg
      541 cacgctggct tcccgtggat tgccgatgag gcgaatcgcc ggataccccg cgctatgcga
      601 tgacatggcg ttctacctgc cgatccgctt tgggaatgcg cgatccaggc gtcctgtttt
      661 ggaacacgtt cgccacgatt cccccatcaa ttcaagccaa ggccgctcgc ggtcgatacc
      721 ttcacttacg aggggatctc ttatggccgt gcgtgttgtt ccatccacgt ttgaagtgct
      781 cgatgccacc ggcgacgcca tcgtcgatcc atcccggtgc gttcccggcg ttgccccaga
      841 tggtctcccc gaggtgaacc tcggcgcctt ccgcggtctc gcttttgcac tgatccttca
      901 gaccgtcctc ttcctgctgg gtgccgtcgc ctgggaattt ttccgttcgc tccgctgatc
      961 gtgctggggc gaatccgctg ccacatgggc gttacgccac tggccggcgc tgcgctactc
     1021 tctctccatg gacctcgtga tcaacggacg cccccgctcc ttccctgagc tggccgcatc
     1081 ctcctcgctc caggacctcg tctctgccct cgctctcaag tccgaccgcg tcgccgtcga
     1141 acagaatggc gccatcgtcg cacgttcctc ctggcccgca actccgatcg ccgatggcga
     1201 caaatttgag atcgtgcagt tcgtcggcgg tggctccgcc taaccgcccg gcaatctttt
     1261 gtccgggaat tcctgcaatc ttccatacac ccgcccaatt accctgttgc catgaagaag
     1321 attcgctggg gcgtcctctc cacagccaac attggcgttc gcaaagtcct tcccgccatg
     1381 cagcgcggcc agttcaccac cgtggccgcc atcgcttcgc gcgacctcgc caaaggccgc
     1441 gccgtcgccg aacagctcgg catccccacg gcctacgggt cctacgaaga actccttgcc
     1501 gaccccaaca tcgacgccat ctacaacccc ctccccaacc agctccacgt tccctggacc
     1561 atgaaagctg ccgaagccgg caagcacgtt ctctgcgaaa aacccatcgc catgagcgcc
     1621 ggcgaagccc ataccctcct tgccgtccgc gatcgcaccg gcgttctcat tggcgatgcc
     1681 ttcatgattc gttcccaccc gcagtggctg cgcatgcgcg ccctgatcga cgaaggccgc
     1741 atcggcatgc tgcgctccgt cgctgcccac ttcagctact tcaacgtcga tcccgccaac
     1801 atccgcaacc aaccggagac cggcggcggc gcgctctacg acatcggctg ctactgtatc
     1861 cagtccgccc gctacgcctt cggctcgcag cccacccgcg tctgcggcct catcgaccgc
     1921 gatccccagc tcagcaccga ccgcctcacc tccgcgctcc tcgacttccc tgccggccac
     1981 gctatcttca cctgctccac ccagctcgtc ccacaccagc gcgtcaactt ttacggaacc
     2041 gaaggccgca tcgaaatcga gatccccttc aacgcgcctc ccgatcgtcc cacacgcctc
     2101 ttcatcgatg acggcagtga catcaccggc gcccacattg tcaccgagac attccccgtc
     2161 gttgatcagt acaccctgca gggcgacgcg ttctcgcgtg caattctcga aggcacgccc
     2221 gtccccgtct ccctcgaaga gggcattgcc aacatggccg tcatcgacgc aatcttccag
     2281 tcagcgcaat ccggagtatg gcaaccggtg cgctgatcgc gcatgaagat cgtccgccag
     2341 accgaaggcg aacttatcgt ccaggacagc agcgtctgga tgggcgtcct gctcgcagca
     2401 tgtagcctgt ttccgctcta cattgccctg acccacgacg agcaccggct ttttgtcgcc
     2461 tttggatgcc tgcttctctt cgctatcccc tggattcgcc gcgccacttt caccttcgac
     2521 gccgccacgc aaaccattcg ctggaaccgc atgcgctacc tcatcacccg caccggctcc
     2581 ctacccttct ccgacattca ggccgtcgat atccagtcca ccagccgcgg acagtccaac
     2641 gtgaacatct accgtcttgc actcgttact cgtcagggaa cgatgcccat gtccgacgtc
     2701 tacagcagcg gcgaaagtct gtatacgaac gcccgcgacg ccatccagcg ctttctcaaa
     2761 ctcgagcgaa attcaaccaa cacctccatc gccgccggca tcgacatctc cattcgcgct
     2821 gcaacagggc cgcaaaatcg atgccattca gctcctgcgc agcaccgaaa acctctccct
     2881 cactgccgcc accaggcgca tcgatgctat cgacgcctcc atgatttccg tcgcccggta
     2941 gggtcccgca aatttcgccg gcggcgacac atgccgctgc aagcccgtat tctgttgagc
     3001 aatgtcccag cctccgctca gaggtctacc cgtgaccctt ttcgctccgc tgctcgccgg
     3061 caatactctc ggctctgccg tcaacagctt cgtcatcgca gaatggtccg cagagggcac
     3121 acccgcagga tccgaaccgc agagtcaggc cccgctccac ctccaccaca gcgatgacga
     3181 agcctggtac gtactcgaag gatctctgaa catccgcctt ggcagcgaag tcatcgaagc
     3241 tcgcgccggt tcccccgtcc tcgttcctcg cggaacgccg cacacctact ggaatcctgc
     3301 tcccgaggcc gctcgctatc ttcttctcat gacaccgcgc atccacgcgc tcatccaggc
     3361 catccacgta ctgcctgaac gcagtgcgcc aaccctgcgc gcactgttca gccagcacga
     3421 ctcagagcta ctctagcctc gtgaacgtcg catccatgat cgaaccatcc gtcttgtctc
     3481 gtctccatca cgaactcatc tccggcctca ttcaaaccgg tgtctgtccc accaacaccg
     3541 aactggccct tcgcctccag gccacccccg cagaaatcga cgacaggctc ggcggactcg
     3601 cggaaatgca cggagtcgtc ctccatcccc accgtccagc gccatggatc ctccacccct
     3661 tttcgctcac gcccaccctc aactggatcg agggccgcgg ccgcagctgg tgggctccct
     3721 gcatctggtg cgccttcggt gtcgccactc tggtcggcgg cgaagttcgc gtacactccc
     3781 gctatggcgc cgaatgcgac cccatcgtca tccccgtgcg cgatggacat ccgatcggct
     3841 tcgagtccgt cttcgtccac ttcgccatcc ggccggcgcg cgcctgggac aacgtccacg
     3901 agcactgctc catggtgctt cccttccgcc atatcgaaga gatcggacgc tggtccagcc
     3961 gccacggtct cccgcgcggc gaggccgttc cccttcatca ggtcgcggcg ctcgcccgcg
     4021 cctggtacgg aacgcacgcc gatccgggat ggcgcaagtg gagcgtcgcc gaagctcagg
     4081 agatcttccg ccaggcggac cttacgtccg ccttctggaa tctcgaaagc aaagagggcc
     4141 ggttctagtc agcgcctcgc tcccatcgct gctcggctac actggccagc atgttcctga
     4201 aatgcgcgac ggcttcactc cttgttattg ccgccaccgt tcccacgttt gcgcagtccg
     4261 attctgccct caccgcgcgg ctgcaatcgc aaatcgccgg cttccacgga aaagtcgccc
     4321 tctacacgcg cgacctcgct accaacaaaa ccgtcgccat caacgccgac gagcccgtgc
     4381 ccaccgcctc cgtcatcaag ctcggcatcc tctacaccgc gctccagcag attcgcgccg
     4441 gccgcgccca ctttgatgac cgcctcatgc tcaaccagga cgaccaggta cccggctccg
     4501 gcgtccttct cttcctagat acgccccaca ccctcaccct caaagatgcg ctcactctca
     4561 tgatcgccgt cagcgacaac accgccacca acctcgtcat cgatgacctg ggtctcgaca
     4621 ccatcgacaa cgaactcacc gcgcccgcgc cgcgcggtct cgcactgaaa aacacatggc
     4681 tctacaaaaa ggtcttcaaa gacgcgaacg gtccccagcc cgccgatcag ccgaagttcg
     4741 gtctcggcaa aaccaccgcc cacgaaatgg cctccatcat ggagcgcttc gtcacctgca
     4801 acctcggccc ggcacccggc gacgcctcgg cagcccctgg cccaggtgca accgccatcc
     4861 cgtccgacca ggatctctgc aaagccgcga tccacatgct caaggtgcag tccgaccgcg
     4921 acatgatccc gcgctacctg gaaaagctcg acaccaccga aggtgattcg gccatcgccg
     4981 acaaaatcgg cgccctcgat cacgcccgca acgatgttgg cgccgtttac acaaagcacg
     5041 gcgtcatcct gatctccgcc tttactcacg acaacgagga caccagctgg accgtcgaca
     5101 ataccgccga actcctcatc gcaaagatgg caaaaacgat cgtcgacgcc tgggcgccga
     5161 cgccgtgatt tactctcgcg tgagttgccc gatcatctcc gcccactccg gccagcccag
     5221 ggtcaatgct tccctcgctc ccgacgcata gtccgcatag tcaaaccccg ggctcactgt
     5281 gcatcccaac aacgcaaccg ttccgcccgg aatcaaccgc gttccctgcc acactccctg
     5341 cggcaccacc gtctgcagtc gttgcccctg cgccaggtcc tgcccgagga ccactcgctt
     5401 cccggtccca tccggccaaa gctgcaacat ctccactgcg tcgccgagat agaagtgaaa
     5461 cacctcatcc gacgcgagct tgtgcatctc ggaaaaattc cccggctcca gcagatagta
     5521 gatggctgtc cccgccgacc gagcacccct gtatcggcct gtcggcaacg cctccagcgg
     5581 aattgactcc tccgccctcc acgtttcccg aaaccagcca ccctcgcgcg gatgcggctc
     5641 cagccccagc atcctcttca attccgcagc gttgatcgta ttcgccatgc ccgcatccta
     5701 acctgggttc ccacaagcat ccgcgcgcgg ttccgttgac cactcctttc gccaaacacg
     5761 atcgctagct gaacgctgcc aacgaggggc ggaacattgt ctgcctctcc gttgtctatc
     5821 cgcacagaga cgcgagcaca accggctcga acgtctctca gccctcgctc tcttcggcag
     5881 ttcgattcct gcgctccggg ccctgagctc tgagccttgt tccggagggg acccatgttc
     5941 gaagacagca ccttcgcatc cgcgaatcgc atccacactc gcagctcccg ttacgcagca
     6001 ggctcgctct ttctgcaaac gtctctcctc gccatccttg tgctcattcc ctttctctat
     6061 cccaacgcgc tccctccgaa attcctgtca gtctccctga tcgcacccac gccgccgccg
     6121 gccgcgcccg tggtcgagcc ccaacacgcc atcgcctcca cttcccacac tgaactcctg
     6181 acctccacca tccaggcgcc gtccacgatc ccgacgcgta tcccccacat cgtcgattcc
     6241 ggacctccgg gggccattcc ctccagcgcc ttcgccaact ccggcacgaa cacgctcccg
     6301 ctcggacccg ccacgccttc agtgacaccc cgcgttcaga ccgccaaaca caccggccct
     6361 attcacatct cgcaaggcgt cgctgcgggg caactcatcg tccccatcca gcctcgctac
     6421 ccacccatcg cccgcgcgac gggcacccag ggcaccgtca tggtctctgc catcatctcc
     6481 accgacggtc gcagtgagag cctccgcgtc ctgagcggtc cgccgatgct gataccggca
     6541 gccgtcgacg cgatccgcca agcccgctat cggccatgga ctctcaatgg cgcacccgtt
     6601 gaggtggaga ccaccatcaa cgtcgtcttt tcccttggaa acgactgacg agtgaaggcg
     6661 agcgaaaggc gggccaggga cgagaggtga atcgagcgga cgagcaagtt caggacgatc
     6721 agagaatgga tggctgcccg agattcaacc gggaacgagg ctaactgcgc gatccttcac
     6781 tcgccttcga ctcgcttcct gctcgccttt cactcgtttt ttgctggtct ttcactcgcc
     6841 cttttccatc aggcttcccc attcgtgtga ctcgcatcac tctaatcgtc gcgattgttg
     6901 cgatacgatg aacagcgatg cgggtcctcg tttattcagc tccgtggtgc cgtgattgtc
     6961 gcgaggccaa acgcttcctc gaatcgcacc a
//
LOCUS       DASZYN010000217         4087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_136705, whole genome shotgun sequence.
ACCESSION   DASZYN010000217 DASZYN010000000
VERSION     DASZYN010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136705"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(528..2231)
                     /locus_tag="VHE33_05640"
     CDS             complement(528..2231)
                     /locus_tag="VHE33_05640"
                     /inference="COORDINATES: protein motif:HMM:NF021374.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96970.1"
                     /translation="MAGVETVQPPQSASATGFLSPLARRQYTTIAWVQFRILINSLRT
                     RRGNLEFGAQILVFIFFCLIAGGPAFGLGFGAWAAASHDTFNGIALELWVLLLSWQFF
                     AALAPAIGGQNPELSHLLRYPVSFGSWVVLYLVYGLAAPSTLIGIIWAIAIGIGIGVA
                     RPDLFLWTLLTLALFVFFNLLLARTILAWIERWLAQRRTREIVTAIFLFLALAAQVFN
                     PVYRQSANSHPFSGIDKQTVSRVGTRIMNVQRLLPPGLAVGSIHSMADHDPLYGFADL
                     LWLGCFTLAAGSLLGLRLQSESRGENLSEAPRRRAKTTGKLRRPTLDFSGPIAAVIEK
                     DLRYLLRSGPMLYNLAAPLVMVFVFGGAFRGGRLVSSIRIEYALPIGMVWAFLGLARI
                     ISNNLGMEGEGIQFFFLSPTPMRTVILGKNFLHLAIFSIEAILISAVVIYRFGWPVPS
                     VAAATLAWLLFAVPANFTIGNLLSINMPYRINMTRMRRETGAVGNNLTSFVTQLGILG
                     IGALVIAPCAFLGEPWLPVPIFLVLAAASFFLYWRVLGNVDRMVHARMDSLVLEVMKT
                     R"
     gene            complement(2219..3028)
                     /locus_tag="VHE33_05645"
     CDS             complement(2219..3028)
                     /locus_tag="VHE33_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT96971.1"
                     /translation="MDSPAISVQNLTRRFGSFTAVDNISLDVAAGQFFGFLGPNGAGK
                     STTIKMLTGLLAPTSGSIHLLGIDFSVHPLDVKRQIGVVPEGLALFGRLTASEYLRFA
                     GRMYGLDRETTNRRTEELLDFMNLADEPRKLVTDFSHGMQKKLALAAAVIHGPRILFL
                     DEPFEGVDAIAAGTLKAMLQRMTERGATIFLTSHVLEIVERLCSHVAIIHQGRIVANG
                     SLDELRAGVTAIPGELAEAGQKLTLEEIFLRIVSGSSAGAEEPLQELSWLA"
     gene            3215..>4087
                     /locus_tag="VHE33_05650"
     CDS             3215..>4087
                     /locus_tag="VHE33_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96972.1"
                     /translation="MPEIFAPGVISGPGNDGAPTFSPDGKTLYFTRSAAHWTAIVESH
                     EVNGQWSRPTLAPFSGEWPDSSPAMSPDGTFIVFQSTRPMAPLKEAPKPGVPIPGIVS
                     NLWRVDRTGSGWSAPVRLPDTVNIGRSIWKPSIAADGTIYFTNIDNKGGKRLYSARFV
                     QGSYEMAQPLPFSDGTKADVDPEIAPDGSFLVFCSAGRLGDTNKDQLFLVRRNGAGWG
                     EVERIRYEGDEKPYGPSTDDEPRLGPDHRTLYFSSDRAVVVHFPRTREMAEKDLERLD
                     SWDNSNNNVWSISLT"
BASE COUNT          844 a          951 c         1577 g          715 t
ORIGIN      
        1 gggggcaacg aatcctgcga atgcagagga ggggacgatc cggaagaagt atgcggggtc
       61 gatcgaggag aatgcgattc acgggtcgga cgccgaggag acggcgcagt tcgagattgg
      121 gtatttcttc gccgggcatg agctggtgta gttcgccgcc agtcgggaag caaaaggacc
      181 ggggcatagg ctccggtcct tttgcttttg tgctgggcgg ttggcccaga gttgtttccc
      241 gatttgagat gcgaacggta ctttcgcgtg gagcgagagc tcggatttct tgatttgcaa
      301 aggctggagg acgaagcttc ttgctgtggg gtgaacttgg ccgtctcaat gcggctgtcg
      361 gcgccccgtc gcttccgctt cccgattcgg gacgcgaacg gtactttcgc gcggaaaagc
      421 ggcccggatt tcttgattag cgagggctgg agggcgaagc ttcttgctct ggggtgaacc
      481 tggccgtctc aatacggcgg gttttgtacg gcggtttcgg acggttccta gcgagtcttc
      541 atgacttcca gaaccagcga gtccatgcgg gcgtgcacca tgcggtccac gttgccgagc
      601 acgcgccaat agaggaaaaa gctcgcagct gccaggacga gaaagatggg aacgggcagc
      661 cacggttcgc cgagaaaggc acagggtgcg atgaccagag cgccgatgcc gaggatcccg
      721 agctgcgtaa caaagctggt gaggttgttg cccacggcgc cggtttcgcg gcgcatgcgg
      781 gtcatattga tgcggtaggg catgttgatg gaaagcagat tgccgattgt aaagttcgca
      841 ggaacagcaa agagcagcca ggccagggtc gcggcggcaa cgctggggac cggccatcca
      901 aagcgataga tcaccacggc ggagatgagg atcgcctcga tcgagaagat ggcgagatgg
      961 aggaagttct tgccgaggat aacggtgcgc atgggagtgg gcgacagaaa gaagaactga
     1021 atgccctcac cttccatacc gaggttgttg gagataatgc gggcgagacc gaggaaggcc
     1081 cagaccatgc cgatggggag ggcatattcg atgcgaatgc tgctgacgag ccgacctccg
     1141 cgaaaggcac ctccgaagac gaagaccatg acgagcggag cggcgaggtt gtaaagcata
     1201 gggccgctgc gcaggaggta gcggaggtct ttttcgatga cggcggcgat gggtccggag
     1261 aagtcgagcg tcgggcggcg gagtttaccg gttgtctttg cgcggcggcg gggggcttcg
     1321 ctgaggtttt cgccgcgact ctcggactgg aggcgcagac cgaggagact gccagcagcg
     1381 agagtaaaac agccgagcca gagcagatcg gcaaaaccat aaagaggatc gtgatccgcc
     1441 atggagtgga tggagccgac ggcgaggccg ggtgggagga ggcgctgcac gttcatgatg
     1501 cgggttccaa cgcgactgac ggtttgcttg tcgatgccgg agaagggatg gctgttggcg
     1561 ctctggcggt agaccgggtt aaagacctgg gcagcaagag cgaggaagag aaagatggcg
     1621 gtgacgattt cgcgcgtgcg gcgctgcgcg agccagcgct cgatccaggc aaggatggtg
     1681 cgggcgagga ggaggttgaa gaagacgaag agagcgaggg tgaggagggt ccaaaggaag
     1741 aggtcggggc gggcgacgcc gatgccgatg ccgatggcaa tggcccaaat gatgccgatg
     1801 agggtggagg gggcggcaag tccgtagacg aggtagagga cgacccagga accgaagctg
     1861 acgggatagc gaaggaggtg gctgagttcg ggattttggc cgccgatggc gggagcgaga
     1921 gcggcgaaga actgccagga gagcagaagg acccagagct cgagcgcgat tccgttgaag
     1981 gtgtcgtggg aggccgcggc ccaggcgcca aaaccgagac cgaaggcggg gccgccggcg
     2041 atgagacaga agaagatgaa gacgaggatc tgtgcgccga attcgagatt gccgcggcgg
     2101 gtgcgcagag aattgatcag gatgcggaac tggacccagg cgatggtggt gtattgccgg
     2161 cgggcgaggg gtgagaggaa ccctgtggcg ctggcgctct gcgggggctg aacggtttct
     2221 acgccagcca tgaaagctcc tggaggggtt cttcggctcc tgcagaagag ccactgacga
     2281 tgcggaggaa gatttcctcg agggtgagtt tttgtccggc ttcggctagc tcgccgggga
     2341 tggcggtaac accggcacgc agttcatcga gcgaaccgtt ggcaacgatg cggccctgat
     2401 gaatgatggc gacgtggctg cagaggcgct cgacgatttc gaggacatgc gacgtgagga
     2461 agatggtggc gccgcgttcg gtcatgcgct ggagcatggc cttaagggtg ccggcagcga
     2521 tggcgtcgac gccttcaaag ggctcgtcga ggaagagaat gcgggggccg tggatgacgg
     2581 ctgcggcgag ggcgagcttc ttctgcatgc cgtgggagaa gtcggtgacg agcttcctgg
     2641 gctcgtcggc gagattcatg aagtcgagga gttcctcggt gcgccggttg gtggtttcgc
     2701 ggtcgaggcc gtacatgcgg ccggcgaagc gaaggtattc ggaggcggtg aggcggccga
     2761 agagggcgag gccttcgggc acgacgccga tctgacgctt gacgtcgagg gggtgaaccg
     2821 aaaaatcgat accgaggagg tgaatgctgc cggaagtggg ggcgaggagt ccggtaagca
     2881 ttttgatggt ggtggatttg ccggcaccgt tggggccgag gaatccgaag aactggccag
     2941 ccgcgacatc gagggagatg ttatcgaccg cggtgaagga gccgaagcgg cgggtgaggt
     3001 tttggacgga gatggcggga gaatccattg gaggtagggt acagcgtaca gggggcgggg
     3061 cgcaggggca cgtggggtgt gcagggtgga gttgctttcc tcctcaacgg ttgtaagtcc
     3121 aagagtgaac gaggtgcatg tgtcgaaaca aggatggatt tggctgtgtt gcggtttgct
     3181 ggcactgcag ctacaggggc aacagaaatc gcccgtgccg gagatttttg cgcctggggt
     3241 gatctcggga ccggggaacg atggagcgcc cacgttttcg ccggatggca agacgctcta
     3301 ctttacgcgg agtgcggcgc actggacggc gatcgtggag tcgcacgagg tgaatgggca
     3361 atggagccgg ccgacgctgg cgccgttttc gggggagtgg ccggattctt ctccggcgat
     3421 gtcgccggat ggaactttca tcgtatttca gtcgacgcgt ccgatggcgc cgttgaagga
     3481 agcgccgaaa ccgggcgtgc ccattccggg gatcgtttcg aatttgtggc gggtagatcg
     3541 aaccggatct ggctggagcg cgccggtgcg gctgccggat acggtgaaca ttgggcgatc
     3601 gatctggaag ccgagcatcg cggcggacgg aacgatttac ttcacgaaca tcgacaacaa
     3661 gggtgggaag cggctgtatt cggcgcggtt tgtgcagggc agctacgaga tggcgcaacc
     3721 gctgccgttc agcgatggga cgaaggcgga tgtggatccg gagatcgcgc cggatgggtc
     3781 gttcctggtg ttctgcagcg cggggcggct gggcgacacg aataaggatc agctgttcct
     3841 tgtacggcgc aacggagcgg gatggggaga ggtagagcgg atccgctacg aaggagacga
     3901 gaaaccgtac gggccgagca cggatgacga gccgcggctg gggcccgatc acaggacgct
     3961 gtatttcagc agcgatcggg cggtagtggt gcattttcct cggacgcggg agatggcgga
     4021 gaaggatctg gagcggctgg acagctggga caacagcaat aacaacgtgt ggtcgatttc
     4081 gttgacg
//
LOCUS       DASZYN010000218         3450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_843777, whole genome shotgun sequence.
ACCESSION   DASZYN010000218 DASZYN010000000
VERSION     DASZYN010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843777"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VHE33_05655"
     CDS             complement(<1..239)
                     /locus_tag="VHE33_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96973.1"
                     /translation="MLRSLACAAILAAAIAAASVPQAAGQARPAGDAAVSPIPNTPPA
                     AAEKSGPDTEKDDAKHPASKHDRAQAAKLFLDGAK"
     gene            complement(229..996)
                     /locus_tag="VHE33_05660"
     CDS             complement(229..996)
                     /locus_tag="VHE33_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVT96974.1"
                     /translation="MSTTPEARVYVKEGFRWDEQRAYLFDIDGTLMRHRDRVHVDAFF
                     RGVRDVMGRDLVLDGVVLSGNTDPGILRDAFALASIDDSVWRPHREDVLRAMREHVAE
                     RRAEMLWTVMPGVEAMLEHLEGKGALLGVATGNLEEIGWIKIENAGLRRWFRFGGFCD
                     RFEARQDMIAHAAEQARAILQSTDGPGDTATMCVIGDTPRDIEAARANGLATIAVATG
                     RYTFDQLMEYEPEGCTTTLEALLEASGLSAKAESHAS"
     gene            complement(1192..1476)
                     /locus_tag="VHE33_05665"
     CDS             complement(1192..1476)
                     /locus_tag="VHE33_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVT96975.1"
                     /translation="MKRELDNLITQMHAGGITYDEAVREFKKRFILEVLSSNRGNQCK
                     AAKELGVHRNTLSRILTDLKIDPAQVRTAQRRPPQRERGVFDGSRRGERA"
     gene            complement(1544..2080)
                     /locus_tag="VHE33_05670"
     CDS             complement(1544..2080)
                     /locus_tag="VHE33_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HVT96976.1"
                     /translation="MAIIDWVILIVVILSVVSAAKAGLVLEVCNLAGLILGLLVASWD
                     YQKLTPWMGQWVHSIALNETLSFITIALGVMLVAGIVGRLIRWSVKSVGLGWMDRLAG
                     AAFGLLKGCALVTVAVMVIAAFWPNATWFRQSRFAPEFLGMAHTAAAVAPAELGDRIR
                     SGVVVLRKAQPAWMKPAA"
     gene            complement(2092..3051)
                     /locus_tag="VHE33_05675"
     CDS             complement(2092..3051)
                     /locus_tag="VHE33_05675"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HVT96977.1"
                     /translation="MVVCASEDGSRLDAKPQERPVELKALLETDFRNLTLVARGKVRD
                     IYALGEYLLMVATDRISAFDHVLATGIPGKGMILTQLSLFWFDFLKDIVPNHLVASNV
                     KEFPEEVWKYRKELKDRSMLVTRAQMYPVECVVRGYLAGSGWKDYVATGTVCGIKIEP
                     GLREGDRLSRPIFTPATKSFRGTHDENIAFDQMVTLIGGDAAEKLRDLSLALYNKAAA
                     HAESRGILLADTKFEFGSTSMGIVLADEALTPDSSRFWVKELYSPGRSQPSFDKQYVR
                     DYLESIHWNKLAPAPALPENVVNRTQQKYLDAYRLLSGRPLAV"
BASE COUNT          662 a         1097 c         1016 g          675 t
ORIGIN      
        1 ttctttgcgc cgtcgaggaa gagtttcgcg gcctgggcgc gatcgtgctt ggaggcggga
       61 tgtttcgcat cgtctttttc cgtgtcgggg cctgactttt cggccgcggc gggcggggtg
      121 ttcgggatcg gcgatacggc cgcgtcgccg gctggtcttg cctggcctgc cgcctgaggc
      181 actgacgccg cggcgatcgc ggctgccagg atcgctgcgc aggcgaggct acgaagcatg
      241 gctctccgcc ttcgcagaca gtcccgacgc ctcgagcagc gcttccaggg tggtggtgca
      301 cccctcgggc tcgtactcca tgagctggtc gaaagtgtat cggccggtgg ccactgcgat
      361 ggtcgcaagg ccgttggctc gcgccgcttc gatgtcccgc ggagtatcgc cgatgacgca
      421 catcgtcgcc gtatctccag ggccatcggt ggactggagg atcgcccgcg cctgctcggc
      481 ggcgtgcgcg atcatatcct ggcgcgcctc gaagcgatcg cagaagccac cgaaacggaa
      541 ccagcggcgg agcccggcat tctcaatctt gatccagcca atctcttcca gatttccggt
      601 tgctacaccc aggagcgcac ccttgccttc gagatgttcc agcatcgcct ccacgccggg
      661 catgacggtc cacagcatct ccgcgcggcg ttcggccaca tgctcgcgca tggcgcgcag
      721 aacgtcctcg cgatgcggcc gccacacgct gtcgtcgatg ctggccagcg cgaacgcatc
      781 gcggaggata ccgggatcgg tattgcccga cagcacgact ccatccagca ccagatcccg
      841 gcccatgacg tcgcggactc ccctgaaaaa ggcgtccacg tgaacccggt cgcgatggcg
      901 catcagcgtt ccgtcaatgt cgaaaaggta ggcccgctgt tcgtcccagc ggaaaccttc
      961 cttcacgtag acgcgcgcct cgggcgttgt gctcatgctt acagggtata aggcggggac
     1021 ggatcacgtc acatcgccgc cggtctgccc tcccgacgcg ccttcccgag gacacaaaag
     1081 gctcgcggtc agcgcgcctc gggtgggttg cgattcgtgc gggcgatgag attcttcgat
     1141 tttctcgatg gattccgaga gggctgcgga ttgccagccg gccgtctgtt actacgcgcg
     1201 ctccccgcgc cggctgccat caaagacgcc gcgttcgcgc tgaggcggac gccgttgcgc
     1261 cgtgcggacc tgcgccgggt cgatcttcag atcggtgaga atacggctga gggtgttgcg
     1321 gtggactccc agttctttcg ccgctttgca ctggttgccg cgattgctgg acaggacctc
     1381 cagaatgaag cgcttcttaa attcgcgcac ggcttcgtcg taggtgatgc cgcctgcgtg
     1441 catctgggtg atcaggttgt ccaactcacg tttcacagga acctctttcc cgatcccggt
     1501 cgatgccggg gcaatctctc aaatatcgta cccagcccaa accctaagcc gccggtttca
     1561 tccacgccgg ctgcgccttc cgcaggacga ctacaccgct cctgatccga tctcccagtt
     1621 cggcaggagc cactgccgcg gcagtatgcg ccatgcccag gaactccgga gcaaagcgtg
     1681 actgccggaa ccacgtcgca ttgggccaga aagcggcaat gaccatgacg gcaacggtca
     1741 ccagcgcgca tcctttcagc agaccgaagg cagcgcctgc cagtcgatcc atccagccca
     1801 gcccaaccga cttcaccgac cagcggatga gccttcccac aatgccggcc acaagcatca
     1861 cacccagcgc gatcgtgatg aacgagagcg tctcgttgag cgcgatcgag tgaacccact
     1921 gacccatcca gggcgtcagc ttctggtaat cccaactggc caccagcaat cccagaatca
     1981 gccctgccag gttacaaact tcgagcacca gtcccgcctt cgcagccgac acgaccgaaa
     2041 gaatcaccac gatcaggatc acccagtcga tgatggccat agcctgactc actagaccgc
     2101 cagcggcctg cccgacaaca aacgatacgc atccagatat ttctgctggg tgcggttcac
     2161 cacattttcg gggagggccg gggccggagc cagtttattc cagtgaatgc tctccagata
     2221 gtcacgaacg tactgcttat cgaacgaagg ctgcgaacgg cccggcgaat acaactcttt
     2281 aacccagaag cgcgatgagt ccggcgtgag cgcctcgtcg gcgagcacaa tgcccatcga
     2341 agtggaccca aattcgaatt tggtgtccgc cagcagaata ccgcgagatt cagcgtgtgc
     2401 cgcagctttg ttataaagcg ccagactgag gtcacgaagc ttctccgccg cgtcgcctcc
     2461 gatgagtgtg accatctggt cgaaggcgat gttctcgtcg tgcgtgccgc gaaagctctt
     2521 cgtggcgggc gtgaagatgg gacggctcag gcggtcgccc tcgcgcaaac ccggctcgat
     2581 tttgattccg cacaccgttc cggtagccac gtagtccttc cagcccgaac cggcgaggta
     2641 tccgcgcacc acgcactcca ccggatacat ctgcgcccgg gtcaccagca tcgaccggtc
     2701 cttcaattct ttgcggtact tccaaacttc ctctggaaac tcctttacgt tggaagcgac
     2761 cagatggttc ggaacaatgt ccttcagaaa atcgaaccag aagagggaga gctgggtgag
     2821 gatcattccc ttgccgggga ttccggtggc cagcacatga tcgaaagcgg agatgcggtc
     2881 ggtggcgacc atcagcaggt attcaccgag cgcataaatg tcgcgaactt tgccgcgggc
     2941 caccagagtg aggttgcgga agtcggtttc gagcagcgct ttcaattcaa caggcctttc
     3001 ctgcggcttc gcatcgagtc gtgagccgtc ttcgcttgca cagaccacca tcgtcgaggc
     3061 tgttgccgga gggagtgcgt gggtcgagcg accagtcccg gagggagacg atggaatcaa
     3121 gctgaaatcc gattgtgcga ttcactctgc gctttgtcaa cgctgtgtga aggcgttcat
     3181 tttcgcgctt tcggcgcgat ttaactccgc ttctcaagca tgtcaagagg aaagtcgttg
     3241 ctatcgcaac agcgtttcat ccagcggaac acgagcccat cagcgagcgg cgtttggcga
     3301 acagcgttta gcgaaaagct gttcgcgagg agcggtgagc cagacaggag ttcttcgccg
     3361 aagtcctttg caacgtatcc cggtaaggcc cttcgctctg cattttcctg ccgagagcaa
     3421 cttccaaatg gtcaggcgct caacgctcta
//
LOCUS       DASZYN010000219         4337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_458828, whole genome shotgun sequence.
ACCESSION   DASZYN010000219 DASZYN010000000
VERSION     DASZYN010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458828"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1622)
                     /locus_tag="VHE33_05680"
     CDS             complement(<1..1622)
                     /locus_tag="VHE33_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HVT96978.1"
                     /translation="MSPRLALLWAAVSVLLAFPASAQVTFSTTTYSSNNLWNQNGPPN
                     TQLRVDLNGDGREDFVSQNTGSFNSGCTGSFAVALSTGDGAYAPPVCYTLPAGYAIDF
                     ATGDFYGVGTLDVAVSNETGDLYIYRNSGNGTLTLASSLALGAEAGGLVASDVNHDGH
                     IDLVYDIGNQNTGGGGTLLTLLGNGDGTFSAGPTTTFTMADEPAGSLSVGDFDGDGKG
                     DIMVVGQTLYNATVLYGDNTGNFTPGPVLGGPILGVSSASLTWYQGFDVNSDGIMDLI
                     GSPVTYTFCGTGCYPTVTENNYLDLERGQYGRTLSSERIPLQHCPSSANPPQVADFDG
                     DGNADIVLQEDADCKGSAPYTLDFMKGNGDGTFQPEQVIYSTNDSIGNWHVLRASHSS
                     KPDLTVWQYQDVNNVITNPEALVLVNTTTGNFPACTPLDYRPAGISVCGPTSTVGVTS
                     PVNFSFAGTNETPGRGMEIWVDGQKVAENLKQGYSYHDFIDASVPLGNGQHHVDVVSV
                     GWDYSLLVDSFPLTVGDDQCPIPQGGGLNACSP"
     gene            1999..2322
                     /gene="rplU"
                     /locus_tag="VHE33_05685"
     CDS             1999..2322
                     /gene="rplU"
                     /locus_tag="VHE33_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HVT96979.1"
                     /translation="MYAVIRTGGKQYRVAPGDVVKVESLTPGNNGSVEFADVLAFSGE
                     SGSVVKPQSAKVLGTVVSEGRADKILVFHFKRKKQYKKLQGHRQGYTQVRISEIQVDG
                     NSYTA"
     gene            2373..2636
                     /gene="rpmA"
                     /locus_tag="VHE33_05690"
     CDS             2373..2636
                     /gene="rpmA"
                     /locus_tag="VHE33_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HVT96980.1"
                     /translation="MAHKKGLGSSKNGRDSNAQRLGVKIFAGQVVTGGSIIVRQRGTR
                     LKPGLNVGRGTDDTLFAKIAGRVKFIDRGARGRFVAVEPVAAE"
     gene            complement(2788..3681)
                     /locus_tag="VHE33_05695"
     CDS             complement(2788..3681)
                     /locus_tag="VHE33_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96981.1"
                     /translation="MVHLQELLIRLEPILVVLVAFSFFKSRSAQRLPAMATYLVVRLA
                     SLAYLEIVYNYGGGIAATDTLRYRAYFYGYWITYLAVTVSIFFVTQEVFKRVMEPVPG
                     LRRLGLLAFRWISVVSIVVAVGAIALPNALSEQHAQNADKIGQICVQTVRCVSVMEIS
                     LLAFLALSIHSLGRSFRSRLFGLGLGFGLQAFADLVSAAIPVRCPGLISAADLLDQVA
                     TTAVLLTWAVYFLLPEPEAERNMIVLPPSSMLARWNALAKGLGQMPEPVMVGAPTGFF
                     LQDIEGVVDRVLAKNPVVAPR"
     gene            3814..4101
                     /locus_tag="VHE33_05700"
     CDS             3814..4101
                     /locus_tag="VHE33_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96982.1"
                     /translation="MRPVPLVPVQLAPGLQRGIGIAATSPVPLVPVQLAPGLQRGMGI
                     AATSPVPLVPVQLAPGLQRGIGIAATSPVPLVPVQLAPGLQRGIGIAATIA"
BASE COUNT          868 a         1302 c         1343 g          824 t
ORIGIN      
        1 ggactgcacg cattgagccc tccgccctgc ggtatcggac actggtcgtc gcccaccgtc
       61 agcgggaacg aatcgaccag cagcgaatag tcccagccca ccgaaaccac atccacatgg
      121 tgctggccgt tccccagcgg cacgctggca tcgatgaagt cgtgatagct gtagccctgc
      181 ttcaggttct cggctacctt ctggccgtcc acccagatct ccatgccgcg tcccggcgtt
      241 tcgttcgtcc ccgcaaagct gaagttgacc ggcgatgtga cgccgacggt ggacgtcggt
      301 ccgcacacgc tgatccccgc gggtcggtaa tcgagaggcg tgcaggctgg gaagttcccc
      361 gtcgtggtgt tgaccagcac gagggcctcg ggattggtga tgacgttgtt cacatcctgg
      421 tattgccaga cggtgagatc gggcttgctg gagtggctgg cgcggaggac gtgccagttc
      481 ccaatcgagt cgttggtgga gtagatgacc tgctcgggct ggaaagtgcc gtcgccgttg
      541 cccttcatga agtcaagcgt gtacggagcg ctccccttac aatccgcgtc ttcctgaaga
      601 acgatgtcgg cgttgccatc gccatcgaaa tcggccacct ggggtggatt cgccgagctc
      661 gggcagtgtt gcaacggaat tctctcactg ctgagcgtac ggccgtattg accccgctcc
      721 agatcgagat agttgttctc tgtgacggtc ggatagcagc cggtgccaca gaaggtatac
      781 gtgaccggcg agccaatcag gtccatgatg ccgtcggaat tgacgtcaaa gccctggtac
      841 caggtgaggc tggcgctgct gacgccgagg atcgggcctc cgagaaccgg gccgggagtg
      901 aagttgcccg tgttgtcgcc atagagaact gtggcgttgt acagcgtttg tcccaccacc
      961 atgatgtcgc ccttgccgtc gccgtcgaag tcgccgaccg acagcgatcc cgccggctca
     1021 tcggccatgg tgaacgtggt cgtcgggccg gcgctgaacg tcccatcgcc gttgccgagc
     1081 agggtgagga gagtccctcc tcccccggta ttctgattgc cgatgtcgta aacaaggtcg
     1141 atatgcccgt cgtggttcac atcggacgcg accagaccgc ccgcctcggc ccccagcgcc
     1201 aggctcgagg ccagcgtcag cgtgccgttg ccggagttcc ggtaaatgta gaggtcgccg
     1261 gtttcgttgc tcacggcaac atcgagagtg ccgactccat agaagtctcc ggtggcaaag
     1321 tcgatggcat aaccggcggg aagcgtgtaa cagacgggcg gcgcataggc gccgtcgccg
     1381 gtggacagcg ccacagcaaa cgagccggtg catccgctgt tgaacgagcc cgtattttgc
     1441 gagacgaagt cttcgcgccc gtcgccgttc agatcgacac gcagctgcgt gttcggcggg
     1501 ccgttctgat tccacaaatt gttgctggaa tacgttgtgg tcgagaaggt gacttgtgcg
     1561 gaagctggga aggccaacaa tacggacact gcggcccaaa gcaaagccag gcggggggac
     1621 atggactgga ctcctgaaga gttttgcgat cggtcgggcg aagggccgca cgcaccgaag
     1681 agtcgtgttg gcgcatctcc cggttcgtgg cttactgagg catcgtgcaa cttctaggat
     1741 ggccgcctga acggccaggg ttgcgctttg aggcgcctcc cggttgcggt ctctgcggta
     1801 acgcccaccg cgctgcattg acccgccgcg cattttcccg ataaactaac aaggtttggc
     1861 gattctgcct gtcccgcgtg tctccgggac tcttcggttg atttccgccg gagtcgaagc
     1921 acagtttccg cccagctcag tttgatacgc gcccgcagtt tccggtggtg tgttcgccgt
     1981 ccgggcgccg gaggaattat gtacgcggtc atccgcactg gtggtaagca gtatcgagtc
     2041 gctcccggcg acgtggtgaa ggtcgaaagc ctcacgcccg gaaacaacgg ttcggttgag
     2101 tttgccgacg ttctcgcctt ctccggcgaa tcaggctccg tggtcaagcc gcagtccgcc
     2161 aaggtactcg gcaccgtggt ctcggaaggg cgggcggaca agatcctggt cttccacttc
     2221 aagcgcaaga agcaatacaa gaagctccag gggcatcgcc agggttacac ccaggtgcgc
     2281 atcagcgaga ttcaggtcga cggcaacagc tacaccgcct aagcgcgcgt agcctccaac
     2341 tcccgcccag cgcggattca aggttctgga atatggcaca caaaaaaggt ttaggcagct
     2401 ccaagaacgg ccgcgactcc aatgcacagc ggctgggcgt gaagatcttt gccggccagg
     2461 tggtcactgg cggatccatc atcgttcgtc agcgcggaac ccggctcaag cccggcctca
     2521 atgttggccg cggcaccgac gatacgctct tcgcaaaaat tgccggtcgc gtgaagttca
     2581 tcgaccgggg cgcgcgcggc cgctttgtcg ccgtcgaacc ggtcgctgcg gagtagattc
     2641 gcggcgaacg caacatcgat ggccggctcc tccgggagcc ggccattttt cgtgttgcga
     2701 agaccgacat ttctccggca cggcttctcg cgggcaaagc aaaagccccg agaatgtctc
     2761 ggggctttta cgtgaagaag aagaccgcta tcggggagcg accaccgggt tcttcgccag
     2821 aacccgatcg accacgcctt cgatgtcctg caggaagaag ccagtcggcg cacccaccat
     2881 caccggctcg ggcatctgtc ccaaaccctt ggcgagagca ttccagcggg ccagcatcga
     2941 gctgggcggc aggacaatca tgttgcgctc cgcttccggc tccggcagca ggaagtacac
     3001 agcccacgtc agcagcacgg ctgtggtggc cacctgatcg agcagatcgg ccgccgagat
     3061 cagtcctggg cagcgaacgg ggatcgcagc ggaaaccaga tccgcgaacg cctgaagtcc
     3121 gaatcccagc ccgagcccga agagccggct gcggaaggaa cggcccagag aatgaatgga
     3181 aagcgcgagg aacgcgagca gtgagatttc catcacgctg acgcagcgca cggtttgtac
     3241 gcagatctgg ccaatcttgt cggcgttctg agcgtgttgt tcggacagag cgttcggcaa
     3301 agcgatcgct ccgaccgcga ctacgatcga aacgacgctg atccagcgaa aggccaggag
     3361 gcccagacgg cgcagacccg ggacaggttc catcacccgc ttgaagactt cctgagtcac
     3421 gaaaaagatc gagacggtga cagcgaggta ggtgatccag tagccgtaaa agtaagcccg
     3481 gtagcggagg gtgtccgtgg ctgcgatccc gccgccgtag ttgtagacaa tctcgagata
     3541 ggcgagggaa gcgagccgaa cgaccaggta cgttgccatt gccggcaaac gctgcgccga
     3601 tctggatttg aaaaacgaaa aggccaccag cactacaagg atcggctcaa gtcgtatgag
     3661 tagctcctgc agatgcacca tcgctttcct ctcaggcctt gaggatagcg aagtaacatg
     3721 accgactcaa gcgaaaacct ctagaaaaca aagaaccgcg ctgccacttt cgtggtagcg
     3781 cggttaccgt ggttcctgtc gaatctttaa gctttgcggc ccgttccgtt ggtcccggtg
     3841 cagttggctc cggggctgca acgcggcatc ggcatcgctg ctaccagccc agtgccgttg
     3901 gtcccggtgc aattggctcc agggctgcaa cgcggcatgg gcatcgctgc caccagtcca
     3961 gtgccgttgg tcccggtgca gttggctcca gggctgcaac gcggcatcgg catcgctgcc
     4021 accagcccag tgccgttggt cccggtgcag ttggctccgg ggctgcaacg cggcatcggc
     4081 atcgctgcca caatcgcctg atggctcgag ttcattggaa cagagccgac gtgcgaggtg
     4141 gcaaatgctg ctgtggcacc cgcgatacac aatccgagaa ttagggtctt gacggccttc
     4201 atgactgaag tactcctcaa caaaagttgt atgactctgc ccaccgctgt ggtacagact
     4261 ccgccataca aagaggacta tagctgttaa catgcccctg aatatttccc cggcggtatt
     4321 atctaagtat taaatgt
//
LOCUS       DASZYN010000220        12511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_458977, whole genome shotgun sequence.
ACCESSION   DASZYN010000220 DASZYN010000000
VERSION     DASZYN010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 12511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12511
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458977"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VHE33_05705"
     CDS             complement(<1..297)
                     /locus_tag="VHE33_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96983.1"
                     /translation="MLKELYGKWMVRWETALTTRDENRVVRPLEWGFEWLQDFIDAAG
                     LRALVERAGRDGRAAEAAMIAVNEAIVRDSEAFFGYERPTDFRLEERAPQLFPTN"
     gene            complement(297..1466)
                     /gene="alr"
                     /locus_tag="VHE33_05710"
     CDS             complement(297..1466)
                     /gene="alr"
                     /locus_tag="VHE33_05710"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HVT96984.1"
                     /translation="MTSTRPTWAEVSRSNLIHNHGVLRRLAGPETELLCVVKANAYGH
                     GLEACAQALESNGARWFGVTCVDEGIRLRRVLADAPILIMSGIWKGEASAALENDLTP
                     VVWDAQHLAQLEAAARRLRAAPGDCPVHLEIDTGMSRQGVQLKDLEPLLELFGQSSPL
                     RLQAVMTHFHTPGNAELRAGQVREFVTAIDRIVDGGLKPQFLSAGSSAAALEPDAELG
                     ELAKQTGAQLMVRAGISLYGYAPDAEHPSDGAAQLKPVMQWKTRVVSVREISAGASVG
                     YDATFTAKRASRIALLPVGYADGVNRLLSSRGSVLVRGQKAPVAGRVSMDHTTVDVTD
                     VPGVEVGDEVVLIGTQNSEQGAEKVTAADVARLTGTISYEVLCAVSARVPRTMVD"
     gene            complement(1688..2281)
                     /locus_tag="VHE33_05715"
     CDS             complement(1688..2281)
                     /locus_tag="VHE33_05715"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVT96985.1"
                     /translation="MAVCVCAMSAAPGVVRAQQADSVAPGQTQEPPAVKEFQALEDQW
                     SNAVVKNDQYTMEFLLSPAYVDIASNGDVMTRNQLIAGLFDKGGPEPVSMEQRVVSVR
                     EFGDTAIVSGTYIVKNRANGSTKEERGIFTHIYNRTQRHWACVNAQRTAVMELAANKQ
                     KATQKKSQADEPFHIPFFHKGEQPSQTATGPTGIPPN"
     gene            2459..3859
                     /gene="dnaB"
                     /locus_tag="VHE33_05720"
     CDS             2459..3859
                     /gene="dnaB"
                     /locus_tag="VHE33_05720"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HVT96986.1"
                     /translation="MATTPDIEFERGLPASLDAERTILGAILLDNQAYSEAATRLRPE
                     DFALTAHQRVYARMGELIDRNHVVDIVTLAEELSRRKEIESVGGVAWLASLTEGLPRR
                     ITIEEYVRIVKDKSLLRQLINICSTAITRAADQSEEALEVLNAAETSLLEVTEKGITR
                     GFTSLHEIVAGSFKSIDNLYAQGQHQVTGLRTFLTKFDDMTAGLQNSELIIIAARPSM
                     GKSALAINLAQNAAIKGGKVVAVFSLEMSKESLLRRMLASEAHVGAKLIQTGFIPRDE
                     REKLTNALERLVEARIFIDDTPGISLTEMRAKARRLRQSAGALDLIVVDYLQLMTAGS
                     FGGPEGRRYENRTQEVSAISRGLKALAKELEIPVVALSQLSRASEQRGGDRKPILSDL
                     RESGSIEQDADVVAFIHRESYYNREDPDLEHKAEIIIAKQRNGPTGSIDVGWVSDYTL
                     FADLDEYPGQDAGGDY"
     gene            complement(4763..6886)
                     /gene="mnmG"
                     /locus_tag="VHE33_05725"
     CDS             complement(4763..6886)
                     /gene="mnmG"
                     /locus_tag="VHE33_05725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HVT96987.1"
                     /translation="MKGYVPVPMVFTEQYDVVVVGAGHAGCEAAMAAARMGLRTALFT
                     LNLDLIAQMSCNPAIGGIAKGHLVREVDALGGIMGEVADAVGIQFRLLNTSRGPAVWS
                     PRAQCDKQQYRVKMREVLESQAGLFIKQAEVVDVVLDTMTSVQRSAISVQDDDRCSAF
                     GVPEDSDGGELPRVVGVKLRDGRVVYAGATVITTGTFLNGLIHCGEENYPAGRSGEPP
                     SVLLGEALKKLGLRGCRLKTGTPPRLDGRTIDWSKFAEQPGDADPTPFSFRTKTLPQR
                     QVSCHIAYTTPETLRIIRENVHRSPMYSGQITGIGPRYCPSIEDKVVKFPDKEQHQFF
                     LEPEGLNTHEVYVNGMSTSLPMEVQWQIVRSIPGLENAEMLRPGYAIEYDAIDPTELD
                     RGLRVKKFAGLYLAGQINGTSGYEEAACQGLMAGINAALSVKGEAAFTLDRTEAYTGI
                     LIDDLISKGTNEPYRMFTSRAEFRLHLRIDNADRRLTPHGRRLGLIGDDAWAEYEAKQ
                     ARAVALEKVLWTCRPGGSALRAQDSDAHGDADGGSGSWPAGQSWGQLLRRPEVTMETV
                     WPALREQLEDPVFAPWMEAGAEGRLGARAHNELKTVETEIKYAGYLDQQKKAIEKLKR
                     AEERGIPEWFDYTQVSGLSREMQEKLMRVRPATLGQASRIPGVTPAALSLVNVYIEIQ
                     GKRTSVQRSAFSVQQDPAASEEAVG"
     gene            complement(7259..10582)
                     /locus_tag="VHE33_05730"
     CDS             complement(7259..10582)
                     /locus_tag="VHE33_05730"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT96988.1"
                     /translation="MREICNRLCTLFLALVVLAFFATPVLHGQTAVDGAIGGTVMDSS
                     GAVVSGSAVTVRNVATNAQQTATTDSNGYYRIIHLQPGVYSVSISAPGFRDYKATDVT
                     VQVGSMTDVEGRMEVGTAAQTVEVTGEAPIINTTSADFAGVVDQVPLKDLPENNYRWS
                     AFALLTPGVTNDSNGYGLLSFRGASTLLNNVTIDGTDDNQAYFSEERGRTRAGYSTIK
                     DSVQEFQVNTSNYSSEYGRSAGGIVNSVTKSGTNAFHGDVYYFDRDSDWNAQNYYVQH
                     PVQTSPGHYTAVPFKPTDLRRQYGFTVGGPLLKNRLFFFLGADRFYHDFPGAATITGT
                     SANANFYTAPDATLPAGKVCGVTSGTTAPNYQDSNVCQFSTNTGLSYADAASYYSNGI
                     ASMTSMLGQVARTGDQTIYFPKIDWQINDKNRASVEANRMRWTSPAGIQTSPAVAYGM
                     SSFGNDYVRDNWIVGKLDTFLTPTFSNEVRYMYGRDFEFEFNQTPTSYEQNNLVKTAN
                     YTNSLGLPANVYLSGFFQFGTPQFLNRAALPDERRWQIADTLEWIHGNHDVRFGGDYI
                     HTYDLISNLYNQYGGFTYSGNTPLGNYLADLYLSQNPQPGKTAEHYTYFNQGAGLPGL
                     EFTSGDYSLFVQDEWKIRPRLSLTGGLRWEFEKMPDPMLANSLVPATSKMPSNKGNIG
                     PRVGFAYDVFGHGTTVVRGGYGFFFARAINATIYQNLIVTGASGSQTNPQFNPGAACA
                     PQFPQVVPPSSYGSCLLGASGNATVYFFDKNFRLPAIYQGDLSLQQQITKNDVFGLSW
                     LGSWGRRLPDFVDTNLPAPTQVSFTISDPGGKGPLADGTTLTTNAYLGKRPNTNFSSI
                     TDTFSGVTSNYEALVAQFQHRMSHYASFSASYTWSHALDYGENNNTAPTANALLDPAN
                     LKLDYGNSNQNVPNRLMMYSVLDSPWHVHGPLGYLANDFQLAPTFQFQSGLPYSMGIS
                     GSQSKLYLTPGATAQGSLVSTGSFNGSNGANRVPFVDRNVFNMPHTVGVDLRASKRFV
                     VKERYSLEFLAEAFNLANHQNVTGIGATAYSVALDNTNHVNQLTPYTSTPFQSVTSTD
                     NSNFAYNVRQLQMALRFQF"
     gene            complement(10936..>12511)
                     /locus_tag="VHE33_05735"
     CDS             complement(10936..>12511)
                     /locus_tag="VHE33_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96989.1"
                     /translation="VPDTNAALVNADIPETARLAHDRRGFGPRGGFALDVFGKGRTVL
                     RGGYGIYTARVPNAMVFSVLTTTGSARSPRTYSYRPLDVAAPAFPYVFVSDAKPYVNP
                     TAPNHNSTAPEAVYFDPHFRRPQIYSMEVSLQQELGFRTVLTLTGLATDGRFLTQFVD
                     KNIDLENWATVFYSVRAPGNEGAIGPLGKATSGVSGYTNQVYEPRKFYYQRLNPAYGA
                     ITAMVSETNSSYRGAVVQLTRRMSRTVTANAAYTWSHTIDDNQNAATFAERNDVYDPA
                     DLQMEHGTSDYDVRQRVAGGVVLRAPWRLRGPAGMLLGGYSLGATGAWRTGLPWSMRT
                     SGAVPTPSCSYANWLNAGGATGGGANCLKAVTQPNETFTDNTAGMAVPIASLGGSLNG
                     SGGEDLIPPIGRNTFRYPAAVSLDLRFTKTIRISDRCSFEVMGEAFNALNHRNVTNMQ
                     SIGYRETNDKSHANMATLTWQSGMKPGTKTVLVNGRTETQYVYDPTAAFGGVTNANNG
                     AVSRERQLQVGLRLRF"
BASE COUNT         2359 a         4577 c         3160 g         2415 t
ORIGIN      
        1 gttcgttgga aacagctgcg gcgctcgctc ctccagtcga aaatccgtcg gtcgttcgta
       61 tccaaaaaat gcctcgctgt cgcgtacgat cgcctcgttc accgcaatca tcgccgcctc
      121 agccgcgcgt ccatcccgtc ccgcgcgttc cacgagcgct cgcagcccag ccgcatcaat
      181 aaaatcctgg agccactcaa agccccactc cagcggccgc accacgcgat tctcatcccg
      241 cgtcgtcagc gccgtttccc agcggaccat ccacttcccg tacagctctt tcagcatcaa
      301 tccaccatcg tccgcggcac ccgcgcactc accgcgcaca gcacctcata gctgatcgtc
      361 cccgtcaggc gcgccacgtc cgccgccgtc accttctccg ctccctgctc cgaattctga
      421 gttccgatca gcacgacttc gtctcccacc tccacgcccg gcacatccgt tacatccacc
      481 gtcgtgtgat ccatcgagac tcgccccgcc actggcgctt tctgccctcg caccagcacc
      541 gacccccggc tcgacagcag ccggttcacc ccatccgcat agcccaccgg caacagcgcg
      601 atcctcgacg ccctcttcgc cgtgaacgtc gcatcatagc ccacgcttgc tcccgcagaa
      661 atttcccgca cgctcacaac ccgcgtcttc cactgcatca ccggcttcag ctgtgccgca
      721 ccatcgctcg gatgctccgc gtccggcgca tagccgtaga gactgatccc cgcccgcacc
      781 atcagctgtg cgcccgtctg cttcgctaac tcccccagtt cagcgtccgg ctccagcgcc
      841 gccgccgaac tccccgcgct caaaaactgc ggcttcagtc caccatccac aatcctatcg
      901 atcgctgtta caaattcgcg aacctgcccc gctcgcagtt ccgcgttccc cggcgtgtgg
      961 aaatgtgtca tcaccgcctg gagccgcaga ggcgagctct gcccaaaaag ttccagcagc
     1021 ggctccagat ccttcagttg aactccctgc ctcgacatcc ccgtatcgat ctccagatgt
     1081 acagggcaat cgcccggcgc cgcacgcagc cgccttgccg ccgcttccag ctgcgccagg
     1141 tgctgcgcat cccaaaccac cggcgtcaga tcgttctcca gcgcggccga agcctccccc
     1201 ttccagatcc cgctcatgat cagaatgggg gcatccgcaa gcacgcgacg caggcgtatg
     1261 ccctcatcga cgcacgtcac cccgaaccac cgcgctccat tactttccaa cgcctgcgcg
     1321 catgcctcaa ggccatgccc atacgcattc gccttcacca cacacagcaa ttccgtctcc
     1381 ggcccggcca gccggcgcag aacaccatgg ttgtggatca ggttgctgcg cgaaacctca
     1441 gcccacgtgg gtcgtgtgct cgtcatcgtc tccagcctgg cacatcgcga acaaggagta
     1501 gctaacggct agggctccga cccgatgatt cggtgccttt cgcgaagcgg tcccgcccga
     1561 gcgggacgac ggtagaagcc ccggccttca ggccggggaa acagcagcta gaaaatctcg
     1621 ggcctttagg cccggaaaac ttcacgtgat cacgccgcca gctcagctta tgcgcttcca
     1681 tttcccgcta attcggcgga atccccgtcg gccccgtcgc cgtctgcgac ggctgctctc
     1741 ccttgtggaa gaacgggata tggaacggct cgtccgcctg gctctttttc tgcgtcgcct
     1801 tctgcttgtt cgccgccagc tccattaccg ccgtccgctg cgcgttcaca catgcccagt
     1861 gtctctgtgt gcggttgtag atatgcgtga agatcccgcg ctcctctttc gtgctgccgt
     1921 tcgcgcgatt cttaacgatg tacgtcccac tcacaatcgc cgtatcgccg aactcccgca
     1981 cgctcaccac gcgctgctcc atgctcaccg gctccggacc gcccttgtcg aacagccccg
     2041 cgatcagctg gttccgcgtc atcacgtcgc cattcgacgc gatgtccaca tacgccggcg
     2101 acagaagaaa ctccatcgtg tactggtcgt tcttcaccac cgcgttgctc cactgatctt
     2161 ccagcgcctg gaactccttc accgccggag gctcctgcgt ctgacccggc gcaaccgaat
     2221 ccgcctgctg cgcccgcact acgcccggcg ccgcgctcat cgcgcacaca caaactgcca
     2281 tcaaccaatt ccgaaaatgc atagataaat ccgctctcgg gagaactccc tccatgatag
     2341 acgttcagcc acccccgaag ccatcgtcat tcccctctgt gaaccccttt ttcacagaac
     2401 gttcctgatt cctcacagcg cccgcgaccc cctttccgtc ctaaactcat tcaggatcat
     2461 ggctaccacc cccgatatcg aattcgagcg cggcctcccc gcttccctcg acgccgaacg
     2521 caccatcctc ggcgccatcc tgctcgacaa ccaggcctac agcgaagccg ccacccggct
     2581 ccgccccgag gatttcgccc tcaccgctca tcagcgcgtg tacgcccgca tgggcgagct
     2641 catcgaccgc aatcacgtcg tcgacatcgt caccctcgct gaagagctct cccgccgcaa
     2701 ggaaatcgaa tccgtcggcg gcgtcgcctg gctcgcctcc ctcaccgaag gcctcccccg
     2761 ccgcatcacc atcgaggaat atgtccgcat cgtcaaggac aagtccctcc tccgccagct
     2821 catcaatatc tgttccaccg ccatcacccg cgccgccgac cagagcgaag aagccctcga
     2881 agtcctcaac gctgccgaaa cctccctcct cgaagtcacc gagaaaggca tcacccgcgg
     2941 cttcaccagc cttcacgaaa tcgtcgccgg ctcgttcaaa tcgatcgata acctttacgc
     3001 ccagggtcag caccaggtca ccggtctccg taccttcctg accaaattcg acgacatgac
     3061 cgccggcctc cagaattccg agctcatcat catcgccgcc cgcccgtcca tgggcaaaag
     3121 cgcgctcgcc atcaacctcg cccagaatgc cgccatcaaa ggcggcaaag tcgtcgccgt
     3181 cttctccctc gaaatgtcga aggagtcgct cctccgccgc atgcttgcct ccgaagctca
     3241 cgtcggcgcc aaactgatcc agaccggatt cattccccgc gacgagcgcg agaaactcac
     3301 caacgccctc gagcgcctcg tcgaagcgcg gatctttatc gacgacaccc ccggtatttc
     3361 cctcaccgaa atgcgcgcca aggcccgaag gctgcgccag tccgccggtg ccctcgacct
     3421 catcgtcgtc gactatctgc agctcatgac cgccggctca tttggcggcc cggaagggcg
     3481 tcgctacgag aaccggaccc aggaagtatc ggccatctcg cgcggtctca aagcgctggc
     3541 caaggaactt gagatccccg tggtcgcgct ttctcagctc tcccgcgcct ccgagcagcg
     3601 cggcggcgat cgcaaaccca tcctctccga cctccgcgaa tcgggctcca tcgaacagga
     3661 tgccgacgtc gtcgccttca tccaccgcga gagctactac aaccgcgaag atcccgacct
     3721 cgagcacaaa gccgagatca tcattgccaa acaacgcaac ggcccaacgg gctccatcga
     3781 cgtaggctgg gtctccgact acaccctctt cgccgactta gacgaatacc ccggccagga
     3841 cgccggcggc gactactagc gaagcaaggg aggccccgcg gctttgccgg ggaggcagtc
     3901 aaagttcgac gtttccggaa gttgaatttg tttttgcagt ttcggatgcc ggtcgcatcg
     3961 gagtgtctgc cgttgccgtt gtttttgctt ttaccggttg cgatcaggcc cctttgaggg
     4021 ccagcaaccg taaagcctcc ggctttgccg gaggatactt acttgagagc gatcaatgag
     4081 tcagcggcgg cggccagcga atcagcgact aaatccgccc gacgccaagt tcgagttcta
     4141 acttccgggt gcacatcctg gtcgcgtgcc gcagttgcac gcgagaagag tggccataag
     4201 tggcaagaat gatcggccac ccaaaaccga cacgagggcc aggttcaccc cacagcaagc
     4261 agaagactcc tttatcgcgt cccaatccac aggaatcgcc cgatgcggca atcaaaggta
     4321 ctctgcctgt tcccatcccg ggaaaattcc ggagcaccag ccttagcagc cccagaaacc
     4381 agccgacggc caggttcacc ccacagcaag cagaagaatc ctttatcggg gcccaatcca
     4441 caggaatcgc ctgatgcggc agccaaaggt actctgcttg ttccatcctg ggaagccggc
     4501 cccacaacat ctgccccagc cttgtccgga ttcctgcttt tcggcctcga gccgcccggc
     4561 agaggccgag gattcatctc gtggcccctt gcaccccccg ttgaaagcgg cttcagccac
     4621 ccaacgccga tccaggcccg ctggcggcgt accaacgtga tgatctgggc ttgaagcgcg
     4681 tcaagtccct ggcgtcaacc agaaagggcg ctcgaaaacc ggcgtgacac aaatgacttc
     4741 caggcgggct gctgaccgta ccttaaccaa ccgcctcttc gctggctgcc gggtcttgct
     4801 gaacgctgaa cgccgaacgc tgaacgctcg tccttttccc ctggatctcg atgtacacat
     4861 tcaccagact cagcgccgcc ggcgtcaccc ccggaatccg gctcgcctgg cccagcgtcg
     4921 ccggccgcac ccgcatcagc ttctcctgca tctcgcgcga cagcccgctc acctgcgtgt
     4981 aatcgaacca ctccggaatc ccgcgctcct ccgccctctt cagcttctcg atcgctttct
     5041 tctgctggtc gagatagccc gcgtacttga tctccgtctc cacggtcttc agctcgttgt
     5101 gcgcccgcgc gcccaaacgt ccctccgccc ccgcctccat ccatggcgcg aagaccggat
     5161 cctcgagctg ctcacgcaaa gccggccaga ccgtctccat cgtcacttcc ggccgccgca
     5221 gaagctgacc ccagctttgg cccgctggcc aggaacccga tccgccatcg gcgtctccat
     5281 gcgcgtcgct gtcctgtgcg cgaagcgcgc ttccgcctgg acggcaagtc caaagcacct
     5341 tctccagcgc caccgcgcgc gcctgcttcg cctcgtactc cgcccaggcg tcatccccga
     5401 tcagccccag ccgccgtcca tgcggcgtca gccgccggtc tgcattgtcg atgcgcaaat
     5461 gcagccggaa ctccgcccgc gacgtgaaca tccgatacgg ctcgttcgtc cccttcgaaa
     5521 tcaaatcgtc gatcaaaatt cccgtgtacg cctcggtcct gtccagcgtg aacgccgcct
     5581 cgcctttcac gctgagcgcc gcgttgatcc ccgccatcag accctggcac gccgcctctt
     5641 cgtaccccga cgttccgttg atttgccccg ccagatacag ccccgcaaac ttcttcaccc
     5701 gcagcccacg gtccagctcc gtcggatcga tcgcgtcgta ctcgatcgcg taccccggcc
     5761 gcagcatctc cgcgttctca agccccggaa tcgaccgcac aatctgccac tgcacctcca
     5821 tcggcagcga cgtcgacatc ccgttcacat acacctcgtg cgtgttcagc ccctccggct
     5881 ccaggaaaaa ctggtgctgt tccttgtccg gaaacttcac caccttgtcc tcaatcgacg
     5941 gacagtatcg cggcccgatc cccgtgattt gccccgaata cataggcgaa cggtgcacgt
     6001 tttcgcgaat gatccgcagc gtctccggtg tcgtgtacgc gatgtggcac gaaacctgcc
     6061 gctgcggcag cgtcttcgtc cggaaactaa acggcgtcgg atccgcatcg cccggctgct
     6121 cggcaaattt cgaccagtca atcgttcgcc catcgagccg tggcggcgtg cccgtcttca
     6181 gccggcatcc gcgcagcccc agcttcttca gcgcctcacc cagcagcacc gatggcggct
     6241 cgccgctccg ccccgccgga tagttctcct cgccgcaatg gatcaaccca ttcaaaaatg
     6301 tgcccgtcgt gatgacagtc gctccggcat aaaccactcg cccatcccgc agcttcaccc
     6361 caaccacccg cggcaactcc ccgccgtcgc tgtcttccgg aacgccgaac gccgaacacc
     6421 gatcgtcgtc ctgaacgctg atcgccgaac gctgaacgct ggtcatcgtg tccagcacaa
     6481 cgtccaccac ctccgcctgc ttgatgaaca atcccgcctg tgactccagc acctcgcgca
     6541 tcttcacgcg atactgctgc ttgtcgcact gcgcgcgcgg cgaccatact gccggccccc
     6601 gcgacgtgtt cagcagccgg aactggatcc ccaccgcatc ggccacctcg cccatgatcc
     6661 cgcccagcgc gtccacctcg cgcaccaggt gccccttcgc gatcccgccg atcgccggat
     6721 tgcagctcat ctgcgcaatc aaatccagat tcagcgtgaa cagcgcggtc cgcagcccca
     6781 tgcgcgccgc ggccatcgcc gcctcgcacc cggcatggcc cgcgccaacc acaacaacgt
     6841 cgtattgctc tgtaaaaacc atggggacgg ggacgtaccc cttcattcta ccggcccgcc
     6901 gcgaccgcat gccgccaccg gatgggccgc attggccgtg acacaaatat ccgatatcta
     6961 aagcgtcgaa gtccgcctgg acgatccttg ccgcgaagcg gtgccgcgtc agcgacacac
     7021 ggtagaagcc ccggccttca agccggggga agggagcatc agatattcgg gcctttaggc
     7081 ccggagatgt actgaatcat gcaggcacgc tgccagtccg cgaagcgcgt tcgcatcgac
     7141 agccagtccc ccaaaagaaa gcggcagccc gaaggctgcc gcacgttgga tttgaggcgc
     7201 gaagcgcgac tgcctggacg gccagtcccg cgaagcgcaa ttgcctggac ggccagtcct
     7261 agaactggaa ccgcaacgcc atctgcagct gccgcacgtt gtacgcgaag ttgctgttat
     7321 ccgtgctggt cacgctctgg aagggcgtgc tcgtgtacgg tgtcagctgg ttcacgtggt
     7381 tcgtgttgtc gagcgccacc gaataagcag tggccccgat tccggtcacg ttctgatgat
     7441 tcgccaggtt gaacgcctca gccagaaact ccaggctgta gcgttccttc accacgaacc
     7501 gcttcgatgc ccgcagatcg acaccgaccg tatgcggcat gttgaacacg ttgcgatcga
     7561 caaacggcac acggttcgcc ccattcgacc cgttgaacga tccggtcgac accagcgatc
     7621 cctgtgccgt cgcacccggc gtcagataca gcttcgactg cgatccgctg atgcccatcg
     7681 aatacggcag ccccgattgg aactggaacg tgggcgcaag ctggaagtcg ttcgccagat
     7741 accccagcgg cccgtgcacg tgccacggcg aatccagcac gctgtacatc atcaggcggt
     7801 ttggtacgtt ctggttcgag ttcccgtagt ccagcttcag attcgctgga tccagcaacg
     7861 cattcgccgt tggtgccgtg ttgttgttct cgccgtagtc cagcgcatgc gaccaggtgt
     7921 agctcgcgct gaacgatgcg tagtgcgaca tccggtgctg gaactgagcc accagcgcct
     7981 cgtagttcga cgtgaccccg ctgaacgtat ccgtgatcga gctgaaattc gtgttcggcc
     8041 gctttcccag ataggcgttc gtcgtcagcg tcgtgccgtc cgccagcgga cccttcccgc
     8101 ccggatcgct gatcgtaaag ctcacctgcg tcggagccgg caggttggtg tcgacaaagt
     8161 ccggcagccg ccgcccccac gatcccagcc acgacaggcc aaatacatcg ttcttcgtga
     8221 tctgctgctg caacgacagg tcgccctggt aaatcgccgg caggcggaag ttcttgtcga
     8281 agaaatacac cgtcgcgttc ccgcttgccc ccagcagaca gctgccgtag ctgctcggcg
     8341 gcaccacctg cgggaactgc ggcgcgcacg ccgcgcccgg attgaactgc gggttcgtct
     8401 ggcttcccga agctccggtc acgatcaggt tctgatagat ggtcgcgtta atcgcccgcg
     8461 caaaaaagaa tccgtatccg ccgcgtacca ccgtcgtccc atggccgaat acgtcatacg
     8521 caaatcccac acgcggcccg atgttgccct tgttgctcgg catctttgat gttgctggca
     8581 ccagcgaatt ggccagcatc ggatccggca tcttctcgaa ctcccaccgc aacccgcccg
     8641 tcaggctcag tcgcggccgg atcttccact catcctgcac gaagagcgaa tagtcgcccg
     8701 atgtgaactc caggcccgga agtcccgcac cctgattgaa gtacgtgtag tgctccgccg
     8761 tcttgcccgg ctgcggattc tgcgaaagat acaaatcggc cagatagttc cccagcggag
     8821 tgttgccgct gtaggtaaag ccaccatact ggttgtacaa attggaaatt agatcgtacg
     8881 tgtggatgta gtctccgccg aaccgcacgt cgtggttccc gtggatccat tccagagtgt
     8941 ccgcaatctg ccagcgccgc tcatccggca gcgccgcgcg gttcaggaac tgcggcgtgc
     9001 cgaactggaa gaagccgctc agatacacgt tcgcaggaag tcccagggaa ttcgtgtaat
     9061 tcgccgtctt caccaggttg ttctgctcgt acgacgtcgg cgtctgattg aactcgaact
     9121 caaaatcgcg cccgtacatg taacgcactt cgttgctgaa agtcggcgtc aggaacgtgt
     9181 ccagcttgcc cacgatccag ttatcgcgaa cgtagtcgtt gccgaagctc gacatgccgt
     9241 acgccaccgc agggctggtc tggatgcccg ccggcgacgt ccaccgcatc cggttcgctt
     9301 ccaccgacgc ccgattctta tcgttgatct gccagtcaat cttggggaaa taaatcgtct
     9361 gatcgcccgt gcgcgccact tgtcccagca tcgacgtcat gctcgcgatg ccattgctgt
     9421 agtagctggc cgcgtcagca tagctcagtc ccgtgttggt ggagaactgg cagacattcg
     9481 aatcctgata gttcggagcc gtcgttccac tggtcactcc gcacactttt cctgccggca
     9541 gagtcgcgtc cggtgccgta tagaagttcg cattcgcgct cgttccggtg atcgtcgccg
     9601 cgcccggaaa atcatgatag aagcgatccg ctccaaggaa gaagaaaagc ctgttcttca
     9661 aaaggggccc gcccacggtg aatccatact gccgccgcaa atcggtaggc ttgaacggaa
     9721 ccgccgtgta atgccccggc gacgtctgca ccggatgttg tacgtaatag ttctgcgcat
     9781 tccagtcgct gtcccgatcg aagtaataca catcgccgtg gaacgcgttc gtcccgctct
     9841 tcgtcaccga gttgacgatg cctcccgccg accgcccgta ctccgacgag tagttcgacg
     9901 tgttcacctg gaattcctgc accgagtcct taatcgtcga gtagcccgcc cgcgtgcgcc
     9961 cgcgctcctc ggagaagtac gcctggttat cgtccgtccc gtcgatcgtc acgttattca
    10021 gcagcgtgct cgcgccgcga aaactcagca acccgtatcc gttcgagtcg ttcgtcactc
    10081 caggcgtcag cagcgcaaac gccgaccacc ggtaattgtt ctccggcaga tccttcagcg
    10141 gcacctgatc cacaaccccg gcgaagtccg ccgacgttgt gttgatgatc ggcgcctccc
    10201 ccgtcacctc aaccgtctgc gcggccgtgc ctacttccat ccttccctca acgtccgtca
    10261 tcgatcccac ctgcaccgtc acatccgtcg ccttgtaatc gcggaagccc ggggcgctga
    10321 tgctcacgct gtaaaccccc ggttgcaggt ggatgatccg gtaatatccg ttggaatcgg
    10381 tcgtcgccgt ttgctgcgcg ttcgtcgcca cattccgcac cgtgaccgcc gagccggata
    10441 ccaccgctcc gctggaatcc atcaccgtgc ccccgatcgc accgtcgacc gccgtttgtc
    10501 cgtgcaaaac cggtgttgcg aagaacgcca gaaccaccaa agccaaaaat aacgtacaga
    10561 gtcggttaca aatttcgcgc ataaagcctc tccaaattca caacgttgta ggttgacgcg
    10621 cggcttacag gcttgttctg gccttcccgc gcttgttttt gacgaaattt cttcggctga
    10681 gatcgatggg ccctctcttg ccgcttcctt cgtggcaaaa cggtccgctt tcctctcgat
    10741 aactaacctg agcgtcacat atttagttga acgtgtgatt acagcatgga aagggaagca
    10801 atgttgccac aatcggcgct cgctgttgtg gaaaacgctc cgaaatccac cgtcactgtg
    10861 acaaaataac gctctctccg aacggcttcg gtggaaatct cgccacgatt ctttttccga
    10921 acaccggcgc cgtcatcaga atcgcagccg cagccccacc tgcagttgcc gctcgcggct
    10981 caccgcgccg ttgttggcgt tcgtcacccc accaaaagcc gccgtcggat cataaacata
    11041 ctgcgtttcc gttcgcccgt tcaccagcac cgtcttcgtc cccggcttca tcccgctctg
    11101 ccacgtcagc gtcgccatat tcgcgtgcga cttgtcgttc gtctctcggt aaccgatgct
    11161 ctgcatgttc gtaacgttcc gatggttcag cgcgttgaac gcctccccca tcacctcgaa
    11221 tgagcaacgg tcgctgatac gaatcgtctt cgtgaatcgc aaatccagac tcactgccgc
    11281 tggatagcga aacgtgtttc gcccaatcgg cggaatcaga tcctcgcctc ccgacccgtt
    11341 caggctgccc cccaggctcg caatcggcac cgccatccct gccgtgttgt ccgtgaatgt
    11401 ctcattgggc tgggtcaccg ccttcaggca attcgcgccg ccgcccgtcg ccccgcccgc
    11461 attcagccag ttcgcatatg agcacgacgg cgtcggcacc gcccctgaag tccgcatcga
    11521 ccacggaagc cccgttcgcc acgcgcctgt cgcacccagc gaatatccgc ccagcagcat
    11581 tcctgctggt ccccgcagcc gccacggcgc gcgcagcact acgccgcccg ccacccgctg
    11641 ccgcacatcg tagtccgacg tcccatgctc catctgcaaa tccgccggat cgtaaacgtc
    11701 attccgttcc gcaaacgtcg ccgcattctg gttatcgtcg atcgtgtgcg accacgtata
    11761 ggccgcattc gccgtcaccg tccgcgacat ccgccgcgtc aactgcacca ccgctccgcg
    11821 atagctcgag ttggtctcgc tcaccatcgc agtgatcgcg ccataggccg gattcagccg
    11881 ctgataataa aacttccgcg gctcgtaaac ctgattcgtg taccccgata caccgctcgt
    11941 cgccttcccc agcggcccaa tcgccccctc gtttcccggc gcccgcacgc tgtagaacac
    12001 cgtcgcccag ttttcgagat cgatattttt gtccacgaat tgtgtcagga accgcccatc
    12061 tgtcgccagc cccgtcagcg tcagcaccgt cctgaatccc agctcctgct gcagcgaaac
    12121 ctccatcgaa taaatctgcg gacgccgaaa atgcggatcg aaataaaccg cctccggcgc
    12181 agtcgaattg tggttcggcg ccgtcggatt cacatacggc ttcgcgtcac tcacaaatac
    12241 atagggaaac gccggcgcgg ccacatccag cggccgatac gaatacgtcc gcggcgatcg
    12301 cgccgacccc gtcgtcgtca gcacgctgaa caccatcgca ttcggcaccc gcgccgtgta
    12361 gatcccgtaa ccgccccgca ataccgtccg ccctttgccg aaaacatcca gcgcgaaccc
    12421 gccgcgtggc ccgaaacccc tccgatcgtg cgccaggcgc gcggtttccg gaatgtccgc
    12481 gttcaccagc gccgcatttg tatcaggcac c
//
LOCUS       DASZYN010000221         2524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1229401, whole genome shotgun sequence.
ACCESSION   DASZYN010000221 DASZYN010000000
VERSION     DASZYN010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229401"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="VHE33_05740"
     CDS             complement(<1..1040)
                     /locus_tag="VHE33_05740"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HVT96990.1"
                     /translation="MPDPIRVLIVDDSAMMRQLLSQILTSDAQLSVIGVAADPYVARD
                     KIKALNPDVLTLDIEMPRMDGLTFLEKLMRGHPMPVVMISSLTEKGADTTLRALSLGA
                     VDYVSKPKLDVSSGTIEQAEEIIEKVKAAARVKIRTGAIPAAAAGVETVRRPMQFSAT
                     HKVVAIGASTGGTEALKELLTPLPADFPGIVMVQHMPEAFTGPFAKRLDGMCRIHVQE
                     ARDGDRILPGHALLAPGGHQMAVVRRGAEYGVRVYRGERVNRHLPSVDVLFNSCAAQM
                     GRNALGVLLTGMGNDGAQGMLRMKEAESYTIAQDEASSVVFGMPKEAIRLGAVDQVLP
                     LHRIAGELMDHLG"
     gene            complement(1058..1690)
                     /locus_tag="VHE33_05745"
     CDS             complement(1058..1690)
                     /locus_tag="VHE33_05745"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HVT96991.1"
                     /translation="MSPALSVSQIRRHGLRAVPHPARPGFLRAHHEPQRRVDIGDVVA
                     SRHPLCLHTVLGSCVAVCLWDPVAAAGGMNHILVPSSHADCQCGSRCGVHAMELLINA
                     VMKAGGDRRRFVAKAFGAANVLPVFQSPTVGDLNARFVREFLATERIPLLAERMGGNR
                     AVRAVFHAHSGRAFVHTVDGSRLPEIVREETAWFNRPLTERFADEEPVLF"
     gene            complement(1687..>2524)
                     /locus_tag="VHE33_05750"
     CDS             complement(1687..>2524)
                     /locus_tag="VHE33_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase"
                     /protein_id="HVT96992.1"
                     /translation="TGIWLRDGKQVMLASRLSRRLRAHGLTNFADYYNYLDGLPDQGT
                     ELGELINCVTTNKTAFFREPHHFRFLGETVVPETMARMRGEGNGTGQSGSQYGSQYGI
                     RIWSAACSTGEEPYSIAITLLEAQSRWHWPGTASAMRIVASDIDTSVLEKAARGIYAA
                     DEVEEMEPSLLKKHFLRGTDEMEGQVRVKKPVAALVEFERINLMDRSWPISGPFDAIF
                     FRNALIYFRQETQDLFLRRMIRYLRPGGYLFLGHSEHIPWMQDVLEPLQQTIYRVRSA
                     AR"
BASE COUNT          464 a          952 c          702 g          406 t
ORIGIN      
        1 ccgaggtgat ccatcaactc ccccgcgatc cgatgcagcg gcagcacctg atcgaccgcc
       61 cccagccgga tcgcctcctt cggcatccca aagaccaccg agctggcctc gtcctgcgcg
      121 atcgtgtagc tctccgcctc cttcatccgc agcatcccct gcgccccatc gttccccatc
      181 cccgtcagca gcacgcccag cgcattccgc cccatctgcg ccgcgcacga gttgaacagc
      241 acatccaccg acggaagatg ccggttcacc cgctcgcccc gatacacccg caccccatat
      301 tcggccccgc gccgcaccac cgccatctga tgccccccgg gagccagcag cgcatgacct
      361 ggcaggatgc gatcgccatc ccgcgcctcc tgcacgtgaa tgcggcacat cccatccagc
      421 cgcttcgcga acggcccggt aaaggcctcc ggcatatgct gcaccatcac gatgcccgga
      481 aaatccgccg gcagcggcgt cagcaactcc ttcagcgcct cggtgcctcc cgtcgacgcc
      541 ccaatcgcca ccaccttgtg cgtcgccgag aactgcatcg gccgccgcac cgtctccacc
      601 ccagccgcag ccgccggtat tgcccccgtc cggatcttga cccgcgccgc ggctttgacc
      661 ttctcgatga tctcctcggc ctgctcgatc gtcccgctcg agacatccag cttcggcttc
      721 gaaacgtaat cgaccgcacc cagactcagc gcacgcagcg tcgtgtccgc gcccttttcg
      781 gtcagcgacg agatcatcac caccggcatc ggatgccctc gcatcagctt ctcgaggaac
      841 gtcagcccgt ccatgcgcgg catctcgatg tcgagcgtca gcacatcggg gttgagcgcc
      901 ttgatcttgt cccgcgccac atacggatcg gcggcgacac cgatcacgct cagctgcgca
      961 tcggaggtga ggatctggct gagcagctgg cgcatcatcg ccgagtcgtc gacaatgagc
     1021 acgcgtatcg gatcgggcat cgcagacctc caggccgtca gaaaaggaca ggctcctcgt
     1081 cggcaaaacg ttccgtgagc ggccgattga accaggccgt ctcctcgcgc acgatctccg
     1141 gcaggcgcga gccatccacc gtatgaacaa acgcgcgccc cgagtgagcg tgaaagaccg
     1201 cccgcaccgc ccgattgccg cccatccgct cggccagcag aggaatcctc tccgtcgcca
     1261 gaaactcgcg cacgaatcgc gcgttcaggt cgcccaccgt cgggctctga aacacgggca
     1321 gcacattggc cgcgccaaac gccttcgcga caaagcgccg ccggtccccg cccgccttca
     1381 tcaccgcgtt gatgagcagc tccatcgcat gcacgccaca gcgggagccg cactgacagt
     1441 ccgcatggga gctgggcacc aggatgtgat tcatgccgcc ggctgcggcc accggatccc
     1501 acagacagac agcaacacac gagccgagca ccgtgtgcag gcacagcgga tggcggctcg
     1561 ccaccacatc gccgatgtcc acccgccgct gcggttcatg atgcgcgcgc agaaagcccg
     1621 gccgcgccgg atgcggtact gcccgcagcc cgtggcggcg gatttgcgat acggagaggg
     1681 ccgggctcat cgcgctgcgc tcctcacgcg ataaatggtc tgctgcagcg gctccagcac
     1741 gtcctgcatc cacggaatgt gctccgagtg ccccagaaag agatacccgc ccgggcgcag
     1801 gtagcggatc atccggcgca aaaacagatc ctgcgtctcc tgcctgaaat agatcagcgc
     1861 attgcggaag aagatcgcat cgaaaggccc gctgatgggc cacgagcgat ccatcagatt
     1921 gatccgctcg aactccacca gagccgcgac cggtttcttc acgcgcacct gaccctccat
     1981 ctcgtcggtg ccgcgcagga aatgcttctt cagcagcgag ggttccatct cctccacctc
     2041 gtcggcggca tagatgccac gcgccgcctt ctccagaacc gacgtgtcga tatcggacgc
     2101 gacaatgcgc atcgccgacg cggtccccgg ccagtgccag cgactctgcg cctccagcaa
     2161 cgtgatggcg atcgaataag gctcttctcc cgtcgaacag gccgcgctcc agatgcgaat
     2221 tccgtactgg cttccgtact ggcttccgga ctggcccgtg ccgttgcctt ccccgcgcat
     2281 gcgagccatc gtctccggca ccaccgtctc gccgagaaac cggaagtggt gcggctcgcg
     2341 aaagaacgcg gtcttgttcg tcgtcacgca gttgatcagc tcgccgagct cggtcccctg
     2401 gtccggcagc ccgtcaaggt agttgtagta atcggcaaag ttggtcaggc catgcgcgcg
     2461 cagacgccgc gacagccgcg atgccagcat cacctgcttg ccgtcgcgca gccagatgcc
     2521 ggta
//
LOCUS       DASZYN010000222         1571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_776678, whole genome shotgun sequence.
ACCESSION   DASZYN010000222 DASZYN010000000
VERSION     DASZYN010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776678"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1112)
                     /locus_tag="VHE33_05755"
     CDS             complement(117..1112)
                     /locus_tag="VHE33_05755"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HVT96993.1"
                     /translation="MFLIAGARQFFTGVWDKYDLDLQDFLHDKLERLVGVALLCWLLV
                     WLLGVISRHILRLAERQSGTTGHYAQVRTLTSVIRATGLGVIIVLATLQVLSVFGFNL
                     GPLLTSAGVAGVAIGLAAQNIVKDCFNGFLILIEDQYNVGDVVTVAGVSGTVETMTLR
                     RTQVRGADGTLYIIPNSQITVVANLLRGYSLATISVSVDFSANPDAVMQVLRDAAMSV
                     RNDPQYRDVYLADPILLGVDAIKGSQVIYPVQLKTKANQQWAAQRETQRRIRLALEEH
                     GMLPGDPMRVYARDGDPRSAIGVQSAGAAKGHEHVAEKTDPTAAKPTEGNPLTGE"
     gene            1234..>1571
                     /locus_tag="VHE33_05760"
     CDS             1234..>1571
                     /locus_tag="VHE33_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96994.1"
                     /translation="MTRMALSVRSTWLPAISLAFILAAPAAGAQQSTPATPRRQSSAT
                     TDTAVYPDMPGSGTRDSEQSRMLREMSRDRNKLRQKEIVEDTNHLMDLVRQLKDAVDK
                     SNKDQLSLSVV"
BASE COUNT          297 a          572 c          417 g          285 t
ORIGIN      
        1 aacgatggca cccgcccaac tgcggacggg ccgcaggatg ttccaaacgc cgagcgcgga
       61 acgtcgaacg ccaggttttc tgaacgctga ccactgacaa ccgaggtctg ttcttctcac
      121 tcccccgtca gcggattccc ctccgtcggc ttcgctgccg tcggatccgt cttttctgcc
      181 acatgctcgt gtccctttgc ggccccagcg ctctgaacgc cgatcgccga acgcggatca
      241 ccgtctcgcg cgtacacccg catcgggtct cccggcagca tcccgtgctc ctccagcgcc
      301 aggcggatgc gccgctgcgt ctcccgctgc gccgcccact gctggtttgc cttcgttttc
      361 agctgcaccg gatagatcac ctgggatcct ttaatggcgt ctacccccag caggatcggg
      421 tcggccaggt acacgtccct gtactgcgga tcgttgcgca cgctcatcgc tgcatctctc
      481 agcacctgca tcaccgcatc cggattcgcc gaaaaatcca ccgacacgct gatcgtcgcc
      541 agcgaatagc cgcgcagcag attcgccacc accgtgatct gcgagttggg aatgatgtac
      601 agcgtgccat ctgcaccccg cacctgggtg cggcgcaggg tcatcgtttc caccgtgccg
      661 ctcacgcccg caaccgtgac cacatcgccc acgttgtact ggtcctcgat caggatcaga
      721 aatccgttga agcaatcctt gacgatgttc tgcgccgcca gcccgatggc cacgcccgcc
      781 acaccagcgc tggtcagcag aggccccaga ttgaaaccga aaaccgacag gacctgcagg
      841 gtggccagga cgatgatcac gccgagtccg gtggcacgaa tcactgacgt cagcgtccgc
      901 acctgggcgt agtgtccggt cgtgccggac tggcgttccg ccagcctgag gatgtgccgg
      961 ctgattaccc ccagcagcca aaccaggagc cagcacagca gcgcgacgcc gaccagcctc
     1021 tccagcttgt cgtgcaggaa atcctgcaga tcgagatcgt acttgtccca caccccggta
     1081 aagaactgcc gagctccggc tatcagaaac aaggtccgca tcgccttcac agcataccgt
     1141 tcccggccct atcctccggc tgcgaattcc ggcttataat tcttcccgac cggcggcgcg
     1201 ctcacgcccg ccccaccgcc cggaggagac atcatgaccc gcatggccct ttctgtccgg
     1261 agcacctggc tccctgcgat ctccctcgct ttcatccttg ccgcacccgc tgcaggcgca
     1321 cagcaatcca cgccggcgac accccggcgc cagtccagcg ccaccaccga taccgctgtt
     1381 tatcccgata tgccgggctc cggcactcgc gactccgagc agtcccgtat gctccgcgag
     1441 atgtcgcgtg accgtaacaa gctacgtcaa aaggaaatcg tcgaggatac gaatcatctg
     1501 atggacctgg tgagacagct caaggacgcg gtcgacaaga gcaacaagga ccagctgtcc
     1561 ctttcagtcg t
//
LOCUS       DASZYN010000223         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_648927, whole genome shotgun sequence.
ACCESSION   DASZYN010000223 DASZYN010000000
VERSION     DASZYN010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648927"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VHE33_05765"
     CDS             complement(<1..571)
                     /locus_tag="VHE33_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT96995.1"
                     /translation="MPTTTASPSPLVSAPRAPRFTVDAVRRACAGTFDLVVAAYACAI
                     VLWAVRGFVDLGRLTPDSAEKPLLILLVVVPIRVAIGGRSWLVDWCASPWVRRRVDIL
                     FRAPIPAAVTDTMFALLATRLATTAVSFVADVLFPPSRLRGFVMPFRQRHFAEIFAAW
                     DSGWYFDIAHHGYFWRPDGQSSVAFFPLYP"
     gene            complement(671..787)
                     /gene="rrf"
                     /locus_tag="VHE33_05770"
     rRNA            complement(671..787)
                     /gene="rrf"
                     /locus_tag="VHE33_05770"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(959..>2491)
                     /locus_tag="VHE33_05775"
     rRNA            complement(959..>2491)
                     /locus_tag="VHE33_05775"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          527 a          772 c          716 g          476 t
ORIGIN      
        1 tcgggtagag cgggaagaac gcgacgctgc tctgcccgtc ggggcgccag aagtatccgt
       61 ggtgggcgat atcgaagtac cagccggagt cccacgccgc gaagatctcg gcaaagtgcc
      121 gctgccggaa cggcatgacg aagccgcgca ggcgcgacgg cgggaagagc acgtccgcga
      181 cgaacgagac ggccgtcgtc gcgaggcgcg tcgcgagcag cgcgaacatc gtgtcggtga
      241 ccgcggcggg aataggcgcc ctgaacagga tatcgacgcg gcggcgcacc cagggcgacg
      301 cgcaccagtc gacgagccac gagcggccgc cgatcgcgac gcggatcggg acgacgacga
      361 gcaggatgag gagcggcttt tccgccgagt cgggcgtcag gcggccgaga tcgacgaacc
      421 cccggacggc ccacaggacg atcgcgcacg cgtaggcggc gaccacgagg tcgaaggtgc
      481 ccgcgcaggc gcggcgcacg gcgtcgacgg tgaatctggg cgcgcgcggc gcggacacga
      541 gcggcgacgg gctggcggtc gtcgtcggca cggggggagt atgttacgga cgagctctgg
      601 cgagcgcaag aggaatttgg gagtacaaaa agagagcccg ctgtcttgcg acagcgggct
      661 cgacgaatac tcccggcagc gacctactct cccacgcagt ttcccacgca gtaccatcgg
      721 cggtagcagg cttaacttcc gtgttcggaa tgggaacggg tgtgaccctg ctcctatgac
      781 caccgggaaa ggcgatgtcc gccgtcgcct gcggcgcagg cggacaaact tattgtgcct
      841 gggatcctgc gacaatcgcg tctgatatcg gcgcgggatc gaaggtcgtc ccagacaatg
      901 tcaaagagcg actgaatatc ctgcaaacgg tagtttctcc cgccacggag agaaaataat
      961 ggtcaagcct cacggctgat tagtactggt tagctccacg tctcgcgacg cttccacacc
     1021 cagcctatta cctggtagtc ttccaggagc ctttagaggc cttgcggcct gggacatctc
     1081 atcttgggga gtgtttcacg cttagatgcc ttcagcgttt ctcacgtccg aacgtagcta
     1141 cccggcgcta cccttggcag gataaccggt acactagagg ttcgttcaac ccggtcctct
     1201 cgtactaagg tcaagccccc gcaaatgtcc tccgcccatg atagatagag accgaactgt
     1261 ctcgcgacgt tctaaaccca gctcacgtac cgctttaacc ggcgaacagc cggacccttg
     1321 gaacctgcta cagctccagg atgcgatgag ccgacatcga ggtgccaaac ctgagcgtcg
     1381 atgtgaactc ttggctcaga tcagcctgtt atccccggcg taccttttat ccgttgagcg
     1441 atggcgcttc cacacgcaac caccggatca ctatgcccag ctttcgctcc tgcttgacgt
     1501 gtcagtctcg cagtcaagct ctcttgtgcc attgcactcg acggctgatt gccaaccagc
     1561 ctgagagaac ctttgggcgc ctccgttact ttttaggagg cgaccgcccc agtcaaacta
     1621 cccatctggc gctgtccccc acccggataa cgggcgaagg ttagagtccc ggcataaaca
     1681 gggtggtatt tcaacgttga ctccaccatg gctggcgcca cagcttccta gtctcccacc
     1741 tatcctacac aattcatacc aaaacccaac accaaactgt agtaaaggtg cacggggtct
     1801 ttccgtcctg atgcagctaa cgagcatctt tactcgtact tcaatttcgc cgggtctgtg
     1861 gttgagacag taggaaagtc gttacgccat tcgtgcaggt cggaacttca ccatatcccc
     1921 ttctttcgaa ggggggcaga ctatgccttc atctccatga gactccgaac aatcgaatcc
     1981 ggatggtatc cgcgctttcc gctcggattc atctgggctg ccaaccgtac aatcaactgc
     2041 aatccggtcc gttcaagatg ctctccatcc accatcctct ggcagatgtc agcgaacagc
     2101 tcaaagtcct tttgcttccc ggacaacagt gggaaagcct tgaagtgcgg aatgaccttg
     2161 gtaaccaaga tcggaaggct acgcacctcc cacttcaacg tcttgtcact tctgtcaggc
     2221 cggagggtgc cgcatccgaa gtgctgctgc atcagctgca gtacttcaga gcggtcacca
     2281 ttctgactga ccgacagact cggtctcacc tcccatttga cgccaagcgt cttcctggga
     2341 ctgatcgaga ccgtgaagca tccctcaccg tcgacaaacc ctgagaggta cgacggaaga
     2401 tcgattgctc ctcgtagaga gcccgcgtat tgccccggat gtgccacgtc actgaccgta
     2461 ggccggcgtt gtgacaggga cccgaagcct c
//
LOCUS       DASZYN010000224         3932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_908571, whole genome shotgun sequence.
ACCESSION   DASZYN010000224 DASZYN010000000
VERSION     DASZYN010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908571"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VHE33_05780"
     CDS             <1..283
                     /locus_tag="VHE33_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HVT96996.1"
                     /translation="GNRDVFALQVRGESMRDEHILDGDFVLVERTATARSGEIVVALV
                     RGSETTLKRYYREGSVIRLQPANIEMDPILVPAAQVAIQGRVLGVLRKY"
     gene            complement(350..1549)
                     /locus_tag="VHE33_05785"
     CDS             complement(350..1549)
                     /locus_tag="VHE33_05785"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVT96997.1"
                     /translation="MRRAVRIGVLLVLVVCCGLAQAQSGFAAIDSLVDHAVEEHLLPG
                     AVVVVGHDGRVVFRKAYGMRSLEPVRETMTVDTIFDMASLTKCLATATAVMQLYQKGK
                     LQFDDPVAKYLPAFGANGKQDITIRELLTHYSGLPPDLPLAEPWEGHGTAYQMAFAIA
                     PAGPPGEKFVYSDINFIVLGALVEKLSGMTLNEYAERFIFQPLGMRHTRFLPPESWIP
                     HIAPTQYEHGASAYGVAGLATGPGDVMLRGVVHDPTSRRMGGVAGHAGVFSTADDVAV
                     YAQNLLNRLAGLPSRFPLSRRVLALMVKPEQPAGGKAVRGFGWDIDSPYSSNRGTIFP
                     VGSFGHTGFTGTSLWMDLASDTYVIVLANSVHPNGPKRIVALRGKIADAAARAVGAEK
                     HAAASSR"
     gene            complement(1546..3075)
                     /locus_tag="VHE33_05790"
     CDS             complement(1546..3075)
                     /locus_tag="VHE33_05790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HVT96998.1"
                     /translation="MTPPLAHSIHLRTVDLVLVGVYLIGITLFGLRFRKSRQTSLRSY
                     FLADRNLPWWAIALSIVAAETSTLTIISVPGLAFTGDFGFLQIALGYLLGRGVICILF
                     LPRYFRGELLTAYQLIGQRFGPRLHRFTAGLFLVMRAAAEGVRVFAVAIVVGVAIGTG
                     DVASIAILSVLTLIYTFEGGMTAVIWTDVVQMFLYVAGSLVALATVCHHIPGGWSGLI
                     AGASAAHKLTVLHFSWSLAETYTFQAGVIGGCFLTMASHGTDQLMVQRLLAAKNLRES
                     RVALLASGGVIFFQFTLFLLIGAGLWVYYGHGGHAPGLSADRLFPAFIVEQMPEGIAG
                     LMIAAILAAAMSNLSAALNSLASTSVVDFYLPRYPDISDTMRTRLSRLMTLVWAGVLF
                     ALAMFSRGGGHVVEIGLSIASVLWGAMLGVFLLGTLTRRADERGTIWGLAAGVVVNLL
                     LWLQPSALALPVNGHVLVIPKIAWTWWVLIGSLVTCVVGYGVSVIFPEGEKRSEEGVE
                     G"
     gene            complement(3084..>3932)
                     /locus_tag="VHE33_05795"
     CDS             complement(3084..>3932)
                     /locus_tag="VHE33_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HVT96999.1"
                     /translation="VARVQTGTIKRMKADAETAEANLRRGLEELRARTGVERGNVARC
                     CVGTAGETVPLVVDWLRDAFARHVGGELIIEGDVDVALDSVFFGQRGVLVLAGTGSNV
                     AGRAATGEIVTAGGWGPALADQGSGHFLGLEGLRRGFLAIDERRTTRLPDIAMEFWKL
                     GSLGELIQFANANPAPEYSKLAPLVVEAAEAGDAVAKEVVEQGGSDLAMLAGLVIEHL
                     RDLEASSDKEFVVPAVAVAGSILECAAPVREALIAGLRARYPEITVMEKPADPPAGAL
                     WRARQG"
BASE COUNT          834 a         1538 c          961 g          599 t
ORIGIN      
        1 cggcaaccgc gatgtcttcg ctctccaggt ccgcggcgaa tccatgcgcg atgagcacat
       61 cctcgacggc gacttcgtcc tcgtcgaacg cacggcgacc gcccgctccg gcgaaatcgt
      121 ggtcgctctg gtccgcggct ctgaaacaac gctgaagcgc tattaccgcg aaggctctgt
      181 gatccggctg cagcctgcca acatcgaaat ggaccctatc ctcgtgcccg ccgcacaggt
      241 cgcgatccag ggcagagtcc ttggcgtgct ccgtaaatat taggaatccg cggtttcctt
      301 caggccgcaa acgacaagcc caccaaccca ccccaggtcc acgcgcccct cagcgagaac
      361 tggccgccgc gtgcttctcc gctcccaccg ccctcgccgc cgcatcggca atcttgccgc
      421 gcagggcgac gatccgcttc ggcccattcg gatggaccga attcgccagc acaatcacat
      481 acgtgtcgct cgctagatcc atccacagcg acgtccccgt gaagcccgtg tgcccgaacg
      541 accccaccgg aaaaatcgtc ccgcgattgc tcgagtaggg cgaatcgatg tcccacccga
      601 atccacgtac cgccttgccc cccgccggct gctccggctt caccatcagt gcgagcaccc
      661 gtcgcgacag cggaaaccgg ctcggcagcc ccgccagccg attcagcaaa ttctgcgcat
      721 agaccgccac atcgtccgcc gtgctgaaca ccccggcatg tcccgccacg ccgcccatgc
      781 gccgcgacgt gggatcgtgc accaccccgc gcagcatcac gtcgccagga ccggtcgcca
      841 gccctgccac gccatacgcc gaggccccat gctcgtactg cgtcggcgca atgtgcggaa
      901 tccacgactc gggcggaaga aagcgcgtat gccgcattcc cagcggctga aagatgaatc
      961 gctccgcgta ctcattcagc gtcatccccg acagcttctc gaccagcgcg cccagcacga
     1021 taaagttgat gtcgctatag acaaacttct ctcccggcgg tcccgcaggc gcaatcgcaa
     1081 acgccatctg atacgccgtg ccgtgtccct cccagggctc ggccagcggc agatccggcg
     1141 gtagccccga atagtgcgtc aacagctccc gaatcgtaat gtcctgcttc ccgttggcgc
     1201 cgaacgccgg cagatacttc gccaccgggt cgtcaaactg caacttgccc ttctgataca
     1261 gctgcatcac cgccgtcgcc gtcgcgaggc acttcgtcaa cgacgccata tcgaagatcg
     1321 tgtccaccgt catggtctcc cgcaccggct ccagcgaccg catcccgtac gccttgcgaa
     1381 acaccacccg cccgtcgtgc cccacaacca ccaccgcgcc cggcagcaga tgttcttcca
     1441 ctgcgtgatc caccagcgaa tcgatggccg caaacccgct ctgcgcctgc gccagaccac
     1501 agcagacaac cagaaccagc aacactccga tgcggaccgc acgcctcatc cttcgactcc
     1561 ctcctcgctt cgcttctcgc cttccggaaa aatgacgctt accccatagc ccacaacgca
     1621 ggtcaccagc gacccgatca gcacccacca tgtccatgcg atcttcggaa tcaccagcac
     1681 gtgcccgttc accggcaacg ccagtgcact cggctgcagc cacagcagca gattcacaac
     1741 cacaccagcg gccagccccc agatggtgcc ccgctcatcg gcgcgccggg ttagcgtccc
     1801 cagcaaaaag actcccagca tcgcgcccca cagcaccgaa gcgatcgaga gcccgatctc
     1861 caccacgtgg ccgccgccgc gcgaaaacat cgccagcgcg aacagcaccc cggcccacac
     1921 cagcgtcatc agccgcgaaa gccgcgtacg catcgtgtcg gaaatatccg gatagcgcgg
     1981 caaatagaaa tccaccaccg aagtcgatgc cagcgaattc agcgccgcgc tcagattcga
     2041 catcgctgcc gccaggatcg ccgcaatcat cagccccgca atgccctccg gcatctgctc
     2101 cacaatgaag gccggaaaca gccggtctgc gctcagtccc ggtgcgtgtc ccccatgtcc
     2161 gtaataaacc caaagccctg cgccaatcag caaaaataac gtgaactgaa aaaagatcac
     2221 gcccccgctc gccagcagcg ctacccgcga ttcgcgcaga ttcttcgccg ccagcagccg
     2281 ctgcaccatc agctgatcgg tcccgtggct cgccatcgtg agaaagcatc ccccgataac
     2341 gccggcctgg aacgtatacg tctctgccag cgaccacgag aagtgcagca ccgtcagttt
     2401 gtgcgccgcc gaagcccccg caatcagccc gctccagcct cctggaatgt ggtgacagac
     2461 cgtcgccaaa gcaaccagcg atccagccac gtacaaaaac atctgcacca catcggtcca
     2521 gatcaccgcc gtcatgccgc cttcgaaggt gtagatcaac gtcagcaccg acaggatcgc
     2581 gatcgacgcg acatctccgg ttccaatcgc cactcccacc acgatcgcta cggcaaacac
     2641 ccgcacgcct tccgccgctg cccgcatcac caggaaaagt cccgccgtaa accgatgcag
     2701 ccgcggccca aagcgctggc cgatcagctg gtaagccgtc agcagttcac cgcggaaata
     2761 tcgcggcaga aacagaatgc agatcacccc gcgtcccaac agatagccca aagcaatctg
     2821 caaaaatcca aaatcgcccg tgaacgcgag accgggcacg ctgatgatcg tcagcgtgct
     2881 cgtctccgct gccacaatcg acagcgcaat cgcccaccac ggcaaattgc gatccgccag
     2941 gaaatacgag cgcagcgaag tttgccgcga cttccgaaat cgcaatccga acagcgtgat
     3001 cccaatcagg tacacgccca ccagcacaag atcgacagtc cgcaaatgaa tggaatgcgc
     3061 caggggagga gtcatcggtg cgatcagcct tgccgcgccc tccacaaagc gcctgccggc
     3121 ggatcggcgg gtttctccat caccgtaatc tccggatacc gagcgcgtag cccggcgatc
     3181 agcgcctcgc gtaccggcgc agcacactcc agaatgctcc cggcaacggc caccgccgga
     3241 accacaaact ccttgtcgct gctcgcctcg aggtcgcgca gatgctcaat caccagaccc
     3301 gccagcatcg ccagatccga gccgccctgc tcaaccactt ccttcgccac cgcatcgccc
     3361 gcctctgcag cctcaacgac cagcggtgcc agcttcgaat attccggtgc cggattggcg
     3421 ttcgcgaact ggatcaactc ccccagcgag cccagcttcc agaattccat cgcgatatca
     3481 ggcagccgcg tcgtccgccg ctcgtcaata gcaagaaacc cccgccgcaa tccttccagt
     3541 cccagaaagt gtcccgatcc ctgatcggcc agcgccggtc cccatcctcc cgcggtcacg
     3601 atctcccccg tcgccgcacg ccccgcgacg ttcgaccccg tccccgccag aaccagcacc
     3661 cccctctgcc caaagaacac cgagtccagt gcaacatcca catcgccctc gatgatcagc
     3721 tctcctccaa catgccgcgc aaacgcatcg cgcagccagt ccaccaccag gggcaccgtc
     3781 tcccccgccg tccccacaca gcaacgcgca acattccccc gctcgacccc cgtcctggcg
     3841 cggagctctt ccagccctcg ccgcaggttg gcttcggccg tctctgcatc cgctttcatc
     3901 cgcttgatgg tgcccgtctg cacccgagcc ac
//
LOCUS       DASZYN010000225         7251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_199745, whole genome shotgun sequence.
ACCESSION   DASZYN010000225 DASZYN010000000
VERSION     DASZYN010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7251
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199745"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="VHE33_05800"
     CDS             complement(<1..1128)
                     /locus_tag="VHE33_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97000.1"
                     /translation="MRSSGSSRISNNRRRALEPVSTLAGPEYVARSLRHEAIFAGLVA
                     ALFSVLAFAYCYPRDMLLLYGDAVAHLHIARRILDSVNPGIRQIGSVWLPFPHLLMMP
                     FAARLVWWQNGIAGAIPSMACYVLSVAGLWRLARYWIRPAGAVVAALFFALNPGLLYM
                     QTTAMGETVFLCQVIWATLFLVDFLRGLGIRSPRGSQSPNGNLDGQSAGRALTACGII
                     LVTAIYTRYDGWIFATLIWLIALRAMFRLRAWNSRAGGAFVLFTAMLVTTPVLWFAWN
                     ARQYGDWLDFMRGPYSAKAIDARTSTPGAPHYPGWHSMRVAALYYIKSAEMGVVPVWW
                     GNRLLFLAVAGTAAAVWKWRKKCLAPFLLLWLPLPFYAYSVA"
     gene            complement(1094..2029)
                     /locus_tag="VHE33_05805"
     CDS             complement(1094..2029)
                     /locus_tag="VHE33_05805"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HVT97001.1"
                     /translation="MAILVVGSVAFDAIETPSGHRDRCLGGAATYFSLAASNFTDVRV
                     IGVVGEDFGPEEESVLTRRKIDTRGIEHVPGKSFFWSGSYMKNLNEAQTLNTELNVFQ
                     SFSPKIPAEYHDSEYLFLANIDPVLQEDVRRKMPKVKMVCGDTMNYWIKDHAENLARV
                     LKGLDVLLINDGEAKMLAKDNNLLAAAHKILDMGPKALVVKHGEYGATAFFGHHSFSG
                     GDGSVTVPFRAPALPLAEVVDPTGAGDSFAGGFFGYIASQATLTPAVFRRAMFYGGVL
                     GSFAVERFGTERLQNLQPKEIEERFALFRQLSHLE"
     gene            complement(2054..2944)
                     /gene="mtnP"
                     /locus_tag="VHE33_05810"
     CDS             complement(2054..2944)
                     /gene="mtnP"
                     /locus_tag="VHE33_05810"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HVT97002.1"
                     /translation="MPARGDSLQQAEIGIIGGSGLYAMPGLTNIHEEHVETPFGAASD
                     SYVLGDLEGRKVAFLARHGRGHRILPSELNFRANIFGMKKLGVERIVSVSAVGSLKEE
                     HKPGDFVVPDQFIDRTFHRISTFFGDGIVAHVAFGDPVCATIAGAIFSACKSEGVEAK
                     SGGTYVCMEGPQFSTRAESNLYRSWGSDVIGMTNLQEAKLAREAEICYATLAMVTDYD
                     CWHEGHDAVTIDQIVKVMNQNSANACRVVKTAVAAMPRERKCACSTALQFAILTSPDA
                     IPEATREKLDLLIGKYLKKN"
     gene            complement(3011..4603)
                     /locus_tag="VHE33_05815"
     CDS             complement(3011..4603)
                     /locus_tag="VHE33_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97003.1"
                     /translation="MQRFINFGCALMALLFVLSTGAQTTANTPVEPPPQAGQAGAQTG
                     EAKTPAPETHITPQQAKELFSSIGPILQFASEDTKLPIRHQVKPHLTTRSEVEKYLLE
                     KMKDDKDAKRMERSEIVLKKFGLIDADFHLQSFLVKLLDEQIAAYYDSKTKTVNLLDW
                     IAPETQKPVLAHELTHALQDQRVNLDKWQSQTSDDLSHSYAEDRRHIASDEDDTAREA
                     VLEGQAMAVFVDYSLAPAHRTLRNSPGIIDNLLDTMTDDGDSPVLSRAPLLLQESLLF
                     PYKDGLRFEAALVADSKNPAAAYGDVLDRPPASSYEIMNPHAYEDEVKAPLLNMPDIH
                     PVIDAQYSPYDIGVMGELDVRILAELFGGKEMAAALTPAWDGGIYYAAQKKDAADKSS
                     TASVSLFYLSQWKSEEAAANFVKMYDDELSRKYTHVSRNSSEESSPGEQVYDTNEGPV
                     LIVTSGKDVFVSESFDLTIARKLQFLMLGAQQTSGQNMALKLAPTFPEVRGSVSGFGL
                     SGGLVQFMARCGMMKAAIHHLY"
     gene            4674..5831
                     /locus_tag="VHE33_05820"
     CDS             4674..5831
                     /locus_tag="VHE33_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97004.1"
                     /translation="MTQTYQNPGAAVLSALEAQNATAGEAWRTPLTSRLSTDDASTPE
                     LTMHRGAETPLRFSATPAELRLLVQKAGVFDLGYRTVLRATGKDRIRWLNGMITQAVK
                     NMTPGQIGYTLVLSPQGRIQGDGDVYCFEDSLLLETDRFQVERLLTHLRRFIIMDDVR
                     LEELDAAETAIGIAGPQTAEILEKLGGSLPEPDTFRRGQLAGIDVTLIRTWSPAVTKA
                     EIRVDAGQVVDLWNALAEAGATPCGTAALEDLRVLEGTPLFDVDFGDKHLPQEANLFR
                     ALNFTKGCYIGQEIVERIRARATVHRSLRQFELHGETPNLNPGETVELRAGEATVGEL
                     TSVARIDLTGVARTFALGVARTEALDKSETQPICYPGGTALPLAMPPVHLG"
     gene            5863..6450
                     /locus_tag="VHE33_05825"
     CDS             5863..6450
                     /locus_tag="VHE33_05825"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HVT97005.1"
                     /translation="MDEKQPTFTVSDKRKFTLEGELRDESQAADLAEEKPAAPANVVT
                     MPAPEERGADPETEGVDELEVPQFSAQETAEQDAAYKESSRQIDDMLRQANPGVGTGG
                     TITFEHIIQSFYVSAMMAMGAGTEPGQKARVDILGARQSIDMLALLQEKTRGNLTPKE
                     EATLQNVLFELRMMFLEITNAIAQQAQKAADPKKK"
     gene            6491..>7251
                     /locus_tag="VHE33_05830"
     CDS             6491..>7251
                     /locus_tag="VHE33_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVT97006.1"
                     /translation="MKAEIVVLGSGTSMGVPTLGCQCAVCTSTDPRNRRTRPSIAVTW
                     NNTGATHLVVIDTGPDFRSQALREGIRHVDAVFYTHAHADHILGLDDLRPLSFAYKPK
                     SLPLYADRPTADILRRVYDYTFSPTSTYPNKARVELRDLAEEQTVNVHGAQFQRVPLL
                     HGDLEVAGFRFGSAAYLTDMNAIPESSLPLLHGLDVMIIDALRPQPHPSHANIAESLR
                     WVERVRPRQAWFTHMSHEVEHDTTEQTLPANVRLA"
BASE COUNT         1506 a         2177 c         2148 g         1420 t
ORIGIN      
        1 ggcaacggag tacgcgtaga acgggagcgg cagccacagc agcagaaagg gcgcgagaca
       61 tttcttccgc catttccaga ctgctgccgc cgttcctgcg acagcaagaa acagaagacg
      121 attgccccac cagactggaa ctacgcccat ctcggcggac ttgatgtagt agagcgcggc
      181 aacccgcatg ctgtgccaac cgggatagtg cggcgcacct ggcgtcgaag tccgggcgtc
      241 aatggctttg gcggagtagg gaccacgcat gaagtcgagc cagtcgccgt actggcgggc
      301 attccatgca aaccagagga ccggggtggt gacgagcatc gcggtgaaga gcacgaacgc
      361 gcccccggcg cggctgttcc aggcccgcaa gcggaacatc gcgcgcaaag caatgagcca
      421 gatgagcgtg gcgaaaatcc agccgtcgta gcgtgtgtag atggcggtga ccagaatgat
      481 gccgcaggcg gtaagggctc gccctgcgga ctggccgtcc aggttgccat ttggggactg
      541 cgatccgcgc ggcgatcgga ttccgaggcc acgcaggaaa tccaccagga agagcgttgc
      601 ccagatgacc tggcacagga agacggtctc gcccatggcc gtggtctgca tgtagagcag
      661 cccgggattc agcgcgaaga aaagcgcagc aacgaccgct ccggcgggcc ggatccagta
      721 acgcgccaga cgccagaggc cggcaacgct gaggacatag caggccatcg acgggatggc
      781 gccggcgatg ccgttttgcc accaaaccag gcgcgcggca aacggcatca tcagcaggtg
      841 ggggaacggc agccagaccg acccgatctg ccgtatgccg ggatttaccg aatccaggat
      901 acggcgggcg atgtgcaggt gcgcaaccgc gtctccgtaa agaagcagca tgtcgcgggg
      961 atagcaatac gcgaacgcga ggacggaaaa cagcgcggcg accaaacccg caaagatggc
     1021 ttcgtgtcgc agagagcgcg caacgtattc cggtccggcc agcgtcgaaa ccggttccag
     1081 ggcgcgccgg cgattattcg agatgcgaga gctgccggaa gagcgcaaag cgttcctcga
     1141 tctccttagg ctgcaggttt tgcaggcgct cggtgccaaa gcgctccacg gcaaacgagc
     1201 ccaggacgcc gccatagaac atcgcacgac gaaagacggc cggtgtcagc gtggcctgtg
     1261 aggcgatgta accgaagaat ccgccggcga acgaatctcc tgccccggtt ggatcgacga
     1321 cttcggccaa tggcagcgcg ggcgcgcgaa acggaaccgt cacgctgcca tcgccgccag
     1381 agaaggaatg atggccgaag aacgcagtag ctccatactc cccgtgcttg accacgagag
     1441 ccttcgggcc catatcgaga atcttgtgag ctgccgcaag caggttgttg tccttcgcca
     1501 gcatcttggc ttcgccgtcg ttgatcagta gaacgtcgag ccctttcagg accctggcga
     1561 gattctccgc gtggtccttg atccagtagt tcatcgtatc gccgcagacc atcttcacct
     1621 tcggcatctt gcgtcggacg tcttcctgca aaacagggtc gatattggcg agaaacagat
     1681 attcgctgtc gtgatactcg gcggggattt tgggagaaaa actctggaat acgttcagct
     1741 cggtattgag cgtctgcgct tcgttgagat tcttcatgta cgaaccgctc cagaagaagc
     1801 tcttccccgg gacgtgctcg atgccgcgtg tgtcgatctt gcggcgagtg agcacgctct
     1861 cttcttccgg accgaagtcc tcgccgacga ctccaatcac gcgaacgtcg gtgaagttgc
     1921 tggccgcgag tgagaaatag gtggccgcgc cgccaagaca acgatcgcgg tgcccggagg
     1981 gagtttcgat ggcgtcaaac gcgacgctgc cgacaacaag aatggccatg ggttggattg
     2041 ttcctcgtgg ttgttagttc ttcttcaggt acttgccgat cagtagatcc agcttctcac
     2101 gcgtggcttc gggaatagca tcagggcttg taagaatcgc gaactgcagc gccgtgctgc
     2161 aggcacattt gcgctcgcga ggcatcgcgg ccacggccgt tttcaccaca cggcaggcgt
     2221 tggccgagtt ctggttcata accttcacga tctggtcgat agtgacggcg tcgtgaccct
     2281 cgtgccagca gtcataatcg gtcaccatgg ccagggtggc gtagcaaatc tcggcctcgc
     2341 gggccagctt cgcttcctgc aggtttgtca tgccgatcac atcactgccc cagctgcgat
     2401 aaaggttgga ttcggcgcgt gtggaaaatt gcgggccttc catacaaacg taggtgccac
     2461 ccgattttgc ctcgacgccc tcgcttttgc aggcgctgaa tatcgcgcct gcgatggtcg
     2521 cacacaccgg atcgccaaat gccacgtgcg cgacaatacc atcgccaaag aaggttgaga
     2581 tgcgatggaa ggtgcgatcg atgaactgat ccggaactac aaaatctccg ggcttgtgct
     2641 cttccttcag cgaccccacc gctgaaaccg aaacaattcg ctccacgccg agcttcttca
     2701 tcccgaaaat attggcgcga aagttcagtt ccgagggcag aatacggtgg ccacgcccgt
     2761 ggcgggcaag gaacgctacc ttgcgtcctt ccagatcgcc cagaacgtac gaatcgctgg
     2821 cggctccgaa aggagtttct acatgctctt catggatatt cgtaaggccg ggcatggcgt
     2881 acagtccgct gcccccgatg atgccgatct ctgcttgttg caatgagtct cccctggcgg
     2941 gcatgctgcc tcaccgcaga gatgagagag ccaaacccgg ataagcagcg cgctggatca
     3001 gcgcaagtga tcagtataaa tggtgaatcg cggccttcat catgccgcat cgcgccatga
     3061 actgcaccag ccctccgctc agaccgaacc ctgagacgga accccggact tccggaaaag
     3121 tgggggcaag tttcagcgcc atgttttgtc cgctcgtctg ctgcgccccc agcatcagga
     3181 actgcagctt acgcgcaatc gtcaaatcga aactctcgct gacaaagaca tctttgccgc
     3241 tggtaacgat caggaccggg ccttcgttgg tgtcgtatac ctgctcccca ggactgcttt
     3301 cttcagacga atttcgcgaa acatgagtat attttcggct cagctcatcg tcgtacatct
     3361 tcacgaaatt cgcggcggct tcttcggatt tccactggga gagatagaaa agcgaaacgg
     3421 acgccgtcga actcttgtcg gcagcgtctt ttttctgtgc tgcgtagtag atgcccccgt
     3481 cccatgccgg tgtcagtgcc gccgccatct ctttcccgcc aaaaagctca gccagaatgc
     3541 gcacatcgag ctcgcccatc accccaatgt cgtatgggct gtattgcgca tcgatcactg
     3601 gatgaatatc gggcatgttc agaagagggg ctttcacctc gtcttcatat gcatgcggat
     3661 tcatgatttc gtagctgctg gccggtggac gatcaaggac atcaccatag gctgcagcag
     3721 ggttcttcga gtcggctacc agcgccgctt cgaacctgag cccgtctttg taggggaaca
     3781 gcagcgattc ctgaagcagc agcggcgcgc gggacaacac cggcgagtcg ccgtcgtccg
     3841 tcatcgtatc cagcagattg tcgatgattc ctggcgaatt gcgcagagtg cggtgcgcgg
     3901 gcgcgaggct gtagtccacg aacaccgcca tggcctgccc ctcaaggacc gcttcccgcg
     3961 cggtgtcgtc ctcgtcgctc gcgatatgcc tgcggtcttc tgcgtaactg tgggagaggt
     4021 catcgctggt ctgactctgc cacttgtcca gattcactcg ctgatcctgc agagcatgcg
     4081 tcagctcgtg cgccagcacc ggtttctggg tttcaggggc gatccaatcc agcagattga
     4141 ccgttttcgt cttgctgtcg tagtacgcgg cgatctgctc gtcgagcagc tttaccaaaa
     4201 atgactgcag gtgaaaatcc gcatcaatga gcccgaactt cttcaggacg atctcggacc
     4261 gctccatgcg tttcgcatcc ttgtcgtcct tcatcttctc gagcagatac ttctccacct
     4321 cgctccgcgt cgtaaggtgc ggcttcacct ggtgccgaat tggcagtttc gtatcttccg
     4381 aggcgaactg caggatcggg ccgatggagg agaacagttc cttcgcctgc tgcggcgtaa
     4441 tgtgggtttc aggagcgggc gtcttcgcct ctccggtttg cgctcctgcc tgccctgcct
     4501 gcgggggcgg ctcaaccggc gtattagcag tggtttgggc gcctgtagag aggacgaaca
     4561 ggagcgccat tagcgcacac ccgaagttga tgaatcgttg cattcccgtt ccctgcgcca
     4621 cctgtcgctg tttcgctgct acctgcagac tataattttt tagagagccg tgtatgactc
     4681 agacatatca aaatcccggc gccgctgtcc tctctgcact ggaagcccag aatgcgacgg
     4741 ctggggaggc atggcgaacg cctctgacaa gccgtctgag cactgacgac gcctcgactc
     4801 ctgaattgac gatgcaccgc ggagcagaga cgccgctccg tttctctgcc accccggccg
     4861 aattgcggct cctggtccag aaagcagggg ttttcgacct tggataccgc accgttctgc
     4921 gcgctaccgg caaggaccgc attcgctggc tgaatggcat gattacgcag gccgtaaaaa
     4981 acatgacgcc cgggcaaatc ggttacaccc tggttctcag tccgcagggt cgaattcagg
     5041 gcgatggtga tgtctattgt tttgaggatt ctctactcct ggagacggac cgctttcagg
     5101 tagagcgtct cctgacccat ctgcgccgct tcatcatcat ggatgacgtg aggctcgaag
     5161 aattagacgc tgcagagacc gcaattggca tcgcaggacc ccaaaccgcc gagattctgg
     5221 agaagctggg tggtagcttg ccggagccgg atacgttccg gagaggacag ctcgccggca
     5281 tcgatgtcac gctgatccgc acctggagtc ctgcggtcac aaaagccgaa atccgcgtgg
     5341 acgccgggca ggtcgtggac ctctggaacg cactggctga ggcgggcgca actccctgcg
     5401 gtaccgcggc tcttgaagat ctgcgtgtgc tcgagggaac gccgctgttc gatgtcgatt
     5461 tcggcgataa gcacctgccc caggaagcga acctgttccg cgccctgaac tttaccaagg
     5521 gttgctatat cggccaggag attgtcgagc gtattcgcgc acgggccact gtccatcgct
     5581 ccctgcgaca gtttgagctg cacggggaga cacccaacct caatcccgga gagacggtcg
     5641 aactgcgcgc cggtgaagca acggtgggcg aattgaccag cgtggcgcgg atcgacctga
     5701 ccggagtggc cagaactttt gcgctcggag tggcgcgtac tgaagccctc gataagagtg
     5761 aaacccaacc gatctgctac cctggcggga cggccctgcc cctggccatg ccgcctgtcc
     5821 accttgggtg atcggtgatt tttgcggaga gaaacgtaat ttatggatga gaaacagccg
     5881 acgttcacgg taagcgacaa gcgcaaattc accctggaag gtgagttgcg cgatgagagc
     5941 caggcggccg atttggccga agagaaacct gccgcacccg cgaatgtcgt cacgatgccg
     6001 gcacccgagg agcgtggggc cgatccggaa accgaaggcg tcgacgaact cgaagtgccg
     6061 cagttttccg cgcaggaaac tgcggaacag gacgctgcct acaaggaatc atctcggcag
     6121 atcgatgaca tgctgcggca ggcgaacccc ggggtgggaa ctggcggaac aatcaccttc
     6181 gagcacatca tccagtcgtt ctacgtgtca gcgatgatgg ccatgggcgc agggaccgaa
     6241 cctggccaga aggcgcgcgt cgacattctc ggtgcccggc aaagcatcga catgctggcc
     6301 ctgctgcagg agaaaaccag ggggaatctc acgccgaagg aagaggcgac gctgcagaat
     6361 gttctcttcg agctgcgcat gatgttcctg gagatcacga acgcgattgc gcagcaggcc
     6421 cagaaagccg ccgatccaaa aaagaagtga tccccctttc tcatccaacc tgtctgatga
     6481 atccagccgc atgaaggcgg aaatcgttgt tctcggcagc gggacctcca tgggggtgcc
     6541 gacgctcggc tgccagtgcg ccgtatgcac ctctaccgat ccacgcaatc gcagaacccg
     6601 cccctcgatt gccgtcacct ggaacaatac cggtgcgacg catttggtgg tcattgacac
     6661 aggtccggac ttccgcagcc aggcactgcg tgaaggcatc cggcacgtgg atgccgtgtt
     6721 ctatacgcac gcccatgccg accatatcct cggtctcgac gatctgcgtc cgctgagctt
     6781 cgcctacaag cccaaatccc tgcccctata tgcagatcgg ccaacagccg atattctccg
     6841 tcgggtctat gactatacct tctcgcccac ctcgacgtat cccaataagg cgcgcgttga
     6901 gctgcgcgat ctggcagagg agcagacagt caatgtgcac ggggcgcaat ttcaacgggt
     6961 accgctcctg cacggtgatc tcgaggtcgc ggggtttcgc tttggatcgg cggcctacct
     7021 gaccgatatg aacgccattc ctgagtccag tctgccgctg cttcacgggc ttgacgtgat
     7081 gatcatcgat gccctccggc cccagcccca ccccagccac gccaacatcg cagagtcgct
     7141 gcgatgggtc gagcgcgttc gcccccggca ggcctggttc acccatatgt cgcacgaagt
     7201 ggagcacgac accaccgagc agacacttcc agccaatgtg cggctcgcct g
//
LOCUS       DASZYN010000226         4970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_970706, whole genome shotgun sequence.
ACCESSION   DASZYN010000226 DASZYN010000000
VERSION     DASZYN010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970706"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VHE33_05835"
     CDS             <1..511
                     /locus_tag="VHE33_05835"
                     /inference="COORDINATES: protein motif:HMM:NF018652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97007.1"
                     /translation="RIAEFYTEAIGFLAGHGLAQYEISNFARTGCESRHNLKYWRRQP
                     YLGFGLDAHSMLRTSARVPLRFATTDDLDVYLKDSGWETPRRLDREEELEEAWFLGLR
                     LNEGVSLGALRQEFSSSAVREFLSTIAELEDEGLITFAEGDRVALTARGRLLSNDVFE
                     RFLAEPAVA"
     gene            547..1629
                     /locus_tag="VHE33_05840"
     CDS             547..1629
                     /locus_tag="VHE33_05840"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HVT97008.1"
                     /translation="MSSGTAAVAEHDTAASAQVAMIDLRSDTVTKPTPAMRAAMAAAE
                     VGDDVYGEDPTVNRLEARAAEIFGREAALFVPSGTMGNQTAIRLNTQHGQEVICEARS
                     HIVEWEIAMASAFSGCQLRTIPADRGILTWSAMAPHITGKHYHRAPTGLIALENTHNM
                     AGGTVTPLEVTQEILAGAKERGLPVHLDGARIFNASVALGVGVRELTRDFTTVMFCLS
                     KALGAPVGSMLVGTFDQMDRARLIRKALGGGMRQAGVLAAAGLIALEEGPKHLQADHA
                     NARLLADAVAQAGAPIDLSTVQTNIVIFRVPDGNADAFSAKLKKRGILASSIYGSSIR
                     FVTHRDVDRAACEEAARITVELLGNG"
     gene            1690..2676
                     /locus_tag="VHE33_05845"
     CDS             1690..2676
                     /locus_tag="VHE33_05845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVT97009.1"
                     /translation="MPKYSIVVPFHNEEESVTALYDRLKDVMEHVGESFELVFVDDGS
                     NDRTCRLLEEIAAVDSRVLVVKLRRNFGQTSALAAGFDHAEGEFILAMDGDLQHDPGE
                     IPAFIEKLEEGYDVVSGWRKERIDSFVMRRIPSRCANWLMAKLSGVDIHDFGTTFKAY
                     RREVIHNIPLYGEMHRFIPALASWYGASICEVPITNVNRTVGKSHYGISRTFRVFFDL
                     MTIRFLLKYMTRPLHFFGLFGALGIVGGSGLSMFLLALKVFTHQHVMDEHGPLFVVAA
                     VMILAGIQLLALGLLGELQVRHYHAAAQRAPYTVDRLVRLRASEESNILPDN"
     gene            2834..4828
                     /locus_tag="VHE33_05850"
     CDS             2834..4828
                     /locus_tag="VHE33_05850"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HVT97010.1"
                     /translation="MQKPADGRKRVVIESVDPEIDGGRYAVKRIIGDSVIVEADVFAD
                     GHDHVAAHLLFRFDENSEWASMPMQPLGNDRWRAEFPVTRMGEYVYTVAGWIDHFDTW
                     HSDLEKRIAAGQDIRVDLLNGAQLVEQAAERAGRDDADALRRWATQLRATDDVEVAQT
                     AALDPALAIMMAQYLDPAMETRYERDLRVTVDREKARYSAWYELFPRSTATEPGRHGT
                     FKDCEERLDYIARLGFDVLYLPPIHPIGQAFRKGKNNSTTAESGDTGSPWAIGAKAGG
                     HTAIHSKLGTLEDFRHLLRAAADKGIEMAMDIAFQCSPDHPWVTEHPAWFKHRADGTI
                     QYAENPPKKYQDIYPLDFESSDWQGLWDGLRDVFRFWCEQGVRIFRVDNPHTKAFPFW
                     EWCIAELKRDYPDALFLAEAFTRPRVMQRLAKLGFSQSYTYFTWRNTKQELTDYFTEL
                     TRTRVREYMRPNLWPNTPDILHETLQVGGRPAFLSRIVLAATLGPNYGIYGPAYELLE
                     NTPVRPGSEEYLNSEKYQIRDWDLAAPHSIAGTIANINRARRENPALHANHDLHFHST
                     DNPYLIGYSKSSIDRGNLVLTIVNLDSFHRQSGWIDLDLGQLSLRPDESFQLLDELTG
                     ARYVWQGSRNYIELDPGRIPAHIFRILRKVRSEKDFDYYL"
BASE COUNT          967 a         1510 c         1490 g         1003 t
ORIGIN      
        1 tcgcatcgct gagttttata ccgaagcgat cggctttctt gccggccatg gcctggcgca
       61 atacgagatc tcgaattttg cccgcaccgg ctgcgagtcg cggcacaacc tgaaatactg
      121 gcgccgccag ccctatctcg gctttggcct cgatgctcac tccatgttgc ggacgagtgc
      181 ccgcgtcccg ttgcgattcg caacaacgga cgatctggat gtttatctga aggattcggg
      241 atgggaaaca ccgcgccgcc tcgaccggga ggaagagctg gaggaggcct ggttcctcgg
      301 tctgcgtctg aacgagggtg tcagtcttgg cgcgctgcgt caggagttct cgtcatccgc
      361 ggtccgcgag tttctgtcca ccatcgcgga gctggaggac gaaggtctga tcacgttcgc
      421 cgagggcgac cgggtcgcac tgacggcacg cggacgtttg ctctcgaacg atgtctttga
      481 gcgattcctg gcggaacccg ccgtcgccta ggtcatactc aaaccagttg atgtcgaggg
      541 ggaatcatgt cttctggtac ggcagccgtg gccgaacacg ataccgccgc ttccgcgcag
      601 gtcgcaatga tcgacctgcg cagcgacacg gtgaccaaac ccacgcccgc gatgcgtgcg
      661 gcaatggccg ccgccgaagt gggcgatgac gtttatggcg aggaccctac cgttaaccgg
      721 ctggaagcgc gggcggcgga aatcttcggg cgcgaagcgg ccctgttcgt gcccagcggt
      781 accatgggca atcagaccgc gatccggctg aatacgcagc acggccagga agtcatctgt
      841 gaggcccgtt cgcacatcgt ggagtgggag atcgcgatgg cttcggcgtt ctccggatgc
      901 cagctgcgaa cgattcctgc cgaccgcggc attcttacct ggagcgccat ggcgccacac
      961 atcaccggca agcattatca ccgcgcgccg acgggcctga tcgcgctcga gaacacgcat
     1021 aatatggcgg gcggcaccgt cactccactc gaagtgacgc aggagatcct cgccggggcg
     1081 aaggagcgcg ggctccccgt ccacctcgac ggtgcgcgca tcttcaatgc gtccgtcgcg
     1141 ctgggagtcg gcgtgcgcga actcacccgc gacttcacca cggtgatgtt ttgcttgtcg
     1201 aaagcattgg gagccccggt gggatcgatg ctggtgggca cattcgatca gatggatcgc
     1261 gcgcggctca tccgcaaggc cctcggtgga ggcatgcgcc aggccggcgt tctggctgca
     1321 gccggattga tcgcactgga ggaaggcccg aagcatctgc aggccgatca tgccaatgct
     1381 cgcctgctgg ccgacgccgt ggctcaggcg ggcgcaccca tcgacctgag caccgtgcag
     1441 acgaacatcg tcatcttccg cgttccggat ggcaatgcag atgcgttctc tgcgaagctt
     1501 aaaaagcgcg gaatcctcgc cagttccatc tatgggagca gcatccgttt tgtgacgcac
     1561 cgcgacgtgg accgcgccgc atgcgaggaa gcggctcgga tcacggttga actgctcggc
     1621 aatgggtaaa cctttaagga tgagtccaat cacgggttcc gtgcatccaa aagactataa
     1681 ttgacgttca tgccgaagta ttccatcgtc gttccgtttc ataacgaaga ggagagcgtc
     1741 accgctctct atgaccgtct caaggacgtc atggagcacg tgggcgaatc gttcgagctg
     1801 gtttttgttg atgacggctc caacgatcgc acctgtcgcc tgctggaaga aattgccgcc
     1861 gtcgacagcc gtgtcctggt ggtgaagctg cgccgcaatt tcggtcagac ctctgccctg
     1921 gcggcagggt tcgatcacgc cgagggcgag ttcatcctgg cgatggatgg cgatctgcag
     1981 cacgatcccg gcgagattcc cgccttcatc gaaaaactcg aagagggtta cgacgtcgtc
     2041 agcggatggc ggaaggaacg catcgatagt ttcgtgatgc ggcgcatccc ttcgcgttgc
     2101 gccaactggc tcatggcgaa gctgtcggga gtcgatatcc acgatttcgg cacgacgttt
     2161 aaggcctatc ggcgcgaggt catccacaat attccgcttt acggcgaaat gcaccgattc
     2221 attccggctc tggcctcgtg gtatggcgca agcatctgcg aagttcccat caccaacgtc
     2281 aaccgcaccg tcgggaagtc gcactacgga atcagccgca cgttccgggt cttcttcgac
     2341 ctgatgacga tccggttcct cctgaagtac atgacccgcc ccctgcattt cttcgggctt
     2401 ttcggcgccc tgggcatcgt gggcggcagc ggcctgtcca tgtttctgct ggcgctgaag
     2461 gttttcacgc atcaacacgt gatggatgag cacgggcccc tgttcgtggt ggctgcggtc
     2521 atgatcctgg cgggaattca gttgctggcc ctcggtctgc tcggcgaact gcaggtgcgc
     2581 cattaccacg cagcggctca gcgcgctccg tatacggtcg atcggctggt gcggctgcgc
     2641 gcgtcggaag agtcgaatat cctgccggac aattaatcgc cggtaccttt cgcggcaacc
     2701 taacttgttg catctcctat cccagtagcg agaatttgct ttttgtcttc gtataatttc
     2761 gctagctgac acgcgcgaga agtcgtcact tgttgcgcgc gggccactgc cagcaggaga
     2821 gccaatcgga gccatgcaga agcccgcgga cggacggaaa cgcgttgtca tcgagagcgt
     2881 cgatcccgag atcgatggcg gccgttacgc tgtcaagcgc atcatcggcg actccgtcat
     2941 cgttgaggca gacgtctttg ccgatgggca cgatcacgtt gcagcgcacc tgctgttccg
     3001 ctttgatgag aattccgaat gggcctccat gcccatgcag ccgttgggca acgatcgctg
     3061 gcgagcggag tttccggtta cccggatggg cgagtacgtc tataccgtcg ctggctggat
     3121 cgatcatttt gatacctggc acagcgacct cgagaagcgc atcgccgccg ggcaggatat
     3181 ccgcgtggat ctcctgaacg gcgcccagct ggtggagcag gccgccgaac gcgccgggcg
     3241 cgacgatgcc gatgcgctgc gacgctgggc cacgcagttg cgagctacgg acgatgtcga
     3301 agtggcgcag accgccgcgc tcgacccggc actcgccatc atgatggctc agtatctcga
     3361 tcccgccatg gaaacgcgct acgaacgcga tctgcgggtg accgtcgatc gtgagaaggc
     3421 ccgttactcg gcctggtacg aattgttccc gcgctccacg gcaaccgaac cgggacgtca
     3481 tggaaccttc aaagactgtg aagagcgcct cgactacatt gcgcgcctcg gattcgacgt
     3541 tctctatctg ccgcccattc accccatcgg ccaggctttt cgcaagggca agaacaactc
     3601 gaccaccgct gaatccggag atacgggcag tccctgggcg attggtgcga aagccggggg
     3661 gcatactgcg attcactcca agctgggcac gctcgaagat tttcgtcacc ttctgcgcgc
     3721 ggccgcggat aagggcatcg agatggcgat ggatatcgcc tttcagtgct cgccggatca
     3781 tccctgggtc accgagcatc cggcatggtt caaacatcgc gccgatggaa cgatccagta
     3841 cgcggagaac ccgcccaaga aatatcagga catctatccg ctcgattttg aaagcagcga
     3901 ctggcagggc ttgtgggatg ggctgcgcga tgtgtttcgc ttctggtgcg agcagggtgt
     3961 ccgcatcttt cgcgtcgaca acccccacac gaaggcgttt cccttttggg agtggtgcat
     4021 cgcagagctg aagcgcgact atcccgatgc gctgttcctg gcagaagcct tcacgcggcc
     4081 gcgtgtgatg cagcgtctcg ccaagctggg attctctcag tcctacacct acttcacatg
     4141 gcggaatacg aagcaggagc tcacggacta tttcaccgaa ctgacgcgaa cccgggtgcg
     4201 cgagtatatg cgccccaatc tgtggcccaa cacgccggac atccttcacg agacgctgca
     4261 ggtgggggga cggcccgcat tcctcagccg cattgttctt gctgcgacgc tcggacccaa
     4321 ctacggcatc tacgggcctg cctatgagct tctggaaaac acgccagtgc gccccggcag
     4381 cgaggagtac ctgaactcgg agaagtatca gattcgggac tgggacctgg ccgcgccgca
     4441 cagcattgcg ggcaccatcg cgaatatcaa tcgggcacgc cgagagaatc cggctctgca
     4501 tgccaaccat gacctgcatt ttcattcgac cgataatccg tatctgattg gctacagcaa
     4561 atcgagcatc gatcgcggca atctcgtcct taccatcgtg aacctcgatt cgttccaccg
     4621 gcagtctggc tggatcgacc tcgacctcgg ccagttaagc ctccgtcccg atgagagttt
     4681 tcagcttctc gatgaactaa cgggcgcccg ctatgtatgg cagggatccc ggaactacat
     4741 cgaactcgat cccggcagga ttcccgcgca catcttccgc attctgcgca aggtgcgttc
     4801 cgaaaaggat ttcgactact atctgtgagc ccacaggatt ccatgccgga ttcgaaagcc
     4861 atcattcgca aaccgggcag cgttaccgat ccgctttggt ataaagacgc gatcatctac
     4921 gaagtccacg tcaaggcgtt ttgcgacagc aacaacgatg gcatcggcga
//
LOCUS       DASZYN010000227         8457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1036403, whole genome shotgun sequence.
ACCESSION   DASZYN010000227 DASZYN010000000
VERSION     DASZYN010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8457
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036403"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VHE33_05855"
     CDS             <1..415
                     /locus_tag="VHE33_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97011.1"
                     /translation="VSAFHDYLTRELKYETTDTYRVHTESEVPWDMHHQMPGQRFGGG
                     GGGRASLPYLAGDLADAMRKNPKLKVFSANGYFDLATPFFVTEYDLAHMMLKPDLAKN
                     VSFGYYPAGHMVYLNVEALKQFKHDMTDWYASTGH"
     gene            complement(569..3802)
                     /locus_tag="VHE33_05860"
     CDS             complement(569..3802)
                     /locus_tag="VHE33_05860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HVT97012.1"
                     /translation="MTRYVELHAASAFSFLEGASQPEDLIERAMELEIPAVALLDRNG
                     FYGSARMHTSAERNSLRAHVGAEISTSAFGPRLTHAPWLPHPRHPDQSAIPEPARLPL
                     LCANRTGYQNLCQLITHFKMRETTKAEGAATFDELAQYSAGLLCLTGGDEGPLAAALA
                     SGGEPAAQALLDRLVAIYGRGNVVVELQRHQVPDQEARNQAALRLARSMRLPIVATNG
                     VRYAAPHDREILDVFTAIRHHVDLDHAGRLLAVNNHRYLRSPAEMSTIFRDLPEAIAN
                     THEISERLQFRLADLGYEFPHYPVPDGDTMDSFLRKRVDEGVRLRYGAKKGSSILDRA
                     RKQVDRELTLIAKLGFAGYFLIVWDIVRYCKQNDILIQGRGSAANSAVCYALEITAVD
                     PIGMDLLFERFLSENRGEWPDIDLDLPSEEKREQAIQYVYQRYGELGAAMTANVITYR
                     GKSAAREVGKALGFDQESLGRLTSLVSQWEWRGENDTMANSFNHAGFDIAHPRIAKYL
                     QLCVRLQDLPRHLGQHSGGMVICQGQLNHIVPLERASMPGRTVIQWDKEDCADLGIIK
                     VDLLGLGMMAVLKDCLTLIPEHYGEPIDLAHLPDDSNVYRVLQRADTVGMFQIESRAQ
                     MASLPRNYPELFYDLVVQVAIIRPGPIVGKMMHPYMRRRQGKEEVTYPHPSLEPVLSR
                     TLGVPLFQEQLLRIAMTVANFTGAEAEELRRAVGMRRSWQRMKDLEVKLRQGMTDNNI
                     PPKTQDEIVQAISSFALYGFPESHAASFALLAYASAYFKVKYLAAFTAAILNNQPMGF
                     YSPAVLVKDAQRHGLKIHSIDVQVSDWACTIEHEANGSLSMRLGLGYARGLRQQAGEA
                     IMASRRTGGAFNSVDDLALRVPELNKKEISLLANIGALNNVAGIGHRRDALWQVERAG
                     KPEGPLLTHTGALLADPQGLSPLAQMTPEERLVADYSGTGLTTGRHPMSYRRAELRRQ
                     NILSAEELRTRRDGEWVRAAGCIIARQRPGTAKGFVFLSMEDETGIANVIVTPQMYES
                     NQVTVTRARFLFVEGPLQNQDNVIHVKAMRLTPLSDNAIEVRSHDFH"
     gene            complement(3795..5435)
                     /locus_tag="VHE33_05865"
     CDS             complement(3795..5435)
                     /locus_tag="VHE33_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HVT97013.1"
                     /translation="MTPPTLYACLYVREFPAQARLRLRSQIGKTPVVILEGTPPRQTV
                     CSCNARARDLGIETGLTRAELDVFSGVTHLLRSLSEESCAYAALLQCAGRFSPRVECL
                     TPQLASDKSSAMSDAASDTYSFAAILDITGTEKLIGPPPILAKKLLAAVRALGLHAAI
                     VISANIDAALCLARSLGSRTTASATSSPVSGWSTAVIPTGDEATALAPLPIFVLPLTA
                     DHAETLTRWGIFTLGALAALPEKELIARLGQHGLRLRQLARGEAPHLLVPVDPPFNLE
                     ERTELEAPVELLDSLLFVLGVLLDQLIARAAAQILALASVTVTLVLDGGSTHTRTVRP
                     ALPGNDRRLWLRLLHLDLQAHPPSAAILAIAVAAEPGSTSKVQQGLFTPQLPEPDRLD
                     VTLARIRSLVGDNRVGRAVLEDTHRPDAFRVERFSIDETRPTTPTATAAATLPVAQKP
                     AHTARRRLRPPEPITVTLSERRPVALVFRNQRYTVERAWGPWFFSGNWWGIECWSMEK
                     WDLVARSDGDAGILSCAIARNPLRPATLNDWRLEEFYD"
     gene            complement(5432..6520)
                     /locus_tag="VHE33_05870"
     CDS             complement(5432..6520)
                     /locus_tag="VHE33_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97014.1"
                     /translation="MPTAAALRQQIEAVLADRIPSALTPPPRVLCPNAPTGLTALDDA
                     LQGGLPIGAITELTGPECSGRTSLALTFLACLTNDNRVCAWVDVSDALHPESAAGAGI
                     DLNRLLWIRCGTEPEQLTLPGMSDKQSDMFFEKQRLAVRNDLATAKSQPSFTDRDSPP
                     IRHDYLAPRCCEPVPKVRRHGREVVPQPVTRNLSPGQPNSQLQTPNLAPQRSRKKPWS
                     RLDQALRATDLLLQGGGFSAIVLDLGSIAPEFALRVPLATWFRYRAAAERTHASVLLL
                     TQHPCAKSSAGLVLHCQPSQIDPDVTTVFTGAEHRVEIVRDRFARVSNVVDFGSRKPP
                     QSDHSILWNTCTPWSTRATPQRPKAGHP"
     gene            complement(6624..7355)
                     /locus_tag="VHE33_05875"
     CDS             complement(6624..7355)
                     /locus_tag="VHE33_05875"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HVT97015.1"
                     /translation="MMKRTLSLALAVLFGAVVASAQTIQVDKNNRTIAVTASDKASAE
                     ADTAVVSVGFQIYAPDAAAAYSKGSAVSNAILAALKKAGVADKAIESQEQNLGKTEFP
                     YNPQPAPEERIQKAFTLSQSWAVHTAARDAAMVLHVAIEAGANQSGNIAWDVTDRKGL
                     QAQAAQKALVDAHAIAAQMASGLGVSLGALVYASNQAPAQPTPPRVYDFMGAAAKAAP
                     PPSPLAIRPQQIEESATVYAVFAIE"
     gene            7497..8276
                     /locus_tag="VHE33_05880"
     CDS             7497..8276
                     /locus_tag="VHE33_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT97016.1"
                     /translation="MALETIDAAAKRGVLALQDYALLSARALGDIFRRPFYRSDIFMQ
                     ADIIGVGSLPIVILTGFFLGGVLALQSAATLSQFGAAAFTGQLVAFSMIKEIGPVVTA
                     LMVSGRNASGMASELGSMIVTEQIDAMRALGTDPLKKLVMPRIVATVVMLFFLVIISD
                     AVGICGGGFVSVFMLGQDGSQYFHTSYQSLEYPDILQGLVKPIFFGFIIATIGCFYGM
                     KTRGGTQGVGRSTTQAVVWSSILIIACDFMISRVMIGIFGR"
BASE COUNT         1627 a         2268 c         2893 g         1669 t
ORIGIN      
        1 tgtcagcgcc ttccatgact acctgacgcg ggagctgaaa tacgagacca ccgacactta
       61 ccgggttcac accgaatcgg aagtgccctg ggacatgcac caccagatgc ccgggcagcg
      121 tttcggcgga ggcggcggcg gacgggcttc cctgccctat ctggcgggcg acctcgccga
      181 tgccatgcgt aagaacccga agctgaaggt cttctcggcg aatgggtact tcgatctggc
      241 aacgcccttc ttcgtcactg aatatgacct ggcgcacatg atgctcaaac ccgacctggc
      301 gaaaaatgtg agcttcggct actaccccgc cggacacatg gtgtacctca acgtcgaggc
      361 cctgaagcag ttcaagcacg acatgactga ctggtacgcg tccacaggcc attgataatc
      421 aggggcgtcc cgagccgtcc ggacgcccct cgtttcctga accgttgttt ccctctcctg
      481 ttacttcctg agacttctat tgttgccggc ggggcatctc cggcaagtgc ggtaatcgct
      541 gaagctcttg cttcggatcc tgatcgcgtc aatggaagtc atgggagcgg acctcgattg
      601 cgttgtcgct aagtggcgtc agacgcatgg ctttcacgtg gatgacattg tcctggttct
      661 gaagaggacc ttcgacgaac aggaagcggg cacgggtaac ggtaacctga ttgctctcgt
      721 acatctgcgg cgtgacgata acgttggcga tgcccgtctc atcttccatc gacagaaaca
      781 cgaagccttt ggctgtgcca gggcgctggc gggcaatgat gcagcccgca gcgcgcaccc
      841 attcgccgtc gcgacgggta cggagttctt cggctgagag gatgttctgg cggcggagtt
      901 cggctcggcg gtaggacatg gggtgacggc cagtggtgag acccgtaccg gagtagtccg
      961 cgacgagacg ctcttcaggg gtcatctgcg caagaggtga gagaccctgt ggatcggcga
     1021 ggagcgctcc cgtatgggtg aggagcggac cttcgggctt gcccgcgcgc tcaacctgcc
     1081 agagcgcgtc gcgacggtgg ccgatcccgg cgacattgtt gagcgctccg atgttcgcga
     1141 gcagggagat ttcttttttg ttgagttctg gcacgcgcag cgcaagatca tcgacagaat
     1201 tgaacgcgcc tcctgtgcgg cgtgaagcca tgatcgcctc tcctgcctgc tggcgaagtc
     1261 cacgagcgta acccaaccca agccgcatcg acaacgatcc attggcttca tgctcgatcg
     1321 tgcaggccca gtctgaaacc tgaacatcga tggaatggat cttcaggcca tggcgctgcg
     1381 catctttaac gagcacagca ggcgagtaaa aacccatagg ctggttgttc aggatcgcag
     1441 cagtaaacgc tgcgaggtat ttcaccttga agtaggccga tgcatacgca aggagtgcga
     1501 agctggcggc gtgggactcc gggaatccgt atagcgcgaa ggaactgatg gcctggacga
     1561 tttcgtcctg cgttttcggc gggatgttgt tgtcggtcat gccttggcgg agtttgactt
     1621 cgagatcctt catccgttgc catgagcggc gcatgccgac ggcgcggcgc agctcttctg
     1681 cttcggcgcc ggtgaagttc gcgaccgtca tcgcaatgcg caaaagctgt tcctgaaaaa
     1741 gcggcacacc cagagtgcgg ctgagcaccg gctcgagtga aggatgcggg tatgtgactt
     1801 cttccttgcc ctgtctgcgg cgcatgtagg ggtgcatcat ttttcccacg atggggccgg
     1861 ggcgaataat agcaacctgc acaaccagat cgtaaaagag ctcagggtag ttgcgcggaa
     1921 gcgaggccat ctgcgcgcgg ctttcgatct ggaacatgcc gacggtgtcg gcgcgctgca
     1981 gaacccggta gacattggaa tcgtcaggca ggtgggcgag atcgatgggt tcgccgtagt
     2041 gctcgggaat gagcgtaagg cagtccttga ggacggccat catgccgagg cccagaagat
     2101 cgactttgat gatgccgagg tcggcgcagt cttccttgtc ccactggatc acggtgcgac
     2161 cgggcatgga ggcgcgttcg agggggacga tgtgattgag ttggccctgg cagatgacca
     2221 tgccgccgga gtgctgaccg agatgtcgcg gaagatcctg tagacggacg cagagctgaa
     2281 ggtatttggc gatccgcggg tgggcgatgt cgaagccggc gtggttgaag gaatttgcca
     2341 tggtgtcgtt ctcaccgcgc cactcccact gactcacaag cgaggtgagg cggccgagag
     2401 attcctggtc gaagccaagc gctttaccga cttcgcgcgc ggccgacttg ccgcggtagg
     2461 tgatgacgtt ggcggtcatg gcggcgccga gttcgccgta gcgctggtag acatactgaa
     2521 tggcctgctc gcgtttttct tccgaaggca gatcaaggtc gatgtcgggc cactcgccgc
     2581 ggttttcgct gaggaagcgc tcgaagagca ggtccatgcc gatgggatcg acggcggtga
     2641 tttcgagtgc atagcaaaca gctgaattgg cggcggaccc acggccctgg atgagaatgt
     2701 cgttctgttt gcagtagcgg acaatgtccc aaacaatgag gaagtagccg gcgaagccga
     2761 gcttcgcgat gagcgtcagc tcgcggtcga cttgtttccg tgcccggtcc aaaatgctgg
     2821 accctttttt tgcaccgtag cggagccgga ccccttcatc gacacgcttg cggaggaagg
     2881 agtccatggt gtcaccatcc gggaccgggt agtgagggaa ttcgtagccg agatcggcaa
     2941 gcctgaattg caggcgctct gagatttcgt gagtgttggc gatcgcttca ggaagatcgc
     3001 gaaaaatggt ggacatttcg gctggcgagc gaaggtagcg atggttatta acagcaagga
     3061 ggcgcccggc gtggtcgagg tcgacatgat ggcggatggc cgtgaagacg tcgagaatct
     3121 cgcgatcgtg cggcgcagca tagcgaacgc cgttggtggc gacaatggga agccgcatcg
     3181 aacgggcgag acggagcgca gcctgattgc gggcttcctg atccgggacc tggtggcgct
     3241 gcaactcgac gacgacattg ccgcggccgt agatagcaac aaggcgatcg agaagggcct
     3301 gggccgcggg ttctccgcca gatgcgaggg ctgcggccag tggaccttca tcgccgccgg
     3361 tgaggcaaag aagtccggcg gagtactgag ccagctcgtc gaaggtcgcc gcgccttcgg
     3421 ctttggtggt ctctcgcatt ttgaaatgcg taatcagctg gcaaagattc tgatagcccg
     3481 tgcgatttgc gcagaggagt ggcaggcggg caggttcagg gatcgcggat tggtcaggat
     3541 gtcttgggtg cggaagccac ggtgcatgcg tcagccttgg gccgaaggca gaggtagaga
     3601 tttcggcgcc gacgtgagcg cggagactat tacgctcggc tgaagtgtgc atgcgcgcgg
     3661 agccgtagaa gccattgcgg tcgagcagag cgacggcggg aatttcgagt tccatcgcgc
     3721 gctcgataag gtcctcgggc tgggaggctc cctcaagaaa ggaaaaggcg gaggcagcgt
     3781 gaagctcgac atacctagtc atagaactcc tccagacgcc agtcgttcag agtcgcaggg
     3841 cgaagaggat tgcgagcaat ggcgcaggag aggatcccag cgtcaccatc ggagcgagcg
     3901 acgagatccc acttctccat cgaccagcac tcgatgcccc accagttgcc ggaaaagaac
     3961 cagggtcccc aggcgcgctc gaccgtgtag cgctggttgc ggaagacaag ggcaaccggt
     4021 cgtcgctcgc tcagcgtgac agtgattggt tccggcggac ggaggcggcg gcgcgcagta
     4081 tgggctggct tttgcgcaac cggcagcgtc gctgctgcgg tagcggtcgg ggttgtgggg
     4141 cgcgtttcat caatcgaaaa gcgctccaca cggaaggcat cgggccggtg cgtgtcttcg
     4201 agaaccgcgc gtccgacacg attgtcgccg acaagcgagc ggatgcgggc gagggtgacg
     4261 tcgagacgat cgggctcggg gagttgcggg gtaaacaggc cttgctgcac tttgctcgtg
     4321 gaaccgggtt cggccgcgac ggcgatagcg aggatagcgg cagagggcgg atgcgcttgc
     4381 agatcaaggt gaagcagacg gagccagaga cgtcggtcgt tgccaggcag agcaggccgc
     4441 acggtacgcg tgtgagtgct gccgccgtcg aggacgaggg tgacggtgac cgatgcgagc
     4501 gcgaggattt gcgcggcggc acgggcaatg agttggtcga gcaggacccc gagaacgaag
     4561 aggagtgagt cgaggagttc gacaggggcc tcgagttcgg tgcgctcttc aaggttaaac
     4621 ggcggatcga cgggtacgag taggtgcgga gcttctccgc gagcaagttg gcgaaggcgg
     4681 agaccatgct ggccgaggcg agcgattagc tctttttctg gcagggctgc aagggcgccg
     4741 agggtgaaga tgccccagcg ggtaagtgtt tctgcgtgat cggccgtgag ggggaggacg
     4801 aagatcggca gtggagcaag ggcagtggct tcatcgcctg tggggatgac agcagtggac
     4861 catccggaga ccggggaaga ggtagcggac gcggtagttc gtgagccgag acttcgtgca
     4921 aggcagagcg cagcgtcgat gttggcactg ataacaatgg cggcgtggag gccgagagcg
     4981 cggacagcgg cgaggagttt tttggcgagg atggggggag ggccgatgag cttttcggtg
     5041 ccggtgatgt cgaggatcgc ggcgaaggag tatgtgtcgg acgccgcgtc tgacatggcg
     5101 ctggatttgt cggacgccaa ttgcggcgtc agacattcaa cacgaggcga gaaccggccg
     5161 gcgcactgga ggagagcagc gtaggcgcac gattcttcgg atagagatcg cagcagatgg
     5221 gtgacaccgg agaagacatc gagttcggcg cgagtaaggc cagtttcgat ccccaggtca
     5281 cgagcacgtg cgttgcacga gcagaccgtc tgccgcggtg gagttccttc aaggatgacg
     5341 accggagttt tcccgatctg agagcgaagg cggagcctgg cctgcgcagg aaattcacga
     5401 acatagaggc aggcatagag cgtgggaggg gtcatgggtg gcctgctttc ggtcgctgag
     5461 gggtggctcg ggtcgaccac ggcgtgcagg tgttccaaag gatcgagtgg tcactttgcg
     5521 gaggcttgcg gctaccgaag tcgacgacgt tgctgacgcg ggcaaagcgg tcgcgcacaa
     5581 tttcgacgcg atgttcggct cccgtgaaaa ccgtagtcac atcaggatcg atctggctcg
     5641 gctggcagtg aaggacgagg ccggcgctcg atttcgcaca gggatgttgg gtgagaagga
     5701 ggacgctggc gtgggtacgc tccgctgcag cccggtaccg gaaccaggtg gccaggggaa
     5761 cgcgaagtgc gaactcaggc gcaatcgaac cgaggtcgag aacgatggcg ctgaagcctc
     5821 cgccctgcag gagcagatcc gtggcacgga gtgcctgatc cagcctcgac cagggctttt
     5881 tgcgagaacg ctgaggcgct aagttgggag tttggagttg agagtttggc tgacctgggc
     5941 tcaggttgcg agttacaggt tgcggcacca cttccctgcc atgacgtcgg acttttggca
     6001 ccggttcgca gcagcggggt gccaggtaat cgtggcgaat cggtgggctg tctctatcgg
     6061 tgaaagaggg ttgcgatttt gcggtagcta aatcgttgcg aacggccagt cgttgctttt
     6121 cgaaaaacat gtccgattgt ttgtcggaca tgcctggcaa agtaagctgt tctggctcag
     6181 tgccgcagcg aatccagagc aatcggttga ggtcgatgcc ggcaccagcg gcagactccg
     6241 ggtggagcgc gtccgacacg tcgacccagg cgcagacgcg gttgtcgttt gtcagacacg
     6301 cgagaaaagt gagggccagc gatgtccggc ccgagcactc cggaccggtg agttcggtga
     6361 tggcgccgat gggaaggccg ccctgaagag cgtcatcgag ggcggtaagg cccgtaggcg
     6421 cattcggaca caggacacgc ggaggtggtg tgagcgccga ggggatacgg tcggcgagaa
     6481 cagcttcaat ctgttggcgg agggcggcgg cggttggcat acggcaacac tttcgcctta
     6541 tcttcgccta ttggatgcgg gggagcaagg ggcggatttg cacagaagtg gctaaataag
     6601 cggatggctt ggaagcaaag agcttattcg atggcaaaaa ccgcatagac cgtcgccgat
     6661 tcttcaattt gttgcggacg gatagcgagg ggcgagggtg gcggagccgc ttttgcggca
     6721 gcacccatga agtcgtagac tctgggcggt gtcggctgtg ccggcgcctg attgctggcg
     6781 tagaccagcg ccccaagact cactcccagt cccgaagcca tttgcgcagc gatggcgtgc
     6841 gcatcaacaa gtgccttctg cgccgcctgc gcctgcaatc ctttgcgatc ggtcacgtcc
     6901 caggcgatat ttccgctctg attggcgccc gcctcgattg ccacatggag caccatcgcg
     6961 gcgtctctcg cggcggtgtg cacggcccag ctctgcgaaa gtgtgaatgc cttctgaatc
     7021 cgttcctcgg gtgccggttg gggattgtat ggaaactcag ttttgccgag gttctgctcc
     7081 tggctttcga tcgccttgtc cgccacgccg gccttcttca gcgcagcaag gatcgcgttc
     7141 gacaccgctg atcccttgct gtaggcagcc gccgcatcgg gcgcataaat ctgaaaaccc
     7201 accgacacca cggccgtgtc tgcctccgcc gaggccttgt cgctggccgt gaccgcgatg
     7261 gtgcggttgt tcttgtccac ctggatggtc tgcgcgctgg ctacaacggc gccgaacagg
     7321 acggccagag ccagggaaag agtccgtttc atcatcacaa ccctcacgag ggattgtacc
     7381 tggcacaagg tcggagcctc gccgtgcgct atgctaggct caagtttcgg agcggagtgc
     7441 ccttgttcgg catccaaacc ggttacggat ctacttcacg tatttctata taacgaatgg
     7501 ctctggagac catcgacgct gccgccaaac gaggcgttct cgccctgcag gactacgcgc
     7561 tcctctcggc tcgtgcgctt ggggatattt ttcggcgtcc gttctaccgt tccgacattt
     7621 tcatgcaggc cgatatcatc ggggtcggtt cgctgcccat cgtgatcctt acgggattct
     7681 tcctgggcgg cgtattggcg ctgcagtccg cggcgacgct gtcgcagttc ggtgctgcgg
     7741 cgttcaccgg gcagctggtg gcgttttcga tgatcaagga gatcgggccg gtggtgacag
     7801 cactcatggt ttccgggcgc aacgcgtcag gaatggccag cgagctgggt tcgatgatcg
     7861 tgaccgaaca aattgacgcc atgcgtgcgc tgggcaccga tccgctgaag aagctggtca
     7921 tgccgcgcat cgtcgcaacc gtggtgatgt tgttcttcct cgtcatcatt tctgacgctg
     7981 taggcatctg cggcggcggg ttcgtttccg tgttcatgct cggacaggac ggctcgcagt
     8041 acttccatac gtcgtatcag tcgctggagt atcccgacat tctgcagggg ctggtgaagc
     8101 cgattttctt cgggttcatc atcgcaacca tcggctgttt ttacggcatg aagacgcgcg
     8161 gcggaaccca gggcgtcggc cgatcgacga cgcaggccgt ggtctggtcg tcgattctca
     8221 tcatcgcctg cgacttcatg atcagccgcg tcatgatcgg catctttggg cggtgaaccc
     8281 agtggccggt gaagcaggcg gcaacggagc gacttcagcg gctgcggccg gcactcccgg
     8341 agaaccggtt gtcgttttcg aaaacgtctc catcggattc gaacgcaagc cagtgctcga
     8401 ggatatttcg ttcgaggtac gctcgggcga gatgcggatt ctgctggggc cggccgg
//
LOCUS       DASZYN010000228         5433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_199862, whole genome shotgun sequence.
ACCESSION   DASZYN010000228 DASZYN010000000
VERSION     DASZYN010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199862"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1089)
                     /gene="trpD"
                     /locus_tag="VHE33_05885"
     CDS             complement(13..1089)
                     /gene="trpD"
                     /locus_tag="VHE33_05885"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HVT97017.1"
                     /translation="MPALQALLKPIIEERRTLTRDEARETLHAILSGESSAGTDLEIA
                     ALLTAIASRGETVDELTGFAQAMRALSLPIPLEPEERAALVDTCGTGGDGVGTFNIST
                     GAALVATAAGAKVAKHGNRGITSRCGSADVLEAMGVPVALTPEQSAACLRATGFTFLY
                     APALHPAMKRVQPIRRALGFRTIFNLAGPLTNPAEAPAQIMGVYSPAKLDVAAGAMAR
                     LGVRFGRVVHARNGIDEIALSPTDTIVVRALSDHGSPHAISAHVSPEDAGLKPAKLSD
                     LQGGDIPQSNAAILQSILSGEERGPKRDVVLLNAAAALEAVGLAADLRGGVARAAEAI
                     DSGAAGNTLRALREFGRSLGPHNL"
     gene            complement(1830..2594)
                     /gene="aqpZ"
                     /locus_tag="VHE33_05890"
     CDS             complement(1830..2594)
                     /gene="aqpZ"
                     /locus_tag="VHE33_05890"
                     /inference="COORDINATES: protein motif:HMM:NF003838.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HVT97018.1"
                     /translation="MNLSKKLFAEFFGTAWLVFGGCGAAVLAAAFPGLGIGFVGVALA
                     FGLTVLTMCYAIGHISGCHLNPAVSVGLAVGRRFPWAHLVPYIVAQVAGGFVGAATLY
                     VIARGKPGFVLGSFACNGYAAHSPGLYSMQACFVAEMVLTFFFLFIIMGSTDIRAPKG
                     FAPIAIGLGLTLIHLIGIPVTNLSVNPARSTGPAIFVGGWALSQLWLFWLAPALGGVL
                     GGLVYRVFFAEKPAPGVQAVPAKPAYEPLPGTSAGD"
     gene            2890..4068
                     /gene="sucC"
                     /locus_tag="VHE33_05895"
     CDS             2890..4068
                     /gene="sucC"
                     /locus_tag="VHE33_05895"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HVT97019.1"
                     /translation="MKIHEYQAKDILAKYGVAIPRGEVANSVDEAIEAARALFSGGAK
                     GVVVKAQIHAGGRGKGGGVKVAKTLDEAEQYAKQILGMQLVTHQTGPEGQKVQRLLIE
                     ETAAIDRELYLGIVLDRAAAKLVFMASQAGGMEIEEVAAKDPKAIYKAYIEPGVGFQP
                     YQARELAFALGLKPAQIADAVKFMTGIYRAYIDTDASLLEINPFITTKDDKLFALDCK
                     INFDDNAMFRHKDLKELRDVAEEDPLEVEASKYALNYIKLDGNIACMVNGAGLAMATM
                     DIIQYAGGMPANFLDVGGGANQQQIEHAFEILLSDKNVQAVFINIFGGILRVDTLATG
                     VVEAAKNLKVKVPVVLRLEGTNVEEGRKILKDSGLKFIVGDTMQDAAQKVVAAAKGAA
                     "
     gene            4071..4943
                     /gene="sucD"
                     /locus_tag="VHE33_05900"
     CDS             4071..4943
                     /gene="sucD"
                     /locus_tag="VHE33_05900"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF004230.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HVT97020.1"
                     /translation="MAVLVDKNTRLIVQGITGREGTFHAKGCAEYGTKVVGGVTPGKG
                     GTTHEGWPVFDTVHDAVEKTGANVTVIFVPPPFAADAILEANDAGLPLIICITEGIPI
                     NDMVKVWSVLKHSNSRLIGPNCPGVISPGKAKVGIMPGRIHKEGSIGIVSRSGTLTYE
                     AVYQLTQRGIGQSTAIGIGGDPIIGTTHVDALKLLNEDPETEAIILIGEIGGTAEETA
                     AAYVKEHVKKPVVGFIAGQTAPPGRRMGHAGAIISGGQGTAAEKMKALTAAGIHVVET
                     PAAIGETVAKVIGK"
     gene            5027..>5433
                     /gene="ndk"
                     /locus_tag="VHE33_05905"
     CDS             5027..>5433
                     /gene="ndk"
                     /locus_tag="VHE33_05905"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HVT97021.1"
                     /translation="MSQRTFSIIKPDAIRKGHAGAILAEIEKAGFKIVAIKKLSISKP
                     QAEGFYYVHKERPFFGSLTTFMSSGPIVALVLEKENAIADLRKLMGATNPANAEEGTI
                     RKKYAGSIEENAIHGSDAEETAQFEIGYFFAGH"
BASE COUNT         1096 a         1284 c         2051 g         1002 t
ORIGIN      
        1 cggggaagcg aatcagagat tatgggggcc aagactgcgg ccgaattcgc ggagagctcg
       61 gagggtgttg ccagcggcac cggagtcgat ggcttcggcg gcgcgagcaa cgccgccgcg
      121 gaggtcggca gcgaggccga cggcttcgag cgcagcggcg gcgttgagga ggacgacgtc
      181 gcgcttcggg cctctctctt cgccgctgag gatggattgg aggatcgcgg cgttgctttg
      241 cggaatatcg ccgccctgga gatcagagag ctttgcgggt ttcaggccgg cgtcttcggg
      301 agagacgtgg gcggatatcg cgtggggtga accgtgatcg gaaagagcgc gaacgacgat
      361 ggtgtcggtg ggagagaggg cgatttcgtc gatgccattg cgggcgtgga caacacgacc
      421 gaaacggacg ccgagacgag ccatggcgcc ggcggcaacg tcgagcttcg cgggagagta
      481 aacgcccatg atttgtgctg gagcctcggc cggattcgta agggggccag cgagattgaa
      541 gatggtgcga aaaccgagag cgcggcggat cggctggacg cgcttcatgg ccgggtggag
      601 ggcgggcgcg tagagaaagg tgaatcctgt agcgcggagg caggcggctg actgctccgg
      661 cgtgagggcg acgggaacgc ccatggcttc gaggacgtct gctgagccac atctggaggt
      721 gatgccgcgg ttgccgtgct tggcgacttt ggcgccggca gcggtggcca cgagggccgc
      781 gccggtagaa atgttgaagg tgcccacacc gtcgccgccg gttccgcagg tatcgacgag
      841 ggcggcgcgt tcttcaggct cgagcggaat ggggagagac agggcgcgca tggcctgggc
      901 aaaacctgta agctcgtcga cggtttcgcc gcgactggcg atggcggtga gcaatgcggc
      961 gatttcgagg tccgtgcctg cgctggactc tccactcaga atggcatgga gcgtctcacg
     1021 ggcctcatcc cgcgtgaggg tgcggcgttc ctcgatgatc ggtttgagga gggcctggag
     1081 agcgggcatg tgaagccatg ctaacgcgac ggcccaggcg ggccgaaatg cggaaggggg
     1141 aggccggctg ggttctgcgg cgaaatagta tccgcaagga ggggctttca gggagtttgg
     1201 gttgctggcg ggtctatcgg ttcctcagca tagcaactcg ttcgtgggtg cagggccgtt
     1261 tcccgaagtg gttttgtgac cggtactttt gggtcaggct cccgcaagtg ttcgttgacg
     1321 aggccgtccg gagggcgtct tctgcttgct gtggggtgaa cctggcgtct tttcttcggg
     1381 gagttggggg gccagggtcg gtgttcccgt ttcgggtctg agtttccgat tcgggccgtg
     1441 agcggtactt gggttggaat ctttgctcga ttggattgac cagagccacg cggagggcgt
     1501 cttctgcttg ctgtggggtg aacctggcgt cttttcttcg gggagttggg accagggtcg
     1561 gtgttcccgt ttcgggtctg agtttccgat tcgggccgtg agcggtactt tgggctggaa
     1621 tcttcgctcg atttgattga ccggagccac gcagagggcg tcttctactt gccgtggggt
     1681 gaacctggcg tcttttcttc ggggagttgg cctttttcgc ttgtttggaa ggcttttcgt
     1741 gtgttggaac gcttttcggt tgtttggaag ccctgcgaga ttggaggcgg ctgcgcgctg
     1801 aggcagccgc cttcggtggg acacgtcgat tagtcgccgg ctgaggtgcc gggaaggggc
     1861 tcgtacgccg gtttggcggg gacggcctgg acgccgggtg ctggtttttc agcgaagaag
     1921 acgcggtaga cgaggcctcc gaggacgcca ccgagcgccg gggcgagcca gaagagccag
     1981 agttgggaaa gggcccaccc accgacgaag atcgcggggc cggtgctgcg agccgggttg
     2041 acggagagat tggtgacagg gatcccgatg aggtggatga gagtgaggcc gagcccgatg
     2101 gcaatggggg cgaagccttt gggagcgcgg atgtcggtcg atcccatgat gatgaagagg
     2161 aagaaaaagg taaggaccat ttcagccaca aagcaggcct gcatgctgta gaggccgggg
     2221 gaatgggctg cgtagccgtt gcatgcgaac gacccgagga cgaagccggg cttcccgcgg
     2281 gcaatgacat agagagtggc ggcgccaacg aatccgccag cgacctgcgc gacgatgtag
     2341 ggcacgaggt gcgcccacgg gaagcggcgg ccgacggcga gaccgacgga gacggctgga
     2401 ttgaggtggc agccagagat gtgtccgatg gcatagcaca tcgtgagcac agtgaggccg
     2461 aaggcaagag ctacaccgac aaagccgatg ccgagaccag gaaaggcagc ggcgaggacg
     2521 gcggctccgc atccgccaaa gacgagccag gcggtgccga agaattcggc gaagagtttt
     2581 ttgctgagat tcatttttcc ctcctgttga aaccgagtca tttgcggctg accgaatcga
     2641 aaagcaacga agagcccgac gagaaaggtg gcgcgggaga gacgagaacg ggagaggcgg
     2701 acagtgtagc gcagatggag aggcctgggg agtgaaggag aaggatttgc ggaggagcgg
     2761 cggaggtttg cagcaggctg gatgcgttcg ctacactgag aattctgctt tagccgcggc
     2821 gggcgcgagc gcgcaacggc gagacctcac gaagatccct gcaccatcaa agctcctctc
     2881 aaatcattta tgaaaattca cgaatatcag gcaaaggaca ttctggcgaa gtatggcgtc
     2941 gcgattccgc gcggcgaggt cgccaacagc gtggatgaag ccatcgaggc ggcgcgggcg
     3001 ctgttcagcg gcggcgcaaa gggtgtggtg gtgaaggcgc agatccacgc cggcgggcgc
     3061 ggcaagggcg gcggcgtgaa ggtagcgaag actctggatg aagccgagca gtacgcgaag
     3121 cagattcttg gcatgcagct ggtaacgcac cagacgggcc ccgagggaca gaaggtgcag
     3181 cggctgctga tcgaggagac agcggcgatc gaccgcgagc tgtatctggg gatcgtgctg
     3241 gatcgcgcgg cggcaaagct ggtgttcatg gcgtcgcagg ccggcggcat ggagattgaa
     3301 gaggtcgcgg cgaaggatcc gaaggcgatc tacaaggcgt acatcgagcc gggagtgggg
     3361 ttccagccgt atcaggcgcg cgaactggcg ttcgctctgg ggctgaagcc agcgcagatt
     3421 gcggacgcgg tgaagttcat gaccgggatt taccgggcgt atatcgacac ggacgcttcg
     3481 ctgctggaga tcaatccgtt catcacgacg aaagacgaca agctgtttgc gctggactgc
     3541 aagatcaact tcgacgataa cgccatgttc cggcacaagg atctgaagga gctgcgcgat
     3601 gtggcagagg aggatccgct ggaggtggag gcgtcgaagt atgcgctgaa ctacatcaag
     3661 ctggacggca acatcgcgtg catggtgaac ggagcggggc tggcaatggc aacgatggac
     3721 attatccagt atgcgggcgg gatgccggcg aattttctgg atgtgggcgg gggcgcgaac
     3781 cagcagcaga tcgagcatgc gttcgagatt ctgctgtcgg acaagaacgt gcaggcggtg
     3841 ttcatcaata tttttggcgg gattctgaga gtggacacgc tggccacggg agtggttgag
     3901 gcggcgaaga atttgaaagt gaaggtcccg gtcgtgcttc ggctggaagg cacaaatgtg
     3961 gaagaggggc gaaagatcct gaaggattcg ggattgaagt tcattgtcgg agacacgatg
     4021 caggatgcgg cgcagaaagt ggtggcagct gcgaaaggag cggcgtagag atggctgtac
     4081 tggtcgataa gaacactcgc ctcatcgtgc aggggattac cggacgcgaa ggaactttcc
     4141 acgccaaagg ttgcgcagaa tacggaacga aggtggttgg gggcgttacg ccgggcaagg
     4201 gcgggacgac acacgagggg tggccggttt tcgatacggt gcatgacgcc gtggagaaga
     4261 caggcgccaa cgttacggtg atttttgtgc cgccgccgtt tgctgccgat gcgattctcg
     4321 aagcgaacga tgctggtttg cctctgatca tctgcatcac cgaggggatt ccgatcaatg
     4381 acatggtgaa ggtttggagc gtcctgaagc attccaactc acggctgatc gggccgaatt
     4441 gcccgggcgt gatttcgccg gggaaggcga aggtaggcat catgccgggg cggattcaca
     4501 aggaaggctc gatcgggatc gtgtcgcgct cgggcacgct gacctatgag gcggtgtatc
     4561 agctgacgca gcgcgggatt gggcagtcga cggcgattgg gattggcgga gatccgatta
     4621 tcggcacgac acacgtggac gcgctgaagc tgctgaatga ggatccggag accgaggcaa
     4681 tcattctgat cggcgaaatt ggcggtacgg cggaagagac cgcggccgcg tatgtgaagg
     4741 agcatgtgaa gaagccggtg gtgggattca tcgccggaca gacggctccg ccgggacgac
     4801 ggatggggca tgcgggcgcg atcatctcgg gcggccaggg cacggcggcg gagaagatga
     4861 aggcgctgac ggcggcgggg attcatgtgg tcgagacgcc ggcggcgatt ggggagacgg
     4921 tggcgaaggt gatcgggaag taagttgggg cagttgttag ttcttagttg tgagtcgcaa
     4981 gttctaattt gaaagccgtt tcttgagtta gaaggccagg agacctatgt cacagcgtac
     5041 gttcagcatt attaagccgg atgcgattcg gaagggtcat gcgggggcga ttctcgcgga
     5101 gattgagaaa gccgggttca agattgtcgc gatcaagaag ttgtcgattt cgaagccaca
     5161 ggccgagggc ttctactacg tgcacaaaga gcggcccttc tttggatcac tgacgacgtt
     5221 catgtcttcg gggccgattg tggcgctggt gctggagaaa gagaacgcga ttgccgatct
     5281 gcggaagctg atgggggcaa cgaatcctgc gaatgcagag gaggggacga tccggaagaa
     5341 gtatgcgggg tcgatcgagg agaatgcgat tcacgggtcg gacgccgagg agacggcgca
     5401 gttcgagatt gggtatttct tcgccgggca tga
//
LOCUS       DASZYN010000229         7363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_391497, whole genome shotgun sequence.
ACCESSION   DASZYN010000229 DASZYN010000000
VERSION     DASZYN010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7363
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391497"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4010)
                     /locus_tag="VHE33_05910"
     CDS             complement(<1..4010)
                     /locus_tag="VHE33_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HVT97022.1"
                     /translation="MSSLVEFLSDLRAGGIILSAEGDRLLCNAPRGAMTEDIRKTIAA
                     HKPEILDFLRSSAGTITARQGKPGDASPLSRSQQRLWFVSQMDPGNPVYNVVIALGLH
                     GPLHQSAMEQSLRALVDRHESLRTSFHQQEGKPWSEVSERVEWRCSFVDLSAHPPSFA
                     AEEARRLALEESRRGFDLSSAPLFRASLFRISPDEHLLLLVVHHIVADGWSLGLIAKD
                     LGAFYAAVVAGKNPNLAELPATYRDYVQWEQQDVVRATREQLPFWTEQLKGAPPILEI
                     PGDRQRPALQTFRGQRIAVRIEPELAQRIADLCRTTGSTSFMVLLAGFTVLLHQYTDQ
                     DDILVGSATSNRQRKEFAPIVGFFVNNLVLRTHLGGNPSFSELLQRVRKTAHDAYAHQ
                     EIPFDLLVEKLQPQRRTGHSPLVQVFFTLQNLPLEAIELAGLRVVPVQLDPGIARADL
                     AVEIWPEADGYRCDFEYSTDLYNDETMTAMQAQYVRLLREAVERPDTPIRQLPLLSDE
                     ESRQLLVRRRRTASPVPNSLNGSPIETARAVELTRRASSGSGAMRADILPGEHPLSRS
                     QQRLWFLDQLDPGNPVYNIAIAIRLDGPLNRAAFEDALRFILRRHESLRTRFIERDGV
                     PVAVIDEGEDWRLDFVDVSAVPEREQHETILGLVLKESQRPYVLDQGPVFRVTLYRKN
                     DQEHVALIGMHHIVSDGWSLGVLSQELGRVYQAYVRHETPPLPPLPLQFREFVQWEHD
                     QQLQSSADLEYWRSKLSGELPQLALPLDHPRPPVQTFRGQRATTEIPADLENQLLALA
                     RRHNASFYMVLLAAFGVMLRQFTRQDDILIGTPTAGRLKSSFESLIGFFVNNLVLRTD
                     LSGDPSFADLVGRVRTLALEAFEHQSTPFDQLVELLQPERTLDRSPLFQVMFTLQNQD
                     ASLPPMGDIAISQLDFQGFRARYDLAVDVYPHEGRFRCDFEYNTDLFEEATMQRMLRQ
                     YLHILELACADATQPIHKLQLLAEPERRQLIEGWNQTVVPPAPWATVPAWFAAQARET
                     PASIAVSMGARSLTYSELDAQSSALASALRAQGISRSDVVAVFLERSPEMVIAVLGIL
                     KSGAAYLPLDPALPAQRIEFLLGDANVPLILTQQELRSTLPPTNATVFSIDELDLSPA
                     DAPSIQLSGDDLAYLIYTSGSTGNPKGTEIPHSALVNLLASMLREPGLSATDTLVSVT
                     TLSFDIATLEVFGPLLCGAKLVIASRDQVIDPEQLAELLDVSDATVMQATPSTWRMLI
                     ESGWMGRPRLRMWCGGEALAPDVAEGLMARGSELWNLYGPTETTIWSAAHRVRSGENP
                     ILIGRPIANTQMYILD"
     gene            complement(4007..>7363)
                     /locus_tag="VHE33_05915"
     CDS             complement(4007..>7363)
                     /locus_tag="VHE33_05915"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT97023.1"
                     /translation="LRETLAERGIAAQALTVSHAFHSPLMEPMLDEFEQIAQGIQHHA
                     PQVDLVSNVTGTCLGPSDRITADYWRRHVRGTVRFADGVASLMNRKPAALLEIGPDPV
                     LLGMAKPALPSAVPTLSSMRRGKDLWSGLYESLRQLYLLGAPVAWDEVYRDTTAQKLE
                     LPTYPFERQRYWISPASKKTKSAPSEAQSFDPLLFTTRWQPLATGGASARPDELVDVA
                     ELEFLTDNTVSDQYATFLPQLNHLCALYILDTLRHVGVRVASGELLPIAALAERFPSH
                     RRLLARMGSILREDGIVEQAANGLLFRSIPEEIATAEHARLVAQWPAFSAELGFLAQT
                     SRMHEVLTGTASALEVLFPAGSLQLAERVYQDAPAAQAFHRATAVAVREAVQSYAAGQ
                     KVRVLEVGAGTGSTTSYVLPLLANLAVDYRFTDISPAFLEKARAKFSAFPFVRYATLD
                     LENQSQLTAAEPADVVIAANVVHATADLRQTLARLRQTLAPGGVMILVEGTAPQRFGD
                     LTVGMTEGWWRFTDTGLRPDYPLISREQWLGLLREAGFEADALGVSSTLGSLATQQTI
                     LIAQLPQARSFAPLRSTPLRLQAGQAHNKARIQEHLAGASTNPRIVVESAHPAADVPQ
                     ATFNFACEALGTLQQVAEQASGAAEIWFVVHDSPDGLGALLFSATEALAKTAQIEHPQ
                     LRVRCVRLPQDPTPHDLSQFEELLLQETSELSFRVQNGTISVQRLVRPEEAGATSWSA
                     PRLRADAAYLITGGYGGLGFRTAEWMVDRGAKHIFLTGRRPPSAETQQALDLLHARGA
                     KVVALTGDISEHDHVQAIFAQIQASGSPLRGVIHAAGTVEDSTLLQQSPAHLAKVFAA
                     KVNGSWLLHEATRSMALDFFVLYGSAASTLGSAGQANHSASNGFLDGLAEFRQKIGLP
                     GTTIAWGAWSEIGAATQITDTARGSRLGLGTFPPEKGIEMLEQAIGSGLSRLSALPVD
                     WPRYLVQPQGSWPLFDVQRVASGAPADVPKSSAPDLKIILADTPSDRRVEAISAAIRS
                     RAALVLRLESAAAVRDEQPFPELGLDSLMAVELKNDLQASSGVSLPANFFFEFPTVRQ
                     AAAYLNAKMAAQEDSGRRMPDSAEYEEVEL"
BASE COUNT         1572 a         2033 c         2403 g         1355 t
ORIGIN      
        1 tcgtccagga tgtacatctg cgtattggcg atgggacgtc cgatgaggat gggattctcg
       61 ccactgcgaa cacgatgggc agcggaccag atggtggtct ctgtaggtcc gtacagattc
      121 cagagttcgc tgccgcgtgc cataaggccc tcggcgacat caggggcaag cgcttcgcct
      181 ccgcaccaca tgcgcaggcg gggacggccc atccagccgg actcaatgag catgcgccag
      241 gttgatggcg tcgcctgcat gacggtggcg tcggagacat cgaggagttc agccagttgt
      301 tccgggtcga tgacctgatc gcgggaggca atcaccagct ttgctccgca gagaagcggt
      361 ccgaagactt ccagagtggc gatgtcgaag gagagagtgg tgacggagac cagggtgtct
      421 gtcgcagaaa gtcccggctc gcgcaacatc gaggcgagaa gattgacgag ggccgaatga
      481 ggaatctccg tgcctttggg gttgccggtc gatcccgagg tgtaaatcag atacgccagg
      541 tcgtcgccgg agagttggat tgacggggcg tccgcagggg aaagatcaag ctcgtcgatg
      601 ctgaatacgg tggcgtttgt gggcggcagt gtgctgcgca actcttgttg ggtgaggatg
      661 agcgggacgt ttgcgtcgcc gagcagaaac tcgatgcgct gggcgggcaa ggccggatca
      721 aggggcaggt aggctgctcc ggatttaaga atgccgagga cggcaatgac catctccgga
      781 ctacgctcaa ggaagacggc aacgacatcg gaacggctaa tcccctgcgc gcgaagagcc
      841 gatgcaaggg cggaggactg tgcgtccaac tcgctgtagg tcagcgagcg agcgcccatg
      901 gagacagcaa tcgatgccgg agtttctctc gcctgagcgg caaaccatgc cggtacggtt
      961 gcccagggtg ctggcggaac tacggtctga ttccatcctt caatcagctg gcgacgttct
     1021 ggttcggcga gaagctggag cttgtgaatc ggttgcgtcg catcggcaca ggccagttcc
     1081 agaatgtgca ggtactggcg cagcattctc tgcatggtcg cttcttcgaa aaggtccgtg
     1141 ttgtattcga aatcgcagcg gaagcggcct tcgtgaggat agacatcgac agcaaggtca
     1201 tagcgagcgc gaaaaccctg aaaatccagc tgagatatcg caatgtctcc cataggaggg
     1261 agagaggcgt cctgattctg aagcgtgaac atgacctgaa acagaggact gcgatcgagg
     1321 gtccgctcag gctgcaagag ttctaccagt tgatcgaagg gagtgctttg atgctcaaac
     1381 gcttccagag caagcgtacg aacgcggccg accaggtcgg cgaaggaagg atcgccgctc
     1441 agatcggttc gaagaacaag attgttgacg aagaaaccaa tcagggactc gaaactgctc
     1501 ttcagacggc ccgcggtggg cgtgccgatg agaatgtcat cctggcgggt aaattgacgc
     1561 agcatgacac cgaaggcggc aagcaacacc atatagaagc tggcgttgtg gcggcgggcg
     1621 agagccagca gctggttctc gagatcggcg ggaatttcgg ttgtggcgcg ctggccgcgg
     1681 aaggtctgca ccggcggtcg aggatgatcg agcggcagcg ccagttgcgg cagttcgcca
     1741 ctgagcttcg atcgccaata ctcgaggtcg gcggaagact gcagttgctg gtcgtgctcc
     1801 cactgcacga actcgcgaaa ttgcaggggg agaggcggaa gtggcggagt ctcatgacgg
     1861 acatacgcct ggtagacccg gcccagttcc tgtgaaagga cgccgagtga ccagccatcg
     1921 gagacgatgt gatgcatccc gataagcgca acgtgctcct gatcgttttt cctgtagagc
     1981 gtaacccgga acaccggacc ctggtcgaga acataggggc gctgggattc cttcaaaacg
     2041 agcccgagga tggtttcgtg ctgctcgcgc tcggggacgg cggaaacatc gacgaagtcg
     2101 agcctccagt cctcgccttc gtcgatgact gcgactggca cgccgtcgcg ttcgatgaag
     2161 cgggtgcgaa gggattcgtg ccgacggagg atgaagcgga gcgcatcctc gaatgcggcg
     2221 cggttcagag gaccatccag ccggatggcg atcgcgatgt tgtaaacggg atttccggga
     2281 tcaagctggt cgagaaacca gagtcgctgc tgggaacgag aaagtgggtg ttccccaggc
     2341 aatatgtcag cacgcatcgc cccactgccg gaggaagccc gtcgcgtcaa ttcgacggca
     2401 cgggcggtct cgatgggcga gccattgagc gaatttggaa cggggctggc cgttcgcctg
     2461 cgcctgacca ggagctgacg gctttcttca tcggagagaa gaggcagctg ccgtatcggg
     2521 gtatcgggac gctcgacagc ctcgcgcaaa aggcgaacat actgcgcctg catggccgtc
     2581 atggtctcgt cgttgtacag atcggtgctg tactcaaaat cgcagcgata gccatcggcc
     2641 tccggccaga tctctaccgc aagatccgcg cgcgcgattc ctggatccag ttgcacgggg
     2701 accacacgca agccagcgag ttcgatcgcc tccaggggaa ggttctgcag ggtgaagaaa
     2761 acctgcacaa ggggactgtg accagtacgc cgctgcggtt gcagcttctc gaccagcaga
     2821 tcgaagggaa tctcctggtg cgcataagca tcatgggcgg ttttccggac gcgctgcaac
     2881 aattcggaaa aggaaggatt gccgccgaga tgggtgcgga gaacgagatt gttgacgaag
     2941 aagccgacga tcggagcgaa ctcttttcgc tggcggttgg atgtggcgct gccgaccagg
     3001 atatcgtcct gatccgtgta ttggtgcagc aaaaccgtga agcccgcaag cagcaccatg
     3061 aaggaagtgg aacctgtggt tctgcacaga tcggcaatcc gctgcgcaag ttccggttca
     3121 atccggacag cgattcgctg gccgcgaaac gtctgcagtg ccggccgctg ccgatcccct
     3181 ggaatctcga ggatgggagg cgcgcctttc agctgttcgg tccagaacgg aagctgttcg
     3241 cgcgtggcac gaaccacgtc ctgctgctcc cactgcacat aatcgcggta ggtagccggg
     3301 agttcagcca gattggggtt ctttcctgcg acaacggcag catagaacgc accaaggtct
     3361 ttggcgataa gacctaacga ccatccgtca gcgacgatgt gatgaaccac gagcagcaga
     3421 aggtgttcgt caggggagat acggaagaga cttgcacgga acagaggcgc cgaggacaga
     3481 tcgaacccgc gacgcgactc ctcgagcgca agacgacgtg cctcttcggc cgcgaaggaa
     3541 ggggggtgag ccgagagatc gacaaagctg catctccatt caactcgctc ggatacttct
     3601 gaccagggct tgccttcctg ctggtgaaac gaggtgcgca gcgactcgtg acggtcgacg
     3661 agagcacgga gcgattgctc catcgcactc tgatgcagag gaccgtggag gccaagcgca
     3721 ataaccacgt tgtagaccgg attgcccgga tccatctgag agacaaacca cagccgctgc
     3781 tgtgagcgtg agagaggaga ggcatcgcct ggtttaccct gtcgtgccgt aattgtgccg
     3841 gcagaactgc gcaggaaatc gagaatctcc ggcttgtgcg cggcgatggt tttacgtatg
     3901 tcctccgtca tggcgcctct gggcgcgttg cagagcaggc gatcgccttc tgccgagagg
     3961 atgatcccgc cggcgcgcaa atccgacagg aactcaacca gagaactcac agttcaacct
     4021 cctcatactc tgcagagtcg ggcatgcgac gaccggaatc ctcctgagcc gccatcttcg
     4081 cgttgaggta agcggcggcc tgacggaccg tcggaaattc aaagaagaaa ttcgcgggca
     4141 gggatacgcc ggaacttgcc tgcagatcat tcttcaactc cacggccatc agagagtcga
     4201 ggccgagttc gggaaaaggc tgttcgtcgc gaacggctgc cgcagattcc agccgcagaa
     4261 cgagcgcagc acggctgcga atcgcagcgg agatcgcctc gactcttcgg tccgaaggag
     4321 tatcggcaag gataattttg agatcaggag cggaggattt gggcacatca gcgggggcac
     4381 cgctcgcgac cctttgcacg tcgaacaatg gccacgaacc ctggggttgg accagataac
     4441 gcggccaatc aacgggcaaa gccgatagcc gggacaagcc gctgccgata gcctgttcga
     4501 gcatctcgat tcccttctcc ggcggaaagg tcccgaggcc aagacgcgag ccgcgcgccg
     4561 tatcggtgat ctgggttgcg gcgccaatct ccgaccaggc gccccaggcg atggtggtcc
     4621 cgggaagacc aatcttctga cggaattcgg cgaggccgtc gagaaatccg ttggaagcag
     4681 agtgattcgc ctggcctgcg gatcccaggg tcgacgccgc agaaccatag agaacaaaga
     4741 agtccagggc catcgaccgt gttgcttcgt ggagcagcca gctgccgttc acttttgcag
     4801 cgaagacctt tgcgagatgt gccggagatt gttgcagcag agtgctgtcc tcgaccgttc
     4861 ccgcggcatg aatcacgccg cgcagcggag atccactggc ctggatctgg gcgaagatcg
     4921 cctgaacgtg atcgtgctct gaaatatcgc cggttaatgc gacgactttt gcgccacggg
     4981 catgaagaag atcaagcgcc tgctgtgttt cggcagaggg cggacggcgg ccggtgagga
     5041 aaatatgctt tgcaccgcga tctaccatcc attccgcggt gcggaacccc aggcctccgt
     5101 agcctccggt gatgaggtaa gcggcatcgg cgcgaaggcg cggggcgctc cacgaggtcg
     5161 cgccagcttc ttccgggcgg acgagtcgct gcaccgagat cgttccattt tgtacccgaa
     5221 acgacaactc actggtttcc tgaagaagca gctcttcgaa ctgcgagaga tcatggggtg
     5281 tcggatcctg gggcagacgt acgcagcgaa ctcgaagctg gggatgctcg atttgcgcgg
     5341 tctttgccaa tgcttccgtc gcggaaaaga ggagcgctcc caaaccgtcg ggtgaatcgt
     5401 gcacaacgaa ccagatttcc gcggctccgg aggcctgttc ggcgacctgc tgcagtgttc
     5461 cgagcgcctc gcatgcgaag ttgaatgttg cctgggggac gtcggcagcg gggtgggcgc
     5521 tttcgacgac aattcgtggg ttcgttgaag cgcctgcgag atgctcctga attcgcgctt
     5581 tgttatgtgc ctggcctgcc tgcagacgca acggcgttga tcgcagtggg gcaaatgacc
     5641 tggcttgcgg cagctgcgcg atcaaaatgg tttgttgcgt cgcgagcgaa cccaaagtgc
     5701 tggaaacgcc cagtgcatcg gcctcgaaac ctgcctcacg cagcaatcca agccactgct
     5761 ctcgagaaat cagtggatag tccggccgga ggccggtgtc ggtaaaccgc caccatcctt
     5821 ccgtcatgcc aacggtcagg tcgccgaagc gctgcggcgc ggtgccttcg accagaatca
     5881 tcacaccgcc gggtgcaagg gtttgccgga ggcgcgcgag ggtctggcgc aggtcagccg
     5941 tagcgtgcac aacgttggcg gcgataacca cgtcggcggg ttctgctgcc gttaactgag
     6001 attggttttc gagatcgagc gtcgcgtagc gaacgaatgg aaatgcggag aatttggcgc
     6061 gggctttttc gagaaaggca ggagagatgt cggtgaagcg gtaatcgacg gcgaggtttg
     6121 cgaggagcgg caagacatac gaggtcgtac ttcccgtgcc cgcacccacc tcaagaacac
     6181 gcaccttctg tccagcggcg tagctctgca ccgcttctct cacagcgacc gcggtcgccc
     6241 gatgaaaggc ctgcgcagcg ggagcatcct gatacacgcg ctccgcgagt tggagtgaac
     6301 cggcgggaaa aagtacttcg agggctgacg ccgtccctgt cagcacttca tgcatgcggg
     6361 acgtctgcgc gagaaagccg agttcggcgg agaaagctgg ccattgggca acgagtctgg
     6421 catgttccgc tgtggcaatt tcctcgggga tcgaccggaa aagcaatccg ttcgcggcct
     6481 gttcgacgat gccatcttcg cgaagaatag agcccatcct ggccagcaga cggcggtgcg
     6541 agggaaagcg ctctgcaagc gctgcgatgg gcaggagttc ccccgatgca acgcgcactc
     6601 caacgtggcg cagagtgtcg aggatgtaaa gcgcgcagag gtgattcagc tgcggaagaa
     6661 acgtcgcata ttggtccgaa acagtgttat ccgtgaggaa ttctaattcg gcgacgtcca
     6721 ctaattcgtc ggggcgggcg gatgcgcctc ctgtagccag cggctgccac cgggtcgtaa
     6781 acaaaagagg atcgaagctt tgcgcttctg acggcgcgct ttttgtcttc ttactcgccg
     6841 gggatatcca gtagcgctgc cgttcgaacg gataagtggg aagctccagc ttttgcgccg
     6901 tggtatcgcg gtacacctcg tcccatgcaa ccggcgcgcc gagcaggtag agctgccgca
     6961 acgactcgta aagtccggac cagagatcct ttcctcgccg cattgaggaa agggttggaa
     7021 ccgccgaagg cagggcaggt tttgccatac ccagaagcac cgggtcggga ccgatctcga
     7081 gcagcgctgc cggtttgcga ttcatgagtg acgcaacacc gtcggcaaaa cgaaccgtgc
     7141 cgcgcacatg acgccgccag tagtcggcgg ttatgcgatc ggaaggtccg aggcatgtgc
     7201 cggtgacatt cgacaccaga tcgacctgtg gtgcgtggtg ctggatgccc tgcgcaatct
     7261 gctcaaactc gtccagcatg ggctccatca gcggtgagtg aaaagcgtgg gagacggtga
     7321 gagcctgagc tgcgattccc cgctctgcca gggtctcgcg cag
//
LOCUS       DASZYN010000230         3654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_9645, whole genome shotgun sequence.
ACCESSION   DASZYN010000230 DASZYN010000000
VERSION     DASZYN010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9645"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VHE33_05920"
     CDS             <1..182
                     /locus_tag="VHE33_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97024.1"
                     /translation="PLSWDSTAAPVAPNGNSIAQKRRQERKNFLLLPVDFTEQYKQIV
                     VVSSWPPNCRGIAQE"
     gene            249..602
                     /locus_tag="VHE33_05925"
     CDS             249..602
                     /locus_tag="VHE33_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97025.1"
                     /translation="MDKLSAKIENWAVVDNLIFHGFRKLEPGQRLTGYLMGHSDLPNG
                     VIYTSVIQRVDQANGLVETGNTVYRLGQVNEFYQQWATNEAEEATQQVHFMPDSRRPS
                     AGYPAVGSIPAYRQG"
     gene            complement(667..2670)
                     /locus_tag="VHE33_05930"
     CDS             complement(667..2670)
                     /locus_tag="VHE33_05930"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HVT97026.1"
                     /translation="MNLDDLARFHDVMGPVVSPDGQWVAYTVSTIEAKADKRITDLWM
                     VSWDGKEDVRLTWAGDVDEESDDLSSSGAPRWSPDGKFISFSADRKGPAKGAQVWVID
                     RRGGEAHQLTDVKGHLSAYAWAPDAKKLLLSISPDEEAEAAAKKPEGEKEKEKPKPIV
                     LDRYHFKQDVEGYLKSNEHTQLFLWDVATKKLEKLTTDKDYDEGNAVWSPDGTKIAFV
                     SNHDPDPDRSINSDVWVVDAKPNSTPKRLTTYDGPDDGRIAWSPDSKWIAFMEGDPLK
                     LWQYSEEKLGIVAADGSAAPRILTTALDRGVSSPLFSPDGKSIEVVVEDDRDEYPASV
                     DIATGRVHRDVTEAGATFGMNQVAGHTAVVWSTNTQPGELYALENGSLRKLTHHNDDE
                     VASLKLAETRDVKATEKDGTEVHGLLTMPLDWKEGTKAPLLLFIHGGPNGQDNHAFNL
                     HRQLFAAHGYAELNVNYRGSAGRGNAYQKVIAADWGDHEVADLQAMVDEVVKEGFVDP
                     DRMGVGGWSYGGILTDYTIASTTRFKAASSGAGMGDPLVLYGVDEYILQYDNELGPPW
                     KDPQKYIQLGYPFFHADRIRTPTLFMGGDKDFNVPLNGGEQMYQALKSLNVPAELIVY
                     PGQFHGFTRPSFIRDRYQRWFDWYDKWVLGKTPAAPAGTEVKK"
     gene            3062..3343
                     /locus_tag="VHE33_05935"
     CDS             3062..3343
                     /locus_tag="VHE33_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97027.1"
                     /translation="MNKSDKPIVRIENWSVVGSVIYYGFRRLELGARLTGDVMGHANL
                     RNGTIYTSAILSIDRLKNHVETHNSIYQLGEVSDEYARWIEAQEKPQAA"
     gene            complement(3470..>3654)
                     /locus_tag="VHE33_05940"
     CDS             complement(3470..>3654)
                     /locus_tag="VHE33_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97028.1"
                     /translation="EGDVSITRMKHRIAGAEAQNAAAREMMDGDGVEAKFAKLEREEQ
                     IDALLRDLKEQHAKTA"
BASE COUNT          712 a         1207 c          981 g          754 t
ORIGIN      
        1 ttccactttc gtgggattca accgctgccc ccgtggcgcc gaatggcaac tcgattgcgc
       61 agaaacgacg acaggaacgt aaaaatttcc tcctgctgcc cgtggatttc accgaacaat
      121 ataagcaaat tgttgtagtt agcagttggc ccccaaattg ccgaggtatt gcccaagagt
      181 gaaatagtcg gcaggccgct ccccaatgca gtcgcgaccc tccggcggaa agatcaggcc
      241 cctgagagat ggataagctt tcggcaaaaa tcgagaactg ggcggtcgtg gataatctga
      301 tcttccatgg tttccgcaag ttagagccgg gccagcgcct gaccggctac ctgatgggcc
      361 actccgacct tcctaatggc gtgatttaca cgtcggtgat tcagcgcgtc gatcaggcca
      421 acggccttgt cgagacggga aacaccgtct atcggctcgg ccaggtgaac gagttctacc
      481 aacagtgggc cacgaacgag gccgaagaag cgacccagca ggtgcacttc atgcccgatt
      541 cccgccgtcc ttcggccggc taccccgccg tcggttctat ccccgcttac cggcagggct
      601 gaagcgcaga cctcacccag ccctccctcc atgcggagcg gagggctgag tcgtctccag
      661 gcgccattat ttcttcactt cggtccctgc cggcgcagca ggagtctttc cgagcaccca
      721 cttgtcatac cagtcaaacc accgctgata ccggtcgcgg ataaagctgg gccgcgtgaa
      781 gccgtggaac tgccccggat acacaatcag ctccgccggg acattcaggc tcttgagcgc
      841 ctgatacatc tgctcaccgc cattcaacgg cacattgaag tctttgtctc cgcccatgaa
      901 cagggtcggc gttctgattc gatccgcatg gaagaacggg taacccagct gaatgtactt
      961 ctgcggatct ttccagggcg gtcctaattc gttgtcgtac tgcaggatgt attcgtcgac
     1021 gccatacagc acgaggggat cgcccatacc cgcgccgctg ctcgcagcct tgaagcgtgt
     1081 cgtgctcgca atggtgtagt cggtcaggat gccgccgtag ctccatccgc caacgcccat
     1141 cctgtccgga tcgacgaagc cttccttcac tacctcatcc accatcgcct gcagatccgc
     1201 gacctcgtga tcgccccagt ccgcggcgat gaccttctga taagcgtttc ctcgacccgc
     1261 gctcccgcga tagttcacgt tcagttccgc gtaaccgtgt gccgcaaaga gctgccggtg
     1321 caggttgaag gcgtgattgt cctggccatt cggtccgccg tggatgaaca gcagcaacgg
     1381 cgccttcgtg ccttccttcc agtcgagggg catcgtcagg aggccgtgca cctcggttcc
     1441 atccttctcc gtcgccttca cgtcacgggt ctcggcgagt ttcaaactcg cgacttcgtc
     1501 atcattgtgg tgcgtcagtt tgcgcagcga gccgttctcc agcgcgtaga gctcgcccgg
     1561 ctgcgtgttc gtcgaccaca ccaccgccgt gtgaccagcg acctggttca ttccaaacgt
     1621 tgcaccagcc tcggtcacgt cccgatggac tctgcccgtt gcgatgtcca cgctcgccgg
     1681 atactcatcc cgatcgtcct cgacgaccac ctcgatcgac ttcccgtccg gcgaaaacaa
     1741 cggcgatgaa acaccacgat ccagtgccgt cgtcagaatg cgcggagctg cgctgccatc
     1801 cgcggctaca atgcccagtt tctcctcgct gtactgccac agcttcagcg gatcgccctc
     1861 cataaacgca atccacttgc tgtccggact ccaggcgatg cgcccgtcat ccgggccgtc
     1921 gtatgtggtc aggcgcttcg gcgtggaatt cggcttcgcg tccaccaccc acacgtcgct
     1981 gttaatcgac cgatccggat cgggatcgtg gttgctgacg aaggcgatct tcgttccgtc
     2041 cggagaccac accgcattgc cctcgtcata gtccttgtcg gtggtcagct tttccagctt
     2101 cttcgtcgcc acatcccaca gaaaaagctg cgtatgctcg ttgctcttta aatagccctc
     2161 gacgtcctgc ttgaagtggt accggtccag cacgatcggc ttcggcttct ccttctcctt
     2221 ctcgccctcg ggctttttcg ctgccgcctc agcctcctca tcaggagaaa tcgaaagcag
     2281 cagcttcttc gcatcgggcg cccaggcata ggcggacaaa tgccccttca cgtcggttag
     2341 ctggtgcgcc tcaccgccgc gccgatcgat gacccaaacc tgtgcgccct tcgccggacc
     2401 cttgcggtcc gcggagaacg aaatgaattt cccgtcggga ctccagcgcg gggcgccgga
     2461 tgaactcaga tcgtccgact cctcatcgac atcgccggcc cacgtcaatc ggacatcttc
     2521 tttgccgtcc caggaaacca tccacaggtc ggtgatccgt ttatcggcct tcgcttcgat
     2581 ggtcgaaacc gtgtaggcca cccactgccc gtccggcgag accaccggcc ccatcacatc
     2641 gtggaaccgc gccaggtcgt ccaaattcat gggatgaggc gcttgcgcag tggcgggaga
     2701 ttgcgcatgg gacgccgcag gtcccagcgt tatcgccccg gcgaggatca tcaaggcagg
     2761 cacacgagtg gcggagaatg cgaatgattc aggtttgcag agcatgaccg gcagtatata
     2821 gaaccaccgg cctgggaaac ggcccctgtg actaacgccc cagcgcgggc gcgagcaggg
     2881 aacaatcact ccctgactgg gaaaaaattt cacagcgcca cccgccgttg tcactaaatg
     2941 acgctattta ctcaagcgtt acgatggcac cacaattgct ttccattttc tcgaagggca
     3001 ccagccacag gcttggaccg ttgaacgcga ccggtggcgg catacgtcag gtcacgaaaa
     3061 aatgaacaag tccgacaagc cgatcgtccg cattgagaac tggtctgtcg tcggaagcgt
     3121 tatctattac ggcttccgtc gacttgagtt gggcgcccgt cttactggcg acgtgatggg
     3181 ccatgccaac ctccgcaacg gcactatcta cacgtcagcg atcctcagca tcgaccgcct
     3241 gaaaaaccac gtcgagaccc acaacagcat ctaccaactc ggcgaagtga gcgacgagta
     3301 cgcccgctgg atcgaggcac aggaaaaacc ccaggccgcc tgaagccgcg aagggcaatc
     3361 agcgaacagc ttccggcgga tctgccaggc ggagcgaacc gaagatcgct cgggaagagc
     3421 tttgccaatc gccctttgtt cacggttctg gtgcggcttc gatccgcact catgccgtct
     3481 tcgcgtgctg ctccttgagg tctcggagca gcgcatcgat ttgttcctcg cgctccagct
     3541 tcgcgaactt cgcttcgacg ccatcgccgt ccatcatctc gcgcgcggca gcattctgcg
     3601 cctcggctcc cgcaatgcga tgcttcatcc gcgtgatgct gacatcccct tcct
//
LOCUS       DASZYN010000231         4361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_199889, whole genome shotgun sequence.
ACCESSION   DASZYN010000231 DASZYN010000000
VERSION     DASZYN010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199889"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="VHE33_05945"
     CDS             complement(<1..1466)
                     /locus_tag="VHE33_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97029.1"
                     /translation="MAGAQEKGNEKAEAKPGAASTVGAPDSTTEGSVTIGGQAVAYRA
                     VAGTITVGANDQQDATLGFDGSLLPNSGVKPPADPAEAPPTARIFYVAYFRKDATDET
                     HRPVMFIYNGGPGSATMWLHMGTFGPKRIVLPDEDHQEGAPYSIVNNQYSLLDASDLV
                     FIDAPGTGFSRTFGKDRGKAFYGIDQDGQAFSHFIRKFLTKYGRWNSPKYIFGESYGT
                     PRSAVLSADLNGVDLNGVILLSQILSFDNSIDGPEANPGVDQAYALGLPTMAATAWHF
                     HKLANQPPALGPFLQEVEKYALTDYMEALLQGSELSDEQKHAVAEKLHGYTGLPVDYL
                     VRANLRVNGGEFSKELKLDEMTTVGRLDTRYQGPDMNPLSAETDYDPQSDAITSGWLA
                     AINTYLRQDLKYGAQETYFPSGRMGGDFNWDMHHRTPGFRGFSSDWSGPNVMPDLAYR
                     MKMNPKMKVMLAGGYFDLATPYFEGEYEMHHLPMPNSL"
     gene            1802..4351
                     /locus_tag="VHE33_05950"
     CDS             1802..4351
                     /locus_tag="VHE33_05950"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HVT97030.1"
                     /translation="MPRSLFRLFPGAVSAVLLSATLASAQHPANRIAQEIDSGIATQL
                     PGSLNPRIAAARDIGRLNASAPIEGITLWFQPTPEQKAELDALVEAQQTPGSPLYHAW
                     LTPAQYASRFGLSDADLARVQNWLESQGFNIDRVANSRTSITFSGTAAQVESAFQTQL
                     HHYQVGAETHFANATDLAIPAALSGVVRAVGNLNDFRPAPHVRRSTLQPSFTSSQSGA
                     HYLSPKDVGTIYDINAAWNAGYTGSGVTIVVVGQTAIVPADISAFQTAAGLTVKAPTI
                     TLVPNSGTSTVVQGDEAESDLDVEYAGGIARDATINFIYTGNAQNKGVFDALAYAVDN
                     RIGSIINISYGACEPNVSAGAFSQLEGIMEQAATQGQTVVASSGDSGSTACYAPTGNT
                     VTPAMKVLAVNYPASSAYAVGVGGTEFTDVTSSTHWQSASGSDVISSALSYIPETTWN
                     DDPITAQFGAQDALSAAGGGISTLAARPSWQTGVPGIPSGSFRLVPDVSLASSPNYAG
                     YLYCTSDQSSWNKGNGQAASCNAGFRDSSTQDLTIAGGTSFAAPIFSGMLAIITQKQG
                     GSGLGVANRTLYTLAANSSTYASAFHDITTGGNQCTAGASFCSSSGSAQYAAAAGYDE
                     ATGLGSVDFNNLMTAWAGTSGGGSGGGTGSASFALSATSASATRGSSTTSTVQVTPQN
                     SYAGTVHFSATSTGSLLTYGCYTISDTAVTGGTTNATLTIYTSQSACSTTSGVHAFTA
                     HGNPGVTTSRNNAPFGKSIPLGAAAFAGLLFVGFRKSRKAWTLFSCLAVAAMLGFASG
                     CGSQSSNSTTSNSTASTNNQVSTGSYIVTVVGADTTTSGITASTTLTLTVN"
BASE COUNT          806 a         1613 c         1116 g          826 t
ORIGIN      
        1 tgcagcgagt tcggcatggg cagatgatgc atctcgtatt cgccctcgaa gtacggcgtc
       61 gccaggtcga aatatccccc ggccagcatg accttcatct tcggattcat cttcatccgg
      121 tatgccagat cgggcatcac gttcggcccg ctccagtcgc tcgagaagcc ccggaaccca
      181 ggcgtccgat gatgcatgtc ccagttgaag tcgccaccca tgcgcccgct gggaaaatac
      241 gtttcctgcg ctccgtactt cagatcctgt cgcagatacg tgttgatcgc cgccagccac
      301 cccgacgtaa tcgcgtcgct ctgcggatcg tagtctgttt cggcgctcag cggattcatg
      361 tccggcccct gataccgcgt gtccagccgg cccaccgtgg tcatctcgtc gagcttcagc
      421 tcctttgaaa attcgccgcc gttcacgcgc agattcgcgc gcaccagata atccaccggc
      481 agtcccgtat atccgtgcag cttctcggcg acggcgtgct tctgctcatc gctcagttcg
      541 ctgccctgca gcagcgcttc catatagtcg gtcagcgcgt acttttccac ctcctgcagg
      601 aacggcccca gcgccggcgg ctggttcgcc agcttgtgga agtgccacgc ggtcgccgcc
      661 atggtcggca ggcccagcgc atacgcctga tcgacgccag gattcgcctc cggaccgtca
      721 atggagttat cgaagctcag aatctgcgac agcagaatca ccccgttcag atccacgccg
      781 ttcagatccg ccgacagcac cgccgaacgc ggggtgccgt agctctctcc aaagatgtac
      841 ttcggcgagt tccaccgccc atacttcgtc aggaacttgc gaatgaagtg gctgaacgcc
      901 tgcccgtcct gatcgatgcc gtagaacgcc ttgccgcgat ccttcccaaa cgtccggctg
      961 aaccccgttc ccggtgcatc gatgaagacc agatcgctcg catcgagcag cgaatactga
     1021 ttgttcacga tggaatacgg cgcgccctcc tggtgatcct catccggcag gacaattcgc
     1081 ttcggcccaa acgttcccat gtgcagccac atcgtcgccg agccgggacc gccgttgtag
     1141 atgaacatca ccggccgatg cgtctcgtcg gtcgcatcct tccggaagta cgcgacataa
     1201 aagattcgcg ccgtcggcgg cgcctccgcc ggatcggccg gcggcttcac gccggaattg
     1261 ggcagcaagc tgccatcaaa tcccagcgtc gcatcctgtt gatcgttcgc gcccaccgtg
     1321 atggtgcccg ccaccgcgcg gtacgccacc gcctgcccgc caatcgtcac cgacccttcg
     1381 gtcgtcgagt ccggcgcgcc caccgtcgag gcggcccccg gcttcgcctc tgccttctcg
     1441 ttcccctttt cctgtgcccc tgccacacag gaaaacgtgc atgtgactgc tacgaataaa
     1501 ggaagtaccg cggcccggct ccacgaacgc aaatccatgc tcacctcgtc cttttttgga
     1561 atgcccccga gtataaccgc actgcacgga taagaaccgg attcggcgtg ctctggtgat
     1621 tttcgggttc cttgcccgag tatgccgtca agatcgccgc ccgcggtcgc accggcggtc
     1681 cattccatgc atcctctcga aacgaatctc cacgaatgat cgtctaacta cccacaaagt
     1741 tgtactgatt tgcctggaac ctttgctctg cctcatttgt aattcttgta aagaggtccc
     1801 catgccccgg tccctgttcc gcctttttcc aggcgctgtc tccgcagttc tcctctcggc
     1861 cacgctcgcc tcggcccagc accctgccaa ccgcatcgcc caggaaatcg actccggcat
     1921 cgcgacccag cttcccggct ccctgaatcc ccgcatcgcc gccgcccgcg acatcggccg
     1981 cctcaatgct tcggcgccga tcgaaggcat caccctctgg ttccagccca cgcctgaaca
     2041 aaaggccgaa ctcgacgctc tggtcgaggc ccagcaaact ccaggctcgc cgctgtatca
     2101 cgcctggctg acgcccgcgc aatacgccag ccgcttcggc ctcagcgacg ctgatctcgc
     2161 ccgcgtccag aactggctcg aatcccaggg ctttaacatc gaccgcgtcg ccaacagccg
     2221 gaccagcatc accttctccg gcaccgccgc ccaggtcgag tccgccttcc agacccagct
     2281 ccatcactac caagttggcg ccgaaaccca cttcgccaac gccaccgacc tcgccattcc
     2341 cgccgcctta tccggcgttg tgcgcgccgt cggcaatctc aacgacttcc gtccggcacc
     2401 gcatgtccgc cgcagcacgc tccagccctc gttcacttcg agtcaaagcg gcgcgcacta
     2461 cctcagcccc aaagacgtcg gcacgatcta cgacatcaac gccgcctgga atgccggcta
     2521 taccggatcg ggcgtcacca tcgtcgtcgt gggccaaacc gcgattgtcc ccgccgacat
     2581 cagcgccttt cagaccgctg ccggcctgac cgtgaaagct cccaccatca cgctggtgcc
     2641 gaattccgga acgtccacgg ttgtccaggg ggacgaagcg gaatccgatc tcgacgtgga
     2701 atacgccggc ggtatcgctc gtgacgccac catcaacttc atctacaccg gcaatgctca
     2761 gaacaaaggt gttttcgacg cgctcgctta tgccgtcgac aacaggatcg gctccatcat
     2821 caacatcagc tatggcgcct gcgaacccaa cgtcagcgca ggcgctttct ctcagctcga
     2881 aggcatcatg gagcaggctg ccacccaggg acaaaccgtc gtcgcatctt ccggcgactc
     2941 cggctcgacc gcctgctatg ccccaaccgg taatacggtc acccccgcga tgaaagtgct
     3001 cgccgtcaac taccctgcca gcagcgcgta cgcggtcggc gtcggtggaa ccgaattcac
     3061 cgatgtaacc tcctccaccc actggcaatc cgccagtggc agcgatgtca tcagctccgc
     3121 gctctcctac atccctgaaa ccacctggaa cgacgacccg atcaccgccc agttcggcgc
     3181 gcaagatgcg ctctcggccg ccggcggcgg catcagcacg ctcgccgcgc gtcccagttg
     3241 gcaaaccggt gtccccggca ttccttccgg cagcttccgt ctggttccag atgtctcgct
     3301 cgcctcttcc ccgaactatg ccggttacct ctattgcacg agcgaccaat cctcgtggaa
     3361 caagggaaat ggacaagccg ccagttgcaa cgccggcttt cgcgactcct caacccagga
     3421 tctaacaatt gccggcggca ccagtttcgc cgcaccgatc ttctctggca tgctcgccat
     3481 catcacgcaa aaacagggag gcagcggtct cggcgtcgcc aaccggactc tctataccct
     3541 cgctgcgaat tcctccacct acgcctccgc gttccacgac atcaccacgg gcggcaacca
     3601 gtgcacggcc ggcgcatcgt tctgttcctc gtccggttcg gcgcaatatg cggccgcagc
     3661 aggctacgac gaggctactg gtctcggctc ggtcgacttc aataacctca tgaccgcctg
     3721 ggccgggaca tccggcggcg gcagcggtgg cggcacaggc agcgcttcct tcgcgctctc
     3781 tgcaaccagc gccagtgcca ctcggggcag ttccaccacc tcgaccgtcc aggtcactcc
     3841 gcaaaactcc tacgccggaa ccgtccactt cagcgccacc tccaccggct cgctgctgac
     3901 ctacggttgc tacaccatca gtgacaccgc cgtcaccggc gggacgacca acgccacgct
     3961 caccatctac acctcgcaga gtgcatgcag caccacctcg ggtgtgcacg cattcaccgc
     4021 gcacggcaac cccggcgtga cgacctctcg caataacgcg ccattcggga agtcgatccc
     4081 gctcggcgcg gctgccttcg cgggcctgct cttcgttggt ttccgcaaat ccagaaaggc
     4141 ctggactttg ttcagctgtc tcgctgtggc agcgatgctt ggattcgctt cagggtgcgg
     4201 cagccaatcg agcaacagca cgacctccaa ctccaccgcg tcgacgaata accaggtctc
     4261 caccggcagc tacatcgtca ccgtggtcgg ggccgacacc acaacctccg gcattaccgc
     4321 cagcacgacg cttaccctca ccgtcaacta gtgtgctgtc c
//
LOCUS       DASZYN010000232         2802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_391541, whole genome shotgun sequence.
ACCESSION   DASZYN010000232 DASZYN010000000
VERSION     DASZYN010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391541"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VHE33_05955"
     CDS             complement(<1..194)
                     /locus_tag="VHE33_05955"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HVT97031.1"
                     /translation="MDPALFEQMAVPLFDQLYNLAHWLTGNRTDAEDLVQETYAKALR
                     GFRSFEAGTNLRAWMYRILR"
     gene            complement(210..620)
                     /locus_tag="VHE33_05960"
     CDS             complement(210..620)
                     /locus_tag="VHE33_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVT97032.1"
                     /translation="MKPTTAHKIFAAGFAAWLLQAWMTIPVRSQSVAGLSQTARAGQL
                     LFEKRCTGCHGLDTAKEGPPLRTVYGRKAGSVAGFEYSDALKNSQMVWDDSKLNQWLS
                     NTDSLVKGNDMDFAVPKREERAEIIEYLKESSGK"
     gene            complement(617..1621)
                     /locus_tag="VHE33_05965"
     CDS             complement(617..1621)
                     /locus_tag="VHE33_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVT97033.1"
                     /translation="MTQTRESSPEPMLDASHNKISQDGVDRRGFLKCMAWAGTGLIWT
                     FSGGVPVSWAFGEPPRSGKSSDFSFVQISDSHIGFNKPANADVTATLQTALARIEAAP
                     AAPDFLIHTGDLTHSAKSAEFDTLSQVLGTRRQMFYVPGEHDTAVDDGKLYLSLFGQG
                     TQGNGWYSFDHKGVHFIGLVNVVQLEGMGRLGQAQLDWLKQDVHGLKSSTPIVVFAHI
                     PLWAVYPQWGWGTSDSEQALAMLRRFGSVTVLNGHIHQVLQKVEGQVTFHTAMSTAFP
                     QPAPGTAASPGPMKVPADKLRDLLGITEVQFVARRHTLAVVDSSLSGAPAKETKALVR
                     "
     gene            1789..2328
                     /locus_tag="VHE33_05970"
     CDS             1789..2328
                     /locus_tag="VHE33_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97034.1"
                     /translation="MLRRFSCLSLSLLVAAGFAVSVRAYAQNDTLTEDEVQEIRDNKV
                     NPNERIKLYLKFIDQRLDAIKQLSAAGKSSKEKGQIHDKFEEFTRLCDELQDNLDTYN
                     EAHADIRKSLKDLVADTARWPAILNAAGSDRSLEFSQKTALEAAKSAADQAHDLSLQQ
                     QVYFDVHKKERGGTGTGPR"
     gene            2402..>2802
                     /locus_tag="VHE33_05975"
     CDS             2402..>2802
                     /locus_tag="VHE33_05975"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HVT97035.1"
                     /translation="MNRLRTCLSALACAALVLGNSALNSQQAAPDLSFPTVLYGVAYY
                     NEYMPGDQDARLDKDVALMKAAGLNIVRMGESTWSLWEPEDGQFDYAWMDRIVDAMGK
                     AGIKVILGTPTYSIPAWMAHQHPEILADRIPG"
BASE COUNT          533 a          919 c          795 g          555 t
ORIGIN      
        1 ttgcgcagga tgcggtacat ccacgcacgc agattcgtgc ctgcttcgaa cgaacgaaag
       61 ccacgcagcg ccttcgcgta ggtttcctgc accagatcct cggcatcggt gcgattgccg
      121 gtgagccagt gggcgaggtt atacagttga tcgaacagag gcaccgccat ctgctcgaag
      181 agtgcggggt ccatccccct cccatccggt tacttccctg aggattcctt caaatactct
      241 atgatctccg cccgctcctc acgtttcggc acggcaaaat ccatgtcatt gcccttcacc
      301 agagagtcgg tgttgctcag ccattggttc agcttcgagt cgtcccatac catctgcgaa
      361 ttcttcagtg catcggaata ctcgaacccg gcgacgctac ccgctttgcg cccatagacc
      421 gtccgcagcg gcggtccctc cttcgcggtg tcgagaccat ggcagcccgt gcagcgcttc
      481 tcgaagagca actgtccggc cctggccgtc tgcgataacc cggccacgct ctgactccgg
      541 acaggaattg tcatccacgc ctgcagcagc catgcagcga atccggccgc gaagatcttg
      601 tgcgccgtcg tgggcttcat cgcaccagcg ccttcgtctc cttcgcagga gcgcccgaca
      661 acgaggagtc caccacagcc agcgtatgcc tgcgtgcaac aaactgaact tcggtgatgc
      721 cgagcaggtc gcgcagcttg tccgccggaa ccttcatcgg ccccggcgag gctgcagtgc
      781 caggagcggg ttgagggaaa gccgtggaca tcgctgtgtg aaatgtgacc tggccctcca
      841 ccttctgcag cacctggtgg atgtggccgt tcagcacggt caccgatcca aaacgccgga
      901 gcatcgcaag agcctgctcg ctgtcggagg ttccccagcc ccactgcggg tagacggccc
      961 agagcggaat gtgcgcgaac accacaatcg gcgtgctgct cttcaggccg tggacatcct
     1021 gtttcagcca gtccagctgc gcctgtccca ggcggcccat gccctccagc tgcactacgt
     1081 tgaccagtcc gataaagtgc acgcccttgt gatcgaagct gtaccagccg tttccctgcg
     1141 tgccctgtcc aaagaggctc aggtacaact tgccatcgtc gaccgcggta tcgtgttcac
     1201 cgggtacata gaacatctgc cggcgcgtgc ccaggacctg gctgagagta tcgaactctg
     1261 ctgatttggc cgaatgtgtc agatcgccgg tgtggatcag aaagtcggga gcagccggcg
     1321 ccgcctcaat cctggcaagg gctgtctgta gagtggccgt cacgtccgca tttgccggct
     1381 tgttgaagcc gatgtggctg tcgctgatct gcacaaagct gaagtcggac gacttcccgc
     1441 tgcgtggggg ctcgccgaag gcccacgaga cgggcacacc gccagaaaat gtccagatca
     1501 gcccggtccc ggcccacgcc atgcatttta gaaagccgcg ccggtccact ccatcctggc
     1561 tgattttgtt atggcttgcg tcgagcatcg gctcgggcga cgattcgcgg gtctgcgtca
     1621 cggtctctcc ctggggacgt tctctgctgc ccgtggatga aaaccgagtc agtccgcttt
     1681 tattcccttg tgctcgtaaa tttcgcctga tgaactcgaa tcctggatgg gtcctgtttc
     1741 ctcttccatc ccgcatccaa ttattctggt atctgccgag gtcgtgctat gttgcgccgc
     1801 ttctcctgcc tgtctctctc cctcctggtt gccgccggat tcgccgtgtc ggttcgggcc
     1861 tacgctcaga acgacactct gaccgaggac gaagttcagg agattcgcga caataaggtc
     1921 aatcccaacg agcgcatcaa gctctacctc aaattcatcg atcagcgcct cgacgccatc
     1981 aaacagctct ccgcagcggg caagagttcg aaggaaaagg gccagatcca cgacaagttc
     2041 gaggagttca cccgcctgtg cgatgagttg caggacaacc tcgacaccta caacgaggcc
     2101 cacgccgaca tccgcaaatc gctcaaggat ctggtggcgg atacggcgcg ctggcctgcc
     2161 atcctgaacg cagccggctc ggatcgcagc ctggaattct cgcagaagac cgcgctggaa
     2221 gccgccaaaa gcgccgcaga ccaggcgcac gacctctcgc tgcagcagca ggtctacttc
     2281 gacgtgcaca agaaggagcg tgggggcacc ggtaccgggc cgcgttagcg tttcctcatc
     2341 ggtcatctgt cccgggagcc gactccggtt tacacttgcc ctcggttcct gagggataag
     2401 aatgaatcga ttgaggacgt gcctttctgc gctggcgtgc gccgcgctgg tgttaggaaa
     2461 ttccgcgctc aacagccagc aggccgcccc cgatctcagt ttccccaccg ttctctacgg
     2521 cgtcgcttac tacaacgagt acatgcccgg cgaccaggac gcgcgcctcg acaaagacgt
     2581 ggcgctgatg aaagctgccg gcctcaacat cgtgcgcatg ggcgagtcca cctggagcct
     2641 gtgggagccc gaagacggcc aattcgacta cgcctggatg gaccgcatcg tcgacgccat
     2701 gggcaaggcc ggcatcaagg tcatcctcgg cacgcccacg tactccatcc cggcgtggat
     2761 ggcccaccag cacccggaga tcctcgccga tcgcatcccg gg
//
LOCUS       DASZYN010000233         3173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_844422, whole genome shotgun sequence.
ACCESSION   DASZYN010000233 DASZYN010000000
VERSION     DASZYN010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844422"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VHE33_05980"
     CDS             <1..744
                     /locus_tag="VHE33_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HVT97036.1"
                     /translation="VRVSPDGGTLVVTNRAGNSATVVDAEKLKVRAVFGGCPGASDAV
                     ILPDSSKAFVACTGGHQVMSIGLKRPESQNWAGQNPQERTDRLLAFVDVGAAPVHLAL
                     KPDGGEIFVSNFGGDTISEIATGANEVGGAYAVGSAPLGGVVSADNSTLWVSNSRDNT
                     VAAYSIDDGQLVNTVQVGDGPGPLALSGNGFLLLAVDRGAGDVSVVKTISYTAKGAPI
                     TGSLFTVLSAGKGPNAIAVKGFNVGAKGG"
     gene            complement(979..1881)
                     /locus_tag="VHE33_05985"
     CDS             complement(979..1881)
                     /locus_tag="VHE33_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT97037.1"
                     /translation="MNRYGRQDVLRILRISSRQLSAWERAGLIVPAADYGFQDLVQLR
                     KLRDLRAQRISASNIRAGLSAMKAVSGISNPLLQAGVALHGPRLVFRHSGATVDPIAG
                     QFEFDFEGGGSRLEIVEEAAVSETQRQTKINALFFEGVRCEEQGKIDEAAKLYEETLA
                     VGEHAPAAINLGTILYNKRQFVRAEQLYRRATLTNPDYALAFFDLGNALDELQRLPEA
                     VEAYTAAIRLSPGYADAHYNLALAYERSNERRRALRHWRAYIKLDPIGPWSNHARLQV
                     RKILDREELTIVWRRPKTALAKNC"
     gene            complement(2047..2589)
                     /locus_tag="VHE33_05990"
     CDS             complement(2047..2589)
                     /locus_tag="VHE33_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVT97038.1"
                     /translation="MSAIAAVAGSVKEGWAMQASAQQNQTPTMIVTPDEAQVYNMLYG
                     GEARLLVTGERSNGAWWMGRFREDPGFMTHLHYHPNTDEQVYVLEGVLSVYTDDRWHE
                     LTPGTLGVLPRGKPHAQGNRSEKPVHFIGTGEPAGFDKFFPAADALMKQKQPGSPEFI
                     AELQKIMAGCDIVSLGPAPK"
     gene            2770..>3173
                     /locus_tag="VHE33_05995"
     CDS             2770..>3173
                     /locus_tag="VHE33_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVT97039.1"
                     /translation="MAQRNRTRRAIVDAAMQLIAAGSTPSMAEVAAAAQVSRRTLYMY
                     FPTLEQLLIDATLGALSQNTVDAAMGASESRDPVERAMQLSRAINSHSAETLHLGRAL
                     IRLTVEGAEPSAGGPRRGYRRVQWIEEALKPAR"
BASE COUNT          582 a          818 c         1156 g          617 t
ORIGIN      
        1 gtgcgggttt ctccggatgg cggaacgctg gtggtgacga accgcgcggg gaacagcgcg
       61 acggtggtgg atgcggagaa gctgaaggtg cgggcggtgt tcggtgggtg ccccggcgct
      121 tccgatgcgg tgattctgcc ggattcgtcg aaggcgtttg tggcgtgcac gggcgggcat
      181 caggtgatgt cgatcgggct gaagcggccg gagagccaga actgggccgg acaaaacccg
      241 caggagagga ccgatcggct gctggcgttt gtggatgtgg gtgcggcgcc ggtgcacctg
      301 gcgctgaagc cggacggcgg cgaaattttc gtgtcgaatt ttggcggcga cacgatctcg
      361 gagatcgcga ccggcgccaa cgaggtgggt ggagcgtatg cggtgggttc ggcaccgctg
      421 ggcggagtgg tgagcgcaga taactccacg ctgtgggtga gcaattcgcg cgacaacacg
      481 gtggcggcgt attcgattga cgatgggcag ctggtgaata cggtgcaggt gggggacggg
      541 ccggggccgc tggctttatc cggcaatggc tttctgctgc ttgctgtgga tcgcggggcg
      601 ggggatgtgt cggtggtgaa gacgatcagc tatacagcga agggcgcgcc gatcacgggg
      661 tcgctgttca cggtattgtc ggcgggtaag ggtccgaatg cgatcgcggt gaaggggttc
      721 aatgtagggg cgaagggcgg ctagcgtggt gtttccgaaa tttctttatg cggaagagaa
      781 gcaggtccct ccgcttcccc ttcgctacgc tcagggtccg gtcgggatga caaggtaaag
      841 gaacttcaga aacaccacac tggcggaaag ctcctggctc ggaacgttta gccagaaacg
      901 gttagcaaac tgcgagcaga agagcgagcg ggaatgccta ccgttctttg cgaaacgcct
      961 tctgcgaatc ttgttttgct aacagttttt cgccagcgcc gtttttggtc tgcgccagac
     1021 gatggtgagc tcttcgcggt cgaggatttt gcggacctgg aggcgagcgt gattggacca
     1081 ggggccgatg ggatcgagct tgatgtaggc gcgccagtgg cgcagggcgc ggcggcgctc
     1141 gttgctgcgc tcgtaagcaa gagcgaggtt gtagtgcgcg tcggcgtagc cgggcgaaag
     1201 gcgaatggcc gcggtgtagg cttcgaccgc ttcaggcagg cgctgcagct catcgagggc
     1261 gttgccgaga tcgaagaagg ccagggcgta atcgggattc gttagagtgg cgcggcggta
     1321 gagctgctca gcgcgcacga actggcgctt gttatagagg atggtgccga gattgatggc
     1381 ggcgggtgcg tgttcgccga cggcgagggt ctcttcgtag agcttcgcgg cttcgtcgat
     1441 ctttccctgc tcttcacaac gaacgccctc gaagaagagg gcgttgattt tcgtctggcg
     1501 ctgggtttcg ctgaccgcag cttcttcgac gatctcgagg cgcgagccgc ctccttcaaa
     1561 gtcgaattcg aactggcctg cgatcggatc gacggtggcg ccggaatggc ggaagacgag
     1621 gcgcgggcca tgcagggcaa caccagcctg gagcagcgga ttggagatgc cggagacggc
     1681 cttcatggcg ctgaggccgg cgcggatgtt ggaagcggag atgcgctggg cgcggaggtc
     1741 gcggagcttg cggagctgaa ccaggtcctg aaaaccgtag tcggctgcgg ggacgatgag
     1801 gccggctctc tcccacgcgc tcaattgccg ggaggagata cgcagtatgc gaaggacatc
     1861 ctggcggccg tatcggttca ctgtcagggg tggtgacccc tccttgattc tccacgatta
     1921 tccagggcga actttgcgcg agcaagggcg gaaaacaaaa aagacgtgga taaatgagta
     1981 caaatctggt acgtgccgaa ctgggttcgt ggtgagggag aagcaggaag cagactagcc
     2041 ggctccttac ttcggagccg gtccgagcga gacgatgtcg cagcccgcca tgatcttctg
     2101 caactccgca atgaactccg gggaaccggg ctgcttctgc ttcatgagag cgtccgcggc
     2161 gggaaagaac ttgtcaaaac ccgcgggctc tccggtgccg atgaagtgca ccggtttttc
     2221 gctgcgattg ccctgggcgt gcggcttgcc gcgaggcagg acgccgagcg tgccgggggt
     2281 cagctcgtgc cagcgatcat cggtgtagac ggagagaact ccttcgagca cgtagacctg
     2341 ttcgtcggtg ttggggtggt agtggaggtg ggtcatgaat ccgggatctt cgcggaagcg
     2401 gcccatccac caggcgccgt tgctgcgctc gccggtgacg agcaggcgag cctcgccgcc
     2461 gtacagcatg ttgtagacct gcgcctcgtc cggcgtgacg atcatggttg gtgtttgatt
     2521 ttgctgtgct gaagcctgca tagcccatcc ttcctttacg gatcctgcca ctgctgcgat
     2581 cgctgacatg ataagaaacc tccttcggtc gaccatgagt ttgccttcct gttgccgctg
     2641 atccgatatt ctgtgttcat tgcacatgtg tgcaatgatt gcacttgtgt atacttacat
     2701 cgattggcaa aaaaggtcaa caggaatcga aaaaattcgc agcctgcggc ggcgtccggg
     2761 gagggccgcg tggcacagcg gaatcgcacg cgccgggcga tcgtcgacgc ggcgatgcag
     2821 ctgatcgcag cggggagcac gccgtcgatg gccgaggtgg ccgcagcagc gcaggtatca
     2881 cggcgcacgc tgtacatgta cttcccgacg ctggaacaac tgctgatcga tgcgacgctg
     2941 ggggcgctga gtcaaaacac ggtcgatgcg gcgatgggcg catcggagtc gcgtgatccg
     3001 gtggaacgcg ccatgcagtt gagccgggcg atcaacagcc actcggcgga gacgcttcat
     3061 ctgggaaggg cgctgattcg gctgacggtg gaaggggccg aaccttcggc gggcggaccg
     3121 cgacgcgggt atcggcgagt gcagtggatc gaggaggcgc tcaagccggc acg
//
LOCUS       DASZYN010000234         1615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1036515, whole genome shotgun sequence.
ACCESSION   DASZYN010000234 DASZYN010000000
VERSION     DASZYN010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036515"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="VHE33_06000"
     CDS             complement(<1..1331)
                     /locus_tag="VHE33_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HVT97040.1"
                     /translation="MEPPARRRLTRAPLGLFQFILLLFFVLASFIPDARASRAVSFNI
                     STNRTFSPDEKPTIHLYAHDVDELEFRVYRVNDAEKFLTSLPDLHSFGNAYPNGPREQ
                     IDERTWLEKFHDWKHHLWYLIRHFFRGQFSQETRDAFRAKQSSLAKRSRIVGVAQFAQ
                     IPLLNDRQLVARWRQEMPPTYISDSQDLPIDKLSAGLYLVEATDGHYKAYTLLMVSRM
                     VLITRTSTGTILAFVADRQTGTPVSKAQIVWGIGRKEQGRATTDSDGVAELRGAPSKQ
                     SEEDSLWITASTGNEFAAVTPMAWSFNNTATSKWASYVYTDRPVYRPGHTVHWKALLR
                     ARVENHLELPKIPAMHVRIADEQDHPVFDQQMPVSADGTVSGDITIAAGAALGYYTIR
                     LGDNDTEISGSFRVEEYRKPEYQVRVAAQNLRVLQGQSNQITIDSRYFFGEP"
     gene            complement(1304..>1615)
                     /locus_tag="VHE33_06005"
     CDS             complement(1304..>1615)
                     /locus_tag="VHE33_06005"
                     /inference="COORDINATES: protein motif:HMM:NF018387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HVT97041.1"
                     /translation="ADARALLALNTYPVSRNIHTALPGDLLFFRQLEQNSQYHSMVVA
                     GANADWVIYHTGPIGKQKGEMRRVSMEDLLHHPDARWRPVPGNTNFLGVYRWNLLHDG
                     D"
BASE COUNT          311 a          433 c          545 g          326 t
ORIGIN      
        1 ggttcgccga agaagtagcg cgagtcaatc gtgatttggt tggactggcc ctggaggacg
       61 cgaaggtttt gtgcagcgac ccgcacctgg tactcaggtt tgcggtactc ctcaacgcgg
      121 aagctgccgg agatttccgt gtcgttgtca cccagacgga tggtgtagta gccgagggcc
      181 gccccggcgg caatcgtgat gtcgcccgat acagtgccgt ccgctgagac aggcatctgc
      241 tgatcgaaga cggggtggtc ctgctcgtcg gcgatgcgca cgtgcatcgc cggaatcttc
      301 ggcagctcga gatgattctc gacacgcgcg cgcagcaaag ctttccagtg gacggtgtgg
      361 ccgggccgat agacagggcg gtcggtgtaa acgtaagagg cccatttgct ggttgctgtg
      421 ttgttaaagg accaggccat tggcgtgacc gcggcaaatt cgttgcccgt ggaagccgtg
      481 atccacaaac tgtcctcttc ggattgtttg gaaggagcgc cgcggagttc ggccacaccg
      541 tcgctatcgg tcgtggcccg gccctgttct ttgcggccga tcccccagac gatctgtgcc
      601 ttcgagactg gcgttccggt ctggcgatca gccacgaagg ccagaatcgt gcccgtgctg
      661 gtgcgggtga tcagcaccat gcggctgacc atgagcagcg tatacgcctt gtagtggccg
      721 tcggtcgctt cgacgagata gagcccggcg gacagcttat cgatcggcag gtcctgtgaa
      781 tccgagatgt aggtcggggg catctcctgg cgccagcgcg cgacaagttg ccgatcgttc
      841 agcagaggaa tctgcgcgaa ctgcgcaacg ccgacgatgc ggcttcgttt ggcgaggctc
      901 gattgtttgg cgcgaaacgc gtcgcgggtc tcctgcgaaa actggccgcg gaagaaatgc
      961 cggatcaggt accacaggtg gtgtttccag tcgtggaatt tttccagcca cgtccgttcg
     1021 tcgatctgtt ccctggggcc attgggatac gcattgccga aagagtgaag gtcgggaaga
     1081 ctggtcagga acttttcggc atcgttcacg cggtagacgc ggaactccag ctcatcgacg
     1141 tcgtgtgcgt agagatgaat ggttggcttc tcgtccggcg agaaggtgcg gttggtgctg
     1201 atgttgaagg agacggcgcg tgaggcgcga gcgtccggga taaaactcgc gaggacgaaa
     1261 aaaagcagca ggatgaattg gaacaggccg aggggagcgc gagtcagtct ccgtcgtgca
     1321 ggaggttcca ccgatagacc cctaagaaat tcgtgttgcc ggggacgggg cgccagcgcg
     1381 cgtcgggatg gtggagcaag tcctccatcg agacccgacg catttcgccc ttttgtttgc
     1441 cgatcgggcc ggtgtgataa atcacccagt ccgcattcgc tcccgcaaca accatcgaat
     1501 gatactgcga gttctgctcc agctggcgaa aaaacaacag gtcgccgggc agagcggtgt
     1561 ggatgttgcg ggaaaccggg tacgtattca gggcgagcag cgcacgggca tcggc
//
LOCUS       DASZYN010000235         2753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_716648, whole genome shotgun sequence.
ACCESSION   DASZYN010000235 DASZYN010000000
VERSION     DASZYN010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716648"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(327..>2753)
                     /locus_tag="VHE33_06010"
     CDS             complement(327..>2753)
                     /locus_tag="VHE33_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT97042.1"
                     /translation="STADSLLQDLRFAVRQLRKNPGFATTAILVLALGIAASVAIFAF
                     VDAALIRPLPYSDPSRLVILFESNSLGPRFHVSYKDYVDWKNQNTVFSSLDEFAPYGF
                     MLQTPTGTQKVEGARITAGFLRTLGVAPILGRDFRTNDDQPNAPRSVLLSYSEWESRF
                     GGRPDAIGQTVILDNQPNTVIGVMPRDFHFQPAEPADFWTTERADGGCEKYRNCHNAF
                     AIARLRPGVTLSAALANMKTIVAQLERQYPDSNRGRGINIVAASEVIVGDIRTVLLVL
                     LAGAALLLLIATINVASLLLVRSEGRRREIAVRGAIGASRVRLARQFVTEGFVLALSA
                     GLGGTALAAFVMRSFVALIPKAMLATMPYFATMNLNVRVLLFAVLVSFASALLFSIIP
                     VFRIRLADLRDGLTETARGSSGTLWRRLGSNLVVAELAVAVVLLTGAGLLGKSFYRLL
                     HADTGLDPDHLATLRVSADSPAYSKDAAVIALKKEIEARARALPGVRSVAFASRLPLG
                     DGDGTIILNVLGQAHEYGLREVAHREISTAWFSTIQAQLMGGRYFTEDDDASHPLVAI
                     VNQALARKFFPGQDPIGRVVFSEGNPKAHVHIVGVVNDIQEGQLDAPPRAALYIPFYQ
                     TPQSDFAVVLRTSQPGDALLSSLAAAVHSIDPGVATFEPSTMDERLRDSPSAWLHRSS
                     AWIVGGFAALALLLSIVGLYGVIAYSVSRRTREIGVRMALGAQRSAVYRLILREAARL
                     AVIGIAAGLLCALAATTLLRSLLFHVQSWDLATLGAVALVLGSAALLASFLPARRAAS
                     VNPTEALHAE"
BASE COUNT          546 a          762 c         1026 g          419 t
ORIGIN      
        1 tttgagcagg gcctcccagc cgctggaatg gcgtggaatc ctggggccgg tgagaacggt
       61 gggcccgcgc ctctcctctt ccttttttct gccgaacagg ttgaccatgc ggagcgcaga
      121 accctcgttt ttggattgta acggaggcag cgggtgggct gggtccggca ggggattgag
      181 cgggtggaca acgagcagaa aaccgagtga aaagcgagtc aagagcgaga ggaatcgagc
      241 ggggcaggct gatgtggctg ggccgctcgc ggcaatcagg ctcatgtccc caaatccagg
      301 gactgagggc tcgggtgccc gggcccctat tcggcgtgga gcgcttcggt ggggttcacc
      361 gacgcggccc gccgggcggg caggaagctg gccagcagcg ccgccgaccc caggacgagc
      421 gcgacggcac ccagggtggc aaggtcccag ctctggacgt gaaacagcaa agagcgcagg
      481 agcgtggtgg ccgccagggc gcagagcagt cctgcagcga ttccaataac agccagccgg
      541 gccgcttcgc gcagaataag ccggtaaacg gctgagcgtt gcgcccccag agccatgcgt
      601 acgccgattt cgcgggtgcg gcgggaaacg gaataggcga tcacgccgta gagccccacg
      661 atgctgagca gaagcgccag agcggcgaat ccaccgacga tccaggccga ggagcgatgg
      721 agccaggcag agggggaatc gcggaggcgc tcgtccatgg tggagggctc gaaggttgcg
      781 acgcccggat cgatgctgtg tacggctgcg gcgagcgagg agagcagcgc gtcgcccggc
      841 tgcgaggtgc gaagaaccac agcgaagtct gactgcggcg tctggtagaa ggggatgtaa
      901 agcgcggcgc ggggtggagc gtcgagctgg ccttcctgaa tgtcgttgac gacgccgacg
      961 atgtgcacgt gcgccttcgg gtttccttcc gagaacacta cacggccgat gggatcctgg
     1021 ccgggaaaga acttcctggc cagcgcctga ttgacgatcg ccaccagcgg gtggctggcg
     1081 tcatcgtctt cggtgaagta gcggccaccc atgagctgcg cctggatggt ggagaaccag
     1141 gcagtgctga tttctcggtg tgcgacttcc cgaagcccgt actcgtgcgc ctggccgaga
     1201 acgttgagga tgatcgtccc gtctccatcg cctaagggga gacggctggc gaaggcgacc
     1261 gagcgaacgc cgggcagagc gcgggcgcgg gcctcgattt ccttcttgag agcaatgacg
     1321 gcggcatctt tggagtaggc gggactgtcg gcgctgacgc gcagcgtggc caggtgatcg
     1381 ggatcgagcc ctgtatccgc gtggagaagg cgatagaagc tcttgcccag aagcccggcg
     1441 ccggtgagca gcacaaccgc gaccgccagc tcagcgacga cgaggttcga acccaggcgg
     1501 cgccacagag tgcctgaaga tccgcgcgca gtttccgtca ggccatcgcg gaggtcggcg
     1561 agccgaatgc ggaacacggg aatgatcgag aagagcaacg cggaggcgaa ggagacgagg
     1621 accgcgaaga gcagcacccg aacattgagg ttcatggtcg cgaagtacgg catggtcgcg
     1681 agcatcgctt tggggatgag ggccacgaag gaacgcatga cgaaagcggc gagcgcggtg
     1741 ccgccaaggc cggccgagag cgcgagcacg aatccttcgg tgacgaactg gcgggcgagg
     1801 cgaacgcgtg aagcgccgat ggcgccgcga accgcgatct cgcggcggcg gccttccgat
     1861 cgaaccagga gcaggctggc cacgttgatg gtggcgatca gaagcagaag tgcggcaccg
     1921 gcgagaagga ccagcagcac ggtgcggata tcgccgacga tgacctcact cgccgccacg
     1981 atgttgatgc cgcggccgcg gttgctgtcc ggatactggc gttcgagctg cgccacgatg
     2041 gttttcatgt tcgccagggc ggcggagagc gtgacgccgg gacgaagacg ggcaatcgcg
     2101 aaggcgttgt gacagttgcg atacttctcg cacccgccat cggcgcgctc ggtggtccag
     2161 aagtcggcag gctcggcggg ctggaagtgg aagtcccgcg gcatcacacc gatgacggtg
     2221 tttggctgat tgtccaggat gacggtttgc ccgatcgcgt ccggacgccc gccgaagcgg
     2281 ctctcccatt cggaatagct cagaagaaca gagcgcggcg cgttaggctg atcgtcgttg
     2341 gtgcggaagt ctcgaccgag tatcggtgcg acgcccaggg tgcggaggaa tcccgcagtg
     2401 atgcgtgcgc cttcgacttt ttgtgtgccg gtaggggttt gcagcatgaa accgtagggc
     2461 gcgaactcat ccagtgagga gaagacggtg ttctggtttt tccagtcgac gtagtcctta
     2521 tacgacacgt gaaaacgcgg gccgagagag ttgctctcga agaggatgac cagacgcgag
     2581 gggtcagagt agggcagcgg gcggatgaga gccgcatcga cgaacgcgaa gatggcgacg
     2641 ctggcggcga tgccgagggc caggacgagg atggcggtgg tggcgaagcc gggattcttg
     2701 cgaagctggc gcacggcgaa acgcaggtcc tggaggagcg agtcggcggt aga
//
LOCUS       DASZYN010000236         7272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1163350, whole genome shotgun sequence.
ACCESSION   DASZYN010000236 DASZYN010000000
VERSION     DASZYN010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163350"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1480
                     /locus_tag="VHE33_06015"
     CDS             <1..1480
                     /locus_tag="VHE33_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97043.1"
                     /translation="PTQEDWYWGRLATGNEEDFWWRRLSDNWYMKDVIPSTYLELHNQ
                     CYEAYNANPLAFAIIEITTSFVLGKGVKVTTRNRKVQAILDGFWHDPDNRMESRIYDI
                     CTELGLYGEIFVRFFVNRFDGRVKIRMIDPSIIDQVETDPDDIETQLRFHQRPIGPSP
                     TQNVDSINIINPNVINAQNPGDPTMAGKWFEAGTEVVHFAINKVSNAKRGKSDLATLL
                     PWLRRYKDWLTDRVRINKYKSAFLWDVTLKGADLKTINAKKMEYSYPPEPGSVIIHNE
                     AEQWQAVKPEINAQEAEKDGRAIKLMVAVGATLPEHYLSDGENNNRATSENMGLPTFL
                     KFKRRQNVIKNILRTILDRVLQEAQRVGRLSRTVDITKAYDLIFPEFDHEDNQEMGTA
                     ISYLMQGLTAAKGAGWISNETAMKVLFQFCDLEIDVNEEADRIAAEQGQPRARTTQAP
                     GRKRTEPGQVHGPDETQQPRESPELAGKIKGNTIGGVGPGGW"
     gene            1672..2055
                     /locus_tag="VHE33_06020"
     CDS             1672..2055
                     /locus_tag="VHE33_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97044.1"
                     /translation="MPLDARKIAHIRAAVQRTWIGRQTTVVFVSNAGGTYSYTAQSVI
                     WKPQQVIDPQIPNVSGAPPGIPADVVMIVPLTTSLIGVVFVAFTNTATSGAVAAAQKF
                     ELIEAVPTGILPGGTHYTVALRRFR"
     gene            2296..3066
                     /locus_tag="VHE33_06025"
     CDS             2296..3066
                     /locus_tag="VHE33_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97045.1"
                     /translation="MDEPLYPSVQGQSIPDLGLAQYGVATPQTHLSTTSDSTPPMMCV
                     PELMGDARSIHQMGIDPEVPYYHLELANDHKIMTPAQLAAPQGIGDVNAPTFDAPEMG
                     VPGLRAGDLTGPGIDHVGEFEPDPHTGDLLQFAQPGGLEIHAASTRLTSDPMLPDLGE
                     YDRPAGLEMLNEMMPDPTLPDLQQPQLEQNVHMIGRPGDLASDAMKAGADEVEKEDVP
                     SGDYKEMWMQQHGRATHRTRHMKMLTTGLHEEGDHDYR"
     gene            3063..5459
                     /locus_tag="VHE33_06030"
     CDS             3063..5459
                     /locus_tag="VHE33_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97046.1"
                     /translation="MRIRESTATLEDIQRIYEARWTDRARRAYFATHPHRFADPERMA
                     YPIKDASDVKDAARLVGNGKGSKEQIKAAIIRIAREMNLEHALPESWKAGKVRESIQI
                     LGQICEAQENESTTGHEVRVTVVKGGRSINGFYYDRNALVQIAGMLEGARAYADHGRS
                     AADETTRSVRDMVGFYKHAQYIPNDPSTPIGRVDATLHIMEAADWLWSMISEAIKIGK
                     PDLVGVSIDIFGVAEHDATLKARHVTNVLSLNSCDVVTRPSAGGAFQRILHDYTEEGE
                     REDMDPKKGTTAEKGRGAAGSQVDDPPDSEQQDQDQQSQQAQNITESQNNGGAAPGGS
                     TEGHTQPSPTQQIQEAESKRILETLKTERCGLILERKLQESILPQAACERIKASFAGR
                     IFEESELEREITSMQTMLASMTDAGLIRGHGYEKPHIEGQITEAEKVQAAFDVMMGVD
                     IDTAKLGNIRGFSSIREAYLRVTGDSNLAGGISGQSRLGQIAISESAPITRINEADTT
                     TASFSYLLGTSMNKRLLKDYQAWPAEWQKFCVIAPIKDFRQQSRIRFGAFGSLSTVAE
                     DTAYTSITLQDTAATYVPTKRGNLVTISRETIVNDDLQAIKQIPTKLAVAAAYTLAEF
                     VYAFLSGNPTIYDGNALFTSGAPHSNLGSAALSSAAMQAGVTAMREQTNMAGKRLGLK
                     PSFLIVPAELEWVAMVATKSAGVPGSANNDINPMLGYVTPIVSPQLTNTSQWFFAADP
                     RVVDTIEIGFVGGQVNPVLFIQDQPLFGLNFTQDIISYKIRHEYGGAVLDYRGLYRGI
                     "
     gene            5537..6019
                     /locus_tag="VHE33_06035"
     CDS             5537..6019
                     /locus_tag="VHE33_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97047.1"
                     /translation="MAQIPNTRALTASTDGSDAGTVFNVNTATKQVQIPNGASIQFYS
                     DAYATATGQLTGSGPVSAMSATSPDPGAGGTIATAGVGVALVTPAAARTGIILQAGTV
                     NGQEVWVVNQGAAANTLTFHTTPATSNVADSATEPAIAGLKARKYVWVSAVSLWYPAQ
                     "
     gene            6059..6370
                     /locus_tag="VHE33_06040"
     CDS             6059..6370
                     /locus_tag="VHE33_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97048.1"
                     /translation="MKTPKATRPTRIVKPGDIVNYVLSSGRGKGEIRPAIIVRLWGEA
                     TADSTPAAQLQVFTDGTNDFDHGQPGSLTGLLWATSVSHDEAEKKPGTWHWPEAVEGE
                     A"
     gene            6370..>7272
                     /locus_tag="VHE33_06045"
     CDS             6370..>7272
                     /locus_tag="VHE33_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97049.1"
                     /translation="MAGFLTQDGAELAGGLNPNNIGQALRVDANGYLLTAQAGASNSP
                     ANYTASGSATNDGDTVSIAVQDTETALVQILGAFTMTLLFEASPDNGTTWYSVSGVQG
                     TGTVASSATAPGYWRVNVAGFTNFRIRAHPITSGTATITIRLTQAVNNVSIANAQPLG
                     QQTSANSSSVVIASDQTVPVKGGTVEIIGNGTGVVNGVNTDLISSTDVSAYKWLSLQI
                     QGTYNATLSFQASNDNVNWVAVKLILFTSYVDNNTSSSTTSTGQIWCGPVTFRYFRVR
                     ATSFTSNTSLAGTLELYAMAPTLPI"
BASE COUNT         1779 a         2200 c         2104 g         1189 t
ORIGIN      
        1 ccctactcaa gaagattggt actggggacg gctcgcaaca ggtaatgaag aggatttttg
       61 gtggcgccgc ctctccgaca actggtatat gaaggatgta attccttcta cctatttgga
      121 attgcataac cagtgctacg aagcatacaa cgccaatccc ctggccttcg ccatcatcga
      181 aatcaccacc agctttgtgc ttggcaaagg tgtcaaggtg acaactcgaa atcgcaaagt
      241 gcaggccatt ctcgatggct tctggcatga tcccgacaac cgcatggagt cgagaatcta
      301 cgacatctgc accgaattgg gcctctacgg cgaaatcttt gtccgcttct tcgtcaatcg
      361 cttcgatggc cgggtcaaga tccgtatgat cgacccctcc atcatcgatc aggtcgagac
      421 ggaccccgac gacatcgaaa cgcagttgcg gttccaccag cgacccatcg gcccatcgcc
      481 gacgcagaac gttgacagca tcaatatcat caatcccaac gtcatcaacg cgcagaatcc
      541 cggcgatccg acgatggccg gcaaatggtt tgaggccggg actgaagtgg tccatttcgc
      601 gatcaataag gtcagcaacg ccaagcgcgg caagagcgac ctggccaccc tgctgccctg
      661 gctccggcgt tataaagact ggctcaccga ccgggtgagg attaacaagt acaagtctgc
      721 gttcttgtgg gacgtgacgc tcaaaggcgc agacctgaaa accatcaacg ccaaaaagat
      781 ggaatattcg tacccgccag agccaggctc ggtcatcatc cacaacgaag ccgaacagtg
      841 gcaggctgtg aagccggaga tcaacgccca ggaggccgag aaggacggca gggccatcaa
      901 gctgatggtg gcagtggggg cgaccctgcc ggagcactac ctgagcgatg gagagaacaa
      961 taatcgagcc acctccgaga acatggggct gccaaccttc ctcaaattca agcgccggca
     1021 gaacgtcatc aaaaacatcc tgcgcacgat tctggatcgc gtcttgcagg aggcgcaacg
     1081 cgttggcagg ctctctcgta cggtggacat cacgaaagcc tatgacctga tcttccctga
     1141 gttcgaccat gaggataatc aggaaatggg caccgccatc agctatctga tgcagggact
     1201 cacggcggca aagggcgccg gctggatctc caatgaaacg gccatgaaag tgctctttca
     1261 gttctgcgat ctggaaatcg atgtcaacga ggaagcagac cgcattgcgg cagagcaggg
     1321 gcagccacgc gcaagaacaa cacaggcgcc aggcagaaag aggacagagc cagggcaggt
     1381 ccatggtcct gatgagacac aacagccgcg cgagtcgcct gaactggcgg gcaagatcaa
     1441 gggcaacacc atcggcggcg ttggcccagg aggatggtaa tgcgacaccc catcaacggc
     1501 cagatccgca acggtcaggc accgggaaac agcgcaggca atgatgcagc cgcgcaggtc
     1561 aggccatcat caagcccgac cagcgcccgc catgagggcc atcctcatgc gcgcgagcac
     1621 ccgcccggct atcggcctgc ccgctcccac gaccggaaag gagcctgacc gatgccactc
     1681 gatgcgcgca agatcgcgca cattcgggcc gcagtgcaac gaacctggat cggcagacaa
     1741 acaacggtcg tgtttgtctc caacgcagga ggcacgtaca gctatacggc gcagagcgtg
     1801 atctggaagc cgcagcaggt gattgatccg cagattccga acgtgagcgg agcaccgcca
     1861 ggcatcccgg ctgatgtggt gatgattgtg ccactgacta ccagcctgat cggcgtcgtc
     1921 tttgtggcct tcaccaacac cgcgaccagc ggcgccgtgg cggcggcgca gaagtttgaa
     1981 ctcatcgaag ccgtacccac cgggattctc cctggaggca cgcattacac ggtcgcatta
     2041 aggaggttcc gatagtggca gagccaggat cgaaaaagca gtggttagcc atcgccaggg
     2101 atgaccagct cagaggcagc cacctcttca ttgtggaggc tccgacacac aaagccgcct
     2161 atgaagcatg tactcatcaa tgcgtgctga accatctgag catccccgat caacgggcgg
     2221 tctacgaact gacagaagca agcggcgcgc acatctgtac agtgctcgac gcgaaaccaa
     2281 aaggaggact tttcaatgga cgagccgctt tatccctcag tccaggggca atccatacca
     2341 gacctgggat tggcgcagta cggcgttgcg acgccgcaga cgcacctctc caccacgagc
     2401 gacagcaccc cacccatgat gtgcgtaccc gaactgatgg gagacgcgcg gagcattcac
     2461 cagatgggta ttgatccaga ggtgccctat taccatctgg agcttgccaa cgatcacaag
     2521 atcatgacgc ccgcgcagct cgcggcgccg cagggcattg gtgatgtgaa cgcgccaacg
     2581 ttcgacgcgc cagagatggg cgtgccaggc ttgcgcgcgg gcgatctcac cgggccgggc
     2641 atcgatcacg tcggcgagtt cgagcctgat ccgcacacgg gcgacctgct tcagttcgcc
     2701 cagccaggcg ggctggagat tcacgcggcg tccacccgac tcacgtctga cccgatgctc
     2761 cccgacctcg gcgagtacga cagaccagcg ggcctggaga tgctcaacga gatgatgcct
     2821 gatccaacct tgccagacct ccagcagcct cagctagagc aaaatgtcca tatgatcggg
     2881 cgaccgggtg atctggcgtc agatgcaatg aaagcaggag ccgatgaggt cgagaaggaa
     2941 gatgtgccgt caggagatta caaagagatg tggatgcagc aacacggcag agccacgcac
     3001 cgcacccggc acatgaagat gctcacgaca ggactccatg aagaggggga tcacgattac
     3061 cgatgaggat acgcgagagc accgcaaccc ttgaggatat ccagcgcatc tacgaggccc
     3121 gctggaccga cagggccaga cgcgcgtact ttgccacgca tccgcaccgc ttcgccgatc
     3181 ctgaacgcat ggcgtatccc atcaaggacg ccagcgacgt gaaagatgcc gcgcggctgg
     3241 tgggcaacgg caagggcagc aaggagcaga tcaaagcggc gattatccgc atcgcgcgag
     3301 agatgaacct ggagcatgcc ctacctgaaa gctggaaagc cgggaaggtg cgagagagca
     3361 tccagatcct gggccagatc tgcgaagcgc aggagaacga aagcacaacc ggccatgagg
     3421 tacgggtcac cgtcgtcaaa gggggccgca gcatcaacgg cttctactat gatcgcaacg
     3481 ccctggtgca gatcgctgga atgctggagg gcgcccgcgc ctacgccgat catggccgta
     3541 gcgcagcaga cgagacgacc cgatcagtac gtgatatggt cgggttttat aagcatgccc
     3601 aatacatccc caatgatccg agcacgccca taggccgggt cgatgccacg cttcacatca
     3661 tggaagccgc cgactggctc tggagcatga tttccgaagc catcaagatc ggcaaaccag
     3721 atcttgttgg cgtctccatc gacattttcg gcgtggccga gcatgacgcc acactcaaag
     3781 cccgccacgt gacgaacgtt ctttcgctca acagttgcga tgtggtgaca cgcccaagcg
     3841 cgggcggcgc attccagcgc attttacatg attacaccga ggaaggagag agagaagaca
     3901 tggaccccaa aaagggtacg acagcagaga agggcagagg cgcagcaggt tcgcaggtgg
     3961 atgatccgcc cgacagcgag cagcaagacc aggaccagca gagccagcag gcgcagaaca
     4021 tcaccgagtc tcagaacaac ggaggcgcgg cgcctggagg cagcacagaa ggccatacac
     4081 agccctcgcc gacacagcag attcaagagg ctgagtcaaa gcgcattctg gagacattga
     4141 aaacggagcg ctgcggcctg atccttgagc gcaaactgca agaaagcatt ttgccccagg
     4201 cggcctgcga gcgcatcaaa gccagctttg ccgggcgcat cttcgaggaa agcgaactgg
     4261 agcgcgaaat caccagtatg cagaccatgc tggccagcat gaccgacgct ggcctgattc
     4321 gcggccacgg ctacgagaaa ccgcacatcg aaggccagat caccgaggca gagaaggtac
     4381 aggcagcttt cgatgtgatg atgggcgtgg acatcgacac ggcaaagctc ggcaacattc
     4441 gcggcttctc ctccatccga gaggcgtatc tgcgcgtgac cggagatagc aaccttgccg
     4501 gtggtatcag cggccagagc cgcctgggcc agatcgctat ctcagaatcc gcgcccatca
     4561 cacgcatcaa cgaggcagac acgaccacag cgagcttctc gtacctcctg ggcacgtcga
     4621 tgaacaaaag actgctgaag gactatcaag catggccggc tgagtggcag aaattctgtg
     4681 tgatcgcgcc gattaaagat ttccgccagc aatcgcgtat ccgcttcggc gccttcggtt
     4741 ccctctcaac ggtcgccgaa gatactgcct acaccagcat caccctgcaa gatacggcgg
     4801 ccacctacgt tcccacgaaa cgaggcaatc tggtcacgat ttccagggaa accatcgtga
     4861 atgacgatct ccaggcgatc aagcagatcc ccaccaaact ggctgtggcg gcggcataca
     4921 cgctggccga gttcgtctat gctttcttga gcggcaaccc gaccatttac gatggtaacg
     4981 cgcttttcac atccggcgca ccacacagca atctgggcag cgctgcgctg agtagcgctg
     5041 ccatgcaggc aggggtaact gccatgcgcg aacagaccaa catggccggc aagcgcctgg
     5101 gcctcaagcc aagctttctg attgtcccgg cagagttgga atgggtagcg atggtggcga
     5161 caaaatcggc aggcgtgcca ggctcggcca acaacgatat caacccgatg ctggggtatg
     5221 tgacgccgat tgtctcgccc caactcacga acacatctca atggttcttc gccgccgatc
     5281 cgcgcgtcgt cgataccatc gaaatcggct ttgtcggtgg acaggtcaac ccggtactgt
     5341 tcattcagga tcaacccttg tttggtctga actttaccca ggacattatc agctacaaaa
     5401 tcaggcacga gtacggcggc gcggtcctcg actatcgcgg cctctatcgc ggtatctagc
     5461 tgatctacca ggaggagaaa caatgggccg ccaggctgcg cagtctggcg gcccgcagac
     5521 aaaggagaag cgatacatgg cacagatacc gaacacgcga gcattgaccg ccagcacgga
     5581 cggcagcgat gcaggcacag tcttcaatgt gaacaccgcg acaaagcagg tgcagatccc
     5641 gaatggcgcg agcatccagt tttacagtga cgcctacgcc accgcaacag gtcaacttac
     5701 cggcagcggg ccagtttcgg cgatgagcgc aaccagcccg gaccccggcg caggaggcac
     5761 gattgcgacc gcaggcgtcg gggtcgcgct ggtcacgcct gcggcggcgc gcactggcat
     5821 cattctccag gccggaaccg tcaacgggca ggaggtatgg gtcgtcaacc agggcgcagc
     5881 ggccaatacc ctgacctttc ataccacgcc ggcgacctcg aacgtggccg actccgcgac
     5941 cgagccagcc atcgctggat tgaaggccag gaaatatgtc tgggtgagtg cagtcagcct
     6001 ctggtatccc gcgcagtaag cagagaaaac agcacaggag aagaacaaca ccatgaacat
     6061 gaagacacca aaagcgacga ggcccaccag gatcgtgaag cctggggata tcgtcaacta
     6121 tgtcctgtca agtgggcgcg gcaaaggcga aatacgcccc gccatcattg tgcgcctgtg
     6181 gggcgaagcg accgcagact ccacccctgc tgcacaattg caggtcttca ccgatggcac
     6241 caatgacttc gaccacggcc agccagggag cctcaccggt ctgctctggg caacatcggt
     6301 ctcacacgac gaagcggaga agaaaccggg cacctggcac tggccagaag ccgtggaggg
     6361 ggaagcctga tggcagggtt cctcacccag gatggcgctg aactggctgg cggtctgaac
     6421 cccaacaaca tcggtcaggc gcttcgcgtc gatgccaatg ggtatctcct gaccgcgcaa
     6481 gccggggcca gcaacagccc tgcgaactat acagcgagcg gcagtgcgac caacgatggt
     6541 gacacagtaa gcatcgcggt tcaggacaca gaaacagccc tggtgcagat cctgggcgct
     6601 ttcaccatga ccctgctctt tgaagcctcg cccgacaacg gaacgacatg gtatagcgtc
     6661 tcaggcgtgc agggaaccgg caccgtcgcc agtagcgcca ccgcgccggg ttactggcgc
     6721 gtgaatgtgg caggatttac gaacttccga atcagagcac acccgatcac cagcggcacc
     6781 gcaactatta ccattcgtct gacccaggca gtcaacaacg tgagcatcgc caacgcgcag
     6841 ccgctgggcc agcagaccag cgccaacagt tcgagcgtgg tgattgcctc agatcagacc
     6901 gtgccagtca aaggcggtac cgtcgagatc atcggcaacg gcaccggcgt ggtcaacggc
     6961 gtcaatactg acctgattag tagtacggat gtttcagcgt acaagtggct ttcactgcag
     7021 atccagggta cctacaacgc caccctgagc tttcaggcca gcaatgacaa tgtgaactgg
     7081 gtcgcagtca aactgatcct gtttacctcg tatgtggata acaatacgag cagctcgacc
     7141 accagcaccg ggcagatctg gtgtggcccg gtcacgttcc gctacttccg cgtgcgcgcg
     7201 accagcttca ccagcaatac cagcttagca ggcaccctgg aactctacgc gatggcaccc
     7261 acactgccca tc
//
LOCUS       DASZYN010000237         8977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_970966, whole genome shotgun sequence.
ACCESSION   DASZYN010000237 DASZYN010000000
VERSION     DASZYN010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8977
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970966"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..2301)
                     /locus_tag="VHE33_06050"
     CDS             complement(118..2301)
                     /locus_tag="VHE33_06050"
                     /inference="COORDINATES: protein motif:HMM:NF026221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HVT97050.1"
                     /translation="MLNRRTFVKSGAAAGTLAYWTDPLRATATTRTFGNDSVPADRPA
                     WLKSLRRIGQTNITEHDPADLDVEAWADYWSRAKVGVVYVSVTGIIAYYQTQVPFHRK
                     SKYLAGKDFFGELNTAARKRGLRTFARMSPDLNWGDALQAHPEWFMRDPQGNAIPTAD
                     TPELFQTCMFSAYMTGYMSAIMREINAHYDVDGFYMNGWPMFNLPVCYCNICRKLPPP
                     KTPAYWEKFNQRVMELWRLYDGIAKDKNREAFYFANMGGGIHATPNLVQVGEISQWFQ
                     ADNQGRGRMDDPIWGCTQQGRVCHAIMNGKVAANVTGLWSTGPLRWRESIKSQQESEM
                     WLNETVASGMVPYIHVIGGAKGMGEDRRYEKFGEDYFAWTAKHDAHFTNRRSIASIAV
                     VFGQRTNLFYQPPNDMTMQRNMDGLYSALLEGRFLFDFLHEDQLDLDRLEKYAAVILP
                     NIALLSDAQCAQLRAYVDQGGSLLATFETSLYTDQNVRRTDFGLADVLGIHTAGKVIG
                     RRGNSNPFYARIERPHPILSGFADTNWLPGSEYRQPVAVVENPILTVVPGFVAYPPEL
                     SYPPTPQTTEPAVVLQERGHSRTAYFPGNIEHSLWVAGNTDLSRLLQNTIRWIAGENA
                     PVTIEDPHIENPALIEAFAWETEPGFAVHILNYTNPNAHRGWLRATYPIGEQRVRLLV
                     PSGRTIKKVELLRAEKEIAFRKNGNVIEFTIPSIEDYEVAALTAV"
     gene            2572..3579
                     /locus_tag="VHE33_06055"
     CDS             2572..3579
                     /locus_tag="VHE33_06055"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVT97051.1"
                     /translation="MKIRRSLQWLFVCLALTGSIAAQVALPNDPAMGNISGTVMDSES
                     DVVPGATVVLDGPAAQPSRTTVADDNGGFHFDAVTPGTPYHVTIHADGFVQWTSPEMT
                     LQPGQFLFVSNVKLVFSAAATSVTVYASRDQIATEQVKLEEQQRVLGVIPNFYVAYDS
                     NAAPMTAKLKFSLALRAERDPVTLLGVAMVAGMDQAGNTPAYQQGAAGYGQRLGANYA
                     TGFTDIMIGGAILPSVLHQDPRYFYQGTGTTRSRLLHALSSPFICRGDNGKPEPNFSS
                     VGGDLAAGAIANSYYPQSDRGPGLVFGSAAIVTGGRMANAVVQEFVLRNLTPGARNRG
                     R"
     gene            complement(3625..3927)
                     /locus_tag="VHE33_06060"
     CDS             complement(3625..3927)
                     /locus_tag="VHE33_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97052.1"
                     /translation="MRPVQSLAGQTAIVIQRNGNRKEVRFGRDVRLRREGARPPGTTA
                     VLQPVVEGHVDANDTAFDPEDQQLILSDGSVFRITFSYQNFFFAVEPLSARVHLRT"
     gene            complement(5085..5504)
                     /locus_tag="VHE33_06065"
     CDS             complement(5085..5504)
                     /locus_tag="VHE33_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97053.1"
                     /translation="MTRTAAILRSVAAPLIGYAVIVAGTTVSFKLAGGINLRSPLPNF
                     VLGTLGIAVSGLLGGMTAGWVGGRKPVWHATSVLIFLVMDSVTVLFIRHRHRLDPLWF
                     GVLSALGLMLATIVGGILIRSLHRPPRPGCSRPGCSP"
     gene            complement(5523..6365)
                     /locus_tag="VHE33_06070"
     CDS             complement(5523..6365)
                     /locus_tag="VHE33_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVT97054.1"
                     /translation="MSSINVGRLDPLPPLAPFRIGETFIPASAVPLPPHRHRSAQIHF
                     IVEGTYAETSRGKTYELGPGSALFRPAGELHSNCFAGAVHGLLIDLTPAVACRLMPRL
                     NLSEPSWFPAPAFDDLVRSFFYVERENPSERHTLLQALALTLAARISRHARGGGASIP
                     DWIESVVAIIRNRYAEDLRLTGLADEIGVPPRRLAAVFRRCLSKPVGEFLLEVRLHHA
                     HAAVLEGQASLPEIAVSCGFYDQAHMTRAFRRLYGLPPGQLRRRSRSNVQILAGTYKT
                     SRCR"
     gene            complement(6445..7851)
                     /gene="fumC"
                     /locus_tag="VHE33_06075"
     CDS             complement(6445..7851)
                     /gene="fumC"
                     /locus_tag="VHE33_06075"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HVT97055.1"
                     /translation="MSQTEFRIEKDSLGDVRVPAGKLWGAQTQRSLEHFSIGVELMPR
                     EMIAAYAILKKASAHANQAAGWLDQERLSLIDRACDEILAGQHADMFPLHVWMTGSGT
                     QFNMNVNEVISNRCCQFAGTPLGSKTPVHPNDHVNMSQSSNDNFPSAMNIAVAVNVRQ
                     RLIPAVSQLRDAIAKKSEEWHDIIKIGRTHMQDATPLTLGQEWSGYAVMLSDNLVRID
                     EALKGVYELALGGTAVGTGINAAPGFAESAAAEIARLTHLPFVSAPNKFAVQGAHDAL
                     VQFSGTLRTLAVSLYKIANDIRLLSCGPRAGFYELLIPENEPGSSIMPGKVNPTQCEA
                     LTMIAVQVMANDVAAGFAGAGGYLEMNVYKPLMIFGLAQSITLLADGCSNFRRHLVEG
                     ARPNRKKIEEDLARSLMLVTALSPVIGYDKASKIAHHAMEHDLTLKDAALQLGYISEE
                     EFDRIVDPAKMVHPHSAK"
BASE COUNT         1638 a         2315 c         3144 g         1880 t
ORIGIN      
        1 ggaaggcgtg ggctgcttgc tgtggggtga acctggggcg ggcgcagctc tgaccctgct
       61 gagaaagcgg cttgcgaatt ctgcaaaaca tcttccgatc gagttggaac gacgttgtca
      121 cactgccgtg agcgccgcga cttcgtagtc ttcgatcgag gggatggtga attcaatcac
      181 gttgccgttt ttcctaaatg cgatttcctt ttcggcccgc agcaactcga cctttttgat
      241 cgtgcggccg gagggcacca ataggcgcac gcgctgttcg ccgatgggat aggtagcgcg
      301 cagccatccg cggtgggcgt tgggattcgt gtaattgagg atgtgcacgg cgaagcctgg
      361 ttcggtttcc caggcgaagg cttcgatgag cgccgggttt tcgatatgcg ggtcttcgat
      421 cgtgaccgga gcattttctc ctgcgatcca gcgaatcgtg ttctgaagca ggcggctgag
      481 gtcggtgttg cccgcaaccc acaggctgtg ttcgatgttg cctggaaaat acgcggtgcg
      541 gctgtggccg cgctcctgca ggacgacggc gggttcggtg gtttgcggag tgggcggata
      601 agagagctcc ggcggatagg cgacgaagcc ggggacgacg gtgaggatcg gattttcaac
      661 aacagcaaca ggttgacgat attccgagcc aggcagccag ttggtgtcgg cgaatccgga
      721 gaggatgggg tggggccgct cgatgcgcgc gtaaaacgga ttcgagttgc cgcgacggcc
      781 gatgactttt ccggcggtgt gaatgccgag cacatcggcg agtccgaagt ccgtacgtcg
      841 gacgttttga tcggtgtaga ggctggtttc gaaggtggcg agcagcgatc cgccctggtc
      901 gacgtaagct cgtagctgtg cacactgggc atcgctgagg agggcaatgt tgggcaggat
      961 gacggcggcg tatttttcga ggcggtcgag gtcgagctga tcctcgtgga ggaagtcgaa
     1021 caggaatcgg ccttcgagca gcgcggagta gaggccgtcc atgttgcgct gcatggtcat
     1081 gtcgttcggc ggctggtaga agagattggt gcgctggccg aagacgacgg caatgctggc
     1141 gatggagcgg cgattggtga agtgggcgtc gtgcttcgcc gtccaggcga aatagtcttc
     1201 gccgaatttt tcgtagcggc ggtcttcgcc cattcctttg gcgccgccga tgacgtggat
     1261 gtagggaacc atgccgctgg cgacggtttc gttgagccac atttcgcttt cttgctgtga
     1321 cttgatgctc tcgcgccagc gcaggggtcc ggtggaccac aagccggtga cattggcggc
     1381 cactttgccg ttcatgatgg cgtgacagac acggccctgc tgggtgcagc cccagatggg
     1441 gtcgtccatg cggccgcggc cctggttgtc ggcctggaac cactgcgata tttcgccgac
     1501 ctgaacgaga ttgggcgtag cgtgaatgcc gccgcccata ttggcgaagt agaacgcctc
     1561 acggtttttg tctttagcta tgccatcgta gagacgccac aactccatga cgcgctggtt
     1621 aaatttttcc cagtaagcgg gagttttcgg aggcgggagt ttgcggcaga tgttgcagta
     1681 gcagacgggc aggttgaaca tgggccagcc gttcatgtag aagccgtcga cgtcgtagtg
     1741 ggcgttgatt tcgcgcatga tggcgctcat gtagccggtc atgtaggcgc tgaacatgca
     1801 ggtctggaag agttcgggcg tgtcggcggt gggaatggcg ttgccctgcg ggtcgcgcat
     1861 gaaccattcc gggtgggcct ggagcgcgtc accccagttg aggtccgggc tcatgcgggc
     1921 gaaggtgcgc aggccgcgtt tgcgtgctgc tgtgtttagt tcgccgaaga aatctttgcc
     1981 cgcgagatat ttgcttttgc ggtgaaacgg cacctgggtt tggtagtacg cgatgattcc
     2041 ggtgacgctg acatagacga cgccgacttt agcgcgcgac cagtagtcgg cccaggcttc
     2101 gacatcgagg tcggcggggt cgtgctcggt gatattggtt tggccaatgc ggcgcaggga
     2161 tttaagccag gctggccggt ccgcgggcac ggagtcgttg ccgaaagttc gcgttgttgc
     2221 tgtggcgcgg aggggatcgg tccagtacgc cagggtgccg gcagcggcac cggatttcac
     2281 aaaagttctg cgattcagca ttcgaacatt ctccgatacg ggctgcgtaa agggggaagg
     2341 acgcgcgccg gagaatttta agggaatcgg agcgttgatg cggaaggaga taggacgagg
     2401 attctgaaaa atctacacgc atgtatattt tcgaagtttg aaaagcctta ctgcgactgc
     2461 gaaagatgaa tccgcgattg ggttggggca tcagatacgc agttgttttc cccccttcgc
     2521 ctgatcgcgg aggctggtgg aactcgcaaa tactaccagg agttctccgc gatgaagatc
     2581 agacgatcgc tgcagtggct cttcgtgtgt ctggcgttga ccggttcaat agcggcgcag
     2641 gtggcgctgc ccaacgaccc ggcgatgggc aacatcagcg gcacggtgat ggattcggaa
     2701 agcgacgttg tgccgggcgc tacagttgtt ctggatggac cggcggcgca gccctcacgg
     2761 acgaccgtcg ccgatgacaa cggcggattt cactttgatg ctgtgactcc gggaactccc
     2821 tatcacgtga caatccacgc agacggcttt gtgcagtgga cctctccgga gatgacgctg
     2881 cagccggggc agtttttatt tgtttcgaac gtcaagctcg tgttctccgc ggcggcgaca
     2941 tcggtgacgg tgtacgcatc gcgggatcaa atcgcgaccg aacaggtgaa gctcgaagaa
     3001 cagcagcgag tgctgggggt catacccaac ttttatgttg cttacgattc gaacgcggcc
     3061 ccgatgacgg cgaagctgaa gttttctctc gcgctgcggg cagagagaga tccggtgaca
     3121 ttgctcggcg tggcgatggt ggcgggaatg gatcaggcag gaaatacgcc tgcgtatcag
     3181 cagggggcag ccggctacgg gcaacggcta ggagcaaatt atgcaacggg attcacggac
     3241 atcatgattg gcggtgcgat tttgccgtcg gtgctgcatc aggatccgcg ctatttttat
     3301 cagggcacgg gcacgacgag gtcgcggctg ctgcatgcac tttcgagccc gttcatttgc
     3361 cgcggggaca acggaaaacc agagccgaat ttttcgagcg tgggcggcga cctggctgcg
     3421 ggggcgattg cgaacagcta ctatccacaa agcgatcgcg ggcccgggct ggtgtttgga
     3481 agcgcggcca ttgtgacagg tggacggatg gcgaacgcag tggtacagga atttgtgctg
     3541 cggaatctga cgccaggcgc gagaaatcgc ggccgctgac ggcgcaggag acggacgcgt
     3601 caggcgggtt tgtctcggtg cgtttcaggt gcgcaggtgg acgcgggcgg acagcggctc
     3661 aacagcaaag aagaaattct gatagctgaa ggtgatgcgg aagacgctgc cgtcgctgag
     3721 gatgagctgc tggtcctcag gatcgaaagc ggtgtcgttc gcgtcgacgt ggccctcgac
     3781 gaccggctgc aggacggcgg ttgttcctgg aggacgcgcg ccttcacggc ggagccggac
     3841 atcgcggccg aagcggacct ctttgcggtt gccattgcgc tgaatgacga tggcggtttg
     3901 gccggcgagg ctctgaaccg gcctcatctc ccactctgcc cgatacgttt ctgctgcgtc
     3961 tgccatgccg ggaagagtag cccggttttt caacgggtcg aaatccctcg tgggcgtcag
     4021 cggaagtggc ctgcctgtgc cgaatggtgc gcaccaacgg gttatgactc cagtcttgaa
     4081 ccgaaatggt tccgggcgag ggtgggcggt gcaggctgga acgcccttgg ggtaaggagt
     4141 gttctttcca aactggtacg ggcatgaacc tgactggtgg tcgaagagaa ttggatatcc
     4201 cattcccaaa atggatcggg gcgtgacttc ggacaggtgg cggggtcgct tttgtgttga
     4261 tttctggctt agaaaggctt ccgctgcttg ctgtggggtg aacctggcgg gggcaactcg
     4321 gggggccgtg ggttgagtca ttcccaaaat ggatctgggg tgacttcgga cctctggcgg
     4381 agtcgttttt gtgttgattt ctggctcgcg aagtcttcct tgctgtgggg tgaacctggc
     4441 gggggcctga tcttgggatt gggggccaat tgcggcgagt cgttcccaaa atgggtcggg
     4501 gcggcacttc ggtcaggggc cggggtcgtt tttgtgttga tttctggctc gcgaaggctt
     4561 ccgctacttg ctgtggggcg aacctggcgg gggcccaatt tgagggggcg ggagccaatg
     4621 cggcgagtcg ttcccaaaat gggtcggggc ggtacttcgg tcagatggcg gagtcgtttt
     4681 agtgttgatt tctggctcgc gaaggcttcc gctgcttgct gtggggtgaa cctggccgca
     4741 gcctgatctt gggggcggga gccaatgcgg cgaggtcgtt cccaaaatgg gttggggcgg
     4801 tacttcggtc aggagacgag gtcgtttttg tgttgatttc tggctcgcga aggcttccgc
     4861 tgcttgctgt ggggtgaacc tggccgtttc tgctcggggg gtttcgcgct gaatggattg
     4921 cgatgcaagg cctggtttcc gacgcgtggt ttccatcaag gcgtcgtctc catcaagcga
     4981 ggcgtggtct ccaaggcgtc gtctccatga tgcgaggcgt ggtcttcatt cgtgaactga
     5041 gtcttgccgg gtctgcaagg gagagtgcgg gcccacctat ggcgtcaggg tgaacatcct
     5101 ggacgcgaac aaccgggacg cggggggcga tgcagagacc ggatcaggat gcctccgacg
     5161 atggtcgcca gcatgagccc gagggcgctc aggactccga accagagcgg gtcgaggcgg
     5221 tgccgatggc gaatgaagag aacggtcact gaatccatca cgaggaagat caggacgctg
     5281 gtggcgtgcc acaccggttt gcgaccaccc acccaaccag ccgtcattcc gccgagcagg
     5341 ccggagacgg cgatgcccag ggttcccaga acaaaatttg gaagcgggga tcgcaggttg
     5401 attccgccgg ccagtttgaa gctgaccgtg gtgcccgcga cgatgacggc gtacccgatg
     5461 agcggagccg cgacactgcg caatatggct gctgttctgg tcatacccgg cagcagattg
     5521 catcatcggc aacgcgaagt cttgtacgtt cctgcgagga tttgtacgtt tgaccgcgat
     5581 cgccggcgga gctgacccgg gggcaaaccg tagaggcggc ggaacgcgcg ggtcatgtgg
     5641 gcctggtcgt agaatccgca ggaaaccgca atttcaggca gagacgcctg cccttcaaga
     5701 actgcggcgt gcgcgtgatg caggcgcact tccagcagaa actctcccac tggttttgag
     5761 aggcagcggc ggaagacggc cgccagacgc cggggcggga cgccgatttc gtccgcgagg
     5821 ccggtaagac gaaggtcttc agcgtaacga ttccgaatga tggcgacgac ggattcgatc
     5881 cagtccggga tggacgcccc acccccgcgc gcgtggcgcg agatacgggc cgcgagcgtc
     5941 agcgccaggg cctggagaag ggtatgacgc tcgctgggat tctctctttc aacataaaaa
     6001 aacgagcgga caagatcatc gaaggcaggt gcggggaacc aggaaggctc cgacaggttc
     6061 agccggggca tcaggcggca agccacggca ggggtgaggt cgatcagcaa tccgtgcacc
     6121 gcccctgcga agcagttgct gtgaagttct cctgccggcc gaaacagagc cgatcccggt
     6181 ccaagctcat aggtctttcc gcggctggtc tccgcatagg tgccttcgac gatgaagtgg
     6241 atctgcgcgg agcgatgccg atgggggggc agaggcacgg cggaggcagg aataaaagtt
     6301 tcgccgatgc ggaagggcgc cagtggcggc aacgggtcga ggcggccaac attgatgctg
     6361 gacatgggca gacattctac gcggaccggg cagaaagcag acgagccgaa ccgggccgat
     6421 ggtgatgcga ggccggcggt ggagctactt tgcagaatga ggatggacca tttttgccgg
     6481 gtcgacgata cggtcgaact cttcttcgct gatgtagccg agctggaggg cggcgtcctt
     6541 cagggtaagg tcgtgttcca tggcgtggtg ggcgatcttt gaggccttgt cgtagccgat
     6601 gacgggagaa agagccgtga ccagcatgag ggagcgagca aggtcttctt cgattttctt
     6661 ccggttgggc cgggcgcctt cgacgagatg cctgcggaaa ttgctgcagc catcagcaag
     6721 gagcgtaatg gactgcgcca ggccgaagat catcagcggc ttgtagacgt tcatttccag
     6781 atagccgccg gctccggcga aaccggcagc gacgtcgttg gccatgacct gaacggcgat
     6841 catggtgagc gcttcgcact gtgtggggtt gaccttgccg ggcatgatcg aggagccggg
     6901 ctcgttttcg gggatgagga gttcgtaaaa accggcgcgg gggccgcagg agaggaggcg
     6961 aatgtcgttg gcgattttgt aaagcgagac cgcgagggtg cgcagggtgc cggagaactg
     7021 aacgagggca tcgtgcgctc cctgcactgc gaatttattg ggagcggaga cgaaggggag
     7081 atgcgtgagc cgcgcgattt ccgccgccgc ggattcggcg aagcctggag cggcgttgat
     7141 gccggtaccg acggcggttc cgccgagagc gagctcgtag acgcccttca gcgcttcatc
     7201 gatgcgaacg agattgtcgc tgagcatgac cgcgtagccg gaccactcct ggccgagggt
     7261 gagcggcgtt gcgtcctgca tatgggtgcg gccgattttg atgatgtcgt gccactcttc
     7321 ggattttttc gcgatcgcat cgcgcagctg tgagacggcg gggatgaggc gctgccgcac
     7381 gttgacggcg acggcgatgt tcattgcgga ggggaagttg tcgtttgagg actgcgacat
     7441 gttgacgtgg tcgttgggat ggaccggcgt tttgctgccg agcggcgtgc cggcgaactg
     7501 gcagcagcgg ttggagatga cctcgttgac gttcatgttg aactgcgtgc cgctgccggt
     7561 catccagacg tggaggggaa acatgtcggc atgctggccg gcgaggatct cgtcgcaggc
     7621 gcgatcgata agggagaggc gttcctggtc gagccagccg gcggcctggt ttgcatgagc
     7681 ggaagctttt ttgaggatgg cgtaggcggc gatcatttcg cgcggcatga gctcgacgcc
     7741 gatgctgaag tgctcgaggg agcgctgggt ttgcgcgccc cagagtttgc cggcggggac
     7801 gcgcacatcg ccgagtgaat ctttttcgat gcggaattcg gtttggctca tgaggggtct
     7861 gtcctgctct gcgaatggga tgccgggcgc agagatggaa tgtccatgaa gatcattgaa
     7921 caccctggcg gagcaagcgg cgcggcgatt taccgctggc gtggtgtcgc tgctttgcga
     7981 atttcgtcca tgcgggctgg gcggtgaggt ggcagcgaaa tgcaggtagg gccgaccgac
     8041 ccgatacgaa gggggcggtt tctccgattc gagccagaac cctgcgaatg ggcgcagccc
     8101 aagccgcgtc catggggcgt tcgtcttctt tcaggccacc ccggcgaaac ttcgtttcgc
     8161 caggaccccg gcctcccacc ctcgcgccct ggcatttcct cgctcgcgag gaaacgccac
     8221 cgggttttga agctgaagtg ggattatttt cggagccttt tggcaggacc gaacagtttg
     8281 cggggaaagt gcttcttccg gcgaaaatct tttgtcagcg gctaaagccg gacagattct
     8341 gcagcgctca gtggcccgac tgaagccggt ctcttgcaaa gcgctggttc ttgcgcaaat
     8401 tgtgaagtga catcgcgccc tgacacgttc atcgatccgc cgattccggg ccggctccca
     8461 ccgcggtaca cattcagcag caagcctcac acggttggag caccctaacc cgaaaggtgc
     8521 tcagcgaatt tcgggattga atcgcaggac gtcaagggat cgatttacag gccaccccgg
     8581 cgaaacgttg tttcgtcggg accccggcct cccaccctcg tgccctggca tttcctcgct
     8641 cgcgaggaca tgccaccggg ttttggggtg gagccccact gcaggaagaa gcctcagcgg
     8701 ctgaatccgc gtatttgttg gggtcgaagg tgcgcgacat gactgtcgcg cctacctcgt
     8761 ggcgttgcgc ctgccttgtg gagttgcgcg tgccttgtgg agttgcgcct gccttgtgga
     8821 gttgcgcgtg cctcgtggtg ttccatgttg caaacaccga gctgaggcga agcaggttcc
     8881 tcggctcacc accccaaact gaaatgcgtt tggggcccct ttcgctcgga atgacaatgt
     8941 tatttttgta gacggttttt cgtgggggct ggtgttt
//
LOCUS       DASZYN010000238         2745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1163592, whole genome shotgun sequence.
ACCESSION   DASZYN010000238 DASZYN010000000
VERSION     DASZYN010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163592"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="VHE33_06080"
     CDS             <1..1212
                     /locus_tag="VHE33_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HVT97056.1"
                     /translation="SLPAPIAALPSFTGKVAPISNDERRVRIERAKQLMAENKLDAIV
                     LANNSTSSMYFANIGLNGGERLWALVIPAKAKPFVVCPAFENVRAHELLAAGPFGTDA
                     DVLTWQEDESPFALTVKGLADRGIATGRVGLDEEMKFVFSNGLALAGPHLTFVIATPV
                     TAGCRMIKDAHEMECLRTASAATLAVYKAVYQSLREGMTTIDVHGLIDAAYRKTGLRG
                     EASLNIGTFTASPHGSRQVQTIREGTVLMVDDGCTVEGYQSDITRTFVLGKPTEKMKQ
                     VFDIVHQAQQAALKQARPGVELASVDAAARKVIVDAGYGPGFKYFSHRVGHGLGMDGH
                     EWPYLVKNDMFGWNLAPHMRAGMVFSDEPGIYIPGEFGIRLEDDMHVTESGAELLTPA
                     SPSLEDPFGSA"
     gene            complement(1223..>2745)
                     /locus_tag="VHE33_06085"
     CDS             complement(1223..>2745)
                     /locus_tag="VHE33_06085"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HVT97057.1"
                     /translation="ITGLLRDDFTPVVKTTDWPLGHIIVGRVGGTEYPVANPDDSRNI
                     LTVRDTPDGPRQTIPRSAWSFTAPTQTAKNGGIHLGGFHGVDGFQPGRIYELVYVVKD
                     PVVAGLGFAAVRDFVSWVKFDTAAVTHVDRAYAAGISQCGRFLRDFLYEGFNQDESGR
                     MSLDGILAHVGGGGRGSFNYRFAQPSRDSQPMSSIFWPTDIFPFTDEPEQDPAHPQAP
                     AQGLLDRTDAEHAMPKIFLSHTSYEYWGRAASLMTTTADGKQDVPIHPNVRVYFFTGL
                     QHFSGPWPPARGTGEIASQNPESPLPIRWFWRAMITNMNAWVRDGVAPPPSRYPHVAD
                     GTLVPREKLAFPAIRGVHPPNNYNEAFHLDFGPNWRQTRILTVQPPTVGAPFPVLVPQ
                     VDADGTDRAGIHLPEIAVPLATYTGWNLRDPATGASDERVSFLGSWFPFAKTVAARRA
                     DGDPRPSIEERYSSREDYLIRYNKAVDNLVHERWILPEDAASLKQQGASEWDYATR"
BASE COUNT          548 a          755 c          933 g          509 t
ORIGIN      
        1 tcccttcccg ctccgattgc ggcgttgccc tctttcaccg gaaaagttgc acccatttcg
       61 aacgacgagc gccgtgtgcg aatcgagcgc gcgaaacagc taatggcaga aaacaagctt
      121 gatgccatcg tgcttgcgaa caactcgacc agctcaatgt acttcgccaa tatcggcctg
      181 aacggcggcg agcggctgtg ggcgctggtg attccggcca aagcaaagcc ctttgttgta
      241 tgcccggcgt ttgaaaatgt gcgagcgcac gagttgctcg ctgcaggacc attcggcacc
      301 gacgccgatg tgctgacgtg gcaggaggat gagagcccgt ttgccctgac ggtgaaagga
      361 ctggccgatc gcggcatcgc gacgggacgt gtgggcctgg atgaggagat gaaattcgtc
      421 ttctcgaacg gcctggcgct cgccgggcca cacctgacgt ttgtgattgc aacgccggtc
      481 actgccgggt gtcgcatgat caaggacgcg cacgagatgg agtgcctgcg cacggcaagc
      541 gccgcgacgc tggccgtcta caaggcggtg tatcaatcgc tgcgtgaggg catgacgacg
      601 atcgatgtgc atggtctgat cgacgcggcg tacaggaaga ccggactgcg cggcgaggcg
      661 agcctgaaca tcgggacgtt tactgcgtcg cctcacgggt cgcggcaggt gcagacgatt
      721 cgcgaaggca cggtgctgat ggttgatgac ggatgcaccg tcgaaggcta tcaatcggac
      781 atcacgcgga cgtttgtgct gggcaagccg accgagaaga tgaagcaggt attcgatatc
      841 gttcaccagg cgcagcaggc ggcactgaag caagccaggc cgggggtgga attagccagc
      901 gtagatgcgg ctgcccggaa ggtaatcgtc gacgcgggct acggccccgg cttcaaatac
      961 ttctcgcatc gcgtgggaca cgggctgggc atggacgggc acgagtggcc ttatttggtg
     1021 aagaacgata tgttcgggtg gaatctcgct ccccacatgc gggcggggat ggttttcagc
     1081 gatgagccgg gaatctacat tccgggcgag ttcggaattc ggctcgagga tgacatgcat
     1141 gtgacggaaa gtggcgccga gttgctgacg ccggccagtc cttcgctgga agatccattc
     1201 gggagcgcgt agagacgaaa atctacctcg tcgcgtagtc ccactcgctc gcgccttgct
     1261 gtttgaggct tgcagcgtct tcgggcaaaa tccagcgctc gtgcacgagg ttatcgaccg
     1321 ctttgttgta gcggatgaga tagtcctcgc ggctggagta gcgctcttcg atggaggggc
     1381 gcgggtcgcc gtcggctctg cgcgccgcga cggttttcgc aaacggaaac caggagccca
     1441 ggaaagagac ccgttcgtcc gatgcgcctg tggctgggtc gcgcagattc catcctgtgt
     1501 acgtagcgag cggcacggcg atctccggca gatggattcc agccctgtcg gtgccgtcag
     1561 cgtcgacctg cggaacgagc acgggaaatg gagcaccaac ggtagggggc tgaaccgtca
     1621 aaatccgcgt ctgtcgccag ttggggccga agtcgaggtg gaatgcctcg ttgtaattgt
     1681 tgggcggatg tactccacga attgccggaa atgccagctt ctcgcgtggc acgagggtac
     1741 cgtcagcaac atgcggatag cggctcggag gcggtgcaac gccatcgcga acccaggcat
     1801 tcatgttggt gatcatggct cgccagaacc agcggattgg caatgggctt tcaggattct
     1861 ggctggcaat ttcgccggtg ccgcgggccg gcggccaggg tccggaaaaa tgctggaggc
     1921 cggtgaagaa gtagacgcgg acgttgggat ggatcggcac atcctgcttg ccgtcggcgg
     1981 tcgtggtcat cagcgatgcg gcgcgacccc agtattcgta ggaagtgtgc gagagaaaga
     2041 tcttcggcat cgcgtgctcg gcgtcggtgc gatcgagcag cccctgggcc ggagcctgcg
     2101 gatgggcagg gtcctgttcc ggctcgtcgg taaaaggaaa aatgtcggtc ggccagaaga
     2161 tggacgacat cggctgggaa tcccgcgatg gctgggcgaa gcgatagtta aagctgccgc
     2221 ggcccccgcc gcccacgtgg gcaagaatgc cgtcaagaga catccgcccg gattcatcct
     2281 gattgaagcc ttcatagaga aagtcgcgga ggaaccgccc acattgcgag atcccggcgg
     2341 catacgcgcg atcgacatgg gtaactgctg cggtatcaaa cttgacccag gagacgaagt
     2401 cgcgcacggc ggcgaagccg agccccgcga ctaccggatc cttcaccaca tagacgagtt
     2461 cgtagatgcg gcctggctga aagccgtcga ccccgtggaa tccaccaaga tggataccgc
     2521 cattctttgc agtctgggtc ggcgcagtaa aggaccaggc ggagcgcggg atggtctggc
     2581 gcggcccgtc cggagtgtca cggacggtga ggatgtttcg cgagtcgtcg ggattcgcaa
     2641 ccggatattc cgtgccgccg actcggccca cgatgatgtg gcccagcggc cagtcggtgg
     2701 ttttcacgac aggggtgaag tcatctcgca gaagaccggt aatcg
//
LOCUS       DASZYN010000239         3739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1230401, whole genome shotgun sequence.
ACCESSION   DASZYN010000239 DASZYN010000000
VERSION     DASZYN010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230401"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VHE33_06090"
     CDS             <1..411
                     /locus_tag="VHE33_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HVT97058.1"
                     /translation="DRWGGVLDELLGLVDIFLPNEDEACRITRSASAELAIRQLSERV
                     PLVAVKCGRRGALVQQGQQLWQVSGEPVSPVDTIGAGDSFDAGFLSAYLAGKTPEQCA
                     AFGNRTAALSTQRAGGTESFRDRALVRDFLGRTA"
     gene            complement(474..2774)
                     /locus_tag="VHE33_06095"
     CDS             complement(474..2774)
                     /locus_tag="VHE33_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97059.1"
                     /translation="MKGKMAALRCSILAAGVLVLSLPAASQTKWEIIGPGGGGTTIGP
                     TISPHDPAVVVEHCDMTGGYVTQDNGRSWHMFNLRGGMNVFAFDPVQPHVIYAGNAGL
                     WRSTNAGQNWSLVFPDPRRNTVEHQVGDHADYSLTSDDPTYPGGEISAIALSPAQGSE
                     TKPRIYVAFATGKQSSVVLASEDDGTTWRRLTTIPQHVLLLSAQQDRVTAIAGNAAYQ
                     IEPSGRIEELGHIPVPFHAAGATRSSQTTSFYATGTDGLVYSSRDGGRHWDSITPQLH
                     QSAGRFEAIAATDANPNVAYVGFRRLQIGEGAQNLFNGIAKTTDGGRSWTIVFRESNR
                     PASNLKGTWIEQRARQGNDTIWFDTPYSLAVAPSDPKQVYATDLFRTYRTLDGGVSWK
                     EVNSVEKNGGWSSPGLDVTTNYGVQFDPFDSHHIYIDSTDIGLFQSRDGGRTWIGSSE
                     GVPDNWRNTTYWLAFDPTQRGLMWGAFSGTHDLPRQKMWRNRSPQTFTGGVAVSTDGG
                     MHWQPSASGLPQDSITHILLDPNSPPGKRTLYVCAFGHGVYKSVDSGKTWARKSEGIE
                     GSEPFAWRITRDNHGTLYLVVARRSDKPGAGPGAAGALYRSIDAAEHWQRVALPEGVT
                     GPTALEIDPRDSNRLYLTAWGREGISADHDGGVFVSEDAGKTWKNIFSEGQHVYDLTI
                     DPSHPDTLYITGFDAAAYRSVDRGAHWARIQGYDFKWGHRVTVDPNDPTQIYINTYGG
                     GVWHGPAKGDPNQKETILNPVPVAHE"
     gene            2922..>3739
                     /locus_tag="VHE33_06100"
     CDS             2922..>3739
                     /locus_tag="VHE33_06100"
                     /inference="COORDINATES: protein motif:HMM:NF019625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HVT97060.1"
                     /translation="MILQQLAEARRQGRRMGIYSVCSAHDWVLEAACDQAVEDGSHLL
                     IEATSNQVNHRGGYTGMVPRRFRDRVAEIATSRGLDLSRLILGGDHLGPNPWQHLPAE
                     RAMREAEETVRGYVGAGFQKIHLDASMSCLGDPVPLPVETIARRAAALCAAAEAEAGA
                     VRPVYVIGTEVPTPGGATESLKELQVTSREAAAETVAIHQRVFAEAGLESAWTRVIAL
                     VVQPGVEFNHTSVVDFDPSRAGHLPALLDELKTLVYEAHSTDYQRPEAYRELVR"
BASE COUNT          682 a         1035 c         1253 g          769 t
ORIGIN      
        1 gatcgctggg gcggagtgct cgacgaactg ctggggctgg tggacatctt tcttcccaac
       61 gaggacgagg cgtgccggat cacgcggagt gcatccgcag agctggcgat tcgtcaactg
      121 tcggaacggg tgccgctggt ggcggtgaag tgcggcaggc gcggagctct tgtacagcag
      181 ggacaacagc tctggcaggt cagcggcgag ccggtttcgc cggtcgacac catcggagcc
      241 ggcgacagct tcgatgccgg tttcttgtcg gcgtacctcg cgggaaagac gccggagcaa
      301 tgcgcggcgt tcggcaaccg cacagcggcg ctatccacgc aacgggccgg cggtacggaa
      361 tcgtttcgcg accgagcgct ggttcgggat tttctgggcc gcacggctta atcgcggccg
      421 aggcatctgc ttttgagttc gggcggagga cgcgcggaac ccgcgcaggc tccttactcg
      481 tgcgccaccg gaactggatt cagaatcgtt tccttctggt ttggatcgcc tttggcgggt
      541 ccgtgccaaa cgccaccacc ataagtattg atatagatct gggtgggatc gttcggatcc
      601 acggtcacgc ggtgtcccca cttgaagtcg tatccctgaa tacgggccca atgcgctccg
      661 cgatcgacgg atcggtaggc cgcggcgtcg aagccggtga tgtaaagggt gtcgggatgg
      721 ctggggtcga tcgtgaggtc gtagacgtgt tgtccctccg agaagatgtt cttccacgtt
      781 ttcccggcat cttcggagac gaacacacca ccatcgtggt cggcgctgat gccctcgcgt
      841 ccccaggcag tgagatagag ccgattcgag tcgcgagggt cgatttcgag ggcggtcgga
      901 ccggttacac cttcgggaag agcaacgcgc tgccagtgtt cggctgcgtc gatggaacga
      961 tagagcgcgc ctgcggcgcc tggtccggca ccgggcttat cgctgcggcg agcgacaacc
     1021 aaatagagcg tgccatgatt gtcccgcgtg atacgccacg cgaacggctc ggagccttcg
     1081 atgccctcgc tctttcgcgc ccatgtcttt ccgctgtcga ccgacttgta gactccgtga
     1141 ccgaaggcgc acacgtagag ggtgcgcttc ccgggagggc tgttcggatc gaggaggatg
     1201 tgagtgatgg aatcctgagg cagtccgctg gcactcggct gccagtgcat gccgccgtcg
     1261 gtggagacag caacgccgcc ggtgaatgtt tgtggagagc gattgcgcca cattttctgg
     1321 cgaggaaggt cgtgcgttcc gctgaaagcg ccccacatca atccgcgctg agtgggatcg
     1381 aatgcgagcc agtaggtggt attgcgccag ttatcgggaa caccctcaga gctgccaatc
     1441 cacgtgcggc caccatcgcg gctctggaat aaaccgatgt cggtggagtc aatgtagatg
     1501 tggtgcgagt cgaagggatc gaactgcaca ccatagttgg tggtcacatc gagtccaggc
     1561 gaactccagc cgccgttctt ctccaccgaa ttgacttcct tccaggaaac gccgccgtcc
     1621 agggtgcgat aggtgcggaa gagatcggtc gcatacactt gcttcggatc ggacggcgca
     1681 acggccaggc tgtaaggcgt gtcgaaccag atcgtgtcgt ttccctgtcg cgcacgttgc
     1741 tcgatccagg tccccttgag attggaggca gggcgattcg actccctgaa gacgatcgtc
     1801 catgaacgtc caccgtcggt ggtcttcgcg atgccattga agaggttttg cgctccttcg
     1861 ccgatctgaa ggcggcggaa tccgacgtag gcgacgttgg gatttgcgtc ggtggctgcg
     1921 atggcttcga agcgtcccgc gctctgatgc aactgaggag tgatggaatc ccagtgtcgt
     1981 ccgccgtcac ggctggagta gacgaggcca tctgtgcccg ttgcgtaaaa cgaagttgtc
     2041 tggctggatc gagttgcgcc ggccgcgtga aacggcacgg ggatatgccc aagctcctcg
     2101 atacggccgg agggttcaat ctggtaggcc gcattgccgg cgatagctgt gacgcgatcc
     2161 tgttgcgctg aaagaagaag cacatgttgg ggaatggttg tgagacgccg ccaggtggtt
     2221 ccatcgtcct cggaagcgag cacgacagag gattgcttac cggtagcgaa cgcgacataa
     2281 atacgcggct tcgtttccga tccctgggcc ggggagagcg cgattgcgga gatctctccg
     2341 cccggatagg ttgggtcgtc cgaggtcagg ctgtagtcgg catgatcgcc gacctgatgt
     2401 tcgaccgtgt tgcgtcgcgg atcaggaaag accagcgacc agttctgacc ggcattcgtg
     2461 ctgcgccaga ggccggcatt gccggcataa atcacatgcg gctggacggg gtcgaaggcg
     2521 aagacgttca ttcctccgcg aaggttgaac atatgccacg acctgccgtt gtcctgggtg
     2581 acataaccgc cggtcatgtc acagtgttcg acgacaaccg ccggatcgtg gggactgatg
     2641 gtgggaccga ttgtggtgcc gccgccaccg ggcccgatga tttcccattt ggtctgggac
     2701 gctgccggga gagaaagaac caaaactcct gctgcgagga tcgagcagcg cagcgccgcc
     2761 atcttgcctt tcatctgcaa ttctccagtg cgcaattgtg cgagggatcg ctttcgaaga
     2821 tcacggccta ttgttgtttc tattgtgatt catgtacgta tactttgatt gtcaatcatt
     2881 actatttctt ttgattgaac tggagggtag cagagttggc aatgatcctg caacaactgg
     2941 ccgaggcgcg gcgccaggga cgccggatgg gaatctattc ggtgtgttcg gctcacgact
     3001 gggtgctcga ggctgcctgc gatcaggccg tcgaggacgg ctcgcacctc ctgattgagg
     3061 ccacttcgaa tcaggtgaat catcgcggcg gatacacggg catggttccg cggcgatttc
     3121 gggatcgtgt ggccgagata gcgacgagcc gcggtctgga tttgtccagg ttgattctgg
     3181 ggggcgacca tctcggtccg aatccgtggc agcatttgcc tgccgagcgg gcgatgcggg
     3241 aagcggagga gactgtccgc ggctatgtcg gtgcaggctt ccagaaaatt cacctggatg
     3301 ccagcatgtc gtgcctcggg gatcctgtgc cgctgccggt tgagacgatc gcgcggcgcg
     3361 ctgcggcttt gtgcgctgcg gcggaagcgg aggccggcgc tgttcgcccc gtctacgtga
     3421 tcggcacaga ggttccgacg ccaggcggcg caaccgaatc gttgaaggaa ctgcaggtga
     3481 catcgcggga agcggcggcg gagacggtcg cgatccatca gcgcgtcttt gccgaggctg
     3541 gcctcgaatc tgcgtggacg cgtgtgatcg cgctggtggt gcagcccgga gtcgagttca
     3601 atcacaccag tgtggtggat ttcgatccga gtcgcgccgg gcatcttccc gcgctgctcg
     3661 acgaactgaa gacgctggtc tatgaggcgc attcgaccga ctatcaaagg cccgaagcgt
     3721 accgtgagct ggtgcgaga
//
LOCUS       DASZYN010000240         9210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_971161, whole genome shotgun sequence.
ACCESSION   DASZYN010000240 DASZYN010000000
VERSION     DASZYN010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 9210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9210
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971161"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VHE33_06105"
     CDS             <1..670
                     /locus_tag="VHE33_06105"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HVT97061.1"
                     /translation="GVSAEGAALCYRTICVEGARYFGPDAHPEVSMHTLSFARYRRLS
                     ERQDWRGTSELLLTSAKKLAKVGADFLICPANTPHLAFPFFDSRSPLPWLHIADVVAD
                     EALRRGFHRIGILGTRYTMQSSLYADRCNPRDLEALSPNADECATINRFIYDELVRGV
                     CTPAATEYHQQVIARLKSEGCDAVVLGCTEIPLIISDANSPLPTLDSTRLLAHAALRK
                     AAGK"
     gene            784..1974
                     /locus_tag="VHE33_06110"
     CDS             784..1974
                     /locus_tag="VHE33_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97062.1"
                     /translation="MATTTSTWSHPVATARRATIPWYLWTVALAVTSVTIGAHWDVSW
                     HRSIGRDTFWTPAHMAIYLCGILAGIACGYLILRSTFAPTPELDATSVRVFGFRGPLG
                     AFIAAWGGIAMLTSAPFDNWWHNAYGLDVQIVSPPHTLLMLGIFAIEIGAALLILAQM
                     NRDAADGAAPNPGLQWLMMYIFGLMMVLTMFFRMEYTWDVMLHRAGAYISMAIGVPLY
                     FAAMWKASRHRWACTLICTFYSLALVAFILILPLFPATPKLGPVYQAVTQFIPPKFPI
                     LLIVPAIALDLLWRKFGKVKSQSNKILLSLASGPLFVLTLVAAEWPFAAFLMSKASQN
                     RFFGTAYLDYGTPSFSADALRHFVRPQHGLELWTGLLWAMLYAAISVWLGLLLGDWMR
                     KVQR"
     gene            1974..3485
                     /locus_tag="VHE33_06115"
     CDS             1974..3485
                     /locus_tag="VHE33_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97063.1"
                     /translation="MRTRRILPLFLCALTVAPLAHAHVGSPDVYAEGNAGPYKLSIVI
                     RPPLVIPGVAEVEVRSQTPGIHAIAITPLALTGEASRHPPTPDAMKTPSADPSYFTGQ
                     LWIMAPGSWQIRFLVDGDRGHGVWSVPLPAAAQGVRKMQRGLGIFLAALGLLLVLGMT
                     GIIGAAARESQLAPGEFAPPRRRRTAAIAMTVGFAVLVTGVVLGNWWWKSEAASYSEG
                     IYKPLRMDAKLVSGNVLNLTLIDPGWLDAHRTGNFIPDHNHLMHLYMIRWPAIDVVYH
                     LHPDPIASDEFQLALPTIPAGTYHLYADVVHTNGFPDTMVSSVTLPAITARPLAGDDA
                     EGQGSPVFASPETTTTFTLPDGYTMVWNKPASLTARQPNDFTFTLLDRTGHPAQDTTL
                     YMGMLGHAAFVKDDGTVFAHIHPSGTIAMAALMMAEQQNAPASSPAAPNSSSYMKEMP
                     GMDMPETTTGHLPSSVSFPYGFPTPGRYRIIVQMKHSATIETALFDATVSPSA"
     gene            complement(3498..5009)
                     /locus_tag="VHE33_06120"
     CDS             complement(3498..5009)
                     /locus_tag="VHE33_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HVT97064.1"
                     /translation="MARAKSCGLFGFCLLFPVLLHGQVTVANHWQLCSGCGNTSFYHG
                     DFNNDGYEDLAYIESVDRGGYPVFAVEFAKKDGTYLPATEYEVPLNQGDTDSIAQLAV
                     GDFYHDGNLAILAFTYDSGNAYVYRNNGQGALTLQGSFAYAPSGGSVDSASAAVGDYN
                     HDGNLDVAVVNNGYLHIWLGDGKGGFSAGQTQSVNGTTVAMGDFDGDGKADLLIGRDT
                     SATGTAYVYYGDGAGHFPEEVTLSLPQGYAAFTAGDVNSDAKSDVLAVDPAAGANRVF
                     VFYGDPQRQFASRTSIQAGRCVTGDAQVADMDGNGLNDVVVEELDCANPGTGPLWVDV
                     LRRNPDSSYLPDQTVYWAQKSPDGEIHELSQPPLLLRANQDATPDLLVVQCFDSYCSQ
                     TANTTQLNTTSGNWPSCSAPWAAEGITVCSPLNGVSGSLVQFQIGAAGPVPMRDVEVW
                     VDGSKVAEQIDGFSYYTWLRQSVNLGPGSHSVTVFAAGWDQSLVRKSFTLDVQ"
     gene            complement(5265..5903)
                     /locus_tag="VHE33_06125"
     CDS             complement(5265..5903)
                     /locus_tag="VHE33_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HVT97065.1"
                     /translation="MVRLARFLVPGFFLFVASAVPALAQFSNPEPQTTVVHNARALRP
                     PAGARVAIVEFEDLECPDCARANPVLKEAAEKYHIPWVRHDFPLPFHTWSFQAAVDAR
                     WFDTKSRRLGDAFRDAVFAAQPTIRSLDDLRTFAQKFAADHKLKFPFVVDPQGRLTQL
                     VKADTDLGQSVGIQHTPTIWVVTNRRSGTPFVEVIDRSKLFELIEEAQAGTK"
     gene            5967..6560
                     /locus_tag="VHE33_06130"
     CDS             5967..6560
                     /locus_tag="VHE33_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97066.1"
                     /translation="MARTAVKARRLTIASGARHALCPDRPRSWTSKDATAAAQDFFTR
                     PRRSAARELLRELCSDDPFDHRCAAELARLVSRRQQDVLAAYSDLLAEVAATLPMEEW
                     QARGYVLVAAAMNAVTHAQRMRMLPLVRARLSEDRIAVRAMALEAFAYLAAREPGLRD
                     EALEKLEAARHSSEPALRARARLMLPVVMKSELTLPR"
     gene            complement(6674..8761)
                     /locus_tag="VHE33_06135"
     CDS             complement(6674..8761)
                     /locus_tag="VHE33_06135"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HVT97067.1"
                     /translation="MSKAVKYAERAVTIAANGAWSVFEKLNSIRPNPSFTPKWSDKPL
                     LKSWQKEKPPLGWPRTTDSLCPKCVPEIRQQIIDGKLPHEILLNEKVGEIKAQIIERD
                     GKILMVKDCPKHGHFEDVMSIDTAFFKHLEEVFPGRDIRAHNDDKLHNHGTSTVTHGR
                     GSVLTIDLTNRCNMMCDPCFMDANQVGFVHELTWDEIKTMLDNAITIKPKRQMSVQFS
                     GGEPTLSPYFLDAVAYARKVGYNSVQAATNGIEFAKSKELCRAAAEAGLRYAYLQFDG
                     IGNAANSHRKVGNLFDVKLQAIHNLHEAGVDIVPVTTIINGINNEQVGHIIQFALDNP
                     KKINFLSFQPVSFTGRDEAVSEERRLAQRYTLSHMAHDVKNQTGLGEPTRDWFPISFM
                     STFSDWADLVHGPNHEWGQLSCGCHPNCGIGMALMIDKETKEAVPVTAFLNADRLAKD
                     IAKVNDAARGKFLSIFGVSLALLRNYDPRKSPTHFKILDLLQKFDKCFGATGRNYGKV
                     TGDRTMADIEQRRADRWNFLFIAGMWFQDLFNYDFRRTEQCIIPYATQEGEISFCAYN
                     TGIGWRNIIEKMHMTATLTKWYEEHGRHEIFAGGKKVGMESAQHTLILNQEHVDAAAN
                     NSLEKLGIAKNAREEKLRAREAKLKQDAENARMAKLYREQILGEKPVEGLVTIDSIAP
                     AKPVEKAEPVMGD"
BASE COUNT         1655 a         3361 c         2387 g         1807 t
ORIGIN      
        1 tggagtctcc gcggaaggcg ccgccctctg ctatcgcacc atctgtgtcg aaggcgccag
       61 atacttcggg cccgatgcgc accccgaagt ctcaatgcac actctgagct tcgcccgcta
      121 ccgccgcctt tccgagcggc aggactggcg cggcacctct gaattgctcc tcacctccgc
      181 aaagaagctt gcgaaggtcg gcgccgactt cctcatctgc cccgcgaata cgccgcatct
      241 cgctttcccc tttttcgact cgcgctcccc gctgccctgg ctgcacattg ccgacgtggt
      301 tgccgacgaa gctctccgcc gcggcttcca ccgcatcggt atcctcggca cgcgctacac
      361 catgcagagc agcctttatg ccgaccgctg caacccccgc gatcttgaag ctctcagccc
      421 caacgccgat gagtgcgcca cgatcaaccg cttcatctac gacgagctgg tccgcggtgt
      481 gtgcactccg gccgccactg aataccatca gcaagtcatc gcccgcctca aatctgaagg
      541 ctgcgacgcc gtcgttctcg gctgcaccga aatccccctc atcatcagcg acgccaactc
      601 tccgcttccc accctcgact ccaccagact tctcgcccac gccgcactac gcaaagctgc
      661 tggaaaatag tcggctgcac gttcgtcaaa tacagctcga cctgcaacgt ccgtcccgcc
      721 cttgttttgc gtctatccgc tgtacgctat ccgcggtacg ctgaaaggca gaggatcccc
      781 agcatggcca ccacgaccag cacctggtcg catccggttg cgaccgcgcg cagggccacc
      841 atcccctggt atctctggac cgtcgctctt gccgtcacct ccgtcaccat cggcgcgcac
      901 tgggacgtct cctggcaccg ctccatcggc cgcgatacgt tctggacgcc cgcccacatg
      961 gccatctacc tgtgcggcat cctcgccggc atcgcctgcg gctacctcat cctgcgctcc
     1021 accttcgcac caactccgga actcgatgcc acctccgtcc gcgtcttcgg ttttcgcgga
     1081 cccctcggcg ctttcatcgc cgcctggggc ggcatcgcca tgctcacttc tgccccgttc
     1141 gataactggt ggcacaacgc ctacggtctc gacgtccaga tcgtttcccc gccccacacg
     1201 ctgctcatgc tcggcatctt cgccatcgaa atcggcgcgg cgctgctcat cctcgcccag
     1261 atgaatcgcg acgccgccga tggcgccgca ccgaacccgg gcctgcagtg gctcatgatg
     1321 tacatcttcg gactcatgat ggtcctcacc atgttcttcc gcatggaata cacatgggac
     1381 gtcatgctcc accgcgccgg cgcctacatc tccatggcca tcggtgtccc cctctacttc
     1441 gccgcgatgt ggaaagcctc gcgacaccgc tgggcctgca ccctgatctg caccttctac
     1501 tcgctggccc tcgtcgcctt catcctgatc ctcccgctct ttccggccac gccgaaactc
     1561 ggccccgtct accaggcggt cacgcagttc atcccgccga agttccccat ccttctcatc
     1621 gttcccgcga tcgcactcga cctgctgtgg cggaaattcg gcaaggtgaa gagtcagagc
     1681 aacaagattc tgctgtcgct cgccagcggc ccgctcttcg tcctgactct ggtcgccgcc
     1741 gaatggccct ttgccgcctt tctgatgtcg aaagcctccc agaaccggtt cttcggcacg
     1801 gcctaccttg actacggcac gccctcgttc agcgccgatg cgcttcgcca cttcgtccgc
     1861 ccgcagcatg gcctcgaact ctggaccggc ctcctgtggg ccatgctcta cgccgccatc
     1921 tccgtctggc tcggcctcct gctcggcgac tggatgcgca aggtgcaaag gtaatgcgaa
     1981 ctcgccgcat tcttcctctc tttctttgcg cgctgacagt cgctcctctc gcccacgccc
     2041 acgtgggcag ccccgatgtc tacgccgaag gcaacgccgg cccctacaaa ctctccatcg
     2101 tcatccgccc gccgctcgtc atccccggcg ttgcggaagt cgaggtccgt tcccaaaccc
     2161 ccggcattca cgccatcgcc atcacgccgc tcgcgcttac cggcgaagcc tccaggcatc
     2221 cgcccacacc cgacgccatg aagaccccca gcgccgaccc cagctacttc accggccagc
     2281 tctggatcat ggcgcccggc tcctggcaga ttcgctttct cgtcgatggc gaccgcggac
     2341 atggcgtctg gagcgtgcct ctccccgccg ccgcccaggg cgtacgaaaa atgcagcgcg
     2401 gcctcggtat ctttcttgcc gcgctcggcc tgctcctggt cctcggcatg acgggcatta
     2461 tcggcgccgc cgctcgcgaa tcccagctcg cccccggcga attcgcacca cctcgccgtc
     2521 gccgcaccgc cgccatcgcc atgacagtgg gtttcgctgt ccttgtcacg ggcgtcgtcc
     2581 tcggcaactg gtggtggaaa agcgaagctg cctcttactc cgagggaatc tacaagcctc
     2641 tccgcatgga tgcgaagctc gtctccggca acgtcctcaa tctcaccctc atcgatcccg
     2701 gctggctcga cgcccatcgc accggcaact tcatccccga ccacaaccac ctcatgcacc
     2761 tctacatgat tcgctggccc gcaatcgacg tggtctacca tctccatccc gacccgatcg
     2821 ccagcgacga gttccaactc gcgctcccca ccatccccgc cggcacgtat cacctctacg
     2881 ccgatgtcgt gcacaccaac gggtttccag acacgatggt ctccagcgtc acactgcccg
     2941 cgatcactgc gcgcccgctc gccggtgacg atgccgaagg tcagggctct ccggttttcg
     3001 catcgccgga aacaacaacg accttcacgc ttcccgacgg ttacaccatg gtctggaata
     3061 aacccgcctc gctcaccgcg cgccagccca acgacttcac cttcacgctc ctcgaccgca
     3121 ccggccatcc cgcgcaggac accaccctct acatgggcat gctcggtcac gccgccttcg
     3181 tcaaagacga cggcaccgtc ttcgcccaca tccacccgag cggaaccatc gccatggccg
     3241 ccctcatgat ggccgagcag caaaacgcgc ccgcgtcctc accggccgcg cccaattctt
     3301 cttcctacat gaaggagatg ccaggcatgg acatgccgga gacaaccacc ggccatctcc
     3361 ccagctccgt cagctttccc tacggatttc ccactcccgg ccgctatcgc atcatcgtcc
     3421 agatgaagca cagcgccacc atcgaaaccg ccctcttcga cgcgacggtg tcaccatcgg
     3481 catgatgggt cgcccgccta ctgcacatcc agcgtgaacg acttcctgac cagcgactga
     3541 tcccaacccg ccgcaaacac cgtcacgctg tgggatcccg gaccgaggtt cacactctgc
     3601 ctgagccatg tgtagtaaga gaatccgtca atctgctcgg ccaccttgga tccgtccacc
     3661 cacacttcga catcgcgcat cggcaccgga cccgcggctc caatctggaa ttgcacgagc
     3721 gaccccgaca cgccgttcag aggcgaacag accgttatgc cctccgcagc ccatggagct
     3781 gaacaactcg gccagtttcc cgaggtcgtg ttcagctgcg tcgtattggc cgtctggctg
     3841 caatacgaat caaagcactg cacaaccaga aggtccggcg tggcgtcctg attcgcgcgc
     3901 agaaggagcg gcggctggct cagctcatga atctctccat ccggcgattt ctgcgcccag
     3961 tacaccgtct ggtccggcag ataggacgag tcaggatttc tccgcagcac gtcgacccac
     4021 aggggccccg tgcctggatt ggcgcaatcc agctcctcga caaccacatc gtttagtccg
     4081 ttgccgtcca tatccgccac ttgcgcgtcg cccgtcacgc aacgaccggc ctgaatcgag
     4141 gtacggctcg cgaactggcg ctgcggatcg ccataaaaaa caaacacccg gttggcgccg
     4201 gcagcaggat cgacagcgag cacatccgat ttcgcgtcgc tgttcacgtc gcctgccgta
     4261 aacgcggcgt agccctgcgg caggctgagc gtcacctctt caggaaaatg ccccgcgccg
     4321 tcgccgtaat acacataagc ggtgccggtc gcgctcgtat cccgcccaat cagcaaatcg
     4381 gctttgccat cgccgtcgaa atctcccatc gccaccgtgg tgccgttcac gctctgcgtc
     4441 tgccccgcag agaacccgcc ctttccatcg cccaaccaga tgtgcaaata gccgttgttc
     4501 accactgcca cgtcgaggtt gccgtcgtgg ttgtagtcgc caactgcggc agacgcgctg
     4561 tccacgctgc ccccggaggg ggcgtaagcg aacgatccct gcagcgtgag cgcgccttgc
     4621 ccgttgtttc ggtagacgta agcgttcccg ctgtcatagg taaacgccag aatcgccaga
     4681 ttcccatcgt gatagaagtc cccgaccgcc aactgcgcga tcgagtcggt gtctccctgg
     4741 ttgaggggga cctcgtactc ggtcgccggc aagtaggtgc cgtccttctt cgcgaactcg
     4801 actgcgaata ccggataccc tcccctgtcg acgctctcga tataggcgag atcctcgtag
     4861 ccgtcgttat tgaaatcgcc gtgataaaag gacgtgttgc cgcaaccgga gcacagttgc
     4921 cagtggttag cgactgtaac ctgcccatgc agaagaacgg ggaacagcag acaaaagcca
     4981 aaaagcccac acgactttgc gcgagccatt agaaacctcc gaaagacagc tgaattgttt
     5041 cgaacgggac gtgcgggcca gacaatgtac gcctgtagga tgcgttgggt tcgcatcatc
     5101 ggttgtccag aatgtctgca gagtccccag cttgacggac gaccaggccc ctgatttccc
     5161 gttgccccga cgccggtgat gtgccggaaa gcctgcccac ccggcagagc cgctccacag
     5221 gggaaggcct cagcaactgg gtctctcgct atccgagagc gtgcctactt cgtccccgcc
     5281 tgcgcctctt caatcagctc aaacagcttc gaccggtcga tcacctcgac gaacggcgtt
     5341 ccgctcctcc ggttcgtcac cacccagatc gtcggcgtgt gctggattcc gacactttgc
     5401 cccaggtcgg tatccgcctt caccaactgg gtcagccgtc cctgcggatc caccacaaac
     5461 ggaaacttca gcttgtgatc cgcagcgaac ttctgcgcaa acgtcctcag atcgtcgagg
     5521 ctacggatcg tcggctgtgc agcaaacacc gcatctctga atgcatcgcc cagccgcctg
     5581 gacttcgtat cgaaccaccg cgcatccacc gcggcctgaa aactccacgt gtggaacgga
     5641 agcggaaaat cgtgccgcac ccagggaatg tgatacttct ccgccgcctc tttcagcacc
     5701 ggatttgccc gcgcacagtc cggacactcc agatcttcaa actcaacaat cgccacccgc
     5761 gccccagccg ggggacgcag cgcccgggcg ttatgcacca ccgtcgtctg cggctccgga
     5821 ttcgaaaact gcgccaacgc cggtaccgcc gacgctacaa acaggaaaaa gccaggaact
     5881 agaaaccgag ccagtcgaac cataagaatt ggactcattg tagcggccga acgtcctata
     5941 ctgtccgcga ttccggaaga cccacagtgg ctcgcaccgc agtcaaagcc cggcgcctca
     6001 ccattgcatc cggcgcgcgg cacgcactct gccccgatcg cccgcgttcc tggacttcga
     6061 aagacgccac ggccgccgcc caagacttct tcacccggcc gcgtcgcagc gccgcccgcg
     6121 aactgctccg tgaactctgc tctgacgatc ccttcgacca tcgctgtgcc gccgaactcg
     6181 cccgcctcgt cagccgtcgc cagcaagacg tcctcgccgc atacagcgac ctcctcgccg
     6241 aagtcgccgc cactctcccg atggaagagt ggcaggcccg cggctacgtt ctcgttgctg
     6301 ctgccatgaa tgccgtgacc cacgcccagc gcatgcgcat gctacccctc gtccgcgctc
     6361 gcctctctga agaccgtatc gccgtccgtg ccatggctct ggaagccttc gcttacctcg
     6421 ctgcccgaga gcccggcctc cgcgacgaag ccctggaaaa actcgaagcc gcccgccaca
     6481 gcagcgaacc cgccctgcgc gcccgggcaa ggctcatgct cccggtcgtg atgaagtccg
     6541 agctcacttt gccccgctaa aacacaaagg ccacccattt ccgggtggcc ttcgcttcga
     6601 accaggcaga ttcgcttctg ttttcttacc ttgccttact cttgtgcggc gcaagccgct
     6661 cccgcctcga cagctagtcg cccatgacgg gttcggcctt ctccaccggc ttggccggcg
     6721 cgatcgagtc aatcgtcacc agaccctcca ccggcttctc gcccagaatc tgctcgcgat
     6781 acagcttcgc catccgggca ttctcggcat cctgcttcag cttcgcttcg cgcgcccgca
     6841 gcttctcttc acgtgcgttc ttcgcgatgc ccagcttctc caggctgttg ttggccgccg
     6901 catccacgtg ctcctgattc agaatcagcg tgtgctgtgc ggactccatg cccaccttct
     6961 tgccgcccgc gaagatctca tgccgcccgt gctcctcgta ccacttggtg agcgtcgccg
     7021 tcatgtgcat cttctcgatg atgttccgcc agccaatgcc cgtgttgtac gcgcagaagc
     7081 tgatctcgcc ttcctgcgtc gcgtacggaa tgatgcactg ctccgtgcgc cggaagtcgt
     7141 agttgaacag gtcctggaac cacatgcccg cgatgaacag gaagttccag cggtccgcgc
     7201 gccgctgctc gatgtccgcc atggtgcggt cgcccgtcac cttgccgtag ttccggcccg
     7261 ttgctccgaa gcacttgtcg aacttctgca gcaggtcaag gatcttgaag tgcgtcggcg
     7321 acttccgcgg atcgtagttc cgcagcaacg ccagcgacac gccgaagatc gacaggaact
     7381 tcccgcgcgc cgcgtcgttc accttcgcga tgtccttcgc gaggcgatcc gcattcagga
     7441 acgccgtcac cggcaccgct tccttcgttt ccttgtcgat catcagcgcc atgccgatgc
     7501 cgcagttcgg atggcagccg cagctcagct ggccccattc gtgattcggc ccgtgcacca
     7561 gatcggccca gtcgctgaac gtgctcataa agctgatcgg gaaccagtcc cgtgtcggct
     7621 cgcccagtcc cgtctggttc ttcacgtcgt gcgccatgtg gctcagcgtg tagcgctgcg
     7681 ccaggcgccg ctcttcactc accgcctcat cgcggcctgt gaagctcacc ggctggaagc
     7741 tcaggaagtt gatcttcttc ggattgtcca gcgcgaactg gataatgtgc ccgacctgct
     7801 cgttgttaat gccgttgatg atcgtcgtca ccggcacaat atccacgccc gcttcgtgca
     7861 ggttgtggat cgcctgcagc ttcacatcga acaggttccc caccttgcgg tgggagttcg
     7921 ccgcgttgcc gatgccgtcg aactgcaggt acgcatagcg cagacccgct tccgcagccg
     7981 cccggcatag ttccttcgac ttcgcaaact caatgccgtt tgtcgccgcc tgcaccgagt
     8041 tgtacccaac cttgcgcgca tacgcgaccg cgtcgaggaa gtacggcgac agcgtcggct
     8101 cgcctcccga gaactggacg ctcatctgcc gcttcggctt aatcgtgatg gcgttgtcca
     8161 gcatcgtctt aatctcgtcc cacgtcagct catgcacaaa gccgacctgg ttggcgtcca
     8221 tgaagcatgg atcgcacatc atgttgcagc ggttcgtcag gtcaatcgtc agcaccgacc
     8281 cgcgcccgtg cgtcaccgtc gaggtgccgt ggttgtgcag cttgtcgtcg ttgtgcgcgc
     8341 gaatgtcgcg gcccggaaag acctcttcca gatgcttgaa gaacgccgtg tcgatcgaca
     8401 tcacgtcttc aaagtgcccg tgcttcgggc agtctttcac catcagaatc ttgccgtcgc
     8461 gctcaatgat ctgcgccttg atctcgccca ccttctcgtt cagaaggatc tcgtggggca
     8521 gcttgccgtc gataatctgc tggcgaatct ccggcacgca cttcggacaa agcgagtccg
     8581 tggtgcgcgg ccagccgagc ggcggcttct ccttctgcca cgacttcagc agaggcttgt
     8641 ccgaccattt cggcgtaaac gacgggttgg ggcggatgct gttcagcttc tcaaaaacgc
     8701 tccaggcccc gttggccgca atcgtaaccg ctctctcggc atatttcaca gcttttgaca
     8761 tgggtcagaa atctcctgtt gcaggatgtc gtgctctggg atgcctggac tggggctaag
     8821 tctttgcctg ccaggacttt tccagcttcc agcatagggc agggaacgca ccgcaggaac
     8881 ccggttgggt taaaagggga aaaacagcac ctggaaggtc actggggccc ctgaacggcg
     8941 caactcaatg atcgaaatgg aggttacgcc ggatcgcacc cgtcgcctcc tcgacacaac
     9001 cccgcgtcgc caaaccctcg ggtgacgatt ggaaatcatg acgggtctga ccggcaagcc
     9061 gccgtccgcc aaccgaaagg aatccgatac ctcaaatgaa gatcctcctg cgaatcgtcg
     9121 tcctgctcgc ggcgttcacg ccatccttcg gccaggggct tgaaaagggc aacctgctct
     9181 ccgtgcatgt cgtgaccgcc aaactcaatc
//
LOCUS       DASZYN010000241         4908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_391957, whole genome shotgun sequence.
ACCESSION   DASZYN010000241 DASZYN010000000
VERSION     DASZYN010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391957"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..1018
                     /locus_tag="VHE33_06140"
     CDS             26..1018
                     /locus_tag="VHE33_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVT97068.1"
                     /translation="MATTTATGQSTRFATKTLGNSDLHLTPIGYGAWAIGGGNWEFGW
                     GSQDEGEAIAAIDRALDSGLNWIDTAAVYGLGHSEEVVGKALKNRSTKPYVFTKCSMR
                     WGADRKIYRSLKKQSLQEELEASLRRLGVDAIDLYQIHWPNPEDEIEEGWEALARFRE
                     QGKVRFIGVSNFSVAQMKRVQKIAPITSLQPPYSLLRRDIEKEILPFCLENHIGVINY
                     SPMVSGLLTGKMTAERVKNLPEDDWRKRSPNFQSPKLERNLALADLLQSIGSRHGVQA
                     GVVAIAWTLRHPAITAAIVGARRPDQVDGILPAASFRLSDAEAKQIESWLTEHP"
     gene            complement(1047..2756)
                     /locus_tag="VHE33_06145"
     CDS             complement(1047..2756)
                     /locus_tag="VHE33_06145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVT97069.1"
                     /translation="MPADCYRPMTQLPTPPDFNARTSATPGAKLDFSTSESQAVFPAS
                     RGTQVSARSHDIASEAERQRAAPAQAPESATRRPEALPRGPEPELLPFLEDAVLGMHW
                     MALDGTILWANRSELELLGYDAQEFTGRNFVDFAETPETMRGVLQRLAARESIHNCEL
                     RLRVSDGSMRWVRIDASPWPPQGEVEHARCFVVDVSEKRIADEASMKLAAIVESCEDA
                     IVSKNLNGIVTSWNAAAERILGYRADEIIGKPILTLIPPELHKDEAEILRKIQAGERI
                     AHFETVRVTKSGERLDVSLTISPVRDREGNIVGAAKILRDVTMQKKMEAALRTTERLA
                     SVGRLAATVAHEINNPLEAVTNLIYLARRNPELPEGALSLLVMADEELQRVTHIARQT
                     LGFYRDTSAPIVIPVPEAIDEMLAIYHRRLTYKQVSLHKRVQPGLRVCALHGQFKQIV
                     SNLVTNAIDAVRPGGRIEVRARESRHPVTGAPGIRLVVADQGCGIPDPIRRQIFTPFF
                     TTKKDVGTGLGLWIVKGMLLKSGGTIFCRSRVARDANRDSGTVMMVFLPSDSSTQAEQ
                     AAA"
     gene            complement(3072..4169)
                     /gene="recA"
                     /locus_tag="VHE33_06150"
     CDS             complement(3072..4169)
                     /gene="recA"
                     /locus_tag="VHE33_06150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HVT97070.1"
                     /translation="MAEDRERQRAVDLALSQIEKQFGKGSIMRLGSKEAIVPISVIST
                     GSISFDAALGVGGVPRGRVVEIFGPESSGKTTITLQIIAEAQKAGGLAAFVDAEHALD
                     PAYAKKLGVDVDNLLVSQPDYGEQALEITEALVRSGAIDVLVVDSVAALVPKAELDGE
                     MGDSHMGLQARLMSQALRKLTGTVSKSRTCLIFINQIREKIGVMFGNPETTTGGRALK
                     FYSSVRIDIRRIAAVKEGDTVVGSRTKVKIVKNKVAAPFREAEFDILYGEGISREGDI
                     LDLAVANNIVEKSGAWFSYSGERIGQGRENVRQFLKDNRDTFARIDTELRKKLGISAK
                     AATAEPELPPVPPNGAAHAQEAVRGRAATRQ"
     gene            complement(4369..>4908)
                     /locus_tag="VHE33_06155"
     CDS             complement(4369..>4908)
                     /locus_tag="VHE33_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HVT97071.1"
                     /translation="IDDPVVRHLLSSLLPAMARPSDASSLFLDHVALALTAHLAHTYG
                     GVVSCGSRRGGLAPHLERRAKERMTADLREDLSLAGLAAECGLSARHFARAFRQSTGL
                     APHRWIVEHRIERARNLLASREMSLSDVALACGFADQSHFTRCFAARVGMSPGAWRRM
                     HAASGQTMADENPTTDALA"
BASE COUNT          874 a         1615 c         1455 g          964 t
ORIGIN      
        1 gccgtgaggg cgcatcgaaa acattatggc gacaactact gcaacaggac agtccacgag
       61 gtttgcgacc aaaaccctgg gaaattccga cctgcacctg accccgatcg gctatggcgc
      121 atgggcgatt ggcggcggca actgggaatt cggctggggc tcccaggatg agggcgaagc
      181 gattgccgcg atcgaccggg cgctcgacag cggcctgaac tggatcgaca ctgccgccgt
      241 ctatgggctc ggacactcgg aagaggtcgt cggcaaggcc ctgaaaaatc gctcgacgaa
      301 gccgtatgtc tttacgaagt gctcgatgcg gtggggtgcg gatcgaaaga tctatcgcag
      361 cctgaagaag cagtctctcc aggaagagct tgaggcatcg ctgcgccggc tgggcgtgga
      421 tgcgatcgac ctctatcaga tccactggcc gaatcccgag gacgaaatcg aagaaggctg
      481 ggaagcgctg gcgcgcttca gggagcaggg caaggtgcga ttcatcggcg tatcgaattt
      541 cagcgtggcg cagatgaagc gcgtacagaa gattgctccc atcacgtcgc tgcagccgcc
      601 gtattcgttg ctgcgccgcg atatcgaaaa ggaaatcctg cccttctgcc tggagaatca
      661 catcggcgtg atcaactact caccgatggt ctccggactg ctgaccggga agatgacggc
      721 cgagcgggtg aagaatctgc cggaggatga ctggcggaag cgcagcccga atttccagtc
      781 gccgaagctg gagcgcaacc tcgccctcgc cgatctgctg cagtcaatcg gcagccggca
      841 tggggtccag gccggtgtgg tggccattgc ttggacgtta aggcatccgg caatcaccgc
      901 cgccatcgtt ggcgcgcgcc ggcccgatca ggtggatggc atcctgcccg cggcaagttt
      961 ccggttgagc gatgcggagg ctaagcagat cgaaagttgg ctgaccgaac atccgtagtc
     1021 gagggcggga agggaagcag cgagcctcag gcggcagcct gctccgcctg ggtgctcgaa
     1081 tcgctcggca ggaacaccat catcacggtt ccggaatctc tgttggcgtc ccgcgctaca
     1141 cggctgcggc aaaagatggt gccgcctgac ttcagcagca ttcctttcac aatccacagc
     1201 ccaaggccgg tccccacgtc cttcttcgtg gtgaagaacg gagtgaagat ctgccgccgg
     1261 atggggtcgg gaattccgca tccctgatcc gcaaccacca gccggatccc cggcgcgccc
     1321 gtgaccgggt gcctcgattc ccgcgcccgc acttcaatcc gtcctcccgg ccgcaccgcg
     1381 tcgatcgcgt tggtcacgag gttggagacg atctgcttga actggccgtg caaagcgcaa
     1441 acgcgcagac ccggctgcac ccgtttatgc agcgagacct gcttataggt gagccgccgg
     1501 tggtagatcg ccagcatctc gtcgatggct tccggcaccg gaatcacgat cggtgcggat
     1561 gtatcgcggt agaaacccag ggtctggcgc gcgatgtgtg ttacgcgctg cagttcttca
     1621 tcggccatca ccaggagtga cagcgctccc tccggcagct ccggattccg gcgtgccagg
     1681 taaatcaggt tcgttaccgc ctcgagcgga ttgttgatct catgtgccac cgtcgctgcc
     1741 agtcgaccca ccgacgccag gcgctcggtg gtgcgcagcg ccgcttccat cttcttctgc
     1801 atcgtcacat cgcgaagaat cttcgccgcg ccgacgatgt tgccctcgcg atcgcggacc
     1861 ggcgagatgg tcagtgacac atccagccgc tctccgctct tcgtcacgcg aaccgtttca
     1921 aaatgcgcga tgcgctctcc cgcctgaatc ttgcgcagga tttcagcttc atccttgtgc
     1981 aattcgggag gaatgagagt caggatcggt ttgccgatga tctcgtccgc tcgatatccc
     2041 aggattcgct cggcggctgc attccagctg gtgacgattc cgttcagatt cttgctgacg
     2101 atggcgtcct cgcaggactc gacgatcgct gccagcttca tcgatgcctc gtccgcgatc
     2161 ctcttttcgc tcacgtcgac tacgaagcag cgcgcgtgct ccacttctcc ctgcggtggc
     2221 cagggactgg cgtcgatgcg tacccatcgc atcgaaccgt cgctgacccg cagccgcagc
     2281 tcgcagttgt gaatgctttc ccgcgccgcc agccgttgca gaaccccgcg cattgtctcc
     2341 ggcgtttcgg caaaatccac gaaattacgg cccgtgaatt cctgcgcgtc atagccaaga
     2401 agctccagtt cgctgcgatt tgcccagaga atcgtcccat ccagagccat ccagtgcatg
     2461 cccagcacgg catcttcgag aaaggggagt agctcaggct ccgggccccg gggcagcgcc
     2521 tccggtcggc gagtcgcgct ctccggcgct tgggccgggg cggcgcgctg ccgctctgcc
     2581 tcgctcgcga tgtcatggct gcgggctgag acctgggtgc ctcgactggc ggggaaaaca
     2641 gcctgactct cagaggtgga gaaatccagc ttcgcgcccg gcgtcgccga ggttcgtgcg
     2701 ttgaagtccg gcggagtcgg gagttgtgtc atcggcctgt agcagtcggc aggcacacga
     2761 acttctgctc tgtctgcaat gttcttgaga tggccccttc cggatccggg atgcccacca
     2821 ccccgcggag cgccgccgag ggtcgagaat cccgcccgat cacgcctcct gtgcagcgtt
     2881 tcccggtgga tcgcagccgc tcggcgataa acctgagttt gatcgaatgc gcggagcgga
     2941 agaccgcatt cagcgtctcc gtctcgaatc gctttgccga tcccggaaaa gcaaatggcc
     3001 ccgccggggc catttgcttt tcactctcgc tctgctctgc ggtgttcccg gtttcggctc
     3061 cgggcccgcg tttattgccg cgtcgctgcc cgcccgcgca ccgcttcctg cgcgtgtgcc
     3121 gctccgttcg ggggaaccgg cggaagctcc ggttcggccg ttgccgcctt tgcgctaatc
     3181 cccagcttct ttcgcagctc cgtgtcgatc cgcgcaaacg tgtcgcggtt gtccttcagg
     3241 aactgccgta cgttctcccg tccctgtccg atccgctcgc cggagtagct gaaccacgct
     3301 ccgctcttct ccacaatgtt gttggcgacc gccagatcca ggatatcgcc ctcgcgcgaa
     3361 atcccttcgc cgtaaagaat gtcgaactcc gcctcgcgga acggcgcggc aaccttgttc
     3421 ttcacgatct tcaccttggt ccgtgatccg acgactgtgt cgccttcctt cacggccgcg
     3481 atgcggcgga tatcgatgcg caccgacgag tagaacttca gcgcgcggcc gcccgtcgtt
     3541 gtctccgggt ttccgaacat cacgccaatc ttctcgcgga tctggttaat gaaaatgagg
     3601 cacgtccgcg acttggacac tgtcccggtc agcttccgga gcgcttgcga catcagccgt
     3661 gcctgcagcc ccatgtgcga gtcgcccatc tccccgtcca gttccgcttt gggaaccagc
     3721 gccgccaccg agtccaccac cagcacgtcg atcgcccccg accgtaccag cgcctccgtg
     3781 atctccagcg cctgctcgcc gtaatccggc tgcgacacca gcagattgtc gacatccacg
     3841 cccagcttct tcgcgtatgc gggatcgagc gcatgctcgg catcgacaaa cgcagccagg
     3901 ccgccggcct tctgcgcctc ggcgatgatc tgcagagtaa tcgtggtctt gcccgacgac
     3961 tcaggaccaa agatttcgac gacgcgaccc cgcggcacac cacccacacc cagagcggcg
     4021 tcgaaggaga tcgagccggt cgaaataacg gagatgggga cgatggcttc cttgctgccc
     4081 agccgcataa tcgacccttt gccgaactgc ttctcaatct gcgatagcgc caggtctacc
     4141 gcccgctgcc gttcacgatc ctctgccacg ttgctgctcc attccgggct tttcgcatgc
     4201 gtgatagccc tggttcgtcg tctggacttt cgcctaatct ttgccttgct ctttcgagtc
     4261 taccatcgga caacaaccgc tccacccctc aacgcaacac aattttcacg gaggtaatgg
     4321 cgcagttatt tttccgagtc taccaggttt ctttggtacc gtttttcctc aggccagggc
     4381 gtccgtcgtc ggattttcat cagccatcgt ctgccccgac gcggcgtgca tccgccgcca
     4441 ggcgccgggg ctcatcccca cccgggctgc aaaacatcgc gtgaagtggc tctgatctgc
     4501 gaacccgcac gcgagcgcca catcgctgag tgacatctcc cggcttgcca gcagattccg
     4561 cgcccgttcg atgcggtgct cgacgatcca gcgatgcgga gcaaggcccg tcgactgccg
     4621 gaatgcgcgc gcgaagtgcc gggctgaaag tccgcattct gcggcgaggc cggccagcga
     4681 caagtcttct ctcaggtcgg ccgtcatgcg ttccttagcg cgccgttcga ggtgcggcgc
     4741 aagtcctccg cgccttgaac cgcacgagac cacgccgcca taggtgtgcg ccaaatgagc
     4801 cgtcagcgcc agggccacgt gatcgaggaa caaagaggat gcgtccgaag gtcgcgccat
     4861 ggcgggaagc agcgacgaga gcaaatgacg caccaccggg tcatcgat
//
LOCUS       DASZYN010000242         2602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_586550, whole genome shotgun sequence.
ACCESSION   DASZYN010000242 DASZYN010000000
VERSION     DASZYN010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586550"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1114)
                     /locus_tag="VHE33_06160"
     CDS             complement(158..1114)
                     /locus_tag="VHE33_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT97072.1"
                     /translation="MSATAVLTPTATAWPAPLSQTRTIAKLHSVTKRYGQTIALENFS
                     MELRAGEVVALLGPNGAGKTTAVRLLLGLIAANAGTVRVFGRDPRDADARTRIGAMLQ
                     VTRVPEMLRVREHIDLFRTYYPNPLPADEVIRIAKIESIADQMFGKLSGGQKQRTLFA
                     LALCGNPDLIFLDEPTVGMDIESRRALWDEVRALSAAGKTVLLTTHYLEEADMLAHRI
                     VVINKGHVVCEGTPAEIKHRVSGRRIRCITQLDPEFLATLPSVADVQQDREAVVILAE
                     HPEQVVREILLRDETLHGLEVSAAALEDAFLALTSNHEPAQA"
     gene            complement(1378..1812)
                     /locus_tag="VHE33_06165"
     CDS             complement(1378..1812)
                     /locus_tag="VHE33_06165"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HVT97073.1"
                     /translation="MKTFTLDEAQSLLPVVESLLNRALEAKQAASDLQETISNQARRI
                     FVSGGMFVDIAAAQKRRIALDALVQRAKDAIEEIDAAGVQIKDLDIGLLDFPFQLEGE
                     TVLLCWKRGEPRIEFWHRMEDGFAGRQPIDARFGGLPSDKLN"
     gene            complement(1844..>2602)
                     /gene="nadA"
                     /locus_tag="VHE33_06170"
     CDS             complement(1844..>2602)
                     /gene="nadA"
                     /locus_tag="VHE33_06170"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HVT97074.1"
                     /translation="ECWEALHKAGAREGEIIPLTYMNSSAAIKAFCGERGGLVCTSSN
                     ARKAFEWAFARGEKILFLPDQHLGRNTGHAMGIPLNEMPVYDPWQIGGGVTADKLHAA
                     SVILWKGHCSVHQRFLPEHVDKVRAKYPGIQVIVHPECRLEVCQKADALGSTERLIKI
                     VQEAPEGTTFAIGTEIHLVNRLAKQFAPLGKKIVTLDDTGCLCTTMYRISPGHLAWAL
                     ENLVEGRVVNRIRVRDDVKYWARVALDRMLEVRA"
BASE COUNT          464 a          677 c          964 g          497 t
ORIGIN      
        1 cgatgacgga gagcgagaag gtgcgcgtgc gcagcatgcg gacaaattcg tactgggctt
       61 ccttgcggaa gatgcgcgcg taatagccga aggtgagggg gggcgcggcg atgattgtgt
      121 tggtggccat gattcttctc ctgtcggtgt tcttcgctta ggcctgggcc ggttcgtgat
      181 tgctggtgag ggcgaggaag gcgtcttcga gagcggcagc ggagacttcg aggccgtgga
      241 gggtctcatc gcgcaggaga atttcgcgga cgacctgctc ggggtgctcg gcgaggatga
      301 cgacggcttc gcgatcctgc tgaacgtctg cgacggaggg cagggttgcg aggaattcgg
      361 ggtccagctg cgtgatgcag cgaatgcgac ggccggagac gcggtgcttg atttcggcag
      421 gtgtgccttc gcacacaaca tgccctttgt tgatgaccac gatgcggtgg gccagcatgt
      481 cggcttcttc gaggtaatgc gtggtgagca ggacggtttt gccggcggcg gaaagagcgc
      541 gaacttcgtc ccagagggcg cggcgggact cgatgtccat gccgacggtg ggctcgtcga
      601 ggaagatgag gtcgggattg ccgcaaagag cgagagcgaa gagagtgcgc tgcttttgtc
      661 cgccggagag cttgccgaac atctggtcag cgatggactc gatcttcgcg atgcgtatga
      721 cttcgtcggc gggcagcgga ttgggatagt aggtacggaa gaggtcgatg tgctcgcgga
      781 cgcgcagcat ttccggaacg cgggtgacct ggagcatggc gccaatgcgg gtgcgagcgt
      841 cggcgtcgcg gggatcgcga ccgaagacgc ggacggtgcc ggcattggcg gcgatgaggc
      901 cgagcagcag acgcacggcg gtggtcttcc ctgctccgtt ggggccgagc agggcgacga
      961 cttcgccggc gcggagttcc atagagaagt tctcgagcgc gatcgtctgg ccatagcgtt
     1021 tggtgacgga gtggagcttt gcgatagtgc gggtctgcga tagcggagcg ggccaggcgg
     1081 ttgcggttgg cgtcaggacg gcggttgctg acattgcgtc tctcctgcca ccgggactcc
     1141 agcggcaaag acaggatcgc gcggaagggt gtttgcgggc agtggggaat gtcaggaaag
     1201 gagggtgaca aatgtcaggg cgcagatcgc aggggtcagg tcgcagggca cagggctcag
     1261 ggctcagggc agaagcaggt tcctcggctc accgcccccc aaactgaaaa acgtttaggg
     1321 cccctttcgc tcggaatgac aattccgatt catgtcagga aattcaggga caacgcgcta
     1381 gttgagtttg tcggagggga ggccgccgaa gcgggcgtcg atgggctggc ggcccgcgaa
     1441 gccgtcttcc atgcggtgcc agaactcgat gcgcggctcg cctcgcttcc agcagaggag
     1501 cacggtttcg ccttcgagct ggaaggggaa atcgaggagt ccgatgtcga ggtccttgat
     1561 ctgaacgccg gcggcgtcga tctcctcaat cgcgtccttt gcgcgctgca cgagggcgtc
     1621 gagggcgatc ctgcgcttct gggcggcggc gatgtcgacg aacatgccgc cgctgacgaa
     1681 gatacgccgc gcctgattgc tgatggtttc ctgcagatcg gaggcggcct gtttggcttc
     1741 gagggcgcgg ttcagcagcg actccaccac cgggagcagg gactgcgctt cgtccagggt
     1801 gaaagtcttc ataggggcaa ctgcaggcgc ggaggcgtcg cgatcaggcg cgaacctcga
     1861 gcatacgatc gagcgccaca cgagcccagt atttcacatc gtcccggacc cgaatccggt
     1921 tgacgacgcg gccttcgacg agattttcaa gcgcccaggc aagatgtccg gggctgatgc
     1981 gatacatcgt cgtgcacagg cagccggtgt cgtcgagggt gacgatcttc ttgccgagcg
     2041 gcgcgaactg ttttgcgagg cgattgacga ggtggatttc agtgccgatg gcgaaggtcg
     2101 tgccttcggg cgcttcctga acgattttga tgaggcgctc ggtggaaccg agagcgtcgg
     2161 ccttttgaca gacttcaagg cggcactccg ggtgcacgat gacctgaatg ccgggatatt
     2221 ttgcgcggac cttgtcgacg tgttcaggaa ggaagcgctg atggacggag cagtggccct
     2281 tccagaggat gacgctggcg gcatggagct tgtccgcggt gacaccgccg ccaatttgcc
     2341 atggatcgta gacgggcatt tcgttgaggg gaatgcccat ggcgtggccg gtgttgcggc
     2401 cgaggtgctg gtcggggagg aagaggattt tctcaccacg ggcgaaggcc cactcgaagg
     2461 ccttgcgcgc gttggaggaa gtgcagacca ggccgccgcg ctcgccgcag aaagctttga
     2521 tggccgccga ggaattcatg taggtgagcg ggatgatttc gccttcgcgc gcgccggctt
     2581 tgtgcagagc ctcccagcat tc
//
LOCUS       DASZYN010000243         2737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1163935, whole genome shotgun sequence.
ACCESSION   DASZYN010000243 DASZYN010000000
VERSION     DASZYN010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163935"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VHE33_06175"
     CDS             <1..523
                     /locus_tag="VHE33_06175"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HVT97075.1"
                     /translation="EPWLRGTHTEMPAALRAVIHALELAREDIDQWCLPLSDEAINAR
                     PGPDSDPIAPVAFHLRHIVRSLDRLLTYAEGEQLSPAQLAALKTELDPGAAHAELFSE
                     FRKGIDDAIRRVEAFAGADLDQPRAVGRKALPTTVGGLLVHCADHTQRHTGQAITTAK
                     LAQNLQSSRGAES"
     gene            complement(591..1706)
                     /locus_tag="VHE33_06180"
     CDS             complement(591..1706)
                     /locus_tag="VHE33_06180"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HVT97076.1"
                     /translation="MSLSRRSFVQLAGSGLLLQSQLLQAERSLRSGIQGVPVQAPGTT
                     EPAKVALVHGEDRRRNVRAALETIDGEIRAGLRGKKSVLIKPNNVSTTNQLAATHLDA
                     LEGILDYLESRFHGPVVIAESSAGDTLEGFEHFGYNRLAGERRAQKVSLVDLNREAKY
                     GTVEVLDADLHITPVRLAARVLDPETFVISSAMLKTHNTVVATMSVKNMVLGSPLHSA
                     PGELFWSDKRKYHVGIRQTHYNMLVTAQKLQPHWGLAVIDGYEGMEGDGPGNGTPVAS
                     NVAIASTDLIAADRVGLEVMGINPDWVGYLAYCGQAGLGQYDLAKIRVEGASIASVTQ
                     KYKLHPQIQKELEWMGPLQKGSPTMGSLLMGDETYPA"
     gene            1891..2385
                     /locus_tag="VHE33_06185"
     CDS             1891..2385
                     /locus_tag="VHE33_06185"
                     /inference="COORDINATES: protein motif:HMM:NF018951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdC"
                     /protein_id="HVT97077.1"
                     /translation="MIHISPLTYAVTSVFGAVAVIVWRIREGQTPVTLKKILIPPAGM
                     ATGFSMFVVPLFRIPWLWALVAFLIGAIFLAWPLLRTSRLHREGDVIMMKRSSMFLVV
                     IILLAAVRFAARSYFDRIMTVEQTGGLFFILAFGMILRWRMNMFFEYRTLTNLPGNFD
                     HSAA"
     gene            complement(2436..>2737)
                     /locus_tag="VHE33_06190"
     CDS             complement(2436..>2737)
                     /locus_tag="VHE33_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HVT97078.1"
                     /translation="PSEHTMNVWAFAHVVAGQYNGIATQALVYGAATTVSVSRVLSRE
                     HFPSDVVVGSTLGWLIGGYVLHHRSLEHGTLLDVSTVETPLGSGVQVRLNLARGK"
BASE COUNT          476 a         1016 c          713 g          532 t
ORIGIN      
        1 ggaaccctgg cttcgcggca cgcacaccga aatgcccgcc gccctgcgcg ctgtcatcca
       61 cgccctcgaa ctcgcccgtg aggacatcga ccaatggtgc ttaccgttga gcgacgaagc
      121 aatcaacgcc cggccgggcc ccgattccga tccgatcgct cccgtcgcct ttcatcttcg
      181 ccacatcgtg cgcagtctcg accgcctgct cacctatgct gaaggcgagc agctcagtcc
      241 tgctcaactc gccgcgctta agacagagct cgaccctggc gctgcgcacg ccgagttgtt
      301 ttcagaattt cgaaagggaa ttgacgacgc cattcgccgc gtcgaagcct ttgccggcgc
      361 cgatctcgat cagccccgcg ctgtcggccg caaagctctg cccaccacgg tcggcggcct
      421 cctcgttcac tgcgccgacc acacccagcg ccacaccggc caggcgatta cgacggcaaa
      481 gctcgcccag aatctccagt cgagtcgagg agctgagagt tgatagttga gagttgtgag
      541 tgcgcccaaa ccattcagcc ctcgcaactc acaactccca actgtgtcct ttacgccgga
      601 tacgtctcat cccccatcaa caagctgccc atcgtcggcg accccttctg cagcggcccc
      661 atccactcga gttccttctg gatctgcgga tgcagcttgt acttctgcgt caccgacgca
      721 atcgacgcgc cctccacccg gatcttcgcc aaatcatatt gccccagccc cgcctgcccg
      781 caataggcca ggtacccaac ccagtcggga ttgatcccca tcacttccag cccaacgcga
      841 tccgccgcga tcaaatccgt cgacgcgatc gccacattcg atgccaccgg cgtgccgttg
      901 ccagggccgt cgccctccat cccctcgtac ccgtcgatga ccgccagccc ccagtgcggc
      961 tgcagctttt gcgcggtcac cagcatgttg tagtgcgtct gccggatacc gacatggtat
     1021 ttgcgcttgt cgctccagaa caactcgccc ggcgcgctgt gcagaggcga tcccagcacc
     1081 atgttcttca cgctcatcgt tgccaccacg gtgttgtgcg tcttcagcat cgcgctcgag
     1141 atcacaaacg tctccggatc gagcacccgc gccgccagcc gcacgggcgt gatgtgcaga
     1201 tcggcatcca gaacctcgac ggtcccatac ttcgcctcgc ggttcagatc caccaggctc
     1261 accttctgcg cgcgccgctc gccggccagc cggttgtagc caaaatgctc gaacccctcc
     1321 agcgtgtcgc ccgccgacga ctcggcaatc accaccggcc cgtgaaagcg agactccaga
     1381 taatcgagga ttccttccag cgcatcgagg tgggtcgccg ccagctgatt tgtggtcgac
     1441 acgttattcg gcttgatcag caccgacttc ttcccgcgca gacccgcgcg aatctccccg
     1501 tcaatggttt ccagcgcggc acgcacgttc ctgcggcggt cctctccatg cacgagcgcg
     1561 accttcgccg gttccgtcgt ccccggcgcc tgcaccggaa ccccctggat ccccgaccgc
     1621 agcgaacgct ccgcctgcag cagctgcgac tgcagcagca aaccggaccc cgccagctgc
     1681 acaaaactcc gccgtgacag actcatagcg cgcctcctga aggcacttct ggttatccgc
     1741 tatgttctgc gctggatgct gcccagttcc gtgaccgcca tcacggcaat cagcctgcaa
     1801 gctgagacct cagacctgag acctgcgatc ggtccctgtt ccctgtatcc tgattcgtag
     1861 gccgccccag attccaggcc tgcgcagtcc atgatccaca tctccccact gacttacgcg
     1921 gtcacttcgg tctttggcgc cgtcgcggtc atcgtctggc gcatccgcga gggccagact
     1981 ccggtcacgc tcaaaaagat cctcattcca ccggctggca tggcgaccgg attctctatg
     2041 tttgtcgtgc ccctgttccg catcccgtgg ctctgggcgc tggtggcctt cctgatcggg
     2101 gccattttcc tggcgtggcc gctgctgcgc acctcgcgcc ttcatcgcga gggcgacgtc
     2161 atcatgatga agcggtccag catgttcctg gtggtgatta tcctgctggc tgccgttcgc
     2221 ttcgcggcgc gcagctattt cgaccgcatc atgaccgtcg agcagactgg cggactcttc
     2281 ttcatcctcg ccttcggcat gatcctgcgc tggcgcatga acatgttctt cgaataccgg
     2341 accctgacca accttcccgg caatttcgac cattccgccg cctgatcggc gagtacggct
     2401 gggaaggggg gctgactcgc ggactggctc cgggctcact ttccgcgagc aagattcaat
     2461 cgcacctgca ctccgctccc cagcggcgtc tccaccgtcg acacatccag cagcgttccg
     2521 tgctccagcg aacggtgatg cagcacatag cctccgatca gccagcccag tgtgctcccc
     2581 accacgacat cggacgggaa atgctcccgc gacagcacgc gcgacaccga caccgtcgtc
     2641 gccgcgccat agaccagcgc ctgcgtcgcg attccgttgt attgccccgc caccacatgc
     2701 gcgaacgccc aaacgttcat cgtgtgttcg gagggca
//
LOCUS       DASZYN010000244         8924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_521727, whole genome shotgun sequence.
ACCESSION   DASZYN010000244 DASZYN010000000
VERSION     DASZYN010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8924
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521727"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VHE33_06195"
     CDS             <1..811
                     /locus_tag="VHE33_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97079.1"
                     /translation="TARRLHSMMGNTRPSGGELSGPAYTLNVAGTELLLDLGAPLYDK
                     NGARLAPVDVVLETDSRNPPAKHRILELYAEHGFQFPDTAPMALHRGRIDLLEQHLRR
                     DPALRTSTFTFAEIYPPELGCHDEILATHGTPLAGATLLHMCADYNEIEIARWLLDRG
                     MNPDQPARTDSDGFGGHTALFSTVVSQANFWTNQRGQSSDAAITRMLLARGANPNARA
                     SLRKQLHPGYGIDGVHEYRHVTPLGWGEEFIFKALVDQTALRLIADYGGGR"
     gene            complement(838..1128)
                     /locus_tag="VHE33_06200"
     CDS             complement(838..1128)
                     /locus_tag="VHE33_06200"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HVT97080.1"
                     /translation="MAEDCSHLGTIQVKTTTTHVCPECVKMGDRWVHLRLCLECGHVG
                     CCDSSKNKHATKHFHATRHPLIRSIEPRESWVWCYVDEISPGEIRNGEFLAA"
     gene            complement(1179..2096)
                     /locus_tag="VHE33_06205"
     CDS             complement(1179..2096)
                     /locus_tag="VHE33_06205"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HVT97081.1"
                     /translation="MTKIPLPDDPNCWPPLPLDEWNDTRATLHMWTQIVGKIRLKLTP
                     LVNHWWNVPLYVTARGLTTSRIPYASRAFELRFDFLAHQLVLETSEGTRRTIALQPQT
                     VADFYQSCMDMLRREGIEAAIWPMPVEVPDPIPFDQDSVHTAYDPVMVEKFWRILLSV
                     DAVFQDFRSEFIGKVSPVHFFWGSFDLAVTRFSGRRAPERPGADPITREAYSHEVSSV
                     GFWPGGGNVPGAAFYSYAAPVPPGFAEARVRPDVAKWDKGLGEFLLMYDDLRKSPSPS
                     RALLEFCRSTYQAAARFGQWDRGALERTA"
     gene            complement(2189..3712)
                     /locus_tag="VHE33_06210"
     CDS             complement(2189..3712)
                     /locus_tag="VHE33_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HVT97082.1"
                     /translation="MAAIRQEDFIQSVADALQYISYYHPVDYITSLARAYEREQSLAA
                     KDAMAQILVNSRMSAEGHRPICQDTGIVTVFLKIGMQVRWDATMTVQEMADEGVRRAY
                     RNPDNTLRASILADPAGARKNTTDNTPAVVSIEMVKGGEVDVTVAAKGGGSEAKSKFA
                     MLNPSDSIVEWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDMRELL
                     DRGPKTRLEEMRIEIYEKVNQLGIGAQGLGGLTTVLDVKILDYPTHAANLPVAMIPNC
                     AATRHAHFVLDGSGPVALDPPSLQDWPQLTYDVSSARRVNLDTVTREQAATWKPGEVL
                     LLRGKLLTGRDAAHKRMTDMLNRGEKLPVDFTNRFIYYVGPVDPVREEVMGPAGPTTA
                     TRMDKFTRQMLEQTGLLGMVGKAERGPAAVEAIRDNRAVYLMAVGGAAYLVSKAIRAA
                     RVVAFDDLGMEAIYEFQVEDMPVTVAVDAQGTSVHETGPREWKAKIQTEFAGVPILA"
     gene            3959..6421
                     /locus_tag="VHE33_06215"
     CDS             3959..6421
                     /locus_tag="VHE33_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT97083.1"
                     /translation="MNSLTQDLRYALRQLRKSPVFALTAVITLGLGIGVNAAMFSVIE
                     QVLLRPLPYHSASQLVMIAPHPVTGGGFDPASLPDVRDWKTRAHVFSDLAWWTIQVPT
                     LGGRDNPELVAQISSSANLLEVLGVQPAIGRGFVPEDAKQNRAQVVILGDSTWHKVFH
                     ADRSVIGRVVPINGDPYTVIGVMPPNFEFPQTTGDEIYSPLPNDSSFEDRGNSSISAV
                     ARLRPGISVQQAQQEINGIHAQLLHEYANESTEPVRVESLRDVATDNVRSGLYALDAA
                     VAVVWLIACANLAGLMLARANARRRELAIRGALGAAKSRLVRQFLTESLLVSLVGGAA
                     GLVAAWVSLRILSHYLSNAVPFGEHIHIDAGVCAILILLSCLSAVFFGVGPGLYGANL
                     PVQEGLRDGAAASGTSKKHARWRDALVVGEIGLTLTLLIAAGLLIQTLWQLRHTNLGF
                     APEQVDATSIFMPTHGAWWTGGDKTAPSIVTGFYDPLQEKLRHTPGIQSVGMATIRPF
                     TSNHFTLDIWPADRPQPSKADAMGAAVRASNADFFRTMGIAITSGRGFEETDHAGAPL
                     AVVVNQEFVRRILKGRDPLGVRLKWDDEDKALTATIVGTIADLHQESVRAGSAPEIYF
                     NLEQLVPGQDIYSILAGFHMDIVVRTNLASEVAFRAVRDAVHELNPNLAFAEQTTMQQ
                     VIDNSLGAQTLAARLLAIFGFVALLIAIAGIYGLLAYSVSQRTRELGLRIALGAQREN
                     ILWLVLRHAILLLATGMAIGLGVAWAASSVLRSFLYGAAAYDAATVVLVVLILGLCSM
                     AASYLPARRAAAVDPIEALRTE"
     gene            6466..>8924
                     /locus_tag="VHE33_06220"
     CDS             6466..>8924
                     /locus_tag="VHE33_06220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HVT97084.1"
                     /translation="MFTATQDFHFALRQLRKSPGYAAVAIVTLALGIGANTAIFLLTY
                     SILLRSLPVPHPSELIRYTFRKGESDIGLSYPQYQALQKRQGIATGLFAWQSGEATLT
                     RNGHSQKIPVGLTTGSAFPILQLHPALGRGFAPNVGEKGNGWQPEALLSYDYWRSAFH
                     ADSAIVGQSIRLENTSITIIGVLPRGFDGIFPERPIDVLLPLDFEGILHPKNSMLYQP
                     GAFWLTVMGRLRSGETMQAAQANLAAIQAPVTEAADPSHNFLTTGFFADFRLGVESGR
                     GGRSFLRWRYAKPLLALEALCGLMMLLCGVNVALVVVSRVSSRLHEFAMRSALGASRR
                     RLLLQVLTETLLLGAGGLAGGALLGWEMARGLVSMISGPAGPDAVQLQAGAVVFLFAA
                     VVSLGAALIAGLWPAWRASHTAPALDLKQTASARTAHRLGRWIIPSQVALGVVLLNAA
                     LLLAGTLSSYLRENSGFNADQTSLTEIDVANLGVPIPNSAARAMDLLREVEGAPGIRS
                     AALMSLAPINGGFSVGGYYTLDPKGNRISNQQVWPETASTSYFDVMGTRILEGRGFQA
                     SDASGESICVISAAAATFFFPGRSALGGLLNSGDGSEKPVDRATCRVVGIAEDARMRS
                     LLEPPPLAVYLPLRDAKDFSLTYATLGVRAANPALATAAIRQAFTRVFPGAPAPRIWR
                     FQDAERYDLSRQRLLSSVSGGFALLALALVATGLYGILSRAVTERRREIGIRMALGAR
                     RQQIVSTLARGTALRILVGVIAGIGLAAAAGRLLQSLLYGVTPGSPLVALATFAVLMA
                     VLVLAFVFPAGRAASVDPMEA"
BASE COUNT         1540 a         2925 c         2618 g         1841 t
ORIGIN      
        1 aaccgcgcgc cgattgcact cgatgatggg gaatacccgg ccttcgggcg gcgaactaag
       61 cggccccgcc tatacgctca atgtcgcagg cacggagttg ctgctggatc ttggcgcccc
      121 tctctatgac aagaacggag cgcgactggc gcctgttgac gtcgtacttg aaaccgacag
      181 ccgcaatcct ccggcgaaac atcgcattct cgaactgtat gcagagcacg gattccagtt
      241 tccggacacg gcgccgatgg cgctgcaccg aggccgcatc gatttactcg aacagcatct
      301 gaggcgtgat cccgcgctcc gcaccagcac attcacgttc gctgagatct atcctccgga
      361 gcttggatgt cacgatgaga tcctggccac acatggcacg ccgctcgccg gcgcgacgct
      421 gctgcatatg tgcgccgatt acaacgaaat cgagatcgcc cgctggctgc tggatcgcgg
      481 catgaacccg gaccaacccg ctcgcaccga ttccgacggt tttggcggac atacggcgct
      541 cttttcgacc gtggtctcgc aggcaaattt ttggacgaac cagcgtggac aatcttcaga
      601 cgccgccatc acgcgtatgt tgcttgcccg cggagcgaat ccaaatgccc gagcctcgct
      661 gcgcaagcag cttcatcccg gatatggaat cgacggagtc catgaatacc ggcacgtgac
      721 tccgctgggc tggggtgaag agttcatctt caaagcgctc gtcgatcaaa ccgcccttcg
      781 cctcatcgca gattatggcg gcggacggta gatcgggcgg ctgagccact gccaggctca
      841 cgccgccaga aattctccgt ttctgatctc gcccggcgag atttcatcga cgtagcacca
      901 gacccagctt tcccttggct caatcgaacg aatcagcgga tgccgggtcg catgaaagtg
      961 cttcgtcgca tgcttattct tcgaggagtc gcagcagccc acatggccgc actcgaggca
     1021 caggcgcaga tgcacccagc gatcgcccat cttcacgcac tccggacaca catgcgttgt
     1081 ggtcgtcttc acctgaatcg ttcccagatg cgaacaatct tctgccattc ctgatcctcc
     1141 ttcggttttt aacctgggcc cgactgcatg gttgccacct acgcggttcg ctccagcgcg
     1201 ccgcgatccc attggccgaa tcgcgccgct gcctgatagg tgctccggca gaattcgagg
     1261 agcgcgcggc tgggcgaggg gctttttcgc agatcgtcat acatcagcag aaactcgccg
     1321 aggcccttgt cccacttcgc cacatcggga cgaacccgtg cctcggcaaa tccgggcggg
     1381 accggggccg cataggaata gaatgccgcg ccgggaacat tgccgccgcc cggccagaag
     1441 ccgacgctgc tcacttcatg cgaatacgcc tcgcgcgtga tcggatccgc gccgggacgt
     1501 tcgggcgccc tgcggccgga gaagcgcgtg acggccaggt cgaagctacc ccagaaaaag
     1561 tgcaccgggc tcaccttgcc gatgaactcc gaacgaaagt cctggaagac cgcgtcgacg
     1621 ctcaacagga tgcgccagaa cttctccacc atgaccggat cgtaagcagt gtgcacggag
     1681 tcctggtcga aggggatcgg atcgggcacc tcgaccggca tgggccagat cgcggcctcg
     1741 atgccttcgc ggcgcagcat gtccatgcac gattggtaga agtccgcgac cgtctggggc
     1801 tgcagtgcga tcgttcgtct ggttccttcg ctggtttcaa ggaccagttg atgtgccaga
     1861 aaatcgaagc gcagctcgaa cgcacgcgac gcatagggaa tgcgcgaagt cgtcagcccg
     1921 cgcgccgtca catacagcgg cacgttccac cagtggttca ccagcggcgt gagcttgagg
     1981 cggatcttgc cgacaatctg cgtccacata tgcagggtcg cgcgggtatc gttccactcg
     2041 tcaagcggca gcggcggcca gcaattcgga tcatccggca gaggaatctt cgtcatgagg
     2101 accttatttt ctccctatgg acacccgagg ttccaaaatc aatgctggcc gaggacgtga
     2161 agcaggcgtc cgcccgggca gcgagccctc aggccaggat cggcacgccc gcaaactctg
     2221 tctggatttt tgccttccac tcgcgtgggc ccgtctcgtg cactgatgtt ccctgcgcgt
     2281 cgacggcgac ggtcaccggc atgtcctcga cctgaaattc atagatcgcc tccatgccca
     2341 ggtcatcgaa tgcaacgacg cgcgctgcgc ggatcgcttt cgagacgaga taggccgcgc
     2401 cgcccaccgc catcaaatag acggcgcgat tgtcgcgaat cgcctccacc gccgcaggcc
     2461 cgcgctctgc cttaccgacc atccccagaa gtccggtctg ttccagcatt tggcgggtga
     2521 atttgtccat gcgagtggcg gtcgtcggcc cggccggtcc catgacttcc tcgcgtaccg
     2581 gatcgaccgg cccaacgtaa tagatgaagc gattggtgaa gtccaccggt aacttctcac
     2641 cgcggttcag catgtcggtc atgcgcttgt gggctgcgtc gcgacctgtg agcagcttgc
     2701 ctcgcaacag cagcacttcg cccggcttcc aggttgcggc ttgctcacgc gtaacggtgt
     2761 ccaggttcac gcgccgtgcc gatgaaacgt cgtaggtcag ttgaggccag tcctggagcg
     2821 acggcgggtc gagcgccacc ggccctgagc catcgagcac gaagtgcgca tgtcgcgttg
     2881 ctgcacaatt cgggatcatc gccaccggca gatttgccgc atgcgttgga tagtcgagaa
     2941 tcttcacgtc cagcacggtg gtgagcccgc ccaggccctg cgccccgatg cccaactggt
     3001 tcaccttctc gtaaatctcg atgcgcatct cttcgagcct ggtcttcggg ccgcgatcaa
     3061 gcaattcgcg catatcgatc ggctccatca gcgcttcctt tgccagcagc atcgccttct
     3121 ccgccgtgcc gccgatgccg atgcccagca ttcccggcgg acaccacccg gcgcccatgg
     3181 tgggcacggt tttcagaacc cactccacga ttgagtcgga ggggttcagc atcgcgaact
     3241 tcgacttcgc ctcgctgccg ccacccttgg ccgcgaccgt gacatccacc tcgccgcctt
     3301 tgaccatctc gatactgacg acggctggag tattgtccgt ggtgtttttc cgcgcgccgg
     3361 cgggatcggc cagaatcgat gcgcgcagcg tgttgtcagg attcctgtaa gccctccgga
     3421 caccttcgtc agccatctcc tgcaccgtca tggtcgcgtc ccagcgcacc tgcatgccga
     3481 tcttcaggaa aacggtcacg atgcccgtat cctggcaaat gggccggtga ccttcagcgg
     3541 acatgcgaga gttgaccagg atctgcgcca tcgcgtcctt cgccgccagc gactgttcgc
     3601 gttcatatgc gcgcgccaga ctggtgatgt agtccacggg atggtagtag ctgatgtact
     3661 gaagtgcgtc cgcgacgctc tgaatgaagt cttcctgccg tatcgctgcc atcgtgaatc
     3721 cgccctgaac tcagggtaat tgacccgggc gtttcggtct cgggtccgag cgtgccgacc
     3781 cgcctcgaga cgccccaata cggacgcaaa tcaccgccca tttccgaaca atcgtcccca
     3841 attccggaca tcgcgggtct cttcgcgggt caggaccgtc tcagaaatca acttcctgcg
     3901 gcatcccgct gctctcagga accggcgcta caattgctga tcaaaactcg gagatcatat
     3961 gaattctctg acccaggacc ttcgttacgc cctgcgacaa ctcagaaaat ctccagtctt
     4021 cgccctcacg gcggtcatta ccctgggtct gggcattggc gttaatgccg ctatgttttc
     4081 cgtgatcgaa caggttctgc tgcggcctct gccttatcac agcgcctccc aactggtcat
     4141 gattgcgcct caccccgtca ccggcggagg cttcgaccct gcttctttgc cggacgtacg
     4201 cgactggaag acccgagccc acgtgttctc tgatctggcc tggtggacga ttcaggtccc
     4261 aacgctcggc ggccgtgaca atccggaact ggtggcgcaa atcagctcca gcgccaacct
     4321 gctcgaggtt ctcggcgtgc agcctgcaat cggccgcggt tttgttccgg aagatgcgaa
     4381 gcagaatcga gcacaggtgg tgatcctggg cgactcgaca tggcacaaag tcttccatgc
     4441 cgaccgctcc gtcatcggac gcgtcgttcc catcaacggg gatccgtaca ccgttatcgg
     4501 cgtcatgccg ccgaatttcg agttccccca gaccacgggc gatgagattt actcgccctt
     4561 gccgaacgat tcttcattcg aggatcgcgg caactcctca atctcggcgg tggctcgtct
     4621 gcggcctggt atctcggtcc agcaggcaca gcaggagatc aacggaatcc acgcacagct
     4681 gctgcacgag tacgccaatg agtcgaccga gccggtgcgc gtggaatcgt tgcgcgacgt
     4741 agctaccgac aatgtccgct ccggactcta cgccctcgat gcagccgtcg ctgttgtttg
     4801 gttgatcgcc tgtgcaaacc ttgccggcct gatgctggcc cgcgctaacg cccggaggcg
     4861 cgaactggcg atccgaggtg ccctgggggc agccaaatca cgcctcgtcc ggcaattcct
     4921 tacggaaagc ctgctcgtca gcctggtcgg cggagcggcc ggcctggtcg ctgcctgggt
     4981 ttcactgcgt atccttagcc actacctgtc caatgctgtt ccatttggcg agcacatcca
     5041 tatcgacgcc ggcgtctgcg cgattctgat tctcttgtcg tgcctgtcgg cggtcttctt
     5101 tggtgtcggc cctggtctgt acggcgcgaa cctgcccgtc caggagggtc tccgcgatgg
     5161 tgctgcggcc tcggggacca gcaaaaagca cgcgcgctgg cgcgatgcgc tggtggtggg
     5221 tgaaatcggt ctgacgctga cgctgctgat cgccgccggc ctgctgatcc aaacgctctg
     5281 gcagctgcgc catacgaacc tcggttttgc cccggagcag gtcgacgcga cttcgatttt
     5341 catgcctacg catggcgcct ggtggacggg cggggacaag acggctccca gcatcgttac
     5401 cggcttctac gacccgctgc aggagaagtt acgccacacc cctggaatcc agtctgtcgg
     5461 catggctacg atccggccat tcacgtcgaa ccacttcacc ctggacatct ggcctgcgga
     5521 tcgcccgcag ccttcgaagg ccgatgcaat gggcgccgct gtgcgcgcca gcaatgctga
     5581 ttttttccgc accatgggaa ttgcgatcac cagcgggcgc ggtttcgaag agacggatca
     5641 cgccggcgcg ccgctcgccg tggtcgtcaa ccaggaattt gttcgccgca tcctcaaggg
     5701 ccgcgatcct ctgggtgtcc ggctcaaatg ggatgacgaa gacaaggccc tcacagccac
     5761 catcgttggc accatcgccg atcttcacca ggaatccgtg cgggcgggat cggccccgga
     5821 gatctacttc aaccttgagc agctcgtgcc cggacaagac atctactcca tcctcgccgg
     5881 attccacatg gatattgtcg tacgcacgaa tctggcttct gaagtagctt tccgcgccgt
     5941 gcgggatgcc gtgcacgaac tgaacccgaa cctggctttt gccgaacaga ccacgatgca
     6001 gcaggtgatc gacaattccc tcggcgcgca aaccctggcc gcccgtttgc tcgctatctt
     6061 cggcttcgtc gcgctgctga tcgcgattgc cggcatctac ggcctactcg cctattcggt
     6121 cagccagcgc acccgcgagc ttggcctgcg catcgctttg ggtgcgcagc gcgagaacat
     6181 tctttggctc gttttgcgtc acgcgattct cctgcttgcg acggggatgg ctatcggtct
     6241 cggcgtggcc tgggcggcca gcagcgttct gcgctcgttt ctttatgggg ccgctgcgta
     6301 tgatgccgcc acggtcgtcc tggtcgttct gatcctcggc ctttgctcca tggccgccag
     6361 ctatctgccc gcgcgacgcg ccgcagcggt tgaccccatc gaagcactgc gtacggaata
     6421 gcgcttttgt agcgaatact gttacagaat cggggagaaa gcacgatgtt caccgccacc
     6481 caggattttc acttcgcgct ccgccaactc cgcaagtccc ccggatacgc cgctgtcgcc
     6541 atcgtcaccc tcgccctggg tatcggcgcg aacacggcta tcttcctgct gacgtattcc
     6601 atcctcctca ggagcctgcc ggtaccccat ccgagcgaac tgatccgcta taccttccgc
     6661 aaaggcgaga gcgatatcgg gctctcctat ccccaatatc aggcgttgca gaagcggcag
     6721 ggcatcgcca ccggactgtt cgcctggcaa tcgggtgaag cgactttgac ccgcaacggc
     6781 cattcgcaaa agatccccgt cggcctgacc accgggtccg ccttcccgat tttgcaactg
     6841 catccggcgc tgggccgggg cttcgcgccc aacgttggag agaagggcaa cggctggcag
     6901 ccggaggcgc tgcttagcta cgactattgg cggtccgcat tccatgccga ttccgctatt
     6961 gttggccagt ccatccgtct ggagaacacc agcattacga tcattggcgt tcttcctcgc
     7021 ggcttcgacg gtatcttccc cgagcggccc atcgatgttc tgctcccgct cgacttcgag
     7081 ggcatcctcc atccaaaaaa ctccatgctg tatcagcccg gtgcattctg gcttacggtg
     7141 atgggccgtc ttcgctcggg cgagacgatg caggcggcgc aggccaatct ggccgcaatc
     7201 caggcaccgg tgacagaagc cgccgatccc tcgcacaact tcctcactac agggttcttt
     7261 gccgacttca ggttgggggt tgaatccgga cgcggggggc gttcttttct gcgctggaga
     7321 tatgcaaagc cactgctggc gctggaagcg ctctgcggcc tgatgatgct gctgtgtggc
     7381 gtgaatgttg cgctggttgt cgtctcgcgc gtgagttctc ggctgcatga attcgcgatg
     7441 cggagtgctc ttggcgcatc gcgtcgtcgc ctgctcctgc aggttttgac ggagactctt
     7501 ctgctgggtg cgggtggtct ggcgggtgga gctctcctcg gatgggagat ggcgcgcggc
     7561 ctcgtcagca tgatctccgg tccggcaggg ccggacgctg tgcagctgca ggcgggtgcg
     7621 gtggtcttcc tgttcgccgc agtcgtgagc ctcggggcag ctctgatcgc ggggctctgg
     7681 ccggcttggc gcgcctcgca tacggccccc gctctggatc tgaaacagac ggcctccgcc
     7741 cgcaccgcac atcggctggg gcgctggatc atcccttccc aggtcgcact gggggttgtg
     7801 cttctgaatg ccgctctgct gctggccggc acgctctcca gctatttgcg cgaaaactcg
     7861 ggcttcaacg cagatcaaac ttcgctcacg gaaatcgatg tggctaacct cggtgtcccc
     7921 atccccaatt ccgccgcgag agccatggac cttctccgcg aggtcgaggg tgcaccggga
     7981 attcgttccg cagcgctgat gtccctggct ccgattaacg gcggtttttc cgtcggcgga
     8041 tactacacgc tcgatccgaa gggaaacagg atcagcaatc agcaggtttg gcccgagacc
     8101 gcttccacaa gctacttcga tgtgatgggc acgcgtatcc ttgaaggacg gggattccag
     8161 gcttcggatg catctggtga atccatctgc gtgatcagcg ccgcggcggc gacctttttc
     8221 tttcccgggc gatcggccct cggcggtttg ctcaattcgg gggacggcag cgagaagccc
     8281 gttgaccgcg caacctgccg cgttgtcggt attgcggaag atgctcgcat gcgcagtctg
     8341 ctcgagcccc cgcccctcgc tgtctacctg cctctccgcg acgcaaagga cttctcactg
     8401 acctatgcca cgctcggagt tcgcgcagcg aatcctgcgc tggcaacggc agcgatccgc
     8461 caggctttca cgcgcgtctt cccgggagcc cccgcacccc gtatttggcg cttccaggat
     8521 gctgagcgtt acgacctcag ccgccagcgt ctgctctcca gcgtctccgg cggattcgcg
     8581 ctgctcgctc tggccctggt tgcgaccggg ctctacggga tcctgtcgcg ggcagtcacg
     8641 gaacgtcgcc gcgagatcgg catccggatg gcgctcggtg cgcgccgcca gcaaattgtt
     8701 tccaccctgg cgagaggaac cgcgctgcgt attctcgtcg gagtcattgc cggaatcggc
     8761 ctcgctgctg ccgccggccg tctcctccag tcattgctgt acggcgtcac cccgggaagc
     8821 cccctggtcg cgctggccac ttttgcggtg ctgatggctg ttctggtgct ggcgtttgtc
     8881 tttcccgcag ggcgagcggc gtctgtcgat cccatggagg cgat
//
LOCUS       DASZYN010000245         5593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_74084, whole genome shotgun sequence.
ACCESSION   DASZYN010000245 DASZYN010000000
VERSION     DASZYN010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5593
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74084"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..613
                     /locus_tag="VHE33_06225"
     CDS             335..613
                     /locus_tag="VHE33_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97085.1"
                     /translation="MLRLDTLSCALTVLSTVLVGRKMWGGLVVAGVNSVVICLIGIRT
                     QQFGFIPANLFCIGVYAVNLRSWRRGQGSGRRAQGSGSPEQSPEFSQS"
     gene            complement(610..993)
                     /locus_tag="VHE33_06230"
     CDS             complement(610..993)
                     /locus_tag="VHE33_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97086.1"
                     /translation="MRLVVSALFVGLVLPAAAQISSPVLSTAEAAQHVGENRTVCGQI
                     VNTHIAETSHGTPTFFDLDQAWPNQVFDLVVWGDKKADIGNFPKSGKICASGKIVLYK
                     GHPEIFLEDWHSWYVPTGTERASAP"
     gene            complement(1216..3771)
                     /locus_tag="VHE33_06235"
     CDS             complement(1216..3771)
                     /locus_tag="VHE33_06235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT97087.1"
                     /translation="MQTLRQDLAYALRQMRLSPVFTLTAMLTLALGIGATTAIFSLIN
                     TVMLKSLPVADPSSLYRIGAGNDCCIEGGTQDNWGFFSYDFYRQMQANTPEFSSLAAF
                     QAGGFTFGVRRGQSDRVPKPLRMEMVTGNYFSTFGVGAFIGRTLLPSDDQPNSPPAAM
                     LSFRTWQQQYGSDPSIVGSTFIIGDQPVTIVGITPPGYFGETLRANPADLWLPVNQEP
                     NFRKGNSLLHRFQAWLRVIGRVKPGASTATTSQRMTALLRTWLVNDSGMPADWMPGIK
                     ANLPKQNISVIPAGAGVATMKESYQASLNILLAVCCLVLLIACANIANLLLARGASRR
                     AQTSIRLALGASRKRLMRQTLTESILLSIFGGVAGLLVAFLGVKLIVALAFHSAKFVP
                     IAATPSLPVLAFSFALSLVTGILFGTAPAWLATHADPAEALRGANRSTRDHASWSQKT
                     LVVVQATLSVVLLSGAGLLTRSLTNLQHQDFGYQLDNRITLGVTAPWSSYSQPQLDAM
                     YRELQDRLSHIPGVERAALAQYTPLTDNWGEGVIRQGHPMPGPNENSGSSWDHVAPGY
                     LETLGQQIIRGRSISEDDTDATQRIAVVNEAFVQRFFKPGENPIGQHFGLDLPRYSSM
                     FEIVGVVRNAKYVDPANTEPPRPLFFVPLAQHSKYDDALMQSIDDSTHYIEGAVLQVH
                     GNIEGLEPQVRRVFSDVDPNLTLLGLYPLRDTVDSRFDQQRTVASMTGLFGVLALILA
                     AIGLYGVTAYTVERRTSEIGVRMALGADRPGIVRLVLRGAFLQILLGLLIGIPASIGC
                     ARLISTQLYQVKGWDPVVLTAAIVTLSVCALIASIIPARRAASIDPVRALRTE"
     gene            3925..4812
                     /locus_tag="VHE33_06240"
     CDS             3925..4812
                     /locus_tag="VHE33_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HVT97088.1"
                     /translation="MVKRWGWVVVLGILGSVAAAQDLPPAPAPGAAPTVQGLPPEPDR
                     RLCDLNTLLVCAKHVAEDEKGILLSPLRAPAENLWWIVPFGVATGVSVHFDTDAIRDL
                     GVNPSREDKFNKLSDYAGLYGPFAAAGAGYVAGRVKHDDYLKETAILSAEAMADATIL
                     DQGMKYALDREYPLVDNARGGLWPGGPKSWPNHPSMPSEHTMNVWAFAHVVAGQYNGI
                     ATQALVYGAATTVSVSRVLSREHFPSDVVVGSTLGWLIGGYVLHHRSLEHGTLLDVST
                     VETPLGSGVQVRIRLGRGE"
     gene            complement(5229..>5593)
                     /locus_tag="VHE33_06245"
     CDS             complement(5229..>5593)
                     /locus_tag="VHE33_06245"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HVT97089.1"
                     /translation="SAELHFEGDSLEVLSGFPDDVKGSFGFSLRQLQIGREPTSQTRS
                     MSSIGHGVYELKEADERAWYRVIYLSKIENRIYVLHCFEKESRKTDRRDIRIASQRLK
                     LVRQRLQEQKSHDKRSGK"
BASE COUNT         1069 a         1390 c         2109 g         1025 t
ORIGIN      
        1 tcgacgcgga ggccatccgc gcattgctgc gcgcggagcg cagcgggaac gatgaggaag
       61 agtgcgaggg cgagcgatgc gatgcagtat ttgcggaatg gggagtggtt cgtcatcctg
      121 cattcatgct gcgcgaggct gcagcaggaa tgcagtgccg attgtcagga aacggaaatg
      181 acaatcgtca tgcgggtttg gacacacgta tcgtgtgaca accagggggt gacattcgat
      241 acgcagtttg ggggagggcg gttcccggtt gtgcgatgga tcaccgcacg ggccgcagct
      301 acccttcaca ctcggggagg aggatgggcc aacgatgtta cggctggata cgctgtcgtg
      361 cgcgctgacg gttctgtcga cggtgctggt aggaagaaag atgtggggcg ggctggtggt
      421 ggccggcgtg aacagtgtgg tgatctgcct gatcggcatc cggacacagc agttcgggtt
      481 catcccggcg aacctgttct gcatcggggt gtacgccgtg aatttgcggt cgtggagacg
      541 agggcagggc tcaggtcgca gggcgcaggg ctcagggtcc cccgagcagt cgccggaatt
      601 ctcgcagagt taaggggcgg aggcgcgttc ggtgcctgtc gggacgtacc agctgtgcca
      661 gtcctcgagg aagatctccg gatgcccctt gtagaggaca attttgccgc tggcgcaaat
      721 cttgccggat ttggggaaat tgccgatgtc ggctttcttg tcgccccaga cgacgaggtc
      781 aaaaacctgg ttgggccagg cctgatcgag gtcgaagaac gtcggcgtgc cgtggctggt
      841 ttcggcgatg tgcgtgttga cgatttgccc gcaaacggtg cggttctcac cgacatgctg
      901 cgcggcttcc gccgtggaga gcacgggcga ggaaatctgg gcggcggctg gcagaaccag
      961 cccgacgaat aaggcactga ccacgagacg catgctgtcg actccgctca ccggagttcc
     1021 aggcagaaag taaagcagaa ttgcaaaggc cgtatcgact cgacagcagc ggggaggaag
     1081 ttagaacgag agtcgtcgag acatttcggg tttgctgagt atcttgcgat gagagcccgt
     1141 ggaaaggcaa gtgaatttcg tgcggagggg gctcgtccca ggcctcaagc cgggcccggg
     1201 accgaaaatt cccgtctact ccgtgcgcag ggcgcggacc ggatcgatcg aggccgcacg
     1261 ccgtgccgga atgatgctgg cgatcagggc gcagacgctc agggtaacga tggcggccgt
     1321 caggaccacc gggtcccatc ctttgacctg atagagctgg gtggagatga gtcgagcgca
     1381 gccgatcgac gccggaatgc cgatgaggag cccgaggagg atctgaagga aggctccgcg
     1441 gaggacgagg cggacgatgc cgggccggtc ggcgccgagg gccatgcgga cgccgatttc
     1501 gctggtgcgg cgctcgacgg tataggcggt gacgccgtag aggccaatgg ctgccaggat
     1561 gagggcgagg acgccaaaga ggccggtcat gctggcgacg gtacgctgct gatcgaagcg
     1621 cgagtcgacg gtgtcgcgca gcggatagag gccgagaagg gtgaggttgg gatcgacgtc
     1681 ggagaagacg cggcggacct gcggctcgag gccctcgata ttcccgtgaa cctggaggac
     1741 ggcgccttcg atgtagtgcg tggagtcgtc gatgctttgc atcagggcgt cgtcgtattt
     1801 gctgtgctgc gcgaggggga cgaagaagag cgggcgcggc ggttcggtgt tggccgggtc
     1861 gacgtatttg gcgttgcgaa cgacgccgac gatttcgaac atgctggaat agcgagggag
     1921 atcgaggccg aagtgctggc cgatggggtt ttcgccgggc ttgaagaatc gttggacgaa
     1981 ggcttcgttg acgacggcga tgcgttgggt ggcgtcggta tcgtcttcgg agatggagcg
     2041 gccgcgaatg atctgctggc cgagggtttc gaggtagccg ggcgcgacat gatcccagga
     2101 ggagccggag ttttcgttgg ggccgggcat ggggtggccc tggcggatga cgccttcacc
     2161 ccagttgtca gtgaggggcg tgtactgggc aagtgcggcg cgctcgacgc cggggatgtg
     2221 ggagaggcga tcctgtaact cgcggtacat ggcgtcgagc tgcggctggg agtaagaaga
     2281 ccagggcgcg gtgacaccaa gggtgatgcg gttatcgagc tgatagccga agtcctgatg
     2341 ctggaggttg gtgagagagc gggtgagcag gccggctccg ctgagaagca cgacggagag
     2401 cgtggcctgg acgacgacga gggtcttctg ggaccaggaa gcatgatcgc gggtgctgcg
     2461 gttggctccg cggagagctt cggcggggtc ggcgtgcgtg gcgagccacg cgggggcggt
     2521 tccaaagagg atgccggtga cgagcgagag ggcgaagctg aacgcaagaa cgggcaacga
     2581 aggggtggcc gcgatgggca cgaatttggc gctgtggaag gcgagcgcga cgatgagctt
     2641 gacgccgagg aaggcgacga ggagcccggc cacaccgcca aagatggaga ggagaatgct
     2701 ttcggtgagc gtctggcgca tcaggcgttt gcgggaggcg ccaagagcca gacggatgga
     2761 agtttgcgca cgacgcgagg ctccacgggc gaggaggagg ttggcgatgt tggcgcaggc
     2821 gatgagcagg acgaggcagc agacggccag gaggatgtta agggaggcct gatagctctc
     2881 cttcatggtt gccacacccg cgccggcggg aatgacgctg atgttctgtt tggggagatt
     2941 ggctttgatc ccgggcatcc agtcggcggg catgccgctg tcgttgacca gccaggtgcg
     3001 gaggagcgcg gtcatgcgct ggctggtggt ggcggtggaa gcgccgggct tgacccggcc
     3061 gatgacgcgg agccaggcct ggaagcggtg gaggagcgaa ttgcctttgc ggaaattggg
     3121 ctcctgattg accggcagcc agaggtcggc aggattggcg cgcagggttt cgccgaagta
     3181 gccgggggga gtgatgccaa cgatggtgac gggttgatcg ccaatgatga aggtagagcc
     3241 gacgatggag ggatcggatc catattgctg ctgccaggtg cggaagctga gcatggcagc
     3301 ggggggcgag ttgggctgat cgtcggaagg caggagggtg cgcccgatga aagcgccgac
     3361 accgaaggta gagaagtagt tgccggtgac catctccatg cgcagcggtt tgggaacgcg
     3421 atcggactgg ccgcggcgaa cgccgaaggt gaagccgccg gcctggaagg cggcgagcga
     3481 agagaactcg ggggtattgg cctgcatctg gcggtagaag tcgtaggaga agaagcccca
     3541 gttgtcctgg gttccgcctt cgatgcagca gtcgttgccg gcgccgatgc ggtagaggct
     3601 cgacgggtcg gcgacgggga gagacttgag catgacggtg ttgatgaggg agaagatggc
     3661 cgtcgtggcg ccgatgccga gggcgagcgt cagcatcgcg gtgagagtaa agacggggga
     3721 caggcgcatc tggcgcaggg cgtaggcgag gtcctggcgg agagtctgca agggtcagct
     3781 cctctgggcg gatacgcggg gagaggcggc gtggttccgg aaatgtgacg gaatgtaaca
     3841 gaaagggcac aggtcgcagc attcaggtcg cagcgttcag gcgaaggtta caaacccgag
     3901 aaattgcttt cgtatgatgg gcacatggtg aagcggtggg gatgggtagt ggtactggga
     3961 atcctgggga gtgtggcggc ggcgcaggat ctgccgccgg cgccggcgcc gggcgcggct
     4021 ccgacggtgc agggtttgcc gccggagccg gatcgacggc tatgcgatct gaatacactc
     4081 ctggtgtgtg cgaagcatgt ggcggaggat gaaaaaggaa ttctgctgag tccgctgcgc
     4141 gcgcctgcgg aaaatttgtg gtggattgtg ccctttgggg tcgctaccgg cgtgtcggtt
     4201 cacttcgaca cggatgccat ccgggatctc ggcgtgaacc cgtcccgcga agacaaattc
     4261 aacaagctgt cggactacgc ggggctgtac gggccgttcg cggctgcagg ggcgggttac
     4321 gtcgcgggac gagtgaagca cgacgattat ctgaaggaga cagcaatcct gtctgcggaa
     4381 gcgatggcgg acgcgacgat tctggatcag gggatgaagt atgccctgga ccgagagtat
     4441 ccgctggtgg acaatgcgcg gggaggattg tggccgggtg ggccgaagag ctggccgaac
     4501 catccgtcga tgccctccga acacacgatg aacgtttggg cgttcgcgca tgtggtggcg
     4561 gggcaataca acgggatcgc gacgcaggcg ctggtctatg gcgcggcgac gacggtgtcg
     4621 gtgtcgcgcg tgctgtcgcg ggagcatttt ccgtccgatg tcgtggtggg gagcacactc
     4681 ggctggctga tcggaggcta tgtgctgcat caccgatcgc tggagcatgg gacgctcctg
     4741 gatgtatcga cggtagagac gccgctgggg agcggagtgc aggtgcggat tcggctgggg
     4801 cggggggagt gagggccgaa aggtcagatt cctgtccgca tccagccaac ttcgagagcc
     4861 gcgagggaag ctaaaacgca gatgcgagct ttcgcgcagc gctgtgcgta gttgactttc
     4921 cccgacttgc tgtgcggcag ctgcggccct cgatgggacg gacggcgatg cttgtccgga
     4981 ggtggagccg gtctgcatat cgcagcagct tttcgatgct cacctgggag attctgcctc
     5041 tgagcagatt gctcaccgac ggctggtact cgtccaaaat gtccgcgagc tgcgcttgtg
     5101 agtagccctt cactttcaca tgttccagga tggcggacaa gagttcagcc ttgatcctga
     5161 gtgcgctcgc ctgagacgca ggaaatccca gggcatcgaa gacattgccc ttcacgatat
     5221 gtgagggcct attttccgct gcgtttgtca tggctcttct gctcctgtaa tcgctgtctg
     5281 acaagcttga ggcgctgact cgcaatccgg atgtctcgcc gatccgtttt acggctttcc
     5341 ttctcaaaac agtgcaatac gtagatcctg ttttcgatct tcgaaaggta gatcaccctg
     5401 taccacgccc gttcgtcagc ttctttcaat tcgtagacgc catggccgat ggaactcatg
     5461 ctgcgagtct gcgaagtcgg ctctctgccg atttgtagct gccggagcga gaatccaaac
     5521 gaccctttaa cgtcatcagg aaacccggac aacacctcca gcgaatcacc ctcaaaatgg
     5581 agttcggcgg atg
//
LOCUS       DASZYN010000246        38848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1230548, whole genome shotgun sequence.
ACCESSION   DASZYN010000246 DASZYN010000000
VERSION     DASZYN010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 38848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38848
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230548"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1407)
                     /locus_tag="VHE33_06250"
     CDS             complement(<1..1407)
                     /locus_tag="VHE33_06250"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVT97090.1"
                     /translation="MSSRILRRILLSLAILPVVLALPCLPARAQSGTQGKVVVTVQDT
                     SGAVVPGTTLRLVEHQTNDTYTASTNSSGAYTFVNLPIGTYTLTISRSGYANKVYSEV
                     IVQASQVTDLTAQLTVGATTQTVQVAGQTSPLLQTTSNAIGSVVDMKQIQNLPLSGRD
                     LTSLASIVPGFTGAGGQGTFNGLPIEDEGSNMDGMVGSATRMKFDANLAPAVSPRLED
                     IEQMSVQTDQLGLNSGFGQATTQINFVSRRGGNQFHGSAFEDFRNSGLYANSWHNDAA
                     GVRKGKEIRNDFGVSAGGPVLHDKLFFFGTYAMRKIPGSYAASNNAFTQAAQSGNFTY
                     GNTTVNLLTIAQNHGLPSTVNAEVAKQFSAINTATGSGKLVPTSDPNFNEIDWVQSAP
                     ETDYYPVARIDYNASQKARFYLSWMMTQINQPGNNAPTFPGSSFANQTAGNQTRNFTG
                     SFGFDYIFSPALINQLKAG"
     gene            complement(1687..2808)
                     /locus_tag="VHE33_06255"
     CDS             complement(1687..2808)
                     /locus_tag="VHE33_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT97091.1"
                     /translation="MHVRAIGAALKLIALLIGASSFLSAAQSAGKLQAPPVSRSEPQA
                     LHDEATARAELQTGAQLTANGFLQQAIPHLLAARAAGIAPYAAGVNLGICYLGTGRFK
                     DAIDVLHALDASGRGSAAVENLLAQAYIGNAQPQEALRSFRRAAAATPKDEKLYDYIA
                     DACTDHKNYALGLQIVALGLQQLPNSARLHYEKALFLSQLGRFGEEGKLEFDRAAELA
                     PETYIGYLARVQKNLYEDNLLAADKLLHEAVQSGQRDYRMLSLLGTVLLHEGAAPGEP
                     RFEEARKALEQSAQENASYSATQIALGKVYLMENQPQNAIDHLEIGRRLEPDNSSVYA
                     SLASAYDRLGNHVQARQMRLRMGRLLAEKSGGANATQEP"
     gene            3004..5331
                     /locus_tag="VHE33_06260"
     CDS             3004..5331
                     /locus_tag="VHE33_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97092.1"
                     /translation="MAATTAAFHEFFSEAETSAAPIDQSGLVHDMLRDSDASVEGHTR
                     LSSFTRGKEQWTVYEDLRTRDGAITFVSSEGSARVLTKSAEAVFAEDEAQYLGLNLSE
                     IGMSGPDLLADKLLAKGEPDEDAVRRAAPPMDSAAPEGRYSRPHWNAIVGTRECSDTM
                     PVYPAGSTRTWHPVQYFPEITNERARKRQEGLVGGWMPAVRKLFPGDDGSVFEAIVFG
                     DVLAHDRFIVQTWHRVAQIRDGKVVKVVYGYSYPAYTRRRQNPDATEFYLGLLEFAEY
                     WDRQMHDVAAVSLPDASWANMAKHAFVKEQIVRPGGVYPKYGAVDRDYYGPEYDGFQD
                     IFTMSLYANLECGRFAQARDVLDDYFSRFVNAKGMINMRGPETAQFGLTLSLIARYFR
                     YTGDTSLLRKHREKIEATASLLTELHDEAMQLPSGDRGFGLIHGWSESDSCLMEDPSI
                     WWKPYFSNSAFTVRGWRDLAEMWKDVAGPGGEENSSDWMRRSRELHVTLVKSIDANIL
                     RDRKPPYLGPLPGTKLTFWESMQQEHPSEQGWSHRAYAELLQAGVLPDVQTAVVIDCM
                     RAYGATTLGVVANVERPHPQGRDILGFISYGYAQALLTLDRIDEYLLFLYSHRYHDHS
                     RGSWMAGEVSGITGDGALFCIPAQQTIPLLLRWMLVFEDPDGQRLDFGRALPRHWIGT
                     KKPIAIEQAPTRWGRVDYRLESHGDAQLIATIGLPKSGPLPPTIQVTFRVPQGRSLQG
                     LSVNGQSQPLGGKNMDAAVFAPGGKRDFSVIATLT"
     gene            5442..7418
                     /locus_tag="VHE33_06265"
     CDS             5442..7418
                     /locus_tag="VHE33_06265"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HVT97093.1"
                     /translation="MSSAAYAGAAALLPHGAMGLASQHTQKVAAATGEDTAVQYLTNA
                     AVAKVQWKAKPFPMPEVRLLPGFWKDTMELNRSFLYSLPNERLAYNFRVTAGIPTDAD
                     PLGGWEAPDCELRGHYVGHYLSSCALLHASTGDEFIAAKGNELVSILAECQAKDGYLG
                     AYPATLYEKLRKHERVWAPFYTYHKIMAGLIDMYQHTGNQQALEMATRMADWAHTYAM
                     SFTDDDWQRVLLVEQGGMNEASFNLYAITSNTKYRDLGFRFEHHKIFDPLAANQDALA
                     GNHTNTNIPKVIGAARGYELTGDGRYRQISENFYRMVTEHHAYCTGGTSNGEFWQAPD
                     AIATQLGPAAEECCCSYNMMKLARHLFGQNPDPKFFDYYERLLLNVRYGTQDRNGMLM
                     YYVPLQPGMYKTFGTEFDSFWCCTGTGSEEYAKLTNSIYFHDDNAVYVNLFMPSALDW
                     KERGLKLEQTTKFPDDERVTLTVKSAPSATTALKIRVPYWATQGVAYSINGQPQNVTA
                     TPSTWVTLEHDWKAGDVITIDMPLTLHLAPTPDDKQVQAAMYGPLVLAAVLGTEGLTT
                     GMIYGGSGPGGNDDGYPMPTVDMRPHRHRGPDGKPVEGPPPEPGAIWFERAEGSRRYP
                     LQFRTKGRGPTHTLVPLNQIMDERYSVYLRNLTA"
     gene            7518..9227
                     /locus_tag="VHE33_06270"
     CDS             7518..9227
                     /locus_tag="VHE33_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HVT97094.1"
                     /translation="MVIRIAAVLTVVVSLVVAALANGLGQSASGNDRLPADFEDVTAP
                     LGVHFIHQAPHSSRKYLLETMGSGVALFDYDNDGRLDLYLVNGAPFDDFVPKGTIPEK
                     TDPKYWNRLYHQRADGTFEDVTEKAGVAGVGYGMGVAAGDYNNDGNEDLFVTSLGENH
                     LYRNNGNGTFTDVTAVAGVGGSGWSTSAAWVDLDGDGYLDLIVLRYIRWDWDDVWCGP
                     KDNRGYCHPDAFPPISMLVYHNNGNGTFTEQAHKSGLDVPGKALGISIADYDRDGHPD
                     IFVANDSMQEFLFHNTGAGKFDQVGLESGIAVDGDGRTFAGMGTDFADYDNDGWPDIF
                     VDDLANQKYALFHNDRDSTFSYDSFVTGIGGLTLLHSGWGARFMDYDNDGWKDLLIAQ
                     AHDLDTIEKDFPQLHFREPPLLLRNVDGRKFVDVGPVSNIVFRERWVGRGLATGDIDN
                     DGRIDAVITENGGPVHILMNRTKSTNHWIGFKLVGHRSNRDGIGAVIRIDTATGSQWV
                     TVTTSSSYMSASDERAHFGLGRESTVQKVEIRWPSGIRQILTTVAGDRYIRVEEPAAS
                     AGK"
     gene            9600..10472
                     /locus_tag="VHE33_06275"
     CDS             9600..10472
                     /locus_tag="VHE33_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT97095.1"
                     /translation="MESTVIAERTAPAAEAPFSLAGRTALVTGAGRGIGRAIAKRLAS
                     AGARVMVNDLDEVMLRESEAELACPERVRHVQGDLTDPATPAAIVQATLDAFGTIDIV
                     VNNAGYSWDNVIQKTTDEQFLAMLDIHLVTPFRILRAASTFIREAAKKEIAAGKMVMR
                     KVVNITSIAGTDGNSGQAGYSSGKAGVIGLTKTLAKEWGRYNVNVNAVGFGLIQTRLV
                     QDLNAPGASMEMHGHQIRLGVQPTLLESVKSACPLGRLGTPEEAAGTVLFFCSPLSDY
                     VTGEVLICGGGLHF"
     gene            complement(10821..12434)
                     /gene="aceB"
                     /locus_tag="VHE33_06280"
     CDS             complement(10821..12434)
                     /gene="aceB"
                     /locus_tag="VHE33_06280"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HVT97096.1"
                     /translation="MDSSLEKIVTTALESNTDGWSSEERDQEILTPEALQFLRELDFR
                     FEARRVQLLEARRRRQEEFDRGCLPKFLAETDDIRHGDWVCAPIPIELLDRRVEITGP
                     PDRKMVINALNSGANVFMADFEDSNAPTWKNCIDGQRNLRDANLRTISWCSSEGRIYE
                     LGRNPAVLFMRPRGWHMVEKHLCFSGAPVSASLFDFGLYFFHNYRTLLKRGSAPYFYL
                     PKLESRLEARLWNDVFLFAQEYVGIPKGTLRATVLIETIAAAFQMDEILYELRQHSAG
                     LNCGRWDYIFSFIKRFRGRAEFVLPDRGTVTMEQPFLRSYVELLIHTCHRRGAHAMGG
                     MAAQIPIRNDEKANEEAMERVRQDKLREVRAGHDGTWVAHPGLVAIAREVFDRYMPEK
                     NQIRIPAEKQSRIASTDLLQVPEGPITEAGLQRNIDVALQYIESWLRGNGCVPIYNLM
                     EDAATAEISRAQLWQWIHHGAHLQDGRIVTFDLVESVLKEVLDQMRSMMGAKAWAASR
                     FTSASEILKELSTGEFQEFLTTAAYSELD"
     gene            complement(12472..13785)
                     /gene="aceA"
                     /locus_tag="VHE33_06285"
     CDS             complement(12472..13785)
                     /gene="aceA"
                     /locus_tag="VHE33_06285"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF011645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HVT97097.1"
                     /translation="MLKSSGNNGFHSPRWTGISRAYSADEVKRLRGTVYVEHSLARSG
                     AQKLWRLLHEQKYVAALGALTGNQAVQMAQAGLKAIYLSGWQVAADANLSGQMYPDQS
                     LYPVNSVPALVRGINNALMRADQVAHAEGHENIDWMLPIVADAEAGFGGVLNAFELTK
                     AMIEAGAAAVHFEDQLSSAKKCGHMGGKVLVPTQEAVQKLIAARLAADVLDVPTILIA
                     RTDADAAQLITSDCDATDREFLTGERTVEGFFRFRGGVEAAIARGLAYAPWADMVWCE
                     TSEPNLEEAARFAAGIHSQFPGKLLAYNCSPSFHWEGKLSQAEIAHFQQELARIGYRF
                     QFVTLAGFHALNLSLFELAHDYAHRGMTAYARLQEREFDQAKKHGYSAVKHQRFVGTG
                     YFDRVASTIASGQISTCALPGSTEEEQFDSPADHAPTLSELRAII"
     gene            13980..15656
                     /locus_tag="VHE33_06290"
     CDS             13980..15656
                     /locus_tag="VHE33_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97098.1"
                     /translation="MQTNSPSARLDSHPPDRSSDSDHTPNNTRIGHLAERLSHSLHFR
                     RIAAGKAILDESEPILARLDPGAPQALRVLLLVAQWVDVGYRDHRFLETLLRPFSADL
                     RRRLPVVDYLRLRMVDAFRALAMEDVDAAIAGLDLVLRAESELCDESLAALAHFWKGR
                     AHRRKGEYETSLLHIARARELAEKSHDDVFTAVIQIHESWLLFQKGQRREALRLLTHA
                     EHVLQPTDHYIALGNIESARGRIVRRSGEYTRSLEHFERARELYSQGDPNHLNMARVL
                     VNGAYVRRLLALQLRRKIDRRTWRDRSSSGDGSLRARYQQLSESAIHDLKQARQIYAT
                     HTHTGGIGNVLFNLGYLYLDRGDIDHAAHQAREAYHLGLEKSDHILMARARILQAAAE
                     NARVEEQLGEDVDIAVHANRAREESDEALALARGTQNRRLTAGAWIARGITAANDFFQ
                     DWELARHCATEAAALMAPDENDHLTEDIAALKSKILQVSGISDTLRSWSEGMVGDKTF
                     QQITEEFAEIVIPKVWLRENRKISRVAERLSVSPKKVRRILRNAGLRSRS"
     gene            16146..17333
                     /locus_tag="VHE33_06295"
     CDS             16146..17333
                     /locus_tag="VHE33_06295"
                     /inference="COORDINATES: protein motif:HMM:NF006552.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HVT97099.1"
                     /translation="MTHREVVVLSAVRTAIGKFGGALKDIPPTELAAKVVCQSVQRSG
                     LAPSEIGHVILGNVIHTDQKDMYLARVAALRGGIPVETAAMTVNRLCGSGLQAILNAA
                     QAIAHGDTDAAIGGGVESMSRAPYWLPSMRWGARLNDATAVDALVASVTDPFDNVHMG
                     ITAENVARKWGISREDQDALAVESHRRANEALKSGKFASQIVPIELKTKNGPVFFNTD
                     EQPRSDATLENLAKLKPAFDPAGTVTAGNASSINDGAAAVVLMERSAAEERGLKPLAA
                     LVDYAVVGVEPAVMGIGPVAAVRKLFERTGLSKEQMEVIELNEAFAAQLLAVRRELDF
                     PPERTNPNGSGISLGHPLGATGAVVTVKALHELRRVNGRYALVTMCIGGGQGIAAIFE
                     SMN"
     gene            17353..18135
                     /locus_tag="VHE33_06300"
     CDS             17353..18135
                     /locus_tag="VHE33_06300"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HVT97100.1"
                     /translation="MDTAVLIEPQPSFARPLEGRVALVTGASRGIGRAIARELASRGA
                     IVVINFFNSIERAQQLRDDIESAGGETSLLQGDVSNRQEARRIIHHLLEVYGRIDVLV
                     NNAGITRDRSIRKMTDNEWLEVIETNLNSIFYCTSAAVPSMIEHKFGRIVNIASFSGQ
                     AGNFGQANYAASKGGIIAFTKVLALELARFNITANVVAPGFTSTDMLAAVPGDILDQI
                     KTRIPLGRFAQPEEVAKAAAFLICDGDYVTGQQININGGIYM"
     gene            complement(18151..19218)
                     /locus_tag="VHE33_06305"
     CDS             complement(18151..19218)
                     /locus_tag="VHE33_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVT97101.1"
                     /translation="MEPAFAMSGAVAGFEKRLRTFNRVLTTRAPIAQTPKDLVWALNK
                     AKLYRYRPTVDPTHQHRLPLLLIFAIMNRPSILDLRPGHSFVEYMVGRGYDVYLLDWG
                     APGYEDRHLKLDDYALEYLPRAIRKVKAIAGVEEFSMLGWCIGAILTTIYAALRPDEG
                     LRNLLLLTAPLDFSNRNSVTFARWTDERYFKVELILDAFGNMPAEMIDSGAKALKPIE
                     NYITNYCKLWDNLDDPRVVESWHAMNTWVTDNIPMAGAAYRQLIVELYRQNRLIRNEL
                     VVRGERVDLGRMRANLLTVIAEGDHITPACQSETLLAKAGSRDKELYRIPGGHIGMMA
                     GSGAPRTTWPHIEKWLSARSG"
     gene            complement(19221..19745)
                     /locus_tag="VHE33_06310"
     CDS             complement(19221..19745)
                     /locus_tag="VHE33_06310"
                     /inference="COORDINATES: protein motif:HMM:NF021246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97102.1"
                     /translation="MLHVSKKEVRLMSETQEKTSKTYDPFEPFRSMRDTYLDAMAKAM
                     VETVNTEAYAQATGAMLEGYLTAIAPFREALDKSMLQTLQQMELPSRQEVAALAERFT
                     NLEMRLDDMDAKLDAIRKHMEQNGSGPQSGVGKAAAATPTPRTGEGGDHETAVSRPGA
                     RQPFVRRKLSRTRR"
     gene            complement(19799..20317)
                     /locus_tag="VHE33_06315"
     CDS             complement(19799..20317)
                     /locus_tag="VHE33_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97103.1"
                     /translation="MDWAESMAKLFTAGVGRIADVQKKGLDIAIQQNAELIDVWKKAI
                     QKVPGTPGLFLLELGASGFDHYAEVQKAAIDLVVEQSKAFADVLKERAATATNANEEV
                     DQFTKKSIERVVAMQKKALDQSAAQAKAVVETSSKQFGEGSPVEMAAGSMQRGVDAIV
                     DAQKELLDMAVR"
     gene            20470..21054
                     /locus_tag="VHE33_06320"
     CDS             20470..21054
                     /locus_tag="VHE33_06320"
                     /inference="COORDINATES: protein motif:HMM:NF019494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HVT97104.1"
                     /translation="MPPKSILIKKYENRRLYDVTNSRYVNLEEVAQLLREGHDVRVVD
                     AASSKDITRLILTQIITEDAKTTDSTFPLDLLRQMILTSGRASQETALRYMKAMLDLY
                     QDTYRAMSPAVGPFGMFRPGFGGPVAGRDQPISMNASGEEPTNRAPQETKQVDELKQR
                     VADLERLVSSLTPSKVAQRKPRRSKPGAKSARLR"
     gene            complement(21287..21374)
                     /locus_tag="VHE33_06325"
     tRNA            complement(21287..21374)
                     /locus_tag="VHE33_06325"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21338..21340),aa:Leu,seq:caa)
     gene            22188..22664
                     /locus_tag="VHE33_06330"
     CDS             22188..22664
                     /locus_tag="VHE33_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97105.1"
                     /translation="MAAKAEAACSGAGGVEPGPAADAGRTPIGTDDPTGMNPAGSGRT
                     DEAGRLAGAEDDRRMEGEAHAEGGGSVAKKLVEGGATHAQASEIGEIGRDTRVGIDER
                     DSGEGEALEIGEHAESSEGAPSVRHKTFAARFIDGSLAGVGDEDIGSTETECDSGR"
     gene            complement(22721..23770)
                     /locus_tag="VHE33_06335"
     CDS             complement(22721..23770)
                     /locus_tag="VHE33_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HVT97106.1"
                     /translation="MHILATATAFPEHYITQREFLDALLPYWGTDAKLTSVIERLHLR
                     TGVDGRYFSRPLTDYLDLDTFGKTNDAWIDTALDLGQRSIDCALKQAGLSPDQLAAIF
                     FVSVTGVASPSIDARLMNRMPLSPHIRRNPIFGLGCVAGAAGLSRAADYVRAYPDQVA
                     VLLSVELCSLTWQRDDKSIANLISVGLFGDGAAAVLVAGDQVQRRGPRIVATDRVFYP
                     NTEEVMGWSVSEKGFNIVLSPDVPKVIVENLGRNVDEFLAVHQLTRSDIGSWIMHTGG
                     PKVLEATQEALGLSRDDLDVSWEALRRVGNLSSASVLVVLDEVIKQRRPPSGTRSILA
                     AMGPGFNAEMLLLEW"
     gene            complement(23886..25367)
                     /locus_tag="VHE33_06340"
     CDS             complement(23886..25367)
                     /locus_tag="VHE33_06340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVT97107.1"
                     /translation="MFSRLARFFYRTAAWRLALQTTLVFAAASASVFLAMYFIVARTV
                     RERSDSWLTGESETLRQVALSTPRDALYSRIVEEVAELATQEFSWDGGTANTGGNTVF
                     FAQIGNTSEPPLWVGPQNSGAFLGLLRTLNIPQHQAVSLRVAGFAEPFRVVAVDVAPG
                     AGHIYLGLLDRGAEHLLRQLLVRFFLGWLFMVAFGFLLTLFGLRRLLNRVDDITGAAA
                     SIRTDDLGTRVPIGTQHDEIARLARTFNSMLDRISASVNQLRTLTDSVAHDLKSPITS
                     VRGSLEIALSLEDREQSQDLIASAIENLDRLSEVVTTSLDLAEADGGALRLRRESLDL
                     SDLVHRVAELYAPAIADAQQHLTVSAPEPVPIVADLSLCTRLLSNLMENELRYAGPGA
                     HISVVVTSRASTGSVAVEDDGPGFPMDLLPRVLQRFVKGSNSQGHGLGLAFVSAVAHA
                     HAGVATASNRATGGARILVEFPLSDSPRVTGDDGLADNLLTSS"
     gene            complement(25371..26078)
                     /locus_tag="VHE33_06345"
     CDS             complement(25371..26078)
                     /locus_tag="VHE33_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT97108.1"
                     /translation="MAALRGHPGVFMRLLVVEDDNTIRDFLTRALAESGFGVDACAQG
                     CEAERLALEGVHDALVVDLNLPDGDGLDLIQRLRSQGVSSPVLILSARRSVDERVLGL
                     ERGGDDYMTKPFAVAELQARLRSLLRRSTPGQSETTRLRVADLEIDLIRREARRAGIE
                     LQLTQQEFALLEYLCRNAGRVVTRAMVLDHVWKMRIDTSTNIVDVHIHRLRNKVDRES
                     QRPLIRTIRGVGYLLRE"
     gene            26398..27525
                     /gene="gcvT"
                     /locus_tag="VHE33_06350"
     CDS             26398..27525
                     /gene="gcvT"
                     /locus_tag="VHE33_06350"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HVT97109.1"
                     /translation="MSTTAALRKTPLNGLHRRLGAKMVDFGGWDMPVEYSGLIAEHMA
                     VRTGVGLFDVSHMGDIQFRGPGSLAAVQHISMNDASRLQVGQAQYSAMLYPEGTFVDD
                     VIVHKFSENDYLFVINAGTREKDYQWVRSQARGFDCHVSDYSDLYTQLAIQGPKAQET
                     LQKLTQTDLSKVKFYWFTWGTVCGLPNTLIARTGYTGEDGFEIYVPSDEATSERVWNE
                     VIEAGKEFGIVPCGLGARNTLRLEAALSLYGHEISESLNVWEAGLDRFCKLEKGEFVG
                     SEALKKVQAAGGPEKRLVGLEVVERGIARDGYRVMDPDGKEIGYVTSGSPAPFLKKNI
                     ALAYVPRSLAEVGTEVAVEIRNNPVKATVVPTPFYRKPKKT"
     gene            27626..28015
                     /gene="gcvH"
                     /locus_tag="VHE33_06355"
     CDS             27626..28015
                     /gene="gcvH"
                     /locus_tag="VHE33_06355"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HVT97110.1"
                     /translation="MAYPANYRYTREHEWIDVSGKTGTVGITDHAQDSLGDIVFVELP
                     KVGDKVEKGKVFGSVESVKAVSDLYSPASGTVTAINEELTTSPEKINTDAHAAWIMKI
                     ELSDAGDVGSLLSAEDYEKYVKEETGH"
     gene            28078..29454
                     /gene="gcvPA"
                     /locus_tag="VHE33_06360"
     CDS             28078..29454
                     /gene="gcvPA"
                     /locus_tag="VHE33_06360"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HVT97111.1"
                     /translation="MRYLPKSDAERAAMLRDIGVSSIDDLFAVIPQEYRLQRDLEIPR
                     QMGESEIVDHFKAAANRNANGYASFLGAGVYRHYRPVIIDTIVQRGEFLTSYTPYQAE
                     ITQGTLQAIFEFQTMICELTGMDIANASMYDGSTACPEAAMMAMRVTGRDGVVVARTV
                     HPEYREVMRTYAQHREKESGLKAQEVGYDPEGTSHAGRIDLAALEAAVTEETACVLVQ
                     SPNFFGVIEDVGAIAEIAHRKGALLVVAIAEAVSLGIVRPPVEADIVIMEAQSYGVAP
                     SFGGPFCGVIAAKEKYVRQMPGRLVGQTTDKQGRRGFVLTLSTREQHIRREKATSNIC
                     TNQALVALMATVFMTVYGKEGMRELALQNLAKADYAAKTLGAQGKLLFDRSPRFNEFV
                     LQTDEAPETLNARLLEQKIIGGVALKNWYPELRNATLWCATELTAKEQIDRAAGVLAA
                     ATAAVGAR"
     gene            29455..30966
                     /gene="gcvPB"
                     /locus_tag="VHE33_06365"
     CDS             29455..30966
                     /gene="gcvPB"
                     /locus_tag="VHE33_06365"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HVT97112.1"
                     /translation="MAAQIEEKFVGTTRKVTGHLTQNEGLSFEKSVPGKKAYRLAPLD
                     VPEVDPSALLGEAQRSESAQLPELSEIEIIRHFTRLSTWNYAIDLGMYPLGSCTMKYN
                     PRVNEFVSRLEGIAEAHPYQPEELSQGSLGILRLLQKCLIEITGMDAITLQPAAGAHG
                     EFTGILLARAYHESKGNPRKRVIIPDSAHGTNPATAAICGYEVVNFKSNPNGGIDVAA
                     LAQAVTEETAALMLTNPSTIGVFENEIHRIAEILHAKGALLYMDGANMNALCGKTRPG
                     DFGVDVMHLNLHKTFSTPHGGGGPGSGPVACRKHLEPFLPVPVVEEGKDGHLRLNYDR
                     PQSVGRVRAFYGNFGMFVRALAYILANGPDGLRQTTEDAVLNANYIRKKLEGLYELPY
                     TTPSMHEVVFSDRRQLRNGVRTGDIAKRLIDYGFHPYTVSFPLIVPGALMIEPTESES
                     REELDLFVDAMQQIAREVEENPQVVLDAPHSTRVSRLDETTAARKPILRWKNS"
     gene            31137..32261
                     /locus_tag="VHE33_06370"
     CDS             31137..32261
                     /locus_tag="VHE33_06370"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HVT97113.1"
                     /translation="MHATRTFPHAMLREIYEQPEALAATMARYAPGGTLDTNIFGPVA
                     EALRGRQRVVIAASGSSRHAGLAGEIMLEDNAGLAVDVEYSSEYMYRSTHTLHDPGVL
                     VISQSGETADTLASLREAKARGLRTVAIVNDADSTMAREADASLPTMAGREIAVPATK
                     SFTTQLTVLYLLSLFLARIGGRMTAQTVSAHCEQLERLPHLLNQTLEFWEKRVAAIAE
                     KLWEARTFLYLGRAIHYAIAREGALKLKESAYVQAEGLPAGELKHGPNALVTPEAPLV
                     MLATRDHNDPDSTLRYEKTLQLMRDMHAQGAEIAAVHVEGDDEVPELAQHAVAVPAVN
                     EFLLPILEVVPLQMLAYFVAVRRGVNVDAPRNLVKAVVRE"
     gene            32366..33652
                     /locus_tag="VHE33_06375"
     CDS             32366..33652
                     /locus_tag="VHE33_06375"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT97114.1"
                     /translation="MAQPAAAFLSRDFLRYQLARVVVIIGAEAQALAVAWQVYEITHR
                     AIDLGYTGLILFLPGLVFMLPAGHVADRFDRRQVILVCYALQAVATFLLFWLAWTSVG
                     HVWQIFVLLFAIGTGRAFSGPASSALVPHLVPTEHFVNAITWGSAIFQFSNIVGPAVG
                     GVLFTLPPQGRLHGAALVYLFTLAAIVWFLVLVGSLHVRPGRMEHRDISKDVLLAGFH
                     YVARTQVLLGSISLDLFAVLLGGAVALMPIFAQEILHAGPRGLGMLRAAPALGALTVS
                     LWLMVRPIQRRAGVRMLVCVAIFGAATLLFGVSKSLPVSLAALFIVGASDMVSVVIRS
                     SVLQLATPPEMRGRVSAVNSLFVGASNELGEFESGLTAQWWGAVRAVVIGGVGSLVIT
                     GLWAAFFPSLRRADALTPEALLEAEKDHTGREVRQL"
     gene            33740..34777
                     /locus_tag="VHE33_06380"
     CDS             33740..34777
                     /locus_tag="VHE33_06380"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT97115.1"
                     /translation="MAEALVTGGSGFFGGILKRELLARGYSVTNVDMVPDADRHSALN
                     SVQADIRDEAFLDSLFASRRFDVVFHCAAMLAHGSMDEKLLWTSNVGGTQRLADACRK
                     YRVEKFVFTSSNCLWGKNLGHPVREDEAPAPVELYGRSKLAGEQALADYTSDFDVTIL
                     RCPTIISGGRLGLLAILFEFIQEGKTVWVVGSGSNRYQFIYAQDLVNACMMATKTSGS
                     DTFHVGSDNVQSLREVYEAVIEHADSPSRVRSLPKGPTLAAMHLAHVLRISPLGPYHY
                     KMIAEDFLFDTSKIKERLGWQPTLTNAEMLIEAYDYYVERRAEIESRQDVSAHRRAAP
                     MGIIRLVKWIS"
     gene            34774..36093
                     /locus_tag="VHE33_06385"
     CDS             34774..36093
                     /locus_tag="VHE33_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97116.1"
                     /translation="MKSVQVEPGAVAEPSGSQFRFPWSIFWIALGVRLAALTLGHYYR
                     IAPYEDHFAFGWEMGRIARSLATGQGYANPFDHFSGPTAWVTPLYPLILAADFKLFGV
                     YTAVSAWMILAFDSLLNALMVRTTWEIAARCFNRKVAVWSAWIWAVYPAAMQFAVKWV
                     WEISLTAFLFQCILVLALRMRNVGGAPEEAENAASTPRWGAFGLLWGLITLSSASIAI
                     FLPFCGLWILKGAGSLRRQIPRVLLASVVCVACLVPWLVRNALVFHRFIPMRTNFGAE
                     FNMGNNPYLEGTVIGSPVALAHEETLLDKMGEVQYSKMRGREAMERIRREPSVFLALT
                     LRRFYYFWADVPHATIRGKEWTEYARGLNFEFASLAGVMGLILAVRRRVPGAVLFAMA
                     FAVIPFVYYFVFVQARFRHPLEPLITILGVYLFQSAEKSWRVGLPRR"
     gene            complement(36156..36641)
                     /locus_tag="VHE33_06390"
     CDS             complement(36156..36641)
                     /locus_tag="VHE33_06390"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVT97117.1"
                     /translation="MASVILLVAVAVCGCLTNPYEVSMSPQQQQHAISLLRDAYSAFN
                     RNDISAAVHALDLNIDWQEPAEFPGGGSYHGRAEVARYLANSRAGWAEGSSEPVRFIV
                     HGDRVVVYVHARFRTKDSSAWTEVCLADVYTFRNGTPVAMRAFADRNAALEWVGVPEL
                     D"
     gene            complement(36690..37325)
                     /locus_tag="VHE33_06395"
     CDS             complement(36690..37325)
                     /locus_tag="VHE33_06395"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HVT97118.1"
                     /translation="MANLFEQPRFIAVEGPIRAGKSSLARILAETLQARRLVEPENNP
                     FLDRFYRGEPGLAFATQMWFLMERYEQQRELEDFTTPIVSDYLFEKDKIFAYINLSDA
                     ELDLYNRCYQMFRADVPTPELVIYLQASPEALNQRIRRKGVPGERFISEKYIEQVAAA
                     YEHFFFHYSASDLLVVNTSDIDFVNSNRDLQLLLKRLAEPVHGTQYFLPLG"
     gene            37406..38128
                     /locus_tag="VHE33_06400"
     CDS             37406..38128
                     /locus_tag="VHE33_06400"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVT97119.1"
                     /translation="MIGVLASVAGAAGLSAGVWTYGSLAPESQLFGRTIVAGPNAAEF
                     ALTFDDGPNDPWTGRLLDVLARYQVRATFFLIGRFVRQCPKVVREIRDAGHCIGNHTV
                     SHPWLAVQSGARVREELSGCNVALEDVLGEKVQFFRPPHGSRRPAVLDTARELGLTAV
                     MWNAMGFDWRANITAAQITEHLEQGIARNQRRKRGSNLLLHDGGHAALGADRSQSVEA
                     TRNILERHPPGTVRYVTADAWV"
     gene            38211..>38848
                     /locus_tag="VHE33_06405"
     CDS             38211..>38848
                     /locus_tag="VHE33_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT97120.1"
                     /translation="MFEDIRYAVRQFLKTPGFTITAILTLALGIGATTAIFTLINAVL
                     LKSLPVANPSELIRIGDNENCCINGGMQDDWSLFSTEQYRRFRDHTPGFSNLAAFQAG
                     RTQVGVRRAGSDHPAESFGAEFVSGNAFETFGLTPWMGRLLRPSDDVKGAPLVAVMSY
                     RTWQQKFGKDPSVVGTSFFVNGNPFTIVGIGPPGYFGERLTTDPPSLWIPLN"
BASE COUNT         7848 a        11075 c        12219 g         7706 t
ORIGIN      
        1 acccgccttc aactggttga ttagagcggg tgaaaagatg taatcgaatc caaaagaacc
       61 agtaaagttc cttgtctgat ttccagcagt ctgattggca aaactgctgc cgggaaaggt
      121 gggcgcattg tttcccggct gattgatctg agtcatcatc caggaaagat agaatctggc
      181 cttctgtgaa gcgttgtagt cgatgcgcgc gacgggatag tagtccgttt cgggcgcgga
      241 ttgaacccag tcgatctcgt taaagtttgg atcggaggta ggaaccagtt ttcccgagcc
      301 agtggcagtg ttaatggccg agaactgctt tgccacctcc gcattgacgg tagacgggag
      361 accgtgattc tgcgcgatgg tcagtagatt gacggttgtg ttcccgtagg tgaaattgcc
      421 actctgagcg gcctgtgtga aagcattgtt gctcgccgcg tagctacccg ggattttgcg
      481 catcgcatag gtcccgaaga aaaagagttt gtcatgcagg actggcccgc cggcgctgac
      541 gccgaaatcg ttccggattt ccttgccctt gcgtacgccc gcggcatcat tgtgccagct
      601 gttggcgtat agcccgctgt tgcggaagtc ctcaaaagcg cttccatgga actgattgcc
      661 gccgcggcgg gagacgaaat tgatctgggt ggttgcctgc ccgaaaccgg aattcaaacc
      721 gagctggtcg gtttgcacgc tcatttgttc gatgtcttcg aggcgaggcg aaaccgccgg
      781 tgccaggttg gcgtcgaact tcatgcgggt cgcgctgccg accatgccgt ccatgttgct
      841 gccttcgtcc tcgatgggca gaccgttgaa cgttccctgg ccaccagcgc cggtaaaccc
      901 gggcacaatg gatgccagac tggtgagatc gcggccacta agaggcagat tttggatctg
      961 cttcatatcc accacagaac cgatagcgtt cgaggttgtc tgaagcaagg gagaggtctg
     1021 gccggcaacc tgcacggtct gcgtggtcgc acctactgtg agttgagccg taaggtctgt
     1081 gacctgggag gcctggacaa tgacctcgga atagaccttg ttggcatacc cggagcggct
     1141 gatggtgagg gtgtaggtgc cgatagggag attcacaaag gtgtaagcgc cactgctgtt
     1201 ggtgctggcg gtataggtgt cgttggtctg atgctcgacc agcctgagag tggtacccgg
     1261 tacaacggca cccgatgtgt cttgtaccgt aacgaccact ttcccctggg tgccggactg
     1321 cgcacgagcc gggagacagg gaagcgcgag gacgaccggc agaattgcca gtgaaagtag
     1381 aatccgccgc aggattctgc tgctcatgga tggacctcct gggaacgcgt aaaacgtttt
     1441 ctggcccagg gaattctatg gttcggttct ggggacacga ttccctggag ggtattgggg
     1501 accacatggg gagcaggagg gactcggtga cctgggagag tcctgcacgc cattgagtct
     1561 catcttgtat ttcaaattgt atgcattgtc aaggacgatg tgacgcagag cggtcgtagc
     1621 agggtggaac cgaaccgtat ggctcagtcg aacccggcca tcaatgcctg cagaatgagt
     1681 gacccgtcac ggctcctgcg tcgcattggc gcctccagac ttttcggcca gcagtcgtcc
     1741 catcctgagc cgcatctgcc tcgcctgcac atgattcccc agccggtcgt aagcgctcgc
     1801 caggctggca taaacagaag agttatcggg ttcgagtcgt cgtccaattt ccagatgatc
     1861 aatggcgttt tgcggctgat tctccatcag gtaaaccttg ccgagagcaa tttgtgtggc
     1921 tgagtagctc gcgttttcct gagctgactg ctcgagcgct tttcgagcct cctcaaacct
     1981 gggctcgcct ggcgcagctc cttcgtggag caggacggtg ccgaggagcg aaagcatgcg
     2041 atagtcgcgt tgacccgatt gaacggcctc gtggagaagc ttgtcggctg caaggagatt
     2101 gtcctcgtag aggttcttct gtacgcgggc cagatacccg atataggttt cgggggcaag
     2161 ttctgcggcg cgatcaaact caagtttgcc ctcctctccg aaccggccaa gctggctcag
     2221 gaagagggcc ttttcataat gcaggcgcgc cgagttgggc aattgctgga gacccagagc
     2281 gacgatctgc aggccgagag cgtagttctt gtgatcggta caggcgtcag cgatgtagtc
     2341 gtagagcttc tcgtctttgg gtgtagcggc tgcagcgcgg cgaaaactgc gaagcgcctc
     2401 ctgcggctgc gcattcccga tgtaggcctg cgcgagaagg ttctcgactg ccgcgctgcc
     2461 acgcccggag gcgtcgagtg catgaagcac gtcgatggca tccttgaatc tcccggttcc
     2521 gagatagcag attccgagat tgacgccagc cgcatagggc gcgatgccgg ctgcccgggc
     2581 ggcgagtaag tgcgggattg cctgctggag aaacccgttt gcggtgagtt gcgcaccggt
     2641 ttggagttct gcgcgagcgg tcgcctcgtc gtggagagct tgcggttcgg acctggaaac
     2701 cggaggagcc tgcagctttc cggcagattg cgcggcgctg agaaaggacg aagcgccaat
     2761 gagcagtgca attagcttca gcgcggcccc gatcgcgcgg acatgcatgg gaggtattgt
     2821 agggtggatt tcgggccaaa gcagcatacg aagttgtata cattcgttat ttgctgtata
     2881 aatcagcgcg tagccacaat ccccgaccca tcttcgtgac cgaagcgcgg caactcaggg
     2941 aaatcgcctg ttggtgcgcg gcggcccagg gctgaaatga atagacgacg ttttctgcaa
     3001 tctgtggctg caacaacagc cgccttccat gaattctttt ccgaggcaga aacatcggct
     3061 gcgcccatcg atcagtcggg gctggtccat gacatgctcc gcgacagcga cgcatcggtc
     3121 gaggggcaca cgcgtctttc cagcttcact cgtggcaaag agcaatggac tgtctacgag
     3181 gatcttcgga ctcgcgatgg tgcgatcacc ttcgtctcat cggaaggctc tgccagggtg
     3241 ctgacgaaaa gcgcagaagc cgtgtttgcg gaggatgaag cacagtacct cgggcttaac
     3301 ctcagcgaga tcggaatgtc cggcccggat ctgctggccg ataagctcct ggcgaaaggc
     3361 gaacctgatg aggacgcagt tcgacgcgcg gctccgccta tggattcggc cgctccggaa
     3421 gggcgctatt cgcggccgca ctggaatgcg atcgtgggca caagggagtg cagcgatacc
     3481 atgcccgttt atccggcagg cagcactcgg acatggcatc cggttcagta tttccccgag
     3541 ataaccaacg agcgcgcccg caaaagacag gagggtctgg ttgggggctg gatgccggcc
     3601 gtgcgcaagc tttttcccgg agacgatggc tctgtctttg aagcgatcgt cttcggcgat
     3661 gtgctggctc atgatcggtt tatcgtccag acctggcatc gcgtggcgca gatccgtgac
     3721 gggaaggtcg tcaaagtcgt ctacggctac agttatccgg cctacacacg gcgccggcag
     3781 aatcccgacg cgacggagtt ctatcttggc cttctggagt ttgccgaata ctgggatcgg
     3841 cagatgcacg atgtggcagc agtcagtctc ccggatgcca gctgggccaa catggcgaag
     3901 catgccttcg tgaaggagca gatcgttcgt cccggtggcg tctatcccaa atacggcgca
     3961 gtggaccgcg actactacgg gccggaatac gacggctttc aggacatctt caccatgtcg
     4021 ctttacgcga atctggagtg cggccgattc gcgcaggcac gcgatgtttt ggacgactac
     4081 ttcagccgtt tcgtgaacgc gaaaggcatg atcaacatgc gcggtcccga aaccgcgcaa
     4141 tttgggttga ccctgtcgct gatcgcccgc tatttccgct atacgggcga tacctctctc
     4201 ctgcgcaagc atcgggagaa aatcgaggcc accgcttcgt tgctgacgga gctgcacgat
     4261 gaggcgatgc aacttccctc aggcgacagg ggattcgggc ttattcacgg ctggtcagaa
     4321 tcggactcct gcctgatgga ggatccatcg atctggtgga agccgtattt ctccaacagc
     4381 gcctttaccg tgcggggatg gcgggacctt gcagaaatgt ggaaagacgt ggctggcccg
     4441 ggcggggaag agaattccag cgattggatg cgccgcagcc gcgagttgca cgtcacgctg
     4501 gtgaagagta tcgacgcgaa catcctgcgc gaccgcaagc caccgtatct tgggccactg
     4561 ccgggcacta aactgacctt ttgggaatcg atgcaacagg aacatccgag cgagcagggc
     4621 tggtcgcatc gcgcctacgc cgaactcctt caggcaggcg tgcttccgga cgtgcaaacg
     4681 gcagtagtta tcgactgcat gcgcgcctat ggcgctacca ctctcggcgt ggttgccaat
     4741 gtcgaacggc cccatcctca gggccgcgac attctcggat tcatctccta tggctacgcc
     4801 caggctctgc tcaccctcga ccgcatcgac gagtacctgt tgttcctgta ttcgcaccgt
     4861 tatcacgatc actcgcgcgg aagctggatg gctggcgaag tatccggcat caccggcgat
     4921 ggggcgctgt tctgcatccc cgcccagcag accatcccgc tgctgctccg ctggatgctc
     4981 gtcttcgaag accccgatgg tcagcgcctg gatttcggac gagccctgcc ccggcactgg
     5041 atcggaacga aaaaacccat cgccatcgag caggcgccta cccgctgggg ccgcgtagac
     5101 tatcggctgg aatcccacgg agatgcccag ctcatcgcca ctatcgggct gccgaagtcg
     5161 ggacccctgc cgccgacgat tcaggttacg tttcgcgttc ctcagggtcg atcccttcag
     5221 gggctcagcg tcaacggtca aagccagccg ctgggcggaa aaaacatgga tgcggccgtg
     5281 tttgccccag gcggcaagcg tgacttctcg gtgattgcga cattgacttg aacaacaatc
     5341 gcatctcctt cgccgatcgt tattcgtaaa caccgcgccg aaaatttgga cagcaaaaca
     5401 cgggaggaag catgaatcgc aggttgagtc gtagaacttt cgtgagtagc gcggcgtacg
     5461 cgggagcggc cgcccttctt ccccatgggg ccatgggcct ggcatcgcag cacacccaaa
     5521 aggtagccgc tgccaccggc gaagataccg ccgttcaata cctgaccaac gcggccgtcg
     5581 ctaaagtgca gtggaaagcg aagccctttc cgatgcccga ggtgcgtctg ctgcctggtt
     5641 tctggaaaga caccatggag ctgaaccgca gcttcctgta ttcgctgcca aacgagcgcc
     5701 tggcctataa ctttcgcgta acagcaggaa tcccgacgga cgccgatccg ctgggcggct
     5761 gggaagctcc tgactgcgaa ctgcgcggcc actacgttgg ccattacctc tcctcgtgtg
     5821 ctctgctgca tgccagcacc ggagatgagt tcatcgctgc caagggcaac gagttggtca
     5881 gcattctggc ggagtgccag gcgaaggatg gatacctcgg cgcctatcca gctaccctgt
     5941 atgaaaagct gcgcaagcat gagcgggttt gggcgccttt ctacacctac cacaagatca
     6001 tggctggcct gatcgacatg tatcagcaca ccggcaatca gcaggcgctg gagatggcga
     6061 cccgcatggc cgactgggct cacacctatg ccatgagttt taccgacgat gactggcagc
     6121 gcgtgctcct cgttgaacaa ggagggatga acgaggcatc gttcaacctc tatgccatta
     6181 caagcaacac gaagtatcgc gatctcggct tccgctttga gcaccacaag attttcgatc
     6241 cgctcgccgc gaatcaggat gcgcttgccg gcaaccatac caacaccaat atccccaagg
     6301 tcattggggc ggcccgcggt tatgagctca caggagacgg ccggtaccgg cagatcagcg
     6361 agaattttta tcgcatggtg acggagcatc acgcctattg caccggtggc accagcaatg
     6421 gtgaattctg gcaagccccc gatgccatcg ccacgcaact gggtcctgca gcggaagagt
     6481 gctgctgctc ctacaacatg atgaagctcg ctcggcacct gttcggccag aaccccgacc
     6541 cgaagttttt cgactattac gagcgactgc tgctgaatgt gcgctacgga acgcaggacc
     6601 gcaacggcat gctgatgtac tacgtccccc tgcagcctgg gatgtacaag accttcggca
     6661 ccgaattcga ttcgttctgg tgctgcaccg gaaccggatc ggaagaatat gccaagctca
     6721 ccaactccat ctacttccac gatgacaacg cggtctacgt caacctgttc atgccctccg
     6781 cgctggactg gaaagaacgc ggcctgaagc tggagcaaac cacaaagttc cccgacgatg
     6841 agcgtgtgac gttgaccgtg aagagcgcac cgtcggccac gaccgcgctg aagattcgtg
     6901 taccgtattg ggcgacccaa ggcgtcgcgt acagcattaa cggccagcca cagaacgtga
     6961 ccgcaacgcc gtccacctgg gtcacgctgg agcacgattg gaaagccggt gacgtcatca
     7021 caatcgacat gccgctcacg ctgcaccttg cgccgacacc tgacgacaag caggtgcagg
     7081 cggcaatgta cggcccgctc gtgctggcag ccgttctcgg caccgagggg ctcactactg
     7141 gcatgatcta cggcggttcc gggcctggag gaaacgacga cggctatcct atgcccactg
     7201 tggacatgcg cccgcacagg catcgcggcc ccgacggcaa gccggtggaa gggccgcctc
     7261 ccgagcccgg cgcaatctgg tttgagcgtg cggaaggctc gcgccgatat ccgctgcagt
     7321 ttcggacgaa ggggcgggga cccacgcaca cgctggtacc gctcaatcag atcatggacg
     7381 agcgctattc ggtgtatctg cggaacctca ccgcgtgacg cctcgcgcgc cgtgccgatt
     7441 ggaagcacgc ggccgtggtg tcgaatatcc tgttccctcg ggatgaagtt ctgtgcaccc
     7501 tattgcatga tgaggaagtg gtgattcgta ttgcggcagt gctgacggtc gtggtcagcc
     7561 tcgttgtggc tgctctggcc aatggcctgg ggcagtccgc atccggaaat gaccggctgc
     7621 ctgccgattt cgaagatgtg accgcgccgc tcggcgtcca cttcatccat caggcgccgc
     7681 acagttcacg aaaatatctc ctggagacga tgggttcggg agtcgcgttg ttcgactacg
     7741 acaacgatgg caggcttgat ctctatctcg tcaatggagc gcccttcgat gatttcgtgc
     7801 cgaagggcac catccccgaa aagaccgacc cgaaatactg gaatcgcctc taccatcagc
     7861 gagccgacgg cacctttgag gacgtgacgg agaaggcggg cgtcgccggc gtagggtacg
     7921 gcatgggtgt tgcggcgggc gattacaaca atgacggaaa cgaagacctc ttcgttactt
     7981 ccctgggcga gaatcatttg tatcgaaaca acggcaacgg aaccttcacc gatgtgaccg
     8041 ccgtggcggg agtgggcggc agcggttggt cgacctccgc ggcatgggtg gatctggacg
     8101 gcgacggcta tcttgacctg attgttctgc gttacatccg gtgggattgg gatgatgtgt
     8161 ggtgtggccc caaagacaac cgaggctatt gccatccgga tgccttcccg ccgatctcga
     8221 tgctggtgta tcacaacaac ggcaacggga ctttcactga gcaagcacac aaatcaggtc
     8281 tggatgtgcc gggaaaggcg ttggggattt ccattgccga ctacgatcgc gacggacatc
     8341 cggacatctt tgttgccaac gattcaatgc aggagtttct cttccacaac actggagccg
     8401 gaaaattcga ccaggtgggg ctcgagtccg gcatcgcagt ggatggggat gggcgaacct
     8461 ttgccggtat gggcaccgac tttgcggatt acgacaacga cgggtggccc gacatcttcg
     8521 tggatgacct ggcgaatcag aaatacgcac tgttccataa cgaccgcgac agtaccttta
     8581 gctacgatag ctttgttacg ggcattggtg gcctgacgct cctgcattct ggctggggcg
     8641 cgcgtttcat ggattacgac aacgacgggt ggaaggatct gttgatcgcg caggcacacg
     8701 atctggatac catcgagaaa gactttcctc agctgcactt ccgcgagcca ccgctgttgc
     8761 tgcgaaacgt ggacgggagg aagtttgtcg atgtcggccc tgtgtcgaac atagttttca
     8821 gggagagatg ggtgggtcgc gggcttgcaa ccggcgacat cgacaatgac ggacgtatag
     8881 acgccgtcat cacggaaaat ggcggtccgg tgcacattct gatgaatcga acgaagagta
     8941 cgaatcactg gattggattc aaactggtgg ggcatcggtc caaccgggac ggtatcggtg
     9001 ctgtgatccg catcgatacc gcaacaggat cccaatgggt gacagtcaca acgtcgtcga
     9061 gctacatgtc ggcgagcgat gagcgcgcac attttggact cggtcgggaa agcacggtgc
     9121 agaaggtcga gatccgatgg ccaagcggca tccggcagat tttgaccacc gtcgctggtg
     9181 accgttacat ccgcgtggag gaaccggctg ccagtgcggg caaataggcg agcgccctca
     9241 tggctcaatg tggtgccggg cgggagggcg agccgcataa ttcattgata acaagtaagt
     9301 tgctaatttc tgaatcggca accgaaagac acgtgggtgc cggcaagtcg gcgaaccacg
     9361 ggcgaccgtc cgtcgggagc cctgaaccaa ctcgttgaac cacatgagag ctggcacggg
     9421 gagccctccc gcacaatcaa caccatccca gaggggcgct cccctcatcc agaaaagccc
     9481 accccagccc gcggccttcg gctgcaacca aaaccaagtc gagaatctcg tagcgatata
     9541 ctgcagtgca gcatataatc gtgctgcact gcatcctacc tctctgcagg tgaacggaaa
     9601 tggaaagcac tgttattgcg gaacgaactg cgcctgcggc cgaagcgccg ttcagtctgg
     9661 cgggccgcac cgccctggtc actggcgccg gccgcggcat cggtcgcgcc atcgcgaagc
     9721 gcctcgcctc cgctggtgcc cgcgtcatgg tcaacgatct cgatgaggtc atgctccgcg
     9781 agtcagaagc cgaactggcc tgtcccgagc gtgtccgcca tgtgcagggc gacctcaccg
     9841 accccgccac ccccgccgcc atcgtccagg cgaccctgga tgccttcggc acgattgaca
     9901 tcgtcgtgaa taatgcgggt tacagttggg ataacgtcat ccagaagacc accgacgagc
     9961 agttcctcgc gatgctcgat atccatctgg tcacgccctt ccggatcctg cgcgccgctt
    10021 ccacgttcat tcgcgaagct gccaagaaag agatcgctgc cgggaaaatg gtgatgcgca
    10081 aagtggtcaa catcacctcg atcgctggaa ccgacggaaa ttccggccag gctggctact
    10141 catcgggaaa ggctggcgtc atcggcctca ccaaaaccct tgccaaggaa tggggccgct
    10201 acaacgtcaa cgtcaatgcc gtcggattcg gcctgattca gacgcgcctg gttcaggatc
    10261 tgaatgcgcc cggcgcaagc atggagatgc acggtcacca gatccgcctg ggtgttcaac
    10321 ccacgctcct ggaatccgtg aagagcgcgt gtccactggg gcgtttgggc acgccagaag
    10381 aagcggcagg aacggtcctg ttcttttgct ctccgctttc cgactacgtt accggcgaag
    10441 tgctgatctg tggtggtggg ctgcactttt agcaggatgc ttcttcataa ccgttgcggc
    10501 gacatgcggt tcgggatctg cgtccttcgc gaacagatag tcttcctcga ccagccagta
    10561 cacctcgagc cagtggtcga ggctgtgctc gtgactgcag tcgtgagaga gaggatgagg
    10621 tttttcctgc gattgcggag cgtgttcgag gagaattggc atagtcgcct ccggggtggt
    10681 cgcctgccag gggccgtcca ggtgatcctc agaacagcac agaaggaccg gagattgctg
    10741 tgacggcagt cacaccgggt ggttggccgc cacgtttcca ggagatggaa aactgccgac
    10801 gagatccgtc gaactccact tcagtccagc tccgagtagg cagcggtcgt caggaactcc
    10861 tgaaactctc ccgtgctgag ttctttcagg atttccgatg ccgaagtgaa tcggctggct
    10921 gcccaggctt tcgctcccat catcgatcgc atctggtcga gcacctcttt caacaccgat
    10981 tcgaccagat caaaagtgac gatacggccg tcctggaggt gagcgccatg gtgaatccac
    11041 tgccacagct gggcacgaga aatctctgca gtcgcagcat cttccatcag gttatagatg
    11101 ggcacgcagc cgttgccgcg cagccaggac tcgatatatt gcaatgcaac atcaatattg
    11161 cgctgcaacc cagcttccgt aatcgggcct tctgggacct ggagcaggtc cgtactggca
    11221 atccgcgact gcttctccgc aggaatccga atctggttct tctcgggcat gtaccggtcg
    11281 aaaacctcac gggcaatcgc taccagcccc gggtgggcga cccaggtccc atcatgtccg
    11341 gcgcgcactt cacgcagttt gtcctggcgt acccgctcca tggcttcttc attggctttt
    11401 tcatcgttgc ggatcggaat ctgcgccgcc atcccgccca tcgcgtgcgc cccgcgacga
    11461 tggcacgtgt ggatcagcag ttccacatac gaccgcagga acggctgctc catcgtgacc
    11521 gtgccgcggt ccggtaatac aaattccgca cggcctcgga acctcttgat gaagctgaag
    11581 atgtagtccc agcggccgca attgagtccc gccgagtgtt gtcgcaactc gtacaggatc
    11641 tcgtccattt gaaatgccgc ggctatcgtc tcgatcagga ccgtggcgcg aagggtcccc
    11701 ttcggaatcc cgacgtattc ctgcgcgaac aggaagacgt cgttccataa gcgagcctca
    11761 agacggctct ccagcttggg caagtagaaa tacggtgccg aaccccgctt caacaacgtc
    11821 ctgtagttgt gaaagaaata caacccaaag tcaaagagcg aggccgaaac tggcgcgccg
    11881 gaaaagcaaa ggtgcttttc caccatgtgc catccgcgcg gccgcataaa cagcaccgcg
    11941 ggattccgtc ccagctcgta gatgcgcccc tcgcttgagc accagctgat cgttcgcagg
    12001 tttgcatcgc gcagattgcg ctggccatcg atacagttct tccacgtcgg tgcattggag
    12061 tcttcgaagt cggccatgaa cacattggcg ccggaattca gcgcattgat caccatcttc
    12121 cgatccggag gtccggtgat ctcaacccgg cgatcgagca gctcgatggg aatcggcgca
    12181 cagacccagt ctccgtgacg gatgtcgtcg gtctccgcca gaaactttgg cagacatccg
    12241 cgatcaaact cctcctgacg ccggcggcgc gcctccaata gctgaaccct ccgcgcctcg
    12301 aagcgaaaat cgagttcgcg caggaactgc agcgcttcgg gggtcaagat ctcctgatcg
    12361 cgctcttcag acgaccagcc gtcggtgttg ctttccagcg ccgtcgtcac gatcttttcc
    12421 agggagctat ccatggctct cctcctccgc aaagtttgtt cgagccctga cctagatgat
    12481 ggcccgcaac tctgacaagg tcggcgcatg atcggcagga ctgtcgaact gctcctcttc
    12541 cgtcgatcct ggcagcgcgc aggtggaaat ttgtcccgag gcgatggtgc tcgcaacccg
    12601 gtcgaaatat ccggttccaa cgaatcgctg atgcttcacg gcgctgtagc cgtgcttctt
    12661 cgcctgatcg aattcccgtt cctgcagccg agcgtaggcc gtcatgcccc gatgcgcgta
    12721 gtcgtgagcg agttcgaaga gcgaaagatt gagcgcgtga aagcccgcca gggtcacaaa
    12781 ctggaaccga taaccaatcc tggcaagctc ctgctggaag tgcgcgatct ccgcctgact
    12841 cagcttgccc tcccagtgga acgagggaga gcagttatac gccagaagct ttcccgggaa
    12901 ctgggagtga attccggctg caaagcgcgc ggcttcctcc agattcggtt ccgacgtttc
    12961 gcaccacacc atgtccgccc agggagcata cgccagtccg cgcgcaatgg cggcttccac
    13021 tccgccacgg aagcggaaga atccctcgac cgtccgctcc cccgtgagga attcgcgatc
    13081 ggttgcatcg cagtcgctcg tgatgagctg cgccgcgtct gcatccgtcc gtgcgatcag
    13141 aatggtcggc acatcgagca catccgccgc cagccgcgcc gcaatcagct tctgcaccgc
    13201 ctcctgggtg ggtaccagaa ccttgcctcc catgtgaccg catttcttcg ccgaagagag
    13261 ctggtcctcg aagtgcaccg cggctgctcc ggcttcgatc atcgccttcg tcagctcgaa
    13321 ggcgttcaga acgccgccga accccgcctc ggcatcggcc acgatcggca gcatccagtc
    13381 gatgttttcg tgcccttcgg catgcgcaac ctggtccgcg cgcatcagcg cgttgttgat
    13441 gccccgtacc agcgccggca cgctgttcac cggataaagg ctctggtccg gatacatctg
    13501 tccgctcagg ttcgcatcgg cggccacctg ccacccactc agataaatcg ctttcaggcc
    13561 ggcctgggcc atctggacgg cctggtttcc ggtcagtgcg ccgagggctg caacatattt
    13621 ctgctcgtgc agtagccgcc acagcttctg tgcaccgctg cgcgccagcg agtgctccac
    13681 atagaccgtg ccgcgaagcc tcttcacttc atcggcgctg tacgcccggc tgatacccgt
    13741 ccatcgtggg gaatggaaac cattattgcc tgagctcttc aacatctctt gctctcctcg
    13801 ggcctccatc gtcacgcgga ggcgctgtca ttgagaagcg agtaaaccct ggccggtgat
    13861 cgtcacgcaa gagggactcg tccgattcgg acgagtggac gaaaccgctc cgggagcatc
    13921 ataatcggcc acagatgtct gatcattctc agaaatcgga gtcttcgcga ggaggcgaaa
    13981 tgcagacaaa ctcaccttcg gcgcggttgg actcgcaccc gccggatcga tcgtccgatt
    14041 cggatcatac cccgaacaat acccggatcg gccacctggc cgagcgcctc agtcacagcc
    14101 tgcatttccg tcgaatcgcc gccggcaagg ccatccttga cgaatccgag ccgattctgg
    14161 cgcggctcga tcccggcgcg ccgcaggccc tccgcgtgtt gttgctggtt gcacagtggg
    14221 ttgacgtcgg ctatcgcgac caccgcttcc tcgaaaccct gctgagaccc ttctccgccg
    14281 acctccgccg ccgccttcct gtcgttgact atttacgcct gcggatggtc gatgcctttc
    14341 gcgccctcgc catggaggat gttgacgccg ccattgccgg cctcgacctt gttctccgcg
    14401 ccgagagtga actctgcgat gagtcactgg ctgcgctcgc gcatttctgg aaaggacgcg
    14461 ctcatcggcg aaaaggcgaa tatgagactt ctcttctgca catcgctcgc gcccgcgaac
    14521 tagctgagaa atcgcatgac gatgttttca ccgcggtgat ccagatccat gagagctggc
    14581 tgctctttca gaagggccag cgccgcgagg cgctgcgcct gctcacgcac gccgagcacg
    14641 ttctccagcc cacagaccac tacatcgccc tcggcaatat cgagtcggcg cgcggccgta
    14701 tcgtgcgccg ttccggcgaa tacacgcgat ctctcgagca tttcgaacga gcccgggagc
    14761 tttactccca gggggatccg aaccacctca acatggcccg ggtgctggtg aatggcgcct
    14821 acgttcgccg cttgctcgca ttgcagttac gcaggaagat cgaccggcga acctggcgcg
    14881 acagatcctc gtccggcgac ggtagcctgc gggcacgcta ccagcagctt tcagagtctg
    14941 ccatccacga cctgaaacag gcgcgccaga tctatgccac gcacacgcac accggcggca
    15001 tcggcaatgt gcttttcaat cttggttatc tctacctcga tcgcggcgac attgaccacg
    15061 cagcgcatca ggcaagggag gcctaccacc tcggcctgga gaaaagtgat catatcctca
    15121 tggcgcgcgc ccgcatcctg caggcggccg ccgaaaatgc ccgcgttgaa gagcaactgg
    15181 gtgaggatgt cgatatcgcg gtccacgcca atcgcgcgag ggaagagagc gacgaagcgc
    15241 tggccctcgc gcgcggcaca caaaatcggc gcctcactgc cggagcctgg attgcacgag
    15301 ggatcaccgc tgcaaatgac ttctttcagg actgggaact ggcccgccat tgcgccacgg
    15361 aagcagccgc cctgatggcc ccggacgaga acgatcacct taccgaagac attgctgctc
    15421 taaagtcaaa gatccttcag gtttccggca tcagcgatac cctgcgcagc tggtccgaag
    15481 gaatggtggg cgacaagacc ttccagcaga tcacggaaga attcgcggaa atcgtgatcc
    15541 ccaaagtgtg gctgcgcgag aaccgaaaga tctcccgcgt cgccgaacgg ctttccgtct
    15601 ctcccaagaa agtgcggcgt attttgcgca atgccggtct gcggtcacgc agctgaggga
    15661 ttttcagtac agagcctacg ggttttccat cacatcaaag aatcaactgc gccatcttgt
    15721 gacaggcctt tcagtcgcat caacaaagac ttaaattaag agcaattccg cgtccgccat
    15781 tcgctattct tcaacgccct cttccttcct gctgaacgcc acggccccgc ggaaagggcc
    15841 agcttgcatc ggcggcagag cgcccccttc cactccgcct ttcgatgggc aattcctccg
    15901 gtggtgcgac gccatgggcg ctcgtgttca cttctcgcgc cggaattggg gagttccaca
    15961 ctctcctgtc cagcaccagg tcagaatgca ccgcctgcag ccggacctca tctggctccg
    16021 ggctcctgcc ggattccatt caccatcctc ccgattgcat ggagccacgg cccggcgggc
    16081 ctctcacctg ggtcccgctg tgcttcgggc gcgacgatcg ggaccagcct gaagggagcc
    16141 ggcccatgac ccatcgtgaa gttgtcgtgc tgagtgccgt gcgcacagcc atcggcaaat
    16201 tcggcggagc gctgaaagac attcctccca ccgaactcgc cgcgaaagta gtgtgccaga
    16261 gcgtgcagcg ctctggtctc gctccgagcg agatcggcca cgtcattttg ggcaacgtca
    16321 tccataccga tcaaaaagac atgtatcttg cccgcgtcgc cgccctgcgc ggtggcattc
    16381 ctgtcgaaac cgcagcgatg accgtcaatc gcctttgtgg cagcggtctc caggccattc
    16441 tcaatgcggc gcaggccatc gcccatggag acaccgacgc cgccattggc ggcggtgtcg
    16501 aatccatgag ccgcgctcct tactggttgc cctctatgcg ttggggcgcg cggctcaacg
    16561 acgcaaccgc tgttgatgct cttgttgcct ccgtcaccga tcccttcgac aacgtccaca
    16621 tggggattac cgcggaaaat gtagcacgca aatggggaat ctcccgcgaa gaccaggatg
    16681 cactcgctgt ggaaagccat cggcgagcga atgaagccct caaatcgggc aaattcgcca
    16741 gccagatcgt tcccattgaa ctcaaaacca aaaacggtcc ggtcttcttc aataccgacg
    16801 aacaacctcg ctccgacgcc acactcgaga acctcgccaa acttaagccg gccttcgatc
    16861 ctgcaggcac ggtaacggcg ggcaatgcct cgagcatcaa tgatggcgcc gctgccgtgg
    16921 tcctcatgga acgttccgcc gccgaagagc gcggcctgaa acccctggct gccctggttg
    16981 actatgccgt cgtcggcgtg gagcctgccg ttatgggcat cggccccgtc gccgccgttc
    17041 gcaaactgtt tgagcgcact ggcctcagca aggagcaaat ggaggtcatc gagctcaacg
    17101 aggcattcgc agctcagctc ctcgccgtcc ggcgcgaact cgacttcccg cccgaacgaa
    17161 ccaatcccaa cggcagcggc atctcccttg gccatcctct gggagccacc ggagccgtcg
    17221 ttactgtaaa agcgctgcac gaactgcgcc gcgtgaacgg ccgatatgcg ctcgtcacca
    17281 tgtgcatcgg cggcggccag ggaatcgccg ccatcttcga aagcatgaac tagcggagaa
    17341 aggagtctta ctatggacac cgcagttctc atcgaaccac agccatcctt tgcccggcct
    17401 ctcgagggca gagttgccct ggtcaccggc gcctcccgcg gaatcggccg cgccatcgcg
    17461 cgcgaactcg ccagtcgcgg cgccatcgtc gtgatcaact tcttcaacag catcgagcgc
    17521 gcccagcagc tgcgcgacga catcgaatcc gccggcggag aaaccagcct cctgcaggga
    17581 gatgtctcca accgccagga agctcggcgc atcatccacc atctgctcga ggtctacggc
    17641 cgcatcgacg tcctcgttaa caatgcgggc attacccgcg atcgttccat ccgcaagatg
    17701 actgacaacg agtggctcga agtcatcgag accaacctca acagcatctt ctactgcacc
    17761 agcgccgccg tgccctccat gatcgagcac aagttcggcc gcatcgtgaa tatcgcttca
    17821 ttctccggtc aggccggcaa tttcggtcag gcaaattatg cggcgagcaa aggcggcatc
    17881 atcgctttta ccaaggtgct ggccctggaa ctggcgaggt tcaacattac cgcgaacgtt
    17941 gttgcaccgg gcttcacctc aacggacatg ctcgccgccg tgcccggcga cattctcgac
    18001 cagatcaaga cgcgcattcc tcttggccgc ttcgcgcaac ctgaggaagt cgcgaaggcc
    18061 gccgccttcc tgatctgtga tggagactat gtcaccggac agcaaatcaa catcaatggc
    18121 ggaatctata tgtagtgcga ccattgatct ctacccggag cgggcgctca gccacttctc
    18181 gatgtgcggc cacgtggtcc tgggcgctcc gcttccggcc atcattccga tgtgcccacc
    18241 cggaatccgg tagagctcct tgtcgcggct ccccgctttg gccagcaggg tctccgactg
    18301 gcaggcgggc gtgatgtgat ccccctccgc aatcacagtc aatagattcg cccgcattct
    18361 gccaagatcg acgcgctctc cgcgcaccac gagctcgttc cggatcagac gattctgccg
    18421 ataaagctcg acaatcagtt gtcgataggc ggcgccggcc atcggaatgt tatctgtcac
    18481 ccacgtgttc atcgcgtgcc aggactcgac cacgcgcgga tcgtccaggt tatcccacag
    18541 cttgcagtag ttggtgatgt agttctcgat cggcttcagc gcttttgccc ccgagtcaat
    18601 catctcggca ggcatgttgc cgaaagcatc gaggatcagt tcgaccttga agtaccgctc
    18661 atcggtccac cgcgcaaacg tgacgctgtt ccggttcgag aaatccagcg gagcggtcag
    18721 cagcagcaga ttgcgcagcc cttcgtcagg tcgcagcgcc gcgtagatgg tggtcaggat
    18781 cgctccgatg caccacccca gcatgctgaa ttcctcgaca cccgcaatcg ccttgacctt
    18841 tcgaatcgca cgcggcagat actcgagcgc atagtcatcc agtttgaggt gccgatcctc
    18901 atagccgggc gcgccccagt ccagcaggta gacatcgtat cctcttccca ccatgtactc
    18961 gacaaagctg tggccaggcc gcaaatccag gatcgacggc cggttcatga tggcgaagat
    19021 cagcagcagg ggaagacgat gttggtgtgt tgggtccacc gtgggtctgt accgatagag
    19081 cttcgcttta ttgagcgccc acaccagatc ctttggggtt tgcgcaatcg gcgccctggt
    19141 agtcagcaca cggttgaagg tccgcagccg cttttcgaac ccagccaccg cacccgacat
    19201 ggcgaaagcc ggttccatgg ctacctccgc gtacggctaa gcttgcgccg gacaaagggt
    19261 tgtcgtgccc ctggccgact gacggcagtt tcgtgatccc ctccctcgcc cgtcctcggc
    19321 gtaggagtgg ccgcagccgc tttgccgacg ccggattgtg gccccgatcc gttctgctcc
    19381 atgtgctttc gaatggcatc cagcttggca tccatgtcat cgaggcgcat ctcgagattg
    19441 gtgaagcgct ccgcgagtgc cgctacctcc tgccgcgaag gcaactccat ttgctgcagc
    19501 gtctgcagca tcgacttgtc aagcgcttcc cggaaaggag cgatcgccgt caggtagccc
    19561 tccagcatgg cgccggtcgc ctgcgcatac gcttcggtgt tgaccgtctc caccatggct
    19621 ttcgccatcg catccagata cgtgtcgcgc atggagcgga acggctcgaa cgggtcgtac
    19681 gtcttagagg tcttctcctg agtttcactc atcaggcgca cctccttctt cgacacatga
    19741 agcataaacc cgcccggacg ggcaggctat gcttcccgtc aatgctgtga aaccgctctc
    19801 aacgaactgc catgtccagc agttctttct gggcatccac gatggcgtcc acgccgcgct
    19861 gcatcgaacc ggctgccatc tcaaccggac tgccttctcc gaactgtttg ctcgaggtct
    19921 ccaccaccgc cttggcctgc gcagcggact ggtcgagtgc cttcttctgc atggcaacga
    19981 cgcgctcaat ggacttcttt gtgaactggt ccacctcttc attggcgttg gtggctgttg
    20041 ccgcccgctc cttcagcacg tcagcaaagg ctttgctctg ttccaccacc aggtcgatcg
    20101 ctgccttctg aacctcggcg tagtgatcga atccgcttgc cccgagttcc agcaggaaca
    20161 gtcccggcgt cccgggtacc ttctgtattg cttttttcca cacatcgatc aattcagcgt
    20221 tttgctgtat tgcaatatca aggcccttct tctgaacatc ggcgattctc ccgacccctg
    20281 cggtgaaaag ctttgccatg gactcggccc agtccacctt ttcctttccc ggaggcccca
    20341 tgggttccct gaccctctgc gctgtgctca tatgagtccg tcccttcttc tgctgcacga
    20401 tatgctgcag tgcagcatat cgtgatatta cgctgcccgg cccggctgtc aaggttaaaa
    20461 tggccagaga tgccgccgaa atccatcctg atcaaaaaat acgaaaatcg gcgcctctac
    20521 gacgtcacca acagccgcta cgtgaatctc gaggaggtcg cccaactcct ccgtgagggc
    20581 catgacgtcc gcgtcgtcga tgcagcttcc agtaaggaca ttacccggct catcctcacc
    20641 cagatcatca ccgaagacgc caaaaccacc gactccacct ttcccctcga cctgctccgg
    20701 cagatgatcc tcacttcggg ccgggccagc caggagaccg cacttcgtta tatgaaagcg
    20761 atgctcgacc tctatcagga cacctaccgg gccatgtcgc cggcggtagg ccccttcggc
    20821 atgtttcggc ctggcttcgg gggccccgtc gcgggcagag accagcccat ttccatgaac
    20881 gcgtccggtg aagagcccac gaacagagct ccgcaggaaa cgaaacaagt ggacgagttg
    20941 aagcagcgtg tggccgatct ggagcggctg gtatcgtccc tcaccccatc caaagtggct
    21001 cagcgcaagc cgcggcgcag caagccaggt gcaaaatcgg cacgcctgcg ctagggtgag
    21061 gtctccaccg gagtgatcgt ggtttcggtg cggccgtcag tgttctttgc cagtcccgaa
    21121 gaattcgcag atagcttccg cactcggctc cttcgctcag tcggacgtcg gcagaaccct
    21181 ggcagtctgc gcatcgatga tcctgattcc ttgctagcag ggcagtttct aggctgcctg
    21241 cccgattgcc acatggcctc cacggaagct ttaatgggct ttattgtggt gccgggaggg
    21301 ggggtcgaac ccccatgacc gcaagggtcg gcggattttg agtccgctgc gtctgccagt
    21361 tccgccatcc cggcgcgtgg gaaggggtag cggttagtat agcgcccggc cccggacgtg
    21421 cgagttcctg gtcgtctggt ccctattgtg tccgtagcgc gaacatgatt tatccaccag
    21481 cctgctttgc gaactgcgag gaagaaaaag cgatcgtacc gcacccgagg tcaacggtcc
    21541 tccgaatcga ttggccacga ctccgtgcga aatcgatatt aggcggtgcg aagcaggagt
    21601 tgggacgcga gagaagggcc atgctgcagt aggaatcgag aaagccgttg ttctccaagg
    21661 tgaacagcaa gaagagattg gaagatagcg gggtgggcgg cgaaagaacg gagcgttcga
    21721 gcgcggagac gtggacaggc gtccaggagg aggagtaggc gagccatacg tcgtggcacg
    21781 gcgagcagcg ccggatgacg ggattcgtaa agatcaagtg agcccgagac cagcgaatcg
    21841 gcgagaagga tggcttggcg gaaggccagg gcgagccctt ctccagtaat ggcatcgatg
    21901 gaaccggagg catcgccgat gagggctacc cgaccgcgag tgacccgcgg caggcggcgc
    21961 gtaagagtga cgcagccccg ttcagaggca aggggctcgg cgcggtgaag gatgcaactc
    22021 gatgagggaa gcgtattgag gatcttagcg aggcgcgcgt gcggatcacg cgtcatggcg
    22081 gagatgcaaa tttcgcctgg agctgtaggt gtgatgtacg cctggccatg aaagccccag
    22141 tggacttcta cgtgtctggg tgagggggtg ccgggagcga ggcgaaaatg gccgcgaaag
    22201 ccgaagcggc gtgttccggg gctggcgggg tcgagcccgg accagcagcg gacgcgggac
    22261 gaactcccat cggcaccgat gatccaacgg gcatgaaccc tgccggatcc ggacgaaccg
    22321 acgaggctgg gaggctcgcc ggagcggagg atgaccggcg tatggaaggc gaagcgcacg
    22381 ccgagggcgg cggctcggtc gcaaagaagt tggtggaggg tggtgcgacg cacgcccagg
    22441 ccagcgagat tggggaaatc ggccgagaca cgagagtggg catcgatgaa cgtgattccg
    22501 gcgaagggga ggccttggag atcggggaac acgccgagag cagcgagggc gcgccgagcg
    22561 tccggcataa gaccttcgcc gcacggttta tcgatgggag cctggcgggc gtcggcgacg
    22621 aggacatcgg ctccacggag acggagtgcg atagcggccg ctagcccggc tggcccggcg
    22681 ccgacgatga atacgtcaca catgctgtta cgtaacggcg tcaccattcg agcaggagca
    22741 tttcggcgtt gaagccgggg cccatcgcag cgaggatgct cctggtgccg gagggggggc
    22801 ggcgctgctt tatgacctca tcgaggacaa ccagcacgga cgccgacgag agattgccta
    22861 cacggcgaag agcctcccac gagacatcga gatcgtcgcg ggatagacca agggcctcct
    22921 gggtggcttc gagcaccttg gggccgccgg tatgcatgat ccaggatccg atatcgctgc
    22981 gggtcagttg gtgcacggcc agaaattcat caacatttcg tcccagattc tcgacgatga
    23041 ctttggggac atcgggagag aggacgatgt tgaagccctt ctcggaaacc gaccagccca
    23101 taacctcttc ggtattgggg tagaagacgc gatcggtcgc gacgatacgc gggccccggc
    23161 gctggacctg atcgccggca acgaggacgg cggcagcgcc gtctccaaag agtccgacgg
    23221 agatgaggtt cgcgatcgac ttgtcgtcgc gttgccaggt gagagaacag agctcgacag
    23281 aaagcagcac agcgacctga tcgggatagg cgcgcacata gtcagcggcg cgagagagac
    23341 ccgcagcgcc ggcgacgcag ccgagtccga agatgggatt gcggcgaatg tgcggtgaga
    23401 gcggcatgcg gttcatgaga cgcgcatcga tcgacgggct ggcaacgccg gtgactgaaa
    23461 cgaagaagat ggcggcaagc tgatcggggg aaaggccggc ctgcttgaga gcacagtcga
    23521 tggagcgctg cccgaggtcg agagcagtgt cgatccacgc gtcattggtc ttaccgaagg
    23581 tgtcgaggtc gaggtagtcg gtcaacgggc gcgaaaaata gcgcccatcg acgccagtgc
    23641 gaaggtgaag ccgctcgatg acagaggtga gttttgcgtc ggtgccccag tagggaagga
    23701 gcgcatcgag aaactcgcgt tgggtgatgt agtgctcggg gaacgccgta gcggtggcaa
    23761 gaatatgcat gcagttctct ggattgatga gacgttgtct ttctgcgatt tgtattggga
    23821 tgcggcgcgg aaaaatcggg cgttgccgcc attctccatg atgggaggcg cttttgggcg
    23881 cggtgtcaag acgaagtcag aagattgtcc gcaagaccat catcgccggt gacgcgtgga
    23941 gagtcggaaa gcggaaattc aaccaggatg cgggccccgc cggtggcgcg attgctggcg
    24001 gtggcgacac cggcatgcgc atgggcgacg gcgctgacaa aagcaaggcc aaggccgtgt
    24061 ccctgggaat tgctgccttt gacaaagcgt tgaaggacgc gcggaagcag gtccatgggg
    24121 aagccaggac cgtcgtcttc gacggcgact gaaccagtcg atgcgcgcga tgtgacaacc
    24181 accgatatgt gcgcgccagg accagcgtag cgaagctcgt tttccatgag attggagaga
    24241 aggcgcgtgc acagcgacag gtcggcgacg atggggacgg gttcaggagc agaaacagtg
    24301 aggtgctgtt gggcgtcagc aatggccggg gcatagagtt cggcgaccct gtgcacaaga
    24361 tcgctaaggt caagagactc gcggcgcagg cggagcgcgc caccgtcggc ttcggcaagg
    24421 tcgagcgatg tggtgacgac ttcggacagc cggtcgaggt tctcgatggc gcttgcaatg
    24481 aggtcctggg attgctcccg atcctccaga gacaaggcga tttcaaggct gccgcggacc
    24541 gaggtgatgg gacttttaag gtcatgcgcc acggaatcgg tcagggtacg gagttgattc
    24601 accgaagcgg agatccggtc gagcatgctg ttaaacgtgc gagccaggcg ggcaatttcg
    24661 tcatgctggg taccgatggg gacgcgagtg ccaaggtcgt cggtccggat gctggcggcc
    24721 gcgccggtaa tgtcatcgac tcggttgagc aagcggcgaa ggccaaagag cgtgaggaga
    24781 aagccaaagg cgaccataaa aagccacccc agaaagaacc ggaccagaag ctggcggagg
    24841 agatgttccg cgccgcggtc caggaggccg agataaatat gcccggctcc aggggcgacg
    24901 tcgaccgcga cgacacggaa tggctcagca aatcccgcga cgcgcagact gacggcctga
    24961 tgttgcggga tattcagcgt ccgcagaagt ccaaggaatg caccactgtt ttggggtccg
    25021 acccaaagcg gaggctcgga ggtattgccg atctgggcga agaagacggt gttgcccccg
    25081 gtgttcgccg tgccgccatc ccaggagaac tcctgggtgg ccagttcggc gacttcctca
    25141 acaatgcggc tgtagagcgc gtcgcgcggg gtgctgaggg cgacctggcg aagggtctcg
    25201 ctttctccgg tgagccacga atcgctgcgc tcgcggactg tacgggcaac gatgaagtac
    25261 atggcgagga agaccgaggc gctggcagcg gcaaagacca aagtggtttg cagcgcgaga
    25321 cgccaggcgg cggtgcgata gaagaaacgt gcgagtcggg agaacattcg ctattcgcgg
    25381 agtaaatatc ctacgccacg gatggtgcgg atcaggggac gctgcgattc gcggtcgacc
    25441 ttgttgcgaa ggcgatggat gtggacatcg acaatgttgg tggaggtgtc gatgcgcatc
    25501 ttccagacgt gatctagaac catggcgcga gtgaccacgc ggccggcatt gcggcacagg
    25561 tattcgagca gggcgaactc ctgctgggtg agctgaagct cgatgccggc gcgccgggct
    25621 tcgcggcgga tcaggtcgat ttcaaggtcc gcgacgcgga gacgggtcgt ttccgactga
    25681 ccgggcgtgc tgcgtcggag cagggagcgg aggcgagcct gcagctcggc gacggcaaaa
    25741 ggtttcgtca tgtagtcgtc gccgccgcgt tcgagcccga gcacccgctc atcaacggag
    25801 cggcgggcgg aaaggatgag gaccggcgac gagaccccct gggaacgaag gcgctggatc
    25861 agatcgaggc cgtctccgtc gggaaggtta aggtcaacga caagggcatc gtgaacgccc
    25921 tcgagcgcga gcctctcggc ttcgcatccc tgagcacagg catcgacgcc aaagccggat
    25981 tcagcgaggg cccgggtcag gaaatcgcga atggtattgt cgtcttcaac gaccagcaac
    26041 cgcatgaata ctccaggatg gccgcgcagg gcggccatga ggtcttcgca agagcgcgcg
    26101 gacgagttgc ggattgctct cagggtactt ctcagcgaag gcagttgaaa ttacaaacgc
    26161 ttcataatgc gtagcctttg cgatttcatg gaatggacgc gagcggaatc ccggtggaga
    26221 gccggatgcg agcgcccaca aaatgacatt ccggtgtcgt gcgcaggtga tcgcgaaagg
    26281 cgtccaaacg cgttttccga ggtgcacttg aatttgtttc ccacaattga cccgtgctgg
    26341 ggcgggttgt aacgttaaag gttcgtgctc gaccaatctg cagttctgtg aggccttgtg
    26401 tccaccaccg ctgctttgcg taagacccca ctcaatggcc tgcatcgccg tctgggtgcc
    26461 aaaatggtcg attttggcgg atgggatatg cctgtcgagt attcgggcct catcgccgag
    26521 cacatggccg ttcgcaccgg cgtcgggctg ttcgacgtaa gccacatggg cgatatccag
    26581 tttcgcgggc caggatcgct ggcggcggta cagcacatct cgatgaacga cgcgtcacgg
    26641 ctgcaggtgg ggcaggcgca gtactcggcg atgctgtatc cggagggcac gtttgttgac
    26701 gacgtgatcg ttcacaaatt cagcgagaac gattatttgt tcgtaatcaa tgcagggacg
    26761 cgggagaagg actaccagtg ggtgcggtcg caggcgcgcg gcttcgactg ccacgtgagc
    26821 gactacagcg acctttacac gcaactggca atccagggac cgaaggcgca ggaaacgctg
    26881 caaaagctga cccagacaga cctgagcaag gtgaagttct actggttcac gtggggaacc
    26941 gtttgcggcc tgccgaatac gctgattgcg cgcaccggct acacgggcga agacggattt
    27001 gagatttacg tgccgtcaga tgaggcgacg agcgagcgtg tgtggaacga ggtgatcgag
    27061 gcagggaagg aatttgggat tgtgccctgt ggccttggcg cgcggaacac gctgcgattg
    27121 gaagcggcgc tgtcgctgta tgggcatgag atttcagaat cgctgaatgt gtgggaagcg
    27181 gggctggatc gcttctgcaa gctggagaag ggggagttcg ttggatcgga ggccctgaag
    27241 aaggtgcagg cagcgggcgg tccagagaaa agactggttg ggttggaagt tgttgagcga
    27301 ggaattgcac gcgatgggta cagggtgatg gatcccgacg gcaaagaaat tggctacgtg
    27361 acgagtgggt cgccggcgcc cttcctgaag aaaaacatcg cgctggccta tgtgccacgg
    27421 agcctggcag aagtggggac ggaggttgcg gtagagatcc gcaataaccc ggtgaaggca
    27481 acagttgtgc ccacaccgtt ctacaggaag ccaaagaaga cgtaacggtt cgcgaagttg
    27541 ccacgggtcg cgaatcaagg ttgtgcagtt cgtgctgcag gttccagaga acggagcccg
    27601 tacaatgagg gagagagttt cagcgatggc ttatccagcg aattatcgat acacgcgcga
    27661 gcatgagtgg attgacgtca gcggaaagac cggtacggtc gggattaccg accatgctca
    27721 ggactcgctg ggcgatatag tttttgtcga gctgccgaag gtcggcgaca aggttgagaa
    27781 gggtaaagta ttcggcagcg tcgagagcgt aaaggcggtg tcggatttgt actcgccggc
    27841 gagcgggacc gtgacggcaa tcaatgaaga actaaccaca tcccctgaga agatcaacac
    27901 cgatgcccat gcagcctgga tcatgaagat cgagctgagc gatgccgggg atgtgggttc
    27961 actcctgtcg gcggaagact acgagaagta cgtgaaggaa gagaccggac actgagaact
    28021 cctcacacca gagatcctgg tttttatcgg gaagtgaaat cccgagtctg agagcccatg
    28081 cgctatttgc ctaaatcgga tgcggagcgc gcggcgatgt tgcgcgacat cggcgtgtcg
    28141 tcgattgacg atttattcgc ggttatccct caggagtacc ggctgcaacg ggatctggag
    28201 attccgcggc agatgggcga gtcggagatc gtagatcact tcaaggctgc ggcgaatagg
    28261 aacgccaacg gttatgcgag cttcctggga gcgggagtct atcggcacta caggccggtg
    28321 atcatcgaca caattgtcca gcggggagaa tttctcacgt cctatacgcc gtatcaggcg
    28381 gagattacgc aggggacatt gcaggcgatc ttcgaatttc aaacgatgat ctgcgagctg
    28441 acggggatgg acatcgcgaa tgcgtcaatg tatgacgggt cgacggcgtg ccccgaagcc
    28501 gcgatgatgg cgatgcgcgt gaccggccgc gatggcgtgg tggttgcacg aacggtgcac
    28561 ccggagtacc gcgaggtgat gcgcacctac gcacagcatc gcgagaagga aagcgggctg
    28621 aaggcgcagg aagttggata cgatccagag ggtacgagtc acgcgggccg catcgacctg
    28681 gctgcgctgg aagcggccgt gacggaggag acggcgtgcg tgctggtcca gtcgccgaat
    28741 ttctttggag tgattgagga tgtcggggcg attgcggaga ttgcgcacag gaagggtgcg
    28801 ctgttggtag tcgcgattgc cgaggccgtg tcgcttggca tcgtaagacc gccggttgag
    28861 gcagacatcg tgatcatgga ggcgcagtca tatggcgtgg ctccgagttt cggtggacca
    28921 ttctgtggtg tgattgcggc gaaggaaaaa tacgttcgcc agatgccggg gcggctggta
    28981 ggccagacaa cggacaagca gggccggcga ggatttgtgc tgacgctgtc aacgcgcgag
    29041 cagcacatcc ggagagaaaa ggcgacgtcg aatatctgca cgaatcaggc gctggtagcg
    29101 ctgatggcaa cggttttcat gacggtgtac gggaaagaag gcatgcggga gctggcgctg
    29161 cagaatctgg cgaaggcgga ttatgcggcg aagactctgg gtgcgcaggg gaaactgctg
    29221 tttgatcggt cgccgaggtt caacgaattt gtgctgcaga ccgatgaggc gccggaaacg
    29281 ctgaacgctc ggctgctgga acaaaagatc atcggcggcg tggcgttgaa gaattggtat
    29341 ccggagttac ggaatgcaac gctgtggtgc gccacggagc tgacagcgaa ggaacagatc
    29401 gatcgggcag cgggggtttt ggcggcggcg acagcagcag tgggggcacg atagatggcg
    29461 gcgcagatcg aagagaagtt cgttggcacg acgcgcaagg tcaccgggca cctgacgcag
    29521 aatgagggcc tgagcttcga gaagtcggtg ccgggcaaga aggcttaccg gctggcgcct
    29581 ttggatgtgc ctgaagttga tccctcggct ctcctgggag aagcccagcg ctcggagagc
    29641 gcgcagttgc cggagttgag cgagatcgaa atcatccggc atttcacacg cctttcgacg
    29701 tggaactacg ccatcgatct ggggatgtat ccgctgggca gttgcacgat gaagtacaac
    29761 ccgcgtgtga acgaattcgt gtcgcgcctg gaggggatcg cggaggcgca tccgtatcag
    29821 ccggaggagc tatcgcaggg atcgctgggg attcttcggc tgctgcagaa atgcctgatt
    29881 gagattacgg ggatggacgc gatcacattg cagccggcgg cgggggcgca cggcgagttc
    29941 accgggatcc tgctggcgcg ggcctatcac gagtcgaagg ggaatccgcg gaagagggtg
    30001 atcattccgg actcggcaca cgggacgaat ccagcgacgg cggcgatttg cggctatgag
    30061 gtcgttaatt tcaaatcgaa cccgaacggc gggatcgacg tggcagcgct cgcccaggcg
    30121 gtgacggagg agactgcggc gctgatgctg acaaacccgt cgacgattgg cgtctttgag
    30181 aacgagatcc accgcattgc ggaaatcctc cacgcgaagg gcgcactgct gtatatggat
    30241 ggtgcgaata tgaacgcgct gtgcgggaag acccggcctg gggacttcgg cgtggacgtg
    30301 atgcacctga acctgcacaa gaccttttcg acgcctcacg gcggcggagg cccggggtcc
    30361 gggccggtcg cgtgcaggaa gcatctggag ccgtttctgc cggtaccggt agtggaagaa
    30421 ggcaaggatg gccacctgcg gctgaactat gatcggccgc agagcgtcgg ccgagtgcgg
    30481 gcattctatg gcaacttcgg gatgtttgtg cgggcgctgg cgtatattct ggcgaacggg
    30541 ccggatggtt tgcggcagac caccgaggat gcggttctga acgcgaacta catccggaaa
    30601 aagctcgagg gcctctacga gttgccctac accacgccat cgatgcatga ggtggtgttc
    30661 agcgatcggc gacagctgcg gaacggtgtg cggaccggcg atatcgcgaa gcgcctgatt
    30721 gactacggat ttcatcctta tacggtctcg tttcccctga tcgtgcccgg cgcgctgatg
    30781 attgaaccga cggagagcga atcccgggag gaacttgatc tgttcgtcga cgcaatgcag
    30841 cagatcgccc gcgaggtaga ggagaatcct caggtggtgc tggatgctcc gcactcgacc
    30901 cgagtctccc ggctggatga gacgacggcg gcgagaaagc cgatcctgcg ctggaagaac
    30961 tcgtagtcgt taggaaaaaa gtgcttgggg tctgtgattg atgtggcctt cgtttttcag
    31021 ccgcatgatt gctggcgtgg catgccctga agggaatctc gacaacaaag tgctctaatc
    31081 gaagcgtcct ggcgcgatgc cggacacatg cgcattccgt taagatcgaa gcagatatgc
    31141 acgcgacacg cactttccct cacgctatgc tgcgtgaaat ctatgagcag ccggaagcgc
    31201 ttgcagctac gatggcgcgc tacgctccgg gcggaacgct ggatacgaac atctttgggc
    31261 ccgtggccga ggcgctgcgc gggcggcagc gcgtggtcat cgcggcaagc gggtcgagcc
    31321 ggcatgcggg cctggctggc gagatcatgc ttgaggacaa tgccggactg gcggtggacg
    31381 tggagtattc gagcgagtac atgtatcgct cgacgcatac actccacgat cctggcgtgt
    31441 tggtgatttc gcaatcgggc gaaacagcgg acacactggc gtccctgcgc gaagccaagg
    31501 cacgcgggtt gcggacggtc gcgattgtta acgatgccga ttcgacaatg gcgcgggaag
    31561 ccgatgcgtc gcttccaacg atggcgggac gcgaaatcgc cgttccagcg acgaaaagtt
    31621 ttacgacaca gctgacggtg ctgtacctgt tgtcgctgtt tctggcgcgc ataggcggaa
    31681 ggatgacggc acaaacggtt agtgcgcatt gcgagcaatt ggagcgtttg cctcatcttc
    31741 ttaatcaaac acttgaattc tgggagaagc gggtcgcggc catcgccgag aaactgtggg
    31801 aggcgcgaac cttcctgtat ctggggaggg caatccacta tgcaatcgcg cgtgagggag
    31861 ctctgaagtt gaaggagtca gcgtacgtac aggcggaggg tctgccggct ggcgaactga
    31921 agcatggccc gaatgcgctg gtgactcccg aggctccgct ggtgatgttg gcgacccggg
    31981 accacaacga tccggactcg acgctgcggt atgagaagac cctccagctc atgcgcgaca
    32041 tgcatgcgca gggggccgag atcgctgccg tgcatgtgga aggcgatgat gaggtgccgg
    32101 agctggcgca gcacgccgtt gcggtacctg cggtgaacga gtttttgctg ccgattctgg
    32161 aagtcgtgcc gctgcagatg ctggcctact tcgtcgcggt acggcgcggc gtgaatgtgg
    32221 atgcgccgcg aaacctggtg aaagccgtgg tgagggaata gggaccggcc ctccaggcag
    32281 cccgctctgc ggttcgcaga ggtgatcgat tgctgtttgg ccgcggtggc gtgcgcagga
    32341 tggggccggt ataaagaaat gaggcatggc ccaacccgct gccgcattcc tgtcccgcga
    32401 ctttcttcgc tatcaactgg cacgcgtcgt ggtgatcatc ggcgcggagg cgcaggcgct
    32461 ggcggtcgcg tggcaggtat acgaaatcac gcatcgtgcg attgatttgg ggtacacggg
    32521 gctgattctt ttcctccccg ggctggtgtt tatgctgccg gccgggcatg tggcggaccg
    32581 cttcgaccgg cggcaggtaa tcctggtttg ctatgcactg caggccgttg cgacgttcct
    32641 gttgttctgg ctggcgtgga ccagcgtcgg ccacgtttgg cagatatttg ttttgctctt
    32701 tgccatcggc accggccgcg cttttagcgg accggcaagt tcggcgctcg tgccccatct
    32761 ggttccaacg gaacacttcg tgaacgcgat cacgtgggga tcggcgattt tccagttttc
    32821 gaatattgtg ggcccggcag tgggcggcgt gttgttcact ctgccgccgc agggacggtt
    32881 gcatggagcc gccctcgtgt atctgttcac tctggcagcc attgtgtggt tcctggtatt
    32941 ggttggatcg ctgcacgtgc ggccagggcg catggagcat cgggacattt cgaaggatgt
    33001 gctgctggct ggatttcatt acgtggcgcg gacacaagtg ctgctggggt cgatttcgct
    33061 cgatctgttt gcggtgctgc tgggtggggc ggtggccctg atgccaatct ttgcgcagga
    33121 aattctgcac gccgggccgc gcgggctggg catgttgcgc gcggctcccg cattgggtgc
    33181 attgaccgtg tcgctgtggc tgatggtgcg gccaattcag cggcgggcag gggtgcgaat
    33241 gctggtgtgt gtcgcgattt ttggggcggc aacgctgttg tttggcgtct caaagagcct
    33301 gccggtgtcg ctggccgcgt tgttcatcgt gggcgcgtcg gacatggtca gcgtggtgat
    33361 ccgctcgtcg gtattacagc tggctactcc gccggagatg cgtggcaggg tgagcgcggt
    33421 gaattcgctg tttgtgggag cctcgaatga gttgggcgaa ttcgaaagcg gattgacggc
    33481 gcagtggtgg ggcgcggtgc gggcggtagt gattggcggc gtcgggtcgc tggtgattac
    33541 cggcttatgg gctgcattct ttccgagcct gcggcgcgca gacgcactga cgcctgaggc
    33601 cctgctggag gcggagaaag accatacggg acgtgaagtg cggcagctat aatgaagcga
    33661 atgtcccggt tcgtgcgatc tgccatgcat tacctttgga aagccaggaa catccccagc
    33721 ctgtcacgtg ttttgattga tggctgaagc tctggtcact ggcggttccg gttttttcgg
    33781 gggcattctc aagcgggaac tgctggctcg cgggtattcc gttacgaacg tcgacatggt
    33841 tccggacgcg gaccggcatt ctgccctgaa cagcgttcag gcagatattc gcgacgaggc
    33901 ttttctcgat tcattgtttg ccagccggcg gttcgacgtc gtgttccact gtgcggcgat
    33961 gctggcgcat ggatccatgg acgagaagct cctctggacg tccaatgtag gcggaacgca
    34021 gcgcctcgcc gacgcttgcc gaaaatatcg ggtcgagaag ttcgtcttta cctcttcaaa
    34081 ttgcctgtgg ggaaagaatc tggggcaccc cgtgcgcgag gatgaagcac cggctcccgt
    34141 ggagctctac gggcgatcga aactcgccgg ggagcaggcc ctggccgatt acacgagcga
    34201 cttcgatgtt acgattctcc gctgcccgac gatcatcagc ggcggtcggc tgggactgct
    34261 ggccattctt tttgaattca tccaggaggg aaagacggtc tgggtggtgg ggtcgggatc
    34321 gaatcgctat cagttcatct atgcgcagga cttggtgaac gcctgcatga tggcaacgaa
    34381 gacttcgggt tcggacacgt ttcatgtagg gtcggataac gtccagtcat tacgggaggt
    34441 ttacgaggcg gtgattgaac atgccgacag cccatccagg gtacgatcgc tgccgaaagg
    34501 cccgactttg gcggcgatgc atctggcgca tgtgctgagg atttctccgt tgggtcccta
    34561 ccattacaag atgatcgcgg aggacttcct ctttgatacg tcgaagatca aagaacggct
    34621 gggctggcaa ccaacgctga cgaatgcgga aatgctgatc gaggcgtacg actactacgt
    34681 cgagcggcgc gcggaaattg agagccggca ggatgtgtcg gcccatcgtc gcgcggcccc
    34741 gatgggcatc attcgtttgg tgaagtggat ctcgtgaaaa gcgtgcaagt cgagcccgga
    34801 gcggtggccg agcccagcgg atcacagttc cggtttccat ggtcgatttt ctggatcgcg
    34861 cttggagtgc ggctcgctgc gttgaccctg gggcactact accgcattgc gccgtatgaa
    34921 gaccactttg cgtttggctg ggaaatgggg cggatcgctc ggtcgctcgc aaccggtcag
    34981 ggctatgcca atcccttcga tcatttcagc ggtcccacgg catgggtaac gccgttgtat
    35041 ccgctgattc tggcagcgga cttcaagctc ttcggcgtgt atacagctgt ttcggcctgg
    35101 atgatcctcg cgttcgattc gcttctgaac gcactcatgg tgcgcacaac ctgggagatt
    35161 gcggcgcgat gcttcaatcg caaagttgcg gtctggtcgg cgtggatctg ggcggtctat
    35221 ccggcagcga tgcagtttgc cgtgaagtgg gtctgggaga tctcgctgac tgcgttcttg
    35281 tttcagtgca ttttggtcct ggcactccga atgaggaatg tcggcggggc gccggaggag
    35341 gccgaaaatg ccgcgagcac cccgcgttgg ggcgcttttg gattgctgtg gggccttatc
    35401 acgctgagca gcgcatcgat cgcgattttc ctgcccttct gcggattgtg gatcttgaag
    35461 ggggcgggaa gcctgcgacg gcagatcccg cgagtgctgc tggcctcagt tgtatgcgtg
    35521 gcatgcctgg tgccgtggct ggtgaggaat gcgctcgtct tccacaggtt cataccaatg
    35581 aggacgaact tcggcgcgga attcaatatg ggcaataacc cttaccttga gggaaccgtg
    35641 atcggctccc cggtcgcgct ggcgcatgaa gaaacgctcc tcgacaagat gggcgaggtt
    35701 caatattcga aaatgcgcgg ccgcgaagcg atggaacgga ttcgacgcga acccagcgta
    35761 tttcttgctc tcaccttgcg tcgcttctac tacttctggg ccgatgttcc gcacgccacg
    35821 atccgcggca aggagtggac cgagtatgcg cgagggctga actttgagtt tgcgagcctc
    35881 gcgggggtaa tgggattgat cctcgcggtg agacgacgcg ttccaggcgc ggtcttgttt
    35941 gcgatggcgt tcgcggtgat cccattcgtc tactactttg tttttgtgca ggcgcgattc
    36001 cgccatcccc tggagcctct gatcacaatc ctgggcgttt atctgttcca gtcggcggag
    36061 aaaagctggc gtgtaggttt gccgaggcga tagcgcctcg gttcaagcaa tgagtttcga
    36121 ttccggaagc cggttacccg agtcgtctcg gcagatcaat ccaactcggg aacgccaacc
    36181 cactccaggg cggcattacg gtcggcgaag gcgcgcatag cgaccggtgt accgttgcga
    36241 aaggtgtaaa cgtcagccag acaaacttca gtccaggcgg agctgtcttt tgtgcggaac
    36301 cgcgcgtgaa cgtacacgac aacccgatcg ccatgcacga tgaaccggac cggttcgctg
    36361 gacccctccg cccacccggc gcgggaattg gcgagatagc gcgcgacctc ggcgcgcccg
    36421 tgataggagc cgccgccggg aaattctgca ggttcctgcc agtcgatatt gaggtccagg
    36481 gcatgaaccg cggcagaaat gtcattacga ttgaaggcac tgtaggcgtc gcgcagcagt
    36541 gagatggcat gctgctgttg ctgaggcgac atagaaacct cgtagggatt ggtcaggcag
    36601 ccgcacactg cgaccgcgac gaggaggatc acagatgcca tgcacctatt ttgacgaaac
    36661 cagctcctga tgcgcaaaaa agtgactgat tagcccagcg gaaggaaata ttgggttccg
    36721 tgaacgggtt ctgccaggcg tttgagcagg agctggaggt cccggttgct attcacaaag
    36781 tcgatgtcgc tggtgttgac gacaagcaaa tcgctcgctg agtagtggaa gaaaaagtgc
    36841 tcataggcgg ctgcaacctg ctcgatgtat ttctcgctga tgaagcgttc gcccgggacg
    36901 cctttgcgac gaatgcgctg gttcagagct tccggtgatg cctgaaggta aatcaccaat
    36961 tcaggagtgg gaacatcagc acgaaacatc tggtagcagc gattgtagag gtcgagctcc
    37021 gcatcggaga ggttgatata ggcgaagatc ttgtcttttt cgaacaggta gtcggagacg
    37081 atgggggtgg tgaaatcctc aagttcccgc tgctgctcgt agcgctccat caggaaccac
    37141 atctgggtgg cgaatgcgag gccaggctcg ccgcgataaa agcggtccag gaagggattg
    37201 ttctcaggtt cgacaagacg gcgggcctgg agagtttctg cgaggatgcg ggcgagcgag
    37261 gatttgcccg cgcgaatggg gccctcgacg gcaataaagc gcggctgttc gaagagattg
    37321 gccacgagtc agaaacgtgc agcgaaggaa acacgaacat atcatgggct gagcgggatg
    37381 caaacccgca tacactggaa agcaaatgat cggagttctt gccagtgtcg caggcgcagc
    37441 cggcctcagc gccggagtct ggacttacgg gtccctggcg ccggaatcgc agctgtttgg
    37501 gcgaacgatt gtggcagggc ccaatgcggc ggagtttgcg ctgacgttcg atgacggtcc
    37561 caacgatccc tggacgggac gtttgctgga tgtgttggct cgctatcagg tccgggctac
    37621 cttttttctc atcgggcgat tcgtgcggca gtgccccaaa gtcgtgcggg aaattcggga
    37681 tgcggggcac tgcatcggca atcacacggt gtctcaccca tggctggctg tgcagagtgg
    37741 agcgcgagtc cgcgaggaac tgtccgggtg caacgtggcc ctggaggatg tgttgggcga
    37801 gaaagtgcag ttctttcggc caccgcacgg ctcgcggcgg ccggcggtcc tggatacggc
    37861 gcgggaactg gggctgactg ccgtgatgtg gaacgcaatg ggattcgact ggcgcgcgaa
    37921 tataacggcg gcacagatta cagagcatct ggagcaggga attgcgcgga accagcggcg
    37981 aaagcggggt tcgaatctcc tgctgcatga cggtggacat gcggcgttgg gagcggaccg
    38041 ctcccaatcc gtggaggcga cgcggaacat cctcgagagg catcctcctg gcacggttcg
    38101 atacgtgacc gcggatgcct gggtatgagt tgtcccattc gtcccagaat acgaccggaa
    38161 ctccttgctt tgcgataacg tagggaaatt cagagaggct ttccgcgccc atgtttgaag
    38221 acattcgcta tgccgtccgg cagtttctga agacccccgg cttcaccatt acagcgattc
    38281 tcaccctggc cttaggaatc ggtgcgacga cggcaatctt cacgctgatc aacgcggtcc
    38341 tcctcaagtc tttgccggtg gcgaatccga gcgaactgat caggatcggc gacaacgaaa
    38401 actgctgcat caacggcggc atgcaggatg actggtcgct cttttcgacc gagcagtacc
    38461 ggcgattccg cgaccacaca cccgggttct cgaaccttgc ggcatttcag gcgggacgga
    38521 cacaggtagg agtacggcgc gcgggcagcg atcatccggc ggaatcgttt ggggctgagt
    38581 ttgtctcggg caatgcgttc gagacatttg ggctgactcc atggatgggc cggctgctcc
    38641 gaccgtcaga cgatgtgaag ggagcaccgc tggtcgcggt gatgagttat cgcacctggc
    38701 agcaaaaatt cggcaaggat ccgtccgtag tggggacatc gtttttcgtc aacggcaacc
    38761 ccttcaccat cgttgggatt ggacctcccg gttattttgg cgagcggttg acgacagatc
    38821 ctccatcgtt gtggatcccg ctcaacga
//
LOCUS       DASZYN010000247         1722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1230555, whole genome shotgun sequence.
ACCESSION   DASZYN010000247 DASZYN010000000
VERSION     DASZYN010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230555"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1311)
                     /locus_tag="VHE33_06410"
     CDS             complement(184..1311)
                     /locus_tag="VHE33_06410"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HVT97121.1"
                     /translation="MSFLERLNALIWFLLAAAWFLFSDLIAQHAAEGLDSGDFQEPLY
                     RIFLLFLLIVGYWVMSRLGQRRMISAKATALAPRPGWGREFALGAALGWAGVLACILP
                     VALIGGLVITVFTNAHQWLVLLLDLIALAAGTLAIEVAFRGYAFLRLVEAMGPVLGAF
                     FMAAVYAIWRTHAAPTTTAAVLVSFFLGLVLAIAVLRTRALWVSWGFHFAWLASMSVL
                     FGLPVSGSMNYSPILVTNARGPAWISGGGEGPEGCAFAVLVSFLLMFFMARFTSELKH
                     KYGYPEIVPGGMPVDIDAAARAQHEAAMGAAEPQQPPLVQIQPAQPVAANSENPFVAE
                     TVTSAEPNAVEAPAPDFDATPPRETEPEPPPPNTDSQDSKL"
     gene            complement(1381..>1722)
                     /locus_tag="VHE33_06415"
     CDS             complement(1381..>1722)
                     /locus_tag="VHE33_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97122.1"
                     /translation="GLAVADRVLIFENAIYSVIPPEGCAAILWRDAAFKDKAAEAMRI
                     SASDVRSLGCVDDVIPEPEGGAQSDPEKAAEFLAEKLRWHLNELKALSIDELLRRRYD
                     KFRNIAQFYTV"
BASE COUNT          369 a          458 c          631 g          264 t
ORIGIN      
        1 gaacaaacga tgcttcggct tcgccggcaa ctgggtaacg aggctttcag caagctggac
       61 acattcgtca gccatcgcga aagcggcggg agaagcgtgg atcgagggcc aatccgcaaa
      121 ggtccggtgg aaacggcgca aagcagaggg acgccatccc ggccatagcg gttccggcgc
      181 tcgctacaat ttcgaatcct gcgaatcggt gttcgggggc ggaggttcgg gctccgtctc
      241 acgcggtggt gttgcgtcga aatccggcgc tggcgcttcc actgcatttg gttcggcgga
      301 agtcacggtt tcggcgacaa acggattttc agagttcgcc gcgaccggtt gcgcgggttg
      361 aatctgtacc agcgggggct gctgcggttc agccgcgccc atcgcggctt catgctgcgc
      421 gcgggcggct gcgtcaatgt ctaccggcat acccccgggg acaatttccg ggtaaccgta
      481 tttgtgtttg agttcgctgg tgaagcgggc cataaagaac atcagcagga acgagacgag
      541 cacggcgaag gcgcagccct cggggccttc tccgccaccg ctgatccagg cgggtccgcg
      601 cgcgttggtg acgaggatag gggagtagtt catggagccg gaaaccggca ggccgaagag
      661 cacgctcatg gaagcgagcc aggcgaagtg gaatccccag gagacccaga gagcgcgggt
      721 gcgcaggacc gcgatcgcga gcacaagtcc caggaaaaaa gacacaagga cggcggcggt
      781 ggtggtggga gcggcatgcg tgcgccagat ggcatagacg gctgccataa agaaggcgcc
      841 gaggacgggg cccatggctt ccacgagacg gaggaaagcg tagccgcgga aggcgacttc
      901 gatggccagg gtgccggcgg cgagggcgat gaggtcgaga agcagcacca gccactgatg
      961 cgcgttggtg aagaccgtga tgacgagtcc gcctatgagc gcgacgggaa gaatgcaggc
     1021 gagaacgcca gcccagccca gagccgcgcc gagggcaaat tcgcggcccc atccggggcg
     1081 aggagcgagc gcggtggcct tggcggagat catgcggcgc tgaccgaggc ggctcatgac
     1141 ccagtagccg acgatgagga gaaagaggag gaagatgcgg tagagcggct cctgaaagtc
     1201 gccggagtcg aggccctcgg cagcgtgctg cgcgatgagg tcggagaaga ggaaccaggc
     1261 ggcggcaagg agaaaccaga tcaacgcgtt gaggcgctcg agaaagctca cgcggttgct
     1321 ccgaacggaa atgcggcgcg ctggaaagaa gtcatcaggg ctatggatgc caggctggga
     1381 tcagacggtg taaaactgcg cgatgttgcg gaacttgtcg tagcggcggc gcaggagttc
     1441 gtcgatggag agagccttca gttcgttgag atgccagcga agtttttcgg cgaggaactc
     1501 ggcggctttt tcgggatcgc tctgcgcacc gccttcgggc tcaggaatca cgtcatcgac
     1561 gcagccgagg gaacgaacat cggaggcgga gatgcgcatg gcttcggcgg ctttgtcttt
     1621 aaaggcggcg tcgcgccaca ggatggcggc acagccttcc ggcggaatga cggagtagat
     1681 ggcgttctca aagataagaa cgcgatcggc gacggcgagt cc
//
LOCUS       DASZYN010000248         6640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_329274, whole genome shotgun sequence.
ACCESSION   DASZYN010000248 DASZYN010000000
VERSION     DASZYN010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329274"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /gene="modA"
                     /locus_tag="VHE33_06420"
     CDS             <1..406
                     /gene="modA"
                     /locus_tag="VHE33_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HVT97123.1"
                     /translation="LDGLRNPTVKSIAIANPEHAPYGRAAQATLQHAGLYDILRPKLV
                     IAENIAQAAQYADSGNAQAGFISLTSALTPRLRASGRFVRVPSEDYPPILQGAIVIRN
                     SPGAAAAHRFLDFLAQPAIRQSLAAQGLEPAR"
     gene            407..1075
                     /gene="modB"
                     /locus_tag="VHE33_06425"
     CDS             407..1075
                     /gene="modB"
                     /locus_tag="VHE33_06425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HVT97124.1"
                     /translation="MDLQALGLTLRLAAITTVVLLALGIPLAYWLASAHTRLRPLVEA
                     FTTLPILLPPTVLGFYLLVLLGPRTAFGRATAHLLGHPLAFSFSGLVIGSVLYSLPFA
                     VQPLAAGFRSVDTALLEQARLLGAGRLRTLTSVLLPLSTHSVIAAAALCFTHTIGEFG
                     VVLMIGGDIPGATRTLSIAIYDQVQEFSYAAANRTSLVLIAVAVLALVLLYARRSNKE
                     ASRG"
     gene            1068..1775
                     /locus_tag="VHE33_06430"
     CDS             1068..1775
                     /locus_tag="VHE33_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVT97125.1"
                     /translation="MADIEIRHRQGRFTLDASFSLPGAWTVIFGPSGAGKTTLLRVLA
                     GLSCPTSGRIRFEGRTLLDTAANIDVPAGKRSIGFVTQQPALFPHLTARQNIAFGIQH
                     LDRDRRTARVDEMLQLFGAESLADQPAPQLSGGERKRVALARALAPAPDLLLLDEPLA
                     GLDDTSAHDILARLLSLQGSAQVRIAYVSHDLAEIWRLPAEVVCLESGCVTAVGPLQQ
                     VLASHRDRLLQQLRPPS"
     gene            complement(1875..3506)
                     /locus_tag="VHE33_06435"
     CDS             complement(1875..3506)
                     /locus_tag="VHE33_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HVT97126.1"
                     /translation="MLVRGKFLGAALCSLCLVFAAAAKADDLTIKTDKGKVHGKKSDD
                     GQVRMFLGIPYGAPPVGPLRWKPPQPAAKWAGEREATDFGSRCMQPDIYHDMHFRDPG
                     PSEDCLNLNVWTPAKDKHAKLPVMVWIYGGGFVAGAASEPRQDGQYLAHKGVVVVSMN
                     YRLGLFGFFAHPDLAAESPQHAAGNYGLMDQTAALEWVKKNIAAFGGDPKNVTIFGES
                     AGSFSVSAQMASPLAQGLFERAIGESGAAFNGRGLSFAPLAEREKADPAWALATFGTS
                     DLKALRAMSAQDLMEKMATAAAAASAAAPGTPRVRGGDRPDIDGYFLPESVPQIFKEN
                     KQAHVPLLAGWNRDEPSALAVNQPAPPTVASFQEMAQKDFGDRATDFLKVYPGTTDAE
                     AVRSAIDYMGARFLTFATWEWLDAQVTDGGAAYRYHFERPSPSDKYHPAGSGAFHSDE
                     IEYVFGTLDSRPEAVWQPEDHQLSDLMQTYWTNFARTGDPNGGDVPKWPQYKGDDGWQ
                     VMHLDADSHAAPDDHRDQWLFLQGAWATQGAANSQ"
     gene            complement(3566..4795)
                     /gene="lysA"
                     /locus_tag="VHE33_06440"
     CDS             complement(3566..4795)
                     /gene="lysA"
                     /locus_tag="VHE33_06440"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HVT97127.1"
                     /translation="MSVEGVSMDQLARRYGTPLYVYSAGHVLERFELFAQAFSGVPHL
                     VCFAVKANPALAILKMLAQKGCGFDVVSGGELERVLTVDRAAAERVVFSGVGKTAAEM
                     DLALRAGILMFNVESEEELTLLAERAARRRRKARFALRVNPDVFAETHPYISTGMREH
                     KFGIDIQRARAVYRKAGSYLEAAGVSVHIGSQIRSADPFGAAAERVVRLIADLRRDGH
                     AIRYVDLGGGLGVEYHLDKAFDAAEKVRQYAAALISHVQKAGGLTLLLEPGRFLVAQA
                     GALVARVLYVKKNGAKTFVIVDAAMNDLIRPALYQAHHEIVPVVKPRSGKKAVVDVVG
                     PVCETGDFLARDREMPVVRPGDLVAILDAGAYGMSLASNYNSRPRPAEVLVRGRRAQL
                     IRRRETIQDMLQAERRS"
     gene            complement(4942..>6640)
                     /locus_tag="VHE33_06445"
     CDS             complement(4942..>6640)
                     /locus_tag="VHE33_06445"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HVT97128.1"
                     /translation="ITGEVSPSGTMPVIQLGTLSAGSSVTQNVVIGDAGWAESGDDGT
                     EGSPARVPVTGEWIGRFTGYAHSAWFEWWARGGREFTVEAVALDGDGNPTENKAQVVI
                     GAWNGTDVPGSAPVTGTVQPLNGHEAGLTTLPVLTIADSEVRIGLADGRGDGRPDYVY
                     RGRVLYADSVTPARLPADGGPMLIRGMGFRRGVTVTVNGVAAPVSDVTPTTIVATAPA
                     SGGATGTAIIQVQDPQTLGIAAINSGVSYDAAADDAIGLMTAPMGMVPIGVPQPFTVR
                     TINVTSQTAAAGVTVTFAVTQGAATLGCGQSSCNVITGEDGVAMLLVTPKTAAQAQVV
                     ASLANGSSVTAEFSGTTPPAIAALTPNLYVTLGATVQWPVQALVLNGAGGALAGQSVV
                     WTPDSGISVAYGQSVSDASGIASNQISAGPFSASANAIASACLAGSNNCATFTVTPVH
                     KETAVLAPWSGTSQFIAAGQTFAPLVVRVTDPFGHPLAGANVTFAEMLTGWTQPCPVQ
                     GTCPPAPVLGEQIVEATSGIDGLVRLTPLSGSGLAARLLVMAATGDATLTFELDQHP"
BASE COUNT         1185 a         2524 c         1688 g         1243 t
ORIGIN      
        1 cctcgacggc cttcgtaacc caacagtcaa atcgatcgcc atcgccaatc ccgagcacgc
       61 cccctacggc cgcgccgcgc aagccactct ccagcacgcc ggactctacg acatcctccg
      121 ccccaaactc gtcatcgcgg aaaacatcgc acaggccgcg cagtacgccg attccggcaa
      181 cgctcaggct ggtttcatct cactgacctc cgcgctcacc ccgcgccttc gcgccagtgg
      241 ccgcttcgtc cgcgttccct ccgaagacta cccgccgatc ctccagggag cgatcgtcat
      301 tcgcaacagc ccaggcgctg ccgccgcaca ccgattcctc gacttcctcg cgcagcccgc
      361 aatccgccaa tcgctcgcgg ctcagggtct cgagcccgct cgttagatgg atctccaggc
      421 gctcgggctc acgctgcggc ttgcggccat cacgaccgta gtgctgctgg ccctcggcat
      481 tccgctcgca tactggctcg cctcggcgca cacgcggctg cgcccgctcg tcgaagcctt
      541 caccacgttg cccatcctcc taccgccgac cgtcctcggc ttctacctcc tcgtcctcct
      601 cggccctcgc accgctttcg gccgagccac cgctcacctc ctcggtcatc cgctcgcttt
      661 ctccttctcc ggacttgtta tcggttccgt gctctactcg ctgcccttcg cggttcagcc
      721 gctcgctgcc ggattccgct ccgtagacac cgcccttctc gaacaggccc gtcttctcgg
      781 cgccggccgt ctccgcacgc tcacctccgt tttgcttccc ttatcgaccc attccgtcat
      841 cgccgcagca gcgctctgct tcacccacac catcggcgaa ttcggtgtcg tcctgatgat
      901 tggcggcgac atccccggcg ctactcggac cctctccatc gccatctacg atcaggtcca
      961 ggaattctcc tacgccgcgg cgaaccgaac ctcactcgtg ctgatcgccg tcgctgttct
     1021 tgccctcgtg ctgctctacg ctcggcgctc caataaagag gcgagccgtg gctgacatcg
     1081 agatccgtca ccgccagggc cgtttcaccc tcgatgcatc gttctctctt ccgggagcct
     1141 ggaccgtcat cttcggtccc tccggggcag ggaagaccac tctccttcgc gtcctcgccg
     1201 gcctcagctg tcccacttcc ggccgcattc ggttcgaggg acggactctg ctcgacaccg
     1261 ccgccaacat cgacgttccc gcgggcaagc gctccatcgg attcgtcacc cagcagcctg
     1321 ccctctttcc gcatctcacc gcgcgccaga acatcgcctt tgggattcag cacctcgatc
     1381 gcgatcggcg cacagctcgt gtcgatgaaa tgctgcagct ttttggagcc gaatcgctcg
     1441 ccgatcagcc ggcgccgcaa ctctccggag gcgagcgcaa acgcgtcgcg ctcgcacgcg
     1501 ccttagcgcc cgctcctgac ttgcttttgc tggatgagcc cctggccggt ctcgacgata
     1561 cctcggcgca cgacatcctc gcgcggttgc tctcgctcca gggctcagct caggtccgca
     1621 tcgcctatgt ctcgcacgac ctggccgaga tttggcgtct tcctgccgaa gtggtttgcc
     1681 tcgaatccgg ctgcgtcacc gccgtcggcc cgctgcagca ggtcctcgca tcccacagag
     1741 accggctact ccagcagctc cgcccgccct cctgagcaag tttccgaatt agtcgcgatt
     1801 ctcctgccgc tacaaggatc tcggacccca atcgaagaac ctgtggttcg cattggctgg
     1861 ctactggcta ctggctactg gctattggct gctccctgcg tcgcccacgc cccctgcagg
     1921 aacaaccact ggtcccgatg atcatccggc gccgcatgcg aatccgcgtc cagatgcatc
     1981 acctgccacc catcatcgcc cttgtactgc ggccacttcg gcacatcgcc gccgttggga
     2041 tcgcccgtgc gcgcgaagtt cgtccagtaa gtctgcatca gatcgctgag ttgatgatcc
     2101 tccggctgcc acacggcctc cggtcgcgaa tcgagtgtcc cgaagacata ctcgatctca
     2161 tcggagtgga acgctccgga tcccgcagga tggtatttgt ctgatggcga agggcgttcg
     2221 aagtgatacc gatacgcggc gccgccatcc gtcacctgcg catccagcca ctcccacgtc
     2281 gcgaaggtca gaaaacgcgc acccatgtag tcaatcgccg aacgcaccgc ttccgcatcc
     2341 gtcgtccccg gatacacctt cagaaagtcc gtcgcgcgat ctccaaaatc tttctgtgcc
     2401 atctcctgga aactcgcgac cgtcggtggc gccggctgat tcacggccag cgcgcttggc
     2461 tcgtcccgat tccaccccgc cagcagcggc acatgcgctt gtttgttctc cttaaagatc
     2521 tgcggaacgc tttccggcag gaaataccca tcgatatcag gacggtcgcc gccccgcacg
     2581 cgcggtgtgc ccggagccgc ggctgatgcc gctgcggctg cagtggccat cttctccatc
     2641 agatcctgcg cgctcatcgc acgcagcgcc ttcaggtccg acgtcccaaa cgtcgccagc
     2701 gcccaggccg ggtcggcctt ctcgcgctcc gcgagcggag caaaggagag tccgcggcca
     2761 ttgaacgccg cgccgctttc gccaatcgct cgctcgaaca atccctgcgc cagcggtgaa
     2821 gccatctgcg cgctcaccga aaacgatccc gccgactctc cgaaaatcgt tacgttcttt
     2881 ggatccccgc caaacgccgc gatattcttc ttcacccact cgagcgcggc cgtctgatcc
     2941 atcaatccat agttgcccgc ggcgtgttgc ggcgactccg ccgccagatc aggatgcgcg
     3001 aagaatccga acaatcccag ccggtaattc atcgacacga ccactacgcc cttgtgcgcg
     3061 agatactgcc catcctggcg cggctccgac gccgctcccg ccacaaaccc tccgccgtag
     3121 atccacacca tcaccggcag cttcgcatgc ttgtctttgg ctggcgtcca cacgttcaga
     3181 ttcaggcaat cctcgctcgg ccccggatcg cgaaaatgca tgtcatggta aatatccggc
     3241 tgcatgcacc gcgagccgaa gtccgtcgcc tcacgctctc ccgcccactt cgccgcgggt
     3301 tgcggcggct tccagcgcag cggccccacc ggcggtgctc cgtaaggaat ccccaaaaac
     3361 atgcgaacct gtccgtcgtc gctcttcttg ccgtgaactt tgcccttatc ggttttgatc
     3421 gtcaggtcat ctgccttcgc tgctgctgcg aagaccaggc acagggagca cagcgccgca
     3481 cccagaaatt tcccacggac aagcatcggt cgcctccgcc gccaaaccgc aggggtttgg
     3541 gcagacgaac caaaatacca gaaggctagc ttcttcgttc cgcctgcagc atgtcctgga
     3601 tggtttctct gcgtcgaatc agctgcgctc tgcgccccct caccagcacc tctgcaggac
     3661 gcgggcgcga gttgtagttc gacgccagcg acatgccgta ggcgccggca tccagaatcg
     3721 ccaccaggtc gcccggtcgc accacaggca tctcgcgatc gcgcgcgaga aaatcacccg
     3781 tctcgcaaac aggccccact acatcgacga ccgctttttt cccggatcgc ggctttacca
     3841 ccggcacgat ctcgtgatgc gcctgataaa gcgccggccg aatcagatcg ttcatcgccg
     3901 catcgacaat cacaaaggtc ttcgcgccat tcttcttcac gtacagcacc cgcgccacca
     3961 atgcacccgc ctgcgctacc agaaaacgcc ccggctccag cagcaacgtc agtcccccag
     4021 ccttctgcac gtgcgaaatc agtgccgccg catactgccg caccttttcc gccgcgtcga
     4081 acgctttatc caggtgatac tccacaccca ggccgccacc caaatcgacg taccgaatcg
     4141 catgcccgtc ccgccgcagg tctgcgatca gccgcaccac tcgctcggcg gccgccccaa
     4201 acggatcagc actgcgaatc tgcgatccga tatgaacgct caccccagcc gcctccagat
     4261 agctgccggc tttccgatac acagctcgcg cgcgctgaat atcaatcccg aacttgtgct
     4321 cccgcatccc ggtggagata taaggatgcg tctccgcgaa gacgtccgga ttcacccgca
     4381 gagcaaaccg cgccttccgt cgccgcctcg ccgcccgttc agcgagcaac gtcaactctt
     4441 cctcgctctc cacgttgaac atcagaatgc cggcacgcag cgccagatcc atctccgccg
     4501 ccgtcttccc aaccccggaa aacaccacgc gctccgccgc cgcgcggtcc accgtcagca
     4561 cccgctccaa ctccccgccc gacacaacgt cgaacccgca gcccttctgc gccagcatct
     4621 tcaggatcgc cagcgccgga ttcgccttca ccgcgaagca caccagatgc ggaacccccg
     4681 agaacgcctg agcaaacagt tcgaatcgtt ccagaacgtg tccggcggaa tacacgtaca
     4741 gcggtgtccc ataccggcgc gccagctgat ccatgctgac gccctcaacg gacaagacct
     4801 tttcagattc ggaatagaag aacgaacgag taggaatcct gagacctcaa cacaaccaat
     4861 tttccctgag actatctgcc ttccgcactg gcgtaaagcc gcggccctgc cgcaacatcc
     4921 cctgacgaga gcagggtagc ttcacggatg ctgatcgagt tcgaacgtca gtgtcgcgtc
     4981 gcccgtcgcc gccatcacca gtaaccgcgc cgccagtcca ctcccggaaa gcggcgtcaa
     5041 ccgcaccagg ccgtcaatgc cggacgttgc ctccacaatc tgttcaccca gcacaggtgc
     5101 cggcggacat gtcccctgta ccggacaagg ctgggtccat ccagtcagca tttccgcaaa
     5161 agtcacattc gcgcctgcca gtggatgccc gaaaggatcc gtaacccgga ccaccagcgg
     5221 cgcaaatgtc tgccccgcgg cgatgaattg cgacgtccca ctccaggggg caaggaccgc
     5281 cgtttccttg tgtactggcg tcacggtgaa tgtggcgcag ttgttgcttc ctgcaagaca
     5341 cgcgctcgcg atggcgttgg cacttgcaga gaaaggccca gcagagatct gattgctggc
     5401 gatcccgctc gcatcgctga cactttgccc gtacgcaacg ctgatcccgc tgtcaggagt
     5461 ccaaacgacc gattgcccgg ccagcgcacc accggctcca ttgagcacca gcgcctgcac
     5521 cggccattgc acggtcgccc ctaaagtgac atacagattc ggcgtcagtg ccgcaatggc
     5581 aggcggtgtc gtccccgaaa attcggccgt cacgctgctc ccgttcgcca gcgaagccac
     5641 cacttgcgcc tgcgccgccg tcttcggagt aaccaacagc atcgcaacgc catcctctcc
     5701 ggtgatcacg ttgcacgaac tttgtccgca acccagcgtg gccgccccct gggtcactgc
     5761 gaatgtgacc gtgaccccag ccgccgcagt ctggctcgta acgttgatcg ttcgaactgt
     5821 gaacggctga ggcactccaa tcggaaccat ccccatcggt gctgtcatca gcccgatcgc
     5881 gtcatccgcc gccgcgtcat agctcacgcc gctgttgatc gccgcgatgc ccaacgtctg
     5941 cggatcctgc acctgaatga tcgcggttcc cgtggccccc ccggatgccg gagccgtcgc
     6001 cacaatcgtt gtcggcgtca cgtcgctcac cggcgccgcg actccgttga ccgtcaccgt
     6061 cacgccgcga cgaaaaccca ttccccggat gagcatcggt ccgccatccg ctggcaatcg
     6121 cgccggtgtc acgctgtccg cataaagaac gcgtccccga tatacgtaat cgggccgtcc
     6181 atcgccccgt ccatcggcca ggccgatccg cacctcgctg tcggcgatgg tcaacaccgg
     6241 cagcgtcgtc aaacccgcct catggccatt gaggggctgc acggttcccg tcactggcgc
     6301 gctccctggc acatcggtcc cattccatgc gccaatcacc acctgcgctt tgttctccgt
     6361 gggatttccg tccccgtcca gagcaaccgc ctcaaccgta aactcgcgcc cgccccgtgc
     6421 ccaccactca aaccacgcag aatgcgcata gccggtgaat cgtccaatcc attcgccggt
     6481 gacaggaacc ctcgccggcg atccctcagt tccatcgtcg ccgctctcgg cccagcccgc
     6541 gtctccgatc accacgttct gcgtcacact cgaaccggcg ctgagcgtgc ctaactggat
     6601 caccggcatc gttccggaag ggctaacctc gcccgtgatg
//
LOCUS       DASZYN010000249        17636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_521870, whole genome shotgun sequence.
ACCESSION   DASZYN010000249 DASZYN010000000
VERSION     DASZYN010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 17636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17636
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521870"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /gene="purE"
                     /locus_tag="VHE33_06450"
     CDS             complement(<1..413)
                     /gene="purE"
                     /locus_tag="VHE33_06450"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HVT97129.1"
                     /translation="MREKPLVGVVMGSKSDYETLAPAVEILREFAVPHEARVVSAHRT
                     PNLLFEYASQAEGRGLRVIIAAAGGAAHLPGMLAAKTLVPVLGVPIPATMLQGVDSLL
                     SIVQMPKGVPVGTLAIGRPGAANAALLAIEILATT"
     gene            complement(458..676)
                     /locus_tag="VHE33_06455"
     CDS             complement(458..676)
                     /locus_tag="VHE33_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97130.1"
                     /translation="MTDFEAQVLADLSVLKNQMVTLVGDGNSGRIGVIEQRVTQHEER
                     FQSARGFAIAIGGLVTLIQFVVDFLRRK"
     gene            complement(758..3091)
                     /locus_tag="VHE33_06460"
     CDS             complement(758..3091)
                     /locus_tag="VHE33_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97131.1"
                     /translation="MKLTIDNHDGNGPIDYTAAIVAEQPFRIVRRLNQPSTCVVSLFP
                     AHGLVTPVRHARMIVANDDGTLLFTGYIVTEPAMEFVGRGTEGFVWQAAISAISDEIL
                     LNQQALPGLATICGASSGEALQALLAAVQFGKISFDLSQSNLNVSQFQPTESGTWSEN
                     AGELAASVRSAYRLINGALTLLPVGSITHSLSESDGTLSLRGLAMSRVRALANDVTVC
                     GEIEPCVYVTEFFQADGTSVLFNLSERPWMPAASKSKPLTDNFQGSTIDTRIWNVDDP
                     GASLALTSAGLTCGGGGSSIGATVVSAISSLEMGGGFVAEAGGVQFGANTSGIVNGFF
                     TAGQATVISCIAGFLIGQANAATTISPLVNGAIAGSTFTAVAGHLYTLRLRFSSSEVQ
                     RALQAYYSVAPDSGLERFGADLLDAAANIIFEVQDTTNGIASTPVVLYSGEFTSPPAP
                     WCLYAPLVAGYLQCSLGDVSVERQGPVWVTTTSANGTPVVRRIGTPAQGADCTINGDG
                     MLQFYPLSTPQTGDVIAVSYRTSRRSVARLANASSIDAESNGGTLPGTACWAGTVTRP
                     APRSSADCENAAAAILAVSTDRAAAWKGTYSEWNADEQGDVWPGDQLAITSPSAGLST
                     SLVVREVQIEMLSGAPVRTKYAISFANDWAEEISIQTSSTLPADAWLVRQPEAATPLA
                     NLISLAVTSITGSAIQIDTGVTPPAGGGFEVRRRDWSFAAGSGPDLVLRSPVPNFIIP
                     RQGAMERYYIRMYDGSATPNYSRFSSAVFVNLPLTHS"
     gene            complement(3106..4086)
                     /locus_tag="VHE33_06465"
     CDS             complement(3106..4086)
                     /locus_tag="VHE33_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97132.1"
                     /translation="MSTQTTTPLPALPPMGRSVRAFFAPVDRVHRLPTVFDPSVNGRF
                     ATNAPPAPWIDLGWIEDFSRKSTSKYAGLATGSPAATQFQVRETVDATVSLRFKAWSK
                     LSMALAAGSQHMNLIVPAPGSVANGSGSKGLLPVNLMAGSTPTFLAMAASDAAQFAAG
                     SLITVDDDYAGQIGFVGSGVFAAYVASAAGVNNDADYIRRVSFNVTRVASSTSAGLQL
                     AQPLIAGAPTATMRVQPISGFVDREGGSFFQEWSALFVIDGEQGERILFHYPRLQTMA
                     GAAEAATTLAPPLERVALSATFRALPVTDANDGEQVVCFRTFLPSPATLI"
     gene            complement(4083..4574)
                     /locus_tag="VHE33_06470"
     CDS             complement(4083..4574)
                     /locus_tag="VHE33_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97133.1"
                     /translation="MMQNAKDTFYIALRSRLASMNPARTMTLRAVTRPGILVTEAEAP
                     AQQPFLDTFALNWTEMRADLQLPAILAQMTCEIQYSTAGTQARAGLDRGRALEEMDYE
                     VLQLLYPYSAQKMNYTTTPAVGMETMVFWSEPEFGPAVTQRDCLSRTVKVTVFAFQEQ
                     GEQ"
     gene            complement(4643..5083)
                     /locus_tag="VHE33_06475"
     CDS             complement(4643..5083)
                     /locus_tag="VHE33_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97134.1"
                     /translation="MADRSVQNSSGFARRLATAMLRTGGGTTACLQMPPLMGDSTDAG
                     QIGLNAPGFLSLPLAPVVFRKEREALQDGQRTKYELLISADAVAAQVTQLQLSSAQAL
                     FAMASGVVIAGKGFLIEAISASEHQGQAYLYRLLLQEASGEWPM"
     gene            complement(5087..5713)
                     /locus_tag="VHE33_06480"
     CDS             complement(5087..5713)
                     /locus_tag="VHE33_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97135.1"
                     /translation="MACYLQPTEYQTYGLASDVTDDWITAASSLMDAHCRRASLSVTQ
                     YTERLRIVEGSQSARLTYLPLAPLAQATQTFVSIQARYARPRRGEMAYPQLEEALWAF
                     SLPGAWTAVDPASVDFVAETGELTLPLNLLGLSYNEIEATYTAGLASLPSPVKCACAQ
                     IVKNAQATPGLNVKSSRVDTLQLQYFSGSLIDATVQALLRPWVSVKVG"
     gene            complement(5865..6833)
                     /locus_tag="VHE33_06485"
     CDS             complement(5865..6833)
                     /locus_tag="VHE33_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97136.1"
                     /translation="MTANFLDIHAAADYIGPGAVEVPLYQTEITDIVRRRNLFGQRIK
                     QVPATGHPSRYFEQTAIPNPSTAGFVDPRNIVAPVVTPTRVERSVPLKAIVAQLNYNL
                     FDMELGAQQKQFAYLQAKDLVDTIEGVMLAHDVSLWNGNDTSLSAPTTMQYFGVAGQI
                     AAGGNTTTIGTSASITDGLKSTIAQMCANTSYAVRPTAIYANPVLLDLIDREMKSEFN
                     VILNTKEIAAGFTVKMLTTQAGELPLIPEWSLAYTGTPGSGSAVLPAYIVTEDLIEYH
                     WLSDPNPRVFQLGTPGSLASQHVVVKFGAPVVKGASYAHYQVLVDR"
     gene            complement(6916..7353)
                     /locus_tag="VHE33_06490"
     CDS             complement(6916..7353)
                     /locus_tag="VHE33_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97137.1"
                     /translation="MEAQAGENMVALTDSMERMEARLEAAAALFERAAALIEQQESER
                     TGEVRKIVAAVETSREAELQKKLEAAEQTITELRAQMEARAEAPKSGRKTVSASTAQL
                     LAKQGISSLDSLEAGALDAALHGLSLEQRIAVKAQLLRAGVVS"
     gene            complement(7317..7991)
                     /locus_tag="VHE33_06495"
     CDS             complement(7317..7991)
                     /locus_tag="VHE33_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97138.1"
                     /translation="MTGDIMKLEAMAVTLPEVAGHPNRAPFSGVLTLVDEPSTKPPSG
                     ARGHRVILTRGAALSALPSLLGMAVDYVPSWDGHDSRRKCGIITHADVDGSRLRVAGY
                     LFAKDFPEVERQMHECLPGAMGMSWELADAHVEDMRAEIWTLTRATFTGAAILLREKA
                     AYRNTSFELAARSCRSIIARSALRERVHAGTNRRIPEAAGPARPVRTTGKEETWKHRQ
                     AKTWSR"
     gene            complement(7988..9307)
                     /locus_tag="VHE33_06500"
     CDS             complement(7988..9307)
                     /locus_tag="VHE33_06500"
                     /inference="COORDINATES: protein motif:HMM:NF016732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HVT97139.1"
                     /translation="MRIREQWKHAMALLGAGRREETAQRKTVALPSVLAPYSFPAKAL
                     PKPTPANLRKFAETPVVRRAINLIKDRVAAMDWQVRLKRDCEPGREAGSAERARILRR
                     SLEFPNPSDSFRTLLEQTIEDALVGGFGAIEMELTGDENQPFELWAVDGATIRIDPKW
                     DGSAESVRFAQATGRLGTDAVIPLRDNQLIYVRMNPRSYTPFGLGPLEVAFETVNSFL
                     SAHRYAGKLASNAVVQYALWLNETTPVQHERLIRWWQDEIEGTGRVPLLSTEQKPEVL
                     RFAAGTDADLRLSWQEFLIRMIANAFSLPPLLLGLEQDVNRSTASELTEEAFHSAIVP
                     LARLVAEHLTRDLFAKRLGWPEFEFVFNDLETSDEMQEVQMQTALLAAGVLTVNEVRA
                     MRGLGPMAAESGLAGNGTTSQGGGSTVQGTEKEENVTTTITDQEMQG"
     gene            complement(9414..10349)
                     /locus_tag="VHE33_06505"
     CDS             complement(9414..10349)
                     /locus_tag="VHE33_06505"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HVT97140.1"
                     /translation="MPGGSSASTGMEGGWKEGWRAKAGMAAADQFGKGDGREPRMACQ
                     FSLIRYVPDPVKNEFVNIGVMLREAARPETVAVRFTRDWTRVRCVDADADIAMLEAME
                     AEMRQRLSADAAPLMKTIEDSFSHQIQMTEAKACLAENVAAEMDQLMQLYVEPRKLRA
                     RQTLSGRQAIAKSMRTHFEHAQVWDLMRKRIAASAYTRPGDPLKIDCGYRPNGTVRMF
                     HAVSLLGDVELAKVLAFSVPALRTGVARVENAELELTAIVEPLRNGHGDVVTGDDEVE
                     QYRFGVETMEAQQIRVLTVNDLERVAETAKRELRV"
     gene            complement(10368..11240)
                     /locus_tag="VHE33_06510"
     CDS             complement(10368..11240)
                     /locus_tag="VHE33_06510"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HVT97141.1"
                     /translation="MTVLAIQQIRKMRGGAQSHLMLGADGNAWVVKFQNNPQHLRVLP
                     NELLGTRLGALIGLTVPHCDVVEVTPWLIEQTSELEMDLGHRKERCAPGLQFGSQMVG
                     GLMPGHVADYLPEDQLMEVKNLREFAGALVLDKWTCNANGRQALFYRKGREKRYSATF
                     IDQGFCFNAAEWRFEDAPLRGVYARNAVYRDVTGWESFEPWLTHLEEVEAQRIWEIAE
                     AVPPEWYDNEGEVLERLVEKLIARRSEVRDRIVEFRESSRGPFPKWNRRAVGVREGPF
                     RGPATDDIVSGRVM"
     gene            complement(11257..12738)
                     /locus_tag="VHE33_06515"
     CDS             complement(11257..12738)
                     /locus_tag="VHE33_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97142.1"
                     /translation="MVGTREELLALGRDLDQSGDAALQLMKKILRIRNRLGRTVGLAA
                     NQAQRLYHGRASKRNIVLKARQVGVTTWIAGRFFLRTITHPGTVTVQVAHTQEAAEQI
                     FRIVHRFFEQLPEPLRSGVLKGAQKNARRIVFPGIDSEYLVETAGDPNAGRGLTLTNL
                     HCTEVARWPGDAAEILYGLMATLSPDGELALESTPNGACGCFWKEWQEADRTGTVRHF
                     FPWWVEDAYTGESVEEATITEVERQLMKEHGLTREKIGYRRQLHQSFRGLAKQEYAED
                     ANECFLVSGECAFDLDSIHKRLAEVSAPAEIRRNGTLRVWMRPEKGRNYVVAVDPAGG
                     GSEGDYTAMEVIDLETGLQCAELEEHLPPLETAAAAADLAREYNGALLAVERNNHGSG
                     VLAYLGSVHRYPRLFRDRDGEGFLTSTLSRMQMIARMGSVLAETPEAFRSERLLLQCR
                     GFIRQANGRSEAGSGEHDDCVMAMAIALAVREERLGGRKIALA"
     gene            complement(12763..>17636)
                     /locus_tag="VHE33_06520"
     CDS             complement(12763..>17636)
                     /locus_tag="VHE33_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97143.1"
                     /translation="TAVYHLNDGTVNQEYWVVPSAGNASIAAVRAQLQPSTVAVQPLS
                     KTYLDSAIASLNGSWLPVAGGTMSGPLNLNGDPSAANQAATKHYADQLAAAQLPLSGG
                     TVAGPLNAKQIEGHLYADQWQSGAGKNDGIAMSLSECATYPYACQVLAPSTYALNETP
                     PWGGYLQWIAGRTIYTGPPASVPNGTCVVDDRLGAPQVMCVPKVPPYWAQGATGWTEV
                     GPSFTVMQTNHPTDAMTGWTAPGLAVNKANYFGTRNFSAGIPNGSGQTNNTGLQVVTT
                     TFAPATTNSVANYLLANSNGDHIGLYDWDVSRGGISAGDNEGNEERFAHMEAPDVWQG
                     RVNSITPVASNDSSHSTSLCTAPCTVVNATQTQGFIGTLGDELPVVDITRGYSTGYIA
                     TIAANVVTASSSANWDAQYGVSNAHSTLTQPITNPDNRKNTFPATNYVAHVSSAAGFV
                     AGQTACVFDPSGVNWQCSNVTAVDTNANTVTLAVVQQTQWNGSTIAQGGMTGMGFRMN
                     IDCVGPGNMQGMTAVDAAPVNNICPTYPIMYNAPGNVATMVTGSRAGFATRGYGQMGS
                     GGTVTAAVSGGQVTSCSASGGSGYGSWMGLGYLLTPPQLAYTVNNGGTPPILEITGTG
                     TGGSLSSCAVINPGANLTSVSVNVTPYNPYTVFPMARAVNVWNPNTGGVDGSSIALEA
                     GGALSRFQAGDTIEQEHHYAMLLNGVLEHNFVYQPGGGVTSTWTSAIGGAFGSNHYGA
                     WMMNTNQPTLYGNYPAGATPWVAGQGMMGTPYGYTMSGSWRNGFWMKMPPYSTSGLTG
                     ALVVGCFDYVNNINVCSKWAQPYSMLLAENANTNGYGEDVLQYSIPTTTWSLTAGATS
                     FGGSGAYCTETLAGNASGGFSVSCNGKTSKLDTSGNLSLPGAVRAQGGVTGATINGEF
                     TVDGTTYSTLNAAWAAALSQANTSGQNQTVRLGPGTFAVTAPLAEPSNGACVSLIGSA
                     APTITANNGNASTVISVPSSLGGDLFSLQNTAGSQAQSCTFRNLLILANKNANHAFNL
                     QWFRGLYIDDVTINDTTQDAVYLGEASGTHQANFEMRNVTLSYSASTFTPATRPNYGL
                     NLQSTVLDSFVENLMVRNAKQAAVYNAGSGTTFELIHGFGYPYTCTTAPCNNAETNAS
                     AADASWATNYEVVDAGSGGNMYFNTYMDSPAIAAFDLKANGVQINGGHIQWPETTSFP
                     NATFAAVEATVSSNLVISNVDCLNMSNTAGIPGSPAGSSGVWISYMGANGSAPSYSSV
                     SNLAGCGQYVQQRVSARQTAFDTAGNNSSNTNYGAGQTGVTPKVFVTPLSSQAGQGGV
                     EVESFAGGQGDTFYSGFSGQASNFAVMADGAARVSGLQTGTVFATASTTLSKANHVVL
                     ANATAGGFTLTLPSCYTAMPDGLRPTGMEFTIVKTDSSGNAVQLMTTSGQTINVLGTS
                     AGTYSLSAPGGLTLACGPDSNYYSIENIAAATTNAATASALAATPSQCPAGSYATGIA
                     ANGNANCAQAWRSTWYGYFGGSFGTSANNSLGAIWSPSAAVSMTRLDIAVGTAPAGCS
                     TYPVIGVYDSTASTWLKTVTLAAGTYSYRNAVSGVSIASGHNLSMGVQTAGVGCTTNA
                     GSAQLTMEYAMNQ"
BASE COUNT         3149 a         6236 c         4771 g         3480 t
ORIGIN      
        1 tcggtcgtcg ccagaatctc aatcgccagc aaagccgcat ttgctgcgcc gggtcgccca
       61 atcgccagcg ttcccacagg cactcccttc ggcatctgaa caatggacag cagggaatcg
      121 acgccctgca gcatcgtcgc aggaatcggc acgcccagca cgggcaccag agtcttggcc
      181 gccagcatgc ccggcagatg cgccgcgccg cccgctgccg caataatgac ccgcaggcct
      241 cgtccctccg cctgcgatgc atattcaaac agcaaattgg gcgtacggtg ggctgacacg
      301 acccgcgctt cgtgcggaac cgcaaactcg cgcagaattt cgacagccgg cgcaagcgtc
      361 tcgtagtcgc tcttcgagcc catcacaacg ccgaccagag gcttctctct catcgaggtc
      421 gattctagag catcctcccg cgcggatccc aggcgcgtca ctttcgccgc agaaagtcca
      481 ctacgaactg aatgagcgtg accagcccgc cgatggcaat ggcgaaccct ctcgcactct
      541 gaaatctctc ttcgtgctgg gtcacccgtt gctcaatgac gccgatgcgc cccgaattgc
      601 catcgccgac cagcgtgacc atctggttct tcagaaccga gagatcggcc agcacctgtg
      661 cctcaaaatc tgtcatagtt cctttccatc cgtttccgtt ggcgcctgcc acgctatgga
      721 cggaagcgac gcctgttagc agcgacactg cgaaatgtca tgaatgcgtc agtggcagat
      781 tcacgaagac ggcgctcgaa aaacgcgaat agttcggcgt cgccgatccg tcgtacatgc
      841 gaatgtagta ccgctccatc gcgccctgcc tcggaatgat gaagttcggc accggcgaac
      901 gcagaaccag atccggccca gagcccgctg cgaatgacca gtcccggcgg cgcacctcga
      961 aacctccgcc ggctggcggc gtgacgcccg tgtcgatctg aattgcgctt cccgtaatcg
     1021 aggtgaccgc aagcgaaatc agatttgcca gcggtgttgc agcctccggc tgccgcacca
     1081 gccatgcgtc cgccggcagg gtggacgatg tctgaatcga aatctcttcc gcccagtcat
     1141 ttgcgaacga aatggcatac ttcgtccgca ccggcgcgcc gctcagcatc tcgatctgca
     1201 cttcgcgaac gaccaaactc gtgctgagcc cggcagaggg agatgtaatc gcgagttgat
     1261 ctccaggcca tacatcgccc tgttcgtccg cattccactc gctgtacgtc cccttccacg
     1321 cggctgcacg atcggtcgag accgccagaa tcgcggcggc ggcgttctcg cagtcggcgg
     1381 agctgcgtgg agccggccgg gtcacggttc ctgcccagca cgcggttccc ggcagcgtgc
     1441 ccccattgct ttccgcatcg atactgctcg cattcgccag acgcgcaacc gaacgccggc
     1501 tcgtccggta agacaccgca atcacatccc ccgtttgtgg ggtactcagc ggataaaact
     1561 gcagcattcc gtcgccgtta atcgtgcaat cggcgccctg cgccggcgtc ccgatcctgc
     1621 gaaccaccgg tgtaccgttt gccgatgtcg tggtaaccca caccggtcct tgcctctcca
     1681 cgctcacgtc gccgaggctg cactgcaaat accctgcaac cagcggagca tacagacacc
     1741 agggcgcggg aggggaagtg aattcgccgg agtaaagcac caccggcgtg ctcgcgatac
     1801 cgttcgtcgt gtcctggact tcgaagatga tgttcgccgc ggcgtccagc agatccgcgc
     1861 caaatctctc caggccgctg tcgggggcaa cgctatagta agcttgcaac gcccgctgca
     1921 cttcgctcga agagaatcgt aaccgcagcg tgtacaaatg gcccgcgacc gccgtaaacg
     1981 tactccccgc aatcgcgccg ttcaccagcg gagagatcgt cgtcgctgca ttcgcctgcc
     2041 cgatgagaaa ccccgcgata cagctaatca ccgtagcttg cccagcggta aaaaagccgt
     2101 tcacgatgcc ggaggtattc gctccgaact gcacgccgcc ggcctccgcc acgaatccgc
     2161 ctcccatctc cagactggag atcgcggaga ccacggtcgc accgatgctg ctgcctcctc
     2221 ctccacaggt caaaccggca gaagtcagcg cgagcgaggc gccgggatcg tcgacattcc
     2281 agatccttgt gtcgatcgtg gatccctgaa aattgtctgt cagcggcttc gacttcgacg
     2341 ccgccggcat ccagggtctt tcgctcaaat tgaacaggac gcttgtccca tcggcctgaa
     2401 agaactcggt cacataaaca cagggctcga tttcgccgca caccgttacg tcgttcgcca
     2461 gcgctctcac tctggacatc gcaagaccgc ggagtgagag tgtgccgtcc gactcgctca
     2521 gcgaatgcgt gatgctgccc acaggcagga gagtcagcgc tccattgatc agccgatacg
     2581 cactgcgaac cgacgccgcc agctcccccg cgttttcgga ccaggtgccg ctctccgtgg
     2641 gctgaaactg cgaaacgttc aggtttgatt gcgagaggtc gaagctaatc ttcccaaact
     2701 ggacagccgc gagcagcgcc tgcagagcct ctccgctgga ggctccgcaa attgttgcca
     2761 gccccggaag agcttgctgg ttcagcagaa tctcatcgct gatcgcagaa atcgcggcct
     2821 gccatacaaa cccctccgtc ccccggccga cgaattccat tgccggttcc gtgacgatgt
     2881 agcccgtgaa aagcagcgtc ccatcgtcat tcgccacgat catgcgtgca tggcgcacag
     2941 gcgtcaccag gccatgcgcg ggaaaaaggc tgacgacaca ggtcgaaggc tggttgagcc
     3001 gccgcacgat tcgaaacggc tgctcggcga cgattgcagc ggtgtagtcg attgggccgt
     3061 tgccatcatg attgtcgatt gtgagtttca atcctggcct ccgtgtcaga tcagcgttgc
     3121 gggactggga aggaaggtgc ggaagcaaac gacctgctcg ccgtcattcg cgtcggtcac
     3181 cggcagggcg cgaaatgtcg cgctcagagc aacgcgttcc agcggtggtg ccagcgtcgt
     3241 tgccgcctcc gccgcccccg ccatcgtctg cagccgcgga tagtggaaaa gaatgcgctc
     3301 accctgctca ccatcaatga cgaaaagcgc tgaccattcc tgaaagaaac tgcccccttc
     3361 gcgatcgaca aaaccggaaa tcggctgcac acgcatcgtc gctgttggcg cgcccgcgat
     3421 cagcggctgt gccagctgga gcccggccga agtgctcgac gcaacccgcg tgacgttgaa
     3481 cgacacgcgc cgaatgtagt cagcatcgtt gttcaccccc gcggcgctgg ctacataggc
     3541 agcgaagaca ccgctcccta cgaatccaat ttgtcccgca tagtcgtcat cgaccgtgat
     3601 cagcgagccc gcggcgaact gcgccgcatc ggaagccgcc attgccagga acgttggtgt
     3661 cgagcccgcc atcagattca ccggcagaag gcccttactg ccggatccgt tcgcgacact
     3721 ccctggtgca ggcacaatca gattcatgtg ctgcgatcct gcggccagag ccatcgagag
     3781 tttgctccat gctttgaatc gcaatgacac ggttgcatcg accgtctcac gcacctgaaa
     3841 ctgtgtagcg gcgggactgc ccgtggccag gcccgcgtac ttgctcgtgg atttgcgcga
     3901 aaaatcttca atccatccca gatcgatcca cggtgccggt ggcgcattcg tcgcaaaccg
     3961 gccgttcacc gatggatcga aaaccgtcgg cagtcgatgc acccgatcca ccggagcaaa
     4021 aaaggcccgt accgatcgtc ccatcggtgg cagtgccggc aacggagttg tggtttgcgt
     4081 gctcattgtt ccccctgctc ctgaaaggcg aaaaccgtga ccttcaccgt tcggcttaag
     4141 caatcgcgct gcgtcaccgc aggcccgaat tcaggttcgg accagaacac catcgtctcc
     4201 atccccaccg ccggagttgt cgtgtagttc atcttttgtg ccgagtacgg atacaaaagc
     4261 tgcagcacct cgtaatccat ctcttccagt gcccgccctc gatcaagtcc ggccctggcc
     4321 tgtgtgccgg cggtgctgta ctggatctcg cacgtcatct gcgccaggat ggcgggcagc
     4381 tgcaagtcgg cccgcatttc cgtccagttc agcgcgaagg tgtcgagaaa cggttgctgg
     4441 gcgggagctt cggcctcggt caccaggatg cccggtcgcg tcaccgctcg cagagtcatc
     4501 gtgcgtgccg gattcatact cgccagccgg ctgcgcagcg cgatatagaa cgtatctttt
     4561 gcgttttgca tcattcgtca ctcactcggt tacgttttcc cggttttaag actgtcgttg
     4621 tgctttccag gggccgccgg aatcacatcg gccactcgcc gctcgcttcc tgtaacagca
     4681 gtcgatacaa ataagcctgc ccctgatgtt ccgacgccga tatcgcctcg atcagaaacc
     4741 ctttgcccgc aatcacaacg ccgctggcca tcgcgaacag cgcctgcgcg gaactcagct
     4801 gcagctgtgt cacctgcgcg gccaccgcat cggcggaaat cagcagttca tacttcgtcc
     4861 gctggccatc ctgcagggcc tcgcgttctt tgcgaaagac caccggcgcg agcggcaggg
     4921 agagaaagcc gggcgcgttc agtccaatct gtccggcatc cgtggaatct cccatcagcg
     4981 gcggcatctg caggcaggcc gtcgttcctc cgccggtgcg cagcatcgcc gtcgccagcc
     5041 ggcgtgcgaa accactggaa ttctgtacgc ttcggtctgc catggcttat cccaccttca
     5101 ccgagaccca cggccgaagc agtgcctgca ccgtcgcgtc tatcagcgac cccgaaaaat
     5161 actgcagctg cagcgtgtcc accctcgagg acttcacgtt cagccccggt gtcgcctggg
     5221 cattcttcac gatctgtgcg caggcgcact ttaccgggct gggcagagat gccagccccg
     5281 cggtgtaggt cgcttcgatc tcgttgtagg acagacccag caggttgagc ggaagggtca
     5341 gttcaccggt ctccgccacg aaatccacgc tggctgggtc caccgccgtc catgctccgg
     5401 gcagcgagaa agcccacagg gcttcttcca gctgcggata cgccatctcc ccgcgtcgcg
     5461 gtctcgcgta tcgtgcctga atcgaaacga aggtctgcgt tgcctgggcc agcggcgcca
     5521 acggcaaata cgtcagccgc gcgctctgcg agccctccac aatacgcagc cgctcggtat
     5581 attgcgttac gctcaggctg gcgcggcggc agtgggcgtc catcagcgaa gaagccgcag
     5641 tgatccagtc gtcggtgacg tcggacgcca gcccgtacgt ctgatattcg gttggctgca
     5701 gatagcacgc catgaattct ccttactgaa gcaggttcaa aaacaacccg gccaaaaaga
     5761 gaaaggccgg ccggtgtcct gcgagcgcag gttttcgctc gccggatgcc cggttccggc
     5821 ccttcacatc gaagcccgct cgcagcgcgg tcttcgcaac acgactaccg gtcgaccagc
     5881 acttgatagt gggcgtagct ggcgcctttc acgaccggcg cgccgaactt caccaccaca
     5941 tgctgcgaag ccagagatcc cggcgttccc aattggaaga cgcgcgggtt gggatcgctc
     6001 agccagtggt actcgatcag gtcctcggtc acgatgtaag ccggcaacac cgcgctcccc
     6061 gaacctggcg tgcccgtgta agccagcgac cattccggga tcagcggcag ctcgccggcc
     6121 tgcgtggtca gcatcttcac cgtgaagccc gccgcgatct ctttggtgtt caggatcacg
     6181 ttgaactccg acttcatctc gcgatcgatc agatccagca gaaccggatt cgcgtagatg
     6241 gccgtcggcc gcaccgcata gctggtgttt gcgcacatct gcgcgattgt ggacttcagc
     6301 ccgtcggtga tgctcgcgct cgtgccgatc gtcgtggtgt tgccgcccgc tgcgatctgc
     6361 cccgcgacgc cgaaatactg catcgtggtg ggcgcgctca gcgaagtgtc gttgccgttc
     6421 cacaaagaca catcgtgcgc cagcatcacg ccctcgatcg tgtcgaccag gtccttggcc
     6481 tgcagatagg cgaactgctt ttgctgcgcg cccagctcca tgtcaaacag gttgtagttc
     6541 agctgcgcca cgatggcttt caggggcacg ctgcgctcca cacgcgtcgg cgtcaccacc
     6601 ggagcgacaa tgttgcgcgg atcgacgaag ccggcggtcg aaggattcgg aatcgccgtc
     6661 tgctcgaagt agcgtgacgg atgacccgta gccggcacct gcttaatccg ctggccgaag
     6721 agattgcggc ggcgaacgat gtcggtgatc tccgtctggt aaagaggaac ttcaaccgcg
     6781 ccgggcccga tgtagtccgc cgccgcgtga atgtcgagga aattggctgt catagcatcc
     6841 tttcgttgaa aaaatcgttg gcaacaaaaa agccggcgct cgcgccggcc gcccatctgt
     6901 tcagcgtcga tcgccttagc tcacgactcc tgcgcgcagc agctgcgcct tcaccgcaat
     6961 gcgctgttcg aggctcagcc catgcagcgc cgcatccagc gcacccgctt ccagggaatc
     7021 gagtgaagag atcccctgct tggccaggag ctgtgccgtc gatgcggaaa cggttttgcg
     7081 ccccgacttc ggcgcctctg cccgtgcctc catctgcgcg cgcagttcgg taatcgtctg
     7141 ctcggccgct tccagcttct tttgcagctc cgcctcacgg ctggtttcca cggcggcgac
     7201 gatctttcgc acttcgcccg tccgctcgct ttcctgctgc tcgatcagcg ccgctgctcg
     7261 ctcaaagagt gccgctgccg cctccaggcg cgcctccatc cgctccatgc tgtcggtcag
     7321 cgcgaccatg ttttcgcctg cctgtgcttc catgtttctt cctttcctgt tgttctcacc
     7381 ggacgggccg gaccggccgc ctccggaata cgtcggtttg tgcccgcgtg gacgcgctcg
     7441 cggagcgccg aacgggcgat gatggagcgg cagctgcgcg ccgccagctc aaatgaggtg
     7501 ttgcggtaag ccgccttctc ccgcagcagg atcgccgcgc ccgtgaacgt tgcgcgtgtc
     7561 agcgtccaga tctccgcacg catgtcctcc acgtgtgcat cggcaagctc ccatgacatt
     7621 cccatcgcgc ccggcagaca ctcgtgcatc tgccgctcga cctcgggaaa gtccttcgcg
     7681 aacagatagc ccgccacccg caggcgcgat ccgtccacgt cggcgtgcgt gatgatgccg
     7741 cacttccgcc gtgaatcgtg tccgtcccac gaaggcacgt agtcaaccgc catccccagc
     7801 agcgacggca gcgcggacag cgccgctccc cgtgtgagaa tgacgcgatg tcctcgcgct
     7861 cccgaaggcg gttttgtgct cggctcatcc accagcgtca aaactccact gaagggtgcg
     7921 cgattcggat gacccgccac ctccggcaga gtcaccgcca tggcttccag tttcatgatg
     7981 tctcccgtca tccctgcatt tcctgatctg taatcgtggt tgtgacgttt tcctcctttt
     8041 ccgttccctg cacggtggac ccgccgccct gcgacgtcgt accgtttccc gccagtccgc
     8101 tttccgcggc catcggtccc agcccgcgca tcgcccgcac ttcgttgacg gtcagcacgc
     8161 cggccgccag cagcgcggtc tgcatctgca cctcctgcat ctcgtcgctc gtctccagat
     8221 cgttgaagac gaattcgaac tccggccagc ccagccgctt ggcaaacaga tcccgcgtca
     8281 ggtgctccgc caccagccgt gccagcggca cgatggcgct gtggaaggct tcctccgtca
     8341 actccgacgc tgtcgaccga tttacatcct gctccagccc cagcagcaga ggcggcagcg
     8401 agaacgcgtt cgcgatcatc cggatcagga actcctgcca gctcaggcgc agatcggcat
     8461 ccgtgcccgc ggcaaagcgc agtacctccg gcttctgctc cgtactcagc agcggcacgc
     8521 gcccggtgcc ctcgatctcg tcctgccacc accgaatcag ccgctcatgc tgcactggcg
     8581 tcgtctcgtt cagccacagg gcgtactgca ccaccgcatt gctggccagc ttgccggcat
     8641 accgatgcgc actcagaaac gagttcaccg tctcgaacgc cacctccagc ggtcccagcc
     8701 caaacggcgt atagctgcgc ggattcatcc gcacatagat gagctggttg tctctgagcg
     8761 ggatcaccgc gtccgttccc agccgcccgg tcgcctgcgc aaaacgcacc gattccgcgc
     8821 tcccatccca cttcggatcg atgcgaatcg tcgctccatc caccgcccac agttcgaacg
     8881 gctggttctc gtctccggtc aactccatct cgatcgcgcc gaaaccgccc accagcgcat
     8941 cctcaatggt ctgttccagc agcgtccgga atgagtccga tggattcgga aattcaagcg
     9001 aacgccgcag gatccgcgct ctctccgctg accccgcttc gcgccccggc tcgcaatcgc
     9061 gcttcagccg cacctgccag tccatggccg ccacgcggtc cttaatcagg ttgatcgccc
     9121 gccgcaccac cggcgtctcc gcaaacttcc gcagattggc cggcgtcggc ttgggcagcg
     9181 cctttgccgg gaaggaatac ggggccaaaa ccgacggcaa cgccaccgtc tttcgctgcg
     9241 ccgtttcctc cctgcgtccg gcccccagca gcgccatcgc gtgtttccat tgctctctga
     9301 tcctcacgtc gtctttgctc cttcccttca tttcccaaat gaaaagggca tggcgttttg
     9361 ccatgcccct gcgagacttc cgcgccctga gctctgtgct ctgagccctg ttcttaaacc
     9421 cgcaactccc tcttcgcagt ctccgccacg cgctccagat cattcaccgt gagcacgcga
     9481 atctgttgcg cctccatcgt ctccacgccg aaccgatact gctccacctc atcatccccg
     9541 gtcactacgt ctccatgacc gttccgcagc ggctccacaa tcgccgtcag ctccagctcc
     9601 gcgttctcca cccgggccac gcccgttcgc aacgccggca cgctgaaggc cagcaccttg
     9661 gccagctcca catcgccgag cagcgacacg gcatggaaca tccgcaccgt tccgttgggc
     9721 cggtagccgc agtcgatctt cagcgggtcg cccggccgcg tgtacgccga ggccgcaatc
     9781 cgcttccgca tcagatccca tacttgtgca tgctcaaagt gcgtccgcat gcttttcgcg
     9841 atcgcctgcc ttccgctcag cgtctgtcgc gccctcagct ttcgcggctc aacgtacagc
     9901 tgcatcagct ggtccatctc cgccgccaca ttctccgcca aacatgcctt cgcctccgtc
     9961 atctggatct gatgggagaa cgaatcctcg atcgttttca tcagcggcgc ggcatctgca
    10021 ctcagacgct gccgcatctc cgcttccatc gcctccagca tggcgatatc ggcatccgca
    10081 tccacgcagc gcacccgcgt ccaatcccgg gtgaagcgca ccgccaccgt ctccggccgg
    10141 gccgcttccc gtaacatcac tccgatgttc acgaactcgt tcttcaccgg atccggcaca
    10201 taccggatca gcgagaactg gcatgccatc cgtggctcgc gcccatcacc cttgccgaac
    10261 tgatccgccg ccgccatccc cgccttcgcc ctccatccct ctttccagcc gccctccatc
    10321 cccgtactcg cgctacttcc gcccggcacg caggggactc gcgagcctca catcaccctt
    10381 ccagaaacga tatcgtccgt cgcaggcccc ctgaacggcc cctctctcac ccccaccgcc
    10441 ctccggttcc atttcggaaa cggccctcgc gacgactccc gaaattccac gatccgatcc
    10501 cgcacctccg atcgccgcgc gatcagcttt tccaccagcc gctccagcac ctctccctcg
    10561 ttgtcgtacc actccggcgg caccgcctcc gcaatctccc agatccgctg tgcctcgacc
    10621 tcctccagat gcgtcagcca gggctcaaaa ctctcccagc ccgtcacgtc gcgatacacc
    10681 gcattcctgg catagacccc gcgcagggga gcatcctcga atcgccactc cgccgcatta
    10741 aagcagaacc cctgatcgat aaacgtcgcg ctgtaccgct tctcccggcc cttgcgatag
    10801 aacagtgcct gccggccgtt ggcattgcag gtccacttat ccagtaccag cgcgcccgcg
    10861 aattccctca ggttcttcac ctccatcagc tgatcctccg gcaggtagtc cgccacatgg
    10921 ccgggcatca ggcctcccac catttgcgag ccgaattgca gtccgggcgc gcagcgctct
    10981 ttccggtgcc ccagatccat ttccagttcg ctcgtctgct cgatcagcca cggcgtcacc
    11041 tccaccacat cgcagtgcgg cacagtcaag ccgatcaacg cccccagccg cgtcccgagc
    11101 agctcgttgg gcaggacccg cagatgctgg gggttgttct gaaacttcac cacccacgcg
    11161 ttgccatccg cccccagcat caggtggctt tgcgcccctc cccgcatctt ccggatctgc
    11221 tgaatcgcca ggaccgtcac cgctcctccg cgtagctcat gctaatgcaa tcttccgccc
    11281 gccgagccgt tcctcgcgaa ccgccagcgc aatcgccatc gccatcacgc agtcatcgtg
    11341 ttcgccggag cccgcttcgc tgcgtccgtt ggcctgccgg ataaagcccc ggcactgcag
    11401 caggagccgt tcgctccgga aagcctccgg cgtttccgcc agcacgctgc ccatccgcgc
    11461 aatcatctgc atgcgcgaca aggtcgacgt caggaagccc tctccatccc gatcccggaa
    11521 caggcgcgga taccgatgca cactccccag ataggcgagc acgccggaac cgtggttgtt
    11581 gcgctctacc gccagcagcg ccccgttgta ctcccgtgcc aggtccgccg ccgccgccgc
    11641 ggtctcgagc ggaggcagat gctcctccag ttcggcacac tgcaaccccg tctccagatc
    11701 gatcacctcc atcgccgtat agtccccctc cgatccgccc cccgcaggat ccaccgcaac
    11761 cacatagttc cgtcccttct ccggacgcat ccagacacgc agcgtcccat tccgccgaat
    11821 ttccgctgga gcactcacct cagccaggcg tttgtggatc gagtcgagat cgaacgcgca
    11881 ctcgccgctg acgaggaagc attcgttggc atcctcggca tactcctgct tcgccagccc
    11941 gcgaaagctc tggtgaagct ggcgccggta cccaatcttc tcgcgcgtca ggccatgctc
    12001 tttcatcagc tgccgttcca cctccgtgat cgtcgcctcc tctacgctct ccccggtata
    12061 cgcatcctcc acccaccacg gaaaaaaatg ccggaccgtc cccgtcctgt ccgcctcctg
    12121 ccactccttc cagaagcatc cgcacgcgcc gttcggcgtc gattccagcg ccagctctcc
    12181 gtccggcgac agcgtcgcca tcagcccgta cagaatctcc gccgcgtctc ccggccagcg
    12241 cgccacttcg gtgcagtgca ggttggtcag agtcaaaccc cgcccggcgt tcgggtcgcc
    12301 cgccgtctcc accaggtatt cactgtcgat tcccggaaag acgattcggc gtgcattctt
    12361 ctgagccccc ttcagcactc cactccgcag cggctccggc agttgctcga agaagcggtg
    12421 cacgatgcga aagatctgct cggccgcctc ctgcgtatgc gccacctgca ccgtcacggt
    12481 tcccggatgt gtgatcgtgc gcaggaaaaa cctgccggca atccatgtcg tcacccccac
    12541 ctgccgtgcc ttgagcacga tattcctctt gctcgccctg ccgtgataca gccgctgtgc
    12601 ctggttcgcg gccaggccaa cggttcggcc cagccggttt ctgattcgca ggatcttctt
    12661 catgagctgc agagccgcat cgccgctctg atccagatct ctccccaggg cgagaagctc
    12721 ctccctcgtg cccaccaatt ggcaatccat ggctggtccg cttcactgat tcatcgcgta
    12781 ttccatggtc agctgcgccg atcccgcatt cgtcgtgcac cccacgccgg ccgtctgcac
    12841 tcccatggag aggttgtgcc ccgacgcaat cgaaaccccg ctcaccgcgt tccgatagct
    12901 gtacgtgccc gccgccagcg tcaccgtctt cagccatgtc gaggccgtgc tgtcataaac
    12961 gccgatcacc ggatatgtgc tgcatccggc cggcgccgtg cccaccgcaa tgtcgagccg
    13021 ggtcatgctg accgccgcgc tgggggacca gatcgctccc agagagttat tcgccgacgt
    13081 gccgaagctc cccccgaagt atccgtacca ggtcgagcgc caggcctgcg cgcagttcgc
    13141 atttccattc gccgcgatcc ccgtcgcgta ggaccctgcc ggacattgcg acggcgtggc
    13201 ggccaaagcg cttgccgtgg ccgcattcgt cgtcgccgcc gcaatatttt caatgctgta
    13261 gtagttcgaa tccggcccgc acgccagcgt caggccgccg ggcgccgaga gcgagtacgt
    13321 tccagcgctc gttcccagca cgttgatcgt ttgcccgctc gtcgtcatca gctggactgc
    13381 gttgccgctc gagtccgtct tgacgatcgt gaactccatc cccgtgggcc gcaaaccgtc
    13441 cggcatggcc gtgtagcaac tgggcagtgt cagtgtgaac ccgcctgccg tcgcattggc
    13501 caaaaccaca tgattggcct tgctcagcgt tgtcgaggca gttgcgaaca ccgtgccggt
    13561 ttgcagaccg ctgacgcgtg ccgccccatc cgccatcacc gcgaagttcg aagcctgccc
    13621 gctgaacccg ctgtaaaacg tatcgccctg ccctcccgcg aagctttcta cctccacgcc
    13681 accctggccg gcctggctgc tcagcggggt tacaaaaacc ttcggcgtga ctcccgtctg
    13741 tccggcgccg tagttggtat tcgaagaatt attccccgcc gtatcgaagg ccgtctgtcg
    13801 cgcagaaacc cgctgctgca cgtattgccc gcaacccgcc aaattgctga ccgacgagta
    13861 ggagggcgca cttccattcg cgcccatgta gctgatccag acgcctgacg acccggccgg
    13921 tgaacccgga atccccgccg tgttgctcat attcaggcaa tccacattgg aaatcaccag
    13981 attggagctg accgtcgctt ccaccgccgc aaacgtcgcg ttcggaaagc tcgtcgtctc
    14041 cggccactgg atgtggccgc cgttgatctg cactccattc gccttcagat cgaacgccgc
    14101 aatcgccggc gaatccatgt aggtgttgaa gtacatgttg ccgcccgatc ccgcgtccac
    14161 cacctcatag ttcgtcgccc acgacgcgtc cgccgcgctc gcattcgtct ccgcgttgtt
    14221 gcacggcgcc gtcgtgcacg tgtacggata tccgaacccg tggattagct cgaacgttgt
    14281 gccgctcccg gcattgtaga cagccgcctg tttcgcattg cgcaccatca ggttctctac
    14341 gaaactgtcc agcaccgtgc tctgcagatt caaaccatag ttgggacgcg tggccggcgt
    14401 aaacgtcgat gccgaataac tcagcgtgac gttgcgcatt tcgaagttag cctgatgcgt
    14461 tccgcttgcc tctccaagat agacagcatc ctgcgtcgtg tcgttgatcg taacgtcgtc
    14521 gatatacagg ccgcggaacc actgcaggtt gaaggcatgg ttcgcgtttt tattcgccag
    14581 aatcagcaga ttcctgaacg tgcagctctg cgcctggctt cctgccgtgt tctgcaggct
    14641 gaagaggtct cctcccaggc tcgagggcac gctgatcacc gtcgatgcgt tcccgttatt
    14701 cgcggtgatc gtcggagccg cgctgccgat caggctcacg cacgcaccat tgctcggctc
    14761 ggccagcggc gctgtcaccg caaaggttcc cggtcccagc cgaaccgtct gattctgccc
    14821 gcttgtattc gcctggctca gcgcggcggc ccatgccgca ttcagcgtgc tgtaggtcgt
    14881 cccatcgacg gtgaattccc cgttgatcgt ggcccctgtc actcccccct gcgcgcgcac
    14941 cgcgccgggc agcgaaagat tgccggacgt gtccagcttc gaggtcttcc cattgcagct
    15001 gactgagaat ccgccgctcg cattgccggc cagggtctct gtgcaatacg cgcccgagcc
    15061 gccaaaactc gtcgcgccgg ccgtcagcga ccaggttgtc gtcggaatgc tgtactgaag
    15121 aacgtcttcc ccgtatccgt tagtattcgc gttctcagcc agcagcatgc tatagggctg
    15181 cgcccatttc gagcagacgt tgatgttatt cacatagtca aagcatccca ccaccagcgc
    15241 ccctgtgaga ccgctcgtgc tgtaaggggg catcttcatc cagaagccgt tgcgccacga
    15301 tccgctcatc gtgtagccat acggcgtccc catcattccc tgccccgcga cccacggcgt
    15361 cgctccggcc ggataattcc cataaagtgt cggctggttg gtgttcatca tccatgcgcc
    15421 gtaatgattg gatccaaagg cgccgccgat cgccgaggtc catgtggaag tgacgccgcc
    15481 gccgggttga taaacgaagt tatgttccag caccccgttg agcagcatcg cgtaatgatg
    15541 ttcctgctcg atcgtgtctc cagcctgaaa acgcgagagg gcgccccccg cttcgagggc
    15601 gatcgaactg ccgtccacac cgccggtgtt ggggttccac acgtttaccg cgcgcgccat
    15661 tgggaatacc gtatatgggt tatagggcgt tacattcact gaaaccgagg tcagatttgc
    15721 tcctggattg atcaccgcac aactgctcag cgagccgccc gtccctgtgc cggtaatttc
    15781 caggatgggt ggtgtccctc cattgttgac cgtgtaggcc agctgcggcg gggttagcag
    15841 atatcccagt cccatccagc tcccgtaccc cgaccctccg ctggccgagc aagacgtgac
    15901 ctgccccccg ctcaccgccg ccgttaccgt cccgccgctt cccatctggc cgtagccgcg
    15961 cgtggcaaaa cccgcgcggc ttcccgtaac catcgtcgcc acatttcccg gcgcgttgta
    16021 catgatggga taagttgggc agatattgtt aaccggcgct gcatcgaccg ccgtcatacc
    16081 ctgcatattg cccggtccca cgcagtcgat attcatgcgg aaacccatgc ccgtcatccc
    16141 gccctgggca atggtcgacc cattccactg cgtctgctgc accacggcca gcgtcaccgt
    16201 attcgcattg gtatcgaccg ctgttacgtt ggaacattgc cagttcactc cgctcggatc
    16261 aaagacgcac gccgtctgcc cggcgacgaa acccgcagcg ctgctgacgt gagccacgta
    16321 gttggtcgcc gggaacgtgt ttttcctgtt atctggattt gtaatcggtt gcgtcagcgt
    16381 gctgtgcgca ttcgaaacgc cgtactgcgc atcccagttc gcgctggacg atgccgtcac
    16441 aacattggcg gcgatcgtgg cgatatatcc cgtcgagtag ccccgcgtga tgtcgacgac
    16501 cggtagttca tcgcccaaag tgccgatgaa tccctgcgtc tgcgttgcat tcactaccgt
    16561 gcacggtgct gtgcacagcg aagtcgaatg gctggaatcg ttgcttgcca ccggcgtgat
    16621 ggagttcacc ctgccctgcc agacatccgg tgcctccatg tgcgcaaaac gctcttcgtt
    16681 cccctcgttg tctcccgctg agattccccc gcgcgacaca tcccagtcat acaggccgat
    16741 atggtcgcca ttcgaattcg ccaacagata attggcaacc gagttcgtcg tggcgggtgc
    16801 gaacgtcgtc gtcaccacct gcagccccgt gttgttcgtc tgacccgaac cgttcgggat
    16861 tccggcagag aagtttctcg ttccgaaata gttcgccttg ttcaccgcca gtccgggagc
    16921 tgtccagccg gtcatcgcat ccgtggggtg attcgtctgc atcaccgtaa aagaagggcc
    16981 cacctccgtc caccctgtgg ccccttgtgc ccaatagggt ggaaccttcg gaacgcacat
    17041 cacctggggc gcccccagcc ggtcatcgac cacacacgtg ccgttcggca ccgacgccgg
    17101 cggccctgta taaatcgtcc gccccgcgat ccactgaaga tatccacccc acggcggcgt
    17161 ttcgttcagc gcataagtcg agggagccag aacctggcag gcatacggat aagtcgcaca
    17221 ctccgacagg gacatagcga tcccatcatt cttgccagct cccgattgcc actggtcggc
    17281 gtacagatga ccctcaatct gcttcgcgtt cagcggtcca gccactgtgc cccctgaaag
    17341 tggcagctga gccgcagcga gctgatccgc atagtgcttc gtcgccgcct ggttagccgc
    17401 cgatggatct ccattgagat tcagaggtcc actcatcgtc ccgcccgcca ccggcagcca
    17461 cgagccattc agcgatgcga tcgcgctgtc gagatacgtt ttcgaaagtg gctgcaccgc
    17521 aaccgtcgag ggctgcagct gggcccgcac cgctgcaatc gaagcattcc ccgccgacgg
    17581 aaccacccag tattcctggt tcaccgttcc gtcgttcaga tggtacacag ccgtgt
//
LOCUS       DASZYN010000250         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_200539, whole genome shotgun sequence.
ACCESSION   DASZYN010000250 DASZYN010000000
VERSION     DASZYN010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200539"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VHE33_06525"
     CDS             <1..751
                     /locus_tag="VHE33_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97144.1"
                     /translation="QVANCTRPYWAAWYTQNGGTCDSSATQGKSGPTPPYSVIQSDVN
                     DGAGYYEALDVNLSHHAHNGSAMLASYTWSHAIDTVDPDIPGQNPNDPRLVGEAEMGN
                     AIFDQRNRLVLSGVYAAPWGFTMGGIATMGGGLPYNYTTGVTNSGDTGATADRPVING
                     IVVGRNAGRGTAIYDLEPFVGKRITVVPERVHANLRAEAFNVLNHRNVVGFSGTYGNG
                     ATAGNGFGTPLAGITNQLPAREMQFSAQIEF"
     gene            880..>2039
                     /locus_tag="VHE33_06530"
     CDS             880..>2039
                     /locus_tag="VHE33_06530"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HVT97145.1"
                     /translation="MKRVGSAEGLVRRFVIGVVFVAGLVSAAPAVAQTTTAMPDMHWR
                     MIGPFRGGRTRAVAGVPDQPNVFYVGQVNGGVWKSTDYGRTWTPIFDHEDTQSVGAIA
                     VAPSNDNIVYVASGEGLHRPDLSIGDGIYRSDDAGKTWTHLGLRGGEQIPAIAVDPAN
                     PNRLFAAVLGHPYGPNEERGIFRSEDGGKTWKKVLYKGERIGGSDVVLDPKNPRIVYA
                     ALWEDTLGPWEDGNQYSGPGGGLFKSTDGGNTWKPLTKGLPEGLEQINVAVAPSQPQR
                     LYATMQAKGASGYATPHGLYVYRSDDGGESWAKITDDPRPEMKIGGGDLSVPVVDPKD
                     ADTVYVTSIVTCKSTDGGKTWISLRGAPGGDDYQNMWINPRNPEILALVADQG"
BASE COUNT          413 a          486 c          778 g          362 t
ORIGIN      
        1 gcaggtggcg aactgcacgc gtccctactg ggccgcgtgg tatacgcaga acgggggaac
       61 gtgcgattct tccgcgaccc agggaaagag tggaccgaca ccgccgtaca gcgtgatcca
      121 gagcgacgtg aacgacgggg cgggatatta cgaggcgctg gatgtgaacc tgagccacca
      181 cgcgcacaac ggatcggcga tgctggccag ctacacgtgg tcgcatgcga tcgatacggt
      241 cgatccggat atcccggggc agaatccgaa cgatccgcgg ctggttggcg aggcggagat
      301 ggggaatgcg atcttcgatc agcggaaccg gctggtactg agcggtgtgt atgcagcgcc
      361 gtggggattc acgatgggcg gcatcgcgac gatgggcgga gggctgccgt acaactacac
      421 gacgggcgtg acgaacagcg gagacacggg ggcgacggcg gatcggccgg tgatcaacgg
      481 gattgttgtg gggcgcaacg cgggacgcgg cacggcgatt tacgatctgg agccgtttgt
      541 cggcaagcgg atcacggtgg ttccggagcg ggtgcatgcg aatctgcgcg cagaggcctt
      601 caacgtgctg aaccatcgca atgtggtggg gttcagcggg acgtatggaa atggcgcgac
      661 ggcgggcaac ggattcggca caccgctggc ggggatcacg aatcagctgc cggcgcggga
      721 gatgcagttc tcggcgcaga ttgagttctg aggcgagcta agaagcggtc agctaaagtc
      781 cggttagcta aaaagcggtt ggcaaacacc ggtgagcgaa gaaggaagca gcggctatta
      841 caatggccgc tgttcttttt ttagaaagga agcatgctca tgaagcgggt ggggagcgcg
      901 gaaggattgg ttcgcagatt tgtcattgga gtcgtttttg ttgcggggct ggtttccgca
      961 gcgccagcgg tggcgcagac aacgacggcg atgccggata tgcactggcg catgattggg
     1021 ccgttccgcg gtgggcgaac acgcgcggtg gcgggcgtgc cggatcagcc gaatgtgttc
     1081 tacgtcggac aggtgaatgg cggggtgtgg aagtcgaccg actacgggcg cacgtggaca
     1141 ccgatcttcg atcacgagga tacgcaatcg gtgggagcga tcgcggtggc gccatcgaac
     1201 gacaacattg tgtatgtggc gagcggcgag gggttgcatc ggcctgatct ttcgatcggt
     1261 gatggaattt atcgttccga tgatgcgggg aagacgtgga ctcatctcgg actgcgcgga
     1321 ggcgagcaga ttccggcgat tgcggtcgat ccggcgaatc cgaaccggct ttttgcggcg
     1381 gtgctggggc atccctatgg gccgaacgag gagcgcggaa tttttcgctc ggaagatggc
     1441 ggcaagacgt ggaagaaggt gctgtacaag ggcgagcgga ttgggggatc ggatgtagtg
     1501 ctcgatccga agaatccgcg gattgtgtat gcggcgcttt gggaagacac gctgggtccg
     1561 tgggaggacg ggaatcaata ttcgggcccg ggcggcgggc ttttcaagtc gacggacggt
     1621 gggaatacgt ggaagccgct gacgaagggg ctgccggagg ggctggagca gatcaatgtg
     1681 gcggtggcgc cgagccagcc gcagcggctg tacgccacga tgcaggcgaa gggggccagc
     1741 ggctatgcga cgccgcacgg cctgtatgtg tatcgctccg atgacggtgg cgagagctgg
     1801 gcgaagatta ccgacgatcc acggccggag atgaagattg gcggaggcga cctgtcggtg
     1861 ccggtggtgg atccgaagga tgcagacacg gtttatgtaa caagcattgt tacgtgtaag
     1921 tcgaccgatg gcgggaagac gtggatcagc ctgaggggcg cgcctggcgg cgatgactat
     1981 cagaacatgt ggatcaatcc gcgcaatccg gagattctgg cgctggtggc cgatcaggg
//
LOCUS       DASZYN010000251         5092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_460549, whole genome shotgun sequence.
ACCESSION   DASZYN010000251 DASZYN010000000
VERSION     DASZYN010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460549"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VHE33_06535"
     CDS             complement(<1..350)
                     /locus_tag="VHE33_06535"
                     /inference="COORDINATES: protein motif:HMM:NF020701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97146.1"
                     /translation="MRFDSRYTWIEGDGPAFGAPGLQPRWTSSAKDAVGTAYAASSKI
                     WFTVSHGILNEIYCPTIDRPQIRDMQFLITDGETFFHEERRDLLHEFEYIDSDALAVR
                     VRNRDREGRYCLTR"
     gene            complement(483..1898)
                     /locus_tag="VHE33_06540"
     CDS             complement(483..1898)
                     /locus_tag="VHE33_06540"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HVT97147.1"
                     /translation="MAPFSISRRELLKRAGAAGALALGPAALAQNQAQQPKVKAGQEP
                     KPESQAAQQYPHPPGVFINRPLTQITTAADTLLDDLEGRAADFFFNEASPRTGMVRDR
                     APNTGLSLSRVSSIAATGFGLTALCIAHKRNYLRPYDCEQRVEKTLAFLLEDCPHEHG
                     FLYHFIDIESGQRMFGSELSSIDTALLLCGVLTCRQYFAGNPRIEGLATTFYRRIDWE
                     WMQNGGQTLAMGWLPDQGFLKHRWDIYSELLTMYLLAIGSPNHAIPPADWNDLQRPVV
                     QFGGLDYISGVAPLFIHQYAHAWCDFRNISDLHANYFTNSIAATRAHQLWCLTESRRF
                     PWFDQNLWGISASDSREGYRVWGGPPAMGPLDGTLVPCATGGSLVFLPAECTHVLLSM
                     REQFKEKVYTRYGFVDAFQPKANWFAPDILGIDQGITMLMAENLRTGFVWQYFMKNRE
                     IAHAMSQVQFHPDPDAMMQVL"
     gene            2208..2816
                     /locus_tag="VHE33_06545"
     CDS             2208..2816
                     /locus_tag="VHE33_06545"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HVT97148.1"
                     /translation="MAHEVPPLPYDYKALEPHIDEQTMHLHHDKHHQAYVTNLNAAIE
                     KHPDLGKHSAEDLLRNLNSVPEDVRTAVRNNGGGHVNHSMFWAIMKPNGGGEPTGAIG
                     EQIKKDFGSFEDFKKQFNETTAKQFGSGWGWVVWDGKLKIITTANQDSPLSQGFFPIL
                     GNDVWEHAYYLKYQNRRPEYLAAWWNTVNWDEINKRFAQAKK"
     gene            2964..4232
                     /locus_tag="VHE33_06550"
     CDS             2964..4232
                     /locus_tag="VHE33_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HVT97149.1"
                     /translation="MLRLRFALLLPRVRFCLLVPAALAGALLSAGGQAQRATKPASPV
                     HWVGTWATSPFDGDPWHTIPTLVDSTLREIVHTSIPGTALRVRFTNEFGKEPLRIDAA
                     NVALSAGESAIQPASMHTLTFGGQASIVIPPGAEAVSDAVPMTTPAFANLAISLFLPL
                     QQISDVSVHSGAGQTNYILAGSNAVSAATLTGAATVPSWYFVKGVDVEPVTPHAAAIV
                     AFGDSITDGAASLDNENHRWPDFLAVRLHNNPATANLAVLNEGIGGNCILIHCVAPNA
                     LARFDRDVLSQAGVRYVIVLEGINDIGALHDPNRPNYKLTAEDLEQGLSQIVARAHEH
                     GIKVFGATLTPYQGAGYFTDQGEQIREAVNQWILTSGVFDGTIDFDKTTRDPASPLTF
                     AAAYDSGDHLHPKDAGYDAMANAIDLSLFQ"
     gene            4373..>5092
                     /locus_tag="VHE33_06555"
     CDS             4373..>5092
                     /locus_tag="VHE33_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97150.1"
                     /translation="MPAGQAHFTIVQASDGKNAGSADCTVTAVTGGYQISSNGDLKMP
                     KFTYTFTNENRLDSQLNIVRDQLSGTVNGSQVTFSMNSDPSGRQFQLSIAAKGKTTNN
                     TFDRHQHTALLADLDPAAYTAMAHFALVHAPTTWIVIPKQTGLLIPAQYNAQPDVKGT
                     FHGQPVDAHHTSVVISEENGFSVELYYTSEGALLEADLPEQNFYVVRDDFKLQNRHGP
                     HRNSAPANQPQAVHLRWCSKAP"
BASE COUNT         1031 a         1387 c         1690 g          984 t
ORIGIN      
        1 tcgcgggtga ggcagtagcg gccttcgcgg tcgcggttgc gcacacggac ggcgagggcg
       61 tcggagtcga tgtactcgaa ttcgtggagc agatcacggc gctcttcgtg gaagaaggtc
      121 tcgccatcgg tgatgaggaa ctgcatgtcg cgaatctgag gacgatcgat ggtggggcaa
      181 taaatctcgt tgaggatgcc gtgagagacg gtgaaccaga tcttgctgga ggcggcgtag
      241 gcggtgccga cggcgtcttt ggcgcttgag gtccagcgtg gttggagtcc gggcgcaccg
      301 aaggcaggac catcgccttc aatccatgtg taacgcgagt cgaatctcat tgcgtcagcc
      361 attttctttg gggcgagcgg agaggagtgt taacgcacta tgaagttaga tagcaagggc
      421 gaagtttgga cgcatgttgc ggcgcagttt gcgcccgcga tgcgattgta tgggaggcag
      481 gctcacagca cctgcatcat ggcatcggga tcggggtgga actggacctg gctcatggcg
      541 tgggcaatct cgcgattttt catgaagtat tgccacacga agccggtgcg cagattttcg
      601 gccatcagca tggtaatgcc ctggtcgatg ccgagaatgt cgggcgcgaa ccagttggcc
      661 ttgggctgaa aggcgtcgac gaagccgtag cgggtgtaga ccttctcctt gaactgctcg
      721 cgcatgctga gcaggacgtg ggtgcactcg gcgggcagaa acaccagaga tccgccggtg
      781 gcgcagggga cgagggtgcc gtcgagcgga cccatggcgg gcggtccgcc ccacacgcga
      841 tagccttcac gggaatcgga agcgctgata ccccaaaggt tctgatcgaa ccacggaaag
      901 cgccggcttt ccgtaaggca ccagagctga tgcgcgcgag tggcggcaat ggagttggtg
      961 aagtagttgg cgtggagatc ggagatgttg cggaagtcgc accaggcgtg tgcgtactga
     1021 tggatgaaga gcggggccac gccgctgatg tagtcgagtc ctccgaactg caccacggga
     1081 cgctgcaggt cattccagtc ggcggggggg atggcatgat tcggcgagcc gatggccagc
     1141 aggtacatgg tgagcagctc ggagtagatg tcccagcgat gcttgaggaa gccctggtcg
     1201 gggagccagc ccatggcgag ggtctggccg ccattttgca tccattccca gtcgatgcgg
     1261 cggtagaagg tggtggcaag gccttcaatg cgcgggttgc cagcaaaata ctggcggcac
     1321 gtgagcacgc cgcagagcag gagcgcggtg tcgatggagg agagctcgct gccgaacatg
     1381 cgctggccgc tctcgatatc gatgaagtgg tagagaaagc cgtgctcgtg ggggcagtct
     1441 tcgagcagga aggcgagcgt tttttccacg cgctgctcgc agtcgtaggg gcggaggtag
     1501 ttgcgtttgt gagcgatgca gagtgcggtg agcccgaatc cggtggctgc gatgctggag
     1561 acccggctga gagagaggcc ggtgttcggt gcgcggtcgc ggaccatgcc ggtgcgcggg
     1621 ctggcctcgt tgaagaagaa gtcggccgca cggccctcga gatcgtcgag cagagtatcg
     1681 gccgcggtgg tgatctgcgt gaggggccga ttgataaaga cgccgggtgg atgaggatat
     1741 tgctgagcgg cctgggactc gggcttgggc tcctggccgg ctttcacctt cggctgctgc
     1801 gcctgattct gcgcgagggc ggcgggtccg agcgccagag ctccggcggc cccggcgcgc
     1861 ttcagcagct ctcgtcgcga tatggagaat ggagccaagt tggggaggtt cttcttttcg
     1921 aatgcagagg aatcgcgacc gatcaggttt gagaacggaa actcgggtcc tgaagggccg
     1981 gaggatcggt tgaggatcct ctacccaatt taacggaggc ccggcgcaat tcggcaactc
     2041 caccgaaagg gatgagcctt tgcggttaat tcgagcggcc aggctgcacc ctgcagccgg
     2101 ggtcgcgcat ccagtatgat cggagacgct gacctgtgcg ggtggtcccg aaccccacgc
     2161 aaccccctcg gacatttcac cactcccaca aaacaaggag gaatgatttg gcacacgaag
     2221 ttccacctct tccctacgac tacaaggctc tggagcctca catcgacgaa caaacgatgc
     2281 atctgcatca cgacaaacat caccaggcct atgtcacgaa tctgaacgcg gccatcgaga
     2341 agcatcccga tctcggcaaa cactcggctg aggacctgct gcggaatctg aacagcgtgc
     2401 cggaagatgt gcgcacggcg gttcgcaata acggcggcgg tcacgtgaac cacagcatgt
     2461 tctgggcgat catgaaaccg aacggcggcg gcgagccgac gggcgcgatc ggcgaacaga
     2521 tcaagaaaga tttcggcagc ttcgaggatt tcaagaagca attcaacgaa acgacggcga
     2581 agcagttcgg ctccggctgg ggctgggtgg tgtgggatgg caagctgaag atcattacca
     2641 ccgccaacca ggacagcccg ttgtcgcagg ggttctttcc catcctgggc aacgatgtct
     2701 gggaacacgc gtactacctg aagtaccaga accggcggcc tgagtatctc gcggcctggt
     2761 ggaatacggt gaactgggac gagatcaaca agcgcttcgc tcaggcgaag aagtaagctc
     2821 tggtttgggc tggcagcaag aggcccaaag cttaatctgg aatgcccttt ctcttgcgtt
     2881 cgcaggagga agggcatttc tgcgtaaggc agagcttatg atggaacgtt ccgatacgta
     2941 ttgccgtggg gaggactgag tcgatgcttc gtttgcgctt tgcgcttttg ctgccgcggg
     3001 ttcgcttctg cctgttggtt cctgccgcgc tggccggtgc tttattgagc gctggtggac
     3061 aggcgcagcg ggccacaaag ccggcttcgc cggtccactg ggtagggacc tgggcgacat
     3121 ccccatttga cggcgatccg tggcacacga ttcccacgct ggtcgattcc accttgcgcg
     3181 aaatcgtgca tacatcgatt ccgggcaccg ccttgcgcgt ccgcttcaca aacgaattcg
     3241 gtaaggaacc gctgcgcatc gacgcagcga atgtggcgct gagcgcggga gaatccgcga
     3301 tccagcccgc atcgatgcac acgctcacct tcggaggcca ggcctcgatc gtgatcccgc
     3361 ctggcgccga agcggtgagc gatgcggtgc cgatgaccac gcccgcattt gcgaatcttg
     3421 ccatcagctt gtttttgccg ctgcagcaga tcagcgatgt ttcggtgcat tcgggagcag
     3481 gacaaacgaa ctacattctt gccggcagca acgcggtgag tgcggcgact ttgaccggcg
     3541 cggctacggt gccgtcgtgg tacttcgtga agggcgtcga cgttgaaccg gtgaccccgc
     3601 acgcggcggc tatcgttgcg tttggcgatt cgatcacgga cggcgccgcc tcgctggaca
     3661 acgagaatca ccgctggcct gattttctgg ccgtacgtct gcacaacaat cctgcaacgg
     3721 cgaatctcgc tgtgctgaac gaaggcatcg ggggcaactg catcctgatc cattgcgttg
     3781 caccgaatgc gctggcgcgc ttcgaccgcg atgttctgtc gcaggctgga gtgcgctacg
     3841 tcattgtgct ggaaggcatc aacgacatcg gggcgctgca tgatccgaat cggccgaatt
     3901 acaagctcac cgctgaggac ctggagcagg ggttgagcca gattgtggcg cgcgcacacg
     3961 agcacggcat caaggtcttc ggcgcgacgc tgactcctta ccagggagcc ggctacttca
     4021 cggatcaggg cgagcagata cgcgaagccg tgaatcagtg gatcctgacc agcggcgtgt
     4081 tcgacggaac gatcgatttc gacaagacga cccgggatcc ggcgagtccg ctgacgtttg
     4141 ctgcggcgta tgacagcggg gatcacctgc atccgaagga cgcggggtac gatgcgatgg
     4201 cgaacgcgat cgatctgagc ctgttccagt agaaccagtc cgggaccgca tcctttgcct
     4261 ggaattcggg tctaattgcg caggaaggtc tgtcgatact atgcgttggc taaatttttg
     4321 tgcgttggtt ccggcggttt ttgcgggtgc cgggtttgcg caggcgcctc ctgtgcccgc
     4381 gggccaggcc catttcacga tcgtgcaggc gagtgacggt aagaatgccg gatcggcgga
     4441 ctgcaccgtc acggcggtca cgggcgggta tcagatcagc tcgaatggcg acctgaagat
     4501 gccgaagttc acctacacgt tcacgaatga gaatcggctc gacagccagc tgaacatcgt
     4561 tcgcgatcag ctcagcggca cggtgaacgg atcgcaggta acgttcagca tgaactcgga
     4621 tccgtcgggg cggcagttcc agctgagcat tgccgccaag ggcaaaacaa caaacaacac
     4681 ctttgaccgt catcagcaca cggctctgct ggccgatctt gatcccgcgg cgtacacagc
     4741 gatggcgcac tttgcgctgg tgcatgcgcc gacgacctgg attgtgatcc cgaaacagac
     4801 cgggctgctg attcctgcgc agtacaacgc gcaaccggac gtgaagggga cgttccacgg
     4861 ccaacccgtg gatgcacatc acacgagcgt ggttatcagc gaagaaaacg gcttcagtgt
     4921 ggagctttac tacaccagcg aaggtgcgct gcttgaggct gatttgccgg agcagaattt
     4981 ctatgtggtc cgtgacgatt tcaagctgca gaatcgtcac ggaccacata gaaattctgc
     5041 tccggcaaat cagcctcaag cagtgcacct tcgctggtgt agtaaagctc ca
//
LOCUS       DASZYN010000252         6107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_522176, whole genome shotgun sequence.
ACCESSION   DASZYN010000252 DASZYN010000000
VERSION     DASZYN010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522176"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VHE33_06560"
     CDS             complement(<1..194)
                     /locus_tag="VHE33_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97151.1"
                     /translation="MPIAPRYTDEHAQAGLDFDFPAIETWRNQFPGYEIVIDDPEFTS
                     VCPKTGLPDFGVITLRYMPR"
     gene            350..1588
                     /locus_tag="VHE33_06565"
     CDS             350..1588
                     /locus_tag="VHE33_06565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVT97152.1"
                     /translation="MQTKQARPHTVRIWTATIIGLVLIFYIVHLMTRGSLPIRVAEAT
                     VGNLTNTVSTNGKVEPQVNYEAHAPFPGVVETLNVHEGEKVSAGQLLVAMDDTEARSR
                     LATAQAALKGAQASYQAAKNGGTQEERNSLKGEIAKAQIDHDQAQQNLKTLQSLQASG
                     AASPSEVNAAQTRLAADTSTIQVLQQRLTDRYGTDYVAHAQATLADAQAGYDAALDAL
                     HHAIVHAPFAGTVYSVPVSRTEYVNQGDRLLSMADLSKLQVRGYFDEPEIGKLQTGQS
                     VTIVWDARPAETWHGHISRLPSTIITYGTRNVGEVVITIDDADDALLPDTNVRVTVTV
                     ANVSNVLMVPRDALHIERGSPFVYRKQGNSLERVKVTIGQLNLTDVQITSGLKAGDVV
                     ALGTTNAQPLSEGVPVKVVQ"
     gene            1610..2581
                     /locus_tag="VHE33_06570"
     CDS             1610..2581
                     /locus_tag="VHE33_06570"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT97153.1"
                     /translation="MQSSRALFVFSAALLGTLAAHAAHPAHRDLDLGHANSALQSLNA
                     TLAANPNDAEAHNLRCRVYYQEQDWDKAAPDCEDAVRLAPDESSFHLWLGRAYGHKAE
                     HASLMSSYQLARKVHTEFEQAVRLDPRNAAALADLGEFDVMAPIMVGGGYTRADAVVQ
                     QLDGVDPAAALTLRARIAESKKDYATAESDLKQAIRQSSDPANAWMDLASFYRRRGRL
                     DDMAAAAHSGADADPHHGPALVDGATNLICAGREPQTAIAWLREYLNSNAQSELAPSF
                     VARAQLAQLLQQQGDANGAQQQLALVHSLASGYRIPTGSAAPRAAGL"
     gene            2565..3926
                     /locus_tag="VHE33_06575"
     CDS             2565..3926
                     /locus_tag="VHE33_06575"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HVT97154.1"
                     /translation="MPLVCKLKTMDASEKIRTGAWPRHWTKFASALLLLMPLAAAAQI
                     SLSSAVDLAEKNSTAVRGAMANVQKAAAALAETKDAYIPNFVLGVSPGYSYGFPLGYP
                     SFFNASAQSLVLSWSQRDYMRAAHSALNAANLNLKDTQQQTELDVALAYVQLDSDLKE
                     LEALQQQSSYAETLVQLEQDRVSAGVDPRMTELDAELTAAQVDQRRAQLENDADAMRQ
                     KLAHLTGLPAAGLTTVSTSIPPAPSGMTGLTPQRSSSDNAGISAAYANAKAKWFTAFG
                     DDKQIYRPLAVFGAQYALFEKTPGYTEYYKNFQYNNVALGVQITFPLFDATRRAKARE
                     SSADAAHAQADADAARDLLEEQSAGLRGNLRELAAQQRVAQIQSQIAQEQLRSVETQL
                     NSGTGLPNAPGVPPTQSQKAHIQERQYYTQMLEADLALIKVQLNLMKAAGQIDAWVRS
                     SIQ"
     gene            4033..5493
                     /gene="hpnJ"
                     /locus_tag="VHE33_06580"
     CDS             4033..5493
                     /gene="hpnJ"
                     /locus_tag="VHE33_06580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HVT97155.1"
                     /translation="MPLKTLFLNPPSFENFDGGASSRWPATREIESYWYPVWLAYPTG
                     MLEGARLLDANPHHVSWPSVVEMLKDYEFLVLFTSTVGWSGDQRWAEKIKDTYPNIKI
                     AFVGPPVTTDPDKALNECHAIDFVCRREFDYSVVEYANGKPLDEILAISYRKNGQVVH
                     NPDRPQIENLDALPWVTDIYKRDLDVTKYNVPFLLHPYVALYSTRGCPAQCTFCLWPQ
                     TTSGHAWRKRSTDDVAAEMAHAKELWPNVKEFFFDDDTFNIQKARTIELCNKLKPLGI
                     TWSSTSRVTTDYETLKAMRESGCRLLIVGFESGDPQILKNIKKGATVERARDFARDCH
                     KLGLTIHGDFILGLPGETKESIRNTINFAKQLDVETIQVSVAHAFPGTEFYEYARANG
                     FITNNKMNDEGGHQLAHIEYPGLPADYVLEMVHKFYDEYFFRPKAAWRVVSKAIRNGD
                     VKRLYIESKAFLKLRSQRGKMVQEVRAQKAAASAQA"
     gene            5510..5983
                     /locus_tag="VHE33_06585"
     CDS             5510..5983
                     /locus_tag="VHE33_06585"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HVT97156.1"
                     /translation="MESVAGSQTPMTVHSMTFRRYLVLAVITLSASCGDTFLSRGMKT
                     IGPVSVAHPGELIHAIFSPWVAAGIVLLIGFFASYLTALSWADLTFVLPASSLGYVIM
                     TLLARYWLHERVSPMRWLGVVFITFGAGFVTRGPSYTDHSAHEEQREPAAEQVER"
BASE COUNT         1219 a         2022 c         1810 g         1056 t
ORIGIN      
        1 tcgcgcggca tgtagcgcag cgtgatcacg ccaaaatcgg gcaaccctgt ctttgggcac
       61 acggaggtga actccggatc gtcgatcacg atctcatatc ctggaaactg attgcgccag
      121 gtctcgatgg cggggaaatc gaaatccaga ccggcctgcg catgttcgtc ggtgtaacgg
      181 ggagcaatcg gcatacacca attttacgag gaagttgagc cggcatcaga attgcgtcct
      241 gccgctcgag ggaaccgcag tgaacacctc ggtacccagg cgaaaatcgg ttttttcgaa
      301 cccgaaagcc ccgattgcat gtcttatata ctgactcacg gtttgacgca tgcaaacaaa
      361 gcaggcaagg ccgcatacgg ttcgcatctg gactgcgacc atcattggcc tcgttctcat
      421 tttctatatc gtccacctca tgacgcgggg aagtctgccc atccgtgtcg ctgaagccac
      481 ggtcggcaat ctcaccaaca ccgtctccac caacggcaaa gtcgagccgc aggtcaatta
      541 cgaggctcat gcacccttcc caggggtggt cgagaccctg aacgtacacg agggcgagaa
      601 agtcagcgcc gggcagctgc tggtggccat ggatgacacc gaggccagat cccgcctggc
      661 caccgcccag gcggctctca aaggcgccca ggccagttac caggccgcaa agaacggcgg
      721 cacgcaggag gagcgcaact ccctgaaggg ggagatcgcg aaggcgcaga tcgaccacga
      781 ccaggcccag cagaatctga agacactgca gagcctgcag gcatccggag ccgcgtcgcc
      841 gagcgaggtc aatgcggccc agacgcggct ggccgccgac accagcacca tccaggtgct
      901 ccagcagcgc ctgaccgatc gctacggaac ggactacgtc gcccatgccc aggcgacgtt
      961 ggccgacgcg caggccggat atgacgcagc gctcgatgcc ctgcaccacg ccatcgtcca
     1021 cgcgcctttt gctgggactg tctattccgt gcctgtatcg cggaccgagt atgtcaacca
     1081 gggcgaccgc ctgctctcga tggccgatct ttcgaagctg caggtgcgtg gctacttcga
     1141 cgagccggaa atcggcaagc tgcagacggg tcagtccgtg acgatcgtct gggatgcgcg
     1201 gcctgcggaa acctggcacg gacacatcag ccggctgccc tccaccatca tcacctacgg
     1261 cacgcgcaac gtcggcgagg tggttatcac catcgacgat gccgacgatg cgctgctgcc
     1321 cgacaccaat gttcgtgtga ccgtcaccgt ggccaatgta tctaacgtac tgatggtgcc
     1381 gcgcgatgcg ctgcatatcg agcggggatc gccgttcgtg taccgcaaac agggtaattc
     1441 cctggagcgc gtcaaggtca cgatcgggca gttgaatctc acggatgtgc agatcaccag
     1501 cggcctcaag gccggggacg tggttgcgct tggcacgacc aacgcccagc ctctcagcga
     1561 aggcgttccg gtcaaagtcg ttcagtaggg atttcccggg aaaggtttca tgcagtcgag
     1621 tagagccctt tttgtttttt ccgcggccct gctggggacg ctggcagcgc acgcggccca
     1681 tcctgcgcac cgcgacctgg acctgggcca cgccaacagc gccctccagt cgctcaacgc
     1741 gacgctggcc gccaatccca acgacgcgga agcgcacaac ctccgctgcc gtgtgtacta
     1801 ccaggaacag gattgggaca aagcggctcc tgactgcgaa gacgcagtca ggctcgctcc
     1861 agacgagagc agttttcacc tttggctcgg gcgcgcctac gggcacaagg ccgaacatgc
     1921 ttcgctgatg tccagctatc agctcgcccg caaagtccat accgaattcg agcaggccgt
     1981 gcggctcgat ccgcgcaatg ccgcggcgct ggccgatctg ggcgagttcg acgtcatggc
     2041 gcccatcatg gtcggaggag gctacacgcg cgccgatgcc gtcgtgcaac agctcgatgg
     2101 cgttgaccct gcggcggccc tgaccctccg ggcccgcatc gccgaatcga agaaggacta
     2161 cgccaccgcc gaatccgatc tgaagcaagc catccggcaa tcgtcggacc cggcgaatgc
     2221 gtggatggat ctggccagct tctaccgccg gcgcggccgc ctcgacgata tggccgccgc
     2281 cgcccacagc ggcgccgacg cggatcctca tcacggaccc gcgctcgtcg acggcgccac
     2341 caacctgatc tgcgccggcc gcgaaccgca aactgcgatc gcctggctgc gcgagtatct
     2401 gaactccaac gcccagtcgg agctcgctcc gtcctttgtg gcgcgcgccc agctggcgca
     2461 gcttctgcaa caacagggcg acgccaacgg cgctcaacag cagctcgcgc tggtgcattc
     2521 gctggcctcc ggttacagaa tccccacagg aagcgccgct ccgcgtgccg ctggtctgta
     2581 aactcaaaac gatggacgcg tcggagaaga tccggacagg ggcctggcct cggcattgga
     2641 cgaaattcgc cagtgccctg ctcctgctga tgcctctggc cgcagccgcc cagatttctc
     2701 tctccagcgc cgtggatctg gccgagaaga acagcaccgc cgtccgcggc gccatggcca
     2761 atgtgcagaa ggccgccgcc gccctcgcgg aaacaaaaga cgcctatatc ccgaacttcg
     2821 tcctcggtgt cagccccgga tattcctacg gctttcctct ggggtatccg tcgttcttca
     2881 atgcgagcgc ccagtctctg gttctttcct ggtcgcagag ggattacatg cgcgcggcgc
     2941 acagcgctct gaatgcggcc aacctcaatt tgaaggacac gcagcagcag acggaactgg
     3001 acgtggcgct cgcctacgtg cagctcgact ctgacctgaa ggagctggag gccctccagc
     3061 agcagagcag ctatgcggag actctggtcc agctggaaca ggatcgagtg agcgccggcg
     3121 tcgatccgcg catgaccgag ctggacgccg agctcaccgc tgctcaggtc gaccagcggc
     3181 gcgctcaact ggaaaacgac gccgatgcca tgcgccagaa gctggcgcac ctcacaggat
     3241 tgcccgccgc cggactcacc accgtcagca ccagcatccc gccggcgcct tccggcatga
     3301 cagggctcac ccctcagcgg tcttcgtccg acaacgccgg catatcggcg gcgtacgcca
     3361 atgccaaagc gaaatggttt accgccttcg gcgatgacaa gcaaatttac cgtcccctcg
     3421 ccgtcttcgg cgcacagtac gccttgttcg aaaagacgcc gggctacacc gagtactaca
     3481 agaacttcca gtacaacaac gtcgcactgg gggtacaaat caccttccct ctcttcgacg
     3541 caacgcgccg cgccaaagcc cgcgaatcca gtgccgacgc ggcccacgcc caggcggatg
     3601 ccgatgcggc ccgcgacctt ctcgaggaac aaagcgccgg ccttcgtggc aatctccgcg
     3661 aactcgccgc gcagcaacgc gtcgcgcaga tccagagcca gatcgcccag gagcagctca
     3721 ggagtgtcga aacccagctc aacagcggga ccggactgcc gaatgctccc ggcgttccgc
     3781 ctacgcagtc gcaaaaagcc cacatccagg aacggcagta ttacacccaa atgctcgagg
     3841 ccgacctggc tctgatcaag gttcagctga atctcatgaa agccgcaggc cagatcgatg
     3901 catgggtgcg ctcatcgatc caataactta ttgcgccgcg tgaggcgcta aacttctgat
     3961 tcctcaggca tctaaatcgg tccccggcca cccctgctgc ggttgccgaa tggtaaactt
     4021 ggaggagtgc ctatgccgct taaaacgctg ttcctgaacc ctccctcctt cgagaacttc
     4081 gatggcggcg ccagctcgcg ctggcccgcg acccgcgaaa tcgagtccta ctggtacccg
     4141 gtttggctgg cctatccaac gggcatgctc gaaggcgcgc gcctgctcga cgccaacccg
     4201 caccatgttt cctggccgag cgtcgtcgag atgctgaagg actatgagtt cctcgtcctg
     4261 ttcaccagca ccgtgggctg gagcggcgac cagcgctggg cggagaagat caaggacacc
     4321 tatccgaaca tcaaaatcgc gttcgttgga ccgccggtca ccacggatcc cgacaaggcc
     4381 ctgaacgagt gccatgccat cgactttgtc tgccgccgcg agttcgatta ctccgtggtg
     4441 gagtacgcca acggcaagcc gctcgatgag atcctggcga tcagctaccg gaagaacggc
     4501 caggtcgtgc acaatcccga ccgcccgcag atcgagaacc tcgacgcgct gccctgggtc
     4561 acggatatct acaagcgcga cctcgacgtc acgaagtaca acgtgccgtt cctgctccac
     4621 ccgtatgtcg cgctttattc cacgcgcgga tgcccggcac agtgcacctt ctgcctgtgg
     4681 ccgcagacca cgagcggaca cgcgtggcgc aagcgctcga ccgacgacgt cgccgcggag
     4741 atggcccacg cgaaagaact ctggccgaac gtgaaggagt tcttcttcga cgacgacacc
     4801 ttcaacatcc agaaggcgcg gaccatcgag ctttgcaata agctcaagcc gctgggcatc
     4861 acgtggtcgt cgacctcgcg cgtgaccacg gattacgaga cgctgaaggc gatgcgtgag
     4921 tccggctgca ggctgctgat cgtgggcttc gagtccggcg atccgcaaat cctcaagaac
     4981 atcaagaagg gcgccacagt ggaacgtgca cgggactttg cccgcgactg ccacaagctc
     5041 ggcctgacga ttcacggcga cttcatcctc ggtctgcctg gcgagacgaa ggaatcgatt
     5101 cgcaacacca tcaacttcgc caaacagctc gacgtcgaga ccattcaggt ttcggtcgcg
     5161 cacgcattcc ccggcaccga gttctacgag tacgcgcgcg ccaacggctt catcaccaac
     5221 aacaagatga acgacgaggg cggacaccag ctcgcgcaca tcgagtaccc gggtctgccc
     5281 gccgactacg tgctggagat ggtgcacaag ttctacgacg agtacttctt ccgtcccaag
     5341 gcggcgtggc gtgtggtcag caaggccatt cggaacggcg acgtgaagcg gctctacatc
     5401 gaatcgaagg cattcctgaa gctgcgctca cagcgcggca aaatggtgca ggaagtgcgc
     5461 gcccagaagg ccgcagccag cgctcaggcg tagaggtgcg gccggcagcg tggagtccgt
     5521 ggccggcagc cagacgccta tgaccgtcca ctcgatgaca ttccggcggt acctggtgct
     5581 ggccgtcatc acgctcagtg catcgtgtgg cgatacattt ctgtcgcgcg ggatgaagac
     5641 gatcgggccg gtctcggtgg cgcatcccgg cgagctgatc cacgctattt tttcgccgtg
     5701 ggtcgctgcg ggcattgtgc tgctcatcgg cttcttcgcg tcgtatctga ccgcgctctc
     5761 ctgggctgat ctcacctttg tcctgccggc gagttcgctc ggctacgtga tcatgacgct
     5821 gttggcgcgg tactggctgc acgagcgcgt ctccccgatg cgctggctgg gagtggtgtt
     5881 catcaccttc ggcgcgggat tcgtgacgcg cgggccgtcg tataccgatc acagcgcgca
     5941 cgaagagcag cgagagcctg cggctgagca ggtggagcgg tgaacagccc tcacgccatt
     6001 ctcgcgacct gcgccatgat cgcggctgtt gttatcacgg cgactgccgg cgatattctt
     6061 accgcgtcgg cgatgcgcca ggttggcgat ctcgatgcga tccgcgc
//
LOCUS       DASZYN010000253         4303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_74349, whole genome shotgun sequence.
ACCESSION   DASZYN010000253 DASZYN010000000
VERSION     DASZYN010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74349"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(375..2552)
                     /locus_tag="VHE33_06590"
     CDS             complement(375..2552)
                     /locus_tag="VHE33_06590"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 protein"
                     /protein_id="HVT97157.1"
                     /translation="MKVAHARMALLLALGFFFPARNASAEVHATLVTADTTIEVAGGP
                     EAPRLVSLAGHNARPWRNRSVDTLPPWVEREGKRLPLHWRLVPGAAAVDSHHVAIVYQ
                     TTVPRLRLTWEWSARAPFGPLEHTVTIRNRSGVELWLPIPDSLHVDWQVPDSAELRNL
                     YIDKGAGAPTDQGTHLDPVPMGYSWTGWSSTFAHPVPGHPREIIPFEAIFAPQPDAAG
                     WFAGIEFSGRTRIALARTPAGLTSDLGLDPDPGPALTRLIPGGDFSTPRIFLGSFTGG
                     PDGAGNQLRPWVRRVLGSPRTWDDPEYPITVNNSWGVGMSISDATARRMIADASSLGF
                     EMFHLDAGWFRGVGDWYPNPQKFPNGLAPIAEDAHHHGMRFGLWVDWAQAGIDTEPGA
                     LNLNDPKVRNWLVADIGPGWKPQDFKGQTIDLGVPAAHDYAAREVDRIVTTNHLDMLE
                     HDGYLVAQGCTRDDHPHAPPDRQHMQVEHYDGEDIVLSSNSTDVSYHAVEAYYDIYAH
                     LRKTHPGLLFEICNDGGRMVDFGAAAHGDYFSITDSYDPLSNRRAFYDASYVLPPPML
                     ETYVDKWPTPHLGNFVYELRSGMMGWFSVMQDTNAWTADQHEAARRELALYKSRLRPL
                     IRDARLFHVAPRPDGVHWDGIEYWDPARRAGVVFAFHGSSASEPTHRYVLDDLNSGKS
                     YRIHFEDGSSQDTIASGRQLMEKGLTVDLPQPLTSELIFLSEH"
     gene            complement(2570..>4303)
                     /locus_tag="VHE33_06595"
     CDS             complement(2570..>4303)
                     /locus_tag="VHE33_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97158.1"
                     /translation="AFAWSKIALDQAWVCNDGIGCGEVGGYGPSRPERRPQYAWFFAG
                     DGLVATEGLLAEGDIEHARQELAFIFRYQNPENGMIWHEISQSAKYLDWSGKYPYMYV
                     HVDITFQFLATLIDYYQATGDVDFLRDHWSGITKAWTYCRSLIDPSTSLPQIPAGKEG
                     GNEQDRMREDAGLSAAWVAASDAYGQLALAVGDTGQAEAAAGAHDAAQKALAGRYWDP
                     QKQFWIAGFAEDGHAMTDERSHSGLLGYGFLQPAQEDAALERLASAHFETDWGTRSIS
                     DASPSYKPDSYGAGSVFELNTAETAEAFWRDHRPAIAQGVWTSMLPAVQLDSLGHLPE
                     VLSGDRFQPQVESVPEQTWSSAGLVHSLMQGVFGLVADAPEHRLTLAPHLDPRWNHVG
                     LESVPVGAARVDIAIDQQPGEIHADLAARDAPVHLRFAPQIPLGATNVSASLDGHALP
                     VAIDRHEEDEHARIEIDLPPGSRHCRILFDGGVRVRVPAAHPVPGASSRALKLVSLHL
                     VGHRLALDADVAAADSASLELETPWKIQSVQGAQITRHQGAWYTLAFPASPAQPGGYT
                     RKTATVEFSPR"
BASE COUNT          810 a         1223 c         1496 g          774 t
ORIGIN      
        1 tcacgtctgg tttccggcag aggcacatct ggtcaacggc atcgttgtgg tctcgacttc
       61 gcttgttcag caacccatcg cggtgcgata tcactggacg aacataaaaa ccgcggcgct
      121 gttcaacggt gcaaaccttc ctgctggacc tttccaggcc cacgaatagc tgcgcccgtg
      181 ccttctatcg aaactcgaca tgccgggttc gggatcgtgt tagaggaagc gcggaaagcc
      241 gcgttgaagt tcgcgcgagg aagaaccagg tcgcttccgg aaagcccggg gaattgtgga
      301 gcctggttga tcgcgatcct gaggaacgga gctatcccgt atcttctggt tgcgaatcct
      361 gttcttcgat ggcctcagtg ttccgacagg aagatgagtt ctgacgtgag gggttgcggc
      421 aggtccaccg taagaccctt ttccatcagt tgccgaccgc tggcgatcgt gtcctgggag
      481 cttccgtcct cgaaatggat tcggtaggac ttcccggagt tcaggtcatc gaggacatat
      541 cgatgggtcg gctcgctggc cgatgaaccg tggaaggcga agacaacgcc cgcgcgacgg
      601 gcggggtccc agtattcgat gccgtcccag tggacgccat cgggacgcgg cgcgacgtgg
      661 aacagccgcg catcgcgaat cagggggcgc agccgcgatt tgtagagagc gagctccctg
      721 cgcgcagcct catgctgatc ggcggtccag gcgttcgtat cctgcatgac gctgaaccag
      781 cccatcatcc cgctgcgaag ctcgtagaca aagttgccga gatggggagt cggccacttg
      841 tcgacgtacg tctccagcat gggcggggga agaacgtaac tggcgtcata gaaggcgcgg
      901 cgattggaga gagggtcgta actgtcggta atggagaaat agtctccgtg ggcggcggcg
      961 ccgaagtcga ccatgcggcc gccatcgttg caaatctcga agagcaggcc gggatgggtt
     1021 ttgcgcaggt gcgcgtagat gtcgtaatag gcctcgaccg cgtgataact gacatcggtg
     1081 gagttggagc tgaggacgat gtcttctccg tcgtagtgct cgacctgcat gtgctgacga
     1141 tcgggcgggg catggggatg gtcgtcgcgt gtgcagccct gggcgacgag gtagccatcg
     1201 tgctcgagca tgtccagatg gttggtggtc acgatgcgat cgacttcgcg cgcagcgtaa
     1261 tcatgggcgg cgggaacgcc aagatcgatg gtctggccct taaagtcctg gggcttccag
     1321 ccgggaccga tatcggcgac cagccagttg cggactttgg ggtcgttcag attgagcgcg
     1381 ccgggctcgg tgtcgatgcc cgcctgggcc caatcgaccc acaggccaaa gcgcatcccg
     1441 tgatggtgag cgtcttcggc gatgggcgcc agaccgttgg gaaatttctg cggattgggg
     1501 taccagtcgc cgacgccgcg gaaccagccg gcatcgaggt gaaacatttc gaagcccaaa
     1561 gaggatgcat cggcgatcat gcggcgcgcg gttgcatcgc tgatggacat accgacgccc
     1621 caactgttgt tgacggtgat cggatactcg ggatcgtccc aggtacgagg agagccgagc
     1681 acgcggcgca cccacggacg cagttgattg cctgcgccat cgggaccgcc ggtgaaggag
     1741 ccaagaaaga tgcgcggggt gctgaagtct ccgccaggga tcaggcgtgt gagggcgggg
     1801 ccaggatcgg ggtcgagccc gaggtcgctg gtgaggccgg ctggcgtgcg cgcgagtgcg
     1861 atgcgtgttc gaccgctgaa ctcgatgccg gcgaaccatc cggcggcgtc gggctgcggc
     1921 gcgaagatgg cttcgaacgg aatgatctct cgcgggtgtc cgggaacggg atgagcgaaa
     1981 gtggaggacc agccggtcca gctatacccc atgggcacgg gatcgagatg agttccctgg
     2041 tcggtgggtg cgcctgcgcc tttgtctata tacagattgc gaagttccgc ggaatcgggg
     2101 acctgccaat cgacgtggag gctgtcgggg atggggagcc agagctcgac tccgcttcgg
     2161 ttccggatcg tgaccgtgtg ttccagcggg ccaaagggcg cgcgcgcaga ccattcccag
     2221 gtgaggcgaa gacgcgggac ggttgtttgg tagacgatcg ccacgtgatg ggaatccacg
     2281 gcggcggcgc cagggacgag gcgccagtgc aggggcaggc gtttgccctc gcgttccacc
     2341 catggcggca gagtatcaac ggagcgattt cgccaggggc gagcgttgtg gccggcgagg
     2401 gagacgagac gcggcgcctc ggggccgcct gcgacttcga tggttgtgtc ggcggtgaca
     2461 agcgtggcat ggacttcggc cgacgcattt cgagcaggga agaaaaaacc aagggcaagg
     2521 agcagagcca tccgagcatg ggctactttc atgatccctc cacgcggtgt cagcggggac
     2581 tgaattccac ggtcgcggtt ttgcgggtgt atccgcctgg ctgagcgggc gatgcgggaa
     2641 aggcaagcgt gtaccacgca ccctggtggc gggtgatctg cgctccctgc acggactgaa
     2701 tcttccaggg ggtttctaac tcaagcgaag cgctatccgc ggcggcgacg tcggcatcga
     2761 gcgcgagacg gtgtccgacg agatgcagag agacgagctt cagcgcccgg ctggatgcgc
     2821 caggcacagg atgagccgcg ggaacgcgaa cacgcactcc gccgtcgaac aggatgcggc
     2881 aatggcgcga gccgggcggc aggtcgatct cgatgcgcgc atgctcgtcc tcctcgtgcc
     2941 ggtcgattgc gaccggaaga gcatggccgt caagactggc gctgacgttg gtggcgccga
     3001 gaggaatctg cggggcgaag cgaagatgca cgggggcgtc gcgtgccgct aaatccgcat
     3061 ggatttcgcc gggctgctga tcgattgcga tatccacgcg ggcggcgcct accggcacgc
     3121 tttcgaggcc aacgtggttc cagcgtggat ccagatgggg cgcgagggtc agccgatgtt
     3181 cgggagcatc ggcaacgagc ccgaacacgc cctgcataag ggaatgcacc aggccggcgg
     3241 aagaccaggt ttgctccggc acggactcca cctgaggctg gaaccgatcg ccggagagca
     3301 cttcagggag atggccgaga gaatcgagtt gaactgcggg gagcatggag gtccaaactc
     3361 cctgggcgat ggcgggacga tggtcgcgcc agaacgcctc cgcggtctct gcggtgttca
     3421 gctcgaaaac gctgcctgcg ccgtatgagt ccggcttata actgggcgac gcatcggaga
     3481 tgctgcgggt accccagtcc gtctcgaagt gcgcggaggc cagacgctcg agcgccgcgt
     3541 cttcctgtgc gggctggaga aagccgtagc cgagcaaacc ggaatggctg cgctcatcgg
     3601 tcatggcgtg gccatcttcg gcaaaacccg cgatccagaa ttgtttctgc ggatcccagt
     3661 agcggcccgc aagagccttt tgcgccgcgt catgtgcgcc ggccgctgct tcagcctggc
     3721 ccgtgtcgcc caccgccagt gcaagctggc catacgcgtc cgacgccgct acccaggccg
     3781 cggacaggcc agcgtcttcg cgcatgcggt cctgctcgtt gccgccttcc ttgccggcgg
     3841 gaatctgcgg cagcgaggtt gatggatcga tcagggagcg gcaataggtc caggctttcg
     3901 tgatgccact ccagtgatcg cgaaggaaat ccacatcgcc ggtggcctgg tagtaatcga
     3961 tcagcgtcgc gagaaactga aacgtgatat cgacgtggac gtacatgtag ggatatttcc
     4021 cggaccagtc gaggtacttc gcactctgcg aaatctcatg ccagatcatt ccgttctcgg
     4081 gattctgata gcggaagatg aaggccaatt cctgtcgggc gtgttcgatg tcgccttctg
     4141 cgagcaaccc ctcggtagcg acgaggccat cgccggcaaa gaaccaggcg tactgggggc
     4201 ggcgctcggg acgggagggg ccgtatccgc cgacttcacc gcaaccgatg ccgtcgttgc
     4261 aaacccacgc ctgatccagc gcgatcttcg accaggcgaa cgc
//
LOCUS       DASZYN010000254         1940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_329576, whole genome shotgun sequence.
ACCESSION   DASZYN010000254 DASZYN010000000
VERSION     DASZYN010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329576"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="VHE33_06600"
     CDS             complement(<1..1349)
                     /locus_tag="VHE33_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT97159.1"
                     /translation="MPTYTMLRRGLLLVLLLSATGWARENGSGWLEVRSPHFTVITDA
                     GEGRGRHVAGQFERMRWVFQSSLPASANLDPANPIVVIAVKDRKGMEALEPAPYLAKG
                     QISLAGLFSQSTDKNYILVRLDGEGEHPWSTVYHEYTHLMEANSAEWMPLWLNEGLAE
                     FFQNTEIRDKEVLLGEPSMSALLLLQQNRLIPLPVLFKVDATSPYYHEEGKGGMFYAE
                     AWALTHSLEVADFRDHGNRIGKYLALTSRGTDAVAAAEQAFGDLTKLQQELETYVREG
                     RYSFFHRSTEQFRIDERSFAVAELGVADADAIRADFLANMRRPEDARAMAEAALQADP
                     NNARAHEAMGRIEYVAGNREEARKWYGEAAALDPENYVAQYYTGSLAMMAGESNAEAE
                     EHLRAAIRGNPRFAPAYDALAAVLLDRQGKLDQAHMMELQAVALDPGNLHYRLNTAQ"
     gene            1520..1762
                     /locus_tag="VHE33_06605"
     CDS             1520..1762
                     /locus_tag="VHE33_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97160.1"
                     /translation="MAAQEGLHRIRLVGKAILVLGLLLDAVALAGGLTAALFHQSAWT
                     AGFAPFGIALSMAGAAILLTAWIIEGFTLPPRPPHV"
BASE COUNT          332 a          755 c          493 g          360 t
ORIGIN      
        1 atctgcgccg tgttcagccg gtaatgcagg ttccccggat ccagcgccac cgcctgcaac
       61 tccatcatgt gcgcctggtc gagtttgccc tgccgatcga gtaacaccgc cgccagcgcg
      121 tcataagccg gcgcaaaccg cggattcccc cggatcgccg cacgcaaatg ctcctccgcc
      181 tccgcgttgc tctctcccgc catcatcgcc agcgaccccg tgtaatactg cgccacgtaa
      241 ttctccggat cgagcgccgc cgcctcgcca taccacttcc tcgcctcctc gcgattcccg
      301 gcaacatact cgatccgccc catcgcctca tgcgccctcg cgttgttcgg gtccgcctgc
      361 agcgccgcct ccgccatggc gcgcgcatcc tcgggtcgcc gcatgttcgc aagaaaatcc
      421 gcccggatcg catccgcatc cgccacgccc agctccgcca ccgcaaacga ccgctcgtcg
      481 atccggaact gctccgtcga ccgatgaaag aagctgtacc gcccctcgcg cacatacgtc
      541 tcgagctcct gctgcagctt cgtcagatcg ccgaaggcct gctcggccgc cgccaccgca
      601 tccgttcccc ggctggtcag cgccagatac ttcccgatcc gattcccgtg atcacgaaag
      661 tccgccacct cgagagaatg cgtcagcgcc cacgcctcgg cgtagaacat gccccccttc
      721 ccctcttcgt ggtagtacgg cgacgtcgcg tccaccttga acagcaccgg cagcgggatg
      781 agacggttct gctgcagcaa cagcaaagcg ctcatgctcg gctcgcccag cagcacctct
      841 ttgtcacgga tttccgtgtt ctgaaagaat tccgccagtc cctcgttcag ccacagcggc
      901 atccactccg ccgagttcgc ctccatcagg tgcgtgtact cgtggtacac agtcgaccag
      961 ggatgctctc cctccccgtc cagccgcacc aggatgtagt tcttatcggt cgactgcgag
     1021 aacagtccgg cgaggctgat ttggcccttc gccagatacg gcgcaggctc cagcgcctcc
     1081 atgcccttcc ggtccttcac cgcgatcaca acaatgggat tggccggatc cagattcgcc
     1141 gacgccggca ggctgctctg aaacacccac cgcatgcgct cgaactgccc cgccacatgc
     1201 cggccccgcc cctcgcccgc atcggtgatt accgtaaagt gcgggctgcg cacctccagc
     1261 cacccggacc cgttttcgcg cgcccagccg gtggccgaaa gcaataaaac aagcagcagc
     1321 cctcttcgca gcatcgtgta cgtgggcatt ctcccacgct tcagtctcca ctggctctcc
     1381 tgaacaggca cgattaactg ggtgccccat atctgcccgc ctttggcaga tgtgggaacg
     1441 ccagatgatc cccgaaccgg tctttcactc gctgcaaaac cccgcgttca tcagcgctgc
     1501 ggggtggtag agtggcgcca tggccgctca ggaagggctc caccgtattc gcctcgtggg
     1561 caaagccatt ctcgtcctcg gccttctgct ggatgctgta gccctggctg gcggcctgac
     1621 cgcggccctc ttccatcagt cggcctggac cgccggcttc gcccccttcg gcatcgcgct
     1681 ctccatggcc ggcgccgcga tcctcctcac cgcctggatc atcgaaggct tcacgttgcc
     1741 accacgcccg ccgcacgtct gatcgtccga taaactaaca acagaaacgt tccgatgacc
     1801 ctccttaaag ccgtgcgcgg cacgcgcgat cttctgccgc ccgaaaccga actctgggac
     1861 cgcgtcgaag ccaccacccg ccgcgtcttc gctcgctaca acttcggcga gattcgcacc
     1921 cccctcttcg aagacaccgc
//
LOCUS       DASZYN010000255        13539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_201005, whole genome shotgun sequence.
ACCESSION   DASZYN010000255 DASZYN010000000
VERSION     DASZYN010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 13539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13539
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201005"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VHE33_06610"
     CDS             <1..408
                     /locus_tag="VHE33_06610"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HVT97161.1"
                     /translation="WQGGLLTRAIILIRILVGWVFVSEGIQKFLFPALGVGRFAKIGF
                     PHPGILAPIVGIVEIACGLAVILGLWTRLASIPLLIVIGTAIVTTKIPELHRAGEGFW
                     FMLHDARTDFSMVLGLVFLLVVGGGTLAMTRSR"
     gene            774..2090
                     /gene="ligD"
                     /locus_tag="VHE33_06615"
     CDS             774..2090
                     /gene="ligD"
                     /locus_tag="VHE33_06615"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HVT97162.1"
                     /translation="MKKSEAAEVLSIGGHEIAITHPDKLYFSKQVRLTKIELVRYYLS
                     VAEGALAGVRDRPLVLKRFVNGAEAEAFYQKRAPTPRPDWLRTVTLSFPSGRTAEEVV
                     LDSVAGLVWVGNLGCIELHPHAVRASDLDHPDELRIDLDPGPGVGWDDVRKVSLEVKA
                     FLEEVGLRGWPKTSGSRGMHVNIRIEPRWSFEQVRQAALALSREIERRVPQLATSKWW
                     KEERHGVFLDYNQNAKDRTTASAYSVRPVPDARVSTPLRWEEVTECDPADFTVFTVPE
                     RFAKIGDPHAEMDASAGSLEKLLEFAARDEASGLGDAPWPPHFRKMAGEGARVAPSRQ
                     KKASPSVAKKPREKMPLVVVANSPDKDAALAGLERWKAAHADAAKHLAVDDVLVDSMR
                     GRSSTWTRIRVNLRHVPEDLRPAQETPDPDEDPTREWRSRTRKKSE"
     gene            complement(2244..3359)
                     /locus_tag="VHE33_06620"
     CDS             complement(2244..3359)
                     /locus_tag="VHE33_06620"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HVT97163.1"
                     /translation="MNLPVNPPVLPMLSKRVAKLPTGAEWIYEPKWDGFRALIFRDGD
                     EVLIQSRDSKSLNRYFPELIEPILEQLPRACVLDGEVVIARNGVLDFDALQLRIHPAA
                     SRVKLLSQQTPASIVFFDLLAEGAHDWTGKPFHERRARLESILSGAKPPLHITPATDD
                     PAQAGDWFGRFEGAGLDGVMAKSKQGLYEPDKRTMFKVKHERDCDCVVAGFRWYRKGT
                     QPAIGSLLLGLYDDSGALQHVGVCGSFPMQKRIELVEFLEPYRKNALKDHPWADWTGS
                     AENSEGDSENSKEANVASRRPGGQSRWSQGKDLSWEPLRLELVAEVAYEHMQSGRFRH
                     MAQFRRWRKDKEPKDCTYEQLEVVPPEELKAIFPTNR"
     gene            complement(3411..5192)
                     /locus_tag="VHE33_06625"
     CDS             complement(3411..5192)
                     /locus_tag="VHE33_06625"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HVT97164.1"
                     /translation="MTTLRTAKRFVFPLLGIWVLLYGSFSLFKPPLLDGSDALSAEIA
                     REMLTGGHWLTPFANGIVYPHHPPLLYWTVAVSFGIFNVSDWAARLPVAIAILGLCIA
                     TFSLGRRIFHSPAAAFYAVLTLLTSYGVFLFGHLLIRDVFLCLWTTLALNFFWRSLTQ
                     QKRLLESALGFATCCALGVLTQGLSGLLLPLIIAVLYLAFTHNLGHLRRWHPIPAILT
                     FLVLYLPWHFAARHALRQQDLMNLLPRFTGGRVPLIVFWPLLLLWIIPWCAYSLRALR
                     IGASPEPDRRRQARCFSLIWILTVLLFFSFTARQEFNLLTALPPMALLAGGWLAEDEA
                     QLHHEGRIAAWILFFFGLAASALIAYYLIVAPVPAPGVDIATLIRSHPGHHAVFFAYF
                     LDLTRNTMGAVRGPLWITLIATLVGVSVSLFYRLRDNARMANCFLAGMIVAILIAAHL
                     ALNTFSPVLSSEILAEAIHPEVQPADLVVVNGHIDDASSFVFYLERNVLLLNHVAPNE
                     SVPASCSGNVPVTTKTTVLSYVGPPPQPFFINHRGLCGIWQSPARVWLWTPLEDVPTL
                     PGNVYVIGRSGGKEVLSNQPNTGGASF"
     gene            complement(5274..6560)
                     /locus_tag="VHE33_06630"
     CDS             complement(5274..6560)
                     /locus_tag="VHE33_06630"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HVT97165.1"
                     /translation="MSALASPKFEAPPQPGAHAPANLTPHRFLGRQPILDAERRLFGY
                     ELLYREGKTDHFSGDPEHATREVVDHWLMLIPDHRQGAAFVNCTRNAIVDGLITLLPP
                     ESTVLEILEDIDPDPALINACLALKSKGYRFALDGFLPRPSRAPFLALADFIKVDFMT
                     ADFLVRREIYALASGTPAQLVAEKIESDAQLRIAIAEGCSLFQGYFFSQPILVQSHAI
                     PRNHTVYLSLLAQLRQGSTDLRKLEKIIMGDAALCFRVLRLANSALQHHPGAITTIAE
                     ALLMIGEDALRRLITVAVTGALGAQQSSALIPMILTRARFCELLAPSFREDPAELYLL
                     GMLSLLDALLQMPFSRILQSIPISNEMKLALAGDASPAGLTLALVRGLESCDWGVCEE
                     IQHKLGLAEGVIATAYTQALHWASAMTGEAPATNQT"
     gene            complement(6688..7959)
                     /locus_tag="VHE33_06635"
     CDS             complement(6688..7959)
                     /locus_tag="VHE33_06635"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HVT97166.1"
                     /translation="MPSLASPPEAAPRATTPAPPITVPSHFLGRQPILDAERRLFGYE
                     LLYRTSAESHSSGDPEEATREVIDHWLLLIPDTSRSTSFVNCTRSAVTDGLVTLLPPS
                     ATVLEIPEQVDPDGELLSACLTLKLQGYRFALDGFLPRAGRAPLLALADFVKVDFQTC
                     GYDLRREVYAMAAGTGAHLVAEGIENDLQFRIAVSEGCTLFQGYFFSQPVILASHTVP
                     QNYFVYLKLLAELHRDPVHLRKVEKLIAGDASLCYRILRVANSALQGHPGVVSTIREA
                     LLMIGEDSLRRMVTVAVAGALSHQRPTALLSMTLARAQFCELLAPSLSFDAAQLYLLG
                     LLSLLDVLFEMPLSRILETIPLSPAMKGALLGDDGIASRALSLVRALEACDWHRCEQI
                     QAQLGIAEGTIAAMYAQSLRWASAMLGETSS"
     gene            complement(8071..9321)
                     /locus_tag="VHE33_06640"
     CDS             complement(8071..9321)
                     /locus_tag="VHE33_06640"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HVT97167.1"
                     /translation="MLSVPVENPISAAHSSSHPPRFVGRQPIVDEKGRLFGYELLSRP
                     KPVDVPPADPEAATREIVDYWSMLLPAHDAGFAFVNCTRASLMDGTVTLLPSRNTVLE
                     ILEDIEPDAELIASCRALRKQGYRLALDDFAPVASRAPLIELADFIKIDFLACDTQMR
                     REIYSMARGSRVRFLAEKIETREHRDIALSEGCTLFQGYFFSRPVTVASPALPQSHFI
                     CLRLLTALHRRPTDLGEIERLVMSDASLTYRVLRLANSALYGLTSKVTSVRGALIMVG
                     DDAVRRMVSVAVAGTLAGNRLTPLLSMALIRARFCELLAPHIGAPPDELYLLGLLSLL
                     DVLLETPIERILEALPIDPEMKSALLGKPSRRLCSLSLVRCLETCDWTECEAVVRSAG
                     MNEDAVAALYVEAVRWASSTLQTS"
     gene            9527..10723
                     /locus_tag="VHE33_06645"
     CDS             9527..10723
                     /locus_tag="VHE33_06645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HVT97168.1"
                     /translation="MAQGRTSHQLRKFRFARPQKIAAVLLLVLLAECLWVSARQQLSE
                     TDYRYARCGREMWERPSPLAGYFTTCGNIHDGTLAYRVAGLPLTVERILRGQARSSSM
                     WDMRFEAREVKLLLRLPFMLAGLALGACLWWVTRRLFGNAGGYISLALYCCMPEVVRA
                     STTPNNEILAAFGVFSTIYLAIGVAHALQGPPRKWRPRIVLLTLALGFTAAAHLAAFA
                     VALAFTVVFMAWVAEGRRAYIPTLLIVWVVGAFLLLFASYAFHPDAFSYVFRSAAGRM
                     WLSMDGARWFFSNPANAAFTMAVLAALALYVGSRRSRYFGNTAPLLVAVPLMVLVTTG
                     VESKPWLWGLPFLIAFVGGVFADALETRGRKAFYWATAVVLAAQAGLCGAWLWGLARA
                     GALRVP"
     gene            10943..11869
                     /locus_tag="VHE33_06650"
     CDS             10943..11869
                     /locus_tag="VHE33_06650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT97169.1"
                     /translation="MQNRLGVSVTPSHFYFPVPSLKSFNGKDWRAPRPCSGVDFRLDE
                     QIARLRNDLTPFTHERTFGESNGDGRRQFHFNNGFFERIDAEVAYAFVRKRKPKRVIE
                     VGSGNSTLVMAAAMQANAAEGHPGELTSIEPHPAQYLKDGIPGLTRLMEMPVQKVPLS
                     VFKELQAGDILFIDSSHVVSIDSDVLYECLRILPELAPGVLIHFHDIFTPLDYPEKFV
                     KTNLCFWGEQYMLEAFLSFNSAFKVVWSSSAMQQYHPDVLREVFPGWEGSFARMPDDL
                     KVFAPTLDGKNVWPCSFWIERTEAQAQTAAAA"
     gene            complement(12017..>13539)
                     /locus_tag="VHE33_06655"
     CDS             complement(12017..>13539)
                     /locus_tag="VHE33_06655"
                     /inference="COORDINATES: protein motif:HMM:NF019880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HVT97170.1"
                     /translation="TVAQKSGIPLPKREFSSPEEAAEHRVRGKLLDLHEAATAWFEEQ
                     LRSPEAATAREYLTGRGLNADGQAKFRIGYAPDSFRALGERLQSLADPDTLRQSGLFS
                     WKEQEDGSPGPIYSRFRKRIMFPIANEAGRVIAFTARTLETGEKAGPKYLNSPETALY
                     SKSHVLFNLDKARQAIRQDGGAILVEGQMDCISVYLAGIPNVIATSGTAFTESQTRLL
                     RRYATRVIVNFDPDTAGANAAEKSISLLTEEGFDVRVLTLEGGLDPDRFIRERGARAY
                     VDALRASPTFQSYLTDRARQLFPSRDPKSKADALNFLLPHIRRIPNRIARDAFAIDAA
                     QALGIDSGLVRAELKEAAAKRRDSLASQTFPLTHAERILLQAFSAPRSSDCYIIAEGN
                     WHQHAHELAALRADVRAMVEQLRLRPDGADPLIAITDPAHRQMLAELFLSIHDTEPTS
                     DDVDAAILAIRRQAIEHEQRQLRIDLDRAERAGKPDDVLRLTRSIQEATQRLREID"
BASE COUNT         2545 a         3412 c         5167 g         2415 t
ORIGIN      
        1 tggcaggggg gattgttgac gcgagccatc attctgattc ggatcctggt cggatgggtc
       61 ttcgtttccg aggggatcca gaagtttctg tttccggcgc tgggcgtggg gcgtttcgcg
      121 aagatcgggt tcccgcatcc ggggatcctt gcaccgatcg ttggaatcgt ggaaatcgcc
      181 tgcgggttgg cggtgattct ggggctgtgg acgcggctgg cctcgatccc gctgctgatt
      241 gtgattggca cggccatcgt cacgaccaag atcccggagc tgcatcgggc gggcgagggc
      301 ttttggttca tgctgcacga cgcgcgaacc gacttcagca tggtgctggg tctggtgttt
      361 ttgctcgtcg tgggcggcgg gaccctggcg atgacgcgat cgcgatagcc tcgcggtctc
      421 cgacaggtta agaatgcggc aagcggttcg ctggttcagg gtagttcttc gcatcactca
      481 atgccagcct ggttgcgcct gccgatccac agtaacgaat tgatcgccgt cacttagaaa
      541 ccagcaacga agcaaaaacc gaactgttcg tcacagattg aagaaactcg acggtgacgc
      601 gaatcgtatt tagaacaggg caggactttt tggggtcttc ccacagattc ttgcctcctc
      661 atgaagcggg ctgtccttgt ggcggcccgt ctttttctgc gccgagtcca ggtgctggac
      721 gtgagttcgc gaatttgcat ccgtcttttt gaaggtcagt agaatttcag accatgaaga
      781 agagcgaagc ggccgaggtg ctgtcgattg gcgggcatga gattgcgatc acgcatccgg
      841 acaaactgta cttctcaaaa caggtcaggc tgacaaagat cgaactggtg cgctactacc
      901 tgtcggtggc ggagggagcg ctggcgggag tgcgcgatcg tcccctggtg ctgaagcgat
      961 tcgtcaacgg cgccgaggca gaggcgttct atcaaaagcg cgcgcccacg ccgcggccag
     1021 actggctgcg gacggtcaca ctctcctttc cgtcgggaag aacggcggag gaagtggtgc
     1081 tggacagcgt agcgggactg gtgtgggtgg ggaatctggg ctgcattgag ctgcacccgc
     1141 atgcggtgcg cgcgagcgat ctggatcatc cggacgagct gcgcatcgat ctcgatccgg
     1201 ggccaggggt gggatgggat gacgtgcgca aggtatcgct cgaggtgaag gcgttcctcg
     1261 aagaagtggg actgcgcggc tggccgaaga cgagcgggtc acgcgggatg cacgtgaata
     1321 tccggatcga gccgcgatgg agctttgagc aggtgcggca ggcggcgctg gcgttatcgc
     1381 gggagatcga gcggcgggtg ccgcagctgg caacttcaaa gtggtggaaa gaagaacgac
     1441 acggggtctt tctcgattac aaccagaacg cgaaggatcg gaccacggcg tcggcgtatt
     1501 cggttaggcc ggtgccggat gcgcgcgttt caacgccgct gcgctgggag gaagtgacgg
     1561 agtgcgatcc cgcggacttc acggtgttca cggtcccgga gcggtttgcg aaaatcggcg
     1621 atccgcacgc agaaatggac gcgagtgcgg gctcgctgga gaaactgctg gagtttgctg
     1681 cgcgggatga agcgtcagga ctgggggacg cgccctggcc gccgcatttc cgcaagatgg
     1741 cgggcgaagg ggcgcgggtg gctccgtcgc ggcagaaaaa ggcgtctccg tcagttgcga
     1801 aaaagccgcg ggagaagatg ccgctggtgg tggtggcgaa ttctcccgac aaggatgcgg
     1861 ctctggctgg cctggaacgc tggaaggcag cgcacgcgga tgcggcaaag catcttgctg
     1921 tggacgatgt gctggtggat tcgatgcggg gccgatcgtc gacgtggacg cgcattcggg
     1981 tgaacctgcg gcatgtgccg gaggacctgc ggccggcaca ggagacgccg gacccggatg
     2041 aggatccgac gcgggagtgg cggtcgcgga cgagaaagaa gtccgaatag acatcgtggc
     2101 ttccatatct gccaacagcg cgcagatatg atcccctgcg cgatgatgat tccgatgccg
     2161 ggcctcaaga cccttttttc cctgcctctt tttccccggc ctgaaggccg gggcttctat
     2221 cgtcgtgccg ctgacgcgcc acgttagcgg ttcgtcggaa agatagcttt tagttcttcc
     2281 ggcggcacga cctcaagttg ctcataggtg cagtcctttg gttctttgtc cttgcgccag
     2341 cggcggaact gcgccatgtg gcggaagcgg ccgctctgca tgtgttcgta agcgacctcg
     2401 gcgacgagtt cgaggcgcaa gggctcccag gaaaggtctt tgccctgact ccagcggctt
     2461 tgaccgccgg ggcggcgaga ggcgacgttc gcctctttgg aattctctga gtcgccttcg
     2521 gaattttcgg cgctgccggt ccagtccgcc cagggatggt ccttcaacgc attcttgcgg
     2581 tagggctcga ggaattcgac cagctcgatg cgcttctgca tggggaagct gccgcagacg
     2641 ccgacatgct gcagcgcgcc gctatcgtca tagagaccga ggagcaggga tccgatggca
     2701 ggctgtgtgc ccttgcgata ccagcggaag ccggcgacga cgcaatcgca gtcgcgctca
     2761 tgcttgacct tgaacatggt gcgcttgtcg ggctcataga ggccttgttt tgacttggcc
     2821 atgacgccat cgagaccggc gccttcgaag cggccgaacc agtcgccggc ctgcgctgga
     2881 tcgtcggtgg cgggggtgat gtggagcggc ggcttggcgc ccgagagaat gctttcgagc
     2941 ctggcgcggc gttcgtggaa gggcttgccg gtccagtcgt gcgcgccttc agcgagcaga
     3001 tcgaagaaga cgatggaggc gggagtttgc tggctgagca gcttcacgcg cgacgctgcg
     3061 gggtgaatgc gcaactgcag agcgtcgaaa tcgagcacgc cgttgcgcgc gatgacgact
     3121 tcgccgtcga ggacgcacgc gcgcgggagc tgttcgagga tcggttcgat tagctcgggg
     3181 aaatagcggt tcagcgattt ggagtcccga ctctggataa ggacttcatc tccgtcgcgg
     3241 aagatgaggg cgcgaaaacc gtcccacttg ggctcgtaga tccattcggc gccggttggc
     3301 agtttggcga ctcgtttgga gagcatcgga aggacgggcg ggttgacagg aaggttcatg
     3361 agttttgatt tgtgagttga gatgcggctc gcgaaattac ggtgaccggc ctagaagctg
     3421 gcgccgccgg tgttgggctg gttgctgagg acttctttgc cgccgctgcg gccgatgaca
     3481 taaacattgc cgggaagggt ggggacatcc tcgagtgggg tccagagcca gacgcgcgcc
     3541 ggactctgcc agattccgca caggccgcga tggttgatga agaagggctg cggaggaggc
     3601 cccacgtagg aaagcacggt ggtcttcgtc gtgaccggca cgttgccgct gcaggaagcg
     3661 ggcacggact cgttgggcgc gacgtggttg agcagcagaa cgttgcgctc gaggtagaag
     3721 acgaaagagg aggcgtcgtc gatgtgcccg ttgaccacca ccagatcggc gggctgcact
     3781 tcggggtgaa tggcttcggc gaggatctcg gaggagagga ccggggagaa ggtgttgagg
     3841 gcgagatgcg ccgcgatgag gatggccaca atcatgccgg cgaggaagca attagccatg
     3901 cgggcgttgt cacgcaaccg gtagaagagg gagacggaga cgccgaccag ggtggcgatc
     3961 agggtgatcc agagcgggcc gcgaaccgcg cccatggtgt tgcgggtgag gtcgaggaaa
     4021 tacgcgaaga agacggcgtg atggcccgga tgggagcgga tcagggtggc gatgtcgacg
     4081 ccaggcgcgg gaacgggcgc gacgatgagg taataggcga tgagcgcgga ggcggcgagg
     4141 ccgaagaaga aaagaatcca ggcggcgatg cggccttcgt gatggagttg cgcttcgtct
     4201 tcggcgagcc atccgccggc gagcagcgcc atgggcggga gtgcggtgag caggttgaat
     4261 tcctggcgcg ccgtaaagct gaagaagagg aggacggtca ggatccagat cagcgagaaa
     4321 cagcgcgcct ggcggcggcg gtcgggttcg ggtgaagcgc cgatgcgaag ggcgcggaga
     4381 gagtaggcgc accagggaat gatccagagc aggagcagcg gccagaagac gatgagcggg
     4441 acgcggccgc cggtgaagcg aggcaggagg ttcatcaaat cctgctggcg gagagcgtgg
     4501 cgggcggcga aatgccaggg aagatacaga accaggaaag tgagaatcgc gggaatgggg
     4561 tgccagcggc gcaggtgccc gagattgtgg gtgaaggcaa gataaagcac tgcgatgatg
     4621 agcggcagca gcaggccgga aagtccctga gtaaggacgc cgagcgcgca acaggtggcg
     4681 aagccgagcg cagattcgag caggcgcttt tgctgcgtca gggagcgcca gaagaaattc
     4741 agggcgagcg tggtccacag gcagaggaat acgtcgcgga tgagaaggtg gccgaagagg
     4801 aaaacgccgt aggaggtgag gagggtgagc accgcataga aagcggcggc cggagagtgg
     4861 aagatgcggc ggcccagaga gaaggtcgcg atgcagaggc cgaggatggc gatggcgaca
     4921 gggaggcggg cggcccagtc cgaaacattg aagatgccga aactgacggc gacggtccag
     4981 tagaggagcg gcggatggtg ggggtagacg atgccgttgg cgaagggtgt gagccagtgg
     5041 ccgccggtga gcatctcccg ggcgatttcg gcgctgaggg catcggagcc atcgagcagc
     5101 ggtggcttga agagggagaa ggagccgtag aggagcaccc agattccgag cagggggaag
     5161 acgaagcgct tcgccgtgcg gagggtggtc atggactctt agggtacagg gtacaggggg
     5221 cagggtacag ggggcacagg tcgcagggca caggtcgcgg ggctgggtcg gcgtcaggtc
     5281 tggttcgtcg ccggggcttc gccggtcata gccgaggccc agtggagggc ctgcgtataa
     5341 gcggtggcga tgacgccttc ggccagaccg agtttgtgct ggatttcttc gcagacgccc
     5401 cagtcgcagg attcgaggcc acggaccagt gcgagagtga gaccggcagg actggcgtcg
     5461 ccggcgaggg cgagcttcat ctcgttgctg atggggatgg attgaaggat gcgcgagaaa
     5521 ggcatctgga gcagggcgtc gaggagggag agcatgccga ggagatagag ctcggcggga
     5581 tcttcgcgaa aagagggggc gagcagctca cagaaacggg cgcgggtgag gatcatgggg
     5641 atcagggcgg aggactgctg agcgccgagg gcgccggtga ccgcgacggt gatgaggcgg
     5701 cgaagagcgt cctctccaat catcaggagg gcttcggcga tggtcgtgat cgcgccggga
     5761 tgatgctgca gggcggagtt cgccagacga agcacgcgaa agcacagggc tgcgtctccc
     5821 atgatgatct tttcgagttt gcgcaggtcg gtagagccct gccggagctg ggcgagcagg
     5881 ctgagataga ccgtgtgatt gcggggaatc gcatgcgact ggaccaggat gggctgagag
     5941 aaaaaatatc cctggaacag agagcagcct tcggcgatgg cgatgcggag ctgggcgtcg
     6001 gattcgattt tttccgcgac cagttgcgcc ggggtgccgg aggcgagcgc gtaaatctcg
     6061 cggcggacga gaaagtcggc ggtcatgaag tcgactttga tgaagtcagc taaagcgaga
     6121 aagggagcgc gggacggacg ggggaggaag ccgtcgaggg caaagcgata gcctttggat
     6181 ttcagcgcaa ggcaggcatt gatgagggca ggatcggggt cgatgtcttc gagaatctcg
     6241 agaacggtgg attcgggagg cagcagggtg atcaggccgt ccacaatagc gttgcgcgtg
     6301 cagttgacga aggccgcgcc ctggcggtga tccgggatca gcatgagcca gtggtcgacg
     6361 acttcgcgtg tggcgtgctc gggatcgccg gagaagtggt cggtttttcc ctcgcggtag
     6421 aggagctcgt agccgaagag ccggcgctcg gcgtcgagga tcggctgacg gccgaggaaa
     6481 cggtgggggg taaggttcgc tggtgcgtga gccccgggtt ggggtggggc ttcgaatttc
     6541 ggcgaggcaa gagcagacat ggaataagcc ttcgccggct ctgccagcga tcactttcgc
     6601 ttatcggcgg agctgaggcg aagagtcaga ggtgactgga aaagaagcag ccggtcctgg
     6661 tggcggatgg cagtcccggg aaagggctca ggaggacgtt tcgcccagca tggcggaggc
     6721 ccagcgcagg gactgggcgt acatggcggc gatggtgcct tcggcaatcc cgagctgagc
     6781 ctggatttgc tcgcagcggt gccagtcgca ggcttcaaga gcccggacga ggctgagggc
     6841 gcggctggca attccgtcat cgccgaggag cgcccctttc atcgcagggc tcaaaggaat
     6901 ggtttcgagg atgcggctga gcggcatttc gaaaagcaca tcgagcagcg acagcaaacc
     6961 gaggaggtag agctgtgccg cgtcgaagga gagcgaaggg gcgagcagct cacagaactg
     7021 agcgcgcgcc agagtcatgg agaggagtgc ggtgggacgt tggtgggaga gggctccggc
     7081 gacggcaacg gtgaccatcc ggcgcagcga atcttcgccg atcatcagga gggcttcgcg
     7141 aatcgtggag acaacgccgg gatgaccctg gagcgccgaa ttagcgacgc ggaggatgcg
     7201 gtagcaaaga gaagcatcgc cggcaatgag cttttccact ttgcgcaggt ggaccggatc
     7261 gcggtgcagc tcggccagaa gcttcaggta gacaaagtaa ttctgcggaa ccgtgtgcga
     7321 ggcgaggatg accggctggg agaagaagta gccctgaaag agggtgcagc cctcgctgac
     7381 cgcgatgcga aactgcagat cgttttcaat cccttcggcc accagatggg cgccggtgcc
     7441 ggcggccatg gcatagacct cgcgccgcag atcgtacccg caggtctgga agtccacttt
     7501 gacgaaatcc gccagcgcaa gcaggggagc ccgtcccgcg cgaggcagaa agccatcgag
     7561 ggcgaaccgg tagccctgca gtttgagggt caggcaggcc gagagcagtt ctccatcggg
     7621 atcgacctgc tcggggattt caagaaccgt ggctgagggc ggcagcagcg tgaccagtcc
     7681 atcggtgacg gcgctgcggg tgcagttgac gaaggaggtc gaacggctgg tgtcgggaat
     7741 gagcagcagc cagtgatcga tgacttcacg ggtggcttct tccggatcgc cggagctgtg
     7801 gctttccgca ctggtgcgat agaggagttc gtagccgaag agccggcgct cggcgtcgag
     7861 gataggctga cggccgagaa agtggctggg aacggtgatg ggcggcgccg gggtggtggc
     7921 ccggggcgca gcttccggcg gagaggcgag ggagggcatg cagtcgaaat cgccgggaat
     7981 tcgatttcct gggattcaac tggtgtatcg gcggggaacg gacgaagatg aggccgatcc
     8041 gcaccgccgg gcccgtcgtg gaaaagacaa ctaagaggtc tgcagcgtcg aagaagccca
     8101 gcggacggct tcgacgtaga gcgcggccac cgcgtcttcg ttcatgccgg ccgagcgaac
     8161 gaccgcctcg cactcggtcc agtcgcatgt ttcgaggcaa cgaaccagac tgagggagca
     8221 cagccgccgg ctcggtttgc cgaggagagc ggacttcatc tcgggatcga tgggaagcgc
     8281 ttcgaggatg cgctcgatcg gggtttcgag caggacgtcg agcagggaaa gcaggccgag
     8341 gaggtagagc tcatcgggag gcgcaccgat gtgaggcgcg agaagttcgc agaagcgggc
     8401 gcggatcagc gccatggaga ggagcggggt aagacgattg ccggccagag tgcctgcgac
     8461 agcgacggaa accatgcggc gaacggcgtc atcgccgacc atgatcaggg caccgcgcac
     8521 cgaggtgact ttgctggtca gcccatagag ggcggagttg gccaggcgca ggacgcgata
     8581 ggtaagcgag gcatcggaca tgacgaggcg ctcgatctca ccgaggtcgg tgggacggcg
     8641 atggagcgcg gtcagcaggc ggagacagat gaagtggctt tgcggaagcg cgggcgaagc
     8701 gacggtgaca ggccgggaaa agaaatatcc ctggaaaagg gtgcagcctt cggagagagc
     8761 gatgtcgcgg tgttcccggg tttcgatttt ctcggcgaga aaccggaccc gggagccccg
     8821 ggccatggag taaatctcac ggcgcatctg cgtatcgcag gcgaggaaat cgatcttgat
     8881 gaagtcggcg agctcgatga gcggagcgcg cgaagcaacg ggcgcaaagt catcgagagc
     8941 gagacggtag ccttgtttgc ggagcgcgcg gcagcttgcg atcagctcgg catcgggctc
     9001 gatatcttcg aggatctcaa gaaccgtatt cctgctcggg agcagcgtaa ccgtgccgtc
     9061 catcagagaa gcgcgcgtgc agttcacgaa ggcgaaccct gcgtcatggg caggcaggag
     9121 catcgaccag taatcgacga tctcgcgggt tgcggcttca ggatcagcgg gaggcacgtc
     9181 gaccggcttg ggccgggaaa ggagttcgta gccgaagagg cgtccctttt cgtcgacgat
     9241 gggttgacgg ccgacgaaac gtggggggtg ggaagaggag tgtgcagcgg agatcggatt
     9301 ttcgacgggc acggagagca tggaacggtc aggggaaatt gtagatttgg cggagttagc
     9361 ggaacatccc tcggacaggg gagtagcgca gcgtttgctt tcgagccgct cctggacaat
     9421 gagaaatcgg gggcggccgc gctgcccggc gtgagataag aaacgagcga gcacccgatc
     9481 gcggagctct ggggcgaggg gtttcgatac acttgcagaa gtctcgatgg cacaagggcg
     9541 aacgagccac cagctgcgaa aattccggtt tgcacggcca cagaagatcg cagcggtgtt
     9601 gctgctggtg ctgctggcgg agtgtctgtg ggtgagcgcc cggcagcagc tctcggaaac
     9661 cgactatcgc tatgcgcggt gcggacggga gatgtgggag cggccttcgc cgctggccgg
     9721 ctacttcacg acgtgcggga acattcacga cggaaccctg gcctatcgcg tggcggggct
     9781 gccgctgacg gtggaacgaa tcctgcgagg gcaggcgcgc tcgagctcga tgtgggacat
     9841 gcgattcgag gcgcgggaag tgaagctgct gttgcggctg ccgttcatgc tggccgggtt
     9901 ggcgctgggc gcgtgcctgt ggtgggtgac gcggcgcctg tttgggaatg cggggggtta
     9961 catttccctc gcgctgtatt gctgtatgcc cgaggtggtg cgggcgtcga cgaccccgaa
    10021 taacgaaatt ctggcagcgt tcggggtgtt ctcgacgatt tatctggcca ttggggtggc
    10081 gcatgccctg caggggccgc cgaggaaatg gcgtccgagg attgtgctgc tgactctggc
    10141 gctggggttc acggcggcgg cgcatcttgc ggcgtttgcc gtcgcgctgg ccttcaccgt
    10201 ggtctttatg gcctgggtgg cggaggggcg gcgagcgtac attccgacgc tgctgattgt
    10261 ctgggtggtg ggcgcgtttc tcctgttgtt cgcctcgtat gcgtttcatc cggatgcgtt
    10321 cagctacgtg ttccgcagcg cggcaggccg gatgtggctg tcgatggacg gggcgcgctg
    10381 gttcttctcg aatccggcga acgcagcgtt caccatggcg gtgctggcgg cattggcgct
    10441 gtacgttggg tcgcggcgat cgcggtattt cgggaatacg gccccgctgc tggtcgcggt
    10501 gccgctgatg gtgctggtaa cgacaggggt agagtcgaag ccctggctgt gggggctccc
    10561 cttcctgatt gcttttgtgg gaggggtttt tgccgacgcg ctggaaaccc ggggaaggaa
    10621 ggcgttttac tgggcgacgg cggtcgtgct ggcggcacag gcaggtttat gcggggcctg
    10681 gctgtggggg ctggcgcggg ccggtgcgct tcgcgtcccg taagatcaat tggcgccgtt
    10741 acctcgggat cttgccgggg gagagacctt tctccacggt ttcacagagg acgaggcagc
    10801 tttccgggct cttcgacctt ctaagaacct gaaagatggg cacgccaaac cggatgtgaa
    10861 gtcgggggcg atgaggtcgt gtgtgagcag catccttcga agcgccagag tccggacgac
    10921 gctggcttcc ttgtttgagg taatgcaaaa tcggctcggt gtgagcgtta cgccttcgca
    10981 tttctatttt cctgtaccga gccttaagtc gttcaacgga aaggactgga gagccccccg
    11041 gccctgctcg ggcgtggatt ttcgcctgga cgagcagatt gcacggctgc ggaacgatct
    11101 gactccgttt acgcatgaga gaacgttcgg cgagagcaat ggcgacgggc ggcgccagtt
    11161 ccacttcaat aacggatttt tcgaacgcat cgacgcggaa gtggcctatg cgtttgtgcg
    11221 gaagcgcaag ccgaagcggg tgattgaggt cggaagcggc aactcgaccc tggtcatggc
    11281 ggcggccatg caggcgaatg cggccgaggg acatccagga gagctgacca gtatcgagcc
    11341 gcacccggcg cagtatttga aggatgggat tccgggcctc acacggctga tggagatgcc
    11401 ggtgcagaag gttccgctga gcgtgtttaa ggaactgcag gctggagaca ttctgttcat
    11461 cgactccagc catgtggtgt cgatcgacag cgacgtgctg tacgagtgcc tgcggattct
    11521 gccggagctg gcgccggggg tgctgatcca tttccacgac atcttcacgc cgctggatta
    11581 tccggagaag ttcgtgaaga cgaacctgtg cttctggggc gagcagtaca tgctggaggc
    11641 cttcctgtcg ttcaactcgg cgtttaaggt ggtttggtct tcgagcgcga tgcagcagta
    11701 tcatccggac gttctccggg aggtgttccc tgggtgggag ggaagcttcg cgcggatgcc
    11761 ggacgatctg aaggtgtttg ccccgacgct ggacgggaag aatgtgtggc cgtgcagctt
    11821 ctggatcgag cggacagagg cgcaggccca gacggcggcc gcggcgtaga cggaggagca
    11881 gaataacgga gaggcccggc gctggagtca gcgtgcgggc cttttctctg tggcgagcga
    11941 tttattgtgt ggcttgagat cttcgtagga acggacgccg gccggtgccc gtcgctcgcc
    12001 atcggatgaa tccggatcag tcgatttcgc ggaggcgctg cgtcgcttcc tggatggaac
    12061 gcgtaaggcg aagcacgtcg tcgggtttgc cggcgcgctc ggctcgatcc agatcgatgc
    12121 gcagctggcg ttgctcgtgt tcgatggctt ggcggcggat cgcgaggatg gcggcgtcga
    12181 catcgtcgga ggtgggctcg gtgtcgtgga tagagagaaa gagctcggcc agcatctggc
    12241 ggtgggcggg atcggtaatg gcgatgagcg ggtcggcgcc atcggggcgc aggcggagct
    12301 gctcgaccat ggcgcggacg tcggcgcgga gggcggcgag ttcgtgcgcg tgctggtgcc
    12361 agttgccttc ggcaatgatg tagcagtcgc tggagcgcgg tgccgagaag gcctggagca
    12421 ggatgcgctc ggcgtgggtg agggggaagg tctgcgaggc gagggagtcg cggcgtttgg
    12481 cggcggcttc tttgagttcg gcgcgaacca ggccggaatc gatgccgagc gcctgggcgg
    12541 catcgatggc gaaggcgtcg cgggcgatgc ggttggggat gcggcggatg tgggggagca
    12601 ggaagttgag cgcgtcggcc ttggacttgg ggtcgcgcga gggaaagagc tggcgggcgc
    12661 ggtcggttaa gtacgactgg aaagtggggg aggcgcggag tgcatcgacg taggcccgcg
    12721 cgccgcgttc gcggatgaag cggtcggggt cgaggccgcc ttcgagggtg aggacgcgga
    12781 cgtcgaagcc ctcttcggtg agcagggaga tggacttctc ggcggcgttg gcgccggcgg
    12841 tatcgggatc gaagttgacg atgacgcggg tggcgtagcg gcgaaggagg cgcgtctgcg
    12901 actcggtgaa ggccgtgccg ctggtggcga tgacgttggg gatgccggcg agatagacgg
    12961 agatgcagtc catctggcct tcgacgagga tcgccccgcc gtcctggcgg atggcctgac
    13021 gggccttatc cagattgaag aggacgtggc tcttcgagta gagcgcggtc tcgggtgagt
    13081 tgaggtattt gggaccggcc ttctcgccgg tttcgagcgt ccgcgcggtg aaggcgataa
    13141 cgcggccggc ttcgttggcg atggggaaca tgatgcgctt acggaagcgg gagtagatgg
    13201 ggccgggact gccgtcttcc tgttccttcc aggagaagag tccggactgg cggagagtgt
    13261 cgggatcggc gagggactgc aggcgctcgc cgagggcgcg gaaggagtcg ggcgcgtagc
    13321 cgatgcggaa tttggcctgg ccgtcggcgt tgaggccgcg gccggtgagg tattcgcggg
    13381 cggtggcggc ttcgggggag cggagctgtt cttcgaacca ggcggtggcg gcttcgtgga
    13441 ggtcgaggag cttgccgcgc acgcggtgct cggcggcttc ttcaggggag gagaattcgc
    13501 gtttggggag agggatgccg gatttttggg cgacggtgc
//
LOCUS       DASZYN010000256        11192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_972041, whole genome shotgun sequence.
ACCESSION   DASZYN010000256 DASZYN010000000
VERSION     DASZYN010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 11192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11192
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972041"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..732)
                     /locus_tag="VHE33_06660"
     CDS             complement(64..732)
                     /locus_tag="VHE33_06660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HVT97171.1"
                     /translation="MPSASTTDPGATSLGWRLANVLAIASPYAVAGALFLVLYLSRAP
                     APPSFETSEATAPAASASGASVPVEADTTGTAAAAATPSQPAPQPLPVSHAKQAKPPA
                     PVHPPAMEPQTSSHDAVRGQPVRVDPAVAATMKLSGAPPSYPAIARAAGVEGTVVVAL
                     TIGPKGSVLDAHAVSGPPLLQPGTVFAVRSWRYRPWLAYGNAIPFETEVSIHFEINSP
                     SSGR"
     gene            822..1583
                     /locus_tag="VHE33_06665"
     CDS             822..1583
                     /locus_tag="VHE33_06665"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HVT97172.1"
                     /translation="MSGVKATFSIPVGEGTLHCEAVVPEGPTTLTQLLPIIRNLESAI
                     GARVAQEAESAGRPITCGPGCGACCRQLVPVSLFEAESLTNWMRTLTDEQQSLLQERF
                     HRALLALREAGVIDKILSDEWIAGGEPVTQLAIDYFHAGVPCPFLENESCSIHPIRPL
                     SCREYLVTSPPVLCQDPAAHPVAGLQFPLKLSRTLYNLGRQIEQDPRGWIPLVFLPAW
                     GKSGFRPGEHVAGTGEELLRRFLEEAMGAPPQPEQ"
     gene            complement(1637..3346)
                     /locus_tag="VHE33_06670"
     CDS             complement(1637..3346)
                     /locus_tag="VHE33_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HVT97173.1"
                     /translation="MALEAGQRIGDYEVQSLLGTGGMGRVYRVRNVISDRVEAMKVLL
                     PDYASEPELAARFTAEIRTLATLEHPNIAQLRTAFQFENQLVMVMEFVEGSTLDQLAS
                     QSRIPLNLVLEYAMQVLSALSYAHGRGITHRDIKPSNIMVTAHGLAKLMDFGIAKSGG
                     DLQLTRPGTTMGSVYYMSPEQVRGGTVDARSDLYSFGVTLYELVTGNRPFQADTSFTV
                     LNAQLNETPKPPIEVNPSLPPALNSIILCAMAKEPTDRFQSADAFRNAIKATQDALQF
                     PTTATPQPQGAFQASPQPQQQPFRPQSQPNQPQGGFAPVAVPPIPQPASKNRRTLWMA
                     LGAVAALIAIAAAATLLPRMLATHAGQRAETASANVSDAQPQSAATTSSSTPTPAATA
                     TPTPSPQDATPAPPPDVTQPAAASPTPSPSAEAATPTPASENHSPSHRATAGGHQREP
                     YTSRGPASAPANDVPAQSASAPASANAAPSAQEFRTARDRYANLGARADAAKSGVQSI
                     RSQQQAQGLDLRGDILAALSSLNNDLNESNLALTQNDLETANQYMDRADRELGILEKF
                     LGR"
     gene            3611..4375
                     /locus_tag="VHE33_06675"
     CDS             3611..4375
                     /locus_tag="VHE33_06675"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HVT97174.1"
                     /translation="MSVRNALKIDAFGISDVGCQRTNNEDSFSYDLASQLFVVCDGMG
                     GMAAGELASGTAVQELLRSFTASDPGQPIEERLYDAIADANRSVFALAEGSAELKGMG
                     TTLVAACVEGEKLVVANVGDSRAWFLRNGSCAQITHDHSFVAEQVRIGAMSPEQAGVS
                     PLQSLLTRAIGQAEAVEPDIFTAPLEPGDILLLATDGLSRYADTDTIAGIVLNSATLE
                     QACQALIECAKSQGAVDNVTCLLLQVIEGEPETSAP"
     gene            complement(4372..7800)
                     /locus_tag="VHE33_06680"
     CDS             complement(4372..7800)
                     /locus_tag="VHE33_06680"
                     /inference="COORDINATES: protein motif:HMM:NF018470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HVT97175.1"
                     /translation="MLSILVSALVLIVAILLAWAAGPLMGLTGTALILLRILLLVIGA
                     GVAGIILFLYFREKKRDSAAAGLPGGTDLDTILREAERQLATAQRTGPKTLDTLPLLY
                     VLGEANSAKTTTVLKCGLDPELIAGQVYRNQEIAPTSVANVWYTRECVLVEAGEAVRK
                     NPALWSKLIRKTRPKLVRSAMGKQAPVRAAVFCVSAEQFLGASAPDAVMNAARGGNQM
                     LRDLARQMGTGIPVYVIVTKLDRVPSFTEYVRNLSPDEALQPVGMALQRSESGAGLYA
                     EQATKEITTSLDQVIFSLGEFRLELLTRENDAKNLDPVYEFPRELNKLRNALTTFLVE
                     LARPSHLNANPYLRGFYFSGVRAQVIEQVVASAAATPPPPADAGATRMFSIEQMRAAA
                     ANPAPQMVSQKVAQWCFLPRLFSSAILDDRAALAATSNSGRTHIFRRIALASLSFVLF
                     VWFICLVVSWANNSSLVQSIASAASALPSGSVPAGTLAPTRDLAALDALRQKIVQLEK
                     YQQDGAPLSYRWGLYRGDDLLKPARQIYFDRFRALLLANTQASLLSALGGLPGTPPAG
                     ADYSAAYNPLKAYLITTSNPDKSTVEFLSPVLVQYWENGRTPETDDQLRLARQQFDFY
                     AAQLARKNPYSIAADMSVVTHARTYLASFGGFERIYQQMLTAAGKVAHPIDFNRDYPG
                     SGSTVVDARIIPGAFTHDGFDFMHDAILHPDRYFSGEAWVLGNQAPPSLDRASVMQQL
                     TTRYQQDFQSEWRAWLHAAQVIRYHSLGDAAAKLQIVSNPSSPLLALFYTASHNTAVA
                     DPQIAKEFQPVQAVVAPQSAEKFVGSGNTNYVNGLLGLQSAVAQVAQDPTAATNPAAV
                     TPILTASASAHTAASQTSQAFNLDPQGHVDQTVLALLQAPINSVDEVVRGRGPQQANA
                     AGAGFCSAFAPLMTKFPFNPNSTVDATPAELSGVLQPGSGALWQFYDTTLKPLMIQEG
                     TTFVAAPNSPMRLNTDFLRFFNRVAGLSALLYPATPGGITFNAHILPSRGIQGVTLQI
                     DQQRISGSDVSHQFTWSPQTSQQSQLIATYATGTLPLLQFSGPWALLHLIDKGHVEQA
                     GNPVRLAYPLEVSNTPIVVNGTPLVVHLEFSGANANYLMPGGLSGIRCVSVVAH"
     gene            complement(7810..8496)
                     /locus_tag="VHE33_06685"
     CDS             complement(7810..8496)
                     /locus_tag="VHE33_06685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HVT97176.1"
                     /translation="MSVPRVNSLALCFQETLTAILRVRFQRQQVQDAESFRAHMRRSL
                     QGAMQDGRALGYSSETVQMAVFAVVAYMDESVLNLQSPVFAEWARRPLQEELFGGHLA
                     GETFFRNLRTLLSQPDSPENADALEIHCLCMQVGYRGRYALGDSGELHQLQRQAIAKI
                     QRVRGPAHLTPLVVRPEVRAVRTKDPWTRALLIAVCALAALVFVAFGGFDLVLGSGVA
                     HVQSSTFSAP"
     gene            complement(8493..9845)
                     /gene="tssK"
                     /locus_tag="VHE33_06690"
     CDS             complement(8493..9845)
                     /gene="tssK"
                     /locus_tag="VHE33_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HVT97177.1"
                     /translation="MRFLSRVVWSEGMYLSPHHFQTQSRYFEDSVAFLAASLWHEPWG
                     LLHAELDQKAIRNGDAALLYASGIFPDGLTFDIPGSDPAPKARTLADLFPATDAALPL
                     YLAVAGRRDNGFDFDPSDAANGTRFSVMQRVLRDETNGVDEREVALGRKNIRLLTEAE
                     LTPEMLAIPVARVLRDGRAHLVYDEDFIPPCLRLNASEPLMLLVKRLLDAVGEKSATV
                     SRSARRQGRFEAGTGALDVANYWFLHALHSALAPLQHLATTRHAHPEDVFLELSRLAG
                     ALCTFSIDSDPRQLPSYDHRDPGPAFRALDAHIRRHLEIVVPSNTVRLDFHPVEPYIQ
                     AAEVRDERCLRRARWIVGIRSSMGESDLLRQVPMLVKVCSSKFVPELVRRALPGLTLT
                     HLPVPPAAVRAEADMHYFSIETSGPCWEHILQTRSVGIYIPGEISHPVFEVIVVVEPS
                     "
     gene            10054..>11192
                     /locus_tag="VHE33_06695"
     CDS             10054..>11192
                     /locus_tag="VHE33_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97178.1"
                     /translation="MDQPKIPLGNPEPGQRNETENSGPASATGIFGTIAPSNPSPDED
                     LFASLARMNPSSAPATPPLDTEDAVKPSVEPEKPAQSPRPGEFTQMLQSLKKPESGSS
                     PQPAATPPQDLARIFRQISVERKPAPETPVYSPKPAGSPASPPAALSPSAAAAPQPPA
                     AGTPGSFTGMFQSLGGQLDANQPVPAPAAPAAATPAPSAAEARPASSPPGEFTQFFKA
                     VPSAAAPPAQQPPPGFPAQPVQKSPGAFTQMFSQPATPAAAPDPAFPTQLAQKNEPGS
                     FTQAFSQPTGPAAPSSPPAYSSPPAYQEAPKPQSDFWSGAIPPATPSSTPPGQGGFTQ
                     LFQSLNDEGPAPYKPPEAPFSPAPAAAPASAAAPGGGFTQLFRTLS"
BASE COUNT         2213 a         3363 c         3621 g         1995 t
ORIGIN      
        1 cgcctgttga cctctcgcca tcgccaaagc tgaaggtctc gccggggaac tctcccaacc
       61 ggcctaacgt cctgacgagg gggagttgat ctcgaaatgg atgctgacct cagtttcgaa
      121 agggatcgca ttcccgtacg caagccatgg gcgatagcgc catgaacgca cggcgaaaac
      181 agtcccaggc tgaagcaacg gcggtccgct gacggcgtga gcatctagga ccgagccctt
      241 cgggccaatc gtgagggcaa caaccaccgt gccttcgacg ccggcggcgc gggcaatcgc
      301 gggatacgac ggcggagcgc ctgaaagctt catggttgcc gcgaccgcgg gatccactcg
      361 cacgggctga ccacggaccg cgtcgtgaga cgaagtttgc ggctccatag caggcggatg
      421 aaccggcgcg ggcggcttcg cctgcttcgc atgcgacaca ggcagaggct gaggcgcggg
      481 ttgtgacgga gtcgcagcag cagccgctgt gcctgttgtg tcggcttcga ccggaaccga
      541 ggcgccgctg gcggaggctg caggggccgt ggcttcggat gtctcgaaag aaggcggcgc
      601 gggagcccgg ctcaggtaaa gaaccagaaa gagggccccg gccaccgcat agggcgatgc
      661 gatggcgaga acgtttgcta agcgccaccc cagggaagtt gctccggggt cggttgttga
      721 agctgaaggc acgcttacat ccggcaagca gggatgggca gaattgtacg ggatgttgcg
      781 tcggaagcaa gtcgggcgga tcgtcctgag acaatgggtg cgtgtccggc gtaaaggcta
      841 cgttttcgat cccggtagga gaagggacgc tgcactgcga ggcggttgtg cccgaaggcc
      901 cgaccaccct gacccagttg ctgccgatta ttcgaaacct ggaaagcgcc atcggtgcgc
      961 gtgtggcgca ggaggccgaa tccgcgggga ggccgattac gtgcggtccg ggatgcggtg
     1021 cgtgttgtcg tcaactcgtt ccagtgagcc tgttcgaagc cgaatcgctg acgaactgga
     1081 tgcgcacgct gacggacgag cagcagtcgt tattgcagga aaggttccat cgcgcgctgc
     1141 tcgcactacg ggaagctgga gtcattgaca agattctgag cgatgaatgg atcgctggag
     1201 gagaacctgt cacccaactg gcaatcgatt atttccatgc cggcgtaccc tgcccgtttc
     1261 ttgagaacga gagttgcagc atccacccca ttcgtccgct cagttgcaga gaatacctgg
     1321 tgacgtcgcc gccggtcctg tgccaggatc cggctgccca tccggtggcc ggtctgcagt
     1381 ttcccttgaa gctctcgcgg acgctctaca atctggggcg gcagatcgaa caggacccgc
     1441 ggggctggat tcccctggta tttttgccgg cgtggggcaa gagcggattt cgaccaggcg
     1501 aacacgttgc aggcaccggt gaagaattgc tgaggcgatt cctcgaagag gcgatgggcg
     1561 cgcctccaca gccagaacag tgatcgcgag ccgggtgcac cgaaccggtg aagtccgctg
     1621 cgaatccccg agagggctat cgtcccaaga acttctccag tatgccgagt tccctgtcgg
     1681 ctcgatccat gtactggttc gcggtctcga gatcgttctg ggttagcgcg agattcgact
     1741 cgttcagatc attgttcagg ctgctaagag cggcgagaat gtcgccgcga aggtccagcc
     1801 cctgcgcctg ctgctgggag cggatcgact gaacgccgct cttcgccgca tcggcgcgcg
     1861 cgccgaggtt cgcatagcga tcgcgcgcgg ttcggaactc ctgcgcggag ggtgcggcgt
     1921 tcgcggatgc gggagccgag gcgctttgcg ccggcacatc attggcaggc gcactcgccg
     1981 ggcctcgaga agtgtaaggt tcccgctggt gtccgcccgc ggtcgctcga tgagatgggg
     2041 aatggttctc gctcgccggt gtcggcgtcg cggcttcggc agaaggagaa ggcgtcgggc
     2101 tggcagccgc gggttgcgta acgtctggcg gaggagcggg cgtggcatcc tgcggagacg
     2161 gcgtcggcgt cgcagtcgcc gcaggcgttg gagtcgacga tgaagtcgtt gccgcggact
     2221 gcggttgcgc atcggagaca ttggccgatg ccgtctccgc ccgctgacca gcatgcgttg
     2281 ccagcatccg cggcagcagg gtcgcagccg ctgcgatcgc gatgagcgct gccacggcac
     2341 cgagagccat ccacaacgtg cgccggttct ttgaagccgg ttgcggaatg gggggcacgg
     2401 cgaccggcgc gaatccaccc tgcggttggt ttggctggga ttgtggccgg aatggctgct
     2461 gttgcggttg cggcgaagcc tgaaatgccc cctggggctg tggcgtggcc gtcgttggga
     2521 actgcagcgc atcctgggtc gctttaatgg cgttccggaa ggcatccgct gactggaacc
     2581 ggtcggtggg ctccttcgcc atcgcgcaaa gaatgatgct gttcagcgca ggcggcagcg
     2641 agggattcac ttcaatgggc ggcttcggcg tctcgttcag ttgcgcattg agcacggtga
     2701 aagaagtatc tgcctggaaa ggccgattgc ccgtcaccaa ctcgtaaagc gtaaccccaa
     2761 aggagtacag gtcggaacgc gcatcgaccg tgccgccgcg gacctgctcc ggtgacatat
     2821 agtagaccga ccccatggtt gtgccggggc gcgtaagctg caaatcgccg ccggatttcg
     2881 caattccgaa gtccatcaac ttggccagac catgcgcggt caccatgatg ttggaaggct
     2941 tgatgtcgcg atgcgtgatg ccgcggccat gcgcataact gagcgcggag agcacctgca
     3001 tcgcatactc gagtaccaga ttgagcggaa tccgcgactg gctggcgagt tgatcgagtg
     3061 tggaaccctc gacaaattcc atcaccatca ccaattggtt ctcaaactgg aatgcagttc
     3121 gcagctgggc gatattcgga tgttccagag tcgccagggt gcgaatttcc gccgtaaagc
     3181 gtgcggcgag ctccggctcc gatgcgtagt ccggcagaag gaccttcatc gcctccactc
     3241 ggtcggagat gacattgcgg acgcggtaga cgcgccccat cccgccggtt ccaagcaggc
     3301 tctgaacttc gtaatcacca atgcgctgac ccgcttctaa cgccatgtgc aaacctcgtg
     3361 ggacagatgt gtcgggggta tagggccaac aactgaggag cgcaacgagt ttactgtatt
     3421 tactactgca ttcggggaga aatctgttcg aaagcagcgt agtcggagga tcactcccgg
     3481 ggtattcttt ccatgcgcat ggcatgagtg gccggcggaa ttttgatcca gaaccgggac
     3541 gctgatgcgg tgcgaatgcg attcgcataa aatcgcggaa ccggttgtgg gaacctgcga
     3601 gtgctcagca atgtccgtac ggaatgccct caaaatcgac gcgttcggca tcagcgacgt
     3661 cggctgtcag cgcacgaata acgaagacag cttcagctat gacctggcgt cgcaactgtt
     3721 cgtggtgtgc gacggcatgg gcggcatggc ggccggtgag ttggccagcg gaaccgcggt
     3781 gcaggaattg ctccgcagct ttactgcttc ggaccccggt cagcccatcg aggagcgctt
     3841 atacgacgcc atcgcggacg caaatcgcag cgtcttcgcc ctggcggagg gaagtgccga
     3901 actgaaagga atgggcacca ccctggttgc agcctgtgtg gaaggcgaga agctggtggt
     3961 agccaacgta ggagacagcc gtgcgtggtt tctgcgcaat ggcagctgcg cgcagatcac
     4021 acatgaccac tccttcgtcg ctgaacaggt gcgcatcggc gcgatgagcc ccgagcaggc
     4081 cggcgtgtca ccgctgcagt cacttctgac ccgcgccatc ggacaggcag aggccgtcga
     4141 gcccgatatc ttcaccgccc cgctggagcc gggagacatt cttttgctgg ccaccgatgg
     4201 gctgtcgcgt tatgcggaca ccgatacaat tgcagggatt gttctgaaca gcgcaacgct
     4261 ggagcaggcg tgccaggcgc tcatcgaatg cgcaaaatcc cagggcgcgg tcgataacgt
     4321 gacgtgtctg ctgttgcagg tcatcgaagg cgaacccgag acctccgcgc cttagtgagc
     4381 gacgacggag acgcaacgaa ttccgctgag cccgcccggc atcagataat tcgcgtttgc
     4441 tcctgaaaac tccaggtgaa caacgagtgg cgttccgttc acgacaatgg gcgtgttcga
     4501 gacctctagc gggtaggcaa ggcgaacggg attgccggcc tgttccacgt gacccttgtc
     4561 gatcagatgg agcaacgccc acggtccgga aaactgcagc aaaggcaacg tacccgtcgc
     4621 ataggtggcg atcaactgag attgctggct cgtttgcggc gaccacgtga actgatgcga
     4681 aacgtcggat cctgaaattc gctgctggtc aatttgcagc gtcaccccct gaatccctct
     4741 tgaaggcagg atgtgcgcgt tgaacgtgat ccctccggga gtggcgggat agaggagtgc
     4801 ggaaagtccg gcgacgcggt tgaagaacct caggaaatcg gtattgagcc gcatcgggct
     4861 gttcggcgcg gcgacgaagg ttgtgccttc ctggatcatg agcggcttca gcgtcgtgtc
     4921 gtagaactgc cacagcgcgc cactacccgg ctgcagtacc ccggagagtt cagccggcgt
     4981 tgcgtcgacg gtcgagttgg gattgaatgg gaactttgtc atgagagggg cgaacgccga
     5041 gcagaagccg gcgcccgcgg cgttcgcctg ctgcggaccg cgtccacgca ccacctcgtc
     5101 gacagaattg atgggcgcct gcagcagcgc cagcaccgtt tgatccacat gtccctgcgg
     5161 atccagattg aacgcctgcg aagtctgact ggctgcggtg tgggcggagg ccgaggcggt
     5221 cagaatcggt gtgacggccg caggattggt cgctgccgtg ggatcctgcg cgacctgcgc
     5281 taccgcactc tgcagaccca gcagcccgtt gacgtagttc gtgttgcccg agccgacgaa
     5341 tttttccgcg ctttgcggcg cgaccaccgc ctgtaccggt tgaaattctt tcgcgatctg
     5401 cggatcggcc acagcggtgt tgtgcgatgc ggtgtagaaa agcgcaagca gcggcgaact
     5461 gggattcgaa acgatctgaa gctttgctgc cgcgtcgccg aggctgtgat agcggatcac
     5521 ctgcgccgca tgcagccacg cccgccactc ggactggaaa tcctgctgat aacgggtggt
     5581 cagctgctgc atgacgctgg cgcgatcgag cgacggcggc gcctgattgc caagcaccca
     5641 cgcctccccg ctgaagtagc gatcgggatg caggatcgcg tcgtgcatga agtcgaagcc
     5701 atcgtgcgtg aaggcgcccg ggatgatgcg cgcgtcgacg acggtggacc cggaaccggg
     5761 atagtcgcga ttgaagtcga tcggatgcgc gactttgccc gctgccgtga gcatctgttg
     5821 gtagatgcgc tcgaagccgc cgaagctcgc cagataggtg cgcgcgtgcg tgaccaccga
     5881 catatccgcg gcgatcgaat agggattttt gcgcgccagc tgtgcggcgt agaaatcgaa
     5941 ttgttggcgt gcgagccgga gctgatcgtc ggtctcaggc gtccggccgt tttcccagta
     6001 ctgcaccaga acgggactga gaaactccac cgtactctta tcgggattgc tggtggtgat
     6061 cagataggcc ttgagcggat tgtaggcagc ggaatagtca gcaccggcag gcggcgtacc
     6121 gggaagtcca ccgagcgcgc tcagcagact ggcctgcgtg ttggcgagca gcagggcgcg
     6181 gaatcggtcg aaataaatct ggcgcgccgg cttcaacaga tcgtcgccgc gatagagccc
     6241 ccagcgataa ctgagcggcg cgccgtcctg ctggtatttt tcaagctgaa cgatcttctg
     6301 gcgcagagcg tcgagggcag ccaggtctcg cgtgggcgcg agtgtgcccg ccggaacact
     6361 gccggaaggc agcgcactgg ccgccgaagc gatcgattgc acgaggctgg agttgttcgc
     6421 ccacgaaacc accagacaga tgaaccacac aaacagcacg aaagagaggg atgccagcgc
     6481 gatccggcgg aagatgtggg tgcgtccgct gttgctcgtt gccgccaggg ccgcccgatc
     6541 gtcgagaatc gctgacgaaa agaggcgcgg caggaagcac cactgcgcga ctttctgcga
     6601 aaccatctgc ggcgcggggt tcgctgcggc tgcgcgcatc tgctcaatgg aaaacatgcg
     6661 ggtggccccg gcgtctgcgg gtggaggtgg cgtggcagcc gcggaggcga caacctgctc
     6721 gatgacctgc gcccggaccc cgctgaaata gaagccgcgg agataggggt tggcgttcag
     6781 atggctgggg cgggccaatt cgaccagaaa cgtggtcagg gcattgcgca gcttgtttag
     6841 ctcgcgcgga aattcgtaaa ccggatccag attctttgca tcgttctcgc gcgtcaataa
     6901 ttccaggcgg aactcgccca gagagaagat gacctggtcg agtgaagtgg tgatttcctt
     6961 cgtcgcctgt tctgcataaa gcccggcgcc ggattcggaa cgctgcagcg ccatgcccac
     7021 tggctgcaaa gcctcatcgg gacttagatt gcgaacgtac tcggtgaagc tcggaacccg
     7081 gtcgagcttc gtaacgatga catagacggg gattcccgtg cccatctgcc gcgctaaatc
     7141 gcgcagcatc tgattcccac cccgtgcggc gttcatcacc gcatccggcg cggatgcgcc
     7201 caggaactgc tcggcactta cgcaaaaaac ggcggcccgg acaggggcct gcttgcccat
     7261 ggccgagcgc accagcttcg gccgtgtttt ccgaatgagc ttgctccaca gcgctggatt
     7321 ttttcttacc gcctcgcccg cttcaaccag aacgcattca cgcgtgtacc agacgttggc
     7381 gacagatgtc ggggcgatct cctgattgcg ataaacctgc ccggcgatca gctcgggatc
     7441 gagtccgcat ttcagcacag tcgtcgtctt ggcggagttg gcctcgccca gcacgtagag
     7501 cagcggaaga gtgtccaggg tcttcggccc ggtgcgctgc gcggtggcga gctgcctttc
     7561 cgcttcgcgc aggatcgtat ccagatcggt cccgccggga agtcctgcgg cggcgctgtc
     7621 acgtttcttc tcgcggaagt agagaaagag gatgatgccc gccacaccgg ccccgataac
     7681 aaggagcagg atgcgaagca ggatcagcgc ggtgccagtc agacccatga gaggtcccgc
     7741 cgcccaggcg agcaggatcg caacgatcag gactaaggct gaaaccagaa tcgatagcaa
     7801 cgcaggctcc tatggcgcgc tgaaggtgga actttgaacg tgggcaacac cggagcccag
     7861 gacaagatcg aatccgccga aggcaacgaa aacgagagcg gcgagtgcgc acacggcgat
     7921 gagcagagct cgggtccagg gatctttagt gcgcacagca cgcacttccg gacggacaac
     7981 gagaggcgtt aggtgcgccg gaccccgcac acgctggatc ttcgcaatcg cctgccgctg
     8041 gagctgatgc agctcgcccg agtcgcccag cgcatagcgt ccccgatagc ccacctgcat
     8101 acataagcaa tgaatctcga gagcgtcggc gttctccggc gagtccggct gcgaaagcag
     8161 cgtgcgcaaa ttgcgaaaaa atgtctcacc ggcaaggtgg ccaccgaaca attcttcctg
     8221 aagcggccgg cgagcccatt cagcaaagac cgggctttgc aggttgagca cgctctcatc
     8281 catgtaggcg acaacggcga agaccgccat ctgcacggtt tcgctggagt aaccgagtgc
     8341 gcgcccatcc tgcatcgccc cttgcagaga acggcgcata tgcgcgcgga agctttccgc
     8401 atcctgcacc tgctgccgct ggaagcgaac ccgcaggatg gcggtgagcg tttcctggaa
     8461 acacaaggcg agactgttga cgcggggaac gctcatgaag gctccacgac gacgatgacc
     8521 tcgaacaccg ggtgggaaat ctcacccgga atataaatgc ccacgctgcg cgtttgcaga
     8581 atgtgctccc agcacgggcc ggatgtttcg atggagaagt aatgcatgtc cgcctcggcg
     8641 cgaacggccg ccggcggaac cggcagatgg gtcagggtga ggccgggcag agctcgcctg
     8701 accagttcgg gcacgaattt ggaagagcag actttgacaa gcatcgggac ctggcgaagc
     8761 agatcggact cgcccataga agagcgaatc ccaacaatcc agcgggcccg ccgcagacag
     8821 cgctcatcgc gaacctcggc cgcctgaata taaggctcga cgggatgaaa atcgagtcga
     8881 acagtattcg aaggcacgac aatctcgagg tgtcgacgga tgtgcgcgtc cagcgcgcgg
     8941 aacgctggcc cggggtcgcg gtggtcgtac gagggcagtt ggcgggggtc ggaatcgatc
     9001 gaaaaagtac agagcgcacc cgcaaggcga gacaactcga ggaacacgtc ctccgggtgt
     9061 gcatggcgcg tggtcgccag atgctgcaac ggcgccagcg cgctgtgcag tgcgtgaaga
     9121 aaccagtagt tcgccacgtc cagcgccccc gtaccggctt caaagcgccc ctgccggcgc
     9181 gcgctgcgtg agacggttgc gcttttttcc ccgacagcgt ccagaaggcg cttcaccagc
     9241 agcataagcg gctcgctggc gttcagccgc aggcacggcg ggatgaaatc ctcgtcgtag
     9301 accaggtgtg cgcggccgtc gcgcagaacg cgcgcgaccg gaatagcgag catctccgga
     9361 gtcagctccg cttccgtcag aagacggatg ttcttgcggc ccagcgctac ttcgcgttcg
     9421 tccacgccat tggtttcgtc gcgcagcacg cgctgcatta cggaaaagcg ggtgccgttc
     9481 gcggcatcgc tcgggtcgaa gtcgaagcca ttgtcacgcc gcccggcaac agcgaggtag
     9541 agcggcagcg cggcatcggt ggcaggaaag agatctgcga gggtccgtgc cttcggtgcg
     9601 gggtcggaac cgggaatgtc gaaagtcaat ccatcgggaa agattccgga ggcgtaaaga
     9661 agcgccgcat ctccgttgcg gatcgctttc tgatccagtt ctgcgtgcag caggccccag
     9721 ggctcatgcc aaaggcttgc cgccagaaag gcgaccgaat cttcgaagta gcggctctgc
     9781 gtctgaaaat ggtgagggct gaggtacatc ccctccgacc acaccacgcg tgacaggaat
     9841 ctcatagatc aaaccggtac tggagtgcgg cagctgaacc gtgtgcagac agggtacagc
     9901 cccggcgatc agcgggtggg cgcttcgctg atgaccttct cactgagtgc cgccaggcgt
     9961 gtgtgaatcg taggctgccg gctctttact gtcaagcaaa cgccatgtac cctggtgctg
    10021 agcaaacgat cctggtgaag aggacggcga tgtatggatc agccgaagat tcccctgggg
    10081 aatccggagc ccggccagcg gaacgaaacc gagaattcag ggccggcgag tgcaacagga
    10141 atctttggaa ccatcgcgcc gtccaacccg tcgccggatg aggatctgtt tgcctccctc
    10201 gcccgcatga atccttcctc cgcgccggca accccgccat tggatacaga agacgctgtg
    10261 aaaccctcgg ttgaaccgga gaaacccgcg caatccccgc ggccgggtga gttcacgcag
    10321 atgctgcagt cgctgaaaaa gccggagtcg ggatcgtcgc cgcaacctgc cgcaaccccg
    10381 ccgcaggact tggcccgcat cttcaggcag atttccgtgg aacggaaacc tgcaccagaa
    10441 acgcctgtgt actcgcccaa accggcaggt tctccggcct ccccaccggc ggctctttcc
    10501 ccgtcggccg cagccgctcc gcaaccgccg gcagccggta caccaggaag tttcaccggc
    10561 atgtttcagt ctctcggcgg ccagcttgac gcaaatcagc ccgttcctgc acccgcggca
    10621 ccggcagcgg ccacacccgc gccatccgcc gcggaggcca ggcctgcttc gtcgccgcca
    10681 ggtgaattca cgcagttttt caaagcggtt ccgagtgccg ccgcgcctcc cgcccagcaa
    10741 ccgccccctg gctttccggc gcagccggtg caaaagagcc ctggggcgtt cacgcagatg
    10801 ttctcgcaac cggcgacacc ggctgcagca cccgacccgg cttttccaac gcagctggcg
    10861 cagaagaacg agccgggatc cttcacccag gcgttttcgc aaccgactgg tccagcggct
    10921 ccttcctcgc cacccgcata ttcctcccca cccgcatatc aggaggcgcc taagccgcaa
    10981 agcgattttt ggtcgggcgc aattcctccc gcgacccctt cgtccacccc tcccgggcag
    11041 ggcggattca cgcagctctt tcaatcgttg aatgacgaag gccccgcgcc ctataaacct
    11101 cccgaggcgc cgttttcacc tgcaccagca gcagctccag catccgccgc cgctccagga
    11161 ggcggattca cccagctgtt ccgtacgctg tc
//
LOCUS       DASZYN010000257         6323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1037345, whole genome shotgun sequence.
ACCESSION   DASZYN010000257 DASZYN010000000
VERSION     DASZYN010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6323
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037345"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VHE33_06700"
     CDS             <1..835
                     /locus_tag="VHE33_06700"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HVT97179.1"
                     /translation="KAKEKLQAVADKIGYPDKWRDYSKLEMKPDDALGNSLRSREFEV
                     DYQLNKIGKPVNRQEWQMTPPTVNAYYDPSMNDINFPAGILLPAFYDKDATDATNYGH
                     IGAVVGHELTHGFDDEGAKFDAKGNLKDWWTPADEKNFQQRTDCVANEYSQFNAVDDL
                     KLNGRLTLGENTADNGGLRLAYIALMTRLAEGKPSANTAEQKAESSLTPQQQFFLGYA
                     QNYCSNARPEFLRMIAQTDPHSPDPLRVRGVLVNMPEFAHAFKCKPGQPMAPVKSCRV
                     W"
     gene            1061..1246
                     /locus_tag="VHE33_06705"
     CDS             1061..1246
                     /locus_tag="VHE33_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97180.1"
                     /translation="MKKTFRVAVLSTVLALCAAPVFADPGGNDPPPPGQKGGSNGTLS
                     PGTTGSIILSILTTVAG"
     gene            1334..2101
                     /locus_tag="VHE33_06710"
     CDS             1334..2101
                     /locus_tag="VHE33_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97181.1"
                     /translation="MHFTALTIFYWIEAVVVDLALLFVLFYRHRWRQFPILTFWLIFL
                     VARSIALMVLYANDLRRGYTITWMACLALDFALQLGIVVEIARIVLRPTGTWVQDARS
                     RFAMGALAAFAVAGIFTWWISPPAANARQMWQVRANLFTSLVICELFIVMTMAANRMG
                     LGWRNHVMAIGQGLTAWSTIMVVKTSVESYFGTQHHYLQLEQIRYLTYPAAVCWIAVQ
                     LWRDEPERKPISSDLQEYILALHRRVEYDLRRLDARH"
     gene            2160..2702
                     /locus_tag="VHE33_06715"
     CDS             2160..2702
                     /locus_tag="VHE33_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97182.1"
                     /translation="MVPYLLLGAVVGCLIAAALKTQLSAIRVDRRSWQDLVAQLKRIE
                     FERVTSVARDYLDPHEGQISLEPSDMWLMLGGRDGLRRMRENAKLMVLLAAHAQQWNF
                     DEGVIVTERMRRDAVRLRSAIRHIELALTFHRILRRSATLIPFYLHEAASSYYLMRQR
                     LLALYQTSHSGLYPQLADVL"
     gene            2887..3243
                     /locus_tag="VHE33_06720"
     CDS             2887..3243
                     /locus_tag="VHE33_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97183.1"
                     /translation="MANRDKEKTEPEPKQPHHSVMMDAIRHVGPTLEAEGTFLPSDEL
                     PENVIEREGLIESGTRGAAAEDEYDREADAMGAERLQTRLPGDTSSDPHTDTVADNAA
                     TMDDDRKAKKKGRSAA"
     gene            complement(3283..5478)
                     /locus_tag="VHE33_06725"
     CDS             complement(3283..5478)
                     /locus_tag="VHE33_06725"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HVT97184.1"
                     /translation="MRILSHWLRSYVPALSVSDRTLADDLTLRGIAVEGVFDLGEFGS
                     LFDMDITTNRVDAMNHYGIAREMATIYGLELQALDPTVSTGRTIPGGITPGRSFPISI
                     EEPKLCGRFTARVLRDVKIVPSTGIVAERFRLLEQKPISNAVDATNYCTLAIGQPTHV
                     FDLDKLEGGIIVRRARKGEKLRTLDGVERTLDPDDLVVADEKKAVGLAGVMGGWDTMI
                     TAETKNILVEAAWFDPASVRRSSKRHLLHTDASHRFERGADFNAPPIASALVSRIVLE
                     SGGTAEGELVDVVIPEAVARTAKRPSVMFSLSEVHRILGPTEDPAGITAPMAVDILTR
                     LGCGVAENGHDGSAAQSSGRDLDPSRATIGTASITEGKTAHAGRRYSVTLPSWRLDLE
                     REIDLIEEIARVYGYNRFRNTLPAFAGSVVELPWAEKERAVRTTLLALGWNEAISSTF
                     CSAEDAARFAREPGSFVALGNPLSEEAGMLRPSLLPGMLDMLSLNISRDVEGAALFEM
                     GTAFSGGPDRVEERPTLAFGATGSAFVAQPASFYDLKGLAETLLGKFASRSLDFDRFP
                     AESGLMPGWLHPGRSARAVLDGATVGWFGQLHPAEAERRKLKQAVWIGEFWLDRLYKQ
                     ALRQPIIEELSRYQPVRRDFSFVLPDSVYWGRLASAIQALAIPELRSFEPKEVLRDAK
                     GKLAPIGHYSLLLRVLFQSHERTLREEEVQKWSQQVVAVLEQQGGKLRS"
     gene            complement(5509..5925)
                     /locus_tag="VHE33_06730"
     CDS             complement(5509..5925)
                     /locus_tag="VHE33_06730"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HVT97185.1"
                     /translation="MKQQVSASRAGQRVSGKSTPRTRYFRDLLVWQKSMQLAKAVYQG
                     TSGFPKSEVFGLSGQMRRAAVSVPSNIAEGHGRLSDGSMRVFLAQARGSLYELETQIE
                     LASGLGYLELEAEKELLESCREIGRMLNGLLAVLEG"
     gene            complement(5922..>6323)
                     /locus_tag="VHE33_06735"
     CDS             complement(5922..>6323)
                     /locus_tag="VHE33_06735"
                     /inference="COORDINATES: protein motif:HMM:NF013568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97186.1"
                     /translation="GLCVDTNITFADLKGTLDFAMKAFFGSSVKTRFFPSFFPFTEPS
                     ADVQISCPFCGGSGCRKCKHSGWIELLGCGMVDPAVFGFVQTKQPGYDPKKISGFAFG
                     MGIDRIAMMKYGISEIGLLFGGDVRFLEQFA"
BASE COUNT         1290 a         1936 c         1878 g         1219 t
ORIGIN      
        1 caaggcgaag gaaaagctgc aggcggtggc cgacaagatt gggtatccgg acaagtggcg
       61 cgactactcg aagctggaga tgaagccgga cgacgccctg gggaactcgc tgcgctcgcg
      121 cgaatttgag gtcgactacc agctcaacaa gatcggcaag ccggtcaacc gccaggagtg
      181 gcagatgacc ccgccgacgg tgaacgcata ctacgatccg tccatgaacg acatcaactt
      241 ccctgccggc attctgctgc ccgcgttcta cgacaaggac gcgaccgatg cgacgaacta
      301 tgggcacatc ggagccgtag tcgggcatga gctgacccac ggcttcgacg atgaaggcgc
      361 gaagttcgac gcaaaaggca atttgaagga ctggtggact ccggccgacg agaagaattt
      421 ccagcagcga accgattgcg tagccaacga atacagccag ttcaacgcgg tggacgatct
      481 gaagctgaac ggcaggctga ccctcggcga gaacacagcg gacaatggcg gtctccgtct
      541 cgcttatatt gccctcatga cgcggctggc cgagggaaaa ccctcagcca atacagccga
      601 acagaaggcc gagtcctcgc tcaccccaca gcagcagttc ttcctgggct atgcgcagaa
      661 ttactgcagc aacgcccgcc cggaatttct gcgcatgatt gcccagacgg atccgcattc
      721 tcccgatccg ctgcgggttc gcggcgtgct ggtcaacatg ccggaattcg cgcatgcctt
      781 caaatgcaaa ccgggacagc ccatggcgcc agtgaaaagc tgccgcgtct ggtaataact
      841 ctgataagtc gttttgatga gagttagacc cgatagtagt atcttcgcca gacttaagta
      901 actcttttgt tgagaccata agtagttgtt tcggtcgaga taaattagta ctttcgtgca
      961 gttttcggcc tgccaagtct ggaaatcttt gtgaattttg caattccact ttcgcgttag
     1021 tctcgggctg cgtcgatcga cgccacgagg aaaaagcccg atgaaaaaga cctttcgagt
     1081 tgccgttctg tccaccgtcc ttgctctttg cgccgctcct gtattcgccg atccgggcgg
     1141 caatgatccg ccgcctcccg gacagaaagg tggaagcaac gggactctgt cgccaggcac
     1201 caccggcagc attatcctgt ccattctgac cactgtcgcc ggctagaccg ccccgtcggc
     1261 ctgccgcgcc ccggggactt ctgccatacg cggaagtccc tggagtggcg tatcttaggg
     1321 tcaaggcgga ccgatgcatt tcacagcgct cacaattttc tactggatcg aggctgtggt
     1381 ggtcgatctg gcgctgctct ttgtgctttt ttatcgccac cgctggcggc aattccccat
     1441 ccttacattc tggctaatct tcctggtcgc tcggtccatc gccctgatgg tgctgtatgc
     1501 gaatgattta aggcgcgggt acaccattac atggatggcc tgtctggccc ttgatttcgc
     1561 gttgcagctg ggaatcgttg tggagattgc ccgcatcgtc ctgcgcccca ccggcacctg
     1621 ggttcaggac gcccggtcgc gctttgcgat gggggctctg gcagccttcg cggtggccgg
     1681 catcttcacg tggtggattt cgccgccagc cgccaatgcc cgccagatgt ggcaggtgcg
     1741 agccaacctg ttcacttccc tcgtgatctg tgagctcttc atcgtgatga cgatggcggc
     1801 caaccggatg ggcctgggat ggcgcaacca tgtgatggcg atcggccagg gactaactgc
     1861 ctggagtacg atcatggtcg tcaagaccag cgtggagagt tactttggaa cacagcacca
     1921 ctaccttcag ctggaacaga ttcgctatct gacctatcct gcagcagttt gctggattgc
     1981 cgtgcaactt tggcgggatg agcctgaacg taagccgatt tcctccgatt tacaggaata
     2041 tatccttgcg ctccacaggc gcgtcgagta cgatcttcgc agacttgacg ctcggcatta
     2101 gtagctgcgg cataattgga ttcggagccg ctcggctctc ctgaaagaaa ggctttttcg
     2161 tggtgcctta tctcctcctg ggtgcggttg taggttgcct gatcgcggct gccctcaaaa
     2221 cccagttatc cgccatccgg gtcgatcgtc gcagctggca ggacctggtg gctcagctga
     2281 agcgcatcga attcgagcgt gttacttccg tagcgcgcga ctatctggat ccgcacgaag
     2341 gacaaatttc ccttgagccc agcgacatgt ggctgatgct cggcggccgc gatggactcc
     2401 gccgcatgcg ggagaatgcc aaactcatgg tcctgcttgc cgcccacgcg cagcagtgga
     2461 acttcgacga gggcgtcatc gtcaccgagc ggatgcgccg cgatgccgtc cgtttgcgca
     2521 gcgccatccg ccacatcgag ctcgcgctga ccttccatcg gatcctgcgc cgctcggcga
     2581 ctctgatccc gttctacctg cacgaagccg cctcgtccta ctacctgatg cggcagcgcc
     2641 tgctggccct gtatcagaca agccactcag gactctatcc acagctcgcc gacgttcttt
     2701 aacgcggcgt ttctccaccc ctgtcatccc tgcttttccc caaaccccca ccctgtcatc
     2761 ccggccgaag cggagggatc tgcttctctt tcgcaaagag aaatttctcg gccaccctga
     2821 aaacaggacc cgtcagggac aacttcccgg cacccggggg cgctccgaat aagcagagga
     2881 gatgctatgg cgaatcgaga caaagagaag acggaaccag aaccgaagca accccatcac
     2941 tccgtgatga tggatgccat ccgccacgtg ggaccaacgc tcgaagccga aggaaccttc
     3001 ctgccgagcg acgagctgcc ggaaaacgtg atcgagcgcg agggtctgat cgaaagcggg
     3061 accaggggcg cggcggccga agatgagtac gatcgcgaag ccgacgcgat gggcgccgag
     3121 cgtctgcaaa cccgcctgcc cggagacaca agctcggatc cgcacaccga taccgtcgct
     3181 gacaacgccg ctacgatgga tgacgatcgc aaggcgaaaa agaaagggcg gagcgctgcg
     3241 taagcgagtg aagagttatg aagtgacaat gtagacgtgc gatcagctac gtagcttgcc
     3301 gccctgctgt tccagcacgg caacgacttg ctgcgaccat ttctgtactt cttcctcgcg
     3361 cagggttcgc tcgtgcgact ggaacaacac gcgcagcagc agggagtagt gaccgatcgg
     3421 cgcgagtttg cctttcgcgt cgcgcagaac ctccttcggc tcgaagctgc gcagttcggg
     3481 aatggccaga gcctggatgg ccgaggcgag ccgtccccag taaacgctgt ccggcaaaac
     3541 gaaagagaag tcgcggcgca ccggctggta gcgcgacaac tcctcaatga tcggctgacg
     3601 caatgcctgc ttgtagagcc gatcgagcca gaactcacca atccacaccg cctgcttgag
     3661 cttgcggcgt tcggcctcgg caggatgcaa ctggccgaac catcccaccg tggcgccgtc
     3721 gagaaccgca cgggcgctcc gcccaggatg cagccagccg ggcatcagcc cagactcagc
     3781 agggaagcga tcgaaatcga gtgaccttga agcgaacttc ccaagcaagg tttcagccag
     3841 acctttcagg tcgtagaagc ttgccggttg cgcgacaaaa gcgctgcccg tcgcgccgaa
     3901 ggccagggtg ggccgctctt ccacgcggtc ggggccgcca ctgaaggcgg tgcccatctc
     3961 gaacagggcc gccccttcga cgtcgcggct gatgttgagc gaaagcatgt cgagcatgcc
     4021 cggcaacagc gaagggcgca gcatgccggc ttcctcgctc agtggattgc cgagcgcgac
     4081 gaacgaaccc ggctcacgtg cgaatcgggc cgcatcttcg gccgaacaga aggtcgaaga
     4141 gatcgcctcg ttccagccca aagcaaggag tgtcgtgcgt acggcgcgct ccttttcggc
     4201 ccagggcagc tccacgacgc tgccggcgaa ggcgggcagt gtattcctga atcggttgta
     4261 gccgtagacg cgcgcgatct cttcgatgag gtcaatctca cgctcgaggt cgagacgcca
     4321 gctgggcagc gtgaccgagt aacggcgacc ggcgtgcgcc gttttgcctt ccgtaatcga
     4381 cgctgttccg attgtggcgc gagagggatc gagatcccgg cccgagcttt gtgcggcgct
     4441 tccgtcgtgg ccattttctg ctaccccgca gccaagccga gtcaggatgt ccacggccat
     4501 gggcgccgtg atccccgccg gatcttccgt ggggcccaga atgcggtgca cctcgctcag
     4561 gctgaacatt acgctcggcc gcttggccgt gcgcgcgacg gcttcaggaa tgacaacgtc
     4621 gaccagctcg ccttcggccg tccctcccga ttccagaacg attctgctga caagcgcgga
     4681 ggcaatgggc ggggcattga agtcggcgcc gcgttcaaag cgatgcgagg catcggtgtg
     4741 gagcagatgg cgcttcgaag aacgccgcac gctggcggga tcgaaccagg cggcttcgac
     4801 gaggatgttc ttcgtctccg cggtgatcat cgtgtcccat ccacccatca cgccggcaag
     4861 gccaaccgct ttcttttcgt cggcgacgac gaggtcgtcc ggatcgaggg tacgctccac
     4921 cccatccagc gtccgcagct tttcgccctt gcgcgcgcgc cgcacgatga tgccgccctc
     4981 gagcttgtcg agatcgaaca cgtgggtcgg ctggccgatg gcgagcgtgc agtagttggt
     5041 cgcgtcgacc gcgttggaga tcggcttctg ttcgagcaac cggaagcgct cggcgacgat
     5101 gccggtggac gggacgatct tcacatcgcg caggacgcgc gccgtgaagc ggccgcaaag
     5161 ctttggttct tcgatggaga tgggaaagga acggccaggg gtaatgccgc cagggatcgt
     5221 gcggccagtg gacacggtcg ggtccagggc ctgcagctcg aggccgtaga tcgtggccat
     5281 ctcgcgggcg atgccgtagt ggttcatcgc atcgacacgg ttggtggtga tgtccatgtc
     5341 gaagagtgag ccgaattcgc caagatcgaa gacgccttca acggcgatgc cgcggagggt
     5401 gaggtcgtcg gcaagggtgc gatcggaaac cgataaagcg ggcacgtaag agcggagcca
     5461 atgggagaga atgcgcatag cgagtcagcg agctagcgag tcagcgagtc agccttccag
     5521 gacggccaat agcccgttca gcattctgcc aatttcgcga cagctttcca ataattcctt
     5581 ttctgcttcc aactccagat accccagccc ggaagccagc tcaatctgtg tttccaattc
     5641 gtaaagcgag cctctcgctt gagcaagaaa aactcgcatg ctgccatccg agagtcttcc
     5701 gtgcccctct gcaatgttac tcggcacaga aacggcagct cgccgcattt gcccgctgag
     5761 tccgaacacc tcagatttcg gaaatcctga tgtaccctgg taaaccgcct tcgcaagctg
     5821 catcgatttc tgccacacca gcagatcgcg aaaataacgg gtgcgtgggg tcgatttccc
     5881 gctgactcgc tgaccggctc gcgaagcgct gacttgctgt ttcacgcaaa ttgctccaaa
     5941 aaccgcacgt ctccgccgaa gagcaggccg atctccgaga tgccgtactt catcatcgcg
     6001 atgcgatcga tgcccatccc gaaggcgaag ccgctgatct tcttcggatc gtagccaggc
     6061 tgcttcgtct gcacgaaccc aaagaccgca ggatcgacca tcccgcaacc gagcagctca
     6121 atccagcccg agtgcttgca cttgcggcat cccgatccgc cgcagaaggg acagctgatc
     6181 tgcacgtcgg cgctcggctc ggtaaacgga aagaacgacg gaaagaagcg cgtcttcacg
     6241 ctcgatccga agaaggcctt catcgcaaag tcgagcgtgc ccttcaggtc ggcgaaggtg
     6301 atgttcgtgt cgacgcagag gcc
//
LOCUS       DASZYN010000258         3703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_972154, whole genome shotgun sequence.
ACCESSION   DASZYN010000258 DASZYN010000000
VERSION     DASZYN010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972154"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..1694
                     /locus_tag="VHE33_06740"
     CDS             81..1694
                     /locus_tag="VHE33_06740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HVT97187.1"
                     /translation="MSIRTRRTLFSLVVFFAVCALGGMVLSQKVGAQSAQDESTFRDG
                     LKSFSQVYDVVAKNYAEPLTGDLPDKVIYDGAIPEMLHTLDPHSSFYDPKAYARMKED
                     QHGKYYGVGMLISPQQGKVVVVYPIEGSPAFRAGVHPGDVITSIDGTSAAGMSSDDVA
                     NRLKGPRGTRVNVTMTRVGASSPISFDLVRDEIPNNSIDLAYEIRPDVGYIHIRQFQE
                     TTGREFDDAVNNFGPNLKGLVLDLRGNPGGVLVEAVSVCDRLLKRGQIIVSQRGRAFP
                     EQVYRATHGNSDSFPIVVLVNRGTASAAEIVSGALQDHDRALIAGETTFGKGLVQTVY
                     TLSDNTGLALTTYHYYTPSGRLIQRNYSGVSLYDYYYNHDQKKDDKDREVKLTDSGRT
                     VYGGGGITPDVKVEAPKSNPFQELLLERDAFLGFTEDYVSHHPVAKDFTVDDAVINDF
                     KQYLSAQNYEYTPAELESNLDWVKMNVKSSIFGTEFGLTSQLKVKADWDPEITQAVNL
                     MPQAAQLEQHVQQVLAQKDAALTKGPGAR"
     gene            1891..3531
                     /locus_tag="VHE33_06745"
     CDS             1891..3531
                     /locus_tag="VHE33_06745"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVT97188.1"
                     /translation="MPMPAAHGPLDSQLDEYVAAYMPAMNAPGLTLGLTDTSATRRIA
                     CYGYADLERHLAVTADHRFQIGSITKSFVVLVILQLHDEGKIDLHKPVLDYLPGLPMV
                     TKFGPVTVHHLLTHTSGLPDNLAVFGPDPAERLVQGFAPGEHFHYCNAGFDILGLLAT
                     KIDGRAWRVLAQERILDRLGMTETRPVITTADRERQAVGYAPYWDDQVYARQGRLAPA
                     PNLVMDDTAGCIASTPADMATYARMLLHHGQGPKGRIVSEESFRLMSTPHIKAEEFSE
                     TASYGYGIAVDTLDGHKILRHTGGMVAFASSIHVDLDGGVAAFASINAMQGYRPTAVT
                     EYAVSLLRAEREAKSLPSAPAISDPRDVDNAADYAGSFTAADGGKLEFRADGKRLSLM
                     HGGTAVPLQHAGGDNFLSTVPGVMADHTIHFGRKEAQGKGDSKKQAPVVEVSYGPGWY
                     TASGYEGPHTFSVPEEYGFYTGRYQPDTAWAGAVAVYVRKGRLYADDDPLVPIGDHLF
                     RVGDESWIPITATFFHLFEGKARVLRVAGIDLHRVEVG"
BASE COUNT          701 a         1082 c         1220 g          700 t
ORIGIN      
        1 gggagcggct gctatactga tgggtaaagt cgcggtttct tcgccccaca tcggaggagc
       61 gcgccaggat tcctgacagc atgtccatcc gcacccgccg taccctcttc tccctcgtcg
      121 ttttctttgc cgtttgtgcg ctcggcggca tggttctcag ccagaaggtt ggcgcccagt
      181 ccgcacagga tgagtcgact ttccgcgacg gcctgaagtc gttcagccag gtctacgacg
      241 tcgtggcgaa gaactatgca gagccgctga ccggcgacct gcccgacaag gtcatttacg
      301 acggcgcgat cccggaaatg ctgcatacgc tcgatccgca ctcgagcttt tacgacccca
      361 aagcctatgc gcggatgaag gaagaccagc acggcaagta ctacggcgtg ggcatgctga
      421 tctcgccgca gcagggcaaa gtggtggtgg tgtatccgat cgaaggatcg cctgcgttcc
      481 gggccggagt gcatccgggc gacgtgatca cttcgatcga cggcacgtcg gctgcgggca
      541 tgagttccga cgatgtggcg aaccggctca aagggccgcg cggcacgcgc gtgaatgtga
      601 ccatgacgcg ggtcggggca tcgtcgccga tcagcttcga tctggtgcgc gacgagattc
      661 ccaacaacag catcgacctc gcgtatgaga ttcgaccgga cgtcggatac atccacatcc
      721 gccagttcca ggagacgacc ggacgggaat ttgacgacgc ggtgaacaac tttggcccga
      781 acctcaaagg cctggtgctg gatctgcgcg gcaatccggg cggcgtgctg gtggaagcgg
      841 tgagcgtttg cgatcgcctg ctgaagcgtg gccagatcat cgtttcgcag cgcgggcgtg
      901 cgtttccgga gcaggtttat cgcgcgaccc acgggaattc ggattcgttt ccgatcgtgg
      961 tgctggtgaa tcgcggaacg gcatcagcgg cggagattgt ctccggcgct ctgcaggatc
     1021 acgaccgtgc tctgattgcc ggcgagacga cgttcggcaa gggtctggtg cagaccgtct
     1081 acacgctttc cgataacacc ggcctggcgc tgacgacgta ccactactac acgcccagcg
     1141 ggcggctgat tcagcgcaac tacagcgggg tttcgctcta cgactactac tacaaccacg
     1201 atcagaagaa ggacgataag gaccgcgaag tcaagctgac ggactccgga cgcacggtgt
     1261 acggcggcgg gggcattacg cccgacgtga aggttgaggc gcccaagtcg aatccgttcc
     1321 aggaattgct gctggagcgc gacgcgttcc ttggtttcac cgaggattat gtttcgcatc
     1381 acccggtggc gaaagacttc acggtggacg atgcggtgat caacgacttc aagcagtacc
     1441 tctcggcgca gaactacgag tacacgccgg cggagctgga gtcgaacctg gactgggtga
     1501 agatgaacgt caagtcttcg atcttcggca ccgagttcgg gctgacgtcg cagctgaagg
     1561 tgaaagccga ctgggatcct gagatcacgc aggcggtgaa cctgatgccg caggcggcgc
     1621 agctggagca gcacgtgcag caggtgctgg cccagaaaga tgcggcgctc acgaaggggc
     1681 cgggcgcgcg gtaagttttc gatcagaaga agcatcgaac ggccggagca atccggccgt
     1741 ttcgttttgg cggactggga cttcctgctg gggtgctaga ctgctgccca cgccatgacc
     1801 cgaccttcat tttcacgtcg aacttttctc accacttcgg ccgccgccgc cggggtttcc
     1861 cttctgccga cagcgatgag ctgggcgcag atgccgatgc ctgctgctca tgggccgctc
     1921 gattcgcaac tggacgaata cgtcgctgcc tacatgccgg cgatgaatgc gccggggctc
     1981 acgctgggtc tcacggacac gtcggcgacg cggcggattg cgtgctacgg ctacgccgat
     2041 ctcgagcggc atcttgctgt gaccgctgat caccggtttc agattgggtc catcacgaaa
     2101 tcgtttgtgg tattggtgat tctgcaactg cacgatgagg gcaaaatcga cctgcacaag
     2161 cccgtgctcg actatctgcc tggcctgccg atggtgacta aattcgggcc ggtgacggtg
     2221 caccatctgc tcacgcacac ttccggacta ccggataatc tcgcggtgtt cggtccggat
     2281 ccggccgagc ggctggtaca gggttttgcg ccgggcgagc attttcacta ctgcaacgcc
     2341 ggcttcgaca ttctcggatt gctggcgacg aagatcgatg ggcgcgcgtg gcgggtactc
     2401 gcgcaggaac gaattctcga tcgtctcggc atgaccgaga cgcggccggt gatcacgacg
     2461 gccgatcgcg aacggcaagc ggtcggatac gcaccgtatt gggacgatca ggtttatgcg
     2521 cggcagggac ggctggcgcc ggcgccaaac ctggtgatgg acgataccgc cggctgtatc
     2581 gcatcgacgc ccgccgacat ggcgacgtat gcgcgcatgc tgctccatca cggacaggga
     2641 ccgaagggcc gcatcgtttc ggaggaaagc ttccggctga tgtcgacgcc gcacatcaag
     2701 gctgaggagt tcagcgagac cgcgagctac ggctatggca tcgcggtgga tacgctggac
     2761 gggcacaaga tcctgcgcca cacgggcggg atggtcgcct tcgcttcatc gattcatgtg
     2821 gaccttgatg gcggcgtggc ggcgtttgca tcgatcaacg caatgcaggg ctaccggccg
     2881 acggcggtga cggaatacgc ggtcagcctg ctgcgcgccg agcgtgaggc gaaaagcctt
     2941 ccctcggcgc cggcgatcag cgatccacgg gacgtggaca acgcggcgga ctacgcgggg
     3001 agcttcaccg cagcggatgg cggcaagctg gaattcaggg cagatgggaa gcgcctctcg
     3061 ctcatgcacg gcggaacggc ggttcccctg cagcacgccg gcggcgacaa ttttctctcg
     3121 acggttccgg gagtgatggc ggatcacacc atccatttcg gcaggaagga agcgcagggg
     3181 aagggcgaca gcaagaagca agctccggtc gtggaggtgt cgtacggacc cggctggtac
     3241 acggcttcgg gctatgaagg cccgcacaca ttttctgttc cggaggaata cgggttctac
     3301 accgggcgct accagcccga tacggcgtgg gcgggcgcag tggccgtgta tgtgcgcaag
     3361 ggccgactgt atgcggacga cgatccgctg gtgccgatcg gagatcatct ctttcgcgtc
     3421 ggcgatgagt cctggatccc catcacggcc accttcttcc atctctttga aggcaaagcg
     3481 cgcgtgctgc gcgtggcagg catcgatctt catcgggtcg aggttggctg atctcctcga
     3541 acggggcgca cggtaggctg tcactatgga gatacgattt gccgatgagt cggacgcacc
     3601 tgctttgatg gtgctggtca atcacgcctt cgaggtcgag gcatttttca tcaccggaga
     3661 gcggctcgac gcagcgcgca cgcgcgaatg cttcgagcgg ggc
//
LOCUS       DASZYN010000259         6524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_11446, whole genome shotgun sequence.
ACCESSION   DASZYN010000259 DASZYN010000000
VERSION     DASZYN010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11446"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VHE33_06750"
     CDS             complement(<1..428)
                     /locus_tag="VHE33_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97189.1"
                     /translation="MPRRNDITKILVIGSGPIVIGQSAEFDYSGTQACKALKAEGYEV
                     VLVNSNPATIMTDPELADRTYIEPLTKDYLDEVLRLESEMLKEEGRGGVFAVLPTVGG
                     QTALNLAVELSDSGILDKYGVELIGAKLEAIKKAEDRLLF"
     gene            complement(518..1639)
                     /gene="carA"
                     /locus_tag="VHE33_06755"
     CDS             complement(518..1639)
                     /gene="carA"
                     /locus_tag="VHE33_06755"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HVT97190.1"
                     /translation="MQAILALEDGRIFRGEGYGAQGECRGEVVFNTSLTGYQEIFTDP
                     SYAGQIVVLTNPQIGNYGTNPADNEAPKPFIEGLVTREFSPISSNWRSTQVADEYLER
                     FHVPVISEIDTRALVRHLRNNGVMRGVISTAESNPDVLVEKARSIRRMDGTDLASVVT
                     TKDRYEFEHDGPDPLKPNEKPATANLHVVAYDYGIKKNILRMLTNEGCRVTVVPAQTS
                     AEDVLALKPNGVFLSNGPGDPEPVTYAQDNIRRLAGRTPIFGICLGHQLVGLALGGKT
                     YKLKFGHHGGNHPVKRNDTGKVEITAHNHNFAVDPDSLNTSEVDLTHIDLNDNTLEGL
                     RHRTLPLFSVQYHPEASPGPHDSHYLFKDFRKMMEEWKG"
     gene            complement(1786..3951)
                     /locus_tag="VHE33_06760"
     CDS             complement(1786..3951)
                     /locus_tag="VHE33_06760"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HVT97191.1"
                     /translation="MFVSRSHSEKNSSFFAKLKSAAAISCLLAGTAPSYAQAPQQTAP
                     AAPGLPPEPTPNYTQPLFLHDTQRDFAKPRSHFPNPIAPYEPSTVQQPSFLNSQHLED
                     LIRDGKIYLSLGDAVLLALQNNYDIAIQRLNLDVADTDLLRTKAGQLPQGTPTSLVTN
                     TLGSSSAAVSSGGPGGASVAGTGVGGIAVSTSGAGPLPENLDPYLQGTIQLQRQKSPQ
                     LNTLFSGGKTSLTTNTDEYNFTYNQGFRTGTALQVGFQNNRVTSDSPFNVYSPSLTTI
                     FNAQLTQHLLQGFGWGLNSRFIVQATNNRRIADSVFRSQLLYTINQVEDIYWGLVSAY
                     EDVQSKQRAVDQSSQLEKDNEKQLQIGSMAPLDVVNARSATSSDQQALIASQSNLEYQ
                     QLLMKQAIARNLDDPRIASAPVIPTDRVTLAETPEERMSVEDLVRQAEANSPAVEQAI
                     LNMKNDEITLKAAKNGLLPTLDAYAFYGAQGLGGAQSPGCLNFFTGQTCDPGTYPTVG
                     YGTAVSHLFNSSGPNKGAGFTLNIPIRNRPAQALEARSQIEYRQAQMRLQQLYVQIRM
                     QITNDQYALTNDRASVNAAIANQEYNRQSLDAENKKLHLGASTTANVLSQQRSLAAAD
                     AQLISARARYATDRAALEQALASTLDRYGISIVDAAMGQVNTAPVIPGLEPAKNLPEV
                     TPPTQQQQLQQQEQNPTKPQAAPTQPQTTQPQSNPQPQS"
     gene            4211..5014
                     /gene="truA"
                     /locus_tag="VHE33_06765"
     CDS             4211..5014
                     /gene="truA"
                     /locus_tag="VHE33_06765"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HVT97192.1"
                     /translation="MATEKEGHVGSNWKVTLAYDGTDFSGWQVQPDRVTIQGTLADAV
                     ERVTGERVLPQGSGRTDAGVHARAQVASFWLEAAIPEENLQRALNRKLPEAVRVLRTE
                     RVTPEFHARHSARAKTYEYRIWRAEIAPPWEARYAWALNWPLDVQRMQEAAQKVVGTH
                     DFTSFAASDPDLAARRGLDDAERGENAGNVRTVFESDWRREGDLLIYRVRGDGFLHHM
                     VRNLVGTFVEVGRGAAEPAQIPRILEARSREEAGPTAPARGLWLDRVEY"
     gene            5057..6277
                     /locus_tag="VHE33_06770"
     CDS             5057..6277
                     /locus_tag="VHE33_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HVT97193.1"
                     /translation="MASPAPAASTVAQIAADRRVHQAFQWLHLQEQRILQWQSDLVAV
                     PAPPFGEKPRAEWLCERFRELELESPHLDEVGNAFAGRSGTRGGRKVLLSAHIDTVFP
                     AATPIEVHREGTRLSAPGACDNGAGVAAMLAIAAAMKQAGITTECDIVFVGNVGEEGE
                     GDLRGMRHVYRRDDGGVELSEIAAHIVLDGAGHESAVTHALGSQRHLVTITGPGGHSW
                     TDAGRPNPIVLLSRAIQRFSTVTLPDIPRTTMNVGTIEGGTAVNAIPERAAARFDLRS
                     TDPEQLIRLEVELHRAVEDAVLEANRGLRGEAGLVRFTIDKIGDRPAGRLPAGSELMA
                     LVEAVDRHLAIRTEMRLASTDANIPLSVGVPAVSIGGGGEGGGIHTRGEWYDAKGREL
                     GLKRILLLLLAAAG"
BASE COUNT         1151 a         1700 c         2346 g         1327 t
ORIGIN      
        1 ttaaacagga gccggtcttc ggcctttttg atggcttcga gcttcgcgcc gatcagctcg
       61 acgccatatt tgtcgaggat gcccgagtcg gaaagctcaa cggcgaggtt gagggcggtt
      121 tgtccgccga cggtgggcag cacagcgaaa acaccgccac ggccttcctc cttcagcatc
      181 tcggattcaa ggcggaggac ctcgtcgaga taatccttcg tcaggggctc gatataggtg
      241 cggtcggcca gctcgggatc agtcatgatc gttgccgggt tcgagttgac cagcacgact
      301 tcatagcctt cggccttgag ggctttgcag gcctgggtgc cggagtagtc gaactcggcg
      361 gactggccga tgacgatcgg gccggagccg atcacgagga ttttggtgat gtcgttgcgg
      421 cgtggcattc ttcttcgctg ctctcagtat gtcggtttca taaaaatggc gggaaatgaa
      481 tcccgccgat tggcaaatat cttctggaaa actcgattca tcccttccac tcctccatca
      541 ttttgcggaa gtccttgaag aggtagtggg agtcgtgggg gccggggctg gcttcggggt
      601 ggtactgcac gctgaagagc ggcagggtgc ggtggcggag gccttcgagg gtgttgtcgt
      661 tgaggtcgat gtgcgtcagg tcgacttcgc tggtgttgag cgagtcggga tcgacggcga
      721 agttgtggtt gtgcgccgtg atctcaacct tgccggtgtc gttgcgcttc accgggtgat
      781 tgccgccgtg atggccgaac ttgagcttgt aggttttgcc accgagggcg agtccaacga
      841 gctggtggcc gaggcaaatc ccaaaaatcg gcgtgcggcc ggcgaggcgg cggatattgt
      901 cctgcgcgta ggtcaccggc tcgggatcac caggcccgtt cgacaggaag acgccgttcg
      961 gtttcagcgc gagcacgtct tccgcagaag tttgcgcggg aacaacggtc acgcggcagc
     1021 cttcgttcgt cagcatgcgg aggatatttt tcttaatgcc gtagtcgtag gcgaccacgt
     1081 gaagatttgc ggtcgcgggt ttttcgttgg gcttcaacgg atcgggaccg tcatgctcga
     1141 actcgtaccg gtctttggtc gtgacgacgc tggccagatc ggtgccatcc atcctgcgaa
     1201 tcgaccgggc tttctcaacg agaacatccg gattcgattc cgcggtggag atgacgccgc
     1261 gcatcacgcc gttgttgcgg agatggcgca ccagcgcgcg cgtgtcgatt tccgagatga
     1321 ccgggacgtg gaagcgctcc agatattcgt cggcgacctg ggtggaacgc cagttggagc
     1381 tgatgggaga aaactcgcgg gtgacgaggc cttcgataaa gggctttggg gcctcgttgt
     1441 cggccggatt ggtgccgtaa ttgccgattt gcggattggt gaggacgaca atctgcccgg
     1501 cgtaggacgg atcggtgaag atctcctgat aaccggtcaa cgatgtattg aaaacgacct
     1561 ctccgcggca ttcaccctgg gctccgtagc cttcgcccct gaagatgcgc ccgtcttcga
     1621 gcgcaagaat tgcctgcatt gccgctccgt cgagtggatt cttctgattc tagcagggag
     1681 tggcgagggt atcgggcggc gccctgagca aaactggaca aaatttcggg agacgaggag
     1741 gcccgcgcga aggcgggcct ctccatgaac aacagccgtt tgcttttagc tttggggttg
     1801 cgggttggat tgcggctggg tcgtctgcgg ctgagtgggc gcagcctgcg gcttcgtcgg
     1861 gttctgctcc tgttgctgca gttgctgctg ctgggtgggc ggcgtcactt cgggcaaatt
     1921 ctttgcgggt tcaagacccg ggatgacggg cgcggtgttg acctggccca tagcggcatc
     1981 gacgatcgag atgccgtaac ggtccagggt gctggcgagg gcctgttcca gcgccgcgcg
     2041 gtcggtggcg tagcgagcgc gtgcggagat gagctgggca tcggcggcgg cgaggctgcg
     2101 ctgctgcgag agtacgttgg ccgttgtcga cgcgccgaga tggagcttct tgttttcggc
     2161 gtcgaggctt tggcggttgt actcctgatt ggcgatggcc gcgttcacgg acgcgcgatc
     2221 attggtcagc gcgtactgat cgttggtgat ctgcatgcga atctgcacgt aaagctgctg
     2281 cagacgcatt tgcgcctggc ggtattcgat ctgcgaacga gcttcgagag cctgcgcggg
     2341 acggttacgg atgggaatat tcagcgtgaa acctgcgcct ttgttgggtc cgctgctgtt
     2401 gaacagatgg gataccgcag taccgtaacc aacggtcgga taggttccgg gatcgcaggt
     2461 ctggccggtg aagaaattga gacaccctgg gctctgcgcg ccacccaggc cctgggcgcc
     2521 atagaacgcg taggcatcga gggtgggcag caacccattt ttggcagctt tgagggtgat
     2581 ttcgtcgttc ttcatgttca ggatggcctg ctcgacggcg gggctgttag cttcggcctg
     2641 gcggaccaga tcctcgaccg acatgcgctc ttccggcgtt tccgcgagtg tgacgcgatc
     2701 ggtgggaatc accggagcgc tggcgatacg aggatcgtcg aggttgcgcg cgatcgcctg
     2761 tttcatgagc agctgctggt actcgaggtt gctctgcgag gcgatgagtg cctgctgatc
     2821 gctggacgtc gcgctgcgtg cgttgacgac atcgaggggc gccatgctgc cgatctggag
     2881 ttgcttttcg ttgtccttct ccagctgact ggactgatca accgcgcgct gcttggactg
     2941 aacgtcttcg taagcgctga ccagacccca gtagatgtct tccacctgat tgatggtgta
     3001 aaggagctgg gagcggaaga cggaatcggc gatgcggcgg ttgttggtgg cctggacgat
     3061 gaagcgactg ttcagacccc agccgaatcc ctgcaacagg tgctgcgtga gttgggcgtt
     3121 gaagatggtc gtgagagagg gactgtaaac gttaaacgga ctatcgctgg taacgcggtt
     3181 gttctgaaag ccgacctgga gcgctgtgcc ggtgcggaag ccctggttgt aggtgaagtt
     3241 gtactcatcg gtgttggtgg tcaacgacgt tttgccgccg ctgaacagcg tgttgagctg
     3301 aggcgacttc tggcgctgca actggatcgt gccctggagg tatggatcga gattctccgg
     3361 gaggggtccg gcgccgctgg tggagacggc gatgccgcca acaccggtac cggccacact
     3421 ggcgccaccg gggccgcccg agctgacggc ggccgaactg ctgccgagcg tgttggtcac
     3481 caatgatgtg ggagtgccct gaggaagctg tccagctttg gtgcggagca gatcggtgtc
     3541 ggcaacgtcg aggttgaggc gctggatggc gatgtcgtag ttattctgga gcgccagcag
     3601 gacggcgtcg ccgaggctca gatagatttt gccgtccctg atgagatctt caaggtgctg
     3661 cgaattcagg aaactcggct gctgtaccgt cgagggctcg taaggcgcga tgggattagg
     3721 aaagtgactg cgtggctttg cgaagtcgcg ctgggtgtca tggagaaaaa ggggttgggt
     3781 gtagttcggc gtcggctcgg gcggaagacc gggagccgct ggggcggtct gttgaggcgc
     3841 ctgggcgtac gaaggcgcgg tgccggcgag caggcaggag atcgcagcgg cggatttcaa
     3901 cttcgcgaag aaacttgagt tcttttcaga atgactgcgg gaaacaaaca tgggccgtct
     3961 ccactcctct ggaagggcaa ctgcagttca atgaacgagg gcgcaaactg cgcttcgggt
     4021 gcgggcgaaa tgcgcgctcc aggccgcaaa aaaccgtttt ggcttctccc gaatacgagg
     4081 caagcctcca ccgggttccg taaactcgta aacaatcagg aaagctgagt atatcgtagc
     4141 gtttcgatgg ttcctggaga agcttcgttg cggaaaatcg tgcgacgaac aaggccgaaa
     4201 aagttaagaa atggcaactg aaaaagaggg acacgtcggt tcgaactgga aagtcacgct
     4261 ggcctacgac ggcaccgact tttccggctg gcaggtgcag ccggatcggg tgacgatcca
     4321 gggcacgctg gccgatgccg tcgagcgggt gacgggcgag cgtgtgttac cgcagggcag
     4381 cgggcgaacc gatgcgggcg tgcatgcgcg ggcgcaggtg gcgtcgttct ggctggaggc
     4441 cgcgatcccc gaggagaatt tgcagcgcgc gctgaaccgg aagctgccgg aggcggtgcg
     4501 cgtgcttcga accgagaggg tgacgccgga atttcacgcg cggcactcgg cgcgagcgaa
     4561 gacgtatgag tatcgaattt ggcgcgcgga gattgctccg ccgtgggagg cgcggtatgc
     4621 gtgggctttg aactggccgc tcgacgtgca aaggatgcag gaggcagcgc agaaggtcgt
     4681 cggcacgcac gacttcactt cgtttgcggc cagcgatccg gatttggcag cgcgacgggg
     4741 gctggacgat gcagagcgag gagagaatgc cggcaacgtg cgcactgtgt tcgagtcgga
     4801 ctggaggcgt gagggcgatt tgctgattta ccgcgtccgc ggcgatggat tcctgcacca
     4861 catggtgcgg aacctggtgg gaacgtttgt ggaggttggg cgcggagccg cggaacctgc
     4921 gcagattccg cggattctgg aggcgcgttc ccgcgaagaa gcgggtccga cggctccggc
     4981 gcgaggcttg tggctcgatc gcgtcgagta ttgagcgcca gcttccttct cgcccggtgc
     5041 tacgctgaaa tctcccatgg cctcgcctgc acccgccgct tcgactgtcg cgcagatcgc
     5101 ggcggatcgt cgcgtgcacc aggcgtttca gtggctgcac ctgcaggagc agcgcatcct
     5161 gcaatggcag tccgacctgg tggccgtgcc tgctccgccc tttggagaga agccgcgggc
     5221 ggagtggctg tgcgagcgtt ttcgagagct ggagctggag agtccgcacc tggacgaggt
     5281 gggaaacgcg ttcgctggac gatccggaac gcgcggtggg cggaaggttc tgttatcggc
     5341 tcacatcgac accgtttttc cggcagcgac cccgattgaa gttcatcggg aagggacgcg
     5401 gctgagcgcg ccgggagcgt gcgacaacgg agccggcgtg gcggcgatgc tggcgatcgc
     5461 tgcggcgatg aagcaggcgg gcatcacgac ggaatgtgac atcgtcttcg tcggcaatgt
     5521 gggtgaggag ggcgagggcg atctacgcgg gatgcggcat gtctaccgtc gggacgacgg
     5581 cggggtggag cttagcgaga ttgccgcgca tatcgtgctg gatggagcag gccacgaatc
     5641 ggcggtgacg cacgcgttgg ggagccagcg ccatctggtc acgatcaccg gaccgggcgg
     5701 gcattcgtgg accgacgccg gacggccgaa ccccatcgtg ctgctgagcc gggcgatcca
     5761 gaggttttct acggtgacgc tgccggacat cccgcgcacc acgatgaatg tggggacaat
     5821 cgagggcggg acggcggtca atgcgattcc ggagcgagcg gcggcgcggt tcgacttgcg
     5881 atcaacggat cctgagcagt tgatccggct ggaagtggag ttgcatcgcg cggtggaaga
     5941 tgcggtgctg gaggccaatc gcgggctgcg aggcgaggcg ggactggttc ggttcacgat
     6001 tgacaagatt ggcgaccggc ccgcggggcg gctcccggcc ggatcggaac tgatggcgct
     6061 ggtcgaggcg gtggatcggc acctggcgat tcggaccgag atgcggctgg cctcgacgga
     6121 tgcgaatatc ccgctgtccg taggcgtgcc ggcggtgagc atcggcggcg gcggcgaggg
     6181 cggcgggatt cacacgcgcg gggagtggta cgacgcgaag ggacgggagc tggggctgaa
     6241 gcgaattctc ttattgctgt tggccgcagc aggctgatcc agacgacaga attccacgga
     6301 ccgccaggtt gtttagactg cttctcgtga aagttctcgg cgttgtttgt ggaatggcga
     6361 tgacggcggc atcgattttt ggccagaccg gaccggccga tgcgatttat taccacggca
     6421 acattctgac tggagtcgac ctggaaggcg cgcagccgca gcgcgtgtcg gcaattgcgg
     6481 tcgcgcgggg cgagattgtt gccgccggag acgatgcgtc gatc
//
LOCUS       DASZYN010000260        17251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1231298, whole genome shotgun sequence.
ACCESSION   DASZYN010000260 DASZYN010000000
VERSION     DASZYN010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 17251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17251
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231298"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..593
                     /locus_tag="VHE33_06775"
     CDS             150..593
                     /locus_tag="VHE33_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97194.1"
                     /translation="MKVSHSGFSVELPDEWWVSAGMSGFAVSSLAYRYAPDAAGRRVC
                     LIRIVDIEPARRGPGVAVFNDDSEEGIAAKERVQRILRGFLENAELPPVELSKQADPI
                     PYPFRLNHGVHRLYCSLAAGYTHVPAVKFPDLGALDAGRDIEELC"
     gene            1023..3638
                     /locus_tag="VHE33_06780"
     CDS             1023..3638
                     /locus_tag="VHE33_06780"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HVT97195.1"
                     /translation="MTAQKAPVVTGDARVDELLSRMTLEEKLTLIHGTNEDPKVYQGQ
                     AGYLGGIPRLGIPGLRMADGPPGVLTRVPSQGETATMGVAATFSAKDAEENGVVIGRE
                     DRAAGIDVSLQPFVNIDRDLEFGRGYNTFGEDPYLSSVMGAEEIKGIQAQHVMAMVKH
                     YVGYDSPNENTYIDDQTLHEVYVAPFDAAVKAGVASIMCSYNKLNGTFACGNKDTLTT
                     ILRDQIGFRGYVTSDWGAVHAVDFINAGLDMEMPGTGIGIPSFFSPGPPPPPPPAHEM
                     DDMMAMFGGHIPEEPKPEEGGGGNWGVKLDPKRMSEALKDGTVNEAAITRAAGRVLYE
                     IVKFGYMDGMQKHNVTPQEVEANAKIIEKTGEDAAVLLKNEDHVLPLKAADLESVVLI
                     GPTAGQVDSIGINGERSVGLPWRQVGPLAAMKKVSGNPRIQFAVADDMTGTTVPASAL
                     SHDGKPGLMRTGGGAEKVDAELNFTKKKGNALPPNAKLVWKGTLTPPSAGDYWIYLQA
                     MGTNATISLDGKKLAQTGAFQGGVHGDILQANQDNVVPTTDAMDNVRRSVELTAGPHA
                     IEIETSPDSSNAPVQVRLNWYTPEQRKADHDAAIAAAKKAKTAVVFVWTRLTPVFGLP
                     GDQDKLVEEVAAANPNTVVVLNTSQPVALPWIDKVKGVVEMWWPGDEGGWSTANVLLG
                     KTSPAGRLPVTWAKALTDYAATDPRHPERSKKGVDGKTTYSEGVNVGYRWFDQERIEP
                     LFPFGFGLSYTTFEYSGLKTAEAADGGLDVTVQIKNTGGVDSDEVPQVYLGAPARIPD
                     GVQFPVRALVAFERVHVPAGGIKSVTMHVPIRQLQYWSTKEQKWVTARGKRTLSVGSS
                     SRSLPLQEVVDAEKP"
     gene            4085..7708
                     /locus_tag="VHE33_06785"
     CDS             4085..7708
                     /locus_tag="VHE33_06785"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HVT97196.1"
                     /translation="MRQVFGRPVYLMVFMMLCLGFAGAAYAQQNSEITGIVTDQTGAV
                     ISGAQITITDTAKGTSSTTVSNDSGYFSVPGLNPSTYELRASAKGFQSYVASGLVLNV
                     STTLRSDVKLQVGAEATTVTVQANALQVQTDENVVSTTISSLQVEKIATANRNFAALA
                     AMGLGVSSNLPDNNTPMAGAGGSSFSISANGLRQSHNIWLIDGGESDDRGGAGGIALM
                     PPQDAIAEFTMLTSNYPPDYGISSGATFSLSLKSGTRDFHGTGYEFNRNTAYDANDFF
                     NKYNGAHSPRQVLNYNVYGFNIGGPVWIPGLYNANRNKTFFFVNEEWRNIKAGSSTNK
                     NKTIATADIPKAGQDLVYVNPRHATGNAGIVVPNVGDPAFNAKLTAAGLTPGQPFPGN
                     KIPASLFDPNAVAYLNAGLFPAPSTALDESVETVNAPIKVRDDIVRVDHHINDKWALM
                     AHYMHENDNAHNAKPFLGWDGDTYNTVQSSEVTPSNSAAIKISGSISPTLLVEASMNY
                     DGNIIDIVNSANSALSTLTSKGWNAGQYFNNGRNEIPNVALGAPYGTNVEMGSAPWHN
                     AAEDYEPKVDVSYTSGKHSFKFGFSYNRYTKNQQLFGDANGYFHFNGTNGHLYSSNGD
                     QAACQGSTPAANCVQGDAMVDMLLGLAGSYDQQRAQPIRHYVNQTPSVYAMDTWHPTP
                     RLSLQLGFRYDALPQAWERNNYIANFNPAHFSPTATPAWRSDGSMDPTGPGFATFTVD
                     GATAPAYVNGIDLAGQAGVPRALVSNNYNTWQPRIGFSDDLFGNGKTVLRGGFGTFFE
                     RLQGNLIYNSATVSPFSYGPSANQVYLSDPHTSIQSGATATTPFFPGGQYEMAQHFPD
                     PAVAMYSLGLQRELSPSVVWVVQYVGNIAWHQEEYRHDNNFPLTTPMAVRAKGGSLGS
                     YTDAHNGATITLPTAGQGPTANNPNSFRTYQGLGDITAMETNTNGSYNGLQTGLRVQN
                     RWGLSGELDYTYSHEIDIQNADNACCVSNPWNLKYDKGAGNYDRRHILQANYVYALPI
                     FNHSQGLVKSIAGGWEISGTFIDESGTPPTNSTGIGISGAAGDTVGLGGGYTNRANVS
                     GKVHYTKKVTGWFNPAQFSDPTPAWLGGPNNGFGNSHKDAVVGPGRVNFTTSLYKSFA
                     MTERAHIELRFESFNTFNHAEFQNLNTGYTRPNSSGSYGNFSQVNNDWGPRLLELGGK
                     IVF"
     gene            7881..9056
                     /locus_tag="VHE33_06790"
     CDS             7881..9056
                     /locus_tag="VHE33_06790"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT97197.1"
                     /translation="MKAVVFAGLWGALVAAGWGQRTPAMDAQKQAALNLEHENRIPEA
                     EAAWRAYSHAHPGDPEPWAQMGLLEARQEHYARAIPLYQKALQLSPHVPGLRLNLGLA
                     LFKDGRSKEAIAEFKTLLKTAPPASPEAQRLRLLIGMAYYGMAEYTQAIPYLKRAAAQ
                     DKENLPLRLALAHSCLWSKEYPCVMETYREILTLNAESAEADMLAGEALDAMRDDTGA
                     LAQFRAAEKANPKEPYVHFAVAYVLMAQKRYADAVPEFQADLEADPRHAQARVYLADC
                     YVHLEDNAHAQPELERALKDDPSIALGHLDLGIIYAAQGRNQEALAQYLTAIRLNPQD
                     ADPHWRLSRLYQAMGETEKARAEAVLVSTMKKQAYQNLYQQISGPANHAAEVANPPQ"
     gene            9257..12709
                     /locus_tag="VHE33_06795"
     CDS             9257..12709
                     /locus_tag="VHE33_06795"
                     /inference="COORDINATES: protein motif:HMM:NF020768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0031221 - arabinan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinofuranosidase catalytic domain-containing
                     protein"
                     /protein_id="HVT97198.1"
                     /translation="MNAKVNSGLMFAALVLIGATTAMAQQNGTGASGASSRPQGPCDI
                     YAAAGDPCVAAHSTTRALYASYNGPLYQVKRQSDGKTLDIGVVQPSASPVPDAGGYAN
                     AAAQDAFCANTYCWITTIYDQSPKHNDLTQAPRGGFSGPAMGGFNNVPIADMAPVTIM
                     GHKVYGVFIEPGMGLRNDDPKGTAVDDQAEGQYWVINGRHYNSGCCFDYGNAEIDSRD
                     DDNGTMETAYFGDALPWFHGNGSGPWIMTDQENNLVGCVNADGSKHCENVPSIEWRFV
                     TAIAKGEPHHWTSMGGDAQQGDLSVMFSGARVNATYDPMRKQGAILLGNGGDNSNGSQ
                     GTFYEGAMTASGTFPTDATDQKVQANIVAAHYGVAPLQVSPAKAGDMPPGLQTFSPGS
                     SQETTVTFTNTTGAPATNVELSLTAPNGWKAVVSGTSEKSKTFAGPVTPGGNVSATFT
                     VTAGNAGFNGDLVGKARWMNGANSREQTETIAEKVRNASPVKINEFRISSGPPANGTD
                     SFIELYNAGSSSVDLSRWTLTEHPTQQAVFSTVTIPAGTKLAAHGFYLLGLSNSGLAT
                     AARAGEKTIYVRSVTGMKAGDTVSIGSGSSAETRKIAEAGTAASNMTTLWQPLPDGLI
                     TLPAGSTNVPVTSAAGFVVGQKIALGYGATYPTVPGEVEKYEVATVTAVGKPGTQAYL
                     ATDAAAGATNIQVTSAEDISAGDKIRLDIDSEGHGIETVTVTRVGTQADHSNLAANAS
                     AGATTIKVRSVRGFTVGGKLTIGTPTNRQTVTIASVGTGGPNGTGIEFSPALEQAHIN
                     DETVVQPGTGLELAAPLKFNHAANLPFSDRGTGITFEPATAFAHMSNEPVQALGTGIT
                     LDSPLAHDHAIDAVVRDATVKTAGYQGNPAPNQWFGGPELAANVPFFNFTITRREGSM
                     MLRDAAGVVVDSLNYGGLVDPWLGEGYQAESGAQHNGCYVTAPGWAGAVWPPMPTAGG
                     SNTSAGRLPDGADSDSNCNDFVTSPATTLGVASTTGATNIKVAGVEGFAAGQTIRIVE
                     GADAETVEIATVGTPGATTATAATEAGATVIHVGNPIGFENDETITIGSGDDAETAEV
                     VRRTRESTIVVTAPLARAHAAGTAVSGSGITLRAPLRREHAAEAQVTGSISTPGAPNQ
                     YFRK"
     gene            13145..16603
                     /locus_tag="VHE33_06800"
     CDS             13145..16603
                     /locus_tag="VHE33_06800"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVT97199.1"
                     /translation="MGESIRQCWNRLWLLTVPLILLGVAGFSSPLAIGQQTNANVNGV
                     VKDPSGAAIPNARVELTNVNTGVVRTTTTNNDGAYNFPSVVPGVYTMQASATGFAAVS
                     QPAVTLQVSQIATFDFELKVGSTTSSITVTATAAALQTSSSELGTVVTTNQVNDLPLN
                     GRNFTQLLTITTGVANVNTDQNGNGGGGWNGHTVGTTDIPAVNGAANRSNVFLLDGAN
                     DLNTLSGVYNYSPIVDAIQEFKTQGHNDLAEYGGAAGGLVTVVTKSGTNQYHGALWEF
                     LRNEQMDSRGYFESSRAPLRQNQYGASAGGPLSIPKLYNGKNRTFFYAAWESYRYASA
                     QETGALGPTAAMRAGDFSALGVPIYDPATTTLDPVSGQYTRQTFTQEYNEGPGNPSLC
                     GGDTNCIPSSRFNPITSLYAGLIPNSGALVNGSNVFVTGRSFTNQHSGTLRVDQNFGN
                     NDQLMFRYSQFDQYGQSPSGTIGSGFDSVVGHNYIGHWTHTYSPTTFSDVYFGRNYGY
                     SLIGTSWAGEDAAFLGQLQSLGMSSAWMTLDNKTYAPQLAADSYVGLTGSQLQGSGLA
                     DDWQFGGSFSKILGKHTLKAGVDIETNNFTSPIAYSGVYFGVKQTAGVGAEQGVGGNS
                     WASLLLGVPREANYRNIDEVAKGGWMDAFYVQDQFKATSRLTLNLGFRNDMVFTPIYG
                     TGKGGNYYTGNADPVTGQYILNARPPDCSATQGAPCIPNGIYTASSTPAPGGLPAHTI
                     VNPSLRVIQNSLADWGLRLGAAYRLNDKTTIRGGYGREYDEWATIVQLSQNFGGNWPA
                     VNTLDNAGLNTNVPTATAADPLQLGGGGAIVYPINDFSQVSQWMVDPNFKTPYYDQYN
                     AGIERQLPGNLALDANYVGATGSREDWGPTMNTPQPGPGDVQSRRPYPYMQQQWFDQS
                     VGHSQYNALQVTLNERNLHGVGFLVAYTLGHSNSDGCGLGASCDSSNPYHRSVDYGTS
                     DLNQKNIFSAAFTAQSPFTHSPDKVVSGLAGGWSMNGIVQFSSGQVYTVTTGSDPENI
                     GCCLQERVNVTGDPNSGSGLHTPAKWFNTSAFTQPAAYTYGTEKVNPFTAQMRHEVDL
                     SLFRQFHIGLGEERYFEFRADSFNLFNNVVFDRPDATISDTTFGQVTRQQNQPRQLQV
                     ALKFYY"
     gene            16710..>17251
                     /locus_tag="VHE33_06805"
     CDS             16710..>17251
                     /locus_tag="VHE33_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HVT97200.1"
                     /translation="MRSALTLLFSAAVLCGFLPAQSIAAPPIRFEDIAERAGVHFVVE
                     NSPTPEKHQPETMPAGVALFDYDGDGLLDIYLVNGAEMPSLVKTGPKYYNRLFHNNGN
                     GTFTDVTERAGVAGAGYGMGAAAGDYDNDGRDDLFVANVNGNQLFHNNGDGTFTDVTV
                     KAGLSGAVHNGRKMWSIAAG"
BASE COUNT         3668 a         4765 c         5662 g         3156 t
ORIGIN      
        1 cggagtgccg caatacgcgg cgccgccgaa tgtagcgcaa agccgtcact cggcagtgag
       61 taccattgtt gtagccactc tgtttagaaa aagcttcgga aatcgcgatt gtcgttgggt
      121 taagcgcagg ctaacctccc cacggcacca tgaaagtctc ccatagcggc ttcagcgttg
      181 aattgccgga tgaatggtgg gtctcagcag gcatgtcggg gtttgccgtg tcatccctgg
      241 cctatcgcta tgcgcccgat gccgcaggaa ggcgcgtttg tctcatacgg atcgttgata
      301 tcgaaccggc ccgtcgcggg ccgggagttg cggtattcaa cgacgatagt gaggaaggaa
      361 tagccgcgaa agagagggtt caacggatcc tgcgagggtt tctggagaat gcggaactac
      421 cgcctgtcga gttgtcgaag caggccgacc caataccgta tccgtttcgt ctgaaccacg
      481 gagtccacag actgtattgt tcactggccg ccggttacac ccatgttccg gcggtgaagt
      541 ttcctgattt aggggcgctc gacgctggcc gcgacatcga agaactctgc taagtcaatt
      601 tcctgcgatt gcgcctacca gcgtcaccaa cccactggct attgatacgg accatccgta
      661 gcgtcgcagg ttcatccggg ctttccgtta gcaatccaca cccggagccc tccgcgctgg
      721 tgaggagcat actctctggc ggcgtcggcg gcgcttcggt caggatgacc ctgcgtgttc
      781 agatcgtgac tggatcgcgg cgtcttggag atccgcatta cggtcgtttc ggcgcattgc
      841 gtcgcggttg ttgaacggaa tcgattgtgc tgtagactgt cgggcggttt gcaggatcga
      901 aacggccgac gtctgatcgc tttcttcgat gggcgtgttt cgaaaatcgg ggggacacca
      961 tgaaccggta cggaatgcgg tggttggcat tttgtggagc agcgttactc cttacgagct
     1021 ggatgacggc gcagaaggcg ccggtggtca cgggcgacgc gcgtgtggat gagcttctga
     1081 gcaggatgac gctcgaagaa aagctcacgc tgattcacgg cacgaatgag gatcccaagg
     1141 tgtaccaagg gcaggcggga tatctggggg gaattccgcg gctggggatt cccgggctgc
     1201 gcatggcgga cgggcctccg ggcgtgctga cgcgggtgcc gagccagggc gagacggcga
     1261 cgatgggggt ggccgcgacc ttcagcgcga aggacgcgga agagaacggc gtggtgatcg
     1321 ggcgggagga tcgcgcggcg gggatcgatg tgagtttgca gccgtttgtg aacatcgatc
     1381 gcgatctgga gttcgggcgc gggtacaaca cgtttggcga agatccttat ttatcgagtg
     1441 tgatgggggc ggaggagatt aaggggatcc aggcgcagca tgtgatggcg atggtgaagc
     1501 actacgtggg gtacgactcg ccgaacgaga acacctatat cgatgaccag acgctgcacg
     1561 aggtgtatgt ggcaccgttc gatgcggcgg tgaaggcggg cgtggcgtcg attatgtgct
     1621 cgtacaacaa gctgaacgga acgtttgcat gcgggaacaa ggacacgctc acgacgattt
     1681 tgcgggatca gatcgggttc aggggatatg tgacgtcgga ctggggcgca gtgcacgcgg
     1741 tggacttcat caacgcgggg ctggacatgg aaatgccggg cacggggatc gggattccgt
     1801 cgttcttcag cccggggcca ccgccaccgc caccgccggc acacgagatg gacgacatga
     1861 tggcgatgtt tggggggcat attccggagg agccgaagcc ggaagaggga gggggcggga
     1921 actggggcgt gaagctcgat ccgaagcgga tgagcgaggc gctcaaggat ggcacggtga
     1981 atgaggcagc gattacgcgg gctgcggggc gtgtgctcta cgagattgtg aagttcggct
     2041 acatggatgg aatgcagaaa cacaacgtga cgccccagga ggtcgaggcg aacgcgaaga
     2101 tcatcgagaa gacgggcgaa gatgccgcgg tgctgctgaa gaatgaagat cacgtgcttc
     2161 cgctcaaggc ggcggatctg gaatcggtcg tgctgattgg gccgacggcg ggacaggtgg
     2221 attcaatcgg tatcaacgga gagcggagcg ttgggctgcc gtggcggcag gtgggaccgc
     2281 tggcggcgat gaagaaggtt tcgggaaatc cgcggattca atttgcggtg gccgatgaca
     2341 tgaccgggac gaccgttccg gcaagcgcgc taagccacga tggcaagccg ggtttgatgc
     2401 ggacgggtgg cggcgcagag aaagtggatg ccgaactgaa cttcacgaag aagaaaggga
     2461 atgcgctgcc gccgaacgcg aagttggttt ggaagggaac gctgacgcct ccgagtgcgg
     2521 gggactactg gatctatctg caggcgatgg gcaccaacgc gacgatttct ctggatggga
     2581 agaagctggc gcagacgggc gcgttccagg gcggggtgca cggcgacatt ctgcaggcca
     2641 accaggacaa tgtcgtgccg acaacggacg ctatggacaa cgtgaggcgc tcggtggaac
     2701 tgacggcggg tccgcatgcg attgagattg agacgtcgcc ggattcttcg aacgcaccgg
     2761 tgcaggtgcg gctgaactgg tatacgccgg agcagcgcaa ggcggatcac gacgcggcga
     2821 ttgcggcggc aaagaaggca aagacggcgg tggtgtttgt gtggacgcgg ctgacgccgg
     2881 tgtttggact tccgggcgat caggacaagc tggtggagga ggtggcggcg gcgaatccga
     2941 acacggtggt ggtgctgaac acgagccagc cggtggcctt gccgtggatc gacaaggtga
     3001 aaggcgtggt ggagatgtgg tggccgggcg atgagggcgg gtggtcgact gcgaatgtgc
     3061 tgctgggaaa gacgagtcca gcggggcggc tgccggtgac gtgggcgaag gcgctgacgg
     3121 attatgcggc gacggatccg cggcatccgg agcggagcaa aaagggcgtg gatggcaaga
     3181 cgacgtacag cgagggcgtg aacgtcgggt atcgctggtt cgatcaggag aggatcgagc
     3241 cgctgttccc gtttggattc ggtctgagct acacgacctt cgagtattcg ggattgaaga
     3301 cggcggaggc ggcggacggc gggctggatg tgacggtgca gatcaaaaac accggcggtg
     3361 tggattcgga tgaagtgccg caggtgtatc tgggcgcgcc ggcaaggatt cccgacgggg
     3421 tgcagtttcc ggtgcgggcc ctggtggcgt tcgaacgggt gcatgtgccg gcgggtggga
     3481 tcaaaagtgt aaccatgcat gtgccaattc ggcagctgca gtactggtcg acgaaagagc
     3541 agaaatgggt gacggcgcgc gggaagagga cgctgagcgt gggaagttcg tcgcgatcgc
     3601 tgccattaca ggaagtcgtc gacgcagaaa agccgtgatt tcgtctacca agtactggat
     3661 cgctgtacgc gcttacaggc agatttgtcc ggaatttgga attttgcacg caaacatggc
     3721 acgcgccgga gggcagtttt ggaacccccg ttgtgctgga aacggttcgc tgtccgtgag
     3781 ttaggaaggg tcccgaacta tatgatccga aaagcggatc acggcgatcc aaattttgga
     3841 tttcaacccc gcggaggagc ttttcgccct cgtgacacga ggtagttacc gaagggaaac
     3901 gcgaggcggc ggtgtttaat aaaacgcttg acaacagtag aaaccgattg ttaggcttcg
     3961 ggaccgatcc caaaatagca gtaaaaaatg cagctgttca ggagagctgc gggatcaggc
     4021 gcagtttcga tacgcacgag aatgcgtagc aacgttggaa cgcaacttca tgaggaggca
     4081 attcatgaga caggtattcg gaagaccggt gtacttaatg gtcttcatga tgctatgcct
     4141 cgggttcgcg ggtgcagctt atgcgcagca gaactctgag attaccggca ttgtcacgga
     4201 tcaaacgggc gcagtgattt caggcgctca aatcacaatc acagatacgg caaaagggac
     4261 atcgtccacg acggtgtcga acgattccgg atatttctcg gttccgggtc tgaaccccag
     4321 cacgtatgaa ctcagggcgt cggcgaaggg tttccagagc tacgtagcca gcggccttgt
     4381 gctgaacgtt tcgacgacac ttcgttccga cgtaaagctg caggtcggcg ccgaggcgac
     4441 gacggtgacg gttcaggcga acgctctgca ggtgcagaca gatgagaacg tggtgagcac
     4501 gacgatctcc tcgctgcagg tggagaagat cgcgacagcg aaccggaact tcgcagcgct
     4561 tgcggcgatg ggtctgggcg tgagctcgaa ccttccggac aacaacacgc cgatggcggg
     4621 agccggcggc agcagcttca gcatcagcgc gaacggcctt cggcagagcc acaatatctg
     4681 gctgatcgac ggcggcgagt cggatgaccg cggcggcgcg ggcggcattg ctttgatgcc
     4741 tcctcaggac gctatcgccg agttcacgat gctgacctcg aactatccac cggactatgg
     4801 catctcgtcg ggcgcaacgt tcagcctttc gctgaagagc ggcacgcgcg acttccatgg
     4861 aacgggctac gagttcaacc gcaacacggc ttacgatgcg aacgacttct tcaacaagta
     4921 caacggcgcg cacagtccgc ggcaggtgct gaactacaac gtatacgggt tcaacatcgg
     4981 cgggccggtc tggattccgg ggctgtacaa cgcgaaccgg aacaagacgt tcttcttcgt
     5041 gaacgaagaa tggcgcaaca ttaaggccgg ttcttcgacc aacaaaaaca agaccattgc
     5101 gaccgcggac attccgaagg cggggcagga tctggtttat gtgaatcccc ggcatgccac
     5161 cggcaacgcg ggcatcgtgg ttccgaacgt cggcgatccg gcattcaacg ccaagctgac
     5221 ggcggcgggc ctcacaccgg gtcagccatt tcccggcaac aagatcccgg catcgttgtt
     5281 cgatccgaac gcggtggctt atctgaacgc cggtctgttc ccggcgccca gcacggcgct
     5341 cgacgagtcg gttgaaaccg tcaacgcgcc gatcaaggtg cgtgacgata tcgtgcgcgt
     5401 ggaccatcac atcaacgaca agtgggcgct gatggcgcac tacatgcacg agaacgacaa
     5461 cgcgcacaac gccaaaccgt ttttgggttg ggatggcgat acctacaaca cggtgcagag
     5521 ctcagaggtg acaccgtcca acagcgccgc catcaagatc agcggcagca tctcgcccac
     5581 gttgctggtg gaagcgagca tgaactatga cggtaacatc atcgacatcg tcaacagcgc
     5641 caactcggct ctgtcgacgc tgacgtcgaa gggctggaat gcgggacagt acttcaacaa
     5701 cggcagaaac gaaattccga acgtcgcgct gggcgcgccg tacgggacga acgtagaaat
     5761 gggatcggcg ccgtggcata acgcagctga ggactacgag cccaaggtgg atgtgtcgta
     5821 cacctcggga aagcactcgt tcaagttcgg tttcagctac aaccggtaca ccaagaacca
     5881 gcagctgttt ggcgatgcga acggctactt ccacttcaac ggcaccaacg gacatctgta
     5941 ttccagcaac ggcgatcagg cagcctgcca gggaagcact ccggcagcta actgcgtgca
     6001 gggcgatgca atggtggaca tgctgctcgg gctggcggga agttacgacc agcagcgcgc
     6061 gcagccgatc cgtcactacg tgaaccagac gccgtcggtg tatgcgatgg atacgtggca
     6121 tccaacgccg cggctgtcgc tgcagctggg cttccggtat gacgcgctgc cgcaggcctg
     6181 ggagcgcaac aactacatcg ccaacttcaa cccggcgcat ttttcgccca ctgcgacacc
     6241 ggcatggagg tcggatggtt cgatggaccc gaccggaccc ggattcgcaa cgtttacagt
     6301 ggacggcgca accgctcctg cctacgtgaa cggaatcgat cttgcgggcc aggcgggcgt
     6361 accgcgcgcc ctggtgagca acaactacaa cacctggcag cctcgcatcg gcttctcgga
     6421 tgacctgttc ggaaacggca agaccgtgct ccgcggcggc ttcgggacgt tcttcgagcg
     6481 gctgcagggc aacctgatct acaacagcgc gaccgtatcg ccgttctctt acggtccgtc
     6541 agcgaaccag gtgtacctga gcgacccgca caccagcatt cagtcgggcg cgacggccac
     6601 cacaccgttc ttcccgggcg gccagtacga gatggcgcag cacttcccgg atcccgcggt
     6661 tgcgatgtac agccttggac tgcaacggga gctatcgccg tcggtcgtct gggttgtgca
     6721 gtacgtgggc aacatcgcgt ggcaccagga agagtaccgg catgacaaca acttcccgct
     6781 gaccactccg atggcggtgc gggccaaggg cggaagcctc ggctcctaca ccgatgctca
     6841 caacggggca acgatcacgc tgccgacggc tggtcagggt cccacagcga acaacccgaa
     6901 ctcgttccgg acctaccagg gcctgggcga tattacggcg atggaaacca acaccaatgg
     6961 gagctacaac ggtctgcaga cagggttgcg cgtgcagaat cgctggggcc tcagtggcga
     7021 actggactac acgtactcgc acgagattga cattcagaac gcggacaacg cgtgctgcgt
     7081 gagcaatccg tggaacctga agtatgacaa gggcgcgggc aactatgacc gccgccacat
     7141 cctgcaggcg aactacgtgt acgcgttgcc gatcttcaat cactcgcagg gactggtgaa
     7201 gtccattgcc ggcgggtggg agatttcggg aaccttcatc gatgagagcg gaacgcctcc
     7261 gaccaactcc accggcatcg gcatctccgg cgccgctggc gatacagtcg gtctgggcgg
     7321 cggctatacc aatcgcgcca acgtgagcgg taaagtccac tacaccaaga aggtgacggg
     7381 ctggttcaat ccggcgcagt tctcggatcc caccccagcc tggctgggtg gtccgaacaa
     7441 cggcttcggc aacagccaca aggacgcggt tgttggtcct ggccgtgtga acttcaccac
     7501 ttcgctgtac aaatcgtttg cgatgaccga gcgggctcac atcgagctgc ggttcgagtc
     7561 gttcaacacc ttcaaccatg cggaattcca gaacctgaac acgggctaca ctcgccccaa
     7621 cagttccgga tcgtatggga acttctcgca ggtgaacaac gactggggtc cgcggttgct
     7681 ggaactcggc ggcaagatcg tgttctaacg caaacccttc aaccggggcg cggcgggtct
     7741 acctgccgcg cccttttact ttggaagcgg aagcaaaccg ggcaggagtt ttgcagggtt
     7801 tgagtcgatg gtctgcggcg ttgcgtgaga aagccgcaag caaagtggcg tcactgccag
     7861 ttacgatggt gaacgtgggg atgaaggcag tcgttttcgc ggggctttgg ggcgcgctgg
     7921 ttgcggcggg ttggggacaa cgtactccag caatggatgc gcagaagcag gcggcgctca
     7981 acctggagca tgagaacaga attcctgaag cggaggcagc ctggcgggcg tacagccacg
     8041 cgcatcctgg agaccccgag ccctgggcgc aaatgggact tctggaggca cggcaggagc
     8101 actacgcgag ggcgatccca ctttatcaga aagctctcca gctgagcccg catgtgccag
     8161 gccttcggct gaatctgggg ctggcactgt ttaaggacgg ccggtcgaaa gaagccatcg
     8221 cagagttcaa aacgttgctg aagactgcgc cgcccgcatc gcccgaagcg cagcggctac
     8281 ggctgctcat cggcatggcg tactacggga tggcggagta cacgcaggcg attccttatc
     8341 tgaaaagggc tgcggcgcag gataaggaga atctcccgtt gcgcctcgcg ctggcgcaca
     8401 gttgtctttg gtcgaaggaa tacccgtgcg tcatggaaac ctaccgcgag atcctgacgc
     8461 tgaatgccga atcggctgag gccgatatgc tggcgggcga agcgctggat gcaatgcggg
     8521 acgataccgg ggcactggcg cagtttcgtg ccgctgagaa ggcgaatccc aaagagccct
     8581 acgtacactt cgcggtggcg tatgttttga tggcgcaaaa acggtatgcc gatgcggttc
     8641 cggagtttca ggcggatctc gaagccgatc ccaggcatgc tcaggcgcgg gtctatctgg
     8701 cggactgcta tgtgcatctg gaagacaacg cgcacgcgca accggagctg gagagggcgc
     8761 tgaaggacga cccatcgatt gcgctgggcc atcttgatct cggaatcatc tacgctgccc
     8821 agggacgcaa ccaggaggcg ctggcgcaat atttgacggc gatccggttg aatccgcagg
     8881 acgccgatcc gcactggcgg ctgtcgcgac tctaccaggc gatgggggag acggagaagg
     8941 cgcgggcgga ggcggtcctg gtgagcacga tgaagaagca ggcttatcag aatctgtatc
     9001 agcagatttc cggtccggca aatcacgcag ccgaagtagc caatccgccg cagtagaggg
     9061 cttgcttgtt gctgtgatcg agctgtctct gcaggactac ggccggcttc cctttatccc
     9121 cggcagcggg acttcggagg ggccgaagcg tgaaaaaccg ggttctccgg taatcgtttt
     9181 ctggagtggc attctgtgtc tatactgaac cgggccgaag agcgtcgctg gacggcgacg
     9241 gctggccggg gaaactatga acgcaaaagt gaattccgga ctgatgttcg cggcgcttgt
     9301 gctgatcggc gcaacgacag cgatggcgca acagaatgga acaggagcca gtggggcttc
     9361 ttcgagaccg caaggtccat gcgacatcta cgccgcggcc ggggatccgt gcgtggcggc
     9421 gcacagtacg acgcgcgcac tgtatgcctc gtacaacggg ccgctctatc aggtcaagcg
     9481 gcagtcggat ggcaagacgc tggacatagg tgtggttcag cccagtgcgt cgccggttcc
     9541 ggatgcgggc ggatacgcga atgcagccgc tcaggacgcg ttctgcgcca acacgtattg
     9601 ctggattacg acgatttacg accagtctcc gaagcacaac gatctgacgc aggcgccgcg
     9661 cggtggattc agtggcccgg cgatgggggg cttcaacaac gtgcccatcg cggatatggc
     9721 gccggtgacg atcatggggc acaaggtgta tggcgtcttc attgagccgg gcatgggttt
     9781 gcgtaatgac gatccgaagg gcacggcggt cgacgaccag gcggaggggc aatactgggt
     9841 catcaatggg cgccattaca actcggggtg ttgcttcgat tacggaaacg cggaaatcga
     9901 cagccgcgac gatgacaacg gcacgatgga aaccgcttac ttcggcgatg cgctgccgtg
     9961 gtttcatggc aacggttctg gtccgtggat tatgaccgac caggagaaca acctggtggg
    10021 ctgcgtcaat gcggatggat cgaaacactg cgagaacgtg ccgagcatcg aatggcgatt
    10081 cgtgacggcg attgccaagg gcgaaccgca tcactggact tcgatgggag gcgatgcgca
    10141 gcagggcgac ctgtcggtga tgttcagcgg tgctcgcgtg aatgcgacct acgatccgat
    10201 gcggaagcag ggggcaattc tgctgggcaa cgggggcgac aacagcaacg ggtcgcaggg
    10261 cacgttctat gaaggtgcga tgacggcgtc ggggacgttt ccgacagacg ccacggacca
    10321 aaaggtgcag gccaatatcg tggccgcgca ctatggtgtg gcgccgctgc aggtgtcgcc
    10381 ggcgaaggca ggggacatgc cgcccgggtt gcagaccttc tcgccgggat cttcgcagga
    10441 gacgacggtg acgttcacga ataccactgg ggcgcctgcg acgaatgtcg aactgagcct
    10501 gactgcgccg aacgggtgga aggcggtggt ttccgggacg tcggagaaat cgaaaacctt
    10561 cgctggtcca gttacgccgg gtgggaatgt gagcgcaacg tttacggtga cagccgggaa
    10621 cgcgggcttc aacggcgatc tggtcggaaa agcccgatgg atgaatgggg cgaacagcag
    10681 ggagcaaacg gagacgatcg cggaaaaggt gagaaacgcg agtccggtga agattaacga
    10741 gttccgcatc agctccgggc ctcctgcgaa cgggacagat tcgttcatcg agctctacaa
    10801 cgcgggatcg agcagcgttg acctctcgcg ctggacgctg accgagcacc caacgcagca
    10861 ggctgttttc tcgacggtga cgattccggc agggacaaaa ctcgcggctc acggtttcta
    10921 cctattgggc ctgtcgaatt cggggctggc gaccgcggca cgtgctggcg agaagaccat
    10981 ctatgtgaga agtgtcacgg gcatgaaggc cggcgatacg gtcagcatcg gaagtggctc
    11041 gagcgcggag acacggaaga ttgctgaggc cgggacggct gcgagcaaca tgacgacgtt
    11101 gtggcagcct ctgccggatg ggctgatcac gcttccggcc ggttcgacca atgtgccggt
    11161 gacgagcgcg gccggttttg ttgttgggca gaagattgct cttggctatg gcgctacgta
    11221 tcccactgtg cccggagagg tggagaagta cgaggtcgct acggtgacgg cggttgggaa
    11281 gccgggtacg caggcgtatc tcgcgacgga tgcggctgcc ggcgcgacca acattcaggt
    11341 gacgtcggcc gaggacattt cggcgggtga caagatccgt ctggacatcg acagcgaggg
    11401 ccatgggatc gagacggtga cggtgacgcg tgtcggtacg caggcggacc actcgaatct
    11461 tgctgcgaat gcaagcgcgg gtgcgacgac gatcaaagtt cgcagcgtgc ggggctttac
    11521 tgttggcggg aagctgacga tcggcacgcc cacgaatcgg cagacggtga cgattgcgtc
    11581 ggttgggacg ggcggtccga acgggacggg cattgagttt tcaccggctc tggaacaggc
    11641 acacatcaac gacgagactg tcgtgcaacc gggcaccggc ctcgagctgg cggctccgct
    11701 gaagttcaac catgctgcga accttccctt cagcgatcgt ggaacaggga tcacgttcga
    11761 accggcgacg gcctttgctc acatgagcaa tgaaccggtt caggcgctcg gaacgggcat
    11821 cacgctcgac agtccactgg cgcacgatca cgcgatcgac gcagttgtgc gggacgcgac
    11881 ggtcaagacg gctggatatc agggcaatcc ggcgcccaac cagtggttcg gcggcccgga
    11941 acttgccgcg aacgttccgt ttttcaactt cacgatcacg cgcagagaag ggagcatgat
    12001 gctgcgggat gcggcgggcg tggttgtgga cagcctgaat tacggagggc tggtggatcc
    12061 gtggctgggc gaggggtatc aggccgaatc cggagcgcag cataatggtt gctacgtgac
    12121 ggcgcccgga tgggcgggcg cggtgtggcc gccgatgccg acggccggcg ggtcgaatac
    12181 gagtgcgggc cgcttgccgg acggagccga cagcgacagc aactgcaatg acttcgtgac
    12241 gtcgccggct acgacgctgg gggtcgcctc cacaacgggc gcgaccaata ttaaggttgc
    12301 gggtgtggaa gggttcgcgg cggggcagac gatccggatc gtggaaggtg cggacgcgga
    12361 aacggtcgag atcgcgacgg tgggcacgcc tggcgcaacc acggcgactg cggcgaccga
    12421 agcaggcgcg actgtcatcc acgtcgggaa cccgattgga tttgaaaacg acgagaccat
    12481 cacgatcggg agcggagacg atgcagaaac ggcggaggtg gtgcggcgaa cccgcgaatc
    12541 caccatcgtg gtgaccgcgc cgctggcccg ggctcatgcg gcggggacag cggtgtcggg
    12601 aagcggcatc acgctgcgtg ctcccctgag gcgcgaacat gcggctgagg cgcaggtgac
    12661 cggcagcatt tccacgccgg gggcgccaaa ccaatatttt cggaagtgag ggacaggggt
    12721 ccctttaaat cggtttctga cccgccaatg gccggacgac cttaggtgcc cttaaaaata
    12781 ccgccacaat atttggtcgc catgacggca tgcgaatcga gcgctggcgg cgaccgtggc
    12841 gcttcaacga ggccagcggg attcgatcgg gggctggtcc aaacccggcg acacccgttc
    12901 gcgtccagat tggcgacggt atagcagcgg gcggcactgg cccggagcag cgattccatc
    12961 gcgtcaacca cgcacaacca tcacaaagct cggggtgcgc ttgacacctc tgtgaattcg
    13021 cgcgtaggat gcgttacgtt aacgttaaag tagtcgttga tcgaaaacag gcagaccccg
    13081 ttccccagtc tcaaatcagg tcctgccaca gtgccgtgaa gcaaattttc gcaaggaggc
    13141 agttatggga gagtccatca gacagtgctg gaatcggctc tggttgctca cagtgccgct
    13201 gattctcctg ggcgttgccg ggttctcgtc cccgctcgcc atagggcaac agaccaacgc
    13261 caatgtcaac ggagtcgtta aggatccgag cggcgccgca atccccaatg cgcgcgttga
    13321 gcttacgaac gtgaatacgg gtgtcgtgag gacaaccact acgaacaacg acggagccta
    13381 taactttccg agcgtggtgc ccggggttta cacgatgcag gcatccgcca ccggttttgc
    13441 ggcagtgtcg caaccggcgg tcacgcttca ggtcagccag attgcgacct tcgattttga
    13501 attgaaagtc ggttcgacca ccagcagcat taccgtgact gccacggcgg ccgcgttgca
    13561 gacttcgagc tcggaactgg gcacggtagt taccaccaac caggtcaacg acctgcccct
    13621 gaacgggcgc aattttaccc agttgctgac gatcacaacg ggcgtcgcga acgtcaatac
    13681 ggaccagaac ggcaatggcg gcggcggctg gaatggtcac acggtgggga ccaccgacat
    13741 cccggccgtt aacggcgcgg ccaaccggag caatgtcttc ctgctggatg gcgcaaacga
    13801 cctgaacacg ctgtccgggg tctataacta ttcgccgatc gtggatgcca tccaggaatt
    13861 caagacgcag gggcataacg atcttgccga gtatgggggt gccgccggcg gtctcgtaac
    13921 ggtggtcacc aaatcgggaa cgaatcagta tcatggcgct ctttgggagt tcctgcgcaa
    13981 cgagcagatg gattcccgcg gctactttga gtccagccgc gcgccgctgc ggcagaacca
    14041 gtatggcgct tctgccggcg gtcccctttc cattcccaag ctgtacaacg gtaagaatcg
    14101 cactttcttc tacgctgcgt gggagagcta ccgctacgcc tctgcccagg aaacgggagc
    14161 gctcggacca acggctgcga tgcgcgccgg cgatttcagt gccctggggg tgcccatcta
    14221 tgatccggcc acgaccactc ttgaccccgt ttccggccag tacacgcgcc agaccttcac
    14281 gcaggagtac aacgaaggtc cgggcaatcc ctccctgtgc ggaggcgaca ctaactgcat
    14341 tcccagcagc cggttcaatc cgatcacatc gctgtacgcg gggctcattc cgaacagcgg
    14401 tgctctggtc aacgggagta acgtcttcgt gaccggaaga tcgttcacga atcagcattc
    14461 gggtacgctg cgggtggatc agaacttcgg caacaacgac cagctgatgt ttcgttacag
    14521 ccagttcgat cagtacgggc agtctccgtc gggcacgatc ggttcgggct tcgacagtgt
    14581 tgtcgggcat aactacatcg gtcattggac gcacacctac agtccaacga ccttttccga
    14641 tgtttacttc ggaagaaact acggatatag cctgatcggc acctcctggg ccggtgagga
    14701 tgcagcgttt cttggccagc tgcagtcact ggggatgtcc tcagcctgga tgaccctcga
    14761 caacaagacg tacgctccgc agctcgcagc ggacagttat gtcggtttga ccggttcgca
    14821 gttgcaggga agcggcctgg cggatgactg gcagttcggc ggttcattct ccaaaattct
    14881 cggcaagcac acgctcaaag ccggtgtcga tatcgaaacc aacaacttca cttcgccaat
    14941 tgcatattcg ggagtttatt ttggggtgaa gcagacggcg ggcgtcggag ctgagcaggg
    15001 agtaggcgga aacagctggg catcgttgtt gctgggcgtt ccccgcgaag ccaactatcg
    15061 gaatatcgat gaggtggcca agggcggctg gatggatgcc ttctacgtgc aggatcagtt
    15121 caaggcgacg agtcgtctta ccctcaatct cggcttccgc aacgacatgg tgttcacgcc
    15181 aatttatggg acgggcaagg gcgggaacta ctacaccggc aacgccgacc ccgttacggg
    15241 ccaatacatc ctgaatgcgc gcccacccga ctgctcggca acccagggtg cgccgtgtat
    15301 tcccaacggc atctataccg catcgagcac gccggctccg ggcgggcttc ccgctcacac
    15361 catcgttaac ccgagtcttc gcgtgattca aaactcactg gcggactggg gtctccggct
    15421 gggtgcggcc taccgtttga atgacaagac gacgattcgc ggtggatacg gccgtgagta
    15481 cgacgagtgg gccaccatcg tccagctttc gcagaatttc ggcggaaact ggcccgcggt
    15541 caacactctc gacaacgccg gtctgaatac caacgttccc acggcgactg cggccgatcc
    15601 gctgcaattg ggtggcggcg gcgcaatcgt ttaccccatc aatgatttca gccaggtcag
    15661 ccagtggatg gtggatccga acttcaaaac tccgtattac gaccaataca acgcgggcat
    15721 tgagcggcag ttaccgggga acctagctct cgacgcgaac tatgtcggag ccaccggcag
    15781 ccgcgaggac tggggaccaa ccatgaatac accccagccg ggaccgggcg atgttcaaag
    15841 ccgtcggccc tacccctata tgcagcagca gtggtttgac cagagcgttg gacatagtca
    15901 gtacaacgcc ctgcaggtca ccctcaacga acggaacctc catggtgtcg gctttctggt
    15961 cgcgtacact ctggggcact cgaattccga tggctgcgga ctgggtgcaa gctgcgactc
    16021 atccaaccca tatcaccgca gcgtcgatta cggcacttcg gatctgaacc aaaagaacat
    16081 cttctcggcc gcatttacgg cgcagtcgcc cttcactcac tctcccgata aggtggtatc
    16141 cggtctggca ggtggatggt cgatgaacgg cattgtgcag ttcagttccg gacaggttta
    16201 caccgtaacc accggcagcg acccagagaa tatcggctgc tgcctgcagg agagggtgaa
    16261 cgtgacgggt gacccgaaca gtgggtcggg tctccatact ccggcgaaat ggttcaacac
    16321 gagcgcgttt acgcagccgg cggcctatac ctacggcacg gaaaaggtaa acccgtttac
    16381 tgcgcaaatg cgacacgaag tcgacctgtc cctcttccga cagttccaca tagggctcgg
    16441 agaagagaga tacttcgaat ttcgcgccga ttcgttcaac ctgttcaaca acgttgtctt
    16501 cgatcgtccg gatgccacga tttccgacac gactttcgga caagtcacac ggcaacagaa
    16561 ccagccacga caattgcagg tcgctctgaa gttctactat tagcccgtgg cctaaccagc
    16621 caaccggcat ccctgccgcc aggacggcag ggatgccagg ttgccttcga agctccgatc
    16681 gatgcaagct aaagattcaa gagtgacgga tgcgttcggc gctgacccta ctgttttccg
    16741 ccgcagtgct ctgcggattt ttgccggctc agagcatcgc ggcgccgcca atccggttcg
    16801 aggacatcgc tgagcgcgcg ggtgtgcatt ttgtcgtcga gaattctcct accccggaaa
    16861 aacatcagcc ggagaccatg cctgccgggg tggctttgtt cgattatgac ggggacggcc
    16921 tgctcgatat ttatctggtc aacggcgccg agatgccatc gctggtgaaa accgggccga
    16981 agtactacaa ccggctgttc cacaacaacg gcaatggaac cttcaccgac gttacggagc
    17041 gcgccggagt cgcgggagcg ggttatggaa tgggcgccgc cgccggcgat tacgataacg
    17101 acggccggga cgatctgttt gtggccaacg tcaacggaaa tcaactgttc cacaataatg
    17161 gcgacggtac ctttaccgac gtcaccgtga aggccggtct gagtggcgcc gtgcacaacg
    17221 gccgcaagat gtggtccatc gcagcaggct g
//
LOCUS       DASZYN010000261         2438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_717954, whole genome shotgun sequence.
ACCESSION   DASZYN010000261 DASZYN010000000
VERSION     DASZYN010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717954"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1752
                     /locus_tag="VHE33_06810"
     CDS             160..1752
                     /locus_tag="VHE33_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97201.1"
                     /translation="MAGAQEKGNEKGAEAKPGAASTVGAPDSTTEGSVTIGGQAVAYR
                     AVAGTITVGANDQQDATLAFDGSLLPNSGVKPPTDPAEAPPTARIFYVAYFRKDATDE
                     THRPVMFIYNGGPGSATMWLHMGTFGPKRIVLPDEDHQEGAPYSIVSNQYSLLDASDL
                     VFIDAPGTGFSRTFGKDRGKAFYGIDQDGQAFSHFIRKFLTKYGRWNSPKYIFGESYG
                     TPRSAVLSADLNGVDLNGVILLSQILSFDNSIDGPEANPGVDQAYALGLPTMAATAWH
                     FHKLANQPAALGPFLQEVEKYALTDYMEALLQGSELSDEQKHAVAEKLHGYTGLPVDY
                     LVRANLRVNGGEFSKELKLDEMTTVGRLDTRYQGPDMNPLSAETDYDPQSDAITSGWL
                     AAINTYLRQDLKYGAQETYFPSGRMGGDFNWDMHHRTPGFRGFSSDWSGPNVMPDLAY
                     RMKMNPKMKVMLAGGYFDLATPYFEGEYEMHHLPMPNSLQKNISYHYYEAGHMIYVNE
                     DVLKQFHSDVEGFVRATENGAK"
     gene            complement(1787..2152)
                     /locus_tag="VHE33_06815"
     CDS             complement(1787..2152)
                     /locus_tag="VHE33_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97202.1"
                     /translation="MDAPVLMQKLMEIERALSRHDECAVRNLIIAVQDDVLALEQENE
                     ALALENAGLRQRLSAARHATMPFAAHSRAFPGSSGPEPPRERLCADEEPAPNSEAAAP
                     DPNDDRPPRTWRITHFFFS"
BASE COUNT          477 a          632 c          891 g          438 t
ORIGIN      
        1 cgaaaatcac cagagcacgc cgaatccggt tcttacccgt gcagtgcggt tatactcggg
       61 ggcattccaa aaaaggacga ggtgagcatg gatttgcgtt cgtggagccg ggccgcggta
      121 cttcctttat tcgtagcagt cgcatgcacg ttttcctgtg tggcaggggc acaggaaaag
      181 gggaacgaga agggcgcaga ggcgaagccg ggggccgcct cgacggtggg tgcgccggac
      241 tctacgaccg aagggtcggt gacgattggc gggcaggcgg tggcgtaccg cgcggtggcg
      301 gggacgatca cggtgggcgc gaacgatcaa caggatgcga cgctggcatt tgatggcagc
      361 ttgctgccca attccggcgt gaagccgccg accgatccgg cggaggcgcc gccgacggcg
      421 cgaatctttt atgtcgcgta cttccggaag gatgcgaccg acgagacgca tcgccccgtg
      481 atgttcatct acaacggcgg ccccggctcc gcgacgatgt ggctgcacat gggaacgttt
      541 gggccgaagc ggattgtgct gccggatgag gatcaccagg agggcgcacc gtattccatc
      601 gtgagcaatc agtattcgct gctcgacgcg agcgatctgg tcttcatcga cgcgccgggc
      661 acggggttca gccggacgtt tgggaaggat cgcggcaagg cgttctacgg catcgatcag
      721 gacgggcagg cgttcagcca cttcattcgc aagttcctga cgaagtacgg gcggtggaac
      781 tcgccgaagt acatctttgg agagagctac ggcaccccgc gttcggcggt gctgtcggcg
      841 gatctgaacg gcgtggatct gaacggggtg attctgctgt cgcagatcct gagcttcgat
      901 aactccattg acggtccgga ggcgaatccg ggcgtggatc aggcgtatgc gctggggctg
      961 ccgacaatgg cggcgacggc gtggcacttc cacaagctgg cgaaccagcc ggcggcgctg
     1021 gggccgttcc tgcaggaggt ggaaaaatac gcgctgaccg actatatgga agcgctgctg
     1081 cagggcagcg aactgagcga tgagcagaag cacgccgtcg ccgagaagct gcacggatat
     1141 acgggactgc cggtggatta tctggtgcgc gcgaacctgc gcgtaaacgg cggcgaattt
     1201 tcaaaggagc tgaagctcga cgagatgacc acggtgggcc gtctggacac gcggtatcag
     1261 gggccggaca tgaatccgct gagcgccgaa acggattacg atccgcagag cgacgcgatt
     1321 acgtcgggat ggttggcggc gatcaacacg tacctgcgcc aggatctgaa gtacggagcg
     1381 caggaaacgt attttcccag cgggcgcatg ggtggcgact ttaactggga catgcatcat
     1441 cggacgcctg ggttccgcgg cttctcgagc gactggagcg ggccgaacgt aatgcccgat
     1501 ctggcatacc ggatgaagat gaatccgaag atgaaggtca tgctggccgg gggatatttc
     1561 gacctggcga cgccgtactt cgagggcgaa tacgagatgc atcatctgcc catgccgaac
     1621 tcgctgcaga agaacatcag ctaccactac tacgaagccg gacacatgat ctacgtgaac
     1681 gaggatgtgc tgaagcagtt ccacagcgat gtggaggggt tcgtgcgggc tacagagaac
     1741 ggtgcgaagt aaagcagtcg gcaggcaaag gcaatcgcgc gttcggtcag ctgaagaaga
     1801 agtgagtgat gcgccaggtg cggggcggcc ggtcgtcgtt gggatcgggc gccgccgcct
     1861 cagagtttgg cgcgggctct tcatcggcgc agaggcgttc gcggggaggc tcagggccgc
     1921 tggagccggg gaacgcgcgg gagtgggcgg cgaagggcat cgtggcgtgc cgcgcggcgc
     1981 tgaggcgctg gcggaggccg gcgttctcga gagcgagggc ttcgttttcc tgctccagag
     2041 cgaggacgtc atcctggacg gcaatgatga ggttgcggac ggcgcattca tcgtgacggc
     2101 tgagggcgcg ctcgatttcc atcagcttct gcatgagaac gggggcatcc atgaggcacg
     2161 ctccggggga ggaactgggg atagagtgcc ttgggtgggt tggggaggct gtgatcgatg
     2221 tcacgggggt acagagtaca gggtggaggg attgttggcc cggtagttca atctcagcgg
     2281 tttagacaga ttccttgaac tttcggcgtc ccgaaactga attcgggacg ccgagttgtt
     2341 tccggcttag ttgttgttgt cgggtgggag gaagagccac ggcgtcgtcg tcacgcctgt
     2401 gccggtgacc gaggcgcagc ggccggagcc ggggccgg
//
LOCUS       DASZYN010000262         2319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_268229, whole genome shotgun sequence.
ACCESSION   DASZYN010000262 DASZYN010000000
VERSION     DASZYN010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268229"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VHE33_06820"
     CDS             complement(<1..232)
                     /locus_tag="VHE33_06820"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVT97203.1"
                     /translation="MSAATQTSTPHTASTFPFTLTEDQESLQREIRDFAAREIAPNVL
                     RWDEASEFPMEVVKQLGRMGLLGVIFPTEYGGA"
     gene            348..605
                     /locus_tag="VHE33_06825"
     CDS             348..605
                     /locus_tag="VHE33_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HVT97204.1"
                     /translation="MPTRNVNLTPELDRFVHSRIKTGRYENASEVVRAALRSLEREEA
                     EHEAKLAALREAIDEGESSGFVTGDVFARVLKNARPLRTRK"
     gene            608..898
                     /locus_tag="VHE33_06830"
     CDS             608..898
                     /locus_tag="VHE33_06830"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HVT97205.1"
                     /translation="MTWIRFTARAEADLTRIADYTLHMWGETQKVRYVRSLQACCVRL
                     EENPMLGRPCDEIRPGLRRMEEGKHVIFYRPEKNGIAVYRILHQSMVPKDRV"
     gene            complement(914..1870)
                     /locus_tag="VHE33_06835"
     CDS             complement(914..1870)
                     /locus_tag="VHE33_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97206.1"
                     /translation="MTLRRLVLVLAVLLLPCAIVAQTAPIEIPLEYSGRHLYVTVTDE
                     PLGPLTLMLDTGFQRTTLAASVARKGEVHTHFLERSLSYNGFGGGKAKRRYLTTDVSL
                     RSGTNVIFGGSALVTDLGDLNKGFGHAFDGFLGWDFFRQWCVTLEYAPARLTLRGQCS
                     PPLGAYANLHGDWNPQGLLLTSQITFVNGHKADALLHMDTGSDITLLLNTRFREAAGL
                     KGSASSAQESHGLGVNGRYTTDLLPIAKIDLNGQLQLAPGKDTMIAIGRAGAFTKVHW
                     WEGPSAVKINRDGVIGNALLDRFRWTFDPGRKKIYAVPAGEK"
     gene            complement(1911..>2319)
                     /locus_tag="VHE33_06840"
     CDS             complement(1911..>2319)
                     /locus_tag="VHE33_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVT97207.1"
                     /translation="RHVALSNVALEYVERARKITPIVSVQNRYSFADREWDFVLDFCE
                     KNGIAFIPWAPLGQVRAAAHELLEKFARQLSATPLQVALAWLLRRSPVTLPIPGTSSA
                     KHVEENVAAAALKLPDAMFKQLDAVNPPPASLR"
BASE COUNT          485 a          730 c          664 g          440 t
ORIGIN      
        1 ctgcgccgcc gtattccgtc ggaaaaatca cccccagcag tcccatccgc ccgagctgct
       61 tcacgacctc catcgggaac tccgaggctt catcccagcg cagcacgttc ggtgcaatct
      121 cgcgtgcagc gaaatcgcga atctcccgct gcagagattc ctggtcctcg gtgagggtga
      181 acggaaaggt ggaggcggta tgcggggtgc tggtttgggt cgcggcactc atgcgtcgcg
      241 gtactccggt gggagagatg gagaaaacgg gtaagagtag cagagtcgag tgggttttgt
      301 cgctgacttc tattgacaaa gattgccaat cgcgaaaaca ttgatgcatg cctacacgga
      361 acgtaaacct gacgcctgaa ttggatcgat ttgtgcattc gcggatcaag accggccgtt
      421 atgagaatgc cagcgaggtg gtacgtgcag cactgcgcag tctcgagcgc gaggaggcgg
      481 agcacgaagc gaaactcgcg gcgttgcgag aggccatcga cgaaggggaa agtagcggtt
      541 ttgttaccgg agatgttttt gcgcgtgtat taaaaaacgc acggcctctg cgcacgcgca
      601 aatagcgatg acatggatcc gatttactgc tcgggcggag gcggatttga cccgaattgc
      661 agactacact ctgcacatgt ggggcgaaac ccagaaggtt cgctatgtcc gatcccttca
      721 ggcatgttgc gttcggctgg aagagaatcc gatgctgggg cggccttgcg atgaaattcg
      781 tcctggtctc cgccggatgg aagaggggaa gcatgtaatc ttctatcgtc ccgagaagaa
      841 tggcatcgcg gtctatcgca ttcttcacca aagcatggtg cccaaagatc gcgtttgagc
      901 cattgagatc tcgtcatttc tcccccgcag gcaccgcata aatcttcttc cgccccggat
      961 cgaacgtcca ccgaaatcgg tccagcaggg cattgccaat cacgccgtcg cggttgatct
     1021 tcactgccga agggccttcc caccagtgca ccttcgtgaa tgctcccgcg cggccgatcg
     1081 cgatcatcgt gtctttgccc ggcgcgagct gcagctgccc gttgagatcg attttggcga
     1141 tcggcagcag atccgttgtg tagcggccgt tcacccccag gccgtgggac tcctgcgccg
     1201 aggaagcgct ccccttcaaa ccagctgcct cgcgaaaacg agtattcagc agaagcgtga
     1261 tgtcactgcc cgtatccatg tggagcagcg catcggcttt gtggccgttc acgaacgtta
     1321 tttgcgaagt cagcagcagt ccctgggggt tccaatcgcc atgcaggttg gcataagcgc
     1381 ccaacggagg cgaacattgc ccccgaagcg tcagccgtgc cggcgcgtac tccagcgtca
     1441 cgcaccactg gcgaaagaag tcccacccca gaaacccgtc aaaagcgtgg ccaaacccct
     1501 tgttcagatc gccgagatcg gtgaccagcg ccgatccacc aaaaatcaca ttcgtccccg
     1561 agcgtagcga gacgtccgtc gtcagatagc gccgcttcgc cttgccgccg ccgaagccgt
     1621 tgtacgacag cgatcgctcc aaaaaatgcg tgtggacctc gcccttcctc gccaccgacg
     1681 cggccagcgt cgtccgctgg aagcccgtgt ccagcatcag tgtgagcggt cccagaggct
     1741 catccgtaac ggttacatat agatgccgcc ccgagtactc caacggtatc tcgatcggtg
     1801 cggtttgcgc gacgatcgcg cagggcaaga gcagcacagc aaggaccaaa accaggcgcc
     1861 gcagggtcac cccattacgg ctgtcccggg aatcgaattc caactgcacc ctaccgcaaa
     1921 ctcgcaggcg gcggattcac cgcgtccagc tgtttgaaca tcgcatccgg cagtttcagc
     1981 gctgctgctg caacattctc ctccacatgc ttcgccgacg acgtccccgg aatcggcagc
     2041 gtgaccggcg agcgccgcag cagccaggcc agcgccacct gcagcggcgt cgcgctcagc
     2101 tgcctggcga acttctccaa cagctcatgc gcggccgcgc gcacctgccc cagcggtgcc
     2161 cacggaatga acgcaattcc attcttctcg cagaagtcga gcacgaagtc ccactcccga
     2221 tccgcaaagc tgtaccggtt ctgcaccgac acgatgggcg taatcttccg cgcccgctcc
     2281 acgtattcca gcgccacgtt cgacagcgcc acgtgccga
//
LOCUS       DASZYN010000263         2973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_138941, whole genome shotgun sequence.
ACCESSION   DASZYN010000263 DASZYN010000000
VERSION     DASZYN010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138941"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VHE33_06845"
     CDS             complement(<1..506)
                     /locus_tag="VHE33_06845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HVT97208.1"
                     /translation="MNLLPIFLRLDGKPVLVVGAGTVALAKIESLLASRAKVTVVGPK
                     TVPRIRELAHQDAVVWHQRLFEPQDLEGAFLVIAATNDSAVNRTVYEEARRRNIPCNA
                     VDDPPNCDFYFGSIVSRGDLQVAVSTAGESPALAQRLRQEIDAQLPQDLGPWLKELGG
                     LRGEVRAAT"
     gene            complement(503..2194)
                     /locus_tag="VHE33_06850"
     CDS             complement(503..2194)
                     /locus_tag="VHE33_06850"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HVT97209.1"
                     /translation="MAELSAVKETKAQKAERLKREKNPWDAIEEIRAFARNGHASIPA
                     DWSTYFRWWGIYSQGDGLGVTGGKGGEGKATEYFMMRVGVPNGILRSDQARAIADIAR
                     THGRNLADITVRQNIQFHWLTVESLPEVMEALGRVGLSPKAACGDVVRNVTGCPLAGI
                     AHDEIVDASPLAVEISRALQGNNDFFNLPRKFKISITGCGVWCSYPEINDIGLTAIRR
                     GKEIGYSVRVGGGLSNEPHLAVRLNAFVKTDQAAAVVRAIAEIFRDQQGLRESRDRAR
                     LKYLFMKEGWTAEQFLAEIEHRIGYKLDPAVDEVIPDDILRDHAGIHPQKQFGLSYVG
                     ASVLRGRVTGDQLAAAAALSERFGTGDLRLTVMQNLVFLNIPSHRAAELAGELGKIGL
                     NVQGSQFWRGGIACTGTEFCKLAITETKGFLRWLVEEMEVRLPEFDQQLRLHVTGCPN
                     SCGQHWIADIGLEGKKVKHEGQLTDAYYFCVGGSVGKHAGVARPVGFRTPAHRVPEAL
                     ERLLRKYLEEREAGENLRAWFGRRADGELRAALAGEEFIPVERDVPSGRVPVEVG"
     gene            complement(2428..>2973)
                     /locus_tag="VHE33_06855"
     CDS             complement(2428..>2973)
                     /locus_tag="VHE33_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97210.1"
                     /translation="TMAFDQRFAMVLVGSSGKGGATPLRRNYGEGVENLATGEYYWMD
                     GNFMKYDASEATFGSMNPGQLPVDSNELIALCAPRLTFISYGVPEKGDAHWLDHQGSW
                     MATVDANRVWELLGAKGLSGGKDFTLDPNYHTTKMPPVNDGLLDGKLAWRQHDGGHTD
                     APNMTFFITWADKFIDHKAGQ"
BASE COUNT          567 a         1026 c          819 g          561 t
ORIGIN      
        1 gttgccgcgc gcacttcgcc ccgcagtccg cccagctctt tcagccacgg acccaaatcc
       61 tggggcagct gggcatcgat ctcctggcga agccgctgcg ccagcgccgg gctctccccc
      121 gcggtcgaca cagcaacctg caaatcgccg cgcgagacga tcgagccgaa atagaaatcg
      181 cagttcggcg gatcgtcgac ggcattgcag ggaatgttgc ggcgtcgcgc ttcttcataa
      241 acggtacgat tcactgcgga atcgttcgtg gccgcaatca ccaggaaagc gccctcgaga
      301 tcctgcggct caaacagccg ttgatgccac accactgcat cctggtgcgc cagctcacgg
      361 atacgtggaa cggttttcgg tccgacgacg gtcactttcg ctcgcgaggc cagcaggctc
      421 tcgatcttcg ccagcgctac cgttcccgca cccaccacca gcacaggttt gccatcgagt
      481 cttaaaaaaa tgggaagcag gttcatccca cctccaccgg aacgcgcccg ctcggcacat
      541 cgcgttccac cggaataaat tcctcgcccg ccagcgccgc ccgcagctcc ccatcggcac
      601 gccggccaaa ccacgcgcgc aggttttcgc ccgcctcgcg ttcctccaga tacttccgca
      661 acagccgctc cagcgcctcc ggcaccctat gcgctggcgt gcggaatccc accggacgcg
      721 cgacgcccgc atgtttgcct accgacccac ccacgcagaa gtagtaagca tcggtgagct
      781 ggccctcgtg cttcaccttc ttgccctcga ggccgatgtc ggcgatccag tgctggccgc
      841 agctgttggg gcagccggtc acatgcaggc gcaactgctg atcgaactcc ggcagccgca
      901 cctccatctc ttcgaccagc cagcgcagga accccttcgt ctccgtaatc gcgagcttgc
      961 agaactcggt gcccgtgcag gcgatgccgc cgcgccagaa ctgcgagccc tgcacgttca
     1021 ggccgatctt cccaagttcg cccgcaagtt ccgcggcgcg atggctgggg atattcagga
     1081 aaaccaggtt ctgcatcacc gtgagccgca gatcaccggt tccgaatcgt tccgacaatg
     1141 cggccgccgc agccagctgg tcgccggtga cccgtccgcg cagaacgctc gcacccacgt
     1201 agctcagacc gaattgtttc tgcggatgga tgccggcgtg atcgcgcagg atatcgtcag
     1261 gaatgacttc gtcgaccgcc ggatccagtt tgtaaccgat gcggtgctca atttcggcca
     1321 ggaactgctc cgccgtccat ccctccttca tgaagagata cttcaggcga gctcggtcgc
     1381 ggctctcgcg caatccctgc tgatcgcgaa aaatctccgc aatcgcccgg accacggcag
     1441 cggcctgatc cgtcttcaca aaagcattga gacgcaccgc aagatgcggt tcattcgaca
     1501 acccgccacc cacacgcacc gagtagccga tctccttacc gcgccgaatc gcggtcaggc
     1561 caatgtcgtt gatctcggga tacgagcacc acaccccgca acccgtgatc gaaatcttga
     1621 atttgcgcgg caggttgaag aagtcattgt tgccctgcag ggcgcgagaa atttcaaccg
     1681 ccagcgggga agcatcgacg atctcatcgt gcgcaatgcc tgccagcggg cagccggtca
     1741 cgttgcgcac cacatcgccg cacgctgctt tgggggaaag cccaacgcgg ccgagcgcct
     1801 ccatcacttc cggcagcgac tcgaccgtca gccagtgaaa ctgaatgttc tggcgaaccg
     1861 taatatcggc cagattccgt ccgtgcgtcc gcgcaatgtc tgcaatcgcc cgcgcctgat
     1921 cggatcgcag gatgccgttg ggcaccccga cacgcatcat gaagtattcg gtcgccttgc
     1981 cttcgccgcc cttgccgccc gtgacgccaa gaccgtcgcc ctggctgtag ataccccacc
     2041 agcgaaaata agtgctccag tcggccggga tcgaagcgtg cccgttgcgt gcgaatgcgc
     2101 gaatctcctc gatcgcatcc cagggattct tctcccgctt cagccgctcg gccttctgtg
     2161 cttttgtttc cttcactgcg gagagttccg ccatcgatga ctccgtaagt tttgagggaa
     2221 aataaaaaac ccgcgtcagc agatcgctga tgcgcgggaa tgatggtgtg aacttaaggc
     2281 tagaggatca cacacccatc cggcgcagcg cgcaacaaca agcgcaacac atcaccgggt
     2341 tggggcaaaa tgccattcgt ctgatagtag aggagtaacg ctgggcggtc aagccgaaag
     2401 gggctcctcc ccgctccgat gccacattca ctggccggcc ttgtggtcga tgaatttgtc
     2461 cgcccaggtg atgaagaacg tcatattcgg cgcgtcggtg tgcccgccgt catgctgtcg
     2521 ccacgccagc ttgccatcca gcaggccgtc attcaccggc ggcatctttg tggtgtggta
     2581 attcgggtcg agggtgaagt ccttgccgcc actcagtccc ttcgcgccca gcagttccca
     2641 aacccggttc gcgtccaccg ttgccatcca gcttccctgg tgatccagcc agtgcgcgtc
     2701 gcccttctcc ggcacaccat agctgatgaa cgtgagccgc ggcgcgcaaa gcgcgatcag
     2761 ctcgttgctg tcgaccggca gttggcccgg attcatgctg ccgaaggtgg cctccgacgc
     2821 gtcgtacttc atgaagttgc catccatcca gtagtattca ccggtcgcca ggttctccac
     2881 gccttcgccg tagttgcggc gcagcggtgt cgctccgcct ttgcccgacg agcccaccag
     2941 caccatcgca aagcgctgat cgaaggccat cgt
//
LOCUS       DASZYN010000264        10791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_11736, whole genome shotgun sequence.
ACCESSION   DASZYN010000264 DASZYN010000000
VERSION     DASZYN010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 10791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10791
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11736"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VHE33_06860"
     CDS             <1..910
                     /locus_tag="VHE33_06860"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVT97211.1"
                     /translation="YQKGKLQFDDPVAKYLPAFGANGKQDITIRELLTHYSGLPPDLP
                     LAEPWEGHGTAYRMAFAIAPAGPPGEKFVYSDINFIVLGALVEKLSGMTLNEYAERFI
                     FQPLGMRHTRFLPPESWIPHIAPTQYEHGASAYGVAGLATGPGDVMLRGVVHDPTSRR
                     MGGVAGHAGVFSTADDVAVYAQNLLNRLAGRPSRFPLSRRVLQLMVKPEQPAGGKAVR
                     GFGWDIDSPYSSNRGSIFPVGSFGHTGFTGTSLWMDPASDTYVIVLANSVHPNGPKRI
                     VALRGKIADAAARAVGAEKHAAASRR"
     gene            complement(974..1576)
                     /gene="lexA"
                     /locus_tag="VHE33_06865"
     CDS             complement(974..1576)
                     /gene="lexA"
                     /locus_tag="VHE33_06865"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HVT97212.1"
                     /translation="MAITRRQKEVLEFISGFVQRNGYSPSFEEIAHGLDLRSLATVHK
                     HVTNLQNKGLLARAHNRSRSLDVLPPRSKGRGVDRLPLAGRIAAGYPVEAAEIPESIS
                     LADIVGNRDVFALQVRGESMRDEHILDGDFVLVERTATARSGEIVVALVRGSETTLKR
                     YYREGSVIRLQPANIEMDPILVPAAQVAIQGRVLGVLRKY"
     gene            1762..2487
                     /locus_tag="VHE33_06870"
     CDS             1762..2487
                     /locus_tag="VHE33_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT97213.1"
                     /translation="MRLLIAEDDPALGRFLARGMEQDGHEVTMATNGEMAMEAMRNDL
                     PEMAVLDLNLPRRDGTEVLEFLRTLTEDVPVLILTARKEPEVRLRCLEMGADDFMQKP
                     FSFAELRARCRALQRRRSPGLVLRQGDVEMKRVERLVTCQGKAVTLTTREFALLEYLL
                     LHRGRAVSRATLLREVWNMSEAAGTNVVDVYINYLRRKLESAGAGAAANGSCDGETLI
                     RTVRGVGYAIGRETGTAPAATAV"
     gene            2497..3792
                     /locus_tag="VHE33_06875"
     CDS             2497..3792
                     /locus_tag="VHE33_06875"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HVT97214.1"
                     /translation="MGSIAQSRPGSGTRTAVLVSADAAFRERLRRALSELRWQVRETA
                     GGAETLACLEASPAETVVVDSWLPDLEIQEFVTEFRKVHPEVDLVMADASDEDRSEVR
                     GPRRNEIFLALRRAQATDDAIWNTAPRTIDAGGCEERVTSGESRVLQEAGQAPPTVRA
                     AGELPSGAAMHASGSVPWAAGLPEFVGSHPAMLEVSRRIRLVAPRATPVLIEGPTGSG
                     KEMVARAVHRLSPRAMRRMVALNCAAIPEGLLEAELFGHTRGAFTGAVDNRTGRIEAA
                     HGGTLFLDEIGEMPLGLQAKLLRFLETGELQRVGNNESIHVDVRIVAATHRPLGKLVK
                     AGTFRADLYYRLAVFLIRTPALAGRDQDVTALIGHFLTKMGKSSPAKQMTEAALVRLA
                     QHTWPGNVRELEHVLERAWILAEDRPRIDRDEIEFDEEG"
     gene            3865..4239
                     /locus_tag="VHE33_06880"
     CDS             3865..4239
                     /locus_tag="VHE33_06880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97215.1"
                     /translation="MQILTPMAEALNRYLDLSSQELKLTAQNMANVDTPGYRTVGFDF
                     AGEMSRSLDGLRGTAGAPAADAPVHPVRIDGLLERPDGNNVSMDREGMKMAEAQLQFQ
                     TGVELLKHAFSRVENAIRMDEK"
     gene            4369..4815
                     /gene="flgC"
                     /locus_tag="VHE33_06885"
     CDS             4369..4815
                     /gene="flgC"
                     /locus_tag="VHE33_06885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HVT97216.1"
                     /translation="MNLFGVMEVSGSALQAERIRAEVVAANMANAETTRTADGGPYRR
                     QHVVFTSEAAQGSFGAHLLANPGQADGEIGGVRVSAVVADEGAPLRRFDPGHPDADQD
                     GYVSYPDINPLTEMVDLMGATRAYGLNSSAVQAEKGMLTASLEILK"
     gene            4827..5129
                     /gene="fliE"
                     /locus_tag="VHE33_06890"
     CDS             4827..5129
                     /gene="fliE"
                     /locus_tag="VHE33_06890"
                     /inference="COORDINATES: protein motif:HMM:NF014143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HVT97217.1"
                     /translation="MQTAGIGHSLAAVLTTPAAPMGSVATEAAPFGDLLKSAIAGVDG
                     LEQQASAAVEGLMRGSGVDLHDALIATQKADMSFELALAVRNKAVAAYQQVMGMQF"
     gene            5150..6760
                     /gene="fliF"
                     /locus_tag="VHE33_06895"
     CDS             5150..6760
                     /gene="fliF"
                     /locus_tag="VHE33_06895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HVT97218.1"
                     /translation="MADKADKPQSALTVQQRAGVLATEAATRFRNLDAGRRNRLLSAV
                     AIVLAGLGGLIWYASRPEWRTLYAGLDPDDARQVSQQLTTAGIPFDVSPDGSTLRVPA
                     PNLDKARLATTAKGGPRTGRMGFELFDNPNWMGSEFDEKVNYQRALEGELEHTIGTLA
                     SVQSARVHLVLPHDSLFSTDQREAKASVVLRLRHRTLSDEEAEAVGNLVASAVDGLRP
                     EKVVLVDAAGGGLLGRRGADAAGESREQEMAARLVETLEPAAGAGNVRASVTLEYDAN
                     SADELDETYDPAQTATLSMQRNEQNSGQPTASGIPGTASNAPNVKPPLYPAQSASQQS
                     TKQESATYGVSKKTRHTIEAAGKVRRLTAAVLINYRRTGEGGQVRWQPRTADEMKQLS
                     DLAQSAIGFDASRGDQVTLAELAFDGDAAQPEPTRIQRLLITAARGESLLRYGTILAA
                     VLLFLLFVARPVVRALTSTHVGKAAPAAALPLRIQAETEPAALPAERQLIEQRKTHAQ
                     SVFDQVAQTVKNDPVQSTRLLESWIRSE"
     gene            6817..7806
                     /gene="fliG"
                     /locus_tag="VHE33_06900"
     CDS             6817..7806
                     /gene="fliG"
                     /locus_tag="VHE33_06900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HVT97219.1"
                     /translation="MRKAAIFLVAVGDEIGKKILQQLAEADVQRLTEELAELRSIPPE
                     LSLEVVEEFSEMLDTQQFMMHGGLDYATKLLVDSFGKQRAEDLLALVKRAQEASQSDL
                     TVLQRVDPQQLGKFLESEHPQTIAVVLAHLDARKGSTVIDSLSEERRVEAIRRLAAMR
                     QFSPEMAQRVAMILHRRLENMGDTGRKAYSGFKAVADLLNRMESEEAKKILDEVEAAD
                     TAMALNIRNLMFTFEDLVTIPPASIREIVAAADKRQLALALRGAQEELRAHVFAAMSS
                     RAADMLKEDMEVMGPVRAREVAGAQGEILALARNLEAEGKVILKLEQGDDFLT"
     gene            7824..8525
                     /locus_tag="VHE33_06905"
     CDS             7824..8525
                     /locus_tag="VHE33_06905"
                     /inference="COORDINATES: protein motif:HMM:NF018358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HVT97220.1"
                     /translation="MSKNESEIRIMEYPALAGGRPVIKTQAPPDPVERVRELEAQLEM
                     SERRFAQGLENATRDALERGRGLAGAEQAALREQCVTQLKNAVEDFRSHRDEYFARVE
                     HEVVRLALAIAERILHREAQLDPLLLSGAVRVALGQLAEGTEVRLRVPAAQKEMWSEV
                     VRLMPGLPLRPEVRADESLREGEAALEAALGAVDLGVRAQLTEIERSFFDAHESVPDL
                     EGLEPDPAAAGGKRG"
     gene            8526..9863
                     /locus_tag="VHE33_06910"
     CDS             8526..9863
                     /locus_tag="VHE33_06910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HVT97221.1"
                     /translation="MELLGTYFTRLRQRPPYRWRGRVVEAAGQTIESEGPPCSVGESC
                     EIRDAAGNVHAAEVIGFHGTGVLSMTLGPADGIRYGDSVAALGTMPRVEVGEGLIGRV
                     MNATGQPLDGGRRPVLHSAVALERPAPAPFDREPIREALGTGIRAIDGLLSVGRGQRV
                     GIFGGSGVGKSTLIGMMTRNSEADVIVVGLVGERGREVGEFLEEGLGPEGKKRAVVLV
                     STSDQSALLRMRAALAATSVAEYFAGTGKHVLLVLDSLTRFAMAAREIGLAAGEPPTT
                     RGYTPSVFSRLARLVERAGRFRKGSITAFYTVLMEGDDLQDPLVDAVRSYLDGHVVLS
                     RSLASEGWYPPIDVLESISRLMPVVIDEAHARAAGVIRRLLASWARSEDLVRIGAYRA
                     GTDRDIDLALQFRPAIRDYLQQAARGHQSMADARNQLLRLAGAIEGSTPAGSP"
     gene            9860..10315
                     /locus_tag="VHE33_06915"
     CDS             9860..10315
                     /locus_tag="VHE33_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97222.1"
                     /translation="MNRSLRRLLRVRELVEDLAELDLETKNAWLRGLEAAAETQRSLA
                     RQGRADAVEMLLKGSPLQLESLPMQNADIQIAGMKEARLEALAESARVPVEQARDLLL
                     ERRRERRQAEILIDAAVSAEEKNRLRRDQNRTDDWFQSRSSPSRTRGQK"
     gene            10387..>10791
                     /locus_tag="VHE33_06920"
     CDS             10387..>10791
                     /locus_tag="VHE33_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97223.1"
                     /translation="MNCTVPMLPAPDAAKMPVADYAPAAPGAGGDLFGALVHLQMGSV
                     AARPLPIEVGIPTLQPANAPAIGTTVLLQSREMPANLLSQKNNLGEHTEEPPAGKLVG
                     GTNAARTSSRGKVEATPSGDCGSCLQTMTEPSA"
BASE COUNT         2042 a         2873 c         4045 g         1831 t
ORIGIN      
        1 gtatcagaag ggcaagttac agtttgacga cccggtggcg aagtatctgc cggcgttcgg
       61 tgccaacggg aagcaggaca ttacgattcg ggagctgttg acgcactatt cggggctacc
      121 gccagatcta ccgctggccg agccctggga gggacacggc acggcgtatc ggatggcgtt
      181 tgcgattgcg cctgcgggac cgccgggaga gaagtttgtc tacagcgaca tcaactttat
      241 tgtgctgggc gcgctggtcg aaaagctgtc agggatgacg ctgaatgagt acgcggagcg
      301 attcatcttt cagccgctgg gaatgcggca cacgcgtttt cttccgcccg agtcgtggat
      361 cccgcacatc gcgccgacgc agtacgagca tggggcctcg gcgtatggcg tggcagggct
      421 ggcgaccggt cccggcgacg tgatgctgcg cggggtggtg cacgatccca cgtcgcggcg
      481 catgggcggc gtggctggac atgccggggt gttcagcacg gcggacgatg tggcggtcta
      541 tgcgcagaat ttgctgaacc ggctggcggg gcggccgagc cggtttccgc tgtcgcgacg
      601 cgtcctgcag ctgatggtga agccggagca gccggccggg ggcaaggcgg tacgtggatt
      661 cgggtgggac atcgattcac cctactcgag caatcgcggt tcgatttttc cggtgggatc
      721 gtttgggcac acgggcttca cggggacgtc gctgtggatg gatccggcaa gcgatacgta
      781 cgtaattgtg ctggcgaatt cggtccatcc gaatgggccg aagcggatcg tcgccctgcg
      841 cggcaagatt gccgatgcgg cggcgagggc ggtgggagcg gagaagcacg cggcggccag
      901 tcgtcgctga gagccacctg cagaccgcgt gtgggttggt tcttgtttcg ggcctgaagg
      961 aaaccgcgga ttcctaatat ttacggagca cgccaaggac tctgccctgg atcgcgacct
     1021 gtgcggcggg cacgaggata gggtccattt cgatattggc aggctgcagc cggatcacag
     1081 agccttcgcg gtaatagcgc ttcagcgttg tttcggagcc gcggaccaga gcgaccacga
     1141 tttcgccgga gcgggcggtc gccgtgcgtt cgacgaggac gaagtcgccg tcgaggatgt
     1201 gctcatcgcg catggattcg ccgcggacct ggagagcgaa gacatcgcga ttgccgacga
     1261 tgtcggccag agagatgctt tccgggattt cggcggcttc gacgggatac ccagcggcga
     1321 tgcgcccggc cagagggagg cggtcgacgc cacggccttt ggaacggggc ggcaggacgt
     1381 cgagtgagcg gctgcgattg tgcgcgcggg ccagcaggcc tttgttctgc aggttggtga
     1441 cgtgcttgtg cacggtggcc agggagcgga ggtcgagacc gtgggcgatt tcttcgaagg
     1501 agggtgaata gccgtttcgc tgtacaaagc cggagatgaa ctcgaggact tccttctgcc
     1561 gccgggtgat cgccatagcg gccattatag cgaacaaaaa gcgaatggga agagaattcg
     1621 gcggtgctct ggctttccgg tttcccattc cggtacctgc gggcagggcg atttcattgc
     1681 gggaagggag tttggatttc tctcatattt cgattcacgg gaaggagaga aggagagaag
     1741 gtggggttgg gagaagcgac gatgcggttg ctgatagcgg aagacgatcc ggcgctgggc
     1801 aggtttctgg cccgcgggat ggaacaggat gggcacgagg tgacgatggc caccaatggc
     1861 gaaatggcga tggaggcgat gcggaacgat cttcctgaaa tggcggtgct cgatctgaat
     1921 cttccccgcc gggatggaac cgaggtgctg gagttcctgc gcacgctgac cgaggatgtg
     1981 ccggttctta tcctgacggc gcggaaagaa ccggaggtcc ggctgaggtg cctcgagatg
     2041 ggcgcggacg acttcatgca gaaaccattt tccttcgccg agctgcgcgc ccgctgccgt
     2101 gcgctgcaga gacggagatc cccgggactg gtgctgcggc agggggatgt ggagatgaaa
     2161 cgcgtggagc gtttggtgac gtgccagggg aaagcggtca ccctgaccac gcgggagttt
     2221 gcgctgctgg aatacctgct gctgcatcgc gggcgcgccg tttcgcgagc gactctgctc
     2281 cgggaggttt ggaacatgag cgaagcagcg ggaacgaatg tggtggatgt ctacatcaac
     2341 tatttgcggc ggaaactcga gagcgccggc gcgggcgccg ccgcgaatgg aagctgcgac
     2401 ggggaaacgc taatccggac ggtgcgtggc gtgggatacg cgatcggccg ggaaaccgga
     2461 acggcacctg cagccacggc tgtctaagga ggagagatgg gaagcatagc gcagtcgaga
     2521 cccggcagcg gaacccggac ggcggttctg gtgagtgcag atgcagcgtt cagggagcgg
     2581 ttgcggcgcg cgctttccga actgcggtgg caggtacggg agacggcggg cggagcggag
     2641 acgctggcgt gcctggaggc ttcgccggcc gagaccgtgg tggtggactc gtggctgccg
     2701 gacctggaga tccaggagtt cgtgacggag tttcggaagg tgcatccgga ggtcgacctg
     2761 gtgatggcgg atgcctcgga tgaagaccga agcgaggtgc ggggcccgcg gcgcaacgag
     2821 attttcctgg cgctgcgacg ggcgcaggcc accgatgacg cgatctggaa taccgccccg
     2881 cgcacgatcg atgccggagg ctgcgaggaa cgagtgacat ccggggaatc ccgcgtcctg
     2941 caagaggcgg gccaggctcc gccgacggtc cgggcggcag gggaattgcc ttccggtgcg
     3001 gcgatgcacg cgtctggttc tgtgccgtgg gccgccggat tgccggaatt tgtggggtcg
     3061 catccggcca tgctggaggt gagccggagg atccgactgg tggcgccgcg cgcgacgccg
     3121 gtgctgatcg aagggccaac cgggagtggc aaggagatgg tggcgcgtgc agtgcaccgg
     3181 ctgagcccga gggcgatgcg gcggatggta gcgctgaatt gcgcggcgat cccggaaggc
     3241 ctgctcgaag cggagttgtt cggacacacg cgtggcgcgt tcacgggcgc ggtcgacaat
     3301 cgcaccggac ggatcgaagc agcgcacggc gggacgctgt ttttggacga gatcggggag
     3361 atgcctttgg gcttgcaggc gaagctgctg cgtttcctcg agaccggaga actacaacgg
     3421 gtggggaaca acgaatcgat ccatgtcgac gtgagaatcg tggcggcgac ccatcgcccg
     3481 ctggggaagc tggtgaaagc gggaacgttt cgagccgacc tgtactaccg tctggcagta
     3541 ttcctgatcc gcacaccggc cctggcgggc cgggaccagg atgtgacggc gctgatcggg
     3601 cattttctca caaagatggg gaagagcagt ccggcgaagc agatgacgga ggcggcgctg
     3661 gtgcggctgg cacaacacac gtggcccggg aatgttcggg aactggaaca tgtcctggag
     3721 cgggcatgga ttctggccga agatcggccg cggatcgatc gggacgagat cgaattcgat
     3781 gaggagggat aagaggagtc tcatgattgc gaaggcaggg cgggggaaag ggtgcaggag
     3841 agggatggac ccgaaggacg aacgatgcag attctgacac cgatggcgga ggcgctgaac
     3901 cgatacctgg acctctcgtc gcaggagctg aagttgaccg cgcagaacat ggccaatgtc
     3961 gacacgccgg gataccggac cgtggggttc gattttgccg gggaaatgtc gcgttcgctg
     4021 gacggactac gaggaacggc gggcgccccg gcggcggatg ctcctgtgca tccggtgcgg
     4081 atcgatggac tgctggagcg tccggatggg aacaacgtgt ccatggaccg cgaaggaatg
     4141 aagatggccg aggcgcagct gcagttccag acgggagtgg aactgctgaa gcacgcgttc
     4201 tcacgtgtcg agaatgcgat tcgcatggat gagaagtaac ggcgcggggg cgaggaacgg
     4261 ttcgggctcg ggaagcggcc gctggttccg gacaggagga tgtatgttgc tgcccggcgc
     4321 gagagagttg aaagcagaga cagcaatcgg agcggaaagt gaggcgcgat gaatctgttc
     4381 ggggtgatgg aggtgagcgg atcggcgttg caggcggagc ggattcgggc cgaggtggtg
     4441 gccgcgaata tggcgaacgc cgagaccacg cgcaccgccg acggcgggcc ctatcggcgc
     4501 cagcatgtgg tgttcacgtc ggaagcggcg cagggcagtt tcggcgcgca tttgctggcc
     4561 aatccgggac aggcggacgg tgagatcggg ggcgtacgcg tgtcggcggt ggtggccgac
     4621 gagggggcac cgctgcggcg gtttgacccc gggcatcccg acgccgatca ggacggatat
     4681 gtgtcgtatc cggatatcaa tccgctgacc gagatggtgg atctgatggg ggcgacgcgg
     4741 gcctatgggc tgaacagctc ggcggtgcag gcggagaagg gcatgctcac cgcgtccctg
     4801 gaaattctta aatagaaggt tcgcgtatgc agacggctgg aatcggacat tcccttgcgg
     4861 cggtgctgac gacgccggcg gccccgatgg gatcggttgc gacggaagcg gcgcctttcg
     4921 gcgatctgct gaaaagtgcg attgcgggag tggatgggct cgaacaacag gcttcggcgg
     4981 ccgtggaagg gctgatgcgg ggaagcggag tggatctcca tgatgcactg atcgctacgc
     5041 agaaagcgga catgagcttt gagctggctc tggccgtacg caacaaagcg gtggccgcgt
     5101 atcagcaggt gatgggaatg cagttctgag cgcaggcgag tgagaccgga tggccgataa
     5161 agctgacaaa ccgcaatccg cgctgacggt gcagcagcga gccggagttc tggcgactga
     5221 ggcagcaacg cgcttccgca atctcgatgc gggacggcgg aaccgtctgc tgtcggcggt
     5281 ggcgattgtg ctggccgggc tgggagggct gatctggtac gcgtcgcggc cggaatggag
     5341 aaccttgtat gcgggcctcg atcccgacga tgctcgccag gtttcccagc aactgaccac
     5401 cgcgggaatc ccgttcgatg tttctccgga cggttcgaca ttgcgcgtac cggcgccgaa
     5461 tctggacaag gcgcggctgg ccacaaccgc caaagggggg cccaggaccg ggcgaatggg
     5521 gtttgaactc ttcgataacc cgaactggat gggatcggag ttcgacgaga aggtgaatta
     5581 ccagcgtgcg ctcgagggag agctggagca cacaattggg acgctggcgt cggtgcagtc
     5641 ggcccgggtg cacctggtgc tgccgcacga ttccctgttt tcaacggatc agcgggaggc
     5701 caaggcttcg gtggtcctgc gcctgcggca tcgcacgctc tccgatgagg aagctgaggc
     5761 tgtgggcaat ctggtcgcga gcgccgtgga cggtcttcgc ccggaaaaag tcgtgctggt
     5821 ggatgcggcg gggggagggc tgctgggacg ccgcggggcg gacgccgctg gggagtcgcg
     5881 cgaacaggag atggcggcga ggctggtgga aacgctggaa ccggcagccg gagcggggaa
     5941 tgtacgagct tcggtcactc tcgaatacga tgcgaactcc gccgatgaac ttgacgagac
     6001 ctacgacccg gcgcagacgg caaccttgtc catgcagcgc aatgaacaga attccggaca
     6061 gccgacggca tccggaatcc cgggcacggc gagcaatgca cccaatgtga agccgccact
     6121 gtatccggcg cagagtgcga gccagcagag cacgaaacag gagagcgcaa cgtacggtgt
     6181 ttcgaaaaag acccgtcaca cgattgaggc agcgggaaaa gtacggcgcc tgacagcggc
     6241 ggttctcatc aactatcggc gaaccgggga aggcgggcag gtgcgctggc aaccgcgcac
     6301 ggccgacgag atgaaacaac tgagcgacct ggcgcagtcg gcgatcggat tcgatgcctc
     6361 gcgcggggat caggtcacgc tggcggagct ggcctttgac ggagacgcgg cgcagccgga
     6421 gccaacccgg atccagaggc tgctgatcac agccgcgcgc ggggaatccc tgctgcgtta
     6481 cgggacaatt ctggccgcgg ttctgctctt tctcctcttc gtagcccgcc cggtggtccg
     6541 cgcgctgacg tcgacccatg tgggaaaagc tgctccagct gcggccctgc cgttgcgcat
     6601 ccaggcagag acggagccgg cggctttgcc ggcagagcgg cagctgatcg agcagagaaa
     6661 gacgcacgcg cagagcgtgt tcgaccaggt ggcgcaaacc gtcaaaaacg atccggtgca
     6721 aagtacgcgg ttgctggaaa gctggatacg gtcggagtaa ccatggcatc ccccgctgct
     6781 cgcccagtcg ccggttcagg tcacgcatta agcggagtgc gtaaagccgc gatttttctg
     6841 gtggctgtgg gagacgagat cggaaagaag atcctgcagc aactggctga ggcggatgtg
     6901 cagcggctga ccgaagaact cgccgaactg cgcagtatcc ctccggagct ttccctggaa
     6961 gtggtggaag aattcagcga gatgctggac acgcagcagt tcatgatgca tggggggctt
     7021 gactatgcca cgaagctgct ggtggacagc tttgggaaac agcgtgccga agacctgctg
     7081 gcgctggtga aacgggctca ggaagccagt cagagcgatc tgacggttct tcagcgtgtg
     7141 gatccgcagc aactggggaa gtttctggag agcgagcacc cccagacgat cgcggtcgtc
     7201 ctggctcatc tggatgcaag aaagggctct acggtcatcg acagcctcag cgaggaacgg
     7261 cgggtggagg cgattcggcg gctggccgcg atgcggcagt tttccccgga aatggcgcag
     7321 cgtgtcgcca tgattctgca tcggcggctg gagaacatgg gcgataccgg gcgcaaggcc
     7381 tactcgggat ttaaagcggt tgccgacctg ctgaaccgga tggagtccga agaggcgaaa
     7441 aagatcctgg acgaagtgga agctgcggat accgcgatgg cgctgaatat ccgcaatttg
     7501 atgttcacgt tcgaggatct ggtgacgatt ccgccggcca gtattcggga aatcgtagcg
     7561 gccgccgaca agcggcaact cgctttggcg ctgcgggggg cacaggagga gttgcgcgcg
     7621 catgtgttcg cagcgatgtc gtctcgcgcg gccgacatgc tgaaagagga tatggaagtg
     7681 atgggcccgg tgcgggcgcg ggaagtggcc ggcgcccagg gcgagatcct ggctctggcg
     7741 cggaacctcg aggccgaagg caaggtgatt ctgaagctgg aacagggcga tgattttctg
     7801 acctgagacg cggagtgggg ccggtgagca aaaacgagtc ggaaatccgg atcatggaat
     7861 atccggcgct ggccggcggg cgtccggtca tcaaaacgca ggcgcccccc gatccggtcg
     7921 agcgggtgcg cgaactggag gcgcagctcg agatgagcga acgccgattc gcccagggtc
     7981 tggagaacgc cacgcgggat gcactggaga gaggcagagg tctggcggga gccgaacagg
     8041 cggcgctgcg ggagcagtgt gttacgcagt tgaaaaatgc agtcgaggat tttcgttcgc
     8101 accgtgacga atactttgcg cgggtcgagc acgaggtggt gcgactggcg ctggcgatcg
     8161 cggagaggat cctgcaccgg gaggcacaat tggatccgtt gctgctctcc ggggcggtgc
     8221 gcgtggccct tgggcaactg gcggagggga cagaagtgcg gttgcgggta ccggcggcgc
     8281 aaaaggagat gtggtcggaa gtggtgcgcc tgatgccggg acttccgctg cggccggagg
     8341 tgcgggccga cgagagcctg cgcgagggcg aggcagccct ggaagccgct ttgggtgcgg
     8401 tcgatctggg cgtgagggcg cagctgactg aaatcgaacg cagcttcttc gatgctcatg
     8461 aatcagtgcc tgacctggaa ggcctcgaac ccgatccggc cgcagccggc ggaaagcgag
     8521 gatgagtgga actcctgggg acgtacttta cccggcttcg ccagcgcccg ccttaccggt
     8581 ggcgggggcg tgtcgtcgaa gcggccgggc agacgatcga atcggagggg cctccgtgtt
     8641 cggtcgggga atcctgcgaa atccgcgatg cggcggggaa tgtgcatgcc gcagaagtga
     8701 tcgggtttca cggcacggga gtgctgtcga tgacgctcgg tcccgccgat ggcattcgtt
     8761 acggagattc tgttgccgcg ttgggaacga tgccgcgcgt cgaagtggga gagggtctga
     8821 tcgggcgggt gatgaacgcg acgggtcaac cgctggacgg cgggcgaagg cccgttctgc
     8881 acagcgcggt ggcactggag agaccggcgc cggcgccgtt cgatcgcgag cctattcggg
     8941 aagcgctggg gacggggatt cgcgctatcg acggcctgct gagcgtggga cgcgggcaga
     9001 gggtcggcat ctttggcggc tcgggtgtgg gaaagagcac cctgatcggg atgatgacgc
     9061 ggaacagcga agccgatgtg atcgtggtcg gcctggtggg cgagcgcgga cgggaagtgg
     9121 gggaatttct ggaagagggg ctgggcccgg aggggaaaaa gcgcgctgtt gttctggtgt
     9181 cgacgtcgga tcagtcggcg ctgctgcgga tgcgggcagc cctggcggcg accagtgtgg
     9241 cggaatattt tgccggaacc gggaaacacg tcctgctggt tctggactcg ctgacgcgct
     9301 ttgcgatggc ggcacgggag atcgggctgg ctgcgggaga gcctcccacc acgcgcggct
     9361 atactccttc ggtcttcagc cggctggccc gcctggtaga gcgggcggga cggtttcgga
     9421 agggaagcat cacggcgttt tacacggtgc tgatggaggg agacgacctg caggatccgc
     9481 tggtcgacgc ggtgcgttcc tatctggacg ggcatgtcgt gctttcccgc tcgctggcct
     9541 ccgagggctg gtacccgccc atcgatgtgc tggagtcgat cagccggctg atgccggttg
     9601 tgattgacga ggcgcacgcg cgcgctgcgg gcgtcatccg gcgtttgctg gcgagctggg
     9661 cgcggtcgga agatctggtg aggatcggag cttaccgggc tgggacggac cgggacatcg
     9721 acctggctct gcaattccgg cccgccatcc gcgactatct gcaacaggcc gctcgcgggc
     9781 atcagagcat ggctgacgcg cgaaatcagc tgctgcggct ggcaggcgca atcgaaggct
     9841 cgactccggc aggatcgccg tgaatcgcag tctgcgccgt ttactgcgag tccgcgaact
     9901 ggtcgaggat ctggcggagc tggatctgga aacgaagaac gcatggctgc gcggtctgga
     9961 ggcagcagca gaaacgcagc gttcgctggc tcgacagggc cgtgccgatg cggtggaaat
    10021 gctgctcaaa ggatcgccct tgcagctgga atcgctgcca atgcagaacg cagacatcca
    10081 gatcgccggg atgaaagagg caagacttga agcgctggcc gagtcggcga gggtgccggt
    10141 tgagcaggcg cgtgaccttc tgctcgagcg gcgccgcgaa cgccgccagg ccgagatcct
    10201 gatcgatgcg gccgtgagcg cggaagagaa gaatcgattg cgtcgcgatc agaatcgcac
    10261 cgacgactgg tttcagagcc gctcctcgcc gagccgaaca cgaggacaaa aataaggcgc
    10321 ttcttagtcg ggcgatgccg ggattggcag gtccgatgca ggttcttccc gcgaggggat
    10381 cgccgattga actgcaccgt tcctatgctg cctgctcccg atgccgcgaa aatgccggtg
    10441 gcggactatg cgcctgccgc acccggggcg ggtggggact tgtttggcgc tctcgtccac
    10501 ctccagatgg gaagcgttgc tgcgaggcca ctcccgatcg aggtgggtat tccgaccctc
    10561 cagcctgcaa acgcgcctgc aatcgggacg acagttctgc tgcagagcag agaaatgccg
    10621 gcgaacctcc tgtcgcagaa gaacaacctc ggggagcata cggaggaacc gccggcgggc
    10681 aagcttgtgg gcggcacgaa tgctgcgcgg acttcgtcac ggggcaaggt cgaggcgact
    10741 ccgtcagggg actgcgggag ctgtctccaa accatgacgg agccttccgc a
//
LOCUS       DASZYN010000265         2906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_268331, whole genome shotgun sequence.
ACCESSION   DASZYN010000265 DASZYN010000000
VERSION     DASZYN010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268331"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VHE33_06925"
     CDS             complement(<1..262)
                     /locus_tag="VHE33_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97224.1"
                     /translation="MRFSLRFVPGLLFVSLTFMVHGFASAQNASEPSAADQDRQSMMD
                     QLGIQALRPGWSGNEKAPNHANYDESKANPYPNLPDPLTMNDG"
     gene            334..1587
                     /locus_tag="VHE33_06930"
     CDS             334..1587
                     /locus_tag="VHE33_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HVT97225.1"
                     /translation="MMNCARGIPIFLWVVAAFVLVASYAQARSSAPNWILTWGASQQI
                     PEPHNALPSADLRDATVRQIFHLSVGGSTIRVHVTNTFGAAPLHFTSVHIAQPLAADA
                     PAIDPASDRALSFAGASDVTVPAGADVVSDPIAFAVAPLSNLAVTWHLDDPPAPETSH
                     PGSRATTYFVHGDQVSAATFQNPGTIEHWYQVSAIDVQAPAGAGVIAAFGDSITDGHA
                     TTTNGNDRWTDVLAARLQQSAATKNIGVLNDGIGGNHLLTDGLGPNALARFDRDVLAP
                     SGVRWLIVFEAINDLGGLTRLHEVPQADHDALVQRIIGAYQQMIDRAHAHGIRVFGAT
                     ITPDGGSDYYHPGSLNEADRKAVNDWIRAPGHFDAVLDFDAVVRDPQQPDRMLPACDS
                     GDHLHPGPAGYRAIGNSIPLSLFAH"
     gene            complement(1597..>2906)
                     /locus_tag="VHE33_06935"
     CDS             complement(1597..>2906)
                     /locus_tag="VHE33_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HVT97226.1"
                     /translation="GDMLPSSPEARLAARSNRTGASLLKRLATKLPHQWNGIRHVDLA
                     ELQLATSETARIINRDIVLELIRASQPISRADLARRSGLGRSTISQIVEQLIRENWVR
                     EGAMGSPPRGRRPTLVGLNENLVAIAVDIHPRQASVALVDLNGRLLSRWPVPLTSDPG
                     ASMRLILESMQRMQRSAERKSVEGVGISLPGRVNPVTHRLIFAPNLKWPDFDIKKMVE
                     TKMGLPVRMENDATACLVAELTFARMDGVRDAVLVAVAEGIGTCVLTNGELVSGYNGM
                     AGEFGHVPIDPNGPRCGCGKKGCWEMFGSTRAALRNYRDLRPKSHVATFQELLNLAEE
                     GDAAAAQALALQAQGIGRGLRMIIAGLSPSTILIAGDVTPAWHRFGPVIEKEAIDLTL
                     AGSPPVIRPALEGEIARLRGAAALVFQRGKRFGTEPATAAQSA"
BASE COUNT          502 a         1041 c          818 g          545 t
ORIGIN      
        1 ggccgtcgtt catcgtgagt gggtcaggaa gattcgggta cggattcgcc ttcgattcgt
       61 catagttcgc gtgattcggc gccttctcgt tgccactcca gccgggccgc agcgcctgga
      121 tgccaagctg atccatcatg ctctggcgat cctgatccgc cgcactcggt tcgctcgcat
      181 tctgcgcgct tgcgaatccg tgaaccatga aggtgagtga cacgaagagc agaccgggta
      241 caaacctgag agagaagcgc acgcgtcctc cgcttgctgg tattctggct ggcgttcgag
      301 ccgaaatgat tcgggaccga tactaaatcc tccatgatga attgcgcacg cggaattccc
      361 attttcctgt gggtggtggc ggcctttgtc cttgtcgcga gctacgctca ggctcgaagc
      421 agcgccccga actggatcct cacctggggg gcctcgcagc aaatccccga gccgcacaac
      481 gcattgcctt ccgccgatct gcgcgacgcc acggtccgcc agatctttca cctctccgtt
      541 ggcggttcga cgatccgcgt gcacgtcacg aatacgtttg gcgccgcgcc gctgcacttc
      601 acgtccgtac atattgcgca gccgctcgcg gccgacgctc cagcgatcga tccagccagc
      661 gaccgcgcgc tgagctttgc cggcgccagc gacgtcactg tccccgccgg agccgacgtc
      721 gtctccgatc cgattgcatt cgccgttgct ccgctctcga acctcgccgt cacctggcac
      781 ctcgacgatc cgcccgcgcc ggagaccagc caccctggct cgcgtgcgac cacctacttc
      841 gtccacggcg atcaggtcag tgctgcgacc ttccagaatc ccggcaccat cgaacactgg
      901 tatcaggttt ccgcaattga tgtgcaggcc ccggccggcg ccggcgtgat cgctgctttc
      961 ggcgactcca tcaccgacgg ccacgcgacc accaccaacg gcaatgatcg ctggacggac
     1021 gtgctggccg cgcgccttca gcagtccgct gcgacaaaaa acatcggggt actcaacgac
     1081 ggcatcggcg gcaaccacct cctcaccgac ggcctcgggc ccaacgcttt ggcccgcttc
     1141 gatcgcgatg tgctcgcgcc cagcggcgtt cgctggctca tcgtctttga ggcgatcaac
     1201 gatctcggcg gtctgacgcg cctccacgaa gtgccacagg ccgatcacga tgcgctggtg
     1261 cagcgcatca tcggcgcgta tcagcaaatg atcgatcgtg cgcacgccca cggcattcgc
     1321 gtcttcggcg ccaccattac ccccgacggc ggctcagact actaccatcc aggctcgctg
     1381 aacgaggccg atcgcaaagc cgtcaatgac tggattcgcg cgccgggcca cttcgatgcg
     1441 gtcctcgact tcgatgctgt ggtccgtgat ccgcagcaac ccgaccgcat gctgcctgcc
     1501 tgtgacagcg gcgaccatct gcacccgggc ccggcaggct accgcgccat cggcaactcg
     1561 attcccctct cgctctttgc gcactgaacg caggattcaa gcggactggg cagcggtcgc
     1621 cggctccgta ccgaatcgct tgccgcgctg gaacaccagc gcagccgcgc cccgcagccg
     1681 cgcaatctcg ccctcgagag cgggccggat caccggcggg ctcccggcca gtgtcaggtc
     1741 aatcgcttct ttctcgatca ccgggccaaa ccgatgccac gccggggtca cgtcgccggc
     1801 aatcaggatc gtgctcggcg acaggcccgc aatgatcatg cgcagccctc gcccgattcc
     1861 ctgcgcctgc agcgccaacg cctgtgctgc cgccgcgtcg ccttcctcag ccagattcag
     1921 cagctcctgg aatgttgcta catgcgattt cggccgcagg tcgcggtaat tacgcaatgc
     1981 cgcgcgcgta gagccgaaca tctcccagca gcccttctta ccgcatccgc accgcggccc
     2041 gttcggatcg atcggcacgt gcccgaattc ccccgccatg ccgttgtagc ccgacaccag
     2101 ctccccgttg gtcagcacac aggttccaat accctctgca acggccacca gaaccgcgtc
     2161 ccgcactccg tccatgcgcg caaaagttaa ctcggccaca aggcatgccg tcgcgtcatt
     2221 ctccatccgc accggcaggc ccatcttcgt ctcgaccatc ttcttgatgt cgaagtccgg
     2281 ccatttcagg ttgggcgcaa agatcagacg atgggttacc ggattcaccc ttcccggcag
     2341 gctgatgccc acgccttcca ccgatttccg ctccgccgat cgctgcatcc gctgcatgga
     2401 ctccagaatc agccgcatcg acgctcccgg atcggacgtc aggggcacgg gccatcgcga
     2461 cagcagccgc ccattcagat ccaccagggc gacgcttgcc tgccggggat gaatgtcgac
     2521 ggcgatggcg accaggtttt cgttcagccc caccagggtc ggacgccgtc cccggggcgg
     2581 cgagcccatc gcgccttcgc gaacccagtt ctcccggatc agttgctcca cgatttgcga
     2641 gattgtgctt ctgcccaggc cggaacgccg tgccagatct gcccgggaaa tgggctggct
     2701 ggcgcgaatc agctccagca caatatcgcg gttgatgatg cgcgctgttt cgctcgtggc
     2761 cagctgcaat tcagccagat cgacgtgtcg gatgccattc cactggtgcg gcaattttgt
     2821 cgccaacctc ttcaacaacg atgcgcctgt ccgattcgat cttgcggcaa ggcgcgcttc
     2881 tggggaagaa ggaagcatat cacccg
//
LOCUS       DASZYN010000266         1762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_74927, whole genome shotgun sequence.
ACCESSION   DASZYN010000266 DASZYN010000000
VERSION     DASZYN010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74927"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1762
                     /locus_tag="VHE33_06940"
     CDS             <1..>1762
                     /locus_tag="VHE33_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97227.1"
                     /translation="QDTSFKQTIDQKEVNELPLNGRQMTDLIVLSGGAASAPGNDMVG
                     SKNFFSSKSISVAGGMGNQTDYRLDGADNNDYMTNVNLPFPFPDAVNQFSVESTAMGA
                     QSGLHPGGEVNVVTNSGTNAFHGDAFEYVRNNLINATNFFSSRKDQLHQNQFGGTLGG
                     PVLRDKLFFFAGYQRTVHHEVTSDSTAFVPSNANLAGDFSATDSTPLYNPITGALLAG
                     NHIDPATYFSPVSLALEKFLPKTDDPTGKVLYAPPGDYTENQFVSRVDANLTSKNNLY
                     GRYFLDGYALPAKWDPTNALVTATPGNLERVQSLTIGDSQVFSAHTVNTVHATVDRRR
                     DNRTPAAEGVSPNAIGVNIFQIDPNFFRFTVSSKFALYCGTCAPSHFNVNTFSIADDV
                     NLVRGRHQIVFGGEFARSQFNASNHYEENGAFTFSGIFSQTGPAQNQPKQAKADGNLD
                     FLTGALQAFEQSKAQGNSLRAPIPTLYVQDTWQATPKLVLTAGVRWQPEYMPADYFHR
                     GSVYSESSFLGSEHSTVYPNSPAGVFYYGEKGVPKGFTQNSPWQFSPRVGVTWNPDGS
                     GKTVFRLGGALIYDEPNFFTA"
BASE COUNT          381 a          562 c          456 g          363 t
ORIGIN      
        1 gcaggacacc tccttcaagc agacgattga ccaaaaagag gtcaatgagt tgcccctcaa
       61 tggccgccag atgaccgacc tgatcgtgct ttctggcgga gctgcgagtg ctcccggtaa
      121 cgatatggtc ggcagtaaga atttcttcag ttccaaatcc atttcggtcg ccggcggcat
      181 gggcaaccaa accgattatc ggctggatgg cgccgacaac aacgactaca tgaccaatgt
      241 taatcttccg tttcccttcc ccgacgcggt caaccagttc agcgtcgaga gcacggccat
      301 gggcgcgcag agcggtctcc atcccggcgg cgaggtcaac gtagtcacga actctggaac
      361 gaatgccttc catggggacg ccttcgagta cgtccgcaac aacttgatca acgcgacgaa
      421 ctttttctcc agcaggaagg atcagcttca ccagaatcag ttcggcggca ccctcggcgg
      481 tccggtcctg cgcgacaagc tcttcttctt tgccgggtac caacgaacag tccaccacga
      541 ggtcacttcg gactcgaccg cgtttgtccc gagtaatgcc aatcttgctg gcgatttctc
      601 ggccactgac tctacacctc tctacaatcc gatcactgga gccttgctgg ccgggaatca
      661 cattgaccct gccacgtatt tcagccctgt ctcgctggca ctggaaaagt tcctgcccaa
      721 gaccgacgat ccaaccggaa aagtcctgta cgctccgccc ggtgactata ccgaaaatca
      781 gttcgtgagc cgggtcgatg ccaatctgac gtcaaaaaac aacctctatg gccggtattt
      841 cctcgatggc tacgcgcttc cggcaaaatg ggacccaaca aacgcgctcg taactgcgac
      901 gcctggcaac ctcgaacgcg tccagtcatt gacgattggc gatagccagg tttttagcgc
      961 gcatacagtc aacaccgtgc acgccaccgt cgaccgccgc cgcgacaacc gcacgccggc
     1021 cgccgaggga gtcagcccga acgcaattgg cgtgaatatt tttcaaattg accccaattt
     1081 cttccgcttc acggttagtt cgaaattcgc cctctactgc ggcacctgcg ctccctctca
     1141 tttcaacgtg aacacgttct ccattgcaga cgacgtgaat ctggtccgag gacgacacca
     1201 gatcgtcttc ggcggtgagt tcgcgcgttc tcagttcaac gccagcaatc actacgagga
     1261 gaatggagca ttcaccttca gcggcatctt cagccagact gggcctgccc agaaccagcc
     1321 caagcaggcg aaggcagacg gaaatcttga tttcctcacc ggagcactgc aggccttcga
     1381 gcagagcaag gcccagggca attccctgcg cgccccgatt ccgactctgt acgtccagga
     1441 cacttggcag gccaccccga agctggtgct cacggccgga gttcgctggc agccggagta
     1501 catgccggcg gactatttcc accgcggctc cgtatacagc gaatcttctt tcctgggcag
     1561 tgagcacagc acggtctatc cgaattcgcc ggcgggcgtc ttctactacg gcgagaaagg
     1621 ggtgcccaag ggcttcacgc agaactcacc ctggcagttt tcgccccgcg tcggagtgac
     1681 ctggaacccc gatggttccg gcaagacggt cttccgcctt ggcggtgcgc ttatttatga
     1741 cgagcctaat ttctttacgg ct
//
LOCUS       DASZYN010000267         1912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_74965, whole genome shotgun sequence.
ACCESSION   DASZYN010000267 DASZYN010000000
VERSION     DASZYN010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74965"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VHE33_06945"
     CDS             complement(<1..823)
                     /locus_tag="VHE33_06945"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97228.1"
                     /translation="MNRRQFIEQGSIAAGALALRGSRAFAAPAEEGDASSAFRTEDAG
                     LQHIYDAALATLAGNAVTFPSYPGPLLVEGSVYHGVWLECAPQEGEVYEAVGPEAARQ
                     LARDNHLIFFALQKEDGQLPCSVKEGGPAYGQIQMQVPIAATAWELAQRTGDQELLEK
                     AYAACGRWDEWLRRYRDTRGTGLCEGFCTYDTGQDNSPRWKGLANRCPDSDARKCPDE
                     PGMPRLCPDLSATVYGGRVALAAMAKALGKTDDASRWKEDAETIQGMIIEKLYDPV"
     gene            944..>1912
                     /locus_tag="VHE33_06950"
     CDS             944..>1912
                     /locus_tag="VHE33_06950"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT97229.1"
                     /translation="MILRLLLDFGIFGLCTSTISAILALVGLGRFLSQRRAPASVFTA
                     PVSLLKPLHGDEPDLEAHLASFFDQDYPAFEIIFCARSASDPGLAAARRVAARFPHIP
                     AQFLSTGEQTYINAKVSSMERMHAAAQHDIFIISDSDVRVTRNYIREVVAPFADAEVG
                     AVTCLYRGVAQRGLWAKLEAAGMSVEMTSGVLTANLLEGMKFALGPTMAVRRRSVEAM
                     GGFGVLGPFCSDDFLLGEKVAAQGQTVVLSHHVIDHIILNLSVAASLRHQTRWMKSTR
                     FSRPLGHLGTALTFSLPFGILACLAAMTLHRPILGFLLLAYSIANRM"
BASE COUNT          298 a          752 c          476 g          386 t
ORIGIN      
        1 ccaccgggtc gtagagcttc tcgatgatca tgccctggat cgtctcagca tcttccttcc
       61 atcggcttgc atcgtcggtc ttgcccagcg ccttggccat cgcagccagc gcgacgcgtc
      121 cgccatacac cgtcgccgaa agatcgggac acagccgcgg catccccggt tcgtccgggc
      181 acttgcgcgc atcgctgtcc gggcaccggt tcgccagtcc tttccagcgc ggactattgt
      241 cctgccccgt atcgtaggtg cagaagcctt cgcacaaacc cgtgccgcgc gtgtcgcgat
      301 accgccgcag ccattcgtcc cagcgcccgc acgccgcgta cgccttctcc agcagctcct
      361 gatcgcccgt cctctgcgcc agttcccacg ccgtcgccgc aatcggcacc tgcatctgga
      421 tctgcccata cgccggcccg ccctccttca cgctgcacgg caactgccca tcctccttct
      481 gcaacgcaaa gaagatcagg tgattgtcgc gcgcaagctg ccgtgccgct tccggcccga
      541 ccgcttcgta cacctcccct tcctgcggtg cacattccag ccatacgccg tgatagacac
      601 ttccctccac cagcaacggg cccggatacg acgggaacgt caccgcattt ccggccagcg
      661 tcgccagcgc tgcgtcgtag atatgctgca atccggcgtc ttccgtccga aacgcgctgg
      721 acgcgtcccc ctcttcggcc ggcgccgcaa acgcccgcga cccgcgcagc gcaagcgctc
      781 ccgcagcgat cgatccctgt tcaatgaact gacggcgatt catcgcctct ccgctgtccc
      841 gcccaaccag ggtatcaccc ccatctctac tccagccctg tatgagatag cctcataaag
      901 gcagccgcgg agcgcagctt ctggagtgcc gcatgccgcg cctatgatcc ttcgactgct
      961 cctcgacttc ggcatcttcg ggctttgcac ctcgacgatc tccgcgatcc tcgccctcgt
     1021 cggccttggg cgctttcttt cacagcgacg cgcccccgcc tccgtgttca cggcgcctgt
     1081 ctcactgctc aagccccttc acggagacga gcccgacctc gaggctcacc tcgcctcctt
     1141 cttcgaccag gattaccccg ctttcgagat catcttctgc gctcgttccg cttccgatcc
     1201 cggtctggcc gccgcacgcc gtgtcgcagc gcgctttccg cacattcccg cgcaattcct
     1261 ctccaccggc gagcagacct acatcaatgc gaaggtctcc tcaatggaac ggatgcacgc
     1321 cgccgcgcag cacgacatct tcatcatcag cgacagcgat gttcgcgtca cccgcaacta
     1381 catccgcgaa gtcgtcgcgc cgtttgccga tgcggaggtc ggcgccgtta cctgtctcta
     1441 tcgtggcgtc gcccagcgcg gcctctgggc gaaactcgaa gccgccggca tgtcggtcga
     1501 gatgacctcc ggcgtcctga ccgcaaacct tctggaaggc atgaagttcg ccctcggccc
     1561 caccatggcc gtccgccgcc gctccgtcga agccatgggc ggattcggcg tccttggtcc
     1621 cttttgctcc gatgattttc ttctcggcga aaaggtggca gctcagggcc aaacggtcgt
     1681 cctttcccat cacgtcatcg atcacatcat cctcaacctc agcgttgccg cctccctgcg
     1741 ccatcaaacg cgctggatga aatccacccg cttctcgcgc cccctcggcc atctcggcac
     1801 cgcactcacc ttcagccttc ccttcggcat cctcgcctgc cttgcagcaa tgacgctgca
     1861 ccggcccatc ctcgggttct tactcctcgc gtactccatc gcgaatcgta tg
//
LOCUS       DASZYN010000268         8794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_778248, whole genome shotgun sequence.
ACCESSION   DASZYN010000268 DASZYN010000000
VERSION     DASZYN010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8794
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778248"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VHE33_06955"
     CDS             complement(<1..883)
                     /locus_tag="VHE33_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97230.1"
                     /translation="MTAHSFRSTSLYLLVAFSSALLAQAPAKIDPTHMAKLGTVDPRY
                     LSYNVEMVEVTGGRFWRPYASLAQAAKDKPAPSATQPGDMSALYEYRPPLDLSNERLR
                     KLAAALGPAYIRVSGTWQNSTYFQNDDQPALAKPPEGFRSVLTRAEWKGVIDFARVAD
                     ARIVTSFAVSPGTRNADGVWQPEQAQALLDYTRAAGGTIAAAEFMNEPNFPSAGGAPS
                     GYDAAQYGRDARIFGVFLHKQSPHTIFLGPGSVGEGITLMPAGSHMPQITFLHSEDLL
                     QATGPIFDAFSYHFYGAV"
     gene            complement(1071..1613)
                     /locus_tag="VHE33_06960"
     CDS             complement(1071..1613)
                     /locus_tag="VHE33_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97231.1"
                     /translation="MKPALKVVFILLAYTCTLSVRAQCGFFSKPITGFNWYRAEGWPT
                     PGVDDAKSIVPLTINRKGMPLIIDGRDVNWPEGITVSRLTHEKDYHLVFPSVIFQEDG
                     QRKQMQPAGFSLVALLRWEVHGKPYALTYSVLSDTVACGFSMDLIDDVGDGKFRQMQS
                     SNRMTIGFPPLPVWANRPTS"
     gene            complement(1670..4123)
                     /locus_tag="VHE33_06965"
     CDS             complement(1670..4123)
                     /locus_tag="VHE33_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HVT97232.1"
                     /translation="MKRTISHYELERKLGAGGSGVVWLANDTQLMRPVVLKILKQGAL
                     TLDQMRTTVLREARLASAIEHPNVCAIYEVDEEGEEAFIVMQYVPGQSLDKLIARGPA
                     TLQLVLSVGIQIADGLFAAHSLGIFHRDLKPANVMLTDGGLAKILDFGLARRLPPEER
                     DFDPAKSSRRKPGAPIATYTARGGTIAYMAPEQFVTGQSSVQSDLWGFGVILYELASG
                     RHPFARPDGDEFQSIRAIQFADPLPLPDNIPPELTSVVVRLLQKIPAERYVSAADVRE
                     ALKTIMKALQLESGIIPGDAAAVLPPSTPAEQEKRTTGILSMLAERFRESTEEKSARN
                     TILVLPFQNLGAPSTAPLYGFALADGIATRIARMSALIVRPSSALMHLPIAQMDPLAI
                     GQKLFVDWVLTGNFMRSDKGFDLNWQLLEVDSQSVRGGGSISVASFDLIAVQTEIVNE
                     VFASLQGIGQLQPGANHQMSSPRPPAVLGEELSEEYLQAQALLSGFMQRTGSRSDLER
                     ARQLFEAVTRRDASFAPAFTGLGIAELQYVRYGFGGHLHVIAARKALDRALELDPNSV
                     DANLYRVYMLLSRGEKESARHGIEHLLRTAANDWNVHLVAGITLRIDGMYDEALAQFH
                     RALMLNPASAAVVYNHRARVYQYQNQLELAEEELQKGLTLEPRYPLLRTSLAYQRMRQ
                     GDLHQAIHMLEQVIRDDASMRLAVPTLALCYVMMDDRQRAASLIVEETIAAAEADSEM
                     SYRLATYFAVDGDESEALHWLRRAIYLGNENYPWFSKNPAWNRLKEHTDFERILDDLR
                     KSYRRNQKTWKRLLSESGG"
     gene            complement(4248..6683)
                     /locus_tag="VHE33_06970"
     CDS             complement(4248..6683)
                     /locus_tag="VHE33_06970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT97233.1"
                     /translation="MESILQDVRYALRKLAKSPGFALIALLTLSLGVGAATAIFSVVD
                     TVILRPLPFSQPDRIFVPQTVSGQGYTQPFSLPSFKDFRAQNHVFAAVSAVSSYQGVN
                     LQTPSGPVALNWVQGSDNFFDVFGVAPLLGRTYRPGEDQPGNSDVAVLSYDVWQTNFS
                     GDPGVIGRAVELNGHPYTCIGVMPAGFRYPLSVRHAIYTPLHPDPLVVENRGDHWLPA
                     IGRLKPGVTRQQAQADLSAVIANIGRAFPATDGGRKVQVISLADRVVGNVTGALWTLS
                     AAVLAVLLIACVNVAGLLLARGVKREREVALRAAIGAHRSRLLRQELTESLLLAGLGA
                     AGGILLSWLLLAALRVFLVHALSRGADVHIDIPVLFAALALSIGTSIAASLYPALHLS
                     GAQPSQALRSGGTGGTSRAHHRLRSVFIVSQVALSLVLLVVAGVLLREVAGYRNTDVG
                     FDTRHILAAEIDLSPERWQNHNVWADFYQPMLDRVSHLPGVRAAGVINLVPIQNWGWN
                     SEIHITGQPPTPPNELTLTEDRYVSPGYFDALGIRLLQGRMLSPSLDVPTNKAAAIVV
                     NQAFVKKFLPAGVNPAGQHIDDSDKADEKTQIVGEVTGVRQDLMQPSMPESDYLYSGV
                     PEKYSSILMRSNLIVRTSGDPRSVIPALRNAFRQIDPTLPFREPQTMDEIIADQLVMQ
                     RMETWLFGIFASLAVLLAVVGLYGLISHEVELGTRDIGIRMALGATRRGVFALILRRV
                     AILLGIGIVTGLALTVAARRLIASVATLHFADQWGLLALLVLILGAAGLLAAVLPMQR
                     AASIQPMEALRTE"
     gene            complement(6906..7400)
                     /locus_tag="VHE33_06975"
     CDS             complement(6906..7400)
                     /locus_tag="VHE33_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97234.1"
                     /translation="MTPTDRDLSGLEWLSRMSRWLDPGQDPVVTSSAPAAVLLQKGPA
                     PAPSVEPSILPMMSTELQPMKIASKIESSLSEIPWTRTVAAGSLVVGALLLIAGRRKS
                     ALAVSAAGAAVALLENPEIVRETWDAMPRVIRSGQDFLVRIEDFVEELNKQGQHIKKV
                     LVQD"
     gene            complement(7415..7729)
                     /locus_tag="VHE33_06980"
     CDS             complement(7415..7729)
                     /locus_tag="VHE33_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97235.1"
                     /translation="MAKSQSNSTFEIACPDCGAMLKIDPSTRAVIAHTPAPRKRTFED
                     FDQATRALREAEERKESIFRQSVEAHKNQEDVFNKRFEEALRKAKETPDTGKPLREFD
                     LD"
     gene            complement(7875..8384)
                     /locus_tag="VHE33_06985"
     CDS             complement(7875..8384)
                     /locus_tag="VHE33_06985"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HVT97236.1"
                     /translation="MTDSTVSEIERMVVTRILDAPRELVWKAWTDPRYVMQWWGPKGF
                     TAPVCRMDVRVGGKSLLCMKTPDGYEGWNAIEYHEIVLHEKIVSSMYFSDSEGNKIDP
                     AALGIEQEAIEGAYDVTLFEDLGNGRTKLTFIGNEPMASAKDSGQWEGWNETFDKIAA
                     VVAELAQAK"
     gene            complement(8374..>8794)
                     /locus_tag="VHE33_06990"
     CDS             complement(8374..>8794)
                     /locus_tag="VHE33_06990"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVT97237.1"
                     /translation="TCRDHKQKSGIAKFTLDMDEIIHNHMVVDYLDRTFAALSDPTRR
                     AMIERLSRGPASVHGLTEPFALSQQMISKHIACLVRARIVTKTKRGRESVCTLRPGAI
                     KTVSDWAISYRRFWEESFDKLEVVVNQMKKEEVRDDG"
BASE COUNT         1710 a         2447 c         2970 g         1667 t
ORIGIN      
        1 atacggcccc gtagaagtga taggagaagg cgtcgaagat cggtcccgtc gcctggagca
       61 gatcctcgga gtgcaggaag gtgatctgag gcatatgcga gccggcaggc atgagcgtta
      121 tgccttcacc gacacttcct ggccccagaa aaatcgtatg cggcgactgt ttgtgcagga
      181 agacgccaaa gattcttgcg tcacggccgt actgggcagc gtcataccca gagggcgcgc
      241 ctcccgcgga gggaaagttt ggctcgttca tgaactcagc cgccgcgatc gttccgccgg
      301 ccgctcgggt gtagtcgagc aacgcctggg cctgttccgg ctgccagact ccatcggcat
      361 tgcgcgtgcc cggactcacg gcaaacgagg tgacgatgcg ggcatccgcg acgcgcgcaa
      421 aatcgatcac gcccttccac tctgcgcgcg tgagcacgct gcggaaaccc tccggaggct
      481 tcgcgagggc gggttgatcg tcattctgaa aatacgtgct gttttgccag gtaccgctca
      541 cgcggatgta ggccggtccc agggccgcgg ccagtttgcg cagacgctcg ttgctcaggt
      601 cgagcggggg acgatattcg taaagggcag acatatcccc cggctgcgtg gcgctgggcg
      661 caggcttgtc cttcgccgcc tgcgccagcg aggcgtacgg cctccagaaa cggcctccgg
      721 tcacctcgac catttcgacg ttgtaggaga gatagcgcgg gtcaacggtg ccgagtttgg
      781 ccatgtgggt ggggtcgatc ttcgcgggcg cctgagcgag gagcgcagac gagaatgcta
      841 cgaggaggta gagcgacgtg cttcggaacg agtgagctgt catgttgtgc cggtgccata
      901 gtacacgggt agttggggga ttgcgtccgt gctctggtgt ttttggggtt ggaggtcgct
      961 catgaacgaa gtcgatcttt cggggcgtgt ttccctggcc tgaaatctga agccttttcc
     1021 gcgtactgtt ttgcggcgcc acgtgaccgc tgcattgcga tggtgagaaa tcaggatgtt
     1081 ggcctgttgg cccataccgg aaggggcgga aagccaatgg tcatacgatt cgagctttgc
     1141 atctgacgga atttgccgtc tcccacatcg tcgatcaaat ccatcgagaa cccgcaagcg
     1201 actgtatccg aaagaactga ataggtgagc gcataaggct tcccgtgaac ctcccaacgc
     1261 aaaagggcaa ccaacgagaa gcccgccggc tgcatctgtt tgcgttggcc atcctcctga
     1321 aagattaccg atggaaacac gaggtgataa tccttctcat gcgtgagtcg cgagacggtt
     1381 ataccctcgg gccagttaac atcgcggcca tcgatgatga gaggcatccc cttcctgttt
     1441 atcgttagag ggacaataga ttttgcgtca tcaacgcccg gcgttggcca gccctcggcg
     1501 cgataccaat tgaatcctgt gatcggtttg ctaaagaaac cgcattgcgc ccgaaccgac
     1561 aaagtgcagg tgtacgccag caaaatgaaa acgactttga gcgcgggttt catgagctct
     1621 acaactcggc tgtaaattgt atcgctgaac agattccgtg cgttgtcgat tacccacccg
     1681 actcgctgag gagccgcttc cacgtcttct gattccggcg gtagcttttt ctcaggtcgt
     1741 cgaggatgcg ctcgaaatcc gtgtgctctt tgaggcggtt ccaggcgggg ttcttcgaga
     1801 accagggata gttctcgttg cctaaataaa tcgcgcggcg gagccagtgg agcgcttcgc
     1861 tctcatcgcc atcgacggcg aagtacgtcg ccaggcggta ggacatctcg gagtcggctt
     1921 cggcggcggc aatggtctct tccacgatga gcgaggccgc gcgctggcgg tcgtccatca
     1981 tgacatagca aagagcaagc gttgggacag caaggcgcat cgaggcgtcg tcgcgaatga
     2041 cctgctcgag catgtggatg gcctggtgga gatcgccctg gcgcatgcgc tggtaggcga
     2101 gagatgttct cagcagcggg tagcgcggct ccagggtcaa tcccttctgg agttcttcct
     2161 cggcgagctc cagctggttc tggtactggt agacgcgcgc tcggtggttg tagaccacgg
     2221 cggcgctggc cggattgagc atgagagcgc gatggaactg ggcgagcgcc tcgtcgtaca
     2281 tgccgtcgat gcggagcgtg atgccggcga cgaggtgaac gttccagtca ttggcggcgg
     2341 tgcggagcag gtgctcgatg ccgtggcgcg cggattcctt ttcgccgcgc gacagcaaca
     2401 tgtaaacccg gtagaggttg gcgtcgaccg agttgggatc gagctcgagg gcccggtcga
     2461 gggctttgcg cgcggcgatg acgtggaggt gccccccgaa tccgtagcgg acgtactgca
     2521 actctgcaat gccgaggccg gtaaaggcgg gcgcgaaaga ggcgtcgcgc cgggtgacgg
     2581 cttcgaagag ctggcgggca cgttcaaggt cggagcggga accggtgcgc tgcatgaatc
     2641 cggagagcag ggcctgggcc tggaggtatt cctcggagag ctcttcgccc agaaccgcgg
     2701 gcggccgcgg gctggacatc tggtggttgg cgccgggctg gagctggccg atgccctgca
     2761 gcgatgcgaa aacttcgttg acgatttcgg tctggacggc gatcaggtcg aatgacgcaa
     2821 cgctgatgga gccacccccg cgcacggact gcgagtccac ttcgagaagc tgccagttga
     2881 gatcgaagcc tttgtcggaa cgcatgaagt tgccggtgag gacccagtcg acgaagagct
     2941 tctggccgat ggcgaggggg tccatttgcg cgatgggcag gtgcatgagc gccgatgatg
     3001 gacgcacgat gagcgcggac atgcgggcga tgcgcgtggc aatgccgtcg gccagggcga
     3061 agccgtagag cggcgcggtc gagggagcgc ccaggttctg gaagggcagc acgaggatgg
     3121 tgttgcgcgc ggacttctct tcggtggact cgcggaagcg ctcggcgagc atggagagaa
     3181 tgccggtggt gcgcttttcc tgctcagccg gcgtggaagg cggaagaacg gcagcggcgt
     3241 caccggggat gatgcccgac tccagctgca gcgccttcat gatggtcttg agggcttcgc
     3301 ggacatcggc agcggagacg taccgttcgg ccgggatctt ctgcaggagg cggacgacga
     3361 ccgacgtcag ctcgggggga atgttgtcgg ggagcggcag cgggtcggcg aactgaatgg
     3421 cgcggatgga ctggaactcg tcgccgtcgg ggcgggcgaa gggatggcgg ccgctggcga
     3481 gctcatacag gatcacgccg aagccccaga gatcggactg gacggagctt tggccggtga
     3541 cgaactgctc gggcgccata taggcgatgg tgccgccgcg cgcagtatag gtggcgatgg
     3601 gtgcgcctgg ttttcgccgc gaggacttcg cgggatcgaa gtcgcgctct tcggggggca
     3661 ggcgccgggc gaggccgaag tcgagaatct tggcgaggcc gccatcggtg agcatgacgt
     3721 tggccggctt caggtcgcga tggaagatgc cgagcgagtg agcagcgaag aggccgtcgg
     3781 caatctgaat accgacggag aggacgagtt gaagcgtcgc cggaccgcgg gcgatgagct
     3841 tgtcgagcga ctgtcccggc acgtactgca tcacgatgaa ggcttcttcg ccttcctcgt
     3901 cgacttcgta aatggcgcag acattggggt gctcgatggc ggaggcgagg cgggcctcgc
     3961 gcagcacggt ggtgcgcatc tggtcgaggg tgagcgcgcc ctgcttgagg attttgagga
     4021 cgacggggcg catgagctgg gtgtcgttgg cgagccagac gacgccggaa ccgccagcgc
     4081 cgagcttgcg ctccagctcg tagtgggaga tggtgcgttt cattgggagc ctggttttag
     4141 tgtagcggcg tgccctgtga ggatttctgc aggtttccca gcggcttctg agacgcacgc
     4201 cgatgcgccg tcgggagccg cctgctcgtg gacgaagatt attgccctta ctcggttctc
     4261 agggcctcca tgggctgaat ggaagctgcg cgctgcatgg gcaagaccgc ggcgagaagt
     4321 ccggcggcgc caaggatcag aacgaggagg gcgagcagac cccactggtc tgcgaagtgg
     4381 agcgtggcaa cggaggcgat gagccgcctg gcggccacag tgagagccag gccggtgacg
     4441 atgccaatgc ccagaaggat cgcaacgcga cgcaggatga gggcgaacac ccctctgcgc
     4501 gtggcgccga gcgccatgcg gatgccgata tcgcgcgtgc cgagttccac ttcgtggctg
     4561 atgagcccgt agagaccgac gacggccagc aggacggcga gcgaggcaaa aatgccaaag
     4621 agccaggtct ccatgcgctg catcacgagc tgatcggcga tgatctcatc catcgtttgc
     4681 ggttcgcgga acggcagtgt gggatcgatc tgccggaagg cattgcggag cgcgggaatc
     4741 acggacctgg gatcgcccga agtgcgcacg atgagattgg agcgcatcaa aatcgagctg
     4801 tacttctccg ggaccccgct atagagatag tccgactccg gcatcgaagg ctgcatcaga
     4861 tcctggcgga cgccggtgac ctcgcccacg atctgcgttt tctcgtcggc tttgtcgctg
     4921 tcatcgatgt gctggccggc aggattgact cccgcaggga ggaatttctt cacgaaggcc
     4981 tgattcacga cgatggccgc tgccttgttc gtcgggacat cgaggctggg cgaaagcatg
     5041 cgtccctgca gcaggcgtat gcccagtgca tcgaagtagc ccggcgagac atagcgatcc
     5101 tcggtcaggg tgagctcgtt cggcggcgtg ggcggctggc cggtgatgtg gatctcggag
     5161 ttccagcccc agttctgaat gggaacgagg ttgatgactc cggcggcgcg cacaccgggc
     5221 agatggctga cgcggtccag catgggctgg tagaagtcgg cccagacatt gtggttctgc
     5281 cagcgctcgg gcgagagatc gatctccgca gcgagaatgt ggcgggtatc gaatccgacg
     5341 tcggtattgc ggtaaccggc gacctcgcgc agcagcaccc cggcaaccac cagcagaacc
     5401 agcgaaagcg cgacctgcga gacgatgaag accgagcgca gacgatgatg agcgcgcgaa
     5461 gttccgcccg tgccgccgct gcgcaatgcc tggctgggct gagcgccgga aaggtggagc
     5521 gcgggataca gcgaggcagc aatgctggta ccgatggaga gcgccagggc ggcgaacagc
     5581 acgggaatat cgatgtgaac atcggcgccg cgcgagagag cgtggacgag gaacacgcgc
     5641 agggccgcaa gcaacaacca ggaaagaaga atgccgccgg cggcgcccag acccgcgagc
     5701 agcagacttt cggtcagttc ctgccgcagg agccgcgaac ggtgcgctcc gatggcggcg
     5761 cgcagggcga cctcgcgctc gcgtttgacg ccgcgagcca gcagcagtcc cgcgacattg
     5821 acgcaggcga tcagcaaaac ggcgagcacg gccgccgaga gcgtccacaa cgcgccggtg
     5881 acattgccca cgacgcggtc cgccaggctg atgacctgga ctttgcgacc gccatcggta
     5941 gcgggaaagg cgcggccgat gttggcgata acggcgctca ggtcggcctg cgcctgctga
     6001 cgcgtcactc caggtttgag ccggccgatg gcgggcagcc agtggtcgcc tcgattctcc
     6061 acgaccagcg ggtctggatg caggggcgtg taaatcgcat gccgcacgct cagcggatag
     6121 cggaaccccg cgggcatgac accgatgcag gtatagggat gcccgttcag ttcgacggcg
     6181 cggccgatca cgccgggatc gccgctaaag ttggtctgcc acacgtcgta gctgagcacg
     6241 gcgacatcgc tgttgccggg ctggtcttcg cccgggcggt aggtgcggcc gagcagaggc
     6301 gcaacaccga acacatcgaa gaaattgtcc gagccctgaa cccagttcag agcgaccgga
     6361 ccggagggag tctgcagatt gacgccctga taggacgaaa ctgcggagac tgcggcaaag
     6421 acgtggttct gggcacgaaa gtctttgaaa ctgggcagcg aaaacggctg ggtgtagccc
     6481 tgtccggaga cggtttgcgg cacgaagatg cgatcgggct ggctgaatgg cagcgggcgc
     6541 aggatcacgg tatcgacgac gctgaagatc gcggtggccg caccgacccc gagcgaaagc
     6601 gtgagcaaag cgatcagggc gaacccgggc gacttggcca gcttgcgaag ggcgtagcga
     6661 acatcctgga ggatggattc catggagatc tccggcgggg cggaatctgg ggaaggccgc
     6721 agaattagtg aagcacggat caggccagga aaggtgttgc aggacgcggc tcatccctaa
     6781 agctttgaaa gaagagagag catttgtttc gcatcaaggg tgttcggcgg cggtcagaac
     6841 tgttcgggga cggacagcgg gcgaacgcat cgcacgcttg cccgcccttg tgaaccggta
     6901 gcccgttagt cctgaacgag gactttttta atatgctgac cctgcttgtt cagttcctcg
     6961 acgaaatctt caatacggac gagaaaatcc tgcccggaac gaatgacgcg gggcatggcg
     7021 tcccaagtct cccgcacgat ctccgggttt tcgagcaggg cgacggccgc cccggcggcg
     7081 gagaccgcca gagctgactt gcggcggccg gcaatgagca ggagagcgcc gacgaccagc
     7141 gaaccggcgg cgacggtacg ggtccagggg atttccgaga ggctggattc gatcttgctg
     7201 gcaattttca tgggctgcaa ttcggtagac atcataggta agatgctcgg ctcaacagaa
     7261 ggagcagggg ccggtccttt ctgtaatagt actgcggcgg gtgcggagga cgtgacaaca
     7321 gggtcctgac cggggtcgag ccagcgagac atgcgggaga gccattcgag tccggagaga
     7381 tcgcgatcgg taggggtcaa cttgctcctg atggctaatc gaggtcgaat tcgcggaggg
     7441 gtttgccggt atcgggggtc tctttcgctt tcctgagggc ttcttcgaag cgcttgttaa
     7501 agacgtcttc ctggttcttg tgtgcttcaa cggattggcg gaagatgctt tccttgcgct
     7561 cttcggcttc gcgcagggcg cgggtggcct ggtcgaagtc ctcgaatgtg cgtttgcggg
     7621 gggccggtgt atgagcgatg acagcgcggg tggagggatc gattttgagc atcgcgccgc
     7681 agtcggggca ggcaatctca aaggtcgaat tggactggga tttggccata cgactctaca
     7741 aagcgtgagt gtaacgcagg ggagcgggga gggtacaggg tgtattcggc gaagcgacgg
     7801 aagcccgttc taaccgaacc tccgccgcgc cttcgctttc tctcctggat gacgatcgtc
     7861 gagacgtcaa cttcctattt cgcctgcgcc agctctgcca caacggccgc aattttatcg
     7921 aatgtctcgt tccagccttc ccattgcccg ctatcctttg cgctcgccat gggttcattt
     7981 ccgatgaagg tcaatttcgt cctgccgtta ccgagatcct caaagagagt cacgtcgtac
     8041 gccccttcga tggcttcctg ttctattccc aaggctgcag gatcaatctt gtttccttcc
     8101 gagtccgaaa agtacatgga ggaaacgatc ttttcgtgca gaacaatctc gtggtattca
     8161 atcgcattcc agccctcgta cccatccggc gtcttcatgc agaggagaga ttttcctcca
     8221 acgcgaacat ccatccggca aacgggcgca gtaaagccct tcggtcccca ccactgcatc
     8281 acgtacctgg ggtctgtcca cgccttccag accaactcgc gtggggcatc gagaatcctt
     8341 gtaacgacca tccgctcaat ttcgctcacc gtgctatccg tcatctctga cctcctcttt
     8401 tttcatttga ttaacaacca cttccagttt gtcgaagctc tcttcccaga accggcgata
     8461 gctaatcgcc cagtcgctga cggtcttgat cgcccctggc ctgagcgtgc acacactttc
     8521 tcgtccacgc ttcgtcttgg tcacgatccg cgcccgcacc aggcaggcga tatgttttga
     8581 aatcatctgc tgcgaaagtg caaagggttc cgttaatcca tgcacagagg caggcccacg
     8641 ggagagccgt tcgatcatgg cccggcgggt tggatcagac agagccgcaa atgtcctatc
     8701 cagataatcc acaaccatat ggttgtggat tatctcgtcc atgtcaagtg tgaatttggc
     8761 gattcctgat ttctgtttgt gatctcggca agtg
//
LOCUS       DASZYN010000269         5460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_845698, whole genome shotgun sequence.
ACCESSION   DASZYN010000269 DASZYN010000000
VERSION     DASZYN010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845698"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..1994)
                     /locus_tag="VHE33_06995"
     CDS             complement(261..1994)
                     /locus_tag="VHE33_06995"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97238.1"
                     /translation="MLRLLLCAALSCSLLPTTAQAAGPLDPHLVASKWPASWISSPSA
                     SPRAPGVFYFRRELTLTGVPAHYWVHVTADNRFILHVNGKYAGEGPARGDLFHWRFET
                     IDLAPLLHSGSNILAAVVWNFGEAAPVAQMTNRTGFLMQGDTEAESAVNTGSNWRVRQ
                     EAGRSELGHAGTHEYYAAGPAEKIDGRTLDWTWDQPGDDSAWETPRLIGRAATREAQD
                     ADNNWELVQDPLPPMEHRLVDAGVPVRADGLPSLPDFPAAPLEIPAHSHVTLLLDHRV
                     LQTAYPELTLSGGRDATIALTYSEALYDAHNNKGNRDEIAGRHIEGITDQFIADGGDQ
                     HAFQPLWWRTWRYLQLDITTQDAPLRLDSLRAWFSAYPFDAKAAISGDIPELDRLWQT
                     GWRTARLCAHETYMDAPYWEQLQYVGDTRIQALISYAMTGDSRLARQAITNIDDSRTP
                     EGITQSRYPSGLPQFIPPFSLLWVGMVHDYWMYVDDPALVKETLPHTRTVLDWFASHL
                     RPDGLLGKVGWWEFGDWTADYPDGVPPQEADGGSTFLTLQFMEALGYAADMESRYGSP
                     ERANQYRAIIR"
     gene            complement(2061..3347)
                     /locus_tag="VHE33_07000"
     CDS             complement(2061..3347)
                     /locus_tag="VHE33_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97239.1"
                     /translation="MTLLRPAREVWVPATAGDRHAVLSELQKILASPHFCNSKRYPAL
                     LEYIVEHALSGDVERLKERTLGIEVFHRPPDYDTNADTIVRYTAGEVRKRLALYYHEN
                     NNGDGIQIVLPVGSYVPEFLRAAPEESTGEQPSPATHPVAEPKFAAALHEEPPTPSPS
                     IVAAPEKQEDPGEHPRRRSKWWLSIAALLVLACGLVAVLRWRSSPTPEISAVNSFWSP
                     FFHEPGTALICSGAVVFAPEKFSGVTTANRDNQYPFVSIQMASAIARLSGLLESNHVA
                     YQMQPCTSIPLNDLRDRPLILLGGYNNDWTMRLVEPLRFTFIPGQEESISDRRNPSVR
                     WQRTPDQPYSSADDYGLIARFHDSTTDSMVIIIAGIGRNGTEAAGLFSTSDHYMGLLQ
                     KKLGSSLASGNIEAVIKVSVVDGKTGAPALEDAVRW"
     gene            3551..4759
                     /locus_tag="VHE33_07005"
     CDS             3551..4759
                     /locus_tag="VHE33_07005"
                     /inference="COORDINATES: protein motif:HMM:NF017263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HVT97240.1"
                     /translation="MDLHVSISRRLFCVGGAAAWAATAYGREARRRGFDLVSKTDRRR
                     VLTAAAEYVRLEPKTITSFPTKRSPGGLHDFFSQADYFWPNPANPDGPYINRDGQSNP
                     DNFNEHRKAMIALSIQMPALTAAWILTKKREYAVKAADHLRAWFVTPATRMNPNLEYS
                     QGVHGVSTGRSYGIIDTLHLAEVARAASLVAPAALDAGERTALLAWFRDYLHWMETSE
                     KGIAERDTKNNHAVCWALQASEYARLIGDAETRKAVRDRYKDVFVPDQMGHDGSFPRE
                     LARTKPYGYSIFNFDMMAMVCQSLVDGGEDLFEFALADGRGICLAAAFLEPYLKDKSA
                     WPYRKDVQHFDAWPVRSPGLLFCGLHCHRDNYIDVWKGLNPDPKDPEIIRNYPVRQPL
                     LWFQEAQAAA"
     gene            4756..>5460
                     /locus_tag="VHE33_07010"
     CDS             4756..>5460
                     /locus_tag="VHE33_07010"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97241.1"
                     /translation="MSGFDGGNEGTHTGGHLSRRTFVKGGMALAAAAMVNMPAMASVL
                     QSAGAAGQGSEPLSQRLDNGWEYYRGPLDPRFQVWHSDEIVTWDKVTLPHCFNHYDAC
                     DPDVPAYRGQGWYRTQLAVANPFSDGRTLLHFEGAGQQAEVWIGNTLAGKHVGGYDEF
                     LVDVTDACRALPAGQPLPLGVLCDNGRDIDRLPSDLSDFTLYGGLYRAVHLVYVPAIS
                     LEAVHTRVAFEMGKPAG"
BASE COUNT          984 a         1408 c         2020 g         1048 t
ORIGIN      
        1 tccagggcgc ggctcaaata gaagcgaaaa taatacgtcg gggcggagag ggcgggcacc
       61 tggcggccat cgacggtggt gggctggtcg gtcttcgagg ccagcagacg gtgcaggatc
      121 ggcttctgct cggccggcgg cgcgacgtcg agccacacgg cgaggatgtt tgcctcgtgg
      181 ctgtaggtct tcttcgcggg cgtgtcggcg tagaggccgt agtgccggtc ccagtttttc
      241 tgattgagcg attgtgcagc ttagcggatg atggcgcgat attgattcgc acgctcggga
      301 ctgccgtagc gtgattccat gtccgcggcg tatccgagcg cttccatgaa ctgtagggtg
      361 aggaaggtgg aaccgccgtc ggcttcctgg ggcggcacac catcaggata gtcagcggtc
      421 cagtcgccaa actcccacca gccgactttg ccgagaaggc catcggggcg caggtgggag
      481 gcgaaccagt cgaggacggt gcgggtgtgg ggcagagttt ctttcacaag cgccggatcg
      541 tcgacgtaca tccagtagtc gtgcaccatg ccgacccaca gcaaggagaa gggaggaatg
      601 aactggggaa ggccggaggg atagcggctc tgggtgatgc cttcgggggt gcgcgaatcg
      661 tcgatgttgg tgatggcctg gcgcgcgagg cggctgtcgc cggtcatcgc gtaggagatg
      721 agggcctgga tgcgggtgtc gccgacatac tggagttgct cccagtaggg cgcatccata
      781 taggtttcgt gcgcgcagag gcgggcggtg cgccatccgg tttgccagag gcgatcgagt
      841 tcagggatgt cgccgctgat ggcggctttg gcgtcgaatg gataagcgga gaaccaggca
      901 cgcagggaat cgagacggag cggtgcgtcc tgcgtggtga tgtcgagctg caggtagcgc
      961 caggtgcgcc accagagggg ctgaaaggcg tgttggtctc cgccatcggc gatgaactgg
     1021 tcggtgatgc cctcgatgtg gcgtccggcg atttcatcgc gattcccttt gttgttatga
     1081 gcgtcgtaca gcgcctcgga gtaggtgagg gcgatggtgg cgtcgcgtcc gccgctgagg
     1141 gtgagctccg ggtatgcggt ttggaggacg cgatggtcga ggagcagcgt cacatggctg
     1201 tgcgcgggga tctccaaagg cgcggcaggg aaatcgggga gcgacggcaa tccgtcggcg
     1261 cgcactggga cgccggcatc gacgagacgg tgctccatgg ggggcaatgg atcctggacg
     1321 agctcccagt tgttgtcggc atcctgggct tcgcgcgtgg cggcgcgacc gatgaggcgc
     1381 ggcgtttccc aggcggagtc gtcgccaggc tggtcccagg tccagtcgag agtgcggccg
     1441 tcgatttttt cggcgggacc ggctgcgtag tattcgtgcg tgccggcgtg acccagctcg
     1501 gagcggcctg cttcctggcg aacgcgccag ttgctgccgg tgttgactgc ggattcggct
     1561 tcggtgtcgc cctgcatgag gaagccggtg cggttggtca tttgcgcgac cggagctgct
     1621 tcgccgaagt tccaaacgac ggctgcaagg atgttggagc cggaatgcag caggggggcg
     1681 aggtcgatgg tttcgaatcg ccagtggaag agatcgccgc gggcgggccc ttcgccggcg
     1741 tatttgccat tgacgtggag gatgaagcga ttgtcggccg tgacgtggac ccagtaatgc
     1801 gcgggaacgc cggtgagcgt gagctcgcgg cggaaataga aaactcctgg ggctcgggga
     1861 gatgccgagg ggctgctgat ccaggacgcc ggccactttg aggcgacgag gtgagggtcg
     1921 agcggacctg cggcctgtgc ggtggtggga agaagggagc aactcaacgc ggcgcagaga
     1981 aggagacgca gcatggccga caggttaaca gaggagagag ggtagaattg gcgccgcgcg
     2041 tccgggatgc cggctgctgt ctaccagcgc acggcgtctt cgagggcagg cgcgccggtc
     2101 ttgccgtcga cgacgctcac cttaatgacg gcttcaatgt tgccgctggc gaggctcgag
     2161 ccgagctttt tttgcagcaa gcccatgtag tgatcgctgg tactgaaaag accggcggct
     2221 tcagtcccgt ttcgtccgat accggcaatg atgatgacca tgctgtcggt ggtggagtcg
     2281 tggaagcgcg cgatgaggcc atagtcgtcg gcgctggagt agggctggtc cggagtgcgc
     2341 tgccagcgga cgctgggatt cctgcgatcg ctgatgcttt cctcctgccc aggaatgaag
     2401 gtgaagcgca gcggttccac gaggcgcatg gtccagtcgt tgttgtaacc gccgaggaga
     2461 atcaaaggcc ggtcgcggag atcattgagc gggatggagg tgcagggctg catctggtac
     2521 gcgacgtggt tgctttccag aaggccgctg agccgggcga tggccgacgc catctggatg
     2581 gagacgaagg ggtactggtt atctctgttg gcggtggtga cgccggagaa tttctccgga
     2641 gcgaagacga cggcgcccga gcagatcaac gcggtacccg gctcatgaaa aaacggagac
     2701 caaaagctgt tgacggcact gatttcgggc gttggggagg agcgccagcg cagcaccgcg
     2761 acgagaccgc aagcgaggac gagcagggca gcgattgaca gccaccattt cgagcggcgg
     2821 cgagggtgtt cgccggggtc ctcttgcttc tccggggcgg caacgatcga tggggagggc
     2881 gtgggtggtt cttcgtggag tgcggcggcg aattttggct cggcgaccgg atgcgtcgcc
     2941 ggcgacggct gctcgcctgt cgattcttcg ggagcggccc ggagaaattc agggacatag
     3001 gatccgacgg gcagaacgat ctggattccg tcgccgttgt tgttctcgtg gtagtaaagg
     3061 gccagccgtt tgcggacttc gccggcggtg tagcgaacga tggtgtcggc gttggtgtcg
     3121 taatcgggcg ggcggtggaa aacctcgatg ccgagcgtgc gttctttgag acgttcgacg
     3181 tcgccggaaa gggcgtgctc cacgatgtat tccagcaggg cggggtagcg cttgctgttg
     3241 cagaagtgag ggctggcgag gatcttctgc aattcagaaa ggacggcatg gcgatcccct
     3301 gccgtcgcgg ggacccagac ctcccgtgct ggacggagca atgtcatcgg ggcgcaacaa
     3361 tcgatgatac tcggttgcga aaaggcccgc agcaacgttt gtaacgtcgc tgttgctccc
     3421 cgtaactgat tgacgttggg gaggttgcag acggggttcg gagccaaacg gtgagtcact
     3481 tgcatgggct tgtaacgtgc gctgaggatg cacaggtttg cgaggagcag ggcgcgaacg
     3541 actcactcga atggacttgc acgtctcaat ttccagaaga ttgttttgtg tcggcggggc
     3601 agctgcctgg gcggcgacag cctatgggcg ggaagcgcgg cggcgcggct tcgacctggt
     3661 gtcgaaaacc gatcgccggc gggttctgac agcggcggcg gaatatgtcc ggctggaacc
     3721 gaagacgatc acgagctttc cgacgaagcg tagtcctggc gggctgcacg actttttctc
     3781 ccaggcggac tatttctggc cgaacccggc gaatccggat ggtccgtaca tcaatcggga
     3841 cgggcagagc aacccggaca atttcaacga gcaccgcaag gcgatgattg cgctgtcgat
     3901 ccagatgccg gcgctgacgg cggcctggat tctgacgaag aagcgggaat atgcggtgaa
     3961 ggcagcggat catctgcggg cctggttcgt tacgccggcg acgcgcatga atccaaatct
     4021 cgaatattcg caaggcgtgc acggggtttc gacggggcga tcgtacggga tcatcgacac
     4081 gctgcatctg gcggaagtgg cgcgggcggc gagcctggtg gctccagctg ctctcgatgc
     4141 aggggagagg accgcactgc tggcgtggtt tcgcgactac ctccactgga tggagaccag
     4201 cgagaagggc attgcggagc gggatacgaa gaacaatcac gcggtgtgct gggctttgca
     4261 ggcgagcgag tatgcgcggc tgatcggcga cgcagaaaca cgcaaagcgg tgcgcgatcg
     4321 gtacaaggat gtttttgtcc cggatcagat ggggcatgac ggcagttttc cgcgggagct
     4381 ggcgcgaacg aagccgtacg ggtattcgat cttcaacttc gacatgatgg cgatggtgtg
     4441 ccagtcattg gtggatgggg gagaggacct gtttgagttt gcgttggcgg acgggcgggg
     4501 gatttgcctt gcggcggcct tcctcgagcc gtatctcaaa gacaagagcg cgtggcccta
     4561 tcggaaggac gtgcagcatt tcgacgcatg gccggtgcga tcgccggggc tgttgttctg
     4621 cgggcttcat tgccaccgcg acaactacat cgacgtttgg aaagggctga accctgatcc
     4681 gaaggatccg gagatcatcc ggaattatcc ggtgcggcag ccgctgctgt ggtttcagga
     4741 agcgcaggcc gcagcatgag cggatttgac ggcggcaacg aaggaacaca taccggaggg
     4801 cacctttcgc ggcgcacgtt tgtgaagggc ggcatggcgc tggcggcggc tgcgatggtg
     4861 aacatgccgg cgatggcatc ggtgctgcag agcgctggag ccgccgggca gggaagcgag
     4921 ccgctgagcc agcgcctgga caatggctgg gaatattatc gcgggccgct cgatccgcga
     4981 tttcaggttt ggcacagcga cgagatcgtc acgtgggaca aagtgacgct gccgcattgc
     5041 ttcaaccact acgatgcgtg cgacccggat gtgccggcgt atcgggggca ggggtggtat
     5101 cggacgcagc ttgcggttgc caatcctttt tcagacgggc ggacgctgct gcattttgaa
     5161 ggtgcggggc aacaggccga ggtatggatt gggaacactc ttgctggaaa gcatgtgggc
     5221 ggctacgacg aatttctcgt cgatgtgacc gacgcatgcc gggctttgcc ggccggacag
     5281 cccttgccgc tgggcgtgct ttgcgataac gggcgcgata tcgatcgcct gccgtcggac
     5341 ctgagcgatt tcaccttata tggcgggctt tatcgcgccg tccatcttgt ttatgttccg
     5401 gcaatttcgc tggaggcggt tcatacgcgg gtggctttcg aaatggggaa gcccgcgggc
//
LOCUS       DASZYN010000270         1856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_461675, whole genome shotgun sequence.
ACCESSION   DASZYN010000270 DASZYN010000000
VERSION     DASZYN010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461675"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1856)
                     /locus_tag="VHE33_07015"
     CDS             complement(<1..>1856)
                     /locus_tag="VHE33_07015"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HVT97242.1"
                     /translation="WITCLVFFQTALLLGYLYAHWLGKLSSARAATVVHLALLVAAAG
                     LLIVVGRPDLSQASSHPQSSIFAVLTLTIGLPFLMLASTSPLLQLWLAREERSGVPWK
                     LFALSNAGSLLALALYPTAIEPYLSLSVQRKLWGCGFGVYAIVCAVIARRMRGTDGGE
                     VALRAKPTSPTAGIWGTPRMEGSSLRARLLWFLLPAVGAIQLCAVTSHLSQNIAAIPL
                     LWVLPLGVYLLTFIIAFEAPPLRYQWIIARLLIVLLAALAWMLSRTDVTIPIQVSIGF
                     FLAELFFACLFLHVETYARRPENPSESTAFYLLIAAGGAAGTIVIGILCPMVFDANYD
                     VALAFLATAAAATLVMWDKGWGQRLLWGTGTGLLLALVVMLHIVYARNAVVEERNFYG
                     ALRVKESHVPPGAETVRTLMHGTIEHGTQWFADGFRRNPTTYYATDSGVGLALRFCCD
                     DRARNIGVVGLGAGTLAAYGKQGDRIRFYEINPLVRPIAENLFTYLRESGAQISFANG
                     DARISLTQEGSQHFNVLVIDAFSGDAIPVHLLTTQAMAVYREQIAPGGILAFHVSNQY
                     LDLTPEVAALAGASGMEARVVDTPAQESRGEYRATWVLVSNNAAFFGQPEVAA"
BASE COUNT          424 a          683 c          463 g          286 t
ORIGIN      
        1 gccgccacct ccggctgccc gaagaacgca gcgttgttcg acaccagcac ccacgtcgcc
       61 cggtattcgc cgcgcgactc ctgtgccggt gtatccacca cgcgcgcttc cattcccgac
      121 gccccagcca gtgccgccac ctccggcgtg agatcgagat attggttcga tacgtgaaac
      181 gccagaatcc cacccggtgc gatctgctcc cgatacacag ccatcgcctg cgtcgtcagc
      241 aagtgcacgg ggatcgcgtc gccggaaaac gcatcgatca ccagcacatt gaaatgctgc
      301 gacccctcct gcgtcagcga gattcgcgcg tcgccattcg caaacgaaat ttgcgcgccc
      361 gattcgcgca gatacgtaaa caagttctcc gcaatcggac gcaccagcgg gttgatctcg
      421 taaaaccgta ttcgatcgcc ctgcttgcca taggctgcca gcgttcccgc tcccagcccg
      481 accactccga tgttgcgcgc ccgatcgtcg caacaaaacc gtaatgccag ccccacgccc
      541 gaatccgtcg cgtaataggt cgtcgggttc cgccgaaatc cgtccgcgaa ccactgcgtc
      601 ccgtgctcga tcgttccgtg catcagcgtt cgcaccgtct ccgcccccgg cggtacatgg
      661 gattccttca cccgcagcgc tccgtaaaag ttccgttcct ccacgaccgc attgcgcgca
      721 tacacgatgt gcagcatcac caccagtgcc agcaggagcc ctgtacccgt gccccacagc
      781 aaccgctgcc cccaaccctt atcccacatg acaagagtgg cggccgctgc agtcgccaga
      841 aacgccagcg ccacgtcgta attcgcatcg aagaccatcg gacagaggat cccgataaca
      901 atcgtccccg ccgctccccc cgccgcaatc agcaaataga aagccgtcga ctccgacgga
      961 ttctccggtc gccgtgcata cgtttccaca tgcaggaaaa ggcacgcaaa gaacaattcc
     1021 gccaggaaaa acccgatgct gacctggatc gggattgtca cgtctgtcct cgacagcatc
     1081 cacgccaggg ccgccagcaa cacgatcaac aaccgcgcaa tgatccactg ataccgcaac
     1141 ggcggcgcct caaacgcgat gatgaacgtc agcaggtaga cacccagcgg cagcacccac
     1201 agcagcggaa tcgctgcgat gttctgactc aggtgactgg tcacggcgca aagctgaatc
     1261 gccccaaccg cgggcagcag gaaccacagc agcctcgctc gcagtgaact cccctccata
     1321 cgcggggtgc cccatatccc tgcagttggg gatgtgggtt tcgcgcgaag cgcgacctca
     1381 cctccatccg ttccacgcat ccgccgcgcg atcaccgcac aaacgatcgc gtaaacccca
     1441 aacccacatc cccacaactt ccgctgcaca ctcagcgaca aatacggctc gatcgccgtc
     1501 ggatacaaag ccagagccag cagcgacccg gcattcgaca gcgcaaacag cttccacggg
     1561 acgccacttc gctcttcgcg cgccagccaa agctgcagca aagggctcgt cgaagccagc
     1621 atcagaaacg gaagcccgat cgtcagcgtc agcacggcaa aaatcgaact ctgcggatga
     1681 ctcgaggcct ggctcagatc cggccggcca actacgatca gcaaccccgc agccgcaacc
     1741 agcagcgcca gatgcacaac cgttgccgct cgtgcggacg agagcttccc cagccagtgc
     1801 gcgtacaaat atcccagcag cagcgccgtc tgaaagaaca ccaggcacgt gatcca
//
LOCUS       DASZYN010000271         3188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_652242, whole genome shotgun sequence.
ACCESSION   DASZYN010000271 DASZYN010000000
VERSION     DASZYN010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652242"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="VHE33_07020"
     CDS             <1..1352
                     /locus_tag="VHE33_07020"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT97243.1"
                     /translation="GHRAVYNCQSLMSENAPTASAHAPHRRWRIAVLLGIGVLVNFFD
                     RVNVSVAFTSLHAEWGISAVTFGYLASAYNWSYGALQVPIGVMLDRWGVRRIGRVAAF
                     LWSAASIASGLATGVYSFFAARLLLGIGEAPTFPANAKAVGYWFPEHERSFATSLNDA
                     AAKFASAIGVPILGVFVISLGWRWSFLFTGFISFLYFLLFWRVYRNPSEDPRLSPAER
                     QLLVEGKAQPEIAPEHRAHGAPLLYLLRQPQVIGATIGFAAYNYVFYLVLTWLPSYLS
                     MSLHVDVLHSALDTSIPWLVATFTDLAIGGWLVDALVRRGVRPWLVRQAVLVGGLAFG
                     MGIYGARFAHTPGVALMWISVCMAGLGAMAPVGWSIPTFIAPRASVGRIGGIMNFATQ
                     IAAISAPVITGYFARAHNFGGSFALAAVVLAIGIAGYVLGLGKLKVIPEPETGEFSV"
     gene            complement(1342..1905)
                     /gene="thpR"
                     /locus_tag="VHE33_07025"
     CDS             complement(1342..1905)
                     /gene="thpR"
                     /locus_tag="VHE33_07025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HVT97244.1"
                     /translation="MRLFVGIALPPDVSDALEGIRQQFEPRSGTDLRWSAPEGWHVTL
                     QFLGQVSDERATCVYEQLKTIRASQVPICIDGLGFFDRAGVFWAGVTLTPELLALQQF
                     VTAAMRHCEFTPEDRPYSPHITLARAKGRGSKALQPLQKALEKNKTPLHAEFTAEEFL
                     LYESFPGPKGSRYEIRARFPLKPNSKR"
     gene            2041..2862
                     /locus_tag="VHE33_07030"
     CDS             2041..2862
                     /locus_tag="VHE33_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97245.1"
                     /translation="MSAGRSLLTLCCGVLVPLMTFAQSPVDLPVGSAAPPASDNVRSS
                     SSVAFLELPDAPAPNAPQRTPQNQSQSQTSSSGQKQNEQQQPQSGNAPTLGDLGFTPQ
                     QTLANAEMQKRLNQRTHDLKMHQTLGLITAVPMCATVLVAGGAKSKRDRRNANGTVTG
                     PSTSGVDLHVALGSATVALYGATAYYAIIAPKIPGVEPKGAIRWHRDLVWIHAPGMVL
                     TPILGAMALNQENNGEHVHGIASAHSYVAWTTVLAYGASIVAVSWPIRLKFWEKK"
     gene            2859..>3188
                     /locus_tag="VHE33_07035"
     CDS             2859..>3188
                     /locus_tag="VHE33_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97246.1"
                     /translation="MKFVVGLLLAAAPVLVQAQSQWVLTQSALTWHVTHPMHEVAGTT
                     HAAKGKGNCSAGQCDFLIAVPVNTFDSGDSNRDLHMLEAVRGAQNPMVIVRTHLAESA
                     LNQPTIDA"
BASE COUNT          557 a          888 c         1107 g          636 t
ORIGIN      
        1 tgggccatcg cgccgtctac aattgccaaa gcctcatgag cgaaaacgcc ccaacggcat
       61 ccgcgcacgc gccgcatcgc cgctggcgca ttgcggtgtt gctcggcatc ggggtgctgg
      121 tgaatttctt tgaccgggtg aatgtctcgg tcgcgttcac ttcgctgcac gccgagtggg
      181 gcatctcggc ggtcaccttc ggctatctgg ccagcgccta caactggagc tacggcgcgc
      241 tgcaggtgcc gatcggcgtc atgctcgatc gctggggggt gcggcgcatc gggcgcgtgg
      301 cggcgttcct gtggagcgcg gcttcgatcg catcgggact ggcgaccggc gtgtatagct
      361 tttttgccgc gcggttgctg ctgggcattg gcgaagcgcc gacgtttccc gcgaatgcga
      421 aggcggtggg ctactggttt cccgagcacg agcgcagctt cgccacctca ctcaacgatg
      481 cggcggcgaa attcgcctcc gcgattggcg tgcccatctt gggcgtgttc gtgatctcgc
      541 tgggctggcg gtggagcttc ctcttcacag ggttcatcag cttcctctat ttcctgttgt
      601 tttggcgtgt gtatcgcaac cccagcgagg atccccgcct gagcccggcg gagaggcagt
      661 tgctcgtgga aggaaaggcg cagcctgaga tagcgccgga acatcgcgcg cacggggcgc
      721 cgctgctgta tctgttgcga cagccgcagg tgatcggagc gacgatcgga ttcgccgcct
      781 ataactacgt cttctatctg gtgctcacgt ggctgccgag ttatctgtcg atgagcctgc
      841 atgtggatgt gctgcactcg gctctcgata cgagtattcc gtggctggtg gcgaccttca
      901 ccgatctggc aatcggcggc tggctggtcg atgcgctggt gcggcggggc gtgcggccgt
      961 ggctggtgcg gcaggcggtg ctggtgggcg gactggcatt cggcatgggc atctacggag
     1021 cgcgattcgc gcatacgccg ggtgtcgcgc tgatgtggat cagcgtgtgc atggcgggac
     1081 tgggcgcgat ggctccggtg ggctggtcga ttccgacgtt catcgctccg cgcgccagcg
     1141 tgggacgcat tggcggcatc atgaattttg ctacccagat cgcggcgatc tccgcgccgg
     1201 tgatcacggg atattttgcg cgggcgcata acttcggcgg atcgtttgcg ctggcggcgg
     1261 tggtgctggc gatcgggatc gcagggtatg tgctgggatt ggggaagctg aaggtgatac
     1321 cggagcccga aacgggagag tttagcgttt agagtttggc ttcagcggga agcgggcacg
     1381 gatttcatac ctcgagcctt tgggtccggg gaaggattcg tagaggagaa attcctctgc
     1441 ggtgaattcc gcgtgaagcg gagttttgtt cttctcgaga gccttctgca gaggttgcag
     1501 cgctttcgaa ccgcggccct tcgcgcgcgc cagcgtgatg tgcggactat aagggcggtc
     1561 ttcgggggtg aattcgcaat ggcgcattgc agcggtaacg aattgctgca gagcgagcag
     1621 ctccggtgtg agggtgacgc ccgcccagaa cacgcccgca cgatcgaaga atcccaggcc
     1681 gtcgatgcag atgggaacct gcgatgcgcg aatggttttg agctgctcgt acacgcaggt
     1741 tgcacgctcg tcgctgacct gtcccaggaa ctgaagcgtg acgtgccacc cctcgggggc
     1801 agaccagcgt aagtcagtac cggagcgagg ctcgaattgt tggcgaatgc cttcgagcgc
     1861 atccgatacg tccggcggca gggcgatgcc gacaaaaagc ctcatgcaga cagagtagcg
     1921 aagcagccat ctatccagcg catgaagaat tgttcagatg atagcgatcg atggcaggtc
     1981 gatcctatga tcagacttcg ttcgaccgcg tggcgaggag ggggccgtcg tttgagaggc
     2041 atgagtgcgg gaagatcgct gctgactctt tgctgtgggg tgctggttcc gctcatgacg
     2101 ttcgcgcaat cgccggtgga tctgcccgtg ggttcggccg ctccaccagc ttcggacaac
     2161 gtccggtcat cgtcttcagt cgcgtttctc gaactgcccg acgctccggc gccgaacgcg
     2221 ccgcagcgta caccacaaaa tcagagtcag tcgcagacgt cgtcgtccgg gcagaagcaa
     2281 aacgaacagc aacagccgca gtccggcaac gcgccgacgc tgggcgatct gggattcacg
     2341 ccgcagcaga cgctggcgaa tgccgagatg cagaagaggc taaaccagcg gacgcacgat
     2401 ctgaagatgc atcagacgct ggggctgatt acagcggtgc cgatgtgcgc gacggtgctg
     2461 gtggcgggcg gggcgaagtc gaagcgcgat cgcaggaatg cgaacggcac ggtcacgggc
     2521 ccgagcactt ccggcgtcga tctgcacgtt gctttggggt cggcgacggt cgcgctctat
     2581 ggagcgacgg cctactacgc gatcattgcg ccgaagattc cgggcgtgga gccaaaggga
     2641 gcgattcggt ggcatcgtga tttggtttgg atccacgcgc cgggcatggt gctgacgccg
     2701 atcctcggcg cgatggcact gaaccaggaa aacaatggcg agcacgtgca cggcatcgcg
     2761 tcggcgcatt catatgttgc gtggacgacg gtacttgcct acggggcgtc gatcgtcgcg
     2821 gtctcgtggc cgatccgtct caaattctgg gagaagaaat gaagtttgtg gttgggcttc
     2881 tgctggcggc cgcgcccgtc ctggtgcagg cgcagagtca gtgggtgctg acgcagtccg
     2941 cgctgacgtg gcatgtaacg catccaatgc acgaagtagc aggaaccact cacgcggcga
     3001 aaggcaaagg caactgcagc gcggggcagt gcgattttct gattgctgtg ccggtgaaca
     3061 cgtttgactc gggcgacagc aatcgcgatc tgcatatgct ggaggcggtg cgcggcgctc
     3121 agaacccgat ggtgatcgtg agaacgcacc tggcggaatc ggcgctcaac cagccgacga
     3181 tcgacgcc
//
LOCUS       DASZYN010000272         3147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_393008, whole genome shotgun sequence.
ACCESSION   DASZYN010000272 DASZYN010000000
VERSION     DASZYN010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393008"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VHE33_07040"
     CDS             complement(<1..981)
                     /locus_tag="VHE33_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HVT97247.1"
                     /translation="MTDTPAQLAAEHIVIRLRAAGYEAYFAGGCVRDILLGVEPKDYD
                     VATSAHPDVVLKMFPRTFAVGAQFGVVLVCQDLDDPANAGHTREIVTEVATFRSDVAY
                     LDGRHPTEVRFSATPQEDVIRRDFTINGMMFDPVLNRQTCNLTSAVLDYVGGRSDLRD
                     GIIRAIGEPHRRFEEDKLRMLRAVRFAARFEYAIEPKTERAIRQLAAGIAQVSRERVR
                     DELTKMLTEGHARRAFELLDSTGLLREVLPEVTKLRGVEQPPEYHPEGDVWVHTLLLL
                     EKLPAGVSPTLAWGALLHDIGKPATFSHNPPDRIRFNGHVEVGVKIAHAIL"
     gene            complement(1035..2588)
                     /locus_tag="VHE33_07045"
     CDS             complement(1035..2588)
                     /locus_tag="VHE33_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97248.1"
                     /translation="MGATVLQLDKPAASRADQAARSVLIGLLCSLAAFICMHGAAGSV
                     ADPDAGWHLRTAEWILQHRALPHVDPFSRTTGGSPWQAYSWLFELILLKAYGWMHLRG
                     LLVFTAAMMTLISAALYRMMSRLQSDFMSCAVLNLIVIFCLSGLMTPRPWLFTILFFV
                     IELDILMHARREGTTRELWLLLPLFALWANIHIQFVDGLLVLGLAAIEPLVERWWKSN
                     VRGVGARPLWLALGGSLLAVCVNPYGFAIYKIAWSLGSQSGVLNTVSEMGAMKFRSPQ
                     DFLLLFLALAAAGVLFRYRRLNPFETMLLAMAAVLSFRSSRDLWIIVIAASAILAAGL
                     PVRASRETHEKRPLWAPAFAVLTGAAAFAASVFFVQTDNAALQKRLAERMPVQAVQFV
                     KDHHYNGALFNTYDWGGYLIWNLREPVSIDGRAALYGDQAISRSAATWGGGPQWAADP
                     DLASAGVVIGPAGAALTQLLRNDARFTLAYEDKVAAVFVAREKSKAAGQASPNGSPVA
                     HEQQNPLPR"
     gene            2861..>3147
                     /locus_tag="VHE33_07050"
     CDS             2861..>3147
                     /locus_tag="VHE33_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97249.1"
                     /translation="MPSPLPGLILGIETSCDETAAAVVRRGREILSNTVASQIAVHLP
                     YGGVVPELASREHLRNILPVVRESLKTAGVGYGDIDAIAVTEGPGLAGALLV"
BASE COUNT          651 a          937 c         1023 g          536 t
ORIGIN      
        1 caggatggca tgcgcgatct tcacgccaac ctcgacatgc ccgttgaagc ggatgcgatc
       61 gggtggattg tgggaaaacg ttgccggctt gccgatgtcg tgcagcagcg caccccaggc
      121 gagcgttggc gagacgccgg cggggagctt ttcgagcaac agcaaggtgt ggacccagac
      181 gtctccctcg gggtgatact ccggtggctg ctcgacgccg cgcagttttg tgacctcggg
      241 caggacttcg cgcagcaggc ccgtagaatc gagcagctcg aaggcgcgac gggcatgacc
      301 ctcggtgagc atcttcgtca attcgtcgcg aacgcgctcg cggctgacct gcgcgatccc
      361 cgccgcgagt tgtcggatag cgcgctcagt tttcggttcg attgcgtact caaaacgcgc
      421 ggcaaaccgc acagcgcgga gcatacggag tttgtcctct tcgaaacggc gatgcggctc
      481 gccgatggcg cggatgatgc cgtcgcgaag gtcgctgcgt ccgcccacat aatcgagaac
      541 agcagacgtg agattgcagg tctgtcggtt tagaacggga tcgaacatca tgccgttgat
      601 cgtgaagtcg cgacggatca cgtcttcctg aggcgtggcg gagaagcgga cctcggtggg
      661 gtggcggcca tcgaggtaag cgacatcgga gcgaaatgta gccacttcgg tgacaatttc
      721 tcgggtgtga cccgcattgg cgggatcgtc gaggtcctga cagacgagga cgacgccgaa
      781 ctgcgcgccg acggcgaagg tgcggggaaa cattttgagc acgacgtcgg gatgcgcgct
      841 ggtggcaacg tcgtagtcct ttggttcgac gccgagaagg atgtcgcgca cgcatccgcc
      901 cgcgaaatac gcctcatagc ccgccgcacg aagtcggatg acaatgtgtt cggcggcgag
      961 ttgggccgga gtgtcggtca taacggcagg atgccgcagg gcgatgaggg gtcgccggca
     1021 tctgccctct gagtctacct ggggagcggg ttttgttgtt cgtgcgctac gggcgacccg
     1081 ttcggtgatg cctggcccgc tgctttactt ttttcgcgtg cgacaaacac cgccgccacc
     1141 ttgtcttcat aggccagagt gaagcgggca tcgttgcgca gcagctgcgt gagcgccgcg
     1201 ccggcggggc cgatcaccac tccggccgag gcgaggtcgg gatccgcggc ccactgggga
     1261 ccgccgcccc acgtcgcggc cgatcggctg atggcctgat cgccgtaaag agcggcgcgg
     1321 ccgtcgatac tgaccggctc gcggagattc caaataaggt agccgcccca gtcgtaggtg
     1381 ttaaagaggg cgccgttgta atgatgatcc ttcacaaact ggactgcctg gaccggcatc
     1441 cgctctgcga gccgtttctg cagagctgca ttatccgtct gcacgaagaa cacactggcg
     1501 gcgaaagcgg cggctcccgt cagcacggcg aatgcgggtg cccacaaagg ccgcttctca
     1561 tgcgtttccc ggctggcgcg gacgggaaga ccagcggcca ggattgcgct tgcggcgatg
     1621 acgatgatcc agagatcgcg gctcgaacga aaggacaaca cggcggccat cgcgagcagc
     1681 atcgtctcga agggattgag gcggcggtag cggaacagca cgcccgccgc agccagcgcg
     1741 aggaacaaca acaggaaatc ctggggactg cgaaacttca ttgcccccat ctcgctgacg
     1801 gtattcagga caccggactg cgaaccgaga ctccacgcga tcttgtagat ggcgaagcca
     1861 taaggattga cgcagacggc cagaaggctt ccgcccagcg cgagccacag gggccgggcg
     1921 cccacaccgc gcacattcga cttccaccat cgctcgacga gaggctcgat cgccgccagt
     1981 ccgagcacca gaagcccatc cacaaactga atgtgaatgt tcgcccacag cgcaaacaga
     2041 ggaagcaaca gccacagctc gcgggttgtg ccttcgcggc gcgcgtgcat cagaatgtcg
     2101 agttcaatga cgaagaacag gatggtgaac aaccacgggc ggggcgtcat caggccggaa
     2161 aggcagaaga tcacgatcag gttcagcacc gcgcaggaca tgaagtcact ctgcagccgg
     2221 ctcatcatgc ggtacaaagc cgccgaaatg agcgtcatca tggcggcggt aaaaaccagc
     2281 aggccgcgca gatgcatcca gccgtaagcc ttcagcagaa tcagctcgaa gagccagctg
     2341 tacgcctgcc acgggctccc gccggtagtg cgagagaagg gatcgacatg gggaagagcg
     2401 cgatgctgaa gaatccactc tgcggttcga agatgccagc ctgcgtccgg atcggcaaca
     2461 gatccggccg ccccatgcat acaaatgaag gccgcaagcg aacacagcaa tccgatgaga
     2521 accgacctgg ccgcctgatc ggcgcgggat gctgcgggct tatccaactg taaaacggtc
     2581 gctcccatgc ttgtgtgccg ccgtgccgaa acgatcgcct tccggcaaca acgcccggtt
     2641 cgtttcctct cctgccgcgc ggcttcacca cgcaacgacg ccttcatcat gatccggatg
     2701 atggatgtgc ctgtcgatac ccagccggga tactggggta acacagcggt ccgcagctac
     2761 cacaaaggtg gcacgagggc tgcccggcga tcagcaccat gggaagtgtg atggcggtgc
     2821 tttcgccgga cgcatcgtcg cgctgattaa tataaaaaga atgccctcac ccctcccagg
     2881 cctgatcctc ggaatcgaaa cgtcgtgcga tgagaccgcc gctgccgtgg tccgccgcgg
     2941 cagggagatc ctgtcgaaca cggtagcgtc acagatcgca gtgcatttgc cgtacggggg
     3001 cgtggtgccg gagctggctt cgcgcgagca tttacgaaac attctgccgg tggtgcggga
     3061 atcgctgaaa acggccggag ttggatatgg ggatattgat gccatcgcgg ttacagaagg
     3121 acccgggctt gccggtgcat tgctggt
//
LOCUS       DASZYN010000273         7764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1232255, whole genome shotgun sequence.
ACCESSION   DASZYN010000273 DASZYN010000000
VERSION     DASZYN010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7764
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232255"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VHE33_07055"
     CDS             complement(<1..539)
                     /locus_tag="VHE33_07055"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97250.1"
                     /translation="MMSTGSSEVLTGPPVAARRHTEKTVHGHTLVDDYAWLQEKESPE
                     VLAYLEAENAWCQQVMAGSEDLQKQLYEEMRSHIKETDISVPFRDRDYWYYQRTEEGR
                     QYEIYCRKRGTPEGPTGEEELLLDVNELAEHEAFMALGTMVVSDDGNLLAYSCDNKGF
                     RQYKLYVKDLRTGEVFKEHA"
     gene            complement(680..1279)
                     /locus_tag="VHE33_07060"
     CDS             complement(680..1279)
                     /locus_tag="VHE33_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HVT97251.1"
                     /translation="MRAFVLAGTGLLVALGATAQQQDILHPKDTSALHPPPGSKVAIV
                     EFADLECPSCGMDNPIIQQAVDKYHVAWERHDFPLPQHNWSFQAAVNARWFDTKSKDL
                     GNQYRNAVFANQGSIETKSDLQNFTQKFAQQHGTALPFVMDPQGKLTAEVKADAALGD
                     RMGIHETPTAWVVTAHGYKQITDFRNLYTMLDEAVANNH"
     gene            complement(1556..2287)
                     /gene="ccsA"
                     /locus_tag="VHE33_07065"
     CDS             complement(1556..2287)
                     /gene="ccsA"
                     /locus_tag="VHE33_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HVT97252.1"
                     /translation="MKLWRWLWLVVTLGVLVEGFHVALFVVPRDAAQGDVARVIYYHV
                     PSWAGMGLFFAINLGCSLVYLAVRNRNQALAIKADSLAVASAEMGVVFCLIGLVTGSL
                     WGRAVWGIWWTWDARLTSTLVLWLIYVAYLLLRRFAAGPQMRTLAAVMAIFGYLDVPL
                     VYMSTRWWRTQHPAPVFGGAPGSGLDPSMMPAIWWNLAGWLMWGIFVASLRYASEYRA
                     QKAEQEEALKALDYVPGQAQMAGGR"
     gene            complement(2337..2939)
                     /locus_tag="VHE33_07070"
     CDS             complement(2337..2939)
                     /locus_tag="VHE33_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HVT97253.1"
                     /translation="MNKIVHRVLVLGLAMGVVAIPCGRAQEKPEKPPPLPGQGGSADT
                     APSQKTEKAPSQKPEKAPPLPGQKGQDAKSAEQEQSGVTVAIVNRIPPEDKHNLNGYW
                     AGVENKIGQRWQHAAAAQKLTDAVKITGWIHTDGRVTGLMVEHGSGNASVDRAARATI
                     SESAPYDPFPYGIAVDEVKVRFTFGAAAGAPDGPPPAVVH"
     gene            3057..3257
                     /locus_tag="VHE33_07075"
     CDS             3057..3257
                     /locus_tag="VHE33_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97254.1"
                     /translation="MAWTTLILGVLLMAIGIGGQIFGTRKSATLAPHDRPARRWIITV
                     GSLVIGLWIVAISAAHYLHHHH"
     gene            complement(3311..3985)
                     /locus_tag="VHE33_07080"
     CDS             complement(3311..3985)
                     /locus_tag="VHE33_07080"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HVT97255.1"
                     /translation="MKAAYFLTHLRKDLQLEWRSKDAINSMLFFALLVVVLFSLAFDP
                     TRAVSRQIAGGILCVAAMFAAVSALNQAWARELRNQVLDALRMTPAPGASLFLGKTLA
                     NFFFVIVVELVLAPVFVMFYNLHALGNGWLLLLVLPLGTWALIVNGVFFAALSIRTRN
                     RELLLPLILFPIFIPALLGMVMSATSILTGEGNAVMWIKLLGAYDVIFTTVCMLLFEV
                     VLHADG"
     gene            complement(3982..4515)
                     /gene="ybaK"
                     /locus_tag="VHE33_07085"
     CDS             complement(3982..4515)
                     /gene="ybaK"
                     /locus_tag="VHE33_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HVT97256.1"
                     /translation="MSGKTNAARYLDSLGIQYEMREYEVDPEEFSAIVVAEKVGLPPE
                     QVFKTLLCVTSEREHVFAVVPGDAELDFKKLAAAAGTRKTEMVSLKEVQPLTGYVRGG
                     VTVFGAKKDFPVYVDETLELFEVVSVSAGTRGVQVVLSPADYLRASKATVADLTKQGS
                     EHRAQGAGTAERWEKSR"
     gene            complement(4512..5225)
                     /gene="ccmA"
                     /locus_tag="VHE33_07090"
     CDS             complement(4512..5225)
                     /gene="ccmA"
                     /locus_tag="VHE33_07090"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HVT97257.1"
                     /translation="MNGTIAEFEEVSKLYGTFAALRKVSVQFERGRCYLVLGPNGAGK
                     STLLRTLAGLLRPSYGKVKVFGADGAAEDPMEARARIGYMSHATMLYDEFSGVENLRY
                     FASLYRERECVTPEDALRSVGLDPALQRPVGQYSQGMRQRASLARVLISRPELLLLDE
                     PFSNMDIASAHQMLRLLERFRTEQRTILLTTHQRELAEPLADFVLTLEAGAVASLEDG
                     PYATRGAAGLEAATAGVRR"
     gene            complement(5333..5797)
                     /locus_tag="VHE33_07095"
     CDS             complement(5333..5797)
                     /locus_tag="VHE33_07095"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HVT97258.1"
                     /translation="MKNGNKQTVRIALAVVVIVGAISYLAVTGVEANKSYYVTIQELQ
                     SMGNKAYTRNLRVAGSVVPGSIVRDGAHAQFTLVEVDRKLKVSYTGDEPPPDTFKDNS
                     QALAIGTFGRDGVFHATQLQAKCASKYAPAQPGQAKPGATQTAAVAAATSGQ"
     gene            complement(5901..6404)
                     /locus_tag="VHE33_07100"
     CDS             complement(5901..6404)
                     /locus_tag="VHE33_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97259.1"
                     /translation="MSLGMVLWVIAAMAQERGSSAAPPPPTMQMTIVGKSLNASDGKI
                     YVNENCEVFPGLQLPMGDHKPKGEWMPEVCHLENVKEFDRHDEKKAGSDGKGEDVEIR
                     ERDYVLQNISVKPAVFEVLEMIPEGWSVESDPKPAEMDGPVGVFEVKAGPWGEARLHV
                     RLRRVRK"
     gene            complement(6418..>7764)
                     /locus_tag="VHE33_07105"
     CDS             complement(6418..>7764)
                     /locus_tag="VHE33_07105"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HVT97260.1"
                     /translation="YDKILLGLLDSHTLVGDPEALAILEQTTDVALPHLPGHAVEHGT
                     RWRMDHDDESWTWDESYTMPENLFLAYQRGAGRRYFDLGLQYLDDKAWFDPLSRNENV
                     LAGRHAYSYVNSLNSAMMAYLAAGSEKHLHAGRNGFAMVEAQSFATGGWGPDEQLRAP
                     GSGDLLKSLTETHHSFETPCGSYAHFKMTRYLLRVTRDAHYGDSMERVMYNTVLGAKP
                     LEENGRTFYYSDYNFDGKRFYHRDRWACCSGTLPQVAADYRICAYFRGAQSVYVNLYV
                     PSTLRWKDRGTAFSLTQEGQYPFEENVTVTVTCSRPAEQTLYFRIPAWARGAAVAVNG
                     VRQRQGVEPGRFAAVRREWKNGDRVELELPLAMRLDAIDEQHPDTVALLRGPVVLMGV
                     KPEPGNAMPAMQRAQLLGARRRSEREWRVETDRGSVRLVPFVAVGDLPYTTYVKVS"
BASE COUNT         1509 a         2915 c         1827 g         1513 t
ORIGIN      
        1 gcgtgctctt taaagacttc ccccgtccgc agatccttca cgtagagctt gtattggcgg
       61 aatcctttgt tatcgcagga atacgccagc agattcccgt catcggaaac gaccatcgtc
      121 cccagcgcca tgaacgcttc gtgctctgcc agttcattga catccagcag aagctcctct
      181 tcccccgtcg gaccctcggg cgtaccgcgc tttcggcaat aaatctcgta ttgccttccc
      241 tcctcggttc gctggtagta ccagtaatcg cggtcgcgaa acggaaccga aatatcggtc
      301 tccttgatat gactccgcat ctcttcatag agctgtttct gcaggtcctc gctccccgcc
      361 atcacctgct gacaccacgc gttttccgcc tccagatacg ccagcacctc agggctctcc
      421 ttttcctgta gccaggcgta gtcgtccacc agcgtgtgac cgtgcaccgt cttttccgta
      481 tgccggcgcg ccgcgacagg cggcccggtc aggacctcac tgcttccagt gctcatcatc
      541 cctctcgtgt caggaaatct cagaagcgca atctatagat gcagaatggc ccgccaggct
      601 ttgtgcctgg agcacgcatt tttcagctgt gccggacaac ccgggggtcg gcaccgccat
      661 actggcgagt ggctgcagcc tagtggttgt tcgccaccgc ctcgtccagc atggtgtaca
      721 gatttcggaa gtcggtgatc tgcttgtagc cgtgggccgt caccacccac gccgtcggcg
      781 tctcgtggat gcccatccgg tcccccagcg ccgcatctgc cttcacttcg gccgtcagct
      841 tgccctgcgg atccatgaca aacggcagcg ccgtcccatg ctgctgcgca aatttctgcg
      901 tgaaattctg cagatcgctc ttcgtctcga tgctgccctg gttcgcgaac accgcgttgc
      961 gatactgatt ccccagatcc ttcgatttcg tatcgaacca gcgcgcattc accgcggcct
     1021 ggaagctcca gttgtgctgc ggcagcggaa agtcatgccg ctcccacgcc acgtggtact
     1081 tatcgacggc ctgctggatg atcgggttgt ccatcccgca gcttggacac tccaggtccg
     1141 caaactccac gatggccacc ttcgaacccg gcggcgggtg caaagccgag gtgtctttcg
     1201 ggtgcagaat atcctgctgc tgggcggtcg cccccagcgc aacaagcaaa ccggttcctg
     1261 ccagaacaaa agcgcgcatt ccaaacataa ttcgagtcta caaaatcccc cgcccgcccg
     1321 ccggggcgag ccagaacaga gctaatttga gatggacgaa acagaaccgg aatcatgagc
     1381 aggaaaatga ccccgaagcc ccgcgatcaa gggccgggct ggccattggt tcctggctac
     1441 tggctattcc ctctcttccg gctcttcaac gccacataca ccagatacag cagatgaatc
     1501 ccgatcgtcg ccgcgtacgc cagcaccaga tgccgatgtc ccatgtcgcc catcgctatc
     1561 gccctcccgc catctgtgcc tgccccggca cgtagtccag cgccttcagc gcctcttcct
     1621 gctccgcctt ctgtgcccga tactccgacg cataccggag gcttgccaca aaaatgcccc
     1681 acatgagcca cccggccaga ttccaccaga tcgccggcat catgctcgga tctaaacccg
     1741 aacccggtgc gccaccaaac accggcgccg gatgctgcgt ccgccaccac cgcgtcgaca
     1801 tatacaccag cggcacatcg agataaccga agatcgccat caccgccgcc agcgtccgca
     1861 tttgtggccc ggccgcaaac cgccgcaaca gtaaatacgc gacgtagatc agccacagca
     1921 ccagggtcga cgtcagccgc gcatcccacg tccaccagat gccccacacc gcgcgtcccc
     1981 acagcgaccc ggtaaccagg ccgatcaggc agaacactac acccatctcc gccgacgcca
     2041 ccgccagtga atccgccttg attgccaggg cctgattccg gttccgcacc gccagataca
     2101 cgagcgaaca gcccaggttg atcgcaaaga acaaccccat ccccgcccac gatggcacgt
     2161 ggtaataaat gactcgcgcc acatcgccct gggccgcatc ccggggcaca acgaacagcg
     2221 cgacgtggaa accctccacc agcactccca gtgtcactac cagccataac caacgccaca
     2281 gtttcataac cgcctctttc ctgcgcgcat cctgccaatc tgccgatcga actgcatcaa
     2341 tgcaccaccg ccggaggtgg accatccggc gccccagccg ccgcgccaaa tgtgaaccgc
     2401 accttcacct catccactgc aatcccataa ggaaacggat cgtaaggggc cgattcgctg
     2461 atcgtggcgc gcgcagctcg atccacactc gcattgcccg acccatgttc caccatgagc
     2521 cccgttaccc ggccgtccgt gtgaatccag ccggtgatct tcaccgcatc ggtcaacttt
     2581 tgggcagctg ccgcgtgctg ccaccgctgc ccgatcttgt tctccactcc cgcccaatag
     2641 ccattcaaat tgtgcttatc ttccggcgga atccgattca cgatcgcaac cgtgaccccg
     2701 ctctgttcct gctccgccga tttcgcatcc tggccctttt gccctggcag cggcggcgcc
     2761 ttctccggct tctgcgatgg agccttctcc gttttttgcg atggcgccgt atccgcgctg
     2821 cctccttgac ccggcagcgg cggtggtttt tccggctttt cctgcgccct tccgcacgga
     2881 atggctacga cccccatcgc caagcccagc accagcaccc tgtgaacgat cttgttcatt
     2941 cctttgcctc cgcatcgacc cggtgcacca cgccctgcat acccaaaacc ttactcctcg
     3001 gacctcgctc ctgcctcttc ccgcatccac ctccgatagg gtacaaaggt tagaccatgg
     3061 cttggaccac cctcatcctc ggcgttctcc tcatggccat cggtatcggc ggccagatct
     3121 tcggcacccg taaatctgcc actctcgctc cccacgaccg ccccgcgcgc cgctggatca
     3181 tcaccgtcgg ctccctcgtc atcggcctct ggattgtcgc gatctccgcc gctcattacc
     3241 tccatcatca ccactaggtt gatcgaccat ccttcgtgaa aagggggcca ggcccgtccg
     3301 ctctcggcgg ctagccgtcc gcgtgtaaaa ccacctcaaa cagcaacatg caaaccgtcg
     3361 tgaagatcac gtcataggcc cccagcagtt tgatccacat cacggcattg ccttcacccg
     3421 tcagaatcga cgtcgccgac atcaccatcc ccagcaatgc gggaatgaaa ataggaaaaa
     3481 ggatcagggg cagcagcaat tcccgattcc gcgtccgaat cgacagcgcc gcaaagaaca
     3541 cgccgttcac aatcagggcc cacgtcccga gcggaagcac caacagcaac agccagccat
     3601 tgcccagcgc gtgcaggttg tagaacatga cgaagaccgg tgccagcacc agttccacca
     3661 caatcacaaa gaaaaagttc gccagcgtct tgcccaggaa cagcgacgcg cccggggcgg
     3721 gcgtcatccg cagcgcatcc agcacctgat tccgcagctc ccgcgcccac gcctgattca
     3781 gagcactgac cgcggcaaac atcgccgcca cgcatagaat tccacccgca atctgccggc
     3841 tcaccgcgcg cgtgggatcg aacgccaaac tgaacaacac caccaccagc agggcaaaga
     3901 acagcatgga gttgatggcg tctttcgatc gccactccag ctgcagatcc ttcctgagat
     3961 gggtaaggaa atacgcggcc ttcatcgcga tttctcccag cgctcggccg tgcctgcgcc
     4021 ctgagccctg tgctctgagc cctgtttcgt caaatccgcc acggtcgcct tcgacgcccg
     4081 cagataatcc gccgggctca gcaccacctg cacaccccgc gtccctgccg aaacagaaac
     4141 cacctcgaac agctccaacg tctcgtccac gtacaccggg aaatccttct tcgccccaaa
     4201 caccgtcacc ccaccccgca cataacccgt cagcggctga acctccttga gcgaaaccat
     4261 ttccgtcttc cgcgttcccg cagccgccgc cagtttcttg aaatccagtt ccgcatctcc
     4321 tggcaccacg gcaaatacat gctcccgttc cgatgtcacg cacaacaatg tcttgaacac
     4381 ctgctccggc ggcagtccaa ccttctccgc caccacaatc gcagaaaact cctccggatc
     4441 aacctcgtac tcgcgcatct cgtactggat cccaagcgaa tccagatacc gcgccgcgtt
     4501 ggtctttccg ctcatcgccg aacccctgcc gtcgccgctt ccagccccgc ggcaccgcgc
     4561 gtcgcatagg gtccatcttc caaactcgcc actgcccccg cttccagcgt cagcacaaaa
     4621 tccgccagcg gctcggccag ctcccgctga tgcgtcgtca gcagaattgt tctctgctcg
     4681 gtcctgaacc gttccagcag ccgcagcatc tggtgcgcgc tcgcaatatc catgttcgaa
     4741 aacggctcat ccagcagcag cagctccggt cgcgaaatca gcacccgcgc cagcgacgcc
     4801 cgttgccgca tcccctgcga gtactgccca accggtcttt gcaaagcagg gtccagcccc
     4861 acgctgcgca acgcatcctc cggcgttacg cactcccgct cccggtacag gctggcaaaa
     4921 taccgcagat tctctacgcc ggaaaattcg tcatacagca tcgtggcgtg gctcatgtat
     4981 ccgatgcgcg cacgcgcctc catcggatct tctgccgccc catccgcgcc gaacaccttt
     5041 accttcccgt agctcggccg caacagcccc gccaaagtcc gcagcagcgt cgacttcccc
     5101 gctccattcg gccccagcac cagatagcac cgtccgcgct cgaactgaac cgacactttg
     5161 cgcagcgccg caaacgtgcc gtacagcttc gatacttcct cgaactccgc aatcgtgccg
     5221 ttcatcgtcg cttcctgcgc agtctactcc gcccgcccgt ttccacacac ctcaaccgag
     5281 ccgattcctg ccgctttttc tgaacgctgt acgctgaccg ctgaacgctg tcttattgcc
     5341 ccgatgtcgc tgcagcgacc gcagccgttt gcgttgcgcc cggctttgcc tgacccggct
     5401 gcgcgggcgc atacttcgac gcgcactttg cctgcagctg cgtcgcgtgg aacaccccat
     5461 cccgtccaaa agtcccgatg gccagcgcct ggctgttgtc cttgaaggtg tccggcggcg
     5521 gttcgtcgcc cgtgtaagaa accttcagtt tccggtccac ttccaccagg gtgaactgcg
     5581 catgcgctcc gtcgcgcaca atgcttcctg ggaccacgct tccggccacc cgcagattgc
     5641 gcgtataggc tttgtttccc atcgactgca gttcctggat cgtcacgtag tagctcttgt
     5701 tcgcctcgac tccggttacc gccagatacg agatcgcgcc tacgatcacc acgactgcca
     5761 gggcgatgcg caccgtctgc ttgttgccgt tcttcatgat gactctattg taagcagccc
     5821 cgcccaaacc cggcatgtga catagatcac tggccaaaaa agctctgggc tcagcgcctt
     5881 ccgcccctcg ccacccggct tcacttcctg acgcgccgta atcgaacgtg cagccgcgcc
     5941 tcgccccagg ggcccgcctt tacctcgaag acccccaccg gcccatccat ttccgccggt
     6001 tttggatccg attcgacact ccacccctcc ggaatcattt ccagcacttc gaataccgcc
     6061 ggcttcacgc tgatgttctg cagcacataa tcccgttcgc gaatctccac atcctcgccc
     6121 ttgccgtcac tccccgcttt cttctcgtcg tgccggtcaa attcctttac gttctccaga
     6181 tggcaaacct ccggcatcca ctcgcccttc ggcttgtgat cccccatcgg caactgcagg
     6241 ccaggaaaga cttcgcaatt ctcattgacg taaatctttc cgtcgctcgc attcagtgat
     6301 ttacccacaa ttgtcatctg catcgtcggc ggaggcggcg ccgcggatga gcccctctcc
     6361 tgcgccatcg ccgcaatcac ccacaacacc atccccaggc tcacagcttc acgctcctca
     6421 gctcaccttg acataagtcg tgtacggcaa atcccccacg gcaacgaagg gaaccagcct
     6481 caccgaaccg cgatccgtct ccacccgcca ctcgcgctcg ctcctccgcc tcgcccccag
     6541 caactgcgct cgctgcatcg ccggcattgc gttgcctggt tccggtttga ctcccatcag
     6601 cacaaccggc ccccgcagca aagccacggt atccggatgc tgctcgtcga tcgcatccag
     6661 gcgcatcgcg agcggcaact ccagctccac gcgatcgcca ttcttccact cccgccgcac
     6721 cgccgcaaat cgccctggct cgacgccttg cctctgccgc acaccattca ccgcaaccgc
     6781 agcgcctcgc gcccaggccg gaatcctgaa gtacagcgtc tgttccgccg gccgtgaaca
     6841 cgtcaccgtc accgttacat tttcttcgaa cggatactgt ccctcctgcg tcagtgaaaa
     6901 cgccgttccc cgatccttcc accgcagcgt cgacggaaca tacaaattca catacaccga
     6961 ctgcgccccg cgaaaatacg cgcaaatccg gtagtcagcc gccacctgcg gcagtgttcc
     7021 cgagcagcac gcccagcgat cgcgatggta aaacctcttt ccgtcgaagt tgtagtccga
     7081 gtagtaaaac gtccgcccgt tttcctccaa cggcttcgcc cccagcacgg tgttgtacat
     7141 cacccgttcc atgctgtcgc catagtgcgc atcgcgcgtc acgcgcaata aataccgcgt
     7201 catcttgaag tgcgcatacg acccgcacgg cgtctcaaag ctgtggtgcg tctccgtcag
     7261 gctcttcagc agatcgccgc tccctggcgc ccgcagctgt tcatcaggac cccacccgcc
     7321 tgtcgcaaaa ctctgcgcct ccaccatcgc aaatccattc cgccccgcat gcaaatgctt
     7381 ctcgctgccc gccgccaggt acgccatcat cgcggagttg agcgagttca catagctgta
     7441 cgcatgccgc ccagccagca cattttcatt gcgcgacagc ggatcgaacc acgccttgtc
     7501 atcgaggtac tgcagcccca gatcgaagta ccttcgcccc gcaccccgct gatacgccag
     7561 aaacagattc tccggcatcg tgtacgactc gtcccacgtc cacgactcgt cgtcgtgatc
     7621 catcctccat cgcgtgccat gttctacggc gtgtccaggc aaatgcggca gcgcgacatc
     7681 cgtcgtctgc tccagaatgg ccagcgcctc cggatccccc accagcgtgt gcgagtccag
     7741 caaccccagc agaatcttgt cgta
//
LOCUS       DASZYN010000274         4112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_75600, whole genome shotgun sequence.
ACCESSION   DASZYN010000274 DASZYN010000000
VERSION     DASZYN010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75600"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /gene="rplA"
                     /locus_tag="VHE33_07110"
     CDS             <1..489
                     /gene="rplA"
                     /locus_tag="VHE33_07110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HVT97261.1"
                     /translation="VAVITTGEKQREAEAAGADIVGGDELVEKIQKESWTDFDALIAT
                     PDMMKSVGRLGKVLGPRGLMPNPKTGTVTNDVTAAIKEIKAGKVEFRTDKTALVHVPV
                     GKISFPPQKLVENAITVITSVVKAKPSAAKGKYIKGITISSTMGPGIALDSAVADSAA
                     KA"
     gene            607..1155
                     /gene="rplJ"
                     /locus_tag="VHE33_07115"
     CDS             607..1155
                     /gene="rplJ"
                     /locus_tag="VHE33_07115"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HVT97262.1"
                     /translation="MALSRQKKAEKVGELAKELETSTSAIIGTFSKLTVAQDFELRKT
                     VRNAGGRYRVLKNKLAGRAAKGTKIEAALQGLKGVSSVAYTSGDPVALAKALSTWVSD
                     NAEFTFKLGIVDGKVINVEEVKALATMPGKEEIFSKLLYLINAPAQRLVTVMNAAGRD
                     LAVVINQGVEKNKFTGGEAAAS"
     gene            1355..1735
                     /gene="rplL"
                     /locus_tag="VHE33_07120"
     CDS             1355..1735
                     /gene="rplL"
                     /locus_tag="VHE33_07120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HVT97263.1"
                     /translation="MADLQQLEDQIVNLSLLDAAALVKKLEERLGVSAAAAAPVVVAG
                     GGAAGAAAPAAVEQTEFTVTLKDAGANKINTIKAVREVTSLGLKEAKDLVDGAPKTLK
                     ENISKEDAEAIKKKFEGVATIEVK"
     gene            2547..>4112
                     /locus_tag="VHE33_07125"
     CDS             2547..>4112
                     /locus_tag="VHE33_07125"
                     /inference="COORDINATES: protein motif:HMM:NF016450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97264.1"
                     /translation="MSNENRAVRSRLDFSKIPTAIRIPNLIEVQRRSYERFLQMDKLP
                     SERDDSGLQSVFTSVFPITDFRNISQLDFVDFAIGNWECKCGHLKGLHHLRTTCVHCG
                     AMVITDPFHPGDVLCQKCGTYNKNTPDFCNKCGDPVGLQLKYDQQECEERGMTFSAPL
                     KVTVRLTIYDKDPETGAKSIRDIKEQEVFFGDIPLMTQNGTFIVNGTERVIVSQLHRS
                     PGVFFETANNRTYFLGKIIPYRGSWVEFEYDQKNTLYVRIDRKRKFLGTIFLRALGLK
                     SDEEILRTFYTVDKINIKDGKLLWTLDADKPTHLLGAKPAHAIVVKGEEIAHSGRKVT
                     PSILKALRAAKVAHVEVEQSELDGAMTAADVVDTSTGEVFLEANQELTSDKLHKIMEA
                     GISTIDVFFPERDDMGNVITNTLRRDSVRKPEEALIEIYRKLRPGDPPTLDTATALFE
                     GMFFDPRKYDFSRVGRLKFNIKLYENQDATSLDHRTLTPDDFYSTIRYLLKLRRNIGM
                     VDDIDHLGNRRVRA"
BASE COUNT          892 a         1363 c         1093 g          764 t
ORIGIN      
        1 gtcgccgtca ttaccaccgg cgaaaagcag cgcgaagccg aagctgccgg cgccgacatc
       61 gtcggcggcg acgagctcgt cgaaaagatc caaaaggaat cctggaccga cttcgacgct
      121 ctgatcgcca cgcccgacat gatgaagtcc gtcggccgtc tcggcaaagt cctcggcccg
      181 cgcggcctca tgccgaaccc caaaaccggc accgtgacca atgacgtcac tgccgccatc
      241 aaggaaatca aagccggcaa ggtcgagttc cgcaccgata agaccgcgct cgtccacgtc
      301 ccggtcggga agatctcctt ccccccgcaa aagctggttg aaaatgccat tacggtcatt
      361 accagcgtcg tgaaggcgaa gccttctgcg gccaagggaa agtacattaa gggcatcacc
      421 atcagctcca ccatgggccc tggcatcgcg ctcgactccg ccgtcgcgga cagcgcagcg
      481 aaggcataaa gagtgcggcc agctattagc tcttggcttt tagctgctcc ggcagatcag
      541 ggacacccgc cccacggggc aaagattcgg ctagcagcga agagctagca gcaaaaggct
      601 aacgttatgg cactgagcag acagaaaaaa gcggagaagg tcggcgagct cgccaaagag
      661 ctcgagactt cgacctccgc catcatcggc accttctcca aactcaccgt cgcccaggac
      721 ttcgagctgc gcaagaccgt ccgcaatgca ggcggccgct atcgggtgct caagaacaag
      781 ctcgccggcc gcgctgccaa aggcacaaag atcgaagcgg ctcttcaggg actgaagggc
      841 gtttcctccg tcgcctacac cagcggcgat ccggtcgcgc ttgcgaaagc gctgtccacc
      901 tgggtttccg acaacgcgga gttcactttc aaactgggca tcgtcgacgg caaagtcatc
      961 aacgtcgagg aagtcaaagc cctcgcgacc atgcccggca aggaagagat tttctcgaag
     1021 ctcctctacc tcatcaatgc tccggcccag cgcctcgtca cggtcatgaa cgctgccggt
     1081 cgcgatcttg ccgttgtcat taaccagggc gtcgagaaaa acaagtttac cggcggggaa
     1141 gcagccgcca gctagttgtt cgttccgctt tccatcggga ctcgcaactt gcagatcaca
     1201 actcggaatc agctgatctt ccctctcaag gggtgcctgg tctggggcac atcggaaacc
     1261 tcgaggcggc cgaattcagc agtgcagtac ggcaaccgga gggcgcgagc tctcagggcg
     1321 caacacaaaa tttagagatt acgtggagaa caccatggca gacctgcagc agttggaaga
     1381 tcagatcgtt aacctgagcc tgctcgatgc agccgctctc gtgaaaaagc tcgaagagcg
     1441 tctcggcgtt tccgccgcgg cagccgcccc ggtcgttgtc gccggtggcg gagcagccgg
     1501 tgcggccgcc ccggctgcgg tcgagcagac cgagttcacc gtcaccctca aggatgccgg
     1561 tgccaataag atcaacacca tcaaggctgt acgcgaggtc acctccctcg gcctgaagga
     1621 agccaaggac cttgtcgatg gcgctccgaa gaccctcaag gagaacatca gcaaggaaga
     1681 cgccgaggca attaagaaga agttcgaagg cgtcgcgacc atcgaggtga agtagttcgc
     1741 tgggatcctt cccggcccgg caagcaactt gcatgccaaa gcggaacgcg ctatgattct
     1801 ctttgtgcgt tccgctcggc tgcattccag attgccggtc ccgggacgta attccgtctt
     1861 tccgatcaag cacaaaccgt aatctcgctc gtctctgacg ggctcagcag tttgcgcgcg
     1921 tgaattcggg aagaaaaatc atctccggca gattgtgccg gcgtaattgc aggccaggcg
     1981 gcggacctgc tcttacgact gcagtcagtt gtcaggcggc gcgggcgtag gttttcaaga
     2041 gcggcgggaa tcggacattc tgtcacagta cagtgcacaa ttcggttgtg gcgctcgtgg
     2101 gacttgctgt ctccgcgggc cttttgtctc ttccccgaac tttttccctc gtctggcttc
     2161 cttctttccg ccccgtgatc gatgtcccgg ctctgctctt tgctgagcgg attcgggcag
     2221 gctaaccggc agcctcgcgg gccagcagga aatcccgcga atctgcccag gcaatccctg
     2281 gcgaacatgt tcgctgggta aaacccgggt cccagcgagc ttgttcgctg ggtggaaagc
     2341 ggaagcgtaa tccccctgcg cccttccgcg caagcagaaa tcggccctgc acttcacttt
     2401 ttgtgcggcc gtaaccaaaa accgtacgac gtttcccgaa cgaggcggga tccatcgtgc
     2461 gctggcacat acggttcgac gagcagcagc atgacccggg ttgccgctct tcttttcctc
     2521 gcgtcacggg tcctgaggag taacgaatgt ccaacgagaa ccgcgcggtt cgcagccgtc
     2581 tcgacttttc caagattcca acagcgattc gcattcccaa tctcattgaa gttcagcgcc
     2641 gctcctacga gcgtttcctg cagatggaca agctgccctc ggagcgcgac gactccggcc
     2701 tgcagtcggt ctttacctcc gtctttccca ttaccgattt tcgcaacatt tcccagctgg
     2761 attttgtgga tttcgccatc ggtaactggg aatgcaagtg cggacacctc aaaggtctcc
     2821 atcacctgcg caccacctgc gtccattgcg gcgccatggt catcaccgat ccgttccatc
     2881 ccggcgacgt cctctgccag aagtgcggca cttacaacaa gaacaccccc gacttctgca
     2941 acaagtgcgg cgaccccgtc ggcctgcagc tgaaatacga ccagcaggag tgcgaagagc
     3001 gcggcatgac tttctctgcc cctctcaaag tcaccgtccg cctcaccatc tacgacaagg
     3061 atcccgaaac cggcgccaag tccattcgcg acatcaagga gcaggaagtc ttcttcggcg
     3121 acattccgct catgacgcag aacggcacct tcatcgtcaa cggcaccgag cgcgtcatcg
     3181 tctcccagct ccaccgttcg cccggcgtct tctttgagac cgccaacaat cgcacctact
     3241 tcctcggcaa gatcattcct taccgcggtt cgtgggtcga gttcgagtac gaccagaaga
     3301 acactctcta cgtccgcatc gaccgcaagc gcaaattcct cggcaccatc ttcctgcgcg
     3361 ctctcggcct caagagcgac gaggagatcc tgcgcacctt ctataccgtc gacaaaatca
     3421 atatcaaaga cggcaagctc ctctggactc tcgacgccga caagcccacc cacctgctcg
     3481 gcgccaagcc ggcccacgcc atcgtcgtca agggcgagga gatcgcgcac tccggccgca
     3541 aagtcacgcc ttccatcctc aaggctctgc gcgccgccaa agtcgcccat gtcgaagtcg
     3601 agcagtctga actcgacggc gccatgaccg ctgccgacgt ggtcgacacc tccaccggcg
     3661 aagtcttcct cgaagccaat caggaactga cctccgacaa gctgcacaaa atcatggaag
     3721 ccggtatcag caccatcgat gtcttcttcc ccgagcgcga tgacatgggc aatgtcatca
     3781 ccaacaccct gcgccgcgac tcggtccgca aacccgaaga agctctcatc gagatttacc
     3841 gcaagctgcg cccgggcgat ccgccgaccc tcgacaccgc caccgcgctc ttcgaaggca
     3901 tgttcttcga cccgcgcaag tatgacttct cgcgcgtcgg ccgcctcaag ttcaacatca
     3961 agctctacga aaatcaggac gccacctcgc tcgaccatcg caccctgacg cccgacgact
     4021 tctactccac catccgctac ctgctgaaac tccgccgcaa catcggcatg gtcgacgata
     4081 tcgatcacct cggcaaccgc cgcgtgcgcg ca
//
LOCUS       DASZYN010000275         2382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_393049, whole genome shotgun sequence.
ACCESSION   DASZYN010000275 DASZYN010000000
VERSION     DASZYN010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393049"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1582
                     /locus_tag="VHE33_07130"
     CDS             <1..1582
                     /locus_tag="VHE33_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVT97265.1"
                     /translation="AVLLVLLIACANIANLLLARAAARQSEMAMRTALGATRRRLIRQ
                     LLIESIALCCGGGAIGLSAAAGELRLLNHVLPPGTLPIPSISMDASVAWFAVGITLFT
                     GFLFGLAPAVRGSRASLSHVLRQSGRGLAGSAGARLRNSLAAAELALATILLIGAGLL
                     LQTLANLERVQLGFQPHGLLTFQLSPPPAKYPVTGGAAHLYRAVLDRLNAIPGVRGAA
                     VSSGIPFGAGNYTTHPMFTTGASVLPPSTLVPIDWRIISPGYFATMNIPLLRGRDFTD
                     ADNSTTAGVLIVSQSTAKKFWGDLDPIGRVLHPSAVPKEAITVIGVVGDVRDTALNQE
                     TPQLYYPMAARVAGLMDVVVRTSAPPDSVLPAVRQKIRELDPQLALANIRTEDEWVSN
                     SAAQPRLNSVLLGVFAAVALLIASIGIYGVLAYSVTQRTSEIGVRMALGATRPHVLRL
                     IVREGLVVSGVGIGIGLMGALALGRVLASLVFGIQVHDPSTFAVAAVTLTLVAMAASI
                     VPALRASRVDPMVALRHD"
     gene            complement(1660..2100)
                     /gene="rplK"
                     /locus_tag="VHE33_07135"
     CDS             complement(1660..2100)
                     /gene="rplK"
                     /locus_tag="VHE33_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HVT97266.1"
                     /translation="MAAPTKKIATQVKLQIEAGKATPAPPVGPALGQAQVNIMEFCKQ
                     FNARTQNKEMAGLIIPVVITVYTDRTFTFITKTPPAAVLLKKAANLAKGSGVPNKTKV
                     GTVSEAQVIEIAKQKMPDLNAASVEAAVKSIKGTARSMGIEVTA"
BASE COUNT          378 a          853 c          658 g          493 t
ORIGIN      
        1 cgcagtcctg ctcgtcctgc tcatcgcgtg cgccaatatc gccaatttgc tgctcgcccg
       61 cgctgccgcg cgccaaagcg aaatggccat gcgcacggcc ctgggcgcca cccgccgccg
      121 tctcattcgc cagctcctca ttgaaagcat cgctctttgc tgtggcggcg gagctatcgg
      181 cctttccgct gccgccggcg agctccgcct gctgaatcac gtcctgcccc ccggcacgct
      241 gcccatcccg tcgatcagca tggacgccag cgtcgcctgg ttcgccgtcg gcattaccct
      301 gttcaccgga ttcctctttg gtctcgcgcc cgccgtgcgc ggctcccgcg ccagcctgag
      361 ccacgttctg cgtcaaagcg gacgcggtct cgccggttcg gccggtgcgc gtctccgcaa
      421 ttcactcgcg gccgccgaac tggccctggc gaccatcctg ctcatcggcg ccggacttct
      481 cctgcaaacg ctggccaacc ttgagcgcgt ccagcttgga ttccagcccc acggcctgct
      541 cacgtttcag ctcagcccgc cgccggcgaa gtatcccgtg actggcggcg cagcccacct
      601 ctaccgcgcc gttctcgatc gcctcaatgc cattcccggc gtgcgcggag ctgcggtttc
      661 cagcgggatt cccttcggcg cgggcaacta tactacccac cccatgttca ccaccggagc
      721 ctcggtgctt ccaccgtcca ccctcgtgcc catcgactgg cgcatcatca gccctggcta
      781 tttcgcgacc atgaacatcc cgctcctgcg cggccgcgat ttcaccgacg ccgacaactc
      841 cacgaccgcg ggcgtgctta tcgtcagcca gtccaccgcc aaaaagttct ggggggacct
      901 tgatcccatc ggccgcgtcc tgcatccctc cgcagtgcca aaggaagcga tcaccgtcat
      961 cggtgtcgtc ggcgatgtcc gcgacaccgc gctcaatcag gaaaccccgc agctctacta
     1021 ccccatggcc gctcgcgtcg ccggtctcat ggatgtcgtc gtccgcacca gcgcccctcc
     1081 cgactcggtt ctccccgccg tccggcaaaa aatccgcgaa ctcgaccccc agctggcctt
     1141 agccaatatt cgcaccgagg acgaatgggt ttcgaacagc gccgcgcagc cacgcctcaa
     1201 cagcgttctg ctgggtgtct tcgccgcggt ggccctgctc atcgcctcca tcggcatcta
     1261 tggagtcctc gcctactccg tcacccagcg caccagcgag atcggcgttc gcatggctct
     1321 tggcgccact cgcccccatg tcctccgcct catcgtcagg gaaggtcttg tcgtctctgg
     1381 tgtcggaatt ggcatagggc tgatgggcgc cctggcgctg ggccgcgtgc tcgccagcct
     1441 cgtcttcggc atccaggtgc acgatccctc taccttcgca gtcgccgctg tcactctcac
     1501 gctggttgca atggccgctt ccatcgttcc ggcgctccgt gcctcgcgcg tcgatcccat
     1561 ggtggctctc cgccacgact aaagccttaa aaagcaaaaa ggccgaagcg ctcgtccctg
     1621 ctctcaggac gttcgcttcg gccgatccgg attagcggct taggccgtga cttcgatgcc
     1681 catcgaccgg gctgtgccct tgatggactt aacagcggct tcgacggaag cggcgttgag
     1741 gtcgggcatc ttctgcttcg cgatctcgat cacctgcgct tcgctgacgg taccgacctt
     1801 ggtcttgttg ggaacgcccg agcccttggc cagattggcg gccttcttca gcaggaccgc
     1861 cgccggaggc gtcttggtaa tgaaggtaaa ggtgcggtct gtgtagacgg tgatgacgac
     1921 cggaatgatg agtccagcca tctccttgtt ctgcgtgcga gcgttgaact gcttgcagaa
     1981 ctccatgatg ttgacctgag cctggccgag ggcggggccc acgggcggcg ccggagtggc
     2041 cttgccggct tcgatctgga gtttgacctg agttgcaatc ttcttcgtcg gtgctgccat
     2101 tttccaaaac ctctcagctg ctggctacca gtttttagct gttagctcaa agttgctact
     2161 gcaaaaatca ggcgccgtgg gcagggcata gccagcagcc aaaagctgat agctaaccgg
     2221 agctcctagg cgatcttctc gaccttgcca aaatcaagtt cgaccggagt cgaacggccg
     2281 aagatcgtaa ccatcacctt aagcgtctcg cgatcctcgt tcacctcatc gaccacgcca
     2341 ttgaagttgg cgaacggccc ctcggtaatg cggaccgatt cg
//
LOCUS       DASZYN010000276         5256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1104566, whole genome shotgun sequence.
ACCESSION   DASZYN010000276 DASZYN010000000
VERSION     DASZYN010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104566"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1457)
                     /locus_tag="VHE33_07140"
     CDS             complement(72..1457)
                     /locus_tag="VHE33_07140"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF003704.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HVT97267.1"
                     /translation="MIINLARRAHDHNWELDPIVRSLLDTDFYKLLMLQFIWKHFRQT
                     PVTFTLIDRSADQRYASLIRKEELRAQLEQVKKLRFHKSEIIWLAGNTFYGKRGIFEP
                     DFLDWLEHGFRLSEYELRAEDGRFSLAFHGAWPEATLWEIYSLAIMSELSTRAHLKQL
                     SELELDILYARAKARLWDKMERLCGVPDLHVSDFGTRRRHSFLWQEYVVTAMRDVLGE
                     SFRGTSNTYLAYKHDVEAIGTNAHELPMALAAMARDDEELQSSQYRILELWQQSYGGE
                     LLILLPDTFGTTQFLRHAPEWVANWTGQRADSKDPFVAGEEYIAWLEERGRDPRKKLF
                     IASDSLDVDEILQLHATFAGRIQEGVGREEFRRASDFLDSRRWIPEGRIRFSAGWGTL
                     LTNDFRDCNPQGGKGFDPPRLVCKLHDAEGRPAVKLSDNWAKALGPAAEIERYKRVFG
                     AEGIGEKALTT"
     gene            complement(1454..1630)
                     /locus_tag="VHE33_07145"
     CDS             complement(1454..1630)
                     /locus_tag="VHE33_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97268.1"
                     /translation="MVIERTESEGVFCLLWGEPGIRDGRVFRFLEARWMKAQGTKGFR
                     EGDHAQGRVDIVVL"
     gene            complement(1962..3440)
                     /locus_tag="VHE33_07150"
     CDS             complement(1962..3440)
                     /locus_tag="VHE33_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97269.1"
                     /translation="MMRLVLLLACLLPSLSIAQDELAGRVSEKLGSVSFATSCSGAVQ
                     AEFNRGIALLHSFAYKGAEEAFRRVADQDSSCAMAHWGIAMTHFHQLWDLPPSPADSS
                     IGLKEIGLAAGLKETSGRERGYIHALSLIFSDAGTVPYKTRALNYEAAMRDLAASDGK
                     DVEAQVFYALALISNASPSDREHGKQKQALDVLEPLFVKFPNHPGIAHYLIHACDNSE
                     LATRGLGAARAYANIAPSAPHALHMPSHIFTRLGLWDDSIASNMAARNAAHLDGDTGE
                     ELHAMDYLVYACLQSGRDREAAEVIEQLREMQNLKIGDFKVGYAATAMPIRSIVERQQ
                     WSDAEKIVDPPEPAQPHVVAIAVWARGLGFARSGQVREADRESERLRDLEGRLRGSGN
                     GYWATQVNIMEREVAAWSAQAAHQPERAGAILGSAADDEDGIEKLPVTPGPIIPAREL
                     LGELFLEQGNYAAASAAFKTALLHTPGRRGALQGAAQAARAH"
     gene            3707..5209
                     /locus_tag="VHE33_07155"
     CDS             3707..5209
                     /locus_tag="VHE33_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVT97270.1"
                     /translation="MATIAIDPRISGVTADFVSQRHQMYIDGRFVQSASGKNFPVFNP
                     ATGEVVTEVPEAEAEDVNRAVLAARRAFDDGPWPRMSPSERGRMIWKLADLLEQHLEE
                     FAEIESLDNGKPRSIARVADVPLAVDMFRYMAGWATKIGGTTLPLSAGHDFHSYTLRE
                     PIGVVAQIIPWNFPLLMAAWKLAPALACGCTIVLKLAEQTPLSGLRLAQLIDEAGFPP
                     AVVNILTGYGEGAGAPLAAHDLVDKVAFTGSTEVGKLIVHAATGNLKKVSLELGGKSP
                     AIVFPDAPIDQAVSGTASAIFFNHGQCCCAGSRLYIHEKVFDRVVEGVSQVASDIKLA
                     PGLDPSCQMGPLVSDEQYRKVVSYIESGVNEGAKIAAGGPVSGFDRGYFVRPTVLTNT
                     NPRMKVVQEEIFGPVVCAVPFSDKDVDAIIKRANDTVYGLAASVWTQNLSLAHKVARG
                     LRAGTVWVNCHNVFDASLPFGGYKQSGWGREMGEEVLHNYTEIKAVTVAL"
BASE COUNT         1011 a         1997 c         1286 g          962 t
ORIGIN      
        1 acggcagagg gtttgtgaac ccgccaggga tcgaaatcgc gaccccggtg cccttctccc
       61 acgcctcaga ttcaggtggt caacgccttc tcgccgatcc cttctgcccc aaacacccgc
      121 ttgtaccgtt cgatctccgc cgccggcccc agcgcttttg cccagttgtc cgatagcttc
      181 acggccggtc gcccctccgc atcgtgcaac ttgcacacca gccgtggcgg atcgaacccc
      241 ttcccgccct gcggattgca atcccgaaaa tcgttcgtca gcagcgtccc ccaccccgcg
      301 ctgaaacgga ttcttccctc cggaatccat ctcctgctgt ccagaaaatc cgaggctcgc
      361 cgaaattcct cccgccccac gccctcctga atccgccccg caaacgtcgc atgcagctgc
      421 agaatttcat ccacgtccag cgaatccgac gcgataaaca gcttcttccg cggatcccgg
      481 cccctctctt ccagccaggc gatatattcc tcgcccgcca caaatggatc cttgctgtcc
      541 gccctctgcc ccgtccagtt cgccacccac tccggcgcat gacgcagaaa ctgcgtcgtc
      601 ccaaacgtat ccggcaacag gatcagtagt tccccgccat agctctgctg ccacaattca
      661 aggatccggt actgcgacga ttgcagctcc tcatcgtctc ttgccatcgc cgccagcgcc
      721 atcggcagct cgtgggcatt cgtcccgatc gcctccacat cgtgcttgta agccagatac
      781 gtgttcgatg tcccgcggaa gctctccccc agcacatccc gcatcgccgt caccacatat
      841 tcctgccaca gaaaactgtg ccgccgccgc gtcccaaagt cgctcacatg cagatccggc
      901 acaccgcaca gccgctccat cttgtcccac agcctggcct tcgcccgcgc atacaaaata
      961 tccagctcca gctcgctcag ctgcttcagg tgcgcccgcg tactcagctc gctcatgatc
     1021 gccagcgagt aaatctccca cagcgttgcc tccggccacg ctccatggaa cgccagcgaa
     1081 aaacgcccgt cctccgccct tagctcatac tccgacaacc gaaacccatg ctccagccag
     1141 tccagaaaat ccggctcgaa gattccgcgc ttgccgtaaa acgtattccc cgccagccag
     1201 atgatctccg acttgtgaaa gcgcagcttc ttcacctgct ccagctgcgc ccgcaactcc
     1261 tctttccgga tcaggctggc atacctctga tccgcagacc ggtcgatcag cgtgaacgtg
     1321 accggcgtct gccgaaaatg cttccagata aactgcagca tcagcagctt gtaaaaatcc
     1381 gtatccagca gcgaccggac gatcggatcc agttcccagt tatgatcgtg cgcccgccgg
     1441 gcaagattga tgatcacagc accacgatat caacccgccc ctgagcatga tccccctcac
     1501 ggaacccctt tgtcccttgt gccttcatcc accgcgcttc caaaaaacga aaaacccggc
     1561 catcccgaat gccaggttca ccccacagca agcagaagac gccttcgctt tctgttcttt
     1621 caatcaccat tgcacccgac tccacacgaa ggtaccgctc caaccatcgc tggccccatt
     1681 ttgggactcg acccgagccc cccaaaaatc ccgaacgcca ggttcacccc acagcaagca
     1741 gaagacacct tcgcagcaaa atcatcacgg aaaaagcgcc ccatccgcgt acgaaggtaa
     1801 cccttgccgc ggccgttgat ccgtttcggg aatgatcccg cctgtacgga aagcctgcca
     1861 cccaggctcc tcttcggtcc acaagtcttt tagcggaaat tgcatccgca ttttccgatc
     1921 cggccgcaaa cggtccccag gtgaattcca caatggagga ttcaatgagc ccgcgcagcc
     1981 tgagcggctc cctgcagggc tcctcggcgt cccggtgtgt gcagcaatgc ggtcttgaac
     2041 gccgcggacg ccgcagcata gttgccttgc tcaaggaata actcccccaa caattcccga
     2101 gcgggaatga tcgggccggg cgtcaccgga agcttctcga ttccatcctc atcgtccgcc
     2161 gcgctgccca gaatcgcacc agcccgctcg ggttggtgcg cagcctgagc tgaccaggcc
     2221 gcaacttccc gctccatgat gttgacctgt gtcgcccagt acccattgcc cgatccccga
     2281 agtctcccct cgaggtcgcg cagccgctcg gattccctgt ccgcttcccg gacctgcccg
     2341 ctgcgcgcaa agccaagccc gcgcgcccaa actgcgatgg ccacaacgtg aggctgagcc
     2401 ggctccggag gatcgacgat cttctccgcg tcgctccact gctgccgttc cacgatggag
     2461 cgaatcggca tggctgtcgc cgcatatccg actttgaagt ccccgatctt caggttctgc
     2521 atttccctca gttgctcgat cacctcggcc gcctcccggt cgcgcccgct ttgcagacag
     2581 gcatacacca ggtagtccat cgcgtgaagt tcctcacccg tatcgccgtc gagatgcgct
     2641 gcatttctcg cggccatgtt ggacgcgatc gagtcatccc acagacccag ccgcgtaaag
     2701 atgtgcgatg gcatatgcag cgcatgcgga gccgaaggcg cgatgttcgc atacgccctg
     2761 gctgcgccta gtcctcgcgt cgccagctcc gaattatcgc aggcatgaat caggtagtga
     2821 gcgattcccg ggtgattcgg aaatttcaca aacagcggct ccagcacgtc cagcgcctgc
     2881 ttctgctttc catgttccct atccgatgga gaagcattgg agatcagcgc cagcgcgtaa
     2941 aacacctgcg cctcaacatc cttaccgtcc gacgccgcaa gatcgcgcat ggccgcctca
     3001 tagttcagcg ctcgcgtttt gtacggaacc gtgccggcat cgctaaagat caaactgagc
     3061 gcatgaatat agccgcgctc gcgcccggaa gtttctttga gccccgcggc cagcccaatt
     3121 tccttcagcc cgatggaact gtcggcgggc gacggaggca gatcccataa ctggtgaaag
     3181 tgcgtcatcg cgatccccca atgggccatc gcgcagctgg agtcctggtc cgccaccctt
     3241 cggaacgcct cctctgcacc tttgtaggcg aaggaatgga gcagcgcgat cccgcggttg
     3301 aattccgcct gaaccgcacc gctgcacgaa gtcgcgaacg acaccgagcc cagcttctcg
     3361 gacacgcgcc ctgccaattc gtcctgggca atcgagagcg acggcagcag gcaggcaaga
     3421 agcaggacca gtcgcatcat ctccgtctcc gttcacttcc atgatcgtcc ggccccaaat
     3481 tctacgacga acagcccccc tggtcgaggt tttgactcgg agggcatttg cgcgccgcac
     3541 gaacggggtt tgtcgatcgc acccccctcg ccaatgcaca agccccggtg gtagtgttct
     3601 ccggtgcaaa atccggcgcg ccccggcccc ggcgaaacgg cgtccccatc gaccccacct
     3661 ccttcgcccc cgccagttcg cgacccgctg cacatgaggt tctgccatgg ccaccatcgc
     3721 aatcgatccc cgtatcagcg gcgtcaccgc tgacttcgtc tctcagcgcc accagatgta
     3781 catcgacggc cgcttcgtcc agtccgcctc cggcaaaaat tttcccgtct tcaatcccgc
     3841 caccggcgaa gtcgtcactg aagtccccga agccgaagcc gaggacgtca accgcgccgt
     3901 cctcgccgcc cgccgcgcct tcgacgacgg cccctggccc cgcatgtcgc cctccgagcg
     3961 cggccgcatg atctggaagc tcgccgacct cctcgaacag catctcgaag aattcgccga
     4021 gatcgagtcc ctcgacaacg gcaagccccg cagcatcgcc cgcgtcgcgg atgtccctct
     4081 cgccgtcgac atgttccgct acatggccgg ctgggctacc aaaatcggcg gcaccaccct
     4141 tccactctcc gccggccacg acttccactc ttacaccctc cgcgaaccca tcggcgtcgt
     4201 cgcgcaaatc atcccgtgga acttccccct cctcatggcc gcctggaagc tcgctcccgc
     4261 gctcgcctgc ggctgcacca tcgtcctcaa actcgccgag cagacccccc tctccggcct
     4321 ccgcctcgcc cagctcatcg acgaggccgg attcccgccc gcagtcgtca acatcctcac
     4381 cggctatggc gaaggcgccg gcgctcctct cgccgcgcac gacctcgtcg acaaggtcgc
     4441 cttcaccggc tccaccgaag tcggcaagct catcgtccac gccgccaccg gtaatctgaa
     4501 aaaagtctcg ctcgaactcg gcggcaagtc tcccgccatc gtcttccccg acgcccccat
     4561 cgaccaggcc gtctccggca ccgccagcgc catcttcttc aaccatggcc agtgctgctg
     4621 cgccggatcc cgcctctata tccacgagaa agtcttcgac cgcgtcgtcg aaggcgtcag
     4681 ccaggtcgcc tccgacatca agctcgcccc cggcctcgac ccctcctgcc agatgggtcc
     4741 cctcgtcagc gacgagcaat accgcaaggt cgtcagctat atcgaatcag gagtgaacga
     4801 aggcgcgaag atcgccgccg gcggccccgt ttccggcttc gatcgcggct acttcgtccg
     4861 ccccacggtg ctcaccaaca ccaacccgcg catgaaagtc gtccaggaag agatcttcgg
     4921 ccccgtcgtc tgcgccgtcc ccttctccga caaagatgtc gacgccatca tcaaacgcgc
     4981 caacgacacc gtgtacggcc tcgcggcatc ggtctggacg cagaacctct cgctcgccca
     5041 caaggtcgct cgcggcctcc gggccggtac cgtctgggtc aactgccaca acgtcttcga
     5101 cgcctccctc cccttcggcg gatacaagca atccggctgg ggccgcgaga tgggcgaaga
     5161 agtcctccac aactacaccg agatcaaagc cgtcaccgta gccctctgat cccgcgaaac
     5221 ccggggacca ggcgtcccat cgcctggccc cccttg
//
LOCUS       DASZYN010000277         2029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1232462, whole genome shotgun sequence.
ACCESSION   DASZYN010000277 DASZYN010000000
VERSION     DASZYN010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232462"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VHE33_07160"
     CDS             <1..1098
                     /locus_tag="VHE33_07160"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HVT97271.1"
                     /translation="TRLGAMDFLEKPLSTDKLLLTVENALRLKRLEAENLDLRRRVGK
                     HELVWAGDAMRRVMAQIDRVAHSESRVCIYGETGTGKELVARTLHERSSRATGPFVTL
                     NCAAIPAELIESELFGHEKGSFTGATQRHAGKFEQAHRGTLFLDEIGDMPLAMQTRLL
                     RVLEESEVERIGGDKPVAVDVRVVVATHRNLEAMVDAGSFRRDLYHRVVVFPITLPPL
                     RDRVEDIPALVEYFARQVSAQNGWKPLPFAAEAIVALREYAWPGNIRELRNTVERLLL
                     LAGDSVDASTVEMALPHRSSSVARGEVPGNSGMGPLAARVAEFERATLLAELERSGGH
                     VTNAAKALGLERSHFYKKCQQLAIDVKAERA"
     gene            complement(1149..>2029)
                     /locus_tag="VHE33_07165"
     CDS             complement(1149..>2029)
                     /locus_tag="VHE33_07165"
                     /inference="COORDINATES: protein motif:HMM:NF039204.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HVT97272.1"
                     /translation="LPCLAARTVATAPTPHRHHVRHRSASSHCGTVAGHASHTVLASA
                     AAVNCRASSHPSRHTRKAAAVTLHAGERQAALSTHSQIPRARIPAMRRIRVVPPLRGS
                     LESLMRQNRRNDAEGLVRIEDDAQLEQLEMSREIVPLPASAALRVNPDLPANRRYCRP
                     WTARFLTNLARAHSARFHRSLQVNSAVRTVDFQRALIAVNGNAAPAEGDTASPHLTGA
                     AVDIGKKGLSLSEISWLRAWLLPLQTSGKIDVEEEFYQACFHITVYRSYAPPPPPRLT
                     AGRRHTSTTLLATGVQ"
BASE COUNT          372 a          577 c          721 g          359 t
ORIGIN      
        1 actcgtcttg gagccatgga ttttctggaa aagccgctct cgaccgacaa gctgctactg
       61 acggtcgaga acgccctgcg tctcaagcgg ctcgaggcag agaacctgga cctgcgccgt
      121 cgggtaggaa agcacgagct cgtatgggcc ggcgatgcga tgcggcgggt gatggcgcag
      181 atcgatcgcg tcgcgcacag cgagtcgcgg gtgtgcatct acggcgagac gggcacgggt
      241 aaagagctgg tcgcacgcac cttgcacgag cggagctccc gggccacggg gccctttgtc
      301 acactgaact gcgcggccat tccggccgag ctcatcgaga gtgagttgtt cgggcacgag
      361 aagggatctt tcaccggcgc gacgcagcgg catgcgggga aatttgagca ggcgcatcgc
      421 gggacgctct tcctggatga aatcggcgac atgccgctgg cgatgcagac gcgcctgctg
      481 cgcgtgctgg aagagtccga ggtggagcga atcggcggcg acaagccggt cgcggtcgat
      541 gtgcgcgttg tggtggcgac gcaccgcaat cttgaagcaa tggttgacgc ggggagcttt
      601 cggcgcgatt tgtatcaccg tgtcgtggtc tttcccatca ccctgccacc gttgcgggat
      661 cgggtggaag atattccggc gctggtggag tattttgctc gtcaggtcag tgcgcagaac
      721 gggtggaagc cactaccatt cgctgcggaa gcgattgtcg ctcttcggga gtatgcctgg
      781 ccgggcaata ttcgcgaatt gcgcaacaca gttgaacgct tgctgctgct ggccggcgac
      841 agcgtggacg catcaacggt agagatggcc ttgcctcatc ggtcctccag cgtggcaaga
      901 ggcgaagtcc cgggcaactc cgggatgggc cccctcgcgg cgcgagttgc ggagtttgag
      961 cgggcgacgt tgctggcgga actggagcgg agcggcggac acgtcacgaa cgcggcgaaa
     1021 gcgctcgggc tggagcgaag ccacttctac aagaagtgcc agcagttggc gattgatgtg
     1081 aaggcagagc gcgcctgacg cactgatatt tgcagcaatt tgggagagcg gtttagcctg
     1141 gggcggctct attgcacgcc ggtggccagc aacgtcgtgc tggtgtgacg acgtcctgcc
     1201 gtcagacgcg gaggcggagg cggtgcatag ctccgataaa ctgtgatgtg gaagcaggcc
     1261 tggtagaact cctcttccac gtcgattttg cctgaggttt gcagcggcag cagccaggca
     1321 cgcagccacg aaatctcaga gaggcttagc cccttcttgc cgatgtcgac agcggccccg
     1381 gtcagatgcg gagaagctgt atcgccctcg gccggcgcgg cattgccgtt cactgcgatc
     1441 agcgcccgct ggaaatccac cgtacgcacg gctgagttga cctgcagcga gcggtggaaa
     1501 cgcgcagagt gggcgcgtgc gagattcgtc aggaagcgcg cggtccaggg acggcaatag
     1561 cgccgattag ccgggagatc gggattaaca cgcagcgcag cgctggccgg aagcggcacg
     1621 atttcgcgag acatttcgag ctgctccagc tgtgcgtcgt cttcaatacg gaccaggccc
     1681 tcggcatcgt tgcgccggtt ctgccgcatc agcgattcca gcgacccgcg cagcggcgga
     1741 acaacgcgaa tgcggcgcat agccgggatt cttgctcgtg ggatctgcga atgcgttgaa
     1801 agggccgcct gccgctcgcc ggcgtgcagg gtcacggccg ctgctttcct ggtatgccgg
     1861 gagggatggc tcgacgcccg gcagttgacc gcggccgcac tggccagaac cgtgtgagag
     1921 gcatgccccg caacagtgcc gcagtgcgac gaggcactcc ggtgacggac gtggtgacgg
     1981 tgaggcgtcg gagcggtggc aactgtcctg gctgccaaac aggggagcg
//
LOCUS       DASZYN010000278         3458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_12582, whole genome shotgun sequence.
ACCESSION   DASZYN010000278 DASZYN010000000
VERSION     DASZYN010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12582"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VHE33_07170"
     CDS             complement(<1..710)
                     /locus_tag="VHE33_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97273.1"
                     /translation="MTHNSGHQTPDWTANLIRFLCPRTGSASISTLALLALLWVLTAS
                     IHAQTASFSGSTTVFDSSHFAAAEGITTDASGNLFIAATPDHSVWNVYEMQRISPGVY
                     SAPVALPTPSGGYGCSGIMYCLRNVAVDPSRNVWVAAWGTGKVYKVPYNTATHSYTAS
                     ASLISGPSPSGWQKPWGIASDAAGNIFVGDETANSIAKITGSTATWINTTSVHQPGGM
                     VFDSGGNLLILDGNVARVV"
     gene            1032..1772
                     /locus_tag="VHE33_07175"
     CDS             1032..1772
                     /locus_tag="VHE33_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HVT97274.1"
                     /translation="MKPAELRARILRAQPPLGGQTVHVWRTALSTITPLADDMRALLS
                     ADEHDRADRFRFPEHRNAFIAGRAVLRMVLGSYCGCAPEKLHFSYGEQQKPGVDEIES
                     PLARTVSFNLSHSADELQIAVAAQGALGIDVEDPGREFDVDALIADCLMEDEARALTH
                     LSSEQRKTAFIRYWVHKEAFLKCVGTGFSVSPKEIHVAFDGGRSEMRCSNLMANNVLF
                     GRNLHCAPGHLAAIATAEREYELLSIVL"
     gene            complement(1809..>3458)
                     /locus_tag="VHE33_07180"
     CDS             complement(1809..>3458)
                     /locus_tag="VHE33_07180"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT97275.1"
                     /translation="SVRSGERFAWQDADAIVNLSSAADLNLLWERIDAAGLHPEGLIC
                     LSGAGDAANSAYDTILQILQTAGARRRFLRRFEFITTSAESVSGEPLTHPASGELHGL
                     ARTIPAEFDSSECRCIDVELPEHDLQPVVQQLLDELSTSGANLTIAYRGGVRWQKGWS
                     PALLKGSSESPFRRGGVYLITGGVGGIGYVVAKHLLQNHAARVTLVGRTTVPDHNDWE
                     SWILSHGEGDPISRRIRRLQDLEQAGGEVLFQAADVADRDAMRLVLEQTQQQFGPLNG
                     VIHAAGVPGGNRLLLQPLDEARQIRRPKIDGSRVIAELLQGSRIDFLLFCSSISASVP
                     AASQSAYAAANAFQNAFAEYCRSALHVPAIAIGFDGWREVGMLADTHVPVGLESYYEE
                     RMRSALTNSEGIEVIHRALTQWRGSQILVSAMDFDRLSTAAHAFAPIAPRYDDHVAAD
                     AQGRELKVIIEIWKDLLGVDSISPSDNFFTLGGHSLMGTMMIARIRDRLGVTLSLRDV
                     FEERTPEGIAALVCSRAEEKTPVEQVAQPAPSDDKREVFEF"
BASE COUNT          718 a          935 c         1114 g          691 t
ORIGIN      
        1 acgacgcgag cgacgttgcc gtccaggatc agcagattgc cgccggagtc gaaaaccatg
       61 ccgccgggct ggtggacact ggtggtgttg atccaggttg cggtgctgcc ggtgattttg
      121 gcgatggagt tggcggtttc atcgccaacg aagatgtttc ccgccgcgtc ggaagcgatc
      181 ccccagggct tttgccagcc ggagggagag ggtccggaaa tgagagacgc gctggcggta
      241 taggaatgag tcgcggtgtt atacggcact ttgtagactt tccccgtacc ccaggcagcg
      301 acccacacgt tgcggctggg atcgacggcg acatttctca ggcagtacat aatccccgag
      361 caaccgtagc cgccggaagg ggtcggtaga gcgacgggag cgctgtagac gcccgggctg
      421 atgcgctgca tttcgtagac gttccaaacg gagtggtctg gcgtggccgc gatgaacaag
      481 ttgccgctcg cgtctgtggt gatgccttct gcagctgcga agtgcgaaga gtcgaacaca
      541 gtggtgctgc cgctgaagga ggcggtttgc gcatggatgg atgcggtgag aacccacaga
      601 agagcgagca gagccagagt ggaaatgctc gcggaccccg ttcgcggaca aaggaaacga
      661 atgaggttcg ccgtccagtc gggggtctgg tgcccggagt tgtgagtcat cgagagatcc
      721 tggtgaaggc agtggaacaa gttgaaactt cgacccggaa cggcggggaa aagcggatcg
      781 aacaggagct ttaatacagg gactgcaagg tactttgaag ggattctacg gggccaatct
      841 gctggtgaaa tccccaaatg gattcggcat cggcaaactc tgggaacctg gggtaaccac
      901 ttgccatctg ctgcaatatg tgtgagatac gataggcacc agggtggaaa tccggtgagc
      961 ctgtcgattt cggacgagat ggtcgggaac cccgatgcgg gggcctttgg gtgtcgcgtt
     1021 tggaacatcg tatgaagcca gcagaactga gggcaagaat tctgagagcg cagccgcctc
     1081 tgggtgggca aacggtccac gtgtggcgca ccgcgctttc aacgattact ccgttggcgg
     1141 acgacatgag ggcgctgctg tctgccgacg aacatgatcg cgcggacaga tttcggtttc
     1201 cggagcatcg caatgcgttt atcgcggggc gcgctgtgtt gcggatggtt ttgggcagct
     1261 attgcggatg cgcgccggag aaactgcact tcagctatgg cgaacaacag aaacccgggg
     1321 tcgatgaaat cgagagtccc ctggcgcgga cagtttcctt caatctttcg cattcggccg
     1381 acgaactgca gatcgcggtc gcagcgcagg gagcgttggg aatcgatgtt gaggatccag
     1441 ggcgggaatt cgatgtcgac gctctaattg cggattgcct gatggaggac gaggcgcgag
     1501 cgctgaccca tctgagctcc gagcagcgga agaccgcgtt catccgctat tgggtccaca
     1561 aggaagcttt cctgaagtgt gtggggacgg gattctcggt gtcgcccaaa gagattcacg
     1621 ttgctttcga cggcgggcga tcagagatgc ggtgctcgaa tttgatggcg aacaatgttc
     1681 tgttcggccg caatttgcac tgtgcgcccg gacatctggc agcgatcgcg acggccgagc
     1741 gagaatacga gcttctgtcc atcgttttgt gaagcggttt gaaggcggaa cctgctacgc
     1801 gaatcgcatc aaaattcgaa cacttcgcgt ttgtcgtcgg agggtgcggg ctgtgcaacc
     1861 tgctcgaccg gtgtcttctc ctcagctcgc gaacagacga gagcggcgat tccttcgggg
     1921 gttcgctcct caaagacatc gcgcagtgag agtgtgacgc ccagacgatc gcggatgcgg
     1981 gcgatcatca tggtgcccat gagcgagtgg ccacccaaag tgaaaaaatt gtcggagggg
     2041 ctgatggagt caacgcccag caagtccttc caaatctcga taatgacttt tagttccctg
     2101 ccctgggcgt ctgcggcaac gtggtcgtcg tacctcggcg cgattggtgc gaatgcatga
     2161 gccgcagttg acaggcgatc gaaatccatc gcgctgacaa gaatctgcga tccgcgccat
     2221 tgcgtcaggg cacgatggat gacctctatc ccttcggagt tggtcagggc gctgcgcatt
     2281 ctttcctcgt agtacgactc caggcctacc ggcacatggg tgtccgccag catgccgacc
     2341 tcacgccagc cgtcgaatcc gatggcgatc gcgggaacat ggagcgcgct gcggcagtat
     2401 tccgcgaagg cgttctgaaa agcgtttgca gctgcataag cgctttgcga tgcggccgga
     2461 acggaggcgc tgattgatga gcagaacaac agaaagtcaa tcctgctgcc ctggagcaat
     2521 tcggcgatca cgcgtgaccc gtcaatcttt ggcctgcgaa tctgacgggc ctcatcgaga
     2581 ggctggagca ggagtctgtt tccacccgga acaccagcgg cgtgaatgac gccgttcagc
     2641 ggaccgaact gttgctgggt ttgttcgaga acgagccgca tcgcatcacg gtcggcaacg
     2701 tctgcggcct ggaagagtac ctctccgccc gcttgctcaa ggtcctggag tcttcgaatg
     2761 cgacgggata tggggtcgcc ttctccatga ctgagaatcc aggactccca atcgttatga
     2821 tccggcacag tagtccggcc gacgagcgtc acgcgggcag catgattctg cagcagatgc
     2881 ttcgctacga cataaccgat gccacctacg ccgccggtga tcaggtaaac gccgcctctc
     2941 cggaagggcg attcagacga tcccttcaga agagctgggc tccatccctt ctgccatcgc
     3001 acaccacccc ggtatgcaat ggtgagattc gcgccactgg tggagagctc atcgagtagt
     3061 tgttgaacaa ccggctgaag atcgtgctcg ggcaactcta cgtcgatgca gcggcactca
     3121 gaactatcga attctgcggg aatggtgcga gcgagaccat gcagttcccc gctcgcggga
     3181 tgagtgaggg gctctccgct aacgctttcg gccgacgtgg tgatgaattc gaacctgcga
     3241 agaaatcgcc gccgagcgcc cgcggtctgg aggatttgaa ggatcgtgtc atacgccgag
     3301 ttcgctgcgt ctccggctcc gctcaggcat atcagacctt cagggtgcag cccggcagcg
     3361 tcgatccgtt cccaaagcag gttcagatcg gccgcactgg acaagttcac aatggcgtct
     3421 gcgtcctgcc aggcaaagcg ctcgccggag cgcaccga
//
LOCUS       DASZYN010000279         4424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_393336, whole genome shotgun sequence.
ACCESSION   DASZYN010000279 DASZYN010000000
VERSION     DASZYN010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393336"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3828)
                     /locus_tag="VHE33_07185"
     CDS             complement(<1..3828)
                     /locus_tag="VHE33_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97276.1"
                     /translation="MSSPSHINIALAAAHGITDRLIATWNPDLPRDPRVLVPVLVEAL
                     VVRQEGGQWADTSLKQTPESGDAVPTESLLPKPFALDSGARPKGVYLHWALPDALTKG
                     TQTPNADASADTSNAAAVFPAVPDRWLVVRLSPSTTHPDRRAVKGWILQAHDKDAIRH
                     DLDGWRDPGTPPEGLQKPLTALGWGDISWAGYFDNTENRLAMYDDLSGVKEGPLAYLV
                     CGWYSDPQLDPLGPANVASLSQFNAAMQKLGWALPDIDLKQPKSSSSDSVLTARMVGL
                     PVNRIRTSVQLNNFASSSDTPAGGLVGFDPTSGTYTTDGSWWPQMCLLHGAVVGIGWP
                     GIGWEGNPTGVWNPKTLDPSIGQEEPGGPPDPAGIRVAMGNTVTEAMARLVATGNNRP
                     DETRILEAFQLNALTVLNDADGPAKVDSLLHGRQFGSVPGEPYTETIWQPPTSVNQAP
                     PAHPPKPGTGTFSRYKDAATKGPFGYGVVNPLGAVTKSVLTPATAGSRQIQQESLLLK
                     GGLASAINQIGLNHPIPEPQPGKWITVQRTGPRLFHPSDPCLLIAGGKASFKHKLGAQ
                     SKDGMLYCRLTNDYADELSSPTVNIPGLTPQRPYARGADLLERGIENGSVPGECTGLL
                     NELAVLDPGSSLHAARSFATSANLNATQTAALARSFLVEQTVWHATRDPRVDHGPLIA
                     KSGFSGRLPSAIAINLPARPWNPIRLDWSVEFIPSANGAADWALGEIDYAPQGVNLGT
                     PISFTGSSILTQGANNIVASAITKALEQAASAGGSGIRQPGILIKYVSTIAQKHVDAF
                     SKIVSTQAAQSAGGASTGVPSVDRLPLDDIASALSDMDVLTGGLDGFTSQLRGGYRGD
                     GSTVPPSSAPDPSPFFPWRAGFLRVTALRLVDGFGQFIDLLESNQQFQTAQIIKSDPM
                     AVDGHDDLALLPPRFTSPARLLFEFVDAKDSGQRAVSDPQGGNPISPVCGYLMPNHLD
                     GALEFFDTDGTNLGFVRPQDDATVMWEEAPGQPTTAGHDPGAAIPNPHAAEIAKALVR
                     WSIADAGVPGERDDALQAILRVIDATLWTVDPFGHIGDEHLALLVGHPVAIIRARITL
                     QLREPIRPDLANTTLVPLRLGALAHWQDGLFGYFVNDDYTRLYCSDAAAAGLARAIGP
                     GTGFLGAINSVPDQLTNFAADTGTTPVTHPYIDTSGIVMIRPNQTIDLTLLLEPHSVV
                     HATAGLLPRKEIGLRRDWTDAALSKLSPTFRFGPVLVDPQRIRMPVPIDLNGTWSWDH
                     RADINTWQEQEVTN"
     gene            complement(3825..>4424)
                     /locus_tag="VHE33_07190"
     CDS             complement(3825..>4424)
                     /locus_tag="VHE33_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97277.1"
                     /translation="ADIVTGFLLRSAAVSGWPHMDVRAFNTIIEHGKDVSQIPANQML
                     PLLRLERLSPSILVALFQGIPKLVWCEEPHHGVQFAVSETLTIPARDATGVAQTTVNP
                     IPVPLRNNARRVIDVAALQALVHQKEGNPAPANGWPAGTGAASFAISVLNPPWRQRFQ
                     GAGGDLLHSGDGRFNYFTAIAASVETPQLKLEVKKLVTP"
BASE COUNT          859 a         1319 c         1410 g          836 t
ORIGIN      
        1 gttggtcact tcctgttcct gccacgtatt aatgtcggcg cgatggtccc agctccaggt
       61 gccgttcagg tcgatgggta ccggcatacg gatccgctgc ggatcgacca gaacggggcc
      121 gaatcggaag gtgggcgaga gtttgctcag agctgcatcg gtccagtcgc ggcgcagccc
      181 aatctctttg cgcggcagca gaccggcagt tgcgtggact accgaatggg gttcgagcag
      241 caatgtgagg tcgatagtct ggttcgggcg gatcatgacg atgccgctcg tatcgatata
      301 aggatgcgtt accggtgttg tgcccgtatc ggcggcgaaa ttcgtcaact ggtccggcac
      361 agaattgatt gcgcccagga agccggtgcc agggccaatc gcgcgggcga ggcctgcggc
      421 cgcggcatcg gaacagtaca ggcgggtgta gtcgtcgttg acgaaatatc cgaagagccc
      481 atcctgccag tgcgcgaggg ctccaaggcg caatggaacc agggttgtat tcgcgaggtc
      541 gggccgaatg ggttcgcgca gttgcagcgt gatgcgcgcg cgaatgatgg ccacgggatg
      601 ccccacgagc agagcgaggt gctcgtcgcc gatgtggccg aagggatcga cggtccacag
      661 ggtcgcatca atgacacgaa ggatcgcctg cagcgcgtca tcgcgctcgc cgggaactcc
      721 cgcgtcggca atgctccagc gcacgagcgc ctttgcaatt tctgccgcgt gcgggttcgg
      781 aattgccgcg ccgggatcgt gacccgcggt ggttggctgg cctggcgctt cttcccacat
      841 caccgtcgcg tcatcctggg gacgaacaaa gccgagattg gtaccgtcgg tgtcgaagaa
      901 ctcgagcgcg ccgtcgagat ggttcggcat caggtatccg cagacgggac tgatcggatt
      961 cccgccctgg ggatcgctga cggcgcgctg gcctgaatcc tttgcgtcaa caaattcgaa
     1021 caacaggcgc gcaggagaag tgaagcgcgg cggaagaagt gccagatcat catgaccgtc
     1081 aacggccatg ggatcggatt tgatgatctg cgccgtctgg aactgctgat tggactcgag
     1141 cagatcgata aactgaccga agccgtcgac caggcgcagc gcggtcaccc gcaaaaatcc
     1201 ggcacgccac ggaaagaacg ggctgggatc gggagcactc gacggcggta ccgtgcttcc
     1261 atcgccgcgg tatccgcccc gcaattgaga ggtgaatccg tcgagcccgc ctgtcaaaac
     1321 atccatgtcg gagagagccg aagcaatgtc atcgagcggc aggcgatcga cggagggcac
     1381 accggtcgac gcgccacccg cgctctgcgc tgcctgggtg ctgacaatct tgctaaacgc
     1441 gtccacgtgc ttttgcgcga tggtcgagac gtacttgatc agaattcccg gctgccgaat
     1501 gccgctccct ccggcgctgg ccgcttgttc gagcgccttc gtaatcgcgc tggccacaat
     1561 attgttcgcg ccctgcgtga ggatggaact gccggtgaaa gagatgggcg tacctaaatt
     1621 cacaccctgg ggcgcgtagt cgatttctcc cagcgcccag tcggcagcgc cattcgctga
     1681 aggaatgaac tccacgctcc agtcgagacg aatcggattc cacggacgag cgggaagatt
     1741 gatcgcgatg gccgacggca atcgtccgct gaagcccgac ttcgcgatga gcggtccgtg
     1801 atctacccgt ggatcacgcg tcgcatgcca taccgtttgt tccaccagga acgaacgagc
     1861 cagggccgct gtttgcgtcg cgttcagatt tgccgacgtg gcaaagctgc gtgcagcgtg
     1921 cagcgagctg ccgggatcaa gaacggcaag ctcgttcaac aggccggtgc attcacctgg
     1981 aacgctgccg ttttcaatcc ctcgctccag cagatcggcg ccgcgcgcgt aaggccgctg
     2041 cggagtaagg ccaggaatgt tgacagtcgg cgaactgagt tcgtcggcat agtcattcgt
     2101 gagcctgcag tacagcatgc cgtctttgga ctgggccccc agtttgtgtt tgaacgatgc
     2161 cttgccgccc gcgatcagca ggcagggatc agagggatgg aagagccggg gaccggttct
     2221 ctggaccgtg atccattttc caggctgcgg ctccgggatc ggatggttca gcccgatctg
     2281 gttgatggcc gatgccagtc cacccttgag cagaagtgat tcttgctgta tctggcgtga
     2341 tccggcggtg gccggcgtga gaaccgattt tgtcacagca ccgagcggat tcacgacgcc
     2401 gtatccaaaa ggacccttcg tggctgcgtc cttgtagcgc gagaacgtac ccgttcccgg
     2461 cttcggtgga tgcgccggcg gagcctgatt cacggatgtg ggcggctgcc agatcgtttc
     2521 ggtgtagggc tcacctggga cagacccaaa ctgacgccca tgcagaaggg aatcgacttt
     2581 cgcgggaccg tctgcatcat tgagcacggt cagcgcgttg agttgaaacg cttccagaat
     2641 ccgcgtttca tccggacgat tatttcccgt cgcaaccaga cgcgccatgg cctcggtgac
     2701 ggtgttcccc atagcaacgc gaatacccgc aggatccggc gggccgcccg gttcctcctg
     2761 tccgatggag ggatccagcg tctttgggtt ccacacacca gtgggattgc cttcccatcc
     2821 gatgcctggc catccgatac caaccacggc gccgtgcaac aggcacatct gcggccacca
     2881 cgatccatcg gtggtgtagg tgccgctggt gggatcgaag ccaaccaacc cgccagccgg
     2941 agtatcgctg cttgacgcaa agttgttgag ctgaacgctg gttcgaatcc gattcaccgg
     3001 cagcccgacc atgcgtgcgg tcagcaccga atcgctcgat gaggatttgg gctgcttgag
     3061 atcgatatcc ggcaaagccc agcccagctt ctgcatcgcc gcattgaact gcgatagcga
     3121 agcgacattg gccggcccta gcggatcgag ttgcggatcg gagtaccagc cgcagacaag
     3181 ataggcaagc ggaccttctt tgacgcccga cagatcgtcg tacatcgcca ggcgattctc
     3241 ggtattgtcg aaatatccgg cccaggaaat atccccccat ccgagtgcag tgagcggctt
     3301 ctgcaaaccc tcaggcggtg tgcccggatc cctccagccg tcgaggtcgt gacggatggc
     3361 atccttatcg tgcgcctgga gaatccatcc tttgacggct cgccgatccg gatgcgttgt
     3421 ggacggcgaa aggcgcacca ccagccatcg atccggcaca gccgggaaaa cagctgcggc
     3481 gttgctggtg tcggcggatg catccgcgtt cggagtctgc gtgcctttcg taagcgcgtc
     3541 cggcaacgcc cagtgcagat aaacaccctt cggacgagcg ccggagtcca gtgcaaacgg
     3601 cttcggcaaa agagattccg tcggtactgc atcgccgctt tcgggagtct gcttcaacga
     3661 ggtgtcggcc cactgtccgc cctcctggcg aaccacgagc gcttccacca ggaccggaac
     3721 cagaacccgc ggatcgcgcg gcagatccgg attccacgtg gcaatcaggc ggtcagtaat
     3781 gccatgggcc gcggcgagcg cgatgttgat gtgcgaagga ctgctcatgg tgtcaccagc
     3841 ttctttactt cgagcttcag ctgcggcgtc tcgacgctgg cggcaatcgc agtgaagtag
     3901 ttgaatcggc cgtcgccgga gtgcagcagg tcgccgcccg cgccctgaaa ccgttgccgc
     3961 cagggcggat tgagcacgga aatagcaaaa ctggcggcgc cggttccagc cggccagccg
     4021 ttggccggag ccggattgcc ttccttctgg tggacgagtg cctgcaaagc cgcgacatcg
     4081 atgacgcgcc ttgcgttatt ccgcagagga accgggatgg gattcacggt ggtctgcgcg
     4141 actcccgtgg cgtcgcgggc cggaatggtg agcgtctcgg aaacggcaaa ctgcacgcca
     4201 tgatgcggct cttcgcacca cacgagtttg ggaattccct gaaagagcgc gacgagaata
     4261 ctgggcgaga gccgctccag gcgcaaaagg ggaagcatct gattcgccgg aatctgactc
     4321 acgtctttgc cgtgctcgat gattgtgttg aatgcgcgga catccatgtg gggccatccg
     4381 ctcacggctg ccgaacgcaa caggaagcca gtcacgatgt ccgc
//
LOCUS       DASZYN010000280         1666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_846063, whole genome shotgun sequence.
ACCESSION   DASZYN010000280 DASZYN010000000
VERSION     DASZYN010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846063"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VHE33_07195"
     CDS             complement(<1..363)
                     /locus_tag="VHE33_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT97278.1"
                     /translation="MDSVVTFLMTQNPDAAIAFYRDKLGLRFLRDDGFALVFDMSGVM
                     LRIAKTKSFTPAQATVLGWESRDIASTLKQLAQRGIAFEHYPNMGQDADGICTFPNGD
                     KVAWFKDPDGNVLSISQHV"
     gene            513..>1666
                     /gene="dnaE"
                     /locus_tag="VHE33_07200"
     CDS             513..>1666
                     /gene="dnaE"
                     /locus_tag="VHE33_07200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HVT97279.1"
                     /translation="MPEFTHLHLHTDYSLLDGACDVEKLVQRVASIGQKSVAITDHGN
                     IYGAVHFFDAAKKHGVHPILGCELYVCKKEDHREDPQGDDYNHLLVLAENEEGYRNLV
                     RITSEASLHGFYRKPRISKKYLAEHAKGLIGFSGCLAGELCENLMADKYDAAKATAAQ
                     YQDIFGKGNFFLEIQDQGLELEKKIQADLFRLEKELEIPLVATNDSHYLCEDDAHAHE
                     VLLCVQTLASINDPKRFKFDSDQFYVKSAAEMERLFKHAPEVVKRTMEFAERCHLKLN
                     KVDNPFPEFTVPPGHTIDSYFEEVCRQGLKKRLETAVRELSARGVLRTSVAEYQSRLD
                     FEIGIIKQMQYSGYFLIVWDFIKYARDHGIPVGPGRGSATGSLVAYAMEIT"
BASE COUNT          348 a          442 c          541 g          335 t
ORIGIN      
        1 gacgtgctgc gagatcgaca gcacgttgcc gtcgggatct ttgaaccagg cgaccttgtc
       61 tccgttggga aatgtgcaga tgccatcggc atcctgaccc atgttcgggt aatgctcgaa
      121 ggcgatgcct ctttgcgcca gttgtttcag ggttgaggcg atgtccctcg actcccagcc
      181 gaggacggtg gcttgtgcgg gcgtaaaact tttcgttttc gcaatccgca gcatcacgcc
      241 gctcatgtcg aagaccaggg caaagccgtc gtcgcgcagg aaccgtagac cgagcttgtc
      301 gcgatagaag gcgatggccg cgtcaggatt ctgcgtcatt agaaacgtga cgacgctgtc
      361 catggagacc tccgcgtgca gaacatcgta cacgctggac gtgaacccgc aacagcgcat
      421 cggggcggag ttttgtcccg ccttttcctc aaacggccgt cgcgatcctc ggagggctgc
      481 tgttgcgcga acgcctaaaa tagaagctga acatgccgga atttacccat ctgcatctgc
      541 atacggacta ctcgctgctg gacggcgcct gcgacgtgga gaagctcgtg cagcgtgttg
      601 ccagcatcgg gcagaagtcg gtcgcgatca ccgatcacgg caatatctac ggcgcggtgc
      661 atttcttcga cgcggcgaag aagcatggag tacacccgat tctgggatgc gagctgtacg
      721 tgtgcaagaa ggaggatcac cgcgaggatc cgcagggcga cgattacaac cacctgctgg
      781 tgctggcgga gaacgaagag gggtatcgga atctcgttcg catcacgtcg gaggcgtcgc
      841 tgcacggttt ctaccggaag ccgcggatca gcaagaagta cctggctgag catgcgaagg
      901 ggctgattgg attctctggc tgtctggcgg gggagttgtg cgagaacctg atggccgaca
      961 agtacgacgc ggcgaaggcg acggcggctc agtaccagga catctttggc aagggaaact
     1021 tttttctgga gattcaggac caggggctgg agctggagaa gaagattcag gcggatctgt
     1081 tccggctgga gaaggaattg gagattccgc tggtggccac gaacgacagc cactatttgt
     1141 gcgaagacga cgcgcacgcg cacgaggtgc tgctgtgcgt gcagaccctg gcgtcgatca
     1201 acgatccgaa gcggttcaag tttgactccg atcagttcta tgtgaagtcg gcagcggaga
     1261 tggagcggct gttcaaacat gctcccgaag tcgtgaagcg gacgatggag ttcgccgagc
     1321 gctgccatct gaagctgaat aaggtcgaca atccgtttcc ggagttcacg gttccaccgg
     1381 gacacacgat cgacagctat tttgaggaag tttgccgcca gggactcaag aagcgcctgg
     1441 agacggcggt gcgtgagttg tcggcccgcg gggttctgcg cacctcggta gccgagtacc
     1501 agagccggct ggatttcgag atcggcatca ttaagcagat gcagtactcg gggtatttcc
     1561 tgatcgtgtg ggacttcatc aagtacgcac gcgaccatgg aattcccgtt ggccccggcc
     1621 gcggctcggc gaccggctcg ctggtggcct atgcgatgga gatcac
//
LOCUS       DASZYN010000281         5320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_778653, whole genome shotgun sequence.
ACCESSION   DASZYN010000281 DASZYN010000000
VERSION     DASZYN010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778653"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /gene="sufB"
                     /locus_tag="VHE33_07205"
     CDS             <1..1466
                     /gene="sufB"
                     /locus_tag="VHE33_07205"
                     /inference="COORDINATES: protein motif:HMM:NF008773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HVT97280.1"
                     /translation="EPMSTDMNVVHEMASKEYEWGFVTQIEEDRIPKGLNEDVIRLIS
                     TKKNEPEFMLEWRLNAYRYWASQEKANAEPQWANVTFPPINYQDIAYYSAPKQKKQLT
                     SLDEVDPEILRTYEKLGIPLAEQKLLANVAVDAVFDSVSVATTFKAKLAEVGVIFCSF
                     SEAVKNHPDLVKKYLGSVVPHTDNFYAALNAAVFSDGSFVYVPKGVRCPMELSTYFRI
                     NAAETGQFERTLIVAEEGAYVSYLEGCTAPMRDENQLHAAVVELVALDNAQIRYSTVQ
                     NWYPGDKNGKGGIYNFVTKRGKCLGRNSKISWTQVETGSAITWKYPSCILQGDNSVGE
                     FYSVALTNNYQQADTGTKMIHLGKNTRSTIVSKGISAGHGQNTYRGMVKIMKGAEGAR
                     NYTQCDSLLIGDKCGAHTFPYIEVKNSSARMEHEAATSKIGEDQLFYLQQRGLKTEDA
                     VSMIVNGFCKQVFKELPMEFAVEAQKLLAVSLEGSVG"
     gene            1712..2482
                     /gene="sufC"
                     /locus_tag="VHE33_07210"
     CDS             1712..2482
                     /gene="sufC"
                     /locus_tag="VHE33_07210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HVT97281.1"
                     /translation="MSLLEIKNLHVNVDGNEILKGIDLTLNAGEVHSIMGPNGSGKST
                     LAQVLARRDAYNVTQGSITFDGKDLLEMKPEEAACEGVFLAFQYPVEIPGIGNTYFLR
                     SALNAQRKYRGEDEMDAMDFLPMFREKLKLLEMDDKLMNRSLNEGFSGGEKKRNEILQ
                     MAVLEPKLAILDETDSGLDIDALRIVANGVNSLRSPDRAMLVVTHYQRLLNYIVPDFV
                     HVLVDGRIVHSGGKELALELEEKGYGWTETPQPVGARS"
     gene            2494..3867
                     /gene="sufD"
                     /locus_tag="VHE33_07215"
     CDS             2494..3867
                     /gene="sufD"
                     /locus_tag="VHE33_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HVT97282.1"
                     /translation="MAVSHIDSWLENFTDFEKKAAGRTLPWLHRLREDAFARFCEVGF
                     PTTKDEDWRFTNVSGIAKTVFRPASTAKLASGDSAAWRVDDVAATLVFVNGHFDAQLS
                     NIGNLPKGVAVASLRERIASHPEEVQQHLGRYADTQRDAFVALNTAFIGDGAYVHVAR
                     GVVLEAPIYLLYLSTKENAPTMTHPRNLIVAEDQAQVAVIEDYASVGGDSAVLSNAVT
                     ELIAGESTVAQHYLIEREHLKAWNISTLRIQQGRSANVASHSLLLGGGLVRNNVHPVL
                     AGEGGECLINGLFLGTGAQHLDNYMHVEHAAPHCGSRQFYNGILDGHSHGVFHGRIVV
                     HKDAQKTDAKQTNRNLLLSDDAQIDTKPQLEIYADDVKCTHGATIGQIEENALFYLRS
                     RGISEQEAKKLLLMAFAEECVERMQSSVAREHIERLVNEHLLFTEGVVPPPAAERVSE
                     RTVEEVG"
     gene            3864..5123
                     /locus_tag="VHE33_07220"
     CDS             3864..5123
                     /locus_tag="VHE33_07220"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HVT97283.1"
                     /translation="MSAATAVPIREKAASFDVDRIRRDFPILHTKVHGHPLVYLDNAA
                     TSQKPQAVIDALVRYYQGENANIHRGVHYLSELATAEFEKARETVRAFINAARSSEII
                     FTRGTTESLNLVAQTYGKSNLRAGDEVLITAMEHHSNIVPWQMLCEEKGAKLQVAPIN
                     DQGELLFAEYEKRIGPKTKIVAVGHVSNALGTINPLRQMIAAAHAQGAIVVVDGAQAV
                     PHLRVDVQALDADFYAFSGHKMYGPTGIGVLYGKQALLEAMPPYQGGGDMISSVTFEK
                     TTYNKVPHKFEAGTPDMAGVAGLMAAIEYMNAIGIDRIAAHEHDLLVYATEVIQSVPG
                     VRLIGTAAEKAGVLSFVLDEVHPHDIGTILDQQGIAVRTGHHCAQPVMERFSIPATVR
                     ASFAVYNTKAEVDALARGIRKVREVFA"
     gene            5123..>5320
                     /locus_tag="VHE33_07225"
     CDS             5123..>5320
                     /locus_tag="VHE33_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HVT97284.1"
                     /translation="MPELRDLYQEVILEHSKAPRNYKALESANHKAEGYNPLCGDRFT
                     VYLQMDGDTIREIGFQGAGCAI"
BASE COUNT         1172 a         1743 c         1493 g          912 t
ORIGIN      
        1 gagagccaat gagcactgac atgaatgtcg tccacgaaat ggccagcaag gaatacgagt
       61 ggggcttcgt cacccagatc gaagaagacc ggattcccaa gggcctcaac gaggacgtca
      121 tccgcctcat ctccaccaag aagaacgagc cggagttcat gctcgaatgg cgcctgaacg
      181 cttatcgcta ctgggctagc caggagaagg cgaacgccga gccgcaatgg gcgaacgtta
      241 cctttccgcc catcaattat caggacattg cctactactc cgcccccaaa cagaaaaagc
      301 agctgaccag cctggacgaa gtcgaccctg agattctccg tacctacgag aagctcggca
      361 ttcccctcgc cgaacaaaag ctcctcgcca acgtcgccgt cgatgccgtg ttcgactcgg
      421 tgagcgtcgc caccacgttc aaggccaagc tggccgaggt cggtgtcatc ttctgctcgt
      481 tctctgaggc cgtgaagaac catcccgacc tcgtgaagaa gtacctcggc tccgtcgtgc
      541 ctcacaccga caacttctat gcggccctca acgccgccgt cttcagcgac ggttccttcg
      601 tgtatgtgcc gaagggcgtc cgctgcccca tggagctctc cacctacttc cgcatcaacg
      661 cagccgagac cggtcagttc gagcgcacgc tgatcgtggc cgaagagggc gcctacgtga
      721 gctatctgga aggctgcacg gcgcccatgc gcgacgagaa tcagctccac gccgcagtgg
      781 tcgaactggt ggccctcgac aacgcccaaa ttcgctattc caccgtccag aactggtatc
      841 ccggcgacaa gaacggtaag ggcgggatct acaacttcgt caccaagcgc ggcaagtgtc
      901 tcggccgcaa ttccaagatt tcctggaccc aggtcgagac cggttcagct atcacctgga
      961 aatatccctc ctgcatcctg cagggcgaca actccgtcgg cgagttctat tccgtcgcgc
     1021 tcacgaacaa ctatcagcag gctgacaccg gcaccaagat gatccacctt ggcaaaaaca
     1081 ccaggagcac gatcgtatcg aagggcatca gcgccgggca cggacagaac acctaccgcg
     1141 gcatggtgaa gatcatgaag ggcgccgaag gcgcgcgcaa ttacacgcag tgcgattcgc
     1201 tgctcattgg cgacaagtgc ggagcgcaca ccttccccta catcgaggtg aaaaactcct
     1261 cggcgcggat ggagcacgaa gcggcaacgt ccaagatcgg tgaagaccag ctcttctacc
     1321 tgcagcagcg cggcctcaag accgaagacg ctgtctcgat gatcgtcaac ggcttctgta
     1381 agcaggtctt caaagagctg ccaatggaat tcgcagtcga ggcccagaag ctgctggccg
     1441 tcagcctcga aggcagcgtc ggatagtagg tttgtgcacg attcatcgcg tgccccatat
     1501 ctccctcctt ttttgcagat gtgggacggc agaatgcagg tgaatgcaac agcgcttttg
     1561 taacagggca cgacttcagt cgtgccgaag cagggattca aactcgcggc ggcttcagcg
     1621 ccgcgaaaac ggtagaagcc ccgggcttta gcccggggac aaaaggccgg aaatatcagg
     1681 ggctttagcc ccggcaatga cgagggaatc aatgagtttg ttggaaatca aaaatctgca
     1741 cgtgaacgtg gacgggaacg agatcctcaa aggaatcgat ctcaccctga atgccggcga
     1801 ggtccactcc atcatgggtc caaacggctc gggtaagagc accctggcgc aggtgctggc
     1861 gcgccgtgac gcgtacaacg tcacccaggg ctctatcacc ttcgacggca aagacctgct
     1921 ggagatgaag cctgaggaag ccgcctgcga gggagtcttt ctggccttcc aataccccgt
     1981 cgaaatcccc ggcatcggca acacctactt cctccgctca gctctgaacg cgcagcgcaa
     2041 atatcgcggc gaagacgaaa tggacgccat ggatttcctc cccatgttcc gcgaaaagct
     2101 caagctgctc gaaatggacg acaagctgat gaaccgctcg ctcaacgagg gcttctccgg
     2161 cggcgagaaa aagcgcaacg agattctgca gatggccgtg ctcgagccga agctcgcgat
     2221 tctcgatgaa accgactccg gcctcgacat cgacgccctg cgtattgtcg ccaatggcgt
     2281 caattcgctg cgcagccccg atcgcgccat gctcgtcgtt acccactacc agcgtctgct
     2341 caactacatc gtgcccgact tcgtccacgt gctggtcgat ggccgcatcg tccactcggg
     2401 cggcaaggaa ctggcgctcg aactcgaaga gaagggttac ggctggacgg agacacccca
     2461 gcccgtcgga gcgcgttcgt gagggagaca gccgtggccg tgtcgcatat cgatagctgg
     2521 ctcgagaact tcaccgattt cgagaagaaa gccgcgggcc gcactctgcc ctggctgcac
     2581 cggctccgtg aagacgcctt cgcgcgcttc tgcgaagtcg gttttccgac cacgaaggat
     2641 gaggactggc gcttcaccaa tgtctccggc atcgcaaaaa ccgtgtttcg tccggcatcg
     2701 actgcaaagc tcgcctccgg cgactccgcc gcatggcgcg tggacgatgt tgcagccacc
     2761 ctcgtcttcg tcaacggaca cttcgacgcg cagctttcga acatcggcaa tctcccgaaa
     2821 ggcgtcgccg tcgcaagcct gcgcgaacgg atcgcgtcgc atccggagga agtacagcag
     2881 cacctcggcc gctatgccga cacccagcgg gatgcgttcg tcgcactcaa taccgccttc
     2941 atcggcgatg gcgcgtatgt gcacgtcgct cgtggcgtcg tcctcgaagc gcccatctac
     3001 ctcctctacc tctccaccaa agaaaacgcc ccaaccatga cccatccgcg caacctgatc
     3061 gtcgctgaag accaggccca ggtcgcggtg attgaggatt acgcatcggt cggcggcgac
     3121 tccgccgtgc tctcaaacgc cgtaaccgaa ctgatcgccg gcgaaagcac agtggcgcag
     3181 cattacctca tcgagcgcga gcacctgaag gcctggaaca tctccaccct ccgcattcag
     3241 cagggacgca gcgccaatgt cgcctcccac tccttgctgt tgggcggcgg cctcgtgcgc
     3301 aacaatgtcc accccgtcct tgccggcgaa ggcggtgaat gcctcatcaa cggcctcttc
     3361 ctcggcacag gcgcgcagca cctcgacaat tacatgcacg tagagcatgc ggcgccccac
     3421 tgcggtagcc gccagttcta caacggcatc ctcgacgggc attcgcacgg cgtcttccac
     3481 ggccgcatcg tcgtccacaa ggacgcgcag aagaccgacg cgaagcagac caatcgcaac
     3541 ctgctcctga gcgacgatgc ccagatcgac accaaacctc agcttgagat ctacgccgac
     3601 gacgtcaagt gcacccacgg cgccaccatc ggccagatcg aagagaacgc cctgttctac
     3661 ctgcgctcgc gcggcatcag tgagcaggaa gcaaagaagc ttctcctgat ggcctttgcc
     3721 gaggagtgcg tcgagcgtat gcagtccagt gtcgcccgcg agcatatcga gcgcctcgtc
     3781 aacgagcacc tcttgttcac tgaaggcgtt gtgccgccgc cagccgcaga gcgcgtgagc
     3841 gaaagaaccg tggaggaagt gggatgagcg ccgcaaccgc tgttccgatt cgagaaaaag
     3901 ccgcgtcctt cgatgtggac cgcatccgca gggactttcc catcctgcac accaaagtcc
     3961 acggccatcc tctggtgtat ctcgacaacg ctgccacgtc gcagaagccg caggccgtaa
     4021 tcgacgcttt ggtgcgctac taccagggcg agaacgcaaa catccaccgc ggtgtgcact
     4081 acctctccga actggcgacg gcggagttcg agaaggcgcg cgagaccgtg cgagccttca
     4141 tcaacgcagc ccggtccagc gagatcatct tcacccgcgg caccaccgaa tcgctcaacc
     4201 tcgtcgccca aacgtacggc aagtcgaacc ttcgcgctgg cgacgaagtg ctgatcaccg
     4261 ccatggagca ccactccaac atcgttccgt ggcagatgct gtgcgaagaa aaaggcgcaa
     4321 agctccaggt ggcccccatc aacgatcagg gcgagctgct cttcgccgag tacgaaaagc
     4381 gaatcggccc gaagacaaag attgtcgcgg tcggccacgt atcgaacgct ctcggaacca
     4441 tcaatcccct ccggcagatg attgcggcgg cccatgccca gggcgccatc gtcgtcgtcg
     4501 atggcgccca ggccgttcca cacctcaggg tcgacgtgca ggcgctcgat gctgacttct
     4561 atgccttctc cggccacaag atgtacggcc ccaccggcat cggcgtcctc tacggcaagc
     4621 aggcactgct cgaagccatg cctccgtacc agggcggggg cgacatgatc agctccgtca
     4681 ccttcgagaa gacgacgtac aacaaggtgc cccacaagtt cgaagcgggc actccggata
     4741 tggccggcgt cgccggcctc atggcggcca ttgagtacat gaacgccatc ggcatcgaca
     4801 ggatcgctgc acacgagcat gatctgctcg tgtacgcgac cgaagtcatc cagtccgtgc
     4861 caggcgtgcg cctcatcggc accgcagcgg aaaaggcggg cgtgttgtcc ttcgtgctcg
     4921 acgaggtcca tccccacgac atcggcacaa tcctcgatca gcagggcata gcagtgcgca
     4981 caggacacca ttgcgcgcag ccggtcatgg agcgcttctc gatccccgca accgtgcgcg
     5041 cctccttcgc cgtctataac accaaagccg aagtcgacgc gctggcacgc ggtatccgca
     5101 aagtgcgcga ggtgtttgcc tgatgccgga gctgagagat ctctaccagg aagtgattct
     5161 cgagcacagc aaggcgccga gaaactacaa ggcgctcgag agcgcgaacc acaaagccga
     5221 gggctacaac ccgctctgcg gcgaccgctt cacggtgtat ctccagatgg acggcgacac
     5281 cattcgcgag attggcttcc agggcgccgg ttgcgccatc
//
LOCUS       DASZYN010000282         6404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_140266, whole genome shotgun sequence.
ACCESSION   DASZYN010000282 DASZYN010000000
VERSION     DASZYN010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140266"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VHE33_07230"
     CDS             <1..272
                     /locus_tag="VHE33_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97285.1"
                     /translation="RGDLHSDLLKIGHHGSRTSTTPSFLAAVAPSWAAVSVGRRNFYG
                     HPRPEVLDELQISHVRTFRTDTLGLSTFYFDGNSVHAAVWAAESR"
     gene            complement(311..1585)
                     /locus_tag="VHE33_07235"
     CDS             complement(311..1585)
                     /locus_tag="VHE33_07235"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT97286.1"
                     /translation="MRIVFSTYGTFGDVNPLIALSLELKRRGHKPVLAIPEMFRQKVE
                     PLGIGFVPVRPDQDPNDTRLIAMIYDIKHGTETGLRKFLFPSLRESYQDLMAAVQADG
                     GADLLVTGELAYAGPIVAEKSGIPWASYVLAPFSFFSGYDPPVLPPYPVLAKMQDAVP
                     PLGHVVARYARFVTRKWPEPVYELRRELGMAQGRNPIFDAKHSESLVLALYSRVMGNP
                     QPDWPRAVKITGFAFYDGAGAEGLLPPELVSFLDDGPAPLVFTLGSAAVMHAGDFYEQ
                     SAEAAEILGQRAVLLIGADERNLPKRKLPASICVARYAPYSKIFPRASAIVHQAGIGT
                     TAQALRAGRPMLVMPYSHDQPDNARRVRKLGVAEVLDRKEYKAKTASRLIAQLLSDNG
                     HGVRAAEVAKKVAAEDGTVTACDALEAMGQRG"
     gene            complement(1630..3180)
                     /locus_tag="VHE33_07240"
     CDS             complement(1630..3180)
                     /locus_tag="VHE33_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97287.1"
                     /translation="MFRTRRVCALAVGGCALVAGSCSIAAQAVSPAESRTARAFDAAR
                     REGPPALRAFLYRMPKGADLHVHLSGAVYAETFIREAGEDGICVSARLLELDDQHHAP
                     DCPAGETAAKDLPENQHLYDEIVDAISMRTFVPKTGESGHDHFFDTFDKFGDDKRFTG
                     EWIDEVASRAAAQNEQYLELMQTPGFKAAKALGDNFGWNGDPAELREKLLAEPSFRAN
                     VESDRAELAAAREQRNQIEHCGQADAAPACKVTVRFLFQVLRGHPPQQVFAQTLLGFE
                     VASADSDVVGINFVMPEDGYISMRDYALQMRMLDYLHAQYPKVHITLHAGELAEGMVP
                     PDGLKFHIRMAVEQGHAERIGHGVDVMYEDRPWELMKEMAERHVMVEVNLTSNDVILN
                     VKGTDHPFMLYRKAGVPVALSTDDEGVSRIDLTHEYVRAADTYPLSYHDLKLMVRTSI
                     EHSFLPGASLWERVTPEKLETPVAACRGQLGNESPHGACAALVHGSEKARQEWELERR
                     FHAFEAAF"
     gene            complement(3301..3525)
                     /locus_tag="VHE33_07245"
     CDS             complement(3301..3525)
                     /locus_tag="VHE33_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97288.1"
                     /translation="MDRSAQMKRMMRDYDRDHAINSTGFGHRSDSAENRFDEYTWLTM
                     QTLRAILTDSHLWVPLLVLVAGIALLMRLI"
     gene            3537..4853
                     /locus_tag="VHE33_07250"
     CDS             3537..4853
                     /locus_tag="VHE33_07250"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HVT97289.1"
                     /translation="MTSHFDTKHSPATDLPSALNGHFALGIVCALAAGAWLGAAEAPA
                     RLAASGFGPFAISFCMVGGVFLARWTLPIAAKGTRSVARDLLQRPHLMIWAVVAGGLW
                     AVANTLTIFAIRDVGLSIAFPLWNTNSLIGIFWGWALFHELDGASTRSWLRVAGGAAA
                     IVLGSVLLSALSVHTMAATPGRAAAGIAAALGAGVLWGTMYVPYRKAYLSGINPLSFV
                     TIFTFGELGTMFFLAIVLPGGLSGLGHQLASMRSSIFWLLLGGFCWVIGDLFQQYATK
                     YVGISRAIPLSNTNQLWGFAWGVLVFGELAGTHGSVRLMALGASAITIFGAMLIAGTT
                     VSSAESASTVRAIRRECDRYDMDMAATLQTQTVQVTNQPAAAGRRSWFDYLILLAAVA
                     IFVWFGHFVRMPQVALSIPAVIVLTAILFAVLCGAGWLLWKKTRFD"
     gene            4926..5894
                     /locus_tag="VHE33_07255"
     CDS             4926..5894
                     /locus_tag="VHE33_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HVT97290.1"
                     /translation="MPIDIVGVGLNATDTLIPVSHYPASGSKVEIRAASVLPGGQVAT
                     AMVACQLWGLRARYVGKLGDDQAAALHSEEFRKSGVETRLITAPACSSQQAFILVEQS
                     GERTVLWKRDDRLTLQPSDLCREWITEARALHVDGHDTAAAITAATWAREAGIPVVAD
                     LDDLYPGVEALIEKIDYLIVSRDVPSRLTGKADLFEALAAVQKKFGCLLTAATLGHEG
                     VVACMGAAFCHRPAYHVEVVDTTGAGDIFHAGFIYGLLREWTLSQTLDFACAAAALNC
                     TGTGARGGIQPVSVIENLIASDARHPTEFTALAAHRNTACNVELKP"
BASE COUNT         1216 a         2144 c         1709 g         1335 t
ORIGIN      
        1 tccgtggaga tctccattcc gatctcctca aaatcggcca ccacggcagc cggacttcga
       61 ctacccccag ttttctcgcc gctgttgcgc cttcctgggc agccgtctcc gttggccggc
      121 gcaacttcta tggtcatcct cggcccgaag tcctcgacga gctccagatt tcccacgtcc
      181 gcacgttccg cacggatacc ttaggcctgt cgacgttcta tttcgatggg aattcggttc
      241 acgcagcagt ctgggccgca gagtctcgct aaggccatcg ggtatccacc atcgcagccc
      301 aacgagatcc tcagcctcgc tgtcccatcg cttccagcgc atcgcacgcc gtaaccgtcc
      361 cgtcttccgc agccaccttc tttgccacct cggccgcgcg aacgccgtga ccgttgtccg
      421 aaagcagttg cgcgatcagg cgagatgccg tttttgcctt gtactctttt cgatccagca
      481 cctcggcaac ccccagtttg cgtacacgcc gggcattgtc cggctgatcg tggctgtagg
      541 gcatcaccag catcggacgc ccggcacgca gcgcctgcgc tgtcgttccg attcccgcct
      601 gatggacaat cgccgacgct cgcggaaaaa tctttgagta gggtgcgtac cgcgccacgc
      661 agattgatgc cggcaacttg cgcttcggca gattccgttc gtccgcccca atcagcagca
      721 cagcacgctg tcccagaatc tcggccgcct cggcgctctg ctcatagaag tcgccggcgt
      781 gcattaccgc tgccgaaccc agcgtgaaca ccagcggcgc cggcccgtca tcaaggaacg
      841 acaccagttc cggaggcaat aagccctccg ctccggcacc gtcataaaag gcgaatcccg
      901 taatcttcac cgcccgcggc cagtctggct gcggattacc catgacgcgc gaatacaggg
      961 ccagaaccag actctcagaa tgctttgcat cgaaaatcgg attccttccc tgcgccattc
     1021 ccagctcacg gcgcagttcg tagaccggct ccggccattt ccgtgttaca aaccgggcat
     1081 atcgcgcgac aacatggccg agcggcggga cagcatcctg catctttgcc agcaccggat
     1141 acggcggaag caccggcgga tcgtatccgg aaaagaaaga aaacggcgcc agcacgtacg
     1201 aggcccacgg aatgccgctc ttttccgcga caatcggtcc cgcgtaggcg agctcacccg
     1261 tcaccagcag gtcggcaccg ccgtccgcct gcactgcagc catcaggtcc tggtagcttt
     1321 cgcgcaagga aggaaaaagg aatttgcgca gtccagtctc cgtcccgtgc ttaatgtcgt
     1381 agatcatcgc aatgagccgc gtatcattcg gatcctgatc aggacgcacc ggaacaaacc
     1441 cgatgcccag cggttcgact ttctgacgaa acatctccgg aatcgccagc actggtttgt
     1501 gcccgcgtcg cttcagttcc agcgagagag caatcaacgg attcacatcg ccgaaggtcc
     1561 cgtaagtgga aaaaacgatt ctcatcgcgc gcaagcgtcc ggctcatgcc gaatctcgct
     1621 cagctctcct tagaaggcag cttcaaatgc atggaaccgc cgttccagct cccattcctg
     1681 ccgtgctttc tcgctcccat gcacgagtgc cgcgcatgcc ccgtgtggtg actcgttacc
     1741 taactgtccg cgacacgcgg caaccggcgt ctccagcttt tccggagtca ccctctccca
     1801 taagctcgct cccggcaaaa agctgtgctc gatcgaagtc cgcaccatca gcttcaaatc
     1861 gtgatagctc agcggatatg tatcagccgc gcgtacatat tcgtgagtca aatcgatccg
     1921 cgaaacaccc tcatcatcgg tcgacagcgc cactggcact ccggccttcc gatacagcat
     1981 gaagggatgg tccgtgcctt tcacgttcag gatcacgtcg ttgctggtca ggttaacctc
     2041 caccatcacg tgccgctccg ccatttcctt catcagctcc cagggccgat cctcgtacat
     2101 cacgtccacc ccgtgcccaa ttcgctccgc gtgcccctgc tccaccgcca tgcggatatg
     2161 aaacttcagc ccatcgggag gcaccatgcc ctcggccagt tcgcccgcat gcagcgtgat
     2221 atgaactttg ggatactgcg cgtgcaggta gtcgagcatg cgcatctgca atgcgtaatc
     2281 acgcatggaa atatacccgt cctctggcat aacaaagtta attcccacta catcggaatc
     2341 agcagaagca acttcgaagc caaggagcgt ttgcgcgaac acctgctggg gaggatgacc
     2401 tcgcagtacc tgaaaaagga agcgcacggt gactttgcac gccggagccg cgtcggcctg
     2461 tccgcaatgc tcgatttgat tccgttgctc gcgcgcggcc gccagttccg cacgatcgct
     2521 ttccacgttg gctcgaaacg atggctccgc cagcagtttc tcgcgtaatt ccgccggatc
     2581 gccattccat ccgaagttgt cccccagcgc cttcgcggcc ttgaatcccg gcgtctgcat
     2641 cagttccaga tactgttcgt tctgcgccgc cgcccgcgag gcgacctcat cgatccactc
     2701 cccggtaaac ctcttgtcgt cgccaaactt gtcgaaggta tcgaagaagt gatcgtggcc
     2761 cgactcgcct gtcttcggca cgaaggttcg catcgaaatc gcatcgacga tctcgtcgta
     2821 caagtgctga ttctccggca ggtccttcgc cgccgtttca ccggcaggac aatccggtgc
     2881 gtgatgctgg tcatccagct ccagcaacct ggcgctgaca caaatcccat cctcgccggc
     2941 ttcacggatg aaggtctctg cataaaccgc cccactcagg tgaacgtgca ggtccgcacc
     3001 ctttggcatc cggtagagaa aggcgcgcag agccggaggc ccctccctgc gggccgcatc
     3061 gaacgcacgc gcagtccgtg attcagccgg cgaaacggcc tgcgcggcga tgctgcaact
     3121 gcccgcaacc aaagcacaac cgcccacggc aagggcgcag acacggcgag tccggaacat
     3181 gagccttgag tttcctttga gaatggagat gcagtgctcc aagtttatcg cgggcatgag
     3241 cggagcccga tcacccattg cccacacacg cgggccacag gatccggcga aaccgacggc
     3301 ttaaatcagc cgcatcaaca aagcaatgcc cgccaccagt accagtagcg gaacccatag
     3361 atggctatcg gtcaggatcg cgcgcaacgt ttgcatcgtc agccaagtat actcatcgaa
     3421 tctgttttct gctgagtcgc tccgatgacc gaaacccgtc gaattgatcg catgatcgcg
     3481 atcgtaatct cgcatcatac gtttcatctg ggcgctgcgg tccatcggag catcttttga
     3541 cgtcgcattt cgacacaaag cattcacccg caaccgatct tccttccgcc ctcaatgggc
     3601 acttcgcgct gggcatcgtc tgcgcgctcg cggccggtgc atggctgggc gcggccgaag
     3661 ctcccgcccg cctggcggcc tccgggttcg gaccgtttgc catctccttc tgcatggtgg
     3721 ggggcgtctt tctcgcacgc tggaccttgc ctatcgctgc caaaggcacg cgttcagtgg
     3781 cccgcgattt gctgcagcga cctcacctca tgatctgggc tgtcgtcgcc ggcggcctct
     3841 gggctgtcgc caataccctt acgatctttg ccattcggga cgtcggactc tccatcgcat
     3901 ttccactgtg gaacaccaac tcgctcattg gaattttctg gggctgggcg ttgtttcacg
     3961 aactcgatgg tgcctccact cgcagctggc ttcgcgtcgc cggcggagcg gccgctatcg
     4021 tcctcggatc cgtgcttctg tccgcgctct ctgtgcatac catggctgcc acgccgggtc
     4081 gcgctgccgc cgggattgcc gccgcccttg gagcaggcgt gctctggggc accatgtacg
     4141 tcccttaccg caaggcgtac ctcagcggca tcaatccact ctccttcgtg acgatcttta
     4201 ccttcggcga actcggcacc atgttcttcc tggccattgt cctgcccggc gggctctccg
     4261 ggctcggcca ccaactcgca tccatgcgtt cctcgatctt ttggctgctg cttggaggat
     4321 tctgctgggt catcggcgac ctgttccagc aatacgcgac gaaatacgtg gggattagcc
     4381 gcgccattcc actttccaat accaaccagc tatggggatt cgcatggggc gttctcgttt
     4441 tcggcgaact cgcgggtacg cacggttcgg tgcgcctgat ggcccttggc gcctcggcga
     4501 tcaccatttt tggagcgatg ctcatcgccg gcaccaccgt ttcatccgcc gaatctgcgt
     4561 ccaccgtccg cgccattcgt cgtgaatgcg atcgttacga tatggatatg gcggcaacgc
     4621 tgcaaacgca gacggtgcag gtgacgaatc aacccgccgc tgcaggtcgt cgctcctggt
     4681 tcgactacct gattctcctc gcagcggtcg ccatctttgt ctggttcggg catttcgtgc
     4741 gaatgcccca ggtcgccctc agcattcctg cggtcatcgt gctcaccgcc attctctttg
     4801 ctgtcttgtg tggtgcaggc tggttgctct ggaagaaaac ccgcttcgac taaagctccg
     4861 cgcgcaggct ttggatcagc ctgagcagtc cgttaacatg gtgattcaca ctctggaaac
     4921 cgcctgtgcc aattgacatt gtgggcgtcg gtctgaacgc caccgatacc ctgattccgg
     4981 tctcgcatta tcccgcctct ggttccaaag tcgaaatccg tgccgcgtcg gtgttgccgg
     5041 gcggccaggt cgccaccgcg atggtcgcct gccagctttg ggggcttcgc gccagatacg
     5101 tcggcaaact tggcgacgat caggcggcag cgctccattc tgaagaattt cgcaagtctg
     5161 gcgtcgaaac ccgcctcatc acggctccag cctgttcaag ccagcaggcc ttcatcctcg
     5221 tggaacaatc cggcgagcgt acggtcttgt ggaagcgcga cgatcgcctt acccttcaac
     5281 ccagcgacct gtgccgggag tggatcaccg aagcgcgcgc gcttcatgtc gatggccacg
     5341 acaccgccgc ggcgatcacg gcagcaacat gggctcgcga ggccggaatt cccgtcgtcg
     5401 ccgacctcga tgatctctat ccaggtgttg aagcactcat cgaaaagatt gactacctca
     5461 tcgtcagccg cgacgttccc tcccgcctca ccggtaaggc ggatctcttt gaagcgcttg
     5521 ccgcagtgca gaagaaattt ggctgccttc tcaccgccgc gactctcggc catgaaggcg
     5581 ttgtagcctg catgggcgct gctttttgtc accggcctgc atatcatgtt gaggtcgtcg
     5641 acaccacagg cgcaggcgac atttttcacg ccggcttcat ttacggtttg ctgcgcgaat
     5701 ggactctctc gcaaacattg gattttgcct gcgccgccgc tgccctcaac tgcaccggaa
     5761 ccggcgcgcg aggtgggatc cagcccgtct ccgtcattga aaacctcatc gcgtctgacg
     5821 cgcgtcatcc gaccgaattc accgctctcg ccgcccatcg caacacagcc tgcaacgtcg
     5881 aactgaagcc gtagttccgt tcgcctttgc cgcgagcctc cagtctgaac gcagtcaccg
     5941 cctgactgat ttcgtcggag cttgagcagc gggattcggg cagcacttcg gcgtggcttg
     6001 cttctctagt ggaagtaagc cctcaccagt gggaaaatat tgaactcact cctgtcgtat
     6061 acggcacacg caaccagcac ggaggtgcgc cgacgaacgg ctacaaatcc cagcccccac
     6121 cgcggtgaat acgaggcggc aacacgaatt acaacagttc acgctggagc accgcctgcg
     6181 aaattcacag cgggaaagtt atttctcaca gaatgacctg cgcgtgcact tcggtctggt
     6241 cagagcgagg acgatcgata actcgagatt cactggccct caggcaaggt cgagcaactc
     6301 accagccttc cggcggatca ctacgatagc gtagtggaag gcaaaggcat tgtggactct
     6361 acccgcgccc ggccctcacc catcgagcgc gcagccgcgg cgca
//
LOCUS       DASZYN010000283         8288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_846331, whole genome shotgun sequence.
ACCESSION   DASZYN010000283 DASZYN010000000
VERSION     DASZYN010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8288
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846331"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..1851
                     /locus_tag="VHE33_07260"
     CDS             307..1851
                     /locus_tag="VHE33_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HVT97291.1"
                     /translation="MTVAVHPSAKTKRRIHVTEAVTAALIALAMIAVHLMFFFWPFRY
                     REVHPLLEEVFESKVTVSGYHRTYFPNPGFVAEGVTFYRHGDTRIPPLATVRRMTVKG
                     QWGMLLFHPHLLYEIRLQDLHVQIPPAGTIARGKDFDNGVVDTTGSKLKIETICADGT
                     VLDFLRNGYGPIRFNFPALQIHDLTAGRPMDFSLRVITPGPEGTLTASGKIGPFRTSS
                     YATTPLSGTYEIKSADLSRLEGLAGHVRGGGRFSGKFTRIEVDGTAAIPDFRAADAHT
                     VGLATEYRIAVNGANSDVQIERAQVKTGSSVITASGTVAGSPRRVAVAFATQAADVQD
                     LLRMVETAEPEVAGKVNLDANAEFTEGPQPFLEKLRLKGDVAVEGLRFVDAKKQQTMD
                     AFSERVRKDAPKDANGGDPPQVTLAARSETRFDHGMAYFPQIRAVLPGATARLHGTFG
                     LLDSRVHLKGTVALDKSISHATTGWKSVLLKPLSPFFKKKNVGALVPIAVTGTAQSPK
                     VGADIF"
     gene            2125..4548
                     /locus_tag="VHE33_07265"
     CDS             2125..4548
                     /locus_tag="VHE33_07265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT97292.1"
                     /translation="METLGRNLRFSLRMLGRNPGLTLTILLTLALGIGANTAIFTVDY
                     ATLLAPLPYPEPDQLVEVWSKIQTFHNGISAGDYTDWKNQATVFQDLCAWSGASFNVA
                     TKDQPEYIEARTVSVGFLRMIGEPLLMGRFFLPEEGVDGKDHVTVLTHKFWVKLGADP
                     HIVGTTIRLNGEPYTVVGVQKPGVWDKGQAQLTVPLVFKPEQLNHDFHWLLVMGRLKP
                     GVTIQQAQQNMDSVTKHIAEQYPKSNKGWGSYVEPLKNDFMPKERIQMLWLLLGAVGF
                     VLLIACVNVANLLLARGMTRQKEMAVRGALGAGRKTIFGQLLTESLLLALAGGVLGVG
                     VGYGMLQGLIAVMPQDTLPYEADLRLNVPILLFTLLTTTLAGVLAGSMPAWYASRIDP
                     AETLKEGGRTGTSTGKHRLRRVLVVGEFTLALVLLAGAGLAIHSFWNLTQVDLGVKTD
                     HVLTFYLPVPDSRSKDPAQISAYYRDILSRIDGVPGVQHASVQTGVPLYGAGFGMPFT
                     IVGGPTYADPSQRPGAGFGMVTGDYLSTFGIRMVKGRAISDQDTASSVKVMMVNESFV
                     KSYLKGKDPLRERISVEQIIPGVQKLGPPQDWQIVGVYHDVHRADRRDHNEEMLIPFW
                     QIPWPQAGIGVRTAQDPLSMQRSIANAVHSVDPDIALAQPQTLEQIRSDTMSDQRFTL
                     ILFGTFAVVALFLAVLGIYGVMAFSVVQRSHELALRMALGASRRRLVTMVVREGVILA
                     SVGLGLGLVGAYFVGRAMQSMLFDVHPLDLVAFVSVGLVLMASAVLACYWPARRAASV
                     HPMQVLRME"
     gene            4731..5012
                     /locus_tag="VHE33_07270"
     CDS             4731..5012
                     /locus_tag="VHE33_07270"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HVT97293.1"
                     /translation="MPKFVIEREIPGAGKLTKDQLQAISQTSCGVLRKMGPEIQWVES
                     FVTEDKIYCIYNAPDAAMVRKHAEMGGFPANSVARVSTIIDPTTSEQSS"
     gene            5166..7103
                     /locus_tag="VHE33_07275"
     CDS             5166..7103
                     /locus_tag="VHE33_07275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HVT97294.1"
                     /translation="MKRFPVAQGLPLARTQERLLPSGLVWAAVAVLLVVQAVLVLACV
                     HRESLTFDEDDHMFAGYMMWKAGDYGLNPEHPPLVKLLATVPVLGEKMWIPPDQGREF
                     KKEAYLDGRDWLARNDGASQRLVFRMRMITGLLAWGLSLVVFFATREWFGDGAALVAM
                     TLAVFDPNIMAHSALVTTDVGVTLGFLATIWTFYRFMRQPTVLRLLVAGVVAGLLAAT
                     KHSGILVVPMLAPAMIWEVLAAQPGERARQAMRIVSGMVAIVVVAAVVLWAFYGFRYG
                     SRPPGLLSTSLAEYVQPLSGFDQAVVLTVGRLHLLPESYLIGLADVKRMAQFYPTFAL
                     GKVYTHGVWWYFPLAILIKTTLGLLALNAVALWAAVTGKIGKAREVFYVLSPGAVYLA
                     IAMASKMDIGARHILPLYALAAIFAGAGAAALARGSKAWAGVGIALLAVHVASSMVNY
                     PQQMAYANEAWGGAKNTHNLLSDANVDWAQQLYQVKDWQDRHPGEACWFAYFARPEVD
                     PAVYGITCHALPNADTGWLGEGQEVIPPVVHGAVLISAGDLSGCEFPDERMNPYREFM
                     HVLPSEVIEDAVFVYRGEGGQGVHVEQAAALSRAQRARVLLEQHQAAQALALAREAVA
                     IAPEEPMAGRILQAAEKALGK"
     gene            7327..>8288
                     /locus_tag="VHE33_07280"
     CDS             7327..>8288
                     /locus_tag="VHE33_07280"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HVT97295.1"
                     /translation="MASRPTQSGMGRYGRKGPSFPESVARFWNRVTEGMQVGELWKQF
                     RADARTSYRLYSQEVDTAASAGTPRGRHFVQVAKQFFWAFLEKLTPARRVLLLVAIVL
                     TVMPGSQWHWHWGRSDVGFSTPDFRLYGSLLMLALLLMEVGDRVVMKRDLQIAKEIQA
                     WLLPAEPPSVPGLEIAFATKPANTVAGDYYDVFARPGSDGRTWLLAVADVAGKSVPAA
                     MLMATFQASLKTLSHTPSALTELVGRINRYACTNSQNGRRFTTAFIAEYEPATRRITY
                     VNAGHNNPILLRRSGAVERLDVGGMPLGVIDGAPYASGEVTLEVG"
BASE COUNT         1513 a         2001 c         3168 g         1606 t
ORIGIN      
        1 gagacaggca gggagacgtc gcatggtcat gtttagcgta tagggtaaag ggtttgggtg
       61 aaaagctggc taaggggcgt tcagctgatg aagggccagt gaaagcgagt caaaaacgag
      121 ccaacagctt gtgaaatccg agcgaacagc gagggaactg gaagcaaacc gcgagcgagg
      181 cgagcgagct gcgagtgaaa atgtgaattc ggttgaggct tgcgggttgg atgttccaca
      241 gagacgaggg acaaccacgg cgcacgtggg ggtcatctgg actgatgggc ccgggaattc
      301 gaggcgatga ctgtagccgt ccacccttcc gcaaaaacca aacgccggat tcacgtgaca
      361 gaagcggtca ccgctgcttt gatcgcgctg gcgatgatcg cggtgcatct gatgttcttc
      421 ttctggccgt tccggtatcg ggaggtgcat ccgctgctgg aagaggtgtt cgagagcaag
      481 gtaacggtga gcggatatca ccggacgtac ttcccgaacc ctggatttgt cgcagaggga
      541 gttacatttt accggcacgg ggatacgcgc attcctccgc tggcgaccgt gcggcggatg
      601 accgtgaagg gccagtgggg aatgctgctg ttccatccgc atctgctcta tgagatccgg
      661 ctccaggatc tgcatgtgca gattcctccg gctgggacga tcgcgcgggg aaaagatttc
      721 gacaacggcg tggtcgacac caccggttcg aagctgaaga tcgagacgat ttgcgcggat
      781 gggacggtgc tggattttct gcgcaatggg tatggcccga tccgattcaa cttcccggcg
      841 ctgcagatcc atgatctgac ggcggggcgc ccgatggatt tttcgctgcg ggtgatcacg
      901 cctggaccgg aggggacgtt gacggcaagc gggaagatcg gaccgtttcg gacaagcagt
      961 tatgcaacga cgccgctgtc ggggacgtac gagatcaaga gcgccgatct gagccggttg
     1021 gagggacttg cggggcatgt acgaggcggg ggtaggtttt cggggaagtt cacccggatc
     1081 gaggtggatg gcacggcggc gattcccgat ttccgcgcgg cggacgcgca cacggtaggg
     1141 ctggcgacgg agtaccggat cgcagtgaac ggcgcgaaca gcgatgtgca gatcgagcgc
     1201 gcgcaggtga agacggggag cagcgtgatc accgcgagcg gaacggtggc cggatcgccg
     1261 cgtcgggtgg cggtggcctt tgcgacgcag gccgccgacg tgcaggatct gctgcgcatg
     1321 gtggagacgg cagagccgga ggtggcgggg aaggtgaacc tggacgccaa tgcggaattc
     1381 acggaggggc cgcagccatt tttggagaag ctgcgattga agggtgatgt tgcggtggag
     1441 ggattgcggt ttgtggatgc gaagaagcag cagacgatgg atgcattcag cgaacgggtg
     1501 cggaaagatg caccgaaaga tgcgaacggc ggtgatccgc cgcaggtgac cctggccgca
     1561 cgttcggaga cgcgtttcga ccatgggatg gcgtatttcc cgcagattcg tgccgtgttg
     1621 ccaggggcga cggcacgttt gcacgggacg tttgggctgc tcgattccag ggttcacttg
     1681 aagggaacgg ttgcgctgga caagagcatc tcgcatgcga cgacggggtg gaagtcggtg
     1741 ctgctgaagc cgctgagccc gttcttcaaa aagaagaacg tgggggcgct ggtgccgatc
     1801 gcggtgacgg gaacagcgca gagcccgaag gtgggggcgg atatcttttg agctggtgaa
     1861 gcggtcagcc agggtccggt tagctggtaa agcggttagc gaagattcgg tcagcgaagt
     1921 cccatcagct ggttggcgtg cgatgcgtcg cggtcgccac ttcgtcacat ttcaaaactg
     1981 aaggaacctt atttcgttac gctgcgtaac agacggcggt gggggctgcg gtgtgtcctg
     2041 ctgagcgaag aagagactgc acacgtctaa gctgcacggc ccactgagca ttgcccggtt
     2101 gcattacgca ctgagggggt gtgtatggag acgctggggc ggaatcttcg attcagtttg
     2161 cggatgctgg gacggaatcc cggcctgacg ctcaccattc ttctgacgct ggcgctgggg
     2221 attggcgcga atacggcgat cttcaccgtg gattacgcaa cgctgctggc gccgctgccc
     2281 tatccggagc cggaccagct ggtggaagtg tggtcgaaga ttcagacctt ccataacggg
     2341 atctcggcgg gagattacac ggactggaag aaccaggcga ccgtattcca ggatctgtgc
     2401 gcatggagcg gcgcgagctt taacgtggcc acgaaggatc agccggaata catcgaggcg
     2461 cggacggtat cggtgggatt cctgcgcatg atcggggagc cgctgttgat gggccggttc
     2521 ttcctgccgg aagagggtgt ggacgggaag gatcacgtca cggttctgac gcataagttc
     2581 tgggtgaagc tgggcgcgga tccgcacatc gtggggacga cgatccggct gaacggagag
     2641 ccgtacacgg tggtgggtgt gcagaaaccg ggagtatggg acaaggggca ggcccagctg
     2701 acggttccgc ttgtgtttaa gcccgagcag ctgaatcacg attttcactg gctgctggtg
     2761 atgggtcggc tgaagcccgg cgtgacgatc cagcaggcgc agcagaacat ggactcggtg
     2821 acgaagcata tagccgagca gtatccaaag agcaataagg gatggggttc ttatgtggag
     2881 ccgctgaaga acgacttcat gccgaaggag cggatccaga tgctgtggct gctgctgggg
     2941 gcggtgggat tcgttctcct gattgcctgc gtgaacgttg cgaatctgct gctggcgcgg
     3001 ggcatgacgc ggcaaaaaga gatggcggtg cgcggcgcgc tgggggcggg gcggaagacc
     3061 atctttggcc agttgctaac cgaaagcctg ctgctggcgc tggcgggcgg cgtgctcggc
     3121 gttggggtgg gttacggcat gctgcagggg ctgatcgcgg tgatgccgca ggacacgctg
     3181 ccgtatgaag ccgatctgcg gctgaacgtt ccaatactgc tgttcacgct gctgacgacg
     3241 acgctggcgg gagtgctggc gggatcgatg ccggcgtggt acgcgtcgcg gatcgatccg
     3301 gcggagacgc tgaaggaagg cggacggacg ggcaccagca cagggaagca tcggctgcgg
     3361 agagtgctgg tggtgggtga gttcacgctg gcgctggtgc tgctggcggg agccggactg
     3421 gcaatccaca gcttctggaa cctgacacag gtggacctgg gggtgaagac ggatcacgtg
     3481 ctgacgttct atctgccggt cccggattcg cgctcgaagg atccggcgca aattagtgcc
     3541 tactaccgcg acatattgtc gcggatcgat ggggtgccgg gggtgcagca tgcgtcggtt
     3601 cagacaggag tgccgttgta tggggccgga ttcgggatgc cgttcacgat tgtgggcggg
     3661 ccaacgtacg ccgatccgtc gcagcggccg ggcgccggat tcgggatggt gacgggagat
     3721 tatctgtcga cgttcgggat caggatggtg aaggggcgcg ccatcagcga tcaggacacg
     3781 gcgagcagcg tgaaggtgat gatggtgaac gagagcttcg tgaagtctta cctgaaggga
     3841 aaagatcccc tgcgggaacg gatttctgtc gagcagatta ttccgggtgt gcagaagctg
     3901 gggccgccgc aggactggca gatcgtgggg gtctatcacg atgtccatcg tgcggaccgg
     3961 cgcgatcaca acgaagagat gctgattccg ttctggcaga ttccgtggcc gcaggcgggg
     4021 attggcgtgc ggacagcgca ggatccgctg tcgatgcaga ggagcattgc gaatgcggtg
     4081 cactcggtgg atccggatat cgcgctggcg cagccgcaga cgctggagca gattcgcagc
     4141 gacaccatgt cggatcaaag attcacgctg atcctgtttg ggacgtttgc tgtggtggcg
     4201 ctgttcctgg cggtgctggg gatttacggc gtgatggcct tcagcgtggt gcagcgttcg
     4261 cacgagctgg cgctgcggat ggcgctgggc gcctcgcgca gacggctggt gacgatggtg
     4321 gtccgcgagg gcgtgattct ggcatcggtg gggcttggcc tgggcctggt cggcgcgtac
     4381 tttgtggggc gagcgatgca aagcatgctg ttcgacgtgc acccgctgga tctggtggcg
     4441 tttgtttcag tggggctggt actgatggct tcggcggtac tggcatgcta ctggccggcg
     4501 cggcgggcgg cgtcggttca tcccatgcag gtgctgagga tggagtgagg gcgagctaag
     4561 agccggttcg ctgaaatccg gttagcggaa aagcggatcg tgcgagcggt cctggtaatt
     4621 cttcgatagt gaaacgacag cgggcgcttg tacactctac ggggtcggcc ggagttgctc
     4681 ttctttttgc ggaaacggcg atgcagaccg tcaagtgtgg agatgagcct atgccgaagt
     4741 ttgtgatcga gcgggagatt ccgggcgcgg gaaagctaac gaaggaccag ttgcaggcaa
     4801 tttcgcagac ctcttgcggg gttctgcgca agatggggcc ggagatccag tgggtggaga
     4861 gcttcgtgac cgaggacaag atctactgca tctataacgc gccggatgcg gcgatggtgc
     4921 gtaagcatgc cgagatgggc gggttcccgg cgaacagcgt ggcgcgagtg tcgacgatca
     4981 tcgatccgac gacatcggag cagagcagct aaggtcgggt cggcggaaaa ccggtcagct
     5041 aaggtccggt tagcaaaact ggctcatggg gagcgattcg gagtttggag cgcgtgatga
     5101 ctttcgcgtt tcgtgactga gatttcgcga ctggaatccg cttcacaggc catagactca
     5161 ttcggatgaa gagatttccg gtagcgcagg gattgccgct ggcgcgtacg caggagcgcc
     5221 tgttgccatc ggggctggtg tgggccgcgg ttgccgtgtt gctggtggtg caggcggtgc
     5281 tggtgctggc gtgtgtgcat cgcgagtcgc tcacgttcga cgaagacgac cacatgtttg
     5341 ccgggtacat gatgtggaag gccggcgact acgggctgaa tcctgagcat ccgccattgg
     5401 tgaagctgct ggcgaccgtg ccggtgcttg gcgagaagat gtggattccg ccggaccagg
     5461 ggcgggagtt caagaaggaa gcttatctgg atggccggga ctggctggcg cggaatgatg
     5521 gggcgagcca gcggctggtg ttccggatgc gcatgatcac gggactgctg gcgtggggtt
     5581 tgtcgctggt ggtgttcttt gcgacgcggg aatggttcgg cgatggcgcg gcactggtgg
     5641 cgatgacact ggcggtgttc gatccgaaca tcatggcgca ctcggcgctg gtgacgaccg
     5701 atgtgggggt gacgctcgga ttcctggcaa cgatctggac gttttaccgc ttcatgcgcc
     5761 agcccacggt gttgcggttg ctggtggccg gcgtggtggc gggattgctg gcggcgacca
     5821 agcattcggg aatcctggtg gtgccgatgc tggcgccggc gatgatctgg gaggtgttag
     5881 ccgcgcagcc gggcgaacgc gcacggcagg cgatgcgcat tgtcagtggc atggtggcga
     5941 ttgtggtggt cgcggcggtg gtgctttggg cgttctacgg gttccggtat gggtcgcggc
     6001 cgccgggtct gctgagcacg agtctggcgg aatatgtgca gccgctgagc gggttcgatc
     6061 aggcggtagt gctgacggtg ggccggcttc atttgctgcc ggagtcgtat ctgatcggtc
     6121 tggcggatgt gaagcggatg gcgcagttct atccgacatt cgcgctgggg aaggtttata
     6181 cccacggggt gtggtggtat ttcccgctgg ccatcttgat caagacaaca ctgggactgc
     6241 tggcgctgaa cgcagtggcg ctgtgggcgg cggtgacggg caagatcggg aaggcgcgcg
     6301 aagtgttcta cgtgctgtcg ccgggtgcgg tgtatctggc gatcgcgatg gcgagcaaga
     6361 tggatatcgg cgcacgacat attttgccgc tgtacgcgct ggcggcgatt tttgcggggg
     6421 ccggagcggc tgcgcttgcg agaggctcga aggcgtgggc cggggtgggc atcgcgctgc
     6481 tggcggtgca tgtggcctcg tcgatggtga attatccgca gcagatggct tatgcgaacg
     6541 aggcgtgggg aggagcgaag aacacacaca atctgctgag cgacgcgaat gtggactggg
     6601 cgcagcagct gtatcaggtg aaggactggc aggaccgtca cccaggggaa gcgtgctggt
     6661 tcgcgtattt cgcacggccg gaggtggatc cggcagtgta tgggatcacg tgccacgcgc
     6721 tgccgaatgc tgacacggga tggctgggag aggggcagga agtgatacct ccggtggtgc
     6781 atggggcggt gctgatcagc gcgggggacc tgagcgggtg tgagttcccg gacgagcgga
     6841 tgaatccgta tcgagagttc atgcatgtgt tgccctcgga ggtgatcgag gacgcagtgt
     6901 ttgtgtatcg cggagagggt ggacagggcg tgcatgtgga gcaggcggca gcgctgagcc
     6961 gggcacagag ggcgagggta ctgctggagc agcatcaggc ggcacaggcg ttggcgctgg
     7021 cgcgggaggc ggtggcgatt gcgccggagg agccgatggc cgggaggatt ctgcaggccg
     7081 cggagaaggc tctcgggaag tagaaggacc gcgaaaagcc ggttcgcttc ggtccggcta
     7141 gctggtgaag cggaaagcgg tgagcgttcg gcgttccgcg atcggcgatc gggaagctgt
     7201 cgcgttacgc gcgttgccca tgcctcaaca cgcggggata ttgggcaccc gcgcccgggt
     7261 gaactgcgtg aagtgaaccg gggcgggatt cgtgcgtatt ctgattcaga gcaactttat
     7321 attgctatgg catcaaggcc gacacagagc gggatggggc ggtacgggcg caaggggccg
     7381 agcttccctg agagcgtggc gcggttctgg aaccgcgtga ccgaggggat gcaggttggg
     7441 gagctgtgga agcagttccg ggccgacgcg cgcaccagct atcggctcta ctcgcaggaa
     7501 gtggatacag cggcaagtgc gggcacgccg cgcgggcggc attttgtgca ggtggcgaag
     7561 cagttcttct gggcgtttct ggaaaagctg acaccggcgc ggcgggtgct attgctggtg
     7621 gcgatcgtcc tgacggtcat gccaggaagc caatggcact ggcattgggg acggagcgac
     7681 gttggatttt cgacgccgga ctttcgcctt tacgggtcgt tgttgatgct ggcgctgctt
     7741 ttgatggaag taggcgatcg cgtggtgatg aagcgcgatc tacagatcgc gaaggagatt
     7801 caggcctggt tgttgccggc cgagccgcca tcagtgccag gtctcgagat tgcgtttgcg
     7861 acgaagccgg cgaacacggt ggcgggcgac tactacgatg tgttcgcgcg gccagggtcg
     7921 gacgggagga cctggctcct tgctgtggcg gatgtggcgg ggaagagcgt gccggcggcg
     7981 atgctgatgg cgacattcca ggcgagtctg aagacgctga gccacacgcc gagcgcgctg
     8041 acggagctgg tggggcggat caatcgctat gcgtgcacga acagccagaa cgggcggcga
     8101 ttcacgacgg cgttcatcgc ggagtatgag ccggcgacgc ggcggatcac gtatgtgaac
     8161 gcggggcaca acaatccgat cctgctgcgg cggtcgggtg cggtggagcg gctggatgtg
     8221 ggcgggatgc cgctgggtgt gatcgatggg gctccgtacg cgtcggggga agtgacgctg
     8281 gaggtggg
//
LOCUS       DASZYN010000284        28524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1166724, whole genome shotgun sequence.
ACCESSION   DASZYN010000284 DASZYN010000000
VERSION     DASZYN010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 28524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28524
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166724"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..2369
                     /gene="tssI"
                     /locus_tag="VHE33_07285"
     CDS             177..2369
                     /gene="tssI"
                     /locus_tag="VHE33_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HVT97296.1"
                     /translation="MSSFSQENRLLRFISPLKGKVLLPERLSGVEGISQLFRYTVDLL
                     SPAGTTIDPAEIVAKSAAIGIRANSAGTERFINGIIAAFELCGGDEEFDNYKAVIVPS
                     VWALTLNKNTRTFQGVTVVDVVGEVLDPYSISPKIDCATDLPPLDYCTQYRETDFHFI
                     SRLMEQHGIFYYFTHTEDSHVLVMQDDSSKLRDCVIQSKFRYAPESGKKEGFYDFVIE
                     AFASRSTMVTGKFTAWDHSFIRNTRLPDPLSSIFSKGPLGENANESYDFADSPAGYFK
                     RRSMDSKIDDEITQILNVRRDASDAGTLVAQGASNAMVLSTGSTFTLEEHPQNSLNAK
                     YLVTHIEHVVQQLPSFRARSAAAPEPYSNTFTAVPFSIPYRAPFVTPKPTVNGMHSGI
                     VVAPKGEDSFADKFGRVCVKFWWDRKRQDHEVDVTLLRVAQSWAGKGWGTYYWPRDGD
                     EVLIDFIEGDPDQPIVVGSVYNGANMPKYDPRGQYTLSGILTRSSKDGGAANANELRF
                     EDLKGKEQIYMNAERDYDLHVESDWHTLVGHAQHTKVGASQIEEIGKNQFVTIHGSEA
                     RKIESVSEINIGQSQTRAIGTSLEETIGDRHVESIGGAAEAVIGGNHIVVVGKDSLQQ
                     IANNSVTQVGQTQSLDAGMNLYLKGGMNVVIEAGMNICLSGPGGFISIGPAGIAIQGT
                     LVMINSGGAPVPIEPVVPPIMGPGPSSPLDPTKPEFPGDDPPSKRKAD"
     gene            2380..3192
                     /locus_tag="VHE33_07290"
     CDS             2380..3192
                     /locus_tag="VHE33_07290"
                     /inference="COORDINATES: protein motif:HMM:NF017315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HVT97297.1"
                     /translation="MGMPACTITSMTAHGGVVVVGFPTVLIGGMPASRIGDMHVCPMV
                     TVLVPHVGGPFILGSPTVIVGGMPQSRVTDMLVCVGPPDVAVMGCPTVLVGMAGCGGM
                     MGAVEGIKAMAVPVLMAAASSAASTLQSELQQDGTMKTSAALGSRLPPITLKQAGWPD
                     LPAKATPNFQSVQPVTLPANTTLFQTVDSSSGSPGSFWSPNPPAGNNAGPSPAASNPD
                     WNDGGKVTSATVPHQGTKAWMGPASSQGSLPGGAPQVYLPPGTVGSSPQVKG"
     gene            3198..3587
                     /locus_tag="VHE33_07295"
     CDS             3198..3587
                     /locus_tag="VHE33_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97298.1"
                     /translation="MSETANIPPALADRFMEAFDAFLASVTTAVTDAEQNPEQASTPD
                     FRAWKNKVLPLLKRQNNEAQQAVEAFRKGDPAPLLAIAQEKRGLAKDLDAFPLTFAGP
                     AHAETLDALETALVTAAYHLCAAAGVP"
     gene            3628..4092
                     /locus_tag="VHE33_07300"
     CDS             3628..4092
                     /locus_tag="VHE33_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97299.1"
                     /translation="MSTQPQIADASAPRIGRWVISTIPPGWTWTPGFGIHKPTGPVTS
                     NICLKDDELLAGNQLDLYIRAQITLLNRTFQEPMIAGPAPFAFPNAAEAALLMVKHRS
                     LQQASVFQVQNYVRIDRWIGVVTLTTIESELLKVRPDFDQFVQRLQIVAPAG"
     gene            4192..5319
                     /locus_tag="VHE33_07305"
     CDS             4192..5319
                     /locus_tag="VHE33_07305"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HVT97300.1"
                     /translation="MAFEPGQRIGDYQIVAQLGAGGLGVVYEVQHLISQRREAMKILL
                     PNQSGTPEMVERFRREVQTLATLNHPNIASLHTAFYHENQLAMVMEMIDGETLRDRRA
                     RIPIPLPEALQYIKQTLHALVYAHALGVVHRDIKPSNIMITRDGLVKLLDFGIAITER
                     STELTHAGYLLGSLSYMSPEQVSGGKATARSDLYAVGVTLYELLTGRLPITGASDYEI
                     MVGHVQQIPPPVHEVAPYISPALSEAVMRALIKDPQQRYGSADVFLQAIEAAVPTGQV
                     PVAFAPPMPGVRSDSGSGSHPSKAMLPQKPGSASGSSGGLQNLPLEEITRRLALYIGP
                     VAKFVIKKIAAQSTDPEYVYQEAARQIASDTDRAAFLRMKH"
     gene            complement(5326..6201)
                     /locus_tag="VHE33_07310"
     CDS             complement(5326..6201)
                     /locus_tag="VHE33_07310"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HVT97301.1"
                     /translation="MKLLAKFNLVLILIFGLGMGLIANNAYNFLMNDAREAVLQQAEL
                     MSANASATKEYTDQQVSPILEKTPEHNDHFLSQTIPFYAANATFQLLRANHPDYSLRE
                     AALNPTNLDDRATEWEADLINFFRDHPAERQHVGQRRTPTGPVLYVAAPIIAESGCLN
                     CHSQPSVAPTAMIDHYGRDHGFGWSRNAIVGAQIVSVPMAVPVAMAKRGFRELLFGLG
                     AIFLLTIILIDLALWLVVVRPLRKVSRNADVISKGEVNMPQLEVKGRDEIAEVTASFN
                     RMHTSLLKAFEMLNG"
     gene            6378..7403
                     /locus_tag="VHE33_07315"
     CDS             6378..7403
                     /locus_tag="VHE33_07315"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HVT97302.1"
                     /translation="MRAAELIAPLTFRLTEMPIDEPGPGEVQVHVEAVGVCGSDLHAW
                     SEGAVGSTPNVYPMVLGHEPAGTIVKAGAGVSGFTPGDRGALEPALYCYHCEFCLSGH
                     HNVCANIRFLSNPHHPGFFREFVNLPVANFRPIPATVSFEEAALAEPLAIGIHSAQLT
                     SIRPGETVAVIGAGPIGLVTIAALRAAGAERVWAVEPLAHRRELARAIGADAALEPAE
                     AVAEILHATGQRGVDCAIDCAAGEETTSQAIQLARNAGRVALTGIHSTPFVAMDGSAM
                     RRKELTIFNVRRSNHETEKAIRLLEAHPGWFRPLLTHTRDIDHIDEAFTIAGQYRDNV
                     GKMTIRP"
     gene            7634..11020
                     /locus_tag="VHE33_07320"
     CDS             7634..11020
                     /locus_tag="VHE33_07320"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HVT97303.1"
                     /translation="MGYFRRSVLAASLLLGAGLAVSAQNSPDSMKSGFENPPESARPR
                     VWWHWMNGNITKEGIQLDLDWMHRVGIAGFQNFDAALQTPQVVPHRLAYMTPEWKDAF
                     KYAIETGDKFGMEMAIAGSPGWSESGGPWVPPSEGMKKYVWSATDVEGGQPFTGTLAH
                     PPETTGAFQDIGIRDALAGPEGGKPEPHFYADSVVVAYRRPASDVNVESLHPAMTFSG
                     GSPDMALLTDGDLEKAAELPIPEQGQKAWIQYAFSEPTAIRAVTIVTQDPNRIITMLT
                     GIGAPNKALEASDDGETWRTVTELGTSDTPELTVSFPEVKAKYFRVTFLRTPPPPRPA
                     WAEGIDPSSFGLKLPPKPTNYEIAELVLHPGARVNHFEQKAAFVPEPDLYGFATARID
                     QGDAVNKSDVIDLTSKMKPDGSLQWTPPPGRWVILRFGYSLLGITNHPATAEATGLEV
                     DKLNGKDVRQYMEKYLDSYKETVGADQMGQRGIRYIINDSWEAGSQNWTDDMIAEFRK
                     RRGYDPIPWMPVLAGEIVQSAQASDQFLWDFRKTIADLIADEHYGTLEQVIHEWHMKH
                     YGESHEDGRAFVADGMEVKKFNEVPMSAMWTQSPGVNHEQFGYNADDRESASVAHIYG
                     QNLAAAESMTAAAAPWAWSPATLKPTADQEFLNGINRFVIHESAHQPLVDKKPGLTLG
                     PFGQWFNRNETWADEAGAWVNYLGRTSYLLQEGHFGADVTYFYGEDSNLTAIFHDKAP
                     DMPPGYGFDYVNADALIHVLNVADGRITTPGGTTYRLLVLDPYSKHMSLPVLRAIDKL
                     VREGASVAGPKPTDDPSLADDQAEFHRLNDALFGDGTGTHSVGKGTVYAGDDATAALK
                     AMNVPPDFHYTGSKNRLEFVHRRLASGDLYFVDNRSDDSAAVQATFRVTGKAPELWYA
                     ETGQTKPVSFTIADGRTTVPLELEPWGTVFVVFRKATSETSHTLPAVTEKQMATVSGP
                     WSLSFPPNWGAPPSVTLDTLASWSENSDAGVKYFSGTGTYTKTIDAPASWFSKGAHVW
                     IDLGDVKNLAEVMVNGKSLGIVWHAPYRVDATTALKPGSNRLTVKVTNAWVNRMIGDQ
                     QPDAAKKYTFADVKPYKATSPLLPSGLLGPVTIVEESAAR"
     gene            complement(11136..13364)
                     /locus_tag="VHE33_07325"
     CDS             complement(11136..13364)
                     /locus_tag="VHE33_07325"
                     /inference="COORDINATES: protein motif:HMM:NF026221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HVT97304.1"
                     /translation="MQSSRRTFLKHAAGLSAACLVGFDDAAGNAAAAELRSSSKNSQA
                     GSWYDRPMRWAQLSFVEDDPGHYDLAFWLDYFRRIHADAALLDAGGCVAFYPTKIPLH
                     YRSQWLGDRDAFGEISAGCRSMGMNVVARTDAHACHQDVYDAHPDWLMVDEHGNQVRH
                     PSDKDFWLTCALGPYNFEFMTAVHQEIMGLYKPDAIFTNRWAGSGMCYCEHCRANFHQ
                     FSGLELPRTRDPHDPARRQYMVWRQERLFDLCRLWNSTIQAIHPNACYLPNAGGGALS
                     ELDMKTFAEISPAAIADRQARRGLMAPWMAGKSAKEYRSTMGMKPVAGLTSVGLDDRN
                     RWKDSVQDGEEIRLWMVDGIAHNFRPTFTKFNAKPFDKRWFPIVEQVFQWHFANERYL
                     RNERSLARVGLVYSQQTAASYGWPDATSRVEDPELGFYEALIEARIPFEMVHDGLLDE
                     ENLGQFRTLILPNIAVLSNRQCDQIRAFVRKGGGIVATSETSLYDERGGRRSDFGLAD
                     LFGASFAGKVEGPMLNSYLSLKGDPKTGEYHALLAGLEDAGRIINGTHWVHVETHDPD
                     SYSPLEVVPPYPDLPMEEVFPRATGDRAPGVFLREIGRGRSVYFPVDIDRTFWDVLAT
                     DHSKLLRNAVLWATHEPAPLEVEGKGILDVAIWSQKGSVTAHLVNLTNPMMMKGPVRE
                     VIPISRQVVRIRIPNDGRRVTKASRLVAGGEISFQEDSGAIVIEVPSIELHEVIALDL
                     EG"
     gene            13486..15180
                     /locus_tag="VHE33_07330"
     CDS             13486..15180
                     /locus_tag="VHE33_07330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HVT97305.1"
                     /translation="MFETASAWHQTYSLFGSSLGVSALIAAVPIFVFLLLLGVLRKPA
                     WMAGLVGLGAAFALAVGGYGMPLIPAASAALYGAAFGLFPISWIIFWAIALFRVTSES
                     GKFEIIRGSIGRLTPDPRLQALLIAFAFGAFLEGAAGFGTPVAIAATMLSGLGFSAFS
                     ASALCLLANTAPVAFGAIGIPVITLAGITGLPLDKLSSAVGMLCSPVALFIPLYLIVA
                     MAGFRALSGIVLPTLVAGASFAIVQYLVSTFMGPQLSALLAAIVSMLSLILIVRLRRR
                     RLKDRDTTFSEVPGADLRTVGTDGSSAPPQKASGLAKEHSAREVAVAWMPYVFLVGCV
                     ILWGVAPIHQALNRATLNFNWPGLHDLVLRMPPVSKAPSPYHAPFSLNFISAAGTACM
                     VATFLSAFYLRIGLPGLVGALTRTTKQLAVPIATVALVLAVGFLMNYCGATATLGLAF
                     ASTGRLFPFFSPLLGVLGVFLTGSDTSSNALFGNLQVVTASRLGLDPVLMAAANASGG
                     VMGKMISLQSIAVAAAATRLSNHDQGRLFRFTLKHSLFLAFVIGCLAMLYAYRLHL"
     gene            complement(15134..16180)
                     /locus_tag="VHE33_07335"
     CDS             complement(15134..16180)
                     /locus_tag="VHE33_07335"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HVT97306.1"
                     /translation="MPKCVGRFLIVNVLVATAALAGAAPHRSGVIDPGSIWLDNRGRE
                     IQAHGGGILHLGKTYYWFGEDRSQDNDPGMGYVTCYSSTDLAHWRFRGQVFKLANPED
                     PGSPFVLERPKVFYNQRTKRYVMYMHFDDSKYKLARVGVAVSDKVDGPYRYLRSFRPL
                     GQESRDIGQFVDDDGSAYLIFESRPTHGFFIAKLSDDYLNVEKEVSFIPVPLEGGALV
                     HYKGFYYVLGSHLTGWHPNPNVYAVAPTLAGPWTAMKDIAPPETNTYDSQSSMLIKVD
                     GKETETVIYMGDRWNPQKLWDSRYIWMPLEMGDGTLHLPEPRPWSIDIKTGEITKLGS
                     TDAGDRRRASPDTQ"
     gene            complement(16201..17619)
                     /gene="glcF"
                     /locus_tag="VHE33_07340"
     CDS             complement(16201..17619)
                     /gene="glcF"
                     /locus_tag="VHE33_07340"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HVT97307.1"
                     /translation="MSGLVNLAEPKRSSFDGDHPPDRDLLADCVHCGFCLPACPTYVL
                     WGEEMDSPRGRIYMMQKAADGAAQLDEMFKAHMDRCLGCMACMTACPSGVQYGKLIED
                     TRAQVERNIPRAASDSWFRRLLFATFPYPSRLRVFAGAVRLYQRSGLQRVLHRMGLLN
                     LLPRKVAAMEALLPTVPAEYRATMPVRTPAVSKPRRRVGMLAGCVQQVFFQQVNAATV
                     RVLSAEGCEVIVPSEQECCGALMLHAGLKRDAAAMAKKMIAAFESAEVDTIVINAAGC
                     GSTMKEYGYLLRDDLVWAERARAFSAKCRDIAEVLCEMEPIATRNPLKLRVAYHDACH
                     LRHAQGIYREPRQILSAVPGLEIAEIAESLLCCGSAGVYNVLQPEAAEQLGDRKVANL
                     LETQAEAVVSANPGCLLQLMSGLRRRGERAMPAFHLVELVDASMRGVEAAELLRSAAP
                     AFDKTEAQNSGSHVSRRKGSRL"
     gene            complement(17616..18788)
                     /locus_tag="VHE33_07345"
     CDS             complement(17616..18788)
                     /locus_tag="VHE33_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HVT97308.1"
                     /translation="MEHLTSRFEEFARTIGAEIVSERDGRVVASPRSTEEVAMVLQNA
                     EANGFGVEIVGAGTKRRWAGAVRGDVVLETRGIAGVREHSWQDMTATVGAGTTWMEMQ
                     RVLAEHGQMVALDPLWPERATVGGVIAANDSGAFRLRYGSLRDLVIGMTIVLADGTIA
                     RSGGKVVKNVAGYDLHKLMIGAFGTLAVVTDATFRLHSIPRNSRTWSFESTDAVACGQ
                     MLIKVLDSQLRVQAMQLRANAAGFALDVAIASIDEVMREQWSLLDSLAGCAGAIPGDV
                     FAARERLFTGNGVVAKVTMLASAIARMSAEVVRLGGEAVTQASGIMVARLADASTVDR
                     LRELVAAEGVGAVTVLRGEDGYGVLPPGGDAGRLMREIKRQFDGKGLLNPGVVIGG"
     gene            complement(18788..20236)
                     /locus_tag="VHE33_07350"
     CDS             complement(18788..20236)
                     /locus_tag="VHE33_07350"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HVT97309.1"
                     /translation="MLRDSIIDELRAAVPGGLITAASELQTYECDGLTNFRTMPGAVV
                     LPQSAEQVQSVVRICARHRIPFVARGSGTGLSGGALPAEDSVVISFARMNRILSIDIP
                     NQRVVVEPGVINLHVTEAVAPYGYFYAPDPSSQSVCTIGGNVAENSGGAHCLKYGFTT
                     THVVGMEVVLPDGELVHFGSATPDPPGYDLTGLFVGSEGTLGVATKLTLRIVKRPETV
                     QVLLAAFDSIRAAGQTVSDIIAEGMLPAALEIMDRLSVKASEAAVHPNYPVCEALLLV
                     ELDGPAAEVTAQIAAATSLCENNGAREVRRAQTADERALVWKGRKAAFAAVGRISPNY
                     IVQDGVIPRTALPDVLEDISRMADAVNLRIANVFHAGDGNLHPLVLYDKAVAGQEEIA
                     LDLSYRILRACVDRGGSITGEHGVGREKQEAMGYMFSEPDLATMQRVRIAFDPQNLSN
                     PDKVFPRTRLCGEKTGPYTPHPLERAGVAEFF"
     gene            complement(20404..21246)
                     /locus_tag="VHE33_07355"
     CDS             complement(20404..21246)
                     /locus_tag="VHE33_07355"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HVT97310.1"
                     /translation="MRKLSHRSYKSTSFLEGNGKEDVTSLLVKRFQEMLRDGLLRQGS
                     RLPPERDLAAHFNVARSSLRQALKVLEVMGVITQKVGDGSYLNTDSSAILAVPMEFLF
                     LLDDTSVGELTELRLLIEPGLARLAAERATAEDIALLRQSIHDFESSGGDNLKIVSSD
                     LLFHRAIFQASKNGTATSVFHSIHRAMAKMILVTSQLVDLEHTLAFHKPIMQAIERRD
                     GNRAARLMAEHLTDATAILTHEQEHQHQLLLQDRIVRERRPLLRGRKPAKKQPRNKAK
                     AASQ"
     gene            21446..24778
                     /locus_tag="VHE33_07360"
     CDS             21446..24778
                     /locus_tag="VHE33_07360"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HVT97311.1"
                     /translation="MSSYCVVCGRSARVVVVLLFLALSLIAHGQVEQGQFVGRITDPS
                     GAAIAGAQLEARNVETNITYKAVSNGSGDYVITPVPSGNYVITVVAQGFQQAATKPVE
                     LQVGQVARVDLPLTIGTTTTVVNVNTSAPLLSTDSATIHQVISNRQVLDLPLNGRGYF
                     QLAELTPGTALLAPTGNSLAIRPEVVNGNVISGIRGRATSFLMDGVDVTEQHQGGTFI
                     QTSIDALQEFSVEQSPYSAEYNRGGAFFNATIKSGTNSWHGGVFDFLRNDAFDARNFF
                     ALQRQRLKRNQFGGDVGGPVSIPHLYKGKDRTFFFFSYEGQRLSEGLVFNGIVPSDAQ
                     RGGNFGSKPIYDPESSVTTGNVTTRQQFTDNIIPQDRISQRALAILGYYPKQNTTTGT
                     FSAVPAQTINFDQYIIRLDDQLTTNNRLFAHWIYMTQHEVDPNFSPALGRANLTSIGQ
                     DIAVGLTTSLGSTMINQARAHYLPSHVRLQAFLQGNDFNGDFGVGGFSGLLRNGTSSF
                     PDYSWTGYASLQGSTFDQRPKSQDRKAVEFSDNFTKILGRQSLKAGILVRYYQWLGYD
                     SGTYAGLFSFNGNETAQVTVTQNSDGTKTTTASGGDAFADFLLGYPSAVQRSYPGYNF
                     GGQGWSKQFYVQDDIRISDRVTLNAGLRYEYTPWLNGYRGQVGTFDPTLSKPIIDSGK
                     GSTLDLSAQPGAPAAYQYFGQYIQTSSEAGLPSNISYNDTRQFGPRVGVTVSLDPKTV
                     VRAGFGIFYEPENTDGRLNLNMLPFRLNETQNQTQNTTPTRSLSDFFLGAPLGSALAN
                     PSLVPTKVHMSMGRNAHYSLDVQRRFSDHDMLDVGYVGNRSLHLNATNDFNDPAPGPG
                     SVQSRRPYQPWSGITFDTQDMSSNYNALQAKYEHRFSHGFNLLASYSWSKWLQFNQAP
                     ALGGNTGYEYARAASDIPQNLAVSGIYLVPFGRGRHFLTNGNGLVDGILGGWQLQAIV
                     DQRSGIPFTPIVSGDRANTGVGSQRPNLNPAGCSAGFHRSLTTWFNKSCYVDGPVYTY
                     GQVHANTLRADVYRQYDASIFKNFSLPRESTLSLRAEFFNVSNTTSFNSPNATVDVAA
                     GGRVTATSNQPREIQLALKYSF"
     gene            24836..26563
                     /locus_tag="VHE33_07365"
     CDS             24836..26563
                     /locus_tag="VHE33_07365"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97312.1"
                     /translation="MQAIVRRLMPLSALLTLVVPLAQGQGVIPPPRPAPDQPTVSINV
                     DLSQKIAPYQPIYSWFGYDEANYTTTNLGRALTHELADLSPVPVHIRVHHLLTSGNGE
                     PELKFSSTNVYHEDASGNPVYDFTILDNIFDAYKAAGVEPMVELGFMPKDLAAVLPNR
                     NEPYQVHYPHSTVSGASNNPPKDYKKWGDLAKAVTAHLVERYGREQVQKWDFEVWNEP
                     DIDYWHGSREDYFRLYDYAVAGVRAAFPAARVGGPATTGPSSQRAADFLKAFLDHVAN
                     DKSAATGGAIPLDFISFHVKGHPTIAGGDVTMGLNHELSDADHGFSIIAQYPAFRKLP
                     VILSEADPEGCAACSSRVNPANNYRNGTLYPAYTATAFKGLLDLAQQHKTNLISMLSW
                     SFEFEGKDYFEGFRSLSTNGIDKPILNLFRMLGMMSGTRVTASSSGSVPLQTQVETGV
                     RASADVDALATRHDRGAEVLVWNYHDEDQPAPDTPTQLTIRGIPPGVQRVLLQNYRID
                     ATHSNAYTIWKQMGLPQQPTTEQYAKIRAEEGLQLLDSPQWLNVSDGAVRISTLMPRQ
                     SVSLLALSW"
     gene            26583..27821
                     /locus_tag="VHE33_07370"
     CDS             26583..27821
                     /locus_tag="VHE33_07370"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT97313.1"
                     /translation="MARKWYFVFLVSIAIAISYFDRLTLPVAIAAIQHNIPLSNEQYS
                     WLQTAFLLSYAALYAIGGRLLDWLGTRRGFLLIMLWWSLACALHSLASGFFLLVVARF
                     LLGMGEGGAFPAATRVVAEWIDAPERSTAMGLINAGTAIGSLLAPPIIGFILLRSGWR
                     AVFIAAGAAGLAWGFWWALSYRVGAPGAVGSGDPGTPAGEVRWLDVLRLPNVQAFVFA
                     KFMSDSAWYFLLFWLPKYLYDARSFDIRQVSYYAWIPYAASGVGSFLGGWFSSTLLHR
                     GSSLDAARKIALGCCAAAMPVVMLVSLVPVSMALTLFSIAFFAQQAWSGLVMTLPADI
                     FPLNMVGTVAGLIGFGGAMGGAIFGVVAGFLLGHGTSYGVLFVAVGTFHLIGFLAIFL
                     RGGRIQPLRAMDIKSIGSAA"
     gene            27818..>28524
                     /locus_tag="VHE33_07375"
     CDS             27818..>28524
                     /locus_tag="VHE33_07375"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HVT97314.1"
                     /translation="MKITEVRSRVVQWEGPVVPLPPHFCTNPMDLVSFRQASMGNFAF
                     HNWLLVEIFTDTGLVGLGNAALSPLVTKQLLDIYLKPLLIGADPWDVEYLWQQMYRRT
                     LAFGRKGVAMAAISAVDIALWDLLGKDAKQPVFRLLGGRTKDKIPVYASRLYAMPLDE
                     LRAEAQKYKDQGYTMMKLRFGYGPLDGAVGMQKNIELVRTVRDVIGEGIDLMTDAYMG
                     WTLDYAKRMLPRLEPFNL"
BASE COUNT         5706 a         9199 c         7920 g         5699 t
ORIGIN      
        1 gaaatttccc gcgaagttct gtcccgcatg gctcaggaag cccccaccga gcgcgttcac
       61 gtcggcgtcg gtccggaggg cggctttacc tatcagtggt agttacgttt tctgcgtgag
      121 cgcggccgcc gtcgaatcga ctgcgtctcc tcgctgtcag ggagagatcg aaatctatga
      181 gctcattcag ccaggaaaac cggcttctgc gcttcatcag cccgctcaag ggcaaggttc
      241 tccttcccga gcggctttcc ggcgtagaag gcatctcgca gctttttcgc tacaccgtcg
      301 atctgctgtc ccccgccggc acgacaatcg atcctgccga aattgttgcc aagtcggctg
      361 ccatcggcat ccgcgccaac agtgccggca ccgagcgctt catcaacggc atcatagccg
      421 cgttcgaact ctgcggcggc gacgaagagt tcgacaacta taaagccgtc atcgtgccca
      481 gcgtttgggc tctcacgctc aacaaaaata cgcgtacgtt tcagggcgtg accgtggtcg
      541 atgtggttgg agaagtgctc gacccctaca gtatttcccc gaaaattgac tgcgcgaccg
      601 atctcccccc gctggactat tgcacgcaat accgcgaaac ggacttccat ttcatctcgc
      661 gcctgatgga gcagcacggc atcttctact acttcacaca caccgaagat tcccacgttc
      721 tcgtaatgca ggacgactca tccaaactcc gcgactgcgt gattcagagc aaatttcgtt
      781 acgcgcccga gtccggcaag aaggagggtt tttacgactt tgtcatcgag gcttttgcct
      841 ccagatcgac catggtcact ggcaaattca ctgcctggga ccacagcttc atccgcaata
      901 cgcgcctgcc cgatcctctt tccagcatct tcagtaaagg tcctttgggc gaaaatgcga
      961 atgaatccta cgactttgcc gacagccccg cgggatactt caagcggcga tccatggatt
     1021 cgaagattga cgacgagatc acccagattc tcaacgtccg tcgcgatgcc agcgacgccg
     1081 ggactctcgt cgcgcagggg gcctccaatg cgatggtgct gtccaccggt tccactttca
     1141 cgctggaaga acacccccag aactcgctca acgcgaagta ccttgtcaca cacattgagc
     1201 acgtcgtgca gcagcttccg tccttccgcg cacgctcggc tgccgctcct gagccctaca
     1261 gcaacacctt cacagccgtg ccgttctcaa tcccgtaccg cgccccgttt gtgacgccaa
     1321 agcctacggt gaacggaatg cacagcggca tcgtcgttgc tccaaaaggc gaagattcgt
     1381 tcgcagacaa gttcgggcgc gtttgcgtga agttctggtg ggatcgaaag cgccaagatc
     1441 atgaggtcga tgtcaccctc ttgcgggtcg cccagtcctg ggcgggcaag ggctggggta
     1501 cctactactg gccgcgcgat ggtgacgaag tccttatcga cttcatcgag ggggaccccg
     1561 atcagcccat cgtggtcggg tccgtctata acggcgccaa catgcccaaa tacgacccgc
     1621 gcggacaata tacgctctcg ggcattctca cccgcagttc gaaagacgga ggagcagcca
     1681 acgcgaatga actgcgcttc gaggatctca agggcaaaga gcagatctac atgaacgccg
     1741 aacgtgacta cgatctccat gtcgaaagcg actggcacac cctcgtcggc cacgcgcagc
     1801 acacgaaggt cggcgccagc cagatcgagg agatcggcaa gaatcagttt gtcaccatcc
     1861 atgggtctga agcccgaaag atcgaatccg ttagcgaaat caatatcggg caaagccaga
     1921 cccgggccat cggcacgtcc ctcgaggaaa cgattgggga caggcatgtg gaatccatcg
     1981 gaggcgcggc cgaggctgtg atcggtggaa accacatcgt cgtcgtcggg aaagattctt
     2041 tgcagcaaat cgccaataat tccgtcaccc aggtgggcca aacgcaaagc ctggacgcgg
     2101 gtatgaatct ttacctcaag ggcgggatga atgtggtcat tgaagccgga atgaacatct
     2161 gcctctcggg cccgggagga ttcatcagca ttggcccagc cggcattgcc atccagggaa
     2221 cgctcgtcat gatcaacagc ggtggcgccc cggttccaat cgaaccggtc gttccgccaa
     2281 tcatggggcc agggccttcc tcccccctcg atcccaccaa gccggagttc cccggcgacg
     2341 acccgccgtc aaaacgcaaa gctgactgag agacacacca tgggaatgcc agcctgcacg
     2401 atcaccagca tgaccgctca cggcggagtc gtcgtcgtcg gatttccaac cgtcctgatc
     2461 ggcgggatgc ccgcgtcgcg catcggcgac atgcacgtgt gccctatggt gactgtcctc
     2521 gtaccgcatg tcggcggccc cttcatcctc ggttcaccca cagtgattgt cggcggcatg
     2581 ccgcagtcgc gcgtcaccga catgctggtt tgcgtaggcc ctcccgatgt tgctgtgatg
     2641 ggctgcccaa ccgtactcgt cggcatggcc ggttgcggcg gcatgatggg tgcggtcgag
     2701 gggatcaaag cgatggccgt tcccgtcctg atggccgctg cttcgtcggc agcatccacc
     2761 ctccagtccg agctgcagca ggacggaacc atgaaaacgt cggccgccct cggcagccgt
     2821 ctgcctccca tcaccctgaa gcaggcgggc tggcccgatt tgcccgcaaa ggctaccccg
     2881 aactttcagt ctgtgcagcc ggtcactctg cccgccaaca caacgctttt ccaaactgtg
     2941 gatagtagtt ccggttcccc cggcagcttc tggtccccca atccgcctgc cggcaacaac
     3001 gccggaccct cgccagctgc gtcgaacccc gactggaacg atggaggcaa agtcacatcc
     3061 gcaacagttc cccaccaggg caccaaagcc tggatgggtc ccgcctccag tcagggaagc
     3121 ctccccggcg gggcccccca ggtctatctc cctcccggaa ccgtcggttc ttctccacag
     3181 gtgaagggat aacgcccatg agtgagacgg ccaacatccc cccggcgctg gcggatcgat
     3241 tcatggaagc cttcgacgcc tttctggcat ccgtaacaac tgccgttaca gatgcggagc
     3301 agaatccgga acaggcatcc accccggact ttcgcgcctg gaagaacaag gttctccccc
     3361 tcctgaagcg ccaaaacaat gaggctcagc aggctgtcga agccttccga aaaggcgacc
     3421 ccgccccgct tctcgcaatc gcccaggaga aacgcggcct ggcgaaggat ctggatgcgt
     3481 ttccgctgac cttcgcggga cccgcgcacg cggaaacgct cgacgcactg gaaacagccc
     3541 tcgtcaccgc cgcgtatcat ctctgtgccg cggccggagt tccatgaccc tgtcacgaca
     3601 aggaactgag gctccacgct ttctgcaatg agcacgcagc cacaaatcgc tgatgcatcc
     3661 gccccgcgta tcggccgctg ggtgatttcc acgattcccc ccgggtggac gtggaccccc
     3721 ggctttggca ttcataagcc caccggaccg gtcacaagca acatctgcct caaagacgac
     3781 gaactgctgg ccggcaatca gcttgacctc tacatccgcg cgcagatcac tcttctcaac
     3841 agaacattcc aggagccgat gatcgcaggg cccgcgccct tcgccttccc caacgccgcc
     3901 gaagcggcgc tgctcatggt gaagcaccgc tcgctgcagc aggcctcggt cttccaggta
     3961 cagaactacg tacgcatcga tcgctggatc ggcgtcgtaa ccctgaccac gatcgaatcg
     4021 gagttgctga aggtacgccc cgacttcgat cagttcgtgc aacgactgca aatcgtcgct
     4081 cctgcaggat gaaaccgccc ctgcccgcca cagcgccaga gacgccgccc gggagcgggc
     4141 gctatcatgc aaaagtgcct cgtcttctct tctggcacac caacgttgca catggcattt
     4201 gaaccgggac aaagaatcgg cgactaccag atcgtggcgc aactgggcgc gggcggcctg
     4261 ggcgttgtct atgaggtgca gcacctcatc tcgcaacgac gcgaggcgat gaagatcctg
     4321 ctgcccaacc agtcaggcac accggagatg gtcgagcgct tccgccggga agtacagacc
     4381 ctcgcaacgc tgaaccatcc caacatcgcg tcgctgcaca ccgccttcta ccacgagaat
     4441 cagctggcca tggtcatgga gatgatcgac ggcgaaacgc tccgcgaccg gcgcgccagg
     4501 attccgatcc cattgcccga agcgctgcaa tacatcaagc agacgctcca tgctttggtt
     4561 tacgcgcacg cactcggtgt cgtacatcgc gacatcaagc cgtcgaacat catgatcacc
     4621 cgtgatggcc tcgtcaagct gctggacttc ggcatcgcca ttaccgagcg ctcgacggag
     4681 ttgacccatg ccggatatct gctcggctcg ctcagctaca tgtcgcctga acaggtgagc
     4741 ggaggtaagg ccacagcccg ttccgatctt tatgccgtag gcgtaactct gtatgagttg
     4801 ctcactggac gcctgcccat caccggcgcc agtgactacg agatcatggt cggacacgtg
     4861 cagcagatcc ctccacccgt tcatgaagtg gccccgtata tttcaccagc gctctccgaa
     4921 gctgtgatgc gcgcgctgat caaggatccg cagcaacgct acggctcggc cgatgttttt
     4981 ctgcaggcga tcgaagccgc tgtccccacc ggccaggtgc ctgtggcatt cgcgccgcct
     5041 atgcccggcg ttcgctctga ctctggttca ggatcccacc catcgaaagc aatgcttccg
     5101 caaaaacctg gatctgcgag cggatcgtcc ggcggattgc agaacctgcc tctggaagaa
     5161 atcacgcgcc gcctggctct ctacattggt ccggtggcga aattcgtgat caaaaaaatc
     5221 gctgcacagt cgaccgaccc cgagtacgtc tatcaggaag ccgcccggca gatcgcctcc
     5281 gacaccgacc gcgctgcttt tctccgcatg aaacactagt cgcgctcatc cgttcagcat
     5341 ttcaaaagct ttcagcagac tcgtgtgcat gcggttgaac gacgccgtca cttcggcgat
     5401 ctcgtcgcgt cctttcacct ccagttgagg catgttcact tcgcccttgc tgataacgtc
     5461 tgcgttcctc gaaaccttcc gcagcggccg aacaacgacc agccacagcg ccagatcaat
     5521 aaggatgatc gtcaaaagaa aaatcgcgcc cagaccaaac aacagctccc gaaagcctcg
     5581 ctttgccatc gcaactggaa ccgccattgg cactgaaacg atctgcgcac cgacgatcgc
     5641 gtttctcgac cacccaaaac cgtgatcgcg gccgtagtga tcaatcattg cggtcggcgc
     5701 aaccgacggc tggctgtgac agttcaggca tcccgactcg gcgatgatcg gagcggcgac
     5761 gtacagcacg ggccccgtgg gcgtccgccg ctggcccaca tgctgtctct ctgccggatg
     5821 gtcgcggaag aagttgatca ggtccgcctc ccattccgtc gcgcgatcgt ccagatttgt
     5881 cgggttcagc gccgcttccc gcaaagaata gtccggatgg ttcgcacgca gcaattgaaa
     5941 ggtggcattc gcggcgtaaa acggaatcgt ttgcgacaga aaatgatcgt tgtgctccgg
     6001 agttttttcc agaatcgggc tcacctgctg atccgtgtac tctttggtcg ccgacgcatt
     6061 ggccgacatc agttcagcct gctgcagcac ggcctcccgc gcgtcgttca tcagaaagtt
     6121 gtaggcgtta tttgcgatga ggcccatccc cagcccgaaa atgaggatca gcaccagatt
     6181 gaatttggca agcagtttca ccagcgcctc cgaaatcgaa accatcatat ctgcgcggac
     6241 ctgccgatcg ctccctcgtt tgggcaggtc gcgtcggcgt tctcatcgtc agccgggtct
     6301 cctgaacatg cgttctcctc tgttcgctga tcccaccgtc cctgcgatgc gatatcgttg
     6361 ctgcaggatc accctcgatg cgcgctgctg aactcatcgc cccgctgact ttccggctga
     6421 cggaaatgcc gatcgacgaa cccggaccag gagaggtgca ggtgcatgtc gaggccgtcg
     6481 gggtgtgcgg gtccgatctg cacgcctgga gcgagggcgc tgtcggcagc actccgaacg
     6541 tctatcccat ggtccttggg cacgaacctg ccggaaccat tgtcaaagcg ggagccggcg
     6601 tttcgggatt cactcccggg gaccgcggcg cattggagcc ggctctctat tgctatcact
     6661 gcgagttctg cctcagcggc caccacaacg tttgcgccaa tatccgcttc ctgagcaatc
     6721 cccatcatcc ggggtttttt cgcgagttcg tcaaccttcc cgtcgcaaac tttcgcccca
     6781 tccctgcaac cgtgtccttt gaagaggcgg ccctggcgga gcctctggcc attgggatcc
     6841 attcggcgca gcttacctcc attcggccgg gagaaacggt ggcggttatc ggtgcaggac
     6901 cgattggact ggtaaccatc gccgcactgc gcgccgctgg agcagaacgc gtgtgggctg
     6961 tcgaaccgtt ggcgcaccgg cgcgagttag cccgtgcgat cggcgccgac gcagctctcg
     7021 agcctgccga agccgttgca gagatcttgc atgccacggg acaacgcggt gtcgattgcg
     7081 cgatagattg cgcggctgga gaagaaacca ccagccaggc aattcagctc gcgcgcaacg
     7141 ccggccgcgt ggcgctgacc ggaattcact ccaccccctt cgtggcgatg gacggttcgg
     7201 ccatgcgccg taaggaactc accatcttca atgtgcgccg ctcgaaccac gagaccgaga
     7261 aagccatacg gttgctcgag gcccatcccg ggtggtttcg acccctgctg acgcatactc
     7321 gcgacatcga tcacattgat gaggcgttca cgatcgccgg ccagtatcgc gacaacgtag
     7381 gaaagatgac gatcaggccg taagcgcccc aggacgcatc gcgaatacgt tcaggtacca
     7441 gaaacagccg gaatcatcgt cagctggcct cgaccgtcgc ggcgaagcgc ggctccgaac
     7501 ttcaatgggg aatcagttcc cctttcgaat ctcgttcgtg ttagtgtgtt tttcgcattg
     7561 gaaaccggct ccgggaaccg gagtttcgtc cctgacaatc ccggtgggcg caatccagct
     7621 gaaaggactc aacatgggct attttcgacg ctcagttctc gcggcaagtc tgctgctggg
     7681 agccggcctt gcagtttccg cacagaattc acccgattca atgaaatccg gatttgaaaa
     7741 tccacccgag agcgcgcgcc ctcgcgtgtg gtggcactgg atgaacggca acattacaaa
     7801 ggaaggcatc cagctcgatc tcgactggat gcaccgcgtc ggcatcgccg gcttccagaa
     7861 ttttgacgct gccctgcaga caccgcaggt ggtgccgcat cggctggcct acatgacgcc
     7921 ggagtggaag gatgctttca aatacgcgat cgagaccggc gacaagtttg gcatggaaat
     7981 ggccatcgca ggctcgcctg gctggagcga gtccggtggt ccctgggtgc ctccttcgga
     8041 aggcatgaag aaatatgtgt ggagcgcgac cgacgtagaa ggtgggcagc ccttcaccgg
     8101 caccctggcc catccccccg agaccaccgg cgcttttcag gatatcggaa tccgcgatgc
     8161 gctggctggc cccgagggcg gcaagcccga accgcacttc tatgccgatt ccgtcgtggt
     8221 ggcctatcgc cggccggcca gcgacgtgaa cgtagaaagc ctgcacccgg ccatgacctt
     8281 tagtggagga tcgccggata tggcgctgct gactgacggc gacctggaga aggctgcgga
     8341 actgccgatc ccggagcagg gacagaaggc gtggattcaa tatgccttct cggaaccgac
     8401 ggcgattcgc gctgtcacta tcgtgacgca ggatcccaat cggatcatca ccatgctcac
     8461 cggcattggc gcgccaaaca aggcgctgga ggcgagcgat gatggagaaa cctggcgcac
     8521 cgtcacggaa cttgggacgt cggatactcc ggagctgacc gtgtcctttc cggaggtcaa
     8581 agcgaagtat ttccgcgtga ccttcctgcg cacgcctccg ccgccgaggc cggcttgggc
     8641 tgaagggatc gatccgtcct cgtttgggct caagcttccc cctaagccta cgaattacga
     8701 gatcgcagaa ctggtgctgc atcccggagc gcgggtgaat cacttcgaac aaaaagccgc
     8761 gttcgtgccg gaaccggatc tgtacggttt tgcaacagct cgcatcgatc agggcgatgc
     8821 cgtcaacaag tccgacgtaa tcgatctcac ctcgaaaatg aagcccgatg ggtcgctcca
     8881 gtggacgccg ccgcccggcc gctgggtcat cctgcggttt ggctacagcc tgctgggcat
     8941 cacgaatcat cctgcaaccg cggaggcgac gggccttgag gtggacaagc tcaacggtaa
     9001 agatgttcgc cagtatatgg agaagtacct cgacagctac aaggaaaccg tgggcgccga
     9061 tcagatgggc cagcgcggca tccgctacat catcaacgac agctgggaag ccggctcgca
     9121 aaactggacc gatgacatga tcgcggagtt ccgcaagcgc cgcggctacg atccgattcc
     9181 ctggatgccg gtgttggccg gagagatcgt gcagagcgca caggccagtg accagttcct
     9241 ttgggacttt cgtaagacca tcgcggacct gattgccgac gagcactacg gaacgctgga
     9301 gcaggtcatc cacgaatggc atatgaagca ttacggcgag tcgcatgaag atggacgtgc
     9361 ctttgtcgcc gacgggatgg aggtgaagaa attcaacgaa gtccccatga gcgcgatgtg
     9421 gacccagtcg ccgggcgtga atcacgagca gttcggatac aacgcggatg atcgcgagtc
     9481 ggcatcggtc gcgcatatct atggacagaa cctcgcagca gcggaatcga tgacggccgc
     9541 ggccgcgcca tgggcgtggt ctccggcgac cctgaaaccc acagcggacc aggaatttct
     9601 caacggcatc aaccgctttg tcatccacga gtcggcgcat cagcccctcg tggacaagaa
     9661 gccgggtctg acgctgggac cctttggcca atggttcaat cgcaacgaga catgggccga
     9721 tgaggcgggc gcgtgggtga actatctggg ccgcaccagc taccttttgc aggaaggcca
     9781 cttcggcgcg gatgtgactt atttctacgg tgaggattcc aacctgacgg cgatctttca
     9841 tgacaaagca ccggatatgc cgccaggcta cgggttcgac tacgtcaacg ccgatgcgct
     9901 gatccatgtg ctgaacgtgg cggatggacg catcacgaca ccgggaggaa cgacctaccg
     9961 gctcctggtg ctcgatccgt acagcaaaca catgtctctg cccgtgctcc gggccatcga
    10021 caaactggtg cgcgagggag catcggtcgc cgggccgaag ccaacggatg atccgagcct
    10081 cgccgacgat caggcagagt tccacaggct gaacgacgca ctgtttggag atggcaccgg
    10141 gacccattca gtcggaaaag gcaccgttta tgcaggcgac gatgccaccg ccgcgctgaa
    10201 agcaatgaat gttccaccgg actttcacta cacgggaagc aagaaccgcc tcgagttcgt
    10261 tcatcgcagg ctggcgagtg gcgatctgta tttcgttgac aaccgcagcg atgacagcgc
    10321 agccgtccag gcgactttcc gtgtcaccgg caaggcaccg gagctgtggt acgcggagac
    10381 aggccagacc aaacctgtat cgttcacgat tgccgatggg cgcacaacag tgccgctgga
    10441 gctcgaacca tggggcacgg tattcgtagt tttccgcaaa gcgacaagcg agacgtcgca
    10501 tactctgccg gctgtcacgg aaaagcagat ggcgacggtc tcagggccgt ggtccttgag
    10561 cttcccgccc aactggggag ccccgccatc cgtcacactg gacacgctgg catcgtggag
    10621 cgaaaacagc gacgcaggcg tgaagtattt ttcgggtacg ggtacgtaca cgaaaaccat
    10681 cgatgcaccg gcgagctggt tcagcaaggg cgcgcatgtg tggatcgacc ttggcgacgt
    10741 aaagaacctt gctgaggtta tggtcaatgg caagtccctg gggatcgtct ggcatgcgcc
    10801 gtatcgcgtg gacgcaacga ccgcgctgaa gccgggcagt aatcggctga cggtcaaagt
    10861 cactaatgcc tgggtgaatc ggatgatcgg agatcagcaa ccggacgcag caaagaagta
    10921 cacgttcgcc gatgtgaaac cttacaaggc gacttcgccg ctgttgccct ccggactgct
    10981 gggaccggtg acgatcgtgg aggaaagcgc ggcccgatag agcgcgggca ttgactctgg
    11041 aacccgctgt gccgttgcga tggcggcgca gcgaggcgtg aagcacctgt aagggaaggc
    11101 gtgcatgacc aaatccagaa agatcgctag tcccttcagc cctcaagatc caaagcgatc
    11161 acttcgtgca gttcgatact tgggacttca atcacaatcg caccggaatc ttcctgaaaa
    11221 gagatttcgc cgccggcgac tagcctggat gccttcgtca cgcgtcttcc atcgttggga
    11281 atccggatcc gcaccacctg gcgcgagatc ggaatcacct cacgcaccgg tcccttcatc
    11341 atcatcggat tcgtaagatt cacaaggtga gccgtgactg agcctttttg gctccagatt
    11401 gcaacgtcaa gaattccttt gccttcgacc tcgagtgggg caggttcatg ggtagcccac
    11461 agcaccgcat tgcgcagcag cttcgagtga tctgtcgcca gcacatccca gaaggtgcga
    11521 tcgatgtcca ctggaaaata aacagaacgg ccgcggccga tctcgcgcaa aaacactccg
    11581 ggggcacgat ccccggtggc acgcggaaag acttcctcca tcggcaaatc gggatagggc
    11641 ggaaccacct ccaggggaga gtagctgtct gggtcgtgtg tctccacgtg aacccaatgc
    11701 gttccgttaa taatccgccc cgcatcctcc agtcccgcca gcagcgcatg atactcgcct
    11761 gtcttcggat ctcctttgag actgaggtag gaattcagca tgggaccttc cactttgccc
    11821 gcgaaggaag cgccgaataa atcggctagg ccgaaatcgc tgcgccttcc gccgcgctcg
    11881 tcataaagcg acgtttcgga cgtggcaaca atgccgccgc ctttgcgaac aaacgccctg
    11941 atctggtcgc actgccgatt ggagagcaca gcgatgttgg gcagaatcag ggttcggaac
    12001 tgaccaagat tctcctcgtc gagcagtccg tcgtgcacca tctcaaatgg gatacgagct
    12061 tcaatcagtg cctcgtaaaa gccaagctcc ggatcctcaa cgcgcgatgt tgcatccggc
    12121 cagccataag atgcggccgt ttgctgcgag tacacaaggc caacacgagc cagagacctt
    12181 tcgttccgga gatatcgctc attggcaaaa tgccactgga atacctgctc gacaatcgga
    12241 aaccagcgtt tgtcgaacgg cttggcattg aacttggtga atgtgggccg gaagttatgc
    12301 gcaatgccgt ccaccatcca caagcggatt tcttcgccgt cctgcaccga atccttccag
    12361 cgattcctgt cgtcgagtcc cacgctggtc aaacctgcaa cgggcttcat tcccatggtg
    12421 gatcgatatt ctttcgcgga ttttccggcc atccacggag ccatcagccc gcggcgcgcc
    12481 tgacgatctg caatggcggc cggtgaaatc tcggcgaacg tcttcatgtc gagctcgctc
    12541 agagctccgc cgccggcgtt cggcaaataa catgcgttcg gatgaatagc ctgaatcgtc
    12601 gagttccaca ggcggcataa gtcgaacaat cgctcctgac gccagaccat gtactgccgg
    12661 cgagccgggt cgtggggatc gcgggttcgc ggcagttcaa gccctgagaa ttgatgaaag
    12721 ttcgcccgac agtgctcgca gtaacacatg cctgatcccg cccatcgatt ggtgaagatg
    12781 gcatcgggtt tgtacaagcc catgatttcc tggtgcacgg cggtcataaa ttcgaagttg
    12841 tagggcccga gcgcgcacgt cagccagaaa tccttgtcgg acggatgccg tacctgattg
    12901 ccgtgttcgt cgaccatgag ccagtctgga tgcgcatcgt aaacatcctg atgacaagcg
    12961 tgtgcgtcgg ttctggcaac tacgttcatt cccatactgc ggcatcctgc gctgatctct
    13021 ccaaaggcgt ctctgtcccc cagccattgg ctgcggtagt gcagcgggat ctttgtcggg
    13081 taaaacgcga cgcatccccc agcgtcaagc agcgcggcgt cggcatggat ccgcctgaag
    13141 tagtcgagcc agaacgccag atcgtaatgc cccggatcgt cttcgacaaa actcagttga
    13201 gcccagcgca tggggcggtc gtaccaactc cccgcctggg aatttttcga actgcttctc
    13261 aactcggccg ccgctgcgtt ccccgccgcg tcgtcaaagc cgacaaggca cgcggcagaa
    13321 agaccggcgg cgtgtttcag gaatgttctt cttgaactct gcatggaaag gctccgggga
    13381 taaaggcgga gtgtacaccc aatctgctca tgattgaggt gccggccttg acggaatggg
    13441 tcgctggaac ccaacatcgg gacacgatga atccggagac caggaatgtt tgaaacggcc
    13501 tcggcttggc accaaacgta ttctctcttt ggcagcagtc tgggtgtatc tgcgttgatc
    13561 gcggcggtgc caatttttgt atttctcctg cttcttggcg tattgagaaa gcccgcgtgg
    13621 atggccggac tcgttggtct cggcgccgcc ttcgcattgg ccgtcggcgg ctacggaatg
    13681 cccctcatcc ccgcggcgag cgcggcgctc tacggcgctg cgtttggtct gtttcccatc
    13741 tcatggatca tcttctgggc cattgcgctt tttcgcgtga cctccgaaag cggcaagttc
    13801 gaaatcatcc gcggctcgat cgggcgcctt acgcccgatc cccgcctgca ggctttattg
    13861 attgcctttg ccttcggagc atttcttgaa ggtgccgccg gctttggaac gcccgtggcc
    13921 atcgctgcca ccatgctgag cggtctcggg ttttccgcgt tcagcgcatc cgccctttgc
    13981 ctccttgcga atacggctcc ggttgcgttt ggcgccatcg gaattcccgt catcactctg
    14041 gctggcatca cgggtctgcc gctggacaag ctcagcagcg cggtcggcat gctgtgctcg
    14101 cccgtagccc tttttattcc tctctatctg attgtggcca tggctgggtt tcgcgcactc
    14161 tcgggaatcg tcctgcccac gctggtagcc ggcgcttcgt tcgcgattgt ccagtatctg
    14221 gtgtcgacat tcatgggacc gcagctttcg gccctgcttg ctgcgatcgt ttccatgctt
    14281 tcgctgatcc tgatcgtgcg cctccgccgt cgtcgcctga aagacaggga taccacgttc
    14341 tcggaagtgc cgggagccga tctgcgtacc gtcggcacgg atggatcgtc agcccctccg
    14401 caaaaagcca gcggcctggc taaggagcac agcgcacgtg aagtagcggt tgcctggatg
    14461 ccctacgtct ttcttgttgg ctgcgtgatc ctctggggtg tggcgcccat tcatcaggct
    14521 ctgaatcggg caacactgaa cttcaattgg cccgggctgc acgaccttgt gctgcgcatg
    14581 ccgccggtca gcaaagcgcc ttctccctac cacgctccgt tttcgctcaa tttcatctcc
    14641 gccgccggca ccgcctgcat ggttgcaacg ttcctctctg cgttctatct gcggatcggc
    14701 ctaccagggc tggtgggcgc tctgacgcgt accaccaaac agctggctgt gccaatcgca
    14761 accgtagccc tggtgcttgc tgtgggattt cttatgaact actgcggcgc aaccgcgacc
    14821 ctcggtcttg cattcgcctc caccggcagg ttgtttccct tctttagccc tctgctcggc
    14881 gtgctgggag ttttcctcac cggctccgat acttcatcga acgccctctt cggaaatttg
    14941 caggtggtca ctgccagtcg actcggactg gatccggtcc tcatggccgc cgccaatgcc
    15001 tccggaggcg tgatgggcaa aatgatcagc ctgcaaagta tcgccgtcgc ggcggcagcc
    15061 acgcggctat cgaatcacga tcagggccgc ctgtttcgat tcacgctgaa gcatagtctg
    15121 tttctcgcat tcgtcattgg gtgtctggcg atgctctacg cctatcgcct gcatctgtag
    15181 acccaagctt cgtgatctct cccgtcttta tatcgatact ccacggccgc ggttccggaa
    15241 gatgcagagt gccgtctccc atctccagcg gcatccagat gtaccgcgaa tcccaaagct
    15301 tctgtgggtt ccagcggtcc cccatgtaaa tcacggtctc tgtttctttc ccgtccactt
    15361 tgatcagcat gcttgactgc gaatcgtacg tattggtctc cggaggtgcg atgtctttca
    15421 tcgcagtcca cggccccgcc agtgttggcg ccactgcgta cacattcgga ttcggatgcc
    15481 atcccgtcag gtgcgacccc agcacgtaat aaaacccttt gtaatggacc agcgcaccgc
    15541 cttccagcgg cacaggaatg aagctgacct ctttctcaac gttcaggtaa tcgtcggaga
    15601 gcttcgcgat gaagaatcca tgcgtcggcc gcgattcgaa gatcaggtac gccgagccat
    15661 cgtcgtccac aaattgccca atatcgcggc tctcctgccc cagcggacga aagctcctca
    15721 gataccggta cgggccgtcg actttgtcgc tgacggcaac tccaacccgc gccagcttgt
    15781 atttggagtc gtcgaagtgc atgtacatca cgtacctctt cgtgcgctgg ttgtagaaca
    15841 ccttcggccg ctccagcaca aacgggctgc cggggtcctc cgggttcgcg agcttgaaca
    15901 cctgcccgcg aaaacgccag tgcgcgagat ccgttgacga gtaacaggtc acgtatccca
    15961 tgcccggatc attgtcctgc gaccgatctt cgccaaacca gtagtaggtc ttgccaagat
    16021 gcaaaatccc gcccccatgc gcctggatct ccctgccacg gttatcgagc cagatcgagc
    16081 ccggatcgat gactccggaa cgatgcggtg ccgccccggc aagagctgct gttgcaacca
    16141 ggacgttcac aatcaggaat cgtccaacgc acttaggcac ctgcgcctcc cggactcgtt
    16201 tcatagccgg ctaccctttc gtcgcgaaac gtgcgagccg ctgttctgtg cctctgtctt
    16261 atcgaatgcc ggggctgcac ttctcagcaa ctccgccgct tcgactcccc gcatcgacgc
    16321 atccaccaac tccaccaggt gaaatgcagg catggctctc tctccacgcc gcctcaggcc
    16381 gctcatcaac tgcaacaggc accccggatt ggccgatacc accgcttccg cctgcgtctc
    16441 aagcagattc gcgaccttcc gatcccccag ttgctcagcc gcctcaggct gcagcacgtt
    16501 gtacacgccg gctgagccac agcaaagcaa agactcagca atctcggcga tttccagccc
    16561 cggtacggcg ctcagaatct gccgcggctc acgataaatt ccctgcgcat gtcgcagatg
    16621 acaagcatcg tggtacgcca ctcgcagttt cagcgggttc ctcgtcgcga tcggctccat
    16681 ctcgcacagt acctccgcaa tgtctctgca ttttgctgaa aatgcccgcg cacgctccgc
    16741 ccacaccaaa tcgtctcgca gcagatatcc atactccttc atcgtcgaac cgcaaccggc
    16801 agcgttgata acgatcgtat cgacctctgc ggactcaaac gcagctatca tcttcttcgc
    16861 catcgccgcc gcatcgcgct tcaggcccgc atgcagcatc agcgctccgc agcactcctg
    16921 ctctgacggc acaatgacct cgcaaccttc tgccgacagc acccgcaccg tcgccgcgtt
    16981 cacctgctgg aagaacacct gctgcacgca tccggccagc atcccgaccc gccgtcgcgg
    17041 cttgctaacg gccggggtcc ggaccggcat cgtcgccctg tattcggccg gcaccgtcgg
    17101 cagcagcgct tccatcgccg ccaccttcct cggcagcagg ttcagcagcc ccatcctgtg
    17161 caaaacccgc tgcagccccg aacgttgata cagccgtacg gcccccgcga acacgcgtag
    17221 acgcgacgga taaggaaatg tcgcaaacaa cagtcgccgg aaccaggagt cgctcgccgc
    17281 gcgcggaatg tttcgctcca cctgcgctcg cgtgtcttcg atcagcttgc cgtactgaac
    17341 ccccgacgga catgcggtca tgcatgccat gcatccgagg caccggtcca tgtgcgcctt
    17401 aaacatctcg tccagctgcg ccgccccatc cgccgccttc tgcatcatgt aaatccggcc
    17461 gcgcggcgaa tccatctcct cgccccacag tacgtaggtc gggcaggcag gcaaacagaa
    17521 accgcaatgc acgcaatctg ccagcagatc ccggtccggc gggtgatccc cgtcgaagct
    17581 cgatcgcttg ggctccgcga gattcacaag cccgctcatc cgccgatcac cacacccgga
    17641 ttcaacagac ccttcccatc aaactgccgc ttgatctctc gcatcagccg ccccgcgtcg
    17701 cccccaggcg gcagcacacc atatccatcc tctccgcgga gcaccgtcac cgcacccacg
    17761 ccctcggctg caaccaactc gcgcagccgg tcgaccgtac tcgcgtccgc aaggcgcgcc
    17821 accatgatcc ccgaagcctg ggtaacggcc tctccgccca gccggaccac ctccgccgac
    17881 atccgggcaa tcgccgatgc gagcattgtc actttcgcca cgactccgtt acctgtaaac
    17941 agccgctctc gggcggcgaa aacatcacca ggaattgcgc ctgcgcaacc agccaacgaa
    18001 tcgagtaaac tccactgctc ccgcatcact tcgtcgattg acgcaatcgc cacatccagc
    18061 gcaaatcccg cagcattcgc ccgcaactgc atcgcctgca ccctcaactg tgaatccagc
    18121 accttgatca gcatctggcc gcacgccact gcatccgtcg actcgaagct ccaggtccga
    18181 ctattccgcg gaatcgaatg cagccgaaac gtcgcatccg tcacaaccgc cagcgtgccg
    18241 aaggcaccga tcatcagctt gtgcagatcg tatccagcaa cgttcttgac cactttgccg
    18301 cccgatcgcg cgatcgttcc atcagccagc acgatcgtca tcccgatcac caggtcgcgc
    18361 aggctcccat acctgaggcg aaacgcaccc gaatcgttcg ccgcaatcac gccgccgacg
    18421 gtcgctcgtt ccggccagag cggatcgagc gcaaccatct gcccatgctc tgcgagcacg
    18481 cgctgcatct ccatccaggt cgtaccggcg ccgacggtcg ccgtcatatc ctgccagctg
    18541 tgctcgcgaa cgcctgcaat cccgcgggtt tcgagcacta cgtccccgcg cactgcaccc
    18601 gcccagcggc gcttcgtgcc cgcacccaca atctccacgc cgaatccgtt tgcctcggca
    18661 ttctgcagaa ccatcgcaac ttcctctgtg ctccgcggcg aagcaacaac acggccatcg
    18721 cgctcgctca caatctcggc gccaatcgtg cgggcaaact cctcgaagcg gctggtcagg
    18781 tgctccatca gaaaaactcc gccacaccag cccgttcgag cggatgcggc gtgtacggtc
    18841 cggtcttctc cccgcatagt cgagtccgcg gaaacacctt gtcgggattt gagaggttct
    18901 gtggatcgaa ggctatacgc acccgctgca tcgtcgccag atccggctca gaaaacatgt
    18961 agcccatcgc ctcctgcttc tcgcgtccaa caccgtgctc gcccgtgatg gagccgccgc
    19021 gatccacaca cgcacgcagg atgcgatacg acaagtccag ggcaatctcc tcctgccccg
    19081 ccacagcctt gtcgtacaac accaatggat gcaggttgcc gtcccccgca tggaagacat
    19141 tcgcgatgcg cagattcacg gcatcggcca tgcgggagat gtcttcgagt acgtcgggga
    19201 gcgcagttcg cggaatcaca ccatcctgca cgatgtagtt cggcgatatg cgtccgaccg
    19261 ctgcaaatgc ggccttgcgc cccttccaga ccagcgctcg ttcatcagcc gtctgcgcgc
    19321 gcctcacctc ccgcgcgccg ttgttctcgc acagcgacgt cgccgcggca atctgcgccg
    19381 tgacctcagc cgccggccca tcgagttcaa ccagcagcag tgcctcgcac accggatagt
    19441 tcggatggac cgcagcttcc gacgccttca ccgagagccg atccataatc tcgagcgccg
    19501 ccggcaacat gccctccgca ataatgtccg aaaccgtctg cccggccgct cgaatggagt
    19561 caaatgccgc aagcagtacc tggacggtct ccggccgttt cacgatccgc agcgtcagct
    19621 tcgttgccac gccaagcgtg ccctctgagc cgacgaagag accggtcagg tcgtagccgg
    19681 gggggtcggg cgtcgcagac ccgaagtgca ccaattcgcc atctggcaga acaacctcca
    19741 tgcccactac gtgggtcgtc gtgaatccgt acttcagaca gtgcgcaccg ccggagttct
    19801 ctgccacgtt gccaccaatc gtgcacaccg attgcgacga cggatcgggc gcatagaagt
    19861 atccatacgg cgcaaccgcc tccgtcacat ggaggttgat cactcccggc tcaaccacga
    19921 cgcgctggtt tggaatgtcg atgctgagaa tgcggttcat ccgcgcaaag ctgatcacca
    19981 cactgtcttc ggcgggcagc gccccgccgc tcaggccggt tccggagccc cgcgccacaa
    20041 acggaatccg gtgccgggca cagatgcgta cgaccgactg cacctgctcc gccgactgcg
    20101 gcaacacgac cgctccgggc atcgtgcgaa agttggtgag cccatcgcac tcgtaggtct
    20161 gtaattcgct cgcagcggtg atcaggccgc cgggaacggc tgcgcgaagt tcgtcgatga
    20221 tgctgtcacg gagcacgggt cggttaccca tcttacccgc agggaagggc gtccggcatc
    20281 gaccggcatg gagggatcag gtgcggacat cgtcgtcgca cacaggatcg gactcccgcg
    20341 ttcttcagga tggaagctcg gctgagtccc ctggtgttgc agaatgttcg aggacaacga
    20401 gagctactgc gatgcagcct tcgccttgtt ccttggctgc tttttggctg gtttcctgcc
    20461 gcgcagcagc ggccggcgct cccgcacgat gcgatcctgc agcagaagct ggtgctgatg
    20521 ctcctgctca tgagtcagga tagccgttgc atcagtaaga tgttcggcca tcagccgggc
    20581 cgctcggttc ccatcgcggc gttcaatcgc ctgcatgatt ggcttgtgaa aagctagcgt
    20641 gtgttcgagg tcgaccagct gcgaggtcac cagaatcatc ttcgccatgg cccgatgaat
    20701 gctgtgaaag acgctcgtcg cggtgccgtt cttcgaagcc tgaaaaattg cgcggtggaa
    20761 cagaagatcg gacgacacga tcttcagatt gtcgcccccg ctcgactcga aatcgtggat
    20821 ggactgccgc agcagcgcaa tatcttcggc cgtggcccgt tctgccgcca gacgcgcaag
    20881 tcccggctcg atgagcagcc gcagttccgt caactcgccc accgatgtgt catcgagcag
    20941 aaaaaggaat tccatcggca cagccaggat ggcagagctg tcggtattga gatagctgcc
    21001 atctccaact ttctgcgtga tcacgcccat cacctccagc accttgagcg cctgacgcag
    21061 tgaggaacgg gcgacattga agtgcgcagc cagatcgcgc tccggaggaa gacggcttcc
    21121 ctggcgcagg agcccatcgc gcagcatttc ctggaacctc ttcaccagta gagaggtgac
    21181 atcttctttg ccattgccct caaggaaaga ggtggatttg tacgaccgat gggacagctt
    21241 gcgcatagca gcatcatcat tcacagcgcg tggcgcgtca acgaatggcc tgcggcttac
    21301 ctttgcacgc caacctcgcc tgatactcgc agaacaggaa tagtagttca catcttgaca
    21361 actgtttcgt aatggtcgta cctttcagtg cattcctgag agccccttcc ccagatcggg
    21421 caccagcaac ccggaggcaa acaagatgtc gtcatactgc gtcgtatgcg gcaggtccgc
    21481 gcgtgtggta gtcgttcttc tttttctggc gctaagcctg atcgcgcacg gccaggtgga
    21541 acagggccag ttcgtgggga gaattaccga tccttccggc gccgccattg ctggagcaca
    21601 actggaagcg cgcaacgtcg agaccaacat cacctacaag gcggtcagca atggcagcgg
    21661 agactacgtg atcacgcccg taccttcggg caattacgtc attaccgtag tcgcgcaggg
    21721 gtttcagcag gcagcgacaa aaccggttga attgcaggtc ggccaggtcg cccgcgttga
    21781 tcttccgctc accattggta cgaccaccac cgttgtcaat gtcaatacct cggcgcctct
    21841 gctctcaacc gattcggcca ccattcacca ggtgatcagc aaccgtcagg ttctcgatct
    21901 gccgctcaat ggccgcggct acttccaact ggcggaactg acgcccggta ccgctttgct
    21961 cgctccaacc ggcaactccc tggccatccg tccggaggtg gtgaacggca acgtgatcag
    22021 cggtatccgg gggagagcca cctcgttcct gatggacggc gtcgatgtga ccgagcagca
    22081 tcagggcggc acgttcatcc agacctccat cgatgccctc caggaattct cggtcgagca
    22141 aagcccgtat tctgccgaat acaaccgcgg cggcgccttc tttaacgcca ccatcaagtc
    22201 cggaaccaac tcctggcacg gaggagtctt cgacttcctg cgcaacgacg cttttgacgc
    22261 ccgcaacttt ttcgcgctgc agcggcagcg gctcaagaga aatcaattcg gcggtgacgt
    22321 gggcggcccg gtttccattc ctcacctcta caagggaaaa gaccgcacct tcttcttttt
    22381 cagctatgaa ggccagcggc tcagtgaagg cctggtgttc aacgggatcg tgccttccga
    22441 tgctcagcgg ggcggcaact tcgggtccaa gccgatctac gatccggaga gcagcgtgac
    22501 gaccggcaac gtgaccacgc ggcagcagtt cacagacaac atcatccctc aggaccggat
    22561 ttcgcagcgc gcgctcgcta tcctgggtta ctatcccaaa caaaacacca ccaccggcac
    22621 cttcagcgcg gtacccgcac aaaccatcaa tttcgaccag tacatcatcc gcctcgacga
    22681 tcagttgacc accaacaatc gcctcttcgc gcactggatt tacatgacgc agcacgaggt
    22741 ggatccgaac ttctcgcccg cgttgggccg cgcgaatctc acatcgatcg ggcaggatat
    22801 tgctgtgggc ctgaccacca gtcttggctc aaccatgatc aatcaggcca gagcgcatta
    22861 cctgcccagt catgtgcgtc tgcaggcttt tctccagggc aatgatttca acggcgactt
    22921 cggcgtcgga ggcttttccg gccttcttag gaatggcaca tcctccttcc cggattactc
    22981 ctggaccggc tatgcgtccc tgcagggctc gaccttcgat cagcggccga agtcccagga
    23041 tcgcaaagcg gtcgagttct ctgacaactt cacgaaaatt ctgggtcgtc agtccctcaa
    23101 ggccggcatt ctggtccgct actaccagtg gcttggctac gacagcggaa cttatgcggg
    23161 cctgttcagt ttcaacggaa acgaaacagc gcaggtgacg gtgacgcaga attccgacgg
    23221 gacgaagaca acgaccgcat cgggcggcga cgcatttgcc gacttcctgc tcggctatcc
    23281 ctcggctgtg cagcgctcgt atccgggata taactttggt ggacagggat ggtcgaagca
    23341 gttttacgtg caggatgata tccggatcag cgatcgcgtc acgttgaatg ctgggctgcg
    23401 ctacgaatac acgccatggc tgaatggcta tcgcggtcag gtcggtacct tcgatcccac
    23461 gctgtccaaa ccgatcatcg acagtggaaa aggatcgacg ctggatcttt ctgcccagcc
    23521 tggagcaccg gccgcctacc agtattttgg ccaatatatt cagaccagca gcgaagccgg
    23581 cctgccatcg aatatcagct acaacgatac gcggcagttc ggtccgcgcg tgggtgtcac
    23641 ggtgtcactc gatccgaaaa cggtcgttcg cgccgggttc ggcattttct atgaacccga
    23701 aaatacggat ggccgcctga acctgaatat gctgcccttc cggctgaacg aaacccagaa
    23761 ccagacgcag aatacgaccc cgacccgcag cctttcagac ttcttcctgg gtgctcctct
    23821 cggatccgct ctggccaatc cttccctggt gccgaccaaa gtccatatgt cgatgggccg
    23881 gaatgctcac tacagcctcg atgtgcagcg gcggttctcc gatcacgaca tgctcgatgt
    23941 gggctacgtc ggcaatcgca gcctgcatct gaatgcgacc aatgacttca acgatccggc
    24001 cccaggccct ggctcggtgc agtcgcggcg accctaccag ccatggtccg gcatcacctt
    24061 cgacacccag gacatgtcca gcaactacaa tgcgcttcag gcgaaatacg aacatcgctt
    24121 cagtcacggg ttcaatctgt tggcctccta ttcctggtca aagtggctgc agttcaatca
    24181 ggcgccggcc ctggggggca atacgggtta cgaatacgct cgagccgcct cggatatccc
    24241 ccaaaacctc gctgtgagcg ggatctacct ggtgcctttt ggccgcggcc gccatttcct
    24301 caccaacggc aacggcctcg tcgacggtat cctcggcggg tggcagcttc aggcgatcgt
    24361 cgaccagcgc agcggcattc catttacgcc gatcgtctcc ggagaccgcg cgaacaccgg
    24421 cgtcggatcg cagcgtccca acctcaaccc cgcaggctgc agtgcaggat tccatcgttc
    24481 gctgaccacc tggttcaaca agtcatgcta tgtcgatgga cccgtctata cctatggcca
    24541 ggttcatgcc aacacgctcc gtgcggatgt gtaccggcag tacgacgctt ccatctttaa
    24601 gaatttcagc ctgccgcgcg agagcactct gtcgctccgg gctgaattct tcaacgtatc
    24661 caacaccacc tcgttcaatt ctccgaatgc cacggtcgat gtcgcggccg gtggcagggt
    24721 gacggcaacc tcgaaccagc cccgtgagat ccagctggcc ctgaagtaca gcttctaaca
    24781 ttgcgtgaac aggctcatca tcgacacgtg gggtccaaac ggaaagaagg ggagaatgca
    24841 ggcaattgtt cgcaggctaa tgccgttatc cgctctactg actttggtgg tcccgttggc
    24901 gcagggtcag ggagtcattc cccctccgcg accagcgccg gatcagccaa ccgtcagcat
    24961 caatgtcgat ctgtcgcaga agatcgcccc gtaccagccc atttacagct ggttcggtta
    25021 tgacgaggcg aattacacca ctacgaatct cggccgggct ctgacgcatg aactggccga
    25081 tctgagcccc gtgcccgtgc acattcgcgt gcatcacctg ctgacatccg gcaatgggga
    25141 gcccgaactg aagttcagtt cgaccaatgt ctatcacgag gatgcatccg gtaatcctgt
    25201 gtacgacttc accatcctcg acaacatctt cgacgcgtat aaagctgccg gtgtcgagcc
    25261 gatggtcgag ctcggtttca tgccgaaaga tcttgccgcg gttcttccca accgaaatga
    25321 accgtatcag gtccactatc cgcacagcac cgtcagcggg gcttccaaca atcccccgaa
    25381 ggactacaaa aaatggggcg atctggcaaa ggccgttact gcgcatctcg tcgagcgcta
    25441 tggccgtgaa caggtgcaga agtgggattt cgaagtctgg aacgaaccgg atatcgatta
    25501 ctggcacgga tcgcgcgagg attactttcg cctctatgac tacgccgtcg ctggcgtgcg
    25561 agcggccttc cccgcggctc gtgttggagg ccctgcaacg accggcccct ccagccagcg
    25621 ggctgcagac tttctgaagg cctttctcga ccacgtcgca aacgacaaaa gtgctgccac
    25681 cggcggcgcc atcccgctcg atttcatctc ctttcacgtc aagggacatc ccaccatagc
    25741 cggaggcgac gtgaccatgg ggctcaacca cgagctgtcc gacgccgatc acggattctc
    25801 gatcattgcc cagtacccgg ccttccgcaa actgcccgtc atcctcagcg aggcggatcc
    25861 cgagggctgc gctgcctgct cctctcgcgt caatccggcg aataactaca ggaacgggac
    25921 cctctacccc gcctacacgg caaccgcctt caagggcctg ctcgatctcg cgcagcaaca
    25981 taaaaccaat ctgatttcca tgctgagctg gtccttcgag ttcgagggca aggattactt
    26041 tgaagggttc cgctcgctct cgacgaacgg catcgacaag ccgattctca acctcttccg
    26101 catgctgggc atgatgagcg gtacgcgagt gacggcgtcg agcagcggca gcgtgcccct
    26161 gcagacccag gtggagacgg gcgtccgcgc ttcagccgat gtcgatgcgc tggcaacccg
    26221 gcacgatcga ggggcagagg tgctggtgtg gaactaccac gatgaggatc aaccggcacc
    26281 ggacacgccc acccagctga ccatccgagg cattcctccc ggcgttcaac gcgttctcct
    26341 gcagaattac cgcatcgacg ccacccacag caacgcctat accatctgga agcaaatggg
    26401 attgcctcag cagccgacga ccgaacagta cgcgaagatc agagccgagg aagggctgca
    26461 gttgctcgat tctccccaat ggctcaatgt gagcgacgga gcggttcgca tctccaccct
    26521 gatgcctcgg cagagcgtta gcctgctggc gctgagctgg tagagaggaa ctcgccgctg
    26581 agatggctcg caaatggtac ttcgtcttcc ttgtttcgat cgcgattgcg atcagctact
    26641 tcgaccggct gacgctgccg gtggctattg ctgccatcca gcacaatatc cctctctcga
    26701 atgagcaata ctcctggctc cagactgcct ttttgctttc ctacgccgcg ttgtatgcca
    26761 tcggcggcag gctgctggac tggctcggta cccgcagagg ctttctcctg atcatgctgt
    26821 ggtggtcgct ggcgtgtgcg ttgcacagcc tggccagtgg attctttttg ctggtcgttg
    26881 cccgctttct tctcgggatg ggagaaggcg gagcgtttcc ggcggccacg cgcgttgttg
    26941 ccgaatggat tgacgcacca gagcgctcca ccgccatggg cctgatcaat gccggaaccg
    27001 ccatcggatc gctgctggct ccgccgatca tcggtttcat tctgctccgc agcggatggc
    27061 gcgccgtttt catcgcggcc ggcgccgctg gtctcgcatg ggggttttgg tgggcgctct
    27121 cctatcgtgt cggcgccccg ggtgccgtgg ggtctggcga tcccgggacg cctgctggcg
    27181 aggtgaggtg gctcgatgtc ctgcgtctgc cgaacgtaca ggcattcgtc ttcgcgaaat
    27241 tcatgagcga ttcggcctgg tatttccttc ttttctggct gccgaaatat ctctacgacg
    27301 cccgtagctt cgatattcgc caggtcagct actacgcgtg gataccctac gccgcctcgg
    27361 gagtgggcag ctttctcggc ggatggttct cctcgacact gctgcaccgc ggaagctctc
    27421 tcgacgcggc gcgcaagatc gcattgggat gctgcgccgc cgccatgccc gtcgttatgc
    27481 tggtctcact cgtgcccgtc tccatggctc taaccctttt cagcatcgcc ttcttcgccc
    27541 agcaggcatg gtccgggctg gtgatgaccc tccccgcgga tatcttcccc ctgaatatgg
    27601 ttggcaccgt tgccgggctg atcggcttcg ggggagccat gggcggcgcc atcttcgggg
    27661 tcgttgctgg attcctgctc ggacatggaa cctcctacgg cgtattgttc gttgcagttg
    27721 gcacctttca cctcatcggc tttctcgcaa tctttttgcg aggcggcagg attcaacctc
    27781 ttcgcgcgat ggatatcaag tccatcggga gcgctgcatg aaaatcacgg aagtccgctc
    27841 gcgtgtcgtc cagtgggagg gtcctgttgt tcctcttcct ccgcactttt gtacgaaccc
    27901 catggacctg gtttcgttcc gtcaggcctc gatgggaaat tttgcctttc ataactggct
    27961 gctcgtcgaa atcttcactg acacaggact ggtcggtctc ggcaacgccg cgttatcccc
    28021 cttggtcacg aagcaactac tcgacattta cctcaagccg ctgctgatcg gcgccgaccc
    28081 gtgggatgtc gagtacctgt ggcagcagat gtaccggcgc acgctggcgt ttggacgcaa
    28141 aggggtcgcg atggccgcca tttccgctgt cgacatcgcc ctctgggacc tgctcggcaa
    28201 agatgcgaaa cagcccgtct ttcgcctcct cggcggccgg accaaggaca agatcccggt
    28261 gtacgcgagc cgtctctacg ccatgcccct cgacgagctt cgtgccgaag cgcagaagta
    28321 taaggatcag ggctacacga tgatgaaact gcgtttcggc tacggtccgc tcgacggcgc
    28381 cgtcggcatg cagaaaaaca ttgaattggt ccgcaccgtt cgcgacgtga tcggcgaagg
    28441 catcgatctg atgaccgacg cctacatggg ctggaccctc gactatgcca agcgcatgct
    28501 gccgcggctc gagccgttta atct
//
LOCUS       DASZYN010000285         2729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1166771, whole genome shotgun sequence.
ACCESSION   DASZYN010000285 DASZYN010000000
VERSION     DASZYN010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166771"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VHE33_07380"
     CDS             <1..217
                     /locus_tag="VHE33_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97315.1"
                     /translation="NKIAAVENVVEDTDVAPTAQSYEVYDQLSGQLQTQLDQLHKLET
                     SGIADFNKLVREQNVPAVVIPEKKKAE"
     gene            complement(687..1349)
                     /locus_tag="VHE33_07385"
     CDS             complement(687..1349)
                     /locus_tag="VHE33_07385"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HVT97316.1"
                     /translation="MGTVIPLSDASRRPRSWPGATVLIIVLNVFVFFQELVGGNAFVA
                     QWAAVPAAISSGHNWVTIFTAMFMHGGWMHIIGNMVFLWAFGPEMEDVMGHFRYVVFY
                     LLGGVIAMLAQVAAAPLSMVPCIGASGAIAAVMGAFIVTYPGDRIKSLLIILIFVRVT
                     FIPAALLIGFWFVIQLIHAGAVANVQTGGVAYLAHVGGFVFGAATARLWRRKPAWSTY
                     NR"
     gene            1583..2524
                     /gene="cysD"
                     /locus_tag="VHE33_07390"
     CDS             1583..2524
                     /gene="cysD"
                     /locus_tag="VHE33_07390"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF009214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HVT97317.1"
                     /translation="MATALIDAPVSAPAISPLSHIRQLEAESIHIIREVASEFQKPVM
                     LYSIGKDSSVMLRLAQKAFYPGPIPFPLLHIDTGYKFREMLEFRDRYTAELGLKLIVW
                     RNEEALANGANPVALGTRRCCGYLKTQALLDGLRAHGFDAAFGGARRDEEKSRAKERI
                     FSFRDSAGQWDPKNQRPELWNLFNGNIHPGESIRVFPLSNWTELDVWHYIHLEKIPIV
                     PLYFAKERRMLVRGESLIPLEQDFVHGLPGEEQWVRCRLRSLGCSPCTGAIRSDADTV
                     PRIIAELMAMRNSERANRVIDHDQDGSMEIKKREGYF"
     gene            2524..>2729
                     /locus_tag="VHE33_07395"
     CDS             2524..>2729
                     /locus_tag="VHE33_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97318.1"
                     /translation="MALQHSTWAVGDEFLGGAADDGDREVEELLAAERGKELLRFSTA
                     GSVDDGKSTLIGRLLYDSNNVYDD"
BASE COUNT          543 a          771 c          914 g          501 t
ORIGIN      
        1 caacaagatt gcggcggtgg agaacgtggt ggaggacacg gacgtggctc cgacggcgca
       61 gtcgtatgag gtgtacgacc agttgtcggg gcaattgcaa acgcagctcg atcagctgca
      121 caaactcgag acgagcggga tcgcggactt caacaagctg gtgcgggagc agaatgtgcc
      181 ggcggtcgtg attccggaga agaagaaggc ggagtagttc ctggagcccc acatctgcca
      241 aaaccgggca gatgtggggc tcccggcgcg gggctaaagc ccgattcatt tgggcgtttt
      301 gcggcacgac cccaggtcgt gccctgttac aaaagcggct ggtcaggata ctgtttgggc
      361 cgtgtggcgc aagaacggag cgctagtacc atgaccgcgt gattaagtac gttccgggcc
      421 taaaggctcc gaacccgcgg atcctttttc cccggcctga aggccggggc ttctaccgtc
      481 gtgccgccgg cgcggcaccg cttcgcggga gggacccgat catccggtcg aagccctggt
      541 tcttatgcga aagagaagca ggtccctcca cttcgctgcg cttcggtcgg gatgacaagc
      601 tgaggaagca ggtccctccg cttccccttc gctacgctca gggtccggtc gggatgacaa
      661 cgtaaaggaa cttcagaaac accacactag cggttgtagg tgctccaggc gggttttcgt
      721 ctccacaggc gggcagtcgc tgcgccgaaa acgaatccac cgacgtgcgc cagataggcg
      781 actccgccgg tttgcacgtt ggcgacggcg ccggcgtgga tgagctggat cacaaaccag
      841 aatccgatca gcagggccgc gggaatgaag gtgacgcgga cgaagatcag gatgatgagc
      901 agcgacttga tgcggtcgcc ggggtaggtg acgatgaagg cgcccatgac ggcggcgata
      961 gcgccgctgg ctccgatgca aggcaccatg gagagaggcg cggcagcgac ctgggcgagc
     1021 atggcgatga ctccgccgag caggtagaag acgacgtagc ggaagtggcc catgacgtct
     1081 tccatctcgg ggccgaaggc ccagaggaag accatgttgc cgatgatgtg catccagccg
     1141 ccgtgcatga acatggcggt gaagatggtg acccagttgt ggccgctgga aatggcggcc
     1201 ggcactgcgg cccattgggc gacgaaggca ttgccgccga ccagctcctg gaagaacacg
     1261 aaaacgttga gcacgatgat gagcacggtt gcgcccggcc aggagcgcgg ccggcgcgac
     1321 gcatcggaca atgggatgac ggtccccaca atttctcctc ggcgcgtcgc gtcccgaacg
     1381 atcgggtgat ttgacccgcg atacgcttgc ccctatagta gcttcaatga ggtttggtcc
     1441 cactccggcg actctgtgtg catgttgttg catgctgcgc cggacttgtg ggtaagtaag
     1501 cctcagcgtc tttctaccca taagcccgcg cttcagcctt tgctgacgcg ggctttttct
     1561 tttccccaat ccccggagag ttatggcaac cgcactcatc gacgctcccg tctcggcccc
     1621 ggcgatctcg ccgctgagcc acattcggca actggaagcg gagagcatcc acatcattcg
     1681 cgaggtcgcg tcggaatttc agaagccggt gatgctttac tccatcggca aggattcctc
     1741 ggtgatgctg cgcctggcgc agaaggcgtt ttatcctggg ccaattccct tcccgcttct
     1801 gcacatcgac acgggctaca aatttcgcga gatgctcgag tttcgcgacc gctacacggc
     1861 ggaactgggg ttgaagctga tcgtgtggcg caatgaggaa gccctggcga acggagcgaa
     1921 tccggtggcg ctggggacgc ggcgctgctg cggttatctc aagacgcagg cgctgctcga
     1981 cgggctgcgg gcgcacggtt tcgacgccgc gtttggcggg gcgcggcgcg acgaggaaaa
     2041 atccagggcg aaggaacgca tcttctcgtt ccgcgacagc gcgggccagt gggatccgaa
     2101 gaatcagcgg ccggagttgt ggaatctgtt caacggcaat attcatccgg gcgaaagcat
     2161 ccgcgtgttc ccgctttcga actggaccga gctggacgtt tggcattaca tccacctcga
     2221 gaagatcccg attgtgccgc tgtactttgc caaagaacgt cgcatgctgg tgcgcggaga
     2281 gagcctgatt ccgctggaac aggactttgt tcatggcctg ccgggggagg aacagtgggt
     2341 tcgctgccgg ctgcgctcgc tggggtgcag tccgtgcacg ggcgcgattc ggtccgacgc
     2401 ggacacggtt ccaaggatca tcgcggagct gatggcgatg cggaactccg agcgggccaa
     2461 tcgtgtgatc gaccacgacc aggacgggtc gatggagatc aaaaagcgcg agggctattt
     2521 ctgatggctc tgcagcacag cacatgggcc gtgggggacg agtttttggg cggtgcagcg
     2581 gatgatggag accgcgaggt cgaagagctg ctcgcggccg aacgaggcaa ggaactgctt
     2641 cgcttcagca ctgcgggcag cgtcgacgac ggcaagtcga ccctcattgg gcggctgctc
     2701 tatgactcga acaatgtgta cgacgatca
//
LOCUS       DASZYN010000286         5299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_13097, whole genome shotgun sequence.
ACCESSION   DASZYN010000286 DASZYN010000000
VERSION     DASZYN010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13097"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(512..790)
                     /locus_tag="VHE33_07400"
     CDS             complement(512..790)
                     /locus_tag="VHE33_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97319.1"
                     /translation="MSLKSRIAVLEKKLPPKRAFVGSLRFFATLAEEYQPTPDELGEL
                     AEWDRQAEAEVHAESVEEELARLTAEIRPPCGLRELPALDMMNREHRM"
     gene            complement(787..1140)
                     /locus_tag="VHE33_07405"
     CDS             complement(787..1140)
                     /locus_tag="VHE33_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97320.1"
                     /translation="MSLASGCTIEVAARECGAAVRTVKLWLADVPGFRRRVEGLRSEM
                     TSQALGRLIDNMASASDSLGYLSRKGKSEMVRLSAARAVIELGCKLRETVELAERVAK
                     LEAAQPQPAPKPRIA"
     gene            complement(1644..3560)
                     /locus_tag="VHE33_07410"
     CDS             complement(1644..3560)
                     /locus_tag="VHE33_07410"
                     /inference="COORDINATES: protein motif:HMM:NF024367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3854 domain-containing protein"
                     /protein_id="HVT97321.1"
                     /translation="MSRKEQGEKKTLAVAQTGLLAVGIGGVFCWKVKGSEELIPELLA
                     LARLGGVCHIGFDYEPKPETQRKVAGALRRLARALRKAGVKEVLAVEKPPGPDGCKMG
                     DDDFLAAFGPDAFRELVEKARPVLDRALVVLGTDEFRVNAEAEAALENAEGLYQRGGH
                     LAHVVQHDPSSEKALVRRPSTAPIVRALPQALLREELTKLVDCVRLVKVKEGVEHRPA
                     SLPGHVVATIHCRGAWENVPHLEGVVTHPALLADGSLLREPGYDEKSGLLLWMPDDLG
                     VEVSDQPTRDDAVSAMTLLGDVVTDFPFKAECHKAAWLASVLTPLARYAFQGPSPLIL
                     LDGNAAGVGKGLLADVGFLIVTGREASVMSYTNDKEELRKAITPLAMEGDEMVLLDNL
                     SGFVGNAVLDRALTASWWKDRILGGNNQFNGPLNVTWYATGNNVYLIGDTPRRVMPIR
                     LESPLECPEDRGDFKYPDLRKHVRNNRGELLSAALTILRAYCVADRPDQQLTPWGSFE
                     GWTSLVRNAVVWCGLDDPGDARKELRRDSSPESIALAGLLKGIAYLDTNGAGLTVNDM
                     LLYCHNESDPDILAFREALFVLCPTEGRGQIVNARSLGMKLHHLRGRVIGGMMLERVD
                     AVSAGVRWRVVTQQ"
     gene            complement(3569..4900)
                     /locus_tag="VHE33_07415"
     CDS             complement(3569..4900)
                     /locus_tag="VHE33_07415"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVT97322.1"
                     /translation="MPFSQTTPAPCQWFSRLATALDRRSAVRLALVFVGAMLARGRRT
                     VTSWIRAAGLSSDYKPVYTTVAAAGKRADAIAARLAWEVVKRVAAASGRLIFALDDTP
                     TPRYGPQVQGAGVHHNPAPGPAGSPFVYGHVWVVLGLLVGHPLWGTIALPLLARLYVR
                     RKNLGSIDARHRPPFRTKLELAVELMRWAATWLGFLGQRLWVVADGAYAKAPFLKPMR
                     GLGVTVVSRLRKDSALWGLPRHRPGRRGRPRVYGESRVELAKRAGQRRGWATSTFILY
                     GKETTKRYKTFVATWRPAGGAIRVVVVDEPAGWVAYFCSDPSAGVADILGCVADRFSL
                     ETAFRDCKEVVGAGQQQVRFVWASVGAFHVCLWTFTMTEVWAWDRHEEGLVGHRCASP
                     WDAEPRRPSHADKRRAWRRELLGEEIRAVLRPGVTEREIAAAADRLLDLAA"
     gene            4998..>5299
                     /locus_tag="VHE33_07420"
     CDS             4998..>5299
                     /locus_tag="VHE33_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97323.1"
                     /translation="MRPIDVRNLIRFNTRMGMFICPSNRPNVVAFVHGYQYATATECR
                     FTELLSAHIADRYRVKADALGWPHQIARFAERRSLDWMDAYLLLSSELLNSAIGAN"
BASE COUNT          983 a         1808 c         1532 g          976 t
ORIGIN      
        1 gagtacctgg cgacggcgac ctgatctcag gcccgttcgc ccagaaaaca ggtctcttca
       61 aattccgaac gatcaagaga gggaacgcaa ggggctgtcc caaggctccc ggaatgcccc
      121 gaaaagtgcg aaacacaagt ctaagatcgg ttcgccgctc tcgcagaatg gcaaaccagt
      181 tctcgcaagt ggtgaactga ccggacacta acgctttgaa agatcgtaca cgggctatcg
      241 aaccttccgc tgttcaggtt gcgtatgccg ccctcattct gccgcgccac cagaaggaag
      301 caagaggaaa cttgaaattc ctcccctttc gcccccgttt ccacactatt cagcctgact
      361 ctctaggggg tcacatcttc cgtactcttc gcttctgctg ccgatcatcg accttcccaa
      421 ttcctaaagg ttgccagcgg cgtctcctca gcagctgtgg ggtcggtatt ccgacagctc
      481 ttcgcgtgga agcagactgc ccagacggga gctacatccg atgctccctg ttcatcatgt
      541 caagggccgg cagttcacgc aagccgcatg gtggtcgaat ctccgcggtt agccgtgcca
      601 gttcctcctc aaccgactcg gcatggacct ctgcctcggc ctgccggtcc cactccgcca
      661 actccccaag ctcgtccggc gtcggctggt actcttcggc cagcgtggcg aagaatcgca
      721 gggagccgac gaaggcgcgc ttgggcggca gcttcttctc cagcacggcg attcggctct
      781 tgaggctcat gcgatcctcg gcttcggagc cggttggggt tgggcggctt ccagcttcgc
      841 gacgcgctcg gccagttcta cggtttcacg cagcttgcaa ccgagttcga tgacggcacg
      901 cgccgccgac agccggacca tctcggactt gcccttcctg ctcaggtatc ccagcgagtc
      961 ggacgcactc gccatgttgt cgatcagccg gccgagggcc tgtgaggtca tttcggatcg
     1021 gagtccctcc acccgtcgcc ggaaccccgg cacgtcggcg agccagagct tcaccgtgcg
     1081 gacggcagcg ccgcattcgc gggcggctac ctcgatggtg cagccggatg ccagggacat
     1141 agccgcggcc tcctgcctcg gtgataaacg ccgctccccg gtgcagtttg gggcagatgg
     1201 gtgcatggtg tcctccctct gtgatggccg ctgaggtttc accccgcggc acgccgagga
     1261 tcgcctccgc agggccgcga gacgttctga aaaaccacca acatagcaaa agcgacctgt
     1321 gcgtcagtcg ctcctcttac aagcggcgtg attcctcagt tctctcgggt taccgagagg
     1381 gcgacagacc acgttgtttc aggtgatgaa cgaggagaat ggcagtagtg gcagtagtgg
     1441 cagtagtggc agtaatctcc tccgatggcg ggaccgcgat ttctgcaatc ccaagcaccc
     1501 cctcctccga ggcagaaaag gtccccttgg atggagcttc tctactgcca ttactgccat
     1561 tactgccacc cccttaaagt ggctcgctcc aagtttgggc caaactactg ccattactgc
     1621 cattactacc atcagacatg agcctactgc tgggtgacca cgcgccatcg caccccggcg
     1681 ctcacggcgt caacccgctc cagcatcatc ccaccgatca cgcgcccgcg aaggtggtgc
     1741 agtttcatcc ccagagatct tgcattgact atttgccctc gcccctcggt agggcagagg
     1801 acaaacagtg cttcgcgaaa agctagaata tctggatcgg attcgttatg acaataaagc
     1861 agcatatcat tcacggtcaa tcccgctccg ttcgtgtcca gatatgcaat ccccttgagc
     1921 aaccctgcaa gtgcgattga ctcggggctt gaatcgcggc gtagttcctt gcgagcgtcg
     1981 ccggggtcat ccaatccgca ccagaccacg gcgttgcgga ccaggctcgt ccacccctcg
     2041 aacgaacccc atggcgtgag ctgctgatcg gggcggtcgg cgacacaata agcgcgcaag
     2101 attgtcaaag ccgccgacag cagctcacct cggttgtttc ggacgtgctt gcgcaagtct
     2161 ggatacttga aatcgccccg atcctccggg cattcaaggg gcgattcgag acgaataggc
     2221 atgacacggc gcggcgtgtc gccgatgagg tacacgttgt tgccagttgc ataccatgtg
     2281 acgtttaaag gtccgttgaa ttgattgttg ccgccgagga tgcggtcctt ccaccagctc
     2341 gcagtcagtg cccgatccag cacggcgttg ccgacgaagc cgctcaggtt gtcgagcagg
     2401 accatttcgt caccctccat cgccagaggc gtgatcgcct tccgtagctc ctctttgtcg
     2461 ttggtgtagc tcataacgga agcttccctt ccagtgacaa tgaggaagcc cacatcggcg
     2521 agcaatccct ttcccacacc agccgcgttg ccatcgagga ggatcagtgg cgatggccct
     2581 tgaaaggcgt agcgtgccag tggcgtcagc acggaagcga gccaagccgc cttgtggcat
     2641 tcggccttga aagggaagtc cgttaccacg tcacccagta acgtcatggc tgacacagcg
     2701 tcgtctctgg tcggttggtc ggagacctcc accccaagat cgtccggcat ccacaggagt
     2761 agaccggatt tctcatcata gcccggctca cgcagcagac tcccatctgc gagcagcgcg
     2821 gggtgcgtga caacgccttc caggtgtgga acgttttccc atgccccccg gcagtggatg
     2881 gttgcgacaa catggcccgg cagcgatgct ggcctgtgct caacgccctc tttgaccttc
     2941 accagccgga cgcaatcaac cagcttcgtg agttcctcac ggagcaaagc ctggggcaaa
     3001 gcgcggacga tgggagcggt ggaggggcgg cggactagcg ccttctcgct ggacggatca
     3061 tgctggacca cgtgcgcgag atggccacca cgttggtaca acccttccgc gttctctagc
     3121 gccgcttccg cttcggcatt cacacggaac tcgtcggtgc ccaagacgac cagcgcccga
     3181 tcaaggactg gccgggcctt ctccaccaat tcgcggaagg catcgggtcc gaaggcagcc
     3241 aggaagtcgt cgtcacccat cttgcagcca tctggccccg gtggtttttc cactgcgagg
     3301 acttccttca caccggcttt ccgcaaggct ctggccagtc tacgcagcgc tccagcaacc
     3361 tttctttgcg tttcgggctt cggctcgtag tcaaacccga tatgacagac tcctccgagt
     3421 ctggcgaggg ccagcaactc cgggatcaac tcttcactgc ctttcacctt ccagcagaag
     3481 acacccccga tgcccacggc caacaggccg gtctgagcta cggccagcgt ttttttttct
     3541 ccctgctctt tccgcgacat ccttaacatt aggctgctag atcgagcagg cgatcggctg
     3601 ccgctgcaat ttcccgctcc gtcaccccgg ggcgtagaac cgctcggatt tcctcgccca
     3661 acaactcccg ccgccaggca cgccgcttgt ccgcatggct gggccgcctt ggctcggcat
     3721 cccacgggga cgcacagcgg tgtcccacca acccctcctc gtgccgatcc caggcccaca
     3781 cctcagtcat cgtgaacgtc cacaggcaga cgtggaacgc ccccacactg gcccatacga
     3841 accgcacctg ctgctgacct gcgccgacca cctccttgca atcccggaac gccgtctcca
     3901 gggagaaccg gtcggccaca cacccgagga tgtcggccac acccgccgac gggtcgctac
     3961 agaagtacgc cacccaaccg gccggctcgt ccacgacgac cacccggatc gcacccccgg
     4021 ccggacgcca cgtcgccacg aacgtcttgt agcgtttcgt cgtctccttt ccgtagagga
     4081 tgaacgtgct ggtcgcccat ccccgccgct gaccggcccg cttggccagc tcaacccggc
     4141 tctccccgta gacccggggc cggccgcgcc gcccggggcg atgccgcgga agtccccaca
     4201 gggcggagtc tttgcggagc cggctgacca ccgtcacccc caggccccgc atcggcttga
     4261 ggaacggggc cttggcataa gccccgtcgg ccaccaccca cagccgctgg cctaggaacc
     4321 cgagccaggt ggccgcccaa cgcatcagtt cgacggccag ctccagcttg gtccggaacg
     4381 gcggccggtg ccgcgcgtcg atgctgccca ggtttttccg ccggacgtag agccgcgcca
     4441 gcagcggcag ggcgatcgtg ccccacagcg ggtggccgac gagcaacccg aggacgaccc
     4501 agacatgccc gtacacgaag gggctgccgg ccgggccggg tgctgggttg tggtgaacgc
     4561 cggccccttg aacctgcggc ccgtaacgag gcgtcggcgt gtcatcgagg gcgaagatca
     4621 gacggccgga ggccgccgcc acccgcttca cgacctccca cgccagccgg gcggcgatgg
     4681 cgtcggcccg cttcccggcg gcggccaccg tcgtgtacac gggcttgtag tcgctgctca
     4741 ggccggccgc gcgaatccaa ctggtgaccg tccgtcggcc acgggccagc atcgccccca
     4801 cgaacaccaa cgccaggcga acagcggagc gtcggtcgag ggccgtagcg aggcgggaaa
     4861 accattggca tggggcaggg gtcgtttgcg agaatggcat ggccgagtcc gtccggggcg
     4921 gaggttggtg tggcaaccac catcgtcgcg gacgggccgg caatcgtcca ccgattcgtc
     4981 agaggagcct cggcgccatg cggccgatcg acgtgagaaa cctgatccgc ttcaatacgc
     5041 gcatgggcat gttcatctgc ccgtcgaacc ggcccaacgt cgtcgcgttc gtccacggct
     5101 accagtacgc cacggccacc gagtgtcggt tcaccgagct actcagcgcc cacatcgccg
     5161 accgataccg cgtcaaggcc gacgcactcg ggtggcccca tcaaatcgcg cgcttcgccg
     5221 aacggcgttc gcttgattgg atggatgcct atctcctgct cagttctgaa ctcctgaact
     5281 cggccatcgg cgcaaacga
//
LOCUS       DASZYN010000287        12192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_779114, whole genome shotgun sequence.
ACCESSION   DASZYN010000287 DASZYN010000000
VERSION     DASZYN010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 12192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12192
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779114"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1441)
                     /locus_tag="VHE33_07425"
     CDS             complement(<1..1441)
                     /locus_tag="VHE33_07425"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HVT97324.1"
                     /translation="MSSQPVTSVPPQQKQASGKSAPGKTGLVASLGLFSATAIVVGSM
                     IGSGIFLVPADISRATGSPALLIGAWLVTTVMTIIGALSYGELAAMMPTAGGQYIYLR
                     EALGKIWGFLFGWTLFLVIQTGTIAAVAVAFGKFLGVFFPSVSTTNWVWHIGHVPPWH
                     VGPMVLGNMDIGISTANLAGIVIILLLAVVNVFGVKLGALVQNVFTTAKTVALLGLVV
                     LGVLFMRNASAIHANFGANFWHGAGWHSLHAVQVGAGGPVAMVGLLTILAVVQTGSLF
                     SADAWNNVTYTAGEVKNPKRNLPLSLALGTSLVLGLYILANFVYLMALPLHGDPHGST
                     IAARGIQYASEDRVATAALQQIFSHAGAYLMAGAILISTFGCANGLTLAGARVYYAMS
                     RDGLFFKSAGKLHPKWHTPVAALIVQALWTCVLCISGSYSQLLDYIICTELIFYILTI
                     ACLYVLRAKRPNAERPYRAIGYPVLPAVYI"
     gene            complement(1455..2117)
                     /gene="thiE"
                     /locus_tag="VHE33_07430"
     CDS             complement(1455..2117)
                     /gene="thiE"
                     /locus_tag="VHE33_07430"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HVT97325.1"
                     /translation="MPDFGGRNIPRLYPILDAEMVLRDGSDNRGNRETLLRRIAAELA
                     DAGVQILQYRNKRDDDETVAEDARVIREAATGITLILNDRAALAATLGWDGVHIGQQD
                     MPASEARHRVGPSAWVGLSTHNEEQTRAAALEPVDYIAIGPVFATSSKANPDPVIDLE
                     GVRRARGLTRKPLVAIGGITADTAAAVIDAGADAVAVIGAVFASGHSVSHSVRDFLAK
                     FK"
     gene            complement(2195..3097)
                     /locus_tag="VHE33_07435"
     CDS             complement(2195..3097)
                     /locus_tag="VHE33_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97326.1"
                     /translation="MKSQLFGAAMLAFAVAAGAQTSAAGSESASAAAQAGHASASAAQ
                     ATNVSAELTKKIESKDAKPGDPVFAKTTSEAHLSDGTKIPKGSKLLGHVTDVQAKSKE
                     NHDGHVAFCFDHAVLKDGREVPVNAMVRLLSAPAEVAPSAGPDDLMASGGMQASSPMT
                     GGGGGGLAANGPGGAVHSAAGLAGGATSTAGSGLHTAGNGIDGAVNGTTTEGGALGAG
                     VRGAGGAALNGSAGVGAGANGAAMPVGNLSGVTFATVHLAADAGTGGTSATGGASTAT
                     LVTGHNRNVSLYSGSQMTMSIAPR"
     gene            complement(3358..3570)
                     /locus_tag="VHE33_07440"
     CDS             complement(3358..3570)
                     /locus_tag="VHE33_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97327.1"
                     /translation="MLPSVKTTHVIPRQLPPVRLATVSTFLRCPFCMRTIGAYKSPAQ
                     RIRLEAKHICSDKAVVGMPAVSMPFN"
     gene            3889..4890
                     /locus_tag="VHE33_07445"
     CDS             3889..4890
                     /locus_tag="VHE33_07445"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVT97328.1"
                     /translation="MSNAITVSTSPIVLVGSANERLAEILGSTLESWHYQVEPVQRGE
                     DALARLDGLNPPSIALLDMDLPEPSAAEVVWEVRRRQEQRRTWNILLSERRDSERVRA
                     ALQSGADDFLVFPAESTDSSDLRVRLRVAERVVALTSRIQKQSSELRYHATHDSLTGL
                     WSREALISLIFQETDRVQRMKTPLSLMLLDLDDFSRINHDYGYETGDRILMEVASRFR
                     RQLRSYDLIGRAGEDEFLLALPGCSLENALHLAGRIRETLLGRPFATDRKSAAITASI
                     GVAASGGRSPMVVLREAERALAEAKLSGKDCARGDAPEAWLTQPVLAAGVPPGMNGD"
     gene            5104..5721
                     /locus_tag="VHE33_07450"
     CDS             5104..5721
                     /locus_tag="VHE33_07450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVT97329.1"
                     /translation="MEEIAGSAASIFYGTTDAGSEVAIEKREQAMLELADFDEVVRVY
                     RPRILRFLLSSLTDRDAAEALTQECFLKAWSARHQFRGDSSLSTWLTRIAVNLMRDHL
                     RSRSLRFWQKTRGSSLDVMDISDWIPDGRSSPESVAVARDRVAGVWRAVNDLSAHQRS
                     VFVLRFVEEMELEEIATTIGMNLSTVKSHLYRALAAIRERLGETK"
     gene            5718..6179
                     /locus_tag="VHE33_07455"
     CDS             5718..6179
                     /locus_tag="VHE33_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97330.1"
                     /translation="MKFRKDAAANPELDSSRDDIRDLAEDLSLYRSAMHHLAESEAAR
                     RPFVAEARPAHSFHLRLLLAPALAAAVAAGVFVPVYSHFHHRHPAVATASQAAPDPTE
                     ARASIDDSALMNQIDSDLSENVPDALQPLADLSDQATTKNSVSENKNATHE"
     gene            6163..6807
                     /locus_tag="VHE33_07460"
     CDS             6163..6807
                     /locus_tag="VHE33_07460"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HVT97331.1"
                     /translation="MPRMNNYVLAVLLCLLAPGFAVAQSFDGPPPGPGGPDPMQGPGT
                     PGPPMERAFHAEHRGRWWDDPQMAQQMNITDQQKKQMDDIFLQHRLKLIDLHANLDKQ
                     ETLLQPMIEADQPDEAKILSQIDAVAQARAELEKANAHMLFDIRKTLTPDQWQKMKAM
                     REEHHKMMRGGPDGRGGPDGRMWRKHRPGPPPDGTAPAPPPAPQGNGAPPSANE"
     gene            7296..9191
                     /locus_tag="VHE33_07465"
     CDS             7296..9191
                     /locus_tag="VHE33_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HVT97332.1"
                     /translation="MAHVLHPETTESNPLNTEMETPTLEAPPEQSEPAAETTSNPETA
                     ETAPHVVAKAAPSELAAESSDHMEEPDLSMEDFAAALESFDREQAAEAAAQAFDDSAI
                     VTGTVIKLTDKHVVVDVGMKSEGLIPIEQVVDHTGQPKLKAGDSVDVVIEREEPEGGY
                     LLGYEKAQRLRVWDTIEKAHNEKTALTGTVVGRVKGGLTVDIGIKAFLPGSQLELRPV
                     RNLDAWIGQPIQVRVIKLNKKRGNVVVSRKEILEEEQTERRSMTLEHLEEGAVLTGTV
                     KNLTDYGAFVDLGGIDGLLHITDMSWGRLTHPRDLVNVGDEIQVKVLKFDKEKQRVSL
                     GFKQLTPDPWLDAAERYPIGAHVKGRVLSVTDYGAFVELEQGIEGLVHVSEMTWSKRM
                     KHPSKLVKPGDEVETVILAVNPADRRISLGMKQLLDNPWEHLADKYPTGATVEGRVRN
                     LTDFGAFIEIEDGIDGLVHVSNLSWTKRVKHPSEVLKKGEKVRAVVLGVEPENRRLSL
                     GIKQLQPDVWETFFAQHRVGDVVKGKVLRTAQFGAFVEIAEGVEGLCHVSEATDGHGA
                     PVKLEQGQEMEFKIIRMNPEEKKVALSIRAVSGDEASRADVEAYKQPVSSSTTTLGDL
                     VHWKREK"
     gene            complement(9273..9653)
                     /locus_tag="VHE33_07470"
     CDS             complement(9273..9653)
                     /locus_tag="VHE33_07470"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVT97333.1"
                     /translation="MNSDVRFSLPARRRAILAGCLMALTLLAALPATGRAQNFGLKNL
                     QGKVYDANNAPISGAIVYLKNSRNNDVKTFLSEKDGNYRFAGLSADTDYSVWAAYHAK
                     KSDSKTVSSFDSRKQVYIDLKIKE"
     gene            complement(9668..9856)
                     /locus_tag="VHE33_07475"
     CDS             complement(9668..9856)
                     /locus_tag="VHE33_07475"
                     /inference="COORDINATES: protein motif:HMM:NF044773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97334.1"
                     /translation="MPICPECESALDFDEEEVEEGDVVVCDECGTEYEIVATDPLELS
                     KMEEGYEDEEELGEEEEE"
     gene            10062..11594
                     /locus_tag="VHE33_07480"
     CDS             10062..11594
                     /locus_tag="VHE33_07480"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HVT97335.1"
                     /translation="MIVSRAAACIVLSTAVFAAQAQSRASSPDASHHDLDAIGAPIHP
                     MPADPAIAHAIAQVSPAQIQHTIATLVSFHNRSTLSSLDSDLPAHTGVLAAADWIESQ
                     FKAISAACNGCLQVRRDSFIQPPLTGPNPRVTQPTPLHNIYAILRGSDPTQASRMILV
                     TGHYDSRDSTNEDTHGEAPGANDDASGTAVSLECARVLSKLKLPGTLVFVAVAGEEQG
                     LLGSKHLADLAKKEGWQLEGVLNNDIVGGNHTPGDKFQDFHAVRVFSENVPANAPPEE
                     IRRILALGYESDSPSRELSRAIADVGRTYTAAPLVASLDPVLEFRRDRFLRGGDHSSF
                     NQDGFAAVRFTEWREDFDHQHQNVRVENGVQYGDLLKYDNFDYIARVARLNAATMAVL
                     ASAPPPPVSVQYPPPPRVFTIALNNTEITWDQAPETPAGTKYEIVWRPLAAPDWTRSI
                     LADKLASVADGKFSATDGKYTITIPVSKDNVVFGVRAVDAKGHRSPAVVPWPAPRPTR
                     AE"
     gene            complement(11700..>12192)
                     /locus_tag="VHE33_07485"
     CDS             complement(11700..>12192)
                     /locus_tag="VHE33_07485"
                     /inference="COORDINATES: protein motif:HMM:NF044544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97336.1"
                     /translation="VTLGDVETMVLAAKQRSLYELTDAISAKDRARALSLLHGLLNAS
                     DGGEDSAIGHLYMLARTFRQMLVILEKNVRDSRAIWQALWQGFRMPPFAAEDVIRQAR
                     RYKSRRELTRALRLIARADLELRSQPPDKRLVLERLVLDLASEPKAAVGVGLSPQYAF
                     SDF"
BASE COUNT         2294 a         3761 c         3916 g         2221 t
ORIGIN      
        1 atatatagac ggcaggcagc acgggatagc cgatggcgcg ataaggccgc tcggcgttgg
       61 ggcgcttcgc gcgcagcacg tagaggcacg cgatggtgag gatgtagaag atcagctccg
      121 tgcagatgat gtagtcgagc aactggctgt aggagccgga gatgcaaagc acgcaggtcc
      181 acagcgcctg cacgatgagc gcggccacgg gcgtgtgcca cttcggatgc agtttgcccg
      241 cggacttaaa gaacaggccg tcacggctca tggcgtagta gacgcgcgcg ccggccaggg
      301 tgaggccgtt ggcgcagccg aaggtggaga tgagaatagc gccggccatc agataagcgc
      361 ccgcgtggga gaagatctgc tgcagggcgg cggtcgcgac gcgatcttcc gacgcgtact
      421 ggatgccgcg ggcggcgatc gtggacccgt gcggatcgcc gtgcagcggc agcgccatca
      481 gatagacgaa gttcgccagg atatagaggc cgagtaccag agaagttccc agcgccagcg
      541 acaggggcag attccgcttt gggtttttta cctcgcccgc cgtgtaggtc acgttgttcc
      601 aggcgtcggc ggagaacagc gagccggtct ggacgacggc gagaatcgtg agcaggccca
      661 ccatggcgac cggtccgccc gcacccacct gcacggcgtg gagactgtgc cagccggcgc
      721 cgtgccagaa attcgcgccg aagttcgcgt ggatggcgga ggcgttgcgc atgaacagca
      781 cgccgagaac gaccaggccc agcagcgcca ccgtcttggc ggtggtgaag acgttctgga
      841 cgagggctcc taacttcaca ccgaaaacat tgacgacggc gagcagcagg atgatcacga
      901 tcccggccag attggcggtc gagatgccga tgtccatgtt gccgaggacc atgggaccga
      961 catgccaggg gggaacatgc ccgatgtgcc aaacccagtt tgtggtcgaa accgagggga
     1021 agaagacgcc gaggaatttt ccgaaggcga cagcgacggc ggcgatggtg ccggtctgga
     1081 tgacgaggaa gagcgtccag ccgaagagga agccccagat tttgcccagc gcctcgcgca
     1141 ggtagatgta ctggccgccg gcggtgggca tcatcgcggc cagctcgcca tagctgagtg
     1201 cgccaatgat ggtcatcacg gtcgtcacca gccatgcgcc gatgagcaac gccggcgagc
     1261 cggtggcgcg gctgatgtcg gccggaacca ggaagattcc ggaaccaatc atggagccga
     1321 caacgatagc agtggcgctg aagagaccca ggctggcgac gaggccggtt ttgccgggag
     1381 cggactttcc cgaggcttgt ttttgctgag gagggacgga cgtaacgggc tgggaactca
     1441 cgtgtcctac agttctactt gaattttgcg agaaagtctc tgaccgagtg gcttacggaa
     1501 tggccggatg cgaaaaccgc accgatcact gcgactgcat cggcacccgc gtcgatgacg
     1561 gccgctgcag tgtctgccgt aatgccgcca atcgcgacca gcggcttgcg ggtgaggcca
     1621 cgcgcacggc gtacgccttc gagatcgatg acgggatcgg ggttggcttt gctgctcgtc
     1681 gcgaacacgg ggccgatggc gatgtagtcc accggctcaa gggcggcagc tcgggtttgc
     1741 tcctcgttat gggtggagag gccgacccac gcggatggac cgacccgatg ccgcgcctcg
     1801 ctggcgggca tgtcctgctg gccgatgtgc acgccatccc agcccagggt tgcggccagc
     1861 gcggcgcggt cattgaggat cagcgtgatt ccggttgcgg cttcgcgaat gacgcgagca
     1921 tcttcggcca cggtttcgtc gtcgtcgcgc ttgttgcggt actgcaggat ctggacgccg
     1981 gcatcggcca gctctgcagc gatgcggcgc agcagagttt cgcgattgcc gcggttgtca
     2041 gacccatcac gcagaaccat ctccgcatcg aggatcggat agagccgcgg aatgtttctg
     2101 ccgccgaagt ccggcaattc catcctccag gagaaaagcc ctgccacgcc gtagcgtagc
     2161 agggcttgtc aggagtttcc cgcggtggag gaatctaccg gggcgcaatg ctcatcgtca
     2221 tctgcgatcc actgtagagg ctgacgttgc ggttgtgccc ggtcaccagg gtggccgtcg
     2281 aagcgccgcc ggtggcgctg gttccgccgg ttcccgcatc ggctgccagg tgcactgtgg
     2341 caaaggttac gccggaaaga tttcctaccg gcatcgccgc gccgttcgca ccggcgccta
     2401 cgccggccga gccgttcagg gctgctccac cggctccgcg aacgccggcg ccgagggctc
     2461 cgccctccgt ggttgttccg ttcacggcac cgtcgatgcc gttgccggcg gtatggagtc
     2521 cgctgcccgc ggtacttgtg gcgccaccgg ccagacctgc cgcgctatgc actgcgccgc
     2581 ccggtccatt ggcggccagg ccgccgcctc cgccgccggt catgggactg ctggcctgca
     2641 tgcctccgct ggccatcaga tcgtccgggc ccgcagaggg agcgacttcc gccggcgccg
     2701 acagcaagcg caccatcgcg ttcaccggca cttcgcggcc atccttcagc acggcgtggt
     2761 cgaagcagaa cgccacatgg ccatcgtgat tctctttcga tttcgcctgc acatcggtca
     2821 cgtgtcccag cagcttcgat cctttcggaa ttttcgtgcc atcggaaaga tgcgcttcgc
     2881 tggtggtttt ggcgaagacc ggatcgccgg gcttcgcgtc tttgctctcg attttcttcg
     2941 tcagctccgc ggagacattg gtggcctgtg ccgcggaggc tgaggcgtgt cctgcctgcg
     3001 ctgccgcgct ggccgattcg cttcctgcgg ctgacgtctg cgcaccggct gctactgcaa
     3061 aagccagcat ggccgcccca aagagttgtg atttcatgtt gaacctcgtt ccgagacgtt
     3121 cctgtgatcc cacggtcgcg atggtttggg atcttctggc gcggtacgat gccagagccc
     3181 tttccgcggt tggggagatg cgaaggatgc tagctgcaac tttggaggaa tgacaacagg
     3241 aacgggcccg gaagggccct cgatcaaaaa gaaagcggtt gcagaacgaa gacagcgcct
     3301 ggtgtatcaa cggcgcacac ctttacggtc gcggcggccg gggaggaatc ggccacccta
     3361 gttaaagggc atcgagacgg caggcattcc aaccacagcc ttgtcgctgc agatatgttt
     3421 cgcttccagg cggatccgct gcgcgggaga cttataagct ccgatggttc tcatgcagaa
     3481 cgggcagcgc aggaaggtcg aaaccgtcgc cagacgcacc ggagggagtt gcctggggat
     3541 gacatgcgtg gtcttgactg atgggagcat cgctttctcc taaacagagt cgcgacgtgc
     3601 gggccctgaa gttttcgtgg tggtggtgat ggctctttcg tctctgatta tggcacgcaa
     3661 acgtcacatc gaaagcgtga agttgtgtaa attttgatgt gaccaatgat tactattggc
     3721 tctgtttggt cccgatgcgg cacattcccc taactgggga tttgtgcggt atccgaactt
     3781 agtgaggggg aaaagatgcc ctgatttacg gcagattcgc gcctttgtgc atttcccacg
     3841 tccaggtgcc gatagttgcg tcaggcaatt ttgtctggag ccgggatatt gtcgaatgca
     3901 attactgtca gcaccagtcc catcgtcctt gtagggtcgg ccaatgaacg gctggcagaa
     3961 attctgggga gcaccctgga gtcctggcac tatcaggtag aaccggtgca gcggggggaa
     4021 gacgctctgg cgcgcctgga cgggctgaat ccgccgtcga tcgcgctgct ggatatggat
     4081 ttgcccgagc cgagcgcggc ggaagttgtg tgggaggtcc gccggcgcca ggaacagcgc
     4141 cgcacctgga acatcctgct gagcgaacgt cgcgacagtg agcgagtgcg ggcggcgctc
     4201 cagtccggcg ccgacgactt tcttgtgttt ccggcggagt cgaccgattc ctcggatctt
     4261 cgggtccgac tgcgggttgc ggaacgggtt gtggcgctga ccagccggat tcagaagcag
     4321 tcttcggagc ttcgctacca tgccacgcac gacagcctga cagggctgtg gagccgggaa
     4381 gcgctgatca gcctcatttt tcaggaaacc gaccgtgtgc agcggatgaa gacgccgctg
     4441 agcctgatgc tgctggatct ggacgatttt tcgcgcatta atcacgacta cgggtacgag
     4501 acaggggatc ggatcctgat ggaggttgcg agccggttcc gccgacagct gcgcagctac
     4561 gacctgatcg ggcgggccgg ggaggacgag tttttgctgg ccctgcctgg ctgctcgctg
     4621 gagaatgcgc tccacctggc cggacggatt cgggagacgc tgctgggtcg tccgtttgcg
     4681 acggacagga aatctgcggc gatcacggcg agcatcggcg tggccgccag cggggggcgt
     4741 tccccgatgg tggtgctgcg ggaggccgag cgcgcgcttg cggaggccaa gctttccggg
     4801 aaagactgcg cgcgcgggga tgcgcccgag gcctggctga cccagccggt gctggcggcg
     4861 ggagtcccgc ccgggatgaa cggcgactga ggtttgcggg gcacagagcg cagggcgcag
     4921 ggcacagggc acagggtgca gggcgcactg ggcagcgcac agcgggcagg gcacagggcg
     4981 ccgttgcggg tagcccggcg agcgtcgccc tccgaccttt tcagaaccgc ctgcgccctg
     5041 cgatccgcga atctggcccc tggcctgtaa actcgccgcg tagaatcccg tctatctcgt
     5101 cgcatggaag agattgcagg cagtgcggcg agcatcttct acggcacaac ggacgccggc
     5161 agcgaggtcg ccatcgagaa acgggagcag gccatgctgg agctcgccga cttcgacgaa
     5221 gtcgtgcgcg tttaccggcc gcgcattctg cgctttctcc tctcgtctct caccgaccgc
     5281 gatgctgcgg aagcgctgac ccaggagtgt tttctcaagg cctggagcgc gcggcatcag
     5341 tttcgcggcg attcgagcct gagcacctgg ctgacgcgca tcgctgtcaa cctgatgcgc
     5401 gatcatctgc gcagccgcag tctgcgcttc tggcagaaga cgcgcggctc gtcgctggat
     5461 gtgatggaca tcagcgactg gatcccggac gggcgcagct cgccggaaag tgtggcagtt
     5521 gcgcgcgatc gggtggccgg agtctggcgc gcggtgaatg acctgtcggc gcatcagcga
     5581 agcgtgttcg tgctgcgctt tgtcgaggag atggagctgg aggagatagc aaccaccatc
     5641 gggatgaacc tgagcacggt gaagtcccat ctgtatcggg ctctcgcagc cattcgcgaa
     5701 cgcctgggag aaacgaaatg aaattccgta aagacgctgc tgccaacccg gaactggact
     5761 cgagccgcga cgacattcgc gacctggcgg aggatctgag cctctaccgc tccgccatgc
     5821 accatctcgc cgaaagcgag gccgcccgcc gccctttcgt ggctgaggcg cgtcccgcgc
     5881 attcgttcca tctgcgcctg cttctggctc cggctctggc ggccgctgtg gccgcggggg
     5941 tgttcgtacc ggtctacagc cactttcacc accggcaccc ggcagttgcg acagcgagcc
     6001 aggcggcgcc cgatcccacc gaagcacgcg ccagcatcga cgattccgcg ctgatgaacc
     6061 agatcgacag cgacctttct gaaaatgtgc ctgatgctct gcagcctctg gccgacctga
     6121 gcgatcaggc caccactaag aattctgtat cggagaataa aaatgccacg catgaataac
     6181 tatgtgctcg ctgtgctgct ttgcctgctc gctcctggat tcgcggttgc gcaaagtttc
     6241 gacggacctc cgcccggacc gggcggcccg gaccccatgc aggggcctgg cacgcccgga
     6301 ccgccgatgg agcgcgcctt ccacgcggag catcgcggcc gctggtggga tgacccacag
     6361 atggcccagc agatgaacat caccgatcag cagaagaagc agatggacga catctttctg
     6421 cagcaccggc tgaagctgat cgatctgcat gccaacctgg acaagcagga aacgctgctg
     6481 cagccgatga tcgaagccga tcagccggat gaggcgaaga tcctgtcgca gatcgatgcg
     6541 gtcgcgcagg cgcgagccga actggagaag gccaacgcgc acatgctctt cgatatccgc
     6601 aaaacgctca cgccggatca gtggcagaag atgaaagcca tgcgggaaga gcaccacaaa
     6661 atgatgcgcg gcggcccgga tggacgcggc ggaccggacg gccggatgtg gcgcaaacat
     6721 cgccccggac ccccgccgga tggcacggca cccgcgcctc caccggctcc gcagggcaac
     6781 ggagcacctc cctcggcgaa cgaataggcg gttgcaatcc ccggcaactc aagaggagcg
     6841 gttttcaccg ctcctctttt ttccacaaaa gtcaacagcg ctggtgtcta cttccgtaac
     6901 taaagttccg ggcaatttgc tcttgatttc acgcgacttc aattgacact ctgatagcgc
     6961 agcactatag tttttacagg tggtttgcgc gctcgggaca ctgcgccgac ctccggtcgc
     7021 cactcctgtt ccttccagtc ctgccggctg cttcagtcag gagagtccga tgtgcgatct
     7081 tgaccgcgca ccgttctccg atgggtaact ccgcgtggac tcggactcga tcagccggga
     7141 cgatccccga acttcgtcga gaaccgcgcc agggacccac gccctcccgc tgacggcgaa
     7201 gaaagctgca gctcggtgat ccggcaaggg tacatgctgg attcgtggct gcggggcgga
     7261 atccgggaac cgccgcatgc gtcccggagc tgtctatggc acacgtcctt catcctgaaa
     7321 cgaccgagag caatcctctg aacaccgaaa tggaaacgcc aacgctggaa gccccgccgg
     7381 aacagtccga accggcggct gaaaccacct ctaaccccga gaccgccgaa accgcgccgc
     7441 acgtcgtggc caaagctgca ccgtctgagc tggctgccga atcttccgat cacatggaag
     7501 agccggacct gagcatggaa gacttcgcgg ctgcactgga gtcgtttgac cgcgagcagg
     7561 ctgcggaagc cgccgctcag gccttcgatg acagcgcgat tgtcaccggc acggtgatca
     7621 agctgaccga caagcacgtc gttgtcgacg tgggcatgaa gtcggaaggt ctcatcccga
     7681 tcgaacaggt cgtcgaccac accggccagc ccaagctgaa ggccggcgac agtgtcgacg
     7741 tggtcatcga acgcgaggag ccggaaggcg gctacctgct tggctacgaa aaggcccagc
     7801 ggttacgcgt gtgggacacg atcgagaagg cgcacaacga aaagacggcg ctgacgggga
     7861 cagtggtggg acgcgtgaag ggtggcctca cggtcgacat cggcatcaag gcgttcctgc
     7921 ccggttcgca gctggagctg cgccctgtgc gcaatctcga cgcgtggatc ggccagccga
     7981 tccaggtccg ggtcatcaag ctgaacaaga agcgcggcaa cgtggtcgtc tcccgcaagg
     8041 agattctcga ggaggagcag actgagcggc gctccatgac cctggagcat ctggaggaag
     8101 gcgcggtgct caccggcacg gtgaagaacc tcaccgacta cggcgctttc gtggatctgg
     8161 gcggtatcga cgggctgctg cacatcaccg acatgtcgtg ggggcggctc acgcatccac
     8221 gcgatctggt caacgtcggc gatgagattc aggtcaaggt tctgaagttc gacaaagaga
     8281 agcagcgcgt ttccctcgga ttcaagcagc tcacgcccga tccatggctc gacgccgcgg
     8341 agcgctatcc catcggggca cacgtgaagg gccgggtgct gagcgtgacc gactacggcg
     8401 ctttcgtcga actggagcag ggcatcgagg gcctggtgca cgtttctgaa atgacgtggt
     8461 ccaagcgcat gaagcacccc tcgaagctgg tgaagccggg cgatgaggtg gagacggtga
     8521 ttctggccgt gaatccggca gaccggcgga tttcgctggg catgaagcag ctcctcgata
     8581 atccgtggga gcatctggcc gataagtacc cgaccggagc gaccgtggaa gggcgggtgc
     8641 gcaacctcac cgacttcggc gcgttcatcg agatcgagga cggcatcgac ggcctggttc
     8701 acgtgagcaa tctgagctgg accaagcgcg tgaagcatcc gtcggaggtg ctgaagaagg
     8761 gcgagaaagt tcgcgctgtg gtgctgggtg tagagccgga gaaccgccgt ctctcgctgg
     8821 gcatcaagca gttgcagccc gacgtttggg agacgttctt cgcgcagcat cgcgtgggcg
     8881 atgtggtgaa gggcaaggtg ctcaggaccg cgcagtttgg cgccttcgtt gaaatcgcgg
     8941 aaggagtcga gggcctgtgc cacgtgtccg aagctacgga cggccacggt gcgccggtga
     9001 agctggaaca ggggcaggag atggagttca aaatcatccg gatgaacccg gaggaaaaga
     9061 aggtcgctct cagcatccgg gcggtcagcg gcgacgaagc cagccgcgcc gacgtggaag
     9121 cctacaagca gcctgtctcg agctccacga ccaccctggg cgatctggtc cactggaaac
     9181 gcgagaagta agctccggaa cggtaccgaa tgaaaacgcc acccgaaagg gtggcgtttt
     9241 tctgtgtccg gaggcctggg ccccgggggc tgtcattcct tgatcttcag gtcgatgtac
     9301 acctgcttgc gcgagtcgaa cgagctcacg gtcttcgagt cgctcttctt cgcgtgatag
     9361 gccgcccaga cgctgtaatc ggtgtcggcc gaaaggccgg cgaagcggta gtttccgtcc
     9421 ttctccgaga gaaacgtctt cacgtcgttg ttgcgcgaat tcttcagata cacgatcgcg
     9481 ccgctgatgg gcgcattgtt ggcgtcatag accttcccct gcaggttctt aaggccaaag
     9541 ttctgcgcgc gtcctgtggc cggcagcgcg gccagcaacg tcagagccat caggcagccg
     9601 gccagtatgg cccggcgccg cgcaggcaga gaaaacctta cgtcagaatt cactcgcact
     9661 ccttgcctca ttcctcttcc tcttcgccca gttcttcctc gtcctcgtag ccctcttcca
     9721 ttttgctcag ctccagcgga tcggtcgcca cgatttcgta ctccgttccg cattcgtcgc
     9781 aaacgaccac atcgccctct tcaacctctt cttcgtcgaa gtcgagcgcg ctctcacatt
     9841 cagggcatat tggcatgaat ccctccacct caaaaaaacg atcgtggctc catctgaatg
     9901 acgccactca cagggaaaat gttgttgcca ttttccgtat tcgtccaccg tatccaaacc
     9961 ttccggtcgg ttccgcggcg ccttcgctag ttttgaagag aattgccctg ggcgtcaagg
    10021 gttgtgcgcc actcttcttc catgaggcat actcgatagt catgatcgtt tcccgtgccg
    10081 ccgcctgcat tgtgctgtct actgccgttt tcgccgccca ggcgcagtcg cgcgcttctt
    10141 ctcccgatgc ctcgcaccac gatctggatg ccatcggcgc gccgatccat cccatgccag
    10201 ccgatccggc gattgcgcac gccatcgccc aggtctcgcc cgctcagatt cagcatacga
    10261 ttgccacgct cgtctcgttc cacaatcgca gcactctgtc cagtctggac agcgacctgc
    10321 cggcccatac cggggtcctg gctgcggccg actggatcga gtcacagttc aaggccatct
    10381 cggcggcgtg caacggctgc cttcaggtcc ggcgcgattc cttcatccag ccgccgctga
    10441 ccgggcctaa tcctcgcgtc acgcagccaa cgcccttgca caacatctac gccatcctgc
    10501 gcggcagcga ccccacacag gcctcgcgca tgatcctggt cacgggtcac tacgattcgc
    10561 gcgacagcac caatgaggac acgcacggcg aggcgccggg ggccaacgac gacgcgagcg
    10621 gtaccgcagt gagtctggag tgcgctcgcg tgctctccaa gctcaaactg ccgggtacgc
    10681 ttgtttttgt tgctgtggcc ggcgaagaac agggcctgct gggaagcaaa cacctggcgg
    10741 acctcgcaaa gaaagagggc tggcagctgg aaggggttct caacaacgac atcgtcggcg
    10801 gcaatcacac cccgggagac aagtttcagg atttccatgc tgtgcgcgtc ttctcggaaa
    10861 atgttccggc caacgcgccg ccggaggaga ttcgccgcat cctggcgctg ggctatgaga
    10921 gcgattcgcc ttcgcgcgaa ctttcgcgcg cgattgccga tgtcggtcgt acgtacaccg
    10981 ccgcacccct ggtcgcgagt ctcgatcctg tcctcgaatt ccgccgcgac cgcttcctgc
    11041 gcggcggcga ccactcctcg ttcaatcagg acggcttcgc cgctgtccgg tttaccgaat
    11101 ggcgcgaaga cttcgatcac cagcaccaga acgtgcgcgt tgagaacggc gtgcagtacg
    11161 gcgacctgct gaaatacgac aacttcgact acatcgcccg cgtagcgcgc ctcaatgcgg
    11221 cgacgatggc cgtgctcgcc agcgcgccgc ccccgcccgt cagcgtccag tatcccccgc
    11281 ccccgcgcgt cttcaccatc gccctgaaca atacggagat cacctgggat caggcgccgg
    11341 agacgccggc gggcacgaag tatgaaattg tctggcgtcc gctcgccgcg ccggactgga
    11401 cccgctcgat cctcgccgac aagctcgcct cggtcgcgga tggaaaattc tcagcgacgg
    11461 atggcaagta caccatcacc attccggttt cgaaggacaa cgtcgtcttc ggggttcggg
    11521 cggtcgatgc aaaaggacat cgcagcccgg cggtggtgcc gtggcctgct ccgcgtccga
    11581 cacgggcgga gtagcctctt tctgtttgct ggaaatccga gtggcccatc tgatcgcctg
    11641 gtcctggagc aggtgcgggc atcaggcgcg gcgcgattgg gaagaatcca gggttaggac
    11701 tagaagtcgc tgaaggcgta ctgcgggctc aatcccactc caactgcggc cttcggctcg
    11761 ctcgcgagat cgagcaccag ccgctccagc accaggcgct tatccggcgg ctgcgaacgc
    11821 agctccagat cggcgcgcgc aatcaggcgc agcgcgcgcg tcagctcgcg ccgtgacttg
    11881 taacgccggg cctggcggat cacatcttcg gccgcaaacg gcggcatgcg gaaaccctgc
    11941 cacagcgcct gccagatcgc tcgcgagtcg cgcacgttct tttccagaat caccagcatc
    12001 tgccggaacg ttcgcgccag catatagaga tggccgatcg ctgagtcctc tccgccgtcg
    12061 gaagcattca gcaatccgtg cagcaggctc agggctcgcg cacgatcctt cgctgagatc
    12121 gcatcggtga gctcgtagag cgagcgctgt ttcgcagcga gcaccatcgt ctccacgtcg
    12181 ccgagcgtga cc
//
LOCUS       DASZYN010000288         4650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1233059, whole genome shotgun sequence.
ACCESSION   DASZYN010000288 DASZYN010000000
VERSION     DASZYN010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233059"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1733)
                     /locus_tag="VHE33_07490"
     CDS             complement(<1..1733)
                     /locus_tag="VHE33_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HVT97337.1"
                     /translation="MKWPGEETRAWLRPRLTLSGAFVRERSWPFIFDLSVAAALLAGF
                     YAILWIAKFWFSSAIPEVEIYRSPSHLPLYAFYSLVRIFIAYGLSLLFAVGYGYTAAY
                     NKRLEGLMIATLDILQSIPVLSFLPGVMLAMMSLFPSRQLGIEFGAILLIFTGEVWNM
                     AFSFYTSLKSIPRELLEATEINRFSRWQRLFQLELPFGAIGLVWNSMVSVASGWFFLM
                     VCEMFPVGSRNFRLPGLGSYLQTAAEHGDWIAMAWGLITMVLIIVITDQLLWRPLIAW
                     SDKFKFENVESGSRVTSPVLDALRSSSVVGLLRRTTFKPMAERFYMEQAARRQRRAKP
                     AATGRKRNTLAIYAVADVVVLAAIGYAAFHAMLLLKAIHLHELLALLGGAGLTFLRVN
                     TALVIGSLWTIPVGVAIGFNPRLSQIAQPLAQIAASFPATALFPLILVALTQIGIGLG
                     AGSVVLMLLAAQWYILFNVIAGAQAIPSELREVARLFRFEDWQNWTTVILPGIFPFLI
                     TGLITASGGAWNASIVAEYFEVKGHTMATLGLGAQISQASSDANYAVLLAATMIMALM
                     VVTINRLVWRPL"
     gene            complement(1730..3100)
                     /locus_tag="VHE33_07495"
     CDS             complement(1730..3100)
                     /locus_tag="VHE33_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HVT97338.1"
                     /translation="MTDPIIQARQVEKYYAQSRDARIQVIAATDLAIYPGEIVALLGP
                     SGSGKSTLLRVLSGLSRPSAGHVLWHGKDLATTEVNVSIVFQSFALFPWLTVLENVEA
                     PLKARGMEAHQRHRRSLKILDTVGLDGFEAAYPKELSGGMRQRVGFARALVVEPEVLF
                     MDEPFSALDVLTAENLRSELLELWQKKTIPTQSIFLVTHNIEEAVLLADRIIVLGRNP
                     GHVRTDFKVQLAQPRDRKTQAFTALVDYIYKVLTRPDVTPTEVPVLADGRRVRDQRQM
                     RYEMLPHARPGGIAGLLEIIIDFNGRTDIYRLADELAFEIDDLFPIVEAAQLLGFLKV
                     EEGDAAITPEGREYAESEILKQKELFRQAALNHVLLLRQISRALANKSDHSVSEEFFL
                     DMMDEQFSEDECSRQLETAINWGRYAELFDWDDARRRFTMHVHEGDEPAETVSEPETE
                     HREKAG"
     gene            3353..3724
                     /locus_tag="VHE33_07500"
     CDS             3353..3724
                     /locus_tag="VHE33_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT97339.1"
                     /translation="MSEQQAKPKVLVADDERVIADTLAIILNQAGFEATAVYSGEKAV
                     EMAEILKPDMLISDVIMTDLNGIDAAIQIRAMLPGCKILLFSGQAATADLLDRVRTQG
                     HEFEILAKPVHPQDLLARLRS"
     gene            complement(3802..>4650)
                     /locus_tag="VHE33_07505"
     CDS             complement(3802..>4650)
                     /locus_tag="VHE33_07505"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HVT97340.1"
                     /translation="LTGPFRYDFYIGSLKGHTYPNDPWVHVEKVSFKPYRDLEFGFSR
                     MVVWGGKGHEPITLHTFLKSFFSFQNVTEAEKFSRSDPGARFGNFDFEWRLPHLQKWI
                     TLYSDSFVHDDVSPISAPRRAGVHPGIYLAQFPYLHQLDLRLEGANTEPASHASPNGS
                     PIDQGQFLYVETVQKQGATNKGFLPDWVGREGKGGQAWLTWHLSGNENIQFMYRNAKA
                     SARFIPGGTTQNDFAGSVVKRIGPGIEIKGVVQHESWKAPVYKTGSQSDTTAAAQITW
                     YPKSRE"
BASE COUNT          947 a         1658 c         1195 g          850 t
ORIGIN      
        1 agcggccgcc acaccagccg attaatcgtc accaccatca gcgccatgat catcgtcgcc
       61 gccagcagca cggcatagtt cgcgtcactg ctcgcctggc tgatctgcgc gcccaggccc
      121 agcgtcgcca tcgtgtgccc cttcacctcg aagtactccg ccacaatcga cgcgttccat
      181 gcaccgcccg atgccgtaat cagcccggtg atcagaaatg gaaaaatgcc cggaagaatc
      241 accgtcgtcc agttctgcca gtcttcaaac cggaacagcc gcgccacctc gcgcagctcc
      301 gacggaatcg cctgcgcccc cgcaatcacg ttgaacagga tgtaccactg cgccgccagc
      361 agcatcagca ccaccgaccc tgcgcccagc ccgatcccga tctgcgtcag cgccaccagg
      421 atcagcggga acagcgccgt cgccgggaac gatgccgcaa tctgcgccag cggctgcgca
      481 atctgcgaga gccgcggatt gaaaccgatg gccacgccca ccggaatcgt ccacagcgaa
      541 ccgatcacga gcgctgtatt gacccgcagg aaggtcagcc ccgctccccc aagcaatgcc
      601 agcaactcat ggagatgaat cgccttcagc agcagcatcg catggaacgc cgcgtatccg
      661 atcgccgcca gcaccaccac atccgccacc gcatagatcg cgagggtgtt tctctttcgc
      721 cccgtggccg ccggcttcgc tctccgctgc cgccgcgccg cctgctccat gtagaagcgc
      781 tccgccatcg gcttgaacgt ggttcgacgg aggagcccaa ccacgctcga gctccgcagc
      841 gcgtcgagca ccggcgaggt cacccggctg ccgctctcca cgttctcgaa tttgaatttg
      901 tccgaccacg cgatcagcgg ccgccacagc agctggtccg tgatcacaat gatcagcacc
      961 atcgtgatca gtccccatgc catggctatc cagtcgccat gctccgccgc cgtctgcaaa
     1021 tacgatccca gccccggcag cctgaagttt cggctcccca ccggaaacat ctcgcacacc
     1081 atcagaaaga accagccgct ggccaccgat accatcgagt tccacaccaa accgatcgcg
     1141 ccaaacggca gctccagttg gaacagccgc tgccagcgcg agaagcggtt gatctccgtc
     1201 gcctccagca gctcgcgcgg aatgctcttg agcgacgtgt aaaaactgaa cgccatgttc
     1261 cacacttcgc cggtaaagat cagcaggatc gcgccgaact caatgcccag ctgccgcgac
     1321 gggaacagcg acatcatcgc cagcatcacc cccggcagga agctcaaaac cgggattgac
     1381 tgcagaatgt ccagcgtcgc gatcatcagc ccttccaggc gcttgttgta cgccgccgtg
     1441 taaccgtagc ccacagcaaa gagcaaactc agcccgtagg cgatgaaaat ccgtaccagc
     1501 gaatagaacg cgtacagcgg cagatgcgac ggcgaccggt aaatctccac ctcgggaatc
     1561 gccgagctga accagaactt cgcgatccac agaatggcgt agaaccccgc cagcagcgcc
     1621 gccgctacgc tcaggtcgaa aatgaatggc cagcttcgct ctcggacgaa ggcacccgag
     1681 agcgtcagac gcggacgcag ccacgcgcga gtttcctcgc cgggccactt catcctgcct
     1741 tctcccggtg ctcggtttcc ggctcgctca cggtctctgc cggttcgtca ccctcatgca
     1801 cgtgcatcgt gaaccgccgc cgcgcgtcgt cccagtcgaa cagctccgca taccggcccc
     1861 agttgatcgc cgtctccagc tgccgcgagc actcgtcctc gctgaactgc tcatccatca
     1921 tgtcgaggaa aaattcctcc gataccgagt ggtcgctctt gttggccagg gcgcggctga
     1981 tctgccgcaa cagcaacacg tgattcagcg ccgcctgccg gaacagctcc ttctgcttca
     2041 gaatttccga ctcggcgtac tcccgcccct ccggcgtgat cgccgcgtcg ccttcctcca
     2101 ccttcaggaa gcccagcagc tgggccgctt ccacgatcgg gaacagatcg tcaatctcga
     2161 aagccagttc gtcggccagg cggtaaatat ccgtcctgcc gttgaagtcg atgatgatct
     2221 ccagcaagcc ggcaatcccg cccggccgcg catgcggcag catctcgtag cgcatctgcc
     2281 gctggtcccg cacccgccgc ccatcggcca gtaccggcac ctcggtcggt gtcacatccg
     2341 gccgcgtcag caccttgtag atgtaatcca ccagcgccgt aaaggcctgc gtcttgcggt
     2401 cgcgcggctg cgccagttgc accttgaaat ccgttcgcac atgccccgga ttccgcccca
     2461 gcacgataat ccgatcggcc agcaaaaccg cttcttcaat gttgtgcgtc accagaaaga
     2521 tcgactgcgt cggaatcgtc ttcttctgcc atagctccag cagttcgctg cgcaggttct
     2581 ccgccgtcag cacatccagc gccgaaaacg gctcgtccat gaacagcact tccggctcca
     2641 ccaccagggc tcgcgcaaac cccacacgct gccgcatccc gcccgacagc tccttcggat
     2701 atgccgcctc aaaaccgtcc agaccgacgg tgtccagaat cttcagactc cgccggtgac
     2761 gctggtgcgc ctccatcccc cgcgccttca gcggcgcttc cacgttctcc agcaccgtca
     2821 gccacggaaa cagcgcgaag ctctggaaaa cgatcgagac gttaacctcg gtcgttgcca
     2881 ggtccttccc gtgccaaagc acatgcccgg ccgaaggccg cgacagcccc gacagcacgc
     2941 gcagcaaagt cgatttaccc gaccccgatg gccccagcag ggcgacgatc tcgcccggat
     3001 aaatcgccag atcggtcgcc gcaatcacct gaatgcgcgc gtcccgcgac tgcgcgtagt
     3061 acttctccac ctgccgtgcc tggatgatgg gatcggtcat gaaagtcagg ccagttactc
     3121 ccgcgaattc cgggattttc gcttagcata gtgggtcgaa cgaaaggttt ccgttaagat
     3181 ttctcagtct gaaacttgtt gagcgggaac tgcccaggcg aaggcatctc ccgagaaaag
     3241 cgtatcatca tagctgcagg ttatcccgcc tcgactgccc caggagtctg cgcggcgatt
     3301 ctgcagttcc catattcatt cttttggacc tcaaggaagc ggtaagagac gaatgtccga
     3361 acagcaggcg aaacccaaag tcctcgtcgc ggacgacgag cgcgtgattg ccgataccct
     3421 tgccatcatt cttaaccagg ctggattcga ggccaccgcc gtctacagcg gcgagaaggc
     3481 cgtcgagatg gctgagattc tcaagcccga catgctcatc agcgacgtca tcatgaccga
     3541 cctgaacggc atcgacgccg ccattcagat tcgtgccatg ctccccggct gtaagattct
     3601 tctcttctcc ggccaggcgg ctaccgccga tctgctcgac cgcgtgcgta cccagggcca
     3661 cgagttcgag atcctcgcca aacccgttca tccccaggat ctgctcgccc gcctgcgcag
     3721 ctgaattctc cgcgaaaaag agagcaccgt gccgacaccc ggtgcctcct tcgcggctac
     3781 ctattctcta tcccctgttc cctattccct gcttttcgga taccacgtga tctgcgctgc
     3841 ggccgtcgtg tcgctttgcg aaccggtctt atagacagga gccttccagc tctcgtgctg
     3901 gaccacgcct tttatctcga tgccaggccc aatccgcttc accaccgagc cggcaaagtc
     3961 attctgcgtc gtcccccccg ggataaatcg cgccgaggcc ttcgcgttcc gatacatgaa
     4021 ctgaatattt tcattgcctg acagatgcca ggtcagccac gcctgcccgc ccttgccctc
     4081 gcgccccacc caatccggca gaaatccctt gttcgtcgca ccctgcttct gcaccgtctc
     4141 gacgtacaga aactgtccct gatcgatggg cgagccattg ggactcgcat ggctcgcagg
     4201 ttcggtgttc gcaccctcca gccgcaaatc cagctggtgc agatagggaa actgcgccag
     4261 gtaaatcccc gggtgcactc ccgctcgccg cggcgccgag atggggctca catcgtcgtg
     4321 cacgaaagaa tccgaataca gcgtgatcca tttctgcaga tgcggcagcc tccattcaaa
     4381 atcgaagttg ccaaaacgcg cgcccggatc gctgcgtgag aacttctccg cctccgtcac
     4441 gttctgaaaa ctgaaaaagc tcttcaggaa cgtgtgcagc gtgatcggct cgtgtccctt
     4501 gcctccccac accaccatcc tgctgaagcc gaactccaga tcgcgatacg gcttgaagct
     4561 gaccttctca acatgcaccc acggatcgtt gggataggtg tgccccttca ggcttccgat
     4621 gtagaagtca tagcgaaagg gtcccgtcag
//
LOCUS       DASZYN010000289         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_330865, whole genome shotgun sequence.
ACCESSION   DASZYN010000289 DASZYN010000000
VERSION     DASZYN010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330865"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VHE33_07510"
     CDS             complement(<1..417)
                     /locus_tag="VHE33_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT97341.1"
                     /translation="MQITLANKTAVVTGASSDIGLAVTRRYLECGATGVVAVFRRAQI
                     PEELIACQKEFGDRLHIVRGDVAEESTAIEFTQAALERWGRLDVLVSNAAKSVVKAIH
                     EHTPEEWDSVMNANVKAMYWASRHVIPVMIRQNGGLI"
     gene            complement(679..1407)
                     /locus_tag="VHE33_07515"
     CDS             complement(679..1407)
                     /locus_tag="VHE33_07515"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HVT97342.1"
                     /translation="MDDRFGRKNPIALNLQTAPQAAAQAIRESIISGELAGGDRIVEQ
                     KWAARLGIGQPTLREALHELEHQGLLRKLPQRGTYVAQLSPEDYRLIQEVRIPLEAIA
                     VGKAAENFTDEADEELTRLVTTMASSNSNKMNVKQFHDCDVSFHRKIWDLAGNDFLRH
                     TLETVTFRLFIFSVVGRWTNNPRALDESLASVQQHLGILQGLRTRDKNLARQAFIRHT
                     VQYWNAQYQLGLSEAELSEVYMSV"
     gene            1604..>2115
                     /locus_tag="VHE33_07520"
     CDS             1604..>2115
                     /locus_tag="VHE33_07520"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HVT97343.1"
                     /translation="MNFSALNRRDFLRTLAATGIASAAGTISAETQAGSATGSANLAS
                     NTAESMALHRLNPLTLAQVVVDDEFWSPKRRVWQQVTIRDCFRKFENYQTGTLNNFDR
                     VKAGERGHHAGDPWWDGLIYEMIRGSADFMLSHPDPELEAQIDGYIERIAAAQAIRPD
                     GYINTYTQLA"
BASE COUNT          430 a          637 c          597 g          451 t
ORIGIN      
        1 aatcaggccg ccattctggc ggatcattac cggaatcaca tgccggctcg cccagtacat
       61 ggcttttaca ttggcgttca tcaccgagtc ccactcctcg ggagtgtgct catgaatcgc
      121 tttcaccacg cttttggcgg cgttgctcac cagcacatcc aggcgtcccc agcgctccag
      181 tgctgcctga gtgaactcga tggccgtgga ttcctccgcg acatccccgc ggacgatgtg
      241 cagccgatct ccaaattcct tctggcaggc aatcaactcc tcaggaatct gcgctcgccg
      301 aaacacggcg acgacgccgg tcgccccgca ttcgagataa cggcgggtta ccgccagtcc
      361 gatgtcggaa gaagccccgg tcacaacagc ggtcttgttg gcgagagtga tctgcatgag
      421 acggacctcg tgatcgcaag agcgaaggga accttgcgtt gtcgctatag atcataaata
      481 aacgcttgaa cgtgtcaacg ggtttcgggc tgacgcccca agctgtcacc tgcctcacgc
      541 ctacaacgcg aactgagttt gacctgggaa tggcccgggt tatgacggct ctggcatcgc
      601 gcaaatgaga cgcggcatgt gcggcaggtt gttgaacgcg tgagcagggg atgcgaagtg
      661 tcgatggaag ggcgttgact aaacgctcat gtagacctcg gacaactctg cttcactcaa
      721 tccgagctgg tactgagcgt tccagtactg caccgtgtga cggatgaaag cctggcgcgc
      781 gagattcttg tcacgcgtgc gtaatccctg cagaattccc aggtgctgct gcaccgaagc
      841 caggctctca tcgagcgccc tgggattgtt ggtccagcgg ccgaccacgg agaagatgaa
      901 caggcgaaat gtgaccgtct ccagcgtgtg ccgcagaaaa tcgttgcccg cgagatccca
      961 gatcttccgg tggaaagaga catcgcagtc gtggaactgc ttcacattca tcttgttgct
     1021 gttactgctg gccatcgtcg tcaccagccg cgtcagctct tcatcggctt cgtcggtgaa
     1081 gttctcggcc gcctttccca ccgcgatcgc ttccagcgga atgcgtactt cctggatcag
     1141 ccggtagtcc tccggactga gctgggcaac gtaggtcccg cgctgcggaa gtttgcgcag
     1201 cagcccctgg tgctccagct cgtgaagggc ttcccggagt gttggctgtc cgatgccaag
     1261 ccgcgcggcc cacttctgtt ccacgatccg gtcgccgccg gccagttctc cggagatgat
     1321 cgattcccgg attgcctgtg ccgccgcttg cggggcggtc tgcaaattga gagctatcgg
     1381 attctttcga ccaaaccggt catccatgca gaagatcctg agttcggcgc atgatgatct
     1441 ttattaaagg atagagccgg gtttcaagca agctcgcgca cgcttctcct gatccacccc
     1501 cgcagcggca gctgtaaatg agacctctcg cggacacgaa cgagtagtac ctaccttgac
     1561 gatttgtcaa tctataatat ataacgcctt ctggagttcg actatgaatt tcagtgccct
     1621 gaatcgtcgt gattttctgc gcacgctcgc cgccaccgga attgcctcgg ccgccggtac
     1681 gatttcggct gagacgcagg ccggatcggc gacgggatcc gcgaacctcg cttcaaacac
     1741 cgccgaatca atggctctgc accggctcaa tccccttacc cttgcccagg ttgttgtgga
     1801 tgatgagttc tggtcgccga aacggcgggt ttggcagcag gtcaccatcc gcgattgctt
     1861 tcgcaagttc gaaaactacc agaccggaac cctgaataat tttgatcgcg tcaaggcagg
     1921 agaacgcggc catcacgccg gcgatccgtg gtgggacggt ttgatttacg agatgatccg
     1981 gggctcggcg gacttcatgc tcagtcatcc cgacccggaa ctggaagcgc agatcgacgg
     2041 atatatcgag aggatcgcag cagcccaggc catccggcct gacggctaca tcaacaccta
     2101 cacccaactc gcgga
//
LOCUS       DASZYN010000290         3587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_269090, whole genome shotgun sequence.
ACCESSION   DASZYN010000290 DASZYN010000000
VERSION     DASZYN010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269090"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..550
                     /locus_tag="VHE33_07525"
     CDS             251..550
                     /locus_tag="VHE33_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97344.1"
                     /translation="MDDYVVEVLMRDLVGHDRRPVSFLVYLWLSAAQARAGREVQISF
                     QEMAESVGVSKSAAQAAVRWLIRRKLLAASKSSATAVPRYKVLTPWKDSARRVRG"
     gene            592..939
                     /locus_tag="VHE33_07530"
     CDS             592..939
                     /locus_tag="VHE33_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97345.1"
                     /translation="MVCGAALAFGALVTGCQSPWIACTVVNHQSAPVFLVEVNYPGGS
                     FGVQTIAPGGTFRYRFHSLSTDMVSVDFTDAAHKSHTVKGPRLLQRQEGTLQIEIEPD
                     NKVSWTPVLKNAP"
     gene            complement(953..1282)
                     /locus_tag="VHE33_07535"
     CDS             complement(953..1282)
                     /locus_tag="VHE33_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT97346.1"
                     /translation="MKVARIILFTAQMEAMSRFYGEVLGLKRVTDEKGWREFAAGSLR
                     IALHSGPASPGRKGPKIAFYSTDVTAVRDKLVARGARFGKVRDAGTFVLCDGKDPDGN
                     WIQLSSR"
     gene            1401..2189
                     /locus_tag="VHE33_07540"
     CDS             1401..2189
                     /locus_tag="VHE33_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVT97347.1"
                     /translation="MIRVENLTKTFGDFTAVKDITFEVESGEIFAFLGPNGAGKTTTI
                     KMLTTLLRPTAGRIHLDGFDPWVKQREARQRFGIVFQDPSLDGEQTAYENMELHGILY
                     HVPHKVRVERIEQLLKLFELWERRKDLVKTFSGGMKRRLEIARGFLHTPKILFLDEPT
                     LGLDPQSRNQLWTHVKGLNEKEKTTVFLTTHYMDEADRVAHRIAVMDHGAIVAIGTSA
                     ELKAKTSTDSLENAFLALTGTSIRDEAANSTDQMRRFAQMWQRR"
     gene            2224..3009
                     /locus_tag="VHE33_07545"
     CDS             2224..3009
                     /locus_tag="VHE33_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT97348.1"
                     /translation="MGAIYIMWLRELKRYFRSKAQIVVSLGQPTLYLLALGFGLGPVF
                     AKAGAGNYIQFIAPGVIGMTVLFSSVFSGMAMLWDRQFGFLKETLVAPVPRIQIIFGR
                     TLGGATVASLQGLLMLIVCLIAGFRPVHASGGGYNWLALPLAGIFIGMIAIVFAALGA
                     SFGSTIKDMQGFQMLMNFLVMPLFFLSGALFPLTSLGKILSWITRIDPLTYGIDGLRG
                     VLIGNWQFGFNWQLNFMLDFCLLAALAAIFLSTSAWFFSRIEV"
     gene            3082..>3587
                     /locus_tag="VHE33_07550"
     CDS             3082..>3587
                     /locus_tag="VHE33_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HVT97349.1"
                     /translation="MISLLSVLLLFQDTNPVAPPVNNGSAIVEMIQQSGPIAMTVLIV
                     LLLGSFFSWAIILGKWASFRRARAQSQRFLRAFRKANRLQEIATVSEQFKPSPLVNVF
                     DEVYETYRRQTGGFGPPRNITALERAGQTAASESLTAMEQRLTWLASIGAIAPFVGLF
                     GTIMGIVDA"
BASE COUNT          684 a         1058 c         1075 g          770 t
ORIGIN      
        1 tccgcgcctg gaaccttcag ttcgatccag cgctgcttct cgttgaacgg ctggtccgtc
       61 gttaccttca gtcctaccgc ctcggtccag aacttcaatg cgcgatcctg gtcggtgacc
      121 ggaacgccca cgaatttgat gcctttgatc atgtttttct cctctcgatt tccggagcat
      181 aacgacagcg agaaaggggc gcaatcgtcc agggattgct atagtaacga gtgatataga
      241 gaggcccccg gtggacgact acgtcgttga agtgctgatg cgggacctgg tcggacacga
      301 ccgcaggccg gtcagcttcc tggtttatct gtggctgtct gccgctcagg cacgagcggg
      361 cagagaagtt cagatcagct tccaggagat ggccgagtcc gtcggcgtct cgaagagcgc
      421 agcccaggcg gccgttcgct ggctgatccg gcgcaaactg ctcgcggcga gcaagagcag
      481 cgccactgca gttccccgtt acaaagtact gacgccgtgg aaagacagcg cgcggcgggt
      541 gcgtggatga tagtctggcg gcgaatgaaa cgagagcgtg gcaggtgggc aatggtgtgc
      601 ggagctgcgc tggcgtttgg cgcgctggtc accggctgcc agtcgccatg gattgcctgc
      661 acggttgtta accatcaaag tgcaccggtt tttcttgtcg aggtgaacta tcctggcggc
      721 tcgttcggag tccagaccat tgcacccggc ggaacgtttc gttaccggtt ccactccctg
      781 agcaccgata tggtgagcgt cgatttcacc gatgcagccc ataagagtca cacggtgaaa
      841 ggtccccgtt tgctacagcg gcaggaaggc accctgcaga tcgaaattga gcctgataac
      901 aaggtcagct ggacgccggt cctcaagaat gcgccgtgat tccggctcac gctcagcggc
      961 tcgatagctg aatccagttg ccgtccgggt ccttgccatc gcagagaacg aacgttccgg
     1021 catcgcgaac tttgccgaac ctggcaccgc gcgccacaag cttgtcgcgc acggccgtga
     1081 catcggtcga ataaaacgcg atctttgggc cttttcgtcc cggcgatgcc ggacccgaat
     1141 gcagcgcgat gcgcaaggat cctgccgcga actcccgcca acccttttcg tctgtgactc
     1201 gcttcaggcc gagcacttcc ccgtagaaac ggctcatcgc ttccatctgt gcggtgaaca
     1261 ggataattcg tgccactttc atgcggcgct tctccgttaa ccaattgctg tggatttttt
     1321 aagcgtacag gatcgatgtt tctggcacac tagacggatt gtctcaactg caacttttct
     1381 tcatcgggag ggcacaggcc ttgattcgtg tagaaaacct gaccaaaacc tttggcgatt
     1441 tcaccgcagt caaagacatc accttcgagg tggagtccgg ggagattttc gcgttcctcg
     1501 gaccgaacgg cgcgggcaaa acgaccacca tcaagatgct gacgactttg ctgcgtccga
     1561 ccgccggccg cattcacctc gacggcttcg atccctgggt gaagcagcgt gaagcccggc
     1621 agcggttcgg catcgtgttt caggatccca gcctcgatgg ggagcagacc gcttacgaaa
     1681 acatggaact gcacggcatc ctctaccacg tgccgcacaa ggtgcgtgtg gagcggatcg
     1741 agcagttgct gaagctgttc gagttgtggg aacggcgcaa ggatctggtg aagaccttct
     1801 ccggaggcat gaagcggcgg ctggagatcg cgcgcggttt cctgcacacg ccgaagattc
     1861 tctttcttga cgaaccaacg ctggggctgg atccgcagag ccgcaaccag ctttggaccc
     1921 acgtgaaggg gctgaacgag aaggagaaaa cgacggtctt cctcaccacc cattacatgg
     1981 atgaggcgga ccgcgtggcg catcgcatcg cggtgatgga tcacggcgct atcgtggcga
     2041 ttggaacctc ggcggagctg aaggcgaaga ccagcaccga ctcgctggaa aatgcgtttc
     2101 tggcgctgac cggaacatcg atccgcgacg aagccgcgaa ctcaacggac cagatgcggc
     2161 ggtttgcgca gatgtggcaa cggcgataac ggcgcggtcg agggaggccg cgaaggagag
     2221 atcatgggcg cgatttacat catgtggctg cgcgagctga agcgatattt tcgctccaaa
     2281 gcgcaaattg tcgtatcgct gggccagccg acgctttatc tgctggccct gggatttggc
     2341 ctggggccgg tcttcgcgaa agcgggcgcc ggcaactaca tccagttcat cgcaccggga
     2401 gtgatcggca tgacggtgct cttcagttcc gttttttccg gtatggcgat gctctgggac
     2461 cgccagttcg gattcctgaa ggaaacgctg gtggcgccgg taccccgcat ccagatcatc
     2521 ttcggccgca cactgggggg cgcgacggta gccagcctgc agggtctgct gatgctcatc
     2581 gtgtgcctga tcgcgggctt caggcctgtt cacgccagtg gcggcggcta caactggctc
     2641 gccctgcctc tggctggaat cttcatcggc atgatcgcca tcgtctttgc tgctctgggc
     2701 gcttcgttcg gatcgaccat caaggacatg cagggattcc agatgctgat gaacttcctg
     2761 gtcatgccgc tgttcttcct gtcgggcgct ctgtttcctc tcaccagcct ggggaagatt
     2821 ctcagctgga tcacccgcat tgacccgctg acctatggca tcgatggcct ccggggcgtc
     2881 ctgatcggca actggcagtt cggcttcaac tggcaactca atttcatgct cgatttttgc
     2941 cttctggccg ctctggccgc catttttcta agcaccagcg cctggttttt ctcgcggatc
     3001 gaggtgtaac gaagcaggtt ctggctaggg ccgtgcaacc ccacaccaga ttcctgcttc
     3061 tgctaacgtg atagtcagac gatgataagt ctcctttccg tactgcttct ttttcaggac
     3121 accaatcccg tcgcgcctcc ggtcaacaac ggcagcgcga tcgtggagat gatccagcaa
     3181 agcggcccga tcgccatgac ggtgctgatt gtgctgctgc tgggcagctt cttctcctgg
     3241 gcgatcatcc tgggcaagtg ggcgtcgttt cgtcgcgcac gtgcgcagag ccagcgtttt
     3301 ctccgcgcct tccgcaaggc caatcgcctg caggaaatcg cgaccgtgag cgaacagttc
     3361 aagcccagcc cgctggtcaa tgttttcgac gaggtttacg agacgtaccg gcggcagacg
     3421 ggtggatttg gtcctccgcg caacatcacc gctctcgagc gcgccgggca aacggccgcc
     3481 agtgaatccc ttaccgccat ggagcagcgg ctcacctggc ttgcgagcat cggcgccatt
     3541 gcaccgtttg tcggactctt cggcaccatc atgggtatcg tggacgc
//
LOCUS       DASZYN010000291         5259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_76013, whole genome shotgun sequence.
ACCESSION   DASZYN010000291 DASZYN010000000
VERSION     DASZYN010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76013"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VHE33_07555"
     CDS             <1..619
                     /locus_tag="VHE33_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HVT97350.1"
                     /translation="YDAGFREARSAVDAEPVLQGAKEFASVAEAVADCTLVVGTAEGS
                     GRKTEEPLERLEAVGAAMLEALRDGARVAVLFGSEKTGLSNQNLSYCQRVLRIPTEAQ
                     QPSMNLGQAVAVVLYELIREGETFSAREETRLASAGDRERLVELLVEALERSRSGGQP
                     SGPTTEERVRRLLRTMALTEPDAQGLMGIVRQILWKLRGEETTSG"
     gene            complement(606..1211)
                     /locus_tag="VHE33_07560"
     CDS             complement(606..1211)
                     /locus_tag="VHE33_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97351.1"
                     /translation="MVPKTRRRVAAASTAAVALACFPSTARADAGIAMLPVTHGTMLF
                     FLLLVIIIEFIYLQARLKTRFRRTIVAIVTVNTATTGLGFPIAWGLYAILNSWAAFPA
                     GMAGVFTSAQFVPVWVTQKLNPDWSGMHGEGYVILGVFIVLLVPSYLFTRFLKTWVFE
                     WYDLLRYEGDIKPAVLAANRISYFLLGLTGCMILFRDFNRM"
     gene            1249..3414
                     /locus_tag="VHE33_07565"
     CDS             1249..3414
                     /locus_tag="VHE33_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVT97352.1"
                     /translation="MHLGVSNRVVTGCVAGVLAAVTGLGAAGQDRRGAADRDWPAYNG
                     GVDGDHYSPLTQINRANVGQLKVAWQFDTGEKGNLQTNPLVVGGVLYACTFSGKVVAL
                     DAATGHLLWSFDSSVENRDETIGGASQPSRGVAYWTDGKSERIFAGVMNYLYAVDAKT
                     GKAIADFGEGGRVDLRKGLRADLDAKYEQQSIALTTPGVVYKDLIIVGGRNPEAHPAP
                     PGDIRAYDVRTGALRWSFHTIPHPGEFGYDTWPADAWKDAGAANNWSGMTLDRERGIV
                     YVPTGSAAFDFYGGDRIGNDLFANTLLALDANTGKRLWHFQGVHHDIWDRDFPAPPAL
                     VTVTRGGKRVDAVAQTTKQGVVYVFDRMTGEPLFPIEEKGYPASEVPGEKASATQPMP
                     TEPAPFARQKLTADMLTTRTPEAHAWAAKEFATFRSEGPFIPMGVDRQTVVFPGFDGG
                     AEWGGPGVDPKTGVLYVNANEMANTGGLTVQDPNAKAGAKTYQAQCTMCHGANRAGSP
                     PAFPSLVGVSERLKDEQIAAVITHGRGRMPGQPNLDNAKVTELVAYLKTAVEEPKPAE
                     HVPGEKMGGGPAMQWAFTGYKKFLDPDGYPAIAPPWGTLSAIDLNTGKYLWKIPLGEY
                     PALAAQGMKDTGSENYGGPVVTAGGLVFIGATSYDRKFRAFDSATGKLLWETTLPFSA
                     GATPATYSVDGKQFVVIAASGGRDPRGPVGGVYVAFSLP"
     gene            3497..3985
                     /locus_tag="VHE33_07570"
     CDS             3497..3985
                     /locus_tag="VHE33_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97353.1"
                     /translation="MLLMPAGTILNYGAGMQFLRFSLRSRGLVAAAAITLFASSCALR
                     AQEPASTKNQTASSDSHLVSPSQLDSQVQTSTATRHQQVEGLTRFLSTPQAEKAMKDA
                     KIDPAQVKTAIPTLSDAELAQLSARADKAQHDFAAGGLSTLALALIIIAVVLIILLAA
                     YH"
     gene            4092..4643
                     /locus_tag="VHE33_07575"
     CDS             4092..4643
                     /locus_tag="VHE33_07575"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HVT97354.1"
                     /translation="MSRIVVLLCVIAAASFSQAQVQSAPGIWIDVPFVHQPREGCGAA
                     SLSMVMQYWARQQGHAAGADSDVAAIQRELYARSEHGIRASSMEKYLRAHGFEVFALS
                     GRWNDLETQLRKGRPLIVALRPAGQRELHYVVVDGVDAERGLVMMNDPAERKLLSQER
                     AGFEKDWKATEDWMLLAVPASAR"
     gene            4652..>5259
                     /locus_tag="VHE33_07580"
     CDS             4652..>5259
                     /locus_tag="VHE33_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT97355.1"
                     /translation="MVLACAGVGAQCSPAPGPDEARVEQLYQQKNWAEVVRVAGALHA
                     RSADVQFDYGMALAHLGRLAESRTELVAGAQACPGQERFPVELGGVAFEQKKYAEAAH
                     WLRRGLRIDPADAYANNFLGTVYFLSGNLEAALRYWNRVGKPEIAGLDFDPHLKVHRL
                     LLDRSFAFSPAAVLREPQLITTESRLEGLGIFPGYNLHLDARP"
BASE COUNT          982 a         1233 c         2102 g          942 t
ORIGIN      
        1 gtacgacgcg ggatttcgcg aggcgcgctc ggcggtggac gcggagccgg tgttgcaggg
       61 ggcgaaagaa tttgcgagcg tcgccgaagc cgtcgccgat tgcacgctgg tggtgggaac
      121 cgcggagggc agcggacgca aaacggaaga gccgctggag cggctggagg cggttggagc
      181 ggcgatgctg gaggcactgc gtgatggagc gagggtggcg gtgctgttcg gttcggagaa
      241 gacggggtta tcgaatcaga atctgagtta ttgccagcgg gtgttgcgga ttccgactga
      301 ggcacagcaa ccgtcgatga acctggggca ggctgtggcc gtggtgttgt atgagctgat
      361 ccgggaaggc gagacgttct cagcgcgaga agaaacgagg ctggcgagtg caggagatcg
      421 ggagcggctg gtggaattgc tggtggaggc gctggagcgc agcaggagcg gcggccagcc
      481 atcgggaccg actacggaag aaagggtgcg ccggttgctg cggaccatgg cgctgacgga
      541 gccggatgcg caggggctga tggggatcgt acggcaaatc ttgtggaagc taaggggcga
      601 ggagactaca tccggttgaa gtcgcggaac aggatcatgc agccggtgag gccgagcagg
      661 aaataggaga tgcgattcgc ggcgaggacg gcgggcttga tgtcgccttc gtagcggagg
      721 agatcgtacc actcgaagac ccaggtcttg aggaagcggg tgaagaggta ggaggggacg
      781 aggaggacga tgaagacgcc gagaatgacg tagccttcgc cgtgcatgcc ggaccagtcg
      841 ggattcagtt tctgggtaac ccagacggga acgaactgcg cgctggtgaa aacgcctgcc
      901 atgccggcgg gaaacgccgc ccaggaattg agaatcgcgt aaagtcccca ggcgatgggg
      961 aagccgagac cggtggtggc ggtgttgacg gtaacgatgg cgacgatggt gcggcgaaag
     1021 cgggtcttga ggcgggcctg caggtagatg aactcgatga tgatgacgag gaggaggaag
     1081 aagagcatgg tgccatgcgt gacggggagc atggcgatgc cggcgtcggc acgggcagtg
     1141 gacggaaaac aggcgagggc aactgcagcc gttgaagctg cggcaacgcg gcgcctggtt
     1201 ttcggtacca ttgccgggat gatagcaacg ggtttcaggg ggaggaaatt gcacctgggt
     1261 gtcagtaatc gtgttgttac ggggtgcgtg gccggtgtgc tggctgcggt gacagggctc
     1321 ggggcggcgg ggcaggaccg gcggggagcg gccgatcggg actggccggc gtacaacggc
     1381 ggggtggatg gggatcatta ttcgccgctg acccagatca accgggcgaa tgtggggcag
     1441 ctgaaggtcg cctggcagtt cgataccggg gagaagggga atctgcagac gaatccgctg
     1501 gtggtggggg gcgtgctgta tgcgtgcacg ttctcgggaa aagtggtggc gctggatgcg
     1561 gcgacggggc atttgctgtg gagctttgac tccagcgtgg agaaccggga cgagacgatc
     1621 gggggcgcat cgcagccgtc gcgaggagtg gcgtactgga cagatgggaa gagcgagcgg
     1681 attttcgcgg gggtgatgaa ttatctgtac gcggtggacg cgaagacggg gaaggcgatc
     1741 gcggactttg gggagggcgg gcgcgtggat ctgcggaaag gactgcgtgc ggatctggac
     1801 gcgaagtatg agcagcagtc gattgcgctg acgacgccgg gcgtggtcta caaggatctg
     1861 atcattgtgg gtgggcgcaa tccggaggcg catcccgcgc cgccggggga tattcgcgcg
     1921 tatgacgtgc ggaccggcgc gctgcggtgg agctttcaca cgattccgca tccaggggag
     1981 tttgggtacg acacgtggcc ggcggatgcg tggaaggatg cgggcgcggc aaacaactgg
     2041 tcggggatga cgctggatcg cgagcgcggg attgtttacg tgccgacggg gtcggcggcg
     2101 ttcgattttt atggaggcga ccggatcggg aacgatctgt ttgcgaacac gctgctggcg
     2161 ctcgatgcga atacgggcaa gcggctgtgg cattttcagg gcgtgcatca cgatatctgg
     2221 gatcgggatt ttcctgcgcc gccggcactg gtgacggtga cgcgcggggg caagcgcgtg
     2281 gatgcggtgg cgcagacgac gaagcaggga gtggtgtacg tcttcgatcg gatgacgggg
     2341 gagccgctgt tcccgatcga ggagaagggg tatccggcga gcgaggtacc gggagagaag
     2401 gcgtcggcga cacagccgat gccaacggag ccagcgccgt tcgcgcggca gaagctgacc
     2461 gccgacatgc tgacgacgcg cacgccggag gcgcatgcgt gggcggcgaa ggaatttgcg
     2521 acgttccgga gtgaggggcc gttcattccg atgggcgtgg acaggcagac agtggtcttt
     2581 cctgggttcg atggcggggc ggagtggggc gggcctgggg tggatccaaa gacgggcgtg
     2641 ctgtatgtga acgcgaacga aatggcgaat acgggcgggc tgacggtgca ggatccgaac
     2701 gcgaaagccg gtgcgaagac gtatcaggcg cagtgcacga tgtgtcacgg ggcgaatcga
     2761 gcgggatcgc caccggcatt tccgtcgctg gtgggggtga gcgagcggct gaaggatgag
     2821 cagatcgcag cggtgatcac gcatgggcga gggcgcatgc cggggcagcc gaatctggac
     2881 aacgccaagg tgacggaact ggtggcgtat ttgaagacgg ccgtcgagga gccgaaaccg
     2941 gcggagcatg tgccgggaga gaagatgggc ggcggaccgg cgatgcaatg ggcgttcacg
     3001 gggtacaaga agtttctcga tccggatggc tatccggcga ttgcgccgcc gtggggaacg
     3061 ctgagcgcga tcgatctgaa tacggggaaa tatttgtgga agattccgct gggtgagtat
     3121 ccggcgctgg cagcacaggg aatgaaggac acgggctcgg aaaattatgg tgggccggtg
     3181 gtgacggcag ggggcctggt gtttattggg gcgacgtcgt acgatcgcaa atttcgtgcg
     3241 ttcgacagcg ccacgggaaa gctgttgtgg gagacgacgc tgccgttttc ggccggcgcg
     3301 acgccggcaa cctactcggt ggacggaaag cagtttgtgg tgatcgcggc gagcggcggg
     3361 cgggatccga ggggaccggt gggcggagtg tatgtggcgt tctcgctgcc atgatgcggc
     3421 cagggctgtc gaatgcagga agcgggagtt gcgccaactg ggttctgttc gcgtggcatc
     3481 ctagtgaaga gtgtccatgc tgttgatgcc cgcggggacc atcctgaatt acggagccgg
     3541 tatgcaattt ttaaggtttt ctttaaggtc gcgggggctt gttgccgccg cagcgatcac
     3601 gcttttcgcc tcgtcgtgcg ctctgcgcgc acaggaacca gcctcaacga agaatcagac
     3661 ggcgagttca gactcgcatc tggtgagtcc ttcgcaactc gacagtcagg ttcagacatc
     3721 gacggcgacc cggcatcagc aggtggaagg gctgacgcgg tttctgtcca cgccgcaggc
     3781 cgagaaggcg atgaaggacg caaagatcga tccggcgcag gtgaagacgg cgattccgac
     3841 gctgtcggat gcagagctgg cgcaactttc ggcgcgtgcc gataaggcgc agcacgactt
     3901 tgcagccggc ggactgtcga cgctggcact ggcgctgatc atcatcgctg tagttctgat
     3961 cattcttctg gcggcgtatc actgaagaca gatcgcaggg ctcaggtcac agagcgcagg
     4021 aagagctgcc gcgcttcgcg cgatacccac atccccgacg agagggatgt ggggtaccga
     4081 ggattgaggc tgtgagtcgc atcgtcgttt tgctttgcgt gattgcggct gcgtccttct
     4141 cgcaggcgca ggtgcaatcc gcaccgggga tttggatcga tgtgccgttt gtgcatcagc
     4201 cccgtgaggg gtgcggggcc gcgtcgctgt cgatggtgat gcagtattgg gcgcggcagc
     4261 aggggcacgc ggcgggagcg gacagcgatg ttgcggcgat ccagcgcgaa ctctatgcgc
     4321 ggtcggagca tgggattcgt gcgtcgtcga tggagaagta tctgcgggcg catgggtttg
     4381 aggtgtttgc gctgagcggg cgttggaacg atctggagac gcagctgagg aaaggccggc
     4441 cgctgattgt ggcgctgcgg cccgcgggac agagggagct gcattatgtt gtggtggacg
     4501 gggtggatgc ggaacgcggg ctggtgatga tgaacgatcc ggcggagcgg aagctgttgt
     4561 ctcaggagcg tgcgggattc gagaaagact ggaaagcgac ggaagactgg atgctgcttg
     4621 ctgtgcccgc ttctgcacgc tgattgtcgt tgtggtgctg gcctgtgcgg gcgtgggcgc
     4681 gcagtgttca ccggcgccgg ggccggatga ggctcgggtc gaacaactct atcaacagaa
     4741 gaactgggcg gaggtagtgc gtgtggcggg agcgttgcac gcgcggagcg ccgatgttca
     4801 gttcgactac ggcatggcgc tggcgcatct tgggcggctg gcggagtcgc gtacggagct
     4861 ggttgcaggg gcgcaggcgt gtccgggaca agagcggttt ccggttgagc tgggtggggt
     4921 ggcgttcgag cagaagaagt atgccgaagc agcgcactgg ctacggcgcg gattgcggat
     4981 cgatccggcg gacgcttatg cgaacaactt tttggggacg gtgtattttc tgtcggggaa
     5041 tctggaggcc gcgctccggt actggaatcg ggtggggaag ccggagattg cggggctgga
     5101 tttcgatccg catctgaagg ttcacaggtt gctgttggat cggtcgtttg cgttttcgcc
     5161 ggcggcggtg ctgcgggagc cgcaactgat cacgacggag agccggctgg aggggctggg
     5221 gatttttcct ggatacaacc tgcatctgga cgcgcggcc
//
LOCUS       DASZYN010000292         5916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_653351, whole genome shotgun sequence.
ACCESSION   DASZYN010000292 DASZYN010000000
VERSION     DASZYN010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653351"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VHE33_07585"
     CDS             <1..490
                     /locus_tag="VHE33_07585"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT97356.1"
                     /translation="LIYLLREGYEVYAADADPQAVDKVRSMARQLAPPLPASNFRVEA
                     VEHLSFDDGCADVVISSAVLHFARDDAHFESMLLGSWQVLKPGGLFFCRLASSIGIED
                     QVERIEGRRYKLPDGSTRYLADEGMLRSWTERLGELADPLKTTVVQNQRSMTTWVIRK
                     SP"
     gene            860..1405
                     /locus_tag="VHE33_07590"
     CDS             860..1405
                     /locus_tag="VHE33_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HVT97357.1"
                     /translation="MLNAPGVVLIDPKYPHNVGAAIRACSCFGVDALVWTGSRIQLSQ
                     YERLPREERMKGYTRVHFLNHQRPFELFGDCTPVCVEVYENSEPLTLFEHPENAVYIF
                     GPEDGSVPQVIRRLCHRFVHIPAHFCLNLSAAVNVLLVHRLMTRQLAGKEPLLPLREM
                     LHEVRGDVPTPAMDAVGWDGK"
     gene            1450..2691
                     /locus_tag="VHE33_07595"
     CDS             1450..2691
                     /locus_tag="VHE33_07595"
                     /inference="COORDINATES: protein motif:HMM:NF020322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVT97358.1"
                     /translation="MPAFLKHILDGQNQFASGGLLLMILGSIGVFLRSLPQRLWWWLE
                     AQMTMMVTVKDDDDAFDWVKEWFLEQAFLRRVRRVDLDTTLRGAELALIPAPGRHWFW
                     RAGRPFWVWFYRSTESKGTSQRRMESLTFQTIGRDQKFLKAFVDEVVACHRRKRRSAS
                     YLYLYDDGWDYVAAYSPRSLDSVLLKPGEKEHLMEDLKRFRASRSRYARLGVPYHRGY
                     LLYGPPGTGKTSLVSAIGARFGMSIYVVNLTEMNDRTLKGAMNSVPENSIILFEDIDC
                     MKASNRRPELSPPAASLSGTTMRDGNNAPDRGGVTLSGLLNVLDGFHAPSNVAFVMTT
                     NAVEALDPALLRPGRIDYKLYLGEASDSQKYELYRRFFPEATDFEAREFVEAHAVETM
                     AEFQGLLLALEQETEPAELCS"
     gene            complement(2879..2967)
                     /locus_tag="VHE33_07600"
     tRNA            complement(2879..2967)
                     /locus_tag="VHE33_07600"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2931..2933),aa:Leu,seq:taa)
     gene            complement(2988..4940)
                     /locus_tag="VHE33_07605"
     CDS             complement(2988..4940)
                     /locus_tag="VHE33_07605"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HVT97359.1"
                     /translation="MKWPLPKRSWLALLAAVCLLAPAALAQVDATQWRTGLVKLLTGW
                     RAHEGDNAAWAAPDFDDSAWQTARLDDLGPAQPGWRWYRLHVKLAPGHDNLHLLIAGG
                     DGTYELYINGVRSPAAAIRRIAVTRPTEQVFEVPNDSDDLVLALRTYGSTNYTTWHLP
                     LFLSAAIGTPQGIANDQASFESGRLYAAVPAIAINLAVILAGIGAFALYRSQRTRAEY
                     RWLGFYLLLLGLSNLLLACSVNGVLSLAWNNLGADPLIYGFTIMQIEFTFSFAGRRVN
                     RLWRTYEVILLLPLLVAASLYLGTVSATVYLLVEALAILPAALLLPVLLLVWYRRGNR
                     EAGWLILPSLLPTATTALYDIGSASQFTGLGGLQFLVNPVYLGSIPLQPTDLANLLFV
                     LAIGVVMFFRFTRVSREQARTAAELTAAREIQRKLVPETLPEINGYHIEAAYFPADEV
                     GGDFYQVIDSHDGATLVVVGDVSGKGLKAAMTCTLTLGSLRALAGEALSPGAILTRLN
                     RHLFEMGHEGFVTCLCARIAPQGEVTVANAGHLPPYRNGEEMLLEPGVPLGLAPEALY
                     AERVFRLDHGDRLTLLSDGVVEARNPRGELFGFERTQAISAQPADAIAEAALRYGQAD
                     DITVLTLTRSPAAGLAAHAQSAAALG"
     gene            5111..>5916
                     /locus_tag="VHE33_07610"
     CDS             5111..>5916
                     /locus_tag="VHE33_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97360.1"
                     /translation="MRSAGVISLVVLSLAMAAPPGQAQDRTRITPYGTTQNAYTKRLI
                     LKDGSFQIVTKWEVKKDRVRYYSAERAEWEEVPRDLVDWPATEKWNKEHEPGAAPPAM
                     APPMGTHEEQQKEAAVIDAEAAAERADQAARMPLVAPGLHLPDQDGVFILDNFQSIPE
                     LVHLDQSTGNVNRDTNHNVLRAAIGSFSGAKEPVRINGQAARVRVHVDDPVLYVSLNT
                     GNSEEVAPEDALVVNTHDVPNKDDYSSPESHYAIVRVDVRPGERVVGAVRV"
BASE COUNT         1133 a         1685 c         1951 g         1147 t
ORIGIN      
        1 tctgatctat ttgctgcgcg aagggtatga agtctatgcc gcggacgccg acccccaggc
       61 agtggacaag gtgcgcagta tggcgcgcca gttggctccg cctctgccgg cgtcaaattt
      121 tcgcgttgaa gcggtggagc acctgtcgtt cgacgatggc tgcgccgacg tcgtcatcag
      181 cagcgcagtc ctccacttcg cacgggacga cgcgcacttc gagtccatgc ttctcggttc
      241 gtggcaagtg ctgaaacccg gtggcctctt cttctgccgg ctcgcatcct caatcgggat
      301 cgaggaccag gtggaacgaa tcgaagggcg gcgctacaaa ctacccgacg gttcgactcg
      361 gtatctggcc gacgaaggaa tgcttcgctc ctggaccgag cgactgggcg aactcgccga
      421 ccctctcaaa acgacggtcg tccagaatca acgctcgatg actacgtggg tcatccgaaa
      481 gagcccgtaa ctaattttcg ataacaagcc ggattctctg gccagtgccc agctgcttat
      541 cgacattttg ggattgcaga atttcctaaa tcatggcatc ttgtaacagc actctgaaga
      601 aaggacgccc gaatggcggt tctcgacaat aaatcgcgca accggcagta tcggccgact
      661 tatcggctga cctgcggggc gtcgcttcgt ccgcgttaac gcggctcttc gactaccccg
      721 cataacttag atcgctcacg tcgcagcact cgtgcgcact gccgcaccag cttctgctgc
      781 ctcgcggaat ctctttcgcg gttcttctga ctcgcggtgg gattcttcgc cgcagtgcgt
      841 cctgtgaagg gagaaggcta tgttgaacgc gcccggagta gttttgatcg acccgaagta
      901 tccccacaac gtgggggctg cgattcgtgc gtgttcctgc ttcggcgtgg atgcgctcgt
      961 gtggacaggc tcacggattc agctatcgca gtatgagcgt ttgccacgcg aagagcggat
     1021 gaaggggtac acgcgagtgc actttctcaa ccatcagcgc ccgttcgagt tgtttggcga
     1081 ctgcacaccc gtttgcgttg aggtgtacga gaacagcgaa ccgctcacgc tcttcgagca
     1141 tccggagaac gccgtgtaca tcttcggacc ggaggacggg agcgttcccc aggtgatccg
     1201 gcgtctatgc caccggttcg tgcacatccc tgcgcacttc tgcctgaacc tcagcgcagc
     1261 ggtcaatgtt ctcctggttc atcggctgat gacacgacaa ctcgcaggta aggagccgtt
     1321 gctgccactc agggaaatgt tgcacgaggt ccgcggcgat gtgccgacac cggcgatgga
     1381 tgcagtggga tgggacggta aatgacaaca ccataacctc cggctgtgag ccggttagaa
     1441 agcaatgcaa tgccagcgtt tttgaagcac attctggatg gtcagaacca atttgcttcc
     1501 ggcggcctgc tgctcatgat cctcggcagc atcggggtct ttctgcgttc tctgccgcag
     1561 cgcctctggt ggtggctcga ggcgcagatg acgatgatgg tcaccgtcaa ggatgacgac
     1621 gacgcgtttg actgggtgaa ggagtggttt ctcgagcagg cgttcctgag gcgcgttcgt
     1681 cgtgtcgatc tggacacgac tcttcgcgga gccgagcttg cgctgattcc tgcccctggc
     1741 cggcactggt tctggcgagc cggtcgcccg ttctgggtgt ggttctatcg cagcacggaa
     1801 agcaagggca cgagccagag acgcatggag tcactgacgt ttcagaccat cggtcgcgat
     1861 cagaagtttc tcaaggcatt tgtcgacgag gtcgtcgcct gccatcgccg caagcgcagg
     1921 tcggcatcgt atctctatct ctacgatgat gggtgggact acgtcgcggc ctattcgccg
     1981 cgttcgctcg actccgtgct gctgaagccc ggtgagaagg agcatctgat ggaggacctg
     2041 aagcgtttcc gagcctcacg cagccgctac gcgcggctcg gcgtgcccta ccatcgtggg
     2101 tatcttctct atggtccgcc gggcactggc aagacctcgc tggtctcggc gattggggcg
     2161 aggttcggta tgtcgatcta cgtcgtcaat ctgacggaga tgaatgatcg cacactgaaa
     2221 ggcgcgatga attcggttcc ggagaactcc atcatactgt tcgaagatat tgactgcatg
     2281 aaggcgagca atcgccggcc ggaactgtcg ccgccggcag cctcactgag cgggaccaca
     2341 atgcgagacg gcaacaatgc gccggatcgc ggcggcgtca cactatctgg gctgctgaac
     2401 gtgctcgacg gcttccacgc tccgtcgaac gtcgccttcg tgatgacgac gaatgccgtc
     2461 gaggctctgg atcctgcgct gttgcggcct ggacgcatcg attacaagct ctatcttggc
     2521 gaagcctccg actcgcagaa gtatgagctc tatcggcgct tctttcccga agctacggat
     2581 tttgaggcca gggagtttgt agaagcccac gctgtcgaga cgatggctga attccagggt
     2641 ctgctgctgg ccctggaaca ggagacggag cccgcagaac tgtgcagcta atccgattct
     2701 gaatctcgca atagcaaaat agcggtggcc cggatctgaa caatgtcatt cgcggttgct
     2761 cattctgcaa tggttccgac gagtctgggc tagccggacc aacgggcaac gccatgcatc
     2821 agtcaacacc cctaagaact taggccataa atactactgt ataaattatt gattttgttg
     2881 gtgcccggag ggggacttga acccccacaa cccttgcggg tctgcggatt ttaagtccgc
     2941 tgcgtctgcc gatttcgcca tccgggccct tgtttcatct tcgttcgcta tcccaatgct
     3001 gcggccgatt gcgcgtgagc tgcgagcccg gcggctggcg accgggtcag ggtgagcacg
     3061 gtgatatcgt ccgcctgtcc atatcgtagc gccgcttcgg cgatggcatc ggcgggctgg
     3121 gcgctgatgg cctgggttcg ctcgaagccg aagagctcgc cgcggggatt gcgggcctcg
     3181 accactccgt cggagagcag ggtgaggcgg tctccgtggt ccagccggaa tacgcgttca
     3241 gcatagagtg cttcgggggc caaacccaga ggaacgccgg gctcgagcag catctcttcg
     3301 ccattgcgat agggcggcag atggccggcg ttggcgacag tcacttcacc ctgcggggcg
     3361 atgcgtgcgc agaggcaggt gacaaagccc tcgtgaccca tttcgaaaag atggcggttg
     3421 agacgtgtga gaatcgcgcc ggggctgaga gcctcgccgg cgagggcgcg cagcgagccg
     3481 agcgtcaggg tacaggtcat ggcggccttg aggcccttgc cgctcacatc gccgacgact
     3541 acgagcgtgg cgccgtcgtg gctgtcgatg acctggtaga agtcgccgcc gacttcatcc
     3601 gcggggaaat acgcggcttc gatgtggtag ccattgattt cgggcaaggt ctcaggcacg
     3661 agcttgcgct gaatctcgcg tgccgcggtg agctcggcgg cggttcgcgc ctgttctcgg
     3721 ctcacgcgcg tgaagcggaa gaacatgacg acgccgatgg cgagcacgaa gagcaggttg
     3781 gccaaatcgg ttggctggag tgggatgctt ccgagataga cgggattgac gaggaactgc
     3841 agaccgccga ggccggtgaa ctgcgaggcc gagccgatgt cgtagagggc ggttgtggcg
     3901 gtgggcaaca gactgggcag gattagccag ccggcctcgc gattgccgcg gcgataccag
     3961 accagcagaa gcacgggcag gagaagggcg gcaggcagaa tcgccagggc ttcgaccagc
     4021 agatagacgg ttgcggagac agtgcccaga tacagcgagg ccgcgaccag caagggcagc
     4081 agcaggatta cttcgtaggt tcgccagagg cggttgacgc gccggccggc aaagctgaag
     4141 gtgaactcga tctgcatgat ggtgaagcca tagatcagcg gatcggcgcc gagattgttc
     4201 caggccagcg acaggacgcc gttgaccgaa caggcgagga gcaggttcga gaggcccaac
     4261 agcaagaggt agaaacccag ccagcggtac tccgcgcggg tgcgctggct gcgatagagg
     4321 gcgaaggcgc cgatgccggc gaggatgacg gcgaggttga tggcgatggc gggcacggcg
     4381 gcgtacagcc gtccgctctc gaaggacgcc tggtcattcg cgatgccctg cggtgttccg
     4441 atggccgcgg acagaaaaag gggcaggtgc caggtggtgt agttggtgct gccgtaggtt
     4501 cgcagggcga ggaccaaatc gtccgaatcg ttgggcacct caaagacctg ctcggttggg
     4561 cgggtgacgg cgatccgccg gatcgcggcc gccggcgatc ggacgccgtt gatgtagagc
     4621 tcgtaggtgc catcgccgcc ggcgatgagc agatgcaggt tgtcgtggcc gggggccagc
     4681 ttcacgtgga ggcggtacca gcgccatcct ggctgtgcgg ggccgaggtc gtcgaggcgg
     4741 gcggtctgcc aggcgctgtc gtcgaagtca ggcgcagccc atgcggcgtt gtcaccctcg
     4801 tgcgcccgcc agccggtgag cagcttgacc aggccggtgc gccactgggt ggcatccacc
     4861 tgggccaagg ccgccggagc gagaaggcac acggccgcga ggagcgcgag ccagctccgt
     4921 tttggcagcg gccacttcaa ggtaagctct cctcggggat gtcgcggttg cctggggtac
     4981 cccagtctat cgttcggcct tccgcgccat caagaaattc ggcgcggctg gcaggaaaga
     5041 tccatggcaa aagtctgggt tgcaggtttg ctggtgacgg atcggcgcag cccctatcta
     5101 atggagtgca atgcgatctg ccggtgtcat ctcgttggtg gttctgagtc ttgcgatggc
     5161 agcgccgcct gggcaagccc aggatcgcac gaggatcacc ccctacggca ccacgcagaa
     5221 tgcctacacg aagcggctca ttctgaagga cggctcgttt cagatcgtga ccaagtggga
     5281 ggtcaagaag gatcgggtcc gctattacag cgcggagcgc gcggagtggg aagaggttcc
     5341 cagggatctg gtggactggc cggcgactga aaaatggaac aaggagcatg agccgggcgc
     5401 ggcgccgccg gcgatggcgc ctccgatggg cacgcatgaa gagcagcaga aggaagcggc
     5461 cgtaattgat gcggaggccg ctgcggagcg ggccgatcag gccgcgcgca tgccgctcgt
     5521 cgctccggga cttcacttac ccgatcagga cggtgtgttt atcctcgata acttccagag
     5581 cattccggaa ctcgttcatc tggaccagag caccggcaat gtaaatcggg atacgaacca
     5641 caatgttttg cgcgctgcta tcggttcgtt cagcggcgcg aaggagcctg tccgcattaa
     5701 cggacaggcc gcgcgggttc gcgtgcatgt cgacgaccct gtactgtatg tctcgctcaa
     5761 taccggcaac tccgaggaag tggcaccaga ggacgccctg gtcgtgaaca cgcatgatgt
     5821 gccgaataag gacgattaca gttcaccgga gagccactac gcgatcgttc gcgtggatgt
     5881 tcgtcccggc gagcgagtgg ttggggcggt gcgcgt
//
LOCUS       DASZYN010000293        13046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1038131, whole genome shotgun sequence.
ACCESSION   DASZYN010000293 DASZYN010000000
VERSION     DASZYN010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 13046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13046
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038131"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VHE33_07615"
     CDS             complement(<1..501)
                     /locus_tag="VHE33_07615"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014873.4"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HVT97361.1"
                     /translation="MAENIGRVIQISGPAVDVQFEEATMPPIYEALRVVSEGFDVPVP
                     INVILEVQQHLGEGRVRCVAMEATDGMVRGMKAIDQGGPITVPVGRATLGRVLNVIGE
                     PVDELGPVNSAKRMPIHRPAPAFDEQSTHEEMFETGIKVIDLIQPFLKGGKIGLFGGA
                     GVGKTVI"
     gene            complement(518..1594)
                     /locus_tag="VHE33_07620"
     CDS             complement(518..1594)
                     /locus_tag="VHE33_07620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FoF1 ATP synthase subunit gamma"
                     /protein_id="HVT97362.1"
                     /translation="MANVLDLRRRIRSVRNTRQITKAMKMVSAARLRRAQDRTVSARP
                     YAAMLASVLESLKRRADIYDPETGEVRHPLLLTRPEKRILLVVVSGDKGFAGAFNANI
                     FKAAANFMAENRDKEIDVEAIGRKGRDLFRRRYPAAQFSESRDDHQRRERQRGSGIEV
                     LEAPSDPLERIGYEGVRRVAETIIDLYIHEEIDAAYLVYNEFKSVIQQRVVVERVLPI
                     LSLTVQEIERAEEPSIEERERAAEAALSAGVSLYPHEAEAAQKQLEDESKNFGTAEVD
                     YIYEQPPQDIFADVLPRYVTSQLYHAVLESIAAEHAARMTAMDSATNNASEMIDAYTL
                     QMNRVRQAAITKEIIEIVSGAAAI"
     gene            complement(1610..3229)
                     /gene="atpA"
                     /locus_tag="VHE33_07625"
     CDS             complement(1610..3229)
                     /gene="atpA"
                     /locus_tag="VHE33_07625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HVT97363.1"
                     /translation="MAQIKADEITALLKEQISNYDAKMHVDEVGTVITLGDGIARLHG
                     LDKVMSGELLEFPHGIAGLAMSLEEDQVGSVLLGDYTEVREGDQVKRTGKILSVPVGD
                     AMIGRVVNALGQPIDDKGPIQTSNTLPVERLAPGVIDRQGVREPMMTGLKAIDSMIPI
                     GRGQRELIIGDRQTGKTAVALDTIINNKTNDLICIYCAIGQKRSSIAQVVQTLEEYDA
                     MGHTIVVAASASEPAPMLYLAPYAACAIGEYFRDKGKHALVIYDDLSKHAIAYREISL
                     LLRRPPGREAYPGDVFYLHSRLLERASKLSDKMGGGSLTALPVIETQAGDVSAYIPTN
                     VISITDGQIFLETDLFNSGVRPAVNVGLSVSRVGFSAAIKAIKQVGSTLKLDLAQYRE
                     LAAFAQFGSDLDKVTQNQLNRGSRLTELLKQPQFHPLRAEQQVIILFSGTQGYLDDLR
                     VDQIRAFEDGLYPYLDSAQSQLLKDIATKKALDDDLRNRIHAALKEYKANFVAEHEGA
                     KLKQSSVQPLKADEKKKDQPKKAEQPVAAAH"
     gene            complement(3296..3835)
                     /gene="atpH"
                     /locus_tag="VHE33_07630"
     CDS             complement(3296..3835)
                     /gene="atpH"
                     /locus_tag="VHE33_07630"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HVT97364.1"
                     /translation="MASFVSRYARAFAEVVFESRLDAKAIQGQLADFAAAWHESRELR
                     EFLMDPSIPVEQKVALLDKLNAKLNMAHETRNFIAVLIRNGRIAGVDEVVTDFRREVN
                     QKLGIHEAKVISARKLDEPERRDLEQQIAGLTGGTVEAQYEEDSSLLGGAVVQVGSTV
                     YDGSVRGRLDRLREELISG"
     gene            complement(3836..4546)
                     /locus_tag="VHE33_07635"
     CDS             complement(3836..4546)
                     /locus_tag="VHE33_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HVT97365.1"
                     /translation="MKNPARILRVLLLCALLASPAVYTRGEQAPDNSPRAAASKGAGS
                     EHMDEPETNSDIEQYRHSSVVQAIAHMTGLKTETAAQIFEDFNSGVLLFAIGFMLWKF
                     LPKIFRKRSADLEKDLAAARLATEDANRRLAEVEARLLRLDSEIEKFRLQVEQEAVED
                     EKRIHTSLEAERQRIVASAEQEIAAAQSTAQRELKKFAADLAIDNAMRGIQLSSDTDR
                     ALIREFGKSLNRNSGGKA"
     gene            complement(4543..5004)
                     /locus_tag="VHE33_07640"
     CDS             complement(4543..5004)
                     /locus_tag="VHE33_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HVT97366.1"
                     /translation="MDELLKQLGDLALGAVPTMIFFILLVLAYRFILFGRLVKTRDER
                     RERTSGALEKSRQAVTQADVRTQEYEARLRAARAEVFRNREQRIQRLNAEKESALAAS
                     RLAAQQKVESAQAALNAQAVDARRQIEASANQLAAQVLAAVLPSATAGSSH"
     gene            complement(5279..6652)
                     /gene="hflX"
                     /locus_tag="VHE33_07645"
     CDS             complement(5279..6652)
                     /gene="hflX"
                     /locus_tag="VHE33_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HVT97367.1"
                     /translation="MPLNATGSTAERVLLVTVVLGSPRKALTRGAAQARDAALSAPGA
                     STLVPDEDSRLTEFRELVLSAGAVVAGDIVQHRAKADPATLIGSGKVEELSAIAASTQ
                     ADIAIVDHDLSPTQLRNLENALPCRVIDRTQLILDIFARHARTREGQLQVELAQLEYM
                     LPRLTGRGKAMSQLGGGIGTRGPGETQLETDRRRIQRRVLQLRGELEAVRRTRSQQRQ
                     RRESVPVPTVALVGYTNAGKSTLFNAITRAGVLESSRMFATLDPKLSAVQLPSRRKIL
                     LSDTVGFIRDLPHTLVTSFRATLEEVRKAEILVHVRDASSPMSDEHKAEVEKVLGELG
                     VLDKPRLEVLNKIDLLGEEERVALTKLGGRLVATSAKERIGLEALIDRIDEELVQDPI
                     LEQRLQVPQSEGDALAALEAGTVIRDREYQGNLVKMTVAGPASLLGRYRRFRLNARKS
                     TQKEQTG"
     gene            complement(6687..6992)
                     /gene="hfq"
                     /locus_tag="VHE33_07650"
     CDS             complement(6687..6992)
                     /gene="hfq"
                     /locus_tag="VHE33_07650"
                     /inference="COORDINATES: protein motif:HMM:NF001602.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HVT97368.1"
                     /translation="MDNKPAQNIQDTFLNTVRKDKTPITIYLVSGVKLTGKIRSFDKY
                     SVLLENNSQEQLIFKHAISTVVSTRSVLHGEHRAPHAAQAGPVAVTAPQAVQEASGS"
     gene            complement(7219..8340)
                     /locus_tag="VHE33_07655"
     CDS             complement(7219..8340)
                     /locus_tag="VHE33_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HVT97369.1"
                     /translation="MFYSSPFARRLFPGLIFGLLVLAGCGHLRPHTSQEYVYVWARGT
                     YLRDRVAAVSNRVAEVSNGQRLQVMEHGRRFLKVRTEKGEVGWIEEHGVIDQETYDKF
                     TQLVQDNAKTPVVGTAVLRDDYWLRDAPGRSSDRFYLLPESDKLQLLKRASVPRPQVA
                     QALPVPLEKPVSRTNGDAASVPPPANLEDYWLIRDSNGHVGWVRGRTLDVDVPDAIAG
                     LAEGQRIVAAYVLKKVNDPESTAPSGQVAEYVAFLSPWKDGLPYDFDQVRVFTWNTKK
                     HRYETAYRERNVAGYLPVAVTQASAENQGTPAFSFRVAAGDQAALDPQTGMVNPGDTV
                     TESFRLEGVLVHRDGAAPPRSIPAPAPTHRQRKPHHKHR"
     gene            complement(8488..12993)
                     /gene="gltB"
                     /locus_tag="VHE33_07660"
     CDS             complement(8488..12993)
                     /gene="gltB"
                     /locus_tag="VHE33_07660"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HVT97370.1"
                     /translation="MGTRNRVAPAVSTGDSRPRTLIDERFDHESCGVGFVATLDNQPS
                     HDILQKALTALARLAHRGAVAADGKSSDGIGIHTAIPRELLLASTGFTLHAAQPLAVG
                     MVFLPSETSSAAAAIRDLEQAITARGFSILGWREVPTRPEILGEIAASTLPVIRQILV
                     VDRTLGSVSWQDRDRRLYLARKQFERGGAEGYVCSLSSSSIIYKAMCDARLLPEFYPD
                     LADARFVTPFALFHQRYATNVAPSWDRAQPFRILGHNGEINTIWGNRARMTARAATLP
                     DELRPIYTPSGSDSTSLDEVIELLARNGRNVAEAVRMVMPPGDTGRHSAFLAYHGDCI
                     EPWDGPAAVAFTDGHIIGAALDRNGLRPCRFFVTEDHLVVAGSEAGLVDLDPEKIVHS
                     GRLGPGQMMLADLANHTFYEDEDLQALFDRSAPEYEKLLESATLESTAAVPAIDPAEL
                     NQLQLGFGYTKEDVNMILKPMAMDGKDAVWSMGDDTPIAPLARTPRPVYAFFRQRFAQ
                     VTNPPIDSLREACVIQLHTRLGPWPHVLNTRERLPGLSLKSPFLSLSQMESLHQRKHD
                     LAGDLPLAVLECVFNPGCNLLIALDDLCSKAIELVRGGAAVLLLTDRTCGEQSIPIPM
                     ALATGIVHHALIRAGERIKVGVAVEAGDCRDLHHAAVLMGMGAGAVCPWLALETARQM
                     NPEKGEANLLHAFDLGLAKIMSKMGISVVDSYRGAHLFDCLGLSREVVERCFEGTPVP
                     LGGIGFPELEAQIRELWSRAYSSDAGPEAPENGDGGPLVTIAATKDLPDYGWVRFRKA
                     DKSEPHGWQPQTVRLLQTITGATKAGASPAPDTAVAWQAFTTNATEKEPAVLRDLLEV
                     RAAGEALPVGEVEPAQNLYRRFIASAMSLGSLSPEAHQTITAAMNMLGGRSNTGEGGE
                     DPAVYEPGAQLPLVGNKVPSELLNNKVKQVASARFGVTAEYLMHADELEIKIAQGSKP
                     GEGGQLPGHKVTELIARLRHAQPGVQLISPPPHHDIYSIEDLAQLIYDLRRINPKAAI
                     GVKLVSECGVGTVAAGVAKAYADYIVIAGHNGGTGASPLSSIKYAGNPWELGLAETQQ
                     VLIRNGLRGRVRLRCDGGLRTARDIVIAALLGADEFAFGTAVLVALGCDMARQCHLNT
                     CPTGIATQKPELRAKFRGKPEQLVRFFEGLADEIRVLLGELGLPSLEAAIGRTDLLEQ
                     TRFDGCLDLTPMFGAPADGGKAGERHWQGKRNLRPEDHAPIDDAWVEPAVSAYKAGET
                     FQIDSLIANEDRTFGARLAGELAMLQSAGQMARAPLSFRLTGEAGQSFGAFAVSGMEL
                     ILKGLANDFVGKSLSGGEIILRGQGRAALQSELHTILGNVALYGATSGALFAAGRAGE
                     RFAVRNSGALAVVEGVGDHGCEYMTGGLVAVLGATGNNFGAGMTGGEAWVFDEDGLFL
                     SRQRYHSGFLTPEPWETLDAAGRERILGLVQLHGDKTASTRAFWLLSKWEELASRFVR
                     LTPKPQA"
BASE COUNT         2468 a         4262 c         3744 g         2572 t
ORIGIN      
        1 gatgaccgtc ttaccgacac cggcgccacc gaagagaccg attttgccgc ctttaaggaa
       61 gggctggatg agatcgatga ccttgatgcc ggtctcgaac atctcttcgt gagtcgactg
      121 ctcgtcgaag gcgggagcgg gacgatggat gggcatgcgc ttcgccgagt tcaccggacc
      181 cagttcatca accggctcgc cgataacatt cagcacgcgg cccagggtcg cgcgacccac
      241 tggaacggta ataggaccgc cctggtcgat cgctttcatc ccgcggacca taccgtcggt
      301 cgcttccatg gcgacgcagc ggacgcggcc ttcgcccagg tgctgctgca cctcaaggat
      361 gacgttgatg ggaaccggga cgtcgaagcc ttcgctgacc acgcgcaggg cctcgtagat
      421 ggggggcatg gtcgcttctt cgaactgcac gtccactgcg ggaccggaaa tctgaattac
      481 tctgccgatg ttttctgcca tgtctctctg ttctcgctta aatcgcggct gcgccgctga
      541 caatctcgat aatttccttg gtgatcgccg cctggcgaac gcggttcatt tgtaaggtgt
      601 aggcgtcgat catctctgaa gcattgttgg tcgccgaatc catggccgtc atgcgcgcgg
      661 catgctcggc ggcaatcgat tccaggactg cgtggtagag ctgcgaagtc acgtagcgcg
      721 ggagcacgtc tgcgaagata tcctgcggcg gctgctcgta gatgtagtcc acctctgcgg
      781 tgccaaagtt cttcgattcg tcctccagct gtttttgcgc ggcctcggcc tcgtggggat
      841 acaggctgac gccggccgac agtgccgctt ccgctgcccg ttcgcgctcc tcgatgcttg
      901 gttcctcggc ccgctcgatc tcctggacgg tcagggagag gattggcagg actcgctcca
      961 cgacgacacg ctgctggata acagatttga actcgttgta gaccaggtac gcagcgtcga
     1021 tctcttcgtg aatgtagaga tcaatgatcg tttcggcgac ccggcgaacc ccctcatagc
     1081 caattctttc cagcggatcg gagggcgctt cgagcacttc aatgccgctt ccccgctgcc
     1141 gttcccgacg ctggtggtcg tcccggctct cggagaactg agcggccgga tagcggcgcc
     1201 ggaagagatc ccgtcccttg cggccgatgg cctcgacgtc aatttccttg tcgcggtttt
     1261 cggccataaa gttggccgcg gccttgaaga tgttggcgtt gaacgcgccc gcaaatccct
     1321 tatcgccgga gacaacgacc agcaggatac gtttctccgg acgagtgagc agcagcgggt
     1381 gccgcacttc gccagtttcg ggatcgtaga tgtccgcgcg gcgcttgagc gattccagca
     1441 cgctggccag cattgccgca tagggacggg cggaaaccgt gcggtcctgc gcacggcgca
     1501 gacgggccgc cgaaaccatc ttcatggctt tggtgatctg ccgcgtattg cgcacgctgc
     1561 gaatgcggcg tcgtaaatcg agaacgttgg ccatttgctt cctgcgcgct tagtgagcgg
     1621 ccgccaccgg ctgctcggct ttcttcggct gatccttctt cttctcgtcc gccttcagcg
     1681 gttgcaccga actctgcttc agcttcgcgc cctcgtgttc ggccacaaaa ttcgccttgt
     1741 actccttgag tgcagcatga atccgattgc gaagatcgtc gtccagtgcc ttcttcgtgg
     1801 cgatgtcctt caacaattgc gactgcgccg aatccagata cggatacagc ccgtcttcga
     1861 acgcgcgaat ctgatcgacc cgaagatcgt cgagatagcc ctgagtaccg gagaacagga
     1921 tgatcacctg ctgctcggcg cgcagcggat ggaactgcgg ctgtttgagc aattctgtca
     1981 ggcgcgatcc gcgattcagc tggttctgtg tcaccttgtc cagatcactg ccgaactgcg
     2041 caaaggccgc cagttcgcgg tactgcgcca gatccagttt cagcgtcgag cccacctgct
     2101 tgatggcctt gatggcggcc gagaatccga cacgcgagac ggagaggccg acgttcacgg
     2161 ccggacgcac gccggagtta aacagatcgg tttcgaggaa gatctggccg tcggtgatcg
     2221 aaataacgtt ggtcggaatg tatgcggata cgtcgcctgc ctgcgtctcg atgacgggca
     2281 gcgcggtcag cgatccgccc cccatcttgt cgctcagctt cgaggcccgc tccagcaggc
     2341 gagagtggag atagaacacg tcgccagggt acgcttcgcg gccgggtggg cggcgcagca
     2401 gcaacgagat ctcgcgatag gcaatggcgt gcttggaaag atcgtcgtag atgaccagcg
     2461 catgcttgcc cttgtcgcgg aagtactcgc caatggcgca cgcggcgtag ggcgcgaggt
     2521 acagcatcgg agccggctcc gatgctgagg ccgccacgac gatggtgtgg cccatcgcgt
     2581 catactcttc cagcgtctgg accacctggg cgatcgagga gcgcttctgc ccgatcgcgc
     2641 agtagatgca gatcaggtca tttgtcttgt tgttgatgat cgtgtcgagc gcaaccgcgg
     2701 ttttgcccgt ctgtcggtcg ccgatgatca gctcacgctg tccgcgcccg atcggaatca
     2761 tcgagtcgat cgccttcagc cccgtcatca tcggttcacg cacgccctgg cggtcgataa
     2821 ccccgggagc aagacgctcg accggcagtg tgttgctggt ctggatcggt cccttgtcgt
     2881 cgatgggctg gccaagcgcg ttgaccacgc ggccgatcat ggcatcgccg accggcacac
     2941 tgagaatctt tccggtgcgc ttcacctgat cgccttcgcg aacctcggtg tagtcaccca
     3001 gaagcacgct gccgacctga tcttcctcca gactcatggc caggcccgca atcccgtgcg
     3061 ggaattccag caactcgccc gacatcacct tgtcgagccc atgcaggcga gcgatgccgt
     3121 cacccagcgt gatcacggtg ccgacttcgt cgacgtgcat cttcgcgtcg taattggaaa
     3181 tctgctcttt caggagcgct gtaatttcgt ctgctttaat ttgcgccatg cttgtgggtt
     3241 cctggttctg gattgattct cttttttctc ttccggcccg ctgccgcggc gagcgctaac
     3301 cggatatcag ttcttctctc agccggtcga gccggccgcg taccgacccg tcatacaccg
     3361 tgctccccac ctgcacgact gcgccgccca acagggagct gtcttcctcg tactgggcct
     3421 ccaccgttcc tccggtgagg cctgcaatct gttgctccag atctctgcgc tccggctcat
     3481 ccagcttcct ggcagagatc accttcgcct cgtgaatgcc cagcttttgg ttgacctcgc
     3541 ggcggaaatc cgtcacaact tcatccacgc ccgcgattcg gccattgcgg atcaataccg
     3601 cgatgaagtt tctggtttca tgagccatgt tcagcttcgc gttcagtttg tcgagcagcg
     3661 ccaccttctg ctccaccggg atcgatggat ccatcaggaa ctcgcgcaac tcccggctct
     3721 cgtgccaggc cgctgcgaag tccgccaact gtccctggat agctttggcg tcaagtctcg
     3781 actcgaaaac cacctcagcg aaggcgcgcg cgtagcgcga aacaaagctg gccatctatg
     3841 ccttgcctcc cgagttccgg ttgagcgact tgccgaattc gcggatcagc gcacggtcgg
     3901 tatccgagct cagctgaatc ccgcgcatcg cgttatcgat cgcgagatcg gcggcaaatt
     3961 tcttcaactc acgctgcgcg gtggactggg cggcggcaat ttcctgctcc gcggacgcga
     4021 cgatgcgctg ccgctcggcc tccagcgacg tgtgaatgcg cttctcgtcc tccacggctt
     4081 cctgctccac ctgcaggcga aacttttcga tttcggagtc gagtcgcagg aggcgagcct
     4141 cgacctccgc cagacggcgg ttcgcatcct cggtcgcgag ccttgccgca gccagatcct
     4201 tctccagatc cgctgaacgc ttccggaaga tcttcggcaa aaacttccag agcataaaac
     4261 caatcgcgaa cagcaaaact ccggagttga agtcctcgaa aatctgcgcg gctgtctcgg
     4321 tcttcagccc tgtcatgtgc gcgattgcct ggacgacaga ggaatggcgg tactgctcga
     4381 tgtcggagtt ggtctccggc tcgtccatgt gctcgctgcc tgcacccttc gaagccgcag
     4441 cccgcggcga attgtcaggc gcctgctcgc ctcgcgtata aaccgctggc gacgccaaca
     4501 gagcacacaa caacaggact ctcaaaatgc gggcaggatt tttcaatgcg aactccccgc
     4561 tgtcgccgag ggcagcaccg cggccagcac ctgcgccgcc agctggttgg cggacgcctc
     4621 aatctgcctc ctggcatcca cggcctgcgc attgagagca gcctgcgccg actccacctt
     4681 ctgctgcgcg gccaggcggg aggcggcaag ggcgctttcc ttctcggcgt tcagccgctg
     4741 gatgcgctgc tcgcgattcc gaaagacctc tgcgcgcgca gcccgcagcc gggcctcata
     4801 ttcctgggtt ctcacatcgg cctgagtaac cgcctggcgt gacttttcca gcgccccgga
     4861 tgtccgctcc cggcgctcgt cgcgggtctt gaccagccgg ccaaacaaga tgaaccggta
     4921 ggccaaaacc aacagaatga aaaagatcat ggtcggtacg gcaccgagcg caagatcacc
     4981 aagttgtttc agcaactcat ccatgaatgt ccggcggtag cagtcgggaa cgactgacga
     5041 ggcttaggtg aacctttggt tctaacagac gcaaaaaaac ggtgtcaaat gacgaaaggc
     5101 acttacgttt gcgcccattc ctcacgctcc gttccggcgt aaaccaagtg gcttggtcgc
     5161 ggtgagaatc gacgatttcc gacttgaccc ggcccggcaa cggcgtacac tgagaatacc
     5221 acctcgaccc ggatcagggt cggcgaaccg ggtgatcgga gatacccacc actctccatc
     5281 atccggtttg ctctttctgc gtggattttc gagcgttcag gcgaaagcgg cggtagcgtc
     5341 ccagtaaaga tgccggcccc gcgacggtca tcttcaccag gtttccctgg tactcacgat
     5401 cccggattac cgttcccgcc tcgagagccg ccagtgcgtc cccctccgac tgaggaacct
     5461 ggaggcgctg ctccagaatg gggtcctgca ccagttcctc atcgatccga tcgatcagcg
     5521 cctcgagccc gatgcgctcc ttcgccgatg ttgccaccaa ccggccgccc aacttcgtca
     5581 gtgcgacccg ctcctcttcg ccgagcaggt caatcttgtt caggacttcg aggcggggtt
     5641 tgtcgaggac tcccagttca ccgagcacct tctcgacttc cgccttgtgt tcgtcgctca
     5701 tcggactgga ggcgtcccgc acatggacca gaatctccgc cttccgcacc tcctccagcg
     5761 tggcgcgaaa cgatgtcacc agtgtatgcg gcaggtcgcg aatgaaaccg actgtgtcag
     5821 ataacagaat ctttctccgg ctcggaagct gaacggcgct gagttttggg tcgagagtag
     5881 cgaacatgcg cgacgattca agcactcccg cccgtgtgat cgcgttgaac agcgtgctct
     5941 ttcctgcgtt tgtgtagccg acaagagcca ccgtcggaac cggcaccgat tcacgacgct
     6001 ggcgctgctg cgaacgcgtg cggcgaaccg cttccagctc tccgcgcaat tgcagcacgc
     6061 gacgctgaat gcgacgtcga tccgtttcca gctgcgtttc accaggaccg cgggtgccga
     6121 tgccgccgcc gagctgcgac atggctttgc cccgccccgt aaggcgcgga agcatgtatt
     6181 ccagctgcgc cagttccacc tggagctgcc cttcgcgggt gcgcgcatgg cgggcgaaga
     6241 tgtccaaaat gagttgcgtc ctgtcgatca cccggcacgg cagcgcgttt tcaaggttgc
     6301 gtagctgtgt cgggctcaga tcgtgatcga cgatggcaat gtcggcttgc gtcgatgccg
     6361 cgatcgccga gagctcctct accttgccgc tgccgatcag cgtcgccgga tcggccttcg
     6421 cacgatgctg aacaatgtca ccagcgacga cagcgcccgc gctgagaacc agctcgcgaa
     6481 attctgtcag ccgggagtct tcgtcaggaa ccagagtgga ggcaccgggt gccgacaggg
     6541 ctgcgtcacg cgcctgagct gcacctcgag tcagagcctt ccgcggcgac ccaagaacaa
     6601 cagtcaccag cagaacccgt tccgcggtcg aaccggtcgc gttcagcggc atcgaaactc
     6661 gtcgcggatg tgatgctgcg cccggcttag gagccggacg cctcctgcac tgcctgcggt
     6721 gctgttaccg ccacaggacc agcctgtgcg gcatgggggg cgcgatgctc tccgtgcaga
     6781 accgagcgag tgctcaccac cgtggagatt gcgtgtttaa aaatcagctg ctcctggctg
     6841 ttgttttcca gcagcaccga atacttatcg aaggaacgga tcttcccggt gagcttcacg
     6901 ccgctcacca gataaatcgt gattggggtc ttgtctttgc gaacggtgtt caggaaggtg
     6961 tcctgaatgt tctgtgccgg cttattgtcc atgagccgat ctcctttgcc cttaattgaa
     7021 ctcgacggtg aaactgcaac agcagaaaaa ctgcagcggg cccaaaaaga aggtaacgcc
     7081 gcacccaccg tcaaggtgtg ttaccaagat acggggcagg cagccctttg cgcaacatca
     7141 ccggccggtt gcgcagctat ctttctaccc gttcactttg tgcccgacga taactaattc
     7201 gttatgaggg caaatcgctt aacggtgttt gtgatgaggt tttcgctggc gatgagtcgg
     7261 agccggagca gggatcgacc gcggcggggc ggctccatcc cgatgtacca gcactccttc
     7321 gagacgaaag ctctccgtca ccgtatcccc cggattcacc atgcctgttt gaggatcgag
     7381 tgcagcctga tcgcccgcag cgacgcggaa tgaaaaggcc ggagtgccct ggttttctgc
     7441 cgacgcctgg gtcactgcaa ctggaagata tcccgccaca ttgcgctcgc gataggcggt
     7501 ctcatagcga tgcttcttcg tgttccaggt aaaaacccga acctgatcga aatcgtacgg
     7561 aaggccatct ttccatggcg aaagaaatgc tacatactcg gccacctgcc cgctgggagc
     7621 ggttgactcc ggatcgttca ctttcttcag cacataagcc gcaacgattc tctgtccttc
     7681 ggccagtccc gcgatcgcgt cgggaacatc cacgtccagg gtcctcccgc ggacccaccc
     7741 aacgtgcccg ttcgagtcgc gaatcagcca atagtcctcc aaattcgcgg gcggcggcac
     7801 cgaagcagca tcgccattcg tccggctgac gggcttttcc agcggaaccg gcaaagcctg
     7861 cgccacctgc ggcctgggga ctgacgctcg cttcagcagc tgcagcttgt cgctttccgg
     7921 caacaagtaa aagcggtccg aactgcgtcc cggcgcatcc cgaagccagt aatcgtcgcg
     7981 caaaacagcc gttcccacta cgggagtctt cgcgttgtcc tgtaccagct gggtgaactt
     8041 gtcgtaggtt tcctggtcaa tgacgccgtg ctcctcaatc cagccgacct cgcccttttc
     8101 tgtcctgact ttcaaaaagc ggcggccgtg ctccatgacc tggaggcgct gcccgttgct
     8161 gacttcagcc acacgattgc tgactgccgc aacccggtcg cgcagataag tgccgcgtgc
     8221 ccaaacgtag acatattcct gggatgtgtg cggcctgagg tggccacagc ccgcgaggac
     8281 cagcaaccca aaaatgagcc cgggaaacag gcgacgcgca aagggagagg aataaaacat
     8341 ggagctggat gacagacccg tcttatggat tctgcaggct gggtcaagga tacatctgat
     8401 tcagaacacc agcccctgcg gagaagttgc gggagattac ttttgggatt cacccacgaa
     8461 gatcgtcgcg agccgcgtgg gttcccgcta tgcctgtggc ttgggtgtca ggcgcacaaa
     8521 ccgagacgcc agttcttccc atttcgagag cagccagaac gcgcgcgtgc tcgccgtttt
     8581 gtcgccgtga agctgcacca ggccaagaat cctctcccgg cccgcagcat ccagcgtctc
     8641 ccaaggctcc ggtgtcagaa acccactgtg ataacgctgc cgcgaaagga aaagcccgtc
     8701 ttcgtcgaag acccacgcct cgccgcccgt catgccggcg ccgaagttgt tcccggttgc
     8761 gcccagaacc gccacgagcc ccccagtcat gtactcgcac ccgtgatcgc cgacaccttc
     8821 cacgaccgcc aaagcacccg agttccggac cgcgaagcgt tcgccagctc gcccggccgc
     8881 aaacagtgcc ccactcgtcg cgccataaag agcgacgttg cccagaatgg tgtgcagttc
     8941 cgactgcagc gccgcccggc cttgtccgcg caggataatt tcgccgccgg agaggctttt
     9001 tccgacaaaa tcgttagcca gccctttaag gatcagctcc atcccgctga cagcaaaagc
     9061 gccaaacgat tggccggcct cgccagtaag gcggaagctg aggggagccc gcgccatttg
     9121 tccggccgat tgcagcatcg ccagttcgcc tgccagcctc gcgccaaacg tccgatcttc
     9181 gttcgcgatc agcgaatcga tctggaacgt ctcgccggcc ttataagccg acaccgccgg
     9241 ctcgacccat gcatcgtcaa tcggcgcatg atcttccggc cgcagattgc gcttgccctg
     9301 ccagtgacgt tcgcccgcct tgccgccatc cgccggcgca ccgaacatcg gggtcaggtc
     9361 cagacagcca tcgaagcgcg tctgctcgag caaatccgtc cgtccgatgg ctgcttcgag
     9421 cgaaggcaat ccgagttcgc ccagtaagac acgaatctcg tccgccagcc cctcaaagaa
     9481 gcgaaccagc tgctccggct ttccacggaa tttcgcccgc aactccggct tctgggtagc
     9541 gatcccggtc ggacaggtgt tgaggtggca ctgccgcgcc atgtcgcaac ccagcgcgac
     9601 cagcaccgcc gtcccaaaag caaattcatc cgctcccagc agagccgcaa tgacaatgtc
     9661 ccgcgccgtg cgcagtccgc cgtcgcaacg cagacgcacg cgtccgcgga gtccgtttcg
     9721 gatcagcact tgctgcgtct ccgcgaggcc cagttcccag ggattgcccg cgtatttgat
     9781 gctcgacagc ggggacgcgc ccgtgccgcc attatggccg gcaatcacaa tgtagtcggc
     9841 gtaggctttt gcgacccccg cggcgaccgt tccaacacca cactccgaaa ccagctttac
     9901 gccgatcgct gccttcggat tgatccgccg caggtcgtag atcaactgcg ccagatcttc
     9961 gattgaataa atatcgtggt gcggcggcgg actgatcaac tgtaccccgg gctgcgcatg
    10021 acgcaggcgc gcgatcagct cagtcacctt gtgtccaggc aattgtccgc cttcgccggg
    10081 tttcgatccc tgagcgatct tgatctccag ctcgtcggcg tgcatcaggt actcagcggt
    10141 caccccaaat cgcgccgagg ccacctgctt caccttgttg ttcagcagct ccgaaggtac
    10201 cttgttcccg accagcggca actgcgcccc tggctcgtag accgcggggt cttccccgcc
    10261 ctcacccgtg tttgagcggc cgcccagcat attcatcgcg gccgtgatgg tctggtgagc
    10321 ttcgggactc agtgaaccga gcgacatcgc actggcgatg aatcgccggt agagattctg
    10381 cgcaggctcc acttcgccaa ccggcaaagc ctctccggca gcccgaacct ccaacagatc
    10441 acgcaggacg gctggctctt tttccgttgc attcgtcgta aaggcctgcc acgcaaccgc
    10501 cgtgtctggc gcaggagagg cacccgcctt cgtcgccccg gtaatcgtct gcagcaagcg
    10561 cacagtctgc ggctgccacc catgcggctc ggatttgtcc gctttgcgga agcgtaccca
    10621 cccgtaatcg ggcaggtcct tcgtcgcagc aatggtgacc agcggtccac cgtctccatt
    10681 ttccggcgct tccggtccag catcgctgct gtacgctcgg ctccacagtt cgcgaatctg
    10741 tgcttccagc tccgggaagc cgatgccgcc caatggcacc ggcgttcctt caaagcagcg
    10801 ctcgacaact tccctgctca ggcccaggca atcgaacaga tgcgcaccgc ggtagctgtc
    10861 caccaccgag atgcccatct tcgacatgat cttcgccaac ccgaggtcaa acgcgtgcag
    10921 aagattcgcc tcgcccttct ccgggttcat ctgccgcgcc gtttccagcg ccagccacgg
    10981 acacactgcc ccggcgccca tgcccatcag cacggccgca tgatgcagat cgcggcaatc
    11041 cccagcctcg accgccacac caaccttaat ccgctcgccg gcgcgaatga gcgcgtgatg
    11101 cacaatcccc gtcgccagcg ccatcggaat gggaatgctc tgctccccgc aggttcgatc
    11161 cgtcagcaac agaaccgccg cgccgccccg caccagctca atcgccttgg agcaaagatc
    11221 gtccagcgcg atgagcaaat tgcatccggg attgaagaca cactccagca ctgcgagcgg
    11281 caggtccccc gccagatcgt gcttgcgctg gtggagtgac tccatctgcg acaacgagag
    11341 gaatggcgac ttcaacgaca gtccgggcag ccgttcgcgc gtgttcagaa catgcggcca
    11401 aggtccgaga cgcgtgtgca gctgaatcac acacgcctcc cgcagcgaat caatcggcgg
    11461 gttcgtcacc tgagcaaagc gctgccggaa gaacgcatac accggccgcg gcgtgcgcgc
    11521 cagaggtgcg atcggcgtat cgtctcccat cgaccagacg gcatccttgc cgtccatggc
    11581 catgggcttc aggatcatgt tgacgtcttc tttggtgtag ccgaatccca gctgcagctg
    11641 gttcagctcc gccggatcaa tagccgggac cgcggcggtc gactccagcg ttgcgctctc
    11701 cagcagcttt tcgtattccg gcgccgagcg atcgaagagc gcctgcagat cctcatcctc
    11761 atagaaggtg tggttggcga gatcggccaa catcatctgc cccggcccaa gccgtccgct
    11821 gtgcacgatt ttctccggat cgagatccac cagccccgct tccgagccgg caaccaccag
    11881 atgatcctct gtgacgaaaa atcgacaagg acgcagcccg ttgcgatcga gcgctgcacc
    11941 aatgatgtgc ccgtcggtga aagcaacagc agccggcccg tcccaaggct caatgcagtc
    12001 cccgtgataa gccagaaacg ccgagtgccg gcctgtgtcg cctggcggca tcaccattcg
    12061 caccgcctca gccacattac ggccattgcg ggccagcaac tcaatcacct cgtcgaggct
    12121 cgtcgaatcc gagccgctcg gcgtgtagat cggccgcagc tcatccggca aagtggcggc
    12181 gcgagccgtc atgcgcgcgc gattacccca gatcgtattg atttcgccgt tgtggccgag
    12241 gatgcggaac ggctgcgcgc gatcccacga tggcgccacg ttcgttgcgt agcgctgatg
    12301 gaagagggca aacggcgtga cgaagcgcgc atccgccagg tccggataaa actccggcag
    12361 cagccgggcg tcgcacatcg ccttataaat gatggaagaa gatgacaggg agcagacgta
    12421 gccctcggct ccgccacgct cgaattgttt gcgggccaga tacagacgcc ggtcgcggtc
    12481 ctgccaggag acagagccca gcgtccgatc aaccacgagg atctgccgga tcaccggcag
    12541 cgtcgaagcg gcgatttcgc ccagtatctc cggccgcgtc ggcacttcac gccagcccag
    12601 aatcgaaaac ccgcgcgccg taatggcctg ttccaggtcg cggatagctg cggcagccga
    12661 ggacgtctca gacggcagga agaccatgcc gacagccagc ggctgcgccg catggagggt
    12721 gaatcccgtg gaagcgagca gcagttcgcg ggggatcgcg gtatggatcc cgatcccatc
    12781 gctcgacttg ccgtcagcgg ccacggcgcc gcgatgcgcc aaacgcgcaa gcgcagtcag
    12841 cgccttctgc aaaatgtcat gcgagggctg gttgtcgagg gtagcgacaa atccgacgcc
    12901 acatgattca tgatcgaaac gttcgtcgat cagagtcctg ggccgggagt cgccggtcga
    12961 cacggccgga gcgacacgat ttctcgttcc catatccaac tataacggcc ttctctaacg
    13021 cccgaaagcg aatttagaaa tttctg
//
LOCUS       DASZYN010000294         2480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_974468, whole genome shotgun sequence.
ACCESSION   DASZYN010000294 DASZYN010000000
VERSION     DASZYN010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974468"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VHE33_07665"
     CDS             <1..235
                     /locus_tag="VHE33_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97371.1"
                     /translation="LCLPRPYKPKKLHLDSQLGMPTARKFAPGTTFVVYLAQQMRQNA
                     DQYTTTSFFSRVGAGEAGKYLSPIRRRVKKCGG"
     gene            237..755
                     /locus_tag="VHE33_07670"
     CDS             237..755
                     /locus_tag="VHE33_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97372.1"
                     /translation="MISERHPLRQLFHEVVAGCYHRYSGIDDPEVTSYVGDLLTEFTA
                     AENMVLRDPEGRPLRDLHAMIEASDPVFGAAASFDEERKLRRRIGDYALFATGMYPES
                     THLWKHTGDASFVEMVKTGKESYSIVAAFDLFEYRDEAPLFAQLSNEFERCMNGLKYV
                     REELDKMGGMIQ"
     gene            complement(874..>2480)
                     /locus_tag="VHE33_07675"
     CDS             complement(874..>2480)
                     /locus_tag="VHE33_07675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HVT97373.1"
                     /translation="SNPNPSAASPLAPAPGVMGGVPPASWVPSANRWMIAVSVMTATF
                     MVVLDSSVATVALPHIAGNLSASTDEATWVLTSYLVSNAIMLPATSWITGRIGRKRLL
                     LLSIILFTAASLLCGLAIDMPMLIVARVLQGIGGGGMLPLAQAILLESFPPREHGTAM
                     AVYGVGIVVAPVIGPTLGGWITDSYSWRWIFYINLPVGILALFLAGVYIEDPPYIRRK
                     LQGAIDYLGFALMALWLGSLQLVLDKGQESDWFGAVWIRWTAFLSIAAFVSFLVRELR
                     VHDPIVQLHVLRNRNFVTGTLITAAFGFVLYGVTAMLPLFLQTLLGYPALDSGLAVSP
                     RGIGSMLAMLVAGGLAVRLDGRVMLAGGLCIFGLSTLMLSHINLSIGIAAVAIPCVIN
                     GFGGGLTFVPLTTMAMGRLQKEEIGNASGIYNLMRNIGGSVGIAMVTTLLVRRGQVHQ
                     NYLAGHIRADAQTATNAAAGLAAHLAGSGFDAWDAHRRALGALYRMVEQQASLLAYAD
                     NFAMLGYLALFSMPLVLLFQRVRGSR"
BASE COUNT          550 a          820 c          688 g          422 t
ORIGIN      
        1 tctctgtctt ccccgtccgt ataaaccaaa gaaactacac ctggattcgc aattggggat
       61 gccgactgcc agaaagtttg ccccaggtac cacctttgtc gtttatttgg cgcaacaaat
      121 gcggcaaaat gccgatcaat atacaacaac aagcttcttc agccgggtgg gagcaggaga
      181 agcagggaag tacctttctc caatccggcg cagggtgaag aagtgcggag gctgacatga
      241 tttccgaacg ccatccattg cgccagttgt tccatgaagt cgttgcaggt tgctatcacc
      301 gctacagcgg gatcgacgat ccggaggtca cctcctatgt cggcgatctg ctcacggagt
      361 tcactgcagc cgagaacatg gttctgcgcg atccggaagg caggcctctg cgcgatctgc
      421 acgccatgat cgaagcgtcg gacccggtct tcggcgccgc cgcctccttt gacgaggagc
      481 gcaagctacg gcgtcgtatt ggcgactacg ccctgttcgc caccggcatg tatccggaat
      541 ccacgcatct ttggaagcac accggcgatg ccagcttcgt tgagatggtc aaaaccggca
      601 aggaaagcta ttccatcgtc gcggcgttcg atctgtttga gtatcgtgat gaagcgcccc
      661 tcttcgccca gctctcgaac gagttcgagc gctgcatgaa cggcctcaag tacgttcgcg
      721 aagaactcga caaaatgggc ggaatgattc agtaaccgat cgacaaacgt gaataaccag
      781 tagccagcag gaaatcgaca cgcatccagc ctgctggcca ctcgctactg gctattggct
      841 gttgcctact gcctactgcc tatttcctac tgcctaccgg ctgcccctga ccctctggaa
      901 caacaacacc agcggcatcg agaacagcgc gagatacccc aacatcgcaa aattgtccgc
      961 atatgccagc agagaagcct gctgctcgac catccgatac agtgcgccca gggccctccg
     1021 gtgcgcatcc cacgcgtcga atcccgaccc cgccaaatgc gccgccaaac ctgccgccgc
     1081 attcgtcgcc gtctgcgcat cggccctgat atgcccggcg agatagttct ggtggacctg
     1141 tcctcgccgc accagcagcg tggtcaccat ggcaatcccc accgatccgc caatgttccg
     1201 catcaggttg tagatgcccg aggcgtttcc gatctcctct ttctggagcc gccccatcgc
     1261 catggtcgtg agcggaacga aggtgagccc gcccccgaat ccgttaatga cgcagggtat
     1321 ggcgaccgcg gcaatgccga tgctcaggtt gatgtggctc agcatcagcg tcgagagccc
     1381 gaaaatacaa agccccccag ccagcatcac ccgcccgtcc agacgcacgg caagaccgcc
     1441 ggccaccagc atcgccagca tggagccgat gccgcgcggg ctgaccgcga ggccgctgtc
     1501 cagcgccggg taccccagca gtgtctgcag aaacagcggc agcatcgctg taacgccata
     1561 cagcacgaag ccgaaggccg ccgtgatcag cgtccccgtc acgaagttcc ggttccgcag
     1621 cacatgcagt tgcacaatcg ggtcgtgcac ccgcagctcg cgcaccagaa agctgacaaa
     1681 cgcggcgatg gagagaaacg ccgtccagcg aatccacacc gcgccgaacc aatccgactc
     1741 ctgccccttg tcgagcacca gctgcagcga tcccagccac aaagccatca gcgcaaaacc
     1801 cagataatcg attgcgccct gcagcttgcg ccggatgtac ggcggatcct cgatgtaaac
     1861 gcctgccaga aagagcgcaa ggattccgac cggcaggttg atgtagaaaa tccaccgcca
     1921 cgagtagctg tcggtgatcc agccgcccag cgtcggccca atcaccggcg ccaccacgat
     1981 ccccacgccg tacaccgcca tcgctgtgcc atgctcgcgg ggcggaaagc tctccagcag
     2041 gatcgcttgt gccaggggca gcatgcctcc gccgccaatg ccctgcagca cgcgcgccac
     2101 gatcagcatg ggcatgtcga tggcaaggcc gcagagcagg gaagccgcgg tgaacaagat
     2161 gattgacagc agcagcagcc gcttgcgtcc aatccttccc gtaatccagc ttgtcgccgg
     2221 aagcatgatc gcgttcgaca ccaggtagct ggtcagcacc catgtcgcct catccgtcga
     2281 agccgagagg ttgccggcga tatggggcaa cgccaccgtc gccaccgagg aatcgagcac
     2341 aaccatgaac gtcgccgtca tcaccgagac ggcgatcatc catcggttcg ccgatggaac
     2401 ccaggaggcg ggcgggactc cgcccatgac gcccggagct ggagccaggg gagatgccgc
     2461 agatggattc ggattcgaag
//
LOCUS       DASZYN010000295         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1233477, whole genome shotgun sequence.
ACCESSION   DASZYN010000295 DASZYN010000000
VERSION     DASZYN010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233477"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /gene="rplQ"
                     /locus_tag="VHE33_07680"
     CDS             <1..491
                     /gene="rplQ"
                     /locus_tag="VHE33_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HVT97374.1"
                     /translation="GTNMRHRNAGYKLGRNTSHRRALLRNLVTSILLEDRIETTVAKA
                     KAARPHVEKLITLGKKGDLHARRQALAFLQTGEAVTRLFDTVAPRYGDRKGGYLRIIR
                     SGFRQGDGGEKAFIELVDAEQVLDEKRQKRAEARAKRREEMQKAMAESGKGEEGGEAK
                     GE"
     gene            1104..1895
                     /locus_tag="VHE33_07685"
     CDS             1104..1895
                     /locus_tag="VHE33_07685"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HVT97375.1"
                     /translation="MPSDSNVVRRGLRKLRQQTPAGRAAKTAEEAEARAIQTRLLPAV
                     LPQVPGFEMATAWQPSDEVSGDYFDVFPLPRNQTALCIADVSGKGLAAATLASELRDA
                     VHQFAPAASSPAELCTQVNQALSRPGQTRYVTMFYGMLDPASRLLRYESAGHCLPLLV
                     RGDGSVWFPASFSGLMGLFSHWLYQTQEVHLQNGDCLLLITDGILQAENRKGEEFGYQ
                     RLIAQVEKGRTGSAEALGQQILAELTRFSGGKLEDDASLIVARVL"
BASE COUNT          449 a          756 c          671 g          365 t
ORIGIN      
        1 aaggaacgaa catgcgtcat cgcaatgcag gatacaaact gggacggaac accagccatc
       61 gccgcgcgct gctgcgcaac ctggtgacct ccattcttct cgaggatcgg atcgagacca
      121 cggtggccaa agccaaagcc gcgcggccgc atgtggagaa gctcatcact ctgggaaaaa
      181 agggcgacct gcatgcacgg cggcaggcgc tggcgtttct gcagaccggc gaggccgtta
      241 cccgcctgtt cgataccgtg gcgccgcgct atggcgatcg caagggcggt tatctgcgca
      301 tcatccgttc cggattccgc cagggcgacg gcggcgagaa agctttcatc gagctggtcg
      361 acgccgagca ggtcctcgac gaaaagcgcc agaagcgcgc cgaagctcgc gcgaagcgcc
      421 gtgaagaaat gcagaaagcc atggccgaaa gcggcaaggg cgaagaaggc ggagaagcga
      481 agggcgagta acaggcacgg ttcgtgcccc acatcggccc gctggcggca gatgtgggaa
      541 caagactctc catcacccgc ctctgtaaca gggcacgacc caaggggtgc cccccatctg
      601 cccggtgttg gcagatgtgg gaacaaggct ctcaatcagc cgcctgtaac agggcacgac
      661 cgaacgggtg ccccacatct gcccggtgtt ggcagatgtg ggaatgaaga tctcaatcca
      721 gccgtctgta acagggcacg acccaagggg tgccccccat cggcccgctg gcggcagatg
      781 tgggaacaag actctccatc agccgccttt gtaacagggc acgacttcag tcgtgccgat
      841 tagccctcaa atttctccgg gcttcagccc ctgagctttc cagcactcaa ggcgcatcct
      901 ccgcgggagc gcgcttttcc tgggccgggc tcgaaccgcc cgctcacgtt ccttgcgcaa
      961 catgtgccgc tccgcggcac gacggtagaa gccccggcct tcaggccggg gaaaacaggc
     1021 agcctccgag agggccttca ggccgggcac cgagcaaaat tttgccgcaa acgcgggctc
     1081 cggactgata cgcttcttcc cacatgccca gcgattcgaa cgtagtgcgg cgcggtctgc
     1141 gcaagctcag gcagcagact cccgcaggcc gtgccgcgaa aaccgccgaa gaggccgagg
     1201 cgcgcgcgat tcaaacacgc ctgctgcccg ccgtcctccc ccaggtccca gggtttgaaa
     1261 tggcgaccgc ctggcagccc tcggacgaag tcagcggaga ctacttcgac gtgtttcccc
     1321 tgccacgaaa ccagaccgcg ctctgcatcg ccgacgtatc gggcaagggt ctggccgccg
     1381 caaccctggc cagcgaactc cgcgacgccg tccaccaatt cgcccccgcc gcatcctcgc
     1441 ccgccgaact ctgcacccag gtcaatcagg cgctcagccg tcccggacaa acgcgctacg
     1501 tcacgatgtt ctacggaatg ctcgatcctg cctcccgcct cctgcgctac gagagcgcgg
     1561 gacattgcct ccccctgctg gtgcgcggcg acggcagcgt ttggtttcct gccagcttca
     1621 gcgggctcat gggcctgttt tcgcactggc tctatcagac ccaggaggtt catctccaga
     1681 acggcgactg cctcctgctc atcaccgacg gaattctcca ggcagagaat cgcaagggtg
     1741 aggagttcgg ctaccagcgc ctcatcgccc aggtggagaa ggggcggacc ggaagcgcgg
     1801 aggcgctcgg acagcagatc ctcgccgagc tgaccaggtt cagcggcggc aaactcgagg
     1861 acgacgcttc gctcattgtg gcgcgggtgc tgtaactcgc gatcagcttg cagccgcgtt
     1921 gaacgatcca tgattgcgcc ttcccggaat gagtctttgc gcaatccggt gaaaaggaaa
     1981 cctgccccgg cgccagcctc cagccatggt cgaatcgcag cggagatttt gtgcccagca
     2041 atcccggcgg cccgagcaat tcacgggatt gtaaccgccg ccctcacccg caacctttat
     2101 attttcaggc atgtccgctg aacctttgac gctggaaagc gtcgccggcc aggccccggg
     2161 aagccgcatc ctgcgcctca agggtcccct cgtgctcaac aacatgttcg agttccagag
     2221 cttcctgcgc aacgacccgc c
//
LOCUS       DASZYN010000296         2245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_393865, whole genome shotgun sequence.
ACCESSION   DASZYN010000296 DASZYN010000000
VERSION     DASZYN010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393865"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VHE33_07690"
     CDS             complement(<1..752)
                     /locus_tag="VHE33_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HVT97376.1"
                     /translation="MILVVAEQRDGKLNRVSFETIAGAQAIAKATGWTVDVVLPGSGI
                     AALAQELAGKAVAKVVALEADALKAYTSDAYVHALKAFIGEKQPKLVLFPHTYQVRDF
                     APRLALALDRTLISDATGYKVVGGAVQFTRQMFQGKFAADVSFGGEAPWMATIQIGAF
                     RGDQAEAGSAPVENVTAAVADTPARVTPHEVFQEAKQTVDLSQAEVIVAVGRGIKEQK
                     NMSLAENLAAALGGEVAASRPICDNGWLPLER"
     gene            complement(834..1112)
                     /locus_tag="VHE33_07695"
     CDS             complement(834..1112)
                     /locus_tag="VHE33_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97377.1"
                     /translation="MANLNIPEAERNLSPAQVAALDTRRRWGLVFQVIAGQFAFFAVL
                     LLLWTGQDLTYSPGWVHPMFYYNVLAGVMAVIFGLYGTYLRRGRIHEY"
     gene            complement(1282..2052)
                     /locus_tag="VHE33_07700"
     CDS             complement(1282..2052)
                     /locus_tag="VHE33_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HVT97378.1"
                     /translation="MKILVCIKQVPQKDAPLRLNETGTWIRDDVSWETNEPDAFALEE
                     ALRQKEKHGGEVAAITAGPARAGQVLREALAKGADRAIHLEDDRFAQMDAANTARAIA
                     AAIEGENFDLIVTGLQSDDFGAAQTGVLLAEILGIPHATIIIGIEKTDSGLKLKRELE
                     AGHYQHIDMTLPAVLTIQSGINKLRYATLIGIKQAKNKPLRKVAWAEVEGKLSKNVQK
                     IEKLYVPGSTKKTRKIEAPAGEAAKQVVEYLKNEARVL"
BASE COUNT          407 a          850 c          548 g          440 t
ORIGIN      
        1 tgccgctcca gcggcagcca tccgttgtcg cagataggcc gcgacgccgc gacctcaccc
       61 ccgagcgccg ccgcgaggtt ctccgccaac gacatattct tctgctcctt gatcccgcga
      121 cccaccgcga cgatcacctc cgcctgcgag aggtcgacgg tctgcttcgc ctcctggaag
      181 acctcatgcg gcgtcacacg cgccggcgta tctgccaccg ccgccgttac attttcaacc
      241 ggcgccgacc ccgcctccgc ctggtcgccg cgaaacgccc caatctggat cgtcgccatc
      301 cacggtgcct cgcctccgaa agagacgtcg gccgcaaact tcccctggaa catctgccgc
      361 gtgaactgca ccgccccgcc cacaaccttg taacccgttg catccgagat cagcgtccgg
      421 tccagcgcca gcgccagccg cggcgcaaag tcccgcacct gatacgtgtg cggaaacagc
      481 accagcttcg gctgcttctc cccaatgaac gccttcagcg catggacata cgcgtccgac
      541 gtgtacgcct tcagcgcgtc ggcctccagc gcaaccacct tcgccacggc tttgccggcc
      601 agttcctgtg ccagcgccgc gattccgctg cccggcagca ccacatctac ggtccagccc
      661 gtcgccttcg caatcgcctg cgcccccgcg atcgtctcaa aggatacccg gttcagtttt
      721 ccgtcccgct gctcagcaac aacaagaatc atgtccgcca tacgcaagtc aggggagccg
      781 cagctcccct ctgtctcccc aaactctaaa ctctaatccc taaactctgc ttctcagtac
      841 tcgtgaatcc gtccgcgccg cagatacgtc ccatacagac cgaaaatcac cgccatcact
      901 ccggccagca cgttgtagta gaacatcgga tgcacccagc cgggcgaata ggtcaggtcc
      961 tgccccgtcc acagcaacag caacacagcg aaaaacgcga actgccccgc aatcacctgg
     1021 aacaccagcc cccaacgccg ccgcgtatcg agcgcagcca cctgcgccgg gctcagattc
     1081 cgttccgcct cggggatgtt cagattcgcc atcgtcgctt ttctctcctt cagggtgcct
     1141 ggctgtcagg ttggggtcga tccggactcg cacaaccggg gaaacgtcag cttaccaaat
     1201 cccgcatgcc ccgcgcacgc cgccgggtct gccagtcttt ttttggcgct atccgctaaa
     1261 cgctgctgga ggctcgcgtc ctcaaaggac gcgcgcctca ttcttcagat actccacgac
     1321 ctgcttcgcc gcctcgcccg ccggagcctc gatcttgcgc gtcttcttcg tgcttcccgg
     1381 gacatacagc ttctcgatct tctgcacatt tttcgacagc ttgccctcga cctccgccca
     1441 cgctactttg cgcagcggct tgttcttcgc ctgcttgatc ccgatcagcg tcgcgtagcg
     1501 cagtttgttg atcccgctct ggatcgtcag caccgccggc agggtcatat caatgtgctg
     1561 ataatgcccc gcctccagtt cccgcttcag cttcaacccc gagtccgtct tctcgatccc
     1621 aataatgatg gtggcgtgcg gaatccccag gatctccgcc agcagcaccc ccgtctgggc
     1681 cgcgccaaag tcatcggact gcagcccggt cacgatcagg tcgaagttct cgccctcaat
     1741 cgccgcggca attgcacgcg ccgtgttcgc cgcatccatc tgcgcaaacc ggtcgtcctc
     1801 cagatgaatc gcccggtccg cgcccttggc cagagcctcg cgcagcacct gcccggcccg
     1861 cgccggcccc gctgtgatcg cggcgacctc tccaccatgc ttttctttct ggcgcagagc
     1921 ctcttccagc gcaaacgcgt ccggctcgtt ggtttcccac gagacgtcat cccgaatcca
     1981 cgtccctgtc tcattcagcc gcagcggcgc atccttctgc ggaacctgct tgatgcaaac
     2041 cagaatcttc acgaggcaaa gtataacgaa gcctctcgtc ctcgcgaaaa ccgtatactt
     2101 aatagagaac gctgcggctt gcccctgccc gttcaaagaa caaaatccgt cgcgagtctg
     2161 tgcgccaagc gcatgtgccg ggacggccag tccatcccaa atcccttgag agttgcataa
     2221 gctgcatgat ttctgtcagc aacgt
//
LOCUS       DASZYN010000297         8399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1105907, whole genome shotgun sequence.
ACCESSION   DASZYN010000297 DASZYN010000000
VERSION     DASZYN010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8399
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105907"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /gene="atpD"
                     /locus_tag="VHE33_07705"
     CDS             <1..1086
                     /gene="atpD"
                     /locus_tag="VHE33_07705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HVT97379.1"
                     /translation="PAPAFDEQSTHEEMFETGIKVIDLIQPFLKGGKIGLFGGAGVGK
                     TVIIQELINNVATKHGGFSVFAGVGERTREGNDLWHEMQESGVIDLKDFSKSKAALIY
                     GQMTEPPGARLRVALTGLTVAEFFRDEEGADTLLFIDNIFRFTQAGSEVSTLLGRMPS
                     AVGYQPNLATEMGELQERITSTKRGSVTSVQAVYVPADDLTDPAPATTFAHLDATTVL
                     SRPLSELGIYPAVDPLASTSRILAPRAVGQEHYDVAQGVKRILQRYKDLQDIIAILGI
                     DELSEEDKITVARARKVQKFLSQPFHVAEQFTGIPGKYCKIADTVRSFKEVIDGKHDD
                     VPEQAFYMRGTIEEVLEEAEKLKATTK"
     gene            1089..1505
                     /gene="atpC"
                     /locus_tag="VHE33_07710"
     CDS             1089..1505
                     /gene="atpC"
                     /locus_tag="VHE33_07710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HVT97380.1"
                     /translation="MADQLKVRLVTPDRILVDQTADAVEVPGKSGYFEVLYGHAPLLS
                     EIGAGEVRLHGGEGGDQRYVVARGFVEVLPERVTILAESAQKPEEIDTGAVQEQLQEG
                     EKLWNEAGDDAEKYAHANEVIAEAEAKLQSAGAGKH"
     gene            complement(1537..1869)
                     /locus_tag="VHE33_07715"
     CDS             complement(1537..1869)
                     /locus_tag="VHE33_07715"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HVT97381.1"
                     /translation="MASYMIQVSYTTAAVAAFVANPQDRSDVIRKAIESLGGKLHNIY
                     LAFGDYDVVGMFDMPDNVSAAAFALAIKAGGSCKAVKTTPLLSATDGVAVQKKAAGAN
                     YKSVVSKK"
     gene            complement(1981..2811)
                     /locus_tag="VHE33_07720"
     CDS             complement(1981..2811)
                     /locus_tag="VHE33_07720"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HVT97382.1"
                     /translation="MLLRLGYDMQFDIPEPVACVAQLRVHTSRSAGLREPDRVTVEPA
                     IAVEEYADSFGNLCTRFLAPKGTLRLSSDFLFDDSGLADPAQPEARAHAIAELPHEVL
                     RFLLPSRYCEVESLTPTAFDLFGNTVPGWQRVQAICNWVNAKVTFGYAFARPTKTALD
                     VFTERQGVCRDFQHLAITFCRAMGIPARYVAGYLGDIGVPPAPSPMDFSAWFEVYLEN
                     RWWTFDARHNVPRIGRVLMATGRDAADVAFFTSFGAAPLKSFLVISEEVKDEPRLKLA
                     "
     gene            2975..3739
                     /locus_tag="VHE33_07725"
     CDS             2975..3739
                     /locus_tag="VHE33_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97383.1"
                     /translation="MTYCLGIMTQEGLVMASDSRTNAGFDDLNIARKMHTFSQPGERA
                     FVVLSSGSLSLSQSVVNLARARFGSGDGLARVGSMYDAARELGDCVRQVAELDRAALE
                     RDNYSFNVHLLLGGQVRGEEPALYMIYPQGNPLCATEESPFLQLGEYKYGRPLLDRGV
                     VFGSTSLMVAAKYALLSFDATMRSNGAIGPPIELMLYTRNSLTLDCYRKFTQGDSELQ
                     TIHARWEQSLRRAVEDLPEIHFASSPVEDSPQALLI"
     gene            3789..5201
                     /locus_tag="VHE33_07730"
     CDS             3789..5201
                     /locus_tag="VHE33_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HVT97384.1"
                     /translation="MSLPVESQPERGFWSSIREALRGSHQDFTTGSLNRSILLLAIPM
                     VLEMVLESLFAVVDTFWVGRLGASAVATIGLTESILALVFAIGLGVSLSTTAMVARRI
                     GEKDPEDAAISAVQAILLGLLISAGLGIPAGIEAPHLLRLMGASPAIVTVGSGYARIA
                     LGGCGAIIMLFLNNAIFRGAGDAAIAMRLLWVSNIINLILDPCLIFGLGPFPHLGVTG
                     AALATFTGRSIGVLYQFYRLMRGTERIQILTRHIYLNVPVLWRLLRVSLTGILQFAIA
                     NASWIGLVRIISLFGDAAVAGYTVAIRIVIFFILPAWGLSNAAATLVGQNLGARRPDR
                     AEQAVWRTGLYNMLFLALLGAFFIVFATPVVRLFVSDPAVIAIGARALRTFACGNIAY
                     AWVMVLLQAFNGAGDTVTPTFVNFFGFWVLEIPLAWWMATRLQLRAEGAFLSVVIAEA
                     SIAVISLILFKQGRWKKQRI"
     gene            complement(5207..7306)
                     /locus_tag="VHE33_07735"
     CDS             complement(5207..7306)
                     /locus_tag="VHE33_07735"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HVT97385.1"
                     /translation="MLRYALCMLKISGCTLLAAVCLGSFPLGHAIAEDHFPSNEDMRH
                     VRSMAQPKVSPDGQQVLIQIADSTADGGKTHLWLVDVKTNTSRQLTWSPASDKRGESQ
                     GQWMPDGGSILFVAHRGEHTQLFRLPMNGGEAHAFEVKIAPLVDESKEADAIPPAKKD
                     SAEKKDAKPEPLEADVARYFIAPDGKHIALIVRDPQTPGEKKQHDDKADAVWVNHDPH
                     GSRLYLLDPADGKLTPTAVPPDVQRVSWAQQSDKLVAITEEMNEASDLQPTGKAWLVA
                     TANPEHPSQIKAAPATIGAAVWSDDASHIDFLAQSKADAPPGYEDLYELTMADGSIRD
                     LTDGFDGSTGHEEPIAEAGAVFEPVEMGFHTTLMRFAGDQRQPLHFDRTTVSQFNTNA
                     KHTAWVWLGSSSDKPMELFYADKPGDAGRTLNTPDMLPAKWPEVKSRPLSWKSDNLTI
                     EGLLYLPAQAAKGKVPLIVDVHGGPTGAFEDNWNALTEFLLGQGWAVLRSNPRGSTGY
                     GAAFAAANKNDMGGGDYRDIMAGVDMVLAKYPIDDRKMALMGYSYGGEMAGFVEGRTD
                     RFKAIISGAPVIDQESEYGTEAGSYYDRWFYGGYPWEHAESAWRQSPLATVGKAKTPF
                     LLLQGESDKTDPLGQSQEMYRALRQMGVPVEMVQYPREDHGPLAGGIFGAPSPEPWHG
                     FDGRQRIVTFIKKAFGE"
     gene            7350..8081
                     /locus_tag="VHE33_07740"
     CDS             7350..8081
                     /locus_tag="VHE33_07740"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HVT97386.1"
                     /translation="MLHLLNPIPADEIRLIVFDLDGTLIDSRRDLTNSINAMLTEFGR
                     DPLPEPIIATYIGDGAGMLVRRALGDPEDEPLVERALQHFLAHYREHKLDYTYVYSGV
                     FDSLEALRTRAGGDPRKMAVLTNKPVRPSVDICDALGLSPFMFRIYGGNSFATKKPEP
                     EGLNAIIHEAGVAPGETLMIGDSSVDILTARRAGAWVIGCRFGLSSHTVEDIPADCLV
                     DSAGEWAQALSVESKAQCGANSSRC"
BASE COUNT         1536 a         2667 c         2447 g         1749 t
ORIGIN      
        1 cccgctcccg ccttcgacga gcagtcgact cacgaagaga tgttcgagac cggcatcaag
       61 gtcatcgatc tcatccagcc cttccttaaa ggcggcaaaa tcggtctctt cggtggcgcc
      121 ggtgtcggta agacggtcat catccaggaa ctcatcaaca acgtcgccac caagcacggc
      181 ggcttctctg tcttcgccgg cgtcggcgag cgcacccgcg agggcaatga cctctggcac
      241 gaaatgcagg agtctggcgt catcgacctg aaggacttca gcaagtcgaa ggcagctctt
      301 atctacggcc agatgaccga gccgcccgga gctcgtctcc gcgtggcgct cactggtctt
      361 accgtcgccg agttcttccg tgacgaagaa ggcgccgaca cgctgctctt catcgacaac
      421 atcttccgct ttacccaggc aggctctgag gtgtcgacgc tgctgggccg catgccctcc
      481 gctgtcggtt accagccgaa cctggccacc gaaatgggag aactgcagga gcgcatcacc
      541 tcgaccaaga ggggatccgt cacttcggtg caggctgttt atgtgcccgc cgacgacctc
      601 accgatccag cgccggcgac aacgtttgct cacctcgatg ccacgacggt gctctcgcgt
      661 cccctgtcgg aactcggtat ttatccggcc gtcgatccgc tggcctccac atcgcgcatc
      721 ctggcgccgc gcgccgtcgg gcaggaacac tacgatgttg cccagggcgt gaagcgcatc
      781 ctccaacgct ataaggacct gcaggacatc atcgccatcc ttggtatcga cgagctttcg
      841 gaagaggaca agatcactgt ggcccgcgcc cgcaaggtgc aaaagttcct ctcgcagccc
      901 ttccatgtgg ccgagcagtt caccggcatt cccggcaagt actgcaagat cgcggatacg
      961 gtccgcagct tcaaggaagt catcgacggc aagcatgacg acgtgccgga gcaggcgttc
     1021 tacatgcgcg ggaccatcga ggaagtgctg gaagaggccg agaaactgaa ggcgaccacg
     1081 aaatagctat ggctgaccag ctcaaagttc ggcttgtgac tcccgaccgt attctggtcg
     1141 atcagactgc ggatgccgtc gaagtgcccg ggaaaagcgg ttacttcgag gtgctctatg
     1201 gccatgctcc actgctgtct gagattggag cgggagaggt tcgtctgcac ggaggcgaag
     1261 gcggcgatca acgctatgta gtcgcgcgcg ggtttgtcga agtcctgccc gagcgtgtga
     1321 cgattctggc ggaaagcgcg caaaagcccg aagagattga taccggcgcc gtgcaggagc
     1381 aactgcagga aggcgagaag ttgtggaacg aggcggggga cgatgcggag aaatacgccc
     1441 acgccaatga agtcatcgcc gaagccgaag ccaaactcca aagcgccggg gcagggaagc
     1501 attaaccctg cccgcacgcc gcccgatcgc ccaggtctac ttctttgaga ctaccgattt
     1561 gtagttagca cccgccgcct ttttctgcac ggcaacgccg tccgttgcgc tgagcagcgg
     1621 agtggtcttg acagccttgc aggagccgcc cgccttgatg gccaacgcga acgcggccgc
     1681 gctgacgttg tcgggcatat cgaacatgcc gacgacatcg taatctccaa acgcgagata
     1741 gatgttgtgg agttttccgc ccagcgattc gattgctttg cgaattacat cgctgcggtc
     1801 ctggggattg gcaacgaagg cggcaacagc cgcggtcgtg tacgacacct ggatcatgta
     1861 gctggccatg ggtaaccctt tctcggattg atcttgctgc tggagcaggg aagcgattgg
     1921 gaggggcggc cagtatacct cggccgatca gcgattcgct cccgcaccgt ttttacaggg
     1981 ctacgccagt tttagccggg gctcgtcctt cacttcttcc gagatgacca ggaagctttt
     2041 cagcggtgcg gctccgaagg atgtgaagaa cgccacatcg gccgcatcac gaccggtcgc
     2101 catcagaaca cgcccgatcc gcggcacatt atgccgtgcg tcgaaagtcc accagcggtt
     2161 ctccaggtac acctcgaacc aggcgctgaa atccattgga gacggcgccg gtggaacccc
     2221 aatgtccccc aggtagccgg ccacgtagcg cgccggaatc cccatcgccc ggcaaaatgt
     2281 gatggccaga tgctggaagt cccggcacac gccctgccgc tcggtaaata catcgagcgc
     2341 cgtctttgtc ggacgagcga acgcgtagcc gaaggtcacc ttggcgttta cccaattaca
     2401 aatcgcctgc acgcgctgcc agcccgggac ggtgttccca aacagatcga acgctgtcgg
     2461 agtgaggctt tcgacctcgc agtagcggct gggcagcagg aaacgaagca cctcatgcgg
     2521 aagctcggca atcgcatgag cccgcgcctc gggctgcgcc gggtccgcca accctgaatc
     2581 gtcaaacagg aaatcgctgc ttagccgcag cgtgcccttc ggcgccagaa atcgcgtgca
     2641 gagattgccg aacgagtccg catattcttc gacggcaatc gcgggttcca cggtgacgcg
     2701 atccggttcc cgcagtcctg cggagcggga tgtgtgcacg cgcagctggg ccacgcaggc
     2761 caccggttcc gggatgtcga actgcatgtc ataacccagg cgaaggagca tcgggcctcg
     2821 tggtaccgtc cgcacgccgc cgattgggcg gcgcggtgat cttaagatgt agatgcctgc
     2881 cccggatgtt ggtcgaatcc ctttgattca agggggaaag ctgtcaaaat gacctgaaca
     2941 gcacctacgt ctccctggga gcagccccca ctcaatgacc tactgtctgg gcatcatgac
     3001 acaggaagga ctcgtcatgg catcggattc gcgcaccaat gccggattcg acgacctgaa
     3061 tatcgcacgc aagatgcaca ccttctcgca gccgggtgag cgcgccttcg tcgtgctgag
     3121 cagcggcagc ctttccctca gccagtctgt ggtgaacctg gcacgcgccc gctttgggtc
     3181 gggcgacggt ctggcgcgcg taggcagcat gtacgatgcc gcccgcgaac tgggagactg
     3241 cgtccggcag gtggcagagc tggatcgtgc cgcgctggag cgcgacaact actccttcaa
     3301 cgtgcatctg ctgctcggcg gtcaggtccg cggcgaggag cccgcgcttt atatgatcta
     3361 tccccagggc aatcccctct gcgcgactga ggagtcgccg tttctgcaac tgggcgaata
     3421 caagtacggg cgcccgcttc tggatcgcgg tgtagtcttc ggctccacca gtcttatggt
     3481 cgcggccaaa tatgctttgc tctccttcga cgccaccatg cgttccaatg gcgcgattgg
     3541 tccacccatc gagctgatgt tgtatacgcg aaacagcctc accctggact gctaccgcaa
     3601 gttcacgcag ggtgattccg aactgcaaac catccacgca cgctgggagc agtcgttgcg
     3661 ccgtgccgtt gaagatctcc ctgaaattca ctttgccagt tcgcctgtgg aagacagccc
     3721 acaggcattg ctgatctagc tgttcctctc acgattcctg ccgggccatg cgcaccgtgg
     3781 cgcaccctat gagcctccct gtcgaatcgc aacccgaacg cggtttctgg tcgtcgatcc
     3841 gtgaagccct gcgcggttcg catcaggact ttacgaccgg cagcctgaac cggtccattc
     3901 ttctcctggc gattcccatg gtgctggaga tggtgctcga atcgctcttt gcggtcgtgg
     3961 acaccttctg ggtgggcagg ctgggtgcca gcgccgtcgc caccatcggc ctgaccgagt
     4021 caattctggc ccttgtcttc gccattggcc tcggcgtctc gctgtcgacc acggcgatgg
     4081 tcgcccgccg gatcggcgag aaagatcccg aagacgcggc catctccgca gttcaggcca
     4141 tcctgctcgg actcctcatc tccgccgggc tcggcatacc cgccggcatt gaagctccgc
     4201 acttgctccg tcttatgggc gcgtcgcccg ccatcgtcac tgtgggctcc ggctatgccc
     4261 gcatcgcact cggcggctgc ggcgctatca tcatgctctt cctgaacaac gccatttttc
     4321 gcggagctgg cgatgccgcc atcgccatgc gcctgctttg ggtttcgaac atcatcaacc
     4381 ttatcctcga tccgtgcctc atcttcggtc tcggaccgtt cccgcacctc ggggtcaccg
     4441 gcgcagctct ggccacattc accggccgca gcatcggcgt cttgtatcag ttctaccggc
     4501 tcatgcgcgg gaccgagcgc atccagattc tcacccgcca catctacctc aacgttcctg
     4561 tgctgtggag gttgttgcgc gtttccctca ccggcatcct gcagtttgcg attgccaatg
     4621 ccagctggat cggcctcgtg cgcatcatct cgctcttcgg cgacgcagcc gttgcgggat
     4681 acaccgtcgc cattcgcatc gtgatcttct tcatcctccc ggcctggggt ctcagcaacg
     4741 ccgccgccac gctggtcggc cagaacctcg gcgcgcgccg tcccgatcgc gccgagcaag
     4801 ccgtgtggcg aactggcctc tacaacatgc tcttccttgc cctcctcggc gcattcttca
     4861 tcgtctttgc gacgcccgtt gtgcgtctct tcgtcagcga ccctgcggtg atcgcgatcg
     4921 gcgcacgtgc tctgcgcacc tttgcctgcg gcaatatcgc ctatgcgtgg gtcatggtcc
     4981 tgctccaggc tttcaatggc gcgggggata ccgttacgcc caccttcgtg aacttcttcg
     5041 gcttctgggt tctcgaaatc cccctcgcat ggtggatggc gacgcgcctt cagcttcgtg
     5101 ccgaaggcgc ctttctttct gtcgtgatcg cagaagcttc catcgctgtg atcagtctta
     5161 tcctcttcaa acagggccga tggaagaagc agcgaatttg aaatagctat tcgccgaacg
     5221 cttttttgat aaaggtcacg atcctctgcc tcccgtcgaa gccatgccac ggttccgggc
     5281 tgggagcccc gaagatcccg ccggcgagcg ggccgtgatc ttcacgcgga tactgcacca
     5341 tctccaccgg tacgcccatt tgtcgcagcg cgcgatacat ttcctggctc tgcccgagcg
     5401 ggtcggtttt gtcgctctcg ccctgcagca gcaggaaggg tgtctttgcc tttcccacgg
     5461 tcgctagcgg gctctgccgc catgcgctct ctgcgtgctc ccagggatag ccgccgtaga
     5521 accaccggtc gtaatacgat cccgcctcgg ttccgtattc gctctcctga tcgatgaccg
     5581 gagcgccgct gatgatggcc ttgaagcgat cggtgcgacc ttcgacgaag ccagccatct
     5641 cacccccata gctgtagccc atgagcgcca ttttgcggtc gtcgatcggg tacttcgcca
     5701 ggaccatatc gacaccggcc atgatgtcgc gatagtctcc gccgcccata tcgtttttgt
     5761 ttgctgcggc gaaggccgcg ccgtagccag tgctgcctcg gggattgctg cgcaacacgg
     5821 cccacccctg acccagcaga aattccgtca gggcattcca gttgtcctcg aacgcgcccg
     5881 tcggcccgcc gtgcacatcg acaatcagcg gaaccttgcc cttcgctgcc tgggccggca
     5941 aataaagcaa tccttcaatg gttagattgt cgctcttcca tgaaagcggg cgcgacttca
     6001 cttccggcca cttcgccggc agcatgtcgg gtgtgttcag cgtgcgtcct gcatcacccg
     6061 gtttgtctgc atagaaaagc tccatcggtt tatcgctgga ggacccaagc cacacccacg
     6121 ctgtgtgctt cgcgttggtg ttgaactgcg acaccgtcgt cctatcgaaa tgcaatggct
     6181 gacgctgatc acccgcgaat cgcatcaggg tggtgtgaaa gcccatttcc acgggctcga
     6241 agacggcacc cgcttccgca attggctcct catggccggt gctgccgtcg aagccatcgg
     6301 tgagatcgcg aatcgaacca tcggccatcg tcagctcgta caggtcttcg tagcctggag
     6361 gagcatcggc tttgctctgc gcgaggaagt caatgtgact cgcatcgtcg gaccacactg
     6421 cggctccgat ggtggcaggc gccgctttga tctgcgatgg atgctcggga ttcgcggtcg
     6481 cgaccaacca cgctttgcct gtcggttgca gatcgctggc ttcattcatt tcctctgtga
     6541 tcgcgaccag tttgtcactc tgctgcgccc acgaaaccct ctgcacgtcg ggcggaactg
     6601 ctgtgggcgt gagcttcccg tcagccggat cgagcagata cagacgggat ccatgcggat
     6661 catgattcac ccataccgcg tcggctttat catcgtgctg ctttttctcg ccgggcgtct
     6721 ggggatcccg aacgatgagc gcgatgtgtt ttccatccgg cgcgatgaag tagcgggcca
     6781 cgtcggcctc caggggttct ggcttggcgt ctttcttttc cgctgaatcc tttttcgcgg
     6841 gtgggatagc atccgcttcc ttcgactcat ccacaagtgg ggcgattttc acctcgaacg
     6901 catgcgcctc gccgccattc atcggcaggc gaaacaattg agtgtgctca ccgcgatgcg
     6961 cgacaaacag gatgctcccg ccatcgggca tccactgtcc ctgcgactcg ccgcgcttgt
     7021 cactggctgg cgaccatgtc agctggcgcg aggtattggt ctttacatcc acgagccaga
     7081 gatgcgtttt gcccccgtcg gctgttgagt ccgcgatctg aattaaaacc tgctgtccat
     7141 cgggcgagac ctttggctgg gccatcgagc gtacgtggcg catgtcctcg ttggaaggga
     7201 agtgatcttc agcgatcgcg tgcccgaggg gaaacgaacc gaggcaaacc gctgccagga
     7261 gcgtgcatcc tgagattttc agcatgcaca gagcatatcg cagcactcag ggaagttcct
     7321 gtgtccagcc gtcacattag actgagatca tgctgcatct gctcaatccg attccggccg
     7381 atgagattcg tctgattgtg tttgatctgg atggaacgct gatcgattcg cgccgggacc
     7441 tcacgaactc catcaacgcc atgctcactg agtttgggcg cgacccgctc cccgagccca
     7501 tcatcgctac ctatattggg gacggagccg gcatgctggt gcgccgcgcg ctgggtgatc
     7561 ctgaagacga gccgctggtc gaaagggctt tgcagcattt tctggctcat taccgcgaac
     7621 acaagctgga ttacacctat gtatattccg gggtgttcga ttcgctggag gcgctgcgca
     7681 ctcgcgctgg cggtgatccg cgcaagatgg ccgtcctaac caacaagcct gtgcggcctt
     7741 cggtggacat ctgcgacgcg ctgggcctga gcccgttcat gttccgcatc tacggcggca
     7801 atagctttgc caccaagaag cctgagccgg aaggcctgaa cgcgatcatt cacgaggccg
     7861 gcgtggctcc tggcgagacg ctcatgatcg gcgactccag tgtcgacatt ctcaccgcgc
     7921 ggcgcgctgg cgcatgggtg attggctgcc ggttcggact ttcctcgcac actgtcgagg
     7981 acattcccgc tgactgcctt gtcgattccg cgggcgaatg ggcgcaggcg ctctcggtcg
     8041 aaagcaaggc gcaatgcggg gccaattcct cgcgctgttg agcgcgagtt ccatcgcgca
     8101 acgcttctgg gttgccctcg cggctgcgaa ttttcacgca aaaacgcgcg gaccccgtcg
     8161 ggacagcgcg catcccatga actgaacgag gcacctccca cgatgtgcaa aggagagatg
     8221 tgcaaaggag agaaccatga gaatcgcaaa acacgcgacc gctctcgtac tgggtttggc
     8281 gttatgtggc ggcactgcgc tggctgttca tgcgcaggag ggctcgccct ggcaacctgt
     8341 cgaacctacg ggaagctggt cgcatgcatg gcacagtggt ttccacgatg gcattgaag
//
LOCUS       DASZYN010000298         9399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_269252, whole genome shotgun sequence.
ACCESSION   DASZYN010000298 DASZYN010000000
VERSION     DASZYN010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 9399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9399
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269252"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2202)
                     /locus_tag="VHE33_07745"
     CDS             complement(<1..2202)
                     /locus_tag="VHE33_07745"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF005122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HVT97387.1"
                     /translation="MADTRKSAPLAQTSTSVQDAGHPTTPTSATASGTKALRFARTFS
                     RPNASPYDEVQWERRTAAITDAGGKTIFEQKDVEVPVDWSMTATNIVASKYLHGQLGT
                     PERETGVRQLIGRVAETIRDWGLAGGYFASAEDAALFHDELAHLLVTQKVAFNSPVWF
                     NVGCDRLEPNSDAQNWHWNQHTCAVEFSVTGYRKPQCSACFINAVEDSLDSILTLAKT
                     EGMLFKWGSGTGTNLSTIRGSAETLSGGGTASGPLSFMRGFDAFAGVIKSGGKTRRAA
                     KMVILNVDHPDIVDFIECKSKEEAKAWALMREGYDGSSGPDSEAYSSIFFQNANNSVR
                     VTDEFMRAVEGDADFSTRTVKDGTPVKTYKARDIMTKIAEATYQCGDPGMQYDSTINR
                     WHTSKNTARINASNPCSEYMFLDNSACNLASFNLLKFVTPAGTFDIPAYRAAIDIMIT
                     AMEILVDNSGYPTEAIARNSHDYRPLGLGYANLGALLMAFGLPYDSDAGRDFAATLTA
                     IMCGEAYLQSARIAEACPPLASATPLTASVAHEGGACPGFYVNREPFLDVIRMHRAEV
                     NNIGKSKTAGDKTFAVPQLDGLISASKDCWDEALRHGEKYGYRNSQVTVLAPTGTIGF
                     MMDCDTTGIEPDLALVKYKKLVGGGMIKIVNNTVPTALFKLGYNNDQVDAIVSYIDAT
                     GTIEGAPGLKPEHLAVFDCSFKPAKGTRSIHYMGHVRMMAATQPFLSGAISK"
     gene            3284..4009
                     /locus_tag="VHE33_07750"
     CDS             3284..4009
                     /locus_tag="VHE33_07750"
                     /inference="COORDINATES: protein motif:HMM:NF016925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HVT97388.1"
                     /translation="MFFHRKDLMVPVEVRKPDPIYAQFLLQQFGGATGELTAGLQYWI
                     QSFHCPKPGIREMLQDIAVEEFGHLELVGKLIEEHTREIEKTAPEVQHNPIFAIRGRG
                     PHLVDDAGVSWTAAYINEGGDVVRDLRADIAAEAGARETYEVLLERAPDPGTKRVILY
                     LLTREVAHTKMFMKALESLGKLTEPLFGNVKPDETVKVYYHLSSNGQAQRGPWNQEPE
                     FTYVADPVKMIEHHELAGVGGKR"
     gene            complement(4161..6878)
                     /locus_tag="VHE33_07755"
     CDS             complement(4161..6878)
                     /locus_tag="VHE33_07755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT97389.1"
                     /translation="MISRLRSLLKNLFRKQQLDTDLDEELSAYLDLVSVEKMKSGLSP
                     EEARRAARREMGGAEQIRQGVRDIRAGVSLERLSQDIRYGLRTLRKNPAFSLVAVATL
                     ALGIGANTAMFSLLDQIVLRLLPVQQPERLMKVTIRGNNYGNTYGADRISWPMFEDLR
                     DRNQVFTDMFCRFPITVTVGYQDRAQQVMGELISGSYFPLLGVGAALGRTIAPDDDKV
                     PDSQPVVVLSYSFWRNYFNADRSIVGRTIELNGHSMTVIGVAQRGFDGVELGNPAQMF
                     IPIMMKTEMTPHSDGLKDRRRRLAWVGAYGRLKPGITAEQAQSSLQPLLHSILQMEVQ
                     QPDFRSFTNDDRLRFLRNRIEVVPGSDNGLRDTMQRPLWILIALTAAVLLLACASLAN
                     LLLARATVREREMAVRLAIGAARGRIVRQLMVETLLLSGAGVLLGLALAFLADHLLVL
                     TFLPANQATEFAVSPVPDIRVLAFTLGMLLVTSLAFGLMPALRGSRTAIAPSLQERSG
                     SGSAASVPLRRLFVVAQVALSLLLLVGAGLFVRTLRNLRNLGPGFPTRHLIEFTLDPS
                     LNGYSNDQTESFYERLNTNLQTMPGVSAVGLSSMPILKGYAWQNAIIGQESGAPQREQ
                     PVLSQIGPGYFSTLGIPILEGREFTDQDRGPQMVAVINQSFAKRYLPPGDPMGRRFGL
                     VDDQKPDLRPNIQVIGVIPDTKYRDLRETPPPQAYFSYLQGTNFRFMTVYLRTQGDPR
                     DLENALRERMRRFDPRVPLVDLQTVDEEIGWSLTTERLIASLSTVFGALAMLLAVIGL
                     YGVMAYTVTRRTREMGIRIALGATRSRIIGMVMREASFMVLVGLATGAGLALALTSLI
                     RNQLFGLNPHDPWTLFGAAFSLAVAAGLAGFIPALRASSVDPTNALRRE"
     gene            complement(6875..7219)
                     /locus_tag="VHE33_07760"
     CDS             complement(6875..7219)
                     /locus_tag="VHE33_07760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVT97390.1"
                     /translation="MAGPQSDLLQGTLDLLILKALALEQLHGMGISRRIAQMTNAAFD
                     VKAGSLFPALHRMEQAGWLTSSWGQAETNRRAKFYALTKAGRKQLQTETARWERISLA
                     MGAALGIRQEAL"
     gene            7634..7864
                     /locus_tag="VHE33_07765"
     CDS             7634..7864
                     /locus_tag="VHE33_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97391.1"
                     /translation="MRSNLVYSAGLKIENRFLLATVVMRAVRKLHIDSTRTEDTTNKV
                     LGQVAEGKFVEVELPAIVPPPAVDVLLIGSAA"
     gene            complement(8029..>9399)
                     /locus_tag="VHE33_07770"
     CDS             complement(8029..>9399)
                     /locus_tag="VHE33_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97392.1"
                     /translation="DRGDWMSPAHRTWMAVALSWTLLSLPASSSAQPTANAASSESLP
                     LRVSFYSGAAYPSMKPAASIEVFLNGTTGDLATREKFVAVIRSAEDHGWNCASPSSYA
                     VTVSGSTKRIPVSAVMPVGHENCNDRPTPAPVLLLLREKVDTNTKYVVSLEGLPDAAL
                     TGLSPGANLVSAAQAFPASTSYSVSVLPQAAPGEALTNGTTRDVGQLSVSFGVPYVGH
                     SPVFINTNDLFSTDAKDTMSSFAVTAGSQWGLLSAWYTPVQVSETLQGNQTASSLSAV
                     SNLSLSGLVPWYWTRQGLNNSWIDAPLAPEFALAAQYTHRFAQEVTAATPRLSGDDFS
                     VNPALAIEPFYVLPGLCSRYRKWIGAKAGDSTSRQFCVGSRIDLGLWYLPRDKTQAGS
                     QRVEGYGDASIVIPLSNLNFEKFQLIQKDNLLNSELTIKYSDSVNAANNYARSRQWTF
                     GISVIK"
BASE COUNT         1843 a         2526 c         3278 g         1752 t
ORIGIN      
        1 tttggagatg gctcccgaga ggaacggctg ggttgcggcc atcatgcgaa cgtggcccat
       61 gtaatgaatg gaacgggtgc ccttggcggg cttgaagctg cagtcgaaca cggcaaggtg
      121 ctcaggcttg aggccgggcg cgccttcgat ggtgccggtg gcgtcgatgt agctgacgat
      181 ggcgtcgacc tgatcgttgt tgtagccgag cttgaagagc gcggtgggga cggtgttgtt
      241 gacgatcttg atcatgccgc cgccgaccag cttcttgtac ttcacgaggg caaggtcggg
      301 ctcgattccg gtggtgtcgc agtccatcat gaaaccgatg gtgccggtgg gagcaagcac
      361 ggtgacctgg gagttgcgat agccgtactt ttcgccgtgc cgaagcgcct cgtcccagca
      421 gtctttggaa gcgctgatga gaccgtcgag ctgcggaacg gcaaaggttt tgtcgcccgc
      481 ggttttgctc ttaccgatgt tgttgacctc ggcgcgatgc atgcggatga cgtcgaggaa
      541 aggttcgcgg ttgacgtaga agccggggca ggcgccgcct tcgtgcgcga cggaggcggt
      601 gagcggcgtg gcggaagcca gcggcgggca ggcttcggca atgcgggcgg actgcagata
      661 agcctcgccg cacatgatgg cggtgagggt ggcggcaaag tcgcgtccgg cgtcggagtc
      721 atacggcaag ccgaaagcca tgagcagggc gccgaggttg gcatagccga ggccgagggg
      781 ccggtagtcg tgggaattgc gggcgatggc ttcggtggga tagccggagt tatcgacgag
      841 gatctccatc gcggtgatca tgatgtcgat ggccgcgcga tacgccggga tatcgaaggt
      901 gccggcggga gtcacaaact tgagtaagtt gaagctggca aggttgcagg cggagttatc
      961 gaggaacatg tactccgagc agggattcga cgcattgatg cgcgccgtgt tcttggaggt
     1021 gtgccagcgg ttgatggtgg agtcgtactg catgcccgga tcgccgcact ggtaggtcgc
     1081 ttcggcgatt ttggtcatga tgtcgcgggc tttgtaagtc ttcaccggtg tgccgtcttt
     1141 cacggtgcgg gtggagaagt cggcgtctcc ctcgacggcg cgcatgaact cgtcggtgac
     1201 gcgcacggag ttgttggcgt tctggaagaa gatgctgctg taggcctcgc tgtccggtcc
     1261 tgaagatccg tcgtagcctt cccgcatcag ggcccaggct ttggcttctt ctttggactt
     1321 gcactcgatg aagtcgacga tgtcgggatg gtcgacgttg aggatgacca tcttggcggc
     1381 gcggcgggtt ttgccgccgg atttgatgac gccggcgaag gcgtcgaagc cgcgcatgaa
     1441 gctcagggga ccgctggcgg tgccgccgcc ggagagggtc tcggccgagc cgcgaatcgt
     1501 ggagaggttg gtgccggtgc ccgagcccca cttgaagagc atgccctcgg ttttggcgag
     1561 ggtgaggatg gagtcgagcg aatcctcgac ggcattgatg aagcaggccg agcactgcgg
     1621 cttgcggtag ccggtaacgg aaaactcgac ggcgcaggtg tgctggttcc agtgccagtt
     1681 ctgcgcgtcg gaattgggct cgaggcggtc gcagccgacg ttgaaccaga ccggcgagtt
     1741 gaaggcgacc ttctgcgtca cgagcaggtg ggcgagctcg tcgtggaaga gggcggcgtc
     1801 ttcggcgctg gcgaagtatc cgccggccag gccccagtcg cggatggttt cagccacgcg
     1861 gccgatgagc tggcgaacgc cggtttcgcg ctcgggcgtg ccgagctgcc cgtggagata
     1921 tttgctggcg acgatgttgg tcgcggtcat ggaccagtcg acggggacct cgacgtcctt
     1981 ctgctcaaag atggtcttgc cgccggcgtc ggtgatagcg gcggtgcggc gctcccactg
     2041 cacctcgtcg tacggagagg cgttgggtcg ggagaaagtg cgcgcgaagc gcagagcctt
     2101 tgttccggaa gccgtagcgg aggtgggggt ggtgggatgg ccggcgtcct gaacggacgt
     2161 cgaagtttga gccaggggtg cggatttcct ggtatcggcc atgggtaatt cctctctggt
     2221 agtgatgttg cgcctgatgc gctgattctg cctgggtact acggttctgg ctggccctgg
     2281 gccggagcga cgaggctccg ggaaaactgg acgtcaccgc aaaggaaaaa gggcaaaaag
     2341 gccccccgga tccgatcgcg gcaggacaaa aattcgaaac catggcggat cagaaaccgc
     2401 aggaactcag gcgagggtgc ttcctgatgg gggaggcgac gaccgctttt tcttgagaac
     2461 ctcttacggg ttacttagag ccgcagatgg gaaagtacct ctgttaatgg ggtgtgtcaa
     2521 gcgcaaacca ctacatctgg tattttacgc gcagttgggg gaatctactc gcataattcc
     2581 cggaattcac ttgtgcagaa tcgcggaaag gcgcatgcgg cgcgggtttg aagcgcgaat
     2641 caaaacgcgg ccgggacgaa gggtgagggt ccggtggcac gcttcgcccc ggccggatct
     2701 gaggattttt gcatgtgcca cggattgagg ctacgcgcca gggtggggtg ggacaagatg
     2761 cgggaatggt gcagtttcgg tggaaaggtg tgggaggggt ggaaaacagg gactcgccgt
     2821 ccgggcggac gcgcttcgcg ccttcagcca gggttcagca ttccgcatcc gccctcgacc
     2881 atccctcgat ggtggtcccg gacaggcagc gaggcgcaac gctgtagccg ctgtaaggca
     2941 gcgctttttc gatgcggtgg aagtttgcac cgcgatgacg cgagcggtcg acagcaacag
     3001 gactagactg cggacgctga atgaagcgag cctgccgctg atttctcttt cgggcccctg
     3061 aagcttccgt gaggtcgatg caccaatgcg cgggcccgcg gctgacacct gcgctccggt
     3121 gatgcgcctg ggacgtccgg gcacgagttg aggagcgcgc acgattcaca cgaatcatcc
     3181 gggtcctcga cccccttcgg gctgcttgcg aagcgtggca actccacgtt ctgcttccgg
     3241 gtcgatcctc ggattccggg ccatagcagg aaggagattg cttatgtttt tccatcgcaa
     3301 agatctgatg gttccggttg aagtgcggaa gccggatccg atttatgcgc agtttctgct
     3361 gcagcagttt ggcggagcaa cgggcgaact gaccgcgggc ctgcagtact ggattcagtc
     3421 gttccactgc cccaagccag gaattcgcga gatgctgcag gacatcgcag tggaggagtt
     3481 cgggcacctc gaactcgtcg gcaagctgat tgaggagcac acccgggaga tcgaaaagac
     3541 tgcgcccgag gtgcagcaca acccgatctt cgcgattcgc ggccgcggac cgcatctggt
     3601 cgatgacgct ggcgtgagct ggacggcggc gtacatcaat gaaggcggcg atgtggtgcg
     3661 cgacctgcgc gcggatatcg cagccgaagc cggcgcgcgt gagacctatg aggtgctgct
     3721 ggagagggct ccggatccgg gcacaaagcg agtgatcctg tatttgctga ctcgcgaagt
     3781 ggcgcacacg aagatgttca tgaaggcgct ggagtcgctg ggcaagctga ccgagccgct
     3841 gttcggcaat gtcaagcccg acgagacggt gaaggtgtac taccacctgt cgtcgaatgg
     3901 gcaggcgcag cgcggcccgt ggaaccagga accggagttc acgtatgtgg ccgatccggt
     3961 gaagatgatc gagcatcacg agttggctgg agtgggcggc aagcggtaag tgccggcccg
     4021 aaagcctgtg tcaacggcga cccatcttca gtgagcagga ggatcactga agatggggcg
     4081 ctgtacgtct gggacaaatg aaaaattagg ggaaacggcg aggccagcgt atgcgagcgt
     4141 gcgaactcac ttgtaaaaca tcattcccgg cgcagagcat tcgtcggatc caccgagctg
     4201 gcgcgcagag ctgggataaa cccggcaagt ccagcggcga cagcgagtga gaaggccgcg
     4261 ccgaacagcg tccaggggtc gtgagggttg agtccgaaga gctgattgcg gatgaggctc
     4321 gtcagggcga gcgcgagccc ggctccagtg gcgaggccga ccagaaccat gaaggacgcc
     4381 tcccgcatga ccatgccgat gatcctggat cgcgttgcgc caagcgcgat gcggatgccc
     4441 atctcgcggg tgcggcgcgt gacggtgtac gccatcacac catagaggcc gatgaccgcg
     4501 agcagcatgg ccagtgcgcc aaaaacggtg gagaggctcg cgatcaggcg ctcggtggtg
     4561 agggaccacc cgatctcctc gtcgacggtc tgcagatcca cgaggggaac gcgggggtca
     4621 aaccggcgca tgcgttcgcg aagcgcattt tcgagatccc ggggatcgcc ctgcgtgcgc
     4681 aggtaaaccg tcatgaagcg gaaattcgtg ccctgcaaat acgagaagta agcctgcggc
     4741 ggaggagtct cgcgcaggtc gcgatacttc gtatcgggga taacgccgat gacctggatg
     4801 ttgggtcgga gatcgggctt ctgatcatcg accagtccga agcgccgtcc cattggatct
     4861 ccgggcggga gatagcgctt cgcaaaactt tggttgatga ctgcgaccat ctgcgggccg
     4921 cggtcctggt cggtgaactc gcggccttcg aggatgggga tgccgagcgt ggagaaatag
     4981 ccggggccga tctggctgag cacaggctgc tcgcgctgcg gcgctccgga ttcctgtccg
     5041 atgatggcat tttgccaggc atagcccttg aggatcggca tcgacgacaa gccaacggca
     5101 gagacaccgg gcatcgtctg aaggttggtg ttcaaccgct cgtaaaagga ttcggtttga
     5161 tcgttggaat agccattcag cgacgggtcg agggtgaact cgatcaggtg acgggtgggg
     5221 aaacccgggc cgaggttgcg gaggttgcgc agggtgcgca cgaagagacc ggctccgacg
     5281 agcaacagca gcgacagagc cacctgagcg acgacgaaga ggcgacgcag cggcacactg
     5341 gcggccgatc cggagccgga cctttcctga agcgacggcg caatcgcggt gcgtgaaccc
     5401 cgaagagccg gcatgaggcc aaaggcgaga gaggtgacga gcaacatgcc cagggtgaaa
     5461 gcgaggacgc gaatatccgg aacaggtgag acagcaaatt ccgtggcttg attcgcgggc
     5521 agaaacgtca gaaccaggag atggtcggca aggaaagcca gcgccaggcc gagaagcacg
     5581 ccggcgcccg agagcagcag cgtttccacc atgagctgcc gaacgatgcg tccgcgggcg
     5641 gcgccgatgg cgaggcgaac cgccatctct cgttcgcgca ccgtggcgcg cgccagcagg
     5701 agattggcaa gactggcgca ggccagcaga aggacggctg cggtgagcgc gatgagaatc
     5761 cagagcggac gctgcatcgt atcgcgcagg ccgttgtcgg aaccgggcac cacctcgatg
     5821 cgattgcgga ggaatcgcag gcgatcgtcg ttggtaaacg agcggaagtc gggctgctga
     5881 acctccatct gaagaatgct gtgcagtaaa ggctgaagcg agctttgcgc ctgctccgca
     5941 gtgatcccgg gcttgagccg gccgtaagcg ccgacccagg cgagacggcg gcggcgatcc
     6001 ttcaggccgt cggagtgcgg cgtcatctcc gtcttcatca tgatggggat gaacatctgc
     6061 gcagggttgc ccagctcgac tccatcgaag ccgcgttgtg cgacgccgat gaccgtcatg
     6121 ctgtggccgt tgagctcaat cgttcgaccg acgatcgacc ggtcggcgtt gaagtagttt
     6181 cgccagaagc tgtagctgag aacgacgact ggctggctgt cggggacttt gtcgtcgtcg
     6241 ggggcgatgg tgcggcccag cgccgccccc acgccaagaa gcggaaaata ggaaccggaa
     6301 atgagttcgc ccatcacctg ctgcgcgcga tcctggtacc caacggtaac ggtgatggga
     6361 aagcggcaga acatatcggt gaaaacctgg ttgcgatcgc ggaggtcctc gaacatcggc
     6421 cacgagatgc ggtcggctcc gtaggtattg ccatagttgt tgccgcggat cgtgaccttc
     6481 atcaggcgct caggctgttg gacgggaagg agtcgcagca cgatctgatc gaggaggctg
     6541 aacatggcgg tgttggcgcc gatgcccagg gccagcgtgg cgacagcaac aagggaaaag
     6601 gcgggattct tccggagggt tcgcaggccg tagcggatat cctgggagag cctctccagt
     6661 gacacgccgg cacggatgtc gcgaaccccc tggcgaatct gctcggcgcc acccatttca
     6721 cgacgggcgg cacgccgggc ttcttcgggg gacagacccg atttcatctt ttctacagaa
     6781 accagatcca gatacgcgct gagttcttca tcgaggtcgg tgtcgagctg ctgtttgcga
     6841 aacaggtttt tcaaaagcga gcgcagccgg ctgatcatag cgcctcctgg cggatgccca
     6901 gcgccgctcc catggcgaga gagatgcgct cccagcgtgc ggtctcggtc tggagctgct
     6961 ttcgtccggc tttggtcagc gcatagaatt tggcgcgccg attggtttcc gcctgacccc
     7021 agctggaggt gagccagccg gcctgctcca tgcgatggag tgcgggaaac agtgagccgg
     7081 ctttcacgtc gaaggcagcg ttcgtcattt gcgcgatgcg gcgggagatg cccatgccgt
     7141 gcagttgctc gagcgcgagg gctttgagga tgaggagatc gagagtgccc tgcaggagat
     7201 cggactgggg accggccatg gggggctcct attgactgtc tataagacaa taggccttct
     7261 cttatagaca gtcaataaga cggcagagag gaattcgatt ctatgttgtg cgcatcaagc
     7321 cggaggagac gtaaagatgg actgcacgct ggtcttcaga cggggcgggg ctgcgtgggc
     7381 catggaatcc ggagctggag gtcatgtacg gcgccgatcg ctatagcaat gcgcaaacca
     7441 ttggtcatta ggggaaatcg cggtgccaag gtatgctcat agcagggcag agcattcatg
     7501 gctcctccct tcccttttgg ccttttctcg atcgctgttt tccggctgag ccggttagcc
     7561 cccagcgaac cctgttgcct cgagctatgg cttgcgtagt tcacgatcca caaccgggaa
     7621 atgaggcaag ggcatgcgat cgaatctggt gtactccgcg ggactgaaga ttgagaaccg
     7681 ctttctgctg gcgactgtgg tgatgcgcgc cgtgcgcaag ctgcacatcg actccacgcg
     7741 gacggaagac acgacgaaca aggtgctggg ccaggtagcg gagggcaagt tcgttgaagt
     7801 ggagttgccg gcgatcgtgc cgcctcctgc ggtggacgtg ctgctgatcg ggtctgcggc
     7861 ttaggtcgaa cctggttctc ggcaaggttg ggctcgaccg ggcgtacgtt cacggatgcg
     7921 gccgatccag actctaagaa acagcgcccc atcttctgtg acaaagaggg agatggggcg
     7981 gtggcgcgtt tggggacggt cgtcgctgtc gatcccgtga ggatcgcgtc acttgatcac
     8041 tgaaatccca aatgtccact gccgcgagcg cgcgtaatta ttcgccgcgt tcactgagtc
     8101 ggagtatttg atcgtcagtt cggaattcag aagattgtcc ttctgaatga gctggaactt
     8161 ctcgaaattc aggttcgaga gcgggatcac gatcgaggca tctccataac cttcgacccg
     8221 ctggctgccc gcctgggtct tatcccgagg cagataccac agccccagat cgatccggga
     8281 gccaacacag aactgtcgcg atgtgctgtc gccggctttg gcaccgatcc acttccgata
     8341 acgcgagcac aaacctggca ggacataaaa aggttcgatg gccagcgccg gattgacgga
     8401 aaagtcatcc ccggacagcc ggggagtggc cgcagtcact tcctgcgcaa agcggtgcgt
     8461 atattgcgcc gcgagggcaa attcaggagc caggggcgcg tcaatccagc tgttgttcag
     8521 cccctggcgc gtccagtacc aggggaccag cccggaaagt gaaagattgc tgacggcgga
     8581 aagtgagctg gccgtttgat ttccctggag ggtctcgctc acctggaccg gcgtgtacca
     8641 ggcggagaga aggccccact gcgagccggc cgtcactgcg aaggagctca tggtgtcctt
     8701 cgcatcggtc gagaagaggt cgtttgtatt gatgaatacc ggcgaatggc caacataggg
     8761 tacgccgaaa gagaccgaga gttggcccac atcgcgggtg gtcccgttcg tgagagcctc
     8821 gccgggcgcg gcctgaggga gaacggagac gctgtaagag gtggaggcgg ggaatgcctg
     8881 agcggcagac acaaggttgg cgccggggga gagtcccgtc agagcggcgt cgggtaagcc
     8941 ttcgagactg acgacatact tcgtattggt atcgactttc tcgcggagca ggagcaggac
     9001 cggtgcggga gtggggcggt cgttgcagtt ttcatgcccc accggcatga ccgccgaaac
     9061 cgggattctc tttgtcgaac ctgaaaccgt caccgcatag gacgaagggc tggcgcagtt
     9121 ccatccatgg tcttcggcac ttcggatgac ggcgacgaat ttctctcgcg tggccaggtc
     9181 gccggtagtt ccattgagga acacctcaat cgatgcggct ggcttcatgc tgggataggc
     9241 agctcccgaa tagaagctga cccgcagagg caggctctcc gaggacgcgg cgttcgccgt
     9301 tggttgggcg gacgatgaag cgggcaggga gaggagggtc cagctcagcg ccacagccat
     9361 ccatgtgcgg tgagcggggc tcatccagtc accacgatc
//
LOCUS       DASZYN010000299         5377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_780043, whole genome shotgun sequence.
ACCESSION   DASZYN010000299 DASZYN010000000
VERSION     DASZYN010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780043"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(451..1755)
                     /locus_tag="VHE33_07775"
     CDS             complement(451..1755)
                     /locus_tag="VHE33_07775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HVT97393.1"
                     /translation="MLRTDSWPVQESSRAESAHTHLAAVLSSLFRDYDGPPFAVRLWD
                     GWCWLSTASKPPACTLCLANAGALHSLLEASQLAVGEAFLHKDIDVEGDLFSAFAPAE
                     HMLSHPAPLRERLLRRLTRGAASLTESLRHGPKYSERRDQDSIAYHYDQPVEFFRPWL
                     GPTLVYSCAYFEQPDDSLDDAQKQKLELICRKLRLQPSERFLDVGCGWGSLILHATAH
                     HHVCADGITLSRSQESVTSQRIEDHSLKNTCRVRLIDYRRLDPAQNTYDKIASVGMYE
                     HVGLSNLPVYFDKMYRLLKPGGVFLNHGIARSPHSTSRGNDSFIAKWVFPDGYLVTLT
                     EAIAAAEDAGFEVRDVENLREHYALTLRHWVRGLTACRNVLLQSVPELTYRIWLLYMA
                     GCAAAFERGDIGVYQTLLSRPDRGRSGLPLTRRDWYSRNLLD"
     gene            1923..2489
                     /locus_tag="VHE33_07780"
     CDS             1923..2489
                     /locus_tag="VHE33_07780"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HVT97394.1"
                     /translation="MHFGDSIFIFLLALVLFGPKKLPEIGRTIGKLLGEFRRASNEFK
                     FQIQEELRNMEEDERRKKRAELEAQKAAEAGTEAGAGVGTEPTATNTTPSILPPSTGV
                     PVSTSAPYATPGAVEEVAPSAAEVVVEPSYPTESERPGLESELDAPAHVDLTAHGNGA
                     HTSITNAEPIESDLPTESGVPSESIHHG"
     gene            2482..3285
                     /gene="tatC"
                     /locus_tag="VHE33_07785"
     CDS             2482..3285
                     /gene="tatC"
                     /locus_tag="VHE33_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HVT97395.1"
                     /translation="MADLVDRARAAVTERAELPGMSLLEHLAELRRRIIHVALYLIGG
                     FFLAWFFHEKIYGFMEKPIVQALAKHHLDTHLVYHNPIDPFNMYLEISFMAGCILACP
                     FVLYEVWLFISPGLYQNEKRYVVPFMTATIGLFLAGAYFGYRWVYPGSLDFLLEFNRD
                     FRAYIEINEYTSLFLTVILGLGITFELPILIMFLSLFGIVSPRFLWKNFRYAVLLIFI
                     IAAIITPTSDILTMCVFASPMLVLYLLGIGVSYLVHPAARKRRAAKADS"
     gene            3282..4172
                     /locus_tag="VHE33_07790"
     CDS             3282..4172
                     /locus_tag="VHE33_07790"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HVT97396.1"
                     /translation="MRVFLLAFLLIASMAGGASAESLPQFDGAKALAYTREYVNATGP
                     RWIGSPGHAKAEAFLKAQFRHDQLEEDTFAVNTPAGTMTMHNFIVKFPGKKDGIIVLA
                     SHYETNYPLRNIHYVGANDGGSTTGLLIEIANHLRAKPNDGYSVWLLFTDGEEAIKSW
                     SDTDSVYGSRHLAVKWQSDGTLKRIKAFILADMLGDRDLNVQRDMNSTPWLEDLIYQA
                     ASQYGWQSYFFENKTYDEDDHLPFVHRGVPCADLIDLNYGYGNSWWHTADDTMDKLSA
                     KSLTIAGDTILETIQLLDRK"
     gene            4176..5066
                     /locus_tag="VHE33_07795"
     CDS             4176..5066
                     /locus_tag="VHE33_07795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HVT97397.1"
                     /translation="MHHPWIPFLIMVAVLLLVDLRLLHGKKGRASAKIAWLWTGILAL
                     IACGFSVYVASTSGKQPALEFLTGYVIEGSLSVDNLFIFLILFRSLHLRAEEQHNVLL
                     WGVMGAIVLRGVLIVAGTSLLNRFAWIQYGFGVLLAVAAVRLLFEKPGELPAHGPLRW
                     IRDRCRPADGGSHLAFSSLLVIILAIEATDLIFALDSIPAVLAVTRDTWIAFTSNIFA
                     VLGLRSLYFALASVLDRFHMLHYGLACILGFVAFKMLAVHWIEVPVGWSLGIIISILA
                     IFVVVSRIVPEPPGHARHTG"
     gene            5127..>5377
                     /locus_tag="VHE33_07800"
     CDS             5127..>5377
                     /locus_tag="VHE33_07800"
                     /inference="COORDINATES: protein motif:HMM:NF019603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HVT97398.1"
                     /translation="MKVGVLGSGTVGQTLAAGFLKHGHEAMIGTREPAKLADWSKANP
                     RGKVGSSSEAAAFGDVLVLAVKGDAAAKVLRDAGVGNLKG"
BASE COUNT         1005 a         1471 c         1772 g         1129 t
ORIGIN      
        1 cttcttccag aacactcaca agtagccgcc ttcagtcctt ttcgggctag cccaattggg
       61 acggggatgt tgaaagccaa acgtgaaacc cggctgcagt cggctcgctg cgattgctcg
      121 cccagccaac ggggatgttg cagcctctgt tgctttagac gcgccgcgtg cgggtaagtc
      181 gcaaaaggac ttgtccgcaa gggcttgtcg ttgccgttag tttaccgcat tggcacctcg
      241 aggttgaaat ccgcaatccc gttcgagata cggtgtgaca caaacaccgt ggctaaagcc
      301 cctgagtttg gagcggctta ttccccgggc tgaagcccgg ggctctttac cgccatcgcg
      361 aggctcaagc ctcacgattc tgggcaccgg aggcatttcg attttgaccg ttgctcgcgc
      421 gtgaacagca tggatgccga cgctgcaggg tcagtcgagg aggttgcggc tgtaccagtc
      481 gcggcgcgtg agggggaggc cgcttcggcc gcgatcgggg cggctgagga gggtttgata
      541 gacgccgatg tcaccgcgtt cgaaggccgc ggcgcatccg gccatgtaaa ggagccagat
      601 gcgatacgtg agctcgggga ctgactgcag taatacgttg cggcaggctg ttagaccgcg
      661 gacccagtgg cgcagggtga gagcgtagtg ctcgcgcagg ttttcgacgt cgcggacctc
      721 gaagccggcg tcttcggcag cggcgatcgc ctcggtgagc gtgaccaggt agccatcggg
      781 aaagacccac ttcgcgatga aggagtcgtt gccgcgtgat gtcgagtggg gagagcgcgc
      841 gatgccgtga ttcaggaaga cgccgcccgg cttgagcagg cggtacattt tgtcaaagta
      901 aaccggtagg ttggacaagc cgacatgctc gtacatgccg acgctggcga ttttgtcgta
      961 agtgttttgg gccggatcga gccggcggta gtcgatgagg cgcacgcggc acgtgttttt
     1021 gagactgtga tcctcaatgc gctggctggt gacagattcc tgactgcggc tgagcgtaat
     1081 gccgtcggcg caaacgtggt gatgcgccgt tgcgtgcagg atgaggctgc cccagccgca
     1141 accgacgtca aggaagcgct cgctgggctg gaggcgaagc ttgcgacaaa tcaattcgag
     1201 cttctgtttc tgtgcgtcat cgagcgaatc gtcgggctgc tcgaaatacg cgcatgagta
     1261 gaccagggtt ggccccagcc agggacggaa aaactcgacg ggctgatcgt agtggtaggc
     1321 aatggaatcc tgatcgcggc gctcggaata ttttgggccg tggcgcagcg attcagtcag
     1381 gcttgccgcc ccgcgggtta agcgcctcag cagccgctcc cgaagcgggg ccggatgaga
     1441 aagcatatgc tcggcggggg cgaaggcgga gaacagatcc ccttcgacat cgatgtcctt
     1501 atgcaggaag gcctcgccca cggcgagctg actggcctca aggagcgagt gcagggcgcc
     1561 ggcattcgcc aggcagaggg tgcaagccgg cggctttgaa gccgtcgaga gccagcacca
     1621 gccatcccac agacgcacgg caaagggtgg gccgtcgtaa tcgcgaaaga gggagctcag
     1681 gaccgccgcc agatgtgtgt gggcagattc agccctcgaa ctctcctgaa ccggccaact
     1741 gtccgtgcgc agcattccgt tcttccaccc atatctttgg attcggggga ggcgctgctc
     1801 gccatggggt acgccgcttg attatgcaac aaccgaccgg gcttgttgat gactttcatg
     1861 ggagatcgcg gaagacggga ttgttgtgac tggcgaatcg cgacaggtat catgaagcat
     1921 tcatgcattt cggcgactcc atctttatct tcctgctggc gctggtgttg tttgggccca
     1981 agaaactgcc tgaaatcggg cggacgatcg ggaagctgct gggggagttc cggcgcgcca
     2041 gcaacgaatt taagttccag attcaggaag agttgcgcaa catggaggag gacgagcggc
     2101 gcaagaaacg cgccgagctg gaggcacaga aggccgccga ggcggggacg gaggcgggag
     2161 ccggggtggg gaccgagccc acagcaacga atacaacgcc gagcattctg ccgccttcga
     2221 caggagttcc ggtcagcacc agcgcgccgt atgcgacgcc gggtgcggtc gaagaggttg
     2281 cgccttcagc tgccgaggtg gtggttgagc cttcatatcc aacggaaagc gaaaggccgg
     2341 ggctggaatc ggaactcgat gcgccggcgc atgtcgacct gaccgcgcac ggcaacggcg
     2401 cgcacacttc gattacgaac gcggagccga tagagtccga ccttcccacc gagtccggcg
     2461 ttcccagcga gtccattcat catggctgac ctggtcgatc gcgcgcgcgc cgcggtgacc
     2521 gagcgcgccg aactgccggg catgagcctg ctcgagcatt tggccgagct gcggcggcgc
     2581 atcattcacg tcgcgctcta cctgattggc ggattcttcc tcgcctggtt cttccacgag
     2641 aagatttacg gcttcatgga gaaaccgatt gtccaggcgc tcgccaagca ccacctggac
     2701 acgcatctcg tctatcacaa cccgatcgac ccctttaaca tgtacctcga gatcagcttc
     2761 atggcgggct gtattctggc ctgccccttc gtgctgtacg aagtgtggct gttcatctcg
     2821 ccggggcttt accagaacga gaagcgctac gttgtgccgt tcatgacggc gacgatcggg
     2881 ctatttctcg ccggggcgta cttcggatac cgctgggtgt atcctggctc gcttgatttt
     2941 ctgctcgagt tcaatcgcga cttccgcgcg tacatcgaga tcaacgaata cacgagcctg
     3001 ttcctgacgg tgattctggg tctgggcatc acgtttgagc tgcccatcct gatcatgttt
     3061 ctttcgctgt tcgggatcgt gagcccgagg tttctctgga agaattttcg ctacgcggtt
     3121 ctgctgatct tcatcatcgc agccatcatt acgccgactt cggacatcct gaccatgtgc
     3181 gtgttcgcga gcccaatgct ggttctgtat ctgctgggga tcggggtttc gtacctcgtg
     3241 cacccggctg cgcgaaaacg gcgggccgcg aaggcggatt cgtgagggtt tttcttctgg
     3301 ctttccttct gattgcgagc atggcaggag gagcttcagc cgagagcctg ccacaatttg
     3361 acggcgcgaa ggcgctggcc tacacgcggg agtatgtgaa tgccaccggt ccgcggtgga
     3421 tcggcagtcc aggccacgcg aaggcggaag cgtttctgaa agcgcagttc cgccacgatc
     3481 agctggagga agataccttc gcggtgaaca cgccggccgg gacgatgacc atgcacaact
     3541 tcatcgtgaa gtttcccggc aagaaggacg ggatcatcgt gctcgcctcg cactatgaga
     3601 cgaattatcc gctgcgcaat atccattacg ttggagcgaa tgacggtggc tcgacgacgg
     3661 gtttgctgat cgagatcgcc aaccacctgc gagcgaagcc gaacgatggc tacagcgtct
     3721 ggctgctgtt taccgatgga gaggaagcga ttaagagctg gtccgatacc gattcggtgt
     3781 atggcagccg gcatctggcg gtgaagtggc aatcggatgg aaccctgaaa cggatcaagg
     3841 cattcatcct ggccgatatg ctgggcgatc gcgatctgaa tgtgcagcgc gacatgaact
     3901 cgacgccgtg gctggaggac ctgatctacc aggcggcatc gcagtatggg tggcagtcgt
     3961 atttctttga gaacaagacg tacgacgagg acgatcatct gccgttcgtt caccgtggcg
     4021 tgccgtgcgc cgacctgatc gatctgaact acggttacgg aaactcgtgg tggcataccg
     4081 ccgacgacac gatggacaag ctgagcgcga agagcctcac catcgccggc gatacgatcc
     4141 ttgagacgat ccagcttctg gacaggaaat aggcgatgca tcatccgtgg attccgttcc
     4201 tgatcatggt ggcggtgctg ctgctggtcg atctgcggct gctccatggg aagaagggac
     4261 gcgcatcggc gaagattgcg tggctgtgga ccgggatcct ggctttgatc gcgtgcgggt
     4321 tctcggtgta tgtcgcgtct acaagcggga aacaaccggc gctggagttt ctgaccgggt
     4381 acgtgatcga aggctcgctg agcgtcgaca atcttttcat ctttctgatt cttttccgca
     4441 gcctgcatct tcgcgccgag gagcagcaca acgtcttgct gtggggggtg atgggtgcga
     4501 ttgtgctgcg cggcgtgctc atcgtggcgg gaacgagcct gctgaaccgg ttcgcgtgga
     4561 tccagtacgg gtttggcgtg ctgctggcgg tggcagccgt tcggctcctg ttcgagaagc
     4621 ccggcgaact gccggcgcac ggcccgctgc ggtggattcg ggaccgctgc cggccggcgg
     4681 atggcggttc acatctggcg ttctcttccc tgctggtgat catccttgct atcgaagcga
     4741 cggacctgat tttcgcgctc gattcgattc ctgcggtgct tgcggtgacg cgtgacacct
     4801 ggattgcgtt cacgtcgaat attttcgcgg tgctgggatt gcggtctctg tattttgcgc
     4861 tggcgtctgt gctggatcgg ttccatatgc tgcattacgg actggcgtgc attctcggat
     4921 tcgtcgcctt caaaatgctg gcagtgcact ggatcgaagt accggtcggg tggtccctgg
     4981 ggatcatcat ttcgattctc gcgatctttg tggtggtcag ccgcattgtt cctgagccgc
     5041 ccggccatgc tcgccacacg ggatgacggt ttcgattttc atcgtgaccg cgctatagtt
     5101 tttgcacttg tgctggagga aaagggatga aggttggagt gttgggttcg ggaacggtgg
     5161 ggcaaacact ggccgcgggt tttctgaagc acggtcacga ggcgatgatc gggacgcgcg
     5221 agccggcgaa gctggccgac tggagcaagg ccaatcccag ggggaaggtt ggcagcagca
     5281 gcgaagcggc tgcgttcggt gacgtcctgg tgctggccgt gaagggcgac gccgccgcga
     5341 aggtgctgcg cgacgccggc gttggaaatc tgaaggg
//
LOCUS       DASZYN010000300         6733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1106023, whole genome shotgun sequence.
ACCESSION   DASZYN010000300 DASZYN010000000
VERSION     DASZYN010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106023"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VHE33_07805"
     CDS             complement(<1..770)
                     /locus_tag="VHE33_07805"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HVT97399.1"
                     /translation="MKKLGSDWRFLLIFGGVVAALIVVTGILAPNREDRDPTPSTWNS
                     GSAGAKAAFLLLSQLGYNTARWERPEAELSSIDASHATLILAEPSRSLTALTDKDRQQ
                     PFVDFLHRGGRIVATGLVAALFLPDAKVTQAERLFTDLCITTPEGPDSLARAGELEMA
                     APVRWNGDEPSVRVSQTCGGGAVVVSYREGNGEIVWWASATPLTNMGLHNDGNLRLLL
                     ASVGDPGRTIYFDEFLHGVNTSPWTATHGTPLIGIAIQT"
     gene            complement(767..1705)
                     /locus_tag="VHE33_07810"
     CDS             complement(767..1705)
                     /locus_tag="VHE33_07810"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HVT97400.1"
                     /translation="MPAISLADDAHVTVGERTLKPSTLADYSGDLHSMRSLAAACAQN
                     ASACDPARVSADEHVTLANKSSFDAHFAWMTDALSSAKSLPNSRRAALMADAEAHLDE
                     DMADAQAVPAAPNFSKARKEADTILASREFNTSTAPSLRDQIIALLYRWLDRLLAHVA
                     AFGSRSPWIGPLLEWGLGAIACSLLLVWAFRTMRRQRVRMQRDATRRTEQTDERVLNW
                     MREAEEHAAGGRFRDAVHCLYWASIVTLEGRRFWQPDRARTPREYLRLLDPASAAAPL
                     LRRQTFTFETIWYGLRPAARPDYDNALELHRQLRAA"
     gene            complement(1753..2763)
                     /locus_tag="VHE33_07815"
     CDS             complement(1753..2763)
                     /locus_tag="VHE33_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97401.1"
                     /translation="MAGGQSWAPSESLAQTTSGYDLRPLSTGEVLDRTFQLYRSRFAL
                     FASLAALPAGVSFLSGALQVLFTMGERRLRNGIQIQTVTRPVLYGVIVTIGVLLYFAC
                     YGITQAATTWAVAEIYLGKPASVGAAWKAATTRWFRYVLVTLRQYWSLAWWFLLSLSM
                     FFAVLALVPRIGGGSAIAVGILSFVFGLQIFASAIYAVYAAIRVSLAVPASVIESLGP
                     NAAVRRSMTLLTQRKGRIFLLGLLLLAMSMVVGVLLTPLAFIGLRAHGPERYILQMIQ
                     LGGAFLSRLLVGPVGAIALCLFYFDERVRHEGFDIEFLMLRAGPPPGDPPAEPNPSPE
                     PA"
     gene            complement(2878..4443)
                     /locus_tag="VHE33_07820"
     CDS             complement(2878..4443)
                     /locus_tag="VHE33_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97402.1"
                     /translation="MTQRHLRFLPVFLLSTLGFAAGMRSLAAQTSSESLGLFDGQSDV
                     GSVTPSGSAVYDAAASSYTLTAAGANLWGTADAFHFVWKKMSGDTVLTADIDFPETTG
                     EHNPHRKAILIIRQNLDANAAYADAAVHGVGLTALQFRREAGAMTEDLELSNTTAPRR
                     LRLEKRGDTLTMYVASEPGGALHQVGASVQLHLAEPFYVGLGVCSHDPSRTEKAVFSH
                     VTIESPEAPASAPVLYSTLNTIGDDKDFRRVEIVWSAQGHAEAPNWSRDGKTLIFDRE
                     GKIMTIPVEGGTPQVLDTGEASRCTGSHGLSPDGTHLAMTCATTDSKEFRIYIVPITG
                     GAPRILTEHPNSWFHSWSPDGKTILFTRPDHGSLNVFAIPAAGGEEHALTTGTGVSDD
                     PDYAPDGQFIYFNSDRDGSMQIWRMHPDGSSPEQMTHDEWVNWTPHPSPDGKSIVFIS
                     YEHGTAGHPTNQPVALRVMSLADRKVHILFRLTGGGGTMNVPNWSPDSKRLAFVSYQM
                     LPAAAGEQGGTHP"
     gene            4656..5447
                     /locus_tag="VHE33_07825"
     CDS             4656..5447
                     /locus_tag="VHE33_07825"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HVT97403.1"
                     /translation="MLQLKRAEWAGLVSVVVLAALLLVRAPHDPLAYWTIAGSAIMVY
                     AGLQESDRRDLLWLAVLLPASIFAIDGGNITVTSMSHHTLDAVLSRMDSGRSVWFYHW
                     TLAHGWCQLILVNGYESLPFFVGFVMSTSTKRSRCAIALVIASVVAPLFYLAFPAVGP
                     AHVGSLTAPRNCMPSLHMTWALICLMLIEPRLRIVGVLFAVLTAFATIGTGEHYIVDL
                     AAAVPYTVAVCWIAGRCERWRASVSRSRLQEAAGIWKNGSGRVGV"
     gene            complement(5467..5543)
                     /locus_tag="VHE33_07830"
     tRNA            complement(5467..5543)
                     /locus_tag="VHE33_07830"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5507..5509),aa:Arg,seq:ccg)
     gene            complement(5550..6497)
                     /gene="mmuM"
                     /locus_tag="VHE33_07835"
     CDS             complement(5550..6497)
                     /gene="mmuM"
                     /locus_tag="VHE33_07835"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF007020.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HVT97404.1"
                     /translation="MTPRDQLDFRSIRVLDGAMATELEQRGCDISGSLWSAHVLEDAP
                     AAIVSVHLDYLRAGADCISTASYQVSSLGYRELGRPAADSVHALQQSVALAGQARTVF
                     ARENPRRVWIAASLGPYGAALHNGAEYHGRYAIGFDDLVAFHAERFDVLAGTGADLLA
                     LETIPSLEEAQALLHALRLFPALSAWISFTCRDAALVAHGEPLHECAETLAESEQVAA
                     IGINCTAPRHVEALIGEARRGCGDRKSVIVYPNSGETWDATKRTWNGASDPREYGALA
                     RAWYAAGAQAVGGCCRTRPAHIVAVAQAVGKHGLLPVVS"
BASE COUNT         1241 a         1963 c         2333 g         1196 t
ORIGIN      
        1 gtttggatgg cgatgccgat gagcggagtg ccgtgcgtcg cggtccatgg actggtattg
       61 acgccgtgga ggaactcgtc gaaatagatc gtgcgtcccg gatcgccgac gctggccagc
      121 agcaaacgca ggttgccgtc gttatgcagg cccatgttcg tgagcggcgt ggcggaggcc
      181 caccagacga tctccccgtt gccttcgcga taggagacga cgaccgcgcc gccaccgcag
      241 gtttgcgaaa cgcggaccga tggctcgtcg ccattccagc gcacgggtgc agccatctcc
      301 agttcaccgg cgcgcgccag cgagtccggg ccttccgggg tggtgatgca caggtcggtg
      361 aagagccgct cggcctgggt cactttcgcg tcgggaagaa aaagcgcggc gacgagtccg
      421 gtggcgacga tgcgtccgcc acgatggaga aaatcgacga atggctgctg gcggtctttg
      481 tcggtgagcg cggtgagcga tcgcgaaggc tcagcgagga tgagcgtcgc atgggatgca
      541 tcgatggagg agagttccgc ttccgggcgc tcccaacgag ccgtgttgta gccgagctgg
      601 ctcagcagca gaaacgccgc cttcgctccc gcgctgccgg agttccaggt gctgggtgtc
      661 gggtcgcggt cttcgcgatt gggtgcaaga attcccgtta cgacaatcaa tgcagcgacc
      721 acgccgccga agatgagcag gaatcgccag tcgctgccga gcttcttcat gcggccctca
      781 actgccgatg cagttcgagt gcgttgtcgt agtccgggcg cgccgccgga cgcaggccgt
      841 accagatcgt ctcaaaggtg aaggtctggc gccgcagcag tggcgctgcg gcagaggcag
      901 gatcgaggag gcgcagatac tcccgcggtg tgcgcgcacg gtccggctgc cagaaacggc
      961 ggccttcgag ggtaacgatg ctcgcccagt agaggcagtg caccgcatcg cggaagcggc
     1021 caccggcggc gtgttcttcg gcttcgcgca tccagttcag gacgcgctcg tcggtttgtt
     1081 cggtgcggcg ggttgcatcc cgctgcatgc ggacgcgctg ccggcgcatg gtgcggaaag
     1141 cccacaccaa cagcaaggag caggcaatcg cgcccaggcc ccactcgagg agaggcccga
     1201 tccacgggga acgcgagcca aacgcggcca catgcgcgag aagccggtcg agccagcgat
     1261 acagcaaggc aatgatctgg tcgcgcaaag aaggcgcggt ggaggtgttg aactcgcggc
     1321 tggccaggat ggtgtcggcc tcttttcggg ctttcgagaa gttgggcgcc gcagggacgg
     1381 cctgggcatc ggccatgtct tcgtcgagat gcgcttcggc gtcggccatc agggcggctc
     1441 gccttgagtt cggaagcgat ttcgcggacg acaatgcatc ggtcatccat gcaaagtgcg
     1501 catcgaacga ggatttgttc gccagggtga cgtgctcgtc ggcactcacg cgggccggat
     1561 cgcaggcgga tgcattttgc gcgcaggccg cggccagcga tcgcatggaa tgaagatctc
     1621 cggaatagtc ggcgagggtg ctcggcttca acgtgcgctc tccgaccgtt acgtgcgcgt
     1681 cgtcagcgag tgagatcgcg ggcatcgcca gcagaagccc aacaacggcg acagtggtgc
     1741 ggaaacggtg gatcaggcag gctccggaga tggatttggc tccgcgggag gatcgccggg
     1801 cggcggaccg gcgcgcagca tcagaaactc gatgtcgaag ccctcgtggc gcacgcgctc
     1861 atcgaaatag aagaggcaca gcgcgatggc gccgacgggt ccaacgagga ggcggctgag
     1921 gaatgcaccg ccgagctgga tcatctgcag gatgtagcgc tcgggtccat gcgcgcgcag
     1981 gccgatgaaa gccagaggcg tgagcaggac gccgacgacc atactcatag caaggagcaa
     2041 caggcccagg agaaagatgc ggcccttgcg ctgggtgagc agagtcatgc tgcgccgcac
     2101 cgcggcgttg ggaccgagcg attcgatcac tgaggcgggc acggcgagtg agacgcggat
     2161 ggcggcgtag acggcgtaga ttgcactggc gaagatctgg agtccaaaga cgaaggagag
     2221 gattccgacc gcgatggccg agccgccgcc gatgcgcggc accagcgcga gcaccgcgaa
     2281 gaacatgctc agcgacagca ggaaccacca ggcgaggctc cagtactggc gcagggtcac
     2341 gagcacatag cggaaccagc gtgtggtggc agctttccat gcggcgccca ccgatgccgg
     2401 cttgccgagg taaatctccg caaccgccca ggtggttgca gcctgggtga tcccgtagca
     2461 ggcgaagtag agcagcacgc cgatggtgac gatgacgccg tagagcacgg gtcttgtgac
     2521 ggtctgtatc tgaatgccgt tgcgcaggcg gcgctcgccc atggtgaaca gaacctgcag
     2581 agcgccgctg aggaaactca cgcctgcggg aagcgcagcc aggctggcga aaagcgcaaa
     2641 tcgcgaccgg tagagctgaa aggtgcggtc gagaacttca ccggtggaaa gaggccgaag
     2701 atcgtaaccg gaagtggtct gcgcgaggct ctccgaaggt gcccagcttt gcccgcctgc
     2761 catgagaggt gactccgagt gtgggcatgg tagcacagca gcggcgcgaa ggggacagcc
     2821 cgttcgaggg atcatggcat tcgttccgca cgcggtcggc ggaacgttca cgccttgtta
     2881 tggatgtgtg ccgccctgct cgccagccgc tgcgggcagc atttgataac tcacaaaggc
     2941 aaggcgtttg ctgtccggcg accagttggg aacgttcatg gtgcccccgc cgccggtgag
     3001 gcggaagagg atgtgcacct tgcgatccgc gagcgacatt acgcgcaggg cgacgggttg
     3061 attcgtggga tggccggcgg ttccgtgttc atagctgatg aagacgatcg atttcccgtc
     3121 cggggaagga tgcggcgtcc agttgaccca ttcatcgtga gtcatctgtt cgggggagct
     3181 gccatccgga tgcatgcgcc agatctgcat cgagccatcg cgatcggagt tgaagtagat
     3241 gaactgccca tcgggcgcgt agtcgggatc gtcgctgacg ccggttcccg tggtgagggc
     3301 gtgctcttcg ccgcccgccg cgggaatcgc aaagacattc agcgagccgt gatcgggccg
     3361 ggtaaagaga atcgttttgc cgtcgggcga ccagctgtgg aaccaggaat tggggtgttc
     3421 ggtcaggatg cggggcgcgc cgccggtgat ggggacgatg tagatgcgga actccttgct
     3481 gtcggtagtc gcgcaggtca tagcaaggtg cgttccatca ggcgaaaggc catggctgcc
     3541 ggtgcagcgg ctggcctcgc cggtgtcgag cacctgaggc gtaccgcctt cgacggggat
     3601 ggtcatgatc tttccttccc gatcgaagat cagggttttt ccgtcgcgac tccagttcgg
     3661 cgcctcggcg tgcccttgcg cggaccaaac aatttcgacc cggcggaagt ctttgtcgtc
     3721 gccgatggta ttcagtgtgc tgtacagaac cggcgcggac gccggcgcct cgggagactc
     3781 gatcgtcaca tgagagaaga ccgccttctc cgtccggctg ggatcgtggg agcagacgcc
     3841 gaggccgaca tagaacggtt cggcaagatg gagctgcacg ctggcgccca cctggtgcag
     3901 cgctcctccg ggttctgaag ctacatacat ggtcaatgtg tcgccgcgct tttccagacg
     3961 cagacggcga ggcgccgtcg tgttgctcag ctccaggtct tcggtcatgg cgcccgcttc
     4021 acgccggaac tgcagcgccg tgaggccgac accatggacg gctgcatccg cataggctgc
     4081 atttgcgtcg agattctggc gaatgatcag gatggccttg cggtgaggat tgtgctcgcc
     4141 ggtagtctca ggaaaatcga tgtctgcggt gaggaccgtg tcgccggaca ttttcttcca
     4201 gacgaaatga aacgcatcgg ctgttcccca cagatttgcg ccggcggctg tgagcgtgta
     4261 actgctggcg gcggcatcat agaccgctga tcccgatggt gtaacggagc ccacgtccga
     4321 ttgaccgtcg aagaggccaa gagactccga ggacgtttga gccgcaaggg accgcatgcc
     4381 ggcggcgaat ccaagtgtgc tgagaagaaa taccggcaga aagcgaagat gtcgctgcgt
     4441 cacgcatgcc tccgcgcaat gtgcgtaccc atgatgtcac acgtaccccc aaacggggga
     4501 tcgtgagacg cattgccgcc cggtagcatg aggccagtga tggctactgg ttcgattggg
     4561 gccgggctgc gccgctcgca tgccaaagcg gctgatgacc ggcgacggca ccggaacagg
     4621 gaatcgaacc gcaggtttcc gcagatgtgg agagggtgtt gcagttgaag cgcgccgagt
     4681 gggcaggtct ggtttctgtg gtcgtcctgg ctgcattgct gctggtgcgc gcaccgcacg
     4741 atccgctggc gtactggacc atcgccggca gcgcaatcat ggtgtatgcc gggttgcagg
     4801 agtccgaccg gcgcgatctg ctgtggctgg cggttctgct gcctgcctcg atctttgcca
     4861 tcgacggcgg caacatcacg gtgacttcga tgagtcacca cacgctggat gccgtattaa
     4921 gcaggatgga ttccgggcga agcgtctggt tctaccactg gaccctggcg cacggatggt
     4981 gccagctgat tctcgtcaac ggctacgaga gcctgccctt ctttgtcgga tttgtgatga
     5041 gcacgagcac gaagcgaagc cgctgtgcga tcgccctggt gatcgcttcc gttgtggcgc
     5101 cgcttttcta cctggctttt cctgccgtgg gacctgcgca tgtcggcagt cttacggctc
     5161 cgcggaattg catgccgtcg ctgcacatga cctgggcgct gatctgcctg atgttgattg
     5221 agccgcgcct gcgaattgtg ggagttctgt tcgcggttct gaccgccttc gccacgatcg
     5281 ggacgggtga gcattacatc gttgatctgg cagcggctgt gccttacacg gtcgcagtgt
     5341 gctggatcgc tgggagatgc gagaggtggc gtgcatcggt gagccgctct cggctgcaag
     5401 aggcagccgg gatctggaaa aatggctctg gaagagtggg cgtttgaggg cggaaagctt
     5461 ttcggatggt gcgcccggag agattcgaac tcccgacctg atgctccgga ggcatccgct
     5521 ctatccagct gagctacggg cgcgttcctt caggatacta cgggcagcag gccgtgtttc
     5581 ccaacggctt gggccacggc cacaatgtgc gcgggccgtg tgcggcagca gccgccgaca
     5641 gcttgcgccc cggccgcata ccacgcccgt gcgagagcgc catactcacg tggatcggaa
     5701 gccccgttcc aggtccgctt cgttgcgtcc cacgtctcgc cggagttggg atagacgatg
     5761 accgatttcc ggtcgccgca tccgcgtcgc gcctccccaa tcagagcctc cacgtgacgg
     5821 ggcgctgtgc agttgatccc gatggccgcg acctgctccg actccgccag ggtctcggcg
     5881 cattcgtgca gtggctcgcc atgtgcgacg agcgcggcat cgcgacaggt gaacgaaatc
     5941 cacgccgaca gggcgggaaa caaccggagc gcgtgcagca gtgcctgtgc ctcctcgagc
     6001 gacgggatcg tttccagcgc aagcagatcg gcgccggtgc ccgccagtac gtcgaatcgc
     6061 tcggcatgaa acgccaccag atcgtcaaag ccgatcgcgt agcggccatg gtattcggcc
     6121 ccattgtgca gcgccgcgcc atagggcccc agagacgctg cgatccacac gcgccgcgga
     6181 ttttcccgcg cgaagacggt tcgcgcctgc ccggcgaggg cgaccgactg ctgcagcgcg
     6241 tggaccgaat cggcagcggg gcggcccaac tcgcggtagc cgagcgaaga cacctgatag
     6301 ctcgccgtcg agatgcagtc ggcgcccgcg cgcaggtaat cgagatgcac agaaacgatt
     6361 gccgcaggag catcctcgag gacgtgcgcc gaccacagcg agccggagat atcgcatccg
     6421 cgctgctcga gttcggtggc catggcgccg tcaaggacgc ggatgctgcg gaagtcgagc
     6481 tggtcgcgtg gagtcaaagt cgttggcggc cctgagcatc gagccggggc gcagactggc
     6541 ccgaggcttt cattttctat tgtggtgcag gagggaggtg gggggaaggc tgccgatcag
     6601 aggctgttgc caacctgatt gaagaagttt ccgatggatc tggtcaggct gttcatggaa
     6661 gcaatcagtg cgagcgccat gacggcggcg atcagagcat attcgatcag gtcctggccg
     6721 gactcatccc gga
//
LOCUS       DASZYN010000301         2051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_203403, whole genome shotgun sequence.
ACCESSION   DASZYN010000301 DASZYN010000000
VERSION     DASZYN010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203403"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..525
                     /locus_tag="VHE33_07840"
     CDS             169..525
                     /locus_tag="VHE33_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HVT97405.1"
                     /translation="MKMILAASAAAFVAIGSAPLNFVATQAPQRIEVTAKRFSYEPET
                     VTVKKGEPVELVLKSEDVPHGLKFRELGVDVKAKKGKTGEVEFTPEKTGDFVGHCSVF
                     CGMGHGSMHLTLHVVE"
     gene            528..1001
                     /locus_tag="VHE33_07845"
     CDS             528..1001
                     /locus_tag="VHE33_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVT97406.1"
                     /translation="MRRGREVGAAAVAVAVMLAAGCKATPPNAFETRLMTVAKHRVLV
                     GDKKLKNPLAANTETIAAGKEAFGHYCVACHGMDGQNTGVPFVTSPPAPSLASREVQS
                     YTDGQLKWVIENGIWPSGMPGSNGTLSDEEQWSIVLFLRHLPPAGSVGEPEMYTH"
     gene            1008..>2051
                     /locus_tag="VHE33_07850"
     CDS             1008..>2051
                     /locus_tag="VHE33_07850"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HVT97407.1"
                     /translation="MVRIRRQAGVVVLAALVAGNVVLRAQSTAGAGSTVTQSSENGAG
                     INAGENARTREDALGRDQGPGSGATASAGLALTARQIFAVLDEKPEVVIELKTQLAEQ
                     LQQQGVAIDPNAVTDEMLYNAIATDPMVREKTTLFLRARGYVSGAGLTQTGDGIPVVA
                     GMGQFSDFGEVPDTPPALLGSNPGVANLQSATSPGGWSGSAAAVDTNRSPASGTKTKA
                     AVGGTRERNITDPPEVLREPTPYNLQSLRDLYTQIPDSSQTLRRFGSDVFVRRSAGTA
                     SRLGGSTSVPSLDVPIGPDYVLGPGDSLRIDLWGGLSQSFTRVVDRSGRLMLPEAGEV
                     QVAGLTLERAQNTI"
BASE COUNT          409 a          496 c          786 g          360 t
ORIGIN      
        1 cccaacagaa tcctgacaat cgcctgacag acacatgaca cggcattgcg aggccgtgga
       61 tagcgtggga acagcgtggc attgcgatgc aattgtcacg aacctgactt ttgtcgtgtc
      121 gcggctggtt gcaggcctcg aaaggctgaa gcttgctgag gagatgagat gaagatgatt
      181 ctggccgcca gcgcggctgc atttgttgcg attggatctg cgccgctgaa ttttgttgcg
      241 acgcaggcgc cgcagcggat tgaggtaacg gcgaagcggt tctcgtatga accggaaacc
      301 gtgacggtga agaagggcga gccggtggag ttggtgctga agagcgagga cgttccgcac
      361 gggctgaagt tccgtgaatt gggcgtggac gtgaaggcga agaaaggcaa gacgggcgag
      421 gtggagttca ctccggaaaa gaccggcgat ttcgttgggc actgttcggt gttctgcggg
      481 atgggacacg gatcgatgca cctgacgctg cacgtggtgg agtagggatg cggcgcgggc
      541 gcgaagtggg agcggcagcg gtggcggtcg cggtgatgct ggcggcgggg tgcaaggcga
      601 ctccaccgaa tgcattcgag acgcgcctga tgacggtggc gaagcatcgg gtgttggtcg
      661 gagacaaaaa gctgaagaat ccgctggcgg cgaatacaga gacgatcgcc gctggaaagg
      721 aggcgtttgg gcactattgt gtcgcgtgcc acggcatgga tgggcagaac acaggggtgc
      781 cgtttgtgac gtcgccaccg gcgccatcgc tggcttcgcg ggaggtgcag agttacacgg
      841 atgggcaatt gaagtgggtg atcgaaaacg ggatttggcc ttcgggaatg ccgggatcga
      901 atgggacgct gagcgacgag gagcagtggt cgattgtgct gttcctgcgg catctgccgc
      961 cggcgggcag cgtgggcgaa ccagagatgt acacacactg aggcgcgatg gtgaggatac
     1021 gaagacaggc cggtgtggtg gtgcttgcgg cgctcgtcgc aggaaacgtc gtgctgcggg
     1081 cgcagagcac cgcgggcgca gggagcacag tcacgcagtc ttcggagaac ggcgccggga
     1141 ttaatgcagg cgaaaacgcc agaactcgag aagatgcgct gggccgtgat caggggccgg
     1201 gctcgggagc aacggcaagc gctgggttgg cgctgacggc gcggcagatt ttcgccgttc
     1261 tcgatgagaa gccggaagtg gtgatcgagc taaagacgca gctggcggag caactgcagc
     1321 agcagggggt agcgatcgat ccgaacgcag tcacggacga gatgctgtac aacgcgatcg
     1381 cgacggatcc gatggttcgg gagaagacga cgctgttctt gcgggcgcgt ggctatgtgt
     1441 cgggtgcagg gttaacgcag acgggagatg gaatcccagt tgtggctggg atggggcagt
     1501 tctctgactt tggcgaagtg ccagatacgc cgccggcact gctgggttcg aatccgggag
     1561 tggcgaacct gcaatccgcg acgagccccg gcggatggtc agggtcggca gccgcggtgg
     1621 acacgaatcg ttcgccggcg agcgggacaa agacgaaggc agcggtgggt ggaactcggg
     1681 agcggaacat caccgatccg ccggaggttt tgcgagagcc gaccccatac aacttacagt
     1741 cgctgcgcga tctgtacaca cagattcccg attcgtcaca gacactgcga cgatttgggt
     1801 cggatgtgtt tgtgcggcgc agtgcgggca ccgcgtcgcg gctgggtggg tccacgagtg
     1861 tgccgtcgct cgacgtgccg attggaccgg attatgtact ggggccagga gacagcctgc
     1921 gcatcgattt gtggggcgga ttgtcgcaga gcttcacgcg cgtggtggat cggagcgggc
     1981 ggctgatgct gcccgaggct ggcgaggtcc aggtggcggg gctgacgctg gagcgcgcgc
     2041 agaacacgat t
//
LOCUS       DASZYN010000302         1510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_912785, whole genome shotgun sequence.
ACCESSION   DASZYN010000302 DASZYN010000000
VERSION     DASZYN010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912785"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..>1510)
                     /locus_tag="VHE33_07855"
     CDS             complement(92..>1510)
                     /locus_tag="VHE33_07855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVT97408.1"
                     /translation="LHNSSLLQAERKIEVLQTLVSVGQEIGSTLNQQRVLQAIVNQPQ
                     LVIPYERAALALEQRNRLTIQAVSGVTKLDASEPSMERLGEILRWVGGIDTEIQVSQH
                     EEEIDDARPETREKFRRYFSESGYRGFYAIPLADDEGRLGVLCFESSDPDFLNDLHIE
                     VIRILASQATLALRNASLYKEVPFIGILEPLIEKKRRFMAIDRRRRALILALAAALVL
                     ILIVVPVPMRVSGDAQVAPDQTEYVRAEEDGVVQQVFVHEGERVAPGTPLAQMADWPA
                     RAALAAIQARYNTAMAQAADALVRNDTTAAGQHQLDANYLRGELARAAEQVSRMTLRA
                     QVAGVVATPHVENLAGRKLALGDPIMEIVSTQNVVADVAVPERDISLLRVGDSARVKL
                     EAFPTQTFSGTVNVVSPAGQVVGENRAFYARVAVPNPDGHLRPGMQGFGKVRTGLRPL
                     GYVIFRDPALWVWSKLWGWFGW"
BASE COUNT          301 a          518 c          409 g          282 t
ORIGIN      
        1 gttcgcggac tgtgcccaga cgagcagccc acactgaaga ggattccgat ggcacccgcc
       61 atggctgcgc tacgacgcct catataagtc ttcaccatcc aaaccatccc cagagtttcg
      121 accacaccca gagtgccggg tcacggaaga tcacataacc gagcggccgc agaccggtgc
      181 gaactttgcc gaagccctgc atccccggac gcagatgccc atcgggattc ggcaccgcaa
      241 cgcgggcata aaatgcgcga ttctcgccga ccacctgtcc ggccgggcta accacattca
      301 cggttcctga gaatgtctgc gtcggaaacg cctcgagctt gacgcgcgcc gaatccccca
      361 cccgcagaag actgatgtcc cgctccggca ccgccacatc ggcaacaacg ttttgcgtgc
      421 tgacgatttc catgatcgga tctcccagcg ccagctttct gccggcgagg ttctcgacat
      481 gcggtgttgc caccactccc gccacctgtg cgcgcagcgt catccgcgaa acctgttctg
      541 cagctcgggc cagctcaccg cggaggtaat tcgcatcgag ttgatgttgt ccggccgcgg
      601 ttgtgtcgtt ccgcaccagc gcatcggccg cctgcgccat ggcagtgttg taccgcgcct
      661 gaatggcggc cagtgctgcc cgtgcgggcc agtcggccat ctgcgccaaa ggcgtgcccg
      721 gcgcaacgcg ctcgccctca tgcacgaaga cctgctgcac caccccatcc tcttcggcac
      781 gcacgtattc cgtttgatcg ggagccactt gggcgtcgcc cgaaacccgc atcggcaccg
      841 gaacgacgat cagaatcaga accagcgcgg ctgccagcgc gaggatgagc gcccgccgcc
      901 gcctgtcgat cgccatgaag cggcgctttt tctcgatcag cggctccagg attccaataa
      961 acgggacttc cttataaaga gaggcattgc gcagagccag cgtggcctgg ctggcgagaa
     1021 tgcgaatgac ctcaatatgc agatcgttca gaaaatcggg atcggagctt tcaaaacaca
     1081 aaacccccag ccgtccttca tcgtcggcca gaggaatcgc gtagaaaccg cgatagccac
     1141 tctcagaaaa atagcggcga aacttctcac gcgtctcagg ccgcgcatcg tcgatttcct
     1201 cttcgtgctg tgacacttga atctcggtat cgatgccgcc cacccagcgc aggatctctc
     1261 ccagccgttc catcgacggt tccgaagcat cgagcttcgt gactcccgac accgcctgaa
     1321 tggtgagccg gttccgctgc tccagcgcga gcgcagctcg ctcatacgga atcaccagct
     1381 gcggctgatt cacgatcgcc tgcaggacac gctgctggtt cagtgtcgaa ccgatctctt
     1441 gccccacgct caccagcgtc tgcagcactt cgatttttcg ttctgcctgc agcagcgacg
     1501 agttgtgcag
//
LOCUS       DASZYN010000303        11929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_76235, whole genome shotgun sequence.
ACCESSION   DASZYN010000303 DASZYN010000000
VERSION     DASZYN010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 11929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11929
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76235"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VHE33_07860"
     CDS             <1..496
                     /locus_tag="VHE33_07860"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HVT97409.1"
                     /translation="TRVPTPGTFSHQAESVHPGGANLMSMTAGSNRTLNADPNVTPLI
                     DVLLVLLIIFMVIVPVTPRGLRAVLPQQPSQAHPHPTRTIVVEILASPSGPAYRINQQ
                     NIADKAALGAELNRIYSIRAEKVLFIQGDPKLDFAAIAEVLDLGRALAIDHIGILTPH
                     LRAG"
     gene            complement(542..1093)
                     /locus_tag="VHE33_07865"
     CDS             complement(542..1093)
                     /locus_tag="VHE33_07865"
                     /inference="COORDINATES: protein motif:HMM:NF025227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HVT97410.1"
                     /translation="MKKTVLTFGLIAGVMISVLMGGSLLIANRIGSGHSMVLGYTIMV
                     ASFLLVYFGIRSYRDNTLGGQISFGRAFACGILITLITTVCYVGMWEILYFNFMPHFM
                     DSYFAAQIHRVEASGLDAATIAAQVAAIQRSQQLYQNPLVNMAYTFMEPLPVGLIITL
                     ISAAILRRKAPGESAATRGVMTT"
     gene            complement(1216..1686)
                     /locus_tag="VHE33_07870"
     CDS             complement(1216..1686)
                     /locus_tag="VHE33_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HVT97411.1"
                     /translation="MKRHVLIFGVVGGLLIAALQYTEYRFVVIEHSVELYGALIAILF
                     ASFGIWLGLRITRSREIIRETVVVREVLVPAEAPAAGPFTPDAARQQSLGITARELEI
                     LTLVARGLSNREIGAQLFVSENTVKTHCARAFDKLGVVRRTQAVQRGKELGLLP"
     gene            complement(1757..3052)
                     /locus_tag="VHE33_07875"
     CDS             complement(1757..3052)
                     /locus_tag="VHE33_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HVT97412.1"
                     /translation="MSVAGIDFGTTNSSVALVMGESRVELASFTFAGAETLSSRSVLY
                     FEQVASAGGMKRTHSWSGPAAIERYLDADDKGRLVQSLKSHLSSRTLTGTEVFGRRHR
                     LEELISRMLSDLRKHAEKQFGRPIRRAVVGRPVRFVGAETTDDDDFAVARLREAFQIA
                     GFEAVEFELEPVGAAYAYESTLDHDELILIGDFGGGTSDFSLLRVGPGARVRGQRQVL
                     GYSGVGLAGDAFDARIVRKLVSPALGSHSEARSLDKILPAVPAWIYANLERWHYLSFL
                     RTNNVREILRGARIRGLEPEKIEALIAVIEQDLGYQLHQAVQRLKFALSQQESAEFRF
                     QEGGLDLRVPLTRREFETWIAEDLGKIEDAVDSLIRQTGVSPGEVDRVFLTGGTSLVP
                     AVRRIFTRRFGREKIRGGHEFTSVAYGLALCAQERGAGQ"
     gene            complement(3240..4694)
                     /locus_tag="VHE33_07880"
     CDS             complement(3240..4694)
                     /locus_tag="VHE33_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HVT97413.1"
                     /translation="MPKAVSSFELTLKSRSRHQTLTHWLYGELRSAILDGRLAAGTRL
                     PSSRDFARQYELSRGTVVSVLERLQAEGYVSSRVGFGTWVNRVEPPRPARQATTTPAY
                     IRRVLSTYKRPQSWVDLAFADKIRPFRIGVPAISEFPSEIWGRIAADRARHFRSWLKK
                     EADRSGYRPLRAAIAEYLRTARGVRCTAEQVVIVSGIQQALDLLARLLLKRGDRIWME
                     DPGYFGARIAFTSAEARMVAVPVDDEGLSVAAGIKACPDAMGAYVTPAHQFPLGVTMS
                     LERRMALLSWAARSGAFVIEDDYDSEYRFEGPPVPALQSLDNHSSVIFVGSFSKTLFP
                     ALRVGYVVLPAPLIDGFLGFRYRTDFRNSSFDQAVLCDFIVEGHLARHLRRMRNLYAE
                     RLAALMEGAMQHLGGLLEISNVRAGLYTIGYLRNEMTSQEAEKLAAAQGIEVLAVDRC
                     TLRRPDPHALLLGFGGFNEPDIRHGLIRLAKALS"
     gene            4835..5656
                     /locus_tag="VHE33_07885"
     CDS             4835..5656
                     /locus_tag="VHE33_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97414.1"
                     /translation="MKVGVWFYGLGLALTGILDIVWRQFDASHQPIQSLGKVPDQNIL
                     ACIVGVWLVAAGLAILWRRSARFGAAASAGACLLFVIFWLARYYAGIHALGWRIDVLL
                     GVSFGLAQQLLLIAPAAIVYMSVASTNSRLQARAAIAARWMLGLPPIVFGLLHLIGVR
                     VFARIVPHWMPFGNFWAVLTGIAFILAGVAICSGIRDVLAARLLALMLLLFEGLVEVP
                     PVFIRLHNQATWAAATYNLAAIGACWIFAEFLASRANREQIDSVGNVARPRPVAA"
     gene            5827..6858
                     /locus_tag="VHE33_07890"
     CDS             5827..6858
                     /locus_tag="VHE33_07890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HVT97415.1"
                     /translation="MAAPESPTESFDVLVIGAGPTGMACAIEAQRAGFRSVLIDKGCL
                     CNSLFHYPSHMTFFTTSELLEIGDIPFPSTNAKPSRNEALEYYRKVAMFYKLDVRQYQ
                     RVEQVSGSDLAFKIRTVDDFGRSNNFSTRKVVVATGYYDLPNLMNIPGEDLPKVRHYY
                     DDPHPYYAQDVLVIGGKNSAAIAALELWRHGARVTLVHRGPSLHRHVKYWIKPDIENR
                     IKNGEIAAHFNTAVTRIDPDTVTLQSEGGQPRTLKNHAVLAMTGYRPDFEFLKSLGVR
                     IEGENRCPACDPASLESNIPGIYLAGVIVAGDRTNEIFIENGRFHGRQIANDLRVKLT
                     SSAAEPLRV"
     gene            6971..8818
                     /locus_tag="VHE33_07895"
     CDS             6971..8818
                     /locus_tag="VHE33_07895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT97416.1"
                     /translation="MPRSIRGQFMAALILFEIIVVGIFTLLLLRQQQSEMTQRIHRRL
                     ESQAFEMASLASFAFTDPKPNSLAHVFAIATSSTSIGSAQITDLEGRNLASSDPANLG
                     KITLGPTERTFLPFPANPVIFRPAKGHWEIVAPIAVAGHTQAVAWISSNDHQDNLDLR
                     ALLRITALWAAFVFGACTLIAAILAQSITRPLGMLTRATRRLIQHPEEPSADFPLPVK
                     TSTEAAELTIAFNRMVAAIEDQRAGLNETLALLDSMLANAPIGFAFFDRKYRFVRVNQ
                     FLADMNGYSFALHLGRGVSGILPPPTAAKLERSIETVFQSGHPVRNLELTWEPEPRRS
                     RTWLVHLYPVHTETDTTRWVGAVLVDTTDQKRSEETLRRTEKLAATGRLAASIAHEIN
                     NPLEAVTNLLFLIRQEHLSPEASRFAELAQQEVTRVSEITQQTLRFYRQSTLPTLSNV
                     AELLDSVITLHLGRIQTLQIDLRRDYDRSLQLFCFAGELRQLFANFISNALDAMTPEG
                     GRLIIRTHAATLKSVPGIRISVADTGGGISQDARRHIFEPFFTTKEATGTGLGLWVSD
                     QIIRKHNGTVRVRSRSTGRTGTVFSIFFPASGVEPDPTSTETAGETVSS"
     gene            9257..11053
                     /locus_tag="VHE33_07900"
     CDS             9257..11053
                     /locus_tag="VHE33_07900"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HVT97417.1"
                     /translation="MLVRSRCLPALLLLATLPTFATAQKKKSAPAPAPAASTNPDIGP
                     WSEPQPAQEKLDLTMYQKIREEGLERSRIMQYASALSDDIGPRLTGSPNMAKANAWTR
                     DQLTAMGCVNAHLDDWGEFGMGWQQLNTWVRMTAPDTAVFIAQATPWSPATNGPVNAD
                     ATFVNIQSDEDFAKYQGKLAGKIVLLGEMRPVPPVDAALFERYSDKELADMANFPVEE
                     RANVALQQRLRMYMQRMKLTDKLIQFLADEKVAAVIRPSRDAEHGGGSGGTIFDDNGA
                     SMGRTPYLRGHEMKVPTVVMAIESYGRVYRLLEAHVPVKIEMDVETKFLGDHEHGYDT
                     IAEIPGTDPQLKDQVVMVGGHLDSWIAGTGATDNGAGTLVAMEVMRILTALHVQPRRT
                     IRIALWSGEEQGLFGSRGYAKEHFGSAPLSTAPDQAALPEFMRRAAGPLELKPEQRLI
                     SGYFNLDNGSGKIRGVYLQGNAAIAPIFQQWIEPLKDLGVTTLTMQNTGGTDHLSFDA
                     VGIPGFQFIQDPLDYESRTHHSNQDVYERLQAADLKQAATVEAIFVYNAAMRDQMLPR
                     KPVPHPELRQQEMSPIKNLFPDAVPEQTPPQQ"
     gene            11156..>11929
                     /locus_tag="VHE33_07905"
     CDS             11156..>11929
                     /locus_tag="VHE33_07905"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVT97418.1"
                     /translation="MDRFSKAPTPLTRRDLLKYGSAFGLSSLLAGCGSASKAIPGTPQ
                     SSCAKITDIEHVVIFIQENRSFDHYFGSYPGVRGFADKSAAFQQPWPSNAGNPPNGVL
                     LPFHMDSTQTNAACTHDISHDWVPQHQSWNNGAMDGFVTSRLAINAGDAVLSMGYYTR
                     ADLPYYYALADAFTLCDNYFCPVIGPTDPNRLYAMAASIDPDGKNGGPLLQTLVANRS
                     SFFGRLTYTTMPEQLQARGISWKVYTSPDQNILSGLFSDN"
BASE COUNT         2381 a         4446 c         2939 g         2163 t
ORIGIN      
        1 tacccgcgtc ccaactccgg gaaccttctc ccatcaggcg gagtctgtcc acccaggagg
       61 cgcgaatctc atgtcgatga ccgctggcag caaccgcacc ctcaacgccg accccaacgt
      121 caccccgctc atcgatgtcc tgctcgtcct gctcatcatt tttatggtga tcgttccggt
      181 cacgcccaga ggcctccggg ctgtcctgcc tcaacaaccc agccaggccc acccccaccc
      241 aacccgcacc atcgtcgtcg agatcctcgc cagcccgtcc ggccccgcct acagaatcaa
      301 ccagcaaaac atcgccgaca aggccgctct cggggccgaa ctgaaccgca tttactccat
      361 tcgcgccgag aaggtcctgt ttattcaggg cgaccccaaa ctcgactttg ccgccatcgc
      421 cgaagtcctc gacctgggcc gcgccctcgc catcgaccac atcggcatcc tcacacccca
      481 ccttcgtgcc ggctgattcc tgagcgcgac caccaacggc gcaattccat cggtaccggg
      541 atcaggtggt catcaccccg cgagtcgcgg cggattcccc aggagctttg cgccgcagga
      601 tggccgcgga aatcagagta atgatcaggc cgaccggcaa cggctccatg aacgtgtaag
      661 ccatgttgac caaaggattc tggtaaagct gctgcgaccg ctggatggcc gccacctgcg
      721 cggcgatcgt ggccgcgtca agtccggaag cctcgacccg gtgaatctgc gccgcaaagt
      781 agctgtccat gaagtggggc atgaagttga agtaaaggat ctcccacatg ccgacgtagc
      841 agaccgtggt gatcagcgtg atcaggatcc cgcaggcaaa agcgcgtccg aaggagattt
      901 gcccgcccag ggtgttgtcg cgatagctgc ggatgccgaa gtaaaccagc aggaacgacg
      961 ccaccatgat ggtgtagccc aaaaccatgc tgtggcccga gccaatcctg ttggcgatca
     1021 gtagcgatcc gcccatcagg acggagatca tgacgcccgc gataagaccg aaggtgagaa
     1081 cggttttctt catggaagtc tcctgtttcg aagtcagcga tggcgtcacc atgcggtaaa
     1141 acgccgtccg gtgtcgtcat tctttcgggt gatttttcgg gagggaaccg gaaaacatgc
     1201 tttcggatga cgcaattacg gcagtagtcc caattccttg ccgcgctgaa cggcctgcgt
     1261 gcgccggacc actcccagct tgtcgaacgc gcgcgcgcag tgagtcttca ccgtgttttc
     1321 ggaaacgaaa agctgagcgc cgatctcccg attgctgagc ccccgggcga ccagcgtcag
     1381 aatctcaagc tcgcgcgccg tgatgccgag gctctgctgc cgggccgcat cgggagtaaa
     1441 gggtccggcg gcgggagctt ccgccgggac cagcacctcc ctcaccacca ccgtttcgcg
     1501 aatgatctct cggctgcgcg tgatgcgaag ccccagccag atgccaaagc tcgcaaacag
     1561 gatcgcgatc agcgccccgt aaagctcgac ggagtgctca atcacgacga agcggtattc
     1621 ggtgtactga agggcggcga taagcaggcc ccccacgaca ccgaagatga gaacgtggcg
     1681 cttcatgatc ggattgtgaa agagtatatc ccgctccgct cctgactcac gcccctgagc
     1741 ttcgctcacg acggcgtcac tgacccgccc ctctctcctg cgcacacaaa gccagtccat
     1801 acgcaaccga ggtgaactca tgcccgcccc gaatcttctc cctgccaaac cgtcgcgtaa
     1861 agatccgccg caccgccggc accagcgacg ttcctccggt aagaaaaacc cgatcgacct
     1921 cgcccggact cactcccgtt tgccggatca gagaatccac ggcatcttca atctttccca
     1981 gatcctcagc aatccacgtt tcaaactcac ggcgtgtcag cggaacccgc agatccagac
     2041 cgccctcctg gaagcgaaac tccgcagatt cctgctgcga aagcgcgaac ttcagccgct
     2101 gcaccgcctg gtgcagctgg tatcccagat cctgctcgat caccgcgatc agcgcctcga
     2161 tcttctccgg ctccagcccc cggatgcgtg cgcctcgcaa aatctcgcgc acattgttcg
     2221 tgcgcaggaa cgagaggtag tgccagcgct cgagattcgc gtagatccac gccggcactg
     2281 ccggaagaat cttatccagc gatcgcgcct ctgaatgcga ccccaaagca ggcgacacca
     2341 gcttccgaac aatgcgcgca tcgaaggcgt ccccggccag tccgacgccg ctgtagccca
     2401 gcacctgacg ctggccgcgc acccgtgccc ccggccccac ccgcagcagc gagaagtcgc
     2461 ttgtcccccc gccaaaatcg ccaatcagga ttagctcatc gtggtccagt gtggactcgt
     2521 acgcataagc cgcacccacc ggctccagtt cgaattccac cgcctcgaag ccggcaatct
     2581 gaaatgcctc gcgtaaccgt gcgaccgcaa agtcgtcatc gtccgtcgtc tccgcgccca
     2641 cgaaccgcac cggccgtccc accaccgcgc gccgaatcgg cctcccgaac tgcttctccg
     2701 catgcttccg cagatcgctc agcatgcgcg agatgagttc ttccagccga tgccgccgcc
     2761 caaagacttc cgtcccggtc agcgtgcggc tcgaaaggtg cgacttcagc gattgcacca
     2821 gccgcccctt gtcgtcagcg tcgagatagc gctcaatcgc cgccggcccc gaccagctgt
     2881 gcgtccgctt cattccgccg gccgatgcga cctgttcgaa atacaacacc gaccgcgaag
     2941 aaagcgtctc cgccccggca aaggtgaacg atgccagttc cacccgtgac tctcccatca
     3001 cgagtgcaac agagctgttc gtcgtcccaa aatcgatacc ggcaacgctc aacggcgaca
     3061 ttcctatctc gcggcgcaga ggttttttcc aaagccgaac cgagcagccg cagcaagaag
     3121 gataaagcgt cgcgccaaat tgtttcagtt gcacgctccc gcgcgtgtcg cgatcctcag
     3181 tgatcaaccc tgcgaatcgg ccttcgtcga gagacctcgt ctcatcttcc gtagtctcgt
     3241 tagctcaatg cctttgcgag tcgaatcagt ccgtgccgta tgtcaggctc gttgaatccg
     3301 ccaaagccga gcagcaatgc atgcggatca ggacgtctca gggtgcaccg atcgacggca
     3361 agaacctcaa tgccctgggc agcggccagc ttttctgcct cttgtgaagt catttcattc
     3421 ctgagatacc cgatcgtgta cagccctgcc cgcacattcg agatctcaag caggcctccc
     3481 agatgttgca tggctccttc catcagagca gcgaggcgct ccgcatacaa attccgcatc
     3541 ctccgcagat gtcgcgccaa atgcccttca acgatgaagt cgcagagtac tgcctgatca
     3601 aaggatgaat tcctgaaatc ggttcgataa cggaacccca ggaaaccatc gatcaacggt
     3661 gcaggcaata ccacgtagcc cacccgcagg gccggaaaga gagtcttgct gaacgatcca
     3721 acgaagataa cgctcgaatg attgtcgagg ctttgaaggg cgggcacagg cggtccctca
     3781 aacctgtatt cgctgtcata gtcgtcttca attacaaaag cccccgaacg ggcagcccag
     3841 ctcagcagcg ccatccgccg ctccagcgac atcgttactc caagcgggaa ctggtgagcc
     3901 ggcgttacgt aggctcccat cgcatccgga caggctttaa ttccggcggc aaccgagagc
     3961 ccttcatcat cgaccggcac agcaaccatt ctggcttcgg cgctggtaaa tgcaatcctg
     4021 gctccgaaat atcccggatc ctccatccag atccgatcgc cccttttcaa cagcaaccgt
     4081 gccagcagat cgagcgcctg ttgtatccct gaaacgatca cgacctgttc cgctgtgcat
     4141 cgcaccccgc gggccgtccg caaatactcg gcgatcgcag cgcgcaatgg ccgataccca
     4201 ctccgatcgg cctccttctt taaccatgac ctgaaatgac gtgcccggtc tgccgcaata
     4261 cgtccccaaa tctcggacgg gaattcgctg atggctggga ccccgatgcg aaagggacga
     4321 atcttgtccg caaaagccag gtccacccag gattgcggcc gtttataggt cgaaaggaca
     4381 cgccgaatat aggccggtgt cgttgtggcc tgacgggccg gccggggcgg ttccacacga
     4441 ttgacccacg taccgaatcc aacgcgcgag ctgacatacc cctcggcctg taaacgctcc
     4501 agtacgctga cgactgtgcc ccgggagagt tcgtattgtc gggcgaaatc tcttgacgac
     4561 ggcagcctgg tgcccgccgc cagccggcca tcgagaatgg cagaacgcag ttccccatac
     4621 agccaatggg tcagggtctg atggcgcgac cggctcttga gagtgagctc gaacgagctg
     4681 acggctttgg gcatattcaa tggtccaata gatttggaga aaatggctct gttcgatgga
     4741 ccaccgcggt catactatca gttcctggat cgagacacgc attctcggaa ccgagtgccg
     4801 gcttctcgct gcgcgactca tgggagatct acagatgaaa gttggagttt ggttctatgg
     4861 tctgggcctc gctctcaccg gcatactcga cattgtgtgg cgccagttcg atgcctctca
     4921 tcaacccatc caatctctcg gcaaagtccc cgatcaaaac atactggcct gcatcgtggg
     4981 cgtgtggttg gtcgcggctg gcctggccat cctgtggcgg cgcagcgcaa ggtttggcgc
     5041 cgcggcatcc gccggtgcct gcctgctttt tgtgattttc tggctggctc ggtactacgc
     5101 cggaattcac gcgcttggct ggcgaatcga cgtcttactc ggcgtctcct tcggcctcgc
     5161 ccaacagctg ttgctgatcg ctccggcagc aatcgtctac atgtccgtcg cctcaacgaa
     5221 ttctcgcttg caggccagag ccgcaatcgc ggcacgctgg atgttaggat tgccgcccat
     5281 cgtcttcggc ctgctccacc tcatcggcgt tcgcgtcttc gcgcgcattg tcccgcattg
     5341 gatgccgttt gggaattttt gggcggtcct gacaggaatc gcattcatcc tggcaggagt
     5401 tgccatctgc tccggaatca gagatgtcct ggccgcaagg ctgcttgcgc tcatgctgct
     5461 tctctttgaa ggtctggtag aagtgcctcc tgtttttatc cgcctgcaca atcaggctac
     5521 ctgggctgcc gccacctaca acctcgccgc gatcggggca tgctggatat tcgccgagtt
     5581 tctggcaagc cgcgcaaacc gggaacaaat cgattctgtc gggaatgttg cccggccacg
     5641 cccggttgct gcgtgattga gggatgcgtc gcagggtggc ctgggatcgc accggctcgg
     5701 ttgatgagcg caggccaaat gcgctgattg acgagagatt ctggtggttg ggtgcatctc
     5761 cacagtccgt tccaccgttc ccacgctgga atcgaacttc cagcaatcat cgtctaatct
     5821 acaaacatgg cggcccccga gtcccccaca gagtccttcg acgtcctcgt catcggcgcc
     5881 ggccccaccg gcatggcctg cgccattgaa gcccagcgcg ccggcttccg ctccgtcctc
     5941 atcgataagg gctgcctctg caactccctc tttcactacc cctcgcacat gaccttcttc
     6001 accacctccg aactcctcga gatcggtgac atccccttcc cctccaccaa tgccaagccc
     6061 tcccgcaacg aagccctcga gtactaccgc aaggtcgcca tgttctataa actcgacgtc
     6121 cgccagtacc agcgcgtcga gcaggtctcc ggctccgatc tcgccttcaa gatccgcacc
     6181 gtcgacgact tcggccgctc caacaacttc tccacccgca aagtcgtcgt cgccaccggc
     6241 tactatgacc ttcccaacct catgaacatt cccggcgaag acctccccaa ggtccgccat
     6301 tactacgacg acccccatcc ttattacgca caggatgtgc tggtcatcgg cggcaagaac
     6361 tccgccgcca tcgccgccct cgagctctgg cgtcacggcg cacgcgtcac cctcgtccac
     6421 cgcggcccct ccctgcaccg ccacgtcaaa tactggatca agcccgacat cgagaaccgc
     6481 atcaaaaacg gcgagatcgc cgcgcatttc aacactgccg tcacccgcat cgatcccgat
     6541 accgtgaccc tccagtccga aggcggccag ccccgcaccc tcaaaaacca cgccgtcctc
     6601 gccatgaccg gctaccgacc cgatttcgaa tttctcaagt ccctcggtgt ccgcatcgaa
     6661 ggcgaaaacc gctgccccgc ctgcgatccc gccagcctcg aaagcaacat ccccggcatc
     6721 tacctcgccg gcgtcatcgt cgccggcgac cgcaccaacg aaatcttcat tgagaatggc
     6781 cgcttccacg gccgccagat cgccaatgac ctgcgggtga aattaacgtc atcggcggcc
     6841 gaacccctca gggtgtagga tcacaccaaa acccataaaa gatctgccag cgggaccgtg
     6901 cgaagctctc ccctctgaat gcagacaggc tcctgtcgga acacggttgt ccccaacgat
     6961 gtcgctgccc atgccacgct cgatccgcgg tcagtttatg gccgccctga tcctgttcga
     7021 gatcattgtt gtcggcattt tcacgctgtt gcttctccgc cagcagcagt ccgaaatgac
     7081 ccagcgcatc caccgccgcc tcgaaagcca ggctttcgag atggcctctc tcgccagctt
     7141 cgccttcacc gaccccaaac ccaactccct cgctcacgtc ttcgccatcg ccaccagcag
     7201 caccagcatc ggctctgccc agatcaccga tctggaaggc cgtaacctgg ccagcagcga
     7261 ccccgccaac ctcggcaaga tcacccttgg cccgactgaa cgcacctttc tgccctttcc
     7321 tgccaacccc gtcatctttc ggcccgccaa aggccactgg gaaatcgtcg cgccgatcgc
     7381 cgtcgctggc cacacccagg ccgtcgcctg gatctcttcc aacgatcatc aggacaacct
     7441 cgacctccgt gccctgcttc ggatcacggc gctctgggct gccttcgttt tcggcgcgtg
     7501 caccctcatc gccgccatcc tcgcgcagtc catcacccgt cccctcggca tgctcacccg
     7561 cgcgacacgc cgcctcatcc agcatcctga agagcccagt gccgatttcc cgctccccgt
     7621 caaaacctcc accgaagccg ccgagctcac catcgccttc aaccgcatgg ttgcggccat
     7681 cgaagaccag cgcgccggac tcaacgagac cctcgccctc cttgactcca tgctcgccaa
     7741 cgcccccatc ggcttcgcgt tcttcgaccg caaataccgc ttcgttcgcg tcaatcagtt
     7801 cctcgctgac atgaacggct actccttcgc cctccatctg ggccgcggcg tcagcggcat
     7861 cctcccgccc cccaccgccg ccaaactcga gcgctccatc gaaaccgtct tccagtccgg
     7921 ccatcccgtc cgcaatctcg aactgacctg ggaacccgag ccgcgacgct cccgcacctg
     7981 gctcgtccac ctctatccgg tccacaccga aaccgataca acgcgctggg tgggcgctgt
     8041 cctcgtcgac accactgatc agaagcggtc cgaagagact ctccgccgca ccgagaagct
     8101 cgccgccacc ggtcggctcg ctgcttccat tgcgcacgag atcaataatc ccctcgaggc
     8161 cgtcaccaat cttctcttcc tcatccgcca ggagcatctg tcgccggaag cctctcgctt
     8221 tgccgaactg gcccagcagg aagtcacccg cgtctcggag atcacccagc aaaccctccg
     8281 gttctaccgc cagtccactt tgcccacact ctcgaacgtg gccgaactcc tcgactccgt
     8341 catcaccctt catctcggcc gcattcaaac ccttcagatc gacctgcgcc gcgattacga
     8401 ccgcagcctg cagcttttct gcttcgccgg cgaactccgc cagctcttcg ctaacttcat
     8461 cagcaatgcg ctcgatgcaa tgacgcccga gggcggccgt ctcatcatcc gcacccacgc
     8521 cgccaccctg aaatccgttc ctggcatccg tatctcggtt gccgacaccg gcggcggcat
     8581 ctcccaggat gcccgccgcc acatcttcga acccttcttt accaccaagg aagccacggg
     8641 taccggcctc ggcctctggg tcagcgatca gatcatccgc aaacataacg gaacggtgcg
     8701 cgtccgcagc cgttccaccg gccgcaccgg aacggttttc agcatcttct tccccgcctc
     8761 aggcgtcgag cccgacccaa cctccaccga aacggccggc gaaaccgtgt cctcctgagc
     8821 aaatcgctcg gcctcatccc cttgaggtaa tcgccgcagc cctcaacgtt ccagcggccg
     8881 tgactgtgca gttgaatgac ccgcacccgt aacgcgaact cccaaaaaca agaagagcgc
     8941 caggttcacc ccacagcaag cagaagactc tcccgcaacc ttcgaaatca acccctgttt
     9001 tcaacccttc agcacgaaag tcctccctcc atcccaaatc ggaaccagca accctcacac
     9061 cgcgccctgt gcacgcggcg agccacacct ccaatcacca cgccgcaacc atccgcagtc
     9121 acgcgagcgc aaggctcctt cgccagccct cgcaccgcta cccgtaccgc ttttcctgta
     9181 ccctagctcc ctgagccctg agccctgagc tctgagctct gcaccctgta ccactttccg
     9241 gaggaaccac acgcaaatgc tcgtccgctc tcgatgtctc ccagctctcc tgcttctcgc
     9301 cacactcccc accttcgcta ccgcgcaaaa aaagaaatcc gcccccgccc cagcccccgc
     9361 cgcctccacc aatcctgaca tcggtccctg gtccgaaccc cagcccgccc aggaaaaact
     9421 cgacctcacc atgtatcaga agatccgcga agagggcctc gaacgctccc gcatcatgca
     9481 atacgcctcc gccctcagcg acgacatcgg cccgcgcctc accggctcgc ccaacatggc
     9541 caaagccaat gcctggaccc gcgatcagct cactgcgatg ggctgcgtca acgcccacct
     9601 cgacgactgg ggtgaattcg gcatgggctg gcagcagctc aacacctggg tgcgcatgac
     9661 cgcgcctgac accgccgtct tcatcgctca ggccacgccc tggtcgcccg ccaccaacgg
     9721 ccccgtcaac gccgatgcga ccttcgtcaa tatccagtcc gacgaagact tcgccaaata
     9781 ccagggcaag ctcgccggca aaatcgtgct cctcggcgaa atgcgccccg tcccgcccgt
     9841 cgacgccgcc ctcttcgagc gctactccga taaggaactc gccgacatgg ccaacttccc
     9901 cgtcgaagag cgcgcgaatg tcgccctgca gcagcgcctt cgcatgtaca tgcagcgcat
     9961 gaagctcacc gacaagctga ttcagttcct cgccgacgag aaggtcgcgg ccgtcatccg
    10021 tccctcccgc gatgccgaac acggcggcgg ctccggcggc accatcttcg acgacaacgg
    10081 cgcctccatg ggccgcactc cctacctccg cggccacgag atgaaagtcc ccaccgtcgt
    10141 catggccatc gaaagctacg gccgcgtcta ccgcctcctc gaagcccacg tccccgtcaa
    10201 aatcgagatg gacgtcgaga cgaaattcct gggcgaccat gagcacggtt acgacaccat
    10261 cgccgaaatt cccggcaccg acccgcaact caaagaccag gtcgtcatgg tcggcggcca
    10321 tctcgacagc tggatcgccg gcaccggcgc caccgacaac ggtgctggca cgttggtcgc
    10381 tatggaggtc atgcgcatcc tcaccgccct ccacgtccag ccccgccgca ccatccgcat
    10441 cgccctctgg tccggcgaag agcaggggct cttcggttca cgcggctatg cgaaggagca
    10501 cttcggctcc gcgcctctgt ccaccgcacc cgaccaggcc gctctgcccg agttcatgcg
    10561 ccgcgccgcc ggcccgctcg aactcaagcc cgagcagcgc ctcatctccg gctacttcaa
    10621 cctcgacaac ggcagcggca agattcgcgg cgtctacctc cagggcaacg ccgccatcgc
    10681 ccccatcttc cagcagtgga tcgagccgct gaaggacctc ggcgtcacca ctctcaccat
    10741 gcagaacacc ggcggcaccg atcacctctc cttcgacgcg gttggcatcc ccggcttcca
    10801 gttcatccag gaccccctcg actacgagtc ccgaacccac cactccaacc aggatgtcta
    10861 cgagcgcctc caggccgccg acctcaaaca ggccgccacc gtcgaagcca tcttcgtcta
    10921 caacgcagcc atgcgcgacc agatgctccc gcgcaaacct gtcccgcatc cggaactccg
    10981 ccagcaggag atgtcgccga tcaaaaacct cttccccgac gccgtccccg aacaaacccc
    11041 accccagcag tagctatccc tcaacggctg tcctcctgag cgatcgcgcg gaaccaacgt
    11101 tccgcgcgtc gcgaaggacc tcgtgttttc caaccccatc cgcatccaag ctggaatgga
    11161 tcgtttctcc aaggccccca cccccctgac ccgccgcgac ctcctcaaat acggctccgc
    11221 cttcggcctc tcctctctcc tcgccggttg cggatccgcc agcaaggcta tccccggaac
    11281 cccacaaagc tcctgcgcca aaatcaccga catcgagcac gtcgtcatct tcatccagga
    11341 aaaccgctcc ttcgaccact acttcggcag ctaccccggc gtgcgcggct tcgccgacaa
    11401 aagcgccgcc ttccagcagc cctggccctc caacgccggc aatcccccca acggcgttct
    11461 cctgcccttc cacatggatt caacccaaac caacgccgcc tgcacccacg acatctccca
    11521 cgactgggtc cctcagcacc aaagctggaa caacggcgct atggatggct tcgtcacctc
    11581 ccgcctcgcc atcaacgccg gcgacgccgt cctctccatg ggctactaca cccgcgccga
    11641 cctgccctac tactacgccc tcgccgacgc cttcaccctc tgcgacaact acttttgccc
    11701 cgtcatcggc cccaccgatc ccaaccgcct ctatgccatg gccgcctcca tcgaccccga
    11761 cggcaaaaat ggcggcccgc tcctgcagac cctcgtcgcc aatcgctcgt cgttcttcgg
    11821 ccgcctcacc tacaccacca tgcccgaaca actccaggcc cgcggcatct cctggaaggt
    11881 ctacacttcg cccgatcaga acatcctcag cggcctcttc tccgacaac
//
LOCUS       DASZYN010000304         4199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1038421, whole genome shotgun sequence.
ACCESSION   DASZYN010000304 DASZYN010000000
VERSION     DASZYN010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038421"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VHE33_07910"
     CDS             complement(<1..455)
                     /locus_tag="VHE33_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVT97419.1"
                     /translation="MDRRDFLRASAAAAAASAFSGSAAGQKAAPAHRPESPNMIYREL
                     GRTGERVSAIGLGGFHVGKQSDAQESVRLIRSAIDRGITFLDNCWDYNAGASEVRMGQ
                     ALQDGYRQRAFLMTKIDGRDAKTAALQIEESLDRLKTDVIDLMQFHECIR"
     gene            628..1107
                     /locus_tag="VHE33_07915"
     CDS             628..1107
                     /locus_tag="VHE33_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97420.1"
                     /translation="MGKINIGRWVVGGLVCGIVLNCLGYLVDGLLLASQWNQNLVRLG
                     RPELNTSQMIWFNLIGFALGLLAVWIYAAIRPRFGAGVGTAAIAGIAVWVAASVLPNA
                     SMMWVTGLFNNHLTLYTTLGALVELVVGTIAGAALYREESATVAAAAPPARQTVGAA"
     gene            1477..2742
                     /locus_tag="VHE33_07920"
     CDS             1477..2742
                     /locus_tag="VHE33_07920"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HVT97421.1"
                     /translation="MATVTLEQEINPWEAQAARFDFAARKLNLDEGLWRVLRYPSREV
                     IVHFPVGMDDGRIEMFTGYRVQHSQARGPGKGGIRYAPDVTLDEVRALASWMTWKCAV
                     VNIPFGGAKGGVICDPKKMSQGELERMTRRYTSEIIEFIGPEKDVPAPDVNTNEQTMA
                     WIMDTYSMHMRQTVTAVVTGKPLNIGGSRGRREATGRGIQIICDEALKYLGIQRDGCR
                     VIIQGFGNVGSNAARLMQERGYKIIGIGEYDGGLYNPNGIEIGSLEEHKLRNGTVLGF
                     RGAEPMPTAELLTTECEILIPAATENVITSRNAENIRARIVCEGANGPTTAVADEILA
                     DKKIFIVPDILANAGGVTASYFEWVQDRQGYFWKESEVNERLASILAESFDDVTRYAE
                     AHAVNNRIAAYMLAIDRVAFTIKQRGIYA"
     gene            complement(2888..3799)
                     /locus_tag="VHE33_07925"
     CDS             complement(2888..3799)
                     /locus_tag="VHE33_07925"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HVT97422.1"
                     /translation="MRKLLAYGAIYILWGGSFLAIREVVAVLPPFFAAGFRFTVAGLI
                     LILWSHFTGPVQLSARQFLNATLLGFIMFACLYSGLFWAETRIPSGVAALVSALIPLW
                     IYAGEVLFLRTQRATLLASGGIAAGFAGIALLALRHGPQVHAHGYARAVAVTVAGTLC
                     WGIGTILSRRLTLPSPQKCNAGWQMYTGGMFLMMAAFAAGERQLLPRMSVMLSAHVVL
                     SMGYLILAASIVAYTSYVWLIAHDSPTRVSSYAYVNPVIAVALGIALAGEHLSRGQAV
                     GAVLVIFGVAATLMGKRRAEKRLAESD"
     gene            3981..>4199
                     /locus_tag="VHE33_07930"
     CDS             3981..>4199
                     /locus_tag="VHE33_07930"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HVT97423.1"
                     /translation="MATSNIKPAVPKSGAQGFQPYVPSSETRPELTFRAIFLGCLFGL
                     IFGAVTVYVGIEAGLTVAASIPIAVLSIS"
BASE COUNT          822 a         1432 c         1231 g          714 t
ORIGIN      
        1 cgaatgcact cgtggaactg catcaggtcg atcacgtcgg tcttgagccg gtcgagcgat
       61 tcctcgatct gcagagccgc ggttttggcg tcgcgcccgt cgatcttcgt catcaggaac
      121 gcgcgctgcc gatagccgtc ctggagcgcc tgccccatgc gaacctcgct cgcccccgcg
      181 ttgtagtccc agcagttgtc caaaaacgtg atcccgcggt cgatcgccga ccggatcagg
      241 cggacgctct cctgcgcatc ggactgcttg ccgacgtgaa agcctccgag cccgatggca
      301 gacacccgtt ctccagtgcg ccccagctcc cggtaaatca tgttgggcga ttcggggcgg
      361 tgggcaggag ccgccttttg cccggcagcg gagccggaga acgccgaagc cgctgcagcg
      421 gcggcgctgg cgcgaaggaa atcccgacgg tccatcctgg tcaaatccat attgactgag
      481 attgtcgccc agaaaggatg gatgtggggc cgaatcgagt cgtcggtccc ctcgcggagc
      541 gggcgttttt gcccaaaatt gggtgcaaat ttccattgaa ggtccctcac agggcgagta
      601 cactgcctca tcccccaagg aggctgtatg ggtaagatca acatcggccg ttgggtcgtg
      661 ggcggtcttg tgtgcgggat cgtcctcaat tgccttggat acctcgtgga tggcctgctg
      721 ctggcatcgc agtggaacca gaatctggtc cgcctgggac gtcccgaact caataccagc
      781 cagatgattt ggttcaacct gatcgggttc gcgctcggcc tgctggcagt ctggatctat
      841 gccgcgatca ggccccggtt cggcgccggc gttggcacag cagctatcgc gggcatcgcc
      901 gtctgggtgg ccgcttccgt gctgccgaac gcatccatga tgtgggtgac aggcctgttc
      961 aacaaccacc tcaccctcta cacaacactc ggcgcgctcg ttgaactggt cgtcgggacc
     1021 atcgctggag cggctctgta tcgggaggag tccgcgacgg tggcggcagc agcccctccg
     1081 gcacggcaga cggtcggggc agcctaaatc ggcgtgaatc gaacctgagc ccgtctgcga
     1141 agcctcttcc aacggcttcg caggccggtt gcaggacacg gcgtgctgcg ggaacgcccg
     1201 ccggcgcaag gagtgtgccc cccgcgagcc ctccgggctc gggtgtccag cccggaaacc
     1261 tccccggaat cggcccgcat ctcccacagg tgaccttttc aagggctccg ggtgatgccc
     1321 cacaagggta acctctcccg gcccgacgag ggttgcgggc gtctggtccc tgggcctata
     1381 ctcggaccgt ttggcccgga tgcgggttca atccgaaatg aaccggatgg aaccattttt
     1441 ttgcagggcc ggtcaggatg agttggagat tctccgatgg cgacggtaac gctggaacag
     1501 gaaattaacc cctgggaagc ccaggcggca cggttcgatt ttgcggcgcg caaactcaat
     1561 ttagacgaag gcctctggag ggttctccgc tatccctccc gcgaagtcat cgtgcacttc
     1621 cccgtcggca tggacgacgg ccgcatcgag atgtttaccg gctaccgcgt gcagcattcc
     1681 caggcccgcg ggcccggcaa gggcggcatc cgctacgcgc ccgatgtcac cctcgacgaa
     1741 gtgcgcgccc tcgccagctg gatgacctgg aagtgcgccg tcgtcaatat tcccttcggc
     1801 ggcgccaaag gcggcgtcat ctgcgatcca aagaagatgt cccagggcga gctcgagcgc
     1861 atgacccggc gctacacatc ggagatcatc gaattcattg ggccggagaa ggatgtgccc
     1921 gcgcccgacg tcaacaccaa cgagcagacc atggcctgga tcatggacac ctactccatg
     1981 cacatgcgcc agaccgtgac cgccgtggtc accggcaagc cgctgaacat cggtggatcg
     2041 cgcggacgcc gcgaggcgac cggacgcggc atccagatca tctgcgatga ggcgctgaag
     2101 tatctcggca tccagcgcga cggctgccgc gtcatcattc agggtttcgg caacgtcggt
     2161 tcgaacgcgg cgcggctgat gcaggagcgc ggctacaaaa ttatcggcat cggcgagtac
     2221 gacgggggcc tctacaaccc caacggcatc gagatcggct cgctcgaaga gcacaagctg
     2281 cgcaacggaa cggtcctggg cttccgtgga gccgagccca tgccgaccgc cgaactgctg
     2341 accaccgagt gcgagattct cattcctgcc gcgacggaga acgtcattac cagccgcaac
     2401 gccgaaaaca tccgcgcgcg catcgtgtgc gaaggcgcga acggccccac caccgccgtc
     2461 gccgacgaga tccttgccga caagaagatt ttcatcgtcc ccgacatcct cgcgaatgcc
     2521 ggcggcgtca ccgcttccta cttcgaatgg gtgcaggacc gccagggcta cttctggaaa
     2581 gagtcggaag tcaacgagcg cctggccagc atcctcgccg agagcttcga cgacgtcacc
     2641 cgctacgctg aagctcatgc cgtcaacaac cgcatcgcag cctacatgct cgccatcgac
     2701 cgcgtagcct tcaccatcaa gcagcgaggc atttacgcct gagggcagcg aacagggatt
     2761 agcgatgatg attagcgaaa gactgttagc aagcagctgt tggcaagtga ggatacgcta
     2821 atcgcctctc gccatccgtt ttccgctaag cgctgttcgc taaacgctgt tcgctagccg
     2881 ctgttcgcta atcgctctcc gctaaacgct tctccgctcg ccttttcccc atcaacgttg
     2941 ccgccacccc gaagatcacc agcaccgctc ccaccgcctg cccccgcgaa aggtgctccc
     3001 ccgccagcgc aatccccagc gccaccgcga tcaccggatt cacatacgca taactcgaca
     3061 cccgcgtcgg cgagtcatgc gcgatgagcc atacatagct cgtatacgcc acaatcgacg
     3121 ccgcaagaat caaatacccc atgctgagga ccacatgcgc gctcagcatc accgacatgc
     3181 gcggcagcaa ctggcgctcc ccggcggcga acgcggccat catcaggaac atgccgccgg
     3241 tatacatctg ccaccccgca ttgcatttct gcggactcgg cagcgtcagc cggcgcgaaa
     3301 gaatcgtccc gattccccag caaagcgttc cggcgaccgt caccgccact gccctcgcgt
     3361 acccgtgggc gtgcacctgc gggccatgcc gcagcgccag caacgcgatt cccgcaaacc
     3421 cggccgcgat cccgcccgac gcaagcagcg tcgcccgctg cgtccgcagg aacagaacct
     3481 cgcccgcgta aatccacagc gggatcaaag cggagaccag cgccgcgacg cccgacggaa
     3541 tccgcgtctc cgcccagaac agccccgaat agaggcaggc aaacatgatg aacccgagca
     3601 gcgttgcgtt caggaactgc cgagcactca gctgaaccgg accagtaaag tggctccaga
     3661 gaatcaggat caggccggca acggtaaagc gaaatcccgc cgcaaaaaac ggcggcagca
     3721 ctgcgacgac ctcgcggata gcaaggaacg aaccgcccca caggatgtaa atggcgccat
     3781 aggccaggag tttgcgcaca ggtgaaaaca gggtacaggg tgcagcagtc agggtacagg
     3841 gctagccgta cgctgtgccc tacgccgtgt gccttgtgcc ctgcgccctg tgctctgtgc
     3901 cctgtgctct gcaccctgcc ccctcctgcc ccgacactgg tacactcagg cttccccaaa
     3961 accgcttccg ggggtatcca gtggcgacga gcaacatcaa gcccgcagtt ccgaagtcag
     4021 gcgcgcaggg attccagccc tacgtgccct ccagcgagac gcggccagag ctcaccttcc
     4081 gcgccatctt cctcggctgc ctcttcggcc tcatcttcgg cgcggtcacc gtctacgtcg
     4141 gcatcgaggc cggcctcacc gtcgccgcct ccatccccat cgccgtcctc tccatcagc
//
LOCUS       DASZYN010000305         6333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_203629, whole genome shotgun sequence.
ACCESSION   DASZYN010000305 DASZYN010000000
VERSION     DASZYN010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203629"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VHE33_07935"
     CDS             <1..541
                     /locus_tag="VHE33_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97424.1"
                     /translation="PLRSALLKKMQKQIERPGMGPEELRTWLAQLTPAELGVDESADP
                     VLARFQVSLFTEASGTQIFSTTFAQWATREVLRRAQALTVIARFAPRQRQRPMNELLS
                     GNTAAAELDPAGSLIDADMAAWYHWVNQQRLPGYEQSGFLAWFEDHNQAVAIGPTIPR
                     GVQSDSSLDLAELLAFVTG"
     gene            675..2621
                     /locus_tag="VHE33_07940"
     CDS             675..2621
                     /locus_tag="VHE33_07940"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HVT97425.1"
                     /translation="MPQRSSWFGRVIAATLLLCSASLGHGQVAAGSAPIVVQGLGKGT
                     ASLSGPWRFHLGDNPAWAAPGFDDSGWEKVTADLPWGQQGHDRYTGYAWYRCRLALTP
                     APGVSPQFSLLLEHVDDAYEIYWNGRLIGHNGKLDPYRIWYYYYSEKAQTFALGPGQS
                     GVLAFRVWKAPLFSDDSGQGGGFESAPLIGSPEAIAAVRAALDYQWLSGRQFLFGQNL
                     IYALIALVSFLVWLRHRSQWLLFWMMAFTAAPVIILLLLGSGLHWPYLFVIGAAQPFY
                     SIQDEIALWFLLLWLLPLRESRRLCRLTRILAWVSFTTGLLDGAVLAISWGPRWIGPA
                     QIVDGVSAAIYTLLDAYPLILIAAAFPLRRRFDSARRMLAALAFLYGMVAVVLDASKQ
                     GRQFTNWTLATKLEAPLFTLGGSAISLHTLAGAFLLVALVYAVTSRLREDQRHKEALE
                     REKIELTRTRDEMRHFAENDDLTGLSNHRIIAERLTEEVERSRRQQAPLSVILADIDH
                     FKNINDTFGHVVGDRVLKEVAAIFRQSVRPYDSVGRYGGEEFLIILPGSDFESASTRA
                     EQLRRAVQSAHIMDGETQVRVTSSFGVASGFPGKDEAETVIRIADAALYEAKRNGRNR
                     VVVTEVSSPEDAPLPAAGGSQTPA"
     gene            complement(2647..2991)
                     /locus_tag="VHE33_07945"
     CDS             complement(2647..2991)
                     /locus_tag="VHE33_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97426.1"
                     /translation="MDSLQDYLCWVDEVVLPIAFQWIEEHKPEVYANVPDELKDDVGV
                     AVAAAFGLVKDLPVYKQGRERCKAHDWAEKAGTVEKSSMNPVWAGITRKIRRGTREAF
                     AEQSQVLDGTIP"
     gene            complement(3440..3526)
                     /locus_tag="VHE33_07950"
     tRNA            complement(3440..3526)
                     /locus_tag="VHE33_07950"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3490..3492),aa:Leu,seq:cag)
     gene            3752..3985
                     /locus_tag="VHE33_07955"
     CDS             3752..3985
                     /locus_tag="VHE33_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97427.1"
                     /translation="MTVPLQPKSIPEQQAKEIRRLSHDLSNALEIVLQTSFLLGTVPL
                     DDDAKKWHSMLDNGVQQAAEINRQLREFVRGNS"
     gene            complement(4001..4513)
                     /locus_tag="VHE33_07960"
     CDS             complement(4001..4513)
                     /locus_tag="VHE33_07960"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HVT97428.1"
                     /translation="MANHNRNWRAAAFRSGAWVALSFAALALNMVYAAEAVQYPGDFR
                     RWVHIGTGVILPGTSPQLKSEEGMHHIFANAKAVEGYASGNFTDGSMIVYELREAQQK
                     NGVIFEGERRRVDVMIRDSRHYSGTGGWRFERFWGNDAAHNAIQDAGASCFQCHSSAQ
                     THGFVFSQLH"
     gene            complement(4558..5373)
                     /locus_tag="VHE33_07965"
     CDS             complement(4558..5373)
                     /locus_tag="VHE33_07965"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVT97429.1"
                     /translation="MFRIHKPALPLSRYVESLWYASTSGMAPSRQRVYPDGAMALVIH
                     LEKPTATYFVGEETRSIGVPLLAGPWSQSFQIDPSLSTGVVGVVFRPGAARMFFPVAA
                     HELHNIDIPLQELYPSEADRLLNEVCSAAGEEARLRVVEEYLRQRLMRAAPIPPAVNY
                     AVRQLSSAGGVRRVRAIQRETGLSHTRFIQLFREHVGLTPKLFCRVRRFRALLNRMEK
                     GEPVSWAELAADCGYFDQAHMIRDFRAFAGATPGEFSRLPEAASRSSASPASS"
     gene            5551..5902
                     /gene="ssrA"
                     /locus_tag="VHE33_07970"
     tmRNA           5551..5902
                     /gene="ssrA"
                     /locus_tag="VHE33_07970"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            6021..6329
                     /locus_tag="VHE33_07975"
     CDS             6021..6329
                     /locus_tag="VHE33_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97430.1"
                     /translation="MPKITTRVKDLPGWMPRPIGLPSRFAPSDSDALGEATVEHILRV
                     MDDRIVFTCKYKGRRIDFDFSTEDRKTSDKVAEILRSHREKSLASIGTIELPADLLAA
                     "
BASE COUNT         1214 a         2060 c         1788 g         1271 t
ORIGIN      
        1 gcccctgcgt tccgctcttc tcaaaaaaat gcaaaagcaa atcgagcgcc cggggatggg
       61 acccgaggag ctccgcacct ggctcgccca gcttacgccc gcggagcttg gagtggacga
      121 atctgccgat ccggtcctcg cccgttttca ggtcagcctc tttaccgaag cctcgggaac
      181 gcaaatcttc agcaccacct tcgcgcagtg ggccacccgg gaggtgcttc gccgtgccca
      241 ggcgctgacc gtcatcgctc gcttcgcgcc gcgccagcgg cagcggccca tgaacgaatt
      301 gctgtccggc aacacggccg cggctgaact tgatcccgcc ggttcgctca tcgatgccga
      361 tatggccgcc tggtatcact gggtcaatca gcagcgactt ccaggctacg agcagtccgg
      421 attccttgca tggttcgagg atcacaacca ggccgtagcc atcggtccga cgataccgcg
      481 tggcgttcaa tccgattcgt cgctggatct ggccgagttg ctcgccttcg taaccggata
      541 ggcgagtctc tcagggggtc ctcattccca ccgcatgggc atgatgtgcc ggccgtccgg
      601 tagtttgaga acagtgacct tacgcccctg atccagccag gatgcacggt ataatcccct
      661 gcgtatcctc cccaatgccc cagcgttcct catggttcgg ccgtgtgatt gctgcgacgc
      721 tgctgctctg ttcggcctcc ctgggtcacg ggcaggttgc ggcaggcagc gctcctatcg
      781 tcgtccaggg cctgggcaag ggaaccgctt ccctcagcgg tccctggcgg ttccatctcg
      841 gagacaatcc cgcatgggct gcgcctggct tcgacgattc cggctgggaa aaggtgaccg
      901 ccgaccttcc ctggggccag cagggccacg accggtatac cggctatgcg tggtatcgct
      961 gccgcctcgc tctgaccccc gctcccggtg tttctccgca attttcttta ttgctggagc
     1021 acgtcgacga cgcctacgaa atctactgga atggcaggct gattggccac aacggcaagc
     1081 tggacccgta tcggatctgg tactactact actccgagaa agcgcagacc tttgcactcg
     1141 gtcctggaca aagtggcgtt ctggccttcc gggtctggaa ggcccccctg ttctccgacg
     1201 attccggcca gggcgggggt ttcgagtccg ctccactcat cggcagtcct gaagcgattg
     1261 ccgccgtcag agccgcgctc gactaccagt ggctgagcgg gcggcaattc cttttcggcc
     1321 agaacctcat ctacgcactc attgcgctgg tgagctttct ggtctggctg cgccatcgca
     1381 gccagtggct gctgttctgg atgatggcct ttacggcggc tcccgtcatt atcctgctgt
     1441 tactggggag cggtcttcac tggccttacc ttttcgtcat aggcgcggcc cagccttttt
     1501 attccatcca ggacgaaatt gccctgtggt ttctgcttct gtggctgctt cctctccgcg
     1561 agagccgcag attgtgccgg ctcacacgca tccttgcatg ggttagcttc accaccggtt
     1621 tgctcgacgg ggcggtgctg gcgatcagct ggggcccgcg ctggattggg ccagcgcaga
     1681 tcgtcgacgg cgtatccgcc gcaatctata ccctgctcga tgcgtatccg ctaatcctga
     1741 tcgccgcagc tttccctttg cgcaggcgct tcgattccgc tcgacgcatg ctcgcagccc
     1801 tggcttttct ttatggcatg gtggccgtcg tcctcgatgc ctctaaacaa ggccgccagt
     1861 tcaccaactg gactcttgcc acgaagctgg aagcgcctct gttcactctc ggcggcagcg
     1921 ccatctcgct gcatacgctc gccggcgcgt tcctgctggt cgccctcgtg tacgccgtga
     1981 ccagcaggct gcgcgaagac cagcgccaca aggaagctct ggagcgggag aagatcgaac
     2041 tcacccgcac tcgcgacgag atgcgtcact ttgccgaaaa tgacgacctg accggcctgt
     2101 cgaaccatcg catcattgcg gaacgcctca ctgaggaagt ggagcggtcg cgccgccagc
     2161 aggcaccgct cagcgtcatc cttgccgata tcgaccattt caagaacatc aacgatacct
     2221 tcggtcatgt ggttggcgat cgtgtgctca aagaagtggc tgcgatcttc cgccagtcgg
     2281 tgcgccccta cgattcagtg ggccgctatg gaggcgagga attcctgatc atccttcccg
     2341 ggtctgactt tgaaagcgcc agcactcgcg ccgagcaact gcgccgcgcc gtgcagagcg
     2401 cgcacattat ggatggcgaa acccaggtgc gagtcacctc cagcttcggc gtagcctccg
     2461 gtttcccggg caaagacgag gcggagacgg tgattcgaat cgccgacgct gccctctatg
     2521 aagccaaaag aaacgggcgc aatcgcgtcg tcgtcactga ggtcagttcg cccgaagacg
     2581 cgcccttgcc cgccgccgga ggcagtcaaa cacccgcgta gcgccggaac attcgtgccg
     2641 ttcggctcag ggaatcgttc cgtccaggac ctgcgattgc tcggcgaatg cctcccgggt
     2701 gcccctgcgg atcttcctgg taatgccagc ccacaccgga ttcatgctgg acttctccac
     2761 ggtcccggct ttctccgccc agtcgtgggc cttgcaccgc tcgcggcctt gcttgtaaac
     2821 aggcagatct ttcaccaggc cgaacgcggc cgcaaccgcc acgcccacgt cgtccttcaa
     2881 ctcgtcgggt acattcgcgt agacctcggg cttgtgttcc tcgatccact gaaacgcaat
     2941 ggggagcacc acctcatcca cccagcacag gtaatcctgc agggaatcca ttgcggcagc
     3001 ccacgaaggg tgcagctccc cgtccgggcg tctctcctct tttctgcaac acggcatgta
     3061 gacacaccct cttttcgtgg gcgacatgag cactttcctt tcctgaatga ggtttgcgcg
     3121 ttactgctat cttccgataa aaggcggcga gttcggcgga atcccctcac tcgaacacgt
     3181 ccccagcaac ttattcagca acaggagacc gcgatttccg gtcctctgtt cggccgggtg
     3241 tccgcgacgt gctctttttc ggtcccgtag ctgggggatg cgggttttaa gactctcacg
     3301 aatagaccgg cggccaacgg gcaccagccg gggtcataca cagccgacag ccttctgatg
     3361 gatggggcga taccgggatt caccatgctg caggttttcg ctttgcttaa gcaatgaaaa
     3421 cggagccggt tgaaacagtt ggtgccgaag aagggactcg aacccccaca cccttgcggg
     3481 tacatggacc tgaaccatgc gcgtctgcca attccgccac ttcggcacgg agagagttca
     3541 gcgcggcacc gggctcatga ccgaacaaat tcgagcagga gcgggcgcac caaacgggca
     3601 gtgtcgcaat tctatcgtat tccgcagccc gccgcgagcc tgtgttcgcg ctggaccggc
     3661 agcaactcct ggtgttacaa aggcatccac ccgtgtcaac ccgcgcacca cgcgctcagg
     3721 cccccatctt ctgtcaggat tattcttctc aatgaccgta ccgttgcagc ctaagtcgat
     3781 ccccgaacag caggccaaag agatccgacg cctctcccac gacctgagca acgcccttga
     3841 aattgtcctt cagaccagct tccttctggg cactgtgccc ctcgatgacg atgctaaaaa
     3901 gtggcacagc atgctcgaca acggcgtcca gcaggctgcc gaaatcaatc gccagctgcg
     3961 ggagtttgtc cgcggcaaca gctagtttcc ggccatccga tcaatgcagc tggctgaaga
     4021 cgaatccgtg tgtctgcgca ctggagtgac actggaagca cgaagcgccc gcgtcctgga
     4081 tggcgttgtg cgcggcatcg tttccccaga agcgctcgaa tcgccatcct cccgtgccgc
     4141 tgtaatgcct cgaatcccgg atcatcacgt ccactctgcg ccgctcgcct tcgaaaatga
     4201 ccccgttttt ctgctgagcc tcgcgcagct cgtacacgat catggaaccg tcggtgaagt
     4261 tgccggatgc gtagccctca acggccttcg cattcgcgaa gatgtggtgc atgccttctt
     4321 cacttttcag ctgtgggctg gtcccaggca ggatgacccc cgtccctata tggacccagc
     4381 gtcgaaagtc gccgggatac tgaacggctt ccgccgcata caccatgttc agcgccaggg
     4441 ccgcgaatga gagcgcgacc cacgcgcccg agcgaaacgc cgccgcgcgc caattgcgat
     4501 tgtgatttgc catgagggca ttctgcgcgt caccgaaacc cgcgtattgg aaaaaattca
     4561 actcgacgcg ggactcgcgg aactccggct cgccgcctct ggcagcctgc tgaactctcc
     4621 cggcgtggcg cccgcaaacg cccgaaaatc gcggatcatg tgcgcctgat cgaagtaccc
     4681 gcaatcggcc gccaactcgg cccagctcac cggctcaccc ttctccatcc gattcagaag
     4741 tgcgcggaat cgccgaaccc ggcagaacag cttcggcgtg agtccgacgt gctcgcgaaa
     4801 cagctgaatg aaacgggtgt ggctcaggcc cgtctccctt tgaatcgccc gtacgcggcg
     4861 tactccgcca gcactcgaca gttgcctgac ggcatagttc accgcggggg gaatcggcgc
     4921 ggcgcgcata agcctttggc gcagatactc ttccaccacg cgcagccgcg cttcctcacc
     4981 tgcagccgag cacacctcgt tgagtaagcg gtcggcttcg gacggataaa gctcctgcag
     5041 ggggatatcg atgttgtgca gctcgtgcgc tgccacgggg aaaaacatgc gagcggctcc
     5101 gggacgaaaa acaacgccca ccacccccgt cgaaagcgaa ggatcgatct gaaacgactg
     5161 agaccaaggg ccggccagca agggaacgcc gatactgcgg gtttcctcac ccacgaaata
     5221 ggtcgccgtc ggcttttcca ggtggatcac caaagccatt gcgccgtctg gatacacccg
     5281 ctgccgagac ggggccatcc cggaagtgga tgcgtaccac agcgattcga cataacggga
     5341 gagcgggagg gccggtttgt gaatccggaa catgccgtca tcctacaaac gtcgacgggc
     5401 ccgtcgcccc ttcttgtgac caatcggccc ggttgggtgt ccgtcctgca gcctcctggc
     5461 ccgctccgcc cgtccagcct gccatcccta tataaggatg agataataaa agcagctgtc
     5521 aggagatcct ctcctcggcc gccgtccaat gggggcgtca cggtttcgac gggatcgatt
     5581 gcggcaaaga ggcatgccgg ggtctgggca cccgtaatcg cctggaaaac cataattgcc
     5641 aacaccaatc agctggcata cgcagcctaa ttagttagac tgccgttctc ctcggcttcg
     5701 ccctcgggct gagacaggac gtcgcactgg gggctggctt ccgatcctcg gtccgtggat
     5761 cggcggcgag atcatcggac tagttttcgg cgtttcttgt ccgttctaag cgccgggagc
     5821 gaaacttcgg aacggactga gcatggagtc tctcggctga caacgatttc ggacgcgggt
     5881 tcgactcccg ccgcctccac caaccacctt ccattacgtt tctttgacaa tcgcctgccc
     5941 gctcgacccg gtgcatagca tccaatccag ggcaggttcc ctaactgtca gcgatcctca
     6001 gaaaaaggaa gaaaacctat atgcctaaaa tcacaacgcg ggtgaaagac ctgccgggat
     6061 ggatgccgag gcctatcgga ctaccatcgc gatttgcccc cagcgacagc gatgcgttgg
     6121 gtgaagcaac cgtcgaacac atcctccgtg tcatggacga ccgcatcgta ttcacctgca
     6181 agtacaaggg gcgacggatc gattttgact tcagcactga ggacagaaag accagcgaca
     6241 aggttgcgga aatcctgaga agccatcggg agaaaagcct tgcctcgatc ggcaccatcg
     6301 agcttcctgc ggatctatta gctgcgtagg aaa
//
LOCUS       DASZYN010000306         2390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_203633, whole genome shotgun sequence.
ACCESSION   DASZYN010000306 DASZYN010000000
VERSION     DASZYN010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203633"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1616
                     /locus_tag="VHE33_07980"
     CDS             270..1616
                     /locus_tag="VHE33_07980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT97431.1"
                     /translation="MPEPIAAEMQQVSQKPVYDLRKQQTLFSVLTLFVLAMLLLLHML
                     FAVVLGEPSVSVIVVLGLSFALRLAELGWLQGRSEPLSDFAAKLDGLVSIVSLFVLAV
                     LLAWLTDRDHSPYQVLLIIPVLQAATLFRLTPTLLTILAADATIFLWLWHYFALYPPA
                     LLDEYLEAGMFAVVVALVGVLMWVLMRILRSHQTALDRTLSDLQSARESLVREEKLAA
                     VGRLASGIAHEIRNPVAMITSALATAEDGAADAKERAEMFAIAHHQAKRLEKLTTGFL
                     NYARPAVPRRVSIAVDDLIGAVEAVTRIRAEERGIRVSSRIERHGTASIDASQVEGAL
                     LNLCLNAVEAMDSNGEIRLLADLEGDVLRIEVRNSGHAIPQPYLERIFEPFFTTRANG
                     TGLGLAIARGIARAHGGDLWVSSNEEGSVAFTMTLTSGTPEQTIQEEIRGQDPHRR"
     gene            1591..>2390
                     /locus_tag="VHE33_07985"
     CDS             1591..>2390
                     /locus_tag="VHE33_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HVT97432.1"
                     /translation="MGKILIVDDEASMRRILVANLRRDSHVVVEASGFGEARALLARE
                     DFDAVLTDQKMPDGSGLDVLRAVQDDDATTSVIFLTAVGTVELAVESMREGASDFLTK
                     PFVPEAVRTAVARACERTALLRENAVLRSTVRKLEDPGDILGASAAMEAVRDVIARVA
                     GTNSTVLIMGETGTGKELVARAIHRNSRRAASAFIAINCAAVSETLLESELFGHEKGA
                     FTGADKARAGLFEAAHNGTLFLDEVAEMSPAAQAKLLRVLANGEIQRVG"
BASE COUNT          425 a          799 c          706 g          460 t
ORIGIN      
        1 gccgtgttcc tgatgcagac caccgattcg gcagccgtgg tgacgcagac cctcgggatt
       61 ggcgccgacg atagcggtgc ctctcatcgc gccgaggcgc cggtctcgca acgaaagtga
      121 tacgcaattt cggtgtcctt ccttcctcag ggcgtaacct gatcacaaat cgcctgatgc
      181 gccggaatgc tcccatcgtt cgtatcccgg ctcattccag ggattgcgag caaggcgaag
      241 cagtgccgtc atgccggcgg atccaaccga tgcccgaacc catcgcagcc gaaatgcaac
      301 aggtgagcca aaagccggtt tacgatctgc gcaagcagca aaccctcttt tctgttctga
      361 ccctttttgt gcttgccatg ctgctgctcc tgcacatgct cttcgcggtc gtgctcgggg
      421 agccgagcgt ctccgtcatt gttgttcttg gactgagttt tgctctgcgt ctggccgaac
      481 tcggctggtt gcagggccgc agcgaacctc tcagcgactt tgccgcaaag ctcgacggcc
      541 tcgtttccat cgtcagcctc ttcgtgttgg cggtactgct ggcctggctg accgatcgcg
      601 atcacagccc ctaccaggtt ctgctcatca ttcccgtcct ccaggcagca accctgttcc
      661 gcctgacgcc cacactgctg accatccttg ccgccgacgc caccatcttc ctctggctgt
      721 ggcactattt cgcgctctac ccgcctgcgc tgctcgacga atatctcgag gccggcatgt
      781 tcgccgtagt ggtggctctg gtcggcgtcc tcatgtgggt gctcatgcgc atcctgcgtt
      841 ctcatcagac tgccctggac cgtaccttgt ccgacctgca aagcgcccga gagagcctgg
      901 tcagggaaga gaagctcgct gccgtgggcc ggctcgccag cggtatcgcc catgagatcc
      961 gcaaccctgt cgccatgatc accagcgccc ttgctaccgc ggaagacggt gccgcggatg
     1021 cgaaggagcg cgcagaaatg ttcgccatcg cccaccacca ggcaaagcgt ctcgaaaaac
     1081 taaccaccgg gttcctcaac tacgccagac ccgccgtgcc gcgtcgcgtc tcgattgcgg
     1141 tggatgatct gatcggcgcg gtcgaagccg ttacgcgtat tcgcgccgag gagcgcggca
     1201 tccgggtttc ctctcgcatc gaacgtcacg gcaccgccag cattgatgcc tcccaggtcg
     1261 agggcgctct tctgaatctg tgcctcaacg ccgtcgaggc catggacagc aacggcgaaa
     1321 ttcgcctgct cgccgatctg gaaggtgacg tgctgcgcat cgaggttcgg aattccggcc
     1381 atgccattcc tcagccatac ctcgagcgca tttttgagcc ctttttcacc acccgagcca
     1441 acggcaccgg tctgggcctt gccattgcaa gaggcatcgc cagggctcat ggtggagatc
     1501 tgtgggtgag cagcaatgaa gagggaagtg tcgccttcac catgacgctg acctccggca
     1561 caccggaaca aaccattcag gaggaaatcc gtgggcaaga tcctcatcgt cgatgatgaa
     1621 gcgagcatgc gaaggatcct cgtcgccaac ttgcggcgag acagccacgt tgtcgtcgag
     1681 gcctccgggt tcggcgaagc ccgcgcgctg ctggctcgcg aagatttcga cgcggtcctg
     1741 accgaccaga agatgcccga cgggagcggc ctcgatgtgc tgcgcgccgt ccaggacgac
     1801 gacgccacca catcggtgat cttcctgacc gcggtgggca cggtcgagct cgccgtcgaa
     1861 agtatgcgcg agggcgcctc cgactttctc accaaaccct ttgtcccgga agccgtgcga
     1921 accgcggtcg cccgtgcctg cgagcggacc gccctgctgc gcgagaatgc cgtgctgcgc
     1981 tccacggtgc gcaagctgga ggacccaggc gatatcctcg gcgccagtgc ggccatggag
     2041 gccgtgcgcg acgtgatcgc gcgcgtggcc ggtaccaact ccaccgtgct catcatgggt
     2101 gaaaccggca ccggcaagga gctggtcgcc cgcgccatcc atcgcaacag caggcgcgcc
     2161 gccagtgcgt tcatcgccat caactgtgcc gcggtttctg aaacgctttt ggaaagcgaa
     2221 ctgttcggcc acgagaaagg cgcctttacc ggagccgaca aggccagagc cggactcttc
     2281 gaggcggcgc ataacggcac cctcttcctc gacgaggttg ccgagatgtc gcccgccgcc
     2341 caggccaagc tgctgcgcgt gctcgccaac ggtgaaattc agcgcgtcgg
//
LOCUS       DASZYN010000307         8307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_780358, whole genome shotgun sequence.
ACCESSION   DASZYN010000307 DASZYN010000000
VERSION     DASZYN010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8307
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780358"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VHE33_07990"
     CDS             complement(<1..732)
                     /locus_tag="VHE33_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HVT97433.1"
                     /translation="MATIVESPKPFTMADFKGRVDPDWCPGCGDFGVLAAVHKALVDL
                     QIPNHMICTISGIGCSSNFPGFIETYGMHTLHGRSLPVATGVKMANPDLTVLVTGGDG
                     DGLGIGGNHLTHTIRRNVDLTYIIMDNQIYGLTTGQTSPTSRIGMKTKSTPFGSIEGP
                     LYPITMALAAGATFVGRGFSGDQKHLTELIKQAIQHKGFSFLDVFSPCVTYNKDNTFQ
                     WFRPRVKRLEDDSTYDPTNWIGAMEK"
     gene            complement(999..2774)
                     /locus_tag="VHE33_07995"
     CDS             complement(999..2774)
                     /locus_tag="VHE33_07995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HVT97434.1"
                     /translation="MTQTFSIGIGGAAGQGVATPGDIFAKIFSRRGLHLNAYNAYQSI
                     IRGGHTFLTIRAGVDPVTNMGDTLELLIPLNQDTMDRHLRLLTAGGACIYNADSIKPG
                     EHADGVQLCPLPVSTLADITKNKVAQNTLAMGAALSMMAIGFESLESVIREQFGKKAA
                     AVADENIAVARAGYDYATQNFKPFANPVPMTDTKYAVLSGNAALAMGGAAAGVKFYCA
                     YPMSPSTGVLHWFAANGRKAGIMVRQVEDEIGVVNMVIGAAQAGVRAMCATSGGGFAL
                     MSEALGLSAMAEIPIVVIDCMRAGPSTGVPTKTEQGDLWQMLGAAFGDYPRVIAAPLD
                     IGDCFTLMAEIWNICDKFQVPGLVLADLLLSEGRLSVDPKILDFKPHIDRGEMITEPN
                     GDHGEYLRYRITESGVSPRAIPGVPGYTHVVSSDEHDENGVLISDMYTNAGKRRAMME
                     KRMRKEQGIAAAVPPPRLQGPKDADVTLIGWGSTQGVIEEARAQLAEQGITANQLQIR
                     WLVPLHGEAILDQLRNAKRTIIVENNFSGQFARYLRSETSFVPDGHIRKYDGEPFMPH
                     HIVEAVREQLAGDTTVSVPTHEIMV"
     gene            complement(2868..4136)
                     /locus_tag="VHE33_08000"
     CDS             complement(2868..4136)
                     /locus_tag="VHE33_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97435.1"
                     /translation="MSPSDAYLYAMQPLNDARKAPDDLTDADKWALAIGVARAKEQCE
                     LRSKDKFDGEDLLAMGKLCIFGQDYEPARAFLVSYNALPQPQSPEVGHLLLARAFIGL
                     GAVPSAESEIQTLLSLFPYDASIHLGIDMVIDAASASDATEDLDLIPRLEAQQLPHIF
                     DAFAHGGSLSGNGDSVDAALLTRDALRCADALRRNFKPEDAAKILDQLKAALAAPSIV
                     ASASYPSIQAAMARYDLFRRSSPVREFHGAELTATGSSIPRAVPVYDTNPAAHRIVRH
                     IDAKTTTIRILDDRTLVLLFSLGSPACSETIHGILDLLARDHVTPGLKVIAATSFAAN
                     TGVDAPTPQLLAALRAFRAGLPPELPVLILPDAQLKPFSVDSWPAAILFDGRGRIRWL
                     NTISGSSGSIHQTVRELEMPVPVDFPSNSN"
     gene            complement(4378..5115)
                     /locus_tag="VHE33_08005"
     CDS             complement(4378..5115)
                     /locus_tag="VHE33_08005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HVT97436.1"
                     /translation="MKTRLDKLLVERALVPTRERAQAVILAGRVLVNEQKIDKPGHAV
                     PDDAPVRLLGSDLRYVSRGGLKLEAALDHWHIDLTGQFCCDIGASTGGFTDCMLQHGA
                     ARVLAIDTGYGQIAQTLRDDPRVTLMERTNARLLNPGDLPPGISFLAVDVSFISATLV
                     LPPVLAALAESPAVVAVLLVKPQFEAGRERIGKGGIVRDPAAHQFAIDRVRACVEELG
                     GQQIDVIDSPITGAEGNREFLLYSIWP"
     gene            5229..5774
                     /locus_tag="VHE33_08010"
     CDS             5229..5774
                     /locus_tag="VHE33_08010"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HVT97437.1"
                     /translation="MTDETTQIRQRYPLTWLGWLLAAVFAVIAAVLAHHVAWLRGELN
                     TSQGSAAQMKVQLDHANQIVAVLSSPQSAHVMLTETRQAMRPVGQVSWLQSQGALVFV
                     AGGLRDLPSDKAYELWLIPEGGKAPIPAGLFRPDPDHGATVVLPPVPADTQARKFEVT
                     EEPGHGSETPSLPIVMQGESR"
     gene            5879..6586
                     /locus_tag="VHE33_08015"
     CDS             5879..6586
                     /locus_tag="VHE33_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HVT97438.1"
                     /translation="MGEKSLVTLAVDIGGTGLKMLCLDAEGKPITERVRVPTPDPPTP
                     TRLLTALDELKAQMPDFDRVSVGFPGVVKGGMTMTAVNLDPRWVGYPLEANLKKRWKK
                     PVRVANDAAVQGLGAIQGRGIEMILTLGTGMGSCLFVDGHICPLELGHHPWLKKTYED
                     YLGRRGLKKYGVKKWNKLLRKAIAQTHATFNWDHLYLGGGNTKLMTLQVRPNVTIVSN
                     IDGLLGGVALWRDEGSQ"
     gene            complement(7042..>8307)
                     /locus_tag="VHE33_08020"
     CDS             complement(7042..>8307)
                     /locus_tag="VHE33_08020"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97439.1"
                     /translation="ESIDLAEDRKRFGKLLEELEIPQPPGVIATSVEEALRGAERIGY
                     PVLVRPSYVLGGRAMVIAFDAESVSRYMKEAVEYSQERPILVDHFLEDAVEVDVDALC
                     DGEDVVIAGIMQHIEEAGIHSGDSSCVLPAVDLVPATLDTIRGYTRRLALALNVIGLV
                     NLQFAIQRKPGMADKVFVIEVNPRASRTVPYVSKATGVPLAKIASRLMTGRRLREFLD
                     VSSHKDLETGSHFYVKSPVFPWSKFQGVDTVLGPEMKSTGEVMGVADNFGEAFAKAQL
                     SAGLQLPLKGTVFFSVSDRDKTNLVNLARQYMELGFHLVATEGTANAIERAGMTVERV
                     YKVKEGRPNVVDLIKGERIQLIINTPRGADPYFDEKAIRRAAVIARIPTITTMAAARA
                     AVEGIAALQRRQITVNPLQKLHAEHLAVR"
BASE COUNT         1569 a         2184 c         2995 g         1559 t
ORIGIN      
        1 tttctccatc gcgccgatcc agttggtcgg gtcgtaggtt gaatcgtctt cgagccgctt
       61 cacgcgcggg cggaaccact ggaaggtgtt gtccttgttg taggtgacgc aggggctgaa
      121 gacgtcgagg aaggaaaagc ctttgtgctg gatggcctgc ttgatcagtt cggtcaggtg
      181 cttctggtcg ccggagaagc cgcgccccac gaaggtggca cccgcggcca gcgccatggt
      241 gatgggatag agtgggcctt cgatgctgcc gaagggggtg ctcttggtct tcatgccgat
      301 gcggctggtg ggcgatgtct gcccggtggt gaggccgtag atctggttgt ccatgatgat
      361 gtaagtcaga tcgacgttgc ggcggatggt gtgcgtgagg tgattgccgc cgatgccgag
      421 gccgtcgcca tcgccgccgg tgacgagcac ggtgaggtcg ggattggcca tcttcacgcc
      481 cgtcgccacc ggcagggagc ggccgtggag ggtgtgcatg ccgtaggttt cgatgaagcc
      541 aggaaagttc gacgagcagc cgatgccgct gatggtgcag atcatgtggt tgggaatctg
      601 gaggtcgacg agggccttat ggacggcggc aagaacgccg aagtcgccgc agcccgggca
      661 ccagtcagga tcgacgcggc ctttgaaatc ggccatggta aacggcttgg gggattcaac
      721 aatggtggcc attttcttct cctggaactt ccctcagggg ctaaagcccc ggttcttctg
      781 cggacctttc ggcacgagtc aactcgtgcc ctgatacaaa aacgccgggg ctgaagcccc
      841 cgatctctct ctgcccttca ttccccggcc tgaaggccgg ggcttctacc gtcgtgccgc
      901 ttcgcgacac aggatgcatt tccttttcca aaccttctgc cgtcgtgcct gcttcgcggt
      961 gcaggatgaa cctcgaattc ggaccactcg attgattcct agaccatgat ctcgtgcgtt
     1021 gggacggaca cagtggtgtc gcccgcaagt tgttcgcgta ccgcttccac aatgtggtgc
     1081 ggcatgaagg gctcgccatc gtacttgcgg atgtggccgt cggggacgaa gctggtttcg
     1141 ctgcgcaggt agcgggcaaa ctggccgctg aagttgttct cgacgatgat ggtgcgcttc
     1201 gcgttgcgca gctgatcgag aatcgcttcg ccgtggaggg gaacgagcca gcgaatctgt
     1261 aactggttgg cagtgattcc ctgctcggcg agctgggcgc gggcttcttc gatcacgccc
     1321 tgcgtggatc cccatccgat gagcgtaacg tcggcgtctt tcggaccctg gaggcggggg
     1381 ggcggcacgg cggccgcgat tccctgctcc ttgcgcatgc gcttttccat catggcgcgg
     1441 cgcttgccgg cgttggtgta catgtcgctg atgagcacgc cgttctcatc gtgctcgtcg
     1501 ctggagacga cgtgcgtata gccagggacg ccggggatgg cgcggggcga gactccgctt
     1561 tcggtgatgc ggtagcgaag atattcgccg tgatcgccgt tgggctcggt gatcatctca
     1621 ccgcgatcga tgtgcggctt gaagtcgaga atcttcgggt cgacgctgag gcggccctcg
     1681 gagagcagca ggtcagcgag gacgaggccg ggcacctgga atttgtcgca gatattccag
     1741 atttcggcca tgagggtgaa acagtcgccg atgtcgagcg gcgcggcgat cacacgggga
     1801 tagtcgccga aggcagcgcc gagcatctgc cacaggtcgc cctgttcggt tttggtggga
     1861 acgccggtgg acgggcccgc gcgcatgcag tcgatcacaa cgatgggaat ttccgccatg
     1921 gcagagaggc cgagggcttc ggacatgagg gcaaatccac cgccggaagt ggcacacatg
     1981 gcgcgaacac cggcctgggc ggcgccgatg accatattga ccacgccgat ttcatcttcg
     2041 acctggcgga ccatgatgcc ggctttgcga ccgttggcag cgaaccagtg gagcacgccg
     2101 gtggagggac tcatgggata ggcgcagtag aatttgacac cggccgcggc tccgcccatg
     2161 gccagggcgg cattgccgct gaggacggcg tacttcgtat ccgtcatggg gacgggattc
     2221 gcgaagggct tgaagttctg cgtggcgtag tcgtatccag cgcgggcgac ggcgatattt
     2281 tcgtcggcaa cggcagcggc cttcttgccg aactgctcgc ggatcacgga ttcgagagac
     2341 tcgaaaccga tggccatcat gctgagggcg gcgcccatgg cgagggtatt ctgcgcgacc
     2401 ttgttcttgg tgatgtcggc cagagtggag acggggagcg gacagagctg cacaccgtcg
     2461 gcatgttcgc ccggtttgat ggagtcggcg ttgtagatgc acgcgccgcc ggcggtgagc
     2521 aggcggaggt ggcgatccat ggtgtcctga ttgagcggga tgagcagttc gagggtgtcg
     2581 cccatgttgg tgacgggatc gacgccggcg cggatggtca ggaaggtatg gccgccgcgg
     2641 atgatggact ggtacgcgtt ataggcgttg agatggaggc cgcggcggct aaagatcttc
     2701 gcaaagatgt cgcctggagt ggcgacaccc tggccggcgg cgcctccgat gccgatggaa
     2761 aaggtctggg tcataaggct cccgcaaaaa ctggcagagt ccaggcgtag gttcctgaag
     2821 tccgaccagc atacaacagc gcatggacga ttcgacaggg gcggaggtca gttggaattc
     2881 gatggaaaat cgacgggcac gggcatttcg agctcgcgca ccgtttgatg aatgctgccg
     2941 ctggaaccgg agatcgtgtt gagccaccgg atgcggcccc tgccatcgaa caggatcgcg
     3001 gcgggccagc tatcgacaga gaagggcttg agttgggcat cggggaggat gagcacgggc
     3061 aattccggcg gcagaccggc gcgaaaagcg cggagcgcag caaggagctg aggggtgggc
     3121 gcgtcgactc cggtattggc ggcgaaagag gtggcggcga tgactttgag gcccggcgtt
     3181 acgtgatcgc gggcaagaag gtccaaaatg ccgtggatgg tctcgctgca ggcgggactg
     3241 ccgagggaga agagcaggac aagcgtgcgg tcgtcgagga tacggatggt ggtggttttt
     3301 gcgtcgatgt gccggacaat gcgatgcgcg gcggggttgg tgtcgtaaac gggaacggca
     3361 cgggggattg aagatccggt ggcggtgagt tcggcgccat ggaattcgcg cacggggctt
     3421 gaccgccgga agagatcgta gcgagccatg gcggcctgga tggacgggta actggcgctg
     3481 gcgacgatgg agggcgcggc gagggcagct ttgagctggt cgagaatttt cgctgcgtct
     3541 tccggtttga agttgcggcg cagggcgtcg gcacagcgca gggcatcgcg cgtgagcagc
     3601 gcagcgtcga cagagtcgcc attgccgctg agcgagccgc cgtgcgcaaa ggcgtcaaag
     3661 atgtggggca gttgctgcgc ttcgaggcgg gggatcaggt cgaggtcctc ggtggcgtcg
     3721 ctggcggagg cggcgtcgat gaccatgtcg atgccgagat ggatgctggc gtcgtacgga
     3781 aagaggctga gaagggtttg tatctcgctt tcggcagagg ggacagcgcc aagaccgatg
     3841 aaggcacgag cgagcagcag atggccgact tcaggagact ggggttgcgg cagcgcgttg
     3901 taggaaacca ggaacgcgcg agcgggctcg tagtcctggc cgaagatgca gagcttgccc
     3961 atagcaagaa ggtcttcgcc gtcgaatttg tctttgctgc gaagctcgca ttgctctttc
     4021 gcgcgggcaa cgccgatggc gagcgcccat ttgtcggcat cggtaaggtc gtctggagcc
     4081 ttgcgggcat cgttgagagg ctgcatcgcg tacaggtatg catcggatgg gctcatggga
     4141 ggggcgccgg cgctggggtt agcggaagcg gatggggttt gggcgggcag attgggggtg
     4201 gcggaaggtg gcggcgcctg cgatggagga ctggcgatgg caggctgaga tgtgggagcg
     4261 ggcggggctt gttgtccaaa aagagttgag ctcaggagtg ccaggagcag cagccgcatg
     4321 cgtcgcaggg taccaccgtc ggaggcggga cgccggatcg ggtttgggct gaggaggtca
     4381 cggccagatt gagtagagaa ggaattcgcg attgccttcg gcgccggtga tgggggaatc
     4441 gatcacgtcg atctgttggc caccgagttc ttcgacgcag gcgcggacgc gatcgatggc
     4501 gaactggtgg gcagccgggt cgcgaacgat gccgcccttg ccgatgcgct cgcggccggc
     4561 ttcaaactgg ggtttgacga ggagtactgc gactacggcg ggactttctg cgagcgctgc
     4621 caggaccggc ggcagaacga gggtggcgga gatgaaggac acgtccacag caaggaagga
     4681 gatgccgggc ggcaggtcgc cgggattgag cagacgggcg ttggtgcgtt ccatcagggt
     4741 cacgcgcggg tcgtcgcgca gggtttgagc gatctggcca tagccggtat cgatggcgag
     4801 aacgcgggca gcgccgtgct gcagcatgca gtcggtgaag ccaccggtgg aggcgccgat
     4861 gtcgcagcaa aattgcccgg tgaggtcgat atgccaatgg tcgagagccg cttccagctt
     4921 gagtccgccg cggctgacgt agcgcagatc ggagccgagg aggcgaaccg gcgcgtcgtc
     4981 gggcacagcg tggccaggct tgtcgatttt ctgctcgttg accagaaccc ggccggcgag
     5041 gatgacggcc tgtgcccgct cgcgggtggg aacgagagcg cgttcgacga gaagcttgtc
     5101 cagacgcgtt ttcattgtct gctcttgacc gccggcgcgc gggtggccgg agccgacgcg
     5161 acacgtgggc tgccgatgtc gcatcctaag agagaaagtg agcggggcat gtacccgaat
     5221 attgtgcgat gaccgacgaa acgacacaaa tcaggcagag gtatccgctg acatggctgg
     5281 ggtggttgct ggcagcggtg tttgcggtaa tagcggcggt actggcgcac catgtggcgt
     5341 ggctgcgggg cgaactgaac acatcgcagg gcagcgcggc gcagatgaaa gtgcagctgg
     5401 accatgcgaa ccagatagtg gcggtgctga gttcgccgca gtcggcgcat gtcatgctga
     5461 cggagacgcg gcaggcgatg cggccggtgg gacaagtgag ctggctgcag agccagggag
     5521 cgctggtctt cgtggcgggc gggttgcgag acctgccgtc ggataaggcg tatgagctgt
     5581 ggctgatccc tgagggcggc aaggcgccga ttccggcggg actgtttcgg ccggatccgg
     5641 atcatggagc caccgtggtg ctgccgccag tgcctgccga tacgcaggca agaaaatttg
     5701 aagtgacgga ggaacctgga cacgggtcgg agacgccgtc gctgcctatc gtgatgcagg
     5761 gagaaagccg atagaaccag tagtcatcgg cacggcgggc cgccgaacgc cgacgaagag
     5821 ttttgccatc gcgcacaatc aggagcgcga tacactgacc cgggtttgtt ctggagttat
     5881 gggcgagaaa tcactggtca cgctggcggt ggatatcggg ggcacggggc tgaagatgct
     5941 gtgcctcgat gcggagggga agccaatcac agagcgggtg cgggtgccga cgccggatcc
     6001 gccaacaccg acaagactgc tgacagcgct ggacgagttg aaggcgcaaa tgccggattt
     6061 cgatcgggtg agtgtggggt ttccgggcgt agtcaaaggc ggcatgacga tgaccgcggt
     6121 gaatctcgat cccaggtggg tgggatatcc gctggaagca aatctgaaga agcgctggaa
     6181 gaagccggtg cgcgtggcca acgacgccgc ggtgcagggg ctgggtgcga ttcagggacg
     6241 gggcatcgaa atgatcctga cgctgggaac gggcatggga tcgtgcttgt ttgtcgacgg
     6301 acatatctgc ccgctggaac tggggcatca tccgtggctc aaaaagacct atgaggacta
     6361 tctgggccgg cgcggtctga agaagtacgg cgtgaagaag tggaacaagc tgctgcgcaa
     6421 agccatcgcg cagacgcacg cgacgttcaa ctgggatcat ctttatctgg gcggtggaaa
     6481 cacgaaactg atgacgctgc aggtgcgccc gaacgtaacg attgtgtcga acatcgacgg
     6541 gctgctgggc ggcgtggcgc tctggcggga tgaaggtagc cagtagccag tagctttgga
     6601 cgccgcgatg gctatgatct aaagagcctg cgcaggaact ttctgacaaa tccagagctc
     6661 atggcttctg cggcagagaa gctgggtgga gtgccccatt tgctttcgcg aaaagtcgta
     6721 ccggacacag gatgatcaca attaccggtt ccgacacgag cctgtatatc cggatctaac
     6781 ccagcgtagc gatggcagcc aagctcctga ccgtcaaaga ccgcgaacgc ctgtctcagt
     6841 tcagattcag tcactggctg cttcttccag tagcattgcc tcatctcgtc gctggtccag
     6901 cccacaaggt caggcgctac ggcttgcgga actccacacg aaagacagca tcgggcatcg
     6961 acgtaaaaac catctgcctg accagtagct cggggagggg acgagtcatt cttcacagag
     7021 agtcctgccc gtagttttat ctcagcgtac cgctagatgt tccgcgtgca gcttctgcag
     7081 cggattcacc gtgatctgcc ggcgttgcaa tgcggcgatg ccctcaactg cggcacgtgc
     7141 ggcggccatg gtcgtgatgg tgggaatgcg cgcgatgaca gcagcgcggc gaatggcctt
     7201 ctcgtcgaag taaggatcgg caccacgcgg cgtgttgatg atgagctgga tgcgctcgcc
     7261 tttgatcagg tcaacgacgt tggggcggcc ttccttgact ttgtagacgc gctcgacggt
     7321 catgccggcg cgctcaatgg cgttggcggt gccctcggtg gcgacgagat ggaagccgag
     7381 ttccatgtac tgacgcgcca ggttgacgag gttggtcttg tcgcggtcac tgacgctgaa
     7441 gaagaccgtt cctttgagcg gcagttgcag accggcagag agctgtgctt tggcgaaggc
     7501 ttcgccgaag ttgtcggcca cgcccatgac ctcgccggtg gatttcatct ccggccccag
     7561 gactgtgtcg acgccctgaa acttgctcca cggaaagacg ggcgatttta cgtagaagtg
     7621 cgaaccggtc tcgagatcct tgtgcgagga gacgtcgagg aattcgcgca ggcggcggcc
     7681 agtcatgagc cgtgaagcga tttttgcgag cggcacgccg gtggccttcg agacgtacgg
     7741 aacggtgcgc gaggcgcgcg gattgacttc gatgacgaac accttgtcgg ccataccggg
     7801 cttgcgctgg atagcgaact gcagattgac gagtccgatg acattcagcg cgagcgcgag
     7861 acggcgcgta tagccgcgga tcgtgtccag cgtggcgggg accaggtcga cggcgggcag
     7921 aacgcacgac gagtcgccgg agtggatacc ggcttcttcg atgtgctgca tgatgccggc
     7981 gatgacaacg tcttcaccat cgcagagcgc atcgacgtcc acttcaacgg cgtcctcgag
     8041 aaagtgatcg acgaggatgg ggcgctcctg agaatactcg acagcttcct tcatgtagcg
     8101 agaaacgctt tcagcgtcga acgcgatgac catagcccgg ccgccgagca catacgaagg
     8161 tcgaacgagg acgggatagc cgatgcgctc ggcgccgcga agagcttctt cgacgctggt
     8221 ggcaatcacg ccgggcggct gcggaatttc taattcttcg agtagctttc cgaagcgctt
     8281 gcggtcctca gccagatcaa tcgactc
//
LOCUS       DASZYN010000308         4916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1106307, whole genome shotgun sequence.
ACCESSION   DASZYN010000308 DASZYN010000000
VERSION     DASZYN010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106307"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(705..1091)
                     /locus_tag="VHE33_08025"
     CDS             complement(705..1091)
                     /locus_tag="VHE33_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HVT97440.1"
                     /translation="MSGHPAGIDYSALRVAVPMEQVLRLLGYRHASRRGQQLRGPCPV
                     HDPSAAGGSRCFSAHLGRHVFRCFHCGAQGNQLDLWQLVHELPLYEAALLLCREANIE
                     PPPAMLVTPRNPKICNSPSPPSRMAT"
     gene            complement(1100..1900)
                     /gene="istB"
                     /locus_tag="VHE33_08030"
     CDS             complement(1100..1900)
                     /gene="istB"
                     /locus_tag="VHE33_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HVT97441.1"
                     /translation="MKKENPALLRQALLENFRTLKIPLTDQQLDAALARAEQEGLSHL
                     QFVHCLIADQASGRRERSIERRIQEARFPGLGTLESFDWKFNEKTIDRGRFEELAAGE
                     FVRRGDNLVMVGQSGLGKSHLIEAVGRRLCSIEYRVRYVTSADLVKDLATSRADDTLP
                     RRLRYYGNFDLLIIDEFGFEKIERIDHPQAANLLYKIIEARYPRRSTALVTNVDFETW
                     DEYLGDPPLAMAFLDRVVDGAIVLKLEGKSYRAHRARRIDAKPKPSDE"
     gene            complement(1812..3467)
                     /gene="istA"
                     /locus_tag="VHE33_08035"
     CDS             complement(1812..3467)
                     /gene="istA"
                     /locus_tag="VHE33_08035"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HVT97442.1"
                     /translation="MNQELTHEIVRRYHQGQSTRGIARDLQISRERVRRTLAGHHQAR
                     NGEVPASSLPPPKQKRKSLLDDHEDVMRQLLGRYPRMTAVRMLEELRARGFQGGYSIL
                     KERMAQLRPQPAQPLVERFETGPGLQAQMDYAQYDLDFTLEGRRRVYLFSYLLAYSRR
                     QYLCFTESQDFATTVRQHVKAFEHLAGVAAVCLYDNMKVVVSRYEADLPVYNTRFLAF
                     ATHYGYRPKACRPRHPATKGKVERPFHYVEMNLLNGREFRSLDHLNEVTRRWLAEVAD
                     VRLHRQTQRRPVDLHAEEQPHLIPLPNHPYDAAEVVYRSVDCEGYVKYRQNQYSVPWR
                     YVGQVLPVRITEEELIVFSSQIEEIARHRLLPRSASRQCQLDSAHRPPDDRRQQYELL
                     RGSFAEFGEVGERFFEGLLKAHRQGKAQARNILALRAHYHDRDLIKALERALRYGAFS
                     FYAVERILHVQAQPKTALEMLADREKDHLADLLRDASVPPRPTGDYQQLLSNEESHAQ
                     EEADPAKADPANPGDEEREPGPPPSGPPGELPNSEDSPDGPAA"
     gene            complement(3464..4381)
                     /locus_tag="VHE33_08040"
     CDS             complement(3464..4381)
                     /locus_tag="VHE33_08040"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HVT97443.1"
                     /translation="MASHQWGSTDCLPLAEIDLDFHRYRLVHRQAEETMVQSLRRYGQ
                     LSPIVVCATEGRLLLLDGFKRHAAAGRVKGMDTLWSRRLEADAAAAKAAVYTLNSLAR
                     PVQALEEAWIVHALIREDGLSQPAVAELLGRHKSWVCRRLALLERLAPEVREDLGLGL
                     VSPTAARQLTRLPAGNQLKALETVRRESLTAAELRGVVDLLLASGTQEKQRYVLEKPR
                     QALREFEGHVSRSWDPRMSAAGNHLSRRLAKLLDLLAGLESWLRYSGRGVLELRDREP
                     LRPGLERLLLDCRGVAELTEDFLRELHLP"
BASE COUNT          912 a         1533 c         1551 g          920 t
ORIGIN      
        1 aaacttcggt cagcgagaca ttcgtttggt cggcgatcga agacgcagcg gtttccatgg
       61 ctttacagtc gaagcgtgtg cgatgcgggt gaatcgggca aatcgacgac actctctttt
      121 gttgcagtcc gttggggatt caccggagga tgttggcctt gtcataaggg aggcaaacgg
      181 cgtaccgtcg cgcacagcaa cccagtcaac gtatgcatgc gccttccagc tcgtccaacc
      241 actccagcac tttcgccaca aactcagccg gcaagcactc gtcgcacgct tgcagaattg
      301 tagggcaggc caggtgttcg gcaacgaagt caagatacag gccccggaga tcgactgggc
      361 tgccgctctg cacgtgccat ctggccaaga cctcgccatt gatcgtgatt ctgcccccgc
      421 atgtcccgtc ctcgttgcgc tgaccctgga attcagctcg cattactccg cgcatggctg
      481 ctcccttggc tgcaaagtat gtcgagtcgc gcgcaatgcg cgatcatcgg tgacgaatgc
      541 cgtgcggcgt gcccggtgtt gtgaacggcg gtcgaaaagt gcagcgcgcg acggttgaaa
      601 agtgcagcgc tcggaagggt aaggtctagt ttacgtaacg tctcctgagg agtcggtgcc
      661 ggttaaaaac gaggccagtg tggcggccga tggcttacca gtggtcaggt tgccatgcga
      721 gatggtggcg agggagagtt gcagatcttg gggtttcgcg gtgtgaccag cattgcaggc
      781 ggcggttcga tgttagcctc gcgacagagc agaagggcgg cttcgtagag cggcagttcg
      841 tggacgagct gccataaatc gagctggttt ccctgggcgc cgcaatggaa gcagcggaag
      901 acgtgacgtc cgagatgggc ggagaagcag cgtgacccgc cggcagccga gggatcgtgg
      961 acggggcacg gaccgcggag ttgctggccg cggcgagaag cgtggcgata gccgagcagc
     1021 cggagcactt gctccatggg gacggccact cgcagcgcag agtaatcaat gccggccggg
     1081 tgcccagaca tgccggcgtt tattcgtcgg acggttttgg tttggcatcg atgcggcgag
     1141 cccgatgggc ccgataggac ttgccttcca gcttcaacac gatggcgcca tcgaccacac
     1201 ggtccaggaa ggccatggcc aggggcgggt cgcccaagta ttcatcccag gtctcgaagt
     1261 ccacgttggt caccagcgcc gtgctgcggc ggggatagcg ggcctcgatg atcttgtaca
     1321 gcaagttcgc cgcctgggga tggtcgatgc gttcaatctt ctcgaagccg aactcgtcaa
     1381 tgatcaacag gtcgaagttg ccgtaataac gtagccgccg tgggagcgtg tcgtccgccc
     1441 gggaggtcgc caggtccttg accagatccg cgctcgtcac gtagcggacg cgatactcga
     1501 tcgaacagag acgacgcccc actgcttcga tcaggtgact tttaccaagg cctgactggc
     1561 ccaccatgac caagttgtcg ccgcggcgta cgaactcgcc agcggccagt tcttcgaacc
     1621 ggccgcggtc gatcgtcttc tcgttgaact tccaatcgaa tgattccagc gttcccaggc
     1681 cggggaagcg ggcctcttga atgcggcgtt cgatgctccg ctctcgacga ccggacgcct
     1741 ggtcggcgat caggcaatgg acgaactgaa gatgcgacag gccttcctgc tcggcacgag
     1801 ccagggcggc gtcaagctgc tggtccgtca ggggaatctt cagagttcgg aagttctcca
     1861 ggagggcctg acggaggagg gccgggttct ctttcttcat caccggggtt ggcggggtcg
     1921 gctttggcgg ggtcagcttc ttcttgggca tgggattctt cgttggacaa cagttgttgg
     1981 taatcgcccg tggggcgcgg cggaaccgag gcgtctcgca acagatcggc cagatggtct
     2041 ttctcgcgat cggcgagcat ttccagcgcg gtcttgggct gggcctgaac atggagaatc
     2101 cgttccacgg cgtaaaagga gaacgcccca tagcgcagcg cccgttccag agccttgatc
     2161 agatcgcgat cgtggtagtg agcccgcagc gccagaatgt tgcgggcctg cgctttgcct
     2221 tggcgatggg ccttcagcag cccttcgaaa aaacgctctc cgacctctcc aaactcggcg
     2281 aagcttcccc gcagcagttc gtactgctgg cgtcggtcgt ctgggggacg atgggcggaa
     2341 tcgagctgac attgccggct tgcgctacgc ggcagcagcc gatgccgggc aatctcctcg
     2401 atctgagaac tgaagacgat caactcttcc tccgtgatgc gcacgggcag cacctgaccc
     2461 acgtagcgcc agggtacgga atactggttc tgacggtatt tgacgtaccc ttcgcaatcg
     2521 accgaacggt aaaccacctc cgccgcgtcg tagggatggt tcggcagcgg aatcaggtgc
     2581 ggctgctctt ccgcgtgcag atcgaccgga cgccgctggg tctgccggtg caggcgcacg
     2641 tcggcgactt ccgccagcca gcgcctggtc acttcattga gatgatccag cgagcggaac
     2701 tcgcggccgt tgagcaagtt catttccaca tagtggaacg gccgctccac tttccctttc
     2761 gtggcgggat ggcgcggccg gcacgctttg ggccgataac cgtagtgcgt ggcgaaagcc
     2821 agaaagcggg tgttgtaaac cggcagatcc gcctcgtagc gggacaccac gaccttcata
     2881 ttgtcgtaca aacagacggc agccacgccg gccagatgct cgaaggcctt cacgtgttgg
     2941 cgaaccgtcg tggcgaagtc ctggctttcc gtgaagcaga ggtactgccg gcgcgaatac
     3001 gccaagagat aactgaagag ataaacccgc cgacggcctt ccagcgtgaa gtccaggtcg
     3061 tactgggcat aatccatctg tgcttgcagt cccggccctg tttcaaagcg ctccaccaaa
     3121 ggctgcgcgg gttgcgggcg caactgggcc atccgttcct tgaggatgga gtagccgccc
     3181 tgaaagccgc gggcacgcag ttcttccagc atgcggacgg cggtcatgcg gggataacga
     3241 ccgagcagtt ggcgcatgac atcttcatgg tcgtccagca ggctcttgcg cttctgcttc
     3301 ggcgggggaa gggaagaagc cggcacctcg ccgttgcggg cctgatgatg gcctgccaac
     3361 gtccgacgca cccgctcacg gctgatctgc agatcgcgag cgatgcccct tgtcgattgg
     3421 ccttggtggt aacgtcgcac gatctcgtgt gtcaattctt ggttcatggc agatgcaact
     3481 ctcgcaagaa gtcctcggtc aattccgcaa cgccgcggca gtccaacagc agccgctcca
     3541 ggccgggccg caacggttcg cgatcccgca gttccagcac gccccgaccg ctgtagcgaa
     3601 gccacgactc caggccggct aagagatcga gcagcttggc cagtcgccgg gagaggtggt
     3661 tccccgcggc gctcatccgc gggtcccaac tgcgactgac gtgtccctcg aactcgcgca
     3721 gcgcctgacg cggcttctcc agcacgtacc gctgcttctc ctgagtcccc gacgccaaca
     3781 gcagatcgac cacgccgcga agttctgcgg ccgtgagcga ctctcgacgc acggtttcca
     3841 acgccttgag ctggttgccc gcgggcaacc gcgtcaattg ccgagccgcc gtcggcgaca
     3901 ccagccccag gcccagatct tcgcggacct ctggcgcgag cctctccagc agagccagcc
     3961 gccgacagac ccaactcttg tggcgcccca gcagttctgc caccgccggt tgcgacagcc
     4021 cgtcttcacg gatcagggca tgcacgatcc aggcttcttc cagggcttgc accgggcggg
     4081 cgagcgagtt cagcgtgtac acggcggctt tcgccgcggc tgcatccgcc tccaggcggc
     4141 gagaccagag cgtatccatg cctttcaccc gacccgcggc ggcgtgacgc ttgaagccgt
     4201 ccagcagcag aagacggcct tcggtcgcac agaccactat cggcgaaagc tggccgtacc
     4261 gtcgcaacga ctggaccatc gtttcttccg cctggcgatg caccaggcgg taacgatgga
     4321 aatccagatc aatctcggcc agaggcaggc aatcggtcga tccccattga tggctggcca
     4381 tcctcgactc cagtgtcatg cgcggcagcg gtgagcggca aagagcctct tctccatccg
     4441 cttttggaac ccgtgctgct cgttgcgttg tgtctcggcg aggagtttta gcgctggaag
     4501 tggcgactgc caatacagcg cacttaactc aaggccctcg taaagagacg accgcttgct
     4561 cgctcgtcgg ccacccttcg tcgcgccgcc aacgcgcttc ccgcagcagg actcgacgta
     4621 aagccagacg acacaacgct ccgcagaact tctgagcggg actgcgggcc gttgcgcgaa
     4681 acaacacgta gcaacccggc cgagagcagg cagatttttt cggcggcgcc tgcctgtgat
     4741 ggccctcgca ggccggcggc tccgccccag acgggacgct ttgaagcttc cgccgctccc
     4801 ggtaacggcg gcactgttca cgccggcact ggcgtccctg gtcgctccgc cggtaacgcc
     4861 ggccggctcg ccaccacgac cagcgccgcg ccgcggcttt gcaggacgaa ctgcaa
//
LOCUS       DASZYN010000309         9433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_331733, whole genome shotgun sequence.
ACCESSION   DASZYN010000309 DASZYN010000000
VERSION     DASZYN010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9433
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331733"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VHE33_08045"
     CDS             <1..661
                     /locus_tag="VHE33_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97444.1"
                     /translation="VKALSSGISVRTYERAMAYALLKEEFPQTRTLPEYPNKEALWAE
                     AIKATGGPQTPITYVEFGVYDAQGSFRWFLQNNTSPSSVFLGLDSFHGLPERFGKVGA
                     GYFDMHGVVPTVEDKRATLIEGWFKDTWDQLYKHIANRDNLLVHYDADIYSSTLFALA
                     RIELLRKPYVALFDEFTGPETLATYNYLKAFGAKIEMTGKVSVHGYPEQLLCRITPHG
                     A"
     gene            complement(872..1816)
                     /locus_tag="VHE33_08050"
     CDS             complement(872..1816)
                     /locus_tag="VHE33_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HVT97445.1"
                     /translation="MKTRLNCGSFTVLLVAASLALPAVAQSTSDQQPQVQTGASTNAQ
                     AQAQTTNPETGTGKAPLPMKSNEGFWGKVNPFARKKWVNRQLDPVKDRLNELDQLQAK
                     NANDIKDVDQRAQNGIRAAQSSADQANQVASAATTTAQQAQQTAQQASDKTNQLGETV
                     ANIDQYHPLTNTDIRFRPGQTVLNQKAKDALDQVVSQLQGQKGYVVEVAGYSRSRGTA
                     GLQNSQHMAEAVVRYLAEKQIPVWRIHEVAMGNTPISTDDNSTAKPGSIVRVSLMANS
                     LAALNQPSSGGSPIGATQQNSAQPSPQGAASQPTAQPQ"
     gene            complement(2258..2800)
                     /locus_tag="VHE33_08055"
     CDS             complement(2258..2800)
                     /locus_tag="VHE33_08055"
                     /inference="COORDINATES: protein motif:HMM:NF021163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2393 family protein"
                     /protein_id="HVT97446.1"
                     /translation="MSTPPSSDSGLFRPDRPGEREPRSPLPWIVALVAVLIVIGVLVV
                     VSRHEQPANPGGAGLAPPDPYAASLPITHLQMSQAGSMAGAQSTYIDGQIANTGQKTV
                     TGITVQVAFHGFENPIAKKETMPLALIRTRQPYVDTQSVAVAPIHPGETREFRLIFDS
                     LPQDWNQNYPEIRVIAVTGQ"
     gene            complement(3187..4572)
                     /locus_tag="VHE33_08060"
     CDS             complement(3187..4572)
                     /locus_tag="VHE33_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97447.1"
                     /translation="MKLVFRLALFAAAALPLSLSAATISGTVTDKTTGKPATGDTAVL
                     LDLQQGMQESAHTTIDAKGHYSFNVSDTAGMHLVRVDHDKASYYGPVPPNTTQVNIDV
                     FDVAAKVDGLRLYADVSRIETDQQGLSVTESYFIRNESKPPKTQLSAHSFEFYLPDGA
                     TLEGATATGPGGMATANQPVPAGEKGHYAFIFPIRPGETRFQVGYHLPYNGSAKLLAR
                     PTLPAENIAIMLPKSMSFDGGKNFQTLGSEASGPGTQTFLASNVKADTSVAFTVSGTG
                     SMPREQQNAQPGQGGMGGGQPGMEPGPDQTGQPAAQQGPGGGLGNPIDTPDPLQKYKW
                     WILSGVGLALVVGAAFMLRAKPGQAAALAPAEPSPLTSTLPPGVKATQVLAQKHAAPG
                     TAAVSVASTNGGAQKAAGTTGTLAALKEELFQLETERLEGKISDAEYAEHKSALETLL
                     KRALARESAAR"
     gene            complement(4592..4879)
                     /locus_tag="VHE33_08065"
     CDS             complement(4592..4879)
                     /locus_tag="VHE33_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97448.1"
                     /translation="MTTIACALLLVGAFLFVFWPERHVEAQHHKTRLEYLRERREVLY
                     DNLRDLNFEYKAGKYPEEDYAAQRTMLENEAAETVAEIELLESDALSLRHA"
     gene            complement(4882..5346)
                     /locus_tag="VHE33_08070"
     CDS             complement(4882..5346)
                     /locus_tag="VHE33_08070"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HVT97449.1"
                     /translation="MRRLMRFALLPLLVVVSVITLGAADTQARYQDLGHKLMCICGCS
                     EILIECNHVGCPDSDRMLAELRAAIDHGDSNNAILAAFQDKYGPTALAAPMLTKFNIV
                     AWIVPPALLVLGLFGTWILVKRWRMRMATMPVVEEAEMSDDLREKIRKETEV"
     gene            complement(5346..7415)
                     /locus_tag="VHE33_08075"
     CDS             complement(5346..7415)
                     /locus_tag="VHE33_08075"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HVT97450.1"
                     /translation="MPTFGTFALLVALALSGYNLLAGAVALRQLATGRRGAIAPERLA
                     ETARRAGIASFFATACAAFALVWAAFTNDFSVAYIAEHSNRALPGGYKFAALWSGQEG
                     SLLLWAFLLSAYGFVLRARHKVDVKLTAYASTILAGVQVFFLLLVNIAATPFSLVTGA
                     IPADGNGLNPLLQYPEMVIHPPMLYLGYVGFSVPFAFALGALMMRYPGEKWIHITRRW
                     TMVTWLFLTVGIFLGMHWAYNVLGWGGYWGWDPVENASLLPWLTGTAFLHSVMMQEKR
                     GMMKSWNVWLIFSTFMLSILGTLLTRSGLVSSVHAFAQSSIGNWFWIFLVFILAVCTF
                     TYIYHRDHLATEHKLESLVSRESSFLFNNLVLLAACFTVLWGTFFPVLSEYVQGNKVT
                     VSAPFYNRVAVPIGVFLLFLTGIGPILAWRSTSIKSIRRNFILPAIVGLIVAIGIIPL
                     GVHPWTIFTTDEQGAFYSWITFALAAFVATAIFNEFYKGARVIGQKSGQNLFAAMVQL
                     TRRNTRRYGGYIVHFGIVLIFVGIGGQAFNQSHEQTMNFKDSFNLGPYRVECLDYSQD
                     SNPNYDTEFAMLNVYRGDKLVTKLSPELRVYAVNGQPSTIVANHSTLIEDLYVIYAGK
                     DPATGQPVIKAFLNPLVSWIWIGLIVTIFGTLIALAPNMSATIAAARLQAHTPSEPPV
                     LAGGAGD"
     gene            7639..7998
                     /locus_tag="VHE33_08080"
     CDS             7639..7998
                     /locus_tag="VHE33_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HVT97451.1"
                     /translation="METRTYRDLLVWQRSMALVREIYAATEGFPRTEQFGVVSQLRRA
                     AVSVPSNIAEGRGRLTGKSFALVLSQARGSLYELQTQMELAGDLGFIGRERAQSVMSE
                     TAEIASMIQGLTVSLQK"
     gene            complement(8029..8412)
                     /locus_tag="VHE33_08085"
     CDS             complement(8029..8412)
                     /locus_tag="VHE33_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT97452.1"
                     /translation="MIADILLVDDNCIQATTRKAILARSGRNVAVACDGPQALAMLDQ
                     AGSRRPVGLVITDHLMPGMNGPEFVAELRTRFADLPVIVLSGLAEAEDEYRELDVLFR
                     VKPCPPDELLAVVRSQLDQPLSRTA"
     gene            complement(8675..>9433)
                     /locus_tag="VHE33_08090"
     CDS             complement(8675..>9433)
                     /locus_tag="VHE33_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97453.1"
                     /translation="LTMQHNLTWSKALGTGAVVQASSEYTPNDPFDLGAMYGEQPDDH
                     RILYNTYLVASEPWFKAQHGLLGRVAGGWTLAPIFTAGTGAPLYCNTLTDAQSFGSGD
                     GSSYFDNEQCVFTSQYKGGHSAHFGVTGSGGVGDSTANTAVNRFKDPESVFNQVRAPI
                     LGIDTKNPGVGPIMGQNFWNMDAEIKKNIRITETAVFEFSFIAANVFNHRQFSETQLD
                     ISDPGGWGVLSSQQSSIGGTPFTRQMEFGGRISF"
BASE COUNT         1873 a         2570 c         3179 g         1811 t
ORIGIN      
        1 cgtcaaagcc ctgtcttcag gcatcagcgt cagaacctat gagcgcgcga tggcctatgc
       61 gttgctcaaa gaagagttcc cccagacccg cacgctgccc gaatatccca acaaggaagc
      121 gctctgggcg gaggcgatca aggccacggg cgggcctcag acgccgatca cctacgtcga
      181 gtttggcgtc tatgacgctc agggctcctt ccgctggttc cttcagaaca acaccagccc
      241 ctcatcagtc ttcctcggcc tcgacagctt ccacggcctc ccggaacggt tcggaaaagt
      301 cggcgccggc tatttcgaca tgcacggcgt cgttccgacg gtcgaggaca agcgcgccac
      361 cctgatcgaa ggctggttca aggacacctg ggaccagctc tataagcaca ttgccaaccg
      421 cgacaacctg ctcgtccact acgacgccga catctactcg tcgacgctct ttgcgctggc
      481 ccgtatcgag ctgctgcgca agccgtacgt tgcgctgttc gatgagttca cgggcccgga
      541 gaccctggcg acctataact acctgaaagc cttcggcgca aaaatcgaga tgacaggcaa
      601 ggtgtcggtg cacggctatc cggaacaatt gctgtgccgg atcacgccgc acggggcttg
      661 aacgacatcg accggtggcg tctcctcgca agcgaggaaa gcccgtttgt tgaccgctga
      721 ccgctttttc gctgaccgct gtttagccga ccgattctta gctgaccgct tcttagctgc
      781 gccgcaggcg caaaaaaaag ggcccctcgg ggtgaggggc caatctgcct tggttctctc
      841 gttgtgtgag agctttttgg gccttgcctt cttactgagg ctgtgccgtt ggctgcgacg
      901 cagcaccctg aggggagggc tgcgcggaat tctgctgcgt cgcaccaatc ggtgaaccgc
      961 cagacgatgg ctggttcaag gccgcgagac tgttcgccat caggctcacc cgaacgatcg
     1021 agcccggctt cgccgtggag ttgtcgtccg tgctgatggg ggtgttgccc atggcgactt
     1081 catggatgcg ccataccggg atctgcttct cagccagata ccgaacgacg gcttccgcca
     1141 tgtgctgtga attctggaga ccggccgtac cgcgggagcg cgagtagccg gcaacttcaa
     1201 ccacgtaacc cttctggccc tgcaactggc tgactacctg atcgagggcg tccttcgcct
     1261 tctggttcag gacagtctgc ccgggacgga agcggatgtc ggtgttggtg aggggatgat
     1321 actgatcgat attggcaacc gtctcgccaa gctggttggt cttgtcgctc gcttgctggg
     1381 cggtctgctg tgcctgctga gccgtcgttg tggcggcgct ggctacctga ttcgcctggt
     1441 cggcggagga ctgggcagcg cggatgccat tctgggcgcg ctgatcgacg tccttaatat
     1501 cattggcgtt cttggcctga agctggtcca gttcgttcag acgatccttc accggatcga
     1561 gctgccggtt gacccacttc ttgcgggcaa agggattcac ttttccccag aacccttcgt
     1621 tggacttcat ggggagggga gctttgccgg tgccggtctc ggggttggtg gtctgggcct
     1681 gtgcctgagc gttggtggaa gcacccgtct gaacctgggg ctgttgatcc gaggtggact
     1741 gcgcgaccgc tggcaaagcc agacttgctg caaccagcag tactgtaaag gaaccgcaat
     1801 tcaatcttgt tttcatagtg tgtttgaact cggtatttga atttcggtgg gctgcgcagc
     1861 gccccgtatt gcacgaacct taaaagcact cgccgtgcca aagtgctcgg tcggggattg
     1921 aggcggataa gtgctttgga atcaaacgac gtggtactac ggggtgggtc atccgggcgt
     1981 tagtaacacg gtaaaaagca cagaatctgg caggatcggg aagaaattac gtcctgtcgg
     2041 aaggaaaaag gcatgccaaa ggctggccgg agacgagcca aacacgagcg aagggcgagc
     2101 caaaggcggg gggtcagggg cgagcccaaa agcgagccag gtaaaggccc gaaggccccc
     2161 gggcggttcg tccgaggcgt tcatctgcgc gtctcttgct cgccttcagc tcgtctgtag
     2221 ctcgtgtttc gctcgccgtt tgcttgatcc aggctcgcta ctgaccggtg accgcaatca
     2281 cccggatctc ggggtagttc tggttccagt cctgagggag gctgtcgaag atgaggcgga
     2341 attcgcgcgt ttcgccagga tgaatgggag ccacagcgac ggactgggta tcgacgtagg
     2401 gctggcgggt ccggatcagg gcgagcggca tcgtctcttt tttggcgatg ggattctcaa
     2461 agccgtggaa ggccacctgg acggtaatac cggtaacggt cttttgcccg gtgttggcga
     2521 tctgcccgtc aatataggtg gactgggcgc ccgccatgct gccggcctgg ctcatctgga
     2581 gatgggtgat gggaagcgag gcggcgtagg ggtcgggcgg ggcgaggccg gctccgccag
     2641 gattggcggg ctgctcgtga cggctgacca cgaccagcac cccgatgacg atgagcacgg
     2701 cgacgagggc gacgatccag ggtagagggg agcgaggctc acgctcgccg gggcgatcag
     2761 ggcggaagag tccggagtcg gaagacggag gcgtactcat gacggataca gggtacagcg
     2821 gatagcaaat agcggatagg tgagtctgct gaagaagtgt ttggcgacgc gcgaccggcc
     2881 acacgctcaa cgctatccgc tatacgctgt tctctttttc atcccgcaaa gttccgttaa
     2941 ctcatggagc actttcgcac agaggggcaa aaatatttct caagtttcga gtcgcacttg
     3001 ccgaacaatg agtctaagca ggcagaagtt cggccagcgg aatccccctt tgaggccatg
     3061 ctatgaacgt cctccccgaa gagccccaga gctcgaccag aaagccggag tacgcgtatc
     3121 ggatcgccgc tgcggcggcc gcattattcc tgttgctgac gtggatcagc gcgtaagcgc
     3181 gaggcgctaa cgcgcagcag attcgcgggc gagcgcgcgc ttgagtaaag tctccagcgc
     3241 tgatttgtgc tcagcgtatt cagcgtcgct gatcttccct tccagacgtt ctgtctccag
     3301 ttgaaacagt tcttccttca gagccgccag cgttccggtg gttcccgctg ccttttgcgc
     3361 gccgccgttg gtggaggcaa cgcttacggc cgctgtgccc ggcgccgcat gcttttgagc
     3421 cagaacctgc gttgctttga ctcctggagg cagcgtggaa gtgagcggag agggctcggc
     3481 gggcgcgagg gctgccgcct ggccgggttt ggcacggagc atgaaggccg caccgacaac
     3541 cagggcaagg ccgacgccgg acaggatcca ccatttgtat ttctgcagcg ggtccggcgt
     3601 atcgatcggg ttacccagtc cgccgccggg tccttgttga gccgccggct ggccggtttg
     3661 atcggggccg ggctccatcc cgggctgtcc gccacccata ccgccttgtc caggctgcgc
     3721 gttttgctgc tcgcgcggca tggaaccagt gcctgagacc gtgaacgcga ccgaggtgtc
     3781 cgccttaacg ttgctggcga ggaaggtttg cgttcccgga ccgctggctt cgctgccaag
     3841 ggtctggaag tttttgcccc catcgaaact catgctcttg ggcagcatga tggcgatgtt
     3901 ctccgcaggg agcgtggggc gcgccaggag ctttgcgctg ccgttgtaag gcaggtggta
     3961 accaacctgg aagcgggttt ctcccggacg gatcgggaag atgaacgcgt agtggccttt
     4021 ttcgcctgcg ggcacgggct ggttggccgt ggccatgccg ccggggccag tggcggtcgc
     4081 gccctcgagt gtcgcgccat cgggaagata gaactcgaag ctgtgcgcgc tgagctgggt
     4141 cttcgggggc ttcgattcgt tgcggatgaa gtagctttcc gtaacgctga ggccctgctg
     4201 gtccgtctca atgcgcgaga cgtcggcgta aaggcggagg ccatcgactt tggcagcaac
     4261 atcgaagacg tcgatgttga cctgggtggt gttgggcgga acggggccgt aataggaggc
     4321 cttgtcgtgg tcgacgcgaa ccaggtgcat gccggcggta tcggacacgt tgaaggagta
     4381 gtggcccttc gcatcgatcg ttgtgtgcgc cgattcctgc atgccctgct ggagatcgag
     4441 gaggaccgca gtatcgcccg tggcaggctt gccggtagtc ttgtccgtca cggtgccgga
     4501 gatcgtagcg gcactgaggg agagtgggag ggctgcggcg gcaaaaaggg cgagtcggaa
     4561 aacgagcttc atgggatcag gttccttgga tctaggcgtg acgcagcgat aaggcatcgg
     4621 attcgagcag ctcgatttcg gcgacggttt cggcagcttc gttctcgagc atggtgcgct
     4681 gcgcggcgta atcttcttcc ggatacttgc ccgccttgta ctcaaagttg aggtcgcgca
     4741 ggttgtcgta gaggacttca cggcgctcgc gcagatactc gaggcgggtc ttgtggtgct
     4801 gggcctcaac atggcgctcc ggccagaaga caaagagaaa ggcgccaacc aacagtaacg
     4861 cgcaggcaat cgtagtcatg atcagacctc tgtctccttc cggatcttct cgcgaagatc
     4921 gtccgacatc tccgcttctt caacgaccgg catagtggcc atgcgcatgc gccagcgctt
     4981 caccagaatc cacgtgccga acaggccgag gaccagcagc gcggggggca cgatccacgc
     5041 aacgatgttg aacttcgtga gcatcggcgc ggcgagtgcg gtcgggccgt acttgtcctg
     5101 gaaggcagcg aggatcgcat tgttggagtc gccgtgatcg atggcggcac gcagttcggc
     5161 gagcatgcgg tccgaatccg ggcagccgac gtggttgcac tcgatgagga tctcgctgca
     5221 gccgcagatg cacatcagct tgtggccgag atcctggtag cgggcctggg tgtcggcggc
     5281 gccgagagtg atgacggaga cgacgaccag cagcggcagg agcgcgaatc gcatgaggcg
     5341 gcgcatcagt cccccgctcc gccggcaaga acaggcggct ccgatggagt gtgtgcctgg
     5401 agtcgcgccg ccgcaatcgt cgcgctcatg ttcggcgcca gggcgatgag ggttccgaaa
     5461 atggtgacga tcaacccgat ccagatccac gagacgagcg gattgaggaa ggccttgatc
     5521 acgggctggc cggtggccgg atccttgccc gcgtaaatga cgtacaggtc ctcgatcaga
     5581 gtggagtggt tcgcgacgat cgtcgagggc tggccgttga cggcgtagac gcggagttca
     5641 ggggagagct tcgtgaccag tttgtcgccg cgataaacgt tgagcatcgc gaattccgtg
     5701 tcgtagttcg ggttcgagtc ctgcgagtag tcgaggcact cgacccgata cgggccgaga
     5761 ttgaagctgt ccttaaagtt catcgtctgc tcgtggctct ggttgaacgc ctggccgccg
     5821 atgccgacga agatcagcac gatgccgaag tggacgatgt atccgccgta gcgccgggtg
     5881 ttgcgacgcg taagctggac catcgcggcg aacaggttct gacccgattt ttgcccgatg
     5941 acgcgcgcac ctttgtagaa ctcattgaag atcgcggtag cgacgaaggc agcgagagcg
     6001 aaggtgatcc aggagtaaaa ggcgccctgc tcgtcggtgg tgaagatggt ccagggatgc
     6061 acgccgaggg ggatgattcc gatcgcaacg atgaggccga cgatggcggg aaggatgaag
     6121 ttgcggcgga tgcttttgat cgacgtggag cgccacgcga ggatggggcc gataccggtg
     6181 agaaagagca ggaacacgcc gatgggcacc gccacccggt tataaaacgg cgcgctgacc
     6241 gtgaccttgt tgccctgcac gtattcggag aggacgggga agaaggttcc ccagagcacc
     6301 gtgaaacagg cggcgagcag aaccagattg ttgaacagga agctggactc acgcgagaca
     6361 agcgattcga gcttgtgctc cgtcgcgagg tggtcgcgat ggtaaatgta ggtgaacgtg
     6421 cagaccgcca ggatgaaaac gaggaagatc cagaaccagt tgccgatgga agactgcgcg
     6481 aaggcgtgga cagaactcac gagacccgag cgagtgagca gcgttccgag aatcgacagc
     6541 atgaacgtgg agaagatgag ccagacgttc caggacttca tcatgccgcg cttctcctgc
     6601 atcatgaccg agtgcagaaa ggcggtgccg gtgagccagg gcaaaaggga ggcattctcg
     6661 accggatccc agccccagta gccgccccac ccgagaacgt tgtaggccca gtgcatgccg
     6721 aggaagatgc cgacggtcag gaacagccag gtcaccatcg tccagcggcg ggtgatgtgg
     6781 atccacttct cgcccggata gcgcatcatg agtgcgccaa gcgcaaacgc gaagggcacg
     6841 ctgaacccga cgtagccgag gtagagcatc ggtgggtgga tgaccatctc ggggtactgc
     6901 aacagtggat tgagaccgtt gccgtcggcc gggatggccc cggtgaccag cgaaaacggc
     6961 gtagccgcga tattgaccag cagcaggaag aaaacctgga cgccagcgag gatcgtggag
     7021 gcgtaggcgg tgagtttaac gtcgactttg tggcgggcac gaaggacgaa tccgtaggcg
     7081 gacagcagaa atgcccacag tagcagcgag ccttcctggc cggaccagag ggcggcaaat
     7141 ttatagcccc cgggaagggc gcggttggaa tgctcggcga tgtaagcgac gctgaagtcg
     7201 ttggtaaagg cggcccagac aagggcaaag gcagcgcagg cggtagcaaa gaaactggcg
     7261 attcccgcgc ggcgagcggt ttcggcaagg cgctcgggcg caatcgcacc gcggcggccg
     7321 gtggcaagct ggcgaagggc aaccgcgccg gccagcaggt tatagccgct gagcgcgagg
     7381 gcgacaagga gcgcaaaagt gccaaatgtt ggcataggga tcgaccttct gattgtcgca
     7441 ctttcgaaca cagggtgacg gaggcggcaa ccagggacag gattgatctt cgcgtgagac
     7501 cgggaggggg gatgtgatgg atgtcacggg ggaagggaga agggggaagg gagaaaaggg
     7561 gagaagcagt gactaaggtt gtgacaggag gccatcgtac tcttgcggga caagtcactc
     7621 agaatgaaat tgacagagat ggagactcga acctatcgag atctgctggt ttggcagcgc
     7681 tcaatggctc tcgtcaggga gatttacgcg gcgaccgagg gttttccgag gacggagcag
     7741 tttggtgtgg tcagtcaact ccgcagggct gcggtttcgg ttcccagcaa cattgcggag
     7801 ggacgcggac gcctgactgg gaaaagcttt gccctcgttc tatcccaggc tcgaggctcg
     7861 ctctacgagt tgcaaacgca gatggaactg gcgggagacc tgggctttat tggcagggag
     7921 cgtgcccagt cggtgatgag cgaaactgcg gagatcgcca gcatgattca agggctgaca
     7981 gtttccctcc agaagtagct tggcttccgg cttcttcctt ctcgctgctt aagccgtgcg
     8041 gcttagaggc tggtcgagct gggagcggac cacggcgagc agttcgtcgg ggggacaggg
     8101 cttaacgcgg aagagcacgt ccagctcgcg gtattcgtct tcagcttcgg cgaggccgct
     8161 gagaacgata accggcagat cggcaaaacg ggtgcgaagt tccgcgacaa actcagggcc
     8221 gttcatgccc ggcatcagat gatcggtaat caccagaccc acggggcggc ggctgccggc
     8281 ctggtcgagc atcgcgagag cctgagggcc gtcgcaggcg accgcaacgt ttctgccgga
     8341 gcgcgcgaga atggcctttc tggtggtcgc ctgaatacag ttatcgtcca ccagcaaaat
     8401 gtcggcaatc attgagatga agtgattccg tggaagagat ttaggttgcc ttgctcagga
     8461 gcccaatagt aaccgaagag gttccctcgt tctgtaatgc agatcacctt tgtacgcgtg
     8521 ggtaccaaac ccggcaagac caggtcaggg aatgatatat cccactttcg ttccagtgcc
     8581 atagaacagc agaaaaccga aggcggaggc ccgtggggac ctccgccttg agttgtgggc
     8641 tgcagccgga gaaccggccg caggctgggg ttggttagaa gctgatgcgg ccgccgaact
     8701 ccatctggcg ggtaaagggc gtgccgccga tggaggactg ctgagagctg agaacgcccc
     8761 agccaccggg atcagagatg tccagttggg tctcgctgaa ctggcggtgg ttgaagacgt
     8821 tggcggcgat gaaggagaac tcgaagaccg ctgtttcggt aattcgaatg ttcttcttga
     8881 tctcggcatc catgttccag aagttctgcc ccatgatcgg accgacgccg gggttcttgg
     8941 tgtcgatgcc caggatgggt gcgcgaacct gattgaagac cgattcaggg tccttgaacc
     9001 ggttgaccgc agtgttggca gtcgaatcac caactccgcc ggagccggtg acgccaaagt
     9061 gcgccgaatg cccgcctttg tactggctgg taaagacgca ctgctcgttg tcgaagtagc
     9121 tggagccgtc accggaaccg aaagactgag cgtcggtcag cgtattgcag tagagcggag
     9181 caccggtacc agcagtgaag atgggagcga gggtccagcc gccggccacg cgcccgagga
     9241 ggccgtgttg ggccttgaac cagggctcgc tggcaacgag gtacgtgttg taaaggatgc
     9301 gatgatcgtc cggctgctcg ccgtacatgg cgcccagatc gaacggatcg ttcggggtgt
     9361 attcgctgga ggcctggacg acggcaccgg tgccgagggc cttgctccag gtaaggttgt
     9421 gctgcatggt gag
//
LOCUS       DASZYN010000310         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_526086, whole genome shotgun sequence.
ACCESSION   DASZYN010000310 DASZYN010000000
VERSION     DASZYN010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526086"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            480..1711
                     /locus_tag="VHE33_08095"
     CDS             join(480..780,780..1711)
                     /locus_tag="VHE33_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612169.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HVT97454.1"
                     /translation="MRRKNEETGEERGRWSSKKKMAAVLRLLKGEDLDALSRELKVNA
                     ATLSSWREVFLANGLAGLKSREVDSRDEKITQLQKALGETALNLEISREINRVYERKT
                     RPFGPGEVEQVRRDVSTSQNRIVPLNRVCALAGLARSSLYFARRVDQSARGAPRGPTG
                     GHTDHELLGHIRAVLAASPFLGEGYRKVWAKLRHRGIRTSRPRVLRLMREHGLRAPTR
                     SGAPRGPRSHEGTIIPSAPDMRWGTDLTGTWTVRDGSVSVLLTVDHHTAEILGIHAAK
                     RATRWEALEPVRQAVRRCFGAIREQVAAGVELRHDNGSQYISDDFQDEIAFLGLRSSP
                     AFVRAPEGNGCVERAIRTLKEQLLWVKTFETVEELRLALIAWAELYNEQWLIERHGFI
                     SPAQRRRAYYAEAQSLAA"
BASE COUNT          351 a          597 c          629 g          344 t
ORIGIN      
        1 attgcgctgg ctcacgaatt cacgcacgct gttctttcac ctctcggaga gacggtgcca
       61 atggaaggcc ttcctatgca cgcgagcaag gaactggttc caggtcaacc tattgatgcc
      121 tacacgccag ccgccgtgta cggcccttcc cggcaaagga cctgcagtac aagacgacca
      181 gtcgtcctgc tcgggatgct cccgtgcctg gcgcctgcat cagaagaatt aaccgggcga
      241 tttacgtacg gtgagggtac aattggcatg cacgatgaag cctgctgcgc cggccggatt
      301 gttgtcgacg gactgttggc ggacgggtat gcgtacgcag ctctcgcgcg catacccgaa
      361 tcgcgaatgc ccgggttcgc tgcaaacaga atcaggaagt cgtttgcacc gctactcccc
      421 cggtatagag ttttcctgga cacgcttttg ggccaaagtg aaggcccgag gagtgtccga
      481 tgcgaagaaa gaacgaagag actggtgaag agcgaggccg ctggtcctcg aagaagaaga
      541 tggctgcggt gctgcgtctg ctgaagggtg aggacctgga cgcgctctcg cgggagctga
      601 aggtcaacgc ggcgacgctc tcgtcgtggc gggaggtctt cctggccaac gggctggcag
      661 gattgaagag ccgggaggtt gatagccgcg atgagaagat cacacagctg cagaaggcgc
      721 tgggcgagac agcgctgaac ctggaaatct cccgcgagat caaccgcgtg tacgagagga
      781 aacacggccc tttggccccg gggaagtcga acaagtgaga cgggacgtgt cgacttccca
      841 gaaccggatt gtcccgctga accgagtgtg cgctctggct ggcctcgctc gctcgagcct
      901 gtatttcgcc cgtagagtcg atcagtccgc ccgtggggcc ccgcggggcc ccacgggtgg
      961 acacaccgat cacgagctcc tcgggcacat tcgcgctgtc ctggctgcct cgccgtttct
     1021 cggggagggc taccggaagg tttgggcgaa gctgcgccac cgaggtatcc gcaccagccg
     1081 cccgcgggtg ctgcgcctga tgcgcgagca cggattgcgg gctccgactc gcagcggcgc
     1141 ccctcgcggg ccgcgctcgc acgagggcac aattattccg tcggcgccgg acatgcgctg
     1201 gggaacggat ctcaccggca cctggaccgt gcgcgatggt tctgtgagcg tgctgctcac
     1261 cgtcgatcac cacactgcgg aaatcctcgg catccacgcc gccaagcgcg ccacgcgctg
     1321 ggaggcgctc gagccggttc gccaggccgt tcgccgttgc ttcggcgcca tccgcgagca
     1381 ggtcgccgcc ggcgtggagc tgcgccatga caacggctcg caatacatca gcgatgactt
     1441 ccaggacgag atcgccttct tgggcctgcg ctccagcccc gccttcgtgc gagctccgga
     1501 gggcaacggt tgcgtcgagc gtgcgatccg taccctcaag gagcagctgc tgtgggtgaa
     1561 gaccttcgag acggtcgaag agctgcgctt ggcgctcatc gcgtgggccg agctgtacaa
     1621 cgagcagtgg ctgatcgagc gtcacggctt catatcgccc gcgcagcggc gccgtgccta
     1681 ctacgccgag gctcaatcgc tggctgcatg acagagttca ttaccaccaa ccgcgtgtcc
     1741 agaaaaccct tgaccgctac accccgaccg tagacgcgca gcgggcggca cgcttcttta
     1801 tcttgcgact gagggacttt gctgggcgcg gttcgtcgcc ctggtcgtga gcataatcgg
     1861 tgcgcgaggc gccgacgcgg gaacatggta cagcctcttg gtgtgtgccg ctgcaattcc
     1921 a
//
LOCUS       DASZYN010000311         1760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_526130, whole genome shotgun sequence.
ACCESSION   DASZYN010000311 DASZYN010000000
VERSION     DASZYN010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526130"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..997
                     /locus_tag="VHE33_08100"
     CDS             59..997
                     /locus_tag="VHE33_08100"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HVT97455.1"
                     /translation="MPIQATAKIWHNGNLIPWDKAQIHVMSHVVHYGSSVFEGIRCYT
                     QPEGAAVFRLPEHMQRMIDSARIYRMALGWSLDQLVSAVVETIEANGIAPCYIRPIAL
                     RGYGEIGVNPKGCPIDVYIANFPWGKYVAGDAADVCISSWNRLAPNTMPSLAKTGANY
                     ANSQLIRMEADVNGYVEGIALDVNGYLSEGSGENVFLVRNGVLYTTPLANSVLSGITR
                     DSVLTIARHFGIPVVEQAMPREMIYIADEVFFTGTAAEVTPIRSVDRIQIGDGEPGAI
                     TKQLRDEFFGIANGLKPDRFGWLTPVRVTAEQPVTA"
     gene            1167..>1760
                     /locus_tag="VHE33_08105"
     CDS             1167..>1760
                     /locus_tag="VHE33_08105"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HVT97456.1"
                     /translation="MAVGAAAAGRFARGLSFETQKIATAPLEEFGYGDVDLASDLHET
                     QLHETHAVLMALSDDSLLKPFRQMSGQPAPGEDLGGWYHYDPDWNDHRDWAGFAPGCT
                     FGQWVSALARGYAITGDRAVREKVLRLNRLWAQALTADVWVKNRFPAYTYDKILLGLL
                     DSHTLVGDPEALAILEQTTDVALPHLPGHAVEHGTRWR"
BASE COUNT          325 a          444 c          669 g          322 t
ORIGIN      
        1 ggcgcagggc ggacagatat aatcgacctt tcggagcgcg aaaggagacg gggagttcat
       61 gcccatccaa gcaaccgcca aaatctggca caacggcaac ctgatcccct gggataaggc
      121 ccagatccac gtgatgagtc acgtggtgca ctacggatcg tcggtatttg aaggcattcg
      181 ctgctacacg cagccggaag gggcagcggt atttcggctg ccggagcaca tgcagcggat
      241 gatcgattcg gcgcggatct accggatggc gctggggtgg tcgctggacc agctggtgtc
      301 ggcggtagtg gagaccatcg aggcgaacgg gattgctccc tgctatatcc ggcccattgc
      361 gctgcgcggg tacggggaga tcggcgtgaa cccgaaaggg tgcccgatcg atgtgtatat
      421 cgcgaatttc ccgtggggca agtatgtggc gggggatgcg gcggatgtct gcatttcgtc
      481 atggaaccgg ctggcgccca acacgatgcc gtcgctggcg aaaacgggag cgaactacgc
      541 gaactcgcag ctgatccgga tggaagccga tgtgaacggg tacgtcgagg gcatcgcgct
      601 cgacgtgaac gggtacctct ccgagggatc gggcgagaac gtgtttctgg tgcgaaacgg
      661 ggtgctatac acgacgccgc tggcgaattc ggtgctgtcg gggatcacgc gcgactccgt
      721 tttgacgatt gcgcggcact tcgggattcc tgtggtggag caggctatgc cgcgggagat
      781 gatctacatc gctgacgaag tgttctttac gggcacggcg gcggaggtga caccaattcg
      841 atcggtggac cggattcaga ttggcgacgg cgagccggga gcgatcacga agcagctgcg
      901 ggatgagttc tttgggattg cgaatgggct gaagccggat cggtttggct ggctgacgcc
      961 ggtgcgggtg acggcggagc agccggtgac cgcgtaggac tggcgagaaa gtaagaatgc
     1021 ccggctgcgt gccgggcatt tttgcgtccg tagatttgac ctggacgcgc tgcggagcta
     1081 tgcctgggga gtacgcatag ggggagtgga tgggctcgga gggacgggat cggatttcgc
     1141 ggaggcagtt tctcgaggcg ggatctgtgg cggtgggcgc tgcggcggca ggacgttttg
     1201 cgcggggtct cagcttcgaa acgcagaaga tcgctacggc gccgctcgag gaatttgggt
     1261 acggcgatgt ggatctggcg agcgatctgc acgagacgca attgcacgag acgcacgcgg
     1321 tgctgatggc gctgagcgac gacagcctgc tgaagccgtt ccgacaaatg tccggccagc
     1381 ctgctccggg cgaggatctg ggggggtggt accactacga tccggactgg aatgatcacc
     1441 gcgactgggc gggattcgcg ccggggtgca cgtttgggca gtgggtgtcc gcgctggcgc
     1501 gcgggtatgc gatcaccggg gaccgggcgg tgcgggagaa ggtgctgcgg ctgaaccggc
     1561 tgtgggcgca ggcgctgacg gcggatgtgt gggtgaagaa ccggttcccc gcgtacacat
     1621 acgacaagat tctgctgggg ttgctggact cgcacacgct ggtgggggat ccggaggcgc
     1681 tggccattct ggagcagacg acggatgtcg cgctgccgca tttgcctgga cacgccgtag
     1741 aacatggcac gcgatggagg
//
LOCUS       DASZYN010000312         4070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_526285, whole genome shotgun sequence.
ACCESSION   DASZYN010000312 DASZYN010000000
VERSION     DASZYN010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526285"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2914
                     /locus_tag="VHE33_08110"
     CDS             <1..2914
                     /locus_tag="VHE33_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HVT97457.1"
                     /translation="KIFQHFTQAKIAAPLSVGYGERGHYSKMEEMQLPVEAAGPMIPV
                     KFEAKELPGAAEGGAVVSWLGPGAGACVIDIEAAGSQDLVTFGPGPKTIWAYKNLHNG
                     SFEPIPSSQTGFNASGSGLACAVGDFDNDGFPDLAVALADRVVLYRNLGGGKFEDVTA
                     KVGIQQLNRPLGLTFVDFDHDGDLDLLVTGSASPSKGGPNVLWRNNGNSTFTEWTGPT
                     GLAGNGSTTQAMLSDINNDRAVDLVVTSDKGAPTVYLNQREGAFKALPLYTDASLRAT
                     RGVAVFDFDKDGWMDVAVTHAGSPGLTLWRNVDGEKFGRVPLPLPANVIDAFGVTAVD
                     FDNDGWIDLAAVLVTPQGSQVHLFRNLGPKGFKDVGSELGLDQLKLHNPGSLIAMDVD
                     GDGATDLVVTQAAAPPVVLRNVGGSRNHSLRIALAGLADNKTAIGTKVEVLAGGMWQK
                     FEVAGGGGYLSQGATEITAGLGQATKADVVRLLWPTGVPQDEINTDVTQLPHGELALK
                     ELDRRGSSCPTLFAWDGKKYQFVSDVIGAAVVGHWVSPTATNENDPDEWAKVDGDKLR
                     PRNGLLSLRFGEPMEEVNYIDQLRLVAVDHPAGTEVYPDERFLNERPFASGKTVAVSD
                     ARPVAGAWDDEGRDVRTLLSDKDHHYVRDFTNLSYAGFANMHALTLDLGAWSPQRPLR
                     LLLDGYIEYFSASSMYAAWQAGMQPIAPRVDVQLADGRWKRVIEDMGFPAGLPRTIVV
                     DLTGKLPPGARRIRISTNLQIYWDRVRVDNGRDAMPDVRQTNVPLAMAHLAFRGYPKQ
                     IEGATPGDLTYDYQQISATGPFLWARGVYTRYGDVTPLLKGIDNRYVIFGTGEEIDAE
                     FSAAGLPPLPAGWTRDYFFYANGFVKDMDYWEEAPFTVAPMPFQTMSRYPYPAKEHYP
                     QGPGADRYWLNWNTRYESGNRRQLWDFDYRPAKEQPDQGSNRGAQGTGSGVGPGMP"
     gene            complement(2945..3256)
                     /locus_tag="VHE33_08115"
     CDS             complement(2945..3256)
                     /locus_tag="VHE33_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97458.1"
                     /translation="MTLALIESAALVISPWVIGLVSSWHHPGGINSNDLPIPPGWLVV
                     IDLLGIVLAYAWYALYRRISARIAEGAPGYPFLDRIRLTMALLAQNVLALIILIAIEI
                     R"
     gene            complement(3399..>4070)
                     /locus_tag="VHE33_08120"
     CDS             complement(3399..>4070)
                     /locus_tag="VHE33_08120"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HVT97459.1"
                     /translation="RKARPRLAEGPLLESGLPALVAGEPETAADSPADHSSQRTRESE
                     SALPVSEPQPPAAPSWHSIHLDTETPIRQPRPSSPIHEDSALHAAPLEDRAMAALVDC
                     ALALCAFLLFSLVFALSSTHLPHGRIAVIGACAIVFAMGLLYQLLFFSLTDATPGMRY
                     AKIALCTFDDENPSRSAIRGRIAALVLSALPLGLGFLWALFDEDSLGWHDRITRTYQR
                     SYRDR"
BASE COUNT          822 a         1054 c         1462 g          732 t
ORIGIN      
        1 gaagattttc cagcacttca cgcaggcgaa gatcgctgcg ccgctcagcg tggggtatgg
       61 ggaacgcggg cactactcga agatggagga gatgcagctg ccggtagagg cggcggggcc
      121 gatgatcccg gtgaagtttg aggcgaagga actgccggga gcggcggaag ggggagctgt
      181 tgtcagctgg ctggggccgg gagccggggc gtgcgtgatc gacatcgagg cggcggggtc
      241 gcaggatctg gtcacatttg gcccggggcc gaagactatc tgggcataca agaacctgca
      301 caatggctca tttgaaccaa ttccttcgag tcagacgggc ttcaatgcga gcggatcggg
      361 tctggcgtgc gcggtgggcg atttcgacaa tgatggcttc ccggatctgg cggtggcgct
      421 ggcggaccgg gttgttctct atcgcaatct tggcggcggg aagtttgaag atgtgacagc
      481 gaaggtgggg atccagcagc tgaaccgccc gttgggcctg acgtttgtgg acttcgatca
      541 tgatggggat ttggacttgc tggtgacggg atcagcttcg ccgagcaaag gcgggcccaa
      601 cgtgctttgg cgaaacaacg gcaactcgac gttcacggaa tggaccgggc cgacggggct
      661 ggcggggaat ggcagcacga cgcaggcaat gttgtcggac atcaacaacg atcgcgccgt
      721 cgatctggtg gtcacgtcgg acaaaggcgc gccgaccgtt tatctgaacc agcgggaggg
      781 ggcgttcaaa gctctaccgc tgtatacgga tgcgagtttg cgggcgacgc gcggtgttgc
      841 ggttttcgat ttcgacaaag acggatggat ggacgttgct gtgacccatg ccggttcgcc
      901 agggttgaca ctgtggcgca atgttgacgg cgaaaaattt gggcgggtgc cgttgccgct
      961 tccggcaaac gtcattgacg cgtttggggt cacagcggtt gatttcgata atgatggctg
     1021 gattgatctg gctgctgtcc tggtgacccc tcaggggtct caggtccatc tatttcgcaa
     1081 tctcgggccc aagggattca aagatgtcgg ctccgagttg ggactcgacc agctgaagtt
     1141 gcacaaccca ggttcgctga tcgcgatgga tgtcgatgga gatggtgcga cagaccttgt
     1201 cgtgacacag gcggctgccc cacccgtggt gctgcgcaac gtcggcggca gcaggaatca
     1261 ttcgctgcgg attgccctgg ctggcctcgc cgacaacaaa accgcgatcg gaacgaaggt
     1321 cgaagtgctg gccgggggga tgtggcagaa gtttgaagtg gccggtggcg gcggttatct
     1381 cagccagggg gccacagaaa taaccgccgg gctggggcag gcgacgaagg ccgacgtggt
     1441 gcggctgctg tggccgaccg gcgttccgca ggacgagatc aacacggatg tgacgcagct
     1501 tccgcacggg gaactggccc tgaaggagct ggaccggcgg ggcagctcgt gtccgacatt
     1561 gtttgcctgg gatgggaaga agtatcagtt cgtgtcggac gtgattggcg cggcggtggt
     1621 ggggcactgg gtttcgccga ccgcgacaaa cgagaacgat ccggacgagt gggccaaggt
     1681 ggatggagac aagctgcggc ctcggaatgg cctgctgagt ctgcggttcg gcgagccgat
     1741 ggaggaggtc aactacatcg accagctgcg actggtggcg gtagatcatc cggcaggcac
     1801 ggaagtctat cccgatgaac ggttcctgaa cgagcggccg tttgcgtcgg ggaagaccgt
     1861 agccgtgtca gacgctcggc ccgtggcagg agcctgggat gatgaggggc gggacgtgcg
     1921 aacgctgttg tccgacaagg atcaccacta cgttcgggac ttcacgaatc tgagctatgc
     1981 cggtttcgcc aacatgcacg cgctgacgct cgacctggga gcgtggtcgc cccagagacc
     2041 gctgcggctg ctgctggatg ggtatatcga gtacttcagc gccagctcga tgtatgcggc
     2101 ctggcaggca gggatgcagc ccatcgcccc acgcgtggat gttcagctag ccgacggccg
     2161 ctggaagcgc gtgatcgagg acatgggatt cccggcgggg ctgccgcgaa cgattgtggt
     2221 ggatctgacg gggaagctgc ctccaggcgc gcgaaggatt cgaatctcga cgaacctgca
     2281 gatctattgg gatcgggttc gcgtggacaa tgggcgagac gccatgcccg acgtgcgtca
     2341 gacaaacgtg ccgctggcga tggcgcatct ggcgttccgg gggtatccga agcagattga
     2401 aggcgccaca ccgggcgatc tgacctacga ctaccagcag atcagcgcga cggggccatt
     2461 cctgtgggcg cgcggcgtgt acacgcgata tggcgatgtg acgccgctgc tgaagggaat
     2521 cgacaaccgt tacgtgatct tcgggacggg cgaggagatc gacgcggagt tcagtgccgc
     2581 tggattgccg cctctgccgg cgggctggac gcgggactac ttcttctacg caaatggctt
     2641 tgtgaaagac atggactact gggaagaggc tccgtttacg gtagctccga tgccattcca
     2701 gaccatgagc cgatatccgt acccggcgaa agaacattat ccacagggac caggggcgga
     2761 ccggtactgg ctcaactgga atacgcggta tgagtcgggg aaccggcggc agttgtggga
     2821 tttcgattat cgtccggcaa aagaacagcc ggatcagggc tcaaatcgcg gagcgcaggg
     2881 cacaggaagc ggcgtgggac cgggcatgcc gtagcggctg cccggcttcc agcaggtcta
     2941 aaacctagcg aatctcgatg gcgatgagaa tgatcagggc gagcacattc tgcgcgagca
     3001 gcgccatggt gagccggatt cgatcgagga agggatagcc gggagcccct tccgcaattc
     3061 ttgcgctgat acgccgatac agcgcgtacc acgcatacgc cagcacgatc ccaagcaaat
     3121 cgatgacaac aagccatccg ggtgggatgg gcagatcgtt ggagttgatg ccgccgggat
     3181 gatgccagct ggataccaga ccgatcaccc agggagagat caccaaagca gcggattcaa
     3241 ttaaggcaag agtcatgtag atccgggcgt ggccctcgaa ctgaccgtgc caggggccgg
     3301 actcggacgc agcggtcgtc attgttgtag tggtcacaac agagtgggat gcgaatttgc
     3361 cgtgaaaagt cgtggctcta aggggagtta ctgaagggct atcgatcccg gtaactgcgc
     3421 tggtacgtgc gggtgatccg gtcgtgccat ccgagagagt cttcgtcgaa gagggcccag
     3481 aggaacccga ggccgagggg gagcgcggag aggacgaggg cggcaatgcg gccgcggatg
     3541 gcggatcgtg aggggttttc gtcatcgaag gtgcaaagtg cgattttggc atatcgcatg
     3601 cccggcgtgg cgtcggtgag gctgaaaaac agcaattggt agagcaaacc catggcaaaa
     3661 acgatggcgc aggcgccgat cacggcgatg cggccgtgcg gcaaatgggt gcttgagagc
     3721 gcaaaaacca gcgaaaaaag caaaaaagcg cacagggcga gcgcgcaatc caccagggcc
     3781 gccatcgcgc ggtcttcgag cggcgcggcg tgaagcgcac tgtcttcatg aattggggag
     3841 gacggcctgg gctggcggat gggggtctcc gtgtcgaggt gaatggagtg ccagctcggt
     3901 gcggccggtg gctgcggttc cgaaaccggc agggcggatt ccgattccct ggtccgctgt
     3961 gacgaatggt ccgcaggtga gtctgccgca gtttcgggct ctccagctac cagagcgggt
     4021 agtcccgact ccaggagcgg accttcggcg aggcggggac gcgccttacg
//
LOCUS       DASZYN010000313         1996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_76386, whole genome shotgun sequence.
ACCESSION   DASZYN010000313 DASZYN010000000
VERSION     DASZYN010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76386"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VHE33_08125"
     CDS             <1..303
                     /locus_tag="VHE33_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVT97460.1"
                     /translation="KADYRHGEESDPEEKTVQLAAREAGPERELLSREIQAAQEMAMR
                     RLTPVEKTAFVLRHLEDRSMGEIAEVLGIQPNAAKQAVFRAVQKLRRELAPLRGTA"
     gene            300..1064
                     /locus_tag="VHE33_08130"
     CDS             300..1064
                     /locus_tag="VHE33_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97461.1"
                     /translation="MKHLNEEELIELYYGESNAGNVAHLAGCQECSVQMAELKKDLEG
                     ARMAVPERGAEYGEQVWQELRPRLIPYEKRALGWRGFFGKSGAQWKVAVIAVGCAALV
                     AAAFLGGRVWERHQKTNVAGGANPEVKQRVVLVVLTDHLDRTERLLVSLEHANSSDRV
                     ENAQLQSEARELLASNRLYRTTATNAGDPVLAGALDKLQGVLAEVANDPNLTAADLER
                     LRSEMNTEGILFEIRVLRARGAEEMSGQKSAKGAAI"
     gene            1064..>1996
                     /locus_tag="VHE33_08135"
     CDS             1064..>1996
                     /locus_tag="VHE33_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HVT97462.1"
                     /translation="MKTQRWVLILLAAAVVVAPWRLHAWAAQPAIAIALGSGGAGIGA
                     DDANLYADGTRAINESRWQDAAGLFGRVADMRGEHAEGALYWKAYAENKEGQPASALN
                     TCGQLRKSYPKSRWLNECGALEIEIRGKSGDPVAPQAEADENLKLLALNALMQQDQKE
                     AVPILQKILAGNQSEELKSRALFVLAQNQSPESQAVIKQIAQGQSGPALQIRAIRMMA
                     TQGKRANDTLADIYQHTSDKQVKRAILQSYLVSGDPEKELAAARTETDPELVKTAVHT
                     LGAMGAAQDLMTLYRATNNAEAKADIINSFIPAGK"
BASE COUNT          412 a          474 c          801 g          309 t
ORIGIN      
        1 aaggccgatt accggcacgg tgaagagagc gatccggaag agaagaccgt gcagctggcg
       61 gcgcgcgagg cagggccgga acgggagttg ttgagccgcg agatccaggc ggcgcaggag
      121 atggcgatgc ggagactgac gccggtggag aaaacagcgt ttgtgctccg gcatctggag
      181 gaccgatcga tgggcgagat tgccgaggtg ctggggattc aaccgaacgc ggcgaagcag
      241 gcggtgtttc gcgcggtgca gaaattgcgg cgggaactgg cgccactgcg ggggacggca
      301 tgaagcattt gaacgaagag gagctgattg aactgtatta cggcgaaagc aatgccggga
      361 atgtggcgca tctggcaggg tgccaggagt gctcggtgca gatggcggag ttgaagaagg
      421 accttgaagg tgcgcggatg gcagttccgg aacggggagc ggagtacggc gagcaggtgt
      481 ggcaggaatt gcggccgcgg ttgattccct atgagaagag agcgctgggc tggcggggat
      541 ttttcgggaa gagcggggcg cagtggaagg tagcggtgat cgcggttggc tgtgcggcgc
      601 tggtggcagc ggcgtttctg ggtgggcgtg tttgggagcg gcaccagaag acgaatgtgg
      661 cgggtggtgc gaatccagag gtgaagcaga gggtcgtgct ggtggtgctg acggatcatc
      721 tggatcggac ggagcggctg ctggtttcgc tggagcatgc caactcctcg gatcgggtgg
      781 agaatgcgca gctccagtcc gaggcgcgtg aactgctggc ctccaaccgg ctgtatcgga
      841 cgacggcgac gaatgccggc gatccggtgc tcgctggggc gctggacaag ctgcagggcg
      901 tgctggccga ggtagcgaac gatcccaatc tgacggcggc ggatctggag cggctgcgca
      961 gcgagatgaa tacggaaggg attttgtttg agatacgcgt tctgcgggca cgcggggcgg
     1021 aagagatgag cgggcaaaag tccgcaaagg gagcggcgat ctgatgaaaa ctcaacggtg
     1081 ggttctgatt cttttagcag cggcggttgt ggttgcgccg tggagactgc atgcctgggc
     1141 ggcgcagccg gcgattgcga ttgcgctggg atcgggtggc gcgggcatag gcgccgacga
     1201 cgcgaacctg tatgccgacg ggacgcgggc catcaacgag agccgctggc aggatgcagc
     1261 gggcctgttc ggacgcgtgg cggacatgcg gggcgagcac gcggagggcg cgctctactg
     1321 gaaggcttat gccgagaaca aagagggaca gccggcgagc gcgctgaata cgtgcgggca
     1381 gctgcgcaag agctatccga agagccgctg gctgaatgag tgcggggccc tggagattga
     1441 gattcgcggc aagagcggcg atccagtggc gccgcaggcc gaggccgacg agaacctgaa
     1501 gctgctggcg ctgaatgcgc tgatgcagca ggaccagaag gaagcggtgc cgatcctgca
     1561 gaagatcctg gcagggaacc agtccgaaga gctgaagagc cgggcgctgt ttgtgctggc
     1621 gcagaatcag tcgccggagt cgcaggcggt gatcaagcag attgcgcagg ggcagtcggg
     1681 accggcgttg cagatccggg ccatccgcat gatggcgacc cagggcaagc gcgcaaacga
     1741 cacgctggcg gacatttatc agcacacgag cgacaaacag gtgaagcggg cgatcctgca
     1801 gtcgtatctg gtgagcggag atccggagaa ggaactggcg gcagcgcgga cggagaccga
     1861 tccggagctg gtgaagacgg cggtgcatac gctgggcgcg atgggagcgg cgcaggatct
     1921 gatgacgctg tatcgcgcga cgaacaatgc agaggcgaag gcggacatca tcaattcgtt
     1981 cattccggcc gggaag
//
LOCUS       DASZYN010000314         2404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_975325, whole genome shotgun sequence.
ACCESSION   DASZYN010000314 DASZYN010000000
VERSION     DASZYN010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975325"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VHE33_08140"
     CDS             complement(<1..286)
                     /locus_tag="VHE33_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97463.1"
                     /translation="MLAQEDEVLAELILRQGGRVALKVFGELADVTDVFLFGRLAEIF
                     KLDVLLEFGDRRIISFDHRPGRMPACAVNFPANLKTPYGTAHFLPRSGSVQ"
     gene            308..1018
                     /locus_tag="VHE33_08145"
     CDS             308..1018
                     /locus_tag="VHE33_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVT97464.1"
                     /translation="MKMAKYALLCFYVECLKVTGWTVFREVRVAEPQVLPAVLSRSEV
                     KALLATIREPRFRACLTLMYHCGLRVGEAVRIQIKDIQARQDPPRLLVRNGKGGKQRY
                     VPLSMPMVRELREWWKTHQNPALLFPSPGRGWADRTLTLSQAMHLSKEPMSESCVQAA
                     YRMGRAASGINPASTTHTLRHSYATHLLEEGVSLRQISSYLGHESLDTTVIYTHLTSV
                     SEARTQAALGKLYNNLKS"
     gene            1055..2104
                     /locus_tag="VHE33_08150"
     CDS             1055..2104
                     /locus_tag="VHE33_08150"
                     /inference="COORDINATES: protein motif:HMM:NF033538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HVT97465.1"
                     /translation="MLRRYWPQYERQFGSALLHSHRRAVQAILSCRTSALGGQIYRCA
                     DCRRDRFVYHSCNHRACPQCGSADAAEWVEEQKRKLLPVPYYLITFTVPEGLRQWLRS
                     HQRQGYAALLRESAATLQDVAANPKYLGAELGLLAVLHTWGRQLQFHPHVHCVVPAAG
                     LTADRLRWRRPPSADFFLPQAVLAARFRTRLKAALLPLPQSAAIGKPVWHQKWVVDVQ
                     PAGSGETAIKYLAAYVYHTALGSQRILNADHGHVTFKYKDSSDQQWRALRLTANEFLR
                     RFLQHVLPKGLQRIRYFGWLAPAAKARWQRILTLLDYKEPPRILIERSAPACPCCQKP
                     MRLLAELPRPPPLRT"
BASE COUNT          506 a          813 c          615 g          470 t
ORIGIN      
        1 gttgaactga gccgctgcgc ggcaggaagt gggcagttcc atacggtgtt ttgagattgg
       61 ccgggaagtt gaccgcacac gcgggcatcc ttcccggcct atgatcaaaa ctaattatcc
      121 ttctatcacc aaattccagc aacacgtcga gcttaaagat ttccgcaagc cgaccaaaaa
      181 ggaatacgtc cgttacgtcc gcaagctcgc cgaacacttt caatgcgacc cggccacctt
      241 gacggaggat cagctccgcc agtacttcat cttcctgcgc gagcacaagc gctactcccg
      301 cagcccaatg aagatggcca aatacgcgct gctctgtttc tacgttgaat gcctcaaggt
      361 taccggctgg accgtctttc gcgaagtccg cgtggccgag ccccaggtat tgcccgccgt
      421 gctctcgcgc tccgaagtca aagcgctgct tgccacgatc cgcgaacccc gctttcgcgc
      481 ctgcctcacc ctcatgtacc actgcggcct tcgcgtcggc gaagccgttc gcatccaaat
      541 caaagacatc caggcgcggc aagacccgcc tcgcctgctg gtgcgcaacg gcaaaggcgg
      601 caagcaacgt tacgttccgc taagcatgcc catggtgcgg gaactgcggg agtggtggaa
      661 aacgcatcaa aacccggcgt tactctttcc cagtccgggc cgcggctggg ccgaccgcac
      721 gctcactctg agccaggcta tgcacctgag caaagagccg atgagtgaaa gctgtgttca
      781 agccgcttac cggatgggcc gcgctgccag cggcatcaac ccggcctcca cgactcatac
      841 actgcgtcac agttacgcca ctcatctgct cgaagagggc gtgtccctgc gccagattag
      901 ctcctatctc ggccatgaat cactcgacac caccgtcatc tacacgcacc tgaccagtgt
      961 cagcgaggcc cgcacacagg ccgctctcgg caagctctac aacaatctca aaagctagaa
     1021 cccgccgctt tcgctggtga tagctctggc cgaagtgctg cggcgatact ggccgcagta
     1081 tgaacggcag tttggttccg cgctgctcca ctcccatcga cgcgccgtgc aggcaatcct
     1141 gagttgccgc acctccgccc tgggcgggca gatttatcgc tgtgccgact gccgccgcga
     1201 tcgctttgtc tatcactcct gcaatcaccg cgcctgccct caatgcggca gcgccgatgc
     1261 cgccgagtgg gtcgaggaac aaaaacgcaa actcctgccc gttccctatt atctcatcac
     1321 cttcacggtg cctgaaggac tgcgccagtg gttgcgatca catcagaggc agggttacgc
     1381 ggctctgttg cgcgaaagcg ccgccacact tcaggacgtt gccgccaatc caaaatatct
     1441 cggcgctgaa cttgggctgc tggccgtgct gcacacatgg ggacgacagt tacagttcca
     1501 tccgcatgtc cactgcgtcg tcccggctgc aggcttaacc gccgaccggc tacgatggcg
     1561 ccgtccgcct tcggccgatt tttttctgcc acaagcggtc ctggccgccc gtttccgaac
     1621 acggctcaaa gccgcgctgc tgccgctgcc acagtcggca gcgatcggca aaccagtctg
     1681 gcatcagaaa tgggtcgtcg atgtgcagcc tgccggtagc ggagaaacgg ccataaaata
     1741 tttagccgca tatgtctatc acaccgcgct gggttcacaa cgcatcctca acgcggacca
     1801 tggccacgtc acctttaagt acaaagacag cagtgaccag caatggcgcg cgctccggct
     1861 caccgccaat gagttcctcc gccgcttctt gcagcatgtc cttcccaagg gcttgcagcg
     1921 catccgctat ttcggctggc tcgctcccgc agccaaagcc cgctggcaac gcatcctcac
     1981 actgctcgat tacaaggaac ctccaaggat tctcattgag cgttccgcac cagcctgccc
     2041 ctgctgccaa aagcccatgc gcctgctggc cgaactgcca cgaccgccgc cgctcagaac
     2101 atgatccaaa cagactttaa acatggatcg gctcgccagc ggaaaaattg cgttgcgcgg
     2161 caacgtgcgg ccttgtcctc aggccgaaaa atgtcgctcg aaaaggctgc aacctcatgc
     2221 ccgcgatgcg gaatcacgct cgcacgatta acaccacact tagcccgccc ttatttttta
     2281 gttccgccgt tccgaaatcc tgcctccggc tgaaaaaaca aatcgcaaga ctcgcaaaac
     2341 ttcccgggct cgttcaacca ctgatagcat tcaggcttcg ctgaatgcta tcttggccgt
     2401 tagg
//
LOCUS       DASZYN010000315         3584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_721518, whole genome shotgun sequence.
ACCESSION   DASZYN010000315 DASZYN010000000
VERSION     DASZYN010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721518"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3327
                     /locus_tag="VHE33_08155"
     CDS             <1..3327
                     /locus_tag="VHE33_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT97466.1"
                     /translation="QLEPAHYKLTVTHEGFQAKTLEQVQITPEQANTIDVQMQIGAAT
                     QTVRVSSLTRGLPTDTATVSATISSNQIQHLPSFNRDVFQLAQLTPGVFGDASRSSGG
                     GASTLPGNQGPGGTSAGAGGIFSTENGPQIQTRGGQYETNGISIDGISTTSAVWGGTS
                     IITPSEDSVDSLHVVSNAYDAQYGRFSGGNLEVTTKSGTNNLHGSAFFKASRPGLNAY
                     QRWNGTGSNKAPTGATNAARAASRGVNRDESRFNQYGGSLGGPLWKDRLFAFFNYETS
                     PLNSKSTSQGWYETSEFDSSAAPTSSIAAKYLGTKGEGVASNGMVARTCASAGLTEGV
                     NCATVSGGLDLGSPLTSGLGQQDLSYGGNTNNPGVGGGLDGVPDLAYFNTVNPTSTSQ
                     AQYNGRLDANVTGSDHLAFAIYWVPVSTTNYQGPVRSANLWHHSQVNDAVSLIWNHTF
                     SPTLLNQARANAAGWRWNEITSNPQEPFGLPQDNIDALGATSAEGGVQAFGAPGPSNL
                     NQWTYSYNDVLTKVWGRHSIKAGGELTRLYYLNNAVYAARPQFNFHNLWDFANDAPYH
                     ESGTFDHATGIPFSNREDNRQNLWGFFVQDDWKVKPNLTINAGLRWSYFGTFYSKQNN
                     LDIMRFGSASDPLGGLNIKVGGGLYSPQKSNWGPQIGFAWQPKSQEGKAVLRGGFGIN
                     YNQNEIAILANGFGNPPNAVNASFNCDYPAFLTNPGCSGTGILYETATNTNSIFGYAP
                     NPAAITTFSSANLPLTGTTSVTGFPANPKTITNYHYSLEADYQLPFNTVATLGYQGSL
                     MRHLLVQNDWLAIAAQRGLPMNPVVNNINYYDNAGNANYNAMIATLTHNFDHGFQAEA
                     QYTWQKSLDENSGPYSEDPYPYDVHAAYARSDYNVPNAVKLFGLWQPMFFHGHNFAEK
                     VVGGWSLSGIWNWHTGFPWNPTYTTTSNVYLANSPYSSLRPAGIRKGFGTNVSNKSFE
                     GIESANKNPNFGGDGTPFFPGPTYTPGGSFPASSPGPPPGIQRNSLNGPHYSDLDASL
                     TKAFGLPNNKVLGENARVEVRADAYNLFNQTNLNPTSIDGNLGSVGPDGSITSVNGDF
                     GIARGALGSRTVQMQARFSF"
BASE COUNT          791 a         1019 c         1132 g          642 t
ORIGIN      
        1 caactggagc cggcgcacta caagctgacc gtgacccacg agggtttcca ggcgaaaacg
       61 ctggaacagg tgcagatcac gccggagcaa gcgaacacca ttgatgtgca gatgcaaatc
      121 ggcgctgcga cccagacggt gagggtgtcg tcactaacac gcggcctgcc cacggacacg
      181 gcgacggtga gcgcgaccat tagcagcaat cagattcagc accttccatc gttcaatcgc
      241 gatgtcttcc agctggcgca gttgacgcct ggagtgttcg gcgacgcatc gcgatcgagc
      301 gggggcggag cttcgacgct accgggcaat caggggccgg gcggcacatc cgcgggcgcg
      361 ggcggcattt tctcgaccga gaacggaccg cagatccaga cgcgcggcgg gcaatacgag
      421 acgaacggga tcagcatcga cggcatcagc accacgagcg cggtatgggg cgggacttcg
      481 atcatcacgc cgagcgagga ctcggtggac agcctacacg tggtttcgaa tgcgtatgac
      541 gcgcagtacg gccggttcag cggcggaaac cttgaggtga cgacgaaaag cggcaccaac
      601 aacctgcatg gcagcgcctt cttcaaggca tcgcgacccg ggttgaatgc gtatcagcgg
      661 tggaatggca cgggatcgaa caaagcgccg acgggggcca ccaacgcggc tcgggctgcg
      721 tcgcgcggcg tgaaccgcga cgaatctcgg ttcaaccaat acggcggaag cctgggcgga
      781 ccgctctgga aggaccgcct ctttgcgttt ttcaattacg agacgagtcc tctgaactcg
      841 aagtcaacgt cgcagggatg gtatgagacg tcggagttcg attcgtcggc cgcaccgacg
      901 agcagcattg cggcgaagta tctgggcacc aaaggtgaag gtgtggcgtc gaacggcatg
      961 gtggcgcgaa cctgcgcttc ggccggacta acggagggtg tgaactgcgc aactgtgagc
     1021 gggggactgg atctggggtc gcctctaacc tcaggtctcg gtcaacagga ccttagctac
     1081 ggtggcaaca cgaacaatcc gggcgttggt ggcggcctcg acggggtgcc ggatctggcg
     1141 tacttcaaca ccgtgaatcc aaccagcacg tcgcaggcgc agtacaacgg acggctcgat
     1201 gcgaacgtga cgggctcgga tcacctggct tttgcgatct actgggttcc ggtgagcacg
     1261 accaactatc agggaccggt gcgctcggca aacctgtggc accactcgca ggtgaacgat
     1321 gcggtttcac tgatctggaa tcacacgttc tcaccgaccc tgctcaacca ggcgcgtgcc
     1381 aacgcggcgg gctggcggtg gaacgagatc acgagcaatc cacaggagcc gttcggactg
     1441 ccccaggaca acatcgatgc actgggtgcg acgagcgcag agggtggggt ccaggccttc
     1501 ggcgcaccgg gaccgagcaa tctgaatcag tggacctact cctataacga tgttttgacg
     1561 aaggtgtggg ggcggcacag cattaaggca ggcggcgagt tgacgcggct ctactacctc
     1621 aacaatgctg tctatgcggc gcggccacag ttcaacttcc acaacctgtg ggattttgcc
     1681 aacgacgctc cgtatcacga gagcggaacc ttcgatcacg cgaccggcat tcccttctcg
     1741 aatcgtgaag ataatcggca gaatctgtgg ggattcttcg tacaggacga ctggaaggtg
     1801 aagccgaacc tgaccatcaa cgccgggctg cgctggtcct actttggcac gttctactcg
     1861 aagcagaaca acctcgacat catgcggttt ggcagcgcct cggatccgct gggcggcctg
     1921 aacatcaagg tgggcggcgg tttgtactcg ccgcagaaga gcaactgggg accgcagatc
     1981 gggttcgcct ggcagccgaa gagtcaggag ggaaaagcag tgctgcgcgg cggattcggg
     2041 atcaactaca accagaacga gatcgcaatt ctggcgaatg gttttggcaa tccgccgaat
     2101 gccgtcaacg caagcttcaa ctgcgactat ccggcgttcc tgaccaaccc agggtgcagc
     2161 gggacgggca ttctgtacga gacggcgacc aataccaact ccatttttgg gtacgccccg
     2221 aatcccgcag caatcacgac attcagcagc gccaatctgc cgctgaccgg cacgacttcg
     2281 gtaacaggct tccctgccaa tccaaagacg atcacgaact accactactc gctcgaggcc
     2341 gattaccagc taccgttcaa cacggtcgcg acgctggggt atcagggcag cctgatgcgc
     2401 catttgctgg tccagaacga ctggctggcg attgcagcgc agcgcggact gccgatgaac
     2461 ccggtcgtga acaacatcaa ctattacgac aacgctggga acgcgaacta taacgcgatg
     2521 atcgcaacgc tgacgcataa tttcgaccac ggcttccagg ccgaggcgca atacacatgg
     2581 cagaaatcgc tggatgagaa ttcaggaccg tattccgagg atccctatcc atatgacgtg
     2641 cacgcggcct acgcgcgatc ggactataac gttcccaatg cggtgaagtt gttcggactc
     2701 tggcagccca tgttcttcca cggccacaat ttcgcggaga aagtggtggg tggttggtcc
     2761 ctcagcggta tttggaactg gcacacgggc ttcccgtgga acccgacgta tacaacaacc
     2821 agcaacgtct atctggcgaa cagcccgtat tcgtcgctgc gtccggcggg aattcggaag
     2881 ggctttggga cgaatgtgag caataagagc ttcgagggaa tcgaaagcgc gaacaagaat
     2941 cccaacttcg gcggcgatgg aacaccgttt ttcccgggac ccacttacac gccgggcgga
     3001 agtttccctg cgtcgagtcc gggaccgcct ccgggaattc agcgcaacag tctgaatggt
     3061 ccgcactaca gcgatctgga cgccagcctg accaaggcct tcgggttgcc gaataacaag
     3121 gtgctgggcg agaacgcgcg tgtcgaggtg cgggccgatg cgtacaacct cttcaaccaa
     3181 accaatctga acccgacgtc gatcgacggc aaccttggct ccgttggtcc cgacgggagc
     3241 ataacttcag tgaacggcga tttcggcatt gcgcgcggcg ctctcggtag ccgcacagtg
     3301 cagatgcagg cacggtttag tttctaacga cagcgaagaa ccgttagcta aggctggtga
     3361 gtcagtcaac agggggatgc caacggcgtc ctcctgtttc tttttgggcg cgggatgcgg
     3421 tagttgatgt cgcgtggagg ataattgtcc ggggagacgc gatgaaacga ttgagttcgg
     3481 ttttggtgac ggcggctctg gtggctgcga cgtggggcgg tgcggttgcg caggagtcga
     3541 gcggtgcgtc agcaacgatc gtggtcgatg cgaaggcgaa gggc
//
LOCUS       DASZYN010000316         2317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_269469, whole genome shotgun sequence.
ACCESSION   DASZYN010000316 DASZYN010000000
VERSION     DASZYN010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269469"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VHE33_08160"
     CDS             complement(<1..692)
                     /locus_tag="VHE33_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVT97467.1"
                     /translation="MRTRREFLMGSGLATAAWLTQGTLLAQAAGQNPGAAPNAATAQT
                     PATAVSTNPAVQPNPDLAQQLRTAAQTEKIKTTKLTDTIFLLQGVGGNVVCQLGPDGK
                     LVIDSQISTGTPQLLDALGKLDEHKLKLLINTHWHFDHTDGNAALHLAGAFIVAHENT
                     RSRLLKPQTMVTYAAQFPAAPDAGLPQETFAESQALWVNNDHLELVHTPEAHTDSDVF
                     IHFSRGNVIHTG"
     gene            complement(737..1066)
                     /locus_tag="VHE33_08165"
     CDS             complement(737..1066)
                     /locus_tag="VHE33_08165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HVT97468.1"
                     /translation="MLNPLKLQEMLGQAKQMQEQIQQKLAASVVEASSGGGAVTVKMN
                     GKKEVLKVSIDPAAVSSLTGANPDVEMLEDLVAAAFNEAGRRADELIKSNVQGMLGGL
                     NLPPGLL"
     gene            complement(1193..>2317)
                     /locus_tag="VHE33_08170"
     CDS             complement(1193..>2317)
                     /locus_tag="VHE33_08170"
                     /inference="COORDINATES: protein motif:HMM:NF023591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97469.1"
                     /translation="TLLNAGNSPSALARQFVRYLRNTLMAKVGGENTDLLQISADERA
                     RAMRSAMLFGEEDLTRFLQVMLRTFDELNYRQEQRFHLELGLVKLVHLQRLLPVEEIL
                     SGSTGSQVPGPRSQGVGQRTDRSALSAASSVPVTGQVRAAAPTAPRPSPFESDRDRKV
                     RGESATSSEPPRNAELNREPAARALSPAESVARASVEAKPSYSVAAPVGEAKPPVAVG
                     VAEPPGESTDGALALAASAGAAEAAPDLDRIREAVVAALADAGHETAANLIHQGNWTQ
                     QGNAIQVELAVRKTMLALTVNSEAEAVVRKTMKAMGATQKIAFVPGENGNGSKGPGRE
                     GPAPLAGSVQSAAIENPLVKKTQELFKADIRSVLDLRDKG"
BASE COUNT          360 a          827 c          651 g          479 t
ORIGIN      
        1 tcgccggtgt ggatcacatt cccgcggctg aaatggataa acacatccga gtcggtgtgc
       61 gcctcgggcg tatgcaccag ctccagatga tcgttgttga cccaaagcgc ctgcgactcg
      121 gcaaaggtct cctgcgggag ccccgcatcc ggagcggccg gaaactgcgc cgcgtacgtc
      181 accatggtct gcggcttcag caagcgcgag cgcgtatttt catgcgccac gatgaacgca
      241 cccgccagat gcagcgctgc gtttccatcc gtgtgatcga aatgccagtg ggtattgatc
      301 agcagcttca gcttgtgctc gtccagtttg cccagcgcat ccagcagctg cggggtgccg
      361 gtcgagatct gcgagtcgat gaccagcttc ccgtccggcc ccagctggca aaccacgttg
      421 ccgccgacgc cctgcaggag gaagatcgtg tcggtcagct tcgtggtttt gatcttttcg
      481 gtttgtgccg ccgtgcgcag ctgctgcgcc aggtcaggat tcggctggac cgccggattc
      541 gtgctcactg cagttgccgg agtctgcgcc gtcgccgcgt tcggcgccgc gcccgggttc
      601 tgccccgcgg cttgtgccag caaggttccc tgcgtcagcc aggcggccgt cgccaggccg
      661 gaccccatca gaaattcccg gcgcgtcctc atacacccat tagaaaccga aactccgcta
      721 cgcgccctgg acacgcctac aacagccccg gtggaagatt cagtccgccc agcatgccct
      781 gcacgttcga cttgatcagc tcgtccgccc gccggcccgc ttcgttgaac gccgcggcga
      841 ccagatcctc cagcatctcc acatccggat ttgcgccggt caggctgctc accgcagcgg
      901 ggtcgatgct gaccttcagg acctctttct tgccgttcat cttcacggtc acagcgccgc
      961 cccccgacga cgcctccacc acggatgcgg ccagcttctg ctggatctgc tcctgcatct
     1021 gtttggcctg gcccagcatt tcctgaagtt tgagaggatt cagcatcgat tcgtcttctt
     1081 actctttctg atgtagtttt cagtgctcgc ggccccttgg ccccgactgc tgagccctga
     1141 gccctggggt ctgagtccta agccctgagc cttcggatct gagccctgcg atctatccct
     1201 tgtcccgcaa atccagcacg ctgcgaatat ctgccttgaa cagctcctga gtcttcttta
     1261 ccagcggatt ctcgatcgcc gccgactgca cgcttcccgc caggggcgca ggtccttctc
     1321 ggcccggccc tttgctccca ttcccattct cgcccggcac gaacgcgatt ttctgcgttg
     1381 cgcccatggc cttcattgtt ttcctgacga ccgcctcggc ctcactgttt accgtgaggg
     1441 ccagcatcgt cttgcggacc gccagctcca cctggatcgc gttcccctgc tgcgtccagt
     1501 tgccctgatg gatgaggttt gccgccgtct cgtgtcccgc gtcggccagc gctgcaacga
     1561 ccgcctcgcg gatacgatcc aaatccggcg cagcctccgc cgctccggcc gaggcagcca
     1621 aagccagcgc tccatccgtc gattctcccg gtggctccgc cacaccaacc gccaccggag
     1681 gcttcgcttc tccaaccggt gccgctacag aataagacgg tttcgcctcc acagaagctc
     1741 gcgccacact ctcggccggc gacagcgctc tggcggccgg ttctcgattc agctcggcat
     1801 ttcgtggagg ctctgagctt gtggcactct cgcctctgac cttacggtcc cgatccgact
     1861 caaacggcga tggccgcggc gcagtcggcg ccgccgcgcg tacctgtccc gtcaccggaa
     1921 cgctgctggc tgcgctcagt gcgctgcggt ctgtgcgctg acctacgccc tgggacctgg
     1981 gacctggaac ctgcgacccc gttgagccac tgaggatttc ttccaccggc agcagtcgtt
     2041 gcagatgcac cagcttcacc agccccagct ccagatggaa ccgctgctcc tggcgataat
     2101 tcagttcgtc gaacgtccgc agcatcacct gcaggaagcg cgtcagatcc tcttcgccga
     2161 acagcatggc ggaacgcatc gcacgcgcgc gctcgtcggc tgaaatctgc agcaggtcgg
     2221 tgttctcgcc gcccaccttc gccatcagcg tgttgcgcag ataccggaca aactgccggg
     2281 cgagcgccga cgggctgttg cccgcattga gcagcgt
//
LOCUS       DASZYN010000317         1562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_721570, whole genome shotgun sequence.
ACCESSION   DASZYN010000317 DASZYN010000000
VERSION     DASZYN010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721570"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VHE33_08175"
     CDS             <1..542
                     /locus_tag="VHE33_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT97470.1"
                     /translation="PLGIWLTQKPRWARPGIGVANVLQTIPSLAMFGCLLPLPWLGER
                     ASTVAIIALTAYALLPILRNTYAGIRGIDPTLVSVAEAMGLTTRQRLLKVQLPLAASF
                     ILAGLRTATVTCVGIATIAAAVGAGGLGELIFRGVASVNNELILAGAIPAAVLALACD
                     GALGILERWFRVPGQGKAA"
     gene            539..1420
                     /locus_tag="VHE33_08180"
     CDS             539..1420
                     /locus_tag="VHE33_08180"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HVT97471.1"
                     /translation="MSRAVAVAAAILCALMTGCSPPRPDHPVVGAKNFTEQVVLGELL
                     AQEIEAKSDLEVERRFYLAGSYICNQAMKAGRIDAYVEYTGTALTAILKQPVDRDPQR
                     VLGTVRNLYQSRYQITVGPPLGFENTFAMVIRGDDAQKLHLTTLSEAAKYTPQWRLGV
                     GYEFEQRPDGLPGLESDYGLMFAGDPRVMDLGLLYRALQAHQVDMIAANSTDAQIQTL
                     GLTVLQDDKRYFPPYQAVPLVRDQALQQWPIIGDAMRALAGKVTAHDMQAMNEAVDGE
                     HRDPADVVRDFRKSKGL"
BASE COUNT          268 a          479 c          525 g          290 t
ORIGIN      
        1 ttccgctggg catctggctc acgcagaagc cgcgatgggc gaggccgggg attggggtcg
       61 ccaatgtgct gcagacgatt ccgtcgctgg cgatgttcgg ctgtctgctg cctctgccct
      121 ggctcggtga gcgtgccagc accgtggcca tcatcgctct gaccgcctac gccctgctgc
      181 cgattttaag aaacacatac gccggaattc gcgggatcga tccgaccctg gtctccgtgg
      241 cggaggccat gggcctgaca acccggcagc gcctgctcaa agtccagttg ccgctggcgg
      301 cctccttcat cctggcggga ctgcgaaccg cgaccgtgac ctgcgtgggc attgcgacca
      361 ttgccgctgc ggtcggcgca ggaggcctgg gggaactgat cttccgcggc gtggcatcgg
      421 tgaacaatga actgatcctg gcgggggcga tcccggccgc ggtgctggcc ctggcctgcg
      481 acggggccct gggcatcctg gagcgttggt tccgtgtgcc cggccaggga aaggcagcat
      541 gagccgggct gtcgccgttg ctgcggcgat cctgtgcgcg ctgatgacag gttgttcgcc
      601 gccgcggccc gatcatccgg tcgtcggagc gaaaaatttc accgagcagg tcgtgctggg
      661 cgagctgctc gcccaggaga ttgaggccaa atccgatctc gaagtggagc gccgcttcta
      721 tctggccggc agctacatct gcaatcaggc tatgaaggcc ggccggatcg atgcctacgt
      781 ggaatacacg ggaacggccc tgaccgcgat cctgaagcag cccgtggatc gcgatccgca
      841 gcgggtgctg gggacggtgc ggaatctgta tcagtccagg tatcaaatta ccgtgggtcc
      901 gccgctgggt tttgaaaaca cgtttgccat ggtgattcgc ggcgacgatg cgcagaagct
      961 gcacctcacc acgctgagcg aggcggcaaa gtacacgccg cagtggcgcc tgggcgtggg
     1021 gtacgaattc gagcagcggc cggatggtct gcccgggctt gagtcggact acggcctgat
     1081 gttcgccggc gatccgcggg tcatggacct cggcctgctc taccgcgcgc tccaggcgca
     1141 tcaggtggac atgatcgcgg cgaattcaac cgatgcacag attcagaccc tcgggctgac
     1201 cgtgctgcag gacgataagc gctatttccc gccctaccag gccgtgcccc tggtgcggga
     1261 tcaggcgctg cagcagtggc cgatcatcgg cgatgccatg cgagccctcg ccgggaaagt
     1321 caccgcccac gatatgcagg cgatgaacga ggcggtggac ggcgaacatc gcgacccggc
     1381 cgatgtggtg cgggatttcc ggaagagcaa gggtctctag tgcggcgtcc cgcaaattca
     1441 ttcaacaacc gatttggttt ttgtatcagg gcacgacttc agtcgtgccg taagcgacgc
     1501 tacaaccacg tgggcttcag cccctgaggc ctgcccgtcg gaagcctgat tgttcagtca
     1561 ac
//
LOCUS       DASZYN010000318         8538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1234495, whole genome shotgun sequence.
ACCESSION   DASZYN010000318 DASZYN010000000
VERSION     DASZYN010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8538
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234495"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..379
                     /locus_tag="VHE33_08185"
     tRNA            304..379
                     /locus_tag="VHE33_08185"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:337..339,aa:Lys,seq:ttt)
     gene            complement(534..1172)
                     /locus_tag="VHE33_08190"
     CDS             complement(534..1172)
                     /locus_tag="VHE33_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97472.1"
                     /translation="MNAFHDAFAPVSWSNWALVLVGVVAACLAWRILRRIEDQAKSGK
                     EAATAALQNAQAVMNGERAWILAELSCEGDRVQTSEIQAELPGEEGPCETTVVSNLWL
                     TCRNHGKTPGWIDEIRAQVDIVDANSVRLDPVLRKGSLGLMGPVAADDEVSTGMELRC
                     KGRRQRDDFLSVFVVISYRDIFGSTHKTTLGYTIEKGQLLLLRAAKQRNRNT"
     gene            complement(1352..1849)
                     /locus_tag="VHE33_08195"
     CDS             complement(1352..1849)
                     /locus_tag="VHE33_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT97473.1"
                     /translation="MHLTLQMATARDVSELVLFRTAVNRNLAHRFGEGFWVGRPTERG
                     ALAAMKRGSVFLGRFRGRIVASLTLSTRKPWAIDAQYFHRSVRPLYLTSMAVDPRMQG
                     KGVGRRCLEQAQRIAREWPADAIRLDAWNAEAGAGEFYRKCGYREVGRALYRKTSLIY
                     FELLL"
     gene            2299..3864
                     /locus_tag="VHE33_08200"
     CDS             2299..3864
                     /locus_tag="VHE33_08200"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase operon protein YhjQ/BcsQ"
                     /protein_id="HVT97474.1"
                     /translation="MKPDENARNAETTEQADDVATLYSWANLHGAKYRDFSASRQEMR
                     AQMRQRTLSDRARIAREEARNRAPALGEGPRWEDLLPDSDREAAAGAAAPPRRENFFE
                     PRVEKAAEPERRPTPLPPLPETSVPTRHGLGDVPYFRDADFREAAPDPHPVERIGEGE
                     GGEVRPAWLTEITPPPAPAFSSLPPAAPLPPAPPVPAAAPPAQAAHPFQTPHAFAAQP
                     LPQHQPAAPAVPPPSIEVPESDSLTHSRERVASRWFALRGVFGRNPEELQPAHAQGQV
                     PVLTFFSLAGGVGKTSLAAAIGRALAARGERVLLTDTCSFGVLPFYFGAREIKPGVVR
                     TFSGGTSDPPIRVLTLEAERYDADPDLLRREMTRGTHDVSRVLIDVATGSAPMLRQAA
                     RLSPVIVVPVVPDMASVVTLPVLEGFFRNQEGLHGKPLQAWYVLNQFDPSSPLHLDVR
                     EVLRQQLGERLLPFAVHRSPAMSEALAEGMTVIDYAPSSPASEDIMSMVTWIRNTSVA
                     AGTGQRGVRWSER"
     gene            3861..8372
                     /gene="bcsA"
                     /locus_tag="VHE33_08205"
     CDS             3861..8372
                     /gene="bcsA"
                     /locus_tag="VHE33_08205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016759 - cellulose synthase activity
                     [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HVT97475.1"
                     /translation="MTQDELWEGFETGRGPFFKLLRLVLIAFGLVALFFFGSLALSWP
                     QQAVLGLLMVLLCVWLSRVSDSYLITLILMMLSMFATFRYGWWRVSSIFAFFQDPGTR
                     WGPVDVFFIVLLLGAEAYAFGILYLGFLQTVWPLRRAPVALPDDPDDWPEIDLLIPTY
                     NEPLSVVRYTALASLNIDWPAEKLHVYILDDGNREEFREFAVEAGIGYMTRGDNSQAK
                     AGNINNALKSLSAPYVAIFDSDHVPTRSFLQMTIGWFLKDPNLAMLQTPHHFYSPDPF
                     ERNLGQFRIIPNEGELFYGIVQDGNDFWNAAFFCGSCAVLRREALDEIGGIAAETVTE
                     DAHTCLRLQIHGWNTAYINIPQAAGLATERLSAHVKQRIRWARGMIQILRIENPLFAP
                     NLSWPQRLCYFNAMTHFLYALPRLIFLTAPLIYLIFGFTNVPGYWIAILAYALPHLTL
                     SSVTNSRIQGYHRHSFWNEIYETVLSPYILLPTLLALFNPKAGKFNVTAKGGLVTRTF
                     FDARIAQPFIVLLAFNALGLLMVIPRFMHIPGLGWLWDGTHPGTIITNALWTCFNIVI
                     LSVCVAVAREARQTRQHVRINFAAPVRVEVADGRIVPGQTIDVSSGGLAMELLEGMHA
                     NHGDALKVIFPLRTGDAELPARVVGLANSVLRLQFDPLTVAEEEMLTMVLFSRADSWL
                     GWGESREVDQPLKSLARIIRIAMRGLGMSISAMIPRRRNKQEKRQQMAAESLVLLITL
                     GLSAFCAYARAQTSAPAPQSAQPVTTAPAQTPPTATPQPPAPAAIDQKNASAPASTAP
                     PAAGTFSTRTNLFDLGTTNTVNLHGADAYDTLYFAIPQNQVVKQASMHLYYHFSPSLI
                     PAQSHLKVILNGTTFATLPVPQTPNNGAVIESDVVVPPDLLVRNNQLTFELIAHYTNT
                     CEDPTNSALWAHIDPNTSLALSGVLLPLANDLKLLPLPFFDSQLAQPPVIPIVFGSAP
                     STKGLEAAGIVTSWFGMLGDYRSVHFPVSTGNLPTGNVVLIAESANALPPGLNLDPPL
                     SPTVAIRTNPVDPYGKVLIVTGNNADQTLVAAQALATGWPGLEGATASVNQLNLPVKS
                     SPDDAPRWRVANDQGRVPLWTGPAQALQGDGNEPLIAYYKVPPDIFFRDAQQNVKLHL
                     DYRYNAVPIGPISSLQVESNDAYLGSIPLFSGKEVSRQSAVNIAIPVVNLRPFSNSMR
                     FRFTFQALKGGSCNTTIPGSQMGAILRSSYIDIKGLPHWASMPNLELFSNAGFPFTRY
                     ADLSQTTVVLPEEPSAKEIETYLTMMGHFGAETGYPTLRVTVAGPDALQSGADQDFVV
                     IATGLNNGTVGRLAAGMPVTFGDNSIKVQDTRGFFAAIHNMWWRIPETDQLSTGMIGT
                     TGVPDALIEEIESPYSSGRTVVLINVKDEASYQPMLKAFLEASQSSAVSGTVALLEGP
                     RFESYRIGNNVYHVGVLPWWTALQLWFMQVPWMVDLAVLAISFVFAIWIRSWLRSRAR
                     RRLQVVESL"
BASE COUNT         1487 a         2992 c         2376 g         1683 t
ORIGIN      
        1 tgggggatat aggtgagggc taccatcgtg gcacaaagcg gcgctttgcg ctatcggtcc
       61 gcccgccctg ccctggagct ggcgcaccgg ctgaaccgag cccggctctc gaaaaaagca
      121 gaggacttac tggcgggcgc ctgtacgcga aaaccctcca cgcgaaattc ctgcaggcga
      181 aatgcggccg aacggctgca aagcctgtcc gggattcatt tcatggaccg ccgctagcgg
      241 cttcgatgtg aatgcatcgt ggggttggga ggtcccgcca acatgtaaac tggattcagt
      301 tgtgagctcg tagctcagtt ggtagagcaa cgccctttta aggcgtgggt cctgggttcg
      361 agtcccagcg ggctcaccac gcaccacgaa atcagtactg tcagaggtcc ctcgcgtcgc
      421 tcgggatttc gcctgcgggc tcccgctccg ctcacgcccg caattcggct caagttcgag
      481 tcccagcggg ctcaccacgc gccccctctc tccgccccag cgcgcccctc gtttcacgtg
      541 ttgcggttgc gctgcttcgc cgctcgcagg agcagcagct gacccttctc gatggtgtag
      601 ccgagggtgg ttttgtgggt gctcccaaag atgtccctgt agctgatgac cacaaaaacg
      661 ctcaggaaat cgtcccgctg ccggcgtcct ttacacctca gttccattcc ggtcgaaact
      721 tcgtcatcgg cggctaccgg acccatcaat cccaggcttc ctttgcgcaa aaccgggtcg
      781 agccggaccg agttcgcatc gacaatatcg acctgagcgc gaatctcgtc aatccatcca
      841 ggtgtcttgc cgtggtttcg acaggtgagc cacagattgc tcaccaccgt ggtttcgcaa
      901 ggtccttctt caccaggcag ctcggcctgg atctccgaag tttggacgcg gtccccttca
      961 caactgagct ctgccaaaat ccacgctcgt tcgccgttca tcactgcctg ggcgttctga
     1021 agggcggcag tcgcggcttc tttgccggac ttcgcctggt cctcgatccg ccgcagaatt
     1081 cgccacgcga ggcaggctgc gactaccccg accagaacca gcgcccaatt ggaccaggaa
     1141 acgggcgcga atgcgtcgtg gaatgcgttc acaatggcaa aacctatcac aacggctcgc
     1201 gtgcccgggt ggttcgatcc gtctcggcga aggacgtcca gcggtggaac cgtgcgtttg
     1261 attcgatacg gtcaaacggg ggaaggctgc ctctcacagg cagcctggca cgctctctgc
     1321 gcatccgccg ggctgctgtc gggtcagccg ttcacagcag gagttcgaag tagatcagcg
     1381 acgtcttgcg atagagcgcc cgccccacct cgcgatagcc gcacttccgg tagaactcgc
     1441 cggcaccggc ttccgcattc caggcatcca gtcgaatcgc atccgcaggc cactctctcg
     1501 cgatgcgttg ggcttgctcc aggcaccggc gacccacgcc cttgccctgc atccgcggat
     1561 cgaccgccat cgacgtcaga tacagcggtc tcacgctccg atggaagtac tgcgcgtcaa
     1621 tcgcccacgg tttcctcgtg gacaaagtca gggacgcgac gatcctgcca cgaaacctgc
     1681 cgaggaacac gctgccgcgc ttcatggccg ccagcgcgcc tctctcggtt gggcgcccta
     1741 cccaaaagcc ctcgccgaac cggtgcgcta agttccggtt gaccgctgtg cggaagagca
     1801 ccagctccga gacatcgcgc gcggttgcca tctgcagggt caggtgcatg gaactggtca
     1861 gtagggggtg aaatcgcttt cgtgtgagca caagaatagc gcgtcgccgc gcgtttggcc
     1921 gctgtcggcg ccaggtgtct ttctgcggta tcccccgggc ggaatcctcc ggagtggcac
     1981 cgtctgatgc cttttctccc cactgtcgta acggtactcc agttactgaa gaggaatggt
     2041 ttctaaccca cttaaagcgt taacttgact agacttctac ttcaagttcc gttttcattc
     2101 ggtcgccggg tttgacgcaa aagcaaacac ttccccagct aatggagaga gatgccgagt
     2161 tggggagata acgacaacag cgaagcttca tggtcgtggc aatcaggcct tagtcggatt
     2221 tctctggccg gctatccgtc tggcgccatg gctgtccatc aaactgcaac ggtcccgcaa
     2281 gctgagggtg atgccgccat gaagccggac gagaatgccc ggaacgccga gaccacagag
     2341 caggccgacg atgtcgcgac gctctactcg tgggccaacc tgcacggcgc caagtatcgc
     2401 gatttcagcg cctcgcgtca ggagatgcgc gcccagatgc ggcagcgcac tctctccgat
     2461 cgtgcgcgca tcgcacgcga ggaagccagg aaccgagctc cggctttggg cgaaggccct
     2521 cgctgggagg atctgctgcc cgacagcgat cgcgaggctg ctgccggcgc cgccgcgccc
     2581 cctcgtcgcg agaatttttt cgaaccgcgg gtcgagaagg ccgcagaacc cgagcgcagg
     2641 ccgacgccgt tgccgccgct tccggaaacc agcgtcccca cgcgccacgg cctcggggat
     2701 gttccctatt ttcgcgatgc cgactttcgc gaagccgcac cggatccaca tcctgtcgag
     2761 cgcatcggag agggcgaagg cggcgaagtg cgcccggcat ggctcacgga gattactcct
     2821 ccgcctgcac cggcattttc ctctctgcct ccggctgcgc ctttgccgcc ggctccgcct
     2881 gtgcctgccg ctgcgccgcc ggctcaggct gcgcatccgt ttcagactcc gcatgccttc
     2941 gctgctcagc cgttgcccca gcatcaaccc gcagcgccgg cggttccacc gccttccatc
     3001 gaggttcctg agagcgactc gctgacgcat tcacgtgaac gcgtggcctc gcgctggttc
     3061 gcgctgcgcg gtgttttcgg ccgcaatccg gaagaactcc agcccgccca cgcgcagggt
     3121 caggttccgg tactgacatt tttttctctc gccggcggcg tgggcaaaac cagtctcgcg
     3181 gcagcgatcg ggcgcgcatt ggctgcgcgt ggcgagcgcg tgctcctcac cgacacctgc
     3241 tccttcggcg tgctcccgtt ttacttcggt gcgcgcgaaa tcaagcccgg cgtcgttcgc
     3301 accttcagcg gcggcaccag cgatccgccc attcgcgtgc tcacccttga ggccgaacgc
     3361 tacgacgccg accccgatct gctccgtcgc gaaatgacgc gcggaaccca tgatgtcagc
     3421 cgcgtcctga tcgatgtggc caccggttcg gcgcccatgc tgcgccaggc tgcgcgcctg
     3481 tcgccggtca tcgtggttcc cgtcgttccg gacatggctt cggtggtcac gcttccggtg
     3541 ctcgaaggat tcttccgcaa tcaggaagga ctgcacggca agcctctgca ggcctggtat
     3601 gtcctgaatc agttcgatcc ctcttcgccg ctgcatctcg atgtgcgcga ggttctccgc
     3661 cagcagctcg gcgagcgcct gctgccgttc gctgtccatc gcagtcccgc gatgagcgag
     3721 gctctcgcgg aaggcatgac ggtcatcgac tacgctccca gttcgccggc ttccgaagac
     3781 atcatgagca tggtcacctg gatccggaat acttccgtcg cagcgggaac cggccaacgc
     3841 ggagtgcgct ggagcgaacg atgacccagg acgagctttg ggagggcttt gagaccgggc
     3901 gcggaccgtt cttcaagctc ctgcggctcg ttctgatcgc gttcggcctg gtcgccctgt
     3961 ttttcttcgg ctcgctggcg ctttcctggc cacagcaggc ggtcctgggc ctgctcatgg
     4021 ttctgctgtg cgtctggctc agccgggtgt cggactctta cctcatcacg ctgatcctga
     4081 tgatgctctc catgttcgcg acgttccgtt atggatggtg gcgcgtcagc agcatcttcg
     4141 ccttcttcca ggatcccggc acgcgatggg gcccggttga cgtcttcttt atcgtgctgc
     4201 tgctcggagc ggaagcctat gccttcggca tcctctatct cggcttcctg cagacggtct
     4261 ggcctttgcg ccgcgcgccc gtcgccctgc ccgacgatcc cgacgactgg cccgagatcg
     4321 atctgctcat ccccacctat aacgagcctc tcagcgtggt ccgctacact gcgctcgcct
     4381 cgctcaacat cgactggccc gccgagaaac tgcacgtcta tatcctcgac gacggcaacc
     4441 gcgaagaatt tcgcgagttc gccgtcgaag ccggcattgg atacatgacc cgcggcgaca
     4501 actctcaggc caaggccggc aacatcaaca acgctctcaa gagtctcagc gctccgtacg
     4561 tcgccatctt cgattccgac cacgtcccca cgcgcagctt cctgcagatg accatcggct
     4621 ggttcctcaa ggatcccaac cttgccatgc tgcagacccc gcatcacttc tattcgcccg
     4681 atcccttcga gcggaacctg ggccagttcc gcatcattcc caacgaaggc gagctgttct
     4741 acggcatcgt tcaggatggc aacgacttct ggaatgcggc cttcttctgc ggatcgtgcg
     4801 cggtcctgcg ccgcgaagcc ctcgacgaaa tcggcggcat cgccgctgaa accgtcaccg
     4861 aagacgcgca cacctgcctg cgcctgcaga ttcacggctg gaacactgcc tacatcaata
     4921 tcccgcaggc cgccggcctc gccaccgagc gcctcagcgc gcacgtcaag cagcgcattc
     4981 gctgggcgcg tggcatgatt cagatcctcc gcatcgaaaa cccgctcttt gcgccgaatc
     5041 tcagctggcc gcagcgcctt tgctacttca acgccatgac gcactttctc tatgcgctgc
     5101 cgcgtctcat ttttctgacc gcgccgctca tttacctcat cttcggcttc accaacgtcc
     5161 ccggctactg gatcgctatc ctcgcctacg cgctgccgca cctgacgctc tccagcgtca
     5221 ccaacagccg gatccagggc taccatcgtc attcgttctg gaacgagatt tacgagaccg
     5281 ttctttcgcc ctacatcctg ttgcccacgt tgctcgcgct cttcaatccg aaagccggga
     5341 agtttaacgt cacagccaaa ggcggccttg tcacccgcac cttttttgac gcgcgcatcg
     5401 cgcagccctt catcgtgctg ctggccttca acgcactcgg cctcctgatg gtcattccgc
     5461 gattcatgca cattcccggc ctcggctggc tgtgggacgg cacgcatccc ggcaccatca
     5521 tcaccaacgc actgtggacc tgtttcaaca tcgtcatcct cagcgtttgt gtcgccgtgg
     5581 ctcgcgaagc ccggcaaacg cgccagcatg ttcgcatcaa cttcgccgct cccgtgcggg
     5641 tcgaagttgc cgacggccgc atcgttcccg ggcaaaccat cgacgtctct tcgggcggcc
     5701 ttgccatgga gctgctggag ggcatgcacg ccaaccacgg cgatgcgctg aaagtgatct
     5761 tcccgctccg caccggcgat gccgaacttc cggcccgagt cgtcggcctc gccaacagcg
     5821 tgctgcgtct ccagttcgat ccgctcaccg tcgccgaaga agagatgctc accatggtgc
     5881 tcttctcccg cgccgactcg tggctgggct ggggcgagtc gcgcgaagtc gatcagccgc
     5941 tgaagagtct ggcccgcatc attcgcattg ctatgcgcgg cctcggcatg tccatctccg
     6001 ccatgatccc ccgccgccgc aacaagcagg aaaagcggca gcagatggcg gccgaaagtc
     6061 tggtgcttct catcacgctc ggactgagcg cattctgcgc ctacgctcgc gcgcaaacca
     6121 gcgctccggc tccacaaagc gcgcaacccg taacgacggc cccggcccaa acgcctccga
     6181 ccgcgacacc gcagccgccg gcgcccgccg cgatcgatca aaagaacgca tcggccccgg
     6241 cctcgactgc gccgcctgcg gccggaacct tcagcactcg caccaacctc ttcgatctgg
     6301 gcaccacgaa caccgtcaac ctgcacggcg cggatgccta cgacacactc tacttcgcga
     6361 tcccgcagaa ccaggtcgtg aagcaggcct cgatgcacct ctactaccac ttctcgccga
     6421 gtctcattcc ggctcagagt cacctcaagg tcatcctcaa cggcaccacc ttcgcaaccc
     6481 tgcccgttcc gcagacgccg aataatggcg cggttatcga gtccgacgtc gtggtcccgc
     6541 ccgaccttct ggttcgtaac aaccagctca ccttcgagct gatcgcgcac tacacgaaca
     6601 cctgcgaaga ccccaccaac tccgcgctct gggcgcacat cgatcccaac acgagcctcg
     6661 ccctgtccgg cgttctgctg ccgctcgcca acgatctcaa actgctgccg ctcccgttct
     6721 tcgattccca gctcgcgcag ccgccggtca tccccatcgt tttcgggtcg gcgccctcca
     6781 cgaagggcct cgaagccgcc ggcatcgtca cctcctggtt cggcatgctc ggcgactacc
     6841 gctcggtgca tttcccggtg tccaccggca acctgcccac aggcaacgtt gtgctcatcg
     6901 ctgagagtgc caacgcgctg ccgcccgggc tgaatcttga cccgccgctg tcgccgaccg
     6961 tggccattcg cacgaatcca gtcgatccgt acggcaaagt gctgatcgtc accggcaaca
     7021 atgcggatca gaccctggtc gccgcgcagg cgctcgccac cggctggcct ggtttagagg
     7081 gcgcgacggc ctcggtgaac cagctgaatc tccccgtgaa gagcagcccc gacgatgcgc
     7141 ctcgctggcg tgttgctaac gaccagggtc gcgtgcctct ctggaccgga ccggctcagg
     7201 cgctccaggg cgacggcaac gagcccctca tcgcttacta caaggttccc cccgacatct
     7261 tcttccgcga tgcgcagcag aacgtcaagc tccacctcga ctaccgctat aacgcggtac
     7321 ccatcggtcc catctccagt ctgcaggtgg aatcgaacga cgcttacctg ggatcgattc
     7381 cgcttttctc cggcaaagaa gtctcgcgcc agtcggccgt caacattgcc attcctgtcg
     7441 tcaacctgcg tcccttctcg aactccatgc gcttccgctt cacctttcag gcgctgaagg
     7501 gaggcagttg caataccacc atccccggca gccaaatggg cgccatcctt cgcagttctt
     7561 acatcgacat caaaggcctg ccgcactggg catcgatgcc gaatctggaa ctcttctcca
     7621 atgcgggctt tcccttcacc cggtacgccg acctcagcca aaccaccgtt gttcttcctg
     7681 aagagccttc ggccaaagag atcgaaacgt atctcacgat gatgggccac ttcggcgccg
     7741 aaaccggcta cccgactctg cgcgtcaccg tcgcaggccc ggatgcgctg cagtcgggcg
     7801 ccgaccagga cttcgtcgtc atcgccaccg ggctgaacaa tggcaccgtc gggcggctgg
     7861 ccgccggcat gcctgtcacc ttcggcgaca actccatcaa agtgcaggac acgcgcggtt
     7921 tcttcgcggc aatccataac atgtggtggc ggattcccga gaccgaccaa ctctcaaccg
     7981 ggatgatcgg caccaccggc gttcccgacg cgctgattga agagatcgag tcgccatact
     8041 cgagcggccg taccgtcgtc ctcatcaacg tgaaagacga agccagctat cagcccatgc
     8101 tgaaagcctt cctcgaagcc tcgcagtcca gcgccgtttc aggaaccgtc gcgcttctcg
     8161 agggcccgcg tttcgagagc taccgcatcg gcaacaacgt ttaccacgtc ggtgttttgc
     8221 cctggtggac tgcgttgcag ctctggttca tgcaggtacc gtggatggtc gacctcgctg
     8281 tgcttgcgat cagcttcgtc ttcgccatct ggattcgcag ctggctgcgc agccgcgccc
     8341 gccgccgact gcaggtcgtg gagagcctat gaggttctgt cgcaacagcc tcgcagtcct
     8401 ggctctcgcg gcattcgttt gcatcgtacc ggcccatgcg cagtcctccg ccgtgcagtc
     8461 gctgctcgaa aaggcgcagt cccaggaaca gtccggccac atcgatcttg ccgcgcaaac
     8521 ctggcagcag gttcttct
//
LOCUS       DASZYN010000319         1557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_76527, whole genome shotgun sequence.
ACCESSION   DASZYN010000319 DASZYN010000000
VERSION     DASZYN010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76527"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VHE33_08210"
     CDS             <1..213
                     /locus_tag="VHE33_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97476.1"
                     /translation="DSGFEILRFWRSTPRLKPIPVVVWTLARDTQLEICRYFGVREIV
                     SKDDDPNVLRAALASTIAGRTTTAKE"
     gene            385..>1557
                     /locus_tag="VHE33_08215"
     CDS             385..>1557
                     /locus_tag="VHE33_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97477.1"
                     /translation="MNLTTIDWLIMLLYFAFVLGIGFALKRFMKTSKDYLQAGRSLPA
                     WICGLAFISANLGAQEVIGMGASGAKYGIVTSHFYWIGAIPAMIFVGVFMMPFYYGSK
                     ARSVPEFLRLRFDEKTRAWNACSFAVMTVFSSGISMYAMARLIQTLHVFDKLFVDLGL
                     PVEWIFHFSIILSALIVLCYIFLGGLTSAIYNEVLQFFLIVAGFLPLVWVGLRNVGGW
                     SGLKASLPAAYMHSWKGMGSASTNPLGVDWFPLVAGLGFVLSFGYWCTDFLVIQTAMA
                     ANSEESARRVPLIAAIPKMFFPFLVILPGLIAIGTPMVTQHVMGSAAPVGLAATTAGS
                     ENLNAGRGLIPAKVDPVTGKVMLDTHGQPVLDYDLAIPNMLLHYFPTGILGLGLTAL"
BASE COUNT          256 a          489 c          460 g          352 t
ORIGIN      
        1 gacagtggat ttgagattct ccgcttctgg cggagcactc cgcgtctcaa gcccatcccc
       61 gtcgtcgtct ggaccctggc ccgggacacc cagcttgaaa tctgccgcta ctttggcgtt
      121 cgggagatcg tctcgaaaga cgacgatccc aatgtgctca gggcggcact cgccagcacg
      181 attgccgggc gtaccacgac ggcgaaagaa taggccccgc tctccacgac gccgcctcgc
      241 cttcatgcac gcttgcccag gctacgcacc cgttttccag cgttccccga ggcagcgtgt
      301 tgcaacctgg acttgcccaa tgcaagcgtg tacagttgcc tccgatatga ccatcgaatt
      361 ttcccgggga gggagcgaac aggcgtgaac ctgaccacga tcgactggct catcatgttg
      421 ctgtacttcg cttttgtgct gggcattggc ttcgcactca agcggttcat gaaaaccagc
      481 aaggattatc tgcaggccgg ccgctcgctg cccgcctgga tctgcggact ggctttcatc
      541 tcggccaatc tcggagccca ggaagtcatc ggcatgggcg cttccggcgc caagtatggc
      601 atcgtcacca gccacttcta ctggatcggc gccatccccg ccatgatctt cgtcggcgtc
      661 ttcatgatgc ccttctacta cgggtcgaag gcgcgctcgg tgccggagtt tctccgcctg
      721 cgtttcgacg agaagacccg tgcatggaat gcctgcagtt ttgccgtgat gacggtgttc
      781 tcgtcgggca tctcgatgta cgcgatggcg cgactgattc agactctcca cgttttcgac
      841 aagctgttcg tcgatctggg tttgccggtg gagtggatct tccatttctc gatcatcctc
      901 tcggccctga tcgtgctgtg ctacatcttc ctgggcggcc tgaccagcgc catctacaac
      961 gaggttctgc agttctttct gatcgtggcg ggtttcctgc cgctggtttg ggtggggctc
     1021 aggaacgtcg ggggctggag cgggttgaaa gcctcgctcc ccgcggcgta catgcattcc
     1081 tggaagggca tgggcagcgc cagcaccaat ccgctcggag tggactggtt tccgctggtc
     1141 gccggactgg gtttcgttct ttccttcgga tactggtgca cggatttcct ggtgattcag
     1201 acggcgatgg cggcgaattc ggaagagtcg gcacgccgag ttccgctaat cgccgccatt
     1261 ccgaagatgt tcttcccgtt tctggtcatc ctgcccggcc tgattgcgat tggcacgccc
     1321 atggtgaccc agcacgtgat gggttcagcg gcgcccgtcg gcctggcggc gaccaccgcc
     1381 ggctcggaga acctcaatgc aggccgagga ctgatcccgg caaaggtcga tccggtgacg
     1441 ggcaaggtca tgctcgacac acatggccag ccggtgctcg actatgacct cgccatcccg
     1501 aacatgttgc tgcactactt ccccaccgga attctgggcc tcggcctgac ggcgctg
//
LOCUS       DASZYN010000320         1884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_654768, whole genome shotgun sequence.
ACCESSION   DASZYN010000320 DASZYN010000000
VERSION     DASZYN010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654768"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VHE33_08220"
     CDS             complement(<1..920)
                     /locus_tag="VHE33_08220"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HVT97478.1"
                     /translation="MPSLWKPESWEQRLAELEARTGDLKEAPLRRDVRSLGQLLGRVL
                     REQAGTALYEKVEELRQSAIRRRELQGRGEAREAENLMQMAVLNISAMPVERADQLSR
                     AFAFYFELINLAETNHRKRRRLSIQITGDQKRETQRGELRGTLRAMREAGISAEKALA
                     WLRRILVVPVFTAHPTEIARRSVLMKRRRMAEFLERLDRIPVSDEELAALEEALTAEI
                     TALWQTDEVRSRKPAVTDEIRMGLDYYDVSMFETLPGLYQEVAAALKSEYGLEIELSE
                     LPLLLAFGSWIGGDRDGNPFVTPDVTRQAIL"
     gene            complement(1038..>1884)
                     /locus_tag="VHE33_08225"
     CDS             complement(1038..>1884)
                     /locus_tag="VHE33_08225"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT97479.1"
                     /translation="QSDAARTLTSEEIDALWTIGRPAEGCERLRAALDAHPGSADELR
                     TQMARALGLQGRFDEAWGELARVSENPSVTVRIRVQLESGRLRNSSGDREGSREYFMR
                     ALALAEEGGFDYFAVDAAHMLGIVSGGDASIEWNRKALDLAAHSNDERARRWKGSLLN
                     NLGWAWFDRGDFAQALGTFEEALRDQEATGNPVRIRVLRWSVARCLRALHRYEDALAI
                     QSELSQYPEEGFVSEELGELLLAMGRPEEARPYFRKAYPLLARRLGSDSRESSRLERL
                     KALSE"
BASE COUNT          357 a          709 c          485 g          333 t
ORIGIN      
        1 agaatcgcct gccgcgtcac atccggcgtc acaaacggat tgccatcgcg atcgccgccg
       61 atccacgacc cgaacgccag cagcagcggc agctccgaaa gctcgatctc gaggccgtac
      121 tcgctcttca gcgccgcagc cacttcctgg taaagccccg gcagcgtctc aaacatcgaa
      181 acatcgtagt agtccagccc catccggatt tcatccgtca ccgccggctt gcggctccgg
      241 acttcatccg tctgccacag cgccgtgatc tccgccgtca gcgcctcttc cagcgccgcc
      301 agctcttcgt cggaaaccgg aatgcgatcc agcctctcaa gaaactccgc catccgccgc
      361 cgcttcatca acaccgaccg ccgcgcaatc tccgtcggat gcgcggtgaa caccggcaca
      421 accaggatcc ggcgcagcca cgccagcgcc ttctccgccg aaatccccgc ctcccgcatc
      481 gcccgcagcg tgccgcgcag ctcgccgcgc tgcgtctctc gcttctgatc gccggtgatc
      541 tggatcgaca gccgccgccg tttgcggtgg ttcgtctccg ccagattgat cagctcgaaa
      601 taaaacgcaa acgcccggct cagctggtcc gcccgctcca ccggcatcgc gctgatgttc
      661 agcaccgcca tctgcatcag gttttcggct tcgcgtgcct cgcccctgcc ctgcagctcc
      721 cgccgccgga tcgccgactg ccgcagctct tcaaccttct cgtacagcgc cgtgcccgcc
      781 tgttcccgca gcacacgtcc cagcaactgc cccagcgacc gtacgtcgcg gcgcagagga
      841 gcttccttta ggtcgccggt gcgcgcttcc agttccgcca ggcgctgctc ccagctctca
      901 ggtttccaaa gagacggcat ctgttctgat taaaacagag agcgatgcag ttagagccct
      961 ccggttagcc tgaccccatc ttgaaagagc cacggatgca gaaccgaatc gcggatcggc
     1021 acccgcgccg aagcgcttca ttcactcagc gccttcaggc gctcaagacg cgatgactcg
     1081 cgagaatcgg aacccagacg gcgcgccagg agcggatacg ccttcctgaa ataaggcctg
     1141 gcttcctccg gccggcccat agcaagaagg agctcgccca gctcttctga gacgaagccc
     1201 tcttccgggt attggctcag ttcactctga atggccagcg catcctcgta ccgatgcagc
     1261 gcgcgcaggc atcgggcaac gctccagcgc aaaactcgaa tgcgaacagg attgcccgtt
     1321 gcttcctgat ccctgagcgc ttcctcaaac gtgccaagcg cctgggcaaa gtccccccga
     1381 tcgaaccacg cccagcccag attattcaga aggctcccct tccaccgccg cgcacgctca
     1441 tcgttcgaat gggccgccag atcgagcgcc ttccgattcc attcgatcga ggcgtctccg
     1501 ccgctcacga ttcccagcat gtgggcggcg tccacggcga aatagtcgaa ccctccttct
     1561 tccgccaggg caagcgcgcg catgaagtat tcccgcgaac cttcccgatc gccggatgaa
     1621 ttccgcagcc ggccggactc cagttgaacc cggattctga ccgtcacgga tggattctca
     1681 gacaccctgg caagctcgcc ccacgcttca tcaaacctgc cctgcagtcc cagagcgcgt
     1741 gccatctgcg tgcgcaattc atcagccgac ccaggatgag cgtcgagcgc agctctcaac
     1801 cgctcgcagc cctcagccgg ccgaccgatc gtccacaagg catcgatctc ttcgctggtc
     1861 aaagtacgag cagcatcgga ttga
//
LOCUS       DASZYN010000321         8245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_913877, whole genome shotgun sequence.
ACCESSION   DASZYN010000321 DASZYN010000000
VERSION     DASZYN010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8245
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913877"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VHE33_08230"
     CDS             <1..840
                     /locus_tag="VHE33_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HVT97480.1"
                     /translation="GEKHHMFIVGHNLVWHSQTPAWVFHDDKGNLLTRDALLARMRDH
                     IHTVVGRYKGRIQSWDVVNEALNEDGSLRQSLWYKIIGPDYIEKAFQFAHEADPAAQL
                     NYNDYNIENEAKRNGAIALVKQLQAAGIPISTVGIQEHDSLDWPTLDQLDASIDAFGK
                     LGVKVAITELDVDVLPRATRNQTSDVSVRAAQDPALNPWPNGLPESVEQQLTKRYADL
                     FGVYLKHRDVVNRVTLWGVTDADSWKNNWPVPGRTNYPLLFDRDGKPKPACDAVIRLA
                     SGK"
     gene            complement(881..1780)
                     /gene="rfbD"
                     /locus_tag="VHE33_08235"
     CDS             complement(881..1780)
                     /gene="rfbD"
                     /locus_tag="VHE33_08235"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HVT97481.1"
                     /translation="MSDGPRVLVIGGSGQLGLELQRSFAGTNPMVAVDREVVDLSNPN
                     QIRSAVATVKPDVILNSAAYTAVDRAESQPEIAAAVNARAPRVLAEEALKTGALLVHY
                     STDYVFDGTKQGPWTEADAPNPLSVYGASKLAGEEGIREVGGRYLIFRTSWVYGPHGK
                     NFLLTMLRLGRERDSLNVVDDQFGAPTTSLELARATREIVDGVLEGKFGQTSEWAGLY
                     HMTCGGETTWCGFTRAIFSRSAGLLNGRKPEVNAIPTSEYPTPAKRPRNSVLSNARLQ
                     ERFGVQLKPWETALDDVIAKIHS"
     gene            complement(1777..2322)
                     /gene="rfbC"
                     /locus_tag="VHE33_08240"
     CDS             complement(1777..2322)
                     /gene="rfbC"
                     /locus_tag="VHE33_08240"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HVT97482.1"
                     /translation="MNVEQTALPGVLVLTPKIFRDDRGAFLETWSLPMAEKAGLPNRW
                     VQDNFSISKKNVLRGIHYQVIQPQGKLVRVAYGAVLDVAVDLRRSSPAFGKHVAVELS
                     GENGRMMWIPEGFGHAFLALSEEAGFAYKVTDSYHPAGERTIRWDDPELAIPWPLEAA
                     CVIVSDKDGKGASLREAEVFP"
     gene            complement(2458..3933)
                     /gene="hldE"
                     /locus_tag="VHE33_08245"
     CDS             complement(2458..3933)
                     /gene="hldE"
                     /locus_tag="VHE33_08245"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: protein motif:HMM:NF008454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HVT97483.1"
                     /translation="MIRDLHRIIQLVQGEWRGARVLVIGDLMLDRYIWGDVERISPEA
                     PVPIVRAARQTEQPGGAANVAVNIVCLGAKATLLGFRGEDADGYALDRALREAGVEPE
                     LVTTTSHPTTTKMRILAGRQQMLRLDTEKACEYEPEAYQALLEKIDALVATVDAVVLS
                     DYAKGLLTEPVCQHTIRTARKAGLPVLVDPKQQSFARYRGATTICPNLAELSAATGVP
                     AANVELMLATGQKMVSDLEVESIAATMSEKGIAVLRRDRQYLAPAAARQVFDVSGAGD
                     TVIATLALALASQLEMESAVELANVAAGIVVGKVGTVPVNRDELLISLTPQIELQAQE
                     KMLSLDALRGRVSAWRSSGQQIVFTNGCFDLLHVGHITLLETARREGDRLVVAINSDA
                     SVRKLKGDARPIVGEWERGRILAALAAVDAVVTFDDPTPIRVIETLRPDVIVKGGDYN
                     EDSVVGASEVRSWGGRVKIVPTVEGFSTTKLIARAMAPAAG"
     gene            complement(3930..4493)
                     /locus_tag="VHE33_08250"
     CDS             complement(3930..4493)
                     /locus_tag="VHE33_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HVT97484.1"
                     /translation="MLVIESLRAQITVLERIAEELTRAVMSGNKVLWCGNGGSAADSQ
                     HLAAEFVGRFRRQRRGLASIALTTDTSVLTSIGNDYGYESVFSRQVEALCVEGDVLVG
                     ISTSGNSPNVCLALEKGRELGAFNIAMTGMGGGAMAALADATLCVPSKDTARVQEAHI
                     LCGHMLCDWVDQSVCERQIAEKEKAGR"
     gene            complement(4716..5690)
                     /locus_tag="VHE33_08255"
     CDS             complement(4716..5690)
                     /locus_tag="VHE33_08255"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HVT97485.1"
                     /translation="MPRALITGITGFVGPWLAAYLRSQNIECVGIARAATDTKHPVSL
                     AGFRIHDVDLRDRVGLGKVLAAEQPEMVFHLAAISHVPTSKANPELVFDVNATGTFNL
                     LESLRQTGSQARVVLVSTGNLYGEIDSGDEGFSEGDRLQPMSPYATSKLIGEQIARSY
                     FDDFGLAVVIARPFNHTGPGQPPSFACPEFARGIAAAVTAGQATIELKTGRLDPRRDI
                     TDVRDVVRAYALLASRGVPGEVYNVCSGSMVSMGRVVETLADLAHIGVTTQLDPLRVR
                     DREIMRSGGNCSKIRRELGWSPEIRFEEMLQDLLDYWVDQSRRSGRTG"
     gene            complement(5695..6687)
                     /locus_tag="VHE33_08260"
     CDS             complement(5695..6687)
                     /locus_tag="VHE33_08260"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042351 - 'de novo' GDP-L-fucose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HVT97486.1"
                     /translation="MPSKALITGITGQDGSYLTEFLLEKGYRVSGLVRRSSSLNLDRL
                     INVVDRIELISGDLLDQNSLVAAIEQSEPDEVYNLAAQSFVATSWRQADLTGEFTALG
                     VSRLLEACRVVNPKIRFYQASSSEMFGATNPPQNESSPFHPRSPYAVAKVFGHYMTQN
                     YRESYGMFACSGILFNHESPRRGLEFVTRKVTNAVARIKLGLQTELSMGNLNARRDWG
                     FAGDYVSAMWHMLQGEGPQDYVVATGVSHSVEYLLKVAFAYQDLDYQDYVRVDPTLIR
                     PAEVDYLCGDASRARQAFGWEPRTTFEQMIAMMVDADLERNRQNPKLVHFSAGM"
     gene            complement(6758..7804)
                     /locus_tag="VHE33_08265"
     CDS             complement(6758..7804)
                     /locus_tag="VHE33_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT97487.1"
                     /translation="MYSSNSTTDPTPDARKIVILGGSGFIGTRMTKLLRDQGSAVEIG
                     DVSRSETFPGLWTECDVRREDSLSEILRGAGSIINLAAEHRDDVRPLSRYHETNVHGA
                     QQVCEAANRAKVQKIIFTSSVAVYGFQPRPVDETGPFAPFNAYGITKLEAEQVYRAWA
                     QEDPTRTLVIVRPTVVFGEGNRGNVYNLIHQIATGKFLMVGSGDNTKSIAYVGNVAAF
                     LVHTLSLGPGTHIFNYVDGPDMRTGDLVDYIRRCLGKSGRAPRVPKSIAMGAGRILDL
                     LAHLSGRNFPVSAVRVQKFCETTQFRADRVRESGFRPPYSLQDALTRTIQFECLRDLS
                     VDQKDDGLIRTASS"
BASE COUNT         1738 a         2656 c         2156 g         1695 t
ORIGIN      
        1 ggtgaaaaac accatatgtt catcgtgggc cacaatctcg tgtggcacag ccagacgccc
       61 gcctgggtct tccacgacga caagggtaat ctgctcacgc gcgacgcgct gctcgcccgg
      121 atgcgtgatc acatccacac cgtcgttgga cgctacaagg gccgcattca gtcctgggac
      181 gtcgtcaacg aagcactcaa tgaggacgga agcctccgtc aatccctctg gtacaagatc
      241 atcggccccg actacatcga gaaggccttt cagttcgcgc atgaagccga tcccgctgcc
      301 cagctgaact acaacgacta caacattgag aatgaagcca agcgtaacgg agcgattgcc
      361 ctcgtcaaac agcttcaggc cgcgggcatt cccatcagca cggtgggcat ccaggagcac
      421 gatagtctcg actggccgac actcgatcag ctcgacgcca gcattgatgc cttcggcaaa
      481 ctcggagtga aagtcgccat caccgaactc gacgtcgatg tcctgccccg agccacgcgc
      541 aaccaaactt ccgacgtttc ggtccgcgca gcgcaggatc ccgcgctcaa tccctggccc
      601 aacggcctgc ccgaatccgt cgaacaacag ctaacgaagc gctatgccga tctgttcggc
      661 gtctatctga agcaccgcga tgtagtgaat cgtgtgacgc tctggggcgt caccgacgcg
      721 gattcatgga agaacaactg gcccgttccc ggccggacca actatcctct gctcttcgat
      781 cgcgacggca aaccgaaacc ggcctgtgat gccgtcattc gattggcgag cggaaagtaa
      841 aaacgcacaa cgggcagggg ctgcatccgc atgattgaat tcaggagtga atcttcgcga
      901 tcacgtcatc cagtgcagtt tcccagggct taagctgcac accaaagcgc tcctggagtc
      961 gtgcattcga taagacggag tttcgcggcc gctttgccgg cgtcggatat tcgctggttg
     1021 gaatcgcatt gacctccggc ttgcggccat tcagcagccc ggcagatcgc gaaaagattg
     1081 ctcgggtaaa gccacaccac gtcgtttccc cgccgcaggt catgtgataa agccccgccc
     1141 attcgctcgt ctggccaaat ttgccctcga gaacgccatc gacaatctca cgcgtcgccc
     1201 gcgccagttc cagggacgtc gtcggagcac caaactgatc gtccaccacg ttcagcgaat
     1261 cgcgctcgcg tcccagccgt aacattgtca gcagaaaatt cttgccgtgc gggccataga
     1321 cccagctggt gcgaaaaatc agataccttc cgcccacctc acggatcccc tcttctccag
     1381 cgagcttgct cgcgccgtag acgctcaatg gattcggcgc gtcggcttcg gtccagggtc
     1441 cttgtttcgt cccgtcgaag acatagtcgg tcgagtagtg caccagcaga gcccccgtct
     1501 tcaaagcttc ctccgccaga acccgcggcg cgcgcgcgtt gaccgctgcc gcgatctcag
     1561 gctgcgactc ggctcgatcc acggctgtgt aggccgctga gttcagaatg acgtctggtt
     1621 tcactgtggc gacagccgat cggatctggt ttggattcga cagatccacg acttcacggt
     1681 caacggcgac catcgggttc gtgccggcaa agctgcgctg caactccagc cccagttgcc
     1741 ccgaaccacc aatgaccagc acgcgcggtc catcgctcac ggaaagacct cagcctcgcg
     1801 caggctcgcg cccttcccat ccttgtccga cacaatgaca caggctgcct ccaacggcca
     1861 gggtatcgcc agttcgggat cgtcccagcg aatcgtccgc tcgccggctg gatggtaaga
     1921 gtcggtgacc ttatacgcaa atcccgcctc ttcgctcagc gcgaggaagg catgtccgaa
     1981 gccctccggg atccacatca tcctgccgtt ctcgccactc aattccaccg ccacatgctt
     2041 cccaaacgct ggcgaactcc ttctgaggtc caccgccacg tccagtacag cgccgtacgc
     2101 cacacgcacc aattttccct gaggctgaat cacctggtag tgaatgccac gcagcacgtt
     2161 ctttttcgat atggagaagt tgtcctgaac ccagcggttc ggcaatccag ccttctccgc
     2221 catcggcaga ctccaggtct ctaaaaatgc ccctcgatcg tcgcgaaaaa tcttcggagt
     2281 caggactaag actccaggca atgctgtctg ctccacgttc attcgttctc cctggacgaa
     2341 aaaaatggct cgcgcaaagc ttttgaagct actcgcccct cgtgtgcagc ggcgattctc
     2401 gtgactggca gtgctttgcg aatttccatc tcggcttcgg tgcgcatact ccaactctca
     2461 accagcggca ggagccatcg ccctggcgat cagctttgtg gtgctgaacc cctcgacagt
     2521 gggcacaatt ttcacgcggc cgccccagga tcgaacttcc gaggctccca ccacggagtc
     2581 ttcgttgtag tcgcctccct tcacgatcac gtcgggcctc agcgtctcaa tgacgcggat
     2641 gggtgtcgga tcgtcgaacg tcacaacggc atcgaccgcg gccagagcgg cgaggatccg
     2701 cccacgctcc cattccccaa cgatcggtcg cgcatcgccc ttgagcttcc gcaccgacgc
     2761 gtcgctgtta atcgctacta caaggcgatc gccctcgcgc ctcgcagttt ccagaagcgt
     2821 gatgtgccca acatgtaaca gatcgaagca tccattcgtg aagacgattt gctgccccga
     2881 tgagcgccac gctgacactc gaccccgcag ggcatccaga gacagcatct tctcctgagc
     2941 ctgcaattca atctgcggcg tcaggctgat caggagctcg tcccggttca ctggcacggt
     3001 cccgacttta ccgaccacga tcccggcagc tacgttcgcc agctctacgg ccgattccat
     3061 ttcaagctgg ctcgccagag ctaacgccag cgtggcaatc actgtgtcgc ccgcgccgga
     3121 aacgtcgaac acctgccgcg cagctgccgg agcgagatat tggcgatccc gccgcagcac
     3181 tgcgattccc ttctcgctca tcgttgctgc aatcgattcc acttccagat cgctgaccat
     3241 cttttgcccg gtagcaagca tcagctccac gttcgcggcc ggaacgccag tcgccgcaga
     3301 aagctctgcc aggtttggac agatcgtggt cgccccgcga tagcgcgcga agctttgctg
     3361 cttgggatcc accagcaccg gcagtccagc ctttcgtgca gtccttatcg tgtgctggca
     3421 caccggctcg gtcagcaggc ccttggcata gtccgataaa accaccgcat cgaccgttgc
     3481 gaccagggcg tcgatttttt ccagcagcgc ctgatacgcc tcaggttcat attcacaggc
     3541 tttttcggtg tcgaggcgaa gcatctgctg tcgtccggcc aggatccgca tctttgtcgt
     3601 tgtgggatgg ctggttgtgg tgaccagttc cggctccact cctgcctcac gaagcgcccg
     3661 atccagtgcg tatccatcgg catcttcccc acggaatccc agcagcgtcg ctttcgcacc
     3721 caggcagaca atattcacgg cgacgttcgc ggcgccgcca ggttgctcgg tttgccgggc
     3781 agctcgcacg ataggcactg gcgcttccgg cgaaatccgt tcaacgtcgc cccagatata
     3841 gcgatccagc atcaggtccc cgatgaccag cacccgagcg ccgcgccact ccccctgaac
     3901 gagttgaata atgcggtgca gatcccgaat catcgcccgg ctttttcctt ttccgcgatc
     3961 tgccgctcac acacgctctg gtcaacccag tcgcacagca tatgtccgca aagaatgtgc
     4021 gcttcctgaa cccgagccgt gtcctttgac ggaacgcaca gcgttgcatc cgcgagcgca
     4081 gccatggctc cgccacccat gcccgtcatc gcaatattga aggctcccag ttcgcggccc
     4141 ttctccagcg ccaggcagac gttcgggctg ttgcccgatg tcgagatgcc gaccagcaca
     4201 tccccctcga cgcaaagagc ttccacctga cggctgaaga cgctttcgta accgtaatcg
     4261 ttgccgatcg acgtcagcac tgacgtgtcc gtcgtcagcg cgatcgaagc cagtccgcga
     4321 cgctggcgcc gaaacctgcc cacgaattca gctgccagat gctgtgaatc tgcagcactt
     4381 ccgccattgc cgcaccacag gaccttgttg ccgctcatta cggcacgtgt caattcttcc
     4441 gcgatgcgct ccagcaccgt tatttgcgcg cgcaggcttt cgattacgag caagtgctcc
     4501 ctgatcgcgt ggtcgaagat tgaaatcgag ttcaacgatg atggcatggc tgactcgcag
     4561 gagggcctct caactggagt gccgacgctc gcccaaaatc atcatatcgt cataccccgc
     4621 gcttaacgat cgacaaaagc gaatcgctga aagcaataca gggcacggat attcacggat
     4681 caaccaggcc attccggacg cggtccgcga tgcgattatc ccgtccggcc agacctgcgc
     4741 gactgatcaa cccagtaatc caacaaatcc tgcagcatct cctcgaaccg aatctctggc
     4801 gaccaaccca gctcccgccg aatcttcgag caattgccgc cggaacgcat gatttcacga
     4861 tccctgactc gaagcgggtc cagttgtgtt gtcaccccaa tatgggcgag atccgcgaga
     4921 gtctcaacga cgcgccccat gctgaccatc gagcccgaac aaacgttata aacctcgccg
     4981 ggcactccgc ggctggcaag aagagcgtag gcgcgaacaa catcgcgcac gtcagtgatg
     5041 tctcgacgag gatccagccg gccggttttc agctcgatag ttgcctggcc ggcagtcacc
     5101 gctgctgcaa tgccccgggc aaactcggga cacgcaaaac tcgggggctg accgggaccc
     5161 gtatgattga agggcctggc gatcaccacg gcgaggccga agtcgtcgaa gtaggaccgt
     5221 gcaatctgct cgccgatgag cttactcgtg gcatagggag acatcggctg cagacgatct
     5281 ccctcgctga agccctcgtc accggagtca atctcgccat atagattgcc ggtgctcacc
     5341 agaaccaccc gcgcctggct tccagtctgt cgcaggctct ccagcagatt gaacgtacct
     5401 gtcgcattga catcgaatac cagttccgga ttggctttgc tggtcggaac atggctgatc
     5461 gcagccaaat gaaagaccat ctccggctgc tcggccgcca gaacctttcc caggcccact
     5521 cgatcccgca gatcgacgtc gtgaatccgg aacccggcca gggagaccgg atgcttcgtg
     5581 tccgttgctg cccgtgcaat tccgacgcat tcgatatttt gactgcggag gtatgcggcc
     5641 agccacggtc ccacgaaccc ggtgatgccc gtaatgagag cgcgtggcaa ctcgttacat
     5701 tcctgcggag aaatgaacca gcttcgggtt ttgccggtta cgctccaaat cggcatccac
     5761 catcatggcg atcatctgct cgaaagtggt tcgcggttcc caaccaaaag cctgccgcgc
     5821 tcttgaagcg tcaccgcata aataatcgac ttcggcagga cgaatgagag tgggatcgac
     5881 tcgcacataa tcctgatagt ccagatcctg atacgcgaat gctactttga gcagatactc
     5941 cactgaatga ctgacgcctg tagcaacgac gtagtcctgc gggccttccc cctgcaacat
     6001 gtgccacatc gcggaaacgt agtcgcccgc aaagccccag tcgcgccggg cgttgagatt
     6061 ccccatagaa agctccgttt gcagaccgag cttaatccga gcgaccgcat tcgttacctt
     6121 ccgggttacg aattccaatc cgcgccgtgg cgactcatga ttgaacagaa ttcccgagca
     6181 ggcgaacatg ccatagctct cgcgatagtt ctgcgtcatg tagtgaccga agaccttagc
     6241 caccgcatag gggctccgcg gatggaaggg cgaagattca ttttggggtg ggttcgtcgc
     6301 accgaacatc tcgctcgagg acgcctgata gaagcggatc tttggattga cgactcgaca
     6361 cgcttccagc agccgcgaaa cgcctaaagc cgtgaactct ccggtgagat ctgcctgacg
     6421 ccacgatgtt gcaacaaagg actgtgccgc gaggttataa acttcgtccg gttcactctg
     6481 ctcgatggct gccacaagcg agttctggtc gagcaaatct ccggaaatga gctcaatccg
     6541 atcgacgaca ttgatgagtc tgtccagatt gagcgagctg cttctgcgga ccagtccgct
     6601 gactcgatat cccttctcca gaagaaactc ggtgagatag gatccatcct ggccggtaat
     6661 tcctgtaatt aaagctttgc ttggcatttt ttgaactttc agtcgtccct gtgttatctc
     6721 cgagcccgtc aatcgttaag gaacatcgtc gaccccgtca actgctcgct gtccggatga
     6781 gtccatcgtc cttttgatcg acggacaaat ctcgcaaaca ctcaaactga atcgttcgcg
     6841 tcagggcatc ctggagtgaa tacgggggac ggaaaccgga ctcgcgcacc cggtctgcgc
     6901 ggaattgcgt cgtctcacag aatttttgca ctcgcactgc gctgactgga aaattccggc
     6961 cactgagatg agccagcaaa tcgagtattc gtccagcccc catcgcgatg ctcttcggca
     7021 cacgcggcgc ccgtccggat ttccccaaac aacgtcgaat gtagtctacg agatcgccgg
     7081 tgcgcatatc cgggccgtcc acataattaa agatgtgggt gcccggaccg agtgacagcg
     7141 tatgaaccag gaatgcagcc acattgccca cataggcaat agatttagta ttgtctccgg
     7201 accctaccat cagaaatttg ccagtggcga tctggtggat aaggttgtag acgtttcctc
     7261 tgttgccctc cccgaacacc acggtcggcc gaacaataac cagagttctt gttggatctt
     7321 cctgagccca ggcacgatac acctgctctg cttccagctt cgttattccg tatgcgttaa
     7381 aaggggcaaa tggtcccgtt tcatcgacgg gtcggggttg aaaaccgtaa accgcgacgc
     7441 tgctcgtaaa gatgatcttc tgcactttcg cacgattcgc ggcctcgcac acctgctggg
     7501 cgccatgaac gttggtttcg tggtacctgg agagcggtcg gacgtcgtcg cgatgttcgg
     7561 cagccaggtt gatgattgag cctgctccgc gcaggatttc gctcagtgag tcctcacgcc
     7621 gcacatcgca ttccgtccac aaccccggaa atgtttcgct tctcgagaca tcgccgattt
     7681 ccacggcgct cccctggtcc cgcaggagct tcgtcatcct ggtaccgata aaccccgagc
     7741 ccccgaggat tacaattttg cgcgcgtcag gagtcgggtc ggtcgttgag ttgctggaat
     7801 acacgcgcta attctatcgc aggcgcgaat ctccccgacc gccacctgag acttcgggat
     7861 ccattcgctg caccgaatgt ggtgtttctg aagttctttt accttgtcat cccgaccgga
     7921 ccctgagcgt agcgaagggg aagcggaggg acctgcttct cttccgcaga aagaaatttc
     7981 gaaaacacca cgctaagcag gcaagatctc ttcaccctgt aagccgacca ttcatcggcc
     8041 aggtcatttt acggttcgct tctcagatcg ggttacgctc ccgtcccgca ccctgcaatc
     8101 gaggcatcac tgcaggcatc acaagcacga actctgagcc taaagtgcct gattccgata
     8161 aagtgagatg tcagcagcca gtcccttcct cgaagccctc aaaatggcgc cagcgaggcc
     8221 tggattagag aacagcgatc gcaca
//
LOCUS       DASZYN010000322         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_847726, whole genome shotgun sequence.
ACCESSION   DASZYN010000322 DASZYN010000000
VERSION     DASZYN010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847726"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            376..1071
                     /locus_tag="VHE33_08270"
     CDS             376..1071
                     /locus_tag="VHE33_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97488.1"
                     /translation="MGGAFAIQKHTGAWRITKDLDVLLEARSIPGALELLRRAGFETC
                     IEDPVWLAKAWRGKYFVDLISGLGNASLCVEASWIERASHEEILGVPTRVLAAEEMIA
                     SKVFVARRERFDGSDVVHLIRACGDRLDWARVLHILDRHWQLLFWSLNLFAYIYPGRT
                     ELVPERIWTELMNRFHETVKHPQRDAAFRGSLIDPLMFAIDVNEWGERNLYREYCEHH
                     PDLLVRGAEDCPK"
     gene            1068..1775
                     /locus_tag="VHE33_08275"
     CDS             1068..1775
                     /locus_tag="VHE33_08275"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVT97489.1"
                     /translation="MKVAAVADIHIKEKNREHDVRAFSRVNDMAETLVIAGDFTDHGL
                     PEEMRGCLAVLEHVRIPIIAVLGNHDHESGHQDELAGMLRVAGVHLLDGQCFEAGGIG
                     FAGTKGFCGGFSPYELMPFGEGGIKTFVEIAEREAIKLDYGLAQLHTPVKIAVTHYAP
                     IKETVIGEPEPIFPFLGSSRLERALHRHKPALALHGHAHHGTFSAEAKCGVRVCNVSL
                     PVLRERGQEHPFAVFDF"
     gene            complement(1830..>2164)
                     /locus_tag="VHE33_08280"
     CDS             complement(1830..>2164)
                     /locus_tag="VHE33_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97490.1"
                     /translation="MEKSPFSIGTIAPTPGLVDQYFNASTGEGDEGEKDVTGPWNTGN
                     GLRLVESEVDGGLDKNSEQLDSNPYDPELNSEETSPADSTPVGEFLGDAKKAKPAAKA
                     KVSKRKAS"
BASE COUNT          383 a          729 c          633 g          419 t
ORIGIN      
        1 cggcgatcca ctcgaatcgc ttccccggtg ctggcgccga tcgtttccac aaagctcatg
       61 ggcgtcaatt ttttcagggg ttccaacgca aattctagca aagcaaggtc aacgccaacg
      121 gtgacgctca tcactgcgag cctttagact tcagcaaacc gatgggccgc tccgccgaaa
      181 agaacggtcc aggcgccctg gaacccgctc tccgcgcaca acgccgctgc gagcggctgc
      241 gtcgaagcct ctatgccgga cgacgctccg ccgctgccag tcacttcatc gacggaaccg
      301 gaatgggccg tggaggcccg gtcctgctac cgcgatgtca tcgcggctct ccacgagggc
      361 catgtcgagt gcgccgtggg cggcgccttc gccatccaga aacatactgg cgcatggcga
      421 atcaccaaag atctggatgt cctgctcgag gcgcggtcca tacccggcgc ccttgagctg
      481 ctgcgccgag ccggttttga aacctgcatc gaggacccgg tgtggctggc caaagcctgg
      541 cgcggcaaat acttcgtcga tctgatctcg ggcctcggca acgcgagtct gtgtgtggag
      601 gccagctgga tcgaacgcgc ctcccatgaa gaaattcttg gcgtacccac ccgagttctc
      661 gccgcggaag agatgatcgc ctctaaagtc ttcgtcgccc ggcgcgaacg cttcgacggg
      721 tcggacgtcg tgcacctcat ccgtgcctgc ggcgaccggc tcgactgggc gcgggtgctg
      781 cacatcctcg accggcactg gcagctgctc ttctggtccc tcaatctgtt tgcctatatc
      841 tatccaggtc gcacggagct ggtgcccgag cgcatctgga ccgagcttat gaatcgcttc
      901 cacgaaacgg tgaagcatcc ccagcgcgac gctgcattcc gcggctcgct gatcgatccc
      961 ctcatgtttg cgatcgacgt caacgagtgg ggcgaacgca acctctatcg ggaatactgt
     1021 gagcaccacc ccgatcttct ggtgcggggg gcggaggact gtccgaaatg aaggtcgcag
     1081 ccgtcgccga tatccatatc aaggagaaaa accgcgagca cgacgtccgc gccttctcgc
     1141 gcgtcaacga catggccgag accctggtca tcgcggggga tttcaccgat cacggcctgc
     1201 ctgaagaaat gcgcgggtgc ctcgccgtgc tcgaacacgt tcggattccg atcatcgccg
     1261 ttctcgggaa ccacgaccac gagagcggcc accaggacga gctagccggc atgctgcgcg
     1321 tcgccggcgt ccatctgctg gatggccagt gcttcgaagc cgggggcatc gggttcgcgg
     1381 gcaccaaagg cttctgcggc ggcttttctc cctatgagct gatgcctttt ggcgagggcg
     1441 gcatcaagac cttcgtggag atcgccgagc gcgaggccat caagctcgac tacggactcg
     1501 cccagctcca tacaccggtg aagatcgcgg tcacccacta cgcgccgatc aaagaaaccg
     1561 tcatcggcga gccggagccg atctttccct tcctcggaag ctcacggctg gaacgtgctc
     1621 tccatcgcca taagcctgcg ctcgccctgc acggtcacgc tcatcacgga actttctcgg
     1681 cggaggccaa atgtggcgtg cgcgtctgca atgtgtcgct gcccgttctg cgcgaacgcg
     1741 gccaggaaca cccttttgcg gtgttcgact tctgagccgg cacggacgct gacaggaggc
     1801 gcccctgagg agcgcctccc gcgtcgtcct tacgaggcct tccgcttcga caccttcgcc
     1861 ttcgcggccg gcttcgcctt cttcgcatct cccagaaatt cgcccaccgg cgtcgagtcg
     1921 gccggcgagg tttcttcgga gttgagttcg gggtcgtacg gattcgagtc cagctgctca
     1981 ctgttcttat cgaggccgcc gtccacctcc gattccacca gacgcagtcc gttccctgtg
     2041 ttccacggac ccgtcacatc cttctcaccc tcatcgcctt cgcccgtcga agcgttgaag
     2101 tactggtcga ccagtcccgg cgtcggcgca atcgtcccga tcgagaacgg ggacttctcc
     2161 atgc
//
LOCUS       DASZYN010000323         1750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_781266, whole genome shotgun sequence.
ACCESSION   DASZYN010000323 DASZYN010000000
VERSION     DASZYN010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781266"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1418)
                     /locus_tag="VHE33_08285"
     CDS             complement(132..1418)
                     /locus_tag="VHE33_08285"
                     /inference="COORDINATES: protein motif:HMM:NF033540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HVT97491.1"
                     /translation="MTEQEIAGLSPAFAAYLGRYRGCFLQKRTMGHFDTYCRGLLSDL
                     PRKSVEPIALEAGTAVRTLQEFLVTARWDHEQARDQLQQNLAAAVGAVRADELGTIGV
                     IDETSCQKWGDHTPGVQRQYLGCVGKVDNGIVTVHVAVAKGRFQALLDADLYLPKVWD
                     EDRARCEAAGIPAHVRYRPKWHIALDELIRLERNGLTFDWLVFDEGYGAAVPFLWILN
                     LVEQRFVAEVPVNFVVRARVGGKVQRANERLTVADAKAGQRYRLAHRTVPDSVWRASS
                     ATVWVAGRQHVLIVAINEATAEVKYFLTNATGASVAHVLKVAFRRWTVEHAFRLGKQE
                     AGLMHYEGRNYTGLMRHLILALLVLGFVATHTERLRGEKSKAHGGTSVPGPEPSMRDR
                     VSPSSRHFGETAHEQSHLLPPEAKRAGDEISQEAAA"
     gene            1490..>1750
                     /locus_tag="VHE33_08290"
     CDS             1490..>1750
                     /locus_tag="VHE33_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97492.1"
                     /translation="MSKGLIVATVALLVVSAAAAQQTLTPGDNLVVEGIPPIPAALAE
                     EVGRYTEFRTAGLLSWHPTRREILISTRFADTAQVHQVKFPGG"
BASE COUNT          317 a          566 c          530 g          337 t
ORIGIN      
        1 gtacgcttcc cagggcctcg agatcgattt tagctgcgtg tgttggggcg gagatctgat
       61 attcacttcg gcaaagaaca gctggtcgtg cagacaactt aacagtacag cgctggtatc
      121 agatgataca tctatgccgc cgcttcttgt gagatttcgt cgcctgctcg tttcgcttct
      181 ggtggtaaca gatgactctg ctcgtgtgcc gtctctccga aatgccgcga cgacggcgaa
      241 acacgatcgc gcatcgatgg ttcagggccc ggcacacttg ttccgccgtg agctttggat
      301 ttttcccccc gcagtcgctc cgtgtgggta gcgacgaatc ccagcacgag cagggccagg
      361 atcaggtgtc gcatcagccc tgtgtagtta cgcccctcgt agtgcatcaa gcccgcttct
      421 tgcttgccca agcgaaacgc atgctcgacc gtccagcgcc gaaaggcgac tttcagcacg
      481 tgagcgacgg atgcccccgt cgcgttggtc aggaagtact tcacctcggc cgtcgcttca
      541 ttgattgcca cgatgagaac gtgctgccgg ccagccaccc agaccgtcgc agagctcgcc
      601 cgccacaccg agtcaggcac ggtccggtgc gccagccggt agcgttgccc cgccttggcg
      661 tctgccaccg tcagccgttc gttggcccgc tgtaccttgc cacccacacg ggcgcggacg
      721 acgaaattca ccggcacttc ggccacgaac cgctgctcga cgaggttcaa gatccacagg
      781 aaaggcacgg cggcaccgta gccctcgtcg aagacgagcc agtcaaaagt caggccattg
      841 cgttcaaggc ggatcagctc atccagcgcg atgtgccact ttggtcggta gcgcacgtgg
      901 gcgggaatgc ccgccgcctc acagcgggcg cggtcctcgt cccacacctt gggcaggtac
      961 agatcagcat ccagcagcgc ctggaaacga cccttggcca ctgccacgtg gaccgtgacg
     1021 atgccgttgt cgaccttgcc gacgcagccc aggtactgcc gttgcacgcc tggtgtgtgg
     1081 tcgccccatt tttggcagct cgtctcgtcg atcacgccga tcgtgccgag ctcatccgcc
     1141 cgcaccgccc caacggcggc cgcgagattt tgctgaagct gatcccgtgc ctgttcgtgg
     1201 tcccagcgcg cagtaacgag aaactcctgc agcgtgcgta cggcggtgcc cgcctcgagc
     1261 gcgatgggct cgaccgactt gcgcggcagg tcggacagca aaccgcggca ataagtgtcg
     1321 aagtgcccca tggttcgctt ttgcaaaaag cagccgcggt aacgtccgag atacgcggcg
     1381 aaggccgggc tgaggccggc gatttcctgt tctgtcattg ggttccctcc cgataacaga
     1441 tttatcggca tcagggaacc agcgctgtac tgctagcagg agataggtta tgtccaaagg
     1501 cttgatcgtt gcgaccgtcg ctcttctggt cgtgtccgcg gcagcagcac agcagaccct
     1561 gacgccgggc gacaacctcg tcgtcgaagg gatcccgccg atccccgcgg cgctggcgga
     1621 ggaggtcggg cggtacacgg aatttcgcac ggccggcctg ttgagctggc atccgacgcg
     1681 gcgcgagatc ctcatcagca cgcgcttcgc ggacacggcg caggtacatc aagtcaagtt
     1741 cccgggcggt
//
LOCUS       DASZYN010000324         3751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_527008, whole genome shotgun sequence.
ACCESSION   DASZYN010000324 DASZYN010000000
VERSION     DASZYN010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527008"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VHE33_08295"
     CDS             <1..385
                     /locus_tag="VHE33_08295"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HVT97493.1"
                     /translation="QTLRLPQAHLMRANFNAADLRDSDLGGSDLTWADLRSANFRNAR
                     LRGVVFDHATLADADLSRADLTGARFDGADLSNADLHGADLNGIEWRQMAAIQHANLA
                     GVRNAPNGFVAWAMGHGAVQELAAP"
     gene            564..1949
                     /locus_tag="VHE33_08300"
     CDS             564..1949
                     /locus_tag="VHE33_08300"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HVT97494.1"
                     /translation="MQRNSKRGAEKPAASSVCERLCHGTCGRSGGGMYRMLFERNPIP
                     MWVFDRKTLRFLAVNTAAIRQYGYSETEFLRMTILEIRPEETVPAVVEDMALRQRGLQ
                     KKGAWKHRRKDGSLLDAEIVCYDLVFEGAEAMLVAAYDVTDRQRAQEQARIAEEKFRA
                     IFDNAVVGIFQHSPEGRPLNINRAFAKMHGYDSPERLLAEVSDAAAQLFVEPERMAEI
                     ARAAMEEGIVRGAEVELYRRDRSRFWVMVNLRAVRDSSGAVQVFEGTAEDITDRKAAE
                     AQVKFLAYHDALTGLPNRMLFMDRLENALAGARRKPAQAAIVFLDLDRFKDVNDSLGH
                     AAGDRMLQEIAARLRQCVREEDSVARLGGDEFLILLREVSGRDEAETAAGRVLEELTR
                     TFTLQSHTLTISCSIGISLYPEDGADGETLIRKADAAMYAAKESGSNSVRFFSIEMGR
                     TMAAPLVSANG"
     gene            2059..3117
                     /locus_tag="VHE33_08305"
     CDS             2059..3117
                     /locus_tag="VHE33_08305"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HVT97495.1"
                     /translation="MKSLLLREYGRLEIAEMPQPEPAPGEVLVRVAACGICGSDVHGY
                     DGSSGRRIPPLVMGHEAAGTVAAVGAGVQGFHPGDRVTFDSTVYCGACDYCRRGEMNL
                     CDRREVVGVSCGDYRRHGAFAEFVTVPERIVYKLPDAMGFSEAAMLEAISVALHAVHL
                     SGDSKGKTALVVGAGMIGLLTMQAAKALGYARVMIADIDATRLDLARNLGADDALHLT
                     GASLVAEVQKQTGGHGADVVLEAVGRNETIAASIDAVRKGGTVVLIGNIKPEVNLPLQ
                     KVVSRQIRLQGSAASCGEYPEAMELVANGKIRIEPLITAVASLEEGPRWFERLHSGEP
                     NLMKVVLSPDAAVVEAGR"
     gene            3114..>3751
                     /locus_tag="VHE33_08310"
     CDS             3114..>3751
                     /locus_tag="VHE33_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT97496.1"
                     /translation="MSETREEKLFDLSGQVAVVTGTSRGLGQYLARALAKAGADLVIT
                     SRTKGSLAAFEAEMKALGRRVVSLELDVRDEASIRRMAEEAQAAFGQIHILVNNAGCN
                     VRKPALDVTWDDWNLVLETNLRGSFFVAQALAKGMIGHGYGRIINIGSVTSVFGYAGL
                     APYGASRGGIRQLTMSLADDWGKHGVTVNCLAPGWFRTEQNKVMYEDAGWVD"
BASE COUNT          694 a          956 c         1440 g          661 t
ORIGIN      
        1 gcagaccctg cggctgccgc aggcgcacct gatgcgcgcg aatttcaacg ctgcggattt
       61 gcgcgacagc gatctgggtg gaagcgatct gacgtgggcg gacctgcgat cggcgaattt
      121 tcgcaacgcc cggctcagag gagtcgtgtt cgatcacgcc acgctggccg acgccgatct
      181 ctccagagcc gacctgaccg gcgcacgctt cgacggtgcc gacctgagca acgccgatct
      241 gcacggggcg gacctgaacg ggattgaatg gcggcagatg gccgcaattc agcatgccaa
      301 tctcgctggg gtaaggaatg ctccgaatgg attcgtggcg tgggcgatgg ggcatggggc
      361 ggtccaggaa ctggctgccc cgtagcccag catgcatggc gcagagcgca gcagtcgact
      421 caccgtcctg acccacctgc cgatacaagt ttcagggaat tttcgcgccc gacaaccggc
      481 aatccggcgc gggttgcgtg ttcctgatcg tcggcacgca aggctggatc ctggtgaaga
      541 gccggcaatg tgtttggacc agaatgcagc gaaacagcaa acgcggcgcg gaaaagccgg
      601 cggcgagctc ggtatgcgaa cggctgtgtc acggcacatg cggccgatcc ggcggtggca
      661 tgtaccggat gctgttcgag cggaatccaa tcccgatgtg ggtgttcgac cggaagacgc
      721 tgcgctttct cgcggtgaac acggcggcga tccggcaata cggatacagc gagacggaat
      781 ttctgaggat gacgatcctg gagatccgcc cagaggagac cgtgccggcg gtcgttgagg
      841 acatggcgct gcggcagcgg gggctgcaga agaagggggc ctggaagcat cggcggaagg
      901 acgggtctct tcttgacgct gagattgtgt gttacgacct ggtgttcgaa ggcgccgaag
      961 ccatgctggt ggctgcgtac gatgtgacgg accggcaacg tgcgcaggag caggcccgca
     1021 ttgccgaaga gaagttccga gcgatctttg acaacgcggt ggttgggatt ttccagcatt
     1081 cgccggaagg gcggccgctg aatattaacc gtgctttcgc gaagatgcat gggtacgact
     1141 ccccggagcg tttgctggcc gaggtctccg atgcggcggc gcagttgttt gtcgagccgg
     1201 agcgcatggc ggagatcgca cgggctgcga tggaagaagg gatcgtgcgc ggcgcagagg
     1261 tggagctgta ccgaagggac cgcagccggt tctgggtcat ggtgaacctg cgcgccgtgc
     1321 gggactcctc cggcgcggtg caggtgtttg aggggacggc cgaggacatc acggatcgaa
     1381 aggcggccga ggcgcaggtg aagttcctgg cgtatcacga cgcgctcacg ggactgccga
     1441 accgcatgct cttcatggat cggctggaga acgcgctggc gggagcgcgg aggaagcccg
     1501 cacaggcggc aattgtgttt ctggatctgg accgattcaa ggacgtcaac gattctctgg
     1561 gacacgctgc gggagacagg atgctgcagg agatcgcagc cagactgcgg cagtgcgtgc
     1621 gcgaagagga cagtgtggcg cgactgggcg gcgatgagtt tctgatcctg ctgcgcgagg
     1681 tgagcggccg ggacgaggca gaaacggcgg cggggcgggt cctggaggag ctcacgagga
     1741 cctttaccct tcagagccat actctgacga tcagctgcag catcggcatc agcctgtatc
     1801 cggaagatgg ggccgatggg gagacgttga ttcggaaggc agacgcggcg atgtatgcgg
     1861 cgaaggagag cggcagcaac agcgtgcggt tcttttcgat cgagatgggc cgcacgatgg
     1921 cggcaccgct ggtttccgca aacggctgac attccaagcg ctacaacacg gacatcatga
     1981 aagtggatcg cgacctctgc acgcttacaa tcggagtgcg tcggcatagt gcgccggttg
     2041 tgcgaggagg tgcgttcgat gaagtctctg ctgctgcggg agtatgggcg tttggagatt
     2101 gcggagatgc cgcagcccga accggcgccg ggtgaagtgc tggtgcgcgt ggccgcgtgc
     2161 gggatctgcg ggagcgatgt gcatgggtat gacgggtctt cgggacggcg cattccgccg
     2221 ctggtgatgg ggcatgaagc ggcggggacg gtggcggcgg tgggtgctgg ggtacaggga
     2281 tttcaccccg gggatcgggt cacgttcgat tcaacggtgt attgcggagc ttgcgattat
     2341 tgccgtcgcg gcgagatgaa cctgtgcgat cggcgcgagg tggttggggt ttcgtgcggc
     2401 gattatcggc ggcatggcgc gttcgcggaa tttgtaaccg tgccggagcg gattgtttac
     2461 aagctgccgg atgcgatggg attttccgag gcagcgatgc tggaggcgat ttcggttgcg
     2521 ctgcatgccg tgcatctgag cggcgacagc aagggaaaga ccgcgctggt ggtgggcgcg
     2581 gggatgatcg ggctgctgac gatgcaggcg gcgaaggctc tgggctacgc gcgggtgatg
     2641 atcgcggaca ttgacgcgac gcgactcgac ctggcacgaa acctgggcgc ggatgacgcc
     2701 ctgcatctca ccggagcctc gctggtggcg gaggtgcaga agcagacagg cgggcacggc
     2761 gccgatgtcg tgctggaggc ggtcgggcgc aacgagacga tcgcggcatc gatcgatgcg
     2821 gtgcgcaagg gcggaacagt ggtgctgatc gggaatatca agcccgaggt gaatttgccg
     2881 ttgcagaaag tcgtgtctcg gcaaattcgg ctgcagggca gcgcggcttc ctgcggagag
     2941 tatccggagg cgatggaact ggtggcgaac ggcaagattc gaatcgagcc gctgatcacg
     3001 gcggtggcgt cgctcgaaga gggaccgcgc tggttcgagc ggctgcactc gggcgagccg
     3061 aatctgatga aggtggtgtt gtcgccggat gcggcggtgg ttgaggcggg gcgatgagcg
     3121 agaccaggga ggagaagctg ttcgatctga gcggacaggt tgcggtggtg acggggacga
     3181 gccgcgggct gggacagtat cttgcgcggg cgctggcgaa ggcgggtgca gacctggtga
     3241 tcacgagccg cacgaaaggc tcgctcgcgg cgttcgaagc cgagatgaag gcgctgggac
     3301 ggcgcgtggt gtcgctcgag ctcgacgtgc gggatgaggc cagcattcgg cggatggcgg
     3361 aggaagccca ggctgcgttt gggcagattc atatcctcgt gaacaacgcg ggatgcaatg
     3421 tgcgcaagcc ggcgctcgac gtgacgtggg acgactggaa tctggtgctg gagacgaacc
     3481 tgcgcgggag cttttttgtg gcgcaggcgc tcgcgaaggg gatgatcggg cacggatacg
     3541 gacgcatcat caacattggt tccgtgacaa gcgtgtttgg gtatgcgggt ctggctccct
     3601 atggcgcgag ccgcggcggg attcggcagc tgacgatgag cctggccgat gactggggca
     3661 agcatggcgt gaccgtgaat tgcctggcgc cgggatggtt ccgcaccgag cagaacaagg
     3721 tgatgtacga ggatgcgggg tgggtggact a
//
LOCUS       DASZYN010000325         9279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_464491, whole genome shotgun sequence.
ACCESSION   DASZYN010000325 DASZYN010000000
VERSION     DASZYN010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 9279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9279
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464491"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1751
                     /locus_tag="VHE33_08315"
     CDS             <1..1751
                     /locus_tag="VHE33_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HVT97497.1"
                     /translation="GHFLLENAAPEDIFTPEDFSEEQREIARTTAEFAEKSVLPRAAE
                     IEAKDFAVTRELMLEAGQLGLLAVDVPEAYGGLALDKVTSALVSDRIAVSASFSVSFS
                     AHTGIGTLPVIWYGTAAQKEKYLPKLATGEWIAAYALSEASSGSDAMNVRTVARLSTD
                     GKHYVLNGEKMWISNAGFADLFIVFAKIAKENAGAEQFSAFLVERNTPGLTIGAEEHK
                     LGIRGSSTCPLVLSDCAVPVENLLGEAGKGHHIAFNILNVGRYKLGAATLGGARNTLK
                     NAVKYGKQRMAFGKPISSFGLIQEKLAEMAAGIFAGEALVYRVVGAIDAALATLPADA
                     PPTEVQKRIEEYAVECSIVKVWCSEMLDRAVDHAVQIYGGYGYVEEYPAERSYRDSRI
                     NRIFEGTNEINRLIITGFLLKRAMQGRLPLLPAIAKVMDEVMAGPGTRVEQTGPLAAE
                     KALLAAAKKMALFCSGAASQKYPTTLQDQQEIMGALADMLIETLTMESAILRAEKFAG
                     KSTLAIQLTQLSASRSFCILQNAAERVLGAVAEGDMLRTQMTIYRRLAKHEPMNTVAI
                     GRSVAEEVIAAERYPL"
     gene            complement(1748..2050)
                     /locus_tag="VHE33_08320"
     CDS             complement(1748..2050)
                     /locus_tag="VHE33_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97498.1"
                     /translation="MTPNEFHASITGADPPQELSVPLAALWWDKKGDWTRAHGLVDEL
                     DTTEGMAVHAYLHRREGSLSNADYWYSRAGRLFYRSALDEEWDALVEGLLIQPDAH"
     gene            complement(2230..3021)
                     /locus_tag="VHE33_08325"
     CDS             complement(2230..3021)
                     /locus_tag="VHE33_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97499.1"
                     /translation="MLPTIRMFVLCMTAAVAVPAFAADNPWFGTWKLDPSKSHMTGDT
                     FTYSKTASGMNHFSNGPISFDFTANGIDYPVMSNATTNWVATGANQWKETDKTNGTVT
                     GTSDIKLTPDCKTMNITSTGTHPDGTAFHDEAVYVKTRSDGCLEGTWKSTKNTQSAPP
                     DFVISQGSAADAWKWEITGWKETVEGKLDGSDLAISGPTVPAGLTICFTTDGTHKLLY
                     QVKSSGTVISKGEQVLAANGKSYTDTSWTPGKENEKQIYVYNKQK"
     gene            complement(3146..4321)
                     /locus_tag="VHE33_08330"
     CDS             complement(3146..4321)
                     /locus_tag="VHE33_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HVT97500.1"
                     /translation="MSETNALEAIPPLTSLTEDETLLRDTVRQFAQQEIAPHVRAMDE
                     AQHLDEGILRQLFELGLMAIEAPMEMGGAGASFFSAVLAVEEISRVDPAVAVIVDVQN
                     TLAVNALLRWATPEQQRLYLPRMAQQTVCAYALSEAGSGSDAFSLSTQARADGDGFVL
                     NGRKLWISNAQEADLFLVFATIDPAAGYRGITAFLVERGTQGFTVGHKEDKMGIRASS
                     TCELLFDDCRVPAANVVGEPGKGYKIAIETLNEGRIGIAAQMLGLAEGAWSLAVRYSK
                     ERKQFGKAIAEFQAVQFALAEMATQIEAARALVYNAARMKAAGVPFVKEAAMAKLFTS
                     RVAEQVASQSVEIYGGYGFVRDYPMEKFYRDAKIGSIYEGTSNMQLATISKMILSAS"
     gene            4434..4853
                     /locus_tag="VHE33_08335"
     CDS             4434..4853
                     /locus_tag="VHE33_08335"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT97501.1"
                     /translation="MPRVGNLEVLFIAGFGPIVRDREESRRLYADVLGIHFKEESGGY
                     LHTEDVPGAKSFALWPLDQAAESCFGEKDWPASIPAPQAWLEFDVEDVPSATAALEAE
                     GYRLLVKNRMEPWGQTVSRLLSPEGLLVGITFTPMLR"
     gene            4902..6128
                     /gene="serB"
                     /locus_tag="VHE33_08340"
     CDS             4902..6128
                     /gene="serB"
                     /locus_tag="VHE33_08340"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HVT97502.1"
                     /translation="MSSGDPSKAVLLHFSGHDHPGLTAEITRTLARHRIGILDIGQAV
                     VHESLSLGILIEVPPEERLTALRAELTSRAHELQLHVKFSTVRADSIQHWLRGLHQQH
                     FIITVLGRSITAEHLARVTAILAAHAMNIDRIDRLSEGLSSGGPAASACVELAVSGGA
                     AQEPAMRADFLAAAQELSIDIAFQRESIFRRNRRLFAFDMDSTLIQGEVIDELAKMAG
                     VGDKVARITEAAMRGELQFDQSFTRRVALLQGLPAERVHSLLEAIPLAEGAERLIRTL
                     RMLGYKTAILSGGFTFFAQHLQQRVGIDYVHANELEIANGVVTGRVVPPIVNGERKAA
                     FLTEIAKSEDISLEQVVAVGDGANDIPMLRVAGMGIAYRAKPLVRASAPQSVSALGLD
                     GLLYLIGVRDRDLMGR"
     gene            6174..6611
                     /locus_tag="VHE33_08345"
     CDS             6174..6611
                     /locus_tag="VHE33_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97503.1"
                     /translation="MKRPVKAMGGRVSVKPVPRPAKVKARPPFPFVLEALAPLQPEVR
                     RMFSGFAVYVGDLLVCMLLDHVKHPKDNGVWVVLAEGVDPQDKSLRRDFPSLRKIAML
                     GGVIGHWLVIPLDGKDFEGEAERFCDRLLRRDPRLGRVPASRR"
     gene            complement(6630..7706)
                     /gene="mutY"
                     /locus_tag="VHE33_08350"
     CDS             complement(6630..7706)
                     /gene="mutY"
                     /locus_tag="VHE33_08350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HVT97504.1"
                     /translation="MDFRRNLLQWFDQHSRDLPWRQTGDPWAIWVSETMLQQTRVSVV
                     IDYYTRFMARFPTLQSLAEADETEVLALWSGLGYYRRARALHEAARAVVSEHAGVIPQ
                     TAAALSRLPGVGVYTAAAVASIAFSEPVAAVDGNVERVLTRYFGHEPVPGVARSGELR
                     RAAEQLVDRERPGDFNQAMMDLGATVCLPKNPMCLACPVREGCVTRGEHAVPRAKKML
                     SRKTALALLQRRQWPKAEVLLQRRPAEASQMPNMWELPQIKEGLESEDMLLLTVRHSI
                     TNTNYYVTVYGLTTNEQKLLARDTAERVWVPVLDLPELPLTGLTKKILKRLRVMPGFS
                     MSRPVVIGEEEAAVPVLDAAAVTH"
     gene            7925..8929
                     /gene="add"
                     /locus_tag="VHE33_08355"
     CDS             7925..8929
                     /gene="add"
                     /locus_tag="VHE33_08355"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HVT97505.1"
                     /translation="MDDPSFIRRLPKAELHLHLEGTVTPETLVELSRRHDGTPLDLDQ
                     ARALYAYQDFTGFLMAFKAISEHLRTAEDYELIAWRMLQRLAAQGVVHAEVYVSVGVV
                     YYWRRCAFEPLFEGMERARVRAEQELGITAYWIFDAVRHFGPEEAAKVFRKAAELRAH
                     HPSIIGIGIGGDERRTGAEPFRELYAEARDAGLRLTAHAGETTGPEAIWGALNIGAER
                     LGHALSAIHDVDLMEVLAHRQVPLEICVTSNVKTGCCVRMEDHPLRRYFDAGLMVTVN
                     SDDPALFHSDLAQEYEGAQEHFGFTQEHLRELAANSIEASFLPAERKVMLLRGIESLQ
                     "
     gene            9009..>9279
                     /locus_tag="VHE33_08360"
     CDS             9009..>9279
                     /locus_tag="VHE33_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97506.1"
                     /translation="MAPWGLRMCCQTKNKISEVAEALLSESVPESSTSTALRAIGWTS
                     VALAVTALGLYVGRELRVRYKFKRRTPSDFFSHAGEEMGAEFGMGI"
BASE COUNT         1757 a         2989 c         2772 g         1761 t
ORIGIN      
        1 gcggacactt ccttctcgaa aacgccgcgc ccgaagacat cttcaccccg gaagattttt
       61 ccgaagagca acgtgagatt gcgcgaacca cagcggaatt cgcggaaaag tccgtcctgc
      121 cgcgcgccgc cgagatcgag gccaaagact ttgcggtaac gcgtgagttg atgctcgaag
      181 cgggccaatt gggactgctc gccgtcgacg tgccggaggc gtacggcggc cttgcactcg
      241 ataaggtgac ctcggccctg gtatccgatc gaatcgctgt ctcggcaagt ttttctgtca
      301 gcttcagcgc tcacaccggc atcggcacgc tgcccgtcat ctggtacgga accgcggcgc
      361 agaaagagaa atatcttcca aagctcgcaa ccggcgagtg gatcgcggca tatgcgctga
      421 gcgaagcgtc ctcaggctcc gacgcgatga acgtgcgcac cgtggcgcgc ctttccaccg
      481 acggaaagca ctacgtcctg aatggcgaga agatgtggat ctcgaacgcg ggctttgccg
      541 atctcttcat cgtctttgcg aaaattgcga aggaaaacgc gggagccgaa cagttctccg
      601 cgttcctggt cgagcgcaat actcccggcc tgaccattgg tgcagaggaa cacaagctgg
      661 gcatccgcgg ttcgtcgacc tgcccgctcg tgctgagcga ttgcgccgtg cccgtcgaaa
      721 acctgctcgg cgaggccggc aaaggtcatc acatcgcctt caatattttg aatgttggcc
      781 gttacaaatt aggagcggcc acgctgggcg gcgcacgcaa cacactcaag aatgcggtga
      841 agtacggcaa gcagcgcatg gccttcggca agccgatcag ctccttcggt ctgatccagg
      901 aaaagcttgc cgaaatggcc gctggtatct ttgccggcga agcgctcgtc tatcgcgtcg
      961 tcggcgcgat cgatgccgcc ctggcgactc tgccagccga cgccccaccg accgaggtac
     1021 agaaacggat cgaagagtac gcagtcgagt gctcgatcgt aaaagtctgg tgctcggaaa
     1081 tgctcgaccg cgccgtcgat cacgccgtcc agatctacgg cggatacggc tacgtggagg
     1141 aatacccggc agagcgcagt tatcgcgact cgcgcatcaa ccggattttt gagggcacga
     1201 acgagatcaa ccggctcatc atcaccggct tcctgttaaa gcgggcaatg cagggccggc
     1261 tgccgctgct gcccgcaatc gcaaaagtga tggacgaagt gatggcggga cccggcacgc
     1321 gcgtcgagca gacaggcccg ctcgcggcag aaaaagcctt gctcgccgcc gccaaaaaga
     1381 tggcgctgtt ctgctcgggc gccgccagtc agaaataccc aacgactttg caggatcagc
     1441 aggagatcat gggcgcgctc gccgacatgc tgatcgaaac cctcacgatg gaatcagcca
     1501 tcctgcgcgc ggagaagttc gccggaaaga gcacgctcgc catccagctg acccaactga
     1561 gcgcgtcacg ctcgttctgc atccttcaga atgcggcgga gcgtgtcctg ggcgcggtag
     1621 ccgagggcga tatgctgcgc acgcaaatga cgatctatcg gcgcctcgcg aagcacgagc
     1681 cgatgaacac agttgccatt ggacgttccg tcgcggaaga agtgattgcg gcagaacgtt
     1741 acccgcttta gtgggcgtcc ggctgaatga gcagcccttc caccagggcg tcccactctt
     1801 catcgagcgc cgagcggtag aagaggcgcc cggccctcga ataccagtag tcagcatttg
     1861 aaagggaccc ctcacgccgg tgaaggtacg catgtacggc catgccttcg gtcgtgtcga
     1921 gctcatccac aaggccgtgg gcacgcgtcc agtcaccttt cttgtcccac cagagggcgg
     1981 ccagcggcac ggaaagctcc tggggcggat cagcaccggt gatggatgcg tgaaattcgt
     2041 tcggcgtcat cgtcgtgtgc ggacccacac ttctgtccgc tgtcccgtga gaatcaattt
     2101 tcgattgtag ccggatgcac acccaggatt gtcacccggg acagaagccg tcctgccact
     2161 gcaacggcga tgcacgcagc cgatcggagg aggccgggcc gcctcctccc gatcgtcgcg
     2221 aaaaacgcgc tacttctgtt tgttgtaaac gtagatctgc ttctcgttct ccttgcctgg
     2281 cgtccaggat gtatcggtgt agctcttccc gttcgccgcc agcacctgct cgccctttga
     2341 gatcacggtt ccgctgctct tcacctgata cagcagcttg tgcgtgccgt cggtcgtgaa
     2401 gcaaatggtc agccctgcgg gcacggtggg tccactgatc gccagatccg atccgtccag
     2461 cttaccttcg accgtttcct tccagccggt gatctcccac ttccacgcat cggctgcgga
     2521 gccctgcgag atcacaaagt cgggaggcgc gctctgcgtg ttcttcgtgc tcttccacgt
     2581 cccctcgagg cagccatcgc tcctggtctt cacgtagacc gcctcgtcgt gaaacgccgt
     2641 tccatccgga tgggttccgg tcgaggtgat gttcatcgtc ttgcagtcgg gcgtgagttt
     2701 gatgtccgat gtgccggtga ccgtgccgtt cgtcttgtcg gtttccttcc actgattcgc
     2761 cccagtggcc acccagttgg tcgtcgcatt gctcatcacc ggatagtcaa tgccattcgc
     2821 ggtgaagtcg aacgagatcg ggccattgga aaaatggttc attccactcg cggtcttgct
     2881 gtaggtaaaa gtgtcgcccg tcatgtggct cttcgacgga tcgagtttcc aggtgccgaa
     2941 ccatggattg tcggcggcaa aggcgggaac agcaaccgct gcggtcatgc aaaggacaaa
     3001 catccgaatc gtgggtagca tagggttcct ccgaatacgg cgctgaatgg ggcgcgagtg
     3061 cgtagagcgg cacagcacga ccgcaatcac ccgtgagggg aggcacgaag tgtagcaccg
     3121 cggaccgaga ggaagctcgc tgaacttacg atgcgctcaa aatcatcttt gatatggtcg
     3181 ccagctgcat gttcgacgtg ccttcgtaga tcgagccgat cttcgcgtcc cggtaaaact
     3241 tctccatcgg atagtcgcgg acaaacccat acccaccgta aatttccacc gactggctgg
     3301 cgacctgttc cgcaacgcgc gacgtaaaga gcttggccat cgcggcttct ttcacgaaag
     3361 gaactccggc cgccttcatg cgggccgcgt tgtagaccag cgcccgagcg gcttcgatct
     3421 gcgtcgccat ttcggcaagg gcaaactgca cagcctggaa ttccgcaatt gccttaccga
     3481 actgctttcg ctccttgctg tatctcaccg ccagcgacca ggcgccttcg gccagcccca
     3541 gcatctgcgc cgcgatgccg atccggcctt cgttcagcgt ttcgatcgcg atcttgtacc
     3601 ccttgcccgg ttcgccaaca acattcgcag ccggaacccg gcagtcgtcg aacagcagct
     3661 cgcaggtcga cgaagcgcga attcccatct tgtcttcttt gtggcctacc gtgaacccct
     3721 gcgtcccgcg ctccaccaga aatgccgtga tgccgcggta gccggctgcc ggatcgatgg
     3781 tggcgaagac cagaaagagg tccgcctcct gagcattcga gatccacagc ttgcgcccgt
     3841 tcagaacaaa gccatcgccg tcggcacgag cctgagtcga cagcgagaag gcgtccgacc
     3901 ccgatcccgc ctcgctgagc gcgtaagcgc agacggtttg ctgcgccatg cgcggcaaat
     3961 acaggcgctg ctgctccggc gtagcccagc gaagcaatgc gttcaccgca agcgtattct
     4021 gcacgtcgac aatgaccgcg acggcggggt cgaccctgga tatttcctcc acggcaagca
     4081 cagccgagaa aaacgaagcg cccgcgccgc ccatctccat cggagcttcg atggccatca
     4141 gaccaagctc gaaaagctgg cgcaggatgc cctcatcgag atgctgcgcc tcgtccatgg
     4201 cgcggacgtg cggcgcgatc tcctgctgcg cgaactggcg gacagtatcg cgcaggagcg
     4261 tctcgtcttc ggtcagtgac gtgagcggcg ggatggcctc aagggcgtta gtctcggaca
     4321 tggtccatag ggtacagggc gcggggtgcg gggtgcacgt caaggcccag gaggctccgg
     4381 cgtcgaacca gcaacagcaa gaagagtagg ctggctgcga ggaaggatcc accatgccgc
     4441 gtgtgggaaa tcttgaggtg ctgttcatcg ccggtttcgg tccgatcgtt cgcgaccgcg
     4501 aagagagccg ccggctctat gcggatgtcc tcggcatcca tttcaaggaa gagagtggcg
     4561 gctacctgca caccgaggac gtgcccggcg caaagtcctt cgcgctgtgg ccgctcgatc
     4621 aggcggctga gtcctgcttc ggcgaaaagg actggcctgc atcaattcct gcgccgcagg
     4681 cctggctcga gttcgatgtg gaggacgttc cgagcgcaac cgcggcgctg gaggcagagg
     4741 gatatcggct cttggtgaag aatcggatgg agccttgggg ccaaaccgtg agccgcctcc
     4801 tgtcgcccga gggtctgctg gtggggatca cgttcacgcc catgttgcgc tagctccgcg
     4861 gcccatcctc gcacccgcgg cgtctgctct atagtcggag aatgtcctct ggcgacccca
     4921 gcaaggccgt cctcctgcat ttttccgggc acgaccatcc cggtctcacg gccgagatta
     4981 cccgcaccct cgcacggcac cgcatcggca ttctcgacat cggccaggcc gtggtgcacg
     5041 agagcctctc gctcggcatt ctcatcgagg ttccgcccga ggagcggctc accgcactca
     5101 gggcggagct aaccagccgc gcccacgagc tacagctaca tgtgaagttt tccaccgtgc
     5161 gcgccgactc gatccagcac tggctccgcg gccttcatca gcagcacttc atcatcaccg
     5221 tcctcgggcg ttcgatcacc gcagagcatc tggcgcgcgt cacggccatc ctggctgctc
     5281 atgcgatgaa tatcgaccgc atcgaccgtc tgtccgaagg gctgtcctca ggcggcccgg
     5341 cagcgagcgc ctgcgtcgag ctggcggtga gtggcggcgc cgcccaggag cccgccatgc
     5401 gagccgattt cctcgccgcg gcccaggagc tctccatcga catcgccttt cagcgcgaga
     5461 gcatcttccg ccgcaaccgg cggctcttcg cgtttgacat ggactcgacc ctcatccagg
     5521 gcgaggtcat cgacgagctc gcaaaaatgg ccggcgtcgg cgacaaagtc gcccgcatca
     5581 ccgaagccgc catgcgcggc gagttgcaat tcgaccagag cttcaccagg cgtgtcgccc
     5641 tgcttcaggg acttcccgcc gagcgtgtcc actctctgct ggaagccatc ccgctggctg
     5701 aaggagccga acgcctcatc cgcaccctgc gcatgctcgg atacaagaca gcgatcctct
     5761 ccggcggctt cacattcttc gcgcagcacc tgcaacagcg cgtcggcatc gactacgtgc
     5821 atgccaacga actcgaaatc gcgaatggtg tcgtcaccgg ccgcgtggtt cccccgatcg
     5881 tcaatggcga acgcaaggcc gctttcctga ccgagatagc aaagagcgaa gacatttcgc
     5941 tcgagcaggt ggtggcggtc ggtgacggcg ccaacgacat tcccatgctc cgggtcgccg
     6001 gcatgggcat cgcctaccgg gccaagccgc tggtgcgcgc cagcgcgccg caatcggtct
     6061 cagcgctcgg cctcgatggt ttgctctatc tgatcggcgt gcgggatcgc gacctgatgg
     6121 gcagatgaaa ggcggttggc tgataatgcg gttagctggt gaggtggtca gcgatgaagc
     6181 gtccagtgaa ggcgatgggg ggtagggtct cggtcaagcc tgttccgcga ccagcaaagg
     6241 ttaaagccag gccgccgttt ccgtttgtcc tcgaagcgct cgcgccgctc cagcccgaag
     6301 tccgccgcat gttctccggc ttcgccgtct atgtgggcga tctgctggtc tgcatgctgc
     6361 tggatcacgt gaagcatccg aaagacaacg gcgtgtgggt cgtgctggcg gagggagtgg
     6421 atccgcagga caaaagcctg cgccgggatt ttccgtccct gcgcaagata gcaatgctcg
     6481 gcggtgtcat tggacactgg cttgtgatcc ccctcgatgg aaaggatttt gagggcgaag
     6541 cggagcggtt ttgcgatcgg ctgctgcgtc gcgatccacg cctcgggcgg gtgcccgcct
     6601 cgaggcgata gatcggcatt tgctgcttgc tagtgcgtta ccgcggcagc atcaagaacg
     6661 ggaaccgccg cttcttcttc cccgatcacc accggccgcg acatactaaa acccggcatc
     6721 acccgcagac gcttgaggat cttcttcgtc agcccggtta gcggcagctc cggcaaatcc
     6781 agtaccggaa cccagacccg ctcggctgta tcgcgcgcca gcagtttctg ctcgttcgtg
     6841 gtgagcccgt agaccgtcac gtaatagttg gtgttcgtga tggagtgccg caccgtgagc
     6901 aggagcatgt cctcgctctc caggccctct ttgatctgcg gcaattccca catgttcggc
     6961 atctgcgacg cctcggcagg ccgccgctgc agcagaactt ccgctttcgg ccactggcgg
     7021 cgttgcagca gagccagagc cgtcttgcgg ctcagcattt tcttcgcccg cggaactgcg
     7081 tgctcgcccc gcgttacgca tccttcgcgc accgggcagg ccaggcacat aggattcttg
     7141 ggcagacaca ccgtcgcgcc cagatccatc atggcctggt tgaagtcgcc agggcgctcg
     7201 cgatccacca actgctcggc cgcgcggcga agctcgccgc tgcgggccac tccaggcacc
     7261 ggttcgtgcc caaaatagcg ggtcagcacg cgctccacgt tcccgtcaac agccgccacc
     7321 ggctccgaaa aggcaatgct ggccaccgcg gcagcggtgt aaacacccac gccgggcaaa
     7381 cggctcagcg cggctgctgt ctgcggaatg actcccgcat gctctgaaac cacggcgcgc
     7441 gccgcttcat gcagggcgcg tgcgcggcga tagtacccca gtccgctcca cagcgccaaa
     7501 acttcggttt cgtcggcctc cgccagcgac tgcagcgtgg gaaaacgcgc catgaagcgc
     7561 gtgtaatagt cgatgaccac agagacacga gtctgctgca gcatggtctc cgaaacccag
     7621 atggcccagg ggtcgcccgt ttggcgccac ggcaaatccc gcgagtgctg atcgaaccat
     7681 tgcagcaaat tacgacgaaa gtccaaggcg tgcctcttcc ctcaaagggg attgtaggtt
     7741 tgattccagc cattttcggg ggattgggca ataccacgaa agcggatgca tcgaaaagat
     7801 tactttagag gtaagcaaca tagggcatcg gggttagccg attcgacagc agtctgcccc
     7861 gtctgccggc acgtccctaa ccgctattcg ctaaatgcta tccgctattc tgaacagttg
     7921 ccgcatggac gatccctcct tcattcgccg cctgcccaaa gccgagctgc atttgcatct
     7981 ggaaggcacg gtgactccgg aaaccctcgt cgagctcagc cgccgccacg acggcacacc
     8041 cctcgacctc gatcaggccc gagccctcta cgcctaccag gatttcaccg gctttttgat
     8101 ggccttcaag gccatcagcg agcaccttcg caccgccgag gattacgagc tgatcgcctg
     8161 gcgcatgctc cagcgcctcg ccgcccaggg cgtggtgcac gcggaggttt atgtctccgt
     8221 aggggtggtc tactactggc gtcgctgcgc gttcgagccg ctgttcgaag gcatggagcg
     8281 ggctcgggtg cgagccgagc aggagctcgg catcaccgcc tactggatct tcgatgccgt
     8341 tcgccacttc ggtcccgaag aagccgcgaa ggtctttcgc aaagcggcgg aactgcgcgc
     8401 gcaccatcct tccatcatcg gcatcggcat cggcggcgac gaacgccgta ccggagccga
     8461 gccctttcgc gaactttacg ccgaggcgcg cgatgccgga ctccggctca ccgcccatgc
     8521 cggcgaaacc accggaccgg aggccatctg gggtgccctc aacatcggtg ccgagcgcct
     8581 cggccatgcg ctgtcagcca tccacgacgt ggatctgatg gaggtgctcg cccatcgcca
     8641 ggtcccgctc gagatttgcg ttacctccaa cgtgaaaacc ggctgctgtg tccggatgga
     8701 ggatcatccc ctgcgccggt attttgacgc cgggctcatg gtgacggtca actccgacga
     8761 cccggccctt ttccacagcg atctggcgca agagtacgaa ggtgcgcagg agcacttcgg
     8821 cttcacccag gagcatctcc gcgaactggc cgcaaactcc attgaggcca gctttctccc
     8881 cgctgaacga aaagtgatgc tccttcgtgg gatcgagtcc ctccaatgag gaatggccgg
     8941 cagaatttga ccgtcctata accttagcta ccatgggcca acgtgtttag ctgcgctatg
     9001 atggacccat ggcgccttgg ggattgcgca tgtgctgtca gacaaagaac aagatcagtg
     9061 aagttgccga agccctgctt tcggagtccg ttcctgagtc ctccacctcg accgcgttgc
     9121 gcgccatcgg gtggacctcc gtggcgcttg ccgtcaccgc cctcggcctc tacgtaggcc
     9181 gcgagctccg cgtccgctac aaattcaaac gccgcacccc gtccgacttc ttctcccacg
     9241 caggcgagga gatgggcgcc gaattcggca tgggaatct
//
LOCUS       DASZYN010000326         3771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_781367, whole genome shotgun sequence.
ACCESSION   DASZYN010000326 DASZYN010000000
VERSION     DASZYN010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781367"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VHE33_08365"
     CDS             <1..546
                     /locus_tag="VHE33_08365"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HVT97507.1"
                     /translation="ATAGGVMREISLDDAAMQLAFATRVIFVPGYGLATAQAQHAVRE
                     LAELLESRGVTVKYAIHPVAGRMPGHMNVLLAEANVPYSELCEMEQINPEFPTTDVAV
                     VIGANDVVNPDARDNPKSLIAGMPILEVDRAKSVIVLKRGQGRGFSGLDNPLFFKPRT
                     TMLYGDAKSSLTHLAQAVQHV"
     gene            complement(595..1677)
                     /locus_tag="VHE33_08370"
     CDS             complement(595..1677)
                     /locus_tag="VHE33_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HVT97508.1"
                     /translation="MSYRIPMGPYHPALEEPYKLEMFCEGETVCDARLHIGFNFRGIE
                     HLAESRNYIQVIALMERVCGICSNIHTLTLCQAMEQLTGLEPPPRALYIRVIMAELER
                     LHSHILWAGIASKLMGFKTMFMSCFALREKIMDVLQAISGNRVNYAMNRIGGTNRDIS
                     DPDDILAAVNKLESEITGTVIPIFTTSATARLRCAGIGVLTHERAIACGVVGPTARAS
                     GVTQDIRQAAPYAAYPELDFHVTVESAGDVRARLFVRALEIVQSCRLLRQALTKIPSG
                     PYNTGDAKIAFAAGTAISRAEAPRGEIVYSVSWKENSRTPARVHVRTPTYANMPAVRW
                     MVQGARLADTPLIQASIDPCYSCTDR"
     gene            complement(1674..2081)
                     /locus_tag="VHE33_08375"
     CDS             complement(1674..2081)
                     /locus_tag="VHE33_08375"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HVT97509.1"
                     /translation="MTPLETITADLSITTPWSSHGGVHWLAVDASQIRRVAETMNRLA
                     ARFVTITATELPASEGFLLEYLWDLDGVLFGFPIHLAGTSLESIFDICPAVDWIEREI
                     HEGFAIDFSGRDYEPLLLRAGDKTGVNLREVPA"
     gene            complement(2078..2488)
                     /locus_tag="VHE33_08380"
     CDS             complement(2078..2488)
                     /locus_tag="VHE33_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HVT97510.1"
                     /translation="MRILTLLWTNLRAGVMTLRFPDRPPVSEGYRGLVRFDPSLCIGC
                     AMCRFRCTSRAITFEPGKTDFTWSYDPGRCTFCGRCVEGCKEHALTQERACPPIYLNA
                     GELRQSWTLPRRPPPRKPATAPDPAPAKTAGEVS"
     gene            complement(2485..3399)
                     /locus_tag="VHE33_08385"
     CDS             complement(2485..3399)
                     /locus_tag="VHE33_08385"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HVT97511.1"
                     /translation="MNLVTALVRLLIFPGLLFAVPAAWLFLWIERKSVALMQQRVGPP
                     FMQPMFDFVKLMGKNTPERSNAENLLMRIWPLIAISAAAGAVGLLPVLPSYDGFQGDL
                     ILLLALLELPSVCIVAAGFSSRSLFGEIGSAREAILTVAWNIVFLFAILSVAVSQHTF
                     RLEALARLPQSPLRYLAILAILMCLPAKLHINPFSLPNAEQEIYSGPLTEYAGPELAM
                     WELSHGLEWVASTGLVATLLIPQLSSAWIDVVAFAGLSIALVLLLSAVAAATARLAID
                     TTVRFYWRVTGVFTVLVICAAVFFRVRS"
     gene            complement(3396..>3771)
                     /locus_tag="VHE33_08390"
     CDS             complement(3396..>3771)
                     /locus_tag="VHE33_08390"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97512.1"
                     /translation="LVASLTPRYDAEQLGVRLQGSPRHADILCVTGPVTQTSVHAVET
                     VYGQVCEPKAVIAIGSCPASTNVFIDSRVLVGPLNRHIPVDVFVPGCPPRPDAIVQGI
                     VAAAEIVAKRGRMTTPEKEPPR"
BASE COUNT          797 a         1181 c         1135 g          658 t
ORIGIN      
        1 gccacggccg gcggcgtcat gcgcgaaatc tctctcgacg acgccgcgat gcagctcgcc
       61 ttcgcaacgc gcgtcatctt cgtgcccgga tacggcctcg ccaccgccca ggcccagcat
      121 gctgtccgtg aacttgccga gctcctcgaa tcccgcggcg tcaccgtcaa gtacgccatc
      181 catcccgttg cgggacgtat gcccggccac atgaacgtcc tcctcgctga agccaacgtg
      241 ccttattccg agctgtgcga aatggagcag atcaatcccg aatttcccac caccgacgtg
      301 gccgtcgtta tcggcgccaa cgatgtcgtc aatcccgacg cgcgcgacaa cccgaagtcg
      361 ctcatcgccg gcatgcccat ccttgaagtc gaccgcgcca aaagcgtgat cgtccttaag
      421 cggggccagg gccgcggctt ctccggcctc gacaacccgc tgttctttaa accccgcacc
      481 acgatgctgt acggcgacgc aaaatcttca ctcacgcacc tggcccaggc cgttcagcac
      541 gtctaggtga cttgctatgc ctcggatcgc cgtttgcctg gacggccggc accattagcg
      601 atcggtgcag gaatagcacg gatcgatcga tgcctggatg agcggcgtgt ccgccaggcg
      661 cgcgccttgc accatccagc gtacagccgg catattggca tacgtcggag tgcgtacgtg
      721 gacgcgggcg ggtgttcggg agttctcttt ccacgagacg ctgtagacga tctcgccccg
      781 cggcgcttcc gcgcggctga tggccgttcc cgcagcgaaa gcgatcttcg cgtccccggt
      841 gttgtacggc ccggacggga ttttcgttag agcctggcga agcaggcggc agctctgcac
      901 gatttccagc gcgcgaacga acagccgcgc tcgcacatcg cccgccgatt ccaccgtcac
      961 gtggaaatca agttccggat acgcggcgta gggagcggcc tggcggatat cctgggtcac
     1021 gccggaggcg cgagccgtgg gtccaacaac cccgcacgca atcgctcgtt cgtgggtcag
     1081 aactccaatt ccggcgcagc gcagccgcgc ggtagcgctg gtagtaaaaa tcgggattac
     1141 cgtgccggtg atttcgctct ccagcttgtt gactgcagcc aaaatgtcgt ccggatcgct
     1201 gatgtcgcga tttgtccctc cgatgcgatt catcgcatag ttcacgcgat tcccggagat
     1261 ggcctgcagg acgtccatga tcttttcgcg cagcgcgaaa cacgacatga acatcgtttt
     1321 gaagcccatc agtttggaag cgatcccggc ccacaggatg tgcgaatgca gccgctccag
     1381 ctctgccatg atgacgcgaa tgtagagtgc gcgcggcggc ggttcgagtc cggtgagttg
     1441 ctccatcgcc tggcaaagag tcagagtgtg aatattcgag cagatgccgc atacgcgctc
     1501 catgagcgca atcacctgga tgtagttacg gctttcggcg aggtgctcga tgccgcgaaa
     1561 gttgaacccg atatgcagac gggcatcgca taccgtctcg ccttcacaga acatctccag
     1621 cttgtaaggc tcctcaagcg ccggatggta gggacccatg ggaatgcgat agctcacgct
     1681 ggcacctccc gcagattaac gccagtcttg tcgcccgcgc gcagaagcag gggctcgtaa
     1741 tcgcgcccgc tgaagtcgat ggcgaatccc tcatggattt cgcgttcgat ccagtccacc
     1801 gcaggacaga tgtcaaagat gctctccagc gaagtgccgg cgaggtgaat cgggaagccg
     1861 aacagcaccc cgtcgaggtc ccacaaatac tcgaggagaa agccttcgct ggcgggcagc
     1921 tcggtggctg tgatggtgac aaatcgagcg gcgagccgat tcatcgtttc cgccactctg
     1981 cgaatctgcg aagcatcgac ggcgagccag tgaacgccgc catgcgacga ccagggcgtc
     2041 gtgatggaga ggtcagcggt gatggtttcc agtggtgtca tgacacttct ccggcagtct
     2101 ttgcaggagc aggatcaggg gccgttgccg gcttgcgcgg cggcggcctg cgcggcagcg
     2161 tccacgactg cctcagttct cctgcgttca gatagatggg cggacaggcc cgttcctgcg
     2221 tgagcgcatg ttccttgcag ccttccacgc agcggccgca gaacgtgcat cggcccgggt
     2281 catagctcca cgtgaaatct gtctttccgg gctcgaacgt gatcgcacgt gaggtgcaac
     2341 ggaaccggca catcgcgcag ccgatgcaaa gcgagggatc gaaccgaacc agtccgcgat
     2401 atccctcgct gaccggcgga cgatccggaa agcgcagtgt catgactccg gcgcgcagat
     2461 tcgtccacag caaggtgagg attctcatga ccgcacccgg aaaaacaccg ccgcacagat
     2521 gaccagcacc gtgaacacgc ccgtgacgcg ccagtaaaag cgaaccgttg tgtcaatcgc
     2581 aagtcgcgca gtggccgcag ccaccgcgga cagcaaaagc acaagagcga tcgacaaacc
     2641 ggcgaaggcc acaacgtcga tccatgcaga cgacaactgc gggatcagca gcgtggccac
     2701 caaaccagtg gaggcgaccc attccaaccc atgcgaaagc tcccacatgg ccagttccgg
     2761 tcccgcgtat tcggtcagcg ggcccgagta aatttcctgc tcggcgttgg gcagcgagaa
     2821 cgggttgatg tgaagtttgg cgggcaggca catcaggatg gccaaaatgg ccaggtagcg
     2881 aagcggtgac tgcggcaaac gcgccagtgc ctcgagccga aatgtgtgtt gcgagaccgc
     2941 caccgagaga atggcgaaga gaaatacgat gttccaggcc acggtgagga tcgcctcgcg
     3001 cgcagaaccg atttccccga agagagatcg cgacgaaaag cccgccgcaa cgatgcacac
     3061 cgagggcagt tccagcaatg caaggagcaa aatgagatcg ccctgaaaac cgtcgtacga
     3121 aggcagaaca ggcaggagtc caaccgcccc cgcggcggcg gaaatcgcaa tcagcggcca
     3181 gatgcgcatg agcaggtttt ctgcgttgct gcgctccggc gtgttcttgc ccatcagctt
     3241 gacgaagtcg aacatgggct gcatgaaggg tggaccgacg cgctgctgca tcaatgccac
     3301 ggatttccgc tcgatccaca gaaacagcca ggccgcaggc acagcaaaaa gaagacccgg
     3361 gaagatgaga aggcgcacga gggcggtaac aagattcatc gcggcggctc cttctccggc
     3421 gttgtcattc tgcccctttt cgcaacaatc tccgccgctg caacgatgcc ctgaacaatc
     3481 gcgtcgggcc gcggcggaca tccaggtacg aacacatcca ccgggatatg tcgattcaga
     3541 ggtccgacaa ggacgcggct gtcaataaag acattggtgg aggcggggca cgatccaatc
     3601 gcgatcactg ccttcggctc gcagacctgt ccataaacgg tctcgacggc gtggaccgac
     3661 gtttgtgtaa ccggacccgt tacacaaagg atatcggcgt ggcgtgggct gccctgcagc
     3721 cgcaccccga gctgctccgc gtcataacga ggcgtcaaac tcgcgaccag t
//
LOCUS       DASZYN010000327         4012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_914028, whole genome shotgun sequence.
ACCESSION   DASZYN010000327 DASZYN010000000
VERSION     DASZYN010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914028"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1413)
                     /locus_tag="VHE33_08395"
     CDS             complement(<1..1413)
                     /locus_tag="VHE33_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HVT97513.1"
                     /translation="MFPEPKSQLRKTMGFWDVLLFNIAAVLGPRWIAAAGHNGTSSIS
                     LWVIAALFFFVPGALVINELSSRFPEEGGLYVWAKEAFGDFHGFIAGWTYWIYTFFYF
                     PGLLLASASMAAYVLGPGGAGLAGDRAFQIWVSMGLLLVAVGMNIIGLNIGKWLQNAG
                     GVGTYVPLLILLAVGATVALRHGPGFGSATHFTLRNMLPTWNWSTVNFWSQIAFAFTG
                     LELVSAMSEEIRDPRRTLPRAVLGSGAMIAFVYIAGTFAVLALVPAPSIDPQSGVFHA
                     ITVGSVALKIGFLGILAAILVTVGNAGGVGSTVAGIARVPFVVGIDRYLPRAFGWIHP
                     RWKTPWVSILVQGGISAVILLGSQLNQSVRSAYQILVDAAVILYFIPFLYMFAAIIRL
                     AARPDRASNPHAVLVPGGRAGVWICGGLGFIVVLIGIVVSAIPPGEATSKLGFEMQLL
                     IGTVISILIGLALYWRGARAK"
     gene            1528..3546
                     /locus_tag="VHE33_08400"
     CDS             1528..3546
                     /locus_tag="VHE33_08400"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVT97514.1"
                     /translation="MIRIQWVFLSATLIAASDVRAQLPTAPQTLRYTFISNGTKSGSE
                     VDTYGAGGRVDSAYEYNDRGRGPKVEAHWILAADGTPQRLDVTGVDYLKAPVDEHFSV
                     AQGQAEWKSASEHGHAAAGGFYAGINSPPLEPALLVRRLVRAGTEGVPLYPSGVAHLE
                     KVQETTVHNASGASMHVTEYAITGLTFDPVGLWLDDQMQFFGTPGSWSALLREGWEQT
                     NDALYALQVKAEQERYHRLAVELTRRPQHAVAIEHVRVFDAPHAAMLDDQTVVVEGDR
                     IRAVGPAAKVQAPADAERIDGRGKTLLPGLFDMHNHVQGGLDGILNIASGVTSIRDMG
                     NGIDVLQRQDERWSKGLEIGPRLWKAGLIDGPGPYQAPTGLFAGTQAEANAAVNRYAD
                     LGYIQTKLYSSFDPKLVPEVIWLSHSRGMRVSGHVPSGMTARQFVEEGADEIQHINFI
                     MLNFLADKVPDTRSTARFTGVGEYAAGVDLDSQPVKDFIALLVQHHTTVDVTLATFEG
                     MFTARLGQVSPQFGPILDRLPPQVQRGAYTGGLPVTAANDETYQRSWQKMLAMTKRMY
                     DAGVPILVGTDDVAGLTLHRELELEVQAGIPALKALQNGTWLAATVLRQQQRLGSVEA
                     GKLADLVLVEGDPSTNISDIRRCRLVLKDGGIFDSGKLYEAAAIRAAR"
     gene            complement(3658..>4012)
                     /locus_tag="VHE33_08405"
     CDS             complement(3658..>4012)
                     /locus_tag="VHE33_08405"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HVT97515.1"
                     /translation="KLAAAMNEDAATKIAARLTELWRTSRPTIMERMAILHSACDSLT
                     RNPLHTEARAQGLEAAHKLAGVLGVFGLPQGSEIASAIEFKLKSEEPLTPGDLADFRD
                     QIADLEAVIASRSEE"
BASE COUNT          797 a         1032 c         1515 g          668 t
ORIGIN      
        1 cttcgcgcgt gcgccgcgcc agtagagcgc caacccgatg aggattgaga tcacggtgcc
       61 gatcagcaac tgcatctcga agccgagttt gctggttgct tctccggggg gaattgcgga
      121 gacgacgatg ccgatgagga cgacgatgaa gccgaggccg ccgcagatcc agacgcccgc
      181 tcgaccgccg gggacgagaa cggcatgcgg atttgaggca cggtcgggac gcgcggcgag
      241 cctgataatc gccgcgaaca tatagaggaa gggaatgaag tagaggatga ccgccgcgtc
      301 cacaaggatc tggtacgcac tgcgtacgct ctggttcaac tggctgccga gcaggatgac
      361 ggcggagatg ccgccctgga cgaggatcga aacccagggg gtcttccagc ggggatggat
      421 ccagccgaac gcgcggggca gatagcggtc gatgccgacg acgaagggga cgcgggcgat
      481 gccggcgacg gtggagccga cgccgccggc gttgcccacg gtaacgagaa tcgccgcgag
      541 gatgccgagg aatccgattt tgagagcgac ggagccgacg gtgatggcgt gaaagacgcc
      601 gctctgggga tcgatcgatg gcgcgggcac gagcgcgagg acggcgaagg ttccggcgat
      661 gtaaacgaag gcgatcatgg cgccggatcc gaggaccgcg cggggcaggg tgcggcgggg
      721 atcgcgaatc tcttcactca tggccgagac gagttcgagt ccggtaaagg cgaaggcgat
      781 ctgggaccag aagttgacgg tcgaccagtt ccaggtgggc agcatgttgc ggagggtgaa
      841 gtgggtcgcg gaaccgaatc cagggccgtg gcgcagggca acggtggcgc cgacggcgag
      901 caggatcagg agagggacat aggtgccgac gccgcccgca ttctgtagcc acttgccgat
      961 gttgaggccg atgatgttca tgcccacagc aacgagcagc aggcccatcg aaacccatat
     1021 ctggaaggcg cggtcaccgg caagacccgc gccgccgggg ccgaggacgt aagcggccat
     1081 cgaagcgctg gcgaggagca ggccggggaa atagaagaag gtgtagatcc agtaggtcca
     1141 gccggcgatg aagccgtgaa agtcgccgaa ggcttctttg gcccagacat agaggccgcc
     1201 ttcttcggga aagcgcgagg agagctcatt gatgacgagc gcgccgggga cgaagaagaa
     1261 gagggcggcg atgacccaga ggctgatgga ggaggtgccg ttatggccgg cggcggcgat
     1321 ccagcggggg ccgaggacag cggcaatgtt gaagagcagc acgtcccaaa agcccatggt
     1381 cttgcgaagc tgggatttgg gttcgggaaa catgtggaag aaggatgata ccgccggaag
     1441 ggagaagccg gtaggaagaa gggagaagcg gcaagctgga agtcagacgc cggaagccag
     1501 acgcgaggtt tgtggtagcg tggcgggatg attcgaatcc agtgggtttt tctgagcgct
     1561 acgctgattg cggcctcgga tgttcgggcc cagctaccga ccgcgcctca gacgcttcgc
     1621 tatacgttta tctcgaacgg aacgaagagc gggtcggagg tggataccta cggcgcgggt
     1681 ggccgtgtgg acagcgccta tgaatacaac gatcgcggac gcgggccgaa agtggaggcg
     1741 cactggatcc tcgctgccga tggcacgcct cagcgcctgg atgtgacggg cgtggattat
     1801 ctgaaggctc ctgtggatga gcacttttcg gtcgcgcagg ggcaggcgga gtggaagagc
     1861 gcctcagagc atgggcatgc ggccgcggga ggtttctacg ccgggatcaa ctcgcctccg
     1921 ctggagccgg cgctgctggt ccggcgactg gtgcgcgcgg ggactgaggg cgtgccgttg
     1981 tacccgtccg gcgtggcaca tctggagaaa gtgcaggaga ccacggttca taacgccagc
     2041 ggcgcatcga tgcacgtgac cgagtacgcg atcacggggc tcactttcga tccggttgga
     2101 ctttggcttg acgatcagat gcagttcttc ggcacgccgg gatcatggag tgcgttgctg
     2161 cgggaagggt gggagcagac gaacgatgct ctctacgcgt tgcaggtgaa agccgagcag
     2221 gagcgttatc accgcctggc ggtggaactg acacggcgtc cgcagcatgc ggtggcgatc
     2281 gagcatgtga gggttttcga tgcgccgcac gcggcgatgc tcgacgatca gacggtggtg
     2341 gtcgaggggg atcggatccg ggccgtggga ccggcagcga aggtgcaggc gccggcggat
     2401 gcagagcgga ttgacggacg cgggaagacg ctgctgccgg gcttgttcga catgcacaac
     2461 catgtgcagg gagggctgga cggaatcctg aacatcgcca gcggcgtgac ctcaatccgc
     2521 gacatgggca acgggatcga cgtgctgcag cgccaggacg agcgctggag caagggtctg
     2581 gagatcggac cgcgtttgtg gaaggcgggg ctgatcgatg ggcctgggcc gtatcaggcg
     2641 ccgacggggc ttttcgcggg aacgcaggca gaggcgaacg cggccgtgaa tcgctatgcg
     2701 gatctggggt acatccagac aaagctttac agttcgttcg atccgaagct ggtgccggag
     2761 gtgatctggc tgtcgcactc acgaggcatg cgcgtgagcg ggcatgtgcc gagcggaatg
     2821 acggcgcggc agtttgttga agagggtgcg gatgagattc agcacatcaa cttcatcatg
     2881 ctgaattttc tcgcggacaa ggtgccggac acgcgcagca cggcgcggtt cacgggcgtg
     2941 ggggaatatg cggcgggcgt cgatctggat tcgcagccag tgaaggattt catcgcgctg
     3001 ctggtacagc accacaccac ggtggatgtc acgctggcga cgttcgaggg catgttcacg
     3061 gcgcggctgg ggcaggtatc tccgcagttc gggccgattc tggaccggct tccgccgcag
     3121 gtgcaacgag gcgcttacac gggcgggctt ccggtgacgg cggccaacga cgagacgtac
     3181 cagcgatcgt ggcagaagat gctggcgatg acgaagcgca tgtacgatgc gggcgttccg
     3241 atcctggtgg gaacggatga tgtcgcgggc ctgactctgc accgtgagct ggagctggaa
     3301 gtgcaggccg ggattcctgc gctgaaggcg ctgcagaatg ggacatggct tgcggcgaca
     3361 gtgttgcgcc agcagcagcg gctcggctcg gtggaggcgg gaaagctggc ggacctggtt
     3421 ctggtggagg gcgatccgtc gacgaacatc agcgatattc gtcgatgccg tctggtattg
     3481 aaggacggag ggatctttga cagcgggaag ctgtatgagg cggcggcgat tcgagcggcg
     3541 cgctgaggcg ggtgcttttg gcgaaaagct gttggcgaaa ggctgctggc taaaaagctg
     3601 ttggcgaaag gctgttagct acaagctgtt ggctaagagc tgttcgctaa aagctgttca
     3661 ctcttccgag cgggaggcga tgactgcctc gaggtcggcg atctgatcgc ggaagtccgc
     3721 tagatcgccg ggggtgagag gctcttcgga cttgagcttg aattcgatgg cggaggcgat
     3781 ttcgctgccc tggggcaatc caaagacgcc aaggacgccg gcgagcttgt gcgcggcctc
     3841 cagcccctga gcgcgggctt cggtatggag aggattgcgg gtgaggctgt cgcaggcaga
     3901 gtggagaatc gccatgcgtt ccatgatcgt cggacggctg gtgcgccaca gctcggtcag
     3961 gcgtgcggcg attttggtgg cagcatcctc gttcatcgct gctgccagtt ta
//
LOCUS       DASZYN010000328         4847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_15106, whole genome shotgun sequence.
ACCESSION   DASZYN010000328 DASZYN010000000
VERSION     DASZYN010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15106"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VHE33_08410"
     CDS             complement(<1..533)
                     /locus_tag="VHE33_08410"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT97516.1"
                     /translation="MTLSSTQAVAAPAWRRVATAGVLGWVLDAFDFFVIVFLFDTLAQ
                     HFHVSKASIVFTLTATLAMRPVGAIVFGALADTMGRKRPLILCVLFFSACTVLSGLAP
                     TYNVFLIFRALYGIGMGGYWGVGASYAIEGSPPGFRGMLSGIIQAGYPVGYLLAAVMM
                     QTLARSLGWRSAFFVGAP"
     gene            761..1096
                     /locus_tag="VHE33_08415"
     CDS             761..1096
                     /locus_tag="VHE33_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97517.1"
                     /translation="MTERTLYRYPFDEEGHEVLTLGESIAAMQELEHQINRLCSLDWR
                     WCDLRLEVIERHPTYVIYEIHHRQEYSGRAVVMPCASPSAMNELRLAISDQSLLTKIQ
                     ARIQAKITQ"
     gene            complement(1141..1428)
                     /locus_tag="VHE33_08420"
     CDS             complement(1141..1428)
                     /locus_tag="VHE33_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97518.1"
                     /translation="MKGKRIARSKPAHRNFRTADRADVPTGRNGKHKSIVTQILSDLE
                     QLESGLCLKIPLSELPDTKANIRSALNRATRKMERPVATATDEEYLYVWNS"
     gene            1831..2244
                     /locus_tag="VHE33_08425"
     CDS             1831..2244
                     /locus_tag="VHE33_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT97519.1"
                     /translation="MRPTECRRILIVDDEKCIADTLALIFRGSGYDAVAYYDGASALA
                     SCATITPDLLITDVTMPGITGIELAILVKQHCPGCKILLFSGLAGSFDLVERAKQQGH
                     DFQLLEKPINPAELLQKVAGTFGIASPLQFPRNVA"
     gene            2546..3529
                     /locus_tag="VHE33_08430"
     CDS             2546..3529
                     /locus_tag="VHE33_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HVT97520.1"
                     /translation="MRRVMMFLNPLLIHNEKRRALVEQIAELLSGHGSDVHLQETLSA
                     HSAGQQARDAVAAGFDTFLVCGGDGTIFQVIQGIAGSDATLGVLPFGTGNVIAQNLHM
                     PRNPLGAARLLLNAQPRDVSLGEVLLSPPGHKRMRTWYFFIAAGMGVHAALMNLSPTG
                     QGKRRGGRLAYFLGGFRLLVDHPIQAFQLEMTLSDGTTQIVPACEAIATHVSEINRWR
                     PGGDPLAPWLRVAWVPPTTRFGLGHASFHALATGKTSTSAGWASLPYPQYATVKRMIC
                     RPLTGTDYRQPLLVEADGEVLGASYTAIGISDKKLRLLWPSEKEPSLNAPA"
     gene            complement(4293..>4847)
                     /locus_tag="VHE33_08435"
     CDS             complement(4293..>4847)
                     /locus_tag="VHE33_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97521.1"
                     /translation="LRGELTSRFLLKVLVVLALSGGVFFYYFGGLRKTGDADSRRRRD
                     RWMTLVSSLAVVLLAMAGFTNLGAPRRQREMRADGQRIMRLYQMSGMVEGYWKTHDST
                     LPADVGQLTGSYLDPVTRRPLTYRPEQGSRYELCANFARSSDGSVDTDAATGADVWVH
                     PAGQHCFELDASVPAGYPTGYVNY"
BASE COUNT         1073 a         1783 c         1161 g          830 t
ORIGIN      
        1 ggcgcgccaa cgaaaaatgc cgacctccag ccgagagagc gagccagcgt ctgcatcatg
       61 accgcggcca gcaaatagcc cacaggataa ccagcctgga tgatgcccga gagcatccct
      121 cgaaaccccg gcggagaacc ctcgatcgcg tacgaagcgc caacgcccca atacccgccc
      181 atgccaatgc cgtacagcgc gcgaaagatc aaaaacacgt tgtacgtggg tgccagcccg
      241 ctcagcaccg tgcaggccga aaagaagagc acgcacagaa tcagtgggcg cttgcggccc
      301 atcgtgtccg ccagcgcacc gaaaacaatg gcccccaccg gccgcatcgc aagggttgcg
      361 gtcagagtga aaacgatgga agccttcgaa acatggaagt gctgcgcaag cgtgtcaaaa
      421 agaaaaacaa tgacgaagaa atcgaatgca tccagcaccc atcccagaac gcccgcggtc
      481 gccacacggc gccaggcagg cgctgcaact gcttgagtgc tgctcagcgt catgcaccgt
      541 tccctcaaat ccgactctca ctgtatagga actcactcaa cgcgcgcctt caggatgcct
      601 cacccccgta aacaatgcag acgcgttact acccaatccg aaagttcctc tttccggtaa
      661 cacatgtcag agagagatac cccattacta caaatcaccc gccggtgact tcgcatccgg
      721 caactttgcg gcgatgatgg tcattcggat tccctgcgcg atgaccgaga gaactctata
      781 tcgctatcca ttcgacgaag aaggtcacga agtcctcacc ctgggtgaat cgatcgccgc
      841 gatgcaggaa ctcgaacacc agatcaatcg cctctgctcc ctcgactggc gctggtgcga
      901 cctgcggctc gaggtcatcg agcgtcaccc cacctacgtg atctacgaga ttcatcaccg
      961 ccaggaatac tccggccgcg ccgtcgtgat gccctgcgcc tctccctcag ccatgaacga
     1021 actcaggctc gccatcagcg atcagtcgct gcttaccaaa attcaggcgc gcatccaggc
     1081 aaaaatcacc cagtgacgaa accatgaact gcaggcagca gactcttctc aggctgccct
     1141 tcaggagttc cacacgtaca ggtactcttc gtcggtcgcc gtggccaccg gcctctccat
     1201 cttgcgtgta gcccggttca gcgccgaacg gatattcgcc ttggtgtcgg gtagctccga
     1261 aagcgggatc ttcaggcaaa gaccgctctc cagttgctcc agatcgctca gaatctgcgt
     1321 cacgatgctc ttgtgcttgc cgttccgccc cgtgggcacg tcggcgcgat ccgccgtcct
     1381 gaaattacgg tgggccggct tgctcctcgc gatgcgtttg cctttcatta tcgaaacgta
     1441 aacataacgt accactaaag aacaaagcgc agaacagaac ggaccgctcc gcccagctat
     1501 cgccgctcaa acctcttctc ccacaaagac ttagcagttt cccctcttaa gaaaaattcc
     1561 ccgtatcgat ccatggcgcg taccgtcagg agtagatcgc ggacggattc ttgaacaaga
     1621 cacctcgaca attcgctacc gtcccactga cagaggtgat ccggaagtta ggcgccacga
     1681 ccaacggaag caagtcgcac agcctgccgg ctggaagctc tccccccaga gtgcctccca
     1741 ccactgcttc cagaaacgca accacaccaa agcgcgtccg atcgtgaccc acagcgcgcg
     1801 ccgagccccc gggtcaggaa agataccgca atgcgcccca ccgaatgcag acgaatcctt
     1861 atcgtcgacg acgagaagtg catcgccgac accctcgccc tcatctttcg cggttccggc
     1921 tacgacgcgg tagcttacta cgatggagcc agcgcactcg cgtcgtgcgc gacaatcacc
     1981 cctgatcttc tgatcaccga tgtcaccatg ccgggcatca ccggaatcga gcttgcgatc
     2041 ctcgtcaagc aacactgtcc gggatgcaaa attctcctct tctccggttt ggccggtagc
     2101 tttgacctcg tcgaacgggc aaaacagcag gggcacgatt ttcaacttct cgagaagccc
     2161 atcaacccag cagaattgct gcagaaggtc gcaggcacct tcgggattgc ttctcctctg
     2221 cagtttcccc ggaacgtcgc ctgaaccatc aagccatccg cgcgcaaaac ccaggcttgc
     2281 gctctgttct aaccggatcg acccgccgcc tcgctcctcg cgaaagttca cccgcgcgcc
     2341 cgcggccttc ctctcctgac cccgttcatc ccggacggct gcccttgatc acccggatac
     2401 gttccgccgc atctcatcat ctgtttacaa tgccgtcagc cattccccgc tagaacgatt
     2461 ctcatatgga gttttccggc ctcgccccca gcgcccttct ctcctccggc cgacccaaac
     2521 cccagccccc cgcgagcttc gattgatgcg ccgcgtcatg atgttcctca acccgctcct
     2581 tatccataat gagaagcgcc gcgccctggt cgaacagatc gccgaactcc tcagcggcca
     2641 tggatccgac gtccaccttc aggaaaccct ctccgcccac tccgccggac aacaggcccg
     2701 cgatgccgtc gccgctggct tcgatacctt tctcgtctgc ggcggagacg gcaccatctt
     2761 ccaggtcatt cagggcatcg ccggctccga tgccaccctc ggcgtcctcc ccttcggcac
     2821 cggcaacgtc atcgcgcaga acctccacat gcctcgcaat cccctcggcg ctgcgcgcct
     2881 cctcctcaac gcgcagccgc gcgacgtctc cctcggcgaa gtcctcctca gccctccggg
     2941 acacaagcgc atgcgcacct ggtatttctt catcgccgcc ggcatgggcg tccacgccgc
     3001 ccttatgaac ctctcgccca ccggccaggg caagcggcgc ggcggccgcc ttgcctattt
     3061 cctgggaggc tttcgcctcc tcgtcgatca ccccatccag gcctttcagc tcgagatgac
     3121 cctcagcgac ggcaccaccc aaatcgtccc ggcctgtgaa gccatcgcca cccatgtctc
     3181 ggaaatcaac cgctggcgcc ccggcggcga tcctctcgct ccgtggctcc gcgtcgcctg
     3241 ggtccctccc acgacacgct tcggactcgg ccacgccagc ttccacgccc ttgccaccgg
     3301 caaaaccagc accagtgccg gctgggccag ccttccctac ccgcaatacg caaccgtaaa
     3361 gcgcatgatc tgccgtccgc tcaccggcac cgactatcgc cagccgctcc tcgtcgaagc
     3421 cgacggagaa gtcctcggcg ccagctacac cgccatcggc atcagcgaca aaaagctccg
     3481 cctcctctgg cccagcgaaa aagaaccctc cctgaacgcc ccggcatagc aagtagcaaa
     3541 gagaacccga agagccgcac cccaaaagcc gcaccccaca gccctcagct caaaaaccgc
     3601 aaccgaaaaa ccgtcacccc aaaaacctgc ggaacgccag gttcacccca cagcaagcag
     3661 ccgactcctt cgccgtccaa atttcaacga gaatgtcctc cgcgggaata cgaaggtacc
     3721 gtcctcgatc ctcccggtgc caattgggga acggatccca ctccgcggac cccaaccctg
     3781 gaaccagctc cttcccatcc ccaaaaaact ccggatcccc gaaaacctgc ggagcgccag
     3841 gttcacccca cagcaagcag cagacccctc atttcgcacg agtcaatcaa tgggatctcc
     3901 aaacgccccc gaaagtacct gttcagtccc gtcccgggaa agaagaacac cggacccgcc
     3961 caggttcaca tccccgcagt tccagttcgg gaaaagaaaa acctgcgcag cgccaggttc
     4021 accccatagc aagcagaaga ctctctcgct ccgcatgagt caatcgattg gatctcatca
     4081 gcgccccgaa agtacccccg cagttccagt tcgggaaaag aaacaacagc ggaacgccag
     4141 gttcacccca cagcaagcag cggactcctt cgcagtcgaa cattcaacaa aaattccccc
     4201 gcgttcaaca cgaaagtacc ccctcgctcc tgtccgtttc attttgggag ggctttccga
     4261 tccgcgccct gtcccaggcc cgcccccacc cctcagtaat tcacataacc cgtgggatat
     4321 cctgccggaa cagaagcatc cagctcgaag caatgctgcc ccgccggatg gacccataca
     4381 tccgctcccg tcgcggcatc cgtgtccacg ctcccatcgc tgctgcgcgc aaagttcgca
     4441 cacagctcat accggctgcc ctgctccggg cgataggtca acggccgccg cgtcacggga
     4501 tccagatagg accccgtcag ctgtcccaca tcggccggca aagtggagtc gtgggtcttc
     4561 cagtaccctt cgaccatgcc gctcatctgg tacaagcgca tgattctctg accatcggcg
     4621 cgcatctccc gctgccgcct cggcgcgccg agattggtaa agccggccat cgccagcaga
     4681 accactgcga gcgatgacac cagcgtcatc caccgatcgc gccgtcgccg cgagtcggca
     4741 tcgcccgtct tcctgaggcc gccgaagtag tagaagaaaa ctccgcctga aagcgcaagg
     4801 accaccagca ccttcagcaa aaacctcgac gtcagctcac cgcgcag
//
LOCUS       DASZYN010000329         3110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1234738, whole genome shotgun sequence.
ACCESSION   DASZYN010000329 DASZYN010000000
VERSION     DASZYN010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234738"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VHE33_08440"
     CDS             complement(<1..273)
                     /locus_tag="VHE33_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97522.1"
                     /translation="MTLGIAVPFPGGSILCTDSPIEANDGSATESEAALMSVTSGKRI
                     FAITYAAKDPRAGRMLASDIATVACSAPTRQDIVPGIKRVMGDWFRS"
     gene            626..1207
                     /locus_tag="VHE33_08445"
     CDS             626..1207
                     /locus_tag="VHE33_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT97523.1"
                     /translation="MVKASTSERITAAAGKLLDRSGAEGVTMRRVANAVGITPMALYR
                     HFANREGLLNQLADAGFKQLASRLEGAAQTGNPEQRLSANLDVFLDFALEKPRLFELM
                     FLQRREGARRFPEDFRAGRSPTARFSAEALEAGMKDGTFRKDDVWEIAFETGAMLQGL
                     VMLYVGGRVSVPEDEFRALVHRAFRRYFHGIRR"
     gene            1191..2639
                     /locus_tag="VHE33_08450"
     CDS             1191..2639
                     /locus_tag="VHE33_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HVT97524.1"
                     /translation="MGFGGKMRDLVMALLAIAATALLMFFGNGLEPHWPLAWFVPLPV
                     LLYALRSSVWRGGAVAFLGMLLGCLNFWNYFRVLSAPAIAWFANFTLMALFFAVGVLL
                     TRALARRGALWSAWIALPAWWVSFEFVRNLLWPHGSGGSVAYTQLNFLPFLQFASLAG
                     PWGMGFVLMLFPTGLALAIHMWCLERRQAGWMLGLTLGVVAAVLTFGAVRLARSQPGP
                     EVKVGIVASDVSQGVADPGASAQRLFEQYAEQAQGLIAHGARVVVMPEDLAVIVDPNT
                     AGADAIFQRVADSTGAVLVVGMNHVSGPIRHNEARIYAPSAPVRSYDKEHLLPPFENI
                     FTPGTSRTTLVRSGQTWGVAICKDMDFTDPARGYGRAGVGLLLAPAWDFQLDAFWHGH
                     IAVMRAVEDGFSLVRSARGGFLTVADDRGRILAEIRSNSAPFATLWATVPAGHDATLF
                     QSWGDWFGWYAVALLALVVLRLVFGPRRSAIS"
     gene            2710..>3110
                     /locus_tag="VHE33_08455"
     CDS             2710..>3110
                     /locus_tag="VHE33_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97525.1"
                     /translation="MKTGIIGLPQVGKTSLFQILTRVKVEGGYSNPREAHLGVAKVPD
                     DRLDRLAALYNPKKLVHATVEYADVAAIGQEALKETAFLTSLRNVDALIHVLRGFEDD
                     SIAHVGAIDPLRDAKSVEFDLMLSDLTQIEKR"
BASE COUNT          522 a          850 c         1046 g          692 t
ORIGIN      
        1 ggaacggaac cagtcaccca tgacccgctt gatgccgggc acgatgtcct ggcgggtggg
       61 agcgctgcag gcaaccgtcg cgatatcaga agcgagcatg cgtcctgcac ggggatcttt
      121 tgcggcgtag gtgatggcaa agatgcgttt gccggaggtg acggacatga gggcggcttc
      181 actttcggtc gccgagccgt cgtttgcctc aatcggagaa tcggtacaga ggatcgatcc
      241 gcctggaaac gggaccgcta tccctaaggt catgcctgcg ggcctttcgc attctttaat
      301 tcgctggacg aagcaggccg cttaagcgcg gctcttgtcc tggtccggtt tccgttcggc
      361 tctccgcgaa gcgctgccga tgaggtcggg tcgtgcgccg tctccgagtg acggcagtaa
      421 gccctgcctt ttattcgata cgcgatgtcg ggaagggtca atgaaatggc gcgcaatatt
      481 tcgaattaag aaccaacgcg ttcaacatcg cagggtgacg ggcagagtgg agcgcacgga
      541 attgcggcgc gagtgctgcg gttcagccgg atgaaacttt gtccaggtat acagcgtata
      601 catctgtgtg gtatacagcg tagacatggt gaaggcatcg acgtccgaaa gaattacggc
      661 agctgcgggc aagcttctgg accggtcagg agccgaaggt gtgacgatgc ggcgcgtggc
      721 aaatgctgta gggatcacgc cgatggcact ctaccggcac tttgccaacc gcgagggact
      781 tctcaatcaa ttggcagacg ccggcttcaa gcagctggca tcacgcctgg agggcgccgc
      841 gcagaccggg aatcccgagc agaggttatc ggccaatctg gatgtcttcc tcgacttcgc
      901 cctcgagaag ccgaggcttt tcgagctgat gtttcttcag cggcgcgagg gcgcacggcg
      961 gttcccggaa gattttcgcg cagggcgatc gccgaccgcc cggttttctg cagaggcgct
     1021 ggaagcgggc atgaaggacg ggaccttccg caaggacgat gtttgggaga tcgcgtttga
     1081 aacgggagcc atgctgcaag gactggtgat gttgtatgtg ggcggccgcg tcagcgtgcc
     1141 tgaagacgaa tttcgcgctc tggttcatcg ggcgtttcgg aggtatttcc atgggattcg
     1201 gcggtaagat gcgggatttg gtgatggctc tgctggcgat cgctgccacg gcattgctga
     1261 tgtttttcgg caacggtctt gagccgcact ggccgctcgc ctggtttgtg cccttacctg
     1321 tgttgctcta tgcgctgcgg agctccgtgt ggcgtggggg agccgtcgcc ttcctgggca
     1381 tgttgctcgg ctgcctcaat ttctggaact acttccgggt cctgagcgcg ccggcaattg
     1441 cgtggtttgc caacttcacc ttgatggcgc tgttctttgc tgtcggcgtg ctgctgacgc
     1501 gggcgctggc acggcgcggg gcgctgtgga gcgcctggat cgcgctgccg gcgtggtggg
     1561 tgagcttcga gtttgttcgc aacttgctat ggccgcatgg cagcggcggc agcgtcgcct
     1621 acacgcagct gaatttcctg ccatttctgc aatttgcttc tttggccggt ccgtggggca
     1681 tgggatttgt gctgatgctg tttcccaccg ggcttgccct ggcgatccac atgtggtgtt
     1741 tggaacggcg gcaggccggt tggatgctgg gattgacgct cggcgtcgtc gctgcggtgc
     1801 tgaccttcgg cgcagtgcgt ctggcgcgct cgcagccggg tcccgaggtg aaggttggga
     1861 tcgtcgcttc agacgtgagt cagggtgtcg ccgatcccgg tgcctccgca caacgactct
     1921 tcgaacaata cgccgagcag gcgcaggggt tgattgcgca tggagcgcgg gtggttgtga
     1981 tgccggagga tctcgccgtt atcgtcgatc cgaacaccgc cggcgctgat gcaattttcc
     2041 agcgagtcgc cgacagcacg ggtgccgtgc tggtggtcgg tatgaatcat gtctcgggac
     2101 cgatccgtca taacgaagca cgcatctacg cgccctctgc gcccgtccgg agctatgaca
     2161 aggagcatct gctgccgcca ttcgagaaca tattcacgcc cgggacttcg cggacgacgc
     2221 tggttcgcag cggccagacc tggggcgtcg caatttgcaa ggatatggac ttcaccgacc
     2281 cggcacgcgg ctacggacgg gcgggcgttg gtttgcttct tgctccggca tgggattttc
     2341 agctggatgc cttctggcac gggcacatcg ccgtgatgcg cgcagtggaa gacggcttca
     2401 gcctcgtgcg ttcggcgcgt ggcggtttcc tgaccgtcgc cgatgaccgg ggacggattc
     2461 tcgccgagat tcggagcaac tctgcgccgt tcgccacgct ttgggcgacg gttcctgctg
     2521 gtcatgatgc cacgctgttc caaagttggg gcgactggtt cggctggtat gcggtggcgc
     2581 tgctggcttt ggtagtcctt cggttggtgt tcggtccacg gcgttctgct atctcctgac
     2641 cgctgactcg ctgcgccatt tatgatcgag aattggcctg agggccaaag tccttattcg
     2701 gaatcgatca tgaaaaccgg cattatcggc ctgccacagg taggcaagac ttcgttattt
     2761 cagattttga cgcgggtcaa agtcgaaggc ggctattcga atccgcgcga ggcccatctg
     2821 ggcgtggcca aagttcccga cgaccggctg gaccggctgg ccgcgcttta caacccgaag
     2881 aagcttgtcc atgccacggt cgagtacgcg gacgtggctg cgattgggca ggaggcgctg
     2941 aaggaaacgg ctttcctgac cagtctgcgc aatgtcgatg cgctgatcca cgtgctgcgc
     3001 gggtttgagg acgattcgat agcgcatgtc ggggccattg atccgctgcg cgatgcgaag
     3061 agtgtcgagt tcgacctgat gctgagcgat ctgacgcaga tcgaaaagcg
//
LOCUS       DASZYN010000330        25752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1039154, whole genome shotgun sequence.
ACCESSION   DASZYN010000330 DASZYN010000000
VERSION     DASZYN010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 25752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25752
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039154"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VHE33_08460"
     CDS             <1..327
                     /locus_tag="VHE33_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97526.1"
                     /translation="TETLFVCFVSSDRRRQQYASDLLQEISQKGITGRRIVVGPGSAQ
                     HLFTTSCDVYLSVAESIDDGYRPVIDVMFGQLLGLHGAVARNVKPDAPSPEGVISRVV
                     QEFRIY"
     gene            complement(374..1807)
                     /locus_tag="VHE33_08465"
     CDS             complement(374..1807)
                     /locus_tag="VHE33_08465"
                     /inference="COORDINATES: protein motif:HMM:NF027442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HVT97527.1"
                     /translation="MKNRSAAIRTLIALALLSELLRLNAQAQKHPNVVVVRPHEIQDV
                     LTNPGMGITTFQRFNGQTLNPPLTWSERGPETKLPQAATKPDFPDTSIAYCRWYWDTI
                     EPEPGKFRWDIIDLALAEAHAHGQTLAIRLMPYSNQDPLPAWYRKSGARRANKPEDKD
                     GSIWQPDFSDPAFLKYWGELVAEAGKRYDGNPYLDSVDISSIGYWGEGWSSYMPDFKY
                     QAPLIDIWLKAFPRTTLLMNFDEPQALTYGTAHGAGWRLDCLGDMRTSSDDPYFPAEM
                     LEIYPQQVVRSGIQDVWQKRPVSLETCGTPSSWKKDGYDVDYILDQALRWHVSSVNVK
                     SSPIPAEWKPNFERFEKRMGYRFILRRMEYPRAVAPGEMMLVHMWWLNAGVAPIYREY
                     PLAIELRSGRDSAVIRVPADVRTWLPGDAVFDGTLYIPDTLPPATYSFRVAMIDPLTN
                     KPAIRLAIDGRDSDGWYDMGTVSVGPR"
     gene            1958..3154
                     /locus_tag="VHE33_08470"
     CDS             1958..3154
                     /locus_tag="VHE33_08470"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HVT97528.1"
                     /translation="MAVLIVAVVLLQPALRAQEVADHGPDTNRLAQIEKRMQEYVDRG
                     EAAGIVTLVEQNGKVIQSAAVGMQNIEENKRMEKDSIFQIMSMTKPFTAIGIMMLAED
                     GKLSLYDNVEKYLPEFHNQKVNVNGWLKPSSRPIRICDLLSHTSGMPNLPQGSMRNLY
                     QTMDQSLADAVREFGTEPLEFQPGTRWLYSNPGMATLGRIIEVVSGQSYESFIEARLL
                     RPLQMKDTFFFPPPDKLGRIAMVYSPNDGKLQRAGADILGGDPGAYRKGAKYPAPEFG
                     LFSTVEDLSHFCEMILGNGEWHGIRFLSPASLKAMSEVQTAGMEAGWYPGADYGLAWE
                     IISRPLGTLGLLPKGSFGHGGAFGTHEWIIPSRNMFTLYMVACNGTCSDTPERAFQAM
                     VQASLQ"
     gene            3317..3913
                     /locus_tag="VHE33_08475"
     CDS             3317..3913
                     /locus_tag="VHE33_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97529.1"
                     /translation="MLTIAISIAALTATVHAQQSREPIHFRLAEVAATYDIERAKIAD
                     ASCSCFWMQGGSAEAAIEFHSAWRVVGSFGGSHASNIEPGVDLSRLTWLVGPRFTYNS
                     SRVTKEITARNESHLFGQILAGGTHAFDSVFPRSGGATSSAGSYAFQAGGGFEVGPAS
                     GLHVRPLELNYVRTALPNSTGNTQNDLQLSFGMVYGFR"
     gene            complement(4322..6694)
                     /locus_tag="VHE33_08480"
     CDS             complement(4322..6694)
                     /locus_tag="VHE33_08480"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HVT97530.1"
                     /translation="MPPAIIVADSSCTHMENPIDTSITRRRFLQSASAICVSTALPLP
                     AFAVPQGADAADPLGWVDPRIGTGGHGHCFPGASLPFGAVQLSPDTFNDGWDWCSGYH
                     ISDSSIMGFSHTHLSGTGCGDLLDFLVMPGVGPVRLVPGSRSNPGEGYRSRFSHDDEH
                     SEPGYYSVLLSDSKVRVELTATERTGLHKYTFPESEDAWLIVDLAHSYLTNGTSSVYS
                     AEMRQVAPDTIAGGHITRAWGQGRHCYFTLQLSKQPTSVVFYSDDKPVHDTRNLIGDC
                     LKAVLHFKTTTGETIYVRTGISGVSAEGAAKNLRAEQPDWDFEKTRTAAQNAWRHQLS
                     RIRADFATPDQKRIFYTALYHMSLGPQLFDDTDGRYRGMDNQIHQLPPNRRNYTTFSL
                     WDTFRAAHPAYTLIQTDRVPDFANTLIRMAEQSPAGMPVWPLQGTETGTMTGYHSASV
                     ISEAIVKDLPGIDTEAAWRVMMQRAIGEDYEGLGYYRRLGYIPADRVSESVSKAFEYC
                     YNDWAIAHVAERLEHNDDAALLRARSASYRHYFNAASGFMEPKLENGEWALPFDPIEM
                     GHSKEWRDYTESNAWQTTFGTMHDAAGTISLFGGREPFLKKLDALFTVPSTLPPDAPP
                     DIAGMVGQYAHGNEPSHHIAYLYVYAGAPWKTQERVRSLMDTMYRNQPDGMAGNEDVG
                     QMSAWYILSALGFYAVDPVSATYVLGSPLVDRAFIELHSGRRLEIEVKRSDPSHAYVQ
                     SFTLNGQRQQRAWFRHADIAGGGHLLFEMGPAPNTSFGADPQQAPPSLKI"
     gene            6730..8199
                     /locus_tag="VHE33_08485"
     CDS             6730..8199
                     /locus_tag="VHE33_08485"
                     /inference="COORDINATES: protein motif:HMM:NF018528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HVT97531.1"
                     /translation="MSHGNWDGGLSRREVLQRAGVAALATLFPRNRSAAGGKGWDPGQ
                     GRPQPGDRRFRCEVVEQYIVRIRARVGDPELAQMFYNCFPNTLDTTVEPGTFDGKPDT
                     AVITGDIPAMWQRDSSAQVWPYLDLASKDDRLRSLLEGVIRRQTRNILIDPYANAFMA
                     DLNSPPLEWSRQDQTDMKQGVGERKYELDSLCYPIRLAHGYWKKSGGTQPFDAQWHDA
                     MRLLVQTMRVQQRKDGPGPYHFQRSAPNPTDSLDDRGYGNPVNPVGLIASGFRPSDDA
                     CIFSFLVPANLFAVRSLRQLAEMANSILHDSALAQQADSLASEVETALRQHGVANTPQ
                     GTIWAYEVDGFGGRVLMDDANVPSLLALPYLNSSPDAELYTRTRAFVWSPQNPWFFRG
                     AAGAGIGGPHEGADMIWPMSQAIYAITSTSVSEIREAVGMLKAGSAGSGFVHESYFKD
                     DPKRFTRAWFAWANTLFGELIAQLAEAHPELLSDYSRAR"
     gene            8689..9531
                     /locus_tag="VHE33_08490"
     CDS             8689..9531
                     /locus_tag="VHE33_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97532.1"
                     /translation="MCARGDTGGFAAVAATAILSLVYGIHPALTRSGIAVTAAALVVM
                     PLLAWFKRRVAQATSSRALAADAVQSGACAYLAGITLAGLVVNAMFRIGWIDSAAALA
                     ALPILIIEGRKAIRGESCCCWSERMFPTAIMGIRLAFSELPGETSHRLACFRVVSRRS
                     TPVLRPGVFPSIVFRYMGFMTAKRGTRLSWVSLLGILCIALVLTTGILSVTHTHGNGQ
                     INHDCALCFTAHQVVQIAFIVTILLASRPIARFSCERFAPLPRQRFVLKLANRPPPVP
                     AVFA"
     gene            9580..12282
                     /locus_tag="VHE33_08495"
     CDS             9580..12282
                     /locus_tag="VHE33_08495"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT97533.1"
                     /translation="MRTYLRVAAVFIFLGFFAAIFPISSYAQAGATSGTITGTVTDPT
                     GAVVKGATVTIENPVSQFRRSEKTDGVGSFTFVNVPFNPYHLAVSGSGFSSSAQDVDV
                     RSAVPVTVPVKLAIGSAATTIQVTAADLLENDTTMHTDVDRNLFETIPLESQSSSLSS
                     LVTLASPGVAADSNGLFHGLGDHAENSFSVDGQPITDQQSKVFSNQLPSSAVQSMEVI
                     DGAPPAEYGDKTSLVIQVTTRSGQGLTKPTGSIYATYGSFGSTTEGFDLGYGKKNWGN
                     FISVDGLNSSRFLDPPEFAVFHDKGNEENVFDRVDYQPNTANSFHLNLNYSRSWFQTP
                     NAYDNLNPGNVNLAGTPVAGQADQRSKIGTFNVAPGYTRVINQYSVFNFGVYLRKDNY
                     HYYGSKDPFADLGPIQQESIGQDRSLANYGVHSDISWVKGRQNVKLGAMYEQTLLREN
                     DALGIVDPGYLDSLTDADGNPCMSGGTPDVALAAPCTDLYPYDLTRGGSFYNFLGRTD
                     VKELALYIEDQIKAGNWLFNLGMRGDLYNGLAAESQAEPRLGISYSIKPTNTVLRISY
                     ARTLETPFNENLVLASRGCLNAVVNPLLGCQGGDTGALTPGFRNEFHAGLQQAVGKHF
                     VVSGDYIWKYTHNGYDFSVLGNTPITFPVEWHNSKIPGIALRADVTQIHNFSAYVVMS
                     SVAARFFPPQVGGAGATVGESGYPFRIDHDEKFNETTHFQYQLPWKHMPWYSFNWRYD
                     SGLVAGAVPCYNVTDPNSLCNPDNDGTSITLPDGRPGIDLSGLTADQQFEAGLTCDGV
                     AATPGSGFTSCDAAGLTSKLVQIPAPGKENDDHDPPRVQPRSLFDMALGEDNLFHGDK
                     YKWGAQISAINVTNKYALYNFLSTFSGTHYVTPRAITGQITLSF"
     gene            12334..12492
                     /locus_tag="VHE33_08500"
     CDS             12334..12492
                     /locus_tag="VHE33_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97534.1"
                     /translation="MAALDPQQRGSKRRLAQIRWKRWELIHVLILAALMTGFGLWVGI
                     WIATHHFD"
     gene            complement(12560..13114)
                     /locus_tag="VHE33_08505"
     CDS             complement(12560..13114)
                     /locus_tag="VHE33_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97535.1"
                     /translation="MRLFVFRITGACLLLLVCSPVNGQSSSAASSRLPQKTIEDKDPK
                     AIVELGAATSWNFSGGAATFAPNLAAEITPIENWLELEAGVSPFYTRKSAEWDTDLLF
                     KKPWTLSRKAEFMLGVGPVWVHLRQNGNVTNSISGETAGDFMFWPSGKHRFGWFLEPA
                     YDYSFAGGHQQSIGMSGGLLIGIP"
     gene            complement(13713..14612)
                     /locus_tag="VHE33_08510"
     CDS             complement(13713..14612)
                     /locus_tag="VHE33_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HVT97536.1"
                     /translation="MPRYEALDDFGRAYFPQAVYEEARLQKEFNLEEIEYSSDGLSVH
                     GLLISPKIPGAQKWPAIIFNRGGNGDLGRITDSGAPCCSVNTSCLDVADLYLLAKAGF
                     VVIASDYRYRDATAKRDEWGGVEVDDILNLVPTLKSLDYVDSDRVYMLGLSRGGTMTY
                     LAIERGVPVKAAAVIGAVTDVKAWVDARPAMNLVNGNMYIDGFANIWPDYKHNAEAEY
                     RARSAVYWADRVNVPVLILHSRTDPMVPVAQALRMAEALQEAGKVYGLRIYEHDGHPL
                     PRNRDDRNRLIVDWFNHAGQGRP"
     gene            complement(14848..15144)
                     /locus_tag="VHE33_08515"
     CDS             complement(14848..15144)
                     /locus_tag="VHE33_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVT97537.1"
                     /translation="MFWATSCSKPLTETEQAGKSLYEIHCFECHEENDLGLKKVPPKL
                     QGLFSHQNLPDGTMPATDAAVRQVIVYGKRTMPAFNGRLSDIQIAELIAYLHRK"
     gene            complement(15225..16154)
                     /locus_tag="VHE33_08520"
     CDS             complement(15225..16154)
                     /locus_tag="VHE33_08520"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HVT97538.1"
                     /translation="MLLISRFGVAALVVGAAMMLLPSGCRKESNNPAGVELHPAAVTE
                     KQYRLQGMVLATSTATSEITIRHGDIPGFMPAMTMVYKVKNPVDVQKLKPGDEITADV
                     LVPSDSDNYLLDRVVITSEKGRKLPPTMLPPHALMVGETVPDVPLVNQDGKLIHLQDY
                     RGKAVLITFIYTRCPMPTACPLITSHFAKVNDLLAKMPAAYAASHLISVSLDPNYDQP
                     PVLRQYGMAYLDDNASAFAHWEFADTTPGDLKKLAQAFGLQYTEEDNQITHTMQTTLL
                     DKDNKVAQQWGGSGWSPSEVANAVAALALQAGR"
     gene            complement(16202..17110)
                     /locus_tag="VHE33_08525"
     CDS             complement(16202..17110)
                     /locus_tag="VHE33_08525"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HVT97539.1"
                     /translation="MNRRELLQRTTALGLTAVGSLALPGATAETFHAAAGSATLNPLT
                     PPANASIPVAFLISQGAVVIDFCGPWEVFEQAAVPGRKFDAFSLYTVAETATPIVASG
                     GLKITPTYTIADAPPPKVIVIPAQSPASEATKAWIRNATRSTDVTMSVCTGAFVLAST
                     GLLNGKPATTHHGAYVDLALQYPDIHVERGARFVETGNLATAGGLTSGIDLALRVVER
                     YFGREVASRTAYALEYQGRGWLNPSSNAEYAGPIRQSANHPVCPICGMDVDRSLSSTY
                     KGKTYYFCEREHKQVFDASPEKFAAP"
     gene            complement(17161..17895)
                     /locus_tag="VHE33_08530"
     CDS             complement(17161..17895)
                     /locus_tag="VHE33_08530"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HVT97540.1"
                     /translation="MNRRSVLKGLASGAGLAISGPSLNPYALAQASGPAPGGPYILPS
                     LPYTYDALEPYIDAQTMHLHHDKHHASYVEHLNAAVAGHPEVAGKSVEELLTHLDTVP
                     ESIRTAIRNQGGGDANHSFWWPTLSRSGGDRPKAELAKDMDAQFGSFDQFQERFSKAA
                     AGVFGSGWAWLTLGGDGKLQIETTANQDSPLTSGRKPILGLDVWEHAYYLKYQNRRPE
                     YVAAFFHVVNWDFVSGQYAAARARNT"
     gene            complement(18025..20421)
                     /locus_tag="VHE33_08535"
     CDS             complement(18025..20421)
                     /locus_tag="VHE33_08535"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT97541.1"
                     /translation="MQSGIWTAIVATACMIAPAPVVHAQQSAALTTSSSGSIAGVLRD
                     PSGALISGARLELRATATSFRETQISDHLGHFSFVSVPAGNYELTVTASGFAGQTLHS
                     LTVAAGKEISEDISLSIPVVAAEVQVEGQPSSIAASILQVQPSDSSQSHNSADMLADV
                     PGVSLHGNGELATIPFLHGLGDERTKIVVDGMTISSACPNHMNPTLSYVAPPQAAQVT
                     VLAGITPVSLGGDSLGGTIAVETPAPVFAEPGGRVHEEGTFSGFYRSNGDNWGGSLSE
                     AIASEHFSLGYVGAFTTTGDYADGAGRKITSTYAQSTDHAVTLGAHGGHNYFVATGSF
                     HHVPFEGFVNAFMDLTHNNATSLNLRYRRTFATGSLDAHFYWQNTTHEMNFLQDKLAV
                     DGADASMPMNTHGRDLGYLVRYETALSARHTVRAGNELHRLRLDDWWPPVAGMAPMMG
                     PNTFININNGRRTRLGTYAEIFSRWNPHWSTLFGARNDTVWSDAGDVHGYSAMYAADA
                     AAFNAENHSRTDVNFDLTAMGRYDANRHAAFELGYARKNRSPNLYERYTWSTGMMAAS
                     MIGWFGDGNSYYGDISLKPETGNVVSGTMLLHGQTPRPWEVKLTPHLNYIHDYIDVNV
                     ASMPMMGSMMGTPLLRFANHDARIYGGDLSGLVTLWGNDSSGSGTLTASGAWLHGTRT
                     DSGTPLYQMMPLNLRLAFDEHTKSFTAGFGSEMVDRKSHLDPNRMELRTPGFALVDVH
                     AVYKTKYVQGGFRTDNLFNRFYELPLGGNNIDLYGATGSMTPVTGRGRSVSVNLIATF
                     "
     gene            complement(20663..21214)
                     /locus_tag="VHE33_08540"
     CDS             complement(20663..21214)
                     /locus_tag="VHE33_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT97542.1"
                     /translation="MSQPERTRRVLVVDDDEVFRTRLCRALAQRNWEATAAANGSQAL
                     AMARESIPDLVLVDLRMPGMDGLSVVEELRAANTSMRIIVLTGYGSIPTTISAMKRGA
                     DYYLSKPADADQILAAYEKLGAPHEAIPDSPAEAPSLARVEWEHMQRVIADCGGNISQ
                     AARALGIHRRSLQRKLSKFPPNR"
     gene            complement(21201..22511)
                     /locus_tag="VHE33_08545"
     CDS             complement(21201..22511)
                     /locus_tag="VHE33_08545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT97543.1"
                     /translation="MPALQKLENDAAPNLALPWVVRLRYAMVLGGGATLGGVAYFLGF
                     YPALLWTTVPLVSVLATNVWLHRVASRSVRAPQTILGFIFLLDTVCLTAVLGLTGGPM
                     NPFSLLYLVQITLAAVVLKKQWVWGLGVLASVCFGLLFVLHVSLKGSHLHSGEPGLSS
                     HLAGMWIAFVIAAALISFFTSRVSNALRLREQEVLALQEHVARQERLTSLVTLAAGAA
                     HELGTPLGTIAVVARELELYASTLENSDAVCDDAKLIRSEVERCEHILQRMSADGAEP
                     VGESPRAIQIGELLRQTLREIPEPRRSQISIDADGAPTDLFLPVRATVQSLTALLSNA
                     LDAGSEARITLRAQSRGSEIVFSVCDHGSGMSNETLRRIAEPFFTTKEPGKGMGLGTF
                     LVRTFAQRLGGELRYQSALGNGTTATLTLPVHFSKQDQSHRVST"
     gene            22966..23418
                     /gene="nikR"
                     /locus_tag="VHE33_08550"
     CDS             22966..23418
                     /gene="nikR"
                     /locus_tag="VHE33_08550"
                     /inference="COORDINATES: protein motif:HMM:NF003381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HVT97544.1"
                     /translation="MGDLSRIGIALDSDLLRRFDKSIARRGYTNRSEAFRDLIRDRLV
                     GEQTAMPDATVVGTVTLIYDHHAHSVGEKLTEIQHEHHQLIVSTSHAHLDHDSCLEVL
                     IVHGRSSQVERLADRLIGLKGVQHGRLVMTAPQHPLGPPEHKVHKHAK"
     gene            23437..>25752
                     /locus_tag="VHE33_08555"
     CDS             23437..>25752
                     /locus_tag="VHE33_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT97545.1"
                     /translation="MGLCRRTGSTIFRFVLLLFLSSLTGVVPLHAAAQEKTSVTANDG
                     LPAPQLSGNVVDASGAVIAGTTVQVRSANGAVLRTMQTDRDGCFNIYGLPAGHYQIVI
                     SHPDFETREMPVTITTARTAATLGITLTLSAVSTTINVQGRADDLIGIADSATLGTVG
                     AREIEDRPILRSGEILETIPGVIITQHAGGGKANQYFLRGFNLDHGTDFAVFTDGVPL
                     NLPSHAHGEGYADMNTVIPEFVKRVDYEKGPYFADVGNYGSAGSAHLEFFKILPGNFF
                     RLEGGMFGYGRMLFGASQKLGPGNLLYGGEAYHDDGPWTHPDNYYKFNGLLTWSQGGD
                     DHGFSITARGYHGTWHSSDQIPVPAVPLVGLFGSLNPTDGGHSRRYSLQGEWHRLDAH
                     SMTRVVIYGFYYDLDLFSDFTYYLTDPSRGDQFEQQDRRWVAGLDARHTIFSQWLGRR
                     VENTFGVQIRNDWVHNGLYQSEHRVRVDKTDADTGTTLPATTQADRFTDTQPGFYAEN
                     TIHWADKFRSDAALRGDLESFDVTSLVTSANSGAASKWLPSPKLSLVFGPWATTEFYA
                     QGGFSFHSNDGRGTTQTIEPISGENPYPNTPADKIPPLIPTKGGEIGVRTAAVPRLQS
                     TFSLWYLHSASELQQSGDTGGTTASRQPSNRYGIELANYYTPAEHWAVDLDLAHSKAL
                     FTEIDGDDAAPGSPGGKRVPEAAGLVIASGITLHDYRGFSSSLRLRYFGARDLTSDGL
                     FRSKATALVNGQVGYQIGETWRVSAEFLNLLN"
BASE COUNT         5307 a         7313 c         7873 g         5259 t
ORIGIN      
        1 acagagacat tgtttgtgtg cttcgtttcc agcgatcgaa ggaggcaaca gtacgcaagc
       61 gatcttttgc aggagattag tcaaaaggga attacagggc gaagaattgt agtcggcccc
      121 ggttcggcac agcatctgtt caccacatcc tgcgatgtct atctctccgt cgcagaatct
      181 atcgatgacg gataccgtcc agttatcgat gtcatgttcg ggcaactcct tggtctccac
      241 ggtgcagtag ccagaaatgt taagccagac gcgccgagcc ctgagggagt tatcagccgg
      301 gtcgttcagg agttcaggat ttactgaagc cgaactgctt gatcggtgca gatagctctg
      361 gaatcttacg ggactaacga ggtcccacgc ttactgttcc catgtcgtac caaccgtctg
      421 aatcgcggcc gtcaattgcc aggcggatcg ccggcttgtt ggtcagggga tcgatcatgg
      481 cgacacggaa actgtaagtt gccggcggca gcgtgtcggg aatgtagagt gtgccgtcga
      541 acactgcatc gccaggcagc catgtgcgaa cgtcggccgg cacacggatc acggctgaat
      601 cgcggccaga acgcagttcg atggcgagcg gatattccct gtaaatggga gcgacgccgg
      661 cattcagcca ccacatatgc accagcatca tctcaccggg agctacggcg cggggatatt
      721 ccatgcggcg caggatgaag cggtagccca tcctcttttc aaagcgctca aagtttggtt
      781 tccactccgc aggaatcggt gaggatttca cattcaccga actgacatgc caccgcagtg
      841 cctggtccag gatgtaatcc acatcgtatc catccttttt ccaactcgag ggagtcccgc
      901 aggtttcgag tgagacgggc cgcttttgcc acacgtcctg aattcctgag cgcaccacct
      961 gctgagggta gatctcgagc atttcagcgg ggaagtaagg atcatcggaa ctggttcgca
     1021 tgtctccgag gcagtcgagt cgccagcccg caccatgggc ggtgccgtag gtgagcgcct
     1081 gtggttcgtc gaagttcatc agcaaggtag ttctggggaa ggccttcagc cagatatcga
     1141 tgaggggggc ctggtatttg aagtcgggca tgtagctgct ccagccttcg ccccaatatc
     1201 cgattgagga gatatccacg ctgtcgaggt acggatttcc gtcgtagcgt ttcccggctt
     1261 cggcgactaa ctcgccccag tacttgagga aggcaggatc ggagaagtcg ggctgccaga
     1321 tcgaaccgtc cttgtcttcc ggtttatttg cacgccgcgc gcccgatttc cgataccagg
     1381 caggaagtgg atcctgattg gagtaaggca tcagccggat ggccagagtc tggccgtgag
     1441 cgtgagcctc ggcgagcgcc agatcgatga tgtcccaccg gaacttaccg ggttccggtt
     1501 caatcgtgtc ccaataccag cggcaatagg caatcgatgt atcggggaag tcgggcttcg
     1561 tggccgcctg gggcagcttg gtttcgggtc cgcgttccga ccaggtcaac ggtggattga
     1621 gcgtctgccc attgaaccgc tgaaaggtgg tgatacccat ccccggattg gtgaggacat
     1681 cctggatctc gtgcggccga accacgacta cattggggtg cttctgggcc tgcgcattca
     1741 acctcaacaa ctccgaaaga agggcgaggg cgatcaaggt tcgaattgcc gcggaacgat
     1801 ttttcatgag ccagccgtgt gtcgaccatg ataacgtttc atatcgtttt agagcgcaga
     1861 aaaggaaaga aattcataaa cgatggaaaa cgatagcatt ctgtcttgcc aacgaacctt
     1921 cggaacatgt ttctcctgga accggccggc ggtctgtgtg gcagtgctga tcgtcgcggt
     1981 agtcctgctg caacctgcgc ttcgagcgca ggaggtcgcg gaccacggac cggacacgaa
     2041 ccgccttgca caaatcgaga agcggatgca ggagtatgtg gatcggggag aggctgccgg
     2101 gatcgtcacc ctggtggagc agaacggaaa ggtgatccag tcggccgcgg tcgggatgca
     2161 gaacatcgaa gagaacaagc ggatggagaa agattccatc ttccagatca tgtccatgac
     2221 caagcctttt acagcgatcg gaatcatgat gctggcggag gacggaaagc tgagtcttta
     2281 cgacaacgtc gagaagtatc tgccggagtt ccataaccag aaagtgaatg taaatggctg
     2341 gctgaagcct tcgtctcgcc ctattcgaat ctgcgatctg ctctcccaca cctcaggcat
     2401 gccgaacctg ccgcagggct cgatgagaaa tctctaccaa acgatggatc agagcctggc
     2461 tgacgcggtg cgggaattcg gcaccgagcc gctcgagttc cagcccggca cgcgatggct
     2521 gtacagcaat ccggggatgg cgacgctggg gcgcatcatc gaggttgtct cggggcaatc
     2581 ctatgaaagc ttcattgaag cgcgcctgct gcggccgctc cagatgaaag acaccttctt
     2641 ctttccccct cccgataagc tgggccgcat cgccatggtg tactcgccca acgatggcaa
     2701 gttgcagcgc gccggagccg acatcctcgg tggcgatcct ggggcttatc gcaagggagc
     2761 gaagtacccc gcgcccgagt tcgggctgtt ctcgaccgtg gaagacctgt cgcacttttg
     2821 cgagatgatc ctgggcaacg gtgaatggca cggaatccgg tttctctcac cggcaagtct
     2881 aaaagcgatg agcgaggtcc agactgcggg catggaggca ggatggtatc ccggggccga
     2941 ttatgggctt gcatgggaga tcatcagccg gccgctcggg acgctgggat tgttgccgaa
     3001 aggaagcttc ggtcacggtg gcgcattcgg aacccatgag tggatcatcc ccagccgaaa
     3061 tatgttcacg ctctatatgg ttgcgtgcaa tggcacgtgt tccgatacgc cggagcgggc
     3121 atttcaggca atggtgcagg ccagtctgca gtagggcatt ttcagtgccg caagggagaa
     3181 ggtttccaaa tacgctgttt tgatttgcgt cccgacagga tttgaacccg cgtatcgccg
     3241 tgaaagaacg cggggaaatc gtagctctaa cattccagag gcagcgaggt gcatcggaat
     3301 cggcaagccg ccagccgtgc tgacgattgc gatatcgatc gcagctctca ctgcgaccgt
     3361 acatgcgcaa cagagcagag agccaatcca tttccgcctg gcagaagtcg cggcgaccta
     3421 cgatatcgaa cgtgcgaaga tcgcagacgc atcctgcagt tgtttctgga tgcagggagg
     3481 cagtgctgaa gcggcgatcg agttccattc ggcgtggagg gtggtgggca gctttggggg
     3541 ttcacatgcc tcgaacattg agcccggcgt ggacctgagc cgtctgacgt ggctggtcgg
     3601 gccgcgtttc acctacaaca gttcgcgagt cacaaaagag atcaccgcca gaaacgagtc
     3661 acatcttttt ggccagatac tggccggagg cacgcatgcg tttgacagtg tcttcccccg
     3721 aagcggcgga gccacgtctt ctgccggttc gtacgcattc caggcgggag gcgggtttga
     3781 agtggggcca gccagcggcc tccacgtgcg tccccttgag ctcaactacg tgcgcaccgc
     3841 actgcccaac tcgaccggca atacccagaa cgatcttcaa ctgagtttcg ggatggttta
     3901 cggcttcagg tagtccggat acgattccgc gaggggcctg acgaaggtac ttacagttac
     3961 gaccgggccc gtcaaccctt gcccttcgat ccgcctgtgc tcgcgctaca gttccgtggt
     4021 cggcgtctgc accggcgcgc aactcggcgg tcacgccggc gaactgcgca agcggattga
     4081 tgaccgtcgc cgtcagcggt atgactaatt accgatgagt aatccttgcg ccgggcgaat
     4141 gtcgccagcg ctggacaacg atggggcggc tttccaccgt ttgtgactga gggctgaggt
     4201 cgatttgtcc gatgcctggt tggcatcccg ccgcaagtgg acgatgaagg ccagccttga
     4261 tgacaacgct ggagtgaaga ctggccattt gaaaggcaag gacggcagct gctcatgcag
     4321 gctatatctt taaagaaggc ggagcctgtt gcggatccgc accgaaagag gtgttcggcg
     4381 cgggacccat ttcgaacaag aggtggccgc ctccagcgat gtcggcatgg cgaaaccacg
     4441 cgcgctgctg acgctggcca ttgagggtga acgactgtac ataggcgtgg gatggatcgc
     4501 tgcgcttcac ttctatctca aggcgcctcc cgctgtgcag ttcaatgaac gcgcgatcga
     4561 ccagtggtga gccgagcaca taagttgcac tgacgggatc cacagcgtag aagccgagcg
     4621 ccgagaggat ataccacgcg gacatctggc cgacgtcttc gtttccggcc attccgtcgg
     4681 gctggttgcg gtacatcgta tccatcagag agcgaacacg ctcctgggtc ttccatggag
     4741 cgccggcata gacgtacaga taggcaatgt gatgggaagg ctcgttgccg tgcgcatact
     4801 ggcccaccat gcccgcgatg tcgggcgggg catctggcgg cagggtggag ggaaccgtga
     4861 aaagcgcatc gagtttcttc aggaaaggct cgcgtcctcc gaagagggag attgtgccgg
     4921 ctgcgtcatg catggttccg aaggtggtct gccaggcgtt ggattcggta tagtcgcgcc
     4981 actctttgct gtggcccatc tcgatgggat cgaagggcag agcccactcg ccgttctcga
     5041 gtttaggttc catgaaacca ctggccgcgt tgaagtagtg ccggtagctg gcagagcgcg
     5101 cgcggagcag ggcagcatcg tcgttatgct caagcctctc ggcgacgtgt gcgatggccc
     5161 agtcgttgta gcagtactcg aaggcctttg acaccgattc gcttacacgg tctgcgggaa
     5221 tatagccgag gcggcgatag tagccgagtc cctcatagtc ttctccgatg gcacgctgca
     5281 tcatcacccg ccaggccgct tcggtatcaa ttccgggaag atccttcacg atggcttcac
     5341 tgatgacgct ggcggaatga tagccggtca tggtgccggt ctcggttccc tgcagaggcc
     5401 agacgggcat gcccgccgga ctttgttcgg ccatgcgaat cagggtgttg gcaaaatcgg
     5461 gcacgcgatc ggtctggatg agggtatagg ccgggtgggc ggcgcggaag gtatcccata
     5521 acgagaaggt ggtgtagttg cgacgattcg gcggcagctg atgaatctgg ttatccatgc
     5581 cacgatagcg gccgtcggta tcgtcaaaaa gctgcgggcc gagcgacatg tgatacagcg
     5641 cggtgtagaa gatgcgcttc tggtctggag tggcgaagtc tgcgcggatg cgagatagct
     5701 gatggcgcca ggcattctgc gcggcggtgc gggtcttttc gaaatcccag tcaggctgtt
     5761 cggcgcgtag gttcttcgcc gcgccttcgg cgctgactcc ggagatgccg gtgcgaacgt
     5821 agatcgtttc tccggtggtg gttttgaagt ggagtacggc cttgaggcag tcgccgatca
     5881 gattgcgggt gtcgtgcacc ggcttgtcat cggagtagaa gacgacgctg gtgggttgtt
     5941 ttgagagctg cagcgtgaaa tagcagtgcc tgccctgccc ccacgcccgg gtgatgtgtc
     6001 cgccggcgat cgtatcgggc gcaacctggc gcatttccgc tgagtaaacg gaagaagtgc
     6061 cgttggtgag atagctgtga gccagatcga cgatcagcca ggcgtcttca ctttcgggga
     6121 acgtgtattt gtgcaggccg gtgcgctcgg tagccgtcag ttcgacgcgc actttcgagt
     6181 cgctgagcag aacagagtaa tagccgggct cggagtgctc gtcgtcatga gagaagcgcg
     6241 aacggtagcc ttcaccgggg ttggagcgcg agcctggcac gagcctgacc ggacccacgc
     6301 caggcatgac gagaaagtcg agcagatcgc cgcagccggt gccgctcagg tgcgtatggc
     6361 tgaaacccat gatggagcta tcggaaatgt gatagccgga acaccagtcc cagccgtcgt
     6421 tgaaggtgtc ggggctgagc tgcacggcac caaagggaag gctggctcca gggaagcagt
     6481 ggccgtgtcc gccggtaccg atgcgagggt cgacccaacc gagtggatcg gctgcgtcgg
     6541 cgccctgagg aacagcaaag gcaggaagag ggagagcggt ggagacgcag attgcgctcg
     6601 cggattgcag aaagcggcgg cgtgtaattg aggtatcgat gggattctcc atatgagtgc
     6661 agcttgagtc tgcaacaatt attgctggtg gcattctaaa ttcctccgca gcgtaaggag
     6721 acctctgagt tgagtcacgg gaattgggat ggagggctat cgcgccgaga ggtactgcag
     6781 cgcgccggag tggcagcgct ggctacgcta tttcctcgca acagatcggc ggcaggaggc
     6841 aagggatggg atccgggcca ggggaggccg cagccaggag atcgccgatt ccgctgcgag
     6901 gtagtcgagc aatatattgt ccgcatccgt gcgcgcgtcg gcgatccgga actggcgcag
     6961 atgttttaca actgcttccc aaacacgctc gatacgacgg tggagcctgg gacattcgat
     7021 ggcaaaccgg acacggcggt gatcaccggc gatattcccg ccatgtggca gagggactcg
     7081 tcggcgcagg tgtggcctta tctcgacctt gccagcaaag atgaccgtct gcgatcgctg
     7141 ctcgagggtg tcatccggcg gcaaacgcgc aacatcctga ttgatcccta cgccaatgcg
     7201 ttcatggcag acctgaatag tccgccgctg gagtggagcc gccaggatca gactgatatg
     7261 aagcagggcg tgggggagcg caaatacgaa ctggactcgc tgtgctatcc gatccgtctg
     7321 gcccacgggt actggaagaa atccggcgga acgcagccat tcgatgcgca gtggcatgac
     7381 gcgatgcggc tcctggttca aaccatgcgc gttcagcagc gcaaagacgg acccggccca
     7441 tatcattttc agcggtccgc gccgaatccg accgactccc tcgacgatcg gggctatggc
     7501 aatccggtca atccggtggg gttgatcgcg tccgggtttc gcccatcgga tgatgcgtgc
     7561 attttctcgt ttctggtgcc ggcgaacttg ttcgcggtga ggtcgctgcg ccaattggcg
     7621 gaaatggcaa attcgattct tcacgacagt gcgctggcac agcaggcgga ttcgctggcg
     7681 agcgaggtgg agacggccct gcggcaacac ggagtcgcga atacacctca ggggacgatc
     7741 tgggcctatg aagtggacgg atttggcggc agagtgctga tggacgatgc gaatgtgccg
     7801 agcctgctgg cgctgccata tctgaatagt tcgccggatg ccgagctata tacacgtaca
     7861 cgagcctttg tctggagtcc gcagaatccg tggttttttc gcggggcggc gggagcggga
     7921 atcggaggac cccacgaagg ggccgatatg atctggccga tgtctcaggc gatctacgcg
     7981 atcaccagca cttcagtgag cgagattcgc gaagcagttg gcatgttgaa ggccgggtca
     8041 gcggggagcg ggtttgttca cgaaagctat ttcaaggacg atccaaaaag atttacgcgg
     8101 gcctggtttg catgggcgaa cacgctgttt ggcgagttga tcgcgcagct ggccgaggcg
     8161 caccctgaac tgctgtcgga ttattcacgg gccagataag tgctgtctcg tgggggttga
     8221 tttggtcttc gactggattt gaagacgggt tatcggcgtg aaagattact ggaaaatcgt
     8281 aaccctaagg cctcgtatcg tgtcacgcct ttccgcgagc caacggactg ggtcttcgcg
     8341 actgacagcg cggacgggcc agaagcaggg caagcagccg ctgtggctgg cgaagcgggt
     8401 cgccaggcat acgttccggc ggaccttcac cgggcaggat ccatccacac tggccgactt
     8461 ctcggtaccg attcggggag catcgagcag gctgggcgtg tggcttttat ccggcaacac
     8521 cgtacaaaga agaaaaccat ggcagctcgg gcgaatatga cacggcaagc accagtctct
     8581 tgcgcggatg cggaccgcgt ccgcgaggtc gcgagttagg atcagtgcgc gcgtgctgcc
     8641 actggcacgc aaagagaatt cgcccggcgg gcgccggaca atctggacat gtgcgcccgg
     8701 ggcgataccg gaggctttgc agccgttgcc gccactgcga ttctttcact cgtatatggt
     8761 attcaccctg cactgacccg cagcgggatt gccgtcactg ccgctgctct cgtcgtcatg
     8821 ccactgcttg cctggttcaa gcggcgggtg gcgcaggcaa cgagtagccg tgcacttgcc
     8881 gcggatgcag ttcagtcggg agcctgtgcc tatctggctg ggatcaccct tgctggtctg
     8941 gttgtaaacg ccatgttccg aattggctgg atcgattctg cagcggcact ggcggctttg
     9001 ccgatcctca tcattgaggg acgaaaggca atccgcggcg agagctgctg ctgctggtca
     9061 gaacggatgt ttccgacagc cattatgggc atccgactcg cgttttcgga actgcctggt
     9121 gagacgtctc atcgcctcgc gtgtttccgg gtcgtaagcc gccgaagcac gccggttttg
     9181 cggccaggtg tctttccgtc tatagtcttt agatacatgg gcttcatgac ggcaaagcgc
     9241 gggacacgac tttcctgggt atccctcctg ggaattctct gtatcgccct tgtccttacc
     9301 accggcattc tgtcggtcac tcacacccat ggcaacgggc agatcaatca cgattgcgct
     9361 ctgtgcttca cggcccatca ggtagtccag atcgccttta tcgtgacgat cctgctggcc
     9421 tcgcgtccga ttgcgaggtt cagctgtgaa aggtttgccc ccctcccacg tcagcgcttc
     9481 gttctgaagc tcgccaatcg gcctccgcct gtccctgctg ttttcgccta acacagtagt
     9541 tcaccccaga ggcattccat tcaaaatcgg aggtcgttta tgcgcacgta tctgcgtgtc
     9601 gctgctgttt ttatttttct cggtttcttc gccgcaattt tccccatttc ttcctatgct
     9661 caggccggag cgacatccgg cacgattaca ggcacggtga cggatccaac cggggccgtc
     9721 gtgaaaggcg caacggtcac catcgagaac ccggtcagtc agttccggag aagcgagaag
     9781 accgatggtg tgggcagctt tacgtttgtc aacgtgccgt tcaatcccta tcacctggct
     9841 gtatccggat ccggcttcag ttcttccgcc caggacgtgg atgtgcgctc ggcggtaccg
     9901 gtgacggtgc cggtgaagct ggcgattggt tcagcggcaa cgacgattca ggtgacggct
     9961 gcggacctgc ttgagaacga cacgaccatg cacactgacg tggaccgcaa ccttttcgag
    10021 acgattccgc tggagagcca gtcctcctcg ctcagttcgc tggtgacgct ggcgtcgccg
    10081 ggcgtggcgg ccgattcgaa cggacttttt cacgggctgg gagatcatgc ggagaattcc
    10141 ttctccgtgg acggccagcc gattaccgat cagcagagca aggttttctc gaaccagctt
    10201 ccctcatcgg cggtgcaatc gatggaggtg atcgacggag ctccgcctgc ggagtacgga
    10261 gacaagacca gcctggtgat ccaggtcacg acgcgctccg gccagggatt gaccaaaccg
    10321 acgggatcga tctacgcgac gtatggttct tttggttcga cgaccgaggg attcgacctt
    10381 ggctacggca agaagaactg gggcaacttc atatccgtgg acgggctgaa cagcagccgc
    10441 tttctcgacc cgccggagtt cgccgtcttc cacgacaagg ggaacgaaga gaacgtattc
    10501 gaccgggtcg actatcaacc aaacacggcg aattcgttcc atttgaacct gaactactcg
    10561 cgctcgtggt ttcagacacc gaatgcctac gacaatctca accccgggaa cgtcaatctc
    10621 gcgggaacac cggttgcagg gcaagccgat cagcgctcga agattggaac gtttaacgtc
    10681 gcgccgggtt acacacgggt aatcaaccaa tattcagttt tcaacttcgg ggtatacctt
    10741 cgcaaggata actaccacta ttacggcagc aaggatccgt ttgcggatct gggaccgatc
    10801 cagcaggagt ccattgggca ggatcgcagc ctggcgaact acggcgtgca ttccgatatt
    10861 tcctgggtca agggccggca aaacgtgaag ctgggggcga tgtatgaaca aaccctcctc
    10921 cgtgaaaacg atgctctcgg tattgtcgat cccggatatc tggattcgct caccgatgcg
    10981 gacgggaacc cctgtatgtc ggggggcacg ccggacgtgg cgctcgctgc accgtgcacg
    11041 gatttgtatc cgtacgatct gacccgaggc ggctcgttct acaacttcct gggtcgaacg
    11101 gacgtgaagg agctggcgct gtatatcgag gaccagatca aagccggcaa ctggctgttc
    11161 aaccttggca tgcgaggcga tctttacaac gggctggcgg cggaaagcca ggcggaaccg
    11221 cggctgggta tctcgtacag catcaagcca acgaacacgg tgttgcggat ctcgtatgcc
    11281 cggacgctgg aaactccgtt caacgagaac ctggtgctgg cgagccgcgg gtgcctgaac
    11341 gccgtagtca atccgctgct ggggtgccag ggcggcgata cgggggcgct cacgccgggt
    11401 ttccggaacg agttccatgc cgggttgcag caggcggtgg gcaagcattt cgtggtcagc
    11461 ggcgactaca tctggaaata cacgcacaac ggttacgatt tcagcgtttt ggggaacact
    11521 ccgattacct tcccggtgga gtggcacaac tcgaagattc ccgggattgc tctccgcgcc
    11581 gatgtgaccc agattcacaa cttcagcgcg tatgtggtca tgtcgtcggt ggccgcgcgc
    11641 ttcttcccac cgcaggtggg gggcgcgggc gcgacggtgg gggagagcgg gtatcccttc
    11701 cgcatcgacc acgacgagaa attcaacgag acgacccact tccagtacca gctaccgtgg
    11761 aagcacatgc cctggtacag cttcaactgg aggtacgaca gcggcctggt agcgggagcc
    11821 gttccctgtt acaacgtgac ggacccgaac agcctgtgca acccggacaa cgacggtacc
    11881 tccatcaccc tccccgatgg acggcccggg atcgatctga gcgggctgac ggcggaccag
    11941 cagttcgaag cgggtctgac ctgcgacgga gtggcggcga cgccaggctc agggttcacg
    12001 agctgcgatg cggcggggct gacctcgaag ctggtacaga ttccagcgcc cggtaaggaa
    12061 aatgacgacc acgacccgcc gcgggtccag ccgcgtagcc tgttcgacat ggctcttggc
    12121 gaggacaacc tcttccacgg cgacaaatac aagtggggcg cgcagatttc ggccatcaac
    12181 gtcaccaaca agtacgcgct ctataacttc ctctccactt tcagcggcac gcactacgtc
    12241 acaccgcgtg ccatcacggg ccaaatcacc ctctcgttct aaagcccgat aggggcggac
    12301 cgatttccgc ccctatcgat tcgggaggtc tatatggccg cactcgatcc gcagcagcgc
    12361 ggaagcaagc ggcgcctggc ccagattcgg tggaagcgct gggagctcat ccatgtgttg
    12421 atcctcgccg cgctgatgac cgggtttggc ctttgggttg gcatctggat cgcaacccat
    12481 catttcgatt gagccgggca tgtccagcca cgatgctgcg gtgtcaggca gcgcgcgctc
    12541 ggctcattct ccctgacctt cacggtattc cgatgagtag ccccccgctc atgcctatgg
    12601 attgctggtg acctcccgca aagctgtagt catacgccgg ttcgagaaac cagccgaaac
    12661 ggtgtttgcc agagggccag aacatgaagt ctcctgccgt ctctccggag atggagttcg
    12721 tcacatttcc attctgtctg aggtgcaccc atacaggacc gacccccagc atgaattctg
    12781 cctttcgtga caaagtccac ggctttttga aaagcaagtc ggtgtcccat tccgctgact
    12841 tgcgagtgta gaagggcgac acgcctgcct caagctccag ccagttctcg atgggtgtga
    12901 tctctgcggc aagattcggc gcgaacgttg ctgcgcctcc gctgaagttc cagctcgtcg
    12961 cggcgccaag ctcaacaatg gctttggggt ctttgtcctc aatggtcttt tgaggaagac
    13021 gcgaggatgc agctgaggaa ctctgcccgt taacgggcga acacacaagc agaagcaggc
    13081 acgctccagt gatcctgaac acgaacagac gcatagatgt ctccaaagga agtggtgcgt
    13141 tccaatatcc gcgctggaag cacggaaagg ccaggctaag cggaggcatc tgtacgggga
    13201 ggtcgaaaga tggatgatcg ggacgtgaaa acagttgagg gctgctgagc ccagacataa
    13261 gacgtcgcgg tattgacccg gccaaaatca aaacctgcat ttggcagacc gtgaaagccg
    13321 cagcaccagc agatgccgtt acaaggctcc ggagtagtcg gttgctgctg ggtgccaata
    13381 gcctgcgtcg aagaagaggt ccctgtgagg ggtccatcgc acagatcgca gtgcggcgga
    13441 tcgaccgcca ggcacaacaa aaccgcgcac aacagccatg cgatgaggta agccggcgtt
    13501 gcaattgcgc ggttcatgcc tgtaaagata acgcttgatg agacagatca ccagcggctc
    13561 aaccggagga tgccgctgag gaactctgcc cgttagcggg tgagcacaca agcagaagca
    13621 ggcacgatcc actgatcctg aacaaccgcg cataacagcc atgagatgag gtaaccctta
    13681 atgaagccat gcttacatca tcgccatgtg gactaaggtc ggccctgtcc ggcatggttg
    13741 aaccagtcga cgatcaggcg attgcggtcg tctcggtttc gtggcagcgg atggccatcg
    13801 tgctcgtaaa tgcgaagacc gtacactttg cccgcttcct ggagcgcttc cgccattcga
    13861 agagcctgcg caactgggac catggggtcg gttctgctgt gaaggatgag gacgggcaca
    13921 ttgacccggt cagcccaata gacggcagat cgcgctcgat actccgcctc tgcgttgtgc
    13981 ttgtagtcgg gccagatatt tgcgaatccg tcgatataca tattcccgtt gacgaggttc
    14041 atcgcgggcc tcgcgtcaac ccaggctttc acgtcggtga ctgcaccaat gactgccgca
    14101 gctttaactg gaacacctcg ttcgatcgcc aggtaagtca tcgtaccgcc tcgtgaaagg
    14161 ccgagcatgt acacccgatc cgaatcgacg tagtcgagag atttcagcgt cggcacaagg
    14221 ttgagaatgt cgtccacctc cactccgccc cactcgtcgc gtttcgccgt agcgtccctg
    14281 taccggtagt cggaggcgat gacaacaaaa ccagctttgg cgagcaggta gagatcggca
    14341 acgtccagac acgatgtatt cacactgcaa caaggtgcgc cggaatcggt gatacggccc
    14401 aggtcaccat tccctccacg attgaagatg attgcaggcc atttctgagc ccccgggatc
    14461 ttcgggctga tgagtaaacc atgcacagaa agcccgtcgc ttgagtactc gatctcctcg
    14521 aggttgaact ctttttgcaa gcgtgcctct tcgtagactg cctgcgggaa ataggcacgc
    14581 ccgaagtcgt cgagggcttc atagcgaggc atttgtggtc acggcttcct actcagaatc
    14641 ctgccgttgg gttcctgcgc ggcagccatc ggaagcgctg acgcacagac catgaaaagg
    14701 atgagcggaa tcaatgctgt cttacgcaag gtcttgccct cgccgattct gcgatcgctg
    14761 ccaacgattg gtcgccgtag ccctcgttgc agctttgccg cggcacggcc agagtattcg
    14821 acgggcggct cttttttctt cttaccacta tttccggtgc agatacgcaa tcaactccgc
    14881 gatctgtatg tcactcaaac gtccgttgaa ggccggcatc gtccgttttc cgtagacgat
    14941 cacctggcgc actgccgcat ccgttgccgg catcgtgccg tcggggagat tttgatggga
    15001 gaagagaccc tgcagcttcg gcggcacctt cttgaggccg aggtcgtttt cctcgtgaca
    15061 ctcgaagcaa tgaatctcat acaggctttt cccagcttgt tcggtttcgg ttaggggttt
    15121 tgagcagctg gtggcccaga acaatggtat ggtcgcgaca ataaggccga gactcgatcc
    15181 caggactgac cgcccaataa gcctgcgacg ccgtgttctc agactcatct gccagcctgc
    15241 agagccagag ccgcgactgc attggcgacc tcgctcgggc tccatccgct gccgccccac
    15301 tgctgcgcta ccttgttgtc cttgtcgaga agggtggttt gcatcgtgtg ggtgatctgg
    15361 ttgtcttctt ccgtgtactg cagaccgaag gcctgagcga gcttcttcag atcgccgggc
    15421 gtcgtgtccg caaactccca atgggcgaag gcagaagcgt tgtcgtccag ataggccatt
    15481 ccgtactggc gcagtaccgg tggttgatcg tagttcggat cgaggctcac gctgatcagg
    15541 tgactggccg cgtacgctgc cggcatcttc gcaagaaggt cgttcacctt tgcaaaatga
    15601 ctcgtgatca aagggcaggc cgtgggcatc gggcagcgtg tgtagatgaa ggtgatcaga
    15661 acggccttgc cgcgatagtc ctggaggtgg atcagcttgc catcctgatt gaccaggggc
    15721 acatcgggaa ccgtttctcc aaccatcaac gcatgcgggg gaagcatcgt ggggggcagc
    15781 ttccgtccct tctcgctggt gatgacgaca cgatcgagca gataattgtc cgaatccgat
    15841 ggcaccagaa catccgccgt tatttcgtcc cccggcttca gcttctgcac gtcgactgga
    15901 ttcttcacct tgtagaccat cgtcatcgcc ggcatgaagc cggggatgtc tccgtgcctg
    15961 atggtgatct cgctggtcgc agtgctggtg gccaggacca tcccctggag acgatactgc
    16021 ttctccgtga cggccgccgg atgcagttcc acaccggcgg gattattgct ttctttgcgg
    16081 cacccggaag gcaaaagcat catcgccgca ccaacgacca gagcggcgac accgaagcgc
    16141 gaaatcaaca acatgcagtt tcctcaatca cgaaatattg cgtgcgttaa ttcacctgcg
    16201 ctcatggggc ggcgaacttc tctggtgagg catcgaaaac ctgcttgtgt tcgcgctcgc
    16261 agaagtagta cgtctttccc ttgtaggtcg aggacagaga acgatccacg tccattccgc
    16321 agatggggca gactggatga ttcgcactct gcctgatggg cccggcatac tcagcgttgg
    16381 aagaaggatt cagccagccc cggccctgat attcaagggc gtaggcggtc cggctcgcta
    16441 cttcccggcc gaaatagcgc tcgaccaccc gcaaggcgag gtcaatgccg gaagtgaggc
    16501 cgcctgcggt cgccagattg cccgtttcca cgaaccgagc cccgcgttca acatgaatgt
    16561 ccggatactg cagcgcgagg tcgacgtaag cgccgtgatg ggtggtggca ggcttgccat
    16621 tcagcaagcc agtgctcgca agcacaaagg cgccggtgca gacggacatg gtgacatccg
    16681 tactcctggt ggcattgcga atccacgctt tggtcgcttc gctggcagga ctttgtgcgg
    16741 gaataacaat gaccttgggt ggcggggcat cggcaatggt gtaggtgggt gtgatcttca
    16801 atccaccgct cgcaacaatg ggagtcgccg tctccgccac agtgtagaga ctgaaggcat
    16861 caaacttacg gccgggaacg gcggcctgct cgaagacttc ccacggacca caaaagtcaa
    16921 taacaacggc gccttgagag atcaggaagg cgaccggtat ggacgcgttg gcgggagggg
    16981 tgagcgggtt gagcgtcgcg cttcccgcgg ctgcgtggaa tgtttcagca gtcgcgccag
    17041 gtaaggccaa cgagccaaca gcagttaagc cgagggctgt ggtgcgttga aggagttctc
    17101 tgcgattcat tgagttaacc tctgcaatcc tgttgcgacg agcccaggcc agaagcgcct
    17161 ctatgtgttt cgtgctctgg cagcggcata ttgaccgctc acaaagtccc agttcacgac
    17221 gtgaaagaat gcggcaacat actccggccg tcggttctga tacttcaggt agtaagcgtg
    17281 ctcccacaca tctaagccga ggatcggttt acggccgctc gtgagggggc tgtcctgatt
    17341 ggcggtcgtt tcaatttgca gcttgccgtc gccgcccagc gtgagccacg cccagccgct
    17401 gccaaagaca cccgcggctg ccttcgaaaa ccgctcctga aactgatcga agctgccgaa
    17461 ctgtgcgtcc atgtccttcg caagttcggc cttcgggcgg tcaccgccgc tgcgggacag
    17521 agtcggccac cagaaggagt gattggcatc gcctcctccc tggttacgga tcgccgtgcg
    17581 gatcgattcg ggcacggtat cgagatgggt cagcagttcc tcgacgctct tgcctgccac
    17641 ctcgggatgg ccggccacgg ccgcattcag gtgctctaca tagctggcat gatgcttgtc
    17701 gtgatgcagg tgcatcgtct gcgcgtcgat gtacggctcg agggcgtcgt acgtataggg
    17761 cagggacggc agaatatagg gcccgcctgg cgctggtcct gatgcctgag cgagcgcgta
    17821 ggggttaagg cttgggccgg atattgcgag tccggccccg gaagcaagac ccttcaggac
    17881 tgaacgtcga ttcataatcg tctcctttca agcgaagcgc gaaattcgtc tgcaatgcgg
    17941 aagttcaaag gtcgcattcg gcggatgctc tcatgatgga gctgactccc cgcccgtgcc
    18001 tgggttgtgc gaccatcggt actctcagaa cgtagcgatg aggttgactg acacggaacg
    18061 gccgcgtccg gtaaccggag tcatgctgcc ggtggccccg tagaggtcaa tgttattgcc
    18121 gccaagaggc agttcgtaaa aacgattgaa gaggttgtcc gtgcggaacc caccctgtac
    18181 atatttagtt ttgtaaacgg cgtgaacatc gacgagcgca aatcccggag tgcgcagctc
    18241 catgcgattc gggtcgaggt gcgacttccg gtcgaccatc tccgagccaa agccggcagt
    18301 gaagctcttc gtatgttcat cgaaagcaag gcgcagattc agcggcatca tctggtaaag
    18361 cggggtaccg ctgtcggtac gcgtgccatg gagccatgct ccgctggcgg tcagcgtgcc
    18421 ggatccggag ctgtcgttac cccacaaggt gactaaaccc gagagatcgc cgccgtagat
    18481 gcgagcgtcg tgattcgcaa agcgcagcag cggcgttccc atcatggatc ccatcatcgg
    18541 catcgaggca acgttcacgt cgatgtagtc gtgaatgtaa ttgaggtgag gtgtgagctt
    18601 gacttcccac ggcctcggtg tctgcccgtg cagcaacatg gtgccgctga caacattccc
    18661 agtctccggc ttcagactga tgtcgccgta ataggagttg ccgtcgccaa accagccgat
    18721 catgctggcc gccatcatgc cggttgacca cgtgtaccgc tcgtagagat taggtgagcg
    18781 gttctttcgc gcatagccga gctcgaaggc tgcgtgccgg ttggcatcgt aacgacccat
    18841 ggcagtcaag tcgaagttca cgtcggtccg ggaatgattc tcagcgttga aggcagcggc
    18901 gtcggcagca tacatcgccg agtagccgtg cacatcgccg gcgtcggacc acaccgtatc
    18961 gttgcgcgct ccgaacagag tggaccagtg cgggttccaa cggctgaata tttcggcata
    19021 cgttcccagt cgggtacggc gaccattgtt gatgttgata aaggtgttcg gccccatcat
    19081 gggggccatt cctgcgaccg gcggccacca gtcatcgagg cgcaggcggt gcagctcgtt
    19141 gccagcgcgg acggtgtggc gagcggaaag cgcggtctcg tagcgaacca gatagcccag
    19201 gtcgcggccg tgggtattca tcggcatcga cgcatccgcg ccgtccacag cgagcttatc
    19261 ctgcagaaaa ttcatctcgt gcgtggtgtt ctgccaatag aagtgggcat cgagcgagcc
    19321 ggtggcaaag gttctgcggt agcgcagatt cagcgatgtg gcattgttgt gagtcaggtc
    19381 catgaaggcg ttcacaaagc cttcgaaagg cacatggtgg aagctgccgg tcgcgacgaa
    19441 gtagttgtgc ccgccatgcg cccccagagt caccgcgtgg tcggtgctct gcgcgtaggt
    19501 tgacgtgatc ttgcggcctg caccgtcggc atagtcgccg gttgtcgtga atgcaccgac
    19561 atacccgagg ctgaaatgtt cgctggcaat tgcctcgctg agagatccgc cccagttatc
    19621 gccgtttgag cgatagaagc cgctgaaagt tccctcctcg tgcactctgc ctccgggctc
    19681 tgcaaaaaca ggggcgggag tctcgaccgc gatggtaccg ccgaggctgt ctccaccaag
    19741 actcaccgga gtgatcccag ccagcacggt cacctgcgct gcctgaggtg gggcaacata
    19801 gctgagggtc gggttcatgt ggtttggaca ggcgctggag atggtcatgc catccaccac
    19861 gattttggtt cgctcatcgc ccaggccatg caggaagggg atcgtggcca gctcaccgtt
    19921 gccatgcagg ctgacgcccg gtacgtcggc cagcatatcg gcgctgttat gactttggga
    19981 agaatcggag ggctgcacct ggagaatcga agcggcaatg gagcttggtt gtccttcaac
    20041 ctgaacctcg gcggcgacga caggaatgct gagagagatg tcttcagaaa tttctttgcc
    20101 ggctgccacg gtcagagagt ggagagtctg accggcaaac cccgaggcag tgacggtgag
    20161 ttcgtaattg cctgccggga cagagacaaa ggaaaagtga cccaggtgat cggagatttg
    20221 ggtttcgcga aagctcgtgg cggtggcgcg caattccagg cgggctccgc tgatcagagc
    20281 acctgaggga tccctgagaa ctccggcgat gctgccggac gaagaggttg tcaatgcggc
    20341 tgattgttgc gcatgaacga ccggggcagg cgcgatcatg caggcggtcg ccacaatagc
    20401 cgtccagatt ccactttgca ttcggttttc ctttttggtt cgctgcacaa acagcggttg
    20461 taaaaccagg ccttgtccat caacaaaact gcgccccgcg gcaagcgcag cggtttgcaa
    20521 tacctaaggg tcttctcaat ggggcagggt ggagccggcg caaaggtgct cgcgctagtc
    20581 cccatcatgt caaaggaagg cagctatctc tgtgtggcaa attgccgcag cggggccgtc
    20641 aaaggccggc gccagtcaca actcagcggt tcggaggaaa tttcgagagc ttgcgctgca
    20701 gcgagcgacg gtgaattcca agcgcccgcg cagcctgaga gatgttgcct ccacaatccg
    20761 cgatgacacg ctgcatgtgc tcccactcca cccttgccag gctgggggcc tccgcggggg
    20821 aatcgggaat cgcctcgtgc ggcgccccga gtttttcgta agcggccaga atctgatcgg
    20881 catcggcagg cttgctgagg tagtagtccg cgccgcgctt catggcagaa atcgttgtgg
    20941 ggatgctgcc ataacctgtc agcacgatga ttcgcatgga ggtattcgca gcgcgaagtt
    21001 cttccacaac gctcaggccg tccatgcccg gcatcctgag gtccaccaac accaggtcag
    21061 ggatcgactc gcgcgccatc gcgagggctt ggctgccatt cgcagccgcg gtcgcctccc
    21121 agttccgttg tgccagcgct cggcacaatc tggttcggaa cacctcatcg tcatcgacga
    21181 ccagtacgcg acgggttctc tcaggttgag acacgatggc tctgatcctg ttttgagaaa
    21241 tgaaccggga gtgtgagcgt ggctgtcgtt ccgttgccca gggcagactg gtacctcaac
    21301 tcgccgccga ggcgctgcgc gaaggtgcgg accaggaatg tacccagtcc catgcccttt
    21361 cccggttcct tggttgtaaa gaacggttcg gcaatcctgc gcagagtctc gttgctcatg
    21421 ccgctgccat ggtcacacac ggagaacaca atctctgagc ctcggctttg cgcccgcagc
    21481 gtgatccgcg cctctgaacc cgcgtcgagc gcgttgctca gcagagctgt gagcgactgg
    21541 accgtcgccc gcaccggcag gaagagatcg gttggcgcgc catctgcgtc gatggagatc
    21601 tgcgagcgtc gcggttcagg gatctcccgc agcgtctggc gaagaagttc gccaatctgg
    21661 atcgcccgcg gcgactcgcc gaccggctcc gccccatccg cgctcatgcg ttgcaggatg
    21721 tgctcgcacc gctcaacttc tgagcggatg agcttcgcat cgtcgcacac tgcgtcgctg
    21781 ttctccaggg tggaggcgta aagctccagt tcgcgagcca ccacagcgat ggttccgaga
    21841 ggcgttccca actcatgtgc ggcgccggcc gccagagtga ccagcgaagt cagcctctcc
    21901 tgccgagcca catgctcctg gagcgccagc acctcttgtt cgcggagacg tagcgcattc
    21961 gacactctgc tggtgaaaaa gctgatcaga gccgccgcga tgacaaaagc gatccacatt
    22021 cctgccagat gcgaagaaag cccgggctct ccggaatgca ggtgtgaccc cttgagcgag
    22081 acgtggagga cgaacagaag accaaagcaa acagatgcga gtacaccgag cccccacacc
    22141 cattgcttct tgagcacaac cgccgcgagg gtgatctgga caagatacaa gaggctgaac
    22201 ggattcatgg gaccacccgt cagcccaagc acagcagtca ggcagacggt atcgagcaga
    22261 aagataaaac ccaggatcgt ctgcggggcg cgaaccgatc tcgacgccac acgatgcagc
    22321 caaacgtttg ttgccagtac ggagacgagt ggcactgtgg tccacagcag cgcggggtag
    22381 aagccgagga aatacgcgac ccccccgagg gtggctcctc ctcccagaac catggcgtag
    22441 cgcagccgta cgacccatgg cagagccaga ttcggagcgg cgtcattttc cagcttctgc
    22501 agtgcaggca tggcagtggg catcatcctg atgatatttc ggcccggaac cattagcacc
    22561 ctgcgacaat ttgtcgcact cggctgtctg gttggacagc cgtttctgca gttccatcgg
    22621 ttcgactcgc atatgagccc attgaaaaga ttggggctac cagcgggtag ccccttgtaa
    22681 ttgatggatg gaatgaggcg gcagcatacc acgagttgga agatccacga attgcataag
    22741 cgcgcattgt tcaagaacaa tgagattcgg aagattccca gttctactca tcacacaaac
    22801 ggaaatgcga ccgctcgaca gccatcgcca gggcgtcgac atggttgtga ctgggcgatc
    22861 ttcgtaaatc ccggagccga cagaccgccg gtcataccgc agcgtgacgt tggtaacggt
    22921 gctacttttt atgaattggt attacgatgc catgcaggag cttgcatggg agacctgagc
    22981 aggatcggta tcgccctcga ctcggacctg ctccgccgtt tcgataaatc gatcgcgcgg
    23041 cgcggctata ccaatcgctc ggaagccttc cgcgacctga tccgggatcg actggtaggc
    23101 gagcaaacgg ccatgcccga tgcgacggtt gtgggtacgg tcacgctgat ctacgaccac
    23161 cacgcacata gcgtgggtga gaagctcacc gaaatccagc acgagcacca tcaattgatc
    23221 gtctcaacca gccacgctca tctggatcac gattcttgtc tggaagtgct gattgtgcac
    23281 ggccgctcgt cgcaggtgga gcggctggcg gatcggctca tcgggctgaa gggcgtgcag
    23341 cacggccgcc tggtcatgac ggctccgcag catccgctcg ggccgccgga acacaaggtg
    23401 cacaagcacg caaaataggt cgcgggaagg cagaagatgg gattgtgcag gcgcactggt
    23461 agcacgatat ttagattcgt attactgctg tttttgtctt ccttaactgg agttgtcccc
    23521 cttcacgctg ccgcgcagga gaagacatcg gtcaccgcga atgacggcct tcctgcgcct
    23581 cagctatcgg gaaatgtcgt tgacgcgtca ggcgcggtca tcgctggaac taccgtacag
    23641 gttcgaagcg cgaacggcgc tgttcttaga acgatgcaaa cggacaggga cggctgcttc
    23701 aatatctacg gacttcccgc aggccattat caaatcgtta tctctcatcc cgactttgaa
    23761 accagggaaa tgcccgtcac cattacgacc gcccgaactg cggcgactct gggcatcact
    23821 ctgacgctga gcgcggtcag caccaccatc aacgtgcagg gccgggcgga cgatctaatc
    23881 ggaattgccg attccgccac cctgggaacg gtgggtgcca gggaaatcga ggatcgcccg
    23941 attcttcgtt caggagaaat tctcgagacg attcccggtg tgatcatcac gcagcacgcg
    24001 ggcggtggca aagccaatca gtattttctc cgcggcttca acctcgacca tggaacggac
    24061 ttcgctgttt tcaccgacgg tgtgccgttg aacctgccct cacatgccca cggtgaggga
    24121 tatgcggaca tgaacaccgt catcccggag ttcgtaaagc gcgtggacta tgagaaaggt
    24181 ccttatttcg ccgacgtcgg caactacggt tcagccggat cggcgcacct ggaattcttc
    24241 aaaatcctgc ccggtaattt ctttcgcctt gagggcggga tgtttggata tgggcggatg
    24301 cttttcggtg catcgcaaaa actcggtcca ggcaatttac tttacggcgg cgaagcgtac
    24361 cacgacgacg gcccatggac ccatccggac aattactaca aattcaacgg ccttctcacc
    24421 tggagccagg gcggagacga ccatgggttc agcatcacgg ctcgcggcta tcacggaacc
    24481 tggcattcga gcgatcaaat ccccgtgccc gccgtaccgc ttgttggtct cttcggatcg
    24541 ctgaacccaa ccgacggcgg acactcccgg cgttacagcc tgcaggggga atggcatcgg
    24601 ctggacgcgc attccatgac gagagttgtg atctacggct tttattacga tctcgatctg
    24661 ttctcggact ttacgtatta cctgaccgat cccagtcgcg gtgaccaatt cgaacagcag
    24721 gaccggcgct gggtggccgg gctggacgcc cgccacacaa tcttcagcca gtggctcggc
    24781 cgcagagtgg agaacacctt cggcgtgcag attcgtaacg actgggtcca caacgggctc
    24841 taccagtccg aacatcgagt gcgcgtggac aaaacggatg ccgataccgg taccactctg
    24901 cccgccacga cccaggcgga tcgctttacg gatacccaac ccggtttcta tgcagaaaac
    24961 accatccact gggcagacaa atttcgctcg gacgcagccc tgcgcggcga tctcgaatct
    25021 ttcgacgtta caagcctggt gacatccgca aattccggcg ccgccagtaa gtggctgccc
    25081 agtccgaagc tcagtctggt cttcggaccg tgggccacca cagagttcta tgcgcagggt
    25141 ggtttcagct ttcacagcaa cgacgggcgg gggactacgc aaacaataga accgatctcc
    25201 ggagagaatc cttaccctaa tacgcccgcc gacaaaatcc cgcctcttat cccgaccaaa
    25261 ggcggagaga tcggtgtgcg cacggcggct gtgccgcgtc tgcaaagcac gttttcactt
    25321 tggtatctcc acagcgcttc cgaattacag cagtcgggag acaccggagg cacgactgcg
    25381 tcacgacagc cgagcaaccg ctacggcatc gaattagcga actactacac acctgcggaa
    25441 cactgggccg ttgacctgga cctggcacac tccaaagcgc tttttaccga aatcgatggc
    25501 gatgatgcgg cgccgggtag tcccggaggc aagcgtgtgc cggaagcggc tgggctggtg
    25561 attgcctcgg ggatcacgct gcacgattac cgcggattct cgtcgagttt gcgcctccga
    25621 tactttggcg cgcgcgatct gacgtccgat ggactctttc gctcaaaggc aacagctttg
    25681 gtcaatggac aggttggata tcaaattggc gagacatggc gcgtttcagc cgaatttctc
    25741 aatctgctga ac
//
LOCUS       DASZYN010000331        17473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_269752, whole genome shotgun sequence.
ACCESSION   DASZYN010000331 DASZYN010000000
VERSION     DASZYN010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 17473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17473
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269752"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VHE33_08560"
     CDS             <1..385
                     /locus_tag="VHE33_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97546.1"
                     /translation="HLQSAPDSCSVAASLQQSERWLPLSTQHALELIANALPTAPATF
                     SEQQVADLFQTASGVHLQDLDHLVDMGLLSTIARNRYQLHPVIAAYVRLKSEEEATTR
                     LALHSWACEISEPFSREKERKVCDA"
     gene            1548..2648
                     /locus_tag="VHE33_08565"
     CDS             1548..2648
                     /locus_tag="VHE33_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97547.1"
                     /translation="MGLQQWLPVSPGAPGSGQGAGGVAANATASLDASPSCWPTDTNC
                     LITSIASHIAAGIQAAFQPLTEGVLKTPADILYQTPLLTNETEAQNQVIHTLNTFFIT
                     VVDLAFACLLVIAGYNVIIGRHLLMPSSTLVELLPRAVLVMLAVHWNLFFLSLFIAFE
                     NALSLAVFQFAGAQMLTNIIAGLLTFQGVSLLTFLLLVVLGILSLLLLIQMVMRIALV
                     ALSLVLAPLGLGCFFLPQTIRWGRLWLAILSSSVMVQFLQVVALGLGGVFLSALAQTS
                     FFHLDQSLVTALLAIGTMGLVLKIPGMLQTWALHPMMDAGGRATSQADTMETSPSSGP
                     SSATSSVESSEGAAAAATTDTTVAAEGAMLLL"
     gene            2673..3980
                     /locus_tag="VHE33_08570"
     CDS             2673..3980
                     /locus_tag="VHE33_08570"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HVT97548.1"
                     /translation="MMNTPLWMKWLLGIGVLGILGFALLVGVVTQVAGAANASSFSAC
                     PGTQEPTPPTTSHATATITSGDAATCYPSSPYGAAVVSWAKKMADALYVNPACGTARG
                     GACNDTWYTSAFPQPVIDYGQRWCQAHGDCADWANGSYQCVSFVRGAYSQVYPMTLTN
                     DAFGLWATYRSVPGWQEIPSLATSDPSQRALPEPGDVMVFRDVSIGHVAIVMAVQAPG
                     AHTNGWIAFANANSSSAYDRMPLRPDLTVDTSAWAGSGMQFEVWGYIRPQPALAHRVV
                     RVNQLDPAQYASQGEYETWAYSACSAAAMTEVLNAYGFHLRIHDVLVIESARGDIDPL
                     LGLTHDGGIADTLAQFGLQTRWGDHWSLAQVVAVANGGLPVIVGWPPDRYDGGHLVVV
                     TGGDLARGTITLVDSSAWNRQVISITQFQQWWAGFAAEAIPQT"
     gene            4068..4418
                     /locus_tag="VHE33_08575"
     CDS             4068..4418
                     /locus_tag="VHE33_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97549.1"
                     /translation="MVPWNSVCVTLGALFLRFAAMQGSATDPNANAISAFFGRLTADL
                     TVFALAMAGFFFALSALFYMASGATQNERTRQHAISALYAALGGLALALLAGTVALLI
                     NNAATGASAGGPAN"
     gene            4435..4824
                     /locus_tag="VHE33_08580"
     CDS             4435..4824
                     /locus_tag="VHE33_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97550.1"
                     /translation="MPTSSHRTHLVPTHIKTPETLLTLAGVSLSVRQFLLLLTGVSLS
                     YRLWLALTPLAFLPAGTILQGVLAALPLGAALAFAFVFIAGRALDSWCVITIRYVLRP
                     RQFVWRSVRFAEPMWGTTTQEEHYEAR"
     gene            4811..7588
                     /locus_tag="VHE33_08585"
     CDS             4811..7588
                     /locus_tag="VHE33_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97551.1"
                     /translation="MKPGKPSRRASVQQYFVQAREILNDVLCLFASHSPTKRTYCSVL
                     EVSTINFLLKSSDEQDALVERYRTILKALTFPVQIIVRNQRLDLRPYLAQVQAQVPRT
                     PGCDADLASKSPDDLHEDIASPVGERDVAPAWGELAAGLETYVQQVGSQRTLVERHCY
                     LVIPAPDPLLSSRRFSVRPKRRASQEAIQARCLQELAIRCETVQQQLASLGLRTRRVR
                     GEDLARLYQSFLTPERAYLHPLRSANLAAVGHLPRVLRSSTAPTPDRASDLPNGNEPD
                     RSPEEDDAQVPSHRRRWRHHPVSSEAARAPADFLRLADLLAPASLVETRDCLRIDEEY
                     VRGLAVVAFPREVSSGGWLAPLLMLDEMVDIVFHLRPQPQAAMMRMLKRRRVGYASAR
                     TFNHKQGRLDDPDTTVAQNDVTRLMSALASGEERLFELSFLLILRAPSKAALNERTER
                     VLALLQTLFLDAVAHPVTFEHALAFRSSLPACRDEVGRTITLDSASVATTFPFMSNSL
                     TMPGGTFLGLTSTGEPILLNPWNTALENPHAFVGGVTGGGKSYLGKLWLERSLLMSGA
                     SGERYAVIDPDGEYLPLAAALGGSVVRIAPGSHHHLNPFDLIPPGCDLPTYLAGVQRS
                     DRLAEKIQDLQSLLDLMLADAGTTLSAREKALLDRALYEVYRRASISADPQTHFHQPP
                     LLRDLAEVLRSGACGPDEWDLGLRLSRYTEGSLAGLFSSQTNVNLDTHLLIWDTRDMR
                     GDMRPIGIFLIADAIWTQAIYQSHIRRCLSIDEAASLIEHPEGGRFLANLSRRARKRY
                     LRLVIMTQSPESFVEDPSGSVVASNAAIKILKKQDRTSIRAVAQRFGLTSGEEQRLLL
                     FGVQEALLFAGDRRVLLSVQASPQEHRLITTNPMERAQQAAPAVPDSGGTRRATSPTG
                     HSIQEAEGEEC"
     gene            8143..10518
                     /locus_tag="VHE33_08590"
     CDS             8143..10518
                     /locus_tag="VHE33_08590"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HVT97552.1"
                     /translation="MVLLVPVVLLLLGLYLFHGLVPTWLVQAALDLLRGHPPHLSTAE
                     TVAVVLGAGAGLIVLLGGAYLAILLVSRFESPRIYDQRLVAEKTARPAYRVRVRVWVF
                     GPAPHTRGAATSGSRIDRLVRAGVRAWKRWEAWMSHPPHLHDIIHQLSNQWQAHRVFV
                     RDRRARHQERTAILRQIVGAYRQYHLAAGGYFLPRPLSARQARRLLTPPGRRGFRRVG
                     WAADLARSPHYLSVADLAALWHLPQADDLADLVYVPHTTMRTLLAPPILATGQGYLLG
                     TSTHAGHQLPVFLPFACLSQNLLAAASTGKGKSTLFYHLSRAFAHGRLSHEAGVPNGM
                     LLIDPHGDLVHQVAGALPSALADDVVVIDLADRDYPLAWNPLDMAGTGKDRDKVIDHL
                     IQVIEALWPTSYGPRTESFLEYSCKTLAEANGRLLADDPLTGPDRQYTLLDVVPLLRR
                     ASFRHRVLEQVDDPYLLTWWQQYYELLDARQQADFTSSLITKLAKFSSSRIVSRILGQ
                     PRSTIDLGDLIRQNTIILFSCAAGEIGADMAALFGSLFVGFFQAALQEQASLAVSDRH
                     RFLVLIDEFQALAGINYQSMLAELRKYGGSFALATQSLAYLDRFERTLRATVLANTEH
                     LFAFAMADEDARLLRLPGIDPEDIVHLAAYTCYARLSLQGERLPLFSLHLQAPEPEDR
                     SLRQTIVDRCRARYGRPVGEVERILRDGLVRQSVPGDLGKAHGWDLEGVETVAEAVER
                     IKKKRKRGSGASKKAAASDLQGTAGAVPSHLLYEEEEASPDAAEDTEKGGA"
     gene            10518..12557
                     /locus_tag="VHE33_08595"
     CDS             10518..12557
                     /locus_tag="VHE33_08595"
                     /inference="COORDINATES: protein motif:HMM:NF025186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HVT97553.1"
                     /translation="MPPSAHALALTLEEGALRLLLWLDHYPLQRAQDLILAAAPWEKR
                     TTVYRHLADLERAHLIEELYAGITAKKRLYHLSPLGCAVCDYLAAQEDHPQAEKQARW
                     ERWTRQDVGAVVREERGKLVRLLPRLPLWVLLQDTIEGLVRNADRALARQGRHAQLIQ
                     WNWMREYRHPFVSREQTLRVQAEAALTFCLRFPQAPGAPPSSSPLATEEVWYTMMVLH
                     CPLDEPRLMRQRLDRLLRWRESAERMGVYSHMPPVVILATTERQAEWWHQLAAQTAAR
                     LRVDPLVGAVAVLPPEPVSCWHLPFRRLDTREMCHLHNLLSPLPVPALPELLASRGDA
                     RRSKAARETRAARTVLTLPLPSRLRGRSFLPGKARVPVRRGSPPQQRTPGDYCALSVA
                     LTARQWEILRLCFAHPLLSREDLTHFLLLSRTTTTLLLGDLARLGYLASTSTLVGERW
                     QVSESGLRLLARLVGCQVHRFIRFPVEAGHPLVQRGVRGLMHQIRHTAGVYAFFAQVS
                     EALAHQPDTRLLWWETGVLSERHFAFRERIYRFRPDAFASVQIGQRPMRFWLEWDRGT
                     MTAKNYQVKFTTYAMYLASREWARSSPLLPALLCVAPDIGQESQLTKAARRCVLQAPS
                     GWCVYSTTASLLMTQGVLGPIWRLVTAQEPTEPPAALERQRLFTAEAPDRGSEPL"
     gene            12732..13121
                     /locus_tag="VHE33_08600"
     CDS             12732..13121
                     /locus_tag="VHE33_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVT97554.1"
                     /translation="MRKRSDDGQPVPGPALAGHVIQRYEAQHAWVIDHLLLQGSVTEY
                     QCLGLLLERANTCVPYAAFLRRGIMTSTGNRKQDRGRLAALISRLRSRLWGCGFEIAN
                     VWNVGYLLLADPPAYSDRTRGRPEELS"
     gene            13190..14125
                     /locus_tag="VHE33_08605"
     CDS             13190..14125
                     /locus_tag="VHE33_08605"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT97555.1"
                     /translation="MQQRPTVSFIGLGQMGQPMARHLLQGLQGKRDLFVFDAQAERMT
                     ALTALGAHGGTCLDDVVPRGGVVFAMLPDDRSLLQVALTDGGILTHLGTGGTLIVCSS
                     VSPDVSAQLARLSHQQHCAYLAAPALGGPEAAARGDLSFVLAGPRLAKRQVMPLFACM
                     GQRLYDLGERVEAANVMGLAWHLLRASLIEALGEAAALIDAYHLNRSRLADLLQAPSL
                     WQQTADAVRRIGARDFSEPQLSVQAGLRMLDLALHLGQQRDLDLPYADVAYGHLLAAL
                     DAGREQEDWSVLSDFARQGATDWFTREEDADAPLA"
     gene            14109..15611
                     /locus_tag="VHE33_08610"
     CDS             14109..15611
                     /locus_tag="VHE33_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVT97556.1"
                     /translation="MPPWPETPWQGMTARREQHLPMHRLALDAEHFKRVGQHVLEQAR
                     TFLYDERPIYQPLSREERERWQQAPLPAQGLDPAHLLSELQEVSQHATFGINHRRSYA
                     YVGGGVNEWGVLASLLAAALNPNCIGGDQAITYMELATIRWLAELSGFALPPHGGGVL
                     TTGSSQATLLALAAARQRLARRLGRDMRVHGLQGLSPLAIYVSDQTHASVHQQANVLG
                     LGRVCVIASDDEGRMRLDVLQETMERDERLGILPCCVVATVGTTNTGAIDPLEALAEV
                     CERHRLWLHVDGAYGGLGWVDERLRERYRGIERAHSLSLDGHKWLGVPYESGCLLVRE
                     QQALKEAFARPTPSYLKQVEDEHPHFSDLSAQVSRAPRALVLWAVLRSLGYAGVRSLV
                     MRHRDLATLLAAGLVHLPGMRLLAPVELSTVCFRYEPAWAQGDEEHIRTCNQHLLRDL
                     ESTAFLSTTELHGQWALRACVLHPRTDWDDIRLFLDALPPALARQEAGKP"
     gene            16152..17021
                     /gene="aroF"
                     /locus_tag="VHE33_08615"
     CDS             16152..17021
                     /gene="aroF"
                     /locus_tag="VHE33_08615"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HVT97557.1"
                     /translation="MHAYPSSHHPLLGSRSVHPTRTIVTVPAACVDGGQVALGDGHLV
                     MMAGPCTVESERQLMTTAEWVRRQGAVILRGGAFKPSTSPYGFRGLGEAGLKLLAKAR
                     DAFGLAIITEVMTPTDVELVCAYADLLQIGTRNMQNYMLLDAVGKTHTPVVLKRGMAA
                     TIEEWLLAAEYILAQGNDQVILCERGIRTFEPMTRNTMDLSAIPVVKQLSHLPIIADP
                     SQGTGHRELVTPLSLAAIAAGADALLIEVHPHPEEALKDGAQSLTLEQFQALMPQAQA
                     VAHAVGRSLMGPR"
     gene            17136..>17473
                     /locus_tag="VHE33_08620"
     CDS             17136..>17473
                     /locus_tag="VHE33_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97558.1"
                     /translation="MDDAHLGETLLHLALAMDSARHAYWRGTPAHDLLQRDTQVYQHL
                     AQERPRGMELMIDLYHCDPARLRDPQHLHAFVTALTALLSRYQPGESLITRVGPHAQR
                     RGSTLVQWHDT"
BASE COUNT         3057 a         5608 c         5234 g         3574 t
ORIGIN      
        1 acatctccag agtgcccctg attcctgctc agtggcggca tcgctccaac aaagtgaaag
       61 gtggcttccc ctttccacac aacacgctct ggaacttatt gccaacgcgc tccccaccgc
      121 acctgcaacc ttttcggaac aacaggtcgc ggacctcttt caaaccgcct ctggagttca
      181 tttgcaggat ctggaccatc tggttgatat ggggttgctc agcacaatcg cccgcaatcg
      241 ttaccaactg cacccggtca ttgctgccta tgtccgtctc aaaagcgaag aagaggccac
      301 aacacgcctt gctcttcact cctgggcatg cgaaatatct gaaccattta gtagggagaa
      361 agaacgaaag gtctgcgatg cgtgacacct cctttccctt tcgacacagg ggtctgcctt
      421 cgtcacatct gctggccacc cgcttgcacg tgtgctcgcc agaaaccgct tttcggttgt
      481 atccaaccgt tgaaatgcgt cattggtgcc agcagcagcc aggcatcctc ttcccatttg
      541 ggtggctgcg tcttccaggg gaactccccc ggcgctatgt tggtggtttg gtcaccggcg
      601 cgacgctctt tctgttcgtc ccctcatggc tgtatgagcg tcaccggcac ctcttgctga
      661 cgccgtgtgt accacactgc tccctgagga gcacggctga gagacacgct gaatatagat
      721 gggtcatctc cgatgacgcg atctgccagc gagaagcatg tgcgcggcag gtgcagtatt
      781 ggatgccgtt cctcgccgct cgccaggaac ccccgacagc cggctggcgt tggttctatc
      841 gagactgagt tgctgtctcc acctgcgtct ggcggcaaaa acgagggtgg tctcttcctc
      901 ccaaacagaa gaggaagaga ccaccctttg ctggaacctg gcgcacattc gcccaggacc
      961 atcactggcc gagtcgcctt gccctgttct ttctcactca ttccctccat cgctttgcgt
     1021 tcttctctcc ctttgtcttt cccctcacct atttgcgttt tttgctggac ttttgcgctt
     1081 tttcgcgcga aggtatgagg gaaaaagcgc aaaagcctgt ggaaaaacac ccaaacgttt
     1141 cccccctttt caccgctctt cccggtatag ttgttcctgg cagaagaaac gaccaggatg
     1201 ctgccccact cgtctgggag cagtatgcct tcttcaccac cttttctgcc atggatagtg
     1261 gactcgtcgt gcggaccgac gtgagtgcct tcgtgagccg atcatttgct gctcacgaag
     1321 gcactcacgt gatgtgcgcc ggggtagacc ggggccatgc agccgcgaaa gacagcgcat
     1381 cggctcttct ggacatgacc tggcatcggg ccagcaacag cacaaaggga tatctcatga
     1441 gacaacctgt tcctacagca accggattcg gacgtggcct ccgtcggttc ttgccttttc
     1501 gtttgggcat gcttttcctg ctgagcgtct gcctttttgg ggtacttgtg ggcctccagc
     1561 aatggctccc cgtttctcca ggggcacccg gatcaggcca gggcgccggt ggcgttgccg
     1621 ccaacgcgac cgcttcgctg gatgcttctc cctcctgctg gcccacagat acgaactgcc
     1681 tgataacgag tattgcgtcc cacatcgcgg cggggattca ggcggccttt caacccctga
     1741 ccgagggtgt gttgaaaacc ccggcagata tcctgtatca aacgcccttg ctgacgaatg
     1801 aaaccgaggc gcaaaatcag gtcattcata ccctcaacac ctttttcatc acggtggttg
     1861 acctggcctt cgcctgtttg ctggtgatcg ctggatataa cgtgatcatt ggtcgtcatc
     1921 tgctcatgcc ttcctcaacg ctggtggagc tgcttcccag agccgtgctg gtcatgctcg
     1981 cggttcattg gaatctcttt ttcttgagtc tctttatcgc gttcgagaac gcgctgagtc
     2041 tggccgtgtt tcagtttgcc ggggcgcaga tgctcaccaa tatcattgcg ggattgctca
     2101 ccttccaggg ggtgagcctg ctgacctttc tgctgctggt ggtgctgggg attctgtccc
     2161 ttctgctgct catccagatg gtgatgcgga tcgccctggt ggccctgagc ctggtgcttg
     2221 ctccactcgg tttgggctgt tttttcttgc cccaaacgat ccgctggggg cgtctctggc
     2281 tggcgatcct tagttcctcg gtgatggtgc aattccttca ggtcgtcgcg ctgggtctgg
     2341 gtggcgtgtt tctctccgcc ctggcccaaa cctccttttt ccatctcgac cagagtctgg
     2401 tcacggcgct gctggccatt ggcacgatgg gtctggttct caaaatccct gggatgctgc
     2461 aaacctgggc gcttcatccc atgatggatg ctggcggaag ggccacctcc caggcggata
     2521 cgatggagac gagtccatca tcaggtccat ccagcgcaac gagcagcgtc gagagcagcg
     2581 agggcgccgc cgctgcggcc accaccgaca caacagttgc ggcggaaggg gccatgctgt
     2641 tgctctagca gcaagcacag gaggaaacaa cgatgatgaa tacccctttg tggatgaaat
     2701 ggctgctggg catcggagtg ctgggtatac tgggctttgc cctgctggtc ggggttgtga
     2761 cccaggtcgc gggcgcggcc aatgcctctt ctttctcggc ctgtcctggc acccaggagc
     2821 ctacgccgcc cacgacgagc cacgccaccg cgaccatcac cagcggagac gctgccacct
     2881 gctatcccag cagtccgtat ggcgctgctg tggtcagttg ggccaaaaag atggcggatg
     2941 ccctgtatgt caatcctgcg tgtggaacag cgagaggcgg cgcctgtaat gatacctggt
     3001 acaccagtgc gtttccacag ccagtgatcg actacggaca acgctggtgc caagcgcacg
     3061 gcgactgtgc cgattgggcc aatgggtcgt atcagtgcgt ctcgttcgtg cgtggagcct
     3121 actcgcaggt ctatccgatg acgctcacca atgatgcctt tgggctgtgg gccacctacc
     3181 gctcggtacc gggatggcag gagatcccgt cgctggccac aagtgatcca agccaacgcg
     3241 cgctaccaga acccggcgat gtcatggtct ttcgcgatgt gagtatcggg cacgtcgcca
     3301 tcgtcatggc ggtccaggca ccaggtgctc acaccaacgg gtggatcgcc ttcgccaacg
     3361 ccaatagctc cagtgcgtat gatcgcatgc ccctgcggcc agatctgacg gtcgatacca
     3421 gtgcatgggc cggatcaggg atgcagtttg aggtctgggg atacatccgg cctcagccag
     3481 ccctggcgca tcgcgtggta cgtgtcaacc agcttgatcc cgcgcaatac gcctcccagg
     3541 gtgaatacga gacctgggcc tattcggcct gctctgccgc tgccatgacc gaagtgctca
     3601 atgcctatgg ctttcatctg cgcattcatg acgtgctggt gatcgaaagc gcgcgtggcg
     3661 acattgatcc cctcctgggg ttgactcatg acgggggcat tgccgataca ctggcccagt
     3721 ttggcctcca gaccaggtgg ggggatcact ggagtctggc gcaggtagtc gctgtcgcca
     3781 atggaggctt accagtgatt gtgggatggc ctcccgaccg ctacgacggt ggtcatctgg
     3841 tggtcgtaac cggcggcgac cttgcccgtg gcacgatcac gctggtcgat agctccgcct
     3901 ggaatcgcca ggtcatcagc atcacccagt ttcagcagtg gtgggccggg ttcgccgctg
     3961 aggcaatccc ccaaacttga ttggctcagt cctgttatcc attcatacgt ttgtccagac
     4021 ctcggtcctg ctcttgcgga ggccgattgt tcaaggagag atgccctatg gttccctgga
     4081 atagtgtgtg tgtcaccctt ggtgccctct tcctccgctt cgcggcgatg cagggttccg
     4141 ccaccgatcc caatgccaat gcgatcagtg cttttttcgg cagactcacc gccgatctga
     4201 ccgtgtttgc gctggccatg gctggctttt tcttcgcact ctcggccctg ttctatatgg
     4261 cctcgggagc cacgcagaat gagcgaaccc gccagcacgc catttcggcg ctttacgcgg
     4321 ccctgggagg attggcgctg gcgctgctgg caggcacggt cgccctgctc atcaataatg
     4381 ccgctaccgg cgcgtccgcc ggtggtccgg ccaattagca gaggagagat gcccatgccc
     4441 acgtccagcc accgaaccca cctggttccc acacacatta aaacgcccga aacgctgctc
     4501 accctggccg gagtatcgct ctcggtccgc cagtttttgc tgctgctgac cggggtgtcg
     4561 ctcagttatc gcctctggct cgccctgacg ccgctcgcat tcctgcccgc agggactatc
     4621 ctgcaaggcg tcctggccgc gctgccgttg ggggccgcgc tggcctttgc ttttgtcttc
     4681 attgctggcc gcgccctgga tagctggtgc gtgatcacga tccggtatgt cttgcgcccg
     4741 cgccagttcg tctggcgctc ggtgcgcttt gccgaaccca tgtgggggac cacaacgcag
     4801 gaggaacact atgaagccag gtaaaccgtc caggcgggcc agcgtacagc aatactttgt
     4861 ccaggctcgt gagatcctca atgatgtgct gtgtctgttc gcgtcacact cacccaccaa
     4921 acgcacgtat tgttcggtgc tggaggtctc caccatcaat ttcctgctca aatcgagtga
     4981 cgaacaggat gccctggtcg agcgctatcg caccatcctc aaagccctga cgttcccggt
     5041 gcaaatcatc gtgcgcaacc agcgattgga cctgcgcccc tatctcgccc aggttcaggc
     5101 ccaggttccc agaacgcccg gatgtgatgc cgatctcgcc tcgaaatccc ctgatgacct
     5161 gcacgaggac atcgcttcac cagtgggcga aagggatgtc gccccggcct ggggggaact
     5221 cgctgccggg ctggaaacct acgtccagca ggtgggaagc cagcggaccc tggtcgagcg
     5281 ccattgttat ctggtgatcc cggctcccga ccctctgctt tcctcacgac gcttctcggt
     5341 acgtcccaaa cgccgggcaa gccaggaagc gatccaggcc cgttgcctcc aggaactcgc
     5401 catccgttgt gagacggtgc aacagcaact cgcctcgtta ggcttgcgca cccgacgcgt
     5461 gcggggggag gatcttgccc ggctctatca gagctttttg accccagaac gtgcctatct
     5521 gcatcccttg cgaagcgcca atctcgcagc cgtgggccat ttgccccgtg tcctgcgatc
     5581 ctcgacggct cccacgccgg accgcgcttc ggatctgccg aacgggaacg agccggaccg
     5641 atctcctgaa gaggacgacg ctcaggtgcc ttctcatcgt cgtcgctggc gccatcatcc
     5701 tgtctcatca gaagcggctc gtgctccggc agattttctg cgcctggcgg atctgctggc
     5761 cccggccagt cttgtggaaa cgcgcgattg cctgcgtatt gatgaggaat atgtgcgcgg
     5821 acttgccgtc gtggccttcc cgcgtgaggt gtcttctggt ggctggctgg cgccgctgct
     5881 gatgctggat gagatggtgg acattgtctt ccacctgcgt cctcagccac aggcggcgat
     5941 gatgcgcatg ctcaagcgcc gacgcgtggg ctacgcgtcg gccagaacct tcaatcacaa
     6001 gcaaggacgc ctggatgacc ctgataccac agtggcccag aacgatgtga cccgactcat
     6061 gtcggcgctg gccagcggag aagagcgcct gtttgaactc agctttctgc tgatcttacg
     6121 cgctcccagc aaagcggcgc tcaatgagcg cacggagcgt gtgctggcct tgcttcaaac
     6181 gctcttttta gatgcagtgg cccatcccgt gacctttgaa cacgcgctgg cttttcgctc
     6241 ctcgctcccg gcgtgtcgag atgaagtcgg acgcaccatc acgcttgatt cggccagtgt
     6301 ggcgacaacc tttccattta tgagcaactc gctgacgatg cccggtggaa ccttcctggg
     6361 tctgaccagc accggggaac ccatcctgct caacccctgg aatacggccc tggaaaatcc
     6421 ccatgccttt gtgggaggag tgacgggagg cggcaaatcg tatctgggga aactctggct
     6481 cgaacgctcc ttgctcatga gtggcgcgag tggcgagcgg tacgcggtta ttgatcctga
     6541 tggcgaatat ctcccactcg cggcggccct gggcggaagc gtcgtgcgga tcgcccctgg
     6601 ctctcaccat catctcaatc cgttcgatct catccctcct ggctgtgatc tgccgacgta
     6661 tctggcgggg gtgcaacgca gtgatcggct ggccgagaag atccaggact tgcagagtct
     6721 cttagacctc atgctggctg acgcggggac caccctgtct gcgcgcgaaa aagcgctgct
     6781 ggatcgtgcc ctctatgaag tctatcgccg cgccagcatc agtgctgacc cccagacgca
     6841 tttccatcag ccaccactct tgcgggatct ggcggaggtg ttgagaagcg gcgcctgcgg
     6901 acctgacgaa tgggatctgg gtctgcggct ctcgcgttac acggagggca gcctggccgg
     6961 gctcttttcc agccagacca atgtcaatct ggatacgcac ctgctcatct gggacacccg
     7021 cgacatgcgg ggtgatatgc gtcccatcgg cattttcctg attgcggacg cgatctggac
     7081 acaggccatc tatcagagtc atatcaggcg ctgcctttcc atcgacgagg ccgcatcgct
     7141 gatcgagcat cccgaaggcg ggcgctttct cgccaatctc tcgcgcagag cgaggaaacg
     7201 ctacctgcgc ctggtcatca tgacccagag tccagagtcc tttgtcgaag atccttctgg
     7261 cagcgtggtg gcgtccaatg ccgcgatcaa aatcctgaaa aaacaggacc ggaccagcat
     7321 tcgcgccgtc gcccagcgct ttggcctgac cagtggagaa gagcaacgcc tgctgctctt
     7381 cggggtacaa gaggcgttac tgttcgccgg tgatcggcgt gtgcttctgt cggttcaggc
     7441 cagtccacag gagcatcgtc tgatcaccac caatcccatg gaacgtgctc agcaggcggc
     7501 tcctgccgtt cctgattcag ggggaaccag gcgtgcaacg tccccaacag ggcactccat
     7561 ccaagaagca gaaggcgagg aatgctaatg tcatcactct cccccctgtt gcaggttcgt
     7621 cctgcacgcg caagcggggc acgcgatctt gtggccctcg aaacagcgct ggccgggctg
     7681 gcactggatg aacacacgcc cattgccctg gaactggcgg ccacgcccac ctcgcgccag
     7741 tttctgctgc gcgcgcagca gccgattgcc ctgcggcacc tcgcacagca gattcaagcg
     7801 cgctacccgc aagccgtgat tgaaccggag cctggagatc cgctcgtctg ccaaccaggc
     7861 gaagcgtgtt ccgtggtcga attgcggcct ggggccgctt cctatctgcc gctgcgctcc
     7921 tggaaagcgc gcgaaatcat cactgaagga acggacccgc tcctgggcgt tctggcgtct
     7981 ttcgggtcac tgcccaccgg cacgcgggca gtcgcgcaac tcgcactcgt gcccgcctcc
     8041 cctacgtggt cgtctcccaa ccgccgctat gcggttgaac cacccctgcg ccacgagcag
     8101 cagaggaggc agggaaccgt cccggagcgt agcctgaaag acgtggttct gctcgtcccc
     8161 gtggttctgc tgctgctggg gctctacctg tttcacggtc tggtgcccac ctggctcgta
     8221 caggcggcgc tggatctcct gcgtggacac ccaccacatc tgagtaccgc cgaaacggtc
     8281 gcggttgtgc ttggcgcggg agccgggctg atcgtgctcc tgggtggagc ctatctggcg
     8341 attctgctgg tgagtcgctt tgagtcccct cggatctatg atcagcgact ggtggcggag
     8401 aaaacggccc gcccggccta tcgcgtgcgt gtgcgcgtgt gggtcttcgg ccctgcgcct
     8461 cacaccaggg gcgcagccac gtcaggctcc cggattgacc ggctcgttcg cgcgggcgtg
     8521 cgcgcctgga agcgctggga agcgtggatg agccacccgc ctcatctgca cgacatcatc
     8581 catcaactgt cgaaccagtg gcaggcccac cgcgtctttg tgcgggaccg acgcgcacgc
     8641 caccaggaac gtaccgctat cctgcgccag attgtgggag cctatcgcca gtatcacctg
     8701 gcggcgggag gctactttct cccgcgcccg ctctcggcga ggcaggcacg ccgcttgctg
     8761 actcctcccg gacgccgggg gttcaggcgc gttggctggg cggcggacct ggctcgttca
     8821 ccccattatc tgagtgtggc cgatctggca gcgctctggc atctccccca ggccgatgac
     8881 ctggcggatc tggtctatgt ccctcacacg accatgcgca ccctgcttgc cccgccaatc
     8941 ctggccaccg gccagggcta tctgctgggt acctccaccc acgccggcca ccaacttccg
     9001 gtgtttctcc cctttgcctg cttgagccag aatctgctgg ccgccgccag caccgggaaa
     9061 gggaaaagta cgctctttta tcacctgagc cgcgccttcg cgcacgggcg tctctcgcac
     9121 gaggcgggcg tgcccaatgg gatgctgctt attgatcctc acggcgatct tgtccatcag
     9181 gtcgcgggcg cacttccctc agcgctggct gatgatgtgg tggtgatcga tctggccgac
     9241 cgggactatc cactggcatg gaatccgctg gatatggccg gaaccgggaa ggaccgcgat
     9301 aaggtgattg atcatcttat ccaggtcatc gaagcgctct ggccaacaag ctatggaccc
     9361 cgcaccgaga gttttctaga atatagctgc aagacgctcg ccgaagcgaa tgggcgcctg
     9421 ctggccgatg atccgctgac cggaccagat cgccagtata cgctacttga tgtcgttccc
     9481 ctcttgcgac gcgccagctt tcgtcaccgg gttctcgaac aggtcgatga tccgtatctg
     9541 ctcacctggt ggcagcagta ttatgagttg ctggacgcgc ggcaacaggc cgactttacc
     9601 agttcgctga tcaccaagct cgctaaattt tccagcagcc gcatcgtcag ccgcatcctg
     9661 gggcagcccc gttcgaccat cgacctggga gatctcattc gccagaacac catcatcctg
     9721 ttctcctgcg ccgcagggga aatcggggcc gatatggccg cgctctttgg ctccctcttc
     9781 gtgggctttt ttcaggcggc gttacaggag caggccagcc tcgccgtcag cgaccgccac
     9841 cgctttctcg tgctcattga tgaattccag gcgctggccg ggatcaatta tcaatcaatg
     9901 ctggctgaac tgcgcaaata cggcggaagc tttgccctgg ccacccagtc gctcgcctat
     9961 ctcgaccgct tcgagcgtac cctgcgcgcg acagtactgg ccaacacgga gcatctgttc
    10021 gccttcgcga tggccgacga agacgcccgg ttgcttcggc tccccggcat cgatccagag
    10081 gatatcgtgc atcttgccgc atatacctgt tacgcccggt tgtcgctgca gggcgagcgc
    10141 ctgcccctct tttccttgca cctgcaagcc ccagaaccag aggatcgctc gcttcggcag
    10201 acgattgttg atcgctgtcg ggcacgctat ggccgcccgg tgggcgaggt tgagcgtatc
    10261 ctgcgcgatg gcctggtgcg gcaaagcgtc cctggggatc tggggaaggc ccacgggtgg
    10321 gatttagagg gcgtggaaac ggtggctgaa gcggtggaac gcatcaaaaa gaaacgcaaa
    10381 cgcggcagcg gcgccagcaa aaaagccgcc gcatccgatc tccagggaac cgcaggagcc
    10441 gtgccctctc acctcctgta cgaggaagag gaagcatctc ctgatgcagc agaggacact
    10501 gagaaaggag gtgcctgatg cctccgtccg ctcacgcgct ggctctgacc ctggaggaag
    10561 gcgcccttcg cttgcttctg tggcttgacc actatcccct gcaacgtgct caggatctga
    10621 tcctggcggc tgctccctgg gaaaagcgca ccaccgtcta tcggcatctc gccgacctgg
    10681 agcgcgccca tctgattgag gaactctacg cgggaatcac cgccaaaaag cgcctctacc
    10741 atctcagtcc tctggggtgc gctgtctgtg attacctggc agcacaagaa gatcatcctc
    10801 aagcggagaa acaggcgcga tgggaacggt ggacgcgcca ggatgtgggg gcggtggttc
    10861 gagaagaacg aggcaaactg gtgcgcctgc tgccgcgcct gccgctgtgg gttctccttc
    10921 aggacacgat tgaggggctg gttcggaatg cagatcgcgc tctcgctcgc caggggcggc
    10981 acgcccaatt gatccagtgg aactggatgc gcgagtatcg ccatcccttc gtgtcgcgtg
    11041 agcagacgct gcgtgtccag gctgaggccg cgctcacctt ttgtctgcgc tttccccagg
    11101 cacccggggc acctccctcc tcttctccgc tggccaccga agaggtctgg tacaccatga
    11161 tggtgttgca ctgcccgctg gatgagccgc gcctgatgcg ccagcggctg gatcggctac
    11221 tccgttggcg cgagtcggct gaacgcatgg gggtttacag tcacatgcct ccggtcgtga
    11281 tcctggcgac caccgaacgg caggccgagt ggtggcacca gctcgccgcg cagactgccg
    11341 cacgcctgcg agttgatccg ctggttggtg cggtggcggt gctgccaccc gaaccggtct
    11401 cctgctggca cctgccgttt cgacgcctgg acaccagaga aatgtgccat ctccacaatc
    11461 tgctttcgcc cctgccagtc cccgcgctgc ccgaactgct ggcttcccgt ggagacgccc
    11521 gcaggagcaa agcggcgcga gaaacacgcg ccgccaggac agtgcttacc cttccccttc
    11581 cttcgcgtct gcgggggcgt tctttcctgc cagggaaagc gcgtgtcccg gtgcgccggg
    11641 gttccccgcc tcagcagagg acgcctggcg actattgcgc actcagcgtg gccctgaccg
    11701 cgcggcagtg ggaaatcctc cggctctgtt ttgcccatcc cctgctgtca cgggaggatc
    11761 tcactcactt cctcctgctc agccgcacca cgacgacact gctgctgggg gatctggccc
    11821 gcctggggta tctggcgagc acctccacgc tggtcggaga acgctggcag gtttccgaat
    11881 ccgggttacg tctcctggct cgcctggtcg gctgccaggt gcatcgtttc atccgttttc
    11941 ccgtcgaggc cggacatccc ctggtccagc gcggagtccg aggattgatg catcagatcc
    12001 gtcatacggc aggcgtctac gcgttcttcg cgcaggtgtc ggaggcgctg gcccatcagc
    12061 cggatacccg gttgctctgg tgggaaaccg gcgttctcag cgaacggcat ttcgcgttcc
    12121 gcgaacgcat ctatcggttt cgccccgatg cgttcgccag cgtacagatc gggcagcgcc
    12181 ccatgcggtt ctggctggag tgggatcgcg gaaccatgac cgcgaagaat tatcaggtga
    12241 agtttaccac gtatgccatg tatctcgcct cacgagagtg ggcgcgcagt tccccgttgc
    12301 ttcccgccct cctctgtgtc gcgcccgata tcggccagga gagccagctc accaaagccg
    12361 cccgccgctg tgtgctccag gcgccttcgg gatggtgtgt gtactccacc accgcctcgc
    12421 tcctgatgac gcagggagtc ctgggtccca tctggcgcct tgtcactgcg caggagccga
    12481 ccgaaccgcc ggcggcgctg gagcgtcaac ggctcttcac ggctgaggca ccggaccggg
    12541 ggagcgaacc gctatgaccc tggcgcgacc aacagtgtcc caaaaacaga ccactgttgg
    12601 ccacaaagag gcacactgtt ggtcacaaag aggcacacgt tcccgctcca ttttgggcac
    12661 gtttctttta cacttgaaga agaacaacac cagcaggggc gcaaagaacc ccacgcagcg
    12721 aaaggagcag catgaggaaa cggagtgacg atgggcagcc cgtgcccgga ccagccctgg
    12781 ccggtcatgt catccaacgc tacgaggctc aacatgcctg ggtgatcgat cacctcctgc
    12841 ttcaggggag tgtcaccgag tatcaatgcc tgggcctctt gctggaacgg gccaatacct
    12901 gtgttcccta cgcggcgttc ctgcggcgag ggatcatgac ctcgacgggc aaccgcaaac
    12961 aggaccgggg acggctggcc gccctcatca gtcgcttgcg gtcccggctc tgggggtgcg
    13021 ggttcgagat tgccaatgtc tggaatgtcg gctatctcct gctcgcagat ccgcctgcat
    13081 acagcgacag gacgagaggt cgtcctgaag aactgtcgta gactcgaccg gatgactgcg
    13141 actataccat gtgctgcctg taccgggcag acgagcaagg acaacgagca tgcaacaacg
    13201 accgacagtg agctttatcg ggctgggtca gatgggccag cccatggcac gccacttact
    13261 tcaagggcta cagggcaaac gcgatctctt cgtctttgat gcccaggccg agcgcatgac
    13321 cgcgctcacc gccctggggg cacacggggg aacctgcctg gatgacgtgg tgccgcgcgg
    13381 tggagtggtg tttgcgatgc tgcctgatga tcggagcctc ctgcaggtgg cgctcaccga
    13441 tggcggcatt cttacccatc tgggaacggg gggcacgctc atcgtgtgtt ccagcgtctc
    13501 cccggatgtc tccgcccagc tcgcccggct ttcccaccag cagcactgcg cctatctggc
    13561 ggccccggcg ttgggcggcc cggaagccgc tgcgcgcggt gacctgtcct ttgtgctggc
    13621 cgggccgcgt ctggccaaac ggcaggtgat gcccctgttt gcctgtatgg gacaacgcct
    13681 gtatgatttg ggagaacgcg tggaggccgc caatgtcatg ggactcgcct ggcatctgct
    13741 gcgcgccagt ctgatcgagg cactgggcga agccgccgcc ctgatcgacg cctaccatct
    13801 gaaccggagc cgcctggccg acctcctcca ggcgccgtcg ctgtggcagc aaaccgctga
    13861 cgccgtgcgc aggattggcg cgcgggattt ctctgagccg cagttgtcgg tgcaagcggg
    13921 cctcagaatg cttgatctgg cgctccacct cggacaacag cgtgatctcg atctgcccta
    13981 cgccgatgtg gcctacggcc atttactggc agcgctggac gcggggcgtg agcaggaaga
    14041 ctggtcggtg ctttcggact ttgcccgcca gggagccacc gattggttta ccagggagga
    14101 ggatgccgat gcccccctgg cctgaaactc cctggcaagg catgaccgcg cgccgggaac
    14161 agcatcttcc gatgcatcgc ctggcgctgg atgcggagca cttcaaacgg gtcggacagc
    14221 acgtgctcga acaggcccga acctttctct atgacgagcg cccgatctat cagcccctct
    14281 cgcgcgaaga acgggaacgc tggcagcagg cgcccttgcc agcgcaggga cttgacccgg
    14341 cccacctcct gagcgaattg caggaggtca gccagcacgc gacctttggt atcaaccatc
    14401 gccgttccta tgcctacgtg ggcgggggag tcaatgaatg gggcgtgctg gcctccctgt
    14461 tagccgccgc gctcaatccg aactgcatcg gaggcgatca ggccatcacc tacatggaac
    14521 tggcaacgat tcgctggctg gcggagttgt ccgggtttgc cctgccgcct cacggcgggg
    14581 gagtcctcac cacgggcagt tctcaagcga ctctgctggc actggccgcc gcgcgtcaac
    14641 ggctggcccg ccgcctggga cgcgatatgc gcgtacatgg cttgcagggt ctctcgcccc
    14701 tggccatcta tgtctcggat caaacgcatg ccagtgtgca tcaacaggcc aatgtcctgg
    14761 ggctgggccg cgtgtgtgtc attgcttcgg acgacgaggg gcgcatgcgc ctggatgtcc
    14821 tccaggaaac catggagcgc gatgagcggc tgggcatctt gccctgttgt gtggtggcca
    14881 cggtcggcac cacgaacacc ggggcgattg atccgctgga agccctggcg gaggtgtgtg
    14941 agcgccatcg tctctggttg catgtcgatg gcgcttatgg cggtctgggc tgggttgatg
    15001 agcgcctgcg cgagcgctat cgaggcatcg agcgtgcgca ctcgctgtcc ctcgatggac
    15061 acaaatggtt gggggtaccc tacgaaagtg gctgtctgct ggtacgtgag cagcaggcac
    15121 tgaaagaagc gtttgcccgt ccaacgccgt cctatctcaa gcaggtggag gatgagcatc
    15181 cccatttcag tgacttgagc gcgcaagtga gccgggcacc gcgcgcgctg gtgctatggg
    15241 cggtgctacg ctccctggga tatgccgggg ttcgcagcct ggtcatgcgc caccgggatc
    15301 tggcaacgct gctggccgcc ggccttgtcc acctgccagg gatgcgcctg ctggcccccg
    15361 ttgaactctc caccgtctgt tttcgctatg agccagcctg ggcgcagggc gacgaagagc
    15421 acatccgaac ctgcaatcag catcttctac gcgacttgga gtccacggcg tttctctcga
    15481 ccaccgaact gcatgggcaa tgggcgctac gcgcctgcgt gctgcacccg cgcaccgact
    15541 gggatgatat ccgcctcttc ctggatgcct tgccccccgc acttgcgcgg caagaggcag
    15601 ggaagccatg accatgcgtc tctttcgggt gatgctcgcc aggcacatcg ggcaggagcg
    15661 gatcaccgag ggggtcatgc tgacgccctc gacggccacc aggctcgcct tgcgcctggg
    15721 gcgggtggga caaggcttcc gccgggaaac gatcccggtg ttccatgcgc cgactccact
    15781 gctcacgcat gcctcgctcc gctcgctggc gaacgggcgg gtcgtgctgg acgccacgcg
    15841 cgagacggac tcacgcggca tcctggtggt gctgaccgcc cacaccccct ggaccatcga
    15901 ggcgcacggg agcggggtgc gagaaatcgg ctacggctcc cgctttgcct cgaaccgagg
    15961 aggccggagg gaacgattcg gggtggcgga acggctggta tggctccagc cgggcgcctg
    16021 gatcgtctgc tccgcgtctc caacccggac catctatcat ctgcggtgtc agccccaggc
    16081 gacaatcccg cagacctgtc gcctcctgct ggaggtgaaa cccgcgactc ccaaacgaaa
    16141 ggatcagcgt catgcacgca tacccctctt ctcatcatcc cttactcggc agtcgaagcg
    16201 tccatcccac ccggaccatt gtcacggtcc ccgccgcgtg cgtcgacggt ggccaggtcg
    16261 ctctcggcga tggccatctc gtgatgatgg ccggcccctg taccgtggaa agcgaacggc
    16321 aactgatgac caccgctgaa tgggtgcgtc gtcagggagc cgtcatcctg cgcggcgggg
    16381 cgttcaagcc ctcaacctct ccctatggct tccgtggtct gggagaagcc ggactcaaat
    16441 tgctggccaa agcgcgtgat gcttttggtc tggcgatcat caccgaagtc atgacgccga
    16501 ccgacgtgga gctggtctgc gcatacgctg atctgctgca aatcggcaca cgcaatatgc
    16561 aaaactacat gctgctcgac gcggtgggca aaacgcacac ccccgtggtg ctcaaacggg
    16621 gcatggcagc caccattgaa gaatggctgc tggcagcgga atacattctc gcgcagggca
    16681 atgaccaggt gattttgtgt gagcgcggca ttcgcacctt tgaacccatg acgcgcaata
    16741 cgatggatct cagcgccatt cccgtcgtca agcagctctc gcatttgccg atcattgctg
    16801 atcccagcca gggaaccggg catcgggagt tggtaacgcc cctttctctg gctgctattg
    16861 ctgccggggc cgatgcgctg ctcatcgaag tgcatcccca tcccgaggaa gcgctcaaag
    16921 atggtgcgca atcgctgacc ctggagcagt ttcaggcgct catgccccag gcccaggcgg
    16981 tggcccacgc ggtcgggcgg tcgcttatgg gaccgcgctg aaccgaccgc gcacgacgcc
    17041 tcctgtccct gccagttccc acgcgccggg aaggcggggc aggcgagcgc gtcccctcca
    17101 ttctagcagg acaacagaaa gaagaaaccc attctatgga tgatgctcat ttgggagaaa
    17161 cgcttctcca cctggcgctg gcgatggatt ccgctcgcca tgcctattgg cgcggcacgc
    17221 ccgcccacga cctgctccaa cgcgacacgc aggtgtacca gcacctggcc caggaacgcc
    17281 ccaggggcat ggaactcatg atcgatctgt atcactgcga tccggctcgc ctccgtgatc
    17341 ctcagcatct gcatgccttc gtcaccgcgc tcacggcctt gctctcccgg tatcagcctg
    17401 gggaatccct gatcacccgc gtgggaccgc acgcgcagag gcgtggaagc accctggtac
    17461 aatggcatga cac
//
LOCUS       DASZYN010000332        10470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_269774, whole genome shotgun sequence.
ACCESSION   DASZYN010000332 DASZYN010000000
VERSION     DASZYN010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 10470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10470
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269774"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2056)
                     /locus_tag="VHE33_08625"
     CDS             complement(<1..2056)
                     /locus_tag="VHE33_08625"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HVT97559.1"
                     /translation="MPRLRRSSPLLAIAALALLGLALPYRAQADPSPSATAPHLNVSL
                     LVPPAELYPGLSFTAGLDFKLEDGWHVYWINAGDSGEPPAITWKLPAGVTASPMQFPP
                     PKRLPLGPLMDFGYENEVVFPIPIRVAADYHPAHPAATLAAHVTWLVCREVCIPGKAD
                     IAVSRIAYATAPPAPSSDLGAQGVIAGFQARLPRPLPSSDSATFSSSAKNLILSVTGI
                     KANSAQFFPLDETVIANAAPQPATAASNGIQLTLTKDENLQTPPQKLNGLLELPDGTA
                     YEIHATPGAAAAAATVPPNGSSGILGAISLALIGGILLNLMPCVFPVLFIKGLALVQS
                     SQHERHKLRAHGWVYTLGILVSFWVVVAVLIALRAAGRQLGWGFQFQSPTFLALMAML
                     LFFLGLALAGQFEIGLQLTSAGGGLAQKSGYAGSFFTGVLAMVVATPCTAPFMGAAIG
                     YALAHSAWVSFAVFSALALGLALPYLALAYFPGWARLLPKPGAWMEVLKQAVSIPIVA
                     TVIWLVWVFTQIAGTNALIGLLGAFLLLAVAGWFLGRWPGKAFASMISALFVVVAIGT
                     SAWTARSLGEPSPAQPGSLADMIARGELTKVANSQWRPFTPALLAQSRAQGKPVFVDF
                     TASWCLSCQVNERLILRRADVEQKLHDSGAVLLRADWTNQDPDITQTLAALGRSGVPT
                     YAL"
     gene            2320..3165
                     /locus_tag="VHE33_08630"
     CDS             2320..3165
                     /locus_tag="VHE33_08630"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HVT97560.1"
                     /translation="MPSAVREISIAHSPDSDDAFMFYGLATNKVRVAGHKFTHVLCDI
                     ETLNQRAIREAFFDVTAISFHVYPYIQENYALMPCGGSVGEGYGPMIVAPRAYDLDEI
                     RRVKIAVPGTLTTAYLALKLFAPEIDTAVVPFDQIIPEVMAGNYQAGLIIHEGQLTYG
                     RTGLSKILDLGVWWREQTGLPLPLGGNAVRRSLGDTTMLTVTQAVHDSIQYGLDHREQ
                     ALEYAMQFGRDLDTSLADKFVGMYVNDRTLDYGRDGREAIRKLLAMGHERGVIPHPVQ
                     VDFVE"
     gene            complement(3275..3574)
                     /locus_tag="VHE33_08635"
     CDS             complement(3275..3574)
                     /locus_tag="VHE33_08635"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HVT97561.1"
                     /translation="MRPPVIFTPVAREELIDAQDWYENKVPGLGRRFRAAVDDLIQRI
                     MANPHQFPVIDKNIHRALFRRFPYALMFVIDPNDGVIVIACFHGSRNPMQWRRRA"
     gene            complement(3574..3792)
                     /locus_tag="VHE33_08640"
     CDS             complement(3574..3792)
                     /locus_tag="VHE33_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HVT97562.1"
                     /translation="MDLLSHDELARLTFPERLALISQLWDSLENDDLPLTEPQRAELE
                     RRLQTLDQDQRAGVTWAALKAELEERCP"
     gene            complement(3857..4723)
                     /locus_tag="VHE33_08645"
     CDS             complement(3857..4723)
                     /locus_tag="VHE33_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97563.1"
                     /translation="MSLPLRQSGCIALAILSAVCVSRVPLALAQETEATVNSRYLELD
                     HTASSRLGPADLSLIHDKRSEIAAEAAFFGYDLRAGEWDYDAGTCPVIPGQLILHYRR
                     HFPNGAQSLFTALVPRQTGRVWVVPVLYRNATPFRSATGSQRSIAVFNRVVPPDLAAK
                     AVQPEGDWLSYALCYADIVYGNANILSHPGTEVGLSHAPMPLLRLSEASTNRSIVFTD
                     RNAPGEYLVWNLTMNDKGQMLAATAEQLSDYVAKERNGAQPSEKPMPTGTEPAVKPLP
                     PQKEPTVKPRPQ"
     gene            complement(4791..6341)
                     /locus_tag="VHE33_08650"
     CDS             complement(4791..6341)
                     /locus_tag="VHE33_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97564.1"
                     /translation="MKKLAIASIALATFAAGAISLPALAQGADGQATAQPAGQTGGAN
                     CGQITIKDAAEYNAYANATSQSDPAAKASAIESFLQQYPNSVAKNEMLQGLMAAYQAA
                     GNAPKMLDAAKRLIAADPNNLRAMLAVVYLQNMQANQPANAANKQQMLDEAAATAQKG
                     LSATKDACMSQADYDKVKDLATPTFYSAIGAAAMAKKDTKAEIDAYTNELKSYKDPNQ
                     TTSGPALVDTYNLGNAYLNLDPKDIKNAVWFLTRAAQFAQANFKKPIQDAAEYWYKKY
                     HCAQNDAACNATPPQGFAQVQQLAGVPANIFPPADYNPTQAPPPPSPADLAHQAVVTS
                     AGCAGVTPAPPPSAPATGAGAAATPAAAPETAPAAAPATPAASAPAPSLPAACTTSLK
                     NMALSDKEFILANGQQADQAAIWSVMNGVTAEIPGKVISATPDSIQLAVTQDAQQSNN
                     ADFTVNMKTPLKEKELPATGTTVTLIATFDSYTPKMIILKDGAPKASPKAPVHHAAPA
                     HRSAARPH"
     gene            complement(6658..9240)
                     /locus_tag="VHE33_08655"
     CDS             complement(6658..9240)
                     /locus_tag="VHE33_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97565.1"
                     /translation="MTIRNSLPAIAAPVRPTNLFQVKLTPHRRLSLAACVLLCLATAA
                     SAQVHPQRLIGPPAGSHTVTLTVVDQNSVVVNAAQVLVEEPSQPALRIATDYAGRCTW
                     TPRQSRPYTIRVEKPGFYQSTTSDVDPRDKSLHIVLTREELIQQQVDVVASAPGIDTE
                     QLSDQKALDVPEIANIPYATNIDIRGILPYTPGVIADSSGQIHVAGGETFMTLDTLDG
                     FDIRSPVFGTFDLRLNADAVRSIDTETTRYPVQYGRATGGVVAFTTGTGDNKFRYDAT
                     DFIPSFRNQKGLRFDTFEPRFTFSGPVRRNKLWFFEAIDAQYSQIYIPELPTGADTNR
                     LIRGSNLLKFQTNLAARNTLTAALLFNDYHSPWEGISAITPRESTDKHDVLAWLPWVR
                     DQHSFKNGVMIDGGFAVMRYREGWEPHGSTPFNITPELPNGSNFETLTTRSQRLEAYA
                     DVYLPPRNWNGSHQIRAGIDAEFLRFNQFESFAPINYLREDRTLLRRSTFPTFAPFTR
                     HNLEPAAYIEDRWKPANSVLIEPGLRFDWDQILRRPLFSPRIAFNYTPAGAAGTTKFT
                     GGIGVYYEHTQLDYLTRALAGIRYDNDYAADGTTPTGPALTTTFTADPTSLHEARAIN
                     WSLGLQHRLPAQIYVGGSFMQKRLSNEFVYANQSGPGAIYGNYVLTNQRQDHYYATEI
                     DARKNFSGNYALFGSYTRSSATTNAAIDYVPTLSLLGPQQKGPLFWDVPNRVISWGWL
                     PTWLPAWAPGSASVRKNWDFVYTFDWHTGFPFDSINANNQIVGVAGSHRFPNYVNFSP
                     GLEWRFHFHGKYFGLRGLVENMTNSTDPYIVYNNVDSPQYLTFSQPLGRAFTTRIRLI
                     RSSR"
     gene            9763..>10470
                     /locus_tag="VHE33_08660"
     CDS             9763..>10470
                     /locus_tag="VHE33_08660"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVT97566.1"
                     /translation="MLKLKKIQILGFKSFCDRTEVHLPGQGMAMVVGPNGCGKSNILD
                     AVSWVLGEQSAKSLRGGKMEDVIFAGTRARNPLGMAEVSLTLVDPEAYEGPLLGEPEI
                     VVEDGNPDWDEEKMRAERAAEVEEIIAETQPGVVIGEREVAADDAQGAEPGLAVEGDG
                     TREGEVSAEAAHGEKVPDVVLKIRRRKFNRTPQKGEMIVTRRLFRTGDSEYLLNGKLC
                     RLRDIQDIFMGTGLGPES"
BASE COUNT         2007 a         2471 c         3965 g         2027 t
ORIGIN      
        1 agagggcgta ggtggggacg ccgctgcggc cgagggcggc gagggtctgg gtgatgtcgg
       61 gatcctggtt ggtccagtcg gcgcggagga ggacggcgcc ggagtcatgg agtttttgct
      121 cgacatcggc gcggcggagg atgagccgct cgttgacctg gcaggagagg caccagctgg
      181 cagtgaagtc gacgaagacg ggcttgccct gggcgcggga ctgggcgagg agggctgggg
      241 tgaaggggcg ccactgcgag tttgcgactt ttgtgagctc gcccctcgcg atcatgtcgg
      301 caaggctacc gggttgcgcg gggcttggct caccaaggct gcgggcggtc caggctgatg
      361 ttccgatagc gacgacgaca aacagggcgg agatcatcga ggcgaatgcc ttgccgggcc
      421 agcggccgag gaaccatccc gcgacagcaa gaagcaggaa agctccgagg aggccgatca
      481 gggcattcgt tccagcgatc tgcgtgaaga cccaaaccag ccagatgacg gtggcgacga
      541 tcgggatgga gacagcctgc ttgaggactt ccatccaggc accgggtttc gggagcaggc
      601 gcgcccaacc ggggaagtac gcgagggcga ggtaaggcag agcgagccca agcgcaaggg
      661 cgctgaagac ggcgaagctg acccaggccg agtgggcgag ggcgtagccg atcgcggctc
      721 ccataaacgg cgcggtgcag ggtgtggcga cgaccatggc gaggacgccg gtgaagaagc
      781 tgccggcgta gccgcttttc tgagccaggc cgccgccggc gctggtgagc tggaggccga
      841 tctcgaactg tccggcgagg gcgaggccga ggaagaacag aagcatcgcc atgagggcga
      901 ggaaagtggg ggactggaac tggaatcccc agcccagctg gcggccggcg gcgcggagag
      961 cgatgagcac ggcgacgaca acccagaagg agacgaggat gccgagggtg tagacccagc
     1021 cgtgggcgcg gagcttgtgg cgttcgtgct gcgaggactg gacgagtgcg aggcccttga
     1081 tgaagaggac ggggaagacg cacggcatca ggttgaggag gatgccaccg atcagcgcca
     1141 acgagatggc tccgaggatg ccggaagaac cgttaggagg cacggtggcg gcagctgcag
     1201 cggcgccggg tgtggcgtgg atctcgtatg cggtgccgtc gggaagttcg agcaggccgt
     1261 tgagtttttg cggaggagtc tgaaggtttt cgtctttggt gagggtgagc tggatgccgt
     1321 tcgatgctgc cgtcgcaggt tgcggcgcgg cattggcgat gacggtttca tcgaggggga
     1381 agaactgcgc ggagttcgct tttatgccgg tgacgctgag gatgagattt ttggcggatg
     1441 aggagaacgt cgcggagtcg gaagacggaa gaggcctggg aagccgagcc tggaatccgg
     1501 cgatcacgcc ctgagcaccc agatcactgg agggcgccgg tggagcggtg gcgtaggcaa
     1561 tgcgcgagac agcgatgtcg gccttgccgg ggatgcagac ttcacggcag acgagccagg
     1621 tgacatgagc ggccagggtc gctgccgggt gggcgggatg atagtccgcg gcgacgcgta
     1681 tggggatagg gaagacgact tcattctcgt agccgaagtc catgagcggc ccgaggggga
     1741 gtcgtttcgg cggcgggaac tgcatggggc tagcggtgac gccggcggga agtttccatg
     1801 tgatggcggg gggctcgccg gagtcgccgg cgttgatcca gtagacgtgc cagccgtctt
     1861 cgagtttgaa atcgaggcca gcggtgaagg acaggccagg atagagttcc gcggggggaa
     1921 cgaggagaga gacgttgagg tgcggagcgg tggcggaggg agacgggtct gcctgcgcgc
     1981 gatagggaag cgcgagcccc aggagggcga gggcagcgat agcgagcagc ggagaggagc
     2041 ggcgaagtcg aggcatcgta cggaatcgat tgtaggctgg gcgcattcgg ggctgccaga
     2101 gagacgcatc gagggagcga ttgggttcat cgaaattaca aatgaggatt agcgaatcgc
     2161 atttactgaa gcccatttaa agaggatcac gggcattgaa tggcgtgaca agttgcgaca
     2221 tatccgcagg tctttatagg agaataggag ggagcgcccc cagaaaggga tccggtcttg
     2281 agcgcaacgc ctacacctgc tgagtcaact gctgctgtct tgccatctgc cgttcgcgaa
     2341 atcagtattg cccacagtcc ggattccgat gacgcgttca tgttctatgg gctggcgacc
     2401 aacaaggttc gcgtcgccgg tcacaagttc acgcatgtgc tttgcgatat tgagacgctg
     2461 aatcagcgtg cgattcgcga ggcgttcttc gacgtgacgg cgatctcgtt ccatgtctat
     2521 ccctacattc aggaaaacta cgcgctgatg ccatgcgggg gatcggtggg cgaaggctac
     2581 gggccgatga ttgtggcgcc gcgggcttac gatctggatg agattcgcag ggtgaagatc
     2641 gcggtgccgg gaacgctgac gacggcgtat ctggcgctga agttgttcgc gccggagatc
     2701 gatacggcgg ttgttccctt cgatcagatc attcccgaag tgatggcggg gaattatcag
     2761 gcggggctga tcattcatga gggacagctg acgtatggac gaacagggct gtcgaagatt
     2821 ctggatctgg gcgtatggtg gcgcgagcag acaggattgc cgctgccgct ggggggcaat
     2881 gcggttcggc gatcgctggg cgacacgacg atgctgacgg tgacgcaggc agtgcacgac
     2941 agcatccagt acgggctcga tcatcgcgag caggcgctgg agtacgcgat gcagttcggg
     3001 cgcgatctgg acacgagcct ggccgataag tttgtaggga tgtacgtcaa cgatcgcacg
     3061 ctggattatg gccgggatgg acgtgaggcg atccggaaac tgctggcgat ggggcacgag
     3121 cgtggagtga ttcctcatcc ggtacaggtg gatttcgtgg agtagggaaa ttgaaagcag
     3181 ggaacagcca atagccagta gggaatagga cgtggttgag gctcggcgga tgccgggcct
     3241 tttctattcg ctatgaaatg cgtacaaaca tcggtcaggc gcggcggcgc cactgcatcg
     3301 ggttacggct gccatgaaag caagcaatga caatcacgcc gtcgttggga tcgatgacga
     3361 acatgagagc gtaagggaaa cgccggaaca gggcgcggtg aatgttctta tcgatgaccg
     3421 gaaactgatg gggattggcc atgatgcgct ggatcagatc atccacagca gcacggaaac
     3481 gtcgacccaa tcctggtacc ttgttctcgt accagtcctg ggcgtcaatc aactcttcgc
     3541 gcgcgactgg ggtgaagatg actggtggac gcactagggg caacgttctt cgagctcggc
     3601 cttcaatgct gcccaggtaa caccggcgcg ctggtcctga tcgagggttt gaaggcgacg
     3661 ctccagttct gctcgctgcg gttctgtcag gggcagatcg tcgttctcga ggctgtccca
     3721 aagctgcgag atcagtgcca gccgctccgg aaaggtcaac cgcgccaact cgtcatgaga
     3781 gagcagatcc atacgtctca gcgtacgaaa aaagaagggc cggctcaagc gattcgtgga
     3841 atccgtgata gcagactcac tgggggcggg gtttgacggt cggctctttc tggggtggaa
     3901 ggggtttcac cgcgggttcg gtgccggtgg gcatgggttt ctcggaaggc tgggcgccgt
     3961 tgcgctcttt ggcgacatag tctgagagct gttccgcggt ggcggctagc atctgtcctt
     4021 tgtcattcat ggtgaggttc cagacaaggt attcgccggg tgcgttgcgg tcggtgaaga
     4081 cgatgctgcg atttgtgctg gcttcggaga gccgcaggag aggcatcggt gcgtggctga
     4141 ggccgacttc ggtgcctggg tgagagagga tgttcgcgtt gccgtagacg atgtcggcgt
     4201 aacagagggc gtaggagagc cagtcgcctt cgggctgcac ggctttggca gcgagatcgg
     4261 gaggaacgac gcgattgaag acagcgatgc tgcgctggct tccggtggcg gagcggaatg
     4321 gagtggcgtt gcgatagaga acaggaacaa cccagacgcg tccggtctgg cgggggacga
     4381 gagcggtgaa gagggattgg gcgccgtttg gaaaatggcg gcgatagtgc aggatgagct
     4441 ggccaggtat gaccggacag gtccctgcgt cgtagtccca ttctccggcg cggaggtcat
     4501 agccgaagaa ggctgcttct gcggcgatct cgctgcgttt gtcgtggatc agactgagat
     4561 cggcggggcc aagccgggag gatgctgtgt ggtccagctc gagatagcga gaattgacgg
     4621 ttgcttcggt ctcctgcgcc agggcgagag ggacccggga gacgcaaaca gcgctcagga
     4681 tggcaagagc aatgcagcca gactggcgca acgggaggga catcaggtgt ccaatctaac
     4741 aaaacgggca gccgctgggg ctgcccgtcg cagagggttg atttccaggt ttagtgggga
     4801 cgcgccgcgg agcggtgggc tggagcggcg tgatgcaccg gggccttggg agaggccttg
     4861 ggtgcaccgt ccttgaggat gatcattttg ggggtatagg aatcaaaggt ggcgatgagg
     4921 gtcaccgttg ttcccgttgc cggcagttcc ttttctttca gcggagtctt catgttgacg
     4981 gtgaagtccg cattgttgga ctgctgggcg tcctgagtga cggcgagctg aatgctgtcc
     5041 ggcgttgcgg agatgacctt gccgggaatc tcagcagtga cgccgttcat gaccgaccag
     5101 atggcggcct gatctgcctg ctgaccgttg gcgaggatga actccttatc ggagagcgcc
     5161 atgtttttca ggctggtggt gcaggcggcg ggcagcgacg gagctggagc cgaagcagca
     5221 ggggttgcgg gggcggccgc aggagcggtt tcaggcgccg cggcgggagt agcagccgcg
     5281 ccggccccgg tggcgggagc cgatggcgga ggtgcaggcg tgacgcccgc gcagccggcg
     5341 gaggtcacga cggcctggtg ggccagatcg gcaggcgacg gcgggggcgg agcctgagtt
     5401 ggattgtagt cagcaggcgg aaagatattg gcgggaacgc cggccagttg ctgcacctgg
     5461 gcgaaaccct ggggcggtgt ggcgttgcag gcggcgtcgt tctgcgcgca gtggtatttc
     5521 ttgtaccaat actcggctgc gtcctggatg ggctttttga agttcgcctg ggcgaactgg
     5581 gcggcgcggg taaggaacca gacggcgttc ttgatgtcct tgggatcgag gttcagatag
     5641 gcgttgccga ggttgtaggt atcgacgaga gccggtcctg atgtggtctg gttgggatcc
     5701 ttataggact tgagctcgtt cgtgtaggcg tcgatttcgg ctttggtgtc tttcttcgcc
     5761 atggcggcgg ccccgatagc gctatagaaa gtgggcgtgg ccaggtcctt cactttgtcg
     5821 tagtccgcct ggctcatgca ggcatctttg gtggcggaga ggcctttctg ggcggttgca
     5881 gccgcttcgt caagcatctg ctgcttgttc gccgcgttgg cgggctgatt ggcctgcatg
     5941 ttctgcaggt acacgacggc gagcatggcg cgcaggttgt tgggatcggc ggcgatgaga
     6001 cgcttggcgg cgtccagcat tttgggcgcg ttgccggcag cctggtaagc ggccatcaga
     6061 ccctgcagca tctcgttttt ggcgacgctg ttggggtact gctggaggaa ggattcgatc
     6121 gcactggcct tagcggcggg atcggactgg ctggtagcgt tggcgtaggc gttgtattcg
     6181 gccgcatcct taatggtgat ctggccgcag ttggcgcctc cggtttgtcc agcgggctgg
     6241 gcagtggcct ggccgtcagc gccctgggca agagcaggca gggagatcgc accggcagca
     6301 aaggtggcca gcgcaatcga ggcaatcgca agcttcttca ttcaacgctc ctgggacaat
     6361 ttcatgtgat tcaaatctga gaactcaggc gacctgtgcc taaccagatc agacgcaacc
     6421 attctgcatt gggcgagccg ctgcggcgat gctggattat aaaaaccaat tttgtgtact
     6481 gacaagttga cgcaaacggc actgtctcac accgctgatg ggatgctgtt tggtcaggat
     6541 gatgttgacc gccaaatgca aagagcaaga ttacacgagc tttgaggaaa aagcggggaa
     6601 tttttggggc aggtccagat tgaggaaagt ggttatggcc ggaaacagga tgcggtttca
     6661 ccgggacgag cgaatgagac ggatgcgggt ggtgaaggcg cggcccaagg gctgggagaa
     6721 agtcaaatac tggggggagt cgacgttgtt gtagacgatg taggggtcgg tgctgttggt
     6781 catgttctcg acaagcccgc ggagaccgaa atatttgccg tggaagtgga agcgccattc
     6841 gaggcctggg ctgaaattga cgtaattggg aaagcgatgt gagccggcga cgccgacgat
     6901 ttgattgttc gcgttgatcg aatcaaaggg aaagccagtg tgccaatcga aggtgtagac
     6961 gaagtcccag tttttgcgga ccgaggccga cccgggcgcc caggcgggga gccaggtggg
     7021 aagccaaccc caggagatga ctcggtttgg gacatcccag aagagcgggc ctttctgctg
     7081 cggaccgagg agagagaggg tggggacgta gtcgatcgca gcgttggtgg tggcggatga
     7141 gcgtgtgtac gaaccgaaga gagcgtagtt gccggagaag tttttccggg cgtcgatctc
     7201 tgttgcgtag tagtggtcct ggcgttgatt ggtgaggacg tagttgccgt agatggcgcc
     7261 ggggccgctt tggtttgcat aaacaaattc gttggagagg cgcttttgca tgaagctgcc
     7321 accgacgtag atctgggcgg gcaagcggtg ctggaggccg aggctccagt tgatggcacg
     7381 ggcttcgtga agagatgttg ggtcggctgt gaaggtggtg gtgagcgcgg gaccggttgg
     7441 ggtggttccg tcggcggcgt agtcgttgtc gtagcgaatg ccggcgaggg cgcgcgtgag
     7501 gtagtcgagc tgggtgtgct cgtaatagac gccgatgcca cctgtgaact tggttgtgcc
     7561 cgcggctccg gcgggcgtgt agttgaacgc gatgcgcggc gagaagaggg gacggcggag
     7621 gatctggtcc caatcgaagc ggaggccagg ctcgatgaga acgctgtttg caggtttcca
     7681 gcggtcctcg atgtaagcgg cgggctcgag gttgtggcgc gtgaagggag cgaatgtggg
     7741 gaaagtgctg cgccgcaaaa gagtgcgatc ttcgcggagg tagttgatgg gcgcgaagga
     7801 ttcaaactga ttgaagcgga gaaattcggc gtcgatgccg gcgcggatct ggtgactgcc
     7861 gttccagttg cgcggcggca gatagacatc ggcgtaagct tcgaggcgct gggagcgggt
     7921 ggtgagggtt tcgaaattgc tgccgttggg caattccggg gtgatgttaa acggggtaga
     7981 gccgtggggc tcccagccct cgcggtagcg catgacagcg aaaccgccgt cgatcatgac
     8041 gccgtttttg aagctgtgct ggtcgcgcac ccagggcagc caggcgagga catcgtgttt
     8101 gtcggtgctc tcgcggggcg tgatggcgga gatgccctcc cagggcgagt ggtagtcgtt
     8161 gaagagcagg gccgcggtga gggtgttccg cgcagcaaga ttcgtctgga acttgagcag
     8221 attgcttccc cggatgaggc ggttggtatc agcgccggtg ggtagttcgg gaatgtagat
     8281 ctggctgtac tgggcatcga tggcttcgaa gaaccagagt ttgttgcgcc ggacgggacc
     8341 ggaaaatgtg aagcgaggct cgaaggtatc gaagcggagg cctttttggt tgcggaacga
     8401 cggaatgaag tcggttgcgt cgtatcggaa tttgttgtcg ccggtaccgg tggtgaaggc
     8461 aacgacgcct ccggtggcgc ggccgtactg cacggggtaa cgggtggtct cggtgtcgat
     8521 ggagcgcacg gcatcggcat tgaggcgaag atcgaaggtg ccgaagacgg gagagcggat
     8581 atcgaagccg tcgagggtat cgagcgtcat gaaggtttca ccgccggcga cgtgaatctg
     8641 gccggaggag tcggcgatga cgcccggagt gtaggggaga atgccgcgga tatcgatatt
     8701 ggttgcgtaa ggaatgttcg cgatttcggg aacatcgaga gctttctgat cggaaagctg
     8761 ttcggtgtcg atgccggggg cggaggcgac tacgtcgacc tgttgctgga tgagttcctc
     8821 gcgggtgagg acgatgtgca gcgatttgtc gcggggatcg acgtcggaag tcgtgctttg
     8881 gtagaagccg ggcttttcga cccgaatcgt ataaggccgt gattgacgcg gggtccaggt
     8941 gcagcgaccc gcatagtcag tggcgatgcg gagggccggc tgggagggtt cctcgacgag
     9001 gacctgtgcg gcattgacga cgacgctgtt ctgatcaacg acggtgaggg tgacggtatg
     9061 ggaaccggca ggcggcccga tgagacgctg cggatgcacc tgggccgatg cggctgtggc
     9121 gaggcacagg agaacgcatg ccgcaagaga gaggcgccgg tggggagtga gcttcacctg
     9181 gaacaagttt gttggacgca caggagcggc gatcgcaggg aggctattcc ggattgtcat
     9241 gcaggcttca tgcagatcgg atcggctggt tgtttcttcg gttcggttag gatgcgtttc
     9301 ctttatacgg tggcgatcac gcttcgatgg cgacgatggg ttggaagcgg cgcgacggat
     9361 ggtgggaaac tcttcgccgg cgagggggcg ggcctgggag ggtgctcata actctatcgt
     9421 taacatgggg gattggatgc gggttgctga ggtgcgtttg ggaccggggg agcgaggtgg
     9481 gcgctggcgc ggaaccagcg cggatacagg ttttagggga aatatgcttg cccaaaggat
     9541 caaggagagc taccgttaaa gcatctggca atctccgcga cgcctatcgg tgtcctgtcc
     9601 ccgaggaaga tggcaacgcg gcctaatagt tgggtttggt cccccttatc cacaagatgt
     9661 tggggtggct ctgttgacca tgcttcctgc gggcgctaca gtcgttcgtg aaggaccggt
     9721 acggcctttt cttttcatgg ttcaggagtg attcagaccc ccatgctcaa gctgaaaaag
     9781 attcagatcc tgggctttaa gtccttttgt gaccgcaccg aggttcacct gccgggccag
     9841 ggaatggcga tggtggtggg gccgaacggg tgtggcaagt cgaacatcct ggacgcggtg
     9901 agctgggttc tgggcgagca aagcgccaag agtctgcgcg gcggcaagat ggaagacgtc
     9961 atctttgccg ggacccgggc gcggaatccg ctgggcatgg cagaggtgtc gctgacgctg
    10021 gtggatccgg aggcgtacga ggggccgctg ctcggtgagc cggagattgt ggttgaggac
    10081 ggcaatccgg actgggacga agagaagatg cgtgccgagc gggcggccga ggtcgaggag
    10141 atcattgccg agacgcagcc gggcgttgtg attggggaga gggaagtcgc tgccgatgat
    10201 gctcagggcg ctgagccagg tttggccgtc gagggcgacg gcacgcggga gggtgaggtt
    10261 tcggcagagg ctgcgcatgg cgagaaggtt cccgatgtgg tgctgaaaat tcgccggcgg
    10321 aagttcaacc ggacgccgca aaagggcgaa atgatcgtga cgcggcggct gttccggacg
    10381 ggcgacagcg agtatctgct gaatgggaag ctgtgccggc tgcgggatat tcaggacatc
    10441 tttatgggca ccggcctggg gccggagtcg
//
LOCUS       DASZYN010000333         4838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_332716, whole genome shotgun sequence.
ACCESSION   DASZYN010000333 DASZYN010000000
VERSION     DASZYN010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332716"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="VHE33_08665"
     CDS             complement(<1..1106)
                     /locus_tag="VHE33_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HVT97567.1"
                     /translation="MTEIVTEAPVDATAREAALDIRHSVIVQAPAGSGKTDLLTRRYL
                     RLLAVVDEPEEILAITFTRAATAEMRARILRDLEAAAGRRSPGPDDLERIALARAAVA
                     HAERRGWNIFEHPQRLAIETIDSLCLRIAQDRPFLARLGGHFQPIDEADPLYALAARR
                     TLEHLGEPDGALGEALAQLLDLRDNRLSDCASLIAEMLKVRDQWVSAFPLSRKTAMSE
                     DDWEQLRLRLEEPFHREHRRVLAEAHRLLTSQPAIERQLLELAQYAAGNGNEQAAWLD
                     GVSALTATMPTVHWHAVCDFVLTTDKKWRKSVNVRQGFPASGRDAKERKRSMEELLLW
                     LQHTPNLLDALCAIRRLPPTNYSNEQWTTLRNVFT"
     gene            complement(1099..3831)
                     /locus_tag="VHE33_08670"
     CDS             complement(1099..3831)
                     /locus_tag="VHE33_08670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HVT97568.1"
                     /translation="MPLAPIIADALRRGATVVAASARAARALHLRFAEEQRAQDHTVW
                     ATPAILDWDSWLRGLWRDHAFSSPDAPLLLTSLQERVLWKTAQRDDAALVISSDSMAA
                     LAMEAWSLLSEYQAHPARRSAWDQPDAERFRHWASDFHQLCSRRGWLSASQLESALLQ
                     HLSDPAHIALPAEILLVGFDRLTPAQRGLLGAVRSRGVAVAEFQPDPALASAETRRSW
                     IQATDLRQEIEACARWARDILLQNPGARIGIVVPSIAPVRGNIDRAFRRVLLPASEDI
                     RQQSVHVPWEFSLGHPLSEVPAIRAALLLLRWTAGPLSAEQVSWLMLSGFVADTDTNT
                     LAIANHDARLRRFAVLSTERTLLSYRDSLASSPGLHDLRFRLTDLLRAAEQNRLSSTS
                     RQPSVFADLVPRLLERVAWPGQRPDDSVQFQARQRWQRLLDELALLDFDGTRYSWKEF
                     LDLLEDQAEQTIFAPESHDAPIQILGPFESSGQQFDAVWFLGTDDTAWPQRGRLHPLL
                     PAAVQRQYGMPHAAPGDDWNLAQTVTTRLLHSAPRVVFSFAERDKDAELRPSPLIAGL
                     FPAASEPEPARAAEEPFQFPALESVPDDAGVPAWPPERNAGGADVLKRQAACPFQAFA
                     AKRLRAEALDESEWGISPSEKGKLLHVVMQRLFSSTEPFPIRSRDDLVTAIDTKQLPG
                     ILEAHIDAAMRDQFGALPSDPWQQACLAAEKRRLLIRIGDWLALEAGRQPFAVESCEE
                     RLPDVHIGNLKLNLRADRIDLLPDGSRLLLDYKTGNVSTASWDGERPGDPQLPLYAAY
                     GNVKNVSGVLFARIRSGRIDFEGRVRDAQAQLSADIRSKKALVTHPYSDAMRDEWALA
                     LAHLGDEFLRGEAAVAPRDTKVCDFCDFHSLCRVAESPLVPIDPDENGEEEADD"
     gene            3870..4778
                     /locus_tag="VHE33_08675"
     CDS             3870..4778
                     /locus_tag="VHE33_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HVT97569.1"
                     /translation="MKQLRAHISTACLLAVVVFFAGCHSENRGSAGSGGNQQVGAKKF
                     HVRGIVVSSDAKTGAVTLDTDAIPGYMEAMTMPYTLAQPNVATELHPGDTITATLTVT
                     ANADSLDEIDIIARGKPDYKPAVSFNDLEPGEAVPDFAFLNQSGKIVRLQQWKGKVLL
                     VTFIYTRCPLPDFCVRMSRNFAAIDKQLQKEPQLFAKTHLLSVSFDPKYDTPSVLRSY
                     GEAYTGKYTKETFEHWDFAAPTEKDLAQVLQFFDIGATPEKDKTITHSLSTVVIGPDG
                     KIFKWYPGNEWTPDQVVQDVRKILGS"
BASE COUNT          961 a         1475 c         1591 g          811 t
ORIGIN      
        1 gtgaagacgt tgcgcagcgt agtccattgc tcgttgctgt agttcgttgg cggcaggcgg
       61 cgaatggcgc acagcgcatc gagcaggttt ggggtgtgct gcagccacag cagcagttcc
      121 tccatggacc gcttgcgctc tttcgcatcg cggccactgg caggaaaacc ctggcgaacg
      181 ttgaccgact tacgccactt tttgtctgtg gtgagcacga agtcgcagac cgcgtgccag
      241 tggacggttg gcatggtggc ggtcagcgct gaaacgccgt cgagccatgc agcctgttca
      301 ttgccgttcc cggctgcgta ttgcgcaagt tcgagcagct ggcgctcgat cgcgggctga
      361 ctggtgagca gacggtgagc ctcagccagc acgcggcgat gctcgcgatg aaacggctcc
      421 tccaggcgca ggcgaagctg ttcccagtcg tcttcgctca ttgctgtttt tctggagagc
      481 ggaaaggcgc tgacccactg gtcgcgcacc ttcagcatct cggcgatgag ggaagcgcag
      541 tccgagagcc ggttgtcgcg caggtcaagc aattgcgcga gcgcctcacc gagcgcaccg
      601 tcgggttcgc ccaggtgttc gagcgtgcgg cgcgccgcca gcgcgtacag cggatcggct
      661 tcgtcaatcg gctgaaagtg cccgccgagc cgcgccaaaa atggccgatc ctgggcgatg
      721 cgcaggcaca gggaatcgat ggtctcgatg gcaagccgct gcggatgctc gaaaatattc
      781 catccccggc gctccgcgtg cgccacggca gcccgggcca gggcaatgcg ctcgagatcg
      841 tcgggacccg gggagcgccg gccggctgca gcttccaggt cgcgcaggat acgcgcacgc
      901 atctctgccg tcgcggcgcg cgtgaatgta atcgccagga tttcttccgg ctcatcgaca
      961 acagcaagaa ggcgcaggta gcggcgcgtg agcagatcgg tcttgcccga gccggctggc
     1021 gcctgcacga tcaccgagtg gcgaatgtcg agagcggcct cgcgcgcagt cgcgtccacg
     1081 ggggcttccg tgacgatctc agtcatcggc ctcctcctcg ccattctcat ccggatcgat
     1141 gggcacaagc ggcgactcgg cgacgcggca cagcgaatga aagtcgcaga aatcgcaaac
     1201 cttcgtgtcg cgaggtgcaa ccgccgcctc gccgcggaga aactcatcgc cgaggtgagc
     1261 gagcgccagc gcccactcgt cccgcattgc gtcggaataa ggatgcgtga ccagcgcctt
     1321 cttgctgcgg atatcggcgg agagctgtgc ctgcgcgtcg cgcacgcggc cttcgaaatc
     1381 gattctgcct gagcgaatgc gggcgaagag aacaccgctg acgttcttca cgttgccgta
     1441 ggccgcgtag agcgggagtt gcggatctcc gggacgttct ccatcccagc tcgcggtgga
     1501 aacgttcccg gtcttgtagt cgagcagcaa acggcttcca tcgggcaaaa ggtcgatgcg
     1561 gtcggcgcgc agattcagtt ttaaattgcc gatgtggacg tccggcagcc gctcctcgca
     1621 cgactccacc gcgaacggct ggcggcccgc ctccagcgcc agccagtcgc caatgcggat
     1681 gagcaggcgg cgcttctccg cggcgagaca cgcctgctgc cacggatcgg aaggcagggc
     1741 gccaaactga tcgcgcatgg cagcatcgat atgcgcctcc aggatgccgg gcagctgttt
     1801 tgtatcaatc gctgttacaa gatcgtcgcg actgcggatg gggaacggtt cagtcgaaga
     1861 aaacaggcgt tgcatcacga cgtgcagcag ctttcccttc tccgatgggc tgatgcccca
     1921 ctcactttcg tccagcgctt cggcgcgcaa acgctttgcg gcaaaggcct ggaaggggca
     1981 ggcggcctgc cgtttgagca catcggcgcc tcccgcgttt cgctccggcg gccacgctgg
     2041 aacgccggca tcgtctggca cggattcgag cgcgggaaac tggaatggct cctccgcagc
     2101 acgcgccggc tcgggttccg aagccgccgg gaacagtccg gcgatcagcg gagagggccg
     2161 cagttcagca tctttatccc gttcggcaaa gctgaacaca acgcggggtg cggaatggag
     2221 caggcgcgta gtcaccgttt gtgccagatt ccagtcgtcg ccgggcgctg cgtgcggcat
     2281 tccatactgc cgctggactg cggcgggaag caacggatgc agccgccccc gttgcggcca
     2341 ggcggtgtcg tcggttccca gaaaccaaac agcgtcgaac tgctgaccgg aggattcgaa
     2401 cgggccgagg atctggatcg gcgcatcgtg ggactcggga gcgaagatcg tctgttccgc
     2461 ctggtcctcg agcagatcga ggaattcttt ccaggagtag cgcgttccat cgaaatccag
     2521 caaagcaagc tcatcgagaa ggcgctgcca gcgctgacgc gcctggaact gtacggagtc
     2581 gtcgggccgc tggccgggcc acgccacacg ctcgaggaga cgaggcacga gatccgcgaa
     2641 aaccgagggc tgtcgcgagg ttgacgaaag ccggttctgt tccgccgctc gcagcaaatc
     2701 ggtcagcctg aaacgcagat cgtgcaggcc gggcgaagat gcgagcgagt cacgataact
     2761 cagaagggtg cgctcggtcg aaagcacagc aaagcggcgc agccgcgcat cgtgattcgc
     2821 tatggcgagg gtatttgtat ccgtgtcggc gacaaatcct gaaagcatga gccaggaaac
     2881 ctgctccgcg gacaaggggc ctgcggtcca gcgcagaagc agcagcgctg cgcggattgc
     2941 cggaacttcg ctcagaggat gtccaagaga gaattcccac ggaacgtgga cggactgctg
     3001 ccgaatgtct tcactggcag gcaacaaaac gcgccggaag gcgcgatcga tgttgccgcg
     3061 cacgggcgca atcgagggaa cgacgatacc gatgcgggcg ccgggattct gcagcaaaat
     3121 gtcgcgggcc catcgcgcgc aggcttcaat ctcctggcga agatcggtag cctgaatcca
     3181 ggaacgccgc gtttcggccg atgcaagcgc cggatccggc tggaactccg caaccgccac
     3241 gccacgcgag cgaacggcgc cgagcaggcc gcgctgcgcg ggcgtcagtc gatcgaaccc
     3301 caccagcaaa atctccgcgg gaagcgcgat gtgcgcagga tcggagaggt gctgcagcag
     3361 cgccgattcc agctggctgg cgctgagcca gccccgccgg gaacaaagct gatgaaaatc
     3421 cgacgcccaa tggcgaaagc gttcggcatc gggctggtcc cacgcggaac gtcgcgcggg
     3481 atgcgcctgg tactctgaca gcagcgacca ggcctccatc gccagagcgg ccatggagtc
     3541 ggatgaaatc acgagcgccg catcgtcgcg ctgtgcggtc ttccaaagca cgcgctcctg
     3601 cagcgacgtc aaaagcaacg gcgcgtcggg actggaaaac gcatgatcgc gccagagtcc
     3661 gcgcagccag gaatcccagt cgagaatcgc cggcgtcgcc cacacagtgt ggtcctgggc
     3721 gcgctgctcc tcggcaaagc gaagatgcaa cgcgcgtgcg gcgcgtgcgc tggcggcgac
     3781 gacagtagcg ccgcgccgga gcgcgtcggc aatgatgggg gccagcggca tggaggggga
     3841 atcgttgaag atcaagtgta aactggcggg tgaagcaact tcgtgcgcat atttccaccg
     3901 cctgccttct ggcggtggtt gtgttttttg ccggctgtca cagcgaaaac agaggttcgg
     3961 cgggctcggg cggcaatcag caggtgggtg cgaagaagtt tcacgtccgc ggcattgtcg
     4021 tctcgagtga cgcgaaaacc ggggccgtca cgctcgatac cgacgccatc ccaggctaca
     4081 tggaagccat gaccatgccg tacaccctgg cacagccgaa cgtcgccacc gaactgcatc
     4141 ccggggacac gatcacggcc acgctgacgg tgacggccaa tgctgactca ctcgacgaga
     4201 tcgacatcat tgcgcggggc aagccggatt acaagccggc ggtcagcttc aacgatctcg
     4261 aacccggtga ggccgtcccc gactttgcct ttctcaatca aagtggcaag atcgtccgtc
     4321 tgcagcagtg gaagggcaag gtgctgctcg tgaccttcat ctacacgcgc tgcccgctgc
     4381 cggatttctg cgtgcgcatg agccgtaact ttgctgccat cgacaagcag ctgcaaaagg
     4441 agcctcaact cttcgcgaag acgcacttgc tgagcgtgag cttcgatccg aaatacgaca
     4501 cgccatccgt tttgcgcagc tacggcgagg cctacacggg caagtacacg aaggaaacgt
     4561 tcgagcactg ggacttcgca gcgccgacag agaaggatct ggcccaggtg ctgcagttct
     4621 tcgacatcgg ggcgacaccg gagaaggaca agacgatcac ccactcgctg tcgacggtgg
     4681 tgatcgggcc ggatgggaaa atcttcaagt ggtatccggg caacgaatgg acgccggatc
     4741 aggtggtaca ggacgtgcgg aaaattctgg gctcgtaagt tctatatgat tttggtctga
     4801 ctacgcaggg gagacaaacg atgagcaacg cgctgacg
//
LOCUS       DASZYN010000334         4031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_15451, whole genome shotgun sequence.
ACCESSION   DASZYN010000334 DASZYN010000000
VERSION     DASZYN010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15451"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            951..2651
                     /locus_tag="VHE33_08680"
     CDS             951..2651
                     /locus_tag="VHE33_08680"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HVT97570.1"
                     /translation="MFLQRVFLSTSLLAVAIPALVAFSAPAPAPAHVHFADITQKAGI
                     HFTYNNGAFGKKYLPETMGPGVAFIDYDNDGWQDIFLVNGDAWPGHPGPTTTAHLYHS
                     NHDGTFTDVTRKAGLAVALYGLGVAVGDYDNDGWDDLFVTTYGQNHLFRNNHNGTFTD
                     VTKKSGLTTPPEFSASAAWVDYDRDGHLDLLVTNYVDWSPAKDRYCSMDGKTKSYCTP
                     ETYPGASLRLWHNNGNGAFTDVTRKAGLYDTSSKSLGIAVLDANQDGWPDFAISNDTQ
                     PNKLYINNRNGTFTEQGVPAGIAYSDDGLARAGMGIDAADYDRSGYPGILLTNFSNQM
                     LSLYQNQHNGLFVDTAPSSEVGRSSLLTLGFGCFFFDYDLDGWPDFYVADGHLDDSIE
                     RIQQRIRFAEPPHLYHNLDGKGFADVAAQMGQSFAAPRVARGAAYGDINNDGAPDIVI
                     TTNNGPAALFLNQGATNHALRIRLIGTKSNRDGIGAVVSVKSATTFQKQMLRSGSSYL
                     SASELVLTFGLGSSAKADSIEVRWPSGAVDHLTNTNADQIVTIREGSGLLHAHPLARR
                     "
     gene            complement(3184..3924)
                     /locus_tag="VHE33_08685"
     CDS             complement(3184..3924)
                     /locus_tag="VHE33_08685"
                     /inference="COORDINATES: protein motif:HMM:NF024221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HVT97571.1"
                     /translation="MRTSNPALNDKVFRGLPGALGETMTLEGTVNKTGILLLCAAATA
                     AWTWHLAMNPATMPMDFLLMLVGLVGGFIFAMVTVFKKTWAPVTAPAYALLEGLALGG
                     LSATLEVRYPGIAIQSVCLTFGTLFVLLMAYRSGMIRVTQKFRMGVVAATGAIFLFYL
                     AEMILGFFGIHFASVNGSGALGIGISLVIVCVAALNLVLDFDFIERGVAASAPKYMEW
                     YGAFGLMVTLVWLYLEMLRLLAKIRSRD"
BASE COUNT          906 a         1534 c          892 g          699 t
ORIGIN      
        1 caggttcacc ccacagcaag cagaagactc tcttgccgtc cgctcgtcca gcgattccat
       61 ttgctgaaca agccgaaagt accactccta tgccgtttcg gaaccacctc ccaggaaagc
      121 caattaccca ctcggaaaga gccaggttca ccccacagca agcagaagac tctcttgccg
      181 tccgctcgtc cagcgattcc atttgctgaa caagccgaaa gtaccacccc tatgccgttt
      241 cggaaccacc tcccaggaaa gccaattacc ccttcggaaa gagccaggtt caccccacag
      301 caagcagaag actcctcccg gatcgcgtcg tcaaccgctt ctcacccttc cccgctgcct
      361 aaagtactga gctcagaccg ttttggaact cgccgcccaa ggttctcatt gatcgcccgc
      421 tcaggttcac ccgagagcaa gccacacgcg tcttcgcgaa acagaaacgc aaagccaaac
      481 aacaccctgc ctggcgaaca ccggccctcg tttcgggaac agcggtagtt gccttctgat
      541 gaacgaagtt cgccaacctg ccgcccaaaa ccctggacac gccaaggttc accccaaagc
      601 aagtagcgaa cgccctcgcg ggactgaaat caacgcgaag gatgtccttg ccacaccgaa
      661 gtaccaagct ccgatccgtt tcgggaacgg ccgtcgctgc cttttggtaa atgaagttcc
      721 ccaacctgcc tccccccaaa aaacaaaaca cagccaggtt caccccacag caagtagcac
      781 acgccctccc ggtcgcttcg tcaaccgctt ctttccctcc gtccctgccc gaagtaccgc
      841 ctcagacccg ttttggaaat tgcaaccgca gatgtcctgc tcgactccgc ggcactgaag
      901 gccccctcgg ctgcaccctc caccccgtac cctgtaccct gtatttctga atgtttctcc
      961 agcgcgtatt cctcagcacc agcctgctcg ccgtcgccat tcccgctctc gtcgctttct
     1021 cggcccccgc tcctgcgcct gctcacgttc acttcgccga catcacccag aaggccggca
     1081 tccacttcac ctacaacaac ggcgccttcg gaaagaaata tcttccagag actatgggtc
     1141 ccggtgtcgc cttcatcgac tacgacaacg acggctggca ggacatcttc ctcgtcaacg
     1201 gcgacgcctg gcctggacat cccggcccca ccaccaccgc ccacctctac cacagcaatc
     1261 acgacggcac cttcaccgac gtcacccgca aagccgggct cgccgttgcc ctctacggcc
     1321 tgggtgtcgc cgtcggcgac tacgacaacg acggctggga cgacttattt gtaacaacgt
     1381 atggtcagaa tcatttattt cgtaacaatc acaatggcac attcaccgac gtcacgaaga
     1441 aatccggcct gaccactccg cccgagttca gcgcctccgc cgcctgggtc gactacgatc
     1501 gcgacggcca cctcgatctc ctcgtcacca actatgtcga ctggtcgccg gccaaagatc
     1561 gctactgcag catggacgga aaaaccaagt cctactgcac accggaaacc taccccggcg
     1621 cctcacttcg cctttggcac aacaacggca acggcgcctt taccgacgtc accaggaaag
     1681 ccggcctcta cgacacgtcc tccaagagcc tcggcatcgc cgttctcgac gccaatcagg
     1741 acggctggcc ggacttcgcc atctccaacg acacgcagcc caacaagctc tacatcaaca
     1801 accgcaacgg cactttcacc gagcagggcg tgcccgcagg tatcgcgtac tccgacgacg
     1861 gcctcgcccg cgccggcatg ggcatcgacg ccgccgatta cgaccgctcc ggttatcccg
     1921 gcatcctgct taccaacttc tccaaccaga tgctctccct ctaccagaac cagcacaacg
     1981 gcctcttcgt cgacaccgcg ccctcttctg aagtcggccg ctccagcctt ctcactctgg
     2041 gcttcggctg cttcttcttc gattacgatc tcgatggctg gcccgatttc tatgtcgccg
     2101 acggacatct cgacgactcc atcgagcgca tccagcagcg catccgtttc gccgagccgc
     2161 cccatctcta tcacaatctc gacggcaagg gttttgccga cgtcgcagcc cagatgggcc
     2221 agtccttcgc tgcgccccgc gtcgctcgcg gagcagccta tggcgacatc aacaacgacg
     2281 gcgcgcccga catcgtcatc accaccaata atggaccagc cgcgctcttc ctcaaccagg
     2341 gagccaccaa ccacgccctg cggatcaggc tcatcggaac gaaatccaac cgcgacggca
     2401 tcggagcggt ggtctctgtc aaatccgcga ccacattcca aaagcaaatg ttgcgcagcg
     2461 gatccagcta tctttccgcg agcgaactcg tcctcacctt cggcctcggc tcttctgcaa
     2521 aggccgactc catcgaagtc cgctggccct ctggcgccgt cgaccacttg accaatacaa
     2581 acgccgatca gatcgtcacg atccgtgagg ggtctggcct tctgcacgcc cacccactcg
     2641 ccaggcgatg atccaaccag aacctgatcc aactgaagtg atgattcaac cgatgcgatg
     2701 gcccaaccac gaagcggtga tccaaccaaa gcgccgatcc cgcccaggcg ccgatcccac
     2761 caaaacgacg acgccaccag gcaaccgcca ctgagtgctg atggcgccag gttcacccca
     2821 cagcaagcag aagactccca cgggaacgac tcgtcaaacc atttttcctc ttgctgcaac
     2881 cgacagtacc gtctcctgtt ccgtttttaa aaaggcgacc ccatcccaac atagactcga
     2941 ttggcgaggc gccaggttca ccccacagca agcagaagac tctcttcccg accgacctgc
     3001 aactcaaacg aacctcttct tcagacaaaa gtaccgtcga tcgaaccgtt tcgggaaacc
     3061 agcctcagca cccgcgcaac tcctctccgc ctccaacgga tatctcacgg agctctgtcc
     3121 ccaacacgcc gaaaacaaaa gcgcgggcca aagcccgcgc tccctgcttc cagcgctcga
     3181 gtctcagtcc cggcttctta tcttcgccag caaccgcagc atctccagat aaagccagac
     3241 cagcgtcacc atcagcccga acgcgccgta ccactccata tatttcggcg cactcgcggc
     3301 caccccgcgc tcgatgaagt caaaatcgag caccagattc aacgccgcca cacacacaat
     3361 gaccaggctg attccgatcc cgagcgcccc cgacccgttc accgacgcaa agtgaatgcc
     3421 gaagaacccc aaaatcatct ccgccaaata gaacaggaaa atcgcgcccg tcgccgccac
     3481 cacacccatg cggaacttct gcgtcactcg aatcatcccc gaccggtacg ccatcagcag
     3541 cacgaacagc gtcccaaacg tcaggcacac cgactgaatc gcgatccccg gataccgcac
     3601 ctccagcgtc gctgacagcc cacccagcgc cagcccttcc agcagcgcat acgccggagc
     3661 cgtcaccgga gcccaggtct tcttaaagac cgtcaccatc gcaaagatga acccgcccac
     3721 caggcccacc agcatcagca ggaaatccat tggcatcgtc gccggattca tcgccagatg
     3781 ccatgtccag gccgccgtcg cggccgcgca caacagaagg atgccggtct tgttgaccgt
     3841 gccctccagc gtcatcgtct cgcccagcgc cccaggcagc ccccggaaca ccttgtcgtt
     3901 cagcgccggg ttcgaagttc tcatcaggtc agccacttcc tcttgtcctc cgcggaccac
     3961 ccccgctcca accgcgcaga agccccgctg acctcatgat atgcgctcag cttcgtctct
     4021 ccccgcgcca c
//
LOCUS       DASZYN010000335         1860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_527689, whole genome shotgun sequence.
ACCESSION   DASZYN010000335 DASZYN010000000
VERSION     DASZYN010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527689"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VHE33_08690"
     CDS             complement(<1..275)
                     /locus_tag="VHE33_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HVT97572.1"
                     /translation="MDLLRFNGAVDRDPRIDAWLNEHRGELGAMAREWFGVMRQCGDE
                     FRELLHDGCPTACFGDVPFGYVNVFRAHVNVGFFQGAGLRDPARLLEG"
     gene            376..1662
                     /locus_tag="VHE33_08695"
     CDS             376..1662
                     /locus_tag="VHE33_08695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HVT97573.1"
                     /translation="MKFRQVTGPIVGDRNIAVQPAQPAIGWILLALVVLACSARPAVA
                     FRAHSPRIVHAWILIDAKTGQVLEAWHPQRRAHPASLTKLMTLYLTFRSLENGRINLD
                     TPLHVSRHAAAQQPTRLWLQPGTTITVRDAILGIITRSANDAAVVLGEYLGGSESRFA
                     AIMNREARHLGMTRTRFYNASGLPDARQWTTARDMATLAIALIHTFPQYYHFFGVHSF
                     SFQGRIIYGHDHLLDECDGVDGMKTGYVRASGFNIVTSAIREHRRLVGVVLGGASAVA
                     RDRQMVALMNQGFGMSPVPRSLLAGATNPDQTPASPTAAKLLTTSAEDAPTADTRDWT
                     IETGGNFPTQHAVRRILHSARRSAPSFLRHGRPLVVLLHRRHYRARFSDLTHEEAYQA
                     CSALERRKFTCRVLTHSPSPTQDLASAAASSSSQSD"
BASE COUNT          335 a          704 c          491 g          330 t
ORIGIN      
        1 ccttcgagca gccgcgccgg atcccgcagc cctgcgccct gaaagaatcc cacgttgacg
       61 tgcgctctga acacgttcac gtagccgaaa ggcacatctc cgaaacacgc cgtagggcag
      121 ccatcatgca gaagttcccg gaactcgtcg ccgcactggc gcatcactcc aaaccactcg
      181 cgtgccatcg ctcccaactc cccgcgatgc tcgttcaacc acgcatcgat cctgggatcg
      241 cggtcaacag cgccattgaa tcgcagcagg tccattctca tcccgtatca aaacactaaa
      301 tgggtgtccc cctccgcacc ccgttcaacc cacagcgccc ggtggtaagg tgcagcctgg
      361 agggttgagc agcggatgaa gtttcggcag gtgactgggc ccatcgtcgg cgatcggaac
      421 attgcagtgc aacccgccca gcccgccatc ggctggattc ttctcgccct tgtggtgctc
      481 gcctgcagcg cgcgtcctgc tgtggccttt cgcgcccatt ccccgcgcat cgttcacgcg
      541 tggatcctca tcgacgctaa aacggggcag gttctcgagg cctggcaccc gcagcgccgt
      601 gcgcatccag cctcgctcac caagctcatg acgctctacc tgaccttccg cagcctcgag
      661 aacggcagga tcaacctcga caccccactt cacgtctccc ggcacgccgc cgcgcagcag
      721 cccacccgtc tctggctgca gcccggcacc acgatcaccg tgcgcgacgc catccttggc
      781 atcatcaccc gctccgccaa cgatgctgcc gttgtcctgg gcgaatacct gggcggcagt
      841 gaaagccgct tcgccgccat catgaaccgc gaggcgcgcc acctcgggat gacccgcacc
      901 cgcttctaca atgcctccgg cctgcccgat gctcgccagt ggaccacggc ccgcgatatg
      961 gccaccctcg ccatcgccct catccacaca tttccgcagt attaccactt cttcggcgtc
     1021 cactccttca gtttccaggg ccgcatcatc tatggccacg atcacctgct cgatgagtgc
     1081 gacggcgtcg acggtatgaa gaccggctat gtccgagctt ccggtttcaa catcgtgacc
     1141 tccgccattc gcgagcatcg ccgccttgtg ggcgtcgtgc tcggcggcgc cagcgccgtc
     1201 gcccgcgatc gacagatggt cgcgctcatg aaccagggct tcggcatgtc cccagttccc
     1261 cgctctctgc tggctggagc cacgaaccca gaccagacgc cggcctcccc aaccgccgcg
     1321 aaactcctca ccacctccgc ggaagatgcc ccaaccgcgg atacgcgcga ctggacgatt
     1381 gaaacgggcg gcaacttccc gacccaacat gcggtccgac gcattcttca cagcgcccgt
     1441 cgcagcgcgc cctcgttcct ccgccacggc cgcccgctgg tggtgctcct tcaccgacgt
     1501 cactatcggg cccgcttctc cgacctcacg cacgaagagg cgtaccaggc gtgctctgcc
     1561 ctcgagcgca gaaagttcac ctgccgggtg ctgacgcatt ccccatcacc gactcaggac
     1621 ctcgcgtctg ccgccgcatc ctcttcgagc caaagcgact aaagatcggc cggaatttcg
     1681 gagcctgggc aacagggcgc ggatttgcaa tccccaggga caccccttga ggcaggattc
     1741 agatttgaaa attgactacg atccggtacg gaccgaacgg ctgatcaccc gcgtcgagcg
     1801 caccggcttt ttcgccgagc gaaccagctt cggggtgggc gcctcttctt cgactccgga
//
LOCUS       DASZYN010000336         5844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_848485, whole genome shotgun sequence.
ACCESSION   DASZYN010000336 DASZYN010000000
VERSION     DASZYN010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848485"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..804
                     /locus_tag="VHE33_08700"
     CDS             211..804
                     /locus_tag="VHE33_08700"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVT97574.1"
                     /translation="MSEPSEAIPRQDDTSETTQLLRAWAGGDPEALERLTPHVYRTLR
                     RIAGAHLRNERPGNTLQATALVHEAYLELIDITNVDWQHRAHFFAVSAQIMRHILLDR
                     ARRRVAAKRGGHAERVDLDELPDLSGNRAEELIALEDALNALAAFDQRKARVVELRFF
                     GGLSVEESATVLGISPETVMRDWKFSRSWLQAELSRH"
     gene            945..1409
                     /locus_tag="VHE33_08705"
     CDS             945..1409
                     /locus_tag="VHE33_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97575.1"
                     /translation="MTPMQSIRRTFALAALATTVLLSQAVHAQVNTYASRVTVPFAFN
                     CGGQTFAPGTYTIKMENRSFLVVSNLAQSGFAMVQGDTETRGNAQGYLMFRKYGDRYF
                     LGEYHPANSLASANVVQTKSERRAAREFASNQADQGRIRLALLDDGVGSPRR"
     gene            complement(1485..2252)
                     /locus_tag="VHE33_08710"
     CDS             complement(1485..2252)
                     /locus_tag="VHE33_08710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HVT97576.1"
                     /translation="MKGKLLLGPLFLLCTPTLLTIAEPAPSLQKVTFPSGGDTATAYL
                     ALPTGSGRHPAIVVIQEWWGVNDWVREQAQQFANEGYVALAVDLYRGRIATDAEMAHE
                     LMRGLPQDRGVRDLTSAVAWLKKRPDVDPARIGAIGWCMGGGYAAQLAIAAPSLKAVS
                     INYGSLPTDKSALEHIHAAVLGNFGGQDRGITPDDVHAFQSAMESLGKHPDLKIYPDA
                     GHAFENPNNTGGYRAADAEDAQTRSHRFLAEHLHPRQ"
     gene            2409..3449
                     /locus_tag="VHE33_08715"
     CDS             2409..3449
                     /locus_tag="VHE33_08715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HVT97577.1"
                     /translation="MQRTTLFSGPDGAQGGDARAPAVVHAEGRRGRSWTWSRWRHRVR
                     RRWRETRCLLKILLKELARTQVLDVAAGLAFWSMLSMVPLLITVASLMSVLRIPSLLP
                     QLLSVLAMLVPSDALSTVEKMIGSLLQPHRGVLSFGILSYIWSTTGGFTALITALNVA
                     YDVATPRSWVRDRLQALVLALTSGGLLTVSLLALIAGPHFGHFLAEVLPMPRSFERTW
                     PVIRIATVFVTFVVGLELVYFLGPNRKQQFKSTLPGAIVAIALWFVGSAGLAFYLDHL
                     AHYSRLYGGMGAIIGLMFWIYLTALAILIGAELNAELAKRRDSLFRAHLKEAERERMA
                     AEKGLLRKRPAA"
     gene            complement(4222..5592)
                     /gene="ftsZ"
                     /locus_tag="VHE33_08720"
     CDS             complement(4222..5592)
                     /gene="ftsZ"
                     /locus_tag="VHE33_08720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HVT97578.1"
                     /translation="MNRKPIAQPEEIRIQYHDEVPRGARIKVIGVGGGGGNAVNRMIA
                     ARMEGVEFIAANTDRQALQLSSAPVKLQLGVKLTSGLGAGANPDVGRQAALEDSGKIL
                     EALEGADMVFVTAGLGGGTGTGAAPVIASLASEMGALTVAVITRPFAFEGKRRMAQAE
                     RGMQELLDSVDTMIVIPNEKLLAVAKDAGFFESFRIADDVLRQGVQGISDIITIPGII
                     NRDFADVKTTMAGMGYAVMGTAVRSGAQRAIEAAQAAMASPLLEEGAIDGARGILINI
                     TGSSSLKLSEVNQASTLIQTAAHEDANIIFGAVLDESMGDEVKITVIATGFRQGSAER
                     RDHLLTAALREVAAPRIEKRPPTPRFASEEHDDESAARETPLLIPVTARDFVSAFRVH
                     EAEPATDTPSAQTSAGAEEETELVGVAVAASPAQSDDVEMPHSSFVEEPGENLDIPAF
                     MRKGAF"
     gene            complement(5582..>5844)
                     /locus_tag="VHE33_08725"
     CDS             complement(5582..>5844)
                     /locus_tag="VHE33_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97579.1"
                     /translation="SAPAFFYWDTTSQPAELTFDTKERQGATLVVPSSAPKSIRALAP
                     APLYQGTASAVPFHDLVRQGLQPLRPYVALKTRPKGVNADEP"
BASE COUNT          951 a         1592 c         2114 g         1187 t
ORIGIN      
        1 cgaatgtcgg gatcggcacc ggcgagagcc tctccgccct tttcgcgggc ggagtgatac
       61 agcgcctcaa tctgttgcca gcgatcggaa gtcaagcatt accccgggtg tgaatgaatg
      121 gtattattgc gccgaacagc gtccaattga cacgatattg cgccacttcg gcaaagttat
      181 gccggaagtt atcacgggga ggcgcgggct atgtcggaac catctgaagc gataccgcga
      241 caggacgaca cttcggaaac gacgcagctt ttgcgagcct gggcgggggg agaccccgag
      301 gctctggagc ggctgacgcc tcacgtgtat cggacgctgc gacggatcgc gggagcgcat
      361 ctgcggaacg agcggcccgg caacacgctg caggcgacgg cgctggtgca cgaggcgtat
      421 ctggaactga tcgacatcac gaacgtcgac tggcagcatc gcgcgcactt tttcgctgta
      481 tcggcgcaga ttatgcgcca tattctgctg gaccgggcgc ggcgccgcgt ggcggccaaa
      541 cgcggcggac atgcggaacg cgtggatctg gacgaactgc cggacctgag cggcaaccgt
      601 gccgaagaac tcatcgcact ggaagatgcg ctgaacgcgc tggcggcgtt tgaccaacgc
      661 aaggcccgcg tcgtggagct gcgattcttt ggcggactca gcgtggagga gtcggcgacg
      721 gtgctgggaa tctcgcccga gacggtgatg cgcgactgga aattctcgcg gtcgtggctg
      781 caggccgagc tcagccgcca ttaagcggct tcttaaaaaa aatcttcatt gggtgacagt
      841 tttctccttc gccttgcgcc tacagggcaa agccgttcag agacaggctc ccagcgcact
      901 cttctctgat ccgctgcaga gttcatcccg aaaaggagaa agccatgacc ccgatgcaat
      961 cgatccgccg cactttcgcc ctcgccgctc tcgccaccac cgttctcctg tcgcaggccg
     1021 tgcatgcaca ggtcaacacc tacgcttcgc gcgtcaccgt gccgttcgca ttcaattgcg
     1081 gaggccagac ctttgcccct ggtacctaca cgatcaaaat ggagaaccgc agctttctgg
     1141 tcgtgagcaa tctggcacaa tccggcttcg cgatggtcca gggcgatacg gaaaccaggg
     1201 gcaatgctca ggggtatctg atgttccgca aatacggcga ccggtatttt ctgggcgagt
     1261 atcacccggc caacagcctg gcgtcggcga atgttgtgca gaccaagtcc gagcgccgcg
     1321 ccgcccgcga attcgcctcg aaccaggcgg atcaggggcg gattcggctg gccctgctcg
     1381 acgacggtgt cggctcgccg cggcggtaag ctgctgctgg ccggtatcgg aaaaggggag
     1441 ccccctcacg gaggctcccc tttcgctttt tggggacagc ttcttcattg ccgggggtgg
     1501 agatgttcgg cgaggaagcg gtgggagcgc gtctgggcgt cttcggcatc ggcggcacgg
     1561 tagccaccgg tgttgttggg gttctcaaag gcgtgaccgg cgtcgggata gattttcagg
     1621 tcgggatgtt tgccgaggga ttccatggcg gattgaaacg cgtggacgtc atccggtgtg
     1681 atgcctcgat cctggccgcc gaagttgccg agcacggctg catggatgtg ctcaagagca
     1741 gacttgtccg tcggaagcga gccgtagttg atggagacgg ctttgaggga gggcgcggcg
     1801 atggcgagct gggcggcgta gcctccgccc atgcaccagc caatggcgcc gatgcgggcg
     1861 gggtcgacgt cggggcgttt cttcagccag gcgacggccg aagtgaggtc gcggacgccg
     1921 cgatcctgag gcaggccgcg catgagttcg tgggccattt cagcatcggt ggcgatcctg
     1981 ccgcgataga gatcgacggc aagggcgacg tagccttcgt tcgcgaattg ctgtgcctgc
     2041 tccctgaccc agtcattgac gccccaccac tcctgaatca cgacgatggc gggatggcgg
     2101 ccggagccgg tgggcagagc cagatacgca gttgcggtgt caccgccgct gggaaaagtg
     2161 accttctgca ggctgggggc gggttcggcg atggtgagaa gagttggagt gcagagcaga
     2221 aagaggggcc cgagaaggag cttgcctttc atcgtgttcc tcctggtgaa atcactcata
     2281 tatcgcagaa gagggcgacg gcaggcaacc gggagacggc gaagaagaga aagtctgcgg
     2341 aatctgcgcg catccatgca gggctattct gccatcgcaa caaggtagcc ggtgtgccgg
     2401 agcgccttgt gcagcgaacc actttgtttt cgggccccga tggagcgcag ggcggagacg
     2461 cccgggctcc ggcggtggtg cacgcggaag gtcggagagg gcgcagctgg acttggagcc
     2521 gctggcgtca tcgggtgcgg cggcgctggc gggaaacccg atgtctgctg aagattctgc
     2581 tgaaggaact ggcgcgcacg caggtgctgg atgtggccgc ggggctggcg ttctggtcga
     2641 tgctgtcgat ggtgccgctg ctgatcacgg tggcgtcgct gatgtcggtg ctgcggattc
     2701 cgagcctgct gccgcagttg ctgagcgttc tggccatgct ggtgccgtcg gatgcgctgt
     2761 cgacggtgga gaagatgatc ggcagcctgc tgcagcctca tcgaggcgtg ctgtcgttcg
     2821 gcattttgag ttacatctgg tcgacgacgg ggggatttac ggcgctgatc acggcgctga
     2881 atgtcgccta tgacgtagcc acgccacggt cgtgggtgag ggatcgcctg caggcgctgg
     2941 ttctggcact gaccagcggc gggctgctga cggtgtcgct gctggcgctg atcgcgggac
     3001 cgcacttcgg gcattttttg gcagaggttt tgccgatgcc gcgctcattt gagcggacct
     3061 ggccggtgat tcgaattgca accgtgtttg ttacgtttgt cgtgggcctg gaactggttt
     3121 attttttggg accgaaccgc aagcagcagt tcaaatccac gctgccggga gcgattgtgg
     3181 cgatcgcgct gtggttcgtg gggtcggcag ggctggcgtt ctaccttgac catcttgcgc
     3241 actattcacg gctgtatggc gggatggggg cgatcatcgg gctgatgttc tggatttatc
     3301 tgacggcgct ggcgatcctg attggggcgg aactgaatgc ggagctggcg aagcggcggg
     3361 actcgctgtt tcgggcgcat ctgaaggagg cggagcggga gcggatggcg gcggaaaagg
     3421 gtttgctgcg gaagcgcccg gcggcttgag gagccggaac tcgcgttggt tgtctgtgag
     3481 ggccggaaga ttccggaaat ggcacagacc tgggtacgga tgggttgagc ggggtgggaa
     3541 tgctgttgaa tcctggggcg ggaaggcgtt tgctgcttgc tgtggggtga acctggcgct
     3601 tcccatgctg gggtgctcgg tggtggatgt ttcccgaaat ggtccggcag cgtgacttcg
     3661 gattggctcg gggcgagaac tgggttgact ccgtgtgttc tgaaggcgtc cgctgcttgc
     3721 tgtggggtga acctggcgct ttccatgccg gggtgctcgg tggtggatgt ttcccgaaat
     3781 ggtccggcag cgtgacttcg gattggccgg gggcgtgaac tgggttgact ccgtgtgttc
     3841 tgaaggcgtc cgctgcttgc tgtggggttg aacctggcgc tttcccccaa agggggcgcc
     3901 cggtgttaac tcttcgcctg gtggcgcttg ttcgcttcct gctgtggggt gaacctggcg
     3961 cttccccaaa ggggccgccc ggtgttaact ccttcgcctg gtggcgcttg ttcgcttcct
     4021 gctgtggggt gaacctggcg cttcccccaa agggtgtgcg cccggtttaa ctccttcgcc
     4081 tggtggcttg ttcgcttctt gctgcgttgt cgtcttcctg gtggtgtgcg ttacgttttg
     4141 ggagtcgtca ttctcgcgac cgcgcagggc tcccgcggtg gtggaccacg ccgataaccg
     4201 ctgagcggcg tgggaccgag atcagaacgc ccctttgcgc ataaaggccg ggatgtcgag
     4261 gttttcgcct ggttcttcga cgaaggatga gtgaggcatt tcgacatcgt cggactgagc
     4321 gggcgaagca gccaccgcta caccgaccag ctcggtttcc tcttcggcac ctgcggaagt
     4381 ctgggcggat ggggtgtcgg tggccggttc agcttcgtga acgcggaagg cggagacgaa
     4441 atcgcgcgcg gtcacgggga tgaggagggg agtttcgcgg gccgcggact cgtcatcgtg
     4501 ctcctcgctg gcaaagcgag gcgtgggggg gcgtttttcg atgcggggcg cggcgacctc
     4561 acgcagagca gcggtgagca gatggtcccg gcgttcggca gaaccctgcc gaaagccggt
     4621 cgcgatgacg gtgatcttga cctcgtcgcc catcgactca tcgaggacgg cgccgaagat
     4681 gatgttggcg tcttcgtgcg cggcggtctg gatgagggtc gaggcctggt tgacttcgct
     4741 gagtttgagc gagctggagc cggtgatgtt gatgaggatg ccgcgagctc cgtcgatggc
     4801 gccttcttcg agcagtgggg aggccatggc ggcctgggcg gcctcgatcg cgcgctgcgc
     4861 gccgctgcgt acggcggtgc ccatgacggc gtagcccatg ccggccatgg tggtcttcac
     4921 gtcggcgaag tcgcggttga tgatgccggg gatggtgatg atgtcggaga tgccctggac
     4981 gccctggcgg aggacgtcgt cggcgatgcg gaaggactcg aagaagccgg catctttggc
     5041 cacagcaagg agcttctcgt tggggatgac gatcatggtg tcgaccgagt cgagcagttc
     5101 ctgcatgccg cgctcggcct gggccatgcg gcgcttgcct tcaaaggcga aggggcgggt
     5161 gatgacggcg acggtgagtg cgcccatctc gctggcgagc gaggcaatga cgggagcggc
     5221 gccggtaccg gtgccgccgc cgagtccggc ggtgacgaag accatgtcgg cgccttcgag
     5281 ggcttcgaga attttgccgg agtcctcgag ggcggcctgc cggccgacat cagggttggc
     5341 gccggcgccg aggccgctgg tgagcttgac gccgagctgc agtttcaccg gggcgctgga
     5401 gagctggagt gcctggcggt cggtgttggc ggcgatgaac tcgacgcctt ccatgcgggc
     5461 agcgatcatg cggttgacgg cgttgccgcc gcctccacct accccgatga ctttgatccg
     5521 tgcgccccgg gggacttcgt cgtggtactg aatgcggatt tcttcgggct gagcgatggg
     5581 tttacggttc atcggcattg actcctttgg ggcgggtttt gagtgcaacg tacggcctca
     5641 ggggctgaag cccctgtctt accaggtcgt ggaacggcac ggctgaagcc gtgccctgat
     5701 acaaaggcgc aggggctaaa gcccggattg atttcggtgc ggacgacggc acgaccaggg
     5761 tcgcgccctg acgctctttc gtgtcgaagg tgagttcggc aggctgtgag gtcgtgtccc
     5821 aatagaagaa cgcaggggct gaag
//
LOCUS       DASZYN010000337        13437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_848522, whole genome shotgun sequence.
ACCESSION   DASZYN010000337 DASZYN010000000
VERSION     DASZYN010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 13437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13437
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848522"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VHE33_08730"
     CDS             <1..798
                     /locus_tag="VHE33_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HVT97580.1"
                     /translation="SPTKADVEAFLKASWGYDENRVWEVYGIEKTTAPGVSKVTILVA
                     EKQTPQQIANLTFFVTPDGKHLIAQESVLDFGAKPYENNYRLLQQRAEGPSRGATAKQ
                     FELVEFGDFQCPHCKEAQSIGEKLTQDFPQARFVFENFPLVSIHPEAYKAAAWGTCVA
                     QEGGAAAFFKYADSVFAAQNDLAGQGADQALRNAANAAGADPGKIATCADSTAGKNPV
                     DASLRLGHELNVNETPMLFIDGRGVPMMAVPYEQLKKIVEYQFSLDK"
     gene            804..1406
                     /locus_tag="VHE33_08735"
     CDS             804..1406
                     /locus_tag="VHE33_08735"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HVT97581.1"
                     /translation="MAIEPTFDPAKTAVLCMDMQAAIIEIYTAGQPDGHQLVSRAAEV
                     LQRCRERGLTVIHVQVGFRPGLPEVNPRNALFGAILNSPERQKLFEGDAGRVHPALGP
                     VEGDIVVTKHRISAFTGTDLEMILRAKEIETVVMFGIATSGVVLSTLLDAVDADYRAM
                     VLGDCCADRDAEVHRVLVEKFFPQRGTVVSAADWLKSLDR"
     gene            complement(1429..1842)
                     /locus_tag="VHE33_08740"
     CDS             complement(1429..1842)
                     /locus_tag="VHE33_08740"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HVT97582.1"
                     /translation="MSSSFERIRPSVALVGRVVLGAIMLAHGWGKVVPRGSLYNFAHV
                     VARLGLPYWLGYVAAFTEFFGGIALILGLLTPLVALAVAIEMLVAILKIHLRHGLTGA
                     QGYEFPLSLLALSLLLIADGPGYLAVDRVVFRGGR"
     gene            2153..2380
                     /locus_tag="VHE33_08745"
     CDS             2153..2380
                     /locus_tag="VHE33_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97583.1"
                     /translation="MAKRRGNPNWGKPEPIGPVVPTVTSFEQVVKEFKLTPDQYVRST
                     RLREWARRNRNSKYIPEALLEAWGFEIESTL"
     gene            complement(2483..3406)
                     /locus_tag="VHE33_08750"
     CDS             complement(2483..3406)
                     /locus_tag="VHE33_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97584.1"
                     /translation="MRRVLLPVLMTALALAPYALASSDPERAANGSVPPADVTASNPA
                     TADLNAANPGVNASFLPPLPLAPSGKSTVIGGVIRSIDPVLDEMTLGVYGNGKPMKIY
                     FDQRTKFYRDGVKTPLDDMRPNDHASVETLLDGDMVFAASVHMLSGSPEGDCQGQVLA
                     FDPRNGEVTVRNSLSGAPIKLRVEPRTTIARIGQPAFASSVTGTSDLMMGALISVKFR
                     SDNRGNGIADSISILATPGSGFYFAGNVTYLDVHAGMMAITDPRDQRSYTIAFNPTSF
                     PASRNLHIGSRVGATASFNGKQYVASKLDVY"
     gene            3588..4763
                     /locus_tag="VHE33_08755"
     CDS             3588..4763
                     /locus_tag="VHE33_08755"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT97585.1"
                     /translation="MIAKKFSWFLGTAAMLAFCLPHSHGQYLKIKIPRRSELTPVQRL
                     NREGVEEIRKQRYEKAEELFYKAYLYDPSDPFTLNNIGYIAELQGQFERAQRFYKLAA
                     EQGTNASIDISNEKQLDGKKMKAALESLQDLPMRLNRMNFDAMTLLSQNRPTEAMNLL
                     QAARRMDQNNAFTLNNLGVASEASGDYDLALRYYAAAAAARSNEAAVVTTTGAYRGQP
                     VSQMAAGNQQRLEQRMEDLKTDPARAALLASRGVAAVNRNDWETARKDFVEAYRLDPY
                     SAFSLNNAGYVAEHDGDIETAQFFYAKAEQADDAHARVGTATRPGAEGNPLATIADNN
                     SQDMQEKLDLEREIRRRQTGPIELHRRDGTPVKATPPAQPASPDGSSPDTTTPQQPQ"
     gene            4792..5907
                     /locus_tag="VHE33_08760"
     CDS             4792..5907
                     /locus_tag="VHE33_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97586.1"
                     /translation="MSNPSAPTPSPKSMLLLSRLLLSPAFADSDERGRSSLPGSELLN
                     LSREHFEELVALASSNHVIVRGMERYVAAMRSRGDAMREEWAQDALAMERTRIANAIP
                     FLRSICDAFEEENQDVLVIKSLDHWPDLGSDLDLYTPASPETVLELMRRRFNAQLAPR
                     SWGDRLARKWNFLIPGLPEAVEIHVGRLGQTGEQMVLASSVARRARKIQVDGVPFRTT
                     HISDRLMISALQRMYRHFYFRLCDIIDSAALIEGELIDYDDLEHAATAAGIWEGMATY
                     LVIVADYVSLYRGAGPHLPQTVTSNARFGGEKIFYAKEFLRVPILPQSAGLYGSQLGG
                     LLRKGELESSVRLGLLPWLAGAAVVGQKITGSDKGIW"
     gene            complement(5923..7383)
                     /locus_tag="VHE33_08765"
     CDS             complement(5923..7383)
                     /locus_tag="VHE33_08765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT97587.1"
                     /translation="MKICLVATFPPSGRQLNEYAFHIARELQRHPGVELTILADRLTK
                     YDFATDGNGHSLDGQQQEELPGFNVIRCWDFGSLKTPLRLLQTIRRIKPDVVWFNLVF
                     SSFATPENPVAAFAGLSAPALVRSMGFFTHITLHHILEHVDFNAAGVRKSRLLQLGSD
                     FATRALLKADSVTVLLPAYQRTLMAKYSAKNVLLSTHGTFAATPDPPDFSKRGNPDQR
                     ILAIGHWGTYKRLETLMEAFPKVLEKVPNARLVIAGANHHTRAGYWESIRAAQPAHLP
                     IDFLGYVAEEDIPALYSSTSALVMPYDSATGSSGPAHQACEYGVPIVSADIPDLREMV
                     IDEDMEAAFYRTGDANALADELIAILSSPELQRRMAEHNYRAGVRMTMSCVVRNYLRW
                     FELQQCKKAMENGARVHLNGMQPASKDDPFDDPPSPHALQDEFALDTEAPLELSSDGD
                     AALSSADPVRSQSSRGPHEASLLNSYSSRPMFEVEF"
     gene            complement(7395..11642)
                     /locus_tag="VHE33_08770"
     CDS             complement(7395..11642)
                     /locus_tag="VHE33_08770"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT97588.1"
                     /translation="MPIVRPESPRIARVLLFLLLFSGPVFAQTYKVGSDDNTKSQSNK
                     TQPAATQPQSQEKQLGWGSNIQNARLAGAAEAALKRGDHQQAVTYAQRAAQSAPNNPQ
                     LWFLLGYAARLNAQTGLAVDAYNHGLRLEPSSVLGLSGLAQTYAVMGRTKEAQDMLQK
                     VLAVDPKQIDDAVLLGDLQIRSGNYDDAVKTLDQAERFQGNARSELLLAMAYQHQNKL
                     DQSRHYLEMAKARAPNNPEVQRAMAGYYRETGHYDEAIAALKSIKSQRPDIKAELAYT
                     YQLDGNAEEAARLYVQVANAVPKDLNLQLSAAQAEVAVGSIHNADTFLTRAAAIDPEH
                     YRLHALRGEIARIQEKDNDAVREYQAALAHIPADPVEGPLYPIQLHMDLMELYKNTHQ
                     PDQADQQLKTAQAAINALDENGTDRGPFLRLRAMIRMDTGDLTGARSDLNAALAINAT
                     DPNSLQLDGDLLVKMGHTEEAIAVYKKILTIDPKNRFALTSIGYASRAAGRDQDAEKY
                     FERLAQAYPTLYVPWLAMGDMNASRKEYTKAEADYAKAYALAPDNALVVAGGLNADIE
                     GHKLDAAATWFNRATKDMEDEPQVLREQERYLSFKGQYPESLAKGERAIQGLPNDRDV
                     VVYMGYDLLHLNRYDDLLALTKKYDAVLPKEPDIPLLAGYVYKHNNQLEEARQAFTET
                     LQRDPSVVTAYVNRGYVLHDLHQPQAAEADFEAALKRDSKDGEAHLGLAYTSLDLHKP
                     RLALRQVALAESALGESQAIHLVRATAYGQQGALPKAAREYRAAIRYTPNDGSLHLAL
                     ANVLYTERRFRDAVDELNVAQKLAPDNAMIYAWLARSWAELDNPAETMQYVKIAEQRA
                     MLKPASAKSPGESDAAQIFLLTGESLNTVGDHAAAMERFRRALTLPGSDRVGVRLAIA
                     EVMAQKNQTQDARREIALSLMEAESGEAKLPTGAQMIQVGDLFRQMHDYQLSEDYLQR
                     AEAAGASDTAVRVGLANTYLALGDTPRAQGELAAISNSPDEEPDYQYLLAKASVYRQE
                     HRNPQALTAFAQATDAAGEDQTAEQNLLQTGADEGLRLNNHFSVLADAMVSPIFEDTS
                     VYVLDSKLDGPVPVAPTDTSLLPPPRSSLQTEETNAFHLHLNYLPPASGFFQVRNARG
                     TISVPATSSIVNRNTTDYGFNFGVNPTARIGNNVITFNSGIQATVRRDSEDPVDLNQN
                     LFRLFTYVSTSSFFNVVSMDGFVLRETGPFTESNIHSTAFSAAVNFRVGAPWGRTALI
                     TGWGENHQKFDPVSTEDYYTGSYIGLDRQFGTRAQVKGIVEDLRAWRTFQGRSGIAQV
                     LRPAGTVNFRVTPRWSLQGGAAWSSNRSFHVYDAVQSNLSVSYAMPFHREFRDQTGDV
                     VLQYPIRFSAGVQQETFFNFTGGQSQQFRPFVSITLF"
     gene            11782..>13437
                     /locus_tag="VHE33_08775"
     CDS             11782..>13437
                     /locus_tag="VHE33_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97589.1"
                     /translation="MQKIETVAPSSRPIPMSFILLAVFAVHLPLLLMELPLKSYDTNF
                     NIFFASHYVHHWFDPWNAKWYAGFSQTTYPPLPQQWVAVVSWLTGLNLAYMTVQFVGI
                     LLLVVGVYRFAKLWVSPRAASFAALATVFLGSEAFLIYSAGQLSTTCAAPLYLNALPY
                     LFEWIREGKGRSFLKGAVLFTAAAAAHHATLLFGSMFFAIPVVALALIDKRGDEPVPR
                     GAIIGRTIAITIVVGVAVAIVLLPFWLALIHYPVTQTPIPHASRANYILSPQWGVNYF
                     IVPWGALILALPFIFWRGASVPRLRPLLIGFWVAFLCGLGGTTPVGHWLLGRAFEVLT
                     FERFSYWATLLAVPFVGLLASELVDRFRFRAIAGLAVLAAATCALAVSWSTFHPAEGE
                     EFRVDGVANWLNRDGHDNYRYITLGFGNQISRLAILTDAGSLDGEWNSGRMLPELQAH
                     GGGALTSAKFFGKDGLDSLHAILDHADRYGLRWVFVRDPFYDPLLAFGGWRHVDELNA
                     GTIAVWTKDGVPPATPVNAPQMPPAWQGIMWGTLPFGSSLLAIL"
BASE COUNT         2557 a         3606 c         4558 g         2716 t
ORIGIN      
        1 tcgccgacga aggccgacgt cgaggcgttc ctgaaggcgt cgtggggcta cgacgagaat
       61 cgcgtgtggg aggtttacgg catcgagaag acgaccgcgc ctggcgtgag caaggtgacg
      121 atcctggtgg ccgagaagca gaccccgcaa cagatcgcga acctgacgtt ctttgtcacg
      181 ccagatggca aacacctgat cgcacaggag tcggttctgg atttcggggc gaagccgtac
      241 gagaacaatt atcgattgct gcagcagcgc gccgaggggc cgtcgcgggg cgccacggcg
      301 aagcagtttg aactggtgga gtttggggac ttccagtgcc cccattgcaa ggaggcgcaa
      361 tcgattgggg agaagctgac gcaggatttt ccgcaggcgc gcttcgtctt cgagaacttt
      421 cccctcgtga gcattcatcc ggaagcctac aaggcggccg cctggggtac ctgcgtggcc
      481 caggagggag gggcagccgc gttcttcaaa tatgctgact cggtcttcgc ggcccagaat
      541 gacctggccg gtcagggtgc ggatcaggcg ctgcgcaacg ccgcgaacgc ggccggtgcg
      601 gacccaggga agatcgccac atgcgctgac tcgacggcgg ggaagaatcc ggtggatgcg
      661 tcgctgcggc tggggcatga gctgaacgtg aacgagacgc ctatgctgtt cattgacggg
      721 cgcggggtgc cgatgatggc ggtgccgtat gagcagttga agaagatcgt ggagtatcag
      781 ttctcgctgg acaagtaggg ctcatggcaa tcgaaccgac gtttgaccca gcgaagaccg
      841 ccgtcctgtg catggacatg caggcggcca tcatcgaaat ctacacagca gggcagccgg
      901 atggtcacca gctggtttcg cgcgcggcag aagttctgca gcggtgccgg gaacgaggcc
      961 tcaccgtgat tcacgtgcag gttggattcc ggccgggctt gccggaggtg aatccacgta
     1021 acgcgctgtt cggggcgatc ctgaactcgc ctgagcggca gaagctgttc gaaggcgatg
     1081 cgggccgggt tcacccggcg ctcggaccgg tcgagggcga catcgtggtc accaagcatc
     1141 ggatcagcgc tttcaccgga accgatctgg agatgatcct gcgagcgaag gagatcgaga
     1201 cggtggtgat gttcggcatc gccacgagtg gggtggttct ttcgacgttg ctggatgcgg
     1261 tcgatgcgga ttaccgggcg atggtgctgg gggactgctg cgccgatcgg gatgctgagg
     1321 tgcaccgggt gctggtggag aagttctttc cgcagcgcgg caccgtcgtg agtgcagcgg
     1381 attggctgaa gagcctggac agataagagg agagtggcgg ggctgaggct agcggccgcc
     1441 acggaagacg acacgatcca cggcgagata gccggggccg tcggctatga ggagcaggga
     1501 gagggcgagg aggctcaggg ggaactcata tccctgagcg ccagtgagcc cgtgccgcaa
     1561 gtggattttg aggatggcga cgagcatttc aatggcaacc gccagagcga caagcggagt
     1621 caggagaccg agaatgagtg cgatgccgcc gaaaaactcg gtaaaggcag cgacgtagcc
     1681 gagccagtac ggcagtccca ggcgagcaac gacatgcgca aagttgtaca aagagccgcg
     1741 ggggaccact ttgccccatc cgtgcgcgag catgatggct cccaggacga cccggccgac
     1801 cagcgcgacc gacgggcgaa tacgctcaaa actcgacgac aaatcctgct cctccaatca
     1861 cttttcccga ggatacctga agcgggagac gggctcgatt cgagcctgcg gaaaggattg
     1921 gccgtggcgt caacagcaga ttactgctcg cagtgcgact tcacccgctt ttggacatct
     1981 aattgaaaga tgaggcagtt agattcgagg tacgcctctg acgagaggca ttttcctctt
     2041 gattttcata gaaaaaatac ctataataat aaagtttgtg actacccgag agggtctgta
     2101 gggcctccgg attttccgtt cgtcactttc caaagcagag ggtaaatcat acatggcaaa
     2161 gcgtcgtggc aatcccaact ggggcaagcc ggagccaatc ggaccggtag tgcccaccgt
     2221 gacctctttt gagcaggtgg tcaaggagtt taagctcacc ccggatcagt atgtccgttc
     2281 cacccgtttg cgcgagtggg ctcgccgcaa tcgcaactcg aagtacatcc ctgaggcgct
     2341 gctggaagcg tggggcttcg agatcgaatc caccctttga ttcggtccgg taaccgagtt
     2401 gtaccagacg ccgccttcgg gcggcgtcgc tgtttgtggg atgtgcgacc gaccctgagg
     2461 cgcggccgcc gggaggaggg gtttagtaga cgtccagctt actcgccacg tactgcttgc
     2521 cattgaagct ggctgtggcc cccacgcgcg atccgatgtg caggttgcgg ctggcaggga
     2581 acgaggtggg attgaaggcg atggtgtagc tccgctgatc gcgcggatcg gtgatcgcca
     2641 tcattcctgc atgcacgtcg agataggtca cgttgccggc gaagtagaag ccggatcccg
     2701 gggtagcgag gatcgaaatc gagtccgcga tgccattgcc ccggttatcg gagcggaact
     2761 taaccgaaat aagggcgccc atcatcagat cggaggttcc ggtcactgaa gaagcaaagg
     2821 cgggctggcc gatccgggcg atggtggtgc ggggctcgac gcgcagcttg atcggggctc
     2881 cagagaggga attgcgcacg gtaacctctc cattgcgtgg atcgaaggcc aatacctggc
     2941 cctggcaatc gccttccggg gagcctgaaa gcatatgcac actggcggcg aagaccatgt
     3001 cgccgtcgag aagggtttca accgaggcgt gatcgttcgg gcgcatatcg tcgaggggcg
     3061 ttttgacgcc gtcgcggtag aacttggtgc gctggtcgaa gtagatcttc atcggcttgc
     3121 cgttgccata gacgccgagg gtcatttcat cgaggaccgg gtcgatgctg cgaatgaccc
     3181 cgccgatcac ggtgctcttg ccggatgggg caagcggaag cgggggcagg aagctggcat
     3241 tgacgccggg gttggcggca ttgagatcgg ctgtcgccgg attggacgcg gtgacgtcgg
     3301 ccggcggaac ggatccattc gcagcgcgct ccgggtccga gctcgccagc gcatacggcg
     3361 caagcgccag ggcggtcatc aaaacgggaa gaagaacgcg tcgcatgggt ggcaggctcc
     3421 tgaaactttc ctcggcggat tggatggcgg aagtgggatg ccggttggct gggtggtgca
     3481 gtttggcgct gccaattccg gcaaacgctg gggctgattt ggcatctaag cggtagattt
     3541 caactgtccg gccctggttg ggccggttac gcacgagaaa aacaggcatg atcgcaaaga
     3601 aattctcatg gtttctgggc acggcggcga tgctggcatt ctgcctgccg cactcgcatg
     3661 ggcaatatct gaagattaag attccccggc ggagcgaact gacgccggtt cagcggctca
     3721 atcgtgaagg ggttgaggaa atccgcaaac agcgctacga gaaggcggag gagctgttct
     3781 ataaagctta cctgtacgat ccgtcggatc cctttacgct gaacaatatc ggctacatcg
     3841 cagagctgca ggggcagttt gagcgggcgc agcgttttta caagctggcg gcagagcagg
     3901 ggacgaacgc atcgatcgat atcagcaacg agaagcaact ggacggcaag aagatgaagg
     3961 cagcgctgga aagccttcag gatctgccga tgcggctgaa tcggatgaat ttcgatgcga
     4021 tgacgctgtt gtcgcagaat cggcccacgg aggcgatgaa tctgctacag gcggcgcgcc
     4081 gaatggacca aaacaatgcc ttcacgctga acaatcttgg cgttgccagc gaggccagcg
     4141 gtgattacga cctggcgctg cgctactacg cggccgcggc cgcggcccgc tccaacgagg
     4201 cggccgtggt gacgacaacg ggcgcctacc ggggacaacc ggtgagccag atggcagctg
     4261 ggaatcagca gcggctcgaa cagcggatgg aggacctgaa gaccgatccg gcgcgggcag
     4321 ccctactggc gtcgcgcggg gttgcggcgg tgaatcgcaa cgactgggag acggcccgga
     4381 aggattttgt ggaagcttac cggctggatc cttacagcgc gttttcgctt aataacgcgg
     4441 gatacgtagc cgagcacgat ggagacattg agacggcgca gttcttctac gccaaagcgg
     4501 aacaggcgga cgacgctcat gctcgagtgg gaacagcgac acggcctgga gcagaaggaa
     4561 acccgctggc aacgatcgcg gacaataaca gccaggacat gcaggaaaag ctggatctgg
     4621 agcgcgagat tcgccgccgg cagacaggcc ccatcgagct gcatcgtcgc gatggcacgc
     4681 ctgtgaaggc gacgccgccg gctcagcctg cgtctccgga tggatcgtcg cctgacacca
     4741 ccacgcctca gcaaccgcag taaggaagcg cctcaaggat ttcagccacg catgtcgaac
     4801 ccatcggccc ccactccctc gcccaagagc atgctgcttc tctcgcggtt gctgctttcg
     4861 cccgcctttg ccgattccga tgagagaggc cggtccagtt tgcccggctc ggagctgctg
     4921 aacctgagcc gggagcactt tgaggaactg gtcgcgctgg ccagttcgaa ccatgtgatc
     4981 gtgcgcggca tggagaggta tgttgcggcg atgcgaagcc ggggcgatgc catgcgcgag
     5041 gagtgggcgc aggatgctct ggcgatggag cgcacgcgga tcgcgaatgc gattccgttc
     5101 ctgcgctcga tctgcgatgc gttcgaagaa gaaaaccagg atgtattggt aatcaagtct
     5161 ctggaccact ggcccgatct gggcagcgat ctggatctgt acacgccagc gtctccggag
     5221 acggttctgg aactgatgag acgccgcttt aacgcccagc tggctccgcg gagctggggg
     5281 gaccggctgg cgcgcaagtg gaacttcctg atcccgggat tgccggaggc ggtggagatt
     5341 catgtcggcc gcctggggca aaccggggag caaatggtgc tggcttcgtc ggttgcgagg
     5401 cgtgcgcgca agattcaggt cgatggcgtc ccgttccgga caacacatat ttcggatcgg
     5461 ctgatgatct cagcgctgca gcgcatgtat cgccacttct atttccgcct gtgcgacatc
     5521 attgacagcg ccgcgctgat tgagggcgag ctgattgact acgacgatct ggagcacgcg
     5581 gctaccgcag cgggcatttg ggaaggaatg gcaacctacc tcgtcatcgt tgccgattat
     5641 gtgagtttgt accggggtgc ggggccgcat ctgccgcaga cggtgacgtc caacgcacgg
     5701 tttggcggcg agaagatctt ttacgccaag gagttcctgc gggtgcccat cctgccgcag
     5761 tcggcagggc tctacggatc gcagctcggc gggctgttgc ggaagggcga actggaaagc
     5821 agcgtgcggc tgggtttgct gccctggctg gccggcgcgg ccgttgtggg tcaaaagatc
     5881 acgggcagcg ataagggaat ctggtaacgc cggtcgccct gttcagaact cgacttcgaa
     5941 cattgggcgg gaagagtacg aattcaaaag ggaagcttcg tgcgggccgc gcgacgactg
     6001 gctccggacc ggatcagctg agctcagtgc ggcatctccg tcgctggaga gctcgagtgg
     6061 cgcttcggtg tcgagtgcaa attcatcctg cagagcatgg ggagagggcg ggtcgtcgaa
     6121 cggatcgtcc ttcgaggccg gctgcatgcc attcaagtgc actctggccc cgttctccat
     6181 ggccttcttg cattgctgga gctcgaacca ccgcaggtaa ttgcggacga cgcaactcat
     6241 ggtcatgcgc acaccggcac gatagttgtg ctcggccatg cggcgctgca gttcggggga
     6301 gctgaggatc gcgatcagct cgtcggccag agcgttcgcg tcgccggtcc tgtaaaaggc
     6361 ggcctccata tcttcatcga tgaccatctc gcggaggtca ggaatgtcgg ctgaaacgat
     6421 ggggacaccg tattcgcagg cctgatgggc aggaccgctg gatccggtgg ctgagtcgta
     6481 cggcatgacg agggcggatg tggaggagta gagggcgggg atatcttcct cggccacgta
     6541 tccgagaaaa tcgatgggca ggtgagcggg ctgcgctgcg cggatcgatt cccaatagcc
     6601 ggcgcgagtg tgatgattgg ctccggcgat gacgagccgc gcattcggca ctttctccag
     6661 aactttggga aatgcctcca tcaaggtttc caggcgcttg taggttcccc agtgtccgat
     6721 ggcgaggatg cgctggtcgg gattaccgcg tttggagaag tcgggcggat cgggggtagc
     6781 ggcaaaggtg ccgtgtgtgc tgaggagcac attcttcgca gagtatttcg ccatgagcgt
     6841 gcgctgatac gcaggcagca ggacggtaac ggagtccgct ttgagaaggg cacgggtggc
     6901 gaagtcactg ccaagctgaa gcaagcggct cttacgaact ccggcagcat tgaagtcgac
     6961 gtgctcgagg atgtgatgga gagtgatgtg ggtaaagaag cccatggagc gaaccagagc
     7021 cggcgccgat agaccagcga aggcggcaac gggattctcg ggcgtggcaa aactggagaa
     7081 gaccaggttg aaccagacaa cgtcgggctt gatgcgacga atggtctgca gcagccgcag
     7141 cggggttttg aggctgccga agtcccagca gcggatcaca ttaaaaccgg gcagttcctc
     7201 ctgctgttgt ccgtcaaggg aatgtccatt gccatcggtg gcgaagtcat acttcgtcaa
     7261 ccgatcggcg agaatggtca gctcgacccc ggggtgacgc tgcaactcgc gcgcaatatg
     7321 gaatgcgtac tcgttgagct gccggccgct gggaggaaac gtcgcaacaa gacaaatctt
     7381 catcaaatcc cgcctcagaa caaggtgatg cttacgaagg gtctgaactg ctgactttgc
     7441 ccacccgtga aattgaagaa ggtctcctgt tgcactccgg cagaaaagcg gattggatac
     7501 tgcaggacca catctccggt ttggtctcga aattcccggt ggaaaggcat ggcgtaagaa
     7561 acggaaagat tgctctgtac cgcgtcgtag acatgaaagc tgcgattgct ggaccaggct
     7621 gcgccaccct ggaggctcca gcgcggtgtg actctgaagt tgaccgtgcc cgccggacgc
     7681 aggacctggg cgatgccgga gcgcccctgg aaggtacgcc aggcgcgcag atcttcgacg
     7741 atgcctttca cctgggcgcg ggtgccgaac tggcggtcga ggccgatgta tgagccggtg
     7801 taatagtcct cggtggagac gggatcaaac ttctggtggt tttcgcccca gccggtgatg
     7861 agggcggttc tgccccatgg ggcaccgacg cggaagttta cagcggcgga gaacgcggtg
     7921 gagtgaatgt tggattcggt gaacgggccg gtctcgcgca gaacgaagcc atccatggag
     7981 acaacgttga agaaggaact ggtggacaca tacgtgaaga gccggaacag gttctggttc
     8041 aggtccactg gatcctcgga atcacgccgc acggtcgcct ggataccgct gttgaaggtg
     8101 atgacgttat ttccgatccg tgccgttggg ttcactccga agttgaagcc gtaatccgtg
     8161 gtattgcggt tcacgatcga acttgtcgcc ggaaccgaga tggtgccgcg agcgttgcgt
     8221 acctggaaaa agccgctcgc ggggggcaag tagttgagat gcaaatgaaa ggcgttggtc
     8281 tcctcggtct gcagcgaaga gcgaggcggc ggcagcaggg aagtgtcggt gggagcgacg
     8341 gggaccggcc catccagttt cgaatccagc acatagacgg atgtgtcttc gaagatgggc
     8401 gaaaccatgg catcggcaag gacgctgaag tggttattca gtctgagtcc ctcgtcggcg
     8461 ccggtttgca acagattctg ttcggcggtc tgatcttcgc cggcggcgtc ggtggcctgg
     8521 gcgaaagcgg tgagcgcctg ggggttccga tgttcctggc ggtagacgct ggctttggcc
     8581 aacagatact ggtagtccgg ctcttcgtcc ggcgagttgc tgatcgctgc tagttcgccc
     8641 tgggcacgag gggtgtctcc caatgcgagg tacgtattgg ctagtccgac gcgcacagca
     8701 gtatcggatg caccggcggc ctcggcccgt tggaggtaat cttccgacaa ttggtaatca
     8761 tgcatctggc gaaacaggtc gccgacctgg atcatctgcg ccccggtggg gagctttgcc
     8821 tcgcctgact cagcctccat gagggagagg gcgatttcgc ggcgcgcatc ctgggtctgg
     8881 ttcttctgtg ccatcacttc tgcaatcgcc aggcgaacgc cgacccggtc gctgcccggg
     8941 agagtgagcg cgcgccggaa gcgttccatg gcggcagcgt gatcgccaac ggtattcaga
     9001 gactcgccgg tgagcaggaa gatctgcgcg gcgtcggatt cgcctgggga cttcgccgac
     9061 gccggcttga gcatcgcgcg ctgttcggca atcttgacgt actgcatggt ttcagcggga
     9121 ttatcgagtt cggcccacga gcgcgcgagc cacgcgtaga tcatggcgtt gtcaggagca
     9181 agcttttgcg cgacgttcag ctcatcgacg gcgtcgcgga aacgacgctc ggtatagagc
     9241 acgttcgcca gcgccaggtg gagcgatccg tcgttgggcg tgtagcgaat ggcggcgcga
     9301 tattctctgg ccgctttcgg caatgcgcct tgttgtccgt aggcggtggc gcgaaccaag
     9361 tggatcgcct gactttcgcc aagagcgctt tccgcaagcg caacctggcg cagtgccaga
     9421 cgcggcttgt ggagatcgag gctggtgtac gccagaccga ggtgggcttc gccgtccttc
     9481 gaatcgcgct tcagagccgc ttcgaagtcg gcctccgcag cctgaggctg gtggagatcg
     9541 tgcaggacat agccacggtt gacataggcg gtgacgacgg aaggatcgcg ctggagtgtc
     9601 tcggtgaaag cctggcgagc ctcttccagc tgattgttgt gcttgtacac gtaaccggcg
     9661 aggagaggga tgtccggctc cttcgggagc accgcatcgt attttttggt cagggcgagc
     9721 agatcgtcgt agcgattgag gtggaggagg tcgtacccca tgtagacgac cacatctctg
     9781 tcgttcggca gcccctgaat ggcgcgttcg cctttggcaa gggactccgg atactgaccc
     9841 ttgaagctga ggtagcgttc ctgctcgcgg agaacctggg gctcgtcctc catgtctttg
     9901 gtagcgcggt tgaaccaggt tgcggcggca tccagcttgt ggccttcgat atccgcgttc
     9961 aacccgccgg cgacaacaag agcgttgtca ggagcgaggg cataggcttt cgcgtaatca
    10021 gcctcggctt tcgtgtattc cttacgcgag gcgttcatgt cgcccatggc gagccaggga
    10081 acatagaggg tgggataggc ctgggcgagg cgctcgaaat atttttcggc atcctgatcc
    10141 cggccggccg cccgcgacgc gtagccgatg gaggtgagcg cgaagcggtt ctttggatcg
    10201 atggtgagga tctttttgta gacggcgatg gcttcttcgg tatggcccat cttgacgagc
    10261 agatcgccat cgagctgcag gctgttggga tcggtcgcat tgatggcgag ggccgcgttg
    10321 aggtcgctgc gggctccggt gaggtcgccg gtgtccatgc gaatcatcgc gcgcagccgc
    10381 aagaacgggc cgcggtctgt accgttctca tcgagggcgt tgatggcggc ctgcgcggtt
    10441 ttgagttgct ggtccgcctg atctggctga tgggtgttct tgtacagctc catgagatcc
    10501 atgtggagct ggatggggta aagagggcct tccacggggt cggcgggaat atgcgccagg
    10561 gcggcctggt attcgcggac ggcgtcgttg tctttctcct gaatgcgcgc gatctctccg
    10621 cgcagggcat gcagacggta gtgttccgga tcgattgctg ctgcccgcgt aaggaacgta
    10681 tcggcgttgt ggatggaacc gaccgcgact tcggcctggg ctgccgacaa ctggaggttc
    10741 agatctttcg ggacggcatt ggcgacctga acgtacagcc gggcggcctc ctcggcgttg
    10801 ccgtcgagct gataggtgta ggccagctcg gctttgatgt cggggcgctg cgacttgatg
    10861 gatttcagcg ctgcaatggc ttcgtcataa tggccggtct cacggtagta gccggccatg
    10921 gcgcgctgga cttcaggatt attcggcgcg cgtgctttgg ccatttcgag atagtgacgg
    10981 gactggtcga gcttattctg gtgctgatac gccatggcca gcagcaattc ggaacgggcg
    11041 ttgccctgga aacgctcggc ctgatccaga gtttttacgg catcgtcata attgccggag
    11101 cggatctgca ggtcgcccag caggacggca tcgtcgatct gctttgggtc gacggcgagc
    11161 actttctgca gcatgtcctg tgcttctttg gtgcggccca tgacggcgta ggtctgcgca
    11221 aggccagaga gtccgagaac cgaagagggc tcgaggcgaa ggccgtggtt gtaggcgtcg
    11281 acagcgaggc cggtttgcgc gttcaggcgg gcggcataac cgagcaggaa ccagagctgt
    11341 ggattatttg gggccgactg cgcggcacgc tgggcgtagg tgacggcctg ctggtgatcg
    11401 ccacgcttca atgcggcttc ggcagcgccg gcgagccggg cattctggat attggagccc
    11461 catcccagct gcttttcctg ggattgaggc tgggtggcag cgggttgggt cttgttggat
    11521 tgcgacttcg tgttgtcgtc ggaaccgacc ttgtacgtct gggcaaaaac tggtccgcta
    11581 aaaagaagaa ggaacagaag aactctggca attcgggggc tttcaggtcg aacaattggc
    11641 atggcaccag tgactcgcac ggaataccgc cttggatgcc caactggcgg tgaacgttgg
    11701 ccttaagaat gcaactgagt ggctgttctg agacaacacg atcgccaatt ttcgaatcca
    11761 atgcaatttc gtgaggattc cttgcaaaaa atagagactg tcgcgccgtc ttctcggccc
    11821 atccccatga gttttattct gcttgcggtt tttgcggtgc atctgccgct gctgctcatg
    11881 gaattgcctt tgaagtcgta cgacacgaac ttcaatatct tcttcgcatc gcattacgtg
    11941 catcactggt tcgatccgtg gaatgcgaag tggtatgcag gattttcaca gacgacgtat
    12001 ccgccgttgc cgcagcagtg ggtcgctgtc gtttcgtggc tcaccggcct gaatcttgcc
    12061 tatatgacgg tgcagtttgt cggcattctg ctgctggtgg tgggcgttta ccggtttgcg
    12121 aagctgtggg tcagcccgcg ggcggcatcc ttcgcggcac tggcgaccgt ttttctgggc
    12181 tcggaagcct tcctgattta ctcagcaggg cagctgtcga ccacctgcgc cgcgccgttg
    12241 tatctgaacg cgttgccgta cttgtttgag tggattcggg aagggaaggg gcgatcattc
    12301 ctcaaggggg ctgtgctctt cacggcggcg gcggcggccc accatgccac gttgttgttc
    12361 ggatcgatgt tctttgccat cccggttgtc gctcttgcgc tcattgacaa gcgcggcgac
    12421 gagccggtgc ccaggggtgc gatcatcggc cgcacgatcg cgatcacgat cgtggtgggc
    12481 gttgccgttg ccattgtgct gctgccgttc tggctggcgc tgattcacta tcccgtgaca
    12541 cagacgccga ttccccatgc gagccgcgcg aactacatcc tgagcccgca gtggggtgtg
    12601 aactacttca tcgttccgtg gggcgcgttg atccttgcgc tgccattcat cttctggcgc
    12661 ggagcatcgg tgccgcgtct tcgaccattg ctgatcggct tctgggtcgc gtttctgtgt
    12721 ggactgggcg gcacgacgcc cgtagggcat tggctactgg gtcgcgcctt cgaggtgctt
    12781 acgttcgagc gtttcagcta ttgggcgacg ctgctggctg tgccgttcgt aggactgctg
    12841 gcctcggaac tggtggatcg gtttcgcttt cgggcgatag cggggcttgc ggtgctggcg
    12901 gccgcaacct gcgctctggc tgtatcgtgg tcgacatttc atccggccga aggcgaagaa
    12961 tttcgcgtgg atggggttgc gaactggctg aaccgtgacg gtcacgataa ttaccgttac
    13021 attacgctgg gttttggaaa ccagatttcc cgtctggcga ttctgacgga tgctggcagt
    13081 ctggacggcg aatggaactc cggccgtatg ctgccggaac tgcaggcaca cggcggcggc
    13141 gcgctgacca gcgcgaagtt ttttggcaag gatggcctgg actcgctgca tgcgatcctc
    13201 gaccacgcgg atcgctatgg tttgcggtgg gtcttcgtgc gcgatccgtt ctacgatccg
    13261 ctgctcgcct ttggcgggtg gcgccatgtg gatgaactga atgcgggcac gattgcggtg
    13321 tggaccaaag atggcgttcc gccggcgacg ccggtgaatg cgccgcagat gcctccggca
    13381 tggcagggaa tcatgtgggg aacgctgccg ttcggcagca gccttctggc gattctc
//
LOCUS       DASZYN010000338         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_848755, whole genome shotgun sequence.
ACCESSION   DASZYN010000338 DASZYN010000000
VERSION     DASZYN010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848755"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VHE33_08780"
     CDS             complement(<1..395)
                     /locus_tag="VHE33_08780"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVT97590.1"
                     /translation="MKRIAIIGGGIAGLSAAWTLEKTRRANSGNAEALEYHVYEAADR
                     LGGVISSETIDGCVVEGGPDSFLTEKPAAAALCRELGIADQLLPSNDAERKTYIVVKN
                     RLIPLPDGLMFMVPTKLVPTAFTRLFSWST"
     gene            complement(392..1627)
                     /locus_tag="VHE33_08785"
     CDS             complement(392..1627)
                     /locus_tag="VHE33_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04053 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HVT97591.1"
                     /translation="MDISQAMQHLDYNRAPFLAIWETTQACDLACKHCRASAQPQAHP
                     SQLNTMEATSLIDQIADLEVPLFVFTGGDPLKRPDIFQLIEHAAKRGVHAALTPSATP
                     LLTRDAIFRMKASGLSRLAVSLDGSVPDIHDSIRGIPGTWQRTLQAIEWANEAKQPIQ
                     VHTVVSRLNIGDLDNLSVLLTVKQIVMWSFFFLVPVGRGQVADLLTGQEFEDVFEKMW
                     DLTKRVPFAIKTTEAMHYRRYLIQQRMKEGLATTGVHDDAGARPTRHPGWTVSDGHPG
                     RGSEAKAVGWATKRVNDGRGFVFISHIGKVYPSGFLPIEGGDLHNDTLAHIYQESPIF
                     VKLRDSDQLHGKCGACEFRNICGGSRARAYAVTGDVMAEEPCCIYQPKGYHPDPNQKT
                     ASTPGCEAHNLIQLEVAHS"
     gene            complement(1702..2352)
                     /locus_tag="VHE33_08790"
     CDS             complement(1702..2352)
                     /locus_tag="VHE33_08790"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HVT97592.1"
                     /translation="MPLHLSPVEARVLGSLIEKETTTPEYYPLSVNALVNACNQKNNR
                     EPVMQLNEDEVRTALNRLRDQNLAAAVHDSRVTKYEHHLQEVLNLTRGETAVLCVLLL
                     RGPQTPGELRGRTERMHRFEELEDVLAVLDKLAQHDPALVVALPRQAGSREIRSAHLL
                     SGQPDLSQIATPPPESSPVRSSDESRIAQLEAEVASLREQMAELKVQIAALSMARS"
BASE COUNT          518 a          769 c          818 g          503 t
ORIGIN      
        1 gtcgaccacg agaaaaggcg cgtgaacgcc gtgggaacca acttcgtcgg caccatgaac
       61 atcaggccgt cgggcagagg aatgagccgg ttttttacca cgatgtacgt cttgcgctca
      121 gcatcgttcg acggcagaag ctgatcggca atgcccaatt cacggcagag cgcagccgct
      181 gcaggttttt ctgtcaggaa cgaatcggga ccgccttcaa cgacacagcc atcaatcgtc
      241 tcggaactga tgaccccacc cagtcgatcg gccgcctcat agacgtgata ttccaaagcc
      301 tcggcgtttc cggagttcgc ccgccgagtt ttttcgaggg tccacgcggc gctgaggccg
      361 gcgatgcctc ctccgatgat ggcgatgcgc ttcaagaatg ggcgacctct aactgaatca
      421 gattatgcgc ttcgcatcct ggagtggaag cggtcttctg attgggatcg gggtggtagc
      481 ccttcggctg atagatgcag cacggctctt ccgccatcac atcgccggta acggcgtaag
      541 cgcgcgcccg tgagccgccg caaatgttgc ggaactcaca cgcgccacac ttgccgtgga
      601 gctggtccga gtcgcgcagc ttcacgaaga ttggcgactc ctgatagata tgcgccaacg
      661 tgtcgttgtg caggtcgccg ccctcgatgg gcaggaatcc gctcggatag accttgccaa
      721 tatgcgagat gaagacgaac ccgcgcccat cgttcactcg cttggtcgcc cacccgacgg
      781 ccttcgcttc cgacccgcgc cccggatgcc cgtcgctgac ggtccagcca ggatgacgcg
      841 tgggccgagc gcctgcatcg tcgtggacac cggtcgtggc caggccttcc ttcatgcgct
      901 gctgaataag gtagcggcga tagtgcatcg cctccgttgt cttgatcgca aagggaacgc
      961 gtttggtcag atcccacatc ttctcgaaga cgtcttcaaa ctcctggccg gtaagcagat
     1021 cagccacctg gccccgaccc accggaacga ggaagaagaa gctccacatc acaatctgct
     1081 tcaccgtaag cagcacgctc aggttgtcaa gatcgccgat gttgagccgg ctcacaacgg
     1141 tgtggacctg gatcggttgc ttcgcttcat tcgcccactc gatcgcctga agagtgcgct
     1201 gccaggttcc gggaatgcct cggatcgagt catgaatatc aggaaccgac ccgtcgagcg
     1261 acactgccag acgtgaaagc ccggaggcct tcatgcggaa gatagcgtcg cgagtcagca
     1321 gcggcgtagc gctgggcgtg agtgcggcat gaacgccgcg tttcgccgca tgctcgatga
     1381 gctgaaaaat atcgggccgc ttcagtgggt caccgccagt gaagacaaag agcggaacct
     1441 caagatcggc aatctggtcg atgaggctgg tggcctccat cgtattgagc tggctggggt
     1501 gcgcctgggg ctgcgccgaa gcccggcagt gcttgcaggc gagatcgcat gcctgggtgg
     1561 tttcccaaat cgccagaaac ggcgcgcgat tgtagtcgag atgctgcatg gcctgggaga
     1621 tatccatggc aacaggttgg cagggggcgg gcgggcgcgg atgtgacttc catcacaggc
     1681 tgcaaatcca gcgtcaggag ctcatgagcg ggccatcgaa agggcagcga tctggacctt
     1741 cagttcggcc atttgttcgc gcagagaagc cacttcggct tcgagttgcg cgatgcggct
     1801 ctcgtccgaa gagcggacgg ggctggattc gggaggaggc gtcgcgatct gcgagagatc
     1861 gggctgtccg ctcagcaggt gcgcggagcg aatctcgcgc gaaccggcct ggcgcggaag
     1921 ggccacgacc agcgcgggat catgctgcgc caacttgtcg aggacggcga gaacgtcctc
     1981 cagctcctcg aagcgatgca tacgctcggt ccgtccgcga agctctccag gcgtctgcgg
     2041 tccgcgtaaa agcagcacac agagcacggc ggtttcaccg cgcgtcagat tcaggacctc
     2101 ctggagatgg tgttcatatt tcgttacgcg gctatcgtga acggcggcgg caagattctg
     2161 atcgcgcagc cgattcagcg ccgttcgcac ctcgtcctcg ttgagctgca ttaccggctc
     2221 acggttattc ttctgattgc acgcattcac cagcgcgttg accgagaggg gatagtactc
     2281 cggcgtagtg gtttccttct cgatcagaga accgaggacg cgagcttcga cgggggagag
     2341 gtgaaggggc ataaaaggag ttcagcctga cgggagaatc gctgcggacg atctgagttt
     2401 acggcatggc ccgtatccgc ccaggcaccg ccgtgaaccc tcccaccggg gtggtatgca
     2461 ttgttcacgc tataataaag aacgtgagca ccgtcattac cgagacacct gtcgctcccc
     2521 agcgtgcggc gtccggaccc ggggaaaagc gggtcttatt gctgaaaccc cgtggttttt
     2581 gcgcaggcgt ggtccgggcg atcgatat
//
LOCUS       DASZYN010000339         8021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_15824, whole genome shotgun sequence.
ACCESSION   DASZYN010000339 DASZYN010000000
VERSION     DASZYN010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8021
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15824"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VHE33_08795"
     CDS             complement(<1..429)
                     /locus_tag="VHE33_08795"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HVT97593.1"
                     /translation="MATETYHSELKERSGVPQPPEPPASAGKDEISLLDLLIIVAERK
                     MTVLYVTAAVAVVSVIVVLLLPSWYTAEVTLLTPQQNSSLGSAIASQLGSLGPMAALA
                     GAGGLGLKSPNDMYVAMLKSQTVEDGMVQRFHLESDYKEKR"
     gene            complement(496..2931)
                     /locus_tag="VHE33_08800"
     CDS             complement(496..2931)
                     /locus_tag="VHE33_08800"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HVT97594.1"
                     /translation="MPLSLGQTPQTSPQRPQFFDYRDNENLARQPATRQPPQTPLAPE
                     GLTEFQSFVASSTGQVLPIYGTDLFQHVPSTFAPLDMVPVPPDYVIGPGDELRVRVWG
                     QVNFSANVRVDRSGEIYVPQVGQVHVAGMPFSALDEHLRSAVSRVYRNFDLTVDVGQI
                     RAIQVYLAGSARSPGVYTVSSLSTLVDALFAGGGPSASGSFRHVILRRAGAEVTDFDL
                     YSLLVHGDKSKDVTLQNGDVLFIPPAGTQVAVTGSVKIPAIYELRAGETLGDALADAG
                     GGSVVAAHARVSIERIDEHRDRSTMEVAWDSAGLATPLADGDLVRVFSIIPRYQKTVT
                     LRGNIANPGRFAWHEGMHVSDLIPDKDSLITRNYWWKRAQMGLQGPEFEPLQHFPPLK
                     QPTEATDLPRRRLLEPSMAGQSQQTENGQSAGAGYGTPNDPNNPNYQNDQIGAGGAAG
                     QQGGMGQRNLAASQRAGSASLAVEQPRSTAVPAVRTEVGPVAPEIDWNYAVIERMDRT
                     TLKTSLIPFDLGRLVLQHDTSQDLALEPGDVVSIFSQNDIRVPIAQQTKFVHMEGEFV
                     HSGVYTVQPGETLRDLVRRAGGFTASAYLYGSEFTRESTRAIQQRRMDEYVQELEMQM
                     QRGNLAVAAAGAGTPQELASGQAARSSEQELLGRLRQMRATGRIVLEFRAGSSGIDAL
                     PDIALEDGDRFVVPPVPASVNVVGAVYDQNSFLYETGRSAGTYLRLAGGSNRDADRKH
                     MFVIRADGSVISRAAENGIWGNQFDNLRMNPGDTIVVPNKTFKPSILRGILDWSQLFS
                     QFALGAAAINVLH"
     gene            3559..4404
                     /locus_tag="VHE33_08805"
     CDS             3559..4404
                     /locus_tag="VHE33_08805"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HVT97595.1"
                     /translation="MRLGSTDRSAKIPGSRTASSHAAASDFVTPVPASAWALSAFRRT
                     IEIAVAVIALIVFSPLMFLAAVLVRCGSAGPVFFRQRRMGRHGHEFTLYKFRSMCPRK
                     GNNSSITVSGDVRITPVGGFLRRYKLDELPQFWNVLRGDMSLVGPRPKLPHHEALRLV
                     CRPGITGAATLAFRNEEEFLSEIPEDQLETFYETFVKPAKAHLDMEYMRTATLRSDVK
                     ILWRTASACLFSTDDSAADSAATIMQYEEAARIRAEKLPSLSVGHSKPKPIAYPVAAG
                     ANQEL"
     gene            4642..>8021
                     /locus_tag="VHE33_08810"
     CDS             4642..>8021
                     /locus_tag="VHE33_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HVT97596.1"
                     /translation="MGSRIQAPGLLPGAVVWHDRSSLSSRTPRRHNHGVLLPVLLLLG
                     MAAVCPSLRAQPSTQLKAAPADIPVPSTVWKSIGPTQVSTSAWNLVSGRVISIAADPS
                     DSTGNTLYLGTEGGGVWKSVNAAASPASVTFIPLTDNLSVWSSSVPTSLSIGAVSVQP
                     GGTGVILAGTGNPDSGTGSWYGAGFLRSTDNGNTWSLTWMTAAGPFGGSFNLLGTAVA
                     GFAWSTTNPGLVVAAVSEPGNMTTIANAFTKVNFPGIYYSQDAGATWHLATLEDGTQV
                     IQGERYISNVGNAATAVVWNPIRKRFYGAVRYHGYYESPDGVTWTRLVNQPGSSLTTV
                     LCPTNPGSAPSPACPLYRGALAVQPSTGDMYALFVDSNNLDQGLWQDICQLKSGNCSS
                     STARFTTKIDDKPLQSPAGSGTLAQADSGLWLAAVPSQQDTLLFVGDQDIWRCSLAKS
                     CVWRNTTNTQTCTAAHVTPGQHAIDATFGSTGLLWFGNDAGLWRSTDAVSQSKTSCSS
                     DDAAHFQNLNGSLGPLAPVASFAEDPLNPSTWLAALGPLGTAAPGADMTAPWSQILNG
                     EGDNVAIDPASPSNWYATSIGGVGINRCTEGTACDISGFGSVAIGETQVLNDFQSVPA
                     PWILDPQNSANIILGTCRVWRGPATGIGWSQQNLLSTMLDKVRQPYCDGNSAISSIAA
                     APITSGALAGNGAEQLYVGMAGALDGGGLIPGHVLTAVINNDSQSATTTWIDRYASPV
                     TNQGITAGQFNPQGYGISSIVPDPHDPTGQTVYVTLRGISGTSSFIPVVYRSTDAGAH
                     WANIGSNLSSTPANSFLVDPNDANIAYIATDAGVFFTQKISSCLSMNSACWNVYGSGL
                     PTSPATSLMAYNQGATQKLRAATWGRGIWEADLATAGIAPTTATVEPSSLTFSGQQVQ
                     TTSAPQTITITNAGNLNLNVSSVDITGDFTETDNCIDQSLDPHASCQIAVVFAPSQTG
                     PASGLLTLYANLAGGQLTVPLSGTGLAPASLRLTPPSLTFPGTTVASRSDPEYVTLAN
                     TGDIAASLAGETITGDYSIAANTCGASIAPNSSCTLGIVFTPTASGTRTGILTVSDSV
                     GIQTLPLSGSGQSPPTDTLSSLSLNFAPQQVGTTSTAQP"
BASE COUNT         1498 a         2783 c         2149 g         1591 t
ORIGIN      
        1 acgtttctct ttgtagtcgg attcgagatg gaatcgctgc accatgccgt cttcgaccgt
       61 ctggctcttc agcatggcaa catacatatc gttcgggctt ttcaggccga ggccacccgc
      121 ccccgccagt gccgccatgg gaccaagact gccaagctgc gatgcaatcg ccgagcccag
      181 cgaagagttc tgctgcggag tcagcagcgt cacctccgcc gtataccagg acggcagcag
      241 caggacgacg atgactgaga ccactgcaac ggccgcagtg acgtaaagaa cggtcatctt
      301 gcgctcggcc acaatgatca gcagatcgag cagcgaaatt tcgtccttgc ccgccgaagc
      361 cggaggttcc ggaggctgag gaacgcccga gcgttccttg agttcggaat ggtatgtctc
      421 ggttgccatc aaaaagccgc tgcaaatccc gaactgctgc tgtttatttt acagactgat
      481 gccgcaccca cccggtcaat ggaggacgtt gatcgctgct gctcccaggg cgaactgcga
      541 gaacaactgc gaccagtcga gaatccctcg caggatcgac ggcttaaagg tcttattcgg
      601 aaccacgatc gtatcgcctg gattcatccg gagattgtcg aactgattcc cccagattcc
      661 attttccgcc gcacggctga tcacagaccc gtccgcacgg atcacgaaca tgtgcttccg
      721 gtcggcatcg cgattggatc cacccgccag gcggagataa gtgccggcgc tccgccctgt
      781 ctcatacagg aacgagttct gatcgtagac cgcacccacc acgttcacac tggccggtac
      841 gggaggcacc acgaatcggt ctccatcttc cagtgcaata tccggaaggg catcgattcc
      901 gctgctgccg gctctgaatt caaggacgat ccgtccggtc gcccgcatct gccgcagccg
      961 gccgagtagt tcctgttcgc tggatcgcgc cgcctggccg ctggccagtt cctgcggggt
     1021 accggctccg gcggcagcca ccgccagatt cccacgctgc atctgcattt ccagctcctg
     1081 cacgtattca tccatgcgcc tttgctggat cgcccgcgtc gattcgcgcg tgaattccga
     1141 tccgtacaga taggcgctcg ccgtaaaccc tcccgcgcgc cgcaccaggt cgcgcagggt
     1201 ctctccaggc tggaccgtat acacgcctga atggacgaac tcgccttcca tgtggacgaa
     1261 cttcgtctgc tgggcaatag gaacgcgaat gtcattctgc gagaagatcg aaaccacatc
     1321 cccgggttcc agcgcgagat cctgcgaggt atcgtgctgc aacacaagtc tgcccagatc
     1381 gaagggaatc aggctcgtct tcagcgtcgt ccgatccatc ctctcgatca cggcataatt
     1441 ccagtcgatt tccggggcca ccggtcctac ttcggtgcgc actgccggca cagccgttga
     1501 cctcggctgt tccaccgcga gcgacgcact gccggcgcgc tgcgatgcgg ccaaattccg
     1561 ctgccccatc ccaccctgct gtcctgccgc cccgccggct ccgatctgat cgttttggta
     1621 gttcggatta ttcgggtcgt tcggcgtacc gtagcctgca cccgccgact gaccattctc
     1681 cgtctgttga gactggccgg ccatcgatgg ctcgagcagc cgccggcgcg ggagatctgt
     1741 cgcctcggtg ggctgtttca gcggtggaaa atgctgcaat ggctcaaact ccggaccctg
     1801 caaacccatc tgcgcccgct tccaccagta gttgcgcgtg atcagcgagt ccttgtccgg
     1861 aatcagatcg ctcacatgca tgccttcgtg ccaggcaaaa cgtcccggat tcgcgatatt
     1921 tccgcgcagc gtgaccgtct tctgatatcg cggaatgatc gaaaacaccc gcaccagatc
     1981 gccgtccgcc aaaggcgtcg ccaacccggc cgaatcccat gcaacctcca tcgtcgatcg
     2041 gtcgcgatgt tcgtcgatgc gctcgatcga gacgcgcgca tgtgctgcca ccacggatcc
     2101 gccaccggca tcggcgaggg catcgcccag tgtctctccg gcgcgcagtt cataaatggc
     2161 cgggatcttc acgcttcccg tgaccgcgac ctgcgtgccc gccggcggaa tgaagagcac
     2221 gtcgccgttc tgcagcgtta cgtccttcga tttatcgcca tgcaccagca gcgagtagag
     2281 atcgaaatcc gtgacctcag ctcccgcccg gcgcaaaata acatggcgga acgaaccgct
     2341 cgccgaaggc cctccgccgg cgaacagagc atcgaccagc gtgctcagcg aactcacggt
     2401 atagacgcca ggactccgcg cagaacccgc caggtaaacc tggatcgccc gaatctggcc
     2461 cacgtcgacg gtcagatcga aattgcggta gacgcggctt accgcggagc gcagatgttc
     2521 atccagcgct gaaaatggca ttcccgctac gtggacctgg cccacctggg gcacgtaaat
     2581 ctctccggag cggtccactc gcacgttggc gctgaaattc acctggcccc agacacggac
     2641 gcgaagttca tcccctgggc cgataacgta atcgggagga acagggacca tgtccagcgg
     2701 tgcgaacgtt gaaggcacat gctggaacaa atccgttccg taaatcggaa gcacctgtcc
     2761 ggtactcgac gcgacaaagc tctgaaactc ggtcagtccc tcgggggcca aaggtgtctg
     2821 tggcggctgc cgcgtggcgg gctgtcgagc gagattctcg ttgtctctgt aatcgaagaa
     2881 ctgcggccgc tggggactgg tttgcggcgt ttgcccaaga ctcagaggca tctgtccgga
     2941 ttcctgcatc gtacattgtg acgagttcgc caggagcggg tccgtgcagt cgacaccgga
     3001 agaagagttc tgcgggttgc cgggaagctc ctgcgcagcc gcagacacca tggcaaacag
     3061 gcacgccgca gccagtactg aggggactag tttcatcata cggaggtaat gcacgaagtc
     3121 ggaaggggac gcagagtgcg ccgcgaagct tttggtttca ttgattcgtg cgaggaagaa
     3181 ttcctgtgac cacagtctag cacgcgctct gccaggtatc gtcgcgtata cctttggggg
     3241 attgatatgc gcctttggtg gacgttattg tcgtaatgat cttcgcgtga catccgtgcg
     3301 cgcatgatcg attttgatcc ttgatctgca ggcctgcacg gtatactgca cgaagaaaca
     3361 ccacagcctg ttgattacaa tttagtaaca acttagacat tcatacgtct ccatcttcag
     3421 gacccgccgg aacactgatt ttttccctct ggtttgcgaa agtcagaatg ccaacgttgt
     3481 ttgtgatggg tgggaatgag ttcgctttca ttcgataatg tgttttcttc cgacacctct
     3541 gatacgtcag caggtgccat gcgccttggc tcgaccgatc gtagcgcaaa aatccccggt
     3601 tcccgcaccg cttcctctca tgcggcagcc tccgatttcg tcactcctgt acctgccagt
     3661 gcgtgggccc tgtccgcttt ccggcggacg attgaaatcg cagtggccgt gattgccctc
     3721 attgttttct cgcccctcat gtttctcgca gcggttctgg tccgctgcgg ctctgccggg
     3781 ccagtcttct tccgccagcg tcgcatggga cgccacggcc acgagttcac cctttataag
     3841 tttcgttcca tgtgcccccg caagggcaac aactcctcca tcaccgtcag cggtgatgtc
     3901 cgcattacgc ccgtcggggg tttccttcgg cgttataagc tcgacgagct cccgcagttc
     3961 tggaatgtcc ttcgcggcga catgtcgctg gtcggtcccc ggcccaagct gcctcatcat
     4021 gaagctctgc gtctggtttg ccggcccggc atcaccggcg ctgccacgct ggcctttcgc
     4081 aacgaagagg agtttctctc cgagatcccc gaagatcagc tcgagacctt ttacgagacc
     4141 ttcgtcaagc cggccaaagc ccatctcgat atggaataca tgcgcaccgc aaccctgcgc
     4201 agcgatgtga agattctctg gcgaaccgcc agtgcctgcc tcttttccac cgacgattcc
     4261 gctgccgatt ccgctgcgac catcatgcag tacgaagaag ctgcccgcat tcgcgcagag
     4321 aagcttcctt ccctttcggt aggccattcc aaacccaagc ccatcgcgta tcccgtcgct
     4381 gccggcgcca atcaggaact ctaagtaagc cctcatccgc gcgttccctc aaaggggacg
     4441 tgcgggtgcg cgtcgcagca ctccgcattt ttcccaattc gaacctgtcg cggtactacc
     4501 ggcaccaatg caaactgaag tgagtgccgc ggcccccatt tcgctccgaa taataggcct
     4561 atccgcccgc tgactcgtgc gcgctatcgc gttgcacact gccgtcgagc ggaaacctga
     4621 cggttatcgc ggtgaacctc tgtggggagt cgcatacagg cacccgggct tctccccggc
     4681 gccgttgttt ggcatgatcg gtcatcgctg tcttcgcgga ctccgcgccg ccacaatcac
     4741 ggtgttctgc tgccggtgct gctcctcctc gggatggccg cagtgtgccc gtctcttcgc
     4801 gcgcagccgt ccacccagtt gaaggcggcc ccagcggaca tccccgttcc ctccacggtc
     4861 tggaagtcga tcggtcccac ccaggtcagc acttcggcat ggaacctcgt ctcggggcgc
     4921 gtcatcagca tcgccgctga tccctccgac tccaccggca acactctgta cctcggcact
     4981 gagggcggcg gagtctggaa gtccgtcaac gcggccgcca gtcctgcgag cgtgactttt
     5041 atcccgctga ccgacaatct cagcgtctgg tcctcttctg ttcccacctc attgagtatt
     5101 ggagctgtct ccgtacagcc tgggggaacg ggcgtgatcc tcgccggaac cggcaatccg
     5161 gacagcggta ccggctcctg gtacggcgca ggctttctgc gctccaccga taacggcaac
     5221 acctggagcc tgacgtggat gacggcggca ggtcccttcg gcggcagttt caacctcctc
     5281 ggtacggccg ttgccgggtt tgcctggagc accacgaatc ccggcctcgt cgtcgctgcc
     5341 gtcagcgagc ctgggaacat gaccaccatc gccaatgcct tcaccaaggt caactttccc
     5401 ggtatctact actcgcagga tgccggcgct acctggcatc tcgcgactct tgaggatgga
     5461 acccaggtca tccagggcga gcgatacatt agcaacgtcg gaaacgccgc cacggccgtc
     5521 gtctggaatc ccatccgcaa gcgcttctat ggcgccgtcc gctaccacgg ctactacgag
     5581 tcgccagacg gcgtcacctg gacccggctc gtcaatcagc ccggatccag ccttaccacg
     5641 gttttgtgcc ccaccaaccc cggttcggcg ccatctcccg cctgtcccct gtatcgcgga
     5701 gccctcgccg tgcagccttc caccggcgat atgtacgccc tcttcgtcga cagcaacaat
     5761 ctcgaccagg ggctctggca ggatatctgc cagttgaagt ccggcaactg ctcttcctcc
     5821 actgcccgtt tcactacgaa gatcgacgac aaacctctgc agtcccccgc cggcagcggg
     5881 actctcgccc aggccgattc cggcctgtgg ctcgctgctg tgccctccca gcaggacact
     5941 ctcctgttcg tcggcgacca ggacatctgg cgttgcagcc tggcaaaaag ctgcgtctgg
     6001 cgcaatacca ccaacacgca aacctgcacc gccgctcatg tcaccccagg ccagcacgcc
     6061 atcgacgcca ccttcggatc gaccggtctc ctctggtttg gaaacgatgc gggcctttgg
     6121 cgatccaccg acgccgtcag ccagtcgaag acctcttgct ccagcgacga tgccgcccat
     6181 ttccagaacc tcaacggcag ccttggccct ctggcgccgg tcgcctcctt tgccgaagat
     6241 cccctcaacc cgtccacctg gctggcagcg ctcgggcctc tcggaacggc tgctcccggc
     6301 gccgacatga ccgcgccatg gtcccagatc ctcaacggcg aaggcgacaa cgtcgccatc
     6361 gatccggcga gcccttcgaa ctggtatgcc acctccatcg gtggcgtagg catcaaccgc
     6421 tgcaccgaag gcacggcctg cgatatcagc ggtttcggtt cggtcgccat cggcgaaacc
     6481 caggtcctga atgactttca gtcagtccct gccccatgga ttctcgaccc gcagaactcc
     6541 gccaacatca tcctcggcac atgccgcgtc tggcgcggac cagcaacagg gatcggctgg
     6601 agccagcaga acctgcttag caccatgctc gacaaggtcc gacagcctta ttgcgacggc
     6661 aactcggcca tcagctcgat tgcggccgct cccatcacca gcggcgcttt ggccggaaac
     6721 ggtgcggagc agctttacgt cggtatggcc ggcgctttgg atgggggcgg actgattccc
     6781 ggccacgtgc tcaccgccgt cataaacaac gactcgcaat ccgccaccac cacctggatc
     6841 gatcgctatg catcccccgt cacgaaccag ggaatcaccg ccggacagtt caacccgcag
     6901 ggctacggca tctccagcat cgttcccgat ccgcacgacc cgaccggtca gacggtctac
     6961 gtcacgctcc gcggtatctc gggcacttct tcgttcatcc ccgtcgtcta ccgatccacc
     7021 gacgcgggcg cccactgggc gaacatcggc tccaatctgt cctcaactcc ggccaacagt
     7081 ttcctggtgg atcccaacga cgccaacatt gcctacatcg cgaccgatgc cggcgtcttc
     7141 ttcacccaaa agatctccag ctgcctctcc atgaacagtg cgtgctggaa cgtctacggc
     7201 agtgggcttc ccacctcacc cgctaccagc ctcatggcct acaaccaggg cgcgacccag
     7261 aagcttcgcg ctgccacgtg gggccgcggc atctgggaag cggatctcgc caccgcagga
     7321 atcgccccga ccaccgctac cgtcgagccc tcctcgctca ccttctccgg ccaacaggtc
     7381 cagaccacca gcgctccgca gaccatcaca atcaccaacg ccggcaatct caatctgaat
     7441 gtatccagtg tcgacatcac cggtgacttt actgaaaccg acaactgcat cgatcagtcg
     7501 ctcgaccccc acgcaagctg tcagattgca gttgtttttg ccccctccca gacgggaccc
     7561 gccagcggac tcctcaccct ttacgccaac ctcgccggcg ggcagctcac cgtgcctctg
     7621 tcgggcaccg gccttgcacc cgcatcgttg cgcctcacgc ccccgtcgct gacctttccc
     7681 ggcaccaccg tcgcctcccg ctccgatccc gagtacgtca cccttgccaa caccggcgac
     7741 atcgctgcgt ccctcgccgg cgagaccatc acaggggact actccatcgc cgccaacacc
     7801 tgcggcgcct ccatcgctcc gaacagcagc tgcaccctcg gcatcgtttt tactccaacg
     7861 gcctccggaa cccgaaccgg catcctcacc gtctcggatt cggtcggaat ccaaaccctg
     7921 cctctctccg gttccggcca gagcccgccg actgacacgc tctcgtccct gtcgctcaac
     7981 ttcgcaccgc agcaggtcgg caccacgagc acggcgcagc c
//
LOCUS       DASZYN010000340         2822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_782311, whole genome shotgun sequence.
ACCESSION   DASZYN010000340 DASZYN010000000
VERSION     DASZYN010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782311"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VHE33_08815"
     CDS             <1..237
                     /locus_tag="VHE33_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97597.1"
                     /translation="LSPTFRFGPVLVDPQRIRMPVPIDLNGTWSWDHRADINTWQEQE
                     VTNATQDALLPPDPASGTEGWLRLTPPPAPSNQQ"
     gene            248..1444
                     /locus_tag="VHE33_08820"
     CDS             248..1444
                     /locus_tag="VHE33_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HVT97598.1"
                     /translation="MGVVDFVEDVLTDLHLITPSTVDPFPSAYALTFDTSNASFNACQ
                     TSATAAAAVKDLCGAVVDLTGNPANPVYAGHNDNDMLYCGSMVKMFTMYVAFELKARV
                     QKQAKNMIAGGLSTATAGWENKVYAELRKAWKPKLDAAFPGLPSGMPDFKTIFQLSNT
                     GDATFPTQKTAADIDNIGEFGTPAGLYGDWMKLMLRWSNNTAAGQCIFPLSYSYLNGV
                     LGSAGFFDSATKKGLWVSGDYRKHDWVKGPGNKGGQALAPRFATAQGRTKSNFTGTAF
                     QVARLLTLMAQGKLIDATSSADMISSLTQGILDPSTGKSGTVSFIGDALAAASRPVSE
                     IRGKIGFGNDSFAHDCGIIRVDRGADPARTLRFIEVVLGAAPTNSAGFDQLAVAYYDC
                     VTAQHP"
     gene            complement(1497..2276)
                     /locus_tag="VHE33_08825"
     CDS             complement(1497..2276)
                     /locus_tag="VHE33_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HVT97599.1"
                     /translation="MRRRRHVHEEHTSHERWLVSYADFITLLFAFFVVLFASVYRDNK
                     NIVRLSNAIHNAFQNMGAFSSHAGAQNAYVIPAESMGPLQSDQQLELARQRAAAASQA
                     EVQQLRSELQAAMGDALKNHEMDMRVTPEGFVVSLRELGFFDSGQAELLPGAANKITR
                     IAKILTTHGLELRVEGHSDNQPINNSDFRNNWELSTARAMTVLLLMVDKAGYDPTKIS
                     VAGYAQYRPIANDSTPEGRRMNRRVDLVVVGTAPSAIAQKK"
     gene            complement(2273..>2822)
                     /locus_tag="VHE33_08830"
     CDS             complement(2273..>2822)
                     /locus_tag="VHE33_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HVT97600.1"
                     /translation="YAFKARREGLLSLDSELTKIEDPFLKESLMLAIDGVAAADLRKM
                     MELQIDYRGEKEERIPKVFESAAGYSPTIGIIGAVLGLIQVMQHLQDIDAVGRGIAVA
                     FVATIYGVAAANLVFLPCAGKLKIRIRERQVIQEMTLEAVLAILEGVNPRALELQLVS
                     YLPAKPKRSLSKVASTAKAAAQ"
BASE COUNT          559 a          866 c          803 g          594 t
ORIGIN      
        1 ctctcgccca ccttccgatt cggccccgtt ctggtcgatc cgcagcggat ccgtatgccg
       61 gtacccatcg acctgaacgg cacctggagc tgggaccatc gcgccgacat taatacgtgg
      121 caggaacagg aagtgaccaa cgccacccag gacgcgttgt tgcccccgga tcctgcgagc
      181 ggcaccgaag ggtggctgag gctcacgcca cccccggcac catcgaacca gcaatagagg
      241 aggtcgcatg ggcgtagtgg attttgtcga agacgttttg acggacttgc atctgatcac
      301 accgtcgacg gtggatccat ttccctcggc ttacgcactc accttcgata cttcgaatgc
      361 atcgttcaac gcgtgccaga ccagtgccac cgctgcggct gccgtgaaag atctttgcgg
      421 tgctgtggtg gatctcaccg gcaatccggc gaaccccgtc tatgccggcc acaatgacaa
      481 tgacatgctc tattgcggca gcatggtcaa gatgttcacg atgtatgtgg cattcgagct
      541 gaaggctcgc gtacaaaagc aagcaaaaaa catgattgcc ggtggtctgt cgaccgcgac
      601 cgcaggatgg gaaaacaagg tttatgccga gctcagaaaa gcctggaagc cgaaactcga
      661 tgcggcattt cccgggctcc ccagtgggat gcctgatttc aagaccatct tccaactttc
      721 caataccggc gatgcgacat tccccacaca gaagacggca gccgacattg ataacatcgg
      781 cgaattcggc actccggccg ggctgtatgg agactggatg aagctgatgt tgcgctggtc
      841 gaataacaca gccgcgggcc aatgcatttt cccactgagc tattcctacc ttaatggggt
      901 gctgggttca gcgggctttt tcgattcggc aacgaagaag ggcctatggg tctcgggaga
      961 ctatcgcaaa cacgactggg tgaagggtcc ggggaataaa ggcggacagg ccctggctcc
     1021 gcgcttcgcg acggcgcagg ggcggaccaa atcaaatttc accggcacgg cctttcaggt
     1081 cgctcgcctg ctgacgctga tggcgcaggg caaactgatc gatgcgacct cgagcgccga
     1141 catgatcagc agtctgaccc aggggatcct cgacccttct accggcaaga gcgggacagt
     1201 cagctttatc ggcgatgccc tcgccgcggc atcgcgcccg gtgagtgaga tccgcggaaa
     1261 aatcggcttt ggcaacgatt cctttgcgca cgactgcgga atcatccgcg tcgatcgggg
     1321 cgcggatcca gcccgaacgc ttcgcttcat cgaagtggtt ctcggtgctg cgccgaccaa
     1381 cagcgccggc ttcgatcagc tcgccgttgc ctactatgac tgcgtgactg cacagcatcc
     1441 ctgaatcaca acgggcgcgg ttcatcgggt ttgcgcgggt tcgttttgga gcgcagttac
     1501 ttcttctgcg cgatcgccga gggagcggtc ccgacgacca ccagatccac tcttcggttc
     1561 atccggcggc cttccggggt cgaatcgttc gcgatgggcc ggtattgcgc ataaccggcg
     1621 accgaaatct tcgtcggatc ataaccggct ttgtctacca tcagcagcag cactgtcatt
     1681 gcgcgtgcgg ttgaaagctc ccagttgttg cggaaatccg aattgttgat gggctgattg
     1741 tcggaatgcc cttccactcg cagttcgagc ccgtgcgtcg tcaggatttt cgcgatacgc
     1801 gtgatcttgt tggcggctcc ggggaggagt tctgcctgtc cactatcgaa gaaacccagc
     1861 tcgcgcaggc tcaccacaaa tccttcgggg gttacgcgca tgtccatctc atgattcttc
     1921 agcgcatcac ccatcgccgc ctgcagttcg ctgcgcagct gctgcacttc cgcctgcgat
     1981 gccgcagcgg cccgttgcct ggccagttcc agttgctggt cggattggag cgggcccatg
     2041 ctttccgctg gaatcacata ggcattctga gcgccggcgt gactggaaaa tgcgcccata
     2101 ttctggaacg cattatggat ggcgtttgaa agccgcacga tgttcttgtt gtcgcgatac
     2161 accgaggcaa acagcaccac gaaaaacgcg aagagcagcg tgatgaaatc ggcgtatgac
     2221 accagccagc gttcgtggct ggtgtgttct tcatggacgt gccggcgtct cctcattgcg
     2281 cggcggcctt cgccgtggac gctaccttcg acaagctgcg ctttggcttc gccgggagat
     2341 aactcaccag ctgcaattcc agcgcacgtg gattcacacc ttccagaatt gccagcaccg
     2401 cctccagcgt catttcctgg atgacctggc gctcgcgaat ccggatcttt agcttgcccg
     2461 cgcacgggag gaacaccagg ttcgccgctg ccactccgta gatcgtcgcc acaaacgcca
     2521 cggcgatgcc gcgtcctact gcgtcgatgt cctgcagatg ctgcatgacc tgaatcaacc
     2581 ccagcaccgc gccgataatg ccgatggtcg gcgagtaccc ggctgctgac tcaaacacct
     2641 tcgggatccg ctcctctttt tcgccgcgat agtcgatctg taactccatc atcttccgca
     2701 aatcggcggc tgccactccg tcgatggcga gcatcaggct ctctttgaga aacggatcct
     2761 caatctttgt cagctcgctg tccagcgaga gcagcccttc gcgccgcgct ttgaacgcgt
     2821 ag
//
LOCUS       DASZYN010000341         2807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_205511, whole genome shotgun sequence.
ACCESSION   DASZYN010000341 DASZYN010000000
VERSION     DASZYN010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205511"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1182)
                     /locus_tag="VHE33_08835"
     CDS             complement(214..1182)
                     /locus_tag="VHE33_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97601.1"
                     /translation="MHESLLMCVSPWVVLLLTGETGSAAKVFWLTIGLTAIPGILGGL
                     TYGVSVYLKAVDARDDKWPPSGRLSGSVYFMAQGASGLGGAIAALLVILWAKRFPDNL
                     SDGVSLLNLAALAFVAGFAANRLLPMIADSVSNQLSKLIQKTDETATKADAAKEAVQR
                     AEAKVKQATDLATVLVRASDYLRSKSFIPKQTADLLTTLETLNKEFPTNRTLNILLAR
                     MYDEAAGKRAKAVEILKGFIDGKVKAGEGKDTDVAAAWWNIANYHEFDFTTAKTPALR
                     ASALEALDHALAIDKHTYYEKLTTDDDFKDLVASPEGQKLLSAYKP"
     gene            1376..2008
                     /locus_tag="VHE33_08840"
     CDS             1376..2008
                     /locus_tag="VHE33_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97602.1"
                     /translation="MSQKKICLAGLALLLAAAGCHKNSVDPGAFKSALNGYYSAYHVC
                     VWSAPVKFPAQADTNNDEQTKGFDALTDAGLLVRTPHEKKRFLIGSKQVNDYDVSDKG
                     RSTWTADASQPGYGNFCFGHREVSTIDSFTPPDADATQYTVNYHDTVTNVPDWANSAE
                     MKTAFPKIAALQSGQQAATATLVKSSNGWQVTNVNPAPDTSNANSNGLVQ"
     gene            complement(2097..>2807)
                     /locus_tag="VHE33_08845"
     CDS             complement(2097..>2807)
                     /locus_tag="VHE33_08845"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HVT97603.1"
                     /translation="RIAPGDTVILSSRVIPGNEKPIFRVIDHLCRRDADVIFDDGSHG
                     LIHVSGHASQEEQRLMINLLKPKFFIPVHGDYRHLKKHAEVAQRLGVVDLAMVIENGE
                     ILELDRNNATKNGKVTAGRVCIDSGSSTDVVEDLVIRDRRHLSEDGFVLPILTINKLS
                     GKVERQPELVSRGFVGAEPGLMEGARQVVTATLDSSSPEEKADYGVIKEKIRIDLKRY
                     IQKNTSRRPLIMPVILEI"
BASE COUNT          550 a          931 c          753 g          573 t
ORIGIN      
        1 aatcgtggct tgtgctgcag catcaaaggg ttatggccgg tttcggtcca acgatgcagc
       61 tgccccggcc gtggtactga agaacgctcg ggctgcgtct aaagagtggt atattcctga
      121 tcgagagatt cctgtgagaa agatcgagac gacctaaccg aaaagcactt ctcaatggct
      181 ccatcgccct cccgcccccg gcggtcgagg gcgttatggt ttataggcac tcaacagctt
      241 ttgcccctcc ggactcgcca ccaggtcctt aaaatcgtcg tccgtcgtca gcttctcgta
      301 ataggtatgc ttatcgatcg cgagcgcatg atcgagcgcc tccaaagctg acgcgcgcag
      361 ggcgggcgtt ttcgccgtcg tgaaatcgaa ctcgtggtag ttggcgatgt tccaccatgc
      421 tgccgcgaca tccgtgtcct tcccctctcc ggccttcact tttccgtcaa tgaatccctt
      481 caggatctcg actgctttcg cccgcttccc ggccgcctcg tcatacatcc gtgccagcag
      541 aatgttgagt gtgcgatttg tggggaattc cttgttcagc gtctccagcg tggtcagcag
      601 gtccgctgtc tgtttcggga tgaacgattt gctgcgcaga taatcgctcg cccgcaccag
      661 cacggtcgca aggtcggtcg cctgtttcac cttggcttcc gctcgctgga cggcctcctt
      721 cgctgcatcg gccttggttg cagtctcgtc ggtcttttgg atcagtttgg agagttgatt
      781 cgatacgctg tcggcgatca tcggcaacag ccggttcgcc gcgaagcctg ccacaaaagc
      841 cagcgctgcc aggttgagga gagaaacacc gtcggagagg ttgtcgggga aacgctttgc
      901 ccagaggatc accagcagag ccgcaatcgc cccgccaagg ccactcgctc cctgcgccat
      961 gaagtagacg ctcccgctca gccgtccgga gggaggccac ttatcgtccc tggcatcgac
     1021 cgccttgaga tagaccgaaa cgccataggt caggccgccc aggattcccg ggatcgccgt
     1081 cagcccgatc gtcagccaga aaaccttcgc ggcagagcct gtttcgccgg tcaggaggag
     1141 aacaacccag ggtgagacac acatcaggag ggattcgtgc atgttgtccg ccagggagtc
     1201 agggttggat cagggcctca ctctactcct ttttcttctt acgagacagt ctctggggcg
     1261 ctggcattgg ctctttggcc gggcttccgc ctcaaaggtt gccccgcatt ctcctccatg
     1321 ctgtgccctc acccagccag cggcccgcat cctataagcc gaggagaaac aacgaatgtc
     1381 ccagaaaaaa atctgcctcg cgggccttgc cctgctcctg gctgctgccg gatgccacaa
     1441 gaactccgtg gatccaggcg cgttcaagtc cgcactgaac ggctactaca gtgcttacca
     1501 cgtctgtgtc tggtccgctc ctgtgaagtt tcccgcccag gccgacacca ataacgacga
     1561 gcaaaccaaa ggcttcgacg ccctcaccga cgccggcctt ctcgtgcgca cgccccatga
     1621 aaagaagcgt ttcctgatcg ggtccaagca ggtcaacgat tacgatgttt ccgacaaggg
     1681 ccgctcgacc tggaccgccg acgcatcgca gccaggctac ggcaacttct gcttcggcca
     1741 tcgtgaagtg agtacgatcg acagcttcac cccgcctgac gccgacgcca cgcagtacac
     1801 ggtcaactac cacgacacgg tgaccaacgt gcccgactgg gccaacagcg ccgagatgaa
     1861 aacagccttc ccgaagatcg ccgcgctcca gtctggacag caagccgcca ccgcaacgct
     1921 ggtcaaaagc tccaacggat ggcaggtcac caacgtgaat cctgcgcccg acaccagcaa
     1981 cgcgaacagc aacggcctcg tgcaataacg actcgaagcc cggaaccaaa gaaaacgccc
     2041 cggtccagga ccggggcgtt tgctttccaa cttcttcctt ccggcttccg gcttttttag
     2101 atctccagga tcaccggcat gatcagcggc cgccggctgg tgttcttctg gatgtaccgc
     2161 ttcaggtcga tgcggatttt ctccttaatc acgccataat cggccttctc ttccggactc
     2221 gacgaatcga gcgtcgccgt gaccacctgg cgcgcgcctt ccatcagccc aggctcggcg
     2281 ccaacaaacc cgcgcgaaac cagttccggc tgccgctcca cctttccgct caacttatta
     2341 atggtaagga tcggcagcac gaacccgtct tccgacaaat gccgccggtc gcggatcacc
     2401 aggtcttcca ccacatccgt cgaagatcct gagtcgatgc acacccgccc cgccgtgacc
     2461 ttgccattct tcgtcgcgtt gttgcggtcc agctccagga tctcgccgtt ctcgatcacc
     2521 atcgccagat ccaccacgcc cagccgctgc gccacctcgg catgcttctt caggtgccgg
     2581 tagtctccgt gcaccgggat gaaaaacttc ggcttcagca gattgatcat cagccgctgc
     2641 tcttcctggc tggcatgccc gctcacatgg atcagcccat gcgagccgtc gtcaaagatc
     2701 acgtcggcat cgcggcggca gaggtggtcg atcacgcgaa agatcggctt ctcgttgccg
     2761 ggaatcacgc gcgagctcag gatgaccgta tcgcccggcg caatgcg
//
LOCUS       DASZYN010000342         5585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_976887, whole genome shotgun sequence.
ACCESSION   DASZYN010000342 DASZYN010000000
VERSION     DASZYN010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976887"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VHE33_08850"
     CDS             <1..226
                     /locus_tag="VHE33_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HVT97604.1"
                     /translation="SAAFLDLLADTAIKETKKSGVFVIPGLGRLVKAERKARMGRNPQ
                     TGEQIKIKAKTVVKFRVAKSAKDSIAPPKK"
     gene            555..2063
                     /locus_tag="VHE33_08855"
     CDS             555..2063
                     /locus_tag="VHE33_08855"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HVT97605.1"
                     /translation="MNVLLIYPQFPDTYWSFRYALSFQGKRAAQPPLGLLTVAALLPR
                     SWNKKLIDTNVERLRDRDLAWADVAFIGGMHVQHRDLIAIARRCRERGLRTVVGGPIT
                     SSMPAGELECDHIVIGEAENLIDGLAEDLERGTARAVYQNAERPALESTPAPDLSLIR
                     MRHYSTMTVQYSRGCPFSCEFCDIIEIYGRRPRTKPVAQVLAELDQLYAAGWRGPVFI
                     VDDNFIGNKVQARVLLAAIREWQAAHGSAFRFITEASLNLADDPGLMQAMQDAGFASV
                     FLGIETPDESSLAATNKLQNTRRDLLESVSRIQAYGIEVMGGFILGFDTDREDVFDRL
                     VEFIEKSAIPVAMVGLLQAMPGTQLFRRLSREGRILDNGGGNNTDCQLNFRPRMHADR
                     LLAGYRSVLRRIYSSESYYQRVRLYLIRCRPRDRGDFSFSNLRALFLSVLRQGVFGRS
                     RTSYWKFLLAAATRYRRSFGAAMTMAVMGYHFQVITEQIVRGEVTSGTAPVL"
     gene            2188..2388
                     /locus_tag="VHE33_08860"
     CDS             2188..2388
                     /locus_tag="VHE33_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HVT97606.1"
                     /translation="MEQGTVKWFNDAKGFGFLSRPNGEDVFVHFSAIQSNGFRSLQEG
                     QAVQFNVVKGPKGWQAENVQPL"
     gene            2489..2896
                     /locus_tag="VHE33_08865"
     CDS             2489..2896
                     /locus_tag="VHE33_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97607.1"
                     /translation="MSKLPPGRYQLKTLHEEIDLFDRKLAHLLRYEEFATDAARNSAA
                     RNLTTKREALVRAAREMVSEGIEYNDAELPRSFRTDPAAVADTPAIEPPDEEPAETVA
                     HARRSSSHPSPYAGTSMDVQKGLQEYKRRKKKA"
     gene            2985..3869
                     /locus_tag="VHE33_08870"
     CDS             2985..3869
                     /locus_tag="VHE33_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT97608.1"
                     /translation="MALLSIENLSIRFGATGAVRRLSLDIAPGESLGLVGESGSGKSV
                     TALAILRLLDPAATVEGRIAFDGRDLLALDAEAMRRMRGREIAMIFQEPMTALNPVLP
                     VGEQIAEAVRVHQRDGSRQQLREKVLESMDAVALPDPSRRYGDYPHQFSGGQRQRILI
                     AMAIANRPRLLIADEPTTALDVTVQAQILELLADLRRRFGLAMLFISHDLAVVSQVAD
                     RVAVMRQGLLLEQASRAAIFTQPLHAYTRSLLGAVPTMRTDLTKPLAQLEPGSPGKGA
                     QTAELPLREVAPGHWVRI"
     gene            3947..4729
                     /locus_tag="VHE33_08875"
     CDS             3947..4729
                     /locus_tag="VHE33_08875"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HVT97609.1"
                     /translation="MRALLLSDIHANIEALEAVMAAAPAHDVVWNLGDVVGYGANPNE
                     VIEQTRRLGSVFVRGNHDRVCSGLGGIEEFNPVAQRAALWTECVLTGEHRDWLRHMAV
                     GPIAPDGPYVRCVHGSPVDEDDYVMTVRDAWPALQATDARITFFGHTHVQGGFATNGD
                     EWFRLTPGYEQHRSRHPSHTLDDQSDQFELELRDGARYLLNPGSVGQPRDGDWRAAFA
                     LYDDQRMVLTWYRVPYNLKEAQERIVQAGLPDRLATRLRDGR"
     gene            complement(4871..>5585)
                     /locus_tag="VHE33_08880"
     CDS             complement(4871..>5585)
                     /locus_tag="VHE33_08880"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVT97610.1"
                     /translation="VGAGTMTAPLLILFLHVPVPVAVGTALAYSAAVKLLVVPVQIAR
                     GQVEWRTLGLMLLSGVPGVVLGTVLFRYAVALKSDKVWLYFALGGMIAFSSAWHIYRH
                     FRPARAGQNRRERPRWLAAAMLPVGAEVGFSSSGAGALGTIALLGLTRLETTKIVGTD
                     LAFGLCLSLVGGGLHLAAGGFDAGLLAHLVIGGLLGAVAGSTLAPRIPARAMRLALSL
                     WLLAIGIQLCWQATTLAGS"
BASE COUNT         1052 a         1909 c         1590 g         1034 t
ORIGIN      
        1 gtcggccgca ttcctggatt tgctggcgga cacagcgatc aaagagacca agaagagcgg
       61 cgtttttgtc attcccggac tcggccgtct ggtgaaggca gagcggaaag cccgcatggg
      121 gcgcaatccc cagaccggcg aacagatcaa gatcaaggcc aagaccgtgg tcaaattccg
      181 cgtggcgaaa tcggcgaagg attccatcgc accgccgaag aagtagtttc gggttccgtt
      241 gttcgaaaag aggaagcggc tcgccgcttc ctctttcgtt ttcgcgcgct ctggtgtggc
      301 ctccccaagg tctgttgaca aagcgtcttg tcattccgag cgaaaggggc cccaaacgca
      361 tttcagtttg ggggtgttga gccgaggaac ctgctcttcg ccctgttcca tatgaatccg
      421 gggcctcaca ctggtttgta atcttttcgt attcccgttg caccgtgtcc cgccgggtgg
      481 acactgatac caggtgcggt gcatgcgcgg aattctgctg catggttcct gtgcggcccg
      541 tgccggaggg ccccttgaac gtcctgctga tctatcctca gtttcccgat acttactgga
      601 gttttcgtta cgcgctctcc tttcagggca agcgtgcggc acaacccccg ctggggctgc
      661 tcacggtggc tgcgctgctg ccccgttcgt ggaataagaa actcatcgac accaatgtcg
      721 agcgcctccg cgaccgcgat ctcgcctggg ccgacgtggc cttcatcggc ggcatgcatg
      781 tgcagcatcg cgatctgatc gcaatcgcgc gccgttgccg ggagcgtggg ctgcgtacgg
      841 tcgtcggcgg acccatcacc agcagcatgc cggccggcga gctcgaatgc gatcacatcg
      901 tcatcggtga agccgagaat ctcatcgacg gcctggccga ggacctcgag cgcggtacgg
      961 ctcgagccgt ctaccagaat gccgagcgcc cggcactgga gagcacgccg gcgcccgatc
     1021 tgagcctcat ccgcatgcgt cactacagca ccatgaccgt gcagtattcg cggggctgcc
     1081 ccttcagctg cgagttctgc gacatcatcg aaatctacgg caggcgtccg cgcaccaaac
     1141 ccgtcgccca ggttctggcc gaactggatc agctgtacgc cgccggctgg cgcggcccgg
     1201 tgttcatcgt cgacgacaac ttcatcggca acaaggtgca ggcgcgcgtg ctgctcgccg
     1261 cgatccgcga atggcaggcg gcccacggct ccgcgttccg ctttatcacc gaggcgtcgc
     1321 tcaatctggc cgacgatccc ggcctcatgc aggcgatgca ggatgcaggc ttcgcctccg
     1381 tctttctggg catcgagacg cccgacgagt ccagcctggc ggccacgaac aaactccaga
     1441 acacccgccg cgatctgctc gagagcgtca gcaggattca ggcctacggc atcgaagtca
     1501 tgggcggatt cattctcggt tttgataccg accgcgagga tgtcttcgat cgtctcgtgg
     1561 agttcattga gaagagcgct atcccggttg cgatggtggg gctgctccag gccatgccgg
     1621 gcacgcagct ctttcggcgt ctgtcgcgcg agggccgcat cctcgacaac ggcggcggca
     1681 acaataccga ttgtcagctc aactttcgtc cgcgcatgca tgccgaccgg ttgctcgccg
     1741 gctaccgttc cgtcctgcgc cggatttatt cgagcgagtc gtactaccag cgcgtccggc
     1801 tctatctcat ccggtgccgg ccccgcgacc gtggcgattt cagcttctcg aacctgcgcg
     1861 cactcttcct gtccgtcctt cgccagggcg tgttcggccg atcgaggact tcgtactgga
     1921 agtttctgct cgctgccgcg acccgttacc gcaggtcctt tggcgcggcc atgacgatgg
     1981 cggtgatggg ctatcacttt caggtcatca ccgagcagat tgtgcgcgga gaagtcacat
     2041 ccggtaccgc ccctgtgcta tagtggtggt taatcgtcga atccgaaatg gtgtttttgc
     2101 ctgttctgcc tgttcttgta actccgctga ctcgcaacaa tcccgcctca gagttcagcg
     2161 atacattcaa gtaaaaggaa acatgttatg gaacagggaa ctgtgaagtg gtttaacgat
     2221 gccaagggct ttggatttct gagccgtccc aatggcgaag atgttttcgt ccatttttcg
     2281 gcgattcaga gcaatggttt tcggtcgctg caggaaggcc aggcggtcca gttcaacgtg
     2341 gtcaaaggac ccaagggctg gcaggctgag aatgttcagc cgctctaagc agcagcagta
     2401 ttagaagaaa gggaggccgg acgtggcctc cttttgcttt tctggcagtc tctctgccca
     2461 acctcggctc gtcgccgatg gagtctctat gtccaagctt ccgcccggca ggtatcagct
     2521 caaaacactg catgaagaga tcgacttgtt cgatcgcaaa ctggcgcacc ttttgcggta
     2581 cgaggaattc gctaccgatg ccgcccgcaa ttccgccgcc cgcaatctca ccacgaaacg
     2641 cgaagccctc gtccgcgcag cccgcgaaat ggtcagcgaa ggcattgagt acaacgacgc
     2701 cgaattaccg cgatcgtttc gcaccgaccc ggccgccgtc gccgacacac ccgccatcga
     2761 gccgcctgac gaagagccgg ctgagactgt tgcgcacgct cgccgctcga gctcccatcc
     2821 ttctccctat gcgggaacct caatggatgt tcaaaagggt ctccaggaat acaaacggag
     2881 aaagaagaag gcctgaacaa acgcagagag aagaagacct gcttctctca gctcaggggc
     2941 gcgaaccgaa tcggcttgcg ccgcccgtcc tgtaggatgg cgctgtggcg ctgctctcca
     3001 tcgaaaatct cagcattcga ttcggcgcaa ccggcgctgt tcgccgtctt tcgctcgata
     3061 tcgctcccgg tgagtctctc ggcctggtcg gcgaatccgg atccggcaaa tccgtcaccg
     3121 cgctcgccat tctccgcctc ctcgatcccg cggccaccgt cgaagggcga atcgcattcg
     3181 atgggcgcga tctcctcgct ctcgatgcgg aagccatgcg ccggatgcgc ggccgtgaga
     3241 tcgccatgat cttccaggag cccatgaccg ccctcaatcc agtgctcccc gtgggtgagc
     3301 agattgccga agccgtccgc gtccatcagc gcgatgggtc gcgacaacag ctgcgggaaa
     3361 aggtgctcga atcgatggac gccgtcgcgc tgcccgatcc ctcccggcgt tacggcgact
     3421 atccgcacca gttctccggc ggccagcggc agcgcatcct catcgccatg gccatcgcca
     3481 accgtccgcg cctgctcatt gccgacgagc ccaccacggc cctcgacgtg accgtgcagg
     3541 cacagattct tgagctgctc gcggatttgc gccgccgctt tggactggcc atgctgttca
     3601 tctcccacga tctggccgtt gtctctcagg tcgcggatcg cgttgccgtc atgcgccagg
     3661 gtctcctgct cgagcaggcc tcgcgggccg caatcttcac ccagccgctg cacgcgtaca
     3721 cccgcagcct gctcggcgcg gtccccacca tgcgcaccga tctcacaaaa cctcttgccc
     3781 agctggaacc cggcagccca ggcaaaggtg cccaaaccgc cgaactcccc ctccgggaag
     3841 tcgctcccgg ccactgggtg cgcatttgac acatcgccgg cgctccggaa taaacgcgac
     3901 aaccgttagc gccatcgaat aaagtgataa acggccctgt ccgcctatgc gtgcgctgct
     3961 cctctccgat atccacgcca acatcgaggc tctcgaagcc gtgatggccg ctgctccggc
     4021 ccacgatgtc gtgtggaatc tcggcgatgt cgtcggctat ggcgcgaatc ccaacgaagt
     4081 catcgaacaa acccggcgtc tcggcagtgt ctttgtccgc ggcaatcacg accgcgtctg
     4141 cagtggtctg ggtggcattg aggagttcaa tcccgtcgcc cagcgtgcag cgctctggac
     4201 cgaatgcgtc ctcaccggcg agcaccgcga ctggctgcgg cacatggccg tcggtcccat
     4261 tgcccccgat ggcccgtatg tgcgctgcgt ccacggatcc cccgtcgatg aagacgacta
     4321 tgtcatgacc gttcgcgatg catggccggc cctgcaggcc accgacgccc gcatcacttt
     4381 cttcggccac acccacgtgc agggcggctt tgccaccaac ggcgatgaat ggttccggct
     4441 cacgcccgga tacgaacagc atcggtcccg ccaccccagc cacacactcg acgaccagtc
     4501 cgaccagttc gaactggagc tgcgcgacgg cgcgcgctat ctgctcaatc ctggctcggt
     4561 aggacagccc cgcgatggcg actggcgcgc cgccttcgct ctctacgacg accagcgcat
     4621 ggtcctcacc tggtaccggg tgccttacaa cctgaaagaa gcgcaggagc gcatcgtgca
     4681 ggcaggtctg cccgaccgcc tcgccactcg cctccgcgac ggccgctagt ccagtgtcgt
     4741 gtcccaaaac tttgctacgt aatgaaccgg ctgtcattcc gagcgaaagg ggccccaaac
     4801 gcctttcagt ttaggggtgg tgagccgagg aacctggttt tctcctcgta ggtgaaacag
     4861 gaacaacacc tcagctgccc gccagagtcg tcgcctgcca gcaaagctga atcccaatag
     4921 ccagcagcca cagcgaaagc gccagccgca tcgcacgcgc cggaatcctc ggcgcaagcg
     4981 tgctccccgc aacagcaccg agcaaacccc cgatcaccag gtgcgccagc aaaccggcat
     5041 cgaaacctcc cgccgccaga tgcagcccgc cccccaccag cgacaggcac aacccaaagg
     5101 cgagatccgt ccccacgatc ttcgtcgtct ccagccgcgt cagcccgagc agcgcaatcg
     5161 tgcccagcgc gcctgccccc gaagacgaga agccgacctc ggcgcccacc ggcagcatcg
     5221 ccgccgccag ccacctcggt cgctcgcgcc gattctgacc ggcccgcgcc ggccgaaaat
     5281 gccggtaaat atgccacgcc gacgagaagg cgatcatccc gcccagcgca aagtagagcc
     5341 agaccttgtc gctcttcaga gccaccgcgt agcgaaacag caccgtcccc agcaccactc
     5401 ccggaacccc gctgagcagc atcaacccca gcgtgcgcca ctctacctgc ccgcgcgcga
     5461 tctgcaccgg caccaccagc agcttcaccg ccgcggagta ggccagcgcc gtccccacag
     5521 caacaggcac aggcacgtgc aggaacagga tcagcagcgg cgcagtcatg gtccccgcac
     5581 caact
//
LOCUS       DASZYN010000343         7225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_333100, whole genome shotgun sequence.
ACCESSION   DASZYN010000343 DASZYN010000000
VERSION     DASZYN010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333100"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..1979
                     /gene="glgP"
                     /locus_tag="VHE33_08885"
     CDS             246..1979
                     /gene="glgP"
                     /locus_tag="VHE33_08885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HVT97611.1"
                     /translation="MVAYFSMEIAVAPEFPTYSGGLGVLAGDTLRAAADMGLPLAAVT
                     LVDRKGYFRQHLDASTGIQTEEAQPWSPESILTAEEPIVTVTLEGRPVAVRGWRYDLE
                     GVTGHRIPVYFLDTDLEQNDPKDRSLTDVLYGGDPDYRLRQEAILGIGGARLLRALEY
                     EPAVYHMNEGHAALLTVPVLEQELHGAGLQTATERDLEAVRRRCVFTTHTPVPAGHDR
                     FSGEQAYRILGAERAGMLERFGCFHDGLLNMTYVALRFSRFVNGVAMQHGKVSRQMFP
                     EYTIDAITNGVHAGTWTAPPVQAVFDKHLPRWRQDNVTLRYAIDIPENEIVEAHAASK
                     QVLIDRVKAASGVEMNPDVFTLGFARRAATYKRADLLFTDPERLVKIAEKFGGLQIVY
                     AGKAHPADEPGKAKIRRVIELARDLRGGPLQVVYLENYEWRLGAVLTGGVDLWLNTPR
                     RPYEASGTSGMKAALNGVPSLSILDGWWIEGWIEGVTGWAIDDHDDEAVEAKSLYDHL
                     EKLILPLYTKDTGQWRRIMRSTIALNGSFFNTQRMLEQYVINAYFPERPVDRMPESKS
                     PQPDLENSVAQ"
     gene            2172..3635
                     /locus_tag="VHE33_08890"
     CDS             2172..3635
                     /locus_tag="VHE33_08890"
                     /inference="COORDINATES: protein motif:HMM:NF006088.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HVT97612.1"
                     /translation="MNQPSVEALAPEVSRRPLCVDLDGTLAKNDTLMDSLLVLVRNRP
                     LDALRVPLWLLRGKAAVKAEIGARVALDVQHLPYNRSVVAYLEVERGEGRRLYLATGA
                     DELLAKRVADHLALFDGVLASDGRTNLTAANKLESLRRRFGNDGYDYIGNAGPDLPLL
                     EHAGTAMIANPARGLRSRLQSRGITVRREFEDRRAPMKAILKALRLHQWAKNILIFVP
                     MLLAHVLRAGVFADAAIAFVSFSLCASATYVANDLLDIESDRRHPRKRNRPFAAGDLS
                     VLTGVLISGICLGGGIAIAAAVLPRGFLLWLFLYIVGTLSYSLALKRAMLVDVMVLAG
                     LYTVRLLAGAAATRVEISPWFAAFAIFLFLSLAMVKRFSELENTRARGEAIANGRGYR
                     LGDIEQLRSFGTSNATAAVLVFMIYIGISRDVNSLYRHPNWLWLIAPLMIYWLSRVWL
                     LASRGELDEDPVIFAVTDRISLMTGAAVAVVAALAAF"
     gene            3659..5005
                     /locus_tag="VHE33_08895"
     CDS             3659..5005
                     /locus_tag="VHE33_08895"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HVT97613.1"
                     /translation="MGESGKPTEQAPSFESWGRYPKLQAEVVPLHWPSDFPLPQAPAT
                     RQLPVGLGRSYGDVCLLENGTLLQTSGMDRLLYFDPTNGLLRCEAGVSLQQILDFAVP
                     RGFFLPVTPGTKFVTVGGAIANDIHGKNHHVAGTFGRHVQRFELVRSDGNRFVCSPVE
                     NPEWFAATIGGMGLTGLITWVEMHLRPIVSRKIEYRGEKFHGLDEFFALSQTATSEYT
                     VAWIDCVSTGSNFARGIFMQGDHSETPGPLTKSKEPKLTFPFNLPEFALNRFSMAAFN
                     TLYFHKQIGKQKAGPVDYEPFFYPLDAVQHWNRMYGRQGLLQFQNVLPHESGREGMIQ
                     ILKAITKSGLASFLAVIKFFGDVPSVGMMSFPKPGPMLALDFPIRREVSFDLVDRLAR
                     ITLEHGGRMYSAKDARMTPEQYQAFYPQWQDFSRFVDPGFDSAFWQRVTGRRPLVG"
     gene            4998..5762
                     /locus_tag="VHE33_08900"
     CDS             4998..5762
                     /locus_tag="VHE33_08900"
                     /inference="COORDINATES: protein motif:HMM:NF005489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT97614.1"
                     /translation="MAERQATATPKRILALGATSAIAQAALRLLAERGAVFFVVGRNA
                     QKLAAVRDDLLTRGAGAVTAYVADLDDTPAHPAMLAHAVSVLGGLDVALLAHGVLGEQ
                     PVAQMDYTTAEAILRTNFLSAVSLVTWLANYFEAERRGTIAVVSSVAGDRGRKSNYVY
                     GASKGGLNIFLDGVRNRIDRAGVKVLTIKPGFVATPMTAHLRKNALFAEPGRVGKDIV
                     RAIDKRKDVAYTPPFWGMIMLVVRSVPGRIFKRLNL"
     gene            6038..>7225
                     /locus_tag="VHE33_08905"
     CDS             6038..>7225
                     /locus_tag="VHE33_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVT97615.1"
                     /translation="MWIVRLALRRPYTFAVLALLLMVLGPLAIMNMPVDIFPNIDIPV
                     VSIIWQYQGFSPEQMADRIVALSERSLTTTVNDIEHIESMSVNSRSVIKVFFQPGVNI
                     GNAVAQITSIAQTQLRQLPPGTTAPLVIQYNASSVPIVQLGLSGQGLSEMQLNDYALN
                     FLRTQLVTVPGAGLPYPYGGKQRQVQVDLNLPALQSKGLSPADVVNAIGAQNLILPGG
                     TIKIGQFEYQLETNSAPATIQALNDLPIRQVNGAMVYIHDVAHVRNGFPPQTNIVRVD
                     GQRAALLSVIKTGNTSTLNIVKGILTKVDSLREQLPAQLRQLRIIPLGDQSIFVRNSI
                     DGVVHEAIIAACLTAIMILIFLGSWRSTLIIAVSIPLSILTSLLVLSALGESINIMTL
                     GGLA"
BASE COUNT         1342 a         1911 c         2485 g         1487 t
ORIGIN      
        1 tccggggacg agcgttcctg aggactgcga attttgaagg aatggaaagg attccacaga
       61 agggtcatac cgcttgccgg aacggcaatg taagaatcgc aggaagccga atctgagcta
      121 ggtggctgac gcagagattg ctattctgaa gccggacgtt ctaaagaggc cgacgaaacg
      181 ttttagcgat gcgctttctt aaccgtacgc aatacctatc ccccgatgaa ccaggagagg
      241 tgggcatggt cgcctacttc tcgatggaga ttgccgtagc ccctgagttt ccgacctaca
      301 gcggaggcct gggtgtgctg gcaggcgata cgctgcgagc cgcggccgac atggggctgc
      361 cgctggcggc agtcacgctg gtcgatcgca agggctactt ccgccagcat ctggacgcga
      421 gcaccggcat ccagacggaa gaggcgcagc cgtggtcgcc ggagagtatt ctcacggctg
      481 aagagccgat tgtgacggtg acactggaag ggcgtccggt ggcggtgcgc ggctggcggt
      541 acgacctcga gggtgtgacc ggacatcgga tcccggtgta tttcctcgac acggatctgg
      601 agcagaacga cccgaaggac cgcagtctga cggatgttct ttatgggggg gacccggatt
      661 atcggcttcg ccaggaagcg attctgggga ttggcggtgc gcggctgctg cgggcgctgg
      721 agtacgagcc ggccgtttat cacatgaacg aggggcacgc cgcattgttg accgttcctg
      781 tgctggagca ggagcttcat ggcgcagggc tgcagacggc gacagagcgt gatctggagg
      841 cggtacggcg gcgctgtgtg tttacgacgc atacgccggt gccggcgggg cacgaccgtt
      901 tctcgggcga gcaggcttat cgcatcctgg gtgcggagcg ggcgggaatg ctggaacgat
      961 tcgggtgttt ccacgacggg ctgctgaata tgacgtacgt ggccctgcgc ttttcccgct
     1021 tcgtgaacgg cgtggccatg cagcatggca aggtttcgcg gcagatgttc cccgagtaca
     1081 cgatcgatgc gatcacgaac ggcgtgcatg ccgggacgtg gactgcgcct ccggtgcagg
     1141 cggttttcga caagcacctg ccacgctggc ggcaggacaa cgtgacactg cgttacgcga
     1201 tcgacattcc ggaaaacgaa attgtggaag cgcacgctgc gtcgaagcag gttctgatcg
     1261 accgggtgaa ggcggcctcg ggtgtggaga tgaatccgga tgtcttcacg ctgggatttg
     1321 cgcggcgcgc tgcgacttac aagagagccg atttgctttt caccgatccg gagcggctgg
     1381 tgaagatcgc ggaaaagttt gggggcctgc agattgttta tgccggcaag gcgcatccgg
     1441 cggatgagcc gggcaaggcg aagattcgcc gcgtgattga actggcgcgc gatttgcggg
     1501 gaggcccact gcaggtcgta tatctggaga attacgagtg gaggctggga gcggtcctga
     1561 ctggcggtgt tgacctgtgg ctgaatacgc cgcggcggcc ctatgaagct tcgggaacca
     1621 gcgggatgaa agctgcgctc aacggcgtgc cgagcctgag cattctcgat ggctggtgga
     1681 tcgagggctg gatcgagggt gtgaccggct gggcgatcga cgatcacgac gacgaggccg
     1741 tcgaggcgaa gtcgctctac gatcacctgg agaagctgat cctgccgctg tacacgaaag
     1801 acacaggaca gtggcggcgg atcatgcgct cgacgatcgc gctgaacgga tccttcttca
     1861 acacgcagcg gatgctggag cagtatgtga tcaacgcata cttcccggaa cggccggtgg
     1921 acaggatgcc ggagtcgaaa tcgccgcagc cggacctgga aaactctgtc gcacagtagg
     1981 gccgcggacg ctcgcgacgt ggctgaaagc ctgtatcgac gctgattctc tgcaggggcg
     2041 ggctattggc gcgatgctat acttggcctg attcttagct ctcgtccgat ggagtgtgac
     2101 gagctgcaca ccgtcctcgt catggcgcag ttcgctcggg ttcttgtccg gaaaccatgg
     2161 gagagacttc cttgaaccag ccatccgtgg aggcgttggc acctgaggtg tcgcgacggc
     2221 cgctgtgtgt cgatctggac gggactctgg cgaagaacga cacgctgatg gactcgctgc
     2281 tggtgctggt gcgcaaccgg ccgctggatg cgctgcgggt tcctttgtgg ctgttgcgcg
     2341 gcaaggctgc agtcaaagcg gaaatagggg cgcgcgtggc actcgatgtg cagcatctgc
     2401 cgtacaaccg gtcggtcgtc gcctatctgg aagtcgagcg cggcgaaggg cgcaggcttt
     2461 atctggcaac gggggccgat gaacttctgg cgaaacgggt cgccgaccat ctggcgctgt
     2521 tcgacggcgt gctggccagc gacggcagga cgaatctgac ggcggcgaac aagctggaga
     2581 gtctgcgcag aaggtttggc aacgacggat acgattacat cggcaatgcg gggcccgatc
     2641 tgccgctgct ggagcatgcg ggcacggcga tgattgcgaa tccggcgcgg ggtttgcgtt
     2701 cgcgcctgca atcgcgcgga atcacggtgc ggcgcgaatt cgaggacagg agagcgccaa
     2761 tgaaggccat actgaaggct ctccgtctgc atcagtgggc gaagaacatt ctgatctttg
     2821 tgccgatgct gctggcgcac gtcctgcgcg ccggagtttt tgcggatgcg gcgattgcct
     2881 ttgtgagctt cagtctgtgt gcgtctgcca cttatgtcgc caacgatctg ctggacatcg
     2941 aatcggaccg gcggcatccg cgcaaaagaa atcggccctt tgcggcgggc gatctctcgg
     3001 tcctgaccgg ggtcctgatc agcgggattt gtcttggtgg aggcatcgcg attgcggctg
     3061 cggtgctgcc tcggggattc ctgctgtggc tgtttctcta cattgtggga acgctgtcgt
     3121 attcgctggc gctgaagcgg gcgatgctgg tggacgtgat ggtgctggcg ggcctctaca
     3181 ctgtgaggct gctggcaggc gctgcggcga cgagggtgga gatatcgccc tggtttgccg
     3241 cctttgcgat ttttctgttc ctgagtctcg ccatggtgaa gcgcttcagc gaactggaga
     3301 acacgcgggc acgcggggaa gcgattgcga acggacgcgg ctatcggctg ggtgacatcg
     3361 agcaactcag gagcttcggg acgtcgaatg caacggcggc ggtgctggtg tttatgattt
     3421 acatcggaat ctcgcgcgac gtgaacagtc tctaccgtca tcccaactgg ctgtggctga
     3481 tcgcgccact gatgatctat tggctgagcc gggtatggct gctggcttcg cggggcgaac
     3541 tcgatgagga tcccgtgatt tttgccgtta cggatcgcat cagtcttatg accggcgcgg
     3601 cggtggcggt ggttgcggct cttgccgctt tttaatgcag tgaaggtgaa cagtatccat
     3661 gggtgaatcc ggcaagccga cagaacaagc gccatcgttt gaatcgtggg gacggtatcc
     3721 aaaactgcag gcggaggtcg ttccgctgca ttggccgagc gattttccgc tgccgcaggc
     3781 gccagcgacc cggcaactgc cagtggggct ggggcgcagt tacggcgatg tgtgcctgct
     3841 ggagaacggt acgctgctgc agacatcggg catggaccgg ctgctctact tcgatcccac
     3901 gaacggcttg ttgcgctgcg aggccggggt ttcgctgcag cagattctgg atttcgcagt
     3961 gccgcgcgga ttctttctgc cggtgacgcc aggaacgaaa tttgtcaccg tgggcggagc
     4021 gattgccaac gacattcacg ggaagaatca ccacgtggcg gggacgtttg ggcgtcacgt
     4081 gcagcggttc gaactggtgc ggtccgatgg aaaccggttt gtgtgcagcc cggttgagaa
     4141 tccggagtgg tttgcagcga cgatcggggg catgggactg acggggctga tcacgtgggt
     4201 ggaaatgcac ctgcggccca ttgtgtcgcg caaaatcgaa taccgggggg agaagtttca
     4261 cgggctcgac gagttctttg cgttgtccca gaccgcgacc agcgaataca cggttgcatg
     4321 gatcgactgc gtttccacgg gcagcaattt tgcgcgcggg attttcatgc agggcgatca
     4381 ctcggagacg ccgggtccgc tgacaaaaag caaagagcca aagctgacgt ttccgttcaa
     4441 tctgccggag tttgcgctga accgtttttc gatggcagcc ttcaacacgc tctacttcca
     4501 caagcagatt ggaaaacaaa aggccgggcc ggtggattac gagccctttt tctatccgct
     4561 ggatgccgtc cagcactgga acaggatgta cggcaggcaa gggctgctgc agtttcagaa
     4621 tgtgctgccg cacgagagcg ggcgcgaggg catgattcag atcctgaagg ccattacgaa
     4681 atccggtctc gcgtccttcc tggcagtgat taagtttttt ggcgacgtgc cgtccgtggg
     4741 gatgatgtcc tttccgaagc cgggaccgat gctggcgctg gattttccga ttcggcgcga
     4801 ggtgagcttc gatctggtcg atcggctggc gcggatcacg ctggaacacg gagggcggat
     4861 gtactccgcc aaagatgcgc ggatgacgcc ggagcagtat caggcgtttt atccgcagtg
     4921 gcaggatttc tcccggttcg tggatccggg gttcgactcg gcgttctggc agcgcgtgac
     4981 gggacggagg ccgctcgttg gctgaacggc aggcgactgc aacgcctaaa aggattttgg
     5041 cgctgggggc gacttcagcg atcgcgcagg cggcgttgcg gctgctggcg gagcgggggg
     5101 cggtcttttt cgtggttggg cgcaatgcgc agaaactggc cgcggttcgg gacgatttgc
     5161 tgacacgggg agcgggcgcg gtcacggcgt atgtcgccga tctggacgac acgccggcgc
     5221 atccggcgat gctggcgcac gcggtttcgg ttctgggcgg cctggacgta gcgctgctgg
     5281 cgcatggagt gctgggcgaa cagccggtgg cgcagatgga ctacacgacg gcggaagcga
     5341 ttcttcgaac caattttctg tccgcggttt cgctggtaac gtggctggcg aattattttg
     5401 aagcggagcg acggggcacg attgcagtgg tttcttcagt cgccggggac cggggacgca
     5461 agagcaatta tgtgtatggt gcatcgaagg gcggactgaa catcttcctc gacggcgtgc
     5521 gcaatcgcat cgaccgggcg ggtgtgaagg tgctgaccat caagcctgga tttgtggcaa
     5581 cgccgatgac ggcgcacctg aggaagaacg cgctatttgc ggagcccggg cgcgtgggga
     5641 aggacattgt gcgcgccatc gacaagcgga aggatgtggc ctatacgccg ccgttctggg
     5701 gcatgatcat gctggtggtg cgcagcgtgc cggggcgaat ttttaagcgc ctcaacctgt
     5761 agagaaggaa cgttccatag gcggaaactg catcaggacg caccgtgcgg gcatctataa
     5821 ggggtcgctt tgaggccgga tccgaggtct ttttcggcgg cccattcgtc tccaacgtag
     5881 agcgattttc acgttcgaaa ttctttccct acaggtaaag gatggatact gttacatgcg
     5941 ggcattgtgc tcccgcattg cgcaaaccga cttgcctctg gaatcccgca ccccggcgtg
     6001 gagcgttccg ggaagccgca ggagacgctg aaccgatatg tggatagtcc gcctcgcgct
     6061 gcgacggccg tatacgtttg ccgtgctcgc gctcctgctg atggtgctcg gcccgctggc
     6121 catcatgaac atgccggtgg atattttccc gaacatcgac atccctgtgg tgtcgatcat
     6181 ctggcaatac cagggattct cgccggagca gatggccgat cgcattgtgg cgctgagcga
     6241 gcgttcgctg accacgacgg taaacgacat cgagcacatc gagtcgatgt cggtgaacag
     6301 ccggtcggtc atcaaggttt tctttcagcc gggagtgaat atcggcaacg ccgtggcgca
     6361 gatcacgtcg atcgcgcaaa cgcagctccg gcaattgccg ccgggaacga cggcgccact
     6421 ggtcatccag tacaacgcgt cgagtgttcc cattgttcag ctggggctct ccgggcaggg
     6481 gctttcggag atgcagctga acgactacgc gctgaacttt ctgcgtactc agctggtgac
     6541 ggtgccaggt gcaggacttc cctatccgta tgggggaaag cagcggcagg tgcaggtgga
     6601 tctgaatctg cctgcgctgc agtcgaaggg gctttcgccg gccgatgtgg tgaatgcgat
     6661 tggcgcccag aatctgatcc tgcccggggg cacgatcaag atcggccagt tcgaatatca
     6721 actggagacg aacagcgcgc cggctacgat tcaagccctg aacgatttgc cgatccggca
     6781 ggtcaacggc gcgatggtgt acatccacga tgtcgcgcac gtgcgcaacg gctttccgcc
     6841 gcagaccaac attgtgcgcg tggacgggca acgggcggca ctgctttccg tcatcaagac
     6901 cgggaatacc tccacgctga acatcgtgaa gggcattctg accaaggtgg acagcctgag
     6961 ggagcagctg ccggcgcagt tgagacagtt gaggatcatt ccgctgggtg atcagtcgat
     7021 ctttgtacgg aattcgatcg atggggtggt gcacgaagcg atcattgctg cgtgtctgac
     7081 ggcaatcatg attctgatct tcctgggcag ctggcggagc acgctgatta tcgcggtttc
     7141 gatcccgttg tcgattttga cgtcgctgct ggtgctcagc gcgctgggtg agtcgatcaa
     7201 catcatgacg ctcggcggcc tggct
//
LOCUS       DASZYN010000344         5380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_205613, whole genome shotgun sequence.
ACCESSION   DASZYN010000344 DASZYN010000000
VERSION     DASZYN010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205613"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4379)
                     /locus_tag="VHE33_08910"
     CDS             complement(<1..4379)
                     /locus_tag="VHE33_08910"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HVT97616.1"
                     /translation="MNAAPGCSNASATRSRFTGSQPRCRALLVASALILLCAWATSLH
                     AQTAAFNGGITTTINASFKGPMGVTTDPNGNIFVADNNTSTNTATVYEMTRNGPGSYG
                     SPVALPSPAGGFVCPSPVTEPDPCLRGLGLDSRGDLWVAAFGNFSTTAGHVYEWVNTS
                     GTFATPVAIGTGWKGPWGIAGDPSGNVFVSDNIANTISKISFSSGQPVVTTVVAANVV
                     AQPRGIAVDASDNIFVIDGNLDHLEKLQSPYTSESSLNNYSFQGPGDLARDAQGNLWL
                     SEFGTNLIREATSTLSNTVAGWGRGFSGPVSVWPAPDGTILVSDWTNHAIKQIVPQTD
                     NVGTVAVGSSPVTQTLQFTFTSNTPTTIKAPKVVTQGASGLDFTDLGTGTCNTNGTSR
                     SYDASDSCTVNVSFKPKSAGIRHGAAQLISNAGTVLATAPLYATGVGPQLAFAGGTFP
                     GTFLTQSIVNPQGLAVDGTGNIYFSSGSSVTEIPPGCATSACRVTMGGGFSQPRAIAV
                     DGAGNLYVADSGSNAVKLIPPGCASSSCVTTVSGFNQPRGVAVDNGGTIYVADTANNA
                     IKKVVAGCSFSTCVSTLASGFNQPQGVAVDGNGNVFVADTSNSAIKEIPAGCTSSSCV
                     ITLGGGFSTPVAVAVDGVGDIYTADNHTASVMAPGCGSSSCVTTVRQNSAEGYTGIAV
                     DGSGSVYLSDSLGSGIYTLQLASPPSLTFANTHAGSQSSDSPQTVTLRNIGNAPLTFP
                     VPGTGTNPSISANFKLDSSTTCPDVLTSSPAGTLAAGTSCGLAIDFLPTAIGSVSGST
                     VLTDNNLNASPSTTQSIALSGTGTSAVPTPTVTVAPSPSSITTTQSLAVTVTLSGGSG
                     NPVPTGTVSLSGPGYSGSPLTLSSGSATFNIPANTLSVGTDTLTASYSGDTNYTTASG
                     SNSVTVTKATPTVTASANPTTITTLQSTVVTVSVAGTPQATGTITLTSAGVTVGTGSL
                     ASGAVQITVAASALAVGADTLTATYSGDGVYTTATGSTSVTVNKATPTVTASPNPATI
                     TTLQSTVVTVSVAGTPQPTGTITLTSGGVTVGTGSLAAGSAQITVAASALAVGTDSLT
                     ATYSGDGTYATATGTTSVTVNKATPTVTASPNPATITTLQSTVVTVSVAGTPQPTGTI
                     TLTNGGVTVGSGSLAAGSAQITVPASALAVGANTLTATYSGDGTYTTATGTTSVTVNK
                     ATPTVTASPNPATITTLQSTVVTVSVAGTTQPTGTITLTSAGVTVGNGSLVSGTAQIT
                     VPASALALGTNSLTASYSGDSNYDIATGSTSVTVTKATPTVSVTPAPATITTLQSTVV
                     TVTVAGTTQPTGTITLTSGGVSVGSGALVSGTAQVNISASALTLGSNTLTASYSGDSN
                     YDIAAGSGVVTIIKATPTVSASANPATITNSQSTVVTVSVDGSVQATGTITLTSGGVT
                     VGSGPLNSGAAQITVPGSALALGT"
     gene            complement(4612..>5380)
                     /locus_tag="VHE33_08915"
     CDS             complement(4612..>5380)
                     /locus_tag="VHE33_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97617.1"
                     /translation="ETANSIAKITGSTATWINTTSVHQPGGMVFDSGGNLLILDGNVA
                     RVVQLTASSSYTSANNIGSANFGGPGNLTVDNLGNIWVTNYNANLIQEMTIASNFATV
                     LNFGTGLQTPVEIWQTFEGNILVSDEDHGVIRQIAPVVNVGTVAIGSTSAKQTLTFTF
                     TGSSTTTIQAPVVVTQGATGLDFADAGTGSCTTTNGAGHPYASKATCTVDVTVKPKFA
                     GTRYGAVKLLNTSGTVLATAYLYGTGVGPQLVFSGGF"
BASE COUNT         1009 a         1469 c         1800 g         1102 t
ORIGIN      
        1 gtacccaaag ccagcgcgct gccaggcacg gtgatttgtg ccgccccgct gttcagcggc
       61 ccgctgccta cggtgacgcc gccactggtc aacgtgatcg tgccggtcgc ctggaccgaa
      121 ccatcgaccg aaaccgtgac gacggtggac tgagagttgg taatggtagc cgggttcgcc
      181 gatgccgaaa cagtaggagt cgccttaatg atcgtaacca caccagatcc ggcagcgatg
      241 tcgtaattgc tgtccccgga gtagctggcc gtgagcgtgt tcgaacccag cgtgagagcg
      301 ctggcagaga tgttgacctg tgccgtcccg ctcaccagtg caccgctacc cacagagacg
      361 cctccgctgg tgagcgtgat cgttccagtc ggctgtgttg tgccggccac ggtgactgtc
      421 accactgtgg attgcaaggt cgtgatggtc gcgggggcag gcgtaactga tacagtgggc
      481 gtcgccttcg tcaccgtaac cgaggtcgat ccggtggcaa tgtcgtagtt gctgtctccc
      541 gagtagctgg ccgtaaggga gtttgtacct aacgcgagtg cgcttgccgg tacggtaatt
      601 tgggccgtcc cgctgacgag cgaaccgttg cctacggtga ctcctgcgct ggtgagcgtg
      661 atggtcccgg tcggctgtgt tgtgccggcg actgagaccg tcacgacggt ggactgcagg
      721 gttgtgatcg ttgccggatt cggcgatgcc gtcacagtgg gcgtcgcctt gttgaccgtg
      781 actgacgtcg ttccggtggc cgttgtgtag gtaccatcgc ccgaataggt ggcggttagc
      841 gtattcgcgc ccactgcaag agcgctggcg ggtacggtga tctgggctga tccggcggcg
      901 agtgaaccgc taccgacggt gactccgccg ttggtcagcg tgatggttcc ggtgggttgc
      961 ggcgtgccgg caactgaaac cgtcacgacg gtggactgca gagtcgtgat cgttgctgga
     1021 ttcggcgatg ccgtcacggt gggcgtcgcc ttgttaaccg tgactgaagt cgttccggtt
     1081 gcggttgcgt aggtgccatc gcctgaatag gtagcggtca ggctatcggt gcccacagca
     1141 agggcactgg cggcgacggt gatctgggct gatccggctg caagcgaacc ggttcctacc
     1201 gtgactcccc cgctggtcag cgtgatggtt ccggtgggtt gcggcgtgcc ggcgactgaa
     1261 accgtcacca cggtggactg cagagtcgtg atcgttgctg gattgggcga tgccgtcacg
     1321 gtgggcgtcg ccttattgac cgtcaccgaa gtggatcccg tcgccgtggt gtagacacca
     1381 tcccctgaat acgtagccgt tagcgtatcg gcacccaccg ccagggcgct ggcggcaacg
     1441 gtgatttgta cggctccgct tgcaagcgat ccggtgccaa cggtgacgcc tgcgctggtc
     1501 agtgtgatcg tgccagtagc ctgcggcgtg cctgcgaccg aaactgtcac cacggtggac
     1561 tgcagagtcg tgatggtggt tgggttcgcc gatgcggtca cagtgggtgt agccttcgtg
     1621 accgtaacgg aattagatcc actcgccgtg gtgtagttgg tatctccgga gtagctggcc
     1681 gtgagagtat cggtgcccac ggagagtgtg ttcgcgggga tattgaaggt cgccgaaccg
     1741 ctgctgagcg taagagggga gcccgaatag cccggtccac tgaggctgac tgtcccggtg
     1801 ggaacggggt taccgctgcc gccactcagc gtgacggtca ctgccagact ttgggttgtc
     1861 gtgatgcttg aaggagacgg cgccacggtg acggtcggtg taggcaccgc ggacgttccg
     1921 gtgccgctca gcgcgatgct ctgcgtcgtt gacgggctgg cgttgaggtt gttgtccgtt
     1981 agaacggttg agccggacac cgagccgatg gccgtgggaa ggaagtcgat ggccagtccg
     2041 caactggtcc cggcggccag agtgccggct ggagaggacg ttagaacatc cgggcaggtc
     2101 gtcgacgagt ccagcttaaa gttggcagag atactgggat ttgttcctgt gcccggaact
     2161 gggaaggtca gcggtgcatt gccgatattg cgcagggtga cggtctgagg actatcgctg
     2221 ctttgagaac cggcgtgggt gttggcaaag gtgaggctgg gcggagaggc caactgcaga
     2281 gtgtaaattc ctgagccgag cgaatcgctg agatagacac tgccgctgcc gtcgacagca
     2341 atgccggtgt aaccttcggc ggagttttga cgcacggtgg tgacacagct ggaggagccg
     2401 cagcccgggg ccatgaccga agccgtgtgg ttatcggcgg tgtagatatc gcccactcca
     2461 tccacggcga ctgcgacggg agtgctgaaa ccgccaccca gcgtgattac gcagctggac
     2521 gaggtgcagc ccgcggggat ttccttgatc gcgctgttac tggtgtcggc aacaaagaca
     2581 ttgccgttcc catccaccgc gacgccctga ggctggttga aaccgctggc cagggtggac
     2641 acgcaggtgg aaaaggaaca gcccgcgacc accttcttaa tggcgttatt cgcggtgtcg
     2701 gcgacgtaga tggtgcctcc attgtcgacc gccacgccgc gaggctggtt gaagccacta
     2761 acagtcgtta cacaactgga ggaagcgcag ccgggaggga tcagcttcac ggcgttgctg
     2821 ccggaatcgg cgacgtacag gtttccggcg ccgtcaacgg cgatcgccct gggctgagag
     2881 aaaccgccgc ccatcgtcac gcgacaggca cttgtcgcac accctggagg tatctccgtc
     2941 acactcgaac cggaggagaa atagatgttc ccggttccat cgacagctaa accctgggga
     3001 ttgacgatcg attgcgtcag gaacgtgccc ggaaaagtcc caccggcgaa cgccagctgc
     3061 ggacctaccc ccgtggcata gagaggagcg gtcgcgagca cggttccggc gttactgatc
     3121 aactgtgctg caccatggcg tatgccggct gatttcggct tgaaactcac gtttaccgtg
     3181 cagctgtcac tcgcgtcgta ggatcggctg gttccattgg tgttgcaggt cccggttccg
     3241 aggtcggtaa aatcgagccc gctcgccccc tgggtaacaa ccttgggcgc cttgatggtg
     3301 gtaggggtgt tgctggtaaa ggtgaactgc aaagtctgag ttacaggaga tgaaccaacg
     3361 gcaaccgtcc cgacgttgtc ggtctggggg acgatttgct tgatggcatg gttggtccag
     3421 tcagagacga gaatggtacc atcgggagcc ggccataccg acaccggacc actaaatccg
     3481 cggccccagc ccgcgacggt gttgctcagc gtggaagtgg cctcacgaat aagattggtg
     3541 ccaaactcgc tgagccacag atttccctgg gcatcgcgag ccaaatctcc cgggccctgg
     3601 aagctatagt tattgagcga actttccgac gtatagggag actgaagctt ctccaggtgg
     3661 tccagattgc catcgatcac aaaaatgttg tcactggcat cgacggcaat tcctcgaggt
     3721 tgcgcaacga cattggccgc aaccactgtg gtaaccacag gctggccgga gctgaacgag
     3781 atctttgaga tggtgttggc gatattgtcg gatacgaaga cgtttcccga cggatcgccg
     3841 gcgatacccc atggcccctt ccaaccggtg ccgatggcca ccggggtggc gaaggttcct
     3901 gaagtattga cccactcgta gacatgcccg gcggtcgtgg aaaagttgcc gaatgccgct
     3961 acccacagat cgccgcggga atcgagtccg aggccgcgca gacagggatc gggttcggtg
     4021 acgggcgatg ggcagacaaa gccgcccgcc ggggaaggca gcgcaacggg gctgccgtag
     4081 ctccccggcc cattgcgggt catttcgtaa accgtggccg tgttcgtcga tgtgttgttg
     4141 tcggcgacga atatattgcc gttgggatcc gtcgtgaccc ccatcggacc tttgaacgaa
     4201 gcgttaatcg tggtggtgat gccgccattg aatgcggcag tctgagcgtg gagcgaggtg
     4261 gcccatgcac aaaggagaat cagcgcactc gcaaccaata acgcgcgaca gcggggctgg
     4321 ctaccggtga agcgggatcg agttgcggag gcgttggagc atccgggggc ggcgttcatc
     4381 aaaacacttc tcctgtggga gggcaaggcc tttcagcaac caggcagcga taccagtcag
     4441 cggtccgaga gccagatctg gagctggttc ggcccgaaaa taacggcttt gtaatgtgtg
     4501 tgcttcgatg gattctaccg atccgatccg tgatttcaat ccccaattgg acagcgagtt
     4561 gcgacattcc tggtatccct tcagtaacca tttcgcgagt taccagtcct gctagaatcc
     4621 gccggaaaat accagttgtg ggcccacgcc cgtgccatag agatacgcgg tcgccagtac
     4681 ggtgccggag gtattcagaa gcttcaccgc gccgtagcgg gtaccggcaa attttggctt
     4741 cacggtgacg tcgaccgtgc aggtagcttt ggacgcgtag ggatggccgg caccattggt
     4801 cgtggtgcag gagccggttc cggcgtcggc aaagtcgagg ccggttgcac cctgggtcac
     4861 gacgaccggt gcctgaatcg tcgtggtgga ggagccggta aaggtgaagg taagcgtctg
     4921 ttttgccgag gtggaaccaa tcgctacggt cccgacattg accacaggcg cgatttgacg
     4981 aatgacgccg tggtcttcat ccgagacgag aatgttgcct tcgaacgttt gccagatttc
     5041 gaccggtgtt tgcaggcccg tgccaaagtt caagaccgtc gcgaagttgc tggcgatggt
     5101 catttcctgg atcaggttcg cgttgtagtt tgtgacccat atgttgccca gattgtccac
     5161 ggtgagattg ccgggcccgc caaaattggc gctgccgata ttgttggccg acgtgtacga
     5221 agacgaagcg gtcagctgca cgacgcgagc gacgttgccg tccaggatca gcagattgcc
     5281 gccggagtcg aaaaccatgc cgccgggctg gtggacactg gtggtgttga tccaggttgc
     5341 ggtgctgccg gtgattttgg cgatggagtt ggcggtttca
//
LOCUS       DASZYN010000345         2653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_15988, whole genome shotgun sequence.
ACCESSION   DASZYN010000345 DASZYN010000000
VERSION     DASZYN010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15988"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..1853
                     /locus_tag="VHE33_08920"
     CDS             48..1853
                     /locus_tag="VHE33_08920"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVT97618.1"
                     /translation="MMHRFFRSPATLAASAGLLLTFTAVVPSIAQSRGSNFVDTQRTA
                     SPYQGTVVEQIVAMVNDQVISKSDYERAERDLKQEAQQQGWSHQQVMEQQRDLLRSLI
                     DKQLLLSKGKELGITGDNEVVKRLDDMRKQYHMDSMEDLQKAAEAQGVSFEDLKEQIR
                     EGIVQSNVISQEVGRRIDITPSEIRQYYNAHLQDFQRPEQVKLSEILIPTPNPDDAAQ
                     VAEAEKKANAVEDRLKSGADFATVAKADSTGPTAAEGGRLGEYKRGDLPKVMEDATFN
                     LQPGQYTEPIRTKQGWLILEVTDHSKGGTQPLEEVQNDIQEKIGMQKMEPAMRAYLAK
                     LRDEAAIQVRAPYTDSGATPNEIKFTEGAYQPPQPKKKKHADRARYRQLAQRGRKETT
                     TADAAAPPAGVPTLDQVNSQKGAPVEVAAPKTEKPGKREKIRFGQAPRETLPAGPTRQ
                     VDAGAGDATAAQSTQQVAESAPTSNTVMTNAAGEVIGDTETEKPKKTRLSDHMKDSKQ
                     RQAAEKAAKKKQKFVEPTFTEAQKAEQQQQQQAVGLNGDTTKQKKPNPAKSGPKRRMG
                     EPEASTEQNSQPASGTSNQPNTPAPTTPPATQPQL"
     gene            1911..>2653
                     /locus_tag="VHE33_08925"
     CDS             1911..>2653
                     /locus_tag="VHE33_08925"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HVT97619.1"
                     /translation="MKDIMVAELASRENREITGFFAAVAKQVRTNREGGRYFALILGD
                     RTGQIDGRMWEIDDAGAFEPGDVVKVRGEVCRFNERLQIKVEKIRRAEAGEYELSDFV
                     PQSQRDIGEMWVELRGWVESFGDPDLKALLRAFLSDEEVARQLREAPAAKGLHHAWIG
                     GLLEHILSLMGMAELAAKHYPEVNRDLLLTGVVLHDIGKLRELRWGTSFDYTMEGQLL
                     GHITIGIGMIEQKIAAIPNFPQAKRLLVE"
BASE COUNT          592 a          757 c          924 g          380 t
ORIGIN      
        1 gcttttcgca gcggttttcg aggcggggcg cagagacgtt gatccctatg atgcatcgat
       61 ttttccgttc tcctgccacg ctggctgcca gcgcgggcct gctgctgacc ttcaccgccg
      121 tcgttccctc catcgcgcag agccgcgggt ccaactttgt ggacacgcag cgaacggcat
      181 cgccctatca gggcacggtc gtcgaacaga tcgtggccat ggtcaacgat caggtgatca
      241 gcaagtccga ctacgaacgc gccgagcgtg acctgaagca ggaggcgcaa cagcagggct
      301 ggtcgcacca gcaggtcatg gagcagcagc gcgatctgct gcgctcgctg atcgacaagc
      361 agctgttgct ttccaaggga aaggaactgg gcatcaccgg cgataacgag gtggtgaagc
      421 gcctggacga tatgcgcaag cagtaccaca tggactccat ggaggatctg cagaaggccg
      481 ccgaggcgca gggcgtctcg ttcgaagacc tgaaggagca gatccgggag ggaatcgtcc
      541 agtccaacgt gatcagccag gaggtggggc ggcgcatcga catcacccct tcggaaattc
      601 gccagtacta taacgcgcat ctgcaggact tccagcggcc ggagcaggtg aagctgagcg
      661 agattcttat ccccacgccg aatcccgatg acgcggcgca ggtggccgag gccgagaaga
      721 aggcgaacgc ggtcgaagac cggctgaaat ccggagcgga tttcgccacc gtggcgaagg
      781 cggattccac cgggcccacc gcggccgaag gcgggcggct gggcgaatac aagcgcggcg
      841 acctgcccaa agtgatggag gacgccacct tcaatctgca gccggggcag tacacggagc
      901 cgattcggac caaacagggc tggctcatcc tggaagtgac cgatcacagc aagggcggaa
      961 cgcagccgct ggaggaagtc cagaacgata ttcaggaaaa gatcgggatg cagaagatgg
     1021 agcccgccat gcgcgcctat ctggcaaagc tgcgcgacga ggccgccatc caggtgcggg
     1081 cgccgtatac ggattcggga gcgacaccga acgagatcaa gttcaccgaa ggcgcctatc
     1141 agccgccgca gccgaagaag aaaaagcatg cggaccgggc ccggtatcgg caattggcac
     1201 agcggggacg caaagagaca acgaccgccg atgcggcagc gccgcccgcc ggcgtgccca
     1261 ctctggacca ggtgaattcg cagaagggcg cgcctgtcga ggtagccgcc ccgaagacgg
     1321 aaaagccagg caagagggag aagattcgct ttgggcaggc gccgcgcgag acgctgcccg
     1381 caggcccgac ccggcaggtc gatgccggcg ccggggatgc gaccgccgct cagtcgacgc
     1441 agcaggtggc ggagagcgcg ccgacgagca acacggtgat gacgaatgcg gcgggcgagg
     1501 tgattggcga cacggagacc gaaaagccga agaagacgcg cttgtccgat cacatgaagg
     1561 actcgaagca gaggcaggca gcggagaagg cggcgaagaa gaaacagaaa tttgtggagc
     1621 cgacctttac cgaggcgcag aaggccgagc agcagcaaca gcagcaggcg gttgggctga
     1681 acggcgacac gaccaagcaa aagaagccga atccggcgaa gagcggtccg aaacggcgca
     1741 tgggcgagcc ggaagccagc actgagcaga attcgcagcc ggcgagcgga acctcgaatc
     1801 agccgaatac gcctgcgccg acgacaccgc cggcgacgca gccgcagctt taacctgcag
     1861 ccttcacggg tcatttattt ccgcgccgca gggcgctgcc aggatcacgc atgaaagaca
     1921 tcatggtggc ggagctggcg agccgggaga atcgggagat caccggcttc ttcgcggcgg
     1981 ttgcaaagca ggtgcgcacc aaccgtgaag gcggcaggta ttttgcgctg atcctcggcg
     2041 atcgcaccgg gcaaatcgac ggacgcatgt gggagatcga cgacgcggga gcgtttgagc
     2101 ccggcgatgt cgtgaaagtg cgtggcgagg tgtgccggtt caacgagcgg ctgcagatca
     2161 aggtggaaaa aattcgccgc gcggaggccg gcgagtatga gctgagcgat ttcgttccgc
     2221 agtcgcagcg cgacatcggc gagatgtggg tggaactgcg tgggtgggtc gagagcttcg
     2281 gtgatccgga tctgaaggcg ctgcttcggg cttttctcag cgatgaagaa gttgcgcggc
     2341 agctgcgcga ggcgccggcc gccaaaggcc tgcatcatgc atggatcggc gggctgctgg
     2401 agcacatcct gtcgctgatg gggatggctg agctggctgc gaagcattat ccggaggtga
     2461 accgcgacct gctgctgacc ggcgtggttc tgcacgatat cggcaagctg cgcgagctgc
     2521 gctggggcac cagcttcgac tacacgatgg aagggcagtt gctggggcac atcaccatcg
     2581 gcatcggcat gatcgagcag aagattgcgg cgatcccgaa tttcccgcag gcgaaacggc
     2641 tgctggtcga gca
//
LOCUS       DASZYN010000346         2881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_205651, whole genome shotgun sequence.
ACCESSION   DASZYN010000346 DASZYN010000000
VERSION     DASZYN010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205651"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VHE33_08930"
     CDS             complement(<1..541)
                     /locus_tag="VHE33_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97620.1"
                     /translation="MSDETLNAPPLTFDPDNPGRNIVRHCRSLTVAGAPCRQAALRGE
                     DYCTIHANREARAFEEPGHIVIPLLEDASAIQLTCTKIVHGVANRRTSPTEANSMLRA
                     CAVAASTLPRPARLKPSAEPEPVQPPFSSIALDENNNFIAPRKPWLDAAGNLAQYDSF
                     VPRFTNQKIDPATAVTERYR"
     gene            complement(955..1278)
                     /locus_tag="VHE33_08935"
     CDS             complement(955..1278)
                     /locus_tag="VHE33_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97621.1"
                     /translation="MSLRIPPIRVTMLRMRVNLSTRPSPRPAHRAGSLPITLTIRPAC
                     GLPGDYECAIDADLLMRMLRRQTDLPGYILESFERNLRTQLDARLPGVELNETVLTDI
                     GYFID"
     gene            complement(1480..2163)
                     /locus_tag="VHE33_08940"
     CDS             complement(1480..2163)
                     /locus_tag="VHE33_08940"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT97622.1"
                     /translation="MDRSLHGLAGAAEWPSLQAMLPDLQQARVLDLGCGFGWFSRYAR
                     EHGARSVFACDVSENMIQRAIAMTSDHAIDYRKLDLEHIELPENSFDLAFSSLALHYL
                     ETLDRLFPLLCRSLVAGGSFVFSTEHPLYTAPRDPAWIVQQDGTRAWPLNQYLAEGPR
                     ITNWLTDGVVKQHRTIATMVNALVRAGFALTQLDEWGPSKEQIAAHPEWAEHRDRPMF
                     LLVAARKAA"
BASE COUNT          513 a          706 c         1075 g          587 t
ORIGIN      
        1 tccggtagcg ctcggtaacg gcggtggcgg ggtcgatctt ctgattggtg aagcggggga
       61 cgaagctgtc gtattgggcg aggttgccgg cggcgtcgag ccagggtttg cgcggggcga
      121 tgaagttatt gttctcgtcg agggcgatgc tggaaaacgg cggctgaacg ggttcaggct
      181 cggcggaggg tttaaggcgc gcggggcgag gaagggtgga ggcggcaacg gcgcaggccc
      241 ggagcatgga gttcgcttcg gtgggtgagg tgcggcggtt ggcgacaccg tgcacgattt
      301 tggtacaggt gagctggata gcggaggcgt cttcgaggag ggggatgacg atgtggcctg
      361 gctcttcgaa ggcgcgcgct tcgcgattgg cgtggatcgt gcagtaatcc tcgccgcgga
      421 gtgcggcctg gcggcagggt gcgccagcga cggtgaggga gcggcagtgg cggacgatat
      481 tgcggccggg gttgtcgggg tcgaaggtga ggggcggtgc gttgagggtt tcgtcggaca
      541 tcgaagacct ttcgtgatgc gagagcgagc aaatgcgagc gaacggcgag tgaacgagcg
      601 agcgcgcgcc ggcgcgtgag gtgcggaggc gatgctcaaa aaagcgaagg gcctgcgcca
      661 ccacgcgccg acccttcgca aagaaccggg agccatcccg gtttgcccat gtcagaacca
      721 ctcacccgaa aaaggaaaag cccgcacgcg gcgggctctc tttctttttc tactctaagt
      781 tcagaatacc aaatccaaag aattatcgtg ccaaactttt ttgggagttt tggcttgtaa
      841 gtggcgagtt atcaggcgtc attactttgg attttgcgcg cggggcttga cagattttcc
      901 agagcgggcg gacggtcgcg agttttgacc tgctgagctg ctgcggcgcc gatgctagtc
      961 gatgaagtag ccgatgtcgg tgaggacggt ttcgttgagt tcgaccccgg ggaggcgcgc
     1021 gtcgagctga gtgcggaggt tccgctcaaa gctctcgagg atatagccgg gcagatcggt
     1081 ttggcggcgg agcattcgca tcagcaaatc ggcgtcgatg gcgcattcgt agtcgccggg
     1141 taagccgcag gcgggacgga tggtcagcgt gatgggaagc gacccggccc gatgggcggg
     1201 gcgaggggag gggcgggtgc tgaggttgac gcgcatgcgc aacatcgtga cgcggatggg
     1261 agggattctc aatgacaccg acagggaatc cgatgcgaac gaaatgggga gggtgatatg
     1321 gccgggatgt tgccgggtat cccgcggagc gtggtctgcg gcgtgcctcg aagttatgct
     1381 cagccccggc gtgaagctat ttgctgtggg ccaggcgttc tacaaaggga agggccggtc
     1441 tggagaggtg gatgggaggc ctggtcaggg tatcgcggct tacgctgctt ttctggccgc
     1501 gaccaggagg aacatgggcc ggtcgcgatg ctccgcccat tccggatgcg cggcgatctg
     1561 ctccttgctg gggccccatt cgtcgagttg agtcagggca aagccggcac ggacgagcgc
     1621 gttcaccatt gttgcaatgg tgcgatgctg cttcacgacg ccgtcggtca gccagttggt
     1681 gatgcgaggg ccctccgcca ggtactgatt gaggggccag gctctggtgc cgtcctgctg
     1741 tacgatccat gcgggatcgc gtggggcggt atagagggga tgttcggtgg aaaacacaaa
     1801 cgaaccgccc gcgaccaggg agcgacacag caaggggaaa aggcggtcca gggtttcgag
     1861 gtagtgcaga gccagagaac tgaaggccag gtcgaagctg ttttcaggca actcgatgtg
     1921 ttccaggtcg agttttcgat aatcgattgc gtggtcggag gtcatggcga tggcgcgctg
     1981 gatcatgttt tccgagacat cgcatgcgaa gacgctccga gcgccgtgct cgcgggcata
     2041 acgggagaac caaccaaatc cgcaaccgag gtcgagaacc ctggcctgct gcaaatcggg
     2101 aagcatcgcc tgcagagacg gccactcggc tgcaccggcg agaccatgca gactgcgatc
     2161 cattcggcag tattcctgga agaaatcagg gtcgtcgtag atgttttgcg tcatcgaagg
     2221 cgagggcaag agtcgtcggc tttctgccga cttctgagtt tacgcaaagt ccccaggctt
     2281 ggtggctggg ttgttccggg cgcttagcgg ccactccctt cccgaaacgg gagagtgaat
     2341 tcggagggat gactttcgat tgaacctgtc gcctggattg cttgatttcc ggacgggaag
     2401 cgattgagct gcttgctgtg gggtgaacct ggcgcgtctg cgattggggt tgcgtttggt
     2461 tatgggcggg cgtgtttccc gatttggtcc gagagggtac gtttggccgg agccgagggc
     2521 gaaatggttt gacatgcggg cgggtgaggc gtgagctgct tgctgtgggg tgaacctggc
     2581 gcctctccga tgggggggtg gattgctttc ggaaagcccg cgccggtcat tgccatcccg
     2641 ccttccggac cctggcgtcc cttatttggc tgactttccc aggaaattcg ctatctcccg
     2701 tgcttactcg cggcgttcct ggaggtagtc ggccgacacg gggttctcgt agagggagta
     2761 gtcgcgggtg acgacggtga ggccgctggg cgtcacgaag tagttcttgc ggtcgtcgtt
     2821 ctggtcgtag ccgatgacgg tgccttcggg cagatgcacg tcgcgatcga taatggcgcg
     2881 g
//
LOCUS       DASZYN010000347         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_205660, whole genome shotgun sequence.
ACCESSION   DASZYN010000347 DASZYN010000000
VERSION     DASZYN010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205660"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VHE33_08945"
     CDS             <1..390
                     /locus_tag="VHE33_08945"
                     /inference="COORDINATES: protein motif:HMM:NF025879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HVT97623.1"
                     /translation="KIPQMWQGAMQNGTLEQIPNRDGDALVVVYSDYASDNTGDYNYT
                     LGYKVTSAEKIPAGMVAKTIQPGKYATLDSEQGPPQQVIPALWQRINSMSPQQLGGAR
                     AYQTDFETYADVTDWSNVRITVHIGLK"
     gene            478..>1586
                     /locus_tag="VHE33_08950"
     CDS             478..>1586
                     /locus_tag="VHE33_08950"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF014839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HVT97624.1"
                     /translation="MSSIQVQLPDGSVREVPSGTTPFSIAESISPRLAQAAVVARIRP
                     LKAAAGSAASAATGEAEMYAAEDTHAERMVDLSTPLQEDVALELLTDKAPEALKVLRH
                     SAAHVMATAVTELFPETKLGHGPATENGFFYDFWRPTPFTPEDLKLIEGRMAEVTARD
                     EKFVRDFEPREKGLEEFKTGNDFMKVHFVEKFTKPGEEISLYRNGKFVDFCRGPHVPS
                     TGRVKAFKVMSLSGAYWLGDEKNPQLQRIYGTAFFSKKDLDEYLERIEDAKRRDHRRL
                     GKELELFTVSEEVGAGLPLWLPKGATIRRLLEDYILGLERELGYEHVYTPDLAKVDLY
                     KRSGHWAHYHEDMFPPMDLETEQMVLRPMNCPHHIL"
BASE COUNT          337 a          550 c          439 g          260 t
ORIGIN      
        1 aagatcccgc agatgtggca gggcgcgatg cagaacggca cgctggaaca gatacccaac
       61 agggacggtg acgcgctggt cgtggtgtac agcgactacg ccagcgacaa taccggcgat
      121 tacaactaca cgctgggcta caaggtcacc tcggcggaga agatccccgc gggcatggtg
      181 gcgaagacca tccagcccgg aaaatacgcc acgctcgact ctgaacaggg gccgccgcaa
      241 caggtgatcc ctgcgctttg gcagcgcatc aacagcatga gtccgcaaca actcggcggc
      301 gcccgcgcgt accagaccga cttcgaaacc tatgccgacg tgacggactg gagcaacgtg
      361 cgcatcaccg tccatatcgg gctgaaataa tcgctccctt cgattgcttc cgcccgatcc
      421 ggccggtatc ccggaagtga cctgtaccct gcaccctcca ccctgtaccc ttttcctatg
      481 agttccattc aggtccagct tcccgacgga tcggtacgcg aagttccgtc cggcaccacc
      541 ccgttctcga tcgcagaaag catttcgccg cgcctcgccc aggctgccgt cgtggcacgc
      601 attcgtccgc tcaaagccgc cgctgggtct gcggcctccg ctgcaaccgg tgaggctgag
      661 atgtatgccg ccgaagatac ccacgccgag cgcatggtcg acctttcgac gccgctccag
      721 gaagacgtcg ccctcgaact gctgaccgat aaggctcccg aagcgctgaa ggtcctgcgc
      781 cattccgccg cccacgtcat ggcgactgcg gtcaccgagc tgttccccga gacgaaactc
      841 ggtcacggcc ctgccaccga aaacggcttc ttctacgact tctggcgacc cacgcccttt
      901 acccccgaag atctcaaact catcgaaggc cgcatggccg aagtcaccgc ccgcgacgag
      961 aaattcgtcc gcgacttcga gccgcgcgaa aaaggcctcg aagaattcaa gaccggcaac
     1021 gacttcatga aggtccactt cgtggaaaag ttcacgaaac ctggcgaaga aatatcgctc
     1081 taccgcaatg gcaagttcgt ggacttttgc cgcggcccgc atgtgccctc gaccgggcgg
     1141 gtgaaggctt tcaaggtgat gagcctgtcg ggcgcctact ggctcggcga cgagaagaac
     1201 ccgcaactgc agcgcatcta cgggaccgcc ttcttctcga agaaggacct cgacgagtat
     1261 ctcgagcgca tcgaggacgc gaagcgccgc gaccatcgcc gcctgggcaa ggagctggag
     1321 ctctttacgg tcagcgaaga agtcggcgcc ggcctgcccc tgtggctgcc caaaggcgca
     1381 accatccgcc gcctgctcga ggactacatc ctcggccttg aacgcgagct cggctacgag
     1441 cacgtctaca cccccgacct cgccaaggtc gacctctaca aacgctccgg ccactgggcg
     1501 cactaccacg aggacatgtt cccgcccatg gacctggaaa ccgaacagat ggtgctgcgc
     1561 cccatgaact gcccgcacca catcct
//
LOCUS       DASZYN010000348         9307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_848959, whole genome shotgun sequence.
ACCESSION   DASZYN010000348 DASZYN010000000
VERSION     DASZYN010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 9307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9307
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848959"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /gene="plsX"
                     /locus_tag="VHE33_08955"
     CDS             complement(<1..875)
                     /gene="plsX"
                     /locus_tag="VHE33_08955"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HVT97625.1"
                     /translation="MLIDIALDAMGSDKAPEPEIRGAILAARNLPVRVHLVGPEPLLR
                     RHLDGAYGHRHLPISIVHTDEYISMDEKAAHAVRTKRNSSMRIGLKMVKEKRAAGFFT
                     AGNTGAGMATAKMTLGALQGVDRPALAIVVPTLSGSPCILLDAGANVDCKPQNLEQFA
                     LMGELYARSVLRIPRPRVGILSVGEEEGKGNELTREATLLVRKLPINFIGNVEGRDIY
                     NGNCDVIVCDGFVGNVALKTSEGLSRLVREMLKASLTQTVTAQVGALLSRRAFNAFRR
                     RLDPSEYGGAPLLGVR"
     gene            complement(1140..1790)
                     /locus_tag="VHE33_08960"
     CDS             complement(1140..1790)
                     /locus_tag="VHE33_08960"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HVT97626.1"
                     /translation="MRAKGQYLTWSGSGHNLEVVPTAKDISMLITLLDLEREPVHFDL
                     KFSPGTIDYGEEATQTSDLTVAGQAELLKEHRGPKEVVPDIRVRAKWSGTFEAPCARC
                     LEAVRHELKGDFDLLFRPLGVDAGPAERALVVPETEIGYYQEGGLALEDVLREQVLLS
                     LPARTLCSADCRGLCPRCGRNLNEGACNCEAPPADERWSALSDLRSRLSPETKETH"
     gene            complement(2246..2794)
                     /locus_tag="VHE33_08965"
     CDS             complement(2246..2794)
                     /locus_tag="VHE33_08965"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVT97627.1"
                     /translation="MKRRRFLQSAAAVFPLSWLERAGGAGMVGGESAPAHVIPAGQDR
                     LGEHHSLGFSTIAFKVLPRETAGGLFLIEHTHLQKAGPPLHLHHAQEEYFYVMEGRAL
                     FQVGEQKVELKQGESVLGPRGVPHTFMPVGESPARMLIAFTPAGQMEEFFRDQEKSGA
                     PFKDPEDFARYGMTWLGPPLKA"
     gene            complement(2900..3346)
                     /locus_tag="VHE33_08970"
     CDS             complement(2900..3346)
                     /locus_tag="VHE33_08970"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT97628.1"
                     /translation="MRADADVVVRELRGDDAAAVAELSGQLGYPGTAEEVRKRIEAMT
                     RSGDRVAFAAVRAAELVGWIDAAVERHLQADDAVVIGGLVVGETERGCGVGKRLCEEV
                     ERWAGERGFARVRVRSQIKRADAHRFYLRDGYRVVKTSLVLEKELD"
     gene            complement(3428..4408)
                     /locus_tag="VHE33_08975"
     CDS             complement(3428..4408)
                     /locus_tag="VHE33_08975"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HVT97629.1"
                     /translation="MMENDQDLEQALYAAFARDPAPATLMAGVEERLAADSASGGLGS
                     MQPAMAGGRVFVPTFAGLEIRTQSRWTSLLSVGTHGLICAIIALFAIAQWHTRQVQKK
                     MVATAVTPFMPASNLKDLMGGGGGGGNHELLEASKGKLPEFAKKQIAPPQILRLDNPK
                     LAVEPTVVMPEIKLPDATNMPDMGIPQSAQVAFASQGSGSGSGFGSGSGGGVGSGRGN
                     GIGPGEGGGYGGGVYHVGGGVSNPTLVFAPDPEFSDEARRAKYQGVCVVGLIVDVTGN
                     PQHVHIVRALGMGLDEKAMEAVRQYKFKPAQFKGKNVAVEVNIEVNFRIY"
     gene            complement(4447..4917)
                     /locus_tag="VHE33_08980"
     CDS             complement(4447..4917)
                     /locus_tag="VHE33_08980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVT97630.1"
                     /translation="MMLEHQSMVFSIALRILGDRFLAEETAQDVFVELHAKLGELKGD
                     EHVVHWLRRVTVHRSIDRLRERARRREEVTDPGALPELVVEGTPGDPLLSRQIRQLVG
                     SLPVIQRTVITLRYQEDMSVEEIGEALSMPAATVKSHLRRTLQLLREKMTRVLH"
     gene            5148..5627
                     /locus_tag="VHE33_08985"
     CDS             5148..5627
                     /locus_tag="VHE33_08985"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HVT97631.1"
                     /translation="MTNEELLAGAALKSWKLVLGRFDKDLANLSDADFQKRVAPGRNR
                     LYYLYGHLTAVHDRLLPMLGIGERLHPELDEAFISSPDGATSDSVSVADLRAAWTDVN
                     ARLTAAFESYSPQDWLRKHTAVSDEDFVADPTRNRLAVVLSRTNHLSFHTGQFVLAK"
     gene            5681..7420
                     /locus_tag="VHE33_08990"
     CDS             5681..7420
                     /locus_tag="VHE33_08990"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HVT97632.1"
                     /translation="MPGFAFFVRSLRPPAALLFSAALVLAAAAHAQTTPSQPTPAQAP
                     APTTAAQTQRPRPTDAVAALPDRTMMLRGAYGPFRANNDLLYYHLTVRVDPTNQTLKG
                     KNTIRFRMLHDASRIQLDLQEPLQIDKILLGSTELKYRRDSGAVFIDFPQTLHTGQTY
                     SIDFYYSGHPRTTGRFGGITFKTDPAGHTWINTACEGIGASLWWPNKDQWRDEVESMD
                     ISVEIPDNLTDVSNGKFVAKTDLGDGYTRWDWHVSYPINNYDVSLNIGEYAHFSDKLG
                     TLALDFYALPADLDKAKTQFAQAKGMIEAYQHYFGEYPFAKDGYKLIEAPYSGMEHQS
                     AVTYGNRFENGYLGRDWTGVGISPRFDFIIVHESGHEWFGNSVTAADPADMWIHEGWT
                     TYLESLYVEYRWGTADAMKYLSGYKPKVQNLRPILGERGVNAEPPQDQYFKGALMLNT
                     LRSVVNNDAAWWKLLHDLYQHFKYQNILTEDIVAFCNQHTGMNLTPVFNQYLRHTAIP
                     RLDLLFGEEPNTVLYKWRADEQDFAMPVRVGTAGHWQTIHPTTHWQSMQTPLSKDDFA
                     VDTDHFFIDVNKQ"
     gene            complement(7506..>9307)
                     /locus_tag="VHE33_08995"
     CDS             complement(7506..>9307)
                     /locus_tag="VHE33_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HVT97633.1"
                     /translation="LAAKEGLAKRRPSVELSMGAGRQKVNEVEDVLGKIVQTKTDHAK
                     KDADDMDAMYSHGRSFMLGLMIAGVILGVGLGILIARSIARPLSKVAEAAKQIALGDV
                     NQTIDYHGGDEVGSLADSFRGLVEYIQGVAVALEKMAAGDLNTAVEAKSERDVLSRSY
                     LKTVEAIQTLDKMFARLTDAARNGNLAERGHPERVQGAYSEMVKGANALLEALIVPLQ
                     VSAKYVDRISKGDIPEKITEEYKGDFNQIKNNLNTLIDAMHAVTAAAEKIAHGDLTVE
                     VKERSAEDKLMQALAAMVLGLTRTVGEIRTIAGEVASASQAISTASVQVSKGASSQAA
                     AAEEASSSMEEMVSNIKQNADNAQQTDKIANKSAKDAQESGKSVQEAVSAMKEIANKI
                     SIIEEIARQTNLLALNAAIEAARAGEHGKGFAVVAAEVRKLAERSQKAAAEINHLSGT
                     TLTVSEKSGEMLAKLVPDIQRTAELVQEITAASKEQDTGAEQINKALQQLEQVIQQNA
                     SAAEEMASTTEELTGQSEQLVSALGFFQTKDEAGGVRKGPRVTEPTAAELSDAPVRNG
                     NGHGLRSKVLSRAGVKLQLEEKKDRLDEEFERY"
BASE COUNT         1754 a         3552 c         2197 g         1804 t
ORIGIN      
        1 cgcacgccca gcagcggcgc cccaccgtac tccgacgggt ccagcctgcg tcgaaacgca
       61 ttgaacgctc gccgtgaaag cagcgcgcca acctgcgccg tcaccgtttg cgtcagcgat
      121 gccttcagca tctcccgcac cagccggctc agcccctccg acgtcttcag cgcgacattc
      181 cccacaaacc catcgcacac gataacgtca cagttgccgt tataaatatc ccggccctcg
      241 acgtttccaa tgaagttgat cggcagcttg cgtaccagga gcgtggcttc gcgggtcagc
      301 tcgttgcctt tgccttcttc ctcaccaacc gaaagaatcc ccacacgcgg ccgcggaatc
      361 cgcagcacgc tgcgcgcata cagctcgccc atcagggcaa actgctccag attttgcggc
      421 ttgcagtcga cgttcgcgcc ggcatccagc aagatgcacg gcgaacccga caacgtcggc
      481 accacaatcg ccagcgccgg ccggtccact ccctgcaacg cgcccagcgt catctttgcc
      541 gtcgccatcc ctgcgccggt gttgcccgca gtgaagaatc ccgccgcgcg tttttccttc
      601 accatcttca gcccaatgcg catcgagctg ttgcgcttgg tccgcaccgc atgcgccgcc
      661 ttctcgtcca tgctgatgta ctcatcggtg tggacgatcg aaatgggcag atgacggtgt
      721 ccgtaggctc catccagatg acgccgcagc aacggctccg gccccaccag atgcacccgc
      781 accggcagat ttcgagcagc aagaatcgcg cctcgtatct caggctccgg agctttgtcc
      841 gaacccatag cgtcgagagc gatgtcgatg agcatcgagc gctggttact tggtttcctt
      901 tacttccaga acttcgcgcc ccttatagta gccacacttg gggcaggcgc gatggggaag
      961 tttccgctcg tggcagttcg gacactccga taaagaattc gcagtcagcg cgtcatgcgc
     1021 gcgccgcgtg gaagtgcggg ccttcgagtg gcgccgcttt ggattcggca ttgcagattc
     1081 ctttcgatcc cggcatcaga ttccggggca aaaacgtcag tgttcgaccg gttctctctt
     1141 cagtgcgtct ctttcgtctc cgggctcagc cggctgcgca aatccgaaag cgccgaccat
     1201 cgctcatcgg ccggcggggc ctcgcaatta caggctccct cgttcagatt acgtccgcag
     1261 cgggggcata gccccctgca gtccgcactg caaagtgttc tagccgggag acttaacagc
     1321 acctgctccc gcagaacatc ctccagcgcc aaaccgcctt cctgataata accgatttca
     1381 gtctccggca cgaccagcgc gcgctccgcc ggaccagcgt cgacacccag cggccgaaac
     1441 agcagatcga aatcgccctt cagctcgtgc cgcaccgcct ccaggcaccg cgcgcacggg
     1501 gcctcgaagg tccccgacca cttcgcgcgc acgcgaatat ccggcaccac ttccttcggc
     1561 ccgcgatgtt ctttcagcag ctccgcctgc cccgcaaccg ttaggtccga ggtctgcgtc
     1621 gcttcctctc cgtagtcaat cgttcccgga gaaaacttca ggtcaaaatg taccggctcg
     1681 cgttccagat cgagcagggt aattagcatc gaaatgtcct tcgccgtggg gactacttca
     1741 aggttatggc cacttcccga ccaggtcaaa tactgtccct tcgcgcgcat ccataaaaca
     1801 tttcgccccc cggcggtcga agccggactc gccaataatc tctggaccat cgaagaaata
     1861 gttggcgtgg tcgaaaactc agtcaaagat caccggcgtc gagaatttcg aggaagaact
     1921 tcttggcccg gtcgaaaatt cagaggatca ccgtttcgga aggccaggtt caccccacag
     1981 caagaagcgg atactgtcgg ccgccgattt tcaattcaaa tcagcccccc aagcactcaa
     2041 aagtaccgcg ccgactccag cccgtcccat ttcgggagcc gcaccaacac ccgccccaac
     2101 agcattggaa aggccaggtt caccccacag caagaagcgg ataccttcgc cacgcgattt
     2161 tcaattcaaa tcagccccgc actcactcga aagtacttcg cagaatcaaa cgcgtcccat
     2221 ttcgggaagg tcacccgtca ttcccttatg ccttcagcgg cggccccagc catgtcatcc
     2281 cgtaccgcgc aaagtcctcc ggatccttaa acggcgcacc cgacttctcc tggtcccgga
     2341 aaaactcctc catctgtccg gccggcgtaa acgcgatcag catccgcgcc ggcgactcac
     2401 ccacgggcat aaatgtatgc ggcacaccgc gcggccccag caccgactcg ccctgcttca
     2461 gttccacctt ctgctccccc acctggaaca gcgcccggcc ctccatcaca tagaagtact
     2521 cctcctgcgc atggtgcagg tgcagcggtg gtcccgcctt ctgcagatgc gtgtgctcga
     2581 tcagaaacaa gccgcccgcc gtctcgcgcg gcaacacctt gaacgcaatc gtgctgaacc
     2641 cgagactgtg gtgctcgccg agccggtcct gacccgccgg aatcacatgt gccggcgcgc
     2701 tctcgccgcc caccatccca gccccgcccg ctcgctccag ccagctcaac ggaaacaccg
     2761 cggccgccga ctgcagaaac cgccgtcgct tcatgctgtg tcctctttca cgcctaaaag
     2821 atgggggaca attggccgga agtgtagcat gtccctgttt gcgctcgccc gctcgtcagc
     2881 gattccgacc gccgccgact cagtccagtt ccttctccag cacgagtgac gttttcacca
     2941 cccgataccc atcccgcaga tagaaccggt gagcatccgc acgcttgatc tgcgatcgca
     3001 cccgcacccg cgcgaacccc cgctcccccg cccaccgctc cacctcttcg cagagtctct
     3061 ttcccactcc gcagccccgc tccgtctctc caactaccag cccgccaatg accaccgcat
     3121 cgtcagcctg cagatgccgc tccaccgccg cgtcgatcca ccccaccagc tccgccgcgc
     3181 gcaccgccgc aaatgcaaca cgatctcccg accgcgtcat cgcctcgatg cgcttccgta
     3241 cttcctctgc tgttcctgga tacccaagct gaccgctcag ctccgccacc gcagccgcat
     3301 cgtcacccct cagctcccgc accaccacat ccgcatccgc ccgcatgcgg tcattatccc
     3361 gcaccgcgac gcgggctccg cgaactcaac cctgcgacat gcgccctgcg acctgtgccc
     3421 tgacccctta gtagatcctg aagttcactt cgatattcac ttccaccgcc acgttcttcc
     3481 ccttgaactg cgcgggcttg aacttgtact gccgcaccgc ctccatcgcc ttctcatcaa
     3541 gccccatccc cagcgctcgc acaatgtgca cgtgttgcgg attcccggtc acgtccacaa
     3601 tcagtcccac cacacacaca ccctgatact tcgctcgccg cgcctcatcg gagaactccg
     3661 ggtccggcgc aaaaaccagc gtcggattcg aaactccgcc tccaacgtga tacacaccgc
     3721 cgccgtaacc cccgccttcg cctggcccaa tgccgttgcc ccttcccgac ccgactcctc
     3781 ctcccgaccc cgatccaaat cccgagcccg aacccgatcc ctgggaggca aacgccacct
     3841 gcgccgattg cggaatcccc atgtcaggca tgttcgtcgc atccggtagc ttgatctccg
     3901 gcatcacgac tgtcggctcc acggccagct tcgggttatc cagccgcaga atttgcggcg
     3961 gtgcgatctg cttcttcgca aactccggca gcttcccttt cgacgcctcc agcagctcgt
     4021 gatttccccc gcctccgccc cctcccatca gatccttcaa attcgaagcc ggcatgaacg
     4081 gagtcaccgc cgtcgccacc atcttcttct gcacctgccg ggtgtgccac tgcgcgatcg
     4141 caaacagcgc gataatcgcg caaatcaaac cgtgcgtccc caccgacagc aggctcgtcc
     4201 accgcgactg cgtacgaatc tccaaacctg cgaatgtcgg cacaaacacc cgcccgcctg
     4261 ccatcgccgg ctgcatcgaa cccaggccac ccgatgccga atcggccgcc agccgctctt
     4321 ccacacccgc catcagcgtg gccggcgcgg gatcgcgtgc aaacgctgcg tacaaagcct
     4381 gttccagatc ctgatcgttt tccatcatcg tccgatcctc acctgactca tcgtccctgc
     4441 acgcctctaa tgcagaaccc gggtcatctt ctcgcgcaac agctgcagcg tgcgtcgcag
     4501 gtggctcttc accgtcgccg ccggcatgct cagcgcttcg ccaatctctt ctaccgacat
     4561 atcttcctgg tatcgcaagg tgattaccgt gcgctggatc accggcagcg atcccaccag
     4621 ctgccggatc tgccggctta acagcggatc gcccggcgtc ccctccacaa ccagctccgg
     4681 cagtgccccc ggatccgtca cctcttctct tcgccgagcc cgctcccgga gccggtcaat
     4741 cgaccggtgc acggtcaccc gccgcagcca gtgaacgacg tgctcgtcgc ccttcaattc
     4801 gccgagtttc gcgtgcagct caacaaacac atcctgcgcc gtctcttctg ccagaaagcg
     4861 atcccccagg atacgcagag caatgctgaa caccatcgat tggtgctcca gcatcaacgt
     4921 tcgaaaggac tgctcaggag ctttcagggc gactttcctg ccttcacctg gtatacgctc
     4981 gccaccacgg ccccggatgc agaaatttgg tgcctctctt ctggcggtta cccacgcgcg
     5041 agtaccgaag agttaactgc gcctccgcgc cggggcacct ctttccgcat ccagccgcct
     5101 cgggatacat ccaaccaaca cctttctcac tcgggagacc tatcgcaatg accaacgaag
     5161 agcttctcgc cggcgccgca ctcaaatcgt ggaaactggt cctcggccgc ttcgacaaag
     5221 acctcgccaa cctctccgac gctgactttc agaagcgcgt cgcgcccgga cgcaaccgcc
     5281 tctactatct ctacggtcac ctcacggctg tgcacgaccg cctgctcccc atgctcggca
     5341 tcggcgaacg ccttcatccg gaactcgacg aagctttcat ctccagtccc gatggagcca
     5401 cgagcgattc cgtctccgtc gccgaccttc gcgcagcctg gacagacgtc aacgcccgcc
     5461 tgactgcagc ttttgaaagc tattcccctc aggactggct ccgcaaacac acagccgtct
     5521 ccgacgaaga cttcgtcgct gaccctaccc gcaaccgcct cgccgtggtc ctcagccgca
     5581 ccaaccacct cagctttcac accggccagt tcgtcctcgc aaaatagcgc ttccgcgatg
     5641 gcagccagcg caatcccctt gctgctttac gatggctgcc atgcccggtt ttgcgttctt
     5701 cgttcgctcc ctgcgtcccc ctgccgccct tctcttctcc gcggcgttgg tcctggccgc
     5761 cgctgcccac gcgcaaacga cgccaagcca gcccaccccg gcgcaggcgc cggccccgac
     5821 caccgcagcg caaacgcagc gtccccgacc caccgacgcc gtcgctgccc tccccgatcg
     5881 caccatgatg ctgcgcgggg cttacggccc cttccgcgcc aacaacgacc tcctctacta
     5941 ccacctcacc gtccgcgtcg atcccaccaa ccagaccctc aaaggcaaaa acaccatccg
     6001 cttccgcatg ctccacgacg cctcccgcat ccagctcgat ctccaggaac ctctccagat
     6061 cgacaaaatc cttcttggat ccaccgagct caaatacagg cgcgactccg gcgccgtctt
     6121 catcgatttc ccgcagaccc tgcacaccgg ccaaacctac tccatcgact tctactactc
     6181 cggccatccc cgcactaccg gccgttttgg cggcatcacc ttcaaaaccg accccgccgg
     6241 ccacacctgg atcaacacag cctgcgaagg catcggtgcc agcctgtggt ggccgaacaa
     6301 agatcagtgg cgcgacgaag tcgagagcat ggacatcagc gtcgagattc ccgacaacct
     6361 caccgatgtc tccaacggaa aattcgtcgc caaaacagac ctcggcgatg gctacacccg
     6421 ctgggactgg cacgtctctt accccatcaa taactacgac gtctccctca acatcggcga
     6481 gtacgcccac ttctccgaca agctcggcac tcttgccctc gacttctacg ccctgcccgc
     6541 cgacctcgac aaagccaaaa cccagttcgc tcaggccaaa ggcatgattg aggcctacca
     6601 gcattacttc ggcgaatacc ccttcgccaa agacggctac aagctcatcg aggcgcccta
     6661 ctccggcatg gagcaccaga gcgccgtcac gtacggcaat cgatttgaga atggctatct
     6721 gggccgcgac tggaccggcg tcggcatcag cccccgcttc gacttcatca tcgttcacga
     6781 gagcggccat gagtggttcg gcaacagcgt caccgccgcc gatcccgccg acatgtggat
     6841 ccacgagggc tggaccacct acctcgaatc gctctacgtc gaataccgat ggggaaccgc
     6901 cgacgccatg aagtacctca gcggatataa acccaaggtt caaaacctgc gccccatcct
     6961 cggcgagcgc ggcgtcaatg ccgagcctcc tcaggatcag tacttcaagg gcgcgctcat
     7021 gctcaacacc ctgcgcagcg tcgtcaacaa cgacgccgcc tggtggaagc tcctccacga
     7081 cctctaccag cacttcaaat accagaacat cctcaccgaa gacatcgtcg ctttctgcaa
     7141 tcagcacacc ggcatgaacc taacccccgt cttcaatcag tacctgcgcc acacagcgat
     7201 cccgcgcctc gatctcctct tcggcgaaga acccaatacc gtcctctaca agtggcgcgc
     7261 cgatgagcag gacttcgcca tgcccgtgcg tgtcggtacc gcgggccatt ggcaaaccat
     7321 ccatcccacc acccactggc agtccatgca gaccccgctc agcaaagacg acttcgcagt
     7381 cgacaccgac cacttcttca tcgacgtgaa caaacagtag cctgaatgag gtacaggaga
     7441 gacaactgac acgtctctcc tgcagttctc atcctcccgc gaccgctgta atccgtccgc
     7501 ccacgttagt agcgctcaaa ctcctcatcc agccggtcct tcttctcctc cagctgcagc
     7561 ttcacaccgg cccggctcag caccttcgac cgcaatccgt gcccgttgcc attcctcacc
     7621 ggcgcgtcag acagttccgc tgccgtcggc tccgtcaccc tcggcccttt cctgactcct
     7681 ccagcctcgt ctttcgtctg gaaaaaccca agcgcactca ccagctgctc ggactgcccg
     7741 gtcagttcct cggtcgtcga tgccatctcc tcggccgccg aagcattctg ctgaatcacc
     7801 tgctccagct gctgcagcgc cttgttgatc tgctccgccc ccgtgtcctg ctccttgctc
     7861 gccgccgtga tctcctgcac cagttcggcg gtccgctgaa tatccggcac cagcttggcc
     7921 agcatctccc cggacttttc tgaaaccgtc agtgtcgttc cagaaagatg attgatctcg
     7981 gccgctgcct tctggctgcg ctcggccagc ttcctcacct cggctgccac cacggcgaat
     8041 cccttgccat gctccccggc tcgcgctgct tcaatcgccg cattcagcgc cagcaaattg
     8101 gtctggcggg cgatctcctc gatgatcgag atcttgtttg cgatctcctt catcgccgag
     8161 acagcttcct gaaccgactt tccgctctcc tgcgcatctt tggcgctctt gtttgcgatc
     8221 ttgtcggtct gctgcgcgtt atccgcattc tgcttgatgt tcgaaaccat ctcctccatc
     8281 gacgacgaag cctcttcggc cgctgccgcc tgggaactgg cgcccttcga cacctgcacc
     8341 gacgctgttg aaatcgcctg gctcgccgac gccacttctc cggcaatggt gcgaatctcg
     8401 cccaccgtcc gcgtcagccc cagcaccatc gccgccagtg cctgcatcag cttgtcttct
     8461 gctgaccgtt ccttcacctc cacggtcaga tcgccatgcg cgatcttttc cgccgccgcc
     8521 gtcaccgcgt gcatcgcgtc gatcagcgta ttcaggttgt tcttgatttg attgaagtcg
     8581 cctttgtatt cctcggtgat cttctccgga atgtcgccct tgctgatccg gtccacatat
     8641 tttgccgata cctgcagagg cacgatcagc gcctccagca gcgcattcgc gcccttcacc
     8701 atctccgagt aggcgccctg cacccgctcc ggatgcccgc gctcggccag gttgccgttc
     8761 cgcgccgcat cggtcagccg cgcaaacatc ttgtccagag tctggatcgc ttctaccgtt
     8821 ttcagatagc tccggctcag cacgtcccgc tccgatttcg cttccaccgc cgtgttcaga
     8881 tcgccggccg ccatcttctc cagcgccacc gcaacgccct ggatgtactc caccagcccg
     8941 cggaacgaat cggccagcga gcccacctcg tcgccgccgt gatagtcgat cgtctggttc
     9001 acgtctccca gcgcgatctg cttggccgcc tccgctacct tgctcagcgg tcgcgcaatg
     9061 ctgcgcgcaa tcaggatgcc cagcccaaca cccaggatca caccggcaat catcagcccc
     9121 agcatgaagc tgcggccatg cgagtacatg gcgtccatat cgtcggcatc cttcttggcg
     9181 tgatccgtct tggtctgaac gatctttccc agaacgtctt ccacctcgtt gaccttctgc
     9241 cggcccgcgc ccatcgaaag ttccaccgaa ggccgcctct tcgccagtcc ttctttagcc
     9301 gccagcg
//
LOCUS       DASZYN010000349         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_592429, whole genome shotgun sequence.
ACCESSION   DASZYN010000349 DASZYN010000000
VERSION     DASZYN010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592429"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(873..1103)
                     /locus_tag="VHE33_09000"
     CDS             complement(873..1103)
                     /locus_tag="VHE33_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97634.1"
                     /translation="MTATVDDIYAQAELKAAANPPGRVLATVILAVFTAIGWCCGKLW
                     TGIWFTCMAFMYGVRQGSGVPRPVTPPPAVDH"
     gene            complement(1124..1204)
                     /locus_tag="VHE33_09005"
     tRNA            complement(1124..1204)
                     /locus_tag="VHE33_09005"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(1208..1290)
                     /locus_tag="VHE33_09010"
     tRNA            complement(1208..1290)
                     /locus_tag="VHE33_09010"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1256..1258),aa:Ser,seq:gct)
BASE COUNT          323 a          501 c          480 g          263 t
ORIGIN      
        1 cgcactgtcc gacagcagct tgacgccggc gtacagggca ggcagcccca tcgcctcctg
       61 cgggccgtac tgctggctga ccgggtggac cgggccgccg gaggcaaagc tgccgcccgt
      121 gacccagctg tcccaggggc gttaatcgcc agggcactcc gccgattgtc cgctgctctg
      181 ccgcactagc gcgaatattc tcgatgagcc ccatagcccg ctcacctccc ccgcgcttgg
      241 cacattcttg caatccacct attgttgtgt cgtggaggat ctcacgggct tgacattttc
      301 ccgtctaacg gtactggggc gcgcaccttc ccgcaataga agtagctact ggcagtgcct
      361 gtgtacctgc ggaaacgagt gccaggcgac cacgtcaatg ctcaagaacg gtcgccataa
      421 aagctgcggt tgcctgcgca gagaagcact catagaccgt tccaccattc acggccagaa
      481 gccccgggaa aaagcaagtg ctacatacaa ctcctggagc aacatgctcc ggcggtgcga
      541 gaacccgggt cacccgcgct ggaaagactg ggggggccgg gggattacgg tttgcgcaag
      601 gtggcatact tttgcgtact ttctggcgga catgggggag aagccaccgg gcaagactct
      661 cgaccggatc gacaacaacg gaaactacga gcccgggaac tgccggtggg caacgcctgc
      721 ggaacaggca gcaaaccagc gggggtggaa gctgaccccg gaagtgataa cccgggtcag
      781 agcactgcgc gaagagggcc ttacgatgtc agctatcggc actgccgtgg gcttgaaccg
      841 gcataccgtg gctaaagcgc tgttcccggc gactagtggt caaccgcagg tgggggcgtt
      901 accgggcggg gcaccccgga cccctggcgc acgccgtaca tgaacgccat gcaggtgaac
      961 cagatccctg tccacagctt gccgcagcac cagccgatgg ccgtgaagac cgccaggatc
     1021 accgtcgcca gcacgcggcc cggcgggttg gcggcagcct tcagctcagc ctgagcgtag
     1081 atgtcatcga ctgtggcggt catagctgca gcgtacgcct tagaggaagg tagaggaatc
     1141 gaacccccgg gtgttacccc gcccagcttt tcgagagctg tttgcacccc gtgcggtacc
     1201 ttccgcgcgg agggtgcagg gatcgaacct gcgcacgtca ccgtgactac gggatagcag
     1261 cccgccgcct tgccactcgg ccaaccctcc agtgcgttcc gcgttacgaa cgggaactgc
     1321 tacttgagga tgatgaccgg aagacgcgca tgacctcagt gtacagcaca tgaaacagcc
     1381 cctccagtgg tcagctgaag gggccgtccg acgattctcc ggcaagaaac gagaccatgc
     1441 tgttatcgtc tgcatctaac aggccgcctg gcggggcgaa taccggcaag tttccttccc
     1501 cgcctgccgg cacagtcaac gagcacagga tacagatgat ctaccagctc agcgcacgtt
     1561 tcccgca
//
LOCUS       DASZYN010000350         1960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_849128, whole genome shotgun sequence.
ACCESSION   DASZYN010000350 DASZYN010000000
VERSION     DASZYN010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849128"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..351)
                     /locus_tag="VHE33_09015"
     CDS             complement(88..351)
                     /locus_tag="VHE33_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97635.1"
                     /translation="MNVEFEQFGGSKFLKIRPGCYVRIRDISGVNTGLLSGDDGSELE
                     DRSTVVVHVTSGMFATFEACDTEEEAVEMADKLIQAIENADGE"
     gene            complement(362..844)
                     /locus_tag="VHE33_09020"
     CDS             complement(362..844)
                     /locus_tag="VHE33_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97636.1"
                     /translation="MSNISAVRAAIASQVGTYAYPQLRSLTDPYGQINPPTGIVVYGR
                     PYVNYVTTLQGATAFGGVLGADGPDAPMSPTEVGLDYLILVSQASTVERFETALDLWL
                     GLENDGTAVSVAAAISKDPTLGGTVQWCLPTTVDPPGPVSWNGTEYFGARLHLTVSLL
                     "
     gene            complement(879..>1960)
                     /locus_tag="VHE33_09025"
     CDS             complement(879..>1960)
                     /locus_tag="VHE33_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97637.1"
                     /translation="KPKGLQLSAAQWKAYGAAYSASAKAGYATLAAQNRAIALHNAAN
                     TLQASRLQAALSLQKKVAVAHANARTAAIASYAASQTYRQSILAHQNIALKTRIYADF
                     ERHVLLAGRLQYIYQGEKVYAHNAVMRTLTSQQALNTEEQRFARASKTAKAAARSVAP
                     GKASTGAKSAQALADKAAVNTIKAQAKAAGMAAALAVPPAPKKKTAAKKSRTAPRPQT
                     LAPLKFLHGFGNPDGEDCVAAAIANHLLCDKGIRLSPAQYLALTETVPDGSSIEDALF
                     RVMVSPPWKDGPNLIGYCEVPFCPSRVLVLGYEAEEGPHAAASVGNGFVLSWGEVLQL
                     KATQAAGAQIEECWNLAWIRHRTTL"
BASE COUNT          295 a          663 c          634 g          368 t
ORIGIN      
        1 gactttgctc tcgaagcgcg cccggtcccg gtcagttagc tcgtactcca cagcggcagc
       61 ctacactgaa cagacgtggg tgtaatctta ctccccgtcc gcgttctcga tcgcctggat
      121 cagcttgtcg gccatctcga ccgcttcctc ctcggtgtcg cacgcctcga aggttgcgaa
      181 catcccgctg gtcacatgga ccacgacggt gctgcggtcc tcaagctcac tgccgtcgtc
      241 cccgctgagc aggccggtgt tgacgccgga gatgtccctg atccgcacgt agcagccggg
      301 cctgatcttc aggaacttgc tgccgccgaa ttgctcgaat tccacattca taactgttag
      361 tttacagcag tgacacagtc aggtggaggc gggcgccgaa gtactccgtg ccgttccagg
      421 acaccggccc cggggggtcg accgtggtcg gcaggcacca ctgaacggtg ccgcccaggg
      481 tcgggtcctt cgagatagcc gccgccaccg acaccgccgt gccgtcgttc tccaggccca
      541 gccacaggtc aagggccgtc tcgaagcgct caaccgtact cgcctgggac accaggatca
      601 ggtagtccag gcccacttcg gtcggggaca tgggcgcgtc cgggccgtct gccccgagca
      661 cgccgccgaa cgcggtcgca ccctgcagcg tggtcacgta gttcacgtac ggccggccgt
      721 agaccacgat cccggtgggc gggttgatct gcccgtacgg gtcagtcagg gaccgcagct
      781 gcgggtaggc gtacgtgccg acctggctgg cgatggccgc ccgcaccgcc gagatgttgg
      841 acatggcgca agcgtaaccc cttactgcca cgtggcagtc agagcgtggt ccggtgcctg
      901 atccaggcca ggttccagca ctcctcgatc tgagcgccgg ctgcctgggt cgccttcagc
      961 tgcagcacct cgccccagct gagcacgaag ccgttgccga ccgacgccgc cgcgtgcggg
     1021 ccttcttctg cctcatagcc cagcaccagc acccgggacg ggcagaaggg cacctcgcag
     1081 tagccgatca ggttcggccc gtctttccac ggcgggctga ccatgaccct gaacagcgcg
     1141 tcctcgatgc tggagccgtc agggacagtt tccgtcaggg ccaggtactg cgccggggac
     1201 agccggatgc ccttgtcgca cagcaggtgg ttcgcaatgg cagcggcgac gcagtcctcc
     1261 ccgtccgggt tgccgaagcc gtgcaggaac ttcagcgggg ccagggtctg cggccggggc
     1321 gcggtccggc tcttcttcgc cgccgtcttc tttttcggag ccgggggaac cgcaagggcc
     1381 gctgccatcc cggcggcctt agcctgagcc ttgatggtgt tcacggctgc cttgtcggcc
     1441 agcgcctgtg cggatttcgc acccgtgctg gcctttcccg gggccactga ccgggctgct
     1501 gccttcgccg tctttgacgc cctggcgaac cgctgctcct cagtgttcag ggcctgctgg
     1561 gaggtcagcg tgcgcatcac cgcattgtgg gcgtacacct tctcgccctg gtagatgtac
     1621 tggaggcgcc cggccagcag cacgtgccgc tcgaagtcag cgtagatccg ggtcttcagc
     1681 gcgatgttct ggtgcgccag gatggactgc cggtaggtct ggctggcggc ataagaggca
     1741 atcgcagcag tacgtgcatt cgcgtgtgca actgcaactt tcttctgcaa gctcaaagcg
     1801 gcctggaggc gggacgcctg cagcgtgttc gccgcgttgt gcagcgcaat agctctgttc
     1861 tgggccgcca gggtggcgta cccggccttg gccgacgcgc tgtaggcagc tccgtaagcc
     1921 ttccactgcg ctgcactgag ctggagaccc ttgggcttag
//
LOCUS       DASZYN010000351         2072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_915292, whole genome shotgun sequence.
ACCESSION   DASZYN010000351 DASZYN010000000
VERSION     DASZYN010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915292"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VHE33_09030"
     CDS             complement(<1..772)
                     /locus_tag="VHE33_09030"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4172 domain-containing protein"
                     /protein_id="HVT97638.1"
                     /translation="MRRIKSTGYIYERKDWPQFHWDRERISALLVDVRHRQGRLIGRM
                     EGLGFQLRAEAVLHSLTEEVLKSSEIEGENLDRDQVRSSIARRLGIDIGGLTPADRDV
                     EGVVEMMLDATQKYDRPLTARRLFDWHAALFPTGRSGMTKIRVGAWRDDRNGPMQVIS
                     GPIGREHVHYEAPPAGRLRSEMKSFLDWFGKDASADLVLMAGVAHLWFVTIHPFEDGN
                     GRIARAIADMILARSEHSPQRFYSMSAQIRQERKAYYEI"
     gene            complement(926..1002)
                     /locus_tag="VHE33_09035"
     tRNA            complement(926..1002)
                     /locus_tag="VHE33_09035"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(966..968),aa:Ala,seq:cgc)
     gene            complement(1780..>2072)
                     /locus_tag="VHE33_09040"
     CDS             complement(1780..>2072)
                     /locus_tag="VHE33_09040"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HVT97639.1"
                     /translation="DPEYCRLLASRLTAAAPSTIVLFCTETTDPASIVLARSLDFNFD
                     CGRILKEALSQLGLRGGGSADFAQGEVPLAQARALRTDLIAAVRKAVADERS"
BASE COUNT          415 a          549 c          698 g          410 t
ORIGIN      
        1 ggatctcgta ataagccttg cgttcctgcc ggatctgcgc cgacatgctg tagaagcgct
       61 gcgggctgtg ctcggagcgg gcgagaatca tatccgcgat ggcgcgcgcg atgcggccat
      121 tgccgtcctc gaacggatgg atggtgacga accacaggtg cgcgaccccg gccatgagga
      181 ccaggtcggc cgaagcgtct ttcccgaacc agtccaggaa ggacttcatc tcactgcgaa
      241 gacggccggc ggggggcgcc tcgtagtgga catgctccct gccgatgggg ccggagatca
      301 cctgcatggg gccgttgcga tcgtcgcgcc aggcgcccac gcgaatcttt gtcatgccgc
      361 tgcgaccggt gggaaagagg gcggcatgcc agtcgaagag acgccgggcc gtcaaaggcc
      421 ggtcgtattt ctgagtggcg tcgagcatca tctcgacgac gccttcgacg tcacgatcgg
      481 cgggggtgag gccgccgatg tcgatgccga ggcggcgggc gatggaagaa cgtacctgat
      541 cgcggtcgag gttctcgcct tcgatctcgc ttgacttcag gacctcttcg gtaagcgagt
      601 ggagaacggc ttcggcgcgc aactggaatc caagaccctc catgcggccg atgaggcgcc
      661 cctgacggtg gcggacatcg acgagcagtg cggagatgcg ctcgcgatcc cagtgaaact
      721 gcggccagtc tttccgctcg taaatgtagc cggttgattt tattctccgc atattctgca
      781 tcgaatgtaa cgggttttct ccgcgggacg caaggctatt caccgcaata tttgcggaga
      841 ttagggcggt tattcgccgc aaaagacagg tgtactggcc gctgggttca gggcaatggg
      901 ttgggtggat ccaggcggcg ggaattggtg gagctagtcg ggctcgaacc gacgacctcc
      961 tcgttgcgaa cgaggcgctc tcccaggctg agctatagcc ccacgaaggc gggaggaact
     1021 tttgtgattt tacgtgaatc cgtggaattg cgtttttggg gggtctgaaa aatcaaaaac
     1081 aggggggtcg accccccgcc aagtcgttga tcgtaaagag aggcgctttg ggcctacccc
     1141 ctcatggata acgaagtggt taacagagga ccgcgatccg caggagtcgt gcatccggag
     1201 cgcctggaaa ttgaacttgt gtttctgcaa ctcgccgtag acgcaaataa ttgagattta
     1261 agagtttgat gcgggtttac aagtttgaca acatgctatc ttccggagga aagggcagtt
     1321 gcatcgcgat tgggaatgct gtattcgcgg ttgtgaatgc ggcaaagaca accgccaaac
     1381 aggcaaatga ttgcatcgat ggcagttgct tcgcgagcgt cggtgctgca ttcgcagttg
     1441 tcagtgcgac attcgcggtt ctcggtgctg cattcacggt tgtgagtgct gcattcgcgg
     1501 acgcgagtgc gtgatgcgca aagtttcaaa gagccagcct gatccggcct gagccggggg
     1561 cgagtacaga tcacaggtca caggtcgcgc ttgaggcgaa acctaacgct ctgcgcacga
     1621 aaccagcgtt ctgaaacacg aaagctctgc cgcctgtgcg acagagcttt ccttgtttcc
     1681 agtttgcgat gtcagccggc cggaggcaag cgaaatccga gaggcggacg gtacgaaggt
     1741 actgcggcag gttcgggcag gttccagtgc ggaacttcgt cagcttcgtt cgtcagcaac
     1801 cgcttttcgg acagcggcaa tgagatccgt ccgcagagcg cgggcttgcg cgagtgggac
     1861 ttcgccctgg gcgaaatcgg ccgagcctcc cccgcgcagg ccgagctgcg agagcgcctc
     1921 tttgagaatg cggccgcagt cgaagttgaa atcaaggctg cgcgccagga cgatggaggc
     1981 ggggtcggtg gtttcggtgc agaagagcac gatggtggat ggggcagcgg cggtgaggcg
     2041 cgaggccagc aggcgacagt actcggggtc gc
//
LOCUS       DASZYN010000352         5518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_465779, whole genome shotgun sequence.
ACCESSION   DASZYN010000352 DASZYN010000000
VERSION     DASZYN010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465779"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VHE33_09045"
     CDS             <1..649
                     /locus_tag="VHE33_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT97640.1"
                     /translation="PRRDSATNRRAIMKKIQVIVAEDQAMVLGALAALLETEPDIAVC
                     GRAANGREALTAIGKHKPDVLVTDIEMPEMTGLTLAAEVKERYPQTKVVILTTFARPG
                     YLRRALDAGAKGYLLKDRPSAELADAVRRVHQGLRVVDPDLAVEAWDSGPDPLTERER
                     QILWRAGEGKSSGEIAEELHLSEGTVRNYLSEAISKLGASNRVEAARLARARGWL"
     gene            819..1217
                     /locus_tag="VHE33_09050"
     CDS             819..1217
                     /locus_tag="VHE33_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT97641.1"
                     /translation="MATARTDRGIGTDAAATVLVVEDDRAVRLLFTKILQRAGYEVIA
                     CENGTLGFEAARKQIDKIAAIVTDSRMPGVHGPRLIARIRALRPGIPAILVSGNMVEH
                     ETDRTTVFLTKPVSPAVLTGELRRLLSAGV"
     gene            1275..2390
                     /gene="ald"
                     /locus_tag="VHE33_09055"
     CDS             1275..2390
                     /gene="ald"
                     /locus_tag="VHE33_09055"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HVT97642.1"
                     /translation="MIIGVPKEVKDHESRVGITPAGVKALSDAGHKVLVETHAGDLSA
                     FTDDEYQSAGAEIVGSAHNVWGNADMVVKVKEPVEKEYVFFRDGLVLFTYLHLAPIPD
                     LTEKLLQKKVVGIAYETIRDRAGTLPLLTPMSEVAGRMAVQVGAAYLEKEKGGRGVLL
                     GGVPGVPPAGVCVIGGGIVGTNAAKIALGMGAKVTLVDVNLNRLRELDDIFNGRLFTL
                     ASNSWNVARAVQEADLVIGGVLIPGAAAPKIVTKEMVAKMKKGAVIVDVAIDQGGCVE
                     TAHPTTHSNPAYEVNGVVHYCVTNMPAAVPNTSTLALTNATFPYVMRLAKNGAKKAIL
                     DDDGIRDGVNTYDGTLTCEPVAASQKKPWKPVRELLA"
     gene            complement(2575..3036)
                     /locus_tag="VHE33_09060"
     CDS             complement(2575..3036)
                     /locus_tag="VHE33_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97643.1"
                     /translation="MSALLVLAGLYGAGYANAQSTSRYCNDWLITGVYGFTIEGTKLA
                     GPGPTGAQVGVALTEFDGRGTLRQIDAVTIAGVQVSDFTHAVATGTYTVNADCTGTFT
                     INFTDGRPSVSTYFVVVDNGSEIDTVVEGVASGPSGMIATRSIGKRRFSFF"
     gene            3259..5220
                     /locus_tag="VHE33_09065"
     CDS             3259..5220
                     /locus_tag="VHE33_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HVT97644.1"
                     /translation="MSTPNQSERRISTGLFEVDLVSGEVLREGRVVPVQEQPFRVLRL
                     LLERPGQVVTREELQSKLWPADTYVSFDQGLNTAIRKLRVLLGDSADNPRYIETIPKR
                     GYRFIAPVRALGESKPPAQSQAQPEKAAQEGRKGVWIRAGWIAALSVLMAVGAIFATR
                     AILQRRSHGSQATPPAGRAMLVALPFQNLTDDPAQEYFSDGLTEETITDLGELSPAHL
                     GVIARTSSMAYKHTNKTIEQIGLELHANYVLEGSVRRENGRVRITAQLIRVSDQTHLW
                     AQSYDVRNLGDLIDVQSAIGRAIAEQVQVQVAPEYGADSVQLHAQNPVAYDLYLQGRF
                     YWNQRTAPALRKSIELFRQAVAADPNSPLAWAGLADAYNISNIIGALSSRQSSPQART
                     AATRALELDPSLADAHAALAMEKSHYEFDFPGARAEFQKATQLNPNSAYAHLFYSGAY
                     LMPMGLRTEAIAEMQKAVELDPLSLPINNFLGETYVLAGDYPAAYRQYQHTIAMNPNF
                     PLVHAYLADLFELTGKYEDAVHEREKTAVLSGASEQESMDKAVRLTKALKSGGAAGFW
                     KELVSQDLRARQSFAGGFDVIAQDYALAGEKDLAFQWLEKSIAAREGQELTLLAVDPM
                     WNNLHGDPRYVSLLHRIGLPEGVEVHPSN"
     gene            complement(5314..>5518)
                     /locus_tag="VHE33_09070"
     CDS             complement(5314..>5518)
                     /locus_tag="VHE33_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97645.1"
                     /translation="ALFAGGGGALGAVIGGLAGGGKGAAIGALAGAGAGTAGSAVTGN
                     KEILLPAETLVTFRLERSVHVTQ"
BASE COUNT         1104 a         1818 c         1609 g          987 t
ORIGIN      
        1 tcctcgtcga gattccgcca caaaccgtcg agccatcatg aagaagatcc aggtgatcgt
       61 cgccgaagac caggcgatgg ttctcggcgc tcttgcggcg ctgcttgaaa ccgagcccga
      121 catcgctgtc tgcggccgcg ctgccaacgg acgcgaggcc ctcacagcca tcggcaaaca
      181 caagccggac gtcctggtga ccgacatcga aatgccggaa atgaccggcc tcaccctggc
      241 ggccgaggtc aaagagcgct atccccagac gaaagttgtg atcctgacca ccttcgcccg
      301 ccccggctat cttcgccgcg ccctcgacgc gggagccaaa ggctacctcc tcaaagaccg
      361 cccctctgcc gaactcgccg acgcggtccg ccgcgtccat cagggccttc gcgtcgtcga
      421 tcccgacctc gcggtcgaag cgtgggacag cggtcccgat ccgctcaccg agcgcgaacg
      481 ccagattctg tggcgcgccg gagaagggaa gtcctcgggc gaaatcgccg aggaactgca
      541 cctctccgaa ggcaccgtgc gcaactacct ctcggaagcg atcagcaaac tcggcgcctc
      601 caaccgcgtc gaagccgccc gtctcgcccg cgcccgggga tggctctaaa gtaaccatta
      661 cttccgattc caaagggccg gaactcctcc cccttttggc gaatttcctt cgtaatttgg
      721 tcgatgctac agtcagccct tgcggtaact gcggtttccg gaatgctcgg ccctgctcgc
      781 ctcttccgat cccaggtccc ggttcgtcgg gagactcgtt ggccacggct cgcacagatc
      841 gaggcatcgg cacggacgca gcagcgaccg ttctcgtcgt tgaagacgat cgcgccgtcc
      901 gcctgctgtt caccaaaatc ctgcagcgag caggctacga agtcatcgcc tgcgagaacg
      961 gtaccctcgg cttcgaagcc gcccgcaaac agatcgataa gatcgccgcc atcgtcaccg
     1021 attcaaggat gcccggtgtc cacgggccgc gtctcatcgc ccgcattcgt gccctgcgtc
     1081 ctggcattcc cgcaatcctc gtcagcggca acatggtcga acacgaaacc gatcgcacca
     1141 cggtcttcct caccaaaccc gtctccccgg ccgttctcac cggcgagctc cggcggttgc
     1201 tctctgccgg agtgtagaat cttcagtccg gctctcactt tctcctcatc cccgaggccg
     1261 cgcaggaaga cctgatgatc attggagttc cgaaggaagt taaggaccac gagagccgcg
     1321 tgggcattac gcccgcaggc gtcaaagctc tgagcgatgc cgggcacaaa gtcctggtag
     1381 aaacccacgc gggcgatctc tccgctttta ccgacgacga gtaccagtcc gccggcgccg
     1441 agatcgtcgg ctccgcccac aacgtctggg gcaacgccga catggtcgtc aaggtcaagg
     1501 agccggtcga gaaggagtac gtcttctttc gcgatggtct cgtcctcttc acctacctgc
     1561 atctcgcacc gattccggac ctgacggaaa agcttctgca gaaaaaggtc gtcggcatcg
     1621 cctacgagac catccgcgac cgcgccggaa ccctgcccct cctcacgccc atgtccgagg
     1681 tcgctggccg catggccgtc caggtgggcg ccgcctattt ggaaaaagag aaaggcggcc
     1741 gcggcgtcct cctcggtggc gttcccggag ttccccctgc cggcgtctgc gtgatcggtg
     1801 gcggcatcgt cggcaccaac gcggcgaaga tcgccctcgg aatgggtgcc aaagtcactc
     1861 tcgtcgacgt gaacctcaac cgcctgcgcg aactcgacga catcttcaac ggcagactct
     1921 tcaccctcgc ctcgaattcc tggaatgtcg cccgcgccgt gcaggaagcc gacctcgtca
     1981 tcggtggcgt cctgattccc ggagccgccg cgcccaagat cgtcaccaaa gaaatggtcg
     2041 caaaaatgaa gaaaggcgcg gtcatcgtcg acgtcgccat cgaccagggc ggatgcgtcg
     2101 agaccgccca ccccaccacg cacagcaacc ccgcctatga ggtcaacggc gtggtccact
     2161 actgcgtgac caacatgcct gccgccgtcc ccaacacttc gacgctcgcc ctcaccaacg
     2221 ccacgttccc gtacgtgatg cgtctggcca agaacggcgc aaagaaagcc atcctcgacg
     2281 acgacggtat ccgcgacggc gtcaacacct acgacgggac gctgacctgc gaacccgtag
     2341 ccgcctcgca aaagaaaccc tggaagccgg tgagggaact gctggcttag acagcgaacc
     2401 cgcctccgat gcgattcgaa gttcgtaacg atagagcgac gcgaggaccc cagcgagcca
     2461 cgagatagat tcgctgggat ccgctccgcc agaacggttg aacaagggga acgacagccg
     2521 acaggatcga accggcgccg gatggggacg gcaagctggc tttctgtcgt cagtctagaa
     2581 aaagctgaac cggcgcttcc cgatacttct ggttgcaatc ataccgctgg ggccagaagc
     2641 aacgccctcg accacggtgt cgatctcgct cccgttgtcc accacgacaa agtaggtgga
     2701 gaccgaaggc cgtccgtcag tgaaattgat cgtgaaagtt cccgtgcagt cggcgttcac
     2761 ggtgtaggtt ccggtggcaa cagcatgcgt gaagtcggac acctggacac ctgcaatcgt
     2821 tacggcgtca atctgcctga gagttcccct gccatcgaac tccgtgagtg cgacccccac
     2881 ctgggcgccg gtggggcctg gccccgccag ctttgtgcct tcaatcgtga agccgtagac
     2941 gccggtaatg agccagtcgt tgcaatagcg cgacgtgctt tgggcgttcg cgtagccggc
     3001 tccgtaaagc ccagccagaa ccagcagcgc actcatcttt cccaatatcc tcatatgtcc
     3061 tcctcgacgg gttgaagcct tgcgacgcac cctcgtcttc cgggcgacca tcgctgcagg
     3121 cgtgtgtgct gcttataacc gaggaaagag agagggctga atgatctctg gatgacttgt
     3181 taaatcaaga ccacagaata agttacagat tcctctgaat tcgcttgtgt gctaacatac
     3241 ggttcctcga cagcgaggat gtccactccc aaccagtccg aacgcaggat cagcaccggt
     3301 ctctttgagg tggatctggt gtcgggtgaa gttctgcggg agggccgcgt ggtgcccgtt
     3361 caggagcagc cgtttcgggt cctccgtctc ctgttggagc ggcccgggca ggtggtgacc
     3421 cgcgaggagt tgcagtcgaa gctctggcca gcggacacct acgtcagctt cgatcaggga
     3481 ttgaacacgg ccattcgcaa gctgcgcgtg cttcttggcg actccgcgga caatccccgg
     3541 tacattgaga cgattcccaa acgggggtac cgcttcatcg ccccggttcg agcgctggga
     3601 gagagcaaac ccccggcgca gagccaggcg caaccggaga aggcggctca ggaaggaagg
     3661 aagggggtat ggatccgggc cggctggatc gcggctctct cggtgctgat ggcggtgggg
     3721 gcgatcttcg caacccgggc catcctgcaa cgtcgttccc atggttctca ggcgacgcct
     3781 cccgcggggc gagccatgct ggtggctctg ccgtttcaga atctgactga cgacccagcc
     3841 caggaatact tcagcgacgg gctgaccgag gagacgatca ccgatcttgg cgagttgagc
     3901 cccgcgcatc tgggagtgat cgcccgaacc tcatcgatgg cctacaagca cacgaacaag
     3961 accatcgaac agatcggcct ggaactgcat gccaactacg ttctcgaagg cagtgtgcgg
     4021 cgagaaaacg ggcgggtgcg catcactgca caactgatcc gtgtgagcga tcagacgcat
     4081 ctttgggcgc aatcctacga cgtgagaaac ctgggcgacc tgatcgacgt tcagagcgcc
     4141 attggaagag ccattgccga gcaggtccag gtgcaggtcg ctccggagta cggggcagac
     4201 agcgtgcagc tgcatgcgca gaatcctgtg gcttacgatc tttacctgca ggggcgcttc
     4261 tactggaacc agaggacggc gccggcgctt cgcaaaagca tcgaactctt caggcaggcc
     4321 gtcgccgcgg atccgaattc gcctctggcc tgggccggcc ttgccgacgc ttacaacatc
     4381 agcaacatta ttggcgcgct gtcttcccgg cagagttctc cccaggcccg gacggccgcc
     4441 accagggccc tcgaactgga tccttcgctg gccgacgccc acgcggcgct ggccatggag
     4501 aagtctcact acgaattcga ttttccggga gccagggcgg agtttcagaa ggccactcag
     4561 ctcaatccga attcggccta cgcccatctg ttttattcgg gggcctacct gatgcccatg
     4621 ggcctgcgga ccgaggccat tgccgagatg cagaaagcgg tcgagctgga tcctctgtct
     4681 ctgcccatca ataacttttt gggcgagacc tatgtgctgg ccggggatta tccagccgct
     4741 tatcggcagt atcagcacac aatcgcgatg aacccgaact tccccctcgt ccacgcgtac
     4801 ctggcggatc tcttcgagtt gacgggcaag tacgaggacg cggttcacga gcgcgaaaag
     4861 accgcagttc tttccggcgc aagcgaacag gaatccatgg acaaggcagt tcgtttaacg
     4921 aaggcactga agagcggcgg tgcagcgggt ttctggaagg agttggtgtc gcaggatctt
     4981 cgcgcccggc agagcttcgc cggcggcttt gacgtcatcg cccaggatta cgcactggcc
     5041 ggggaaaagg acctggcatt ccagtggctg gaaaaatcga tcgcggcacg ggaaggtcag
     5101 gagctgaccc tgctcgcggt tgacccgatg tggaacaacc tccacggcga tccccgatat
     5161 gtgagccttc tgcatcgcat cggcttaccg gaaggggtcg aggttcaccc ttcgaactga
     5221 tcggcggtcc tcgtcgaagt gggatttccc tggagcactc ccgaaaacaa aaaggacgcg
     5281 gccaaggccg cgtccctttt ggtacctcgc tccttactgc gtcacatgca ccgagcgctc
     5341 cagccggaac gtcaccagcg tctctgccgg caacagaatc tccttgttcc cggtcaccgc
     5401 actccccgcc gttccagcgc ccgcaccggc cagcgcgcca atcgccgcgc ccttgccgcc
     5461 accggccagc ccgccgatga ccgccccgag tgctccgccg ccacccgcaa acaatgcg
//
LOCUS       DASZYN010000353         9196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_16513, whole genome shotgun sequence.
ACCESSION   DASZYN010000353 DASZYN010000000
VERSION     DASZYN010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 9196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9196
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16513"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2315)
                     /locus_tag="VHE33_09075"
     CDS             complement(<1..2315)
                     /locus_tag="VHE33_09075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT97646.1"
                     /translation="MSVDLKTVLRQLRKSPGFTITAVLTLALGIGATTAIFTLVDQVL
                     LKSLPVKDPGQLVRVGDNEQCCFNGGIPTYTGKPNDWSLFSYPQYAEFRDHTPGLQSL
                     AAFEGGDRELAVRRAGSRDTARPFYGEFVSGNSFDVLGLRAYAGRMLEPSDDMTSAAP
                     VAVMSFQMWEQQLGRDPSVVGSSWQINGQTVTVVGIAPPGFYGERLSDTPPSFWMPLH
                     LVAAVWPRDADLLERGEQQWLNLMGRLAPGASAAAVQARMVVELQAFLRSPVSKLQAA
                     DQPLIPQQYLRLSPGGGGVQRMQEQYRGDLHLLMWVSSFVLLIACANLANLMLTRSVT
                     QRQQTAVRAALGAQRKILVRRALLECLLLAMGGGLAGIVVAWGGAKLILHLAFAQDPI
                     TISPSPSLVVLGFAFVASLLTGLLFGVAPAWKAAQADPIEALRGANRATGRHETFAQK
                     ALVVAQAAVSVVLLCAAGLLILSLRKLEHQHFGFETKNRTIAMLNVQTAGLAPEQLDD
                     FYRKLGQSLSEIPGVERVAWSTWSPMDGNNYSETTYIEGQQPPPPGSAVNTTSWLRIS
                     PDYFATIGTKLVEGREFAESDNRAGQPVAIVDRNFVKQFLHGAHAIGAHFGDFDAAHP
                     AIYTIVGVVENAQYWPPNDPQEQGHPMYFLPAAQWVAPPPASPQAVSYARFLTNTHYM
                     NSLEIETHGRVPELEAEVRNRVAEIDPRLMVVHYHTFAEQVQQAFSQQQMIVRLTSLF
                     GLVALALAAIGLYGVTAYAVAQRTGEIGIRMA"
     gene            2631..4592
                     /locus_tag="VHE33_09080"
     CDS             2631..4592
                     /locus_tag="VHE33_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97647.1"
                     /translation="MQRFVSIPCFLLFATSLAAAQSTMAPLLTRAWDNHRSGWNQHET
                     VLTQANVKAKGITRITTIPVYGDARGIESQPLILPGVQLADGTTHDVMVLPSMANIVR
                     GVDATTGAALWQTPQLGTPITGSDSLGPNGPGAMCPGPNQTIDCYQINDKWGVLSTGV
                     IDPDTQLVYLVAWISPDTTPQNGKHFMYVLNVKDGSQAVAPVLLDGKSGTQTWSQFMR
                     KQRSSLVMTNIAGRKTIFFASGTIQELGTGAAGWVLAYDVATNQVTAALATSQGFGGG
                     IWMGGQGLAADSQGFLYGVTGNGSFDGVNDFAESILKIQYTPPAGQTPASLKIVSWWT
                     PYSDTARSPGGYPGPTAPVAAKPAGVSAPSQAVEPVNAAMRPSIKHARIVKKKNRRGQ
                     TVPLVYPKIANTPGAWWDEDLGSAQGALLENEGVYIVTGKDGIAYPTRIANLGNTMPA
                     DLANAHANCAKLASPPVWLTEDPGPVDPCPADPATLNFMPWGKTRHMHMTPVQYRSPT
                     RGQVLFAWGENSQLHAWAVSPTGALTYLAQGNEFASPNVANTPGGMPGGFCSMSSNGS
                     TPGTALLVCTMPYGDANTTVTNGRLLVYDPDNWTTNPDGSLTIPVLWDSQQWNIQFLY
                     NKFNPPIVDGGEIYVPNYNGGVDVYSLTP"
     gene            4672..6666
                     /locus_tag="VHE33_09085"
     CDS             4672..6666
                     /locus_tag="VHE33_09085"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HVT97648.1"
                     /translation="MTAFTWFAARLRLTLASAAPKLCMRRHAIACLATFFTFYPACSR
                     APAQTSPVAWPNGSVDINSGWRAQDGDNLAWAQPGFDDSSWPIITLNAANNTAGWRWY
                     RLGTNLPATPTPLALLITAGRGTFEVYLNGQRLPGPGIQSDLLITYPRSQVVYFPPSS
                     GAATIALRTWIPPTSMFFADRGALRVLLGTQSAIYDAHRAETSTRFDKLVAGLAIYLL
                     VLFAGLSLLLLFWFQPHHREYLWLGIYFLLGDGLNAVCFILSSYGFTPFCLVWFVSTP
                     ANYLQLIAMIEFTFSFVGQRVTRPVRAYEALLVLWPLCFLLPAWFGVITRNFYNIVEA
                     GLFLPASIFVPVLLLSWYRRGNREAGWLIFPSPLPLLTLCLSDVGVLGESMGSRRLAI
                     FDQQLPVGGFAISFFEAADLLFLLAIGVVMFFRFTRVSREQARSAAELQAAREIQQRL
                     VPVSLPRIAGYALQAAYLPAQEVGGDFYQVLEQRDGATLIVIGDVSGKGLKAAMTGTL
                     AIGALRSLADEDLGPAVLLNRLNREMLRAQDGGFITCLCARLATNATVTVANAGHLPP
                     FRNGDEITLESALPLGLTSEAVFPETTLCLAPGDSLTFLSDGVVEARNPTGELYGFDR
                     TRAISTQSAQQIARAAQQFGQEDDITVLTLTFTGAEVPHA"
     gene            6659..8647
                     /locus_tag="VHE33_09090"
     CDS             6659..8647
                     /locus_tag="VHE33_09090"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HVT97649.1"
                     /translation="MRRLVLLAIPLLVIAALALPARAQTFDMERGRMQMTLLNGLMRF
                     HTGDDLRWSQAAFDDSAWPLISSQKDWSAQGYKDYGGFAWYRFKVLLPNQHAQLALYF
                     PPIRTSYQVFADGTLVGSFGGFPPHGSIDTSLPQLVLLPQTKAGEMEIALRVWHWPHW
                     AMYFGGGLSSAPRIGDAGLLREWTTLQDRNTSWHLTAQGYLALLNFLYAIAGFALFLM
                     RRRERLYLYYALAGLFFGLWSLANIYAAFHSVPALASQVLMNGLSVAGFISFMIFVWM
                     MLGARRTIWIPLCLVSAALDVVMWAVPALRDLPVAAGNFIFLVVNLPLTLAPVAMLVQ
                     GARRRNPDARLLLVPVGLNTLANIINDTLWAMLTTGHSWVAPLWTFWNQTFSWPFPFG
                     LYELSIAILLIAILAIVVLRFARSRHEEDQLRSEREAARAVQQVLIPDAIPEIPNFRI
                     ESVYSPAGEVGGDFFQILPTPAGGVLLAIGDVSGKGMPAAMTVSLLVGTIRTLAHYTQ
                     SPAEILFAMNQRMLGRTQGGFTTCLVLRADPDGAVTTANAGHLAPYLGGTELCIDNGL
                     PLGLSAEGTYPEARLTLPGNTQLTLLTDGVVEARNAQGELYGFDRTASISAQSAGQVA
                     AAAQAHGQEDDITVLTLTRLTAGERPTMESIAPVLAPA"
     gene            8982..>9196
                     /locus_tag="VHE33_09095"
     CDS             8982..>9196
                     /locus_tag="VHE33_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97650.1"
                     /translation="MNILKKVGVKVADFGKWLAGEIKTIVGLAIRVEHILKSEKPLEK
                     PFIQGLSIVVADVEALIAASETALNANG"
BASE COUNT         1638 a         3515 c         2302 g         1741 t
ORIGIN      
        1 gccatgcgaa tcccgatctc gcccgtcctc tgtgccaccg catacgccgt cactccatac
       61 agcccgatcg ccgccagcgc cagggcgacc aacccgaaca gcgacgtcag ccgcacaatc
      121 atctgctgct ggctgaacgc ctgctgcacc tgctctgcga aggtgtggta gtgcaccacc
      181 atcagccggg gatcgatctc cgccacccgg ttccgcactt cggcctccag ctccggcact
      241 cgtccgtgcg tttcgatctc cagcgagttc atatagtgcg tgttggtcag gaaccgcgca
      301 tagctcaccg cttgcggcga cgccggcggc ggagcgaccc actgcgcagc gggcaggaag
      361 tacatcggat gcccctgctc ctgcggatcg ttcggcggcc agtactgcgc gttctccacg
      421 acgccaacaa tggtgtagat cgccgggtgg gcggcgtcga aatctccaaa atgcgcgccg
      481 atcgcatgtg ctccgtggag gaactgcttc acaaaattcc gatccacaat cgccaccggc
      541 tgcccggccc gattgtcgct ctcggcgaat tcgcgcccct ccaccagctt tgtcccaatc
      601 gtcgcaaaat aatccgggct gatgcgaagc cacgacgtcg tattcaccgc cgagcctggt
      661 ggcggcggct gctgcccctc aatgtacgtg gtctcgctgt agttgttccc gtccatcggg
      721 ctccacgtcg accacgccac tcgttcgaca ccgggaatct ccgagagcga ctgtccgagc
      781 ttccggtaga aatcgtccag ttgctccggc gccagtcccg ccgtctgtac gttcagcatc
      841 gcgatcgtcc ggttcttcgt ctcgaatccg aaatgctgat gctccagctt gcgcaggctc
      901 aggatcagca ggcccgccgc gcacagcagc accactgaaa cggcagcctg cgccaccacc
      961 agcgccttct gcgcgaatgt ctcgtgtcgc cccgtcgccc ggttcgcccc gcgcagcgcc
     1021 tcaatcggat ccgcctgcgc cgctttccac gctggcgcca ctccaaacag cagcccggtc
     1081 agcagcgacg ccacaaacgc aaaccccaaa accaccagcg acggactcgg gctgatcgta
     1141 atcggatcct gggcgaacgc aaggtgcaga atcagcttcg caccacccca tgccaccaca
     1201 atgccggcca ggccgccccc catcgccagc agcagacact caagcagggc acgccgaacc
     1261 agtatcttcc gctgcgcgcc caacgccgcc cgcaccgcgg tctgctgccg ctgggtcacg
     1321 ctccgtgtca gcatcagatt cgccagattt gcgcacgcaa tcagcaaaac gaaactcgac
     1381 acccacatca gcaaatgcag atcgccgcga tactgctcct gcatccgctg cacgcccccg
     1441 ccccctggcg agagtcgcag atattgctgc gggatcagag gctggtcggc tgcctgcagc
     1501 ttgctcaccg gacttcgcag gaacgcctgc aattccacca ccatgcgcgc ctgcaccgca
     1561 gccgcactgg cccccggcgc caggcgtccc atcaggttca gccactgctg ctcgcctcgc
     1621 tccagcagat ccgcgtctcg cggccacacc gctgccacca aatgcaatgg catccagaac
     1681 gacggcggcg tatcactcag tcgttcgccg tagaaccccg gcggagcgat cccaaccacc
     1741 gtcaccgttt gcccgttgat ctgccagctc gaccccacga cgctgggatc gcgccccagt
     1801 tgctgctccc acatctgaaa gctcatcact gccaccggag ccgcactcgt catatcgtcg
     1861 ctcggctcca gcatccgccc tgcatacgcc ctcagtccca gcacatcgaa ggaattgccg
     1921 gaaacaaact ctccgtagaa cggccgcgcc gtgtcgcgac tgcctgcacg cctcaccgcc
     1981 agttcccgat caccgccttc gaacgccgcc aggctctgca gccccggcgt gtgatcgcga
     2041 aattcggcgt actgcggata agaaaacagc gaccagtcgt tcggcttgcc cgtatacgtc
     2101 ggaataccgc cgttgaagca gcactgctcg ttgtccccca cccgcaccag ctgccccgga
     2161 tccttcaccg gcagcgactt cagcagaacc tgatccacca gcgtgaagat cgccgttgtc
     2221 gcaccgatgc ccagcgccag cgtcaacacg gccgtgatcg taaacccagg cgacttccgc
     2281 agctggcgca ggacggtttt taaatcaaca gacatgctcg agcatctcct cgggccgttt
     2341 tgagaccccg tccggttgga ttcggttacg accctccgtg ttttgtctgc aagtcgcgtt
     2401 ccatcgtctc acgacataat ctattgaaag caaacagtcg ctcctcagcc aacgcacggc
     2461 gatcgtccag cctccgattg cgctgttcgc cagcggacag gttttgcttt ccccgcgaat
     2521 tcgtggcacg ataagtccca cttcgctccc cagcgcccaa aaccagcaca aaagacagcc
     2581 gtccagaaac ttcagcgcag gatcagccac caaccccacg ggaggatccg atgcagcgtt
     2641 tcgtctccat tccctgcttc cttctcttcg caacatccct cgccgccgct cagtccacca
     2701 tggcccccct cctcacgcgt gcctgggaca atcaccgctc cggctggaac cagcacgaga
     2761 ccgtcctcac ccaggccaat gtcaaagcca aaggcatcac ccgcatcacc accatccccg
     2821 tctatggcga tgcgcgcggc atcgaatcgc agcccctcat tcttcccggc gtgcaacttg
     2881 ccgacggcac aacccacgac gtcatggttc tcccgtcgat ggcgaacatc gttcgcggcg
     2941 tcgacgccac caccggcgcg gcgctctggc agacgccgca gcttggcacg ccgatcaccg
     3001 gcagcgactc gctcggcccc aacggccccg gtgccatgtg ccctggtccc aaccaaacca
     3061 tcgactgcta tcagatcaac gacaagtggg gcgtgctctc caccggcgtc atcgatcccg
     3121 atacgcagct cgtctacctc gttgcctgga tttcccccga caccacgccg caaaacggca
     3181 agcactttat gtatgtcctg aatgtgaagg acggctctca ggccgtcgcg ccggttcttc
     3241 tcgacggcaa aagcggcacg caaacctggt cgcagttcat gcgcaaacag cgcagctcgc
     3301 tcgtcatgac caacatcgcc ggccgcaaaa cgatcttctt cgcttccgga accattcagg
     3361 aactcggcac cggcgccgcc ggctgggtcc tcgcctacga tgtcgccacg aaccaggtca
     3421 ccgcggccct cgccacttcg cagggctttg gcggcggcat ctggatgggc ggccagggac
     3481 tcgccgccga ttcccagggc ttcctctacg gcgtcaccgg caacggctcc ttcgatggcg
     3541 tgaacgactt cgcggaatcc atcctgaaga tccagtacac gccgcccgcg ggccaaactc
     3601 ccgcctcgct caaaatcgtc agctggtgga ctccctacag cgacacggct cgctcccccg
     3661 gcggataccc cggccccacc gcaccggtag ccgcaaagcc cgccggagtc agcgccccct
     3721 cccaagccgt ggaacccgtc aacgcggcca tgcgcccctc catcaaacac gcccgcatcg
     3781 tcaaaaagaa gaaccggcgc ggccagaccg ttcccctcgt ctatccgaag atcgccaaca
     3841 cccccggtgc ctggtgggac gaagacctgg gctcagcgca gggcgccctg ctcgaaaacg
     3901 aaggcgtcta catcgtcacc ggcaaagatg gcatcgccta ccccacccgc atcgccaacc
     3961 tcggcaacac catgcccgcc gacctcgcca acgcacacgc caactgcgcc aaactcgctt
     4021 cgccgccggt gtggctcacc gaggatcccg gccccgtcga cccctgcccc gccgaccccg
     4081 ccaccctcaa cttcatgcca tggggcaaga ctcgccacat gcatatgact cctgtccagt
     4141 acaggagccc cactcgaggc caggttcttt tcgcgtgggg agaaaacagc cagctccacg
     4201 cctgggcggt ttcgcccacc ggcgcgctca cctacctcgc ccagggcaac gagtttgcct
     4261 cacccaacgt cgccaataca cccggcggaa tgcccggcgg cttctgctcc atgtccagca
     4321 acggctccac gcccggcacc gccctgctgg tctgcaccat gccctacggc gatgccaata
     4381 ccaccgtcac caacggccgc ctgctcgtct acgatcccga caactggacc accaatcccg
     4441 acggcagcct caccatcccc gtcctctggg attcgcagca gtggaacatc cagttcctct
     4501 acaacaaatt caatccgccc atcgtcgacg gcggcgagat ctacgtcccc aactacaacg
     4561 gcggggtcga tgtttacagc ctcacgccct gatccgagtt cctcaggccc ctgcctcttc
     4621 tcgacgccac cctgtactat gtccctcatc ccccccggta acaagatccg gatgaccgcc
     4681 ttcacctggt ttgccgcgcg gctgcgcctc accctcgctt cagcagcgcc aaagctctgc
     4741 atgcgcagac acgccatcgc ttgtctcgca acgtttttca ctttttaccc cgcatgttcg
     4801 cgtgcccccg cacaaacgtc tcctgtagcc tggcccaatg gctcggtcga tatcaacagc
     4861 ggctggcgcg cccaggatgg cgacaacctc gcctgggctc agcccggctt cgacgacagc
     4921 tcctggccca tcatcacgct gaacgccgcc aacaacaccg ccggctggcg ctggtatcgc
     4981 cttggcacca acctccctgc cacacccacc ccgctcgccc tgctcatcac ggctggccgc
     5041 ggcactttcg aggtttacct caacggccag cgtctccctg ggcccggcat ccagtccgac
     5101 ctgctcatca cctacccccg ttcccaggtc gtctattttc cgccatcgtc cggcgccgca
     5161 accatcgcgc tccgcacctg gatcccccct accagcatgt tcttcgccga tcgcggcgcc
     5221 ctccgcgtct tgctgggaac ccaatccgcg atctacgacg cccatcgcgc cgaaaccagc
     5281 actcgattcg acaagcttgt cgccggcctc gccatctatc tcctggttct tttcgcagga
     5341 ttgtcgctgc tcctcctctt ctggtttcag ccccatcacc gtgagtacct ctggcttggc
     5401 atctacttcc tcctcggtga cggtctcaac gcagtctgct tcatcctctc cagctatggc
     5461 ttcactccct tttgcctcgt ctggtttgtc tcgaccccgg caaactatct ccagctcatt
     5521 gcaatgatcg aattcacctt cagcttcgtc ggccagcgtg tcacgcgccc tgtccgtgcc
     5581 tacgaggcgc tcctcgtgct ctggccgctc tgctttctcc ttcccgcctg gttcggagtc
     5641 atcacccgaa acttctataa cattgtcgaa gccggactct tcctgccggc ctccattttc
     5701 gtgcccgttc tcctgctctc ctggtatcgc cgcggcaatc gcgaagccgg atggctcatt
     5761 ttccccagtc ctcttcctct gctcaccctt tgcctcagcg atgtcggtgt tctcggcgaa
     5821 tccatgggct ctcgccgcct cgccatcttc gaccagcagc tgcctgtggg cggtttcgcc
     5881 atctcgtttt tcgaagccgc tgacctgctc ttcctcctcg ccatcggcgt ggtcatgttc
     5941 ttccgcttca cccgcgtcag ccgcgaacag gcccgctccg ccgccgagct ccaggcggca
     6001 cgcgaaatcc agcaacgcct cgtccccgta tcgctgccac ggatcgccgg ctacgcgctc
     6061 caggccgcct atctccccgc gcaggaagtg ggcggcgact tctatcaggt gctggaacaa
     6121 cgcgacggcg caaccctgat cgtcatcggc gacgtcagcg gcaaaggcct caaggccgcc
     6181 atgaccggta ccctcgccat cggcgccctg cgttccctcg ccgatgaaga cctcgggccc
     6241 gccgttcttc tcaaccgtct caaccgtgaa atgctgcgcg ctcaggacgg cggctttatc
     6301 acttgcctgt gcgcgcgcct cgcaacaaac gccactgtta cggtcgccaa tgcgggccac
     6361 cttcctccct ttcgcaacgg tgatgaaata acactggaat ccgcccttcc gctcggcctc
     6421 acctcggagg cggtcttccc cgagaccacc ctttgcctcg ctcccggcga ttccctcacc
     6481 tttctctccg acggtgtcgt tgaagcccgc aaccccaccg gcgagctcta cggcttcgac
     6541 cgcacgcgtg ccatctccac ccagtccgcc cagcagatcg cccgcgctgc ccagcagttc
     6601 ggccaggagg acgacatcac cgtcctcacc ctcaccttca ccggagccga ggtcccgcat
     6661 gcgtagactc gttctgcttg ccatccccct ccttgtgatc gctgcgctgg ccctgcctgc
     6721 ccgcgcgcag accttcgaca tggagcgcgg ccgcatgcaa atgaccctgc tcaacggcct
     6781 catgcgcttc cataccggcg acgatttacg ctggtcccag gccgccttcg acgactccgc
     6841 ctggcctctc atctcctcac aaaaagattg gagcgctcag ggctacaagg actacggcgg
     6901 cttcgcctgg taccgcttca aagtcctcct cccgaaccag cacgcccagc tcgcccttta
     6961 cttccccccc atacgcacca gttaccaggt ctttgccgat ggcaccctgg tcggctcctt
     7021 cggcggcttt ccaccgcacg gcagcatcga cacctccctc ccgcagctgg tgctgctgcc
     7081 tcagacgaag gccggcgaga tggagattgc cctccgcgtt tggcactggc cgcactgggc
     7141 catgtacttc ggcggcggcc tgtccagcgc gccacgcatc ggcgatgccg gcctgctcag
     7201 ggaatggacc accctccagg accgaaacac ctcctggcat ctcaccgcgc agggctacct
     7261 cgccctcctc aatttcctct acgccatcgc cggctttgcg ctcttcctca tgcggcgcag
     7321 agaacgtctg tacctttact acgccctggc cggtctgttc ttcggtctct ggtccctggc
     7381 gaacatatac gctgcctttc actctgtgcc cgcgctcgcc agccaggttc tgatgaatgg
     7441 cctttccgtt gccggtttca tcagcttcat gatctttgtc tggatgatgc tcggcgcccg
     7501 gcgcacgata tggatcccgc tgtgcctcgt ctccgccgcg ctcgacgtgg tgatgtgggc
     7561 ggtgcccgcg ctccgcgatc tgcccgttgc agccggcaac ttcatctttc tcgtcgtcaa
     7621 tttgcccctg acactcgccc ctgtcgccat gttggtccag ggcgcccggc gccgcaatcc
     7681 tgatgctcga ttactgctcg ttcccgtggg attgaacacc ctggcgaaca tcatcaatga
     7741 caccctctgg gccatgctga ctaccggtca ttcgtgggtt gcgccgttgt ggaccttctg
     7801 gaaccaaacc ttcagctggc cctttccctt tggcctgtac gagctttcga tcgcgattct
     7861 gctgatcgcg attctcgcca tcgtcgtcct tcgctttgct cgatcccgcc acgaggaaga
     7921 ccagctccgc agcgaacgcg aagcagcacg ggctgtgcag caagtcctca tccccgatgc
     7981 aatccccgag ataccaaact tccgcatcga gagcgtttac tcgcccgctg gcgaggtcgg
     8041 cggcgacttc ttccagattc tccccacacc cgcaggtgga gtcttgctgg ccatcggcga
     8101 cgtcagcggc aaaggcatgc ccgccgccat gaccgtctcc ctgctcgttg gtaccatccg
     8161 cacgctcgcc cactacacac aaagtcccgc ggaaatcctc tttgcaatga accagcgcat
     8221 gctcggccgc actcagggcg gattcaccac ctgcctggtc ctgcgtgccg accccgacgg
     8281 cgccgtcacc acggccaacg ccggccatct cgctccttac ctgggcggca cggagctctg
     8341 tatcgacaat ggactgcccc tcggcctcag cgccgaaggc acgtatccgg aggccaggtt
     8401 gaccctcccc ggcaacacgc aactcaccct cctgaccgat ggcgttgtcg aggcccgcaa
     8461 tgcgcagggc gaactctacg gcttcgaccg caccgcatcc atatccgccc agtccgccgg
     8521 ccaggtcgcc gcggccgccc aggcccacgg tcaggaagac gacatcaccg tcctcaccct
     8581 aacccgcctt accgccggcg aaagacccac catggagtca atcgcgccgg tcctggcccc
     8641 agcataagag gcggccgatc ctctcctcgc gccacggttt ttccccccac gtcgtcgccg
     8701 aacccttctt tccaggatcg cctcatcgct gacccctgat tggctcgaaa acacgtccca
     8761 gcgcgttttc accccaaatg tgcaaaactt gtcaagcttt tcgcctcatt acgaaagtgc
     8821 atgtctctga aaaagcatga cttccacgcc aaaaacgttg gcacgattgg cctcgccgct
     8881 tggtatcctg aaaatagagg ggaattctca aaaagaggcc cgcctaacca ggcgggcctt
     8941 ttcttttctc ctcgttttgc atgcaaagga aagccaacaa catgaatatt ctcaagaaag
     9001 tcggagtcaa agtcgcggac ttcggcaagt ggctcgctgg cgagatcaaa accatcgtcg
     9061 gcctcgccat ccgcgtggag cacatcctca agtccgagaa gccactggag aaacccttca
     9121 tccagggcct ctccatcgtg gtcgccgacg tcgaggccct catcgcggcc tccgaaacgg
     9181 ccctgaacgc caacgg
//
LOCUS       DASZYN010000354         1790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1235289, whole genome shotgun sequence.
ACCESSION   DASZYN010000354 DASZYN010000000
VERSION     DASZYN010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235289"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            639..1133
                     /locus_tag="VHE33_09100"
     CDS             639..1133
                     /locus_tag="VHE33_09100"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HVT97651.1"
                     /translation="MTIAELFLEEFDLEMPGTRKLLERVPDDRFAWKPHEKSMTLGRL
                     SSHIADLPARCSTIITTEELVRQPGFTPWAAASTVDLLDHFDSAATQARAALTGLRED
                     QLSATWTIKMGDRTLASMPRVMALRRVFLDHIIHHRAQLGVFLRLLDIPIPGVYGASA
                     DDQR"
     gene            1228..>1790
                     /locus_tag="VHE33_09105"
     CDS             1228..>1790
                     /locus_tag="VHE33_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HVT97652.1"
                     /translation="MTGRVFSKLLLSFVLVLCIATAILDLSFRNIVEHSLHEEARVAS
                     LSVEQRAAALHILRRDLFSSSLIALVLATLVSAWLAHRIAARLRRIVDFSGRIAGGDF
                     TARIEEDAEDEIGEVARALDTTAGRLQQSFHALEAKQRELAALLDSMQEAVIAVDARG
                     QVSWSNAIMQRISTAPVRPGKALVVSVR"
BASE COUNT          343 a          678 c          440 g          329 t
ORIGIN      
        1 acagcaagaa gcccatactc ccggcgtgcg aaatgcaagg aatatttccc cgcttagact
       61 tctccggtac cgccctaatc ccattttgga acgcgccaca ttcaccgacc accgaggagc
      121 gcgaacgcca ggttcacccc acagcaagaa gcccatactc ccggcgtacg aattgcaagg
      181 aatatttccc cgctcagact tctccggtac cgccctatcc cattttggaa cgcgccacat
      241 tcaccgacca ccgacgagcg cgaacgccag gttcacccca tagcaagaag cccatactcc
      301 cggcgtacga attgcaaggg atatcgcccg gcgcagaatt cctcggtacc gacccgctcc
      361 cattttggaa ctccgcgcac acaccccacc cccgacgagc gcgcacgcca ggttcacccc
      421 acagcaagaa gccaagacct ttccaatacg gattgcaacc acttttgctc tccgaacggt
      481 tccacaagta ccgcccgatt cccattttga aaatatccag tccgtatctg cttccatcag
      541 accgcgtcgg gtaccctttc cccggacgag gcgccaggcc cccacacccc ttgcgacagt
      601 tggttgtaaa gtgaagccaa acccaggatc aacgacccat gaccatcgct gaactgtttc
      661 tcgaagagtt cgatctggag atgcccggca cccgcaagct cctcgaacgc gtgcccgacg
      721 atcgcttcgc ctggaaaccg cacgaaaaat cgatgaccct gggccggctc tcctcccaca
      781 tcgccgacct gcccgcgcgc tgctccacca tcatcacgac cgaggagctg gtccgccagc
      841 caggcttcac tccctgggct gctgcatcga ccgtcgatct cctcgaccac ttcgattccg
      901 cggcgaccca ggcccgtgcc gcgttgaccg gccttcgcga ggatcagctt tccgccacct
      961 ggaccatcaa aatgggcgac cgcacccttg cttccatgcc gcgcgtgatg gcattgcgcc
     1021 gcgtcttttt ggatcacatc atccatcacc gcgcccagct cggcgttttc ctgcgtctgc
     1081 tcgatattcc aatccctggc gtctacggtg cgtccgccga cgatcagcgc tgaagggacg
     1141 cctcacccgg cctccgccaa cggctgtccg cagggcgaca ggctcaactg aacgctgtac
     1201 gctgaacgct gtacgctgca tttcctcgtg accggcagag tcttctccaa actgctcctc
     1261 tccttcgtcc tcgtgctgtg catcgccacg gccatcctgg acctgtcttt ccgcaacatc
     1321 gtggagcact ccctgcacga ggaagcgcga gtcgcctcac tcagcgtcga acagcgtgcc
     1381 gccgccctgc acatcctccg ccgcgatctc ttcagttcgt cgctcatcgc gctcgtcctc
     1441 gccaccctcg tttcagcctg gctcgcccat cgcatcgccg cgcgcctgcg ccgcattgtc
     1501 gatttctccg gccgcatcgc cggcggggac ttcaccgcgc gcatcgagga ggacgctgag
     1561 gacgaaatcg gtgaagtggc acgcgccctc gacaccactg cgggccgcct gcagcagtcc
     1621 ttccacgccc tcgaagccaa acagcgcgag ctcgctgccc tcctcgactc catgcaggaa
     1681 gccgtcatcg ccgtggacgc caggggccag gtgagctggt ccaacgccat catgcagcgc
     1741 atctcgaccg ccccggtaag acccggcaaa gcgctcgtcg tcagcgttcg
//
LOCUS       DASZYN010000355         3181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_143961, whole genome shotgun sequence.
ACCESSION   DASZYN010000355 DASZYN010000000
VERSION     DASZYN010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143961"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VHE33_09110"
     CDS             complement(<1..596)
                     /locus_tag="VHE33_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HVT97653.1"
                     /translation="MPILLDGKAYSKELEAELTLRVQRLHLEQPPILATILVGDDPAS
                     ATYVKMKGNACRRVGMESLSIHLAGTTTTAELLGAIDELNGNPRVRGILLQHPVPKQI
                     DERACFDRIALDKDVDGVTAHGFGRMAMDEPAYGSATPAGIMRLLRRYQIPIEGREAV
                     VVGRSPILGRPMAMMLLAANATVTICHSRTKNLADVVSR"
     gene            complement(682..1968)
                     /locus_tag="VHE33_09115"
     CDS             complement(682..1968)
                     /locus_tag="VHE33_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97654.1"
                     /translation="MRFQRLLTSSYAALVLAALLSGCGTTTSNGGTPQSPPPTGNPGG
                     GGGTTSPAPSVAAYVYVVNSADYTPSSPRQIVAYAADMNGQLRPVSGSPFNENVNSVA
                     TNGSYLMAAAVATPNINTYSIGPNGVPTRTQQFDYSQQTQYQANACGIVGDLEFDHTG
                     QSLYAGVGQYGCSTNNGIASFSVDPTAGSLTYLGIQNIGYNTSPRVHFDGSNTYAYSA
                     YNDGCYIFSLTAMIRDSNGLLAENSTYTAPPLLTPPPGAQETMVQAFYPGPSASDSTD
                     HFVFAEFPCYKNSGTPAQLATYTIDASGNLSTTDTYATMPTTKVSAPEDLKMSPSGTL
                     LAVGGKGGLEIFHFDGTALTADGDLLTTDPIAQMFWDSHNHLYAITDNDTGTGGISPG
                     KLYVFTVQDSQISAPEQAPGSPYPLATPWYLAVQSK"
     gene            complement(2278..2817)
                     /locus_tag="VHE33_09120"
     CDS             complement(2278..2817)
                     /locus_tag="VHE33_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97655.1"
                     /translation="MNIEVASCPSRILTSWKEIAQYLNKGVRTVQRWEQDFGLPVRRP
                     HGANKKAIIARTSDIDLWLATRCTTRISASAKIELGDTLRALCAELGSQIAMSAMLHS
                     SEPTGNDILATVAALRQEMTRLALLLTIPASSIFQQTPSLPNGLPRPELVEPQKCVPL
                     ESPGVRQESEAPISLPLAI"
     gene            complement(2970..3161)
                     /locus_tag="VHE33_09125"
     CDS             complement(2970..3161)
                     /locus_tag="VHE33_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97656.1"
                     /translation="MIKFLLFCILLFLCWPLALLALIAYPIVWLLLIPFRLLGVAVHG
                     VLDLVGAVITLPVRLIRAI"
BASE COUNT          633 a          870 c         1055 g          623 t
ORIGIN      
        1 cggctcacga cgtccgccag gtttttagtg cgcgagtggc agatggtcac cgtggcgttg
       61 gcggcgagca gcatcatggc catcgggcgt ccgaggatcg ggctccgccc aaccacaacc
      121 gcttcccggc cttcgatggg aatctggtag cggcgcagaa ggcgcatgat gccggccggc
      181 gtggcggagc cgtaggcggg ctcgtccatg gccatgcgtc cgaagccgtg cgccgtaacg
      241 ccatcgacgt ccttatcgag agcgatgcgg tcgaaacagg cacgctcatc gatctgtttg
      301 ggaacgggat gctgcagcag aatgccgcgc acgcgcggat tgccgttgag ctcatcgatg
      361 gccccgagga gttccgcggt ggtggtcgtg ccggcgagat gaatgctgag ggattccatg
      421 ccgacgcggc ggcaggcgtt gcccttcatc ttgacgtagg tggctgaggc aggatcgtcg
      481 ccgacgagga tagtggcgag gatgggcggt tgctcgagat gaagacgctg gacgcgcaga
      541 gtgagttcgg cttcgagttc cttagagtag gccttgccgt cgagaaggat gggcataaag
      601 acaggttaca aggaacagga gccgggcaga ggagaccgat ccgcgggcgg cctggttgag
      661 gtggcccgcc gcggcacaac gtcatttgga ctgaacggcg agataccagg gagtcgcgag
      721 aggataaggc gagcctggcg cctgttctgg tgcggaaatc tgcgagtcct gaactgtgaa
      781 gacgtaaagc ttgcccggac tgatcccgcc cgttcccgtg tcgttgtcgg tgattgcata
      841 caggtggttg tggctgtccc agaacatctg cgcgatggga tcggtcgtca gcaagtcgcc
      901 atcggcggtc agggcagttc catcgaagtg aaagatttca agaccgccct tgcctccgac
      961 ggccagcaag gtgccggagg gggacatctt cagatcctcc ggagccgaga cctttgtggt
     1021 tggcatggtc gcgtaggtat cggtagtgct caggttcccg ctggcatcga ttgtgtaggt
     1081 ggccagctgg gctggtgtgc ccgaattctt gtagcaggga aactcggcga agacgaagtg
     1141 gtccgtgctg tccgaggcgg aaggtccggg ataaaatgcc tggaccatcg tctcctgcgc
     1201 cccaggcggt ggcgtgagga gcggcggagc cgtgtacgtc gagttctccg ccaggagccc
     1261 gttgctgtcg cgaatcatcg cggtcagact aaagatgtag catccgtcgt tgtaggccga
     1321 gtaggcgtac gtattgctgc catcgaaatg aacgcgtggg ctggtgttgt agccgatgtt
     1381 ctggatgcca agatacgtca ggcttccggc ggtcggatcg acagagaaag aagcaatccc
     1441 gttgttggtg ctgcacccgt attggcccac acctgcgtag agtgattgcc cggtgtgatc
     1501 gaattccagg tctcccacga tgccgcaggc attcgcctgg tactgggttt gctgagagta
     1561 atcgaattgc tgcgttcgag tcggcacacc gttcggaccg atcgaataag tgttgatatt
     1621 gggagtggca acggccgccg ccatcaggta actgccgttg gtcgcgacgg agttcacgtt
     1681 ctcattgaac ggcgaaccgg atacgggcct gagttgacca ttcatatctg ccgcgtacgc
     1741 cacgatctgg cgtggagaac tgggcgtgta gtcagccgag ttgaccacat acacatacgc
     1801 cgccacgctc ggggccggac tcgtggttcc tcctcctccg cctggatttc ccgtgggtgg
     1861 gggactctgg ggcgtaccgc cgttggacgt ggtcgtgcca cagccgctca gaagagccgc
     1921 gagcacaagc gctgcgtagg acgaagtcag gagccgctgg aatcgcatag aaacctcgtg
     1981 ggggaaagaa ctacaggcgc gaggagtctc acttcgagcg gagagcccac ttcacagggc
     2041 gcgttcacct gcgcgattcc ggcaaatcgc caggtcgatt gcccaggtgg aaacagaaaa
     2101 gcaatatcga aggccaggat catgctgcgg gacggcctcg gcgaacagcc gtccgcgagt
     2161 ttagatgact gtccaggaaa gcgagatgtg tcgcgtgcgg caagacctgt gcacggcagc
     2221 aaacaaaccg cctgagaact gcacccttaa gtctgttctg gtggctgatc tctgggctca
     2281 gatcgcaagc ggcaacgaaa tgggggcctc ggactcctgg cggacgcctg gactttcgag
     2341 cggcacacat ttttgtggct caacgagttc aggccgaggc aatccgtttg gcaacgatgg
     2401 cgtctgctgg aaaatcgatg acgccggaat cgtcagaagg agtgccaatc gtgtcatctc
     2461 ctgcctgagt gcggcgacgg tggcgagaat gtcattcccc gttggctcgc tgctgtgcag
     2521 catggccgac atcgcgattt gagaacccag ctccgcgcac agcgcccgca gtgtgtcccc
     2581 caactcgatc ttcgcggagg cactgatgcg cgtcgtgcag cgtgtggcca gccacaaatc
     2641 gatatcgctg gttcgcgcga tgatcgcttt cttattcgcg ccgtggggcc gccggacggg
     2701 aaggccgaaa tcctgttccc atcgttgcac ggtgcgcacg cctttgttca ggtattgtgc
     2761 gatctctttc caggacgtga gtatgcgaga cggacaactt gcaacttcga tgttcatgat
     2821 cgtcgtcttc gactggaact gacagggccc tgtgcgctac aacgtctggt gcgatgcact
     2881 gcggccgcga atgagttgca cgccccttca ttccagtcgc tttcgagcgc aattttttgg
     2941 cttcagttcg gtcgatggcg acctgccggc taaatggcgc ggatcaggcg aaccggaagg
     3001 gtgatcacag ccccaaccag atcgaggacc ccgtgaacgg cgacgccgag caggcggaag
     3061 ggaatcagaa gcagccaaac aatgggatac gcgatcagcg ccagaagcgc caggggccag
     3121 cagaggaaca gcagaatgca gaagagaagg aatttgatca tcgcgatgcc tcacttccca
     3181 c
//
LOCUS       DASZYN010000356         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_333795, whole genome shotgun sequence.
ACCESSION   DASZYN010000356 DASZYN010000000
VERSION     DASZYN010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333795"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VHE33_09130"
     CDS             complement(<1..863)
                     /locus_tag="VHE33_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97657.1"
                     /translation="MKSRRFSVCFAGLPLFAACLFLMHTAASFAQGTRLWNESRFEDL
                     ERGTPNGVAVTSDGYLVPGPESKLVVATPSTYVWAVAADKEGNAYLATGTPATVERVT
                     PEGKSTTLFTTREMSVQTVRVGPDGMVYAALLPSGKVYKLDPHVEGKNDDTATLVFDP
                     GTTQEKPKYVWDLAFDAKGRMYVATGAPAAVYRVVVGGKPEVFYKSDEEHIRALAFDK
                     AGNLIAGSDGTGLVYRIDAQGKAFVLFDAPKKEITSVVVAPDGTIYAAGAGNKGRGNL
                     PPLPVTGQASVT"
     gene            1037..>1611
                     /locus_tag="VHE33_09135"
     CDS             1037..>1611
                     /locus_tag="VHE33_09135"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HVT97658.1"
                     /translation="MLKRFRTRILLFLAVLGPGFITANVDNDPNGIFTYSQAGAQFGY
                     ALLWTMIPVTLALIVVQEMCARMGAVTGKGLSDLIREEFGLRLTIIVMVLLVIVNFGN
                     VIGEFSGIAGSLQLFHVSKYISVPVCAGLVWLLAVRGDYKTVEKVFLFSSMIYFTYII
                     AGVLAHPGWREAAIATVRLPKMSVLRDPGYV"
BASE COUNT          279 a          625 c          387 g          320 t
ORIGIN      
        1 gttacgcttg cctggcccgt caccggcagc gggggcagat ttcccctgcc cttgttgcct
       61 gctcccgccg catagattgt cccgtccggc gccaccacca ccgacgtaat ttccttcttc
      121 ggcgcgtcga acagcacaaa cgcctttccc tgcgcatcga tccggtacac cagtccggtc
      181 ccatccgatc ccgcaatcag attccccgcc ttatcgaacg ccagcgcccg gatgtgctcc
      241 tcgtcgctct tgtagaaaac ttcgggcttc ccacccacca ccacccgata caccgcggcc
      301 ggtgctcccg tcgcaacata catccgccct ttcgcatcga aggccagatc ccatacgtac
      361 ttcggcttct cctgcgtcgt gccgggatcg aacaccagcg tcgccgtgtc gtcgttcttc
      421 ccctccacat gcggatccag cttgtacacc ttgcccgagg gcaacaacgc cgcgtacacc
      481 atcccgtccg gccccacccg aaccgtctgg acgctcatct cgcgggtcgt aaacagcgtc
      541 gtcgactttc cttccggcgt cacgcgctcc accgtcgccg gtgtccccgt cgccagatac
      601 gcattccctt ccttgtccgc ggccaccgcc cacacatacg tcgacggtgt cgccaccacg
      661 agcttgctct ccggtcccgg caccagatat ccatcgctgg tcaccgcaac gccgttcggc
      721 gtcccacgct ccagatcctc aaagcgcgac tcgttccaaa gccgcgttcc ctgggcaaac
      781 gacgccgcag tgtgcatcag aaaaagacag gctgcgaaaa gaggcagccc cgcaaaacat
      841 accgaaaaac gacgtgattt catgctgatt gtcagtctac agaaacgttc ccgaagcggt
      901 ggcggcgcct atccaaagtc accctaaatc gccagacaca aatcctgtcc cctcgcctac
      961 actgaacatt cgccgctccc tctgcttccc gggcccgccg gctcggtatc ccgcgatctg
     1021 aaccggacgc ccccgcatgc tgaaacgctt ccgaacccgg atactgctct tcctcgccgt
     1081 gctcggcccg gggttcatca ccgccaacgt cgacaacgac cccaacggca tcttcacgta
     1141 ctcccaggcc ggggcgcagt tcggctacgc cctgctctgg accatgattc ccgtcaccct
     1201 cgcgctcatc gtcgtgcagg aaatgtgcgc gcgcatgggc gccgtcaccg gcaagggcct
     1261 ttccgacctg atccgcgagg aattcggcct ccgcctcacc atcatcgtga tggtcctgct
     1321 ggtgatcgtg aacttcggca acgtcatcgg cgaattctcc ggcatcgccg gcagcctcca
     1381 gctcttccac gtctcgaaat acatctccgt ccccgtctgc gccggcctgg tctggctgct
     1441 cgctgtccgc ggcgactaca agaccgttga gaaggtcttc ctcttctcct cgatgatcta
     1501 tttcacctac atcatcgccg gtgtgctcgc ccatcccggc tggcgcgaag ccgctatcgc
     1561 caccgtccgc ctgccgaaga tgtccgtcct ccgcgacccc ggctacgtct t
//
LOCUS       DASZYN010000357         1998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_656521, whole genome shotgun sequence.
ACCESSION   DASZYN010000357 DASZYN010000000
VERSION     DASZYN010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656521"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="VHE33_09140"
     CDS             complement(<1..1190)
                     /locus_tag="VHE33_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97659.1"
                     /translation="MGFDARELTPRVVQKITVLAAEIRSFKRCVIALREADVQTSAKT
                     VERIVHDIGRELAERRDAALSRNEALAQRPEEPPELAVVECDGGRIRCREPGHGPGVH
                     LDGKGWREDKNACLIRAKRQVFEEDPQPDPPACFCDPQHVAKIAETEALSVASLEPRA
                     LSERDQERDETPLEPPDWRPKCLVRTVLSSMACAAVFGAQMAREAKRRRFGEAAAKAF
                     LGDGLSWNWSIWKKRFRGFTPILDFIHPLSYLFLAAKAVHAQSQDAWDQYLAWMRGCW
                     LGEVGQVLEELCHWQTQLGEPDQATPENDPRQIVAKTIGYLRNNSMRMNYPDYRRQGL
                     PVTTAWMESLVKEINYRAKGTEMFWNDPEGAEAILQVRAAALSDDDRLVRHLRNRPGY
                     PFLRR"
     gene            complement(1732..>1998)
                     /locus_tag="VHE33_09145"
     CDS             complement(1732..>1998)
                     /locus_tag="VHE33_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97660.1"
                     /translation="TGRIGQAVLEDRFTDLMELAKLFQFAAEAVPQRALGTQFVQQYL
                     GLDERIGRDLGLYEQLSPATGNFLFGEQVCTFHEIAKTEPTSSF"
BASE COUNT          385 a          631 c          547 g          435 t
ORIGIN      
        1 cggcggagga acggatagcc gggacggttt cgaagatggc ggaccagccg gtcatcgtcg
       61 ctcagggccg cggcgcgaac ctgtaaaatc gcctcggctc cttcaggatc attccagaac
      121 atctccgttc cttttgcacg atagttgatc tctttcacca gcgactccat ccaagcggtg
      181 gtcacgggca ggccttgccg gcgatagtca ggatagttca ttcgcatgct gttgtttctc
      241 aggtagccaa ttgttttggc aacgatctgg cgaggatcat tttcgggtgt cgcctgatcc
      301 ggctcgccga gttgagtctg ccagtggcag agctcttcca ggacttggcc gacctcacct
      361 agccaacaac cgcgcatcca agcgaggtac tgatcccagg catcttgcga ttgggcatgt
      421 accgctttcg ccgccaggaa caggtagctg agcgggtgaa tgaaatcaag gatgggagta
      481 aagccccgaa agcgcttctt ccaaatggac caattccacg acaggccgtc acccaggaaa
      541 gccttggccg cggcttcgcc gaagcggcgg cgtttggcct cgcgggccat ctgggcaccg
      601 aatactgccg cgcaggccat gctgctcagc acggtacgaa ccaagcattt gggacgccag
      661 tcaggcggct cgagcggtgt ttcgtcccgc tcttggtctc tttccgagag tgcccgcggc
      721 tcgagcgacg ccaccgaaag ggcttccgtc tccgcaattt tcgccacgtg ttgcgggtcg
      781 caaaaacagg ccggcgggtc aggctgcgga tcctcctcaa agacctgccg cttcgctcga
      841 atcaggcagg cattcttatc ttctcgccaa cctttaccgt ctaaatggac acccggccca
      901 tgccccggct cacggcaacg gatgcggccg ccgtcgcact caacgacggc tagttccggt
      961 ggttcttcag gacgctgtgc caaagcctcg tttcggctta gggccgcatc ccgtcgctcg
     1021 gccaactcgc gtccaatgtc atggacgatt cgttcgacgg tcttggcaga agtttgcacg
     1081 tcggcctcgc gcaaggcgat cacgcaccgt ttgaacgaac gtatttctgc cgccaaaacg
     1141 gtaatctttt gcaccacgcg tggcgtcagc tcacgagcgt caaatcccaa cgtttcacgc
     1201 agagggaaaa aaagccctcc ggcaatggga gcaatcgccg gccagttcgt ccaactcgac
     1261 cggaccgtcg cccgacgtta cccttcgctt ttttagcttc agcggctgct gtctgccgca
     1321 tttcggacac ttggctgacg aaggctggtc cgcgagcagt tccctcatgt gcatctgctg
     1381 cacccggcgg ctgacctcct gtcccacctc atgggctgcg tcttcaatga cggagtaatg
     1441 gggaatgcct ttgatcctgc ccccggcacg atccaaggca accttcgcct ccgcggtttg
     1501 tctcagcaat tctgtaaatc tttcccgaag ccggctctct ctgtcgtcca tgattggatc
     1561 tccttaagcc taccgcggcc ttccctgccg ccggtactcg ccctacttta acgctcccaa
     1621 accaaattct cctattgagc ttttttgcac cccaccaaca agcacggaag ctggcgtgca
     1681 ccctcacggc gccgcccggc cggatggcta ttgcgtactc cggccagccc tttaaaatga
     1741 ggatgtaggt tctgtcttcg cgatttcgtg gaaggtgcaa acttgctcac cgaacaagaa
     1801 gtttcccgta gctggcgaaa gctgttcgta aagaccgaga tcacggccga tacgttcgtc
     1861 aaggccgagg tattgctgga cgaattgcgt gccgagagcc ctttgcggca ccgcctcagc
     1921 agcgaattgg aagagcttcg ccaactccat aaggtcggtg aaacggtcct ctaggaccgc
     1981 ttgacctatc ctgccggt
//
LOCUS       DASZYN010000358         8323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_333892, whole genome shotgun sequence.
ACCESSION   DASZYN010000358 DASZYN010000000
VERSION     DASZYN010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8323
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333892"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VHE33_09150"
     CDS             complement(<1..947)
                     /locus_tag="VHE33_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97661.1"
                     /translation="MAAVELPLGTFRDPAGSLRIAGDRVLREVRRECAEAALSFLQSS
                     TAKVWVAQGRLIDTRILHEDDSALILEHPRVFFPSYPWEWTPSAWIAAADLTLDLCEE
                     LLEHGLILKDATPLNILFRGARPVLVDVLSIEQRDPASPLWLAYAQFVRTFLLPLAAC
                     ESLGWPLAASMQNRDGYEPADLAPHLSRWQRWREPFRSLVTLPLMLEKSAPAPNTGLR
                     QPPEAALRALRSNLSRLRAHLRSIEQRLGSRRSASRWGNYASSADHYSSEDQEGKRRF
                     VQEALSAIAPRRVLDLGANSGVYSRIAAASGATVVAWDT"
     gene            complement(947..1201)
                     /locus_tag="VHE33_09155"
     CDS             complement(947..1201)
                     /locus_tag="VHE33_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97662.1"
                     /translation="MHLVHRTALAAFASIELAVLLVALTKPAYGYVDPGSGLLAVQVG
                     GSMLAGALFVLRSKLRRLFGLKSPNATSETEQSTQAESGE"
     gene            1427..2359
                     /locus_tag="VHE33_09160"
     CDS             1427..2359
                     /locus_tag="VHE33_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97663.1"
                     /translation="MSTRAESSLSANLQAAAQQAGLVVLSVDAGTDFNGQPTSRFRLA
                     MTPDASAERTLFLELSEAFNFHKPELLPEMTSHLREAASRLRNPRPDVYVTIAGLPLS
                     FASFGWPFHRSTSGADTYIVHGTVRLEDGTQSPLHALVSASMTVTFAEIVPAPEQPYA
                     ETFIYNAVRKTLDRGQLELLKSGNRQPVPVTTRYYSRWQKKFFFTDTDDNSRMEFLSL
                     KVYWLSGALGASQPVWIADPRDAQYLNTTAAELQRMAADLAAQGALKLDGEYASATPA
                     LLGRGEEYHAKLQAALAVTRPTFNEEMRAGHTNM"
     gene            complement(2452..2892)
                     /locus_tag="VHE33_09165"
     CDS             complement(2452..2892)
                     /locus_tag="VHE33_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97664.1"
                     /translation="MQSSTLQMDEPGCSRRWYHAIRTVKVAFFLLAATTALAAAQDLP
                     PGTNLRPWETVAIRVPDKNDQMKMHEQQQQQKQTRFAAVNLERKRQIAEDTAKMVELA
                     NELKQEVDKTDKDTMSINVIRKADAIEKLAKSIKEKMKATAGSS"
     gene            complement(3093..3944)
                     /locus_tag="VHE33_09170"
     CDS             complement(3093..3944)
                     /locus_tag="VHE33_09170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT97665.1"
                     /translation="MIDGRRSGTADRVAIRRASHALCDRPLIFDDPLAMRILSEEGAA
                     RAREEAAEPEQQPWLRGLRMFLAVRSRFAEEELAQAVGRGVRQYVVLGAGLDTFAYRN
                     PFPSLRVFEVDHPATQAWKRERLHHGQIAIPDSMRFAPVDFERDTLAHGLDAAGFRTD
                     EPAFFSWLGVVPYLTREAALATLGFIGGLPRGTGVVFDYAIPPESMGEAERQAFDALA
                     ERVARAGEPFRLFFDTEPLAAELHALGFTKIEDMDGAAIRKRWFGENADGPRPHGRSG
                     RLLCAWV"
     gene            complement(3951..5012)
                     /locus_tag="VHE33_09175"
     CDS             complement(3951..5012)
                     /locus_tag="VHE33_09175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVT97666.1"
                     /translation="MRWSRFLLTLLLGATGFAQQQLNLPPVSQESPASTASTISLNVM
                     VTDKSGHPIRGLTQADFTLLDDKQPAAIRSFTAHDLSTPQNDPQTMILLIDDVNANFS
                     VVSNVRMQIENFLHRNGGKLAIPVGILLLTDNGLETIAPPGTEGNALASVLHAKEGQL
                     HILSRASGFYGAEERTKISLDALTTLGKDLAQVNGRKLVVWIGPGWPIFDNPNVSFSS
                     QQQRNFFSGVVYLSGMLRQLGITLDSIDPLGPADSASPRSFLWESFIKPLTKPTKADP
                     GHLALQVFAVHSGGTVAVGSNDITGEIEKCAEDASAWYSIAFDPQKADSPNTWHDLAV
                     KLDKPGLKVRALNGYYAQP"
     gene            complement(5074..7872)
                     /locus_tag="VHE33_09180"
     CDS             complement(5074..7872)
                     /locus_tag="VHE33_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97667.1"
                     /translation="MAIRWFRPFLAVAVSLAAVTCAWADPAPFDLVGPKLEVKVTRAG
                     KSLPIAEVPNLMAGDHLWIKADLPKAQSAHYLMVAAFLRGSTNPPPENWFFKQETWDK
                     KAKEGLTVTVPEGAQQVLIFFAPETGGDFKTLMGAVRGRPGSFVRASQDLNQAMLDRS
                     RLDTYLAAVHRLDAADPSKLKDAAPLLARSLGMKIDDKCLDRMPGLQAACLTQGQDAL
                     ILSDGHSESIVEAMSSGPASDLAMQLAYTPQASYGFYSPYIGSVIDIARILETFHTAQ
                     FQYIPALATQQGEHIALLLNTPPSFHNPKSVLVTALPAVQAAQPPPLHPVDSKQNYCA
                     QKNSLVLAVDGAPLVFSTGYAHEMTLRLKKKDGKFVDESVQPDAMKGGLELEAKDLSA
                     GDFPNTIDASLHGYWGFEKYDGPTFRMENTHAQHWELAAADREALIVGREDTIHLSAD
                     DASCVDTILVKDSAGKELKADWKATSWDQLEVKLPLKEAHPGALQLEVKQFGTVTAQT
                     IALRAYAEAGHLDSFTLHAGDARGILKGSGLDGVAKLAVNGVAFAPGKADSSMGSDQL
                     PMIAKDVQEAENLKQGSSLKAQVTLTDGRTMTIPVMVGAPRPGVQLIGKSVQTSATSA
                     GSNIQLANQDELPQDAKLRFSVRAQSPASFGRSEKIEVATEDEAFSTTLSLDNGGMTL
                     ETSKVALATLDPDKAFGASAFGPLKFRVVEDSTAGDWQPLATLVRLPELHDLKCPPGD
                     DQQCRLSGSNLFLVDSVASDPEFAHPIQVPDGFPGYVLPVPHPEGGQLYLKLRDDPSV
                     VNQVTLAAEKLPPPPGESHAPRAPYHEGEAAPAPGAKGQTGSAMAPSAEPETGAAAND
                     QSAPPASAPHKDSATPHETKPSAPPSTAQQPQSAPEDSQASGEATTAPTPDAQAGSRA
                     GAAQSGTSAPTPQTTSPQ"
     gene            8035..>8323
                     /locus_tag="VHE33_09185"
     CDS             8035..>8323
                     /locus_tag="VHE33_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97668.1"
                     /translation="MDLSRRRFNRMLTSIGLAPAFSGFTAEGAITGSSGGGRDPQMLR
                     LSRNGWMPNNDHLPVLLYRGVLQNRGEDPAGACEAIFTRNGWAPQWRNGVYD"
BASE COUNT         1601 a         2648 c         2498 g         1576 t
ORIGIN      
        1 tcggtatccc aggcaacgac cgtcgcgcca gacgcggctg caatgcggga atagactccg
       61 ctgttcgctc ccaggtcgag cacccggcgc ggcgcaatcg cactcagggc ctcctgaaca
      121 aagcggcgct ttccctcctg atcctcagag ctgtaatggt cggcagacga cgcatagttg
      181 ccccatcgtg aagccgagcg gcggcttccc agacgctgct cgatgctccg caagtgagct
      241 cgcaagcggc tcaaattgct tcgcagcgcc cgcagcgccg cctccggcgg ctgccgcagg
      301 ccggtgttgg gcgcgggggc gctcttttcc agcatcagcg gcaacgtcac cagggaacgg
      361 aatggctcgc gccagcgttg ccagcggctc agatgcggcg cgaggtcggc cggttcgtaa
      421 ccatcgcggt tctgcatgga cgcggcgagc ggccagccca gagactcaca ggccgcgagg
      481 ggcagcagaa aggttcgcac aaactgtgcg tacgcgagcc acaacggact cgccggatcg
      541 cgctgttcaa tcgacagaac atcgaccagc accgggcgcg ctccgcgaaa gagaatgttg
      601 agcggcgtcg catctttgag gatgaggccg tgttccagca actcttcgca taggtccagg
      661 gtgagatcgg cggcggcaat ccacgcgctc ggcgtccatt cccagggata agaaggaaag
      721 aagacgcgag gatgttccag aatcagtgcg ctgtcgtctt cgtgaagaat ccgcgtgtcg
      781 atgagccgtc cctgcgccac ccacaccttt gcagtggaag actgcaggaa ggagagagcc
      841 gcctcagcgc actcgcggcg cacctcacgc agaacacgat cgcccgcgat acgcagcgag
      901 ccggccggat cgcgaaaggt tccgagggga agctcgacag ccgccactac tcgcccgatt
      961 cggcttgcgt gctctgctcg gtttcactcg ttgcgttggg agacttcagg ccaaacagac
     1021 ggcgcagctt cgagcggagc acaaacaaag caccggctaa catcgagccg ccgacctgca
     1081 cggccagcag acccgatcca ggatccacat atccataggc tggtttcgtc agggcgacga
     1141 gcaaaaccgc cagttcgatc gatgcaaatg cagccagggc agtgcggtga acaagatgca
     1201 cagggagcct ggctcccgcg ttgggggaat tctgacgcat tagcaactag acgatgaaaa
     1261 ttccgaaaaa gttgcgctgc cggcagggcg aatcgaggct gatttctcag gcaatccgcg
     1321 atccgaacgg ccactttctg ccattcggca ggctaagctg cggcgcaact gctccccgcc
     1381 atgagttccg gcagccatcg ccagccgata caatggaatg acaattatga gtactcgcgc
     1441 cgaatcaagc ctttccgcca atctgcaggc cgccgcgcaa caggcgggcc tcgtcgtgct
     1501 ctctgtcgac gccggaacgg acttcaatgg ccagccgacc agccgttttc gtcttgctat
     1561 gaccccggat gcctccgcgg agcggaccct gtttctggag ctgagcgagg ccttcaactt
     1621 tcacaagccg gaactgctgc cggaaatgac cagccacctg cgcgaggccg ccagccggct
     1681 gcgcaatccg cgtccggacg tctacgtcac gatcgcgggc ttgccattga gctttgccag
     1741 tttcggctgg ccgttccatc gctccacgtc gggtgccgac acctacattg tgcacggcac
     1801 cgtccgcctg gaggatggga ctcagtcgcc gctccatgct ttggtgtcag ccagcatgac
     1861 ggtgacgttt gccgagatcg tccccgcgcc ggaacagccc tatgcggaaa ccttcatcta
     1921 caacgcagtg cgcaagacgc tcgaccgcgg gcaactggaa ctgctcaaga gcggtaaccg
     1981 ccagccggtc ccggtcacga cgcgctacta cagccggtgg cagaagaaat ttttcttcac
     2041 ggatacggac gacaacagcc gcatggaatt tctgtcgctg aaggtttact ggctctccgg
     2101 cgctcttggc gccagccagc ccgtgtggat cgccgatccc cgcgatgcgc agtatctgaa
     2161 caccaccgcc gccgaactac agcggatggc ggcagacctg gctgctcagg gagcgctgaa
     2221 gctcgacggc gaatatgcct ccgccacgcc tgctctgctg ggccgcggcg aggaatacca
     2281 cgcgaagctg caggccgcgc tcgccgttac acgccccaca ttcaatgagg aaatgcgcgc
     2341 cggccacacc aatatgtaaa cgccgtggac agcagacatc ggtaatcgac cgacatcgca
     2401 gaggcacatg caaaagcagc atgaccccgc ggaaggctta gaagggacgg cttaactgct
     2461 cccggcggtc gccttcattt tctccttgat gcttttggcc agcttttcaa tcgcgtcggc
     2521 tttgcggatt acgttgatcg acatcgtgtc tttatcggtc ttgtcgactt cctgcttcag
     2581 ttcattcgcc agttccacca tcttcgcggt gtcttcagca atttgccgtt tgcgctccag
     2641 attcacggcg gcgaacctgg tctgcttctg ctgttgctgc tgttcgtgca tcttcatctg
     2701 atcgttcttg tccgggaccc ggatggcaac ggtctcccac ggccggagat tcgtaccggg
     2761 aggaagatcc tgagccgcgg ccagagccgt agtggcggcc aacaggaaaa aggctacttt
     2821 gacggtgcgt atcgcgtgat accagcgtcg gctgcatcct ggttcgtcca tctggagagt
     2881 actgctttgc ataaggaact cctcatgagg ccgagagcaa ccgctcgtat atggatgaga
     2941 acctattctc ccacgcacag agcagaaaga cgagccgtaa ggtgctcggg ggaagcggac
     3001 ttccaaggtg gatgcaatgc ctgcactctg tggatgccgg aattgcttcg ttttatgaac
     3061 ggaccggctg gatcctggga cacgatcaga tctcataccc acgcgcacag cagccggccc
     3121 gagcgtccgt gcggacgcgg tccgtccgca ttctcgccga accagcgctt tcgaatcgcc
     3181 gcgccatcca tgtcctcgat cttcgtgaac cccagtgcgt gcaactccgc cgcgagcggc
     3241 tccgtatcga aaaagagccg gaagggttcg ccggcgcgag ctacccgctc cgctaaagca
     3301 tcgaaggcct gccgctccgc ttcgcccatg ctctcgggcg gaatggcata gtcgaagacc
     3361 acaccggtcc cacgcggaag gccgccgata aagcccagcg tcgcgagcgc cgcttcgcgc
     3421 gtgagatagg gcacaacgcc cagccacgaa aagaatgccg gctcatcggt ccggaacccg
     3481 gccgcgtcca gcccatgcgc cagcgtgtcg cgctcaaaat cgaccggagc aaagcgcatc
     3541 gaatcgggaa tggcgatctg cccatggtgc agccgctcgc gcttccacgc ctgggtagcg
     3601 ggatgatcga cctcgaagac gcgcaggctg ggaaatggat tgcgataagc gaaggtatcc
     3661 agccccgcgc cgagaactac atactggcgc acgccccgcc cgaccgcctg cgccagctct
     3721 tcttcggcaa agcggctgcg cacagcaaga aacatcctca ggccgcgcag ccagggctgc
     3781 tgctcgggtt ctgccgcctc ctcacgcgct cgggccgcac cttcttcgct gagaatgcgc
     3841 atggccagcg gatcgtcaaa gatcagcggc cgatcgcaaa gcgcgtgcga tgcgcggcgg
     3901 atcgccacgc ggtccgcggt tccactgcgc cggccgtcaa tcatggggca tcaaggttgc
     3961 gcataatagc cgttgagggc gcgcaccttc aggcctggct tgtcgagttt cactgcaaga
     4021 tcgtgccacg tgttgggtga gtccgccttt tggggatcga acgcgatcga gtaccatgcc
     4081 gatgcgtctt cagcgcattt ctcgatttcc ccggtgatat cgttgctccc cacagcgacc
     4141 gttccaccac tgtggaccgc aaagacctgc agcgccaggt ggccgggatc cgctttcgtc
     4201 ggcttcgtca gcggcttgat aaagctctcc cacaggaagc tgcgcggact cgccgaatcg
     4261 gccgggccga gaggatcgat ggagtcgagg gtgatgccaa gctggcgcag cattcccgaa
     4321 agatagacga ctccgctgaa gaagttccgt tgctgctgcg agctgaatga cacgttagga
     4381 ttgtcgaaga tcggccagcc gggcccaatc cacacaacga gtttacggcc gttcacctgg
     4441 gccagatcct ttcccagcgt ggtcagtgca tccagcgaga tttttgttcg ttcctcagcg
     4501 ccgtagaaac cagaggcgcg agagaggata tgcagttggc cttccttcgc gtgtaaaacg
     4561 ctggccaggg cattgccctc ggttccgggc ggcgcaattg tctccaggcc gttgtctgtc
     4621 agcaggagaa ttcccacggg aatcgcaagc ttgccgccgt tgcggtggag gaaattttca
     4681 atctgcatgc gaacgttgct caccacgctg aaattcgcat tcacgtcgtc gatcagcagg
     4741 atcatcgtct gcggatcgtt ttgcggcgtg gaaagatcgt gagcggtgaa cgagcgaatg
     4801 gccgcgggct gcttgtcgtc gagcagggtg aaatcggcct gagtcagccc gcgaatcggg
     4861 tgaccggact tatcggtgac cataacgttc aggctgatgg tgctcgccgt tgaagcgggc
     4921 gattcctgag acacaggcgg caaattgagc tgctgctgtg cgaatccggt agcgcccagc
     4981 aacagcgtca gcaggaacct gctccaacgc atcgacaaac caggacctcc tgcgttcacg
     5041 acagcaatct gcagcgcgcg cacaaagcat cggctattgc ggcgaagtcg tctgcggcgt
     5101 tggcgcgctc gtcccgctct gcgcagcgcc tgcacggctg cctgcctgcg catcgggtgt
     5161 cggcgccgtt gtcgcttctc cggatgcctg cgaatcttcc ggtgcgctct gcggctgctg
     5221 agcagtggac ggcggagccg acggtttggt ctcatgcggt gtcgccgagt ccttgtgcgg
     5281 cgcagaggct gggggcgcgg actggtcgtt tgccgctgct cctgtttccg gctctgccga
     5341 tggcgccatg gcagatcccg tctgtccctt cgcgcccggc gccggtgcag cctcgccttc
     5401 gtgataggga gcgcgcggcg catgcgactc acccgggggc ggcggcaatt tctccgcagc
     5461 cagtgtcacc tgattcacga ccgatggatc gtcgcgcagc ttcaggtaaa gctgcccgcc
     5521 ttcgggatgt ggaacgggca gaacgtatcc gggaaaacca tcgggaacct gaatgggatg
     5581 cgcgaactcg ggatcgctcg ccactgaatc caccagaaac agattcgatc cgctcaaccg
     5641 gcactgctga tcgtctcccg gtgggcactt caggtcatgc agttccggca ggcgcaccag
     5701 cgttgccagc ggctgccagt ctccggccgt gctgtcctcg acgacgcgaa acttgagcgg
     5761 accgaaagca gacgctccaa acgctttgtc gggatcgagc gtcgccagag ccactttcga
     5821 cgtctcgagc gtcatgccgc cgttatcgag gctgagcgtg gtcgaaaagg cctcgtcttc
     5881 ggtggccact tcgatttttt cgctccgacc gaacgacgcg ggcgactgcg cgcgcacgct
     5941 gaagcgcagc ttcgcatcct ggggcagctc atcctgattc gccagctgaa tattgctgcc
     6001 tgcgctggtg gccgaggtct gcacgctctt gccgatcagc tgcactcccg gacgcggcgc
     6061 tcccaccatc accggaatcg tcatcgtccg gccatcggtg agcgtgacct gggctttgag
     6121 agaagacccc tgcttcaggt tctccgcctc ctgcacgtct ttcgcgatca tcggcaactg
     6181 atcactgccc atcgacgaat cggccttccc aggggcgaac gcgacgccgt tcacagcgag
     6241 cttggccacg ccgtccagac cgctgccctt gagaatccca cgggcatcgc cggcgtggag
     6301 ggtaaagctg tccaggtgtc ccgcctcggc ataagcgcgc aatgcaattg tctgcgccgt
     6361 taccgtaccg aactgcttca cctcaagctg cagagctccc ggatgcgcct ccttcagagg
     6421 cagcttcact tcgagctggt cccaactcgt cgctttccag tcggccttca gttctttccc
     6481 tgccgaatct ttcaccagga tggtgtcgac gcaactggca tcgtctgcag aaagatgaat
     6541 cgtgtcctcg cgccccacga tcagggcctc acgatccgct gcggccagct cccagtgctg
     6601 cgcgtgcgta ttttccatgc ggaaggtcgg gccgtcgtac ttctcaaatc cccagtagcc
     6661 atgcagcgag gcgtcaatgg tgttgggaaa atcgcccgcg ctcagatctt tggcttcgag
     6721 ttcgagcccg cctttcatcg catccggctg aacggactca tcgacgaatt tcccgtcctt
     6781 cttcttcagg cgcagcgtca tctcatgcgc gtatccagtg gaaaaaacca gcggcgctcc
     6841 atcgacggcc aacaccagcg aattcttctg tgcgcagtag ttctgcttcg agtccaccgg
     6901 atgcagcggc ggaggctgcg ccgcctgcac ggccggaagc gccgtgacca ggaccgactt
     6961 tggattgtga aacgacgggg gcgtgttcaa caggagcgcg atatgctcac cctgctgcgt
     7021 cgccagggcc ggaatgtact ggaactgggc cgtgtggaat gtctcgagga tgcgcgctat
     7081 gtcgattacc gacccgatgt acgggctgta gaatccgtag ctcgcctgcg gtgtgtacgc
     7141 cagctgcatc gccagatcgc tcgccggtcc ggagctcatc gcttccacaa tcgactcgct
     7201 gtgtccgtca ctgaggatca gcgcatcctg accctgggtc agacacgccg cctgcagccc
     7261 cggcatccgg tccaggcact tgtcgtcgat cttcatgccc aggctgcgcg ccagcaacgg
     7321 tgccgcatct ttgagcttcg acggatcggc agcatcgagg cggtgcacgg ctgccagata
     7381 ggtgtcgagc cgcgaccggt cgagcatggc ctgattcaga tcctgcgaag cccgcacgaa
     7441 cgatcccggc cgtccgcgca ccgcgcccat cagcgttttg aagtcgccgc ccgtttccgg
     7501 cgcgaaaaaa atgagcacct gctgcgcgcc ttcgggaacc gtgacggtga gcccttcctt
     7561 cgccttcttg tcccaggttt cctgtttgaa gaaccaattc tccggcggtg ggttcgttga
     7621 accccgcagg aaggccgcca ccatcagata gtgtgccgac tgggccttcg gcagatccgc
     7681 cttgatccag agatgatctc cggccatcag gttggggact tcagcgatgg gaagcgactt
     7741 gcccgcgcgc gtgaccttca cctcgagctt cggaccaacc agatcgaagg gagccggatc
     7801 ggcccacgcg caggtgaccg cggccaaaga cacagctacg gctaaaaacg gacgaaacca
     7861 ccgaattgcc atatcagtaa ctattctagg ccgcatggac gccagaaaaa ggagagaaaa
     7921 taccttgcca gcagagacgt tattgacttc aaaaggttat tgacacaaag aggaatcgca
     7981 gttactacag tgctcgaacc agcgttcgct attttggcgc catccaaaga gaacatggac
     8041 ctgagccgcc gccgcttcaa tcgcatgctg acctccatcg gactcgcacc ggcgttttcc
     8101 gggttcactg ccgaaggcgc gattacaggc agcagcggtg gcgggcgcga tccgcagatg
     8161 cttcgccttt cgcggaacgg atggatgccg aataacgacc acctgccggt actgctctat
     8221 cggggcgttt tgcagaaccg cggggaggat ccggctggcg catgcgaggc catcttcacc
     8281 cggaatggct gggcgccgca gtggcgcaat ggcgtctacg act
//
LOCUS       DASZYN010000359        13649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_466031, whole genome shotgun sequence.
ACCESSION   DASZYN010000359 DASZYN010000000
VERSION     DASZYN010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 13649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13649
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466031"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VHE33_09190"
     CDS             complement(<1..1088)
                     /locus_tag="VHE33_09190"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase family protein"
                     /protein_id="HVT97669.1"
                     /translation="MLSAAATDSLLTHVQTLAFFFVKNDLRHYLRTHYGDHTFQNLYH
                     WQFFSFDTALLTPYFAVMIVLALYGFHRYTLVWLYYRNRKNATHEPPARFEQLPRVTI
                     QLPIFNEQFVVDRLIDSICRIDYPRDRLEIQVLDDSTDETKEVAQAIVERYRALGNPI
                     VYLHRTNRVGFKAGALDEGLHTATGEFIAIFDADFTPPSDWLMRVVHHFAEPEIGMVQ
                     TRWTHLNRDYSFLTQVEAILLDGHFVLEHGARARTGVFFNFNGTAGMWRRCVIDQAGG
                     WQHDTLTEDTDLSYRAQLIGWKFKYLPDVACPAEVPIEMTAFKTQQQRWAKGLIQTSK
                     KILPRLFRSDVPLRVKIEAWYHLTANIS"
     gene            complement(1247..2017)
                     /locus_tag="VHE33_09195"
     CDS             complement(1247..2017)
                     /locus_tag="VHE33_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97670.1"
                     /translation="MYFRKFSGYALYPLLAFLLLVPSAIRAQDSSSQQPAPAPQTQPS
                     DQTPLQPSTQNQAQVLREAQARVNARRKVRIRQIIQDTYSHKYELNFGGGSLRFTPGN
                     SLQRINEAGWIVDFTDYFHGDLGIAADFRGYYGTAYTGTNPYSVFNPSISQYTFMGGP
                     RYRFFKGQHWGWTAEGLAGAGHGNFGTGTHGLPPELVGLYKDSTVLNFLGGVSADWNL
                     SPALALRFSANEVVTNYGSSFQENLGGNLGLVYRFGRK"
     gene            2225..2806
                     /locus_tag="VHE33_09200"
     CDS             2225..2806
                     /locus_tag="VHE33_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVT97671.1"
                     /translation="MRFLLGLVVGLLILPLCGVIYFRMGHPPVAVADVPFPFEKQIVH
                     VPLSARIESEMQKSAPIEASGTNLTAGAQIYREQCAACHGLYGRPSQYAAHMYPKAPQ
                     LWAPHRNGVVGVSDDPVGETQWKVMNGIRLTGMPAFGKVLNETQIWQVSVLLKNAGQP
                     MPADVTKLLTTPVDYGVGAAVVATPVPAQPTEH"
     gene            3031..3369
                     /locus_tag="VHE33_09205"
     CDS             3031..3369
                     /locus_tag="VHE33_09205"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HVT97672.1"
                     /translation="MVAIALLSDFGVEEADDLDLSGAWKLSQLSELPIGTVGVVESLD
                     LPSEIQHHLMHMGFVPEARVMVVRRAPAGDPTVYSIDGFEVALRRDTAGAIRVREYIR
                     EDQANHTRQG"
     gene            3366..5267
                     /gene="feoB"
                     /locus_tag="VHE33_09210"
     CDS             3366..5267
                     /gene="feoB"
                     /locus_tag="VHE33_09210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HVT97673.1"
                     /translation="MSDCCGTTATIEVPSEPRVAGRIRTVALVGPPNSGKSTLFNRLT
                     GLRQKVANYPGVTVEQRVGRLNGIGRGDLTLIDLPGIYGLDSYSEDARVAVEVLRGEM
                     PGTPMPDAILLVLDSLQLRRQLMLAAPVLALGLPTLVLLNMSDLMEARGGRVDTLALA
                     QELGVPVAKISATRGVGLDSVTHFLNRKAEAEKPVTAEGTIELPVMGNPRSYRQWAHG
                     VSTRTKYKAPLSNTWTKRIDGVLLHKIWGPLVFLAVVFAVFQVVFTIGQPLSDAFGDV
                     LNMGGDKIGALLGHAWVESLLIDGVWRGVASVLVFLPQILLLFLFIGVLEDSGYLARA
                     ALIADRVMRSIGLNGKAFIPLLSAYACAVPAIMATRTIENKRDRFATILVAPFMTCSA
                     RLPIYMLMIAAFIPNTPLLGDFFGLRAAVMLSLYFLGFVAALATARLLKSSVLKASSA
                     PFILELPQYRLPTVRSLSLRLVDRGKVFLRQAGTVILAVTLVLWVLSHIPFHTDLTTS
                     VIGRLGHWIEPAIRPLGFNWKIGIGLLTSVVAREVIVGTLGTLYGADPATQSLGLQAA
                     LRHDLTLGGAMALVVFFAFALQCTSTLAIVRRETNSWKWPALQFVYMGCLAYLMALLT
                     NQAILHFVR"
     gene            complement(5395..6657)
                     /locus_tag="VHE33_09215"
     CDS             complement(5395..6657)
                     /locus_tag="VHE33_09215"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HVT97674.1"
                     /translation="MQNFPHLPPDAVKIVLVLFLSFLIGLEREDYKSQPGRYSFGGVR
                     TFPLIGLLGYSLALLSGNQLLPLTIGFLVVAGFLLLSYWHKLSQPEAAGVTTEMSGLT
                     TFVVGALVCYGHFWIATTLSVASLLLLDLKVALEKLAGRVAPREILTFAKFLLLTGVI
                     LPVLPRQEFTRFHINPFKTWLVVVAISAISYGSYILQKITKSQGGVVVAALLGGAYSS
                     TVTTIVMARRSKQEEHPHLFSGGILIASGVMYVRLAVLLALFNRQMMALVDLPFLVLA
                     ALAILTGWLWTRRADPTAQSVQREFEPSNPLELGAAFLFAAIFLAMLIATQLAVTYVG
                     RTGVNTLAAIMGFADVDPFILGMTQAAGTLILAKTAAAGALIAASSNNIAKGIYAWSF
                     AHHRTGLQSFIFLVSLALLGLIPLFWIL"
     gene            6873..7670
                     /gene="otsB"
                     /locus_tag="VHE33_09220"
     CDS             6873..7670
                     /gene="otsB"
                     /locus_tag="VHE33_09220"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HVT97675.1"
                     /translation="MANSYSKLSLYPFFETLRAVGRSVLALDYDGTLAPFRQNPMEAV
                     PYPGVREVIEKILGTGKTRVVLISGRRAEEVRDLLGVRPAPEIWGVHGRQRLGADGRS
                     MIAPLAEREEEALEAAAAWVEHCGYGVRTELKPGSLAVHWRGLTAQEIGRASAATEAA
                     LWPIADRAGMCLLGFDGGVEIRTREPNKGTVVRALLGEQRDPIPFAFLGDDKTDEDGF
                     AVLRGTGALSVLVRPEWRATAAEVWLRPPEELLEFLTAWAESGEVKR"
     gene            7667..9133
                     /locus_tag="VHE33_09225"
     CDS             7667..9133
                     /locus_tag="VHE33_09225"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HVT97676.1"
                     /translation="MTVAAGRRAKAGRRARGRKRQGTLILVSNRLPASLEAGPDGPIV
                     KPSAGGLVTALRPVLKARNGFWIGWAGKGSESEIEKLVREAGAELGCQMIPVSLNAAE
                     RQGFYAGFSNEIVWPLFHDLQSRCNFDPTYWKAYVTVNRKFAEAVADVTKPDSMVWVH
                     DYHLMLCGEFLRQQGLPSRMGFFLHIPFPPLDIFEKLPWRSQILRALFQYDVVGFQTP
                     RDLRNFLGCVRRLLPDLDFHRTDEDVQATVGGHQTVLKSFPIGIDFDEFCGQAQRSEV
                     VARAEQIRKDMRGVQIVLGVDRLDYTKGVPERLRAVRHLLESRRGMRQKISLVQIVVP
                     SREDIPMYRELKLEIETLVSQINGQFGDPGWTPVHYMHRSVDRTELLALYRAADIALI
                     TPLKDGMNLVAKEFCAAQTGAPGVLVLSEFAGAAEQLQNGAILVNPYDFGSVAEAVNE
                     AFHMPLAERAKRMRKLRRKLQYADVFRWCEEFWEFLEG"
     gene            9498..9896
                     /locus_tag="VHE33_09230"
     CDS             9498..9896
                     /locus_tag="VHE33_09230"
                     /inference="COORDINATES: protein motif:HMM:NF021388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HVT97677.1"
                     /translation="MAEEGKGDAAEWQELTRLTQGQPVVVERVRLVGKNIAIEGSFEL
                     PQLARLNAEDQVFVVAFLRSHGSIKEMEQVFGVSYPTIKARLTRIAGSLEFVETDPGP
                     PRAEVLERLARGEIDATEAIRALEGMKGRD"
     gene            9921..10187
                     /locus_tag="VHE33_09235"
     CDS             9921..10187
                     /locus_tag="VHE33_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97678.1"
                     /translation="MIPSLAVVRVENPDWRWGGVRLWLPLVLVYIPLLILSPLILLVV
                     VVACWLGRVRPWRAIAGFWELFCSLAGTDVRVQAEKNRVLVRIL"
     gene            10239..10478
                     /locus_tag="VHE33_09240"
     CDS             10239..10478
                     /locus_tag="VHE33_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97679.1"
                     /translation="MKQERKQILEMVIAGRMTPLEAERLLLVWNEERESRWIFGACAA
                     PAVVNLAHAVWLQMAGSGGIHTAAGVVSWILGGVR"
     gene            10475..10930
                     /locus_tag="VHE33_09245"
     CDS             10475..10930
                     /locus_tag="VHE33_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97680.1"
                     /translation="MSEGRKQILEMLAAGKINANEAERLLVALEPGSGSGPEGGAVDG
                     KTGKKKPKYLRVLVEADESMTGLKGGATTVNVRVPMQLLRAGVRLAGLIPQQAHQQLD
                     QALNRHGVPLTLSQIKPENLEELIDHLEDLTVDVDGKDGNTTKVKVFCE"
     gene            11099..11791
                     /locus_tag="VHE33_09250"
     CDS             11099..11791
                     /locus_tag="VHE33_09250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT97681.1"
                     /translation="MTNVTATVTRTMAMPKPHRDRGIEGRMAQWYAATTAKSMGDFRE
                     LARRIARELPQGGSVLEIAPGPGYFCIELAKLGPYAIRGVDLSEAMVKIARAKAEEAG
                     VQVDFERGNAANLPYPREAFDFLVCRAAFKNFAQPVRALREMCRVLKPGGRGLIIDLR
                     RNAGIAEVNEHVDTMGLSALNRIITKLTFRWMLLKSAYAREHFEQMLAKTAFGEFEVI
                     ESGIGLEVWMTK"
     gene            12490..12849
                     /locus_tag="VHE33_09255"
     CDS             12490..12849
                     /locus_tag="VHE33_09255"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HVT97682.1"
                     /translation="MKMILAASAAAFVAIGSAPLNFVATQAAPQRIEVTAKRFSYEPE
                     TVTVKKGELVELVLKSEDVPHGLKFRELGVDVKAKKGKTGEVEFTPEKTGDFVGHCSV
                     FCGMGHGSMHLTLHVVE"
     gene            12852..13325
                     /locus_tag="VHE33_09260"
     CDS             12852..13325
                     /locus_tag="VHE33_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVT97683.1"
                     /translation="MRRGREVGVAAVAVAVMLGAGCKATPPNAFETRVMTMAKHKMLV
                     GNKDAKNPMAANAETIADGKGAFGHYCVACHGMDGQNTGVPFVMSPPAPSLASPDVQS
                     YTDGQLKWVIENGIWPSGMPGSKGTLSDDEQWSIVLFLRHLPPVGSVGEPEMYTH"
     gene            13334..>13649
                     /locus_tag="VHE33_09265"
     CDS             13334..>13649
                     /locus_tag="VHE33_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97684.1"
                     /translation="MRIRRQAGVVVLAALAAGNLVLRAQSTAGAGNTVTQSSGNGAWI
                     DPDDNARAREDALGHNQGPGSGATASAGLALTARQIFAVLDEKPEVVIELKTQLAEQL
                     QQQ"
BASE COUNT         2717 a         3191 c         5133 g         2608 t
ORIGIN      
        1 tagctgatgt tggcggtgag gtgataccag gcttcgatct tgacgcgcag gggcacgtcg
       61 gagcggaaga ggcggggcag gatttttttc gaagtctgga tgaggccttt ggcccagcgc
      121 tgctgctggg ttttgaaggc ggtcatctcg ataggaacct cggcggggca ggcgacgtcc
      181 ggcaggtatt tgaatttcca gccgatgagc tgggcgcgat agctgaggtc ggtgtcttcg
      241 gtgagggtat cgtgctgcca gccaccggcc tgatcgatga cgcatcggcg ccacatgccg
      301 gcggtgccgt tgaagttgaa gaaaacgccg gtgcgggcgc gggcgccgtg ctcgagtaca
      361 aagtgcccgt cgaggaggat ggcttcgacc tgggtgagga agctgtagtc gcggttgagg
      421 tgagtccagc gagtctggac catgccgatt tcgggttccg caaagtggtg gacgacgcgc
      481 atgagccagt cggagggagg cgtgaagtcg gcgtcgaaga tggcgatgaa ttctccggtg
      541 gcggtgtgaa gtccttcgtc gagggcaccg gctttgaatc ccacacggtt ggtgcggtga
      601 aggtagacga tcggattacc gagagcccgg tagcgttcga cgatggcctg cgcgacttct
      661 ttggtttcgt cggtggaatc gtcgagaacc tggatttcga gccgatcgcg gggatagtcg
      721 atcctgcaga tggaatcgat gaggcggtcg acgacgaact gctcgttgaa gatggggagc
      781 tggatggtga cgcgggggag ttgctcaaaa cgtgcgggcg gctcgtgggt ggcgtttttc
      841 cgattgcggt agtagagcca gaccagggtg tagcgatgga agccgtacag agcgaggacg
      901 atcatcaccg cgaagtaggg agtgaggagc gccgtatcga aggagaagaa ctgccagtgg
      961 tagaggttct ggaaggtgtg gtctccgtag tgggtgcgga ggtagtggcg gaggtcgttc
     1021 ttaacgaaaa aaaaggcgag ggtctggacg tgggtgagca gggagtcagt agccgccgcg
     1081 gagagcatga aggaccagcg tatagcggat agcgtttagc gtatagaggc gaggggaagc
     1141 ccgagcctgg gagcctgtgc gaaagatcgc gctgcgcgcg atgcccaccc tgttcgccaa
     1201 caacagacga acagaatggg gcacccggag ttacagatgg aatttcttat ttacggccga
     1261 agcggtagac gagtccaagg ttgccgccga ggttttcctg gaagctggag ccgtagttgg
     1321 tgacgacttc gttggcggag aagcggaggg cgagggcggg actgagattc cagtcggcgg
     1381 aaacgccgcc gaggaagttg aggacggtgg agtccttata gagtccgacc aactcgggag
     1441 gaaggccgtg ggttccggtg ccgaagttgc catggcctgc gccggcaagg ccttcagcgg
     1501 tccagcccca gtgctgacct ttgaaaaagc ggtagcgggg gccgcccatg aaggtgtact
     1561 ggctgatgct gggattgaag acggagtagg gattggtacc cgtgtaggcc gtgccatagt
     1621 agccgcggaa gtcggcggcg atgccgaggt cgccgtggaa gtagtcggtg aagtcgacga
     1681 tccagcccgc ctcgttgatg cgctggaggc tgttgccggg agtgaagcgg agggaaccgc
     1741 ccccgaagtt cagctcatat ttgtggctgt aggtgtcctg aataatctgg cggatgcgga
     1801 ccttgcggcg ggcgttgacg cgggcctggg cttcgcgaag gacctgggcc tgattctgcg
     1861 tggacggttg cagaggggtt tggtccgagg gctgggtctg tggggcaggc gcaggctgct
     1921 gggaggaaga atcctgtgcg cggattgcgg agggaacaag gagaaggaac gcgaggagtg
     1981 gatacagggc gtatccggag aacttgcgaa aatacatgaa cctgggtgcc tccactgctt
     2041 agacggaaaa atacagggta cagggaacag ggtacaggga accgggtgca gggtacaggg
     2101 atgggaaccg gggcttggcg ttcggggcgc ggtgtaccgg agtggggagg attgcggaat
     2161 cttcatcggc atgcaaggat gccggggaag agtgcgatta ttttgggatt gagggttgag
     2221 acggatgcga ttcctgctgg ggctcgtggt tgggctgttg attcttccgt tgtgcggggt
     2281 gatttatttc cgcatgggac atccgccggt ggcggtcgcg gatgtgccat ttcctttcga
     2341 aaagcagatc gtgcatgtgc cgctgtctgc gcgcatcgaa tccgaaatgc aaaagagcgc
     2401 gcccatcgag gcaagcggga cgaatctgac agccggggcg cagatttatc gcgagcagtg
     2461 cgctgcatgc catggcttgt atggacggcc gtcgcaatat gcggcccaca tgtatcccaa
     2521 agcgccgcag ttgtgggctc cgcatcgcaa tggtgtggtc ggcgtgagcg acgatccagt
     2581 gggtgagacg cagtggaaag tcatgaatgg gattcggctg acgggaatgc cggcctttgg
     2641 caaggtgctg aatgagaccc agatctggca ggtgtcggta ttgctgaaga atgccgggca
     2701 gccgatgccg gcggatgtta cgaaactgct gacgactccg gtggactacg gagtcggagc
     2761 cgcggtggtc gccacgccgg ttccggcaca accgacggag cactgacgaa cgagcgttca
     2821 ggtttcagcg atcggcgttc cgtccggatc agtaaatgtt tcggcttccc aaccgccgat
     2881 ggatgggtct gcaaagccaa accacaattt acgaagggtt tccacaaaag gaacccctgg
     2941 aacgagtgcg cgcggcgtgt gacctgaatc acggaaacgc cggagcggcc gatcttatgc
     3001 ttgccactac gtgggcaata taggagcgaa atggtcgcaa ttgcgctatt atcggatttt
     3061 ggggtcgaag aagcggacga tctggacctg tccggagcat ggaagttgag tcaattgagc
     3121 gaattgccca tcgggacggt aggagtcgtg gagtcgctcg atttgccgag tgagatccag
     3181 catcacctga tgcatatggg gtttgttccc gaggcgaggg tcatggtggt gcggcgggcg
     3241 ccggccgggg atccaacggt gtattcgatc gacgggtttg aggtggcgct gcggcgtgac
     3301 acggccgggg cgattcgggt tcgcgaatat atccgcgaag atcaggcgaa tcacacgagg
     3361 cagggatgag cgattgctgc ggcacgacgg cgacgattga ggttccgagc gaaccgcgag
     3421 ttgccggccg aattcgaacg gtggcgctgg tgggtccgcc gaattcgggc aagtcgacac
     3481 tgttcaatcg gctgacgggg ctgcggcaga aggtagcgaa ttatcctggt gtgacggtgg
     3541 agcagcgggt aggacggctg aacggcatcg gccgcggtga tctgacgctg atcgatctgc
     3601 ctggaatcta cggactggac tcgtattcag aagatgcgcg cgtggcggtt gaggtgctcc
     3661 gcggcgagat gccgggaaca ccgatgccgg acgcgatcct gctggtgctg gactcgctgc
     3721 aactgagaag acaattaatg ctcgcagcgc cggtgctcgc gctggggctg ccaacgctgg
     3781 tgctgctgaa catgagcgac ctgatggagg cgcgcggcgg gcgcgtggac acgctggcgc
     3841 tggcgcagga gttgggcgta ccggtagcga agatcagcgc gacgcggggt gtcgggctcg
     3901 attcggtaac gcacttcctg aaccgcaagg cagaagctga gaagccggtg acggccgaag
     3961 gaacaattga actgccggtg atggggaatc cgcggtcgta ccggcaatgg gcgcacggtg
     4021 taagcacgcg gacgaagtac aaggcgccgt tgtcgaacac gtggaccaag cggatcgacg
     4081 gcgtgctgct gcacaagatc tggggaccgc tggtatttct ggcggtggtg tttgcggtgt
     4141 ttcaggtggt gttcacgatc gggcagccgc tgagcgacgc gtttggcgac gtgctgaaca
     4201 tgggtgggga caagattggg gcattgctgg gacacgcgtg ggtggagtcg ctgctgatcg
     4261 atggggtatg gcgcggcgtg gcgtcggtgc tggtcttttt gccacagata ctgttacttt
     4321 ttcttttcat tggggtgctg gaggattccg gatatttggc gcgggcggcc ctgattgcgg
     4381 atcgcgtgat gcggtcaatc gggctgaacg ggaaggcatt tatcccgctg ctgtcggcat
     4441 acgcttgcgc ggtgccggcg atcatggcga cgcgcacgat tgagaacaag cgcgaccggt
     4501 tcgcgaccat tctggtagcg cctttcatga cctgctcggc gcggctgccg atttacatgc
     4561 tgatgatcgc ggcgttcatt ccaaatacac cgttgctggg tgacttcttt ggattgcggg
     4621 cggcggtgat gctgtcgctg tatttcctcg gattcgtggc ggcgctggcg acggcccgtc
     4681 tgctgaagtc gtcggttctg aaggcttcct cggcgccgtt cattctggaa ctgccgcaat
     4741 atcggttgcc caccgtgcgc tcgctgagtt tgcgactggt ggatcgcggc aaggtgtttt
     4801 tgcgccaggc aggaactgtg attctggcgg tgacgctggt tctgtgggtg ctaagccata
     4861 ttccgtttca cacggatctg accacgagcg tgatcggacg cctggggcat tggatcgaac
     4921 cggcgattcg gccgctggga ttcaactgga agatcgggat cgggctgctg acttcggtcg
     4981 tcgcgcgcga ggtcattgtc ggaacgctgg gcacactgta tggggccgat ccggcgacgc
     5041 agtcgctggg gctgcaggcg gctttgcggc atgacctgac gctgggcggc gcgatggcac
     5101 tggtggtatt tttcgcgttc gcgctgcagt gcacctcgac gctggcaatc gtgcggcggg
     5161 agacgaacag ctggaagtgg ccggcgctgc agttcgttta tatggggtgt ctcgcgtatc
     5221 tgatggcgct gctgacgaac caggcgatat tgcactttgt ccgatagcaa aagagcagtt
     5281 agcaaaagtc cggttggcga agaagcggtt cgctaaaaac cggttagcgg cgcatgcaga
     5341 agggcttccc attggggagt cctttttcct tgcggccctg gtgaggaccc gacattacag
     5401 aatccagaag agcgggataa ggccgagcag ggcgagcgaa acgagaaaga tgaagctctg
     5461 gaggccggtg cgatggtgtg caaagctcca ggcgtagatc cctttagcga tgttattgct
     5521 cgaagcggcg atgagtgcgc cagcggccgc ggttttcgcg aggatgagtg tgccggcggc
     5581 ctgggtcatg ccgaggatga aggggtcgac gtcggcaaac cccatgatgg cggcgagggt
     5641 gttgacgccg gttctcccaa cataggtgac ggcgagctgc gtggcgatga gcatggcgag
     5701 gaagatcgcg gcaaagagga aagctgcgcc gagctcgaga gggttggagg gctcgaattc
     5761 gcgctgcacg gattgggcgg tggggtcggc gcggcgtgtc cagagccagc cggtcaggat
     5821 tgcgagtgca gcgaggacga gaaaaggcag atcgaccagg gccatcattt gccggttgaa
     5881 gagagcgagc aggacagcga ggcgaacgta catgacaccg gaggcgatga ggatgccacc
     5941 ggagaagagg tgtggatgct cctcctgttt ggagcggcgc gccatgacga tggtggtcac
     6001 ggtggaggag taggcgccgc cgagcaaagc cgctacgacg acgccaccct gggacttcgt
     6061 gattttttgc aggatgtagc taccgtagga gatggcgctg atggcgacga cgacgagcca
     6121 ggttttgaag ggattgatgt ggaagcgggt gaactcctgg cgcggcagga cggggagaat
     6181 gacgccggtg aggaggagaa acttggcgaa ggtaaggatt tcgcggggag cgacgcggcc
     6241 ggcgagtttt tcaagcgcga ctttgaggtc gagcagaagc aggctggcga cgctgagggt
     6301 ggtggcgatc cagaaatgtc cgtagcagac cagggcgccg acgacgaagg tggtaaggcc
     6361 ggacatttcg gtggtgacgc cggcggcttc cggctgggaa agcttgtgcc agtaggagag
     6421 cagcagaaat cctgcgacaa cgaggaagcc gatcgtcagg ggcagaagct ggttgccgga
     6481 gagcagcgca agcgagtagc cgaggaggcc gatgagggga aaagtgcgga cgccgccgaa
     6541 ggaatatctg ccgggctgag acttgtaatc ttcgcgctcg agaccgatga ggaaggagag
     6601 aaagagaaca aggacgatct tgacggcgtc gggcggaaga tgcgggaagt tttgcacggt
     6661 gagtcacgcc ttcgagccga ttggcttccg gcttgagaaa ctcggatcta atgggtgaac
     6721 ctggtacata agaatacatg ggcacatcgt gtgtgccgtc ggcgaaatgg cagaggcgcg
     6781 agggaccgcg ggtggggacg aaaaatctgg tgagcgccat tcggagtgag atggggaacc
     6841 gcgggaggct acgatggctg agaggcccct gcatggcgaa cagttattcg aagctgtcgc
     6901 tgtatccatt ctttgaaaca ctccgtgccg tgggcagaag tgtgctcgca cttgactacg
     6961 acggaacgct ggcgccgttc cggcagaacc cgatggaagc ggttccatat cccggagtgc
     7021 gtgaagtgat tgagaagatc ctcggcacag ggaagacgcg ggtggtgctg atcagtggac
     7081 ggagggcaga ggaggtgcgc gacctgcttg gagtgcggcc tgcgccggaa atctggggtg
     7141 tgcacgggcg gcagaggttg ggtgcggatg ggcgctccat gatcgcgccg ctggccgagc
     7201 gggaagagga ggcgctggag gctgcggcgg catgggtgga gcattgcggg tacggcgtgc
     7261 gcacagaact aaaaccagga agtcttgcgg tgcactggcg cgggctgacg gcacaggaga
     7321 ttgggagggc gagcgcggcg accgaggcgg cgttgtggcc catcgccgat cgcgccggta
     7381 tgtgcctgct tgggtttgac ggtggggtgg aaattcggac gcgcgaaccg aacaaaggca
     7441 cggtggtgcg agctctgctc ggcgaacaac gggatccgat tccgtttgca tttcttggcg
     7501 acgacaaaac agatgaggat ggatttgcag ttctgcgtgg gacgggtgct ctgagcgtgc
     7561 tggtgcgtcc agagtggcgt gcgacggcgg ctgaagtgtg gctgcgcccg ccggaagagc
     7621 tgctggagtt tctcacggcg tgggcggagt ccggcgaggt gaagcgatga cggtggcggc
     7681 ggggaggcgc gcgaaggccg gccgacgcgc tcgggggcgc aaacgccagg ggactctgat
     7741 tctggtttcg aatcgcctgc cggcgagtct tgaggcggga cccgatggtc cgatcgtaaa
     7801 gcccagtgcc ggcggactgg tgacggcgct gcgcccggtg ctgaaagcgc ggaacggatt
     7861 ctggatcggg tgggcgggga aggggtcgga gagcgagatc gagaaactgg tccgggaggc
     7921 gggtgcggaa ctgggatgcc agatgattcc ggtttcgctg aacgcggcag agcggcaggg
     7981 tttctatgcc ggcttctcga acgagattgt gtggccgctg tttcatgacc tgcagtcgcg
     8041 gtgtaacttc gatccgacgt actggaaggc ttacgtcacc gtcaaccgga agtttgccga
     8101 ggccgtggcg gatgtgacga aaccggattc gatggtgtgg gtccacgact accacctgat
     8161 gttgtgcggg gagttcctgc ggcaacaggg cttgccgagc cgaatgggat tcttcctgca
     8221 cattccattt ccgccactgg atattttcga gaagctgcca tggcgcagtc agattctgcg
     8281 ggcgctgttc cagtacgacg tggtgggttt ccagacgcca cgcgatctgc gcaacttcct
     8341 gggctgcgta cgccggctgt tgcccgatct ggatttccat cgcaccgacg aggatgtgca
     8401 ggcgaccgtg ggcggacacc agacggttct gaagagcttt cccatcggga ttgatttcga
     8461 cgagttttgc gggcaggcgc agcgctcgga agtagtggcg cgcgcggagc agattcggaa
     8521 ggacatgcgc ggagtgcaga tcgtgctcgg ggtggaccgg ctggactaca caaaaggcgt
     8581 gcccgagcgt ctgcgagcag tccggcatct gctggagtca cggcgcggca tgcgtcagaa
     8641 gatcagtctt gtgcagatcg ttgtgccgag ccgcgaggat attcccatgt accgggagct
     8701 gaaactcgag atcgagacgc tggtgagcca gatcaacgga cagttcggcg atccgggctg
     8761 gacgccggtg cactacatgc atcgatcggt ggaccggacg gagttgctgg cgctgtatcg
     8821 ggccgcagat attgcactca ttacgccgct gaaggatggg atgaaccttg tggcaaagga
     8881 attctgcgca gcgcaaaccg gggcgcccgg agttctggtg ctgagcgaat tcgcgggggc
     8941 cgccgaacag ctgcagaatg gcgcgatcct ggtgaatccc tacgattttg ggagtgtggc
     9001 ggaggcagtc aacgaggcgt ttcacatgcc gctggcggag agagcgaagc gtatgcggaa
     9061 gctgcggcgg aaactgcagt atgcagatgt ctttcgttgg tgcgaggagt tctgggaatt
     9121 cctggaaggc tgacccgtgg tcgtgcggag ttcctgggag tgatcaggaa cgagctcggg
     9181 agaaaaagga aaggcttgcc aggtggcgag cctttcgtca ttttgcgctc tattttccgg
     9241 gaaatgtcag ccgcagggaa gatcgctccg ccagtttgag attttggagt ggaagccttt
     9301 ttgtttgttg atcttcaggg gggagatccg cgggggcttg cggagggatg tggcgagtcg
     9361 aggcgaaatg tctgcgccgg gatcaacttt tcttaggtta gcaagggaga gaatcaataa
     9421 aagtaattta catattgaca aaatcaatca ataagcgcat gatgcattca gattgttgag
     9481 aacgggggcg gcgtttgatg gcggaagaag ggaaaggcga cgcagcggaa tggcaggaat
     9541 tgacccggct gacgcagggg cagcctgtgg tggtggagcg ggtgcgactg gtggggaaaa
     9601 acatcgcgat cgaaggttcg tttgagctgc cgcagctggc caggctgaac gccgaggatc
     9661 aggtgtttgt ggtggcgttc ctgcggagcc atggatcgat caaggagatg gagcaggtgt
     9721 tcggggtgag ctatccgacg atcaaggcgc ggctcacgcg gattgcggga agtctggaat
     9781 ttgtggagac ggatcccggt cctccgcggg ctgaggtgct ggagagacta gcgcgcggcg
     9841 agatcgatgc gaccgaggca attcgggcgc tggaagggat gaagggcagg gactaggcaa
     9901 cagggaacag ggagccggtg atgattccga gtttggcggt cgtgcgcgtg gagaatccgg
     9961 attggcggtg gggcggggtg aggctgtggt tgcccctggt cctggtctat atcccgctgc
    10021 tgattttgtc cccgctgatt ttgctggttg ttgtggtggc ttgttggctc gggcgcgtgc
    10081 gtccgtggcg agcgatcgcg gggttctggg aattgttctg cagtttagcg gggaccgatg
    10141 ttcgggtgca ggcggagaag aatcgagtgc tggtcaggat tctgtgaaag cgggccaaag
    10201 ctgacggatg acaggttaca gggagcagag agacggtgat gaagcaggaa cgaaaacaga
    10261 ttctggaaat ggtgatcgcg ggccgcatga cgccgctgga agcggagcgg ttgctgctgg
    10321 tgtggaacga ggagcgcgag agccgctgga tctttggcgc gtgtgcggcg ccggcggtcg
    10381 tgaatcttgc gcatgcggtg tggctacaga tggcgggctc gggtgggata catacggccg
    10441 ccggggtggt gagctggatc cttggaggtg tgcgatgagc gagggacgga agcagattct
    10501 ggagatgctg gcggcgggca agatcaacgc caatgaagca gagcgattgc tggttgcgct
    10561 ggagccaggc agcggaagcg gacccgaggg cggcgcggtc gatgggaaga cgggaaagaa
    10621 gaagccaaag tatctgcgcg tgctggtcga ggccgacgag tcgatgacgg ggctgaaggg
    10681 aggcgcgacc accgtgaacg tgcgcgtgcc gatgcagttg ctgcgggcgg gagtgcggct
    10741 ggcggggctg attccacagc aggcgcacca gcagctcgat caggctctca accgccacgg
    10801 cgtgccgctg acgctgtcgc aaatcaagcc ggaaaacctg gaggagctga tcgatcacct
    10861 cgaggatttg acagtggatg tggatggcaa ggacgggaac acgacgaagg tgaaggtgtt
    10921 ctgcgagtaa acggcggccg ttcgccatta gccgatagcc ggccgccgaa gcggggcacc
    10981 gcaaagaaaa caagctttgg aaggacagac tcctgtttgc agcttcggca ggacaaaggt
    11041 gaagtgcgct cggaagccgg gtgcaggcag aaaaaaccga aaacggagga gaaaaaggat
    11101 gacaaatgta acagcgactg tgacgagaac tatggcgatg ccgaagccgc acagggaccg
    11161 cggtatcgag ggcaggatgg cgcagtggta tgcggcgacg acggcgaagt cgatgggaga
    11221 ttttcgggaa ctggcgcggc ggattgcacg ggaattgccg cagggcggca gcgtgctgga
    11281 gattgcgccg gggccggggt atttctgcat tgagttggcg aagctcgggc cctatgcgat
    11341 tcgcggtgtg gatctgagcg aggcgatggt gaagatagca cgtgcgaagg cagaggaggc
    11401 cggggtgcag gtggatttcg aacggggaaa tgcggcgaac ctgccgtatc cgcgggaagc
    11461 gttcgatttt ctggtgtgcc gggctgcgtt caagaatttc gcgcagccgg tacgggcact
    11521 gcgggagatg tgccgcgtgc tgaaaccggg cgggcgtggc ctgatcatcg atctgcggcg
    11581 gaatgcggga attgcggaag tgaatgagca tgtggacacg atggggttgt cggcgttgaa
    11641 tcgcattatc acgaagctga cgtttcggtg gatgctgttg aagtcggcct atgccaggga
    11701 gcatttcgag cagatgctgg cgaagacggc gttcggggaa tttgaggtga tcgagtcggg
    11761 gattgggctg gaggtgtgga tgacgaagta gagagggccg cgttggttgg aggtctcttc
    11821 gccgggcaga gagagaagcc ggcgcggaga gcggtgggtt ttctcaaaaa cggaacgaga
    11881 gacgtccgtg gtcgttacct ttgggcccgc gcccgtggtc cgtgttctcg tcgaattggt
    11941 gcgccagcgc tttcggggct ggcgttttca ttttttgcag gaatcgcggg ccgcgcgact
    12001 aaagcaggaa tgacaaatca gtgacaactg ggcggccggc gtcgcctatt ctgcgcgaag
    12061 aggccctccc ctccatccct cgttccacgc agccggtgcg acccgtcgca gccgaacgtg
    12121 gaacgagctt ggcgtcgttt caggccagga aagaactcgg tctgccggat gctcgcgtga
    12181 aacgtgccac agcgagcatc cggttttatt gttttcaggt cgcgggtcac cgatcacaat
    12241 cgagaaccga atcgcgcgcc agtggggcac ctgggctgtc cggcggtgcg aggtgctcgt
    12301 catggaagga tcgcgcgccg gcctaacaga atcctgacaa tcgcctgaca gacacatgac
    12361 acggcattgc gaggccgtgg atagcgttgg aacagcgtgg tattgcgatg caattgtcac
    12421 gaacctgatt tttgtcgtga cacggctggt tggaggcctc gaaaggctga aggttgctaa
    12481 ggagacgaga tgaagatgat tctggccgcc agcgcggctg catttgttgc gattgggtct
    12541 gcgccgctga attttgttgc gacgcaggcg gcgccgcagc ggattgaggt aacggcgaag
    12601 cggttctcgt atgaaccgga aaccgtcacg gtgaagaagg gtgagctggt ggagttggtg
    12661 ctgaagagcg aggacgttcc acacgggctg aagttccgtg agttgggtgt ggatgtgaag
    12721 gcgaagaaag gcaagacggg cgaggtggag ttcactccgg aaaagactgg cgatttcgtt
    12781 gggcactgtt cggtgttctg cgggatggga cacggatcga tgcacctgac gctgcacgtg
    12841 gtggagtagg gatgcggcgc gggcgcgaag tgggagtggc agcggtggcg gtcgcggtga
    12901 tgctgggggc ggggtgcaag gcgactccgc caaatgcatt cgagacgcgc gtgatgacga
    12961 tggcaaaaca caaaatgctg gttgggaata aagacgcgaa gaatccgatg gcggcgaatg
    13021 cggagacgat cgctgatgga aagggcgcgt ttgggcacta ctgcgtggcc tgccacggca
    13081 tggatggaca gaacaccggc gttccctttg tcatgtcgcc accggcgccg tccctggcat
    13141 cgcctgacgt gcagagctac acggatgggc aattgaagtg ggtgatcgaa aacgggattt
    13201 ggccttcggg aatgccggga tcgaagggga cgttgagcga cgacgagcaa tggtcgattg
    13261 tgctgttcct gcggcatctg ccaccggtgg gcagcgtggg tgagccggag atgtacacac
    13321 actaggcgcg atggtgagga tacgaagaca ggccggtgtg gtggtgcttg cggcgctcgc
    13381 ggcgggaaac ctcgtgctgc gggcccagag taccgctggc gcagggaaca cagtcaccca
    13441 gtcttcgggg aacggcgcct ggattgatcc agacgataac gccagagctc gagaagatgc
    13501 gctgggccac aatcaggggc cgggctcggg agcaacggca agcgctgggt tggcgctgac
    13561 ggcgcggcag attttcgccg ttctcgatga gaagccggaa gtggtgatcg agctaaagac
    13621 gcagctggcg gagcaactgc agcagcaag
//
LOCUS       DASZYN010000360         1678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_334012, whole genome shotgun sequence.
ACCESSION   DASZYN010000360 DASZYN010000000
VERSION     DASZYN010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334012"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VHE33_09270"
     CDS             <1..193
                     /locus_tag="VHE33_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97685.1"
                     /translation="DQEKALALPVIRRALRSLQPTLRRCGLRAIAQYRQGYVLAPLLP
                     SEDQHIDHTKGRGGYGSRT"
     gene            511..>1678
                     /locus_tag="VHE33_09275"
     CDS             511..>1678
                     /locus_tag="VHE33_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97686.1"
                     /translation="MLWLCSTRERNTCRMAIQPPTTSPGTPPPRAKKARLSNWNMGIA
                     RTHRLTTQLVGMQLSGANLLALTVSAASVVAGTVSLVAQKYPGNWPLLLSAGVIGLGL
                     ALLIEGLTLGSLIRIRLANRAIRLLDEQIEQDQRAAIAALPLPDPALPNARQAMRQYR
                     QTIRLVQQDARRKRQHATSLPRRQRRFSLPFAAMGALCSAAAGGLFYHTILAGLGQME
                     SLALAILFPLVVTGTFVSSELHKDAQEEAIKEGFGGGALTETAIREETKLRSALAVHQ
                     HVLAYLEKPEAEQAIEAGARFLLRDILTELRDTSRQATQQVLFPQGASEPTQQLPPAR
                     QVSVATSPDTTENGETTPAAAATTDNMTPPGVAPDDMTSPDDARGKETTPVAAMP"
BASE COUNT          350 a          551 c          468 g          309 t
ORIGIN      
        1 ggaccaggaa aaagcactcg cgctccctgt gatccgccgc gcactgcgct cgctccagcc
       61 cacgttgcga cgatgtggcc tgcgcgccat cgcccaatat cggcaaggct atgtcctggc
      121 accgcttttg ccatctgaag atcagcacat cgatcacacg aaaggccgtg gcggatatgg
      181 atctcgcacc tgaagccgtg aagcgcctca tgcttctact ccatcagctg acgaagtgtc
      241 tctcatcttc gcgcggttgt cgcaacgggc atcccagcca gaacggatgg acctttcgtc
      301 gcggtctcga tcctggcaag aagcggagcg aaacagatcg atactccagt actcccctgg
      361 tgaaaaacaa gccctggtgc ggcctgccag gctcatcgcg ccgtttatct gagttggaga
      421 accgttcacc agacgagggg catcttttct cctgctctgc accgaacgca cgtcagatcg
      481 tcatcccgga agggtacgcc tatccaggag atgctgtggc tttgtagcac aagagaaagg
      541 aacacgtgtc gcatggcgat ccaaccaccg accacctccc caggaacgcc gccaccgcgc
      601 gcgaaaaaag cccgtctgag caactggaat atggggattg ccaggacaca tcggctgacc
      661 acccaactgg ttggcatgca actctccggg gccaatctgc tggccttaac cgtatcagcg
      721 gcctcggtcg ttgccggaac cgtgtccctg gtcgcccaaa agtatcctgg caactggccc
      781 ctgttgctct ccgccggcgt cattggtctg ggtctggcgc tgttgatcga gggactcacg
      841 ctcggctctc tgatccgcat ccgactcgcc aaccgggcga tccgtctgct cgatgaacag
      901 atcgaacagg accaacgcgc ggcgattgct gcgctgcctt tgcctgatcc ggctcttccc
      961 aacgccagac aggccatgcg ccagtaccgg caaaccattc gtctggttca gcaggatgcc
     1021 cgccgcaagc gccaacacgc gaccagcctg cctcgccgac agcgccggtt ttcactcccg
     1081 tttgccgcga tgggcgcgct ctgtagcgct gcggcaggtg gactgttcta tcacacgatt
     1141 ctggctggat tgggccagat ggaaagcctg gcgctggcga ttctctttcc cctggtcgtc
     1201 accggaacgt ttgtctcctc cgaacttcac aaagatgcgc aagaagaagc gattaaagaa
     1261 ggctttggcg ggggcgcgct gaccgaaacg gcgattcggg aagaaacgaa gttgcgttca
     1321 gcactggcgg tccatcaaca tgtgctggcc tatctggaga agccagaggc ggaacaggcc
     1381 attgaagcag gtgcccgctt cctgctccgg gacatcctca ccgaactccg tgacacctcg
     1441 cgccaggcaa cacaacaggt tctgtttccc cagggcgcca gtgagccgac acaacagctt
     1501 ccacctgcca ggcaggtgag cgtcgcgaca tcccctgaca ccaccgagaa tggggagacg
     1561 acacccgctg ctgccgccac gactgacaac atgacccccc ctggcgttgc gccagatgac
     1621 atgacatccc ctgacgacgc caggggaaaa gagacgacac ctgttgccgc catgccga
//
LOCUS       DASZYN010000361         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_782956, whole genome shotgun sequence.
ACCESSION   DASZYN010000361 DASZYN010000000
VERSION     DASZYN010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782956"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1245)
                     /locus_tag="VHE33_09280"
     CDS             complement(61..1245)
                     /locus_tag="VHE33_09280"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HVT97687.1"
                     /translation="MTTTAASLFSDRIGRIEISATMAVTAEAAKLRAQGAKLVDFGAG
                     EPHFPTPQHIKDAAIEAIQNNFTKYTMVSGIPELRQAIVERHAADFGSNYSPDEAIFS
                     TGGKLAIFNAIQVLIDHGDEVIVPTPYWVSYKDIVQYAGAKCVFVEAGDAEEFRVTAD
                     AIERAITPKTKAILLNSPSNPSGAVIAPKDLERIVHLAKERGIWLLLDECYVYLQYTG
                     ALVSGGSFTDAKENILVLGSLSKTYAMTGWRAGFALGPKAVIAAMSKLQSQSTSSTAS
                     FVQKAAIAALKGDQGCVGTMRADYLKLRDESLAGLAKIPGITCVKPEGAFYVYPNVKQ
                     YLGKVGTAMELSKRLLHEAHVVTVPGEAFGTTEHIRLSYATSHADVAEGLERMRGFFA
                     KL"
     gene            complement(1394..1666)
                     /locus_tag="VHE33_09285"
     CDS             complement(1394..1666)
                     /locus_tag="VHE33_09285"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HVT97688.1"
                     /translation="MSTWKFWAAFGVGVAAGAALALIYAPQSGARTRRQIKRAVNDAG
                     DYIKETADNVQDFAGKYIKRGKDVVDDVVDQAQNVVTSAKRVTTWG"
     gene            complement(1872..>2251)
                     /locus_tag="VHE33_09290"
     CDS             complement(1872..>2251)
                     /locus_tag="VHE33_09290"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF014067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HVT97689.1"
                     /translation="GLPNEKDVKDGMIAYKIAAHAADIARHRPGARDRDDAISYARYT
                     FDWEKQFALSLDPETARAMHDETLPDDYYKEAAFCSMCGPKFCSMNWSSKVDEFNKSV
                     HGLEKQDLSELVTIEAGKLGAKK"
BASE COUNT          383 a          740 c          654 g          474 t
ORIGIN      
        1 cccctgaaga gtgtcttcag gaggtcgcat cgcaagccaa atggggcacc ggctgcagtc
       61 ttatagcttc gcgaagaacc cccgcatccg ctccaacccc tcagccacat cggcatggct
      121 ggtcgcgtac gacaagcgaa tgtgctccgt cgtcccgaac gcctcacccg gcaccgtgac
      181 cacgtgcgcc tcgtgcagca gccgcttcga cagctccatc gccgtgccga cttttccgag
      241 atactgcttc acgttgggat aaacgtagaa cgcgccctcc ggcttgacgc aggtgatgcc
      301 cggaatcttc gcgagaccgg cgagcgactc atcgcgcagc ttcagatagt cggcgcgcat
      361 ggtgcccacg cagccctgat cgcccttgag cgccgcaatc gccgccttct gtacgaagga
      421 tgccgtgctc gacgtcgact ggctctgcag cttcgacatg gccgcgatca ccgccttcgg
      481 tcccagcgcg aaccccgcgc gccagcccgt catcgcataa gttttcgaca gcgacccgag
      541 cacaaggatg ttctccttcg cgtcggtgaa ggatccgccg ctgaccaacg cgcccgtgta
      601 ctgcaggtag acgtagcact cgtcgagcag cagccagatg ccgcgctcct tcgccagatg
      661 cacgatgcgc tcgaggtcct tcggcgcgat cacggcgccc gacgggttcg atggcgagtt
      721 gagcaggatg gccttcgtct tcggcgtgat tgcccgctcg atggcgtccg cggtcacgcg
      781 gaactcctcg gcatcgccgg cctcgacgaa gacgcacttc gcgcccgcat actgcacgat
      841 gtccttgtag gagacccagt agggcgtggg aacgatgacc tcgtccccgt gatcgatgag
      901 cacctggatg gcgttgaaga tggccagctt gccgccggtc gagaaaatgg cctcatcggg
      961 cgagtagttc gagccgaaat cggcggcgtg ccgttcgacg atggcctgac gaagctccgg
     1021 aatgccggag accatggtgt acttcgtgaa gttgttttgg atcgcctcga tcgccgcatc
     1081 cttaatatgc tgcggcgtcg ggaagtgcgg ctcacccgcg ccaaaatcca ccagcttcgc
     1141 gccctgggcg cgcagcttcg cagcttcggc ggtcacagcc atggtggcgg agatttcaat
     1201 ccgcccgatg cggtcggaga aaagtgaggc agcagtggtg gtcatgctgc tattttttca
     1261 gattttcgcg gtcccggcct gccgtttttt cgacagaccg gaggccggag atttcatcgg
     1321 aaaactaacg gccaatcggg cgcgcgtgaa ccgccactat tccctgctgg ctgttctccg
     1381 ctccctgccc ttctcagccc caggtcgtga cgcgcttggc cgaggtcacc acgttctgtg
     1441 cctggtccac cacgtcgtcc accacgtctt tgccgcgctt gatgtatttc ccggcgaagt
     1501 cctgcacgtt atccgccgtc tccttgatgt agtcgcccgc atcgttcacg gcccgcttga
     1561 tctgacgacg cgtgcgcgcc cccgactgcg gcgcgtagat cagcgccagc gccgcgcctg
     1621 ccgcgactcc aacaccaaag gctgcccaaa atttccaggt gctcatgtct tactccttcc
     1681 aggatttatg ccaggtttcc tgctcagtcc gattttgccc tcttgtcggt cagatacggc
     1741 ggactgtctg ttggttgcgt tgattcctcc cctgtggaaa gcaaaagccc caaaagccaa
     1801 aagccggcag agagcaaagc agatttcttt gcggtccttg ccggcttttt gacgttatga
     1861 ccgcaggcac gtcacttctt tgctcccagt ttgccggcct caatggtaac cagctcgctc
     1921 aggtcctgct tctcgaggcc gtgcacgctc ttgttgaact catccacctt gctcgaccag
     1981 ttcatggagc agaatttcgg cccgcacatc gagcagaagg ccgcttcctt gtagtagtcg
     2041 tccggcagcg tctcatcgtg catcgcgcgg gcggtttccg ggtcgagcga gagcgcaaac
     2101 tgcttctccc agtcgaaggt gtagcgtgcg tagctgatgg cgtcgtcgcg gtcgcgcgct
     2161 ccgggccgat gccgggcaat gtccgccgcg tgggcggcaa tcttgtaggc gatcatgccg
     2221 tccttcacgt ccttctcgtt gggcaggccc a
//
LOCUS       DASZYN010000362         4988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1040513, whole genome shotgun sequence.
ACCESSION   DASZYN010000362 DASZYN010000000
VERSION     DASZYN010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040513"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VHE33_09295"
     CDS             <1..325
                     /locus_tag="VHE33_09295"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HVT97690.1"
                     /translation="SNIADYHMNIQAALSEPRFTVSSKLGCTIVIESRVGQATIDQLT
                     KMGHELQVRKEYTTAMGRGQAVLHNDATGINYGASDPRADGAAIPETPKFPEPTAERV
                     AKSNP"
     gene            complement(461..2095)
                     /gene="rmuC"
                     /locus_tag="VHE33_09300"
     CDS             complement(461..2095)
                     /gene="rmuC"
                     /locus_tag="VHE33_09300"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HVT97691.1"
                     /translation="MLLILGLSALADSQLLLILGLQAAILLGVLLLMLRKNPIGPISA
                     LLQMLGERMAEMNARSEHVETSVHSIRTDLTTGLGQSRTDESRFFDQVRKESTELREL
                     VTTRLETIGKQAGERHIALVESIQARLTAIGETLASGQMTQSAETLRMKETVETTLSQ
                     LGRDLRENTNQLTEEVKGRLKDVAEQMTTLSEANERRQETLRQTVDGRLESLQQSNTS
                     KLEEMRATVDEKLHATLERRLTESFSMVAERLENVQSGLGEMKDLAVGVGDLKRVLTN
                     VRARGSMGETQCGAQLDQILSPDQYIRNATIKEGSRECVEFAIRIPNGDQPEMLLPID
                     VKFPKEDWERLEEALDHGAADEIAAARKSLASTVRNEAKKICDKYIDPPRTTPFALMY
                     VPTEGLFAEIIRVPGLIDELQTHFHVSVAGPTNFVAILNSLQMGFRTVAIQKKGAEVW
                     RVLGAAKMEFQKFGKLMAKVESQVSTVQNTLKDISSKTRTINRTLKDVSTLETQPAEV
                     SGLLGIHEQQALPLTPDQGQGLIVELAAEEEQVSES"
     gene            complement(2215..2508)
                     /locus_tag="VHE33_09305"
     CDS             complement(2215..2508)
                     /locus_tag="VHE33_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97692.1"
                     /translation="MPSKAKVHSILEGRQRRDPQSPTQQQDQKQSPAQKSATSVNPTE
                     PADKKATGTSGALQNEILRHNFRGGQAQPHPATPAGQHATGSFPTGVPKNSRK"
     gene            complement(2585..3160)
                     /locus_tag="VHE33_09310"
     CDS             complement(2585..3160)
                     /locus_tag="VHE33_09310"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HVT97693.1"
                     /translation="MDYLWTPWRYTYVTEGDKKKRAGIPEELSAWPGDLHCVFCNMIA
                     AVDYVVEHGMPRREAERAAGILLRAERNYICVNRFPYTSGHIMVVPYEHQASLAALPQ
                     ETAHEMIRLAQRAERALTGLYHPDGFNFGLNLGKSAGAGVAGHVHLHALPRWTGDTNF
                     MTTVGETRMLPEDFFVTWERVRGALETDAKP"
     gene            complement(3204..4682)
                     /locus_tag="VHE33_09315"
     CDS             complement(3204..4682)
                     /locus_tag="VHE33_09315"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HVT97694.1"
                     /translation="MARLFATKPLSVIRQEAAEEGEHTLKRSLGAVNLITLGIGAIIG
                     AGIFVLTGTAAAQFAGPAIVLSFVVAGIGCVFAGLCYAEFASMIPIAGSAYTYGYTTL
                     GEMMAWIIGWALCLEYAFGAATVAVGWSGYVSSLLGYFGIKMPFNPTPSLALNLFGHP
                     LSAKVDIFALCAIIVVTTILVVGVKESANFNSAAVMIKVSVVLFFVGIATIFAFTHWS
                     HMMANWHPFIPKNQGSFGAYGWSGIMRGGSVVFFAYIGFDAVSTAAQEARNPQKDLPF
                     GILGSLIICTVLYIIVSALLTGIKPYTTLNTPSPVADGAAAIGATWGVVLIDLGAIAG
                     LASVMLVMILGQTRVLYTMAHDGLLPKWVGSIHPRFRTPWLVTIATGVLVAFLAAFFN
                     ISFLGELVSLGTLLAFTIVCLGVIVMRRKQPDVPRPFRTPWVPFVPIMGMIISLALMV
                     SSDTPAKIGFFSWLTVGLLIYFSYSVRHSKVQAMNPEVTSGR"
     gene            complement(4675..>4988)
                     /locus_tag="VHE33_09320"
     CDS             complement(4675..>4988)
                     /locus_tag="VHE33_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97695.1"
                     /translation="LDYIICTELIFYILTIACLYVLRAKRPNAERPYRAIGYPVLPAV
                     YILMAAWICIVLLRYKPQYTWPGLVIALLGVPVFLLWSRNAPRAESSPETISGGTAEN
                     G"
BASE COUNT          909 a         1398 c         1707 g          974 t
ORIGIN      
        1 ttcgaacatt gccgattacc acatgaacat ccaggcggcg ctttctgagc cccggtttac
       61 ggtcagcagc aagctgggtt gcaccatcgt gattgagtcg cgcgtggggc aggcgacgat
      121 cgatcagctg acgaagatgg ggcacgagct gcaggttcgc aaggagtaca cgacggccat
      181 gggccgcggg caggcggtgc tgcacaacga cgcaaccggg atcaattacg gggcgtcgga
      241 tccgagggcc gatggggcgg caattccgga gacgccgaag ttcccggagc cgacagcgga
      301 gcgggtggcg aagtcgaatc cctgagcggc ggctgaagcc ggcttcattc tgcgccgtta
      361 ttggcacagc tgaagcagtg tcctaccagg gcaacgtgtc tttccgcagg ctgtgaagcc
      421 gtgccttttc aaaaccggta acaaactctt cgacaggcca ttagctctcg gagacctgct
      481 cttcttcggc ggccaactcg acgatgagcc cctggccctg atcgggagtc agcggcaggg
      541 cctgctgttc gtggatgccg agcaggccgg acacttcagc cggctgcgtt tcgagcgtgc
      601 tgacgtcctt cagagtgcgg ttgatggtac gcgtcttgct gctgatgtcc ttgagggtat
      661 tttgcacggt cgaaacctgg ctttcgacct ttgccatcag cttgccgaat ttctggaatt
      721 ccattttcgc cgcgcccaga acgcgccata cttcggcgcc cttcttctgg atggcgacgg
      781 tgcggaagcc catctgcaga ctgttgagga tcgcgacaaa gttggtgggt ccggcgaccg
      841 acacgtggaa atgtgtctgc agttcgtcga tgagtccggg cacgcgaatg atttcggcaa
      901 agagaccttc cgttggcacg tacatcagcg cgaagggcgt agtccgaggc ggatcgatgt
      961 acttgtcgca aatcttcttt gcttcgttgc ggacggttga tgcgagcgat ttgcgcgctg
     1021 ccgcaatctc atcggcggct ccgtgatcca gcgcttcttc cagacgctcc cagtcttctt
     1081 tagggaattt cacgtcgatg gggagcagca tctcgggctg atcgccattg ggaatgcgga
     1141 tagcgaactc gacgcattcg cggctgcctt ccttgatggt cgcgttgcgg atgtactgat
     1201 cgggcgagag gatttgatcg agctgggcgc cgcactgggt ttcacccatg ctgccgcggg
     1261 cgcggacgtt ggtgagcacg cgcttgagat cgccgacgcc gacggcgagg tctttcatct
     1321 cgccgaggcc ggactgaaca ttttcgagac gctcggccac catgctgaag ctttcggtca
     1381 gacggcgctc gagggtggcg tgcagtttct cgtccaccgt cgcgcgcatc tcctcgagct
     1441 tcgacgtatt gctttgctgc agcgattcca ggcgcccatc gacggtttgg cgcagagttt
     1501 cctggcggcg ctcgttggct tcggagagcg tggtcatctg ctcggctacg tccttcagcc
     1561 ggcctttcac ttcttcggtc agctggttcg tgttctcacg cagatcgcgt ccgagctggc
     1621 tcagggtggt ctcgacggtc tctttcatcc tgagcgtctc agcggactgg gtcatctgac
     1681 cggaggcgag ggtttcgccg atggctgtca gcctggcctg aatgctttcg accagagcga
     1741 tatgccgttc gccggcctgt ttcccgatgg tttcgagtcg tgtggtgacg agctcgcgca
     1801 gctcggtcga ttctttgcgc acctggtcga agaatcgcga ttcatccgtg cgtgactggc
     1861 ccagtccggt ggtgagatcg gtccggatgc tgtggacgct ggtctccacg tgctcggagc
     1921 gggcattcat ttccgccatg cgctccccca gcatctgcag gagagcagag atgggcccga
     1981 tagggttttt gcgaagcatc aggaggagga cgccgaggag aatcgccgcc tgcaagccga
     2041 ggatgaggag cagctgcgaa tcagccagcg cagagaggcc gaggatgagg agcattgggg
     2101 gttctccaag agcagaatac cccgtcaccg cgcgggctcc ggccaaattt cacagccggc
     2161 ccctcaccgc gcggaggcgg cggaagccag ctgtgggcga tgcgtcggtt cagtttactt
     2221 tctgctgttc ttcgggacgc cggtgggaaa cgatccggtg gcatgctgtc cggcaggcgt
     2281 tgccggatgc ggctgggcct ggccgcccct gaagttatga cggaggatct cgttttgcag
     2341 tgcgccggag gttccggtcg ccttcttgtc cgccggctct gtcggattca cgctggtggc
     2401 ggatttctgg gctggtgact gcttctgatc ctgttgctgc gtcggggact gagggtcgcg
     2461 ccgttggcgc ccctccagaa tggagtgaac tttggctttt gacggcatct tggggccctc
     2521 ccggaagccc gatggttggg gctctcgctc caggatgaga tgcgcggcga tggcgaatcc
     2581 gccgtcaggg cttcgcgtcg gtttccagag ctcctcgcac ccgctcccag gtgacgaaga
     2641 aatcctcggg caacatgcgg gtttcgccga ccgtcgtcat gaagttcgtg tcgccggtcc
     2701 agcgcggaag ggcgtggagg tggacgtgtc cggccacgcc ggcgccagcg gatttgccga
     2761 ggttgaggcc aaagttgaag ccgtcgggat ggtaaaggcc ggtcagggcg cgctccgcgc
     2821 gctgggcgag gcgaatcatt tcgtgcgccg tttcctgggg cagcgcggcg agggatgcct
     2881 gatgctcgta gggaaccacc atgatgtggc ccgaggtata gggaaagcgg ttgacgcaga
     2941 tgtagtttcg ctcggcgcgc aggaggatgc cggcggcacg ctcggcttcg cgccggggca
     3001 tgccgtgttc gacgacatag tcgacggcgg cgatcatatt gcagaagacg caatgcaggt
     3061 cgccgggcca ggcgctcagc tcttcgggaa tcccggctcg ctttttctta tcgccttcgg
     3121 tgacgtaggt gtagcgccag ggggtccaga ggtagtccac ccgcgcagta taacgtcagg
     3181 ccctcactgc ccgatgatgg cgattatcgg cccgaggtca cttccggatt cattgcctgc
     3241 accttgctgt ggcggaccga gtagctgaag tagatgagca ggcccacggt cagccagctg
     3301 aagaagccga tcttcgcggg cgtatccgac gagaccatca gggcgaggga gatgatcatg
     3361 cccatgatgg gcacgaacgg cacccagggc gtgcgaaagg gccgggggac atcgggctgc
     3421 ttacggcgca tgactatcac gccgaggcag acgatggtga aggccaggag cgtgccgagg
     3481 ctgaccagct cgccgaggaa agagatgttg aagaaggcgg cgaggaaggc gacgagcacg
     3541 ccggttgcaa tcgtgaccaa ccagggcgtg cggaagcgcg gatggatgct gcctacccat
     3601 ttgggcagca gtccgtcgtg ggccatggtg tagagcacgc gggtctggcc gaggatcatg
     3661 acgagcatga cggaggcgag accggcgatg gcgccgagat cgatgaggac gacaccccac
     3721 gtggctccaa tcgcggccgc accgtcggcc accggcgaag gcgtgttgag ggtggtgtac
     3781 ggcttgatgc cggtcagcag cgcgctcacg atgatgtaga ggacggtgca gatgatcagc
     3841 gagccaagga tgccgaaggg gagatccttc tgcggattgc gcgcctcctg ggcggcggtg
     3901 gagactgcgt cgaagccgat gtaggcgaag aacacgacgc tgccgccgcg catgatcccc
     3961 gaccatccgt acgccccgaa gcttccctga tttttcggaa tgaagggatg ccagttggcc
     4021 atcatgtgcg accagtgggt gaaggcgaag atggtggcga tgccgacaaa gaacagcacc
     4081 acggaaacct tgatcatgac ggcggcgctg ttgaagttgg ccgattcttt cacgccgacg
     4141 accaggatgg tcgtgaccac gatgatggcg cagagggcga agatgtccac cttcgccgag
     4201 agcgggtggc caaagaggtt cagggccagc gacggggtgg gattgaaggg cattttgatt
     4261 ccgaaatagc cgagcaggct gctgacgtag ccgctccagc cgacggccac ggtggcggcg
     4321 ccaaaggcgt attcgaggca gagggcccag ccgatgatcc aggccatcat ctcgcccagg
     4381 gtggtgtagc cgtaggtgta ggcgctgccc gcgatgggaa tcatggaggc gaactcggcg
     4441 tagcacaagc ctgcgaagac gcatccgatg ccggcgacca caaaggacag cacgatggcc
     4501 ggaccggcaa actgggctgc cgccgttccg gtaagcacga agatgcctgc gccgataatg
     4561 gcgccgatgc cgagcgtgat caggttgacg gcgccgagcg agcgtttgag cgtgtgctcg
     4621 ccttcttcgg cggcctcctg gcggatgacg gaaagcggct tggttgcaaa aagtctagcc
     4681 attctcggct gttcctccgg aaatcgtttc aggcgagctt tctgcccgag gtgcgttgcg
     4741 agaccaaagc aggaaaaccg ggacgcccag cagagcgatc accagtcccg gccatgtgta
     4801 ctgcggtttg tagcgcaata gtacgataca aatccacgcg gccatcaata tatagacggc
     4861 aggcagcacg ggatagccga tggcgcgata aggccgctcg gcgttggggc gcttcgcgcg
     4921 cagcacgtag aggcacgcga tggtgaggat gtagaagatc agctccgtgc agatgatgta
     4981 gtcgagca
//
LOCUS       DASZYN010000363         3936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1040520, whole genome shotgun sequence.
ACCESSION   DASZYN010000363 DASZYN010000000
VERSION     DASZYN010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040520"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..1162)
                     /gene="dapA"
                     /locus_tag="VHE33_09325"
     CDS             complement(239..1162)
                     /gene="dapA"
                     /locus_tag="VHE33_09325"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HVT97696.1"
                     /translation="MNLQGCGTALVTPFRADGSIDERALYDLARWQVESGIDWLVACG
                     TTAETPTLDEGEWLEVIRIVADAAQGRVPVWAGCSHNSTREAIARTRTASQIPGVTAI
                     LSANPWYNKPTQEGQFQHFRAVAQAVNLPIVLYNIPGRSAANLEPATVLRLIEAAPNI
                     EAVKESSGNLVQITELITRAPKEFRVFSGDDGMTLGVIGVGGAGLVSVASNIIPAEMA
                     EMVKAALANEWSAARRINKKYFRLMQGLFKETSPGPAKAILAMMGKLEEHYRLPMVPV
                     SDGTRAFLERMAGELGLLVQAPQSEADLRMF"
     gene            1207..1776
                     /locus_tag="VHE33_09330"
     CDS             1207..1776
                     /locus_tag="VHE33_09330"
                     /inference="COORDINATES: protein motif:HMM:NF007797.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid biosynthesis
                     acetyltransferase"
                     /protein_id="HVT97697.1"
                     /translation="MATPVQDLKSFRLPHNFRGRPAWFVQLWWLVQATAFRWSPQFMY
                     GWRNFLLRLFGAKIGDGVIIRPNAEITYPWKLTIGDHCWIGDHVGLYTLGEIRIGDNA
                     CISQHCYLASAHHDYTRPTFDMIGSFVAIEPEVWLATRVFVGPGVTVGRGAVVGACSV
                     VLKDLPPMMVCAGNPAKPIRPRPTASDNV"
     gene            complement(1847..2527)
                     /locus_tag="VHE33_09335"
     CDS             complement(1847..2527)
                     /locus_tag="VHE33_09335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HVT97698.1"
                     /translation="MLFMVLGRGKTGSLVAKVAHERGHGVRVVGEEENRNAGALTAPV
                     LAGFDAVIDFTTPEAAVQNMRACLATGARMVVGTTGWYQHLDDMKALAQRRGGALLYS
                     SNFSVGVQALFKVTRDLAAAAHGYRFSITETHHATKKDAPSGTALSLKRIIEGTSPGT
                     TVEIASKREGDVPGIHEVEARSDYDVLTLKHEAHSRQGFAEGAVRAAEWIAEKTGCWD
                     FEEIVGQL"
     gene            complement(2530..>3936)
                     /gene="lysC"
                     /locus_tag="VHE33_09340"
     CDS             complement(2530..>3936)
                     /gene="lysC"
                     /locus_tag="VHE33_09340"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006570.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HVT97699.1"
                     /translation="EDAVAIRRTAGIVAGRVAKGLQPIVVVSAMAKVTDLLLSAAATA
                     AKGDRASALAITERLRIRHADTATQLAFGAKLHDDPLVATQKDEALSATHAWMETEFD
                     GLDEILRGLAVVGELTPRIGDMIVSYGERLSSRMITEAFVRCGINAVHVDARQSIVTD
                     AQYGKAIPQDTLIEQRLKQNVVPLLAEGRVPVMGGFIGSTEQGVTTTLGRGGSDFTAA
                     LVGGGIDAGAIEIWTDVNGIMTADPRVCPDALRVKTISFEEAAELAYFGAKVLHPATI
                     LPAVKKNIPVWVLNSKNPENGGTKITAMPAHCKSPFKSIAAKKRLTIMDIVASRMLLS
                     HGYLRAIFEVFDKHKCPVDMVSTSEVSVSLSVDSNEKLPAIAADLSKLADVKYEGRKA
                     LICMVGENIRGHNGIAARVFQTVRHVNLRMISQGASEINMSFMVEEDDADEAVRSLHA
                     EFFAEADPTVFDLEAAVK"
BASE COUNT          693 a         1364 c         1115 g          764 t
ORIGIN      
        1 cgcccggtgg cgcgccgcgg cagaatcacg gtctcaaagg cgtcgagcag agcagacacc
       61 agacacgcga tgccgagaat cagccgaaga gggtgcactt cgccagcaga gcaggaatcg
      121 atagccgagt caatggacca caaccaattg caaaagagtt ttggggctga ggtcggcgtg
      181 ggtggccaac acgctgcgaa agggcgtgac gctttgcagc caggcaggcc aaacgcgatc
      241 agaacatgcg caggtcggct tcggactggg gtgcttgcac cagcaggccc agttctccgg
      301 ccatgcgctc gaggaaggcc cgcgtcccgt ccgacaccgg caccatgggg agccggtagt
      361 gctcttcgag tttgcccatc atggccagaa tcgctttcgc cggaccgggg ctcgtctctt
      421 tgaacaaccc ctgcatcaga cggaaatact tcttgttgat gcgacgggcc gccgaccact
      481 cgttcgccag cgccgccttc accatctccg ccatctcggc cgggataatg ttcgaggcga
      541 cggataccag gcccgcgccg cctacgccaa tcacgcccag cgtcatgccg tcatcgccgg
      601 agaagacgcg aaactccttc ggcgcccgag tgatcaattc cgtgatctgc accagattgc
      661 cgctggattc cttcactgcc tcaatattcg gcgcggcttc gatcagccgc aacacggttg
      721 ccggctccag gttcgccgcc gaccggccgg gaatgttgta gagcacgatg ggcagattca
      781 cggcctgggc aacggcgcga aaatgctgga actgcccttc ctgggtgggc ttgttgtacc
      841 aggggttcgc cgacaggatc gccgttacgc ccggaatctg cgatgccgtc cgcgttcgcg
      901 cgatggcctc cctcgtcgag ttgtgggagc agccggccca caccggcacg cgcccctgcg
      961 ccgcatcggc aacgatgcgg atgacctcga gccattcgcc ttcatccagc gtgggggttt
     1021 cagcggtggt cccgcaggcg accagccagt cgatcccgct ctccacctgc cagcgggcca
     1081 ggtcgtacag cgcccgctca tcgatgctac cgtccgcgcg aaacggcgtg accagtgctg
     1141 ttccgcagcc ctgcaggttc ataacagacg agtctacacc gccttcgatg ctctacactg
     1201 cacacgatgg caacgccggt tcaggatctg aagagctttc gcctgcctca caattttcgc
     1261 ggccgcccgg cctggttcgt gcagctgtgg tggctcgtcc aggcgacagc cttccgctgg
     1321 tcgccgcagt tcatgtatgg atggcgcaat tttctgctgc gcctgtttgg agcaaaaatt
     1381 ggcgatggcg tcatcatccg gcccaacgcc gaaatcacgt atccctggaa gctcaccatc
     1441 ggcgaccact gctggatcgg cgaccacgtt gggctctaca cactgggcga aattcgcatc
     1501 ggcgacaatg cctgcatctc gcagcactgt tacctggcat cggcccacca cgactacacc
     1561 aggcccacct tcgacatgat cggcagcttt gtcgccatcg agccggaagt gtggctggcc
     1621 acccgcgttt ttgtcggtcc cggcgtcacg gtgggtcgcg gtgccgttgt cggtgcctgc
     1681 agtgttgtgc tcaaagattt gccgccgatg atggtctgcg ccggcaatcc cgcaaaaccg
     1741 atccgcccac gtccgacagc gtcggacaat gtctgacgcg cgtccgacat taacggtccg
     1801 atcgtcttac acagccattt cgttgcgcac cacttcctgg ctgctgctat agctgcccaa
     1861 caatctcttc gaaatcccag cagcccgtct tctccgcgat ccactccgct gcccggaccg
     1921 cgccctcggc aaatccttgc cgtgaatgcg cttcatgttt cagcgttagc acgtcgtagt
     1981 ccgaccgcgc ctcgacctcg tgaatccccg gcacatcgcc ctcgcgcttc gaggcaatct
     2041 ccaccgtcgt tcccgggctc gtgccttcga tgattctctt cagcgacagc gcggttcccg
     2101 agggcgcatc cttctttgtc gcgtgatggg tctctgtgat gctgaaccgg tagccgtggg
     2161 ccgcggctgc caggtcgcgt gtcaccttaa acaacgcctg caccccaacg gaaaaattcg
     2221 aactatagag cagcgctccg ccccggcgct gagccagagc cttcatgtcg tccaggtgct
     2281 gataccagcc cgtggttccc accaccatcc gagccccggt cgcgaggcag gcgcgcatat
     2341 tctgcaccgc cgcctcgggc gtggtgaagt cgatgactgc gtcgaatccg gccagcacgg
     2401 gagcggtcag agccccggcg ttgcgattct cctcttctcc gaccacgcgc acgccatgtc
     2461 cgcgctcatg cgccaccttc gccaccaggc tgcccgtctt tccgcgcccc agaaccatga
     2521 acagcatcgc tacttcaccg ccgcttcgag atcgaagacc gtggggtccg cttccgcgaa
     2581 gaactccgcg tgcagcgacc gcaccgcctc atccgcatca tcttcttcca ccatgaagct
     2641 catgttgatt tccgaggctc cctgcgaaat catcctcagg ttcacgtgcc gcacagtctg
     2701 gaacacacgc gctgcgatgc cgttgtgccc acgaatattc tcgcccacca tgcagatgag
     2761 tgccttgcgc ccttcatact tcacatcggc gagcttgctc aggtccgcgg caatcgccgg
     2821 cagcttctca ttcgagtcca cgctgagcga gacgctgacc tcggacgtcg acaccatatc
     2881 caccgggcac ttgtgtttgt cgaatacctc gaaaatcgcc cgcagatagc cgtgcgacag
     2941 cagcatgcga ctggcgacga tgtccataat cgtgagccgc ttcttggccg cgatggactt
     3001 gaatggactc ttgcagtgcg cgggcatcgc ggtgattttc gtcccgccgt tctccggatt
     3061 tttcgagttc agcacccaca ccgggatgtt cttcttcacc gccggcaaaa tcgtcgccgg
     3121 gtgcaggacc ttcgcgccga agtaggccaa ctctgcggcc tcttcaaaac tgatcgtctt
     3181 cacccgcagt gcatccgggc acacgcgcgg atcggcggtc atgatgccgt tcacgtcggt
     3241 ccagatttcg atcgcaccgg catcaatgcc gccgcccacc agagccgccg tgaaatccga
     3301 cccgccgcgc cccagcgttg tggtcacacc ctgctcggtc gagccgatga acccgcccat
     3361 cacgggcacg cgcccctcgg ccagcagcgg aaccacgttc tgcttcagcc tctgctcgat
     3421 cagcgtgtcc tgcggaatcg ctttgccgta ctgcgcgtcg gtcacgatcg actgccgcgc
     3481 gtctacgtgc accgcattga tgccacagcg cacgaaggcc tcggtgatca tccggctcga
     3541 gagccgttcg ccgtacgaca cgatcatgtc gccgatgcgc ggcgtcagtt cgcccacgac
     3601 cgccaagccg cgcaggattt cgtccagccc gtcgaactct gtctccatcc aggcatgggt
     3661 tgcagaaagg gcttcgtctt tctgggttgc gacgagcgga tcgtcgtgca gcttcgcgcc
     3721 aaacgccaat tgcgtggcgg tgtcggcgtg ccgaatacgc aatcgctcag tgatcgcgag
     3781 cgcactcgcg cggtcccctt tggctgccgt cgccgccgca gaaagcagca agtcggtcac
     3841 cttcgccatc gcgctcacaa ctacgattgg ctgcagcccc ttcgcgacgc ggccggcaac
     3901 gatgcccgcc gtgcgccgga tcgcgaccgc gtcctc
//
LOCUS       DASZYN010000364         3555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_593303, whole genome shotgun sequence.
ACCESSION   DASZYN010000364 DASZYN010000000
VERSION     DASZYN010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593303"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VHE33_09345"
     CDS             <1..203
                     /locus_tag="VHE33_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97700.1"
                     /translation="AEPIVSTETGNFAFKAHPVSEEVRFAIPERRGIRKFDEVTVLFY
                     PETERATLGARVGIEEFELMPK"
     gene            complement(260..2212)
                     /locus_tag="VHE33_09350"
     CDS             complement(260..2212)
                     /locus_tag="VHE33_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97701.1"
                     /translation="MDRRLCSIQATRFFHAINSPAGPLNCPPSRNLRNENIAFRDLCV
                     QPPSPGLSLTTETRIQVLNGKKARSVTPQSADTTSPPTHRRRHHRLVVALLWVGSILA
                     ILAIGALIAGNIFLHRAGPMLKAKVIETLSNRFDSRVELAQFHASFADGFHVSGEGLK
                     LYPNHLDGNEPLFAIKKFSFRVFDWRQILHTPLTVDRVQVSGLSIHLPPKDQRSNMPH
                     VGGHGNGKIDILVRVIDVDQADLVIENGKPGKVPLDFVIQKLELRSVGAGRPMRFHAT
                     LINPKPIGNIDSSGDFGPFDTDSPGDTPVDGTYSFSHADLGTIKGIGGILSSDGSYQG
                     QLDHIVVDGKTTTPDFSLDIAHRPVPLNTKFHAIVDGTNGDTYLQPVDAWLQNTHIVA
                     KGAVVRVQGVKGRDIHLDVTIDPGHIEDVLHLALKDPNPIMTGQLQLHTKFDLPPGPA
                     SVTDKLNLKGSFALSDTRFTSDKIQSKVDELSLRGQGKAKQADQEGQSMKDEAQNKGD
                     SKGDDSKDNSGKDQDQNKSSAGQNQADLANIASNMRGNFTFGNARITLPAINFRVPGA
                     DIALHGVYGIDNQSLDFTGLARLDAHISQMVTGWKSWLLKPVDPFFAKDGAGTQVPIK
                     VGGSTQHPDIGLDFGHHDKDKNKEGK"
     gene            2244..3194
                     /locus_tag="VHE33_09355"
     CDS             2244..3194
                     /locus_tag="VHE33_09355"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HVT97702.1"
                     /translation="MGNGKTGQKIVVGLGELLWDVLPEGARLGGAPANFAVMAGRLGN
                     RAVVASRVGTDALGKQAREELAELPAESDFLQSDSEYATGTVTIRLEHGEPEFTIHEP
                     VAWDRLAMTPEWRDLAQQADAVCFGTLAQRAPVSQATILAFLDETRRDCVRVFDVNLR
                     PPHWSAESLRGGMARATILKLNAGELRHVLMGTGVCPHPVEKADEDEVLRGARRLLDR
                     YPVEMVCITLGAKGSLLVTRHEHHRHPGMTATVKDTVGAGDAFTAALTHYYLEGAPLP
                     LLNEAGNRWGAWVASQSGGMPPLRAETLERVSAEIGARRA"
     gene            3206..>3555
                     /locus_tag="VHE33_09360"
     CDS             3206..>3555
                     /locus_tag="VHE33_09360"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HVT97703.1"
                     /translation="MAHIPSSKERRALGQARRRQVRRQDHARWDAKLRQHDPVALIAE
                     SMRGRVPGLVGLKYERMLASPFGFFRGAVPVMAADLALLPHTGIVNQICGDAHVRNLG
                     AFAAPDGRLLFDIN"
BASE COUNT          621 a          927 c         1320 g          687 t
ORIGIN      
        1 aggcggagcc catcgtgtcg acggagaccg ggaacttcgc attcaaggcg catccggtga
       61 gcgaagaggt gcggttcgcg attccggagc gacgcgggat tcgcaagttc gatgaggtga
      121 cggtgctgtt ctatccggag acggagcggg cgacgctggg cgcgcgagtg ggcatcgagg
      181 agtttgagct gatgccgaaa tagaagagcg gcgagcacag agcgcccagc accgctgctg
      241 gggtgctcgg ctgttgttgc tacttacctt ctttgttctt gtccttgtcg tggtggccga
      301 aatcgaggcc gatgtccgga tgctgcgtgg agcccccgac cttgatgggg acctgggtgc
      361 ccgcgccatc cttcgcaaaa aacggatcga cgggcttcag cagccaggat ttccatcccg
      421 tcaccatctg cgagatgtga gcgtcgaggc gggccaggcc ggtgaaatca agagactggt
      481 tgtcgatccc atacaccccg tgcagggcaa tgtccgctcc cggaacgcgg aagttgatcg
      541 caggcagtgt gatcctggcg ttgccgaacg tgaagttgcc gcgcatgttc gaagcgatgt
      601 tggcgagatc ggcctggttc tggcctgcgg aggacttgtt ctggtcctga tccttgccag
      661 agttatcttt cgaatcgtcg ccttttgaat cgcccttgtt ctgggcttcg tccttcatcg
      721 actggccttc ctgatcggcc tgtttggcct tgccctggcc gcgaaggctc agttcatcga
      781 ctttgctctg gattttgtca ctggtgaagc gggtatcgct gagcgcgaag gagcccttca
      841 ggttgagctt gtcggtgacc gaggcaggtc cgggcggcag gtcgaatttc gtgtggagct
      901 ggagctggcc cgtcatgatg gggttcgggt ctttgagggc gaggtgcaga acgtcttcga
      961 tatggccggg gtcgatcgtg acgtcgaggt gaatatcgcg tcctttgacg ccctgcacgc
     1021 ggacgaccgc gcccttcgca acgatatgcg tgttttgaag ccaggcatcg accggctgca
     1081 ggtaggtgtc gccgttggtg ccatccacga tggcgtgaaa cttcgtgttg aggggaaccg
     1141 gacgatgggc aatgtcgagg gagaagtccg gcgtggtggt tttgccgtcc acaacgatgt
     1201 gatcgagctg gccctggtag gagccgtcgg aagacaggat gccgccgatg cccttgatgg
     1261 tgccgaggtc ggcgtgactg aaggagtagg tgccgtccac cggggtgtcg ccggggctgt
     1321 ccgtatcaaa gggaccgaag tcgccggagg agtcgatgtt gccgatcggc tttgggttga
     1381 tgagggtggc gtggaagcgc atcggcctcc cagcgccgac cgagcggagc tcgagctttt
     1441 ggatgacgaa gtcgagggga accttgcccg gcttgccgtt ttcgatgacc aggtcggcct
     1501 ggtcgacgtc gatgacgcgg acgagaatgt cgatcttccc gttgccatga ccgccgacgt
     1561 gcggcatgtt ggagcgctgg tctttgggag gcagatggat gctcagaccg ctgacctgaa
     1621 cgcgatcgac ggtgagcggc gtgtgcagga tctgacgcca gtcgaacacg cggaaagaaa
     1681 acttcttgat ggcaaagaga ggctcgttgc cgtcgaggtg gttcggataa agcttgaggc
     1741 cctcgccgga gacgtgaaag ccatcggcaa atgaggcgtg gaactgagcc agctccacgc
     1801 ggctgtcgaa gcggttgctg agggtctcga tcactttggc cttgagcatc gggccggcgc
     1861 gatggaggaa gatgttgccc gcgatcaaag cgccaatggc gaggatggcg aggatcgatc
     1921 cgacccacag cagggcgaca accagacggt gatggcggcg gcggtgggtg ggcggagacg
     1981 tagtgtcggc ggattgcggg gtcacgctgc gggccttctt tccattgagg acctggatgc
     2041 gggtctctgt ggttagagac aggccagggc tggggggctg tacgcaaaga tcgcggaagg
     2101 cgatattctc gttgcgcaag tttctggagg gcgggcagtt gaggggtccg gcggggctgt
     2161 tgatggcatg aaagaaacgc gtagcctgga tcgagcacaa gcgtctatcc atacagtaga
     2221 ttcgctgtgg accgagtgag gcgatgggga acgggaaaac agggcagaag atcgttgtcg
     2281 ggttaggcga gttgctttgg gatgtactgc ccgaaggcgc acgcctgggg ggagcgcccg
     2341 ccaattttgc ggtgatggcg ggccggctgg gcaatcgcgc ggtggtcgcc agccgggtgg
     2401 gcaccgatgc cctgggcaag caggcgcggg aggaactggc ggagctgccg gcggagagcg
     2461 attttctgca gtcggattct gaatatgcaa ccgggacggt gacgattcgg ctggagcacg
     2521 gcgagccgga gtttacgatc cacgaaccgg tggcctggga ccggctggcg atgacgccgg
     2581 aatggcgtga tctggcgcag caggcggatg cggtttgctt tggaaccctg gcgcaacggg
     2641 cgccggtgtc ccaggcgacg atcctggcgt ttctggacga gacacgacgg gattgcgtgc
     2701 gagtcttcga tgtgaacctg cggccgccgc actggtcggc cgagtcgctg cgcggaggaa
     2761 tggcgcgagc gacgatcctg aagctgaatg ccggcgagct gcggcatgtg ctgatgggaa
     2821 ccggagtgtg cccccatccg gtagagaagg ccgatgaaga cgaggtgctg cggggagcgc
     2881 ggcgcctgct ggaccgctat ccggtggaga tggtgtgtat cacgctggga gcgaagggga
     2941 gtttgctcgt gacgcggcat gagcatcacc ggcatcctgg tatgacggca accgtgaagg
     3001 acacagtagg cgcgggcgac gcgtttacgg cagcgctgac gcactattat ctggagggcg
     3061 cgccgttgcc gctgctgaat gaagcaggga accgatgggg cgcctgggtg gcctcgcaat
     3121 ccggcggcat gccgccgctc agggcggaga cgctggagcg agtgagcgcg gagattggag
     3181 cgcggcgggc gtaaggtagt cactgatggc gcacatccct tcttccaaag agcggcgggc
     3241 gctcggccag gcgcggcgca ggcaggtgcg acgacaggat cacgcacgct gggatgcgaa
     3301 gctgcgccag cacgatcctg tggctctgat cgccgagtcg atgcgcgggc gcgtgccggg
     3361 cctggtgggc ctgaagtacg agcggatgct ggcgtcgccc ttcgggttct ttcgcggcgc
     3421 tgttccggtg atggcggccg atcttgcgct gctgccgcac accggcatcg tgaatcagat
     3481 ttgcggtgac gcgcacgtgc gcaacctggg cgcatttgcc gctcccgacg ggcggctcct
     3541 gttcgacatc aacga
//
LOCUS       DASZYN010000365         2348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_529161, whole genome shotgun sequence.
ACCESSION   DASZYN010000365 DASZYN010000000
VERSION     DASZYN010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529161"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VHE33_09365"
     CDS             <1..199
                     /locus_tag="VHE33_09365"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HVT97704.1"
                     /translation="DANEALATFSLLRRWNVLLIEAALPQNAAKPVKHPERGEMTFST
                     IVETMAGHDLNHLGQLERMAE"
     gene            complement(272..>2348)
                     /locus_tag="VHE33_09370"
     CDS             complement(272..>2348)
                     /locus_tag="VHE33_09370"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HVT97705.1"
                     /translation="YETGLTIEPDLQSAIHLYKDVCNRGNQFACTQLGRVYTNGPESN
                     RNYAGAGELFSKACDAGEMAGCNNLGVLSYYGHGTTLDYKKANALFGKACDGNYIKGC
                     DSLAYGYLYGNGVAKDPEKARLLYKKACDGGEMGACTSLATMYENAQGLPKDYGQAAS
                     YYRRACSGGDMTGCTYLGLLYAAGNGVAKDESAASAFYRKACDGGEMAGCIDLGYEFE
                     FGQAVDKDPAEAVNLYRKACIAQEWSGCTHLANLYQTGSGIAKDEAQALRLFNRACDA
                     NDANACTNLGYFYNTGTMVAHDDARAVALYRKGCDGGEMTGCRDLGIMYFHGIGTAKD
                     PAQAFQLFDKNCSAGDAPSCTNLAFMYWSGQGTAADQPRSATLYRKACDGGDPAGCRA
                     LGTVYELGTGVPKDAVQAAVFYRKACDGSDGLSCAQLGLIYQQGLGVQPDPARAVALY
                     RKSCDAGNQDGCRGLGMALMQGTGVAKDETQAAGVFRKSCDAGDANSCRNVGIQYHNA
                     MGVPKDDALAAKAFRSGCDGKDSLSCSNLGLLYALGSGVDKDIAKAVDLFRSACDQNG
                     PEGCQLLGSFYLQGNGVDKDPAKAAALFGKACDAGLPTACGGLGYMLAQGTGVEKDPV
                     KAAPLFRKACDGGIGVACAALGVLMEQGTGVTKDAAQALVLYRKACDGGMKEACDKVK
                     TMAAAPATP"
BASE COUNT          409 a          745 c          736 g          458 t
ORIGIN      
        1 cgacgccaat gaagcgctgg cgaccttttc gctgctgcgg cggtggaatg tgctgctgat
       61 cgaggcagcg ctgccgcaga acgcagcgaa gccggtgaag catccggagc ggggcgagat
      121 gacgttttcg accattgtgg aaacgatggc ggggcacgat ctgaaccatc tggggcagct
      181 ggagcggatg gcggaatagg aatgggcctg gactcgtaag cccgaaaccc gggcgtcgcg
      241 gaactgcacg cagcgatggg ccgttgcccg ctcagggcgt ggctggagcc gccgccatgg
      301 ttttgacctt gtcgcaggcc tctttcatgc cgccgtcgca ggccttgcga taaaggacca
      361 gcgcctgcgc cgcgtctttc gtgacgccgg ttccctgctc catcaatacg ccgagcgctg
      421 cgcaggcaac gccaatgcct ccgtcacagg ccttgcgaaa gagcggggcc gctttgacgg
      481 ggtctttctc cacccccgtc ccctgcgcga gcatatagcc cagcccgccg caggcggtgg
      541 gaaggcccgc gtcacaggcc ttgccaaaca aagcagccgc cttggcggga tccttatcga
      601 ccccgttgcc ctgcaggtag aacgaaccga gaagctgaca accttcggga ccgttctgat
      661 cgcaggcgga gcgaaacagg tcgacggctt tggcgatgtc cttgtccact ccgctcccga
      721 gcgcatacaa aaggccgaga ttcgaacagg agagggaatc cttgccatcg cagccggagc
      781 gaaacgcttt cgccgccagc gcgtcgtcct tcgggacgcc catggcgttg tggtactgaa
      841 tgccgacatt gcggcagctg ttggcatcgc ccgcgtcgca ggatttgcgg aagacgccgg
      901 cggcctgggt ctcgtctttg gccacgccgg ttccctgcat gagggccatc cccagaccac
      961 ggcacccatc ctggtttccg gcgtcgcagc ttttgcgata cagcgccacc gcccgagccg
     1021 gatccggctg gacgccgagg ccctgctgat aaatgaggcc cagctgggcg cagcttaagc
     1081 cgtcggaacc gtcgcatgcc ttacggtaga agaccgccgc ctgcacggcg tcttttggga
     1141 cacctgtccc aagttcgtag accgtcccga gggcgcggca tccagccggg tcgcctccat
     1201 cgcacgcctt ccggtagagg gtggccgagc gcggctgatc ggccgccgtg ccctggccgc
     1261 tccagtacat gaacgccagg ttggtgcagc tcggagcatc gcccgcgctg cagttcttgt
     1321 cgaacagctg gaacgcctgc gccggatctt tcgcggttcc tatgccgtga aaatacatga
     1381 tgcccagatc gcggcatccg gtcatctctc cgccgtcgca acccttccga tagagcgcca
     1441 cagcccgtgc gtcgtcatga gcgaccatgg ttccggtgtt gtagaagtag ccgagattgg
     1501 tgcaggcgtt cgcgtcattc gcatcgcagg cccgattgaa caggcgcaaa gcctgcgctt
     1561 catccttcgc gatgccggag ccggtttggt aaaggttggc caggtgcgtg cagcccgacc
     1621 actcctgcgc gatgcaggcc ttgcggtaca aattcaccgc ttcggccgga tccttgtcca
     1681 cagcctgtcc aaactcaaac tcgtagccca gatcgatgca accggccatc tctccgccgt
     1741 cgcaagcctt acgataaaag gcggaggctg cgctctcgtc tttggcgacg ccattgccgg
     1801 ccgcatacag aagtcccaga taggtgcagc cggtcatgtc gccgccggag caggctcggc
     1861 ggtagtacga agccgcctgg ccgtagtctt tgggaagtcc ctgcgcattc tcatacatgg
     1921 tggcgagact ggtacaggcc cccatctcac cgccgtcaca cgccttcttg tagaggagcc
     1981 gcgccttttc cgggtctttg gcgacgccat tgccgtagag atagccatac gccaggctgt
     2041 cgcagccctt gatgtaattg ccgtcgcatg ctttgccgaa caatgcgttg gcttttttgt
     2101 agtcgagagt ggttccgtgc ccgtagtaag agagaacacc gagattgttg cagccggcca
     2161 tctctccggc gtcgcaggct ttgctgaaca gctcgcccgc gccggcatag ttgcggttcg
     2221 attccgggcc gttggtgtac acgcggccca gttgcgtgca ggcaaactgg tttccgcggt
     2281 tgcagacatc tttgtagagg tggatggcgc tctgcaggtc gggttcgatg gtgagcccgg
     2341 tttcatag
//
LOCUS       DASZYN010000366         8459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_206758, whole genome shotgun sequence.
ACCESSION   DASZYN010000366 DASZYN010000000
VERSION     DASZYN010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8459
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206758"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="VHE33_09375"
     CDS             complement(<1..1316)
                     /locus_tag="VHE33_09375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVT97706.1"
                     /translation="MRAVRLSACLLLFPAMAVFSAFGQQSVPDAPAPQASSQSQSAPP
                     NAPTPQTQPATPLPNLRNQVAPGKGTTPDEQGAAAAGQPAPQIGNGQVGQQTPQAAPP
                     PDSVQQTAPETFHPGQEPYTITTGVTEVIVPVTVKDKHGKEIPGLPWWRFRVFEDGIR
                     QRIAFFSTDAYPLSVAFVIDATLPSDVMAKVNRSLSVVSNGLTPADTVSVISYAGTGP
                     QMLTDFTAAQGPRLVAALDMAKKPGDQMGVPMTDGPFASGPTVNGLQVDPTLAPQRGN
                     ASGVLNLPKEIHPLNDAILYAAEQLASQPRGRRRVIYVISDGKNSRSKASYKEVVQYL
                     LAHNISVYGTNVGDAALWGIGYLDRAKLPFLQPEDVLPRYAIATGGDVDHQLSENGIQ
                     SAFVRITDSLRTAYTLIYNSHQPSISGKYHVIDVRVEGLTGLQVNA"
     gene            1570..2640
                     /locus_tag="VHE33_09380"
     CDS             1570..2640
                     /locus_tag="VHE33_09380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVT97707.1"
                     /translation="MRWRVFVLPILLASSAVPLTWGQAAPSPDAPPVSNFPADNSPTE
                     VNPKETLKVTSNLVSEYFTVRDKKNALIPFLTKNDCSVFEDKQQQKLTSFVAESNQPL
                     TLGILIDTSGSQQYVLPLEQQSGSAFLKRILRPKDEAFLLSFDVGVNLEQDYTSDARQ
                     LERAMNKVQINTAAGGGSVGVPGIGQGPVPTIGSPKGTLLYDAVYLASHNKMRSETGR
                     KALILLTDGEDQGSETKPKQAIEAAQNANAIIYVILIADRGFYFNSGWGMGYSGDAWM
                     KQMTEATGGHVINVGNNGKKLEDAFDQIEQELRTQYVAQYVPANKSMDGRFRKIDIAC
                     HDSDGDLKVQARKGYFAVGDEE"
     gene            complement(2740..3327)
                     /locus_tag="VHE33_09385"
     CDS             complement(2740..3327)
                     /locus_tag="VHE33_09385"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT97708.1"
                     /translation="MSPARSPCYVVCVTPTLETPRLILRPLALADAAQIQQIFPRWEI
                     VQYLNAVVPWPYPPDGAVSYIRQRALPAMARGDEWHWTLRRKEEPGRILGAIGLIRGE
                     RDHRGFWLAPEWHGQGLMTEAVIAVNDYWFDVLGFPVLRAPKAVANVASRRISEKTGM
                     RLIGTEERNLVSGRQLSEVWEITAEEWRGFRKRKQ"
     gene            complement(3338..3871)
                     /locus_tag="VHE33_09390"
     CDS             complement(3338..3871)
                     /locus_tag="VHE33_09390"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HVT97709.1"
                     /translation="MRIRLIPAASLVAVLASVTLALAQDAPVTPPPIKMGLWQTETTT
                     TMSGAPDNPMGRAMGGGGRSTVTQACFTPDTWTKEIRNAQQRRRAAECTQSNLHQDGH
                     KLTYDEVCSSQGGYSTNIHFEMLFDDPENGHGSMDMKTSGAAFPQGMTMHMTMKTKYL
                     SSDCGSVKPGEGKVVHP"
     gene            complement(4016..4498)
                     /locus_tag="VHE33_09395"
     CDS             complement(4016..4498)
                     /locus_tag="VHE33_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97710.1"
                     /translation="MLKKPRQRTWDGVLSELRRLQFDVQPVSGVANQQRISKGGCAIV
                     VEKADNAAGLRMIVRPGLVMNGEIAHLVDRGFQKFLKTSKTEFPATAERLKALHAFVE
                     ELKEVTGSVTLYNEALGTVSDAYLYDRVKGRDLPESQRPTPAWKRPENQGPRENARER
                     "
     gene            4633..5571
                     /locus_tag="VHE33_09400"
     CDS             4633..5571
                     /locus_tag="VHE33_09400"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HVT97711.1"
                     /translation="MATLTTTRTTTPGSTLLDRIGNTPLVRLERIAAELPGVQILAKA
                     EWANPGGSVKDRAAASIVLEAMRQEKLCPGMRLLDATSGNTGIAYAMLGAALAFPVTL
                     CMPSNVSPERKRILNAYGAEVVWTEPAESSDGAIRKAWELAKDPQYYYADQYSNEANW
                     RAHYGGTGREILDQTGGQITHFVAGLGTSGTFMGVARRLKEANPAIECVSMQPDSPFH
                     GLEGLKHMATAIVPKIYDPRLADRNVEIETEAAYAMAKRLARTEGLLVGISAAAAVVA
                     AVRVGEEQAAAGKEAVIVTILPDAADKYLSERFWEE"
     gene            5573..6082
                     /locus_tag="VHE33_09405"
     CDS             5573..6082
                     /locus_tag="VHE33_09405"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HVT97712.1"
                     /translation="MLRLSQQVYDAIRQHGEETYPHECCGVFLGRFVIEDGGAVNDVE
                     DAIRAGNTRTDSAHNRYNIAPQELVKIQRIGRERGLEIVGFYHSHPDHPAQWSPTDFA
                     EAHWLGCSYVITAVEKGAARQTNSFLLAGSTEDDKAFQNQQIEVTGQLDPGFEPEPEA
                     DSAPGFVAQ"
     gene            6243..6518
                     /locus_tag="VHE33_09410"
     CDS             6243..6518
                     /locus_tag="VHE33_09410"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HVT97713.1"
                     /translation="MTIEIPTPLRVYAGNQSSVNVDGTTVSDAVSELISRYPDFRKHL
                     YNPDGKLRSFVNLYLNDEDVRYLPEREGSTVKSSDTLTIIPSIAGGC"
     gene            6672..7847
                     /gene="moeB"
                     /locus_tag="VHE33_09415"
     CDS             6672..7847
                     /gene="moeB"
                     /locus_tag="VHE33_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257790190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HVT97714.1"
                     /translation="MATITEPVTLPQLSTEEMARYSRHLILPEVGMEGQQKLKAAKVL
                     CVGTGGLGSPLAFYLAAAGIGTLGLVDFDVVDSSNLQRQIIHSTKDVGRPKIQSATEK
                     LTALNPFLNVVQHETMLTSQNALEIVSQYDIIADGTDNFPTRYLVNDACVLTGKPNSY
                     ASIFRFEGQASVFATEEGPCYRCLYPEPPPPGLVPSCAEGGVLGILPGLLGVIQATEV
                     IKLILGKGEPLIGRLLLVNALDMKFRELKLRKNPECPVCGKNPTVTQLIDYQQFCGIV
                     PEAKVAAEKNGIPQMSVTELKQKRDAGEDVFVLDVREPYEYQIAQIGGHLIPLGELPN
                     RLGELDPAREIVVQCKSGGRSQRAAEFLAQNGFKKLHNLAGGITAWANEIDPDVAKY"
     gene            complement(7899..>8459)
                     /locus_tag="VHE33_09420"
     CDS             complement(7899..>8459)
                     /locus_tag="VHE33_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97715.1"
                     /translation="KDTTNGTIYGIPAAIPLAFFLGAFPTKTLFMIARRIGTQQLKLG
                     DDQETGNELEKLQGVSKSNAERFRDEGVTTIAELAWSDPIDLTIRTNFDFNYVVDCVS
                     QALAWIYLGSYCVNLAPCSLRGAQEIASLIEDAADSTDQAAQDRANKTIDAAAALLKI
                     DKDAFRSTLLQIAGDPYTEFLVNVWG"
BASE COUNT         1643 a         2492 c         2749 g         1575 t
ORIGIN      
        1 ttggcgttga cctggaggcc tgtcagccct tccacacgca cgtcgatcac gtggtatttg
       61 ccgctgatcg atggctggtg actgttgtag atcagcgtgt aggcggtgcg caatgaatcg
      121 gtgatccgga caaaggcgct ctggatgccg ttctcgctca gctgatgatc cacatcgccg
      181 ccggtggcaa tcgcgtagcg cggcagcacg tcttcgggct gcaggaaggg cagcttggcg
      241 cgatcgaggt agccgatgcc ccacagggcg gcgtcgccaa cattggttcc gtagaccgag
      301 atattgtggg cgagcagata ctggacgact tccttgtagc tggccttgct gcgcgagttc
      361 ttgccatcgc tgatgacgta gatcacgcga cgacggccgc ggggctggct ggccaactgt
      421 tcggctgcgt agaggatcgc gtcgttgagg ggatgaatct ccttgggcag gttcagcacg
      481 ccgctggcat tgccgcgctg gggcgccagg gtgggatcca cctgcagacc gttcaccgtg
      541 ggtccggaag cgaacggccc gtcggtcatg ggcacgccca tctgatcgcc gggcttcttc
      601 gccatgtcga gagctgcaac gagacgcggg ccctgggcgg cggtgaaatc ggtcagcatc
      661 tgcggaccgg tgccggcgta gctgatgacg gagacggtat ccgccggtgt caatccatta
      721 ctgaccaccg aaaggctgcg attgaccttg gccatgacgt ccgacggaag cgtggcatcg
      781 atgacgaacg caaccgagag aggataggcg tcggtggaga agaaggcgat ccgctgcctg
      841 attccatctt caaaaacacg gaaccgccac catggcaaac cagggatttc cttaccgtgt
      901 ttgtccttca cggtgaccgg gacgatgacc tcggtgacgc cggtggtgat ggtgtagggt
      961 tcctgaccgg ggtggaaggt ttcgggcgcg gtctgctgga ctgaatccgg tggaggagcc
     1021 gcctgcgggg tctgctggcc cacctggccg ttgccgatct gcggtgcggg ctgcccggcg
     1081 gctgcagcgc cctgctcgtc gggggtagtg cctttgccgg gggcgacctg attgcgaagg
     1141 ttgggtagcg gagtggccgg ctgggtctgc ggagtcgggg cgttgggcgg ggcggactgg
     1201 ctctgggaag acgcctgcgg agccggcgca tccgggacac tctgctgacc gaaggcggaa
     1261 aacacagcca tcgccggaaa aagcaacaaa caagcgctga gccggactgc ccgcatcacg
     1321 ctgtaacccc ctgaaacttt caatactgcc ccttccgaaa ccgttctcag ccgcgtccct
     1381 tttcctcgat tttgcccgag tctccgaaga tggtgcgcgg tgcttccatt ggctttagat
     1441 gcacgctgga aagacaggac ggcgtatgct caagattaac agagcgacag gcgcgcactg
     1501 ttccgaataa ccccggcgaa gaataaccca acctggtaac gctccgtcta accgcaagga
     1561 gaactggccg tgcgctggcg tgtatttgtc ttgcccatcc tgcttgcgag tagtgctgtc
     1621 cctctgacgt gggggcaggc cgctccgtca cccgacgcgc cgccggtgag caactttccc
     1681 gccgacaatt cgccgaccga agtcaatccc aaagaaacgc tgaaggtgac ttcgaacctg
     1741 gtcagcgagt atttcacggt ccgcgacaag aagaacgcgc tcatcccctt tctgacgaag
     1801 aatgactgtt cggtgtttga ggacaaacag cagcagaagc tcaccagctt cgtggccgag
     1861 tcgaaccagc ccctcacgct ggggatcctg atcgatacca gcggcagcca gcagtatgtt
     1921 ctgcccctcg agcagcagtc gggttcagct tttctcaagc gcatcctgcg gccgaaggac
     1981 gaggcgtttc tgctgagctt cgacgtcggt gtgaacctcg aacaggatta caccagcgac
     2041 gcgcgtcaac tggagcgcgc catgaacaag gtgcagatca acacggcggc ggggggcggc
     2101 tccgtaggcg tgccgggcat tgggcagggg ccggtgccga ccatcggcag tcccaaaggg
     2161 acgctgctgt acgacgcggt gtacctggct tcgcacaaca agatgcgctc ggaaacgggc
     2221 cgcaaggcgc tgatcctgct cacggacggg gaggaccagg gctcggagac gaagccgaag
     2281 caggccatcg aagccgcgca gaacgccaac gcgatcattt acgtgatcct gatcgccgac
     2341 cggggcttct acttcaacag cggatggggg atgggctact ccggcgacgc gtggatgaag
     2401 caaatgaccg aggcgacggg cggccacgtg attaacgtcg gcaataacgg caagaagctc
     2461 gaagatgctt tcgatcagat cgagcaggaa ctgcggacgc agtacgtcgc gcagtatgtg
     2521 ccggcaaaca agtcgatgga tgggcggttc cggaagatcg atattgcctg ccacgattcg
     2581 gacggcgatc tgaaggtcca ggcgcgcaag ggatatttcg cggttggcga tgaagagtga
     2641 gaagagcagc gtttagcggc aagcgtatgg cgttcgagac agtgttgttg agagagagtc
     2701 tctggcgctg ccgtttacac cctacatcct atcctccgct cactgcttcc tctttcggaa
     2761 ccctcgccac tcctccgcgg tgatctccca aacttcgctc agctgccggc cggacacaag
     2821 gttgcgctcc tcagtcccaa tcaatcgcat gccggtcttt tccgagatgc ggcgggaggc
     2881 cacattcgcc accgccttgg gagcacgcag gaccgggaag ccgaggacat cgaaccagta
     2941 gtcattgacc gcgatgaccg cctcggtcat caggccctgt ccatgccatt ccggcgccag
     3001 ccaaaatccg cggtggtccc gctcgccgcg gatcagtccg atggcgccca ggatccggcc
     3061 cggctcttcc ttccggcgca gggtccagtg ccactcatcg ccacgggcca tcgcgggtaa
     3121 ggcgcgctgg cgaatgtaac tcaccgcacc gtcgggcggg taaggccagg gaacgacggc
     3181 attgagatac tgcacgatct cccagcgggg aaagatctgc tggatttgcg cggcatctgc
     3241 gagcgcgagc gggcgaagga tcagccgcgg cgtttcgagg gtgggagtca cgcagacaac
     3301 atagcagggc gagcgcgccg ggctcacccg agcatcatca cgggtggacg actttgccct
     3361 ctcccggctt aacgctgccg cagtccgagc tgaggtattt cgtcttcatc gtcatgtgca
     3421 tggtcatgcc ctggggaaag gccgcgccgc tggtcttcat gtccatggag ccatgaccgt
     3481 tctcgggatc gtcgaaaagc atttcgaaat ggatattcgt ggaatagcca ccctggctcg
     3541 aacagacctc gtcgtaagta agcttgtggc cgtcctggtg caggttggat tgagtacact
     3601 cggcggcccg ccggcgctgc tgcgcattcc ggatctcctt cgtccacgta tcgggcgtga
     3661 agcaggcctg cgtgaccgtg gatcgaccgc ccccgcccat ggcccggccc atcgggttgt
     3721 ccggcgctcc ggacatggtc gtcgtggttt cggtttgcca gaggcccatt ttgatcggcg
     3781 gcggcgtgac aggcgcatct tgtgcgagcg ccaaagtaac ggaagcaagg accgcgacga
     3841 gcgatgcggc agggatcagg cgaattctca tggagggctc cctcgtgggt gatcttcagg
     3901 tgaggagcgt tatagcacgg ctcgcgctcg tgtgactatg cgtgccgatc gaggtgactc
     3961 gaaacgccgc ctcattcagc ccgggagtct cggcggcctc ctggacggcc agtcctcagc
     4021 gttcgcgtgc gttctcgcga ggtccctggt tttcgggccg tttccacgcg ggggtgggcc
     4081 gctggctctc cggcaaatcg cggcctttca cgcggtcata gagataggca tcgctcaccg
     4141 tccccagcgc ttcgttgtaa agggtcaccg agccggtgac ttccttcagc tcttcgacga
     4201 aggcatgaag agctttcagc cgctcggctg tggcgggaaa ttcggtctta ctggtcttca
     4261 ggaatttctg gaacccgcgg tccacgaggt gggcgatttc gccgttcatc accaggccgg
     4321 ggcggacgat catgcgcaga cccgcggcat tgtccgcttt ttcgacgaca atggcgcagc
     4381 cgcctttcga gatacgctgc tgatttgcga ccccgctgac gggctgaaca tcgaattgaa
     4441 gccggcggag ttcactgaga actccatccc acgtgcgctg gcgcggtttc ttaagcatga
     4501 tggcggtttt cccgatgaag gcaccaaccg ctacactgaa cctaagcggg aggctgtagt
     4561 ttcagggtcg cacacggggg cgacggctgc aatcgaccgc tgcatccatt agtaatgagc
     4621 tctggatcgg gaatggcaac actcaccaca accaggacga cgactccggg gtcgacgctg
     4681 ctcgatcgca tcgggaacac gccgctggta cgcctggagc gcatcgccgc ggagctgccc
     4741 ggcgtgcaga tcctggctaa ggcggagtgg gcgaacccgg gcggatcggt gaaggatcgc
     4801 gccgcggcct ccattgtgct cgaagctatg cgccaggaga agctgtgccc gggtatgcgg
     4861 cttctggatg cgaccagcgg gaacacaggc attgcgtacg ccatgctggg ggctgcgctg
     4921 gcctttcccg tgacgctttg catgccgtcg aacgtttctc ccgagcgcaa acggattttg
     4981 aacgcctacg gagccgaggt ggtctggacc gagcctgcgg agagcagcga cggcgccatc
     5041 cggaaggcgt gggaactggc gaaggatccg cagtattact acgccgatca atacagcaac
     5101 gaggccaact ggcgggcgca ctacggcggg acgggcagag aaatcctcga ccagaccggc
     5161 gggcagatca cgcacttcgt tgcgggcctg ggaacgagcg gcacgttcat gggcgtggcg
     5221 cggcggctga aggaagcgaa tccggcaatc gagtgcgtct ccatgcagcc ggactcgccg
     5281 tttcatggtc tggaggggct gaagcacatg gccacggcga ttgttccgaa gatctacgat
     5341 cccaggctcg ccgaccggaa cgtcgagatt gaaaccgaag cagcgtacgc gatggcgaag
     5401 cgtctggcgc gcaccgaggg cctgctggtg ggcatttcag ctgctgctgc agtcgtggcg
     5461 gcggtgcggg tcggcgaaga acaggccgcg gcgggaaaag aagccgtgat tgttacgatt
     5521 ttgcccgacg cggcggacaa atacctgagc gaacggttct gggaggaata gattgcttcg
     5581 gctgagccag caggtctacg acgcgatccg ccagcacggc gaggaaacgt atccccatga
     5641 gtgctgcggc gtgtttctcg gccgattcgt gatcgaggac ggcggggccg tgaatgacgt
     5701 tgaggatgcg atccgcgccg ggaatacccg aaccgactcg gcccacaatc gctacaacat
     5761 cgcgccgcag gagctggtga agatccagcg cattgggcgc gagagggggc ttgagattgt
     5821 cgggttctac cactcgcacc ccgatcatcc ggcgcagtgg tccccaaccg acttcgccga
     5881 ggcccactgg ctgggctgca gctacgtgat tacagccgtc gaaaagggcg cggcgcggca
     5941 gacgaattcg tttctgctgg ccggatcgac tgaggacgat aaggcctttc aaaaccagca
     6001 gatcgaggtg actggccagc tcgatcccgg attcgagccc gagccggagg cagacagcgc
     6061 gccgggtttt gttgcacaat agaaagtgaa gatggcgaac cttcccgaga ccggcttcga
     6121 gcgcatgcct tctgcggcgc tgtgttgctg tctctgtacc atggcacgac gcccgtcgtg
     6181 ccgctagcgc ctcttcgtct ccttttctga accgatcgca gtccctaacc caaggacaag
     6241 ctatgactat cgaaattcct acccctttgc gggtgtatgc gggcaatcag tccagcgtga
     6301 atgtggacgg aaccaccgtt tcggatgctg tctcggagct gatctcgcgc tatccggact
     6361 ttcgcaaaca cctttacaac ccggacggca aactgcgctc gttcgtcaac ctgtatctca
     6421 atgacgaaga cgtgcggtat ttgcccgagc gcgaagggag caccgtgaag agctccgaca
     6481 cgctcaccat catcccctcc atcgccggcg ggtgctagcc gcacggcgga cgcggcttcg
     6541 ccgcaaggct aaacctggcg ctacgctccc tggcagcacg tgcgtctcct gcaattggcg
     6601 cgaaccgcac agaccgggac gacctgtccc ttgcaccgcg catgacacaa gccgcatcct
     6661 taaggaacac catggccacg atcaccgaac ctgttaccct cccgcagctc tccaccgaag
     6721 aaatggcgcg ctattcgcgc cacctgatcc tgcccgaggt gggcatggag gggcagcaga
     6781 agctgaaggc ggcgaaggtg ctgtgcgtcg gcacgggcgg cctgggttcg cccctggcgt
     6841 tctacctggc ggcagcgggc attggaacgc tggggctggt ggactttgac gtcgtcgatt
     6901 ccagcaatct gcagcggcag atcattcaca gcacgaagga cgtgggccgg ccgaagatcc
     6961 aatccgcgac cgagaagctc accgcgctga atccgttcct gaacgtggtg cagcacgaga
     7021 cgatgctgac gagccagaac gcgctcgaga tcgtttcgca gtacgacatc atcgccgacg
     7081 gcaccgacaa tttccccacg cgctatctgg tcaacgatgc ctgcgtactg acgggcaagc
     7141 ccaattccta tgcatcgatc tttcgtttcg aagggcaggc cagcgtcttc gcgacggagg
     7201 aaggcccatg ctaccgctgc ctgtacccgg aaccgccacc gcccggcctg gtcccgtcgt
     7261 gcgctgaagg cggggtgctg gggatcctgc ccggactcct cggggtgatc caggcgacag
     7321 aagtgatcaa gctgatcctc ggcaagggcg agccgctgat cggccggctg ctgctggtga
     7381 atgcgctcga catgaagttc cgcgagctga agctcaggaa gaatccggaa tgcccggtgt
     7441 gcgggaagaa ccccaccgtg acacaattga tcgactatca gcagttctgt ggcattgttc
     7501 cggaggcaaa ggtggcagca gaaaagaacg gcatcccgca aatgagcgtg accgagttga
     7561 agcagaaacg cgatgccggc gaagatgttt tcgttctcga cgtgcgcgaa ccgtacgagt
     7621 atcagattgc gcagattggc gggcacctga tcccactggg cgagctgccc aatcgcctgg
     7681 gtgagctgga tccagcgcga gagatcgtgg tgcagtgcaa gtccggtgga cgcagccagc
     7741 gcgcggcaga gttcctggcg cagaacggct tcaaaaagct gcacaatctg gcgggcggga
     7801 ttacggcgtg ggccaacgag atcgatcccg acgtggcgaa gtactaaggc gcacctcgga
     7861 gcaattccgg agagatcggg cgcgacgcgg cgttcgcact agccccacac attgacgaga
     7921 aactctgtgt aggggtctcc ggcaatctgc agcagggtgg agcggaacgc gtccttatcg
     7981 attttgagca gagcggcggc agcgtcgatc gtcttgttgg cgcgatcctg ggcggcctga
     8041 tcggtgctgt cggcggcatc ttcgatgagc gaagcgattt cctgggctcc gcgcagggag
     8101 cagggcgcga gattgacaca gtagcttccc agatagatcc acgccaaagc ctggcttacg
     8161 cagtcgacca cgtaattgaa atcgaagttg gtgcgaatgg tgagatcgat tggatccgac
     8221 caggccagct ctgcaattgt ggtgacgcct tcatcgcgaa agcgctccgc attgcttttg
     8281 gatacgccct ggagtttctc cagctcattg ccggtttcct ggtcgtcgcc cagctttagc
     8341 tgctgagtgc cgatgcgacg ggcaatcatg aagagcgttt tggtggggaa ggcacccaga
     8401 aagaaagcga gcgggatggc cgcgggaatg ccgtagatcg tgccgttggt ggtgtcttt
//
LOCUS       DASZYN010000367         2729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_466439, whole genome shotgun sequence.
ACCESSION   DASZYN010000367 DASZYN010000000
VERSION     DASZYN010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466439"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VHE33_09425"
     CDS             complement(<1..231)
                     /locus_tag="VHE33_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97716.1"
                     /translation="MRVIRLVGAAVLIGACLPVAVAMGQDTTAAVTVVHCPSLFDSAA
                     GKMLGPTTVVIAGDKFEKVESGTQSEPGATVIE"
     gene            complement(318..2570)
                     /locus_tag="VHE33_09430"
     CDS             complement(318..2570)
                     /locus_tag="VHE33_09430"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin receptor-like dimerization
                     domain-containing protein"
                     /protein_id="HVT97717.1"
                     /translation="MRLAIGAVCLFAVTAYAVDAGHNLRGYTPEDSAKEVSWEQKFRE
                     LPDRDHIRENMRRLSARPHHVGSPYDKDNAEWLVAQLKSYGLNAQIEEFSALFPTPKS
                     RKLELLGATPFTAKLEEPAIGVDPTSAQTAEQLPTYNAYSRDGDVTAPLVYVNFGRPE
                     DYDELDRMGVSVKGAIVIARYGMSWRGIKPKVAAEHGAVGCIIYSDPHEDGYYRGDSY
                     PEGPSRPAEGAQRGSVMDMPVYPGDPQTPGVGAVAGVKLIPLDQVQTITKIPVLPISY
                     ADAIPFLKALGGEVVPESWRGALPFTYHVGPSTVKAHLALSFNWDRKPLRDVIMKIPG
                     KKYPDEWVIRGNHHDAWVNGAEDPISGASPELEEARAFGELLKQGWQPDRTIIYCWWD
                     GEEPALLGSTEWAETHADELKQHAVVYFNSDGNGRGYFRAEGSHSLENFVNDVAKDIQ
                     DPETGMNVWKRVRLVDMSRAKAQDRHEIRTRADLRIPALGSGSDYTVFIDHLGVASVN
                     FGYGGEDDGGGQYHSVYDDFYWYTHFSDTDFSYGRALAQTAGTMVMRMADADMLPFQF
                     GDLADTVHMYDTQLKTLLNEKRDEAKERDEEIADGVYKALYDPKKPMVAPPTEAIPPY
                     LNFAPLDQASDDLTDAAKAFDQAFGAANGTASEGLNADLIRSERLLTDQRGLPNRPWF
                     EHVLYAPGFYTGYGVKTIPGVREAIEQKKWSEADEQIGRVSAALEHEADLLKKATKLL
                     PEKKAEAAGF"
BASE COUNT          524 a          979 c          680 g          546 t
ORIGIN      
        1 ctcaatcacc gtcgctcccg gttcgctctg cgtcccggac tcgacctttt cgaacttatc
       61 gcccgcaatc accaccgtgg tcggtcccag catcttgccg gcggcactgt cgaacaaact
      121 ggggcaatga accaccgtca cagcggccgt cgtgtcctga cccatcgcca cagcaaccgg
      181 caaacaagcg ccaatcaaca cagcagcccc caccaaacga atcactctca tccgtctctc
      241 ctcatcggca ttccaccgcg cgcagtcagg cgttcctatt gcctattgcc tattgcctgt
      301 tgcctattgc ctacctttca gaatcccgcc gcctccgcct tcttctccgg cagcagcttc
      361 gtcgccttct tcagcaaatc cgcctcatgc tccagcgcgg cggaaacgcg cccgatctgc
      421 tcatccgcct cgctccattt cttctgctcg atcgcttcac gcacgcccgg aatcgtcttc
      481 acgccatagc ccgtgtaaaa gcccggcgcg tacagcacat gctcaaacca cggccgattc
      541 ggcaatcccc gctgatcggt cagcaggcgt tcgctgcgga tcaaatcggc attcagcccc
      601 tccgacgcag tgccattcgc cgcaccaaag gcctgatcga acgccttcgc cgcatcggtc
      661 aagtcgtccg atgcctgatc cagcggcgcg aagttcagat acggcggaat cgcctccgtc
      721 ggcggtgcca ccatcggctt cttcggatcg tagagcgcct tgtacacccc atcggcgatc
      781 tcctcgtcgc gctccttggc ctcgtcgcgt ttctcgttca gcagcgtctt caactgcgtg
      841 tcatacatgt gtaccgtatc cgccagatcc ccgaactgga acggcagcat gtccgcatcc
      901 gccatacgca tcaccatcgt gccggccgtt tgcgccagcg cccgaccata actgaagtcc
      961 gtgtcggaga aatgcgtgta ccagtagaag tcgtcataga cggagtggta ctgcccgccc
     1021 ccatcatcct cgccgccgta tccgaaattc acgctggcca cgccgaggtg atcgatgaac
     1081 acggtgtagt ccgagcccga gcccagcgct ggaatccgca gatccgcccg cgtgcgaatc
     1141 tcatggcggt cctgagcttt cgcgcgcgac atatccacca gccgaacgcg tttccacaca
     1201 ttcatgcccg tctcgggatc ctgaatgtct ttcgcaacgt cgttcacaaa gttttccagc
     1261 gaatgcgatc cctccgcccg aaagtagccg cgcccattgc catcggagtt gaaataaacc
     1321 accgcgtgct gcttcagctc atccgcgtgg gtctcggccc actccgtcga gcccagcagc
     1381 gccggctcct cgccatccca ccagcagtag atgatcgttc gatccggctg ccagccctgc
     1441 ttcagcaact cgccgaacgc gcgcgcctct tccagctcag gactcgctcc cgaaattgga
     1501 tcctccgcgc cattcaccca cgcatcgtgg tggttcccgc ggatcaccca ctcatcggga
     1561 tacttctttc caggaatctt catgattacg tcgcgcagcg gcttgcgatc ccagttgaag
     1621 ctcaacgcca ggtgcgcttt caccgtgctc ggcccaacat gataggtaaa cggcaaggcg
     1681 ccgcgccatg attcgggcac gacctctcca cccagagctt tcagaaacgg tatcgcatcg
     1741 gcatacgaaa tcggcagcac cggaatcttc gtaatggtct gcacctggtc cagcggaatc
     1801 agcttcacac ccgccactgc gcccactccc ggcgtctgcg gatcgcccgg atacaccggc
     1861 atgtccatca cgctgccgcg ctgcgcgccc tccgccggcc gggacggccc ctcgggatag
     1921 gaatcgccgc gatagtagcc gtcttcgtgc gggtcggaat aaatgatgca tcccaccgca
     1981 ccatgctcag cagccacctt cggcttgatg ccgcgccagc tcatgccgta gcgtgcaatc
     2041 acgatggcgc ccttcaccga aacacccatc cggtccagtt cgtcgtagtc ttcgggccgt
     2101 ccgaaattca catacaccag cggtgccgtc acgtccccgt cacgcgaata cgcgttgtac
     2161 gtcggcagtt gctcggcggt ctgtgccgac gtcggatcca caccgatcgc cggctcctcc
     2221 agcttcgccg tgaacggtgt cgcccccagc agctccagct tccgcgactt cggcgttggg
     2281 aacagcgccg aaaactcctc gatctgcgcg ttcaggccat acgatttcag ctgcgccaca
     2341 agccattcgg cattgtcctt gtcatagggc gaccccacgt gatgcggccg cgccgaaagc
     2401 cgccgcatgt tctcgcgaat gtgatcgcga tccggcagct cacgaaactt ctgctcccag
     2461 ctgacctctt ttgcggaatc ctcgggagtg tagccgcgca ggttgtgccc tgcatcaacg
     2521 gcatacgcag tcactgcaaa cagacagaca gcaccaatcg ccagacgcat acggtgttcc
     2581 tcacagggca aattgaataa gttactgggg aagtgacaga ttgcagattg cgtgaaccca
     2641 acactataga ccaaccggtt gcaccgcagc gatcccaggc ccgatataac gaattcttct
     2701 tccgccgatg caatttgcgc ccttggcga
//
LOCUS       DASZYN010000368         6385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1108876, whole genome shotgun sequence.
ACCESSION   DASZYN010000368 DASZYN010000000
VERSION     DASZYN010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108876"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /gene="dnaA"
                     /locus_tag="VHE33_09435"
     CDS             complement(<1..1208)
                     /gene="dnaA"
                     /locus_tag="VHE33_09435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HVT97718.1"
                     /translation="MSFAVTPSVALNPWVQILAALEKKVNRQSFDTWLKPTRYSYTRE
                     RMLYVRIPTPEFKHVGDRYGDLIQEAIENLHLEFDDVAFITPDEDPTIPRVREDGGFA
                     PSSSTAIPNLHNGGGARPAQPVGPQQARFDWSTAAQLNARYTFDAFVCGPGNQFARAA
                     AMAVAESPAKAYNPLFLYGGVGMGKTHLMHAIGHEVKLRQPGASISYVSAEKFTNEMI
                     SSVRFDKMTSFRDRFRTVDLLLIDDIQFLSQKERTQEEFFHTFNALHENMKQIVIASD
                     RPPRELAEVEDRLRSRFEWGLIADIQPPDLETKVAILQKKAENEHLPQPLPMDVALFI
                     ASNVRTNVRELEGALVRLIAWCGLNGLEITLPTAQQCLKQFIDTQVRKITIESIQRAV
                     AEQFGMRVAEL"
     gene            1704..1859
                     /gene="rpmH"
                     /locus_tag="VHE33_09440"
     CDS             1704..1859
                     /gene="rpmH"
                     /locus_tag="VHE33_09440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HVT97719.1"
                     /translation="MPKRTFQPNRRSRSKTHGFRSRMKTKSGAAVLSRRRAKGRKRVS
                     VSAGYRD"
     gene            1899..2318
                     /gene="rnpA"
                     /locus_tag="VHE33_09445"
     CDS             1899..2318
                     /gene="rnpA"
                     /locus_tag="VHE33_09445"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HVT97720.1"
                     /translation="MAATPPGLRQNEDMPEARLDWRSARLRKHADYQRVYRDSRRHTS
                     ASMSYFFRARSSEEGAGAQPRVGLTAGRVLGGAVERNRIRRRMREAVRLHLSELPPRV
                     DVVLHPRKSVLEIEFARLESEVARVFASVTAGAEAKK"
     gene            2315..2641
                     /gene="yidD"
                     /locus_tag="VHE33_09450"
     CDS             2315..2641
                     /gene="yidD"
                     /locus_tag="VHE33_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HVT97721.1"
                     /translation="MKTSPMQQLRRVRSTAARAWLALYKQAVSPSLHAIAPGACRFQP
                     TCSEYAAIAVAEHGMLRGGLMALGRLLRCHPLSRGGFDPVPAKDSASHIHPTSSAISC
                     VPQTDR"
     gene            2650..4470
                     /gene="yidC"
                     /locus_tag="VHE33_09455"
     CDS             2650..4470
                     /gene="yidC"
                     /locus_tag="VHE33_09455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HVT97722.1"
                     /translation="MAEIQNPNQQSGGASGGGGGGDMRSLFGFMVVFLIMVIAFQYFS
                     PKKPEQVAQQQKSQPAQTQPSAPAPVPASAGSTPQPASGHGHAAPADAAAVQSSAESD
                     TVVENELYRITFTNRGGAVKSWLLKKYKDDTQKAPLDLVNHAAAAKFGLPLSLFTYDA
                     SLKNRLNSSLYVPSQTGTVSVAGSGSKTLTFDYSYGGLVVRKTFHFDSSYVIGADFSV
                     TQNGAPVTALLAWPSGLGDQTTLQGYAGATFASSQNGKTDETAAKKVVGGDTLRGSFD
                     YAGVSDLYFAAVFMPTSPADTAVVTQHETLDVPKNPEHPDPKAVDQMPLLGAAVGSVG
                     GGAFSTRLFVGPKLLDLLDSVHTTTGENLEHVVSFGWWGWIAKPMLLVLRFFVTHGIP
                     NWGWAILILTLILNVAMLPTRVMMMKSSLKMQRIQPQMDAIKAKYAKYKTTDPRRQEM
                     NKEMFDLQRKEGVNMFGGCLPMLLQYPLLFGFYRMLEYAIELRQAHWLWLTDLSAPDA
                     MHILPLSVIVSMFLSQFFTPSPGMDQKQQRMMAFMMPAVFGVMMWNIGSGVALYWAGS
                     NLLGVATQMVMNRTSMGVEMRAIAAKRAAKRGAKTINARR"
     gene            4551..5102
                     /locus_tag="VHE33_09460"
     CDS             4551..5102
                     /locus_tag="VHE33_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HVT97723.1"
                     /translation="MPIDDYSAAAQKIAEFLNSLIHLGRLRLKFRITAGPGAADPAGL
                     EAREIYVELAGPDTGLLTQRGGELLRALEHVAAKVLRLENEEHDKVSFDAENFKALRA
                     RELKLAAETAAERVRRTGQPYSFAPMSSRERRMLHLAFRSYTDLETASSGEGLRRFVV
                     AYPKGYERQDASPRESRFSGRRR"
     gene            5199..>6385
                     /gene="mnmE"
                     /locus_tag="VHE33_09465"
     CDS             5199..>6385
                     /gene="mnmE"
                     /locus_tag="VHE33_09465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HVT97724.1"
                     /translation="MQPHPQTRERESIAQETIVAISTPPGRGGIGIVRLSGTHALPIA
                     QGLVRSSTTFEHARARVADVVDPEHEQKIDEAVVTYFARPNSYTGEDLVEIAAHGSPV
                     VLELLVRMALRAGARLARPGEFTERAFLAGRIDLTQAEAVRDLIEAQTLHQARIAAEQ
                     MGGALSRRIQPAKQNLVNLIALLEAGIDFAEDDVEVTPDLEIVTRTDTISGELQSIAR
                     SFDYGRLVHSGLRLAIVGRPNVGKSSLFNCLLERERAIVTAQPGTTRDLVSDRLSVGG
                     IPVELVDTAGLREAKNEAEAIGIRKSREMLADADIVMVVLDASVPLRDDERELIASLE
                     GRRALIVRNKSDLNDSSELDSELPLPCVATSALSGAGIPELREALAESVRNPGAEGES
                     GVLT"
BASE COUNT         1253 a         1917 c         2021 g         1194 t
ORIGIN      
        1 agctcggcca cgcgcatgcc gaattgctcg gcgacggcgc gctggatcga ttcgatggtg
       61 atcttgcgca cctgggtatc gatgaactgc ttgaggcact gctgcgcggt gggcagcgtg
      121 atctcgaggc cattcaggcc gcaccatgcg atgagccgca ccagcgcgcc ttccagctcg
      181 cgcacgttgg tccgcacatt tgaggcaatg aacaacgcca catccatggg cagcggctgc
      241 ggcaggtgct cgttctccgc tttcttctgc aggatggcga ccttggtttc cagatcgggc
      301 ggctggatgt ccgcgatgag cccccactcg aagcggctgc gcaggcggtc ctccacctcg
      361 gccagctcgc gcggcggccg atcggaagcg atgacgatct gcttcatgtt ctcgtgcaga
      421 gcgttgaagg tgtggaagaa ctcttcctgg gtgcgttcct tctgcgagag gaactgaatg
      481 tcgtcgatga gcagcagatc cacggtgcgg aagcggtcgc ggaagctggt catcttgtcg
      541 aagcgcaccg agctgatcat ctcgttggtg aacttctcgg ccgagacata cgaaatggac
      601 gcgcccggct ggcgcagctt cacttcgtga ccgatggcgt gcatcagatg cgtcttgccc
      661 atgcccacgc cgccgtagag gaagagcggg ttgtaggctt tcgcggggct ttcggcgacg
      721 gccatggcgg cggcgcgcgc gaactgattg ccggggccgc agacgaaggc gtcgaacgtg
      781 tagcgggcgt tcagctgcgc ggccgtcgac cagtcgaaac gagcctgttg cgggccaacg
      841 ggctgtgccg gccgggcgcc gccgccgttg tgcaggttcg ggatcgccgt cgaggaggat
      901 ggtgcgaagc cgccgtcttc gcgcacgcgc ggaatggtgg gatcctcatc gggagtaatg
      961 aaggcgacgt cgtcaaattc gagatgcaga ttctcgatcg cttcctgaat gagatcgccg
     1021 tagcgatcgc cgacatgttt gaactcggga gtcgggatgc gcacgtacaa catgcgctcg
     1081 cgcgtgtagc tgtagcgcgt cggtttcagc caggtatcga acgattgccg gttcaccttc
     1141 ttttcgagcg cagccaggat ttgaacccag ggattgagag caacagaagg tgtaacggca
     1201 aatgacatgc gcaatccttt tgtgacaaac gcccgggagc agaagtgacc gctggcgccg
     1261 caggccgaaa tgcggaaatg cccaaggtga tccctggagt gtggaaacag ggcgaatgca
     1321 ggggcaattc tttactggcc gggagtggct ccgatcagtc acgcatcgga gcgacggtca
     1381 aatttcttcc gacaaccgca gggcgtcgaa aaaatcttaa gtgaacgttg ccgatagtag
     1441 cacagccgaa ggcgaagaga agcgacatcg gaacagaaaa atttctcacg cacgaaagtt
     1501 gcacagatgc tggtgaaaaa agtaagagag cgcgattttt gcgggcaaat atgcgatcag
     1561 atatactcaa tacttgccgc gatgaggcgt gttctgtgtc attgcgcggc caatccgagc
     1621 tgttgcgcgt ccgcgaaaaa tgaacgaagc gacgcgatgc gcatccaggc gaaatcgaag
     1681 aagttgagga gagaagtttt tccatgccca agcgtacgtt tcaacccaat cgccgcagcc
     1741 gttcgaagac gcacggcttc cgcagccgca tgaagacgaa gagcggcgcc gcggtcctct
     1801 cgcgccggcg cgccaaaggc cgcaagcgtg tctcggtcag cgcagggtat cgcgactaga
     1861 ttcacggatt tcatcctctc aacccggccg cggatcgcat ggccgccact cccccaggat
     1921 tgcgacagaa tgaagatatg ccggaggcaa ggttggactg gcgcagcgcc cgtctgcgga
     1981 aacacgctga ctaccaacgc gtgtatcgcg acagccgccg gcacacctcc gcgtccatga
     2041 gctacttctt ccgtgcacga tcgagcgagg aaggggcggg cgcacagccc cgggtgggcc
     2101 tgactgcggg acgagttctg ggcggcgcag tggaacggaa tcgcatccgc cggcgtatgc
     2161 gtgaagcagt gcgcctgcac ctgagcgaat tgccgccgcg cgtcgatgtg gtgctgcatc
     2221 cgcgcaaaag cgttctcgag atcgagtttg cgcgtctgga gagcgaagtg gcgcgtgtct
     2281 ttgccagtgt gacggccggt gcggaggcaa aaaagtgaag acgagcccga tgcagcagct
     2341 tcgcagggtg cgatcgaccg cggcacgcgc atggctggca ctttacaagc aagccgtttc
     2401 gccatccctt catgccattg cacccggcgc ctgccgcttt cagccaacgt gttctgaata
     2461 tgcagcgatc gcggtcgccg agcatggcat gctgcgcggc ggcctgatgg cgctgggacg
     2521 tttgctgcgg tgccaccccc tgagccgagg tggctttgat ccggttcccg cgaaggacag
     2581 cgcttcgcac attcacccaa ccagcagcgc aatttcctgc gttccgcaga cagacaggta
     2641 acggaagtct tggcagagat acaaaatccc aatcagcaga gcggcggcgc ttccggtggc
     2701 ggcggcggtg gcgacatgcg ttcgctcttc ggcttcatgg tcgtttttct gatcatggtg
     2761 attgcctttc agtacttctc gccgaagaag ccggagcagg tggcccagca gcagaagagc
     2821 cagcccgcgc agacccagcc aagtgcgcca gcgccggtac cagcgagcgc cggctcgact
     2881 ccgcaaccgg cttccggcca tggtcatgcg gcgcccgcgg atgctgctgc ggtgcagtct
     2941 tcggctgaat ccgacacggt cgtcgagaac gagctttacc gcatcacctt caccaatcgc
     3001 ggcggagcgg tgaagtcctg gctcctgaag aagtacaaag acgacacgca gaaggccccg
     3061 ctggatctgg tgaatcacgc ggcggccgcg aagtttggtt tgccgctgtc gctgttcacc
     3121 tatgacgcca gcctgaagaa ccggctcaac tcctctctct atgttccgtc gcaaaccgga
     3181 accgtcagcg tggcgggaag cggttcgaag acactcacgt tcgactactc ctatggcggg
     3241 ctcgttgtcc gcaagacctt ccacttcgac tccagctacg tgatcggcgc cgacttttcc
     3301 gtcacgcaga atggcgcgcc ggtcaccgcg ctgctggcct ggccctccgg actcggtgat
     3361 cagaccacgc tgcagggcta cgccggggca acgttcgctt cctcgcaaaa cggcaagacc
     3421 gatgaaactg ccgccaagaa ggttgtgggc ggcgataccc tgcgcggctc gttcgattat
     3481 gcaggcgtca gcgacctgta tttcgcggcg gtctttatgc cgacgtcgcc ggccgacacg
     3541 gccgtggtca cacagcatga aacgctggac gtgccgaaga atccggagca ccccgatccg
     3601 aaggccgtcg atcagatgcc gctgctggga gcggccgtgg gctcggttgg tggcggcgcg
     3661 ttcagcacgc ggcttttcgt cggccccaag cttctggatc tgctcgactc ggttcatacc
     3721 accaccggag aaaacctcga gcacgtggtg agcttcggct ggtggggctg gatcgccaag
     3781 cccatgctgc tggtgctgcg cttcttcgtc acgcacggca tccccaactg gggctgggcg
     3841 atcctgattc tcacgctcat cctcaacgtc gcgatgctgc cgacgcgcgt gatgatgatg
     3901 aagtcttcgc tcaagatgca gcgcatccag ccgcagatgg atgccatcaa ggcgaagtac
     3961 gcgaaatata aaaccacgga cccgcgccgc caggaaatga acaaggagat gttcgacctg
     4021 cagcggaaag agggcgtgaa tatgttcggc ggctgcctgc cgatgctgct gcagtatccg
     4081 ctgctgttcg gtttctaccg gatgctggag tacgccatcg agctgcgcca ggcgcactgg
     4141 ctctggctga ccgatctctc cgcgcccgac gcgatgcaca tcctgccgct gtccgtgatc
     4201 gtctcgatgt tcctgtcgca gttttttacg ccttcgccag gcatggacca gaagcagcag
     4261 cgcatgatgg ccttcatgat gccggcggtt ttcggtgtca tgatgtggaa catcggctcc
     4321 ggtgtggcgc tgtactgggc gggcagcaac ctgctcggtg tggcgaccca gatggtgatg
     4381 aaccgcacca gcatgggcgt tgagatgcgt gcgattgccg cgaagcgtgc cgcgaaacgc
     4441 ggagccaaaa ccatcaacgc ccggcgttaa attgcgacca cggcggaatc agtggctaca
     4501 gcatcgtcaa cgtgatctaa taactccggg accggttcac taatagctca atgccgattg
     4561 acgattactc cgccgccgca caaaaaatcg cagaattcct gaacagcctg atccatctgg
     4621 gccgtctgcg cctgaagttt cgtatcactg cggggccggg cgctgcggat cccgccggcc
     4681 tggaggcgcg cgaaatctat gtggagttag ccggcccgga taccgggctg ctcacccagc
     4741 gtggaggcga actgctgcgc gccctcgagc acgtcgcagc caaagtgctg cggcttgaga
     4801 atgaggaaca cgacaaggtt tccttcgacg ctgaaaactt caaggctctg cgcgcgcgcg
     4861 aactgaagct ggcagccgag actgccgcgg agcgcgttcg ccgcaccgga cagccctaca
     4921 gctttgcgcc catgagttcc cgcgagcgac ggatgctgca cctggctttt cgctcctata
     4981 ccgatcttga aacggcttcg tccggcgaag gcctgaggcg cttcgttgtc gcgtacccga
     5041 aggggtatga gcgccaagac gccagcccgc gagagagccg cttttccgga cgccgccgct
     5101 aacgaaaagc caggacagaa agcagatcca ctcaactcgc ggctaatttg ccggtcgcgc
     5161 caaatgcggg cgcgaccaat tcagaaggct cgctcaggat gcagcctcat ccacaaacaa
     5221 gagaacggga gtcgatcgcg caggagacga tcgttgctat ctccactccg cccgggcgtg
     5281 gaggaatcgg catcgtgcgg ctctccggga cgcatgcgct tccgattgcc cagggcctcg
     5341 tccgcagttc cacgacattc gagcatgcgc gcgcacgcgt tgccgatgtt gtcgatcccg
     5401 agcatgaaca gaaaattgac gaagctgttg ttacctattt cgcgcgcccg aattcttata
     5461 cgggcgagga tctggtcgag atcgccgcgc acggttcacc cgtagttctg gaactgctgg
     5521 tgcggatggc gctgcgggca ggagcaaggc tggcccggcc cggcgagttc acggaacgcg
     5581 cgtttctggc gggccgcatc gatctgaccc aggcagaagc ggtgcgcgac ctgatcgaag
     5641 cgcagaccct ccaccaggca cgcatcgccg ccgagcagat gggtggggca ctgtcgcgtc
     5701 gcatccagcc tgcgaaacaa aacctggtca atctgattgc gcttctcgaa gccggcatcg
     5761 attttgccga agacgacgtc gaagtcacgc ccgatctgga aattgtcaca cgcacggata
     5821 cgatttccgg cgagctccag tcgatcgcgc gctcctttga ttatgggcgc ctggtgcaca
     5881 gcggattgcg tttggccatc gtgggccgtc ccaatgtcgg caaatcgtcg ctgtttaatt
     5941 gcctgctcga gcgggagcgc gccatcgtga cagcgcagcc tggaaccacc cgcgacctcg
     6001 tgagcgatcg tctttcagtc ggcgggattc ctgtggaact tgtggatact gccggcctgc
     6061 gagaagcgaa gaacgaagcc gaggcgatcg ggatccgcaa atcccgcgag atgctggccg
     6121 atgcggacat cgtgatggtc gtgctcgatg cttcggttcc actgcgcgac gatgagcgcg
     6181 agctgatcgc gtcgctcgag ggacgccgcg ctctcatcgt ccggaataaa tccgacctga
     6241 acgacagctc cgagttggat tcggaacttc cgttgccctg cgtagcgact tcagcgctga
     6301 gcggagcggg aattccggag ctgcgcgaag cgctggccga atcggtccgc aatcccggag
     6361 cggaaggcga atccggcgtc cttac
//
LOCUS       DASZYN010000369         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_978320, whole genome shotgun sequence.
ACCESSION   DASZYN010000369 DASZYN010000000
VERSION     DASZYN010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978320"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..916
                     /locus_tag="VHE33_09470"
     CDS             248..916
                     /locus_tag="VHE33_09470"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HVT97725.1"
                     /translation="MQRLLEGYTRFRRDVFPQHRDRFRLLADRQQPDVLFITCADSRI
                     VPDLILQTEPGDMFLCRAVGNIVPPHGSMPGGISSTIEYAVEVLHVRHIIVCGHSDCG
                     AIKAVLPRDNTDTRDHSKLPQLPLTSKWLGYIEPAWKYLPPGTSRDDQRALHTALIHA
                     NVIAQLENLKTHPEVARAVAHSELQIHGWYYDILTGNVEAFDQQERRFLALEDWAAAN
                     PKKS"
     gene            1057..1635
                     /locus_tag="VHE33_09475"
     CDS             1057..1635
                     /locus_tag="VHE33_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HVT97726.1"
                     /translation="MAQRVLGQEKVMRQNLDSSLALLARTPASLDALLRGLPDAWTLR
                     NEGDGTWTVRDVVAHLADLERTDWMPRVQRLLDCGETRAFDPVDRQAFRRNARGKSMA
                     RLLGEFARRRQKNLRDLRTLKLTAPDFGRRGQHPSLGRVTLAELLAAWTVHDLTHLHQ
                     ITRILAHQYRQATGPWERYLGVLQCNGHSASA"
     gene            complement(1767..>2141)
                     /locus_tag="VHE33_09480"
     CDS             complement(1767..>2141)
                     /locus_tag="VHE33_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97727.1"
                     /translation="IPSVLGLALGARYYWRRRHVWHWRIDGAALLLVSTMVSPYSWPF
                     DQLLMLPAIMHVCSVRTGRRWTPWLAVVNAAAVLLLLMKVPLSSPVYAWSATACLIWY
                     VWTRWRAGLGGVKEEKRELAAV"
BASE COUNT          429 a          787 c          558 g          367 t
ORIGIN      
        1 gtcctcatcg gcatcgtcgt ctccgcaatt ccccccggag aagcaaccag caaactcggc
       61 ttcgagatgc agttgctgat cggcaccgtg atctcaatcc tcatcgggtt ggcgctctac
      121 tggcgcggcg cacgcgcgaa gaacagagtt tagagtttgg agtttagagt cggcttcaaa
      181 ctctaatccc taaactctaa gctctcgctc ctgtacgctc tccgctgaac gctatacgct
      241 aacaaagatg caacgtctcc tcgagggcta cacccgattc cgccgcgacg tcttcccgca
      301 gcaccgtgac cgcttccgcc tgctcgccga ccgccagcag cccgatgtgc tcttcatcac
      361 ctgcgccgat tcgcgcatcg tccccgacct gatcctccag accgagccgg gcgacatgtt
      421 cctctgccgc gctgtcggca acatcgttcc cccgcacggc tccatgcccg gcggcatctc
      481 ttccaccatc gaatacgcgg tcgaggtcct gcacgtccgc cacatcatcg tctgcggcca
      541 ttccgactgc ggcgccatca aggcggtcct gcccagggac aacaccgaca cccgcgacca
      601 cagcaagctg cctcagctgc ccctcacgtc aaaatggctt ggctacatcg agcccgcctg
      661 gaaatatctg ccccccggca cttctcgcga cgatcaacgc gccctccaca cggcgctcat
      721 ccacgccaac gttatcgcgc agctcgaaaa cctgaagaca catcccgaag tcgcacgcgc
      781 cgtcgcccac agcgaattgc aaatccacgg ctggtactac gacatcctca ccggaaacgt
      841 cgaagcgttc gaccaacagg aacgccgctt cctcgcgctc gaagactggg cagccgccaa
      901 cccaaaaaag tcctgaaccc cagccgttga gcactgaatc cattgaactg gtatggcgtt
      961 tcggagaccc ttcaggttgt catcccgacc gaagcgcagc ggagcttgag ggacctgctt
     1021 tgttccccgc atcatggcta aaagaatttt agaaagatgg cacaacgagt tctcggacag
     1081 gagaaggtga tgcggcagaa cctggattcc agcctcgccc tgctcgcccg taccccggcc
     1141 tcactcgacg cgctgctgcg cggcctgcct gacgcttgga ccctgcgcaa tgaaggcgac
     1201 ggcacctgga ccgtgcgcga cgtcgtcgcc cacctggcgg acctcgaacg caccgactgg
     1261 atgccgcgcg tccaacgcct cctcgattgc ggcgaaacgc gtgccttcga tcccgtcgat
     1321 cgccaggcct tccgccgtaa cgcacgtggc aaatcgatgg cgcgactgct cggcgagttc
     1381 gcgcgtcgtc gccagaagaa tctcagagat ctgcgcacgc tgaaactcac tgcgcccgat
     1441 ttcggccgcc gcggtcagca cccatcactg ggtcgcgtga ccctcgcgga attgctcgcc
     1501 gcatggaccg tgcacgatct gacccacctg caccagatca cccgcatcct ggcgcatcag
     1561 tatcgccaag cgacaggccc gtgggaacgg tatctgggcg tgctccagtg caacggacac
     1621 agcgcctcgg cctgaatcgc gcctcgcagg tggaaacctt ccgggctttt gtaacagggc
     1681 acgacctcgg tcgtgccgac acggctcaac gtgaatcggg ctttagcccc tgcggctttt
     1741 cggcgcagcg tctttgcttt ccggccctaa accgccgcca gctcgcgttt ttcttccttc
     1801 accccgccaa gccccgctcg ccatcgcgtc cacacatacc aaatcaaaca cgcggtcgcg
     1861 ctccacgcat acaccggcga actcagcggc accttcatca gcagcaacag cacagccgcc
     1921 gcattgacga ccgccagcca cggggtccat cgccggccgg tccgcacgga acacacgtgc
     1981 atgatcgccg gaagcatcaa cagctgatcg aacggccacg aatagggcga caccatcgtt
     2041 gacaccagca gcaacgccgc gccgtcgatg cgccagtgcc acacatggcg ccgccgccag
     2101 tagtagcgcg cgcccagcgc cagccccagc accgaaggaa t
//
LOCUS       DASZYN010000370         1994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_466679, whole genome shotgun sequence.
ACCESSION   DASZYN010000370 DASZYN010000000
VERSION     DASZYN010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466679"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1267
                     /locus_tag="VHE33_09485"
     CDS             92..1267
                     /locus_tag="VHE33_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HVT97728.1"
                     /translation="MLVIADRPATRATIRTDIGAIFVSLELSKSIWLITALSPGGGEK
                     MSKHSVSGGDICGLLAHFARLREKARARTGKHFTVIVIHEAGLDGFWIDRVLCKENGI
                     ESHIVDPASIAVPRRSRRAKTDGIDGETLVRALLAYKRGEPRVCTMLRVPTPEEEDRR
                     RISRERKVLTAERVRHVNRIKGLLFSQGVTGYEPLRRDRRKRLEELETGDGRTLPAHL
                     KGQLCRELDRLELLLEQIKAVEVERDTLLAAERTDAVAPGPLAMLLDIKGIGPEFAAV
                     LGTEGLFRHFDNRRQVAAYAGLAPTPWRSGSIDREQGISKAGNPRLRATLIQLAWLWL
                     RHQPGSALSRWFQERVKRNGGRLRKTMIVALARKLLVALWKYVTAGVIIEGAVRKAV"
BASE COUNT          363 a          621 c          648 g          362 t
ORIGIN      
        1 tcagatccta tgacgattgg cggatagcga cacgcgctgc ttttgctgtg gcaaagtgtg
       61 gttgctgaca ccactctcat tggaggcaat catgctcgtc atcgctgacc gacccgctac
      121 gcgtgccacc atccgaacgg atattggcgc aattttcgtg tcgttggaac tgagtaagtc
      181 gatctggctg atcacggcgc tgtcgccggg tggcggcgag aagatgtcga agcattcggt
      241 ttcgggcggt gacatttgcg gactgcttgc gcactttgcg cggcttcgcg agaaggcacg
      301 ggcgcgcacc ggcaagcatt ttacggtcat cgtcatccac gaggcagggc tcgacggctt
      361 ctggatcgat cgcgtgcttt gcaaggagaa tgggattgaa agccacatcg tcgatccggc
      421 atcgatcgcg gtcccgcgcc ggtctcgccg tgccaagacc gacgggatcg atggcgagac
      481 gctggtgcgt gcgctgcttg cctacaaacg aggcgaaccg cgcgtctgca ccatgctgcg
      541 ggtcccgacg ccggaggaag aggatcgccg ccgcatctcg cgtgagcgga aagtgctgac
      601 cgccgagcgc gtacgacacg tcaaccgcat caaggggctg ctgttcagcc agggcgtcac
      661 tggctacgaa ccgctgcgcc gtgatcgacg caagcggctg gaggaacttg agaccggcga
      721 tggtcgcaca ctgcccgctc acctgaaagg ccagttgtgc cgcgaactcg accggctcga
      781 actgctgctc gagcagatca aggcggtgga ggtcgaacgc gacacgctgc ttgccgcgga
      841 gcgaactgac gctgtcgcgc cgggcccgct ggctatgctg ctcgacatca aggggatcgg
      901 tcccgagttc gccgccgtgc ttgggaccga agggctgttt cggcacttcg acaaccggcg
      961 acaggtagcg gcctatgcgg gactggcgcc gacaccctgg cggagtggat cgatcgatcg
     1021 tgaacagggc atctcgaaag ccggcaatcc ccggctgcgc gccacgctga tccagctcgc
     1081 ctggctatgg ttgcgacacc agccgggctc ggctctgtcg cgctggttcc aggagcgggt
     1141 gaagcgcaat ggcggccgtc tgagaaagac gatgatcgtt gcgctcgcac gaaagctgct
     1201 ggtggcgctg tggaaatacg tgaccgctgg cgtcatcatc gaaggtgcgg tgaggaaagc
     1261 tgtttgagca gtcgccccag catattccaa tccccaggac ccgatcagtc ctgcggatcc
     1321 aggtggacga accgtcgtta accatggccc aagcgccgga actcagaatg gtctcgtctt
     1381 cctgagcccc tgcccgccgc acgcgggatt atggtgcagc cgcccgcagc ggcgaccgaa
     1441 tgtgaggttg atcgtgctcg gagacgagcc gcgtcatgca attggggcca gacctgaatc
     1501 ctggaagcca aaaggaacct gccaatgttg tcctgaaaca cgcggcgtcc tcccccttga
     1561 ccccgaactc gtcatgtggg gttaacagac gattatcccc cctccgcctc gcattcgctc
     1621 ggcgcctccc cccgcttcgc gggtggcgga tgggcgccga gccggcggcc agggcctatc
     1681 ctctccctca cgaagtgaag gagaggtgtc cgcgaagcgg acggagaggg ggtagaaaag
     1741 caacaattaa cgcgagttca gagccgtgcc cgttagggcg cggcgtgctc gcccagcgaa
     1801 gaatctctca ttacctatcg aagggcttgt cccgtcaggc tcacgccctc acacgtcgcc
     1861 tatcgcgccg gggtgcgccc tcggcgccgc tttcattggc gggcttgttg cgcaacggac
     1921 cgatccgcgc gaccacgtcg gccaccgtcg gccgcccggc tttcgtgtgc gaatcgagcg
     1981 cttcgcgcat cgct
//
LOCUS       DASZYN010000371         2925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_783695, whole genome shotgun sequence.
ACCESSION   DASZYN010000371 DASZYN010000000
VERSION     DASZYN010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783695"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..>2925
                     /locus_tag="VHE33_09490"
     CDS             259..>2925
                     /locus_tag="VHE33_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ
                     domain-containing protein"
                     /protein_id="HVT97729.1"
                     /translation="MKSSITKFTLNLALSYALLSVSLVAASAQTAATRPPLIPQPREF
                     ASRPDVSLSGGVRILVPGGNSDDRFAAKTLNEALKARGVRVSESASALRIWLLRDDTA
                     AARRVLHREKMPFDAAMQPEGYVLVASPHEAFVIAHTSAGVFYGAQTLIQLERRGGAG
                     PVLQGAAIRDWPAMRWRGVHDDLSRGPVPTLAYQEQQIRTFAAYKINVYSPYFENTMQ
                     YKSNPLPALPGGSLSAADAKALVEYAKPYHITVIPEQEAFGHLHKVLLWQQYAPLAEV
                     PNGAVLAPGQPGTIKLITQWFDELAGIYPGPFLHIGADETFQLGQGQTAEAVKQRGLG
                     AVYVDLLSRIHEALTPLHRQLLFWGDIAMNSPDEIPHLPHDMIAVAWHYEPEPQGFAR
                     WLDPYTHAGMQTWVAPGVNNWNRVWPNFEMALGNIQGFVADGQKAGSTGMLNTVWDDD
                     GEGLFAEDWYAILFGAAAGWQPGTSDIARFQQDYGPVFHGDQSDAVNQAQLALIAAHQ
                     ALVRAGLEDARDAYFWADPWSPEGQKIDAKIRPVAAEVRLDAERALTLLAQARASGNL
                     RNPEALDAMDLGARRVDFLAFKFQVADQIADSYLRLYNGQQNHDIAMHTSRDLWDLSG
                     VNGLCEDLRDGYDFLHDRYSEVWLAENRPFWLNNVTVRYDDAARLWVERGEKISAARA
                     QWQQTHTLPSPQEIGIAPTSPAEAGVTQAQPAAPADHPGHVATGIDVLEKDRFAELGA
                     LAQAHQGHLRLGLLTNQTGVDSAGRRTIDVLKHDAATAVPGLTLTALFSPEHGISGSY
                     DQPGVPNSTDTASGLPVISLYGSKEQDRRPSLESLRKLDAVAIDLQDAGVRYYTYEAV
                     VRYFLEAAAQTGTTIVVLDRPDPIGGGLVQGPV"
BASE COUNT          539 a          990 c          826 g          570 t
ORIGIN      
        1 aactacagcg gcgtgaccgt ttccaacacg agcagtctct ttggatacgt caccagctat
       61 cgcgatccgc gcatcatcca gcttggcggg aaactgaact tctaggcccg tcttcgtgat
      121 caatcggccg ggattccgac agggtcccgg ccacttttct tgatgcctgc gcaggttcgc
      181 ttcacaattc tggccagttc tgcgcgatcc cttcccttct gccttacgga cccctgtttc
      241 atgtgtgaga atcgacccgt gaaatcctcc attacaaaat tcactctgaa tcttgccctg
      301 agctatgccc tcctctccgt ctctcttgtc gcagccagcg cgcaaaccgc cgcgactcgg
      361 cctccgctga tcccacaacc ccgcgagttc gcctcccgtc ccgacgtttc cctttccggc
      421 ggagttcgca ttctcgtacc tggggggaat tccgacgatc gcttcgcagc gaagaccctg
      481 aatgaagcgc tgaaggctcg gggtgtccgc gtatccgaat cagcctccgc cctccggatt
      541 tggcttctgc gcgacgacac cgccgcggcc cggcgagtac tgcatcgcga gaagatgcct
      601 ttcgacgccg caatgcagcc cgaaggctat gtactcgtcg ccagcccgca cgaggctttc
      661 gtgattgccc acacctctgc cggcgtcttc tacggcgcgc agaccctcat ccagcttgag
      721 cgccgcggtg gcgcgggtcc ggtccttcag ggtgctgcga ttcgcgactg gcccgccatg
      781 cgctggcgtg gcgttcatga cgatctgtcc cgcggcccgg tgccgacact tgcgtaccag
      841 gaacagcaga tccgcacttt tgccgcgtac aaaatcaacg tgtactcgcc ctacttcgag
      901 aacaccatgc agtacaagtc gaatccgctg cccgctctgc ctggtggctc gctttcggca
      961 gcggacgcga aggcactggt tgaatatgcg aagccctacc acatcaccgt cattcccgag
     1021 caggaggcct tcggccatct gcacaaggtc ctgctttggc agcagtacgc accgctcgca
     1081 gaagttccca acggcgccgt gctcgctccc ggtcaacccg gaaccatcaa gctgatcaca
     1141 caatggttcg atgagctggc gggaatctat cccggtccgt tcctccacat cggcgctgac
     1201 gagacgttcc agctgggcca gggtcaaacc gccgaagcag tgaagcagcg cggcctcggc
     1261 gccgtctacg tcgaccttct gtctcggatt catgaagccc tcacgcctct gcaccggcag
     1321 ctcctgttct ggggtgacat tgccatgaac agtcctgacg agattccgca tctgccccac
     1381 gacatgatcg ctgtcgcctg gcactatgag cccgagcctc agggcttcgc acgctggctc
     1441 gatccttaca cgcacgctgg aatgcaaacc tgggttgcac cgggcgtgaa caactggaat
     1501 cgcgtctggc cgaatttcga aatggctctg ggcaatatcc agggtttcgt cgccgacggc
     1561 caaaaggccg gcagcaccgg catgctcaat accgtctggg atgatgacgg cgagggcctc
     1621 ttcgcggagg attggtatgc catcctcttc ggcgccgcag caggatggca gcccggcacc
     1681 agcgacatcg cccgcttcca gcaggattac ggccccgtct ttcacggcga ccagagcgat
     1741 gccgtgaacc aggctcagct tgcacttatc gcggcgcacc aggcactggt ccgcgcagga
     1801 ctcgaggatg cacgtgacgc gtacttctgg gccgatccgt ggagcccgga aggacagaaa
     1861 atcgacgcga agattcgtcc cgtggccgcc gaagtccgcc tcgatgcgga acgggctctc
     1921 acgctgcttg cgcaggcacg ggcatctggc aatttgcgca atcctgaagc cctggatgcg
     1981 atggatctgg gtgcgcgacg cgtcgacttc cttgccttca agttccaggt cgccgaccag
     2041 atcgccgact cctacctgcg tctctacaac ggccagcaga accacgacat cgccatgcac
     2101 acctcccgcg atctgtggga tctctcggga gtgaacggcc tctgcgagga tttacgcgac
     2161 ggttacgact tccttcatga ccggtacagc gaagtctggc tcgcggagaa tcgtcccttc
     2221 tggctcaaca atgtcaccgt ccgctacgac gatgcggcgc gcctctgggt ggagcgcggt
     2281 gaaaagatca gcgctgcccg cgcccaatgg cagcagacgc atacgctgcc ctcgccccag
     2341 gaaatcggca tcgccccaac ctcaccggct gaggcgggcg tgactcaggc gcaacctgcg
     2401 gcgcccgccg atcacccagg tcacgtcgca accggaatcg atgtgctgga gaaggatcgt
     2461 tttgccgaac tgggtgccct tgcccaggcg caccaggggc acctgcgcct gggcctgctc
     2521 acgaatcaaa ccggggttga ctcggcaggc cgccgcacaa ttgatgtcct gaagcacgat
     2581 gccgccactg cggttcctgg cctgacgctg accgccctgt ttagccccga acacggcatc
     2641 agtggatctt acgaccagcc cggcgttccc aactctaccg acacggcgtc cggcctaccc
     2701 gtcatcagcc tctatggttc aaaagaacag gaccggcgcc catctctcga atcgctgcgc
     2761 aaactcgatg ccgtcgccat cgatctgcag gatgcgggcg ttcggtatta cacctacgaa
     2821 gcggttgtga ggtattttct cgaagctgcg gcgcaaactg ggacgaccat cgtcgtgctc
     2881 gatcggcccg atcccatcgg cggtgggctc gtacagggtc cggta
//
LOCUS       DASZYN010000372         1957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_77805, whole genome shotgun sequence.
ACCESSION   DASZYN010000372 DASZYN010000000
VERSION     DASZYN010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77805"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1129
                     /locus_tag="VHE33_09495"
     CDS             17..1129
                     /locus_tag="VHE33_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HVT97730.1"
                     /translation="MRWTVRLMVAGLAMFAVATSQGAVASQEGGAAHGTKPVALFYMT
                     NDPKSIRDFFAHSSQIDLLVPTWYQVDENGLVTGEPEPAVLAQAKSEKLPVMPIITSF
                     NKKSFHLLATSARAQEEMNAALVRECRLHGYSGIQFDFENIEWTDRDALSALVAKSAA
                     VLHGAGFQLTIATVPNAPGYPGAGGFAKWIYTDWRGAYDLAAISKAVDLVCLMTYDQN
                     TRWTTPGPVAGWQWTVDNMQYALKVVPKEKLSLGIPLYGYHWYTGAPTVTPPTHEGEL
                     PVEKPNPTADYIGVPDALLLAHDWNGKIQWDDEDHTAWFYFYRDQMREWIFFTDLRTF
                     RERYELAQQNGLEGFCSWVLGEEDPAIWTFLPKRRP"
     gene            1153..>1957
                     /locus_tag="VHE33_09500"
     CDS             1153..>1957
                     /locus_tag="VHE33_09500"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HVT97731.1"
                     /translation="MAIPSGESMQYFLGVDAGGTKTTFVLGDASREVARVQTGTIKRM
                     KADAETAEANLRRGLEELRARTGVERGNVARCCVGTAGETVPLVVDWLRDAFARHVGG
                     ELIIEGDVDVALDSVFFGKRGVLVLAGTGSNVAGRAATGEIVTAGGWGPALADQGSGH
                     FLGLEGLRRGFLAIDERRTTRLLAIAMEFWKLGSLGELIQFANANPAPEYSKLAPLVV
                     EAAEAGDAVAKEVVAQGGSDLAMLAGLVIEHLRDLEASSDKEFVVPAVAV"
BASE COUNT          364 a          462 c          751 g          380 t
ORIGIN      
        1 ttgaggtgct tgcggtatga gatggacggt tcgtctgatg gtggctgggt tggcgatgtt
       61 cgcggtggcg acgagccagg gtgcggttgc gagtcaggaa ggcggggccg cgcatggaac
      121 gaaaccggtt gcgctgttct atatgacgaa cgacccgaag tcgattcgcg attttttcgc
      181 gcactcgtcg cagatcgatt tgctggtgcc gacgtggtat caggtggacg agaacggtct
      241 ggtgaccggg gagccggagc cagccgttct ggcccaggcg aagagcgaaa agctgccggt
      301 gatgccgatc atcacatcgt ttaacaagaa gagcttccac ttgctggcaa cgagtgcgcg
      361 cgcgcaggag gagatgaacg cggcgctggt ccgcgagtgc aggctgcacg gttattcagg
      421 gattcagttt gattttgaaa atatcgagtg gacagaccgt gatgcgctga gcgcgctggt
      481 ggcgaagagt gcggcagtgt tgcacggcgc cggtttccag ctgacgattg cgaccgtacc
      541 gaacgcgccg ggatatccgg gcgcgggcgg atttgcgaag tggatctata cggactggcg
      601 gggagcctac gatctggcgg cgatcagcaa ggccgtggat ctggtgtgcc tgatgacgta
      661 cgaccagaac acgcgctgga cgactccggg accggtggcg ggttggcagt ggaccgtcga
      721 caacatgcaa tatgcgctga aggtggtgcc gaaggagaag ctgtcgctgg ggattccgct
      781 ttacggatat cactggtata cgggtgcgcc gacggtgact cccccgaccc acgagggaga
      841 actgccggtg gagaaaccca atccgacggc ggattacatc ggcgtgccgg atgcgctgtt
      901 gctggcgcac gactggaacg ggaagattca gtgggacgat gaggatcaca cggcctggtt
      961 ctacttctac cgtgaccaga tgcgagaatg gattttcttt accgatctgc ggacgtttcg
     1021 cgaacggtat gaactcgccc agcaaaacgg gctggaaggt ttctgctcgt gggttctggg
     1081 cgaagaagat ccggcgatct ggacgtttct gccgaaacga cgtccctagc aaacttcgcg
     1141 tcgcggggcg cgatggcgat tccgagtgga gagagcatgc agtatttcct cggcgtggat
     1201 gccggcggca cgaagaccac ttttgttttg ggcgacgcga gccgcgaagt ggctcgagtg
     1261 cagacgggca ccatcaaacg gatgaaagcg gatgcagaga cggccgaagc caacctgcgg
     1321 cgagggctgg aagagctccg cgccaggacg ggagtcgagc gggggaatgt tgcacgttgc
     1381 tgtgtgggga cggcggggga gacggtgccc ctggtggtgg actggctgcg cgatgcgttt
     1441 gcgcggcatg ttggaggaga gctgatcatc gagggcgatg tggatgttgc cctggactcg
     1501 gtgttctttg ggaagagggg tgtgctggtt ctggcgggga cggggtcgaa cgtcgcgggg
     1561 cgtgcggcga ctggcgagat cgtgaccgcg ggaggatggg gaccggcgct ggcggatcag
     1621 gggtcaggac attttctggg actggaaggc ctgcggcggg gatttttggc gatcgatgag
     1681 cggcggacga cgcggctcct tgctattgcg atggaattct ggaagctggg ctcgcttggg
     1741 gaactgatcc agttcgcgaa cgcgaatccg gcaccggaat attcgaagct ggcaccgctg
     1801 gtggttgagg ctgcagaggc gggcgatgcg gtggcgaagg aagtggtggc gcagggcggg
     1861 tcggatctgg cgatgctggc gggtctggtg attgagcatc tgcgcgacct cgaggcgagc
     1921 agcgacaagg agtttgtggt tccggcggtg gccgttg
//
LOCUS       DASZYN010000373         1685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1041169, whole genome shotgun sequence.
ACCESSION   DASZYN010000373 DASZYN010000000
VERSION     DASZYN010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041169"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1685)
                     /locus_tag="VHE33_09505"
     CDS             complement(<1..>1685)
                     /locus_tag="VHE33_09505"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 protein"
                     /protein_id="HVT97732.1"
                     /translation="IEIRSIHVIKSVHGAGVQLNGPDADDRVLSDSFSEDTPQLKIMD
                     LGDAEDRIHRGFGSQLIYNRNSGLSLFLGALSADRLLTVFHLQSSAKPNPRVLSFDAE
                     DTGTNEALREEQWATRYGSANVVPLRLHVAPGEVLSSERLAFSIGSDYHAQLEDYGKA
                     IRVLRHARVQAPTLMGWWSWTAYYYGVSEGTTLTNAEWLAQNLKPLGYRYFQVDEGYQ
                     FARGEYATADGKAYPHGMAFVGGRVRGLGLTFGLWVAPFEVSERSWVYEHHKDWLVHN
                     LQGDPIHIGRIGTFDELYVLDTTNPGAQAYLTQTYTTLVNEWGVRFLKLDFMDAAAVE
                     GFFYRPNTTALEAQRIGLEVIRRAVGDNVLLDKDGSPMLTPVGIVDAGRISQDTGHTF
                     GSTRDAATGVAARYYMNHNFFESDPDAFTVSEQIVPDRGWHGNKTPLSLNEAESSIAL
                     SAVSGGMFEIGDDLPELGKSPERLALVRNRDLLDMARLGRASVPVDLMSYRPEDLEPS
                     IFLLREDDRQTILTIFNWSEKPTTHDLTLAELGLRATAYDATDILRSIAVPIG"
BASE COUNT          324 a          494 c          533 g          334 t
ORIGIN      
        1 ctgccgatcg gtacagcgat gcttcgcaga atgtctgtgg cgtcatacgc agtggccctg
       61 aggccgagtt cggcgagagt gaggtcgtgg gttgtgggct tctcgctcca gttgaagatg
      121 gtcaggatcg tctgcctgtc gtcttccctg agcaggaaga tgctgggctc gaggtcttca
      181 ggccggtagc tcatcaaatc gaccggcacc gaagcgcggc cgagacgcgc catatccagc
      241 agatcgcgat tgcgtacgag cgcaagccgt tcgggcgact tgcccaactc cggcaggtcg
      301 tcgccgattt cgaacatgcc tccggaaacc gcggacagcg cgatcgagct ttctgcttca
      361 ttcagagaca gaggggtctt gtttccgtgc cacccgcgat cgggcacgat ctgctcggag
      421 acggtgaagg catccggatc cgactcaaag aaattgtggt tcatgtagta gcgagctgcc
      481 actccggttg cagcgtcgcg ggtcgagccg aaggtgtggc cggtgtcctg cgatatgcgt
      541 cccgcatcca caatgccgac aggcgtcagc atcgggctgc catccttatc gaggagcacg
      601 ttgtcgccaa ccgccctgcg gatcacttca agcccaattc gctgcgcctc gagcgcggtg
      661 gtgttcggcc ggtagaagaa accttccact gctgcagcat ccatgaaatc cagcttcagg
      721 aagcggactc cccattcgtt cacgagggtg gtgtaggtct gggtcagata agcctgcgcg
      781 cctggattgg tggtgtcgag aacatacagc tcgtcgaaag tgccgatcct gccgatgtgg
      841 atcgggtcgc cctgcagatt gtgcacgagc cagtctttgt ggtgttcata gacccacgag
      901 cgctctgaaa cctcgaacgg cgcgacccac aggccgaagg tgaggcccag tcctcgaacc
      961 cgaccgccta cgaaggccat tccgtggggg taggcctttc catccgcggt ggcgtactca
     1021 ccgcgggcga actgatagcc ctcatcgacc tgaaaatagc gatagcccag aggcttcagg
     1081 ttttgcgcca gccattcggc gttggtgagg gtcgttcctt cggagacgcc gtagtagtag
     1141 gcggtccagc tccaccagcc catcagggtt ggcgcctgaa cccgcgcatg ccgcagcact
     1201 cggattgcct tgccataatc ttcgagctgg gcatggtagt cggagccgat ggagaacgcc
     1261 aggcgctcgg aactgaggac ttcaccaggc gccacgtgca gccgcagggg aaccacattg
     1321 gcggagccgt agcgggtggc ccactgctcc tcacgaagag cctcgttggt gcccgtgtcc
     1381 tcagcatcga acgacaggac acgcgggttc ggcttcgctg agctttgcaa atggaagacg
     1441 gtgagaagcc gatctgcgga gagtgcgccg aggaagaggc tgagcccgct gttccggttg
     1501 tagatgagct ggcttccaaa gccgcggtgg atgcgatcct cggcatcccc gagatccatg
     1561 atcttcaact gaggcgtgtc ttcgctgaag ctgtcggaga ggacgcgatc gtcggcatcc
     1621 ggtccgttca actgcacacc ggcgccatgc acgcttttga tgacatgaat agagcgaatc
     1681 tcgat
//
LOCUS       DASZYN010000374         2907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_724124, whole genome shotgun sequence.
ACCESSION   DASZYN010000374 DASZYN010000000
VERSION     DASZYN010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724124"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VHE33_09510"
     CDS             <1..319
                     /locus_tag="VHE33_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97733.1"
                     /translation="PAEALLAAVVQKGLEHDAGRIRYIQIGNSAGPNISLAAAALRSS
                     GLELYGSGFGSVSMDKLFASLRAILASAAKEPFQFKCTTAPLRDVEKLWNSKEEARLV
                     FVP"
     gene            complement(328..633)
                     /locus_tag="VHE33_09515"
     CDS             complement(328..633)
                     /locus_tag="VHE33_09515"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HVT97734.1"
                     /translation="MDKQHFFFKLIPPRTTFPGDASAAELALMKQHADYFAGLFSGGK
                     VLLYGPVQSPAGAFGIAILEAENQAQAREIAANDPSVLGGLNRFEVAPMRVSAARAK"
     gene            805..>2907
                     /locus_tag="VHE33_09520"
     CDS             805..>2907
                     /locus_tag="VHE33_09520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT97735.1"
                     /translation="MQNLLQDLRFSARTLRNSPGFAITAILTLALGIGAVTSIFSVVD
                     SVLLKPFAFASPDRLVMLHEYLPDLASSPLPDNPNHFLNWQSNAKTFSGLCIMNNSSS
                     SISVGTDHPELVSGLSVEPNFFDVLGIHPILGRGFLPIEGTKGHNSEVVLSWNAWQRY
                     FHGDPHAIGQTLRDGGKPETVIGVLPRDFSFPRVSELYGAAQTTSNPYELFHPLFLDI
                     DQRDDEGDYNFMALGRLRTGIPVEQARTELATLQSAFNKAHHLHTNPTVLVFPMITEV
                     AGRISTALWLLLAAVGAVLLIGCVNLANLQLARAVSRERELAVRAALGAARDRLVSSA
                     LADSLVLAIMGGTLGILLSFAGVQFFIAAAPKSLPRISAVHISWPVLLAAAGLSILTA
                     LFFGLLPALRSMRVDPQSALQTSSTRVSSGRESQRTRHLLVAAEVACTVALLIVTGLL
                     VRSFSTLLNQNRNFDASHVTIARAFLYAPQYGNEVDKNQAARSGFVDRALAALAQIPG
                     VQSTAITSELPLAGDTWIDGVTRPDHPVPPGHEPDANIRWISPGYATTLRIPVVSGRD
                     LTEDDKNHPTNVLISQDAARSIFPDTNPIGHIFHLNDKTDFKVVGVLADARVNQLDHT
                     APMIYIPYWQQPWWRFTFLVRSPQSASALATSMRRAIWSVDPEVAITNLKSLDEQVSD
                     SVSNQRFQTLLLSSFGGAA"
BASE COUNT          491 a         1082 c          768 g          566 t
ORIGIN      
        1 gcccgcggag gctttgctcg ctgctgtggt ccagaagggc ctcgagcacg acgccggccg
       61 catccgttac atccagatcg gcaacagcgc cgggcccaac atctcgctcg cggctgcggc
      121 cctccgcagc tccggacttg agctctatgg cagcggattc ggcagcgttt ccatggacaa
      181 gctcttcgcc tcgctgcgcg ccatcctcgc ctccgcggcg aaggagccat tccagttcaa
      241 atgcacgact gcgcctctgc gcgacgtcga gaagctctgg aactcgaagg aagaagcccg
      301 gctcgtcttc gtgccctgag tcgcgcgcta ctttgcccgt gccgccgaca ctcgcatcgg
      361 cgctacctca aaccggttca gcccgcccag caccgaagga tcgttcgcgg cgatctccct
      421 cgcctgcgcc tggttctcgg cttccagaat cgcgatccca aacgcccccg ccggcgactg
      481 cactggcccg tagagcaaca cttttccccc agagaacagg ccggcaaaat aatccgcgtg
      541 ctgcttcatc agcgccagtt ccgcggcgct cgcatcgccc ggaaaggtcg ttcgcggcgg
      601 aatcagtttg aagaagaagt gctgcttatc catggtgcag ccatcttaac ccgcttccag
      661 cttccacctg ctcctgacta cgggctattg gttcctggct gccccccgtc cacccgtcac
      721 tttcagcgaa caaacccctt tgcgcgggcg tatcgcctgg gagatgatgg gcaccatctg
      781 ctgagaccgt ccccgagagg ccgcatgcaa aatcttctgc aggacctgcg cttcagcgcg
      841 cgtaccctcc gcaactcgcc cgggtttgcc attaccgcga tccttaccct cgccctcggc
      901 atcggtgcgg tcacctccat cttctcggtt gtcgactccg tcctgctgaa gcccttcgca
      961 ttcgccagcc ccgaccgcct cgtgatgctg cacgagtacc tccctgacct ggcttccagc
     1021 ccgctgccgg acaaccccaa ccacttcctc aactggcaga gcaacgcgaa gaccttctcc
     1081 ggcctgtgca tcatgaacaa cagcagctcc agcatttcgg tgggcaccga tcaccccgag
     1141 ctggtcagcg gcctgagcgt cgaacccaac ttcttcgacg tcctcggcat tcatcccatc
     1201 cttggacgcg gtttcctgcc catcgaaggc accaaggggc ataacagcga agttgtgctc
     1261 tcctggaatg cctggcagcg gtatttccat ggcgatcctc acgccatcgg gcaaactctg
     1321 cgcgacggcg gcaagccgga aacggtcatc ggcgttttgc cccgcgattt ctcttttccg
     1381 cgcgtgagtg aactgtacgg ggcagcccag acgacgagca acccatacga gctgttccac
     1441 ccgctgttcc tcgatatcga ccagcgggac gacgagggcg attacaactt catggcgctg
     1501 ggccggctcc gcaccggcat tccagtggaa caggcgcgaa ccgaactcgc cacactgcag
     1561 tccgccttca ataaggcgca tcacctccac accaacccca ccgtcctcgt ctttcccatg
     1621 atcacggaag tcgcgggccg catcagcacc gcgctgtggc tgctgctggc cgcggtggga
     1681 gccgtcctgc tcatcggctg cgtcaatctc gccaatctgc agctggctcg tgctgtttca
     1741 cgcgagcggg aactcgcggt ccgtgccgcc ctcggcgccg cacgcgatcg cctcgtgagt
     1801 tccgcactcg ccgattcgct tgtcctcgcc atcatgggag gcacgctggg catcctgctt
     1861 tcttttgccg gcgttcagtt cttcatcgcc gcagccccga agagcttgcc ccgcatcagc
     1921 gccgtccata tctcctggcc tgtcctgctg gccgccgccg gtctctccat ccttaccgcg
     1981 ctcttcttcg gactcctgcc cgccctgcgc tccatgcgcg tcgacccaca atcggcgctg
     2041 caaaccagct ccacccgcgt ttccagcggc cgcgagagcc agcgcacccg ccatctgctg
     2101 gtcgccgccg aggtcgcctg cacggttgca cttctcatcg tcaccggatt gctcgtccgc
     2161 agtttctcga ccctgctcaa tcaaaatcgc aacttcgacg cgagccacgt cacgattgcc
     2221 cgcgcgtttc tgtatgcgcc acagtacggc aacgaggtcg acaagaacca ggccgcccga
     2281 tccggctttg tcgatcgcgc tctggctgca ctggctcaga ttcccggcgt gcagtcgacc
     2341 gcgatcacct ccgagcttcc tctggccggg gatacctgga tcgacggtgt gacgcggccc
     2401 gatcaccctg ttccgcccgg ccatgaaccc gacgcgaata tccgctggat cagccccggc
     2461 tacgcgacca cgctgcgcat tccggtcgtc tccggccgcg acctgaccga ggacgacaag
     2521 aaccacccca ccaacgtcct catctcccag gacgccgcgc gcagcatctt cccggacacg
     2581 aatcccatcg gccacatctt ccacctcaac gacaaaacgg acttcaaagt cgtcggcgtt
     2641 ctggccgatg cgcgcgtgaa tcagctcgat cacaccgcgc ccatgattta catcccgtac
     2701 tggcagcagc cgtggtggcg cttcaccttt ctcgtccgca gtccgcagtc ggcctccgcg
     2761 ctggccactt cgatgcgccg cgcgatctgg agtgtggatc cggaagtggc catcaccaac
     2821 ctcaaatccc tggacgagca ggtttccgat tccgtttcca atcagcgctt ccagacactg
     2881 cttctctcca gcttcggcgg cgctgcg
//
LOCUS       DASZYN010000375         3520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1235797, whole genome shotgun sequence.
ACCESSION   DASZYN010000375 DASZYN010000000
VERSION     DASZYN010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235797"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VHE33_09525"
     CDS             <1..391
                     /locus_tag="VHE33_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVT97736.1"
                     /translation="PWSPHMSAWSGDTLPVKYLKEATPLERQGAVIFQAKQCRNCHSI
                     GGHGGERGPALDSIATRMTVDQMRYKVVTGGGNMPAYGKNLSPPEIEALVSFLETLHP
                     ANQPPAFDASQQDATARTAPQPVKAKP"
     gene            609..1463
                     /locus_tag="VHE33_09530"
     CDS             609..1463
                     /locus_tag="VHE33_09530"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HVT97737.1"
                     /translation="MNQDALFTQWDVPWAVTAALAVTAAIYVRGWLQIRRTRPEMFPR
                     WRLAAFLSGILAIFVAVASPLDTFSETLLFMHMAQHFVLMSIAPPLIVLGAPVVPMLR
                     GLPRGAIRGLRPVFRSGVFGRVGSFLTRPKVAWLAMNAAYVGWHIPKAYEFALSSENW
                     HNFEHGCFFFTSVMFWWPVIAPWPWRQNWNRWLLLPYLLLADLVNTGLSAFLCFSGRL
                     LYPSYGEVPRLFGLSALNDQIAAGAFMWVMGSTVFLIPAMGITVMLLSPGRRRLPANR
                     REAMAGGR"
     gene            complement(2253..3272)
                     /locus_tag="VHE33_09535"
     CDS             complement(2253..3272)
                     /locus_tag="VHE33_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HVT97738.1"
                     /translation="MATATPAQVTPERIMQMAWGYVPPLVLEAAIRHHVFDVLDSGPK
                     DIWEVQKATGASARGLAAIMDALVGLNFLSKDEQNRFALTPESSTFLVSTKTTFFGGM
                     IKHCSEQLIPRWLNLNEVVATGKPVTPVNEEKSGGEFFHEFVLDIFPMSYAPAQALAA
                     HMNYGSSAAPVRVLDLAAGSGVWGIAQAQSSPNVSVTAVDWPEVIDITKKTAAKFGLT
                     DRFSFVEGDLLDVSFGSGHNVATLGHILHSEGEARSRRLLAKTLAALSSGGTIAIQEF
                     LVNKERTGPVNGLFFAVNMIVNTQEGNTWSFEEIGAWLEEAGFINPRLLESPGPSPLI
                     LATKP"
BASE COUNT          597 a          826 c         1355 g          742 t
ORIGIN      
        1 gccgtggagt ccgcacatga gcgcgtggag cggagacaca ctgccggtga agtatctgaa
       61 ggaagcgacg ccactggagc gacagggggc ggtgatcttt caggcgaagc aatgccggaa
      121 ctgccactcg atcggggggc atgggggcga gcgcgggccg gcgctggact cgattgcgac
      181 acggatgacg gtggatcaga tgaggtacaa ggtggtgacg ggcggcggga atatgccggc
      241 gtacgggaag aatctgtcgc cgccggagat tgaggcgctg gtgagttttc tggagacgct
      301 gcatccggcg aatcagccgc cggcgttcga tgcgtcgcag caggatgcga ctgcgaggac
      361 agcgccgcag ccggtgaagg cgaagccgtg aggaagccct gaagggcagg gtttggagtt
      421 tagagtttgg ggtttcggcg cgggcgttcg cacaacccaa tcgcgccttt cacgttttta
      481 ctgcgaaggt gggttcgtgc cggagcggcg cattggcttc cacatccgca ctgacggcag
      541 atgcggcgcc cacgtactcg agtttttcca cgatagagcg gtaaggcgaa ggacatgaac
      601 ggaaacggat gaatcaggac gcgctgttta cgcagtggga tgtgccgtgg gcggtgaccg
      661 ctgcgctggc ggtgacggcg gcgatttatg ttcgcggatg gttacaaata aggcgaacgc
      721 ggccggagat gtttccacga tggcggctgg cagcgtttct gagcgggata ttggcgatct
      781 ttgttgctgt tgcgtcgccg ctggacactt tcagcgagac gctgctgttc atgcatatgg
      841 cgcagcactt tgtgctgatg tcgattgcgc cgccgctgat cgtgctggga gcgccggtgg
      901 tgccgatgct gcggggacta ccgcgaggcg cgattcgcgg gctgcgtccg gtgttccgga
      961 gcggcgtgtt cggacgggtg ggatcgttcc tgacgcggcc gaaggtggcg tggctggcga
     1021 tgaacgcggc atacgtgggc tggcatattc cgaaggcata tgagttcgca ctgtcgagcg
     1081 agaactggca caacttcgag catgggtgct tcttcttcac gagcgtgatg ttctggtggc
     1141 cggtgattgc gccgtggccg tggcgacaga actggaatcg ctggctgctg cttccctact
     1201 tgctgctggc ggatctggtg aacaccgggc tgtcggcgtt tctgtgtttt tcagggcggc
     1261 tgctgtatcc gagctatggg gaggtgccgc ggttgttcgg gctgagcgcg ctgaacgatc
     1321 agatcgcggc gggggcgttc atgtgggtga tgggatcgac ggtttttttg attccggcga
     1381 tgggcatcac ggtgatgctg ttgtcgccgg ggaggcgacg tctgccagcg aaccggcgcg
     1441 aggctatggc cggcggccgg tagccacagc ggtgacgatt ctgtttgtgt ttggtgaggt
     1501 ccccacgatt ccgccgggtc ggctctttgg ggacgatggt gcgtcttgtg ggaggacgaa
     1561 agagtgcggg aagaggacgc tttttcccgg aatgggaagg gtccttccgg ggacggtact
     1621 ttcggctggc ggagcggctg aaatcctttg acgacctgga tggggtgaac ctggcgcttc
     1681 agttttttgg gggtgcattt tcccggaatg ggaaaggtcc ttccggggac ggtactttgg
     1741 gctgagggag aggctgaaat gctttgacgg cctggatggc ggagagtctc ctgcttgctg
     1801 tggggtgaac ctggcgcttc agttttttgg ggtgcttttt cccgaaattg aaacgagcct
     1861 tcccggaatg ggaaggggtc ttccggggac ggtactttcg gctgggggag agactgaaat
     1921 cctttgacgg cctggatggc gggggagtct tctgcttgct gtggggtgaa cctggcgctt
     1981 cggcgcacgg gggtcgtggt tctgcggttc tgctcgagga cggaacggtt gcggactcgg
     2041 cccctgggga actcggattt gcaggagttc agggattggg aattggagtt tacgctccgt
     2101 ttcgagcccc atgtctcagg agtcatcgca tgggtatcgc gggatgcaga ctgctccagc
     2161 gcgagatccc cgcgggcgcg tttgcacgcg cccgcagtat ggcatgcgtg aagaagaatc
     2221 ctccggcaga gccgggggcc tcctctgttt agctagggtt tggtggcaag gatgaggggt
     2281 gagggaccgg gggattcgag gaggcgggga ttgatgaagc cggcttcttc gagccaggcg
     2341 ccgatttcct caaacgacca ggtattccct tcctgcgtat tcacgatcat gttcacagca
     2401 aaaaagagac cgttgacggg gccggtgcgc tccttgttga cgaggaattc ctgaatggcg
     2461 atggtgccgc cgctcgagag cgcggcgagc gtcttcgcca ggagccttcg gctccgggct
     2521 tcgccttcgc tgtgcaggat gtgaccgagg gtagcgacgt tgtggcctga accgaaggag
     2581 acgtcgagga gatcgccctc gacgaaggaa aagcggtcgg tgaggccgaa tttcgcggcg
     2641 gttttctttg tgatgtcgat gacttcgggc cagtcgacgg cggtgacgct gacgttggga
     2701 gagctttggg cctgggcgat gccccagaca ccggagccgg cggcgaggtc gaggacacgg
     2761 acgggcgcgg ccgaactgcc gtagttcatg tgggcggcga gggcctgggc gggggcgtag
     2821 ctcatgggga agatgtcgag gacgaattcg tggaagaatt cgccgccgga tttttcctcg
     2881 ttcacgggcg tgacgggttt gccggtggca acgacttcgt tgagattgag ccagcgggga
     2941 atgagctgtt cggagcagtg cttgatcatg ccgccgaaga acgtagtttt ggtgctgacg
     3001 aggaaggtgg agctttccgg agtgagggcg aagcggttct gctcgtcttt ggagaggaag
     3061 ttaaggccga cgagggcgtc catgatggcg gcgaggccgc gggcggaggc gccggtggct
     3121 ttctgaactt cccagatgtc tttggggccg gagtcgagga cgtcgaagac gtgatgtcgg
     3181 atggcggctt cgaggacgag cggcgggaca tagccccagg ccatttgcat gatgcgttca
     3241 ggggtgactt gcgcaggagt agcggtagcc atgagtgttc ctcatttccg ggggagcgga
     3301 gaatatcgaa tcgtgaccgc tggggagcgt ggcgatgggg aggagtttag cagctttgga
     3361 ggttgactcg tgcgaaggga ggggggacaa aagaagggct cgcgccagga ggcgagccct
     3421 tgtgggggag gaagtgattt aacgcctgtc cccgggttag agggtcaggt gtgggttaga
     3481 cggaaaggag gcgcgggcgt gatcggggct tggaggcccg
//
LOCUS       DASZYN010000376         6600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1109432, whole genome shotgun sequence.
ACCESSION   DASZYN010000376 DASZYN010000000
VERSION     DASZYN010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109432"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /gene="hemW"
                     /locus_tag="VHE33_09540"
     CDS             complement(<1..812)
                     /gene="hemW"
                     /locus_tag="VHE33_09540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HVT97739.1"
                     /translation="MLGIYLSIPFCRSKCTYCNFSSGVFPASYHDRYISCIEKDLSRI
                     RASAKKWGAQLPQAVDSIYLGGGTPSLLSPELFHRLFTALRREFAVLPTAEITVECAP
                     GQIEPAVLEALVECGVTRVSFGVQSFVDSEAKATGRLHNRAAALEDIRRVQVAGISNV
                     SVDLIAGLPGQTMNSWYESLQVLADSGVDHASIYMLEVDEDSRLGREMLVNGGRYRAN
                     EVPPDDRIAEFYTEAIGFLAGHGLAQYEISNFARTGCESRHNLKYWRRQPYLG"
     gene            1020..1364
                     /locus_tag="VHE33_09545"
     CDS             1020..1364
                     /locus_tag="VHE33_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97740.1"
                     /translation="MKSILRALALTGIVGFGAAAGAQAQTRFYVGIGTSAVAAIPPCP
                     GEGYVWTAGYYDSYGWVPGQWVYRGYDRDNYYRARVDWDHRYYDHDRDRDRRYDRDRH
                     DRDDHYRGHDRH"
     gene            1554..1772
                     /locus_tag="VHE33_09550"
     CDS             1554..1772
                     /locus_tag="VHE33_09550"
                     /inference="COORDINATES: protein motif:HMM:NF025731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection immunity protein"
                     /protein_id="HVT97741.1"
                     /translation="MAGLLGVAIYFLPSIVAAARQTHNVTGIFLLNLFLGWTGIGWII
                     ALLLAMGSTPSYCYPPPWYYPPPPRRWY"
     gene            1968..2675
                     /locus_tag="VHE33_09555"
     CDS             1968..2675
                     /locus_tag="VHE33_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97742.1"
                     /translation="MRTLRNALFLGLLALPLALAKPAQAQVAIGIGIGPEVAYPGYYD
                     IAGPPVCDYGYYPYYPYTCAPYGYWGPDWFAGGVFIGAGPWYHGWYGRPWGWGYGHVG
                     YYGGWGRGYYGYRGWGGYHGYGRGYHGYAGGYHGYAGGTRGFNGRGYGGAYHGPYTNG
                     FRGGAGAYRGNSGGFRGGAAGGFHGGAAGGFRGGAGGGFRGGMAGGFHGGGGGFHGGG
                     GFHGGGGGGFHGGGGHR"
     gene            complement(2754..3881)
                     /gene="murG"
                     /locus_tag="VHE33_09560"
     CDS             complement(2754..3881)
                     /gene="murG"
                     /locus_tag="VHE33_09560"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HVT97743.1"
                     /translation="MRDTLSAQTGPARVLIAGGGTGGHIIPALAIADELKARHGADLL
                     FVGTARGLESRLVPQAGYSLELIRVGQLNRVSLMTRLRTLVDLPLGFWQCVRLLRHFR
                     PDVVIGVGGYASGPVMGAAILLRIPTLAFEPNAVPGLANRLIGSRVRAAAVNFEPAAR
                     YFRNAHVTGIPVRNEFFRLAARPAGAIPHLLVFGGSQGARTLNAMMPKIAASLLNAIP
                     GLTILHQAGARHAEATLQAYQESGAPPDRWQVAPFLDDMPRRFEAADLVLARSGASTV
                     AELAAAGKPAVLVPLPTAADDHQRKNAEVMAAAGAARVLLEPDLTPAQLEGTLSELLA
                     APAELQRMAENARTLAHPDASERIAAIAMALARRDFRKEPV"
     gene            complement(3891..4982)
                     /gene="ftsW"
                     /locus_tag="VHE33_09565"
     CDS             complement(3891..4982)
                     /gene="ftsW"
                     /locus_tag="VHE33_09565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HVT97744.1"
                     /translation="MAKRVGVDKWLYFAAIVLVVIGLAMVFSASAVMSGERFGSPYYF
                     VVRQSLWAVFGLLAMTLLMRVPYSNYNKPTVVFPVVGVTIILLLAAFFMHDSHNTHRW
                     IRFSVLSFQPSEIAKPALVLFLAWFLQNRMHLMDDWRNTLIPAALPSLIFIALILKEP
                     DLGTALVCAGVTALMLWLAGMHPKYLGYAAGAAAPVLYIMLFAVKWRRDRMLAFINPE
                     SDPLGKGFHIIQSLIAVGSGGFHGVGYMDGRQKLFYLPEPQTDYIFANVAEELGLIGA
                     LIVVGLFVFIGWRGMRAAILSRDPFARFVAFGITATILIQAFFNVSVVLALVPTKGIT
                     LPFVSYGGTSLFIMLVLVGVLLNITREVD"
     gene            complement(5000..6379)
                     /gene="murD"
                     /locus_tag="VHE33_09570"
     CDS             complement(5000..6379)
                     /gene="murD"
                     /locus_tag="VHE33_09570"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HVT97745.1"
                     /translation="MEIKGKKVLVVGLGKSGLAAALFLRRQGAQVTVSDVRSAEELTK
                     EIPALIEEGIAVEAGGHGLLTFRRQDLIVVSPGVPVDTPELAQVQKFGLPIIGEVELA
                     AHYLRGKVLAITGSNGKTTTTTLCGEILKAGGLQVQVGGNIGVPVIALVDGSRDDGWS
                     VLEVSSFQLETTHEFRPQIAVILNITPDHLDRHGTFENYVAAKERIFAQQTVDDALVL
                     NADDDVASRAAVRAKARVFWFSQKKIVRQGAFVHEGTITWRASEQAAPEPVVKVSEIP
                     LKGAHNIENVLAAVCAARLAGVEAAAIRKAVVEFHAVEHRLEFVAKINGVDYYNDSKA
                     TNVDASMKAIAAFPGGIHLILGGKDKNSDYRQMRALLQERVKAVYTIGAAAEKIHTHI
                     EGAVPIVSAGTLDAAIAKAAAAAQPGEIVLLAPACSSFDQFENYEHRGRVFKETVLAR
                     RGLAALSAT"
BASE COUNT         1319 a         2120 c         1949 g         1212 t
ORIGIN      
        1 ccgagatagg gctggcggcg ccagtatttc aggttgtgcc gcgactcgca gccggtgcgg
       61 gcaaaattcg agatctcgta ttgcgccagg ccatggccgg caagaaagcc gatcgcttcg
      121 gtataaaact cagcgatgcg atcgtcgggg ggaacttcgt tcgcgcgata acgtccaccg
      181 ttcacaagca tttcgcggcc cagccgcgaa tcttcgtcga cttccagcat gtaaatgctc
      241 gcgtgatcga cgcccgaatc cgcaagaacc tgcagggatt cataccacga gttcatggtc
      301 tggcccggca gaccggcgat cagatcgaca ctaacattgg aaatgcccgc aacctgcacg
      361 cggcgaatat cttcaagagc cgccgctcgg ttgtgcaggc ggccggttgc cttcgcttcg
      421 ctgtccacaa aggactgcac gccaaagctg acgcgcgtga cgccgcattc caccagagcc
      481 tcgagcacgg ccggttcaat ttgcccggga gcgcattcaa cggtgatctc agccgtcggc
      541 agcacggcga attcacggcg caatgcggtg aacagccggt gaaacaactc cggtgacagc
      601 aacgacggcg ttccaccgcc caggtagatg ctgtcgaccg cctgcggcaa ttgcgcaccc
      661 catttttttg cgctcgcgcg gatgcggctg aggtccttct cgatgcagga aatatagcga
      721 tcgtgataac tggcgggaaa aactcctgaa gaaaaattgc agtaggtgca cttggaacgg
      781 caaaacggaa tggagagata aatcccgagc atttccatat gtttctattg tcccactgcg
      841 atcgaagatc gcctatgcgg tgacaatcta ttcatcttga cgagccgcgg gcggtgtgcc
      901 gcaacaattc gcgcaaagca gcgttcactt catgcagaga cgatccggtg caaagagggc
      961 cggatgagag gttcacaact tcgaaagcaa ttcgaagcga acccgaggag gtctcgaaaa
     1021 tgaaaagcat ccttcgcgca ctcgcactca ccggcattgt tggttttggc gcagccgccg
     1081 gagcacaggc tcagacaagg ttctatgttg gcatcgggac ctcggccgtc gcggccattc
     1141 caccttgccc gggtgaaggg tacgtgtgga cagcaggcta ctacgacagc tatggctggg
     1201 ttccgggcca gtgggtttat cgcggatacg atcgggacaa ctactatcgg gcccgggtgg
     1261 actgggacca ccgctactac gatcacgacc gtgaccggga tcgccgctac gaccgcgacc
     1321 ggcacgatcg cgacgaccac taccgcggac acgaccggca ctgagtgaac aggatgcggg
     1381 ccgtgtcccc aaaaaccggc ccatgcgcac ccgttgaagc tccccgcttc cgggtgcgta
     1441 ccgttttcag gctgcgaaaa ttcgctcctc cctgcgcgct tcgcagcatc gctgaaggga
     1501 acaacgacaa tccctcatgc gtatcctttg cctgaaacct tgcggaggca cccatggccg
     1561 gactcctcgg tgtcgcaatc tactttctgc cctcgatcgt tgcagcggca cgccagacgc
     1621 acaacgtgac cgggatcttc ctgctgaacc tttttctggg ctggaccggg atcggctgga
     1681 tcatcgccct gcttctggcc atgggttcca cacccagcta ttgctaccct ccgccgtggt
     1741 attacccacc gccgccccga cgctggtatt gaagaacgac aaccgagcgc taccagccca
     1801 ttgtgaaagg gtttatcctc cgggaaacca gcgaaaagac gccgcaacat tctgcggcgt
     1861 ctaacgttag tagaggtagc cgccgacgga gtccgctgca ggaaccggga gggggcgttc
     1921 agatcgcaac gatagaaaat gagagcgatc tgaaggaggt ttggaccatg cgcacgctgc
     1981 gcaacgcttt gttcctcgga ttgctggcct tgcccctcgc cctggcgaaa ccggctcagg
     2041 cacaggtggc gattggcatc ggaatcgggc cggaggtcgc ttatcctggc tactacgaca
     2101 tcgcggggcc gccggtctgc gactacggct attaccccta ttacccctat acctgcgcgc
     2161 cctacggtta ctggggccct gactggtttg ccggcggcgt cttcattggc gccggcccgt
     2221 ggtaccacgg ctggtatggc cgtccgtggg gttggggcta cggccacgtt ggatactacg
     2281 gcggctgggg acgcggctat tacggatacc ggggctgggg cggctatcac ggctacgggc
     2341 gcggctacca tggctacgcc ggcggatacc acgggtatgc cggaggcacg cggggcttca
     2401 atggtcgcgg ctacggcggc gcctatcacg gcccgtacac caatgggttc cgtggcggag
     2461 ccggcgctta tcgcggaaac tccggtggat tccggggtgg cgccgcaggt ggattccatg
     2521 gcggggccgc tggcggcttc cgtggtggag cgggcggcgg gttccgcggc ggtatggctg
     2581 gtggattcca tggcggtggt ggcggattcc acggcggcgg cggatttcat ggtggcggag
     2641 gcggcggctt ccacggcggt ggtggtcacc ggtaattctt cgctgcccaa cactaaaagg
     2701 cccgcttttc ggagcgggcc ttcgtgtcac tggatcgtgc cttctggctg tgcctaaacc
     2761 ggctccttcc gaaaatccct ccgcgccaaa gccatcgcga ttgcggcgat ccgctctgac
     2821 gcatccggat gcgcgagcgt tcgggcgttt tcggccatcc tttgcaactc agccggtgcc
     2881 gcgagcagtt cgctgagggt cccctccagt tgcgccggag tcagatcggg ttcaagtagc
     2941 acacgcgcag ccccggctgc cgccatcacc tcggcattct ttctttgatg gtcatcggcg
     3001 gcagtcggca agggcaccag taccgccggc tttccggcag cagcgagttc ggcgaccgtc
     3061 gatgccccgc tgcgtgcgag caccaggtcc gccgcctcaa aacggcgcgg catgtcatcg
     3121 aggaatggcg ccacctgcca ccgatccggc ggagcaccgc tttcctgata cgcctggagc
     3181 gtcgcctcgg cgtggcgcgc tcccgcctgg tgcaggatgg taagccccgg gatcgcgttc
     3241 agaagagaag cggcaatttt cggcatcatc gcattcaagg tgcgcgctcc ctggctgccc
     3301 ccgaagacca gcagatgcgg tatggctcct gcgggcctgg ctgcgagccg aaagaactca
     3361 ttccgaaccg gaatcccggt gacatgcgca ttgcgaaaat atcgtgccgc aggctcgaag
     3421 ttcacggcag ccgccctgac ccggcttccg atcagccgat tcgccagacc gggcacagcg
     3481 ttgggctcaa aggccagcgt cggaatcctc aaaaggatcg ccgcgcccat caccggaccg
     3541 gaagcgtagc ctccaacacc gatcaccacg tccggccgga aatgccgcaa cagccgcaca
     3601 cactgccaga aacccagggg gagatccacc agcgtgcgca gcctcgtcat gagggagacg
     3661 cgattcagct gtcctacgcg aatcagttcc agggagtagc ccgcctgcgg caccagtctc
     3721 gattccagtc cgcgcgccgt gccgacgaag aggaggtcag ctccatgccg ggccttcagc
     3781 tcatccgcaa tggcgagtgc cggaatgatg tgcccgcccg tgccgccacc ggcgatcagg
     3841 acgcgcgcgg gtccggtctg tgccgagagc gtgtcccgca ctctgtccct tcagtccact
     3901 tcccgggtga tattcagcaa cacgcccacc agcaccagca tgatgaacaa ggacgttccg
     3961 ccgtaggaga caaagggcag cgtgatgcct ttggtcggca ccagcgccag cacgacgctg
     4021 acgttgaaga aagcctggat cagaatcgtc gccgtgatgc caaaggcgac gaagcgggca
     4081 aagggatccc gggagagaat cgccgcccgc atgccgcgcc acccgatgaa gacgaacagg
     4141 cccaccacaa tcagcgcgcc aatcagaccc agttcttccg ccacgttggc aaagatatag
     4201 tccgtctgcg gctccggcag atagaacagc ttttgacggc catccatata acccacgcca
     4261 tggaacccgc ccgagcccac agcaatgagt gactgaataa tgtggaagcc cttgcccagc
     4321 ggatcggatt ccgggttgat aaaagccagc atgcggtcgc ggcgccactt gacggcaaac
     4381 agcatgatgt acagcaccgg cgccgctgcc cccgcagcgt aacccaggta cttcgggtgc
     4441 attccggcca gccacaacat caatgcggtc acgcccgcgc acaccagcgc ggttcccagg
     4501 tccggctctt tgaggatcaa agcaataaag atcaggctgg gcaacgccgc gggaatcagc
     4561 gtgttacgcc agtcgtccat cagatgcatg cggttctgca gaaaccatgc aagaaacagc
     4621 accagggccg gtttcgcgat ctcggacggc tgaaaagaga gcacgctgaa gcgaatccag
     4681 cggtgggtgt tatgcgagtc gtgcatgaag aatgccgcga gcagcaggat gatcgtgacc
     4741 cccacgaccg gaaagaccac cgtgggcttg ttgtagttgc tgtacggcac gcgcatcagc
     4801 agtgtcatag caaggagtcc gaaaacagcc cacagcgact ggcgaacgac aaagtagtac
     4861 ggcgagccga agcgctcccc ggacatgaca gccgacgcgc tgaagaccat cgccaggccg
     4921 atcacgacca gcacgattgc cgcgaagtac aaccacttgt cgacgccgac tcgtttcgcc
     4981 ataagactcg tttctgtacc taggttgccg aaagtgcggc cagaccgcgc cgcgccagaa
     5041 ccgtctcctt gaacacgcgt ccccgatgct cgtagttctc gaactggtcg aagctggaac
     5101 aggcaggcgc gagaagcacg atctcgcccg gttgtgccgc agcagcggct tttgcaatcg
     5161 ctgcatccag cgttcccgcg ctcacaatgg gaacggcccc ttcaatgtgc gtgtgaatct
     5221 tctcggccgc cgccccaatg gtgtacacgg ctttgacccg ctcctgcagc agcgcgcgca
     5281 tctgccggta gtcggagttt ttgtccttgc cgccgaggat caaatggatc ccaccgggaa
     5341 aagcggcaat ggccttcatc gacgcgtcca catttgtcgc cttcgagtcg ttgtagtagt
     5401 cgacgccgtt gatcttcgcc acaaactcca gccgatgctc gaccgcatgg aattccacca
     5461 ccgctttgcg gatcgctgcc gcctccacgc ccgccaatcg cgccgcgcat accgctgcca
     5521 ggacgttctc gatgttgtgc gcacctttaa gcggaatttc gctcaccttc accaccggct
     5581 ccggagcagc ctgttccgaa gcgcgccacg tgatcgtgcc ttcatggacg aaagctccct
     5641 gccgcacgat ctttttttgg ctgaaccaga acacccgcgc ttttgcacgg actgctgctc
     5701 tggacgccac atcgtcgtcg gcgttcaaca ccagcgcatc gtcgaccgtc tgctgggcga
     5761 aaatcctctc cttcgccgcg acgtagttct cgaaagtccc gtgacgatcc agatgatccg
     5821 gcgtgatgtt caggatcacg gcaatctgcg gacgaaattc gtgcgtcgtc tccaactgga
     5881 agctcgagac ctccagcacc gaccagccgt cgtcccgaga tccgtcgacc agcgcgatca
     5941 ccggaacccc gatgttgcca ccgacctgaa cctgcagccc acccgccttg agaatctctc
     6001 cacaaagagt cgtcgtcgtg gtcttgccat tggagccggt gattgccagc accttcccgc
     6061 gaagatagtg cgccgccagc tcgacttctc cgatgatcgg caaaccgaat ttctgaacct
     6121 gggccagctc aggtgtatcc accggaacgc ccgggctcac gacgatgagg tcctgccgcc
     6181 ggaacgtgag cagcccatgg ccgcccgcct cgaccgcaat gccttcttcg atgagcgccg
     6241 gaatttcttt cgtcagctct tcggcgctgc gcacgtcgga aaccgtcacc tgcgcgccct
     6301 gacgccgcag aaacagcgcc gctgccagcc ccgacttgcc aagaccgacg accagtactt
     6361 tttttccttt gatttccatc cgcaactcgc gcgccgggtg gcttcattgt gtcacgccgg
     6421 ctctgtcctt tatcgcagct tgagggtggt gagtgcaaag agtgcaaaca ccagcgccag
     6481 gatccagaag cgcgcgatca cttttgactc cgaccagccc agcagctcga agtgatgatg
     6541 caacggcgcc atgcggaaga tgcgcttgcc gcctcgcagc ttatagctcc ccacctgcag
//
LOCUS       DASZYN010000377         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_978781, whole genome shotgun sequence.
ACCESSION   DASZYN010000377 DASZYN010000000
VERSION     DASZYN010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978781"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..319)
                     /locus_tag="VHE33_09575"
     CDS             complement(140..319)
                     /locus_tag="VHE33_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97746.1"
                     /translation="MLTPSHFTAVLLFATCTSVVFGVTQRSTPRGMVRYGIYCWVLFI
                     ASVIVASWLMWIIRR"
     gene            complement(319..723)
                     /locus_tag="VHE33_09580"
     CDS             complement(319..723)
                     /locus_tag="VHE33_09580"
                     /inference="COORDINATES: protein motif:HMM:NF042755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HVT97747.1"
                     /translation="MATKEQTPAPAKSQGFVYSALILGWLVPGAGHLLTRHWIRALVL
                     FLAITAMFSLGLAMQAKVYVPNTGDPLDMLGFAGDLGTGLLYFIARIFSLGQSTVQLV
                     TADYGTKFVVVAGLLNFVSAVDAHNLRIGRKV"
     gene            complement(781..1953)
                     /locus_tag="VHE33_09585"
     CDS             complement(781..1953)
                     /locus_tag="VHE33_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HVT97748.1"
                     /translation="MDLDAIQAALRGARLDAWLFYDHHHRDPLAAAILGLPDHGLVSR
                     RWYYVIPAEGEPRKLVHRIEADKLDSLPGSKGVYSSWQELETRLDAMLAPWNRIAMQY
                     SPRNSIMYVSMVDAGTIELIRSMGKDIVSSADLVSQFQAVLTPEQIDSHYAAQKALDE
                     ILVQGFSYIGSRARSGSPATEYDMMLWFREAMDRAGLTTDHGPNVSIDENSSNSHYEP
                     SAESSRPIVSGSFLLIDIWARLNRPGTCWYDITWTGVVNREPSEQERVVFAVVRDARD
                     AAIQAVRTAFTDGAHPIAGFQADDAARNVISNAGYGPYFTHRTGHNIGAELHGSGAHL
                     DNLETHDERRILPFTCFSVEPGIYLPEFGVRSEVNMITSRDNAVVTGRIQRDLVRI"
     gene            2057..2341
                     /locus_tag="VHE33_09590"
     CDS             2057..2341
                     /locus_tag="VHE33_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97749.1"
                     /translation="MKRFLNILKFTVKVVLVTAVAVWVGDWALFRVRAARGTAFDTVQ
                     VEEYLTTALKGNKQEYDYMGTAQVTCARAVFPHGGAPACWWVRRHRTEWE"
BASE COUNT          476 a          579 c          881 g          427 t
ORIGIN      
        1 aagccggagg gggtactgga ggtgccggaa cggccgggca gtttgtgctt tggagggaaa
       61 gaggggcgca cgctgttcat cgcggcgcgc agttcgattt acgccatcca gacgcgggaa
      121 ccgggaaagt gatgcgaagt cagcgtcgga tgatccacat gagccagctg gcgacgatca
      181 ctgaggcgat aaagaggacc cagcagtaga tgccgtagcg aaccatgccg cgcggcgtcg
      241 agcgctgggt gacgccgaag accaccgagg tgcaggtagc aaacaacagg actgccgtga
      301 agtgggaggg agtgagcatt agaccttcct cccgatgcgc aggttgtgag catcgacggc
      361 ggagacgaaa ttcagcaggc cggcgacgac cacgaacttg gtaccgtaat cggcggtaac
      421 cagctggacg gtgctttggc ccagcgagaa gatgcgggcg atgaagtaga ggagtcccgt
      481 gccaagatcg ccggcgaagc cgagcatgtc gaggggatcg ccggtgttgg gaacgtagac
      541 cttggcctgc atggcaaggc cgagcgagaa catggcggtg atggccagga agagcacgag
      601 tgcccgaatc caatggcgag tgagcagatg gcctgcaccc ggcacgagcc atccgaggat
      661 gagtgcggag tagacgaagc cctgagattt ggctggtgca ggggtctgct ctttagtcgc
      721 catgctttct atgactataa atgaacggcg cagatgagtt gctgtggccg gagcgaggcc
      781 tcagattcgc acgaggtccc tctggatgcg gccggtcacg acggcattgt cgcgcgaggt
      841 gatcatgttg acttcgctgc ggacgccaaa ttccggcaga tagatgccgg gctcgacgga
      901 aaagcaggtg aagggcagga tgcgccgctc gtcgtgagtt tcgaggttat cgaggtgggc
      961 gccgctgcca tggagttcgg cgccaatgtt gtgcccggtg cgatgggtga agtacggccc
     1021 atatccggca ttgctaatga cattgcgggc cgcgtcgtcg gcctggaaac cggcgatggg
     1081 gtgagcaccg tcagtgaagg cggtgcgcac agcctgaata gcggcatcgc gcgcgtcacg
     1141 gacgacggcg aagacgacgc gctcctgctc gctgggttcg cgattgacga caccggtcca
     1201 ggtgatgtcg taccagcagg tgccggggcg attgagacgc gcccagatgt cgatgaggag
     1261 gaacgaaccg gagacaatgg ggcggctcga ttcggcgctg ggctcgtagt gggagttgga
     1321 gctgttttcg tcgatggaaa cattggggcc gtggtcggtg gtgaggccgg cgcggtccat
     1381 ggcctcgcgg aaccagagca tcatgtcgta ttcggtggcg ggggagccgg agcgggcacg
     1441 gctgccgatg tacgagaaac cctgcacgag gatttcatcg agagctttct gggcggcgta
     1501 gtggctgtcg atctgttccg gagtgagaac ggcctggaac tggctgacca gatcggcgga
     1561 gctgacgatg tctttgccca tggagcggat cagttcgatg gtgccggcgt cgaccatgga
     1621 gacatacatg atggagttgc ggggagaata ctgcatggcg atgcgattcc acggcgcgag
     1681 catggcgtcg aggcgggttt cgagttcctg ccacgaggaa taaacgcctt tcgagccagg
     1741 aagcgagtcc agtttgtcgg cttcgatgcg atggacaagc ttgcgaggtt cgccttcagc
     1801 cgggatgacg tagtaccagc ggcggctgac gagtccgtga tcggggaggc cgaggatggc
     1861 ggcggcgagc gggtcgcggt ggtggtgatc gtagaagagc caagcgtcga gacgtgcgcc
     1921 tcgcagcgct gcctggattg catcgaggtc catgagcgta gggtacaggg tgcacggttc
     1981 agggtgtaga aaaacagaga cgccctgcgg ttttagcggc gtatctttcg aatgggtgtc
     2041 gtccactgcg gtcactatga agcggtttct gaatattctg aaattcaccg ttaaggttgt
     2101 gctggttact gcggtggcgg tgtgggtcgg ggactgggcg ctttttcgcg tgcgtgcagc
     2161 caggggcaca gcattcgaca cggtgcaggt ggaggaatac ctgaccactg ccctgaaggg
     2221 caacaagcag gagtacgact atatggggac cgcgcaggtg acatgcgcgc gagcggtgtt
     2281 tccgcatggc ggagcgccgg catgctggtg ggtgcgccgg cacaggacag agtgggaatg
     2341 agggcagcgg aaacgacgcc tgg
//
LOCUS       DASZYN010000378         3480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1109454, whole genome shotgun sequence.
ACCESSION   DASZYN010000378 DASZYN010000000
VERSION     DASZYN010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109454"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VHE33_09595"
     CDS             <1..530
                     /locus_tag="VHE33_09595"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HVT97750.1"
                     /translation="PVLVMVSGPAHGQSLDDARATVMDGLGAILPDAEKAGITIGIEP
                     LHPMYAAERSVVVTLKQANDLAEQLNSPAAGVVVDVFHVWWDPEVHAEIKRARGKIAG
                     FHVSDWPVPLPGILMGRAMMGDGVIEVRRLREAVDATGYDGPIEVEIFNDAVWGMAEG
                     EVMDLIQQRFLEYVV"
     gene            600..2888
                     /locus_tag="VHE33_09600"
     CDS             600..2888
                     /locus_tag="VHE33_09600"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HVT97751.1"
                     /translation="MRSVLGGIRQGSGVLLGMAASLTLAWFAMTAIAAPMQEPHSEGA
                     AIHYDSATRVFRLDAAGVTYAFGVNEVGQLQSLYWGARLDAGDAFPAAHSQPERASFD
                     PSVDTTPQEFVGWGGALYVEPDVKITFPNGNRDLVLHYVSHRIEGNRLLVTMKDISLD
                     VLVVLEYEIDPGTGILRRSATIENQTEAPFTIEQAFAATWNLPRSNEDRLRYLSGRWA
                     GEWNVQEQLVRPGKTVLESRRGTTGAQNSPWFAIDHDGENDPEHGGVWFGALGWSGSW
                     EITVEQNQVRDIRVSGGFNSFDFGYRLEKGQSLRTPDFYAGYTENGIGGASRLLHQFE
                     VASILPHAPDPALRPVLYNSWEATEFKVDEAGQMALAEKAASIGVERFVMDDGWFGER
                     NDDHAGLGDWYPNPQKFPHGLKPLIDKVHGLGMSFGLWVEPEMVNPNSQLYREHPDWV
                     LNFPDRPRNEGRHQLVLNLARPDVRKWMFTTLDNLLNQNAIDFLKWDYNRNWSEPGWP
                     QVAPDQEKNVYVDFIRNYYSILAELREKHPKVEIESCSGGGGRVDLGVMHYTDEVWPS
                     DNTDAFDRLTIQNGFTYAYTPAVMMAWVTDSPTWVNHRTLSLEYRFLSSMQGSLGVGA
                     NLNHWTPEDFATAKKMIEQYKSVRETVQRGRLYRLIEPIGGSEQSATEYVGRDGHQAV
                     VFAFLHSSGEMYPYPRLYLRGLDRNAVYRVSAMDGKLAAETPQEASGAWWMDHGVDVH
                     LIGDFQAAAFTLERVSAPMRTP"
     gene            2972..>3480
                     /locus_tag="VHE33_09605"
     CDS             2972..>3480
                     /locus_tag="VHE33_09605"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HVT97752.1"
                     /translation="MPSRLSGAMRLVAQPRKLRFLPLLAATYFMVSGGPYDIEDIIGY
                     GGYGRALVLLFVLPFFWSFPTALMLGELASAVPAEGGFYAWVHRALGPFWGFQEAWLS
                     LASSVFDMAIYPTTFVLYLRQLAPDLTAGHRGLLLELSVVGVALVWNLRGARAVGEGS
                     IGLWAVSISP"
BASE COUNT          629 a          881 c         1243 g          727 t
ORIGIN      
        1 cgccggtgct cgtcatggtt tccggtccgg cacacggcca gtcgctcgac gatgcgcgag
       61 cgacggtcat ggatggattg ggtgcgattc ttccggatgc ggagaaggcg ggcatcacga
      121 ttggaattga gccgcttcat cccatgtacg cggcggagcg gtctgtggtc gtgacgctga
      181 agcaggcgaa cgatcttgcg gagcagctca acagccctgc cgcgggcgtg gttgtcgatg
      241 tattccacgt gtggtgggat ccggaagttc atgccgaaat caagcgggca cgaggaaaga
      301 tcgcaggatt tcatgtgtcg gactggccgg tgccgcttcc cggaattctg atgggccgcg
      361 cgatgatggg cgatggggtg atcgaagtgc gcaggctgcg cgaggcggtt gacgccaccg
      421 gctatgacgg tccgattgag gttgagatct ttaatgatgc ggtatggggc atggcggaag
      481 gcgaagtgat ggatctgatt cagcagcgtt ttcttgaata tgtggtttga gcagcgtcat
      541 cgattatccg gtgaagcgat ccttggcgca gaaatgtaac cggtatgagg aggagacgga
      601 tgcggtcagt gctgggaggg atcaggcagg gatcgggcgt gcttttgggg atggcagcgt
      661 cgctgacgct tgcctggttt gcgatgacgg cgatcgcggc gccgatgcag gagcctcact
      721 ccgaaggcgc ggccatccat tacgacagcg cgacgcgggt ctttcgtctt gacgcggccg
      781 gcgtgacgta cgcgtttggg gtgaacgagg tggggcagct gcagagcctt tactggggcg
      841 caaggctcga tgccggggat gcatttccgg cggctcattc gcaaccggag cgggcttcct
      901 tcgatccttc ggtggacacc acgccgcagg agttcgtggg gtggggcggc gctctttacg
      961 tggaaccgga cgtgaagatc acgtttccga atggcaatcg cgacctggtg ctgcactatg
     1021 tctcgcaccg catcgagggg aatcggctgc tggtgacgat gaaggatatt tctctcgatg
     1081 ttcttgtcgt cctggaatac gagatcgatc cgggcacggg cattttgcga cgctcggcga
     1141 cgattgagaa ccagacggag gcgcccttca ccatcgagca ggcattcgcc gcgacctgga
     1201 atctgccgcg cagcaacgag gatcggttgc ggtatctgag cgggcgctgg gcgggagagt
     1261 ggaatgtgca ggagcagctg gtgcggcctg gaaagacggt gctcgagagc cggcggggaa
     1321 cgacgggtgc gcagaacagt ccctggtttg cgatcgatca tgacggcgaa aacgatccgg
     1381 agcacggcgg tgtgtggttc ggagcgctgg gctggagcgg atcgtgggag atcacggtcg
     1441 agcagaacca ggtcagggat atacgtgtga gcggaggctt caacagcttt gattttggct
     1501 atcggctgga aaaggggcag agcctgcgca cgccggactt ctacgccggc tatacggaga
     1561 acgggatcgg tggcgcatca cgactgctgc atcagttcga agtggcctcg atcctgccgc
     1621 acgcgccgga tccggcgcta cggcctgtgc tgtacaactc gtgggaggca accgagttca
     1681 aggtcgacga ggctggccag atggcactgg cagagaaagc cgcgtcgatc ggtgtcgagc
     1741 gcttcgtgat ggacgatggg tggttcggcg agcggaatga cgaccatgca gggctcggcg
     1801 actggtatcc gaatccgcag aagtttccgc acgggctgaa gccgctgatc gacaaagtgc
     1861 atgggctggg gatgagcttt ggattgtggg ttgaaccgga gatggtcaac ccaaacagcc
     1921 agctttatcg tgagcatcct gactgggtac tgaattttcc tgatcgtccg cgcaatgaag
     1981 ggcgccatca gctggttctg aacctggctc ggcccgacgt gcgcaagtgg atgtttacga
     2041 cgctggacaa tctgctgaac cagaacgcga ttgattttct gaagtgggat tacaaccgca
     2101 actggtcgga gccgggctgg ccgcaggttg cgccggacca ggaaaagaac gtttacgttg
     2161 atttcattcg caactactac agcattctcg ccgagctgcg cgagaagcat ccgaaggtgg
     2221 agatcgagag ctgctcgggc gggggcggcc gcgtcgatct gggcgtgatg cactacacgg
     2281 atgaggtgtg gccgtcggac aacactgacg ctttcgaccg gctgacgatc cagaacggct
     2341 ttacctatgc ctacacgccg gcggtgatga tggcgtgggt cacggattcg ccgacgtggg
     2401 ttaaccatcg cacgctgtcg ctggagtatc gattcctctc ctcgatgcag ggctcgctgg
     2461 gtgtgggggc gaatctgaac cactggacgc cggaggattt tgcgacggcg aagaaaatga
     2521 ttgagcagta taagtcggtt cgcgagacgg ttcagcgcgg aaggctctac cggctcatcg
     2581 agcccatcgg cggaagcgaa cagtcggcga ccgaatatgt gggacgggat ggtcaccagg
     2641 cagtggtgtt tgcattcctg cattcgagcg gcgaaatgta tccttacccg cggctctatc
     2701 tgcgagggct ggaccggaat gccgtttatc gggtgagtgc catggacggg aagctggccg
     2761 cggagacgcc ccaggaggca agcggcgcat ggtggatgga tcatggagtg gatgtgcatc
     2821 tgattggaga tttccaggca gcggcattca ctctggaacg cgtgtctgct ccgatgcgga
     2881 ctccctgacg cggttgagcc actgttgcgg cagcgggtac gagggacctt gccggagctg
     2941 cgggggcaga tcgtttctgt agagtaattg catgcccagc cggctctccg gagcgatgcg
     3001 cctggtcgcg cagccgcgga aactgcgttt cctgccattg cttgccgcga cgtatttcat
     3061 ggtctcgggc gggccgtacg atatcgagga catcatcggt tatggcggct atggccgcgc
     3121 tctggtgctg ctgttcgtgt tgcccttctt ctggagtttt cctactgccc tgatgctggg
     3181 ggaactggcg tcggcggtac cggcagaggg tggtttctac gcgtgggttc accgggcgct
     3241 tggtccgttc tggggctttc aggaggcgtg gctctcgctg gcatcgtcgg tgttcgacat
     3301 ggcgatctat cccacgacct ttgttcttta cctgaggcaa ctggctccgg acctgacggc
     3361 agggcatcgc ggactgctgc tggaactgtc ggtggtgggg gtggcgctgg tgtggaatct
     3421 gcgcggcgcg cgcgcggtgg gcgagggctc gattggattg tgggcggttt cgatttcacc
//
LOCUS       DASZYN010000379        18274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_335261, whole genome shotgun sequence.
ACCESSION   DASZYN010000379 DASZYN010000000
VERSION     DASZYN010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 18274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18274
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335261"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VHE33_09610"
     CDS             complement(<1..700)
                     /locus_tag="VHE33_09610"
                     /inference="COORDINATES: protein motif:HMM:NF014206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HVT97753.1"
                     /translation="MQTTTKYSMALSKLAIGFCGLILTATAFADNSTNNHRVLVRDIT
                     SVQGVRDNMLVGYGLVVGLTQTGDSQQTYFTVQTLANAMQKMGVLIPPGTVQVRNVAS
                     VFITASLPPFARPGEKIDVTASSVGDAKSLAGGVLLMTALHGPDGQIYAEAQGPLLIG
                     GYSVGNAQNGKQVNTPTVGMVPNGGIVERDTAVDLHDFKTVSLLLHNPDFTTARQIAD
                     AVNDEFHKPVASALD"
     gene            complement(700..1392)
                     /locus_tag="VHE33_09615"
     CDS             complement(700..1392)
                     /locus_tag="VHE33_09615"
                     /inference="COORDINATES: protein motif:HMM:NF014195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HVT97754.1"
                     /translation="MRQLLLFVTMAAITAGSAQAGKTKAASPAQLRSQYIARLEEHDS
                     VPSPHTTGSLWSPGGAFDELSVDYKARKLNDTVVILVSVQTTAAQNKDVNSSRAFQTA
                     SGITGLAGAINTKGLNPLFNANSATTLKGSGATDSSTTFQTSMTGQVIAVLPSGNLVL
                     EAQRKIFLNNQHEDVTIRGVVRPNDIGPNNTVTSSALSNLEIEMKGKGILADSTRPLN
                     PVTRAILWLVGF"
     gene            complement(1410..1967)
                     /locus_tag="VHE33_09620"
     CDS             complement(1410..1967)
                     /locus_tag="VHE33_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella basal body P-ring formation protein
                     FlgA"
                     /protein_id="HVT97755.1"
                     /translation="MTRLKSFLPWILLFAPLPALAASGRYAISSENVAGAMATMGMQV
                     SPTQLTLLSDPVATTTNPQLRVQSMQRWPGDKIVARLECATTQQCLPFFVSVRLGNMA
                     TSQIAAASDSPAPEPAIRSTPAMVVHAGSPATLYIEGDHIHIRVAVRCLQSGAIGQTI
                     RAEGPDRQQTYTAQVAGAGVLKGRL"
     gene            complement(1978..2769)
                     /gene="flgG"
                     /locus_tag="VHE33_09625"
     CDS             complement(1978..2769)
                     /gene="flgG"
                     /locus_tag="VHE33_09625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HVT97756.1"
                     /translation="MFHALYTAASGMTAQQLNLDNIANNLANSSTAGFQERRVQFSDL
                     LYQNDVMPGAAATQQTTVASGLQIGLGTRPGNTEIVQTQGDLTNTGNPLDLAIEGSGF
                     FQILQPNGQLGYTRAGSFQPDAQGNLVTANGDQLQPSISVPQSATNITIGRDGTVSAT
                     LPGQTQAQQVGIIQLAMFANPGGLNSIGNNIYMPTTASGDPVVGAPGGSQGLGTIQQG
                     MLEQSNVNVVDEFVNMILAQRSYESNSRVIKAADEMLQQLNQLAQ"
     gene            complement(2784..3521)
                     /gene="flgF"
                     /locus_tag="VHE33_09630"
     CDS             complement(2784..3521)
                     /gene="flgF"
                     /locus_tag="VHE33_09630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HVT97757.1"
                     /translation="MDSGYYAACTALVSRTSELDTIANNLANASTAGFRASENVFSTV
                     LADAAGPTGSALVDAMNQYGLMSGTTLDLSQGTLQKTGNTLDVAIEGKGYFTVQTANG
                     TAYTRNGAFQISSKGQLVTSTGDPVLGENGPIPMPAGKVAISADGTISLDGAVSGKLK
                     LVEFKPGTQITSLGNSYYSVPANAVQPPKESAVRQGFLENSNVNPVAGMVELVEAQQS
                     AEIMQRALSMFNSEMDKTATQDLPKAG"
     gene            complement(3719..4435)
                     /locus_tag="VHE33_09635"
     CDS             complement(3719..4435)
                     /locus_tag="VHE33_09635"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HVT97758.1"
                     /translation="MSNAMVFGFPAGRSMAQVPQFPTESLDDLPDVPALPETLLLMEL
                     RLHDFSVDLGELSQLVLSDLGATLQILRLAARECAGDRPARIEDCISHLGPEACLRVA
                     GRRTVLSEARHRGVIEVWAHAREVAQLCRKIAVAGGDRDHPDEAYMVGLMHSLGALPE
                     VLGWPWPQALVNGWTTKGKRLAETWSLPSCVQLLFAEPQTPGTEKGWNSLLRKAHQMA
                     RISPRCPMCAGMAPQLCSRD"
     gene            complement(4486..5376)
                     /locus_tag="VHE33_09640"
     CDS             complement(4486..5376)
                     /locus_tag="VHE33_09640"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97759.1"
                     /translation="MKSLLPPINLGWNHVRARCASPSCHNKLLMRPVPTKSAGIRIGQ
                     DWYCGVDCFVAGARFPLAALSARRLMEMPRNPRLSLGLALLSKDLLTEDQLRLATEVS
                     QKNGVDLEETLLVLGLTNEKHLAAARSVQWGYPVLNQERAGRMVEVEIPLTLLRSFSA
                     APLFYSAGTKRLVLGFVERVDHHLLQSIEQVTGYRAEPCFLTAAEFSEQMQRVVLPVF
                     YEEVVLEEPGDPGQMARALGRYALDAAATDAAFVQCKSWVWARLTGKRRIVDVLFARA
                     NASRTPKNPGTKFAPRIVGA"
     gene            complement(5407..6201)
                     /gene="fliN"
                     /locus_tag="VHE33_09645"
     CDS             complement(5407..6201)
                     /gene="fliN"
                     /locus_tag="VHE33_09645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HVT97760.1"
                     /translation="MGENANNKKGARVFAAAFATAVADALGRTVGAPWPLEVMEGAEP
                     SVRQGQPVHFRLSVDGALRGECFVEFYEPQLNALAAKILGRPPVDDEGENALVKILAG
                     ATAGLKILLTPQFGELTFRLDRVSGLAFGGMFVVQLAAPADESGAPVLLYFDGQLVAA
                     LCAVSSGVGTSTVGAPRFESGNLQLVMDVELNVSLRFGQRQLPLREVLELSSGSVIEL
                     DRMVDEPVELLLDGKVIARGEAVIVDGNYGLRVTEIPQPVASHLMN"
     gene            complement(6204..7157)
                     /locus_tag="VHE33_09650"
     CDS             complement(6204..7157)
                     /locus_tag="VHE33_09650"
                     /inference="COORDINATES: protein motif:HMM:NF014235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HVT97761.1"
                     /translation="MPDQALKEPSTATGTDGPRAKEKNIVTCNFRAAGRLSNENARAL
                     NTVHEAFARRLAIALDSWLGTGVEVKPKGVEQQPIKDHIAGIAPLTYIVPLALSSIQS
                     SMIVECDANIVFAIIEVLLGGTGALGGASRELSEIEEEIMQDVVALVARQVEGVWHFP
                     NLALAPGRRIKPSLVQQYGQANERLAIAKFEMELGPAKGTFQMALPAVFLGALIQQIK
                     QDEPQKKGSVRYFPRPNIRERILDCDIEVAAELPGLRVAVRDLVALQPGSVLKLRAPV
                     RQPGMLSAGGQGIFEAVPVRNGTQKAAQLGRRVTTTNWERE"
     gene            complement(7150..7941)
                     /locus_tag="VHE33_09655"
     CDS             complement(7150..7941)
                     /locus_tag="VHE33_09655"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HVT97762.1"
                     /translation="MTAAELAYSRSAAVGENADDLVLQELPQVYYIAQRIRERLPQQV
                     ELEDLVSAGVIGLLEANRSFDPTRNAQFKTFAKFRIRGAILDSLREIDSGSRLLRRRG
                     REIAETTARLESKLGRHPSEAEIAEEMQIDLEHLRRITAQIDSLHIGAQQVTATDGGS
                     ETIDVIESAPDLDDPNPFDLCLEGEMKSHLAEAVARLTEREQLILSLYYREELTLKEI
                     AQVVGVALSRVSQIRQAAVIKLREYLAHLEQKPSFSTTRGAYHHA"
     gene            complement(7979..10033)
                     /gene="flhA"
                     /locus_tag="VHE33_09660"
     CDS             complement(7979..10033)
                     /gene="flhA"
                     /locus_tag="VHE33_09660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HVT97763.1"
                     /translation="MSTLHTYSDWVIPVGAVALVFVMLVPLPSFALDLLLTLNITAAV
                     LVLLTAIQVLRPAQFSVFPSLILLLTLMRLALDVASTRRILLHGSEGSSAAGKVIEAF
                     GQFVVGGNYIVGFVLFLALIAIQYLVVSHGAVRTAEVTARFTLDAMPGKQMAIDSDLN
                     TGLINAEQARQRREQVAREAEFCGAMDGAARFSQRDSMATILITAINIIAGFLIGVFQ
                     QGVPFAEALKTYTILTVGDGLVAIIPSLLVSVAGAIVVTRAASDHSLGVDIGKQVFKT
                     SRPLWIAGGALLALAVIPGMPKFSFIALAGITMFAAWKMKPPAPDPNAKAAAAPKPGT
                     PGAPVIDPMDAVLKLDELMLEVGVGLVPLVDAKQGGQLLSRVKSLRKTLAQQLGFLVP
                     SIHITDNLSLREREYVVHLRGVEIARWELRRDRLLAVNSNPKPGDLPGQETREPAFDS
                     PARWITPDHQAQAIAAGYAVVDHTSALSAHLAELIRQNAHELLTRSETKRLIDRLADS
                     HPKLIEELTPKLMSLGEIQKVLQQLLREQVSIRDLGTILETMVETAAVNKNPVILVEA
                     CRHALGRALIRPLLDDRGQLKVVTLDSSIEEECMRTGMQNQAASSGSAIQVSVARRVL
                     DGLRAMLGDQFAVAPPVLLCASPGRFYLRRHLEPFIPRVVVISPSEIPPMTQVMSLGS
                     VR"
     gene            complement(10051..11160)
                     /locus_tag="VHE33_09665"
     CDS             complement(10051..11160)
                     /locus_tag="VHE33_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HVT97764.1"
                     /translation="MADANKTEKPTPRHRKKARDRGQVTRSRELSGALAMAAAAGVLW
                     YMGQGAVYQWTAYFRNVLSLASSDSIDPNGPLLFWTSVEAMRWIFPLLLAALAVALFS
                     GFAQGGFVFAPEALSPKAERINPGTKLQQMVSLAALSTILKSLLPFGAIAWVGYACIR
                     SHWTVILLSSYTDARQFSGMISGILLEIAWKSGLILLGWGVVDYALLWRKNESDLKMS
                     REDLREEMKQSDGNPQSKARIRKLQRHNRRKQMLKAAETATVVVTNPTHYAVALRYEV
                     NMPAPIVVAKGLDNLARRIKEIAWERDIPVMENKPLAQALYKGAEVGDPIPAALYHAV
                     AEILVLVYKAQAEVRKREAQRRAASMNPIPQARPL"
     gene            complement(11153..11938)
                     /gene="fliR"
                     /locus_tag="VHE33_09670"
     CDS             complement(11153..11938)
                     /gene="fliR"
                     /locus_tag="VHE33_09670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HVT97765.1"
                     /translation="MIEIPLLTIIAGALTIGVRLSGLMLFAPFFGSMAIPARVKAILV
                     LALTILLYPAVGHSLAARSLMEWPLLIVTEFTIGAGMGIATNLIFEAAQMSGQVIGIQ
                     MGYSLVNILDPQTQVDTPVMSMFYQSIVMLLFLRMDVHYWLLRAVGNSFDYLPPGSGH
                     VTGLFTMGILKTVGDIFGLGIQIAAPVLSATLAADVVLGLLGKASPQMPLMLMGPAIK
                     SLLGIVILIATLKYWPDLFRRLFLDAMTNGDRILHFAATGLHG"
     gene            complement(11935..12204)
                     /locus_tag="VHE33_09675"
     CDS             complement(11935..12204)
                     /locus_tag="VHE33_09675"
                     /inference="COORDINATES: protein motif:HMM:NF013480.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HVT97766.1"
                     /translation="MNVDQVVYLGRLMLTEVVLLAGPVLAVAILVSLVVNVAQVLTSL
                     QDMTISTVMRLVATGGALFIAMPWMWRHLAHFTISLLSDFHTYLQ"
     gene            complement(12204..13181)
                     /gene="fliP"
                     /locus_tag="VHE33_09680"
     CDS             complement(12204..13181)
                     /gene="fliP"
                     /locus_tag="VHE33_09680"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HVT97767.1"
                     /translation="MTLGSCLSRCAIGASLILGLAAPVAAAQASAAAGTNTNATPAAS
                     SSAPANTAQTGTGNAGNAPNAANPASGKAEQTKSAAENKPSRPPVRPSGVRNQLNPAS
                     LPPVPGGGIQLQGVPGGSVPWSIVILLTGLTLIPSLLLCVTPFARLLIVFHFLRQALG
                     LQTTPSNQTLIGLSLILTFFLMQPVGTQIYENAVVPMQAGSITATDALQRGEVPMRKF
                     MAHYVREKDVALFVELAKEPRPASMDDVSMRVLLPAYIVSELKAGFQIGTVLFLPFLI
                     VDMVVASITTSVGMLQLPPVVISTPLKLLLFLMVDGWHLLIGSLMRSFN"
     gene            complement(13178..14383)
                     /locus_tag="VHE33_09685"
     CDS             complement(13178..14383)
                     /locus_tag="VHE33_09685"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HVT97768.1"
                     /translation="MTTAPLRVPHAPSKAAPAREPIQFPTAATSERGPLQTTRRPVRD
                     SSFLSALIDCTKEQSQKEGAAQTQLCADPDEQRRSDDEAKIRRALAEERTRKDSERQN
                     RLEALAQQRHDAEEQQRRQAEEQARLLQEQTLRAAEDQARLEAEEEARWAALQQQRRD
                     AEAQALRAAEEQRLRDEEEQALRETEELQRRFQQELDRLAVELRRREEAEAARIDAEE
                     QKRREAQENFRLAAEERARLEAEEVRAQEEVKAEREAEERFRAAMERVRQAAEERRLR
                     EAQENAENPEPAVAAEEEQPPALNLTEEIELDYTPAATVLFKQSAPTAPGFLKRAWSW
                     MNKKYVPAKQLRVAETISLGEKRFVAVVQVEGRKFLIGGGASGVALLTQLGDTAVPAS
                     GMNPNGGLG"
     gene            complement(14380..14682)
                     /locus_tag="VHE33_09690"
     CDS             complement(14380..14682)
                     /locus_tag="VHE33_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor C-terminal
                     domain-containing protein"
                     /protein_id="HVT97769.1"
                     /translation="MSAATVNPEPKTAETTISARVQNSDELLDSMAWLPCRVTVELPV
                     VRFTIGDLLALTRGSVVETACNQTSDVPLRVNRLLIGWTEFEVIGDRLAVRITDQA"
     gene            complement(14993..16234)
                     /locus_tag="VHE33_09695"
     CDS             complement(14993..16234)
                     /locus_tag="VHE33_09695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HVT97770.1"
                     /translation="MSAFSIPLSGLAASSDALNVIANNLSNLNTDGYKEETLNFADVF
                     NQLQGVSGNGDPIQIGSGVHVAGQTANYTNGTVNSTGISSNLALQGNGFFVVENGSGQ
                     MSYTRDGAFSVNSQGELCTAQGQLVLGYPAVNGVVSTSVALAPINVNQAANIPAVQTS
                     TFSMDTNLNAGSAPGDSYSTPLTVYDSLGTQQTLTVNFTNTAPGAWNYSITLPAAATG
                     GTGSPTTLASGSMTFDSSGNLTSPASPITGIQVSGLADGAAPMSMNWTLTGPGGVSYI
                     TQQTGTSATTSTAQNGYGVGTLTGYSVLSDGTVQGQFSNNQTMALGRIAVASFANNQG
                     LSQLGNNDLQATFASGSPVIGQAGAGGNGTITGGAVEESNVNLSAEFANMIVAQQSYD
                     ANAKVLTTMNQVSQATIQMVS"
     gene            complement(16234..16737)
                     /locus_tag="VHE33_09700"
     CDS             complement(16234..16737)
                     /locus_tag="VHE33_09700"
                     /inference="COORDINATES: protein motif:HMM:NF015896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HVT97771.1"
                     /translation="MNIQGFTPMINAPMQPQAASTNGTSGSNNSTDTGNSSLSAGNLQ
                     GTFINLLVTELQNQDPTQPVDPSQMVAQMVSLNQLDQLIMMNETLNTIAGTAGTGTSG
                     TGEQGSTASNPAAHAAAAKTSPVAANAFLTQPAGITNPVSGGLMNLYGNIATATTNSN
                     HATQGGR"
     gene            complement(16765..>18274)
                     /locus_tag="VHE33_09705"
     CDS             complement(16765..>18274)
                     /locus_tag="VHE33_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97772.1"
                     /translation="PVTTSSADLRQGQFTGNFRPSIPTEQESASVSSIPQLTIAPAMN
                     TVGAAETSVRNSTAEMTLYPVANPETSRAAKTEAPSQAIPLILQADPNSTAPISTTAA
                     AGPSRTEDPYLTTTILQHSASSRGNSPQQNSDSSDNAANNSVSAAPAISVNTLRSTQQ
                     HDLPFVFAQGLTQTLNPATLASVSTAGSQGHSPATAANDPDVTNQAKASSSPQNAMLA
                     SFHAPSIPIGHVISDLGFSSSTSFAAGAIGKPAGTRADFSGTGKDLGTSSGSGSTRTN
                     SAQTTAASADPAIPGTHSNAAQHAQADASQVNAVAMKTADSSAPQTATLHLAVQQQPL
                     SGTASGATDRLPAEIASSTLPSAAEPTESGATSGINSAKLIQTLSETQMHVGMRSAEF
                     GDISIRTAVSQQQMVAQITVDHSDLGKAIAQHLPAAQTKLGDDLGVRAAIQVTQNGMS
                     FSHQHDSTSHQEQRSFVRQIESTGAAASAETESPTARYAIAAEESDRLDIRA"
BASE COUNT         3424 a         5289 c         5903 g         3658 t
ORIGIN      
        1 tatcgagtgc ggaggctacc ggcttgtgga actcgtcgtt gacggcatcg gcaatctggc
       61 gggctgtggt gaagtcgggg ttgtgcagga gcagcgagac ggttttgaag tcgtggagat
      121 cgacagccgt gtcgcgctcg acgatgccgc cattgggcac catgccgacg gtgggagtat
      181 tcacctgctt gccgttttgc gcgttgccga ccgagtatcc accgatcaac aacggtccct
      241 gggcttcggc gtagatctgg ccgtcgggac cgtggagggc ggtcatcagc aggacgccgc
      301 cggcgagact cttggcatcg ccgaccgagg aggcggtgac gtcgatcttt tcgccgggcc
      361 gcgcgaaggg cggcagcgaa gcggtgatga agacagaggc gacgttgcgc acctggacgg
      421 tgccgggggg aatgagcaca cccatcttct gcatcgcgtt ggcaagcgtc tggacggtga
      481 agtaggtctg ctggctgtcg ccggtctggg tgagaccgac cacgaggcca tagccgacga
      541 gcatgttgtc gcgcacaccc tgcacgctgg tgatgtcgcg aacgagtacg cgatggttgt
      601 tcgtgctgtt atcggcgaat gcggtcgcgg tgaggataag gccgcagaat ccgatcgcca
      661 gcttgctgag tgccatgctg tatttagtcg ttgtctgcat cagaaaccca ccagccagag
      721 aatggctctc gtgacgggat tcagcggacg cgtgctgtcg gccaggattc ccttgccctt
      781 catttcgatt tcgagattgc tcagcgccga ggaggtgacg gtattgttcg ggccaatgtc
      841 gttggggcga accacgccgc gaatcgtcac gtcctcatgc tggttgttga ggaagatctt
      901 ccgctgcgct tccagcacga ggttgccgct gggcagcacc gcgatcacct gaccggtcat
      961 gctggtctga aaggtggtac tggagtcggt ggcgcctgat cctttgagcg tggtcgccga
     1021 gttggcgttg aagagcgggt tcagaccttt tgtattgatg gcacccgcca ggccggtgat
     1081 cccggatgcg gtttggaacg cacgggaact gttcacgtcc ttgttctgag cggccgtggt
     1141 ctgcacagag acgaggatga cgacggtgtc gttgagcttg cgcgccttgt agtcaaccga
     1201 aagctcatcg aatgctccgc ccggcgacca caggctgccg gttgtgtgcg gagacgggac
     1261 gctgtcatgc tcctcgaggc gagcaatata ttgcgaacgc agctgggcgg gagaagcggc
     1321 ctttgttttg ccggcctgcg ctgagcccgc tgtaatcgca gccattgtga caaacagcaa
     1381 gagctggcgc atattgcgaa tcctcttcat cacaaccggc ctttcaggac gccggcgccc
     1441 gccacctggg ctgtataggt ttgctggcga tcggggcctt cggcgcggat ggtttgtccg
     1501 attgcgccac tctgaagaca gcgaaccgcc acgcggatgt ggatgtgatc gccttcgatg
     1561 tagagcgtcg cgggagatcc ggcgtggacg accatcgctg gagtgctgcg aatggcaggt
     1621 tcaggcgcgg gtgagtcgct ggccgcggcg atctgactcg tggccatgtt gccaaggcgc
     1681 acgctgacga agaatggcag gcactgctgt gtggtcgcgc actcaagacg ggcaacaatt
     1741 ttgtcgcccg gccagcgttg catcgactgc acccgcaatt gagggttggt ggtggtggca
     1801 accgggtcgc tcagcagagt gagctgcgtt ggagaaacct gcatacccat ggtggccata
     1861 gccccggcga cgttctccga gctgatcgcg tagcggcccg atgcggcaag cgcaggcagc
     1921 ggagcgaaga ggagaatcca gggcagaaaa gacttcaatc gagtcacaga accctcacta
     1981 ctgcgccagc tgattcagtt gctgcagcat ctcgtcggcg gccttgatga cgcgcgagtt
     2041 cgactcatag gagcgctgcg cgaggatcat gttcacgaac tcgtcgacga cgttcacgtt
     2101 cgactgctcg agcattccct gctggatcgt gccgagcccc tgcgagccgc ctggagcacc
     2161 gacaacggga tcgccagacg cggtcgttgg catgtagatg ttgttgccga tgctgttgag
     2221 gccgcccgga ttggcaaaca tagccagctg aatgatgccc acctgctgag cctgggtctg
     2281 gccgggcagc gtggcgctca ccgtgccgtc gcgaccgatg gtgatgttgg tggccgactg
     2341 cggaacggaa atggagggct gcaactgatc gccgttggcg gtcacaaggt tgccctgcgc
     2401 atccggctgg aacgaaccgg cgcgggtgta gccgagctgg ccgttgggct gaagaatctg
     2461 gaagaagccg gagccttcaa tcgcgagatc gagtggattg ccggtgttgg tgagatcgcc
     2521 ctgggtctgc acgatttcag tgttgccggg acgcgtgccg aggccgatct gcaatcccga
     2581 ggcaacggtg gtttgctgcg ttgccgcggc accgggcatg acgtcgttct gatagagcag
     2641 gtcggagaac tgcacgcggc gctcctgaaa gccggccgtg ctcgagttcg ccagattgtt
     2701 cgcgatgtta tcgaggttga gctgctgggc ggtcatgccg ctggctgctg tgtagagcgc
     2761 atgaaacatc cgtggttctc cttttagcct gccttgggca gatcctgcgt tgcggtctta
     2821 tccatttcgg agttgaacat tgagagagcg cgctgcatga tctccgcgct ttgctgcgct
     2881 tcgaccagct cgaccatgcc ggcgacggga ttgacattgg aattttcgag aaagccctga
     2941 cgcacggccg actctttggg cggctgcaca gcgttcgcgg ggaccgaata atacgaattg
     3001 ccgaggctgg tgatctgtgt gccgggcttg aattcgacga gctttagttt gccgctgacc
     3061 gcgccgtcca gggagatggt gccgtccgcg ctgatggcga ccttgcccgc aggcatgggg
     3121 atggggccat tctcgcccag cacgggatcg ccggtgctgg tgacgagctg gcctttcgat
     3181 gagatctgaa acgcgccatt ccgtgtatag gccgtgccgt tcgccgtctg gacggtgaag
     3241 tagcctttgc cttcgatggc gacatcgaga gtgttgcccg tcttctgcag ggtgccctga
     3301 ctaaggtcga gcgttgttcc gctcatcaag ccatattggt tcatcgcatc gacgagagcc
     3361 gaaccggtag gaccggccgc gtcggcaagc acggtgctga agacattctc cgatgcacga
     3421 aacccagcgg tgctggcgtt cgccaggttg ttggcgatcg tatccagctc cgaggtgcgc
     3481 gaaaccaggg ccgtacacgc cgcgtagtat ccggaatcca tagaacctct ggccagaact
     3541 tatgcacgag gcgtgccaaa acaccaggca aactttgcct ggactcagcc tcgaaacggc
     3601 ttcagaaggg tgtttggatg agggtttgat gagaagtgcc ggggagagag ggtttcccgc
     3661 cagcgcggag gccggcgatc ctcattcgaa ggcaatctgc ccgggctatg aaggaagctc
     3721 aatcgcggct acagagttgg ggcgccatac cggcacacat ggggcaacgc ggggagatgc
     3781 gagccatctg gtgtgctttg cggagcaagc tgttccagcc cttttctgtg ccgggcgttt
     3841 gcggttcggc gaataacagc tgcacgcagg atggcaggga ccacgtttcc gccagccgct
     3901 ttcctttggt cgtccatccg ttcaccagcg cctgcggcca gggccagccg aggacttcgg
     3961 gaagagcacc gagcgaatgc atgagtccga ccatgtaggc ttcatcggga tggtcccggt
     4021 cacccccggc gaccgcaatt ttgcggcata attgcgcaac ttcacgggcg tgcgcccaga
     4081 cttcgatgac accacgatga cgcgcttcgc tcagaactgt cctgcgccct gccacgcgca
     4141 ggcaggcctc gggtccaaga tgcgaaatgc agtcttcgat gcgcgccgga cgatctcccg
     4201 cgcactcgcg cgcggcgaga cgcagaatct ggagagtcgc tccgaggtcg ctcaggacaa
     4261 gctgcgacag ttcacccagg tcaacggaaa agtcatgcag ccgcagttcc atcaggagca
     4321 gagtttccgg gagagccggg acatcgggga ggtcgtccag tgactccgtt gggaactgcg
     4381 gtacctgagc catgctgcgg cccgccggaa atccaaaaac catcgcgttc gacacagatc
     4441 gtctccccgc atagctatcg gcatacgggg acgaggtttc cagtgctagg cgccaacgat
     4501 ccggggcgcg aactttgtgc cagggttctt cggggtgcgg gacgcgttcg cccgggcgaa
     4561 gaggacgtcc acgatacggc gcttgccggt tagcctggcc cacacccacg acttgcactg
     4621 cacaaaggcg gcgtcggtag ccgctgcatc gagagcgtaa cggccgaggg cccgtgccat
     4681 ctggcccgga tcgcccggct cttcgagcac aacttcttca tagaacacag ggagcacaac
     4741 gcgctgcatc tgctcggaga actccgccgc agtgaggaag caaggctccg cacggtagcc
     4801 ggtgacttgt tcaatggact gcaggaggtg atgatcgacg cgctcgacga atccgagcac
     4861 gaggcgtttt gtgcccgcag agtaaaacag cggggcggcc gagaaggaac gcagaagcgt
     4921 cagcggaatt tcaacttcta ccatgcggcc ggctcgttcc tgattgagaa ccggatagcc
     4981 ccactgcacg ctgcgcgccg cggcgaggtg cttttcgttg gtcaaaccca gcacaagcaa
     5041 cgtttcttcg agatcgacgc cgttcttttg tgaaacctcg gtggcgagac gcagctggtc
     5101 ttcggtgaga aggtctttcg acagtaacgc caggcccagg gagaggcggg gattgcgggg
     5161 catctccata agacggcgcg ccgagagcgc ggccagcgga aagcgcgcac cggccacaaa
     5221 gcaatcgaca ccgcagtacc agtcctgccc gatgcggata cctgcactct tcgtgggcac
     5281 ggggcgcatc aacagcttgt tgtggcagct gggcgaggcg caacgcgcac gcacgtgatt
     5341 ccagccaaga ttgatgggag gcagcagaga tttcaaagtc aagttcatcg ggatgggcct
     5401 tgtcgtctaa ttcatgaggt gagaggcgac gggttgcggt atctccgtca cgcgcaggcc
     5461 ataattgccg tcgacaatca ccgcttcgcc gcgggcgatg acttttccat cgagcagaag
     5521 ctcgaccggc tcgtcgacca tgcgatccag ttcaatgacc gaaccgctgg agagctcgag
     5581 gacttcacgg agcgggagct gacgctgtcc aaatcggagc gagacgttca gctccacatc
     5641 catgaccaac tgcagattgc cggactcgaa tcgaggagca cccacagtcg aggtcccgac
     5701 accgctcgat acggcacaaa gtgccgcaac gagttgtccg tcgaagtaaa gcaacaccgg
     5761 agcccccgat tcatccgccg gagcggccag ttgcacgaca aacatgccgc cgaaagctaa
     5821 cccggaaaca cgatcgaggc ggaacgtcag ctctccgaat tgaggggtga gcaggatctt
     5881 caggcctgcg gttgcgccgg cgaggatctt aacgagcgcg ttctcgcctt cgtcgtccac
     5941 gggcggccga ccgagaatct tagcggcaag cgcgttgagc tgcggctcgt aaaattccac
     6001 gaaacactcg ccgcgcaaag caccgtcgac cgagaggcga aaatggacgg gttgcccctg
     6061 ccttaccgag ggctcggcgc cttccatcac ttccagcggc cagggggctc caaccgttct
     6121 gcccagcgcg tctgcaacgg cggtggcgaa cgcggccgcg aagaccctgg ctcccttttt
     6181 gttatttgcg ttttctccca ttgctattcc ctctcccagt tcgttgttgt tacgcggcgg
     6241 ccaagctgtg cggccttttg cgtgccgttg cgcaccggca cggcttcaaa gattccctga
     6301 ccaccggcgc tgagcatgcc gggctgccgc acgggtgccc gcagcttgag aacacttccc
     6361 ggctgcagcg cgacgagatc gcgcacggcg acgcgcaggc ccggcagctc ggcggcgacc
     6421 tcgatgtcgc agtcgagaat ccgctcgcga atattcggcc gcggaaaata ccggacgctt
     6481 ccctttttct gcggctcgtc ctgtttgatc tgctggatca gcgcgcccag gaacactgcg
     6541 ggcagagcca tctggaacgt gcctttcgcg ggcccgagtt ccatttcgaa ttttgcgatg
     6601 gcgagccgtt cgttggcctg gccgtactgc tggaccagcg acggcttgat gcgacgtccc
     6661 ggcgccagcg ccagattcgg aaagtgccag actccttcca cctggcgcgc aaccaatgcg
     6721 acgacgtcct gcatgatctc ttcttcgatt tcagagagct cacggctggc accgcccaac
     6781 gcccctgtgc cgccgagaag aacttcgatg atcgcgaaaa cgatgttcgc atcgcactcc
     6841 acaatcatgc tgctctgaat cgagctgagc gcaagcggca caatatatgt gagcggtgca
     6901 attccggcaa tgtggtcttt gatcggctgt tgttcgacgc ccttcggttt cacctcgaca
     6961 ccggttccga gccagctgtc gagagcaatg gccaggcgac gagcaaaggc ttcgtgaacc
     7021 gtgtttaaag cgcgggcgtt ctcgttcgag aggcgacccg cggccctgaa attgcaggtc
     7081 acaatgttct tctcttttgc gcgagggccg tcggtgcctg tggccgtgct cggctctttc
     7141 agcgcctgat caggcatggt ggtacgctcc tctggtggtc gagaatgatg gcttctgttc
     7201 gagatgcgca agatactcgc gcagcttgat gaccgcggcc tggcgaatct gggagacgcg
     7261 cgacagcgcg acgccgacga cctgggcgat ttctttcagc gtgagttctt cccggtagta
     7321 aagagagagg atcagctgtt ctctttcggt cagcctggcg acagcctcag ccaggtggga
     7381 tttcatttcg ccttcgagac acaggtcgaa tgggtttgga tcatccaggt ccggggcgga
     7441 ttcgataacg tcgatggttt ccgaaccgcc gtcggtggct gtgacctgct gtgcgccgat
     7501 gtgcagagaa tcaatctgtg ccgtgatgcg gcgaaggtgc tccagatcga tctgcatctc
     7561 ctcagcaatt tcggcttcgg acggatggcg gccgagtttt gattccagcc gagcggtcgt
     7621 ctcggcaatc tcacggccgc ggcggcgcag caggcgcgat ccggaatcga tttcgcgcag
     7681 gctgtcgagg atggcaccgc gaatgcggaa ttttgcgaag gtcttgaatt gcgcattgcg
     7741 ggttgggtcg aagctgcggt tcgcctcgag caggccgatc acgcccgcgc tcacgaggtc
     7801 ttccagttcc acttgctggg gaagacgctc gcggatgcgc tgcgcaatgt agtaaacctg
     7861 cgggagctcc tgcaatacca gatcatcggc gttctcgccg accgccgcac tgcggctgta
     7921 ggctagttca gctgccgtca tcgttgcacc tcacctgaca taagacccgt gaaccctttc
     7981 atcgcaccga ccccagcgac atcacctgcg tcatgggcgg aatctccgac ggagaaatga
     8041 ccacgaccct gggaataaat ggttccagat gccgtcgcag atagaagcgg cctggcgatg
     8101 cacagagcag aaccggcgga gccacggcaa actggtcgcc gagcatggcg cgcaaaccat
     8161 cgagcacccg acgcgcgacc gatacctgaa tggcactccc cgacgatgcc gcttgattct
     8221 gcatcccggt tcgcatgcac tcttcctcga tggagctgtc gagcgtgacc accttcagct
     8281 gaccgcgatc gtcgagcagc gggcgaatca gcgcgcggcc cagagcgtgg cggcacgcct
     8341 ccacgagaat gaccggattc ttgttgactg ccgccgtttc gaccatggtt tcgaggatgg
     8401 tgccgaggtc gcggatggaa acctgctcgc gcagcaactg ctgcagtact ttttgaattt
     8461 cgccgaggct catgagcttg ggggtgagct cttcgatcag cttcggatgg gaatccgcga
     8521 ggcgatcgat caggcgtttc gtctccgagc gcgtgagcag ctcgtgggcg ttctgccgga
     8581 tcagctcggc aagatgcgct gacaatgccg acgtgtgatc gacaaccgcg tatccagcgg
     8641 cgatcgcctg ggcctggtgg tcgggcgtga tccaccgggc gggtgaatcg aaggcgggtt
     8701 cgcgtgtttc ctgaccaggg agatcgccgg gctttggatt cgagttgacg gcaagcagcc
     8761 tatcgcggcg cagctcccag cgcgcgattt ccactccgcg gagatggacg acatattcgc
     8821 gttcgcgcag agacagattg tcggtgatgt ggattgaagg aacgaggaat ccgagctgct
     8881 gcgccagggt cttgcgcagc gacttcacgc gcgagagaag ctggccaccc tgctttgcat
     8941 cgaccagcgg gacgaggcct acgccgactt cgagcatcaa ctcgtcaagc ttgaggacag
     9001 catccatcgg atcgatgacc ggcgcgcccg gggttcctgg cttcggcgcg gccgcggcct
     9061 tcgcgttggg atctggagcg ggtggcttca tcttccaggc agcaaacatt gttatgccgg
     9121 ccagcgcgat gaaggagaat ttcggcattc cggggatgac ggcgagagcc aggagggcgc
     9181 cgcccgcgat ccagagggga cgcgaggtct tgaagacctg cttcccaatg tcgacgccga
     9241 gagaatgatc ggaagccgca cgcgtaacga cgatggcgcc ggcgacggaa accagcagcg
     9301 aggggatgat ggcgacgagc ccgtcgccaa ccgtgaggat cgtgtaggtc ttgagagctt
     9361 cagcaaaggg cacgccctgc tggaagacgc cgatcaggaa ccccgcgatg atattgatgg
     9421 cggtgatcag gatggtggcc atcgagtcgc gctggctgaa gcgggccgcg ccgtccatcg
     9481 cgccgcagaa ctcggcttcg cgcgcaacct gctcacggcg ctggcgagcc tgttcggcgt
     9541 tgatcagtcc ggtgttcagg tcggagtcaa tcgccatctg cttgcccggc atggcatcga
     9601 gggtgaagcg ggcggtgact tctgccgtac gaaccgcgcc gtggctgacg acgagatact
     9661 ggatcgcgat gagggcgagg aagaggacaa agccaacgat gtagttgccg ccaacaacga
     9721 actggccgaa ggcctcaata accttaccgg ccgccgacga accttcgctg ccgtggagaa
     9781 gaatgcgccg tgtactggcg acgtcgagcg cgagccgcat gagcgtgagc aggagaatca
     9841 ggctggggaa aaccgaaaac tgggctggac gcagaacctg aattgccgtc agcaatacca
     9901 gcacggcagc ggtgatgttg agcgtgagca acaggtcgag cgcgaagctc ggcagaggca
     9961 cgagcatgac aaagacgagt gctaccgcgc caaccgggat cacccagtcg gagtaggtat
    10021 gcagcgtgga catcaatcct ggcttcttgt tcacagcggc ctcgcctgag gaatgggatt
    10081 catggaagct gcgcggcgct gggcttcccg cttgcgcacc tcagcctgcg ccttgtacac
    10141 gaggaccaga atttcggcca cggcgtgata gagcgcggcg ggaatgggat cgccgacttc
    10201 ggcgcctttg taaagagcct gcgcgagcgg cttgttttcc attaccggaa tgtcgcgctc
    10261 ccatgcgatt tccttgatac ggcgcgccag attgtccaga cccttggcca cgacgatcgg
    10321 cgccggcatg ttcacctcgt accgaagcgc gaccgcatag tgcgtgggat ttgtaacaac
    10381 gacggtggcg gtttcggccg ccttgagcat ctgcttgcgc cggttgtggc gctgcagctt
    10441 gcggatacgc gctttgctct gcggattgcc gtccgattgc ttcatttctt ccctgagatc
    10501 ttcgcggctc atcttcaggt cggactcgtt cttgcgccac agcaaggcgt aatcgacaac
    10561 gccccatccg aggagaatca gcccggactt ccaggcaatt tcgagcaaaa tgccgctgat
    10621 catacccgag aactggcgag cgtccgtgta ggagctgagc aggatcacgg tccagtggct
    10681 gcgaatgcac gcataaccga cccaggcaat ggcgccgaac ggcagcaacg atttcaagat
    10741 cgtgctgagg gcggccagcg agaccatctg ctggagtttt gtgccagggt taatgcgttc
    10801 tgctttcggg gacagcgctt ctggcgcgaa cacgaatcca ccctgggcga acccggagaa
    10861 gagagctaca gccagcgccg ccagcagtag cgggaagatc cagcgcatcg cttcgacgct
    10921 tgtccagaag agcagagggc cgttcggatc gatcgagtcg gagctggcaa gagataagac
    10981 attgcggaaa taggccgtcc actgatacac ggcgccctgt cccatatacc aaagcacgcc
    11041 agccgctgcc gccatggcaa gggcgccgga gagttcccgc gaacgggtga cctggcctcg
    11101 gtcgcgagct ttcttccgat ggcgcggtgt cggtttttct gttttattcg cgtcagccat
    11161 ggagtcctgt tgcagcgaaa tgcagaatgc ggtcgccgtt ggtcatggcg tcgaggaaca
    11221 agcgccggaa gaggtccggc cagtacttga gcgttgcgat gagtatgacg attccgagaa
    11281 ggctcttgat tgcagggccc atcagcatga gaggcatctg cggcgacgct ttgccgagca
    11341 atccgagcac gacgtcggcg gccagggtcg ctgaaaggac cggggcagcg atctggatcc
    11401 ccaggccaaa gatgtcaccc acggtcttca gaattcccat cgtgaagagg ccggtcacgt
    11461 gcccggagcc cgggggaaga tagtcgaagc tgttgcccac ggcgcgcagc aaccagtagt
    11521 ggacgtccat gcggagaaac agcaacatga cgatgctctg gtaaaacatc gacatcaccg
    11581 gcgtatcgac ctgggtctgg gggtcgagga tgttgaccag cgagtagccc atctggatac
    11641 cgatcacctg accgctcatc tgcgccgcct cgaagatgag gttggtggcg atccccatcc
    11701 ctgcgccgat ggtgaactcg gtgacgatga gaagaggcca ttccatgagc gaacgcgcgg
    11761 cgagactgtg gccgacggcg gggtagagca ggatggtcaa tgcaagaacg agaatcgcct
    11821 tcacgcgtgc agggatggcc atgctgccga agaacggagc aaagagcatg agccccgaga
    11881 ggcgaacccc gatggtgagc gcgccggcga tgatcgtaag cagaggaatt tcgatcattg
    11941 gagataggta tggaaatccg agagcagcga aatcgtgaag tgcgccagat gacgccacat
    12001 ccagggcatc gcaatgaaga gagcgccgcc ggtagccacc agacgcatca ctgtggaaat
    12061 ggtcatgtcc tgtagggagg tgagaacctg agcaacgttg acgaccagac tgacaagaat
    12121 cgccacagcg agaacggggc ctgcaagcag aaccacctcg gtgagcatca gccgccccag
    12181 atagaccacc tgatcgacgt tcatcagttg aaactcctca tgagggatcc gatcagcaga
    12241 tgccacccat caaccatgag gaagaggagc aacttgaggg gcgtggaaat cacaaccggc
    12301 ggcaattgca acatgccgac ggaggttgtg atcgaagcga cgaccatgtc gacgatgagg
    12361 aatggaagga acagcacggt gccgatctga aagccggctt tcaactccga aacaatgtag
    12421 gcggggagca ggacgcgcat cgaaacgtcg tccatggacg cgggcctcgg ctccttggcc
    12481 aactcgacaa agagcgccac gtctttttcg cggacataat gcgccatgaa cttacgcatc
    12541 ggcacttcac cgcgctgcaa tgcgtcggtt gccgtgatac tgccggcctg catggggacg
    12601 acggcattct catagatctg ggtgccaacc ggctgcataa gaaaaaacgt gaggatcaga
    12661 gagagaccga tcagcgtctg gttcgaaggg gtcgtttgca gccccagcgc ctggcgcagg
    12721 aagtgaaaga cgatcagcag gcgcgcgaat ggcgtgacac acaacagcaa cgaaggaatc
    12781 agggttagac cggtgagcag gatgacgatg gaccacggca ccgatccgcc gggtacgccc
    12841 tgcaactgaa tgccgccgcc ggggaccggg ggcagactcg cggggttcag ctgattacga
    12901 acaccggaag gacgaacagg tggacgcgag ggtttattct ccgcagcgga ttttgtctgc
    12961 tcggctttgc cggacgcagg gttcgcggcg ttgggcgcat tcccggcgtt gcctgttccg
    13021 gtttgagctg tgttcgccgg tgcactcgat gatgctgccg gagtcgcatt tgtatttgtc
    13081 ccagccgccg cactggcctg cgctgccgcc accggagccg cgagcccgag aatcaggctt
    13141 gcgccgattg cgcagcgcga aagacaactt cccagggtca tcccaggccc ccgttgggat
    13201 tcatgccgga cgccggaacc gctgtgtcgc cgagttgagt gagcagcgcg acgcccgatg
    13261 cgccaccacc gatgaggaac ttacggcctt ccacctgcac tacggcgaca aaacgctttt
    13321 cgcccagcga aatggtctcc gctacccgga gctgttttgc gggcacatat ttcttgttca
    13381 tccaggacca tgcgcgcttt agaaacccgg gtgcggtcgg tgcggactgc ttaaacagaa
    13441 cggtcgctgc aggtgtgtag tccaactcga tctcctctgt caggttcagc gcgggcggct
    13501 gctcttcttc ggcagccacc gcgggttcag ggttctccgc attctcctgg gcttcgcgaa
    13561 gacgcctctc ttctgctgcc tggcgaaccc gctccatcgc tgcgcgaaat cgttcttccg
    13621 cttcgcgctc ggccttcact tcctcctgcg cgcgcacctc ctcagcctcg agccttgcgc
    13681 gctcttccgc agccaggcgg aagttttcct gcgcttcgcg gcgcttttgt tcttccgcgt
    13741 cgatgcgggc cgcctctgct tcctcgcgac ggcgtagttc cactgctaac cggtccagtt
    13801 cctgctggaa ccggcgctgc agttcctctg tttcgcgcag agcctgctct tcttcgtcgc
    13861 ggagtctctg ttcttccgct gcacggaggg cctgggcttc tgcatcgcgg cgctgctgct
    13921 gaagagctgc ccagcgtgct tcctcttccg cttcgagacg cgcctgatct tcggccgcgc
    13981 ggagcgtctg ttcctggagc agccgtgcct gctcttccgc ctgacgccgc tgctgctctt
    14041 cggcgtcgtg gcgctgctgc gccaaagctt caaggcggtt ctgtcgttcg gaatccttgc
    14101 gtgtgcgctc ctcggccagg gcgcggcgga tcttcgcttc gtcgtcactg cggcgctgct
    14161 cgtctggatc agcgcacagc tgcgtctgtg cagcgccctc tttctggctt tgctccttcg
    14221 tgcagtcgat gagcgccgag aggaaagagc tatccctgac cgggcgacgc gtggtctgca
    14281 aggggccgcg ctcagaggtt gccgcagtcg gaaactggat gggctcgcgc gcgggcgcgg
    14341 ccttggaagg ggcgtgggga acgcgaaggg gagctgtggt catgcctgat ccgtgatgcg
    14401 taccgcgagg cggtcgccga taacttcaaa ttctgtccag ccgatcagga ggcgattgac
    14461 ccgcagcggt acgtcactgg tctgattaca ggccgtctcc acgactgaac ctctggtgag
    14521 ggccaggagg tcaccgattg tgaatcggac gacaggcagc tcgacggtca cccggcaggg
    14581 caaccaggcc atgctgtcga gcagttcgtc gctgttttgc acccgtgccg agatggtggt
    14641 ctcagcggtt ttgggctcgg ggttaacggt agcggcactc atcgaattcc ggctggctcc
    14701 tggctccgtc cctgcgcacc acgatccagt gcgcggtgag ggcggagcat tgcaacgagc
    14761 acaaatgcaa tcggattgcc ggaaatacaa acaggtgcgc aatcctgtgt atggcatgag
    14821 cttttcggct catccgcctg cgacacaagg acttgcacac cacatcatgg agcagacagg
    14881 ctctcgccgc gagtgagacg gcgatgagag ccagtctttc tatggaggcc tccgccgact
    14941 ccggggtgga gccggccgag gcctctaatc gttcactaac cgagacgcgg gactacgaaa
    15001 ccatctggat cgtggcctgc gatacctggt tcatggttgt cagcaccttt gcgttggcgt
    15061 cgtagctctg ttgcgcgacg atcatgttgg caaactcggc tgagaggttg acgttcgatt
    15121 cctccactgc gccgcctgtg atggttccgt tgccgccggc acccgcctgg ccgatgaccg
    15181 ggctgcccga agcgaacgtg gcctgcagat cgttgttgcc cagctgggac agaccctgat
    15241 tgttagcaaa gctggcaacg gcaatgcgtc cgagggccat ggtctggttg ttgctgaact
    15301 gtccctgcac ggttccgtca gaaagcacgg aatagccagt caacgttccc acgccatagc
    15361 cgttctgggc ggtggaggtg gttgcactgg tgccggtctg ctgggtgatg taggaaacgc
    15421 caccggggcc tgtcagagtc cagttcatgg acatgggcgc ggcgccgtct gccagtccgg
    15481 agacctggat tccggtgatc gggctggccg gagaggtcag attccccgaa gaatcgaagg
    15541 tcatgctgcc cgaggccagg gtggttggtg agcccgtacc tccggtagcc gcggccggta
    15601 gcgtgatcga ataattccat gcccccggcg ccgtgttggt gaagttcacg gttagggtct
    15661 gctgcgttcc gagcgaatcg taaaccgtca acggcgtgct gtagctgtct ccgggggccg
    15721 agcctgcgtt caggttggtg tccatggaga acgtggaggt ctggactgcg ggaatattcg
    15781 ccgcctgatt cacattgatg ggcgccagag ccacgctggt ggaaactacg ccgttgaccg
    15841 cgggataacc gaggaccaac tggccctgcg ccgtgcacaa ctcaccctgc gagttgaccg
    15901 agaaggcgcc gtcgcgcgtg taactcatct gaccgctgcc gttctcgacc acgaagaaac
    15961 cgttaccctg caacgcaaga ttggacgaga tgcccgtgga attgacggtt ccattggtgt
    16021 aattcgcggt ctgccccgca acatggacgc ccgatccgat ctggatggga tcgccgttgc
    16081 ccgaaacgcc ctgcaactgg ttgaagacgt cggcaaagtt gagggtttct tccttgtagc
    16141 cgtcggtgtt gaggttggaa aggttgttgg cgatgacatt cagcgcatcg gagctggctg
    16201 ccaggcctga aagaggaatc gaaaacgctg acattatctg cctccctgag tggcgtggtt
    16261 ggaattggtg gttgcggttg cgatgtttcc gtagagattc atcaggccgc ccgaaacggg
    16321 gttcgtgatg cccgcaggtt gcgtcagaaa cgcattggcg gcgaccgggc tggtcttcgc
    16381 ggcggctgca tgcgcggccg ggttgctcgc agtggagccc tgctccccgg ttcccgacgt
    16441 accggtgcct gctgtgccgg cgatcgtgtt taaggtctcg ttcatcatga tcagctggtc
    16501 cagttggttg agggaaacca tctgagccac catctggctg gggtcgactg gctgggttgg
    16561 atcctgattc tgcagctcgg tgaccagaag attgatgaat gtgccctgca gattgccggc
    16621 ggagaggctg ctgttcccgg tatctgtact gttgttgctg cccgatgtac cattcgtgct
    16681 cgctgcctga ggctgcatcg gcgcgttgat catcggtgtg aaaccctgaa tgttcattgt
    16741 tccttcctct tcgttacctt tccgtcaggc cctgatgtcc agccgatcgg attcttctgc
    16801 tgcaatggca tagcgcgcgg tcgggctttc tgtttctgct gaagctgccg cgcctgtcga
    16861 ttcaatctgg cgaacaaacg atctctgctc ctggtgggaa gtgctgtcat gctggtgcga
    16921 gaaactcatc ccgttctgcg tcacctgaat cgcggcgcgc acaccgaggt cgtctccgag
    16981 cttggtttgc gccgccggca gatgctgcgc gatggctttt ccgaggtcgc tatgatctac
    17041 agtgatctgc gcaaccatct gctgctgcga aaccgctgtg cggatcgata tgtcgccgaa
    17101 ctctgccgac cgcatcccga cgtgcatctg cgtctcgctc agcgtctgaa tgagcttcgc
    17161 gctgttgatg ccggaggttg ccccggattc tgttggttct gccgctgatg gaagagtcga
    17221 gctggcaatt tcggcgggga gccgatccgt tgctccgctt gcggttccgg agagtggttg
    17281 ctgctgtacc gcaagatgca gcgttgctgt ttgtggagcg gaagaatctg cggtcttcat
    17341 ggccacagcg ttgacttgcg atgcatctgc ctgcgcgtgc tgcgccgcat tgctgtgggt
    17401 gccggggatc gccgggtctg cgctggcggc agtcgtctgc gccgagttgg tcctggtgct
    17461 gccggaccca ctggacgtgc cgagatcctt gccggttccg gagaagtctg ccctggtgcc
    17521 ggctggtttg ccgatcgccc ctgccgcgaa gctcgtcgaa ctgctgaagc cgagatcgga
    17581 gataacgtgg ccaatcggaa tgctgggtgc atgaaacgat gcgagcatcg cgttctgggg
    17641 agagcttgac gcttttgcct gattcgttac gtctgggtcg ttcgcagcgg tcgcgggcga
    17701 atgcccctgg gaaccagcgg tcgaaactga cgccagcgtg gccgggttca gagtctgggt
    17761 caggccctgc gcaaaaacga agggaaggtc atgctgttgg gtgctccgca gcgtgttcac
    17821 tgatattgcc ggcgccgcag acacgctatt gttcgctgcg ttgtcgctgg aatcggagtt
    17881 ctgctgcggg gaattccctc gcgacgaggc actgtgctgc aggatcgttg tcgtcagata
    17941 cggatcttct gtccgtgaag gcccggccgc tgctgtcgtc gatattggtg ccgtggaatt
    18001 agggtcggct tgcaaaatca gaggaatcgc ctgggacgga gcttctgttt tcgccgcacg
    18061 cgacgtttcc gggttggcta ccggatacaa agtcatctcc gcagttgaat tccggactga
    18121 cgtctctgcc gctccaacgg tgttcatcgc cggcgcaatc gtcaattggg gtatggaaga
    18181 gacagaggct gattcctgct cagtgggaat cgatggccgg aaattcccgg tgaattgacc
    18241 ctgccgcaga tccgcactgc tcgtcgttac gggt
//
LOCUS       DASZYN010000380         4860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_851259, whole genome shotgun sequence.
ACCESSION   DASZYN010000380 DASZYN010000000
VERSION     DASZYN010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851259"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VHE33_09710"
     CDS             complement(<1..514)
                     /locus_tag="VHE33_09710"
                     /inference="COORDINATES: protein motif:HMM:NF042697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HVT97773.1"
                     /translation="MHRTLAIAAFLVSASAFAAPAQQSAPSQPLLPQSFAGFSQSSAV
                     PAADDVDAAVLHEYGLTQSAAATYASGSRQLSLHAYRFGDATGAYGAFTFLRQPGMHA
                     EAVGHDGAASGDHHLFWSGTTVIDATFAPPSANEPAVLATLGAQMPQVGGAAGIPPTL
                     PHYLPKAQLEP"
     gene            complement(579..2765)
                     /locus_tag="VHE33_09715"
     CDS             complement(579..2765)
                     /locus_tag="VHE33_09715"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HVT97774.1"
                     /translation="MLAALCFATAPATFAAQSSSAFARAERLHEALEGRPEQQRTRLQ
                     YEHVLDAYRAIYHGDPGSPKADASIAAVADLLAEEGRVFEDQKALRDAIGQYEFLRHQ
                     YPGTRFRFSALLTEAEIYQRDLGDLTHAKAAYQNFLHLYPQNALAVEARAQLKNIHRE
                     EIAARHHRSSSSRTTAESGESSPPSSHAASGTREHRGSAIAERDNTGESPAAPADAQP
                     VSASDTRLLNAPPPPRRGGLPYITGVRHWSTPTYTRVAIDLQQQVRYEAARVTSPDRI
                     FFDLYGTRLSPELSGRPVEITGDPFLTRVRAAQTSPNTTRVVLDVTSVADYSAFFLQN
                     PPRLIIDVHGNPNPPRPIAAATPPENPSSSGVRAAPVRTPSISERPLPPLPNTSAGGN
                     ESGSASDSDNGSALTDVSKLSRQPGQVRATRHPTTAPVVASVPAAAPSSRSASRRDQV
                     EPSSNDDDDVPPAEAGAGGTPATESATRHAPNSMVRALGLKINRIVIDAGHGGHDSGT
                     LGPGGLQEKDIVLDVALRLGRLLQQRIGADVTYTRSDDTFIPLETRTAIANQAQADLF
                     ISIHANSSPDSDARGVEVYYLSFTSSADALEVAARENATSDESIHQLSDLVKKIALQD
                     KINESRAFADDVDSSLYAGLDQGNPGLKDRGVKKAPFVVLIGANMPSILAEISFLTNP
                     DDARELRQPKYRQRIAEALYRGVSRYVSSLNGVRVAQAGTHPSSSE"
     gene            2915..3058
                     /locus_tag="VHE33_09720"
     CDS             2915..3058
                     /locus_tag="VHE33_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97775.1"
                     /translation="MGLRVRFDALRTGTSQGTRIVTLRCATARAQSVGHLERDYLALA
                     QVI"
     gene            3080..3361
                     /locus_tag="VHE33_09725"
     CDS             3080..3361
                     /locus_tag="VHE33_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HVT97776.1"
                     /translation="MVCHYLVKGRVQGVGFRWFVHREAAALELNGWVRNTEDGHVEVL
                     AAGDAESLDDLKQALRKGSRGSRVDAVIEHELDAKEGEGLGPFQIEGAW"
     gene            3367..3909
                     /locus_tag="VHE33_09730"
     CDS             3367..3909
                     /locus_tag="VHE33_09730"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HVT97777.1"
                     /translation="MTPCATDCEPLKTLIRTVPDFPKPGILFYDITTLLKDPKGFATL
                     VDALAQYYIDKDIDLVLGIEARGFIFGPALAYRLNAGFAPVRKPKKLPGPTARVTYDL
                     EYGSDTLEIHVDAIEPGQRVVLVDDLLATGGTMEATIKLVRQLGGEIAGLGFAIELDF
                     LKGRERFRDYDVFSLLHYSE"
     gene            3942..4583
                     /locus_tag="VHE33_09735"
     CDS             3942..4583
                     /locus_tag="VHE33_09735"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HVT97778.1"
                     /translation="MPNRKRLAWSRFAVYALAAFAAVAGAQTINLVTGNDKAEWTRIA
                     IPPTHPVSDIAQWHIDPATKTIVCDGNGGHEWLRFNREFGDFRLHVEWRFAKLDGSPH
                     YNSGVFFRNDADGTIWHQAQTSPGGGYLFGETLIGEKKTSFNLMKEMTENRLKPAGEW
                     NAYDIECRKDTCTLAVNGAVVNTLHTDVVKGYVGLESEGFKIEFRNIEVTELH"
BASE COUNT          902 a         1292 c         1783 g          883 t
ORIGIN      
        1 gcggctccag ttgcgctttg ggcaggtaat gcggcagcgt gggcggaatg ccggcagctc
       61 cgccaacctg gggcatctga gcaccgagtg tggcaagaac cgccggctca tttgcagagg
      121 gcggagcgaa ggtggcgtcg atgacggtgg ttccgctcca gaagaggtga tggtcgccgg
      181 aggcggcgcc atcgtgcccg acggcttctg catgcatacc tggctgacgc aggaacgtga
      241 acgcaccgta ggcaccggtc gcgtcgccga accggtaagc gtgcagcgag agctgccggg
      301 aaccggaagc ataggttgcg gctgccgatt gcgtgaggcc gtattcgtgg aggacggcag
      361 cgtcaacgtc atcggcggcg ggaactgcgg aactttgcga gaagccggca aaggattgcg
      421 gcaaaagggg ctgcgagggc gcagactgct gcgccggtgc ggcgaatgcc gacgccgaga
      481 cgaggaaggc cgcgattgcg agggtccggt gcatcgctcc agcttcgatg atagttggac
      541 tgacaccgtg tccgaaagaa aggtttccct ggctgggtct attccgagct cgacgggtgt
      601 gtgcctgcct gcgcgacccg gacgccattg aggctggaga catagcggga gacgccgcga
      661 tagagggcct cggcaatccg ttggcgatat ttgggctggc gcagctcgcg agcgtcgtcg
      721 ggattggtga ggaaggaaat ctcggcgagg atcgagggca tgttggcgcc gatgaggacg
      781 acgaacggag ccttcttgac gccgcggtct ttgagacccg gattgccctg gtcgagtccg
      841 gcgtaaaggc tcgagtcgac gtcgtcggca aaagcccgcg attcgttgat cttgtcctgc
      901 agggcaattt tcttcacgag atcggaaagc tggtggatcg attcgtcgga ggtggcgttc
      961 tcacgcgcgg caacttcgag cgcatcggca gacgaggtga agctgaggta atagacctcg
     1021 acgccgcgcg cgtcgctgtc agggctggag ttagcgtgga tggagatgaa gaggtcggcc
     1081 tgggcctggt tggcgatggc cgttcgtgtt tcgaggggga taaaggtgtc gtcgctccgc
     1141 gtgtaggtca cgtcggcacc gattcgctgc tggagaagac ggccgagacg gagagcgaca
     1201 tcgagaacga tgtccttttc ctgcaggcca ccggggccaa gggtgccgga gtcgtggccg
     1261 ccgtgtccgg cgtcgatcac gatgcggttg atcttgaggc cgagggcccg aaccatcgag
     1321 ttcggcgcgt gccgggtggc ggactcggtc gctggggtgc cgcctgcgcc ggcttcggcc
     1381 gggggaacat cgtcgtcgtc attggacgaa ggctcaacct ggtctcttcg cgatgcagaa
     1441 cgtgatgacg gcgctgccgc gggcacagat gcgacgacgg gcgccgttgt ggggtgacgc
     1501 gtggcgcgca cctgaccggg ctgcctgctg agtttggaca cgtccgtgag cgcggagccg
     1561 ttgtcggagt cggatgccga tcctgattcg tttccgccgg cgctcgtgtt cggcagagga
     1621 ggcagggggc gctcggagat ggagggcgta cggaccggcg cggcacgaac cccgctcgag
     1681 ctgggattct ccggtggagt ggcggctgcg atcggacgtg gcgggttcgg attgccgtgg
     1741 acgtcgatga tgaggcgggg cggattctgg aggaagaacg cggaatagtc ggccacgctg
     1801 gtgacgtcga ggaccacgcg cgtggtgttg ggcgaggttt gcgcagcgcg gacgcgggta
     1861 aggaaggggt cacccgtgat ttcaaccggg cggccggaaa gctcagggga gaggcgcgtg
     1921 ccgtagaggt cgaagaagat gcgatcggga ctggtgacac gcgccgcctc atagcgcacc
     1981 tgctgctgga gatcgatggc cacgcgcgtg taagtgggcg tggaccaatg gcggacgccg
     2041 gtgatgtagg gcagaccgcc gcgccgcgga ggtggcgggg cgttcagcag gcgcgtgtcg
     2101 ctggcgctga cgggctgggc atcggcggga gccgccggag attcgccggt gttgtcgcgt
     2161 tcggcgatcg ccgaaccccg gtgctcgcgg gtgccggacg cggcgtgcga ggacggcggc
     2221 gacgattctc cggattcagc ggtcgtacgc gaagaggaag agcgatgatg gcgagcggcg
     2281 atctcttcgc ggtgaatgtt tttgagctgg gcgcgcgctt cgacggcgag cgcgttctgc
     2341 ggataaagat ggagaaagtt ctgataggcg gctttggcgt gggtcaggtc gcccaggtcg
     2401 cgctgataaa tctcggcttc ggtgagcaga gcgctgaaac ggaagcgggt accggggtac
     2461 tgatgccgca ggaattcgta ctggccgatg gcgtcgcgaa gagctttctg atcctcgaac
     2521 acgcgtcctt cttcggcgag caggtcggca accgctgcga tggaggcatc ggctttaggc
     2581 gaccccggat cgccgtgata gatggcacgg taggcatcga ggacgtgttc gtactgcagg
     2641 cgcgtgcgtt gctgttcagg ccgcccttcg agagcttcgt ggagacgttc ggcccgtgcg
     2701 aaagcggaag aagactgcgc tgcgaacgtg gcaggggctg ttgcgaagca gagcgctgcc
     2761 agcaaaagaa agcaccggcg gacggtatgc gatcgggggg acaacatcgg agatcgggcg
     2821 tcgcgctgca tagacggcag ggtcggccga gttccaccgg ccatcgttcc acgcggggga
     2881 gagagcgact ctgccgagac tctctcgatt ataggtggga ctgcgcgtcc ggttcgacgc
     2941 gctccgcacc ggtacctctc agggaacccg catcgtcacg ctgcgttgcg ccactgccag
     3001 ggctcagagt gtcgggcacc tggagcgtga ctatcttgcg ctggcgcagg tcatctgaaa
     3061 cactgagcct gccttcctta tggtgtgtca ttacctggta aaagggcgcg tgcagggcgt
     3121 ggggttccga tggttcgtgc accgcgaggc tgccgcgctg gaactgaacg gctgggtgcg
     3181 caatacggaa gatggtcatg tggaagtgct ggcagcaggc gatgccgagt cgctggacga
     3241 cctcaagcag gctttgcgca agggatcgcg cgggagccgg gtagacgctg tgatcgaaca
     3301 cgaactggat gccaaggaag gcgagggcct cggcccgttt cagattgaag gagcgtggtg
     3361 aaaacgatga cgccttgtgc aactgactgc gagccattga agacgctgat ccggacggtg
     3421 ccggatttcc ccaagcctgg gattttgttc tatgacatca ccacgctgct gaaggaccca
     3481 aagggcttcg cgacgctggt ggacgccctg gcgcagtact acatcgataa ggacatcgac
     3541 ctggtgctgg ggatcgaggc gcgtggattc atcttcgggc cggcgctggc ctatcggctg
     3601 aacgccggct ttgcgcctgt gcggaagccg aagaagttgc cgggaccaac cgcgcgcgtg
     3661 acctacgacc tcgagtacgg gtccgacacg cttgaaattc atgtggacgc catcgagccg
     3721 ggccagcgcg tggtattggt ggacgatctg ctggcgacgg gcggcacgat ggaagcgacc
     3781 atcaaactgg tgcgccagct gggcggtgag attgccggcc tgggattcgc gatcgagctg
     3841 gatttcctca aggggcgcga gcgattccgg gattacgacg tgttcagtct gctgcattac
     3901 agcgagtgag acggctcgaa gccgtaagga agaagttgga gatgccgaat cggaagcggt
     3961 tggcgtggag cagattcgcc gtctacgccc tggctgcgtt cgctgccgtg gccggcgcgc
     4021 agacgatcaa tctggttacc gggaacgaca aggccgagtg gacgcggatt gccattccgc
     4081 cgacgcaccc ggtcagcgac attgcgcaat ggcacatcga tccggcgacg aagacgatcg
     4141 tgtgcgacgg taacggcggc cacgagtggc tgcgattcaa tcgtgagttc ggcgatttcc
     4201 gattgcatgt ggagtggcgc ttcgcgaagc tcgacgggag tccccactac aacagcggcg
     4261 tgttcttccg caacgatgcg gacgggacga tctggcatca ggcccagacc agtccggggg
     4321 gcggatatct gtttggcgag accctgatcg gcgaaaagaa aacctcgttc aacctgatga
     4381 aggagatgac agaaaaccgg ctgaagcccg ctggcgaatg gaatgcttat gacattgagt
     4441 gccgaaagga cacctgtact ctggctgtga acggtgctgt cgtgaatacc ctgcataccg
     4501 atgtcgtgaa aggttacgtg gggctggagt ctgagggatt caaaatcgag ttccggaaca
     4561 tcgaggttac ggaactacat tagtgtggcg gttccgaagt ctcatcatct tgtcatcccg
     4621 accggaccct gcgcgcagcg aaggggaagc ggagagacct gcttctctcc agcatgaaga
     4681 gattttggaa caaaacccta gatgagctgc tcttcgacca gcgcggccag tttctttgga
     4741 gcggcctgga gatagctcgc tgtgatcagg tcgcgcactt catcccagtc gactttgccg
     4801 cggtcgaggc gcagggcgac ccatccgcgc ggaccaacgt aggcggggag atagaacttc
//
LOCUS       DASZYN010000381         3634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1109635, whole genome shotgun sequence.
ACCESSION   DASZYN010000381 DASZYN010000000
VERSION     DASZYN010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109635"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VHE33_09740"
     CDS             <1..191
                     /locus_tag="VHE33_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97779.1"
                     /translation="LPAHTTWDVAVGKAIAENWSLAVNAVNVANTRVLLDNSLTFGGF
                     HENDPRQIYGQVRYRFHF"
     gene            201..1088
                     /locus_tag="VHE33_09745"
     CDS             201..1088
                     /locus_tag="VHE33_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97780.1"
                     /translation="MALAAPLPTVAARPRSRLWGVPIYWHLLSLDAPTVAVLWAWSFA
                     SAAHQRVPFVSIAVLGVGTWLIYVADRLLDVRSPSHPALRERHFFHERHRRVLFLVSP
                     VVGALLLGLICAMPPAARRDDTILFAISMLYFGGVHLPALRVRRWFPREVAVGILFAL
                     ATAVPAWSALDSKPQLAWFVLLFAGLCTLNCIAIETWERSSNTPRSMTVSAMAICAAV
                     ASIALLTMHGHQLPGEFAICASAFTSAALLFTLDGVHRRWFSRSSWPEDRRHFLLALR
                     VAADAALLTPLFFLSLWHL"
     gene            1076..1843
                     /locus_tag="VHE33_09750"
     CDS             1076..1843
                     /locus_tag="VHE33_09750"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT97781.1"
                     /translation="MASVKQIPQLRVNKVRPFAARDLDLLRNPPPRFDRVARFYRAME
                     YLSFGPLLESCRFHHIPRLLDARRAVVLGDGDGRFVARLLASAPCLSADALDASSAML
                     HLLRERVARQGDLHRLTTTCADARIFGAPSAGYDLVVTHFFLDCLTGQETDRLIARLR
                     PHLVPGARWLVSEFEVPRSGALQRSLAQSVVSGLYAAFRLLTGLRVSQIPAWHTALAR
                     HGFVPRAKHSWLGGLLVSQVWELAAARQPRQTLRAST"
     gene            2169..2693
                     /gene="sixA"
                     /locus_tag="VHE33_09755"
     CDS             2169..2693
                     /gene="sixA"
                     /locus_tag="VHE33_09755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HVT97782.1"
                     /translation="MNLYILRHASAGTRRANPAVDVKRPLDKEGKQQCFLVASFLNAL
                     NVQFDRVVSSPLKRALQTASLVGTETGYDSKIEVSEALAPEASVAKFQDLVRSLMKYD
                     NVMVVGHNPNLTVFFGSLLAPGGRMSTRLRKGAIARIDCTRLPGTLHWLVDPRILRGY
                     AKATKSSRPKTSRK"
     gene            complement(2645..>3634)
                     /locus_tag="VHE33_09760"
     CDS             complement(2645..>3634)
                     /locus_tag="VHE33_09760"
                     /inference="COORDINATES: protein motif:HMM:NF044477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97783.1"
                     /translation="IRRLRQFIHREMRRAMRKVHPERVRLVIGTSGTAAAMFDAGRAL
                     GKGRAKKELVPTQTVRRLAAKLTKMKMGERSAVPGIGPKRAEIIVAGSHVLAEVLEAF
                     ALPGFRYSPMGLRDGILAQMLAEQDPRTSAHRQFEQDRWRAVEEACRRYGIDPRQADP
                     VRQHAAQMFHDLAAVHDLPEEYQTWLEAAAMLRDIGKFLNHQGYHRHAQYIIANSELY
                     GFTPQQRVVTSAIARYQGKSRPEPEGRTMRQVPPEEYEHVRRAVVLLRLAVALNQDRA
                     SDVLKVRVRVYPKRVLLELLPGRTGAELELWSLRKEAAYFREVFGRELFVALA"
BASE COUNT          576 a         1280 c         1046 g          732 t
ORIGIN      
        1 acctgcccgc gcacaccacg tgggatgtgg ccgtgggcaa agccatcgct gagaactggt
       61 cgcttgcggt gaatgccgtg aacgtcgcca acacgcgggt gctgctcgat aacagcctta
      121 cgttcggcgg ctttcacgaa aatgatccgc gccagattta cgggcaggtt cggtaccgtt
      181 ttcactttta gaattgaaga gtggctcttg ctgctcccct ccccacagtc gccgcacggc
      241 cccgctcacg actgtgggga gttcccatct actggcacct gctcagcctg gatgcgccca
      301 cggtggccgt tctctgggcc tggagctttg cctccgccgc gcaccagcgc gtccctttcg
      361 tttcgatcgc cgttctcggc gtcggcacct ggctgatcta cgtcgcggac cggctgctcg
      421 acgtccgctc accctcgcat cctgcgctgc gcgaacggca cttctttcac gagcggcatc
      481 gtcgcgtgct gttcctggtc tcgccggtcg tgggagcgct gctgctgggc ctgatctgcg
      541 ccatgccccc ggccgcacga cgcgatgata cgattctgtt cgccatttcc atgctgtact
      601 ttggcggtgt gcacctgccg gcgttgcggg tgcgccgctg gtttccgcgc gaggttgccg
      661 tcggcatttt gtttgctttg gcgaccgcgg tgcccgcctg gtccgcactg gattcgaagc
      721 ctcagctcgc ctggttcgta cttctctttg cggggctgtg tacgctgaac tgcatcgcca
      781 tcgaaacctg ggagcgatcc tcaaacacgc cccgctccat gaccgtttcc gccatggcga
      841 tttgcgccgc ggtcgcgtca atcgcgctcc tgacgatgca cggacatcaa ctccctggcg
      901 aattcgcgat ctgcgcatcc gcgttcactt cggcagccct tctgttcacc ctcgacggcg
      961 ttcaccgtcg ctggttcagc cgaagttcgt ggccggaaga ccgccggcat tttctgctgg
     1021 cccttcgcgt agccgccgat gcagcgctgc tcacacccct gttcttcctt tctctatggc
     1081 atctgtaaaa caaattcccc aactgcgcgt aaacaaggtt cgcccgttcg cggcgaggga
     1141 cctcgatctg ctccggaatc cgcctccgcg cttcgaccgc gtcgcccgct tctaccgcgc
     1201 gatggagtat ctctccttcg gcccgctgct ggaaagctgc cggttccacc acatcccgag
     1261 gctgctcgat gcccgccgtg ctgtggtcct tggtgatggc gacggccgat tcgtggcgcg
     1321 actgctcgct tccgcaccgt gtctctcggc ggatgccctg gatgcctcat cggcgatgct
     1381 tcacctgctt cgcgaacgcg tcgccagaca gggcgatctg caccgcctga cgaccacctg
     1441 cgccgatgcg cgcatcttcg gggctccttc tgcgggttac gacctcgtgg tcacgcactt
     1501 cttcctcgat tgcctgactg ggcaggagac cgatcgcctc atcgcgcgtt tgcggccgca
     1561 tctggtgcct ggagcgcgat ggctggtctc tgaatttgag gtgccgcgct ccggagcgtt
     1621 gcaaagaagc ctcgcccaaa gcgtggtctc gggactctac gctgcatttc gcctgctcac
     1681 cggcctccgg gtaagccaga ttccagcgtg gcacaccgct ctcgcgcggc atggattcgt
     1741 ccctcgcgcg aagcactcct ggctgggagg tctgctcgtc tctcaggtgt gggaactggc
     1801 cgcagccagg caaccgcgtc agacactcag ggcttccacc taacagtgac cctttctgcg
     1861 caacccgaac tgcagcttcg ctcgattgac cctggaccgg aaccgttccc gggactcccc
     1921 aaacccccgg aacccacccc agcgcccgga ccgccgccgg agcccgatcc ggagccttac
     1981 cctggcccga ttccgacccc gcagcccgtc accctgaact gattcgcggc cgccaattct
     2041 ccaatttgac ccggcaaaac ccgcccggga cgggcgcgaa attaacattc gaggcatttc
     2101 cccattgtta caatcaggtt aaaaggacct ctacgccgag gaacgaagca cgttccggcc
     2161 ttctacccat gaatctctac atccttcgcc atgccagcgc cggcacccgc cgcgccaatc
     2221 ctgctgttga cgtaaaacgc cccctcgata aggaaggcaa gcagcagtgc ttcctcgtcg
     2281 cgagctttct gaacgcgctg aacgtgcagt tcgaccgggt ggtttcgagt cctctcaagc
     2341 gggccctgca aaccgcatcg ctcgtgggca ccgaaacggg ctacgacagc aagattgaag
     2401 tctccgaagc gctcgcgcct gaagccagcg tggcaaagtt ccaggatttg gtccgcagcc
     2461 tgatgaaata cgacaacgtg atggtggtcg gccacaatcc gaatctcacc gtctttttcg
     2521 gatcgcttct ggctcccggt ggccgcatga gcacgcgcct gcgcaagggc gccattgcgc
     2581 gcatcgactg cacgcgcctg cccggcacgc tgcactggct ggtcgatccc cgaattctgc
     2641 gcggctacgc caaggcgacg aagagctcgc ggccgaagac ctcgcggaaa taagccgcct
     2701 ctttgcggag cgaccacaac tccagctcgg cgcccgtgcg cccgggcaat aattccagca
     2761 gcacgcgttt cgggtaaacg cgcactcgta ccttgagcac atcgctggcg cggtcctgat
     2821 tcagcgcgac cgcaagccgc agcaagacca ccgcccgtcg cacatgctca tactcctctg
     2881 gcggcacctg gcgcatggtg cgcccctccg gctcgggccg gctcttgccc tggtagcgcg
     2941 caatggccga cgtgaccaca cgctgctgcg gggtgaaccc atacagctcg gagttggcga
     3001 tgatgtactg cgcgtgccga tggtagccct ggtggttcag gaatttgccg atgtcgcgca
     3061 gcatcgccgc cgcttccagc cacgtttgat attcctcagg cagatcatgg accgctgcca
     3121 gatcgtgaaa catctgcgct gcgtgctgcc gcaccggatc cgcctgccgc ggatcgatcc
     3181 cgtaccgccg gcaagcctct tcgacggcac gccagcgatc ctgctcgaac tggcggtgcg
     3241 ctgaggtgcg cggatcctgc tcggccagca tctgcgcgag gatgccgtcg cgcagcccca
     3301 tcggtgaata gcggaagccc ggcagtgcga aagcttccag cacttcggcc agcacatggg
     3361 agccggccac gatgatctca gcgcgcttcg gtccgatgcc cggcacggcg gagcgctcgc
     3421 ccatcttcat cttcgtcagc ttcgcggcca gccttcgcac cgtctgggtc ggcaccagct
     3481 ctttcttcgc acgtcctttg ccgagtgcgc gtccggcatc gaacatcgcc gccgccgttc
     3541 ccgacgttcc gatcaccagc cgcacgcgct ccggatgcac cttgcgcatg gcgcgccgca
     3601 tctcgcggtg gatgaattgc cgcaatcggc gaat
//
LOCUS       DASZYN010000382         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1109963, whole genome shotgun sequence.
ACCESSION   DASZYN010000382 DASZYN010000000
VERSION     DASZYN010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109963"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            904..>2627
                     /locus_tag="VHE33_09765"
     CDS             904..>2627
                     /locus_tag="VHE33_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HVT97784.1"
                     /translation="MDLYRAPTLILLFVLVAVFGALWLQRRTLPPLRMRAGMATTARR
                     RQLLWLVGWMFAAIQLEMEVFGRTEPGLWLAISRASILMAAVMFLGSMAPQYFSRRFH
                     ILYVVAVATPLMAFTVLVSIDPVPGPNMRIVLLLCVVATMLVGLRWGLQRHLLPIWLN
                     LVMVSLVCGFGVWLTLRQQYEGVLALAQSGILLVTALLFAAAFRRASAGVVFTVGGLV
                     AWSVPVMLAPIVGATAVPILLLRLLNLVKVVAAFGMIVLVLEDEIASNKAGQIRDRRA
                     REEMEKYTEIFLEAMPLEEKSTQFNQICEIIAGASRFSKTATVLRSPDGRFRLAGSAG
                     MDGALAEALDAMARRTSADRIAQIGEKFYTRVLGHLVLLDLTPLLEPGDELQQMNFLA
                     VHAIAIRGRDQELLGTLLLSGLRDPDEPLLTEDVLPLELLVARLGTAREHQLLLRRLL
                     QTERLAGLGQLAGGVAHELNNPLTVITGYAELMTDSEGPTHDQATVILNEARRMKQII
                     ESLVRFRKATPGIRTPLSLETMLQDISQLTRHDLEGAHIALQTRIAPALPPVSADGDQ
                     IRQVFLQIV"
BASE COUNT          462 a          998 c          660 g          507 t
ORIGIN      
        1 gacccggact ctcaataggg tccgaattct gatttggggg agccacccga aaataggcca
       61 ggttcacccc acagcaagta gcggaagcct ttccgagcag aacctcaacc caattcgaag
      121 ctgcgtccgc acgaaggtac cttcctccgt tccattttga aaatctgcca ctgccggggc
      181 cgatcaaccc tgcaggtttc acaccgctgt caccacgact ctgcgcgacg acaccggcgg
      241 ctgcgcggcg tggccaccac aactgagcaa gacggtcgtc cgcctcgcag ttccagccct
      301 gccccacagc attcaaacgc caggttcacc ccacagcaag gagcggagat ctttctgcgc
      361 agaacctcaa cccaattcgt agttgcctca gcacgaaagt acctgtctcc gttccaattt
      421 gaaaaatctg gccacagccg gcgccaattt ccccgcagtt tttgcttcgc tgcccaggac
      481 agctcccagg acagctgcgc gatgtaccgc gacagccgcg cgagacaacc tcccgcctcg
      541 caaaaattgc ctcgcctcga acagcattca agtgccaggt tcaccccaca gcaagcagcg
      601 gaaacctcgg ccgaacaaaa atcagctcga tttgttcagg ttcccattcg aaagtacctg
      661 cccgtgccat tttgaacgcc tccgccttgc aatgcccatg gcgatgcctc agaacccacc
      721 tggcgtcgtg ccgagctact ctcccccgag tccagccaac tccttcaacc gcgcctggcc
      781 ccgtcccggt tcccttcccc gttaaccaaa cgggtgacct aaaaacaaag gcctcctcat
      841 ccaggggatg cattttgacc cacctggccc gacaatggca gtcgaaggaa actgggccac
      901 ttcatggatc tctaccgggc gccgacgctc attctgcttt tcgttctcgt cgctgtcttc
      961 ggcgcgctct ggcttcagcg ccgcaccctg ccccccctgc gcatgcgcgc cggcatggcc
     1021 accaccgccc gccgccgcca gcttctctgg ctcgtgggct ggatgttcgc cgccatccag
     1081 ctcgaaatgg aggtcttcgg ccgcaccgaa cccggcctct ggctggccat ctcccgcgcc
     1141 agcatcctca tggccgccgt catgttcctc ggctccatgg ctccccagta cttcagccgt
     1201 cgcttccata tcctttacgt cgtcgccgtt gccacgcccc tcatggcctt cacggttctg
     1261 gtgtcgatcg atcccgtccc aggacccaac atgcgcattg tcctgctgct ctgcgttgtc
     1321 gccaccatgc tggtcggcct gcgctggggc ctgcagcggc atttgctgcc catttggctc
     1381 aatctcgtca tggtttcgct cgtctgcgga ttcggcgtgt ggctgaccct gcgccagcag
     1441 tatgaaggcg tcctcgccct cgcccagagc ggaatcctgc tggtcaccgc gcttcttttt
     1501 gccgccgctt tccgacgcgc ctccgccgga gttgtcttca ccgtcggcgg tctcgtggcc
     1561 tggtcggtgc ccgttatgct ggcccccatc gtgggcgcca ccgcagtccc catcctgctc
     1621 ctgcgcctgc tcaatctcgt caaagtggtc gccgccttcg gcatgattgt tctcgttctc
     1681 gaagacgaga tcgcctccaa caaagccggt cagatccgcg accgccgcgc ccgcgaggag
     1741 atggagaaat acaccgaaat ctttctcgag gccatgcctc tcgaggagaa gagcacccag
     1801 ttcaaccaga tctgcgaaat catcgccggc gccagccgct tcagcaaaac cgctaccgtc
     1861 ctccgcagtc ccgacggccg cttccgtctc gccggcagcg ccggcatgga cggcgcgctc
     1921 gccgaagccc tcgacgccat ggcccgccgc accagcgccg accggatcgc ccagatcggc
     1981 gaaaagttct acacccgcgt tctcggccac ctcgttctcc tcgacctgac tccgctgctc
     2041 gagcccggcg acgagctcca gcagatgaac ttccttgccg tacacgccat cgccatccgc
     2101 ggccgcgacc aggagctcct cggcactctc ctcctctccg gcctgcgcga tcccgatgag
     2161 cctctgctca ccgaagacgt tctccctctg gaactgctgg tcgcccgcct cggcaccgcc
     2221 cgcgagcatc agcttctcct gcgtcgcctg ctgcagaccg agcgccttgc cggtctcggt
     2281 cagcttgccg gaggcgtcgc ccacgagttg aacaatcccc ttaccgtcat caccggctat
     2341 gccgagctta tgaccgactc cgagggcccc actcacgatc aggccaccgt catcctcaac
     2401 gaagcgcgcc gcatgaagca gatcatcgaa agcctcgtcc gcttccgcaa agctaccccc
     2461 ggcattcgca ctccactctc ccttgaaacg atgctgcagg acatcagtca gctcacccgc
     2521 cacgacctcg aaggcgcaca cattgccctg cagacgcgta tcgcgccggc cctgccgccc
     2581 gtgagcgccg acggcgatca gatccgccag gtcttcctcc agatcgt
//
LOCUS       DASZYN010000383         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1042062, whole genome shotgun sequence.
ACCESSION   DASZYN010000383 DASZYN010000000
VERSION     DASZYN010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042062"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..395)
                     /gene="rpmB"
                     /locus_tag="VHE33_09770"
     CDS             complement(207..395)
                     /gene="rpmB"
                     /locus_tag="VHE33_09770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HVT97785.1"
                     /translation="MAQVCDICGKGPQFGNNISHAHNITKRRWNVNLHPVRAKVGGGS
                     KRLRVCSSCLKAGKVQKA"
     gene            complement(590..778)
                     /locus_tag="VHE33_09775"
     CDS             complement(590..778)
                     /locus_tag="VHE33_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97786.1"
                     /translation="MQRTLQDHIAHLEQKIESLKAQLKQSQGSAEFQKGLTLNLEIAE
                     TSLALFRKAFDLERKISN"
     gene            1167..1742
                     /locus_tag="VHE33_09780"
     CDS             1167..1742
                     /locus_tag="VHE33_09780"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HVT97787.1"
                     /translation="MNASAATPALPRLQSALLRNTLAIALGSLLVAICAHLSVPLWFT
                     PVPLTLQPFAVILLGLLLAPGTAAAALVLYLAEGALGLPVFTPHGPGGMLQLLGPTGG
                     YLLSYPFAAFLVSWLRRRFTATPFVSSLLAATTGDALILLSGAAWLAVIQPHAITAIF
                     TLSILPFLPGEILKVIAAAGISAASRRVRRS"
     gene            complement(1868..>2073)
                     /locus_tag="VHE33_09785"
     CDS             complement(1868..>2073)
                     /locus_tag="VHE33_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97788.1"
                     /translation="GRIFVFDVTSGAADKNGKRSRRMEHYWLCGECSQTLAMEQSADG
                     VKVVPRARGFVQEASLARSAMAS"
BASE COUNT          344 a          776 c          521 g          432 t
ORIGIN      
        1 aaacggaagc ggtggcgcag tgccgccagg ggcaccgcaa cgatgcagcc cgcaaaaaaa
       61 gtccagataa cggtgtgaag aactttgacc gcaatcagcg ccagccgcgc gttcacaaca
      121 aacgccgccc acggctccat catgccgatc gccatcgcag tcgtgcagaa atctgttggc
      181 tactggctac tggctgttcc ctgcccttag gctttctgca ccttgccggc cttcaggcag
      241 cttgagcaga cgcgcagacg cttcgagcct ccgcccacct tcgcccgcac tgggtgcagg
      301 ttcacattcc agcgccgctt cgtaatgttg tgcgcgtgcg aaatgttgtt tccaaactgc
      361 ggacccttgc cgcaaatatc gcaaacctgt gccatgtctt ctccaaaaag acgattcctg
      421 cttgagagga tgtcgtcgaa atttgcggcc cgggacttcc ggcttccgac aaacggtccg
      481 gatcccgcct cagccggcag cgtggcgcct cacagcccac atgccgaatc tacagtttac
      541 cggcaattgt gcctccccgg caatcgtcct cgccggcgtc ggcggcgtct cagtttgaaa
      601 tctttctttc gaggtcgaag gctttacgga aaagggcgag ggaggtttcc gcgatttcga
      661 gattgagggt cagccccttc tgaaattcag cagaaccctg ggattgtttc agctgcgctt
      721 tgagcgattc aatcttctgt tcgagatgag cgatgtgatc ctggagcgtt ctctgcacgg
      781 gggaagccta tcacccggat gcctgtccag cccagcaagg cggccaaccc gctcagtgtc
      841 caacggagac gcgaccagcc gtccctccaa cccggcctgc cgtccagctc tcccttccgt
      901 ttgttatcct ctccccgtgc aagttctgta cggcgtcctc ctcctgagta cgatggcgct
      961 gatctgggtc gctctggccg tggtccggca catccgccgg cagcgcgaag cagcgcgctc
     1021 gcagtcggaa ccccacgacg aaaacccata gcaaggcgcg catcattcgg agccctgagc
     1081 cctgtgccct gagccctgta ccctgagccc tgtaccctga acctgagccc tgtaccccgc
     1141 accgcgaacc ctgtaccctg ttctctatga acgcctccgc cgcgacgccc gctctgcctc
     1201 gcctgcagtc cgctctcctc cgcaacacgc tcgcgatcgc cctcggctcg ctcctggtag
     1261 caatctgtgc ccacctctcc gttcctctct ggttcactcc ggttccgctc acgcttcagc
     1321 ccttcgccgt gattcttctg ggactcctgc tcgccccggg caccgcagcc gcagcgctgg
     1381 tcctctatct cgccgaaggc gccctcggcc tgcccgtctt cacgccgcac ggacccggcg
     1441 gcatgctgca gcttctcgga cccaccggcg gctaccttct ctcctacccg ttcgccgcat
     1501 tcctcgtttc ctggctccgc cgccgtttca cggcaacgcc tttcgtctcc tctctgcttg
     1561 cggccaccac cggcgacgcc ttgatccttc tctccggagc cgcctggctt gcggtaatcc
     1621 agcctcacgc gatcaccgcc atcttcaccc tctcgatcct gcccttcctt cccggcgaaa
     1681 tcctcaaagt gatcgccgcc gccggaatct ccgccgcatc ccgccgcgtc cgccgctcct
     1741 gagtccggcc tgccccctca accggcccga agtagcgcgt ccggatgcca gagccccgtt
     1801 tctcccgcaa aaaacaaaag gcgcccgcaa agggcgcctt tttctactcg gtttttctca
     1861 ggccctctta cgaagccatc gccgatcgcg ccagcgacgc ttcctgcaca aagcctcggg
     1921 cgcgcggaac gaccttcact ccatccgcag actgctccat cgccagggtc tgcgaacact
     1981 ccccgcatag ccagtaatgt tccatcctcc gcgagcgttt tccgttcttg tccgccgctc
     2041 ccgacgtcac gtcgaaaacg aagatccgtc cct
//
LOCUS       DASZYN010000384         1871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_529888, whole genome shotgun sequence.
ACCESSION   DASZYN010000384 DASZYN010000000
VERSION     DASZYN010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529888"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..113
                     /locus_tag="VHE33_09790"
     tRNA            24..113
                     /locus_tag="VHE33_09790"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ser,seq:gga)
     gene            510..884
                     /locus_tag="VHE33_09795"
     CDS             510..884
                     /locus_tag="VHE33_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97789.1"
                     /translation="MSKVRLTFVANCGSVAAGGSLVLDTSTQQFSDVSVFCTGGHHVA
                     HKVSEAKFAPPPSQYPAGTPMLINMGDPNGVVVQLNYAIHGFTSPAIPMAGGFVQGIP
                     FAVNNVQGATPGSGSVVVTSVP"
     gene            complement(1005..>1871)
                     /locus_tag="VHE33_09800"
     CDS             complement(1005..>1871)
                     /locus_tag="VHE33_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin"
                     /protein_id="HVT97790.1"
                     /translation="YFDAFSKVAFEMVDQYHVDLFKFDGTGNADRVFPGSMFDSDFAA
                     AIHLIRELRRREPGLFINLTTGTYPSPFWLLNADSIWRDGDDHNFAGVGTQRQQWITY
                     RDAQTYKNIVQGGPLFPLNSLMLHGMIFAQKARGLNTDPQNDFPDEVLTYFGSGTQLQ
                     EMYITPGALSAADWDILARAAQWSRRNASILEDSHWIGGDPGQLQIYGWAAWSPKGWI
                     ITLRNPSNAAQEYSLDLGKALELPPHARSSFSVRQPFAASSSTAQSWSVEHSVAVRMK
                     PFEVRVYEWSGQ"
BASE COUNT          400 a          547 c          529 g          395 t
ORIGIN      
        1 gctcagagcg gcgccgacga cgcggagaga tggccgagtg gctgaaggcg cacgcttgga
       61 aagcgtgtat gctcgaaagg gcatccaggg ttcgaatccc tgtctctccg ccatagcttg
      121 ggcaaatcgc cgattccaaa cacaaactct tacttgctgg acttgatcga aatccagctt
      181 gtccgtgtcg aatccgtccg tcgaagcctg ccctgctggg ccgtttgcat gacaaagcga
      241 cacgtacatg acgacggcac ttgcgagttg tccgaactct ccaatcatcg aatttgcgag
      301 gcgcgatgcc aggtaaacga attccagcgc gctctgggca aactcaactt ctcgatgaaa
      361 ggcaagctca tattcaccaa actctccggc atctcgctgc tacccggcgg agcacttgaa
      421 atcggatcaa tttgccgcgc cttcagtcag agatcgtggg aacaaaatga gcattgataa
      481 gtgctagact caccgccagg aggattgccg tgagtaaggt ccgcctgact tttgtcgcca
      541 actgtggatc tgttgccgca gggggctctt tggtgttgga caccagcacc cagcagtttt
      601 ctgacgtctc tgtgttctgt accggagggc atcatgtggc ccataaggtt tcggaagcga
      661 agttcgcgcc accaccttcg cagtatccgg cgggaactcc gatgctgatt aacatgggcg
      721 atccaaacgg tgtagtagtc cagcttaact acgctattca cggtttcacc agtccggcaa
      781 tcccgatggc cggcgggttt gttcagggca ttccctttgc cgttaacaat gttcagggag
      841 caacgcccgg ctcaggatcg gttgtcgtta cttccgtgcc ctagcactgg ctgagggaac
      901 acgttagtgc gttgtcaagt ttgtcaatat cagttaagcc gcgtggaatc agcgacatgc
      961 aatgttgtcc ccgccaaggc gaacaacctt gattccccgc gtcgtcactg cccgctccat
     1021 tcatacacgc ggacctcgaa aggcttcatc cgcacggcca cggaatgttc cacactccag
     1081 gactgcgccg tggacgatga agccgcaaaa ggctgacgca cgctgaagct gctgcgcgca
     1141 tgcggcggca gctccaaagc ctttcccaga tcgagagagt actcctgggc cgcattcgac
     1201 ggattgcgca gcgtaatgat ccatcccttc gggctccacg ccgcccagcc atagatctgc
     1261 aactggcccg gatcgccacc gatccagtga gagtcttcca gaatcgacgc gttccggcgc
     1321 gaccactgcg ccgcgcgcgc cagaatgtcc cagtccgccg cgctcaaagc tccaggcgtg
     1381 atgtacatct cctgtagctg cgttccgctg ccgaaatagg tcaggacctc atcgggaaaa
     1441 tcgttctgcg gatcggtgtt cagcccgcgc gccttctgcg cgaagatcat gccgtgcagc
     1501 atgagggagt tgagagggaa gagcggcccg ccctgcacga tatttttgta cgtctgcgca
     1561 tcccggtatg tgatccactg ctgccgctgc gtgccgacac cggcaaagtt gtggtcatcg
     1621 ccgtcgcgcc agatggaatc cgcgttgagc agccagaagg gcgagggata cgttccggtc
     1681 gttagattga taaacaggcc gggctcgcgc cggcgcagtt cacgaattaa atggatggcc
     1741 gcagcgaagt cgctgtcgaa catcgagccg ggaaagacgc gatccgcatt gccggtgccg
     1801 tcaaacttga ataggtcgac atggtattgg tccaccatct cgaaggctac ttttgaaaat
     1861 gcgtcgaagt a
//
LOCUS       DASZYN010000385         3886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_397063, whole genome shotgun sequence.
ACCESSION   DASZYN010000385 DASZYN010000000
VERSION     DASZYN010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397063"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VHE33_09805"
     CDS             <1..265
                     /locus_tag="VHE33_09805"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HVT97791.1"
                     /translation="VVDEEQVASIDLLKIDVELSEREVLDGIREEHWQRIQQVVMEVT
                     DKDHALRTIEEMLHARGFSVATEQEPRLKGTAVYNLFATKRAG"
     gene            complement(262..1803)
                     /locus_tag="VHE33_09810"
     CDS             complement(262..1803)
                     /locus_tag="VHE33_09810"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HVT97792.1"
                     /translation="MVTSNPPTDPAARCAAAEQALLRHPGVERTAVLSQAESLLALVV
                     PAEAYLDEAFDRTTAANAALRKWQKTYDLNQSSKLAVSAPSGFNTIGWDSSYTRSPLP
                     SEDMREWVQTTVDSIRRLNPRSVYEIGCGSGLLLLRVAPQCDRYVAVDFSHEVIRRTR
                     EQLQSMPEVAARVELMERRADDFSGLEPQSFDHVVINSVIQYFPHSGYLSQVIGNAVR
                     LVRDGGHIFIGDVRSLPLLPAFAASVELFQAQDELAVAEIRERALRRLHHTPELVLSP
                     AWFLALRHRFDRIAHVEIALRRGRADNEMTRYRYDAILHVGPRREVSAVHYEPASADP
                     KGQIRSRLQQTSEPFGIHSIPNARIERDRLALDQLRDPHAAGNAVGSRQAIGQRALRG
                     VHPQDLFDLETEYPGLRVHLSWAASRSDGSYDALVIPAGAAPDTAFAAPEPSPSAQLR
                     LANAPGQTGIRAQLMERLLAHCREQRPSSGIPDRVVLVDEIPTQLEAADCASLLARED
                     DGLVP"
     gene            complement(1811..2062)
                     /locus_tag="VHE33_09815"
     CDS             complement(1811..2062)
                     /locus_tag="VHE33_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HVT97793.1"
                     /translation="MSVNDEIRNFVQDLLTRQDDRRPLTDSDSLLMSGRLQSIDAVEI
                     VLFLEERFGIDFAEIGFDREQLDSIDSIAALTQTAATRS"
     gene            complement(2071..3492)
                     /locus_tag="VHE33_09820"
     CDS             complement(2071..3492)
                     /locus_tag="VHE33_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97794.1"
                     /translation="MSAFAAQTEAPTVRTGAWANFRLIPRVETWPAAVSFAQTVPGKL
                     VLLGLFGLGMRFLLIDLTAVAGLTFLFGIITLLPEYRRLVLALAPIFMVVRENLQDPL
                     NLAATLSVIAIGMALYLCVMRWPKSRFARRPIVFLLSGFSLLIVCACYAPQHARASAV
                     LWGFVAVLATYVWFIAYAVTDKSSVPKRDGSLELASLRPLWGSTNTPFPKGAAYLRRI
                     EAKTPEQLAVVQLKGLKLLAWAILLGLGQSLWYRFFHGRLGIPIASQALAMSVHGTPV
                     AWHLRWAAQILAYFELLFSVSIFGHRFIACARFAGFNALRNTYRPLSATTLIDFFNRF
                     YYYFKELLVDFFYFPTFLRYFKKHRRLRTIFATCVTILLGDAFYHITRDIHFFQERGL
                     WGALASYQVEFIYCIALAVGLSISQLRKRGPRPTGFVRGRLLPVCGVGFFYVLISVFE
                     TDTRTFTLAQTVRYFASLFFIHF"
BASE COUNT          865 a         1048 c         1331 g          642 t
ORIGIN      
        1 ggttgtggac gaggagcagg tagcgagcat cgatctgctg aagattgacg tggagctgag
       61 cgagcgcgag gtgctggatg gaatccggga agagcactgg cagcggatcc agcaggtagt
      121 catggaagtg acggacaagg atcacgcgct tcgcacgatt gaggaaatgc tgcacgcgcg
      181 agggttcagc gtcgctaccg agcaggagcc acggctgaag ggaacggctg tctacaatct
      241 cttcgcaaca aagcgcgccg gttagggcac aagaccatcg tcttcgcgcg cgagcagcga
      301 ggcgcagtcc gcggcctcca gctgagtggg aatctcatcg accagaacaa cgcgatcggg
      361 tatgccggac gagggccgct gttcgcggca atgcgcgagc aggcgttcca tcagttgggc
      421 gcggattcct gtctgtcctg gtgcgttcgc gagacgaagc tgcgcggaag gtgatggttc
      481 cggtgctgcg aaagctgtgt ccggcgcagc gcctgcggga atgacaagcg cgtcgtagct
      541 gccgtccgaa cgtgaggcgg cccagctgag atggacgcgc aggccggggt actcggtttc
      601 gaggtcgaag agatcctggg gatgaacgcc gcgcagagca cgctgcccga ttgcctgacg
      661 cgagcctacg gcgttgccgg ccgcatgggg atcgcgcagc tgatcgagcg cgagccgatc
      721 cctttcgatg cgagcgttgg ggatggagtg aatcccaaag ggctcgctgg tttgctggag
      781 acgagaacgg atctgccctt tgggatcggc tgatgccggt tcgtagtgaa ccgccgacac
      841 ttcacgccga gggccgacat ggaggatagc gtcgtagcgg taacgtgtca tctcgttgtc
      901 ggcgcggccg cggcgcagcg caatctccac gtgggcgatg cggtcgaatc gatgacgcag
      961 ggcgagaaac caggccggtg aaagtaccag ttccggagta tggtgcaggc ggcgcagcgc
     1021 gcgctcccgg atttctgcca cggcgagttc atcctgggcc tgaaagagct ccaccgatgc
     1081 ggcgaaggcc ggcagcaggg gcaggctgcg cacatcgccg atgaagatat gcccaccgtc
     1141 gcgaacaagg cgaacagcat tgccgatcac ctgtgagaga tagccggagt gggggaaata
     1201 ttgaatcacg gagttgatga ccacgtgatc gaacgactga ggctcgaggc cggagaagtc
     1261 gtcggcgcgt cgttccatca actcgacgcg cgcggccacc tcgggcatcg actgcagctg
     1321 ttcccgggtg cggcgaatga cctcatggga aaagtccaca gcaacatagc gatcgcactg
     1381 aggagcgacg cggagaagaa gcaggccgga gccgcagccg atttcgtaaa cggaccgggg
     1441 attgaggcgg cgaatgctgt ccaccgtagt ctgcacccac tcgcgcatgt cttctgaggg
     1501 aagcggactc cgcgtgtagc tgctgtccca gccaatcgta ttgaaaccgg aaggggcgga
     1561 gacggcgagt ttcgaagact ggttcaggtc gtatgtcttc tgccatttgc ggagtgccgc
     1621 gttcgcagcc gtcgtgcggt cgaaggcttc gtcgagatag gcttcggcgg gaaccacgag
     1681 ggcgagaaga ctttccgcct gcgacaggac ggccgtccgt tccacgccgg gatggcggag
     1741 cagcgcctgt tccgccgcag cgcagcgcgc ggccggatcg gtgggagggt tgctcgtgac
     1801 cacagtcgtc tcagctgcgg gtagcagcgg tttgggtaag ggcagcgatg gagtcgatgc
     1861 tgtcgagctg ctcgcgatcg aaaccgattt cggcaaagtc aatcccgaac cgttcttcga
     1921 gaaacagcac gatctctacg gcgtcgatcg actgcaagcg gccgctcatg aggagcgaat
     1981 cgctgtcggt cagcgggcga cgatcatcct ggcgagtgag caggtcctga acaaagttac
     2041 gaatctcgtc atttaccgac atcgatgctc ctaaaaatgg atgaagaaca agctggcaaa
     2101 gtatcgcacg gtttgcgcga gggtaaaggt acgggtatct gtttcgaaaa cggagatgag
     2161 gacgtagaag aaacccacac cgcagacggg caggaggcgg ccacgaacaa atcctgtggg
     2221 acgcggtccg cgcttgcgca gctgcgagat gctgagccca acagccagcg caatgcaata
     2281 gatgaactcc acctgatagc tggcaagggc tccccagaga ccgcgctcct ggaagaaatg
     2341 aatgtcacgc gtgatgtggt agaaggcatc gccgagcaga atcgtgacgc aggtggcgaa
     2401 aatcgtgcgc agacggcggt gtttcttgaa atagcgaagg aaagtcggga agtagaagaa
     2461 gtcgacgagc agttctttga agtagtagta gaagcggttg aagaagtcga tgagggtggt
     2521 cgcggagaga ggacgatacg tattgcggag agcattgaag ccggcgaagc gggcacaggc
     2581 gatgaatcga tgcccgaaga tggagacgct gaagagcagc tcaaagtaag ccagaatctg
     2641 cgcggcccag cgcagatgcc acgcgaccgg cgtgccgtgg acgctcatcg ccagcgcctg
     2701 gcttgcgatg ggaatgccga gccggccgtg aaagaagcgg taccacaagc tctggccgag
     2761 gccgagcaga atagcccacg cgagcagctt gagtcctttg agctgaacga cggcgagttg
     2821 ctccggagtt ttcgcttcga tgcggcgaag ataggcagcg cccttgggaa agggagtgtt
     2881 ggtgctgccc cagagcgggc ggagcgaggc gagttccagc gatccatctc gcttcggcac
     2941 ggaagacttg tcggtgacgg cgtaggcaat gaaccagacg taggtggcga gaactgcgac
     3001 gaatccccac aggaccgccg acgcgcgtgc gtgctgcgga gcgtaacacg cgcatacgat
     3061 cagcagggaa aaaccggaga gaagaaagac aatggggcgc cgggcaaatc gggatttcgg
     3121 ccaccgcatc acgcagaggt aaagcgccat gccgatggca atcaccgaaa gagtagcggc
     3181 cagattgagc gggtcctgga ggttctcgcg gacgaccata aaaatgggcg ccagggcaag
     3241 gacaaggcgg cgatattcgg ggagcagcgt gatgatgccg aagagaaagg tcaggccggc
     3301 gaccgccgtg agatcaatga gaaggaagcg catccccagg ccgaacaggc ccaacagaac
     3361 cagtttgccg ggcacagtct gagcgaagga aaccgccgcc ggccaggttt caacgcgagg
     3421 gatgagacgg aaattggccc acgcaccggt gcgtacggtg ggcgcttcgg tttgtgcagc
     3481 gaaggcactc atcagtcaaa acagcagggt tgtcctggac gctgccaggg aaacggtccc
     3541 ggcggccatc gcgcaccgtc gagaggaaag ggctctcctt cacaggataa gcagagaccg
     3601 cggaggttcc gatagtactc acggggatgg ggatagtact ttggttggac ctgtcccctg
     3661 gatcggatcc attccgcttc tccccgcaac gctgccatcc gtcccggcgc attgccggga
     3721 gcgccgcacc gaatcgacac acatgcatgg acctgcgaga ctgggaacgc aatcattccc
     3781 tttcatcgag tccccgacgc tgtgcgatga atcgtcccct gctcacattt ctaagcttca
     3841 tcctgtttgg atttgcgttt ctgcgcggcg ccgcccagca gccgaa
//
LOCUS       DASZYN010000386         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_917135, whole genome shotgun sequence.
ACCESSION   DASZYN010000386 DASZYN010000000
VERSION     DASZYN010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917135"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VHE33_09825"
     CDS             complement(<1..437)
                     /locus_tag="VHE33_09825"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HVT97795.1"
                     /translation="MSTYAAGAAFIAVLVIGLAASHKPLGDYMARVYMGDRHLRVERW
                     VYRATGVNPDAEQRWGTYLRSMLAFSAVSVIFLYLMLRLQGHLPFSQGMKGMEQGQAW
                     NTATSFTTNTNWQSYSGESALGYTAQAVGLAVQNFVSAAVGIAV"
     gene            complement(437..526)
                     /locus_tag="VHE33_09830"
     CDS             complement(437..526)
                     /locus_tag="VHE33_09830"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit F"
                     /protein_id="HVT97796.1"
                     /translation="MTAENLIGLILALGTAVFLVLALLFPERF"
     gene            complement(599..1057)
                     /locus_tag="VHE33_09835"
     CDS             complement(599..1057)
                     /locus_tag="VHE33_09835"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HVT97797.1"
                     /translation="MHRLAVWRRALATCQWDSRVPASIAAGIAFTATLVSATALAAAH
                     AAAPVALGVMVVIVTLSGWLVTWSGAVVTAALAWLMLNGFVTDRYGVLRWHGHRDLVE
                     LAVLFASATTVATVRATRIRRSRRRAMDHLTQQLSELTDAAHRTLGGRHA"
     gene            1506..1787
                     /locus_tag="VHE33_09840"
     CDS             1506..1787
                     /locus_tag="VHE33_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97798.1"
                     /translation="MIETYQAGASAEHVAKEFGLSKGAVIQLLTDAGLIRKRNSPTEA
                     QLLEATRLYVEESWSLERIGAELGFRNTTIHRHFKLRGVPMRRPWEHLR"
BASE COUNT          389 a          652 c          622 g          337 t
ORIGIN      
        1 accgcgatgc cgactgcggc ggacacgaag ttctgcaccg cgagcccgac cgcttgcgcc
       61 gtgtaaccaa gggcggactc acccgagtag gactgccagt tcgtgttcgt ggtgaagctg
      121 gtcgccgtgt tccaggcctg accctgctcc attcccttca tgccctgact gaacggcagg
      181 tgtccctgaa gtcgaagcat caagtagagg aagatcaccg agaccgctga gaacgcaagc
      241 atgctgcgca ggtaggtgcc ccaccgttgc tctgcgtccg ggttcacccc cgtcgcgcgg
      301 tacacccacc gctcgactct caggtggcgg tcgcccatgt agacccgcgc catgtagtcg
      361 ccaagcggct tgtgtgatgc cgcgagacca atgacgagga ccgcgatgaa tgccgcgccg
      421 gcggcgtacg ttgacatcag aatcgctccg ggaacagcag ggcgagcacc aggaatacgg
      481 ccgtcccgag tgccaggatc agaccgatca ggttttcggc ggtcacagct tctccgctcc
      541 ccgcgcgatc agcgccagca gcgcgaagaa tccgatcgtc agcaggatga agatgacgtc
      601 acgcatgccg ccctcctagg gttcggtgag ctgcatcggt cagctcggac agttgttggg
      661 tcaggtggtc catcgcgcga cggcgacttc gtcggatccg cgtcgcccgg acagtggcca
      721 cggtcgtggc gctggcgaac agaacggcca gttccacgag gtcgcggtgg ccgtgccagc
      781 ggagcacgcc gtacctgtcg gtgacgaatc cattgagcat cagccaggcc agcgccgctg
      841 tcacaacggc accactccag gtgacgagcc agccggacag cgtgacgatg acgaccatca
      901 cgccgagggc taccggcgcc gcggcatgag cggcggccag cgcggtcgca ctcacgagag
      961 tcgccgtgaa cgcgatacct gcggcaatgc tcgccgggac tcgagaatcc cactggcacg
     1021 tcgcaagagc gcggcgccaa acggctaacc ggtgcacgtt cccacccctt caaacaggca
     1081 ccgtctggtg ccccgatcga gtgaagatcg gccggaaggg tggctgcaac tttcctgacg
     1141 cgttcctgac gcaccatgac gaaaatcagg cgccgccctg acgccgccct gacgcgcccg
     1201 gttgcgcgag aacggattcg gccgatatcc cccattgggc gcgccacccg acatccggcc
     1261 tgacgaacta gaccgacgaa gaccaagcct tgtgcgggat atgtcctgcg acagagtccg
     1321 ggacgtctcg cgacatcaca tcggtggagg tgctgggacg ttactcgaac ctacggcccg
     1381 ggccccttat caagcgggcg ctccgttccg atcggttgat cgaaacacgc ccggccgatg
     1441 aggtccggga gccagttcgg ccccacaagc tctcactacg tctaactata caagaacgat
     1501 ccagtttgat cgagacatat caagcgggcg ctagcgctga gcatgtcgcc aaggagtttg
     1561 gactcagcaa gggcgccgtg attcagctac tcaccgatgc cggcctgatc cgcaagcgca
     1621 actcccctac tgaggctcag ctcctggaag ccacacgcct ctatgtagag gagagctggt
     1681 cactagagcg gatcggcgcg gaactgggct tccgcaacac caccatccac cgacacttca
     1741 agctgcgtgg cgttccgatg cgtcggcctt gggagcatct gcggtgacgg gaagccagcc
     1801 ctcgtcccgg caatctgcag caatgcccgt gccaactcgg atcgtggtca tagttacatg
     1861 ggtccgtccg actaacggtg taactggtca tgtcggttag agggtgttgc tcgggaacag
     1921 tctgccttcg aaggtgatcg cgaacgcgtt gagggctggc ttccatcggg tgatccaccg
     1981 cgctcgtccg cgcccggtcg
//
LOCUS       DASZYN010000387        18899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1236205, whole genome shotgun sequence.
ACCESSION   DASZYN010000387 DASZYN010000000
VERSION     DASZYN010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 18899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18899
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236205"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(385..3891)
                     /locus_tag="VHE33_09845"
     CDS             complement(385..3891)
                     /locus_tag="VHE33_09845"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HVT97799.1"
                     /translation="MRREQETGRLHPGQRSRWLGWLMAWVVGCWLAAGIASAQATTGW
                     SGVVRFASGQPVAGATVLLNWAASNTPAGGVRTEKTAADGSFTFTGLVPGRYAIAVSF
                     AAVSSSPTMSVEQSVLVDVPGPRAQLTIAEGADGSYAISVSPLQAGSAAGSSSGAGTG
                     GEKLSSESVSSLPLNGRDFSTLLLLAAGTMTDVNGATNFTQQFAVNGQRGVEAVFAMD
                     GADSSDPEMGGSAFANFNVDAIQELQSSSGWLPAEVGRGAAGFTNIVTRSGKEGFHGS
                     VFEFLRNSALDARNYFDYPSIVEPGRIPPFKRNEFGFTNGGPVVLPHIYDGRGRTFYF
                     GQYQGFRQVLGTTQVLPMPTAAERAGVDVVKYPDGSTDTLNVPVNPAIAAVLARYPMP
                     NNPTGAYGARTYAAPSNVVTNADQFSIRVDQQLGAKGQFLARFNFDNLTGPTTNPDQT
                     TIDKTFGVEYQDRQRMVEFRYTRTQSPHFSWESIFSITRTTPGFPTPNRTDPALKFND
                     GLYEGFDVAGGSVMQAYGNLFQGQQNFTWTHGRHTWKWGIEARVNRDTTYFGISPNGE
                     YNFGGGTVYSPTFIPSASGTHNVNPGDPLPNTLASLLVGYPYSYTVAVAPPEVSSGAH
                     IGPAAINRNDVNAFFQDTWKINDRWTLDYGLRYEVYTPISERANRTSSFLNVSPPAGQ
                     QQIYVINPQPRYRSGWNGWGPRLQISWNAPHDLRVHAGGSITVIPPNIWQDNFLTGST
                     PFAVYPRVNASRAGEIAYGFQITPDQLPQTYTLKGENVFTTWPLKKLPANTTMDVDRY
                     QHDLAALSPSHQLSLLSISAIDRRFGDAFLQTWNLGLEKTIFGLTADAGYVGTESMRL
                     PRIAPTNAYPGADPGFAPYTTFDNTGAVTGGFGFEQVIEATAHSSYHALQTSLSGTVK
                     HGGPGIQAGYTWAKSLDDVSGVVGGTGSTGAVVVGFPQNPFDNHPEKGPSTFDVTHGF
                     SVSAAQDLHLQTVGWLSGPRMRRWSQGWEVLSISSINSGAPFTVYSGIQQTGYGTSGT
                     DRPDQVAKPQLSTAGSTGRRREDYFGRGADNASFFSIPIGVPGGSGPNAGRFGTLGRN
                     TFRGPAYYDFDSAVIKDTRIGQRRNGVEAADLQFRAEFFNMFNIVNMGLPANTLQGSG
                     FGVISKTAGTSRQIQFSLKIIY"
     gene            complement(4187..6913)
                     /locus_tag="VHE33_09850"
     CDS             complement(4187..6913)
                     /locus_tag="VHE33_09850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT97800.1"
                     /translation="MLSEIWTRTKYLFAGKRRSEVDEEIAFHLQREAEENMARGMTPE
                     EARRRAAVDFGGSERAREQCREQRPVWTLESVWRDVRYGARGLARNPMFTAVAVLTLA
                     LAIGANTTIFSLLDQALMQALPVRNPGELVVLSYAGSGEGHTESHGGDSQGHKHEFSY
                     PMYKDLRNRNTVFSGLIGAAPASVGVVWNNRAERVPAEMVSGNYFDVLGVQPAMGRLF
                     VASDETAPGANPVAVLNFDYWKSQLAEAPVVGKTLLVNGAPFTILGVSAPGFHSMEWG
                     ALPDVYVPITMQKTIQPDWDFLNDHRSYWMNIVGRLKPGMTAAQALAAINPLFTDLRT
                     EEFTQLHDQSAKARTSFITKAHLNLEGGAKGFSPMRDSVETPLTIIMGMVLLVILMAI
                     VNVASLLLVRAAARAREFSVRYALGATSGQVLRQLLSEGMLLGLAGAAIGLALAPEAL
                     RLLIRWMSTGDAGQYAFSATLDWRVLLFTILATLIASLLFSLAPAAQFWNPKLAEALH
                     QGRGGTGGSLRFRRTCVALQIGFSLLLIVAAGLFVRTIDNLRNVNAGFATDHLLAFDL
                     DPSLAGYPSTGVVSVEQRALDAIAALPGVRGAGATNDEDLANDGVSGDVLVAGYTAPP
                     DEEFDVELPWVSDGYLQTLGIPLLVGRYFNAGDTATSTKVAIVNESFAKHFFGSAGAA
                     LGHHVSRPRRPATDALIVGVVKDVKHASVREPAHPTSYTLFAQAERTTGLIYYVRTWQ
                     DPDAAANGIRAAIANVDSKLIVAHLTTMKNEIDEDLLSERTIALLATAFGVLATVLAG
                     IGLYGILAYSTAQRTREIGIRMALGARRMTVVRLILWEVMVLAGGAMLVTIPIAILAT
                     RAVRSQLFGVSIADPLVYGAGIAIICLVAGLAGFVPARRAATVDPARALRTD"
     gene            complement(6929..7258)
                     /locus_tag="VHE33_09855"
     CDS             complement(6929..7258)
                     /locus_tag="VHE33_09855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVT97801.1"
                     /translation="MPPPSDLLQGTLDMLILKSIALKPEHGWAIAQRIQQVSNDVLQI
                     GQGSLYPALHRLEYKGWIRAQWGASENNRRARFYSLTAAGRKQLDAELENWERLTGAV
                     NLVLQRT"
     gene            7448..8389
                     /locus_tag="VHE33_09860"
     CDS             7448..8389
                     /locus_tag="VHE33_09860"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVT97802.1"
                     /translation="MKRWVRIAIRLTAIGTGAWLLASGIAGWNLAQPALHPSHSNRSP
                     VAPAWLHFQPKDPSIQAADGAQLQAWYVRPAHPNGAAVILLHGMADSRLGVAGYASMF
                     LRQGYAVLLPDSRAHGASGGDFATYGVLERDDIRHWAAWLKPQTPGCEYLFGESMGAA
                     IAIQASALTPAVCAVTAEASFASFREIANDRISQVTHLGLWFPRTFGAPAREFAFLYG
                     ILRFHVNLADASPLTAIRLSHVPTLLICGTADDNIPMRHSLELFQAGASHSQLWIVEG
                     AEHTGAAAVNPPLFEQRVVGWFDQHSRPGSRTVVPDQ"
     gene            8458..9063
                     /locus_tag="VHE33_09865"
     CDS             8458..9063
                     /locus_tag="VHE33_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97803.1"
                     /translation="MVSRYRLWPGLLSLGVFLFCTASQAQKILHHDEVSIGGFYQFTS
                     NASGNSITDTPTKSLGGEAAFSHSFLPLMGWQFSYDYTRFTEKYTDRPFGYQHNQHVF
                     NGDYYLHVPAPVFRPYAMAGISAIVFSPSLNGGQNVSWQAAPGANFGAGVDIPLLTGN
                     FGVQVQYRGLYYKAPDFGLAQLKTDSWRLTSQPTAGIYLRF"
     gene            9086..9424
                     /locus_tag="VHE33_09870"
     CDS             9086..9424
                     /locus_tag="VHE33_09870"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HVT97804.1"
                     /translation="MDCIFCKIVAGNIPSKKVFEDEQIFAFHDIDPKAPKHILVIPKK
                     HITSLADAKPEDASLMGRLLLAVADIARDQGLAKGYRVAISTGPEGGQTVDHLHLHLL
                     GGRQMHWPPG"
     gene            9884..12868
                     /locus_tag="VHE33_09875"
     CDS             9884..12868
                     /locus_tag="VHE33_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HVT97805.1"
                     /translation="MLPVLALLGLLCGMTASLHAQTASFSGSTTTIATDFDGPMGVTT
                     DASGNLFVADNGMTPNACNGNTARPAVCELKRTGPGSYSAPIPLPSGFTCPATVTEPL
                     PCLRGLAVDASGNVWVADFNGGSNGLVYELKPSAGAYTTAPVAVPGPASSGWGAPWGV
                     AVDPAGNVFVGDYGAQTVAEISGTSITAGMPVATTVVNAGLVSQPRGIAVDASDDLII
                     IDGNVDRVVALTPPYTSVFNVNNYGFQGPGDLALDASGNLWLSEFNTASVRELTAASG
                     YQTILSWGSGLNGPVSVWPDADGNILESDNQNSAIKQIATTINFGAVAVGSTSAKQTL
                     AFNFNGAANTMIQAPVVVTQGVTGLDFVNAGSGSCTTAANPWAPQTTCTVDVTFKPKF
                     PGARYGAVKLLDTTGNLLATAYIYGTGLAPQLVFPDNTALQTLGGGFNAPLGGAVDAA
                     GNVYVADTANDLVKQIPPGCSSSACVKTLGGGFDSPMGVAVDGSGNIYVADEGNDAVK
                     VMSPACTSSSCVSTLGDGFAGPQYVAVDRSGNVYVTDLLDSGSGVVKKMAPGCASASC
                     VTTLPGDFQSPEGVAVDGIGNLYVLDNGNASVMEIPAGCKSDSCVTTWGDPGGYDGIL
                     NIATDGSGNVYVGDMAVDQSGNVYLADSDNNAVEVLNMLTPPTMNFASTNVGSQSSPL
                     AVTLGNIGNMPLVFPAPPTGFNPSVPAGFMWDNASTCARTASGGSAFSLAPAGTCSVQ
                     IDFQPTASGAITGSVVLANNNLNVASSTQAIGVRGTGVAVATVGISPSSIDFGTLYMG
                     SLATKTVTVTNTSKANMTINDPFLGVVRGGNSSEFITLNLCPRTLAPGRSCTMLVAFI
                     AGPYYTPQTATLAINTNLGSPQTVPLTATVINPAAQFSASSLNFGTVKTKSSSTAKSV
                     IVTSVGGTALSISKVSITGANPGDFSQTSTCTSANLNPKASCSIVVTFKPTAKGTRTA
                     NLVVTDNARKSTQSISLLGKGN"
     gene            complement(13040..13588)
                     /locus_tag="VHE33_09880"
     CDS             complement(13040..13588)
                     /locus_tag="VHE33_09880"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HVT97806.1"
                     /translation="MKTAFALLTFTCALIGAPFAEPQTTAAPAKQLMIELEVPAPLPA
                     VWEAFSTSAGLRTWLGPNATVDLRPGGDWMVRSPDGKSGGGTIVSFVPEQEIVIAAMA
                     PEQFPTVRAQRTRAVFQFEAHGDATVVRLIQTGWKQGPEWDHAYEYLVAGNAELLSML
                     HRRFLEGPLHWKSGDSGIGKKN"
     gene            complement(13623..14021)
                     /locus_tag="VHE33_09885"
     CDS             complement(13623..14021)
                     /locus_tag="VHE33_09885"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HVT97807.1"
                     /translation="MTFAILNVSAILTAALSAFALGALWYAPFVFGAVWKRANGFTTD
                     PPPRATIFLLTFFFNLVMAVNLAIFLNSPKTTTAFGAAAGFAAGLGWAAMGLAIVALF
                     ERRPWSYILVNGGYLAVALTLMGTILGAWR"
     gene            14155..14745
                     /locus_tag="VHE33_09890"
     CDS             14155..14745
                     /locus_tag="VHE33_09890"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT97808.1"
                     /translation="MPSTRERLIDSARFLFWERGFAGTSMADLLAHAGVNSGSFYHFF
                     DSKEALLREVLQGYIDLLRPMVVDPAFASVEEPVARIFAILAGYRQRILATDSKYGCP
                     LGRLALEIDPENAPAHALIARNFQGWIDAIRECLDAMKDRLPAGTHLDALATFVLVSM
                     EGGVMLSRSYRSVEPFDRAIAQLQEHFRLLLSAHER"
     gene            14870..17374
                     /locus_tag="VHE33_09895"
     CDS             14870..17374
                     /locus_tag="VHE33_09895"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HVT97809.1"
                     /translation="MISETAATTLAQAGSTKGQRTAPGVEALAQSFLDNLLFVQGRSV
                     ERATVNDLYMALAHTVRDRLVERWISTTKNYQAQDVRVVCYLSAEFLTGPHLANTLIN
                     LGIYDEAEQAMRHLGLDLNTLIEQEGEPGLGNGGLGRLASCFMDSLATLDIPAIGYGI
                     RYEYGIFDQQIRDGWQVESTDNWLRLGNPWALERPEDAFEVKMGGHTERHMDARGRLH
                     VQWIPERMVRGVPNDTPILGYRTNTANTMRLWSAQAVESFNFGVFNHGDFFGAVADKV
                     ASENLSKILYPNDEGAQGKQLRLAQQYFFVSCSLQHILRIQTTQNRPLAELHRNFAIQ
                     MNDTHPAIAVAELMRLLIDEHGMEWEPAWHVTVQSLSYTNHTLMPEALEQWPLDLFST
                     LLPRPMEIINEINRRHLEDVREKFPGDEERVRRLSLISENGDGKKYVRMAYLASLGSH
                     AINGVAALHTELLKTSVLGDFYALSPEKFSNKTNGVTPRRWMVLSNPRLADLLTSRLG
                     TGWVYDLEKLRRLEPLADDADFRQEWRSVKRHNKLQLSGWIRDRLGVTTEPESMFDVL
                     VKRLHEYKRQHLQVLHILTLLKRIRRDPNAQVAPRTFIFGGKAAPGYTTAKLIIKLIH
                     SVGEVVNNDALVAGRLRVVFLPDFNVKLGQRVYPAADLSEQISLAGKEASGTGNMKFA
                     MNGALTIGTLDGANVEIRKEVGVDNFFLFGMTTPEVEQRRRDGYNPRAIYESNSDLRE
                     VLDALSDGEFSHGDKKLFEPLVTSLLTRDEYMLLADYQTYIDCQDRVSMAFSDAEHWT
                     RMSILNVARIGKFSSDRAIRDYCADIWKTWPVKIHV"
     gene            complement(17452..17787)
                     /locus_tag="VHE33_09900"
     CDS             complement(17452..17787)
                     /locus_tag="VHE33_09900"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVT97810.1"
                     /translation="MTRKDPVLDRLFHALGDPTRRAILDRLSQAPTTVTRLAESQQIT
                     LTAVGQHLAILEECGLVRTEKLGRVRTCSIDAAGFNRLEQWIRDHRSTWERRLDRLGA
                     LLDEEDKPS"
     gene            complement(18033..18503)
                     /locus_tag="VHE33_09905"
     CDS             complement(18033..18503)
                     /locus_tag="VHE33_09905"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HVT97811.1"
                     /translation="MDITTAHSTFVIEHNYSHPPERLYAAFADPEKKRRWYGDRDTPE
                     TSVFEMDFRVGGRERTVYHLGPNTPFPGVALTNLTTFEDIVPNQRIVMSSTMVIGDHR
                     MSASLVTFEFLPANGGTELRCTHQGAFYEGSDGPERREAGWRGLLDRFAKELER"
BASE COUNT         3569 a         5779 c         5796 g         3755 t
ORIGIN      
        1 tccaagcaga attttgagag ttgggccggg tccgggtgcc ttcgagggct ccagcagacc
       61 cgccgtccac aacatctgat gccactatag cacaagcgat gcagaaaata tgagtgtttt
      121 attgtcaata ttgaaaaaga gaattaaacc gttcattttc tgtttcttat ggtcactcta
      181 atccgctgag tgccgggtgc gacggcgtcc ttgtctcccg actctcgcgg atcgcctccc
      241 cggcagagcg cttccgggag atgcgctcgt gcggtgtttc cgaaatttct atatgcggaa
      301 gagaagcagg tccctccgct tccccttcgc tacgctcagg gtccggtcgg gatgacaagg
      361 gaaaggaact tcagaaacac cacgctagta aatgatcttc agcgagaact gaatctggcg
      421 ggaggttccg gcggtcttgc tgatcacgcc gaagcctgac ccctggaggg tattcgccgg
      481 cagccccata ttcacgatgt tgaacatgtt gaagaattcg gcccggaact gcagatcggc
      541 agcctcgacg ccgttccggc gctgcccgat gcgggtgtcc ttgatgacgg cggagtcgaa
      601 atcgtaatag gccggaccgc ggaaggtatt ccgtcccagc gttccgaatc tgcccgcgtt
      661 gggccctgat ccgcctggca ctccgatggg aatggagaag aacgacgcgt tgtctgcgcc
      721 acgtccaaaa tagtcttccc gacggcgtcc cgtgctgccc gcggttgaaa gctgcggttt
      781 cgcgacttgg tcgggccggt cggtgccact ggtcccgtag cccgtttgct ggattccgga
      841 atagacagtg aagggtgccc cgctgttgat gctcgaaatg ctcaggacct cccagccctg
      901 agaccagcgc cgcatccggg gtccactcag ccagccgacc gtctgcagat gcagatcctg
      961 tgccgcgctc acgctgaaac cgtgtgtgac atcgaaggtc gagggcccct tttccgggtg
     1021 gttgtcgaac ggattttgcg gaaagccaac cacgacagcg ccggtcgatc cggttccacc
     1081 gacgaccccg ctgacgtcat ccagtgattt ggcccatgtg tatccggcct ggattcctgg
     1141 accgccatgc ttcacggtgc cagagagcga cgtttgcagc gcgtgatagg aggagtgggc
     1201 ggtggcctcg atgacctgct cgaagccgaa ccctccggtc acggccccgg tgttgtcgaa
     1261 agtggtgtag ggggcaaagc cagggtcggc ccctgggtag gcattggtgg gcgcaatgcg
     1321 cggcagccgc atcgattccg tgccgacgta accggcgtcg gcggtcagcc cgaagatcgt
     1381 tttctccagc ccgagattcc aggtttgcaa aaaggcgtcg ccgaagcggc gatcgatggc
     1441 gctgatgctg agcagcgaaa gctgatggct gggcgagagc gccgccaggt cgtgctggta
     1501 gcggtcgaca tccatcgtag tgttcgccgg cagctttttg agaggccagg ttgtgaagac
     1561 gttctcgccc ttcagcgtgt aggtctgcgg caactgatcg ggcgtgatct ggaagccgta
     1621 agcgatctcg ccggccctcg atgcgttgac gcgcgggtag accgcgaagg gagtcgagcc
     1681 ggtgaggaag ttgtcctgcc agatgttggg cgggatgacg gtgatggatc cacccgcatg
     1741 aacgcgcagg tcgtgcggtg cgttccagct gatctgcagg cgtgggcccc agccgttcca
     1801 gccggaacga tacctgggct gcggattgat gacataaatc tgctgctgac cggcgggcgg
     1861 agagacgttc aggaagctcg acgtacgatt ggcgcgctca ctgatgggcg tatagacctc
     1921 atagcgaagg ccgtaatcga gcgtccagcg atcgttgatc ttccacgtgt cctggaaaaa
     1981 cgcattgaca tcgttacggt tgatggcggc cgggccgatg tgcgcgccgc tcgaaacctc
     2041 aggcggagcc acggccacgg tataggaata tggatagccc accagcagac tggccagcgt
     2101 attgggcagc ggatcgcctg gattcacgtt gtgcgtcccg ctcgccgatg gaatgaaggt
     2161 cggcgagtag acggtgccgc cgccgaagtt gtattcaccg ttggggctga tgccgaagta
     2221 tgttgtatcg cgattaacgc gcgcctcaat gccccatttc cacgtgtgcc gcccatgcgt
     2281 ccaggtgaag ttctgctggc cctgaaagag gttgccgtac gcctgcatga ccgagccgcc
     2341 cgcaacatca aacccttcat acagtccatc gttgaacttc agcgccggat cggtacggtt
     2401 gggcgtggga aatccgggcg tggtgcgcgt gatgctgaag atcgactccc aggagaaatg
     2461 cggcgactgc gtgcgcgtgt agcgaaactc gaccatgcgc tgccgatcct gatactcgac
     2521 gccgaaggtc ttgtcgatgg tggtttgatc gggattagtg gtcggcccgg tcaggttgtc
     2581 gaagttgaac cgagcgagaa actgtccttt ggcgcccagt tgctgatcga cgcgaatcga
     2641 aaactggtcg gcattggtga ccacgttgga aggcgcggca taggtgcgcg ctccgtaggc
     2701 gccggtggga ttattcggca tcggatagcg cgcgagcacc gcagcgattg cgggattcac
     2761 cggcacgttc agcgtatccg tgctgccgtc gggatacttc acgacatcca cgccggcgcg
     2821 ttcggctgcg gtcggcatgg gcaacacctg ggttgtccca agaacctggc ggaagccctg
     2881 atactggccg aagtagaagg tcctgccgcg gccatcgtag atgtgcggaa gcacgaccgg
     2941 cccgccattc gtgaaaccga attcgttccg cttgaacgga ggaatccgtc ctggttcgac
     3001 gatcgacgga tagtcgaagt aattgcgcgc atcgagcgcg gagttgcgca gaaactcgaa
     3061 gacggatccg tggaagcctt ctttgccgga gcgcgtgacg atgttcgtga atccagcagc
     3121 gccgcgcccg acctcggccg gtagccaccc tgaactcgat tgcagctcct ggatggcgtc
     3181 gacgttgaaa ttggcaaagg cgcttccgcc catttcggga tcgctcgagt cggctccgtc
     3241 catggcgaag acggcttcga caccccgctg gccattgacc gcgaattgtt gggtgaagtt
     3301 ggtcgcgcca ttcacgtcgg tcatggttcc ggcggccagc aacagcaagg tgctgaagtc
     3361 gcggccgttg aggggcaggg aactcacgga ttcgctggac agcttctcac cgcccgtgcc
     3421 ggcaccgctg ctgcttcccg cggccgaacc tgcctgtaat gggctcaccg agatcgcgta
     3481 cgaaccatcc gcaccttcgg caatcgtgag ctgtgcccgg ggccctggca catcgaccag
     3541 cacagactgc tccacgctca ttgtgggcga agacgaaacc gccgcaaaag acactgcgat
     3601 tgcgtaacga ccgggaacca gtccagtgaa ggtgaagctc ccgtccgcag cggttttttc
     3661 cgttcgtacg ccgcctgccg gagtattcga tgctgcccag ttgagaagca ccgttgcgcc
     3721 cgccacgggc tgccctgatg cgaaccgcac cacgccactc cagcctgtcg tcgcctgtgc
     3781 cgaagcgatt cctgcggcca gccagcaacc cacgacccac gccatcaacc agcccagcca
     3841 ccggctgcgc tgccctgggt gcaaacgtcc cgtttcctgc tcgcgtctca tccgtcccga
     3901 acccactccg taagctaaca gggaaaggga cttctgcgcg ttccgctaaa cccggacccc
     3961 gtatcggttg ccaagtataa cgtttgagta gaggggttgg gcagcgattt cttttcgcgg
     4021 gcagttccgt tcgtcacatc gcgttgaaaa aattggagag aggaggtgaa cgacgggaag
     4081 cctccgcggc tgaagcccac ttcctcctgc gcctgttgtg gcacgactga agttgtgccc
     4141 ttttacaaaa acgccggcag attcttcact aaattatggg acgccactaa tcggtccgca
     4201 gcgcccgcgc cggatccacc gttgccgctc gacgagcggg aacgaagccc gccagacccg
     4261 caaccagaca gatgatcgca atgcctgcgc cgtagaccag cgggtcggcg atcgagacgc
     4321 caaagagctg gctgcgtact gcgcgtgtag cgaggatcgc gatcggaatc gtgaccagca
     4381 tggctccgcc agcgagcacc attacttccc acagaatcag ccgcactacc gtcatgcgcc
     4441 gcgcgcccag ggccatgcga atgccgatct cgcgggtgcg ctgggccgtc gagtaagcga
     4501 ggataccgta gagcccgatc ccggccagca ccgtggcgag aacgccgaac gcagtcgcca
     4561 gcagcgcaat cgtgcgttcc gaaagcaggt cctcatcgat ctcgttcttc atggtggtga
     4621 ggtgcgcgac gatcagtttg gaatcaacat tcgcgatcgc ggcgcggatg ccgtttgccg
     4681 ccgcatccgg atcctgccag gtgcgaacgt agtagatgag tcctgtcgtt cgctctgcct
     4741 gcgcaaacag cgtgtagctt gtgggatggg ctggctcgcg cacgctggcg tgcttcacat
     4801 ccttcacgac acccacaatg agggcatcgg tggccggtcg ccgtggccgg ctgacgtgat
     4861 ggccgagcgc cgctccggcg ctgccaaaaa aatgttttgc gaaactctcg ttcacgatgg
     4921 cgactttcgt cgaggttgcg gtgtcgcccg cgttgaaata gcgaccgacc agcagaggga
     4981 ttccgagggt ctggaggtag ccgtcgctca cccacggcag ctccacatcg aattcctcat
     5041 ccggaggcgc cgtgtaaccc gcaaccagca catcgcccga gacgccgtca ttggccaggt
     5101 cttcgtcgtt ggtcgcgccc gcgccgcgca cgcccggcag agccgcgatg gcatcgagcg
     5161 cgcgctgctc cacggatacg acgccggtcg atgggtagcc cgccagcgaa ggatccagat
     5221 cgaaggccag cagatgatcc gttgcgaagc ctgcattcac gttgcgcaga ttgtcgattg
     5281 tccgcacgaa cagccccgcc gccacgatca gcagcaggct gaagccgatc tgcagcgcca
     5341 cgcaggtccg ccggaaccgc agcgagccgc ctgtgccgcc gcgtccctga tgcagcgcct
     5401 cggccagctt cgggttccag aactgggcgg cgggagcgag gctgaagagg aggctcgcga
     5461 tcagcgttgc gaggatcgtg aacaggagca cgcgccagtc cagcgtcgct gaaaacgcat
     5521 actggccggc atcaccggtg gacatccagc ggatgagcag gcggagggcc tccggggcga
     5581 gagcgaggcc gatggccgca ccggccagcc ccagcagcat gccttcggag agcaactgcc
     5641 gcagcacctg tccgctcgtg gcgccgagcg cgtagcggac cgagaactcc cgggcccgtg
     5701 cggccgcccg cacgagcaac aggctcgcca cgttgacgat cgccatcagg atcaccagaa
     5761 gcaccatgcc catgatgatc gtcaaaggcg tttcgacact gtcgcgcatg ggcgaaaagc
     5821 ccttcgcgcc gccttccaga ttcaaatgcg ctttcgtgat gaagctggtc cgcgccttcg
     5881 ccgactgatc gtgcagctgc gtgaactctt ccgtgcgcag atctgtaaag agcggattga
     5941 tggctgccaa cgcttgtgcc gccgtcatgc ccggcttcag gcgccccacg atattcatcc
     6001 agtacgacct gtggtcgttc agaaaatccc agtccggctg gatggtcttt tgcatggtga
     6061 tcggcacgta gacatcgggc agggcgcccc attccatgct gtgaaatccg ggagcggaaa
     6121 cgccgaggat ggtgaacggt gcaccattga ccagaagagt cttaccgacg accggtgctt
     6181 cagcgagctg cgatttccag tagtcgaaat tcaggacggc aaccggattg gcgccagggg
     6241 cggtttcgtc gctggcgaca aagaggcgtc ccatggcggg ctgcactccg agcacgtcga
     6301 agtagttgcc gctgaccatc tccgcgggca cgcgctccgc gcggttattc cagaccactc
     6361 ccacggaagc gggcgccgcc ccgatcaacc cgctgaacac ggtattccgg ttgcggaggt
     6421 ctttatacat cggataggaa aactcgtgct tgtgtccctg ggagtcgccg ccgtgggact
     6481 ccgtgtgccc ttcgcctgat cccgcatagc tgagcacgac gagttcgccc ggatttctca
     6541 cggggagcgc ctgcatgagc gcctgatcga gcaggctgaa gatcgtcgtg ttcgccccga
     6601 tggccagagc cagtgtgagc acggccacag cagtgaacat cggatttcgc gccaggccgc
     6661 gggcgccgta cctgacgtcg cgccagaccg actcgagcgt ccacaccggc cgctgctcgc
     6721 ggcattgctc gcgtgcgcgt tcactgccgc caaaatcaac tgctgcccgt cgacgggctt
     6781 cttccggtgt catgccgcgt gccatattct cttccgcctc ccgttgcaga tgaaacgcga
     6841 tttcctcatc cacttcgctg cggcgcttgc cggcgaagag gtattttgtc cgcgtccaaa
     6901 tctcgctcaa cattgaatac cgccgctgtt aagtccgttg cagcaccaga ttcaccgccc
     6961 cggtcaggcg ctcccagttt tccagctctg cgtcgagctg ttttcggcct gccgccgtta
     7021 aagagtaaaa ccgtgcccgg cggttattct ccgaagcgcc ccactgcgct cgaatccagc
     7081 ccttgtactc cagccggtgc agggccggat agagcgagcc ctgcccaatt tgcagcacgt
     7141 cattcgaaac ctgctggatc cgctgcgcga tggcccaccc atgctcgggc ttcagcgcga
     7201 tcgatttcag aatcagcatg tccagcgttc cctgcagcag atcggacggt ggcggcattt
     7261 gggtctcccc tcgacattcg acaacagagg gttagaatct ccctctccct tgtcgaatgt
     7321 caaggggaac gtgacgaatg gaaagccggc ttcgtcctct ggttcaacct ctcgcgaatc
     7381 ttaagggctt ccgcgtcgcg aaggtggcag ccagaaccgg cagggttccg gccccgtatc
     7441 gttactcatg aagcgctggg ttcggatagc catacgcctt acagccatcg ggacaggcgc
     7501 ctggctcctg gcttccggaa ttgccggctg gaatctcgca caaccagctc tgcaccccag
     7561 ccacagcaat cgatcacccg tagcgcccgc gtggctgcac tttcagccca aagacccatc
     7621 gatccaggca gccgacgggg cacaactaca ggcctggtac gttcgtccgg ctcatcccaa
     7681 tggtgcagcc gtcattcttc tgcatggtat ggcggactcg cgcctgggcg tggcgggcta
     7741 cgcctctatg tttctccggc agggttacgc cgtgctcctt ccggactctc gagcgcacgg
     7801 agccagcggc ggcgactttg caacctatgg agtgctcgaa cgcgatgata ttcgccactg
     7861 ggcggcctgg ctaaaaccgc agacgccagg gtgtgaatat ctgtttggcg agtccatggg
     7921 cgctgccatc gcgattcagg cgagcgcgct cacgccggct gtttgcgctg tgaccgcaga
     7981 agcctccttt gcctcgtttc gtgagatcgc caatgatcgc atttcgcagg tgacgcacct
     8041 gggcctctgg ttcccgcgca cttttggtgc gccggcccgc gaattcgcgt tcctgtacgg
     8101 aatcttgcgc ttccacgtga acctcgccga tgccagccct ctgacagcga ttcgtttgtc
     8161 gcacgttcca acccttctca tctgcggcac cgccgatgac aatatcccga tgcggcattc
     8221 gctcgaatta ttccaggccg gcgcctctca ttcccaactc tggatcgtcg aaggagcaga
     8281 gcatacggga gcggcggccg tgaatccgcc cttatttgag caacgcgttg tgggctggtt
     8341 cgatcagcac tcccggccgg gttcgaggac ggttgtgcca gaccagtgac ttcgccagcc
     8401 gggtgcaccg taaccgtcat ccgcccgtta gtatggaact ggaggttccc cgctcgaatg
     8461 gtctctcgtt atcggttgtg gcccggattg ctttccctgg gcgtgtttct cttctgtacc
     8521 gcctcgcagg ctcaaaagat tcttcaccat gacgaggtct ccatcggcgg cttctatcaa
     8581 ttcacgtcga acgcgagcgg caacagcatt accgacacgc cgaccaaatc tctgggagga
     8641 gaggcggcct tcagccatag cttccttcct ttgatgggct ggcaattctc ctacgactac
     8701 acccgcttca cggagaaata cacggaccgg ccttttggct atcagcacaa ccagcatgta
     8761 ttcaacggcg actactatct gcatgtgccg gcgccggtgt tccggcccta cgccatggcc
     8821 ggcatcagcg cgatcgtttt ctctccatcg ctgaacggcg gccagaatgt gagctggcag
     8881 gctgctccgg gagcaaactt cggcgcgggg gtcgatattc cgctgctcac gggtaatttc
     8941 ggggtgcagg tgcagtaccg cgggctctac tacaaggcgc cggacttcgg cctcgcgcaa
     9001 ctgaaaaccg attcctggcg gctcacctca cagcccaccg ctggaatcta ccttcgtttc
     9061 tgattcgtct tcaggagagg caactgtgga ctgcatcttc tgcaaaatcg tcgccggcaa
     9121 cattccttcg aagaaggtct tcgaggacga acagattttc gcctttcacg acatcgatcc
     9181 gaaggcaccg aagcacattc tggtgattcc aaagaagcac atcacgtcgc tggccgatgc
     9241 aaagccggaa gacgcctccc tgatgggccg tttgctcctt gccgtcgcgg acatcgcgcg
     9301 cgatcaggga cttgcaaaag gctaccgggt cgccatcagc accgggcctg aaggggggca
     9361 gacggtcgac cacctgcatc tgcacctgct cggcggccgt cagatgcact ggcccccggg
     9421 atagtccgtc cgaacgaaga ccctcaggat cgttctcctc catctttcga ttgccatttc
     9481 tgcactaacg ttagccgatt gctgtctgcg gcttagcgcc ggcgctacga ttcctgcggc
     9541 gatgaaggcc gcaagcccgt ttccgatcgg tcccccattg ggtaatccgg caggtgaccg
     9601 gagcggtatc cgcgcgacct cgcagtgatc cagcaggaaa cccatcttgg gattgtggcc
     9661 gagcgccgca gcagtaagaa tctcttcaag tttcacaggt attggttgcg tttttactca
     9721 aggtcaaaca ggccgacggc gcctgttttg actgcgattt tttgcctggt tcaaatcgct
     9781 gccttctcaa caggagagag attcgatgta cacccttctt ggttcccgga gcgaaagctc
     9841 ttccgctcgt cccgttcatt tcgattggaa gtcctgcaga cggatgctgc cggttctggc
     9901 gcttctgggc cttctgtgtg gcatgaccgc ctcgctccac gcgcaaacag cttccttcag
     9961 tggcagcaca acgacgatcg cgaccgattt cgacggaccg atgggcgtca cgacggatgc
    10021 cagcggcaat ctgtttgtcg ccgacaacgg aatgactccg aatgcgtgta acggcaacac
    10081 ggcgcggcca gcggtctgcg agttgaaacg cacgggcccg ggctcgtaca gcgcccctat
    10141 tcctttgccg agcgggttta cgtgtccggc cacggtcacc gagccccttc cctgcctgag
    10201 agggctggct gtcgatgcca gtggcaacgt ttgggtcgcc gatttcaacg gtggctcgaa
    10261 cggactcgtt tatgagctca agccttccgc cggggcctac acaaccgcac ccgtggctgt
    10321 tccggggccc gcttcgtcgg gctggggagc cccgtggggt gtcgcggtcg atccggccgg
    10381 caacgttttt gtgggcgact acggcgccca gacggtcgct gagatttcgg gcacgtcgat
    10441 cacggcgggt atgccggtgg ctactaccgt tgtgaacgct ggtctggtgt cgcaaccccg
    10501 tggtatcgcg gtcgacgcca gcgatgacct catcatcatt gacggcaatg tcgaccgggt
    10561 ggtggctctg acgcctcctt acacctcagt cttcaacgtc aataactacg gctttcaggg
    10621 tcctggagac ctggcgctcg atgccagcgg caatctctgg ctctccgagt tcaacacggc
    10681 ttcggtccgc gagctgacgg ctgccagcgg ctatcaaacc atcctgagct ggggcagcgg
    10741 gctcaacggc cccgtctcgg tctggcccga tgccgacggc aacattctcg aatccgataa
    10801 ccagaactcg gcgattaaac agattgcgac gaccattaac tttggcgcgg ttgctgtggg
    10861 ttccacttcc gcaaagcaga cactggcgtt caacttcaac ggcgccgcca acaccatgat
    10921 tcaggcgcct gtcgtcgtta cccagggcgt taccggcctt gatttcgtca atgccggatc
    10981 cggaagctgc accaccgccg cgaatccgtg ggcgccgcag accacctgca ccgtcgacgt
    11041 gacctttaag ccgaaattcc ccggcgcgcg ttacggcgcg gtgaagttgc tcgacaccac
    11101 cgggaatctc ctggcgaccg cttacatcta tggaacgggg ctggcgccgc agctggtctt
    11161 cccggacaac accgctctgc aaaccctggg cggcggcttc aacgctcctc ttggcggcgc
    11221 cgtcgatgct gccggcaacg tctacgtggc cgatacggcg aatgacctcg tgaagcagat
    11281 tcctccgggc tgctcttcct ccgcctgcgt gaaaacgctc ggtggcggat tcgactcccc
    11341 catgggtgtt gcggtggatg gaagcggcaa catctacgtc gccgatgaag gcaacgatgc
    11401 ggtgaaggtg atgtccccgg catgcacttc ctccagttgt gtctcgaccc tgggcgatgg
    11461 cttcgcggga ccgcagtatg tggcagtcga ccgcagcggc aacgtttatg tcaccgatct
    11521 cctggacagc ggttcgggcg tcgtgaagaa gatggccccg ggttgtgcgt ccgccagctg
    11581 tgtcacgacg ctgcccggcg acttccagtc tcccgagggc gtggccgtcg acggaatcgg
    11641 aaacctctat gtcctcgaca acggaaacgc atcggtgatg gagattccgg cgggttgcaa
    11701 gtctgacagc tgtgtaacca cctggggcga ccccggcgga tacgacggaa tcctgaacat
    11761 tgccacggat ggcagcggca acgtgtatgt cggagacatg gccgtcgacc agagcggcaa
    11821 tgtctatctc gccgactccg ataacaatgc ggtggaagta ctgaacatgc tcacgccgcc
    11881 caccatgaac tttgcgagca cgaatgtcgg atcgcagagc agcccgctgg ctgtcacgct
    11941 ggggaacatt ggcaacatgc cgctggtctt ccctgcgcct ccgaccggat tcaaccccag
    12001 cgtccctgct ggctttatgt gggacaacgc atccacctgc gctcgtacgg cttccggcgg
    12061 ctcggcattc tcgctcgcgc cggccgggac ctgcagcgtc cagatcgact tccagccgac
    12121 agcttcgggc gccatcacgg gctcggtggt tctggcgaat aacaacctga acgttgccag
    12181 ctccacccaa gcgattggcg tccgcggcac aggtgtagcg gtcgcgacgg tcggcatctc
    12241 gccctcgagc atcgactttg gcaccctcta catgggcagc ctcgccacga agacggtgac
    12301 cgtcaccaac accagcaagg ccaacatgac catcaacgat cctttcctcg gggtcgtccg
    12361 cggtggaaac tccagtgaat tcatcaccct taacctctgc ccgcggactt tggctccggg
    12421 ccggagctgc accatgctcg ttgccttcat cgccggaccg tactacacac cgcagacggc
    12481 caccttggcg atcaacacca acctggggag cccgcaaacg gttcccctca ccgccactgt
    12541 catcaaccct gccgcgcagt tcagcgccag cagcctcaac ttcggaaccg tgaaaacgaa
    12601 gagcagcagc actgccaaat ccgtcatcgt caccagcgtg gggggcacgg ccctctcgat
    12661 ttcaaaggtg tccatcacag gtgcgaatcc gggagacttc tctcagacca gcacctgcac
    12721 ctccgccaac ctcaacccga aggcgagctg ctccatcgtc gtcaccttca aaccgacagc
    12781 gaaaggaact cgaaccgcaa acctggtcgt gacggacaac gctcgcaaga gcacgcagtc
    12841 catctcgctg ctcggcaagg gcaactagcc gaactgcctg cagtctgaag agagaaaatc
    12901 ccggtggatc atccaccggg atttctttgt ctgtcgccac ggatggaatc gatcaggtca
    12961 gcggtgcggt gttcctccag cgagccggct gttgaggccg ttagcgaacc tcgctgagct
    13021 tctacgcttt ggcggatcgt cagttcttct ttccaatgcc cgagtcgccg ctcttccagt
    13081 ggagcggccc ctccagaaac cgccgatgca gcatcgaaag cagctcggca ttccctgcca
    13141 ctaaatattc gtaggcatga tcccactccg gcccttgttt ccagcccgtc tggatcagcc
    13201 gcaccaccgt tgcatcgcca tgcgcctcaa actggaacac cgccctcgtg cgttgtgcgc
    13261 gaaccgttgg aaactgctcc ggcgccatgg cggcaatcac gatctcctgt tcaggcacga
    13321 aggacacaat tgttccgcca ccggattttc catcgggaga gcgaaccatc cagtcgccac
    13381 cgggacgcag atcgactgtg gcgttcggac ccagccacgt gcggagtccc gcgctggtgg
    13441 agaaagcctc ccacaccgca ggcagcggcg cgggaacctc cagttcgatc atcaattgct
    13501 ttgccggcgc agccgttgtt tgcggctcgg caaacggggc gccaatcagg gcgcaggtga
    13561 acgtgagcag agcaaacgcc gttttcatcg gtcaaaccct ctctcttcga aagcggcggc
    13621 gttcatcgcc acgccccaag aatcgtgccc attagcgtca gagccacggc cagatatccg
    13681 ccgttgacca ggatgtagct ccaggggcgg cgctcgaata atgcgacgat ggcgagcccc
    13741 attgccgccc agccgagtcc agccgcaaaa cctgctgcgg caccgaatgc cgtggttgtc
    13801 ttcggactgt tcaggaagat ggcgagattc accgccatga cgagattgaa aaagaaggtg
    13861 agcagaaata ttgtcgcgcg aggaggcgga tcggtggtga aaccgttcgc tcgcttccaa
    13921 acggcgccga acacaaaggg tgcgtaccag agtgcaccca aagcaaaggc tgacagggcg
    13981 gctgtcagga tcgccgatac gttgagaatc gcaaaggtca tgctgtcgtt ctccgggaat
    14041 gctggtgccc aggggcgtaa agctggccgg gaggcataat tagttgatcg ttcaagtaaa
    14101 ttattcgcta cactaatgct tgtcaatgct tttcctctcg atgtgaccca atctttgcct
    14161 tcaacccgcg agcgcctgat cgattccgcg cgtttcttgt tttgggagcg cggcttcgcc
    14221 ggcaccagca tggccgatct gctggcccac gcgggcgtca attccggcag cttctaccat
    14281 ttcttcgaca gcaaggaggc cctcttgcgc gaagtgctcc agggctacat cgatctcctc
    14341 cggcctatgg ttgtcgaccc cgcattcgcc tcggttgaag aacctgttgc acgcatcttt
    14401 gccatcctgg ctgggtaccg ccagcgcatt ttggccaccg acagcaaata tggatgcccg
    14461 ctcgggcggc tggctctgga gatcgatccg gagaacgcgc cggctcatgc attgattgcg
    14521 cggaattttc agggatggat cgatgccatc cgcgagtgtc tcgacgcgat gaaggatcga
    14581 ctgcccgccg gcacccacct cgacgcactc gccactttcg tgctcgtctc gatggagggt
    14641 ggggtcatgc tctcgcggtc gtatcgatcg gtcgagccct ttgatcgcgc catcgcgcaa
    14701 ttgcaggagc atttccggct gctgctctca gcgcacgaac gataggtcgc gcttggaggc
    14761 gcggcctcaa tcgttaccac cgttacgttt ccgccgaaat ttcgctgatc ttttgggctt
    14821 ttttgctcct tcgcgggact agtttgggag cagtcgcgag aggtgacgga tgatttctga
    14881 aacggcggcg acgacgctgg cgcaggcagg gtcgaccaaa ggccaaagga cggctcccgg
    14941 cgttgaagcc ctggcgcagt ccttcctcga taatctgctg ttcgtgcagg gtcgctcggt
    15001 ggagcgtgca acggtcaacg acctgtacat ggcgctcgcg cacaccgtgc gcgaccgcct
    15061 ggtggagcgc tggatctcaa cgaccaaaaa ctaccaggcg caggatgtgc gggtcgtttg
    15121 ctatctgtcg gcggagtttc tgacgggtcc gcatctggcg aacaccctga tcaacctcgg
    15181 catttacgac gaggcggagc aggcgatgcg gcaccttggc cttgacctga acacgctgat
    15241 cgagcaggaa ggtgaaccgg gtcttggcaa cggcggcctg ggccggctgg cctcgtgctt
    15301 tatggactcg ctggccaccc tcgacattcc tgccatcggc tatggtatcc gctatgaata
    15361 cggcatcttc gatcagcaga ttcgcgacgg ctggcaggtg gagtcgaccg acaactggct
    15421 gcggttaggg aatccgtggg cgctggagcg gccggaagac gcgttcgaag tgaagatggg
    15481 cggacacacc gagaggcata tggatgcgcg cggccgtctg cacgtgcagt ggatccccga
    15541 gcggatggtg cgcggggtgc cgaatgacac gccgatcctg ggctaccgga cgaatacggc
    15601 gaacacgatg cggctgtggt cggcccaggc ggtggagagc ttcaactttg gcgtcttcaa
    15661 ccatggcgat tttttcggcg cagtggcgga caaagtagcg tcggagaatc tttcgaagat
    15721 cctctacccc aacgacgagg gcgcccaggg gaagcagctg cgtctggcgc agcagtattt
    15781 cttcgtctcc tgcagcctgc agcatattct gcggattcag acgacgcaga accggccgct
    15841 cgctgagctg catcgcaact tcgccatcca gatgaacgat acgcatccgg cgatcgcagt
    15901 cgcggagctg atgcggctgc tgatcgatga gcatgggatg gagtgggaac cggcgtggca
    15961 cgtgaccgtg cagtcgctct cctatacaaa ccacacgctg atgccggagg cgctggagca
    16021 gtggccgctg gatctgttca gcacactgct gccgaggccc atggagatca ttaacgagat
    16081 caaccgccgt catctcgagg acgtgcggga gaaattcccg ggtgacgagg agcgcgtgcg
    16141 ccgactgtcg ctgatctcgg aaaacggcga cgggaaaaaa tacgtgcgca tggcttacct
    16201 cgcttcgctg ggcagtcacg cgatcaacgg cgtggctgcg ttgcacaccg aactgctcaa
    16261 aaccagcgtg ctgggcgatt tctatgcgct gtcgccggag aaattctcca acaagaccaa
    16321 cggcgtgacg ccgcgccgct ggatggtcct gagcaatccg cgcctggcgg acctgctcac
    16381 gtctcgcctg ggaacgggct gggtctacga tctggaaaag ctgcggcggt tagagccgct
    16441 ggccgatgac gctgacttcc gccaggagtg gcggagcgtg aagcgtcaca acaagctgca
    16501 gttgtccggg tggatacgcg atcgccttgg ggtgacgacc gagccggagt cgatgtttga
    16561 cgtactggtg aagcggctgc atgagtataa gcggcagcac ctgcaggtcc tgcacattct
    16621 tacgctgttg aagcgcatac gccgcgatcc caatgcgcag gtggcgccac gcacgttcat
    16681 cttcggcggc aaagccgcgc cgggttacac cacagccaag ctgatcatca aactgatcca
    16741 ttccgtgggc gaagtagtga acaacgacgc cctggtggcc ggtcgtctcc gtgttgtgtt
    16801 tctcccggat ttcaatgtga agctcggcca gcgtgtctat ccggcggccg acctgtcgga
    16861 acagatttcg ctcgccggga aagaggcgtc cggcaccggc aacatgaagt tcgctatgaa
    16921 cggagcgctc acgatcggca cgctggacgg cgccaacgtt gaaatccgca aagaggtcgg
    16981 cgtcgacaac ttctttcttt tcggcatgac cacgccggag gtcgaacagc ggcggcgcga
    17041 tggttacaat ccgcgcgcca tttacgaatc caacagcgat ctgcgcgagg tgttggacgc
    17101 gctgagcgat ggcgaattct cgcatgggga taagaagctg ttcgagccgc tggtcacatc
    17161 attgctgacc cgtgatgagt acatgctgct ggcggactac cagacctaca ttgactgcca
    17221 ggaccgagtg agcatggctt tcagcgatgc ggagcactgg acgcgcatgt cgatcctgaa
    17281 tgtcgcgcgg atcggcaagt tctcgtcgga ccgtgcgatt cgggattatt gcgcggatat
    17341 ctggaagacg tggccggtga agattcatgt ttagggacag ctaagaagcg gtcagctgaa
    17401 gtccggtcag ctaagaagcg gttagctaag aaccggttag ctgaggtccg gtcagctggg
    17461 cttgtcttct tcatcgagca gggcgccgag gcggtcaaga cgccgctccc atgtcgagcg
    17521 gtggtcgcgg atccattgtt ccaggcggtt gaaaccggca gcatcgatgc tgcaggtgcg
    17581 cacacgcccc agcttttccg tgcgcaccag gccgcactct tcgaggattg cgaggtgctg
    17641 gcccacggca gtgagcgtga tctgctgcga ctccgccagg cgggtgacgg tggtgggggc
    17701 ctggctcagg cggtcgagga tcgcgcgccg cgttgggtcg cccagggcgt gaaacaggcg
    17761 gtcgaggaca gggtccttgc gggtcaagaa gctccttgtg gacggacgcc ggtctgatcg
    17821 gagtggcggt gaagccagcg tgtagccgtc gaaggactgg cggccgtcgc acgattgtat
    17881 ttcctcatgc tggtgtggtc tcccataggc ttgtctcaac agggcggaaa agcaggttcc
    17941 tcggctcacc acccccaaac tgaaatgcgt ttggggcccc tttcgctcgg aatgacaagc
    18001 ctctttggca aacaggcttc cgggacacca cgttagcgct ccagctcttt cgcgaagcgg
    18061 tcgaggaggc cgcgccatcc ggcttcgcgc cgctcaggac cgtcggaacc ttcgtaaaac
    18121 gcgccctgat gggtgcagcg cagttcggtt ccaccgttgg cgggaagaaa ttcgaaggtg
    18181 acgagcgagg cggacatgcg atggtcgccg atgaccatag tcgacgacat gacgatgcgc
    18241 tggttgggaa cgatgtcctc gaaggtggtc aggttggtga gcgcgacgcc cgggaagggc
    18301 gtgttggggc cgagatgata gacggtgcgc tcgcggccgc cgacgcggaa gtccatttcg
    18361 aagacgctgg tttcgggggt gtcgcggtcc ccataccagc ggcgtttctt ctccggatcg
    18421 gcgaaggcgg cgtagagccg ttccggggga tgggagtagt tgtgctcgat gacgaaggtg
    18481 gagtgggcgg tcgtgatgtc catggggatc ctgcctttcc tgaatactga agtgcgtgct
    18541 tcagtgttag gatgccccga tctcggggta attgtcaagc ggatacttca gtatttttga
    18601 aatcttgtgc ttgctgcgga gttgcaagct ttaagcgcgg ctgaagccga ttctctccgg
    18661 gctggtgttc catggcctga aggccatggc ttccaccgtc gcctcgcttt gcaaggcctg
    18721 aatgcagacg atttgctcgc cttccgaatc cgattgtccg gttcccggcc gattgccggc
    18781 tattgaaaga gcttgaggtc gatggagtcg cccatctttg cgtaacccgc gtcgttgggg
    18841 tgcaggtggt cgcctgaatc ataggcgggg gcgaaggcgg tgggatttgc cgggtcctg
//
LOCUS       DASZYN010000388         7150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1110262, whole genome shotgun sequence.
ACCESSION   DASZYN010000388 DASZYN010000000
VERSION     DASZYN010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7150
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110262"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1603
                     /locus_tag="VHE33_09910"
     CDS             <1..1603
                     /locus_tag="VHE33_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HVT97812.1"
                     /translation="LNKEYGLKSWLLTGDHKRIAMLYLISITFFFFIGGAMAGLIRME
                     LLTPPSDLLAADTYNKIFTAHGLIMVFFFLVPSVPATIGNFLIPIMVGAKDLAFPKIN
                     LLSWYLYIVAGSMMIAVLAFGGVDTGWTFTTPLSTHYLNTHVITAGLAVFVAGFSSIF
                     TGLNFIVTIHRMRAPGMTWFRLPLFVWSHYAASILMVLGTPVLAITLVLVVLERAIGI
                     GVFDPTKGGDPLLFQHLFWFYSHPAVYIMILPGMGVISEVMSTFSRKRVFGYTAVAFS
                     SVAIAVFGFFVWAHHMFVMGISNYSALVFSLLTMLVAVPSAIKIFNWSATLYKGSITF
                     ETPMLYAFGFIGLFTIGGLTGVFLGTLGMDIHLTETYFIVAHFHFVMVGGMLMAYLSG
                     VHFWWPKMTGRMYPEAMSKLAAVITFTGFVLTFFPQFVLGYLGMPRRYAAYPPEFQVL
                     NVFSTAGASVLGVGYLLPLLYLTWSLKYGKIAGPNPWQATGLEWQIQSPPLTENFAET
                     PIVTHEAYDYEWLESQKPSEVTTVG"
     gene            1596..2336
                     /locus_tag="VHE33_09915"
     CDS             1596..2336
                     /locus_tag="VHE33_09915"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HVT97813.1"
                     /translation="MDSQTLAAQTADTGHVEHEHPPFLRHHFENVPQQREAATFGMWL
                     FLLTEIMFFGGLFCAYLIFRNWYYPAFVAGSHTLHIWAGTLNTTILIISSFTMAMAVH
                     ASETRVRKALIGWMIATIILGCGFLCVKAYEWHDEWVEHHVPGLNFSPESFLHGSKDY
                     PDDKLPLDMAEKTQVYYSLYFAMTGMHALHMIIGVSLLSILLFGAYKGKYVNGHVAPI
                     ENFGLYWHFVDIVWIFLFPLLYLISRHQ"
     gene            2391..2750
                     /locus_tag="VHE33_09920"
     CDS             2391..2750
                     /locus_tag="VHE33_09920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HVT97814.1"
                     /translation="MEQHEGKHHEHHVHLPGPSEPTHHEEHIVSPKIYLLIGTALLIL
                     TATTTAVSYVELGDFNAVVALLIACIKMTLVVLFFMHVKYSSGLTKLTVAAGFFTFIV
                     LITMTMTDYISRAWGRW"
     gene            3020..5521
                     /locus_tag="VHE33_09925"
     CDS             3020..5521
                     /locus_tag="VHE33_09925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT97815.1"
                     /translation="MLRNVLLAIRVLRRNPVFTAIAVLTIALGVGASTAIFSVTNAVL
                     LQPLPYRNPSQLVIGGMDLRKRNVHGLPFSNADFIDLKNGTRGQFSDMAGVFTFQQVV
                     QKADGSPEQINGAVATTNFFSLMGARILYGRDFNESDGIPQPPQPDDGQAQAGAAPAA
                     APPQLPQMAILSYDYFLHRYGGDPHVIGQSVGNGNGPRSVVVGVLAPGFQLYFPPEDN
                     VEAKPDLWIANRLDYDAKNRNDFGIVPVARLKEGATLEQAQSAADVVATEARKSFPID
                     ATAGYYITLEPMRKHLVAEVKPAILALMGSVIFLLLIACANVANLLLVRASLREHEFA
                     IRAAMGANRGRLVAPLFTEACVLALLGTALGLGLAWAGIQELRALAPENLPRLEGVGI
                     SGTVLLFTALAGLVAAVVFGLAPAWRASQPALMNVLRGTSRTSGLASGAFLRNGVVMA
                     EVALSFVLLIGSGLMFRSFLDLQRIDPGFDAKHLLTFQLLQGQFRGKTPEERAAKMRE
                     IRDRLRAIPGVEDVTASFPFPLTGQFGPIRWGTEDAASDASKFQATDFQIVLPGYFEA
                     MRTPLLAGRTFTEDDNQPGRNYVIVDQALARKAFPGQSAVGKRILTRIRTPQAEWVQI
                     IGVVGHQREESLAEPGREQIYFPDAFLKSGVVRSWALRTGSDPARYEDQVRAAVASVS
                     RDLLVLHMEPAETVVREAQSGTRFSLLLIGLFAVIAGVLAGVGLYGVLATAVRQRTSE
                     IGVRMAMGAERGDIVKLVVMQGMRLSVVGIVLGFIAAVALGRVMTSMLVGVKATDPLT
                     YVSMIVIFLAIAMIASWLPAWRAAGLDPKTALHEN"
     gene            5655..5804
                     /locus_tag="VHE33_09930"
     CDS             5655..5804
                     /locus_tag="VHE33_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97816.1"
                     /translation="MIDFLVTEKHLSRDDASMLTSVAADVDITQLVDGNVGVHVICPK
                     GIFRK"
     gene            5864..6172
                     /locus_tag="VHE33_09935"
     CDS             5864..6172
                     /locus_tag="VHE33_09935"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HVT97817.1"
                     /translation="MAPEISSRKAELVEICREHHVRLLEIFGSAAGSAFHPERSDFDF
                     LVEFDAIPEGAYAANFFDLKEALERLLGRPVDLIVPSAIRNPWFRKGIERDRALLYAA
                     "
     gene            6162..6515
                     /locus_tag="VHE33_09940"
     CDS             6162..6515
                     /locus_tag="VHE33_09940"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HVT97818.1"
                     /translation="MRLESRKYLYDIEEAATLASQFTRGKSFQDYRNDAMLRLAVERA
                     FSIIGEALVQLARIDPPSAGRITDHQRIIAFRNILVHAYAHVDNRLVWEIVEYKLPGL
                     MNEVSELMNDPDEPS"
     gene            complement(6573..>7150)
                     /locus_tag="VHE33_09945"
     CDS             complement(6573..>7150)
                     /locus_tag="VHE33_09945"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HVT97819.1"
                     /translation="AKNLLRIAVIDIASGRMTHEYGYLLTSGSGVSEILALNNHEFLV
                     DERDGKGRADGSKAKVKQLFKIDLNGATDIRDMDGTTAAANTVPKALFLDIVKSLTAI
                     GIDPGNIPAKIEGITFGSDIWSSGTRLHTLWVANDNDFLETVADANGNQIPNPNQFFV
                     FGFTDTDLGGSRLVLQQFQDFDRRDDRGFGW"
BASE COUNT         1278 a         1989 c         2336 g         1547 t
ORIGIN      
        1 tctgaataaa gaatacggtc tgaagagctg gctgctgacc ggcgaccaca agcggatcgc
       61 gatgctgtat ctgatctcga tcacgttctt cttcttcatc ggcggcgcca tggccggact
      121 gatccgcatg gagctgctga ctccgccgag cgatttgctg gccgccgata cctataacaa
      181 gatcttcacg gcgcacggcc tcatcatggt gttcttcttc ctggtgccgt cggtgccggc
      241 caccatcgga aatttcctga tcccgatcat ggtgggagcg aaggatctcg cgtttccgaa
      301 gatcaacctg ctgagctggt atctgtatat cgttgccggc tcgatgatga ttgcggtgct
      361 ggccttcggc ggcgtggata ccggctggac tttcaccacc ccgctcagca cgcactacct
      421 caacacccac gtcatcacgg cgggactggc tgtcttcgtc gccgggttca gctcgatctt
      481 taccggcctc aatttcattg tgacgatcca ccgcatgcgc gcgccgggca tgacctggtt
      541 ccggctgccg ttgtttgtgt ggtcgcacta tgcggcgtcg atcctgatgg tgctgggcac
      601 cccggtgctg gccatcacgc tggtgctggt ggttctggag cgggcgatcg gaatcggcgt
      661 cttcgatccc accaagggtg gcgatcccct gctgttccag catttgttct ggttctattc
      721 gcaccccgcc gtgtacatca tgattctgcc cggcatgggt gtgatctccg aagtgatgag
      781 cacgttctcg cggaagcggg tcttcggcta tacggcggtg gcgttctcgt cggtggcgat
      841 tgcggtgttc gggttcttcg tctgggcgca ccacatgttc gtgatgggca tctcgaacta
      901 ctcggcgctg gtgttctcgc tgctcaccat gctggtggcg gtgccctcgg caatcaagat
      961 cttcaactgg tcggcaacct tatataaggg atcgatcacg ttcgagaccc cgatgctcta
     1021 cgcgttcggg ttcatcgggc tgttcaccat cggcggcctg accggcgttt tcctgggcac
     1081 gctggggatg gacattcacc tgaccgagac ttacttcatc gtcgctcact ttcacttcgt
     1141 gatggtgggc ggcatgctga tggcgtacct gtcgggcgtg cacttctggt ggccgaagat
     1201 gacggggcgc atgtatccgg aagcgatgtc gaagctggcc gccgtgatca ccttcaccgg
     1261 attcgtgctg accttctttc cgcagtttgt gctgggatat ctgggcatgc cgcggcgcta
     1321 tgcagcctac ccgccggagt tccaggtgct gaatgtgttt tcgaccgcgg gcgcgagcgt
     1381 gctgggcgtc ggctatctgc tgccgctgct gtatctgacc tggtcgctga agtacggtaa
     1441 gattgccgga ccgaatccgt ggcaggcgac agggctggag tggcagattc agtcgccgcc
     1501 gctgaccgag aatttcgccg agacgccgat tgtgacgcat gaggcgtacg actacgagtg
     1561 gctggagagc cagaaaccga gcgaggtgac caccgttgga tagccaaact ctggcagcgc
     1621 agacggcaga tacgggccac gtggagcacg agcacccgcc cttcctgcgc caccacttcg
     1681 agaatgtgcc gcagcagcgc gaggcggcca cgttcgggat gtggctcttc ctgctgacgg
     1741 aaatcatgtt cttcggcggc ctgttctgcg cctatctgat cttccgcaac tggtattacc
     1801 cggcgttcgt agcgggcagc cataccctgc acatctgggc ggggacgctg aacacgacta
     1861 ttctgatcat ctccagcttc acgatggcga tggccgtgca cgcctcggag acgcgcgtgc
     1921 gcaaggcgct gatcggctgg atgattgcca cgatcattct gggctgcggc tttctgtgcg
     1981 tgaaggccta cgagtggcac gacgagtggg tggagcatca cgtgcccgga ctgaacttca
     2041 gcccggagag ttttctgcac ggaagcaagg actacccgga cgacaagctg cccctcgaca
     2101 tggccgagaa gacgcaggtt tactactcgc tctacttcgc catgacgggc atgcacgcgc
     2161 tgcacatgat catcggcgta tcgctgctct cgattctgct gtttggggct tacaagggca
     2221 aatacgtcaa cggacacgta gcgccgatcg aaaactttgg cttgtactgg cactttgtcg
     2281 acattgtgtg gatctttttg ttcccgctcc tgtatctgat cagtcgacat cagtgacggg
     2341 gcacagagct tggggttcag ggattagagt tcaggaaatt gggagccagg atggagcagc
     2401 acgagggtaa gcatcacgag catcacgtcc acttgccggg gccgtcggag cccacgcatc
     2461 acgaagagca catcgtcagc ccgaagatct atttgctgat cggaacggcg ctcctgatcc
     2521 tgacggcgac gacgacggcg gtttcctacg tagaactggg cgacttcaat gccgtggtgg
     2581 cgttgctgat tgcgtgcatc aagatgactc tggtggtgct cttcttcatg catgtgaagt
     2641 acagctcggg actgaccaag ctgacggtgg ccgccggatt cttcaccttc atcgtgctga
     2701 tcaccatgac catgaccgac tacatcagcc gggcatgggg acgctggtag ggactcgccg
     2761 tccaggcaga cgcgcttcgc gcaaagcgac tcaacgaggg gagcagctta ggctggtccc
     2821 ctttcgtttt tgacgaggcc tggcgcgctt catcatcggg attcgttttc gtcacaaaag
     2881 gccggtcagg gccgatgtcc tgggaggata atgtttccac gcaaccccag gatttggagt
     2941 cggctcgcgg gcttgcgtcc ccagggctac tgatctgatc tgacaatctg attgccgttt
     3001 cgaacgggcg aggttcccta tgctcaggaa tgtgctgctt gcgattcggg ttctgcgcag
     3061 gaatccggtt ttcacggcga ttgcggtgct gacgattgcg ctgggtgtgg gcgcgagcac
     3121 cgcgatcttc agcgtgacga atgcagttct gctgcagcct ttgccgtacc gtaatccgag
     3181 ccagctcgtg atcggcggga tggacctgcg caaacgcaat gtgcacgggc tgcccttctc
     3241 gaacgccgat ttcatcgacc tgaagaacgg cacccggggg cagttctccg atatggccgg
     3301 cgtcttcacc tttcagcagg tggtgcaaaa ggccgatggg tcgccggagc agatcaacgg
     3361 agccgtggca acgacgaatt tcttcagcct gatgggcgcg aggattcttt acgggcgcga
     3421 cttcaatgag agcgacggga ttccgcagcc tccacagccg gacgacgggc aggcgcaggc
     3481 aggggcggcg cctgcggcgg cgccgccaca gctgccgcag atggccattc tgagctatga
     3541 ctacttcctg caccgttacg gcggcgaccc gcatgtaatc gggcaaagcg tgggaaatgg
     3601 aaatggtccg aggtcagtcg ttgtgggcgt gctggcgccg ggattccagc tgtattttcc
     3661 gccggaggac aatgtcgaag cgaagccgga tctttggatt gcgaatcggc tcgactacga
     3721 cgccaagaac cgcaacgact ttggtattgt gcccgtcgcg cgactgaaag agggcgccac
     3781 gctggagcaa gcgcagagcg cagcggacgt ggtggccacc gaagcgcgaa agagcttccc
     3841 catcgacgca acggcgggct actacatcac gctcgagccg atgcggaagc acctggttgc
     3901 ggaggtgaag ccggcgatcc tggcgctgat ggggtcggtc atcttcctgc tgttgattgc
     3961 gtgcgccaac gtagcgaatc tgctgctggt gcgggcgtca ctgcgcgagc acgagtttgc
     4021 gatccgggcg gcgatgggcg cgaatcgcgg gcggctggtc gcgccgctgt tcaccgaagc
     4081 gtgtgtgctg gcgttgttgg gtacggctct gggccttggc ctggcctggg caggcattca
     4141 ggaattgcgc gccctggcgc cggagaattt gccgcgtctg gaaggcgtgg ggatctcggg
     4201 aaccgtgctg cttttcacgg ctctggccgg actggtggcc gccgtggtgt tcggtctggc
     4261 tccggcatgg cgcgcctcgc agccggcgct gatgaatgtg ctgcgcggaa ccagccgtac
     4321 ctccggactg gccagcggag cctttctgcg caacggcgtg gtgatggccg aggtggcgct
     4381 gtcgttcgtg ctcctgatcg ggtcgggtct gatgttccgc agttttctcg atctgcagcg
     4441 gatcgatcct ggcttcgacg cgaagcattt gctgaccttt caactgctgc aggggcagtt
     4501 ccgaggcaag acgccggagg agcgcgcagc gaaaatgcgt gagatcaggg atcgcctgcg
     4561 ggcgattccc ggcgttgaag atgtaacagc gtcgtttcca tttcccctga ccggccaatt
     4621 cggccccatc cgctggggaa cggaagatgc agcgagcgac gcgagcaaat tccaggcgac
     4681 cgatttccag atcgtgctgc cggggtactt cgaagcgatg cggacgccgc tgctggcggg
     4741 ccggacgttt accgaggacg ataatcagcc gggacggaat tacgtcatcg tcgatcaggc
     4801 tctcgcgcgg aaggcgttcc ctgggcagtc ggccgtgggg aagagaatcc tgacgcggat
     4861 tcgcacgcca caggcggagt gggtgcagat catcggcgtc gtggggcatc agcgcgaaga
     4921 atcgctggcg gagccagggc gcgagcagat ttacttcccc gacgcatttc tcaagagcgg
     4981 cgtcgtacgc tcctgggcgc ttcgcaccgg aagcgatccg gctcgctatg aggatcaggt
     5041 gcgcgcggcc gtggcgagcg tgagccgcga cctgctcgtt ctccacatgg agccggcgga
     5101 gaccgtggtg cgcgaggcgc agtcgggcac gcggttctcg ctgctgctga tcgggttgtt
     5161 tgcagtcatt gcaggagtgc tggccggagt gggcctgtac ggagtgctgg cgacggcggt
     5221 acggcagagg acctcggaga tcggcgtgcg catggccatg ggtgcagaac gtggcgacat
     5281 cgtgaagctg gtcgtgatgc agggcatgcg tctgagtgtc gtcgggatcg ttctgggatt
     5341 catcgcagcc gtagcgctgg gtcgagtgat gacgtcgatg ctggtcgggg tgaaggcgac
     5401 cgatccgctc acctatgtga gcatgatcgt gatcttcctc gcgatcgcga tgattgcatc
     5461 gtggctgccg gcgtggagag cggcgggact ggatccgaag accgccctgc acgagaactg
     5521 aagggcagcg aatagcgtgt agcgttcagc gggtcgcaca ggatttgcgc gtcagggtgc
     5581 gcggtgttgc cttctgctgt gagccgcgga cctgttgaat tctgaaaacg gcgacagaga
     5641 tggcggggcg caacatgatc gactttctcg ttacggagaa gcacctgtcg cgcgatgacg
     5701 cctccatgct caccagcgtg gcggcggatg ttgacatcac gcagctggtc gacggcaatg
     5761 tcggtgtgca tgtgatctgt ccgaagggga ttttcaggaa gtgagtttat gctcgtgacc
     5821 gctcgcacgg taggatagag tcgtccctga caggagcttt cgaatggccc cggaaatctc
     5881 gtcgcgcaaa gcggaattgg tcgagatctg ccgcgaacat cacgtgcgac tgctggagat
     5941 cttcggatcg gctgccggat cggctttcca tcccgaacga agcgatttcg attttctggt
     6001 cgagttcgat gcgatcccgg agggcgcgta tgccgctaat ttcttcgatc tgaaggaggc
     6061 gctggagcgc ctgctgggcc gccccgtgga tctcattgtg ccgtctgcga ttagaaatcc
     6121 gtggtttcgc aagggaatcg agcgagacag ggcactgctt tatgcggctt gaatcccgca
     6181 aatatcttta cgatattgag gaagccgcga cgctcgcgtc gcagttcact cggggcaaga
     6241 gctttcagga ttatcggaac gatgcaatgc tccgtctcgc agtggagcgc gcattctcga
     6301 tcatcgggga agcccttgtc caactggctc gaattgatcc gccgagtgcc ggcaggatca
     6361 cagatcatca gcgcatcatt gcgttccgca acattctggt tcatgcgtac gcgcacgttg
     6421 acaatcggct cgtatgggag atcgtcgaat acaaactgcc tggactgatg aacgaagtca
     6481 gcgaattgat gaatgatcct gacgagcctt cgtagcgagg tgctcaccgt tgcagtcctc
     6541 aaacgcaacg gcgagcattt cgcttgaaat gcctaccagc caaaaccacg atcgtcgcga
     6601 cgatcgaagt cctggaactg ctggaggacg agcctggagc cgcccagatc cgtatcggtg
     6661 aatccgaaga cgaagaactg gttcgggttg gggatctgat tgccattggc gtcggcaacc
     6721 gtttcgagga agtcgttgtc gttggcgacc cagagggtgt ggaggcgggt gccactgctc
     6781 cagatgtctg agccaaaggt gatgccttcg atcttggcgg gaatgttgcc aggatcgatg
     6841 ccgatcgccg tcagcgattt cacgatatcg aggaaaaggg ctttgggaac tgtgttcgcg
     6901 gccgccgtgg ttccatccat gtcgcggatg tcggtggcgc cgttgaggtc gatcttgaac
     6961 agctgtttga ccttggcctt actgccatcg gcacggccct tgccgtcgcg ctcgtcgacc
     7021 aggaattcgt gattgttgag cgccagaatt tcgctgacgc cgctgcccga tgtaagcagg
     7081 tatccgtact cgtgtgtcat acggcccgat gcgatgtcga tgacggcgat gcggagcagg
     7141 tttttggcgg
//
LOCUS       DASZYN010000389         3288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_530004, whole genome shotgun sequence.
ACCESSION   DASZYN010000389 DASZYN010000000
VERSION     DASZYN010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530004"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VHE33_09950"
     CDS             <1..280
                     /locus_tag="VHE33_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97820.1"
                     /translation="ETPPMKKGSSTDPFPIVYPGHPAMQAVSPAFVTLVPGFSTIFLR
                     QTADRGNLPDHNLCLNFHGLFCFLLLGSLFSAFSTFTIGFRLRRLGLP"
     gene            299..766
                     /locus_tag="VHE33_09955"
     CDS             299..766
                     /locus_tag="VHE33_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT97821.1"
                     /translation="MPPSMPAQSAPQRVYIVDDEWIIAETLAAILNHNGFSAHAFHSP
                     NRALVRALDQPPDILLTDVMMPDLSGIDLAINIRRANKNCRILLFSGQAGALDLLCDA
                     RRRGYEFELLQKPLHPMQLLLRLRNSRRRSKPPSDAESLPGDAQSNPGTSPAA"
     gene            complement(823..1203)
                     /locus_tag="VHE33_09960"
     CDS             complement(823..1203)
                     /locus_tag="VHE33_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97822.1"
                     /translation="MESNAVRRELHFVAWIKAQRGIHRELVQRALIEPVVESMPVAAG
                     TGSGGEQLQREGKKDWASGVPDGPIVGAAVLGACIGCPIGVAVDGALGGFLAGFIGAL
                     LGEVTVLGVAKLMKLALRAWRWAH"
     gene            1496..2824
                     /locus_tag="VHE33_09965"
     CDS             1496..2824
                     /locus_tag="VHE33_09965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HVT97823.1"
                     /translation="MKLNPLLLRSTLVGALGGLLFGFDTAVIAGTTHQLTEVFHLSAG
                     ALGLTVSIALWGTVAGAIGAGPLGQKIGGREALRIMALLYLISSLGCALAWNWPSLIA
                     FRFIGGLGIGGSSVLGPVYLAELAPARLRGRLVGLFQINIVAGILLAYASNYAVARLI
                     DGGDAWRWQFGVATIPAALFALFLFGIPRSSRWLVTQNRIDEARKVLQMMGTPDTEAE
                     LKEIIDSIHVERISQAEPLFQRKYRLPIFLAITIGMFNQLAGINAILYYANDIFGYAG
                     FSSISGDKQAVLLGAMNFCATILAMTVIDKIGRKSLLLIGSVGTAACLAGVAGIFASG
                     SHQGDLVWLLVGFIFFFSISQGAVIWVYISEVFPTRVRSKGQSLGSSSHWIMNAFISG
                     LFPWVAAHSKAAPFGFFAAMMVLQFFVVLFMYPETKGVSLEQLQHKLGID"
     gene            complement(2906..>3288)
                     /locus_tag="VHE33_09970"
     CDS             complement(2906..>3288)
                     /locus_tag="VHE33_09970"
                     /inference="COORDINATES: protein motif:HMM:NF014049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HVT97824.1"
                     /translation="TVMTAAMAEACADTLSSELGQVLGGTPRMITSLRRVPAGTDGAI
                     SLAGTLAGAIGAAVVVGVALLALPQLSRVDAAVALGAGIAGTVVDSLLGAGPERQGWL
                     NNDAVNALSTLTAAALAAWMVGRV"
BASE COUNT          568 a         1193 c          831 g          696 t
ORIGIN      
        1 ggagacgccc ccaatgaaaa agggatcgtc taccgatccc tttccaatag tttacccggg
       61 gcatccggcg atgcaagcgg tttctccggc cttcgttacc ttagtaccgg ggttttccac
      121 catctttctg cgccaaaccg cggacagggg caaccttcca gaccataatt tatgtctaaa
      181 tttccacgga ttgttttgtt ttttgctgct gggttcgttg ttctctgcgt tctccacttt
      241 taccataggt ttccggcttc gccgtctcgg tcttccctga tttgtctcgg gagtcatcgt
      301 gccgccatcg atgcccgccc aatccgcacc gcagcgcgtc tacatcgtgg acgatgagtg
      361 gattatcgct gagacgctcg ccgccattct caatcacaac ggctttagcg cacacgcctt
      421 tcacagtccg aaccgcgccc ttgtgcgtgc gctggatcag cctcctgaca tcctgctaac
      481 cgacgtgatg atgcccgacc tgagcggcat cgacctcgcc atcaacattc gccgcgctaa
      541 caagaactgc cgcatcctgc tcttctctgg ccaggcgggc gctctcgatc tgctgtgcga
      601 tgcccgtcgt cgcggctacg agttcgagct gctccagaag ccgcttcacc ctatgcagct
      661 tcttctccgc ctgcgcaact cccggcgccg cagcaagcct ccctccgacg cggagtccct
      721 ccccggagat gctcagtcca acccgggcac gtcgcccgcc gcctgattcc ggctcgcctt
      781 ctcagcgaac cggcagaacc tcgatccaaa agacgacctg cgttagtgcg cccagcgcca
      841 tgcgcgcagc gccagcttca tcagtttcgc gacccccagg acggtcacct ctcccagcaa
      901 cgctcctatg aacccggcga gaaagccccc caacgcgcca tccacggcca caccgatcgg
      961 acacccaatg catgccccca gcaccgcggc cccgacgatg ggcccatcgg gcacaccgct
     1021 cgcccagtct ttcttccctt cgcgctgaag ctgctcgccg ccgctgccgg taccggccgc
     1081 gactggcata ctctccacca ccggctcgat caaagccctc tgaaccagtt ctcgatggat
     1141 ccctctctgc gccttaatcc aggcgacaaa gtgaagttcg cgccgcactg cattgctttc
     1201 catgcatgtc tcctgaagcg ccaactttat gcgccgattt cagccatcag gggagtttcg
     1261 cgcggccaca agcttcgtag atccgcgcct tgtgaaacgc gattctaccc agcctgtgac
     1321 ggcgccacca tgccaattcc gtaatgccgg acgcatgact cgtaacacgc ccgtctcctt
     1381 ccacctccct gcccccggct tgcggggcat ctgacagagg gaatccgtaa ccattcggtg
     1441 attcctgcac gctgtacctg catgctgtgg cgtgcacgtg taccctgtac cctgcatgaa
     1501 actgaacccc cttctgctcc gcagtacgct ggttggcgcc ctcggtggac tgctcttcgg
     1561 cttcgacacc gccgtcatcg ccggcaccac ccaccagctc accgaagtct tccatctctc
     1621 cgccggagct ctcggcctca ccgtctctat cgctctctgg gggactgttg ccggagccat
     1681 cggagccggc cctcttggcc agaagattgg aggccgcgaa gctctccgca ttatggcgct
     1741 cctctacctc atttcatcgc tcggctgcgc gctcgcctgg aactggcctt ccctgatcgc
     1801 cttccgcttc atcggtggac tgggcatcgg tggctcctcg gtcctcggcc ccgtttatct
     1861 cgccgaactc gccccggcgc gcctgcgcgg gcgcctcgtc ggtctcttcc agatcaacat
     1921 cgtcgccggc atcctgctcg cctacgcctc gaattacgcg gtcgcacgtc tgatcgacgg
     1981 tggcgacgcg tggcgctggc aattcggcgt cgctaccatt ccagccgccc tcttcgccct
     2041 ctttctcttc ggaatcccac gcagttcacg atggctcgtg acgcaaaacc gcatcgacga
     2101 agcccggaag gtgctgcaga tgatgggcac gcccgatacc gaggcagaac tcaaagaaat
     2161 catcgactcg atccacgtcg agcgcatctc ccaggccgag ccgctcttcc aacggaagta
     2221 tcgcctgccg atcttcctcg ccatcaccat cggcatgttc aatcagctcg ccggcatcaa
     2281 tgccatcctt tattacgcca acgatatctt cggttacgcc ggcttctctt caatctcggg
     2341 cgacaaacag gccgttcttc tcggcgccat gaatttctgc gcgaccatcc tggccatgac
     2401 cgtcatcgac aaaatcggcc gcaaatcgct gctgctcatc ggatcggtcg gcactgcggc
     2461 ctgccttgcc ggagtcgccg gcatcttcgc ctccggctcc caccagggcg accttgtctg
     2521 gctgctggtc ggcttcatct tcttcttctc gatctcacag ggtgcggtca tctgggttta
     2581 catctcggaa gtcttcccca cgcgcgtccg ttccaaaggg cagagccttg gttccagctc
     2641 gcactggatc atgaacgcct tcatctcggg actgttcccc tgggttgcag cccattccaa
     2701 agccgcgcca tttggattct ttgccgcgat gatggtcctc cagttctttg tggtgctgtt
     2761 tatgtatccg gagacgaaag gcgtcagcct cgaacagctc cagcacaaac tggggatcga
     2821 ctagcttcga agcataaccc tgatggtgcc ccgtatccct ggtgggtgag ttgtcagacg
     2881 gatcgccctt tctgttggga gggaatcaaa ctctcccaac catccaggcg gcaagcgcgg
     2941 cagccgtgag cgtcgacagc gcattcacgg catcgttatt caaccacccc tggcgctccg
     3001 gcccggcgcc caggagggag tcgaccaccg tcccggcgat ccccgcgccc aacgcaaccg
     3061 ctgcatcgac ccgcgacaac tgcggcagag ccagcaacgc gacccccacc acgactgcag
     3121 ccccgatcgc accggccagc gtgcccgcca gggatatcgc tccatcggta cccgctggaa
     3181 cacgccgcag acttgtgatc atccgcggcg ttccacccag cacctggccc agctccgacg
     3241 aaagcgtatc tgcacaggct tcagccatcg ctgccgtcat gaccgtca
//
LOCUS       DASZYN010000390         4441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_917195, whole genome shotgun sequence.
ACCESSION   DASZYN010000390 DASZYN010000000
VERSION     DASZYN010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917195"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VHE33_09975"
     CDS             complement(<1..494)
                     /locus_tag="VHE33_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HVT97825.1"
                     /translation="MPVPFTRSNALRQRAEKLFPGGVNSPVRAFRAVGGEPPFVARGE
                     GASLWDADGNRYIDYFGSWGPMILGHAFPPVVEAIQKAAANSASFGASTGAEADLAEK
                     VIACYPAIEKLRFVSSGTEATMSAIRLARAATGRKIIVKFEGCYHGHADGLLVKAGSG
                     VATFG"
     gene            558..2315
                     /locus_tag="VHE33_09980"
     CDS             558..2315
                     /locus_tag="VHE33_09980"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HVT97826.1"
                     /translation="MKNWPASLLTCAALIMTTAAVNPEADPGVQFRTLADQYFSDVMF
                     RFGPTTATLAGFHQYDTKLEDYSRAGVDAEIAALREFEWKFDDFPETALDENTKADLA
                     IMRSQVRSNLLELETVRSWQKNPDTYSSGISNSAFALMERDFAPADQRLKSLIAREKQ
                     MPAVFAEARRNLEDPPRIYTEIALEQLPGIIDFFRHDVPEAFKAAKDPATIAEFQNTN
                     AQVIAALQSYEQWLKTDLLPKSNGDFRIGADTFRKKLLYDDMVDTPLDRLLQIGYDDL
                     HRNQAEFQRVAKEIDPTKTPKQVLAELADLHPAPDQLMQSFHNTFNSLIAFINAKRII
                     TIPSTVQPTLEETPPFMRATTQASMDTPGPYEKHSTTAYFNVTLPEKGWPASRISEYM
                     AAFNRGTIVSTSVHEAYPGHYVQFLWVNHGNLSAVRKLTGSATNVEGWAHYCEQMMLD
                     EGYNQPGVGAKNERESQMIRLGQLQDALLRNARFIVGIRMHTQDMGLKEAEDFFVNEG
                     YQSRPIAEVETKRGTSDPTYLYYTLGKLEIMKLRSDLQRKQGANFSLEKFHDAFMRQG
                     AAPIKIVRKALLGDDSPTL"
     gene            2416..3285
                     /gene="galU"
                     /locus_tag="VHE33_09985"
     CDS             2416..3285
                     /gene="galU"
                     /locus_tag="VHE33_09985"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HVT97827.1"
                     /translation="MKVRKAIFPAAGFGTRFLPATKSIPKEMLALVDKPIIQYGVEEA
                     LAAGCDQIVIVTGRGKSAIEDHFDVSYELECTLEKRGKKDLLAVSRQVSHMVRVSYVR
                     QKEALGLGHAVLMARDLVGDEPFAVILPDDVIDAPVPCLKQMMDVFEKTQSSIVATQV
                     VEGPAISAYGVLSGKQVNGNSRLFDVSGLVEKPKLADAPSKNAIIGRYILTPKIFDLL
                     EHTPLGAGGELQLTDGIKGLLETEKVYGYSFEGTRHDAGDKFGMLKATVDFALKREDL
                     GPAFRSYLKSLKL"
     gene            3542..4441
                     /gene="panB"
                     /locus_tag="VHE33_09990"
     CDS             3542..4441
                     /gene="panB"
                     /locus_tag="VHE33_09990"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HVT97828.1"
                     /translation="MSVTQFRTQDGSAPPPVPVTFQALQAKKQTGTPISALTAYDYAT
                     ARLVDEAGIDLILVGDSLAQVVLGYDNTLPVTVDEMLHHTRAVRRAVKRAIVAADMPW
                     GSYHLTIDDGVKNAVRFVKEAGAQAVKIEGGSSRVELVERLTSAEIPVIGHLGLTPQS
                     VHRMGGYKVQGKSIAAINELLHDAHALESAGAVLLVLEGVPREVAARITVEVGIPTIG
                     IGAGPDCDGQILVFHDLVNLSFSPPAKFVRQYGDAAALFRTAIEGYRQDVEGRAFPAD
                     NESYHLPKDVAAAFDGSPLSRKA"
BASE COUNT          957 a         1492 c         1198 g          794 t
ORIGIN      
        1 ccaaacgtcg ccacaccgga acccgccttc accagcaaac catcggcatg tccgtgatag
       61 cagccttcaa atttgacgat gatcttccgg cccgtcgccg cccgcgccag tcggatcgcc
      121 gacatcgtcg cctcagtccc cgagctgacg aagcgcagct tctcaatcgc gggatagcac
      181 gcgattacct tctccgccag atcagcctcg gcccccgtgg acgccccaaa actggcactg
      241 ttcgcagccg ccttctgaat cgcctccacc accggcggaa aagcatggcc gaggatcatt
      301 ggcccccagg aaccgaaata gtcaatataa cgattgccat cggcatccca cagagatgcg
      361 ccctcgcccc gcgcgacaaa cggcggctcg ccgcccacag cgcgaaacgc gcgtacgggc
      421 gaattcactc cgccgggaaa caatttttcg gcgcgctgcc gcagcgcatt ggaccgggtg
      481 aaagggacgg gcatacttcc agtataggtt ctgacggatg gcaggctttc cgcagttatc
      541 cgatactaag atcaccaatg aagaactggc ccgcctctct gctgacctgc gctgctttga
      601 tcatgacgac cgccgccgtg aaccctgaag ccgatccggg cgtccagttt cgcacgctcg
      661 ccgatcaata tttctccgat gtgatgtttc gctttgggcc cacgactgcc accctggccg
      721 gttttcatca gtacgatacg aagctcgagg actatagccg cgcaggggtg gacgcggaga
      781 tcgccgcgct gcgggaattc gagtggaagt tcgatgactt ccccgaaacc gctctcgacg
      841 agaacacgaa ggccgacctc gcgatcatgc gcagtcaggt gcgcagcaac ctcctcgaat
      901 tggagaccgt ccgctcctgg caaaagaacc ccgataccta ctccagcggt atctccaaca
      961 gcgccttcgc cctcatggag cgcgacttcg ctccggcaga ccagcggctc aaatccctga
     1021 tcgccagaga aaagcagatg cccgccgtct tcgccgaggc gcgcagaaat ctcgaggacc
     1081 ctccccgcat ttacaccgag attgcgctcg aacaattgcc gggcatcatc gacttcttcc
     1141 gccacgacgt gcccgaagcc ttcaaagcgg ccaaagatcc agccacaatc gccgaattcc
     1201 agaacaccaa cgcccaggtg attgccgcgc tccaaagcta cgagcaatgg ctcaaaaccg
     1261 atctgttgcc aaagtcgaat ggcgattttc gcatcggcgc ggataccttc cgcaagaagc
     1321 tcctgtatga cgacatggtg gatacaccgc tcgatcgcct gctccagatc ggctacgacg
     1381 atctgcatcg caaccaggcg gagtttcagc gcgtggcgaa ggaaatcgac ccgacaaaaa
     1441 cgccgaagca agtcctcgcc gaactggcgg acctccaccc agcgccggac cagctgatgc
     1501 agagcttcca caatacgttc aacagcctga ttgccttcat taacgcgaag aggatcatca
     1561 ccatcccatc gacggtgcag cccaccctgg aagaaactcc gcccttcatg cgcgcgacca
     1621 cccaggcgtc gatggacacg ccgggccctt acgaaaaaca ttccaccacg gcatacttca
     1681 acgtcacgct gccggaaaaa ggctggccgg cgtcgcgcat cagcgaatac atggcggcgt
     1741 tcaatcgcgg caccatcgtc agcacgtcgg tgcatgaagc ctatcccggc cactacgtgc
     1801 agttcctgtg ggtcaatcac ggaaacctga gcgcagtgcg caagctcacc ggttccgcca
     1861 cgaacgttga aggatgggct cactactgcg aacagatgat gctggacgag ggctacaacc
     1921 agccgggtgt cggcgcaaag aacgagcgcg agtcgcagat gatccgactc ggccagcttc
     1981 aggatgccct gctgcggaat gcgcgattca ttgtgggcat tcgcatgcat acccaggaca
     2041 tgggcctcaa agaggcagag gacttcttcg tgaacgaagg ctaccagtcc cggcccatcg
     2101 ccgaggtgga aaccaaacgc ggcacctcag acccgacgta tctctactac acgctgggca
     2161 agctcgaaat catgaagttg cggtctgacc tccagcgcaa acagggtgcc aacttttcac
     2221 tcgagaagtt tcacgacgcc tttatgcgcc agggagccgc gcccatcaaa atcgtccgca
     2281 aggccctgct tggcgacgac tcgccaacat tgtgagcagc cgtcgcatcc tactgctttc
     2341 tcgcgataga atgcccggtt tgtgttacag gcctgtccgg cgaggacatc taacattaag
     2401 gaaatgccga aaccgatgaa agtacgcaag gcaatctttc cggcggcggg cttcggcacg
     2461 cgctttttgc cggccaccaa gtcgatcccc aaggaaatgc tggcgctggt cgacaagccc
     2521 atcatccaat acggcgtcga agaagccctc gccgctggct gcgatcagat cgtgatcgtc
     2581 accggccgtg gcaagtcggc catcgaagat cacttcgacg ttagctacga gctggagtgc
     2641 accctggaga agcgcggcaa gaaggacctg ctcgccgtta gccgccaggt ttcgcacatg
     2701 gttcgcgtct cctacgtgcg gcagaaagaa gccctcggtc tgggtcacgc cgttctgatg
     2761 gcccgcgacc tcgtcggcga cgaacccttt gctgtcatcc tgcccgatga cgtcattgac
     2821 gcccccgtgc cctgcctcaa gcagatgatg gacgtctttg agaaaacgca gtcctccatc
     2881 gtggccaccc aggtcgtcga aggtccggcg atctccgcct acggcgtcct cagcggcaaa
     2941 caggtcaacg gcaactcccg cctcttcgac gtctccggcc tcgtggagaa gcccaagctc
     3001 gcggacgcac cctcgaagaa cgccatcatc ggccgctaca tcctcacgcc aaaaatcttc
     3061 gatctcctcg aacacacacc tctgggtgcg ggaggcgaac tccagctcac cgatggcatc
     3121 aagggcctgc ttgaaaccga aaaagtctac gggtatagct ttgagggcac acgtcacgac
     3181 gccggcgaca agtttggcat gttgaaggcg accgtcgact tcgccctcaa acgcgaagac
     3241 cttggaccgg ccttccgcag ttacctcaaa tcgctgaagc tgtagctgca ggcgattttt
     3301 gtaaaaccgg ttcggccctg aaactatatc gccccacgat ccgtccacag gcaaagggcc
     3361 gggaggtgct gttcaaagct ctgcgtttcg ctctgctgct gaccccggcc tgtttctcgt
     3421 cccgccgcac tacagtcatc cgccaaaacc tcatttgtct tctcccgccc tgacggcgta
     3481 tcgtcactcc taggttcggg tggcatcctc tgcaagcggg aggagccgca ctggagacga
     3541 catgagcgta acgcagttcc gcacccagga cggatcggca cctccgccgg ttcctgtgac
     3601 tttccaggca ctccaggcaa aaaagcaaac gggcacgccc atctccgccc tcaccgccta
     3661 cgactacgcc actgcgcgcc tggtcgatga ggccggcatc gacctgatcc tcgtcggcga
     3721 ctcacttgcc caggtcgtcc tcggctacga caacacccta ccggtcacag tcgacgaaat
     3781 gctgcaccac acgcgcgccg ttcgtcgtgc ggtaaagcgc gccattgtcg ctgcggacat
     3841 gccctggggc tcctaccacc tcaccatcga tgacggcgtg aagaatgccg tccgcttcgt
     3901 gaaggaagcc ggagcgcagg cggtgaagat cgaaggtgga tccagccgcg tcgaactggt
     3961 cgagcgcctc acctccgccg aaattcccgt catcggccac ctcggcctca ctccccaatc
     4021 cgttcatcgc atgggcggat acaaagtgca gggcaaatcc atcgccgcca tcaacgaact
     4081 gctccatgac gcgcatgcgc tggagtccgc cggagcggtc ctgctcgtcc tggaaggcgt
     4141 gccacgcgaa gtggccgcgc gcattacggt cgaggtcggc attcccacga tcggcatcgg
     4201 cgccggtccc gactgcgacg gccaaatcct cgtcttccat gacctcgtga atctcagttt
     4261 ttcccctccg gcaaagttcg tgcgtcagta tggcgacgcc gccgccctgt tccgcaccgc
     4321 gatcgaaggg tatcgccagg acgtcgaggg ccgtgccttc cctgcagaca acgaaagcta
     4381 ccatctgccc aaagacgttg cggcggcgtt cgacggcagc ccgctaagcc gaaaggcctg
     4441 a
//
LOCUS       DASZYN010000391         2156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_272282, whole genome shotgun sequence.
ACCESSION   DASZYN010000391 DASZYN010000000
VERSION     DASZYN010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272282"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VHE33_09995"
     CDS             complement(<1..710)
                     /locus_tag="VHE33_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97829.1"
                     /translation="MNDAIIIRGARTHNLKNIDCEIPHGKLTVVSGVSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLERIEKPDVELIDGLAPAIAIRQKNSTRNPRSTVATA
                     TEIYDYMRLLWARCGVVHCTVCGGIVRRDTMDEIAADILALGEGTRLYALFPVQAQAT
                     TPAQPVSDTAAEAKAPRRTRKTAVKTAEKASGGLSDALRERLGDLRKRGFNRLYQNGN
                     IYEFSTPESLLEIDFALPV"
     gene            813..1361
                     /locus_tag="VHE33_10000"
     CDS             813..1361
                     /locus_tag="VHE33_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97830.1"
                     /translation="MHWTGALILLSLAIVCASLMLVFLYLTRAGRILQERIPDRPRRR
                     LFLASVSFFITFLGVRLLVSAIVHHVGPFGFVMVRGHHIHHLVWGILLLLLVGYAELA
                     EIGTGASPSSIFFSRLISIAFGIGAALTLDEFALWLNLSADAYWTRQGRESIDAVILF
                     GALLAIGAWGAPIWTRRTHHHA"
     gene            1465..2151
                     /locus_tag="VHE33_10005"
     CDS             1465..2151
                     /locus_tag="VHE33_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97831.1"
                     /translation="MAPVARLMTTPLVHRAVRMVVMHWTAAWILIAFLAASISVILIL
                     LHLTRAGAVLHERIPDRPRRRLFLAALGFVLTFFGVRILVLFILFHKGPFGWVVLGGR
                     HIHHLVWGIVLLLVTGYAMVAEIGTEATPRSLFWGRLIAIAYGVGAALTLDEYSMWLD
                     IKESTWTISGRESIDAVVFFGALLALGAWGAPLWTKRPRKRRRRNHNHDAHSSRSSNG
                     ANRNPAGNSE"
BASE COUNT          344 a          830 c          545 g          437 t
ORIGIN      
        1 accggcagcg caaagtcgat ctccagcagc gactccggcg tcgaaaactc gtaaatattc
       61 ccgttctgat agagccggtt gaacccccgc ttgcgcagat cgcccagccg ctcccgcagc
      121 gcgtccgaca aaccacccga cgccttctcg gcggtcttta ccgctgtttt tcgtgtccga
      181 cgcggcgcct tcgcctctgc ggccgtgtcc gacacaggct gcgccggcgt cgtcgcctgt
      241 gcctgcaccg ggaacagcgc gtacaaccgc gttccctccc ccagcgccaa aatgtctgcc
      301 gcaatctcgt ccatcgtgtc gcgtcggaca attccgccgc acaccgtgca gtgcaccacg
      361 ccgcagcgtg cccaaaggag ccgcatgtag tcgtagattt ccgtggccgt cgccaccgtc
      421 gatcgcggat tccgtgtcga gtttttctgc cgaatcgcaa tggcaggcgc cagcccgtcg
      481 atcagctcca catccggctt ctcaatgcgc tccagaaact gccgcgcata cgccgaaagc
      541 gactccacgt accgccgctg tccctcggca taaatcgtgt caaacgccag cgacgatttc
      601 cccgaccccg acaccccgga caccaccgtc agcttcccgt gcggaatctc gcagtcgatg
      661 tttttcagat tgtgggtccg cgcgccgcgg atgatgatcg catcgttcaa agtgtggtcc
      721 cggtagttcg aactaagccc tgaggaaaaa gtcccccagt ctgattatag gggcggcttc
      781 agcccatcac ccaggcgcga caatggagac ctatgcactg gaccggagcc ctgattcttc
      841 tgtcgctcgc catcgtctgc gccagcctca tgctggtctt cctctacctc acgcgcgccg
      901 gcagaattct ccaggagcgc atccccgacc gtccccgccg ccgcctcttc ctcgcctccg
      961 tcagcttctt catcaccttt ctcggcgtac ggctgctggt ctccgccatc gtccaccacg
     1021 tcggcccctt cggcttcgtc atggtccgcg gccaccacat ccaccatctc gtttggggca
     1081 ttctgctgtt gctcctcgtt ggatatgccg agttggccga gatcggcacc ggcgcctcac
     1141 cctcctccat cttcttcagt cgactcatct ccattgcgtt cggcatcggc gcggctctca
     1201 ccctcgacga attcgccctc tggctcaacc tcagcgccga cgcttactgg acacgccagg
     1261 gtcgcgaaag catcgacgcc gtcatcctct tcggcgccct gctcgccatt ggcgcctggg
     1321 gcgcgcccat ctggacccgc cgcacccacc accacgcctg aacgcatccc cgcaaccttg
     1381 agccctgagc tctgcgacct gagctctgag ccctgttcca gccccggcca aatgccgata
     1441 accacaggtg aggcgttgcc tgcgatggct cctgttgcac ggctgatgac tacccctctg
     1501 gtccaccgcg ctgtcagaat ggtggtcatg cactggacgg ctgcctggat cctcatcgcc
     1561 tttctggccg cctccatctc cgtcatcctc atcctgctgc acctgacccg tgccggcgcc
     1621 gtcctgcatg agcgcatccc cgatcgcccc cgccgccgcc tcttcctcgc cgcactcggc
     1681 ttcgtcctca ctttcttcgg cgtgcgcatc ctggtgctgt tcatcctctt ccacaaaggc
     1741 cccttcggat gggtcgtact cggcggccgc cacattcacc atctggtctg gggcatcgtc
     1801 ctgctgctcg tcaccggcta cgccatggtt gctgaaatcg gcacggaagc caccccgcgc
     1861 tccctcttct ggggccgtct catcgccatc gcctatggtg tcggcgccgc cctcaccctc
     1921 gacgagtact ccatgtggct cgacattaaa gagtccacct ggaccatctc cggccgcgaa
     1981 agcatcgacg ccgtcgtttt cttcggcgct ctgctggctc tcggcgcctg gggcgcaccg
     2041 ctctggacta aacgcccccg caaacgccgc agacgcaacc acaaccacga cgcgcactcg
     2101 tcccgctcct caaacggcgc caaccgtaac ccggctggca acagcgaata gaaacc
//
LOCUS       DASZYN010000392        12556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_19003, whole genome shotgun sequence.
ACCESSION   DASZYN010000392 DASZYN010000000
VERSION     DASZYN010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 12556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12556
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19003"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(347..1846)
                     /locus_tag="VHE33_10010"
     CDS             complement(347..1846)
                     /locus_tag="VHE33_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97832.1"
                     /translation="MQALDVLAWARDAAGYDPDPWQSWFLTQAMGTLPDSTWAATDVG
                     LIVSRQNGKGTILEIRELGGLFVLGEELIIHTAHEFKTAAEHFRRIKQALDDHPALRK
                     RVKKIAGSHGEEAVELFPQPTLIFGAGKKQVLRRVAGRLRFLARSKGAGLGFSCDCLV
                     YDEAMILTEDQVAASLPTMSARANPQIWYTGSAGREDSFQFSSIRSRIVRDSKDLVGA
                     EWSISPHNDGCPRDETRGRASNRFIVCTKHDDRDVPESWAKANPGYGYRISEKFTRDV
                     ELKGMPMAKFDRERLGVGEWPQPEAPWAVISEIAWQKLAADRDKVGFPVQPIVFAVDI
                     DEDGLGATISAAWDHASGKMVLEIPKDCSRQGSDWVMGRLKTLYAKWKPLAIVMPKSG
                     PAAALIADGQKVWRDRLVAVGTAEEAAAFAWFLQQVRSESLCHFGAEASPTLWHAVST
                     AATRVVGDGGKAWSRRDSETDITPVTSATLAAYILNRDRRNYDVLASVA"
     gene            complement(2373..3131)
                     /locus_tag="VHE33_10015"
     CDS             complement(2373..3131)
                     /locus_tag="VHE33_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97833.1"
                     /translation="MAAKRVSALIAINGSLKEEADKARDQIAAVARQADFMDGFEKTW
                     QPLTEPDNPHAPPKRQGDSKRVRVTAEEVLSQLQRVMTAKWDTSLTLDTGNVGSVADV
                     RVDGEVLLRNVPVGHLLYLARELDKIGEIVTAIPVLANDKEWTADGTPAGIHRTARPV
                     VQYSTDKEPYVLVLSPATAQHPQRDQVMTRDVQDGWYTTTAFSGALTFDRKARIIERV
                     AKLQAAVKEAREEANTRQVEEQHEGDVIFGYLLS"
     gene            complement(3423..3839)
                     /locus_tag="VHE33_10020"
     CDS             complement(3423..3839)
                     /locus_tag="VHE33_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97834.1"
                     /translation="MSWFTAKRDGKCSHCKTPFQAGDEIYAKGAGVYLGSGCGCGHLA
                     EDGPLAAGPRESAVLRDLSKLPPEAMDGLIAQNMIGMARQLDEGDVAPREVTNYTKEL
                     RLNLLTLQDMFPPAEQDDETEEKRQRRQHRREAGGF"
     gene            4193..5125
                     /locus_tag="VHE33_10025"
     CDS             4193..5125
                     /locus_tag="VHE33_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97835.1"
                     /translation="MTRTAGKYGRRAPKRAAALKLGKYLTGVVPAVPADEDYLAALHG
                     GWQMLGNDRAGDCVAVTWANVRRLVTTSLTDKPSYPDQDMVWKIYQTQNPQFNPNGGS
                     ITGPGSEYDNGMDIQSLLEYLTRTAGPDGVKALGFAQVDHTNPAEVKAAIALFGYVWT
                     GINVQDVNQTQFSEGQPWDWSYDGQTEGGHSVVTGGYGTPGSGALGGDERFITWAEET
                     SFTDSFWKDGVEECWVVIWPEHLADTDFLAGVDLAALAADYQEITGKPFPATVNPQPA
                     PVPQPAPAPANPPAPPAGMVKWFEEFLAWLKSLV"
     gene            complement(5253..5549)
                     /locus_tag="VHE33_10030"
     CDS             complement(5253..5549)
                     /locus_tag="VHE33_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97836.1"
                     /translation="MTEMQPPEIKSLDFSEFHPLDHSVWACDGFPGGRKRHLQYRWDF
                     QWRDRLRSWTLCKIGRHRPVESWGRSREDAEAGRPMRSLGWSCACCYKDTPPVS"
     gene            complement(5542..5817)
                     /locus_tag="VHE33_10035"
     CDS             complement(5542..5817)
                     /locus_tag="VHE33_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97837.1"
                     /translation="MSDSSEVPLDRGGHGGSWYAGGAAFPPTDGHGCSFCGAVGGGGH
                     GGFCPNWGRRFDWDGNEISEEELGKSVADRIRENVNYIKRKYGNPDD"
     gene            complement(5950..6105)
                     /locus_tag="VHE33_10040"
     CDS             complement(5950..6105)
                     /locus_tag="VHE33_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97838.1"
                     /translation="MPDNRLRELNRKYAQAKRAALRELAARHQEEYWALYRAKRIEQE
                     RSVHEQD"
     gene            complement(6095..6301)
                     /locus_tag="VHE33_10045"
     CDS             complement(6095..6301)
                     /locus_tag="VHE33_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97839.1"
                     /translation="MSNEPKTSIDDRIDLADTAIGGQFTLKVKANRVCMSCDDCEWTE
                     STGEMPLWEWVCDAREHWEREHAR"
     gene            6559..6936
                     /locus_tag="VHE33_10050"
     CDS             6559..6936
                     /locus_tag="VHE33_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97840.1"
                     /translation="MPQCSVRNCTDTTNYGFKKLLGKWWCSRHAIPVEQQLRKQADEL
                     DWYDGNIRLREQALRRNEQNRESEAAIALRLNTRRDDPRVKREMAAQNQRTGSLRYEL
                     ETLKRNRATAAQRHEHDNGRRFG"
     gene            8194..8460
                     /locus_tag="VHE33_10055"
     CDS             8194..8460
                     /locus_tag="VHE33_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97841.1"
                     /translation="MTQLLHMITTAVYGGYPLPVWREVGAWLNAYGVAMTAVTAWPVL
                     RFAAWALANESRYPFTAWADCPDAIRSMITDYRATRRTLTGRPR"
     gene            8548..9252
                     /locus_tag="VHE33_10060"
     CDS             8548..9252
                     /locus_tag="VHE33_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97842.1"
                     /translation="MSVATVATSAKPARTRKLRAVATGEQPAVTETPAPANPTPDTDA
                     LVKDARRYVKNISGNAVKLAGVLVDLYQVKPWNAEEITLKEYMAGLGIGAGTDLLTLP
                     VDARRAVVSQMFTLDANASIDDIALLTGAGKRTIARDKSDLGFANPNRVESHTGPRNT
                     DDTDDPDDTDGATSETTPAAPAAKAAMTPQTVMAWLDTQDDQTIADIILGLADDRAET
                     VTDILIGALNARAAAV"
     gene            9355..9621
                     /locus_tag="VHE33_10065"
     CDS             9355..9621
                     /locus_tag="VHE33_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97843.1"
                     /translation="MSDVITRAGSVTRVANVKTPHANLFTGLSAADVAAILGAGPLAM
                     RAMITAEACGRARFGNVATVYCEGYQRNAAHCDAGSAVYRVALS"
     gene            9618..10070
                     /locus_tag="VHE33_10070"
     CDS             9618..10070
                     /locus_tag="VHE33_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97844.1"
                     /translation="MSDLATLIDNLTVSLRPHAATPVTVRRLDRDSPAPAAPSGTGEI
                     VTGVTGRGDGRRRKRNTNANRRNGGGSCYYRPKADHYAGGPPATTVPNAPRMTPAKPM
                     PEDTAYRTVTSSRRLLVEASAEIPAPGTMYPNGTSRRGNPAIRTARTR"
     gene            10180..10482
                     /locus_tag="VHE33_10075"
     CDS             10180..10482
                     /locus_tag="VHE33_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97845.1"
                     /translation="MSRPAMRDWLDLAWWHALTVWHVAKAFAAETWESIPGPWPAKIL
                     VCVLLVACLAIPGPFDEMLVLALIKLARKRREKRAVVAAYGYQARYGNGQITRADS"
     gene            10524..10817
                     /locus_tag="VHE33_10080"
     CDS             10524..10817
                     /locus_tag="VHE33_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97846.1"
                     /translation="MFLLSPVERDLVRDIFRIFLDDPTGFKLTADLYSRGLSLDEVRG
                     YRSDMERLTARIDYADAPSPKHADTSVRPDQKPDGYQGRHAAPLDDFAWMAAR"
     gene            10931..12112
                     /locus_tag="VHE33_10085"
     CDS             10931..12112
                     /locus_tag="VHE33_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97847.1"
                     /translation="MANLPNLSDREVIIGGGAHAAVYAATRVKLGKRQPVVLEAEARC
                     GGVFTRLSRVPFWMNSASGASVDSTGAGPTRIPSRSVTDLLNWLPNCEVQADGPGSDN
                     QTEYPLSTMMGSAIRRSLARAAEVYTGVRAYVTDVARREITTQDGDSYRCGRIIDARG
                     LNMDSRRPELSMCKELTPVITAEMFLRGMWPSGISSRGPQRVALVGGGDTACVVAEAL
                     LGQGPEGWPFAAESIDWFAVDLPGSKSDWLDSKHARYAGLARHFPQAERRGMIRPRQQ
                     RVDTETVQVIGDAVRVGFDTYDLAVFCTGFQPAFAEYGPGLADNVYRPGVYRDERGLW
                     RRDGEYYIVGTAAGLAQQRGELDKGYSRFPKNTDALYRLAPETAKLAALLDDAPVSSG
                     Y"
     gene            12142..12384
                     /locus_tag="VHE33_10090"
     CDS             12142..12384
                     /locus_tag="VHE33_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97848.1"
                     /translation="MTNEVTEASAAEIIAWLRSPEGEAWSEKRLAMACYLAYGHYAPN
                     IAATFTRLEDRPVVMMRGMFSIENAPDDHPEEAEHE"
BASE COUNT         2247 a         4300 c         3977 g         2032 t
ORIGIN      
        1 tcatcaccga cgcccgcctc accccgccgc cgcccccggc gaaggaagac ccgttcatca
       61 cgaagctccg cgccgagaac gaccgcttcc ggcagctgtg ggcactgcag ccttagtgcc
      121 acgtggcagt aacgggccat aagcggagcg gaaacccatc gaaccagcgc ccgttactgc
      181 tacaactggc ctcgtggcac gtcacatctg catcttcagc aagttcgtta agaagtggaa
      241 agtgaacggc aagacctggg cgaagtacca gtgcagcaag tgcaccgccc aggagatcgt
      301 gtcgctgtag ggccaccgat aagacggagc ccatgaggaa agcgccctat gcgaccgagg
      361 caagaacgtc gtagttccgc cggtcccggt tgaggatata ggcggccagc gttgcgctcg
      421 tgacgggcgt gatgtcggtc tctgaatccc gccgggacca cgctttcccg ccgtccccca
      481 ctacccgggt cgccgccgtc gagaccgcat gccacagtgt cggggacgcc tccgcaccga
      541 agtggcacag cgactcactt cgcacctgct ggaggaacca ggcgaacgcc gccgcctcct
      601 cggccgtgcc caccgcgacc aggcggtccc gccacacctt ctggccgtcc gcgatcagcg
      661 ccgccgccgg gccggacttg ggcatcacga tcgccagcgg cttccacttg gcgtacagcg
      721 tcttcagccg gcccatgacc cagtcgctgc cctggcggga gcagtccttc gggatctcca
      781 gcaccatctt gccgctggcg tggtcccagg cggccgagat cgtcgcgccc aagccgtctt
      841 cgtcaatgtc gaccgcgaac acgatcggct ggaccgggaa accaaccttg tcgcggtcgg
      901 ccgccagctt ctgccaggcg atctcgctga tgaccgccca gggcgcctcc ggctggggcc
      961 actccccgac gccgagccgc tcccggtcga acttggccat cggcatgccc ttgagttcga
     1021 cgtcccgggt gaacttctcc gagatgcggt agccgtagcc ggggttggcc ttcgcccagc
     1081 tctcaggcac gtcgcggtcg tcgtgcttgg tgcagacgat gaagcggttg gacgcccggc
     1141 cccgggtctc gtcgcggggg cagccgtcgt tgtgcgggct gattgaccac tcggccccca
     1201 ccaggtcctt gctgtcgcgg acgatgcgcg accggatcga ggagaactgg aacgagtcct
     1261 cgcggccggc cgagccggtg taccagatct gcgggtttgc ccgtgcggac atggttggca
     1321 gggacgccgc gacctggtcc tcggtcagga tcatcgcctc gtcgtagacc aggcagtcac
     1381 aggagaagcc caggccggcg cccttagacc gggccaggaa ccgcagccgc ccggccaccc
     1441 gccgcagcac ctgcttcttg cccgccccga agatgagcgt cggctggggg aacagctcga
     1501 ccgcctcctc accgtgggag cccgcgatct tcttcacccg cttgcgcagc gccgggtggt
     1561 cgtccagtgc ctgcttgatc cggcggaaat gctcggcggc ggtcttgaat tcgtgggcgg
     1621 tgtggatgat cagctcctcg cccagcacga acaggccgcc cagctcccgg atctccagga
     1681 tcgtgccctt gccgttctgc cgcgagacaa tcagcccgac gtcggtggcc gcccaggtgg
     1741 agtccggcag cgtgcccatc gcctgggtca ggaaccacga ctgccacggg tccgggtcgt
     1801 agcccgccgc gtcccgggcc caggccagca cgtccagcgc ctgcatgtcc ccgcagccgg
     1861 tcccgtccgg gtaacccgcg tcgttgagag agcacaccgg gcacgtccgg acggggcgct
     1921 catgccgggg cggcgcggtg aagaaccggg gccgctgggc accaaggagg acttcagggg
     1981 caacagtcat gcccacaggt taaagcggaa agtgccggaa aagcagggtg cccccctagt
     2041 cttgccctga ctaggggggc tttggactta cgggagtcga acccgtagac ccggattaga
     2101 agtccggtgc gtttgccgat tacgccaaag tcgctacttg taccctgcgg tgcgccgacg
     2161 gcccgtctga tggtggcatt cgctctgccg ctgagctaca ggcccacgtg gagggcccgc
     2221 cgggactcga acccgggaca atgccgttgc cgccgttgcg gagggtgatt ctgtgacgct
     2281 gagagtctga aggcttacta gtctgtgtct gatgcagagt ctggtccgca gtctgcgagt
     2341 ctgagcccta catcagacac actagcacac tttcagctca gcaggtaacc gaagataacg
     2401 tccccttcgt gctgctcctc gacctgccgg gtgttggcct cctcccgggc ctccttcacc
     2461 gccgcctgca gcttggcgac ccgctcgatg atccgggcct tgcggtcgaa cgtcagggca
     2521 ccggagaacg cggtcgtggt gtaccagccg tcctgcacgt cccgggtcat gacctggtca
     2581 cgctgcgggt gctgggccgt ggcgggcgac agcaccagga cgtacggctc cttgtcggtg
     2641 ctgtactgca cgaccggcct ggccgtccgg tgaatgccgg cgggggtgcc gtcggccgtc
     2701 cactccttgt cgttcgccag caccgggatg gccgtgacga tctcaccgat cttgtccagc
     2761 tcccgggcca ggtagagcag gtgcccgacc gggacattgc gcagcagcac ctcgccgtcg
     2821 accctgacgt ccgccaccga gccgacgttg ccggtgtcca gggtcagcga ggtgtcccac
     2881 ttggctgtca tgacccgctg cagctgggac agcacctcct cggccgtgac ccggacgcgc
     2941 ttggagtcac cctgccgctt gggcggggcg tgcgggttgt ccggctcggt cagcggctgc
     3001 caggtcttct caaagccgtc catgaagtcg gcctggcggg cgaccgccgc aatctggtcg
     3061 cgtgccttgt cggcctcttc cttcaggctg ccgttgatcg cgatcagcgc tgatacacgc
     3121 ttagcggcca tgttgtgtcc tcacttctcc ggggaacggg cagggaacgg tgtcgcacca
     3181 gccctcgtgc agggccagct caccgaatgc ccacttggtg ccgcagttgc atacgcgctc
     3241 acggtcatat gcccagctgg cggggatgcg gccggttccc tcgcacgggc agccggaaga
     3301 gtgcacgcgg ggacggcgcc gcaagaactg gatcgtcatg ttaactcctg gggatgtgcg
     3361 ttacagtcac accctacccc aggtgttgca tcacgtggta actgtttact gccacgtggc
     3421 agtcagaagc ccccggcctc acggcggtgc tgacggcgct gccgcttctc ctcggtctcg
     3481 tcgtcctgct cggcaggcgg gaacatgtcc tgcagcgtca gcaggttgag ccgcagctcc
     3541 ttggtgtagt tggtcacctc acgcggggcg acgtccccct catccagctg ccgggccatg
     3601 ccgatcatgt tctgggcgat cagcccgtcc atcgcctccg gcggcagctt ggacaggtcg
     3661 cgcagcaccg ctgactcccg ggggcctgcc gcgagagggc cgtcttccgc caggtggccg
     3721 cagccgcagc cggaccccag gtaaacgccg gcgcccttgg catagatctc gtcgccggcc
     3781 tggaacggcg tcttacagtg actgcacttg ccgtccctct tggcagtgaa ccagctcatg
     3841 acctgagcgt agctcccgca taaaggtgtt ccaggcatcc gccgtgaact ccggagtcac
     3901 gtcatcctga tgcgccccgt cagtcacaca agctcccaga atgtggtccg gccgccgtcc
     3961 ggaagctccc agctgtaacc gggcgtccgg gccggggagc tgtggatgcc gcaggcaatg
     4021 ccgttgccgt tggcgaacga cgcaccgtta gcgatagacg caccttgtga attactcttg
     4081 ctgttcgtag tcatacgcaa cactatagct cctgttcact tccccgcgtt agtctgtccc
     4141 ggtacccgcc gtggcctccg ggagccggca gtctcctact gaaggactgt cgatgacacg
     4201 taccgcaggc aagtacggcc gccgggcccc gaagcgcgct gccgccctga agctgggcaa
     4261 gtacctgacc ggggtcgtcc cggctgtccc ggcggacgaa gactacctgg ctgcgctgca
     4321 cggcggctgg cagatgctcg gcaacgaccg ggcgggcgac tgcgtcgctg tcacctgggc
     4381 gaacgtcagg cgcctggtca ccacctcgct gacagacaag ccgtcctacc cggaccagga
     4441 catggtctgg aagatctacc agacgcagaa cccgcagttc aaccccaacg gcggcagcat
     4501 caccgggccg ggttctgagt acgacaacgg gatggacatc cagtccctgc tggagtacct
     4561 gaccaggacg gccggccccg acggggtgaa ggcgctcggc ttcgcgcagg tcgaccacac
     4621 gaacccggcc gaggtgaagg ccgctattgc cctgttcggc tacgtctgga ccggcatcaa
     4681 cgtccaggac gtcaaccaga cccagttctc cgagggccag ccgtgggact ggagctacga
     4741 cgggcagacc gaaggcggcc actcggtcgt caccggcggc tacggcactc ccgggagcgg
     4801 cgcactgggc ggggacgagc ggttcatcac ctgggccgag gaaaccagct tcactgacag
     4861 cttctggaag gacggcgtcg aggaatgctg ggtcgttatc tggcccgagc accttgccga
     4921 cactgatttc cttgccgggg ttgacctggc cgcgctcgct gcggattacc aggaaattac
     4981 cggcaagccg ttcccggcaa ctgtaaatcc tcagccggca cccgtcccgc agcccgcacc
     5041 ggccccggca aaccccccgg ctcccccggc cggcatggtg aagtggttcg aggagttcct
     5101 ggcctggctg aagagcctgg tctgaggcag gcacacgaag aggcgccgtt ccccgactgg
     5161 tgcggcgcct cttcactgtc tcgtgagctt tttcccgtgg cttgcggaga ctctgagaaa
     5221 ctacacgaat ccccgcagaa ccgccagtaa cttcaggaaa ccggcggagt gtccttgtaa
     5281 cagcacgcgc acgaccagcc cagtgacctc atcggccgtc ccgcttcggc gtcctcccgg
     5341 ctccgccccc acgactcaac cgggcggtga cggccgatct tgcacagcgt ccaggaccgc
     5401 agccggtcac gccactggaa gtcccagcgg tactgcaggt gccgcttccg gcccccggga
     5461 aagccgtcgc aggcccagac gctgtggtcc agcgggtgaa actccgagaa atcaagcgat
     5521 ttgatctccg gcggttgcat ttcagtcatc cgggttcccg tacttccttt ttatgtagtt
     5581 aacgttctcc ctgatcctgt ccgctacgga ttttcccagc tcttcctcgc tgatctcgtt
     5641 cccgtcccag tcgaaacgcc ttccccagtt cgggcagaag cccccgtggc cgccgcctcc
     5701 cacagcgccg cagaacgagc acccgtggcc gtccgtgggc gggaatgccg cacctccggc
     5761 gtaccagctg ccgccgtggc cgccccggtc gagaggaacc tcgctgctgt cgctcatgcc
     5821 tgcctctttt ccttgcagtg catgcagaca tgctctgggt gcggaagggc aatgtggcag
     5881 cggtgccagg tttcgtcagg cgatttcttg cagccgagcc acttgacgct gcagataccg
     5941 tgcctgcggt taatcctgct catgcacgct cctctcctgc tcgatgcgct tcgcgcggta
     6001 cagcgcccag tactcctcct ggtgccgggc ggccagttcc cgcagggcgg cgcgcttggc
     6061 ctgggcgtac ttccggttca gctcccggag ccggttatcg ggcatgttcc cgttcccagt
     6121 gctcgcgggc gtcgcagacc cactcccaca gcggcatctc gcccgtgctc tcggtccact
     6181 cgcagtcgtc gcaggacatg cagacgcggt tcgccttcac cttcagcgtg aactggccac
     6241 cgatcgcggt gtctgcgagg tcgatccggt catcgatgct cgtcttcggc tcgttactca
     6301 tctggcatct ttgtcttccc gctgcacggc ccgtcgccgc cgcagctagc acggtgcact
     6361 ttcagccagc agctgcggct gcacaggaag ctgtgacccg acggcgggtg ccgccaccac
     6421 tcgaaatcgt tctcccagtc ctggtcgtcc tcgtcccaga tccagtcgtc gtacacctca
     6481 ccgcactgcc cgcaagctac tgcgctggga tgtggcttcg taggcatacg caacactata
     6541 ccgcgttaga attcaaccat gcctcaatgc tccgtacgca actgcaccga cacgacgaac
     6601 tacggcttca agaagctgct cggcaagtgg tggtgctcac gccacgccat cccggtggaa
     6661 cagcagcttc gcaagcaggc ggacgaactc gactggtacg acggcaacat ccgtctccgg
     6721 gaacaagccc tgcgtcgtaa cgaacagaac cgggaaagcg aggcagctat cgcccttcgt
     6781 ctgaacacac gtcgagacga tccccgcgtg aagcgcgaga tggccgcaca gaaccagcgg
     6841 accggaagcc tccgctatga gctagagaca cttaaacgca accgcgcaac cgctgcccag
     6901 cgtcacgaac atgacaacgg aaggaggttc ggctagagga actggagtgc taactgtaaa
     6961 tgaacacgtt tggtggtttc gaaaattaat cgggtgattc atttaggggg cggggctttt
     7021 cgaaatcagg gagagaaatc cgacacccgg acgtccccgt tgcagtctgc ctaaaataga
     7081 cccccatcga tataggcact cctcgatggt cctgttggat ggtggagtgc ccaccgtggc
     7141 ggctgagacg tggcgggtgc ggccgtgcga ggacacgggg aatgcccgcg ccgttactgc
     7201 cacgtggcag caaacgatgc ggtgagaggc aagcgatgcg ttgcgttgca agcaatgtca
     7261 tgtgaggtat gcctgtatgg ccctgtaacc catccctgag acatgtgcat acccatcccc
     7321 gtactaggta ccgacctggc gcatgcctgc ctgtacgtag ctgtctgcac cgtacagacg
     7381 catacctgcg cagctagcac acggcacacg tacagactca gtcatataca gcgtacacgt
     7441 acagtgtaca tagctagtag tcgacactag tcgagtacag tctagcaaac cggacataaa
     7501 agtacatatc agtcaggttt ataacggaat ggtcacggta tcgttgcaga acggtctcag
     7561 gtatttgaca gcatgtcatc actacgtagt gtgtggattg ttctaccgca ccgcccgccg
     7621 cacacaccgc ccgcgcttag cagccgggca ccgcgcgccg gcccgaatct tgagaactgg
     7681 acaccggaga gttggccgat agcaaggcca cacgtggaac gcgcacccgt ggaatatgcg
     7741 cagtgatcgg cttagcgttt cccgctacgt gcaacggcaa gcgatgcacg tagccggata
     7801 ggccggttca aacatcctcg gataacgggt ccgttcccgt accgacaggc aagccgctaa
     7861 tgcgtcgctt ctgacaagcg ccggcgttct gagcattgct ccgccgatct cgcgcaaccc
     7921 ttcccagtga cggttaggcc gcgtgcctcc ggcgttccga gatgacagca ctacccggcc
     7981 gccagcgccg cctggcgcgg ccggccgaat gcactaccca agcaagtagc cggcggtaac
     8041 tcgccaaccg atatcgcggt ccgtcgcgtg acctggccta gcggtctagg ttgcacgcgg
     8101 cttaacgaga tcactcgcaa acgagtaggc aagcgctcac agcgcacgcc ggcgctactg
     8161 ccacacggca gtaagaaccg aaacggaaac ctcatgactc agctactcca catgatcact
     8221 actgccgttt acggcgggta cccgctgccc gtgtggcggg aagttggcgc gtggctcaat
     8281 gcctacggag tcgccatgac tgccgttacc gcgtggcccg tgctcagatt cgccgcatgg
     8341 gctcttgcga atgaaagccg ctacccgttc accgcatggg cagactgccc ggacgcaatc
     8401 cgcagcatga tcactgacta ccgcgccacg cgccgcacgc tgaccggccg cccgcgctaa
     8461 cccccgccat acgtcacccg tcccaggacc gcaaggtggc gactagggga accgttccgc
     8521 ctagcaacca accgaaaggc aagaaccatg tctgtcgcca ctgtcgccac cagcgccaag
     8581 ccagcccgta cccgcaagct gcgcgcggtc gccacgggtg agcagccggc cgttaccgaa
     8641 acgccggccc ccgccaaccc cacgccagac actgacgcgc tggtgaagga cgcgcgccgc
     8701 tacgtgaaga acatctccgg caacgcggtg aagctggccg gcgtcctggt cgacctgtac
     8761 caggtcaagc cgtggaacgc ggaagagatc acgttgaagg agtacatggc cggcctcggc
     8821 atcggcgcgg gtaccgacct gcttacgctg cccgtggacg cccggcgcgc cgtcgtgtcc
     8881 cagatgttca ctttggacgc gaacgcttcc atcgatgaca tcgcgctgct gaccggcgcg
     8941 ggcaagcgca ccatcgcccg tgacaagtcc gatcttgggt tcgccaaccc caaccgtgtc
     9001 gagtcgcaca ccggcccgcg caacacggac gacacggacg acccggacga cacggacggc
     9061 gcgaccagtg agaccacgcc ggccgccccc gccgccaagg cagcgatgac gccgcagacc
     9121 gtcatggcgt ggctggacac gcaggatgac cagacaatcg cggatatcat cctcggtctg
     9181 gccgacgacc gggcggagac ggtcactgac atcctgatcg gcgctctcaa cgcgcgggcc
     9241 gccgctgtct gatcacctgc acccgccccg cccgcacgtc accagccata cggccgcagg
     9301 gtggcgacta ggggaaccgt tccgctagca accgacaacc aaaggtcacc tgtcatgtcc
     9361 gatgtcatca cccgcgcggg cagcgtcacc cgcgttgcga acgtcaagac tccgcacgcg
     9421 aacctgttca ctggcctgag tgccgctgac gtggccgcca ttctcggagc cggcccgctg
     9481 gccatgcgcg ccatgatcac ggcggaggca tgcggccggg cacggttcgg caacgtcgcc
     9541 acggtctact gcgagggtta ccagcggaac gccgcacact gcgacgccgg ctctgctgtc
     9601 taccgcgtgg cgctgtcatg agcgacctag ccacactgat agacaacctg accgttagcc
     9661 tccgcccgca cgccgcaacc cccgtgaccg tgcggcggct ggaccgtgac tcacccgcgc
     9721 cggccgcgcc aagcggcacc ggcgagatcg tgaccggcgt aaccgggcgc ggtgacggac
     9781 gccggcgcaa gcgcaacacc aacgcgaacc ggcgcaacgg cggaggatcg tgctactacc
     9841 gcccgaaagc cgaccactac gccggcgggc cgcccgctac caccgtgccg aacgcgccgc
     9901 gcatgacgcc ggctaagccc atgccagaag acacggccta ccgcacggtg acatcatcac
     9961 gccggctgct ggtcgaggcg tcagccgaga tacccgcgcc cggcaccatg tacccgaacg
    10021 gcacgtcacg gcgcggcaac ccggcgatcc ggaccgcccg cacccgttaa tgccacgtgg
    10081 cagtaaggag accgcaatgc gcaagcgaat catcctgatc ctggccggcc tgatcatggc
    10141 ggccggcgtg acgttcctgc cgggctgcga ggtgacacgg tgagccgtcc ggccatgcgt
    10201 gactggcttg acctggcgtg gtggcacgcg ctgaccgtgt ggcacgtggc gaaagccttc
    10261 gctgccgaaa cgtgggaaag catccccggc ccgtggccgg ccaagatcct ggtctgcgtg
    10321 ctgctcgtgg cgtgcctggc catccccggc ccgttcgatg aaatgctcgt gctcgccctg
    10381 atcaagctgg cccgcaagcg gcgggaaaag cgggcggtcg tggccgccta cgggtaccag
    10441 gcgcgctacg ggaacggtca aataacgcgg gcagacagct aactgccacg cggcagtaag
    10501 caactcacga aacggagaca cgcatgttcc ttctcagtcc tgttgaacgc gacctggtcc
    10561 gcgacatctt ccggattttc ctggacgacc caaccggctt caagctgacg gccgacctgt
    10621 actcgcgcgg gctgtctctc gatgaggtgc gcggctaccg ctcggacatg gagcggctga
    10681 ccgcccgcat cgactacgcg gacgcgccga gccccaagca cgcggacacc agcgtccgcc
    10741 cggaccagaa gccggacggc taccaggggc ggcacgccgc gccactggac gacttcgcat
    10801 ggatggcggc gcgctgatga ccgcagaccg tcgccttact gccacgcggc agtaagacgg
    10861 cggcgtgccg tcagggcacg aggcagtaaa gcaaaggtcc acatcagccc cacgaaaggg
    10921 aaagatcaac atggcgaacc ttccgaacct cagcgaccgg gaagtcatca tcggcggcgg
    10981 cgcgcacgcg gcggtctacg cggccacccg ggtcaagctg ggcaagcggc agccggtcgt
    11041 gctcgaagcc gaggcccggt gcggcggcgt gttcacgcgg ctgagccggg tgccgttctg
    11101 gatgaacagc gcttcgggcg cgtcggtcga ctcgaccggg gccggcccta cccgcatccc
    11161 gtcccgcagc gtgaccgacc tgctcaactg gctgcccaac tgcgaggtgc aggcagacgg
    11221 ccccggcagc gacaaccaga ccgagtaccc gctgtcgacc atgatgggct cggctatccg
    11281 gcggagcctg gcgcgggccg ccgaggtcta caccggcgtg cgggcgtacg tgacggacgt
    11341 ggcccggcgc gagatcacga cccaggacgg ggacagctac cgctgcggcc ggatcatcga
    11401 cgcgcgcggg ctgaacatgg acagccggcg gccggagctg agcatgtgca aggagctgac
    11461 ccccgtgatc acggcggaga tgttcctgcg cggcatgtgg ccgtcgggga tcagctcgcg
    11521 cggcccgcag cgggtggcgc tggtcggcgg cggcgacacc gcgtgcgtgg tggcggaggc
    11581 gctgctgggc caggggccgg agggctggcc gttcgccgcc gagagcatcg actggttcgc
    11641 cgttgacctg cccggcagca agagcgactg gctggacagc aagcacgcgc ggtacgccgg
    11701 gctggcgcgg cacttcccgc aggccgagcg gcgcggcatg atccggcccc ggcagcagcg
    11761 ggtggacacg gagaccgtgc aggtgatcgg ggacgccgtg cgcgtcgggt tcgacaccta
    11821 cgacctggcc gtgttctgca ccgggttcca gcccgcgttc gcggagtacg gccccggcct
    11881 ggccgacaac gtttaccggc cgggtgtcta ccgcgatgag cgggggctgt ggcggcgcga
    11941 cggcgagtac tacatcgtcg ggacggcggc cggcctggcg cagcagcgcg gggaactgga
    12001 caagggctac agccggttcc cgaagaacac cgacgcgctg taccggctgg ccccggagac
    12061 cgcgaagctg gcggcgctgc tggacgacgc cccggtcagc agcggctact gactgccacg
    12121 tggcagtaag gaaggacccg catgacaaac gaagtcacag aggcgagcgc agccgagatc
    12181 atcgcatggc tgcgctcgcc ggagggcgaa gcctggtccg agaagcggct ggcgatggcg
    12241 tgttacctgg cttacggcca ctacgccccg aacatcgccg cgaccttcac ccgcctcgaa
    12301 gaccgcccag tcgtcatgat gcgcggcatg ttcagcatcg agaacgcacc ggacgaccac
    12361 cctgaggagg cagaacatga atgacctgga ccggcagcgc cggcagtgca ctaaaggctg
    12421 actgccacgt ggcagtaacg agtgagaaag gagcaaggca atggcaaagc tgaagctgac
    12481 ctggcagcag gacggttacc gcaccgagta cataactgcg acctacacgc ggacagtcga
    12541 cacaaacgac tacaca
//
LOCUS       DASZYN010000393         7164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_146972, whole genome shotgun sequence.
ACCESSION   DASZYN010000393 DASZYN010000000
VERSION     DASZYN010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7164
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146972"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VHE33_10095"
     CDS             <1..622
                     /locus_tag="VHE33_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97849.1"
                     /translation="VHGYSDQAATDAMIKKVTDMTGLDPTFVKESGGRLETRAYLREV
                     HREQAKLGSVYDSNVTMFDPFPYSAEQEANDPLLATLIAPTTTAMVDWVTHTVGWKVD
                     ARYNALSYEVGSHWSRDEEMRRGSVTQLRQAVAADPKLRVMIVHGWNDLSCPFMGSIL
                     TVNEMPIMGDPTRVSVHEFPGGHMFYTREASRVALRKDVEAMYSKH"
     gene            complement(979..1485)
                     /locus_tag="VHE33_10100"
     CDS             complement(979..1485)
                     /locus_tag="VHE33_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97850.1"
                     /translation="MLFASDAVHPYGTFWLDVADKAIKTMAVFIGAAWTYNNVRRSRV
                     YARKLEPSVSGTIFEKNGGLYILISCQLKNIGQTKYAIEQKGTYVGAFTITDQGEERS
                     VFAMEIFKDHDWIEPGEQISEPLIGPIPDPKTYIALKLVLRIVSGGEEWNVSTIVRTD
                     THADNNNA"
     gene            complement(1635..2105)
                     /locus_tag="VHE33_10105"
     CDS             complement(1635..2105)
                     /locus_tag="VHE33_10105"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HVT97851.1"
                     /translation="MQLASGAESLTCAYCGNIVFPEKDDAGVRVLSEPGDELCPTCHT
                     GLVEAAFAGARLRYCIRCRGMLIDMDLFADLVQTLRRKNDGGGVIPNPPDRAELERHL
                     TCPHCRQAMETHFYAGPGNVILSDCERCSLDWLDHARLMRIAHAPDALREEAQA"
     gene            complement(2252..3232)
                     /locus_tag="VHE33_10110"
     CDS             complement(2252..3232)
                     /locus_tag="VHE33_10110"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HVT97852.1"
                     /translation="MLSLRQFLTSQLIDVIQWNEPEDGPLVWRYPMANAEIQNGAQLT
                     VRESQLAAFVNEGRIADVFGPGLHTLSTRNLPLLTDLMHWNKGFQSPFKSDVYFFSTR
                     LQIDQMWGTPTPITLREKEFGAVRLRGYGIYAYRIKDPRMFFTEVSGTRDRYFVSDLE
                     GQLRQTIVARMTEVFASSDVSFLDMAKNQAALSQKIADAVQPSFTELGLELTSFVVEN
                     LSLPDELTKVMDQRIGVQMAGNLTPYTQFQAAQALGAAAANPNGTAGAGVGIGAGITL
                     GRALAGSITATEITNSASASAIAALQKFCVSCGKPMVRTAKFCPECGLQQ"
     gene            3453..4640
                     /locus_tag="VHE33_10115"
     CDS             3453..4640
                     /locus_tag="VHE33_10115"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVT97853.1"
                     /translation="MLTYAIGFVQDVQLVCFAIVLTSMAIQDRENESLSWLAGGYVVG
                     ACGSLLDLGGRWLPHWMSMGLFMEAAPIGYACIYTSVAVFLRRGIKGRWLWLLIALGA
                     LPFFLRWSATQNLSASATLVDGILAVQTAATSLLLLSTTDRETRWPRRTLSAFLAVYS
                     AIEVSRVVIYLVTGKMPADVAPAAEIASGIVYVVSCSVLPLGFIWMMNARLLLHLNRQ
                     SMVDPLTELLNRRGLQAATDVELSRYERTRRDLAVVVLDLDFFKLLNDRYGHPGGDAI
                     LCAFSVFLRSMIRETDIAGRVGGEEFVVILPGTDAETAYSTIERLRHAVAEHTFELGT
                     RLIHITASFGITVSAGRSGLTWEKLQHEADLALYAAKAAGRNRSCLYDESLATAGPTL
                     VEN"
     gene            4731..5960
                     /locus_tag="VHE33_10120"
     CDS             4731..5960
                     /locus_tag="VHE33_10120"
                     /inference="COORDINATES: protein motif:HMM:NF017019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HVT97854.1"
                     /translation="MRFALIAVLCAMPLFGQSTSVPSKPKVRAITGFVRLDRSTWQQQ
                     IADTLSVLRKAKSEFEARGYAVESVRITTQPLAELVSGETEEQALAILGELDRLSVKE
                     EFLPDVGPAMLHDGDDPAPMHLLERALSTLPNIEASTIIADSDGIHWKTIRQTAELVK
                     YVAEHSPHAQGTFNFTATAMLKPLGPFFPGSWHNGAGQQFAIGFEGANVVADVFTKDK
                     GNYDAAVADLTRALSEHAQVADSIGRKVAAETGWNYVGVDPTPAPLADVSIGTAIEDY
                     TGARFGSSGTMTAARIITSAVKAVPVQQTGYSGLMVPIMEDKVLAQRWAESAYSIDSL
                     LAYSAVCGTGLDTIPLPGDVSIQQMERIYGDVASLATKWNKPLSARLQPVPGKKAGDR
                     TEFQDPYLFNTMIHPLP"
     gene            complement(6028..>7164)
                     /locus_tag="VHE33_10125"
     CDS             complement(6028..>7164)
                     /locus_tag="VHE33_10125"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HVT97855.1"
                     /translation="VFMLDEVDKLGRDFRGDPSSALLEILDPEQNNTFRDNYLDQAFD
                     LSKVLFICTANMLDTVPEPLRDRMEIIELQGYTEEEKKHIAFRYLVPRQIKENGIAPE
                     NIEFPEDSIGYIVRHYTREAGVRKLEQQIGTICRKQARRIAEGNTEKLVITKEVIQEF
                     LGGIKVRVDTEIAERTKRAGVAVGLAWTPAGGDVLFIEANRMKGKGGFTMTGQIGEVM
                     QESMQAALTWVRSNAKSLGLEEDLLKDTDLHIHVPAGAIPKDGPSAGVTMATALVSLL
                     TDTPVHPLTAMTGEITLSGNVLPVGGIKEKFLAAKRAGVKTVILPAENRLNVEEDLMP
                     EQIEGVEIRYASRIEDVLAVALPRSTAEEHRDEQVREEVLQHMA"
BASE COUNT         1325 a         1952 c         2392 g         1495 t
ORIGIN      
        1 cgttcacgga tacagcgatc aggctgcgac cgacgcgatg atcaaaaagg tcaccgacat
       61 gacggggctc gatccgacgt tcgtgaagga atccggcggg cggctcgaaa cccgcgcgta
      121 cctgcgcgag gttcaccgcg agcaggccaa gctcggttcg gtatacgact ccaacgtgac
      181 catgttcgat ccgttcccgt attcagccga gcaggaagcg aacgatccgc tgctggcgac
      241 gctgatcgcg ccgacgacga cagcgatggt cgactgggtc acgcatacgg tgggctggaa
      301 ggtggatgct cgctacaacg cactgtccta cgaggtcggg tcgcactgga gccgggacga
      361 ggagatgcgc cgtggttcgg tgacgcagtt acggcaggcg gtggcggcgg atccgaagct
      421 gcgggtgatg atcgtgcacg gatggaacga tctttcgtgc ccgttcatgg gatcgattct
      481 gacggtgaac gagatgccga tcatgggcga tccaacgcgc gtgtcggttc acgagtttcc
      541 cggcggccac atgttctaca cgcgggaagc gagtcgcgtg gcactgcgga aggacgtgga
      601 ggcgatgtac agcaagcact gagacacgca gcgaacggat caccaaaagc gagtgagcag
      661 gggagtgggg cgatccgctc cccttcttgt ccctgagggc aactggctat gcggggcgca
      721 atcaaatgct ggacggcgtt cagaagagtt atgaaaccat cgcgactgac ggcagacgag
      781 tagctgcccg ctcgtggatg ggactggccc gcaatttccc gctacggctt gtttggtttc
      841 actgacgcgc ttgccgttcg ttcgatgtcc ggaaactgcc gcttgttctt tccccacggc
      901 gagatcgctt tagccttttc gacttttgca gtcattttct tacttacctt tttgacaggc
      961 atcttttttc tccattacct aagcgttgtt attgtctgca tgggtgtccg tcctcacgat
     1021 ggtactcaca ttccattcct cacctccgga tacgattctt agtacaagct tgagagcgat
     1081 ataggttttt ggatcgggta tagggccgat taggggttcg gaaatctgtt cgccaggctc
     1141 aatccagtca tgatctttga agatctccat tgcgaatacg ctgcgttcct cgccctgatc
     1201 ggtaatcgta aaggctccca cgtaggtgcc tttctgctcg attgcgtatt tcgtctggcc
     1261 tatgttcttt agttgacagg aaatcagaat gtaaagcccg ccgttcttct cgaagatcgt
     1321 gccggaaaca gaaggttcaa gctttcgcgc gtaaaccctg gatcttcgaa cattgttata
     1381 cgtccaggct gcaccaataa agacagccat ggttttgatt gccttgtccg cgacatcgag
     1441 ccagaaggtc ccgtaaggat ggacggcatc cgaagcgaag agcatgcgtt gcctccttgt
     1501 cgatcaggca atcagtatcg atcgcatggg taaatcggac cctgagaatt atcgaatcat
     1561 agtaacaaga cttactgcga taaggtgggt cctcagcgag tcaccgattc atccatggtt
     1621 acgcggggat gacgctaagc ttgggcttct tcgcgcagcg catccggggc gtgggcgatc
     1681 cgcatgagcc tggcgtggtc gagccagtcg agcgagcagc gttcgcagtc ggagaggatc
     1741 acgttaccgg gacctgcgta gaagtgcgtc tccatggcct ggcggcagtg cggacaggtg
     1801 aggtggcgct caagttcggc gcggtcgggg ggattgggga taactccgcc gccgtcgttt
     1861 tttctgcgaa gggtttggac caggtcggcg aagaggtcca tgtcgatgag catgccacgg
     1921 cagcggatgc agtacctgag gcgcgcgccc gcgaaggcgg cctcaacgag gccggtgtga
     1981 caggtggggc acagttcatc gccaggttcg ctgaggacgc ggacgccggc gtcgtctttc
     2041 tcgggaaaga cgatgttgcc acagtaggcg caggtcaggc tttcggcgcc ggatgccagt
     2101 tgcatgggag cgccgcagtt agggcaggtc atggaattgg gctctcgttg cagggagaat
     2161 cgtaagtccc ctcgcttcgc tgcgctctgg ccgggataac aagtgcaggg agttcagaac
     2221 cacaccgccg gccctcacag ggttgaaacg gtcactgctg gaggccgcat tccgggcaga
     2281 actttgccgt gcggaccatg ggctttccgc aggagacgca gaacttctgc agcgcggcaa
     2341 tggcagaggc gctggcggaa ttggtgattt cggtggcggt gatggatccc gccagagcgc
     2401 ggccgagcgt gatgccggca ccgatgccga cgccagctcc ggctgttccg ttgggattgg
     2461 cagcagcggc cccgagggcc tgggcggcct ggaattgcgt gtagggtgtg aggttgccgg
     2521 ccatctgcac gccgatgcgc tgatccatga ctttggtgag ctcgtcgggc agggaaagat
     2581 tctcaacgac gaaggatgtg agttcgaggc caagttcggt gaaagagggc tgcacggcgt
     2641 cggcgatttt ctgggagagc gcagcctgat tcttcgccat atcgaggaag gacacgtcgc
     2701 tcgaagcgaa gacttcggtc atgcgggcca caatcgtctg gcggagctgg ccttcaaggt
     2761 cggagacgaa gtaccggtcg cgtgtgccgg agacctcggt gaagaacatg cgcggatctt
     2821 tgatccgata cgcgtaaatc ccatagccgc ggaggcggac ggcgccgaat tccttttcgc
     2881 gcagcgtgat cggcgtgggc gtgccccaca tctggtcgat ctggagacgg gtagagaaga
     2941 agtagacgtc ggatttgaag ggggactgga agcctttgtt ccagtgcatg aggtcggtga
     3001 ggagcgggag gttgcgcgtg ctcagggtgt ggaggccggg gccaaagacg tcggcgatgc
     3061 ggccttcgtt cacaaaggcg gccagctggg attcgcgcac ggtgagttgt gcgccgttct
     3121 ggatctctgc attggccatg ggatagcgcc agacaagagg tccatcttcc ggctcattcc
     3181 actggataac gtcgatgagc tggctggtga gaaattggcg cagggacaac acgggcttcc
     3241 tcctggtgct tccgctggga atgtagcatt cgggggctgg gccgccactc ccacgaagga
     3301 cgcagggggc ggctcaaatc cggacattgg gtcgggattg cgggacggtt atgatgttgc
     3361 aactgaaatg ttgagtagca gggccctgtc gtaatgcacc gcttgttcct gtctgatccg
     3421 gcgttcacga tctcggctgc agcgaggcgg ccatgctgac gtacgcgatc ggctttgtgc
     3481 aggatgtgca gctggtctgt tttgccatcg tgctgacgtc gatggcgatc caggaccggg
     3541 agaacgaaag cctgagctgg ctggccggtg gatacgtggt gggcgcgtgc ggctcgctgc
     3601 tcgacctggg cggacggtgg cttccgcact ggatgtcgat gggcctgttc atggaagcgg
     3661 cgccgatcgg ctacgcgtgc atttacacca gcgtggcggt tttccttcga cgcggcatta
     3721 aagggcgatg gctgtggctg ctgatcgcgc tgggggcgct cccgttcttt ctgcgctgga
     3781 gcgcaacgca gaatttgagc gcgagcgcca cgctggtgga tgggattctg gcggtgcaga
     3841 cggcggctac ttcgttgctg ctgctgtcga caacggatcg ggaaacgcgc tggccgcggc
     3901 gtaccctttc ggcgtttctg gcggtgtatt cggcgattga ggtttcccgg gttgtcattt
     3961 atttggtcac ggggaagatg ccggcggatg tggccccggc agcagagatc gccagcggga
     4021 tcgtttatgt ggtctcgtgc tcagtgctgc cgctggggtt catctggatg atgaacgcgc
     4081 ggctgctgct gcacctgaat cggcagtcga tggtggatcc gctgaccgag ctgctgaacc
     4141 gccggggact gcaggcggcc accgatgtgg agctgtctcg gtacgagcgg acgaggcggg
     4201 atctggctgt agtggtgctg gatctggatt tcttcaagct gcttaacgat cgatacgggc
     4261 atcccggcgg ggacgcgatt ctatgcgcgt tttcggtgtt tctgcgatcg atgattcggg
     4321 agaccgacat tgcgggccgc gtggggggcg aggagtttgt cgtgattctc ccgggcaccg
     4381 acgccgaaac ggcctattcg acgatcgaac ggctgcggca tgcggtggcg gagcacacct
     4441 tcgagctggg cacgcggctg atccacatca cggcgagttt tgggatcacc gtgtcagccg
     4501 ggcgcagcgg cctgacgtgg gagaaactgc agcacgaagc cgatctggcg ctctatgcag
     4561 cgaaggcggc gggccgaaac cggtcgtgtc tgtacgatga atcgctggcg accgctggac
     4621 cgacgctggt ggagaactag agacagatcc gttggcggca ggccgccgca ggggcaagga
     4681 gcgctatact gcactcccca tcgccaggtc tgtctccgag gttttctgtg atgcgattcg
     4741 cgctgatagc cgtgttgtgc gcaatgcctc tcttcgggca atcgacgtct gtgccgtcca
     4801 aaccgaaggt cagagcgatt acaggcttcg ttcgcctcga tcgaagcacg tggcaacagc
     4861 aaattgcgga cacgctttcc gttttgcgca aagcgaagag cgaatttgag gccaggggct
     4921 atgcggtgga gagcgtgcgc atcaccaccc agccactggc cgagctggtg agcggcgaga
     4981 ctgaggaaca ggcgctggcc attctcggcg aactcgaccg cctctcggtg aaggaagaat
     5041 tcctgcccga tgtcgggccc gcgatgctgc acgacggcga cgatccggct ccgatgcacc
     5101 tgcttgagcg ggcgctgtcg acgttgccaa acatcgaagc cagcaccatc atcgcggaca
     5161 gcgacggcat tcactggaag acgatccgcc agacggcgga gctcgtgaaa tacgttgccg
     5221 aacacagccc gcatgctcag ggaaccttca acttcacggc aacggcgatg ctgaagccac
     5281 tgggcccctt ctttcctggg tcgtggcaca acggtgcggg ccagcagttc gcgatcggat
     5341 ttgaaggcgc caacgttgtc gcggatgtat tcacgaaaga taagggaaac tacgacgcag
     5401 ccgttgccga tctgacgcgt gcgctcagcg agcacgctca ggtcgcggac tccatcggcc
     5461 gcaaggtcgc ggcagaaacc ggatggaact atgtcggcgt ggacccgacg ccggcgccac
     5521 tggcggacgt ttcgattggc acggccattg aggactacac cggcgcgcgg tttggatcga
     5581 gcggaacgat gaccgcggcg cgcatcatta ccagcgctgt gaaggcggtg ccggtccagc
     5641 agaccggcta ctccggcttg atggtgccca ttatggaaga caaggtcctt gcccagcgct
     5701 gggccgagtc ggcgtacagc atcgattcgc tgctggcgta ttcagcggtg tgcggcacgg
     5761 ggctcgatac gattccgctg ccgggagatg tgagcatcca gcagatggag cgcatctacg
     5821 gcgatgtggc ttctctggcg acgaagtgga acaagccgct ttcggcacgg ctgcagccgg
     5881 tgcctgggaa gaaggcggga gaccggactg aatttcagga tccgtatttg ttcaacacga
     5941 tgatccaccc gctgccgtga agcaggttca taaagatgag gcgcgagaga aagtctctcg
     6001 cgcctccatt tggaatcctg caatagctca tgccatgtgc tgcaggactt cctcgcgtac
     6061 ttgctcgtcg cggtgttctt ccgccgtgga gcggggcagg gctacggcca gcacgtcttc
     6121 gatacggctg gcgtagcgga tctcgactcc ctcgatctgt tccggcatca ggtcttcttc
     6181 cacgttcaga cggttttctg cgggaagaat gacggttttg acgccggcac gtttcgcggc
     6241 gaggaatttc tccttgatgc cgccgacagg gagcacgttt ccgctcaggg tgatttcgcc
     6301 ggtcatcgcg gtcagcggat ggaccggagt atcggtgaga agcgagacca gcgccgtggc
     6361 catggtcacg cccgcggaag gtccgtcctt ggggatggcg ccggccggca cgtggatgtg
     6421 cagatcggtg tccttgagga gatcttcttc gagaccgagc gatttggcgt tggaccgcac
     6481 ccaggtgagc gcggcctgca tggattcctg catgacctcg ccgatctgtc cggtcatcgt
     6541 gaagccgccc ttgcccttca tgcggttggc ttcgatgaaa agaacgtcgc cgccggccgg
     6601 agtccacgcc agacccacag caaccccggc gcgcttggtc cgctcggcga tttctgtgtc
     6661 gacccgtacc ttgatgccgc cgagaaattc ctggatcacc tcttttgtga tgaccagctt
     6721 ctcggtgttg ccttcagcga tgcgccgcgc ctgcttgcgg cagatggttc caatctgctg
     6781 ctccagcttg cgaacgcccg cttcgcgcgt gtaatggcgg acgatgtagc cgatagagtc
     6841 ctcggggaac tcgatgttct ccggggcgat gccgttctcc ttaatctgcc gggggacgag
     6901 gtagcggaag gcgatatgct tcttctcttc ctcggtgtaa ccctgcagtt cgatgatctc
     6961 catgcggtcg cggaggggct ccggcaccgt gtccagcatg ttggcggtgc agatgaacag
     7021 gaccttggag aggtcgaagg cctgatcgag gtaattatcg cggaaggtgt tgttctgttc
     7081 cggatcgaga atctcgagca gggcggacga tgggtcgcca cggaaatcgc ggcccagctt
     7141 atcgacttcg tccagcatga agac
//
LOCUS       DASZYN010000394         3682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1110959, whole genome shotgun sequence.
ACCESSION   DASZYN010000394 DASZYN010000000
VERSION     DASZYN010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110959"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1540)
                     /locus_tag="VHE33_10130"
     CDS             complement(80..1540)
                     /locus_tag="VHE33_10130"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVT97856.1"
                     /translation="MMRRIAMLAVVLCGAMAWAQNPGEKEPDPLAPFVKVKADSIVLE
                     HVRVIDGTGAPAKDDQTVVIENGKIANVFGGAGNHQFPANARHIDLTGKTIFPGLVGM
                     HEHLFYPLPDAAPDLLPLYGEMADSAPRLYLAGGVTTARTAGSLEPYTDISIKQAIDQ
                     GEIPGPKLFLTGPYLEGAPAIGPQLHALTGPDDAARTVDYWSAEGMTSFKAYMHITPA
                     ELKAAVDHAHEHGFKVTGHLCAVGFKEAASLGIDNLEHGLAVDTEFYSQKKEGVCPGQ
                     HPPQAELAKMDVEGPEIQGTIKDLIAHHVAVTSTLAIFESFVPNRPPLSDEMRVRSSL
                     LPEAWGSYATTRAAIAEHAQDAMWTTLLKMEMQFERDFVKQGGLLMAGCDPTGYGGVL
                     PGFGDQRNLELLVEAGFTPEEAIHLATENGAQWLGEDSRLGTIAVGKAADLVVVDGNP
                     AAHIADVEKVDTVYKDGVGFDPQKLIESVRGLVGLR"
     gene            complement(1637..2362)
                     /locus_tag="VHE33_10135"
     CDS             complement(1637..2362)
                     /locus_tag="VHE33_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97857.1"
                     /translation="MHTHPRVVFLAACCVALGIVFEAHALTHCVFQQAGGRYTGGCGR
                     LFDQTPIMTLVREAAITTGLWRADRHPSSAWGGTMTDDGDTDPLELEVYPDRTGVLRT
                     EYGWFAVKRFEAATAMEFDLEVEKEVEPNSLDAAIIRAAAKFLSSDRVWNRADNRRCA
                     ETATTWSIYCAMEKATQEVTGGFHHRRPALEAVREIVDERTKGRPYHHRLMDYNNDPT
                     THLSDVQSLFTEALARVRREQGS"
     gene            2472..3290
                     /gene="mutM"
                     /locus_tag="VHE33_10140"
     CDS             2472..3290
                     /gene="mutM"
                     /locus_tag="VHE33_10140"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HVT97858.1"
                     /translation="MPELPEVETIANGVNRRVRGDSIVSAWFSHHREPFKTPPAAMAE
                     ALPGLRIGRVRRVGKHIVVDLTAKSARTTSKQLLVHLGMTGRLLVTAPEIPVAPHTHA
                     ILQLASGRELRFVDPRRFGRLQFLEGAGFAGPGHEPLTISLDDFVALFRGRKLAIKAA
                     LLNQSLLHGVGNIYADESLFRAKIRPTRAAGRLTRESLRRLHKALQTVLQAAIRAGGS
                     SVSDYVDADGEAGFFQIQHKVYGRERQPCRVCGRPIRRIVLAGRGTHFCPHCQR"
BASE COUNT          674 a         1246 c         1059 g          703 t
ORIGIN      
        1 ctgaaggcga aatactgggg cgacgatgcg gaatcgcggc tgcgcggcaa gatgggttcc
       61 ggaagccagc cgtaagcgtc taccgcaacc ccaccagccc ccgcaccgat tcgatcagct
      121 tttgcggatc gaatcccacg ccatccttat agacagtgtc gactttctcg acgtctgcga
      181 tgtgcgccgc tggattgcca tcgaccacca cgagatccgc cgctttgccc acggcgatgg
      241 tcccgagccg actgtcttcc cccagccact gcgcgccgtt ttccgtcgca agatggatgg
      301 cctcttccgg cgtgaaacca gcctcgacca gcaactccag gttgcgctga tcgccaaaac
      361 ccggcagcac gccgccgtag ccggtggggt cgcagcccgc catcagcagg ccgccctgct
      421 tcacgaaatc gcgctcgaac tgcatctcca ttttcaacag cgtcgtccac atggcgtcct
      481 gcgcgtgctc ggcgatcgct gcgcgcgttg tcgcgtaact gccccatgct tccggcagca
      541 gcgagctccg gacccgcatt tcgtcactca ggggtggacg attcggcacg aagctctcaa
      601 agatcgccag cgtcgaagtc acagcaacgt gatgagcgat gaggtctttg atcgtgccct
      661 ggatctccgg tccttccacg tccatcttcg ccagctcggc ttgcggcgga tgctgccccg
      721 ggcacacgcc ttctttcttc tgcgaataga actccgtgtc gacggcgagg ccatgctcga
      781 ggttgtcgat gccgagcgac gccgcctctt tgaatcccac cgcgcacaga tgccccgtca
      841 ccttgaatcc gtgctcatgc gcgtgatcga cggcggcctt caactccgca ggcgtgatgt
      901 gcatgtacgc cttgaacgaa gtcatcccct cggccgacca gtaatcgacc gtgcgtgcgg
      961 catcgtccgg gccggtaagc gcgtgcagtt gcggaccaat cgcaggcgcg ccttcgaggt
     1021 acggcccggt gaggaagagc ttcggcccgg gaatctcgcc ttggtcgatc gcctgcttga
     1081 tcgaaatatc cgtgtacggt tccagactcc ccgccgtgcg cgctgttgtg acacctccag
     1141 ccagatagag ccgaggcgcg gagtccgcca tttcgccgta gagaggcaac aagtccgggg
     1201 ctgcgtccgg cagtgggtag aagaggtgct cgtgcatgcc gaccaaaccc gggaagatcg
     1261 tcttgccggt gaggtcgatg tgcctggcat ttgccggaaa ctgatggttc ccggctccgc
     1321 cgaacacgtt ggcaatcttc ccgttttcga tcacgacggt ctgatcgtct ttcgccgggg
     1381 cgccggttcc atcgatgacc ctcacatgct ccagcacgat cgagtcggcc ttcaccttca
     1441 caaacggagc cagcgggtcc ggctcctttt cacctgggtt ttgcgcccac gccatagccc
     1501 cgcacagcac caccgccagc atcgcgatgc gtcgcatcat cattgacctc gctcgaggga
     1561 tctgggaaaa ggcgaatccc cactatccgg gcacaggtcg gcttgcgtca agcgtgcaca
     1621 gcccgatgag acgcttctac gatccctgct cccgcctgac gcgcgccagt gcctccgtaa
     1681 aaaggctctg cacatcgctc aggtgggtcg tcggatcgtt gttatagtcc atcagccgat
     1741 ggtgatacgg acgccccttc gtccgctcgt ccacaatctc gcgaactgct tccagtgccg
     1801 gccggcgatg gtgaaagccg cctgttacct cctgcgtcgc cttctccatc gcgcagtaga
     1861 tactccacgt cgtcgcagtc tccgcgcacc gccgattgtc ggcgcggttc cacacgcgat
     1921 ccgaggacag gaactttgca gctgcgcgga tgatcgcagc gtcgagagaa ttcggttcaa
     1981 cctccttctc tacctccaga tcgaactcca tggccgtcgc cgcctcgaag cgtttcactg
     2041 caaaccatcc atattcggtg cgcagcacgc cggttcgatc cgggtagact tccagctcga
     2101 gcggatcggt gtcgccgtcg tcggtcatgg ttccacccca cgcagaggac ggatggcgat
     2161 cggcgcgcca taaaccagtc gtgatggcgg cctcgcgaac gagtgtcatg atcggcgttt
     2221 ggtcgaagag gcgtccgcag ccgccggtgt agcgcccgcc ggcctgctga aagacacagt
     2281 gcgtgagagc gtgcgcctcg aatacgatcc cgagggcgac acagcaagcg gccagaaaaa
     2341 cgactcgggg atgggtgtgc atcaggggtt cctcgctggt tttcagagat cagcaggttg
     2401 gacgagttcg gccacacgaa gacgcaccac ggatggcgga atgacctcct ggccgtacac
     2461 tcaaatctgc catgcccgaa ctccccgaag tcgaaaccat tgcgaacggt gtgaaccgcc
     2521 gcgttcgcgg cgacagcatc gtttcggcct ggttcagcca tcacagggag ccgttcaaaa
     2581 cgccgcccgc agccatggcc gaagccctcc ctggcctccg catcggccgc gttcgccgcg
     2641 tcggcaaaca catcgtcgtc gacctcacgg caaaatccgc ccgcacgacc tcaaagcagc
     2701 tgctcgtgca tctcggcatg acgggccggc tgctcgtcac cgctccggag attcccgtcg
     2761 cgccccacac ccacgccatc ctgcaactgg cctcgggccg cgagctccgt ttcgtcgatc
     2821 cgcgccgttt cggccggctc cagtttcttg aaggtgccgg tttcgccgga cccggccacg
     2881 agcctctcac catctccctc gacgactttg tggccctctt tcgcggccgc aagctcgcca
     2941 tcaaagccgc gctgctgaac cagtcgctgc tccacggcgt cggcaacatc tacgccgacg
     3001 agagcctgtt ccgcgccaaa atccgcccca cgcgcgccgc cggacggctc acccgcgagt
     3061 cccttcgccg cctccacaag gctctccaga ccgttctcca ggcggccatc cgggccggag
     3121 gctcatcggt ctccgactac gtcgacgcgg acggagaggc cggcttcttc cagatccagc
     3181 acaaggttta tggccgcgag aggcagccct gccgcgtgtg cgggagaccc attcggcgca
     3241 tcgtactggc cgggcggggc actcatttct gccctcattg ccaaagatga attttccagg
     3301 ctgggcggga cccggcaaga ggcgaacgaa agtagtccca cccttgtttt atgaatttca
     3361 ggaccgctcc cctcggcgtt ctacaggatt cggaatgaac tctgccgggc aaacgagggt
     3421 aaatgtatga ttccatggcc aaatacgaaa ggcccctcga ttgcacttag gcatgtcgcc
     3481 agcgacgcat aggcccttgg aagagggccg gtgcgaaaca agattccggc cgaagagagc
     3541 acgtccttcg agattctgcc ccggccggaa tgaacaaatg tcctgatcca tcggttctct
     3601 tagttccagg tcaagggcaa cttcagggag gccagtgact ggctgtcacg cggaaaagcc
     3661 atccatcgga tggcttttct gt
//
LOCUS       DASZYN010000395         6199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_980452, whole genome shotgun sequence.
ACCESSION   DASZYN010000395 DASZYN010000000
VERSION     DASZYN010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980452"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VHE33_10145"
     CDS             <1..796
                     /locus_tag="VHE33_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97859.1"
                     /translation="ALVASFTAYQPRTFAIRLDAPTAHVVPVHTQPVTLQYDLAAAST
                     DGTAVQGGFDGQGNALPAEMLPQTLHFEGVDFHLAPAQNGTPNALIANGQSIHLPQGQ
                     HNRAWILAASIDGDQKADFQVGDRTTTLNIEDWGGFIGQWDDRQWKAGEVEMPARPGR
                     PARTEHDDYAEMTGIRPGYIKRASLAWYCSHHHDASGKNVAYAYSYLFGYSIDLPAGA
                     RTITLPKNDKVRILAISVADENPAVQPSQPLYDTLGRSEPGAVEQP"
     gene            1002..1451
                     /locus_tag="VHE33_10150"
     CDS             1002..1451
                     /locus_tag="VHE33_10150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HVT97860.1"
                     /translation="MARSVNKVILVGNVGKDPEIKVASTGNAIATFSMATTDRTKDSA
                     GNWVDRTEWHSLVAFARTAEIIRDYVKKGSKLYVEGRIQTRSWDDKDGQKKYRTEIIV
                     NDLVLLSGRGDGESAGSSRAGSPYDQRQPAHDDVVQGTEITDDDIPF"
     gene            1513..1677
                     /locus_tag="VHE33_10155"
     CDS             1513..1677
                     /locus_tag="VHE33_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97861.1"
                     /translation="MIAITGVILIVLLVGIFFLFRAMGTMGQRPSAQDRMRHNADDQH
                     RPGPRATGLN"
     gene            complement(1661..2542)
                     /locus_tag="VHE33_10160"
     CDS             complement(1661..2542)
                     /locus_tag="VHE33_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97862.1"
                     /translation="MAAGLRCIVVLLIAATTAPLYSQARAETAHDLVRDVIYNELHDR
                     EHDSHWQYRSECVSSTQNVVREQVETDEGPIFRLLARNGNPLDAAQQDREDARLAEYL
                     QSPAQIAKVDRDHQEDESRLAGMMELLPQAFLFEYQGGPDGHLQRIAFRPNPAFVPAS
                     YEARIVHALAGTFTVDVRTKRMVEMRGTVAERVEFGYGLLGHVEKGGTFDIHRRQVSA
                     EHWKTDLVDVHVQGKILMLKTVGKDEREARSDFRPVPVGTTLEQAREMLNETVDRQTR
                     ARLVQPGNAAKGISSDR"
     gene            2705..3469
                     /locus_tag="VHE33_10165"
     CDS             2705..3469
                     /locus_tag="VHE33_10165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVT97863.1"
                     /translation="MPLTLSVAIITKNEEANLARTLASVRWADEIVIVDNGSTDRTPD
                     IAREFGAKLFTEEWKGFGAQKNSAIAKCASDWVLALDADEEVSTDLAAEIRNLLEGQP
                     VHEAYFIPRRNFFLGRWMRHGGYYPDPKLRLFRRETAQFEERAVHETVHATVPAGRLR
                     GDLLHYAYPTLEGYLEHMNRYSTLGAAQVIARGKTSPSIFAFLWNVLIVPLATFKYNY
                     FLRLGFLDGREGLLQHLYHSAYVSWKYAKAWEKARY"
     gene            3549..3713
                     /locus_tag="VHE33_10170"
     CDS             3549..3713
                     /locus_tag="VHE33_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97864.1"
                     /translation="MAKRQRVFKGGAEYEVTGATKRKRRLVFINTVKIEGREYLLFRP
                     VKKISKLING"
     gene            3765..5549
                     /locus_tag="VHE33_10175"
     CDS             3765..5549
                     /locus_tag="VHE33_10175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HVT97865.1"
                     /translation="MPDKATHPRDSLLTDLTSDRRPSPEELRWAEQTLEPALAKSPEH
                     AIGKPTGVNRDTSGAARFTTISGAPIRRLYTEADLPEDWRENQDAYLGAPGEPPYTRG
                     LQPTGYRGRLWTMRQFSGFASAEETNRRYKYLLEGGAGGLSVAFDLPTLMGCDSDDPK
                     SEGEVGKCGVAIDSLEDMETLFAGIPLATTTVSMTINSPAAVLWAMYLVVAERQGADW
                     QQVSGTLQNDILKEYIAQKEYLYPPAPSMRLVVDTLEFGARHTPRFNPISISGYHIRE
                     AGSTALQELAFTLYDGIEYVEWALRRGLAVDDFAPRLSFFFNAHNDFFEEIAKYRAAR
                     KIWYRVMTERFGAKNPRSTWMRFHTQTAGVSLTAQQPKNNIARVALQAMAAVLGGTQS
                     LHTDGFDEALALPTEEAARIALRTQQIIAHESGVADIIDPLGGSYFLEKLTLDMERDA
                     FRIFEKLDAMGGMVKAIEAGYPQKEIAEASYAYQRAVEAGEKIIVGVNQYATGDQPTR
                     TLYIDESVRDAQAARLKALRARRSGEAVQRSLHALRRVAAQEPEPAANGLSAANTMPF
                     ILDCVRSYATLGEICNALHQAYGGWEEA"
     gene            5633..6139
                     /gene="coaD"
                     /locus_tag="VHE33_10180"
     CDS             5633..6139
                     /gene="coaD"
                     /locus_tag="VHE33_10180"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HVT97866.1"
                     /translation="MNTVKAIYPGSFDPVTNGHLDLIARAASIFDHLVVAILRNSTKN
                     PLFTVEERVAMLSEGTAEFGNVSVSTFDGLLVDFAREQRAHAVVRGIRAISDYEYEFQ
                     MALMNRRLSPDVETIFLMPDAKYSFVSSRLVKEVFRLGGSVEGLVPKFVIERMKDKSR
                     TRIAESLR"
BASE COUNT         1206 a         2144 c         1706 g         1143 t
ORIGIN      
        1 cgccctggtg gcgtccttca cggcgtatca gccgcggacc ttcgcgattc ggctcgatgc
       61 cccgacggcc catgtggtgc cggtgcacac ccagccggtg accctgcaat acgatctggc
      121 cgcggccagc acggacggca ctgctgtcca gggcggcttc gatggccagg gtaacgcgct
      181 tccagcggag atgctgccgc agaccctgca tttcgagggc gtggatttcc atctggcgcc
      241 cgcgcagaat ggtactccca atgctctgat tgcgaatggc cagagcattc atctgcctca
      301 ggggcagcac aatcgcgcct ggatcctggc ggcgtccatc gacggcgatc agaaagccga
      361 tttccaggtt ggcgaccgca ccaccacact gaacatcgag gactggggcg gattcatcgg
      421 gcaatgggac gatcggcaat ggaaagccgg agaggtcgaa atgcccgccc gccccggccg
      481 tcccgccagg accgagcatg acgactacgc agagatgacg ggcattcgcc ctggctacat
      541 caaacgcgcc tccctggcct ggtactgttc gcaccaccac gacgcgtcag gcaagaacgt
      601 tgcgtatgcc tactcctacc tcttcggcta ttcgatcgac ctgccggccg gagcgcggac
      661 catcactctg ccaaagaatg acaaggtgcg catcctggcg atctcggttg ccgacgagaa
      721 tcccgcggtt cagccttcgc agccgctcta cgacacgctg ggccgctccg aacccggggc
      781 agtggagcag ccgtaaccat cgtttccgca gcgggtccgc cgccgggccc gccgcaccct
      841 tctcaatcaa cagttggaag agcttgcata gggcgggttt cgggggggca caccaaggtc
      901 ccgtgtccag ggtaacgtgt gcgcagcggc ccgcgctgct tcgtgccctc ccttcctgct
      961 atccttcatc agacaaaacg atccaggaga gagaatccgc catggccagg agcgtgaaca
     1021 aagtcatcct tgtgggaaac gtcggcaagg atccggaaat caaagtcgcc tccacgggca
     1081 acgcgatcgc caccttttcc atggcgacca cggatcgcac caaagactcg gccggcaact
     1141 gggtcgatcg cacggagtgg cacagcctgg tcgcctttgc ccgcaccgcc gaaatcatcc
     1201 gcgattacgt gaagaagggc tccaaactct acgttgaagg ccgcatccag acccgctcgt
     1261 gggacgacaa ggacggccag aagaagtatc ggaccgagat catcgtcaac gatctggtgc
     1321 tgctctcggg ccgcggtgat ggcgaatcgg cgggctcctc gcgtgccgga tccccttatg
     1381 accagcggca gcccgcacac gacgatgtcg tccagggcac cgagatcacg gacgacgaca
     1441 ttcccttcta gacaacgttt ccggataacc cttttccagc ccggcctctc ctatggaaca
     1501 ggagagttcg ctatgattgc catcaccggc gtcatcctca tcgtgctgct ggtggggatt
     1561 ttcttcctct tccgtgcgat ggggacaatg ggtcagcggc cctcggcgca ggaccggatg
     1621 cgtcacaatg cagatgacca gcaccgccca ggcccccgtg ctaccggtct gaactaattc
     1681 ctttcgccgc attcccgggc tggaccagcc gcgcccgcgt ctgccgatcg accgtctcgt
     1741 tcagcatttc cctggcttgt tccagggtgg ttccgaccgg caccggccgg aaatccgacc
     1801 gcgcctcgcg ctcatccttg cccaccgtct tcaacatcag aatcttcccc tggacgtgaa
     1861 catcgacaag gtccgtcttc cagtgctccg cgctcacctg gcgccggtga atgtcaaacg
     1921 tcccgccttt ctcgacgtgc cccagcaagc cgtatccaaa ttcaacccgt tccgcaaccg
     1981 tcccgcgcat ttccaccatc cgtttcgtcc gcacgtcgac ggtgaatgta ccggccaggg
     2041 cgtgaacaat ccgtgcttca taacttgccg gcacaaaggc cggattgggg cgaaaggcga
     2101 tccgctgcag gtggccgtcg gggcctcctt gatattcgaa cagaaatgcc tggggcagca
     2161 gttccatcat gcccgccagc cggctctcat cttcctgatg atcgcggtcc accttcgcaa
     2221 tttgcgcggg actctgcagg tattctgcca accgcgcgtc ctcccgatcc tgctgtgctg
     2281 cgtccagcgg attaccgttc ctggccagaa gacggaaaat cggcccttcg tccgtctcca
     2341 cctgctcgcg caccacgttc tgcgtcgaag aaacgcactc gctccgatat tgccagtggc
     2401 tgtcgtgctc ccggtcgtgc agttcgttgt agatcacgtc ccgtaccaga tcgtgggccg
     2461 tctcggcacg cgcctggctg tacagtggag cggtcgttgc cgcgatcagg agaacaacaa
     2521 tgcagcgcag acctgcagcc atggggaatc tccagtctcg taggatgacc gttcgccccc
     2581 agcttgctgc cacagctttg cgaatctggt gaatcgtctc tgaagattcc gtgacacgct
     2641 gcgggtggaa tctgacttgt tacacttccg ccagagcgga gaagcgtctc ttctcgatcg
     2701 actcatgcca ctcactctct cggtcgccat cattacaaaa aacgaggaag cgaacctggc
     2761 gcgcaccctg gccagcgtgc gctgggccga tgagatcgtc attgtcgaca acggctcgac
     2821 cgaccgcacc cccgacatcg ctcgtgagtt cggcgcgaaa cttttcaccg aagagtggaa
     2881 aggcttcggc gcacagaaga actccgcgat cgcaaagtgt gcctcggact gggtgctcgc
     2941 cctcgatgcc gacgaggaag tcagtacgga tctggctgcc gaaattcgca atctgctcga
     3001 aggccagccc gtgcatgagg cgtatttcat cccccgccgc aacttctttc tcggtcgctg
     3061 gatgcggcat ggcggctact acccagaccc caagctgcgc ctctttcgcc gcgagacggc
     3121 acagttcgag gagcgggccg tccacgagac ggtccacgct acggtcccgg ccggacgcct
     3181 tcgcggcgac ctcctccact acgcctatcc gacgctggaa ggctaccttg agcacatgaa
     3241 tcgctacagc acgctcggcg ccgcccaggt catcgcccgc ggaaaaacca gcccatccat
     3301 ctttgccttt ctctggaatg tcctgatcgt tcccctcgcc accttcaagt acaactactt
     3361 cctgcgcctc gggtttttgg acggccgcga aggcttgctc cagcacctct accactccgc
     3421 ctacgtgagc tggaagtatg ccaaggcctg ggaaaaggcg cggtattgaa atcagcagga
     3481 ggaaaagcct ccacccacgc gaaactgcta gattaacggc agctatcagg ggtcctgttg
     3541 gaggaactgt ggcaaaacgt cagagggtgt ttaagggcgg cgctgaatat gaggtaaccg
     3601 gcgcaacaaa acgcaagcgt cgtctggtct ttatcaatac cgtgaaaatc gagggtcgtg
     3661 agtatctcct tttccggccg gtaaagaaaa tctccaagct gatcaacggc tagtcgtcgt
     3721 cctggcatcg cctctgatag ggattaaggg ttccaccctc cgtcatgccc gacaaggcaa
     3781 ctcatcctcg cgattcgctc ctcaccgacc tcacctcgga tcgccgtccg tcgccggagg
     3841 aactccgctg ggccgagcag accctggaac ctgcgctcgc gaagtccccc gagcacgcca
     3901 tcggcaagcc gaccggcgtc aatcgcgata catccggagc cgcacgcttc accaccattt
     3961 caggtgctcc cattcgccgc ctctacactg aagccgatct gccggaagat tggcgcgaaa
     4021 accaggacgc ttatctcggc gcgcctggtg agccgccgta cacgcgcggc ctgcagccca
     4081 ccggctatcg tggccgcctc tggaccatgc gccagttctc cggcttcgcc tcggctgaag
     4141 agaccaatcg ccgttacaaa tacctgctcg aaggcggagc cggcggcctc tccgtcgcct
     4201 tcgacctgcc cacgctcatg ggctgtgact ccgacgaccc gaagagcgaa ggcgaagtcg
     4261 gcaagtgcgg cgtcgccatc gactcgctcg aagacatgga gacactcttc gccggcatcc
     4321 cgctcgccac aacgaccgtc tccatgacca tcaactcgcc cgccgcggtt ctgtgggcga
     4381 tgtatctggt cgtcgccgag cgccagggcg ccgactggca gcaggtttcc ggcaccctgc
     4441 agaacgacat cctcaaggag tacatcgcgc agaaggaata tctctatccg ccggcgccct
     4501 ccatgcgtct cgtcgtcgat acgctggagt tcggcgcgcg ccacaccccc cgcttcaatc
     4561 ccatctccat ctccggatac cacattcgcg aagccggttc gaccgcgctc caggaactcg
     4621 ccttcactct gtacgacggc atcgagtatg tcgaatgggc tctccgccgc ggcctcgccg
     4681 tcgatgactt cgccccccgc ctcagtttct tcttcaacgc ccacaacgac ttcttcgagg
     4741 agatcgccaa gtaccgcgcc gctcgcaaaa tctggtatcg cgtgatgacc gagcgcttcg
     4801 gcgccaaaaa tccgcggtcc acgtggatgc gcttccatac acagaccgcc ggcgtctcgc
     4861 tgacggcaca gcaaccaaag aacaacatcg cccgcgtcgc gctccaggcc atggctgccg
     4921 ttcttggcgg gacgcagtcc ctccacaccg acggcttcga cgaggccctg gcgctgccca
     4981 ccgaagaagc cgcgcgcatc gcgctccgca cccagcaaat catcgcccat gagtcgggcg
     5041 tcgccgacat catcgatcct ctcggcggct cgtacttcct cgaaaaactc accctcgaca
     5101 tggagcgcga cgccttccgc atcttcgaga aactcgacgc catgggcggc atggtgaagg
     5161 ccatcgaagc cggttatccg caaaaggaga tcgccgaagc cagctacgcc taccagcgcg
     5221 ccgtcgaagc cggcgagaaa atcatcgtcg gcgtcaatca gtacgccact ggcgaccagc
     5281 ccacccgcac cctctacatc gacgagagcg tacgcgatgc gcaggcagcc aggctcaagg
     5341 cgctgcgtgc caggcgctcc ggcgaagccg tccagcgctc cctccacgct ctccgccgcg
     5401 tcgcagcgca ggaacccgag cccgctgcga acggcctctc cgctgccaat accatgccct
     5461 tcattctcga ctgcgtgcgc agttacgcca ccctgggcga aatttgcaac gccctgcacc
     5521 aggcatatgg cggatgggaa gaggcctgac accgcctcct gcttcattcc tgtcgcaaag
     5581 cgatacgctc cttcgctgca ccccgcctca aaccggatag aatgcaactg gcgtgaacac
     5641 cgtcaaagcg atctaccccg gcagcttcga tccggtgacc aacggccacc tcgacctcat
     5701 cgcgcgcgcc gccagcatct tcgatcacct ggtcgtagcg atcctgcgca actccaccaa
     5761 aaatccgctc ttcaccgtcg aggaacgcgt cgccatgctc tccgagggca cagcggaatt
     5821 cggcaacgtc tccgtctcca ccttcgacgg cctgctcgtc gactttgccc gcgagcagcg
     5881 cgcacacgcc gtcgttcgcg gaattcgcgc catcagcgac tatgagtacg aatttcagat
     5941 ggcgctcatg aatcgccgtc tctcacccga cgtcgaaacc atcttcctga tgccggatgc
     6001 gaaatactcc ttcgtcagct cgcgcctcgt caaggaagtg ttccggctcg gtggctcggt
     6061 cgaagggctc gtcccgaagt tcgtcatcga gcgcatgaaa gacaagagtc gcacccggat
     6121 cgccgagtcg cttcgctaag cgctaacgag ccctgctact cttcatctct tcaaaggcac
     6181 gaagcgccgc agcgcgtgc
//
LOCUS       DASZYN010000396         2823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_209502, whole genome shotgun sequence.
ACCESSION   DASZYN010000396 DASZYN010000000
VERSION     DASZYN010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209502"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VHE33_10185"
     CDS             <1..499
                     /locus_tag="VHE33_10185"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT97867.1"
                     /translation="QKQRTLFALALCGNPDLIFLDEPTVGMDIESRRALWDEVRALSA
                     AGKTVLLTTHYLEEADMLAHRIVVINKGHVVCEGTPAEIKHRVSGRRIRCITQLDPEF
                     LATLPSVADVQQDREAVVILAEHPEQVVREMLLRDETLHGLEVSAAALEDAFLALTSS
                     EQPQT"
     gene            718..1500
                     /locus_tag="VHE33_10190"
     CDS             718..1500
                     /locus_tag="VHE33_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT97868.1"
                     /translation="MATNTIIAAPPLTFGYYARIFRKETQYEFVRMLRTRTFSLSVIG
                     FPVMFYLLFGTSNRHNMFARYLMPSYSCMGVVSACLFGIGMGIALERAQGWLELKQAS
                     PMPRFAYLAAKTLSCMAFALVIVATLVTLGVTVGGVTLTVTEALRLAAVVLAGSLPFT
                     AMGLLIALLVPPNAGPGVINLIYLPMSFASGFWMPVNYLPHWLQIITPALPTYHLAQI
                     ALNIFGYAQSGSMLVHWEALAGFTLLMLGAAWIIFMHSEAKA"
     gene            1497..2723
                     /locus_tag="VHE33_10195"
     CDS             1497..2723
                     /locus_tag="VHE33_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HVT97869.1"
                     /translation="MTSSDPKPEIEAPLCRAAALPDFLGSPVAEIPETVTGKVQSAEA
                     DFRGSVFMKEHGHGRFYDFIWLVYSVFFIFDPIQQHSTKVWVEFAIAYGIFLAIYCGL
                     VFSRSHRTQYWLLVGMGLLAMAYYPFNGGSCGMFIYVAAFVPFIAESILVSVGTLIAA
                     TLTMAGEGYYLHISPWSWGICSFFAVAVGAGNLLAAQRMRANKKLGLAHEQIAHLAKL
                     AERERIARDLHDVLGHTLSVVVLKSELAGKLMQRDPERARHEIGEVEQIARKALSEVR
                     EAIRGYRSEGLAAEIARAKKTLDAAGVTLECGVKPPQLAPAEETVLSLIVREAVTNIV
                     RHAQASHCRLEFASNGFGTALTVEDDGRGGVREEGNGLRGMRERVESLGGKLRIDSGQ
                     GTRLLVEIPPQTVEPS"
BASE COUNT          511 a         1013 c          749 g          550 t
ORIGIN      
        1 acaaaagcag cgcactctct tcgctctcgc tctttgcggc aatcccgacc tcatcttcct
       61 cgacgagccc accgtcggca tggacatcga gtcccgccgc gccctctggg acgaagtccg
      121 cgccctttcc gccgccggca aaaccgtcct gctcaccacg cattacctcg aagaagccga
      181 catgctggcc caccgcattg tggtcatcaa caaagggcac gttgtgtgcg aaggcacacc
      241 tgctgaaatc aagcatcgcg tctccggccg tcgcattcgc tgcatcacgc agctggaccc
      301 cgaattcctc gcaaccctgc cctccgtcgc agacgttcag caggatcgcg aagccgtcgt
      361 catcctcgcc gaacaccccg agcaggtcgt ccgcgaaatg ctcctgcgcg atgagaccct
      421 ccacggcctc gaagtctccg ctgccgctct cgaagacgcc ttcctcgccc tcaccagttc
      481 ggagcagccg caaacctgaa gatcgccttg tatccggcac gaatcaacgc gcaccccata
      541 tctgcccgct gttggcagat gtgggagaga agatctcaat cagcccatcc ccattcgtgg
      601 ggcttcagcc ccacgaagac ggtagaagcc ccgggcttta gcccggggaa caagtcgcag
      661 caagaagaag gggcttgagc cccggagttt tgaaagcacg attcgaggag aaacatcatg
      721 gccaccaaca caatcatcgc cgcgcccccc ctcactttcg gctattacgc gcgcatcttc
      781 cgcaaggaaa cccaatacga attcgtccgc atgctgcgca cgcgcacctt ctcgctctcc
      841 gtcatcggct ttccggtgat gttctacctc ctcttcggca cctcgaaccg ccacaacatg
      901 ttcgcgcgct acctcatgcc cagctacagc tgcatgggcg tcgtctcagc gtgcctgttc
      961 ggcatcggca tgggcatcgc gctcgagcgc gcccagggct ggctcgaact caagcaggcc
     1021 agtcccatgc cgcgctttgc ttatctcgcc gccaaaacgc tcagctgcat ggccttcgcc
     1081 ctggtcatcg ttgccaccct ggtcaccctg ggcgtgacgg tcggcggcgt cactctcacc
     1141 gtcacagaag ctcttcgctt ggccgcggtc gtcctggccg gatccctgcc gttcaccgcc
     1201 atgggactgc tcatcgcgct gctggttccc cccaacgccg ggccgggagt cattaacctc
     1261 atctaccttc ccatgtcctt cgcctccggc ttctggatgc ccgtgaacta cctgccccac
     1321 tggctgcaga tcatcactcc ggctcttccg acctaccacc tcgcccagat cgccctgaac
     1381 atctttggtt acgcccagtc cggcagcatg ctcgtgcact gggaagccct cgcaggattt
     1441 accctgttga tgttgggcgc cgcatggatc atcttcatgc acagcgaggc gaaggcttga
     1501 cgtcatcgga tcccaaaccg gaaatcgaag ccccgctctg tcgggcagcc gcgctgcccg
     1561 atttcctcgg cagccccgtg gctgagattc ccgagacggt tacgggaaag gtgcagtccg
     1621 ccgaagcaga tttccgtggc agcgtgttca tgaaagaaca cggccatggc cgcttctacg
     1681 acttcatctg gctggtgtac tccgttttct tcatcttcga ccccatccag cagcacagca
     1741 ccaaagtatg ggttgagttc gccattgcct acggcatctt cctggccatc tactgcggtc
     1801 tggtcttctc ccgttcgcac cgtacccaat actggctcct ggttgggatg ggccttctcg
     1861 ccatggccta ctatcccttc aacggcggct cctgcggcat gttcatctac gtcgcagcgt
     1921 tcgttccgtt catcgcggaa tccattttgg tcagcgtggg cactcttatc gccgccacgc
     1981 ttaccatggc cggcgaaggc tactaccttc acatctcgcc ctggtcgtgg ggcatctgtt
     2041 cgttctttgc tgttgcggta ggcgccggca acctgctcgc cgcccagcgg atgcgcgcca
     2101 acaagaagct cggcctcgcg cacgagcaga tcgcccacct cgccaagctg gccgaacgcg
     2161 aacgcatcgc ccgcgatctc cacgacgtcc tcggccatac gctctccgtc gtcgtcctca
     2221 aatccgaact ggccggcaag ctcatgcagc gcgatccgga gcgcgcccgc cacgagatcg
     2281 gcgaggtcga gcagatcgcc cgcaaagccc tgagcgaagt ccgcgaggcc atccgcggct
     2341 atcgctccga aggcctcgcc gccgaaatcg cgcgcgcgaa gaagaccctc gatgcagccg
     2401 gtgtcaccct cgaatgcggt gtgaagcctc ctcagctcgc acccgccgaa gaaaccgtcc
     2461 tctcgctaat tgtgcgcgag gcggttacca atatcgttcg ccacgcccag gccagccact
     2521 gccgtctcga attcgcctcc aatggcttcg gcacggctct cacggtcgaa gacgacggac
     2581 gcggcggcgt tcgcgaagaa ggcaacgggc tccgtggcat gcgcgagcgc gtcgaatccc
     2641 tgggcggaaa acttcgcatt gactccggcc agggaactcg cctcctcgtc gagattccgc
     2701 cacaaaccgt cgagccatca tgaagaagat ccaggtgatc gtcgccgaag accaggcgat
     2761 ggttctcggc gctcttgcgg cgctgcttga aaccgagccc gacatcgctg tctgcggccg
     2821 cgc
//
LOCUS       DASZYN010000397         5526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_336615, whole genome shotgun sequence.
ACCESSION   DASZYN010000397 DASZYN010000000
VERSION     DASZYN010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336615"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..3379)
                     /locus_tag="VHE33_10200"
     CDS             complement(137..3379)
                     /locus_tag="VHE33_10200"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVT97870.1"
                     /translation="MKRGSHGVAGQLAHSFINSKLTPLTIAAALALGLLAVIAIPREE
                     EPQILVPMLDVMTAMPGASPQEVEQRVTNPVESLMHEIPGVEYVYSISSPGQSLVIVR
                     FLVGTSQEDALVKVYSKLYSSADRMPQGASQPLIKARSIDDVPILALTLWGEHYTGYQ
                     LRQMADEVEHTIRQIPDVSETKVLGGQPRAMRVILDSTKLAGFGFSAGDVVARLQAAN
                     ARTQAGEFAENNQQVRVDAGNLFRDARDLENVVLGVANGQPVYLRDVASRIVDGPAEA
                     QDYVLFGSAQRHGSAAAAKEYPAVTITVAKRKGTNATDIANATLARVQAMKGVSLPAD
                     LTITTTRNYGATAKAKSDTLLEHLLLATLSITILIALFLGWRESGVVLLAIPVTLGLT
                     LAVFYLLGYTLNRVTLFALIFSIGILVDDAIVVVENMVRHFRMPINARRPLSEVAAEA
                     VAEVGNPTILATFTVIAAILPMAFVRGLMGPYMRPIPVGASVAMLFSLVVAFVVSPWA
                     ALRLLGRRHLAGAHTLTPEKEGWLVRFYRRVMHRLMRTARTRWTFLGVVVLLLLLACA
                     LVPMKAVLVKMLPFDNKSEFQVIVNMPDGTPLEQTTHVAQSLGAYLSRQPEVENYQIY
                     SGTSGPFNFNGLVRHYYLRRQPNQADIQVNLLPAGERSAQSHAIARRLRPDLNSIAQA
                     WGARLQVSEVPPGPPVLQTLVAEIYGPSLDGQIDVARQVRQVFRQTQGVVDADWYMQD
                     PQSQLDLRVDEAKAALHGIPVAEVAQTLALATSGAQAGLLHVPESREAVPATVEFDRA
                     ERSSEQGLENIRLRGSNGSMVSLRELVSVEHTTLAPDIYHKNLKRVIYVTGDVAGAEE
                     SPVYAIMKMNRALDQLRLPDGYSLARYNSVQPESTDHFSMKWDGEWQITIEVFRDLGL
                     AFAAVLILIYVLVVGWFKSFTVPLAIMIPIPLTLVGILPAHAMLGAFFTATSMIGFIA
                     GAGIIVRTSIIVVDFIELRRREGMPLAEAVIDAGAIRLRPVLLTAAGVIVGASVILAD
                     PIFQGLGLSLIAGGVASTFLSWPAVPVLYYMLHAHKNVQPAPIEHQEVAS"
     gene            complement(3376..4497)
                     /locus_tag="VHE33_10205"
     CDS             complement(3376..4497)
                     /locus_tag="VHE33_10205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVT97871.1"
                     /translation="MKRSLYLASAIVSAWIALGCSRSAPVPAAPPTAQAQLVHSAAAN
                     TAQTIQMTGTVHAKETAMISAQVPGRLRSVQVQAGGHVRAGQLLATLDDAAMQSALGQ
                     TTAAQTALEKQRAAAQANAQLAAGTLARYEILKAQKSVSPQEFDEVEKRSQGATLQLQ
                     ALEAQSRGAQAAVEGARTQLGYATLHAPFAGVITARLADPGTLAVPGVPLLQLDREGP
                     LQVFTTVDESIIGAVHIGMKVPVSAGGIHDADLNGTVAEIVPAADPVSHSFLVKLDLP
                     PTKNLRAGMYAAASFPGAVKSMILAPQSAVVMRGSLACVYALDADGIAQLRYVTLGNR
                     HGDEVEVLSGLAAGEALVDKPGDRDLAGRLIQPANGAQP"
     gene            complement(4494..>5526)
                     /locus_tag="VHE33_10210"
     CDS             complement(4494..>5526)
                     /locus_tag="VHE33_10210"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HVT97872.1"
                     /translation="PTGNFATRFTGQWMIFNAFETQQQIHAARLGAESAAAVEKATDQ
                     GIVFQVVQAYQAVLHAQRQLDLAQHQQDTAEALVRDAGTRVKAGLAIDSDLLSAQVNL
                     AARQQENIAAQGSVETAWAELETAMGGAVNPRPQLQPIDAKTFSGGAFEEDLARALAA
                     RPDLTALRKQTAAQEQAAKAARSDFFPQLSASGNWETDRPTFAGDGGNNWVAGVQLTL
                     DILPLSKRAHFAEARAAQAKAAAEERSQEQQIRLAVSRAFTGHQTTERMVAASRAAME
                     QAAEGLRILHNRYDAGLATMTDLLHAEDAERQSQNNYWHAAYGNTVAWAALLYATGQL
                     TPDTAETLQ"
BASE COUNT          967 a         1699 c         1897 g          963 t
ORIGIN      
        1 ttggtgaacg ccgactgcag aagattcagg ccaacgaagg ccgtgagcca cagccagtac
       61 ggggaaaacc agtgcgccag ggcgagcgag agcagaacga tgaccccggc cagcagacga
      121 acggcgcgtt cgaccgtcat gatgcaacct cctgatgctc tataggagca ggttggacgt
      181 tcttgtgagc gtgaagcatg taatagagaa cgggaaccgc gggccaggat aagaaagtgg
      241 aagcgacgcc gcccgcgatg agcgagaggc cgaggccctg aaagatggga tcggcgagaa
      301 tgacgctggc gccgacgatg acacctgcgg cggtgagcaa cacaggacgc agacggatag
      361 cgcccgcgtc gatgacggct tccgccagag gcatgccctc gcggcggcgg agttcgatga
      421 agtcgacgac gatgatcgaa gtgcgcacga tgatgcccgc gccggcgatg aagccgatca
      481 tcgatgtggc ggtgaagaac gcgccgagca tggcgtgggc gggcaggatg ccgaccagcg
      541 tgagcggaat tggaatcatg atcgccaacg gcacggtgaa ggacttgaac cacccgacga
      601 cgaggacata gatcaggatg agcaccgcgg cgaaggccag gccgaggtcg cggaagactt
      661 cgatggtgat ctgccactcg ccgtcccact tcatggagaa gtggtcggtt gactcgggct
      721 ggacggagtt atagcgagcg agggagtagc catcgggcag ccggagctgg tcgagcgcgc
      781 ggttcatctt catgatcgcg tagaccgggc tttcttcggc gccggccaca tcgcccgtta
      841 cgtagatcac gcgcttcaga ttcttgtggt agatgtccgg ggcgagggtg gtgtgctcca
      901 cgctgaccag ctcgcggagg gagaccatgc tgccgttgct gccgcgcagg cggatgtttt
      961 cgaggccctg ttcggaggag cgctcggcgc ggtcgaactc gacggtggca ggaacggcct
     1021 cgcgcgattc gggcacgtgc agcaggccgg cctgcgcgcc ggaagtggcc agcgccaggg
     1081 tctgcgccac ctcggcgacg ggaattccgt gcagcgccgc cttcgcttcg tccacgcgca
     1141 gatccagttg cgactgcgga tcctgcatgt accagtcggc gtcgacgacg ccctgggttt
     1201 gccgaaacac ctgcctgacc tggcgggcga catcgatttg gccgtcgagg ctcggtccat
     1261 agatttcggc caccagggtc tgcagcaccg gcggccccgg cgggacctcg gagacctgaa
     1321 ggcgcgcgcc ccaagcctgg gcaatgctgt tcaggtcggg gcgcagccgg cgcgcgatgg
     1381 cgtggctctg cgcgctgcgc tcaccagccg gcaggaggtt cacctggatg tcggcctggt
     1441 tgggctggcg gcgaagatag tagtggcgga cgaggccgtt gaaattgaag gggcctgagg
     1501 tgccggagta aatctgatag ttctcgacct ctggttgccg gctcagataa gcgcccagcg
     1561 actgcgcgac gtgggtggtc tgctccagcg gcgttccgtc gggcatgttg acgatgacct
     1621 ggaactcgct cttgttgtcg aagggcagca tcttgacgag gacggccttc attggcacca
     1681 gagcgcaggc aagcagcaac agcagaacaa cgaccccgag gaaggtccag cgcgtgcgcg
     1741 ccgtccgcat cagccgatgc atgacgcgcc gatagaagcg aaccagccag ccttcttttt
     1801 ccggcgtgag cgtgtgcgcg ccggcgagat gtctgcgccc cagcaggcgc agcgcggccc
     1861 agggtgagac cacgaaggca acgaccagag agaaaagcat cgccaccgaa gcgccgacgg
     1921 gaatcggccg catgtagggg cccatgagcc cgcgcacaaa ggccatgggc aggatggccg
     1981 cgatgaccgt gaacgtggcg aggatggtgg gattgccgac ttcggccacg gcttcggcgg
     2041 cgacttcact gagcggacgg cgcgcgttga tgggcatgcg gaagtggcgc accatgtttt
     2101 cgacgacgac gatggcatcg tcgacgagga tgccgatgct aaagatgagc gcgaacagcg
     2161 tgacgcggtt cagggtgtag cccagcagat agaacacggc cagggtcagt cccagggtga
     2221 ccgggatggc gagcagaacg acgcccgact cacgccagcc gaggaacagc gcgatgagga
     2281 tggtgataga gagggtcgcc agcagcaggt gttccagcag cgtgtcggat ttcgcttttg
     2341 cggtggcgcc gtagttgcgc gtggtggtga tggtgagatc ggcggggagg gagacgccct
     2401 tcatcgcctg tacgcgggcc agcgtggcgt tggcgatgtc ggtggcgttg gtgcccttac
     2461 gcttggcgac ggtaatcgtg accgccggat attctttggc cgccgcggcg ctgccgtgtc
     2521 tttgcgcgga tccgaacagc acatagtcct gcgcctcggc aggaccatcg acgatgcggc
     2581 tggcgacatc acgcagatac accggctggc cgttggccac gccgagcacc acgttctcca
     2641 gatcgcgggc gtcgcgaaag agattgcccg cgtcgacgcg gacttgctgg ttattttcgg
     2701 cgaattctcc cgcctgggtg cgggcattcg ctgcctgcag tcgagcgacg acgtccccgg
     2761 ctgagaagcc gaagccggcc agctttgtgc tgtcgagaat cacacgcatc gcccgcggct
     2821 ggccgccgag caccttcgtc tcggagacat cggggatttg gcggatggtg tgctcgacct
     2881 cgtcggccat ttggcgcagc tgatagccgg tgtaatgttc accccacagg gtcagcgcca
     2941 ggatgggaac gtcgtcgatg gagcgggcct tgatgagcgg ctgcgatgca ccctgcggca
     3001 tgcggtcggc gctggagtag agcttgctgt agaccttcac cagcgcgtct tcctgagaag
     3061 taccgacgag gaagcggacg atcaccaggc tctgccccgg gctggagatg gagtacacat
     3121 attccacgcc ggggatttca tgcatcaggc tctcaacagg attggtgacg cgctgctcga
     3181 cctcctgggg cgaggctccc ggcatcgctg tcatcacatc gagcatcggc accagaatct
     3241 gcggttcttc ttcgcgcggg atggcgatga cggccaacag acccagagcc agcgcagcgg
     3301 cgatcgtcag cggcgtgagc ttcgaattga tgaacgagtg cgccagctgt ccggcgacgc
     3361 catggcttcc ccttttcatg gttgcgcccc gttcgccggc tggatgaggc gtccggccag
     3421 atcgcgatcg ccgggcttgt ccacgagcgc ttcaccggcg gccaggccgg acagaacctc
     3481 cacctcatcg ccgtgccgat tgccgagggt cacgtagcgc agctgggcga tgccgtcggc
     3541 gtcgagtgcg tacacgcagg cgagcgagcc gcgcatcaca accgccgact ggggcgccag
     3601 gatcatggac ttcacggcgc cgggaaagct cgcagccgcg tacattccgg cgcgcagatt
     3661 ctttgttggt ggcaggtcca gcttgaccag gaagctgtgg cttaccggat cggcggcggg
     3721 cacaatctcg gcgaccgtac cgttaaggtc cgcgtcatgg attcccccgg cgctcaccgg
     3781 cactttcatg ccgatgtgca cagcgccgat gattgactcg tcgacggtgg tgaagacctg
     3841 cagggggccc tcgcgatcca gctgcagcag cggtacaccc gggacagcga gcgttccagg
     3901 atccgccagg cgtgccgtga tcacaccggc gaacggcgca tgcagcgtgg cgtagccgag
     3961 ctgggtccgc gcaccttcga ctgcagcctg ggctccccgg ctctgtgctt ccagtgcctg
     4021 caactgcagg gtcgcgccct gggaccgctt ctctacctca tcgaattcct gcgggctgac
     4081 gctcttctgt gctttcagaa tctcgtaacg cgccagggtt ccggcggcca gctgcgcgtt
     4141 ggcctgcgca gcggcccgct gcttctccag cgccgtttgc gcggcggtcg tctggccgag
     4201 cgccgactgc atcgcggcat cgtcgagcgt cgccagcagt tgtccggcac gcacatggcc
     4261 cccagcctgg acctgcacgc tgcgaagacg ccccggcacc tgggcggaga tcatcgctgt
     4321 ctcctttgcg tgaaccgttc ccgtcatctg gatcgtctgc gccgtgttgg cggcggcgct
     4381 gtgcaccagc tgcgcctgcg cagtgggcgg cgcggccgga acgggggcgg aacgcgagca
     4441 acccagcgcg atccaggctg aaacgatcgc cgaagcaagg tacaaagagc gtttcattgc
     4501 agagtctccg cggtgtcagg agtcagttgt ccggtggcgt agagcagagc tgcccaggcc
     4561 accgtgtttc cataagcggc atgccagtaa ttgttctggc tctgccgctc ggcatcctcg
     4621 gcatggagca gatcggtcat cgttgccaat ccggcatcgt aacggttgtg caggatgcgc
     4681 aaaccctcag cggcctgttc catcgcagcg cgagaggcgg cgaccatgcg ctccgtggtc
     4741 tggtgtcctg taaacgcgcg gctcacagcc aggcgaatct gctgttcctg actgcgctcc
     4801 tcggccgcgg ccttcgcctg agcggcgcgt gcctcggcaa aatgcgcacg cttcgagagc
     4861 ggcaggatat cgagagtgag ctgtaccccg gcgacccagt tgttgccgcc gtcaccggca
     4921 aaggtcgggc ggtccgtttc ccagttgccc gaggcgctca gctgcggaaa gaaatccgat
     4981 cgcgccgcct tcgccgcctg ttcctgcgca gcggtctgtt tccgcagggc ggtcagatcc
     5041 gggcgcgccg caagcgccct ggcgaggtct tcctcgaacg cgccgccgga gaaagttttt
     5101 gcgtcgattg gttgtagttg cggccgcggg ttcacggcac cacccatcgc agtttccagc
     5161 tccgcccatg ccgtctccac ggacccttgg gcagcaatgt tctcctgctg ccgcgcggcg
     5221 aggttcacct gcgccgacag gagatcggaa tcgatggcta atcccgcctt cacccgcgtg
     5281 ccagcatcac gaaccagggc ttcggcggtg tcctgctgat gctgtgcgag gtcgagttgc
     5341 cgctgtgcgt gcaagacagc ctggtaggcc tgcaccacct ggaagacgat gccctgatcg
     5401 gtggccttct ccaccgcggc cgcgctctcg gctcccagcc gggcagcatg gatctgttgc
     5461 tgcgtctcga atgcgttgaa gatcatccac tggccggtga atcgcgtggc gaagttgccg
     5521 gtcggc
//
LOCUS       DASZYN010000398        17096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_980530, whole genome shotgun sequence.
ACCESSION   DASZYN010000398 DASZYN010000000
VERSION     DASZYN010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 17096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17096
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980530"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2767)
                     /locus_tag="VHE33_10215"
     CDS             complement(<1..2767)
                     /locus_tag="VHE33_10215"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HVT97873.1"
                     /translation="MSHPSPLSADKQTLLALRQLRQRVEELETAAREPIAIVGMACRF
                     PGGASSPEDFWNLLEGKRDAIKEIPRWRIDLEPIFTPYPAQAGRTYSRWAGLLDRPDA
                     FDAEFFGIAPREAAAMDPQQRLLLEVSWEALEDAGINPKNLAGADAGVFLGISASEYG
                     QCAQRSLPSEQLSAYTLQGTALNAAAGRLAYFYGFSGPAVAIDTACSSSLVAVDRACR
                     SLRDGESCVALAAGVNLVASAEGFIIASQWGMLSPRGICAAFDDEADGFVRGEGCGVV
                     VLKRLRDAEAAGDRVLAVILGSTVNQDGASSGLTVPNGLAQQALLREAHRRAGIEARQ
                     VGYIEAHGTGTKLGDPIEAEALGTVFSGRQNKLAIGSVKSNIGHLESAAGIAGLIKLV
                     LSLEHGKIPGQVHWRRPSSHVPWQELPLEVIPESHAWEPIEGRRIGGVSSFGFSGTNA
                     HVVVEGRSREATSETGEGEDVLVISARNEAALRELAERYTGFLETEAEWSWAEICHTA
                     GMGRAAMSERLAVVAGNRTEAAGKLRAWLRGDMIGGISSGHISAGQRGAPHVDASATP
                     WQISEAWAQGAKPDWKQRCGARRLRRAQLPKYPFQRERYWIEHSKETRGIGDATGRGM
                     LGRRLRVAGVRAQYETRLQPDGWIGEHEVEGEAVLPATGHIELMLEAGADTLGTACVL
                     EELSLQTRLEIAEDRRVQVTVEEETAGRSRVRIYAEGTERNWERVAEAWLRPAEKSKS
                     EGEDVDALRRRLRQRAGREAFYSGMATRGIHFGERFRGVDGLWSGEGEALGEIALTLA
                     TDETEWQSSPWWLDACLQVVGAAAGGEGMYLPASAERVELYGPPGERSWSHVRTRWLD
                     DRTIAADVTVLRVDGSLLARLSGFRFRKLTPSAPQTEICTLEWKQVELQEKTRLNGNW
                     LILCDQEQ"
     gene            complement(2764..6459)
                     /locus_tag="VHE33_10220"
     CDS             complement(2764..6459)
                     /locus_tag="VHE33_10220"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT97874.1"
                     /translation="MTSSHNDELLDHARKIIRDLRTRLAAAEAASLREPIAIVGMAFQ
                     FPGAGSDPEKLWKMFAEGRDAVGPVPADRWSGADYFASDPASPGKINTAQGAFLDNVR
                     RFDAAFFDITPREAVRMDPQQRLFLETAWHALEDAGLTKATITGTDTSVFVGVHNLSS
                     DYQGMQFADVETLDAWSATGTAHDMIAGRLAYWLDLHGPAVVVNTACSSSLAAVHLAC
                     RSLRAGDCTAAVVAGVNLLLSPGSTIAASQLQLLSPDGHCRSFDARAQGMGRGEGCGV
                     VVLKKLSTAQQGGDRILAVIRGSAMNQDGRTNGLTAPNGLAQQRVIQRALADAGVQPA
                     DVGYIETHGTGTPLGDPIEVEAIAAIFKGTRRAPCTLGAVKANLAHLEGAAGIAGLIK
                     TVMVLRRRWLPPVANLQELNPHLAVEGSGLEIPREGRAWPTNQPRIAGISSFGFSGTN
                     VHVVVEEASAVAQSASQSGPWPIALSAQTDEALRQLAERFANRLDSADPKELRAISYT
                     STIRRTAHPFRMAVQGNEPGEIAHLLRQRLAAPDLHSRSHGTQTDEHLMLWEKGGDVD
                     WKIFFPEPSEVVDLPLYPFQGREYWIEGASAAQAVGPAARNLSLRAQPGSAAPSDWFY
                     SVDFVEKDLEPRSSSHATERPLWILLGRESELRRQIAAAIRSRGEELVEDFANQPGIS
                     VQRPMAEMLSHCIGKGRRFVVCFPQQDELSELISKSLSVVQELLSCEGSAQLWFVMEP
                     RQQLANPDASLSSSLKAFARVVALEHPDRMGGVITAGAGSAVDVLHEIEAGDGEDNVL
                     LRDGRRWVSRLCRDNLRPGAPLQLREDRSYLITGAFGGIGIHVAEWLLAAGARHLVLA
                     GRRDPSVMGKPHMLVRLDEWRADGIDVRTCVCDVADETQVAKLFAEMDARGLPAAGVI
                     HAAAHVRFSPAEQASQEDVHDVFRVKLEGARVLDRCTRERALDFFVLFSSAAATLGLR
                     NGAIYAAANSSLQSVVEARCALGLPAVCVEWGSWESDHTGPQQALIEKSGFVAMQPQA
                     ALSGLAALLREGRSRGIVAHVDWEILGPALALRGRDALVSSVLREADEKLRAKDDAGS
                     AAWVDALHSLPAAEQQNRLLDLVAAEARGIFAMRPEDPLQEDRGLFAMGMDSLMAVRL
                     KRRLEERTGLRLPGTMTLTYPTLAALAAFLHTRLFGHEPGIVPSAARTETRDDLTKLI
                     AMDDDQTRAAIEEELAAVQQTLAKELR"
     gene            complement(6456..12842)
                     /locus_tag="VHE33_10225"
     CDS             complement(6456..12842)
                     /locus_tag="VHE33_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HVT97875.1"
                     /translation="MTIDNRNEPIAIVGMSCRFPGASDPEALWNLVRDRGESITEYPG
                     GRTPELDAFYQRAGMPDGPPSVRGGFLSDVDRFDAAFFEISPREAEWLDPQQRLLLET
                     GWEALEDAGIPLANVEHAGVFVGVWSNDYEKHATDHAPRVEFFNVTGNFLFGDSSRVA
                     FQFNMRGPDVSVNAACSSSIAAIHLAVRSLRSGECSLALAGGANVIVRPETTQAFSRV
                     GVLSPEGRCKFGDAAADGYVRSDGVGVLVLKRLSDALRDRDRVLALIRGTAVTNSGRT
                     SGTLSRPSADGQKRAMRAALADAQLDGAMIDFVEAHGTGTRTGDVLEVEAIIDVFGRA
                     ENRTSPCRTASVKSNIGHAESAAGISAIIRTVQAMHHHRFPASLHVKELNPRIDWDAG
                     VILEREGCEWTTHEGRPRRAGVNGLGLTGTNTHVILEEAPPYERQRTPNQAAFLLPVS
                     AASAAALRRRAADCAQAIERLETPGDSFADLVYTAARRRTHLPHRLAVVGSDAGEIRK
                     QLVAFAAGDEPAFLAAGNADAPRKVAFVFPGQGSQWVGMGRDLLRTSAAFRSTIETLD
                     PLIAEETGWSVLKQLEDPSLEERLSRIDVVQPTLFAMEVALADWWKSCGVHPDAVVGH
                     SMGEVAAACVAGILSPRDAVQVICRRSALLLRVAGKGAMAVVELTRDEAEQAIAGEEG
                     KISVAVCNSERSTVLAGDPEALDKIVNRLESRDVFCRWVRVDVASHSPHVDVLRDDLL
                     LQLSTVNPHSGSVPLYSTVRASLVDGARMDSRYWVDNLRNPVLFADAIGDLMRQGFNT
                     FVEMSPHPILVPFVEQTAAQIGTNALVVGSLRREEPETATALLSLGRLFAAGAEVDWK
                     TLFPEGNVVSLPAYPWQRERFWMEGALQTRRSGGHPLLGEPVTTASGERIWSGTVSVE
                     THPWLRDHQVGGTVLVPASAWVEMAIAAGKGLFGSAAVVEQIALTEALVVSSGTAVDM
                     QMIALPETSESRALQFFGREQGTVTWQQTGECRIRRGSAETFSFSDVAQWQDAELAAH
                     TITGTLHSQRMGELGYDFGPAFCCVDWMLRQDHTVLGQIRADQPRGEYWLHPATLDGA
                     LQVLAHLLIDVSGTRELLLPVAFERLEMHTADPPASVTRLLVRATADSAALRGTITLF
                     DSTGLALLTIHGAEMRPLERHENPADALYALEWHRLPPMTSSSPVIQRWILIGGRDET
                     ANELSRALQLKNAHTVRTTPDAILNATLPIGGAGAVWLAPLDLPVSTSLADIEKVLAE
                     GAALTAALAEQGVARIWLLTRGTQAVAGESVENVMTAGAWGMFAAVAHEYPSIPIGCI
                     DLPANPPERAMETVAAALLQNREETRVAVRGDSLFGAELHPWASSESERLRPGDLRPD
                     EAFEIQQSVPGSLSSLELRTIPADPPSGDEVEIAVEAAGLNFLDVLRAMGLNEALSSD
                     HFGGECAGTVVRVGEKVSTCRPGDSVLAISPSFRHTGMLASHVRVPEMLVARKPQGMT
                     FAEAAGIPCVFLTAWYGLVKLARLAKDEKILIHAAAGGVGLAAIQVAQWIGAEVWATA
                     GSDEKREYLRQLGVCHVMDSRSLDFSRQILAETNGRGVDVVLNSLAGPAIPASLDALA
                     PYGRFVEIGKRDLWENSRLGMRPFLRNLSYFAVDLADAVEARRAMVGELLRELMDHFA
                     VGDFRPSPTTVFPVSVAGDAFQHLARAAHIGKVVLSMNDPKTEIRIKSDRLHADASYL
                     VTGGLGGVGLAIADAMVEQGARHLVLTSRSAPSAAATAAISRMQDRGVQVEIRQTDMS
                     DATAVMALLARLVSAPQPLRGIVHAAGVLDDAVAANLSREKFHRVMAGKVAGALAIDA
                     SLKPGQLDFLVYTSSAAGVLGTAGQGNYAAANAMLDALAHRQRSRKIPAVSIDWGTWS
                     EVGLAAAALNRGARLESQGLIPLRPAFGQALFTRILAGMPIQAAAMIFDADRWCAASP
                     ASARSGFLNSLRRSSDNVSRTDSDQLAEFASIQGDELRNRLVAWLRQQVAAVLRLDVE
                     RVPEDKPVRTLGLDSLMALELRNRLERHLHTKLSATLVWNYPTVAKLAEFLQKRLEEK
                     RQKSGPEERRPQLAELPAVEEAVLAQVGVLSAAEMLEAELLEAESLLNAQAGAP"
     gene            complement(12839..13606)
                     /locus_tag="VHE33_10230"
     CDS             complement(12839..13606)
                     /locus_tag="VHE33_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HVT97876.1"
                     /translation="MFQRPPSRNDSVIRLFCVPHAGSGATAFRGWAQPLLPEIEATVV
                     ELPGRESRFRDMPYPRMEPLVNDLASAVIAALAEDQPFAFFGNSLGSLIAFETLHEIH
                     RRTGREALHLFVSASGAPQCEPPLPPIGHLADRELVRAVDERYGGIPAVVLADKEFLA
                     AILPTLRADICLLEAYQRPEPKPLTCSVTAFAGLRDATVPLEHVEAWRDQTTGSFERV
                     CLNEGHLYLQTARDLLVHRVRDNLLAGAPMRQEEISQ"
     gene            complement(13637..14452)
                     /locus_tag="VHE33_10235"
     CDS             complement(13637..14452)
                     /locus_tag="VHE33_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT97877.1"
                     /translation="MSTFLSKAGAFIRRDFRIESGYQAAFLMGLLESIMLLVILHFVG
                     QLIPASGSAGLGKYGRGYFPFALVGVAFARYFDLMLRMFSESIRTAQVTGCLEAMLSS
                     QTGPITVVTMSSFYSLLSGAVQLLLILLGGVFLFGVDLRAMNVPATLLVLLFSIAIFV
                     AFGVLSASAIVWLKKGDPITWLLGGMGSILGGAYFPIDVMPGWMQKISYVIPIRYSLD
                     ALRSTMLRGASVVSVARPIETLLVMAVILLPASAAVFTATVRRGRREGTLAQY"
     gene            complement(14449..15309)
                     /locus_tag="VHE33_10240"
     CDS             complement(14449..15309)
                     /locus_tag="VHE33_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT97878.1"
                     /translation="MSSLKSIVMEKPAVVESPVQEPSSAPAIAVRDVTMRFPIPKRYR
                     EWVTHPLRARRVVTALQNASVDIQAGDRIAVMGPNGAGKTTLLKLISGLLFPTEGEVT
                     VNGLSTLHDNAAARKSVGFVFNEERSFYWRLTGVQNLEFFGALDNLRGQALKERIREL
                     MQLVGLERAGDQLVSGYSSGMKQRLALARVLLSDPEVLILDEPTRALDPVAADEMVNL
                     VLTDIHRGSRKTLLIATHRPEEAVTLCSKVMVIDGGRMRGFVNLAHLKEEGTTLLDYY
                     RRCVAGGSAA"
     gene            complement(15306..>17096)
                     /locus_tag="VHE33_10245"
     CDS             complement(15306..>17096)
                     /locus_tag="VHE33_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVT97879.1"
                     /translation="EVWNLYGPTETTIWSTVDRVQNDSKVTIGRPIANTDIYVLDEHG
                     APVPPGVVGELHIGGQGLARGYRNREEENRARFIQSERYRARLYRTGDLARRLSDGRF
                     ECMGRADHQVKLRGYRIELGEIEAALQQRSEIQQAVAVVREDVASDPRLVAYLTTQNG
                     SVPDAAALRATLKKSLPEYMIPSLFLELGAFPLTANRKVDRKALMGPEFAPEPKVAGS
                     DGIGAAKGTVHGLSAEQPRNHVEAVMAEVWREVLHLDQFGIFDDFFVLGGHSLSAASV
                     VARLRTELEMEIPLRSVFLDPTIAGLASHIAYDATQGGYRYSSEIPEWNCLVPVQPRG
                     SRTPFFFITGYQNPDDTLQFLTPLIQYFGKDQPVFGFRPRWTFGGADYESVGEMAREF
                     LKELRAVQPHGPYLLGGMCVGGIAALELARTLMEQGEQIQLMLLVDADRPGPDTARAA
                     ERNYILRRIRHIRGVLAEIAHADKRQKVQMVRTLFRRKMGIEDRPEILEQDRYYNSKV
                     RYWRMLYAHCPQKYAGRLVLIVNEEQHRFNSDLGWTGFADGGLEILTSPGSHETMFTD
                     HRKAAAERILRCIKKATEADQAEQPAEALR"
BASE COUNT         3399 a         5566 c         4965 g         3166 t
ORIGIN      
        1 actgttcctg atcgcacagg atcagccagt tgccattcag tcgggtcttc tcctgaagct
       61 caacttgttt ccattcgagc gtgcagattt ctgtctgtgg cgctgacgga gtgagtttgc
      121 gaaagcgaaa gccggagagc cgggcgagca gggagccgtc gacgcgaagg accgtcacat
      181 ccgcggcgat agtgcgatca tccagccagc gtgtgcgcac atggctccag ctgcgctcac
      241 cgggaggtcc gtacaactcc acgcgctcgg cactcgccgg cagatacatg ccttcgccgc
      301 cggccgctgc gcccaccacc tggagacacg catccagcca ccagggcgat gactgccatt
      361 cggtttcatc ggttgctaac gtcagggcaa tttcccccag cgcctctccc tcaccactcc
      421 aaagcccgtc cactccgcgg aaccgctctc caaaatggat tccccgcgtt gccatcccgg
      481 aataaaaggc ctctctgccg gccctttgcc gcagccgacg ccgcaatgca tcgacatctt
      541 ccccttcgct tttggatttc tctgccggcc gcagccatgc ctctgcaacg cgctcccaat
      601 tcctctcggt gccttcggcg tagatccgca cacggcttcg acccgccgtc tcctcctcta
      661 ctgtcacctg tacacgccgg tcttccgcga tttccagccg agtctgcagc gaaagttctt
      721 cgaggacaca cgccgtgccc agcgtgtcgg cgcctgcttc cagcatcagc tcaatatgcc
      781 cggtggcagg caatactgct tcaccctcga cctcatgctc gccgatccag ccgtcaggct
      841 ggaggcgggt ttcgtactga gcccgcactc cggccacacg caggcgcctt cccagcattc
      901 cccgtcccgt agcgtctccg attccccgcg tctctttgct gtgctcgatc caataccgct
      961 cacgctgaaa tggatacttc ggcaactgcg cgcgacgcag acgacgcgcg ccacaccgct
     1021 gcttccagtc cggcttcgct ccctgcgccc acgcctcgga gatttgccac ggtgttgccg
     1081 aggcatcgac atgaggcgct ccgcgctggc cagcactgat gtggcctgaa ctgatgcctc
     1141 cgatcatgtc gccacgcagc cacgctcgca gcttccctgc tgcctccgta cgattgccgg
     1201 ccacaaccgc cagccgttca ctcatcgctg cacgacccat tccagccgtg tggcagatct
     1261 ccgcccagct ccactctgct tctgtttcca ggaatcccgt gtatcgctcg gccagttctc
     1321 gcaacgccgc ttcgttccgg gcggagatca ccaaaacatc ttcgccttcg ccagtttcgc
     1381 ttgttgcttc ccggctccgg ccttccacca ccacgtgcgc gttggttccg ctgaatccga
     1441 acgaacttac gcctccgata cgccgtcctt cgatcggctc ccaggcatga ctttccggaa
     1501 tcacttcgag cggcaattcc tgccagggca catggctgct gggccgccgc cagtgcacct
     1561 gtccgggtat cttgccgtgc tcgagactca acaccagctt gatcagcccc gcaatgccgg
     1621 ccgccgactc cagatggccg atgttggact ttaccgatcc gattgccagc ttgttctgac
     1681 ggccggagaa gaccgtgccc agcgcctctg cctcgatcgg atcgcccagc ttcgtcccgg
     1741 ttccgtgcgc ttcaatgtag cccacctgtc gcgcttcgat tcccgcacga cgatgcgctt
     1801 cccgcaataa cgcctgttgc gcgaggccat tcggcacggt caatccgctg gaggcgccgt
     1861 cctgattcac cgtcgaaccg agaatcacag ccagcacgcg atctccggcc gcctctgcgt
     1921 cgcgaaggcg tttcagcacc accacgccgc acccttctcc acgtacaaat ccatcggcct
     1981 cgtcgtcaaa ggccgcacag atgccgcgcg gagacagcat gccccattgc gaagcgatga
     2041 tgaatccctc tgcgctcgca accagattca ctccagcagc aagagccacg cacgactcac
     2101 catcgcgcaa actacgacag gcccgatcga cggccaccag tgaggaggaa caggctgtat
     2161 cgatggcaac agccgggcca ctgaatccat aaaagtaggc gagccgtcct gcagctgcat
     2221 tgagtgccgt tccctggagc gtgtaagccg acaattgctc ggacggaaga gagcgttgtg
     2281 cgcactggcc gtactctgaa gcgctgattc cgaggaagac tccggcatcg gcgccagcga
     2341 gattctttgg attgatgcct gcgtcttcca gggcctccca gctcacttcc agcaacagcc
     2401 gctgctgcgg atccatggcc gcagcctcgc gcggtgcgat tccgaagaat tcggcatcga
     2461 acgcgtcggg gcgatccagc aggcctgccc atcggctgta ggttcggcca gcttgcgcgg
     2521 gatacggtgt gaagatcggc tccagatcga tacgccaccg gggaatttct ttgattgcgt
     2581 cccgttttcc ttcgagcaga ttccagaagt cttccggact ggaggcaccg ccggggaaac
     2641 ggcaagccat gccgacgatg gcaatcggct cgcgcgcggc ggtttccagt tcctccacgc
     2701 gctgccgcag ctggcggagg gcgagaagtg tctgcttgtc tgcggacaag ggcgatggat
     2761 gactcatctc agctccttcg ccagagtctg ctgcacggca gccagctctt cctcgatggc
     2821 tgcacgcgtc tggtcgtcgt ccatcgcgat caacttcgtc agatcatccc tggtttcagt
     2881 tcgcgcagcg gacggaacga ttccgggttc atgaccaaaa agccgggtgt gcagaaatgc
     2941 cgcaagggca gccagcgtcg ggtaggtcag cgtcatcgtg ccaggcagcc gaagtccggt
     3001 tctttcttcc agacgacgct tgagccgcac ggccatgagg gaatccatcc ccatagcaaa
     3061 gagaccacgg tcttcctgca gcgggtcctc cggccgcatg gcgaaaattc cgcgagcctc
     3121 tgcggcgacg agatcaagaa gacggttttg ctgctcggcg gccggtaacg aatgcaacgc
     3181 gtcgacccag gcagccgaac cggcatcgtc ttttgcacgc agtttctcat cggcttcacg
     3241 gaggacggaa gagacgagtg cgtcccgacc tcgcagggcc agtgccggcc cgaggatctc
     3301 ccagtccacg tgggcgacta tgcctcggct tcgcccttcg cgcaggagcg ctgccagccc
     3361 cgaaagagca gcctgcggct gcatagcgac gaaaccagat ttctcgatga gggcttgttg
     3421 tgggcctgtg tggtccgact cccaggagcc ccattcaaca caaactgctg gcagtcccag
     3481 ggcgcatctc gcttccacca cactttgcag cgagctgttg gcggcagcgt agattgctcc
     3541 gttacggaga cccaaagtgg ccgcggccga gctgaacagc acaaagaagt caagcgcccg
     3601 ctcgcgggta cagcgatcga ggacacgcgc gccctccagc ttgacgcgaa agacatcatg
     3661 cacatcttcc tgcgacgctt gttctgcggg cgaaaagcgc acatgggccg cggcatgaat
     3721 cacacccgcc gcgggcaggc cgcgcgcatc catctcagca aacaatttcg ccacctgcgt
     3781 ctcgtccgcg acgtcgcaga cacaggttcg tacgtcgatt ccgtctgcgc gccattcatc
     3841 aaggcgcacc agcatgtgcg gctttcccat caccgatggg tcgcgtctgc ctgcgaggac
     3901 caggtgccgc gcacccgcgg cgagcagcca ctcggctacg tgtatgccga tgccaccgaa
     3961 ggcacctgtg atcaggtagc tgcggtcctc gcgcagctgc aaaggcgcac caggacgcag
     4021 attgtcgcga cacaggcggg acacccagcg acggccatcg cgcagcagaa cattgtcctc
     4081 gccgtcgcct gcctcgatct catggagaac atcgacagcg cttccggcac ccgcagtgat
     4141 gacgccaccc attcgatccg ggtgctcgag tgcgacgact cgtgcaaatg ccttcaggga
     4201 tgaggacagc gacgcgtcgg gatttgccag ttgctgtcgc ggctccatca caaaccatag
     4261 ttgcgccgat ccttcgcacg aaagaagctc ctgcaccacg ctgagagact tgcttatcag
     4321 ctccgagagt tcatcctgct gcggaaaaca gactacgaag cgccgacctt ttccgatgca
     4381 atgggaaagc atttctgcca ttggcctttg cacggagatt ccgggctgat ttgcaaagtc
     4441 ctcgaccagt tcctctccac ggctccgaat tgcagcagcg atctgacgtc tgagctccga
     4501 ttcgcgcccc agcaggatcc aaagagggcg ctccgtggcg tgcgacgacg agcgtggctc
     4561 cagatctttc tccacaaagt ccaccgagta gaaccagtcg ctgggagcag cggagcccgg
     4621 ttgcgcacgt aaagacaaat tcctggcagc cggacccacg gcttgtgcgg cggacgctcc
     4681 ctcaatccag tattccctgc cctggaacgg gtaaagcggg aggtcgacga cttcggaggg
     4741 ttcggggaag aagatcttcc agtcgacatc gccacccttc tcccaaagca ttaaatgctc
     4801 gtccgtttgc gtgccgtgcg accgactgtg caagtccggt gcggcgagcc gctgccgaag
     4861 caggtgtgca atctccccgg gctcgttgcc ctgaaccgcc atgcgaaacg gatgagcggt
     4921 ccgtcgaatc gtgctggtgt aactgatcgc gcgcagttct tttggatccg cagagtcgag
     4981 gcgattggca aaccgctccg ccagctgacg caaggcctca tctgtctgtg ctgaaagtgc
     5041 gatcggccat gggcctgatt gacttgctga ttgcgctact gcggacgctt cttcaaccac
     5101 tacatgaaca tttgtgccgc tgaatccaaa tgagctgata ccggcgatgc gcggttgatt
     5161 cgtcggccag gcgcgccctt cacgaggaat ctcgagtccg gagccctcca cagccaggtg
     5221 cggattcagt tcctgcaggt ttgccaccgg aggcagccag cggcggcgca gaaccatgac
     5281 ggtcttaatg agtcctgcga taccagcagc tccttcgaga tgagccaggt ttgccttgac
     5341 ggcccccagg gtgcacggag cccgacgtgt tcctttgaaa atcgccgcga tcgcctcaac
     5401 ttcaatcggg tcgccgagag gcgtgccggt gccgtgtgtc tcgatgtagc ccacgtcggc
     5461 aggctgaaca ccggcatcgg ccagggcacg ctggatcaca cgctgctgtg ccaggccatt
     5521 gggcgcggtg aggccgttgg tgcggccgtc ctgattcatt gcggagccgc gaatcactgc
     5581 gagaatgcga tcaccgccct gttgagctgt tgaaagcttc ttcagcacga cgacgccgca
     5641 accctcgccc cgacccatgc cctgagcgcg cgcatcgaaa ctgcggcaat gaccgtcggg
     5701 cgagaggagc tggagctgcg aagccgcgat ggtcgaacct ggcgacagca acaaattgac
     5761 tccggcgacc acagccgcgg tgcagtcgcc ggcacgcagg ctgcgacagg ccagatggac
     5821 agcagccagc gaagatgagc aggctgtgtt gacgaccaca gccggaccat gcaggtcgag
     5881 ccagtaggca aggcgaccgg cgatcatatc gtgtgctgtg ccggttgccg accacgcatc
     5941 cagggtctcc acatccgcaa actgcatgcc ctggtagtcg ctgctgaggt tgtggacgcc
     6001 aacgaagacg ctggtgtctg tacccgtgat cgttgccttc gttaaacccg catcttccag
     6061 cgcgtgccag gctgtttcga ggaaaagtct ttgctgcgga tccatgcgca cggcttcgcg
     6121 cggagtgatg tcgaagaagg cggcatcgaa gcggcggacg ttatccaaaa acgcgccctg
     6181 tgcggtattg atctttcctg gtgaggcggg atcggaagcg aagtagtccg ctccgctcca
     6241 gcgatcggca ggaacaggac cgaccgcatc gcgaccttcg gcgaacatct tccacaattt
     6301 ctccggatcg gagcccgcgc caggaaactg gaaggccatg ccaacaatgg caatgggctc
     6361 acgtaggctc gccgcctcgg cggcagccag tcgcgtccgg agatcacgga tgattttgcg
     6421 agcgtgatcc agaagctcat cgttgtgact ggaggtcatg gcgcccctgc ctgcgcattc
     6481 agtagcgatt ccgcctccag caattcggcc tcaagcatct ctgcggcaga gaggactccg
     6541 acctgcgcga gcacggcttc ctctacagca ggaagctcag cgagctgtgg ccgacgttcc
     6601 tccggcccgc tcttctggcg tttctcctcc agccttttct ggagaaattc cgcgagcttg
     6661 gcgacggttg ggtagttcca caccagagtc gcggagagct tggtgtgcag atggcgttcg
     6721 agccggttgc gcagttccag agccatcaga gaatcgaggc cgagagtgcg aacgggcttg
     6781 tcttcaggaa cgcgctcgac atccagacgc aggaccgctg caacctgttg acgcagccac
     6841 gcgacgaggc gattgcgcaa ctcgtcgccc tggatcgaag caaattctgc cagctggtca
     6901 ctgtctgtgc gggacacgtt gtcgctggag cgtcgcagtg aattcagaaa gccggaacga
     6961 gccgatgccg gggacgcggc acaccagcga tccgcatcga aaatcatggc cgcggcctga
     7021 atgggcattc cggcaaggat ccgggtgaaa agcgcctgcc caaacgctgg acgcagaggg
     7081 atcaagccct ggctttccaa acgggcgccg cggttcaggg ccgccgctgc gagtccaacc
     7141 tcgctccatg tgccccagtc gatgctcaca gcgggaatct ttcgcgagcg ctggcgatgg
     7201 gccagagcgt ccagcatcgc attcgcggcc gcatagttgc cttgtccggc ggttcccagc
     7261 acgccggcag cagaggacgt gtagacgagg aaatccagtt ggcccggttt gagcgatgca
     7321 tcgatcgcca gggcaccagc tactttccca gccatcacac ggtggaactt ctcccgagag
     7381 agattcgctg ctaccgcatc gtcgaggact ccggccgcat gcacaatccc gcgaaggggc
     7441 tgcggagcgg acaccaatcg ggcgagcaga gccatcacgg cggttgcatc gctcatatct
     7501 gtctggcgga tttcgacctg cacaccacga tcctgcatcc gagaaattgc cgctgtcgca
     7561 gcagctgatg gcgcacttct cgacgtcagc accagatgcc tggctccctg ctccaccatc
     7621 gcatcggcga tcgcgagacc gactccgccg agaccacctg taaccaggta gctggcgtca
     7681 gcgtgcaggc gatccgactt gatgcgaatt tcggttttgg ggtcgttcat ggacaggacg
     7741 actttgccga tatgtgcggc gcgggccaga tgctggaatg catcgccagc gacggagacc
     7801 ggaaagacgg tggtcggcga gggacggaaa tcaccaacag caaaatgatc catcagctcg
     7861 cggagcagtt cgcccaccat ggcgcgacgt gcttcgacgg catccgccaa atccacggca
     7921 aagtaggaaa gattcctgag gaacggccgc atgccgagcc gactgttctc ccacagatcg
     7981 cgcttgccga tttccacgaa gcgtccatag ggggccagag cgtcgagact ggccggaatc
     8041 gcaggccccg ccagggagtt cagcacgaca tcgacgccgc ggccgtttgt ctcggcgaga
     8101 atctgccggg aaaaatccag cgaccgcgaa tccatcacgt ggcacacgcc gagctgccgt
     8161 aaatattctc gcttctcgtc ggatccagcg gtggcccaga cctctgcgcc aatccattgc
     8221 gcgacctgaa tggccgccaa accaacacca cccgcggcag catggatcag gatcttctca
     8281 tctttcgcga ggcgagcgag cttcaccaat ccgtaccagg cggtcaaaaa cacgcacggg
     8341 atgcctgctg cttcagcaaa cgtcatcccc tggggcttgc gcgcaaccag catctccggc
     8401 acacgcacgt gcgaggcgag cattccggta tgccggaaag acggagagat ggcgaggacc
     8461 gaatcaccag gccggcaggt gctgactttc tctcccactc gcacaacggt gccggcacac
     8521 tctccgccga agtgatcgct ggacagggcc tcattgaggc ccatggctcg cagaacatcg
     8581 aggaagttca gcccggcagc ctccacggcg atttctactt catctccgga tggcggatcc
     8641 gcgggaatgg tgcgcaactc aaggctcgac agcgaaccgg gcacactctg ctgaatttcg
     8701 aaggcctcgt cggggcgcaa atctcctgga cgcagacgct cactttctga ggaagcccag
     8761 ggatgcagtt cagcgccgaa caggctatcg ccgcgaacag caactcgggt ttcttcacgg
     8821 ttctgcagca aagccgcggc cactgtttcc atcgcgcgct ccggcggatt cgccggcaga
     8881 tcgatgcagc cgatcggaat cgacggatat tcatgggcca ccgcagcaaa catgccccat
     8941 gctcccgccg tcataacgtt ctcgacggat tcccctgcaa ccgcctgggt gccgcgcgtc
     9001 agcagccaaa tccgggcaac tccctgttcc gcgagtgcgg cggtgagtgc cgccccttcg
     9061 gccaggactt tctcgatatc cgcgagcgat gtgctgaccg gcaaatccag aggcgccagc
     9121 cacactgcgc ctgcgccacc gatgggcagc gtggcgttca ggattgcatc cggcgtggtg
     9181 cggacggtat gagcattctt cagttgaagg gcgcggctga gttcgtttgc ggtctcatct
     9241 cggccaccaa tgagaatcca ccgctgaatg acaggagaag acgaagtcat cggaggcagt
     9301 ctgtgccact cgagcgcata tagagcgtca gctgggtttt cgtgacgctc cagggggcgc
     9361 atctccgcac cgtgaatcgt gagcagagct aaaccggtgg aatcgaagag cgttatggtt
     9421 ccacgtaagg cggctgaatc ggcagtcgct cgcaccagca gccgtgtgac gctcgcggga
     9481 ggatcggcag tgtgcatttc cagtcgttcg aatgcaaccg gcaaaagcag ctcacgggtt
     9541 ccgctgacat cgattaagag gtgagccagg acctgaagcg caccatcgag tgtggccgga
     9601 tggagccagt attctccgcg gggctgatcg gctcgaatct gcccgagtac ggtgtgatcc
     9661 tggcggagca tccagtcaac gcagcaaaaa gccggcccga agtcgtatcc cagctcgccc
     9721 atacgctggc tgtgcagcgt ccctgtgatc gtgtgcgctg caagctctgc gtcttgccat
     9781 tgagcaacat cggaaaaaga gaatgtctca gccgaaccgc ggcgtatccg gcattcgccg
     9841 gtttgctgcc aggtgacagt tccctgctcg cggccgaaga actgcagcgc gcgggattcg
     9901 gacgtctcgg gcagggcgat catttgcata tcgaccgcgg ttccggacga cacaaccagc
     9961 gcctccgtca gagcgatctg ctcgacgacg gcggctgagc cgaagagccc ttttccggcg
    10021 gcaattgcca tttcgaccca ggcgctcgcc ggaaccagaa cggtcccgcc aacctggtga
    10081 tcgcgcagcc agggatgcgt ctcgacggaa accgtgcccg accagatgcg ttcgccggag
    10141 gccgttgtga ctggttcgcc cagcagcggg tgaccgccgg agcgccgcgt ttgcagcgcg
    10201 ccttccatcc aaaaacgttc gcgctgccac gggtaagcag ggagcgagac tacattgccc
    10261 tccggaaaga gagtcttcca gtcgacctcc gcaccagcgg caaacaatcg ccccagcgac
    10321 agcagggccg ttgccgtttc cggttcctca cgacgcagtg aaccgactac caaagcgttg
    10381 gtgccgattt gagccgccgt ctgctccaca aacggaacaa ggatcggatg cggactcatc
    10441 tcgacaaatg tgttgaagcc ctgacgcatg agatcgccga tggcgtcggc aaaaagaacc
    10501 gggtttcgca gattgtcgac ccagtagcgg ctgtccatgc gggcgccatc cactaaggag
    10561 gcgcgaacgg tcgagtagag cggaacggag cccgaatgcg gattgactgt cgacaattga
    10621 agaagaagat cgtcgcgcag gacatccacg tggggactgt gggaggccac atcgacccgc
    10681 acccagcggc agaatacatc gcggctttcc agcctattta cgatcttgtc gagagcttcg
    10741 ggatcgccgg caagaacggt cgagcgctcg ctgttacaga ccgcaacgga gatctttcct
    10801 tcctctccgg cgatggcttg ctcggcctcg tcgcgagtca actccacgac ggccatggcg
    10861 cccttacccg caacgcgcag caacagcgcg ctcctgcggc aaatcacctg gacggcatcg
    10921 cgaggagaga gtattccggc aacgcaggct gcggccacct cgcccatgct gtgtcctaca
    10981 actgcgtccg gatgcactcc gcaggatttc caccagtctg cgagcgcgac ttccatcgcg
    11041 aagagtgtcg gctgcacgac gtcgatgcgc gaaagccgtt cttccaaaga tgggtcttcc
    11101 agttgcttca aaacagacca gcctgtttct tcggcgatga gtgggtcgag tgtttcaatc
    11161 gtgctgcgaa aagccgcgct ggtgcgcagc aggtcccggc ccatgcccac ccactgcgaa
    11221 ccctgaccgg gaaagacgaa ggcgaccttt cgcggcgcat ctgcgttgcc tgcggcaaga
    11281 aacgcgggct cgtccccagc cgcgaaagcc acgagctgtt tacggatttc tccggcatcg
    11341 gaacccacca ctgccagccg gtgcggcaga tgggttcggc gccgcgctgc cgtatagacc
    11401 agatctgcga acgaatcgcc gggtgtctcc agcctctcga tcgcctgggc gcagtcagcc
    11461 gcacgcctgc ggagtgccgc tgcggaagct gccgaaaccg gcaaaagaaa ggctgcctgg
    11521 ttcggagttc tttgccgctc gtacggcgga gcctcctcga gaatcacgtg ggtgttggtt
    11581 ccggtcaggc cgagtccatt cacaccggca cgccgcggcc gcccctcgtg tgtggtccac
    11641 tcgcagccct cgcgttccag gatgactcca gcgtcccagt cgatccgggg attcaattcc
    11701 ttcacatgca ggctcgcggg aaaacggtgg tgatgcatcg cctgcacggt gcgaatgatg
    11761 gccgaaatgc cggccgccga ttcggcatgg ccgatgttcg atttcaccga cgcagttcgg
    11821 catggagagg tcctgttctc cgcgcgcccg aacacgtcga taatcgcttc gacttccagc
    11881 acatctccgg tacgcgtgcc ggtaccgtgc gcctcgacga agtcgatcat ggctccatcc
    11941 agctgcgcat ctgccagtgc cgcacgcatc gcccgttttt gtccgtccgc actcggcctc
    12001 gacagcgttc cgctggtcct gccactgttg gtcacggcgg ttccgcgaat cagagccaga
    12061 acccgatccc ggtcacgcag cgcgtcagag aggcgcttca ggacgagcac gccgacgccg
    12121 tcgctgcgca cgtatccgtc cgcggcggcg tccccaaact tgcatcttcc ctcgggcgaa
    12181 agcacaccaa cgcgagaaaa cgcctgcgtc gtctccggac gcacgatcac attggcgccc
    12241 ccggccagag ccagcgagca ctcgcccgag cgcagggacc gcaccgccag atgaatcgct
    12301 gcaatggaag aactacaggc tgcattcacc gacacgtccg gcccgcgcat attgaactgg
    12361 aaggcaacgc ggctggaatc cccaaacaga aaatttccgg tgacattaaa gaactccacc
    12421 cggggtgcat ggtcggtcgc atgcttttcg tagtcgttgg accacactcc gacgaaaaca
    12481 cccgcgtgct cgacattcgc gagaggaatg cccgcatctt ccagcgcctc ccacccggtt
    12541 tccagcagca aacgctgctg cggatcgagc cactcagctt cgcgtgggga gatctcgaaa
    12601 aaagcggcat caaagcgatc gacgtcggag agaaagccgc cgcgcaccga gggcggtccg
    12661 tccggcattc ctgcccgctg gtagaacgcg tccagttcag gagtcctgcc accgggatac
    12721 tccgtgatgc tctcgccgcg atcgcgaacc agattccaga gtgcttcggg atcggaggca
    12781 ccgggaaaac ggcaactcat gccgacaatc gcgatgggct cgttacgatt gtctatagtc
    12841 attgcgaaat ctcctcctgc cgcatcggag ccccagccag cagattgtcg cggacgcgat
    12901 ggacgagaag atcgcgggcg gtctgcagat acaggtggcc ttcgttgagg caaacccgct
    12961 cgaaggagcc ggtggtttgg tctcgccacg cctcaacgtg ttcgagtgga accgttgcgt
    13021 cgcgaagacc cgcgaaggca gtcacagagc acgtgagcgg tttcggctca ggacgttgat
    13081 acgcctccaa caggcagatg tccgcgcgca gggtgggcag aatcgccgcg aggaactctt
    13141 tgtcggccag cacgactgcc gggatgccgc catagcgctc atccacggca cgaaccagtt
    13201 cgcgatctgc taagtgccca atcggcggga gtggaggttc gcactgcggg gcgccggagg
    13261 cggagacaaa caggtgcaga gcctcgcggc cggtccggcg gtgtatctcg tggagcgtct
    13321 caaaagcgat gaggctgccc agactgttgc cgaagaatgc aaagggctga tcctcagcca
    13381 gggcggcgat cacagcgctg gccagatcgt tcaccagggg ctccattcgc gggtacggca
    13441 tgtcgcgaaa tctcgattcc cgtccgggta gttccaccac cgttgcttcg atttccggca
    13501 gcaggggctg agcccaaccg cgaaacgcag ttgcgccgga tcccgcatgg ggaacgcaga
    13561 aaagccgaat gactgaatca tttcgagatg gaggtcgctg gaacatcgag atcgtttgcc
    13621 ctgaggttac tgagttttag tattgcgcca acgtgccttc tcttctgcct ctgcggacgg
    13681 ttgcggtaaa tacagccgcg ctcgccggca ggagaatgac ggccatgacc agaagcgtct
    13741 cgatcggtcg tgccactgaa accacgctgg cgccccgcag catcgtcgaa cgcaacgcat
    13801 ccagcgaata ccgaatcggg atcacatagg agatcttctg catccatccc ggcatcacgt
    13861 cgatgggaaa ataggcgccg cccagaatgc tgcccatccc gcccagaagc caggtgatcg
    13921 gatcgccctt cttcagccag acgatcgctg acgccgacaa gaccccgaag gccacaaaaa
    13981 tagcgatcga aaacagcagc accagcaacg tcgccggcac attcatcgct cgcaggtcca
    14041 ctccgaacag gaacaccccc ccgagcaaaa tgagtaacaa ctgcacagcc ccggaaagca
    14101 ggctgtaaaa cgaggacatg gtcaccacgg tgatcggccc tgtctggctt gacagcatcg
    14161 cttccagaca accggtcacc tgcgccgtgc ggatcgattc ggagaacatt cgcaacatca
    14221 gatcgaaata acgcgcgaag gccacgccca ccagagcaaa cgggaagtac ccccgcccgt
    14281 atttgcccag tcccgcagat ccgctcgcgg gaatcaactg cccgacgaag tgcaggatga
    14341 ccagcagcat gatcgactcc agcaacccca tcagaaacgc cgcctgatag ccggattcga
    14401 tccggaaatc tcgccggatg aacgcgcccg ctttgctgag aaaggtgctc atgccgcact
    14461 tccccccgca acgcaccggc ggtagtagtc cagcagcgtg gttccctctt ctttcagatg
    14521 ggccaggttc acgaacccgc gcattcggcc cccgtcaatc accatcactt tgctgcataa
    14581 cgtcaccgcc tcttccggcc ggtgcgtcgc aatcaggagc gtcttgcgcg agccgcgatg
    14641 gatatccgtc aggaccaggt tgaccatttc atcggcggcc accggatcga gcgcccgggt
    14701 cggctcgtcg aggatcaaca cttcgggatc ggaaagcagc acgcgtgcca gagccagccg
    14761 ctgcttcatc cccgaactgt agccggacac caactgatcc ccggctcttt ccagacccac
    14821 aagctgcatc agttcccgga tgcgctcctt cagagcttgc ccgcggagat tgtccagggc
    14881 accgaagaat tccaggttct gcactccagt cagccgccag tagaagcttc gctcttcgtt
    14941 gaagacgaac cctaccgact tgcgcgcggc ggcgttgtca tggagcgtac ttaggccgtt
    15001 tacggtaact tcaccttcag tcgggaacag gagcccgctg atcaacttca gcagtgttgt
    15061 cttgcctgcg ccgtttggcc ccatcaccgc gatacggtcg cctgcctgaa tatccaccga
    15121 tgcattttgc aacgcggtga ccactcggcg cgcccgcagc ggatgagtca cccattccct
    15181 gtatcgcttc gggatgggaa agcgcatcgt cacgtcgcgc accgcaatgg ccggcgctga
    15241 agacggttcc tgaacagggg actccacgac cgccggtttc tccatgacga tagatttcag
    15301 gctactcaac gcaacgcctc cgccggctgt tccgcctggt cggcttcggt ggctttctta
    15361 atgcagcgga gaatcctttc agcggcggct ttgcgatgat cggtgaacat cgtttcgtga
    15421 ctgcccggac tggtcagaat ctcgagaccg ccatcggcaa atcccgtcca tcccaggtcc
    15481 gaattgaacc ggtgctgctc ctcattcaca ataagcacca gccttccggc atatttttgc
    15541 ggacaatgcg catacagcat ccgccagtac cgcaccttcg agttgtaata ccggtcctgc
    15601 tccaggatct caggcctgtc ctcgatgccc atcttccgtc ggaacagcgt gcgcaccatc
    15661 tgcacctttt gccgtttatc cgcgtgcgcg atctccgcga gcactccgcg aatatggcgg
    15721 atgcgccgga gaatatagtt ccgttccgcc gcccgtgccg tgtcggggcc gggccgatcg
    15781 gcatccacca gcagcatgag ttgaatctgc tcgccctgtt ccatcagggt ccgggccagt
    15841 tcgagagccg cgatgccgcc gacgcacatt ccgcccagca gataaggccc atggggctgg
    15901 accgcccgca gctctttgag gaactcgcgc gccatctctc cgaccgactc gtaatcggcg
    15961 cctccaaagg tccatcgcgg ccggaatcca aagaccggct ggtcctttcc gaagtactga
    16021 atgagtggtg tcaggaactg cagcgtatcg tcgggattct ggtaaccggt aataaaaaag
    16081 aaaggcgtcc gcgaacctct gggctgaaca gggaccaggc aattccactc cggtatctcg
    16141 gaactgtatc ggtatccgcc ctgcgtcgcg tcataggcga tatggcttgc caggcccgca
    16201 atcgtcggat cgagaaagac actacgcaga ggaatctcca tctcgagttc agtgcgcagc
    16261 cgcgccacga cagatgcggc cgacaacgag tgtccaccga gcacaaagaa gtcgtcgaag
    16321 atcccaaatt gatcgaggtg taacacctcg cgccatacct cagccatcac cgcttcaacg
    16381 tgatttctgg gctgttccgc agagaggcca tgaacggtgc cttttgctgc gccgatgcca
    16441 tcgcttcccg ccactttggg ttccggcgca aattccggac ccatcagtgc tttgcggtca
    16501 actttccggt ttgccgtcag cgggaatgca cccagctcca aaaataacga tggaatcatg
    16561 tactcgggca gactcttctt gagtgtcgcc cgcaacgctg cggcatccgg aaccgatccg
    16621 ttttgcgttg tcagataggc gaccagacga ggatcactcg ctacgtcctc ccggaccacc
    16681 gcaaccgcct gctgaatttc ggaacgctgc tggagggctg cttcaatttc gcccagctcg
    16741 atgcgatacc cgcgcagctt cacctgatgg tctgcgcggc ccatgcattc gaaacgcccg
    16801 tcggacaatc gccgtgccag atcgccggtc cgatacagac gcgcgcgata gcgttcgctc
    16861 tgaataaatc gcgcgcgatt ctcttcttcg cgattgcggt acccacgcgc cagcccctgt
    16921 cctccgatat gcaattcgcc aacaacgccg ggcggcaccg gagcaccgtg ctcgtccagg
    16981 acgtaaatat ccgtgttggc gattggccgg ccaatggtaa ccttgctgtc gttctgcacc
    17041 cggtccaccg tcgaccagat cgtggtctct gtaggaccgt agaggttcca tacctc
//
LOCUS       DASZYN010000399         2334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_531044, whole genome shotgun sequence.
ACCESSION   DASZYN010000399 DASZYN010000000
VERSION     DASZYN010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531044"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VHE33_10250"
     CDS             complement(<1..950)
                     /locus_tag="VHE33_10250"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HVT97880.1"
                     /translation="MKAGFAGFAAGSMICWGMLIGGQTALGQFAGPAVGGPSTVSSVP
                     PSAMNIQLQDVKIVPGDVISIATYGAPELSTGGSTSVDSPTGNGSIGASGIKVGTQGE
                     IILPYLGVVKVGGRTPSEVALYLERELKEKGILVDPQVSVSLVASPTRVITVVGEVQK
                     PAPVPAFGTLRLLDVISACGGFTPLASHTITVRRPGEAQPITVIMGTDPRTTDETNIP
                     LMAGDTVIVPKVGNVFVIGQVKSPSAIPLSSNAPITVMRAIAMSGGVNFGAALSRVVV
                     IRKTPNDQHVEIQMDLKKIMQGKQRDIALASDDVLLVPGNG"
     gene            complement(993..>2334)
                     /locus_tag="VHE33_10255"
     CDS             complement(993..>2334)
                     /locus_tag="VHE33_10255"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HVT97881.1"
                     /translation="GLPSIAGVSGAGGSEGLLANLRTQRAGIEAELAHLQPVYGPNYP
                     QVKQLRAQDESLVKQIATQEHRVVEQANDSLNMAQAAESKAQALLNDKIHGVYGQRDD
                     LVQYMLLNQEYDTNRKMYESIVGRLREAAIDAGLDAADISIVDFASTPLNPSSSSPVK
                     LGLIGVLFGLFGGFTLALLLEKMDTRMRDAHEIQEMLGLPSLAMIPQSHWKSKGSELE
                     WVVGPELLRDPRSPFSESFRALRTSMRLSTTSRESKAIAVTSCQPSEGKSTVSVNMAA
                     VLAQGGKKVALVDTDMRRPSVYKRLHLEGGKGLSEVLTGYYTLDEVTQTHEILTTLDV
                     IPSGTVPPLPADLLASDQMSEVVRQLRERYDYVIFDTPPLLSVTDPAIVAAQADGMVL
                     VIRQGYCTRRMLARASEILHELDVKVYGFVFNGVDASLPEYYGYLGYYTYEYGK"
BASE COUNT          484 a          747 c          614 g          489 t
ORIGIN      
        1 ccattgcctg ggaccagaag cacatcgtcc gatgccagcg cgatgtcccg ctgcttgccc
       61 tgcatgatct tcttcagatc catctggatt tccacgtgct ggtcgtttgg agtcttacgg
      121 ataacaacca ccctggacaa agccgccccg aaattcactc cgcctgacat cgcaatcgcg
      181 cgcatcaccg taatgggcgc gttgctcgaa agcgggatag ccgatggtga cttgacctga
      241 ccgatgacaa acacattgcc cactttaggc acaatcaccg tatcgccggc cattaacggt
      301 atgttggtct cgtccgtggt cctcggatcc gttcccataa tcaccgtaat cggctgtgcc
      361 tcgccaggcc gccgcaccgt gatcgtgtgg ctcgccagcg gcgtaaatcc gccgcaggcg
      421 gaaataacat cgagtaaacg cagagtgccg aatgccggaa caggcgcggg tttctgaacc
      481 tctcccacaa ccgtgataac ccgggtcggt gaagccacca gcgagacact cacctgggga
      541 tccacaagaa tccctttctc cttcagttcc ctttcgagat agagtgcgac ttcggaaggg
      601 gtccggcccc ctaccttcac gacccccaga taaggcagga taatctctcc ctgcgttcct
      661 actttgatgc cgctggcacc aatactccca ttccccgttg gcgaatccac gctggtagaa
      721 cctcccgtgg acagttccgg tgcgccgtag gtcgcaatcg aaatcacatc tccgggtacg
      781 atcttcacat cctggagttg aatattcatc gcggaaggcg gcactgagga aaccgtcgac
      841 ggaccgccca ccgcgggtcc ggcaaattgc ccgagtgccg tctggccacc gatgagcatc
      901 ccccagcaga tcatgctgcc cgctgcgaat cctgcaaaac ctgccttcat ttgaataact
      961 ctcacccagc tctcgcggtt aatacagagt gcttatttcc cgtattcgta ggtgtaatac
     1021 ccaagatatc cgtagtattc cggtaacgaa gcatcgacac cgttaaacac aaagccgtag
     1081 accttcacgt ccagttcgtg gagaatttca gaggctcgcg ccaacatcct gcgcgtgcaa
     1141 tagccctgcc ggatgaccaa caccattccg tcggcttgcg cggcgacaat ggctggatcg
     1201 gttaccgaaa gaagaggcgg ggtatcgaag atgacgtagt cgtaccgctc ccgcagctgg
     1261 cgcactactt cgctcatctg atccgatgcc agcaggtccg caggcagcgg aggcactgtg
     1321 cctgagggga tcacgtccag cgtcgtcaaa atctcgtgcg tctgagtgac ctcatcgagg
     1381 gtgtaatagc cggtgagaac ttccgacagt cccttgccac cttcgaggtg aagccgtttg
     1441 tagaccgaag gacgccgcat atctgtgtcg acgagggcta ccttcttgcc accctgcgcg
     1501 agcaccgccg ccatgttcac tgaaacggtg gacttacctt cactgggctg gcagctggtg
     1561 accgcaatgg ccttcgactc tctggacgtg gtcgacaacc gcatcgaagt acgaagagcc
     1621 cggaacgatt cggagaatgg cgatcgcggg tcgcgcagca gctccggacc caccacccac
     1681 tccagttccg agcctttgct cttccagtga ctctgcggaa tcatcgccag cgacggcaat
     1741 cccagcatct cctggatttc atgcgcatcc cgcatccggg tatccatctt ctccagcagg
     1801 agcgccaagg tgaagcctcc gaagagcccg aacagcaccc cgatgaggcc cagcttcacc
     1861 ggagacgagc tggagggatt cagcggcgta gaggcgaagt cgacaatact gatatcggca
     1921 gcatccagtc ccgcgtcgat ggcagcttcg cgcagccgcc ccacaatgct ttcgtacatc
     1981 ttgcggttgg tatcatattc ctggttcagc agcatgtact gcaccagatc gtcacgctgc
     2041 ccgtacacac cgtgaatttt gtcgttcagc agggcctgcg ctttcgactc ggcggcctga
     2101 gccatgttca gtgagtcgtt ggcctgctcc actacgcggt gctcctgcgt ggcgatctgc
     2161 tttaccagtg actcgtcctg ggctctcaac tgttttacct gcggataatt cggtccataa
     2221 accggctgca ggtgcgcgag ttcggcctcg atgcccgccc gctgcgtacg aagattcgcc
     2281 agcaaaccct ccgagcctcc tgctccgctt actcctgcaa tgctcggaag tccc
//
LOCUS       DASZYN010000400         1780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_725511, whole genome shotgun sequence.
ACCESSION   DASZYN010000400 DASZYN010000000
VERSION     DASZYN010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725511"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VHE33_10260"
     CDS             <1..232
                     /locus_tag="VHE33_10260"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT97882.1"
                     /translation="KAFSETDFNDDLKKIDVPTLVMHGDDDQIVPFHDAGELTAKIVK
                     GAQLKVYKGYPHGMAQIHAEEINNDLLAFIES"
     gene            945..1112
                     /locus_tag="VHE33_10265"
     CDS             945..1112
                     /locus_tag="VHE33_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97883.1"
                     /translation="MTSIKVFVRNLEPDRARPPISIQRPRLVPAPDSPDHEEEPTLAE
                     RVFHFLDRRSA"
     gene            complement(1125..>1780)
                     /locus_tag="VHE33_10270"
     CDS             complement(1125..>1780)
                     /locus_tag="VHE33_10270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT97884.1"
                     /translation="TDHAGLALGGPEARRYPVEIGPLSGMPVETETHWAALAELVEPE
                     GVVGLFFRETPCIPEDWTVVRSGELVQMVAEDPKPVAIEEPEGVTLRVLTKEDAGAMV
                     ELATLTEPGPFRLRTHELGTFFGIFADGRLVAMAGKRMHVPGYVEVSAVCTHPEARGR
                     GYARLLMSCVMEEIVAAGKTPFLHSLASNAGAIRVYEDLGFRVRRGLAFAAVARSRE"
BASE COUNT          359 a          672 c          428 g          321 t
ORIGIN      
        1 caaggccttc tctgaaaccg acttcaacga cgatctgaag aagatcgacg tccctaccct
       61 cgtcatgcac ggcgatgacg atcagatcgt cccctttcac gatgctggcg aactcacggc
      121 aaagatcgtc aaaggcgccc aactcaaggt ctacaaaggc tacccgcacg gcatggccca
      181 aatccacgct gaagagatca acaacgacct cctcgccttc atcgaaagct agtgtggtgt
      241 ttctgaagtt tcctttacct tgtcatcccg accggaccct gagcgtagcg aagcggaagc
      301 ggagggacct gcttctcttc cgcataaaga aatttcggaa acaccacacg agtggcctgt
      361 cgcagaggtt tcgttcgtgg ctttgaaagg gctcggcttc agccgtccag agccaaactg
      421 aaacgggctt cagcccccgg cgaaagatct tttcatcgcc attcccgcta acgttttctc
      481 tgcgacaagc cacgagggct tcgaccggat gatcgggtcc ttcccgcgaa gcggtgccgc
      541 gccagcggca cgacggtaga agccccggcc ttcaggccgg ggaaaaagga tccgcgggtt
      601 cgaagccttt aggcccggaa cgtactcaat cacgcggtca tggtcctagc cagacgcagg
      661 aagcaggaag cgccgccaca gtgtccggtg ttccagcttc ctgcatcacc tgccgcccag
      721 ttaaccgtcc cgccaacaca cccgtcacgc cagtacccca gcaaaaactc agtagtaacg
      781 cggccttgca cgcgcttccg gaaaatgcca ctatcgattc gtacgcgctg ccggcgacct
      841 atccgcacta ccctgcggca catcctcgaa agaagagttc gtttgtaatc gccctcggct
      901 gtctccgatc ggctttgttt caatttctgc tctggagttg tttcatgaca tcgatcaaag
      961 ttttcgtgcg caaccttgag ccggaccgtg cgcgcccgcc tatctccatc cagaggcccc
     1021 gccttgtccc ggcgccagac tcccccgacc acgaagaaga gccaacgctg gccgaacgcg
     1081 tcttccactt cctcgaccgg cgcagcgcat aaacgactgc gcccctactc cctgctcctc
     1141 gccaccgccg caaacgccag cccccttcgc acccgaaacc cgagatcttc atacacccgg
     1201 atcgctcccg cattgctcgc cagcgagtgc agaaacggcg tcttccccgc cgccacaatc
     1261 tcctccatca cgcacgacat cagcaaccgc gcatatcccc gcccccgcgc ctcgggatgc
     1321 gtgcacactg cgctcacctc cacatatccc ggcacgtgca ttcgtttgcc cgccatcgcc
     1381 accagccgcc cgtccgcgaa aatcccgaag aacgtgccca gctcatgggt gcgcagccgg
     1441 aacggccccg gctcggtcag cgtcgccagc tccaccatcg ccccagcgtc ttccttcgtg
     1501 agcacacgca gcgtcacccc ctccggctcc tcaatcgcca ccggcttcgg atcttccgcc
     1561 accatctgca ccagctcgcc gctgcgcacc actgtccagt cctcaggaat gcacggcgtc
     1621 tcgcgaaaga acagcccgac cactccctcc ggctccacca attccgccag cgctgcccaa
     1681 tgcgtctccg tctcaaccgg catcccgctc agcggaccga tctctaccgg ataccgccgc
     1741 gcttccggcc cgcccagcgc cagccccgca tgatccgtcg
//
LOCUS       DASZYN010000401         5340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_336698, whole genome shotgun sequence.
ACCESSION   DASZYN010000401 DASZYN010000000
VERSION     DASZYN010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336698"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VHE33_10275"
     CDS             <1..286
                     /locus_tag="VHE33_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97885.1"
                     /translation="SAPEEKECPECKGPIERLLSAPAIQFKGSGWYVNDYAGKSKSGG
                     SMKASADGEAKAGDSKSEKKPAEKSSSASSDSGSSSTSAAPPSSSASKSD"
     gene            357..893
                     /locus_tag="VHE33_10280"
     CDS             357..893
                     /locus_tag="VHE33_10280"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HVT97886.1"
                     /translation="MAATLPSEKAASERISPSAVAPEALHPLYIAGNYLRTATTLESP
                     LRNLVYLRASQMNGCAFCIAMHWREAKEDGLSDDHLHGLLAWREAPWYTDRERAALAW
                     TEALTDIAHSHAPDDVYDQARRQFTDRELVDLTLAITTINAWNRFMAAFRVNPETAPD
                     VIAVLRNTPEANSSAAAR"
     gene            complement(992..2956)
                     /locus_tag="VHE33_10285"
     CDS             complement(992..2956)
                     /locus_tag="VHE33_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HVT97887.1"
                     /translation="MLQLSGAGKRFGPRLLFENADWLITPNEKTALVGANGAGKSTLM
                     KVLGGMESLDYGSMQRMKGMTIGYLPQDGLTLSGRTVFEECLSVFDELRAMEQEFEQL
                     AHALSELDPASKEYEAAADRYSHVEGRLRHHDVYALDAQVGGVLTGLGFSKEDWTRRT
                     EEFSGGWQMRIALGKLLLEKPDLLLLDEPTNHLDLETRNWLENYLSTWENGYILISHD
                     RYFLDVTVNKIIEIWNKALHVYHGNYEKYLTQKAERRAQLEAAYKNQRDRIEQLEVFI
                     NRFRYQATKAKQVQSRIKELDKIERIEIPLEEPVPHFTFPQPPPSGRNVVDVEGLSMQ
                     YGEKRVLENVNFTIERGDRIALVGVNGAGKSTLIRLLSGQDTPTQGTLKLGHNVIAEY
                     FAQDQYKVLDHSARMLDDMGRAAPKVAEKDLRSLLGCFLFSGDDVFKPLGVLSGGERN
                     RYALARILVSPANFLLLDEPTNHLDLRAKDVLLEAIGKFNGTVIFVSHDRYFIDGLAQ
                     KVFEIEKNPDDPARGSRLNVYPGNYEDYRWRKEGGAAELAAATVAAAEPIVKTPVVVV
                     VPEPVVEEKAAKRVNPIKLRQMQERLAAVEAQIPKVETSLAETEQALGVYVSAEETQR
                     LSDALTALREQQASLNSEWEELSMQLEEAR"
     gene            3172..>5340
                     /locus_tag="VHE33_10290"
     CDS             3172..>5340
                     /locus_tag="VHE33_10290"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HVT97888.1"
                     /translation="MKKSSAVATSARKSAPPAYKNPALPSARRVKDLLSRMTLDEKAA
                     QMLCVWQEKAQKLVDEKGDFDLAKAKKAFRDRRGLGQVGRPSDAGGGKKARAMAELTN
                     AIQKFFLENSRLGIPVMFHEECLHGHAAPDGTSFPQPIALGATFNPDLVEQLFAMTAL
                     EARLRGAHQALTPVVDVAREPRWGRVEETYGEDPYLVSRLGIAAVRGFQGDRNFRDKK
                     HILACLKHFVGHGQPESGMNCAPANVSERVLRETFLYTFKEALREAEAVTIMASYNEV
                     DGVPSHANKWLLKDVLRREMGFKGFIVSDYYAIWELGYRPDTHGHFVAKDKKESCALA
                     VNAGVNIELPDPDCYLHLVELVKKGVLKESQLDELVAPMLFWKFEMGLFDDPYVDPTE
                     ADRVVGSDAHKKLARTAAQEAITLLKNDNNTAPLDINKIKTIAVIGPNANRTLLGGYS
                     GVPKHEVTVLEGIRNRVGNRAKVLYSEGCRITIGGNWNQDAVVPSDPVEDRKQIQEAV
                     EVAKQADVIVLAIGGNEQTSREAWNLQHMGDRTSLDLIGRQDELVEALAALGKPIVAF
                     LFNGRPLSINHLLAHVPVIFECFYLGQETGHAIADVLFGEFNPSGKLPISFPRSVGHL
                     PVFYNYKPSARRGYLFDDVSPLFAFGYGLSYTTFELKNPRLTKKKITAKGATQVQVDV
                     TNTGKRAGAEVIQLYIRDLVSSVTRPIKELKGFRKIYLKPG"
BASE COUNT         1012 a         1861 c         1476 g          991 t
ORIGIN      
        1 cagcgctccg gaagaaaaag agtgccccga gtgcaagggc cccatcgaac gcctcctttc
       61 cgcgcccgcc atccagttca aaggctccgg ctggtacgtc aacgactacg ccggcaagtc
      121 gaagtccggc ggctccatga aagcgtcggc cgacggcgaa gccaaagcag gcgactcgaa
      181 atccgaaaag aagcccgccg agaagtcctc cagcgcatcg tcggattcgg gcagctcgag
      241 cacttcagcc gcgccccctt cctcgagcgc gtcgaagagc gactaacgcc gcatccggca
      301 cgctgcaacg ctcatcgaag agagaagatc caccccgagg tggctcttct ttccccatgg
      361 ctgcaacact cccctctgaa aaagctgcgt ccgaacgcat ctctccctcc gcggtagcgc
      421 ctgaagcgtt gcatccgctc tacatcgccg gcaactatct gcgcaccgcc accacactcg
      481 aatcgccgct gcgcaatctg gtttatctcc gcgcctcgca gatgaacggc tgcgctttct
      541 gtatcgcgat gcactggcgc gaggccaagg aggacggcct gagcgacgat catctccatg
      601 ggctgctcgc ctggcgcgaa gcgccctggt acacggatcg cgagcgcgcc gcgctggctt
      661 ggaccgaggc cctcaccgac atcgcccaca gccatgcccc ggacgacgtc tacgatcagg
      721 cacgccggca attcaccgat cgcgaactcg tcgacctcac cctggccatc accacgatca
      781 atgcgtggaa ccgctttatg gccgccttcc gcgtgaatcc tgagaccgcg cccgacgtga
      841 tcgccgtgct gagaaacacg cccgaagcaa attcgtctgc ggcggcacgg tgaataagct
      901 cggcagcaac attcggctcg cggttagcgg ctcgcctgtc gctaaccgct tcatcagcgg
      961 accgcttctt agctagccgg acttagctgg ctcaccgggc ttcctccaac tgcatcgaca
     1021 gctcttccca ctcgctgttc agcgacgcct gctgttcgcg caatgctgtg agcgcatccg
     1081 aaagccgctg cgtctcttcc gcgctcacat acacccctaa cgcctgctcg gtctcagcca
     1141 gcgaagtctc gactttggga atctgcgcct ccaccgcggc cagccgctcc tgcatctgcc
     1201 gcagcttgat cggattcacc ctcttcgctg ccttctcctc aaccacaggc tccggcacga
     1261 ccaccacgac gggcgttttc acgatgggct ccgctgcagc cacggtcgcg gctgccagct
     1321 cggctgcccc gccctccttg cgccaccgat agtcctcgta gttgcccgga taaacgttca
     1381 ggcgcgaacc ccgcgctgga tcgtccgggt tcttctcaat ctcaaagacc ttttgcgcca
     1441 gcccgtcgat gaagtagcga tcgtgcgaga cgaagatcac cgtgccgttg aacttcccga
     1501 tcgcctccag cagcacgtcc ttcgcgcgca ggtcgagatg gttcgtcggc tcgtccagca
     1561 gcaggaagtt cgccgggctg accaggatgc gcgccagcgc atagcggttg cgctccccgc
     1621 cgctcagcac gcccagcggc ttaaagacgt cgtcgcccga aaacagaaaa caccccagca
     1681 ggctgcgcag gtctttctcc gcaactttgg gcgccgcgcg gcccatgtca tccagcatcc
     1741 gcgcgctgtg atcgagcacc ttgtactgat cctgcgcgaa gtactctgcg atgacgttgt
     1801 ggcccagctt cagcgtgccc tgcgtcggtg tgtcctgccc gctcagcagc cggatcagcg
     1861 tcgacttccc ggcaccgttc acgcccacca gcgcgatccg atcgccgcgt tcgatggtga
     1921 agttcacgtt ctccagcacg cgcttctcgc cgtactgcat ggacagccct tcaacatcga
     1981 cgacgtttct ccctgaaggc ggcggctgcg gaaacgtgaa gtgcggcacc ggctcttcca
     2041 gcggaatctc gatgcgctcg atcttgtcga gctccttgat gcggctctgc acctgcttcg
     2101 ccttcgtcgc ctggtagcgg aaccggttga tgaacacttc cagctgctcg atgcggtcgc
     2161 gctggttctt gtaggccgcc tccagctgcg cccgccgctc cgccttctgc gtcaggtact
     2221 tctcgtagtt gccgtggtag acgtgcagtg ccttgttcca gatctcgatg atcttgttca
     2281 ccgtcacgtc gagaaaatac cggtcgtgcg agatcaggat gtacccgttc tcccaggtgc
     2341 tcaggtagtt ctcgagccag ttgcgcgttt cgagatcgag atggttcgtg ggctcgtcca
     2401 gcagaagcag gtcgggtttc tcaagcagca gctttcccag ggcgatacgc atctgccagc
     2461 cgccggaaaa ttcctccgtc cgccgcgtcc agtcttcctt gctgaagccg agccctgtga
     2521 ggactccgcc cacctgggcg tcgagcgcat agacgtcatg gtgccgcagc cgcccctcga
     2581 catggctgta gcgatcggcc gctgcctcat actccttgct cgccggatcc agctccgaca
     2641 gcgcgtgcgc caactgctcg aattcctgct ccatcgcccg cagctcgtca aagacgctca
     2701 ggcactcctc gaagacggtc cgcccgctca gggtcagccc atcctggggc aggtagccga
     2761 tggtcatgcc tttcatccgc tgcatcgagc cgtaatcgag cgactccatg ccgcccagga
     2821 ccttcatcag cgtggatttc ccggccccgt tcgcccccac gagcgcggtt ttctcattgg
     2881 gggtgatcag ccagtcagca ttttcaaaga ggagtcgcgg accgaagcgc ttgcccgcgc
     2941 cggagagctg cagcatagcc ctattttaac gggccggcgg ctggccccct ggctcgcctg
     3001 gcgtgagggt cgatcatacg ccacgaagta actgtcgggg cgtccttcag tcacctgcgt
     3061 aacggcgatt tttgattgct ctttgaagaa caggcgacta catttagttc ggcatgctaa
     3121 caattgcgca cgcatgccgt actccgtcgt gatcaggaat caggattctc catgaagaag
     3181 tcatctgccg tcgcaacttc cgcgcgcaaa tccgcgccgc ctgcctacaa aaatcctgcg
     3241 ctgccctctg cgcgccgcgt gaaggatctg ctctcccgca tgaccctcga cgagaaagct
     3301 gcgcagatgc tctgcgtctg gcaggagaaa gcgcaaaagc tcgtcgatga aaaaggcgac
     3361 ttcgacctcg ccaaagcaaa gaaagccttt cgcgaccgcc gcgggctcgg ccaggtcggc
     3421 cgcccctccg acgccggcgg cggcaaaaag gcccgcgcca tggcagagct gaccaacgcc
     3481 attcaaaagt tcttcctcga gaactcccgc ctcggtattc ccgtcatgtt ccacgaggag
     3541 tgcctccacg gccacgctgc gcccgacggc accagctttc cccaaccgat tgccctgggc
     3601 gcgaccttca acccggacct tgtcgagcag ctcttcgcta tgacggcgct ggaagctcgc
     3661 ctgcgcggcg ctcaccaggc cctcacgccg gttgtcgacg ttgcccgtga gccccgctgg
     3721 ggacgcgtgg aagagaccta cggcgaagac ccgtacctcg tctctcgcct cggcatcgcc
     3781 gcggtgcgcg gcttccaggg cgatcgtaat ttccgcgaca agaagcacat tctcgcgtgc
     3841 ctgaagcact ttgtcggcca cggccagccc gaatccggca tgaactgcgc cccggccaac
     3901 gtctccgagc gcgtcctgcg cgagaccttc ctctacacct tcaaagaggc actgcgcgaa
     3961 gccgaggccg ttacgatcat ggcctcctat aacgaagtcg acggcgtccc ttcacacgcc
     4021 aacaagtggc tcctcaaaga cgttctgcgc agggagatgg gctttaaggg cttcatcgtc
     4081 tcggactact acgcgatctg ggaactcggc taccgccccg acacgcatgg ccacttcgtc
     4141 gcaaaggaca aaaaagaatc ctgcgcgctc gccgtcaacg ccggcgtcaa cattgagctg
     4201 cccgacccgg attgctacct gcatctcgtc gagctggtga agaagggcgt gctcaaagaa
     4261 tcgcagctcg atgagctggt cgcgccgatg ctcttctgga aattcgaaat gggcctcttc
     4321 gacgatccat acgtcgatcc caccgaggcc gaccgcgtgg tcggctccga tgcccacaaa
     4381 aagctcgccc gtaccgccgc ccaggaagcc attacgctcc tgaagaacga caacaacacc
     4441 gccccactcg acatcaacaa gatcaagacc atcgcggtca tcggcccgaa tgccaatcgc
     4501 acgctgctcg gtggatactc cggcgtgccg aagcatgaag tcacggtgct cgaaggcatt
     4561 cgcaaccggg tcggcaaccg cgcgaaggtc ctgtacagcg aaggctgcag gatcaccatc
     4621 ggcggcaact ggaaccagga cgccgttgtg cccagtgacc ccgtcgaaga ccgcaagcag
     4681 atccaggaag ccgtcgaagt ggcgaagcag gccgacgtca ttgtcctcgc gattggtggc
     4741 aacgaacaga cctcgcgcga agcctggaac ctgcagcaca tgggcgaccg caccagcctt
     4801 gacctgatcg gccgtcagga cgagctggtc gaagccctgg ccgcgctggg caagcccatc
     4861 gttgcgttcc tgttcaacgg ccgcccgctt tccatcaacc acctgctcgc gcacgtgccg
     4921 gtgatcttcg agtgcttcta cctcggccag gaaacgggac acgccatcgc cgacgtgctc
     4981 ttcggtgaat tcaatcccag cggtaagctg ccgatcagct tcccgcgttc ggtcgggcac
     5041 ctgcccgtct tctacaacta caagccttcg gcgcgccgcg ggtacctgtt cgacgacgtc
     5101 tctccgctct tcgccttcgg ctatggcctc agctacacca ccttcgaact gaagaatccg
     5161 cgcctcacca aaaagaagat cacggccaaa ggcgcgaccc aggttcaggt ggacgtcacc
     5221 aacacgggca aacgcgcggg cgccgaggtg attcagctct acatccgaga ccttgtcagt
     5281 tcggtcacgc gcccgatcaa agagctcaag ggcttcagga agatctatct caagcccggc
//
LOCUS       DASZYN010000402         6780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_918171, whole genome shotgun sequence.
ACCESSION   DASZYN010000402 DASZYN010000000
VERSION     DASZYN010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918171"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            724..1647
                     /locus_tag="VHE33_10295"
     CDS             724..1647
                     /locus_tag="VHE33_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97889.1"
                     /translation="MVLVPGRRCVVSFMAALALALPGLHGQVSYTSMPLDQGFGPLDP
                     TPPAIPVQQIITEFTAKEAVFRHALDNYTWTRSVRVQTIDDDGKPDGQYYQVVDIAFD
                     ADGHRRERVLDAPESSLTRVMMSPADFEDIEERLPFVLTTEDAPQYDITYVGKQKIDE
                     IDTWSFDVKPKVIEKKKRYFQGRIWVDQKDHQIVVTNGKNVPDDTRPGHEDLSTPFVT
                     YRQLVDGKYWFPVYTHGDGVLHFTAGHGYLSDNVHLRETVKYTNYHEFHTSIRVLYNG
                     QDITGSPEKGTAGQGQAPAPAPKPEDPKKPQ"
     gene            complement(1699..2298)
                     /locus_tag="VHE33_10300"
     CDS             complement(1699..2298)
                     /locus_tag="VHE33_10300"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HVT97890.1"
                     /translation="MGKVIAIVGSYRKGGITDQAVDAVLEGARVAGAQTAKIYLIDQN
                     IQFCRNCRTCAAQNGLTPGRCVQQDDMESVLRAVDDANAIVLGSPVNYYNVTAVFRKF
                     MERLLGFAAYWPWGRNHGPKLRSRILHKKAVLVCSAGMPGAMIPFASGATSSLKLTAR
                     LLGAAPSARLWIGRSAVSPDQQIDSRTRDRAVSLGRNLA"
     gene            2572..3756
                     /gene="metK"
                     /locus_tag="VHE33_10305"
     CDS             2572..3756
                     /gene="metK"
                     /locus_tag="VHE33_10305"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HVT97891.1"
                     /translation="MPAAERFLFTSESVTEGHPDKIADQVSDAILDACLEQDPYSRVA
                     CETLTATGLVVIAGEITTRAYVDFQALVRGVVTAIGYDNAVYGFDSNTCAVISTINKQ
                     SGDIAMGVDTGGAGDQGMMFGYATNETPELMPTPISLAHKLTRRLSEVRKNGKLPYLR
                     PDGKSQVTVEYDKNHKPVRIDAVVISTQHAETVSTEELRADILKHVIQSTLPAELLDE
                     NTKYHINPTGRFVIGGPMGDTGLTGRKIIVDTYGGMGRHGGGAFSGKDPTKVDRSAAY
                     MARYIAKNIVAAGLAERCEVQLAYAIGVAEPVSVLVDTFGTSKLDETALVALVRKNFE
                     LTPKGIMESLNLRRPIYRKTAAYGHFGRVNEPDFTWETTDKAAALKDGAKAHAKEPVS
                     AK"
     gene            complement(3809..5842)
                     /locus_tag="VHE33_10310"
     CDS             complement(3809..5842)
                     /locus_tag="VHE33_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT97892.1"
                     /translation="MTAHKNCWILAASALLLAGATAPSAIAAKKPGAQDLFSMTLEQL
                     TNVQVSSVSRHDEPLFDTPAAVFVITRDDIHRSGATSIPEVLRMVPGVEVAQIDANKW
                     AVSARGFNNRFANKMLVMIDNRTVYNQLYSGVFWDQTDVLLEDVERIEVVRGPGATLW
                     GANAVNGVINIVTRKAQDTQGALVVAEGGRIDQEAVARYGGHLGASADYRLYGKYLHR
                     DALDAADGASAGDTANIERAGIRLDWSPRLRDSITLQADLYDNAEQQRVNFDYAATTS
                     TLAPIHAAGGFASVRWEHTRGASSSTILQAYLRDDRRFEIEQSVHMHSFDLDLQDRHS
                     LGTRQNLVWGAGYRWTSDVAAAAEPMFQHPDHVIHLSSGFLQDEIALLPRRLTLTAGA
                     KLLWNTYSHFEYQPGARLLAKLSPAQTLWASVSRAVRTPSDIDRDDRLDFAGGVIQGL
                     PVTIQISGNPRFGSEIVHAYEAGYRHQFGNSVSVDLAGFVNHYYSLGATAIGAAVLHF
                     NPAPSIVLPCTYLNALSSDSQGAEVSLLWNPDTTLHFQGSYAWMQNRFSDHGTPTDVP
                     LNNQDWAAPRNAFDVRGFWSPAPQWSLGAILNGNSPTASSMGGALNNPSAFIVPAHTR
                     LDLRLSRTLGEKLEISAGATNLLQARHLEFPSIDYVAASRIPRSAYLRLQWTH"
     gene            6015..6506
                     /locus_tag="VHE33_10315"
     CDS             6015..6506
                     /locus_tag="VHE33_10315"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HVT97893.1"
                     /translation="MRRFVIFAVICGVALLCRGQETAIRADRIVIVKSAHRMTLMSRG
                     REIRSYKVALGDPIGPKVQRGDKKTPEGVYFVDGKNANSLFHRALHLSYPNAADRARA
                     RKMGVNPGGDIEIHGLPRQYAWMGAAHRSIDWTTGCIAVTNAEIDEIWEEVRVGTAVE
                     IRP"
BASE COUNT         1353 a         1727 c         2459 g         1241 t
ORIGIN      
        1 ggtaccttcg tgcggacgca gcttcgaatt gggttgaggt tctgctcgga aaggcttccg
       61 ctacttgctg tggggtgaac ctggcctatt ttcgggtggc tcccccaaat cagaattcgg
      121 accctattga gagtccgggt caaaattgag agtccgggtc agatgaagag tcctggtcaa
      181 attgagagtc cgggtcaaat caagagtccg ggtcaaattg agagtcctgg gtcaaatgaa
      241 gagtccgggt caaatgaaga gtcctgggtc aaatgaagag ttcgggtcaa atgaagagtt
      301 cgggtcaaat gaagagtccg ggtcaaaatg aagagtccgg gtcaaatgaa gagtcctggg
      361 tcaaatgaag agtcctgggt cgaatgaaga gtccgggtca aatgaagagt ccgggtcaaa
      421 atgaagagtc cgggtcaaac agcggcgagt gcgggaacag gtggcatgga gagatggcac
      481 agatcgcagc ggcacagatc gtcggggcac agcttacagg ggcattggca ggaacaagtc
      541 gccgcagcat cggccgcagg agtccattat caggggacag ggtgcagggg acagggtgcc
      601 caactcaagt tgcggtgtgc agaagtgggc acagccacag ctgacgttgc cgccaagggc
      661 gagccggatg cgtctattct ttagtagtca gtagccgaag cagaagccag caggagtcat
      721 gtcgtggttt tggtccccgg acgtcgttgc gttgtgagtt tcatggccgc cctggcgctg
      781 gcattgccgg gcctgcacgg gcaggtttcg tacacctcga tgccgctcga tcagggtttt
      841 ggaccactgg atccgacgcc gcccgcgatt cccgtgcagc aaatcattac cgagttcacg
      901 gcgaaggagg cggtcttccg gcatgcgctg gacaactaca cgtggacgcg gagcgtgcgg
      961 gtgcagacga tcgacgacga tgggaaaccg gacggccagt attaccaggt ggtggatatt
     1021 gcgttcgacg cggacgggca tcggagagag cgggtgctgg acgcaccgga gagcagcctg
     1081 acgcgcgtga tgatgtcgcc ggcggatttc gaggacatcg aggaacggct gccgtttgtg
     1141 ctgaccaccg aggatgcgcc gcagtacgac atcacgtatg tgggcaagca gaagatcgac
     1201 gagatcgaca cgtggagctt cgacgtgaag ccgaaggtca tcgagaagaa gaagcggtac
     1261 tttcaggggc ggatctgggt ggaccagaag gatcaccaga tcgtggtgac gaacggcaag
     1321 aatgtgccgg acgatacgcg gccggggcat gaggatttgt cgacgccgtt tgtgacgtat
     1381 cggcagctgg tggacggcaa gtactggttc ccggtgtaca cgcatggcga tggagtgctg
     1441 cactttaccg ccgggcatgg ctatctgagc gacaacgtgc acctgcgcga gacggtgaaa
     1501 tacacgaact accacgagtt ccacaccagc attcgcgtgc tgtacaacgg gcaggacatc
     1561 acggggagtc cggagaaggg aacggcggga caggggcagg cgccggcacc ggcgccgaag
     1621 cccgaggatc cgaagaagcc gcagtgaggg cggggctcag gccgcagggc tgaggtcgcg
     1681 ggtctcaggt cacctggctc aggcgagatt gcggccgagg gaaacagccc tgtcgcgggt
     1741 gcggctatcg atctgctgat cggggctgac agcggagcgg ccgatccaga gcctggcgga
     1801 aggggcggcg ccgagcagcc tggctgtgag tttgagggag ctggtcgccc cgctggcgaa
     1861 cgggatcatc gcgccgggca ttccggcgga gcacacaagc acggccttct tgtggagaat
     1921 ccgcgaacgc agcttcgggc cgtgattcct gccccacggc cagtacgcgg cgaagccgag
     1981 aaggcgctcc ataaattttc ggaagaccgc ggtgacgttg tagtagttga ccggcgagcc
     2041 gaggacgatg gcattggcgt cgtcgacggc gcgcaggacc gactccatgt cgtcctgctg
     2101 gacgcaacgc ccgggggtga ggccgttttg cgcggcgcag gtgcggcagt tcctgcagaa
     2161 ctggatattt tgatcgatga ggtagatttt ggcggtctgt gcgccggcaa cgcgtgcgcc
     2221 ctcaagtacg gcatcgacgg cctgatcggt aattccgccc ttgcgatagc tgcccacgat
     2281 ggctatcact tttcccatgg cccacctccg acgcctcgga aataacatga aggttgcagg
     2341 gtgtcagtat cgtgcatcac agtaccgggg cccctgcaag ggtcccaaag cggttgactg
     2401 gaggggcgaa cggcctgttt cgctgccaga tttcgccgct cagatcgtcc tggtcgctcc
     2461 tcgctgcagg tttttgcccc tgaggaggct gggcgagtac cataaaagag ctatcctgcc
     2521 ggattccacc gctcgttggc tggaggtccg catccctctc ggaggaaata cttgccagca
     2581 gctgaacgct ttctctttac ctcggaatcc gtgaccgaag gccatcccga taagattgcc
     2641 gatcaggttt ccgacgctat tcttgacgcc tgccttgagc aggacccgta cagccgtgtg
     2701 gcctgcgaga cgctgacggc gaccgggctg gtggtgatcg ccggcgaaat cacgacgcgc
     2761 gcatacgttg attttcaggc gctggtgcgg ggcgtggtga ccgcgatcgg gtacgacaac
     2821 gcggtgtacg ggttcgattc caacacctgc gctgtgatct cgacgatcaa caagcagtcg
     2881 ggcgacatcg cgatgggcgt ggacaccggc ggcgccggcg accagggcat gatgttcggg
     2941 tacgcgacga acgagacgcc ggagctgatg ccgaccccga tttcgctggc gcacaagctg
     3001 acgcggcggc tttcggaagt tcgcaaaaac ggcaagctgc cgtatttgcg gccggacggc
     3061 aagagccagg tgacggtgga gtacgacaag aaccacaaac ctgtgcggat cgacgcggtg
     3121 gtgatttcga cgcagcatgc ggaaaccgtg agcacggaag agctgcgcgc ggacatcctg
     3181 aaacacgtga ttcagtcgac gctgccggcg gaactgctgg acgagaacac gaagtaccac
     3241 atcaacccga cggggcgatt cgtgatcggc ggtccgatgg gcgatacggg tctgacgggg
     3301 cggaagatca tcgtggacac gtacggcggc atggggcggc acggcggcgg cgcgttcagc
     3361 ggcaaggatc cgacgaaggt agaccgttcg gcggcctaca tggcgcggta tatcgcgaag
     3421 aacatcgtcg cggcaggcct cgcggagcgc tgcgaggtgc agctggcgta tgcgattggc
     3481 gtggctgaac cggtgagcgt gctggtggat acgttcggca cgagcaagct ggacgagacg
     3541 gcgctggtag cgctggtgcg caagaacttc gagctgacgc cgaagggaat tatggagagc
     3601 ctgaacctgc ggcggccgat ttaccgcaag acggcggcct acgggcactt cggacgcgtg
     3661 aacgagcccg actttacgtg ggagaccacg gacaaggcgg ctgcgctgaa ggatggcgcg
     3721 aaggcacacg cgaaggagcc agtgtcagcg aagtagtttt gcgttggaac aaaagggcgg
     3781 gccgagaggc ctgccctttt tctttgggtc agtgggtcca ctggaggcgg aggtaggcgc
     3841 tgcggggaat ccgggaggcg gcaacgtagt caatggacgg gaactcgagg tggcgtgcct
     3901 ggagcagatt cgtcgcaccc gcgctgattt cgagtttttc gcccagggtg cgtgagaggc
     3961 gcagatcgag ccgcgtgtgc gcgggaacga tgaaggccga gggattgttg agcgcgccgc
     4021 ccatcgagga agcggttggg gaattgccgt tcaggatggc gcccaggctc cattgcggcg
     4081 ccgggctcca gaacccgcgg acatcgaagg cattgcgagg cgctgcccag tcctggttgt
     4141 tgagcggcac gtcggtgggg gtcccgtgat cactgaagcg gttctgcatc caggcgtagc
     4201 tgccctggaa gtgcagcgtg gtgtcaggat tccaaagcag tgaaacttcg gcgccctgcg
     4261 aatcggagga gagagcgttg aggtaggtgc agggcaggac gatcgacggg gcggggttga
     4321 aatgcaacac ggccgcgccg atcgcagtgg cgcccaggct gtaatagtga ttgacgaagc
     4381 cggcgagatc gacggaaacg ctgttgccga actgatgccg gtatccggcc tcataagcgt
     4441 ggacaatttc ggaaccgaag cgtggattgc cgctgatctg gatggtgacg ggcaaaccct
     4501 ggataacacc gccggcaaag tcgaggcggt catcgcggtc gatgtcggaa ggggtacgca
     4561 cggcgcgaga aaccgaggcc cacagcgttt gcgcgggaga gagctttgcc agcaggcggg
     4621 ccccgggctg gtattcgaag tgagagtaag tgttccagag aagcttggcg cccgcagtga
     4681 gagtgagccg gcggggaagg agggcaatct cgtcctgcag gaagccgctg gagagatgaa
     4741 tgacgtggtc cgggtgctga aacataggct cggcagcggc ggctacgtca gaggtccagc
     4801 gatacccggc tccccacacg agattctggc gcgttccgag cgagtggcgg tcctgcagat
     4861 ccaggtcgaa gctgtgcatg tggacggatt gttcgatctc gaaacggcgg tcgtcgcgca
     4921 ggtacgcctg aagaatggtc gaagaagatg cgccgcgcgt gtgctcccag cggacagagg
     4981 caaagccacc agcggcgtga atcggcgcga gggtggaggt ggtggcggcg tagtcgaagt
     5041 tcacccgctg ctgctccgcg ttgtcgtaaa gatccgcctg aagggtgatg ctatcccgga
     5101 gtcggggaga ccagtcgagg cggatgccgg cgcgttcaat gttggcggtg tcgccggccg
     5161 atgcgccatc ggcggcgtcg agcgcgtcgc ggtggagata ttttccgtag aggcgataat
     5221 cggcagaggc gccgaggtgt ccgccgtagc gggcgacggc ttcctgatcg atgcggccgc
     5281 cttctgccac gaccagggct ccctgcgtgt cctgggcctt gcgagtcacg atgttgatga
     5341 cgccgttgac ggcattggct ccccagaggg tcgcgccggg accgcggacg acttcgatgc
     5401 gctcgacgtc ttcaagcagg acgtcggtct ggtcccagaa gacgccggag tagagctggt
     5461 tgtagacggt gcggttgtcg atcatgacca gcatcttgtt ggcgaagcgg ttgttgaatc
     5521 cgcgggcgct gacggcccat ttgttggcat cgatctgggc cacttcgacg ccggggacca
     5581 tgcggaggac ttcgggaatg gaggtggcac cggaacggtg aatgtcgtcg cgggtgatga
     5641 caaagacagc ggcgggagtg tcgaagaggg gctcgtcgtg acgtgagacg gaactgacct
     5701 gaacattggt gagctgctcg agggtcatgg agaacagatc ctgggcgccg ggctttttcg
     5761 cggcgatcgc agagggcgcc gtcgcgcccg ccaggaggag cgccgatgct gcaaggatcc
     5821 aacagttttt gtgggcggtc atttttggat acctgagtcg gagatcgctg gcgcggagcc
     5881 aacgccggca cctgggcggc ctccgatgga ggtatcggca ggattgggga cgacttcagg
     5941 gtgggagggc ggagggcgag gctcgccgga ggttagaacg cactactctc cggtggactt
     6001 agcatggtga gcggatgcga aggttcgtga tttttgcggt gatttgtggc gtagccctgc
     6061 tgtgtcgcgg tcaggagacg gcgatccggg cagatcggat cgtgatcgtg aaatcggcgc
     6121 acaggatgac gctgatgagc cggggacgcg agatcagatc gtacaaggtg gcgctgggcg
     6181 atccgatagg gccgaaggtg cagcgaggcg ataagaagac gccggagggc gtgtacttcg
     6241 tcgatgggaa gaatgcgaac agtttgttcc atcgggcgct gcacttgtcg tatccgaacg
     6301 cggcggaccg ggcgcgggca cggaagatgg gcgtgaatcc gggcggggac attgagattc
     6361 acggcttgcc aaggcagtat gcatggatgg gcgcggcgca tcggtcgatc gactggacga
     6421 cgggatgcat tgccgtgacg aatgcggaga tcgacgagat ttgggaggag gtgcgggtgg
     6481 ggacggcggt ggagattcgg ccttgaggga ttgaatcggc cgttgaaact ggcgtttcgc
     6541 aggtttctgg tgaggacgtt tctttccggg cagtctgaac atatttttgg ataaagagaa
     6601 gggaagaatt gcgggagttt tccaaaacgg tccggggcac ttacttttga gtttgtcctg
     6661 attggatttt ggttgaggta ctgggtgcag aagctcaagc ttcttgctgt ggggtgaacc
     6721 tggccctcgc aatgtttttg gggtggcggg ttttttccgg gcttttcctt cgcggtcccg
//
LOCUS       DASZYN010000403         3490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_918186, whole genome shotgun sequence.
ACCESSION   DASZYN010000403 DASZYN010000000
VERSION     DASZYN010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918186"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VHE33_10320"
     CDS             complement(<1..221)
                     /locus_tag="VHE33_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97894.1"
                     /translation="MSFVLTNELERAAKRTLPEPMGELSANVLEHLRRGCFQRGLCLV
                     VAVTSVASGLEVGYEHYKGSYSNPVMYTPV"
     gene            complement(218..1336)
                     /locus_tag="VHE33_10325"
     CDS             complement(218..1336)
                     /locus_tag="VHE33_10325"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HVT97895.1"
                     /translation="MLQWKGRIELQKGSIEEAGVSAYTIPTDAPEADGTLSWDSTTLV
                     LAELRCGGITGIGYTYGTAAVAILARHLAEKCLLHQAACDIPALHASLLRQVRNDGNV
                     GIGAMAVSALDIALWDLKAKLLGRSLVDLLGAADTRVEAYGSGGFTSYSDKQLAKQLS
                     GWVKDGIRSVKMKIGTEPERDPERVRVARKAIGPDTALFVDANGAYTAKQSIALADRF
                     AESGVTWFEEPVSSDYPDDLRRVRDAVPAGMDVAAGEYGYSPAYFRHMLASQAVDVLQ
                     LDATRCRGFTGFLAGAAIAASFGCPMSAHCAPSLHMHVARAVPGFRNVEYFHDHVRIE
                     KMLFDGFIPARNGFLEPDRARPGLGLIFKRKDAERFAV"
     gene            complement(1337..3079)
                     /locus_tag="VHE33_10330"
     CDS             complement(1337..3079)
                     /locus_tag="VHE33_10330"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVT97896.1"
                     /translation="MDASDVLVERLIAWGVDTIFGLPGDGINGVFEALRKQQERIRFI
                     HVRHEEAAAFMACAYAKFTGRLGVCIATSGPGGIHLLNGLYDAKMDGMPVLAITGMQH
                     SDLIGTLTQQDVALDKLFIDVTRYNERVMSGAHMESVVDLAIRTALETRGVAHITIPV
                     DVQVQSVKKGRSERNPPHHTAATPAFAEHMPSQVDLEHAADVLNRGQKIAILAGQGAL
                     RATDELEQVAELLGAPVIKALLGKAAVPDDSPYTTGGIGLLGTEPSQEALEDCDTLFI
                     VGSSFPYIEFLPEPGSVKCVQIDIDPQRISLRYPADVGLVGNSRTCLRALIPLLKPHS
                     KGFLKKAQRAKQDWEKLMEERGTLPDKPMKPQVVGWELGKRVPSNAIVTSDSGTITTW
                     WARFVPARRGQMHSCSGNLATMACGLPYAIAAAVAYPERPVFCIIGDGGLSMLMGELI
                     TIAAYKLNIKIIVIKNNTLGQIKWEQMVFLGNPEYACDLFPADFVTIARGAGIGAMRI
                     EDPATCGAQLDEALSTSGPMLIEAVVDPFTPPMPAKVKAGQALKLAESLIRGEPNRMK
                     IALTTAHDRVRELV"
BASE COUNT          682 a         1043 c         1066 g          699 t
ORIGIN      
        1 accggtgtgt acattaccgg attgctgtag ctgcccttgt aatgttcgta accgacttcg
       61 aggccgctgg ccacgctggt gaccgcgacg accaaacaga ggccgcgctg aaaacagccg
      121 cggcgaagat gttccagcac attcgccgac agttcaccca tcggttccgg tagagtgcgt
      181 ttcgccgcgc gctcgagttc gttcgtgagt acaaagctca tacggcaaaa cgctcagcat
      241 ctttgcgttt gaagatcagg cccagccctg gacgcgcgcg gtccggttcc agaaagccgt
      301 tccgggccgg gataaaccca tcgaagagca tcttttcaat gcggacatga tcgtggaagt
      361 attcgacgtt gcgaaaaccc ggcacggcgc gggccacatg catgtgcaga gaaggcgcac
      421 agtgcgctga catggggcag ccgaagcttg cggcaatcgc cgctcccgcc aggaagccgg
      481 tgaatccacg acaccgggtc gcgtctaact ggagaacgtc gacggcctgg ctggcgagca
      541 tgtgccgaaa ataagcagga ctgtagccgt attcgccggc ggccacatcc atgcccgcgg
      601 gtacggcgtc gcgcacgcgc cgcaggtcgt cggggtaatc cgaactgacc ggctcctcaa
      661 accaggtgac gccgctttcg gcaaatcgat ccgccagtgc gatcgactgc ttcgccgtgt
      721 acgctccatt ggcatcgaca aacagggctg tgtctggacc gatcgctttg cgcgccacgc
      781 ggacgcgttc cggatcgcgc tcgggctcgg taccaatctt catcttcacg ctgcgaatgc
      841 cgtccttcac ccagcctgac agctgcttcg ccagctgctt gtcggaatag gaggtgaatc
      901 cgccgctgcc gtaggcctcc acccgcgtgt ctgcagctcc cagcagatct accaaactgc
      961 gcccgagcag ctttgccttg agatcccaca gagcgatatc gagcgccgac acggccatgg
     1021 ctccgatgcc gacattgccg tcgtttcgaa cctgacggag cagggacgcg tgcaatgcgg
     1081 ggatatcgca cgcggcctga tgaagaagac atttctcggc gagatgacgt gccagaatgg
     1141 cgacggcggc agttccgtag gtatagccga tgcctgtgat tccaccgcag cggagttccg
     1201 ccaaaaccag cgtggtcgaa tcccagctca gcgtaccgtc agcttcaggt gcatcggtgg
     1261 gaatggtgta tgccgatact ccagcctctt cgatcgatcc tttctgcaac tcgatccgcc
     1321 ctttccactg cagcaactaa accagctcgc gcacacgatc atgcgccgtc gtcagagcaa
     1381 ttttcatgcg gttgggttcg cctcggatga gggactccgc cagtttgaga gcctggcctg
     1441 ccttcacttt tgccggcatc ggcggcgtga atggatcgac cacggcctcg atcagcatgg
     1501 gtccgctggt gctcagcgcc tcatcgagtt gcgccccgca ggtggcgggg tcctcgatac
     1561 gcatggcccc aattccagcg ccccgtgcga tggtcacgaa gtcagcgggg aacaggtcgc
     1621 aggcgtattc cggattgccg aggaagacca tctgctccca cttgatctga cccagcgtat
     1681 tgttcttgat gacgatgatc ttgatgttga gcttgtaggc ggcgatggtg attaactctc
     1741 ccatgagcat gctgagcccg ccgtcgccga tgatgcagaa gacggggcgc tccgggtaag
     1801 ccacagcggc ggcgatggcg tagggcagac cacaggccat ggtggcgaga tttccggagc
     1861 agctatgcat ctggccgcgc cttgcgggaa cgaaacgtgc ccaccatgtg gtgatggttc
     1921 cggaatccga cgtgacaatg gcattcgatg gaacccgttt acccaactcc caaccgacaa
     1981 cctgcggctt catcggcttg tcgggcagcg taccgcgctc ctccatcagc ttttcccagt
     2041 cctgttttgc ccgctgcgct tttttcagga aaccctttga atggggcttg agcaggggaa
     2101 tgagcgcgcg caggcacgtc cggctgttgc cgaccagtcc aacgtcggcc gggtagcgca
     2161 gcgagattcg ctgcggatcg atgtcgattt gcacgcactt caccgacccc ggctcaggga
     2221 ggaactcgat ataaggaaac gatgagccga cgatgaacag cgtgtcgcag tcttccagag
     2281 cctcctgcga tggctcggta cccagcaggc cgatgccgcc ggtagtgtag gggctgtcat
     2341 cgggaaccgc tgcctttccg agcagagcct tgatgacggg cgcgcccaga agctccgcta
     2401 cctgttccag ttcatcggtg gcgcgcaacg ctccctggcc cgcgagaatc gcgatcttct
     2461 gcccacgatt caaaacatct gcggcgtgct ccagatccac ctgcgagggc atgtgttcgg
     2521 cgaaagcagg cgtggcggcg gtgtgatgcg ggggattgcg ctcggagcgg cctttcttga
     2581 cggactgcac ctgcacatcg accggaatgg tgatgtgcgc gacgccgcgc gtttcgagtg
     2641 cggtgcggat ggccagatcc acgacgcttt ccatatgggc gccgctcatc acgcgttcgt
     2701 tataacgggt cacgtcgatg aacagcttgt cgagcgccac atcctgctgg gtcagcgtac
     2761 cgataaggtc cgagtgctgc atgccggtga tcgccagtac cggcatgccg tccatctttg
     2821 cgtcatagag gccgttcagc agatggatgc cgcccggacc tgacgttgca atgcagacac
     2881 cgaggcgccc ggtgaatttc gcataggcac aggccatgaa cgcggccgct tcttcgtggc
     2941 ggacgtggat gaaacgaatg cgctcctgtt gcttgcgcag ggcttcaaaa acgccgttga
     3001 tgccgtcacc gggaaggcca aagattgtgt ccacacccca ggcgattaag cgttcgacga
     3061 gaacatccga tgcgtccatt gatccctctt tcgggatgcc gttccttcgg cggtccagat
     3121 actggtagga cgcacacgtt gaaaatgggg tttgttgatg atgcaatcgg ctcgcagcaa
     3181 taaggctaaa aaggcgcgac gggtacacct ctcgcctggt ttgcgccaca aaccgaggca
     3241 agtttggggc agcacgacag gtgcaggttg tgaagttcgg cgcacatcgc ggagaggtaa
     3301 aacacagtgc gattcgtgat ctttgccgct ttacgtcacc tcgtcagacg gcaattggct
     3361 gcgagttcgc gtgctatagt agggagagat tcggcggaga tttttgtctg cggggccacc
     3421 cgcgcgcgcc ggactgaacg tggacgcgta gctcaattgg cagagcattc gactcttaat
     3481 cgacaggttg
//
LOCUS       DASZYN010000404         2671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_725688, whole genome shotgun sequence.
ACCESSION   DASZYN010000404 DASZYN010000000
VERSION     DASZYN010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725688"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VHE33_10335"
     CDS             complement(<1..226)
                     /locus_tag="VHE33_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97897.1"
                     /translation="MQRAGDFRDGTASPERVSDLSVLFVLVFVFAVQMAHAGGPRWVA
                     GSSYFDSSVQGRPIVWANGQVTYSTDLGNLS"
     gene            453..1388
                     /gene="trxB"
                     /locus_tag="VHE33_10340"
     CDS             453..1388
                     /gene="trxB"
                     /locus_tag="VHE33_10340"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HVT97898.1"
                     /translation="MADNIRDTVIIGTGCAGLTAAIYSARANLHPLVLEGHEPGGQLS
                     ITTLVENFPGWPEGIQGPELIENMRKQASRFGAEFRLGHLATADLTKHPFALNLGHET
                     IHTRTLIIASGASARWLGLPSEQALIGHGVSSCATCDGFFFTGKEIAVIGGGDSAMEE
                     ALFLTRFASKVTLIHRREQFRASKIMLDRAIAHPKIQFVTNTQVKEVLGVEQKEVTGL
                     RLLDATTGVESELSISGMFLGIGHIPNAAMFAGQLDLDEDGYIKTHNNVFTRVPGVFA
                     CGDVQDRRYRQAITAAGSGCMAALEVEKYLEEHGH"
     gene            complement(1454..>2671)
                     /locus_tag="VHE33_10345"
     CDS             complement(1454..>2671)
                     /locus_tag="VHE33_10345"
                     /inference="COORDINATES: protein motif:HMM:NF024686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HVT97899.1"
                     /translation="PYVAPPTISPAGGTFKYVQTVTIADTMAGAAIYYTRDGSTPTAS
                     STPYTGPITVTGGLNVTAIAILNGISSQASNQKYTVNDISGLMSTVAGSGKQKYNGDG
                     INALGAAIYPTGVAFDSHGNLFIADFGNLRVRMVTPTGIISTVAGNGNLCPFVEGIAA
                     TDSCLRPMDVAVDKNDNLYISIDDRICKVTTDGVITTFAGGAEESGAAGVGDGGPATG
                     ASLFGPDEIAFDTNGSLYIADTTHGLIRKVTPDGIITTVAGGNTANNNGDGGPATDAQ
                     LRHPVGVAVDSKDDIYISDDYDSTVRKVTPDGIISTIRVPTLINPGGLAFDNRGGLLY
                     VTDTRQSAIFVLTPDGIWYRAAGNPPSNGFLGDGGDSQNATLTLPNSVGLDPAGNLYI
                     ADTFNYRVRKVTF"
BASE COUNT          583 a          838 c          722 g          528 t
ORIGIN      
        1 cgctcagatt gccgagatcc gtggaatagg tgacctgccc attcgcccac acaatcggtc
       61 gtccttgaac cgacgaatcg aaataactcg acccagctac ccagcgcgga ccgcccgcgt
      121 gtgccatttg cacggcaaaa acgaacacca ggacaaacaa aaccgaaaga tccgaaactc
      181 gctcgggtga agctgtcccg tcccggaaat cgcctgcacg ctgcatcgcg gggccctccg
      241 gaagcagctg aaaccaaacg cctcgccgct cccgttttca ttcccgcaca ccgcacggca
      301 catcagcaca agaatcgaat cggcatcgaa ccgcatcatt tcagttcggg acagccgcag
      361 atcgtggtgc cattttggca ccgcacgacc gctccccggc gaggacgcca gattccggtc
      421 ccatcgcagg tacaatttga aagttcagac tcatggcaga caatattcgc gataccgtca
      481 tcattggcac cggttgcgcc gggcttaccg cagcgatcta cagcgcccgc gccaatcttc
      541 atccgctggt tctggaaggg catgagcccg gcggtcagct ctccatcacc acgctcgtcg
      601 agaactttcc cggctggccc gaaggcatcc agggacccga gctgattgag aacatgcgca
      661 agcaggcctc ccgcttcggg gccgagttcc gcctcggtca tctggccaca gccgacctca
      721 caaaacaccc gttcgcgctc aatctgggcc acgaaacgat ccacacccgc acgctcatca
      781 tcgccagcgg cgcctccgcg cgctggctcg gtcttccctc cgagcaggcc ctgatcggac
      841 acggcgtcag ctcctgcgcc acctgcgacg gcttcttttt caccggaaag gaaatcgccg
      901 tgatcggcgg cggcgactcc gctatggaag aagccctctt tctcacgcgc ttcgccagca
      961 aagtcaccct catccacagg cgtgagcagt tccgcgcatc gaaaatcatg ctcgatcgcg
     1021 ccatcgccca ccccaaaatc cagttcgtca ccaacaccca ggtcaaggaa gtcctgggcg
     1081 tcgaacaaaa agaagtcacc ggcctccgcc tgctcgatgc gactaccggc gtggaatccg
     1141 agctcagcat cagcggtatg tttctcggca tcggccatat ccccaacgcc gccatgttcg
     1201 ccggacaact cgatctggat gaagacggat acatcaagac ccacaacaat gtcttcacgc
     1261 gggttccagg cgtcttcgcc tgcggcgacg tccaggatcg acgctatcgc caggccatca
     1321 ccgcagcagg aagcggttgc atggccgctc tcgaagtcga gaaatatctc gaagagcacg
     1381 gccactaagg gtcattgatc ccgctgagat atcaacgcgg ccttttggct ccggtcggaa
     1441 aaaccgtctg atctcaaaag gtgaccttgc ggacgcgata gttgaacgtg tccgcaatgt
     1501 acagatttcc ggcgggatcg aggcccaccg aattcgggag agtcagggtt gcattttggg
     1561 agtcgccacc gtcgcccaaa aatccgttgc tcggcggatt tccggctgca cgataccaga
     1621 tgccgtcagg agtgagcaca aagattgcac tctgccgagt gtctgtcaca tacaacaaac
     1681 cgccccggtt atcgaatgcc agtccgccag gattgatcaa tgtgggaact cgaatcgtgg
     1741 aaataatccc gtccggagtg acctttcgga cagtgctgtc gtaatcgtcc gagatataga
     1801 tgtcgtcctt tgagtcgacc gcaactccga cagggtgccg caattgcgcg tccgtcgcgg
     1861 gacctccgtc gccattgtta ttggcagtat ttcccccggc taccgtagtg atgatgccat
     1921 ctggagtgac ttttctgatg aggccgtggg tagtatcagc gatatacaga ctaccgttcg
     1981 tgtcaaaggc gatctcgtcc ggcccgaaaa ggcttgcacc ggttgccggg ccaccatcac
     2041 cgacaccggc ggcaccgctt tcttcggcgc caccggcaaa ggtcgtgata actccatcag
     2101 tcgttacttt acaaatgcga tcatcgatcg agatgtagag gttgtcgttc ttgtcgaccg
     2161 cgacatccat gggacgaagg caggaatcgg tggcggcaat cccctccaca aatgggcaca
     2221 ggttcccgtt tccagccacg gtggaaatga tccccgtggg cgttaccatc cgaacccgta
     2281 gattgccaaa atcggcgata aagagattgc cgtggctgtc gaacgcgaca cccgtcgggt
     2341 agattgccgc accgagtgcg ttgatgccgt ctccgttata tttctgtttg ccactaccgg
     2401 caacggtgct catcagaccg ctgatatcgt tgaccgtgta cttctggttc gatgcctggc
     2461 tgctgatccc gttgagaatt gcaatcgccg taacattcag accgccggtt acggtaattg
     2521 gacccgtata aggtgtagac gacgcggtgg gcgtcgagcc atcccgagtg tagtaaatag
     2581 ccgcgcccgc catcgtatcc gcgatggtta cggtctggac gtacttgaaa gtcccgccgg
     2641 ctggagagat ggttggggga gcgacataag g
//
LOCUS       DASZYN010000405         1959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1173748, whole genome shotgun sequence.
ACCESSION   DASZYN010000405 DASZYN010000000
VERSION     DASZYN010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173748"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VHE33_10350"
     CDS             <1..187
                     /locus_tag="VHE33_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97900.1"
                     /translation="THEMQLISLHPGVTLEEVEAAVAWPLKRVENLTTTPAPTARELH
                     TIRTEVDPTGMYSKGQS"
     gene            complement(200..1381)
                     /locus_tag="VHE33_10355"
     CDS             complement(200..1381)
                     /locus_tag="VHE33_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97901.1"
                     /translation="MSPLFRRGTDLARSVDHLAEMLEKREEDGAAWQRAMGTVLKEAE
                     RAAPEEIRQTQERLAEMMVKMPLMLSALFALGSGALIERGADAEIPARAIFARTQDAL
                     LLATRFGIACQEEARRHHPDMPQKQANDKEEEEEEFAVDDIEVCVEAYGAELAERMGE
                     EAEGWQALSPLSTAALAIATRSAAQRALARDNAAFMQAMYECPAETSLHCLRVALNLL
                     EKEKVVVLHPALQRGYLIRISNIALNFELHTLLADALIGDPAQGWLAGQRPDPRVVAA
                     AKDGPFPMPGDRRADSFPHAEGAFNLWNWRGLQADGQLSQEGIGNTEHWIWNEGQPID
                     IQPFEGTRVILLGPAPYQRSWNAGRFFPHLSSELEVVEKLSEQQVNDWLTRIVAAVQG
                     R"
     gene            complement(1626..>1959)
                     /locus_tag="VHE33_10360"
     CDS             complement(1626..>1959)
                     /locus_tag="VHE33_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97902.1"
                     /translation="TLMDYTMPIASQLPAFETAFVETPTPINPLGIKGIGESGTTGGP
                     PAIVNAALDALAPLGIRVLDMPLKPEKIWSKIAAVRAGQPPEVDPDVPAIFSTLQANQ
                     ESGTSTFA"
BASE COUNT          379 a          612 c          567 g          401 t
ORIGIN      
        1 cacgcacgaa atgcagctca tctccctgca tcccggtgta accctggagg aagtagaagc
       61 cgccgtggcc tggcccctca agcgcgtcga aaatctgacc accacgcccg cgccaaccgc
      121 ccgggaactt cacaccatcc gcacagaggt cgatcccact ggcatgtact caaagggcca
      181 gagctagggt gaaacgcgct tatcgcccct gaacggcggc gacgattctg gtcagccagt
      241 cgttgacctg ctgttctgac agcttctcca ccacttccag ctcgctgctg agatgcggga
      301 agaagcggcc cgcgttccat gagcgctgat aaggcgccgg accgagcaga atgacgcgcg
      361 tcccctcgaa gggctggata tctatcggct ggccttcgtt ccagatccaa tgctcggtgt
      421 tgccaatgcc ctcttgtgag agctggccat cagcctgcaa gccccgccag ttccagaggt
      481 taaaggcacc ctcggcgtgg ggaaagctat cggccctcct gtcgccgggc atggggaagg
      541 gaccatcttt ggcggcggcc actacgcgcg gatcggggcg ctgtcccgcc agccagccct
      601 gcgcgggatc gccgatcagg gcatccgcca gcagcgtatg cagctcaaag ttgagcgcga
      661 tgttgctgat gcgaatgaga tagccgcgtt gcaaggcagg atgcagaacg accaccttct
      721 ctttctccag caaattgagc gccacccgca ggcaatgcag gcttgtttca gcagggcact
      781 catacatcgc ctgcataaat gccgcgttgt cccgcgcaag cgcgcgctga gcagcggaac
      841 gggtcgcgat agccagtgcc gccgttgaaa gcggactcaa ggcctgccat ccctcggcct
      901 cttctcccat gcgctcagcc agctctgctc cataagcctc aacgcagacc tcgatatcat
      961 cgactgcgaa ctcctcctct tcttcctctt tatcgtttgc ttgcttttgc ggcatgtccg
     1021 ggtggtgccg cctggcctcc tcctggcagg cgataccgaa gcgagttgcc aggaggaggg
     1081 cgtcctgtgt gcgcgcgaaa atagccctgg cgggaatctc ggcgtccgcg cctctttcaa
     1141 tcagcgctcc agaacccaga gcaaaaagcg cggagagcat aagcggcatc ttcaccatca
     1201 tctccgccag ccgctcctgg gtctgccgaa tctcctccgg ggcggcccgt tccgcctctt
     1261 ttaaaactgt tcccatcgcg cgctgccacg ccgctccgtc ctcttcccgc ttttccagca
     1321 tctcagccag gtgatctact gaccgggcaa gatcagtccc acgacgaaat aatggactca
     1381 tgctacctca ttcttccctg atttcagcaa cgaaatcaca tcttttttgc aggcctgctc
     1441 tatcattgga taagcttgcc tgtctataca ttcttctttt tagggcaaaa gagagcggtt
     1501 ataatgaatg agctgccctg tccgaacctg tgcggcgctc tttcgcgaag cgaagcgaaa
     1561 aagcgtcgaa taggttcgga tagggcagcg aaggaagctg cgccgtcctt tctggcaaaa
     1621 ggcgctcagg cgaaggtgga cgtgccgctt tcctgattgg cttgcagggt actgaagatg
     1681 gcgggcacat ctggatcgac ttccgggggc tggccggcgc gcacggcggc gattttactc
     1741 cagattttct ccggcttgag cggcatatcg agcacgcgaa tgcccagcgg cgccagggca
     1801 tcgagggcgg cattgacgat ggccggtggt ccgccagtgg tgccgctttc gccgatgcct
     1861 ttaatgccga gcggattaat gggcgtgggc gtttcgacaa aggccgtctc aaaggcgggg
     1921 agctgactgg cgataggcat ggtgtagtcc atgagcgtg
//
LOCUS       DASZYN010000406        18652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_918508, whole genome shotgun sequence.
ACCESSION   DASZYN010000406 DASZYN010000000
VERSION     DASZYN010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 18652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18652
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918508"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /locus_tag="VHE33_10365"
     CDS             <1..1428
                     /locus_tag="VHE33_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97903.1"
                     /translation="GLSTNTNPVAAAPDLYNVTGMIYSDQYALKDPTLCAVVAVRQNS
                     TNSWYIYFAPYDATESITLLKDGIVTVPEPYSPRWLGQIGHVSGINYTYALPGGPDQL
                     TCVLQVPPDYRTDAMNPGRILTAYRGGGCIWEGILTEPTPSPTGWTLTANGAGTYGTN
                     FAAWWQIGDALSWNADRVLEYAIARGLRWNLPGINNPPGLYLGPVQESGSMTITDFMN
                     LLCTGGALTWQVTQPAGASTFPPRPWDIRVFPLPSDSSGNPIESAPSNQLSSQLFTFD
                     RWSRSDLSATQSRRPPDLYIVNTNPMGRTITADYNTVVVYYQVTADTTATSTKAATSA
                     TYQTTYADIPQSVAQHGRLEYFLDVSSAGVMTQPQASQIAVNVLTKYVRANFSGTFTV
                     QPGQLLNVGGFPVDLGCNWGGYVASVQVETFAYGGEVAFGPVTFQIGEYAFDDDTQTA
                     TVTPYQSARTDISSVIAQLYPGKFS"
     gene            complement(1706..1864)
                     /locus_tag="VHE33_10370"
     CDS             complement(1706..1864)
                     /locus_tag="VHE33_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97904.1"
                     /translation="MAGRKKKSEDRPNCGAICPKCRGKCECKLYQEHPGDHFSYKGMG
                     GCGHQWPQ"
     gene            complement(2201..3049)
                     /locus_tag="VHE33_10375"
     CDS             complement(2201..3049)
                     /locus_tag="VHE33_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97905.1"
                     /translation="MSFTLAFDATSANFSRLPGGSQVAGYDSGTPDIRWSAAQFAAHP
                     GALHIDQDASGSITTSDILDVERGAATLGHIVSWVLEARAAWNGIRRPGQRHPAIYAS
                     ASNITPVVNTLVSGGVQSGVGLWVANWDLSEVQAIADVMNASGPFPVIGVQFASSDAL
                     GYDTDIFSEQWLDDVSGAAWVFTPVRSLTAKFGKSSIQVSGYSPTPPKPLGVGSYEMT
                     VYPEGTGGPVLATYPRFFPKGSSSHFSFQGGSVPPGNWTVGVRAVATDGGHAGPWALA
                     SGTVTG"
     gene            complement(3323..3643)
                     /locus_tag="VHE33_10380"
     CDS             complement(3323..3643)
                     /locus_tag="VHE33_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97906.1"
                     /translation="MSCQILVKTAPHMQGHYSFLCEDPVARQEFANRADVFWYAFPED
                     DEIVWRKGGAWDAAEPVPSPVAYGGRFEYEDAEDLRRAHLKPVSEEDDEDDDRWLPVR
                     RQAV"
     gene            complement(3640..3780)
                     /locus_tag="VHE33_10385"
     CDS             complement(3640..3780)
                     /locus_tag="VHE33_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97907.1"
                     /translation="MAEPDPGKEPHEYCVVHGQPLDLCLLPHWCRKVMKSWGLYPDLE
                     DE"
     gene            complement(3773..4258)
                     /locus_tag="VHE33_10390"
     CDS             complement(3773..4258)
                     /locus_tag="VHE33_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97908.1"
                     /translation="MDSAIGVDPGPRTGLCFIDYAGGQVVGHTLLQVDWRSARHVLGA
                     VLANYYTETGPAGFMRRVASVEAWTEARSGSAGKPGTVTRQLVVELTEELQLFGYQVT
                     IRKAADVKPWASDRRLEKVFGKESFHGDMNHAFDAARHCIYGARQAELCPDPLARRTV
                     G"
     gene            complement(4264..4530)
                     /locus_tag="VHE33_10395"
     CDS             complement(4264..4530)
                     /locus_tag="VHE33_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97909.1"
                     /translation="MNGMTVESLEDLEELLTGPPHPCETITGTTVCARPADFRISWDC
                     DGCAVRHIAWICSKCWTLLRIRDTIGADCVCPKTGAPLRDVTRL"
     gene            complement(4527..4691)
                     /locus_tag="VHE33_10400"
     CDS             complement(4527..4691)
                     /locus_tag="VHE33_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97910.1"
                     /translation="MRNRCHHRSRLGLPCFRTWVYDYWCGHHNGTCWWHTMLDNRHPW
                     NRKAYLRRMA"
     gene            complement(4688..5413)
                     /locus_tag="VHE33_10405"
     CDS             complement(4688..5413)
                     /locus_tag="VHE33_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97911.1"
                     /translation="MLAACERSGRVATEFAKRGWEAWSCDLEPSDQLMHIWVSENFEH
                     DGVYRHHRGDVLELFEWDHPVNSRVKFGLEPVWDLVIAFPPCTDISSAGARYWKEKDI
                     SQGGDGRMQWGAKLFMDMIYAPAPHVAVENPMGKMNQWYRYPDQVVEPWWFGDPYSKK
                     TCLWLRNLPMLVADNPVAPVGRVATGGGSWRTDRNAQRVLMSKYEDSRGRVNREYMRS
                     LTFEGFARAMADQWGPYIEKEMA"
     gene            complement(5424..5579)
                     /locus_tag="VHE33_10410"
     CDS             complement(5424..5579)
                     /locus_tag="VHE33_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97912.1"
                     /translation="MSGPQDPLNGVGQLAVITHELFLSLETAGFTETQALFLTGQWLQ
                     AAVARAS"
     gene            complement(5576..5731)
                     /locus_tag="VHE33_10415"
     CDS             complement(5576..5731)
                     /locus_tag="VHE33_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97913.1"
                     /translation="MTLSPAWLSDLRQVSADSNLTWSETADGIVYLAGYEVTVGEQYG
                     PPSLEKI"
     gene            complement(5796..6152)
                     /locus_tag="VHE33_10420"
     CDS             complement(5796..6152)
                     /locus_tag="VHE33_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97914.1"
                     /translation="MRVTVASKRRRNKNRQHSFRRRKDYPRAVQAGMHRCPACGKWCY
                     RTRADAESTVGKLHPGATVHYYTCADTGFWHFTSMTAEQMARIRARRAFEETGDEYPG
                     DAYPQEEDAYPEEEAV"
     gene            complement(6183..8021)
                     /locus_tag="VHE33_10425"
     CDS             complement(6183..8021)
                     /locus_tag="VHE33_10425"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVT97915.1"
                     /translation="MATVFGELDPSGQFIIVQVTDAGDHELNQAAAVMRAMTPNFNPV
                     SETALQVPLSWAAVTQLACAFTGAAGWDGRPGLKWVPGAELSAWVMVEIIRRSCQGDV
                     TGKPPQLTPMAHQSAAAVAIGMNGRFLLADEMGTGKSGSYLMGLAELEARNCEPWPAL
                     IVTPAGVVDTLLEEIPQWYPDWKAVAYRGTGRGKYIRSNAQLLVMGYETMRNDTGSEA
                     KPGPLMRMRAGSVVFDECHALCNYNSLQSREARRLAKHVRNVVAGSGTPITRNVAGFW
                     PVLNSMYPASYPARDRYKSHYCLSRRTSGPQYGNGDADIIGLDPVREPEFRTAMQGTF
                     RRVALDDVVDMPPKTYETRWIEIPPAWRAAYNQMEEDMLAELPDSMTPLEAQVAVVKM
                     MRLRQLACSACDVAVTRETEGNPRSPKYGEEVTHTEVTLREPCWKGQALVDLLDEIHQ
                     AEGEFDGLGRRHGHTVGSRPVIAFAEYAQLTRLAGKMAAKAGYQVAFIDGDVKPADRN
                     RNRLAFQNNELDLLCVTTGAGGVGLNLTASGDVVFLARPWGYVPAVQSERRAWRKGQD
                     KPVRIYDFVAKNSVESRVRARLQEKAENLADLVQDPRIARSFLGAS"
     gene            complement(8126..8263)
                     /locus_tag="VHE33_10430"
     CDS             complement(8126..8263)
                     /locus_tag="VHE33_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97916.1"
                     /translation="MPRWLWWIIGALVVWWIIQNPAAAGNLVHQVFTSAGLFASHSTQ
                     K"
     gene            8483..8638
                     /locus_tag="VHE33_10435"
     CDS             8483..8638
                     /locus_tag="VHE33_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97917.1"
                     /translation="MFGILAFILFVIAAILGWTGSEAQHVLAIIAAGLAALTAEVVFA
                     WRPWVHP"
     gene            complement(8887..9390)
                     /locus_tag="VHE33_10440"
     CDS             complement(8887..9390)
                     /locus_tag="VHE33_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97918.1"
                     /translation="MRKFIAPVLIAAGAALVAGCQSSSSSTVAVQADTTRPSMAASAS
                     VPADTPQAVTPSTSGTYEGACAYTLGDNPAGGTAVATGDIDVTNTGNIGEVIKLKISW
                     PQQGFSPLTRTSTVRLAKGQEQDVQFNHPLTSDQLDNLQNWQTSHGFKSGCTYNGTIV
                     STFGTAS"
     gene            complement(9481..10350)
                     /locus_tag="VHE33_10445"
     CDS             complement(9481..10350)
                     /locus_tag="VHE33_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97919.1"
                     /translation="MLKRILASLAVVTAAGAGTLATTGWASATPSTATATTHLTNRPD
                     SGYSGNTWADDNLVRTVTVRLVGPDTVSGLYDYTATLHDTGTASALTGATSPGSQAVA
                     IKGAPVARVSGSGTYTFTANTSQFSAKDVPFKLSGSAGPTDTWPEYFFAPGTQFTGMG
                     ISNWTWVYTDSRHCQTWTDAYNGSQATSGDITGVSACPVPAPKPVIRHLHTDWVSSHS
                     AEVSWNQTVTSTDLVTVTGPGLGRHGWTFATHNDKADFGKLRPWSRYQVTVQPTVNGK
                     PAGHSASISFRTR"
     gene            complement(10493..11488)
                     /locus_tag="VHE33_10450"
     CDS             complement(10493..11488)
                     /locus_tag="VHE33_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97920.1"
                     /translation="MFKRTIFAAAGLAGAATLALSGVAHAGVNYNNVRYTQASATSSV
                     AGYEAYSNHGDLFQFTHLEANLGINSHGLAVLPAVSLSGLGSSESDTTVNVTSQKGAI
                     QGGAGIQLCNSAGYVAQVGLVNVGGGKMDVVAAAALVGDARYNGDKCENGLATGNTLS
                     GAVVLLANVPVTHAVEVGLLYDPKHAYSYGGRHHSAGTATAYAVDDMADSYSNQITIP
                     GVRSGLNFNNASAGVVADNATSTSLNEAVPDPDHASTANRLFTAAHVKVNGNSEVNGT
                     HEVQGAFQNSTAWLVSPVAATSDGTKDGLLFLNPHVFFDDHFDVDAGSTVITPAA"
     gene            complement(11598..12731)
                     /locus_tag="VHE33_10455"
     CDS             complement(11598..12731)
                     /locus_tag="VHE33_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97921.1"
                     /translation="MTYPQQPYPDPSQQPYGQPAPGYPQQPVPQAQFQQPQYPQYTQT
                     TYTQPYAPQQQGTPQQMMQPEPELPRGSLTGFFGQPAGGGGPGLTFETLGTRYVGTVS
                     RNVTAADVHVVTKMRPRPGEDPVDRHPDGRPKESMWIPLVVQPSPQFPNGEATWYVKP
                     NERAELLRAMQEAGVETDPETGFMYPPRAGDVIDITYIASKPNRGGMSDTKVKKVVYT
                     KGNGQAPVPAYVQQAQQQIQAQPQQGAQPQQWGQPSPNNYPVPGGTQFAMPGPGQPPY
                     QTVAGRLPPQQPQFQQSPPPQQPVYGQPNADWNPAAQQQPVPTPAPAPAVPLAQPGPG
                     ATGTASVSPSKPADWPDDVRFIPGLTVEQARMAQVHQIQVPGQ"
     gene            complement(13038..13295)
                     /locus_tag="VHE33_10460"
     CDS             complement(13038..13295)
                     /locus_tag="VHE33_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97922.1"
                     /translation="MVTRPEHVPSLSQVRARVCLKTLGETVDGDVFYLAADPSRVFYR
                     GDMDWDEYPFTELMSDRWMDPGEVGDGSAGQLTVVIHNTEQ"
     gene            complement(13295..13447)
                     /locus_tag="VHE33_10465"
     CDS             complement(13295..13447)
                     /locus_tag="VHE33_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97923.1"
                     /translation="MERDRPRDARVLRDAAEVVREYSTRPASFWCTVLVRVLRRAAAR
                     IEAGGW"
     gene            complement(13447..14262)
                     /locus_tag="VHE33_10470"
     CDS             complement(13447..14262)
                     /locus_tag="VHE33_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97924.1"
                     /translation="MPDAASSYMSAAPRSVNASDAWAEGYSLEIKGILTRYAQRLPRQ
                     LQVHLGPSELGHECDRQVVGKMAGAKQTNHVADVWSSVMGTAGHAFVAAAFDWDNEHN
                     GYTRWLTERRVTPDPGPDPHPGTADLYDHVYQCVVDHKFQGDSTRSRLKTQGPPMHYY
                     VQLLLYRQGYLNLGLPVNRVVIVSWPRTKSSLDEMYVWSHVPTAADDELVRSVLAQTA
                     FRQQVAELVKAGKISLMDVPAKPEDTECYFCGLYRPQARYDGQYGCPGTLVEK"
     gene            complement(14263..14646)
                     /locus_tag="VHE33_10475"
     CDS             complement(14263..14646)
                     /locus_tag="VHE33_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97925.1"
                     /translation="MDTDTDTAQITVPVRDRTVIVEVPKDSRLEQLLCIAEVTKAKAD
                     AAQDAYDELKDGILAELVALHPDLDIKGYDIPAGPMWPGFTYTWQSQFFLPAPKIREH
                     LPPVYEAFKQEKSFWRFGIKKKSKR"
     gene            15073..15396
                     /locus_tag="VHE33_10480"
     CDS             15073..15396
                     /locus_tag="VHE33_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97926.1"
                     /translation="MPAGDLFQLFGGAGAGVVGVCLIFVILFITGQISTKKQLDDAEL
                     RYQQKADECEEWKEAWRLERARGDANEATGRIVKDVMLGLRQVGKELELDVAVQAQEE
                     AHDCR"
     gene            15383..15565
                     /locus_tag="VHE33_10485"
     CDS             15383..15565
                     /locus_tag="VHE33_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97927.1"
                     /translation="MTVAEAGLEEARKARRSAAERLAVTTSEVTIPLRNLHKENHIGP
                     LLDGLIQKKAERTYRT"
     gene            15562..15924
                     /locus_tag="VHE33_10490"
     CDS             15562..15924
                     /locus_tag="VHE33_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97928.1"
                     /translation="MTQAQLIHDLDEYVIWFSFLLSVLFAPLATMMWPWWKHPWGINI
                     ISLEVAIAFALLPAWLSIAFGLRRADVYVFAWVQLASLVLVGLVIAWRLVLIWRTQRR
                     DTPSAEPAPPPRERDPAS"
     gene            complement(15921..16043)
                     /locus_tag="VHE33_10495"
     CDS             complement(15921..16043)
                     /locus_tag="VHE33_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97929.1"
                     /translation="MQRLTIRCSRKRCPHLKGIAGHCLVGGCPNDKNACPLHGD"
     gene            complement(16853..17278)
                     /locus_tag="VHE33_10500"
     CDS             complement(16853..17278)
                     /locus_tag="VHE33_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97930.1"
                     /translation="MSRGWTDNDPAKGTPHRRTREKISLLLNDLQGDSEALVAVIDKI
                     AEKHPDAVLDAVLWAADPDADLPVRRKDLGPHGNWLNELADASMRAAQDKFFLDDDGP
                     VVCVTHLRFLPCRKDAGCVYSRDETEIARVRAYQNGEGA"
     gene            17791..18120
                     /locus_tag="VHE33_10505"
     CDS             17791..18120
                     /locus_tag="VHE33_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97931.1"
                     /translation="MDHRSDDRIMAELRIMERRLNDKLEEILTAQNDIDAAVTALTGL
                     VSDIQAQSANLSTAVTAIQNEISSLQSQGVDTSALDAAVAQVADAQSALDSSVSSVSS
                     VVPPANS"
     gene            complement(18224..18297)
                     /locus_tag="VHE33_10510"
     tRNA            complement(18224..18297)
                     /locus_tag="VHE33_10510"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18261..18263),aa:Met,seq:cat)
BASE COUNT         2781 a         6261 c         6214 g         3396 t
ORIGIN      
        1 gggctgtcga ccaacacgaa cccggtcgcg gcggctcccg acctgtacaa cgtcaccggc
       61 atgatctaca gcgaccagta cgcgctgaag gacccgaccc tgtgcgcggt ggtggcggtg
      121 cggcagaact ccaccaactc ctggtacatc tacttcgccc cgtacgacgc caccgagtcg
      181 atcacgctcc tcaaggacgg catcgtcacc gtcccggagc cgtacagccc gcgctggctc
      241 ggtcagattg gccacgtgtc ggggatcaac tacacctacg ccttgcctgg tggccctgac
      301 cagctcacat gcgtgctgca ggttcccccg gactaccgga ccgacgcgat gaacccgggc
      361 cgaatcctca ctgcctaccg gggcggcggg tgcatctggg aaggcatcct gaccgagccg
      421 acaccgtccc ccaccggctg gacactgacc gccaacggcg ccggcaccta cgggaccaac
      481 ttcgccgcct ggtggcagat cggggacgcg ctgagctgga acgccgaccg ggtgctggag
      541 tacgccatcg cccgggggct gcgctggaac ctgcccggca tcaacaaccc gcccggcctg
      601 tacctgggcc cggtgcagga gtccgggtcg atgaccatca ccgacttcat gaacctgctg
      661 tgcacgggcg gcgcgctgac ctggcaggtg acccagccgg ccggggccag cacgttcccg
      721 ccgaggccgt gggacatccg ggtcttcccg ctgccgtccg actccagcgg caacccgatt
      781 gagtcggccc cgtcgaacca gctgtcgtcg cagctgttca cgttcgaccg ctggtcccgg
      841 tcggacctgt cggccaccca gagccgccgt cccccggacc tctacattgt caacactaac
      901 ccgatgggcc ggacgatcac tgccgactac aacactgttg tggtgtacta ccaggtgaca
      961 gccgacacca cggcgacctc gaccaaggcg gcgacgtctg ccacgtacca gaccacctac
     1021 gctgacatcc cgcagtcggt cgcccagcac ggccggctgg agtacttcct ggacgtgtcg
     1081 tcagccgggg tgatgaccca gccgcaggcg tcgcagatcg cggtcaacgt gctgaccaag
     1141 tacgtccggg ccaacttctc cggcaccttc accgtgcagc cggggcagct gctgaatgtc
     1201 gggggcttcc cagttgacct gggctgcaac tggggcggct acgtggcgtc ggtgcaggtg
     1261 gagaccttcg cctacggcgg cgaggtggcg ttcggcccgg tcactttcca gattggcgag
     1321 tacgccttcg atgacgacac ccagacggcg acagtgacgc cctaccagtc ggcccggacg
     1381 gacatcagct cagtcatcgc ccagctctac ccgggtaagt tttcatgaga tcttcctggg
     1441 tacttgtgct agccggcgct cgtgctttgg ggttcgtgat tacgatggcc ggcgcgaggg
     1501 gaatgggtgc gcgtatctgg cgtggccggc gaggacaaca tgggttgcgc cccaggactg
     1561 gccggcgcga catgactggg ttccgttagt accgtggccg ggcaaatacc tgagcatgat
     1621 gttctgggtg gtcttctggc cggcgagctg ccttgccctg ttcctgtgct acctcgggta
     1681 catcatgctc cggcggttct tctgactact gaggccactg gtggccgcag ccgcccatgc
     1741 ccttgtagct gaagtggtcg ccggggtgtt cctggtacag cttgcactcg cacttgcccc
     1801 tgcacttcgg gcagatcgcc ccgcagttgg gccggtcctc agacttcttt ttccgtccag
     1861 ccatgcttat cctcccttcg gtttccggta tccctgcggt cgctttcctt ccgggattat
     1921 gtcctggtca acggctgctg cccgcgactg gccccgggcc ggaacaggcc cccactccca
     1981 cgaattccgg gaatgccgtt catagggatc aggcatgcat tcctccttac tgccacgtgg
     2041 cagtcagcgc ctgtctttcc accgcatcca cagcagtgcg agaaacagga caatatcgaa
     2101 aaacagcaag gtcaggctgc tcacagcgcc ccgcaggtaa tgccgagggc gaccgcgaga
     2161 acagcaacaa cagcgaggta acggagtccg gggtcagcaa tcacccggtt accgtaccgg
     2221 aggcgagtgc ccacggcccg gcgtgcccgc cgtcggtggc gaccgcccgg accccgaccg
     2281 tccagttccc cggcgggacg ctgccgccct ggaagctgaa gtgcgaggag gagcccttgg
     2341 ggaagaaccg ggggtaggtc gccaggaccg ggccgcctgt cccctcgggg tagaccgtca
     2401 tctcgtagct gcccacaccg agcggcttgg gcggggtcgg tgagtagccg gaaacctgga
     2461 tgctggactt gccgaacttc gccgtcaggc tgcgcaccgg ggtgaagacc caggccgccc
     2521 cggagacgtc gtccagccac tgctcggaga aaatgtcggt gtcgtagccg agggcgtcac
     2581 tggaggcgaa ctgcaccccg atcaccggga acggccccga agcgttcatg acgtctgcga
     2641 tggcctgcac ctcgctcagg tcccagttgg cgacccacag gcccacgccg ctctgcacgc
     2701 cgccgctgac cagggtgttg accaccgggg tgatgttgct ggcgctggcg tagatggccg
     2761 ggtggcgctg gccgggccgc ctgatgccgt tccaggccgc ccgcgcttcc agcacccagg
     2821 agacgatgtg ccccagggtg gcggccccgc gctcgacgtc gaggatgtca ctggtggtga
     2881 tgctgccgct ggcgtcctgg tcgatgtgga gcgcgcccgg gtgcgccgcg aactgggccg
     2941 ccgaccagcg gatgtccggg gtgccggagt cgtagccggc gacctgcgac cccccgggca
     3001 gccgggagaa gttggccgag gtggcgtcaa aggcaagggt gaagctcact tgttcctccg
     3061 tggcggcggt gagtagcgga actcactggc ggatgcaggg actctgcgca gcatcatcgc
     3121 acgcagcttg ctgatcacat cttctctgct gtccccggca ctgagctgca cagtcacacc
     3181 cgggctggca acggggctgc actctacccg gggttcgtag taacagccac aggagtcctc
     3241 actgtagtca tcctggctgt aatgctgcca gccgtcagga aagttttccc agacgatcat
     3301 gtgcttgcag cttgcgcacc agtcatacgg cctgccgtcg gacaggtaac cagcgatcgt
     3361 cgtcttcgtc atcttcctca ctgactggct tcaggtgcgc tctgcgcagg tcttccgcgt
     3421 cttcgtactc gaacctgccg ccgtaggcca cgggggacgg gacaggctca gctgcgtccc
     3481 aggcgccgcc cttgcgccag acgatctcgt cgtcctcggg gaaggcgtac cagaacacgt
     3541 cggcccggtt tgcaaactcc tgccgggcga ccgggtcctc gcacaggaag ctgtagtgcc
     3601 cttgcatgtg cggtgctgtc ttgaccagga tctggcaact cactcgtcct ccaggtccgg
     3661 gtagagtccc cagctcttca tgaccttgcg gcaccagtgc ggcagcaggc acaagtccag
     3721 gggctgaccg tgcacgacgc agtactcgtg cggttctttt cccgggtcag gctcagccaa
     3781 cggtcctcct cgccagcggg tcggggcaca gttccgcctg ccgggcaccg tagatgcagt
     3841 gccgggcggc gtcgaaggcg tggttcatgt cgccgtggaa cgactccttg ccgaacacct
     3901 tctccagccg cctgtcactg gcccagggct tgacgtcggc tgccttgcgg atggtgacct
     3961 ggtacccgaa cagctgcagc tcctcggtca gctcgaccac cagctgccgg gtgaccgtcc
     4021 ccggcttgcc cgcgctgccg gacctcgcct ccgtccacgc ctcgaccgat gcgacccggc
     4081 gcatgaatcc tgccgggccg gtttcggtgt agtagttagc gagcactgcc cccagcacgt
     4141 gccgggccga ccgccagtcg acctgcagca gggtgtggcc caccacctgg ccgccggcgt
     4201 agtcgatgaa gcacaggccg gtgcgggggc cggggtccac cccgatggcg ctgtccacag
     4261 cggtcacagc cgggtcacgt cccgcagcgg cgctcccgtc ttcgggcaga cgcagtcagc
     4321 gccgatcgtg tccctgatcc tgagcagggt ccagcatttg ctgcagatcc aggcgatgtg
     4381 ccggaccgcg cagccgtcgc agtcccagct gatccggaag tcggcaggcc gggcgcagac
     4441 ggtggtcccg gtgatggtct cgcacgggtg cggggggcct gtcagcagtt cctccaggtc
     4501 ctccagggac tcgacggtca ttccgttcat gccatcctcc tcaggtaagc cttgcggttc
     4561 cacgggtgcc ggttgtccag catcgtgtgc caccagcacg tgccgttgtg gtggccgcac
     4621 cagtagtcgt agacccaggt ccggaaacag ggcaggccca gccggctccg gtggtgacag
     4681 cggttcctca cgccatctcc ttctcgatgt acgggcccca ctggtccgcc attgcccgcg
     4741 cgaagccctc gaatgtcagg ctccgcatgt actcccggtt gacccggccc cggctgtcct
     4801 cgtacttcga catcagcacc cgctgcgcgt tcctgtccgt ccgccacgag ccgccgccgg
     4861 tggccacccg cccgaccggg gcgaccgggt tgtccgccac cagcatcggc aggttccgca
     4921 gccacaggca ggtcttcttg ctgtaagggt ccccgaacca ccacggctcg accacctggt
     4981 cggggtagcg gtaccactgg ttcatcttgc ccatcgggtt ctcgaccgcg acgtgcgggg
     5041 ccggggcgta aatcatgtcc atgaacagct tggcgcccca ctgcatgcgc ccgtcgccgc
     5101 cctggctgat gtccttctcc ttccagtacc gcgcgcccgc cgagctgatg tcagtgcacg
     5161 gcgggaacgc gatgaccagg tcccacacag gttccagccc gaacttgacg cggctgttca
     5221 ccgggtggtc ccactcgaac agctccagca cgtcaccccg gtggtgccgg tagaccccgt
     5281 cgtgctcgaa gttctccgac acccagatgt gcatcagctg gtccgacggc tccaggtcgc
     5341 acgaccatgc ctcccagccg cgcttggcga actcggtggc gacccggccc gaccgctcgc
     5401 aggccgccag catccgcagc tgcctagctg gcacgggcca ccgccgcctg cagccactgc
     5461 ccggtcagga acagggcctg ggtctcggtg aaccccgctg tctccaggga caggaacaac
     5521 tcatgcgtga tgacggcgag ctgcccgacg ccgttcagcg ggtcctgcgg tccgctcata
     5581 ttttctccag actcgggggg ccgtactgct ctcccaccgt cacctcatag cctgccaggt
     5641 agacgatccc gtcggccgtc tcgctccagg tgaggttgct gtcagccgaa acctggcgca
     5701 ggtcgctcag ccaggccggg ctcaaggtca ctcgcgtaaa cccactcctc gttcgcggga
     5761 tcgaacgggg gatcgactct gctcacgcat gcctcctaaa ctgcttcctc ttccgggtag
     5821 gcgtcttcct cttgcgggta tgcgtcaccc gggtactcgt cgccggtttc ctcgaatgcc
     5881 cggcgggccc ggatgcgggc catctgctcg gcggtcatgg acgtgaagtg ccagaagccg
     5941 gtgtccgcgc aggtgtagta gtggacagta gccccggggt ggagcttgcc cactgtcgac
     6001 tcggcgtcgg cccgggtccg gtagcaccat ttgccgcacg ccgggcagcg gtgcatcccg
     6061 gcctggaccg cccgggggta gtccttgcgc cgccggaacg agtgctgcct gttcttgttc
     6121 cggcggcgct tactcgccac ggttacccgc acccatgtag ttctccttac tgccatgtgg
     6181 cattaagacg cgcccaggaa cgagcgggcg atccgggggt cctggaccag gtcggccagg
     6241 ttctccgcct tctcctggag ccgggcccgc acccgggact ccacgctgtt ctttgccacg
     6301 aaatcataaa tccgcaccgg cttgtcctgt cccttgcgcc acgcccggcg ctccgactga
     6361 acagcaggca cataccccca cggccgggcc aggaagacca cgtcccccga tgccgtcagg
     6421 ttgagaccga ccccgcccgc cccggtcgtc acgcacagca ggtccagctc gttgttctgg
     6481 aacgccaggc ggttccggtt gcggtcggca ggcttgacgt cgccgtcgat gaacgcgacc
     6541 tggtagccgg ccttcgccgc catcttgccc gccagccggg tcagctgggc gtactccgca
     6601 aacgcgatca ccggccgcga ccccaccgtg tgcccgtgac ggcggcccag cccgtcgaac
     6661 tccccctccg cctggtgaat ctcgtccagc aggtcaacca gggcctgccc cttccagcag
     6721 ggctcgcgca gggtgacctc ggtgtgcgtc acttcctcgc cgtacttcgg ggaccggggg
     6781 ttgccctcgg tttcccgggt gaccgccacg tcgcaggccg agcacgccag ctggcgcagc
     6841 cgcatcatct tgaccacggc aacctgggcc tccagcgggg tcatggagtc gggcagctca
     6901 gccagcatgt cctcctccat ctggttgtag gctgcgcgcc aggccggggg tatctcaatc
     6961 caccgggtct cgtacgtctt gggcggcatg tccaccacgt catcgagggc gacccggcgg
     7021 aaggtgccct gcatcgcagt gcggaactcg ggctcgcgga ccgggtcgag gccgatgatg
     7081 tcggcgtccc cgttgccgta ctgcggcccg ctggtgcgcc gcgacaggca gtagtggctc
     7141 ttgtagcggt cccgggcggg gtagctggcg gggtacatgc tgttcagcac cggccagaag
     7201 cccgctacgt tgcgggtgat cggggtgccg ctcccggcga cgacgttgcg gacgtgcttc
     7261 gccagccgcc gggcctcgcg ggactgcaaa gagttgtaat tacacaaagc gtggcactcg
     7321 tcaaagacaa ccgacccggc ccgcatccgc atcagcggcc ccggctttgc ctcgctgccg
     7381 gtgtcgttgc gcatcgtctc gtaccccatc accaggagct gggcgttgct gcggatgtac
     7441 ttgccgcgcc ccgtcccccg gtaagcgacc gccttccagt ccgggtacca ctgggggatc
     7501 tcctccagca gggtgtcgac caccccggcc ggggtcacga tcagggcggg ccacggctcg
     7561 cagttgcggg cttccagctc ggccaggccc atcaggtagg acccgctctt gccggtgccc
     7621 atctcgtcgg ccagcaggaa ccggccgttc atcccgatgg caacggcggc ggccgactgg
     7681 tgggccatcg gggtcagctg cggcggcttg ccggtgacgt cgccctggca ggaccggcgg
     7741 atgatctcga ccatgaccca ggcggacagc tccgcgcccg gcacccactt cagcccgggg
     7801 cggccgtccc agcctgccgc ccccgtaaac gcgcaggcaa gctgggtgac ggccgcccag
     7861 gacagcggca cctgcagcgc cgtctccgac accgggttga agttcggtgt catcgcgcgc
     7921 atgaccgcag cggcctggtt cagctcatga tcgcccgcgt ctgtcacctg gacgatgatg
     7981 aactgcccgc tggggtcaag ctccccgaag actgtcgcca tttacagctg cccgcctgac
     8041 cagagggtaa caaggatcat gcccgccacg accagcaggg caagcaggca gccgagggct
     8101 gcggtttgcg ccggacgggt cacggctact tctgcgttga gtggctggcg aacaggccgg
     8161 ccgatgtgaa gacctggtgc accaggttgc cggccgccgc cgggttctgg atgatccacc
     8221 agaccaccag ggcgccgatg atccaccaca gccagcgggg catcagtcct ccacctcctc
     8281 ttcgaccgtg accaggtggc ccttgccgtc cttcttgatg ccgatgacga ggttccgctt
     8341 ggccgctgcc ttcaggagca tgcgcttgac cttttcgcgt tctcgcgcgg gccctcgatc
     8401 cactgctggt cgatgacccg gtatctctta gccatgcgtg ccttgccttt ctcagggtag
     8461 ggaacgggca ggaggtactg ccgtgttcgg gattcttgcc ttcattctgt tcgtgatcgc
     8521 cgccatcctc gggtggaccg gcagcgaggc gcagcacgtg ctggcgatca tcgcggccgg
     8581 cctcgctgcc ctgacggcgg aggttgtctt cgcctggcgt ccctgggtcc acccgtaacg
     8641 ggaaccgtca gcgcaacggt cgccggcgcc tgccgccgca cccggcggta cggctgggtg
     8701 tccgcgttgc cccgggcctt agcctccgcc ttctcccggg tcagctgctg caccaggcgg
     8761 gccagctcgt cgcgctgcgg gttcacggcg ggggcatggt gtggtcaacg acggcgatgc
     8821 cgcagatgat gacggcagcg gcgccgatca gggcaaagat gatgacaacg gcccagtcgc
     8881 tgcgggtcac gaggcggtgc cgaacgtact gacgatcgtt ccgttgtaag tgcagccgga
     8941 cttgaagccg tggctggtct gccagttctg caggttgtcg agctggtcac tggtcagagg
     9001 gtggttgaac tgcacgtcct gctcctgccc cttggcgagg cggaccgtac tggtgcgggt
     9061 cagcgggctg aaaccctgct gcggccagct gatcttcagt ttgatgactt ccccgatgtt
     9121 gccggtgttg gtcacgtcga tgtcgcccgt agcgacagct gtgccgccgg ccgggttgtc
     9181 tcccagggtg taggcacaag caccttcata ggtaccgctg gtcgacgggg taaccgcctg
     9241 cggggtgtcg gccgggactg aggccgaagc tgccatcgag ggccgggtgg tgtccgcctg
     9301 gactgcgacg gtggagctgg acgaactctg gcacccggca acgagtgctg ccccggcggc
     9361 gatcaggacc ggggcgatga actttctcat gtggaactcc tgggtgtggt cttactgcca
     9421 cgtggcagtc agccgggcct ccctgacgcc cgaaaggaag gcccggcagc ttgtaactgt
     9481 ctagcgggtg cggaagctga tgctggcgct gtggccggcc ggcttgccgt tcaccgtggg
     9541 ctgcaccgtg acctggtagc ggctccacgg gcgcagcttg ccgaagtccg ccttgtcatt
     9601 atgcgtggcg aacgtccagc cgtggcggcc gagacccggg ccggtgacgg tgaccaggtc
     9661 ggtgctggtg accgtctggt tccagctgac ttcagccgag tgcgagctga cccagtcagt
     9721 gtgcaggtgc cggatcaccg gcttgggcgc ggggaccggg caggcgctga cgccggtaat
     9781 gtcacccgag gtggcctggc tgccgttgta agcgtcagtc caagtctggc agtgcctgga
     9841 gtcggtgtag acccaggtcc agttgctgat acccatgccg gtgaactggg tgccgggggc
     9901 gaagaagtac tccggccagg tgtccgtcgg cccggcgctg ccgctcagct tgaagggcac
     9961 gtccttcgca ctgaactggc tggtgttcgc ggtgaaggtg taggtgccgg acccggagac
    10021 cctggcgacc ggggcgccct tgatggcgac tgcctggctg ccgggcgagg tggccccggt
    10081 cagggcactg gcggtgccgg tgtcgtgcag ggtggcggtg tagtcgtaca ggccgctgac
    10141 ggtgtccggg cccaccaggc ggacagtgac agtgcggacc aggttgtcgt ccgcccaggt
    10201 gttgccgctg tagccggagt cggggcggtt ggtcaggtgc gtggtggccg tcgccgtgga
    10261 cggggttgcg ctcgcccagc cggtggtggc cagggtgcct gcgccagcgg ctgtgacaac
    10321 cgcgagcgag gcgaggattc gcttgagcat ccttactcct cattagggtt acttactgaa
    10381 cacatgttaa cacatgtgct cttctgatgc aaacggcccc tcctgctcgg tgggacagga
    10441 ggggccgtct tgcccagccg tcttcactcc ggctgggcag cttgcggggc tgctaggcag
    10501 ccggggtgat gacggttgag cccgcgtcca cgtcgaagtg gtcatcgaag aagacgtgcg
    10561 ggttcaggaa cagcaggccg tccttggtgc cgtcactggt cgcggcgacg ggcgagacga
    10621 gccaggcggt gctgttctgg aaggcaccct gcacctcgtg agtgccgttg acctcgctgt
    10681 tgccgttgac cttgacgtgt gcagcggtga acaggcggtt ggccgtgctc gcgtggtctg
    10741 ggtcaggaac cgcttcgttg agcgaggtag aggtggcgtt gtctgcgacc accccggccg
    10801 aggcgttgtt gaagttgagg cccgaccgca cacccggaat ggtgatctgg ttcgagtagc
    10861 tgtccgccat gtcgtccacg gcgtaagcag tcgcggtgcc ggccgagtgg tggcggccac
    10921 cgtaagagta ggcgtgcttc gggtcgtaca gcaggccgac ctcaaccgcg tgcgtcaccg
    10981 ggacattagc gagcaggacc actgcaccgg acagggtgtt gcccgtcgcc agcccattct
    11041 cacacttgtc gccgttgtac ctggcgtcac cgacgagcgc cgccgccgcg accacgtcca
    11101 tcttgccgcc gccgacgttc accaggccga cctgggcgac gtagccggcc gagttgcaaa
    11161 gctggatgcc agctccgccc tgaatcgcgc ccttctgact ggtcacgtta accgttgtgt
    11221 ccgattcaga agagcccagg cccgaaaggg agacagcagg caggacagca agaccgtggc
    11281 tgttgatacc gaggttggct tccaggtgcg tgaactggaa cagatcacca tgattcgagt
    11341 acgcctcgta acccgcaacc gagctggtgg cactggcctg ggtgtaccgc acgttgttgt
    11401 agttgacgcc cgcgtgagca acgccgctca gcgcgagggt cgcagcaccg gcaaggccgg
    11461 ccgccgcaaa gatggtcctc ttaaacaact cttctcctca ttcacagggt tctctgaggt
    11521 atgcgcgcga ggagcacgct accaggactg acccgggtgt gtcagtcgtg tcacctgatc
    11581 acacccgggt tgttacgtta ctgaccggga acctggatct ggtgaacctg cgccatgcgg
    11641 gcctgctcga cggtcagccc ggggatgaac cggacgtcgt cgggccagtc agctggctta
    11701 gaaggggata cactcgccgt tccagtcgca ccaggaccgg gctgggcaag cgggacagcc
    11761 ggggccggtg cgggagtagg gacgggctgc tgctgagccg ccgggttcca gtccgcgttc
    11821 ggctggccgt acaccggctg ctgcgggggc ggggactgct ggaactgcgg ctgctggggc
    11881 gggagccggc ctgccaccgt ctggtacggg ggctggcccg ggccgggcat ggcgaactgc
    11941 gtgccgccgg ggaccgggta gttgttcggg gacggctggc cccactgctg cggctgggca
    12001 ccctgctgcg gctgcgcctg gatctgctgc tgggcctgct ggacgtaggc cgggaccggg
    12061 gcttgtccgt ttcccttggt gtagacgacc ttcttcacct tggtgtcgct catgccgccc
    12121 cggttgggct tgctggcgat gtaggtgatg tcgatgacgt cgccagcccg gggcgggtac
    12181 atgaagccgg tctcggggtc ggtctccacc ccggcctcct gcatagcccg cagcagctcg
    12241 gcccgctcgt tgggcttgac gtaccaggtg gcctcgccgt tggggaactg cggcgacggc
    12301 tggaccacca gcggaatcca catggactcc ttggggcggc cgtcggggtg gcggtcgacc
    12361 gggtcctcac cgggccgggg ccgcatcttg gtcaccacgt ggacatcggc ggcggtgacg
    12421 ttgcggctga cggtgccgac gtagcgggtg cccagggtct cgaaggtgag gccggggccg
    12481 ccgccgcccg caggctggcc gaagaagccg gtcaggctgc cccggggaag ctcgggctcc
    12541 ggctgcatca tctgctgcgg ggtgccctgc tgctggggcg catagggctg cgtgtacgtc
    12601 gtctgggtgt actgggggta ctgcggctgc tggaactgcg cctgcggtac gggctgctgc
    12661 gggtaaccgg gggcgggctg gccgtaaggc tgctgcgacg ggtccgggta cggctgctgc
    12721 gggtaagtca ctggagtccc ttgtgtgcgg gtgggggacg gaagtcctgg acgtgggccg
    12781 tcccccgtgg tcaggtggcg tgaatccttg cgaaatgctg tgcgtgatgc gtgccgccgt
    12841 gtctggcttc agtgctcttt gctgtgcgtt atgcgttgtt tgtcatggcc ctggtcttct
    12901 tgcgccctgc cgagtacacc actgggtggg gatgcgcgac cgccgtgcgc cagacctcag
    12961 gatcggcctc tccggaccgc tttgcctggg ctcttcctgg caacaagctg aaccatagca
    13021 gcctgttagc tcactgtcta ctgttccgtg ttatgaatga caactgtcaa ctgtcctgca
    13081 ctcccgtcac cgacttcacc cgggtccatc cagcggtcgg acatcagctc ggtgaacggg
    13141 tactcgtccc agtccatgtc gccccggtag aacacccggg acgggtcggc cgccaggtag
    13201 aagacatcgc cgtccacagt ctcgcccagg gtcttgaggc agacgcgggc gcgcacctgg
    13261 ctcaggctgg gaacgtgctc gggcctggtc accatcacca gccccccgcc tcgatgcggg
    13321 ctgccgccct gcggagaacc ctcaccagca ccgtgcacca gaagctggcg ggccgggtgc
    13381 tgtactcccg cacgacctct gccgcgtcac gcagcacccg ggcgtcccgg ggacggtcac
    13441 gttccatcac ttctccacca gcgtccccgg gcagccgtac tgcccgtcat acctggcctg
    13501 cggacggtag agcccgcaga agtagcactc ggtgtcctcc ggcttggccg gcacgtccat
    13561 cagcgagatc ttcccggcct tcaccagctc cgcgacctgc tgccggaacg ccgtctgggc
    13621 cagcaccgac cggaccagct cgtcgtcggc cgccgtcggc acgtgcgacc agacgtacat
    13681 ctcgtccagg ctggacttgg tcctcggcca ggatacgatc accacccggt tcaccggcag
    13741 ccccaggttg aggtagccct ggcggtacag cagcagctgc acgtagtagt gcatgggcgg
    13801 cccctgggtc ttcagccgcg accgggtcga gtcgccctgg aacttgtggt cgaccacgca
    13861 ctggtagacg tggtcgtaca ggtcggccgt gcccgggtgc gggtcggggc cggggtcggg
    13921 cgtcacccgc cgctcggtca gccaccgggt gtagccgttg tgctcgttgt cccagtcgaa
    13981 cgccgccgcc acgaacgcat gcccggcggt gcccatcacc gaggaccaga cgtcggccac
    14041 gtggttggtc tgcttcgccc cggccatctt gccgaccacc tgccggtcgc actcgtggcc
    14101 cagctcggac ggcccgaggt gcacctggag ctggcgcggc agccgctggg cgtagcgggt
    14161 caggatgccc ttgatctcca ggctgtagcc ctcggcccag gcgtcgctgg cgttgaccga
    14221 ccggggagcg gccgacatgt aggaggaagc ggcgtcaggc acttaccgct tgctcttctt
    14281 cttgatgccg aaccgccaga agctcttctc ctgtttaaat gcttcgtaca ccgggggaag
    14341 gtgctcgcgg atcttcggcg ccggcaggaa gaactggctc tgccaggtgt aagtaaaccc
    14401 gggccacatc gggcccgccg ggatgtcgta gcccttgatg tccaggtccg ggtgcagcgc
    14461 gaccagctcc gcgaggatgc cgtccttcag ctcgtcgtag gcatcctggg cagcatcggc
    14521 cttggccttg gtgacctcgg ctatgcacag cagctgctcc aggcggctgt ccttcggcac
    14581 ctccacgatc acggtgcggt cccggacggg gacagtgatc tgggcagtat ccgtgtctgt
    14641 gtccatgtcc gggatcttat gccgggggtc cgacatttct cactgccatg tggcagtaag
    14701 gcgggcgtct aacaggtgag ttccgtggag gcagtggccc gcgcttgtgc agtgggctgc
    14761 gttcttggag tggcggggcg cccgttctct gggttccgta acttgcctgg ccccgcgcag
    14821 gtactttggg ctgcgtctgt gatgtggcgg ggcgttagga ggctgggttg cgttaacgac
    14881 atggcccctt acatggtgcg cacctggctt caggttacat acttagcctc atgaccgctg
    14941 ctgtcccgca cgcctaccgc tgcgaccgct gcaaggacac ccgggtgatc tgggacgggc
    15001 gccatctggt gcggtgcccg tcctgtaacc agggggtttc gtcctgcata ctggcacagg
    15061 gggaggtgcc cagtgccagc gggggatctg tttcagctgt tcggcggggc cggcgcgggg
    15121 gtcgtcgggg tctgcctgat ctttgtcatc ctgtttatca ccggccagat ctcgaccaag
    15181 aagcagcttg acgacgcaga gctgcgttac cagcagaaag ctgacgagtg cgaggaatgg
    15241 aaggaagcat ggaggctaga acgggcccga ggtgacgcca acgaggcaac cgggcggatc
    15301 gtcaaggacg tgatgctcgg gctgcgccag gtgggcaagg agctggagtt agatgtggcc
    15361 gttcaggcac aagaggaagc acatgactgt cgctgaagcc gggctggaag aagcgcggaa
    15421 ggcacgccgg tcggcagcgg agcgcctggc ggtcacaacg agtgaagtta ccatcccgtt
    15481 gcggaacctc cacaaggaga accacatcgg cccgcttctc gacgggctga tacagaagaa
    15541 agcagaacgg acgtacagaa cgtgacgcag gcccagctca tccacgacct tgacgagtac
    15601 gtcatctggt tctctttcct gctgtccgtg ctgttcgccc ccctggcgac catgatgtgg
    15661 ccgtggtgga agcacccctg ggggatcaac atcatatccc tcgaagtcgc catcgccttt
    15721 gccctgctgc cggcgtggct gtccatcgcc ttcggcctgc gccgggcgga tgtctacgtg
    15781 ttcgcctggg tccagctcgc ctccctggtc ctggtcgggc tggtcatcgc ctggcggctg
    15841 gtgctgatct ggaggaccca gcgccgggac accccgtccg ccgagcccgc cccgccgccc
    15901 cgggagcgcg acccggccag ctagtccccg tgcagggggc aggcgttctt gtcgttcggg
    15961 cacccgccga ccaggcagtg gccggcgatt cccttgagat gggggcagcg cttgcggctg
    16021 cagcggatgg tcagtctctg catgcgtcct cctcgtgggt gcgggcgtct gtccagtggg
    16081 ttccgttacc ggaatggccc gcatggggac cgccccggcc tgacccgggc gggggcggtc
    16141 gtggtgaggg gtgtcagcgg cgccagcagg ggggctgggg tgtaacgccg ccggggtcac
    16201 cctcgcgcgg ctgcaggcag gggcgcccgc tggccgcaac ccgtccctgg ccgtcgtaac
    16261 aggaacgggg tcctcgctcc cggcggctgt tcgcacaccc agggccagcc tgggcaaacc
    16321 gtgctcctac ccgccttccg tgtccccatg cctggctctg ccatatgccc caaagacacc
    16381 cgaagcaggc gggctgttgc aagcgcctga aaggtccaga ggcgccggca gccgccggga
    16441 agattcagtt ttcctgcaac agactggcac ggccccgccc cggtcgggtg cacacccagg
    16501 gaagttccgc cagagcggga ccgttgcctg tgatcttact tgttaacggg agtgtgtcaa
    16561 gtcacccttg agtaaatgac tgacgcaccg ccggggccgt cacttccccg ctggcttgca
    16621 cttgcaggag ctggccaccc ggctgcagcc ccggcagtac aggagaatcc gcgacggctt
    16681 cagcgggtcc ggtgcccggt acagggggcg cccgcaccgg cagtccccgt agccgctcac
    16741 cggaagagca cccacatgat gaacgcgatc accagcaggg caatgatgat cacgagggca
    16801 gtcggccagt tgacgctgcc gccgcccggc tgggctgccg cgattaccgc agtcatgctc
    16861 cttctccgtt ctggtaggcc cgcacccggg caatttccgt ttcgtcgcgg gagtacacgc
    16921 agcccgcgtc cttccggcag ggcaggaacc gcaggtgcgt gacgcacacc accgggccgt
    16981 cgtcgtccag gaagaacttg tcctgcgctg cccgcatgct tgcgtcagcc agctcgttca
    17041 gccagtttcc gtgcggcccc aggtctttcc gtcgcaccgg caggtccgcg tcggggtcag
    17101 cggcccacag caccgcgtcg agcacggcgt ccgggtgctt ctccgcgatc ttgtctatga
    17161 ccgccaccag tgcctcgctg tcgccttgca ggtcgttcag cagcagcgag atcttctccc
    17221 gggtccggcg gtggggcgtg cccttagccg ggtcgttgtc tgtccagcct ctgctcacgc
    17281 tggcctctct tcctctttgc cgcaggcgtc cggggtgccc tcgcacctgt agacgtcata
    17341 cggccggtta ctggtcagcg gtttcacccg ccgctgccac acccagttgt gctcatgctt
    17401 cttcggccgc tcgtgccgcc ctggtctccg gggcattcac ttgccgcagc cgtcgcggct
    17461 gatcccgcag tgctggccgt tgttgcagcc ggggcacgag caggtgcaga cgccgggctt
    17521 cctgttcatt ctgttctcct ttgctgtcag ccgcgctcct tgcaggcggc acagacgtgg
    17581 gcgccccggt gaccgggtgg cttgtcgcac acgtgcgggt tggaccggcc cttgccgctg
    17641 cgccggtcgc acagggacca gatatggccg cagagcttcg gcactcttga ctccttaatg
    17701 ccagctggca gtaacgttca tcttccccgg cttgattgcg gggcgctgac cagggaagct
    17761 ggtggtgtcc ccacccgcta ggggtgaaga atggatcacc gttccgacga ccgcattatg
    17821 gccgaactcc gcatcatgga gcgccggctg aacgacaagc tggaggaaat cttgaccgcc
    17881 cagaacgaca tcgacgctgc ggttacggcc ctcaccggcc tcgtctccga catccaggct
    17941 cagtccgcca acctcagcac tgccgtcacc gccatccaga atgagatctc ctcgctgcag
    18001 tcccaggggg tcgacacctc ggccctcgac gccgctgtgg cacaggtcgc ggacgcccag
    18061 tcggccctgg actcctcggt gtcctcggtc agctcggtcg tccccccggc gaacagctag
    18121 tcaccggctg ttcaggaacg cccccgcctg gctgtgatca gacgggggcg ttcgcttacg
    18181 gggcgtcctc gcaatgggta gcgacaggat tttggcccgc atgttgcggg gagtggattt
    18241 gaaccactga cctctgggtt atgagcccag cgagctaccg aactgctcca ccccgcactg
    18301 tgcccgccgg gactcccgga gtaggcgttc gcttgccccg gcggggtgct cacagcttac
    18361 ctgacggaac gttaggcgtc aaacgtttac cagtagtggt tcttcatgca gcggtgcctg
    18421 ccggtgtggg ggacgccctt ctggcaccac cacccgcacc ggggacaggg ctgcgtgcac
    18481 cttggcgtca ccgctccacc ccttccctgg catgcaccgg gcagccgttg cggctgttgc
    18541 tgcacatggt ccggctgcag tgcccggacg gccccttggg gtgcgagcag tcccactggg
    18601 tgcactgcgg ctcactcacc ggctcctcct ctaggccgcc gtcacaccct cc
//
LOCUS       DASZYN010000407         1665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1111826, whole genome shotgun sequence.
ACCESSION   DASZYN010000407 DASZYN010000000
VERSION     DASZYN010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111826"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VHE33_10515"
     CDS             <1..255
                     /locus_tag="VHE33_10515"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HVT97932.1"
                     /translation="PHCHQEIDIFSSGGAERTAKQFNIPFLGSVDLVPEIRQGGDTGM
                     PVALAGPESQHAAQFYTIAGKLAERAFAQAAKTENIFEIS"
     gene            complement(354..430)
                     /locus_tag="VHE33_10520"
     tRNA            complement(354..430)
                     /locus_tag="VHE33_10520"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(394..396),aa:Arg,seq:tct)
     gene            complement(408..977)
                     /locus_tag="VHE33_10525"
     CDS             complement(408..977)
                     /locus_tag="VHE33_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97933.1"
                     /translation="MEADWSVEIGQDAPVIDVDWPGFVDLQRDPGLIARIAESAGEPA
                     LRNALIAFNAAASPVFTSKCDMWELEAHELDPDEFGCGLQDACAAGSASWIDVVARDQ
                     QVFSSLQAHEDWVRKATDRLRREQIPCGRVDLVVRAAVAGGRGGFGITLYVAGCGVDT
                     QSAHKARDRVLGAAVAATMKDARASSSIG"
     gene            complement(979..>1665)
                     /locus_tag="VHE33_10530"
     CDS             complement(979..>1665)
                     /locus_tag="VHE33_10530"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVT97934.1"
                     /translation="LSPTIYHSGRSGDVGRVVETIIREQGLEAVALVGYSMGGNLVLR
                     WAGEVSGNPPKELKAVVGVSPLMDLAASSAALHRPQNRLYERHFLRSMLARVRRRIVM
                     YPRIYADAQVDRIHSMRDFDEYIVARYGGFADADDYYYSVASSKYAAKLRIPTLVVHS
                     DDDPFIRFLPATRTALIENPWVTFIETQHGGHCAFIAPATVPGDDGRWAEATLLRFLT
                     QGATAVSVGM"
BASE COUNT          325 a          527 c          515 g          298 t
ORIGIN      
        1 ccgcattgcc atcaggagat cgacattttc tccagcggcg gcgccgaacg cacggcgaag
       61 cagttcaaca ttccttttct cggatccgtc gatctggtcc ctgaaattcg gcagggcggg
      121 gataccggca tgcctgtcgc gttggctggt ccggaatctc agcacgccgc gcagttctat
      181 acgattgccg gcaagctggc ggaacgtgca ttcgcgcagg cggcgaaaac tgagaatatc
      241 ttcgaaataa gctagaggcg gttccggaga gttgtgccac ggctgactgg agtgagggtc
      301 tgaaccttcg caggttgccg atctgcaggc caggcacgcg cgcggggact taatggtggg
      361 cgaggcggga atcgaaccca caacccccgg cttagaaggc cggtgctcta tccgattgag
      421 ctactcgccc gcgcgtcttt cattgtagcc gccactgctg cgccgagaac ccgatcgcgg
      481 gctttgtgcg ctgactgcgt atccactccg caaccggcga catagagtgt gataccaaag
      541 ccgccgcggc ctcccgcgac tgctgcgcgc acgacaaggt ccacacggcc gcatggaatc
      601 tgctcacggc gcaagcgatc cgtcgccttc cgcacccagt cttcgtgcgc ctgcaaggat
      661 gagaacacct gctgatcgcg agcgacgacg tcgatccagc tggccgagcc cgccgcacat
      721 gcgtcctgca gtccgcatcc gaactcgtcc ggatcaagct cgtgggcctc gagctcccac
      781 atgtcgcact tgctcgtaaa gaccggcgag gcagcggcat tgaaggcgat cagagcattg
      841 cgcagggccg gttctccagc cgattcggcg attcgtgcga tcaggccggg atccctttgc
      901 agatcgacga agcctggcca gtccacatcg attacgggtg cgtcctgccc gatctcgacc
      961 gaccagtctg cctccacgct acatccccac gctgactgca gtcgcgccct gggtcagaaa
     1021 ccgcaacagc gtcgcttcgg cccagcggcc gtcatcgccg ggaactgttg cgggggcaat
     1081 gaaggcgcag tgcccgccgt gctgcgtctc gatgaacgtg acccatgggt tctcaatcag
     1141 cgccgtcctc gtcgcaggca ggaagcggat gaacggatcg tcgtctgagt ggacgaccag
     1201 cgtggggatg cgcagtttcg ctgcatattt cgaactggcg acgctgtagt agtaatcgtc
     1261 ggcatccgcg aaaccgccat agcgggcaac gatgtactcg tcgaagtcgc gcatggagtg
     1321 gatgcggtct acctgcgcgt cggcataaat ccgcggatac atgacgatcc tgcggcggac
     1381 gcgggccagc atcgaacgca gaaagtgccg ctcgtagagg cgattctgcg ggcgatgcag
     1441 cgcagcggag gaggctgcca ggtccatgag cggagaaacg ccgacgacgg ccttcaactc
     1501 ctttggcgga ttaccgctca cttccccggc ccagcgcagc acgagattgc cgcccatcga
     1561 gtagccgacg agcgcaacgg cctcaagtcc ttgttcccgg atgatcgttt ctacaacacg
     1621 accgacatcg cccgaacggc ccgaatgata gatggtcggc gagag
//
LOCUS       DASZYN010000408         1667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_981118, whole genome shotgun sequence.
ACCESSION   DASZYN010000408 DASZYN010000000
VERSION     DASZYN010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981118"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VHE33_10535"
     CDS             complement(<1..807)
                     /locus_tag="VHE33_10535"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HVT97935.1"
                     /translation="MKSALAALVVCSSIAVAQQGTTAAQAPNSDTSYIDANGAAHITR
                     VIPIPPDLSPEAKQSLSRQVSDAAKPQTLEERRHGTDVWQNRAGDVSEQIYPVHRSQG
                     TMAGVPVRIVTPVADMVPDRVLLNLHGGGFNSDSGSWTETIPIANLTKMKVVAVLYRL
                     APEHAFPAGIDDGIAVYKELLKTYKPEHIAIYGTSAGAIMTGEVAVKLRQLGLPLPGA
                     LGIFSGMGDFSREGDSRSMDALNGLSGHLDLLTASPHDPYYIASTDPRDPV"
     gene            complement(1101..>1667)
                     /locus_tag="VHE33_10540"
     CDS             complement(1101..>1667)
                     /locus_tag="VHE33_10540"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97936.1"
                     /translation="APTFPQPLKNQFSLLARWNSVAGAGHWPDADMLPIGYLGPKPGW
                     GQPRQTRLTRDEVRTMMTLWCIARSPLFIGTNLLKMDAFTESILTSPELIAVDQYGEN
                     NHPVIEKPDSVVWMASAPEQTGDYVAIFNLSDEPQTVNYPWRELGIQFGKFSVRDLWL
                     RKDLGRLDHIEATLPPHGSGLYMVQYQQ"
BASE COUNT          351 a          499 c          513 g          304 t
ORIGIN      
        1 caccggatcg cgcggatccg tcgatgcgat gtagtacgga tcgtgcgggc tcgccgtcag
       61 cagatcgaga tgtcccgaca gcccgttgag cgcgtccatc gaccgcgaat caccctcgcg
      121 cgaaaaatcg cccatcccgg aaaagatgcc cagagcgccg ggaagaggca gcccgagctg
      181 ccgcagcttc accgccacct cgcccgtcat gatcgctccg gccgacgttc cgtaaatggc
      241 gatgtgctcc ggcttatagg tcttcagcag ctctttgtag accgcaatgc catcgtcaat
      301 gccggcaggg aaggcgtgtt ccggcgccag ccgatacagc accgccacca ccttcatctt
      361 cgtgagattc gcgatcggaa tcgtctccgt ccacgaaccc gaatcggagt tgaagccgcc
      421 accatgcagg ttgagcagca cgcgatccgg caccatatcc gcgaccggcg tgacgatgcg
      481 caccggaacc ccggccattg tcccctgcga ccgatggacc ggatagattt gctccgagac
      541 atcgcccgcg cggttctgcc agacgtcggt gccatgccgc cgctcttcga gcgtttgcgg
      601 cttcgccgca tcggatacct ggcgcgacag cgattgcttc gcctcagggc tcagatcggg
      661 cgggatcgga atcacgcgcg tgatgtgcgc ggcgccgttc gcgtcgatat agctggtatc
      721 ggagttggga gcctgcgcgg ccgtcgtgcc ctgctgcgca acggcaatgg aagaacacac
      781 gaccagagcg gcgagagcag acttcatgca atttctcctc gatggaaaaa cgacgaggaa
      841 ggataacaga gccacaaaag cgaaaggccc gctttccagc gggcccgctt cagaggctga
      901 aggagggaat atcaggggaa atttgagggt cctgcgaaga agtctcgcag gcgattaaag
      961 gaagagactc atacaaccag gtaggttgga aacgtcagaa gtagcctctg gtgttgttga
     1021 tatagatgcg ctccgctgga gagagttgta tgcgtagagg aaaaatccgc atcaacaata
     1081 agtactgtat aatcaatgct ttactgttga tattggacca tgtagagccc cgatccgtga
     1141 gggggcaggg ttgcctcgat gtggtccagc cggcccaggt ccttgcgcag ccaaagatcg
     1201 cgcaccgaga acttcccgaa ctggatgccc agctcacgcc agggatagtt caccgtctgc
     1261 ggctcgtcgg agagattgaa gatggccacg taatcgccgg tctgctcggg ggccgacgcc
     1321 atccagacga ccgagtccgg tttctcgatc accggatgat tgttttcgcc gtactggtcc
     1381 acagcaatga gctcagggct ggtcaggatg gactcagtga aggcgtccat cttcagcaga
     1441 ttcgtcccga tgaacagcgg cgatcgcgcg atgcaccaca gggtcatcat ggtgcgcact
     1501 tcgtcgcgcg tgagccgcgt ctgccgcggc tggccccatc ccggcttcgg tccgagataa
     1561 ccgatcggca gcatgtcggc gtcgggccag tggcccgcgc cggcaacgga attccagcga
     1621 gcaaggagcg aaaactggtt cttcagcggc tgcggaaacg tcggcgc
//
LOCUS       DASZYN010000409         6783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_210162, whole genome shotgun sequence.
ACCESSION   DASZYN010000409 DASZYN010000000
VERSION     DASZYN010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210162"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="VHE33_10545"
     CDS             complement(<1..1125)
                     /locus_tag="VHE33_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HVT97937.1"
                     /translation="MFSAPSLRAAAVASCLAGAAFLLLHAATAPNPIRFELKRIPFTL
                     ENDETPAKNAPETMAGGVAVFDYNGDGRPDIYFANGANLSTLKKDDPKYRNRLFRNNG
                     DGTFTDVTDAAGVAGSGYDIGVAVADYDNDGCPDIFVGGVYRNTLYRNNCNGTFSDVT
                     ERAGIGNSKDPQYGPLWSEGGAWVDVNNDGLLDLFILDYMQWTYGPEPLCKMGDTGDY
                     CHPNNYKGQPNRLLLNNGDGTFRDVSKEWGIRDHVGKGMGVGVADYDLDGKQDLFVTN
                     DAEYNFLFHNLGRKFEEVAFDAGVTLPEGGQFISGMGLDFRDFNNDGYPDIAFVALNK
                     QTFPLFENTRKGDFREVTTPSGMRDLSLGMGGYGPALYDFD"
     gene            complement(1178..2272)
                     /locus_tag="VHE33_10550"
     CDS             complement(1178..2272)
                     /locus_tag="VHE33_10550"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HVT97938.1"
                     /translation="MTSQSDRSGASGADFSNRKWMLAGLAVVLMLCAGCQKSHDANTL
                     ATVNGKPILRADVETIFQNNLGDSHQTPSKVQAEIMRLNIVRQLIDEEIVMQRAAKQN
                     LVATDQEVSDRLTEIKAPFTQEEFQKRLDSQHMTLDDLKREIRRNRTEEKLFNKEITS
                     RINITDADIAAYYNEHKADFNLIEPNYHLAQIVVTAAPMPPQQVGNLQNSKAMNEAEA
                     KRKIEVLHNKLESGDDFAALAMNYSENPNTAGNGGDMGFVSDSQLKSNPEAYAAIAKL
                     KPGQISDILPIYGNSGSKQPVGYAVYKLIDREVAGQRQLNDPRVQQTIRQQLRDSREQ
                     LLKNAFLEILRNQGRVENYYAEEVFRNGSQ"
     gene            complement(2357..3343)
                     /locus_tag="VHE33_10555"
     CDS             complement(2357..3343)
                     /locus_tag="VHE33_10555"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HVT97939.1"
                     /translation="MLAAACSAQQTPNPAAPAAQTSPADPAPSNAAPVSAAPAAPSAL
                     DRKVEIMIRAELSVPPQYQIAIGPRQKSDVNGFDAIAVTFSLPNEPQRKQTVDFLLSK
                     DSNTLARLSKWDIGTDPVAMLPTEGRPVRGNPDAKVTIVNFDDLECPFCAAMNAEFFP
                     KTLEHYKDLVKFVYLDYPLVEIHPWAMHAAVNANCLAAQNPAAYWSYVDYLHTHGQDV
                     TGPDRDVAKSTAMLDKLASQEGARDHVDAAKLEACLAKQDDTKIMAEMRAADRAGVNA
                     TPTFFVSGERWAGQLDQRQLWIMIDRALTEQGITPPPSAAPATHAQAATPGK"
     gene            complement(3480..5996)
                     /gene="lptD"
                     /locus_tag="VHE33_10560"
     CDS             complement(3480..5996)
                     /gene="lptD"
                     /locus_tag="VHE33_10560"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HVT97940.1"
                     /translation="MRTRLFWCITLFLLLQQRPWAQTVTTQLPPPATPPDATQKAPAS
                     TADLPDDPSLQSQLPEAHVVPQPQAGTPVKLVYDNATRESQAKGNLYTLDGNVVIYYR
                     NYILHADHATYNDGTGEVEAQGHLMLDGGPDDEHFVADRGTINVNADTGDFFDVVGTL
                     GVERTPRGRMVFTAPNPFAVTGREVQQLGQGHYRVIHGTMTSCRLPKPDWRILSHEIE
                     LNNGVAKTSNGFFEFFHVPLMYLPYATHAVTEARVSGFSIPYVGNNTTKGFMIGEAVF
                     LTLGRSADMTVASQWWSKRGFAPSGIFRYRGRGANFGTLRVHSLLDKGVMEDNGQRGQ
                     NQGGVDVSGDGRYDLSPHTTAVVDSEYLSSYIYRLVFEEDYALAINSEVKSQLFVTHE
                     SNDMWTSLRFDRYQDFQNASVPGDEVRILHLPTIDFQTADRRLGGPMGLSPVWWGFSG
                     SAGAVSRFDYPNFRTTASVERVDIHPRVSMPLHFDGWTFRPEAAVRGTWYGKSQFPAN
                     LEQIPIVRKAGLPRTDVEAGFDLLPPVVERDFSAPWLERLLGGQIRHTIEPELGYRFV
                     TGINNFQQVLRFDDTDVASNTNELQYGVTQRLFLRRLHPHPCRGDEALGPDSMCGGGT
                     EDWITWRVAQKQYFDPNFDHAITPGTPNPLSTTLDFTGIDFLTDARHSTPVISRLRVR
                     TTGSTDVEWDVDYDAKEGKITSSNIFAGYRRGFYRFDFGDSYVNVPLGTTPLTTAKMP
                     PGPPTAPNSFNQIHVSAIYGAANKLGGSAGVSAAYDLVHQQLQYGALQTQYNWNCCGV
                     DFQYRRFSLGSIRDDTEYFYSVNFAGFASVGDLTHRISVF"
     gene            6333..>6783
                     /locus_tag="VHE33_10565"
     CDS             6333..>6783
                     /locus_tag="VHE33_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97941.1"
                     /translation="MASVNGSPTSSDAQTPLRETPVEPARAWKDEVAARVRAHRSRSP
                     RPTPVQQPSLPGLDEASASDAIAARVAERYARLPSWRETLAAQAAAKATVDSLVEAAS
                     VRPEEETETQPASVASAVPPSAPQEPAAEPWQPDLLRYSVSLDSLPAP"
BASE COUNT         1271 a         2197 c         1915 g         1400 t
ORIGIN      
        1 gtcgaagtcg tagagagccg gtccgtaacc gcccatcccc aggctcagat cgcgcatccc
       61 gctcggcgtg gtcacctcgc gaaaatctcc cttgcgcgtg ttttcgaaga gcggaaaggt
      121 ctgcttgttc agcgcaacaa acgcaatgtc cgggtagcca tcgttattga agtcccggaa
      181 atcaagaccc ataccggaaa tgaattgccc accttcgggc aacgtcactc ccgcatcgaa
      241 tgcaacttcc tcgaactttc ttcccaaatt gtggaacagg aagttgtact cggcgtcatt
      301 ggtcacgaac aaatcctgtt tgccatccag gtcgtaatcg gctacaccga cgcccattcc
      361 cttgcctaca tggtcgcgaa tgccccattc cttcgagaca tcccggaacg tgccgtcgcc
      421 gttgttcagc agcagccgat tcggctgtcc tttgtagttg ttgggatggc agtagtcgcc
      481 ggtgtcaccc attttgcaca gcggttcggg gccataggtc cactgcatgt aatccaggat
      541 gaacaagtcc agcagcccgt cgttgttcac gtccacccag gcaccgcctt cgctccacag
      601 cggtccatac tgcggatcct tcgagttccc gattcccgcc ctttcggtca catcagaaaa
      661 ggttccgttg caattgttgc ggtacagcgt gttccggtaa accccgccga caaaaatatc
      721 cgggcagccg tcattgtcgt aatcggcaac ggcaactccg atgtcataac cggatcccgc
      781 cacacccgcg gcatcggtca catcggtaaa agtcccgtcg ccgttgtttc tgaacaaccg
      841 attccggtac ttcggatcgt ccttcttcag ggtggagaga ttcgcaccat tcgcgaagta
      901 aatgtccggc cggccgtcgc cgttgtagtc gaagacagca acgccgcccg ccatggtttc
      961 tggtgcattc tttgccggcg tctcgtcgtt ctccagcgtg aacggaattc tcttcagctc
     1021 gaaccgaatc gggttcggcg cagttgccgc atggagcaga agaaaagcgg cgccggccag
     1081 acaactggcc actgcggcgg cgcgtaacga cggggcggaa aacatcttcc tgaagagtat
     1141 aggaaggcgc ggcgcgcgat tgcctgcacg gcccgcccta ttgagagccg ttccggaaga
     1201 cttcctcagc gtagtagttc tcgacgcgcc cctggttgcg cagtatctcc aggaacgcgt
     1261 tctttaacaa ctgctcgcgg ctgtcgcgca gctgttgccg gatggtctgc tgcacccgcg
     1321 gatcgttcag ctggcgctgc ccggccactt cccgatcgat cagtttgtag actgcgtagc
     1381 caaccggctg cttcgacccc gaatttccat agatggggag aatgtcgctg atctgccccg
     1441 gcttcagttt ggcgatggcc gcataagcct cggggttgga cttcaactgc gagtccgaca
     1501 caaatcccat gtcgccgcca ttccccgccg tgttcggatt ctccgagtaa ttcatcgcca
     1561 gcgccgcgaa gtcgtctccg ctttcgagct tgttgtgcag cacttcgatc ttccgcttcg
     1621 cctctgcctc attcatcgcc ttgctgtttt gcagattacc cacctgttgc ggaggcatgg
     1681 gcgccgcggt cacgacgatc tgcgccaaat ggtagttggg ctcgatcagg ttgaagtccg
     1741 ctttgtgctc gttgtaatag gcggcgatgt ccgcgtcggt gatgttgatc ctggacgtga
     1801 tttccttgtt gaacagcttc tcttcggtgc gattgcgccg gatttcccgc ttcagatcgt
     1861 ccagggtcat atgctgcgaa tccagacgct tctggaactc ctcctgggta aatggcgcct
     1921 tgatctcggt caggcggtcc gacacttcct ggtcggtggc caccaggttc tgtttcgccg
     1981 cccgctgcat cacgatctct tcatcgatca gctggcgcac gatgttgagg cgcatgatct
     2041 ccgcctgcac cttcgagggc gtctggtgcg agtcgcccag attgttttgg aagatcgtct
     2101 ccacgtcggc acgcaggatc ggcttgccgt tgactgtggc cagcgtattc gcgtcatgcg
     2161 acttctgaca gccggcgcac aacatcagca caaccgccag ccctgcaagc atccatttgc
     2221 ggttggaaaa gtcagcgcct gaagcgccgg aacgatccga ttgcgaggtc aaacagttct
     2281 ccctgctgcg gcgccgcaaa tcggggggcg gcgcgtactg ttcagaataa atgcggtccc
     2341 cgcaacctgc atagactcat ttccccggcg ttgccgcctg ggcgtgggta gccggcgccg
     2401 ctgacggggg tggagtaatc ccctgctcgg tcagcgcccg atcgatcatg atccagagct
     2461 gccgctggtc cagttgcccg gcccaccgtt cgccgctcac aaaaaaagtc ggcgtggcgt
     2521 tcacaccggc cctgtccgcg gcccgcatct ccgccatgat tttcgtgtcg tcctgcttcg
     2581 cgaggcaggc ttccagcttt gcggcatcca cgtgatcgcg cgcgccttcc tggctcgcca
     2641 gcttgtccag cattgcggtc gacttcgcca catcccggtc cggccccgtc acatcctgtc
     2701 catgcgtgtg gagataatcg acataactcc agtacgccgc cgggttctgc gccgccagac
     2761 agtttgcatt gaccgccgcg tgcatggccc acgggtggat ctccaccagc ggatagtcga
     2821 gataaacgaa cttcaccagg tccttgtagt gctcgagcgt tttgggaaag aactccgcat
     2881 tcatcgccgc gcagaagggg cattccagat cgtcgaagtt cacgatcgtt accttcgcat
     2941 ccggatttcc tcgcacgggg cggccttcag tcggcagcat cgctaccgga tccgttccga
     3001 tgtcccactt cgagagccgg gccagggtgt tcgagtcctt cgagagcaaa aaatcgacgg
     3061 tctgcttccg ctgtggctcg ttgggcaacg aaaacgtcac ggcaatcgcg tcaaatccat
     3121 tcacgtcgct cttctggcgc ggcccgatgg caatttgata ctgcggcggc acgctgagtt
     3181 ccgcgcggat catgatctcc accttgcggt cgagcgctga gggcgccgca ggagctgcgc
     3241 tcaccggagc cgcattcgat ggagccgggt cggcgggcga tgtctgtgct gcgggagccg
     3301 ccgggttggg tgtctgctga gcggaacagg cagccgcaag catgaagagc gcgcaggctg
     3361 cagagaaaga ccctctaaac aagaaaaacc accttcttcg gccgaacagc cgttacctca
     3421 gaatcgtctc tcccattatc gcagttcagg ctggggggcg gccccgttgg ctgctctcct
     3481 cagaacacgc tgatccggtg cgtcaggtct cccacgctgg caaaccccgc aaagttcacg
     3541 ctgtagaagt actccgtgtc atcgcgaatc gagcccagcg agaatcggcg gtactggaaa
     3601 tccaccccgc agcagttcca gttgtactgg gtttgtaacg caccatattg cagctgctgg
     3661 tggaccagat cgtacgctgc gctcacgccc gcgctccccc ccagcttgtt ggccgcgcca
     3721 taaatggcgg acacgtgaat ctggttgaag gaattaggag cagtcggcgg ccccggcggc
     3781 attttcgctg tcgtcagggg ggtcgtcccc agcggcacat tcacgtagga atcgccgaag
     3841 tcgaaccggt agaacccgcg ccgatagccg gcgaagatat tgctggaggt gattttcccc
     3901 tccttcgcgt cgtaatccac gtcccattcc acatcggtcg aacccgtggt tcggacccgc
     3961 aagcgcgaaa tcaccggcgt gctgtgcctt gcatcggtca gaaaatcgat gccggtgaaa
     4021 tccagggtcg tgctcagcgg attcggcgtc cccggcgtga tcgcgtgatc gaaattcggg
     4081 tcgaagtatt gcttctgcgc cacccgccag gtaatccagt cctcggtccc gccgccgcac
     4141 atggaatcgg gccccagcgc ttcatcgccg cggcaggggt gcggatgcag gcgccgcaaa
     4201 aacagcctct gcgtcacccc gtactgcagc tcattcgtgt tgctcgccac gtcggtgtcg
     4261 tcaaaacgca gcacctgctg aaagttgttg atccccgtta caaagcggta gcccaactcc
     4321 ggctcaatcg tgtgccggat ctggccgccc agcagacgtt caagccacgg cgccgaaaaa
     4381 tcccgttcca ccaccggcgg cagcaggtcg aacccggcct cgacatccgt tcgcggcagc
     4441 cctgccttgc gcacaatcgg gatctgttcc agattggcgg gaaactggct ttttccgtac
     4501 cacgttccgc gcaccgccgc ttccggccgg aacgtccacc catcgaagtg aagcggcatc
     4561 gacacgcgcg ggtggatgtc cacccgttcc acacttgccg tggttctgaa gttcggataa
     4621 tcaaagcgcg atacagcgcc cgccgatcca ctgaatcccc accacacggg ggaaagtccc
     4681 atggggccgc ccaggcgccg gtctgcggtc tggaagtcaa tggtgggcag gtggagaatt
     4741 ctgacctcat cgcccggcac gctggcgttc tggaaatcct gataccggtc gaaacgcagg
     4801 ctggtccaca tatcgttcga ctcgtgcgtc acgaacaact gcgacttcac ttcggagttg
     4861 atggcgagcg catagtcctc ctcgaacacc agccgataaa tgtaggagct cagatactcg
     4921 gagtccacta cggccgtcgt gtgcggcgaa agatcgtagc ggccatctcc actgacgtcg
     4981 actccgccct gattttgccc gcgctggccg ttgtcctcca ttacgccctt atccagcaga
     5041 ctgtgcacac gaagcgtccc gaaattcgcg ccccggccgc ggtagcggaa gattccgctg
     5101 ggggcaaacc ctcgcttcga ccaccattga gacgccaccg tcatgtcggc actgcgccct
     5161 aacgtcaaaa aaacggcttc gccgatcatg aagcctttcg tcgtgttgtt gccgacataa
     5221 ggaatggaga aaccggatac cctcgcctcg gtcaccgcat gcgtcgcata gggcaggtac
     5281 atcagcggca catggaagaa ctcgaagaac ccattgctgg ttttcgcgac gccgttgttc
     5341 agctcaatct cgtgcgagag aatccgccag tccggcttcg gcagccggca cgacgtcatg
     5401 gtgccgtgaa tcacccgata atgcccctgc cccaattgct gtacctcgcg accggtaacc
     5461 gcaaaaggat taggcgcggt aaacaccatc cgccctcgcg gggtccgctc caccccaagc
     5521 gtgcccacca catcgaaaaa gtccccggta tcggcgttga cgttgatcgt gccgcgatct
     5581 gcgacgaaat gctcatcgtc cgggcccccg tcgagcatca gatgcccctg cgcttccact
     5641 tccccggttc cgtcgttgta ggtcgcgtgg tcggcatgca ggatgtagtt gcggtagtag
     5701 atcaccacat tgccgtccag cgtatagaga ttgccttttg cctgcgactc gcgcgtcgcg
     5761 ttgtcataga ccagcttcac aggcgttccc gcctgcggct gcggcaccac gtgggcctcc
     5821 ggcagctggc tttgcaggct gggatcgtcc ggcagatcgg cggtgcttgc cggagccttt
     5881 tgcgttgcgt cgggtggtgt tgcgggagga ggaagctggg tggtaacggt ctgcgcccat
     5941 ggccgctgct gcaacaggag aaacagcgtg atacaccaaa aaagacgggt tctcatcgcg
     6001 cgcggcaact ccagcatagc aaagcagaat gccgaggttc ccacgcgtgt gacgcctcag
     6061 ccatcaacag gaaagcaatc ttcacggaat cgtggcttgc tgaacgcaca ttgtcatcgg
     6121 ccgcttgttg ggccgatgac ggaaaaaatt cactgcaaca ggattcatgg gaggactcac
     6181 gccgttcacc ccatgcaact cagccatcga ttgacgctcg ccgttcgcca gtctttgcga
     6241 aattgcttcg gccttgcccg cgtctgcatg aatccctgca caggcgttgt catggacgga
     6301 gtttcctctc aaaatgcgtg cagaatagga atgtggcatc cgtgaacggc tcccccacca
     6361 gttccgacgc gcaaaccccg ctccgcgaaa ctccggtcga accggcgcgc gcatggaaag
     6421 acgaagtcgc ggctcgcgtc cgcgcccacc gcagccgcag cccacgcccc actcccgttc
     6481 aacaacccag ccttcctggg ctggatgaag cctcggcctc cgatgccatc gctgcgcgcg
     6541 tcgccgagcg ttatgcccgg cttccgtcct ggcgcgagac gctcgccgct caggccgccg
     6601 ccaaagccac cgtagactcc ctggtcgaag ccgccagtgt tcggcccgaa gaggaaaccg
     6661 agacgcaacc cgcctccgtt gcttcggcag tccccccttc cgcaccccag gagcccgccg
     6721 ctgagccgtg gcagcccgac cttcttcgct actccgtcag cctcgattcc ctgcccgccc
     6781 ctc
//
LOCUS       DASZYN010000410         8345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1043390, whole genome shotgun sequence.
ACCESSION   DASZYN010000410 DASZYN010000000
VERSION     DASZYN010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8345
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043390"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..118
                     /locus_tag="VHE33_10570"
     tRNA            45..118
                     /locus_tag="VHE33_10570"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:77..79,aa:Gln,seq:ttg)
     gene            194..1255
                     /locus_tag="VHE33_10575"
     CDS             194..1255
                     /locus_tag="VHE33_10575"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HVT97942.1"
                     /translation="MKTDTIEAVGAATPNLAAAPGDGAKGKTQARRSRVSNEDRRFKV
                     FSGSANRELAEEICKHIGVTLGETRMQRFSDGEVYFQLLENVRGADVFVVQPTCWPVD
                     QHLVELLIMIDALKRASAGRITVVVPYYGYARQDRKDRPRVAISSKLVADLMTTAGAH
                     RALFMDLHAAQIQGFFNIPVDHLFASPVMVDYFRKLNLPDLVVVSPDAGGVERARHMA
                     TKLGVPMAIVDKRRTDINVTEVMHVIGDVTGRTCLIVDDIIDTAGTLVKTADTLLEKG
                     ATRVYAGASHGVLSGEAVERIAKSRLEQLVVTDSIPLRPEAQGLRDSEKIKVLSVAGL
                     LGAAILSIHMETSVSSLFS"
     gene            1411..2121
                     /locus_tag="VHE33_10580"
     CDS             1411..2121
                     /locus_tag="VHE33_10580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HVT97943.1"
                     /translation="MITQEIVNAVPREGKFNKNAARRVRAKGKVPAVVYGAAEPAVAV
                     EVDPKQIQKILHSETGHNSIFDLAVAGSTAKVMIVDWQYEPIKGKLIHIDFKRIALDK
                     PIRVEVPIQLTGVAIGVKTEGGILDQMLREVEIECLPGDIPNSIAVDISNLTFGTVLR
                     VADLPHSSKLKFLTSEDKPVAHVVSIKEEVVATPDAEAAAAAAATTPAEPEVVKKGKQ
                     ETADGEGAAAAGDKGGKK"
     gene            2122..2751
                     /gene="pth"
                     /locus_tag="VHE33_10585"
     CDS             2122..2751
                     /gene="pth"
                     /locus_tag="VHE33_10585"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HVT97944.1"
                     /translation="MGSGVFLVVGLGNPGIEYQFTPHNAGFLAIDRIASEHNVEVVNR
                     RCRALTARVQMAGREVILAKPETWMNLSGHSVGDLVDELDLELPGNLLVMYDELDLPL
                     GKLRIRERGSPAGHNGARSVAAALGTDEWWRIRIGVGPDAAVKEEAFRRGKDYLLTPM
                     GKRDLTAIDPVLDRVARAAEMVLTKGISGAMNEFNRRDPEESGNGAAEK"
     gene            2810..3277
                     /gene="rpsF"
                     /locus_tag="VHE33_10590"
     CDS             2810..3277
                     /gene="rpsF"
                     /locus_tag="VHE33_10590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HVT97945.1"
                     /translation="MMQRLYEVMFIVRPDVADEDVDKLIASLDQTVTGGGGTIRSTEK
                     LGKRKLAYLVRKFNDGNYVLLTIDADGSLIAELERRLRVSEPVIKFITVRMDEEEKRL
                     AKVRAIRATKKKVSAMPPPAAAVEPAPAEAAAEAVPAEAASAAAAETAAPASA"
     gene            3346..3669
                     /gene="rpsR"
                     /locus_tag="VHE33_10595"
     CDS             3346..3669
                     /gene="rpsR"
                     /locus_tag="VHE33_10595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HVT97946.1"
                     /translation="MADGTTTTTPSRPDAPAGRPSGPGGRGGPGGPGGRKFFRRKKVC
                     KFCTEKIDAISYRDVRLLQGFVAERGKIVPRRLTGVCTTHQRRLTRAIKQARNIALLP
                     FAARF"
     gene            3769..4227
                     /gene="rplI"
                     /locus_tag="VHE33_10600"
     CDS             3769..4227
                     /gene="rplI"
                     /locus_tag="VHE33_10600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HVT97947.1"
                     /translation="MEVILREDVSNLGHRGDVVKVADGYGRNFLLPKNLAMEATAANK
                     AVIEQMKSAAVRRSAKEKAEAEQLVTQLNNVALVFTRKVGEGDHLFGSVTSSDIAQEL
                     AGKGFNIDRRKVQLDEPLKSTGEFHVPVKLHREVTAHIAVTVKGEEAAAE"
     gene            4336..5175
                     /locus_tag="VHE33_10605"
     CDS             4336..5175
                     /locus_tag="VHE33_10605"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HVT97948.1"
                     /translation="MLKVISSHVFLNHRLHPGLLDVFARAGAQGVELFCARQHFDYTS
                     RTHVREVAEWFGANSAEAFSMHGPLWSDAEMGRSGAPTVNVIHPEKSRRIDAMDEIKR
                     ALEVAETLPFRFLILHLGEREDSWGPRALEHSITALEHLRAFANPLGVKLLVENLQGD
                     VARPSNLVEILTTGHFQDIGVCLDVGHAHLGDGVAASLSELNRYVRSSHLHDNKGDKD
                     AHLWPGDGTIHWEEALGGLKAAPQTPAGVLEIHYTLGEPVETVGEKARKAFEKMDAAE
                     KLG"
     gene            5184..6548
                     /gene="asnS"
                     /locus_tag="VHE33_10610"
     CDS             5184..6548
                     /gene="asnS"
                     /locus_tag="VHE33_10610"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR00457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HVT97949.1"
                     /translation="MADETAGTGTETATQASLSTIAGIGEHDGQRVTLHGWLYNLRES
                     GKLLFPIFRDGTGTIQGIVPKAAVTPEVFETVKGLTQESSVVVTGKVRADKRAPGGYE
                     LDVENVEVVQRVPAEDPFPISLKEHGTDFLMEHRHLWVRTPRQAAILRIRAEIIKAAR
                     DFLDDNGFVLTDPPILTPAACEGTSTLFPVEYFGDEAYLTQSGQLYIESTALALGKVY
                     SFGPTFRAEKSKTRRHLTEFWMVEPEWAFATLDDLMGLAENFISAIVQQVLTKRAAEL
                     KVINRDIAKLETIKPPFPRLRYDEAVKMLNDAHAKGLLEAPFEYGNDFGSPDETYISS
                     QFDRPVMVHRYPAAVKAFYMEPDPQDPKYALCVDVLAPEGYGEVIGGSQRIHSYELLK
                     KRIEEHQLPIDAFKWYLDLRRYGSVPHSGFGMGIERAVAWICGLDHVRETIPFARTLN
                     RIYP"
     gene            6555..7559
                     /gene="rocF"
                     /locus_tag="VHE33_10615"
     CDS             6555..7559
                     /gene="rocF"
                     /locus_tag="VHE33_10615"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HVT97950.1"
                     /translation="MPRSSETSLQAGSPASLPSPAGLYGGVQSLPPRKIKVIGVPLDL
                     GASRRGVDMGPSAVRVAGLEARLEALGHEVRDGGNISVAIAETKRSGHQSARYLKEIT
                     ETCTRVAEMVVKTLNEGMTPVVLGGDHSVAAGSVSGVAEYYRKQNQKIGLIWIDAHTD
                     INTPGTSPSGNVHGMPLAALVGLGPEPLANILGWAPKVQPENVVLIGVRDIDAAEKEN
                     VRRAGISEVYTMRDIDERGMRTVMEEALRAAGRGTAGYHVSLDMDWIDPEDAPGVGTP
                     VRGGATYREAHLAMEIIADHGRMVSLEVVEVNPVIDEHNRTADLAVELISSAFGKKIL
                     "
     gene            7670..>8345
                     /locus_tag="VHE33_10620"
     CDS             7670..>8345
                     /locus_tag="VHE33_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97951.1"
                     /translation="MRTKFLLLFFAVLGFLVMGYHPGIEDDGVYLSAVKSDLNPALYP
                     HNADFFRLEMKATCFDRWMADFVRVTHIPVAWSALLWQFTAIFLILVACWLIARQLFP
                     DEGARWGAVAMVSAMLTLPVSGTAIYIVDQHLHPRSLATALILTGVSCLLARKRWQAV
                     PLVLVAIALHPIMGILGACFCVVLEVALIQPATVMIPEADLAETAATLAVVPFGWIFA
                     RPTGIWL"
BASE COUNT         1670 a         2264 c         2872 g         1539 t
ORIGIN      
        1 ggctaacagc atttggctaa gtgcattttg ctgaacacga atactgggcg atcgactaat
       61 ggtaggtcaa gtgcctttgg agcactttgt ccaggttcga atcctggtcg cccagccaaa
      121 attttgtaac ggcccggcag attatcgccg cgatggaagc aggaaaactc aggcaaacca
      181 gctggaggat cttgtgaaga cggacacgat cgaagcagtg ggcgcggcta cgccgaatct
      241 ggcggcggct ccgggggacg gcgcaaaagg caagacgcag gcgcggcgtt cgcgagtatc
      301 gaacgaagac cggcggttca aggtcttttc cggttcagcg aaccgggaac tggccgagga
      361 gatttgcaaa cacatcggcg tcacgctggg cgagacgcgg atgcagcgct tcagcgatgg
      421 agaggtgtat ttccagctgc tggagaatgt gcgcggggcg gatgtttttg tggtgcagcc
      481 cacctgctgg ccggtggacc agcatctggt ggagctgctg atcatgatcg acgcgctgaa
      541 acgcgcgtcg gcaggaagga ttaccgtggt ggtgccgtat tacggatatg cgcggcagga
      601 ccgcaaggat cgccctcgtg tggcgatctc gtcgaagctg gtcgccgatc tgatgaccac
      661 cgcgggcgcg catcgcgcgc tgttcatgga cctgcacgcc gcgcagattc agggcttctt
      721 caacatcccg gtcgatcacc tctttgccag cccggtgatg gtcgattatt tccgcaagct
      781 gaatctgccc gacctcgtgg tggtttctcc cgatgcgggc ggcgtggagc gagcgcggca
      841 catggctacg aaactgggcg tgcctatggc gatcgtcgat aagcggcgta ccgatatcaa
      901 tgtcaccgag gtgatgcacg tgatcggcga tgtgaccggg cggacgtgcc tcatcgtcga
      961 cgacatcatc gacacggcgg gcactctggt aaagacggcg gacactctgc tggagaaagg
     1021 cgcgacgcgg gtgtatgcgg gcgcttcgca cggagtgctg tccggcgagg cggtcgagcg
     1081 catcgcgaaa tcgcgactgg agcagttagt tgtgaccgat tcgatcccgt tgcgccctga
     1141 ggcccagggg ctccgtgatt cggaaaaaat caaagtgctt tcggtggcgg gcctgctcgg
     1201 cgcggccatc ctgagcattc acatggagac cagcgtcagt tcgctgttca gctgagaagg
     1261 cagcttttcg ctactagctg ttagctacta gccagaagat tttgcacaac cataattggc
     1321 ggactgcagc gagtgcggtt cgtacagaag ggagcggaca acgcagctaa cacctaaaaa
     1381 gctgctgttc cgtgcccgaa ggaaaaggac atgattactc aggaaatcgt caacgcagtg
     1441 ccccgtgagg gcaagttcaa caagaatgca gcccgccgcg tgcgcgcgaa gggtaaggtg
     1501 cccgctgtgg tgtatggcgc ggcggagcct gccgtagcgg tggaagtgga tcccaagcag
     1561 atccagaaga ttctgcactc ggagacaggc cacaactcga tcttcgatct ggcagtagcc
     1621 ggttccacgg cgaaggtgat gattgtggac tggcagtacg agccgatcaa gggcaagctg
     1681 atccacatcg acttcaaacg cattgcgctg gacaagccga tccgcgtcga agtgccgatc
     1741 cagctgaccg gcgttgcgat tggggtgaag accgagggcg gcattctgga ccagatgctg
     1801 cgcgaggtgg agatcgagtg cctgccgggc gatattccga acagcattgc ggtggatatt
     1861 tcgaacctga cgtttggcac ggtgctgcgg gttgccgatc ttccccacag cagcaagctg
     1921 aagttcctga cttccgagga caagccggta gcccatgtgg tctcgatcaa ggaagaagta
     1981 gtagcaacgc cggatgccga ggcggctgca gcagctgcag caacgactcc tgcggagccg
     2041 gaagtggtga agaaaggcaa gcaggagacg gcggacggag aaggtgcggc agcggccggc
     2101 gacaaaggcg gcaagaagta agtgggaagc ggcgtgttcc tggtggtcgg gcttgggaac
     2161 ccgggcatcg agtaccagtt cacgccgcac aatgcggggt ttctggcgat tgaccgcatc
     2221 gccagtgagc acaacgtgga ggtggtgaac cgccgctgcc gcgcgctcac agccagggtg
     2281 cagatggcgg gccgggaggt aatcctggcg aaaccggaaa cctggatgaa cctgagcggg
     2341 cattccgtgg gggatctggt agacgaactg gatctggaac tcccgggaaa cctgctggtg
     2401 atgtacgacg agctggattt gccactgggg aagttacgaa ttcgggaacg tggcagcccg
     2461 gcgggacata acggagcgcg ttcggtcgca gcggcgctgg gaacggacga gtggtggcgg
     2521 atccggattg gagttggacc ggatgcggcg gtgaaagaag aggcctttcg gcgcggcaag
     2581 gattatctgc tgaccccgat gggcaagcga gatctgacgg cgatcgatcc ggttctggac
     2641 cgggtggcac gcgccgcgga gatggtgttg acgaagggaa tttcgggcgc catgaacgaa
     2701 ttcaaccgcc gggatcctga ggaatcggga aacggcgcgg cggagaagta aggaagacgg
     2761 aagcgaattt tggtttgccg ggcttgcggg cccggaagga agagaagcga tgatgcaacg
     2821 tctctatgag gtaatgttta tcgttcggcc cgacgtggcc gacgaggatg tggacaagct
     2881 gattgccagc ctggaccaga cggtgactgg cggcggaggc acgatccgct cgacggagaa
     2941 gctgggcaaa cgcaagctgg cgtatctggt gcggaagttc aacgacggca actacgtcct
     3001 gctcacaatc gatgccgatg ggtcgctgat cgcggaactg gagcgccgtc tgcgcgtctc
     3061 cgaaccggtg atcaagttca ttacggtgcg gatggacgag gaagaaaagc ggctggcgaa
     3121 ggtgcgggcg attcgcgcaa cgaagaagaa ggtgagcgcg atgcctccac cggcagcggc
     3181 agtggaacca gctccggcag aagcggcagc ggaagccgtt cccgcagaag cggcttcggc
     3241 tgcagcggcg gagacggcgg cacctgccag cgcgtaggtg cgctgggttc aacagagact
     3301 tgaaatttgg ccggcgccgc aagggcgccc gccggaggaa taaagatggc tgacggaacg
     3361 acgacaacaa caccctcaag gccggacgca ccggctggac gtcccagcgg accgggcgga
     3421 cgcggcggcc ccggcggacc gggcggacgc aaatttttcc gccgcaagaa ggtctgcaaa
     3481 ttctgcacgg agaagatcga cgcgatctcg tatcgcgacg tgcgtctgct gcagggattt
     3541 gtggccgagc gcggcaagat tgtgccgcgg cggctgaccg gcgtatgcac cacgcaccag
     3601 cggcggctga cccgggcgat caagcaggcg cgcaatattg ctctgttgcc gttcgcggcg
     3661 aggttctagg gcgcagcgag ccggcttcgc tgaccccttg ttagctgacc ggtgtatcgc
     3721 tgaccgcttt atcgctggcg gcctctggcc gccacaggag atctgaacat ggaagtgatc
     3781 cttagagaag atgtgtcgaa tctgggccac cgcggcgatg tggtgaaggt cgccgacggc
     3841 tacggccgga actttctgct gccgaagaat ctcgccatgg aggcgacggc ggcgaacaag
     3901 gcggttatcg agcagatgaa gtctgccgcc gtgcgtcgct ccgcgaagga gaaggcggag
     3961 gccgagcagc tggtgacaca gctcaacaac gtggcgctgg tctttacgcg caaggtcggc
     4021 gaaggcgatc acctgtttgg ctcggtgacg tcgtccgaca tcgcgcagga actggccggc
     4081 aaaggcttca atatcgaccg gcggaaggtt cagctcgatg agccgctgaa gtccaccgga
     4141 gagttccacg tgccggtgaa gctgcaccgc gaagtcacgg cgcacattgc cgtgacggtg
     4201 aagggcgaag aagccgcagc ggaataaatc tgagattcgg tcccggcggc cactgaagtc
     4261 tgccgtcggg gccgattccc tgccctgacc tcgcgggatg agaagatagg aagagctcat
     4321 tcctggtcct cattcatgct gaaggtcatc tcctcgcacg tattcctgaa ccatcggctg
     4381 catcctgggc tgctggacgt gttcgcccgc gcaggagcgc agggcgtcga gcttttttgc
     4441 gcgcgccagc acttcgacta caccagccgc actcatgttc gtgaagtggc ggagtggttc
     4501 ggggcaaatt ccgctgaggc gttctcgatg catggccctc tctggtcaga cgcagagatg
     4561 gggcgcagcg gcgcgcccac ggtcaacgtg attcatccgg agaagagccg ccggatcgat
     4621 gcgatggacg agatcaagcg agcgctcgag gtcgcagaga ccctgccctt ccggttcctg
     4681 attctgcatt taggggagcg tgaggattcg tggggaccgc gggcgctgga gcactccatt
     4741 acggcgctgg aacatctgcg ggcgttcgca aatcctctgg gcgtgaagct gctggtggag
     4801 aatttgcagg gcgatgtcgc tcgtccgtcg aatctggtgg agattctcac caccggtcac
     4861 tttcaggaca tcggcgtttg tctcgacgtt gggcacgcgc acctcggcga cggcgtggcc
     4921 gcgagcctct ctgaattgaa ccggtatgtt cgctcgtcgc acctgcacga taacaagggc
     4981 gacaaggacg cgcacctgtg gccgggggac ggaacgatcc actgggaaga ggcgctcggt
     5041 ggtttgaaag cggcgccgca gactcccgcg ggcgtgctgg agattcatta cacgctcggg
     5101 gagcctgtcg agaccgtcgg tgaaaaggcg aggaaggcct ttgaaaagat ggacgcggca
     5161 gaaaagctcg gttagaggcg gttatggcag acgaaacggc gggaactgga acagagacgg
     5221 cgacacaagc ttcgctgtcg acgatcgccg gcattggcga gcacgacgga cagcgggtca
     5281 cgctgcacgg gtggctttat aacctgcgcg agagcggcaa gctattgttc ccgatctttc
     5341 gcgacggcac gggaaccatc cagggcattg tgcccaaggc tgccgtaacg ccggaagttt
     5401 tcgagacggt gaaggggctg acgcaggagt cgtcggtcgt ggtcacgggc aaggtgcgcg
     5461 cggataaacg tgcgcccggc ggttacgaat tagacgtcga gaacgtcgaa gtcgtccagc
     5521 gtgtgccggc agaagacccg ttcccgatct cgctcaagga gcacggcacc gattttctga
     5581 tggagcatcg ccacctgtgg gtgcgcacgc cgcgacaggc ggcaatcctg cggattcgcg
     5641 ccgagatcat caaggcggcg cgcgattttc tggatgacaa cggatttgtg ctgaccgatc
     5701 cgccgattct gacgccggcg gcatgcgaag gcaccagcac gctgttcccc gttgagtatt
     5761 tcggtgacga ggcgtacctg acgcagagcg gacagctcta catcgagagc accgcactgg
     5821 cgctgggcaa ggtttattcg ttcgggccga cgtttcgcgc tgagaaatcg aaaacgcggc
     5881 ggcatctgac ggagttctgg atggtggagc cggagtgggc cttcgccacg ctcgacgacc
     5941 tgatgggcct ggcggagaac ttcatcagcg cgatcgtcca gcaggtgctg accaaacgcg
     6001 ctgcagaact gaaggtcatc aaccgagaca tcgcgaagct ggaaaccatc aagccgccgt
     6061 ttccgcgttt gcgttacgac gaagccgtga agatgctcaa cgatgcccac gcgaaaggtc
     6121 tgctggaagc gccgtttgag tatggcaacg attttggatc gccggatgag acatacatct
     6181 catcgcaatt cgaccggccg gtgatggtgc atcgttatcc tgcggcggtg aaggcgttct
     6241 atatggagcc ggacccgcag gatccgaagt acgcgctgtg cgtggatgtg ctggcgccgg
     6301 agggctacgg cgaggtgatc ggcggatcgc agcgcattca ctcctacgaa ctgctgaaga
     6361 agcggatcga ggagcaccag ttgccaatcg atgcgttcaa gtggtatctc gatctgcggc
     6421 ggtacggttc ggttccgcat tcgggctttg gcatgggcat cgagcgcgca gtggcatgga
     6481 tctgcgggct ggatcatgtg cgcgagacaa ttcccttcgc ccggacgctg aatcggatct
     6541 atccgtagtt ctttatgcca agatcgagtg aaacatcgct acaggccgga tcgccggcct
     6601 cacttccctc gcctgccggc ctgtatggcg gcgtgcagtc gctgcctcca cgcaaaatca
     6661 aagtgatcgg tgtgcctctg gatttgggcg cctcgcgacg cggcgtcgat atgggtccgt
     6721 ccgccgtgcg cgtggcgggg ctggaagcgc gcctcgaagc gctgggccat gaggtgcgcg
     6781 acggcggcaa catcagcgtc gcgatcgccg agacgaagcg gtcgggacac caaagcgcgc
     6841 gttatctgaa ggagatcacc gagacgtgca cgcgcgtggc ggagatggtg gtgaagacgc
     6901 tgaacgaggg catgacgcct gtggttctgg gcggcgatca ttccgtggct gccggcagcg
     6961 tgtcgggcgt ggcggagtac taccgcaagc agaaccagaa gatcggcctg atctggattg
     7021 acgcgcacac cgacatcaac acgccgggaa cctcgccgag cggcaacgtg catggcatgc
     7081 cgctggcggc gctggtgggg ctggggccgg agccgctggc gaacattctg ggatgggccc
     7141 cgaaggtgca gccggagaat gtggtgctga tcggcgtgcg cgatatcgat gcggcggaga
     7201 aggagaacgt gcggcgggcg ggcatcagcg aggtctacac gatgcgcgac atcgacgagc
     7261 gcggcatgcg cacggtgatg gaggaggctc tgcgcgcggc gggacgcggg acggcgggct
     7321 atcacgtttc tctggacatg gactggatcg accccgagga cgccccgggc gtgggaacgc
     7381 cggtgcgggg cggggcgacg tatcgggagg cgcatctggc gatggagatc atcgcggatc
     7441 acgggcggat ggtgtcgctc gaggtcgtcg aggtgaatcc ggtgatcgac gagcacaacc
     7501 ggacagcgga tctggcggtg gagctgattt cctcggcgtt cgggaagaaa atattgtgac
     7561 catgcagctt cctggagaga tttgacgtct gtccaaggga taggcaacgg cctggattca
     7621 tgaattctcc ataatcagga gaatatgatc gggggaggac acccctagtt tgcgcacaaa
     7681 attcctgttg ttgtttttcg cggtgctggg ttttctggtg atgggttatc accccggcat
     7741 cgaggacgac ggcgtttacc tgagtgcggt caagagcgac ctcaatccag ctctataccc
     7801 tcacaacgca gatttctttc gccttgaaat gaaggccacc tgcttcgaca ggtggatggc
     7861 agattttgtt cgggtgaccc acatccctgt cgcctggtcg gccttgctat ggcaattcac
     7921 cgccatcttc ctgattctgg tcgcctgttg gctcattgcc cgtcagctgt ttccggatga
     7981 aggagcgcgt tggggagccg tcgcgatggt ttccgcgatg ctgacgctcc cggtttcggg
     8041 aacggcgatc tacattgtcg accagcatct gcacccgcga tctctggcaa ccgctctgat
     8101 cctcactgga gtctcctgcc tgctggcgcg aaaaaggtgg caggccgtgc ctctggtgct
     8161 ggttgccatc gccctgcacc cgatcatggg catactgggc gcgtgctttt gcgttgtcct
     8221 tgaagtcgcg ctcattcagc cagccacggt gatgattccg gaagccgatc tcgccgaaac
     8281 agcggcgacc ctcgcggtgg ttccgtttgg gtggatcttc gcgcggccga ccggtatctg
     8341 gcttg
//
LOCUS       DASZYN010000411         9709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_20108, whole genome shotgun sequence.
ACCESSION   DASZYN010000411 DASZYN010000000
VERSION     DASZYN010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 9709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9709
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20108"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VHE33_10625"
     CDS             complement(<1..335)
                     /locus_tag="VHE33_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97952.1"
                     /translation="MLYLTAMTKGGSAGAGSALGGLWLGLCVLLLVLGARAQDTAYPP
                     QDAQIPGPAAKADAAAWRADVQRWRLERRMRMGYDGGKSTRPELGWTQRNFICAQMMI
                     EERDFYDPE"
     gene            340..415
                     /locus_tag="VHE33_10630"
     tRNA            340..415
                     /locus_tag="VHE33_10630"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:373..375,aa:Ile,seq:cat)
     gene            complement(474..3209)
                     /locus_tag="VHE33_10635"
     CDS             complement(474..3209)
                     /locus_tag="VHE33_10635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT97953.1"
                     /translation="MMAMIRRVAAMLRRRKLDGELDEELRAHLEMAAEENQRRGMAAD
                     EAWKAALRDFGGVTQVRETVREREGVLWVENMRRDLGYALRMLRKSPGFAAVAIGSLA
                     LGIGANTAIFSIAKHVLLDRLHVPHAQELRLFAWRSGKNSAVHSTWGDWNQTQDGVIC
                     TSFSYPVYEQMKKENRALSDLFAFKNVGRLNVTVNGEAEVVQGDLVSGNFYEQMEVQP
                     QLGRALGAGDDKVGASPVAVISDGYWARRFNRSASVIGKTILVNLVNVTIVGVNPAGF
                     TGAKGTMMSPEIIMPFSVQPVVFPYRNQASLLASDRVWWMQIMARAKPGVDEGRAQAA
                     LNVALEAAVRATMTVKKDESVPQLMVTDGSRGMNEAGRQMAQPLGFLMALVGLVVLLA
                     CANLANLLLSRSAARQREMSVRMARGAARGRILRQVMTESLLLAVMGGVAGLFVGWLG
                     RNAILAMLSSPAEPVAVQGGFDWGVFAFNAALSLLTGLLFGVAPAWQSTRAQVSTTLK
                     DTAQTVTRRRHGVAGKAIVGLQVALSTLLVFAAGIFLRSLVNLDRIDPGFDTKGLVLF
                     ELRPPQKEYTGEKQIQLFHQITDRLGTLPGVESVSLTSVAPLSNSTESDDFKPIGGKA
                     MDKDAAADNSTVGEQYFATFRVPILAGRGFAATDTETSPKVAVVNQALAREYFPNENP
                     IGKSFVTSGLRDEKITYQIVGICADTRYASLREEPPPVFYRNYRQSPEIDWGMTFAVR
                     TRAGRTAIAPELRRAVQSVDPNLPLTDVRTQEEQIDELLMNERIFADLTGGFGVLALM
                     LACIGIYGLMAYAVSRRTNEIGIRMALGARAEQVLGMVLREALGMTAAGIAVGLGAAL
                     ALSKLIASLLYGLKAWDPGTLAGAAALLALVALAASWVPARRAASVDPSRALRAE"
     gene            complement(3263..5887)
                     /locus_tag="VHE33_10640"
     CDS             complement(3263..5887)
                     /locus_tag="VHE33_10640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT97954.1"
                     /translation="MGLGIGRMWAMFRRRRLDGELDTELRAHLEMATEDHRRRGMSEI
                     EAREAALRDFGGVTQVRETVREREGLLWAENLRRDVLYAMRQMKKSPGFAAVVVLTLA
                     LGIGATTAMFTVVYSTLMRALPYPDAEKILALHDARIQGVSTGGLMGAPRFFDVKARN
                     RSFESLGFFFFDHPTLIAGRQLPVAVEAVGTNGEFWNVAGVRPLLGRVYGSQDDEPNA
                     PETAVLSYSGWQKIFGGDRSVIREQIRLDGRAVTIVGVMPAEFNMPSGVDLWHDAQFA
                     AADWKSYRGEGARFINVFGRLRPGVTLETAQADLDRIGEQLRAEYPHTDGLWRFTSQT
                     LRESRYGTMRPALLALVVAAALLLLIACINVANLLLSRATARQREVALRRALGASAGR
                     IAMQYLTESVVLGLAGGGAGVAAAFVLIRGVAAKLPGKLGVPGTVEMNWPVVLVALVI
                     ALGTGIAFGVAPVLESRRVELNTTMKQGEARVGGSGHKLRSALVSVQVGLSLILLVGA
                     ALMAKSLWHLLTNPLGFVPEHVLTVSVKVPWDTKDAGIRNFFGEVQRRLENLPGVQAV
                     GQMDALPTVDWHLRSNFDADWLPRMGNQPAINAEDRNVEGNFLGAMGVPLLAGRALTA
                     EDSREKNIPVMVNQELVQEYLPGGNPLGRHLLVNNTPHEIVGVIANLRGTAGAIASPP
                     GPEVYWPADGNGGVAGRYFVVRSQRDPEQLVSAIRKQVEAVDPQRAVGDVATMDELLN
                     HAVAQPRLHVTVVAALAGIALLLACVGIYGVVAYFVAQRTQEIGVRMALGATRGKIAA
                     LFVKRALVTAAIGLGGGTCASLALTQLLRSQLYGVQPNDPAMYGAAIAALLIPVAIAT
                     LRPALVAASVNPVEALRE"
     gene            complement(5974..6348)
                     /locus_tag="VHE33_10645"
     CDS             complement(5974..6348)
                     /locus_tag="VHE33_10645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVT97955.1"
                     /translation="MEDQDFLRPSAGEAGGQMLQGTLDLMVLKTLESMGPQHGYGIAR
                     RIEQISEQSLRLNQGTIYPALLRLRQRGWIRAEWGTSENGRKARYYALTKAGQAQIAR
                     ETESWERVAATVARFLKAANSQ"
     gene            6610..6807
                     /gene="rpsU"
                     /locus_tag="VHE33_10650"
     CDS             6610..6807
                     /gene="rpsU"
                     /locus_tag="VHE33_10650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HVT97956.1"
                     /translation="MAEVRIQEGEPLENALRRFKRKVQQEDIIKEVKRHSFYLKPGEK
                     RRVKEALARKRNRKKARKEQD"
     gene            complement(7747..8208)
                     /locus_tag="VHE33_10655"
     CDS             complement(7747..8208)
                     /locus_tag="VHE33_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT97957.1"
                     /translation="MKRRILLVDDDVAILLTLKAVLEISGFEVTTAASAREAKQKLKS
                     NEYHMVITDLRMESETAGEEVAAAAKKAANRPAVAMLTAYPPKDAEWVTTSDTDKVLV
                     KPMNTQDLLRQIEAMLVAHEDKKRVEAGKKVEPVVAEAATKKSKARPAVAK"
     gene            8328..>9709
                     /locus_tag="VHE33_10660"
     CDS             8328..>9709
                     /locus_tag="VHE33_10660"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HVT97958.1"
                     /translation="MAEYHVENFGCRASRADGEAIAANLRHGGLIPSQHLSTANVVIV
                     NTCSVTAEADRQARAWLRRVRRQNPQARVIVTGCYAQRAPEELAGLPQVDAVIGNSHK
                     ALVPEIALNFAAENNPVIPTDPSDNVATAFWRNSQFKRESNGPANGPAFVPLAAIVPK
                     VLHDDAFAHTELAALPFAPDARQTRPNLKVQDGCGNRCSFCIIPTTRGHSRSVPLATC
                     LDNVRRFVDNDGKELVLSGINLGRWGRDLQPAQRFEDLVAAILTRTSLPRLRLSSIEP
                     MDWSADLLALYREYAVTDPEADEVSNGRDSLNAGRRTPNAVLVNAERGTPNAVRHPAP
                     CGLHPRLARHTHLPLQSGSDTILRRMYRRYRPWHYAGKLAAIRALLPDAAIGADIMIG
                     FPGETDALFQESYDFIATQPFTYLHLFPFSARPGTPAWELHRQNPVPARAVQERMAAL
                     RELIAVKSRV"
BASE COUNT         1818 a         3745 c         2377 g         1769 t
ORIGIN      
        1 ttctccggat cgtagaagtc gcgctcttcg atcatcattt gcgcgcagat gaaatttcgt
       61 tgcgtccatc cgagttccgg ccgcgtggat ttcccgccgt cgtaacccat ccgcatccgc
      121 cgctccaggc gccagcgctg cacgtccgcg cgccacgccg cagcatcggc cttcgccgcg
      181 ggcccgggaa tctgtgcatc ctgcggcgga taagcggtgt cctgcgcgcg agccccaagg
      241 accaacagca acacgcacaa gcccagccac aggcccccaa gcgcggaacc ggcacccgcc
      301 gacccacctt ttgtcatggc agtaagatag agcacgaccg ggcctgtagc tcaacggtta
      361 gagcagggga ctcataatcc cttggttggg ggttcgaatc cctccgggcc caccacggtt
      421 caacgaccca gcggcgcagg tcatcccccc aaacccgact ttcaccagct tcctcactcc
      481 gcgcgcaaag cccggctcgg atccacagac gccgctcgcc gcgccggaac ccagctcgcc
      541 gcaagggcaa ccagagccag cagcgccgcc gccccggcca gcgtccccgg atcccacgcc
      601 ttcagaccat acagaaggct cgcgatcagc ttgctcagcg ccagcgcagc acccagcccc
      661 accgcgattc cggcagcagt catccccagc gcctcgcgca gcaccatccc cagcacctgc
      721 tccgctctcg cgcccagggc catccgaatt cctatttcat tcgtccgccg cgacacggca
      781 tacgccatca gcccatagat gccgatgcac gccagcatca gcgccaggac accaaatccg
      841 cccgtcagat ccgcgaaaat tctctcgttc atcagcagct cgtcgatctg ttcttcctgg
      901 gtccgcacgt ccgtcagcgg caggttcggg tccacacttt gcacggcgcg ccgcagctcc
      961 ggcgcaatcg ccgtccggcc tgcacgtgtt cgaaccgcaa acgtcatccc ccagtcaatc
     1021 tccggcgact gccggtaatt gcgatagaaa actggtggcg gctcctcgcg caggctcgca
     1081 taacgcgtat ccgcacaaat gcccacgatc tgatatgtaa tcttctcgtc cctcaggccg
     1141 gatgtaacaa agctcttccc gatcgggttc tcgttcggaa agtattcacg cgccagcgcc
     1201 tgattcacca ccgctacctt cggcgaagtt tccgtgtccg tcgccgcaaa accccgcccc
     1261 gccagaatcg gaacgcggaa cgtcgcgaaa tactgctcgc ctacagtgga attgtccgcg
     1321 gccgcatctt tatccatcgc cttcccgccg atcggcttga aatcgtcgct ctcggtgctg
     1381 ttcgagagcg gcgccacgct cgtcagcgac accgactcca cccccggcaa agttcccagt
     1441 cgatccgtga tctggtggaa cagctgaatc tgcttttctc ccgtgtactc cttctgcggc
     1501 ggccgcagtt cgaacagcac cagccccttc gtatcgaatc caggatcgat gcgatccaga
     1561 ttcacaagcg accgcagaaa aattcccgcc gcgaacacca gcagcgtcga caaggccacc
     1621 tgcaagccca caatcgcctt gcccgccact ccatgccgcc gccgcgtcac cgtctgtgcg
     1681 gtgtctttca gcgtcgtgct cacctgtgcc cgcgtcgact gccacgccgg cgccacccca
     1741 aacagcaaac ccgtcagcaa cgacagcgcc gcattgaacg caaacacgcc ccagtcgaaa
     1801 ccgccctgca ccgcaaccgg ctcggccggg ctcgacaaca tcgcaagaat cgcgttccgt
     1861 cccagccaac ccacaaagag tcccgcgacg ccgcccatca ccgccagcag caggctctcc
     1921 gtcatcacct gccgcagaat ccgcccgcgc gccgcgcccc gcgccatccg cacgctcatc
     1981 tcccgctgcc gcgccgccga tcgtgacagc agcaggttcg ccagattcgc gcacgccagc
     2041 agcaccacca aacccaccag tgccatcagg aaaccgagcg gctgcgccat ctgccgccca
     2101 gcctcgttca tgccccggct gccgtcggtc accatcaact gtggcaccga ctcgtccttc
     2161 ttcaccgtca tcgttgcgcg taccgccgcc tcaagagcga cgttgagcgc cgcctgcgcg
     2221 cgtccctcgt ccactcccgg cttcgcccgc gccatgatct gcatccacca cacccgatcg
     2281 ctcgcgagca gcgacgcctg gttgcgatac gggaacacta ccggctgcac gctgaacggc
     2341 ataatgattt cgggcgacat catggtcccc ttcgcgcccg tgaaccctgc cggattcact
     2401 ccgacaatcg ttacgttcac caggttcacc agaatcgtct tcccaatcac cgatgccgaa
     2461 cgattgaacc gccgcgccca gtacccatcg ctgatcaccg ccaccggaga cgcgcccacc
     2521 ttgtcgtctc ccgctcccag cgcacgcccc agctgcggct gcacctccat ttgctcgtag
     2581 aagttccctg aaaccagatc cccctgcacc acttcagctt cgccattcac cgttacgttc
     2641 agccgtccaa cgtttttgaa cgcgaacaga tcggagagcg cgcgattctc tttcttcatc
     2701 tgctcgtaga ccggatagga aaacgaagtg catatcaccc catcctgcgt ttgattccag
     2761 tcgccccagg tgctatgcac cgcgctgttc tttcccgaac gccacgcgaa caaccgcagc
     2821 tcctgcgcat ggggcacatg caaccgatcc agcagcacat gcttcgcaat cgagaagatc
     2881 gccgtgttcg ccccgatccc cagcgccagc gacccgatcg ccaccgccgc aaatccagga
     2941 ctcttccgca gcatgcgcaa cgcgtagccc agatcgcgcc gcatattttc gacccacaac
     3001 acgccctcac gctcccgcac cgtctctcgc acctgagtca cccctccaaa atcccgcagc
     3061 gccgctttcc acgcctcatc ggctgccatc ccgcgccgct gattctcctc cgccgccatc
     3121 tccagatgcg cgcgcagttc ctcatccagc tccccatcga gcttgcgcct ccgcaacatt
     3181 gccgccacgc gcctgatcat cgccatcatt cgagttccct ctacccgatc gccttttccc
     3241 tgctccctaa ttcctgccct tttcactccc gaagcgcctc aaccggattc accgaagccg
     3301 ctaccagcgc aggccgcagc gtcgcaatgg ccaccggaat caacagcgcc gcaatggccg
     3361 ccccatacat cgccggatca ttcggctgca cgccatacag ctggctgcgc aacaactgcg
     3421 tcaacgccag ggacgcgcag gttccccctc ccagtccaat cgccgccgtc accagcgccc
     3481 gcttcacaaa cagcgcggca atcttgcccc gcgtggcccc cagcgccatc cgcacgccaa
     3541 tctcctgcgt ccgttgcgcc acaaaatacg ccaccactcc gtagatcccc acgcacgcca
     3601 gcaacaacgc gattccagcc aacgctgcca ccacggtcac gtgtaaccgc ggctgcgcca
     3661 ccgcatggtt gagcagctca tccatggtcg ccacatctcc gacggcccgc tgcggatcca
     3721 cggcctccac ctgttttcga atcgcgctca ccagctgttc cggatcccgc tgcgaccgca
     3781 ccacgaaata acgtccggcc accccgccat tgccgtccgc gggccagtac acctccggcc
     3841 ccggcgggct cgcaatcgct cctgcggtcc cacgcagatt cgcaatcaca ccgacaatct
     3901 catgcggcgt gttgttcacc agcaagtgcc gccccagcgg attcccgccc ggcaggtact
     3961 cctgcaccag ttcctgattc accatcactg gaatgttctt ctctcgggag tcctccgcag
     4021 tcagtgcgcg cccggccagc aaaggcacgc ccatcgcacc caggaaattc ccctcgacat
     4081 ttctgtcttc cgcattgatg gccggctgat tccccatccg cggcagccag tccgcatcga
     4141 aattgctgcg caggtgccag tccaccgtcg gcagcgcatc catctgcccc accgcctgca
     4201 cgcccggcaa attctccagc cttctctgca cctcgccgaa aaagttccgg atgcccgcgt
     4261 ctttggtatc ccatggcacc ttcactgaaa ccgtcagcac gtgttccggc acaaatccca
     4321 gcggattcgt caacagatgc cacagcgact tcgccatcag cgctgcaccc accagcagaa
     4381 tcagcgacag ccccacctgg accgacacca gcgcgctgcg caatttgtgc cccgatcctc
     4441 ccacccgcgc ctcgccctgc ttcatcgtcg tgttcagttc cacgcgccgg ctctccagca
     4501 cgggagcgac cccgaacgcg atcccggtcc ccagcgcgat cacaagcgcc accagcacca
     4561 ccggccagtt catctccacc gttcctggaa cgccgagctt tcccggcagc ttcgccgcca
     4621 caccgcgaat cagcacaaag gccgcagcta ctccggcgcc gccgcctgcc agacccagta
     4681 cgacactctc ggtcagatac tgcatcgcaa tccgccctgc cgaagcgccc agcgcccgcc
     4741 gcagcgccac ctcccgctgc cgcgccgtcg cgcgcgacaa cagcaggttg gccacattga
     4801 tgcacgcaat cagcagcagc agcgccgccg ccaccaccag tgccagcagc gccggccgca
     4861 tcgtcccata gcggctctcg cgcaaagtct ggctcgtgaa cctccacagc ccgtcggtgt
     4921 gcggatactc cgcgcgcaac tgttccccga tgcgatccag atcggcctgc gccgtctcca
     4981 gcgtcactcc agggcgcagc cgcccgaaca cattgatgaa ccgcgctccc tcgccccgat
     5041 aactcttcca gtccgctgcg gcaaattgcg cgtcatgcca aagatccact cccgacggca
     5101 tgttgaactc ggccggcatc accccaacaa tcgtgaccgc cctcccatcc agccggatct
     5161 gctcccgaat cacactccga tcgccgccga aaatcttctg ccatccgctg tagctcagca
     5221 ccgccgtctc cggcgcattc ggctcatcgt cctgcgaacc atacactcgt cccagcagtg
     5281 gacgtacccc cgccacgttc cagaactccc cgttcgtccc gactgcctcc accgccaccg
     5341 gcaattggcg gccggcaatc agtgtcgggt gatcgaagaa gaaaaagcca aggctctcga
     5401 acgaccgatt ccgcgccttc acatcgaaga accgcggcgc tcccattagc cctccggtcg
     5461 acactccctg aatccgcgcg tcatgcaggg ccaatatctt ttccgcgtcg ggataaggca
     5521 gggcccgcat cagcgtcgag tacaccaccg tgaacatcgc cgtcgtcgca ccaattccca
     5581 gcgccagcgt cagcaccacc accgccgcaa accccggcga cttcttcatc tgtcgcatcg
     5641 catagagaac atcgcgccgc agattctccg cccacaacaa accctcgcgc tcccgcaccg
     5701 tctctcgtac ctgcgtcacg cccccaaaat ccctgagcgc cgcctcccgt gcctcaatct
     5761 cactcattcc ccggcgccga tgatcctcgg tcgccatctc cagatgcgcg cgcagctcag
     5821 tgtccagctc cccgtccaac ctgcgccgcc gaaacatcgc ccacattcgg ccgattccca
     5881 ggcccatccg acgccccttt cccaggtacc cgcttgttgc ccgtttcccg ttgccgctgc
     5941 ctttcctatt gactattggc tattggctac tggctactgg ctattcgccg ccttcaaaaa
     6001 ccgggccacc gtcgcagcca ctcgctccca gctctcggtt tctcttgcaa tctgcgcctg
     6061 ccccgccttc gtcaacgcgt aataccgcgc cttgcgcccg ttctcgctcg tcccccactc
     6121 cgcccgaatc caaccccgct gccgcaatcg caacagcgcc ggataaatcg tcccctgatt
     6181 gagcctcagt gactgttcgc tgatctgctc gatccgccgc gcgatgccgt atccgtgctg
     6241 cggccccatc gactccagcg tcttcagcac catcaggtcc agcgtgccct gcagcatctg
     6301 cccgcccgcc tcgccggcgc tggggcggag aaagtcttga tcctccacat ctcctcctgt
     6361 tggagttcta cagatacgcc atcgcccagt agattgtcaa caggaagcca gatttctggc
     6421 gggataggat tcagcatgcg aaggagaaga gcctgactca gcgccattcg aattccgtat
     6481 ctttttcaac cgtttgacgc tttttgacgt taccggctgc cgcacactgc ccggccttta
     6541 tggaaaatta acgtcgagtt cctgtaatct gatcgttgcc caccatgggt cgtctggagg
     6601 tgtggttctt tggcagaggt tcgcattcag gaaggcgagc cgctcgaaaa tgcattgcgc
     6661 cggtttaaac gcaaggttca gcaggaagac atcatcaagg aagtcaaacg tcattccttc
     6721 tacctgaagc ccggcgaaaa gcgccgcgtc aaagaggcgc tggcacgaaa gcggaatcgt
     6781 aagaaagctc gcaaagagca ggactaatca ctcacgccgg tctgtccagg tccccagact
     6841 ccgacagatc tgagcttcgc cggccaccat cgccggcgaa gtggagacca ggccctcatg
     6901 agcgccccgc tcgcgccgtt gggttggcag accggtaagt atctcttctg atctgactcc
     6961 ggtccctcag acggagggtt ttcggcgctt tcgccggtgt actctttcgc cgcagttgca
     7021 ggcggccgac agttgcgaga tagatccgca ccgagatgtc gaacccggtc cggaccgctg
     7081 cactcgctgc tgccaaacgg gcccgatcca attcagaagt accgcaccgg cctcataccg
     7141 gcgtgtcgca gcaagccggc gtctgctttt ggcagccgct gggcgacccg gacgcgccag
     7201 ccgtttggcg tgattccgcg ggtgcttcgg gcaaaggcga gggaagcggg atggatttcg
     7261 tcgacaaggt gctgaagtgc gcggactgtg gaaccgactt cgttttcacg gccggcgaac
     7321 aactgttctt ttacgacaag cagttcaaga acgatccgaa acgttgcaag caatgcaagg
     7381 ccaaacgggc ttccggctcc ggctccgcgc gtccggaaac gcgcaccatc tgttcagaat
     7441 gcggaacgga aaccaccgtt cccttcaagc ccactcaggg acgaccggtc ctctgccgta
     7501 gctgtttcca gcagaaaagg accatgagtc aggccagcta gcctctcgaa ccgccttcgc
     7561 gggtcgctga tgcaccgttc ccgaacgctc tcgcgcgagt tgtgtgcctc aaggccataa
     7621 tctgtcttcc tgtgcgggag ccgtccacac tgtcccaccc ggtcaccgcc gttttcgctc
     7681 cagacgcgaa acaactcgcc ggaaagatcc ggcgagttgc agatgactga acgctcgatc
     7741 cccggtctac ttcgcgacag cgggccttgc cttcgatttc ttcgtcgccg cctcagcaac
     7801 caccggctcg accttcttcc ccgcttccac ccgcttcttg tcttcgtgcg ccaccagcat
     7861 cgcctcgatc tgccgcagca gatcctgcgt gttcatcggc ttcaccagca ccttatccgt
     7921 atccgacgtc gtcacccact cggcatcctt cggcggatac gccgtcagca tcgctaccgc
     7981 cggccggttc gccgccttct tcgccgctgc cgcgacttct tcccctgccg tctcgctctc
     8041 catgcgcaga tcggtgatca ccatgtggta ctcgttcgac ttcagcttct gcttcgcttc
     8101 tcgggccgac gccgccgtcg tgacttcaaa tccgctgatc tcgagcaccg cctttaaagt
     8161 cagaagaatc gcaacgtcgt catcgactaa aagaatgcgt cttttcataa gaactcctgg
     8221 gtcccaccgc tggatcgtac acgaaaaagc ctgacaaacg cttgagagaa gggattccct
     8281 gcggcctctg ggccgggcca gcctcccccg ttgatacact gagcaacgtg gcagaatatc
     8341 acgtcgaaaa ctttgggtgc cgcgccagcc gcgccgatgg cgaggccatt gccgccaatc
     8401 tgcgccatgg cggccttatt ccttcccaac acctttcgac ggcaaatgtt gtcatcgtaa
     8461 acacctgtag cgtcaccgcg gaagccgatc gccaggcccg cgcctggctc cgccgcgttc
     8521 gccgccagaa cccccaggcg cgcgtcatcg tcactggctg ttacgcccag cgcgccccgg
     8581 aggaactcgc cggactcccc caagtcgacg ccgtcatcgg caacagccat aaagccctcg
     8641 ttcccgaaat agcgctcaat ttcgccgctg agaataaccc agtcatcccg accgaccctt
     8701 cggacaacgt ggcgacggct ttttggcgca acagtcaatt caaacgggag tcgaatgggc
     8761 ctgcgaatgg gcctgctttt gttccgctcg ccgcgatcgt cccgaaggtc ctccacgacg
     8821 atgccttcgc gcacaccgaa ctggccgcgc ttcccttcgc gcccgacgcg cgccagaccc
     8881 gccccaacct caaagtccag gacggatgcg gcaatcgctg ctccttctgc atcatcccca
     8941 ccacccgcgg ccattcgcgc tccgtccccc tcgccacctg cctcgacaac gttcgccgct
     9001 tcgtcgacaa cgatggcaaa gagctggtct tgtccggcat caacctcggc cgctggggcc
     9061 gtgacctcca acccgctcag cgcttcgaag acctggtcgc cgccattctg acccggactt
     9121 ctctcccccg cctccgcctc agttccatcg agccaatgga ctggtccgct gacttactcg
     9181 ccctctaccg cgaatacgca gtgaccgatc ctgaagccga tgaagtttca aacggacgcg
     9241 attcgctgaa cgctggacgc cgaacgccga acgctgtcct ggtgaacgcc gaacgcggaa
     9301 cgccgaacgc cgttcggcac cctgcaccct gtggcctgca ccctcgtttg gcgcggcaca
     9361 cccatctccc gcttcagtcc ggctccgaca ccatcctccg ccgcatgtat cgccgctatc
     9421 gtccctggca ctatgccgga aagctcgctg ccattcgcgc actcctcccg gacgccgcca
     9481 tcggcgccga catcatgatc ggattccctg gcgaaaccga cgccctcttt caggaaagct
     9541 acgactttat cgccacgcag cccttcacct atctccacct ctttcccttc tccgcccgcc
     9601 cgggcacacc agcctgggag ctccaccgcc agaaccccgt ccccgcgcgc gccgtgcagg
     9661 agcgcatggc cgctctgcgt gaactcatcg ccgtgaagtc ccgcgtctt
//
LOCUS       DASZYN010000412         7772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_725868, whole genome shotgun sequence.
ACCESSION   DASZYN010000412 DASZYN010000000
VERSION     DASZYN010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725868"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..1444)
                     /locus_tag="VHE33_10665"
     CDS             complement(302..1444)
                     /locus_tag="VHE33_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HVT97959.1"
                     /translation="MRIRPRCFAALVVSLFLPLLASAQNPRTSTAFERAAHLQHGINT
                     SMWFAQASDYSPQRLRSFTTADDIALIAKMGFDHCRLSIDGDQLMTWFYTQRDVHHST
                     GFMEALDRVVKTMLDNHLSVIIDVHPTSQFKAELFQGAGGIGNFVELWRALAGHYAAA
                     DPEHVFFEIMNESEQQDPWRWHGIEAQVVQAIRQVAPDNTIIASGAHWSGLPDLLATE
                     TLADDNVIYTFHDYEPFPFTHQGATWTNPEVRPERAIPYPSTPENIAPKLNQEPDLAG
                     QYFLDEYGENRWDAARVENSIEFAAKWSALHHAPVYCGEFGVFREFAPPAARAQWLHD
                     MRTAMEKNHIGWAMWDYQGGFSVVTKENGVTTPDRAILNALGLHMP"
     gene            1507..2337
                     /locus_tag="VHE33_10670"
     CDS             1507..2337
                     /locus_tag="VHE33_10670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HVT97960.1"
                     /translation="MTNRSHKPDPNAAHNPGSRPAGAVDEATSAALVREMFDRIAPRY
                     DLLNHVLSCNVDRTWWWRTARTFRHILERPDAKVLDICCGTGDMTRALLRRRPGGNEK
                     QPAEPVIAADFSEKMLALARVKLAGRQVSIQSVDAMRMPFADGSLDLITTAFGFRNLP
                     NYKAGLEEFLRVLKPGGEVGILDCSRPGGVLGKGFGFYFRHVLPAIGARISGDAVAYG
                     YLPSSVEGFPPPPAMVELMRSVGFVDVQWTPYTFGIAGLWRGRKPPGFQSSPLSVQVG
                     "
     gene            2345..3781
                     /locus_tag="VHE33_10675"
     CDS             2345..3781
                     /locus_tag="VHE33_10675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HVT97961.1"
                     /translation="MMSSAALTKQTHARAEKRWVSLVSVMAACAMTVLKAAVGIMTGS
                     LGVLSDAAHSGLDLVGALMTFLSVRVSDKPADWNHPYGHAKIENISAFIETGLMAASA
                     LWFSGEAISRMFFHPVSLRYSWWPFAVLVISMGVDLWRSAQLRRVAKQHQSAALEADA
                     LHFASDIWTSVAVVLGLAASWVGVVHHVGWLRFADPAAALVVSAMILVFGWRLAWRAV
                     AALTDQISPELHTKMIDAVRETDGVLGIDQARVRKSGNHYFADLTLSLPRQLTFQSTE
                     ELVERATGAVQRVLPGADVVIHTVPRSTLAESMFDKVRGVAARNNALLHDVSIESFAD
                     GLHVEQHIEVNETMSLIDAHHFVRGIEEEIRRELPQVKAVLTHIESEPATIEAPVVVE
                     RDRRIERLLRDAAANVPEIVDIHDVSVDRLGEHLHVTCHCTLPDAMEMRRVHDRITEV
                     EHRFKLDCPEVDRLLIHPEPASDNRHPQ"
     gene            3792..4193
                     /locus_tag="VHE33_10680"
     CDS             3792..4193
                     /locus_tag="VHE33_10680"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HVT97962.1"
                     /translation="MIASAVWKHDEQYEGKTQSGHAILMDATAEHTAGPSPMELVLTA
                     LCGCTSVDVVSILQKKREPLEGLTVSAEAEQAPAPPRTFTKIHLTYRIRGKVSRKAAE
                     DAVSLSKNKYCSVSKMLEKAATIDYSIEIES"
     gene            4190..4942
                     /locus_tag="VHE33_10685"
     CDS             4190..4942
                     /locus_tag="VHE33_10685"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HVT97963.1"
                     /translation="MTGFFRFVLLLLLLVVVAMTSAILTMHFAIHGAEVSIPDFKGMT
                     MADAGQRASAQGLTLQVESHLYSTGVPLGRVASQSPEPGTLVRRGWRVWLTESLGPQK
                     LAIPDVKGKDQRFATIAIRRAGFQVGNVAEIPWTGAQPGTVIAQSPDANAAGVESPTM
                     NLLVAAEPGPGTPESSGFVMPNFEGQLFTAAALAVTRIGLKLGPVREQDMHGVAAGPA
                     GPVVPPGTVVGQSPAAGDRVDATMTIQLTVAK"
     gene            4997..6382
                     /locus_tag="VHE33_10690"
     CDS             4997..6382
                     /locus_tag="VHE33_10690"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HVT97964.1"
                     /translation="MKELDRWVVAAALAAGAVGLAAQTAATLPMAPGAQVTTLSAQPG
                     TWSEPGVAVNPRNPQQVLAVFQYVAQAGYSNDAGRTWKLAQGVAAANYRMSGDVSATF
                     DDHGHALICFISFDKLGTVNYWAHGATRNGIFVRRSLDGGATWEPQLRTVVAQPTTPG
                     IPFEDKPYIVADTGEKSPYKGNLYIGWTRWTLTDSRMLFSRSTDDGVSWSNPQEIAQV
                     RGLPRDDNGALEGFDGAVGPDGVLYAVWAVTDHIVFTESDDGGKTFAKTRNIAHTAPT
                     MFALAGMERANGFPQIAVDSRTGPGGGAIYVTWSDYRNGDLDVFCIVSRDHGRTWSAP
                     VRVNNDPVHDGADQFFQWLAVDPVSGAVNVAFYDRRADAADKLQGMALARSTDGGATF
                     RNYAWTKTPFDPAGVFMGDYIGLAASGGRVYGAWTTRTGKEQKTSVELGVADFGNEQG
                     RLVQAPDKFSQ"
     gene            6460..7152
                     /locus_tag="VHE33_10695"
     CDS             6460..7152
                     /locus_tag="VHE33_10695"
                     /inference="COORDINATES: protein motif:HMM:NF006047.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HVT97965.1"
                     /translation="MLDLLKSEVLEANLELVRRGLVLYTFGNASGIDREQRLVVIKPS
                     GVAYDKLTPDDMVVSDLNGKIVGGKLKPSSDLDTHLELYREFPAIGGVVHTHSEFATA
                     WAQAGREIPAFGTTHADYFHGPVPVTEPLRDDEVGDDYVLNTGKAIVRRFRGLDPIAV
                     PGVLVAGHAPFAWGKNALDAAYHAVVLEAVARMAFYTTTLNPDGKGISQALLDRHHYR
                     KHGAKATYGQRS"
     gene            7223..>7772
                     /locus_tag="VHE33_10700"
     CDS             7223..>7772
                     /locus_tag="VHE33_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97966.1"
                     /translation="MSFAPFTVAAVLFQAQSLVHLSGVDIAVIAIYFAMVIYIGFYLK
                     GAANTSEDFFMAGREMTAWIAGLAFVSANLGSLELMGWAGSAYQYGILATHWYWIGAI
                     PAMLFLGIVMMPFYYISKTHSVPGYLHLRYGDGARGLSAVCFAFMTVLMSGINMYSMA
                     LVMKVVLGWDLNFSIWVSSITVA"
BASE COUNT         1470 a         2269 c         2595 g         1438 t
ORIGIN      
        1 cgacgccgag caggcggaag ggaatcagaa gcagccaaac aatgggatac gcgatcagcg
       61 ccagaagcgc caggggccag cagaggaaca gcagaatgca gaagagaagg aatttgatca
      121 tcgcgatgcc tcacttccca cgacagtatt acgcagaagc tagaaaagag gttcccgcat
      181 cgctcggccg agcttcagac gcctctgaac gccgatcgcc gaccacggaa cgccgatggc
      241 cgacaacgga acgccgatgg ccgacaacgg aacgctgatc tctgaccact gaacgctgca
      301 tctacggcat gtgaagaccg agagcgttga gaatagcccg gtcgggggtc gtgactccgt
      361 tctctttggt cacgacactg aatccgccct gatagtccca catggcccag ccaatgtggt
      421 tcttttccat cgcagtgcgc atgtcatgga gccactgggc gcgggccgcc ggcggagcaa
      481 actcccggaa gacgccaaac tcgccgcaat agaccggcgc atggtgaagg gccgaccact
      541 tggccgcgaa ttcgatcgag ttctccacgc gcgcggcgtc ccagcgattc tccccgtact
      601 cgtcgaggaa gtactggccg gcaaggtcgg gttcctgatt cagtttgggc gcgatgttct
      661 caggagttga aggataggga atagcgcgct cgggacggac ttccgggttg gtccatgttg
      721 ccccctgatg ggtgaagggg aagggttcgt aatcatggaa ggtgtaaatg acattgtcat
      781 cggcaagagt ctcggtcgcc aggagatcgg gcaagcccga ccagtgcgcg ccggaggcga
      841 tgatcgtgtt gtcaggggcg acttgccgaa tcgcctgcac cacctgggcc tcgatgccgt
      901 gccagcgcca cgggtcctgc tgctccgact cgttcatgat ctcgaagaag acgtgctctg
      961 gatcggcggc ggcgtaatgc ccggccaacg cacgccaaag ttccacgaag ttgccaatgc
     1021 cgccggctcc ctggaacagc tcggctttga actggctggt ggggtgaacg tcgatgatga
     1081 ccgagagatg attgtcgagc atcgtcttga cgacccggtc cagtgcctcc atgaagccgg
     1141 tggagtgatg cacatcgcgc tgggtgtaga accaggtcat gagctggtcc ccgtcaatag
     1201 agaggcggca gtgatcgaag cccattttgg cgatgagggc gatgtcgtca gcggtggtga
     1261 agctcctcag gcgctgaggc gaatagtcgc tcgcctgggc gaaccacatg ctggtgttga
     1321 tgccgtgctg gagatgcgcc gcgcgctcga acgctgtcga tgtccgggga ttctgtgcgg
     1381 aggcgagaag cggcaggaac agggagacga cgagggcagc gaagcagcgc ggacgaattc
     1441 gcatgagcgc agtgtaatcc cgcttccacg agggaaggtg gcgttgcggc tgcgctatgc
     1501 tgacgtttga caaaccgaag tcacaagccg gatccgaacg cggcgcacaa ccctggctcg
     1561 cggccggcgg gcgcggtcga tgaggccacc tcggcggcgc tggtgcggga gatgttcgac
     1621 cgcatcgctc ctcgctatga cctgctgaac catgtcctct cctgcaacgt ggaccggacg
     1681 tggtggtggc ggacggcacg gaccttccgg cacattttgg agcggcccga cgcgaaggtg
     1741 ctcgatattt gttgcggtac cggcgacatg acacgggctc tgctgcggcg caggcccggc
     1801 ggaaacgaaa aacaaccggc ggagccggtc atcgccgcgg atttctccga aaagatgctg
     1861 gcgctggcgc gagtcaagct tgccgggcgc caggtctcca tccagtcggt ggatgcgatg
     1921 cggatgccgt tcgcggatgg gtcgctcgat ctgatcacca ccgcgtttgg gtttcgtaat
     1981 ttgccgaact acaaagccgg cctcgaagag ttcctgcgcg tactcaagcc gggcggcgag
     2041 gtcgggatcc tggactgcag ccggcccggc ggtgtcctcg gcaaggggtt tggattctac
     2101 ttccggcacg tgctgccggc gattggagcg cgcatttccg gcgatgccgt cgcgtatgga
     2161 tacctgccca gttcggtgga gggctttccg ccgccgcccg cgatggtgga gctgatgcgc
     2221 agcgtgggat ttgtcgacgt gcagtggacg ccgtacacgt ttggaatcgc gggactgtgg
     2281 cggggaagaa agccgcctgg ctttcagtca tcacctctca gcgttcaggt aggctgagaa
     2341 ctgcatgatg tcgagcgcgg ccttgacgaa acaaacgcac gcccgcgcgg agaagcgatg
     2401 ggtgtcgctg gtctccgtga tggccgcctg cgcgatgacg gtgctgaagg cggcggtcgg
     2461 aatcatgacc ggaagcctgg gtgtgctgtc cgatgccgcg cattcaggtc tggacctcgt
     2521 gggcgcgctc atgaccttcc tgtcggtgcg ggtaagcgac aagccggcgg actggaacca
     2581 tccttacggc cacgccaaga tcgaaaacat ttcggcattt atcgagaccg gactcatggc
     2641 cgcctcggcg ttgtggtttt ccggtgaagc catcagccgc atgttctttc atcccgtgtc
     2701 gctgcgatat tcctggtggc ccttcgccgt cctggtaatc tccatggggg tggatctgtg
     2761 gcgctcggcg cagctgaggc gggtggcaaa gcaacaccaa agcgccgcgc tcgaggccga
     2821 tgcgctgcat ttcgcctccg acatctggac cagcgtggcg gtcgtgctgg ggctggcagc
     2881 ctcgtgggtg ggggtggtgc accatgtggg atggctgcgg tttgccgatc ccgcggccgc
     2941 cctggtggta tctgcgatga tcctggtctt cggatggcgg ctggcctggc gcgcggtcgc
     3001 agccctcacg gatcagattt cgcccgaact gcacacgaag atgatcgatg cggtgcgcga
     3061 gaccgacgga gtgctgggca tcgatcaggc gcgagtgcgg aaatcgggaa atcactattt
     3121 tgccgatctc acattgtccc tgccgcgcca gctcaccttt cagagcacag aagagctggt
     3181 ggagagagcg accggggcgg tgcagcgcgt cctgcccggc gccgatgtgg tgattcacac
     3241 ggtgccccgc tcgacgctgg ccgagagcat gttcgacaaa gtgcgcggag tagcggcccg
     3301 taacaatgcg ctgctgcacg atgtgagcat tgagtcgttc gctgacgggc tgcacgtgga
     3361 acagcacatc gaggtgaacg agaccatgtc cctgatcgat gcccaccatt ttgtgcgtgg
     3421 gatcgaagag gaaattcggc gggaactgcc ccaggtcaaa gctgtgctca cccacatcga
     3481 aagcgagccc gccaccatcg aggctccggt cgtggtggag cgggatcgcc gcatcgagcg
     3541 cctgttgcgc gatgcagccg ccaatgtgcc ggagatcgtg gatattcacg acgtcagtgt
     3601 ggaccggctg ggcgagcacc tccacgtaac ctgccactgc acgcttcccg acgcgatgga
     3661 gatgcggcgc gtgcatgatc gcatcacaga ggtcgagcat cgttttaagc tggattgtcc
     3721 ggaggtggac cggctgctga ttcaccccga gcccgcttcg gacaaccggc atccgcagtg
     3781 aggaggttgt tgtgatcgcc agcgcagtct ggaaacacga tgagcagtat gagggaaaga
     3841 cgcagagcgg ccacgcgatc ctgatggatg cgacggcgga gcacaccgcg ggtccgagcc
     3901 cgatggaact ggtcttgacc gcgctgtgcg gatgcacctc cgtcgacgtg gtgagcatcc
     3961 tgcagaagaa acgcgagccg ctggaaggcc tgaccgtgag cgcggaggcc gaacaggccc
     4021 cggcgccgcc gcgcaccttc acgaagattc atttgaccta tcggatcaga ggcaaggtaa
     4081 gccggaaggc tgccgaggat gcagtgtcgc tttcaaagaa caaatactgc tcggtttcga
     4141 agatgttgga gaaggcggcg acgatcgact actcgatcga gatcgaatca tgacgggatt
     4201 tttccggttc gtgctgttgc tgttgctgct ggtcgtggtc gcgatgacct cggcgatcct
     4261 gacgatgcat ttcgctatcc acggggccga ggtatcgatc cccgatttca aagggatgac
     4321 catggccgac gccgggcagc gggcatcggc acagggcctc acgctgcagg tggagagcca
     4381 cctctactcg accggcgtgc cgctggggcg cgtcgccagc cagtcgccgg agccgggaac
     4441 cctggtgcgc cgcgggtggc gggtgtggct gaccgaaagc ctcggcccgc aaaagctggc
     4501 gattccggat gtgaagggca aggatcagcg gtttgcgacg atcgccatcc ggagagcggg
     4561 attccaggtc ggcaacgtgg cggagatccc atggacggga gcccagccag gaacggtgat
     4621 tgcccaaagc ccggatgcca atgcggcagg tgtggagagc cctacgatga atctgctggt
     4681 ggccgcggag cctgggccag gcacacccga gtcgagcggc tttgtcatgc cgaactttga
     4741 agggcagttg ttcaccgcgg cagcgcttgc ggtcacgcga atcgggctga agctcggccc
     4801 ggtaagggaa caggacatgc atggggttgc cgcggggccg gcagggccgg tcgttccgcc
     4861 cgggaccgtc gtcgggcagt ccccggcagc cggcgaccgc gtggatgcca ccatgacgat
     4921 tcagctgacg gtggcgaagt agactgacag ttcgctggtc tcagatcaca gagtccgcgc
     4981 agagagaatg gtgcgcatga aagaactgga tcgttgggtg gtggcggccg ccttggctgc
     5041 cggcgccgtg gggctggccg cgcaaacggc agcgacgttg ccgatggcgc caggtgcgca
     5101 ggttacgaca ctctccgcgc agccgggcac atggtcggag ccaggcgttg ccgtgaatcc
     5161 gcgcaatccg caacaggtcc tggcggtatt ccagtatgta gcgcaggcgg gctactcgaa
     5221 cgacgcggga cgcacgtgga agctggccca gggcgtggcg gctgcgaact acaggatgtc
     5281 cggtgacgtt tcagcaacct ttgacgatca cggtcatgca ctcatttgct tcatctcgtt
     5341 cgacaagctg ggtaccgtca actactgggc acatggggcc acgcgcaacg gcatctttgt
     5401 gcgcaggtct ctcgacggcg gcgcgacctg ggagccgcag ttgcgcaccg tggtagcgca
     5461 gcccaccacg cccggaatcc cgtttgaaga caagccctac atcgtcgcgg acacagggga
     5521 aaagagcccg tataagggca acctctatat cggctggacc cgctggacgc tgaccgattc
     5581 ccggatgctg ttctcgcgct caaccgatga cggcgtgagc tggtcgaatc cacaggagat
     5641 cgcgcaggtc cgcgggctgc cgcgcgacga caacggagcg ctggaaggat tcgacggggc
     5701 ggtgggtccg gacggcgtcc tgtatgccgt atgggccgtg accgatcaca tcgtgttcac
     5761 cgagtccgac gatggcggaa agaccttcgc gaagacacgc aacatcgcgc atacggcgcc
     5821 gacaatgttc gcgctggcgg ggatggagcg ggcaaacgga tttccgcaaa ttgctgtcga
     5881 ttcgcgcaca ggtcctggcg gcggcgcaat ctacgtgacg tggagcgact accgcaacgg
     5941 cgatctcgac gtgttttgca tcgtatcccg cgatcacggg cgtacctgga gcgcgcccgt
     6001 acgagtgaac aacgatccgg ttcacgatgg agcggatcaa tttttccagt ggctggccgt
     6061 cgatcccgtc tcgggcgcgg tcaatgtagc gttttacgat cgccgggcag atgccgcgga
     6121 caagctgcaa gggatggccc tggctcggtc caccgatgga ggcgcaacct tcaggaatta
     6181 cgcctggacg aagacgccgt tcgatccggc cggcgtcttt atgggcgact acatcgggct
     6241 ggcggcatcg gggggacgcg tgtacggggc ctggaccacg cggactggaa aggagcagaa
     6301 aaccagcgtg gagctgggtg tcgcagattt tggcaatgag caagggcgat tagttcaggc
     6361 ccctgacaag ttttcgcagt aaacgctttc ccatccgcta tctgtcgagt agaatggcgc
     6421 tttgctccca ccgtcttttc cgcgggagcg tgtgacgcga tgctcgatct gctgaagtcc
     6481 gaagtgctcg aagcgaacct cgaactggtt cgccgggggc tggttcttta caccttcggc
     6541 aacgccagcg gcatcgatcg cgagcagcga ttggtggtca ttaagcccag cggcgtcgcc
     6601 tacgacaagt tgactcccga cgatatggtg gtgagcgatc tgaacgggaa gattgtgggc
     6661 ggaaagctga aaccgtcgtc cgacctggac acgcacctcg aactgtatcg ggagtttccc
     6721 gccatcggcg gcgtcgtcca tacccactcg gaattcgcga ccgcatgggc gcaggcgggc
     6781 cgcgagattc cggcgttcgg aacgacgcac gcagactatt ttcacggacc ggtacccgtg
     6841 acagagcctt tgcgcgatga cgaagtcggc gacgactatg tgctgaacac cggcaaggcg
     6901 atcgtgcggc ggtttcgcgg gctcgatccg attgcggtgc cgggagttct cgtggcgggg
     6961 cacgcgccgt tcgcctgggg caagaacgcg ctcgatgccg cgtatcatgc ggttgtgctc
     7021 gaagcagtgg cacgcatggc cttctacacg accaccctca acccggacgg caaagggatt
     7081 tcgcaggcct tgctggaccg gcaccattac cgcaagcatg gggcgaaagc cacgtacgga
     7141 cagagaagtt aacgaattcc tttaacctgg aaacagaccc ccatcggttt cccgaacgca
     7201 tggcatacga gggtcctgaa gcatgtcctt cgcaccgttc accgtcgcgg cagtgctttt
     7261 ccaggcgcaa tcgctggttc atctgagcgg cgtcgacatc gccgtcatcg cgatctactt
     7321 cgcgatggtc atctacatcg gcttctacct caaaggcgcc gccaacacca gcgaagactt
     7381 cttcatggcc ggccgcgaga tgacagcctg gatcgcaggt ctggccttcg tttcggccaa
     7441 cctcggatcg cttgagctga tgggctgggc cggctccgcc tatcagtacg gcattctggc
     7501 gacccactgg tactggatcg gcgcgattcc cgccatgctg ttcctcggca tcgtcatgat
     7561 gccgttctat tacatctcga agacccactc ggtgcccggc tatctccacc tgcgctatgg
     7621 cgatggcgcg cgcggcctgt ccgcggtgtg cttcgccttc atgaccgtgc tgatgagcgg
     7681 catcaacatg tactccatgg ccctggtcat gaaggtggtg ctcggctggg atctgaactt
     7741 ctccatctgg gtctcctcca tcaccgtcgc gg
//
LOCUS       DASZYN010000413         4933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_20150, whole genome shotgun sequence.
ACCESSION   DASZYN010000413 DASZYN010000000
VERSION     DASZYN010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20150"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="VHE33_10705"
     CDS             <1..1012
                     /locus_tag="VHE33_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97967.1"
                     /translation="EMTMHYGLLPRANRGIFAVNELPDLAGKIQVALFNIMQEGDVQI
                     KGYPVRLELDVALVFSANPEDYTARGKIVTPLKDRIGSEIRTHYPETLEEGIAITTQE
                     AWTEREQSDAVVPHYLREVIEQVAFTAREDKKVDKRSGVSQRLPISTLELAVSNAERR
                     ALLHSETRVVPRVGDLYTALPGITGKLELEYEGEMKGADTVVRDIVRAAIGKVFDRYF
                     RDVNTQQIEQWFNLGGSVKISDAQAAKVSFEELKQIQGLLEKLAPLGVKASDAPEFVT
                     AAAEFLLEGMCSHRRISRNEERSFSAQKKTPDRPERMERADPEREYEQWQTRRSRRGS
                     FN"
     gene            1037..2269
                     /locus_tag="VHE33_10710"
     CDS             1037..2269
                     /locus_tag="VHE33_10710"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVT97968.1"
                     /translation="MKSVRYTKFTGDLASEMSMEDLLKALSDYLLDSGFQNPFAEFQD
                     LDQTMEDLREALRRALEQGDAFDESIQQKIDQMSAEGKLDELVDKLIDRLERENYIST
                     TTSQQQGQTTQQAGQMGAANDARFEVTDKSLDFLGYKTLRDLLGSLGKSNFGRHDTRH
                     WATGVETSGGSQPYQFGDTLNLDGPATLKSAIAREGLTLPLNLEYSDLQVHQSEYQSS
                     CATVVMLDCSHSMILYGEDRFTPAKKVAMAMAHLIRTQYPGDTLSLVLFHDSAEELPV
                     SQLARVKVGPYYTNTREGLRMSQRILSRQRKDMKQIVMITDGKPSALTLPDGRIYKNA
                     FGLDPLVVSETLEEVARCKRQNIMINTFMLASDFGLVQFVQKVSQMCRGKAYFTTPET
                     LGEYLLMDFMQRRMKTIH"
     gene            complement(2281..>4933)
                     /locus_tag="VHE33_10715"
     CDS             complement(2281..>4933)
                     /locus_tag="VHE33_10715"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97969.1"
                     /translation="HDLDSQAAENLLQFLSDQQNVTATVPDDRCIVIERIRDELGDWR
                     VCVLTPFGNRIHTPWAMAAAAQVLAAGGPHVESMSTDDGFVLRFPDTDAAPDPMLLLP
                     KPDRAQDLVIHQLGSTALFAAKFREASARALLLPRRRPQIRAPLWQQRKRAADLLAIA
                     SRYPEFPMILEAHRECLRDAFDMPALLDVLRSIQEREIQVHVVDTTKASPFAASLLFG
                     YTGNFIYDGDAPLAERRAAALSIDQSLLADLLGEADLRELLDPVALAEVEANLQLLGE
                     NQRVRTADGLHDLLLRLGDLSRDEIAQRVSTPDLLKSLNSLVAARRILSARIGGQARY
                     IAVEDAARYRDALGLKLPDGIPKSLLAPVEQAVLEIVRRYARTHGPFTAREAAARFGL
                     DTAPVEATLRTLVHSGRVLEGGFRPQAAGREYCDAESLRLIRNKSLARLRKQIEPADP
                     HIFARFETHWQGVLQKRRGLDALLDTIESLQGTPLSASLLESQLLPARLGRYSPADLD
                     TLIAAGEIVWCGVDSIGERDGRIALYLADRITSLLPPQSAPSEPLSPREQAILDFLAR
                     SGATFFSDLHEATGGGYPGESVDALWSLVWRGLITNDTLHALRAFIARPETSRTSKRV
                     HNQPAFRSRRTTPPAAQGRWTLVPRPDRSTPAQQTAWSHAIAQQLLQRYGIVTRETAA
                     QEGVPGGFSAIYEVLKALEGQGKIRRGYFVAGLGGAQFGQPAAVELLRSLRASTPEQP
                     EMILLAASDPASPWGSILRWPVPDSAASDNAAVLTRSVGANVILRNGQLVAYLRRNNP
                     ALQVILPADEPDRSAAARDLAQFLGTIAQELLQAPETRHHGGLLIDAIAGRPVHEHFL
                     ASFLLDAGFHPSPRGFHVRYVPAAADR"
BASE COUNT         1078 a         1415 c         1578 g          862 t
ORIGIN      
        1 cgaaatgacc atgcactatg gcctgttgcc gcgtgcgaat cgtggcatct ttgccgtcaa
       61 tgagctaccg gacctcgctg gaaagattca ggtggctctg ttcaacatca tgcaggaagg
      121 cgatgtccag attaagggtt atcccgtgcg gctcgaactc gatgtggccc tggtcttcag
      181 cgccaatccg gaggattaca cggcccgcgg aaagatcgtg acgcccctca aagaccgaat
      241 cggctcggaa attcgtaccc actatcccga aacgctggaa gagggaattg cgattacaac
      301 ccaggaggca tggaccgaac gggaacagag cgatgctgtg gtcccccact acctgcgcga
      361 ggtgatcgag caggtggcat tcacagcccg cgaagacaag aaggtcgaca agcgcagcgg
      421 tgtaagtcag aggcttccga tctccacttt ggaactggca gtcagtaatg ccgaacgccg
      481 ggctctgctg catagcgaga ctcgcgttgt ccctcgggtc ggtgatttgt acaccgcgct
      541 gcccggaatc accggcaagc tggaactgga atacgaaggc gaaatgaaag gggcggacac
      601 ggtcgtgcga gacatcgtgc gcgcggccat tggcaaggtc ttcgaccggt acttccgcga
      661 tgtgaacacg caacagatcg aacagtggtt caacctgggc gggtccgtaa agatctccga
      721 cgcgcaggcg gcgaaggtca gtttcgaaga gttgaagcag attcaggggc tgctcgaaaa
      781 gctggctccg ttgggcgtga aagcgagcga tgcacccgaa tttgtgacgg cggccgctga
      841 gtttcttctg gaaggaatgt gttcgcatcg acggatcagc cggaacgagg agcgtagctt
      901 cagcgcccag aaaaagacgc cagaccggcc cgagcggatg gaaagggccg atccggaacg
      961 tgagtacgag cagtggcaaa ccaggcgatc gagacgagga agttttaact agccattcgc
     1021 ggcttcaggt cttgcgatga aaagcgttcg gtacacaaaa ttcacagggg atctggcgag
     1081 cgagatgagc atggaggacc tgctcaaggc gctctccgat tacctgctgg actccggatt
     1141 tcaaaatcca tttgccgagt ttcaggacct cgatcagacc atggaggatc tgcgcgaagc
     1201 gctgcgccgc gcgctggagc agggagatgc cttcgacgag agcatccagc agaagatcga
     1261 tcagatgtct gccgagggta agctggatga actcgtcgat aagctgatcg accggctgga
     1321 gcgggaaaat tacatttcga cgacgacgtc gcagcagcag gggcaaacaa cacaacaagc
     1381 cgggcagatg ggcgccgcga acgatgcgcg attcgaggtg acggataaga gcctcgactt
     1441 cctgggatac aaaacactgc gcgatctgct cggatcgctt ggcaagtcga atttcggacg
     1501 tcacgatacg cggcattggg cgaccggtgt ggaaacaagc ggcgggtctc agccatatca
     1561 gtttggagac accctgaatc tggatggacc ggcaaccctc aagagcgcga tcgcgcgcga
     1621 ggggcttacg ctcccgctga atctggagta cagcgatctg caggtgcatc agagcgagta
     1681 tcaaagctcg tgcgcaaccg tggtgatgct cgattgctcg cactcgatga tcctgtacgg
     1741 tgaagaccgc tttacgccgg ccaagaaagt tgccatggcc atggcgcacc tgatccgcac
     1801 ccaatatcca ggagacacgt tgtccctcgt gctcttccac gactccgccg aagaattgcc
     1861 ggtttcacag ttggcgcgtg tgaaggtcgg cccctactac accaacaccc gcgagggttt
     1921 gcgcatgtcc cagcgcattc tctcccggca gcggaaagac atgaagcaga ttgtgatgat
     1981 caccgatggc aagccttcgg cactgaccct gcccgatggt cggatctaca aaaacgcctt
     2041 cggcctcgat cccctggtgg tcagcgagac gctggaagaa gtggcccgct gcaagcggca
     2101 aaacatcatg atcaatacct tcatgctggc gtcggatttc gggctggttc agtttgtgca
     2161 gaaggtctcc cagatgtgcc ggggaaaagc ctacttcacc acgccggaaa ccctgggcga
     2221 gtatctcctc atggatttca tgcagcggcg gatgaagacc attcactagc ccgcctgcgg
     2281 tcagcggtcg gccgcagccg gaacatagcg cacgtggaac ccgcggggcg aaggatgaaa
     2341 ccccgcgtcg agaagaaacg atgcgaggaa atgctcgtga acgggtcgtc ccgcaattgc
     2401 gtcaatcaga aggccgccat ggtgccgcgt ttccggcgcc tgtaacagtt cctgagcaat
     2461 cgtgccaaga aattgcgcca gatcgcgggc ggcggcactg cggtcgggtt catccgcggg
     2521 cagaatcacc tgcagcgcgg gattgttgcg ccgcagatac gcgacaagtt ggccattgcg
     2581 caaaatgaca ttcgctccaa cactgcgggt cagaacggca gcattgtcgg atgccgcaga
     2641 gtctggaaca ggccagcgca gaatactgcc ccaggggctc gcagggtcag aggcagccag
     2701 caaaatcatc tcgggttgct cgggcgtcga cgcgcgcagc gaccggagca gttccaccgc
     2761 agccggctga ccgaactgag cgcctcctaa acctgcgaca aaatagccgc gacggatttt
     2821 gccctgccct tcaagagcct tgagaacctc atagatcgcg gagaagccgc cggggacccc
     2881 ttcctgagcg gccgtttcgc gggtgacaat tccgtatcgc tggagcagtt gctgagcgat
     2941 cgcatgcgac cacgcggtct gctgcgccgg cgtggagcgg tcaggacgag gtaccagagt
     3001 ccagcggccc tgcgccgccg gaggcgtggt gcggcgggat cggaacgcag gctgattgtg
     3061 gacgcgtttg ctggtccgcg acgtctcagg cctggcgatg aacgcgcgca gggcatggag
     3121 cgtatcgttc gtaatcagcc cgcgccagac cagagaccac agcgcatcga cgctctcccc
     3181 ggggtagcca ccgccggtcg cttcatggag atccgagaaa aaggttgcgc ccgaccgcgc
     3241 aaggaaatcg agaatcgcct gttcgcgagg cgaaagcggc tcgctgggtg cgctctgtgg
     3301 cggcaacagt gacgtgattc gatcagcaag ataaagtgca atgcgcccgt cccgttcccc
     3361 gatgctgtcg acgccgcacc agacgatttc tcctgcggcg atcaatgtgt ccaggtccgc
     3421 gggcgaataa cgcccgaggc gtgccggcag cagctgggat tcgaggagag acgcggaaag
     3481 aggcgtgccc tgtagcgact cgatcgtgtc gagcagagca tcgaggcctc ggcgcttttg
     3541 cagaacgccc tgccaatgag tttcaaaccg agcaaaaatg tgcgggtccg cgggttcgat
     3601 ctgctttcgt aaccgtgcca gtgacttgtt gcggatcagg cgcagcgatt cagcgtcgca
     3661 gtactcgcgc cccgccgcct gagggcgaaa tcctccctcc agaacccggc cgctgtgaac
     3721 aagagttcgg agcgtagctt cgaccggtgc tgtgtccagt ccgaatcgcg cggccgcctc
     3781 gcgcgcagtg aaaggcccat gtgtgcgggc gtagcggcgg acgatttcga gaacagcctg
     3841 ctccaccggc gctaaaagag attttggaat tccgtccggg agcttcagac cgagtgcgtc
     3901 acgataacga gcggcatcct cgacggcaat gtagcgcgcc tgtccgccaa tcctggccga
     3961 aaggatgcgg cgtgcggcca ccagagaatt cagggacttg agaaggtccg gagtcgacac
     4021 acgctgggcg atttcgtcgc gcgacaggtc gcccaggcga agcaacagat cgtgcaaccc
     4081 atccgccgtg cggacccgct gattttcgcc gagcagttgc aggttcgctt cgacctccgc
     4141 gagagccacg gggtcgagca gttcgcgaag atcagcctcg ccgagcaggt ccgcaagcag
     4201 tgactgatca atggagagcg cggcggcgcg gcgttcggcc agtggcgcat ccccatcgta
     4261 aataaagttg cctgtgtagc cgaaaagcag cgatgccgcg aacggcgatg ctttcgtcgt
     4321 atcgacgaca tgcacctgaa tctcgcgctc ctgaatggag cgcagcacgt cgagtagcgc
     4381 cggcatgtcg aaagcatcac gaaggcactc gcgatgagcc tcaaggatca tcgggaattc
     4441 gggatagcgc gaagcgatgg ccagtaggtc tgcggcacgc ttgcgctgct gccacagcgg
     4501 tgctcggatc tgagggcggc gccgcggaag gagcagagcg cgagcagagg cctcccggaa
     4561 ttttgctgcg aatagcgccg tcgagccaag ctggtgtatc accaggtcct gcgcgcgatc
     4621 gggcttcgga agaagaagca tggggtcggg agcggcatcg gtgtcgggaa accgcaggac
     4681 gaagccatcg tccgtggaca tggattccac gtgagggccg ccggctgcga ggacttgcgc
     4741 cgccgccgcc atggcccagg gcgtgtggat ccgattgccg aacggagtca ggacacaaac
     4801 acgccagtcg cccagctcat cgcggatgcg ctcgatcacg atgcagcggt catctggcac
     4861 agtggcagtc acattctgct ggtcgctgag aaattgaagc agattctcag ctgcctggga
     4921 gtcaagatcg tgt
//
LOCUS       DASZYN010000414         2544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_148233, whole genome shotgun sequence.
ACCESSION   DASZYN010000414 DASZYN010000000
VERSION     DASZYN010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148233"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VHE33_10720"
     CDS             <1..682
                     /locus_tag="VHE33_10720"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HVT97970.1"
                     /translation="HFPQSTVPGHSGKLVIGKVAGVPVAVMQGRVHAYEGYNSDEVVF
                     PIRVLAAMGVTQLVLTNAAGGINPAFQRGQLVLIADHINLSGRNPIAGPADERMGPRF
                     FDMSEAYSGRLRTVAHEAGHEEGYRLDEGVYLSVLGPSFETPAEIRAFRTLGADLIGM
                     STVQETIAARQTGIEVLGISCVTNLASGLGAEPLSHEEVMETGRAVEKQLAGLLTRLM
                     PMMGAVSQ"
     gene            complement(717..1979)
                     /locus_tag="VHE33_10725"
     CDS             complement(717..1979)
                     /locus_tag="VHE33_10725"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HVT97971.1"
                     /translation="MKPFRPSALALFCLFSVPLSHAWGSKGHRIINRLAAQALPADIP
                     AFLRAPDAINEIDYLGPEPDRWRSRAEPELNAAQAPDHFIDLEYADLIGTLPRERYEY
                     IAALYAYAAAHPDRAAVLRPEKVGFQPYITSEVWERLKSAMRDYRTLSAQHQDTKPVE
                     AAILFYAGWLGHYVGDGSQPLHVTIQYNSWVGPNPNGYTTEHHIHSLFETTFVNAAIN
                     ASDVQPLLASPRPIGDEWTDYLAYLRRTGTYIEKVYQLDKAHGFDGSGTEESRQFTAE
                     RLAAGASMLRDMIVAAWEKSAEPVPEWHEEHALASPGAAANVSPDQVFRKPFPAGFRN
                     PILNYAPDPQFSDKARKHKYSGRIIVGLTVDKNGNATDVHIVSGENKELQQGALDAVR
                     QYKFSPATYKGEPVAAQIDVEVNFRTYE"
     gene            complement(2133..>2544)
                     /locus_tag="VHE33_10730"
     CDS             complement(2133..>2544)
                     /locus_tag="VHE33_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HVT97972.1"
                     /translation="ADVFALYLKTKNFHWHMSGPHFRDYHLLLDDHADQIFAMTDDIA
                     ERVRKIGGTTLRSIGQIARLQRIQDNDADFVTAQDMLSELHEDEKALVLSLRACHTLC
                     DDAEDVATASLIENWIDQTQRRAWFLFECTRAER"
BASE COUNT          484 a          679 c          888 g          493 t
ORIGIN      
        1 gcactttccg cagtcgaccg tgcccggaca cagtggcaag ctcgtcatcg gcaaagtcgc
       61 cggtgtaccg gtggccgtga tgcagggacg cgtccatgcg tatgagggat acaactcgga
      121 cgaagtcgtc tttcccatcc gggtgctcgc ggcgatgggc gtgacccaac tggtgctgac
      181 gaatgcggcg ggcgggatca atcccgcatt tcagcgcgga caattggtcc tgatcgcgga
      241 ccacatcaac ctgtccggac ggaaccccat tgccggtccc gcagatgagc gcatgggacc
      301 gcggttcttc gacatgagcg aggcgtactc cgggcggcta cggacggtgg cgcacgaggc
      361 ggggcatgag gagggctatc gcctcgacga gggcgtgtat ctgagcgtgc tcggcccaag
      421 cttcgagacg cccgccgaga ttcgggcctt ccggacgctg ggcgcggacc tcatcggcat
      481 gtcgacagtg caggagacga tcgcagcacg acaaacgggg atcgaagttc ttgggatcag
      541 ctgcgtgacg aatctggcgt ccggccttgg tgccgaaccc ctgagtcacg aagaggtcat
      601 ggagacaggg cgagcagtgg agaagcaact ggccgggctg ctaacgaggt tgatgccgat
      661 gatgggcgcg gtgtcacaat gacagccgca atcgttgtca gaaaccgcgg aacgtctcat
      721 tcgtaagtgc ggaagttaac ttcgacgtca atttgcgcgg caacgggctc tcctttgtaa
      781 gtagccgggc tgaatttata ctgccgtact gcgtcgagag cgccttgctg gagctccttg
      841 ttctcgccac tcacgatgtg gacgtcagtt gcgttcccgt tcttatcgac agttaagcca
      901 acgatgattc ttccgctgta tttatgtttg cgcgccttat cggaaaattg cggatcgggt
      961 gcgtagttca gaatcggatt acggaatcca gctggaaaag gctttcggaa aacctggtcg
     1021 ggcgagacgt ttgcggctgc gccaggtgat gcgagcgcat gctcctcatg ccactctggt
     1081 accggttcgg cgcttttctc ccaggcggca acaatcatgt cccggagcat cgacgccccc
     1141 gcggcgaggc gctcggcggt gaactgacgc gattcttccg tgccggatcc gtcgaagccg
     1201 tgcgccttgt cgagttggta gaccttctcg atatacgtgc cagtgcggcg caggtacgca
     1261 agataatcgg tccattcgtc gccgatgggg cgcggcgagg ctaaaagcgg ctgaacgtcc
     1321 gacgcgttga tggccgcgtt gacgaaggtg gtttcgaaca ggctgtggat gtggtgctcg
     1381 gtcgtgtagc cgttgggatt gggcccaacc cagctgttgt actgaatcgt cacatgcaga
     1441 ggttgcgagc cgtccccgac atagtggccg agccagccgg cgtagaagag gatcgcagct
     1501 tcgacgggct tcgtgtcctg atgctgcgcg gagagcgtgc ggtagtcgcg catggcggac
     1561 ttgaggcgct cccagacttc ggacgtgatg tacggctgga atccgacttt ctcaggccgg
     1621 aggacagcgg cgcgatcggg atgtgcggcc gcgtaggcgt agagggcggc gatgtattca
     1681 tagcgctcgc ggggcagcgt cccgatgagg tcggcatact ccagatcgat gaagtggtcg
     1741 ggggcctggg cggcgttgag ttcgggctcg gcgcgcgagc gccagcggtc gggctcgggg
     1801 ccaagatagt caatctcgtt gatcgcgtcc ggagcacgga ggaaagcagg gatatcggcg
     1861 ggaagggcct gcgccgcgag ccgattgatg atgcgatgcc ctttgctgcc ccaggcatgg
     1921 gacagcggaa cggagaacag acagaagaga gcgagagcac tgggccggaa gggtttcatc
     1981 aaaggtgagt atacgaaagc cgcgttgagc ggatggcgaa tagcgtttgg caaaaggggg
     2041 actgccgatc gcgcgggcgc gccatgccca ctctgattac gcgaaatgcg cgcgcaatca
     2101 gaatgggcgc ccgggatggg ctcgatgcgg aattaccttt ccgcgcgggt gcattcgaag
     2161 aggaaccagg cgcggcgctg ggtctggtca atccagttct cgatgagcga ggcggtagca
     2221 acgtcttcag cgtcgtcgca cagggtgtga caggcgcgca ggctgagcac cagggccttt
     2281 tcgtcctcgt gcagttcgct gagcatatcc tgggcagtga caaagtcggc gtcgttgtcc
     2341 tggatgcgct gcagccgggc gatctggcca atggagcgga gtgtggtacc gccgatcttc
     2401 cggacgcgct cggcaatgtc gtcggtcatg gcgaagatct gatcggcgtg gtcatcgagg
     2461 agaagatggt agtcgcgaaa gtggggaccg ctcatatgcc agtgaaagtt cttcgtcttg
     2521 aggtagaggg cgaagacgtc ggca
//
LOCUS       DASZYN010000415        10353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_659927, whole genome shotgun sequence.
ACCESSION   DASZYN010000415 DASZYN010000000
VERSION     DASZYN010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 10353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10353
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659927"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VHE33_10735"
     CDS             complement(<1..470)
                     /locus_tag="VHE33_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVT97973.1"
                     /translation="MRIASLQPSISIILDRLGCLHQLVACTRYCLEQVPALRDQNLTI
                     VHDSWSTTADELLAVRPDIVIASVPYRNESLAAILKAGCPVLALAPHSLADIAQDIRL
                     IAAVAGVPALGDQLVAEMTDAVAQMRTYTADLPQPRVYCEEWGKPLIHSQLWVR"
     gene            complement(474..1208)
                     /locus_tag="VHE33_10740"
     CDS             complement(474..1208)
                     /locus_tag="VHE33_10740"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HVT97974.1"
                     /translation="MSLETGPVARLTFPELQHAAFSPAPKIAVFDCDGTLWEGDTGNG
                     FMYWSIEAGVLSRDAGGWIDARHRLYRQGQVSEAQICGEMVQVYAGLREDELRRAAAR
                     YFSTQVETQIFPEIQSLVAGLRAAGADIWAVSSTNNWVVEEGVRRFGIPADHVLAASV
                     HVRNGLITSDLIDVPTDEGKALALERSGMPHPDQVYGNSIHDAAMLSIAARAFPINPT
                     PALAEIAAQRGWQVYYPEAILADHKK"
     gene            1592..4717
                     /locus_tag="VHE33_10745"
     CDS             1592..4717
                     /locus_tag="VHE33_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVT97975.1"
                     /translation="MRFALTDPPVVLSADASLEELTGFSAEDWISSRVCLQDRIHPED
                     KELLDGILSPELAEPCGYADLRIRHADGRIRPLHVSFTKERATDGTILQLQLTRPCAA
                     VSPELESIAHCFGTLMDHTDDAMYLKNEDQVYVRVTRAMARLLEDPGAKLDFAGKTAY
                     DVYPETLADALFRRDEDVFKGGRDTHEVQRVQVPDGSHRWIDNRKYPLKSEDGRVVGL
                     FGICPDITEPIDAWRKLGETNELFQLFIEYAPAAIAMFDREMRYIAASRRWIQDYGLA
                     GQDLTGRSHYEIFPEIPERWKALHQRALNGEDLYCDEDRFVRADFTVQWLRWELHSWR
                     TAEGSIGGLLIFTEDITQQKKDKEQLQLAASVFTNAREGIVICDPGGAILDVNEMFTR
                     ITGYSRAEALGRNPRLLKSGRQSEEFYADMWRSLTEKGNWSGELWNKAKDGRIYPEAL
                     TINAVYDRTSKVQHYVAHFTDISEMKEQARQLERMAHFDALTNLPNRALLTDRLQQAM
                     AQARQRGQMLAVVSIDLDGFRLVNEGYGQDAGDQLLMGVAQRMKQMLPETDSLARIGG
                     DEFVAVLQDLANADAVKPMMRKLLACASEPEICGENSVQVSASIGVVLYPQAEEIDAE
                     QLLRQAGQAMYQSKLAGKNRYQIFDPTHDITVRTFHEDLEQIGRGLAADEFVLHYQPR
                     VHMSTGALMGAEALIRWNHPTRGRLLPAEFLPVIESHPLIDRIGEWVIDHALTQLERW
                     NAAGLKIPVSVNIAAHHLQQPDFTDRLRALLAEHAEISPSRLELEVLETSALRDVAQV
                     SGVLAACRDLGVSIALDDFGTGYSSLTYFKRLPVNTLKIDRSFVRDVLDDPEDLSILE
                     GVLGLATAFDRMAIAEGVETADHGRLLLQLGCVYGQGNGIALPMPGDELPGWSSTWNP
                     DPMWVNVASVAPRKRSLMHAAVAHRAWVAGLEGFLQGTRRTPPTLDPHRCRLGRWLDS
                     ERDHPMGSSAAFRRIEQLHQETHAFADVASAWEPSATGADVSTQVKELHRLRDALIEQ
                     MNSLLQDTAAQ"
     gene            complement(4755..5096)
                     /locus_tag="VHE33_10750"
     CDS             complement(4755..5096)
                     /locus_tag="VHE33_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97976.1"
                     /translation="MRSVVLIHGLLQGNGRQEPCEMLAIRETPEKAGPAVYSRCSVID
                     APHDLPDGEYAVTFGGNIVPAKKEAGLWIPDKSNAAALAPSSEKSAPSRPSFNIAEAG
                     EILSVLKNRVA"
     gene            5257..5967
                     /locus_tag="VHE33_10755"
     CDS             5257..5967
                     /locus_tag="VHE33_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HVT97977.1"
                     /translation="MAGFRERLGQVEEKIGAACHRAGRPREEVRLMGVSKMHPAEALA
                     EAAGAGLILFGENRVQEFEAKRLRIAELGLEGLEVHLIGHLQANKTGKAAEMFDGVDT
                     VDSLRVAERLNDAAGKLGKSLPVLVEVKLSDEPSKAGVAPEDPELSVMLERMPELSHL
                     AMRGLMTIAPLDENPETARACFRRLRTLRDELARKFGRVNFDELSMGMSGDFEIAIEE
                     GSTLVRIGTALFGARALP"
     gene            5964..6284
                     /locus_tag="VHE33_10760"
     CDS             5964..6284
                     /locus_tag="VHE33_10760"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HVT97978.1"
                     /translation="MTSRVPVRETAQGASFLVRVMPRASRTAIVGVLGDGTDAAVKVA
                     LQAPPVEGRANAALIEFLAELLGVARSAIEIAGGETARTKRIVVRGKDAMEVATAIAI
                     ALTH"
     gene            complement(6322..7200)
                     /locus_tag="VHE33_10765"
     CDS             complement(6322..7200)
                     /locus_tag="VHE33_10765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HVT97979.1"
                     /translation="MASTVWKGYISFGLISVPIRLFVAARENHISFNQIHQVCGSRIK
                     QQTFCPTCERLVERSELVKGYPVDKENYLIVSSEELKGLEAQSSETMEIVQFVSLPEV
                     DPLYYQTSYFSVAEDPGRKAYSLIHKGMQDLKLGAIAKITLHQREQIVMIRPFEKGLV
                     LHTLYYPEEIRAVAEYDNQPEVETNKAEVALAEQFMQQLTAAFNPEKMKDEYTARVEQ
                     LIESKQGQAPAPGKQPKKKLAPVIDLMEALRKSMGQASPASVTASAAPQPDPDKDKKP
                     PQRVASKTAAKKKKAG"
     gene            complement(7510..8466)
                     /locus_tag="VHE33_10770"
     CDS             complement(7510..8466)
                     /locus_tag="VHE33_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVT97980.1"
                     /translation="MTAAKATRKPAPKPEVARHVDDFLAMLAHERNASPHTARAYDRE
                     LHNFAAFIVEKLGAGIAPKQIEHQHIRAYLSELYGRGLSKASVARALAAIRSWFKWLA
                     RHGLVEQSPAALVSTPKLPKHLPRVPTIEDMNRVVESAADDAAAWPERDRVIFELLYG
                     CGIRNAELVGIDLADIHWANDAILVRGKGRKERYVPLGDAAAQALRAYLPLREQKLAA
                     AQKSSPALLLNLRLRGDARLTTRSVGRIVKHIALAHGLPADVHPHTLRHAFGTHMLEE
                     GADLRAIQELLGHERLSTTQRYTQLTVGQVAEVYDRTHPRAK"
     gene            complement(8463..9410)
                     /locus_tag="VHE33_10775"
     CDS             complement(8463..9410)
                     /locus_tag="VHE33_10775"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HVT97981.1"
                     /translation="MSAPPKANRQASTPNSTNSALIREYLAYLRVEKGLRPLSCEAYE
                     RDLLQFAEFLESESVTLLAASQQQVAGFLAHLSKHSVESRSAARKLSCLRGFFRWLLL
                     DKRIRRDPTLNLESPSTWKVLPKSLAVSEVDSMMERAALAADHPQADAIALRDRAILE
                     LLYAAGLRVSELTGLNVADLSLDAGRALVRGKGDKERIVPLGRASIEALERYLQHGRP
                     ALEKKSSGRLFLSTRGLPLSRAWVWHLVKSSEQTASPHMLRHSCATHMVERGADLRTV
                     QTLLGHADIATTQVYTHLALGRLKAVHREHHPRAKRVTS"
     gene            complement(9498..>10353)
                     /locus_tag="VHE33_10780"
     CDS             complement(9498..>10353)
                     /locus_tag="VHE33_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97982.1"
                     /translation="LLFRLRRFGETEIGLGFTVILAAASLTAQSPSVDETPQDTLTAH
                     QVVDRMEWRWPSFHTPALAQLAPPTPLSVAVREATYTGGSASDANDRAWSEYNHHWAG
                     LIVLIAGLLALVSRLPRQTWARYWPLLFLGLAIFILLRADPECWPLGPRPFWASFTAP
                     DVLQHRLCALIIAVFAVFETGVQTGRFRSSAARSVFPMLCAVGGALLLTHTHALGEGN
                     DEVFAELSHTPMAVLGAVAGWSRWLELRLDNATLADPPSRERRIAAWIWPACLILVGI
                     LLLNYRES"
BASE COUNT         1895 a         2645 c         3904 g         1909 t
ORIGIN      
        1 tcgcgaaccc agagctggga gtggatcagg ggcttgcccc actcctcgca gtagacgcgg
       61 ggctggggca ggtcggcggt gtaggtgcgc atctgggcga cggcgtcggt catctcggca
      121 acaagctgat cgcccagggc ggggacgccg gcgacggcgg cgatgaggcg gatgtcctga
      181 gcgatgtctg cgagggagtg cggggcgaga gccagcacgg ggcagccggc tttcaggatg
      241 gcagccaggg attcgttgcg gtacgggacc gaggcgataa cgatgtcggg gcggacggca
      301 aggagctcgt cagcggtggt ggaccaggaa tcgtggacga tggtgaggtt ttggtcgcgg
      361 agggccggca cttgttcgag gcagtagcgg gtgcaggcga cgagctggtg gaggcagccg
      421 aggcggtcga ggatgatgct gatgctcggc tgcagggagg cgatgcgcat ggtctatttc
      481 ttgtggtcgg cgaggatggc ctcggggtag tagacctgcc agccgcgctg agcggcgatt
      541 tcagcaagtg cgggcgttgg gttgatcggg aaggcgcggg cggcaatcga gagcatggcg
      601 gcatcgtgga tggaattgcc gtagacctga tcgggatgag gcatgccgga gcgctcgaga
      661 gcgagggctt tgccttcgtc ggtagggacg tcgatgaggt cggaggtgat gaggccgttg
      721 cgaacgtgaa ccgacgcggc gaggacgtgg tcggcgggga tgccgaagcg gcggacgcct
      781 tcttcgacga cccagttgtt ggtggagctg acggcccaga tgtcggcgcc ggcggcacgg
      841 aggccggcaa cgaggctttg gatctcaggg aagatctgcg tttcgacctg ggtggagaag
      901 tagcgagcgg cggcgcggcg gagctcatct tcgcgaaggc cggcgtagac ctggaccatc
      961 tcgccgcaaa tctgcgcttc ggagacctgg ccctggcgat aaaggcggtg gcgggcgtcg
     1021 atccagccgc cggcatcgcg ggagaggaca ccggcttcga tggaccagta catgaagcca
     1081 ttgccggtgt cgccttccca gagggttcca tcgcaatcga agacggcgat tttgggggct
     1141 ggggaaaagg cggcgtgttg caattcggga aaggtcaggc gggcgaccgg cccggtttcg
     1201 agggacatca acggtactgt tctccttcca ttaccctgtc gacttttgta accacggctg
     1261 ctacggattc tgacggtctc gccagggctc tgctcgtagt atcgccgatg ggacgttgca
     1321 attgattgaa aaacggcatg ttggggcgga cgcgaggact ggccgatgaa ggccgccgtg
     1381 cgaatggggg gaggactctc gcagactgtg cgggctcgag atgctcgcaa cgcttcgcgc
     1441 agcgaaaagc cactctaact gcaaagcgac agtaacttac tggcggattt gtatttgcac
     1501 ttagcgcgcc gataaacctt gacggtggag tatcagctaa cggtatgctt cggcaggcag
     1561 ggaacgaccg ggcgccagga gctggaacac gatgcgattc gcactgaccg atccgcctgt
     1621 cgttctgtcg gccgatgctt cactggaaga gctgaccgga ttttccgcag aggactggat
     1681 aagttctcgt gtttgcctgc aggatcggat tcatcccgaa gataaggaat tgctcgacgg
     1741 gatcttatcg cccgagttag cggagccctg tgggtacgcc gatctgcgaa tccggcacgc
     1801 ggacgggcga atccggccgc tgcatgtcag cttcacgaaa gagcgcgcga ccgacggcac
     1861 gattctgcaa ctgcagctga cacgtccctg tgccgccgtg agtccggagc tggaatcgat
     1921 cgcgcactgt ttcggcacgc tgatggatca cacagacgat gcgatgtacc tgaagaatga
     1981 agatcaggtg tacgtccggg tgacgcgagc catggcaagg ctgttggagg atccgggcgc
     2041 gaaactcgat ttcgcgggaa agacggcata tgacgtttat ccggagaccc tggccgatgc
     2101 tctcttccgg agagacgagg acgttttcaa aggaggaaga gacacccacg aggtgcagcg
     2161 ggtgcaggtg ccggacggat cgcatcggtg gatcgacaac cgaaagtatc cgctgaagag
     2221 cgaggatggg cgagtggtgg ggctgttcgg gatttgcccg gacatcaccg agccgatcga
     2281 tgcgtggcgc aagctggggg agacgaacga actgttccag ctgttcatcg aatatgctcc
     2341 ggcggcgatt gcgatgttcg accgcgagat gcggtacatc gcggcgagcc ggcgatggat
     2401 tcaggattac ggactcgcgg gccaggacct gacgggacgg agccactacg agatttttcc
     2461 ggagattccg gagcggtgga aggcgctgca tcagcgtgcg ctgaacggcg aagatctcta
     2521 ctgcgatgag gaccggtttg tgcgggcgga tttcacggtg cagtggctgc gctgggaact
     2581 gcattcgtgg cgcacagcgg aggggagcat cggagggctg ctgattttca ccgaggacat
     2641 cacgcagcag aaaaaggata aggaacagct gcagctggcg gcaagcgtgt tcacgaacgc
     2701 acgggaaggg attgtgatct gcgatccggg gggcgcgatt ctggacgtga acgagatgtt
     2761 cacgcggatc accggataca gccgggcgga ggcgctgggg cgaaatccgc gtctgctgaa
     2821 gtcggggcgg cagagcgagg agttttacgc cgatatgtgg cggtcactga ccgagaaggg
     2881 caactggtcc ggcgagttgt ggaataaggc gaaggacggg aggatctatc cggaggccct
     2941 gacgatcaac gccgtctacg atcgcacttc aaaggtgcag cactacgtgg cgcatttcac
     3001 cgatatctcg gagatgaagg agcaggcacg ccagctggag cggatggcgc acttcgatgc
     3061 gctgacgaat ctgcccaatc gcgcgttgct gacggaccgg ctgcagcagg cgatggcgca
     3121 ggcacggcag cgcgggcaga tgctggcggt cgtatcgatc gatctggacg ggttccggct
     3181 ggtgaacgaa ggctatggcc aggatgccgg ggaccagttg ctgatgggcg tggcccagcg
     3241 gatgaagcag atgctgccgg agacggactc tctggcccgg atcgggggcg acgagtttgt
     3301 cgccgttctg caggacctgg ccaatgccga tgccgtgaaa ccgatgatga gaaaactgct
     3361 ggcgtgcgcg tcggagcccg agatatgcgg ggagaacagc gtgcaggttt cggcgagcat
     3421 tggcgtggtg ctttatccgc aggcggaaga gatcgacgca gaacagctgc tgcggcaggc
     3481 cgggcaggcg atgtatcagt cgaagctggc tgggaagaac cggtatcaga tcttcgatcc
     3541 gacgcacgac atcacggtgc ggacatttca tgaggatctg gagcagatcg ggcggggcct
     3601 ggcggcggat gagttcgttc tgcactatca accgcgcgtc cacatgagca cgggtgcgct
     3661 gatgggtgca gaggcgctga tccgctggaa tcatcctacc cggggtcggc tgctgccggc
     3721 cgaattcctg ccagtgatcg agagccatcc gctgatcgat cgcattgggg agtgggtgat
     3781 cgatcatgcg ctcacgcaac tggagcgctg gaatgctgcc gggctgaaga ttccggtgag
     3841 cgtgaacatt gccgcgcacc atctgcagca accggatttc acggatcggc tgcgggcgct
     3901 gctcgccgag catgcggaga tttcgccttc gcggctggag ctggaggtgc tcgagaccag
     3961 tgcactgcgg gatgttgcgc aggtctcggg ggtgctggcg gcatgccgcg atctgggagt
     4021 ctcgattgcg ctcgatgact tcggcacagg gtattcgtcg ctgacgtact tcaagcggct
     4081 gccggtgaat acgctgaaaa tcgaccgcag ctttgtgcgg gatgtgctcg acgatccgga
     4141 ggacctgagc attctggaag gggttctggg gctggcaacg gctttcgatc gcatggccat
     4201 tgcagaaggc gtggaaacgg ccgaccacgg acggctgctg ctgcagctgg gttgcgtcta
     4261 tggacagggg aacgggattg cgctgccgat gccgggggat gaattgccgg gatggtcatc
     4321 gacatggaat cccgacccga tgtgggtcaa cgtggcctcg gtcgcgccgc gcaaacggag
     4381 cctgatgcac gccgctgtgg ctcatcgcgc gtgggtcgcc ggcctggaag gttttctgca
     4441 ggggacgcgg cgcacaccgc cgaccctgga tccacatcga tgccggctgg gccgctggct
     4501 ggacagcgaa cgcgaccatc ccatgggaag cagcgcggcg tttcggcgga tcgaacaact
     4561 ccaccaggaa acgcacgcct tcgccgatgt tgcatcggca tgggaaccgt cggccacggg
     4621 agcggatgtt tcgactcagg tgaaggagct gcatcggctg cgcgatgcgc tgatcgagca
     4681 gatgaacagc ctgctgcagg atacggctgc gcagtgattc gcgaggtccg cgtgcgcttg
     4741 tccgggccgc gggatcaggc gacgcgattc ttgaggacag atagaatctc tcccgcctcg
     4801 gcgatgttga aggaaggccg ggagggcgcg gatttctcac tcgagggagc gagggcagcg
     4861 gcgtttgatt tgtccggaat ccacaggccg gcttcttttt tggcgggaac gatgttgccg
     4921 ccgaaagtga cggcgtactc gccgtcggga agatcgtgag gggcgtcgat cacgctgcag
     4981 cgcgaataaa cagcgggccc tgccttctcc ggggtttccc gaatggcgag catctcgcag
     5041 ggctcctgac gcccattgcc ctggagcagt ccgtggatca gcacgaccga gcgcattctc
     5101 aattccctgc ccctgctacg tgtgaatcga aacgctgtga cccgggatgc cccgcgattg
     5161 gtgcagagtt gccgaagact gaaggttttt tcatgaagat gcgttactgc gggcatctgc
     5221 cggcgtcgaa agcatggcgt cgcctaccat ggaagggtgg ctggctttcg tgaacggctg
     5281 gggcaggtag aggagaagat tggagcagcc tgtcatcgtg cgggccgccc gcgcgaagag
     5341 gtacggctga tgggcgtctc caagatgcat ccggctgagg cgctggcgga ggctgcaggc
     5401 gctgggctta tattgtttgg cgagaaccgc gtgcaggaat tcgaggccaa gcggctgcgg
     5461 atcgcggagc tggggcttga aggactggag gtccacctga tcggccatct gcaggcgaac
     5521 aagacgggaa aggccgcaga gatgttcgac ggggtggaca ctgtcgattc gctgcgcgtg
     5581 gcggagcgac tgaacgacgc cgcgggcaag ctggggaaga gcctgcccgt gctggtggag
     5641 gtaaagctga gcgatgagcc gagcaaagcg ggggttgcgc cggaagatcc cgaactgagc
     5701 gtgatgctgg agcgcatgcc ggagctttct catttggcga tgcggggtct gatgacgatt
     5761 gcgccgctgg atgaaaatcc ggagacggcc cgggcttgct tcaggcggct gaggacgctg
     5821 cgggacgaac tggcgagaaa gtttggccgc gtcaatttcg acgagctttc gatggggatg
     5881 tcaggggatt ttgaaattgc gatcgaagaa ggctcgacgc tggtgcgaat tggaacggcg
     5941 ctgtttggag cgcgtgcgct gccatgactt cccgtgtgcc ggtacgagag acagcgcagg
     6001 gagcgagttt tctggtgcgt gtgatgcccc gcgcgagccg cacagccatc gtgggcgtgc
     6061 tgggtgacgg cacggatgcg gccgtgaagg tcgcgctgca ggcgccgccg gtggaagggc
     6121 gagcgaatgc ggcgctgatt gagtttctgg cggagttgct tggggtggct cgctcggcaa
     6181 tcgagatagc aggcggcgaa accgcgagaa cgaagcgcat cgtggtccgg ggaaaggacg
     6241 cgatggaggt agctacagcg attgccatcg cgctgacgca ctagggtggc gctttttctg
     6301 agccggcgcc ggattcgcgc gctatccggc ctttttcttc tttgccgcgg tttttgatgc
     6361 gactcgttgt ggcggcttct tgtctttgtc ggggtcgggc tgcggagcag cagaggctgt
     6421 gaccgatgcc ggactggcct gacccatgct cttgcgcagc gcttccatga ggtcgatgac
     6481 cggggcgagc ttcttcttcg gctgcttgcc gggtgcagga gcctggccct gcttgctctc
     6541 gatgagctgc tcgacgcgag ccgtgtactc gtctttcatc ttctcaggat tgaaggcggc
     6601 ggtgagctgc tgcatgaact gctcggcgag cgcgacctcg gccttgttgg tctcgacttc
     6661 gggctggttg tcgtattcgg cgacggcgcg aatttcttcc gggtagtaga gcgtgtgcag
     6721 gaccagaccc ttctcgaagg gacgaatcat gacgatctgc tcgcgctggt ggagggtgat
     6781 tttggcgatg gccccgagct tgagatcctg catacccttg tggatcagcg aataggcttt
     6841 gcggcctgga tcttcggcga cgctgaagta ggaagtctga tagtagagcg ggtcgacctc
     6901 ggggaggctg acgaactgca cgatctccat agtctcggag gactgggctt cgaggccttt
     6961 gagttcttcg ctggagacga tgaggtagtt ttccttgtcg acgggatagc ctttgacgag
     7021 ttcgctgcgc tcgacgagcc gctcgcaggt ggggcagaag gtctgctgct tgatgcgcga
     7081 gccgcacacc tggtgaatct ggttgaagct gatgtggttt tcgcgagccg cgacgaagag
     7141 gcggatcggc accgagatca gaccgaacga gatgtagccc ttccagacgg ttgaagccat
     7201 agggaaccaa agaatatcgc accaaaaatg gggcgagaaa tgaaaagtga tgaaggaatc
     7261 ggggggatta ctgccccttt cgccttgttt tcgcctgcgc gggttgggca ttgcattcga
     7321 ggcggcggct gaacaaggtg cagaagagat acagccagtg gagagagacc tcaggggcta
     7381 aagcccggat ccttatgggg gctgaccggc acgactaaaa gtcgtgccct gatacaaagt
     7441 cgactttttc gcgagccttg aagcgagtcg atacaacagc tgccttgatc cgcccccctt
     7501 cacaacggcc tactttgctc ttggatgcgt gcggtcgtag acctcggcga cctggccgac
     7561 ggtgagctgg gtgtatcgct gggtggtcga caggcgctca tggccaagca gttcctggat
     7621 ggcgcgcagg tcggcgcctt cttcgagcat gtgagtgccg aaggcgtggc gcagggtgtg
     7681 cgggtggacg tcggcgggga gtccgtgggc gagcgcgatg tgcttgacga tgcggccgac
     7741 gctgcgcgtg gtgaggcggg cgtcgccgcg gaggcgcaga ttcagtaaca gtgccggtga
     7801 cgatttctgt gcggcagcga gtttttgctc gcgcagagga aggtaggcgc ggagggcctg
     7861 ggctgcggcg tcgccgagag ggacgtagcg ctccttgcgt cctttgccgc ggacgaggat
     7921 agcgtcgttg gcccagtgaa tgtcggcgag gtcgatgccg acgagttcgg cgttgcggat
     7981 gccgcagccg tacagcaact cgaaaatcac gcgatcgcgc tcgggccagg cggcggcgtc
     8041 atcggcggcg ctttcgacga cgcggttcat gtcttcgatg gtggggacgc ggggcaggtg
     8101 cttcggcagt ttcggcgtag agacgagggc ggcggggctc tgctcgacga ggccgtggcg
     8161 ggcgagccat ttgaaccatg agcggatggc ggcgagggcg cgggcgacgg aggctttgga
     8221 gagaccgcgg ccgtagagtt cgctgagata cgcgcggatg tgctggtgct cgatttgctt
     8281 cggcgcgatg ccggcgccga gtttttcgac gatgaaggcg gcgaagttat ggagttcgcg
     8341 atcgtaggcg cgcgcggtgt gggggctggc gttgcgctcg tgggcgagca tggcgaggaa
     8401 atcgtcgacg tggcgggcaa cttcgggttt gggcgcaggt ttgcgcgttg ctttggccgc
     8461 ggtcatgatg taacccgctt cgcgcgcgga tgatgttccc ggtggacagc tttgaggcgg
     8521 cccagagcaa ggtgcgtata gacctgcgtg gtggcgatgt ccgcgtggcc gaggagggtc
     8581 tggacggtgc ggagatcggc tccgcgttcg accatgtggg tggcgcaact gtggcggagc
     8641 atgtgcgggc tggccgtttg ctcactgctt ttgaccagat gccagaccca ggcacggctg
     8701 aggggcagcc cgcgggtgga gaggaagagg cggccggaag atttcttctc gagtgcgggg
     8761 cggccgtgtt gcagatagcg ttcgagcgct tcgatggagg cgcggccgag agggacgatg
     8821 cgctccttgt cgcctttgcc gcgaacgagg gcgcggccgg catcgagcga gaggtcggcg
     8881 acattgaggc ccgtgagttc ggaaacacgg agaccggcgg cgtagagcaa ttcgaggatc
     8941 gctcggtcgc gaagagcgat ggcatcggcc tgagggtgat cggcagcgag agcggcgcgc
     9001 tccatcatgg agtcgacttc agagacagcg agcgatttgg ggaggacctt ccaggtcgaa
     9061 ggcgattcga ggttgagggt ggggtcgcgg cggatgcgct tgtcgaggag gagccagcgg
     9121 aagaatccgc gcaggcagga gagcttgcgt gcagcggaac gcgattcgac gctgtgtttg
     9181 gagaggtgcg cgaggaatcc ggcaacttgt tgctgtgagg cggcgaggag ggtgacggat
     9241 tcgctttcga ggaattcggc gaactgcagg agatcgcgct cataggcttc gcaggagagc
     9301 gggcggagac ctttttcgac gcgcagatag gcgaggtatt cgcggatgag ggcggagttg
     9361 gtcgaattgg gcgtgctcgc ttgccggttc gcttttgggg gagcgctcac gtgttcatta
     9421 tcgaagaggc ggcgggctgg gatgagtatc gagggtggtt caggtcctgg ccggacgggc
     9481 agacgcgctt cgcgccatca cgattcgcgg tagttaagaa ggaggatgcc gacgaggatg
     9541 aggcaggcgg gccagatcca cgcggcgatg cggcgttcgc gagagggcgg atcggcgagc
     9601 gtggcgttgt cgaggcgaag ttcgagccag cggctccatc cggcgaccgc gccgagcacg
     9661 gccatgggcg tgtggctgag ttcggcgaag acttcgtcgt tgccttcgcc gagggcgtgc
     9721 gtgtgcgtga ggaggagcgc accgccgaca gcacacagca tggggaagac ggagcgggcg
     9781 gcggacgagc ggaagcggcc ggtctggacg ccggtctcaa agacggcgaa gacagcgatg
     9841 atgagggcgc agagacgatg ctgaaggacg tcgggggcag tgaagctggc ccagaaggga
     9901 cgtgggccga gcggccagca ctcagggtcg gcgcgcagga ggatgaagat ggcgaggcct
     9961 aagaagagca gcggccagta gcgtgcccag gtttgtcgag gcaaacgcga gaccagcgcg
    10021 agcaggccgg cgatgagcac gatcagtccg gcccagtggt ggttgtactc cgaccaggcg
    10081 cggtcgttgg cgtcgctggc gctgccgccg gtataggtgg cttcgcgcac ggcaacactg
    10141 agcggcgtgg gcggtgcgag ttgcgcgagg gcgggcgtgt ggaagctggg ccagcgccat
    10201 tccatgcggt caacgacctg atgggccgtg agggtgtcct gaggggtttc gtcgaccgag
    10261 ggggactgcg cggtcagcga agcggcggcg agaatgacgg tgaagccgag gccgatttcg
    10321 gtctcgccga agcggcgaag gcggaagagg agc
//
LOCUS       DASZYN010000416         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_20236, whole genome shotgun sequence.
ACCESSION   DASZYN010000416 DASZYN010000000
VERSION     DASZYN010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20236"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VHE33_10785"
     CDS             <1..323
                     /locus_tag="VHE33_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97983.1"
                     /translation="ALDALLKEGEYLPLALTFLSTQLRLGLAAQEAGLKSASQIQSYF
                     TQQGIRIWRDRAEQIAQTVAAFPRRRLEMAIQRIYQADKALRDTRPDDRVVMEEFVLG
                     VTAR"
     gene            complement(338..607)
                     /gene="rpsT"
                     /locus_tag="VHE33_10790"
     CDS             complement(338..607)
                     /gene="rpsT"
                     /locus_tag="VHE33_10790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HVT97984.1"
                     /translation="MANTFSSLKRVRVEARREEVNRNRKSRLRTQVRAMRRLLDGKDA
                     SGAALALPQTFSLIDRSARWGIIKKNTAARYKSRISHRIKAISAA"
     gene            731..806
                     /locus_tag="VHE33_10795"
     tRNA            731..806
                     /locus_tag="VHE33_10795"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:764..766,aa:Ala,seq:cgc)
     gene            complement(892..2070)
                     /locus_tag="VHE33_10800"
     CDS             complement(892..2070)
                     /locus_tag="VHE33_10800"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HVT97985.1"
                     /translation="MTDSEILIGLPGYQITGIEQEKGEVRISARRTEPEPCPHCGGTR
                     LRSKGRYQRRVRHEDWGLRRCVLLLEGHKFQCRDCARSFRERFPGILPCQRASEAFRQ
                     WIFQQHLDGINRSRLGRREGIGAATVERHFRHGLDRQFRQWHSRCCPRVLGIDEHFFT
                     RKKGYATTLCDLGNHKVYDVVPGRSEASLERYLLSLEGKDLVRVVCMDLASVYRALVR
                     KHFPQARIVADRFHVIRLVNHHFLNCWREIDPVGSKNRGLLSLMRRHRHNLRPEQQAR
                     LMAYLEKYPALDAIYRFKQRLCYLLLKKHRTQRQCQALIPRFLRSVAQLRQAGLPQLV
                     QLGETLAAWSEEIVAMWRFTKNNGITEGFHNKMELINRQAYGFRNFENYRLRVKVLCG
                     "
BASE COUNT          454 a          682 c          701 g          440 t
ORIGIN      
        1 aagcactcga cgctttgctg aaggaaggcg agtatctgcc gctcgcgctt acttttctgt
       61 ccacgcaact gaggctcggt ctggcggcgc aggaggcggg actgaaaagc gcatcgcaga
      121 tacagtccta tttcacgcag cagggaatcc gaatctggag ggaccgggcg gaacagattg
      181 cgcaaacagt agccgcattc ccacgccggc ggctggaaat ggcgatccag aggatttatc
      241 aggcggacaa ggcgcttcgc gacacacgcc cggatgaccg ggtggtgatg gaagagttcg
      301 ttctgggagt aacggcgagg taggctcccg ccacgcctta ggcggcggag atcgctttga
      361 tgcggtggct gatgcggctc ttgtagcgcg ccgccgtgtt cttcttgatg attccccagc
      421 gggccgagcg atcgatcagc gagaacgtct gaggaagagc caaagccgcg ccgctggcgt
      481 cctttccatc gagcaggcgc cgcatggcgc gcacttgcgt gcgaagacgg gacttacgat
      541 tgcgattgac ctcttcgcgc cgggcctcga cgcggaccct cttcagggat gagaaagtat
      601 ttgccatcgg ttaactgggt tccttacaga gacgtacagc atgtaggata ccacgaatcg
      661 aatccgtctg tggcggcccg gcgaaaattg aattagactg taaacagcag tagtttttcc
      721 ttcctggagt gggcggctag ctcagttggg agagcacggc gttcgcaacg ccggggtcgt
      781 gggttcgaat cccatgccgt ccaccaaaaa caggctctac gccaacaacg ggggcacggg
      841 gagtgcccgg ctctacgcca acaacggggg cacggggagt gcccgagaag atcaaccaca
      901 taataccttg accctgagcc tgtagttttc aaagttgcga aacccatacg cttgccggtt
      961 gatgagttcc attttgttgt ggaacccctc ggtgatgccg ttgtttttcg tgaagcgcca
     1021 catcgcgacg atctcttcgg accaagccgc cagggtctcg ccgagctgca ccagttgcgg
     1081 caagcccgcc tgacgaagct gcgcgaccga acgcagaaag cgcgggatca gagcctgaca
     1141 ctgccgctgc gtccgatgct tcttcaacag caggtaacac agacgctgct tgaagcgata
     1201 aatcgcatcg agcgccggat acttctccag gtaagccatg agccgagctt gctgctccgg
     1261 ccgcaggttg tgccggtgcc gccgcatcag cgagagcaga ccgcgattct tcgaacccac
     1321 cggatcgatc tcgcgccagc agtttaggaa gtgatgattg acgaggcgga tcacgtggaa
     1381 gcggtcggcc acaatgcgag cttgcgggaa gtgctttctc accagcgcgc gatacaccga
     1441 ggccagatcc atgcacacca cgcgcaccag gtccttgcct tctaaggaaa gaaggtagcg
     1501 ctccagcgat gcctcgctgc ggccgggcac gacgtcgtaa accttgtgat tgccgagatc
     1561 gcagagtgta gtcgcgtaac ccttcttgcg agtgaagaag tgttcgtcga tgcccagcac
     1621 gcgcgggcaa cagcgagaat gccactggcg aaactggcgg tcgaggccgt gccggaagtg
     1681 gcgctcgaca gtggccgccc cgatgccttc cctgcggccc aagcggctgc ggttgatccc
     1741 gtcgaggtgt tgctggaaaa tccattggcg gaaggcttcg ctggcgcgct ggcagggcag
     1801 gatgccggga aagcgttcgc ggaaggagcg cgcgcagtcc cggcactgga acttgtgtcc
     1861 ttccagcagc agcacgcagc gccgcagtcc ccagtcttcg tgcctcacac ggcgctggta
     1921 gcgccccttg ctgcggagcc gtgtcccgcc gcaatgcgga cacggctccg gctcggtgcg
     1981 ccgcgctgag atccgcactt cccccttttc ttgttcaatg ccggtaatct ggtagccggg
     2041 cagtccaatc agaatctccg agtcggtcaa agctttctcc caagttggtt ttatcaactc
     2101 gggttcccgg cctgcccccg tttctgatca agagccaaaa tccggggctc taaatcggtc
     2161 ggcacgcttt ggatccaggt ccgtcaaacc tcatcgcccg gtaatggatg tcgtaccgct
     2221 ccgtgagcgg ccgctcacgc actcgtcgat tcccgccaca acgtcccgca gtatttg
//
LOCUS       DASZYN010000417         2538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_148643, whole genome shotgun sequence.
ACCESSION   DASZYN010000417 DASZYN010000000
VERSION     DASZYN010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148643"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /locus_tag="VHE33_10805"
     CDS             complement(<1..1535)
                     /locus_tag="VHE33_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97986.1"
                     /translation="MNTVRRFRFVAVMLLGLASIPCFAKTVVFWQAGFPVADSPAPEE
                     AGLRAGFAGAEFVGAAGLGLALGRSDTDLLVLPYGSAWPEAEWGAILGYLDRGGNLIV
                     LGGKPFTRAAFEDGSGWHLRTASVAQSLELFIHDYQETLGTSSDVKFAANPDVFPQLP
                     AFGWKRAFSPVVRLSVVDGDTTGGHTGYEDMDLTTLVWGEREGHKLTAPVVELDHNTH
                     RFVGGRWILVACEPDAEFFRNTKLLAMLGEIAVRKQDRFTYRPRLPLFVPGDAMEFRM
                     KPSDPMAEPPVGDQLRVKISDEDGTVLYDQTAALPGAHRLTLRANAEDGRGLHTVEAT
                     LIRDGKPLRVYRSGYWLRDWDYLLSGPKLTAGADYFRLNGKPLPVVGTTYMSGDVSRM
                     YLIQPNAYVWDQDMTQIHDAGLNMIRTGLWTTWKQLLAPNGQMSEDGLRTIEAFLMCA
                     RHNNLPVQFNLFAFYPDEFGGVHGYLDPAALQAESRYAESVVARFSDVPFLAWDLINE
                     PSA"
     gene            1735..2187
                     /locus_tag="VHE33_10810"
     CDS             1735..2187
                     /locus_tag="VHE33_10810"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HVT97987.1"
                     /translation="MRRAIALASENVRTGRGGPFGAVIVKSGAIIGEGSNTVTRDNDP
                     TAHAEVSAIRAACLSLGVFTLKGCEIYSSCEPCPMCLSAIYWARVDRIWYGNTSADAA
                     RIAFDDAFLYREVALPARDRAIPSSMFLRDQAWESFRLWLDTPNKTPY"
     gene            2270..>2538
                     /locus_tag="VHE33_10815"
     CDS             2270..>2538
                     /locus_tag="VHE33_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVT97988.1"
                     /translation="MLGRSGLKVSVLSFGAATFGGGNEFFKAWGSTQVDEARRMVDLC
                     LDAGINLFDTADGYSNGVSEEILGKAIQGKRNKLLISTKTFFPLS"
BASE COUNT          521 a          931 c          626 g          460 t
ORIGIN      
        1 ttcgcgctcg gctcgttgat caggtcccag gccagaaacg gcacatcgct gaaccgagcc
       61 accaccgact ccgcatagcg cgactccgcc tgcaacgccg ccggatcgag atacccatgc
      121 accccgccaa actcatccgg ataaaacgca aacagattga actgcaccgg gagattgttg
      181 tggcgcgcac acatcaggaa cgcctcaatc gtccgcaaac cgtcttcgct catctgccca
      241 ttgggcgcca gcagttgctt ccacgtcgtc cacagtccgg tccggatcat gttcagcccg
      301 gcgtcgtgaa tctgcgtcat gtcctggtcc catacgtatg cattcggctg gatcagatac
      361 atgcgtgaaa cgtcgccgga catataggtt gtgccgacca ccggcagggg cttgccgttc
      421 agccggaaat agtccgcgcc cgccgtgagc ttcggtcctg agagcagata gtcccagtcc
      481 cgcaaccagt agcccgaacg ataaacccgc aacggcttgc cgtcgcggat cagtgtcgcc
      541 tccaccgtgt gcagcccccg cccatcctcc gcattcgccc gcagcgtcag ccggtgcgct
      601 ccaggaagcg ccgccgtctg gtcgtacaac accgtcccgt cttcatcgct gatcttcacg
      661 cgcagctgat cgcccaccgg cggctccgcc attggatccg acggcttcat tcggaactcc
      721 atcgcatcgc ccggcacaaa cagcggcaac cgcggccgat acgtgaagcg atcctgcttc
      781 cgcacagcga tctctcccag catcgccaac agcttcgtgt tcctgaagaa ctccgcatcc
      841 ggctcgcacg cgacaagaat ccaccgtccg cccacaaacc gatgcgtgtt gtgatccaat
      901 tccacaaccg gcgcagtcag cttgtgaccc tcccgctcgc cccacaccag agtggtcagg
      961 tccatgtcct catagccggt gtgaccgccg gtcgtatcgc catccaccac gctcaggcgc
     1021 accactggac taaatgcccg cttccaccca aacgctggaa gctgcggaaa cacatccgga
     1081 ttggctgcaa atttcacatc cgaagacgtc cccagcgtct cctgataatc gtgaatgaac
     1141 aactccagcg actgggccac gctcgccgtc ctgagatgcc agccgctacc atcctcaaac
     1201 gccgcgcgtg tgaacggctt accccccagg acaatcagat tccccccacg atccaaatat
     1261 ccaaggatcg ccccccactc cgcctccggc cacgccgacc catacggcag caccaacaga
     1321 tccgtgtcac tgcggcccaa tgccaaaccc agcccggcag cacccacaaa ctccgcgccc
     1381 gcgaagcccg cgcgcaaccc agcttcctcc ggagccggcg agtcggctac cggaaaaccc
     1441 gcttgccaaa agaccaccgt cttcgcaaag cagggaatag aagccaagcc aaggagcatg
     1501 accgcgacaa aacgaaatct gcgaaccgtg ttcatcacgc gctccaccat acacccggtt
     1561 cccccaaagg aaacgtgaga aagcgcatcc cccacctcgg cagccggagg cccatatctg
     1621 cccggtttcg gcagatgtgg gcgggcacga tacctcgctc ggcggtacac cccctgtgcc
     1681 atactgattt cggtttacac cggcacaatg catccttccc cgaatccgga cttcatgcgc
     1741 cgggcgattg ctctcgcctc ggagaatgtc cgcaccggac gcggcggtcc cttcggtgcc
     1801 gtgattgtca aaagcggcgc catcatcggt gaaggctcga acaccgtcac ccgcgacaac
     1861 gacccgacgg cccacgccga agtcagcgcc atccgcgccg cgtgcctgtc tctcggagtc
     1921 ttcacactga agggctgcga aatctactcc agctgcgagc cctgccccat gtgcctctcg
     1981 gccatttatt gggctcgcgt cgaccgcatc tggtacggca acaccagcgc cgatgcggct
     2041 cgcatcgcct tcgacgacgc cttcctttat cgcgaagtcg cgctgcccgc ccgtgatcgc
     2101 gccatcccct catcgatgtt cctgcgcgat caggcctggg aatccttccg gctttggctc
     2161 gacaccccca acaaaactcc gtattaattc agagccctgt gctctgagac ctgtgccctt
     2221 gcaccatcca caagttctcc cgcatcctta tgtccatatg gaataccgtt tgcttggtcg
     2281 ctccggactc aaagtctccg ttctcagctt cggtgccgca accttcggcg gcggcaacga
     2341 gttcttcaaa gcctggggtt ccacccaggt cgatgaagcc cgccgcatgg tcgacctctg
     2401 tctcgacgcc ggtatcaacc tcttcgatac ggccgacggc tactcgaatg gcgtttccga
     2461 agagatcctc ggcaaagcga ttcagggcaa gcgcaacaag cttctcatct ccaccaagac
     2521 attctttcct ctgagcga
//
LOCUS       DASZYN010000418         7397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_20541, whole genome shotgun sequence.
ACCESSION   DASZYN010000418 DASZYN010000000
VERSION     DASZYN010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20541"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1572
                     /locus_tag="VHE33_10820"
     CDS             172..1572
                     /locus_tag="VHE33_10820"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HVT97989.1"
                     /translation="MSASTPVTLLPATEQIALLRRRGITPRELAEEHIAQIRRLNPQI
                     NAVVDFEADRIRKQSARVRPGPLSGLPVTVKSSIAVAGYRCEIGSTINRGSIPEKNAV
                     LVDRLIAAGAVILGTTNCPEFLMAYETENRLYGSTRNPWNLEFTAGGSSGGESAAIAA
                     GMSAGGLGSDSGGSVREPAHFTGICALKPTAGRFPAGGHLPPCLGPFAFLGSIGPMAR
                     TIGDVRLLFETLSGQDPLDPNSAPVPQRPVSRDQAKQIPIGYFEDDGLVPVSAETRTA
                     IRQAAQALQDQGFTVKPFRPASLEAARQLWFTFFVQCGAMLYAPTIRGREQELSPTFR
                     DFLTIARAQSPLTADTLLNAWVESDKVRSRLLEEMRACPILLCPVCSVPAFKPFERSW
                     TIDGQEVSYLDAMRFTQWFNLLGSPAAVVPVTRSAEGLPIGVQIAGRPYEDELVLEVA
                     QAVESAFGYQPPPIAL"
     gene            complement(1654..3030)
                     /gene="rseP"
                     /locus_tag="VHE33_10825"
     CDS             complement(1654..3030)
                     /gene="rseP"
                     /locus_tag="VHE33_10825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HVT97990.1"
                     /translation="MPFAVTATISFLIVLGIMVLVHEFGHFAVAKLCGVRVEVFSLGF
                     GTRLFGVKYGDTDYRISALPLGGYVKMSGETPGSETTGDPGEFSAHPRWQRVLIAVAG
                     PVANFVLAFILMAGLYTMHNEVEQYLAAPAVLDVVPQNSPAGRAGLQTGDRIIHFDDK
                     KNPTWETLTDRAMLDANTTVPITVERTVNGQTKQFSTALLLADPSHGEDFDIESLGLL
                     PKEQDGPVVVADVMPGNPAAKAGLQAGDGLIAVDGQEVHSVAAIIAILDGAGSKPADL
                     LVDRNGKQFTVTVQPIWADYGSGEPGYRLGFTAVPPPFTVEQLPVPKAIQRSISYNLH
                     YSGLILDILHRMFTRHGQVMQQLSGPIGMARMTGEAAEMHGWQPIIGLTALISLNLGI
                     MNLLPIPILDGGMILLLCVEGLLRRDLKQEFKERIYQVAFVMLILFFAIIMVNDVSKL
                     NLFQHLKP"
     gene            complement(3098..4273)
                     /locus_tag="VHE33_10830"
     CDS             complement(3098..4273)
                     /locus_tag="VHE33_10830"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF009114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HVT97991.1"
                     /translation="MKQIAILGSTGSIGTSTLDIVARYPERFTVASLAAGRNVDAAFE
                     QCVRWRPRVVSLADEELAGDLAARLRAAGVDTEVVWGSAGTVRVATLKEVDFVVSAIV
                     GVAGLEATHAAVQAGKTIGLANKEAMVAAGEILTAAARAKKVALLPIDSEHNAIHQCM
                     RAGERREVRQIWLTASGGPFRTLPASEFSRITVEQALHHPTWVMGKRITIDSATLLNK
                     GLEIIEACRLFDLPPSQVQVTVHPQSTVHSLVEFVDGSLLAQISVTDMRLPILYALSY
                     PERVESELRFDVAALSDLHFEPPDMERFPCLRLAYEAAEKGGAHPIALNAADEVAVEA
                     FLEGVIPFTGIPDTIEQVLQTTPETHPATIEEVLALDRQAREQAQEVLAGRGAARRC"
     gene            complement(4344..5174)
                     /locus_tag="VHE33_10835"
     CDS             complement(4344..5174)
                     /locus_tag="VHE33_10835"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HVT97992.1"
                     /translation="MKRILTAVVLIPLVIAVIYVSPRWPIVLMGALFVIAALALWEYL
                     GLADAIGAKTPKIVVTVLLAVQMAAIFRNPELMAPISGALALLMLVICTFRSPLERVL
                     PDTAFSVFGLLYIGLPLSMMYLLSAQDNGPSLLVFLLFVVWAGDIAALYVGRSMGRWT
                     LAKRLSPNKTWEGSIASAVASIGVAVLLVWLGGTLAARSIMYLSYPGTYAHWIVLAVL
                     LNIAGQVGDLAESAIKRAAGAKDSGKLLPGHGGFLDRIDALLLAAPVLWYAQLAQQLF
                     "
     gene            complement(5507..6784)
                     /locus_tag="VHE33_10840"
     CDS             complement(5507..6784)
                     /locus_tag="VHE33_10840"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HVT97993.1"
                     /translation="MKGRIAWVGVAGLLLVAPLAFAFSFLTHETIIDITWVNGIKPVL
                     LAKYPNTTEKELETAHAFAYGGCVIQDAGYYPFGHPPFSNLAHYVRTGDFITNLIHQA
                     HNVNELAFALGALSHYVGDSIGHREAVNPSVAIEFPDLEKEYGPVVTYEQDPHAHIRT
                     EWAFDIDQLSHARFAPAVYLRNVGFQVPVKLLERAFYATYGLRLNKIVGHERHAIASY
                     DWAVRQFLPRISYAEVLLHRKSFPPDQDLPGFHTFAERVKKASVDNGWEQYRKHKVSF
                     KTRIVAFLILITPKIGILSDLAIRGPSRESEENYVEAVNATIDRYQQLLGELAQKGND
                     GFAVPDLDLDTGYPTRPGTYRLTDKTYATLLHEVTEQNAMPPLGLRQNILAYYSNPNA
                     PIETKKHPKKWAKVQKELEVLRSMPATRIPEAK"
     gene            complement(6858..7133)
                     /locus_tag="VHE33_10845"
     CDS             complement(6858..7133)
                     /locus_tag="VHE33_10845"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HVT97994.1"
                     /translation="MQIRWTPNATDDLERIAAYLKEHHPGYAESTVRELNANVQSLQK
                     FPRLGRLGREEGTRELVFGRLPYVAVYRVKDEAVEVLRIWHAAQNRL"
     gene            complement(7137..>7397)
                     /locus_tag="VHE33_10850"
     CDS             complement(7137..>7397)
                     /locus_tag="VHE33_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT97995.1"
                     /translation="SGGGAGSSYNGAMEVRLTPDEEARLAAIAAVVGTDPEQLIKDAA
                     LNLLDEDQRFREAVRAGIAQADRGEFIEQAEINARIDRMFPS"
BASE COUNT         1434 a         2575 c         1983 g         1405 t
ORIGIN      
        1 cgggcatggc ctcatcctag cattcggccc tgccgctgcg atgactcgtt ctggggatga
       61 tgaagcaggt tcccgccgaa tacgccgatc gcttttgctg accgtcttac cgctgaccgc
      121 ttcacggctg accgtctcac ctgaatttat aatttgcctt caccttcatt catgtctgcc
      181 tcgactccgg ttaccctcct ccccgccacc gaacagatcg cgctgctgcg gcgccgtggt
      241 atcacaccac gcgagctcgc cgaagagcac atcgctcaaa tcagacgcct gaatccgcag
      301 attaacgccg tggtcgactt tgaggccgac cgtatccgaa aacagtccgc gcgcgttcgt
      361 cctgggcccc tttccggcct tcccgtcacc gtgaagtctt cgatcgcggt tgccggctat
      421 cggtgcgaaa tcggcagcac gatcaatcgc ggttcgattc ctgagaaaaa tgccgtgctc
      481 gtcgaccgat tgattgccgc cggtgcggtc attctcggca ccaccaactg tcccgaattc
      541 ctcatggcct acgagaccga gaaccggctc tatggcagca ctcgcaatcc ctggaacctc
      601 gaattcacgg caggaggctc cagcggcggc gagtcggcgg ccatcgcagc aggcatgtcc
      661 gcgggaggtc tgggcagcga cagcggcgga tccgttcgcg agcccgccca cttcaccggt
      721 atttgcgcac tgaaacccac agcgggccgc ttccctgctg gcggacatct gccgccctgc
      781 ctcggaccct tcgccttcct cggctccatc ggccctatgg cgcgcaccat cggcgatgtg
      841 cggctgctct tcgagacgct ctccggccag gatcccctcg accccaacag tgctcccgtt
      901 ccacagcggc ccgtctcgcg cgatcaggca aagcagatcc ccatcggcta cttcgaagac
      961 gacggcctcg tccccgtcag tgcggaaacc cgcacggcca ttcgccaggc ggcgcaggct
     1021 ctccaggacc agggcttcac ggtgaaaccc tttcgccccg cctcgctcga agccgcccgc
     1081 cagctctggt tcaccttttt tgttcagtgc ggcgcgatgc tctatgcacc cactatccgc
     1141 ggccgcgagc aggaactcag cccaaccttc cgcgacttcc tcaccatcgc ccgcgcacaa
     1201 tcgcccctta ctgccgacac gctgctcaat gcctgggtcg agtccgataa agtccgctcc
     1261 cggcttctcg aggaaatgcg cgcctgtccc atcctcctct gccccgtctg ctccgtgccc
     1321 gccttcaaac ccttcgagcg cagctggacg atcgacggcc aggaggtctc ctacctcgat
     1381 gccatgcgct tcacgcagtg gttcaatctg ctgggatcgc ctgccgccgt tgtcccggtg
     1441 acgcgctcgg ccgaaggcct tccgatcggc gtgcagatcg ccggccgacc ttatgaggac
     1501 gaactcgttc tcgaagtcgc ccaagccgtc gaaagcgcct tcggctatca gccaccgccg
     1561 atagcgctgt aaactcccgc tcttctccgc cacagacgag aaaaggtccg ccaatcggcg
     1621 gaccttctgt gccctgtgac ctgagctctg cctttacggc ttcaaatgct ggaacagatt
     1681 cagcttcgac acatcgttca ccatgatgat ggcgaagaac aaaatcagca tcacgaacgc
     1741 gacctggtag atgcgctcct tgaactcctg cttcagatcc cgccgcagca acccttccac
     1801 gcacagcagc agaatcatgc cgccatccag aatcggaatg ggcagcaggt tcatgatgcc
     1861 gaggttcagg ctgatcaacg ctgtgagccc gatgatcggc tgccaaccgt gcatttcggc
     1921 ggcttcgccc gtcatgcgcg ccatcccgat cggcccgctc agttgctgca tcacctggcc
     1981 gtgccgcgtg aacatccgat gcagaatgtc gagaatcaat cccgagtagt gcaggttgta
     2041 gctgatcgat cgctggatcg ccttcggcac cggcagttgc tccaccgtga agggcggcgg
     2101 aaccgctgtg aaaccgagcc gataaccagg ctcacccgac ccgtaatcgg cccagatcgg
     2161 ctgcaccgtt accgtgaatt gctttccatt gcgatccacc agcagatcag ctggcttgct
     2221 gcccgcgcca tccagaatcg cgataatcgc tgcaacgctg tgcacttcct gcccatccac
     2281 cgcgatgagt ccatcgccgg cctgcagtcc cgcttttgcc gccggattgc cgggcatcac
     2341 atcggccacg accaccggcc cgtcctgctc ctttggcagt aaccccagcg actcgatatc
     2401 gaaatcttcg ccgtgcgaag gatcggccag cagcagcgcc gtactgaact gcttcgtctg
     2461 cccgttcacc gttctctcca cggtgatcgg caccgtggtg ttggcatcca gcatcgcccg
     2521 atcggtcagc gtttcccacg tcggattctt cttgtcgtcg aagtgaatga tgcggtcgcc
     2581 cgtctgcagg cccgcgcgcc cggccggcga attctgcggc actacgtcga gaacagccgg
     2641 ggccgccaga tactgctcca cctcgttgtg catcgtgtac aggccggcca tgaggataaa
     2701 cgccaaaaca aagttggcca ccggcccggc gacggcgatc aaaacccgct gccagcgcgg
     2761 atgcgcgctg aactcgcctg gatcccccgt ggtctccgag cccggcgtct cgcccgacat
     2821 cttcacgtat ccgcccaggg gtagcgcgct gatccggtag tcggtgtcgc cgtacttcac
     2881 gccgaacagg cgcgtaccga agccgagtga aaatacctca acccgaaccc cgcacagttt
     2941 ggccacggcg aagtgcccaa actcgtgcac gagcaccata atcccaagaa caatcagaaa
     3001 cgaaatggtc gcagtaacgg cgaatggcat aatcaggagt ttgttcgatc ggcgaaaccc
     3061 gtaagaacct cggcagtaaa cccaggtgtc tctggactca gcaacgacga gccgctcctc
     3121 ggcccgccaa aacttcctgc gcctgctcgc gcgcctgccg gtccagcgcg agcacttctt
     3181 ctatggtcgc cggatgcgtc tccggggttg tctggagtac ctgttctatt gtatctggaa
     3241 tccctgtaaa gggaatgact ccttcgagga acgcttcaac cgccacctca tcggctgcgt
     3301 tcagcgcgat tgggtgcgcc ccgccctttt ccgccgcctc gtaggccagc ctaaggcacg
     3361 gaaaacgttc catatccggc ggttcgaagt gcagatcgct caaggccgcc acgtcgaacc
     3421 gcaactccga ctcgacccgc tccggatagc tcagcgcata cagaatcggc agccgcatgt
     3481 cggtcaccga aatctgcgcc agcaggctcc cgtccacaaa ctccaccagc gaatgcaccg
     3541 tcgactgcgg atgcaccgtc acctgcacct gcgacggcgg cagatcgaac agccggcacg
     3601 cctcgatgat ctccagtcct ttgttcagca atgtcgccga atcgatcgtg atccgcttcc
     3661 ccatcaccca cgtcggatga tgcagcgcct gctccaccgt gattcggctg aattcgctcg
     3721 ccggcagagt acgaaacggc ccgcccgacg ccgtcagcca gatctgccgc acctcgcgcc
     3781 gttcgcccgc gcgcatgcac tggtggatcg cgttgtgttc ggaatcgatg ggcagcagcg
     3841 ccactttctt cgcgcgcgcc gcagccgtca ggatctcccc cgccgccacc atcgcctctt
     3901 tattcgcgag gccaatcgtc ttgcccgcct gcacggccgc gtgcgtggcc tccagtcccg
     3961 ccacgcccac aatcgccgag acgacaaagt cgacttcctt cagcgtcgca acccgcaccg
     4021 tgccggcgct gccccacacc acttccgtat ctacacccgc cgcgcgcagc cgcgccgcca
     4081 gatcccccgc aagctcttca tccgcgagcg aaaccacccg cggacgccac cgtacgcact
     4141 gctcgaaagc cgcatccacg ttccgccccg cggccagcga ggccacggtg aaccgctccg
     4201 ggtagcgcgc aacaatatcc agcgtgctcg tcccaatcga gcccgtcgat ccaagaatgg
     4261 caatttgctt caaggaagaa attttacgct gaccgcttct tcgctgacct gttttcggga
     4321 gaccgcttca ccagctgcgt ttctcaaaac agctgctggg cgagctgggc gtaccacagc
     4381 acgggagcag ccagcaacag cgcgtcgatc cggtccagga acccgccatg ccccggcaac
     4441 agcttgccgg aatccttcgc acccgccgcc cgcttgatcg ccgactccgc cagatcgccc
     4501 acctggccgg caatgttcag cagtaccgcc agcacgatcc agtgcgcata agtccctgga
     4561 taggacaggt acatgatgga ccgcgccgcc agcgtccctc ccagccaaac cagcagcaca
     4621 gcgaccccga tgctcgccac cgccgatgcg atcgagccct cccatgtctt gttcgggctc
     4681 agccgcttcg ccagcgtcca tcgccccatc gagcgaccca catacagcgc agcgatgtcc
     4741 cccgcccaaa ccacaaacag caggaacacc aacagggacg gaccgttgtc ctgagccgac
     4801 agcagataca tcatcgacaa cggcagtccg atgtacaaaa gtccgaagac cgaaaacgcc
     4861 gtgtccggca gcacgcgctc cagcggcgag cgaaatgtgc agatcacgag catcaggagc
     4921 gccagcgctc cgctgatcgg agccatgagt tccggattgc gaaagatggc ggccatctgt
     4981 acggccagaa gaaccgtgac cacgattttg ggcgttttcg cgccgatcgc atcggccagg
     5041 cccagatatt cccacaatgc gagcgccgca atcacaaaaa gcgcccccat cagcacgatg
     5101 ggccatcgcg gcgaaacgta aatcaccgca atcacaagag gaatcaggac cacggccgtc
     5161 agaatgcgct tcatgccaca ggcagaatac aggaacagtt gtcagtcttc aggctccgca
     5221 gttgtcagtt gttagtcccc ggtttcaaga agaccgggga ctggagacgg aagactgagc
     5281 cgcagtactc agttgtcagc cgtcagccgt cagttgtcag ttgtcagtcc tcagttggta
     5341 gtgctcagtt gttagtggtc agtgatcacg gagcccatct ctcgtgtcgc gtcggcacaa
     5401 cggtagaagc cacgggcttc agtccgcggc acaaccccac ggcagaagaa gggccttcag
     5461 gcccggcaag ctcgctgcgc tctgcgacct gaaccctgta ccctggctat ttcgcttccg
     5521 gaatccgggt cgccggcatg ctcctgagga cttcgagttc cttctgcacc ttcgcccact
     5581 tcttcgggtg cttcttcgtc tcaatcggcg cgttgggatt cgagtagtag gccagaatgt
     5641 tttgccgcaa tcccagcggc ggcatcgcgt tctgctccgt cacttcatgc agcagcgtcg
     5701 cgtacgtttt gtccgtcagc cgatacgttc cgggccgcgt cggatacccc gtatccaggt
     5761 ccaggtccgg caccgcaaat ccgtcattcc ccttctgcgc cagctccccc agcaactgct
     5821 ggtagcgatc gatcgtcgca ttgaccgctt ccacatagtt ttcttccgac tcccggctcg
     5881 gcccgcgaat cgccagatcc gacagaatcc cgatcttcgg cgtgatcagg atcaggaacg
     5941 ccacgattcg cgtcttaaag ctgaccttgt gcttgcgata ctgctcccag ccgttgtcta
     6001 ccgacgcttt cttcacccgc tccgcgaacg tgtggaaacc cggcaggtcc tggtcgggcg
     6061 gaaaactctt ccggtgcagc agcacttctg catacgagat gcgcggcagg aattgccgca
     6121 ctgcccagtc atagctggcg atcgcatgcc gctcgtggcc gacaattttg ttcagccgta
     6181 gcccgtaggt cgcgtagaac gcgcgctcca gcagcttcac gggcacctgg aacccaacat
     6241 tgcgcagata gactgccgga gcaaatcgcg cgtgactcag ctgatcgatg tcgaacgccc
     6301 actccgttcg tatgtgcgca tgggggtcct gctcgtacgt cacaaccggc ccgtactcct
     6361 tctcaagatc cgggaactcg atcgccaccg acggattcac cgcctcgcga tgcccaatcg
     6421 agtcgcccac ataatgcgac agcgccccca gcgcaaatgc cagttcgttc acgttgtgcg
     6481 cctggtggat caggttcgtg atgaagtcgc cggtgcgaac gtagtgcgcc agattgctga
     6541 acggcggatg cccaaacgga tagtaccccg cgtcctgaat cacacacccg ccgtaggcaa
     6601 aggcatgcgc ggtctccagt tccttctcgg tcgtgttggg atacttcgcc agcagtaccg
     6661 gcttgatgcc gttcacccac gtgatgtcga tgatcgtctc atgggtgaga aaagaaaagg
     6721 cgaaagccag tggcgcgacc agcagcaacc cagcgacgcc aacccacgca attcttccct
     6781 tcatcctgcc gccgttcctt gattgggttt tggggccgat taccagcagt aggatgcaca
     6841 ccgcaaaaag gggatgttca gaggcgattt tgggccgcgt gccagatgcg aaggacctca
     6901 accgcttcgt ccttcacgcg atacaccgca acgtagggca gcctcccaaa gacaagctcc
     6961 cgcgttcctt cctctcggcc gagacggccc agccgtggga acttctggag ggattgcacg
     7021 tttgcattca actcgcgcac ggtcgactcg gcgtagcccg gatgatgctc cttcagataa
     7081 gcggcaatcc gttcaagatc gtccgtcgca ttcggcgtcc accgaatctg catttctcag
     7141 gagggaaaca tccggtcgat gcgcgcgttg atttccgctt gttcgatgaa ctcgcctcga
     7201 tcggcctgcg caatgccggc gcgaaccgct tcgcggaaac gttgatcctc atcaagaagg
     7261 ttcaacgcgg catccttaat caactgctca ggatcagtgc ccacgactgc ggcaatcgcc
     7321 gccagccggg cttcctcatc gggagtgagt cgcacttcca tggcgccatt ataggacgaa
     7381 cccgccccgc cccccga
//
LOCUS       DASZYN010000419         2354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_20613, whole genome shotgun sequence.
ACCESSION   DASZYN010000419 DASZYN010000000
VERSION     DASZYN010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20613"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            403..2025
                     /locus_tag="VHE33_10855"
     CDS             403..2025
                     /locus_tag="VHE33_10855"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HVT97996.1"
                     /translation="MDKISVAQQNGARAMARYKPYDLNQAKMIPLSYADQIVEGSFEH
                     ALNEIVEEHLDLTVFEKRYANDDTGRPAYDPRVLLKVVLYGYYKGIISSRMLAEACRR
                     NVVFMALSADTRPHFTTLAGFVSELDREIVGLFRDVLLYASELGLIGKEHFAVDGCKL
                     PSNASKRWSGTHQELEEKRQKLERVAERIVERHRERDGQEKEGASAARDAKKVEGYRR
                     KVEQIKAFLKEGKKKLGPSGNEQKSNVTDPESAKMTSSRGVLQGYNGLAVVDDRAQIV
                     VHAEAVGSGYEGHLLAPLLEATRETFGIIEPGEDVLAKVKVTADSGFHSQAVLAAVER
                     TGANAYIADRDYRRRDPVFAGAGRHKKRDQKERALERRKQREQLPQKPQLFGIEDFVY
                     DEAKGLCICPAGHKLYHSGKHLLFNGYRVAHFKAPITACRNCPLRAQCLRHPERTVQR
                     SICIIKGREGARTSDKKSGAVERMMRKFDTPRGREIYSRRMGTVEPVFGNLQNKGMRR
                     FTLRGKTKVDAQWQLFTMVHNIEKIAGRRRAS"
BASE COUNT          491 a          597 c          870 g          396 t
ORIGIN      
        1 ctcatgcagc agcagcggcc gcgggcgtgg attgggaaga gcctattgga tgcggattct
       61 caatgagcgc ttcggggcaa ggtctctggc tggttataac gaacgcggat tctcgcgggg
      121 gaaacgtccg tgtgcgggta tcggatgcaa cgggcgagat cattgagatc agaacgttgc
      181 cgagatgaga gttgcacagt gtgacgagcg gctgcccaac agggcgttgc agctgacggg
      241 cttctgtgtt ggggggacgt gtctcgctgc cttgccggct tcgcccaagg cagcgctcgt
      301 gcagaccgtg acgtctagcc gcgtctgcaa gggcccgcag ctgaacgccg agtcgttgag
      361 gttgtagaaa aagtcggaag gccctgggaa aaggcttcgt cgatggataa aatctccgtc
      421 gcacagcaga acggagcccg agcgatggcg cgatacaagc cgtacgacct caaccaggcg
      481 aagatgatcc cgctgtccta cgcggatcag attgtggaag gctcgttcga gcacgcgctc
      541 aacgagatcg tggaggagca cctggatctc acggtgttcg agaagcgcta cgcgaacgac
      601 gatacgggcc gaccggcgta tgacccgagg gtgctgctga aagtggtcct atacgggtac
      661 tacaagggga tcatttcgag ccggatgctg gcggaggcgt gccggcggaa cgtggtgttc
      721 atggcgttgt cggcggatac gcggccgcat ttcacgacgc tggcgggatt cgtgagcgag
      781 ctcgaccgtg agattgtggg cttgttccgc gatgtgctgc tgtatgcgtc ggagttgggg
      841 ttgatcggga aggagcactt cgcagtggac gggtgcaagt tgccgtcaaa tgcgtcgaag
      901 cggtggagcg ggacgcacca ggaactggaa gagaagcgac agaagctgga gcgcgtggcc
      961 gagcgtatcg tggaacggca ccgggagcgc gatgggcagg agaaggaggg cgcgagcgcg
     1021 gcgagagacg cgaagaaggt ggagggctat cgacggaagg tcgagcagat caaagccttc
     1081 ttgaaggagg gaaagaagaa gctgggtccg agcggcaacg aacagaagag caacgtgacc
     1141 gacccggaga gcgcgaagat gacgagctca cgtggagtgc tgcagggcta caatgggctg
     1201 gcggtggtgg acgatcgggc gcaaatcgtg gtgcacgcgg aagcggtggg ttctggatac
     1261 gaaggacacc tgctcgcgcc gctactggaa gcgacccgag agaccttcgg catcatcgag
     1321 cctggcgaag acgtgttggc gaaggtgaag gtgacggctg acagtggctt tcacagccag
     1381 gccgtgctgg cagctgtcga gcggaccggc gccaatgcgt acattgcgga ccgtgactat
     1441 cgtcggcgcg acccggtttt tgcaggcgct ggtcggcaca agaagcggga ccaaaaggag
     1501 cgcgctctcg agcggcgcaa gcagcgagag caacttccgc agaagccgca gctcttcggc
     1561 atcgaagact tcgtctatga cgaagccaag gggctctgta tctgccccgc gggtcacaag
     1621 ctgtatcaca gcggcaagca tctgctattc aatgggtacc gagtggcgca cttcaaggca
     1681 ccgatcaccg catgtcgaaa ctgcccgctg cgtgctcagt gtctgcgtca tcccgagcgc
     1741 acggtgcagc ggtccatttg catcatcaag gggcgagaag gcgcgaggac ttcagacaag
     1801 aagagcggcg cggtggaacg catgatgcgc aagtttgata cgccgcgagg gcgcgagatc
     1861 tacagtcgga ggatggggac ggtcgaaccg gtgtttggca atctccagaa caaggggatg
     1921 aggcgcttca cgctgcgtgg gaagacgaag gtcgacgcgc agtggcaact cttcacgatg
     1981 gtgcacaaca tcgaaaagat cgctgggcgg aggcgggcga gttgagggcg gcgcgaagag
     2041 cgcggcgtgg ggccgccgag gcgcgtcgta gacgccagcg gggcggcgct gagccgaaaa
     2101 tgatcgcctg aggtggagcg gggcgccgcg gcgatcgtgc cgcggggttt ttctacgggc
     2161 tcgttaggca gactaccaag atcgtcaatg gcgtcgcgta tcgacgcaca gagacaagcg
     2221 ggcgcggcag agtggtctgt cttggtgtcc gcgggcatcc cgtttgagtc tgattcgcag
     2281 gagggtgccc gggcccgtcc ggtcgccgga agtgggcggt cttacggtct tacgccgtgt
     2341 catgcgggaa gatc
//
LOCUS       DASZYN010000420         4274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_149078, whole genome shotgun sequence.
ACCESSION   DASZYN010000420 DASZYN010000000
VERSION     DASZYN010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149078"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VHE33_10860"
     CDS             <1..550
                     /locus_tag="VHE33_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HVT97997.1"
                     /translation="IELHPMLTEQMLRRSPALAVLNNIASAHHEKCDSSGYHKRVRAD
                     LGDFAACILAATEIYVGMTTERADRAAFSADEAAAELRRLASEGALDPRATQVVLTAA
                     GHNESKAPAPGRTQNPGGLSPREVDVLRLAARGLTTQEIAERLFISPKTADHHIQHIY
                     NKIAVSTRAAAALWAMQNAVVQ"
     gene            793..1011
                     /locus_tag="VHE33_10865"
     CDS             793..1011
                     /locus_tag="VHE33_10865"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVT97998.1"
                     /translation="MGEVLRAEEGSLYPALYRMEEAGWIRAEWIRKDSGRRARIYELT
                     AAGKKQLGVEESRWQSVSVAVNRVLREA"
     gene            1019..3670
                     /locus_tag="VHE33_10870"
     CDS             1019..3670
                     /locus_tag="VHE33_10870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HVT97999.1"
                     /translation="MGSRISNALRGERLNREIDEELQAHIEEAIASGRDPGEARRALG
                     STLRVCEASHSIRAAGWLESVVSDVKFGWRQLCRNKVASAAAVLSLALGMGSCVAAFR
                     LIDALLWRPMPISGSSRLYVLSRQMTGLYGKPIEDGHWATPDYKLMRDAVRDQADLIA
                     VSDADRTDITWTTDEDVEKAHVAYVSGNMFPLFRLEPALGRLLSPADDRGPGASPYAV
                     LSWDYWNRRFGRDPRVLSRLVHIGDQSFEIIGVGPRDFTGTEKGTVADIFLPLSMNSL
                     ATQERFDWHRIFLMLKPGVSPKTALEPLRQHLSAVNHVFGSACSTCFRGETRASIDRF
                     LNQTLVLAPAGAGISDLQKEYRRLLGMLGLLAALVLLIACVNVANMMTAHAAGRAHEM
                     ALRISIGAGRRRLVQLILCQSALLAVLASVAGAFFAAWSAPFVLSLVNRPDNPARLAL
                     PGDWRVLLFGFGLMLLVALLLGLLPALRASSVRPVAALKGGEDPHSPRRLLRASVAGQ
                     VTFCFLVLFLSSLFVTSFRRLQSRPLGFSTERLLLLETVAGKGQLPVVWNQTAEALRA
                     VPGVDSVAISSWPLLGRIKINSDISINGAAPGPTPAFFLNVSPGWLSTMKIPLVSGRD
                     FSPQDTSPGAAIVNETFVKTFFPGQDPIGRTFKRGTHQPANKIVGVTPDVPDHDLREP
                     NRAVFYVPLNSIDEKSFPTAVRFATFAIHTEGQNPLALAGSLRQLIDKRHNGLRVSNI
                     TTQLDLIRDQTVRERMIAIIAAFFAAVALLLAGIGLYAVLNYSVLQRRREIGIRMAIG
                     SPRVDIVRIVTFDVFLMIAMGGCAGVALGFGAARAVASLFYQVKASDGAMIALPACAL
                     LLTALIATLPAVLRALRTDPTEILRVE"
     gene            complement(3824..>4274)
                     /locus_tag="VHE33_10875"
     CDS             complement(3824..>4274)
                     /locus_tag="VHE33_10875"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HVT98000.1"
                     /translation="KRQPGIGMGRDPERTPMPWDGSLSSGFSTGRPWLPIGDANQALN
                     VQSSRNDPGSILSFYRHLLRMRRAEPVLVLGGLTDLTAYDGVIQFLRSDTGKRVAVMM
                     NLSEQARTVAVKLGSIIACTDLRREGESVADAVELAPSEALMIELLP"
BASE COUNT          745 a         1442 c         1202 g          885 t
ORIGIN      
        1 tatcgagctt caccccatgc tcaccgaaca gatgctgcgc cgatcccccg cgcttgcggt
       61 gctgaacaac attgcgtcgg ctcatcatga gaaatgtgac agctccggct accacaagcg
      121 ggtgcgcgcc gatcttggcg attttgcggc atgtatcctc gcggcgacag aaatttacgt
      181 gggcatgacg accgagcgtg ccgaccgcgc ggccttttcc gccgatgaag ctgccgcaga
      241 actgcggcgg ctcgccagcg aaggtgcgct cgacccgcgc gcaacacagg tggtgctcac
      301 ggccgccggt cacaacgaga gcaaagcgcc agcgcccggc cggacgcaaa atcctggcgg
      361 gctctcacct cgcgaagtgg atgtgctgcg cctcgcggcc cgcggtctta caacccagga
      421 aatcgctgaa cggctcttca tctcgcccaa gaccgccgac catcacattc agcacatcta
      481 taacaagatc gcagtctcga cgcgtgcagc tgccgctctc tgggcgatgc aaaacgctgt
      541 cgtgcagtaa gtggacggtg atccgaatca tcaacgcacc accggggaag gttttgcggg
      601 aagtccccgg tcgttgtcag gaacctccgc cacagcctgc gttgacaccc atacccagat
      661 agctatataa agacgtctta atatggccag gaccgatgcg ctgcaagggt cactcgattt
      721 gctggtgctc aagatcttgt cgcggcggcc gcggcttcac ggctacgcca tcatggctgc
      781 catcgccgat acatcggcga agtattgcgc gccgaggaag ggtcgcttta cccggccctc
      841 tatcgcatgg aggaagcagg ctggattcgc gccgagtgga tcaggaagga ttcgggccgc
      901 cgcgcgcgca tctacgagct aaccgccgca ggcaaaaagc aactcggtgt ggaagagtcg
      961 cgctggcagt ccgtgagcgt ggccgtcaat cgcgttctgc gggaggcatg agatgtcgat
     1021 ggggtcccgt atctcgaatg ctctgcgcgg cgagcgcctg aatcgcgaga ttgatgaaga
     1081 gctgcaggcc cacattgaag aagccattgc ctcagggcgc gatcccggcg aagcacgccg
     1141 tgcattgggc tccacgctgc gtgtctgcga ggcgagccac agcattcgcg ctgcgggatg
     1201 gctcgagtcg gttgtctcag atgtgaaatt cggctggcgg cagctgtgcc gcaacaaggt
     1261 cgcgtccgct gccgctgtgc tgtcgctcgc tctcggcatg ggctcttgtg tcgcagcctt
     1321 ccgtctgatc gacgctctgc tttggcgtcc gatgcccatc tccggttcca gcaggctcta
     1381 cgtcctctcc cgccaaatga ccggtttgta tggcaagccg atcgaggacg gccactgggc
     1441 cacgccggat tacaagctca tgcgcgatgc cgtcagggac caggccgacc tcatcgccgt
     1501 cagcgacgcc gatcgcaccg acattacctg gaccactgac gaggatgttg agaaggccca
     1561 cgtcgcttat gtgtccggca acatgttccc gctcttccgc cttgagcccg cgctcggccg
     1621 cctgctctct cccgccgatg accgcggtcc cggcgccagt ccgtatgccg tcctctcctg
     1681 ggactactgg aatcgccgct ttggtcgcga tccccgcgtc ctcagtcgcc ttgtccacat
     1741 cggcgaccag agcttcgaga tcatcggcgt cggccctcgc gatttcaccg gcaccgagaa
     1801 gggaaccgtc gccgacatct tcctgcccct cagcatgaac agcctcgcca cccaggaaag
     1861 attcgactgg caccgtatct tcctgatgtt gaaacctggc gtcagcccga aaaccgccct
     1921 cgaaccgctg cggcagcacc tctctgcggt caaccatgtt ttcggaagcg cgtgttccac
     1981 gtgcttccgg ggtgagaccc gggcgtccat cgaccgcttt ctcaatcaga cgcttgtcct
     2041 ggctccggcc ggtgcgggca tctccgatct tcagaaggaa tatcgccgat tgctggggat
     2101 gctcggtctg ctggccgctc tggtcctgct catcgcctgc gtcaacgtcg cgaacatgat
     2161 gaccgcccac gcggctggcc gcgcccatga gatggctctc cgcatttcaa tcggcgccgg
     2221 ccgtcgtcgc ctcgtccagc tcattctttg ccagagtgcc ctgctcgcgg tcctggcttc
     2281 tgtcgctggc gcattctttg cggcgtggtc ggcgccgttt gtgctcagcc tcgtcaatcg
     2341 gcccgacaac cctgcacgtc tcgcgcttcc tggcgactgg cgcgtgcttc tcttcggctt
     2401 cggactcatg ctcctcgtcg ctctgctgct cggtttactt cctgcgctcc gtgcctcttc
     2461 ggtccggccc gtcgccgcgc tcaaaggggg tgaagacccg cattcgccac gccgtctcct
     2521 gcgcgcatcc gttgcgggtc aggtcacttt ctgttttctg gtcctctttc tgtcgtcact
     2581 gttcgtcaca tcgttccggc gcctgcagag ccgccccctc ggtttctcca ccgaacgttt
     2641 gctcctgctt gaaaccgttg ccggcaaagg tcagctcccc gtcgtctgga atcaaacggc
     2701 cgaggctctg cgggccgtcc cgggcgtcga ctcggtcgcc atctcaagct ggccactcct
     2761 gggacgcatc aagatcaaca gcgacatctc catcaacgga gcagctcccg gtccgactcc
     2821 cgcctttttc ctgaacgttt ccccaggctg gctctcgacg atgaagatcc ccctggtttc
     2881 cggccgcgac tttagcccgc aggacacctc gccgggagcc gccatcgtga acgaaacctt
     2941 cgtcaaaact ttttttcctg gccaggatcc tatcggccgc acctttaaac gcggtaccca
     3001 tcaacccgcc aacaaaatcg tcggcgttac ccctgacgtc cctgaccacg atctgcgcga
     3061 gcccaaccgc gccgtctttt acgttcccct caactccatc gacgagaaat ccttccccac
     3121 cgccgtccgc ttcgccacct tcgccataca taccgaaggg cagaacccgc tcgccctcgc
     3181 cggctctctt cgccaactca tcgacaagcg ccacaacggc cttcgcgtct cgaacatcac
     3241 cacgcagctc gatctaatcc gcgatcagac cgttcgcgag cgcatgatcg ccatcatcgc
     3301 cgcgtttttt gccgcggtcg cgctgctgct tgccggtatt ggactttacg ctgttctcaa
     3361 ctacagcgtc ctccagcgac gccgcgagat cggtattcgc atggcgattg gctcgcctcg
     3421 tgtcgacatc gtgcgcatcg tgactttcga cgtcttcctt atgatcgcga tgggaggctg
     3481 cgccggcgtc gcgctcggtt ttggtgcagc gcgtgctgtc gcgtcgctct tctaccaggt
     3541 caaagcaagc gacggagcaa tgatcgcgct gccggcgtgc gcccttctgc tgacagcgct
     3601 cattgccaca cttcctgccg tgcttcgcgc gctgcgcaca gaccctactg agatcctgcg
     3661 cgtggaatga agacgcgcaa aattagaatg ccctgcaatc aattgactgc agggcattct
     3721 tcatcggctt gtatcccgta ataagccagg ccacccggtg aaaccttgta ggcttgctgt
     3781 cgattgtcga agagctggcc tcacccactg cgctgagcgg gtgtcaggga agcaattcga
     3841 tcatgagggc ttcagagggc gcgagttcga cggcatcggc gactgattct ccctctcgtc
     3901 gcagatccgt gcaggcaatg atcgaaccca gcttcacagc aacggtgcga gcctgctcgg
     3961 aaagattcat catgacagcc acgcgtttgc ctgtatcgga ccggaggaac tggattacgc
     4021 catcgtaggc ggtcagatct gtgagaccac ccaaaacgag cactggctct gcgcgccgca
     4081 ttcgcaacag atgccgatag aaggacagga tcgaccccgg atcgtttctc gaggattgca
     4141 cgttcagggc ctggttggca tcgccgatcg gtagccacgg tcgcccggtt gaaaatcccg
     4201 atgagagcga accatcccag ggcatcggcg ttcgctccgg atcgcggccc atcccaatgc
     4261 ccggttgcct tttc
//
LOCUS       DASZYN010000421         5125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_919399, whole genome shotgun sequence.
ACCESSION   DASZYN010000421 DASZYN010000000
VERSION     DASZYN010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5125
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919399"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1509
                     /locus_tag="VHE33_10880"
     CDS             <1..1509
                     /locus_tag="VHE33_10880"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HVT98001.1"
                     /translation="DGWLDIYLTNGSRFDAHWPAGKEPTTHLYRNNRDGTFTDVTAKS
                     GLGRTGWQTGVCVGDYDNDGWDDLFCTFWGHNVLFHNNGDGTFADVTKKAGLRQEHGR
                     WGTGCTFLDYDRDGQLDLFVCNFVKLDPDRPFPPAVTAMCQWKGIPTMCGPRGLPGDT
                     NLLYHNNGDGTFTDVTEKAGILKPGPRYSITAVSCDFDNDGWPDIYVAVDSEPSILFR
                     NNHDGTFTDVAVMSGCAYNADGHEQAGMGIGVADYDCDGWLDIFKTNFADDTSDLYHN
                     NGDGTFNDLSSSAGLGTNSNYVAWGCGFVDYDNDGWKDIVQVNGHVYPEVDRYNFGEA
                     FKNPRLVYRNLGNGKFEDVSAQMGPGITERFSSRGAAFGDFDNDGGMDILILNMNDRP
                     SLLRNVGTRQNWITLKLIGTHCNRTAIGARVRVATGKHVQMDEVHSGTSVMSQSDLRL
                     HFGLGKAETADWIEVKWPTTQKIERFTSVQANRFLTIREGQGIIATYVPPGK"
     gene            complement(1521..3161)
                     /locus_tag="VHE33_10885"
     CDS             complement(1521..3161)
                     /locus_tag="VHE33_10885"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT98002.1"
                     /translation="MHKRKSERQRCARAALQVILLLAVLSGAAVAQGSSSPGSISGHL
                     VDANHNAIPNASIRLSGGASRLATSDISGGFEFHNIVPGRYRITAILSTGQTISEWID
                     VGSGATTQVALEFHQETTGASGGESSAALSQSMQFADDPHFTIAGIADWTAAGGHGSD
                     ASLRTSEALTRETLKLAPDNNSPPVRPCTEGEAALKAAVASQPRSFEAEHCLGLFYLR
                     SRKYADAVRLLETAYSLERSDAENEYALAEACKRAGESAGARKHLENLSAHANAADLH
                     RLAGLLNESLGDPVTAVNEFAQAARMDPSEQNYFAWGSELLYHRAVLQARDVFEQGVR
                     AWPKSARMLTALGVAFFSAALYDDAAGRLCQAAGLEPDNPEPYLFMGRIVLVAPRPLP
                     CVAQKLAEFHRRHPENALADYYFAMALREQQPSADSPAEQQVAELLHEAVTADPRCGE
                     AWLELGNLQARSGNYASAISLYSKAIDAKPDLSEGYYRLGMAFDRTGQSAKAREAFAK
                     HDELRQLQAAEVEQQRREIKQFVISSAGTDTAGKNPRE"
     gene            3443..>5125
                     /locus_tag="VHE33_10890"
     CDS             3443..>5125
                     /locus_tag="VHE33_10890"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HVT98003.1"
                     /translation="MKPFSRRDLLKSSIVAPAAAVALHQMGPLGMAMEKGAETAPSTP
                     AQNAANTPLTASDRERLLLDFGWRFHFGNANDPSKDFGLGSGRGGDFQKTGNFLPASN
                     PAFDDADWTPVDLPHDWAIELPFTNDPALASKGFYPLGRAYPSTSVGWYRRVFELPAS
                     DAGKRISLEFDGVYRETLVALNGFYIGRHSGGYDPFSFDVTDFVNPGAINVLLIRVDA
                     TLSDGWFYEGAGIYRHVWMVKTSPVHVKKWGTFVASEVRSGEAALSIRTEVENQSKSA
                     HSIRVVSTVLDSSGRTVASATSSPQNVADGAEQTYEQKLTVQRPQLWSLEERNLYKLV
                     TELRADDATVDRYETPFGIRSITIDPQKGVFLNGQPVQIKGTCNHQDHAGIGAAVPDS
                     VQYYRVRKLQEMGCNSLRTSHNPPTSELLDACDQLGMLVFDETRMMSTNPEGLNQFAN
                     LIRRDRNRPSVFMWSMGNEERVANSERGVLILSGMKALAEQLDGTRPVSIAPTGAIGT
                     GGLAVCDVIGYNYMDPGALAYHQAHPDKPVIGTETVSAVGTRGIYITDPAKGY"
BASE COUNT         1098 a         1563 c         1461 g         1003 t
ORIGIN      
        1 gacggttggc tggacatcta cctgaccaac ggaagtcgct tcgatgccca ttggcctgcg
       61 gggaaagagc ccaccacgca cctctaccga aataaccgcg acggcacatt taccgacgtc
      121 acagcgaaat cgggattggg ccgcaccggc tggcagaccg gtgtgtgcgt cggggattac
      181 gacaacgacg ggtgggacga cctgttctgc acattttggg gtcataacgt tcttttccac
      241 aacaacggcg acggcacctt cgccgacgtc acgaagaagg ccggcctccg tcaggaacac
      301 gggcgatggg gaacgggttg cacctttctc gattacgacc gcgacggcca acttgacctg
      361 ttcgtatgta acttcgtcaa gctggacccg gatcggccat ttcctcccgc cgtcactgcg
      421 atgtgccagt ggaagggaat tccaactatg tgcgggccgc gcggtctccc cggcgatacc
      481 aacctgcttt accacaacaa tggcgacggg accttcaccg atgtaaccga aaaggccgga
      541 atcctgaagc ccggcccgcg atattcgatc acagcagtct cctgtgattt cgacaatgat
      601 ggctggcccg atatttacgt ggcagtggac tccgagccca gcatcctgtt caggaataat
      661 cacgacggaa cctttaccga tgtcgctgtc atgtcaggct gcgcatacaa cgcagatgga
      721 cacgaacagg ctgggatggg catcggagtt gcagactatg actgtgatgg atggctcgac
      781 atcttcaaga cgaattttgc cgacgacacc tctgacctgt accacaacaa tggcgatggg
      841 acattcaacg acttatcatc cagcgccggg ttggggacta atagtaacta tgtggcatgg
      901 ggctgcggct tcgtcgacta cgacaacgat ggatggaaag acatcgtcca ggtgaacggc
      961 catgtctatc ccgaggtgga caggtataac tttggcgaag cattcaaaaa tccccgcctc
     1021 gtctatcgca atcttggaaa cgggaaattc gaagatgtct cggcacaaat gggaccggga
     1081 atcacggagc gcttttccag ccgcggcgca gcgttcggcg atttcgataa tgacggcggg
     1141 atggacatcc tgatcctgaa catgaatgat cggccgtcgc tgctccgcaa tgtgggcacg
     1201 agacagaact ggatcaccct caagctcatt ggaacccact gcaatcggac agccatcggc
     1261 gcgcgggtgc gggtagcgac cggcaagcac gtacagatgg atgaggtgca cagtggaacc
     1321 agcgtcatgt cgcagagcga tttacgactg cattttggac tgggcaaggc agagaccgcg
     1381 gactggatcg aagtgaagtg gccaacgaca cagaagatcg agcgttttac gagtgttcag
     1441 gcgaaccggt ttctgacgat tcgcgagggc cagggaatta ttgcgaccta tgttccgccc
     1501 gggaaatagt ctgttgctga ttactcgcgt gggtttttgc cggccgtgtc tgtgccggca
     1561 gaagaaataa cgaattgttt gatttcccgc cgctgttgct cgacctcggc cgcctgcagc
     1621 tgccgcagct catcgtgttt cgcaaatgcc tcacgcgctt tcgcagattg gccggtccgg
     1681 tcgaacgcca tgccgagccg gtaatagccc tcactgaggt caggtttcgc atcgattgcc
     1741 ttgctgtaga gcgaaatcgc cgaggcatag ttccccgacc gagcctgcag attgcctaac
     1801 tcgagccagg cttccccgca ccgggggtcg gccgtaacag cttcatgtag taactcggca
     1861 acctgctgct cagcgggcga atcggcggaa ggctgctgtt ccctgagtgc cattgcaaag
     1921 taataatcgg caagcgcatt ttccggatgg cgccgatgaa actcggcgag cttttgtgcg
     1981 acgcagggaa ggggacgagg cgcgacaagc acaatacgcc ccataaagag atacggttca
     2041 ggattgtccg gctcgagtcc ggcagcctga cacaggcgcc ccgcagcatc atcgtaaagt
     2101 gcagccgaaa aaaaggcgac gcccagagcg gtgagcatgc gtgcagattt cggccaggct
     2161 cggactccct gctcaaaaac atcgcgcgcc tgcaagacgg cacgatggta gagaagttca
     2221 gagccccacg caaaataatt ctgttcgctc ggatccattc gagcggcctg tgcgaactcg
     2281 tttacggccg tcacggggtc gccaagcgat tcgttcagca ggccggccag acggtgaagg
     2341 tcggccgcgt ttgcatgagc agagagattt tccagatgct tccgcgctcc ggctgattcg
     2401 ccggcccgct tacaggcttc agcgagggca tactcattct ccgcatccga gcgctcgaga
     2461 ctgtacgcgg tctccaggag cctgacggcg tcagcatact tccggctccg caaataaaac
     2521 aggccaagac aatgttctgc ctcaaaactt cggggctggc tggccaccgc ggctttcaga
     2581 gcagcttcgc cttcggtaca tggacgaacc ggcggagagt tgttatccgg agcaagcttc
     2641 agagtttctc gagtcagagc ttcgctggtg cgaagactgg catcggatcc gtgaccacct
     2701 gctgcggtcc agtcagcaat ccccgcaatg gtgaaatgag gatcatcggc aaactgcatg
     2761 gattgagaga gcgcagccga agattccccg cccgatgctc cggtggtttc ctggtggaat
     2821 tccagggcta cctgggtcgt tgcccctgaa ccgacatcga tccactctga aatagtctgg
     2881 ccggtggata ggatggccgt gatgcggtag cgtcccggaa cgatattgtg aaattcaaaa
     2941 ccaccactta tgtcagacgt tgcgagtcga gaggcgcccc cgctcagtcg aatcgatgcg
     3001 ttggggatgg cgttgtgatt ggcgtcgacg agatggcctg aaatggagcc aggtgaagaa
     3061 ctcccctggg caacggccgc gccggagagg acagcaagga gcagaatgac ctgcagggcg
     3121 gcgcgggcac agcgctggcg ttctgatttc cgtttgtgca tccgaaatcc tcacggccgc
     3181 gtacaggatg atcgacttgc cggcccccgg actcgcagcg gctgcagcgc gtgatgccca
     3241 gtatcgcgga ttccgaattt ggagcgcaac aacctcccat catcgtgcac ctgattcagc
     3301 catttgcgat gccggttcgc attggcgtat tttcttcgtg acgtatactt ggcgggctcg
     3361 gaaaacctgg taaaaccgtg gcgtccttaa agccagggga tcgccgggat tccgttcgtt
     3421 gaaaggttga gctggaggct cgatgaaacc gttttcacgc cgtgatcttc tcaagtccag
     3481 tattgttgca ccggctgcgg ctgttgccct tcaccagatg ggacctctgg gaatggccat
     3541 ggagaaaggc gcagagaccg caccttcaac tcctgctcag aacgcggcga atacgcccct
     3601 caccgcctcc gaccgcgagc gcctgctcct cgactttggc tggcgctttc acttcggcaa
     3661 cgccaatgat ccctcgaaag atttcggcct cggcagcggc agaggaggag acttccagaa
     3721 gaccggcaat ttccttcctg catcaaaccc cgccttcgac gacgccgact ggacgcctgt
     3781 ggacctgccc cacgactggg ccattgagct gcccttcacc aatgatccgg ctctcgccag
     3841 caagggtttt tatcccctcg gccgcgccta tccgtccacc agcgtcggct ggtatcgccg
     3901 cgtcttcgaa cttccggctt ccgacgccgg caaacgcatc agccttgaat tcgacggcgt
     3961 ctaccgcgaa actctggtcg ccctgaacgg cttttacatc ggccggcata gcggcggcta
     4021 cgaccccttc agcttcgacg taacagactt cgtcaaccca ggcgcaatca acgtcctcct
     4081 catccgcgtc gacgccacac tgagcgacgg ctggttctac gagggggccg gcatctatcg
     4141 ccatgtctgg atggtgaaga ccagtcccgt ccacgtaaag aaatggggaa cgttcgtcgc
     4201 ctccgaagtg cgctccggcg aagccgcact ctccatccga accgaagtcg aaaaccagag
     4261 caaatctgcg cacagcatcc gcgtcgtttc caccgtactg gattcctcgg gccgcacagt
     4321 cgcctcggca accagttcgc cccaaaacgt cgccgacggt gcagagcaaa cctacgagca
     4381 aaagctgacc gtgcagcgcc cgcagctctg gtcgctcgag gagcggaatc tctacaagct
     4441 cgttaccgaa cttcgcgcag acgacgccac cgtcgatcgc tacgagactc cgttcgggat
     4501 ccgcagcatc accatcgatc cgcaaaaggg tgttttcctc aacggccagc ccgtccagat
     4561 caagggcacc tgcaatcatc aggatcacgc cggcattgga gctgccgttc ctgactccgt
     4621 ccagtactac cgcgtgcgca agctgcagga gatgggctgt aactccctgc gcacctccca
     4681 caatccaccc acatcggaac ttctcgatgc ctgcgaccag ctcggcatgc tcgtcttcga
     4741 cgagacccgc atgatgtcca ccaaccctga aggcctcaat caattcgcga acctcatccg
     4801 tcgcgaccgc aatcggccca gcgtcttcat gtggtccatg ggcaacgaag aacgcgtcgc
     4861 caactcagag cgcggcgtcc tgatcctttc cggtatgaaa gcgttggcgg agcaactcga
     4921 cggcactcgt cccgtctcca tcgcccccac gggcgcgatt ggcaccggag gcctcgccgt
     4981 ctgcgatgtc atcggctaca actacatgga tcccggcgcg ctcgcctatc accaggccca
     5041 tccggataag cccgtcatcg gcaccgaaac cgtcagtgcc gtcgggacgc gcgggatcta
     5101 cattaccgat cccgccaaag gctac
//
LOCUS       DASZYN010000422         5559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_273665, whole genome shotgun sequence.
ACCESSION   DASZYN010000422 DASZYN010000000
VERSION     DASZYN010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273665"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VHE33_10895"
     CDS             <1..253
                     /locus_tag="VHE33_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98004.1"
                     /translation="EKNWPGLRGYMLRPFFHDAKNQFAIVWLFATVEDRNRNFDAQDR
                     ANDLERAALAKVKPFEDRFKARYGDYTVTYTHDDDWVVQ"
     gene            343..720
                     /locus_tag="VHE33_10900"
     CDS             343..720
                     /locus_tag="VHE33_10900"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVT98005.1"
                     /translation="MARTAHPDDVFSAIAEPRRREVIAVLANGREYAVNDIVLSVKMA
                     QPSVSKHLGALHRAGVVTMVRRGRHRMYRLNAAGLKPVHDWVKVFERYWTHQIDEIKQ
                     RAERKALERMIRLNDSSTEKKEE"
     gene            720..1172
                     /locus_tag="VHE33_10905"
     CDS             720..1172
                     /locus_tag="VHE33_10905"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HVT98006.1"
                     /translation="MAATVVEDVLQAFEIVKEEEIAAPIGIVFETILEQMGPLNATPE
                     KPMPMVLEPWPGGRWFRDLGNNDGHYWGAVQAIKAPSLLEISGPLFMSSPAVSNVQYR
                     LTEENGVTRMRFVHRAMGWIGEQDRRVDEGWGDLMTRIRTAATARRAR"
     gene            1305..2057
                     /locus_tag="VHE33_10910"
     CDS             1305..2057
                     /locus_tag="VHE33_10910"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HVT98007.1"
                     /translation="MIPNEQIAAMKKPPVVSAEEWQKAWAEMLVKEKELTRSRDALAA
                     ARRRMPWLAVEKNYVFEGPLGKASLLDLFEGRRQLVLYRAFYDPNVYGWPDHACMGCS
                     MGADQVSHLSHLHARNTTLAYASRGSQENLALLKQRMGWEKIPWYTITDDFDKDFGVD
                     QWHGHNVFFRDENNRIFRTYFINARGDESMGSVWSYLDATPLGRQEDWEDSPQGYPKT
                     SRYKWWRWHDSYGKEDAAWDQVVDNASALLNL"
     gene            complement(2249..2869)
                     /locus_tag="VHE33_10915"
     CDS             complement(2249..2869)
                     /locus_tag="VHE33_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98008.1"
                     /translation="MNARVRKLMLMMGAVAVGALWVPMASAVCANPFLKAHVAKQAWD
                     GQVGGAAQLLMASDHDHDAGIVGLWHVAFIAEGNTGAGLPPDGAQVDSGLTMWHSDGT
                     EETLDSRPPATGDVCYGVWKQVGERHYLLNHFGIAFDPSTDPNTPLGYAHIPQDVVVS
                     RDGRTFTGTFVIDQYDATGNLLIEIKGKLVGTRITLGTTVGDLVGS"
     gene            3151..5325
                     /locus_tag="VHE33_10920"
     CDS             3151..5325
                     /locus_tag="VHE33_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HVT98009.1"
                     /translation="MVRFGPYEVRPSTRELFRHGLRIKLPPQAFEVLRVLLERPGELV
                     TRQEFHRILWAADTFVDFDQGLNNAIKRIREVLNDSADAPRYIETLPRLGYRFIGTVE
                     RAEAEMASPAGQNGHAPAPASALSAKPALAPELEVEAPAPASRRKSLPGLMLVAAAAL
                     ALLPVTWLFRPSYPEPRITGEAQLTADGIPKWGPLVTDGLRVYFTERLNGKETIAAVP
                     ISGGQAVPLRTPFDQAGLYAISPNRNDLLIAETHDMHRDAPLWRLPIIGGTPRRLGTL
                     AAHDASWSPDGTRLACTIGSAVYIANADGSDPRILVPSTGVQNELAWHPTWSPDSRRL
                     RFDYFEMQRTEAKIWEVNVDGTNLHPLFASPQDWPMQAYGTWTADGRFYIFSAWKEKE
                     SSTPTAAANLWAVREKVGLVHRTSPSPNSLTAGPIRYFFHTPSLDGETIFALSSLKHG
                     ELMRYDPHSKRLSLYASDFSAEGVSFSPDGAWMVWVKFPQGELWRSRTDGSEPLQLSS
                     RPLFVTSPVWSPDGRQIAFAGMRAGETWRSYTVSADGGDPRPIGAIGEAQDPAWSPDG
                     NSLVYVDTSHGDDNTLIQIRNLRSGDVTSIPGSRGFWSPRMSPDGRWIAALSNNIRKL
                     LLFDSHTRTWKEVATADGIIVWPQWSRDSTYLYFSYGDPEPHIFRINVNGGKPEAILT
                     LKDFRTTGVLPGWFSVAPNGDILLLHDAGGGTEIYALSWDAP"
BASE COUNT         1099 a         1861 c         1524 g         1075 t
ORIGIN      
        1 cgaaaagaac tggcccggcc tcaggggata catgctgcgg ccgttcttcc acgacgcaaa
       61 gaaccagttc gccatcgtct ggctcttcgc caccgtcgag gatcgcaacc gcaatttcga
      121 cgcccaggat cgcgccaatg atctcgaacg cgccgctctg gccaaagtga agcccttcga
      181 agatcgcttc aaggcccgct acggcgatta caccgtcacc tacacccacg acgacgactg
      241 ggtcgtgcag taagccatac ccgcgtcgct cccctcggga aactccagcc cattcgtcac
      301 atcctctctt gacatattcc tatataggaa tacattggcc ccatggccag aaccgcgcac
      361 cccgacgacg tcttctctgc catcgccgaa cccagacgcc gcgaggtcat cgccgtcctg
      421 gccaacggcc gggagtacgc cgtcaatgac atcgtcctca gcgtgaagat ggcccagccc
      481 tccgtctcca agcacctcgg agccctgcat cgggcgggcg ttgtcaccat ggtccgccgg
      541 ggacgccatc gcatgtaccg gctcaacgcc gctggcctca agccggttca cgactgggtg
      601 aaggtctttg agcgctactg gacccaccag atcgacgaaa tcaaacagcg cgccgagcgc
      661 aaagccctcg agcgcatgat tcgcctcaac gacagctcaa ccgaaaagaa ggaggagtaa
      721 tggcagcgac cgtagtggaa gacgttctgc aggccttcga gatcgtgaag gaagaggaga
      781 tcgcggcgcc cattggcatc gtcttcgaaa ccatcctcga acaaatgggc ccgctcaacg
      841 ctactcccga aaaaccgatg cccatggtgc tcgagccgtg gcctggcgga cgatggtttc
      901 gcgacctcgg caacaatgac ggccattact ggggagccgt tcaggctatc aaagcgccca
      961 gcctccttga gatctccggt cccctcttta tgtccagtcc cgccgtctcc aacgtgcagt
     1021 accggctgac cgaagagaac ggcgtgaccc gcatgcgctt cgtgcatcgc gccatgggtt
     1081 ggatcggcga acaggaccgc cgcgtcgacg aaggatgggg tgacttgatg acacgcattc
     1141 gcaccgcggc cacagccagg agagccaggt agccatgtgc cccttctgca ttgcgaccgc
     1201 ggtctggatt gcggccggtg ctgtctcgac cggcggcctc tcggccctcg ccctttcaaa
     1261 gctcaataag aaaaaacccg acgaacctca acaaggagtt gctcatgatc cccaatgaac
     1321 agatcgcagc catgaagaag cctcccgtcg tctcggcgga ggaatggcag aaagcctggg
     1381 cggagatgtt ggtgaaagaa aaagaactca cccgctcccg tgacgctctg gccgcagcgc
     1441 gccgccgcat gccctggctc gctgtcgaga agaactacgt cttcgaaggt ccgctgggca
     1501 aagcctccct cctcgatctc ttcgaaggcc gccgccagct tgtcctctat cgcgccttct
     1561 acgatcccaa cgtctacggc tggcccgatc acgcctgcat gggctgctcg atgggcgccg
     1621 accaggtctc gcacctctcc cacttgcacg cgcgcaacac cacgctggcc tatgcctccc
     1681 gcggatccca ggaaaacctc gccctcctca aacagcgcat gggctgggag aagattccct
     1741 ggtacaccat caccgatgat ttcgataagg acttcggcgt cgatcagtgg cacggtcaca
     1801 acgtcttttt ccgcgacgag aacaacagga tcttccgcac ctacttcatc aacgctcgcg
     1861 gcgatgaatc catgggcagc gtctggagct atctcgacgc caccccgctc ggccgccagg
     1921 aggactggga agattccccg cagggctatc cgaaaacatc gcgttataaa tggtggcgct
     1981 ggcacgactc ctacggcaaa gaagatgccg cctgggacca ggtcgtcgac aacgcctccg
     2041 ccctcctcaa cctgtgacgc tcgcagggcg atccgcttcc ggtctccaga ttcgcttcaa
     2101 cccggcatcg gcagccctgc cacaaccacc aaagaaaaag gctccgggga caccgctccg
     2161 gaaaaattcc aggacggtgt tcggaccccg gaagcctcat gaggaaatcg gttcgggccg
     2221 aaatggctgt cgtctccgtt ctgcttcgtc agctgcccac cagatcaccc accgtcgtgc
     2281 ccagcgtgat gcgtgtgccc accagcttgc ccttgatttc gatcagcaaa tttcccgtcg
     2341 catcatactg gtcgatgacg aacgtgccgg tgaacgttct gccgtcgcgg ctgacaacca
     2401 cgtcctgagg gatatgcgcg taacccagcg gcgtattggg atcggtggac ggatcgaagg
     2461 cgatcccgaa gtgattcaac aaatagtggc gctcacccac ctgtttccat accccgtagc
     2521 agacgtctcc cgttgccggc ggacgcgagt ccagtgtttc ttccgtgccg tcgctgtgcc
     2581 acatggtaag tccgctgtcc acctgggctc cgtccggggg aagccccgcg ccggtgttgc
     2641 cctcggcgat aaaagccacg tgccacaggc ctacgatgcc ggcgtcatgg tcatggtccg
     2701 aagccatgag gagctgcgca gcccctccca cttgcccatc ccacgcctgc ttcgccacgt
     2761 gcgccttaag gaaggggttg gcacaaacgg cggacgccat cggaacccac aaggctccca
     2821 cggcaaccgc ccccatcatc agcatcagtt tcctgactcg tgcgttcatc gagtttcctc
     2881 caccagggat tgaagagtct ctgcccggac ctgaaccccg atgttgtatc caacggcgct
     2941 gaggccagac cgggcgaatc tcctcgatac ctgggctggc gcgcaatctc tgatgtatta
     3001 gttccggctg ttttttgcct aagtatgcaa gttactgacc tacaaggtgc ttgcacgcta
     3061 gcgaggctat cctgcctgcg aaatcaggct cgcatgctat acttccgccc caacgtcctt
     3121 tagtgtcggc caacgtggag gcctctttgt atggttcgct tcggccccta tgaggttcgc
     3181 ccttccacgc gcgaactctt tcggcacggc ctccggatca agttgccgcc tcaggctttt
     3241 gaagttctgc gcgtcctgct tgagcgtccc ggcgagctcg tcacccgcca ggaattccac
     3301 cggatccttt gggccgccga caccttcgtc gacttcgacc agggcctgaa caacgccatc
     3361 aagcgcattc gcgaagtttt gaacgattcg gccgacgctc cccgctatat cgagaccctg
     3421 ccccggctgg gttatcggtt cattggcact gtcgaacggg ccgaggcgga gatggcaagt
     3481 ccagcgggtc aaaacggcca cgcgcccgct cccgcatcgg cgctgtcagc aaagccggcg
     3541 ttagcccctg aactggaagt cgaagcgccc gcgcctgcca gtcgtcgaaa gtcacttcct
     3601 gggctgatgc tggtagcggc agcagcattg gcgctgttgc ctgtgacctg gctgtttcgt
     3661 ccttcctatc ccgagccccg tattactggc gaggcccagc tgaccgccga cggcataccg
     3721 aaatggggcc cgttggtcac cgacgggctg cgcgtctatt tcacggagag gctgaacggg
     3781 aaagaaacga tcgccgccgt acccatctcc ggcggacagg ccgtcccctt gcgaacgcct
     3841 tttgaccagg ctggtctgta tgcgatttct cctaacagaa acgacctgtt aatcgcagag
     3901 actcacgata tgcaccggga tgctccgttg tggcgcctgc cgatcatcgg aggcacgccg
     3961 cgccgcctgg gcacgctcgc cgcccatgat gccagctggt cgcccgacgg tacgcgactc
     4021 gcctgcacga ttggctccgc tgtctacatc gccaacgccg atggcagcga tccccgaatt
     4081 ctggtgccat ccactggtgt tcagaacgaa ctcgcgtggc accccacctg gtcccccgat
     4141 agccgtcgcc ttcgcttcga ctacttcgaa atgcaaagga ctgaggcgaa gatctgggag
     4201 gtcaacgtcg acggcaccaa cctgcatccg ttatttgctt ccccgcagga ttggccgatg
     4261 caggcctatg gaacctggac cgcggacggc cgcttctaca tcttctccgc atggaaggaa
     4321 aaggaatcgt ccaccccgac ggctgcagcg aacctgtggg ccgtacgcga aaaggttggg
     4381 ctcgttcacc ggacttcgcc ttcaccgaat agtctgactg ccggtcccat tcgctatttt
     4441 tttcatactc cgagcctcga cggcgaaacg atctttgctc tcagttctct gaaacatggc
     4501 gaattgatgc gatacgatcc gcattcaaag cgcctctcgc tctacgcgtc tgatttctcc
     4561 gccgaaggag tctctttctc cccggacggc gcctggatgg tctgggtgaa atttccccag
     4621 ggagaactgt ggcgaagccg cactgacggc agcgagcccc tgcagctgtc ctcgcgcccc
     4681 ttattcgtca cctccccggt atggtctccg gatggccggc aaatcgcctt tgccggaatg
     4741 cgcgctggcg aaacatggcg cagctatacc gtctccgccg atggcggcga tccccggcca
     4801 atcggagcaa tcggggaagc acaggacccg gcctggtctc cggatggaaa ctcgctcgtt
     4861 tacgtcgaca cctctcatgg tgacgacaac accctgatcc agatcaggaa tctgcgttcc
     4921 ggagatgtca cttcaattcc aggctcgcgc ggtttctggt ctccacgcat gtcgcccgat
     4981 ggccgctgga tcgccgcact ctccaataac atccgcaagc tgttgctgtt tgactcacac
     5041 acccgaacat ggaaagaagt ggcgactgcc gacggcatca tcgtctggcc gcaatggtcg
     5101 cgcgacagta catatctcta cttttcttat ggcgaccctg agcctcacat cttccgcatc
     5161 aatgtcaacg gcggcaagcc agaggcgatt ctgactttga aggacttccg caccaccggc
     5221 gtcctgcccg gttggttttc cgtcgccccc aacggcgaca tattgttgtt gcacgatgcc
     5281 ggtggcggca ccgagatcta tgcgctttcc tgggatgctc cctagcgctg aacgggacgc
     5341 tgccctgcct cccttcttga caaacccgca acgcacctga cagcatgggt gccgcagccg
     5401 aggaggtcag accactcacg gcccaactcc aaaggagcac cgacccaaca tggcggaatc
     5461 aacgcgcagg gaatttgtca cagcaggagg cgccgccctc gcggctgctg ccgtttcgtg
     5521 gaatgccaag agctacgccg ccatcgccgg cgccaatga
//
LOCUS       DASZYN010000423        12195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1175376, whole genome shotgun sequence.
ACCESSION   DASZYN010000423 DASZYN010000000
VERSION     DASZYN010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 12195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12195
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175376"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..116)
                     /locus_tag="VHE33_10925"
     tRNA            complement(31..116)
                     /locus_tag="VHE33_10925"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(80..82),aa:Leu,seq:gag)
     gene            495..1457
                     /locus_tag="VHE33_10930"
     CDS             495..1457
                     /locus_tag="VHE33_10930"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT98010.1"
                     /translation="MQLPLRRLLLIIGLCVASTWTAEGQSTPGVQDGVALMQQGRLEE
                     ARAIFQSVLQRDPQNQQAQDQAVAATERLALEQRAGNHTVDALQELMDGQKLVPSSAR
                     LAYDIGILEDEMQLYPEAARALETAKQLHMDDPMLLYAMARVQMDAGQLETAAQTMQQ
                     YLQVRPGDASAHFGLGRIYQIGMKFDKAVPEFQESIRLQPVQSEAWYQLGDIALKQHE
                     FDEALTDFNHTLERNPRHGGALAGSGECLFREKRYEKALKFLDRAIAAAPDYQTGHYY
                     RGLTLARLGRKEESEKELATATALADAENKKSATRYQLTVTGQP"
     gene            complement(1571..2506)
                     /locus_tag="VHE33_10935"
     CDS             complement(1571..2506)
                     /locus_tag="VHE33_10935"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT98011.1"
                     /translation="MRLRFWISPILAAVLSGSCAIAQQLNPDKAERRPALPLTAEEPA
                     ISPSLQVTEARLRDALRNDPKSVEALYDLALVLRQEVKPKESLALYTQAAALRRPTAN
                     ELRSVALDYVLLNDYDDAVHWLRIAFSTDPSNADVLYSLGRCLYTQNHFDQAELAFRR
                     VLTLRPEHLKAEENLGLTLDAENQTDKAEAALRQAAQWATERKTTDPWPFLDLGVFLL
                     EQQRAAEAVPSLKTAAALAPDLAEAREKLGRALSLTGDAKAGASELEAAVRLDPKDPK
                     AHFELGQAYRNLGEADKARAEFALCETLYGKHSRD"
     gene            complement(2676..3803)
                     /locus_tag="VHE33_10940"
     CDS             complement(2676..3803)
                     /locus_tag="VHE33_10940"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT98012.1"
                     /translation="MAKTLLCFVALWSAAMLQCLPAMSAGTQTPPGSEAELLRQGAQA
                     IHEGRFADAELRFGDAVRAYPDSPNAYLGLGMTLLREGHGDKAEEPLEHAIRLRADLP
                     GAHLFLSIAQYQQNQFDAAVDSLKTELQIQPNNTEVLSWLGIVEMARGDASAAVGPLD
                     RAGALAPKDANILDLRARAHTQVARECFQQLYALDPKSWHVHRALAEIYSAGGQPERA
                     AEEYAIAVKDQPNNADLYDALGVEYQRSGKLDEAAQAYGRALELNPHSTTALLQLGRI
                     DVEGADPESGIPLLREAIDAHADPVQAYYLLGVGLGKTGHDQEAVGALETCLKQHPSP
                     FIERAAWYALLPIYRRLNREADVRRAKAELDRLQAAGVAPR"
     gene            4391..8281
                     /locus_tag="VHE33_10945"
     CDS             4391..8281
                     /locus_tag="VHE33_10945"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT98013.1"
                     /translation="MTRKPRIAGFFRIMGVLLTLLSVPLVGFAQSARGSLAGTVTDPS
                     GAVIVGASVAATQNETGSKAETLTTSSGDFRFPELPLGRYDIVVTAAGFSKSTSTGVL
                     ITVGSISTLNVVLKTGSVAETVTVDASAPTLETASSDIGGTVSTKQIEDLPLSLALGV
                     NGMRSPETFEFLVPGTTGPGSATQSNSSNGVFFARISGGQAYGNETLLDGASVQRSEN
                     GSSFDETSPSIEALQEFKVTTSTPSAEFGRTTSGITSFSTKSGTNDFHGIGYAIVKNR
                     AFDANSWFNDGYKAYQCVGVSDLNCAYSKPADSKYDFGGIFSGPVKIPHLYDGRNRSF
                     FLFAWEQYKFKLGGTQYATVPTAAERTGDFSADLGPDIPGGSPYPGAPYNVLLNPCTG
                     TPLQYNQIFDPRTSTQISPNVFCRTPYPGNVINSPMSAAAQKLLSALPLPNQAPLSTD
                     LYGYRNNYVTSGTRSIVNTTYTIRIDQNIGLKSKIFATYDTRQNTALEGIPNFPEPYN
                     SNGYPQAFTTHYSRAGWDYSISPTVLNHLNLGYNRTNSVNVGATYGTSQTASSLGIAN
                     DFSKFFPLIVFPSPDQPTTWGQQQNGDNIDNGIRVNDSVSWIKGRQSLKFGVDFRNQQ
                     YSVIQYNQDTFNFYRDQTAGLSNACCGSGSSIASFLTGEVGNGFQAVYNDHPRWNSHY
                     IAGFFEDDLKLSKNLTVNLGLRYDVDVPRKEALNRTSAFSFTAPDAAAGNLPGALVFG
                     TNCSHCNTAWADTWWKDWGPRVGFAYVLPHTDDKVVLRGGGAILYGPLQYSDFGSSMS
                     LGYNQNRSFYSVQNAAANTGGAFTPAFRLDSSSTADPTNPSVGFPAVSYAPNLDPTQL
                     TAQNGPGSFAAVGGEVIRSNWGRPAMVDNWSLQLQDQVAQDLILTIGYIGQSGQNLHS
                     GYLSNINNIDPKYFSLGDKLNIPGYTIPLGGSNGGVDAPYASFTGALGQALRPFPQYD
                     YIAGDCCLENMGHSSYEALVTSLQRRFHDGLNLQASYTWSKALTDADSTIPFSYITGG
                     GGNTLRQGQGSANLHLEKAVSAQDITHQFSLSYLYQLPFGKGRKWLHNSRAADLLIGG
                     WQVGAIQRYQSGEPVSFGCASGIPFYQNCITYTAGPASKGGTSFASAAYKANKNGPSY
                     FNQKSWFKPAYNTIASSGPLVALSDAAFVDQNSEFGPDRTFTPGCAAFNGGNACSFDP
                     FSLGNIPRVTNKITGPNYLSEDVSLLKDFHITERLVFQLKGEGFDIFNRHRMAIPDTS
                     PGDLCNGTGCTGFGIPTATDYGPRNLQVSGRVTF"
     gene            8434..10350
                     /locus_tag="VHE33_10950"
     CDS             8434..10350
                     /locus_tag="VHE33_10950"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HVT98014.1"
                     /translation="MMSRVLLMSFICVVGISFVSPRFERAQQSQSNSSGMAADSAQPP
                     GFDAQHRPITAGGTVASGPIVFEDIADQAGLTRWRNITGTPQRRLIIEAKGSGVCLLD
                     FDRDGWLDIYLVTGSTFDALSGKGSAPHAALFRNNHDGTFTDVTAKAGVANDRWGMGC
                     AVADYDNDGWPDLYVTNLGGNRLFHNNRNGTFTDVAEQAGVALTSPPGSILTDYTGAT
                     FGDYDGDGRLDLFVAGYIQYDLRNPPVGGSSSVRTARCQYRGVDVMCGPRGLQGAPDH
                     LFHNNGDGTFTDVSRKAGVEDDAGYYGLGALIVDVNGDGLPDLVVANDSTPNYLYINK
                     GDGMFENQSYASGLAVNGEGREVANMGLAAGDYENNGQLDLVSTTFSDDYDVVFRNNG
                     RGEFEDVSYRTRIAQPTLPFVGFGDGFLDYDNDGWKDLFIANGHVYPTADHHPEWGTS
                     YAQRPLLFHNQHDGTFALVPAVQGTGLATLSVARGAAFGDLFNDGRVDVVINNLEGRP
                     VLLRNVNSDRNHWIELRLIGGSRSPRDAVGATVYVTAGGVRQRADVLSGGSYLSSSDL
                     RLHFGIGTSTHIDGIEIHWPDRGIEKIAVKSVDRIFTLVEGKGVTEQMPTQLAPQPRT
                     PAPNSAPKRPGMAS"
     gene            complement(10391..11062)
                     /locus_tag="VHE33_10955"
     CDS             complement(10391..11062)
                     /locus_tag="VHE33_10955"
                     /inference="COORDINATES: protein motif:HMM:NF008888.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HVT98015.1"
                     /translation="MELLEQVRVEAWSDEQVVLHVLAGDTALYEILMRRYNQRLYRTV
                     RAILREDDETEDVMQDAWVRAYQHLDQFEGRAAFSTWLTRIAVHEALARLRRRNRFSA
                     IDENDGEMDPPSAERDPEQNTAHSEVSGMLEEALLNLPEMYRTVVMLRDVEELSTAET
                     AASLEISEQNVKVRLHRGRALARKALMEKVGASAKTAFPFMGERCDRIVAGVFARLAD
                     QQRPR"
     gene            11177..11614
                     /locus_tag="VHE33_10960"
     CDS             11177..11614
                     /locus_tag="VHE33_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98016.1"
                     /translation="MERVDSRLNSAWWALRLGLGLGPIVAGLDKYFNKLTDWTMYLSP
                     LVTKVVPVSPATFMHIVGIVEIVAGFIVLSRWTKIGSAVVGLWLLAIAVNLVTTGMFY
                     DLAVRDVEIAIGAFALFRITECRERAQAESATARSFETRAIAS"
     gene            11648..11980
                     /locus_tag="VHE33_10965"
     CDS             11648..11980
                     /locus_tag="VHE33_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98017.1"
                     /translation="MKNVIRLALIALLALPAGAETFKNVSVVDNNCSRRVASNPDSHT
                     KECALKCAASGFGIITADNQYLKFDEAGNQKVLSEIKSSSKADHLRVDVSGTVRGDTI
                     QVSSVKVL"
BASE COUNT         2410 a         3845 c         3545 g         2395 t
ORIGIN      
        1 gggcgctctg aaaatcaggc gaagagaatt tggtgcggaa ggggggactt gaacccccat
       61 gccttttggg cgccaccccc tcaagatggt gtgtctgcca atttcaccac ttccgcactt
      121 aaaaactgct ttgttttcaa cttgttgacc ggaaccgttg ttgagggccg attccagtgt
      181 attcattttt gtcactaccc tgcgcctcgt gcctcggtgt gcctgccggc ggttctcaag
      241 tccgcccagt cgcatgaaca acgcctgtac ggcgttggcc tgcggatgca cgtagcgcct
      301 cgtcgtttta atgttatcgt ggcccgccac gtactgcaac gtaaacgggt ctgtgggctc
      361 gatcgtgcca ggaacgcttg ccgcgtggat tcgaacggcg gcacacggaa tactcctgga
      421 accgcccaaa cggcaaaccc tgaatgtccg ccgcttgtca gtcctgtggc agcggccgac
      481 aataacatgc agcgatgcaa cttcctctgc gcagattgct gttgatcatc ggcctctgcg
      541 tcgcgtccac gtggacggct gaaggccagt ccacgcccgg cgttcaggat ggcgttgccc
      601 tgatgcagca ggggcgcctc gaagaggcgc gcgccatctt ccaatcggtt ctgcagcgcg
      661 atccccaaaa ccagcaagcc caggatcagg cggttgctgc cacagagcgt ctggccctgg
      721 agcagagagc cggcaatcat actgtggacg cgctccagga attgatggat ggccaaaaat
      781 tagtgccgtc cagtgcgcgc cttgcctatg acatcggcat ccttgaagac gagatgcagt
      841 tgtatccgga ggcggcgcga gcgctggaaa ccgcaaagca gctccatatg gacgatccca
      901 tgctgctcta tgcgatggcg cgggtgcaga tggatgcagg gcagctggaa acggccgcac
      961 agaccatgca gcaatacctg caggtgcggc ccggcgatgc cagcgcccac ttcgggctgg
     1021 gccggattta tcagatcgga atgaagttcg acaaggctgt gccggagttt caggaatcca
     1081 tccgtctgca gccggtgcaa agcgaggcct ggtaccaact cggagacatc gcactgaagc
     1141 agcatgagtt tgatgaagct ctgacggact tcaaccacac actcgaacgc aatccccgcc
     1201 atggtggcgc gctggccggc tccggagaat gcctcttccg ggaaaagcga tatgaaaagg
     1261 cgctgaagtt tcttgaccgc gccattgcgg ccgcgcccga ttatcagaca ggccactact
     1321 atcgcggact gacgctggcc agactcggac ggaaagaaga atccgagaag gagctggcta
     1381 cagccacggc gcttgcggat gcggaaaaca agaaatcggc tacgcgctat cagctcaccg
     1441 tgaccgggca gccttagtgc agtgacgcca gcgaaccgcg cggccgacgg cttttctata
     1501 accctcgacc agcggcacgg cccacagagt gcgccggcgc atcgcccacg ccctgttttt
     1561 cgctccagta tcaatcgcgg ctgtgtttgc cgtagagcgt ttcgcaaagt gcgaactcag
     1621 cccgtgcttt gtccgcttca cccaggtttc gatacgcctg acccagctcg aaatgggcct
     1681 tgggatcttt cggatcgagc cggacggctg cctccagctc gctcgcgcct gcctttgcat
     1741 cgccggttaa cgacagcgcg cgccccagtt tttcgcgggc ttccgccaga tccggcgcca
     1801 gtgccgccgc tgttttcagg gatggcacag cctccgcggc gcgctgctgc tcgaggagaa
     1861 aaactcccag gtcgagaaac ggccatggat cggtagtctt tcgttccgtg gcccactgcg
     1921 ctgcctgccg cagcgccgcc tcggccttgt ccgtttggtt ctcggcgtcc agcgtcaggc
     1981 ccaggttctc ctctgccttc agatgttcgg gccggagggt cagaacccgc cgaaaggcca
     2041 gttctgcctg gtcgaagtga ttctgcgtgt agagacagcg cccgaggctg tacaggacgt
     2101 ccgcattgga ggggtcggtg gagaacgcga tgcgcagcca gtgcaccgcg tcgtcatagt
     2161 cgttcaggag cacgtaatcc agagccacgg aacgcagctc attcgcggtt gggcggcgaa
     2221 gcgcggccgc ctgcgtgtac agagcaagcg actccttcgg cttcacctcc tgccgcagga
     2281 ccagcgcgag atcgtaaaga gcttccacag acttcggatc gtttcgtaag gcatcgcgga
     2341 gcctggcctc tgtgacctgc aggctcggtg agatcgcagg ttcctctgcc gtcaggggca
     2401 atgctggacg gcgctcggct ttatcgggat tcagttgctg ggcgattgcg catgagccgg
     2461 aaagcacggc agcgagaatc ggagaaatcc agaagcgcag tcgcacgtcg tttcagtccc
     2521 ggcacgctcg atggagcgca cacgttcagc gtactcccca aaaccatgcc gcggagaggt
     2581 gactctcccg ttcggcccgg tttggtgtgg cgtttctgaa ttttcttcac cttgtcatcc
     2641 cgcgttgtgg gtgaagcggc cgcaggcttc cggccctatc gcggcgcgac tcccgccgcc
     2701 tgcaaacggt ccagttccgc cttcgcgcgg cgcacatccg cttcgcgatt cagtctgcga
     2761 tagatgggta acagcgcata ccacgcagcg cgctcgatga agggcgaggg atgctgcttc
     2821 aggcacgtct ccaatgcgcc gacagcctcc tggtcgtgtc cggttttgcc gaggccgaca
     2881 cccagaaggt agtacgcctg gaccggatcg gcatgcgcat cgatcgcctc gcgcagcaag
     2941 gggattccag actcagggtc cgccccttca acgtcgattc gaccgagttg cagcagcgcg
     3001 gtcgtgctgt gcggattcag ctccagcgcg cgcccgtagg cttgggcagc ctcatcgagt
     3061 ttcccgctcc gctgatactc cacgcccagc gcatcgtaaa gatccgcgtt gttcggctga
     3121 tctttgacgg caatcgcgta ttcctcagcc gcccgctccg gctgcccgcc cgccgaatag
     3181 atctcggcca gagctcgatg aacatgccac gattttggat cgagcgcgta gagctgctga
     3241 aagcactccc tcgcgacctg cgtgtgcgcg cgtgcccgca gatcgaggat attggcatcc
     3301 tttggcgcga gagccccggc ccggtccagc ggtccgactg ctgcactcgc atcccccctg
     3361 gccatctcaa cgatgcccag ccagctcagc acttctgtgt tgttcggttg gatttggagt
     3421 tcggtcttca gcgagtcgac agcagcatcg aactgattct gctgatactg tgcgatggag
     3481 agaaataaat gcgcccccgg cagatcagcc ctgagccgaa ttgcgtgctc caggggctct
     3541 tctgctttgt cgccgtgccc ctctcgcaat aacgtcatgc caagaccaag gtaggcattg
     3601 ggcgaatcgg ggtaggcacg gaccgcgtca ccgaagcgca gctcggcgtc ggcgaaccgc
     3661 ccttcgtgaa tggcctgagc accctgccgc agcaactcgg cctcggatcc gggaggcgtc
     3721 tgcgtcccgg ccgacatggc aggcaggcac tgaagcattg ccgccgacca caaggcaacg
     3781 aaacaaagca gcgttttcgc catcgccagc tcccaggggt gggatgcgga acgtcgctca
     3841 tcataaacct ggagtcgcgc ggcatgcaga gtgcgaagat cactccgatc gcgcgcgtgc
     3901 tgcctggttt gtggtagcga gaaaacgttc tccagcattc tccccggagc gtgcgccgga
     3961 aaatccctcg caccggcgga tccactgcgc ggcgcggaat tgaaatagta ctgtcagaac
     4021 agttaaatgc acaggtcagc cctgctccat cgtagccagc cgacgcctgc ccgatgattg
     4081 gagaaaaggt attgcgaaag gagcgacgcg gaatctcgta cgaacgattt cgagcgggtc
     4141 tctttcgatc ggcaccggca cgaccgattc catctccgaa cggtttctta ctccaagggg
     4201 aatagctttg tcgtaataaa gtcacaattg gcttgacacg ctgacagacc gcttcttagc
     4261 atggctggtg tttaatgacg gccgaagcgc gcttcagaaa acgattactc attaaaaaga
     4321 tcgcgcaact gaaacaccgt ggtcgcagaa ctcttgactc gaccaccttt gctcacatgg
     4381 aggccttacc gtgacccgaa aaccgcgcat agccggtttc ttccgcatca tgggcgtttt
     4441 gctcacgttg ctttccgttc ctctcgttgg tttcgcccag tccgcacgcg gttcgctcgc
     4501 cggcacggta accgatccat ccggagccgt catcgtcggg gccagcgttg ctgcgactca
     4561 aaatgaaacc ggttccaaag cggaaaccct gaccaccagc tcgggcgact tccgttttcc
     4621 cgaactgccg ctcggtcgtt acgacatcgt tgtaaccgct gcaggctttt cgaagtccac
     4681 cagcacgggc gtactcatta ccgtgggcag catttccacg ctgaacgtcg tgctgaaaac
     4741 cggctcggtc gccgaaacag tgaccgtcga cgccagcgcg ccgacccttg agaccgcaag
     4801 ctccgacatc ggcggtacgg tcagcaccaa acagattgaa gacctgccgc tgtccctggc
     4861 gctcggcgtc aacggcatgc gctctcctga gacctttgag ttcctcgttc ccgggaccac
     4921 cggccccggt tcggctaccc agagcaattc atccaacggc gttttcttcg cccgtatttc
     4981 cggcggccag gcctacggca acgaaacact gctcgacggc gccagcgtgc agcgcagcga
     5041 gaacggatcc tcgttcgatg aaacctcgcc gtcgatcgag gcgctgcagg agttcaaagt
     5101 cacgacctcc accccatcgg ccgagttcgg ccgcaccact tccggcatta caagtttctc
     5161 caccaagtca ggaaccaacg attttcacgg gatcggctac gccatcgtga agaaccgggc
     5221 cttcgatgca aactcctggt tcaacgacgg ctacaaggcc taccaatgcg tcggcgtctc
     5281 cgacctcaat tgcgcttata gcaagcccgc cgacagcaag tatgacttcg gcggtatctt
     5341 cagcggcccg gtcaaaattc ctcatctcta cgacggaagg aaccgctcgt tctttctgtt
     5401 tgcgtgggag caatataagt tcaagctggg aggcacgcaa tatgcgacgg tgcccaccgc
     5461 tgcggagcgg accggagact tcagcgccga tctcggaccg gacatccccg gaggctcgcc
     5521 ctatccggga gccccttata acgtgcttct gaacccctgc acggggacgc cgctgcagta
     5581 caaccagatc ttcgacccac gaaccagtac ccaaatcagt ccaaacgtgt tctgccgtac
     5641 gccgtacccc ggaaacgtaa tcaactctcc catgagcgcg gctgcccaaa agctgctctc
     5701 cgctttgccc ctcccgaatc aggctcccct gagcaccgac ctgtatggat acaggaacaa
     5761 ctacgtgacc tcgggcacgc gcagcatcgt caacaccacc tataccattc gcatcgatca
     5821 gaatatcggc ctcaagagca agatcttcgc gacctacgac acgcgccaga acacggcgct
     5881 cgaggggatt ccgaactttc ccgagccgta taacagcaac ggctatcccc aggcattcac
     5941 cacacactac tcacgagccg ggtgggacta ttccatttcc ccgaccgtgc tgaatcatct
     6001 gaacctgggc tacaaccgca ccaacagcgt gaacgtgggt gcgacgtacg gcacatccca
     6061 gaccgcctct tctctgggga tcgcgaatga cttctcgaag ttcttcccgc tgattgtttt
     6121 ccccagcccg gatcaaccga ccacctgggg acagcagcag aacggcgaca acatcgacaa
     6181 cggcatacgg gtcaatgaca gtgtgagctg gatcaaaggc cggcagagcc tgaagttcgg
     6241 cgtcgacttc cggaaccagc agtactccgt catccagtac aaccaggaca cgtttaattt
     6301 ttaccgcgat cagaccgccg gcctcagtaa tgcctgctgc gggtcgggaa gctcgatcgc
     6361 cagcttcctc accggtgagg tcggcaacgg cttccaggcc gtctataacg atcacccccg
     6421 ctggaactcg cactacatcg ccggcttctt tgaggatgac ttaaagcttt cgaaaaacct
     6481 gactgtgaac ctgggcctgc gctacgacgt cgatgtgccg cgcaaggaag cgctgaaccg
     6541 cacctcggca ttcagcttca ccgcccccga cgcagccgcc ggcaatctgc cgggcgccct
     6601 ggttttcggt accaactgca gtcattgcaa taccgcctgg gcggacacct ggtggaaaga
     6661 ctggggaccc cgcgttggct tcgcttatgt gctgccacac acggacgata aggtagtcct
     6721 gcgtggtggc ggcgccatcc tctacggccc gctgcagtac agtgatttcg gctcgtcgat
     6781 gtcgctcggc tacaaccaga accgctcgtt ctactcggtg caaaacgcgg cagccaacac
     6841 gggcggcgcg tttacgccgg ccttccggct ggattcaagc tccactgcgg accccaccaa
     6901 tccatcggtt ggctttccgg ctgtgagcta cgcgccaaac ctcgatccca cgcagctgac
     6961 tgcgcaaaac ggtccgggga gcttcgctgc cgtcggcggt gaagtcattc gttctaactg
     7021 gggccgtccg gccatggtcg acaactggag cctgcagctg caggatcagg tggcgcagga
     7081 tctgatcctg accatcgggt atatcggaca atcggggcag aacctgcatt ccggctatct
     7141 gtccaacatc aacaacatcg acccgaaata cttcagcctc ggcgacaagt tgaacatacc
     7201 gggctacacg attcccctcg gtgggtccaa cggcggcgtc gacgcgccgt atgcaagctt
     7261 caccggcgcg ctgggacagg ctttgcggcc cttcccccag tacgattaca tcgccggtga
     7321 ttgctgtctc gaaaacatgg gccactcctc ttacgaagcg ctggtcactt cgctccagcg
     7381 ccgcttccac gatggactga atctccaggc gtcctacacc tggtccaagg cactgacgga
     7441 cgcggattcg acgattccgt tcagctacat taccggcggc ggcggcaaca ccctgcgaca
     7501 gggccagggt tccgccaacc tgcatctgga aaaggcggtg agcgcgcagg acatcaccca
     7561 ccagttctcg ctgagctatc tttaccagct gccctttggc aagggccgca agtggctgca
     7621 caacagccgc gcggctgacc tgctgattgg tggatggcag gtgggcgcga ttcagcgcta
     7681 ccagagcggg gagcccgtca gcttcggctg tgcctccggc attcccttct accagaactg
     7741 cattacctac acggccggac ccgcatcgaa gggcgggacc agtttcgcca gcgcagccta
     7801 caaggcgaat aagaacggcc cgagctactt caaccagaaa tcctggttca aaccggcgta
     7861 caacacgatt gcttcgtcgg ggccgctcgt cgcgctttcc gatgcggcat tcgtggatca
     7921 aaactcggag tttgggccgg accgtacttt cacaccagga tgcgctgcct ttaacggcgg
     7981 caatgcctgt tccttcgatc cgttcagtct tggcaacata ccccgcgtta cgaacaagat
     8041 caccggacca aattacctct ccgaagacgt gagcctgctc aaggattttc atatcactga
     8101 gcggttggtc ttccagctca agggagaagg gttcgatatc ttcaaccggc accggatggc
     8161 gattcctgac acaagtccgg gcgatctctg caacggaacc ggctgcaccg gcttcggtat
     8221 tccaaccgcc accgattatg gtccgcgtaa tctgcaggtc agcggtcgcg tcaccttctg
     8281 ataaccaacc gggctcttct ccaaaagagg agagcccccg cttcgcttgc tggaggtcag
     8341 gcgttctgcc tggccttctt tcttgcattg agccccattc cgctactgaa ttcctgtccg
     8401 atgtatactc tgccgtccgc ctggtgaatt ccgatgatgt cgcgcgtgct tctcatgagc
     8461 ttcatctgcg tcgttggaat ctcatttgtt tcgccgcgat ttgaacgcgc gcagcaatcc
     8521 cagtccaaca gctccggaat ggccgccgat tccgcgcagc cgccgggttt cgacgcgcag
     8581 catcggccca tcaccgccgg gggaacagtc gcttccggtc ccatcgtttt cgaggacatc
     8641 gcggatcagg ccggcctcac gcgctggcgc aacatcacgg gaaccccgca gagacgcctc
     8701 atcatcgaag ccaagggatc cggcgtttgc ctgctcgatt tcgaccgcga cggctggctc
     8761 gatatctatc tcgtcaccgg atccaccttc gacgcactga gcggaaaagg gtcggctcct
     8821 catgcggcgc tctttcgcaa taaccacgat ggaaccttca ccgatgtcac tgcgaaggcc
     8881 ggtgtcgcca acgaccgctg gggtatgggt tgtgcggtcg ccgactacga caacgacggc
     8941 tggcccgatc tctacgtcac caacctcggc ggcaaccgcc tgtttcacaa caaccgcaac
     9001 ggcacgttca ctgatgtcgc cgagcaggcc ggagtggcgc tcacgtcgcc tccaggaagc
     9061 attctgaccg actacaccgg agcgaccttc ggcgactacg acggcgacgg tcgtcttgat
     9121 ctcttcgttg cgggatacat ccagtacgat ttgcgaaacc cgcccgtggg cggatcgtct
     9181 tcggttcgca ctgcgcgatg ccagtatcgc ggcgtggatg tgatgtgcgg accgcgcggg
     9241 ttgcagggtg cgccggatca tctcttccat aacaacggcg acggcacatt cactgatgta
     9301 agccgcaagg ccggcgtcga agacgacgcc ggttactatg gcctcggtgc gctcatcgtt
     9361 gatgtcaatg gcgacggatt gccggacttg gtggtggcca acgattcaac gccgaactat
     9421 ctctacatca ataagggcga tggcatgttt gaaaaccaaa gctatgccag cggtctcgcg
     9481 gtcaacggcg aaggccgcga agtcgcgaac atgggactcg cggcggggga ttacgagaac
     9541 aacggtcaac tcgacctggt cagcacaacg ttttccgatg actacgatgt cgtcttccgc
     9601 aacaacggac gcggagaatt cgaagacgtc agctaccgta cgcgcatcgc gcagccaaca
     9661 ctgccattcg tcggttttgg cgacggcttc ctcgactacg acaacgatgg ctggaaggat
     9721 ttattcattg ccaacggcca cgtttacccc accgccgacc atcatccgga atggggtacg
     9781 agctacgcgc agcgcccatt gctgttccac aatcagcatg acggaacctt tgcgctggtg
     9841 ccggccgtac agggcaccgg cctggcaacg ctcagcgttg cacgcggcgc cgccttcgga
     9901 gacctgttca acgacggcag agtcgacgta gtcatcaaca atctggaggg ccgtccggtc
     9961 ttgttgcgca acgtcaactc cgatcgaaat cactggatcg agttacggct gatcggaggg
    10021 tccagaagcc cgcgcgatgc tgttggggcg acggtttatg tgacggccgg cggcgttcgc
    10081 cagcgggccg acgtcctgag tgggggaagc tacctctcgt ccagcgatct tcgtttgcat
    10141 ttcggcatcg ggacatccac ccatatcgat ggcatcgaga ttcactggcc ggatcgtggg
    10201 atcgagaaga tcgctgtgaa gtctgtcgac cgcatcttca ccctcgtgga gggaaaaggt
    10261 gtgacggagc aaatgccgac ccaactcgct ccccagcccc gaactccggc gccaaacagc
    10321 gcgccgaaaa ggccgggaat ggcgtcctga aaccttcccg tcatcatgcg gattcgggag
    10381 gcgaccggag tcagcgggga cgttgctggt ccgccagccg ggcaaagacg ccggcgacga
    10441 tccggtcgca gcgctcgccc atgaagggga acgcagtctt tgccgacgcg ccgacttttt
    10501 ccatcagggc tttgcgcgcg agggcccgcc cgcgatgcaa acgcactttc acgttctgtt
    10561 cggaaatctc gagcgaagca gccgtttcgg cggtggaaag ctcctcgacg tcgcgcagca
    10621 tgaccacggt gcggtacatc tcgggcagat tcagaagggc ctcttcgagc attccgctta
    10681 cctcgctgtg cgccgtgttt tgttccggat cccgttcggc ggagggcggg tccatctcgc
    10741 cgtcgttttc gtcgatggcg gagaagcgat tgcgtcggcg cagccgggcc agcgcttcgt
    10801 gcacggcgat gcgggtcagc caggtggaga aggcggctcg accttcgaac tgatccaaat
    10861 gctggtacgc acgcacccag gcatcctgca tcacgtcctc ggtctcatcg tcctcgcgca
    10921 ggatggcgcg caccgtgcga tagaggcgtt gattgtagcg gcgcatgagg atctcgtaga
    10981 gcgccgtgtc gccggcgagt acgtggagga cgacctgttc gtcgctccag gcctcgacgc
    11041 gcacctgttc cagcaattcc atcgtccctc cccaatggta tagagaatgc gttcggcaga
    11101 aggttacaga ggtccgcacg aatttctgtt gtaaccttcc gggctcccgc ggctccattg
    11161 cggcaggagg gatcgtatgg agcgcgtcga ttcccgtctc aattcagcct ggtgggcgct
    11221 gcgcctcggg ctggggctgg gtccgattgt ggccggcctc gacaagtatt tcaacaagct
    11281 gacggactgg accatgtacc tgagtccact ggtgaccaaa gtggttcccg tgagcccggc
    11341 gacgttcatg cacatcgtcg ggatcgtgga gattgtcgcc ggattcatcg tactgagccg
    11401 ttggacgaag atcggcagcg ccgtcgtagg actttggctg ctggcgattg cggtgaatct
    11461 ggtcaccacc ggcatgttct acgacctcgc ggtgcgcgac gtggagattg cgattggagc
    11521 ctttgcgctg ttccggatca cggagtgccg ggaacgcgca caggcagagt cagcgactgc
    11581 tcgctcgttc gaaacccgcg cgatcgcttc gtgaactcaa cccctaaccc cagtacggag
    11641 gagaagaatg aagaacgtta tccgccttgc tctgattgcg ctgctggccc tgccggcggg
    11701 cgcagagacc tttaagaacg tgtcggtcgt ggacaacaac tgctctcggc gcgtcgcttc
    11761 caaccccgac agccacacga aagaatgcgc gctgaagtgc gcggcgagcg gcttcgggat
    11821 catcaccgcc gacaatcagt atctgaagtt cgacgaggcg ggcaatcaga aagtgctctc
    11881 cgaaattaag tcgtcgagca aggccgatca cctgcgcgtg gacgtgagtg ggaccgtcag
    11941 gggtgacacg atccaggttt cctccgtcaa ggtgctgtaa gtcactccgc tggctcgggc
    12001 agggttctgt gcccgagcct ttttttgcca cgtggcatga accgagagcc gcttagtgcg
    12061 cgccggccgg cggtcgcgcg cccgggcggg ctttcttgaa gaaccaaacc agcacaacgc
    12121 agacgaagca gatcaggctc atccagcgga agacgtcgac gtaggcccac agcagggcct
    12181 ggcggcccag ctggc
//
LOCUS       DASZYN010000424         3163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_21081, whole genome shotgun sequence.
ACCESSION   DASZYN010000424 DASZYN010000000
VERSION     DASZYN010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21081"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1198..2229)
                     /locus_tag="VHE33_10970"
     CDS             complement(1198..2229)
                     /locus_tag="VHE33_10970"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HVT98018.1"
                     /translation="MNIREVARRAKVSTATVSRTINSSDKVTPETKERVQRAIEELGF
                     YPNTWARALGSGRSNLYGLIISDITNPFFPELVKSFEDIAVQNEQEVLVANKSYDPAR
                     MEGCVRRMLQRKVDGVAIMTSEMDARLITEFQSREIPLVFLDTGKAEKLTSNIVVDYA
                     AGVDAAVEHIVGLGHTDIGFISGPMMLRSARVRREAFIGCLERRGIGADWIEEGNHQV
                     DGGHDAMARLLGRKTRPTAVLASNDMTAIGALGAIHERGLRVPEDISIVGFDDIQISA
                     FTQPALTTVRLSREEIARVAFRALYSIRQDAGKGAEYGVQPALVVRRSTGRAPRGAGA
                     AEREPVLVS"
     gene            2472..>3163
                     /locus_tag="VHE33_10975"
     CDS             2472..>3163
                     /locus_tag="VHE33_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98019.1"
                     /translation="MQPALLRSIARPIAAALFLCLALSAAAQKSPRWTEQQANTWYAH
                     QPWLVGSDYLPENAINQLEMWQADTFDPQRIDLELGWAEAIGMNTMRVFLHDLLWQQD
                     PDGFRKRIDTFLALCAKHHIRPILVFFDSCWDPNPKLGPQHPPIPGVHNSGWVQSPGR
                     AALADPSQYPRLEAYVKGVIAAFASDPRILAWDLWNEPDNGNESSYSNGELTSKQALI
                     AKLLPQVFEWARS"
BASE COUNT          604 a         1184 c          739 g          636 t
ORIGIN      
        1 ctgggctggc ttcgacaatc ttcgcttcgc cgtcctcaac gccctcgccc gcgccctcga
       61 actcaagcgc ctgcacccct cgggagaggt gcagtaaacg aatttgcgga aaagattgcc
      121 tggcgcagaa gcgcgcaggg aacgagcagc gaagagctgt cctgacccac gccccgaaac
      181 tagcgagtgt gggtttctga tgtttcttca ccttgtcatc cgcccggacc cttcgcgcaa
      241 cgaaggggaa gaggagggac ctgccctttc ttcatcttgt tatcccgacc ggaccctgag
      301 catagcgaag gggaagcgga gggacctgct tctctttcgc ataaagaaat tccggaaaca
      361 ccacgctcgt gtggcgtttc tcaagtttct tccccttgtc atcccgccgg accctgagca
      421 tagccaacgg agagcggaga gacctgcttc tctttcgcat taagaaattt cggaagcatc
      481 acgctggtgt ggtgtcccgc aaattccttc aacatccgat cctggttttg tatcagggcg
      541 cgacttcaca gcttgcgaaa aacccgaagc tttgaaaggg tccggcttga gccggcggta
      601 acgcccccaa aatcaatccg gcttcagcca ccgagggagg agttctgctt tctgcagact
      661 tctccgcaag ctttttcagt cgtgccacaa ggctcccaaa acgcatccgg gctttagccc
      721 ctgaggggct cttcctcccc ggctcgcttt tttccgcaag accttcagtc gtgccataaa
      781 gaacgcaaaa ccaggggggc ttcagtccct gcgccctgcc ggtcgggtgc ctgcggtcgc
      841 gtgcctgatt gttcaatgaa cttcagggac acaacacttc cggtgcatcg gtggggatgg
      901 ctgcgccgac acgttagcct aaggttcatt cgagaatctg cagcaggtca ggcgagaatc
      961 tgcagccggc cttaactccg cccaaaccag gcaaacggcc cacccgccca aaagcatagt
     1021 ttggccaggt tcaccccaca gcaagaagcg gactcctttt gcgcgcaaga atcaatgcca
     1081 acccgccctt gctgcgcacc aaagcaccgt ccccgcacta cccaaaacca aattgggaaa
     1141 atgtaacctg ttcatcttcg ccgggccgca gcacgctccg aatcaggcga ctgcccctca
     1201 gctcaccaga accggctccc gctccgccgc tcccgcacct cgcggcgcac gccccgtcga
     1261 ccgccgcacc accagcgccg gttgcactcc atattcggcg cccttgcccg catcctggcg
     1321 aatgctgtac agcgcccgaa acgctacccg cgcgatctcc tctcgcgaca atcgcaccgt
     1381 cgtcagcgcc ggctgcgtga aggcgctgat ttgaatatcg tcgaacccca caatcgaaat
     1441 atcttccggc acccgtaatc cccgctcgtg gatcgccccc agcgccccga tcgccgtcat
     1501 atcgttcgac gccagaaccg ccgtcggccg cgtcttccgc cccagcagcc gcgccatcgc
     1561 atcatgcccg ccatccacct ggtggttgcc ttcttcgatc cagtccgccc cgatcccccg
     1621 ccgctccagg catccgatga atgcctcccg ccgcacccgc gccgatcgca gcatcatcgg
     1681 ccccgagatg aatccgatat ccgtgtgtcc cagtccgaca atatgttcca ccgccgcgtc
     1741 cacacccgcc gcataatcca ccacgatgtt gctcgtcagc ttctccgcct tgcccgtatc
     1801 gagaaacacc agcggaatct cccggctctg gaactccgtg atcagccgcg catccatctc
     1861 cgatgtcatg atcgccacgc cgtccacttt gcgctgcagc atccgtcgca cgcacccctc
     1921 catgcgcgcc ggatcgtagc tcttattcgc caccagcact tcctgctcgt tctgcaccgc
     1981 gatgtcttcg aagctcttca ccagctccgg aaaaaacggg ttcgtgatgt cggaaataat
     2041 cagaccgtaa agattgctgc gccccgaccc cagcgcccgc gcccacgtgt tcggatagaa
     2101 ccccagctcc tcgatcgccc gctgcacgcg ctccttcgtc tccggcgtca ccttgtccga
     2161 gctgttgatc gttcgcgaaa ccgttgccgt ggagaccttc gcccggcgcg ctacttctct
     2221 gatgttcata agcgatctga ccccttatat tccatccgcg cacacttaag aaagatcatt
     2281 cgcagaaagc gtttacgcga atccctcccg ttctcgacct tcactcgtat ttcggctcgc
     2341 acttcgctcg cacttcgctc gcattttcca ctcgcagttc gctcgctttc aactcgcctt
     2401 tcgctcattt tttactcgca gttcgttcgc ttttcgctct cctcccagcc tttcctgtac
     2461 gatgaccgca aatgcaaccg gccctccttc gttccatcgc gcgacccatc gccgccgctc
     2521 tcttcctctg cctcgccctc tccgctgccg cccaaaaatc tccccgctgg accgagcaac
     2581 aagccaacac ctggtacgcc caccagccct ggctcgtcgg cagcgactac ctcccggaga
     2641 acgccatcaa tcagctcgag atgtggcagg ccgacacctt cgaccctcag cgtatcgacc
     2701 tcgaactcgg ctgggccgaa gccatcggca tgaacaccat gcgcgtcttc ctccacgacc
     2761 tgctctggca gcaggatccc gacggcttcc gcaaacgcat cgataccttc ctcgccctct
     2821 gcgccaaaca tcacatccgc cccatcctcg tcttcttcga ctcctgttgg gatcccaacc
     2881 ccaaactcgg cccccaacat ccgcccatcc ccggcgtgca caattcagga tgggtgcaga
     2941 gccccggccg cgccgccctc gccgacccct cccagtaccc gcgcctcgaa gcctatgtca
     3001 aaggcgtcat cgccgccttc gcctccgatc cccgcatcct cgcctgggac ctctggaacg
     3061 agcccgacaa cggcaacgaa tcctcctact ccaacggcga actcaccagc aaacaggcgc
     3121 tcatcgccaa acttctccct caggtcttcg aatgggcccg ctc
//
LOCUS       DASZYN010000425         2434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1238805, whole genome shotgun sequence.
ACCESSION   DASZYN010000425 DASZYN010000000
VERSION     DASZYN010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238805"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2434
                     /locus_tag="VHE33_10980"
     CDS             <1..>2434
                     /locus_tag="VHE33_10980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HVT98020.1"
                     /translation="TDLGGTPSLRALLARVRKVTLSAFDHQELSFEQIVEDLQPERSL
                     SHSPIFQVLFTLHNTPVVEQKIGDLLISPLEVEHTTSKYDLSLDLGKTEAGELEGFFE
                     YNSDLFEAETIARLARHFTRVLEEMARDIEQPINAFVLLTEDEVQRQTVTWNTVEAPY
                     ELNEGYVERFRRQAALTPQAVALVCGTDSLTYRELDRRSNALALRLRARGVGPERLVA
                     VLDQRDLPLAVALLAILKAGGAYLPLDPSQPLSRLRQMIRQSGCAFVLTGEPFAARLQ
                     SVLDDFPAGERPAWEVLNATEEAAEGPELPWQGERLAYVIYTSGSTGLPKGVMVEHRG
                     MLNHLLAKVAALDLRPSDCVAQNASQCFDISIWQLLAAWLVGARVHLFPDQQAHQPAE
                     LLAEVQRGGVTVLELVPSLLRALLDLLEQRQVAPPAALRWLIATGEALPADLCQRWLA
                     LAPQIPLLNAYGPTECSDDVTHMEITQADELSGPVAPLGYPLPNTRLYVLDAAQQLVP
                     VGVSGELWVGGVGVGRGYLNDSRRTAEAFRPDPFGAPGQRLYRTGDLVRARPDGQMEF
                     LGRIDQQVKLRGYRIELGEIEAALRKHPSVRDCLVILRESETHNARLLAYVVPHSEAA
                     VSPADLSAHLKNMLPEYMLPAGYLLLDALPLTANGKVDRKALPEPEYQFAQEDTPYVA
                     PSTPTEQTLAAIWSSVLEYPRVGIHDDFFLLGGHSLLAIRLMSQIEQQFARHLPLEMI
                     FQWRTIAELASVLDRVTAQSAWETTSDPAWEELNLYTEATLATSDCPEVWPQDLASEP
                     TTILLTGATSFLG"
BASE COUNT          413 a          782 c          782 g          457 t
ORIGIN      
        1 caccgacctg ggcgggacac cgagcctgcg cgccctgctg gccagggtac gcaaggtgac
       61 gctgagcgcg ttcgaccacc aggagctttc ctttgagcaa atcgtggaag atctgcaacc
      121 ggaacgcagt ctgagccatt cgccgatctt ccaggtgtta tttacgctgc acaatacgcc
      181 ggtggtcgaa cagaaaatcg gcgatctgct gatttcaccg ctggaggtgg agcacactac
      241 gtccaagtat gatctgtcac tggacctggg caagaccgag gcaggcgagc tggaaggctt
      301 tttcgagtat aacagcgatc tgtttgaggc ggagacgatt gcgcgcctgg cccgccattt
      361 tacgcgggtg ctggaagaaa tggcgcggga catcgagcag ccgatcaatg cgtttgtgct
      421 gctgaccgag gacgaggtac agcggcagac tgtgacctgg aatacggtcg aggcgcccta
      481 tgagctgaac gagggctacg tggagcgctt ccggcggcag gcggcgctga ctccccaggc
      541 cgtggcgctg gtctgcggca ccgactcact gacctatcgc gaactggatc gacgctccaa
      601 tgccctggct ctgcgtctgc gggcacgcgg cgttggacct gagcgcctgg ttgcggtact
      661 cgatcagcgc gatctgcccc tggcggtggc cttgctggcc atcctgaaag ccgggggagc
      721 ctatctgccg ctcgatccct cccaaccgct gagccgcctg cgccagatga tccgccagag
      781 cggctgcgct ttcgtgctga ccggcgaacc tttcgccgct cgcctgcaaa gcgtgctgga
      841 tgacttcccc gccggggagc ggcccgcctg ggaggtgctg aacgcgacgg aggaggccgc
      901 cgagggaccg gaactgccct ggcaggggga acggctggcc tatgtgatct atacctctgg
      961 ctcgaccggt ctgcccaagg gagtgatggt cgaacaccgg ggtatgctca accacctgct
     1021 ggccaaggtc gccgccctgg atctgcgccc ctccgattgc gtggcgcaga atgcctccca
     1081 gtgcttcgac atctccatct ggcaactgct ggccgcctgg ctggttggcg ctcgcgtcca
     1141 tctcttcccc gatcagcagg cccatcagcc cgctgaactg ctggccgagg tccagcgcgg
     1201 cggcgtgacg gtcctcgaac tggtgccctc gctcctgcgc gctctgctcg atctgctgga
     1261 acagaggcag gtggctcctc ccgctgcgtt gcgctggttg atcgccaccg gggaagcgct
     1321 gcccgctgat ctctgtcagc gctggctggc cctcgctccg cagatccctc tgctcaacgc
     1381 ctatggtccc accgagtgct ccgacgatgt aacccatatg gagattacgc aggcggacga
     1441 gttgagcgga ccggtggcgc cgctggggta tccgctgccc aacacgcggc tgtacgtgct
     1501 ggatgccgct caacaactgg tacccgtggg cgtcagtggc gaactgtggg tcggcggcgt
     1561 gggcgtggga cgcggctacc tcaacgattc tcgccgcacc gccgaagcgt ttcgtcccga
     1621 tcccttcggt gcgccgggac agcgcctcta ccgcaccggc gacctggtgc gcgcccgccc
     1681 cgatggtcag atggagttcc tgggccgcat cgatcagcag gtcaagctgc gcggctatcg
     1741 catcgaactg ggcgagatcg aggccgcgct gcgcaaacat ccctcggtgc gcgattgcct
     1801 ggtcatcctg cgcgagagtg agacgcacaa cgcgcgcctg ctggcctatg tggtgccgca
     1861 cagcgaagcg gcggtgagtc cggcggacct gagcgcgcat ctcaaaaata tgttgccgga
     1921 atatatgctg cccgctggct acctgctgct ggacgcgctg ccactgacgg ccaatggcaa
     1981 ggttgatcgc aaggctctgc cggagccaga gtaccagttt gctcaggagg atacgcccta
     2041 tgtcgcgccc tctacgccga ccgaacagac cctggccgct atctggagtt cggtgctgga
     2101 gtatccgcgc gtgggcattc acgacgactt ctttctgctg ggaggtcatt cgctgctggc
     2161 catccgcctg atgagccaga ttgagcagca gttcgcgcgc catctgccgc tggaaatgat
     2221 ctttcaatgg cgaactatcg ctgaactggc cagcgtgcta gatcgcgtta ccgctcagtc
     2281 ggcctgggaa accaccagcg atcccgcctg ggaggagctt aacctttaca cagaggccac
     2341 gctggccacc agcgattgcc cggaggtctg gccgcaagat ctggcctcgg agccgactac
     2401 aatcctgctc accggcgcga caagctttct cggt
//
LOCUS       DASZYN010000426         5217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_532859, whole genome shotgun sequence.
ACCESSION   DASZYN010000426 DASZYN010000000
VERSION     DASZYN010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532859"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VHE33_10985"
     CDS             <1..922
                     /locus_tag="VHE33_10985"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98021.1"
                     /translation="YLEKLIERPRHIEIQIIADEHGHCVSLGERECSVQRRHQKVIEE
                     APSPVVDPDLRRRMGEAAVRLARSAGYTNAGTIEFLVDDQQNFYFLEMNTRLQVEHPV
                     TELVTGYDLVHLQIRIAQGEPLPFTQSDVHLRGHAIECRVYAEDPAHGFMPSPGRITR
                     LLQPGGPGIREDSGIYEGWVVPVDYDPMLSKLIAYAPTRDLAIARLLRALDEYVIGGI
                     RTNLGLFRRILTDADFRAARIDTGYLDRLLAATPPPPPAAGNGHSTIPAVAAALFEAG
                     KPSPNGAFMNGKPASAWKTAARRDAVRLAD"
     gene            936..1436
                     /locus_tag="VHE33_10990"
     CDS             936..1436
                     /locus_tag="VHE33_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HVT98022.1"
                     /translation="MTLHLEIGGRTCQVEVDPADTPGQWRIRVDGQPVEADAHLIRPG
                     VLSLLIAGQSFRVVLDPNTADPALHIGHERIPYRVDDPRSLRSRRRLGGVEGPVTLKA
                     SMPGRVVRVLVEKGDTVAAHQGVLVVEAMKMQNEMQSPRDGRVRELRVAPGDTVSAGD
                     ILAIIE"
     gene            complement(1477..2079)
                     /locus_tag="VHE33_10995"
     CDS             complement(1477..2079)
                     /locus_tag="VHE33_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT98023.1"
                     /translation="MPGKPRRASRRADIVKAAETLLRKRGLNGVTTRAIAESVPCSEG
                     AIYVHFEDRLSLVLEVLREALPEMVVPLHALRKKVGTATPEENLLVAVTGLTRFHRRV
                     TPMLCSLFSQPDLLQRFRASLDGGGKGPHRGIATLAAYIEEEQQLGRVDRQVDAAAAG
                     MTLMAASFFEAFTEALLGSPARPEARHLVQMVLGTRRGTK"
     gene            2185..2616
                     /locus_tag="VHE33_11000"
     CDS             2185..2616
                     /locus_tag="VHE33_11000"
                     /inference="COORDINATES: protein motif:HMM:NF042724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6640 family protein"
                     /protein_id="HVT98024.1"
                     /translation="MTRAHLGKVLLTLVLLGGAVMSFALDWSGNHLLNPLWHPHARYH
                     SAVLLFLFAGAASTATWLLWRRSKEPAVAFTAASLLSLAYWTPFFYVPFLLPHSSYWA
                     GIPGHEPRIAGVTIYPNLVVVALFVVLTLIAWWLGQTAQES"
     gene            complement(2659..3636)
                     /locus_tag="VHE33_11005"
     CDS             complement(2659..3636)
                     /locus_tag="VHE33_11005"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HVT98025.1"
                     /translation="MHYYFVRHLTKFIYSRTVSESTTEVRMHPRSDSNQRCLSFTLSV
                     SPRCRVFTYRDHLGNNIHHFDIPGRHGQMVIVAEAVVEHQPLAAIPTELEPGAWKELD
                     ELVAEGDYWEMLLPSEFAAPTPLLLGLAKELEVTRRDDPLKLLHEINARLYDAFSYEK
                     RSTQVDSPIDEALKTRRGVCQDFAHIMIALVRHVGIPCRYISGYLYHGSEDHDRSTSN
                     ATHAWVEAFLPALGWVGFDPTNALVARDRHIRTALGRDYADVPPTRGLFRGRTDSDLY
                     VAVQVTASDAAPDLERELPIPEDWSMLVEKAQAPPPPAEPLLQIQQSQQ"
     gene            complement(3660..4649)
                     /locus_tag="VHE33_11010"
     CDS             complement(3660..4649)
                     /locus_tag="VHE33_11010"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HVT98026.1"
                     /translation="MLSRVADSLCWTSRYLERAEHTARLIDINMGLMLDRSRVSAERR
                     WQRVLAAVGAAPGLGWQEGDGSEPTRWVGVTALVHALCFDTTRHASITACIFAARENA
                     RQIRDEISTEQWQRLNGLFHEIGRLRTAAPSERNLTEFLPMIIDGVHLFQGVTDTTLS
                     HGEGWQVLRVGRYLERAWSLTMLLDVYEREVFPGEDSDDASQYLEWVGLLRICTAFEA
                     FCRVHTSDLTRERILEFLLLDPAFPHSVRYAVDALHNALEAIQQHTRTQHGGELMRVA
                     GRLKATLGFAEIGEILARDPVRYLRSILDQCKEIHDLIYAVYIQYSVETALSV"
     gene            complement(4702..>5217)
                     /locus_tag="VHE33_11015"
     CDS             complement(4702..>5217)
                     /locus_tag="VHE33_11015"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HVT98027.1"
                     /translation="GNVTLANAFGTGVADDKAIYAYVPAIIQYYLGEEPILNNVETYL
                     LTDAKQRGYVKENLEKLVVKAVGESGGYGMLVGPQSSSAEREEFRRRIDADPRNYIAQ
                     PTLSFSRAPCLLEDGSIGPRHVDLRPYILFGDKVTIVPGGLTRVALRQGSLVVNSSQG
                     GGSKDTWVLGS"
BASE COUNT          954 a         1933 c         1322 g         1008 t
ORIGIN      
        1 ctatctggag aaactcatcg agcggcctcg ccacatcgag attcagatca ttgccgacga
       61 gcacgggcac tgcgtctccc ttggcgagcg cgagtgctcc gtccagcgcc ggcatcagaa
      121 ggtcattgaa gaagcgccat ccccggtagt cgatcccgac cttcgtcgcc gcatgggcga
      181 agccgccgtc cgcctcgcgc gttccgccgg atacacgaac gccggcacca tcgagtttct
      241 cgtcgacgat cagcagaact tctacttcct cgagatgaac actcgtctcc aggtcgagca
      301 tcccgtcacc gaacttgtca ccggatacga cctggtccac ctccagatac gcatcgccca
      361 gggtgagcct ttacccttta cccagagcga cgttcacctc cgcggtcacg ccatcgagtg
      421 ccgcgtctac gccgaagatc ccgcccacgg tttcatgccc tcgcccggtc gcattacgcg
      481 cctcctccag cccggcggcc ccggcattcg agaagactcc ggcatttatg aaggctgggt
      541 tgtccctgtt gactacgacc ccatgctctc caaactcatt gcttacgcgc ccacccgcga
      601 tctcgccatc gcccgcctcc tgcgcgctct cgatgaatac gtaatcggtg gcatccgcac
      661 caatctcggc ctcttccgcc gcatcctcac cgacgctgac ttccgcgcag cacgcatcga
      721 taccggctat ctcgaccgcc tccttgccgc cacgcctccc ccgccaccgg cagccgggaa
      781 cggccatagc acgattccag ctgtggccgc ggctctcttt gaagccggca aaccctcccc
      841 caatggcgcg ttcatgaacg gcaagcccgc gagcgcctgg aagaccgccg cccgtcgcga
      901 cgcagtccgc ctcgccgact aaaggacgcc gccccatgac gctccacctc gaaatcggcg
      961 gccgcacctg tcaggtcgaa gtcgatcccg ccgacactcc aggccagtgg cgcatccgcg
     1021 tcgatggcca gccggttgag gctgacgcac atctcatccg gccaggcgtt ctctccctcc
     1081 ttatcgccgg acagtcgttt cgcgtcgtac tcgaccccaa caccgctgat cctgcccttc
     1141 acatcggcca cgagcgcatt ccctaccgcg tcgacgatcc gcgctctctc cgctcccgtc
     1201 gccgcctcgg tggagtcgag ggtcccgtca ccctcaaagc ctccatgccc ggtcgcgtcg
     1261 tgcgcgtgct ggtcgaaaag ggcgacaccg ttgccgccca tcagggcgtc ctcgttgtcg
     1321 aggccatgaa gatgcaaaac gaaatgcagt ctccccgcga tggacgcgtc cgcgagctcc
     1381 gcgtcgctcc cggcgatacc gtctccgcag gcgacatcct cgccatcatc gaatagccct
     1441 gcgccctgac actccacagg cgacagtccg gtctcttcat ttggttccgc gtcgcgttcc
     1501 cagcaccatc tgcacaaggt gccgtgcttc cggccgtgca ggactgccca gaagagcctc
     1561 ggtgaacgcc tcaaaaaacg aagccgccat cagagtcatc ccagccgccg ccgcatccac
     1621 ctgcctgtcg actcggccca gctgctgctc ttcttcgatg tatgcagcca gggtcgcaat
     1681 cccgcgatgc ggaccctttc ccccgccatc cagtgacgct cggaagcgct gcagcagatc
     1741 gggttgggaa aacagcgagc aaagcatcgg cgtcacccgg cgatggaagc gcgtcagtcc
     1801 ggtcaccgcc acaagcagat tctcttccgg agttgccgtc ccgactttct tccggagcgc
     1861 gtgcagcggc acgaccatct cgggcagcgc ttcacgcagg acctcgagga ccagcgagag
     1921 ccggtcctcg aaatgtacgt agatcgcccc ttcagagcag ggaaccgact cggcgatggc
     1981 ccgggtcgtc acgccattca gcccccgctt gcgcagcagt gtttcggcgg cttttacgat
     2041 gtcggctctg cggctggcgc ggcgtggttt tcccggcatg ttttcgattg tctcgtattc
     2101 gccgcaggct cgatggattg cccataggaa tctttcgatg ctatagtgag cgctcactca
     2161 tcacatgcaa taagggagaa taaaatgacg cgtgcccatc ttggaaaagt gctgctgacc
     2221 cttgtcctgc tcggaggagc agtcatgtcg tttgccctgg actggagcgg aaaccacctc
     2281 ctcaatccgc tctggcatcc gcacgcccgc taccactccg ccgttctcct gtttctcttc
     2341 gccggcgccg cctccaccgc aacatggctc ctctggcgtc gatcgaagga acccgcggtc
     2401 gccttcaccg cagcctccct gctttcactg gcctactgga cgccgttctt ttacgtgcct
     2461 tttctcctgc cccactcgtc ttactgggcg ggcattcccg gccatgagcc gcgcattgct
     2521 ggcgtcacga tctaccctaa cctcgtggtc gtcgcactct ttgtcgtgct cactctgatc
     2581 gcatggtggc tcgggcagac tgcacaggag tcatagccca ctcgatgacg ggccgcttcc
     2641 tgcgacctgg acccggcctc attgctgtga ctgctgaatc tgtaacagtg gctctgccgg
     2701 cggcggaggc gcctgcgcct tctccaccag catcgaccag tcttctggaa tcggcagctc
     2761 ccgctccaga tccggagccg catccgacgc agtcacctgc accgccacat aaaggtcgct
     2821 gtccgttctg ccccgaaata accctcgcgt aggcggaacg tccgcatagt ctcgccccag
     2881 cgctgttcgt atatgccggt cccgtgcgac cagcgcattg gtcggatcga accctaccca
     2941 ccccagcgcc ggcagaaatg cctccaccca cgcatgcgtc gcgttcgacg tcgaccggtc
     3001 gtgatcctca ctgccgtggt acagataccc gcttatatac cggcacggaa tccccacatg
     3061 ccgcaccagc gcaatcatga tgtgcgcaaa atcctggcac actccccgcc gcgtcttcag
     3121 cgcctcgtcg atcggtgaat ctacctgtgt gctccgcttc tcatagctga atgcgtcgta
     3181 cagtcgcgcg ttgatctcgt gcagcagctt cagcggatcg tcgcgccgcg tcacttccag
     3241 ctccttcgcc agccccagca gcagcggggt cggcgccgca aactcgctcg gcagcagcat
     3301 ctcccagtag tcgccctccg ctaccagctc atccagctct ttccacgccc ctggttccag
     3361 ctctgtcgga attgcagcca gcggctggtg ctctaccacc gcctcggcca cgattaccat
     3421 ctgcccgtgc cgccccggaa tatcgaagtg atggatgttg tttcccagat ggtcccgata
     3481 cgtgaacacc cggcaccgcg gactcacgga caacgtgaac gacaaacagc gctggttgct
     3541 gtcgctgcgg ggatgcatgc gcacctccgt cgtgctctcg ctgaccgtgc gcgaataaat
     3601 gaacttcgtc agatggcgaa cgaaatagta gtgcatcgct tcgaatacct cgaatccggc
     3661 tacaccgaca gcgccgtctc cacggaatac tggatgtaaa cggcgtaaat caaatcgtgg
     3721 atctctttgc actgatccag aatgcttcgc aaatatctca ccggatcgcg cgccagaatc
     3781 tctccaatct ccgcaaatcc cagcgtcgcc tttaaccgtc ctgccacccg catcagttcg
     3841 cccccatgct gcgtccgcgt gtgctgctgg atcgcctcca gcgcgttgtg cagcgcatcc
     3901 accgcatacc gcaccgagtg cggaaaagcc ggatcgagca gcaggaactc cagaatcctt
     3961 tcgcgcgtca ggtccgacgt gtggacccgg caaaacgcct cgaatgccgt acagatgcgc
     4021 agcaaaccca cccattccag atactggctc gcatcgtctg aatcctctcc cggaaaaacc
     4081 tccctttcgt agacatccag cagcatcgtc agcgaccacg cgcgctccag gtacctgccc
     4141 acccgcagca cctgccatcc ctcgccgtgg ctcaacgttg tatccgtcac gccctgaaaa
     4201 agatgaaccc cgtcgatgat catcggaaga aactccgtca gattcctctc cgatggagcc
     4261 gccgtccgca gccggccaat ctcgtggaac agcccgttca accgctgcca ttgttcggtg
     4321 ctgatctcgt cgcgaatctg ccgcgcattc tcgcgcgctg caaaaatgca cgccgtaatc
     4381 gacgcatgcc gcgtcgtatc gaagcacaga gcgtgcacca gcgccgttac gcccacccat
     4441 cgcgtcggct cgcttccgtc gccctcctgc caccccaacc ctggcgcggc ccccaccgcc
     4501 gccagcactc tctgccagcg acgctccgcg ctcacccgag accggtccag catcagcccc
     4561 atgttgatgt cgatcaaccg cgccgtatgc tcagcccgct ccagataccg gctcgtccag
     4621 cacaaactgt ccgctacgcg tgaaagcatc ccaccgcctc aactcacaac tgacaacgct
     4681 gccggacagc ttcgctgttc gtcagctccc cagcacccac gtatccttgc tcccgccgcc
     4741 ctgcgacgag ttcaccacca gcgatccctg ccgcagcgcc acgcgcgtca ggccgcccgg
     4801 cacaatcgtc accttgtcgc cgaacaaaat gtagggccgc aaatccacat gacgcggtcc
     4861 aatcgatcca tcctccagca ggcacggcgc acgagaaaag ctcagcgtcg gttgcgcgat
     4921 gtagttccgc ggatcggcgt cgatccttct ccgaaactcc tcccgttctg ccgacgaact
     4981 ctgcgggcca accagcatcc cgtatccgcc gctctcgccc accgccttca ccaccagctt
     5041 ttccagattc tccttcacat acccgcgctg ttttgcatct gtcagcagat acgtctccac
     5101 gttattcaaa atcggctctt cgcccaggta gtactggatg atcgccggaa cgtacgcata
     5161 aatggccttg tcatcggcaa cgcccgtgcc gaaggcattc gccagcgtca cattccc
//
LOCUS       DASZYN010000427         7041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_469351, whole genome shotgun sequence.
ACCESSION   DASZYN010000427 DASZYN010000000
VERSION     DASZYN010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469351"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VHE33_11020"
     CDS             <1..200
                     /locus_tag="VHE33_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98028.1"
                     /translation="YSVLIAAGLELTAQSHTILSTLCQCTRETLEMTASDPVRVDLVI
                     RRVEVRGSCEPGGSVPPPRMA"
     gene            456..1583
                     /locus_tag="VHE33_11025"
     CDS             456..1583
                     /locus_tag="VHE33_11025"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HVT98029.1"
                     /translation="MERWDAIVVGAGPAGCAAAFDLATAGRSVLLLDKAEFPRHKACA
                     GGLTRKSLRALRYSVAPVVRETVSAIVVERRSGQAATLASRNAVCAMTVRQELDHFCW
                     QQTLAAGAQFERIGSITGFACEREEVTVRTAEQEYRGRFLVGADGVHSRIRQLFYGDR
                     PAWFRRGFALEAKVYPTNGQRRDLVFDFAPVRQGYGWVFPKGDHLNVGLGVYNDAGQE
                     KLDRPRLEMYIRERFGDSRAEHVTGQYLGFGAEAAPAAEERVFLVGDAGGFADALTGE
                     GIHGAIASGQAAAAAIEGELRGAKKAQTAFGALTAQLRQDLQLSSSGSRWFYGNLDVG
                     YRALTAPVFRNAVINAYANGTRIAALANAVRRFAQVFATPA"
     gene            1806..2861
                     /gene="coxB"
                     /locus_tag="VHE33_11030"
     CDS             1806..2861
                     /gene="coxB"
                     /locus_tag="VHE33_11030"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HVT98030.1"
                     /translation="MRFLVRDQGARAIRRIGPLILLLMGWASAARADTPQSSPSIFAP
                     SSTPADTILGLGWFVIAILSGIFVTVGGLLTWAIIRYREREGDDESEPPQIYGSTQVE
                     LAWTVIPILIVVVLFLTTARIIFAIQDAPKPQAALDVTVVGHQFWWEFRYPKLGIVTA
                     NELHVPVSTALDPEPTYLHLLSADVDHSFWVPQLAGKTDLIPNHPNDTWIEPKHTGVY
                     LGQCAQFCGIEHAKMLLRVYVDTPEQFEAWVQQQKQPGAQDAKAAEGRHLFETEACTN
                     CHTIRGTAARGTFGPDLTHVASRATLASGAALNTPENLHAWIESPDSLKQGALMPAMQ
                     LSNEQIDELVAYLETLR"
     gene            3161..4852
                     /gene="ctaD"
                     /locus_tag="VHE33_11035"
     CDS             3161..4852
                     /gene="ctaD"
                     /locus_tag="VHE33_11035"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HVT98031.1"
                     /translation="MADQTLPIAGVDVTPRRRSTALLLEWLHGWVVTVDHKRLGILYM
                     VYGIFFLLVAGVEALAIRIQLAWPGMHFLSPETYNEFFTMHGTTMVFLVGMTIIFGFG
                     NYLVPLMIGARDMAFPRLNAFSFWLTAFGGLLLYYSFIGGFGLYGMGAAPDTMWWAYS
                     PLTEKAFSPGHAVDYWALSLIVMGFGTLGTAINIIATVLSLRCKGMSLMRMPLFVWLM
                     LVVSGLTLVTITPLTAAQIMLTIDRYLGGHFFDTQAGGSAVVWAHFFWIFGHPEVYVL
                     VLPAFAIANEIIPVFSRKAIFGYPAMVAASVGIAFVSLGVWAHHMFTIGMTSVGNTFF
                     VLSTMLVGIPTGIKIFNWLATLWGGKIRFAPPMLFCMGFLFQFLIAGLTGIMLSVAPW
                     DWQLHNSYFVIAHFHYVLVGAIVFNIFAGIYYWYPKVTGRMLSDKLGKWHFWLFLIGF
                     HVTFDTMHFVGFLGMPRSIYTYEPDRGWNGLNFWISVGGMIQGIAVLIFAWNLVHSYF
                     KGRKAGNDPWDAWTLEWAVPSPPPVFNFAADPVVHSRRPLWDLKHPDDPDWKYEA"
     gene            4849..5454
                     /locus_tag="VHE33_11040"
     CDS             4849..5454
                     /locus_tag="VHE33_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HVT98032.1"
                     /translation="MSAIPVSPGPVSAGVDRWELPSRGRVGMLSLIIGESAIFTIFVV
                     AYVFYIGKSLTPPYPAQVLEIPFFNTVCLLSSSITIWLAERAIERAKMGLFAAWWAVT
                     IALGGIFIGGTAIEWHKLIYVDGLTIHTNLFGTTFYSLVGLHATHVVVGLIGLLLILI
                     FTLTGHVREKHSERIQVFALYWHFVDAVWVVVFTVVYIVGR"
     gene            5457..6350
                     /locus_tag="VHE33_11045"
     CDS             5457..6350
                     /locus_tag="VHE33_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98033.1"
                     /translation="MTQTTQHETMHSAGPAHGQTIHLPAPTAWPIVLAFGCVMAVAGL
                     VTSLWVTVFGAVLIVSGCVGWFRQVLPHEAHEDVPVVVQPVTITTTRKVVRRLELGPE
                     HRAQLPVETYPILSGLKGGIAGGIAMIFPALAYGLIAQHSIWYPVNLLGGAGVGPSAH
                     VTTAEIAQFHASWLVVAIVIQAVTCCLVGLLYGAMLPMLPNRPVILGGIVAPLLWTGL
                     LHSAMGIINPQLDARIQWGWFVVSQVTFGVVAGLVVAREGKIRTGRGLPFFARMGIEA
                     PGLMESREDAGPPLSHDEEKH"
     gene            6350..>7041
                     /locus_tag="VHE33_11050"
     CDS             6350..>7041
                     /locus_tag="VHE33_11050"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVT98034.1"
                     /translation="MRRWALVAALPLMVALSGCESAPGAKAAEATAPRPEQVMDFKTL
                     YATNCQACHGENGHGGPAMELGNPEYQALVDDATLRKWISGGMPGTEMPAFAQSAGGM
                     LTDAQINAIIAGMRKEWAKTDAFGGATPPPYAQPAGGDAKRGAQVYQARCASCHEKPE
                     RQQVMSPVYLSLVSDQVLRTVIVAGRPDIGQPDWRLQPVSMNGVHIDVYAPIRPLAGQ
                     DVTDIVTYLGSLR"
BASE COUNT         1237 a         1699 c         2617 g         1488 t
ORIGIN      
        1 tgtacagcgt gctgatcgcc gcggggctgg agctgaccgc gcagtcgcac acgatccttt
       61 cgacgttgtg tcagtgcacg cgggagacgc tggagatgac ggcaagcgat ccggtgcggg
      121 tggatctggt gattcggagg gtggaggttc gcggatcctg tgagccgggg ggatcggtgc
      181 cgccgccgcg gatggcttag agagcgggaa gcccggcggc gggatgctct cgggatgctg
      241 aatggttgcg ctgggcggcc ggggttgata cgccgacaga agcccggcat ttttgccaga
      301 gtcggaacgg tggaaccgct attcagtact ttcgattgag gggcggcagg agattcgatt
      361 gaaaggcagc tgatacaagc gttagcttct taggccggaa ttgttacgtc gaagttattc
      421 cttgagacgt acttcctggg catactattt tccgaatgga acggtgggac gcaatcgttg
      481 ttggggccgg accggcgggg tgcgcggcgg cattcgatct ggcgacggcc gggcgctccg
      541 tgctgctgct ggacaaggcg gagtttccgc gacataaggc ttgtgccggc gggctgacgc
      601 gcaaatcgct gcgggcgctg cggtattcgg tggcgccggt ggtgcgggag acggtgtcgg
      661 cgattgtggt ggagcggcgg agcgggcagg cggcaacgct ggcgagccgc aatgcggttt
      721 gtgcgatgac ggtgcggcag gagctggacc acttctgctg gcagcagacc ctggcggcgg
      781 gggcacagtt cgagcggatt ggatccatca ccggcttcgc ctgcgaacgc gaggaggtaa
      841 cggtgaggac ggcggagcaa gagtatcggg gacgattcct ggtgggggcg gacggggtgc
      901 acagccggat tcggcaattg ttctatggag atcggccggc gtggttccgg cggggatttg
      961 cgctggaggc gaaagtctat ccgacgaacg ggcaaaggag ggatctggtc ttcgactttg
     1021 ctccggtgcg gcagggctac gggtgggtat ttcccaaggg cgatcatttg aatgtggggc
     1081 tgggcgttta caacgatgcg ggccaggaga aactggaccg gccgcgcctg gagatgtaca
     1141 tccgagaacg gtttggggat tcgcgcgcgg agcatgtgac ggggcagtat ctgggtttcg
     1201 gtgcggaggc ggctccggca gcggaggagc gggtgtttct ggtgggagat gcgggtgggt
     1261 ttgcggatgc gctgaccggc gaggggattc acggagcgat cgccagcggg caggcggcgg
     1321 cggcggcgat tgagggagaa ctgcgcgggg caaagaaggc gcagacggcg ttcggcgcgc
     1381 tgacggcgca gttgcggcag gacctgcagc tttcgtcgtc ggggtctcga tggttctacg
     1441 ggaatctcga tgtggggtat cgcgcgctga cggcaccggt atttcgcaac gcggtgatta
     1501 acgcgtatgc gaacggaaca aggatcgcgg cgctggcgaa tgcggtgcga aggttcgcgc
     1561 aggtgttcgc gactcccgcg taaggcgaag gagagaaagt gaagctgaca ttctggtgga
     1621 gcagtccagg cgatctttgc tgataagatt gtctttcagc gagacgcaga ctgcatccga
     1681 cgctcacgcg aaagccgctg caaccacgcg gctgacatca gatccagttc ttcgaatcac
     1741 tccccggttc tgactccaac caactaactg cagcagcatc aaccgcattt ctgacgcgag
     1801 agcagatgag attcctggtc cgggatcaag gcgcaagggc aattcggcgg ataggtccgc
     1861 tgattctcct gctgatgggc tgggcatcgg cggcgagggc agatacgccg caaagttcgc
     1921 cgagcatttt cgcgccttct tcaacacccg ccgatacgat tctggggctg ggctggttcg
     1981 tgatcgcgat cctgagcggg atcttcgtca cggtcggagg actgctgacg tgggcgatca
     2041 tccgttatcg cgagcgtgag ggggatgacg agagcgagcc gccgcagatt tacggcagca
     2101 cgcaggtgga gctggcgtgg acggtgattc cgattctgat tgtggtggta ctgtttctga
     2161 ccacggcgcg gatcattttt gcgattcagg acgcgcccaa gccgcaggcg gcgctggatg
     2221 tgacggtggt ggggcaccag ttctggtggg agtttcgtta tccgaagctg ggcattgtga
     2281 cagcgaatga actgcacgtg ccggtgagca cggcgctcga tcctgagccg acgtatctgc
     2341 atttactgtc ggcggacgtg gatcacagct tttgggtgcc gcagctggca ggcaagacgg
     2401 atctgattcc gaatcatccg aacgatacgt ggatcgagcc aaagcatacg ggcgtgtacc
     2461 tggggcagtg tgcgcagttc tgcgggatcg agcacgccaa gatgctgctg cgggtttatg
     2521 tggatacgcc ggagcaattc gaggcatggg tgcagcagca gaagcagccg ggcgcgcagg
     2581 atgcgaaggc ggcagagggg cggcacctgt ttgaaacaga agcctgcacg aattgccaca
     2641 cgatccgggg cacggcggcg cggggaacgt ttgggccgga tttaacgcat gtggcgagcc
     2701 gcgcgacgct ggcttccgga gcggcgctga atacgccgga gaatttgcat gcgtggatcg
     2761 agagcccgga ttcgttgaaa cagggggctc tgatgccggc gatgcagctg agcaatgagc
     2821 agatcgatga gttggtggcg tatttggaga cgctgcgtta gatgaaaaaa ccggacctca
     2881 tcctgcaggg gctaaagccc ccattttttg gggacgcctt ttcggcacga ctgaacagct
     2941 tgcggaaaaa gccggtgatt caggcgaaag acttccctca gcggctaaag ccggagtcat
     3001 tttgccgcct ttacggcaca gctgacgctg tgccctttca aagaacccga gtttttgggc
     3061 aagctgtgaa gtcgtgccct gatacaaatg ccgtcgagtg tcgaggtttt cggcaatctg
     3121 tgaagagaca acggatcggg gatttctcag gacgatagaa atggcagacc agaccttacc
     3181 gattgcggga gtggatgtaa cgccgcggcg gcgctcgacg gcactgttgc tggagtggct
     3241 gcacgggtgg gtggtgacgg tcgatcacaa gcggctgggg attctgtata tggtgtacgg
     3301 aatcttcttc ctgctggtgg caggagtgga ggcgctggcg attcgcatcc agctggcgtg
     3361 gccgggcatg cattttcttt cgccggagac gtacaacgag ttcttcacca tgcacggcac
     3421 gaccatggtg tttctcgtgg gcatgacgat catcttcggg tttggaaatt acctggtgcc
     3481 gctgatgatc ggcgcgcgcg atatggcgtt tccgcggctg aacgctttca gcttctggct
     3541 gacggcgttt ggcggcttgt tgctgtacta cagctttatt ggcgggtttg ggttgtacgg
     3601 gatgggcgcg gcgccggaca cgatgtggtg ggcgtattcg ccactgacgg agaaggcgtt
     3661 ttcgccagga cacgcggtgg actactgggc tctgtcgctg attgtgatgg gatttgggac
     3721 gctggggacg gccatcaata tcatcgcgac cgtgctgtcg ctgcgctgca agggaatgtc
     3781 gctgatgcgg atgcctctgt tcgtgtggct gatgctggtg gtgagcggac tgacgctggt
     3841 gacgattacg ccactgacag cggcacaaat tatgctgacc atcgatcgct acctgggcgg
     3901 gcacttcttc gacacgcagg cgggcggatc ggcggtggtg tgggcgcact tcttctggat
     3961 cttcgggcat ccagaggtgt acgtgctggt gctgccggca ttcgcgatcg cgaacgagat
     4021 cattccggtg ttttcgcgga aggcgatttt tgggtatccg gcgatggtgg cggcctcggt
     4081 ggggattgcg tttgtgagcc tgggggtgtg ggcgcaccac atgttcacga tcgggatgac
     4141 gtcggtgggg aacacgttct ttgtgttgtc gacgatgctg gtgggaattc cgacgggcat
     4201 caagattttc aactggctgg cgacgctgtg gggcgggaag atccgtttcg ctccgccgat
     4261 gctgttttgt atggggttcc tgttccagtt tttgattgcg gggctcacgg ggatcatgct
     4321 gagcgtggcg ccgtgggact ggcagctgca caattcttat tttgtgattg cgcacttcca
     4381 ttacgtgctg gtgggcgcga tcgtgttcaa catttttgcc ggtatctatt actggtaccc
     4441 caaggtgacg gggcggatgc tgagcgacaa gctcggcaag tggcacttct ggctgttcct
     4501 catcggattt cacgtcacgt tcgacacgat gcactttgtg ggatttctgg gaatgccgcg
     4561 ctcgatttac acctacgagc cggatcgcgg atggaacggg ctcaacttct ggatatcagt
     4621 gggcgggatg attcagggga tcgcggtgct gatcttcgcg tggaacctgg tgcattcgta
     4681 cttcaaaggc aggaaggcgg gaaacgatcc gtgggatgcg tggacgctgg agtgggcggt
     4741 gccgtcgccg ccgccggtat ttaacttcgc cgccgatccg gtggtgcaca gccggcggcc
     4801 gctgtgggat ctgaagcatc ccgacgatcc ggactggaag tacgaagcat gagcgcgatc
     4861 cctgtatctc caggccctgt ctctgcgggc gtcgaccgat gggagctgcc ttcgcgcggg
     4921 cgagtgggca tgctgtcgct gatcattggc gagagtgcga tcttcacgat ttttgtggtg
     4981 gcgtatgtgt tctacatcgg gaagagcctg acgccgccgt atccggcgca ggtgctggag
     5041 attcctttct tcaataccgt gtgtctgctt tcgagcagca tcacgatctg gctggcggag
     5101 cgggcgattg aacgcgcgaa gatggggctg ttcgcggcgt ggtgggcggt gacgattgcg
     5161 ctgggcggga tctttatcgg tggcacagcg atcgagtggc acaagctgat ctacgtggat
     5221 ggactgacga ttcacacgaa tttgtttggg acaacgttct actcgctggt agggctgcac
     5281 gcgacgcatg tggtggtggg gctgatcggg ctgctgctga ttttgatttt tacgctgacg
     5341 gggcatgtgc gagagaagca cagcgaacga atccaggtgt tcgcgctgta ctggcacttt
     5401 gtggatgcgg tgtgggtggt ggttttcacg gtggtgtaca tcgtgggcag gtagagatga
     5461 cgcagacgac acagcacgag acgatgcaca gcgcgggacc ggcgcacgga cagacgattc
     5521 atctgcccgc gccgacggcg tggccgattg tgctggcgtt cggatgcgtg atggcggtgg
     5581 cggggctggt gacgagcctg tgggtaacgg tgttcggcgc ggtgctgatc gtgagcggat
     5641 gcgtggggtg gtttcggcag gtgctgccgc acgaggcaca cgaagatgtg ccggtggtgg
     5701 tgcagcccgt aacgatcaca acgacgcgga aggtggtccg gcggcttgag cttggccccg
     5761 agcatcgggc gcagttgccg gtggagacgt atcccatatt gagcggactc aagggcggga
     5821 ttgcgggcgg gattgcgatg atttttccgg cgctggcata cgggctgatc gcgcagcaca
     5881 gcatctggta tccggtgaac ctgctgggcg gcgcgggagt ggggccgtcg gcgcacgtga
     5941 cgactgcgga gattgcgcag tttcatgcga gctggctggt ggtagctatt gtgatccagg
     6001 cagttacctg ttgtctggtg ggactgctgt acggggcgat gctgcccatg ctgccgaatc
     6061 ggccggtgat tctgggcgga attgtggcgc ctctgttgtg gacggggctg ctgcactcgg
     6121 cgatggggat tatcaatccg cagctggacg cgcgtatcca gtggggatgg tttgtggttt
     6181 cgcaggtgac gtttggcgtg gtggcaggac ttgtggtggc gcgcgagggg aagatccgca
     6241 cagggcgcgg gctgccgttc tttgcgcgca tgggaatcga ggcaccgggg ctgatggagt
     6301 cgcgtgagga tgcgggacct ccgctgagtc acgacgagga gaagcactga tgcggcggtg
     6361 ggcactggtc gcggcgttgc cgctcatggt ggcgctgagc gggtgcgaga gcgcgccggg
     6421 agcgaaggca gcggaggcga ccgcaccgag gccggaacag gtgatggatt tcaagacgct
     6481 gtatgcgacg aattgccagg cgtgccatgg agagaatggg catggcggac cggcgatgga
     6541 attggggaat ccggagtatc aggcgctggt ggacgatgcg acgctgcgga agtggatttc
     6601 cggtgggatg ccggggacgg agatgccggc gtttgcgcag tcagcgggcg ggatgctgac
     6661 ggatgcgcag atcaacgcga tcattgcggg aatgcggaaa gagtgggcga agacggatgc
     6721 ttttggcgga gcaactcctc cgccgtatgc gcagccagcg gggggcgatg cgaagcgtgg
     6781 ggcgcaggtg tatcaggcgc ggtgcgcttc gtgtcatgag aaaccggaac ggcagcaggt
     6841 gatgagcccg gtgtacctgt cgctggtgag cgatcaggta ctgcggacgg tgattgtggc
     6901 aggacggccg gatattgggc agccggactg gcgtttgcag ccagtctcta tgaatggagt
     6961 acatatcgac gtctacgcgc caattcgacc gctggcgggg caggatgtca ccgacattgt
     7021 tacgtatttg ggatcgctgc g
//
LOCUS       DASZYN010000428        13445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_920163, whole genome shotgun sequence.
ACCESSION   DASZYN010000428 DASZYN010000000
VERSION     DASZYN010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 13445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13445
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920163"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="VHE33_11055"
     CDS             <1..1425
                     /locus_tag="VHE33_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT98035.1"
                     /translation="DRDKLCAFIDQHRTDEPSRNLAQYVSLALYLTPPPQLTPSVEEQ
                     DLPPDANGVELILPLVRRLAADIDLHHIWVENHAAYDDLANKLHGPLSQMIVATNYYL
                     KMPASSYSGRHFLVVIEPLFSPLETNARVYGSDYVVVASPHNQQISMDLVRHAYLHYE
                     IEPLLYQRANSMDRLMPILRAVDDAPLEFEFKSDIVALVIECMIRAIEARTMNTGIPD
                     VHVPTNLPHSETEQYERARTVAQQRQEAVRQQVVNHDMTQGYVLAQYFYNQLRTFEKS
                     PESLSEAIGPMVYGMDMSVETHRAQQITFDAQGEGDIMRRSPQRPAQANELDLAEVKL
                     MKGDVAGASRIAQKALDTHSGDAGQADFILARADLMNGKIDDAEAAFRNTIAASKNPR
                     MVAWSHIYIGRIFDVKEQREDAVAEYRAALASRDGQPDTKAAAEDGIRKPFALPPHDQ
                     SSNSGESDQPPAKPPTSSNPAHPQ"
     gene            1460..2260
                     /gene="rnc"
                     /locus_tag="VHE33_11060"
     CDS             1460..2260
                     /gene="rnc"
                     /locus_tag="VHE33_11060"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HVT98036.1"
                     /translation="MKLSAHSSVDAPPPADPAIADSSAIDALETLLNHRFSHPELLRR
                     AITHSSLAHERGALEPQREDNETLEFLGDAVVGMVAAEHVFRLYPELSEGDLTRLRGA
                     LVSRRHLAEVAANLDLGRFLLLGRGEERSGGRSKTALLANAMEAVIGALYLDAGLEAA
                     RALIDAHVVAPVVSVLREQLTARGGMGDFKSVLQELLQARKQGQPDYRITAETGPDHH
                     KRFFVEVRVAGAVLAEGEGRTRKHAEQDAARRAIDKLHEKPSAMEPEA"
     gene            2257..3141
                     /gene="lepB"
                     /locus_tag="VHE33_11065"
     CDS             2257..3141
                     /gene="lepB"
                     /locus_tag="VHE33_11065"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HVT98037.1"
                     /translation="MSVPPQPGPEPHHPPPSAPDFGTDPGQEPVAESFGTLPLSGPPP
                     PPPARRKIVLFPGMMEMIRSLLVVLVIALFILTFIVQPFRIPSESMEHTLLVGDFLLV
                     NKMAFAPPGRWRWLLPYRDVQRGDIIVFHFPPDPPEHVVKRVIGLPGDHIHLQNALAW
                     VNGQPFDEPWVSFEPAYPDDFRDNFPTELYTDPGVDAHWWMQMRTDVREGDLVVPPNS
                     YFVLGDNRNFSRDSRYWGFVPRDHIVGRPFVVYFSLREPSTTDVAPPADDRLGQKNDL
                     WYKIANFARWNRLLRVVR"
     gene            3158..3940
                     /gene="lepB"
                     /locus_tag="VHE33_11070"
     CDS             3158..3940
                     /gene="lepB"
                     /locus_tag="VHE33_11070"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HVT98038.1"
                     /translation="MAEVEEQHTSKHKHEETPLEAFASICGVLAVGLFVLTFVFQNFA
                     IPSSSMVRALLVGDHVLVDRITLAPRARWAPFVNYRDVHRGDIVVFYKPTLEANGDHL
                     FLVKRVIGIPGDHIHLRSGIVYRNGVAVDEPYAGKPTDADYDAYRDDFPSVSPSLSGN
                     VTAEWAEALPGFIQGQDLVVPQGQYFCMGDNRIVSLDSRYWGFVPRPNIVGRPLFVYW
                     SFETPDSQEYKTSLADRVGWILHETIHFFDQTRWRRTLHRVK"
     gene            3943..6513
                     /locus_tag="VHE33_11075"
     CDS             3943..6513
                     /locus_tag="VHE33_11075"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HVT98039.1"
                     /translation="MPSLEELVSSEESRSPRPFPWLRVAIIGAFIALLLVAIFLPPYI
                     NLGKYRRRITASMSEALGRPVYVGGMQLRMLPTPGIVMTDFTVNEDPSFGYEPALHAT
                     TVVASLRLTSLWRGRLEVSRISLDEANLNIVRNPGGEWSIGSVLVRASQISNAPTAQR
                     TASARPRFPYIEATNARIDFKDGAEKKPFSLMNAEFSMWQANADEWRIRLRAQPVRTD
                     LQLHLSDTGELNVEGSLHRAPALDDMPVDLSAEWSGAQLGQVTRLLAGMDSGWRGDLD
                     LTTNIRGNIGDLQLQSRLRVSDLRRQEFQPPATVDIDATCRSQYQHLGQRLNGITCFW
                     PVSDGHLLLTGSIHGLQKPNADLQLEINSIPASFPIRFFQLIRPGAQNVTVGGIVNGS
                     FHLVTADHPLLTGTADVTGVSLRYAGGTLNLPTMHFVAPSAQPEPSHHARHTQPASAP
                     AKNSIQLLPLAVPMGEPQPLIADSRFTRDGFELHLTGPASLARLLPAIANLGVFQNAA
                     NLVAPRGRATLNLTATAEWMRPLAERAGNGIATSGSIVLESTQLRPAFLPAPVEVQSA
                     DIGLDPSQISWQNVALTYQKMPMHGSLEFPVLCTDPAGCPATFTLAMGNTSANAIETA
                     LGVNPDSGFLGKLLSFSENHPAWPTLQGQIQCDQLDLGLLPLHNVTASLSVAGNALSF
                     SSLDAAALGGTLHWTGDMRIEDGTPRWNLKARLTGASAAEAAALFDEKWSPGQLNADA
                     TLSLNGYRGSDLAGSAAGDFSFTWQNGAFPSGAPAKTTTISTAAPVPFERWTARGTIA
                     SNRLKIVSGGVSRSGRTSALRGSITFSRDLDLTLETRRGDEKITGTLAQPVVSAASQP
                     "
     gene            complement(6491..8353)
                     /locus_tag="VHE33_11080"
     CDS             complement(6491..8353)
                     /locus_tag="VHE33_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98040.1"
                     /translation="MRFFVCSAAVAGACLLFAQSGFSQDPSTQVESSQPESPRGQVLF
                     SGQPAQPGNTAAVDAGGAAAVSDAERRSVAVTAWNLEVHLSPREQAMEVHARVTLRNA
                     GAAPLNRVALQLSSTLHFETVGFRGKKLVFRQHEIASDADHTGKLNEAVIPLEDALPP
                     DSSTTLDVDYGGTVPLTAERLTAIGAPDATAQASDWDRITEDFTGVRGFGNVVWYPVS
                     SVPVSLKNGSELFNEIGRQKQMDEGAIVSLRLTDEFVSPVPNAVVLNGHLMSGVQPAA
                     MPTASFPGVLTASLPATRLGFETPSFFVARRTAVEGHGLRVLATDADAASAQRYVAAA
                     EPAEPVVTTWLGKAKTSFTILDLPEADDAPAEMADVLATPLSGDDAQHLTPVAIHALA
                     HGAFQSPRVWLNEGVAAFLGSLWIEQSVGQQAALEGLNAGRPALALAEPASPGEGSGE
                     DLLHATSAVYYRTKADYVLWMLRDVVGDQALQSALQQYDPAQDTTPEYFEKLLEKASS
                     RDLKWFFDDWVYRDRGLPDLSIGGVYPSREGLHQVLVAVEIRNDGYAAAEVPVTLKGL
                     GGTVTRRVLVPAHGRITHRMTFSEDPVEVDVNDGSVPEVRDSVHARMITAAKPH"
     gene            complement(8350..9129)
                     /gene="rlmB"
                     /locus_tag="VHE33_11085"
     CDS             complement(8350..9129)
                     /gene="rlmB"
                     /locus_tag="VHE33_11085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HVT98041.1"
                     /translation="MDVLFGIHPVEEALRSGTRRFDHLCVARERHDQRLAEVVALARA
                     AGVPVRAEPREALTRLAGTTGHQGVVAVVGERAFLGVEDLLDGPSPRLVLALDGIEDP
                     QNLGALLRTAEAAGVDGVLFTERRSAPLSAVAVKASAGAAEHLRLARVVNLVRAIETL
                     KEQNLWVVGLDERGTTDYDRYDFRSNTVIVLGREGAGLHELVRKTCDHLLRIPMAGAV
                     SSLNVSAAGAVVMFEAARQRRTLASAEGSSRASKPQKGLGS"
     gene            9243..9953
                     /locus_tag="VHE33_11090"
     CDS             9243..9953
                     /locus_tag="VHE33_11090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVT98042.1"
                     /translation="MDLTQKVEKLVAEGVFRGSSPVCEIDMSTATLQLAEVVRSENAR
                     VVEGLRRHDPALLDELILQYQHRLLRYLLYLTGNREMAEDLFQETWMRVLMRGAQFNG
                     NSRFDTWLFTIARNLVIDLRRRRSMASLEEMCEGGDEDRPFEVPSQEKSPFDHLATLE
                     NGQMVAEALLTLDPLHREVLVLRFHEEMSLEEIARMTRAPLSTVKSRLYRGLASLKPH
                     VRSAARPAAPASDRMEVR"
     gene            9950..10288
                     /locus_tag="VHE33_11095"
     CDS             9950..10288
                     /locus_tag="VHE33_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98043.1"
                     /translation="MNSEVLRPGRRVTGHPIPEVVLRTRRSVMEAAIELQAQRTRKRR
                     HVGIALLAMGVLVLLVAPLLWSTANELSTGEHFFELPVLVLTLFLVFLAAFFAILLVT
                     WRDRAARAER"
     gene            10307..10858
                     /locus_tag="VHE33_11100"
     CDS             10307..10858
                     /locus_tag="VHE33_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HVT98044.1"
                     /translation="MNPDLRQRAELHPEGFSGEWSLIPWWSVVGGIVVFGLMQYLMWV
                     IVPAHRTHPPNLPFWFRFYIDISIGLLAGLYVLMIGYITRDSPRRGMSVPLWMVVCVV
                     IPSGIGAVLYFLLRLPVLSVCPACGARIESDYHFCPQCAYQVAPCCGNCHRNARITDL
                     YCVYCGHHLAEDHPPARLHAFAE"
     gene            11010..11360
                     /locus_tag="VHE33_11105"
     CDS             11010..11360
                     /locus_tag="VHE33_11105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVT98045.1"
                     /translation="MEKQSRANLLQGTLDMLVLRTLLYGPAHGHQIGKHIQRTTNEFL
                     QMQHGSLYPALHRLEKKGWVSAKWETAPDRNREFKYYRLTEKGRKQLVAEESQWKQMT
                     EAVARVMWPAAEES"
     gene            11362..>13445
                     /locus_tag="VHE33_11110"
     CDS             11362..>13445
                     /locus_tag="VHE33_11110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HVT98046.1"
                     /translation="MNRWPSRKRDADLERELRSDLELEEEEQRERGASPEEAHYAALR
                     AFGNPTVIREQTRTEWSWSWTESLLRDLRYGMRTLRRTPGFTVIAILIMAIGIGANIA
                     LFTVVRGVLLKPLPYNDPGRLVLMYEADRSQPHPAPWLPVDAGSFWEWQRSAGSLAEL
                     AMVSPFQGYIVSAEGGKLPEVVGAAWCTNNFFPLLGVQPMLGRLFTPDDDRAGAPATA
                     VITASFWKSRYASDPNIVGKPIWLDAKIHTIIGVLPATFVYSGAFGGAADQIWTPVQH
                     DAPASLLKAYGDHEFLVVGRLASGVSMTALEARLDALQKQIKSAHPEPAVHRDVIART
                     MLDDAVHNYKTPLYALLGATGCVLLIACMNVASLLVARAASRSREFAIRTALGGGRLR
                     LIRERLIESLLLSMVGGAVGLLLAGGALKWFIHVRDDMNRVESIHFDAVVAAFTVAVV
                     ALCALFSGLISAVSSGRNNVLTGLQESSRSHSGGRSRAILRRVLLTVEVGLTIVLLVG
                     AGLLLKSFERLRSSDLGVPVDNVLTLRVGLPDVRYPKPEQRIAFFEQLIQRVRALPGV
                     SSAGLVSTAPGEGWNGDDLMSIAEHPPLPPGQGFDFMLRGADPGYFSAIGLPILKGRT
                     FSEDERMDRANVVLISRGAAQEYFPGEDPIGKHLRAIDGSGTWQVIGVVGDVRWDVAQ
                     PPKPTLYWPIYG"
BASE COUNT         2387 a         4623 c         3893 g         2542 t
ORIGIN      
        1 gatcgcgaca agctctgcgc cttcatcgat cagcatcgca ccgacgagcc ttcgcgcaat
       61 ctcgcacaat atgtgtccct cgcgctctac ctcacgccgc ctccgcaact gacgccgagc
      121 gtcgaagagc aggacctgcc gccggatgcc aatggcgtgg agctgattct gcctctcgtt
      181 cgccgccttg ccgccgacat cgacctgcac cacatctggg ttgagaacca cgccgcttac
      241 gacgatctcg cgaataagct gcacggcccg ctgtcgcaga tgatcgtcgc cacaaactat
      301 tacctcaaga tgcccgcttc gtcctacagc ggccgccact ttctcgtcgt gatcgagccg
      361 ctcttttcgc cgctggaaac caatgcgcgc gtctacggct ccgactatgt tgtggtggcg
      421 tcaccccaca atcagcagat ctccatggac ctggtgcgcc acgcgtatct gcattacgag
      481 atcgagccgc tcctttatca gcgcgccaac tccatggacc ggctcatgcc catcctgcgc
      541 gccgttgacg acgctcctct ggaattcgaa ttcaagagcg atatcgtcgc cctggtcatc
      601 gagtgcatga ttcgagccat cgaagcgcgc accatgaata ccggcatccc cgatgtccac
      661 gtccccacca acctgcccca cagcgagacc gagcaatacg aacgggcacg caccgtcgct
      721 cagcagcgcc aggaggcggt ccggcagcag gtggtcaatc acgacatgac ccagggctat
      781 gtattggcgc agtacttcta caaccagctg cgaacttttg agaaaagccc cgagagcctc
      841 agcgaagcca tcggccccat ggtctacggc atggacatga gcgtcgagac gcatcgcgcc
      901 cagcagatca ccttcgatgc acagggtgag ggcgacatca tgcgccgctc tccgcaacgt
      961 ccggcccagg cgaacgaact cgatctcgcc gaggtgaaac tgatgaaggg ggatgtggcc
     1021 ggcgcctccc ggatcgcaca gaaggcgctc gacacgcaca gtggcgatgc aggccaggcc
     1081 gatttcatcc ttgcccgcgc ggacctgatg aacggcaaga tcgacgacgc cgaagctgcg
     1141 ttccgcaaca ccatcgccgc gagcaaaaat ccccgcatgg tggcctggtc gcacatctac
     1201 atcggccgca tcttcgatgt gaaggaacag cgtgaggacg cggtggccga atatcgcgct
     1261 gcgctcgcca gccgcgacgg ccagccggac accaaagccg cagcggagga tggcatcagg
     1321 aaacctttcg cgctgccgcc gcacgaccag tcttcgaatt ccggtgagag cgatcagccg
     1381 cccgcgaaac ctccgacatc atcgaacccg gcccatcctc agtaatgcgc tctgcaataa
     1441 tcatggcagc ggcccgcgca tgaagctctc cgcccattca tccgtcgacg cgcctccccc
     1501 ggccgatcca gccatcgccg attcctccgc catcgatgca cttgagactc tcctgaatca
     1561 ccgtttttct catcctgaac tgctgcgtcg cgccatcacg cacagctcgc tcgcccacga
     1621 gcgaggagcc ctggaaccgc agcgcgagga taacgagacg ctcgagtttc tcggcgatgc
     1681 ggtggtcggc atggtcgccg ccgagcacgt tttccgtctt tatcccgagc tgtccgaggg
     1741 cgacctcacc cgtctgcgcg gagcgctggt cagccggcgg catctggctg aagtggcagc
     1801 gaatcttgat ctcggccgct tccttttgct cggccgcggc gaagaacgca gcggcggccg
     1861 cagcaaaact gcgcttctcg ccaatgccat ggaggcggtg atcggcgcgc tctatctgga
     1921 cgccggcctt gaagccgcgc gcgcgctgat cgacgcacac gtcgtggctc cggtcgtcag
     1981 cgtgctgcgc gagcagctta ccgcgcgcgg aggcatgggc gacttcaagt ccgtgctgca
     2041 ggaactgctg caggcgcgca aacagggtca gcccgattac cgcatcaccg ccgaaaccgg
     2101 ccccgatcat cacaaacgct tcttcgtcga ggtgcgcgtc gccggcgccg tgcttgccga
     2161 aggagagggc cgcacccgca agcatgccga gcaggatgca gcccggcgcg cgatcgacaa
     2221 actccacgag aaaccgagtg cgatggagcc tgaggcatga gcgttcctcc gcaacccggt
     2281 cctgagccgc accaccctcc gccgtctgcg ccggatttcg gcaccgatcc gggccaggag
     2341 ccggttgcgg agtccttcgg cacattgccg ctgtcggggc ccccgccgcc acccccggct
     2401 cgccgaaaga tcgtcctgtt tccaggcatg atggagatga tccgttcgct cctggtggtt
     2461 ctggtgatcg cgctcttcat cctcaccttc atcgtgcagc ccttccgcat cccttcggaa
     2521 tcgatggaac acacgctgct ggtgggtgat ttcctcctcg tgaataagat ggcattcgcg
     2581 cctcccggcc gctggagatg gctcctcccc tatcgcgatg tccagcgcgg cgacatcatc
     2641 gtcttccact ttcctcccga tccgcccgaa cacgtcgtca agcgcgtcat cggtctgccc
     2701 ggcgatcata tccatctcca gaacgccctg gcctgggtca atggccaacc cttcgacgag
     2761 ccgtgggtct cctttgagcc cgcttatcct gacgacttcc gcgacaattt ccccaccgag
     2821 ctctacaccg atcccggcgt cgatgcgcac tggtggatgc agatgcgcac cgatgtccgc
     2881 gagggcgacc tcgtcgtgcc gccgaacagc tacttcgtgc tgggcgacaa tcgcaatttc
     2941 agccgcgaca gccgctactg gggctttgtt ccgcgcgacc acatcgtagg ccgccccttc
     3001 gttgtctatt tctctctgcg cgagccgtcc accaccgatg tcgcaccacc tgcggatgat
     3061 agactcggac agaaaaacga tctttggtac aagatcgcta acttcgcgcg ctggaatcgc
     3121 ctgctgcgcg tggttcgcta aacctacctg gagtctgatg gcagaagttg aggagcagca
     3181 cacgtcgaag cacaagcacg aggaaacacc cctcgaggct ttcgcctcca tttgcggggt
     3241 tctcgccgtc gggctctttg tcctcacctt tgtctttcag aacttcgcga tcccctcctc
     3301 ctccatggtc cgggcgttgc tggtcggcga ccatgttctt gtcgatcgca tcacgctcgc
     3361 gccccgcgcc cgctgggctc ctttcgtcaa ctatcgcgac gtgcaccgcg gcgacatcgt
     3421 cgttttctac aagcccacgc tcgaggcgaa cggcgatcat ctcttcctcg tcaagcgcgt
     3481 catcggcatc ccgggcgacc acatccacct tcgcagcggc attgtttacc gcaatggtgt
     3541 cgccgtcgat gagccctacg cgggcaaacc caccgacgcg gactacgatg cctaccgcga
     3601 cgactttccc tcggtctcgc ccagcctgag cggcaatgtc acggccgagt gggctgaggc
     3661 gctgcccggc ttcatccagg gtcaggacct ggtcgttccc cagggccagt acttctgtat
     3721 gggcgataac cgcatcgtca gcctcgacag ccgctactgg ggcttcgttc cccggccgaa
     3781 catcgtcggc cgaccgctct tcgtctattg gtccttcgag acgccggatt cgcaggagta
     3841 caaaacctca ctggccgatc gggtcggatg gattctgcat gaaacgatcc atttcttcga
     3901 tcaaacccgc tggcgccgca ccctccatcg agtgaaatag ccatgccttc gctggaggag
     3961 ttggtgtcct ccgaggagtc tcgcagccct cggccctttc cgtggctgcg tgtcgccatc
     4021 atcggcgcct tcatcgccct gctgctggtg gctattttcc ttccgccgta catcaatctt
     4081 ggaaaatatc gccgccgcat caccgccagc atgtcggaag cgctcggccg ccccgtctac
     4141 gtgggaggca tgcagctgcg catgctgccg acgccgggaa tcgtcatgac cgacttcacc
     4201 gtcaatgagg atccctcgtt cggttacgag ccggctcttc atgccacaac ggtggtcgct
     4261 tcccttcgcc tcacttcgct ttggcgcgga cgtcttgagg tcagccgcat cagcctcgat
     4321 gaagccaatc tcaacatcgt ccgtaacccc gggggcgaat ggagtatcgg ttcggttttg
     4381 gtgcgcgcct cgcagatttc caatgccccc accgcgcaac ggacggccag cgcacgtccg
     4441 cgattcccct acatcgaagc caccaacgcc cgcattgact ttaaggatgg tgcggagaag
     4501 aagcccttct ccctgatgaa cgccgagttc tccatgtggc aggccaatgc cgacgagtgg
     4561 cgcattcgcc tgcgcgcgca gcctgtccgc accgacctcc agcttcacct ctccgacacg
     4621 ggcgagctga acgtcgaagg ctctctccac cgtgctcccg cactcgatga catgcccgtg
     4681 gacttgagcg cagagtggag cggcgcccag ctgggccagg tcacccgtct tctggccggt
     4741 atggacagcg gatggcgcgg cgacctcgac ctgaccacca acattcgcgg caacatcggc
     4801 gatctgcagc tgcagtcgcg cctccgtgtt tccgaccttc gccgccagga attccagccg
     4861 cccgcgacgg tcgacatcga cgccacctgc cgcagccagt accaacacct cgggcaaaga
     4921 ctaaacggca tcacctgctt ctggcctgtt tctgacgggc atcttcttct caccggcagc
     4981 attcacggcc tgcagaaacc caacgccgat ctccagctcg agatcaacag tattccggcc
     5041 tctttcccca tccgcttctt tcagctgatt cgtccgggag cgcagaacgt caccgtcggc
     5101 gggatcgtca acggcagttt tcatctggtt accgccgacc acccgctgct gaccgggacc
     5161 gcggatgtta ccggcgtctc gctgcgctat gccggaggca cgctgaatct tcccaccatg
     5221 cactttgtcg ctccgtccgc gcagcctgag ccgtcgcacc acgcacggca cacgcagccg
     5281 gcttcggccc cagcaaaaaa ctcgattcag ctcctgcctc tggccgttcc catgggagaa
     5341 ccgcagcctc tcatcgcgga ttcgcgtttc actcgtgatg gatttgaact ccatctcacc
     5401 ggaccggcct cgctggcgcg gcttctgccg gcgatcgcca atctgggcgt gttccaaaac
     5461 gccgccaacc tcgtcgcgcc gcgcggacgc gccacgctga acctcaccgc taccgccgaa
     5521 tggatgcggc ctctcgctga aagagccggc aatggcatcg ccaccagcgg ttcgatcgtt
     5581 ctggaatcca cgcagctgag gccggcgttt ttgcccgcgc cggtggaggt gcagtctgct
     5641 gatattggcc tcgatccctc gcagatctcg tggcaaaacg ttgcccttac ctaccagaag
     5701 atgcccatgc acggctcgct ggaatttccc gtgctgtgta cggatccggc cggctgtccc
     5761 gcgacattca cactcgcgat gggcaatacc agcgcgaatg ccatcgaaac cgcccttggg
     5821 gtcaatccgg acagcgggtt tctcggcaag ctgctcagct tcagcgaaaa ccatccggcc
     5881 tggccaacgc tgcagggcca gatccagtgc gatcagctcg acctcggcct gctcccgctg
     5941 cacaatgtca ccgcatcgct cagcgttgcc ggcaatgcgc tcagtttctc ctcgctcgac
     6001 gccgccgcgc tgggaggaac gctccactgg acgggcgaca tgcgcatcga agacggcaca
     6061 ccgcggtgga acctgaaggc gcgcctcacc ggcgccagcg ctgccgaagc tgccgctctg
     6121 tttgatgaga agtggagccc gggtcagcta aacgccgacg ccacgctcag cctgaatggc
     6181 taccgtggct ccgatctggc cggctccgct gccggcgact tctcctttac gtggcaaaat
     6241 ggcgcgttcc ccagcggcgc gccagccaaa accacgacca tcagcaccgc cgcgcccgtt
     6301 cccttcgagc gctggaccgc aaggggaacg atcgcctcca acaggttgaa gatcgttagc
     6361 ggcggcgttt cccggtccgg cagaaccagc gcgctccgcg gttccatcac ctttagtcgc
     6421 gaccttgatc tcaccctcga aacgcgccgc ggcgatgaga aaatcaccgg cactcttgcc
     6481 caacccgttg ttagtgcggc ttcgcagccg taatcattcg cgcgtgaacg ctgtcgcgga
     6541 cctcgggcac gctgccgtca ttcacatcga cctcgaccgg atcctcagag aacgtcatgc
     6601 gatgcgtgat gcgcccgtgc gccggaacca gcacgcgccg cgtcaccgtt ccgcccaggc
     6661 ccttcagcgt cacgggtact tcggccgcgg catagccgtc attgcggatc tccaccgcca
     6721 ctaatacctg atgcaggcct tcgcggctcg gatagacccc gccaatcgag agatccggca
     6781 gcccgcggtc gcgatacacc cagtcgtcga agaaccattt cagatcccgg ctgcttgcct
     6841 tttcaaggag cttctcaaaa tactccggcg tcgtatcctg tgccggatcg tactgctgca
     6901 gcgcactctg caaagcctgg tcgcccacca catcgcgcag catccacagc acgtagtccg
     6961 ccttggtccg gtagtaaacc gcgctggtcg cgtgcagcag atcttcaccc gatccttctc
     7021 ctggcgacgc cggctccgcc agtgccagtg cgggacggcc ggcattcagt ccttccagag
     7081 cggcttgttg tcccacactt tgttcgatcc ataacgaccc taagaaggcc gctacgccct
     7141 cgttcagcca cacccgcggc gactggaatg ccccatgcgc gagcgcgtgg atcgcaaccg
     7201 gcgtcagatg ctgcgcgtcg tcgccggaaa gcggcgtcgc cagcacatcg gccatctctg
     7261 ccggggcatc gtccgcctcc ggcaagtcga gaatcgtaaa gctcgtcttc gccttgccga
     7321 gccaggttgt aaccaccggc tcggccggtt ccgccgctgc gacatatcgc tgtgcgctgg
     7381 cagcatcggc atcggtcgcc aacacgcgca gtccgtggcc ctccacggcg gttcgccggg
     7441 ccacaaagaa cgaaggcgtt tcgaacccga ggcgcgttgc gggaagcgac gccgtcagca
     7501 cccccggaaa cgaggccgtg ggcatggctg ccggctgcac gccggacatg aggtgtccgt
     7561 tcagcacgac agcgttggga accggcgaca caaattcgtc ggtcaatcgc aggctcacta
     7621 tcgcgccctc atccatctgc ttctggcgtc cgatctcgtt gaacagctcg gaaccatttt
     7681 tcagcgacac gggcacggag ctcaccggat accacaccac attgccgaag ccgcgcacgc
     7741 cggtgaagtc ctccgtgatg cggtcccagt ccgatgcctg cgccgtcgca tcgggcgcac
     7801 cgatcgccgt cagccgctcg gcggtcagcg gaactgttcc gccgtaatcc acatcgagcg
     7861 ttgtcgagga atccggaggc agcgcgtctt caagaggaat cacggcctca ttgagcttgc
     7921 cggtgtggtc cgcatcgctc gcgatctcgt gctgacggaa caccagcttc tttccgcgga
     7981 agcccaccgt ctcaaagtgc agtgtcgagg aaagctgcag cgcgactctg ttgagcggcg
     8041 ctgcgccggc attccgcagc gtcacgcgtg cgtgcacttc catggcctgc tcgcgcgggc
     8101 tcaggtgcac ttcgagattc cacgccgtga ccgccaccga gcggcgctct gcatcggaga
     8161 cggccgccgc cccgcccgca tcgacggctg cggtgttgcc cggctgcgcc ggctgtcctg
     8221 agaacagcac ctgcccacga ggtgactcgg gctgtgatga ctcaacctgc gtgctcgggt
     8281 cctgactgaa tccagactgc gcgaacagca gacaggcgcc ggccactgcg gcgctgcaaa
     8341 cgaaaaacct catgaaccca gacctttttg cggtttcgac gcacggctcg agccttccgc
     8401 cgatgccaag gtccgccgct ggcgtgccgc ctcaaacatc accacagctc ccgccgccga
     8461 cacgttgagc gagctcactg cgcccgccat cggaattcgc agcagatgat cgcatgtctt
     8521 ccgcaccagc tcgtgcaatc cggcgccttc gcgtcccagc acaatcaccg tgttggaccg
     8581 gaaatcgtag cggtcgtaat ccgtcgtgcc gcgctcgtcc agccccacga cccacaggtt
     8641 ctgctccttc agcgtctcaa tcgcgcgcac cagattgacc acgcgggcca ggcgcagatg
     8701 ctccgccgct ccggccgacg ccttcaccgc caccgcgctc agcggcgccg accggcgctc
     8761 ggtgaacagc actccatcga ctccggcggc ctcggcggtt cggagcagcg cgcccaggtt
     8821 ctgcggatcc tcgatgccat cgagggccag caccagccgc gggctgggac cgtccagcag
     8881 gtcttccacg ccgagaaatg cccgctcccc cacgacagcc acgaccccct gatgccccgt
     8941 ggtgccggcg aggcgggtca gtgcctccct gggctcggct cgcactggaa ctccggcggc
     9001 ccgggccagg gcaacaacct ctgccagccg ctggtcgtgc cgctcccgcg ccacgcagag
     9061 atggtcgaaa cggcgcgttc cggatcgcag cgcttcttcc accgggtgaa tcccgaacag
     9121 gacatccatt ccatcaagtc tatcgtttca ggagcgctca aatcccactg ataatggtag
     9181 agttatccta tcgtccggca gtcggcggcc ccgcccccgc gaggcacgca gaaagggcag
     9241 ccatggatct gacgcaaaag gtcgagaaac tcgtcgccga aggcgtcttt cgtggttctt
     9301 ctcccgtctg tgaaattgac atgagtacgg caacgctcca gctcgcggaa gtcgtccggt
     9361 cagaaaatgc ccgcgtcgtc gaaggcctgc gccgtcacga tcccgcactc ctcgatgaac
     9421 tgatcctgca ataccagcat cgcctgctgc gctacctgct ctacctcacc ggcaatcgcg
     9481 agatggccga agacctcttc caggaaacct ggatgcgggt gctgatgcgc ggcgctcagt
     9541 tcaacggcaa ctcgcgtttc gatacctggc tgttcaccat cgcccgcaac ctcgtcatcg
     9601 atctccgccg ccgccgttcc atggcgagcc tcgaagagat gtgcgaaggc ggcgatgagg
     9661 atcgcccctt cgaagttccc agccaggaaa agtctccttt cgaccatctg gccacgctcg
     9721 agaacgggca gatggtcgct gaagcgctgc tcacgctcga cccgctccat cgcgaagtcc
     9781 tggtgctgcg cttccatgag gagatgtcgc tggaagagat tgcccgcatg acgcgcgcgc
     9841 cgctttccac ggtgaagtcg cgtctctatc gcgggcttgc atcgctcaag ccgcatgtgc
     9901 gatcggcggc tcgcccggcc gcgccggctt ccgatcggat ggaggttcga tgaacagcga
     9961 agtcctccgt ccaggcagaa gagtgaccgg ccacccaatc cccgaagtcg tgctccgtac
    10021 acgccgttcc gtgatggaag ctgcaatcga acttcaggca cagcggacgc gcaagcgccg
    10081 gcatgtgggc atcgcgctgc tggcgatggg tgtactcgtg ctcctcgttg caccgctcct
    10141 ctggagcacc gcgaacgaac tcagtacagg agagcacttt ttcgagctgc ctgtgctggt
    10201 cctgacgctg tttctggtct ttctcgctgc cttcttcgcg attctgctgg tgacgtggag
    10261 agaccgggcc gcgcgcgcag agcgatagcc cttaacaagc tgactcatga accctgatct
    10321 tcgccaacgc gcagagttgc accctgaagg tttctccggc gagtggagcc tcattccctg
    10381 gtggtccgtg gtcggaggca tcgtcgtctt cggcctgatg cagtacctca tgtgggtcat
    10441 cgttcccgcc caccgcaccc atccgccgaa cctgcccttc tggttccgtt tctatatcga
    10501 catcagcatc ggcctgctgg ccgggctcta cgtgctgatg atcgggtaca tcacgcgcga
    10561 ctccccgcgg cgcggcatga gtgtgcccct gtggatggtc gtttgcgtcg tgatcccgag
    10621 cggcatcggc gcggtactct acttcctgct gcgtctgccg gtgctgtcgg tgtgcccggc
    10681 ctgtggtgcg cgtatcgaga gcgactacca cttctgcccg cagtgtgcct atcaggtcgc
    10741 gccgtgctgc gggaactgcc atcgcaacgc ccggatcacc gatctctact gcgtgtactg
    10801 cggccatcac ctggccgagg accatccgcc ggcacgactg cacgccttcg ccgaataggt
    10861 tgtcggcgag ccatctcgct ccgttctctt tcgaaaaaaa cagaaccggg gggcgggtgt
    10921 ggtccattgg tcccatttcc ctcagaagcc cttgacactc gatagtcgga tgtctaccat
    10981 cgactccaac tcagggttgg atgtctacca tggaaaagca atcgcgagcc aacctgctgc
    11041 agggcaccct cgacatgctc gtcctgcgca cgctgctcta cggtcccgcg cacgggcacc
    11101 agatcggcaa gcacattcag cggaccacca acgagttcct gcagatgcag catggctcgc
    11161 tctatccggc tttgcacagg ctggaaaaga agggctgggt cagcgcgaag tgggaaacgg
    11221 cgccggatcg caaccgcgaa ttcaagtatt accggctcac ggaaaaaggc cgaaagcagc
    11281 tggtcgccga agagtcgcag tggaagcaga tgaccgaggc cgtggctcgg gtgatgtggc
    11341 cggcggccga ggagagctga gatgaaccgc tggccgagcc gcaagcgcga tgccgacctg
    11401 gaacgcgaac tgcgctccga cctcgaactg gaagaggaag aacagcggga gcgcggcgct
    11461 tcgccggagg aagcgcacta tgcggcattg cgcgctttcg gcaacccgac cgtgatccgc
    11521 gaacagacgc gtaccgaatg gtcctggagc tggaccgaat ccctgctgcg cgatctgcgc
    11581 tatgggatgc gtaccctgcg ccgcactccg ggcttcacgg tcatcgccat tttgatcatg
    11641 gccatcggca tcggagccaa cattgccttg ttcaccgtcg tgcgcggcgt gctgctcaag
    11701 ccgctgcctt acaacgatcc aggccgtttg gtgctgatgt acgaagcgga caggagccag
    11761 ccgcatccgg cgccatggct tcccgtggat gcgggaagtt tctgggagtg gcagcgctcg
    11821 gccggatcgc tggcggagct ggcaatggtc tctcccttcc agggatacat cgtctcggcg
    11881 gaaggcggaa agctgcccga agtcgtggga gcggcgtggt gcacgaataa tttctttccg
    11941 ctgctgggcg tacaacccat gctcggccgc ttgttcacgc ctgacgatga tcgagccggc
    12001 gcgccggcca ccgctgtgat caccgcgagt ttctggaaga gccgttacgc gtccgacccg
    12061 aacattgtgg gcaagcccat ctggctcgat gcaaaaatcc atacgatcat cggcgttctc
    12121 cctgccacgt ttgtctactc gggagccttt gggggcgccg ccgaccagat ctggactcca
    12181 gtacaacatg atgctcccgc ttccctgctg aaggcttacg gcgatcacga gtttctcgtt
    12241 gttggccgtc tcgcttccgg agtttcgatg accgcactcg aggcgcggct cgacgcactg
    12301 cagaagcaga tcaagtccgc ccatcccgag cccgcggtcc accgcgacgt cattgccaga
    12361 accatgctgg atgacgcggt gcataactac aagacaccgc tgtatgcgct gctcggcgct
    12421 accggatgcg tgctgctcat cgcgtgcatg aacgtcgcca gtcttctcgt cgcacgcgcc
    12481 gcatcgcgca gccgcgagtt tgcgattcgc accgccctcg gcggcggacg cctgcgcctg
    12541 atccgtgaac ggctgatcga gagcctgttg ctctccatgg tgggcggcgc tgtcggcctg
    12601 ctcctggccg ggggtgcgct caaatggttc attcatgtgc gtgatgacat gaatcgagtg
    12661 gaatcgatcc atttcgacgc cgtggttgcg gcgttcactg tggccgtcgt cgctctctgc
    12721 gctctttttt ccggcctgat ctcggctgtg agcagcgggc gcaacaatgt cctgacagga
    12781 ttacaggaaa gctcccgctc ccacagcgga gggcgctctc gcgcaatcct gcgacgcgtt
    12841 ttgctgacgg tggaagtcgg actgacgatc gtcctgctgg tcggcgccgg gctgctcctg
    12901 aagagtttcg agcggctccg atcctctgac cttggcgtgc cggtggacaa cgtgctcacc
    12961 ttgcgcgtgg gcttgcctga cgtccgctat ccgaagcctg agcagaggat cgctttcttt
    13021 gaacaactga tccagcgagt gcgcgctcta cccggcgtct catccgcagg cctggtgagc
    13081 accgctccgg gcgaaggctg gaatggcgac gatctgatga gcatcgctga gcatccccct
    13141 ctgcccccgg gtcagggctt cgacttcatg ctgcgaggtg cggatcccgg ctacttctcg
    13201 gcgatcggac ttcccatcct gaaagggcgc acgttcagcg aagacgagcg catggaccgg
    13261 gccaacgttg tgctgatcag ccgcggcgcg gcgcaggagt atttcccggg cgaagacccg
    13321 atcggtaagc atctccgggc gatcgacggc agcggcacct ggcaggtgat cggtgtggtt
    13381 ggcgacgtgc gatgggatgt cgcgcaaccg cccaagccga cactctactg gccgatttat
    13441 ggcaa
//
LOCUS       DASZYN010000429         4668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1113584, whole genome shotgun sequence.
ACCESSION   DASZYN010000429 DASZYN010000000
VERSION     DASZYN010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113584"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..883
                     /locus_tag="VHE33_11115"
     CDS             152..883
                     /locus_tag="VHE33_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT98047.1"
                     /translation="MTIESSAMTAREPSTTPELGTILVIEDDPRMQKVLRRMFMEENY
                     SVLVAGDGQTGLELFRAHRPVAVLLDLILPQISGRELCQSIKAISVETPVIVLSAVTE
                     VVDKVLLLELGADDYVTKPFSPRELMARVQASIRRRKRPVQATVYRFGDCELDFEKMV
                     ARRAGVQVVMTSHEFKLMKFFTENAERVLTRDVLLNEVWGYNSYPTTRTVDNQILKLR
                     QKLEPDPANPKHLLTIYGAGYKFVP"
     gene            923..2713
                     /locus_tag="VHE33_11120"
     CDS             923..2713
                     /locus_tag="VHE33_11120"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVT98048.1"
                     /translation="MRKNRGVRTRALLIAAMAAVIAAATLASLLLIRHRLLQQVTQGL
                     SGNLIRSVNTFQDLQAERLKALDRENALMADLPTLKALMTTNDTRTIENEAADFRNVS
                     GSDLFALADMEEHVVAAFTNRSTTDAQAAADLQAVLAKPGTLYLVSGGRLFACSVRPL
                     YFGSEAKGALLGYVISGFAVDRELLEQISRATMVDATFISDGQILVSTLPDGTQESLS
                     RQLLPAAGSAARSMEVGEEKYLTVTTDLTSRASAPLHLVVLRSFSAADRSIRQIDQLV
                     IAAGLLAMVLGTLLMLALSYSVTRPLEELAAGVRAFGSGDSAHLLPYRGTREVRDLSA
                     SFSKMRQEIQQTNRALLESERLATIGRMASSVSHDLRHYLAAVYANAEFLASERLSEA
                     ERAEILSDIHAAVYGTTDLLESMLIFSRTGSAIRRSPEVMATLMERAIGLVEKHPDAG
                     NVTFTPSYGDAAATLAVVDVKQIERALYNLLLNACQSRRTPGDAVHVTARLEADSDQI
                     HFEVADDGGGVPEDIRTSLFQPFVSQGKQKGSGLGLTLAHCIAAEHGGYLVLVSSRPG
                     ETVFRMTIARALLGEGIYQEKSEAEVAHEA"
     gene            2703..4208
                     /locus_tag="VHE33_11125"
     CDS             2703..4208
                     /locus_tag="VHE33_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98049.1"
                     /translation="MKHSYRGKLDKAHVLKRSVPGLAVLLVVWVSPLRAQAPNENELA
                     KKVQLLTDAVDKAQAQVEESKRQLADLRQQLADVRRQIGGGGDDPDPPDDAAKLAAQV
                     EALKENQAMLQSEVATHDQSKVESASKYPVKLSGLILLNGFVNTSQVDVPAAPGVALY
                     GSGSTGATMRQTVLGIDARGPHIFGAASRADLRTDFFGSTAGTGSYGTAGFLRLRTAH
                     AALDWASTEVFFSLDRPLINPEQPTSLTSVANPALAWSGNLWSWNPQLGVRHDQSIGE
                     DTRFRVETALIDVGDPPYAAVPYTVSTNEQSRWPGVQSRLAVVRGREDRGFQLGAGGY
                     FSKRRTVYGGSNFDSWAAALDYRQPLPGGVELSGNFYRGLALGGLGGGAYKDYGVRPD
                     PDRPGAFYIRPFDDVGGWAQLKERAGERLEFNEAVGVDDVPAHEVRRYSANASGSYLN
                     LSRTQTVMGNVIFSPSSWLQFSLDYRHLTSFWASYPATSAHVIGVAAGYKF"
     gene            4208..>4668
                     /locus_tag="VHE33_11130"
     CDS             4208..>4668
                     /locus_tag="VHE33_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98050.1"
                     /translation="MRRVLCRVLPCSAALLTAVLSFGQDLRGRIAYPGNVTAMPPAVV
                     WLKPLQPVDPAPAEPHKSYTLLQKNRSFIPHLLVVPVGSHVEFPNEDPFYHNVFSLFD
                     GKRFDLGLYEAGSTKTVTFSREGVSYIFCNIHPEMSAVVLALATPFYAIAD"
BASE COUNT          906 a         1178 c         1635 g          949 t
ORIGIN      
        1 gaagaaggaa atacgggagg atcaatggca gtcggtggga gcaccatgag acggctgcca
       61 tgctgttgat gtcagaatgt caatacattg tcatttgtga ccttagaaat gtcatttagc
      121 cgttggggcg ggcacaatga ggaatatgga aatgacgatt gaatcttctg ccatgacagc
      181 gcgggaacct tcgactacgc cggaactggg aacgatcctg gtgatcgagg acgacccgcg
      241 gatgcagaag gttttgcgtc gcatgttcat ggaggaaaat tattcggtgc tggttgccgg
      301 cgacgggcaa acggggctgg agttgttccg cgcgcatcgt ccggttgctg ttctgctcga
      361 tctgattctt ccgcagattt cggggcgcga gctttgccag agtattaagg ccatcagcgt
      421 cgagacgccg gtgattgtgc taagcgctgt tacggaagta gtggataagg ttctgctgct
      481 cgagttgggc gccgacgatt atgtgacgaa gcctttcagc ccgcgggagc tgatggcgcg
      541 ggtacaggcg tcgatccggc ggcgcaagcg ccccgtgcag gcgacagtgt accggttcgg
      601 cgactgcgag ctggacttcg agaagatggt agcgcggcgg gccggagtgc aggtggtgat
      661 gacgtcgcac gagttcaagc tgatgaagtt cttcacggag aatgccgagc gcgtgctgac
      721 acgcgacgtg ctgctgaacg aggtgtgggg gtacaactct tatccgacga cgcgcacggt
      781 cgataatcag attctgaagc tgcggcagaa gctggagcct gacccggcga atccgaagca
      841 cttgctgacc atctacggag caggctacaa atttgtccca tagaggggcg gcagaatggg
      901 gccgggttcg ggaacagcag gcatgcgcaa aaatcgaggt gtaagaacgc gtgcgctgct
      961 gatcgctgcg atggcggcag tgattgctgc agcgacgctg gccagcctgc tgctgatccg
     1021 gcatcggctg ctgcagcagg tgacccaggg gctttcgggc aacctgatcc gttccgtgaa
     1081 cactttccag gatctgcagg ccgagcgttt gaaggcgctg gatcgcgaaa atgcgctgat
     1141 ggcggatttg ccgacgctaa aggcgctgat gacgaccaac gacacgcgca ccatcgaaaa
     1201 tgaggcggcg gattttcgca acgtcagcgg cagcgatttg tttgcccttg cggatatgga
     1261 agagcacgtc gtcgccgcat tcaccaatcg ctcgacaacg gatgcgcagg cggcggccga
     1321 tctgcaggct gttctggcaa agcccggcac cctctatctg gtaagtggag gaaggctgtt
     1381 tgcgtgttcg gtgcggccac tgtatttcgg cagcgaggca aagggggcgc tgctggggta
     1441 tgtgatcagc ggttttgctg tcgatcgcga acttctggaa caaatcagcc gcgcgaccat
     1501 ggtggatgcg acgttcatca gtgacggaca gattctggtg agcactcttc ccgacggcac
     1561 gcaggaatcg ttgagccgcc agcttttgcc ggcggcgggc tcggctgcac ggtcgatgga
     1621 ggttggcgag gaaaaatacc tcacggtgac gaccgatttg acgtcgcgtg cctctgcgcc
     1681 acttcacctg gtggtgctgc gatcgttcag cgcggcggat cgttcgatcc ggcagatcga
     1741 tcagttggtg attgcggcag gactgctggc gatggttttg ggcacgctgc tgatgctcgc
     1801 gctttcctac tccgtgacac gaccgctgga agaactggcg gcgggtgtac gcgctttcgg
     1861 gtcaggcgac agcgcgcatc tgctgccgta tcggggtaca cgggaggtgc gggatctgag
     1921 cgcgtccttt tcgaagatgc gccaggagat tcagcagacg aatcgggctc tgctggagtc
     1981 agaacggctg gcgaccattg ggcggatggc aagctcggtc tcgcacgatc tgcggcacta
     2041 tctggcggcg gtgtatgcga acgcggaatt cctggcgtcg gagcgattgt cggaggcaga
     2101 gcgggcagag attctgtccg atattcacgc cgccgtctat gggacgacgg atttgctgga
     2161 gtcgatgctg atcttcagcc ggacggggtc ggccattcgg cgctcaccgg aggtgatggc
     2221 gacactgatg gaacgggcga ttgggctggt ggagaagcat ccggatgcgg gaaacgtgac
     2281 gttcacgccg agctacgggg atgcggcggc cacgctggcg gtggtggacg tgaaacagat
     2341 cgagcgcgcc ctgtacaatt tgttgctcaa cgcatgccag tcgcggcgga cgcccggaga
     2401 cgccgtacat gtgacggcca gactggaagc tgacagcgac cagattcatt ttgaagtggc
     2461 cgatgacgga gggggtgttc cggaagacat tcggacgagc ctgttccagc cgtttgtcag
     2521 ccagggaaaa cagaagggat cgggactggg cctgacgctg gcgcattgca tcgcggcgga
     2581 gcatggcggg tatctggtgc tggtgagcag ccgcccgggc gagacggtct ttcggatgac
     2641 gattgcgcga gccttgctcg gagagggcat ctaccaggag aaatcggaag ctgaggtggc
     2701 gcatgaagca tagctaccgt ggaaagttgg acaaggcgca cgttctgaag cgcagcgtgc
     2761 caggtcttgc ggtcttgctg gtggtttggg tgtcgccgct tcgagcgcag gcgccgaatg
     2821 agaacgaatt ggcgaagaag gtacagctcc tgaccgatgc ggtggataag gcgcaggcgc
     2881 aggtggaaga gtcaaaacgg cagttggccg atcttcggca gcaactcgcg gatgtgcggc
     2941 ggcaaatcgg cggtggtggc gacgatccag atccaccgga cgatgcggcg aagctggccg
     3001 cccaggttga ggcgctcaag gaaaaccagg cgatgctgca atcggaagtc gcgacccacg
     3061 atcaatcgaa ggtggagagc gcctcgaagt atccggtgaa gttgtcggga ttgattctgc
     3121 tgaatggatt cgtgaacacg agccaggtgg atgttccggc agctccgggt gtcgcgttgt
     3181 atggttcggg gagcacgggg gcgacgatgc ggcagacggt gctggggatc gacgcgcgtg
     3241 ggccacatat ttttggggcg gcgagccggg cggatcttcg cacagatttt tttggaagca
     3301 cggcgggaac aggtagctac ggaaccgcag gcttcttgcg actgcgcaca gcgcatgcgg
     3361 ctttggactg ggccagcacg gaggtgttct tttcgctcga tcgcccgctg attaacccgg
     3421 aacagcccac atcgctgacg tcggtggcaa atccagcgct ggcctggtcg ggaaaccttt
     3481 ggtcatggaa tccccagctt ggggtgcgcc acgatcagtc gattggagag gacacacgat
     3541 tcagggtgga gacagcattg atcgatgtgg gcgatccgcc gtacgctgcc gtgccataca
     3601 cggtttcgac gaatgaacag agccggtggc cgggcgtgca gtcgcggctt gccgtggtgc
     3661 ggggccggga ggatcgcggg ttccagctgg gtgcaggtgg atatttttct aagcgccgga
     3721 cggtctatgg aggatcgaac ttcgattcgt gggccgcggc gctggattat cggcagccgc
     3781 tgccgggtgg tgtggaactg agcgggaatt tctatcgtgg gctcgcattg ggcggcctgg
     3841 gcggaggcgc gtacaaggat tacggtgtaa gacccgaccc ggataggccg ggagcttttt
     3901 acattcggcc cttcgacgat gtgggcggat gggcacaact gaaagagcgg gccggagagc
     3961 ggctggaatt caacgaagcg gtgggcgtgg acgatgtgcc cgcccatgag gtgaggcgct
     4021 actctgcaaa tgcatcaggg agctatctga atttgtcgcg aacgcagacc gttatgggca
     4081 atgtgatctt tagtccgagc tcgtggctgc agttttccct ggactaccgg catctgacca
     4141 gtttttgggc gagctaccca gcgaccagtg ctcatgtaat cggcgttgcg gccgggtata
     4201 agttctgatg aggcgagttc tctgcagagt tctgccgtgc agcgcggcgc ttttgacggc
     4261 ggtgctgagt ttcggccagg acctgcgtgg gcggatcgca tatccgggta atgttacggc
     4321 gatgcccccc gctgtggtgt ggctgaaacc gttgcagccg gtggatccgg cgccggcgga
     4381 accacacaag tcgtacacgc tgctgcaaaa gaaccggagc ttcattccgc atctgctcgt
     4441 ggttccggtg ggcagtcacg tcgagtttcc caacgaagac ccgttctacc acaatgtttt
     4501 ttcgctcttc gatgggaagc ggttcgacct gggcctgtat gaagcggggt ccacgaagac
     4561 ggtgacgttt tcacgcgaag gcgtttccta tatcttctgc aatattcatc cggagatgag
     4621 cgcggtggtg ctggcgctgg cgacgccttt ctatgcgatc gcagacaa
//
LOCUS       DASZYN010000430         3195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_469544, whole genome shotgun sequence.
ACCESSION   DASZYN010000430 DASZYN010000000
VERSION     DASZYN010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469544"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VHE33_11135"
     CDS             complement(<1..398)
                     /locus_tag="VHE33_11135"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT98051.1"
                     /translation="MGAVLEPPVATTADAITPPRPPSSADAAAVPAANPWIVAISVML
                     ATFMEVLDTSIASVALPYIAGTLAASNSEATWVLTSYLIANAVVLPASNWFSLRFGRK
                     RFLITCVCLFTVASFWCGAAPSLGMLLLARV"
     gene            658..1680
                     /locus_tag="VHE33_11140"
     CDS             658..1680
                     /locus_tag="VHE33_11140"
                     /inference="COORDINATES: protein motif:HMM:NF025388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HVT98052.1"
                     /translation="MTLTRDNHFVPQLYLKNFASASGEVYEYRILVSHSRVPLWKPVH
                     VAGTGYERNLYTRLVSGEDADDIEQWLNREFETPANEAIQRVLQDRELSRNDWERLAR
                     FLASQIVRTPAFLIKNLPRWNQMAPKVLEQTMRDVEERLREAKRLGQQVIIDNPSSDT
                     EYVPIRVERKDLPEEKRVQLTTKMVVGRGLWFYSMKHLLTKTLKVLPRHNWEILEAPV
                     GLPWFTSDDPAICLNFRSDTDYDFDGGWNRERGNILFPLSPRHLMVTEIGGTFFGRRV
                     PSRYHARLFRRIIAQHSHRRIYSSAIEEKIPQLKPRTVDAKALQTERALWVSYYEDQS
                     SAERAL"
     gene            complement(1704..2591)
                     /locus_tag="VHE33_11145"
     CDS             complement(1704..2591)
                     /locus_tag="VHE33_11145"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HVT98053.1"
                     /translation="MQPRGHIDPLQDFLRIRPRLFGIAYRMLGSAAEAEDVVQDVWLR
                     WQASDRSAILDPPAFLTTATTRLAINVAQSARARRETYIGPWLPEPVDTGSDPLLGAV
                     RGEALQFAVLLLLEKLSPAERAAYILREAFDYPYREIAGILQLEEANTRQLVTRARQH
                     IAEGRRAPVRSAEQRQLLEAFIGAARSGDLAALESLFASDVVSSSDGGGQVRAARVPI
                     AGRERVARFIAAFASHFWTGVTLTWIEANGQPSVLIERENMPIAVATIDASEEGIHQI
                     LWMMQPSKLASIQGQKNQR"
     gene            2755..>3195
                     /locus_tag="VHE33_11150"
     CDS             2755..>3195
                     /locus_tag="VHE33_11150"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HVT98054.1"
                     /translation="MKIVVIGGTGLIGSKLVQQLRDQGQEVVAASPSTGINSITGEGL
                     AEALKDASSVVDVTNSPSWEDAAVLQFFETSTRNLLDAEGRAGVRHHIALSVVGTDRM
                     LASGFFRAKMAQEKLIQASSIPYTIVRATQFFEFVKAIADFATQD"
BASE COUNT          696 a          846 c         1029 g          624 t
ORIGIN      
        1 acgcgggcga ggagcaacat gccgagggag ggcgcggctc cgcaccagaa ggaggcgacg
       61 gtgaaaaggc agacgcaggt aatgaggaag cgcttgcgtc cgaagcggag cgagaaccag
      121 ttgcttgccg gcagcacgac ggcgttggcg attaggtagc tggtgaggac ccaggtggct
      181 tcggaattgg aggccgccag cgtgccggct atataaggaa gggcgaccga ggcgatggag
      241 gtatcgagga cctccatgaa ggtggccagc atgacggaga tggccacgat ccaggggttg
      301 gcggcgggga ctgcggcggc gtcggcggag gaaggcgggc gcgggggggt gatggcatcc
      361 gcggtggtgg ctacgggtgg ttcgagtacc gcccccatgg gggtccttcc tgtgactttt
      421 cagggtaggg ggcggagggg tggcggggct gtggtgtggg tcacaggggt gggggcgaga
      481 taagagtctc ttcggacagg ggcaaagaag gttgcggagt ctaccgagga gccactgacg
      541 atgcgccagc tgtcaaaatc gtattttgcc taagggcaat cgccacgcca acatgcattt
      601 atcatcgggc taatcgagca ggttatagcg tctcgccgta tacgactcgc tttcgccatg
      661 accctgacac gcgacaacca cttcgtgccg cagctatatc tcaagaattt cgcttctgcc
      721 tcgggcgagg tttacgagta ccgcattctt gtatctcatt caagggtgcc cctttggaaa
      781 ccagtacacg tggctggaac gggatacgag cgaaatctat acacgaggct tgtcagtgga
      841 gaagatgcgg acgatattga gcagtggctg aatcgggaat tcgagacacc cgccaatgaa
      901 gcgattcagc gagtgttaca ggatcgggag ctgtcgcgaa atgactggga aaggctcgcg
      961 cgttttctcg catcccaaat cgttagaacg cccgcttttt taataaagaa tcttccacgt
     1021 tggaatcaaa tggctccaaa agttttggag caaactatga gagacgtcga agagcgcctg
     1081 cgtgaagcaa aaagattggg acagcaggtc atcatagaca atcctagcag cgacaccgaa
     1141 tacgtcccca tacgggtaga gcgcaaggat ctccccgagg agaagagggt ccaacttacc
     1201 accaagatgg ttgtcgggcg gggactttgg ttctactcaa tgaagcacct gctgacgaaa
     1261 acgctgaagg ttctgccccg gcacaactgg gagattcttg aggcgccggt aggtttgcca
     1321 tggttcacaa gtgatgatcc agccatttgc ctgaattttc gatctgatac ggactatgac
     1381 tttgacggtg gatggaatcg ggagcgcggc aacatccttt ttccccttag cccacgccat
     1441 ctgatggtta ccgaaattgg cggaactttc tttggaaggc gagttccctc tcgctaccat
     1501 gctcggcttt ttcggaggat aatcgctcaa cattcccaca gaagaatcta ctcttcagcc
     1561 atagaagaga agataccgca gcttaagccc agaactgtgg acgccaaagc actccaaacc
     1621 gaacgagcgc tttgggttag ctactacgaa gatcaatcca gtgcggagcg ggcgctctag
     1681 attagagacg gatctcgttc acgctaccgc tggtttttct ggccctggat cgacgccagc
     1741 ttgctcggtt gcatcatcca cagaatttga tggatgcctt cctctgaggc gtcgatggtg
     1801 gcgacggcaa tgggcatgtt ctcgcgttcg atcaggaccg agggctgtcc gttggcttcg
     1861 atccaggtga gcgtgacacc agtccagaaa tgggaggcga acgcggcaat gaaccgggcc
     1921 acacgctcac ggccggcaat ggggacccgc gctgcacgta cctgtccgcc gccatcggaa
     1981 gaagaaacca cgtccgaggc aaagagactt tcgagggcag ccagatcgcc gctgcgagct
     2041 gcaccgatga aagcttcgag aagctgtctt tgctcggcag agcggacggg ggcgcgacgc
     2101 ccttcggcga tatgttggcg agcgcgggtg accaactggc gggtgttcgc ttcttcgagt
     2161 tgaaggatgc cggcaatctc gcggtaggga tagtcgaacg cttcacggag aatataggct
     2221 gctcgttccg cgggagagag tttttcgagg agcagaagca cggcaaactg cagggcttcg
     2281 ccacgaacgg ctcccagcag gggatcgctg ccggtatcga ccggctcagg gagccatgga
     2341 ccgatgtagg tttcgcggcg agcgcgcgcc gattgagcga cgttgatggc cagtcgcgtg
     2401 gttgccgttg tcaggaatgc gggcggatcg aggatcgcgc tgcgatcgct ggcttgccag
     2461 cgcaaccaga catcctgcac gacgtcctcc gcttcggccg cgctgcccag catgcggtac
     2521 gcgatgccga agaggcgcgg gcggatgcgc agaaaatcct gcagcgggtc gatgtgccca
     2581 cggggctgca ccgttcaatt ctacgaacgt tatgcacaga ttttctgcac acgagcggat
     2641 tttcacagat gaatggcggc gcgatgtcac aaaccgcgag gctgtccggt cctcgtccat
     2701 aagcggcagt tgcggaattg ccaggcttca gccttcaacc acgaaaggac catcatgaaa
     2761 atcgtagtca tcggcggcac cgggctcatc ggatcgaagc tcgtgcaaca gcttcgcgat
     2821 cagggtcagg aggtcgtcgc ggcctcgccc tccaccggca tcaactccat cacaggcgag
     2881 ggattggcgg aagcactgaa ggacgcttcg tcagtcgtgg atgtcaccaa ttccccttcc
     2941 tgggaggatg cagcggtgct gcagttcttc gagacgtcaa cgcgcaacct gctcgacgct
     3001 gaaggccgcg cgggtgtgcg gcatcacatc gcgctgtcgg ttgtggggac ggaccgcatg
     3061 ctggcgagcg gattcttccg cgccaagatg gcgcaggaga agctgatcca ggcctcgtcc
     3121 attccgtaca cgatcgtccg agcgacgcag ttcttcgaat ttgtgaaggc gatcgcggat
     3181 tttgccacgc aggac
//
LOCUS       DASZYN010000431         5306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_920486, whole genome shotgun sequence.
ACCESSION   DASZYN010000431 DASZYN010000000
VERSION     DASZYN010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920486"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VHE33_11155"
     CDS             complement(<1..392)
                     /locus_tag="VHE33_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HVT98055.1"
                     /translation="MPTASDNRTLMHVDVHEVLREGVERTDQALERLLPPLTQVPVSI
                     HGAMRHSVFAGGKRLRPVLAMEAARAMHAVHGGGVQLPAGIEDLGAAIEMLHTYSLIH
                     DDLPALDNDDLRRGKPTCHVAFGEAIAIL"
     gene            complement(376..2133)
                     /locus_tag="VHE33_11160"
     CDS             complement(376..2133)
                     /locus_tag="VHE33_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98056.1"
                     /translation="MRFWFRFPATPSSDATRAQRRRSLLLATVLIAVLPLAWRGASCG
                     QDFDFHLQSWLEVVNHWHHGILYPHWAASANYQAGEPRFVFYPPLSWMLGGVLGLVLP
                     WSWTPVAYVLVALLGAGFAFRQMAREWMPLDSATIAACLYVVNPYMMFVVYERGALAE
                     LLAAIWIPLIVLFALRDRPAVVPLGVTVAALWLSDAPAAVLGCYALVPLVGVAALRER
                     SWRLMARSAAAVPLGLALAGFWLVPAIYQERWVQIHRAIGPLMRVEDSFLFGYVSPVP
                     GPVAEGFNAVYHNGVLQMASWIAVALIVVGAVAAWLSRRRANALWAPLVGLAGLIVFL
                     QFRWSDPLWRYLPELRYLQFPWRWLLVFGLVATGLAGLAMRREQTTRRAIAVRGAVVL
                     LVACGMALLSSLLFWQPCDEEDNIGAQIATFHATGFQGTDEYTPDDAANSRIQQGLPA
                     IRLLNSATAEESPVGDNPEWCPDSTKEIPGAVAITGSGPEQMSATVATGVPGFAVLRL
                     MDYPAWRITVNGSALGEHSRRGDGLLVIPIAAGTTRIDVRWHLTADVWIGLVLTLLAV
                     AITLFSWGKERRRPHANSF"
     gene            complement(2216..4774)
                     /locus_tag="VHE33_11165"
     CDS             complement(2216..4774)
                     /locus_tag="VHE33_11165"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HVT98057.1"
                     /translation="MPLGRSVDIHEEKRRHPFLRVVLILFFLFLLVLAGAVFTAGIWL
                     KHKMQSSLPEIDGALLTPGLTTQVIVRRDHHGVPHIEAANLDDLLFAQGYVTAQDRLW
                     QMDMARRMASGEAAEILGSRLLEHDRMQRMLAVRVTAERLTAALPADERRLFDDYARG
                     VNAYIGSHMDRLPAEFRLLFYQPKPWQPVDSMLVVMSMVQMLDEHWPEKLERERITAR
                     LGPTLAADLYPTGSWRDHPPVTDEVPITMPNQNLPDVPLDESQDGFGAARLDSPILHD
                     DLRRLEQLTGHPPCVECGLDTNPGSNQWVVSGARTASGSPILSNDMHLAQQIPNIWYM
                     TGLKAPGLHAAGVTVPGLPLIVAGHNDHIAWGFTALYGDTQDLYVEHTNGQDQYQTPD
                     GVWHPFEHAQETIHVRGGQDVTVNVERTGHGVVITPLIPGEKRTITLKWNEYDARPGG
                     YPLLAIDEATDWAGFRAALRNWSAPTQNVVYADDQGHIGYQAVGYIPSRPNGLAGVPI
                     DDEQHEWQGYIGFDQLPSAYDPPGGLLATANARVTPNPNPPTTPVAGAPVTGTGPGES
                     STAAQNPAPRPELTLEWGDPYRNERIWKWLSPKDHLTQQQMLTLQTDVYSELDQEIAQ
                     RLAYGIDHSSSVDARLRQAADLMRSWDGAVTIDSAPAAIVEAARSQLFRMLLEPKVGD
                     DWRYYHWAESDFASEQILTNQPAAWLPAEYKTWDDFLTDLVRRGLAAQHAPSDLRTWR
                     YGYAYPVVVEHPLYRLLPWFQRWTGTGVLPQSGDGTTVKQVHRTFGPSQRFTIDWSNI
                     DGATENITMGESGDPLSPYYRDQWPYWYNGKTFAMPFSDAAVAAATAHTLRLQP"
     gene            complement(4902..>5306)
                     /locus_tag="VHE33_11170"
     CDS             complement(4902..>5306)
                     /locus_tag="VHE33_11170"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HVT98058.1"
                     /translation="PASMGDFKGVRTFAQQVKHLAESNYEFFNGWNVPGATAVDPKTI
                     EALKTKDEIVQALKDSYKYAHAAVGTLTPENAGDPVKSFLPPQFKMTKASVAAFCIAH
                     SMDHYGQMVEYLRMNSIIPPMSRPQQQTGNGQ"
BASE COUNT         1045 a         1850 c         1514 g          897 t
ORIGIN      
        1 agaatcgcaa tcgcctcccc aaacgccaca tggcacgtcg gcttgccgcg ccgcagatcg
       61 tcattgtcga gcgcaggcag gtcgtcgtgg atcagcgaat acgtgtgcag catctcgatc
      121 gccgcgccca gatcctcgat acccgccgga agctgcactc cgcctccgtg tactgcatgc
      181 atcgcccgcg ccgcctccat cgccagcacc ggacgcaaac gcttcccgcc ggcaaaaacg
      241 ctgtgccgca tcgcgccatg gatcgacacc gggacctgcg tcagcggcgg cagcagccgc
      301 tccagcgcct gatcggtgcg ctcgactccc tcgcgcaaaa cctcgtgaac atcgacgtgc
      361 atcagggtcc tgttatcaga agctgttggc atggggtcgc cgcctttctt tcccccaact
      421 gaacagtgta atggccaccg cgagaagcgt gagcaccagg ccgatccaca catccgctgt
      481 aaggtgccac cggacatcga tgcgcgtggt tccagcggcg atcggaatca caagaaggcc
      541 atcgccgcgc cgcgaatgct cgcccagtgc ggagccattg acggtaatgc gccacgccgg
      601 atagtccatc agccgaagaa ctgcgaaccc cggaacgccc gtcgcgaccg tggcgctcat
      661 ctgctccgga cccgatccgg tgatagcaac cgctcccgga atctccttcg tcgagtcggg
      721 gcaccattcc ggattgtccc ccaccggcga ttcctcggct gtagcggagt tgagcagccg
      781 gatcgccggc aggccctgct ggattctgct gttggcggcg tcgtccggcg tgtattcatc
      841 cgtaccctgg aatccggtcg catgaaaagt cgcaatctgc gcgccgatgt tgtcttcttc
      901 atcgcacggc tgccagaaca gcaaggagct gagaagcgcc attccacacg ccaccaacaa
      961 aaccacggct ccccgcaccg cgatcgcccg ccgcgtcgtc tgttcccgcc gcatcgccag
     1021 acccgcgagc cccgtagcca ccagcccgaa caccagcagc catcgccacg gaaactgcag
     1081 ataccgcaac tccggaagat accgccacaa cggatcgctc caacggaact gcagaaagac
     1141 aatgaggccc gcgagaccca ccagaggagc ccacagcgca tttgccctcc gccgcgagag
     1201 ccacgcggca acagcgccga ccacgatcag cgccaccgca atccacgaag ccatctgcag
     1261 cacgccgttg tgatagacgg cgttgaaccc ctccgcaacc gggcccggca ctggcgaaac
     1321 atacccaaac agaaagctgt cctccacccg catcagaggt ccaatggcgc gatgaatctg
     1381 cacccaccgc tcctgataga tcgccggcac cagccagaat cctgctagtg ccagacccag
     1441 cggaaccgcg gccgccgagc gcgccatcag ccgccaggag cgctctcgca gcgccgcaac
     1501 ccccaccagc ggaaccagcg catagcatcc aagaacggct gccggtgcgt cgctgagcca
     1561 aagcgccgcc accgtcacac caagcggcac caccgccggc cgatcccgca acgcaaacag
     1621 cacgatcagc ggaatccaga tcgctgccag caactccgcc agtgcgccgc gctcatacac
     1681 cacgaacatc atgtagggat tcaccacata caaacacgcc gcgatggtcg cgctgtcgag
     1741 cggcatccat tccctggcca tctgccgaaa cgcgaacccc gcacccagca gcgcgaccag
     1801 cacataagcc accggcgtcc acgaccacgg caacaccagc cccagcactc cgcccagcat
     1861 ccagctcagc ggcggataaa acacgaagcg aggctccccc gcctgataat tcgccgacgc
     1921 cgcccaatgc gggtacagaa tcccgtgatg ccagtggttg acgacctcaa gccagctctg
     1981 caggtggaaa tcgaagtcct gcccgcacga agccccacgc cacgccagcg gcaacaccgc
     2041 gatcagaacg gtcgccagca gcagcgaccg ccgccgctgc gcgcgcgtcg catcgctgga
     2101 aggagtagcc gggaaacgga accagaatcg cacgtcgtga gtccggggtc attgtacttt
     2161 ccagctgccc gaggccagga atggagtttt cgctactccc aactcacaac tcccctcagg
     2221 gctgcagccg cagcgtgtgc gcggtcgctg ccgccacggc cgcatcactg aagggcatag
     2281 caaaggtctt gccgttgtac cagtatggcc actgatcccg gtaatacggg ctcaacggat
     2341 cgcccgactc gcccatcgtg atgttctccg tcgcgccatc gatattcgac cagtcaatcg
     2401 tgaagcgctg cgacggtcca aatgtgcgat gaacctgctt gaccgtcgtt ccgtcgccgg
     2461 actgcggcag aactcctgtc ccggtccatc tctggaacca tggcaacaat ctgtagagcg
     2521 gatgctccac aacgaccgga taagcgtagc cgtagcgcca ggtgcgcaaa tccgacggcg
     2581 cgtgctgcgc cgcaagaccc ctgcgcacaa gatcggtcaa aaaatcgtcc cacgtcttgt
     2641 attccgcagg cagccacgca gccggctgat tggtcaggat ctgctcactc gcaaaatcag
     2701 actcagccca gtggtaatag cgccagtcgt cgcccacttt gggctcaagc agcatacgaa
     2761 acaactgcga cctcgcagcc tcaacaatcg ccgcaggcgc agagtcgatt gttacggcgc
     2821 cgtcccatga acgcatcagg tccgcagcct gacgcagccg cgcatccaca ctgctggagt
     2881 ggtcaatccc gtaggcgagc cgctgcgcga tctcctgatc gagttcggag tagacgtccg
     2941 tctgcagcgt cagcatctgt tgctgcgtca ggtgatcctt cggtgagagc cacttccaga
     3001 tgcgctcgtt ccggtaggga tcgccccact ccagcgtcaa ctccggacgt ggtgcagggt
     3061 tttgcgccgc agtgctcgat tccccaggcc ctgtccccgt caccggcgcg cccgccaccg
     3121 gcgtcgtcgg tggattcggg ttcggcgtca cgcgcgcgtt cgccgttgcc agcagaccgc
     3181 ccggcggatc atacgcagac ggcagctgat cgaagccgat atacccctgc cactcgtgct
     3241 gctcatcgtc gatcggcacc cccgcaagcc cattcggccg cgatggaata tagccaaccg
     3301 cctgatagcc gatatgcccc tgatcgtccg cgtaaaccac gttctgggtc ggagcgctcc
     3361 agttcctcag cgctgcgcga aaacccgccc agtccgtcgc ctcgtcgata gccagcagcg
     3421 ggtagccacc cggccgcgca tcgtactcgt tccacttgag cgtgatcgtc cgcttctccc
     3481 cagggatcag cggcgtgatc accacaccat gtccggtgcg ctccacattc accgtgacat
     3541 cctggccgcc gcgaacgtgg atcgtctcct gcgcatgctc gaagggatgc cacacgccat
     3601 cgggcgtctg gtactggtcc tgtccgttcg tgtgctcgac atacaggtcc tgggtatcgc
     3661 cgtagagcgc cgtgaatccc cacgcgatgt ggtcgttgtg tccggccacg atcagcggca
     3721 gccccggcac cgtcactccc gcagcgtgca gacctggcgc cttgagcccg gtcatgtacc
     3781 agatgttggg aatctgctgc gccaggtgca tgtcattgga gagaatcggg ctcccgctcg
     3841 ccgtccgcgc gcccgatacc acccactgat tcgatcccgg attcgtgtcg agtccgcatt
     3901 cgacgcatgg cggatggcct gtgagctgct ccagacgccg cagatcatcg tgcaggatcg
     3961 gcgagtcgag acgggccgcc ccaaacccat cctggctttc gtccagcgga acatcaggca
     4021 gattctgatt tggcatcgta atcggcacct catcggtcac cggcggatga tcgcgccacg
     4081 agcccgtggg ataaagatcg gccgcgagcg tcgggcccag ccgcgccgta atgcgctcgc
     4141 gctccagctt ctccggccag tgctcgtcca gcatctgcac catgctcatc accaccagca
     4201 tggagtccac cggctgccag ggcttcggtt gatagaagag caaacggaac tccgcgggca
     4261 gccgatccat gtgcgagccg atgtaggcgt tcacgccgcg tgcgtaatcg tcgaacagac
     4321 gccgctcgtc ggccggcagt gcggcggtga gccgctcagc ggtcacgcgg accgcgagca
     4381 tgcgctgcat gcggtcgtgc tccagcaggc gcgacccaag gatctctgcc gcctccccgg
     4441 acgccatgcg tcgcgccatg tccatctgcc acagacggtc ctgggcggtc acgtacccct
     4501 gagcgaaaag caggtcgtcg agattggcag cctcaatgtg cggaaccccg tgatggtcgc
     4561 ggcggacgat cacctgcgtc gtcagtcccg gggtcagcag cgcgccatcg atctccggca
     4621 gcgacgactg catcttgtgc ttgagccaga ttcccgccgt gaacaccgcg cccgccagga
     4681 caagcaggaa caggaagaac aggatcagaa cgacgcgcag gaagggatgg cggcgcttct
     4741 cctcgtgaat gtcaacactg cggccaagag gcatttccag atcagggtac agggtacagg
     4801 tcgcagggta cagggtacag gtcgcagggt acagccagtt gctctcaact cgctgttccc
     4861 tgctgcctga tgcctgttct ctactggcta ttggctactg gctactggcc attccctgtc
     4921 tgctgctgcg ggcggctcat cggcgggatg atgctgttca tgcgcagata ctccaccatc
     4981 tggccatagt gatccatcga gtgggcgatg caaaacgcag cgacggacgc tttcgtcatt
     5041 ttgaactgag gtggcagaaa gctttttacc ggatccccgg cgttctccgg ggtcagggtt
     5101 cccaccgcgg catgcgcata cttgtaggag tctttcagcg cctgcacgat ctcatccttg
     5161 gtcttcagcg cttcgatcgt ctttggatcg accgccgtgg cgcctgggac gttccagccg
     5221 ttgaagaact cgtagttgga ctcagccaga tgcttgacct gctgagcgaa ggtgcggacg
     5281 cctttgaagt cgcccatgga agcagg
//
LOCUS       DASZYN010000432         3029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_211791, whole genome shotgun sequence.
ACCESSION   DASZYN010000432 DASZYN010000000
VERSION     DASZYN010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211791"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..515
                     /locus_tag="VHE33_11175"
     CDS             171..515
                     /locus_tag="VHE33_11175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVT98059.1"
                     /translation="MEKRNGLLQGTLDMLILRTLRSGAAHGHEIGKHIQRTTNDFLQM
                     QHGSLYPALHRLEKRGWVTAKWETAPDRNREFKYYRLTAQGRKQLTVEESQWKQMAEA
                     VARVMWPVAEES"
     gene            517..>3029
                     /locus_tag="VHE33_11180"
     CDS             517..>3029
                     /locus_tag="VHE33_11180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT98060.1"
                     /translation="MQWWRLRKKDADLERELASDLELEEEEQRERGLSAEEARYAARR
                     AFGNPTLIREHTRATWNWGSVERWVRDLQHGARTLLRSPGFSLISVLVMALSIGATTS
                     LFTMVRAVLLRPLPFRDSGKLVMLYEHFRHNQGGDGFNAVAVGDYRDWRSQTHGFEDM
                     AAMRAYGGIVSGVHSELPEVVQSAGGSSNLFSLLGVAPAYGRVFTQAEDQPQGERVVL
                     LTWSFFQRRFAGDPSIVGKQVHLDTIPTTVVGVLPKWFTYPDARIQFWIPYAQTFLPD
                     EYGIHDGHQSMVVARLRPGVSEESATREVSALQYQIHLANAAKPVAEDVWSRPMIDDL
                     VRNVRRPLLVLLGAVGCMLLIACLNLTNLLVARSAAHRKEVAIRGALGGSRFVLIREQ
                     MMESLLICLSGGGLGLLLSLASTWWLAAHWRNLPRAESVHVDGWVLLFACGLVAATTL
                     LAGLVPAISSTGKGLLTGLREGSRSVGGNVSRARLRKTMLSAEIALTVMLLVSAGLLF
                     KSFVRLRTTDLGCRVDHVMTMKFGLPEIQYDTRDKVVTFHESLLERVRRLPGMRGAAL
                     VSNAPGTGPVGDKVFTILERPAPRYATQYDAATLMADPQYFSVMQIPLIRGRVFTEHE
                     RLRNDHYVVVSKLFAEQFFSGDDPVGKHIRLSWDTQLESYEILGEVGDTISDVTKPAQ
                     ATIYFPILSGIPDRTSEATIVAWTAGDPLAMSMPIQQQISALEPELPVYNALTMDQIL
                     GKTTASQGFAANLVLAFAGLALLLAAVGLYGVLSYLVTQRVSEIGIRVALGAQRVQVL
                     RLVLFDGLRPAFLGLMAGLAASAGVTQLIRSMLYGTEPY"
BASE COUNT          549 a          848 c         1025 g          607 t
ORIGIN      
        1 gcgcttgcca gaaattgccg tcgatccatg tcacgctcca gcctaattag gggatggttt
       61 catgctgcct ggaaccggtc aaggcgagcg gcggggtcag gaagactcgc ttgacgaatc
      121 gatagtcgag cgtctaccat cggcctcaca ttgagggtcg attgtcttct atggaaaaac
      181 gcaacggact tcttcagggc acgctggaca tgctcatcct gcgaacgctg cgttccggcg
      241 ccgctcatgg tcacgagatt ggaaagcaca ttcagcggac aacgaacgat tttctgcaga
      301 tgcagcatgg ttcgttgtat ccggcactgc atcggctgga gaaacgaggc tgggtgactg
      361 cgaaatggga gacggctccg gatcggaatc gggaattcaa gtactaccgt ttgacggcgc
      421 agggccggaa acagctgacg gttgaagaat cgcagtggaa gcagatggcc gaagctgtgg
      481 cgcgggtgat gtggccggtc gccgaggaga gctgagatgc aatggtggcg gctcagaaag
      541 aaagacgcgg atctggagcg ggagctcgcg tccgatctgg agttagaaga agaagagcag
      601 cgggagcgcg ggttatcggc ggaagaggcg cgctatgctg ctcggcgtgc gttcggcaat
      661 cccacgctga ttcgcgagca cacgcgagcg acctggaatt ggggttccgt ggagcgatgg
      721 gtgcgcgacc tgcaacacgg cgcgcgtact ttgctgcgct cgccggggtt ctcactcatc
      781 tccgtgctgg tgatggcgct cagcatcggc gcgaccacat cgctgttcac catggtgcgt
      841 gcggtgctgc tgcggcctct gccgtttcgc gattccggca aactggtgat gctctatgag
      901 cacttccggc acaaccaggg cggcgatggt ttcaatgcgg ttgccgttgg agactatcgg
      961 gactggcgaa gccagaccca cggctttgaa gacatggcgg cgatgcgcgc ctacggcggt
     1021 atcgtgtccg gcgtgcactc cgaactgccg gaagtggtgc agagcgcggg gggatcgtcg
     1081 aatctgtttt cgctgctggg cgtagctcca gcctatggcc gcgtgttcac gcaggccgaa
     1141 gaccagccgc aaggggaacg cgtggtcctg ctcacgtgga gctttttcca gcggcgcttt
     1201 gcgggagatc cttccatcgt cggtaagcag gtccatctgg atacgatccc gaccacggtt
     1261 gtgggcgtgc tgccgaagtg gtttacctat ccggatgcgc gcatccagtt ctggattcct
     1321 tacgcgcaga cgttcctgcc ggacgagtat ggcatccacg acgggcacca gagcatggtg
     1381 gtggcccggc tgcggccggg ggtgagcgaa gagtcggcga cgcgcgaggt gagcgcgctg
     1441 caataccaga ttcacctggc caatgcagca aaaccggttg ccgaggacgt ctggtcgcga
     1501 ccgatgatcg acgacctggt gaggaatgtg cgaaggccgc tgctcgtcct gctgggcgcg
     1561 gtggggtgca tgctgctgat cgcgtgcctg aatctgacga acctgctggt agcccggtcg
     1621 gctgcgcatc gcaaggaagt tgccatccgc ggcgcgttag gcggcagccg gtttgttctg
     1681 attcgcgaac aaatgatgga gagcctgctg atctgcctga gcggcggagg actgggcctg
     1741 ctgctgtcgc tcgcttcgac ctggtggctg gctgcacact ggcgcaatct gccgcgcgcg
     1801 gagtcggtcc atgtcgatgg atgggttctg ctgtttgcgt gcggcctggt ggcggccaca
     1861 acgctgctgg ccgggctggt gccggcaatt tcatccaccg gcaaagggct gctgaccggg
     1921 ttgcgggagg gatcgcgatc ggtgggcggc aacgtgtcgc gggcgcggct gcgcaagaca
     1981 atgctgtcgg ccgagattgc gctgacggtc atgctgctgg tgtccgccgg tctgcttttc
     2041 aaaagcttcg tgcgcctgcg gacgacagac ctcggctgcc gagtcgatca cgtgatgacg
     2101 atgaaattcg ggctgcccga aattcaatac gatacgcgcg acaaggttgt gaccttccac
     2161 gagtcgctgc tggaacgggt gcgccgactc cccggtatgc gcggcgccgc gctggtctct
     2221 aatgcgcccg gaacaggccc ggtgggcgac aaagtcttca cgatcctgga gcggcctgca
     2281 ccccgatatg ccactcaata cgatgccgcg acgctgatgg ccgatccgca gtacttcagc
     2341 gtgatgcaaa ttccgctgat ccgcggcagg gtcttcaccg aacacgagcg gctcagaaac
     2401 gaccattacg ttgtggtcag caagcttttt gccgagcagt ttttctctgg ggacgatccg
     2461 gttggcaaac atatccgcct gagctgggat acccaactgg agagttacga gattctcggt
     2521 gaagtcggcg acacgatctc cgatgtgacc aaaccggcgc aggcgacgat ctatttccca
     2581 atcctatccg gcattcccga ccgcaccagc gaggcgacga ttgtggcctg gacagcgggc
     2641 gatccgctgg cgatgtcgat gccgatccag caacaaatct cggcgctgga gccggagttg
     2701 ccggtttata acgcgctgac gatggatcag attctcggca agacgacggc gagccaggga
     2761 ttcgcagcga atctagtgct ggcctttgcg ggtttggcgc ttctgctggc cgctgtcggg
     2821 ctttacggag tgctctccta cctggtcacg caaagggtgt cggagatcgg tatccgcgtc
     2881 gccctcggcg cacaacgcgt gcaggtcctg cgtcttgtcc tgttcgatgg gctgcggccg
     2941 gcgttcctcg ggctgatggc tggtttggcg gccagcgcag gcgtgacaca actgatccgg
     3001 tcgatgctct acggaaccga accgtatga
//
LOCUS       DASZYN010000433         2074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_786696, whole genome shotgun sequence.
ACCESSION   DASZYN010000433 DASZYN010000000
VERSION     DASZYN010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786696"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="VHE33_11185"
     CDS             <1..1177
                     /locus_tag="VHE33_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98061.1"
                     /translation="ATAWELAQRTNDQELLEKAYAACGRWDEWLRRYRDTRGTGLCEG
                     FCTYDTGQDNSPRWKGLANRCPDSDARRCPDEPGMPRLCPDLSATVYGGRVALAAMAK
                     ALGKTDDASRWKEDAETIQGLIIEKLYDPVDGAFYDLDSDNKFVKVRSSTIMTVLGEH
                     VPEPGVFDDVWSRQVHNPKAFWTAYPFPSVAMNDPQFVRPIPRNSWGGATQALTALRA
                     PRWMEHYGKSAELGELMHAWIEALYRAKGDFRQQMDPETGEFTMADPAGYSPAALVCL
                     DFLWRLHGVRQQGELVEWNVRTPAEHGRSRFSRKIGSTAMELSYEGNHAEMRVGGERV
                     ARVSGTVRLLTKKDGSLDSALGVDSKEQQVTIDVPKSLKRGFKIAPNEQVILRASTS"
     gene            1209..>2074
                     /gene="tyrS"
                     /locus_tag="VHE33_11190"
     CDS             1209..>2074
                     /gene="tyrS"
                     /locus_tag="VHE33_11190"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HVT98062.1"
                     /translation="MSSFLPVDEQMDLLEKGAAEIIRVPELRERLEKSRQTGVPLRVK
                     AGFDPTAPDLHLGHTVLMRKLKHFQDLGHQVIFLVGDYTSLIGDPTGRNVTRKPLTRE
                     QIDENAKTYTAQVFRILDREKTEVRFNSEWLGKLSFEDIIRLAAKFTVSQMLERAEFH
                     KRFQEEQPISLHELLYPLAQGYDSVALRADVELGGTDQKFNLIAGREMQRDYGQPSQI
                     VLMTPIIEGLDGVQKMSKSLNNAIGIHEPAPEMYGKLMSINDELMWRYWTLLTDLRSS
                     EIEKMRADVGSGA"
BASE COUNT          408 a          545 c          767 g          354 t
ORIGIN      
        1 ggcgacggcg tgggaactgg cgcagaggac gaatgaccag gagttgctgg agaaggcgta
       61 cgccgcatgc gggcgctggg acgaatggct gcggcggtat cgcgacacgc gcggcacagg
      121 tttgtgcgag ggcttctgca cctacgacac gggacaggac aacagtccgc gctggaaagg
      181 actggcgaac cggtgcccgg acagcgatgc gcgcaggtgc ccggacgagc cggggatgcc
      241 gcgcttgtgt cccgatcttt cggcgacggt gtatggcggg cgcgtggcgc tggctgcgat
      301 ggccaaggcg ctgggcaaga ccgacgatgc aagccgatgg aaggaagatg ctgagacgat
      361 ccagggcttg atcatcgaga agctctacga cccggtggat ggggccttct acgacctcga
      421 cagcgacaac aagttcgtga aggttcgcag ctcgacgatc atgacggtgc tgggcgagca
      481 tgtgccggag ccgggtgtct ttgacgatgt gtggagccgg caggttcaca acccgaaggc
      541 gttctggacg gcgtatccgt ttccgtcggt agcgatgaac gatccgcagt ttgtgcggcc
      601 gattccgcgc aactcgtggg gtggagcgac gcaggcactg acggcgctgc gggcgccgcg
      661 ctggatggag cactacggca agagcgcgga gctgggcgaa ctgatgcatg cctggatcga
      721 ggcactgtat cgggcgaagg gcgatttccg gcagcagatg gatccggaga cgggcgagtt
      781 cacgatggcc gatccggcgg ggtattcgcc ggcggcgctg gtgtgcctcg acttcctgtg
      841 gaggctgcac ggcgtgcgac agcaaggaga actggtggag tggaacgtgc ggacacctgc
      901 ggagcacggg cgttcgcggt tttcgcggaa gatcggcagc acggcgatgg agctgagcta
      961 cgaggggaac catgcagaaa tgcgggttgg cggcgagcgg gtggcacggg tgtcgggcac
     1021 ggtgcggctg ctgacgaaga aggacgggag cctcgattcg gcgctcgggg tcgacagcaa
     1081 ggagcaacaa gtaacgatcg atgtcccgaa gtcgctgaag cgcggtttca agatcgcgcc
     1141 caacgagcag gtgattctgc gggcttcaac ttcgtgagat cacggcgaaa tttatactga
     1201 cctgaactat gtcctctttt cttcctgtcg atgagcagat ggatctgctg gaaaagggcg
     1261 cggcggagat tatccgcgtg ccggagctgc gcgagcggct tgaaaaatcg cgccagactg
     1321 gcgtgcccct gcgggtgaag gccgggttcg acccgaccgc gcccgatctg catcttggcc
     1381 atacggtgct gatgcgcaag ctgaagcact tccaggatct cgggcatcag gtcatcttcc
     1441 tggtcggcga ctatacgtcg ctgatcggcg atccgaccgg gcgcaacgtg acgcggaagc
     1501 cgctgacgcg ggagcagatc gacgagaacg cgaagactta caccgcgcag gtatttcgca
     1561 tcctcgaccg ggaaaagacc gaggtgcggt tcaactccga gtggctcggc aagctgagct
     1621 tcgaagacat catccggctg gcggcgaagt tcacggtgtc gcagatgctg gagcgggcgg
     1681 agttccacaa gcgattccag gaggagcagc cgatttcgct gcacgagctg ctgtatccgc
     1741 tggcgcaagg ttacgattcg gtggcgctcc gagccgatgt ggagttgggc ggcacggatc
     1801 agaagttcaa cctgattgcc gggcgcgaga tgcagcgcga ctatggccag ccgtcgcaga
     1861 tcgtgctgat gacgccgatc atcgaggggc tggacggcgt gcagaagatg tcgaagtcgc
     1921 tgaacaacgc gatcgggatc cacgagccgg cgccggagat gtacggcaag ctgatgtcga
     1981 tcaacgacga gctgatgtgg cggtactgga cgctgctgac ggatttgcgc tcgtcggaaa
     2041 ttgagaagat gcgggcggat gtggggtcgg gcgc
//
LOCUS       DASZYN010000434         2122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_661083, whole genome shotgun sequence.
ACCESSION   DASZYN010000434 DASZYN010000000
VERSION     DASZYN010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661083"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VHE33_11195"
     CDS             complement(<1..622)
                     /locus_tag="VHE33_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98063.1"
                     /translation="MPEPIAAEMQQVSQKPVYDLRKQQTLFSVLTLFVLAMLLLLHML
                     FAVVLGEPSVSVIVVLGLSFALRLAELGWLQGRGEPLSDFAAKLDGLVSIVSLFVLAV
                     LLAWLTDRDHSPYQVLLIIPVLQAATLFRLTPTLLTILAADATIFLWLWHYFALYPPA
                     LLDEYLEAGMFAVVVALVGVLMWVLMRILRSHQTALDRTLSDLQSAR"
     gene            complement(772..1308)
                     /locus_tag="VHE33_11200"
     CDS             complement(772..1308)
                     /locus_tag="VHE33_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98064.1"
                     /translation="MASDSIPSVAIFQPAVLSHLPLVLLLLCPVFILLGAALPPLRGW
                     PWRLAALLLLGIGTAGLFFAIPGPQHYDPRLMPNPAAGAVQWIYRSLVMEARIIFMGL
                     TAIYVGVILLPEVLRRRDNRLFSTVLPLSFLLLYSAGAVFLMQTTDSAAVVTQTLGIG
                     ADDTGASHRAEAPVSERK"
     gene            1666..>2122
                     /locus_tag="VHE33_11205"
     CDS             1666..>2122
                     /locus_tag="VHE33_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT98065.1"
                     /translation="MTQICLESIGSYALEETAQRRQQDAEDQRRRRRILCVDDDPVVL
                     SLQRALLESAGFSVITARDGKEGLRRFSAEFPDAAVLDYAMPGMNGAALAAQMRRIAE
                     DVPLILNSGSVTVSAEEARLFERVLSKGLAPGLLVRVLREIFPLLGAGAP"
BASE COUNT          456 a          585 c          720 g          361 t
ORIGIN      
        1 ctcgggcgct ttgcaggtcg gacaaggtgc ggtccagcgc agtctgatga gagcggagga
       61 tccgcatgag cacccacatg aggacgccga ccagagccac cactacggcg aacatgccgg
      121 cctcgagata ttcgtcgagg agcgcgggcg gatagagcgc gaaatagtgc cacagccaga
      181 ggaagatggt ggcgtcggcg gcaaggatgg tcagcagtgt gggcgtcagg cggaacaggg
      241 ttgctgcctg gaggacggga atgatgagca gaacctggta agggctgtga tcgcgatccg
      301 tcagccacgc cagcagtacc gctaacacga agaggctgac gatggaaacg aggccgtcga
      361 gctttgcggc aaagtcgctg agaggttcgc cgcggccctg cagccagccg agttcggcca
      421 gacgcagagc aaaactcagt ccaagaacaa caatgacgga gacgctcggc tccccgagca
      481 cgaccgcgaa gagcatatgc aggagcagca gcatggcgag cacaaaaagt gtgagaacag
      541 aaaagagggt ttgctgcttg cgcagatcgt aaaccggctt ttggctcacc tgttgcattt
      601 cggctgcgat gggttcgggc atcggttgga tccgccggcg tgacggcact gcttcgcctt
      661 gctcgcaatc cctggaatga tccgggatac gaacgatggg agcattccgg cgcatcaggc
      721 gatttgtgat caggttacgc cctgaggaag gaaggacacc gaaattgggt atcactttcg
      781 ttccgagacc ggcgcctcgg cgcgatgaga ggcaccggta tcgtcggcgc caatcccgag
      841 ggtctgcgtc accacggctg ccgaatcggt ggtctgcatc aggaacacgg caccggctga
      901 atagagcaga agaaaggaca agggcagcac ggtggagaaa agccgattgt cgcgacgccg
      961 caagacttca ggcagcagga tgactccgac atagatggcg gtgagaccca taaaaatgat
     1021 gcgggcctcc atgacgaggc tgcggtagat ccactggact gcgccggcgg cgggatttgg
     1081 catgagtcgc ggatcgtaat gttgcgggcc cggtatcgcg aagaacaggc cggcggttcc
     1141 gatgcccagc agcagcagag ccgccagacg ccagggccat ccgcgaagag gggggagggc
     1201 ggctccaagg aggatgaaga cgggacagag cagcagcaga accaaaggaa gatgactcag
     1261 cactgcaggc tggaatattg cgaccgacgg gattgagtca ctcgccatga ccggcctcct
     1321 ggtgctgcgt cgccgtcagg ggcagcgcgg cgccatacga cccttcgcgc tgcgctgccc
     1381 ctgcgtttcg caggatccac ctcctcccaa tggcatttgg aaggccattc ttgttcctgc
     1441 gaaacaatcc atgtctctgc gcctggtcct gatttcggac gggtttgtcc gaaagggcgg
     1501 acaccggcgt cggaatattc cgacacgcag ggttgcgcag aacgaaagct gttgagaaaa
     1561 cgctgcaacc gggcggcttg tcctggaatg ccacatgcaa cgtggaaagc gccgggttgt
     1621 tgccatggcc tgacacatgg cagtaatccg gcggagaggg attttgtgac tcagatctgc
     1681 cttgaatcga tcggatcgta tgcactggaa gagaccgcgc aacgtcgtca acaagacgcg
     1741 gaagaccagc gccgccggcg aagaattctt tgcgtcgacg acgatccggt cgtcctctct
     1801 ctgcagcgag ctttgctgga gtcggccgga ttctcggtga tcacggcacg ggacgggaag
     1861 gagggcctgc gcaggttttc cgccgaattc cccgacgcgg cggtgctgga ctatgcgatg
     1921 ccgggcatga acggcgccgc tctggcggca cagatgcgcc ggattgccga agatgttccg
     1981 ctcattctga attccggatc cgtgacggtg tccgcggaag aggccaggct cttcgagcgg
     2041 gtcctgtcga agggattggc tccgggtctg ctggttcgcg tgctgcgcga gatctttccg
     2101 ctgctcgggg ccggagcccc gg
//
LOCUS       DASZYN010000435         4728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_21591, whole genome shotgun sequence.
ACCESSION   DASZYN010000435 DASZYN010000000
VERSION     DASZYN010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21591"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="VHE33_11210"
     CDS             complement(<1..1205)
                     /locus_tag="VHE33_11210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HVT98066.1"
                     /translation="MMRFRPWGVASLAAFLLAGCTVGPNYHRPDAATAPAWKENAPPP
                     PNPPNGTWKQAEPNDGVLRGAWWEMYGDPQLNALEAKVAVSNQTLRAAMEQYFSAREQ
                     VRVARSRYYPTLSAGPSAQRTRESLNQANTNRTVTNYQFNTFTIAGQAQWEPDLWGQV
                     RRTVEQARANAQASAALLANVELSLRSELASDYFEMRGLDSQQQLLDTTVKSFEEYLK
                     LTQIRFKGGVATESDVALAQTQLETTRAQDIDVQVARAQFEHAIATLTGTPASSFSLA
                     AMPLGDQIPQIPVGVPSQMLERRPDIAAAERQADAANAQIGIAIAAYYPNVSLTGSGG
                     FSSGRPGTLIQGPSEVWALGASAVELLFDAGRRHAITQQARDAYEQQVANYRQTVLAA
                     FQDVEDNLSA"
     gene            complement(1202..2476)
                     /locus_tag="VHE33_11215"
     CDS             complement(1202..2476)
                     /locus_tag="VHE33_11215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVT98067.1"
                     /translation="MSEPKPTNAHNGDTHGYVDDRPPISGGKALVWIIVLAAIAAVVA
                     IAGILPRVRAQKTLRERTDSLALANVTVEKPQLGAPTEELVLPGNMFAYVDSPIYART
                     DGYLEKWYFDIGAHVRKGQLLATIASPEVDQQLAQAKAELATAESTAGYAKQQASRYT
                     DLLANNAVAKQDTENFVTQAASTNMQVKSAQANVQRLEQLTGFEKVYAPFDGILTARD
                     VDIGTLINAGAGGTGGREMFHMADEHIMRVYVNVPQVDSPECTPGTPADLTLEQYPGR
                     RFHGKVVRTSRAIDPASRTLLVEVDVPNAKGELVPGAYTQVHFQLKVMKQTLILPVSA
                     LLFRQEGLRVVTVVHGSGGDVAKLLPITIGQDDGRVVQVTEGLNANDEIVRNPPDSII
                     DGEKVRVVSPNGGPGGERQEENQGNDGPEGGA"
     gene            complement(2473..>4728)
                     /locus_tag="VHE33_11220"
     CDS             complement(2473..>4728)
                     /locus_tag="VHE33_11220"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVT98068.1"
                     /translation="IMILIFLGSWRSTLIIAVSIPLSILTSLLVLSALGESINIMTLG
                     GLALAVGILVDDATVEIENINRNLEQGKAIEQAILDGAAQIAVPALIATLSICIVFVP
                     MFFLSGVARYLFVPLAEAVVFAMLASYLLSRTVVPTMAKYLLREHDPQEGERKLLSRN
                     PFIVFQLAFESRFEKIRRSYRRMLELCIAHSALFIVLFFVLSMGSAALLYPFLGEDFF
                     PNVDAGQFKLHVRARTGTRIEDMAALCDRIDNSIRQVIPKDELVTIIDNIGLPYSGIN
                     TSYSNSAPVGPADADISVSLSEHHRPTEEYVARLREELPRRFPGTEFYTLPVDMVTQI
                     LNFGLPAPIDIQIVGHNQQANFVFAEHLLNQVKYVPGAADMHIQQSFDNPSLTVNVNR
                     SMAQSAGYAQRDVMQSLLVASSGSFQTSPTFYLDPQNGVEYSIAAQAPQYSLSTMQDL
                     RNIPVTSAGGGAGASPLSSASLGAGMPSSAGGPVGGKPVQILGNLASIVPGTEPAIVN
                     HYNITPVIDIFGNVVGTDLASVSKGIQEKVDQQKKYLPHGSHIEVRGQIDTMRRSFEG
                     LLAGLVFSIVLVYLLIVVNFQSWLDPFIIVCALPAAISGIVWFLFLTHTRLSVPALTG
                     AIMCMGVATSNSILVISFAREQLEVTVGDASLSALNAGFVRFRPVLMTALAMILGMIP
                     MSLGLGSGGEQNAPLGRAVIGGLLFATVSTLFFVPTIFSVLHGRRERRRREREARQHA
                     ERRQLLTGSRP"
BASE COUNT          924 a         1675 c         1253 g          876 t
ORIGIN      
        1 gctgacagat tatcctcgac atcctggaac gccgccaaaa ccgtttgccg atagttggcc
       61 acctgctgct catacgcgtc tcgggcctgt tgcgtaatcg catgccggcg tcccgcgtcg
      121 aacaacagtt ccaccgccga cgcccccagc gcccacactt cgctcggccc ctgaatcagc
      181 gtgcccggcc ggccactcga aaatccgccg ctgcccgtca aagaaacatt tggataatac
      241 gcagcaatcg caatcccaat ctgcgcgttc gcagcatccg cctgccgttc ggccgcggcg
      301 atatccggtc tccgctccag catctgtgac ggcacgccca ccgggatctg cggaatctga
      361 tcccccaacg gcatcgccgc gaggctgaag ctcgaagccg gcgttcccgt cagcgttgcg
      421 atggcgtgtt cgaactgcgc tcgagccacc tggacgtcga tatcctgtgc ccgcgtggtc
      481 tccagctgcg tctgcgccag cgccacatcc gactccgtcg ccactccgcc cttaaaacgg
      541 atctgtgtca gtttcaaata ctcttcgaac gacttcaccg tggtgtcgag cagttgctgc
      601 tgcgagtcca gcccgcgcat ttcgaagtag tccgaggcca gctccgaccg caggctcagt
      661 tccacattgg ccagcagcgc cgccgaagcc tgcgcgttgg cgcgcgcctg ttccacggtc
      721 cgacgcacct ggccccacag atcgggctcc cactgcgcct gccccgcgat cgtgaacgtg
      781 ttgaactgat aatttgtaac cgtgcgattg gtatttgcct ggttgagcga ctcgcgcgtg
      841 cgctgtgccg acggtcccgc cgacaacgtc gggtagtacc gggaacgcgc cacccgcacc
      901 tgctcgcgcg ccgagaaata ctgctccatc gccgcgcgca gcgtttggtt cgatacagcg
      961 accttcgcct ccagcgcgtt gagctgcgga tcgccataca tctcccacca cgccccgcgc
     1021 agcacaccat cgttcggctc cgcctgcttc cacgttccgt tcggcggatt cggaggtggc
     1081 ggcgcattct ctttccatgc cggagccgtc gcggcgtccg gtcgatggta gttcggcccc
     1141 accgtacaac ccgcaagcag gaacgcggcc agacttgcca ccccccatgg gcggaatcgc
     1201 atcatgcccc gccctccggc ccgtcgttgc cctgattctc ttcctgccgc tctccgccgg
     1261 ggcctccgtt ggggctcacc acccgtacct tctcgccatc gatgatcgag tccggcgggt
     1321 tccgcacgat ctcgtcgttg gcgttcagac cctcggtcac ctgcaccacg cggccgtcat
     1381 cctggccaat cgtgatcggc agcagcttcg ccacatcccc gccgctgcca tggaccaccg
     1441 tcaccacccg cagcccttcc tgtcgaaaca gcagcgccga cacaggcagg atcagagtct
     1501 gcttcatgac cttgagctgg aaatgaacct gcgtatatgc gcccggcacc agttcgccct
     1561 tggcattcgg cacatccacc tcaaccagca gcgtccgcga tgccggatcg atcgctcgcg
     1621 aggtgcgcac cactttgccg tggaaccgcc gcccaggata ttgctccagc gtcaggtcgg
     1681 ccggagttcc cggtgtacac tccggcgaat ccacctgcgg cacgttcacg tacacacgca
     1741 tgatgtgctc atcggccatg tggaacatct cgcgtccgcc cgtccccccg gcccctgcat
     1801 tgatgagcgt cccgatatcc acatcacgcg ccgtcaaaat cccatcgaac ggagcataga
     1861 ctttctcgaa tcccgtcagc tgctccaaac gctggacatt tgcctgcgcc gacttcacct
     1921 gcatgttcgt cgatgcggcc tgcgtgacga aattctccgt gtcctgcttt gctaccgcgt
     1981 tgtttgccag cagatcggta tagcgcgacg cctgctgctt cgcgtagcca gcggtcgatt
     2041 ccgccgtcgc cagttcagcc ttcgcctgcg ccagttgctg atccacctcc ggactcgcaa
     2101 tcgtcgccag cagctgtccc tttctcacat gcgcgccaat gtcgaagtac cacttctcca
     2161 gatagccatc cgtccgcgca tagatcggcg aatcgacgta cgcaaacatg ttgcccggca
     2221 gcacgagttc ctccgtcggt gcaccgagtt gcggcttctc caccgtcaca ttggccaggg
     2281 ccagggaatc cgtccgctca cgcagcgtct tttgcgcgcg cacccgcggc agtatgccgg
     2341 caatcgcaac caccgccgca atcgccgcca gaacgatgat ccataccagc gccttccccc
     2401 ccgagatcgg cggacggtca tcgacatacc cgtgcgtatc gccgttgtgc gcattcgtcg
     2461 gtttgggttc gctcatggcc tgctccccgt caaaagctgt cgcctctcgg cgtgttggcg
     2521 cgcctcccgc tctcggcggc ggcgctcgcg gcgtccgtgc agcacgctga aaatcgtcgg
     2581 aacaaaaaac aacgttgaaa ccgtcgcaaa cagcagaccg ccaatcacgg cgcggcccag
     2641 cggcgcattc tgttcgcccc ccgaacccaa tccaagcgac atgggaatca tgcccagaat
     2701 catcgccagc gcagtcatca gcaccggccg gaatcgcaca aacccggcat tcagagccga
     2761 cagcgatgcg tcacccaccg tcacctcgag ttgctcccgc gcaaaactga tcaccagaat
     2821 cgaattcgac gtcgccacgc ccatgcacat gatggccccc gtcagggccg gaacactcag
     2881 ccgcgtgtgg gtgaggaaaa ggaaccacac aatgccggaa atcgctgccg gcagcgcgca
     2941 aacgatgatg aacggatcga gccacgactg gaaattcacc acaatgagca ggtacaccag
     3001 cacaatcgag aacaccaggc cggccagcag gccctcgaac gagcgccgca tcgtgtcgat
     3061 ctgccctcgc acctcgatgt gcgagccatg cggcagatat tttttctgct gatccacttt
     3121 ctcctgaatc cccttgctca cactggccag atcggtcccc accacgttgc cgaagatgtc
     3181 gatcaccggg gtgatgttgt agtgattcac aatcgcaggc tcggtccccg gcacaatcga
     3241 cgccagattg cccaggatct gtaccggctt cccgcccacc ggccctcccg cggatgacgg
     3301 catgcccgcg cccagcgatg cgctgctcag cggcgatgcc cctgccccgc cgcccgccga
     3361 tgttaccgga atattgcgca ggtcctgcat cgtcgacagc gaatactgcg gcgcctgggc
     3421 cgcgatgctg tattccacgc cgttctgcgg atccagataa aacgtcggtg atgtctgaaa
     3481 gctgccgctg ctcgctacca gcagactttg catcacatcc cgctgcgcat atccggcact
     3541 ctgcgccatc gagcgattca cattcacggt caggctcgga ttatcgaagc tctgctgaat
     3601 atgcatgtcc gccgcgccag gcacatactt cacctggttg agcagatgtt cggcaaatac
     3661 gaagttcgcc tgctgattgt gccccacaat ctgaatatca atcggcgccg gcagaccgaa
     3721 gttcaggatc tgcgtcacca tgtccaccgg gagtgtgtag aactccgtac ccggaaaacg
     3781 ccgcggcaac tcctcgcgca gccgcgccac atactcctcc gtcggacggt gatgctcgct
     3841 cagcgacacc gaaatatccg catccgccgg ccctaccggg gccgaattcg aatacgacgt
     3901 gttgatgccg gaatagggca ggccgatgtt gtcgataatc gtgaccaatt cgtccttcgg
     3961 aatcacctgg cgaattgaat tgtcgatcct gtcgcacaac gcggccatat cctcaatgcg
     4021 cgtccccgtc cgcgcgcgca cgtgcagctt gaactgcccg gcgtccacgt tcggaaagaa
     4081 atcctcgccc agaaagggat acagcagtgc tgccgaaccc atgctcagca cgaagaacag
     4141 cacaatgaac aatgccgagt gggcgatgca gagctccagc atccgccggt agctgcgccg
     4201 gatcttctca aagcggctct caaacgccag ttggaacaca atgaacggat tccggctcag
     4261 cagcttccgt tcaccctcct gcgggtcatg ctcgcgcagc agatacttcg ccatcgtcgg
     4321 cacgacggtc cgcgaaagca aatacgacgc cagcatcgca aacaccaccg cctcggccag
     4381 aggcacgaag agataccgcg ccaccccgct caaaaagaac atgggcacga aaacgatgca
     4441 gatcgagagc gtggcgatca gtgccggtac cgcgatctgc gccgcaccat cgagaatcgc
     4501 ctgctcgatt gccttgccct gctccagatt ccgattgatg ttctcgatct ccacggtggc
     4561 atcatcgacc agaatgccca ccgccagagc caggccgccg agcgtcatga tgttgatcga
     4621 ctcacccagc gcgctgagca ccagcagcga cgtcaaaatc gacaacggga tcgaaaccgc
     4681 gataatcagc gtgctccgcc agctgcccag gaagatcaga atcatgat
//
LOCUS       DASZYN010000436         2911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_853459, whole genome shotgun sequence.
ACCESSION   DASZYN010000436 DASZYN010000000
VERSION     DASZYN010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853459"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VHE33_11225"
     CDS             <1..518
                     /locus_tag="VHE33_11225"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVT98069.1"
                     /translation="WNIAPLFAGGTKPSRVEVISGANVMFRREVFAQIGQFSEEYFMY
                     AEDLDLCYKAVQAGYTNYYIPQAQIIHLGGKSSVKRRAVVMKWRSILQYVAKHRGAAY
                     QLAFRAVMGCSALARLALLLMAVAVSRGSRRNSAREAMLKWWLVLETMVSRIEKKQRP
                     DKLAAEGAPGN"
     gene            557..2470
                     /gene="asnB"
                     /locus_tag="VHE33_11230"
     CDS             557..2470
                     /gene="asnB"
                     /locus_tag="VHE33_11230"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HVT98070.1"
                     /translation="MCGICGKFNFDRARPVSSGLLKRMAGTIAHRGPDDEGYHLSGPV
                     GLGFRRLSIIDLATGHQPLSNEDGTVWIVFNGEIYNFEELRNDLLARGHLFRTRTDTE
                     VIVHLYEEYGPACVEKLRGMFGFAIWDERQQTLMLARDRVGIKPLYYAMGKDSLVFGS
                     EIKAILADPEISARVRPEIVDRFLTFYFVPGEDTLFENVLKLRPGCYMTVRAGKAEVH
                     QYWDLTFNPTPKSAADAERELVELLDECVRLHMISDVPVGVLLSGGVDSTAMVSFTSN
                     KTDHRLGSFTLGFGGTEVPDERPYARMAAERYGTNHHEMTISAEDFRDFLPNYAWYME
                     EPVCEAPAVALYYITKLARESVTVLISGEGGDEAFAGYQTYRGVQWVERLKRALGPLK
                     GPLSSAALGANGLLKNPRIAKYAPLFGQSFEDYYYSRSSGPDSFFNRNVGTLYSSDFA
                     GISDKSFATRPMKEIMASSRGVGLVNRMLYVDTKSSLPDDLLLKADKMTMANSIELRV
                     PFLDHKLLEFAASLPESLKVHGLTTKYIAKRALSNRIPEPILKRRKAGFPVPFGAWMR
                     KELKPFVHEVLLDRTTIERGYFNTASVENLLRANEESGRYSKEVLSLLTLELWHRAFL
                     TSNTGVSPEPVGV"
     gene            2529..>2911
                     /locus_tag="VHE33_11235"
     CDS             2529..>2911
                     /locus_tag="VHE33_11235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT98071.1"
                     /translation="MKTAKYVIISPVRDEEVHLSRTIESVVHQSVRPVEWIIVNDGST
                     DRTAEIADNYAREHEWIRVVHRDNRGFRKSGGGVVDAFNDGYRNLKTRDWEFIVKLDG
                     DLSFDSEYFESCLRAFAQEEKLGIAG"
BASE COUNT          608 a          758 c          922 g          623 t
ORIGIN      
        1 tgtggaatat cgcgccgctg ttcgcgggcg gcacgaagcc gagccgggtg gaagtgattt
       61 ccggcgcgaa cgtcatgttc aggcgagaag tgttcgcgca gatcgggcag ttcagcgaag
      121 agtatttcat gtacgcggag gatctggacc tctgttacaa ggcggtgcag gctggttaca
      181 cgaattacta cattcctcag gcgcaaatca ttcatctcgg cggcaagagc agtgttaagc
      241 gacgggccgt ggtgatgaag tggagatcga ttctgcagta tgtggccaag catcgcggcg
      301 ccgcctatca gctggcattt cgtgcggtga tggggtgcag cgcgctggca cgcctggctc
      361 tgttgctgat ggccgtcgcc gtaagccgtg gctctcgcag aaactcggcc cgtgaggcga
      421 tgttgaagtg gtggctggtg ctggagacga tggtgagccg tatcgagaaa aagcagcggc
      481 cggataagtt ggccgctgag ggagcgccgg gaaattagcc aggcgcctgc aatgatttta
      541 ttggcgagaa cattctatgt gcggaatttg cggtaagttc aatttcgatc gagccaggcc
      601 ggtttcgagc ggcttgttga agcgtatggc gggtacgatc gcccatcgcg gacccgacga
      661 tgaaggctac cacctgtccg gtcccgtggg tctcgggttc cggcgccttt ccatcatcga
      721 tctggcaacg ggtcatcagc cgctgtcgaa cgaggacggg acggtgtgga tcgtcttcaa
      781 cggcgagatt tataacttcg aggaactccg caacgatctt ttagcgcgcg gtcatctgtt
      841 ccggacgcgc accgataccg aggtgattgt tcacctctat gaggaatacg ggccggcgtg
      901 cgttgagaag ctgcggggga tgttcggctt tgccatttgg gacgagcgcc agcagacact
      961 gatgctggcg agagaccggg tcggtatcaa acctctctac tatgcgatgg ggaaggactc
     1021 gctggtcttt gggtcggaga tcaaggcgat tctggctgat ccggagatca gcgcgcgagt
     1081 gcggccggag atcgtcgatc gtttcctgac tttttatttc gttcccggcg aagacactct
     1141 tttcgaaaac gttctgaagc tgagacccgg atgctacatg acggtccgag cgggcaaggc
     1201 ggaggttcat cagtactggg atctgacctt caaccccacg ccgaaatctg ccgcggatgc
     1261 ggagagggaa ctggtggagc tgctggatga atgcgtccgg ctgcacatga tcagcgatgt
     1321 gccggtgggc gtgctgctga gcggcggcgt ggattccacg gcgatggtca gtttcacaag
     1381 caataaaacg gatcaccggc tgggcagttt caccctcgga tttggcggga cggaagttcc
     1441 cgatgagcgc ccctacgcgc gaatggccgc ggagcgttat gggaccaatc accatgaaat
     1501 gacgatctcg gcggaagatt tcagggactt cctgcccaac tacgcgtggt acatggaaga
     1561 gccggtatgc gaggcgccgg cggtcgcgct ctactacatc acgaagctgg ctcgcgaatc
     1621 ggttacggtt ttgatttccg gtgaaggagg cgatgaagcc tttgccgggt atcagactta
     1681 tcgaggcgtc cagtgggtgg agcgcctgaa gagagctctt gggccgctga aggggccgct
     1741 ctcgtcggcc gccctcggag ccaacgggct tctgaagaat ccgcgcattg caaagtacgc
     1801 gccgctcttc gggcaatcat tcgaggatta ctactacagc cgcagttcag ggccggattc
     1861 cttctttaac cggaatgtgg gcacgctcta ctcgagcgat tttgccggta tcagcgataa
     1921 gtcgttcgcc acccgcccca tgaaagaaat catggccagc tcacggggtg tggggcttgt
     1981 gaaccggatg ctctatgtgg acacgaagtc atcgcttcct gacgatttgc tgctcaaggc
     2041 agacaagatg acgatggcga actcaatcga gttgcgcgtt ccatttctcg atcacaagct
     2101 gctggaattt gccgcttcgt tgccggaaag cctcaaggtc cacgggctga caacgaaata
     2161 tatcgccaag cgcgctcttt cgaaccgcat tccggagccg atcctgaagc gccggaaggc
     2221 gggattcccg gtaccgttcg gcgcctggat gcgaaaagag ctcaagccgt tcgttcatga
     2281 ggtgctgctg gatcggacga cgatcgagcg cgggtacttc aacaccgctt cggtcgaaaa
     2341 tctgctgcgg gcgaacgaag aatccggccg ctattccaaa gaagtgctgt ccctgctgac
     2401 gctggaattg tggcaccggg ctttcctgac cagcaacaca ggagttagtc cggaacctgt
     2461 gggcgtatag acggcgtcat tcaggaacct atgtgattcg aacaaccatg ccaaccccag
     2521 atatgtcatt gaagacagca aaatacgtca tcatctctcc ggtccgggat gaagaagtcc
     2581 acctttcccg aaccatcgag agcgtagtgc atcagtccgt gcggccggtc gaatggatca
     2641 ttgtgaacga tggatccacg gacaggaccg ccgagattgc cgacaactat gcccgcgagc
     2701 acgaatggat cagggttgtg caccgggata atcgtggctt ccgcaaatcc ggcggcggcg
     2761 tggtggatgc attcaacgat ggctatcgca atctgaagac ccgagactgg gaatttatcg
     2821 ttaagctgga cggcgatctt tccttcgatt ctgagtactt cgaatcgtgt cttcgcgcgt
     2881 ttgcacagga ggagaagctc ggcatcgcgg g
//
LOCUS       DASZYN010000437         3845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_150161, whole genome shotgun sequence.
ACCESSION   DASZYN010000437 DASZYN010000000
VERSION     DASZYN010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150161"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..645
                     /locus_tag="VHE33_11240"
     CDS             199..645
                     /locus_tag="VHE33_11240"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT98072.1"
                     /translation="MSSVQVEQKSYEMPPRDGISIAHFLTVADIERSARYYEKVFDAR
                     ILSMGDGNAPAYLQLANLWMILNVGGGPTPDKPTVTLSVPDPNHINSFMNFRVADIQA
                     CYQQWKSRGAEFITEPIPKYGEIRCYIRDPDGYIIEVGQSTDLTYG"
     gene            893..1096
                     /locus_tag="VHE33_11245"
     CDS             893..1096
                     /locus_tag="VHE33_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98073.1"
                     /translation="MFAWFSFKKPHIRLHVRPPVLQDHAKEVVPYPTTKAVVNFRMDN
                     PIPVTLTKKLVKASVKAMKDKAK"
     gene            1375..3009
                     /locus_tag="VHE33_11250"
     CDS             1375..3009
                     /locus_tag="VHE33_11250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HVT98074.1"
                     /translation="MGAVLEPPVAITADAITPPRPPSSADAAAVPAANPWIVAISVML
                     ATFMEVLDTSIASVALPYISGTLAASNSEATWVLTSYLIANAVVLPASNWFSLRFGRK
                     RFLITCVCLFTVASFWCGAAPSLGMLLLARVLQGAGGGALQPLSQAILLESFPPKKRA
                     AAMALFGFGVVVAPVIGPTLGGWLTDSFSWRYAFYINIPIGVLAVFMISRFVHDPPYI
                     HRSNAGAFDNLGFGLLCLWSGSLQIILDKGQEVDWFSAPWLRWATAILIVSLIWFIRH
                     EWTTKNPLVDLRILAQNRNFLVGCMLIFMLGFAIYITIAMMPLFYQEVLGYTALTAGL
                     VVAPRGLGSMLGLPLMGFISHRIDNRWLITIGFTGFGLLSIWFSLINTGIGPTTMLIP
                     IVLTGFALSFVFVPIGNMATVTLSNEQMGNATGIFNLLRNIGGSIGISLASTMLIRRM
                     AFHQSHISGTLPLTGVWFQQHMAAMAAYFAHQLGPAQGQPAALAAMYMQLERQALLWG
                     FVDVFRWTALVCFAAGLAVWSFRRISHGDDGSVKVH"
     gene            complement(3073..3309)
                     /locus_tag="VHE33_11255"
     CDS             complement(3073..3309)
                     /locus_tag="VHE33_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98075.1"
                     /translation="MAMSQLQLWVVIAAPSILALIGILLNRSGLHRLDGRIDRVEQRI
                     DQMMDIWNRDVKDLLGMISDHGQRIVRLEEHRRS"
     gene            complement(3380..>3845)
                     /locus_tag="VHE33_11260"
     CDS             complement(3380..>3845)
                     /locus_tag="VHE33_11260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HVT98076.1"
                     /translation="SEILLRLFRYAEDVERAVVMVQREVADRLAAVPGTRDYGVLTVT
                     AQMYARVEKLMTLPPGAFSPPPQVHSTVVRMTMGPRFGELGVEAEAFLQFVRSCFAQK
                     RKMLGKNLRNAGFEAGRVAAAMEKCGVSAGARAEELGVEVLAGLWRELRDRA"
BASE COUNT          759 a         1425 c          936 g          725 t
ORIGIN      
        1 gctcacctgg atcgaagcca acggacagcc ctcggtcctg atcgaacgcg agaacatgcc
       61 cattgccgtc gccaccatcg acgcctcgcc gcagggcatt caccaaatta tgtggatgat
      121 gaagccggac aaactcgcag ccatccgcag tacgaacaaa ccagcggtag cgtgaccaat
      181 ctcactattg gagccgtcat gagcagcgta caagtcgagc agaaaagtta tgaaatgccc
      241 ccgcgcgacg gcatcagtat cgcgcacttt ctcactgtgg ctgacatcga gcgatccgcc
      301 cgctactacg aaaaagtctt cgacgcccgc attctgagca tgggggacgg aaacgcgcct
      361 gcctaccttc agctggcgaa tctctggatg attctcaacg ttggaggcgg ccccaccccc
      421 gataagccga ccgtcaccct cagcgtccct gacccgaatc acatcaacag ctttatgaat
      481 ttccgcgttg cggatattca ggcctgctat cagcaatgga agagtcgagg cgcggagttc
      541 atcaccgagc ccatccccaa gtacggcgag attcgctgtt acatccgcga ccctgacgga
      601 tacatcatcg aagtagggca gagcaccgat ctcacctacg gctgacacgc gactccaaag
      661 agccgactcc atctgtgtcc cgccagtaga attggcggga taggggaggg cccatggccg
      721 ccaggagcaa agcgaacgaa gtcgacaagt acatcgccgg ctgtcctaaa gaggcgcagg
      781 ccaacctcgc gaagatcaga gccgcgatcc aggcggcagc cctggcgcca ccgaacgcac
      841 cgattatttc cagtggcctg gttactccta tcccggattt gactacgacg ggatgttcgc
      901 ctggttcagc ttcaaaaagc cgcacattcg gctccatgtg cgcccgcccg tgctccagga
      961 ccacgcaaag gaagtggtac cgtatcccac cacaaaagca gtcgtcaatt tccgcatgga
     1021 caacccaatt ccggtgacac tgacaaagaa actggtgaag gcaagcgtga aagccatgaa
     1081 ggacaaggcg aagtagggtt ctcctggcca tcggagcagt cacttacgtg cacatcggca
     1141 ggcccaggcg cacattaaag ccggcgcctt ccgatggatc ccacaaagtg ccgacatcct
     1201 ctcgcacatc cttcaagacc tgggttgatc agagattcct tacgcgttta gatgacatcc
     1261 ttgcccttga ggccgacgcc cctctgctgg ccgcatcccc accccctgtg acccacacca
     1321 cagccccgcc acccctccgc cccctaccct gaaaacatcg caggaagggc ctgagtgggc
     1381 gccgtactcg aaccacccgt agcgatcacc gcggatgcca tcaccccccc gcgcccgcct
     1441 tcctccgctg acgccgccgc cgttcccgcc gccaatccct ggatcgtggc aatctccgtc
     1501 atgctggcca ccttcatgga ggtcctcgac acctccatcg cctcggtcgc cctgccttat
     1561 atatccggca ccctcgcggc ctccaattcc gaagccacct gggtcctcac cagctaccta
     1621 atcgccaacg ccgtcgtgct gccggcaagc aactggttct cgctccgctt cggacgcaag
     1681 cgcttcctta ttacctgcgt ctgccttttc accgtcgcct ccttctggtg cggagccgcg
     1741 ccctccctcg gcatgttgct cctcgcccgc gtcctccagg gagccggcgg cggagcgctg
     1801 cagcccctct ctcaggccat cctgctcgaa agctttccgc caaaaaagcg cgccgctgcg
     1861 atggccctgt ttggcttcgg cgtcgtcgtc gcccccgtca tcggtccaac gctcggtggc
     1921 tggctcaccg attccttcag ctggcgctac gccttctaca tcaacatccc catcggtgtg
     1981 ctggccgtct tcatgatcag ccgcttcgtc cacgatccgc cctacatcca ccgcagcaac
     2041 gccggcgcgt tcgacaacct cggcttcggc ctcctctgcc tgtggagcgg atccctgcag
     2101 atcattctcg acaaaggcca ggaggtcgac tggttcagcg ccccgtggtt gcgctgggcc
     2161 actgcgatcc tcatcgtttc gctgatctgg ttcatccgcc acgaatggac gaccaaaaat
     2221 cccctcgtcg acctgcgcat cctcgcgcag aaccgcaact tcctcgtcgg ctgcatgctc
     2281 atcttcatgc tcggcttcgc gatctacatc accatcgcca tgatgccgct cttctatcag
     2341 gaagtcctcg gctacacggc gctcaccgcc ggccttgtcg tcgcgccgcg cgggcttgga
     2401 tccatgctcg gcttgcccct gatgggcttc atcagccaca ggatcgacaa ccgctggctc
     2461 atcaccatcg gattcaccgg cttcggcctc ctctccatct ggttctcgct cattaacacc
     2521 ggcatcggcc ctaccacgat gctgatccct atcgtgctca ccggatttgc cctcagcttc
     2581 gtcttcgtcc ccatcggcaa catggccacc gtcaccctca gcaacgaaca aatgggcaac
     2641 gccaccggca tcttcaatct gctgcgcaat atcggtggct ccatcggcat ctcgctcgca
     2701 tcgaccatgc tcatccgccg catggctttc caccaaagcc acatctccgg cactctgccg
     2761 ctcacgggtg tctggtttca gcagcacatg gccgccatgg cggcgtactt cgcgcaccag
     2821 ctcggccccg ctcagggtca acccgccgcc ctggccgcga tgtacatgca gctggagcgc
     2881 caggcgctgc tctggggctt tgtcgatgtc ttccgctgga ccgctctggt ctgcttcgcc
     2941 gcaggtctgg ccgtgtggtc cttccgccgc attagccacg gcgacgacgg cagcgtgaaa
     3001 gtgcactgag ccttcacgct taagcaaaaa gcccgcgcag agcgggcttt ccaactcaaa
     3061 tgaagttccg gactacgacc ttcgatgttc ttccagccgc acaatccgct gtccgtgatc
     3121 ggagatcatg cccagcaaat ccttcacatc ccggttccag atgtccatca tctggtcgat
     3181 tcgttgttca acgcggtcga tacgcccatc caggcgatgc agcccactcc gattcagcag
     3241 aatcccgatc agcgccagga tcgaaggtgc cgcaatgaca acccaaagct ggagctgaga
     3301 catggccacg gtttgattct aagccctacc cgtcgggcac tcgctcaaaa gcaaaccccc
     3361 gcaccaaacc tccgtaccgt tacgcccgat cccgcagctc ccgccacaac cccgcaagca
     3421 cctccacccc cagctcctcc gcgcgcgccc ccgcactcac cccacacttc tccatcgccg
     3481 ccgccacccg tcccgcctcg aatcccgcat tgcgcagatt cttccccagc atcttccgct
     3541 tctgcgcgaa gcagctccgc acaaactgca aaaaagcctc cgcctccacc cccagttctc
     3601 caaaccgagg ccccatcgtc atccgcacca ccgtcgagtg cacctgcggc ggaggagaaa
     3661 acgcccccgg cggaagcgtc atcagcttct ccacccgcgc gtacatttgc gctgtcaccg
     3721 tgagcacgcc ataatcccgc gttccgggca cagccgccaa ccgatccgcc acctcgcgct
     3781 gcaccatcac caccgcacgc tccacatcct ccgcataccg aaacagccgc agcagaatct
     3841 ccgac
//
LOCUS       DASZYN010000438         3223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1114237, whole genome shotgun sequence.
ACCESSION   DASZYN010000438 DASZYN010000000
VERSION     DASZYN010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114237"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="VHE33_11265"
     CDS             <1..1207
                     /locus_tag="VHE33_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVT98077.1"
                     /translation="LPIELSIVMPCLNEAETLASCICKAKDYLERSGVAGEIVIGDNG
                     STDGSQAIALRNGARVVSIPLKGYGAALYGASLAARGPYIVMADSDDSYDFSNLDPFL
                     TKLREGYDLVMGNRFKGGIRAGAMPWKNRYIGNPILSAIGRVFFHCPADDFHCGIRGY
                     SRDAFLRMDLHTTGMEFASEMVIKATMMRMKICEVPTTLSPDGRSRPPHLRPYRDGWR
                     HLRFMLLFSPNWLFLYPGLILVLIGLAGTVFLSFGQIHIRHLRFSIDTLIYCAFMIIT
                     GFQSVLFAVLSRVYAIQERLYPPGRLYNRLFRHIDLERGLIAGILLVLGGLGAAAYAV
                     LEWRRHSFGPLNIEHISRIVIPSGLSITLGMEAILFSFFLSTLGMNVRQHSSVPAAEY
                     ADSEVQAIS"
     gene            1320..>3223
                     /gene="ppk1"
                     /locus_tag="VHE33_11270"
     CDS             1320..>3223
                     /gene="ppk1"
                     /locus_tag="VHE33_11270"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003918.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HVT98078.1"
                     /translation="MRKPSQRAQSLFFSRDESWLGFNRRVLEEAQDSSNPLLERVKFL
                     AITASNLDEFFEIRIAGVLQRIEDGYNEALPDGATLRESLDAIKAETQRFVADQYRCW
                     NDQLLPALRAERIRVLSWEEMDEDAHQFATDYYQREVDPLLTPITIDPSHPFPRVLNK
                     AMCLALLLRRKRRGTAGPVLGVLTVPRALPRFVRIPTADTSEHYVFLADLIERNAGGM
                     YRGYEILSRAAFRVTRNSNLYFQEEEARSLLETVRAELHNRRKGDAVRLEIDSQADPE
                     IVDRLRTNFELDESLVYHSDGPVNLSRIMTLYGSSSRADLKFQPFVPHELRLNRKSVD
                     LFDELRHRDILLHHPYDSYDGVVGFIEAGARDPNVVSMKQTLYRTSADSPIFQALTEA
                     AQTKEVTAVVELMARFDEASNIRWARELEDSGVQVFHGIVGLKTHCKLALQVRRDPDG
                     IVRRYAHLGTGNYNPDTARFYTDLSLLTADPEITAAVHSVFNYLTAHSESDDYRPLLV
                     APLTLAESFIRLIHREAEHAAAGKPARIIAKMNSLLEQSIVEALYAASHAGVQVDLIV
                     RGICSLRPGIKGLSENIRVRSIVGRFLEHSRLFYFFNDGDEEYYCGSADWMPRNLFER
                     CEVIYPIRDP"
BASE COUNT          588 a         1116 c          842 g          677 t
ORIGIN      
        1 attgcccatc gaactcagca tcgtcatgcc ctgtctcaac gaagctgaga ctctggcttc
       61 ctgcatttgc aaggcgaaag actatctgga gaggtcgggc gttgccggcg agatcgttat
      121 cggcgataac ggatccacgg acggttccca ggccatcgct ctccgcaacg gcgcccgggt
      181 cgtcagcatt ccgctcaaag gctacggtgc cgcgctttac ggcgcctcgc tggctgctcg
      241 aggtccgtac atcgtcatgg ccgactccga tgacagctat gacttctcca atctggatcc
      301 atttctgacc aaattgcgcg agggctacga ccttgtgatg ggaaaccgct tcaaaggcgg
      361 catccgggcg ggggccatgc cgtggaagaa ccggtacatc ggaaacccta tcctcagcgc
      421 gatcggcagg gttttcttcc actgtccggc agacgatttt cattgcggca ttcgcggcta
      481 ttccagggat gcgttcctgc gcatggatct ccacacgacg gggatggagt ttgcctccga
      541 aatggtgatc aaagccacca tgatgcgcat gaagatctgc gaagtgccga ccactctcag
      601 ccccgatggc cgctcccgcc cgccgcatct tcgcccctat cgcgatggat ggcggcacct
      661 gcgcttcatg cttctgttca gtccgaactg gctgttcctg tatccggggc tgatcctcgt
      721 gctcatcgga ctggctggaa ccgtgttcct ctcgttcggc cagatccata ttcgtcacct
      781 gcgcttcagt atcgacaccc tgatttactg cgcgttcatg atcattacgg gcttccagtc
      841 ggtgctgttt gcggtgcttt cgcgcgtcta cgcgattcag gagcggctgt acccgcccgg
      901 acgcctttac aaccggctct ttcgacacat tgacctcgaa cgcggcctca tcgcgggcat
      961 tcttctggtt ctgggcggcc tcggggcggc cgcgtatgcg gtgctggagt ggcgacgtca
     1021 cagctttgga ccattgaata tcgagcacat ctcgaggatt gtgatcccct ccggtttatc
     1081 gatcacgctt ggcatggagg cgatcctgtt cagcttcttc ctcagcacgc tcggtatgaa
     1141 cgtccggcaa cactccagcg tgcccgccgc tgagtatgcc gattctgaag ttcaggccat
     1201 ctcctgatcc cttcgaatcc cgagtttttc gcgctaaatt catcatccgt ccgtcgcagt
     1261 accatagaac cgatccgtta aactgcatcg aggcgagctt cggctgccgg ctgaatgtta
     1321 tgcggaaacc atcccagcgc gcgcaaagtc tcttcttcag ccgcgacgaa tcatggcttg
     1381 gcttcaatcg ccgtgtgctg gaagaggctc aggactcgtc caaccctctc ctcgagcgcg
     1441 tcaaattcct cgccatcacc gccagcaacc tcgacgagtt cttcgaaatt cgcatcgccg
     1501 gtgtcctgca gcgcattgag gatggctaca acgaagcttt gcccgacggc gccaccctgc
     1561 gcgagtcact cgacgccatc aaggcggaaa cccagcgctt cgtcgccgac cagtaccgct
     1621 gctggaacga tcagctgctg cctgcgctcc gtgccgagcg catccgcgtg ctctcctggg
     1681 aggagatgga tgaggacgcc catcagttcg ctacggacta ctaccagcga gaagtcgatc
     1741 cgctgctcac ccccatcacc atcgatcctt cccacccatt tccgcgcgtt ctgaataagg
     1801 ccatgtgcct ggcgctgctg ctgcgccgca agcgccgcgg cacggccggc ccggtgcttg
     1861 gcgtcctcac ggttccccgc gcgctgccgc gtttcgtccg catcccaacc gccgatacgt
     1921 ccgaacacta cgtctttctc gccgacctta tcgagcgcaa tgccggcggc atgtaccgcg
     1981 gttacgaaat cctttcccgc gccgccttcc gcgtcacccg caacagcaat ctttacttcc
     2041 aggaagaaga agcgcgctcg ctgctcgaaa ccgtccgcgc ggaactccac aatcggcgca
     2101 aaggcgatgc cgtccgcctc gaaatcgaca gccaggccga cccggaaatc gtcgatcgcc
     2161 tccgcaccaa ctttgagctc gacgagtctc tcgtctacca cagcgacggt ccggtgaatc
     2221 tctcgcgcat catgaccctc tacggctcat cctcgcgcgc cgacctcaag tttcagccct
     2281 tcgttccgca tgagctgcgc ctcaaccgca aatccgtcga tctgttcgac gagctccgcc
     2341 accgcgacat tctcctgcac catccctacg actcttacga cggcgtggtc ggcttcattg
     2401 aagccggagc gcgcgatccc aacgtcgtct cgatgaagca gacgctctac cgcaccagcg
     2461 ccgactcgcc catttttcag gcgctcaccg aagccgcgca aaccaaggaa gtcaccgccg
     2521 tcgtcgaact gatggcgcgt tttgacgagg cctccaacat tcgctgggcg cgcgaactgg
     2581 aagattccgg cgtccaggtt tttcatggca tcgtcggcct caagacccat tgcaagctcg
     2641 ctcttcaggt ccgccgcgac cctgatggca tcgttcgccg ctatgcccac ctcggcaccg
     2701 gcaactacaa tcccgacact gcgcgcttct acaccgatct cagcctgctc accgccgacc
     2761 ccgagatcac cgctgcggtc cacagcgtct tcaactacct caccgcgcac tccgagtcgg
     2821 acgactatcg cccgctcctt gttgcgccgc tcacgctggc cgagagcttc attcgcctca
     2881 tccatcgcga agccgaacat gctgctgccg gcaaacccgc acgcatcatc gcgaagatga
     2941 actctctcct cgagcagagc atcgtcgagg ccctgtacgc cgcttcgcac gcaggagttc
     3001 aggtcgatct catcgtgcgc ggtatttgct cgctccggcc cggcatcaag ggcctcagcg
     3061 agaacatccg ggtccgctcc atcgttggcc gcttcctaga acacagccgc ctcttctact
     3121 tcttcaacga cggcgacgag gaatactact gcggcagcgc tgactggatg ccgcgcaacc
     3181 tcttcgagcg ctgcgaagtt atctacccca tccgcgatcc gca
//
LOCUS       DASZYN010000439         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_399396, whole genome shotgun sequence.
ACCESSION   DASZYN010000439 DASZYN010000000
VERSION     DASZYN010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399396"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="VHE33_11275"
     CDS             complement(<1..1177)
                     /locus_tag="VHE33_11275"
                     /inference="COORDINATES: protein motif:HMM:NF025174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HVT98079.1"
                     /translation="MNKCVFNPCLVLSILAGAALLSSSMAAQESSERLVLEREGRVIS
                     LVPYAPNIVRVTMSSDTKVATSDPGYGITATPSAEGWMHERDADGDDVFRSSQMVVRI
                     GPGNLPEDKLPAPMPLDPLNRELREQYFPGSGGFGPHQDAILITAADGRMLLHMRSWM
                     MTPEAADVAAAHPEDKGHRVMAVFDSPSDEHYYGLGQQQKGWMDLRDRQVRCWHDYGD
                     IGGENVCVPFMVSTRGYGLIWDNPSKTTVDLGFNGRNVWSSEVGDRVSYFVIAGKTTD
                     EIYGGYRLLSGVTHLLPRAVYGYIQSKAIYPTQEQILDLAKGYRDRKLPLDVVVVDFL
                     NMTKQGEMDLDPKRWPDPAAMNRDLHAENVRTLLSVWPHFAPGTQFYDMLESKGWLIH
                     "
     gene            complement(1370..>1606)
                     /locus_tag="VHE33_11280"
     CDS             complement(1370..>1606)
                     /locus_tag="VHE33_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98080.1"
                     /translation="NTYDSPGYKDFDLSFEKYFTFPWFFGEKMKVEGKGEVINLFNRS
                     NLGGVDGSMTNSTFGKSTSQLPARSMQFHLRASF"
BASE COUNT          330 a          465 c          463 g          348 t
ORIGIN      
        1 cgtggatcag ccagcctttg ctttccagca tgtcgtagaa ctgggttccg ggagcaaagt
       61 gcggccagac gctgagcaga gtcctgacat tttccgcgtg cagatcgcga ttcatggcgg
      121 caggatccgg ccagcgcttc gggtcgaggt ccatttcacc ttgtttcgtc atgttcagga
      181 aatccacaac aacgacgtcc agcggtaatt tcctgtcgcg gtaccctttc gccagatcga
      241 ggatctgttc ctgcgttgga taaatcgcct tgctctggat gtatccgtat accgctcgcg
      301 gcaggaggtg ggtaactccg gacagcagcc ggtagccccc gtagatttca tcagtggtct
      361 ttccggcaat gacaaaatac gatacgcggt caccgacctc ggaagaccat acattgcggc
      421 cgttaaaccc gagatccaca gtggtcttcg atgggttgtc ccagatcaag ccgtagccgc
      481 gggttgagac catgaagggg acgcagacat tctcaccgcc aatatccccg tagtcgtgcc
      541 agcagcggac ctggcgatcg cgaagatcca tccagccctt ctgttgttgt ccgagtccgt
      601 aataatgctc gtcggagggg gaatcgaaga ccgccatcac gcgatgtccc ttgtcctcag
      661 ggtgagcggc ggcaacgtcg gcagcttcgg gcgtcatcat ccagctgcgc atatgcagga
      721 gcatcctccc gtcggcggcc gtgatcagga ttgcgtcttg atggggaccg aatccaccgc
      781 ttccgggaaa gtactgctct cgcaattcac ggttgagagg atcgagaggc atgggtgcag
      841 gcagcttatc ctccggcaga ttgcctgggc ctatccgcac aaccatttgc gacgaccgaa
      901 agacgtcatc tccatcggcg tcccgctcat gcatccatcc ctcagccgaa ggcgtcgcgg
      961 tgatcccgta cccagggtcc gaggtcgcga ccttcgtatc gctgctcatc gtcacccgca
     1021 caatgttggg agcatacggc acgagcgaga tcactcgtcc ttcgcgctcg agcaccaggc
     1081 gctcggagct ctcctgcgcg gccatcgaag acgaaagcag cgcggcacca gcaagaatcg
     1141 aaagaacaag acagggattg aaaacgcatt tgttcatgcc acgacctttg ctgtaagttt
     1201 tcacttttgg ttggctgcct tgttagtggt cgccgtgccc acggagcttt atcctggcag
     1261 agggggggta tctccggcgc ggggccgccc taacaggacg atagcgccgg cacgtggcag
     1321 tgaaaagagg ggggagacag cttcatctcc cctcacggag gcacttgggt cagaagctcg
     1381 cgcgcaggtg gaattgcatg ctgcgagcag gtaactggct ggtcgatttt ccgaacgtgc
     1441 tgttcgtcat gctgccatcc actccaccga gattcgaccg gttgaacagg ttgatcactt
     1501 caccttttcc ctcgactttc atcttttcgc cgaagaacca tgggaaggtg aagtatttct
     1561 cgaacgacaa gtcgaagtcc ttgtacccag gagagtcgta ggtgtt
//
LOCUS       DASZYN010000440        13161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_21871, whole genome shotgun sequence.
ACCESSION   DASZYN010000440 DASZYN010000000
VERSION     DASZYN010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 13161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13161
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21871"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..95)
                     /locus_tag="VHE33_11285"
     tRNA            complement(20..95)
                     /locus_tag="VHE33_11285"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(60..62),aa:Ala,seq:ggc)
     gene            242..868
                     /locus_tag="VHE33_11290"
     CDS             242..868
                     /locus_tag="VHE33_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98081.1"
                     /translation="MFFPRVSLALLAAALPLSIFAQGQAPAADPPISVGGTLRPALAQ
                     VSQTLNSISIKHWKAPGDVKSAVDQDVASIRRDLDGTLANLVQQADAAPGSIPAAFAV
                     YRNVDALYDTLLRVVLTANLSASDTETNALQAALSTLESARSQIGESILNASQTQQSE
                     LIRIRTALAHAATVERAPVKTTVVDDGPAETAKHKHSSKKPSSKPNPQ"
     gene            1042..1731
                     /locus_tag="VHE33_11295"
     CDS             1042..1731
                     /locus_tag="VHE33_11295"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVT98082.1"
                     /translation="MQAGTAVGNLAGVIGVRPEEAAVVEQLKAGSEEAFAWLISRYQQ
                     PLYSLIYRVLPNPGDAADITQEVFVKVFRGIGHFHGEASLRTWIYRIALHEALNQRRW
                     WSRHQRQQVTIEPENDDQYSSAGEVLVDPHESPFDAAAHAEVAARVEAALREVPEPFR
                     TVVVLRDIEGFGYEEIAEILNANLGTVKSRLMRGRAHLKNLLAPYMEAARQKSPRSSP
                     APRACSMEEAV"
     gene            1728..2405
                     /locus_tag="VHE33_11300"
     CDS             1728..2405
                     /locus_tag="VHE33_11300"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HVT98083.1"
                     /translation="MNSCRSIRSQFSDYLDGNLTGVAMQSVAGHLRTCGKCSGEFEQW
                     RGVQRSLSRLGPARPPADLALRLRVALSQELAKTPRNALASWKVRWQNTVAPFLLHAS
                     AGLATSIVLVGTMAFLIGAFAAQPASARDEPLGMATGPRFLYSTVEGNAGTVSGKGAP
                     VTVEVYVDRQGRVYDYDIVSGPSDAGTRAELETLLLSSKFEPAQWFGQPVRGVAVLSF
                     AGVSVQG"
     gene            complement(2705..3157)
                     /locus_tag="VHE33_11305"
     CDS             complement(2705..3157)
                     /locus_tag="VHE33_11305"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVT98084.1"
                     /translation="MTPKPLSPRELRIWYAFRMMGEDVLARVDRDIALASGISGPDFA
                     VLSRLAAFGQGQMRQQELGRSLGWDKSRLSHHLTRMQERALIERQPGAATGNGQGVLV
                     VLTQAGREKLDAARPAHADSIRRHLLSRLTADQIDTIIRISNLLGDED"
     gene            3242..4138
                     /locus_tag="VHE33_11310"
     CDS             3242..4138
                     /locus_tag="VHE33_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98085.1"
                     /translation="MSRIRGHRDRQEELHAFLWEAGNEADLLRPDGVAERLRILDALD
                     SVMGDAIGWTAGSAFGIEVHLAARLRKVRRQLEWANEEIFDELRGEIVREGHSRRLWQ
                     MLRGGEGGEEPQGLRFDVRDEIASGVLRLGEPGEPERERSCEMMAYQPTPVRHVLDLI
                     ATSRLAHEDVLVDLGSGLGHVPLLVSILAGIRTLGVELEPAYVACARECAASLGIGRA
                     RFIAEDARAADLASGTVYYLYTPFRGTILEEVLCRLRRESAKRQIRICSLGPCTRELE
                     AEPWLELRAPAEAGRITLFVSR"
     gene            4270..5298
                     /locus_tag="VHE33_11315"
     CDS             4270..5298
                     /locus_tag="VHE33_11315"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVT98086.1"
                     /translation="MVRMTVLASGSKGNSTIISSATTRILVDAGLSCRELMKRMRAAG
                     EEPAALDAILITHEHQDHVQGMAVTARKLGIPVYLTEPTHRAWVRWMTPQKRITYAEW
                     LEQRKASVQRSASSVQEDGDRRSAIGVREDGDQHSTASARCSADIATEPDAEERAAAE
                     EAIAEAEREPKKDPTALPAVEYFRAGSGFRIGDIAVTPFTIPHDAADPVGFVFEADGV
                     RLAIATDLGYMPSNVSVQLRGCDVLMLESNHDVDMLRDGPYPWSVKQRVMSRVGHLSN
                     DAAAEFLAKGYDGGAAYVVLAHLSENNNMPELARISAERALCDRMSLLANHLILAEQD
                     RPLEAIVL"
     gene            5403..5855
                     /locus_tag="VHE33_11320"
     CDS             5403..5855
                     /locus_tag="VHE33_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98087.1"
                     /translation="MSNCTDSVVGDILSSWRYDISGITPEMRVDYEEHLETCAFCHAR
                     QRLHRTIDVVLITLSTVCILAFLLALAVIHHVEPLRSFAVTMHLEHGAVVVTLQAAAI
                     LGLLASLAAWILVAIATPAPGYLTGVAMEQARVLQDRIPEELRSRLRA"
     gene            6061..6279
                     /locus_tag="VHE33_11325"
     CDS             6061..6279
                     /locus_tag="VHE33_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98088.1"
                     /translation="MVISIDAVRYYGKLEATGGGRIQVADGIAEGMKKQAPGVLAPAR
                     GMMFGLLLGGALWVGLVAAVRGVMMMLR"
     gene            6849..6923
                     /locus_tag="VHE33_11330"
     tRNA            6849..6923
                     /locus_tag="VHE33_11330"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6881..6883,aa:Phe,seq:gaa)
     gene            complement(7035..9314)
                     /locus_tag="VHE33_11335"
     CDS             complement(7035..9314)
                     /locus_tag="VHE33_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HVT98089.1"
                     /translation="MSYKRYWVALAIVILGSFAVLGGVGRKMISQAPPIPDVYTTDGQ
                     LLFTGSSITDGQNIWQSIGGQEIGTVWGHGAYVAPDWSADWLHRESLFLLDLWARQAG
                     AASYPAASLDQQAILKARLLRVMRTNTYDPVARRIVIGTDQAEAFRYLSAYYANVFST
                     GRPEYAIPAGALTDRVKLQKMADFFWWTSWAAHTNRPGSSTTYTNNWPHEAPVGNVPP
                     SSAILWSILSFVFLLAGIGALVWWYASREKPDFHPAFPAKDPFLSVTPTPSQRATMKY
                     FFVVVALWGVQILMGVLTAHYGVEGGGFYGIPLDKILPYAVTRTWHLQLAIFWIATSW
                     LATGLYVGPAVSGHEPKGQRLGVNILFGALILVVAGSLAGEWMGIQQKLGNLWFWLGS
                     QGYEYVDLGRLWQILLFVGLVLWLFLMVRSLKPALTRKSESRPLMVLFLISSIAIPLF
                     YAAGLMYGQRSQLVTAEYWRWWVVHLWVEGFFEVFATVVIAFLFTRLNLLEIRSATRA
                     VLFSTTIFLSGGIIGTFHHLYFAGSTPAVIALGAVFSALEVVPLTLVGYEAWENIRLA
                     RGTTNAPWVANYKWPIYFFVAVAFWNMVGAGFFGFLINPPVALYYMQGLNLTPVHGHT
                     ALFGVYGMLGLGLTLFCLRAIKPGLQWKERPLKLAFWTINIGLTFMVVLSMLPIGILQ
                     AIASIEWGTWYARSAEFLQTPSMQFLRWMRVPGDILFATGAAVLGLFVIGLITGHSYE
                     KSGAAARTRTEEGVLVAGD"
     gene            complement(9554..10882)
                     /locus_tag="VHE33_11340"
     CDS             complement(9554..10882)
                     /locus_tag="VHE33_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98090.1"
                     /translation="MSTLPILDDRTALLTWEHRKSLQLRLWIVTGLFFMVLPGTILGF
                     TNLLFISAHHGLAGLSAAWIQAHGHSQVFGWIGSFVLGIGFYSQPRTRANSGLLPPIC
                     WGLWAAGLILHWLAGAWLWLWRITLPLSGAFELVAILLFLHAARQHKVSHSPAAGQSK
                     PRMEAWMQTVLISNFALLLAIVVNFAMGVRLAIEDAGPALAHGFDQRYLVLLGWGFLA
                     PLVWGFSARWLSTFLGVGAVRSRLLQIAVACDVLGVAFGMAGKAVPAALLLAVAAVLA
                     AIALRVFSKSERPAKTIGIHPSFPVFLRLAYAWSIAASLLGIWAALADVHGGIWGGSR
                     HALTVGFAAMMVMTVGPRILPHFAGVRGIRSPGLMFATLLLLAAGCTLRVSMEPLAYE
                     GLGSFAWKLLPYSGCLELGAVLLFALQLVLTFALEPSIFADDRKPAVAAA"
     gene            complement(10981..11682)
                     /locus_tag="VHE33_11345"
     CDS             complement(10981..11682)
                     /locus_tag="VHE33_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HVT98091.1"
                     /translation="MHEPSNVASALGAVSLFSGLSPEELRLLAARAVRRQFAQGELLF
                     SEGDACRGLHIIASGKLRIFKSSPSGREQVLAVEGPGGSVAELPVFDGGPYPASVSAV
                     EDSQILFISRDDLRRFCLEHPEVALKLLAVVGARLRRLVGIIEELSFATVRQRLVAAL
                     VRLAQTEGTRTDRGIEFQLPGTHQDLAHQLGTVRELVSRNLMRLQAESLLQVDARSIV
                     VRDLPGLTAILESNP"
     gene            11772..12491
                     /gene="ric"
                     /locus_tag="VHE33_11350"
     CDS             11772..12491
                     /gene="ric"
                     /locus_tag="VHE33_11350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HVT98092.1"
                     /translation="MTATATTVRDIALEQPASIRVFEKFGIDYCCGGRKPLAEACQER
                     SLDIATVLAAIERATHGTDESTQEWTAASLEALCEHIVRTHHEYVRREIPRLWQLAQK
                     VVARHGDTHPELLQIQQLIRHAGEDLIQHLGKEEQILFPSIVNMERNLANCGPRSIGC
                     FGSVRNPIRVMMAEHDAAGEALAQIRRLSRDFTPPEGACPTYCGYFQALSEFEQDLHR
                     HVHLENNILFPRAIEMDETCI"
     gene            complement(12493..>13161)
                     /locus_tag="VHE33_11355"
     CDS             complement(12493..>13161)
                     /locus_tag="VHE33_11355"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HVT98093.1"
                     /translation="KTVLVLMSAGPLAVTWAQDHLPAILSAWYPGETGGTAIARALFG
                     LDNPGGHLPYTVYAGLDNVPPQNDYAVNDGFTYQYFRGLPLYPFGFGLSYTRFAYSHL
                     QITPAASAVDGEMDVSFELQNTGQRAGADVAQLYTHMEKSRAYQPIRSLRAFQRVELQ
                     PGETKTVHLHIPIQQLAWYDTGRHAFVVEPEVYDLQVGSSSDDIRLRGKITVGSMSAL
                     HHER"
BASE COUNT         2585 a         3676 c         4520 g         2380 t
ORIGIN      
        1 tctgtcgtcc gctgggatct ggtggacctg gtcgggatcg aaccgacgac ctcttccatg
       61 ccatggaagc gcgctcccag ctgcgccaca ggcccacgtg aaggactatc ctattttcat
      121 ggagcaggaa cgattcgtca atgaaatgca ggctccggtt ggtgcacggt gtcgggtgcg
      181 cgccggtggc ttgaagggtc tctggcaacc gcgcggtctg aggatgcatc gtcctggtca
      241 tatgtttttc cctcgcgtgt ccctggcgct gctcgcagcg gcgttgcccc tttcgatttt
      301 tgcccaaggt caggccccgg cggcggatcc gcccatttcc gtcggcggca ccctacggcc
      361 agctctggcg caggtgagtc aaacgctgaa cagcatcagc atcaagcact ggaaggcgcc
      421 gggagacgtg aagtcagccg tcgatcagga cgtggcctcg atccgacgag acctggatgg
      481 aaccctggcc aatctggtcc agcaggcgga tgccgcaccg ggttcgattc ccgcggcgtt
      541 cgccgtgtac cggaacgtcg atgcgctgta tgacacgctg ctgcgcgtgg tgctgacggc
      601 gaacttatcc gcttccgaca cagaaacgaa tgcgctgcag gcggcgctca gcacgcttga
      661 gagcgcgcga tcgcaaatcg gcgaatcgat tttgaatgcc tcgcagacgc aacagtcgga
      721 actgatccgg atccggactg ccctggcaca cgcggcaacg gtcgagcgcg ctccggtcaa
      781 gaccacggtc gtcgacgacg gtcctgcgga gacggcgaag cacaaacaca gctccaaaaa
      841 gcccagttcc aaaccgaacc cgcagtaacc cgcctggaat cagatcaaaa tcccctggga
      901 accttgcgcc tccgttccgt gtctaacgga acagagtgag cgaggtacgg aacccaggcc
      961 cggcccgcat aaccggcgtg ccgggttccg ggacactctt ccgggagcca gcagttcctg
     1021 ctaggctgaa gggagttttt gatgcaggcg ggtacggcag tgggcaatct ggcaggggtc
     1081 atcggagttc gtcccgaaga agctgcggtg gtggagcagc tgaaagcggg ctccgaggaa
     1141 gcgtttgcct ggctgatctc gcggtaccag cagcccctgt acagcctcat ctatcgcgtc
     1201 cttccgaatc ctggcgacgc agcggacatc actcaggaag tcttcgtcaa agtttttcgc
     1261 ggcatcggcc atttccatgg cgaagccagt ctgcggacgt ggatctaccg catcgcgctg
     1321 catgaagcgc tgaaccagcg gcgatggtgg tcgcggcatc agcggcagca ggtgacgatt
     1381 gaaccggaga acgacgatca gtactcgtca gccggggaag tgctggtcga tccacatgaa
     1441 tcgccgttcg acgctgcggc ccacgccgag gttgcggcgc gggtggaggc ggcgctgcgc
     1501 gaggtgccgg agcccttccg aacggtggtg gtattgcgcg acatcgaagg cttcggatac
     1561 gaagagattg cagagattct gaacgcgaac cttgggaccg taaagtcgcg actgatgcgc
     1621 ggccgggccc acctgaagaa cctgttggcg ccttacatgg aagccgctcg tcaaaaatcg
     1681 ccgcggtcgt cgccagcacc acgcgcctgc tccatggagg aggccgtatg aattcgtgca
     1741 gaagcattcg atcgcagttc tccgactacc tggacggaaa tctgaccggg gtggccatgc
     1801 agtcagtcgc cggacacctg cgcacgtgcg gcaagtgctc cggggagttc gagcagtggc
     1861 ggggggtgca gcggtcgctg tccaggctgg gtccggcgcg gccacctgcc gatctggcgc
     1921 tgcggctgcg cgtagcgttg tcgcaggagc tggcgaagac gccgcggaat gcgctggcca
     1981 gctggaaagt acgctggcag aatacggtcg ctcccttcct gctgcatgct tcggcgggac
     2041 tggcaacctc gatcgtgctg gttggcacga tggcattcct gatcggtgcg tttgccgctc
     2101 agccggcatc ggcacgtgac gagcctttgg gcatggcgac cgggccgcgc ttcctctact
     2161 cgacggtgga agggaatgcg ggaacggttt cgggcaaagg cgctccggta acggttgagg
     2221 tgtatgtcga ccggcagggc cgggtctacg actacgacat cgtgtcggga ccgagcgatg
     2281 caggcacccg ggcggaactg gagacgctgc tgctctcgag caagttcgag ccggcgcagt
     2341 ggtttggcca gcctgtgcgc ggggtggcag tgctgtcgtt tgccggcgtg tcggtccagg
     2401 gctaggtccg ctgccagctg aggtccggtt agctaagaac cggtcagcga aaaagctgtc
     2461 agcgaaagac agtcttgcgg gttgggcggt ttggcgcgga catcggatgt tttataaaaa
     2521 caagaagggc tgcctccgtg gcggcccttc tcgtttctga ctccctgcta tcggaatcgc
     2581 aggttgagtg cccgttgaga acgcctcggt gccgagggtg cgtgccgggg aaacggttct
     2641 ggtcgcctga gatcctgtcg aagggggagc tgagtgcgac cgggctgccg gtgcgtcgtg
     2701 ggagttagtc ttcgtcgccg agaaggttgc tgatgcggat gatcgtgtcg atctgatcgg
     2761 ccgtcaggcg cgagagcaga tgacgtcgga tggagtcggc atgggccggg cgcgcggcat
     2821 cgagtttctc ccggcccgcc tgggtgagga caacgagaac gccctgtccg tttcccgtag
     2881 ctgcgcccgg ctgacgctcg atgagggcgc gttcctgcat gcgcgtgagg tggtgggaga
     2941 ggcggctttt gtcccatccc aggctgcggc cgagttcctg ctgacgcatc tggccctggc
     3001 cgaaggcggc gaggcgggag agcacggcaa agtcggggcc ggagattccg ctggccaggg
     3061 cgatgtcgcg gtcgacacgg gcgagcacgt cttcgcccat catgcggaag gcgtaccaaa
     3121 tacgcagctc gcgcggggag agaggtttcg gggtcatgct ggttccgagg ttagcggatt
     3181 gtggttgaca tgacaaccta tattctgtag cgtggttgat atgtcaacca taatcgacag
     3241 cttgtccagg attcggggtc atcgcgaccg gcaggaggaa ctgcatgcgt tcctttggga
     3301 agcggggaat gaagcggatt tgttgcggcc ggatggagtg gcggagcggc tgcggatcct
     3361 cgatgcgctg gattccgtaa tgggcgacgc catcggatgg acggccggtt ccgctttcgg
     3421 gatcgaagtc catttggcgg cacgtctgcg aaaggtccgg aggcagctgg agtgggccaa
     3481 cgaagagatc ttcgatgagt tgcgcgggga gatcgtgcgc gaaggccatt cgcgcaggtt
     3541 gtggcagatg cttcggggtg gggaaggcgg ggaggaaccg cagggtctgc gcttcgatgt
     3601 tcgtgacgag atcgcgagcg gcgtgctgcg gctgggcgag cctggcgagc ccgaacggga
     3661 gcgttcgtgc gaaatgatgg cgtatcagcc cacgccggta cggcatgttc tggacctgat
     3721 cgcgacaagc agacttgctc acgaagatgt cctggtcgat ctgggatcgg gattgggcca
     3781 tgtgccgctg ctggtctcga tcctggccgg gattcgaacg ctgggcgtgg aactcgagcc
     3841 ggcctacgtc gcctgtgcgc gggaatgcgc tgcgagcctg ggaatcgggc gggcgcggtt
     3901 catcgcggaa gatgcgcgcg cggccgatct ggcgagcgga acggtgtact acctgtacac
     3961 gccgttcaga gggacgattc tggaggaggt tttgtgcagg ctgcgcaggg aaagcgcgaa
     4021 gaggcagatt cggatttgtt cccttgggcc gtgcacccgt gaactggagg cggagccgtg
     4081 gctggagctg agggcgccag cagaggccgg gcggattacg ctctttgtct ctcgatgatc
     4141 ggttgaggtc gattcaaaac ggtttccgga ccgaaatggg catgcgccac aatctgtggt
     4201 acccaggtgg agtcccggcc cgaggattgg taccgacatt ccacatggat caggggtatc
     4261 ctgcgagcta tggtgcggat gacggtgctg gccagcgggt cgaaggggaa cagcacgatt
     4321 atctccagcg cgacgacgcg catcctggtg gatgcgggac tgagctgccg ggagctgatg
     4381 aagcggatgc gggcggcggg ggaagagccg gcagcgctcg acgcgatcct gattacgcat
     4441 gagcaccagg accatgtgca ggggatggcg gtgacggcac gcaagctggg gatcccggtg
     4501 tatctgacgg agccgacgca ccgggcgtgg gtgcggtgga tgacaccgca gaagcgaatc
     4561 acctacgcgg agtggcttga gcagcgaaaa gccagcgttc agcgttcagc gagcagcgtt
     4621 caggaagatg gcgatcggcg ttccgcgatc ggcgttcggg aagacggcga tcagcattca
     4681 accgccagcg cgcggtgttc ggcggatata gcgaccgagc cggatgcgga ggagcgagcg
     4741 gctgctgagg aagcgattgc cgaggccgaa agggagccga agaaggatcc gacagcgttg
     4801 ccggctgtgg agtatttccg cgcagggagc ggatttcgga tcggggacat tgcggtgacg
     4861 ccgtttacca tcccgcacga tgcggctgat cctgttgggt ttgtctttga ggctgacggg
     4921 gtacgtctgg ccattgcgac cgacctgggg tatatgccat ctaacgtgtc agtgcagctg
     4981 cgaggctgcg acgtgctgat gctggagtcg aaccacgacg tcgatatgct gcgggatggg
     5041 ccgtatccct ggtcggtgaa gcagcgagtg atgtcgcggg tagggcactt atccaacgat
     5101 gcggcagcag agtttctggc gaagggctac gacgggggcg cggcgtatgt ggtgctggcg
     5161 catttgtcgg agaacaataa catgccggag ctggcgcgaa tcagcgcgga acgggccctg
     5221 tgcgaccgga tgagcctgtt ggccaatcac ttgatattgg cagagcagga tcggccgctg
     5281 gaagctattg tcctgtaact ggacaggttt atactggaac agagccgccc cttcgttgga
     5341 ttctcacctc agggctgtcg gccgggcgtt gacggggtga ggggaaaatg aggacctgaa
     5401 cgatgagtaa ctgcaccgat tcggtcgttg gggacatatt gtcgagctgg cgttacgaca
     5461 tttcggggat cacccccgag atgcgcgtgg attacgagga acatctggag acgtgtgcgt
     5521 tctgccatgc gcggcagagg ctgcaccgga ccattgatgt agttcttatc acgctaagca
     5581 cggtctgcat tttggcgttc ctgctggctc tggcggtgat ccaccatgtc gagccgctgc
     5641 ggagttttgc ggtcaccatg catctggagc acggggcggt cgtggtgacg ctgcaagccg
     5701 cggcgatcct ggggctgctg gcatcgctgg cggcatggat tctggtggca attgcgacac
     5761 cggcgccggg atatctgacc ggtgtggcga tggagcaggc gcgggtgctg caggaccgta
     5821 tcccggagga gctgcgcagc cggctgcggg cataacgaat ttcgaggttg gaagaggggg
     5881 tcccgggaat ccgggacccc ctttgacttt ggggccggtg tgtcccgaaa ggggacgctt
     5941 tcctgtttcg gggaaactgg accctacgtc tggcggatcg tgattttcat cacatccaca
     6001 tgcagagagg ctttgtcacg atggcagtgt tcctgaggtt aggtggtgga ggggctggcc
     6061 atggtgatct cgatcgatgc ggttcgttat tacgggaagc tggaggcgac gggcggcggg
     6121 cggattcagg ttgccgatgg cattgctgaa ggcatgaaga agcaggcgcc tggggtgctg
     6181 gcgccggcac gggggatgat gttcggcctg ctgttgggcg gcgctctctg ggtagggctg
     6241 gtggcggcgg tgcgcggcgt aatgatgatg ttgcgttagg gcgctcagct cgggttgagg
     6301 tgtggaactg gaagggacgc cggttgattg gcgccccttt ttattgcgat gggctgccag
     6361 attctggtgg ttgggcgatc agcggcgcgc ctcgccagag cgttccggcg agggagtgat
     6421 cgcaggcccc tgctcatctt tagcctcgac aacggagtcg tcggcagtgg gacggccgtt
     6481 gcaatcgctt tttgcgccca gggagttttt gctgccggcg ggcgcggagg gatcgattgg
     6541 ggtcgaacag gtgtcggcag ctgggcgatc agcctgatct atggggcgcg tggaaactgg
     6601 agtcatcgga aatcctccct gggttttgtg acgatcagca agaagttaag atgcgagaat
     6661 cgcgaccgcg cagccggagg catgcgggcg atggcaggtt catgacccga gaatttggtt
     6721 tgttatactt gcaaaggtcg ttcgactccg gcaagccaac acggcgggct ggagcccggt
     6781 aaggcgagcg ctgcaatttc gatcgcctgc tccccggacg gctcctatcc gtgaagggtc
     6841 cctcccgcgg ccaggtagct cagtggtaga gcgcggccct gaaaaggccg gcgtcggcgg
     6901 ttcgatcccg tccctggcca ccactcatca tccaagtacg tcgatccgct gtcttcagcg
     6961 ggaagctgcg gctcttgcaa gcaagagcgg cggatcacga aggatccgcc gcccgaattc
     7021 cggagcttca ggaactagtc gccggctacc agaactcctt cctcggtccg ggtccttgcg
     7081 gctgctccgc tcttttcgta agagtggccg gtgatgaggc cgatgacgaa cagtcccaat
     7141 acagcggcgc ccgtggcgaa gagaatgtcc ccgggcacgc gcatccagcg caggaactgc
     7201 atggagggtg tctgcaggaa ctcagcggag cgggcgtacc acgtccccca ctcgatggag
     7261 gcgatggcct gcagaatccc gatgggcagc atgctgagta cgaccatgaa cgtgaggcca
     7321 atattgatgg tccagaaggc cagcttcaat gggcgctcct tccactgcag cccgggcttg
     7381 atggcgcgga gacagaagag ggtgaggccg aggcccagca tgccgtagac gccgaacaga
     7441 gccgtgtgac catgaaccgg cgtcaggttg agtccctgca tgtaatagag cgcaacgggc
     7501 ggattgatga ggaagccgaa gaatcctgcg cccaccatat tccagaaagc aacggcaacg
     7561 aagaagtaga tcggccattt gtagttggcg acccagggcg cgttggtggt gccgcgcgcc
     7621 aggcgaatgt tctcccacgc ctcgtagccg accagcgtta gcgggacgac ttcgagcgcg
     7681 ctgaataccg cgccgagagc gatgacggcg ggggtcgatc cggcgaagta aagatggtgg
     7741 aaggtgccga tgatgcctcc ggacaggaag atcgtggtgg agaagaggac ggcacgcgtg
     7801 gcggaacgaa tctccagcag gttgaggcgg gtaaagagga aggcaatcac cacggtggcg
     7861 aatacttcga agaagccttc aacccagagg tgaacgaccc accaacgcca gtactctgcg
     7921 gtgacgagct ggctgcgttg gccatacatc agaccggcgg catagaaaag cgggatcgcg
     7981 atggaggaga tcaggaagag gaccatcagc ggacggctct cgctcttgcg agtaagcgcc
     8041 ggtttgagcg agcgcaccat caggaagagc cagaggacca atccgacgaa aagcaggatt
     8101 tgccagaggc ggccgaggtc gacgtattcg taaccctggc tgcccagcca gaaccacagg
     8161 ttgccgagct tttgctggat gcccatccat tcgccggcga gcgatccggc gacgacgagg
     8221 atgagggcgc cgaagaggat attgacgcca aggcgctggc ccttcggctc gtgtccactg
     8281 acagcgggtc cgacatagag gccggtggca agccaggagg tggcgatcca gaaaatggcg
     8341 agttgcagat gccaggtgcg ggtgacggcg tagggcagaa tcttgtcgag cggaatgccg
     8401 tagaagccgc cgccttcaac accgtaatgc gcggtgagga cgcccataag gatttgaacg
     8461 ccccacaacg caacgactac gaagaaatat ttcatcgtcg cgcgctgact gggggtgggg
     8521 gtcacgctaa gaaacgggtc tttggcgggg aatgcgggat ggaagtcggg cttctcgcgc
     8581 gaggcgtacc accagacgag ggcgccaatg ccggcgagaa gaaagacgaa gctgaggatg
     8641 ctccagagaa tggccgagga aggcggcaca ttgccgacgg gcgcttcgtg gggccagtta
     8701 ttggtgtagg tggtggagga gccggggcgg ttggtgtgcg cggcccagga agtccaccag
     8761 aaaaagtcag ccatcttctg caacttcacg cgatcggtga gggctccggc aggaatggcg
     8821 tactcgggac ggccggtgct aaagacgttg gcgtagtaag cggagaggta gcggaatgct
     8881 tctgcctgat ccgtgccgat gacgatccgc cgggccacgg ggtcgtaggt gttggtccgc
     8941 atcacgcgca ggaggcgagc cttgagtatc gcctgctggt cgaggctggc ggcgggatag
     9001 cttgccgcac ctgcctgccg ggcccagaga tcgaggagga agaggctctc gcgatggagc
     9061 cagtcggcgg accagtcggg ggcgacgtag gcgccgtggc cccagacggt gccgatctcc
     9121 tggccgccaa tggactgcca aatgttctga ccatcggtga tggaggagcc ggtgaagagc
     9181 aactgtccgt ccgtggtgta gacatcgggg atcggcggag cctgactgat cattttgcgg
     9241 ccgacaccgc caagaacagc aaaggatcca agaatgacga tggccagagc cacccaatag
     9301 cgcttatagg acatgattgc ctctttgcag actgccgatt tctgtggtgc tcatcctgaa
     9361 gccacagtag cggcggaggg atggacaatg cagggactta agtcacaggc cggaacggaa
     9421 tctcatcgag cgatccgctg tggggagccg gggactttgc tcatactcag ggaaaactgc
     9481 ctggtcgaac gtgatgaacg tcacacagcc ggttcctggt acgggagcag ctgcaggatt
     9541 tcggaatgag ggttcaggcg gcggcaacgg ctggtttgcg gtcatcggcg aagatgctcg
     9601 gctccagtgc gaacgtcagg accagctgca gcgcgaacag gagcaccgct cccagttcca
     9661 ggcatccgga atacggcagc agcttccacg cgaaggaccc caggccctcg taggcgagcg
     9721 gctccatgct cacgcgcagc gtgcaacctg cagccagcag caggagggtc gcaaacatca
     9781 ggcccggact tctgatcccg cgcacaccgg cgaagtgggg aagaatgcgc ggtcctaccg
     9841 tcatcaccat catggcggca aatcctaccg tcagggcatg acgcgagcca ccccagatgc
     9901 cgccgtgcac gtcggccagc gcggcccaga tgcccagcag cgatgccgca atcgaccacg
     9961 cataagcgag gcgcaggaat acggggaagc tcggatgaat cccgatcgtc ttcgccgggc
    10021 gctccgattt cgagaagact cgtaatgcga tcgcagccag aacggcggca accgccagaa
    10081 gcagcgcggc cggaacagct tttccggcca tgccgaaggc gacgcccagc acatcgcagg
    10141 caacggcgat ctgcagcaga cgactgcgca ccgctcccac accgaggaat gtcgagagcc
    10201 agcgtgccga gaagccccac accagggggg caagaaatcc ccaacccagc agcaccagat
    10261 agcgctgatc gaatccgtgt gccagtgccg ggcccgcgtc ctcgatggcc agacgcaccc
    10321 ccattgcaaa attgacgacg atcgccaaca acaaggcaaa attgctgatc agcacggttt
    10381 gcatccacgc ctccatgcgc ggcttcgatt gcccggcagc aggagaatgc gagactttgt
    10441 gctgccgcgc cgcatggaga aacagcagga tcgcgacaag ttcgaacgcc ccggaaaggg
    10501 gcagggtgat gcgccagagc cacagccagg cacccgccag ccagtgcagg atcagtcccg
    10561 cggcccacag cccccagcaa atcggcggca gcagcccgct gttggcgcgt gttcgcggct
    10621 gcgaatagaa cccgatgccg aggacaaagc tgccgatcca gccaaagacc tgcgagtggc
    10681 cgtgagcctg aatccatgcg gcggacaacc cagcgagccc gtggtgtgca ctgatgaaga
    10741 gcaggttcgt gaagcccaga atcgtgcccg gaagcaccat gaagaagagc ccggtgacaa
    10801 tccaaaggcg gagctggagg ctcttgcggt gctcccatgt caggagggcg gtgcggtcat
    10861 cgaggatcgg aagtgtggac attgaagttt cctgtcctga gagtggcagg gcaggaagcc
    10921 aggatcagtg acattagtca cgcggtgacc ctaattgccg gtgaatctgg ttagggactt
    10981 ctacggattg ctctcgagga tcgccgtgag tccgggcagg tcgcgcacga caatactgcg
    11041 ggcatccact tgcagcaggc tctccgcctg caggcgcatc agattgcgcg aaaccagttc
    11101 ccgaacggtt cccagctgat gcgccagatc ctggtgggtc cccggcaact ggaattcgat
    11161 cccgcgatcg gtccgtgttc cctctgtctg cgcgagccga accagggccg ccaccagccg
    11221 ctgccgcaca gtcgcaaacg aaagctcttc gatgatgcct accagccgcc gcagccgtgc
    11281 gcccacgacg gccagcagct tcagcgctac ctcgggatgt tccaggcaga agcgccgcag
    11341 gtcgtcccgc gagatgaaca ggatctgcga atcctcaacc gcactgaccg atgcaggata
    11401 gggaccgccg tcgaagaccg gcagttcggc aaccgatccg cccgggccct cgaccgccag
    11461 cacctgttcg cgtccgctcg gtgagctttt gaagatgcgc agcttgccgc tggcgatgat
    11521 atgcaagccc ctgcaggcat caccctcgga gaacagcagt tcgccctggg caaactgcct
    11581 gcgcacggcg cgtgctgcca acaggcggag ttcttccggg gaaagccctg agaagagcga
    11641 gacagcgcca agagcggagg ctacgtttga gggttcgtgc atcggcaaat tctaaccaaa
    11701 atggggattg agtgacttta gtcgcagcct cgcctttgac gcttccgtag agtcggctgc
    11761 aggagatcct catgaccgcc actgcaacga ccgtccgcga catcgctctg gaacaacccg
    11821 catccattcg cgtctttgag aaattcggta ttgactactg ctgcggcggc cgtaagccgc
    11881 tggcggaggc ctgccaggag cgttcgctcg acattgcgac ggtgctggct gccattgaga
    11941 gggccaccca tggcacggat gagagcacac aggaatggac cgccgcttcg ctcgaagcgt
    12001 tgtgcgagca catcgtcaga acgcatcacg agtacgtccg ccgcgagatc ccccgcttgt
    12061 ggcaactggc acaaaaggtc gtggcgcgcc atggcgacac ccatccggaa ctgctccaga
    12121 ttcagcagct gattcgccac gccggcgagg atttgatcca gcacctcggc aaagaggagc
    12181 agattctctt tccatcgatt gtcaatatgg aacgcaatct ggccaactgt gggccgcgct
    12241 cgatcggctg ttttggctca gtgcgcaatc cgatccgcgt gatgatggcc gagcacgacg
    12301 cggctggcga agccctggcc cagattcggc ggctgagccg tgacttcact ccaccggaag
    12361 gcgcctgccc gacgtattgc ggctattttc aggctctttc ggaattcgag caggacctgc
    12421 accggcacgt tcatctggag aacaacatcc tgtttccgcg tgccatcgaa atggacgaga
    12481 cctgtatctg aatcatcttt cgtgatgcaa cgcgctcatg gagccaacgg tgatttttcc
    12541 gcgaaggcgg atgtcgtctg aggaagagcc gacctgcaga tcgtagacct caggctcgac
    12601 gacgaaggcg tgacgtcctg tgtcgtacca cgctagctgt tggatcggta tgtggagatg
    12661 gactgtcttc gtctcaccgg gttgtaactc gacccgctgg aaggcacgca ggctcctgat
    12721 cggctggtaa gcgcggctct tctccatgtg cgtgtagagc tgtgccacgt ctgctcccgc
    12781 gcgttgtccg gtgttctgca gttcgaaaga gacgtccatt tctccatcga cggcagaggc
    12841 agctggagtg atctggagat ggctatacgc gaaccgcgta tagctcaggc caaagccgaa
    12901 cggatacagg ggcagccctc tgaagtactg gtaggtgaat ccgtcgttca cggcatagtc
    12961 gttttgggga ggcacgttgt ccaggccggc atacacggtg taaggaagat ggccgccggg
    13021 gttatcgagt ccaaagagag cccgggcaat ggcggtgccg cctgtctcgc cgggatacca
    13081 ggcgctgagg atcgccggca gatgatcctg cgcccaggtg acggccaacg gccccgcgct
    13141 catgagcacg agaacggttt t
//
LOCUS       DASZYN010000441         2736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_726835, whole genome shotgun sequence.
ACCESSION   DASZYN010000441 DASZYN010000000
VERSION     DASZYN010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726835"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VHE33_11360"
     CDS             complement(<1..398)
                     /locus_tag="VHE33_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT98094.1"
                     /translation="MSRLKNKVAVVTGASKGIGAGIAKGLAAEGAAVVVNYASSNTDA
                     EKVVAEIKSKGGKAVAVQGDVAKAVDVERLFAETKKAFGKVDVLVNNAGVYEFAPIGD
                     VSEQHFHRMFDTNVLGPILATREAAKYLGEG"
     gene            complement(1544..1837)
                     /locus_tag="VHE33_11365"
     CDS             complement(1544..1837)
                     /locus_tag="VHE33_11365"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVT98095.1"
                     /translation="MAGTRKIRLTARQISRIARALADPRRLQILEQIGKRGGSAACTA
                     IRECQPVTAATLSHHMKELETAGLIRISREGKFATLELDREVWQAYLDHLAKI"
     gene            1994..>2736
                     /locus_tag="VHE33_11370"
     CDS             1994..>2736
                     /locus_tag="VHE33_11370"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT98096.1"
                     /translation="MPNFDQHPAGNFVWFELSTSDQAAAAQFYSSLFGWQAQDSPMGP
                     DMIYTMFRLNGRDVAGAYTMKPEDRELGIPPNWLVYIAVANTDAAIASALEHGGSAMS
                     PAIDIPNVGRMAVLQDPVGAVFAVFEPGHHKGVGIYSEPNAFCWADLETPDRERTVPF
                     YTSLFGWQLLPGKDKPADSYLHIKNGEQYIGGIPESRNLPPSVMPHWLPYIQCTDCAA
                     KTARAEQLGARVIVPTTTVEGQLSYSVVA"
BASE COUNT          593 a          981 c          611 g          551 t
ORIGIN      
        1 ccctcgccca ggtactttgc cgcttcgcgc gtcgccagaa tcggtcccag cacgttggtg
       61 tcgaacatgc ggtggaagtg ctgctccgaa acgtcgccga tcggcgcaaa ttcgtagacg
      121 cctgcattat tcaccagcac atccaccttc ccaaatgcct tcttcgtctc cgcaaacagg
      181 cgctccacat ccacggcttt cgccacgtcg ccctgcaccg ccacggcttt gccgcccttg
      241 ctcttgatct ccgccaccac tttctcggcg tccgtgttgc tgctcgcgta attcaccact
      301 accgcggcgc cttccgctgc cagccccttc gcaatccctg caccaatgcc ctttgaagcg
      361 cccgtcacta ccgcgacctt gttcttcagc ctgctcatcg ctcatcctcc caaatcattt
      421 cgatacttag atacatgtct aagcatagaa tggatgagcc gccgtctcga aagggttcag
      481 catttgttcg aatccggcag gttgccgaag accctgatct gaccgaacac gaattccaaa
      541 ccgaaaatac ccgctcggaa atcgttttgg aacaggctcc agaaaccttc acccactttg
      601 ggaagcggcc aggttcaccc cacagcaagc agaagactcc ccggaccgcc actcgtccag
      661 tgatttcctc accggctaaa accgaggtac ccactcagtg ccgtttggga cccgctccgg
      721 aaaaccttca cccattttca ggaagcggcc aggttcatcc cacagcaagc agaagactct
      781 ccggaccgcc actcgtccag tgatttcctc accgactcaa accgaaagta cccacccgga
      841 gccgttttga aacacgctcc agaaaccttc acgcaatttc gggaagcggc caggttcacc
      901 ccacagcaag cagaagactc tccagcgagc cactcgtcca gtgattttct caccgactca
      961 aaccgaaagt acccgcttga aaccgttttg gaacacgctc cagaagacct tcacgcaatt
     1021 tcgggaatcg gccaggttca ccccacagca agcagaagac tctccagcga gccactcgtc
     1081 cagtgatttc ctcaccgact caaaccgaaa gtacccgttc agtgccgttt tggaacgcgc
     1141 tccagaaaac cttcacccat tttcatgaag cgttttttcg gaaagagctg aggagtcgac
     1201 acatgacacg gtgccgggca gacgcgacat catatgcgca atcatgacct caagaagagc
     1261 agtttcagcc gctgtggccc gccaatccgt gcgaagtctc atgagccgtc cgatccgaaa
     1321 acatactgta gcaacaccgt cggatccgaa aacaagttcg cgcgacgttc atgtcgcgca
     1381 tccgtgaccg tcctcaaaaa gatctgcttc agtcgctggc ccccccacca actcacgacg
     1441 aacccttaac cacagaatct ccccgacagc cttcgatgtc acaggcagcg aagaacccca
     1501 aaaagagcag cccggctgag actgttgctc tttcagcagc ctgtcagatc ttcgccaaat
     1561 gatccagata cgcctgccac acctcgcgat ccagctccag cgtcgcgaat ttcccctcac
     1621 gcgaaatccg aatcagcccc gccgtctcca gttccttcat atggtgcgac agcgttgccg
     1681 ccgtcaccgg ctggcattcg cggatcgccg tacacgccgc cgatcccccg cgcttcccaa
     1741 tctgttccag aatctggagc cgccgcggat ccgccaacgc tcgcgcaatc cgcgaaatct
     1801 gccgcgccgt cagccggatt ttcctggttc ctgccatcct cgcccttaag agtaaccctt
     1861 cccgaatttt cgctctttgc tgaccggttt cttgctgact ggcttttagc taagcgtttc
     1921 actgccaacc gtttttaagc tcaagaaaga aatttcatcc ccatgcctct ccattccgcc
     1981 tacaatctcc gccatgccca acttcgatca gcatcccgcc ggcaatttcg tctggttcga
     2041 actctccacc tccgaccagg ccgccgccgc gcagttctac tccagcctgt tcggatggca
     2101 ggcgcaggac tctcccatgg gcccggacat gatttacacc atgttccgct taaacgggcg
     2161 cgacgttgcc ggtgcataca ccatgaagcc cgaagatcgt gagctcggca tcccaccgaa
     2221 ctggctcgtc tatattgccg tcgccaacac cgacgccgcc atcgccagcg ccctcgagca
     2281 cggcggcagc gccatgtccc ccgccatcga cattcccaac gtcggccgca tggccgttct
     2341 ccaggatccc gtcggcgcgg tgttcgctgt cttcgagccc ggccaccaca aaggcgtcgg
     2401 catctacagt gaaccgaatg ctttctgctg ggccgacctc gaaactcccg atcgtgaacg
     2461 aaccgtgccc ttctacacct ccctcttcgg atggcagctg cttcccggca aagacaaacc
     2521 tgccgacagc tacctgcaca tcaaaaatgg cgagcagtac atcggcggca ttcccgaatc
     2581 ccgcaatctg ccgcccagcg tcatgcctca ctggcttccc tacatccagt gcaccgactg
     2641 cgctgcgaaa accgcaaggg ccgaacaact cggcgctcgc gtgatcgttc caaccaccac
     2701 cgtcgaaggc cagctcagct actccgtcgt cgccga
//
LOCUS       DASZYN010000442         5428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_470324, whole genome shotgun sequence.
ACCESSION   DASZYN010000442 DASZYN010000000
VERSION     DASZYN010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470324"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1636
                     /locus_tag="VHE33_11375"
     CDS             167..1636
                     /locus_tag="VHE33_11375"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HVT98097.1"
                     /translation="MKGLDQAKDGRLPEPIATTESAQGKPHLERRIGLANAVTLNMMY
                     MIGVGPFITLPLIVAAMGGSQALLGWLLGAAIAVCDGLVWAELGAAMPEAGGSYTFLR
                     EIYGREGAGRFVSFLYIFQLGFSAPLSIASGCIGFAEFGSFMLPGLRSPLFAAWPHGP
                     HWTNVAAAGACAVIVGMLYRDIRAILRTAWVLWAGVMAAMAVVLIAGFTHFHPALLRM
                     SPHALSFSPAFFAGLGSATLIATYDYWGYYNVCFLGGEIREPGRTIPRAVMLSILVVA
                     VLYIAMNVSVLGVIPAQTMHQVAQTVVAEMVARTLGPAWAVAIACLIMWTAFSSVFAL
                     LMGYSRVPYAAALDGNYFRIFAHLHPKRKFPDFSLIALGAVAAVFCFFRLAQVIAALV
                     VIRIVLQYLLQQVGVMVLRAKQPDLARPFRIWLYPLPPILALTGFLFILFSRPQALRY
                     LVWALAIGVVGSVLYLLRARSRGEWPFKTGSDAAHGIAR"
     gene            1633..2319
                     /locus_tag="VHE33_11380"
     CDS             1633..2319
                     /locus_tag="VHE33_11380"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HVT98098.1"
                     /translation="MNGFVLAGGQSTRMGRDKALLEVNGRTLVEHMLEKLRGLGLEAR
                     ICGSRSDLAPFAEVVPDNFAQSGPLGGMEAALAVSDSELNLFVAVDLPGIPEEFLGWL
                     MGRAERSRAVATIPRYGDRLQPLCAVYSRRLVEGLRESLRAGRRKVMDGIRTSALGVG
                     ESIDVFDAECVAAAGCTEWLLEPPLAGWFRNVNTPAEYEAAIRVLRNGRPAVQKAVSR
                     VGHPEGPEAR"
     gene            2389..3201
                     /locus_tag="VHE33_11385"
     CDS             2389..3201
                     /locus_tag="VHE33_11385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVT98099.1"
                     /translation="MSFPPDVPIEVKGESNGHGDRPPYIAFENVSKSFGSLDVLRDIS
                     FFVLPGETLCILGRSGVGKSVSLQTIMGFLKPDAGRILVAGEDITDFTEQQMQTVRRK
                     VTMVFQNGALFDSLTVGENVAFPLREGRGLSEDQIQQIVRGLLEMVGVAEMIDLLPSD
                     LSTGMKRSVAIARALAAEPEAILYDEPTTMVDPLIARLLGDLIQKLKKQLHLTSIVVT
                     HDMRFAERLADRVLFLHQGRAQFFGTIEEMKNTKDEILQQFLELDALVLPQV"
     gene            3260..4906
                     /locus_tag="VHE33_11390"
     CDS             3260..4906
                     /locus_tag="VHE33_11390"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HVT98100.1"
                     /translation="MKIALAQIDPTVGDFSGNTAKIIEFTHRAQQGGADLVLFPELAI
                     CGYPPADLLEKPAFVARCGEAICEVARAAAKDGIGVIAGYVTAAEHGAGKHVMNSAAL
                     VRHGHVEFVQSKMLLPYYDVFDEQRYFAPAARQRVCHFDGKPLALTICEDAWNDKSFW
                     ENRLYSVDPVEEQMKQGASLIFNIAASPWWHDKRALRQEMLAAIAKRQRAPVVMVNQV
                     GGNDSLVFDGSSLVIDPSGKVIAQAKSFEEDLIFADLEAMTGDVREQTSSEDEAMLEA
                     LTLGTRDYVRKCGFSKVVVGLSGGIDSALVAAIAVRALGAENVTTLGMPSPYSSQGSI
                     DDSRQLAKNLGIRYEVIAIDEIFSGFNHALEPMFAHSKADITEENLQSRIRGMLLMAF
                     SNKFNALVLTTGNKSEMSTGYCTLYGDMVGALAVIGDVFKTRVYDLCRWINREREVIP
                     RSILEKPPSAELRPGQKDTDSLPPYEVLDPILRAYVERYETPEKIATANGVDVEMVRT
                     VVKMVERSEYKRQQAAPVLKVTPKAFGMGRRFPIAAKAQV"
     gene            4929..>5428
                     /locus_tag="VHE33_11395"
     CDS             4929..>5428
                     /locus_tag="VHE33_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98101.1"
                     /translation="MQRFIYGLMALLVSSVVGFAQATPPSAPTPQQAPTAPAATPGEP
                     QFPPVSEANFNATSPTKADVEAFLKASWGYDENRVWEVYGIEKTTAPGVSKVTILVAE
                     KQTPQQIANLTFFVTPDGKHLIAQESVLDFGAKPYENNYRLLQQRAEGPSRGATAKQF
                     ELVEFG"
BASE COUNT          980 a         1343 c         1991 g         1114 t
ORIGIN      
        1 agggtgagcg cgacggagct gcgaacgatt tggagaagcg gcagagtctt cggaaccatc
       61 gaattgccag tgtaatcgcg atgcgatttg atacaaaggc agagcatgca ggagggtaca
      121 gggtgcggcg tacagggtgc aggttgccgg gcttcgggat gacagggtga agggattgga
      181 tcaagcgaag gacggacggc ttcctgaacc catcgcgaca acggagagcg cgcaggggaa
      241 gccgcatctg gagcggcgga tcgggctggc gaacgcggtc acgctgaaca tgatgtacat
      301 gatcggcgtg gggcccttca tcacgctgcc cctgattgtg gcagcgatgg gcggctcgca
      361 ggcgctgctg ggctggctgc tgggagcggc gatcgcggtt tgcgacgggc tggtgtgggc
      421 ggagctgggt gcggcgatgc cggaggcggg cgggtcgtat acctttctgc gtgagatcta
      481 cggacgggag ggggcgggcc gcttcgtctc atttctctat atttttcagc ttggattcag
      541 cgcaccgctg tcgattgcat cggggtgcat cgggttcgcg gagtttggct cttttatgct
      601 gccggggctg cgatcaccgc tatttgccgc gtggccgcac gggccgcact ggacgaatgt
      661 ggcggcggca ggcgcatgcg cggtgatcgt ggggatgctg taccgggata ttcgggcgat
      721 tctgcgcacg gcctgggtgt tgtgggcggg cgtgatggcg gcgatggcgg tggtgctgat
      781 tgcggggttc acgcattttc accccgcact gttacgaatg tcgccgcatg cgctgagctt
      841 ctcgccggcg ttctttgcgg ggctggggtc ggcgacgctg attgcgacgt acgactactg
      901 gggctactac aacgtgtgct ttctgggtgg agagattcgc gagccagggc ggacgattcc
      961 gcgagccgtc atgctctcga ttttggttgt tgctgtgctg tacatcgcga tgaacgtgag
     1021 cgtgctggga gtgattccgg cgcagacgat gcatcaggtg gcgcaaaccg tggttgcgga
     1081 gatggtggcg cgaacactgg ggccagcatg ggcagttgcc attgcctgcc tgatcatgtg
     1141 gacggcgttc tcctcggtgt ttgcgctgtt gatgggctat tcgcgcgttc cctatgcagc
     1201 ggcactggac gggaactatt tcaggatctt cgcgcacctg catccgaagc ggaagtttcc
     1261 ggatttctcg ctgatcgcgc tgggagcggt ggcggccgtg ttttgctttt tccggctcgc
     1321 gcaggtgatt gcggcgctgg tggtgatccg gatcgtgctg cagtatttgt tgcagcaggt
     1381 gggcgtgatg gtgctgcggg caaagcagcc ggatttggcg aggccattcc gcatctggct
     1441 ttatccgctg ccgccaattt tggcactcac gggattcttg ttcatcctgt tttcgcgtcc
     1501 gcaggcgctg cggtacctgg tttgggcgct ggcgattggc gtggtgggca gcgtcctgta
     1561 tttgctgcgg gcacgaagcc gtggtgaatg gccgttcaag actgggtcgg acgccgccca
     1621 tgggatagcg cgatgaatgg gttcgtgctg gccggcgggc agagcacgcg catgggacgc
     1681 gataaggctt tgctggaggt gaatgggcgg acgctggtcg agcacatgct ggagaagctg
     1741 cgtgggctgg gactggaggc gcggatttgt gggtcgcgct cagacctggc gccattcgct
     1801 gaggtcgttc cggataattt tgcgcagtcg ggtccgctgg gcgggatgga ggcggcgctg
     1861 gcggtttcgg attcggaact caatctgttt gtggctgtcg atctgccggg gattcctgag
     1921 gagtttttgg gctggctgat ggggcgggcc gagaggagcc gcgcagtggc gacgataccg
     1981 cgctacggcg atcgtctgca gccgctttgc gccgtctaca gtcgacgact ggtggagggg
     2041 ttgcgggagt cgctgagagc ggggcgtcgg aaggtcatgg acgggattcg tacgtcggcg
     2101 ttgggggtag gggagtccat cgacgtattt gacgcagagt gcgtggccgc tgcgggttgt
     2161 actgaatggc tattggagcc gccgctggcg ggatggtttc ggaacgtgaa tacgccagcg
     2221 gagtacgagg ctgcgattcg tgttttgcgc aatggtcgcc cggcagttca aaaagctgtg
     2281 agcagagtgg ggcacccgga gggtccggag gccagatgat tgcggctgga gcaaaaagcc
     2341 gtcatccaat aagctgaatg caggtgcgcg aaagggcttc gaacgagctt gagttttccc
     2401 ccggacgttc cgattgaggt caaaggcgag tccaacggtc atggggaccg gccgccctac
     2461 attgccttcg aaaacgtctc caagtccttc ggcagtctcg atgttctgag ggacatcagc
     2521 tttttcgtcc tgccggggga gacgctgtgc atcctgggcc ggtcgggggt aggcaagtcg
     2581 gtttcgctgc agaccatcat gggattcctg aagccggatg cggggcggat tctggtggcg
     2641 ggtgaggaca tcaccgactt caccgaacag cagatgcaga cggtgcggcg gaaggtgacg
     2701 atggtcttcc agaacggcgc gctgttcgat tcgctgacgg ttggcgagaa cgttgcgttt
     2761 ccgctgcgtg aagggcgcgg gctttcggag gatcagatcc agcagattgt gcgcggcctg
     2821 ctggagatgg tgggcgtggc agagatgatc gacctgttgc cgtcggattt gtcgacgggg
     2881 atgaagcgct cggtggcgat tgcgcgcgcg ctggcggccg agccggaagc gatcctgtac
     2941 gacgagccga cgacgatggt ggatccgctg atcgcgcggc tgctggggga cctgattcag
     3001 aagctgaaga agcagctgca cctgaccagc attgtggtaa cgcatgatat gcgatttgcg
     3061 gagcggctgg cggatcgggt gctctttctg catcaggggc gggcgcaatt cttcgggacg
     3121 atcgaggaga tgaagaacac gaaggacgag attctgcagc agttcctgga actggacgcg
     3181 ctggtgctgc ctcaggtgta agagccagcg ttcagctctg cgttcaggag aaggcttcgg
     3241 cgattgctaa actagacctg tgaagattgc gctggcgcag atcgacccca cggtcggaga
     3301 tttcagcggc aataccgcga aaatcatcga atttacgcac cgggcgcagc agggcggcgc
     3361 cgatctggtg ctgtttccgg aactggccat ctgcgggtat cctccggccg acctgctgga
     3421 aaagcctgcg ttcgttgcgc ggtgcgggga ggcgatctgc gaagtcgcgc gcgcggcggc
     3481 gaaagacggg atcggggtca ttgccggata cgtgacggcg gcggagcatg gtgcagggaa
     3541 gcacgtgatg aactccgcgg cgctggtgcg gcatgggcat gtagagtttg tgcagtccaa
     3601 gatgctgttg ccgtactacg acgtgtttga cgagcagcgc tatttcgctc cggcggcgcg
     3661 acagcgggtg tgccatttcg acgggaagcc gctggcattg acgatttgcg aagacgcgtg
     3721 gaatgacaag agcttttggg agaaccgcct gtattcggtc gatccggtgg aagagcagat
     3781 gaagcagggg gcgtcgctga tcttcaacat tgcggcgtcg ccgtggtggc atgataagcg
     3841 cgcattacgg caggagatgc tggcggcgat tgcgaagcgg cagcgcgcgc cggtggtgat
     3901 ggtgaaccag gtaggcggaa acgatagcct ggtgttcgac ggctcgagcc tggtgatcga
     3961 tcccagcgga aaggtgattg cgcaggcgaa gagcttcgaa gaggacctga ttttcgccga
     4021 tctggaggcg atgaccggcg atgtacgcga gcaaacgagc agcgaagatg aagcgatgct
     4081 ggaggctctg acgctgggca cgcgcgatta cgtgcgcaag tgcggattct cgaaggtggt
     4141 ggtgggattg agcgggggca tcgactcggc gctggtagcg gcgattgcgg tgcgggcatt
     4201 gggagcagaa aacgtgacga cgctggggat gccgagcccc tattcctcgc agggctccat
     4261 cgacgacagc cggcaactgg cgaagaatct gggaattcgc tatgaggtca ttgcgatcga
     4321 tgaaatcttt tccggattca accacgcgct ggagccgatg ttcgctcaca gcaaggcgga
     4381 tattacggaa gagaacctcc agtcccgaat tcgcgggatg ctgctgatgg cgttctcgaa
     4441 caagttcaac gcgctggtgc tgacgacggg caacaagagc gagatgtcca ctgggtactg
     4501 cacactttac ggcgatatgg tgggagcgct ggccgtgatc ggcgatgtgt tcaagacgcg
     4561 cgtgtacgat ttgtgccgct ggattaatcg ggagagggaa gtgatccccc gttcgatcct
     4621 tgagaagccg ccgtcggccg agttgcggcc tggacagaaa gatacggact cgctgccgcc
     4681 gtacgaggtg ctggatccga ttctgcgggc ctatgtggaa cgctatgaga cgccggagaa
     4741 gattgcgaca gccaatggtg tcgatgtcga aatggtacgg acggtcgtca agatggtgga
     4801 gcgcagcgaa tacaagcgac agcaggcggc gccggttttg aaggtgacgc cgaaggcgtt
     4861 tggcatgggc cggcggttcc ccatcgcagc taaagcacaa gtttgaaata gtccccgaaa
     4921 ggaaacgaat gcaacgtttt atttatgggc tgatggcgct tctggttagt tctgttgttg
     4981 gttttgcgca ggccacgcct ccttcagcgc caacacccca gcaggcaccc acggctcccg
     5041 ccgccacccc gggcgaaccg cagtttccgc cggtgagcga ggcgaacttc aatgccacgt
     5101 cgccgacgaa ggccgacgtc gaggcgttcc tgaaggcgtc gtggggctac gacgagaatc
     5161 gcgtgtggga ggtttacggc atcgagaaga cgaccgcgcc tggcgtgagc aaggtgacga
     5221 tcctggtggc tgagaagcag accccgcaac agatcgcgaa cctgacgttc tttgttacgc
     5281 cggatggcaa gcacctgatc gcacaggagt cggttctgga tttcggggcg aagccgtacg
     5341 agaacaatta tcgattgctg cagcagcgcg ccgaggggcc gtcgcggggc gccacggcga
     5401 agcagtttga actggtggag tttgggga
//
LOCUS       DASZYN010000443         1843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_150793, whole genome shotgun sequence.
ACCESSION   DASZYN010000443 DASZYN010000000
VERSION     DASZYN010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150793"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..740)
                     /gene="nusG"
                     /locus_tag="VHE33_11400"
     CDS             complement(114..740)
                     /gene="nusG"
                     /locus_tag="VHE33_11400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HVT98102.1"
                     /translation="MAEETENPTPTPEGEGGEGGEQQPQLAPPANESFRWYIIHAYSG
                     FERKVRESIETRVQAFGLQNRIGRVMIPTEAVTEITNGKKRTVERVFLPGYVLVEMDL
                     DNDLWHVIKNTPRVTGFLGTGDKPVALTEAEVSSILFRSDTSKEKPRLKVKFQKAESV
                     RITEGPFANFNGVVDEVNEDRETLKVMVTIFGRSTPVELDFGKVEKIA"
     gene            complement(766..1026)
                     /gene="secE"
                     /locus_tag="VHE33_11405"
     CDS             complement(766..1026)
                     /gene="secE"
                     /locus_tag="VHE33_11405"
                     /inference="COORDINATES: protein motif:HMM:NF012793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HVT98103.1"
                     /translation="MAKAAISNLEQGIPNRALGFVTRSRDFLRDVRAEMRKVVTPSWK
                     EVQSTTLVVIVSVFIFAGFFWVVDAVFGNAIHWLYSWLGAVQ"
     gene            complement(1109..1184)
                     /locus_tag="VHE33_11410"
     tRNA            complement(1109..1184)
                     /locus_tag="VHE33_11410"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1148..1150),aa:Trp,seq:cca)
     gene            complement(1309..1458)
                     /gene="rpmG"
                     /locus_tag="VHE33_11415"
     CDS             complement(1309..1458)
                     /gene="rpmG"
                     /locus_tag="VHE33_11415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HVT98104.1"
                     /translation="MREIVTLQCGECKERNYSTTKNKKTTTSRLEFKKFCNRCRKHTP
                     HKETK"
     gene            complement(1607..>1843)
                     /locus_tag="VHE33_11420"
     CDS             complement(1607..>1843)
                     /locus_tag="VHE33_11420"
                     /inference="COORDINATES: protein motif:HMM:NF015125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98105.1"
                     /translation="GGRHTPFFKGYRPQFYFRTTDVTGVATLPAGTEMVMPGDNVELE
                     VELITPVAMEKGLRFAIREGGRTVGAGTISEIIA"
BASE COUNT          331 a          563 c          522 g          427 t
ORIGIN      
        1 ctcagctgct ggctaccagc ttttagctgt tagctcaaag ttgctactgc gaaaatcctg
       61 cgccgtgggc agggcatagc cagcagccaa aagctgatag ctaaccggag ctcctaggcg
      121 attttttcca ccttgccaaa atcaagttcg accggagtcg aacggccgaa gatcgtaacc
      181 atcaccttaa gcgtctcgcg atcctcgttc acctcgtcga ccacgccatt gaagttggcg
      241 aacggcccct cggtaatgcg gaccgattcg gccttctgga acttgacctt gaggcgcggc
      301 ttttctttcg acgtgtcgct gcggaacagg atcgaactca cttccgcctc agtcagggcc
      361 acgggcttgt cgccggtgcc gaggaaccct gtaacgcgcg gtgtgttctt gatcacgtgc
      421 cacagatcgt tgtcgagatc catctcgacc agcacgtagc caggcaggaa gacgcgctcc
      481 accgtgcgct tcttgccgtt ggtgatctcg gtgacggctt cggtgggaat catgacgcgg
      541 ccgatgcggt tctggagtcc aaaggcctgg actcgcgtct cgatggattc gcgcaccttg
      601 cgctcaaagc ccgaataggc gtggatgatg taccaccgga agctctcgtt ggccgggggg
      661 gctagctgcg gctgttgctc gccgccttcg ccgccctcgc cttcaggcgt cggggttgga
      721 ttttcagttt cttcggccat taccattcaa tcctcttgcg tgctgctact ggaccgcgcc
      781 cagccagctg taaagccagt gaatcgcatt gccgaacacc gcgtcgacga cccagaagaa
      841 gccggcaaag atgaaaacgg agacgatgac gaccaaagtg gtggactgga cttctttcca
      901 cgagggcgtg acaaccttgc gcatctcagc gcggacgtcg cgaagaaagt cgcggctgcg
      961 cgtgacgaag ccgagtgcgc ggttgggaat gccctgctcg aggttggaga ttgctgcttt
     1021 agccatgact cgcttcttct tttctgctct tccgcgctct gttcggcgac agccccggcc
     1081 gggccgactc ctgaagttat tgttgatctg gcaggggcgg agggattcga acccccaagt
     1141 tcggttttgg agaccgacag tttaaccgtt gagcttacgc ccctgtgaaa cagagcttca
     1201 gcgttcagcg atcagcgttc agaatcctgg aggcgatgaa agggggtcgg gcccctcgga
     1261 atgctcagtc gactctaatc cctaatccct aaactctaaa acctgtgttt acttcgtttc
     1321 cttgtgaggg gtgtgcttgc ggcagcgatt acagaacttc ttgaactcga gacggctggt
     1381 cgtcgtcttt ttgttcttcg tcgtcgagta gttgcgctct ttgcactccc cgcactgcaa
     1441 tgtgacaatt tcccgcatct tcctatccta cctgatttcc ctgcatttcc ctatatctgc
     1501 cccgtttggc aggccccagg ggacccacga tggcctttca ctgaccgctt tcctgctgac
     1561 cgcttcttag ccggccggtt tttagccgac cggctttcag ctgccctcag gcgatgattt
     1621 ccgagatcgt gcctgcgccg acggtgcggc cgccttcgcg aatcgcgaag cgcaggcctt
     1681 tctccatggc caccggcgtg atcagctcca cttccagctc cacgttgtcg cccggcatca
     1741 ccatctcggt gccggccggc agcgtcgcca cgcccgtcac gtcggtcgtg cggaagtaga
     1801 actgcggacg atagcccttg aagaacggcg tgtgacggcc gcc
//
LOCUS       DASZYN010000444         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1176743, whole genome shotgun sequence.
ACCESSION   DASZYN010000444 DASZYN010000000
VERSION     DASZYN010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176743"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VHE33_11425"
     CDS             <1..697
                     /locus_tag="VHE33_11425"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HVT98106.1"
                     /translation="ALKNTFTIGIISTVPQLLIALGVAHMLNYTMRWRTFFRVALILP
                     YATSVAAATLVFNQIYSRQTGLANWIVGLFGVHHIDWLNNKWAAQIAISTIVTWRWVG
                     YNALIYLAGMQAIPGDLYESAVIDGASRWQQFRYVTLPGLRPTILFTVIISTIGATQL
                     FAEPLLYNNGTPNGGGLNQFQTLGLLMYQQGWANRNLGLAATTAWTIFLLIVVLVIVN
                     VFLMRRVTHESKG"
     gene            705..>1577
                     /locus_tag="VHE33_11430"
     CDS             705..>1577
                     /locus_tag="VHE33_11430"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HVT98107.1"
                     /translation="MATMTTSVAAPRRRRSARSGQAGGQQRAGKFTYFLIAIITVLSI
                     FPLYWTLVAGSHTNAQMATQPPPLIPTTDFFHNLSMAMQRQNLWQALLNSTIVATVVA
                     VSNVLFCTLAGFAFAKLRFRGSNILLMLVVGTLMVPLQVGIVPLFMLAAKYHMIGHLQ
                     AAIAPYLVSAFGVFLLRQYLIAALPTELVEAGWVDGASTFRIFRSIVLPIARPAMAVL
                     AMFQFLMAWNDFFWPILVLNNRNPTVQVALSGMGTGYVPQTAVIMAATFFVTLPVLIA
                     FGFLGKQIVGGIMQG"
BASE COUNT          255 a          515 c          475 g          332 t
ORIGIN      
        1 cgcgttgaag aacacgttca cgatcggcat catctcgacg gtgccgcagc tgctcatcgc
       61 gctcggcgtc gcgcacatgc tgaactacac gatgcgatgg cggacgttct tccgggtcgc
      121 gctgatcctg ccgtacgcca cgagcgtcgc cgccgccacg ctggtcttca accagatcta
      181 cagccgtcag accggcctcg ccaactggat cgtcgggctt ttcggcgtcc accacatcga
      241 ctggctcaac aacaagtggg cggcgcagat cgccatctcg accatcgtca cctggcgttg
      301 ggtgggctat aacgcgctca tctacctcgc cggcatgcag gcgattccgg gcgacctgta
      361 cgagtcggcc gtcatcgacg gggcgtctcg ctggcagcag ttccgttacg tgaccttgcc
      421 cggactgcgg ccgacgattc tgttcaccgt catcatctcg acgatcggcg cgacgcaact
      481 gttcgccgag ccgctgcttt acaacaacgg cacgcctaac ggtggtggcc tcaaccagtt
      541 ccagaccctg ggcctgctga tgtaccagca aggctgggcg aaccggaacc tcggactcgc
      601 cgcgacgacc gcgtggacca tcttcttgct gatcgtggtg ctcgtgatcg tcaacgtgtt
      661 cttgatgcga cgcgtcacgc acgagtcgaa gggctgatcc ggagatggca acgatgacca
      721 cctcagtcgc cgcccctcgg cgccgtcgca gcgctcgcag cgggcaggcg ggcggtcagc
      781 agcgggctgg caagttcacc tacttcttga tcgcgatcat cacggtgctc tcgatctttc
      841 cgctgtattg gacgttggtc gcgggctcgc acaccaacgc ccagatggcc acccagccgc
      901 cccccctgat ccccaccacc gacttcttcc acaacctgtc gatggcgatg caacgccaga
      961 acttgtggca ggcgctgctg aacagcacga tcgtggcgac ggtggtcgcg gtcagcaatg
     1021 tgttgttttg cacgctggct ggattcgcct tcgccaagct gcgcttccga ggcagcaaca
     1081 tcttgctgat gctggtcgtg ggcacgttga tggtgccgct gcaggtgggc atcgtcccgc
     1141 tgttcatgct ggccgcgaag taccacatga tcggccacct gcaggcggcg atagcgccgt
     1201 atctggtgtc ggcgttcggc gtcttcctac ttcgtcagta cttgatcgcc gcgctgccca
     1261 cggagctcgt cgaggcgggc tgggtcgacg gcgcctcgac cttccgcatc ttccgctcga
     1321 tcgtgttgcc gatcgcgcgt ccggcgatgg ccgtgttggc gatgttccag ttcctgatgg
     1381 cgtggaatga tttcttctgg ccgattctgg tgctcaacaa tcgcaaccca acggtccagg
     1441 tggcgctcag cggcatgggt accggctacg ttccacagac tgcggtcatc atggctgcga
     1501 cgttcttcgt cacgctgccc gtgctcatcg cgttcggctt tcttggtaag caaatcgttg
     1561 gaggaatcat gcaggga
//
LOCUS       DASZYN010000445         2569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1176887, whole genome shotgun sequence.
ACCESSION   DASZYN010000445 DASZYN010000000
VERSION     DASZYN010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176887"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2027)
                     /locus_tag="VHE33_11435"
     CDS             complement(<1..2027)
                     /locus_tag="VHE33_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HVT98108.1"
                     /translation="MRTLDIQLTVDGDRLRCSAPEGRLTPDLEQRIQLAKAELLRVLR
                     APAPATLTIPRRAASDTVLPLSFAQERFWLLQNLDPQSTAYNITAVLPFSGAVDVEAL
                     RWALEEVVRRREVLRTCFPEDRGLPTQVIADEAAPHLEVWDGVALPESKRQNAIDEQI
                     DEMSRQQLDMQRGPLLRLKLLRASDTEQSLVLTVHHILCDAWGLGILLSELRGFYAQR
                     TGAWPWQPPALPIQYGDYAVWERQQEATGAFRAQLDYWKQKLRDVPPYLELPTDRPHS
                     LSLPFEAKLQPVQLGSETSKLLKGHMAEAGATPFMALLAVFQALLHRFTNQQTIVVGT
                     PVSTRTRPELERLIGCLINTHALRADFPAGMTARQLLQQVRGTVLEALTHSDVPFERV
                     VSELVRSRNLARSPLFQTAFIEQKTPHAGEFRIVSGGTTFDLTMYVWETERGFEGSIE
                     YDGHLFDAGTIACWAGSYATLAAAMARHPDTPIDELALVSEEQEAEWFGKSHGASIPV
                     PEESVDQWIERQAMKRPHAVAVVCGQKVLSYREVNERANRLANRLRTLGVGPETLVAL
                     CLERSADLVVAPLAVWKAGGAYVPIDPHFPASRIELMLEDSGATVIVTESGLLDRLPQ
                     NLPTVICVDRDRAMLARESREAPSPVTARNRLAYVLYTSGSTGIPKGVEIT"
BASE COUNT          518 a          791 c          742 g          518 t
ORIGIN      
        1 tgcgtgatct ctacgccttt tggaatgccc gtggatcccg acgtgtacag cacgtaggcc
       61 agtcgattgc gcgccgtcac tggggacggc gcctcgcgac tctcccgcgc cagcatagct
      121 cgatcacgat caacacagat cacggtcggg aggttctgcg gcagccgatc caacagcccg
      181 ctctccgtga cgatgaccgt tgcgccggaa tcctcgagca ttaactcgat tcggctggcc
      241 ggaaagtgcg gatcgatcgg cacgtaggcg ccacccgcct tccacaccgc cagcggggcc
      301 accaccagat ccgctgaccg ctccagacat aacgcaacaa gtgtttctgg accgactcca
      361 agcgtccgca accggttcgc cagccggttg gctcgctcgt tcacctcacg atagctcagg
      421 accttctgac cgcacacgac cgcaacggca tggggcctct tcatcgcctg ccgctcgatc
      481 cactggtcga cgctctcttc cgggaccgga atcgaagctc cgtggctttt cccgaaccac
      541 tccgcttcct gctcttcgct gaccagagca agttcgtcga tgggggtatc cggatgacgg
      601 gccatcgcag cggccagtgt cgcgtagctg cccgcccagc aagcgatggt gccggcgtcg
      661 aagagatgtc catcgtattc aatggaccct tcaaatcccc gctctgtctc ccagacgtac
      721 attgtcagat caaacgtcgt tcctccactg acgattcgaa actctccggc gtgtggagtc
      781 ttctgttcga tgaacgctgt ctggaacagc ggtgaacggg caaggttgcg gctgcgcacc
      841 agttcgctga cgacgcgctc aaacggaaca tcgctgtggg tgagcgcctc gaggaccgtt
      901 ccgcgcacct gctgcagcag ctgccttgcc gtcatccctg cgggaaagtc agcgcgcagt
      961 gcatgggtat tgatcaggca tccgatcagg cgttccagtt ctgggcgtgt ccgcgttgaa
     1021 accggtgtac ccaccacaat cgtctgctgg ttcgtgaagc ggtgcagcaa tgcctgaaat
     1081 acggccagca acgccatgaa cggtgttgcc cccgcctctg ccatgtggcc cttcagcagc
     1141 tttgatgttt cgctgcccag ttgcaccggt tgaagctttg cttcaaaggg cagagagaga
     1201 gaatgcggcc gatccgtcgg caactccagg tagggaggca catcccgcag tttttgtttc
     1261 cagtaatcca gctgggcacg aaacgcgccg gtggcttcct gctgtcgctc ccagacggcg
     1321 tagtcgccgt attgaatggg cagagctgga ggctgccagg gccatgcgcc tgtgcgttga
     1381 gcataaaagc ccctgagttc cgataagaga attccgaggc cccaggcatc gcaaagaatg
     1441 tggtgcacag taagaacgag ggattgctcg gtatcgctcg ctctcagcag cttgagacgc
     1501 aacagaggcc cgcgctgcat gtcgagttgc tgtcgcgaca tctcatcgat ttgctcgtct
     1561 atcgcgttct ggcgtttgct ttccggcaac gccacgccgt cccacacttc cagatgcggg
     1621 gcggcttcgt cagcaatcac ctgggtagga agtccgcggt cttcaggaaa acaggtgcgg
     1681 agaacctcgc gccggcgcac gacctcttcg agcgcccagc gcagcgcctc gacgtctact
     1741 gcgccggaga acggcagaac ggcagtgatg ttgtaggctg tgctctgcgg atcgagattc
     1801 tgcagaagcc agaaccgctc ctgcgcgaag gacagcggca gcactgtatc cgacgcggca
     1861 cgtcgtggaa tcgtaagcgt ggccggagcc ggcgctcgga ggacacgcag caactctgcc
     1921 ttggccagct gaatgcgctg ctccaggtcc ggtgtcagcc gtccttcggg tgcactgcac
     1981 cgcaaccggt caccgtcgac agtgagctgg atatcaagcg ttcgcaattc cgacagcaat
     2041 tgctcggcgt tcacaggcag gaagactcgc agatgaatcg atacgggaaa aacccggcag
     2101 aggagcctgg gggtgttctt ttacccctct ttaaaaaaat gccccgataa tcaacgcgtc
     2161 cagcaagccg ggatgcgaag tcctcccgct tgcactggag acattacggt ctctcttccg
     2221 accgcttgtg cgactttcat ccgtcgtcgg taacggttcg gatggctttt tgtgatcagc
     2281 gccgggctga gttttcgggt gaattgtcac atccgatctc aacgcaatcc gctgaaacac
     2341 aaggatcttt ttgcgcagac agtgcctgcc taaccttaca aatcattgtg tggaaatcaa
     2401 ataacacgac ggtaaccctc gccagcctaa cggaagcaca cacgaaaggg ctacaggact
     2461 acccggaatg gggatccatc tcgccgcgaa gaagcacata taatcgtggg cttatcctgg
     2521 cctgcgtttc attgtcatgg agaagggccg cgtggcagcc ctgcaggga
//
LOCUS       DASZYN010000446         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_984331, whole genome shotgun sequence.
ACCESSION   DASZYN010000446 DASZYN010000000
VERSION     DASZYN010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984331"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /gene="lepB"
                     /locus_tag="VHE33_11440"
     CDS             <1..348
                     /gene="lepB"
                     /locus_tag="VHE33_11440"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HVT98109.1"
                     /translation="PYIDDFPSIAPAPDNGSTAEWSVLMGQYLHNGEIEVPSNCYFVM
                     GDNRQKSYDSRYWGFVPRANILGRPLFVYWSIETPELPDNAPISQRASDTADELLHFL
                     TRTRWARTFHPVH"
     gene            536..>1639
                     /locus_tag="VHE33_11445"
     CDS             536..>1639
                     /locus_tag="VHE33_11445"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HVT98110.1"
                     /translation="MSAALHAVARPVICLLAAIPLAISPLPAQNLPDQALPSAAFVQT
                     LHAEYDRGEYRPHAPRESRWLDGGQRYTVLEASPSHPGATDLAVYDTETGQRKVLVAA
                     ADLIPKGNSQPLAIDDYQWSADGAQLLIFTNAQKVWRQRTRGDYWVFRIADKSLARIG
                     ADAPAASLMFAKFSPDGRSVAYVRANNIYVENLSDHQVRQLTHDGSPEIINGTTDWVT
                     EEEFALRDAFRWSPDSASIAYWQFDQSGVGEYTLINDTESAYPTVFRYRYPQPGGTNA
                     AVRVGVVSAQGGPTQWISVPGDPRNNYIPRMDWVGDSDEIVLESLNRLQNTNRVLIAN
                     AHSGATRLLFEDKDPAWVDIVDSFQWLSSKAGRR"
BASE COUNT          301 a          563 c          443 g          332 t
ORIGIN      
        1 ccctacattg acgacttccc ctccatcgca cccgctccgg ataacggatc gaccgcagaa
       61 tggtcggtgc tgatgggtca atatctgcac aacggggaga tcgaagttcc atccaattgc
      121 tatttcgtta tgggcgacaa ccgtcaaaag agctacgaca gccgctattg gggcttcgtt
      181 ccccgtgcaa atatcctggg tcgaccgctt tttgtttatt ggtccattga aacgccggaa
      241 ctgccggaca acgcgcctat ctctcaacgc gcctcggata cggcggacga gctactgcat
      301 ttcctcaccc gaacccgttg ggcgcgaacc tttcatcctg tccattgaca ccgggcatca
      361 gggcgtaccg ccactccctc tcccggatcc tttgcccgcc cacgatcatc cgcgacggcc
      421 gctagcgatc cctatctcat cccactctcc cgatccccgc tgctaccatt gggaccattc
      481 cctatagtct ctttccggct cctgtaaaac tgacgctccc aggatgagga tccgcatgtc
      541 tgctgcgctc cacgctgttg ctcgcccggt tatttgcctg ctcgccgcta ttccactcgc
      601 catttcgccg ttgcccgcgc agaacctccc ggatcaggcg ctcccgtcgg cggcttttgt
      661 ccagacgctg cacgcggaat acgaccgcgg cgaatatcgc ccgcacgcac cccgagagag
      721 ccgctggctc gacggtggcc agcgctacac cgtcctcgaa gcctcgcctt cccatcccgg
      781 agcaaccgat ctggccgtct acgacacgga aacgggccag cgcaaggttt tggtagcagc
      841 cgcggacctg atcccaaaag gcaactccca accgcttgcc atcgacgact atcagtggtc
      901 tgccgacggc gcccagttgc tgatcttcac taatgcgcag aaggtctggc gtcagcgtac
      961 gcgtggcgat tactgggttt tccgcatcgc cgacaaatct ctcgctcgga tcggcgccga
     1021 tgctccggcg gcctcgctga tgttcgcgaa gttctcgccc gacggccgct ccgtggctta
     1081 cgtccgcgcc aacaatatct atgtcgagaa cctttccgat catcaggtgc ggcagctgac
     1141 ccatgacggg tcgccggaga tcatcaacgg cacaaccgac tgggtcaccg aagaggagtt
     1201 cgctctgcgc gatgcgtttc gctggagccc cgactcggcc tccatcgcct actggcagtt
     1261 cgatcagtcg ggcgtgggcg aatacacgct gatcaatgac acggagtctg cttatcccac
     1321 cgtgtttcgc tatcgctatc cccagcctgg cgggaccaat gccgcggtgc gcgtgggcgt
     1381 ggtgagcgcg caaggcggtc ccacgcagtg gatcagcgtg cctggcgatc cgcgcaacaa
     1441 ctacatcccg cgcatggact gggtcggcga ctcggacgaa atcgtgctcg aaagcctcaa
     1501 ccggctgcag aataccaacc gcgtactgat cgccaacgcg cactccggcg ccacgcgcct
     1561 gcttttcgag gacaaggacc ccgcctgggt ggacattgtc gatagcttcc agtggctttc
     1621 gtcaaaggcc ggtcgcagg
//
LOCUS       DASZYN010000447        32552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_470618, whole genome shotgun sequence.
ACCESSION   DASZYN010000447 DASZYN010000000
VERSION     DASZYN010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 32552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32552
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470618"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VHE33_11450"
     CDS             complement(<1..488)
                     /locus_tag="VHE33_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98111.1"
                     /translation="MERRGFFPLGIYLYAGAAIYLGVLGLVSGDFAANWQRVTPDAPH
                     RMLLASAAGAWELLAGVAMLWRRMSRWGALALAGIYSIFWLLWAVHAASAPRIWDSWG
                     NFFEEASLVIAGLAAFAWLSPAGSAWPRWAVTIARLYGVCCLCFGATHFIFLSGAGNF
                     VP"
     gene            complement(653..2083)
                     /locus_tag="VHE33_11455"
     CDS             complement(653..2083)
                     /locus_tag="VHE33_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98112.1"
                     /translation="MRRLVPLLLALAGAAAAPAQTPSTAPGNESRVASDFRREWAQLH
                     PCSGKPAGTCKSGPGDLVDIGQVLFIGQPLHVAVGSLAPQNGFGMGLAFVEHTDFSNE
                     WRLTYNADGVATPNGSWRAGFYLKAYRLGGGTIVPVAGPGTKQSSLFHTAPLFNAYVE
                     TTSLNKLYYYGLGPDTTRAGQSVFGMTETIAGANAIVPVGAASLSILGEINGRIPRVR
                     GNQGESVPSIEQIYSNATAPGLSSQPSFFEPGIGARIRPTLLGGYLRLNYLAELQDYR
                     GGAGSSFRRWTADLHHEIPMDTRVRLKAASDQNGPDSCAPTAQEPCGSPLHVSSAINH
                     EGSVGFRLLMIGSAASTGNAAPFYFDPTIGGSDINGQALLPSYPDYRFRAPNVMLLTE
                     SVEHAVPKLPLGVYFSVDEAKAALTRSDIDFSHLRRSYTAGLTVHAGGLPLVYLLFAW
                     GGDEGHHITFNVSNVLLGGSSRPSLF"
     gene            complement(2080..2490)
                     /locus_tag="VHE33_11460"
     CDS             complement(2080..2490)
                     /locus_tag="VHE33_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98113.1"
                     /translation="METIVVPAPPKSAFNKKRPVSDLVRKQVEHFQHVAQKHGMKIDP
                     ALERDLATEGGAARYIARVTREMRGQSERTPGPRLVARPPGTAKSKPAAAGLDLAAAA
                     DRGRKPGAGKATKDAGRTPKKSSSGGKGGGKRKR"
     gene            complement(2517..3740)
                     /locus_tag="VHE33_11465"
     CDS             complement(2517..3740)
                     /locus_tag="VHE33_11465"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVT98114.1"
                     /translation="MALDRSLPGIQQLKHIVVLMMENRSFDHMLGALKAENAKIDGLD
                     GTESNPDNTGAAVKVQPLAAYQSQLDPDPNHHFAAVNLQIFNDPQKGPPLPATMQGFV
                     KSYFNQQQNTAHSAKILYYFTPDKLPVLTTLARNYAVFNRWFASIPGPTICNRAFAHY
                     GTSFGQVSMDLFYEGKEWKAIYDRMLAANHTCKLFYYDEASSTMEVVNLLKNQPAIFG
                     TWEDFKTACDKNALPDYCFVEPNYTDHDAPDGSGEAIACDQHPDHDVRAGEQFIADVY
                     NHIRQNPKLWPNTALLIVYDEHGGIYDHVPPPSCMPDGFVAQPKDTGTLDAFHFDRLG
                     VRVPAILVSPWVEKGSVIDDVFEHASIPGTVTDYFLGTYDARSPREKAANNFLEYLSR
                     PTVRAEDDCPSFKLK"
     gene            4067..5608
                     /locus_tag="VHE33_11470"
     CDS             4067..5608
                     /locus_tag="VHE33_11470"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HVT98115.1"
                     /translation="MPFSRRTFVNLGLAATAESIARRSRLYASPLASVPAAELAADPR
                     RPQFHLLPAANWMNDPNGPIYWRGQYHMFCQYNPNAAVWGDMHWAHAVSPDMLHWRHL
                     PIALAPQPHGADAAGCFTGTAIRDGNRVVVLYTGVIDTAPPGSSPGFKESQCIAIASD
                     PDLTQWNQDPDPVIAAPPPGLDVTGFRDPTPWREGNTWYLAVGSGMRGQGGAVLLYRS
                     ADLRHWEYLHLLAHGVPTSSSDHDPVASGDMWECPDFFPLGDKHVLIYSTQGKTFWQS
                     GTLDTAAMLFHSERSGILDYGSFYAPKTQLDRLGRRILWGWIPETRPVAEYRAAGWAG
                     LMSLPRVLTLDNNNDLRVALLPEFDQLRRPERRLRLTGDAAQAGQQLAGLTLREACGE
                     IVISFREESEPLQFALVAAQNPATSWLTCSYDPSSPGHISIDDHPLPLGAGDSAASVE
                     LRFVMDGSVIECFANNSSTLTKRFYYPGPMAPAIGIDLKSSIKNLTRLSIAQVAPISA
                     NRLTT"
     gene            complement(5656..8490)
                     /locus_tag="VHE33_11475"
     CDS             complement(5656..8490)
                     /locus_tag="VHE33_11475"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HVT98116.1"
                     /translation="MTPRMLRITCALLISMVAGLAAAQTIHLSTSKEIVGPVPGSPQP
                     LNSLPMATAWSPDHRYLALVNAGFGTVESNYEQSIAILDTTTGKLTDYPEPRTEVQQP
                     QTYYSGIAFSADGSHLYVSFDSLSEPEGGKPDETGNAIGVYGFAPEASATDVLKPERL
                     IPVPMRELTGGQKQNHGPTDLGEDKAIPAPAGIAVVKGEDGAEKILVADEYSDDVLLL
                     DTASGKLETRFDLSSGPVVPSTYPVAVVASKDGRRAWASLWNGSAVAELDLKSGKVVQ
                     QLPLLAPSRPVSPSSHPMAMTLSPDEKMLYVALANRDAVAAVQVSGQTMRLVTLYDTR
                     LPGQAFFGAMPDGVAISDDGATLYAANSGSDAVAVFNLKAGGKDAHAIPSGFIPTEWY
                     PTAIATQGDHLYVATGKGKGTAPNGAPQPAAPNPPANAPMRLEGRAHTYIASMLHGSL
                     ATIDVNEVKAHLPELTKEVLASNLMNAAQKKIGFEGGGDKIKHVIYIIKENRTYDQIF
                     GDLGAGDDDPSLTMYGEDITPNLHALAKQFGVLDNFYDSGEVSGDGHVWSTAAINSDY
                     NERTWQIAYRGHERPYDFEGVVEDGYPLLENIPDIDEPGSGYLWTDLARSHKSLYHFG
                     EFISTKFCDDSGEAPKDRPMPQAGGTPEGEHYCEHAFIRKGDPIPANYGGGTSEYPWA
                     IPLIYKNVAVKPELVGHFDPEYPDFNLSFPDQLRVNEFLTHFKEWTTDLKAGHDTMPA
                     FVMLRLPNDHTAGTRAGMPTPKASVADNDLAVGRAVDAVSHSAYWDSTAFFVLEDDAQ
                     DGADHVDAHRSISLVISKYAPRKEEPVVDHTFYTTVSTVHTMLTLLGCPPMNNNDAFA
                     PLMAPEFSGAGDQPAFSADYRNRDNGLIYTANAPKAEGAKESAKMDFTREDRADPVKL
                     NVILWKDAMGSKPVPWMLTHPHGVRKDDDD"
     gene            8825..9676
                     /locus_tag="VHE33_11480"
     CDS             8825..9676
                     /locus_tag="VHE33_11480"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HVT98117.1"
                     /translation="MPKLGRKHQSGMNWFTTVFMVIFHAGAIAALFMFTWKALICALV
                     LWVLATNVGIGMSYHRLLTHRGYRVPKWLEYALAVCGTLALEGGPVFWVSLHRVHHQL
                     SDHEGDPHTPHEGGWWAHAGWILIGDSLSGETESLARYVPDLMRDRFMALLSKYHWVP
                     QVIAAFVCFAIGGWPAVMWGIFLRTTIGLHATWLVNSATHMWGSRRFKTRDDSRNNWW
                     VALITGGEGWHNNHHAHPVSARHGLRWYEFDINYYGIWLLEKVGLARQIQVAQYDPAN
                     PRPAGTA"
     gene            9709..10683
                     /locus_tag="VHE33_11485"
     CDS             9709..10683
                     /locus_tag="VHE33_11485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVT98118.1"
                     /translation="MKVLAMQASRGHFVFGPMRLSGRRRNMVLFTLLGVCLIGVAVVL
                     NVGWIVLNWRRIVPMAIGIPFFALLIAGVALNTVFLVREVRRNERHDAFLSAVTHELK
                     TPIASIHLYLETLQRRHIDDAQRAEFYSRMLADSERLLATVEQVLKAGEVGQRARSDI
                     RVSVDMHALVEECIRTTLQRHHLEEKSIGLRVDEAQLPMFVRGDPNELQTALLNVLGN
                     AVKYSPDGANIRVALSIENDAWVRVSVTDQGIGIPAAHLKRIFRRFYRVPTRTVLGTK
                     GTGLGLYLVRTIARQHGGDATAESEGEGRGATVSLQLPRILTPLVGQQ"
     gene            10687..11415
                     /locus_tag="VHE33_11490"
     CDS             10687..11415
                     /locus_tag="VHE33_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT98119.1"
                     /translation="MARILIVEDETNLAQGLLFNLQAEGHQVSVESDGESALNCLRRE
                     PFDAVVLDVMLPGKNGFEVATALRAEGNYVPVLMLTARARSQDVVSGFAAGADDYLPK
                     PFDLNVLLARLNALLRRMQWPRAPEKRPARPDDPSAFDFDGRHIDFEALELHAHGRTV
                     RLTLMESDLLRHLTRNRERIVSRSELLEQVWHVREDTDTRAIDNFIVRLRRYIEDDPA
                     APKYLQTVRGVGYRFVPNPEQAHD"
     gene            11473..11682
                     /locus_tag="VHE33_11495"
     CDS             11473..11682
                     /locus_tag="VHE33_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HVT98120.1"
                     /translation="MMKEVTLRVENMHCGACVRRVSQALEKVGGVTVDEVRIGAARVR
                     ASDDVPETSILSAIEKAGYPAHLEH"
     gene            11813..14092
                     /locus_tag="VHE33_11500"
     CDS             11813..14092
                     /locus_tag="VHE33_11500"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HVT98121.1"
                     /translation="MHAAEKAKTETTPAIPANGNVRTITLPVIGMSCAACQSHVERAL
                     RSTSGVTDATVNLLTNSARVSFNPAIAKPEDLIASVRESGYDATLPVDPNSAAPAETA
                     AAEGPLRAKAIFTLAGGVLVMLLMGAHHWFAPAAPLLFAISPRTLEYIMLAITLAGMI
                     WGGGIIYRQAWTAAIHGGTNMNTLVALGTGAALAYSLAATTMPGLFRRHGLHPDVYYD
                     AVLLILGFLLLGRWLDARAKRRTLDALHEFMKLQPRSARILRNGQQIEVPLAEVAAGD
                     TVVLRPGERVPVDGVVLSGVTSIDESLMTGESLPVTRKPGDRVIGGSLNFDGAIEYRA
                     TSVGADSVLGQMMRLVEEAQSSRAPMQQLADRVSAIFVPIVLALAAATFLIWIFASGG
                     SGRAFAVAVAVLVIACPCAMGLAVPAALTVAIGRGAQLGILFKGGESLERLARIDTLV
                     LDKTGTLTEGKPEVVAVQALNGTSEDEVLSLAAALEQRSEHPLAHAILDAAARRSLKI
                     PAADEVRALPGKGIQGFISGQRIAAGNSVLLDDLGVPIPTANSAEPGTTTMYVARGSS
                     VVGIIQARDTLRATASDAIAGLRRLGIRTAMLTGDTSATAESIARAAGIDQVWAGLLP
                     DQKLAKIRELQSQGHRVAMAGDGINDAAAISQADAGLAMGTGADLAREAGDAILLHGE
                     PRQILDAVLLARQALRVMRQNLGWAFGYNLLGIPIAAGVLYPAFGILLSPAIASAAMA
                     LSSVSVLTNSLRLRRFTPR"
     gene            14102..16420
                     /gene="lptG"
                     /locus_tag="VHE33_11505"
     CDS             14102..16420
                     /gene="lptG"
                     /locus_tag="VHE33_11505"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HVT98122.1"
                     /translation="MRILTRYILKEVASHAAIGGAIFTFVLFIRYLPHLLEMIVRNSA
                     SLGSVFEIVALTLPDMCTVTIPMAVLVGILLGLSRLAADSEITAMRASGIGVWSFVRV
                     VGVVSVVAWGVSLFNTLYLAPKSAAAMRRLEESLANAQASFEVQPRVFYEDFRDYVLY
                     VQDVRSGTGAANWRGIFLADVTHPNAPKITTADRATVVNSRNQAMTMRLRDGTESQVV
                     PDQPSQYTISTFGQTDLPLQTGGQEDVRLGRSDTPILAMSTRELLTRSHGRNGSIYQI
                     EFEKRLAYPAACIVLMLVGVPLGISSRRGGKGAGFVLTIALVFVYYFLSSTGTALARQ
                     HKVPAFLGVWQANIVFAVCGVVLLRQMATGGAALSALTSLGNWFRLRAEESASSAGPV
                     KATVRKRAPRGRFPLIFDEYVLREFLTTFVMVLVTFVMLMLIFTFFELLSDIIRNKTP
                     LITVGAYLINLTPSMIYLITPLSVLIGVLVVFGVMNRNSEMTAMKATGVSLYRITVPV
                     VAIAAILSVSLFMFDELYLPQANRRQESLRNVIKGKPAETFQHPGRKWIFGQQRPGQP
                     GRIFYYEYFDPDLDTFGNISVFEFAPGSFTISRRIFASRAHWEPSLNTWVFEDGWDRT
                     FSGAEITSYESFPVRTFPEISEEPSYFKKEVRPSSEMSFGELDDYIHDLRQSGFNTVP
                     LRVQLNHKLAYPLITLVMAVLAIPFALSMGKRGSLSSIAIAIGVAIAYFVVSGFFEAM
                     GNVSWLPAFLAAWSPDLLFGLAGAYLLLRTPT"
     gene            16436..18199
                     /locus_tag="VHE33_11510"
     CDS             16436..18199
                     /locus_tag="VHE33_11510"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT98123.1"
                     /translation="MFRNLQPLLPYMRRYWRGYLWGCLCTVLANAIWVQFPRVIGIAV
                     DDLTVRVTWNKIFSYAGMLLAVTVAHGILLFLMRWIIIGISRDIEFDLRNDLFAQLEK
                     QSASYYHDHRTGDIMARMTNDLNAVRMLLGPAIMYTANTVLFFAGALYFLLRISPMLT
                     LMALAPMPLASILIQFMGRRIHDRFERIQAMFSDISAQAQENFSGARLVRAFAQEEAQ
                     VKAFERSNQEYIRRALRLVRLMGMLWPTLEFMLGLAMVISLLVGGHLVLQHRIPVGEF
                     VAFTTYMLMLTWPIIALGYVVNLFERGTASVVRIHELLTAKPTIDDRDAVLSSEGSEI
                     HGAIEFRDLHFAYPASNGDPVEVLHGISLRIPAGSSLALVGPTGSGKSTLVSLIPRLY
                     DAPPGSVLIDGQPIRAWRLDSLRAAIGFVPQETFLFSNTIHENIAFGVPGASVAAVAE
                     AAQSAHIRDEFDLFPNRFETTVGERGLTLSGGQKQRTALARAILRNPRILILDDALAS
                     VDTYTEEQILEELRSVMAGRTTILISHRISTVRHADRIGVLVRGQIVELGTHEELLAL
                     NGYYASLFQKQLLEEELAVTH"
     gene            complement(18205..18666)
                     /locus_tag="VHE33_11515"
     CDS             complement(18205..18666)
                     /locus_tag="VHE33_11515"
                     /inference="COORDINATES: protein motif:HMM:NF014604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HVT98124.1"
                     /translation="MNYQQQAIAGMNPVELIVALYDGMMRFLRNAIDAVERRDTAARR
                     TAIGRVLEILMHLQSRMRPDVGGDSAKVLSEFYAAVFALCLEGSRLESADRFREAIGC
                     IRDVREAWAVAARDPEVLRLIGEDAEPQMVATAATHLSAGFDEPAASSWTA"
     gene            complement(18697..20121)
                     /gene="fliD"
                     /locus_tag="VHE33_11520"
     CDS             complement(18697..20121)
                     /gene="fliD"
                     /locus_tag="VHE33_11520"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HVT98125.1"
                     /translation="MGSVGLSFGSPTSGEGFDVSATVDKIVTNLQAVETPWKNQLTTL
                     GAQDTALTSMGTDLSSLATALQTLTDFQGTFSGKEGSSSDNSVLALMNATSAAAAGSH
                     TIVVSQLAKAFSYASTAVNSGDTLSGTLKIGSQTISLSDGTVRDPSSGALIPQNNTLA
                     TLASFINAGQYGVQANVVPVGTGQSELTLISTTSGAAGSVTLDASGLSDATTGSSTAG
                     GVSFSRQQKGQDAQFSVDGLPAMSSSSNTVTTAIPGVTMQLLSAPPGNSVQVEITNNN
                     SQVESDINAFVNAYNKVIGDLNTQEANSSSGTAEPLYGNPTVAMLQEQLQQAIGFVQS
                     SGAVTALSQLGITPSTKYDGTLTLNTATLDTMLNSSYQDVMNFFQPSGSYTSFGKNLT
                     TALNNAGSSGPHGAIYLALQQNSAQEKALNQNISNQEELIDAQKKQLTTELNAANFIL
                     QEIPQQIEYVNQIYSAITGYNQQR"
     gene            complement(20270..23428)
                     /locus_tag="VHE33_11525"
     CDS             complement(20270..23428)
                     /locus_tag="VHE33_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HVT98126.1"
                     /translation="MPLGVLNNISAIYAENNLNTTQTNLQNTLQQLSSGSRINSGADD
                     SAGLAIANGLQANSSALTQSSANATAGVGFLQVADGALSQVTNLLNRAITLATEAGGG
                     TLTASQMSAADQEYQDILNQINTIGSTTEYNGIQVFGADKTASALTWSSVLGGTQTNT
                     AAATSASATAAALAGGNIVAGGTTAGVNATQGGTFAASPTALTWTAGGAGAYSVITTG
                     PIVNGSQLTGTFGFTPTASSGSPNAISVNLGTAITSTNLTTAASQLQTAMNTQAGGAD
                     YTVSVVNGNQLQIGFGAHASADHLTGFPVISNTGSAAAAQSAGTYQLTAANGDSLSGT
                     LTLTALTNTAAGAASNVVWNGQGTASVSGTIASVNGNPDALSGTLTLTTNAGVTGAGA
                     VSNLVWAANGTTIAAGDVLAGGITIALTGGTVDGTSLDVDLTGLTAGHDAASDTAFQT
                     AIKAQITAAGGTAGDYTVAYNHTTGALTLAVNGTPADGLTSFAVSGTSATHGASSQHP
                     TTGTLAATINLNGVTTGNLANTIAAGLAAQSGMSVNDWHNDFTVSYSNGTLTIATTAG
                     GTGHGITSITASGLQQATSATATALTPTTIDLNGTNTANLASVVSQALNGNVSNPNYT
                     VAYNSGTGVLSVGLTAAATANNITGISWNAAPSGNITQTAANQASLTFTSNEVLGGQL
                     TINQTIAGQAQSAITLNLSNGINTAHNAADLVTAITTALGNNAQYYTVSANTVNQNTT
                     VSMAINQVGINANVDSIAITNGTGSNALSQSQITEANITGSAVMGGFTLTPTGSSSTP
                     IVVNLAGESTDNTDLVSALQAALGSNYLVGLTPVNQPNPNQNNNGAPAPLNDNLTIGV
                     SAAGVAAGITGFTIANASTNGIEQLSQSTNGVNIYTSDGTSAGSKNYNVTIGALSQSS
                     VGSSALNSVQGTQITATVGGVTGTGGTSAGATVGTSLSGTSLASQANAEAALQTVTNA
                     ISAVAYQRGQVGANINTLTAASNIASSEQTNITSAQNAITATDYASATSNMSKYEILT
                     QTGISALAQANSTQQMVTKLLQ"
     gene            23656..23961
                     /locus_tag="VHE33_11530"
     CDS             23656..23961
                     /locus_tag="VHE33_11530"
                     /inference="COORDINATES: protein motif:HMM:NF018043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HVT98127.1"
                     /translation="MIPLTRLNGNSLVVNSDLIKCAESSPDTMLTLINGEKIVVLESC
                     DEVVRRTIAWRGSVLAEAATHLQAVPWTSTAILPGAENSEASATASSPDTRRANSEA"
     gene            23965..24765
                     /locus_tag="VHE33_11535"
     CDS             23965..24765
                     /locus_tag="VHE33_11535"
                     /inference="COORDINATES: protein motif:HMM:NF006583.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HVT98128.1"
                     /translation="MDAGSLGGLLLALAGILAGLLFEGGNLSQILQPTAAMIVFGGTL
                     GAVMLQFPFRIVRSAALGLVRVFFHRGGGAAALLRTIVAFANKARRKGIVSLDQDLAQ
                     VTDPFLRQALMLAVDGTEPSELRRIMEVQIDAVSENDARIPAVFESAGGFSPTIGIIG
                     AVIGLIQVMQHLDHMDEVGRGIAVAFVATIYGVGAANLFFLPAAGKLRQRAREDQRLR
                     EMALEGVISIVEGMNPRMIEIKLAGFQGSAAAPSVNSDQPRLVAEKRA"
     gene            24762..25547
                     /locus_tag="VHE33_11540"
     CDS             24762..25547
                     /locus_tag="VHE33_11540"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HVT98129.1"
                     /translation="MNSGRLKSRRTSDHVHHDRWLVSYADFITLLFAFFVVLYASSKA
                     DQQKQSQIAHSIRSAFEALGVFPSPPRADSHAPPDSQASIPPVNVVLGDDLAASPEIQ
                     QDLHRLQNELAATLAAQVAQHNIALKLGRDGLTISLRESGFYDSGSALPHSQSRPTLE
                     KVAATIGATPYDIRIEGHTDNVPIHTAQFASNWELSTARATELAKLFIVNYRFSPARL
                     SAAGFAEYHPVAGNDTPEGRSQNRRVDIIVLPRISVVPTQNQH"
     gene            complement(25686..26684)
                     /locus_tag="VHE33_11545"
     CDS             complement(25686..26684)
                     /locus_tag="VHE33_11545"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVT98130.1"
                     /translation="MEYVNLGRTGLKVSRICLGCMTYGRPPAPGEPQAGRHAWALNEE
                     ESQPFLKQALDLGINFFDTANVYSSGASEEVLGRFLKKNVRRESVVVATKVWGVMREE
                     PNGRGLSRKEIVFELDESLKRLQMDYVDLYQIHRWDEETPIEETLEALHDVVKAGKVR
                     YIGASSMQSWQFAKALYLAEKHRWTRFVSMQNHYNLLYREEEREMIPLCREEGIGVIP
                     WSPLARGRLARPWQSETTKRSETDRFGNTMYSKTEEADQKVVEALTALAKKRGVSQAS
                     LALGWMLAKPGITAPIVGATKRNHLEDAVAALAVKLTSEEMAALEAAYVPHPVLGF"
     gene            26864..27586
                     /locus_tag="VHE33_11550"
     CDS             26864..27586
                     /locus_tag="VHE33_11550"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98131.1"
                     /translation="MSPARRLRILYDRLVETWGPQQWWPAQTPFEVVIGAYLTQNTAW
                     KSVERSIANLRSRRVLSVSGLRNLPEAELRDLIRPSGFMLRKAAALKAFIVFLDAHHN
                     GSMRALQRAALLDASAVRKQLLDLPGIGPETADAILLYALKVPAMVVDEYLRRVAIRH
                     GLVPHHAKYAEIQTLAEQAFVPERSSENSDELARHYNEFHALIVETGKLHCRRIPLCE
                     GCPLAFDLKNPPFSYPVHKMYG"
     gene            27583..28209
                     /locus_tag="VHE33_11555"
     CDS             27583..28209
                     /locus_tag="VHE33_11555"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HVT98132.1"
                     /translation="MTVTLYAATTNPGKLRDFSVAAADAGFLVESAPRLRAIPAPEEK
                     ELTFAGNARLKAQYYSRLLPGFILADDSGLEVDALGGEPGVRSARYADDAGFHPKGSG
                     PTLSTDERNNLYLLSRMLGVTDRRARYRCVLAVACEGELLLTGEGTVSGEILAAPRGA
                     GGFGYDPLFNIPQLLLTMAEISLEQKHTLSHRGRAFRALLPGLKKLSG"
     gene            28327..29154
                     /locus_tag="VHE33_11560"
     CDS             28327..29154
                     /locus_tag="VHE33_11560"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVT98133.1"
                     /translation="MRIDAHHHFWHYTPDEYGWINDAMSVIRRDFLPSDLQAEISAAG
                     IDAVISVQACHTIAETDWLLAKAAAHPWIAGVVGWLPLADPGIGALLERFSANPWLKA
                     IRHVLQAEPDAYMDRPDFNNGLAQLRALPLTYDILIYHSQLPAAIRLVDRHPDQVFVL
                     DHIAKPAIRAGELQPWAQNLAELARRPNVFCKLSGVVTEANYQTWTYDQIRPYLEAAL
                     EAFTPSRLMFGSDWPVCCVAVSYVDWVRTIERFTTALSATEREAIFNSTAASAYRLD"
     gene            complement(29224..30468)
                     /gene="glp"
                     /locus_tag="VHE33_11565"
     CDS             complement(29224..30468)
                     /gene="glp"
                     /locus_tag="VHE33_11565"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HVT98134.1"
                     /translation="MTPEESPSIVLSYDHAASVVHRQAVRAREEASRKPNVEKVPLGE
                     AAGRVLAEPVVADRDQPPFPRATRDGYACRLADLDAGGLRVVGLLRAGEAWTLGAIRP
                     GEAVEIMTGAAVPPGADCVVMVEHVVSGEEHISLAEGREMTAGENIVPAGAEARAGDV
                     VVPAGGRIGIAEVAAAAACGAAQLAVFGRPRVAILATGDELVELDRKPAPHQIRNSNS
                     YSLAAQVRAAGGEPIVLPPVHDDLAATERAIRDARDCDLIVLTGGVSMGKYDFVEQAL
                     VRLGSEFYFTGAKIQPGKPVVFGMLGDTHFLGLPGNPISTIVTFTLFGAPLLRALGGE
                     SDERPRFTLARLEKEVRVKPGLTRFLPAIVVSSFRGVHVRQIGWQGSGDLAAAARANG
                     FLVVPDTVERLAAGEIVTVLEI"
     gene            complement(30542..31642)
                     /locus_tag="VHE33_11570"
     CDS             complement(30542..31642)
                     /locus_tag="VHE33_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98135.1"
                     /translation="MRMKHVAPRVALAVLLSTPLFLGAQTTTGKIHGHVSDPAGTPET
                     GGTVSLYEGGLASANATPKYTFDVDANGDFKGELAPGSYTMTYRQANTPKDKVADQID
                     NIHVTAGQDTEANDDMSRPDYLAKLSPEERKALEEAKSKNASILKENAQIKNLNADLA
                     KSRSDRAAKNFSDAAALMQKDVTAKPDEPLLWVELGEAQLGLKQYGDAETSLKKGIEL
                     EQASKKPNNEVIAAAGNALGEAYGSDNKIPDAQAAYDEAVKANPPQATMYYTNEAIIM
                     DRNNQIDATVAAADKAIAADATKPIPYYLKGKALLNKATVDPKTQKIVAPEGCQEAYK
                     KYLELAPTGPFAADAKSVLAELSATPSSGKKH"
     gene            complement(31671..32423)
                     /gene="queC"
                     /locus_tag="VHE33_11575"
     CDS             complement(31671..32423)
                     /gene="queC"
                     /locus_tag="VHE33_11575"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HVT98136.1"
                     /translation="MSQADESSHRERAVVCLSGGMDSCVCAALAARDFDAYALHCSYG
                     QRTEEREAQAAHAVADALGFRDFLRLKLDLFRRIGGSALTDERIEVPEAPTDSATIGA
                     EIPVTYVPFRNAHFLSAAVSWAEVLGAKTVLIGAVEQDSSGYPDCRPAYYGAFQELIH
                     QGTREGDIIVRTPLIHMQKKQIVALGLELAAPLELTWSCYSGSEVACGVCESCVLRLR
                     AFEQAGAKDPIPYATRGWTGAQAGETSQEGAA"
BASE COUNT         5564 a        11760 c         8727 g         6501 t
ORIGIN      
        1 ttcggcacaa aattccccgc acccgacaag aagatgaagt gcgtcgctcc gaagcaaagg
       61 cagcacacgc catacagccg cgcaattgtc actgcccagc gcggccacgc cgaccctgcc
      121 ggtgaaagcc acgcaaacgc cgccagcccc gcgatcacca gcgacgcctc ttcgaaaaag
      181 ttcccccagc tgtcccaaat ccgcggagcg cttgccgcat gcaccgccca cagcaaccag
      241 aagatcgaat agatcccagc cagcgccagc gcgccccagc gcgacatccg ccgccacagc
      301 atcgcaaccc cggccagcag ttcccatgcc cccgccgcag acgccagcaa catgcgatgc
      361 ggcgcatccg gcgtcacccg ctgccagttc gcggcgaaat cgcccgacac caggcccagc
      421 acgccaagat aaatcgcggc gcccgcgtac aaatagattc ccaaagggaa gaagcctcgg
      481 cgttccatcg gtaagcgctc ctgttccaaa cgaatcgagg ccccgtctcc gcaggcttcc
      541 tctaaaagtt tgagtgcagt ctcgccgcca gctcacccca cccgccataa ccacttttcg
      601 cccgcgcccc atgccactgc aaggagcgaa cccgaagccc agcgcgaaaa gactagaaca
      661 gcgacggtcg cgacgatccg cccagcagca cgttgcttac attgaacgtg atgtgatgcc
      721 cctcatcgcc gccccaggcg aacagcagat acaccagcgg caagccgccc gcatgcaccg
      781 tcagtcccgc cgtgtaactc cgccgcagat ggctgaagtc gatgtcgctg cgcgtcagcg
      841 ccgccttcgc ctcatccacc gaaaaataca cgcccagcgg caacttcgga acggcatgct
      901 ccaccgactc ggtcagcagc atcacgttcg gggcgcggaa ccggtagtcg ggatagctcg
      961 gcagcagcgc ctgcccgtta atatccgacc cgccaatcgt cggatcgaag tagaacggcg
     1021 ccgcattgcc cgtgctcgcc gctgacccaa tcatcagcag ccggaaaccc accgagcctt
     1081 cgtgattgat cgcgctcgac acgtgcagcg gcgatccgca cggctcctgc gccgttggcg
     1141 cgcacgagtc aggcccgttc tgatcgctcg ccgcctttag ccgcaccctc gtgtccatcg
     1201 gaatttcgtg atgcagatcc gccgtccacc gccggaagct cgatcccgca ccgccgcgat
     1261 agtcctgcag ctccgcgagg taatttagcc gcagatatcc acccagcagc gtcggccgga
     1321 tccgcgcacc aattcccggc tcaaaaaacg acggctgcga actgagtccc ggggcggtcg
     1381 cgttcgaata gatctgctcg atcgacggca ccgactctcc ctgatttccg cgcacccgcg
     1441 gaatccgtcc gttgatctca cccagaatcg aaagactcgc cgcacccacc ggcacaatcg
     1501 cgttcgcccc ggctatcgtc tccgtcatcc cgaacacgct ctgccccgcg cgcgtcgtgt
     1561 ccggccccag cccgtagtag tacagcttgt tcagcgacgt cgtctccaca tacgcgttga
     1621 acagcggcgc cgtgtgaaac agcgacgact gcttcgtccc cggccccgct accggcacaa
     1681 tcgtgccgcc gcccagccga tacgccttca gataaaaacc cgcgcgccac gatccgttcg
     1741 gcgtcgccac accatcggcg ttgtacgtca gccgccactc gttcgaaaaa tccgtatgct
     1801 ccacaaatgc caggcccatc ccgaagccgt tttgcggagc cagactgccc acagcaacat
     1861 gcaacggctg cccgatgaac agcacctgtc ctatgtccac caggtcgccc ggcccggact
     1921 tgcacgtacc cgccggcttc ccgctgcagg gatgcagttg cgcccactcg cgccgaaaat
     1981 ccgatgcaac ccggctctcg ttgcccggag ctgtacttgg cgtctgcgcc ggtgcggccg
     2041 ccgctccggc cagagcaagc agaaggggga caagtcttct catcgtttcc tctttccgcc
     2101 gcccttgccg cctgaagaag acttcttcgg tgtacgcccc gcatccttcg tcgctttccc
     2161 agcacccggt ttccgaccac gatccgcagc cgcggccagg tccagtcccg ctgctgccgg
     2221 tttgctcttc gctgtgcccg gcggacgcgc caccagcctg ggtccaggag tccgctcact
     2281 ctgcccgcgc atctcccgcg tcacccgcgc gatgtatcgc gccgccccgc cctcggtcgc
     2341 cagatcgcgc tccagtgccg gatcgatctt catcccatgc ttctgcgcca catgctgaaa
     2401 atgttccacc tgtttccgga ccagatccga aaccgggcgc tttttgttga acgccgactt
     2461 aggcggcgct ggaaccacaa tcgtctccat caagctcctc cttgctgtcg cccgcgttac
     2521 ttcagcttga aactcgggca atcgtcctcc gcccgcaccg tcggccgcga caaatactcc
     2581 aggaagttgt tcgccgcctt ctcgcgcggc gaccgcgcgt cgtacgtccc cagaaagtag
     2641 tccgtcaccg ttccgggaat cgacgcatgc tcgaacacat cgtcgatcac cgaccccttc
     2701 tccacccacg gcgaaaccag gatcgccggc acccgcacgc ccagccggtc gaagtggaac
     2761 gcatccagcg tccccgtgtc cttcggctgc gccacaaaac cgtccggcat gcagcttggc
     2821 ggcggcacgt ggtcatagat cccgccgtgc tcgtcataca caatcagcag cgctgtgttc
     2881 ggccacagct ttggattctg ccggatgtgg ttgtaaacat cggcgatgaa ctgctcgcct
     2941 gcgcgcacgt cgtggtccgg atgctgatcg cacgcgatcg cctccccgct cccgtccggc
     3001 gcgtcgtgat ccgtgtaatt cggctccaca aagcagtaat ccggcagcgc gttcttgtcg
     3061 cacgccgtct taaagtcctc ccacgtccca aaaatcgccg gctgattctt gagcaggttc
     3121 accacctcca tcgtggagct ggcctcgtcg taatagaaca gcttgcaggt gtgattcgcc
     3181 gccagcatcc gatcgtagat ggccttccat tcctttccct cgtagaacag gtccatactc
     3241 acctgaccga acgacgtccc gtaatgcgca aaggcgcgat tgcatatcgt cggtcctgga
     3301 atcgatgcga accagcgatt gaacaccgcg tagttcctcg ccagtgtcgt cagcaccggc
     3361 aacttgtcgg gcgtgaagta gtacagaatc ttcgccgagt gcgccgtgtt ctgctgctga
     3421 ttgaagtagc tcttcacaaa gccctgcatc gtcgccggga gcggcggccc tttctgcgga
     3481 tcgttgaaga tctgcagatt cacggccgca aaatgatgat ttggatccgg atcgagctgc
     3541 gactgatacg ccgccagcgg ctgcaccttc accgccgcgc ccgtgttgtc gggattcgac
     3601 tcggtgccgt ccagcccatc gatcttcgcg ttctccgcct tcaaagcacc cagcatgtga
     3661 tcgaacgagc ggttctccat catcagaacc acgatgtgtt tcagttgctg aatgccgggc
     3721 aggctccgat ccagcgccat aacttaacct cgggaaacaa aaggtccgcc gcgcgggaac
     3781 gggaaagggg ctggacctga aaggccagtc acgggctcga ccgcagttct taccgactga
     3841 gctcgcgtga aggggagtgg gcaaaggaca tcgcgacgac atccgcaggg cgtcaacagg
     3901 ggagagcgtg tcacgcatca tagtccgctc ccgcgcctgc tgacaatcgt gttgcgaacc
     3961 cgcgccgcgt ttcacataag actcgccgca cagcaagaag ttactgcagt cctattcgcg
     4021 aaacccgaac ttctcgcgtc ctgaactaaa attcagcgag cccgccatgc ccttcagccg
     4081 ccgcacattc gtgaacctcg gcctcgctgc aacggccgaa tccatcgccc gccgcagtcg
     4141 cctttacgcc tcgccactcg catccgtccc ggccgcagaa cttgccgcgg accctcgccg
     4201 tccgcaattc catctgctgc ccgccgccaa ctggatgaac gaccccaatg gccccattta
     4261 ctggcgcggc cagtaccaca tgttctgtca gtacaacccc aatgcggcag tctggggcga
     4321 tatgcactgg gctcacgcgg tcagccccga catgctccac tggcgccacc tccctatcgc
     4381 gctcgcgccc cagccgcacg gcgccgatgc cgccggctgc ttcactggca cagcaatacg
     4441 cgacggcaac cgcgtcgttg tcctctacac cggcgtcatc gacaccgcgc cgccaggcag
     4501 ttctccgggc ttcaaagaat cgcaatgcat cgccatcgct tccgatcctg atctcacaca
     4561 atggaaccag gatcccgacc ccgtcatcgc cgcgccgcct cctgggctcg acgtcactgg
     4621 ctttcgcgac ccgaccccgt ggcgcgaggg caacacctgg tacctcgcgg tgggttccgg
     4681 catgcgcggc cagggcggag ccgtgctcct ctatcgttcc gccgacctgc gccactggga
     4741 atacctgcac ctcctcgccc acggcgtacc caccagtagc tccgaccatg atcctgtcgc
     4801 ctccggcgat atgtgggagt gcccggactt cttccctctc ggcgacaagc acgtgctcat
     4861 ctactcaacg cagggcaaga ccttttggca gtccggcacg ctcgataccg ccgcgatgct
     4921 cttccattcc gagcgcagcg gcattctcga ctacggctcc ttctatgcgc ccaagacgca
     4981 gctcgatcgc ctcggccgcc gcatcctctg gggctggatc ccagaaaccc gccccgtcgc
     5041 ggaataccgt gcagccggtt gggccggact gatgtctctc ccccgcgtgc tcacgctcga
     5101 taacaacaac gatctgcgcg tcgcgcttct gccggaattc gaccagctgc gcagaccgga
     5161 aagacgcctc cgtctgacgg gggacgcagc gcaggccggg cagcaacttg ccggcttgac
     5221 acttcgcgaa gcctgcggcg aaattgtcat ctctttccgt gaagagtccg agcctctcca
     5281 gttcgcgctc gtcgcagcgc agaatcctgc gaccagctgg ctcacttgta gctatgaccc
     5341 ttcttccccg ggccacatct ccatcgacga ccatcccttg ccgctcggcg ccggcgactc
     5401 cgccgccagc gtcgagttgc gtttcgttat ggacggctcc gtcatcgagt gcttcgccaa
     5461 taacagcagc accctcacga agcgcttcta ctatccaggc ccaatggcac ccgcaatcgg
     5521 catcgacttg aaaagctcga taaagaatct cactcgcctc tcgatcgcac aggtcgcacc
     5581 tatttcagcc aaccgcctga ccacctgaac ttcccagcga acacaacttg gctattggct
     5641 actggctatt cctaatcagt cgtcatcgtc cttccgcacc ccgtgcggat gcgtcagcat
     5701 ccacggcact ggcttcgacc ccatcgcgtc cttccagagg atcacgttca gcttcaccgg
     5761 atcggcccga tcctcgcgcg tgaagtccat cttcgccgac tcctttgccc cctcggcctt
     5821 cggcgcgttc gctgtgtaga tcagcccgtt gtcccggttc ctgtagtccg cgctgaacgc
     5881 cggctggtcc ccggccccgg aaaactccgg cgccatcagc ggcgcaaacg cgtcgttgtt
     5941 attcatcgga ggacacccca gcagcgtcag catcgtgtgc accgtgctca ccgtcgtgta
     6001 gaacgtgtga tccaccaccg gctcctcctt ccgtggtgcg tatttgctga tcacaagcga
     6061 aatcgaccga tgcgcatcca cgtgatccgc cccgtcctgc gcgtcatctt ccagcacgaa
     6121 gaatgctgtc gaatcccagt acgcgctgtg gctcaccgca tccaccgcgc gccccaccgc
     6181 cagatcgtta tccgccaccg atgctttagg cgtcggcatg cccgcacgcg tgcccgccgt
     6241 atggtcgttc ggcaaccgca gcatcacgaa cgccggcatc gtgtcgtgtc ccgccttcag
     6301 atctgttgtc cactccttga aatgggtcag aaactcgttc accctcagct gatccggaaa
     6361 gctcagattg aaatccggat actccggatc gaaatgtccc accagctccg gcttgaccgc
     6421 cacgttcttg tagatcagcg gaatcgccca cggatattcg ctcgttcccc cgccatagtt
     6481 tgccgggatc ggatcgcctt tgcggataaa cgcgtgctcg caatagtgct ctccctcagg
     6541 cgttccgccc gcctgcggca tcggccgatc cttgggcgcc tcgcccgaat cgtcacagaa
     6601 tttcgtcgag atgaactcgc cgaagtgata cagcgacttg tggctgcgag ccagatcggt
     6661 ccacagatac ccgctcccgg gctcatcgat gtccggaata ttctcaagca acggatagcc
     6721 gtcctccacc acgccctcaa agtcgtacgg ccgctcatgc ccgcgatacg cgatctgcca
     6781 tgtgcgctcg ttgtaatcgc tgttaatcgc cgccgtcgac cacacatgcc cgtcgcccga
     6841 cacctcgccc gagtcgtaga agttatccag caccccgaac tgcttcgcca gcgcatgcag
     6901 attcggcgta atgtcctcgc cgtacatcgt cagcgatgga tcgtcatccc ccgcgcccag
     6961 atcgccgaag atctgatcgt aagtccggtt ctccttgatg atgtagatca cgtgcttgat
     7021 tttgtcgccg cccccctcga acccgatctt cttctgtgcc gcattcatta ggttgctcgc
     7081 cagcacttcc ttcgtgagtt ccggcagatg tgcctttact tcgttcacat cgatcgttgc
     7141 cagcgacccg tgcagcatcg atgcaatgta ggtgtgcgcc cgcccttcca gcctcatcgg
     7201 cgcattggcc ggcggattcg gtgctgccgg ctgtggcgcc ccgttcggtg cggttccctt
     7261 gcccttgccc gtcgcaacat aaaggtgatc accctgggtc gcgattgccg tcggatacca
     7321 ctcggtcgga atgaacccgc tcgggatcgc gtgggcgtct ttcccccccg ccttcagatt
     7381 gaacaccgcc accgcgtccg accccgaatt cgctgcgtag agggtggcgc cgtcatcgga
     7441 aatcgcgacc ccatccggca tcgctccaaa gaaagcctgc cccggcagcc gcgtgtcgta
     7501 gagcgtcacc agccgcatcg tctgtcccga cacttgtacc gccgcgaccg cgtcgcggtt
     7561 cgccagcgcg acgtacagca tcttctcgtc cgggctcagc gtcatcgcca tcggatgcga
     7621 gctcggcgaa accggccgtg acggcgccag cagcggcaac tgctgcacga ccttgccgct
     7681 cttcagatcc agttccgcca ctgccgatcc attccacagc gacgcccacg cccgccgccc
     7741 gtctttgctc gccaccacgg ccaccggata tgtcgatggc acgaccggcc cgctcgacag
     7801 gtcgaaccgc gtctccagct ttccgctcgc cgtgtccagc aacagcacgt cgtcggagta
     7861 ctcgtccgct accagaatct tctccgcgcc gtcttcgccc ttcaccaccg caatccccgc
     7921 gggtgccgga atcgccttat cttcgcccag gtccgtcggc ccgtgatttt gcttctggcc
     7981 tccggtcaac tcgcgcatcg gcactggaat cagccgctcc ggcttcagca catccgttgc
     8041 ggacgcctcc ggcgcaaacc catagacgcc gatcgcgttc ccggtctcat cgggcttgcc
     8101 gccctccggt tcgctcagcg agtcgaagct cacatacaaa tgcgacccat cggccgaaaa
     8161 tgcgatcccg gagtaatagg tctgcggctg ctggacctcc gtccgcggct ccggataatc
     8221 cgtcagtttg cccgtcgtcg tgtccagaat cgcaatcgac tgctcgtagt tcgactccac
     8281 cgtgccgaac cccgcattga ccagcgccag atagcgatgg tccggagacc aggccgtggc
     8341 catcggcagg ctgttcagcg gctggggcga cccaggcacc gggcccacga tctccttgct
     8401 ggtcgacagg tggatggtct gggcggcggc aagtcccgcc accatcgaaa tcagcaaggc
     8461 gcaggtgata cgcagcatgc gaggtgtcat tcggttctat cgtctccagg gcggatgaac
     8521 gcggcgtaaa tgctacatca tccgagcgat ccagggctgg ccgggctcag gtaacacgat
     8581 cgtaacgacg attccgcccc ggtccaggga gccttacaaa agtgttacat ccgccccgac
     8641 aatccggctg ctacggtgga atgagcggcg cattttcgac gccgaaaatc catctccacg
     8701 ggactccaag ccttacatgt caactcaggt tgcaatcccc gaagcagagg aacagcttat
     8761 ctctgcggtt cctgttgcag gaaatgcggc tgccggtttc gcccggcagg aacgcatcgc
     8821 cgccatgccc aagctcggcc gtaagcacca gagcggaatg aactggttca ccacggtttt
     8881 catggtgatc ttccacgcgg gagctatcgc ggcgctgttc atgttcacat ggaaagccct
     8941 catctgcgcg ctcgtgctct gggtcctggc cactaacgtc ggcatcggca tgtcctacca
     9001 tcgcctgctc acgcatcgcg gctaccgcgt gcccaagtgg ctggaatacg ccctcgccgt
     9061 ctgcggcact ctcgctcttg aaggcggacc ggtcttctgg gtctctctcc accgcgttca
     9121 tcatcagctt tccgaccacg aaggcgaccc gcacaccccg cacgaaggcg gctggtgggc
     9181 gcacgccggc tggatcctca tcggcgactc ccttagcggc gaaaccgaat ccctcgcccg
     9241 ttacgtgccc gacctcatgc gcgatcgttt catggcgctg ctcagcaaat accactgggt
     9301 tcctcaggtg atcgccgcgt tcgtctgttt cgccatcggc ggatggccgg ccgtaatgtg
     9361 gggcatcttc ctgcgcacca ccatcggcct ccacgccacc tggctggtca actctgccac
     9421 ccacatgtgg ggctcgcgcc gcttcaaaac ccgcgacgac tcgcgcaaca actggtgggt
     9481 cgccctcatc accggcggcg aaggctggca caataaccac cacgcccatc cggtttccgc
     9541 gcgccacggc ctccgctggt acgagttcga catcaattac tacggcatct ggctgctgga
     9601 aaaggtcggc cttgcccgcc agatccaggt cgcccagtac gacccggcga atccgcgtcc
     9661 cgccggcacg gcatagaccc gcccctccgg cacccggaac ctgagaaaat gaaagtgctc
     9721 gcgatgcaag ccagtcgcgg gcactttgtc tttggtccta tgcgcctctc cggccgccgc
     9781 cgcaacatgg ttctcttcac cctgcttggg gtgtgcctca tcggcgttgc cgtcgtcctc
     9841 aacgtcggct ggatcgtcct caactggcgg cgcatcgtcc ccatggccat cggcatcccg
     9901 ttcttcgcgc ttcttatcgc cggcgtcgcc ctcaataccg tcttcctcgt ccgcgaagtc
     9961 cgccgcaacg agcgccacga tgccttcctg agcgccgtta cccacgagct caagaccccc
    10021 atcgcgtcca tccaccttta tctcgagact ctccagcgac gtcacatcga cgatgcccag
    10081 cgcgctgagt tctacagccg catgcttgcc gacagtgaac ggcttctcgc caccgtcgag
    10141 caggttctta aggccggcga agtcggacaa cgcgctcgat ccgatatccg tgtttccgtc
    10201 gacatgcacg ctctggtcga ggaatgcatc cgcaccacgc tccagcgcca ccatctcgaa
    10261 gagaaatcca tcggcctgcg cgtggacgaa gcgcagcttc ccatgttcgt ccgcggcgat
    10321 cccaacgagc tccagacggc ccttctcaac gtcctcggca acgccgtgaa gtactcgccc
    10381 gatggcgcga acatccgcgt cgcgctcagt atcgaaaacg atgcctgggt gcgtgtcagt
    10441 gtcaccgacc agggcatcgg cattcccgcc gcccatctca agcgcatctt ccgccgtttc
    10501 taccgcgttc ccactcgcac cgtgctcggc accaagggaa ccggcctggg gctctacctc
    10561 gtccgcacca tcgcccgcca gcacggcggg gatgccaccg ccgagagcga aggcgaaggc
    10621 cgcggagcca ccgtcagcct ccagctgcca cgcattctca ctccgctcgt ggggcagcaa
    10681 taaacgatgg cgcgcatcct gatcgtcgaa gacgaaacca atctcgccca gggtcttctc
    10741 tttaacctgc aggccgaagg tcatcaggtc agcgttgaga gcgatggcga atcggccctc
    10801 aattgcctcc gccgcgaacc cttcgacgcc gttgtcctcg acgtgatgct gcccggcaag
    10861 aatggatttg aagtggccac cgcgcttcgc gctgaaggca actacgtccc tgtccttatg
    10921 ctgaccgcgc gcgcccgcag ccaggacgtc gttagcggat ttgcggccgg cgccgatgac
    10981 tacctgccca agcctttcga cctcaacgtc ctgcttgcgc ggctgaacgc gctcctgcgc
    11041 cgcatgcaat ggcctcgcgc gccggaaaag cgacctgctc ggcccgacga cccttccgcc
    11101 ttcgactttg acggccgcca catcgacttc gaagccctgg agttgcatgc ccacggtcgt
    11161 accgtccgcc tcaccctcat ggagtccgat ctcctccgcc accttacgcg caaccgcgag
    11221 cgcatcgttt cgcgcagtga gctgctggaa caggtgtggc acgtgcgcga ggacaccgac
    11281 acccgcgcca tcgacaactt catcgtccgc ctccgccgct atatcgaaga tgaccccgcc
    11341 gcgcccaaat atcttcagac ggttcgtggc gtcggctacc gatttgtacc gaacccggag
    11401 caggctcatg attgatgccg gctctcgctc cgcatccatc gcgcggcaac cgcatctcct
    11461 aaaagggagg aaatgatgaa agaagtgacg ctccgcgtcg aaaacatgca ttgcggagcc
    11521 tgcgtgcgcc gcgtgtcgca ggcactggag aaagtcggcg gagtgaccgt cgatgaggtt
    11581 cgcatcggcg ccgcccgcgt ccgtgcgtcc gatgacgtgc cggagacgtc tattctgagc
    11641 gccatcgaaa aagcgggcta tccagctcac ctggagcact aaggagcctt gactgacctc
    11701 ggccttaaac gtcacggctt cggccgtgac ccaaagggga cttaagatat gcggctttag
    11761 cctctgaagg gtgttttcat tcctcagagt tctcgcgggg cttcaaccag ctatgcacgc
    11821 agccgaaaaa gcaaagaccg aaacaacacc cgcgatcccg gccaacggaa acgtccgcac
    11881 catcaccctc cccgtcatcg gcatgtcctg tgccgcctgt cagtcgcatg tcgaacgcgc
    11941 gctgcgctcc acctccggtg tcaccgacgc caccgtcaac ctgctcacca attcagcccg
    12001 cgtcagtttc aatcccgcca tcgcgaagcc cgaggacctc atcgcctcgg tacgggaatc
    12061 cggctacgat gcgaccttgc cggtcgaccc caactccgcg gcccccgccg aaaccgctgc
    12121 cgcagaaggc cccctccgcg ccaaagccat tttcaccctc gccggcggcg tgctcgtcat
    12181 gctgctcatg ggcgcgcatc actggttcgc gccggcggcg ccgctgctgt ttgcgatttc
    12241 gccccgcacc ctcgaataca tcatgctcgc catcaccctg gccggcatga tctggggcgg
    12301 cggcatcatc taccgacaag cctggaccgc cgccatccac ggtggcacca acatgaatac
    12361 cctggtcgcc ctcggcaccg gcgccgcgct cgcgtattcg ctggcagcaa ccaccatgcc
    12421 cggcctcttt cgccgtcatg gccttcaccc cgacgtctac tacgacgccg tcctcctcat
    12481 cctcggcttt ctcctcctcg gccgctggct cgatgcgcgc gccaaacggc gcaccctcga
    12541 cgcgctgcac gagttcatga agttgcagcc tcggtcagca cgcatcctgc gtaacggcca
    12601 gcaaatcgaa gtacccctcg ccgaagttgc cgctggtgac accgtcgttc tccgccccgg
    12661 cgagcgcgtt cccgtcgacg gtgtggtcct ctccggcgtc acctcgatcg acgaatccct
    12721 gatgaccggc gaatccctgc cggtcacgcg caagcccgga gatcgcgtca ttggcggctc
    12781 gctcaacttc gacggcgcca tcgagtaccg tgccacttcc gtgggcgccg acagcgttct
    12841 cggccagatg atgcggctcg tcgaagaagc gcagtcctcg cgcgcgccca tgcagcagct
    12901 cgccgatcgc gtcagcgcca ttttcgttcc aatcgtcctc gcccttgccg cggccacttt
    12961 cctcatttgg atcttcgcga gcggtggttc ggggcgagcc tttgccgtcg ccgtggctgt
    13021 gctcgtcatc gcctgcccct gtgccatggg cctcgccgtg cctgcagccc ttaccgtggc
    13081 gatcgggcgc ggagcccaac tcggcattct cttcaaaggt ggtgaaagcc tcgagcgcct
    13141 cgcccgcatc gacaccctcg tgctcgataa gaccggcacc ctcaccgaag gaaagcccga
    13201 ggtcgtcgcg gtccaggctc tcaacggcac atcggaagac gaagtccttt cccttgctgc
    13261 ggccctcgaa caaagatccg agcatcctct cgcccacgca attcttgacg ccgccgcccg
    13321 gcgcagcctc aaaattcccg cagccgatga agtccgcgcg ctccccggca agggcattca
    13381 aggcttcatc agcggtcagc gcatcgcagc aggaaactcc gttcttctcg acgatctcgg
    13441 tgtccccatc ccgaccgcta actccgccga acccggcacc acgacgatgt atgttgctcg
    13501 cggcagctca gtcgtcggca tcattcaggc tcgtgacacc ctgcgcgcaa cagcatccga
    13561 cgccatcgca ggtctgcgcc gcctcgggat ccgcaccgcc atgctcaccg gcgacacatc
    13621 cgcaacagcc gaatccattg cccgtgccgc gggcatcgat caggtctggg ccggccttct
    13681 tcccgatcaa aagctcgcca aaattcgcga gctccaaagc cagggccatc gcgttgccat
    13741 ggccggcgac ggcatcaacg acgccgcagc catctcccag gccgacgccg gcctcgccat
    13801 gggcaccgga gccgacctcg cccgtgaagc cggcgatgcc atcctccttc acggcgagcc
    13861 ccgccagatt ctcgacgccg ttctgctcgc gcgccaggcc ttgcgcgtta tgcgccagaa
    13921 cctcggctgg gcctttggat ataacctgct cggcatcccc atcgccgccg gcgtgctcta
    13981 tcccgccttc ggcatcctgc tcagccccgc catcgcaagc gccgccatgg ccctcagctc
    14041 ggtcagcgtc ctcaccaaca gcctgcggct ccgcagattt accccgcgat aaactgactt
    14101 cgtgcgcatc ctcacccgct acatcctgaa ggaagtcgcg tctcacgcgg ccatcggggg
    14161 tgccatcttt acctttgttc tcttcatccg ctatctcccg cacctgctgg agatgatcgt
    14221 ccgcaacagc gcctccctcg gctccgtctt cgaaatcgtt gccctcaccc ttcctgacat
    14281 gtgcaccgtc accatcccca tggcggtgct tgtcggcatc ctgctcggcc tcagccgcct
    14341 tgccgccgac agtgaaatca ccgccatgcg cgcctcgggc atcggcgtgt ggagcttcgt
    14401 gcgcgtggtt ggcgtcgtat ccgttgtcgc ctggggtgtg agcctcttca acactctcta
    14461 tctcgcgcca aaatcagccg ctgccatgcg ccgcctcgag gaatccctcg ccaacgcaca
    14521 ggcctccttt gaagtccagc cgcgcgtctt ctatgaggat ttcagggact acgtccttta
    14581 tgtgcaggac gttcggtccg gaaccggtgc ggccaactgg cgcggcatct ttctcgccga
    14641 tgtcacgcac ccgaatgctc caaaaatcac cactgcggat cgcgccaccg tcgtcaatag
    14701 ccgaaatcag gccatgacca tgcgtctccg cgacggcact gaaagtcagg ttgtccccga
    14761 tcagcccagc cagtacacca tttccacctt cggccagacg gatttgccgc tccagaccgg
    14821 aggtcaggaa gatgtccgcc tcggccgcag cgatacgccc atccttgcca tgagtacccg
    14881 cgagttgctc acccggtcgc atggccgcaa cggcagcatc taccaaatcg agttcgaaaa
    14941 gcgcctcgcc tatcctgccg cctgtatcgt tctcatgctc gtgggcgtgc ccctcggcat
    15001 ctcctcgcgc cgcggtggca agggcgcggg tttcgtcctc accatcgctc ttgtcttcgt
    15061 ctactacttc ctctcttcga ccgggaccgc gctcgcccgg cagcataagg ttccggcctt
    15121 cctcggtgtc tggcaggcca acattgtctt tgccgtttgc ggcgttgtat tgctgcgcca
    15181 gatggctacc ggcggcgcgg cgctctccgc cctcacctcg ctcggcaact ggtttcggct
    15241 gcgtgccgaa gagtccgcct cgtcggctgg cccggtgaag gccaccgtcc gtaaacgcgc
    15301 accccgcggc cgcttcccgc tcattttcga cgagtacgtc cttcgcgaat tcctcaccac
    15361 cttcgtgatg gtcctcgtca ccttcgtcat gctcatgctg atcttcactt tctttgagct
    15421 gctcagcgac atcatccgca acaaaacccc tctgatcacc gtaggggcgt acctcatcaa
    15481 tctcacgccc agcatgattt acctcatcac ccccctcagc gtcctcatcg gcgtgctggt
    15541 tgtgtttggg gtcatgaacc gcaacagcga gatgaccgcc atgaaggcca cgggcgtcag
    15601 cctctaccgg atcacggtcc cggtcgtcgc catcgctgcc atcctctccg tctcgctctt
    15661 catgttcgac gaactgtacc tgcctcaggc caaccgccgc caggaatcgc tgcgcaacgt
    15721 cattaaaggc aagcccgccg aaaccttcca gcaccccggc cgcaaatgga tcttcggtca
    15781 gcagcgtcct ggccagcccg gccgcatctt ttattacgag tacttcgatc cggatctgga
    15841 taccttcggc aacatttccg tttttgaatt cgctcctgga tccttcacca tctccaggcg
    15901 catttttgcc tcgagggccc attgggagcc cagcctgaat acctgggtct ttgaagacgg
    15961 ctgggatcgc accttcagcg gagccgagat cacctcctac gagtctttcc ccgtcagaac
    16021 ctttccggaa atctcggagg agccgtccta tttcaagaaa gaagtccgcc catcgtctga
    16081 aatgagcttc ggcgaactgg acgactatat ccacgacctg cgccagagcg gattcaacac
    16141 tgtcccgctg cgcgtgcagc tcaaccacaa gctcgcttat cccctcatca ccctggtcat
    16201 ggccgtgctc gccattccct tcgcgctctc gatgggcaag cgtggctccc tgtccagcat
    16261 cgccattgcc atcggcgttg ccattgccta tttcgtggtt tcaggattct ttgaggccat
    16321 gggcaatgtg agctggctgc ccgcgtttct ggccgcctgg tcccccgacc tcctcttcgg
    16381 cctcgccgga gcctacctcc tgctccgtac tcccacgtaa gctggaacaa tacgtatgtt
    16441 cagaaacctt cagcccctcc ttccttacat gcgccgctat tggcgcggct acctgtgggg
    16501 ctgtctctgc accgtcctcg cgaacgccat ctgggtgcag ttcccgcgcg tgatcggcat
    16561 cgccgttgac gacctgaccg tccgcgtgac ctggaacaag atcttcagct atgcgggcat
    16621 gctcctggcc gtcaccgtgg ctcacggcat cctgctcttc ctcatgcgct ggatcatcat
    16681 cggtatctca cgggatatcg agttcgatct ccgcaacgac ctcttcgctc agctggaaaa
    16741 gcagtcagcc tcgtactatc acgaccatcg caccggcgac atcatggccc gcatgacgaa
    16801 cgacctcaac gcggttcgca tgctgctggg tccggccatc atgtatacgg ccaacacggt
    16861 gctgttcttt gccggcgcgc tttacttcct cctccgcatc agccccatgc tcacgctgat
    16921 ggccctcgcc cccatgccgc tggccagcat tcttatccag ttcatgggcc gccgcattca
    16981 cgaccgcttc gagcgcattc aggccatgtt ctccgacatc tctgcccagg cccaggaaaa
    17041 cttctccggt gcgcgcctcg tccgtgcctt cgctcaggag gaagcccagg tcaaagcctt
    17101 cgagcgctcc aatcaggaat acatccgccg cgcgctccgt ctggttcgcc tcatggggat
    17161 gctctggccc acgctcgaat tcatgctggg ccttgccatg gtcatctcgc ttctcgtcgg
    17221 cggccatctg gtgctccagc atcgcattcc ggtcggcgag tttgtcgcgt ttaccaccta
    17281 catgctcatg ctcacctggc ccatcattgc tctgggctac gtggtcaatc tcttcgaacg
    17341 cggcaccgcg tccgtggtcc gtatccatga gctgctcacg gcgaagccca ccatcgatga
    17401 tcgggacgcc gtcctctcat ccgaaggctc ggaaattcac ggcgcaatcg agtttcgtga
    17461 cctgcacttc gcttaccccg catcgaacgg cgatccggtg gaagtcctgc acggcatctc
    17521 cctgcggatc cccgccggaa gcagcctcgc actggtcggc ccgaccggtt ccggcaaatc
    17581 cacgctggtc agcctcattc ctcgtctcta cgatgcgcct ccgggaagcg tgctcattga
    17641 tggacaaccg atccgcgcct ggcgcctcga ttccctgcga gccgccatcg ggtttgttcc
    17701 ccaggaaaca ttcctgttca gcaacaccat ccacgagaac attgcattcg gcgttcccgg
    17761 cgcttccgtt gcggctgtcg ccgaagccgc tcagagcgcc catatccgcg acgaatttga
    17821 ccttttcccc aaccggttcg aaaccacggt cggcgaacgc ggactcaccc tctccggcgg
    17881 gcagaagcag cgtaccgctc tggcccgggc gattctccgc aatccgcgca tcctcatcct
    17941 cgacgatgcc ctggccagcg ttgacaccta taccgaagag cagatcctcg aagagctccg
    18001 cagcgttatg gcggggcgta ccaccatcct catctcccac cgcatttcaa ccgtgcgcca
    18061 tgccgaccgc atcggcgtcc tggtgcgcgg ccagatcgtg gagctgggca cccacgagga
    18121 actgctcgcg ctgaacggct actatgccag cctgttccag aagcagctgc tcgaagaaga
    18181 gctggccgtt acacactaat ctccctaagc agtccagctc gaagcggccg gttcatcaaa
    18241 acccgccgac agatgcgtgg ccgctgtggc caccatctgc ggctctgcat cctctccgat
    18301 caggcgcaac acctcaggat ctcgcgccgc caccgcccac gcctcgcgca catcgcgaat
    18361 gcacccgatc gcttcccgga agcggtccgc cgactccagc cgcgatccct ccagacacaa
    18421 cgcaaaaacc gccgcataga actcgctcag taccttcgcc gagtctccgc ctacgtcggg
    18481 acgcatccgc gactgcagat gcatcaggat ctccagcacc ctcccgatcg ccgtccgccg
    18541 cgccgcggtg tcgcggcgtt ccaccgcatc gatggcgttg cggagaaaac gcatcatccc
    18601 gtcatacagc gcaacgatca gttccaccgg attcatcccg gcaatggcct gctgctgata
    18661 gttcatgcct ctccgcctgc gtctgcctct cagcgattac cgttgctggt tgtaaccggt
    18721 aatcgcgctg taaatctggt ttacatactc gatctgttgc ggaatctcct gcaggatgaa
    18781 gttagccgca ttcaattccg tcgtcagttg cttcttctgc gcgtcgatca gctcttcctg
    18841 attgctgatg ttctggttca gagccttctc ctgcgccgag ttctgctgca gcgccagata
    18901 gattgcaccg tgaggaccac tactgcccgc gttgttcagt gctgtcgtca gatttttccc
    18961 gaacgatgtg taacttccgc tcggctgaaa gaagttcatc acgtcctggt agctgctgtt
    19021 gagcatggtg tccagcgtcg ccgtattcag cgtcagcgtt ccgtcatact tcgtgctcgg
    19081 ggtgatgccc agctgcgaaa gcgctgtcac cgccccggaa gactggacga acccaatcgc
    19141 ctgctgcagc tgctcctgca gcatcgcgac tgtcggatta ccgtagagcg gctccgccgt
    19201 ccccgaggag ctgttcgcct cctgggtgtt cagatcgccg atgaccttgt tgtacgcatt
    19261 cacgaacgcg ttgatatccg attccacctg actgttgttg ttcgtgattt caacctgcac
    19321 cgaattgccg ggcggcgcgc tcagcagctg catcgtcacg cccgggatcg ccgtcgtgac
    19381 cgtgttcgag ctgctgctca tcgcgggcag tccatccacc gaaaactgag cgtcctggcc
    19441 cttctgctgc cggctgaagc ttacccctcc cgccgtactc gaaccggtgg tcgcatcgct
    19501 gagcccgctc gcatcgaggg tcactgagcc cgccgccccg ctggtcgtgc tgatcagggt
    19561 gagctccgat tgcccggtgc ccaccggcac gacgttcgcc tgtacgccgt attgtcctgc
    19621 attgataaaa gaggccagcg tcgccagagt gttgttctgc gggatcagcg caccgctgct
    19681 cgggtccctg accgttccgt cggacaggga gatggtctgc gagccgatct tcaaagtgcc
    19741 actgagcgtg tcgccgctgt taaccgccgt gctcgcgtac gaaaatgcct tcgccagctg
    19801 gctgaccacg atggtgtggc ttccggccgc cgccgccgac gtcgcattca tcagggccag
    19861 caccgagttg tccgagctcg atccctcttt gcccgaaaat gtcccctgga aatcggtcag
    19921 cgtctgcagc gccgttgcca gcgagctgag atcggtcccc atgctcgtca gcgccgtgtc
    19981 ctgagccccc agcgtcgtca actggttctt ccacggcgtc tccaccgcct gcagattcgt
    20041 cacaatctta tccaccgtgg ccgagacatc gaagccctcg ccgctggtgg gcgaaccaaa
    20101 cgacagaccg acgcttccca tgctgctctt atcgacgctt tccggataaa ccttatccag
    20161 cgcctgaaac ctgcaggtgc ttcgccgtgt tggggggcag cgatcttaga aggccctaag
    20221 acagcgcggg gagccgaagc tccccgcccg ccgttcgctg ccggtttgtt tactgcagca
    20281 gcttggtcac catctgttgt gtgctgttgg cctgcgccag agccgagatg ccggtctggg
    20341 tcaggatttc gtacttcgac atgttggaag tcgccgaagc atagtcggtc gccgtgatcg
    20401 cgttctgcgc cgacgtgatg tttgtctgct cggaacttgc gatgttcgac gccgctgtca
    20461 gcgtgttgat gttcgcaccc acctggccgc gctggtaggc tacggcgctg atggcgttgg
    20521 ttacggtttg cagagccgct tccgcattgg cctgcgaggc aaggctggtt cccgacaggc
    20581 tggtgccgac cgtggcgccg gctgaggtcc cgccggtccc ggtcaccccg cccaccgtcg
    20641 ccgtgatctg cgttccctgc acggagttca gagccgagga cccaacgctg gactgagaca
    20701 gggccccaat cgtcacgttg tagttcttgc tgcccgcgct ggtgccatca ctggtgtaga
    20761 tgttcacgcc gttggtcgac tgcgagagct gctcgatccc gttggtcgac gcatttgcga
    20821 tcgtgaagcc ggtgattccg gccgccacgc cagccgcgga tacgccgatg gtcaggttgt
    20881 cgttcagcgg cgcaggggcc ccgttgttgt tctggttggg attaggctgg ttgaccggag
    20941 tcaggcccac cagataattg ctgcccaggg ctgcctgcag agccgacacg aggtcggtgt
    21001 tgtccgtaga ttctccagca aggttgacga caatcggcgt gctgctcgag ccggtcggtg
    21061 tcagcgtgaa gccgcccatc accgcggagc ccgtgatgtt cgcctcggtg atctgcgact
    21121 gcgacagcgc attgctgccc gtcccgttgg tgatcgcgat ggagtcaacg ttggcgttga
    21181 tgccgacctg gttgatggcc atgctcaccg ttgtgttctg gttcacggta ttggcgctca
    21241 ccgtgtagta ctgcgcgttg tttccgagtg ccgtggtgat cgctgtgacg aggtctgcgg
    21301 cgttgtgggc cgtgttgatg ccgttgctca gattcagagt gatcgcactc tgcgcctgcc
    21361 cggcgatcgt ctggttgatg gtcagctggc cgcccagaac ctcattgctg gtgaaggtga
    21421 gcgatgcctg gttcgctgcc gtctgggtaa tgttgcccga cggagccgca ttccacgaaa
    21481 tgccggtaat gttgttcgcc gttgccgctg cggtgagccc cacgctcagc acaccggtac
    21541 cgctgttgta ggccacggtg taattcgggt tcgagacgtt gccgttgagc gcctggctga
    21601 ccacgctggc caggttggcc gtgttggtgc cgttcaggtc gatggtcgtc ggtgtcaggg
    21661 cggttgccgt cgccgaggtc gcctgctgca ggccgcttgc cgtgatggag gtgatgccgt
    21721 ggcccgtgcc gccggcagtc gttgcgatgg tcagcgtgcc gttggaatag cttacggtga
    21781 agtcgttgtg ccagtcgttg accgacatcc ccgactgcgc cgccagacct gccgcgatcg
    21841 tgtttgccag gttcccggtg gtcacgccgt tcaggttgat cgttgccgcc agcgtgcccg
    21901 tcgtgggatg ctgtgacgac gctccgtgcg ttgcgcttgt accggacacc gcgaaggacg
    21961 tcaggccgtc tgccggggtg ccgttcactg cgagcgtcag cgcgccggtg gtgtggttat
    22021 aagcaactgt gtagtcgccc gctgttccgc ccgcagcggt gatctgcgct ttgatcgcag
    22081 tctggaacgc ggtgtcgctg gccgcgtcat gaccggccgt caggccggtc agatcgacgt
    22141 cgagcgacgt tccgtccact gtaccgcccg tcaacgcgat cgtgatcccg cccgccagga
    22201 catcgccggc agcaatcgtg gttccgttgg ccgcccagac caggttcgag accgcgcccg
    22261 ctcccgttac cccggcattc gttgtcagag tgagcgtgcc cgacaacgcg tccgggttgc
    22321 cgttgacgct ggcaatggtt ccgctgacgg aagcggttcc ctggccgttc cagaccacat
    22381 tcgaagcggc tccggctgcg gtgttggtca gagcggttaa agtcagcgta ccgctcaggg
    22441 aatccccgtt ggcggcggtc agctgatagg tgccggcgct ctgcgcggct gccgctgagc
    22501 cggtgttgct gatgaccggg aagcctgtca ggtgatcggc cgacgcgtgc gccccgaatc
    22561 cgatttgcag ctggttgccg ttcacgacgg acacggtgta gtcggctccg cctgcctgcg
    22621 tattcatcgc cgtctgcagc tgcgaagccg cagtggtcag attggtcgag gtgatcgccg
    22681 tgcccaggtt cacgctgatc gcgttcgggc ttccgctgct ggccgtaggg gtaaaaccga
    22741 aggtgcccgt cagctgcgaa ccgttcacga tcggcccggt cgtgatcacg ctgtaagcgc
    22801 cagcgccgcc cgccgtccag gtgagtgccg tcggcgaagc cgcaaaggtc ccgccctgcg
    22861 tggcgttgac tcctgccgtc gtcccgccag ccacgatatt gccgcccgca agcgcagcgg
    22921 cggtcgccga ggccgacgtc gcggcggctg tattggtctg cgttccgccc aggactgaag
    22981 accacgtcag cgcgctggcc gtcttgtcgg cgccaaaaac ctggattccg ttgtattccg
    23041 tcgtgctgcc gatcgtattg atctggttca gaatgtcctg gtactcctga tcggccgcgc
    23101 tcatctgcga agccgtcagg gtgccgccgc ccgcttcggt ggccagggta atggcccgat
    23161 tcagcaggtt cgtcacctgc gagagggctc cgtcggccac ctgcaggaaa ccgacgccgg
    23221 ccgtcgcgtt cgctgaagac tgcgtcaaag ccgaagagtt cgcctgcagt ccgttggcaa
    23281 tggccagccc ggctgaatcg tccgcgccgg aattgatccg cgacccggag gacagctgtt
    23341 gcaatgtgtt ttgcaggttg gtctgcgtgg tgttgaggtt gttctccgcg tagatcgccg
    23401 agatgttgtt caggactcct aatggcatat cgtatctccc ttgttcaatc gggttcctga
    23461 acagggctcc ggacctttga cccttacacc cgcgggaacc gcatgactca tacccttcat
    23521 cggccccccc tctttgccct ttatcctcct tgctccattt ggcaatccgc gtgcaccctc
    23581 cgctgccgca tttctcccat tcatctcctc accccgcccg ccgataggag ataacaggcg
    23641 caaaggcgcc cgcccatgat ccctctcacc cgcctcaacg gtaattccct ggtcgtcaac
    23701 agcgacctca tcaagtgcgc cgagtcctcg ccggacacca tgctcaccct catcaacggc
    23761 gagaagatcg tcgtccttga gagctgcgac gaggtcgtcc gtcgaaccat cgcctggcgc
    23821 ggctcggttc tggcggaagc agccacgcat ctgcaggccg tgccctggac ctctacggcc
    23881 atcctgcccg gcgcggaaaa ctctgaggct tccgccacgg cctcttcgcc cgacactcgc
    23941 cgggcaaact ccgaggcctg agccatggac gctggctccc tcggcggtct tctcctcgct
    24001 ctggccggga tcctcgccgg ccttctcttt gaaggcggca acctcagtca gattctgcag
    24061 ccgaccgccg ccatgatcgt ctttggcggt acgctgggcg ccgtgatgct gcagtttccc
    24121 tttcgcatcg ttcgctccgc cgcccttggc ctcgtccgcg tcttcttcca tcgcggcgga
    24181 ggcgccgccg ccctcctccg caccatcgtt gccttcgcca acaaagcccg ccgcaagggt
    24241 atcgtttcgc tcgatcagga tctggctcag gttaccgatc cctttctgcg tcaggccctc
    24301 atgctggccg tcgatggcac cgaaccctcg gagctccgtc gcatcatgga agttcagatc
    24361 gatgccgtca gcgagaatga cgcgcgcatc cccgccgtct ttgaatccgc gggcggattc
    24421 tcccccacca tcggcatcat cggcgcggtc atcgggctca tccaggtcat gcagcacctc
    24481 gatcacatgg acgaggtcgg ccgcggcatc gccgtcgcct tcgtcgccac catctatggc
    24541 gtcggcgccg ccaatctttt ctttcttccc gccgccggca agctccgcca gcgcgcccgt
    24601 gaagatcagc gccttcgcga gatggccctg gaaggcgtta tctccatcgt cgaaggcatg
    24661 aatccgcgca tgatcgagat caagctcgcc ggctttcagg gcagcgctgc tgcgccatcg
    24721 gtcaacagcg atcagccgcg cctggtcgcg gagaagcgcg cttgaactcc ggccggctca
    24781 aatcccgtcg tacctccgac cacgttcacc atgaccgctg gctggtttcc tacgccgatt
    24841 tcatcactct gctcttcgcc ttcttcgtcg tcctctacgc cagttcgaag gctgaccagc
    24901 aaaagcaatc gcagattgcc cactccatcc gttccgcctt cgaagctctc ggcgtctttc
    24961 cctctccgcc tcgcgccgac agccacgcgc cacccgattc ccaggcctcc attcctcccg
    25021 tcaatgtcgt cctcggcgac gaccttgccg cttctcccga aatccagcag gatctgcatc
    25081 ggctccagaa cgagctcgcc gcgaccctcg cagcccaggt cgcccagcac aatatcgcgc
    25141 tcaaactcgg ccgcgacggc ctcacgatct ccctgcgcga atccggcttc tacgacagcg
    25201 gttccgccct cccgcactcc caatccagac ccacactcga aaaagtagcc gctaccatcg
    25261 gcgccacgcc ctacgacatc cgcatcgaag gccacaccga caacgtcccc attcacacgg
    25321 cgcagttcgc ctcaaactgg gagctctcca cggcccgcgc caccgaactg gccaagctgt
    25381 tcatcgtcaa ctaccgcttc tctcccgctc gcctctccgc tgccggcttc gccgaatatc
    25441 atcctgtcgc cggcaacgac accccggagg gccgcagcca gaaccgccgc gtcgacatca
    25501 tcgttctgcc cagaatcagc gtcgtcccga ctcagaacca acactaaatt cccgcatctc
    25561 gcaccagcgg atgactccct cgcttcttta ctcgctctta ctcgcttttc tctcgcttct
    25621 tcctcgcaac tcgctcgccg ttcgctcgtc ttctgcttcc tccacctgcg ctccgcgccc
    25681 tgccttcaaa atcccagcac cgggtgcggt acatacgccg cctccagcgc cgccatctcc
    25741 tccgacgtca gcttcacagc gagcgccgcc actgcgtcct ccagatgatt ccgcttcgtc
    25801 gcccccacaa tcggcgctgt aatccctggc ttcgccagca tccaccccag cgccagcgat
    25861 gcctgcgaga ctccgcgttt cttcgccagc gcagtcagtg cctccacgac tttctgatcg
    25921 gcctcttccg tcttcgagta catcgtgttc ccgaaccggt ccgtctccga ccgcttcgtc
    25981 gtctcgcttt gccacggccg cgccagccgc ccgcgcgcca acggactcca cggaatcacg
    26041 ccgatccctt cctcccggca cagcggaatc atctcccgct cctcttcccg atagagcagg
    26101 ttgtagtgat tctgcatcga cacgaaccgc gtccacctgt gcttctcagc cagatacaaa
    26161 gccttcgcaa attgccacga ctgcatcgac gaggccccaa tataccggac cttccccgcc
    26221 ttcaccacat cgtgcagcgc ctccagcgtc tcctcgatcg gcgtctcctc gtcccagcgg
    26281 tgaatctggt acagatccac gtagtccatc tgcaaccgct tcaggctctc gtccagttcg
    26341 aaaacaatct ccttgcgcga gagcccccgc ccgttcggct cttcgcgcat cacgccccac
    26401 accttcgtcg ccaccaccac cgactcgcgc cgcacgttct tcttcagaaa acgccccagc
    26461 acctcctcac tcgctccgct cgagtacaca ttcgccgtgt cgaagaaatt gattcccaga
    26521 tcgagcgcct gcttcaggaa gggctggctc tcctcttcgt tcagcgccca cgcatgccgc
    26581 cccgcctgcg gctcgcccgg tgccggtggc cgcccatagg tcatgcaccc cagacaaatc
    26641 cgcgaaacct tcagccccgt cctgcccaga ttcacgtatt ccatcgcaac tcccttccgg
    26701 ctgtggcgca ataagcgcat gcaaagagcg tacgaacatt cgccatcccg atgctaataa
    26761 cacagattcc cgaactccag cgctcgattc gcgcaacctg gccccctgcg ctctgagctc
    26821 tgtgccctgt gctctgcgct ctgagccctg taccctgtaa cccgtgtccc ccgcccgtcg
    26881 tctgcgcatc ctgtacgacc gcctcgtcga gacctggggc ccccaacaat ggtggcctgc
    26941 tcaaacccct ttcgaagtcg tcatcggcgc ttacctcacc cagaacaccg cctggaagtc
    27001 cgtcgagcgc tccatcgcca acctccgcag ccgccgcgtc ctttctgtta gcgggctccg
    27061 caacctcccg gaagccgaac tccgcgacct gatccgcccc tccggcttca tgctgcgcaa
    27121 agctgccgcc ctcaaagcgt tcatcgtctt cctcgatgcc caccacaacg gcagtatgcg
    27181 ggcactgcag cgtgcggctc tcctcgatgc aagcgctgtt cgcaagcagc ttctcgatct
    27241 tcccggcatc ggccccgaga ccgccgacgc cattctcctc tacgcgctca aggttcccgc
    27301 catggtcgtt gacgaatacc ttcgccgcgt tgccattcgc cacggcctcg tcccgcacca
    27361 tgcgaaatac gccgagattc agaccctcgc tgaacaagcc ttcgtccccg aacgctccag
    27421 cgaaaactcc gacgaactcg cccgtcatta caacgagttc catgctctga tcgttgagac
    27481 cggtaagctg cattgccgtc gcatcccgct ctgcgagggc tgcccgctcg ccttcgatct
    27541 gaaaaacccg ccattttcgt atcccgtgca taaaatgtac gggtgacggt gactctctac
    27601 gcggcgacca ccaatcctgg caagctgcgc gacttctccg tggctgctgc ggatgccgga
    27661 ttcctcgtcg agtctgcccc gcgcctgcgt gcaatccctg ctcccgaaga aaaagagctg
    27721 accttcgccg gcaatgcgag gctcaaggcg caatactaca gccgtctcct tcccgggttc
    27781 atcctggccg acgactccgg cctcgaagtc gatgcgctcg gtggcgaacc cggcgtgcgc
    27841 tccgcacgtt acgccgacga cgcgggcttc catccaaagg gctccggccc cacactctcg
    27901 accgacgaac gcaacaatct ctacctcctc tcccggatgc tgggcgtaac cgatcgccgc
    27961 gcgcgctatc gctgcgtcct cgccgtcgcc tgcgagggag agttgctgct caccggcgag
    28021 ggcacggtca gcggcgagat tcttgccgca ccgcgcggcg ccggcggctt cggctacgat
    28081 ccgctcttca acattcccca gctgctgctc accatggccg aaatttctct ggaacagaaa
    28141 cacaccctga gccaccgcgg acgcgccttt cgcgcccttc tgcccgggct gaaaaagctc
    28201 agcggctgac ctgcatgtcg gacatcattt tcggactccg agcggcgtcg gccaaacgga
    28261 tgcgctcttt ctgaacgctg aaaaccgaac gctgcctcga agccccacta caataaccgc
    28321 tgagtcatgc gcatcgacgc ccaccaccat ttctggcact acacacccga tgagtacggc
    28381 tggatcaacg acgccatgtc cgtcatccgc cgcgattttc tgccttccga tctgcaggca
    28441 gaaatctctg ccgccggtat cgacgccgtc atctccgtcc aggcctgcca cacgatcgcc
    28501 gaaaccgatt ggctcctcgc aaaggcagcc gcccatccct ggatcgccgg agtcgttggc
    28561 tggctcccgc ttgccgatcc tggaattggt gcgcttcttg agcgtttctc ggcgaaccca
    28621 tggttgaagg ccatccgtca cgtcctccag gccgagccgg atgcctatat ggaccgcccc
    28681 gacttcaaca acggcctcgc gcagctgcgt gccttgcctc tcacctacga catcctcatc
    28741 taccactccc agttgcccgc cgccatccgc ctcgtggacc gtcaccctga ccaggtcttc
    28801 gtcctcgacc acattgcgaa acccgcgatc cgcgccggcg aactacagcc ctgggcacaa
    28861 aacctcgccg aactcgcccg ccgccccaat gtattctgca agctctccgg cgtcgtcact
    28921 gaagcgaact accaaacctg gacgtacgac caaatccggc cgtatctcga agccgccctc
    28981 gaagccttca cgccgtcgcg cctgatgttc ggctccgact ggcctgtctg ttgtgtagct
    29041 gtgtcttatg tcgactgggt ccgcaccatc gagcgattca cgacggcgct ctccgccacc
    29101 gaacgcgaag ccatcttcaa cagcaccgca gcgagcgcct accggctgga ctaaaaccag
    29161 caggtcccag ccatcctggt cgctattggc tattggctat tggctactgg ctactggctg
    29221 ccttcaaatc tcaagcacag tgacaatttc cccggccgcg agacgctcca ccgtgtccgg
    29281 aaccaccaaa aacccgttcg ccctcgccgc tgccgccaga tcgcctgatc cctgccagcc
    29341 gatctgccgc acgtgaacac cgcggaagct gctcaccaca attgcaggga gaaaccgcgt
    29401 cagtccaggc ttcacgcgca cctctttctc cagccgcgcc agggtaaagc gcggccgctc
    29461 gtcgctctcg cctccgagtg cccttaacag cggcgctcca aacaacgtaa aggtcacgat
    29521 ggtggagatc ggatttcccg gcagtcccag gaaatgcgtg tccccgagca ttccaaacac
    29581 caccggcttg cccggctgga tcttcgcccc ggtaaagtag aattccgatc cgagccggac
    29641 cagggcctgc tccacgaaat cgtacttgcc catggaaacg ccgccggtga gcacgatgag
    29701 gtcgcagtcc cgggcgtcgc gaatggccct ttccgtcgct gccagatcgt cgtgcacagg
    29761 cggcaacacg atgggttcgc cccccgccgc ccgcacctgt gccgccaatg agtaactgtt
    29821 ggagttacga atctgatgcg gcgccggctt tctgtccagt tccaccagct catcgccggt
    29881 ggccagaatc gcgacccgcg gcctgccgaa taccgccagc tgtgcggcgc cgcatgcggc
    29941 tgccgcggcc acttctgcga tcccgatccg cccccccgcc gggaccacca cgtcgccggc
    30001 ccgtgcttcg gcgccggccg gaacgatgtt ttccccggcc gtcatttctc gtccttcggc
    30061 caggctgatg tgttcctcgc cggacacaac gtgttccacc atgaccacgc agtcggcccc
    30121 gggggggacg gcggctccgg tcatgatctc cacggcctcg cctggacgga tcgcgcccag
    30181 cgtccacgcc tctcccgcac gcagcaggcc gaccacgcgc agcccgcctg catccagatc
    30241 cgccaggcgg caggcatatc cgtcccgcgt tgcacgaggg aaaggcggct ggtcccgatc
    30301 ggcgacaaca ggttctgcaa gaacccggcc tgccgcctct cccagcggca ctttttcgac
    30361 attcggtttg cgcgatgctt cttcgcgagc gcggaccgcc tggcggtgca ccaccgaggc
    30421 cgcgtggtca tagctcagga cgatcgacgg cgattcctct ggcgtcacaa aacggtcctc
    30481 gcaaaaataa aacagcgccg tcccgttctg caggtccggc gctgctcgtc ttctttcctg
    30541 cttagtgttt cttcccgctg gacggcgtcg cgctcagctc ggccagtacc gatttcgcgt
    30601 ccgcggcaaa cgggccggtc ggggccagct caagatactt cttgtaagcc tcctggcatc
    30661 cctccggcgc cacaatcttc tgcgtctttg ggtccacggt tgctttattc agcagggctt
    30721 tccccttcag gtagtacgga atcggcttcg ttgcatccgc cgcgatcgcc ttgtctgccg
    30781 ccgcgacagt ggcgtcgatc tggttatttc gatccatgat gatggcttcg ttggtgtagt
    30841 acatcgtcgc ctgcggcgga ttcgccttca ccgcttcatc gtaagccgcc tgtgcatccg
    30901 gaatcttgtt atccgaacca taggcctcgc ccagcgcatt gccggctgcc gcgatgacct
    30961 cgttattcgg cttcttcgac gcctgctcca gttcgatccc cttcttcagg gatgtctcgg
    31021 cgtccccgta ttgcttcagc cccagctggg cttcgcccag ctcaacccaa agcagcggct
    31081 cgtccggttt tgccgtcacg tccttttgca tcagcgccgc cgcatcggaa aagttctttg
    31141 ccgcccgatc cgagcgcgac tttgccaggt ccgcgttcag gttcttgatc tgcgcgttct
    31201 ccttcaggat gctcgcattc ttcgacttcg cctcttccag cgccttgcgc tcttcggggc
    31261 tcagcttggc cagataatcc ggccgcgaca tatcgtcgtt cgcctcggtg tcctgcccag
    31321 cggtcacgtg gatgttgtcg atctggtccg ccaccttgtc cttcggcgtg ttcgcctgcc
    31381 gataggtcat cgtgtagctg cccggggcaa gctcgccctt aaagtcgccg ttcgcatcca
    31441 catcgaaggt gtacttggga gtggcgttcg ccgatgccaa accgccttca tacagcgaaa
    31501 ccgtcccccc cgtttcaggc gttcccgccg gatcgctcac gtgcccgtgg atcttgcccg
    31561 tcgttgtctg tgctcccagg aacagcggag tgctcaaaag aacggccagc gccacgcgcg
    31621 gcgcaacgtg tttcatcctc atgttcattc ctctctgctg gagtgcgcgg ttatgccgcg
    31681 ccctcctgac ttgtctctcc agcctgggct cccgtccatc cccgtgttgc gtaagggatt
    31741 gggtccttcg cccctgcctg ctcgaaagcg cgcagccgca gcacacaact ctcgcatacg
    31801 ccgcacgcga cctccgagcc cgaatagcac gaccacgtta actccagcgg cgcagccagt
    31861 tcaaggccga gcgccacgat ctgcttcttc tgcatatgga tgagcggcgt ccgcacaatg
    31921 atgtcgccct cccgcgttcc ctggtgaatc agctcctgaa acgccccata ataggccggc
    31981 cggcagtccg gatagcccga gctgtcctgc tccactgccc ctatcaacac cgtttttgcc
    32041 ccaaggactt ccgcccagct caccgccgcc gacagaaaat gggcgttccg aaagggaaca
    32101 tacgtgactg gaatctccgc gccaatcgtc gcgctgtctg tcggcgcctc aggcacttcg
    32161 atgcgctcgt cggtcagcgc ggacccgccg atcctccgga acagatccag tttcaaccgc
    32221 aggaaatccc ggaatcccag cgcatcggca actgcatggg cggcctgcgc ctcccgctcc
    32281 tcggtccgct gtccgtagct gcagtggagc gcataggcgt cgaagtcccg cgccgccagc
    32341 gccgcacaaa cgcacgagtc catcccgccg gagaggcaca ccaccgcgcg ttccctgtgt
    32401 gaggattcgt ccgcctggct cacccttcag cttaacccga tccgccgccc ggcgtgctgt
    32461 gacgcaccgc actgcggtcc ataggctatg ccgcctgagg ccgggagctc ctgtacgccc
    32521 agaccgccag ccccacaaac agcaccagcg cg
//
LOCUS       DASZYN010000448         2678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_662313, whole genome shotgun sequence.
ACCESSION   DASZYN010000448 DASZYN010000000
VERSION     DASZYN010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662313"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VHE33_11580"
     CDS             complement(<1..272)
                     /locus_tag="VHE33_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVT98137.1"
                     /translation="MPDAKLQIIPLGGLGEFGMNCLALRYKDDIIVIDAGLMFPESEL
                     LGVDIVVPDISYLVENKSLVRAIILTHGHEDHIGGLPWILSELNVPV"
     gene            448..1014
                     /locus_tag="VHE33_11585"
     CDS             448..1014
                     /locus_tag="VHE33_11585"
                     /inference="COORDINATES: protein motif:HMM:NF016542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opioid growth factor receptor-related protein"
                     /protein_id="HVT98138.1"
                     /translation="MAANEPERRNSSLTPSPANRLVAFYRDGAPDDEGRTLAEILAWD
                     DDRLEAIHDFIQWLFPLPEPSGANPLAPILDPATIRIFQSSIAMQDRLRQSFQRMMRF
                     YGFRQVTTPAGIAIERAADFSARAQNWLWPMNHNHLRLTRILRSTLLLGLAAESKALF
                     HALNALYREYPDRIPSRTHAFWSSAAGS"
     gene            1096..>2678
                     /locus_tag="VHE33_11590"
     CDS             1096..>2678
                     /locus_tag="VHE33_11590"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98139.1"
                     /translation="MLVRRSLLIFALLSAPLMLPAQAPNPIHGENGITLPAPPVAKVD
                     TVTDDYNGTKIEDPYRWLEDAHAPATRAWITEENNYTQKYLSQLTTLPKISSELTALM
                     RVDQYSIPTLRAGKYFFKKRLADQNQGSIYTRTGWHGKDELLIDASKLSADQNTSVNM
                     VDVSDDANLVVYEIRVGGADEGSVHIRSVKNGSNLADVLPTARYSGISLAPDNSGLYY
                     AKFTHEGTNVWFHKFGTPVHTDQKLFGGEYKGRQLGELDLISCDITDDHHYLIVSIAH
                     GVPATSQDYLVKDLRKTSADFVPLVYNVEAHVRVLEAADRFFLMTDYEAPNTRIAEAT
                     PGSEASAWKTIVPEGKEVIDSYSIVGGKLFVSRLHDVKTETTIYTLDGKQIGTLSYPG
                     IGTGTVVYGYPTQTDGFYTFDSFILPPTIYRYDTKTGRSDVFAAPKVPFDSSQYEVTQ
                     VFFNSKDGTRVPMFIAGKKGLPRDGSARLLMTGYGGFDLAELSTWNPEYAWWMEQGGW
                     FALPNLRGGNEYGESWHKAA"
BASE COUNT          548 a          997 c          653 g          480 t
ORIGIN      
        1 accggcacgt tcagctcgga gaggatccac ggcagcccgc cgatatggtc ctcatgccca
       61 tgggtcagga tgatggcccg caccagcgat ttgttctcga cgagatacga gatatccggc
      121 acgacaatgt cgacgcccag cagttccgac tccgggaaca tcagcccggc gtcgatcacg
      181 atgatgtcgt ctttgtagcg gagggcgagg cagttcatgc cgaattcgcc gagaccgcct
      241 aagggaatga tctgaagttt tgcgtcaggc atcggaggga cttgccgtcc aggcgttcgc
      301 gccttgcggc gcacgaggtt ggtgggagtg gaacgacgga aaagaatcat tcacaggata
      361 tcagcgaata cgcgtgtccg acgcgcccaa acacgctact ctaccaggga tggccgtcgg
      421 ttccgcgcaa tgccctcggt tgggcttgtg gcggcaaacg aaccggaaag gcgaaattcg
      481 tctctgaccc catcccctgc aaaccggctg gtcgcgttct atcgcgatgg tgcgcccgac
      541 gacgaaggcc gcacgctggc cgaaatcctc gcctgggatg acgaccgcct cgaggccatc
      601 cacgatttca tccagtggct gttccctctg cccgagccct ccggcgccaa tccccttgcg
      661 cccatcctcg atccggctac catccgcatc ttccagtcct ccatcgccat gcaggaccgc
      721 ctccgccagt ccttccagcg catgatgcgg ttctacggat tccgccaggt caccacgccg
      781 gccggcatcg ccatcgagcg cgccgccgat ttctccgcac gcgcccagaa ctggctctgg
      841 cccatgaacc acaaccacct ccgcctcacc cgcatcctgc gctccaccct gcttctcggc
      901 ctcgccgccg aatccaaagc gctcttccac gccctcaacg cgctttatcg cgaatatccc
      961 gatcgcatcc cctcccgcac ccacgccttc tggtccagcg ctgcaggctc ctgatcacaa
     1021 cgggcgggtt ccccccacca aatgccgtac actggccagt ctctcctgat ccctcttccc
     1081 aatggagatc ccttcatgct cgtgcgccgt tccctcctca tctttgccct cctctctgcg
     1141 ccactgatgc ttcctgctca ggctcccaat ccgatccacg gcgaaaacgg catcaccctg
     1201 cccgcgcccc ccgtcgccaa agtcgacacc gtcaccgacg actacaacgg caccaaaatc
     1261 gaagatccct atcgctggct cgaagatgcg cacgcgcccg ccacgcgcgc ctggatcacc
     1321 gaagagaaca actacacgca gaaatatctc tcccaactca ccacactgcc caaaatctcc
     1381 agcgaactca ccgcgctgat gcgcgtcgac cagtactcca tccccacgct ccgcgccggc
     1441 aaatatttct tcaagaaacg tctcgccgat cagaatcagg gctccattta cacccgcacc
     1501 ggctggcacg gcaaagatga gctgctcatc gatgccagca aactcagtgc cgaccagaac
     1561 acctccgtca acatggtcga tgtctccgac gacgccaacc tcgtggtcta cgaaattcgc
     1621 gttggcggcg ccgacgaagg ctccgtccac atccgcagcg ttaaaaacgg cagcaacctt
     1681 gcggatgtgc tgcccaccgc gcgctactcc ggcatcagcc ttgcgcccga taactccggc
     1741 ctctactacg ccaaattcac gcatgaaggc accaacgtct ggttccacaa gttcggcacg
     1801 cccgtccata cggatcagaa gctcttcggt ggcgaataca agggccgcca gctcggggag
     1861 ctcgatctga tcagctgcga catcaccgac gaccatcact acctcatcgt ctccattgcg
     1921 cacggcgtgc ctgccaccag ccaggactac ctcgtcaaag acctgcgcaa gaccagcgcc
     1981 gacttcgtcc cgctcgtcta caacgttgag gctcacgtcc gtgtcctcga ggccgccgat
     2041 cgcttcttcc tcatgaccga ctacgaggcg cccaacaccc gcatcgctga ggccactccg
     2101 ggcagcgaag cctccgcatg gaaaaccatc gtccccgaag gcaaagaagt catcgacagc
     2161 tattccatcg tcggcggcaa gctcttcgtc tcgcgtctcc acgacgtcaa aaccgagacc
     2221 accatctaca ccctcgacgg caaacaaatc ggaaccctca gctatcccgg catcggcacc
     2281 ggcaccgttg tctacggcta ccccacccaa accgacggct tctacacctt cgactccttc
     2341 atcctcccgc ccaccatcta ccgctacgac acaaagaccg gccgctccga cgtcttcgcc
     2401 gcgcccaaag tcccgttcga ctcctcgcag tacgaagtca ctcaggtttt cttcaattcc
     2461 aaagacggca cccgcgttcc gatgttcatt gccggcaaaa aaggcctgcc tcgcgacggc
     2521 tccgcacggc tgctgatgac cggctacggc ggtttcgacc tcgccgagct ctccacatgg
     2581 aaccccgagt acgcctggtg gatggaacag ggcggatggt tcgccctccc gaatctccgc
     2641 ggcggcaatg aatacggcga gtcctggcac aaggccgc
//
LOCUS       DASZYN010000449         1668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1114889, whole genome shotgun sequence.
ACCESSION   DASZYN010000449 DASZYN010000000
VERSION     DASZYN010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114889"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VHE33_11595"
     CDS             complement(<1..452)
                     /locus_tag="VHE33_11595"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVT98140.1"
                     /translation="MDRVKRLLISGASGLIGSALRSSASAKGIEVQTLVRRHREVNTG
                     AIYWNPGETNRGVHPAALEGFDAVVHLSGATIARRWSPQYRETIVSSRVRSTQVLCDV
                     LGKVRRKPSVLLCASAVGIYGDRADEVLTEDSTPGSGFLAETCKSWESA"
     gene            674..1396
                     /locus_tag="VHE33_11600"
     CDS             674..1396
                     /locus_tag="VHE33_11600"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HVT98141.1"
                     /translation="MESKTKPTALNLENAPPAALSAESFIAEKRIGERIRRLRLKRSM
                     GLVELGRHTGLSASFLSQLETGRVVPTLRNLARVAMVFGRDLSYFFEPEPHPLFRVHR
                     RSERVRLPQSGVDDPTYYFESLAYMVPDRQLDPYFAEFVPVRKGNEIRPHVHPGYEFL
                     YLLQGELEIRHADRAHALEAGDSVYFDAATPHSYRCGGPAPAVALIVTMHQMTPQAAA
                     GVRPIPPQRVTAAAPQPAETEQ"
     gene            complement(1398..>1668)
                     /locus_tag="VHE33_11605"
     CDS             complement(1398..>1668)
                     /locus_tag="VHE33_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT98142.1"
                     /translation="ARVQSATEAIISVEDKGRGIPPEKLDIIFERFQQVDASDSRDMG
                     GTGLGLAICRNIVRQHGGRIWAESVVGKGSTFYFTVPRSAARQDA"
BASE COUNT          327 a          504 c          488 g          349 t
ORIGIN      
        1 gccgactccc agcttttgca cgtctcggcg aggaagcccg agcctggcgt gctgtcttcc
       61 gtgaggactt catcggcgcg gtcgccatag atgccgaccg ccgaagcgca gagcaacacg
      121 gatggctttc gccggacctt cccgaggacg tcacacaaca cctgcgtgct gcgcactcgg
      181 ctggagacaa tcgtttcgcg atactgcgga ctccagcgcc gcgcgatcgt ggctccgctg
      241 aggtggacca cggcgtcgaa gccttccaat gcggccggat ggacgcctcg attcgtttcc
      301 cccggattcc agtagatggc gccggtgttt acctcgcgat ggcggcggac taatgtctgg
      361 acctcgatgc ccttcgcgga tgcactgctt cggagagctg agccgatcag tccagaggca
      421 ccgctgatga ggagtcgctt tacgcggtcc atttcttatc tttcacatga agaagcatgc
      481 acaaatatat gtccctggtt catataaata cgaaagaaga atgctcttct cgctgggttc
      541 aagaggttta ggtgaattcc acatgatttc cacagttttg cgactattca caacatcaaa
      601 aatacttgtg attcaggaaa cataatggta gcgtcattca tcgcagatcg ctggatgctt
      661 cgcgggtaaa tccatggaaa gcaagaccaa acccaccgct ctcaatctgg aaaacgcgcc
      721 ccccgccgct ctttccgccg aatcgtttat cgcagaaaaa cgcattggcg agcgaatccg
      781 ccgcctccgc cttaaacgct cgatgggcct ggtcgagctg gggcgtcata ccgggctttc
      841 agcgagcttc ctttcgcagt tagaaacggg gcgcgtggtg cccacgctgc gcaatcttgc
      901 gcgtgtcgcc atggtgttcg gacgggatct ttcctacttc ttcgagccgg agccgcatcc
      961 gctctttcgg gttcatcgca ggtcagagcg tgtgcggttg ccgcaatcgg gcgtcgacga
     1021 tccgacgtac tacttcgaaa gcctcgctta catggtgccc gaccggcaac tcgatcctta
     1081 tttcgccgaa ttcgtaccgg tgcgcaaagg caacgagatc cgaccccatg ttcacccggg
     1141 ctacgagttt ctttatcttc ttcagggaga gcttgagatc cgccatgcgg accgggcgca
     1201 cgctctggaa gccggggaca gtgtctattt cgacgcagct acgccgcatt cctaccgatg
     1261 cggaggcccg gcccctgctg tagccctgat tgtgacgatg catcagatga cgccgcaggc
     1321 ggcggcgggg gtgcgaccga ttccgccgca gagggttaca gcagccgcgc cccagccagc
     1381 ggagacggag cagtaggcta agcgtcctgc ctggcagcgg atcggggaac ggtgaagtag
     1441 aacgtgctgc ccttgccgac gacgctttcg gcccagatac gtccgccatg ctgacgcacg
     1501 atgttgcgac agatggcgag accgagtccg gtgccgccca tgtcgcgcga atccgacgcg
     1561 tcgacctgct ggaatcgctc aaagatgatg tccagttttt cagggggaat accgcgcccc
     1621 ttatcttcga ccgagatgat ggcctcggtg gcgctctgga cgcgagcg
//
LOCUS       DASZYN010000450         7990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1044860, whole genome shotgun sequence.
ACCESSION   DASZYN010000450 DASZYN010000000
VERSION     DASZYN010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044860"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VHE33_11610"
     CDS             complement(<1..239)
                     /locus_tag="VHE33_11610"
                     /inference="COORDINATES: protein motif:HMM:NF012510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HVT98143.1"
                     /translation="MRKVLIANRGEIALRVICACKEMGIRTVAIYSEADRHSLHARFA
                     DEAICIGPPRSAESYLNVPAVISAAEIADVDAIHPG"
     gene            complement(242..760)
                     /gene="accB"
                     /locus_tag="VHE33_11615"
     CDS             complement(242..760)
                     /gene="accB"
                     /locus_tag="VHE33_11615"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HVT98144.1"
                     /translation="MKAEELRELKELIEFLKENKIGEFDLERGDLKVRIKFAQEGGAD
                     VAGLARALASQAHAAPAAAAPVVLPSAQAAVSGPSAAAPAAAEADDEASLHIVKSPIV
                     GTFYESPSPGSPAFIKIGDQVEIGQVLCIVEAMKLMNEIESDGAGEIVKRFVQNGQPV
                     EYGQRLFAVRPR"
     gene            complement(788..1885)
                     /locus_tag="VHE33_11620"
     CDS             complement(788..1885)
                     /locus_tag="VHE33_11620"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HVT98145.1"
                     /translation="MHNKADISKRWRALRRAMAERGLASLLVKNLPDVRWLCGFTGSN
                     AALAVTSKSAVLFTDGRYTTQAREETRGARVRIAEKSALKECCALLSESAGKAWFDPQ
                     ATTVADLALMRSGVEKKKSRGFFVALEKPLISALRMVKDAGELRLMEQAAGLGCTLFE
                     TMLPRIEAGMTEAALAAELEFEARKRGAEGMSFETIVASGARSALPHGRATEAKLPRK
                     GFVTMDFGVILKGYCSDMTRTVHLGSATREERAAYDAVLEAQQAAVDAVKPGATCGEV
                     DEAARNVLEESGWGKYFTHSTGHGVGLEIHEQPRIAAGQETRLEAGMVITIEPGVYLP
                     GKFGIRIEDMVAVTAKGCRILTPVTKSWIEL"
     gene            2044..2550
                     /locus_tag="VHE33_11625"
     CDS             2044..2550
                     /locus_tag="VHE33_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98146.1"
                     /translation="MNFLRRLISSFVPAAILLVPLAASAQTILHAPEASKLLPDSVYY
                     AGKSANAQLRNSAGIRYSDNHYVFAVLVDTSGYSTSIQEKYQGYLLTETSIEIDGKSL
                     PAGAYGMGFVSERFVVTDIGGHEILQARASHDEDMQHPMPLQILQGPSPDHYRLCLGR
                     SCVILRRP"
     gene            2665..3615
                     /locus_tag="VHE33_11630"
     CDS             2665..3615
                     /locus_tag="VHE33_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98147.1"
                     /translation="MSRTVRLFALAAAGMFCLVPFALSQNEGQGEGTATVTILPKDSH
                     TSSPDISQQTIHVEVNGKQSQITSWQPLRGAQARVEVVLLIDSGARTSLGRELEDIRQ
                     FIQSLPPNAKVALASMENGRAVLAGPLSSDRAKVIEQIHLPAGIPGQSASPYFCLSDL
                     AKRWPSNDPAARREVIMITDGVDYYHPQYDPDDPYVQTAIRDSIRAHLVVSSIYWRSA
                     GRFDRTWYANNSGQSLLQQVTSATGGNSYWIGMGNPVSLSPYFDDFNRRLRNQYELGF
                     TAPTSGRPEMANLKIKINAHNVKVTSPEKTWLAPGQMAQR"
     gene            complement(3690..4469)
                     /locus_tag="VHE33_11635"
     CDS             complement(3690..4469)
                     /locus_tag="VHE33_11635"
                     /inference="COORDINATES: protein motif:HMM:NF011405.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HVT98148.1"
                     /translation="MYRRLDAERMPQHVAIIMDGNGRWAGKRSLKRFFGHQQGAEAVQ
                     YVVETASRIGLPWLTLYAFSLENNLKRPRTEVSFLMRLLKSYLTNNVQRMNDNNVRIQ
                     YVGRTEELPEEVRERMRYAEEATRRNTGTTLTLALNYGARSELVDAFRRLVADVQRNG
                     LRAEQISELDIHRHLYTAHMPDPDLLIRTSGEMRVSNFLLWQIAYTEIYVTDRLWPDF
                     RGIHLLDAIEDYQRRERRYGGLSDGTESDDVESLPANQMVG"
     gene            complement(4801..5397)
                     /locus_tag="VHE33_11640"
     CDS             complement(4801..5397)
                     /locus_tag="VHE33_11640"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVT98149.1"
                     /translation="MWLLFACRTIPDRTLWRSVQPMATTLATVEAREVLRCESCGLVQ
                     FRTSNSICRRCRKPLEIEEPEPLSPQLVIDNIAGAEAEGIDVARAVREVRHDRCLSQR
                     QLAGRMAVPRTYISKIENGKAVPTLSSLQRLAHALEVGICDLLRDGQSRRAREAAAVL
                     ADPFLAEIAPLVAQLDAFQRAMFLNQVREMAGGRRRTA"
     gene            5514..6134
                     /locus_tag="VHE33_11645"
     CDS             5514..6134
                     /locus_tag="VHE33_11645"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HVT98150.1"
                     /translation="MPDSSFTRERAWSLLNEHTQSPSLIKHALAVESCVRSYAERHAD
                     STGLTGDDRGALLETWSATALLHDFDYEKHPSQEEHPFVGIAILTEQGWPETVRTAIL
                     GHAEYSGVPRESQLAKTLFACDELAGFLTACSLVKPTKSIHDVDVAGVKKKMKDKAFA
                     RGVNRDDIVKGASELDIPLDDHIANCIAAMRVNAETLGLQGTASQG"
     gene            6213..>7990
                     /locus_tag="VHE33_11650"
     CDS             6213..>7990
                     /locus_tag="VHE33_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HVT98151.1"
                     /translation="MPTLRTLNDIFLAIADSPRDRAMLFQDAAGAWQPISALQVYQRV
                     RALAETLRSWGVRKGDRVAILAENRWEWAVADFACLAIGAIDVPIYPNLLTDHIVPLL
                     VDSGSRILFVSTRAQYEKIAPRSAATPLEHIVIMDDDPTATGAESFSSLIASGSPQPT
                     PRDPAFDEATRAIQPSDLATLIYTSGTTGEPKGVLLTHGNIASNINYSLAEFDLGPAD
                     SCISFLPLSHITARHLDYALYVKDVLVAYCSSFDKLPQALLAVQPTILVAVPRVFEKV
                     RQVAEQKASESPIKRRLFAWALATGAKHRDEIAAGRTPSSLSWKLANALVYKKLKAGF
                     GGRVRYAIAGGAPLGLDTAHWFASAGIRILEGYGLTETSPVIALNTENANRMGSVGKP
                     LPNVECRIAADGELLVRGPSIFQGYWKNRHGEEETIDAEGWFHTGDIARIDKDGFLYI
                     TDRKKELLKTSGGKLIAPQPIENKLKTSVLVGNCALVGDRRKFAAAIILPNFAALEPW
                     ARERGIPFDAREQLVVYDEVRAEFQAIVDRVNATLANFETIKRFRLVHEEWAVDSGEL
                     TPSMKLRRRVITQKYAREIAEFYADES"
BASE COUNT         1546 a         2831 c         2046 g         1567 t
ORIGIN      
        1 ccggggtgaa tcgcatctac gtcggcgatc tccgcagcgc tgataaccgc agggacgttc
       61 aggtagctct ccgccgaacg cggcggtccg atgcagatgg cctcgtcagc aaagcgcgcg
      121 tgcagcgaat gccggtcggc ctcgctgtag atggctaccg tccgaatgcc catctcttta
      181 caggcacaga tcacgcgcag cgcaatttcg ccgcgattgg cgatgaggac tttacgcatg
      241 gttatcgggg acgcaccgca aacagccgtt gaccgtattc caccggctgt ccgttctgca
      301 cgaaccgttt cacgatttcg ccggcgccgt cactctcaat ctcattcatc agcttcatcg
      361 cttccacgat gcacagcacc tgacctatct ccacctgatc gccgatcttg ataaaagccg
      421 gagagcctgg cgacggcgac tcgtagaacg tgcccacgat gggtgacttc acaatgtgca
      481 gcgaagcttc gtcatcggcc tccgccgccg caggagccgc ggcgctcggc ccagagaccg
      541 ccgcctgtgc cgatggcagc accaccggcg ccgctgcagc cggagccgcg tgggcctgcg
      601 acgccaacgc ccgcgccagc cccgccacat ccgctccgcc ctcctgcgca aacttgatgc
      661 gaaccttcag atcgccgcgc tcaaggtcga actccccaat cttgttttcc ttgaggaact
      721 cgatcagttc cttcagctct ctcaattctt cagctttcat cgtctcgtct cgcggcctct
      781 cccgtcctca caattcaatc cagctcttgg tcacaggcgt cagaatccgg cagcccttcg
      841 ccgtcactgc aaccatatct tcgatgcgga ttccgaattt ccccggcaga taaacgcccg
      901 gctcaatcgt aatcaccatc cccgcttcca gccgcgtctc ctgtcccgcc gcaatccgcg
      961 gctgctcgtg aatctccaaa cccacgccgt gcccggtcga atgcgtgaag tatttgcccc
     1021 aacctgactc ctccagcaca ttccgcgcgg cctcatccac ctcgccgcac gtcgccccag
     1081 gcttcaccgc gtccactgct gcttgctgcg cttccagcac cgcatcatac gcggcgcgtt
     1141 cctcacgcgt agcgctgccc aggtgcaccg tgcgcgtcat atcggagcaa taacccttga
     1201 ggataacacc gaagtccatt gttacgaatc ctttgcgcgg cagcttcgct tcggttgcgc
     1261 gcccgtgcgg cagcgccgac cgtgcccccg atgcaacgat cgtctcgaac gacatgccct
     1321 ccgccccgcg cttccttgcc tcgaattcca gctccgccgc cagcgctgcc tccgtcatcc
     1381 ccgcctcgat gcgcggcagc attgtctcga acaacgtgca ccccagcccc gccgcctgct
     1441 ccatcagccg cagttcgccg gcgtccttca ccatccgcag cgcggagatc agcggcttct
     1501 ccagcgcaac aaagaatccc cggctcttct tcttctccac tccggaacgc atcagcgcca
     1561 gatccgccac tgtggtcgcc tgcggatcga accaggcctt ccccgccgac tccgacagca
     1621 gcgcgcagca ttccttcagc gccgacttct cagcgattcg cacccgcgcc ccacgcgtct
     1681 cctcccgcgc ctgcgtcgta tagcgcccgt cggtgaacag caccgcgctt ttcgacgtca
     1741 ccgccagcgc tgcgttcgat cccgtgaatc cgcacagcca ccgcacgtcc ggcaggttct
     1801 tcaccagcag cgatgcaagg ccgcgctctg ccatcgcgcg ccgcaacgcc cgccaacgct
     1861 tcgaaatgtc ggccttgttg tgcatcaact tcgatactaa ttactttctc tgcagtttcc
     1921 gaaattccgc cgcgattggg cgaatcgccg caattacccc accaggggcc tgaaccatga
     1981 caggcgctgt acgctgaacg ccattcgctg tacgctgttc tcgccagttc aggaggccat
     2041 gcaatgaact ttctgcgccg tcttatctct tcctttgttc ccgccgcgat tttactggtt
     2101 ccgctcgcag cctctgccca gaccatcctt catgcccccg aagcctcgaa gctcctgcct
     2161 gattccgtct attacgcggg gaaatccgcg aacgcacagc tgcgcaattc cgcgggcatt
     2221 cgttactctg ataaccacta tgtcttcgcc gttctcgtcg acacctccgg atactctacg
     2281 tccattcagg agaaatacca gggctatttg ctgaccgaaa cgtccatcga aatcgacggc
     2341 aagagtttgc ccgccggtgc ttatggcatg ggttttgtta gcgaacgctt tgttgtgaca
     2401 gacataggcg gccacgaaat tcttcaggcg cgcgcctctc acgatgagga tatgcagcat
     2461 cctatgccac tgcagatcct gcagggcccc tccccggatc attaccgcct ctgcctcggc
     2521 cgtagttgcg tcattctgcg gcgtccttag gaagaacgcc ctcgccggcg acgatttgca
     2581 ctcattgtcc atctaagtaa tgagcagatg gcgttctgtt ttttcgcacg gacaagcgag
     2641 ccaccatcgg cagaagaggg acacatgagt agaacggttc gattgtttgc gcttgctgcc
     2701 gcgggaatgt tctgccttgt cccgtttgcg ttgtcgcaga atgaaggtca gggcgagggc
     2761 accgccaccg tgaccatcct gcccaaagat tcccacacca gttccccgga tatctcccaa
     2821 cagacgattc acgtcgaggt caatggcaag cagagccaga tcaccagttg gcagcccctg
     2881 cgcggagccc aggctcgtgt cgaagtcgtc ctgcttatcg acagcggcgc gcgtaccagc
     2941 cttggtcgag agctggagga catccgccag ttcatccaga gcctgccgcc caatgcaaag
     3001 gtagctcttg cttccatgga gaacggccga gccgtgctcg cgggtcctct ttccagcgat
     3061 cgcgcgaaag tgatcgagca aatccatctt ccggccggca ttccgggcca gagtgccagc
     3121 ccatattttt gcttgtccga tctcgcaaaa cgctggccct ccaatgaccc tgccgcgcgt
     3181 cgcgaagtca tcatgatcac cgatggcgtt gattattacc acccgcaata cgatcccgac
     3241 gacccctacg tccagaccgc catccgggat tcgatccgcg cccacctggt tgtctcgtcc
     3301 atttactggc gcagcgctgg ccgcttcgat cgtacctggt atgcaaataa ttcgggacaa
     3361 agccttctcc agcaagtcac cagcgcgacg ggcggcaatt cgtactggat cggcatgggc
     3421 aatcctgtct cgctttcgcc ctatttcgac gatttcaatc ggcgcctcag gaatcagtac
     3481 gagctcggct tcactgcacc cacgtccggg aggccggaga tggccaacct gaagatcaaa
     3541 atcaatgccc acaatgtgaa agtcaccagc ccggaaaaaa cctggctcgc cccaggacaa
     3601 atggcacaaa gataacgggc tcttcccaga caaaaaggcg catcgttgcg atgcgccttt
     3661 ttccgttccc tgtttcgtgc gctctgctgt tatcccacca tctgattggc tggcaggctt
     3721 tccacatcgt ccgattcggt gccgtcgctc aggccaccat agcgccgctc gcgccgctgg
     3781 tagtcttcga tagcgtcgag caggtggatc ccgcgaaaat ccggccacag ccggtcggtc
     3841 acatagatct ccgtgtacgc aatctgccac aggagaaaat tcgacaccct catctcgccc
     3901 gacgtccgaa tcagcagatc cgggtccggc atgtgcgccg tgtacagatg ccggtgaata
     3961 tccagctcac tgatctgctc tgcccgcagc ccgttgcgct gtacatcggc gaccagacgg
     4021 cggaaagcat ccaccagttc cgatcgcgcg ccatagttca atgccagggt cagtgtcgtg
     4081 ccagtattgc gccgcgtggc ctcctcggcg tagcgcatcc gctcccgaac ctcttccgga
     4141 agctcttcgg tccggcccac atactggatg cgcacattgt tatcgttcat gcgctgcacg
     4201 ttgttggtca gatagctctt cagcagccgc atcagaaagc tcacttccgt gcgtggccgt
     4261 ttcaaattgt tctcaagcga aaacgcatac aaggtcagcc atggcagccc gatccgtgaa
     4321 gcggtctcta ccacgtactg caccgcctcg gcaccctgct gatggccaaa gaaccgcttg
     4381 aggctgcgct tgccggccca gcgtccgttg ccgtccatga tgatggccac gtgctgcggc
     4441 attcgctccg catccaggcg gcggtacacc gctgcctcat ctgcaggcag ttcatgaact
     4501 cggctggagt tgagtaacaa cgcgtccttt cgagtggctc ccgcggggga cggacctggc
     4561 acaggtcctt accccaacgt gccggcgcgc gtcgcagcac gccagaggca gcggaagctg
     4621 ggagcggctc cctctctgcc agattataca gagccaaaca aaacggggac gcaggttgcc
     4681 tgagccccgg gaacgaaaag gaacgctctg cgaatggcca catccctggc gttggggtgt
     4741 ggccataccg gtgctgcagg cactgattgc tctcgtctgg ctttgagcgg aagggctctg
     4801 ttacgccgtc cgtcgccgtc cacccgccat ttcgcggacc tgattcagga acatggcacg
     4861 ctggaaggca tccagttgcg ccactaacgg agcaatttcc gcaaggaacg gatccgccag
     4921 aaccgcagca gcctctcgtg cccggcggct ctgtccatcg cgcaacagat cgcagatgcc
     4981 cacctccagc gcatgtgcca gccgctgcaa agacgacaac gtaggcaccg ccttgccgtt
     5041 ctcaatcttg gaaatgtaag tacgcggcac ggccatgcgc ccggcgagct gtcgctggct
     5101 caggcaacga tcgtgtcgta cttcgcgcac cgcgcgggcc acgtcgatac cttctgcttc
     5161 tgcccccgct atgttgtcga tgaccagttg tggggacagc ggctccggct cttcgatctc
     5221 cagcggcttg cggcagcgcc tgcagatcga gttcgacgta cgaaactgaa ccaggccaca
     5281 ggattcgcaa cgaagtacct cgcgggcctc aacagttgct aacgtagttg ccataggttg
     5341 tacggaacgc cagagcgtgc gatccgggat ggttcgacag gcaaacaaca gccacacgaa
     5401 accaaccttt gagatacatt gcctggaggg tatccttctt gtcaagtaaa aactgtgagt
     5461 ctctccaaaa attcccaggg aatgccaggg aaacccaaaa ggaattcgac ccgatgcctg
     5521 actcttcttt cacgcgcgaa cgcgcctggt cgctgctgaa tgagcacact caatccccga
     5581 gcctcatcaa acatgcgctc gctgtcgaaa gttgcgtgcg ttcctatgcc gagcgccatg
     5641 ccgactccac tggccttacc ggcgacgatc gcggcgcgct tctggaaacc tggtccgcta
     5701 ccgcgctcct tcacgacttc gactacgaga aacatccctc gcaggaagag cacccctttg
     5761 ttggcatcgc catcctcacc gagcagggct ggcccgaaac ggttcgcacc gccatcctcg
     5821 gccacgccga atattccggg gtcccgcgtg agtcccagct cgccaaaact ctcttcgcct
     5881 gcgatgaact cgccggcttt ctgaccgcat gctccctcgt gaagccgacg aaatccattc
     5941 acgatgttga cgtcgctggt gtgaagaaaa aaatgaaaga taaggccttc gcccgcggtg
     6001 tcaatcgcga cgacattgtc aaaggagcat cggaactcga cattcccctc gacgaccaca
     6061 tcgccaactg catcgcggcg atgcgcgtca acgccgagac cctcggactc cagggcacag
     6121 cgtcacaagg gtgaatccat cgtgtgccgc agtcgcacca tgttttcgac gccctgtacc
     6181 ctccaccctg taccctgtta aattgatccc gcgtgccaac gctgcggacc ctcaacgaca
     6241 tcttcctcgc gattgcggac agcccccgcg atcgcgcgat gttgttccag gatgctgcgg
     6301 gagcatggca gcccatctct gccctccagg tctatcagcg cgtccgcgcc cttgctgaga
     6361 cgcttcgctc ctggggggtt cgcaaagggg accgggtcgc gatcctcgcc gaaaaccgct
     6421 gggagtgggc cgtcgccgac ttcgcctgcc tcgcgatcgg cgccatcgat gttcccatct
     6481 accccaacct tctcaccgac cacatcgttc ctctcctcgt cgactccggg tcgcgcatcc
     6541 tcttcgtctc cacccgtgcc caatacgaaa agatcgctcc tcgctccgcc gccacaccgc
     6601 tcgagcacat cgtcatcatg gacgatgacc ccacagcaac aggcgcagag tccttctcct
     6661 ccctgattgc ttccggctca ccgcagccaa ccccgcgcga tcccgcattc gacgaagcca
     6721 cccgcgccat ccagccctcg gacctcgcca ccctcatcta cacctccggc actaccggcg
     6781 agcccaaggg cgtccttctt acccacggca acatcgcctc caacatcaac tactcgcttg
     6841 cagagttcga cctcggcccc gccgactcct gcatctcctt cctcccgctc tcgcacatca
     6901 ccgcacgcca cctcgactac gcgctctacg ttaaggacgt gctcgtcgct tactgcagca
     6961 gcttcgacaa actcccccag gccctgctcg ccgtccagcc caccatcctt gttgctgtgc
     7021 cccgtgtgtt tgaaaaagtc cgccaggtcg ccgagcagaa agcctccgaa tccccgatca
     7081 agcgccgcct cttcgcctgg gctctcgcca caggcgccaa acatcgcgac gaaatcgctg
     7141 ccggccgcac cccctcgtct ctctcctgga aactcgccaa cgccctggtc tacaaaaagc
     7201 tcaaggcagg attcgggggc cgcgttcgct acgccatcgc cggcggcgcg ccccttggcc
     7261 tggacaccgc gcactggttt gcgagcgcag gcattcgcat cctcgaaggc tacggcctca
     7321 ccgaaacctc gcccgtcatc gccctcaaca ccgaaaacgc gaatcgcatg ggctcggtcg
     7381 gcaagccgct ccccaacgtc gagtgccgca tcgctgccga cggcgaactc ctcgtgcgcg
     7441 gccccagcat ttttcagggc tactggaaga atcgccacgg cgaagaagag accatcgatg
     7501 ccgaaggctg gtttcatacc ggtgacatcg ccagaatcga taaggacggc ttcctttaca
     7561 tcaccgaccg caagaaggaa ctcctcaaaa cctccggcgg caagctcatc gccccgcaac
     7621 ccatcgagaa caagctgaaa accagcgtgc tggtcggcaa ctgcgccctg gtgggcgacc
     7681 gccgcaagtt tgccgcggcc atcatcctgc ccaactttgc cgcactcgaa ccctgggccc
     7741 gcgaacgcgg cattcccttc gacgcccgcg agcaacttgt cgtttacgac gaggtccgcg
     7801 ccgaattcca ggccatcgtc gaccgcgtca acgccaccct cgccaatttc gagaccatca
     7861 agcgcttccg cctcgtgcac gaggagtggg ccgtcgactc cggcgaactc acccccagca
     7921 tgaaactccg ccgccgcgtc atcacccaga aatacgcccg cgaaatcgcc gaattctacg
     7981 ccgacgaatc
//
LOCUS       DASZYN010000451         5032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_984648, whole genome shotgun sequence.
ACCESSION   DASZYN010000451 DASZYN010000000
VERSION     DASZYN010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984648"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VHE33_11655"
     CDS             complement(<1..293)
                     /locus_tag="VHE33_11655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT98152.1"
                     /translation="MSAIAFLDRTNISIAGVEISREFRLDNSQLGWIFSAFLVGYALF
                     QIPGGLISEKFGARRVLAFSVLWWGLFTGLTALVRPGIGRSIAFLIGIRFLLGV"
     gene            complement(624..3290)
                     /locus_tag="VHE33_11660"
     CDS             complement(624..3290)
                     /locus_tag="VHE33_11660"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HVT98153.1"
                     /translation="MKKFALRAISVGALLFAAVATSAIAQQTATQPWLNPKLSPEQRA
                     QEIVSRMTLEEKASQLVNDARPIPRLGVPAYNWWSEALHGVIDSGVTEFPEPIGLAAT
                     FDVPGVHAMATDIGIEGRIKHEQDVKAEHKGIMGGLDFWAPNLNIFRDPRWGRGQETY
                     GEDPFLTGRMGVAFVTGMQGDDPTYYRVIATPKHYVVHSGPEPTRHFADVDVSRHDEL
                     DTYEPAFRAAVVDGKAASVMCAYNGINGQPACANQFLLQDQLRGKWGFQGYVVSDCDA
                     VRDIFTGHHYRPTQPQSSAISLERGMDNECYGANTEYPKDNSDYMPFVEAVQQGYLSQ
                     NAMDTTLVRLFTARIRLGMFDPPDAFPYGKIDEKELDSAEHRALAHKLADESMVLLKN
                     DGTLPLKPGIRNIAVVGPLADQTRVLLGNYAGRPTHIVSMLDGLKAEFPNAKITFAAG
                     TQFLRSEGDPLPNGLLTTPDGKPGLKAEYTKWQGRQMEPGNRPAPLATRVEPNVNLSG
                     NNLPAELSGTKSVGVHWTGFLTAPESGDYLVGVRADGFAVLTVDGKPIARARTQGVEN
                     KVGRVHLEKGHKVALELMYGNPGTDEPHAQLIWAKVNNAPSPEALAAARNADVVIAVV
                     GITSELEGEEMPVSEPGFLGGDRTSIDLPEPEEELVEAVAATGKPLVVVLMNGSALAV
                     NWEQQHANAVLEAWYSGEEGGTAIADTLSGKNNPAGRLPVTFYTGVDQLPHFEDYSMN
                     NRTYRYFTGKPLYPFGYGLSYTTFTYSDLSVPQAPIHAGDPVNADVTVTNTGKIAGDE
                     VVQLYLKFPSVAGAPNIALRGFERVHLDPGASQKVHFELKNRDLGMVTEDGHPIIPGG
                     DYTISIGGGQPDTGAPAVAGHFHVEGQVGLPE"
     gene            complement(3392..4504)
                     /locus_tag="VHE33_11665"
     CDS             complement(3392..4504)
                     /locus_tag="VHE33_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVT98154.1"
                     /translation="MQPKLLFASMALFSLILAGAVPSFAQVQTEVPAVIPNAKPVTVE
                     HIKVHSPAIEGNLEGESADRDVIVFLPPSYNKDKHRRYPVVYALHGYSIGAEQWTHEI
                     HVPQTIEGAFAQGSKEMIVVLPDSKTAYNGSMYSSSVTTGDFEKFIYRDLVAYIDAHY
                     RTIPDRMSRGLVGHSMGGYGATRIGMKHSDVFGSLYIMSPCCMSPRPAGPPPGADAAK
                     FADAEKQFETAVAGMKSAADSASMQFFQRAQLASAAAWSPNPKNPPLYLDLPMKDGVS
                     QPDVLAKWTANAPLAFIDQYIDNLKEYRAIAEDVGDQDGLRFDTQKLHAILDNYGIQN
                     SFEMYHGTHTSAVADRFQNHVMPFFSQNLCTTEHCR"
     gene            complement(4660..>5032)
                     /locus_tag="VHE33_11670"
     CDS             complement(4660..>5032)
                     /locus_tag="VHE33_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98155.1"
                     /translation="VTFDHLDQFSYIGGFSGAANPFLMGADKLDTKTAFHGAMADPSA
                     FAKRVHLLWIGVGTDEPDRMKSGLEQLHSSLDEGKIQHIFYESPGTSHEWQTWRRDLK
                     DFAPRLFQPVDRQTVAAQRKD"
BASE COUNT          964 a         1465 c         1554 g         1049 t
ORIGIN      
        1 acgccgagca gaaatcggat gccgatcagg aaagctatgc ttcggccgat tcctggacgg
       61 acgagggcag ttaatcctgt aaagagtccc caccagagga cgctgaacgc cagaacgcgg
      121 cgagctccga atttctccga aataagcccg ccaggaatct ggaataaggc gtagccaacg
      181 agaaaggcac tgaagatcca gccgagctgg gaattgtcga ggcggaattc tcgactgatc
      241 tccacgccgg caatcgagat attggtgcgg tcgaggaacg cgatcgcact caatacgagc
      301 aaccagcaaa cgagccaata gcgcaccggt ggaagcaggc gagcgcgccc gactggatgg
      361 ctgagcctct gtttgactgg cgcccgatcg cgagagcgat tcaagctaag ttcctccagc
      421 agaaatgctg cagcgagtcc gagcgcctac ctgtcgttca agatcctatc cccacggcct
      481 gtgattttct gttacgccga gccaatgatc ggctgggagg ggcagccgac gatgagccgg
      541 atcacacgct tgccctcgcg gtccaacagg ctttgtacct tccctttggt tttccatcct
      601 tttcgccgtt cacgtcccgg atcctactcc gggagaccaa cctgaccctc aacatggaag
      661 tggccggcga cggcgggtgc gccggtatcc ggctgaccac caccgatgct gatggtgtaa
      721 tcgccaccag ggatgatcgg atggccatct tcggtcacca tgcccaggtc gcggtttttc
      781 aactcgaagt gaaccttctg gctggctccg ggatcgaggt gaacgcgttc gaatccgcgc
      841 aacgcgatat tcggcgctcc cgccacggaa gggaatttga gatacagctg caccacttca
      901 tcgccggcga tcttacccgt gttcgttacg gtcacatcgg cattcaccgg atctcctgca
      961 tggatgggag cctgcggcac gctcagatcg ctataggtaa atgtggtgta actcaggccg
     1021 tatccaaaag gatagagcgg cttacccgtg aagtaccggt aagtgcggtt gttcatggag
     1081 tagtcttcga agtgcggcag ctgatcgacg cctgtgtaga aggtgaccgg cagccggccg
     1141 gcggggttgt tcttcccgct tagcgtgtcg gcgatcgccg tcccgccttc ctcgccggaa
     1201 taccaagcct cgagcacggc gttggcgtgc tgctgctccc agttcaccgc gagcgcgctg
     1261 ccgttcatca gcacaaccac gagcggcttg ccggttgcgg cgacggcttc gaccagctcc
     1321 tcctccggct caggcagatc gatgctggtg cggtcgcctc cgaggaatcc cggctcactg
     1381 accggcattt cctcgccttc cagctcgctc gtgatgccga cgacggcaat caccacatcg
     1441 gcgttccggg cggccgcaag agcttccggc gacggcgcat tgttcacctt cgcccagatc
     1501 agctgagcgt gcggttcgtc cgtccccgga ttgccataca tcagctccag cgcaaccttg
     1561 tgtcccttct caaggtgcac gcggccaacc ttattttcca ctccctgggt tctggcccgg
     1621 gcgatcggct tgccatcgac ggtgagcact gcaaagccat cggcgcgaac gccaaccagg
     1681 taatcgccag attccggcgc agtgaggaaa cctgtccagt ggacgccaac cgatttggtg
     1741 cccgataact cggccggcag gttattcccg ctgagattca cattgggctc gacacgagtc
     1801 gcaagcggag cgggcctgtt gcctggctcc atttgccttc cctgccattt ggtgtactcg
     1861 gctttcagtc caggttttcc gtccggggtg gtcagcagac cattgggcaa cggatctcct
     1921 tcgctgcgca gaaactgtgt tccggcagca aaggtgatct tcgcgttcgg aaattcggct
     1981 ttgagcccgt caagcatgga gacgatgtgc gtcggtctcc cagcatagtt gccgagcagc
     2041 acgcgcgtct gatcggcgag cggcccgaca acggcaatgt tccgaatgcc tggtttcagg
     2101 ggcagggtac cgtcgttttt gaggagcacc atcgattcgt ccgcgagttt gtgggccagc
     2161 gcgcgatgtt ctgcgctgtc cagttccttc tcgtcgatct tcccgtaggg aaacgcatcg
     2221 ggcggatcga acatgcccag ccgaatccgc gcggtaaaca aacgcacaag cgttgtatcc
     2281 atggcgttct ggctgaggta gccctgctgt acggcttcga cgaacggcat gtagtcagaa
     2341 ttgtctttgg ggtactcggt attcgctccg tagcactcat tgtccattcc gcgttcgagc
     2401 gaaatggccg aggactgcgg ctgcgtgggg cggtaatgat ggcccgtaaa aatgtcgcgc
     2461 accgcgtcgc agtcggaaac gacatacccc tgaaaacccc acttgccgcg aagctgatcc
     2521 tgcagcagga actgattggc gcaggcgggc tgtccgttga tgccgttgta ggcgcacatg
     2581 accgacgcgg ctttgccgtc gacaaccgcg gcgcggaagg cgggctcgta ggtgtcgagt
     2641 tcatcgtgtc tgctcacgtc gacgtcggcg aagtgccgcg tcggctccgg cccgctgtgc
     2701 acgacgtagt gcttgggagt tgcgatcacg cggtagtacg tcggatcgtc accctgcatg
     2761 ccggtcacga aggcgacccc catgcggccg gtgaggaacg gatcttcgcc gtaggtttcc
     2821 tggccgcgtc cccagcgggg gtcgcggaag atgttcagat tcggggccca gaagtcgagg
     2881 ccgcccatga tccccttgtg ctcggccttg acgtcctgct catgtttaat gcgcccctca
     2941 attccgatat cggtggccat ggcatgaacg ccgggcacgt caaaggtcgc ggcgaggccg
     3001 atcggctcag ggaactcagt gacacctgaa tcgatgacgc cgtggagggc ctcggaccac
     3061 cagttgtaag cgggaacgcc aagcctggga atgggccggg catcattgac cagctgcgag
     3121 gctttctcct cgagggtcat gcgagagacg atttcctggg cgcgctgctc cggcgacagc
     3181 ttcgggttca gccacggttg agtggcagtc tgctgcgcga tggcggaggt tgcaacggcg
     3241 gcgaagagca gggctccgac agatatcgct cgcaatgcaa atttcttcat gatggttctc
     3301 ctgagcggct cacaaagcat cgtaaacgtt ttctcgcagc tgcgccgcaa tctcgaagca
     3361 ccggcgctgc ctatttgggt cgggctccct gctacctgca gtgttcggta gtgcagaggt
     3421 tctggctgaa gaagggcatg acgtgatttt gaaagcggtc ggcaacggcg ctggtgtggg
     3481 ttccgtggta catctcaaag ctgttttgga tgccgtagtt gtcgaggata gcgtggagct
     3541 tctgggtgtc gaagcgcaga ccgtcctgat cgcccacgtc ttcggcgatc gcgcgatatt
     3601 ccttcaggtt gtcgatgtac tgatcgatga aagcgagcgg cgcattcgcg gtccatttgg
     3661 caagaacgtc gggctgcgag acgccatcct tcattggaag gtcgaggtac agcggagggt
     3721 ttttgggatt cggcgaccac gcagccgcgg acgccaactg agcccgctgg aagaactgca
     3781 tggaagccga atcggcggcg gacttcatgc cggcgacggc agtctcgaat tgtttctccg
     3841 catccgcgaa cttagccgcg tcggctcccg gcggcggacc ggcggggcgg ggcgacatgc
     3901 aacatgggct catgatgtag aggctgccaa agacgtcgga atgcttcatt ccgatgcgtg
     3961 tggctccgta tcctcccatg gaatggccga cgagaccgcg actcatgcga tccgggatgg
     4021 tgcggtaatg cgcatcgata taggcgacca gatcccggta aatgaatttc tcgaaatcgc
     4081 ctgtcgtcac agagctggag tacatcgagc cgttataagc ggtcttcgag tcaggcagga
     4141 caacgatcat ctctttggag ccctgcgcga aggcgccttc gatggtttgc gggacgtgaa
     4201 tctcgtgggt ccactgctct gctccaatgg agtagccgtg gagggcgtac acgacgggat
     4261 agcggcgatg tttgtctttg ttgtagctgg gcggcaaaaa gacaatgacg tcccggtcgg
     4321 cggattcccc ctcgagattg ccttcgatcg cgggcgagtg caccttgatg tgctcgaccg
     4381 tgacgggctt tgcgttgggg atgaccgccg ggacttccgt ctgcacctgg gcgaagctcg
     4441 gtactgcacc tgcgaggatg agagaaaaca aagccataga agcgaaaagg agcttaggtt
     4501 gcatcgtcgt cattcactcc ggttcattca ttgtgctgga attacgttca caaatcgtcc
     4561 gtcgtcgtcg tgcccggtca aaaacgaagc aggatcgggc ggtctcccgc ggaatctggt
     4621 gattgcagag atcccggcac ggcagccggt catgctgcgc tagtcttttc gctgcgcggc
     4681 gaccgtttgt cgatcgacgg gctggaagag tcgcggtgca aagtctttca ggtcgcgacg
     4741 ccacgtctgc cattcgtgcg aggtgccggg cgactcataa aagatgtgct ggatctttcc
     4801 ttcgtcgaga gacgaatgca gttgctccag accggacttc atgcgatcgg gctcatccgt
     4861 tcccaccccg atccaaagca gatgaacccg tttcgcgaag gctgacggat cggccatcgc
     4921 gccgtgaaac gccgtcttcg tatccagctt gtcggccccc atcaggaaag gattggccgc
     4981 tccgctgaat ccaccaatgt acgaaaactg gtcgagatga tcgaacgtca cc
//
LOCUS       DASZYN010000452         6225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_82764, whole genome shotgun sequence.
ACCESSION   DASZYN010000452 DASZYN010000000
VERSION     DASZYN010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82764"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VHE33_11675"
     CDS             complement(<1..908)
                     /locus_tag="VHE33_11675"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HVT98156.1"
                     /translation="MTAKFTAPTSGRALTASIGKNTLFGVVGNLVHVLARIVVVPVMI
                     RHLGLDGYGIWAVLMSIAAYITLGGAGVKSAFQKYVAEATGTGDYDRASRLLSTGTAA
                     VLALSVVVLVPLAIFSHWLAVAVGVPAGFLIPASQAIRILAITAIIANVSQTYQSIMM
                     GAHRIDLREKLNVVLDLLNAAFTIGLLYSGFGLLAIAAVFCAYQLAGGILWYIYARRI
                     LPEVRISSTLVTRSVVKELIRFGASYQLVSMLELLYAAILPVAVLKFFGANAAGVFAL
                     AARLVSFATLIQGSYLQAILSGGSLV"
     gene            complement(968..2554)
                     /locus_tag="VHE33_11680"
     CDS             complement(968..2554)
                     /locus_tag="VHE33_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98157.1"
                     /translation="MNRLIPLFATAVAFVVVGAFIMTRPAFAGTRYIAQTETVFSGGT
                     ACSGQTTITPATFNRITNSPGDVIYLCGTLTGEAGAQLLTPKGDGSAGNPVTIRFDSG
                     AVVEAPYFAASPNGGCGGAICLYGLSYYTVDGQKTGIIENTANGTGLQYNEKTEAIEG
                     FDCNHCTVENLTIENMYVHTSVSDKSADASAQRCISISGSNWLIRNNVMHDAGWCLYN
                     NFQNGEGHVEIAGNTIYNVDHGWMLASQVAGGSSGPFLFHHNTVYGYAAWDTGTADVY
                     HHDGIHCFTSQTHGAGAHITSLAIYDNFFKGPIGQNITAHIFIEGGRGSGATPCADAS
                     SGIAIYNNVAVGDQPDPDGLFGLFSGNFTSQHQGGVYNNTLISTAGTAAGVCFSANSI
                     VSGMTFVNNAVSGCNQLIETDSIPFSADYNQYADGGNNAFVCQGKFYQDAEFSSWQSC
                     IRSDSHSHYRSSLKVNSSYQPETGSPLMHAGTPLNGLCSDTLSLLCSDYAGNPRSAAD
                     RWTVGAWSTPSPAAQGPEGH"
     gene            complement(2682..4298)
                     /locus_tag="VHE33_11685"
     CDS             complement(2682..4298)
                     /locus_tag="VHE33_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98158.1"
                     /translation="MVRNARVLSAAFTAAFLFGICTVAARANDIYIAQAAAGSGNGSS
                     CANAYAYNYFNTSGNWTSAAPTGTKIGPGSVVHICGTITVGANQNALTFQGSGTAGNP
                     VTLYFESGAVVQAPEFPPQGSNGAINTSNQSYVTIDGNGGANGATRGLIQATATGDSG
                     AACPSGSCTYHDDSNAIEADSSSNLVVKGLTISDMYVTTTGVPSGGGGSCIWAHSQVN
                     NWTIDNNVMHDVSWCINLQYDSGTSSGITVSNNEIYNIDHGIAFGGPHSGNTLTNVNI
                     VGNSIHDYSNWDTPGDVWHHDGIHIWGYNNDGSDSISAVNIYNNKFGGCIGKNVTAHI
                     FIEANSGNTKNVSIFNNSLIDTCAGTDNDGLLTTGIDGGYKIYNNTFMGSSGDICMGT
                     SSSPNVTFVNNVVSGCGTLMYIANGGSFASGGLHNNIYAGCSGSNCFAYNGSYSGSFA
                     SWQNATGQDASPSAYVSSANLSPAGIPQAGSPVIGAGSNLTSLSILSLNSDIIGNPRA
                     ATGSWTVGAYSTAGTAPAPPTGLTGTVVQN"
     gene            complement(4600..5466)
                     /locus_tag="VHE33_11690"
     CDS             complement(4600..5466)
                     /locus_tag="VHE33_11690"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVT98159.1"
                     /translation="MSPRFDRAGTLYLAAPVRKVLAGRGDGRIPILMYHSVAEEDESA
                     LQPYFWIATTPARFLQQMTLLHEQGYRTTSLGDAVESLRAGKGTAEKTVVITFDDGYR
                     NFCQHAFPALERYGFTATMFLPTASIGERPQVFNRRECMTWSEVRDLQQRGISFGSHT
                     VNHPQLHGLEPQRIRAEILQSRETIEEKTGVAVDTFAYPYAFPQTDGAFKAMLRSILE
                     ESGYRYGVCTQIGRAGAASDPLFLERLPVNSADDGPLLQAKLAGAYDWVGRLQSVAKR
                     LRKPRVKARTQK"
     gene            complement(5517..>6225)
                     /locus_tag="VHE33_11695"
     CDS             complement(5517..>6225)
                     /locus_tag="VHE33_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HVT98160.1"
                     /translation="VIEDACQAHGAEYFSGKLNRWMKAGTMGHAAAFSFYPGKNLGAL
                     GEGGAVSTNDAAVAAKIKMIREHGQAKKYYHDMEGYNGRLDSIQCGLLQVKLGHLAKW
                     NAARRDRAAEYDRLLAGNDAVVCPYEPSWSRSVYHLYVIRTADRDGLAQHLKEAGIGT
                     GIHYPVPLHQQKAYASMGYKAEDLPVATQLAKEILSLPMFPQLTAEQQRRVAHEIENF
                     TAPRRSAAEPRVLATRS"
BASE COUNT         1205 a         1992 c         1772 g         1256 t
ORIGIN      
        1 tagaccaggc tgcctcccga gagaatggcc tgcagatacg agccctgaat cagcgtcgca
       61 aagctcacca ggcgcgcagc cagcgcaaac acacccgcag cattcgcgcc aaagaatttc
      121 aggactgcca ccggcaggat cgccgcgtag agcagttcca gcatgctcac cagctggtag
      181 ctggccccga accggatcag ctccttcacc accgaccgcg tcaccaaagt ggaactgatg
      241 cggacctcgg gcaggatgcg gcgcgcgtag atgtaccaca ggatcccgcc cgcaagctga
      301 tacgcgcaga aaaccgccgc aatcgccagc agcccgaagc cggaataaag aaggccgatg
      361 gtaaacgctg cgttcagcag atcgagaaca acattcagct tctcccgcag gtcaatccgg
      421 tgggcgccca tcatgatgct ctgataggtc tggctcacgt tggcgatgat ggccgtgatc
      481 gccaggattc gaatcgcctg ggaagccgga atcagaaagc cggcaggaac gccaaccgca
      541 actgccagcc agtgagaaaa aatcgccagc ggcacgagga ccacaaccga aagcgccagg
      601 accgctgccg tgcccgtgct gagcaggcga ctcgcgcgat cgtagtcgcc ggtgcccgtt
      661 gcctccgcca catacttctg gaacgcactc ttaacgccgg ccccgcccag cgtaatgtag
      721 gcggcaatcg acatcagcac cgcccagatg ccgtagccgt ccagccccag gtgccggatc
      781 atcaccggaa ccacaacgat ccgggccagc acatgcacaa gattgcccac cacgccgaag
      841 agcgtgtttt tcccgatcga agccgtgagc gcccggccgg aagtgggcgc agtgaactta
      901 gcggtcatgg tgtaccggag tggaaatcac tggttattca gaaatggtct gttcgcgttg
      961 atgacgatca gtgcccttcc ggcccctggg cagccggact gggcgtcgac caggcgccca
     1021 ccgtccaccg gtcggcggcg gagcgtggat tcccggcata gtcggaacac aggagcgaaa
     1081 gcgtatcgga acacagccca ttcagcggcg ttccggcatg catcagcggg gatcctgttt
     1141 ccggctgata gctgctgttc accttgagag acgaacggta gtgcgaatgc gaatcgctcc
     1201 ggatacaact ctgccacgaa gaaaactcag catcctgata gaactttccc tggcagacga
     1261 aggcgttatt tccgccgtcg gcatactggt tgtagtccgc gctgaacgga atgctgtcgg
     1321 tctctatcag ctggttgcag ccgctcaccg cgttgttcac aaacgtcatg ccgctcacaa
     1381 tgctgttcgc tgagaagcaa acgcctgccg ctgttcctgc cgtgctgatc agcgtgttgt
     1441 tgtaaactcc gccctgatgc tgcgacgtga agtttccgga aaacagcccg aacaatccat
     1501 ccggatcagg ctgatcccca accgctacgt tgttgtaaat cgcaatgccc gagctggcgt
     1561 ccgcacatgg agtggcgccg gaaccccggc cgccttcaat aaaaatgtgc gccgtgatgt
     1621 tttgcccgat cggccctttg aagaagttgt cgtaaatcgc cagcgacgtg atatgcgcgc
     1681 ccgctccgtg cgtctggctc gtgaaacagt gaatgccgtc gtggtgataa acgtccgcgg
     1741 tacccgtgtc ccacgctgcg tatccgtaga ccgtgttgtg gtgaaacagg aacggtcctg
     1801 aacttcctcc cgctacctga ctggccagca tccagccgtg atcgacgttg taaatcgtat
     1861 tgccggcgat ctccacatgg ccttcgccgt tctggaaatt gttgtacagg caccagcccg
     1921 catcgtgcat cacattattg cggatcagcc agttcgatcc gctgatcgag atgcagcgct
     1981 gcgccgatgc gtccgctgac ttgtcgctga ccgaagtgtg cacatacatg ttctcaatcg
     2041 tcaggttttc aaccgtgcag tgattgcagt cgaatccctc gatcgcctcg gtcttctcgt
     2101 tgtactgcag tcccgtgccg ttcgccgtgt tctcgatgat cccggtcttc tgcccgtcaa
     2161 ccgtgtaata gctcaaaccg tagagacaaa tcgctccgcc gcagcccccg ttcggcgacg
     2221 cggcaaagta gggagcctca acaacggcgc cgctgtcgaa ccggatcgtg accggattcc
     2281 ccgctgatcc atcgcccttc ggagtcagca gttgcgcacc ggcctcgccg gtaagggtcc
     2341 cgcagaggta aatcacatcg cccggcgaat tcgtaatgcg attgaaggtc gccggcgtga
     2401 tggtcgtctg gccgctgcag gctgtcccgc cgctgaaaac tgtttcagtc tgggcaatgt
     2461 agcgagtacc ggcgaacgcc gggcgggtca tgatgaaggc gcccaccacg acaaaggcca
     2521 cagcagtggc gaaaagggga atcagccggt tcacagacgt tcaggatact cttccgccgg
     2581 gccggatcag ggccggcccg ccacggaagc ggaggtgcag aaggatctgg cggcgattgc
     2641 atcgcgcgaa cgcctgcaac gccgccagga gatggtgaag tttagttttg aaccacggtg
     2701 cccgtcagac ctgtcggagg cgctggtgcc gtccccgcgg tcgaataggc gcccaccgtc
     2761 cacgaaccgg tcgcagcgcg cggattgccg ataatgtcgc tgttcaggct caggatgctg
     2821 agggaggtca ggttcgaccc ggcaccgatg accggcgatc cggcctgcgg tattccggcg
     2881 ggtgagaggt tcgcgctgga gacatacgcc gacggactcg catcctgtcc ggtcgcattc
     2941 tgccatgatg cgaacgaccc ggagtaactg ccgttatagg caaagcaatt gctccccgaa
     3001 caccctgcat agatgttgtt gtgcagaccg cccgaggcga agctcccgcc gttggcgata
     3061 tacatcagcg tgccgcaacc gctgaccacg ttgttcacga acgtcacgtt gggagaactc
     3121 gacgtgccca tgcagatgtc gcccgagctg cccatgaacg tgttgttgta gatcttgtat
     3181 ccgccgtcga tccccgtcgt gagaagaccg tcgttgtcgg ttcctgcgca ggtgtcgatc
     3241 agagagttgt tgaagatcga gacgttcttc gtattgccgc tgttggcttc gatgaagatg
     3301 tgcgccgtga cgttcttgcc gatgcagccg ccgaacttgt tgttgtagat gttcaccgcg
     3361 ctgatgctgt cggagccgtc gttgttgtag ccccagatgt gaatcccatc gtggtgccag
     3421 acatcgcccg gcgtgtccca gttcgagtag tcgtggatgc tgttcccgac gatgttgacg
     3481 ttggtcagcg tgttccccga gtgcggtccg ccaaacgcga ttccgtgatc gatgttgtag
     3541 atctcgttgt tcgagaccgt gatgcccgaa gacgtcccgc tgtcgtactg cagattgatg
     3601 caccagctca cgtcatgcat cacgttattg tcgatcgtcc agttgttcac ctggctgtgc
     3661 gcccagatgc aggagccgcc gccgccgctc ggtacgcccg tcgtggtgac gtacatgtcg
     3721 ctgatggtca ggcccttcac gaccagattg ctgctgctgt cggcttcaat cgcgttgctg
     3781 tcgtcgtggt aggtgcatga cccgctcgga cacgctgctc ctgaatcgcc cgtcgccgtc
     3841 gcctggatca atcctcgcgt cgccccgttc gcgccgccgt ttccgtcgat cgtcacatag
     3901 ctctggttgc tggtgttgat cgctccattc gagccctgcg gcgggaattc cggagcctgc
     3961 acaaccgcac cgctctcgaa atacagcgtt accggattgc ctgccgtgcc gctgccctga
     4021 aacgtcagcg cgttctgatt ggcgccaacc gtgatcgttc cacagatgtg aacgacgctt
     4081 cccggaccaa ttttcgttcc cgtaggcgcg gccgacgtcc agttcccact ggtgttgaaa
     4141 tagttgtaag catacgcatt cgcgcaactc gagccgttgc cggaacctgc cgccgcctga
     4201 gcgatataaa tgtcattcgc cctcgcggca accgtacaaa tgccaaacaa aaatgccgct
     4261 gtaaacgcag cactcaatac acgcgcattc ctcaccatga acgaaacctc caggggtcgc
     4321 accgaaccat aacgatctat gcgggacggg tctgcgtgat caatagcaaa ctgaggacca
     4381 gtctggaccg gaaagtgacg ttcgatatgc cgttcggacg atgagcgtgc actaacgtaa
     4441 tctgtcttcc ctatctatct gattatacag cttgttatcg gctagcaaga ccctatctca
     4501 ggccggtcct gctggtagaa ctgccccgaa aaatgactcg tatgcgccga ttctgccacg
     4561 caatccctcg gaggttgcgt aaaaaagtgt tgcctgtggt tacttttggg tgcgtgcttt
     4621 cacgcggggt ttccgcagcc gcttcgccac cgactgcaac cggcccaccc agtcgtaggc
     4681 gcccgctaac ttggcctgca acaggggccc gtcatccgcg ctgttgactg gaagccgttc
     4741 cagaaacaag ggatcgctcg ccgctcccgc tcgcccaatc tgcgtgcaca ccccgtagcg
     4801 gtagccgctc tcttccagaa tcgaccgcag catcgctttg aatgccccat ccgtctgcgg
     4861 gaatgcataa ggataggcaa acgtatcgac cgctacccct gtcttctcct caatcgtctc
     4921 tcgtgactgc aggatttccg cgcgaatccg ctgcggctcc agcccgtgca gctgcggatg
     4981 gttcactgtg tgcgatccga aggagatgcc gcgctgctgc aaatcgcgca cctcgctcca
     5041 ggtcatgcac tcgcgccggt tgaacacctg cggccgctcc ccgatcgatg cggtgggcag
     5101 gaacatcgtc gcggtaaaac cataccgctc cagcgccgga aacgcgtgct ggcagaagtt
     5161 ccgatagcca tcgtcgaacg tgatgaccac cgtcttttcc gcggttccct tacccgctcg
     5221 cagcgactcg accgcatcgc caaggctggt cgtgcgatag ccctgctcat gcagcaacgt
     5281 catctgctgc aggaaccgtg ccggcgtcgt cgcgatccag aagtacggtt gcagagccga
     5341 ttcgtcttcc tcggccacgc tgtggtacat caggatcggg attctgccgt cgccccgccc
     5401 cgccaatacc ttgcggaccg gcgcggccag atacaacgtg ccggcgcgat cgaaccgggg
     5461 gctcaccgct gtttccccgg acgcgatccg ccggcctgaa ccgtgctcgg catcccttag
     5521 ctccgcgtcg ccagaaccct cggctcggcc gcgctccgcc gtggcgccgt gaagttctca
     5581 atttcgtgag ccacgcgtct ctgctgctct gccgtcagct gcgggaacat cggaagcgac
     5641 agaatctctt tcgccagttg cgtggcaacc ggcagatcct ctgccttgta gcccatcgac
     5701 gcgtacgcct tctgctggtg cagcggaacc ggatagtgaa tgcccgtgcc gatgcccgcc
     5761 tctttcaggt gctgcgccag accatcgcga tcggccgtgc ggatcacgta caggtgatac
     5821 accgaacgcg accaggacgg ctcatacggg cacacaaccg cgtcgttgcc cgccagcagg
     5881 cggtcatatt ctgccgcacg atcccgacgc gccgcgttcc acttcgccag atgacccagc
     5941 ttcacctgca gcagtccgca ctgaatcgag tccagccggc cgttgtagcc ttccatgtcg
     6001 tggtagtact tcttcgcctg gccgtgctcg cggatcatct tgatcttcgc cgccaccgca
     6061 gcatcgttgg tcgatacagc cccaccttcg cccagcgcgc caagattctt gcccgggtag
     6121 aagctgaatg ccgcggcatg acccatcgtg cccgccttca tccagcgatt cagcttgccg
     6181 gagaagtact ccgcaccgtg cgcctggcag gcatcttcga tcacc
//
LOCUS       DASZYN010000453         7237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_82827, whole genome shotgun sequence.
ACCESSION   DASZYN010000453 DASZYN010000000
VERSION     DASZYN010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7237
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82827"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VHE33_11700"
     CDS             complement(<1..227)
                     /locus_tag="VHE33_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98161.1"
                     /translation="MDHYVERVVDLVEAGSNHLYNMSVEDARRILLEGPADAVRAIQG
                     SFALTAKSGKMVRMARSLDRPMRYFLAKRQEG"
     gene            complement(289..1050)
                     /locus_tag="VHE33_11705"
     CDS             complement(289..1050)
                     /locus_tag="VHE33_11705"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HVT98162.1"
                     /translation="MSFSTDFASIANILVESLHLKQPPVAISFSESLPAGVPTHSGRV
                     AAGCRFWEDGAAGTFATTAADHSSCAIGIHTHNLQPSPEQQTDLIDALKVFADLGYVR
                     PEDVPQIPVLQSRPKYVVYGPLAQARVAPEVVMLFVNSNQTLILSEATQQVENQVAPA
                     MGRPACAVVPQVMNTGRAALSLGCCGARAYLDVLSDDVALFAIPGAKLVAYAERIATL
                     SKANGILSQFHQIRRRSVESGESPTVKESLAAMSA"
     gene            complement(1078..1404)
                     /locus_tag="VHE33_11710"
     CDS             complement(1078..1404)
                     /locus_tag="VHE33_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HVT98163.1"
                     /translation="MQRRRFVQMMTLAGVGTVAAAELAHSGETRTAAYRVKGFTCITC
                     AVGLETMLREQKGVKWVKATYPEAKVTIRYDPSSVQEGQLRGFISELGFTAEPEAQSQ
                     DSSRNS"
     gene            complement(1448..2503)
                     /locus_tag="VHE33_11715"
     CDS             complement(1448..2503)
                     /locus_tag="VHE33_11715"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVT98164.1"
                     /translation="MSPKKAVVICVSWLVLITGLHAWLNVNWDVVLNDFRPPAQRKMV
                     VAYIPVTCQLTCPVTDYISKFSENGELFLPRMFQGFPEIKEALISNKVQAAFIVAPLA
                     IALASQGVPIKVVYLGHRYGSALVVKKNGPVKNFLDLKGRTVAIPSRFSDERLILFRA
                     MKVWGMKPGDIRMVEMAPPDVSGALAAGAIDGFVMGEPFPSQAEMGGYGRVLFQAREY
                     WPDYMSCILVVRQDLIDQRPEAVQVLVDGIARSGLWLDKGRRYREDAADFVGRYYYNQ
                     KPALLRWALTKPMDRVIYSELEPRKGDFDMVRDLMIETGVLNKKIEFSQYTDTRFSDN
                     AAIQTAWKYDPGLAAAN"
     gene            complement(2493..3275)
                     /locus_tag="VHE33_11720"
     CDS             complement(2493..3275)
                     /locus_tag="VHE33_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT98165.1"
                     /translation="MNALSQPAKWERFFWPVVATGLMLALWYYSVRWSGTKVFPSPRE
                     VEKGLAELLGHHVLWKDIIDSLRRVAVGFGLAVLVGIPLGLTLGWYPAANQVVNPTMQ
                     ILRPISPIAWIPVAIIFFGVGDEAAIFLIFLGAFFPIVVACVNGVANVPSVYRRAGRN
                     FGLTPPQLLARVLFPAALPQILVGLRIALGIAWLVVVAAEMIAVDSGLGYLVIDSRNS
                     GKRYDLVVAAILLIGLIGLILDLAFRRLEKLRSVRWGFRHES"
     gene            complement(3272..4057)
                     /locus_tag="VHE33_11725"
     CDS             complement(3272..4057)
                     /locus_tag="VHE33_11725"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT98166.1"
                     /translation="MQPIAAPLPFTVKTRLSVDRVSMVFERDGKRTEVLNNISLEVGD
                     GEFVCLLGPSGCGKSTLLNAMAGFLTPTSGEIRVDGETVRKPDPRRIFVFQERGVFPW
                     LTVEGNIGFGLSKLGRSEREQRIAHYVKMVGLEGFEKTYPQELSGGMKQRLEVARALA
                     VNPDMLLLDEPFGALDSITRLVMRGELLRIWEAERKTVIFVTHDIDEAVQLADRVVVM
                     SARPATIQQIVNIDIPHPRDISSARYLELRDGIFKQIGLAHKV"
     gene            complement(4066..4824)
                     /locus_tag="VHE33_11730"
     CDS             complement(4066..4824)
                     /locus_tag="VHE33_11730"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT98167.1"
                     /translation="MKLRLLWRPLAFIALLLLLWEVGVRRQASHLLPGPWGVVGGIVD
                     LVRHGLLTKYVVASLFRVTWGFALAVVTAIPLGLAIGWYRRAEMALNPILQILRPISP
                     LAWIPIAILWFGVGDLAAVFLIFVGCFFPLMLTAIDAVQSIPAVYINAGRNFGLSSAS
                     LVYRVLYPAVVPNLIVGLRITLGIAWLVVVAAEMIAVSSGLGFLIVDARNAGNRYDLV
                     VAGMLIIGIIGLLLDQGMRSLEKVKSFRWGYSED"
     gene            complement(5041..5370)
                     /locus_tag="VHE33_11735"
     CDS             complement(5041..5370)
                     /locus_tag="VHE33_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98168.1"
                     /translation="MKVKLCDVNAAAAEKLPGHSDDKSGIGVHYVDAYIKPMNVKLED
                     GTPVRCKRRGLKITLSAGAKKGEGLMRRLDVSKDPVVMLDAALQEAAKAAGIELTVED
                     GAVFVSL"
     gene            complement(5367..6302)
                     /locus_tag="VHE33_11740"
     CDS             complement(5367..6302)
                     /locus_tag="VHE33_11740"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HVT98169.1"
                     /translation="MKKLLQEAILERPLLGDGAMGTQLMLAGLEQGNCGEAWNLTHAE
                     RVLGIQRRYVEAGSECLITNTFGGSRIMLTRHGEGDHVVAINKAGVEIARKAFGDRDG
                     YVIGDIGPFGGLMEPYGDFTEAQVRSAFEEQARALVEGGADAIIIETQTSLEELGIGI
                     ESAQKAGARCIIGSMAYDVTLDGSTFRTMMGIEPEKAAEFMEQRGAHIVALNCGTRMD
                     MTRALTAVERYKSVTSLPVMVQPNAGQPKLVQMKVVYDETPDEMVKDLLPILKAGANI
                     IGACCGSTPEHIRAFRKTMDQYLESKGIALERSRA"
     gene            complement(6305..>7237)
                     /locus_tag="VHE33_11745"
     CDS             complement(6305..>7237)
                     /locus_tag="VHE33_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98170.1"
                     /translation="PEAEAEARRLWSEEKPDEYFFLEMFASAVVEHLTTTAGARLCDW
                     AEQHGMAVLPHYSPGYPEWDVAEQLRLLEVIKRTRSAEFPSQLEALESGMLRPRKSQL
                     AVFGLTRHVDRLRRLTSLVPCENCSFGPCQYRRAAYRKPPRGCGERVGSAPALEPDAA
                     YNTHRRALQRWAQERLTIEEHEDGSLDAMFRYDGTTCTNMGRPLVFQYRVKLGPREDG
                     YPIREQLCAPAEGDTGHTHMCEYIADPEGVMGAIDREKPLNGERLNAVLSWRREASGA
                     GCFCEAASRNHKWGLVLETIHYALAQRERAQETE"
BASE COUNT         1519 a         2592 c         1787 g         1339 t
ORIGIN      
        1 ccctcctgac gcttggccag aaaataccgc atcggccgat ccagcgatcg cgccattcgc
       61 accatctttc ccgacttcgc cgtcagggca aaggatccct gaatggcacg caccgcatcg
      121 gccggcccct ccagcaaaat cctgcgcgcg tcctcgacgc tcatgttgta aagatgattc
      181 gatccggcct caaccagatc gacaactcgt tcgacgtaat gatccatcgg cgcctcgaaa
      241 gcagtgtacg cggagcagct caggaaattc cccgtccccc gggccgtcct aagcgctcat
      301 cgccgccagc gattctttca ccgtcgggct ctctcctgat tcgactgagc gccggcggat
      361 ctggtggaat tgcgacaaaa taccgtttgc cttcgacaga gtcgcaatcc gttccgcata
      421 cgccacgagc ttcgctcctg gaatcgcgaa gagcgccaca tcgtccgaca gtacatccag
      481 gtaggcacgc gcgccgcaac aacccaggct caatgccgcg cggcctgtgt tcatcacttg
      541 cggaacaacg gcgcacgccg gccttcccat cgcaggcgcg acctgatttt ctacttgctg
      601 tgtcgcttcg gaaagaatga gcgtctgatt cgagttcacg aacagcatca caacctccgg
      661 cgcgacccga gcctgcgcca gcggaccgta caccacgtac tttggccgcg actgcagcac
      721 cggaatctgc ggaacatcct ctggccgcac gtaacccaga tcggcaaaca ccttcagagc
      781 gtctatcagg tcggtctgct gttccggcga cggctgcagg ttgtgcgtgt gtatgccgat
      841 cgcgcagctg gaatgatcgg ccgccgtcgt tgcgaaggtt cccgccgctc cgtcctccca
      901 gaatcgacat ccggcggcaa cccggccaga atgggtcggc acgcccgccg gcagcgattc
      961 ggaaaacgag atggccaccg gcggctgctt caaatgcagc gactccacca ggatgttggc
     1021 gattgatgcg aaatccgtag aaaaactcat gacactcctt gcagatcgaa cctctcctca
     1081 cgaattccgg gaggagtcct ggctctgtgc ttcgggctca gcagtaaaac ccagttcaga
     1141 aatgaatccg cgcaactggc cttcctgaac cgaagacgga tcgtagcgga tcgtcacctt
     1201 cgcctcaggg taagtggcct tcacccactt cacgcccttt tgctcgcgca gcatggtttc
     1261 caaaccgacc gcgcacgtaa tgcaggtgaa ccccttcacg cgatacgcgg cggtcctggt
     1321 ctctcccgag tgggccaact ctgccgcagc caccgttcca acacccgcca gcgtcatcat
     1381 ctgcacaaat cttctgcgtt gcatatcctg tcctcctgtc cggccggcca gaaccagacc
     1441 gcaatgttca gttcgcagca gccaggcccg gatcgtattt ccacgccgtc tgaatcgcgg
     1501 cattgtcaga gaatcgggta tcggtgtact ggctgaactc aatcttcttg ttgagcactc
     1561 cggtttcgat catcaaatcg cgaaccatat cgaaatcacc cttgcgcggt tccagctcgc
     1621 tgtagatcac ccgatccatt ggcttcgtca gcgcccatcg cagcagcgcc ggcttttggt
     1681 tgtagtaata gcggcccaca aaatccgccg catcctcgcg gtatctgcgg cccttatcga
     1741 gccacagtcc cgagcgcgca atcccgtcca ccagcacctg cacagcttca ggacgctgat
     1801 cgatcaggtc ctgccgaacc accaggatgc acgacatgta gtccggccag tattcgcgcg
     1861 cctgaaacag aacgcgtccg tagccgccca tctccgcttg tgaaggaaac ggctcgccca
     1921 tcacgaagcc gtcgatcgcg cccgccgcca gtgctcccga cacatccggc ggagccatct
     1981 cgaccatcct gatatcgccc ggcttcatcc cccacacctt catggcgcga aacaggatca
     2041 gccgctcatc ggaaaaacgg ctgggtatcg caaccgtacg gcccttcaga tcgagaaagt
     2101 tcttcaccgg gccattcttc ttcaccacca gcgcgctgcc gtaacgatgt cccagataca
     2161 ccaccttgat cggcacgccc tgcgaggcca gcgcaatcgc cagcggcgca acgataaacg
     2221 ccgcctgcac cttgttcgaa atcagcgcct ctttaatttc cggaaagccc tggaacatgc
     2281 gcggcaaaaa aagctccccg ttctccgaaa acttcgagat gtagtccgtc accggacacg
     2341 tcagctggca ggtcaccgga atgtacgcca ccaccatctt tctttgcgcc ggcggacgaa
     2401 aatcgttcag caccacatcc cagttcacgt tgagccacgc gtgcagtccg gtgatcagca
     2461 ccagccacga gacgcaaatc accaccgcct tcttaggact catggcggaa cccccatcgc
     2521 acggaacgca gcttttccaa acgccggaaa gccaggtcaa gaatcaagcc gatcaggcca
     2581 atcagcagaa tggcagccac caccaggtca tagcgcttgc ccgagttgcg ggagtcgatc
     2641 accagatatc caagccccga atccaccgcg atcatctccg ccgccaccac caccagccac
     2701 gcaatcccca aagcgatgcg aagccctaca agaatctgcg gcagcgccgc gggaaacagg
     2761 acccgagcca gcagttgcgg cggtgttaag ccaaaattgc gtccggcgcg ccggtacacc
     2821 gacggcacat tcgccacccc attcacgcag gccaccacaa taggaaaaaa cgcccccaga
     2881 aaaatcagga aaatcgccgc ctcgtcgcca actccaaaga aaataatcgc gaccgggatc
     2941 cacgcgatgg gactgatcgg tcgcagaatc tgcatcgttg gattcaccac ctgattcgct
     3001 gccggatacc agcccagcgt cagtcccaga ggaatgccaa ccaggaccgc cagtccaaaa
     3061 ccaacagcaa cgcgccgcag ggaatcgatg atgtccttcc acaaaacatg gtgacccagc
     3121 agctctgcca gtcctttttc cacctcgcgg ggcgacggaa aaaccttcgt tccgctccag
     3181 cgcacggaat agtaccagag cgcgagcatg agccccgtgg ccaccacggg ccagaagaac
     3241 cgctcccact tcgcgggttg gctaagcgcg ttcatacctt gtgcgccagg ccaatctgtt
     3301 tgaaaatgcc gtcgcgcagc tcgagataac gcgcagagct gatgtcccgc ggatggggaa
     3361 tatcgatgtt cacaatctgc tggatcgttg ccggccgtgc gctcatgacc acaacccgat
     3421 cggccagctg caccgcctcg tcgatatcgt gcgtcacaaa gatgaccgtc ttgcgctcgg
     3481 cctcccagat gcgcagcagc tcgccgcgca tcaccagtcg cgtgatcgaa tccagcgcgc
     3541 cgaacggttc atccagcagc agcatgtcgg gattcacggc caacgcccgc gccacttcca
     3601 ggcgctgctt catgccgccg gaaagctcct gcgggtaggt cttctcgaac ccctcgaggc
     3661 ctaccatctt cacgtaatgc gcaatccgct gttcgcgctc gctcctcccc agcttcgaca
     3721 acccgaaacc aatgttgcct tccaccgtta gccacgggaa cacgccccgc tcctgaaaca
     3781 caaagatgcg tcgcggatcc ggcttgcgta ccgtttcacc gtccacccgg atctccccgc
     3841 tggtgggcgt caaaaatcca gccatagcat tgagcaacgt cgatttccca caacccgacg
     3901 gccccagcag gcagacgaat tccccatcgc ccacttccag actgatgttg ttcagaacct
     3961 cggtccgctt gccgtcacgc tcgaagacca tactcactcg atcgacactc agcctggtct
     4021 tgacggtgaa cggcaaaggg gcggctatag gttgcatcac gggcatcaat cctccgagta
     4081 tccccagcga aatgacttca ctttttcaag cgaccgcatc ccctgatcaa gcagcagtcc
     4141 gatgatcccg ataatcagca tccccgcaac caccaggtcg tagcgattac ccgcgttcct
     4201 cgcgtcaaca atcagaaacc ccagccccga actcaccgca atcatctccg ccgccaccac
     4261 caccagccac gcgatcccca gcgtgatccg cagccccaca atcaggttcg gcaccaccgc
     4321 cggatacagc acgcgataca ccaggctcgc ggaactcagt ccaaaattgc ggcccgcgtt
     4381 gatgtaaacc gccggaatac tctgcaccgc atcgatcgcc gtcagcatca gcgggaagaa
     4441 gcagccgaca aaaattaaga agaccgccgc aagatcccca accccgaacc acaaaatcgc
     4501 aatgggaatc catgccagcg gcgaaatcgg acgcaggatc tgaagaatcg gattcagcgc
     4561 catctccgcg cgccgatacc agccgatcgc cagccccagc ggaatcgccg tcaccaccgc
     4621 cagagcgaag ccccacgtca cccggaacag cgacgcaacc acatatttcg tcagcagccc
     4681 atggcgcacc agatcgacga tcccccccac cacaccccag gggcctggca gcagatgact
     4741 tgcctggcgg cgaaccccca cctcccacaa caacagcaac aaggcgatga acgccagcgg
     4801 ccgccatagc aggcgaagct tcatcgatgt cgagtgttcc tctcgcatga aagggtcatg
     4861 atcctgcggc gcaaacaccg cgccgattgc cgcaacgtca gcgttgagat tttcagacct
     4921 ggggtaactg ggaactagtt tgcgcgttta tgctacccgc ccgaatacgg tccgcgcctc
     4981 atccgaaaga tggatactgc ccaaagacac tacgggcctg tctgacagct tttccgcgag
     5041 tcagagcgaa acgaagaccg ctccgtcctc cactgtcaat tcaatgcctg ccgccttcgc
     5101 tgcttcctgc agcgccgcat ccagcatcac caccgggtct ttgctcacgt ccagtcgccg
     5161 catcagccct tcgcccttct tcgcccctgc cgacagtgtg atcttcagcc cccgccgttt
     5221 gcaccgaacc ggcgtaccgt cttccagctt cacattcatc ggcttgatat aggcatcgac
     5281 atagtgcacg ccgattccgg atttatcgtc actatgccca ggcagctttt cggctgcagc
     5341 tgcattcaca tcgcaaagtt tcactttcac gctcggctcc gctccagggc gatccctttt
     5401 gattccagat attgatccat cgtcttgcga aatgcgcgaa tgtgttccgg cgtgctgccg
     5461 cagcaggcgc cgatgatgtt cgcacctgct ttgaggatcg gcagcaggtc tttcaccatc
     5521 tcatccggcg tctcgtcgta caccaccttc atctgcacca gcttcggctg tcccgcattc
     5581 ggctgcacca tcaccggcag gctggttacg gatttataac gctcgaccgc cgtcagcgcg
     5641 cgcgtcatgt ccatcctcgt gccgcagttc agcgccacaa tatgcgcccc gcgctgctcc
     5701 ataaattcgg ccgccttctc cggctcgatc cccatcatgg tgcgaaacgt cgatccgtcc
     5761 agcgtcacgt catacgccat cgagccaatg atgcaccgcg cgcccgcctt ctgcgccgac
     5821 tcgatcccga tccccagctc ttccagcgaa gtctgcgtct cgatgatgat cgcgtccgcc
     5881 ccgccttcga ccagcgctcg cgcctgttcc tcaaacgcac tgcgcacctg ggcttccgtg
     5941 aagtcgccgt agggctccat caatcctccg aagggcccaa tgtctccgat aacgtaaccg
     6001 tcgcggtcgc caaacgcctt ccgagcgatc tccacaccag ccttgttgat cgccaccacg
     6061 tgatcgccct caccatggcg cgtgagcatg atgcgcgaac ccccaaacgt attcgtgatc
     6121 aggcattcgg atcccgcctc gacataccgc cgctggatcc ccagcacccg ctccgcatgc
     6181 gtcaggttcc acgcctcgcc gcaattgccc tgctccagcc cggccagcat cagttgtgtt
     6241 cccatcgcgc cgtcgcccag cagcggccgc tccagaattg cttcctgcaa aagtttcttc
     6301 atcgtcattc cgtttcctgt gcccgctctc tctgtgccag cgcgtagtga atcgtctcca
     6361 gaaccagtcc ccacttgtga ttacggctcg ctgcttcgca aaaacagcct gccccgctcg
     6421 cttcgcgtcg ccacgacaac accgcattca ggcgctctcc gttgagcggc ttttcccgat
     6481 caatcgcgcc catcacgccc tccggatcgg cgatgtactc gcacatatgc gtgtgacccg
     6541 tatccccctc cgccggcgcg cacagctgtt cgcggatcgg atacccgtcc tcgcgcggac
     6601 ccagcttgac tcgatactga aacacgaggg gccggcccat gttcgtacag gtcgtcccgt
     6661 cgtagcgaaa catagcatcg agcgaaccat cctcatgctc ctcgatggtc agccgctcct
     6721 gcgcccagcg ctgcagagcc ctgcggtgcg tgttgtaggc ggcgtccggc tccagcgccg
     6781 gcgcacttcc cacccgctca ccgcatcccc gcggcggctt gcgatacgcc gcccgccgat
     6841 attggcacgg cccaaaagag caattctcgc agggaaccag cgacgtcagc cggcgcagcc
     6901 ggtccacgtg ccgcgtcaat ccaaatacag ccaactgcga ttttcgcggc cgcagcatgc
     6961 cggattccag cgcctccagc tgcgacggaa actccgcgct ccgcgtccgc ttgatcacct
     7021 ccagcaaacg taactgctcg gcgacatccc attccggata gccaggactg tagtgcggca
     7081 gcaccgccat gccatgctgc tccgcccagt cgcacagtcg cgcacccgcc gtcgtcgtca
     7141 gatgctccac caccgccgac gcgaacattt ccaggaagaa atattcatcg ggcttttctt
     7201 ccgaccacag gcgcctcgct tctgcttccg cctccgg
//
LOCUS       DASZYN010000454         1992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_82888, whole genome shotgun sequence.
ACCESSION   DASZYN010000454 DASZYN010000000
VERSION     DASZYN010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82888"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VHE33_11750"
     CDS             <1..560
                     /locus_tag="VHE33_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98171.1"
                     /translation="FLIAGVLTGAAVLRAGFRIFVGLGDSGPVDESAKVDEGPETREE
                     QNIYWFLFVPALVCLGSAVALAFWPAFANGVHSCAIRFMDFRSYAHAVYQRATPQSLL
                     AAPQLHGGLAVLLAVLRPLLAAGIALWAVYRIRVPRALRWPSHLEGPLRPLRELQSGH
                     PGDYVAWLTVGFAALGGVAFWLGLR"
     gene            complement(625..816)
                     /locus_tag="VHE33_11755"
     CDS             complement(625..816)
                     /locus_tag="VHE33_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98172.1"
                     /translation="MAFAEGQQVRVKQGQAIGVVDRKLAYLQDMYVVLLSGNQTPAKK
                     LAHESDLELLRQADERQSA"
     gene            1018..>1992
                     /locus_tag="VHE33_11760"
     CDS             1018..>1992
                     /locus_tag="VHE33_11760"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HVT98173.1"
                     /translation="MTSTIEIPAAKPPLLPISAIAHKLGLRESDYDPLGPWFAKLHLD
                     LLRDPSRPPHGKLILVAATTPTTSGEGKTVTSIGLVQGLERIGKKAIITSREPSLGPV
                     FGMKGGAAGGGLSQIEPSQKVNLHFTGDFHAITSAHNLLAALIDAHLFHGNDLDIDPA
                     SISWPRAMDMNDRALRRILIEIDGKRDGASRRTGFVITAASEIMAILALARDRNDLRR
                     RLARIVIGATRNGKPVTADDLHATGAMMALLADAIEPNLVQTSEGTPALVHAGPFGNI
                     AHGTSSIIAQQIGLRLADYVVNEAGFAADLGAEKYIDIVMGASGSSPSA"
BASE COUNT          323 a          703 c          561 g          405 t
ORIGIN      
        1 tctttctcat cgccggcgtc ctcaccggcg ctgccgttct gcgcgctgga ttccgtattt
       61 ttgtgggcct gggcgactcc gggccggtcg acgaatccgc gaaagtcgac gaagggccag
      121 aaaccaggga agagcagaat atttactggt ttttgtttgt cccggcgctg gtctgtctcg
      181 gctcggcggt tgcgctggcg ttctggccgg cgttcgccaa tggggtgcat tcctgcgcaa
      241 ttcggttcat ggattttcgc agctatgccc atgcggtgta tcagcgggcc acacctcagt
      301 ctctgctggc cgctccccag cttcatgggg ggcttgcagt tctgctcgcg gtcttacgcc
      361 ctctgctcgc ggccggtatc gctctctggg ctgtctatcg tattcgggtg cctcgggcgc
      421 tgcgctggcc ttctcacctg gagggtccgc tgcgtcccct ccgtgaactg cagagtggac
      481 acccgggcga ctatgtcgcc tggctcacgg ttgggtttgc tgctcttggg ggggtcgctt
      541 tctggctggg tcttcgataa agaagaacaa tcgcgcgtcg gtcgtcccga tctgggcgct
      601 gaacgccgat aactgaaggc ggtcttacgc gctctggcgc tcgtcggcct gccgcagcag
      661 ttccagatcc gattcgtgcg ccagtttttt ggccggtgtc tggttgccgc tgagcagcac
      721 cacgtacatg tcctggaggt aggcgagctt ccgatcgacc acaccgattg cctgcccttg
      781 ctttacacga acctgctgtc cttctgcgaa tgccatgcgc agaatcttct ccggatttgg
      841 gcggcggaac aatagctcga tggagacacc ctccatccct ccaggggata cgcagcgggg
      901 cattaccaca tgcccctgaa gttgggttac tacggtccta tttccatttt ccgaggggct
      961 taactccctg tgcgaaccgt cacatccctg atcggggcgc aacctcaaca atattccgtg
     1021 acttccacga tcgagattcc cgccgccaaa ccgcctttgc tcccgatcag cgccatcgcc
     1081 cacaagctcg gcctgcgcga aagcgactac gatccgctcg gcccctggtt cgccaagctc
     1141 catctcgatc tgctccgcga tccctctcgc ccgccgcacg gcaagctgat tctggtcgcc
     1201 gccaccactc ccaccacctc cggcgaaggc aaaaccgtca cctccatcgg actcgtgcag
     1261 ggcctcgagc gcattggcaa aaaagcgatc atcacctccc gcgagccctc tctcggcccg
     1321 gtcttcggga tgaaaggcgg cgcagcgggt ggcggtctct cgcagatcga acccagccag
     1381 aaggtcaatc tgcatttcac cggcgacttt cacgccatca cctcggccca caacctgctc
     1441 gccgccctga tcgatgccca tctcttccac ggcaacgatc tcgacatcga ccccgcgagc
     1501 atctcctggc cgcgcgccat ggacatgaac gatcgcgctc tccgccgcat tcttatcgag
     1561 atcgacggca agcgggacgg cgccagtcgc cgcaccggct tcgtcatcac cgcagcctcg
     1621 gagatcatgg ccatcctggc tctggcgcgc gaccgtaacg acctccgccg acgcctcgcc
     1681 cgcatcgtca tcggcgcgac ccgcaatggc aagcccgtca ccgccgacga cctgcacgcc
     1741 acgggcgcca tgatggcgct gctcgccgac gcgatcgagc ccaacctggt ccagaccagc
     1801 gaaggcacgc ccgcgctcgt gcacgccggc cccttcggca acatcgccca cggcacgtcg
     1861 agcatcatcg ctcagcaaat cggcctccgc ctcgccgact acgtcgtcaa cgaagccggc
     1921 tttgccgcgg acctcggggc ggagaagtac atcgacatcg tcatgggcgc ctccggctcg
     1981 agtccttcag ct
//
LOCUS       DASZYN010000455         3843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_922339, whole genome shotgun sequence.
ACCESSION   DASZYN010000455 DASZYN010000000
VERSION     DASZYN010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922339"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2568
                     /locus_tag="VHE33_11765"
     CDS             <1..2568
                     /locus_tag="VHE33_11765"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HVT98174.1"
                     /translation="WQNQVSSPQNQKIENDGNATLTFSAMAPDANAAVASSGTTCATT
                     SSVAVDGSCVVAAEFAPTSATLTNPLVGNVNLTGDTANSPLDISVVGQALGLNGAIVT
                     LTSSLNPAAYGQTVTLNVTVKQDPNSTQGTPTGTVTYSDTFQGVTTTLGTPTLNGSGN
                     ASLQISTLAVGTHVITAHYGGDTYYSASDSNPLSQVVQEQVTVVVTNISGGNPSAYGA
                     MVTFSAAVSVSGGIPVSGAVSFYNDGNYLGSGTLGGNVATYSTATLPVGSNSITASYT
                     DTNNVTGTSSVLTQVVKQNTATALTASVNPVIHGMPVMFTAVVTATGTTAPTGTVTFY
                     DATTQIGTGTLAASGTTTAVATFQTSTLAAGTHSITASYGGDANDFTSTSAALSETVN
                     IATSTTTLAASANPSIAGKALTLTGTVRTNGGTAAGTVNFYNGAALLGSGTLNGAGVA
                     TFTTSTLAVGSYSITANYAGDANDTGSSSAVLALSVIQATTSVRLVASSASVQVTAPV
                     TFTATVTGNGGVPTGSVTFMDGANTLGSVAVNAGGVAAYTTSSLGVGTHTITAVYGGD
                     TKDGGSTSSGVTETVTAYATQTSLVASATSINTDQELTLLTTTTSSSGGAVTGTITYM
                     NGTTTLGTATVGANGAATFTLNPPAASYSITAHYSGDALNAPSVSNAVSVHVAQATEF
                     TIGLNPTSMSLPTTKAGQLTITLGSQNGFSDKMALGCNSLPYSVSCNFSNNDINLSAN
                     GTASVTMTVDTNSPLASGGQAKNEGLFGSGGMLAAWILPGAALFGFAFRRFRKNAAVL
                     RVLMVLAMLAGTTFLMTGCGGFSLSSAKPGNYVIQVTATGTQTGVTHVANLTLTVTQ"
     gene            2586..3140
                     /locus_tag="VHE33_11770"
     CDS             2586..3140
                     /locus_tag="VHE33_11770"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HVT98175.1"
                     /translation="MPRRGIAWSFAAVLLLAGVCSARAQSPESAVGDWSSLAVGGSFN
                     ASRIQYGQRWLLGGTAFVDANFTWRFGIEGETNWEVLRQFEKIHATTYLVGPRYQFSA
                     IGQNAQWRPYVKFLAGDGYFNFPYHYAWGNYFVMAPGGGVDYRVNSRIRLRVVDMEYQ
                     YWPGFTYGSMTTFNVSTGVRYRIR"
     gene            3346..>3843
                     /locus_tag="VHE33_11775"
     CDS             3346..>3843
                     /locus_tag="VHE33_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98176.1"
                     /translation="MSSGVGPFMAGTSQLEMFAAFLREIGAGELAASQDTITGRQQPR
                     VNWTMVNRTTIGLSDGEWKTAYGILIDGSQQVSEWSDQMQDALGWRNGRFEADAAQQA
                     ARKAKFERLSARGDTIVEQTMLRLRRQLGNEAFSKLDTFVSHRESGGRSVDRGPIRKG
                     PVETAQ"
BASE COUNT          780 a         1048 c         1359 g          656 t
ORIGIN      
        1 tggcagaacc aggtgagctc gccgcagaac cagaagattg agaacgacgg caacgcgacg
       61 ctgacgttct cggcgatggc gccggatgca aatgcggcag tggcgtcgag cggaacgaca
      121 tgcgcgacga ccagcagcgt agcggtggat gggagctgcg tggtcgcggc ggagtttgcg
      181 ccaaccagtg cgacgctgac gaatccgctg gtggggaatg tgaacctgac cggggacacg
      241 gcgaattctc ctctggatat ctccgttgtg ggccaggcgc tggggctgaa tggcgcaatc
      301 gtgacgctga catcgagcct gaatcccgca gcctatgggc agaccgtgac gctgaacgtg
      361 acggtgaagc aggatccgaa ctcaacgcag ggaacgccga ccggaaccgt gacttattcg
      421 gatactttcc agggagtaac cacgacgctg ggcactccga cgctgaacgg cagcggcaac
      481 gcgagtctgc aaatcagtac gctggcggtg ggaacgcatg tgatcaccgc gcactacggc
      541 ggcgatacct attacagcgc ttcggattcg aacccgctgt cgcaggtcgt gcaggaacaa
      601 gtcaccgtag tggtgacgaa catcagcggc ggcaatccgt cggcctacgg cgcgatggta
      661 acgttcagcg cggcggtgag cgtttcgggc gggattccgg tttccggcgc cgtgtcgttc
      721 tacaacgatg gaaattatct gggcagcgga acgctgggcg gcaatgttgc gacctattca
      781 accgcaacgc tgccggtggg gagcaattcc atcacggcga gttacaccga cacgaacaat
      841 gtgacgggca cttcttcggt actgacgcag gtggtgaaac agaacacggc gactgcgctg
      901 accgcgagcg tgaacccggt gatccatggc atgcctgtaa tgttcaccgc ggtggtgacg
      961 gcgacgggga cgacggcgcc gacaggcacg gtgacgttct acgacgcgac cacgcagatc
     1021 gggacaggaa ctctggcggc ttcagggacg acgactgcgg ttgcgacctt ccagacttca
     1081 acgctggcag caggaacgca ttccatcacg gcctcgtatg gcggggacgc gaacgacttc
     1141 accagcacgt cagcggcgct gagcgagacg gtgaacatcg ccacttcgac gacgacgctg
     1201 gcggcgagtg cgaatccatc gattgccggg aaggcactca cgctgacggg aacggtgagg
     1261 acgaatggcg gcacggctgc cggcactgtg aatttctaca acggagcagc gctgctggga
     1321 tcgggaacgc tgaatggcgc gggggtggcg acgtttacca cgtcaactct ggcggtaggg
     1381 agctactcga ttacggcgaa ctacgcgggt gatgcaaacg acaccggcag tagttcggcg
     1441 gtgctggcgc tgagcgtgat ccaggcaacc acgagtgtga ggctggtggc gagcagcgcc
     1501 tcggtgcagg tgacggcgcc ggtgaccttc accgcgacgg tgaccggcaa cggcggcgtg
     1561 ccgacgggca gtgtgacgtt catggatggg gccaatacgc tgggctcggt cgcggtgaac
     1621 gcgggcggcg tggccgccta cactacgtcc tcgctggggg tgggaacgca cacgatcacg
     1681 gctgtctacg gcggtgacac gaaggatggg ggaagcacgt catcgggagt cacagagacg
     1741 gtaacggcat acgcgacgca gacatcgctg gtggcttcgg cgaccagcat aaacacggac
     1801 caggaactga ctttgctgac gacaacgaca tcgagcagcg gcggagcggt gacggggacg
     1861 atcacgtaca tgaacggaac cacgaccctg ggaacggcga cggtgggcgc caacggagcg
     1921 gcgaccttca ccctcaatcc accagccgcc tcgtacagca tcacggcgca ctacagcggc
     1981 gatgcactca acgcgccatc ggtttcgaat gcggtgagcg tgcatgtggc gcaggcgacg
     2041 gagttcacca ttgggctgaa tccgacatcg atgagtctcc cgaccacgaa agcggggcaa
     2101 ctgaccatca ctctggggtc gcagaacggc ttctccgaca agatggctct gggatgcaac
     2161 tcgctgccgt attcggtttc atgcaacttt tcgaataacg acatcaacct gagcgcgaac
     2221 ggaacggcgt cggtcacgat gacggtggat acgaattcgc cactggcttc aggaggacag
     2281 gcgaagaacg aaggactgtt cggcagcgga ggcatgctgg cggcgtggat tctgccgggc
     2341 gcggcgctgt tcggattcgc gtttcggcga ttccggaaga atgcggcggt gctccgggtg
     2401 ctgatggtgc tggcgatgct ggccgggacg acattcctga tgacgggctg cggcgggttt
     2461 tctctgagca gcgcgaaacc ggggaactat gtgattcagg taacggcgac cgggacgcag
     2521 accggcgtca cgcacgtggc caatctgacg ctgacggtga cccagtgagc ggagggggca
     2581 acaggatgcc acgcaggggg atcgcctgga gcttcgcggc ggttctgttg ctggccgggg
     2641 tttgcagtgc gcgcgcgcaa tcgccggagt cggccgtggg ggattggtcg tcgctggcgg
     2701 ttggcggcag tttcaacgca tcgcgcatcc agtacgggca gcgctggctg ctgggcggca
     2761 ctgcgttcgt cgatgcgaac ttcacgtggc gatttggaat cgaaggggag acgaactggg
     2821 aggtgctgcg gcagttcgaa aagatacacg cgacgacgta tctggtggga ccgcggtacc
     2881 agttttccgc catagggcag aacgcgcagt ggcggcccta tgtgaagttt ttggcgggcg
     2941 acggatattt caatttccct tatcactacg cgtgggggaa ctattttgtg atggcgccgg
     3001 gcgggggcgt ggactaccgg gtgaactcgc gcatccgtct gcgcgtggtg gatatggagt
     3061 atcagtactg gcctgggttc acgtatgggt cgatgacgac gttcaacgtg agcacgggcg
     3121 tgcggtatcg gattcggtga gaactcgcgc cgtccaacca ggcggccggg aagagaaggg
     3181 ctcgagtcgt gcttctgcaa ggagtgaggt tctatgcctg agttccttcg acgtgtgcag
     3241 ttccttgctg ttctttttgg tgaggtagtt tgcatcctgg gggcaacccc agcccctgcc
     3301 cagaatgtgc ccgcgcctgc accgccacat cccagtgagg cggttatgtc gagcggggtc
     3361 gggccattca tggcggggac gagtcaactg gagatgttcg ctgcattcct ccgagagatt
     3421 ggcgccgggg aactggcggc gagccaggac acgatcacgg gacgccagca gccgcgcgtc
     3481 aactggacga tggtgaaccg gaccacgatc ggcctcagcg acggggagtg gaagacggcc
     3541 tacgggattt tgattgacgg cagccagcag gtgtccgagt ggagcgacca gatgcaggac
     3601 gcgctgggct ggcggaatgg gcggttcgag gcggacgccg cgcaacaggc agcccgaaag
     3661 gccaaattcg aacgattgag tgcgcgcggg gacacgatcg tggaacaaac gatgcttcgg
     3721 cttcgccggc aactgggtaa cgaggctttc agcaagctgg acacattcgt cagccatcgc
     3781 gaaagcggcg ggagaagcgt ggatcgaggg ccaatccgca aaggtccggt ggaaacggcg
     3841 caa
//
LOCUS       DASZYN010000456         9926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1045151, whole genome shotgun sequence.
ACCESSION   DASZYN010000456 DASZYN010000000
VERSION     DASZYN010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 9926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9926
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045151"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VHE33_11780"
     CDS             complement(<1..355)
                     /locus_tag="VHE33_11780"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HVT98177.1"
                     /translation="MPHTRLRVSAIHFLNPAPLMWSFEHEPDRSRLAQRYALTSDTPA
                     ECATKLASGDADIGLVPVSALAFTPSLAVIPGCTIASKDRVRSILLVVRHPDGIAPED
                     MSGVARVALDSSSRTS"
     gene            574..1281
                     /locus_tag="VHE33_11785"
     CDS             574..1281
                     /locus_tag="VHE33_11785"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HVT98178.1"
                     /translation="MASSLVLAYALPENVLQPFLTPGLELDTYRGFGFLAIAMVQTRE
                     LRPAFLPPGFGLNFFLTGYRIFTRYRTLGGRSLRGLRILRSDTDRRSMRTLGNLLTHY
                     GYELSRCRVLQTENSYQVEICTPKGDADLKVVADLATEPQSPPPGSPFADMREARKFA
                     GPLPFTFDYERQTHSVIRVEGVRQAWNPRPVSVEVQRNTFLEREPFRSAKPTLANAFY
                     LENVPYSWKPGIRETLP"
     gene            1278..2048
                     /locus_tag="VHE33_11790"
     CDS             1278..2048
                     /locus_tag="VHE33_11790"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HVT98179.1"
                     /translation="MMSKGASGRFEGVIKIVRFNVRFYVLSGMGIIGVAVLLASRKLP
                     PWLGVILLCGAAVGAFWTLSSLLVSWYVYDHAGVTRWLWLPERIPAPPKVWANIHAGL
                     DESTAVLRNLFPGTEGLVVDIYDPAEMTEPSIARARELHPATEDFRRGRYDALPLSES
                     SCGAVFVLFAAHEVRAPEHRSRLLRETRRVLGEGGYVVLVEHLRDWKNFIAFGPGFLH
                     FHSRRSWLNGIREAGLTIDHAGPVTPFVECFVLRKGRP"
     gene            2045..2647
                     /locus_tag="VHE33_11795"
     CDS             2045..2647
                     /locus_tag="VHE33_11795"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT98180.1"
                     /translation="MKLNFAEQLLVNNPFRTAVQRYYEGPLLRRLGGVVSVGTVLEVG
                     CGRGAGVEVILRQFRATRVFGIDLDPLQIERARKRLQGKYSDRVTVMQGDAEQLSFAN
                     ETFDAVFDFGALHHVPDWQRSISEIRRVLRPGGTFFFEEVTRAALERWIYRRFLDHPG
                     ENRFSEAEFLEELGRQGMEPGTRLRRTLFGDIFIGVAKVS"
     gene            2837..4009
                     /gene="dprA"
                     /locus_tag="VHE33_11800"
     CDS             2837..4009
                     /gene="dprA"
                     /locus_tag="VHE33_11800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HVT98181.1"
                     /translation="MTTATPPSVTSTSFEEKHAWLGLALTPGIGPRRLLRAVERYGSA
                     ARVLHLPLTELEALQLPAESAQFISGGEAAHAADQELEKLAASGASILTWHDEAYPLR
                     LREIYDPPALLWVRGDASLLARPSLAVVGTRHPTPYGTGIAAKLARELAMRGLIILSG
                     MARGVDTAAHKGALEAKRPTIAVWGTGVDVIYPKENKSLAEQIVQGGGLILSELPMGT
                     FPAPQNFPKRNRILSGMSVGVLVIEAAENSGTRVTARCALEQNREVFAVPGNVTTKNA
                     WGPNLLIKQGAKLTSCWEDVWEELPTQIRMELEQSDGFASTAGSEASLFEESPLPPSE
                     ARVLGVLRMDEALQMDEILEKLEPDVSSSEVFTALFELELAGRIRQLPGKNYVRSL"
     gene            4168..7161
                     /gene="topA"
                     /locus_tag="VHE33_11805"
     CDS             4168..7161
                     /gene="topA"
                     /locus_tag="VHE33_11805"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HVT98182.1"
                     /translation="MSKSLVIVESPAKAKTINKYLGKDYEVEASFGHIMDLPKRDIGV
                     ELERRTFEPTLIVSPDKEKVVAKLRKMASKADAVYLAPDPDREGEAIAAHLSLQLMPV
                     VKDKRRIRRVTFNEITQKAVRAAFENARDVDENLVDAQQTRRVLDRIVGYQISPLLWD
                     KVRRGLSAGRVQTVAVRLIVERERQIQEFKPVEYWTIDAILEVQGATKESQLITARFI
                     GIDGGRAKVEGVSAPAVGSEQEARAIIDATTKAAWSVASVDKKERRSNPYPPFITSTL
                     QQQAAARLGFSVKRTMGVAQRLYEGVDLGPDGSIGLITYMRTDSTRVSPEAITAVREW
                     IGRKVGSQYLPTQPNAYRSKKDAQDAHEAIRPTDPGLHPNKIRRYLSDEQYKLYKLVW
                     ERFVASQMVPAIYDVTTVEIDAKNQKTYNFRVSGSVLKFDGYLKIYDPEEDDEEDLPE
                     IPMGAKLTLATPEHLSGIAAQERATKLAHARRRYDQDIARIQARNEETGAGEKTEEIP
                     AFVEPEIEVASRPSAVHAQGKATEPPPRYNEASLVKELEERGIGRPSTYASIITTIQD
                     REYVTKVPPTGRGGRFVPTEIGTVVTNLLVKNFPYIFDTTYTAKLEEELDEIEDGTEK
                     WTDLLKGFYGHFEEELKKAEKHMENVKRMEKATDEKCDLCGSPLILKWGKFGSFYACS
                     AYAKEKPVPVTATGWKKEPKKVLSRVEAKLKYPMLVKTTENDESTTVEVKDKAELKSA
                     LDSVVGTGRKVSVEQVSCGFTKEHYEAKPDLQNGEQAAPAEEYCENCGKVMVLRRGPF
                     GAYMACPDYNADPPCKTVRRLSQKQQQKPAVPLDEKCPKCGKQLVLRTGTYGEFVSCS
                     GYPKCKYIKQNLIEGLKCPKCGEGDIAERKARTGNVFWGCTRYPKCDFTSNLKPVAQS
                     CPQCGSPYVVERVMDGGLYLVCPNNKEMMPRRRPRKGQKAEEALNAVECHFSERVGDA
                     PAPEKAAMPTAATHGPVVEEEQPVG"
     gene            7276..7896
                     /locus_tag="VHE33_11810"
     CDS             7276..7896
                     /locus_tag="VHE33_11810"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT98183.1"
                     /translation="MDRKSHWETIHREKPADAVSWYRPHLDVSLRLIEEVAPDRSAAI
                     LDVGAGQSTLVDDLLERGYERITVLDIAQAAIDGSRKRVGARGHVVRWQVGDVRTIEL
                     QPAACNVWHDRAVFHFLTAAADRAAYVRQAGRALKPGGHLILGAFAVMGPGKCSGLDV
                     VRYDAATLEKEFGSGFRLVKTVEEEHRTPSGVTQPFLYGCFRIESP"
     gene            8076..8900
                     /locus_tag="VHE33_11815"
     CDS             8076..8900
                     /locus_tag="VHE33_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98184.1"
                     /translation="MSAVIAAVPGWAQGPPYQTDDPVPVDLHHYEFYIFGVTDGTPVE
                     MDAAGPAFEFNWGAIPRVQLHAVLPFGVIAPLHHPAYAPDQGQGPTEYGLIDMELGAK
                     VAWIKESKYVPQIGTFTMFEMPTGNYDKGLGIGKVWYKLPVWMQKNIGPWLLDGGAGE
                     TVIPQEDFHDFPYGGFLVKYKFGERLELGGEIFSHGAEGMAPPQYQASTMVDLGGYYH
                     FKRNPNEQFLFCYGHSVAGQTENYAYVGMYWTWGRDDQSGASPGFLRDALAQPGMP"
     gene            complement(8979..>9926)
                     /locus_tag="VHE33_11820"
     CDS             complement(8979..>9926)
                     /locus_tag="VHE33_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98185.1"
                     /translation="NGTEILPGTIDPQYTAGFVWNRQYGFRVAKGWGNKTWLGFSLEN
                     DQALPGGSGGGNQFLGSPGVGGGLYNSTANYSFNRAPEVVAKMAFEPGWGHWEVFGVG
                     RFFRNRIYPNATATPASSAGAYNDTATGGGIGGGFRVPMAKKTVSLGLKGLWGQGIGR
                     MGDSTIADIAFRPDGTISPIHGFSALSTLEANPTKRLSIYLNYGGDYVSRDIHGTTGY
                     GLYSANMSGCNTEAVGTGSYAPAGTASCSGNNKDVQEATAGWWYNFYNGPKGRFRYGI
                     QYSYFTRNLWSGNGGTTNPGGNAKGTDNLVETSMRYYLP"
BASE COUNT         2004 a         2462 c         3509 g         1951 t
ORIGIN      
        1 cggaggtgcg agaggaggag tcgagggcga cgcgggcgac tcccgacatg tcttcggggg
       61 cgattccgtc gggatggcgg acgacgagga ggatggagcg gacgcggtct ttggacgcga
      121 tggtgcagcc ggggatgacg gcgagcgacg gggtgaaggc gagggcggag acggggacga
      181 ggccgatgtc ggcgtcgccg gaggcgagtt tggtggcgca ttcagcgggg gtgtcggagg
      241 tgagggcgta gcgctgggcg aggcgggagc ggtcgggctc gtgctcgaag ctccacatta
      301 agggcgcggg attgaggaag tggatggcgg agacgcggag gcgagtgtgg ggcactgggt
      361 agtttagcaa tgggctttgg tgatcacgag gacgacggtt ctatttgatc gcaaaggcgc
      421 atcaggtggt tttgccgccc aggcagtgcc tcagcagctt ctctattgtg ggcgatcacc
      481 gaagcaccga gcctgattgc ctatactgtc acggcggggg gctgggaatt gttacacctg
      541 ctacagcggc accgattgcc catcaaagcc catttggcgt cttccctcgt tctggcctat
      601 gcccttcccg aaaatgtgct gcaacccttt ttgacgccag ggctggagct cgacacttat
      661 cgcggcttcg gatttctggc gatcgccatg gtgcagacac gcgagctgcg tcctgcgttt
      721 ctgccccctg gcttcgggtt gaatttcttt ctgaccgggt accgcatctt cacacgatat
      781 cggacattgg ggggacggtc gttgcgcgga ttgcgcatcc tgcggagcga tactgatcgc
      841 agatcgatgc ggacgctggg gaatttgctt acgcattacg gttatgagct gtctcgttgt
      901 cgcgtcctgc aaacggagaa cagttaccaa gtagaaatct gcacgccaaa gggcgatgcg
      961 gacctgaagg tggtggcgga tcttgcgacc gagcctcaat ctccgccacc cgggtcgcct
     1021 tttgctgaca tgcgcgaagc gcgcaaattc gcgggaccgc tgccctttac attcgactat
     1081 gaacgacaga ctcattccgt cattcgagtc gaaggggttc gccaggcgtg gaacccacgc
     1141 ccagtttccg tggaggtgca acgaaacacc tttctcgaaa gggagccgtt tcgttccgcg
     1201 aagccgactc tggccaacgc tttctatctt gagaatgttc cctattcctg gaagccgggc
     1261 attcgggaaa ccctgccatg atgagcaagg gcgcgagcgg acgattcgaa ggggtcatca
     1321 agatcgtccg attcaatgtg cgattctacg tgctcagtgg gatgggcatc atcggcgttg
     1381 cggttttgct cgcttcgcgg aagctgccgc cgtggttggg tgtgattctt ctttgcgggg
     1441 cagctgtcgg ggctttctgg actctgagtt cacttcttgt ctcgtggtat gtctacgacc
     1501 atgccggcgt tacacgttgg ctatggctgc ctgagcgaat tcctgctcct ccgaaggtat
     1561 gggcgaatat ccatgccggt ttggacgagt ccacggctgt cctgcggaat ctgtttccgg
     1621 ggacggaagg gttagtcgta gacatctatg acccggcaga aatgaccgag ccttccattg
     1681 cacgagcgcg agagctgcat cctgcaacgg aggacttccg gcgcggcaga tacgatgctt
     1741 tgcccttgtc cgaaagtagc tgcggtgctg tcttcgtgct gttcgcagct catgaggttc
     1801 gcgcccctga acatcgctcg cggctgctgc gagagacccg ccgcgtgttg ggagagggcg
     1861 gatatgtggt gctggtggaa catcttcggg actggaaaaa ctttatcgcg tttggacccg
     1921 gattcctcca ctttcactcc cgccgaagct ggctcaacgg gattcgagag gcgggcctaa
     1981 cgattgacca tgccggacct gtcacgcctt ttgtcgagtg cttcgtgctt cgcaagggga
     2041 ggccatgaag ctgaattttg ccgaacaact gctggtcaac aatccttttc gcacagctgt
     2101 gcagaggtat tacgaaggtc ctctgttgcg cagattaggg ggagtggttt cggttggcac
     2161 agtattggaa gtgggctgcg ggcggggtgc gggagttgag gtgatcctgc ggcaatttcg
     2221 tgcaacacgt gtatttggca ttgaccttga tcctctccag atcgaacgtg ctcgaaagcg
     2281 gcttcaaggc aaatactctg atcgcgtcac cgttatgcag ggagatgcgg agcaattgtc
     2341 ctttgcgaat gaaaccttcg acgcggtgtt cgatttcggt gcgctccatc acgtaccgga
     2401 ctggcagagg agtatttcag aaatccggcg cgttcttcga cccggcggaa cgttcttttt
     2461 tgaagaagtg acgagagctg ctctcgaacg ctggatctat cgaagattcc tcgatcatcc
     2521 aggggaaaac cgcttcagtg aggccgaatt cctggaagaa ctggggagac aagggatgga
     2581 accgggcact cgcctgaggc ggacactgtt tggcgacatc tttatcgggg ttgcaaaggt
     2641 ttcctgatct gcagatccgc tttatccctg cgggcacgat ttcccgcgtt cggttgtaat
     2701 tggccagaat cgagcgtgtt cctgtcgcca tcggtaatct tgaccccctg gaaacgagga
     2761 acgtaagctc ccggcaggtg tccatggaaa cgcaaagaaa tctcacgcgg ccgatgggtt
     2821 cgcgcggggt tgcgcgatga caacggcaac tcccccatct gtaacaagca cttcttttga
     2881 agagaagcat gcgtggctgg ggttggcgct gacgcctggg attgggccac ggcgtttgct
     2941 gcgtgcggtg gagcggtacg ggagtgcggc tcgggtactg catttgccgc tgacggaact
     3001 ggaggcgttg cagcttccgg ccgagtcggc gcagtttatc tcggggggtg aggcagcgca
     3061 tgcggcggat caggagctgg agaagctggc ggcctcgggt gcgtcgattc tgacgtggca
     3121 tgacgaagcg tatccgctgc ggctgcggga gatctacgat ccgccggcgc tcttgtgggt
     3181 gcgcggcgat gcgagtttgc tggcgagacc gagcctggcg gtggtgggaa cccggcatcc
     3241 gacgccgtat gggacgggga tagcggcgaa gctggcgcgg gagctggcga tgcgggggct
     3301 gatcattttg agcgggatgg cgcgcggggt ggatacagcg gcgcacaagg gggcgctcga
     3361 ggccaaacgg ccgacgattg cggtttgggg aaccggggtg gacgtgattt atccgaagga
     3421 gaacaagtcg ctggcggagc agattgttca agggggcggg ctgatccttt cggaattgcc
     3481 gatggggacg tttccggcgc cgcagaattt tccgaagagg aaccggatcc tgagcgggat
     3541 gagcgtggga gtgctggtga ttgaagcggc ggagaactcg gggacgcggg tgacggcgcg
     3601 gtgcgcgctg gagcagaacc gggaggtgtt tgcggttccg gggaacgtga cgacgaaaaa
     3661 cgcctggggt cctaacctgt tgataaagca aggcgcaaag ctgacatcct gctgggagga
     3721 cgtgtgggag gagttgccta cacaaataag gatggagctg gagcaaagcg atggatttgc
     3781 atccacggca ggatctgagg catcgttatt cgaggaatca ccgctgccgc cgtcggaggc
     3841 gcgcgtgctc ggcgtgcttc gaatggatga agcactgcag atggatgaaa tcctcgaaaa
     3901 gctggagccg gacgtgagct cctcagaggt tttcacagcg ctgttcgagc tggaactggc
     3961 gggccgcata cggcaacttc ctggaaagaa ctacgtcagg agtttgtgag ttttggctgc
     4021 ccggttgcac ggctgggtac gcaatctgcc ccggatgtgt agattcaaag cggtagtcca
     4081 tggtagcttc cggaaggaag aagccagaaa gaactgtgca gcagaccggt gcgacgtgca
     4141 cggggttgcc gcagtctaag ggaaacgatg agtaaatcgc tggtcatcgt cgagtcgccc
     4201 gcgaaggcga agacgatcaa taagtatctg ggcaaagact acgaggtgga agcctccttc
     4261 ggccacatca tggatctgcc gaagcgggac attggtgtgg agctggagcg gagaacgttc
     4321 gagccgacgc tgatcgtgtc tccggacaaa gagaaggtgg ttgcgaagct gcggaagatg
     4381 gcgtcgaagg cggacgcggt atatctggcg cctgacccgg atcgcgaagg ggaggcgatc
     4441 gcggcgcatt tgtcgctgca gctgatgccg gtggtgaagg acaaacggcg catccgtcgc
     4501 gtgacgttca acgagatcac gcagaaggcg gtacgcgcgg cgttcgaaaa tgcacgcgat
     4561 gtggacgaga acctggtgga cgcgcagcag acgcggcggg tactggaccg gatcgtgggc
     4621 tatcaaattt cgccgctgct gtgggacaag gtgcggcgcg gactctcggc gggtcgcgtg
     4681 cagaccgtcg cggtacggtt gattgtcgaa cgggagcggc agattcagga attcaaaccg
     4741 gtcgagtact ggaccatcga cgcaattctt gaagtgcagg gtgcgacgaa ggaatcgcag
     4801 ctgatcacgg cgcggttcat cgggatcgat ggagggcgcg cgaaggttga gggtgtgagt
     4861 gcacccgcgg tgggcagcga gcaggaagcg cgcgcgatca tcgatgcgac gacaaaagcc
     4921 gcgtggagcg tggcgtcggt cgataagaag gagcggagga gcaatccgta tccgccgttt
     4981 attaccagca cgctgcagca gcaggcagcg gcgcggctgg gattcagcgt gaagcgcacg
     5041 atgggtgtgg cgcagcggct ctatgagggc gtggatctcg ggcccgatgg gtcgattgga
     5101 ctcatcactt atatgcgtac cgattccacg cgggtttcgc cggaagcgat cacggcagtt
     5161 cgggagtgga ttggcaggaa agtcgggagc cagtatttgc cgacgcagcc gaacgcgtat
     5221 cgctcgaaga aggatgcgca ggatgcgcac gaggcgattc ggccaaccga tccggggctg
     5281 catccgaaca agattcggcg ctacctgagc gacgagcaat acaagctcta caagctggta
     5341 tgggagcgtt tcgtggcttc gcagatggtg ccggcgatct acgacgtgac gacggtggag
     5401 atcgatgcga agaatcagaa gacgtataac ttccgcgttt cgggatcggt gctgaagttc
     5461 gatggatatc tgaagattta cgatccggaa gaagacgacg aagaggatct gccggaaatt
     5521 ccgatgggcg caaagctgac gctggcgact ccggagcatc tgtcgggcat tgcggcgcag
     5581 gagcgtgcga cgaagctggc acatgcgcgg cggcggtacg accaggacat tgccaggatt
     5641 caggcgcgca atgaggagac gggcgccggc gagaagaccg aggagattcc tgcgtttgtc
     5701 gagccggaga tcgaggtagc atcgcggcct tcggcggtgc atgcacaggg caaggccacc
     5761 gagccgccgc cacgctacaa cgaagcttcg ctggtgaagg agctggaaga gcggggcatt
     5821 gggcggccgt cgacgtatgc atcgatcatc acgacgattc aggaccgcga gtacgtgacc
     5881 aaggttccgc cgacggggcg gggcggacgg ttcgttccca cagagatcgg caccgtggtg
     5941 acgaatctgc tggtgaagaa cttcccgtac atcttcgata cgacgtacac ggcgaagctg
     6001 gaagaggagc tggacgagat tgaagatggc acggagaagt ggaccgatct gctgaagggc
     6061 ttctacgggc actttgagga agaactgaaa aaggccgaga agcacatgga gaacgtgaag
     6121 cggatggaga aggccacaga tgagaagtgc gatctgtgcg gctcgccgct gattctgaag
     6181 tggggcaagt tcggcagctt ctacgcgtgc agtgcgtacg cgaaggaaaa gcccgtgccg
     6241 gtgacggcga caggctggaa gaaggagccg aagaaggttc tgtcgcgcgt cgaagcgaaa
     6301 ctgaagtatc cgatgctggt gaagacgacc gagaatgacg agtcgacaac cgttgaagtg
     6361 aaggacaaag ctgagctgaa gtcggcgctg gacagtgttg ttggcaccgg ccgcaaggtc
     6421 agcgtagagc aggtgagctg cggattcacg aaggagcact acgaggccaa gccggatctg
     6481 cagaatgggg agcaggctgc gcctgctgaa gagtactgcg agaactgcgg caaggtgatg
     6541 gtgctgcggc gcggaccgtt tggggcatac atggcgtgcc cggactacaa cgccgatccg
     6601 ccgtgcaaga cggtgcggcg tctcagccag aagcaacagc aaaagccagc ggtgccgctg
     6661 gacgagaagt gtccgaagtg cggcaagcag ctggtgctgc gcacgggaac gtatggggaa
     6721 ttcgtctcgt gctccggcta tccgaagtgc aagtacatta agcagaatct gattgaagga
     6781 ctgaagtgcc cgaagtgcgg cgagggagac attgccgagc gcaaagcgcg gaccgggaat
     6841 gtgttctggg gctgcactcg ttatccgaag tgcgacttca cgtcgaatct gaagccggtg
     6901 gcgcagagct gcccgcagtg cggatcgccg tacgtggtgg aacgggtgat ggatggcggc
     6961 ctgtatctgg tatgccccaa taacaaggag atgatgccgc ggcggcgtcc gcggaagggg
     7021 cagaaagcgg aggaggcgct gaacgcagtc gagtgccact tcagcgagcg tgtcggggat
     7081 gctccggctc cggagaaggc ggccatgccg acggcggcaa cacatggacc ggtggtggag
     7141 gaagagcagc cggttgggtg agagcagcta agggcctgtt agcaaggagc ggttagctaa
     7201 gaagcggtca gctgaaaagc ggttagctaa aagcgttcag cttgatgggg ccgttgcggg
     7261 atccgctggc acaggatgga ccgaaagagt cactgggaaa ccattcaccg ggagaagccg
     7321 gcggatgcgg tgagttggta tcggccgcat ctggatgtgt cgctgcggtt gatcgaggag
     7381 gtagctccgg atcggtcggc tgcgattctc gatgttggcg ctggacagtc gacgctggtc
     7441 gatgaccttc ttgaacgagg ctacgagcgg atcaccgtcc tggacattgc gcaggctgcg
     7501 attgacggga gccgaaaacg ggtgggcgcg cgagggcacg ttgttcgctg gcaggtgggc
     7561 gatgttcgta cgattgaact gcagccagcg gcctgcaacg tctggcatga tcgcgcggtg
     7621 tttcattttc tgacggctgc agcggatcgc gcggcttatg tgcggcaggc gggccgagcc
     7681 ctgaagccgg gagggcatct gatcctcggt gcgtttgcgg tcatgggacc ggggaagtgc
     7741 agcgggctgg atgtggtgcg ctatgatgct gccaccctgg agaaggagtt tggatcaggg
     7801 ttccggttgg tgaagacggt agaagaggag catcggacgc cgtcaggggt gacgcagcca
     7861 tttttgtatg gctgttttcg gatcgaatcg ccttagccga tgcatcgaac ggagaattcg
     7921 ggcgcagaga atttgagagc gattcaccgt tcgctttgtg gccgggccgg atcatggtgg
     7981 gtgcgttcga gatggaggca atcacagtga tgttccacgc cgacgctttg cgtctactca
     8041 gcagacgaca taaaagagcg atacctctgc tgcttttgag tgcggtgatc gcggccgtgc
     8101 cgggatgggc gcagggaccg ccgtaccaga cggacgatcc ggtgccggtg gaccttcatc
     8161 actacgagtt ctatatcttc ggcgtcaccg acggaacgcc ggtggagatg gatgcggcag
     8221 gaccggcgtt tgagtttaac tggggagcga ttccgcgagt gcaactgcat gcggttttgc
     8281 cgtttggcgt gatcgcgccg ctgcaccatc ctgcctacgc accggaccag ggacaggggc
     8341 cgacagagta tgggctcatc gatatggaac tgggggcgaa ggtcgcctgg atcaaggaaa
     8401 gcaaatatgt gccgcagatc ggcacgttca cgatgttcga gatgccgacg gggaattacg
     8461 acaagggact tggcattggg aaggtctggt acaagctgcc ggtgtggatg cagaagaaca
     8521 tcggcccatg gcttttggat ggcggcgcgg gggagacggt gattccgcag gaggattttc
     8581 acgactttcc ctacggcggg ttcctggtga agtacaagtt tggcgagcgg cttgaactgg
     8641 gtggggagat tttctcgcat ggggcagagg gcatggctcc cccgcagtat caggcttcga
     8701 cgatggtcga cctgggcggg tactaccact tcaaacgcaa tcccaacgaa cagttcttat
     8761 tttgctatgg gcattcggta gcgggacaga cggagaacta cgcgtacgtg ggaatgtact
     8821 ggacgtgggg cagggacgat cagagcggcg cctctccagg gttcctgcgg gatgcgttgg
     8881 cacagcctgg aatgccgtag aaatctgcca gattcatccg cgatgaaaag aaaggacccg
     8941 ctggagcggg tccttttcga tttgagaatg aattgagact agggcaggta gtagcgcatg
     9001 gaggtctcga cgagattgtc ggtacccttc gcgttgccac cgggattggt ggtccctccg
     9061 ttgccggacc agaggttgcg ggtgaagtag ctgtactgga tgccgtagcg gaagcggcct
     9121 ttgggaccgt tgtagaagtt gtaccaccag ccggcagtgg cttcctgaac gtccttgttg
     9181 ttgccggagc aggaggcggt tccggcagga gcataagagc cggtgcctac cgcttctgta
     9241 ttgcagccgg acatgttggc ggaatagagg ccgtagccag tggtgccgtg aatgtcgcgg
     9301 ctcacgtagt caccgccgta gttgaggtag atgctcaggc gcttggtggg attcgcctcc
     9361 agcgtgctga gtgcggagaa gccgtgaatc ggggagatcg tcccatcggg acggaaggcg
     9421 atgtccgcta tggtcgagtc acccatgcgg ccgatgccct gtccccagag ccccttcagg
     9481 ccgaggctga cggtcttctt cgccatggga acgcggaagc cgccaccgat gccgccgccg
     9541 gttgccgtgt cgttataagc tccggcagaa ctcgcgggcg tggctgttgc gttggggtag
     9601 atgcgattgc ggaagaagcg gccgacgccg aagacctccc agtgtcccca gccgggctcg
     9661 aaggccatct tggccacgac ttccggagcg cggttgaagg agtagtttgc ggtcgagtta
     9721 tacaggccgc cgccgacgcc gggtgagccg aggaactgat tgccgccgcc gctgccgcca
     9781 gggagagcct gatcgttttc aagcgagaag ccgagccagg ttttgtttcc ccagcctttg
     9841 gcaacgcgaa agccgtactg gcggttccac acgaagccgg cggtgtactg cggatcgatg
     9901 gtgccgggca ggatttcggt tccgtt
//
LOCUS       DASZYN010000457         2349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1115639, whole genome shotgun sequence.
ACCESSION   DASZYN010000457 DASZYN010000000
VERSION     DASZYN010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115639"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2349)
                     /locus_tag="VHE33_11825"
     CDS             complement(<1..>2349)
                     /locus_tag="VHE33_11825"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HVT98186.1"
                     /translation="TDTNGNWELAPWALDACIQVAAAIGEPEALYLPMSMERIEVFRS
                     MPETVRSWVRIARLDADNISATILVTALDGSVILQISQLRFRRKSERPQTVPLYRLEW
                     EPAELSADRMAKLANELNASIPDRAAQKDAGDYVALFEQMEALSAAYVCEAFLKLGWP
                     EAERRLSHQELERRWHVSPKHRPLLRRMLEIAKEAGAVVQDGDLCRFLATPRPVAEAQ
                     VQSLKQRFPQGSTEISLLRRCGAALADVLTGEIEGRELVFPGGSSDEMEQLYRGSLPA
                     KIYNNMVAEAVVRLAKAKNGAVRILEVGGGTGATTEVVLGSLREVQVVPQEYLFTDIS
                     PLLVRRTQERIGTEPFFRAQTFNLEQDAAAQAISGRFDVILAVNVLHATSDIRATLRC
                     LRNLLTGDGHLLITEVIGKQRWSDLTVGLLEEWWRFTDRDLRPDHPALPAGRWKQVLL
                     DEGFADVRAVPATGHGLFAYQELLLAGGVSAERSVIVIGEDELAEKIAVKLRARLVTA
                     ADMSSLQQIVADAVVWVAPHQPEAAAGITESATDQVRSLLSTSTAMTNFASALRLYVV
                     TRSAIPVPGHSGSLIDTISAGLATGIASERPELRCTRIEVTGEDPEYDAALIASEVNT
                     DNDEPWISLRKGGRSTTRLAPLEVSPLANVELTGRSGIDTLHYQPATRRELAPDEVRI
                     AVYSTALNFRDVLQASGVVDLQAPMGTDCAGVIVEVGAEVAEFRAGDEVVAIAAGCFS
                     SEVVASSALTVRLPAGLGVAQASAQAVAWLTAEYALCEVGGLK"
BASE COUNT          467 a          697 c          718 g          467 t
ORIGIN      
        1 tttcaaaccg ccgacttcgc agagtgcgta ctcggcggtc agccaggcga cggcttgtgc
       61 agaggcctgg gcaacgccga gacctgccgg cagtcgaacc gtcaacgcac tggaggcgac
      121 cacttcgctt gaaaagcaac ccgctgcgat ggcgacaact tcatcgccag cgcggaattc
      181 cgcgacctct gcaccgactt caacgatgac tcccgcgcag tcggtaccca tgggcgcctg
      241 gagatcgacg acgccggagg cctgcagaac atcgcggaaa ttgagagccg tggaataaac
      301 ggcaatgcgg acctcgtccg gggctaattc ccgtcgtgtt gccggctgat agtggagcgt
      361 gtcgattccg cttctaccgg tcagctcgac gttggccaga ggcgacactt cgaggggcgc
      421 aaggcgggtt gtcgatcgac ctcccttgcg caacgagatc cagggctcgt cgttgtcggt
      481 gttcacttcc gaagcgatca aggcagcatc gtactccgga tcctctcctg tgacctcgat
      541 gcgcgtgcaa cgcagctcgg gacgttcgga ggcgattccc gttgcgaggc ctgccgagat
      601 agtgtcgatc aggcttcccg aatggccagg cactgggatg gcgctgcggg tgacgacata
      661 gagtcgcagg gcagaggcga aattcgtcat cgccgtggaa gtgctgagaa gagatcgaac
      721 ctgatctgta gcggattctg tgatgccggc tgcggcttcg ggttgatggg gcgcgaccca
      781 cacgacggcg tcagcgacaa tctgctgtaa cgaggacatg tccgctgccg tcaccagccg
      841 cgctcgcaat ttcactgcaa ttttttcggc gagctcatcc tctccgatca caataacgga
      901 gcgctctgca gagacgcctc cggcgagaag gagttcctga taggcaaaga gcccgtgccc
      961 ggttgccggc acggcacgca cgtcggcaaa tccttcgtcg aggagcactt gcttccaacg
     1021 tcccgccggc agtgcgggat gatcggggcg gagatcgcga tcggtgaagc gccaccactc
     1081 ttcgagcaaa ccgacggtga gatcggacca gcgttgtttt ccgatgacct ctgtgatcag
     1141 cagatgacca tctccggtta gtaggttcct gaggcaacgc agggtcgcgc gaatgtccga
     1201 tgtggcatga aggacattta cggcgagaat gacgtcgaag cggccggaga tcgcctgtgc
     1261 cgcagcatcc tgttccaggt tgaaggtctg tgcgcggaag aatggctcag ttccgatgcg
     1321 ttcctgggtc cggcggacca gaagaggaga gatatcggtg aacaggtatt cctgaggaac
     1381 gacctgaact tcgcgaagag agccaagaac cacctctgtg gtcgcaccag ttcctcctcc
     1441 aacttcgaga atgcgaacgg cgccattttt cgctttggca agccgaacta ctgcttccgc
     1501 caccatgttg ttgtagatct tcgccggcag agagccgcga taaagctgct ccatctcgtc
     1561 gctcgaaccg ccgggaaaga ccagttccct gccttcgatc tcgccggtga ggacatcggc
     1621 gagcgccgct ccgcagcgtc tcagaagaga aatctctgtc gatccctgtg ggaaccgttg
     1681 tttgagactt tgaacctgcg cttctgccac cggccgtggc gtggcaagga atctgcagag
     1741 gtccccgtcc tggacgacag ctccggcttc tttggcaatc tcgagcatgc gccgcaggag
     1801 aggccgatgt ttgggtgaga catgccagcg tcgctcgagt tcctgatggc tgagccgtct
     1861 ttcggcctcc ggccagccca gtttcaggaa cgcctcacag acataagcgg ccgagagcgc
     1921 ctccatctgc tcaaagagtg caacatagtc gcccgcatcc ttttgcgccg cgcgatccgg
     1981 gatggatgcg ttcagttcat tggccagctt tgccatccga tcagcggaca actctgccgg
     2041 ttcccattcg agccggtaga gtggaaccgt ctgcggccgc tcgctcttcc gccggaaccg
     2101 gagttgcgag atttgcagaa taacggaacc atcgagggcg gtgacgagga tggttgcgga
     2161 aatgttatcg gcgtcgagcc gtgcgattcg tacccagctg cggaccgtct ccggcatgga
     2221 ccggaagact tcgattcgtt ccatgctcat cggcaaatag agagcttccg gctcaccgat
     2281 tgccgcagcg acctggatgc acgcgtccaa agcccatgga gcgagctccc aatttccatt
     2341 tgtatctgt
//
LOCUS       DASZYN010000458         2794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_213294, whole genome shotgun sequence.
ACCESSION   DASZYN010000458 DASZYN010000000
VERSION     DASZYN010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213294"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2746)
                     /gene="alaS"
                     /locus_tag="VHE33_11830"
     CDS             complement(<1..2746)
                     /gene="alaS"
                     /locus_tag="VHE33_11830"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HVT98187.1"
                     /translation="MPSSKPDSTNTGKWSGSAIREMFLKFFETKGHRRVHSSSLVPAN
                     DPTLLFTNAGMNQFKDVFLGLERRDYARATTSQKCVRAGGKHNDLENVGFTRRHHTFF
                     EMLGNFSFGDYFKKDAIAYAWELVTSPQWYGLPKDELYVTIFEGDAEVPRDDEAERLW
                     MDVGVPKERIFTYGRKDNFWQMGETGPCGPCSEIFYDMGMEAAETPGVDRPFGEDEAR
                     YVEIWNLVFMQFDRSAVKDAKGAVTEYTLTPLPKPSIDTGMGLERTAAVLQGVVSNFE
                     TDLFVPLIERAAELTGFVSGHDFSRAEKPAQTPGASAPASAHSLTGNASLRIIADHAR
                     ASTFLISDGVLPSNEGRGYVLRKILRRGIRHGKLLGQEKPFMSQMVYAVRDEMQDAYP
                     ELKESADRVAKVVEAEEKQFARVLEIGTARLDQAIAEGKLDGRAAFHLYETFGLPLDF
                     MMDAARDAGVHFDLEGFEAARAEEQARARASWKGGAQKTASPVFRDLPKTAFEGYRQL
                     RTDGAEVLAIVKDGQGVPAAGVGDAVEIVLDRTSFYADSGGQIGDTGWLYSDDHNSVV
                     AEVNGCTKPVQGVFAHRAILRQPVKLGDKLDTVVDANFRNATRRNHTATHLLHAALRE
                     VLGTHVKQAGSLVEPNRLRFDFSHFAGIADEELQDIEDIINRQTLGNTKVETLEDVPI
                     DVAVNEYHAMALFGEKYGEKVRVVKIGDFSTELCGGTHTGATGEIGVVKLLGEGSVSS
                     GVRRVEAVTGLGALSEFRKGFEVAQIASQLGPSDGSPADALRQRLSAQEEELKKLRRE
                     LDEARMKSAASRVSGAADQAVDVAGFKVLAQRVDGLDRGQMRTLVDNLRNKLGSGVVV
                     LGGAQEDGKVALIVGVTKDLTSKVQAGKVVGEIAKKVGGSGGGRPDMAEAGGKDASAL
                     D"
BASE COUNT          494 a          990 c          773 g          537 t
ORIGIN      
        1 catccagcgc cgacgcatcc ttcccgccgg cctccgccat gtccggacgg ccgccgcccg
       61 atccgcccac cttcttcgcg atctcgccga cgacttttcc cgcctgcact ttgctggtca
      121 ggtctttcgt gacgccaacg atgagggcca ctttgccatc ctcctgtgcg ccgcccagca
      181 caacgacgcc cgaccccagc ttgttgcgca aattgtcgac gagcgtccgc atctgcccgc
      241 ggtccagccc atcgacgcgc tgcgcgagca ccttgaatcc cgcaacatcg acggcctgat
      301 ccgccgcgcc cgacacgcgc gacgccgccg acttcatccg cgcctcatcc agctcccgcc
      361 gcagcttctt cagttcctct tcctgcgcgg acagccgctg gcggagcgca tccgcgggcg
      421 atccatcgga tggccccagc tgcgaggcaa tctgcgcgac ctcgaagccc ttgcgaaact
      481 cgctcagcgc gccgagtccg gtgacggctt cgacgcgccg cacgcccgac gagaccgagc
      541 cctcgcccag cagcttcact acgccgatct cgccagtcgc tccggtgtgc gtgcctccgc
      601 acagctccgt cgagaagtcg ccgatcttca ccacgcgcac cttctcgcca tacttctcgc
      661 cgaacagcgc catggcgtga tactcgttta cggccacgtc gatgggaaca tcttcgagcg
      721 tctccacctt cgtgttcccc agcgtctgcc gattaatgat gtcctcgata tcctgcagct
      781 cctcatcggc gatcccggcg aagtgggaaa aatcgaagcg cagccgattc ggctcgacga
      841 gagaacctgc ctgcttgacg tgggtcccca gcacctcgcg cagcgccgca tgcagcagat
      901 gggtcgcggt atgattccgc cgcgtcgcat tgcggaagtt tgcatcgacg acggtgtcga
      961 gcttgtcacc caacttcact ggctgccgca gaatcgccct gtgggcaaat accccctgca
     1021 ccggcttcgt gcatccattc acttcggcga cgaccgaatt atggtcatcg gaatagagcc
     1081 atcccgtgtc gccgatctgg ccgcctgagt cggcgtagaa gctcgtgcga tccagcacga
     1141 tctcgaccgc gtcgcctacc ccggctgcgg gaacgccctg cccgtccttc acaatggcca
     1201 ggacctcggc cccatccgtc cgcagttgcc gatagccttc gaaggcggtc tttggcagat
     1261 cgcgaaagac aggactcgcg gtcttttgcg cgccgccctt ccaggaagca cgggcgcgcg
     1321 cctgctcctc ggcgcgcgcc gcctcgaagc cttcgaggtc gaaatgaacg cccgcatcgc
     1381 gcgccgcgtc catcatgaaa tcgagcggca gaccgaatgt ctcgtacaga tggaacgcgg
     1441 cccgcccatc gagcttgcct tccgcaatcg cctggtccag ccgcgcggtt ccaatctcca
     1501 gcacgcgagc gaactgcttc tcctccgcct cgactacctt ggccacgcgg tcggcgctct
     1561 ctttcagctc cggatacgca tcctgcatct cgtcccgtac ggcatagacc atttgggaca
     1621 tgaagggctt ttcctggccg aggagttttc cgtgacggat tccgcgacga agaatctttc
     1681 gcagcacata gccgcgccct tcgttggagg gaaggacgcc gtcggagatg aggaatgttg
     1741 atgcgcgcgc atgatcggcg atgatgcgga ggctcgcgtt gccggtgagg ctgtgcgcgg
     1801 acgcaggggc tgaagccccg ggtgtttggg ccggcttttc ggcacgactg aagtcgtgcc
     1861 ctgatacaaa accggtcagc tccgcggctc gctcaatcag gggcacgaaa agatccgtct
     1921 cgaagttgct gacgacgccc tgaagcaccg cggcggtgcg ctccaaaccc atgcctgtat
     1981 cgatggaggg cttgggcaaa ggggtcaacg tgtattcggt caccgctccc ttcgcatcct
     2041 tcaccgcaga gcggtcgaac tgcatgaaga ctaaattcca gatctcgacg taccgcgcct
     2101 cgtcttcccc aaacggccgg tccacgcctg gagtctctgc ggcctccatg cccatgtcgt
     2161 aaaaaatctc cgagcatgga ccgcacggcc ccgtttcccc catctgccag aagttgtcct
     2221 tgcgcccgta ggtgaagatg cgctcctttg gaacgccgac gtccatccac agccgctctg
     2281 cctcgtcatc gcgcggaacc tcggcgtctc cttcgaaaat cgtcacgtac agctcgtcct
     2341 tcggcagccc gtaccactgc ggcgacgtca ccagctccca tgcatacgca atggcgtcct
     2401 tcttgaagta gtctccgaag ctgaagttcc ccagcatctc gaagaaggtg tggtgccgtc
     2461 gcgtgaaccc gacattctcc agatcgttgt gcttcccgcc cgcccgcaca cacttctgcg
     2521 atgtcgtggc gcgcgcgtag tcgcgccgct ccaggcccag aaacacatcc ttaaattggt
     2581 tcatccccgc gttggtgaag agcagcgtag gatcgttggc cggcaccagc gacgacgagt
     2641 gcacgcgccg gtgacccttc gtctcaaaga acttcagaaa catctcgcgg atggccgacc
     2701 cggaccactt acccgtattg gttgaatctg gcttactgga aggcatacac cccgagttta
     2761 ccggagcagg accggcccat tcctgaacag actg
//
LOCUS       DASZYN010000459         3484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_471774, whole genome shotgun sequence.
ACCESSION   DASZYN010000459 DASZYN010000000
VERSION     DASZYN010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471774"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /gene="pssA"
                     /locus_tag="VHE33_11835"
     CDS             complement(<1..719)
                     /gene="pssA"
                     /locus_tag="VHE33_11835"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HVT98188.1"
                     /translation="MNPAGMNSDPQTFLRPTGASKFHPPAPRRRPRGMFLLPSMFTAV
                     NIAAGFFAITQSLEGSASQPHHFDYAALAIGLAIPFDALDGRIARLTNTESDFGRELD
                     SLADVITFGVAPSILAFAWGFRSLPAAMPHELRLKLVQFGAFVCFLFLLCGASRLARF
                     NISVNPTPKNPGHPGRKYFVGMPIPAAAGIVAAVIHGFIGLPIFTWWLAIVWGLLVGF
                     IGFLMVSTWRFWSGKEINLTVR"
     gene            complement(716..1384)
                     /locus_tag="VHE33_11840"
     CDS             complement(716..1384)
                     /locus_tag="VHE33_11840"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HVT98189.1"
                     /translation="MVRDGYFYGLALAAVAAILWLTTRHWEWAILPLLLAAFFLWFFR
                     DPARTIPRERGLVVSPADGKVTEVARINTPDGARLRISIFLSVFDVHVNRSPVEGILR
                     DVRYKKGEYMNAMNPECAVRNEQNLAVVDCIEGYQVSFVQIAGLLARRIVFNPKPGDY
                     LERGQRVGLIKFGSRTDILLPGDAEPLVQPGDRVRGGASVVARVTNPDSNPMLAAIDA
                     ELTV"
     gene            1654..2307
                     /locus_tag="VHE33_11845"
     CDS             1654..2307
                     /locus_tag="VHE33_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98190.1"
                     /translation="MAGLLGGLAAAQSMAGYRGLPFADSRYPGGAQTIPGRVECARYD
                     RGGEGVAYHDADAANHGSGELNPLDGNYYNEFRKDEGVDTSYTKFRDTIDNSPWDRVR
                     VEENQLYVGWTEPGEWFNVTVDVARAGTYVADLLYTSNRGGSIALDVNGKDATGALTI
                     PTTYDAGDPIAWRQWHHWNLAKNLVKVQLPAGRSVLTVHILTQGNMNLAYFDFRLEG"
     gene            complement(2436..2654)
                     /locus_tag="VHE33_11850"
     CDS             complement(2436..2654)
                     /locus_tag="VHE33_11850"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HVT98191.1"
                     /translation="MDLEANGQPDSEAIRRLEEEHRHYSEKLEQLLQKHWLSEDEQLE
                     EVRLKKLKLHIKDQLTIVRSQTRAYSAA"
     gene            complement(2770..3360)
                     /locus_tag="VHE33_11855"
     CDS             complement(2770..3360)
                     /locus_tag="VHE33_11855"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT98192.1"
                     /translation="MTFDLRPLQSTDLDEIAAIATASPEAPQWPRSAYASYIAPDPAN
                     PALLRIGLAVTEVPNQARPDTQPLRDLTSEREARPQGLPVLLGFACATLLLDGTQNLC
                     QLDSMAVHPSHRRHGIGAALLRELLAWATQNGAFHFSLEVRASNTAAIALYQRFGLRP
                     EGRRTRYYAHPEEDALLLGIPITPGSPPPVFHYEIG"
BASE COUNT          696 a          853 c         1345 g          590 t
ORIGIN      
        1 cggacggtga gattgatctc tttgccgctc cagaagcgcc aggtgctgac catgaggaag
       61 ccgatgaagc cgacgagcag accccagaca atagcgagcc accaggtgaa gatgggcagg
      121 ccgatgaagc catgaatgac ggcggcgacg atgccggcgg cggcggggat aggcatgccg
      181 acgaaatact tgcggccggg gtggccggga tttttcggag tgggattgac gctgatattg
      241 aagcgggcca ggcggctggc tccgcagagg aggaagagga agcaaacgaa ggcgccgaac
      301 tgaacgagtt tgaggcgcag ttcgtggggc atggcggcgg gcagggagcg aaagccccag
      361 gcgaaggcga ggatgctggg agcgacgccg aaggtgatga cgtcggcgag ggaatcgagt
      421 tcgcggccga agtcactttc ggtgttggtg aggcgggcga tgcggccgtc gagggcgtcg
      481 aagggaatgg cgaggccgat ggcgagcgcg gcgtagtcga agtggtgagg ctgggaggca
      541 gatccttcga ggctttgcgt gatggcgaaa aagccggcgg caatgttcac agcggtgaac
      601 atggaaggca gcaggaacat gccacgggga cggcggcggg gcgcgggggg atggaacttc
      661 gaggctccgg tggggcgtag gaaggtttgc ggatcggaat tcatgccggc cggattcata
      721 cggtgagctc cgcgtcgatt gcggcgagca tgggattgga gtcggggttg gtgacgcgag
      781 cgacgacgct ggccccgcca cggacgcggt ccccaggctg gacaaggggc tcggcgtcac
      841 cggggaggag aatgtcggtg cgagatccaa atttgatcag gccgacgcgc tgaccgcgct
      901 ccagatagtc gccgggtttg ggattgaaga cgatgcggcg cgcgagcagg ccggcgatct
      961 gaacgaagga gacctgataa ccctcaatgc agtcgacgac ggcgaggttc tgctcgttgc
     1021 gtacggcgca ttcaggattc atggcgttca tgtattcgcc ctttttgtag cgcacgtcgc
     1081 ggaggatgcc ttcgacggga gagcgattga cgtgaacatc gaacacgcta aggaagatgc
     1141 tgatacggag gcgggcgccg tccggagtgt tgatgcgggc gacctcggtg accttgccat
     1201 cggccgggga gacgaccagg ccgcgttccc tggggatggt gcgcgcggga tcgcggaaga
     1261 accagagaaa gaatgcggcg aggagcaggg gcagaatcgc ccattcccag tggcgcgtgg
     1321 tgagccagag gatggcagct accgcagcga gggccagtcc gtagaaatat ccgtcacgca
     1381 ccatgagaag tcaaaagacc gtcggacgag gccagaggca gtcagcagtt tgcgattgag
     1441 ccggtcacga aacctggaat gcaggaaccg gctgcaggca acgaacgggt cgttcgtccg
     1501 aaagtctttt ccattctaga gccagatggg tgaaacagaa aggaagcggc gcgcggaagg
     1561 cattacgaac gtggcaagga ggcggagtcg gatgtaccgt ggtctgcgga ggaaaacagg
     1621 ttgacggcac gatgcgtttt ggttggggcc tgcatggcgg gcctgctggg cgggcttgcg
     1681 gcggcgcaat cgatggccgg atatcgcggg ttaccgtttg cggacagccg gtatccaggg
     1741 ggagcgcaga cgattccggg gcgggtggag tgcgcccggt acgatcgcgg cggggaaggt
     1801 gtggcttatc acgacgccga cgcggccaat cacgggagcg gggagctgaa tccgctcgat
     1861 gggaactact acaacgagtt tcgaaaagac gagggcgtcg acacgtcgta cacgaaattc
     1921 cgcgacacga tcgacaacag cccctgggat cgggtgcggg tggaggagaa ccagctctac
     1981 gtgggctgga cggagcctgg ggaatggttc aacgtgaccg tggacgtggc ccgggcgggt
     2041 acctatgttg ccgatctgct ttacacgtcg aatcggggcg ggagcatcgc gctcgatgtc
     2101 aacggaaaag acgcgacagg cgcgcttacg attccgacga cgtatgacgc gggggatccg
     2161 atcgcctgga ggcaatggca ccactggaat cttgcgaaga acctggtgaa ggtgcagttg
     2221 ccggctgggc ggagtgtgct gacggtgcac attctgacgc aggggaacat gaacctggcg
     2281 tacttcgatt ttcggctgga gggctgaggg agcgctggcg cgcggcgaat gccttgccag
     2341 gaaacgaggc aggaaattcc cagggattgc atacacccct cgcacggcga tggcactgcg
     2401 tcgcgagggg agattctttt aggaagggcc gcaggtcagg cggcgctgta agcgcgcgtc
     2461 tgactgcgga cgattgtcag ctggtccttg atatggagtt tgagtttctt caggcgaact
     2521 tcctcgagtt gctcgtcttc cgatagccag tgcttctgga gcagttgttc gagcttctcc
     2581 gaatagtgac gatgctcttc ttccaggcga cgtattgctt cactatccgg ctgaccgttc
     2641 gcttccagat ccataggact cctccggttt cgggatcgta cccggcggag ccgcggcaat
     2701 cgtggttcgc cgggcgagtt ggctcacatc agtgagacgt accaagctag caccgtggca
     2761 gcccggcctt caaccaattt cgtagtggaa aacaggaggg gggctgccgg gggtaatggg
     2821 gatgcccaga agcagggcat cttcttcagg atgagcatag tagcgggtgc ggcggccttc
     2881 gggacggagg ccgaagcgct ggtagagagc gatcgcggcg gtgttggagg cgcggacttc
     2941 aagcgagaaa tggaaggcgc cgttttgcgt ggcccaggcg agaagttcgc gcaggagcgc
     3001 ggcgccgatg ccgtggcggc ggtggctggg gtggacggcc atcgagtcga gctgacagag
     3061 gttctgcgtg ccatcgagta ataaggttgc gcaggcgaat ccgaggagga ctggcagtcc
     3121 ctgcggccgc gcttcgcgct ccgaggtcag gtcgcgaagg ggctgggtgt ccgggcgggc
     3181 ttgattcgga acctctgtga cggcgaggcc gatgcggaga agagcaggat tcgccgggtc
     3241 gggcgcgatg taggaagcgt aggcggaccg cggccactgg ggagcttcgg gggaagcggt
     3301 tgcgatggct gcgatttcgt cgaggtcggt ggactggaga ggacgaaggt cgaaggtcat
     3361 gcgcgggagg cgggttttgt ccgcggcgga gcgccggggg cggagaagat ttcagcgtca
     3421 gagcggcgga tgtagttgcc gtcgaggagg aggggatcgt cgaagtcgcg ggcctgaagg
     3481 cggg
//
LOCUS       DASZYN010000460         3695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_854308, whole genome shotgun sequence.
ACCESSION   DASZYN010000460 DASZYN010000000
VERSION     DASZYN010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854308"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VHE33_11860"
     CDS             complement(<1..316)
                     /locus_tag="VHE33_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98193.1"
                     /translation="MNSRPTPTRVAYFPDSFHEINGVAHTSRHFEEFARRRKLPFLCV
                     RAGERAESVATDGALTTLELPRGILSIPLDKDLKFDPAWIRHLPAIARVVRDFRPDVI
                     HIT"
     gene            complement(332..1501)
                     /locus_tag="VHE33_11865"
     CDS             complement(332..1501)
                     /locus_tag="VHE33_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98194.1"
                     /translation="MASLRRISYLWKEPDAAGTYSTGISLHSHTNQSKETLDFLANLG
                     SQYPLLRPILARSERKSRERHSIPINYAAAYWTPPLTPRIAFDVESGQIEKKLHLMPL
                     VSITDHDTIAAPMLLRTVAAARHIPVSVEWSAPFGGDQAFHLGIHNLPSDQGAMWMKT
                     FAEYTAKPDEKRLTEILAALHELPNVLIVFNHPMWDLYLIGAEKAAQRVQDFLAANHE
                     FMHAFELNGLRPWEENRSVKRLAQQWNKLLISGGDRHGMEPNANVNLSRATSFTEFVH
                     EIRYQDRSHVLFMPQYSEPWKHRILESTLDAIRDYPEFPTGSRYWDERVWHPDRNGVV
                     RPLRELWPDGTAPGWMTMTIKAVRLMGSSPVSGGLRMAWSESRELRFALGEDAIR"
     gene            complement(1740..2663)
                     /locus_tag="VHE33_11870"
     CDS             complement(1740..2663)
                     /locus_tag="VHE33_11870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HVT98195.1"
                     /translation="MPRKLPDPLTIAALAYENEAFLNSPDGRILRILAEYSEPLARFR
                     RERIQDTVVFFGSARFRALDEANRELELLENTGSRQPAPEEEQPAREGEAETSELRRR
                     RAEAAVEMAHYYEDARRLAFMLTKWAKDLKFRRNRFVVTSGGGPGIMEAANRGAWEAG
                     GKTIGLNIRLPFEQVPNRFITPALNFEFHYFFMRKYWFAYLAKALVVFPGGFGTLDEM
                     FEILTLSQTQKLAKKITVVLYGSQYWQQVINLDVLVDKGAISPRDRELFQPVDSTEEA
                     FEILRDGLTKNHLEPESAREAELPDIAETRR"
     gene            complement(2665..3063)
                     /locus_tag="VHE33_11875"
     CDS             complement(2665..3063)
                     /locus_tag="VHE33_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98196.1"
                     /translation="MLLLGKGRRAHFARLRALRVMPSQPNLVSPAPSPSASPAVTGGP
                     PEQRQTDDRSTHWLIRADLFLRVIVQLYVGIALVFLPWMHAWLYNRFFLYYAPVARVM
                     EHGAVRGLVSGLGLLNLWIAISEAIRYKES"
BASE COUNT          739 a         1137 c         1123 g          696 t
ORIGIN      
        1 cggtgatgtg aataacgtcc ggacgaaaat cccttaccac ccgagcaatg gcaggcaagt
       61 gccgtatcca cgccggatcg aacttcaggt ccttgtccag cgggatcgac agaatgccgc
      121 gaggcagctc aagagttgtc agcgcaccgt ccgtcgccac cgattcagcc cgctctccgg
      181 cacgcacgca gaggaaaggc agcttgcgcc gccgggcgaa ctcctcaaag tggcgcgacg
      241 tatgcgccac gccattgatc tcgtgaaatg aatcggggaa gtaagcaacg cgcgtcggag
      301 tcgggcgtga gttcaagccg caggcgcctc gttaacgaat cgcatcctcg cccagcgcga
      361 agcgcagttc gcgcgactcg ctccaggcca tgcgcagacc gcccgaaacc ggactgctgc
      421 ccattaaccg caccgccttg atggtcatcg tcatccatcc gggcgccgtg ccatcgggcc
      481 acagctcgcg cagcgggcga accacgccat tccgatccgg atgccacacc cgctcatccc
      541 agtagcgcga gccggtcggg aactccggat agtcgcggat ggcatcgagc gtcgattcga
      601 ggatgcgatg cttccacggc tccgagtact gcggcataaa gagcacgtgg ctgcggtcct
      661 ggtagcgaat ctcatgcacg aactcggtga agctcgtcgc acgcgaaagg ttgacgttgg
      721 cgttcggctc catgccgtgg cgatcgccgc ccgagatgag cagcttgttc cattgctgcg
      781 ccagacgctt cacactgcgg ttctcttccc acggacgcag gccgttcaac tcgaaggcgt
      841 gcatgaattc gtggtttgcc gccagaaaat cctggacccg ctgcgccgcc ttctctgcgc
      901 caatcaggta aaggtcccac atcgggtggt tgaagacgat cagcacgttc ggcagctcgt
      961 ggagcgccgc cagaatctcc gtgaggcgct tttcatcggg cttcgccgtg tactcggcga
     1021 aagtcttcat ccacatcgcg ccctgatcgc tgggaaggtt gtgaatgcca aggtggaaag
     1081 cctgatcgcc accgaagggc gcgctccact ccaccgatac aggaatatgc cgggcagcgg
     1141 ccaccgtacg cagcagcatc ggcgccgcga tcgtatcgtg gtccgtgatc gaaaccagcg
     1201 gcatcaggtg cagcttcttt tcgatctgtc cgctctcgac gtcgaaggcg atgcgaggcg
     1261 tcagcggcgg ggtccagtag gcagcagcat agttaatcgg tatgctgtgc cgctcccgcg
     1321 acttccgttc gctgcgagcg aggatgggac ggagcagtgg gtactggctg ccgaggttcg
     1381 ccaggaaatc cagtgtctct ttggactggt tcgtgtggct gtgcagcgag atcccggtcg
     1441 aatacgttcc agcggcgtca ggttctttcc agaggtagga gatgcggcgc agactggcca
     1501 tgggtggagc cctcctgggt cgaacttccg gcctcagtat ccgggctgca ccatgaatgg
     1561 ggcattacgc ggggaagaat gatcgaacaa atcagagtcc gcgccgggag ggtaacgtta
     1621 ctccagagat gccgtccagc tcataagccg ttgcagatca accatcttct cgtcggtacc
     1681 gtgcgcagag ctgtggtaac tccctcgata cggtacccgc gatcttctgt tacaactcgt
     1741 taacggcgag tctccgcaat atcgggcagc tctgcctcac gtgccgattc cggctcgaga
     1801 tggttcttcg tgagtccatc acgaagaatc tcaaacgcct cctcggtaga gtcaaccggc
     1861 tggaacagct cgcgatcgcg cggtgagatc gcgcccttgt cgaccaggac atcgagattg
     1921 atgacctgct gccagtactg cgaaccgtaa agcaccaccg tgatcttctt cgccagcttc
     1981 tgcgtctgcg agagggtcag gatctcgaac atctcatcga gcgtgccgaa tccgcccgga
     2041 aagaccacca gcgccttcgc gaggtacgcg aaccagtact tgcgcatgaa gaagtagtgg
     2101 aactcgaagt tgagcgccgg cgtgatgaac cggttcggca cctgctcgaa ggggagcctg
     2161 atgttcagcc cgatggtctt tccgccggct tcccacgcgc cgcggttggc cgcctccatg
     2221 atgccgggtc cgcctcccga agtaacgacg aagcgattgc gccggaactt aaggtccttg
     2281 gcccatttcg tcagcatgaa ggcgagacgg cgcgcgtcct catagtagtg ggccatttcg
     2341 acggcggcct cggcgcgccg ccgacgcagc tcgctggtct cggcttcacc ctcacgcgcc
     2401 ggctgttctt cttccggagc cggctggcgg gaaccggtgt tctcaagcag ttccagttcg
     2461 cgattcgcct catcgagcgc gcgaaaccgc gccgagccaa agaagacgac cgtatcctgg
     2521 atacgctcac ggcggaaacg ggccagcggc tcggagtatt cagccaggat gcgcaggatc
     2581 cggccgtccg ggctgttgag gaaggcctca ttttcgtagg ccagggcagc aatggtcagc
     2641 gggtccggaa gctttcgtgg catttcagct ttccttgtag cggatcgctt cggaaatggc
     2701 gatccagagg ttgagcaggc caaggccaga gaccagaccg cggaccgctc cgtgctccat
     2761 caccctggcc actggagcgt agtaaaggaa gaagcgattg tagagccacg cgtgcatcca
     2821 gggcagaaag accagggcga tgccgacgta gagctggaca atcacccgaa ggaacaggtc
     2881 ggcacggatg agccagtggg tgcttcgatc gtcggtctgg cgctgttccg gagggcctcc
     2941 cgtcacggca gggctggcag acggagaggg agcgggggac accagattgg gctgcgacgg
     3001 cataacgcga agagcgcgga gacgcgcgaa atgcgcgcgg cggcctttcc ccaacagtaa
     3061 catagcgatc ccgcgggggg cgggaccatc gccgttattg catggggagt cggaggcata
     3121 cggtggtggt acctgcttgg gcttgcattc gatgcattgg ttggtcgatt ccggcgaaag
     3181 ggcgcgcgta gctcctcagt gcggtgtccc gaaagtaagc cgaataaatt gccatcccga
     3241 ccggaccctg agcgcagcga aggaggagcg gagggacctg ctttttcttc catttcagac
     3301 gtcacccatc actggagcac gtaactggag ttatggaacg aacttcaggg acactacatg
     3361 agccccttgg aacattcgcc caacacctgc tcaaaaccac catgctccaa acccgctcac
     3421 agcttttcgc gaaccgctaa cagtttttag cgaaccggac ctcagccaac cgcttcaccg
     3481 gccgacccct ctaccagctg ccgcaagtga ccttcgtacc gtccaacact tcgattcttc
     3541 ttgaattgcc ccaggccact cgctaacctg aagacaatcg atcagtgtgt gaagagcccg
     3601 ccaccagcgc gggcttttct catggtgcgg gtttcgtttc cgcacctgac ccggcccagg
     3661 ccggatcgag tccgttcttt gaaaacccag cctcc
//
LOCUS       DASZYN010000461         3002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_471903, whole genome shotgun sequence.
ACCESSION   DASZYN010000461 DASZYN010000000
VERSION     DASZYN010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471903"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VHE33_11880"
     CDS             complement(<1..525)
                     /locus_tag="VHE33_11880"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HVT98197.1"
                     /translation="MLAWINFITRLLNEWFGAPVTALLEKLGIHPPHPSHPINNTTTL
                     ELIVLAGLILFFIVVRLRLSVEKPGNAQHLAEMVHEFVSGQAGQVMGHGFEKFLPVVT
                     CLLLFIVCCNCFGLFPGIETPTANPAVPLGLAIFTFLYYHWHGLRAQGPIGYLKHFMG
                     PVWWIAPLMFPIEII"
     gene            complement(536..961)
                     /locus_tag="VHE33_11885"
     CDS             complement(536..961)
                     /locus_tag="VHE33_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98198.1"
                     /translation="MSPGTDPVFNVAGSMSDEDLQQLLRRATRTVVILALLLFAVFTF
                     TMGWQSGLLELAGAVISYTGIREWRTLARLVSATMDNRPAPSPKGRTLVMFFLRLALV
                     GGILYVSLRCLNGSVYALVAGIGLAVVALTIEALRLLRQ"
     gene            complement(958..1236)
                     /locus_tag="VHE33_11890"
     CDS             complement(958..1236)
                     /locus_tag="VHE33_11890"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HVT98199.1"
                     /translation="MPPDSPRPQSETPKRSALTRGVAGYERIMEIAFIMPAALVVGWL
                     IGAGLDKLFHQHWIYIAGIVFGFVAGFIEVLRSAIRYSKKSEQEDRGT"
     gene            1331..2974
                     /gene="lysS"
                     /locus_tag="VHE33_11895"
     CDS             1331..2974
                     /gene="lysS"
                     /locus_tag="VHE33_11895"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HVT98200.1"
                     /translation="MSHDVFESEFERSLYSLRQEKLKQLDGLGQKAYPNTFAPPADHP
                     LTAIPKIRAQYDTWIGEQFEATRVPIAVAGRIMAIRQQGKAGFATLQQDGARLQIYVR
                     KDAVGDQAFELYKLLDLGDHIGVTGYLFRTRTNELTIHVETITFLAKALLALPEKYHG
                     LSDIELRYRQRYVDLFMNGVLNEAPAEGATEAQKAEEIHVRDVFVKRAAVLRAMRRFF
                     DARGYIEVETPMMQPIAGGAAAKPFMTHHNTLDMDLFLRIAPELYLKRLVVGGFDRVY
                     EINRNFRNEGISTQHNPEFTMLEFYQAYANYKDLIELTKELIVYVAKEVNGTTVTHFN
                     GVEIDLAKWRTFTMREAIIEFWPEAVGPKPTAADFESEEAVARLSERAGTVHFIERKP
                     DEPRGKTIASIFEAVAEEHLIQPTIIYEFPTVVSPLSKQKPDEPDWVERFEFYIGGFE
                     LGNAFSELNDPEEQFRRFKDQLEQHERGDEEAHQLDVDYVRALAYGLPPTAGEGIGID
                     RLTMILTGARSIRDVILFPLLRPQAKEPEAGGGKQESPN"
BASE COUNT          673 a          845 c          958 g          526 t
ORIGIN      
        1 aatgatctca atggggaaca tcaggggggc gatccaccac acgggcccca tgaaatgctt
       61 cagatagcca atcggaccct gagctcgcag cccgtgccag tggtagtaaa ggaacgtgaa
      121 gatggccagt cccagcggaa ctgccgggtt ggcggttggg gtctcaattc cggggaacag
      181 cccgaagcag ttgcagcaga cgatgaagag cagcaggcac gtcaccacgg gcagaaattt
      241 ctcaaagccg tgtcccatga cctgtccagc ctgcccactg acgaactcgt ggaccatctc
      301 ggccagatgc tgggcgttgc ccggcttctc gacgctgaga cgcagtcgca ccacgatgaa
      361 gaacagaatc agcccagcga gaacgatcag ttcgagcgtg gttgtgttgt tgatcggatg
      421 gctggggtgg ggagggtgaa tgccgagttt ttcgagcagc gccgtgacag gcgcgccgaa
      481 ccactcgttc agcagacggg taatgaagtt gatccaggca agcatgcgtt ttcttctatt
      541 ggcgaagcag gcgaagggcc tcgatggtaa gggcaacgac ggctaatccg atgcccgcga
      601 ctaaggcgta aacactaccg ttcaaacacc ttagactaac atacagaatc cccccgacga
      661 gcgccaaacg aagaaaaaac atgaccaaag tgcgcccttt tgggctgggg gccggccgat
      721 tgtccatggt ggcggaaacc agcctggcca gggtgcgcca ctcacggata ccggtatacg
      781 agattacggc tccggccagt tccagaaggc ccgactgcca gcccatcgtg aaggtgaaaa
      841 ccgcgaaaag caggagggcc agtatcacca ccgtgcgggt tgcgcgccgg agcaattgct
      901 gcagatcctc gtcgctcatc gagccggcca cgttgaagac cggatccgtg ccggggctca
      961 tgtgccgcga tcctcctgct ccgacttttt cgagtagcgg atcgcggagc ggagcacctc
     1021 gatgaagccg gcgacaaacc caaaaacgat cccggcaatg tagatccagt gctggtgaaa
     1081 caacttgtcg aggccggcgc cgatcagcca gcccaccacc agcgccgccg gcatgatgaa
     1141 ggcgatctcc atgatccgct cgtagccggc gactccccgg gtgagggccg agcgcttcgg
     1201 agtttcggat tgagggcgtg gagaatcggg tggcatgtgg cacttcccgc cttgttatac
     1261 accagcagcg cggcaagtcg cggcgaacgc taaagagcgc aaaggctgaa agttatactc
     1321 gaacgagagc atgagccacg acgtattcga atccgagttt gagcgaagcc tttattcgct
     1381 tcgccaggaa aagctgaagc agctcgatgg tctcgggcag aaggcttatc ccaacacctt
     1441 cgcgcctccg gcggaccatc cgctcactgc gattccgaag atccgcgcgc agtacgacac
     1501 ctggatcggc gaacagtttg aagcgacgcg tgtgcccatc gcggttgccg ggcgcatcat
     1561 ggccatccgg cagcagggca aggccggctt tgcgacgttg cagcaggatg gcgcgcggct
     1621 gcagatttat gtgcgcaagg atgccgtcgg cgaccaggcg ttcgaactgt ataagttgct
     1681 ggacctgggg gaccacatcg gcgtcacggg ttatctgttc cgcacgcgaa cgaacgaact
     1741 gacgattcac gtggagacga tcacgttcct ggcgaaggcg ctgctggcgt tgccggaaaa
     1801 gtatcacggg ctgagcgata tcgagctgcg gtatcggcaa cggtacgtcg atctgttcat
     1861 gaacggcgtg ctcaatgaag ctccggctga gggtgcgacc gaagcgcaga aggccgaaga
     1921 aattcatgtg cgcgatgtgt tcgtgaagcg cgcggcggtg ctgcgcgcga tgcggcggtt
     1981 cttcgatgcg cgcggataca tcgaagtcga gacaccgatg atgcagccga tcgcgggtgg
     2041 agcggcggcc aagccgttca tgacgcatca caacaccctc gatatggacc tgttcctgcg
     2101 catcgcgccg gagttgtatc tcaagcggct ggtggtgggc ggcttcgatc gcgtgtacga
     2161 gattaatcgc aacttccgca acgaaggcat ctcgacgcag cacaatcccg agttcacgat
     2221 gctggagttc taccaggcct acgcgaacta caaggacctg attgagctga cgaaagagct
     2281 gatcgtgtac gtcgcgaagg aagtgaacgg gacgacggtg acgcacttca acggggtcga
     2341 gattgatctg gcgaagtggc gcacgttcac gatgagggaa gcgatcatcg agttttggcc
     2401 ggaagccgtg ggcccgaagc cgacggcggc agatttcgag agcgaagaag ccgtagccag
     2461 gctctcagaa cgcgcaggga cggttcactt catcgaacgc aagccggacg aacctcgcgg
     2521 caagaccatt gcttcgattt ttgaggccgt ggccgaggag catctcatcc agccgacgat
     2581 catctacgaa tttccaaccg tggtttcccc gctttcgaag caaaagcccg acgagccgga
     2641 ctgggtggag cgcttcgagt tctacattgg cggattcgag ctcggcaatg ccttcagcga
     2701 gctgaacgat cccgaagaac agttccgtcg cttcaaagat caactggaac agcacgagcg
     2761 gggcgatgag gaagcgcacc aactggacgt cgactacgtg cgggcgctgg cttacgggct
     2821 gcctccgacg gcgggcgagg ggatcgggat cgaccggctg accatgatcc tcacaggcgc
     2881 gcgttcgatt cgcgatgtga tcctgttccc gctgctccgg ccgcaggcga aggagccgga
     2941 agccggtggc gggaagcagg aatcaccaaa ctaggtctgg cggggacccg aagaagttac
     3001 gc
//
LOCUS       DASZYN010000462         2949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_471963, whole genome shotgun sequence.
ACCESSION   DASZYN010000462 DASZYN010000000
VERSION     DASZYN010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471963"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VHE33_11900"
     CDS             <1..306
                     /locus_tag="VHE33_11900"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HVT98201.1"
                     /translation="MRSVVPPDILNPLDQLEHDHREADQLHDDIDRLYSDWLSSGTLD
                     PERQEAMLHKTSRLRALYSEHIHTEEATVFPAAARVLDRDALAAIGSEFRSRRNPVS"
     gene            531..2222
                     /gene="aroE"
                     /locus_tag="VHE33_11905"
     CDS             531..2222
                     /gene="aroE"
                     /locus_tag="VHE33_11905"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HVT98202.1"
                     /translation="MKAATAVPRTAPPRDGGKSGIPKGSAPRPASPGQRASSASGNGT
                     AAVETPAFTAHYIRSRIGKICVAITGSTPAEMLENAERIVRENHFVEFRLDYLANPVA
                     ILPRLKQFLYERGEITAIATCRRSATGGKFKGTIGAELEVLEKSAAAGVHLIDIELQT
                     AEHMKPAQLKKLRSHGAALIISWHDFEGTKNLDAILDRILPYQPEFAKIVSTAKTLSD
                     NVAMMRFLDRRRDETNLIGISMGEQGVISRVLGLRAGSVFTFASALPGEETGPGQIDA
                     RTLRETWRIDQIDQSTKVYGVAGNPVRHSLSPLIHNMAFRRETVNAVFLPLQTSRLSD
                     LLTLTREVPLHGLAITMPFKTEILKHLERTDALSEKIGACNTVVRAQDGKLYGFNTDV
                     AAVIRPLERRLPLRGAKILVLGAGGAGRAAVFGLVDKGAEVLIWNRSADTAKKLARQA
                     KAKIIRREQIAKTNFDVIINTTPVGMKGVKPASILEPKELNTRLVFDLVYNPIDTPLV
                     RMAREKGLPVITGVEMFVHQGARQFEIWSGKPAPEEEMLRVVVHALRQESAKQQG"
     gene            2411..2770
                     /locus_tag="VHE33_11910"
     CDS             2411..2770
                     /locus_tag="VHE33_11910"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HVT98203.1"
                     /translation="MPIKIEDGTDDIVEIRAIGKLTKEDFTAFVPEFEGRVRRLGKLR
                     VLFDITSFDGWQPDGIWEEIKFDWKHNSDYRRLAVVGDEKWQHLLVTAFKPFTFAEVR
                     YFSLPQSDQARQWLLRP"
BASE COUNT          620 a         1043 c          751 g          535 t
ORIGIN      
        1 atgcgcagcg tcgtgccccc tgacatcctg aatccgctcg accagctcga acacgatcac
       61 cgcgaagccg accagcttca tgacgacatc gaccgcctct actccgactg gctctcctcg
      121 ggtaccctcg accccgaacg ccaggaagcc atgctgcaca aaaccagccg ccttcgcgcc
      181 ctctactccg agcacatcca cactgaagag gcgaccgtct ttcccgccgc tgcccgcgtg
      241 ctcgaccgcg acgcgctggc tgccatcggt tcggagttcc gctcccgccg caaccccgtt
      301 agctaaaggt ccaaactcgc cgggtggggt cctgtcagcc cggccagctg cccagttcac
      361 gctgacagct ataacttagc tccctttttt caataatttg cctcacgttc aagctgtttt
      421 ccacgaatcg atccccgcct gtcggcagaa atccgtccac caaaagcctc ccgcgcgcaa
      481 tagctgttgc atcgcaacgc aaaccccttt aatatgcgtc aattgcgggt atgaaagcag
      541 ctacggcggt cccgcgcacc gcacccccac gcgacggcgg caaatccggt atcccgaagg
      601 gctcggcccc ccgtcctgca tcgccagggc agcgggcaag ttctgcctcc ggcaacggca
      661 ccgccgctgt cgaaacaccc gccttcaccg cgcactacat ccgctctcgc atcggtaaga
      721 tttgtgtcgc catcaccggc tctacgcccg ccgagatgct cgaaaacgcc gagcgcatcg
      781 tccgagagaa tcacttcgtc gaatttcgcc tcgactatct cgccaatccc gtcgcgatcc
      841 ttccgaggct aaagcagttc ctctacgaac gcggcgagat caccgccatc gccacctgcc
      901 gccgctccgc caccggagga aaattcaaag gcaccatcgg cgccgaactc gaggtcctcg
      961 aaaaatccgc cgccgccggc gttcacctca tcgacatcga actgcaaacc gccgaacaca
     1021 tgaagccggc gcagttgaag aaactgcgct cccacggcgc cgcactcatc atcagctggc
     1081 acgactttga gggcactaaa aacctcgacg ccatcctcga tcgcatcctg ccttaccagc
     1141 ccgagttcgc caagatcgtc tctactgcga agaccctctc cgacaatgtg gccatgatgc
     1201 gcttcctcga tcgccgccgc gacgagacca acctcatcgg catttccatg ggcgagcagg
     1261 gcgttatcag tcgcgtcctc ggcctgcgcg cgggcagtgt cttcactttc gcctccgccc
     1321 ttcccggcga agaaaccggc cccggccaga tcgacgcgcg taccctccgc gaaacctggc
     1381 gcatcgatca gatcgatcag tccacgaagg tctatggcgt cgcaggcaat cccgtccgcc
     1441 actcgctctc gccgctcatc cataacatgg ccttccgccg tgagaccgtc aacgccgtct
     1501 tcctcccgct gcaaacctcg cgcctcagcg atctgctcac gctcacgcgc gaagtcccgc
     1561 tccacggcct cgccatcact atgccgttca agaccgaaat tctgaaacat cttgaacgca
     1621 ccgacgcgct ctccgaaaag atcggcgcct gcaacaccgt ggtccgcgcg caggatggca
     1681 agctctacgg cttcaacacc gatgtcgccg ccgtcattcg cccactggag cgtcgcctac
     1741 cgttacgcgg ggcgaaaatt cttgtcctcg gagcgggtgg cgctggacgt gccgctgtct
     1801 tcggcctggt cgacaaaggc gccgaagtgc tcatctggaa ccgctccgcg gacaccgcaa
     1861 agaaactcgc gcgtcaggcg aaggcgaaga tcatccgtcg cgagcagatc gcgaagacca
     1921 actttgacgt catcatcaac accacgccgg tcgggatgaa gggcgtcaag cccgcgtcga
     1981 tcctcgagcc caaagaactg aacacccgcc tcgtcttcga cctcgtctac aaccccattg
     2041 atacgcccct ggtccgcatg gcccgcgaaa aaggcctgcc cgtcatcacc ggcgtcgaga
     2101 tgtttgtcca ccagggcgcc cgccagttcg agatctggtc cggcaagccc gcccctgaag
     2161 aagaaatgct ccgcgtggtg gtgcacgcat tgcgccagga aagcgcgaag cagcagggct
     2221 gagcgtcatc ttccttaagc tgtcattctg acgtcgcgca tcgcgcggcg gaagaacctg
     2281 cggttgtttt accgcattct tgcgtcccca acatcggggg agagcggtcg atgcgtactg
     2341 ggacacatct cccgccgtgg tcctctcagc gcatctaaaa aagcagcaac tgaatcaagg
     2401 aggtttctgc gtgcccatca aaatcgagga cggaaccgac gatatcgtgg agatccgcgc
     2461 aatcggaaaa ctgaccaaag aggactttac tgccttcgtc cccgaatttg agggccgtgt
     2521 ccgcaggctc ggcaaactgc gcgtcctctt cgatatcacc agctttgacg gctggcaacc
     2581 tgacggaatc tgggaagaaa tcaagttcga ctggaagcac aacagcgatt accgtcgtct
     2641 ggccgtagtt ggcgacgaga aatggcaaca tctgctcgtt accgcgttca aaccctttac
     2701 gttcgctgaa gtccgatatt tttcgctgcc ccagtccgat caggccaggc aatggctatt
     2761 gaggccctga cgatcgccgt cgcagtctcc gaacggctcg ctcctgcgag tgcgccaacc
     2821 acccttcggt aatctgtccc accccatcca cagaccgtag cctgaactta cctctctgcg
     2881 aagtgcaaag cggaaacgcg aagattggcc agaaaaaggc caaacggctc gcgaaccgcc
     2941 ataacggtc
//
LOCUS       DASZYN010000463         7983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1240760, whole genome shotgun sequence.
ACCESSION   DASZYN010000463 DASZYN010000000
VERSION     DASZYN010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7983
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240760"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            458..7732
                     /locus_tag="VHE33_11915"
     CDS             458..7732
                     /locus_tag="VHE33_11915"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HVT98204.1"
                     /translation="MSSIAAGSVITLTARVNAGATAVNPGQVKFCDSSATSCADIHLL
                     GTAQLTTAGTATFKLRPGIGSYSYTAVFMGTNKYSSSTSVTMQLTATGTIPSLATSAT
                     INQAGSWGAYTLSATVTETGNTAAPSGSVSFLDTNHGNAVLGTGTLGAATRGVAWSAV
                     SSSAPTVAGVSWLVTDLNDDGIPDLFVKDYFGTYDVLLGKGDGTFTVLGSPFGPSSGI
                     GSFVVGDFNNDGIPDVAAVDGQYLALTSSITIFLGNGDGTFTVAGTSPALGYNPTAIT
                     TADINGDGNADLVLSEQESESSGNGQVLIFWGNGDGTFTQASSSVSLASVASSILPAD
                     LNGDGNVDLVMCGAGPSGTILLGRGDGTFTSVTGPGAAINGSTIAVADLNSDGIPDLV
                     FETSHLTVFLGNGDGTFTEVSAGANDTLTIGGFAVGDFNQDGIPDIVYDIPGTVRIGM
                     LFGQGDGTFIQSTGTANYAYDFSGVLAVADFNGDGWPDVLTEDGNSRTFIDALTQPTE
                     TATALATVSIAVAVTHLGDASYPGDSNYNPTVSGTLPLWGAPPATTTALTVTSGGAPV
                     SSVPSGTAVTLTSTVVSGASPIAAGQVNFCDATATSCSDIHLLATVALSSNGTAAFNL
                     VPGPGTHSYKAVFVQNGYGLTSSSAPTLLTVGSAPPVMYSDTTAVSASGSPGDYSLTA
                     TVTGYGGSAVPTGTVSFLDTSFGNASLGTAQLGSGVAGLGWLISQTPAFGTVPISEVT
                     ADFNGDGIPDVAVLSSNSSSGGPYTIAILQGKGDGTLSAGTSFATGLSASQSAQSMIV
                     GDFNGDAKTDIAILSYDYSFDTSVTIFAGRGDGTFGSGQTSPAYTQPNTGGDFYSGMM
                     AAADFNGDGNLDVAVSGDCINDCGITVLLGNGDGTFKAGMTFAVTQDFGLIATGDFNG
                     DGVTDIVATNYFEFGGSPTIFLGKGDGTFTPKETSFTLDYFPPSLVVGDFNGDGSLDL
                     AFSDLNGVEIALGKGDGTFTETPASPMAVPSEIYSLKAGDFNHDGKLDLAGVDSYNDK
                     IVLLIGAGDGTFAVTATTPAVSTVWLGPFAIAAADFNEDGIPDLAMLTKNQATASILI
                     TSPTETASATLGDVAPVGAGTHNVEASYPGDSNYPSAISAPVALDAGLKPVIVTPAGG
                     TFSSIVTVTMTEAIPGATIYYEASGAFTTNGWVQYTAPIVMSIGGQETITAYATETGY
                     SQSNLATAAFNLNFPVAPAPIISPAGGVSPGSQTVTITDPASAATIYYTTDGTSPTTS
                     SAVYLQPITISTSETVAAMAVGGGYSASPSVSAQFFIQSSQSRFIYTIAGSGYRGFAG
                     DGGPAPLAVLNGPSNTAVDSAGNLYIADGGNRVVRKVEVASGTISTVAGTGTAGYSGD
                     GGPATSAQLYYPNSLALDTAGNLYISDSGNGVIRRVDARTGNIATYAGNSTATTIGDG
                     GPAANAKLTYPQGIAFDSAGDLYIGEQTRVRVVNANTGIISTVAGNGTYGFSGDGGAA
                     TSAQLTSVAGIAFDQSGNLYLADSSNNVIRKVMAATGIITSVAGQGGPSGRGYSGDGG
                     PATSARLSGPQGVTVDSAGNLYIADTYNYVLREVTAVDGTISTISGHPNSCSSLSGDG
                     GPAVESALCYATGVTVDGHDNLYIAESGISRIRKITLPIVPPSAATAQPSFSVAAGSY
                     VSPQTLTVSDATPGAEIYVTINGTQAIPVGQGYFGPIGITGSASVQAVAVAPGYLPSD
                     AASAVYTITTPPTAIIDTFAGNGTTGFSGVGGPAIDAGLVSPEGLAFDSTGSLYIADP
                     DAAVVWMVSATTGNITVAAGVPGVHSFQSPSGPATSTPLYTPQQVAVDTAGNLYISDQ
                     QLQRVLRVDAKTDMMTVYAGSGNPPSIGDGGPATQAWISPAGIAFDQAGNLYIADTAN
                     GRIRKVSAATGIISTVAGGGTNGLGDGGPATSAVLSDPGPIVFDSKGNLFVGDLNTAR
                     VRVVDAKTGIITTFAGNGNNGGTGDGGQATTAEIIPEGLAIDSADNLYISNTLGGIRM
                     VPAGGGLITRVIGVPFAGFGGDGGAASMAEFCAPDGLAFDKTGSLYIADSCNNRVRKV
                     TYPAPAATPTFSVASGTYAEAQTVTITDATQDAAIYYTTDGSVPTTGSTLYNAPLTVA
                     SSETVKAIAVAAGYTVSAVASANYVIGNQKAVPDVGVVKSSLNPSMTGTAVTFSVTIS
                     SGLGSPSGTVTFMDGAAQLGSGTLTGGSANYTTSALAAGSHSITAVYSGDVNFTSVTS
                     TALTQVVESFSIALPGGSSSTATASPGGQAKYTFVITPPATGSALTFAVTGLPAGATG
                     TFSPATVAAGAGVTNVTMIVTLPATIAMQSDEQPFGRGALALSFGLVLLPFARRIGRG
                     WRNLPSLLLLIGIGVTFCFGVSACGGSGPKQSPSPQTYTLTVTASSGTLAQSTTVTLI
                     VQ"
BASE COUNT         1661 a         2310 c         2335 g         1677 t
ORIGIN      
        1 ggcgggttcg cggctcagac cacagaatca gcgcaagtat gaaggcaact gttacgttac
       61 cgagcccgca cctgcactcc ctcgaaagat acaccgtcgg cgtcagcatc tttcgttagc
      121 ggatatcgta atggcatatg ctatgattac acgtttccaa ataattaggt tgcaaccttg
      181 ccctgcatat gacgggatcg agcttgcgtt cattgatatg cgtgccacgt gcttgtgcta
      241 atgggagata cctgtgtcgt ctttttgttg ccttgatacg aaaccaatcc tctctggcct
      301 tttcaggccg ataccctgta agcagaagcg caggccgcaa ttagcgctga catgcctttt
      361 gctggcggca ttctcctgcg ccatacccga aacagcggcg gcgcaatcga agacggcaac
      421 cctcacgagc ctcgcaatca cttccggggg aaatccggtg agttcgatcg ccgcggggag
      481 cgtgataacc ctcacggcgc gggtgaatgc gggggcgacg gcggtgaatc ctggacaggt
      541 gaaattctgc gacagttcgg ctacgagctg tgccgatatt cacctactgg ggacagcgca
      601 gctaacgact gcagggaccg ccactttcaa gcttcgcccg ggcattggga gttacagtta
      661 caccgcagta tttatgggta cgaacaagta ttcctcaagt acttcggtaa cgatgcagct
      721 gacggcaacc gggacaattc catctctcgc tacgtctgca acgatcaatc aggctggaag
      781 ctggggagca tatacacttt cagccaccgt gacagagacc ggtaatacgg ctgcgcccag
      841 cggttctgtc tcgtttctgg acacgaacca cggaaatgcg gtgcttggca cgggaacgct
      901 cggcgctgcc acgcgcggtg tggcgtggtc agccgtcagc agcagcgccc caactgttgc
      961 cggcgtctcc tggctggtga cggacctgaa cgacgacgga attccggatc tattcgtcaa
     1021 agattacttt ggcacctatg acgtgctgct cggcaagggg gatggaacct ttacggtact
     1081 gggctcgccc ttcggcccgt ccagcggaat cggttcgttt gtggtgggcg acttcaacaa
     1141 tgacggaatt ccggatgttg ctgcagttga tggtcagtat ctcgcactga ccagctccat
     1201 aactatattt ctcggtaatg gtgacggcac gtttacggtg gcagggacca gtccggccct
     1261 tggctataac cccaccgcga ttacgacggc agatatcaat ggggatggaa atgccgatct
     1321 ggttttgagc gagcaggagt cggagtcttc aggcaacggg caagtcctga tcttttgggg
     1381 caacggcgat gggacgttta cgcaggcgtc atccagcgtt tcattagctt cggtagcctc
     1441 ttcgatcctt ccagccgacc tgaatggtga cggcaacgtg gatcttgtaa tgtgcggggc
     1501 ggggccgagc gggacgattc tgctaggccg gggcgatggg acttttacga gtgtgacggg
     1561 cccaggtgcg gcgatcaacg ggtcgaccat tgcggttgcg gacctcaata gtgatggcat
     1621 tcctgatctg gtctttgaga cttcccatct gacggtcttc cttggaaatg gcgatggcac
     1681 attcactgag gtgtccgccg gtgcgaatga caccctgaca atcggaggat ttgccgtagg
     1741 agacttcaac caggatggaa ttcccgatat cgtttacgac atccccggca ccgtcagaat
     1801 cggaatgtta ttcggacagg gtgacgggac cttcattcaa tcgactggca ctgcgaacta
     1861 cgcatacgat ttcagcggcg ttcttgctgt tgcggacttc aacggagatg gctggcccga
     1921 tgtgcttacc gaggacggaa attcacggac gttcatcgat gcgctgactc agcccacaga
     1981 aaccgcaacc gcgttggcga cggtgtcgat cgccgtcgcg gttactcatc ttggagacgc
     2041 cagttaccca ggtgactcga actacaatcc gaccgtctcc ggaactcttc cattgtgggg
     2101 agcacctccg gcgacaacga ctgctctgac cgtcacttcc ggaggagctc cggtctcatc
     2161 ggtgccttcc ggaacagcgg ttacgcttac ttcaacagta gtctccgggg ccagtccgat
     2221 tgccgcgggc caggtcaatt tctgcgatgc tactgcgact tcgtgctccg atatccatct
     2281 gctggcaacc gttgctctgt cgagcaacgg aactgcagca ttcaacctgg tgccgggacc
     2341 cggaacacac agctataaag ccgtctttgt acagaatggc tacggtctta ccagttcatc
     2401 cgcaccaaca ttgctgaccg tgggctccgc tccacccgtg atgtactcag acacgactgc
     2461 ggtttcagcc agcggatctc ccggagacta ttccctgaca gcaacggtca ccggatatgg
     2521 cggatcggcg gtaccaacgg ggaccgtctc ttttttagat acgagcttcg ggaacgcctc
     2581 acttggaaca gcgcaactgg gaagcggcgt cgcgggtctc ggctggctca tatcccagac
     2641 gccagcgttt gggacggttc cgatttctga agtcacagcg gacttcaacg gagacggcat
     2701 accggacgtt gctgtgctct caagtaacag ttccagcggt gggccttaca caatcgcgat
     2761 tcttcagggt aagggtgacg gtacattgag tgcgggaaca tcattcgcca ccggactcag
     2821 cgccagtcag tccgcccaat cgatgatagt cggcgacttc aacggcgatg ccaaaacgga
     2881 tatagccatc ctcagctacg actatagttt tgataccagc gtaacgatct tcgccggcag
     2941 gggagacggc accttcggaa gcggccaaac cagcccagct tacacgcagc ccaacacggg
     3001 tggggacttc tattcgggaa tgatggccgc agccgatttc aacggcgacg gtaatctgga
     3061 tgtggcggta tcaggagact gcattaacga ttgcggcatc actgttcttc tggggaacgg
     3121 agatggcacg tttaaggctg gaatgacctt tgcggtaacg caggactttg gattgatcgc
     3181 gaccggggac ttcaatggcg atggtgttac ggatattgtc gcaacgaact acttcgagtt
     3241 cggggggagt ccaaccatct tccttggcaa aggagacggg acattcaccc caaaggaaac
     3301 ttccttcact ctggattact ttcccccgtc cctggtggtc ggagacttca atggggatgg
     3361 cagtctggat ctggcgttct cggacctgaa tggagttgag atagccctgg ggaagggaga
     3421 tggaactttc actgagacgc cggccagccc catggcggtc cccagcgaaa tctacagcct
     3481 caaggcggga gacttcaacc acgacggcaa gctggatctg gcgggagtcg acagctacaa
     3541 cgataagatc gttcttctca tcggagccgg agacggtacc tttgcagtta cagctacgac
     3601 tcctgctgtg agcacggtct ggcttggtcc ctttgcgatc gcagccgccg atttcaatga
     3661 ggacggcatt ccggatttgg caatgctgac caagaatcag gccacggcgt ccattctcat
     3721 cacctcaccg actgaaacag caagcgcgac cctgggcgat gtagccccgg tgggcgcagg
     3781 cacgcacaat gtggaagcca gttatcccgg agacagcaac tatccttcag ccatatctgc
     3841 accggtggcg cttgacgccg gtctcaaacc ggtaatcgtc acgcccgcgg gcggaacatt
     3901 ctcttcaatc gtgacagtca ccatgaccga agccattccc ggcgccacta tctactacga
     3961 ggcatccggc gccttcacca cgaatggctg ggttcagtac accgcaccga ttgtgatgag
     4021 cattggtgga caggaaacga tcaccgccta tgccacagaa accggctact ctcaaagtaa
     4081 tcttgcgaca gctgcgttca acctgaattt tccggtagcg cctgctccca tcatctcgcc
     4141 ggcaggcgga gtttcccctg gttcgcaaac tgtcacgatt accgacccag cgtcggccgc
     4201 taccatctac tacacgactg acgggacctc gcccaccacc agttcggccg tctatttgca
     4261 gcccatcacg atttccacat cggagacggt cgccgctatg gccgtcggcg gcggatacag
     4321 cgccagccct tcggtcagcg ctcagttctt catccagtct tcgcagagcc gcttcatcta
     4381 caccatcgcg ggaagcgggt accggggatt cgccggcgac ggggggccgg cgcccctggc
     4441 agtcctgaat ggtccatcca atactgccgt cgacagtgcc ggcaatcttt acatcgctga
     4501 cggcggtaat cgggtcgtac gcaaggtcga ggtcgccagt ggaaccatat ccactgtggc
     4561 gggcaccggg acggccggat acagtgggga cggcgggcca gcaacgagcg cgcaactgta
     4621 ttatccgaac agcctggctc tcgataccgc cggaaatctc tatatcagcg actcaggaaa
     4681 cggcgtcatc cggcgagtgg acgccaggac gggcaatatt gcaacctacg ccggcaacag
     4741 caccgccacc accataggcg acggtggccc cgcagcgaac gcgaagctca cttatcctca
     4801 gggcattgcc ttcgacagtg ccggagacct ctacattggc gaacagacgc gggtgcgcgt
     4861 ggtcaacgcc aatacgggta ttatcagcac ggttgcggga aacggaacgt atggtttttc
     4921 tggcgatggt ggcgctgcca ccagtgcaca gctgacttcc gttgccggca ttgcctttga
     4981 tcagtccgga aacctgtacc ttgcggactc atctaacaat gtcatccgca aggtaatggc
     5041 cgctactggt atcatcacgt ccgtagccgg gcagggcggc ccgtccggcc ggggatacag
     5101 cggcgatggc ggacccgcaa ccagtgcgag gctttccggc ccccagggag taacagtcga
     5161 ctccgccggc aatctctaca tcgctgacac ttacaattat gtgctgcgcg aggtgacggc
     5221 tgttgacgga acgatcagca caatttcagg acatcccaac tcgtgcagta gtttaagtgg
     5281 agatggagga ccggcagtcg agtccgcgct ctgttatgcg acgggtgtta ccgtggatgg
     5341 ccacgacaat ctctatattg ccgagagcgg aattagccgg atccggaaga taaccctgcc
     5401 catcgttcct ccgtcagcgg caacggcgca gccatcgttc agtgtcgcag caggaagcta
     5461 tgtgagccca caaacgctaa ccgtatccga tgcaactccg ggtgccgaga tctacgttac
     5521 gatcaatggc acgcaggcaa ttccggttgg ccaggggtac ttcggtccga tcgggataac
     5581 aggttccgcc agcgttcagg ctgtggcggt agcgccagga tacctgccca gcgatgcggc
     5641 ctcggcggtc tacacgatca ccacgccacc cacggcgatt atcgatactt ttgcaggtaa
     5701 cggcactacg ggattctccg gagtcggagg gccggcgatc gacgccggat tggtttctcc
     5761 tgagggcctt gccttcgaca gcaccggcag cctctatatc gccgatccgg acgcggcagt
     5821 ggtctggatg gtgtctgcta cgacaggaaa catcacagta gctgcgggcg tgccgggtgt
     5881 gcacagtttc cagtcgcctt ccggtcccgc taccagcacg cctttataca cgccgcagca
     5941 ggttgccgtc gatacagccg gaaacctgta catctccgac cagcaacttc agagagttct
     6001 ccgagtagac gcgaagacgg acatgatgac ggtgtatgcg ggtagcggca atccgccgtc
     6061 cataggagac ggagggccgg ccacacaggc ctggatttct cccgctggta tcgccttcga
     6121 tcaggccgga aacctctata tcgccgatac agctaatggc cgcatacgta aggtttctgc
     6181 cgctaccgga attatctcca ctgtcgccgg tggtggaacg aacggcctgg gcgacggagg
     6241 gcccgcgact tcggcagtgc tcagtgaccc tggaccgatc gtcttcgaca gcaagggaaa
     6301 tctctttgtc ggcgacctga acacagcgcg cgtgcgggtg gtcgacgcga agaccggaat
     6361 catcacgacg ttcgcaggta acggaaacaa tggaggaacc ggcgacggcg gtcaggcaac
     6421 cacggcggag atcattcctg agggtctcgc gatcgattca gcggacaact tatatatatc
     6481 gaatacgctt ggcgggatac ggatggttcc ggccggtgga ggcctgatca cacgagtcat
     6541 cggcgtcccc tttgccggat tcggtggtga cgggggcgcg gcgagcatgg ctgagttctg
     6601 cgcgcccgac ggtctggcct tcgacaagac aggaagcctc tacatcgctg actcgtgcaa
     6661 taaccgcgtt cgcaaagtca cctacccagc tccggcggcg accccaacat ttagcgttgc
     6721 atctggcacc tatgctgaag cgcagactgt aaccattaca gatgccacac aagacgctgc
     6781 tatctattac acgacggacg gatctgttcc aacaactggc tcaactctct ataatgcgcc
     6841 acttactgtt gcctcgtcgg agaccgtcaa ggccatcgcg gtggcagccg gctatacggt
     6901 gagcgccgtg gcctcggcga actacgtgat cggcaaccaa aaagctgtgc ctgacgtggg
     6961 tgtcgtgaag tcgtctctga atccatccat gacaggcacc gcagtcacat tttcggtcac
     7021 cataagctcg ggtctcggtt caccgtcggg gacggtcaca ttcatggatg gagctgcgca
     7081 gttgggctcg ggcacgctga caggcggctc cgcgaactac accacgtcgg cacttgcggc
     7141 aggctcacat tccatcacgg cagtctactc aggggacgtt aacttcacgt cggtgacgag
     7201 taccgcactc acccaggtgg tggagagctt ctcaattgca ctgccaggtg gctcatcgtc
     7261 caccgcaacc gcctcgcctg gcgggcaagc caaatacacc ttcgtcatta ctcctccggc
     7321 gacggggagt gcgctcacgt tcgctgtgac tgggctgcct gcgggggcaa cgggaacgtt
     7381 ctcacccgcg acggtagctg cgggcgcagg agtgacaaat gtaaccatga ttgttaccct
     7441 tcctgccacc atagcaatgc agtccgatga gcagccgttt gggcgcggcg cgttggcttt
     7501 gtcatttggc ctggtgcttc tgccgtttgc acgcaggatt ggcaggggat ggcggaactt
     7561 gccatcgctg cttctcctga ttggcattgg cgtgacgttc tgctttggag taagcgcatg
     7621 cggagggagc ggcccaaagc agtcgccttc tccgcagaca tacacgctga ccgtaacggc
     7681 gtcttccggg acactggcgc agtcgaccac tgtgaccctg attgtccagt aatggtggag
     7741 cgctcgaatt taaacggatc gctcgctcat tgtccggtcg atgggatggt tttttgcccc
     7801 atgctcgtta agcgctctga ggggaagatt gttccctggt ccacaccttg tggaacggcc
     7861 ggatcttatc gatccggcca ttgatttggt gggatttagg agggtcgttt ttcgaggcct
     7921 tttgaaacgg cccggagcat cttgtgaagg tcatggagga tgccgcggat ctcccggatc
     7981 tgg
//
LOCUS       DASZYN010000464         2845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_24100, whole genome shotgun sequence.
ACCESSION   DASZYN010000464 DASZYN010000000
VERSION     DASZYN010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24100"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="VHE33_11920"
     CDS             complement(<1..1139)
                     /locus_tag="VHE33_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98205.1"
                     /translation="MATHAPTILTPEERLARRRLIVQDAVSLLTLFLITAVLFALTYL
                     LFRSFNNHREELGARWKARGEAALNGGHPAVAIDDLRSALAYIPSRETEVELATALAQ
                     AGKTQEATAYFTTLRESAPGDGTINLQLARLAARGNNQAQTVLYYQSALDGTWQGNGY
                     DRRRQVRLEMARYLIARREFNQARNQLLIAAGNAPDNPAIKIEIAQLLEQAQAPRDAL
                     NQYQILAARRDPPFASLEGAGRTAFALGMYRMAAEYLGRALAGALPANFPTNQNSADH
                     FMLDTSQRVLLLNPSLRLSPRQRAQRVLALRNIARARFTACNSSAPSQLGDLAARWNQ
                     IPSALTVAKLEQQPDLEQNIIQLAYDTETATAAVCGAPEGDDATVL"
     gene            1249..2100
                     /gene="larE"
                     /locus_tag="VHE33_11925"
     CDS             1249..2100
                     /gene="larE"
                     /locus_tag="VHE33_11925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HVT98206.1"
                     /translation="MNLEEKEKTLNQRLRDRGSVIVAYSGGVDSAYLAWAAWRVLGSK
                     MLAAIADSASLARSHYRDAVAFAEENAIPLLVLETAELEREAYAANNGDRCFHCKDEL
                     FTVLEAERVRRGFAAVAYGLNLDDGKDFRPGQRAAANHHVAAPLAEAGLSKADVRELA
                     RRAGLRVWDKPASACLSSRIEYGRRVTTEALRQVEDAEEGLRALGLRRFRVRHHGSVA
                     RVEIAEEEMAGALDPEFFRRMAGVVRSAGFPYVAVDCDGYRSGSMNELLPAEMLGSTG
                     RSNRGAS"
     gene            2122..>2845
                     /locus_tag="VHE33_11930"
     CDS             2122..>2845
                     /locus_tag="VHE33_11930"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HVT98207.1"
                     /translation="MIECFAGISGDMLLGALIDAGVPAETLQEAARALGIGAQLRVST
                     VSRSGVQATKVDVLENGELAESAGHGVAREGTHEHHHHAHEHDSGHHPSHSHVHGRHW
                     PEIRQLITGADLSPGARAIALRAFELLAEAEGKMHGIPAEQVHFHEVGAVDAIVDIVC
                     GAVGLDSLGMEKWVCSAVNVGSGFVNCAHGRMPVPAPATAELLRGVPTYSDGPQMELT
                     TPTGAAMLRALDCSFERDGAVAN"
BASE COUNT          513 a          761 c         1041 g          530 t
ORIGIN      
        1 agcaccgtcg cgtcgtctcc ttcgggcgcg ccgcacaccg ccgcggtggc cgtttcggtg
       61 tcataagcga gctgaatgat gttctgctcg aggtcgggtt gctgttcgag cttcgcgaca
      121 gtcagcgcgc tggggatctg attccatcgg gcagccagat cacccagttg cgaaggcgct
      181 gagctgttgc aggcggtgaa ccgtgcccgg gcaatattcc tgagcgcgag aacacgttgt
      241 gcccgctgac gcggagacag gcgaagagaa ggattcagga gcagcacgcg ttgcgaagta
      301 tccagcatga aatggtcggc tgagttctgg ttcgtgggga aattcgcagg aagagcgccg
      361 gccagggccc ggccgaggta ttcggccgcc atccggtaca tgccgagcgc aaaggcagtc
      421 cgccccgcac cttcgaggga cgcgaaaggc ggatcgcggc gtgccgccag aatctggtat
      481 tggttcagag cgtcgcgcgg agcctgcgcc tgttcgagca actgggcaat ttcaatcttg
      541 atggcgggat tgtcgggcgc atttcccgcg gcgatgagaa gctggttccg cgcctgattg
      601 aattcacgcc gggcgatcag gtaacgcgcc atctccagcc gaacctggcg acggcgatcg
      661 tagccgttgc cctgccaggt gccatcgaga gccgattggt aatagaggac ggtctgagcc
      721 tgattgttgc ccctcgcagc tagacgagcc agttggagat tgatcgtccc gtctccaggc
      781 gccgattcgc gaagagtggt gaaataggcc gttgcctcct gggtctttcc tgcctgggcc
      841 agcgctgtgg ccagctcgac ttcggtctcg cggctcggaa tgtaggcaag cgcggatcga
      901 agatcgtcga tggccacagc cggatgacca ccgttcaggg cggcctcgcc ccgtgccttc
      961 caccgggcgc caagctcttc acgatggttg ttaaaggatc gaaagaggag gtaggtgagc
     1021 gcgaacagaa cggccgtgat cagaaacaga gtcagaaggg aaacagcatc ctgcacgatg
     1081 agccgtcgac gggcgagccg ttcttctggc gtgaggatgg ttggcgcgtg ggtggccatc
     1141 tgctctagtt tctcagattt gcaggctcat tctgggcggg cgcagtggga cccggcgatt
     1201 tccaacgtgc gagtttgcgg gcgtaggatg aggaacagag gcctggggat gaacctcgaa
     1261 gagaaggaga agacgctcaa ccagcggctg cgcgatcggg gcagcgtgat cgtggcgtat
     1321 tccggcgggg tggactcggc ctatctggcg tgggcggcct ggcgggtcct gggttcgaag
     1381 atgctggcgg cgattgccga ctcggcgtcc ctggcgagga gtcattaccg cgacgcggtt
     1441 gcgtttgccg aggagaatgc gattcccctg ctcgttctgg agacggctga actggagcgc
     1501 gaggcctacg cagcgaacaa cggcgaccgc tgctttcatt gcaaggatga actgttcacg
     1561 gtgctggagg ccgagcgagt ccggcgaggg ttcgctgcgg tggcgtacgg gctcaatctg
     1621 gacgacggca aggattttcg accggggcag cgagctgctg ccaaccatca tgtcgcggct
     1681 cctctggcgg aggccggact gagcaaggcc gacgttcggg aactggcgcg ccgtgcgggg
     1741 ttgcgggttt gggataagcc ggcatcggcg tgtttgtctt cgcggattga atatgggcgg
     1801 cgcgtgacga cagaagcgct gcgacaggtc gaggatgcgg aagagggatt gcgagcgctg
     1861 ggactgcggc ggttccgggt gcggcatcac ggcagcgtgg cgcgcgtcga gatcgcggaa
     1921 gaggagatgg ctggcgctct cgatccggaa ttcttccgca ggatggcggg agtggtgcgg
     1981 agcgctggct ttccttatgt tgcggtcgac tgcgacggct accgctccgg ctcgatgaac
     2041 gagttgctgc cggcagagat gctgggaagt acaggacgga gtaaccgagg agcgtcgtaa
     2101 cccatactca tgaagatcgg aatgatcgaa tgttttgccg ggatctccgg ggacatgctg
     2161 ctgggtgcgc tcatcgatgc cggcgttccg gcggagaccc tgcaggaagc cgcccgggcg
     2221 ctggggatcg gcgcgcagtt gcgtgtttcc acggtgagtc gcagcggagt gcaggcgacg
     2281 aaggtggacg tgctcgaaaa cggggaactg gcggaaagcg cggggcacgg agtggccaga
     2341 gagggcacgc acgagcatca tcatcacgct catgaacacg attcagggca tcacccgtca
     2401 cattcgcatg tacacggccg ccactggccg gagattcggc aactgattac gggtgcggat
     2461 ctctctccgg gagccagggc gattgcgctg cgggcgttcg agctgctggc cgaggcggag
     2521 ggaaagatgc atggcatccc cgctgaacag gtgcatttcc atgaggtcgg agccgtggat
     2581 gcgatcgtcg acattgtgtg cggggcggtc gggctggatt cgctgggcat ggagaagtgg
     2641 gtttgctcgg ccgtgaatgt cggcagcggg tttgtgaact gtgctcatgg gcggatgcct
     2701 gttccggccc ccgcgacagc ggagcttttg cggggggtgc cgacatactc ggatggaccg
     2761 cagatggaac tcacgacccc gacgggcgcg gcgatgctgc gggcgcttga ttgcagtttt
     2821 gaacgggacg gagccgtcgc aaaca
//
LOCUS       DASZYN010000465         4930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_400382, whole genome shotgun sequence.
ACCESSION   DASZYN010000465 DASZYN010000000
VERSION     DASZYN010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400382"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /locus_tag="VHE33_11935"
     CDS             complement(<1..1571)
                     /locus_tag="VHE33_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HVT98208.1"
                     /translation="MTLRQKMLLMLALTVMGVVAAIGWAGSVRIRHVFEAMDRQQTDL
                     LVGQFQHEFNQRASETAAVMDRMAASDVMKRIAFELNNGGDAATYLTVALPLAQEYRL
                     EYLELVAHDGSIISSLQWTARFGYKEPAIATAGKPAFLKQEDLPDGTSQIGLFAARTV
                     AGSDPPLYLVGGRQLDASFLKDFSVPAGTQLLIYRNVSGAFETRNFAGPVEDLKSAGS
                     YQALVDDARTNGRNAEGVVYPTRHREDSMTVTAIPLKASDGTVLAVLVIANSRRGMIE
                     VQAHIRDIAYGIAGLGIIFAVVASLWIAARISRPIEELARAAGRVAAGDWEARVDIRS
                     LDEVGDLARSFNNMTRQLSEQREKLLQTERVAAWRELARRLAHELKNPLMPLQITVEN
                     MVRAKKLPKKQFDEVFKESTATLTEEIDNLKTIVGRFSDFSKLPKPEAVEMDAREAVR
                     RVVKLYGPALEEKHIELGTSITAEPLSILGDSELLHRALSNLVLNAMDAMPEGGTLTV
                     SAVRAKETARISVSDT"
     gene            complement(1568..2917)
                     /locus_tag="VHE33_11940"
     CDS             complement(1568..2917)
                     /locus_tag="VHE33_11940"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVT98209.1"
                     /translation="MARRFVISVVLICLTLSATARTRPHYGGTLRIETQGDPWQIPDG
                     IARRLVLDTLTTVSDTGGAQAALALRWESQNAAHRWEFWIRPGVHFQDGEPLTSDAVA
                     GALTDICSRGAAGACPWRSVRGVGASVVFVSDSPEPDLPELLAQTQFAIGRQDAKGAL
                     TGTGRFRVTGYTNGALTLVANDDCWSGRPFADMVEVRTRRTVRDQWLDLSVGRADIVE
                     VPPELLRQAQQQRMNVLVSRPVDLLALTITRSVPFASREMRQAAALAVDRMALYNVIF
                     QKQGEVTASLLPEGLSGFAFLFPSDRDLDRSRALRGGATVPPMQMGVSDTSATMLLVA
                     ERLALNLHEAGFNVQVSTAGSRPTGALELRKVHLEATDPGAALEEILARFGENGSVTG
                     ADASLLWQEERGILDQSAIVPLLWLPRAWAAGDRVRDLRLLSDGEPDLSDASLEGGK"
     gene            complement(2895..4169)
                     /locus_tag="VHE33_11945"
     CDS             complement(2895..4169)
                     /locus_tag="VHE33_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98210.1"
                     /translation="MMKTCWAVVVAATLACLTPAAHAATWDQPVAELAKQIAGLSGPG
                     PAKLILQNASGLSALDVPVIRRLMEHDLRGFGVIAGDRDSATLIRITLSQNLQGGLWV
                     AEVVEGTATRVTMLRVNLGGATSASGPSSLVVQRTLLLTVPEPMLDAQVFTVGTLARL
                     VVLEPEQIVTWVRSSAGLAFGGAPAGSGWTEDQHFPIAHTRPYPRDLRGELVPAQDHL
                     FDAYLPGVQCAGTNTGAQVAVTCGDSDDPWPVSGPVGNQVAGTVGGPAGVPQRAFYNA
                     MRDFFTGVLAPGFGMDLPPFYQASDIPRPTGTGLLLNTVDGRLMLIENNMLKPLTGAA
                     DWGSDFAVLRSECGSGVQVLVSGSGSAAAGDSLRAYELVGREVLPVSPPLPVEGSVTA
                     IHANHNGTSATVVVRRDASQRYEVWNGSALCH"
     gene            complement(4373..>4930)
                     /locus_tag="VHE33_11950"
     CDS             complement(4373..>4930)
                     /locus_tag="VHE33_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98211.1"
                     /translation="QTDIQQKQVRIAELQAEAQAAQKVKQAEGDAHARVVEAKGEADA
                     MQYTLPLKQKQIQQSKLEAEARKEATIQNAEAEAQAKVIDSKAEVQRRNLLADAEANR
                     IRVTAVADADRMASEAKVLNQSPLLINKIIAERLSDKIQMVMVPSDGKYFFASDVFKG
                     LAQQSDTPSGQNAGRDSASLPGGQN"
BASE COUNT          906 a         1719 c         1375 g          930 t
ORIGIN      
        1 gtgtcggaca cggaaatcct cgccgtttcc ttcgctcgga cggcggacac cgtcagcgtt
       61 ccaccttcag gcatggcgtc catcgcattc agcacgaggt tcgacaacgc gcgatgcagg
      121 agttctgaat cgcccaggat cgacagcggc tccgcagtaa tcgaggttcc cagttcaatg
      181 tgcttctcct caagcgccgg cccgtagagc tttaccaccc gccgaaccgc ttcgcgcgca
      241 tccatctcca cggcttcggg tttcggcaac ttcgagaaat cgctgaagcg tccgacaatt
      301 gtcttgagat tgtcgatctc ctcggtcagc gtggccgtgc tctctttgaa cacctcgtcg
      361 aactgcttct tcggcaactt cttcgcgcgc accatgttct caacggtgat ctgcagcggc
      421 atcagaggat tcttgagctc gtgtgccagt cgccgggcca gctcacgcca cgccgcaaca
      481 cgctccgttt gcagtagctt ctcacgctgt tccgagagct gccgtgtcat gttattgaaa
      541 ctgcgcgcca gatcgccaac ttcatccagg ctgcgaatat ccacgcgtgc ttcccagtcg
      601 cccgctgcca cgcgtcccgc cgcacgcgcc aactcctcga tcggacgcga aatgcgtgcg
      661 gcgatccaaa gactggccac gaccgcaaaa atgattccca gcccggcgat tccgtaggcg
      721 atatcgcgga tgtgggcctg cacctcaatc atgccgcgcc gcgaatttgc aatgaccagg
      781 accgccagca cggtgccgtc tgaagccttc agcgggattg cggtcacggt catgctgtct
      841 tcgcgatgcc ttgtcggata cacaaccccc tcggcattgc gcccgttggt tcgggcgtcg
      901 tccaccagcg cctggtagct tccggcgctt ttcaggtcct ccaccggacc ggcaaagttg
      961 cgcgtctcga aggcccccga cacgttgcga taaatcagca gctgcgttcc cgccgggacc
     1021 gagaagtcct tcaggaaact ggcgtccaac tgccggccgc ccaccagata caacggcgga
     1081 tcggaccccg caaccgtccg cgcagcaaac agtccaatct gcgaggtgcc gtccggcagg
     1141 tcttcctgct tcagaaaagc tggcttcccg gcggtcgcaa tcgccggctc cttgtagccg
     1201 aaccgtgccg tccattgcag cgacgagatg atgctgccgt cgtgcgccac cagctccaga
     1261 tactccagcc ggtactcctg cgccaaaggc agggccaccg tcagatatgt cgcagcgtcg
     1321 ccgccgttgt tcaactcaaa cgcgatgcgt ttcatcacgt cgctggccgc catgcgatcc
     1381 atgaccgcag cagtttcgga ggcccgctgg ttgaattcgt gctggaactg gcccaccagc
     1441 agatcggtct gctgtcggtc catcgcttca aaaacgtgcc ggatacggac tgagcccgcc
     1501 catcctattg ctgccacgac gcccatgacc gtcagagcga gcatcagcag catcttctgc
     1561 cgaagcgtca cttccctccc tccagcgacg cgtcagataa gtccggctcg ccgtcagaca
     1621 aaaggcgcag atcccgcacg cggtcgcctg cagcccacgc acggggcagc cacaacaacg
     1681 gcacaatcgc cgactgatcg agaatcccgc gttcttcctg ccaaagcaaa ctcgcgtctg
     1741 cacccgtcac gctaccattc tccccgaagc gggccagaat ctcttccaga gccgcgcccg
     1801 gatcggttgc ttcaagatgt acctttcgca gttccagggc tcctgtcgga cgcgaacctg
     1861 ctgtcgaaac ctggacattg aagcctgctt catggaggtt cagggccagg cgttcggcta
     1921 ccagcaacat cgtcgcactc gtgtccgaca cgcccatctg catcggcggc acggtcgcac
     1981 cgcctcggag cgcccttgag cggtcgaggt ccctgtccga ggggaacaaa aacgcgaagc
     2041 cgctcagacc ttccggcaga aggctggcag ttacctcgcc ctgtttctgg aagatgacgt
     2101 tgtacagggc catgcgatcc acggccagcg cggccgcctg gcgcatctcg cggctggcga
     2161 acggcacgga tcgggttatg gtcagtgcga gcagatctac cggtcgcgac accagcacat
     2221 tcatgcgctg ttgctgcgcc tgccgcagca gctcgggagg aacctccacg atatcggcgc
     2281 gtccgacgct caggtccagc cactgatccc gaaccgtgcg acgcgtccgc acctccacca
     2341 tgtcggcaaa cggtcgtccc gaccagcaat cgtcgttggc caccagcgtc agggcgccgt
     2401 tcgtgtatcc ggtcacgcgg aaccggcccg taccggtgag ggctcctttc gcatcctggc
     2461 gcccgatcgc gaactgcgtc tgcgccagca gctccggcag atccggctca ggagaatcgc
     2521 tgacaaacac aaccgacgct cccacgccgc gtacgcttcg ccacggacag gccccggctg
     2581 cgccgcggct gcagatatcc gtcaatgcgc cggcgactgc gtccgacgtc aggggttcac
     2641 cgtcctggaa gtgcacgccg gggcgaatcc agaactccca ccggtgtgct gcgttctgcg
     2701 actcccagcg cagagccaat gccgcctggg cgccgccggt gtcagaaacg gttgtcagcg
     2761 tatccagcac cagccggcgt gcgattccat ccgggatctg ccagggatcg ccctgagttt
     2821 cgatgcgcag ggttccaccg taatgcgggc gcgtccgcgc cgtggcagac aatgtcagac
     2881 aaatcaaaac cacgctaatg acaaagcgcc gagccattcc acacctcata tcgctgcgac
     2941 gcatccctgc gcaccacgac ggtcgcgctg gtcccgttat ggttggcgtg gatggccgtc
     3001 accgagccct ccacgggcaa aggtggactc accggcagca cctcacgtcc gaccagttca
     3061 tatgcgcgca ggctgtcgcc ggcagcggca gagcccgaac ccgagaccag cacctgaacc
     3121 cccgatccgc actcggaacg aagaactgca aagtcgctcc cccagtcggc tgcgccggtc
     3181 aggggcttga gcatgttgtt ctcgatcagc atcaaccgac catccaccgt attcagcaat
     3241 agcccggttc ccgtcggacg cggaatgtct gacgcctgat agaacggcgg aagatccatc
     3301 ccaaaccccg gcgccaaaac gcccgtaaag aaatcgcgca tggcgttgta gaacgcccgc
     3361 tgcggcactc cggccggacc gcccaccgtt ccagccacct gatttccgac cggcccggac
     3421 accggccatg gatcatcgct gtcgccgcac gtcactgcaa cctgcgcccc cgtgtttgtg
     3481 ccggcacact gcacgcctgg caaatacgcg tcgaacaaat gatcctgcgc cggcaccagc
     3541 tcgccgcgaa gatcgcgcgg atacggccgc gtgtgcgcaa ttgggaagtg ctgatcctcg
     3601 gtccagccgc tgccggcagg cgcgccgcca aaagcgagcc ccgcgctgct cctcacccag
     3661 gtcacgatct gctccggctc cagcaccacc agccgcgcca gcgtcccgac cgtgaagacc
     3721 tgcgcatcca gcatcggttc tggcacggtg agcaacagcg ttcgctgcac gacgaggctc
     3781 gagggtccgg atgcagacgt tgccccgccc aaattcacgc gcaacattgt tactcgcgtt
     3841 gcggtgccct caacaacttc agcgacccac aggccgccct gcaaattctg cgagagcgtg
     3901 atccggatca gcgtcgcgct gtcccggtct cctgcgatca cgccgaaccc gcgtaaatca
     3961 tgttccatca accgccggat cacaggaaca tcgagcgccg acaaacccga agcattctgc
     4021 aggatcagct ttgccggtcc tggtccgctg agccccgcga tctgcttcgc gagctcagct
     4081 acgggctggt cccaggtggc tgcgtgagcg gccggcgtca gacacgcaag cgtggcggct
     4141 acaacgacag cccaacacgt cttcatcatg attcgctggg gcggattata tcgtgcgctt
     4201 tgcattgccc tgctcccaca aaggttccac ctcggtcgcc gtgtccgaca accgaggtgg
     4261 catcccgctc tctcgagagc ccgtctccga cgagccgagt ttgcaggaac tggccgttct
     4321 ggcagagcgc tttgcggcgg atgccagatg cccacaagca gcatcgcgtt cgtcaattct
     4381 gccctccggg caagctggcc gaatctcgtc ccgcgttttg ccccgaaggc gtgtccgact
     4441 gttgggccag tcctttgaaa acatcgctgg caaagaaata ttttccgtcc gaaggcacca
     4501 tcaccatttg aattttgtcc gacaggcgtt ccgcgatgat cttgttaatc agcagcggcg
     4561 attggttgag gaccttcgcc tcgcttgcca tgcgatccgc gtccgccacg gctgtcaccc
     4621 gaatccggtt agcctcggca tccgccaaca gattgcgccg ctggacttcg gctttgctgt
     4681 cgatcacctt ggcctgtgcc tcggcttcgg cattctgaat cgttgcctcc ttccgcgcct
     4741 cggcctcgag tttcgactgc tggatctgtt tctgtttcag gggcagggta tattgcatcg
     4801 catccgcctc gcctttagcc tcgaccactc tggcatgggc gtcgccttcg gcctgtttca
     4861 ctttctgcgc agcctgcgcc tcagcctgca gttcagcaat ccgaacctgc ttttgctgga
     4921 tgtcggtctg
//
LOCUS       DASZYN010000466        37885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_789063, whole genome shotgun sequence.
ACCESSION   DASZYN010000466 DASZYN010000000
VERSION     DASZYN010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 37885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37885
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789063"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..553)
                     /locus_tag="VHE33_11955"
                     /pseudo
     CDS             complement(258..553)
                     /locus_tag="VHE33_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558256.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(790..2103)
                     /locus_tag="VHE33_11960"
     CDS             complement(790..2103)
                     /locus_tag="VHE33_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98212.1"
                     /translation="MKYTIRNIIRSRKLLTGPAFAALLAGFAVGPSVFDKGDVAEAQD
                     IVPGSTMVPKFEVDPSWPKPLPNHWMLGETIGISMDAHENIWVLHRPPSLEKNASSLT
                     RGEAKCCTAAPDVLEFDEAGNLIHYFGPPEGHDWPAGNHGITIDKDGNVWMAGASAAQ
                     PGPPPGSAEQFAKHPNGGAFDEETGGEFVKGQYHDSFLLKLSQDGKFLGEIGHANGSK
                     GSLDTDNVRGVATIRFLPTGELVAADGYGNHRVSVWDPVTMKLIRMWGAYGKPPTDDP
                     IPHYNVNSPQFGNPVHCAQPSNDGLLYVCDRTNDRIQVFKFDGTFVKQYGVAVNTRAL
                     GSVWEIAFSKDPAQKFMYITDGSNEEIHVFDRQSMKELYSFGGGGRQPGQFYGAHSIV
                     TDSKGNIFTTETFDGARVQKFIYKGMVPLSSLLKEHVVGRSIINP"
     gene            2574..2984
                     /locus_tag="VHE33_11965"
     CDS             2574..2984
                     /locus_tag="VHE33_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98213.1"
                     /translation="MKKFLIASCAVLLLPTAAFADHDAEMIIAYRYSDLAFRAPTIDL
                     VHMHLHQVVNCLVGPGGPGFDATYTNPCAEAGNGAIPDAPESSMEFQMRIVLATVNAG
                     LAATDIATAKKYAGDASATINSGQCALATGVHYG"
     gene            3097..3537
                     /locus_tag="VHE33_11970"
     CDS             3097..3537
                     /locus_tag="VHE33_11970"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HVT98214.1"
                     /translation="MRISIAMFFLFFVSLIPTVAATPNIRVDGAWAAPTPNGAQTGVA
                     YMVIHNQGPNDDRLVGGTSPAADKIQVHEMKVTNDIMEMREVVDGLPVAAGRTAELKP
                     GGFHMMLIGLKRQLRAGQDIVLTLRFEHAGEVLVHVPVRADSRR"
     gene            3585..4178
                     /locus_tag="VHE33_11975"
     CDS             3585..4178
                     /locus_tag="VHE33_11975"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HVT98215.1"
                     /translation="MRLQRYILMAGVIVVAATTSSAATGLPTSHISNKPTPIPGIGGP
                     FNLIDDNGRKVSAATLAGKPSAIFFGYTFCPEVCPTTLQDVTSWISALGSDAGKINYV
                     FVTIDPHRDTPKVMHQYLSSFDRRIRGFSGTDEQTAKIAREYGAYYKRVPAAGGSYVM
                     NHSDRIYLMNAQGQYVGSISYREPDASAISKLKQLLQ"
     gene            complement(4340..5422)
                     /locus_tag="VHE33_11980"
     CDS             complement(4340..5422)
                     /locus_tag="VHE33_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98216.1"
                     /translation="MKFAAPATLLAATAMLLTTTAMALAQGADATPENNGPLAPYHIQ
                     HGWLQLPMGRKWGAASAVDIDRDGKSVWVFERCGNTDDGCDLHKDVNPILKFDANGVL
                     VKSFGAGMFHDPHGIYVDKDDNIWVVDSINYSGGIGDTIRKFSPDGKLLLTMGTDGVA
                     GYGPYVYNGLSDVVLAPNGDIFVADGHGSPTNNNRIIKYDSKGKYIKEFAPRGSAPED
                     TNPPHGLAMDKEGRLYVADRNNHAVKIFDQDGKLLNVWKQFGSPSGLFIDKNDILYVS
                     DFNSTVKSNPGSGITTPGIRWGSVSDGKVTGYIPYAEFASLEAVAVDDDGIIYGGLTN
                     IPGSVRWLKNPPAAPRPAAPAAAPAP"
     gene            5845..6396
                     /locus_tag="VHE33_11985"
     CDS             5845..6396
                     /locus_tag="VHE33_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVT98217.1"
                     /translation="MHIIAKLFLGTALLAGAVTGALAQAQPGDPPIPTYNDPPGPLPN
                     PLNITMHPGSVFPENRVFGDPSKPGIYGMITTWQPGKFSRPHFHNTDRFVYVVSGTWW
                     VSASNDFDPKTTYPIPQGTFAVDLANKVHWDGNRLGGPPTVLYIVGEGPLTNISVGHD
                     GKPLAVQPNHQQRPAAPAAAPAP"
     gene            complement(7595..9616)
                     /locus_tag="VHE33_11990"
     CDS             complement(7595..9616)
                     /locus_tag="VHE33_11990"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98218.1"
                     /translation="MKSLSRRTLLLTAIGGYLASWLPGWAQARAPDTEWRNYAGDPAN
                     TRYAPLDQIHAGNFNDLEIAWRFKAGSLGPYPEFLEQCTPLVVKGRLYATAGSRRAVV
                     CLNAATGEILWKHSEDEGARADASPRKYSGRGVAYWTDGKDERILYVTTGYRLIALDA
                     HTGAPVRSFGVDGIVDLKENDDQIIDLVNGQVGLHATPIVAKNVVIVGAAHPAGDVPK
                     RSFGVKGYVRGFDVRTGERKWIFHTIPRKGEFGYDTWIKAGDAERADNTGCWGQISVD
                     LELNTAYLPIELPTGDQVGIYRQGPALFGESIVAVDIDTGRRKWHYQLIHHGLWDRDI
                     PCAPILCDIPYEGKIVKALAQPTKQAFLYVLNRETGEPVWPIPETPVPAGDVPGEWYS
                     PTQPIPSRPPAYGVQEVNSDTIIDFTPELHAKGLEFISHFRTGGVFNPPTLATVGGNW
                     GSLCAPSLLGGTNWPGGCYDPETHKVYVFAQNSVAILGIVPGDPKETDFQYVHGTPSA
                     QRLTGQNTTGGGAGPTPVTPDGLTTIDGLPLLKPPYGTLSAIDLSNGDITWQVAHGDT
                     PDNIKNHPALKGLKIPRTGRTGILGPTVTKTLLICGEAGFGISPQGVRGAMLRAYDKA
                     TGAERGAVYMPGPQTGAPMTYMLDGRQYIVLGIGGDHSAELIAFRLPRI"
     gene            complement(9721..10698)
                     /locus_tag="VHE33_11995"
     CDS             complement(9721..10698)
                     /locus_tag="VHE33_11995"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HVT98219.1"
                     /translation="METTRRFFVGMGAATAAMGLDQALAAPAHASMAKDKFWVAAVTP
                     CDKSYNFDEGAYRDLLSWYKANGADGVLVLGTNGEGPSYSVAERKKIAEFALKNKMGL
                     DMIVATGTANFNETIELSQHAAAIGADSVLIQPPFYFKDPAGDGVIRYFTAIFDKVRT
                     PVRYYHIPQVTGVPVGLKVFQALAQHPNFIGIKDSHGDPAEYEVIANGLPARLNIISG
                     TDNNMPAALRRGDGVILGSGNVYPKYVAAIFAAHRAGKDLTAPLAKMARATSFMEGGD
                     YYNPSAIKYTLSLMMGNLGTTFTRPPWTEIGEDLKAKIRKGVSQLKAMG"
     gene            complement(10756..11874)
                     /locus_tag="VHE33_12000"
     CDS             complement(10756..11874)
                     /locus_tag="VHE33_12000"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HVT98220.1"
                     /translation="MVTSFNDPVARRQFLSGGLAVFGVTAAAGMTDASPAWGAMASLD
                     PVNPGIRFGLAGGAFGRWPKRGAPMTDSQDIPGMIKATARYGFQGFEPYSTQIGDYLN
                     KPLAIKRLCDDAGIQFIDIGDMPRSLGPQPPPTPPGVVKDTNPFGSGPYPWLGEEGNA
                     ELITAMVQFARTNLAPAGCDHWKMNMGGRPKGGPSPAQLKALARTLNEIGRQTIAFGV
                     RAAPHPHIWGPMEREHEFRTVMELTDPRYVWLTLDTGHNVLGGMDTVKIVNDYLPRIA
                     EFHLKDTYAKYRGNKVTLANSEYLKGNIWGTMGEFGGVDFPGIFDVLRKRKWKGWAVV
                     DVDGPRPGEGTVEDTLAKNVNYLRDVLHIKMPSPPHSP"
     gene            complement(12053..13042)
                     /locus_tag="VHE33_12005"
     CDS             complement(12053..13042)
                     /locus_tag="VHE33_12005"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVT98221.1"
                     /translation="MATQQTQTWGVRMLEIQNSAVDSNLKAGENLQEWNTIVGEAFSG
                     CVVDTSKSHFSGELWRCKINDMNLVRVRAGASRAERWITGAPRYSSGSVLLHLQSVGR
                     SISGQRGRCAAVDPGEAAICDPDYNYSVDFLTPYEMFVVELPIAGILAREPGFDLERS
                     AAKKVDVKRSQLLLAFLRTAWQQRECLSNDADWRDCVSRTSLDLAMRAISRAEGNEVV
                     GASAELRRAVIEHIRHHLADTELRTSSIARALKISSRSVQTVFERLATTTSGFILEQR
                     LARAGECLVREPGGKSITELAYDCGFSDSAYFSRCFKRHFGVTPRDYRRDGPT"
     gene            13137..13475
                     /locus_tag="VHE33_12010"
     CDS             13137..13475
                     /locus_tag="VHE33_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HVT98222.1"
                     /translation="MPAANFRHTGGISARNRQDHRYQAARLIHARSRRAGNAFVAINC
                     ATMEPNRPEAELFGVESMEGPRKTAASEYGMDVAFVKKVIAQSAVYRGVPAANAALHM
                     VVRLTVQNPG"
     gene            complement(13472..14362)
                     /locus_tag="VHE33_12015"
     CDS             complement(13472..14362)
                     /locus_tag="VHE33_12015"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HVT98223.1"
                     /translation="MASTKLMITVAPTGGMASKAQNANLPTQPGEIAESVHKSFKAGA
                     CIAALHARRPDDQATCNADIYRDINSRIRERCDIILNNSTGGGSSGDMLFERPDGLFE
                     NRFEERLKGCEAGAEMATFDGMTFVDVHGGKEILVITPPSRCETLIKRITERGIKPEW
                     EVFAPNHILQDVTRLIQKGYDKPPYYINMVLGAHRGFQGAMPYSHDVLNVMIAALPPH
                     SIFCVSAIGPAQLPATTQAMLLGGHVRVGLEDNNYYSKGMLATNEQLVARAARIATDM
                     GLEIANPGEAREILGLTKLG"
     gene            complement(14349..14804)
                     /locus_tag="VHE33_12020"
     CDS             complement(14349..14804)
                     /locus_tag="VHE33_12020"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HVT98224.1"
                     /translation="MFVNRRTVTIDWGDCDPAGIVFYPCYFAMFDVSTAALFGAALSI
                     TKREILKRYRIIGFPMVDVRAKFYVPSSFGDIVTIESRVRRFGGSSVEIDHRLMRGDV
                     LAAECQEKRVWAEQNPDEPDSIRGVSVPQEVRESLLQQSANAGENHGLY"
     gene            complement(14807..15655)
                     /locus_tag="VHE33_12025"
     CDS             complement(14807..15655)
                     /locus_tag="VHE33_12025"
                     /inference="COORDINATES: protein motif:HMM:NF024740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HVT98225.1"
                     /translation="MSKRRSNTVKGFILDMDGTLAVGKPEGGGYLLLPGVADFIKELR
                     SHGLPFVVFTNGTAQTPERYAENLGAAGLELSPSEMMTPSSVASEYFAASGCRTVLVL
                     GGEGVASPLTQVGIAIVRPEHLLPHKVDAVYIGWHPSFGLADIEAAVAAVSAGAPMYV
                     SSDVPFFYSSKGRTLGISGMIGAAIAKAVGRRAVVIGKPSAIAAQMAARRLGCAESQM
                     AIVGDDPKLEMAMARLCGAIGIGVISGLTNRSAWDALAPKQMAHHVVDRIDEIITRKL
                     VRLEGR"
     gene            complement(15648..17123)
                     /locus_tag="VHE33_12030"
     CDS             complement(15648..17123)
                     /locus_tag="VHE33_12030"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98226.1"
                     /translation="MAEIGLVDVSMRDGNQSLWGATGLRTSQMLEIAGQLDRVGFRAI
                     DFTSSSHMAVAVRYFQDNPWERIRRMRAAMPRTPLQFITTGLRFIAWEQADPDFMRIV
                     YRRLQANGIRRFILLDPMHDVQAVLQTARIIKEEGDATTMAALTYTLSDMHDDAFYAR
                     FAALLAASADIDLFYLKDPSGLMSPERARTLVPAVKAVIGGKRLEVHLHCTIGLGGLA
                     SLAAVEAGVDAVHVGIGPLGNGTSLPEAGRMIANLLELGHTVAVDQGALEAVSRYWNR
                     LAVAENLPRGMPQEFDASFLRHQIAGGVMTTMLRQLAEVKLEGKLPEVIAETVRVRAD
                     LGYPIMVTPFPQMVCSQALFNVISGKRYSQVSDQVIRYVMGRFGRPTRPVASEVEAAI
                     LDRPRARELSAEPAVLGLQDMRRRFRADMPEEEFLLRAVMPGDQVDAMLKAGPDSARY
                     LPEAAPFHSLLRELAARPAARDIVVERPGFRLSLHAGPSLV"
     gene            complement(17116..18465)
                     /locus_tag="VHE33_12035"
     CDS             complement(17116..18465)
                     /locus_tag="VHE33_12035"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HVT98227.1"
                     /translation="MAFEMIRTLFIANRGEISLRIIRTAKRMGIATVLGVSEADRNAL
                     PALMADGIVVLGPAPSGQSYLDVDRVVGAAVKSGADAVHPGYGFLSENARFARAVRAE
                     GMIFVGPSPESLDAVGDKLKARAVGAAAGLPIVPGGQAADLGEARKHASEIGYPLLIK
                     AVSGGGGRGMKRVDREADLEAMVAMAMAEAQAAFGDPRVYIERYITRGRHVEVQVLGD
                     GRRAIQLGTRDCSIQRRFQKLVEEAPAPDIPPAQHAALCLAAVRLAESLAYQGAGTVE
                     FLVDAESFEFYFLEMNARIQVEHPVTEAITGVDLVEQQLLIADGQPMGIRQEDVTLNG
                     HAIEVRINAEDWTRNFLPSPGRITTARWPAGPHIRVDSHIESGGVVPPYYDSLIGKLI
                     VSGVDRTQAIARMSQALDTLILEGVATTAPMHRRIMADARFRAGAVDTRFFEGLADG"
     gene            complement(18455..18931)
                     /locus_tag="VHE33_12040"
     CDS             complement(18455..18931)
                     /locus_tag="VHE33_12040"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HVT98228.1"
                     /translation="MSDEIDLPLTPDDVASIVSLLNGSGYQGLELSTPRFVLKVARAE
                     GGGWTQEWQHPIPPERPIAGPPGEPAAAIEVEGLLAIRPPLPGTFYHTPQPGAPPFVK
                     MGDIVSPETIIGIIETMKVMNSIPAGFSGEIVEITAGHGDMVDQSAILMRLRPLGI"
     gene            complement(18928..20166)
                     /locus_tag="VHE33_12045"
     CDS             complement(18928..20166)
                     /locus_tag="VHE33_12045"
                     /inference="COORDINATES: protein motif:HMM:NF002674.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HVT98229.1"
                     /translation="MSKSKPRVLLLATLDTKAEEAAFLRGHLEGQGVEVVHLDASIRR
                     TVGNAEIPPSAVAAASGSSLELIRALKHEGKCQAEMTKGAIVAAHAADAAAPLAGIFA
                     IGGSMGTTLATAVMRSFSYGLPKVMISTLASGFTAPFVGTRDITMVNAVCDISGLNTI
                     SRDVYRNAALAVAGMAHGFRAQPDAGSRPLVLIGTLGTTERCSNRIRTELEARGYEVM
                     VFHTIGAGGQTMDQIVRERPVAAVVDMSLVEINDFLNGGLCSAGPDRSKAALEMGVPV
                     IFAPGNADFLIAGPIEDARARFPGKRYHLHNAALTAVRTEAAELNKLADHLAGLIENA
                     KGPVSMYVPLKGFSNHDSPEGYLHDPSLPPVFADYLTRALPKKVELYTLDRHINDAEF
                     ADALIGQVLAYTRNPQVAKE"
     gene            20431..21579
                     /locus_tag="VHE33_12050"
     CDS             20431..21579
                     /locus_tag="VHE33_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98230.1"
                     /translation="MSGLRVKSRRLVLLAVALALPAQAQTHDAPHYKVDPFWPGQLPN
                     KWIMGQVGGIAVDSQDHIWVLQRPRSATPDELGAAQNPPRSDCCMATPAVLEFDTAGN
                     VINSWGGPNFVKDWPTTEHAIWVDKGGDVWISGAGVDDRQVLKFTPDGHQILEIGRKS
                     KEPKNNQDTTMLGRAAAIEVDDAAHEVYIADGYLNNRIMVFDSDTGKFKRGWGGYGMP
                     LSQVSNSVEQIEGEVPAAGQIPPYAASDPPDGQFRSPVHCVRLSNDGLVYVCDRHNDR
                     IQVFTKAGKFVREFFLHPETLGNGSTWTMTFSHDVGQKYMLVVDGENNTIWIATRSDG
                     RVISHLGHSGRNSGQFHWVHQAAMDSMGNLYTGEVDTGKRVQKFVLDR"
     gene            complement(21809..22321)
                     /locus_tag="VHE33_12055"
     CDS             complement(21809..22321)
                     /locus_tag="VHE33_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98231.1"
                     /translation="MKIAHALFAVILPVMAVPVMAQPAAAQPAPPEGLFRSGADIPAL
                     IAKAKANQESPTINSVVPIVSVAPYLVLLEYRTGVTPPTVHHGQAELVHVLKGSADLA
                     TGGHLTGILPPTSGPGSASDVGTGIVGATKRHIVEGDYAMVPPDTAHQFQGVKGEFVI
                     MSVHLLMPPK"
     gene            complement(22564..25491)
                     /locus_tag="VHE33_12060"
     CDS             complement(22564..25491)
                     /locus_tag="VHE33_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT98232.1"
                     /translation="MGNNKMGIVSRQRVAQLIAVLVVPTAFGFQAASAQDAESVEAVT
                     VTGTSIRGIAPIGSNVITVDQAAIKASAPASMVELLSKIPALSSSGGAPQGQGYANYY
                     APNIHQLAASASNSTLVLMDGRRLPQGGEQHSQTDPGIIPVIALERVDVLADGASSIY
                     GSDAVAGVVNFITRSHYSGVQLTGQAGYANGYQTWNASYLVGKDWDSGWIMAAGSHSF
                     QSHLTNAARPFTAVSNFVAFGGLNNASFNCSPAAIKPAGSSVIYPSVTSTTPVANTSA
                     NAPCDYAGQGDDLPQEERDNILIKGSQQFGRVTLSADIVYDVRQDFKRGQPFSPYAPG
                     TVTSTVFATGPQANSFYMNVPGSTATSETVYWSASGFVPLALNEEGANTFYANAKAEW
                     NIDDNFVLTVTNSLGKDYTFSNVYNLFCASCANLALNGTTNGSGSLTTPSIAGTQVYA
                     TNLPLNASNELDVWNPPGTSNRTSAAVIASLTGNSFTNNLNNFDQLTAGLNGNLFSLP
                     AGPVKVAAGLENFHAALIQQVIAPNGTGTSQKGSLAAEYRFAHTVQSEYAELAIPLIS
                     PSMNVPLVQKLDIDLSGRHDEYSDVGGTTNPKASASWQIIDGFKLVSTYATSFVAPSL
                     DSLGDPSQYYNAAQTQVGDYATINGVAFNIPTAAFPGIAGVLPGCAATATVCQAGTAA
                     VPGITFYRGGAKTLKPQTGNSWSVGADLAPSFLPGFVVQITYFNNKLKGGVLSPSVGA
                     EVSTPALQPKFTVCPTGCTQAQIDAKTAGLPLKSGTMPTVAYFLFDFSQANVLYLDIG
                     GIDLNGQYSFDTAMGTFTVGDSLTEFTRYNYAAGQGVTSFSILNTAGYNANFPSVQTR
                     NRLNLGWTDSAFSIDAFVNFTGSYRMWGPGTVNPLTFDANHNPNGGGDKVNSLTTLDL
                     HAAYDFAGGILGGDQIYIDAKNLFDTAPPFIQGSTAGGRGYDRTVANPLGRVVSVGFS
                     AKF"
     gene            complement(25719..26657)
                     /locus_tag="VHE33_12065"
     CDS             complement(25719..26657)
                     /locus_tag="VHE33_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98233.1"
                     /translation="MAYIKLFALVLIAADLCGSGLAAPTDIVIDDQKVFPESMGATPD
                     GTIYIGGSGTGKIYRAGPMQAYAEPWISQKEGDLHFVLGVLPDAAANILWVCDNKMDD
                     HTATLKSFALKTGKKLNSYDLPGGGLCNDIAIKGRTIYISDTIKGRILKLASGAATLM
                     PWYTQDPSDPTLDGLVWARDGKLYTNTYVGNHLYRVDVNKDGTAGKGTMLTTDLPLYQ
                     PDGMRLSTDGRILMIEGQGRPGAGLKEGRLEEVLPMGDTAKIKVLKSGFELPVAVTPV
                     GNTAWVLESKFDFQRNDDLKGKDPGSFHIYAVPLGR"
     gene            complement(26707..28002)
                     /locus_tag="VHE33_12070"
     CDS             complement(26707..28002)
                     /locus_tag="VHE33_12070"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HVT98234.1"
                     /translation="MKSRLLEVCVVCLTILPAGVIAQTAPPTGTPPGVKPLVPDKILT
                     DFNAVLVEGQPIERRPPEKADDKPAFPGQTRAPFHRTAPFNVKILTEKLRSPWSFAFL
                     PDGKVLITEKPGSMRLLDSNGTLSKPVAGVPAVFNFGQVGLLDVALDRDFAANHRIFF
                     TYSELVGDTDSNIVVARAIFDEAADALEKVEVIFRARPALPAKTLVDNQGGRIAIGRD
                     GNLFVTIGDRSNSPPWDMAQKLDTDLGKIIHITPDGAPAAGNPFIGKAGALPEIWSYG
                     HRSEEGLAIHPVTGQLWENENGPRGDDKLLRPRAGANYGWPVIVHGIDYPGETIGEGI
                     TEKEGLEQSLYYWDPVIAPSGMAFYNGNLFPQWKGSVFIGSLRGRMLDRLSLSGDKVV
                     NEEPLLLDMHNRVRDVRIGPEGAVYVLTDNGKLLQLTPK"
     gene            complement(28314..30455)
                     /locus_tag="VHE33_12075"
     CDS             complement(28314..30455)
                     /locus_tag="VHE33_12075"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVT98235.1"
                     /translation="MMMSWSFVTGAAALIGLGSALAWAQTAPVGVYTAAQAAAGGTVY
                     AQNCAGCHRANLTGGGDAPALGGNGFISSFGARSTKDLYKFIATSMPAGVPGSLSETQ
                     YTDVTAYLLQANGAPAGSKSFNKSTDVKVFSIATGKPPVRAPRAAKTANDAPLNQPTD
                     QMAPAFQLGQTVKGTVKNYVDVTDKMLTHPPDGEWLMYRRNYQGWSFSPLKEITTDNV
                     KSLTLKWAWNMNEGGASQVTPIVHAGVMFLSNTSNTVQALDARTGELIWENRIGPVSK
                     IAYGGTRSLAVYHDKVYVATTDAHIHALDARTGKQVWEQTLGNPNNSNTGGVMVMRGK
                     VLTGLTGCDNYSKNNCYISAFDAETGKRDWKFFTTALEGQPGGDTWNGLPNLLRGGGD
                     TWIAGTYDPELNTTYWGVAQTKPWMRASRKTGNAATLYSSSTLALDPDTGKLKWYFQH
                     SPGESLDLDEVFERVLIDHGDQKTLMTIGKVGLLWKLDRESGKFLGVKQTIFQNVFSG
                     IDYKTGALTYRSDILNQKTNEWLSSCPGPEGGHDWQATSYDQPDDLLIIPLSQSCVMM
                     EGHEVEMKLGGGGTAASQKFFFMPGTHQNMGRLSAYRTSDMKEVWSWQQRSPFLSAVL
                     STGGGLAFVGDFSRSFKAVDVKTGKIIWQTRLGNTVQGYPVSFSLDGKQFIAVTTGLG
                     GGSPQLKPAALLDEVHRAQTGNMLYVFGLPD"
     gene            complement(30452..30826)
                     /locus_tag="VHE33_12080"
     CDS             complement(30452..30826)
                     /locus_tag="VHE33_12080"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HVT98236.1"
                     /translation="MAVTQIFEAELDPAPFDATNSADVIGSIGNITASLEGNILTIQG
                     NFSRMASPATAASVRVGLAKGVPGEAIGKLTVAHAPEGQVSGSVALTQDQVAALQKQS
                     LYIRIDSAKAPEGNLQGWLEGK"
     gene            complement(31019..32665)
                     /locus_tag="VHE33_12085"
     CDS             complement(31019..32665)
                     /locus_tag="VHE33_12085"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HVT98237.1"
                     /translation="MEEDTVRRCIITLILLSAACTLSPAQARPLPELVTHGARHALLV
                     DGQPFLMLGAQVDNITFTPQEMAKVLPGFRQHHANTVEFPVPWKLIEPQPGRFEFGAV
                     DQIIRDVRGQGLRAIVLWFGTWKGDESQFLPDWIRDDHVRYPLAVDANGQATKSLSPH
                     GEATLRADRRAFSALMQHLRIVDGVDHTVILVQVENEPGIVGSARDHSAAANRLFDAP
                     VPRAFAGALHLKPGSWTQAFGAGVDEEAFTSYYMARYIDSVAAAGKQAYPLPMLVNSW
                     QGGAGTGDRYYDFDRPGETYPSGGGQSHTLDWWKAAASAIDLIAPDIYNRSLSINRTI
                     MNRYARRDNPLLLVEAGRGLDFARYCFMAIGEYGAMGCAEFGAGIKLFGASDDGQWGP
                     DFVEMAANYGLLSNAAPVITPLLGTRKLQSAIESENIAGRQLSFDRWDVLAIFPPAKS
                     KRPVWMDLEGTPAPPSGRVLVAQLATDEFLVFGFDAVVDFRPPAASGRDSGRFTLIER
                     GRYMGGVWTPDETLPGATPARGAVVLPASGAMYRVKLASN"
     gene            complement(32734..35331)
                     /locus_tag="VHE33_12090"
     CDS             complement(32734..35331)
                     /locus_tag="VHE33_12090"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT98238.1"
                     /translation="MIAWGNTIFGGIAMKADRIVFTLAAAASCALLSGLPASAQQDTA
                     NGTTAQQGTTKDTAKDTAKGTLEEIVVTAQKREQNIQDVPSSIVALSGKEILNQGSVD
                     IQSMSQTMPNLFIRHNLTGDQLLMRGIGTGVDNEGFEQAVAQFVDGVYYGRATLDQNE
                     VFDVERMEVVRGPQPTYAGQSATAGAISIYDRKPGKTLGGDASVSYGKFNELSVNAGI
                     GGPVTDTFGIRLSGRFYDLPNTDYHNYDGSAVGNKRDWAVRLITTWNPTDTFDFTFKY
                     EHQSTFNFGQGGSYSICEMRPQYSRGNGFLDPGMPAACALNVAVLGADLNNRHQAYQG
                     GSLDARAAVDALNAASGAAPGSPGYWGYNSGPGVTGLQAIPYGLNKVKEFAQPQNRTY
                     NFDVLMGNFDWQLGDLTLTSTSSFLRYSKNYILDPDYSAFALFSDHRAEKFQQTSQEF
                     RLTSPVAQTVSWMIGASYLHHNLETTINVYAPTNFGAPFLPPTATAAGFGGTLFESAD
                     WYNAFAAATWNVTKDFRLNLGGRYQNVSKEGRLPATYAYLLPGAAAFSDMLPFPGGAQ
                     AVGNLKNGDFLPEASLQWDMNDNVMAYVKYSNAAKAGGFVMSPIFFGGVPNPFTYKPE
                     RAQGFEGGIKANLLGDSLQLAVDYYYTKYKNLQVSVYDAPSNAFITTNAGRSHTQGLE
                     FDGRWLPIPDQDFTIGFTGNLFGDASYDEFNGASCNQLEAKLIPAQCAVGVSRNGVAL
                     PYSSKWQVGINPNYQFSVESGYRITLDANLLRNSGYNDSDQQDPLDHQPAYWRIDTRV
                     ALTPENGPWELAIYGRNLTNTRVLLQSQSNFMATTSDPATLRDTWGPNLDFGMTFGAQ
                     VTYHLGSED"
     gene            35898..36356
                     /locus_tag="VHE33_12095"
     CDS             35898..36356
                     /locus_tag="VHE33_12095"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVT98239.1"
                     /translation="MTSRAPAPEISVPGEPVSVTIAQNQKELLASPDSQLAANKRLVF
                     DWWREVLVAAHVEVADKYMHADYIQHNPNIATGRQGFVNFFSARPRQEISSTIANLVT
                     IVAEGDLVVFSFRRELPDPRDSTKRYTSTWFDMMRVKDGKIAEHWDYGTK"
     gene            36461..36985
                     /locus_tag="VHE33_12100"
     CDS             36461..36985
                     /locus_tag="VHE33_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVT98240.1"
                     /translation="MNFFAKAISSVAVVACLGAADLAFAAPAESTSATASDPEGAPPD
                     PGHVPFVLPKDIKWIGTPGKQETAFLYGNPDKAGPYAVLYKWYPGAFSHPHFHDRTRW
                     IYVVSGTWWVSSSNTYDEKLTFPFHAGTFSTDVANTVHWDGNRAGEQENAVILLAGIG
                     PVKTIQVDEQGRPQ"
     gene            37192..37800
                     /locus_tag="VHE33_12105"
     CDS             37192..37800
                     /locus_tag="VHE33_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98241.1"
                     /translation="MAAMDCKITRVRRLLHDICGNPAVHARFLNTLSLLEHAGSRKIM
                     LSQSRPGASLDHETLKHLAEETRHAYFFRRAAEAVARRKLDYSENDLLAGAHARLYMG
                     RLDAFITRSVQGPLAYLYMTLVVELRATWFYNLYQDVLKELEQPINLKGLLAEEKRHL
                     EGISMQLSALGEDINVSLPLLKSFEESKYAALLGALERSANI"
BASE COUNT         7857 a        11172 c        10896 g         7960 t
ORIGIN      
        1 acattggccg ccggataccg gctctggtcc agcgcgatgc gaccttgtcg aacgccacac
       61 gcgccgaaca ggtcggcgca tcggcggcat cctgctggcg acgcatgcgc gccattcgct
      121 ggaggcagaa aagcggttaa cgccacgcac tcggaccaac ttggttttct taagaaactg
      181 ccaaagaaac tgccaaggcc cagcgataga gggtagtttg gccgcagagg cttcgccagt
      241 caccgcgcaa aatccgtgtt tccactgact tttttggtct gatgcatctc ggggtcgcgt
      301 ttgccatctg cgttcttggc cgaggatggc gcggcgatga tagtcgcgtc cataacggta
      361 cccgcgccaa ccttgatccc cttggccgag agatgcttgt tgaccaaggc gaacagcttc
      421 tcacccagac cgtggacttc aatcagatga cggaatttgc aaatcgtcgt ctcgtccggc
      481 gtcggttcgc ggcccagatc gatcccaacg aagtcatcgc ggcgttgtca tatagcgcat
      541 cttccaccgc gggccgccgg gcgataaaac ctgaattttg tggcccttac ggcatacgca
      601 gcaggaggaa ggcaagaaag acaaccgcca cacgaccctg cgatacgcat tcgcatgccc
      661 tgtcgaactc cgagccctgc gtcggccaca tcgaaattcc gcgacgcgtc ctttgagcat
      721 cagaacccgc cggaactttg gggcaagagc ggggaggaca gaatcctgtc ctcccacgct
      781 ttgactgtct tacggattga tgatgctgcg cccaaccaca tgctctttca gcagcgaact
      841 cagaggtacc atgcccttgt agatgaactt ctgcacgcgc gcaccgtcaa aggtttcggt
      901 cgtgaagatg ttgcctttgg agtccgtaac gatgctatgc gcgccataga attgacccgg
      961 ttggcgaccc ccgccgccga aagaatacaa ctccttcatt gactggcgat caaagacgtg
     1021 aatctcctcg ttggaaccat cggtgatgta catgaacttc tgcgcgggat ctttggaaaa
     1081 agcgatctcc caaaccgagc cgagggccct ggtgtttacc gccacaccat actgtttcac
     1141 gaaggttccg tcgaatttga agacctggat gcgatcgttg gtgcgatcac agacatacag
     1201 cagaccatcg tttgacggct gggcgcaatg caccggatta ccgaactgcg gcgaattcac
     1261 attgtaatgc ggaatcggat cgtcggtggg cggcttgcca tacgcccccc acatgcgaat
     1321 gagcttcatc gtgaccggat cccagaccga tacgcgatgg tttccatagc cgtccgccgc
     1381 aaccagttcg ccggtcggca ggaaacggat cgtagccacg cccctcacat tatcggtatc
     1441 gaggcttccc ttgctgccgt tagcatgccc gatttcaccc aggaacttcc cgtcctgaga
     1501 caatttcaga agaaagctgt cgtgatactg gcctttgacg aactcgccgc cggtttcttc
     1561 gtcaaaggcg cctccattcg gatgcttggc gaactgttcc gcgcttccgg gaggaggacc
     1621 gggttgggca gcgctagcgc ccgccatcca cacattgccg tctttatcga tcgtgatgcc
     1681 atggttcccc gccggccaat catgtccttc gggcgggcca aagtaatgta tcaaattgcc
     1741 ggcttcgtcg aattccagaa cgtccggagc cgccgtgcag catttcgctt cgcctctggt
     1801 caaactggac gcatttttct caagcgatgg cggcctgtga aggacccata tattttcatg
     1861 cgcatccatg gatatgccga tggtctcgcc gagcatccag tgattgggca gcggcttagg
     1921 ccatgaggga tccacttcga atttgggaac catcgtgctc ccaggcacaa tgtcctgcgc
     1981 ctccgcgacg tcgcccttgt cgaacacaga tgggccaacg gcaaaaccgg cgagaagcgc
     2041 agcgaacgcc ggaccagtca gcagtttgcg cgaccggatg atatttctga ttgtatattt
     2101 catggagggc tccctggaga tgggaggaaa ttgcctacgc ctcccttttg cgcgaaggtt
     2161 tacattatcc cccacatgaa gcaagccgcc ggttgtgtcg gtagtgggtc cgtttgataa
     2221 ataccccccc cggggacttt ggcacggcgc tgaaaatcag ccaaagccaa gtgatttcag
     2281 accgacccac caccgtagtg tcctgatttg attttcatgc ttgccttggc ctgcacccgg
     2341 ttttgaagac accgagtagg tgtttttatg gaaccgggag ggggtctgga gagacggaag
     2401 tttacgcgag ggaggctagg tgcctacttt gaaccgcgag tgacctaatt cggccactac
     2461 cggatttagc ctatgcttgt gtcgttggga atcccggatt agacttgaaa tgtcagctta
     2521 tgggaggcga tggggagatt tttcatgagg tattcgggtt tagaggaggc ctgatgaaaa
     2581 agtttctcat cgcgtcatgc gcggtcctgt tgctgcccac cgcggccttt gctgatcacg
     2641 atgccgaaat gatcatcgct taccgatata gcgatctagc gttcagggcg cccactattg
     2701 atttggtgca tatgcatctg caccaagtgg tgaactgcct ggtcggcccc gggggccccg
     2761 gctttgacgc cacttacacc aacccctgcg ccgaagcagg caatggtgcg attcccgatg
     2821 cccccgaatc gtccatggag ttccaaatgc ggatcgtcct ggccacggtc aatgccggcc
     2881 tggccgccac cgatattgcc accgccaaaa agtacgcggg cgatgctagc gcgaccatca
     2941 acagcggcca gtgtgcgctt gcaacaggtg ttcactatgg ctagctgaaa aagtcggtgg
     3001 tttgtgggct tttggatagt agaagcacgt cgggcatcca tactgctttt gcgtcaaatt
     3061 acgtctcaaa cgcctgggac aatcaaggag cttttgatgc gcatctcgat tgctatgttc
     3121 ttcttatttt ttgtaagcct aatccccacg gtcgcagcaa ctccaaatat ccgcgttgat
     3181 ggtgcttggg ctgcgccgac cccgaacggc gcccagacgg gcgtcgccta tatggtgatt
     3241 cacaatcaag gtcccaatga tgacagactg gtggggggaa cttcaccagc ggccgacaaa
     3301 attcaagtcc acgaaatgaa ggtcacgaat gatataatgg aaatgcgaga ggtcgtcgac
     3361 ggccttcctg ttgcggctgg tcggacagca gaactcaagc caggcggctt tcacatgatg
     3421 ctaatcggtc ttaagcggca acttcgagcc ggacaagata tcgttctgac gctgagattc
     3481 gagcatgctg gagaggttct cgtacacgtt cctgtcagag cggacagcag gcgatgacat
     3541 ttcaatccct gcgaactacg ccaagcgcgc ataagggatc ctacatgagg cttcaacgct
     3601 atattttgat ggcgggggtc attgtggtgg cagcgaccac cagctcggca gcgacggggc
     3661 tgccaacatc tcacatctca aacaaaccaa cgcctattcc cggcataggc ggtccgttca
     3721 atttgatcga tgacaatgga aggaaagtct cggcggcaac attagccgga aaaccctctg
     3781 ccatcttttt tgggtatacc ttctgtcccg aagtctgccc gacgacgctt caggacgtga
     3841 cgagttggat ttcagcactc ggctctgacg cgggcaagat caactacgtc ttcgttacga
     3901 tcgacccaca ccgcgacaca ccaaaggtaa tgcaccaata tttatcttca tttgatcgtc
     3961 gcatccgcgg gttcagtggt acagacgaac agacggcaaa aatagcaagg gaatatggcg
     4021 cctattacaa gcgtgttcca gctgcaggcg gttcatacgt catgaaccac tctgacagaa
     4081 tctatcttat gaacgcgcaa gggcaatatg tcggctcaat ttcctatcga gaaccagatg
     4141 caagcgccat atcgaaactg aaacaactac tgcagtgaaa gtttcagagc ttgacttaaa
     4201 ttgaccgagc ggttgaaaag gtcgccaata tgacggaggt ctgacgaaga tcagataacc
     4261 gctctgtatt gagaggtggc gtcgggattg aagcgggagg cctgcctgcc gcttcaatcc
     4321 caagccggat taagaccgcc tacggcgccg gtgcggccgc aggagcagcc ggccgcggcg
     4381 cagccggcgg gttcttcagc caacggaccg aaccaggaat gttggtgagg ccgccataga
     4441 tgatgccgtc atcgtcgacc gcaacagctt ccagcgaggc gaattccgca taaggaatat
     4501 agcccgtcac ttttccgtcc gacacgctgc cccagcgtat gccgggcgtg gtaattccgc
     4561 taccagggtt gcttttgacg gtggagttga agtcggagac ataaaggatg tcgttcttgt
     4621 cgatgaagag accgctgggc gatccgaact gcttccagac attcagcagc ttgccgtcct
     4681 ggtcgaatat cttgaccgca tggttgttac ggtcggcgac ataaagccgg ccttccttgt
     4741 ccatggccag gccatggggc gggttggtgt cttccggtgc gctgccacgc ggggcgaatt
     4801 ccttgatgta tttgcctttg ctatcatatt ttatgatgcg attgttgttg gtaggggaac
     4861 catggccgtc cgcgacgaag atatcgccgt tcggcgccag caccacatcc gagaggccgt
     4921 tatagacata ggggccgtag ccggccacac cgtctgtgcc catggtcagc agcagcttgc
     4981 catcggggct gaacttgcgg atcgtgtcgc cgatgcctcc ggaatagttg atgctgtcca
     5041 cgacccagat attgtcgtcc ttgtcgacgt agatgccgtg cggatcgtga aacatcccgg
     5101 cgccgaagct cttcaccagt acgccgttgg cgtcgaattt cagaatcggg ttcacgtcct
     5161 tgtgcagatc gcagccgtca tcggtgttgc cgcagcgttc gaacacccac acgcttttgc
     5221 cgtcgcggtc gatatccacc gccgacgccg cgccccattt gcggcccatg ggcagctgca
     5281 gccagccatg ctgaatatga tagggcgcga gcggcccgtt attttccggc gtcgcgtcag
     5341 cgccctgggc caaggccatg gctgtcgttg tcaataacat cgccgtggcg gcaagcagcg
     5401 tggcaggtgc ggcaaacttc ataattttct ccaggagaag cagcgcgact aaggcgcgct
     5461 tgtttgtgtg gttatattat ggaccagcag aaattgatga aaactgctcg ggttctgttg
     5521 gccggcatct tgctatctct tccggtattt gtccagattg ggcagcaaat gggggtgggg
     5581 cctcgacccg ctatccggct gttgcaggaa tttgcggtat gcgcttcaga ggcggaatct
     5641 cacgcccatt gcgtgagcaa tcaggaaata tgatggccgt ccccaatgcc ggacgcctcc
     5701 gaccacaggg ttgttggcct ggacgggcca caggtaattt gctgcgagtg cgaaccgtgg
     5761 cgttgccggg ttctgtgcgg gcgctgtttc cctcgcgcga tttttcgatt atcgtggcgc
     5821 aataaaattc attggaggat ctcgatgcat atcatcgcca aactttttct cggaacggct
     5881 cttctcgccg gcgccgtcac gggagctctt gcccaggctc aaccggggga tccgcccata
     5941 ccaacttata atgacccgcc gggcccgctg cccaatcccc tcaacatcac gatgcatccg
     6001 ggcagcgttt ttcctgaaaa cagagtgttc ggcgatccga gcaagccggg gatctatggt
     6061 atgatcacta catggcaacc cggaaagttc agccgtccgc atttccataa cacggaccgg
     6121 tttgtctatg tcgtttccgg cacgtggtgg gtaagtgcaa gtaacgattt cgatccgaaa
     6181 acgacctatc cgattcccca aggcaccttt gccgtggacc tcgccaataa ggtgcattgg
     6241 gatggtaacc gcctcggcgg cccgccgact gtgctttaca ttgtgggcga gggtccattg
     6301 acaaacatca gtgtcggcca tgatggcaag ccgttggcgg ttcaaccgaa ccaccaacaa
     6361 cggccggctg ctcctgcagc cgcaccggcg ccgtaagcgg tcttaatccg gcttgggatt
     6421 gaagcggcag gcagacctcc cgcttcaatc ccgacgccac atctcaacac cgacctgact
     6481 ggctcttttt gtaccaagcg gatcgttaga aactctcaac gcagattccc cgcaacggga
     6541 tcgttatctt atctatatcg cgtttagcct cgcggttgcg ctgacagcgc tatgggtcgg
     6601 ccaccttacg gcttatgcgg tgcgggccgc atctcccaaa agcccgctcc gagtggcgcc
     6661 atcgcctgcc cggtcgcgcc gcgacttttt ttccgacatt cggcaaagtg tttgccggcg
     6721 ttgcactggc gaccgcgctt ccgagtctgg ggttcgcaca ggaccgtttg cgtgactgcc
     6781 tcacctgttg cgcgaaatac cagaattccc gcgggaatgg cggcgactgc aatgtgaatt
     6841 atcaaaattg cgttgcgaac tgtaacaacc aaggcgaaac gccgtccact tggcggtgct
     6901 ggtagcccca agctacggcc gcattgacca cccataaagg aatgaccgct tccggcgcaa
     6961 agggggcatc tcttccgtcc ggttggaaca gctggctgga tcgggatgag aagaggaacg
     7021 cgtctgcgta tcgcagcgcc aacttattga aagaatggtg gagcagccat tacggctgaa
     7081 ccagtctgcg gcctaacttc gcccaaatac agggaaaaac cggaaaatag gcgaaaatct
     7141 tctattcacc ggcgacataa gcccaatttt actggctttt cacggccaaa cgcccctcac
     7201 aaagaatagg gaaggcccac cccaatatca gggaatgatt cgccccgtat cagggaaact
     7261 ccggggcggg caggagcccc gtggtaccgt ctctgcaact gtccctcagg gccgtccaaa
     7321 tcgttctggg aggcaattag gcggtcgagg aggccctagt ggcagcttct ctgcgggggg
     7381 gcacccttca agctcggccg tctgcccgct tccgagccat tcggcgacgt atggcgcgca
     7441 ccctgttcgg agcggcctcg aatttaacat gaccggtaaa cagcatcctg cactgctggg
     7501 cagcactttt ggaaagctgt gcatctgttg ttcttgcggg ttacttacgc gagccatacc
     7561 cggttgtgat gagaccggtt gacagcatgc cgcgctatat ccgcggaaga cggaatgcga
     7621 tcagttccgc cgaatgatcg ccgccaattc ccaggacgat gtattgacgc ccatccaaca
     7681 tataggtcat aggcgcgcct gtctgcggtc cgggcatata gaccgcgccc ctttccgcgc
     7741 cagtcgcctt gtcataagcc cgcagcatcg cgccgcgtac gccttgcggc gaaatgccga
     7801 agcctgcctc gccgcagatc aacaatgtct ttgttacagt cgggcccagg atacctgttc
     7861 ggccagtgcg cggaatcttg agacccttca gcgcggggtg attcttgatg ttgtctggcg
     7921 tgtcaccatg ggcgacctgc caggtgatgt ctccgttgga aaggtcgatg gcggaaagag
     7981 tgccataagg tggcttgagc agcggcagac catcgatggt cgtcagacca tcgggagtga
     8041 caggggtcgg gccggccccg ccccccgtgg tattttgccc ggtcaagcgt tgcgcgcttg
     8101 gcgtgccatg aacatattgg aaatccgttt ccttggggtc gcctggcacg atgccaagta
     8161 tagcgaccga attctgggcg aatacgtaga ccttgtgcgt ctcagggtcg taacaaccac
     8221 caggccaatt ggtgccgcct agcaggctgg gcgcgcacag cgaaccccaa tttccaccca
     8281 cagttgccag ggtcggcgga ttgaagaccc ccccggtgcg aaaatgggaa atgaattcca
     8341 accctttggc gtgcaattcc ggggtgaagt caatgatggt gtcggaattg acttcctgca
     8401 ccccataggc gggcggcctc gacgggatgg gttgtgtggg tgaataccat tcgcccggta
     8461 catcgcccgc aggcacgggc gtttcgggaa tcggccagac cggctcgccc gtctcgcggt
     8521 ttaacacata gaggaaagcc tgtttggtcg gctgggccag cgccttgacg atcttccctt
     8581 catacgggat atcgcacagg attggcgcgc aaggaatatc gcggtcccat aatccgtgat
     8641 ggataagctg gtagtgccac ttgcgccttc ccgtatcgat atccactgcg acgatgcttt
     8701 cgccgaatag cgccggccct tggcgataaa tgcccacctg atcacccgtg gggagttcaa
     8761 tcggcaggta ggctgtatta agttccagat ccacggaaat ctggccccag caaccggtgt
     8821 tgtccgcccg ctccgcatcg ccagccttaa tccatgtgtc gtaaccaaat tcgcccttgc
     8881 gtgggatggt atggaaaatc cattttcgct caccggtgcg gacatcgaag ccgcggacat
     8941 agcccttcac cccgaaggac cttttcggca catcaccggc cgggtgagcc gcgcccacga
     9001 taacgacgtt cttcgccacg atcggcgtgg cgtggaggcc tacctggcca ttgaccaggt
     9061 cgatgatttg gtcatcattt tccttcaggt cgacgatgcc gtctacgcca aaacttcgca
     9121 ccggcgcgcc ggtatgcgcg tccagcgcga tcagccggta gcccgttgtg acataaagga
     9181 tgcgctcgtc tttcccgtcg gtccaataag cgacgccgcg accggaatat tttcgtggcg
     9241 acgcgtcagc gcgcgcgccc tcatcctcgc tgtgcttcca aagtatttca ccggtagcag
     9301 cattcaggca caccacggcg cggcgcgacc cggccgtagc atacaatcgg cccttgacga
     9361 ccaaaggcgt gcattgctcg agaaattccg gatagggacc cagggaacca gccttgaacc
     9421 gccatgcgat ctcaaggtcg ttgaaattgc ccgcgtgaat ctggtcgagc ggcgcatagc
     9481 gggtgttcgc ggggtcgccc gcgtaatttc gccattcggt gtcgggcgct ctggcttgcg
     9541 cccagccggg gagccaagac gctagatagc ctccaatcgc cgtcaatagc aaagtcctgc
     9601 gagacagcga tttcatgaca ttcctaaaga ccggcggtca cgactatgac tcgccaattc
     9661 gctgcaaacc cacaaaccgc tgcccccatt gacgcaggca gagcgcgaat gatttgtgtt
     9721 tcagcccatt gccttcagtt ggctgacacc ctttctgatc ttggctttca ggtcttcgcc
     9781 gatctctgtc cagggcgggc gcgtgaaggt tgttcccaaa tttcccatca ttaggctaag
     9841 cgtgtatttg atggcgctgg gattataata atcgccacct tccatgaacg acgtcgccct
     9901 cgccatcttt gccagcggcg cggtcaagtc cttgcccgcg cgatgggccg caaagatcgc
     9961 cgcaacgtat ttcggataca cgttacccga tccaaggatg acgccgtcgc cgcgcctcaa
    10021 ggctgcaggc atgttgttgt cagtgccgga gatgatattg agtctcgctg gcaggccgtt
    10081 ggcgatgacc tcatattccg ctggatcgcc gtgcgaatcc ttgatgccaa tgaaattggg
    10141 atgctgcgcc aaggcttgaa ataccttaag gcccaccggc acgccggtaa cctggggtat
    10201 gtgataatac cgcaccggcg tccttacctt gtcaaagata gcagtgaagt aacggatcac
    10261 gccatcaccg gctgggtcct tgaagtagaa gggcggctgg atcagcacac tgtcggcgcc
    10321 gatggccgcc gcgtgctgcg agagttcgat ggtttcgttg aaatttgcgg tcccggttgc
    10381 gacgatcatg tcgagaccca ttttgttctt aagcgcaaat tccgcaatct tcttgcgctc
    10441 tgcaactgaa taggacgggc cctcgccatt ggtgcctaat accagcacgc cgtcagcgcc
    10501 attggctttg taccaggaca acaggtcgcg ataggctccc tcgtcaaagt tgtagctctt
    10561 atcgcagggt gtgaccgcag caacccagaa tttgtccttg gccattgagg cgtgagccgg
    10621 cgccgcaagt gcctggtcca aacccatagc ggcggtggcc gcgcccatgc ctacaaaaaa
    10681 cctgcgtgtc gtttccatct gttttttcct tatctggcct aggccactgg ttggtgccaa
    10741 gactcttgaa atcacctagg ggctgtgggg cggcgacggc attttaatat gcagaacgtc
    10801 gcgcagataa ttgacgtttt ttgcaagagt gtcctcgacg gtgccctcgc ccggacgcgg
    10861 gccgtcgaca tcgaccacgg cccagccctt ccattttcgt ttccgaagca catcaaagat
    10921 accaggaaaa tctacgccac caaactcgcc catcgtgccc cagatattgc ctttcagata
    10981 ttcggaattc gccagtgtga ccttgttgcc gcgatacttg gcgtaggtgt cctttaggtg
    11041 gaactcggcg atgcggggga gatagtcgtt tacgatcttt acggtatcca tgccaccgag
    11101 cacattgtgc ccagtatcca gcgtcaacca aacatatcga ggatcggtca gttccattac
    11161 cgtgcggaat tcatgttcgc gttccatcgg tccccagata tggggatgag gtgcagctcg
    11221 gacgccgaac gcgatggtct gccggccgat ttcgtttaag gttcgcgcaa gagcctttag
    11281 ctgcgcgggg ctcgggccgc ccttgggtcg cccgcccatg ttcattttcc aatgatcgca
    11341 acctgcgggc gccaaattgg tgcgggcgaa ctgcaccatt gccgtgatga gctcggcgtt
    11401 gccctcctcg cccaaccaag gataaggacc gctcccaaag gggttggtgt ccttgaccac
    11461 gcctggcggc gtcggtgggg gctgcggtcc cagcgagcgc ggcatatcac caatgtcgat
    11521 aaactggatg ccggcgtcgt cgcaaagtct cttgatcgcc agcggcttgt taaggtaatc
    11581 acctatctgc gtcgagtaag gctcgaagcc ctgaaagccg tagcgcgccg tggccttgat
    11641 cattcccgga atgtcttggg aatccgtcat cggcgctccg cgcttgggcc aacggccaaa
    11701 ggcgccgcca gcaagtccga aacggattcc agggtttacc gggtcgaggg acgccattgc
    11761 cccccatgca ggcgacgcat cagtcatgcc tgcagccgcc gtaacgccga acacggccaa
    11821 tccgccgcta aggaattgtc gccgcgcgac tggatcgttg aaactcgtta ccatgcatgt
    11881 agtccttttc tgcgacgctg gcactttgtg acgcaaactc gtctgacaat agttcgccgc
    11941 aggccgtcat ggacccagtc gacaagtgtc acgcgctggc cgtttatagt atcaggctct
    12001 gcacgaccgt ctttgacggg tcggcaggaa aattggaaaa ttcagcgcgg ctctaagtag
    12061 gcccgtcgcg gcgataatcg cgaggcgtaa cgccaaaatg gcgcttaaag caccgcgaaa
    12121 aataggccga atcggagaat ccgcaatcgt aagccagttc tgttatggac tttccgccgg
    12181 gctccctaac aaggcactca ccggcccgcg ccaatctttg ctcgaggatg aagccactcg
    12241 ttgtcgttgc aagacgctca aacaccgtct ggacggatcg cgaactaatt ttgagcgcgc
    12301 gcgcgatcga ggatgtccgc aattcggtat cagccaggtg gtgacggata tgctcaatga
    12361 cggcgcggcg caattccgcg gatgcgccga ccacctcatt gccttccgcc cggctgatcg
    12421 cacgcatggc cagatcaagg ctggtccggc tgacgcaatc gcgccagtcg gcgtcattgc
    12481 taaggcattc gcgctgctgc caagcagtac gaagaaatgc cagaagcagt tgcgagcgtt
    12541 tcacatcgac tttcttggct gcagaccgct ccagatcaaa gcctggctcc cgcgcgagaa
    12601 tgccagcaat cggcagttcg acgacaaaca tctcataagg cgttaggaaa tctacactat
    12661 aattgtaatc gggatcgcag atcgctgcct cgccggggtc gacagccgca cagcgcccgc
    12721 gctggccgct aatgctacgt cccacggatt gtagatgcag aagaacagac ccggacgagt
    12781 agcgcggagc tccggtgatc cagcgctctg ctctggaagc tccggcccga acgcgcacaa
    12841 gattcatgtc gttgatcttg cagcgccaga gctctccgct aaagtggctt ttagacgtat
    12901 caacgacgca accggaaaac gcctccccaa caatggtatt ccattcctga aggttttcac
    12961 ccgccttcaa gttcgaatcc accgccgaat tctgtatttc cagcatgcgc accccccatg
    13021 tttgggtttg ttgcgtggcc acgttgcaac tattgtgcaa tcaaataagc gaaaggtcta
    13081 gtaatcaaaa ttttagcccg ccttgctgca ctgcagcaat tcggcttcca gcttaaatgc
    13141 ccgcggcgaa cttccggcat actggcggaa taagcgcgag aaataggcag gatcatcgat
    13201 atcaagcggc gcggctgatc catgcccgct cgcgccgcgc cggcaacgcc tttgtcgcca
    13261 tcaactgcgc caccatggag ccgaaccggc cggaagccga attgttcggc gtggaatcga
    13321 tggaaggccc gcgcaagacc gccgcgtcgg aatacggaat ggatgtcgct ttcgtcaaga
    13381 aagtcatcgc gcaaagcgcc gtctataggg gcgttccggc agccaacgca gcgttgcata
    13441 tggtcgttcg tctgactgtg cagaaccccg gctagccaag tttcgtcagc cccagaattt
    13501 cacgcgcctc gccgggattt gcaatttcca atcccatatc tgtggcgatc cgcgccgcgc
    13561 gggcaacaag ctgctcattg gtggccagca tacccttgct ataataattg ttgtcctcca
    13621 gaccgacgcg cacatgaccg cccagtagca tcgcctgtgt ggtcgcagga agctgcgcag
    13681 ggccgatcgc tgagacgcag aagattgagt gcggcggcaa tgccgcaatc attacgttca
    13741 gtacgtcatg gctgtagggc atagcgccct gaaagccccg gtgggcgccc agcaccatgt
    13801 ttatgtagta gggcggcttg tcgtaaccct tttggatcaa ccgggtcacg tcctgaagga
    13861 tgtggttcgg cgcgaatact tcccattcgg gcttgatgcc ccgctcagtt atccgcttta
    13921 tcaatgtctc gcagcggctt gggggcgtga ttacaagaat ttccttgccg ccgtgcacat
    13981 cgacaaaagt catgccatca aaggtggcca tttccgcgcc cgcttcgcag cccttgaggc
    14041 gttcctcgaa ccggttctcg aacaggccgt cgggccgttc gaatagcatg tcgccgctcg
    14101 atcccccgcc ggtggaattg ttgagaatga tgtcgcagcg ctcgcggatg cgcgaattaa
    14161 tatcgcggta gatgtcagcg ttgcaggtcg cctgatcgtc cggccgccga gcgtggagcg
    14221 ccgcgatgca cgcaccggcc ttaaatgatt tgtgcaccga ctcagcgatt tctccgggtt
    14281 gggtgggaag gttggcgttt tgcgccttag atgccatgcc gccagtcggc gcgaccgtga
    14341 tcatcagttt agtagaggcc atgattctct ccagcatttg cgctttgttg caaaagagat
    14401 tcccggactt cctgggggac ggacactccc cggatgctat ccggctcgtc cgggttctgc
    14461 tcggcccaga cgcgtttttc ctggcattcc gccgccaaca cgtcaccgcg catcaaccga
    14521 tggtcaatct cgacgctgct tccgccaaag cgccgaaccc tgctttcgat ggtcacgatg
    14581 tctccaaagc tactgggaac gtagaacttg gctcgcacat ccaccatcgg aaagccaata
    14641 attcgatagc gcttcagaat ctcgcgcttg gtgatcgaca gggccgcccc gaacagtgct
    14701 gccgtactca catcgaacat ggcgaagtag cacggataga acactatccc ggcgggatcg
    14761 caatcgcccc agtcgattgt caccgtccgc cgatttacaa acatttttag cgcccctcca
    14821 gtcgaaccaa cttgcgggta atgatttcgt cgatgcgatc gacgacatgg tgcgccatct
    14881 gtttgggagc cagagcatcc cacgcggacc gattggtcag cccgctaata acgcctatgc
    14941 cgatggcgcc acaaaggcgc gccatcgcca tttccaattt gggatcgtca ccgacaatcg
    15001 ccatctgaga ttcggcgcat cccaaacgcc gggcagccat ctgcgctgct attgctgaag
    15061 gttttccgat cacgacggca cgccgaccga ccgctttggc aattgcggcg ccgatcatcc
    15121 ctgaaatgcc aagcgttcgc cccttgctgg aatagaagaa cggaacatcg gaactcacat
    15181 acatcggagc gcctgctgaa acggcggcga ccgcagcttc aatatctgcc aggccgaatg
    15241 agggatgcca cccgatatac accgcgtcaa ctttgtgagg cagtaagtgc tctggccgaa
    15301 cgatagcgat acccacctgg gtgagcggac tggcaacccc ttcacccccg agcaccagca
    15361 ccgtgcggca gccggaagcg gcaaaatatt cggacgcgac actggaaggt gtcatcattt
    15421 cggacggact aagttcaagt ccggctgcgc cgaggttttc ggcatagcgt tccggcgttt
    15481 gggccgtgcc attggtaaag accacaaacg gcaacccgtg ggaccgcaat tcctttataa
    15541 agtcagcgac gcccggaagc agcagatatc caccgccctc cggcttcccc accgcgagcg
    15601 ttccgtccat atccaggatg aagcccttta cagtattcga gcgacgctta gacaagcgac
    15661 gggcccgcat gaagggaaag gcgaaaacct gggcgttcca cgacgatgtc gcgcgcggca
    15721 ggacgcgcgg ccaactccct tagcagggag tggaaaggcg ctgcctccgg caaatagcga
    15781 gccgaatccg gcccagcttt cagcatcgcg tcgacctgat cccccggcat gactgctcgc
    15841 aacagaaatt cttcttccgg catgtccgcg cgaaagcggc gccgcatatc ctggagaccc
    15901 agcacggctg gctcggccga aagctcccgc gcgcgcgggc ggtccagaat cgcggcttcc
    15961 acctcggacg ccacgggccg tgtgggccgc ccgaacctgc ccatgacata acgaataacc
    16021 tggtcggaca cctggctata tcgctttccg gaaataacgt tgaagagcgc ctgcgaacac
    16081 accatctggg ggaatggcgt taccatgatg ggatagccca aatccgctcg gacccgcacc
    16141 gtctcggcta taacctcggg gagcttacct tcaagtttga cctcagcaag ctggcgcaac
    16201 atcgttgtca tcacgccgcc agcgatttga tgcctgagaa aactagcatc aaactcctgc
    16261 ggcatcccgc gcgggagatt ttcggccacg gcaaggcggt tccaatagcg agacaccgct
    16321 tcgagagcac cctgatccac cgcaacagta tgaccgagct ccagcaaatt ggcgatcatg
    16381 cgtccagcct ctggcagtga ggtgccattg ccgagcggtc caataccgac atgcacagca
    16441 tcaacccctg cttccacggc ggcgaggctg gcaagcccgc ccaaaccgat tgtgcaatga
    16501 agatgtactt caagccgctt gcctccgatt acggccttaa cggcgggaac cagggtacga
    16561 gcgcgttccg gcgacatcag tcccgacggg tccttgagat agaacaggtc aatgtcggca
    16621 gaggcggcca gcagcgccgc aaacctggca tagaaagcat cgtcatgcat gtccgagagc
    16681 gtgtaggtca gcgcggccat tgtcgtggcg tcgccttctt cctttatgat ccgcgcggtt
    16741 tggagaactg cctggacgtc gtgcatcgga tcgagaagaa tgaaccgccg gataccgttt
    16801 gcctgcagcc gccggtagac gatccgcata aaatcgggat cggcctgctc ccatgcgatg
    16861 aagcgcaagc ccgtagtgat aaactgcagc ggcgtgcgcg gcatggcggc ccgcatgcgc
    16921 ctgatgcgct cccagggatt gtcttgaaag taccgcaccg caaccgccat gtggctggag
    16981 gaggtgaagt cgatagcgcg gaagcccacg cggtccagct gacccgcgat ttcaagcatc
    17041 tgcgatgtgc gcagccccgt cgcgccccat aggctctggt tgccgtcgcg catggaaaca
    17101 tcgaccagtc cgatctcagc catcggcaag gccctcgaag aatcgtgtat cgacggcgcc
    17161 cgcccggaac cgcgcatccg ccatgatgcg gcgatgcatt ggcgccgtcg tggcgacgcc
    17221 ttccaaaatc agcgtatcga gcgcctggct catgcgcgca atggcctggg tgcgatcaac
    17281 tccgctaacg atcagcttcc caatcaagga gtcataatag ggcggcacga cgccaccgct
    17341 ttcgatatgg ctgtcgacgc ggatatgcgg gccagcgggc caccgcgcag ttgtgatgcg
    17401 ccccggtgat ggcaggaaat ttcgtgtcca atcctcggcg tttatccgaa cttctatagc
    17461 atggccgtta agggtgacat cctcctgccg aattcccatg ggttggccgt ccgcaatcag
    17521 caattgctgt tcgaccagat ccacccctgt aatcgcttcc gtcaccggat gttctacttg
    17581 gatgcgggca ttcatctcga gaaaataaaa ctcgaagctt tccgcatcga caagaaactc
    17641 caccgtccca gccccctggt acgccaggct ttcggcaagg cgcacagcgg caagacacag
    17701 tgcggcatgc tgtgctggcg gaatatcggg ggccggagcc tcttcaacaa gcttctggaa
    17761 gcggcgctgg atcgaacagt cgcgtgtgcc gagttggatt gcgcggcggc catcgccgag
    17821 gacctggacc tcgacgtgcc gaccgcgggt aatataacgc tcgatgtaaa cgcgcggatc
    17881 gccaaacgct gcctgcgcct cggccattgc catggccacc atggcttcca gatcagcttc
    17941 gcgatccacc cgcttcatac cccgtccgcc gccgcccgaa acggccttta tcagtagcgg
    18001 atagccgatt tcagacgcat gtttgcgcgc ctcgccaaga tccgcagcct gcccgcctgg
    18061 aactataggc aatcccgccg ccgcgccaac cgcgcgggcc ttcaacttgt cgccgacggc
    18121 gtcgaggctt tcaggcgaag gcccgacaaa gatcattccc tcggcccgga cggcacgggc
    18181 gaaccgcgca ttttcggaca agaagccata gcccggatga accgcatcgg cgccggattt
    18241 caccgcagcg ccgacaacgc gatcaacatc cagatagctt tggccagagg gagccggtcc
    18301 cagtacaaca attccgtccg ccataagcgc cggcaacgcg ttgcggtccg cttcggaaac
    18361 tccgagtacc gtcgcgatcc ccatgcgttt ggcagtgcgt atgatgcgca gggagatttc
    18421 gccgcgattc gcaataaaaa gcgtcctgat catttcaaat gccaagcggc cggaggcgca
    18481 tcaggatggc gctctgatcc accatatcgc catgacctgc ggtgatctcg acgatttccc
    18541 cggagaatcc tgccggaata ctgttcatca ctttcatcgt ctcgatgatg ccgatgatgg
    18601 tttccgggct gacaatgtcg cccatcttca caaagggcgg ggccccaggc tgaggagtat
    18661 ggtaaaatgt tcctggcagt ggtggccgaa tcgcgagaag gccttcgact tcaattgctg
    18721 ccgcgggctc acccggtggc cctgcgatag gacgctcggg tggaattgga tgctgccact
    18781 cctgggtcca tcccccgccc tcagcacgcg caactttcag cacaaaacgg ggtgttgaaa
    18841 gctccaggcc ttgataaccg cttccattga gcaacgagac aatgctggcg acgtcgtctg
    18901 gggtgagggg gagatcgatt tcatcgctca ttcctttgcc acctgggggt tgcgcgtata
    18961 ggccaacacc tggccgatca gcgcatccgc aaattccgca tcgtttatgt ggcgatcgag
    19021 cgtgtaaagt tcgaccttct tgggcaacgc acgcgttaga tagtcggcga acacgggagg
    19081 tagcgatgga tcatgcaaat agccctccgg gctgtcgtga ttggaaaagc ctttgagcgg
    19141 aacatacatg ctgaccggac ctttggcgtt ttcaatcagg ccggccaaat gatccgccag
    19201 cttgtttaat tctgccgctt cggtgcgcac tgcggtaagg gccgcgttat gaagatggta
    19261 tcgcttgccg ggaaaacgcg cgcgggcatc ctcaatcggc cccgcgatca ggaagtccgc
    19321 gttgcctggc gcgaaaatta caggtacgcc catttccagc gcggctttgg atcggtcagg
    19381 gcctgcgcta cataagccgc cattgagaaa atcgttgatc tcgaccagcg acatgtccac
    19441 cacggccgcc accggccgct cgcgcacgat ctggtccata gtttggcccc cagcgccgat
    19501 ggtgtgaaac accatgactt cgtaacctcg cgcctcgagt tcggtacgta tgcggttgct
    19561 gcagcgttcc gtcgtcccta gcgttccgat cagcaccaga ggccttgagc cggcgtccgg
    19621 ctgcgccctg aacccgtgcg ccatacccgc aactgccaga gccgcgttgc gatacacatc
    19681 gcggctgatg gtattgagtc cggaaatatc gcagacggca ttgaccatcg tgatgtcccg
    19741 cgttcctacg aatggcgcgg taaagccgga tgcaagcgtc gagatcatga ccttgggcaa
    19801 accataggaa aagctgcgca tgacggcagt cgcgagcgtc gtgcccatcg atcccccgat
    19861 ggcaaaaatc cccgccaacg gcgcagcggc atcagccgcg tgcgccgcca cgatcgcgcc
    19921 cttggtcatt tccgcctggc acttgccttc atgttttagc gcacgaatga gttccaggct
    19981 gcttccagac gccgccgcaa ctgcactggg cggaatttcg gcgttgccga cggtgcggcg
    20041 gatgctggcg tcaaggtgca caacctcgac gccctgccct tccaaatgcc cgcgcaagaa
    20101 cgctgcctcc tcagccttgg tatcgagcgt cgcgagcagc aggacgcgag gtttggattt
    20161 tgacatggac attctctctc gaatggacag gggcacctcc ccactatcgt gtccatcata
    20221 cgatgaaaac gcgactggtg gcgcttgtta cgccctatgc cccacaattg gtgttggtcg
    20281 aacttactag gactgacggg ttcgtctttg gcctatcggc aacataattg gacaattcgg
    20341 caatagagat ttctggccta tctctagcag taattcttcg ccattcgtgc ttaaacgact
    20401 aatctcacga taatgcaaag agaaaaacgc atgtccggcc tccgagttaa gtcccgtcgt
    20461 cttgtcctgc tggctgtagc gctcgccctg ccagcacaag cccaaaccca tgacgccccc
    20521 cattacaaag tcgatccctt ctggcccggg caacttccca acaaatggat catgggccag
    20581 gtgggaggca tcgcggtgga cagccaggac catatctggg tcttgcagcg tccccgctcg
    20641 gctaccccgg acgagctagg cgcggcgcag aatcctccgc gaagcgactg ctgcatggcg
    20701 accccggcgg tgctggaatt cgatacagca ggtaacgtaa tcaattcctg gggcggcccg
    20761 aacttcgtga aagattggcc cacaaccgaa catgccatct gggtggacaa ggggggggac
    20821 gtctggatca gtggagccgg cgtcgatgat cgccaggttc tcaagtttac gcctgatggc
    20881 caccaaattc tggagatcgg gcgcaagtcg aaagaaccta aaaacaatca ggacacaacc
    20941 atgttgggca gggccgccgc catcgaagta gatgacgctg cgcatgaggt ctacatcgcc
    21001 gatggttacc tgaacaaccg catcatggtg tttgactcgg ataccggtaa gttcaaacga
    21061 ggctggggag gctatggaat gcccctgagt caggtctcaa attctgtcga acagatcgag
    21121 ggtgaagttc cggcagcagg ccaaattccg ccatacgcag ctagtgaccc gccggacgga
    21181 caattccgtt cgcccgtcca ttgcgttcgt ctctccaatg acggactggt ttatgtttgc
    21241 gaccggcata atgaccgaat ccaggtattc acgaaggcag gaaaattcgt ccgggaattc
    21301 ttcctgcatc ctgagacgtt ggggaatgga tcaacctgga caatgacatt ctcacacgat
    21361 gtcggccaaa aatacatgtt ggtggtcgat ggcgagaaca acacgatctg gatcgcaacc
    21421 cgaagtgacg gcagggtgat ttcacatctc ggtcatagcg ggcggaattc tgggcagttc
    21481 cactgggtgc atcaagccgc catggattcg atgggaaatc tttacacggg tgaggtcgac
    21541 accggcaagc gcgtgcagaa attcgtcctg gacagatagg cagcggcatt ccccggatga
    21601 ccccagattg acatggaaag tagcgcagtt ccggggcccg atgaagcccc tgaacagcgc
    21661 ccggggctgg tagggcggct tctatgcctg gctgaatagg cgccaaccag cgcagcccca
    21721 gcggcgaaga actgcaccga gtccagacgg gcacacggtt tgtgtcttcg ggccgccggc
    21781 gattaggttc cggcgaatcg gcgggctgtt acttgggcgg catcagaagg tgcacgctca
    21841 tgatgacaaa ctcgcccttc acgccctgga actggtgggc ggtgtcgggc ggcaccatgg
    21901 cgtaatcgcc ttcgacgatg tgccgcttgg tggcgcccac aatgccggtg ccgacatcag
    21961 aggcactgcc ggggccggaa gtcggcggca ggatgccggt caggtgcccg cccgtggcca
    22021 gatccgccga acctttcagg acatgcacca actcggcctg gccgtgatgc accgtcggcg
    22081 gcgtcacccc ggtgcggtat tccagcagca cgagataggg cgcgacgcta acgatcggca
    22141 ccacagaatt gatggtgggc gattcctggt tcgccttggc cttggcgatc agcgcgggaa
    22201 tatctgcgcc ggaccggaac agcccttcgg gcggggccgg ctgggcggcg gcgggctggg
    22261 ccatcaccgg taccgccatc accggcagga tgacggcaaa caaggcatgg gcgatcttca
    22321 tttttctctc cctgggcaaa aaagggccgg gttatcccgg ctcccgtccg gcgatccggc
    22381 tattgtttgg cgctggcctg gaccttgtaa ttgtccattc cggtcttgac tgcaagaccg
    22441 aaatgggggc accccgccga tctttcactt cagggcttcc gcggtcagaa acttccttca
    22501 tgtcgaaggg gggcaagtac gacctcgccc gccctctttt acgtcatatg gcccatcgag
    22561 cgctcagaat ttcgcggaga aacccaccga aaccacccgt ccgagcggat tggctactgt
    22621 ccggtcataa cctctgccgc cggctgtcga cccctggata aagggcggcg ctgtatcgaa
    22681 caggttctta gcatcgatat agatctggtc gcctcccaat atcccacccg cgaaatcata
    22741 ggctgcgtgc aggtcgagcg tagtcaaaga gttgacctta tccccgcctc catttgggtt
    22801 atgattcgca tcaaacgtca gcggattgac cgtcccaggt ccccacatac gatacgaccc
    22861 ggtgaaattc acgaaggcgt caattgaaaa ggcactgtca gtccaaccca gattgagacg
    22921 attgcgggtc tggaccgaag ggaagttggc gttatagcca gcggtattca agatgctgaa
    22981 tgaagtgaca ccctgaccgg cggcgtagtt atatcgcgta aactccgtca gagagtcccc
    23041 aaccgtgaac gtgcccatag cggtatcgaa tgaatactga ccattcagat cgatgccgcc
    23101 gatatccagg tacaagacgt tcgcctggct gaagtcgaac aggaagtacg cgaccgtcgg
    23161 catcgtgccc gacttgagag gcagtcctgc cgtcttcgcg tcgatctgcg cttgggtaca
    23221 tccggtcggg cagaccgtga acttgggctg cagcgcgggc gtggaaactt cggcgccgac
    23281 cgacggcgaa agaacaccgc ccttcaattt attattaaag tatgtgatct gcacgacaaa
    23341 gcccggcagg aacgagggtg caagatcggc gccgacggac cagctgtttc ctgtttgcgg
    23401 cttcaaagtc tttgcaccgc cacgatagaa ggtaatgccc ggtactgctg cggtgcccgc
    23461 ctggcatact gtagcagtcg cagcacagcc tggcagaacg ccagcaatac ccggaaacgc
    23521 agcggtcgga atattgaacg ccacaccatt gatggtcgca tagtcgccca cctgagtctg
    23581 ggcggcattg tagtactggc ttgggtcacc aaggctgtcg agcgaaggcg cgacgaaaga
    23641 cgtcgcataa gtcgatacca gcttgaaccc gtcaatgatc tgccagcttg ctgaggcctt
    23701 aggattggtg gtcccaccca catcagaata ttcgtcgtgg cgaccggaaa gatcgatgtc
    23761 gagtttctgc acaagcggaa cattcatact gggagatatc agaggaatcg caagttcggc
    23821 gtattcggac tgcacggtat gcgcgaatct gtattccgcg gccagcgagc ctttctggga
    23881 ggtcccggtg ccgttgggag cgatgacctg ctggatcagc gccgcatgga agttttccag
    23941 accagccgcg accttcacgg ggccggcggg taatgaaaac aagttgccat tcagaccggc
    24001 ggtaagctgg tcaaaattgt tcaaattgtt ggtgaagctg ttgccggtca ggctggcgat
    24061 caccgcggcg gaggtacgat tcgaagttcc ggggggattc cagacatcca gctcgttcga
    24121 tgcgttcagc ggtaggtttg tcgcatacac ctgagtaccc gcgatggacg gtgtagtcag
    24181 gctccctgag ccattggtgg tgccgttaag cgcaaggttg gcacaggaag cacagaaaag
    24241 attgtatacg ttactgaacg tatagtcctt tccgaggcta ttggtgacag tcagcacaaa
    24301 attgtcgtcg atattccact ccgctttcgc gttggcataa aaggtgttgg caccttcctc
    24361 gttcagcgcg agcggaacga acccggacgc agaccagtag actgtttcgc tggtagcggt
    24421 ggagccggga acgttcatat agaaggaatt ggcctggggg ccggtcgcaa ataccgtaga
    24481 cgttacagtt ccaggagcgt atgggctaaa cggctggccg cgcttgaagt cttggcgcac
    24541 gtcataaacg atatccgcac taagcgttac gcggccaaat tgctgggacc ccttaataag
    24601 aatattgtct cgctcttctt gaggcaagtc atcgccctgt ccggcataat cgcagggtgc
    24661 gtttgcgctg gtgttcgcca cgggagttgt tgaagtaaca gatggataaa ttaccgagct
    24721 gccggcgggc ttgatagcgg cgggcgagca gttgaagcta gcgttattca ggccgccaaa
    24781 cgcgacgaaa ttagagacgg cggtgaaagg ccgcgcggca ttcgtcaaat ggctctgaaa
    24841 agaatggctt ccggccgcca tgatccaacc ggagtcccag tcttttccaa caagatagct
    24901 tgcgttccat gtctggtagc cattggcata gcccgcctgg ccggtgagct ggacgccgga
    24961 atagtggctg cgggtgatga aattgaccac acccgccacc gcgtccgaac cgtaaattga
    25021 ggacgcgcca tcagccagaa cgtcaacgcg ctccagggcg atcaccggaa tgattcctgg
    25081 atcggtttgc gaatgctgct cgccgccctg gggcaagcgg cggccatcca tcaggaccag
    25141 cgtgctgttg ctagcgctgg ccgccagctg atgaatgttc ggcgcgtaat aattggcgta
    25201 gccttgcccc tggggggcgc cgccggagga agaaagcgcc ggaattttac tgagaagctc
    25261 aaccatgctg gcaggtgcgc tcgccttgat tgcggcttga tcaacagtga taacattgga
    25321 gccgataggc gctattccgc gaatgctcgt gccggtaaca gtaacggcct cgaccgattc
    25381 cgcgtcctgc gcggaggcgg cctggaaacc aaatgcggtt ggcacaacaa gcaccgcaat
    25441 caactgcgca acacgctgcc gtgaaactat acccatcttg ttgttcccca ttttcgttat
    25501 actttcccac gcgcggcggg cctaacgcaa aaatacctcg gcattttgca gcgctaccgc
    25561 caatcttaca gccgtgtcgc aagccgtctt tgacggatcg gaagctgagc aggattggtg
    25621 cgcattttcg aggttatctt tggagcggcc cgggtggccc ggataagcct ccccggtggc
    25681 ggatttcgga acgaccccgc cagccggtag gcgcgtcttt atcgaccgag ggggaccgca
    25741 taaatgtgaa agctgccggg gtccttgcct ttaaggtcgt cattgcgctg gaagtcgaac
    25801 ttggattcca aaacccaggc ggtattacct accggagtca cggcgactgg cagttcgaag
    25861 ccggacttga gaaccttgat tttcgccgta tcgcccattg gaagaacttc ttccaaccgg
    25921 ccttccttca ggccggcccc tgggcgtccc tggccctcga tcatcaagat gcgtccgtcg
    25981 gtagataggc gcatgccatc gggttgatac agaggcaggt cggtcgtcag catggtcccc
    26041 ttgcccgcgg tcccgtcctt gttgacgtcg accctgtaaa ggtgattgcc gacataggtg
    26101 ttggtgtaaa gcttcccgtc tctggcccat accagcccat ccagcgtggg atcggagggg
    26161 tcctgcgtgt accatggcat tagcgtggca gcgcccgacg ccaacttgag gatacggccc
    26221 ttgatcgtgt ccgaaatgta tatcgtgcgg ccttttattg cgatgtcgtt gcacaggccg
    26281 ccacccggca aatcatagct attcagcttt tttccggttt tgagcgcaaa gcttttcagc
    26341 gtcgcggtat ggtcgtccat cttattgtcg cacacccaga gaatgttggc tgccgcgtcc
    26401 ggcaagacgc cgaggacgaa atgaaggtcg ccttccttct gcgaaatcca aggctccgca
    26461 taagcctgca tcggcccggc gcgataaatt ttcccggtgc cgctgccgcc gatataaatc
    26521 gttccatcgg gcgtcgcgcc catgctctcc ggaaatactt tttggtcatc gatcacgatg
    26581 tctgttggcg cggccagccc tgacccgcac agatcggcgg cgatcaaaac gagcgcaaac
    26641 agcttgatgt acgccatgga ctttccctcg tttatcccat cctcggcatt atccgggcat
    26701 gggccgttac ttcggcgtca gctgaagcag ctttccattg tctgtcagga catagaccgc
    26761 gccctcgggc ccgatgcgga catcgcgaac gcgattgtgc atatccaata gaagcggctc
    26821 ttcgttcacg accttgtctc cggaaagcga taggcgatcc agcatcctgc cgcgcagaga
    26881 accaatgaag acactgcctt tccattgcgg aaacagattg ccgttataaa aggccatgcc
    26941 cgaaggcgca atcaccgggt cccagtaata tagcgactgt tccaaacctt ctttctcggt
    27001 aatgccctcc ccgattgttt cgccgggata atcaatacca tggacgatga ccggccagcc
    27061 atagtttgcg cctgctcgcg ggcgcagcag cttgtcatca cctcttgggc cattttcatt
    27121 ttcccacagc tgaccggtga ccgggtgtat cgccaggcct tcctcgctgc ggtggccgta
    27181 ggaccatatc tccggaagtg cccctgcctt cccgatgaac gggttgccgg cggcgggtgc
    27241 accatcgggc gtgatgtgga tgatttttcc cagatccgta tccagctttt gtgccatatc
    27301 ccagggcggc gaattggaac ggtctccgat tgtcacaaac agattgccgt cgcggccgat
    27361 cgcgatgcgc ccgccctggt tatccaccaa agtcttggcc ggtaatgccg gcctggcgcg
    27421 gaagatgact tcgacttttt cgagcgcgtc cgcggcctcg tcaaaaatgg cgcgtgcgac
    27481 gacaatattg ctgtcggtat cgccgaccaa ctcactgtag gtgaaaaaga tccgatgatt
    27541 cgcggcaaaa tcgcgatcca gcgccacatc cagaaggcct acttgcccaa aattgaatac
    27601 tgccggaacg ccggcgacag gcttggaaag ggtgccgttg ctgtcgagca ggcgcatgct
    27661 gccgggcttc tctgttatca ggacttttcc gtcgggaaga aatgcaaagc tccacggcga
    27721 cctgagcttc tctgtcagaa tcttgacgtt gaacggcgcg gtacgatgaa acggggcgcg
    27781 cgtctgaccg ggaaatgccg gtttatcgtc tgccttctcc ggaggacgcc tttcaatagg
    27841 ttgcccttcg accaggacgg cattgaagtc cgtgaggatc ttgtccggca ctaatggttt
    27901 cacgccgggc ggcgtacccg tcggcggggc tgtctgggcg atcactccgg ccggcaggat
    27961 agtcaggcaa acaacgcata cttcaagcaa tctcgacttc atcttattcc ttctcggagt
    28021 ggcgaccggt ctgccctcct gcagcttgtc catcggcaac aaacgtatca gtgcttgcca
    28081 agccgtcttt ggcaggacgg cagtggacta ggacaattcg gaaccaacag ccgctacccc
    28141 gatgcaatcg gcgggcatct tgcgattatc ccgcgcgcca ggccgctgcg ccgaacgtgc
    28201 caattctcgc cttggctgcg gttgccgtct tctggcatct gcgcggcacc aacgccgcga
    28261 ctctggttgc gccaaatacc acgccgcccg gatgggggtc tggtctgatt attttagtca
    28321 ggcagtccaa acacataaag catgttgccc gtctgggctc ggtgcacttc atccagcagc
    28381 gcggctggct tcagttgggg gctgccgccg cctaggccag tggtgacggc gatgaactgc
    28441 ttgccgtcca gactgaagct gacgggatag ccttgcacag tattgcccaa ccgggtctgc
    28501 cagatgatct tgccggtctt cacatccaca gccttgaagc tgcggctgaa gtcgcccacg
    28561 aaagccagcc ccccgcccgt cgacagcaca gccgatagga agggcgaacg ctgttgccag
    28621 gaccagactt ccttcatgtc gctggtgcga taggctgaca ggcggcccat attctggtga
    28681 gtgccgggca tgaagaaaaa cttctgtgac gccgcggtgc ccccgccgcc cagcttcatc
    28741 tccacctcat gtccctccat catcacacag ctctggctga gcgggatgat cagaagatcg
    28801 tcgggctggt catagctggt ggcctgccag tcatggccgc cctccggccc cggacacgat
    28861 gacagccact cattggtctt ctggttgagg atgtcggagc gataggtcag cgcgccggtc
    28921 ttgtagtcga tccccgagaa gacgttctga aagatcgtct gtttcacgcc caggaatttc
    28981 ccgctttcgc ggtccaactt ccacaatagt cccaccttgc cgattgtcat cagggtcttc
    29041 tgatcgccat ggtcgatcag cacgcgctcg aacacctcgt ccaggtccag gctttcgccg
    29101 ggagaatgct ggaaatacca cttcagcttg ccggtgtcgg gatcgagcgc cagcgtcgaa
    29161 gacgaataga gcgtcgccgc attgccggtc ttgcggctgg cacgcatcca aggtttggtc
    29221 tgggccacgc cccaataggt cgtgttcagt tcggggtcat aggtcccggc aatccaggta
    29281 tcgccgccac cgcgaagaag attgggcagg ccgttccagg tatcgccgcc gggctgtcct
    29341 tccagcgccg tggtgaagaa tttccagtcg cgcttgccgg tctccgcgtc gaaggcgctg
    29401 atgtagcaat tgttcttgga atagttgtcg caaccggtca ggccggtcag caccttgccg
    29461 cgcatcacca tcacgccgcc ggtgttggaa ttgttggggt tgcccagcgt ctgctcccac
    29521 acctgcttgc cggtgcgcgc gtcgagcgca tggatatggg catccgtggt cgcgacatag
    29581 accttgtcgt gatagaccgc caggctgcgg gtcccgccat aggcgatctt cgacaccggt
    29641 ccgattcggt tttcccatat cagttcgccg gtgcgcgcat ccagtgcctg cacggtgttg
    29701 gacgtgttgg acaggaacat caccccggca tggacgatgg gcgtcacctg gctggcacct
    29761 ccctcgttca tgttccaggc ccacttcaac gtcagcgact tgacgttgtc ggtggtgatc
    29821 tccttcagcg gactgaagct ccagccctga tagttgcgcc ggtacatcaa ccattcgccg
    29881 tccggcggat gggtcagcat cttgtcggta acatcgacat agttcttcac cgtgcccttg
    29941 actgtctggc caagctggaa cgccggcgcc atctggtcgg tgggctggtt cagcggcgcg
    30001 tcattggcgg tcttggcggc tcggggcgct ctgaccggcg gcttgccggt ggcgatgctg
    30061 aaaaccttga cgtcggtgct cttgttaaaa gacttcgatc ccgccggcgc gccattggcc
    30121 tgcagcagat aggccgtgac atcggtgtac tgcgtctcgc tgaggctgcc gggcacgccc
    30181 gccggcatgg atgtggcgat gaacttgtat aaatccttgg tcgaacgggc accaaaggag
    30241 ctgatgaacc cgtttccgcc aagcgcgggc gcgtcgccgc cgccggtgag gttggcgcgg
    30301 tggcagccgg cgcagttctg ggcatagact gtcccgcctg ccgccgcctg ggccgcagtg
    30361 taaacgccta caggcgcggt ctgcgcccaa gccagcgcac ttccaagacc gatcaacgct
    30421 gccgcgcccg tcacgaaact ccacgacatc atcattttcc ttccagccag ccctgcaggt
    30481 tgccctccgg cgccttcgcg ctgtcgatgc ggatataaag cgactgcttc tgcagtgccg
    30541 ccacctggtc ctgcgtcagg gccaccgatc ccgacacctg accttccggc gcatgtgcga
    30601 cggtcagctt gccgatcgct tcgcccggca cgcccttggc caatccgacg cgcaccgaag
    30661 cggcggttgc gggcgacgcc atgcgggaga agttgccctg gatcgtcagg atgttgccct
    30721 ccaggctggc ggtgatgttg ccgatgctgc cgatcacgtc ggcgctgttg gtggcgtcga
    30781 aaggcgcagg gtccagttcc gcctcgaata tctgagtgac ggccatagcg ggcgatataa
    30841 ataaaatcaa cacccctatg gcaaatcgat atatcttatg ttggcgtatc atggtgccaa
    30901 tgctccttgc tatttctcaa gcctgcttgc cgtctcatat gtgaggccgc tttcccgcgc
    30961 gtatgacata taggaatgcc ggaaaatgtt tcggagattc ggccgcccgc ccggcacgtc
    31021 agttcgacgc cagcttgacg cgatacatgg ccccggacgc aggcagcacc actgcgccgc
    31081 gtgccggcgt ggcacccggc agcgtctcat caggagtcca gacgccgccc atatagcgtc
    31141 cgcgttcgat cagagtgaag cgtccgctat cgcgacccga cgcggcaggc ggtcggaagt
    31201 cgacgacggc atcgaagcca aacacaagga attcgtccgt tgccaattgc gcaaccagta
    31261 cgcgccccga aggtggggcc ggtgttccct ccagatccat ccataccggt cgcttagact
    31321 tcgccggcgg gaagatggcc agcacgtccc aacgatcgaa cgacagctgg cggcccgcaa
    31381 tgttctccga ttcgatggcg gactgcagct tgcgcgtgcc gagtagcggg gtaatcaccg
    31441 gggcggcatt cgacagcagc ccatagtttg ctgccatttc gacgaagtcc ggtccccact
    31501 ggccatcgtc gctggcacca aacagcttga tcccggcgcc aaactcggcg cagcccatcg
    31561 ctccatactc accgatggcc atgaagcagt agcgagcgaa atcgagtccg cgtccggctt
    31621 cgaccaaaag cagcggattg tcacgacgcg cgtagcgatt cattatggtc cgattgatgc
    31681 tcagggaacg gttgtagatg tcgggcgcaa tgagatcgat ggcggaagcc gcggccttcc
    31741 accagtctag ggtatgcgat tgaccaccgc cgcttgggta cgtctcgccg ggacgatcaa
    31801 agtcatagta gcgatccccc gtgccggcgc cgccctgcca ggaatttacc agcatcggca
    31861 gtgggtaggc ctgcttgccg gccgcagcca cgctgtcgat gtagcgtgcc atgtagtagg
    31921 aagtgaaggc ttcctcgtcc acgccggcac cgaaggcttg ggtccaggat cccggcttga
    31981 ggtgtagcgc cccagcgaac gcccgcggca ccggcgcatc gaacagccgg ttcgccgccg
    32041 ccgagtgatc acgcgcacta ccgacgatgc cgggttcgtt ctcgacctgc accagtatca
    32101 cggtgtggtc aacaccgtcc acaatgcgca ggtgctgcat gagcgcggaa aatgccctac
    32161 gatccgcgcg cagggtggcc tcgccgtgtg gcgacagcga cttcgttgct tggccgttcg
    32221 cgtcgaccgc gagcggatag cgcacgtgat cgtcccggat ccagtcgggc aggaactgcg
    32281 actcgtcgcc cttccaggta ccgaaccaca gcacaatggc ccgcaggccc tggcctcgga
    32341 cgtcgcggat gatctgatca acggcaccga attcaaatcg ccctggctgc ggttcgatca
    32401 gcttccacgg aaccggaaat tcaaccgtat tggcatggtg ctgccggaag cccggcaaaa
    32461 ccttggccat ctcctgcggc gtgaaggtga tgttgtcgac ctgcgccccg agcatcagaa
    32521 acggctggcc gtcgaccagc agcgcgtgtc gtgcgccatg cgtgaccagc tccggcagcg
    32581 gacgcgcctg tgccggagac aacgtacagg ctgctgacag cagaatcagc gtgattatgc
    32641 atcttcgcac ggtgtcttcc tccaaagcga agaggctccc gtcgccggga gcctcttgcg
    32701 gtgcgcagca ccacggttgt tgcttcctcc ggatcagtcc tctgagccga gatgataggt
    32761 cacctgcgcg ccgaaggtca tgccgaaatc aagattcgga ccccaagtat cgcgcagcgt
    32821 ggcaggatcg ctggtcgtcg ccatgaagtt cgattggctc tgcagcagca cccgcgtgtt
    32881 ggtcaggttg cgaccgtaga ttgcgagctc ccatggacca ttctcgggag tgagggccac
    32941 gcgcgtgtcg atgcgccagt aagcgggctg gtggtcgagc ggatcctgct ggtcggagtc
    33001 gttgtagccg ctattacgga gcaggttcgc gtccaaagtg atgcgatagc cagactccac
    33061 tgagaactgg tagttgggat tgatgccgac ctgccacttg gagctgtacg ggagcgccac
    33121 gccattgcgg ctgacaccaa ccgcgcactg cgccggtatc agcttggcct cgagttggtt
    33181 gcaagacgcg ccgttgaact cgtcgtaact ggcgtcgcca aacaggttac cggtaaagcc
    33241 gatggtaaag tcctgatccg gtatcggcag ccagcggcca tcgaactcca gtccctgggt
    33301 gtgggacctg cccgcattgg tggtgatgaa cgcgtttgac ggtgcgtcat agacgctgac
    33361 ctgcaggttc ttgtacttcg tgtagtagta gtcgaccgcc aattggagac tgtcgcccag
    33421 caggttagct ttgatgccgc cctcaaatcc ctgtgccctt tcgggcttgt aggtgaacgg
    33481 attcggcacg ccgccaaaga atattggcga catgacgaaa ccgccagctt tggcggcgtt
    33541 ggaatatttc acataggcca tcacattgtc gttcatatcc cattgcaagc tggcttccgg
    33601 cagaaagtcg ccgttcttca agttgcccac cgcctgcgcg ccgccgggaa acggcagcat
    33661 atcgctgaac gccgccgcac cgggcagcaa gtatgcatag gttgccggca ggcggccttc
    33721 cttgctgaca ttctggtagc gaccaccgag attcagacgg aagtcctttg taacgttcca
    33781 ggtggcagca gcgaatgcgt tgtaccagtc ggcagactcg aacagtgtgc cgccaaagcc
    33841 agccgccgtg gcggtgggcg gcaggaacgg cgcaccgaaa ttggtggggg cgtagacgtt
    33901 gatggtagtc tcgagattat ggtgcaggta ggaggcgccg atcatccacg agaccgtctg
    33961 ggccaccggc gaggtcagcc ggaactcctg tgaggtctgc tggaacttct cggcacgatg
    34021 gtcgctgaac agagcaaacg cactatagtc cggatcgagg atgtaattct tgctgtacct
    34081 caagaacgac gacgtcgatg tcagggttag gtcgcccagc tgccagtcga agttacccat
    34141 caagacgtcg aaattgtagg tgcggttttg tggttgggcg aactctttta ccttgttcag
    34201 accatagggg atggcctgta agccggtaac gcccggcccc gaattgtacc cccaatagcc
    34261 cgggcttccg ggcgcggcgc cgctggccgc gttgagggcg tccactgccg cgcgtgcatc
    34321 caatgagccg ccctggtagg cctggtgacg gttgttcaga tctgcgccca gtaccgcgac
    34381 gttgagcgcg caggctgccg gcatgcccgg gtcgaggaac ccattgccac ggctgtactg
    34441 cggacgcatc tcgcagatgg aatagctgcc accctgcccg aagttgaagg tgctctggtg
    34501 ctcgtacttg aaggtgaagt cgaaggtgtc ggtcggattc caagtggtta tcaggcgcac
    34561 cgcccagtcc cgcttgttgc ccactgccga accgtcgtaa ttgtggtaat cggtattggg
    34621 caggtcgtag aagcgtcccg agagccgtat gccgaaggtg tcggtcacag gtccgccgat
    34681 gccggcattg accgatagtt cgttgaactt accgtaggac acgctcgcgt cgccgcccag
    34741 ggtcttgccc ggcttgcgat cataaatact gatcgcgccc gcagtggcgc tctggccggc
    34801 gtaagtcggc tgcgggccgc gcaccacttc catgcgctcc acgtcgaata cttcattctg
    34861 gtccagagtt gcgcgaccgt agtagacgcc gtcgacaaac tgcgcaaccg cctgctcgaa
    34921 cccttcattg tccacgccgg taccgatgcc gcgcatcagg agctgatcgc cggtgagatt
    34981 gtgacgtata aacaggttgg gcatagtttg cgacatggat tgaatgtcga cagagccctg
    35041 attcaggatt tctttgccgc tgagggcgac gatggaagaa ggaacgtcct gtatattctg
    35101 ctcgcgcttc tgggccgtga cgacaatctc ctccagcgtg cctttggcag tgtctttggc
    35161 agtgtccttc gtagtgcctt gctgcgccgt agtgccgttg gcagtatcct gctgcgccga
    35221 cgccggcagc ccagacaaca gcgcacaaga agccgctgct gccaacgtga acacgattct
    35281 atccgccttc atcgcaatac ccccaaaaat agtgtttccc caagcgatca cgtagcccca
    35341 tggcgtacgc gcgaagtgct agttcaatgc gaccaatcct agccgatgta tcacaagctc
    35401 tcttgtctca gtcggcagtg tagttgaacg atccggaagt ccggttggcc ggcagagccg
    35461 ggatgaagta accggggctt ccgcgacagc ggcacgggaa aggcatggac gctgccggag
    35521 ccactgaccg gctcttacat cgcccgcgtc agcgcttcgc cgacgctcca gtggcctggt
    35581 ttgtcaacac ctcgcacata ctcgaggaag aattgcatcc tggagcggaa aaggggtcca
    35641 ttttctgggc gaagtcacag ctatgaagaa ccagggtagc agaccttgct aagcatcttg
    35701 gacgagcggg caacgaagtt ggattattgg gaggttctga tggctttagc ttttttcgag
    35761 catgttctct gtatgtggtt tccgaattag ggtaaatatg ccccattggg gaaagccaca
    35821 aagacgaaac agcgtcgctc gccagaatta cttcccagat tgttgcaaaa actaaattga
    35881 tgaaccggag gggccgaatg acctcgcgtg cgcccgcacc agaaatatcc gtaccaggcg
    35941 agccagtctc agtgacgatc gctcagaatc agaaggaatt gcttgccagt ccagattccc
    36001 agctcgcagc gaacaagagg ttggtattcg attggtggcg cgaagtcctc gttgcagcac
    36061 atgtcgaagt cgccgataag tatatgcacg ccgattatat tcagcataat cccaatattg
    36121 ccacggggcg tcagggtttt gtgaatttct tcagcgctag accccgacaa gagatcagct
    36181 cgacgattgc caacctggta acgatcgtag cagagggcga cctcgtagtt ttttcattcc
    36241 ggcgggagct cccagatcca cgagattcca caaagcgata tacttcgaca tggttcgata
    36301 tgatgcgtgt aaaagacggc aaaatcgctg aacattggga ttatggtacg aaataacaat
    36361 gccgattcat cattggcggt ggccaagcaa ggaacgaact gctagggatt gcgtaccgca
    36421 aattccgcaa tcacgtcgtc cgcaaactta ggaggaagaa atgaatttct ttgccaaagc
    36481 gatttcgagt gttgccgtcg tcgcatgttt gggcgctgca gatttagctt ttgccgcacc
    36541 cgccgaaagc accagcgcca ctgcctcaga tccagaaggg gcgccgccag atccggggca
    36601 cgtcccattc gtgctaccca aagacattaa atggattgga actccgggaa agcaagaaac
    36661 cgccttcttg tatggcaatc ctgataaggc cggaccctat gctgtcctct ataaatggta
    36721 tcccggagcg ttctcccacc cccactttca tgaccgaaca cggtggatct acgtcgtctc
    36781 gggtacatgg tgggtcagtt cgagcaacac ctacgatgaa aagctcacct tcccgttcca
    36841 tgccggcacc ttctccacag atgtcgcaaa taccgtccac tgggatggta atcgtgcagg
    36901 cgaacaggag aatgcagtca tactgcttgc gggaattggt cctgtgaaga ccatccaagt
    36961 cgacgaacag ggaaggccgc agtgacctgc ttgccgcagg agcatttcct atgaacattg
    37021 ttgcgaggcc ggtatatcgg aacgccacct ttataggtgg cgccgaaagt tcgccaccga
    37081 gcttcctggt gaggtgaaga ataggatggt catcggaaag acttgatcct ccatgggatg
    37141 atactttcaa tgcaggttca tccacaggat cggtgatttc attttttggt tttggccgcg
    37201 atggattgca aaattacccg ggtgcgccga cttctgcatg atatctgtgg caatccggcg
    37261 gtccatgcac ggttcttaaa tacgctttcg ctccttgagc acgctgggag ccgcaagata
    37321 atgcttagcc aatcgcggcc cggcgcgtct ttggatcatg aaaccctcaa gcatcttgcc
    37381 gaagaaacac ggcatgccta cttctttagg cgggccgcgg aagctgtcgc gcgcaggaag
    37441 cttgattaca gcgagaatga tcttttggct ggcgcgcatg ctcgtttgta catggggcgg
    37501 ctcgacgctt tcattacccg gtccgtacag ggccctcttg cttatcttta tatgacgctc
    37561 gtcgtcgagc tgcgggccac atggttttat aacctctacc aggatgttct caaagaactg
    37621 gaacagccga ttaacttaaa gggactgctc gccgaggaga aacggcacct cgaaggcatc
    37681 tccatgcaac tttccgctct aggagaagac atcaacgtct ccctaccgct ccttaaatca
    37741 tttgaagagt cgaaatacgc ggccctgctg ggggcgctgg agcgctcggc caacatttag
    37801 cgacgcatgc gccatcaagc gccggaaatg tcgggacaat tccccgcatg tgcggaaagt
    37861 atttatggag atataccaat cagat
//
LOCUS       DASZYN010000467         3241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_472428, whole genome shotgun sequence.
ACCESSION   DASZYN010000467 DASZYN010000000
VERSION     DASZYN010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472428"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VHE33_12110"
     CDS             <1..355
                     /locus_tag="VHE33_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98242.1"
                     /translation="FHAGDKTVEGVEYFDPQGPDHIWYRFNVSGEKGLILGSYSFAAS
                     DPDQEAYHLQHPDAVGSSLHRFVLQSSFSGTASTLGTIDGAPTYDDFRTRIVSIVQAE
                     TVTASASDTGTNAPH"
     gene            445..990
                     /locus_tag="VHE33_12115"
     CDS             445..990
                     /locus_tag="VHE33_12115"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HVT98243.1"
                     /translation="MEPNEARELQEQHEEAAANRRMHPVSFTMSVLAVMVAIVTVLGH
                     RTHTEAVLSQARATDEWNLYQAHKIRQYDTQLITDLLSTLPIRDQAAANKITDSYKSH
                     VQKWNEDLTETQNRAKEMQGEVLRSERRADRFDLAEALLEIGLVVTSITLLAGQRAYW
                     YMGLCFGIAGVITAALAYAVH"
     gene            1070..2104
                     /locus_tag="VHE33_12120"
     CDS             1070..2104
                     /locus_tag="VHE33_12120"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVT98244.1"
                     /translation="MKFAQYVVAVLALLVAPGSLLRAQKAGDLKIFFIDVEGGQSTLF
                     VTPDQHSLLIDTGWPGHEGRDADRIAAAAKKAGLTRIDFVLLTHYHVDHTGGVPQLVE
                     RIPVGTFIDHGPLREPGDKATSNAYEAYQKVLASGKYKHILAKPGMMLPIPGLTAEVL
                     TADGDVLQKPLRGAGQPNPFCEKSEHRAADQTENSRSVGTLLTWGKVRILDLGDLTWD
                     KEMDFLCPNNRIGHVDILVVSHHGWYQSSSPALVDAITPRIAVMDNGAKKGGSKPTVE
                     TLRRIPGLEALWQLHSSEEAGTDNAPEKFIANLPGSDTGYGFELNVSRNGSFTVTNAR
                     TQATTRYAAH"
     gene            2322..>3241
                     /locus_tag="VHE33_12125"
     CDS             2322..>3241
                     /locus_tag="VHE33_12125"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HVT98245.1"
                     /translation="MPGQQEVRWSQLKVGVLVIVALCALVALVFLMSGSTGGFWSGHL
                     ILRSYFENSAGLKVGAPVTLEGVTVGNVSKIRIRPERAPTPVEVEMEINKHFQDAIRQ
                     GSKTSLTTQGVLGDTLVDIDMKYAKGGPVQNHSELPTTESPNLQDVIQASQGTIQRLD
                     TILDQVNQIATTLNSKNGSIGLLINDPTLYKKAVHTVDELTSIVDQINNGQGSIGKLI
                     QDDSMYNRLNDMVAKADDITTKLDQGQGTAGKLLKDESLYNELKQTTHNLNEITASIN
                     SGKGTLGMLTKDPKTAAKLSDTIEKLDTMLT"
BASE COUNT          688 a         1009 c          963 g          581 t
ORIGIN      
        1 ctttcatgcc ggcgacaaga ccgtcgaggg ggtggagtac ttcgatccgc agggcccgga
       61 tcacatctgg tatcgcttca acgtctccgg ggagaagggc ctgatccttg ggtcctactc
      121 gttcgcggcc tcggacccgg accaggaagc ctaccacctg cagcaccctg acgctgtggg
      181 gtcgagtctg catcgatttg tgctgcaaag ctctttctcc ggaaccgcat cgacactcgg
      241 cacgatcgac ggcgcgccta cctacgacga tttccggacg cgcatcgtga gcatcgtgca
      301 ggcggagacg gtgacggcga gcgcgagcga taccgggacg aacgccccgc actaaacagc
      361 gatccgaatc ctccatcgcc cccggctccg ttcattcagg tttgcgtgcg cactctgcgc
      421 tcagcgccct gtaccctgtt gttcatggaa ccgaacgaag cccgcgaact ccaggaacaa
      481 cacgaagaag cggctgcgaa tcgcaggatg catccggtca gcttcacgat gtccgtactc
      541 gctgtcatgg tcgcgatcgt taccgtgctt gggcatcgca cccataccga ggcggtgctc
      601 agccaggcgc gcgcgaccga cgaatggaac ctctaccagg cgcacaaaat acgccagtac
      661 gatacgcagc tgatcacgga tcttctctcc accttgccga ttcgcgacca ggccgcggcc
      721 aacaagatca ccgacagcta caaaagccat gtgcagaagt ggaatgagga cctgacagaa
      781 acgcagaaca gagcgaagga gatgcaaggc gaggtcctgc gctcggaacg ccgcgccgat
      841 cggttcgatc tggctgaggc gctgcttgaa atcgggttgg tcgtgacttc cattacgctg
      901 ctcgccggcc aacgtgccta ctggtatatg ggcctttgtt tcggcatcgc cggagtgatc
      961 acagccgcgc tggcgtacgc ggtccactaa acattcctgc gcgagcggcg cgatgccgct
     1021 cccatctatc atgtcccggt cgaaaaatta tcttttccgc ggagtctcca tgaagtttgc
     1081 gcaatacgtg gtcgctgttc tcgccttgct ggttgcaccc ggctctctgc tgcgcgcaca
     1141 aaaggccggc gatctcaaaa tcttcttcat cgatgtcgag ggcggccagt ccacgctgtt
     1201 cgtcacgccg gaccagcact cgcttctcat cgatacgggc tggccgggtc atgagggccg
     1261 tgacgcggac cgcatcgcag ccgcggcgaa aaaagccggc ctcacgcgca tcgacttcgt
     1321 cctgctgacg cattaccacg tcgatcacac cggcggtgtg ccgcagctgg tcgagcgcat
     1381 cccggtgggc acgttcatcg atcacgggcc gctgcgcgag ccgggtgaca aagctacttc
     1441 gaatgcgtat gaggcctacc agaaggttct tgccagcgga aagtacaagc acattctggc
     1501 gaagcctggc atgatgctcc ccatcccggg cctgacggct gaggtcctga ccgccgacgg
     1561 cgatgttctg cagaaaccat tgcggggcgc cggccagccc aacccgttct gtgaaaaatc
     1621 cgagcatcgc gccgccgatc aaaccgagaa ttcgcgttcg gtgggcacgc tgctgacctg
     1681 ggggaaggtc cgcattttgg atctcggcga cctgacctgg gacaaggaga tggactttct
     1741 ctgccccaac aaccggatcg gccacgtgga catcctggtt gtgtcgcatc acggctggta
     1801 ccaaagctcc agcccggccc tggtcgatgc catcaccccc cggatcgccg tcatggacaa
     1861 cggcgccaag aaaggcggct ccaagccgac cgtggaaacc ctgcgcagga ttcccgggct
     1921 ggaggcactg tggcagctcc attcctccga ggaggccggg accgacaacg cgccggagaa
     1981 gttcattgcc aatctccccg gctccgacac cggttacggt ttcgaactga acgtttcgcg
     2041 gaacgggagc ttcacggtga ccaacgcccg cacgcaggcc accacccgct acgcggctca
     2101 ctaagcagaa gagcggcccg cctatcgctg gggcctgaaa acggggcgtc aagcgccggc
     2161 atgaggcaga tcgggctgag ttctgcaacg gatgttgtgc ccgtgtcatc agaccgtcat
     2221 gggcccgcag aggagcccga agtgcgttcg cctcaacaag agagtcctga tatcctctgc
     2281 gcaacggctc ttcttgcacg ctttgtctcc cgaggaacta cgtgcccggt caacaggaag
     2341 tgcgatggtc acaactgaaa gtcggcgtcc tggtgatcgt cgcgctgtgc gccctcgtcg
     2401 ccctggtgtt cctgatgtcg ggcagcacgg gcggcttctg gtcgggacat ctgatcctgc
     2461 gctcgtattt tgagaactcc gccggcctga aggtgggagc cccggtgacg ctggaaggcg
     2521 tgacggtggg caacgtttcc aaaatccgca tccgcccgga gcgcgcgccg actccggttg
     2581 aagtggagat ggagatcaat aagcatttcc aggacgcgat tcgccagggt tcaaaaacca
     2641 gcctgaccac gcagggcgtt ctcggggaca cgctggttga catcgatatg aagtatgcga
     2701 aggggggacc ggtacagaac cacagcgaac tgccgaccac cgaatcgccg aacctccagg
     2761 atgtgattca ggccagccag ggtacgatcc agcggctcga tacgatcctc gatcaggtga
     2821 atcagatcgc gacgacgctg aactcgaaga acggttcgat tgggcttctg atcaacgatc
     2881 ccacgctcta taagaaggca gtccacacgg tcgatgagct gacttcgatc gtcgatcaga
     2941 tcaacaacgg gcaggggtcg atcggcaagc tcatccagga cgattccatg tacaaccgcc
     3001 tgaacgacat ggtggccaag gccgatgaca tcacgacgaa gctggatcag ggacagggca
     3061 cggcgggcaa gcttctgaag gatgagagcc tctacaacga gctgaagcag actacccaca
     3121 acctgaatga gatcaccgca tcgatcaact caggcaaagg cacgctgggc atgctgacca
     3181 aggatccgaa gacggcggcc aaattgtcgg acaccattga aaagctcgac accatgctca
     3241 c
//
LOCUS       DASZYN010000468         3120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_275212, whole genome shotgun sequence.
ACCESSION   DASZYN010000468 DASZYN010000000
VERSION     DASZYN010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275212"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="VHE33_12130"
     CDS             complement(<1..1130)
                     /locus_tag="VHE33_12130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HVT98246.1"
                     /translation="MRRVAVWILIAATGGVACPAQSREDAARPLTVELFTSHRINVVT
                     ITPLGQNETAKLCTECRAKKVRQPLTVRLQGDAVDLGRNTSARELELEGAYRLTPDGG
                     VRPIAAAGVWKIAVAHGALRLLLTMDSERYVALALRGEAGAKEPIESLKAMAIAVRTF
                     ALENASRHRGDGFDLCDSTHCQALKFGTPSELVQRAVRETAGETLWYGSRRALVFYTQ
                     NCGGRSEDASQAWPGTRAQYLKARADPYCTRHGAAQWHAEIAVADLERVAQSAGWKLP
                     QRIRAVHVVKRTREGRVLRLGFLGKDGEVPVSASSLRFALNRALGWNQLRSDWYSVQL
                     REGVLSFDGRGYGHGVGLCQAGATEMATEGRSAADILQFYFPG"
     gene            complement(1127..1999)
                     /locus_tag="VHE33_12135"
     CDS             complement(1127..1999)
                     /locus_tag="VHE33_12135"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HVT98247.1"
                     /translation="MATIAIAVAIGCGDLSAWAAAGSNTVQRALDKALLGTNAVGVVL
                     DGADGRLLGVVRTSEAGRGASAPGSTLKPLFLAAALHAGRVRPETTVACHGNLKIAGR
                     NLACTHPREQNTFDAERALAYSCNTWFANLARRFSPREAVDVLHAAGFGSRTGLIAAE
                     AAGNVEVPGNDAEVQLLVLGLSGIRVTPVQLAQAYLWLAQRVDATPVVRRGLEGSVAY
                     GMAHNAATTGITIAGKTGTASDVGAAWTHGWFAGIASRSRERVVVVIYVPRGNGADAA
                     GLAHQFFALWGREQ"
     gene            2256..>3120
                     /locus_tag="VHE33_12140"
     CDS             2256..>3120
                     /locus_tag="VHE33_12140"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVT98248.1"
                     /translation="MSGFVPESIQRAYAIEPEPGSTYLDAEHVVILMQENRSFDHTLG
                     TLRGVRGFNDPRAIRLPNGNSVFIQTSAAGDSYAPWRLDIRDTRITWMGSIPHSRNSQ
                     VDAWNEGRHDGWIEAKRSGNHEYSNVPMTMGHYTREDIPFYYALADAFTVCDQHYCAV
                     MTSTTPNRSTFWTGTVRDRQTTDARVYMRNEQYERLQMPWKTYPERLQEAGISWKFYQ
                     NELTYSGLTGDHDAWLGNFGCNVLEFFGAYNIEAYSGFPLALQAESSGLTDEIARLEE
                     QAAKEKDPNIAS"
BASE COUNT          617 a         1070 c          825 g          608 t
ORIGIN      
        1 ccaggaaagt agaactggag gatatcggcc gcagaacgtc cttctgttgc catctccgtg
       61 gcgcctgcct ggcacaatcc cacgccatgc ccatagccgc ggccgtcgaa actcaaaact
      121 ccttcccgca actggaccga ataccagtcg ctccgcagct gattccatcc cagggcgcga
      181 ttcagagcaa agcgcaaact gcttgccgat accggcacct cgccatcttt ccccagaaaa
      241 cccaatcgca acacgcgtcc ttcccttgtg cgcttcacaa cgtgaacagc cctgatcctc
      301 tgcggcagct tccaccccgc gctctgtgcc acgcgctcca gatcggccac cgcaatttct
      361 gcatgccatt gcgccgcgcc gtgtcgagtg caatacggat cggcacgggc tttcagatat
      421 tgcgcgcgcg ttcccggcca cgcctgactc gcatcttcgc ttcgtccacc gcagttctgc
      481 gtgtagaaaa ccagcgcccg ccgcgacccg taccataacg tttcgccggc ggtctcgcgc
      541 accgcgcgct gcaccagttc cgaaggcgtc ccgaacttca gtgcctggca gtgtgtgctg
      601 tcgcagagat cgaacccgtc tcctcgatgc cgggatgcgt tttccagggc gaaggtgcgc
      661 accgcaatcg ccatggcctt gagggactcg atgggctcct ttgctcccgc ctctccccgg
      721 agcgccaggg cgacataacg ctcgctatcc attgtgagga gcaggcgcag agccccgtgg
      781 gcaacggcga tcttccacac acccgcggcg gcgatcggcc tgactcctcc atccggcgtc
      841 agccgataag ctccctccag ctccagctcg cgggcgcttg tgtttcttcc caaatcgacc
      901 gcatcaccct gcaatctcac ggtcagcggc tggcgcacct tcttcgcgcg acattcggta
      961 cacagcttcg cggtttcgtt ctgcccgagc ggcgtgatcg tcacaacatt gatcctgtgc
     1021 gacgtaaaca attcgacggt cagaggccga gccgcgtcct cccgcgactg cgcaggacaa
     1081 gccacacctc cagtcgccgc gatcaggatc caaacggcaa cccttctcat tgttctcgtc
     1141 cccacagagc gaagaactga tgcgccaatc ccgcagcatc cgcaccgttt ccacgcggaa
     1201 catagatcac cacgacgacc cgctccctgc ttcgcgatgc gattccggca aaccatccgt
     1261 gcgtccacgc cgcccccaca tcgctcgcag tcccagtttt gcctgcaatc gttatcccag
     1321 tcgtcgccgc gttgtgcgcc attccataag caacggatcc ctcgagccct cggcgcacga
     1381 ccggtgtcgc atcgaccctt tgtgcaagcc acaaataggc ttgcgccagt tgtaccggag
     1441 tcaccctgat accactgagg cccaggacca gcaactgcac ttctgcgtca ttgcctggaa
     1501 cctcaacatt gcctgcggct tcggcagcga tcaagcccgt ccggctcccg aaaccggcag
     1561 cgtgcagcac gtccacggct tcacgcggcg aaaagcgccg cgccagattc gcaaaccagg
     1621 tgttgcacga gtacgcgagc gcgcgttctg catcgaacgt gttctgctcc cgcggatgtg
     1681 tgcacgccag attgcgtccg gcaattttca agttgccatg gcacgcaacc gtcgtctccg
     1741 gccgtacgcg tcctgcgtgc agcgcggctg ccagaaacag tggtttcagt gtcgatcccg
     1801 gcgcgctcgc tccacgtcct gcctcggacg tgcgcaccac cccaagcaga cggccgtcgg
     1861 cgccatccag caccacgcca accgcattgg ttccgagcag tgccttatcc agcgcccgtt
     1921 gcaccgtatt cgatccggct gccgcccagg cagagagatc accgcagcca atcgccacag
     1981 cgattgcgat cgtcgccatc cagagcatgc gttccactct actcgcagcg gtctcctcgc
     2041 ccgaagcccg gtcgtcatcg gccaccgccg cgaatctgca tgaaatcgcg gggagcattt
     2101 tgcgttcagc aaacttttgc gggcaactct ttatttggat tgctcccgcg tgctgttgat
     2161 ttgcgaaata tctctttgcc acttcacaat atttcaatat gcagacgcga cgcgacttcc
     2221 tcaaaatagc tgcgatgttg tcaggggctt cggccgtgtc gggattcgtt cccgaatcga
     2281 ttcagcgggc ctacgcgatt gagcccgaac ctgggtcgac ctatctcgat gccgagcacg
     2341 tcgtaattct gatgcaggag aatcgctctt tcgaccatac actcggaact ctgcgcggcg
     2401 ttcgcggctt taacgatcct cgcgcgatcc gtctgcccaa cggcaattct gtctttatcc
     2461 agacgagcgc cgccggcgac tcctacgctc cctggcggct ggatatacgg gatacacgta
     2521 ttacctggat gggctccatt cctcactcgc gcaacagcca ggtggatgcc tggaacgaag
     2581 gccgtcacga cggctggatc gaggcgaaac gctccggcaa ccacgagtat tccaacgtgc
     2641 ccatgaccat gggccactac acgcgggaag acattccctt ctactacgca ctcgccgatg
     2701 cattcactgt gtgcgaccaa cattactgtg cggtcatgac cagcacaacg ccgaaccgca
     2761 gcacgttctg gacggggacc gtccgtgacc gccagacgac cgacgccagg gtctacatgc
     2821 ggaacgaaca gtatgagcgg ttgcagatgc cgtggaagac ctatccggag aggctgcagg
     2881 aggcgggcat cagctggaag ttctatcaaa acgaactgac ctattcaggc ctgacaggcg
     2941 accacgacgc atggctggga aatttcggct gcaatgtcct ggaattcttt ggcgcgtaca
     3001 atatcgaggc ttactccgga tttcccctgg ccttacaggc agaaagttcc ggtctgaccg
     3061 atgaaatcgc aaggctggag gaacaagctg cgaaggagaa ggatccgaac atcgcctcca
//
LOCUS       DASZYN010000469         2353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1046127, whole genome shotgun sequence.
ACCESSION   DASZYN010000469 DASZYN010000000
VERSION     DASZYN010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046127"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VHE33_12145"
     CDS             <1..205
                     /locus_tag="VHE33_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98249.1"
                     /translation="GRMAAFCGCCGAEITAKDEVCRICGTPRHGMLRADRMTPLDLGD
                     EVTLLDDAAGTRCCCPDSTVEEM"
     gene            478..1395
                     /locus_tag="VHE33_12150"
     CDS             478..1395
                     /locus_tag="VHE33_12150"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HVT98250.1"
                     /translation="MNRRNFLKGTGLAAAGCALGNAQARAPKMGPRVEFSTGSFTFAF
                     FTDVHIEPEMDAPQGTELAMEVINASDAEFAICGGDHVFDALKAHRDRILYQYELYAE
                     AEKTLRMPVWHVLGNHDVAGLETGMSHHDPIFGKALFQKVFNTPTYYSFQHKGVNFIM
                     LDSIMIKGHDWQPAIDIPQAAWLERVLQATRGTPSIVISHVPLATSMASYGPGSNNKI
                     YQPVLNANYIIPMLEYYDVIAVLQGHTHISETVHRMGIHYVTGGAVCGNWWKGAQYGD
                     REGVTFVTVDNGSITTNYVPTRFLTLEQS"
     gene            complement(1493..1819)
                     /gene="rpsT"
                     /locus_tag="VHE33_12155"
     CDS             complement(1493..1819)
                     /gene="rpsT"
                     /locus_tag="VHE33_12155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HVT98251.1"
                     /translation="MANHVSALKRTRQTTKKTAVNRANKTHFRGTLRALREALQKGDA
                     GTVNTEFRKTVSIIDKSVQKGVIHANTASRYKSRLNARVKAVATGNSASAAKTSAPKK
                     SAKSAR"
BASE COUNT          474 a          631 c          743 g          505 t
ORIGIN      
        1 agggcgaatg gcggcatttt gcggatgttg tggcgctgaa attacggcga aggatgaggt
       61 gtgccgcatc tgcgggacgc ctcgccacgg tatgctgcgc gcagatcgaa tgacgcccct
      121 ggatctcggc gacgaagtga ccctcctgga cgatgcggcc gggacacgct gctgctgtcc
      181 cgactcgact gtggaagaaa tgtaacggcc gggcggacgc cggcaggtgg ccggaagagg
      241 tctttggttc ttttttcttt ccgaagccgg gtcgcctcgt ataccctcaa tccgcccccg
      301 acaatcgatc gaacccccgt cgatcacttc tgaacgcttc gcttgcgcct gtgtgatgcg
      361 tgtcacaggt gcctttgcgc agcggcagtt agtttcaaca gtactctccc ccgattacca
      421 ctgcggaata gcgagtggac tgatgcagga cctaggctca gcagtcagga tgacgagatg
      481 aatcggcgga attttctgaa gggtacagga ctggctgcgg ctgggtgcgc gcttggaaac
      541 gcgcaggcgc gcgcgccaaa gatggggccg cgggtagagt tttcgaccgg cagctttaca
      601 tttgcgttct tcaccgacgt gcacatcgag cccgagatgg atgctccgca gggcacggaa
      661 ctggcgatgg aggtgatcaa tgcctcggat gcggagttcg cgatttgcgg cggcgaccat
      721 gtcttcgacg cgctgaaggc gcacagggat cggattcttt atcaatacga gctctatgcg
      781 gaggcggaga aaacgcttcg catgccggta tggcatgtgc tgggaaatca cgacgtggcg
      841 ggacttgaaa ccgggatgag ccatcacgat ccgatttttg gcaaggctct tttccagaag
      901 gtgttcaaca ccccgactta ctactcgttc cagcacaagg gcgtgaattt catcatgctg
      961 gattcgatca tgatcaaagg gcatgactgg cagccggcga tcgacatccc tcaggctgcg
     1021 tggctggagc gggtgctgca ggcgacccgc ggcacgccct caatcgtgat cagccacgtg
     1081 ccgctggcca ccagcatggc gagctacggg ccgggatcga acaacaagat ctaccagccg
     1141 gtgctgaatg ccaactacat cattccgatg ctggagtact acgacgtgat cgccgtgctg
     1201 caggggcaca cgcatatatc cgaaaccgtg caccgcatgg gaattcacta cgtgaccggc
     1261 ggcgcggtgt gcggcaactg gtggaagggc gcacagtatg gcgaccgcga aggcgtcacg
     1321 ttcgtgacgg tggacaatgg gagcatcacg acgaactacg tgccgacgcg gttcctgacg
     1381 ctcgaacaga gctaagcgga aaggtaaaac ggaaagagac gattgaggat cccagtgagc
     1441 agggagctgc ccgcaggctc aatctttccg aacttccccg agggctttct gactagcggg
     1501 cggacttcgc ggacttcttt ggcgccgagg ttttggcggc ggaggccgag tttcccgttg
     1561 cgacggcttt cacacgagca ttcagtctgc tcttgtagcg cgatgcagtg ttggcgtgga
     1621 taacgccctt ctggacgctc ttgtcgataa tggagaccgt cttgcggaac tcggtgttga
     1681 ccgtgccggc atcgcctttc tgaagggcct cgcgaagggc gcgcagcgtg ccgcggaagt
     1741 gagtcttatt ggcgcggttg accgcagtct ttttcgtggt ttgacgggtg cgcttgagcg
     1801 cagaaacgtg gttcgccatg agtgaatcga atctcgcttt cagaatggat gcaggcggaa
     1861 gagacgcgtg ggcgcattcg ccgcaaagag ttagtttacg gggttcggtg aggtgggtca
     1921 accgttggtg gggtaggggg atagcggccg ggaaaagtgc acccctttgc ccgtgctccg
     1981 catcgggtgc acgccgcgtc taaaaattca aaggaaccat cagtgtttga aactcccacg
     2041 gttcggattt gttgaccatt ttcctcgcct ctcgcggaac gttcttgcta gaatcggtct
     2101 cctccattta atcgctgaag tcaattgggg taccgcgcaa cagcaccaaa acttgctgtt
     2161 ttgccttcac cccatggcgc tctgcaatgc cgtattcttc tgacgttttt gacaaactga
     2221 taggcggtct tcttgaacgc tggaattacc gccgctacct agatgtcgga tgtggggcgg
     2281 gcaaatatgg gcgtatgatc cgtgcgctcg tcccggacag cacgatcgac gcgatcgaag
     2341 ttgacaccga ata
//
LOCUS       DASZYN010000470         2530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_789111, whole genome shotgun sequence.
ACCESSION   DASZYN010000470 DASZYN010000000
VERSION     DASZYN010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789111"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VHE33_12160"
     CDS             <1..482
                     /locus_tag="VHE33_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98252.1"
                     /translation="HPELAEHLAPVRGLISEDQIAAARANWNDACDICHKHCTGRVKE
                     IQRVAKVHRDPFEPIMPILEAESPVGEYRKIADEIIRTIPDELRYPSAAAEAVRSFLL
                     IRFGLHTGLRQKNLRQLLICPRGKLPTSERQLADMKVGELRWSERDHGWEVFIPSVC"
     gene            590..2119
                     /gene="istA"
                     /locus_tag="VHE33_12165"
     CDS             590..2119
                     /gene="istA"
                     /locus_tag="VHE33_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HVT98253.1"
                     /translation="MALLSVIRRWHLRDGHSIREIARRTGLSRNTIRKYLRSDEVEPR
                     FQVPERPSKLDAYAERLSAWLRTEANRPRKQKRTIKQLHAELMSLGYEGSYGRVAAFA
                     RAWKEERQRQMQTSGRGTFVPLAFEPGEAFQFDWSEDWAVIGGERTKLQVAHFKLSYS
                     RAFIVRAYPLQTHEMLFDAHNHAFRVLGGVPRRGIYDNMRTAVDRIGRGKERQVNARF
                     SAMASHYLFEAEFCNPASGWEKGQVEKNVQDARHRLWQPMPRFPSLAALNDWLEQRCR
                     ELWQQIPHGLRPGTIGDIRVEEARFLMPMPRLFDGFVEYTKRVSPTCLVHLERNRYSV
                     PASFANRPVSLRVYPERIVVAAEGQIVCEHCRIIERSHDGPGRTVYDWRHYLAVIQRK
                     PGALRNGAPFTELPEAFKRLQQHLLRKPGGDREMVEILSLVLQHDEQIVLTAVEMALQ
                     AGVPTKVHILNLLHRLVDGKPIETPIVDAPQALSLAKEPQANVERYDALRQTREVRHA
                     S"
     gene            2109..>2530
                     /locus_tag="VHE33_12170"
     CDS             2109..>2530
                     /locus_tag="VHE33_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011255145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT98254.1"
                     /translation="MRHDPASGAIVIMLRSLKMHGMAQAVAEFTEQASPAFEAAIPIL
                     SQLLKAEMAEREVRSVAYQLKAARFPVYRDLAGFDFASSEINEALVRQLHRCKFMDDA
                     NNVVLVGGPGTGKTHIATALGVQAVEHHRKRVRFFSTV"
BASE COUNT          482 a          806 c          794 g          448 t
ORIGIN      
        1 agcacccgga gctcgccgag cacctcgccc cagttcgtgg tcttatttct gaggatcaga
       61 ttgccgcggc ccgcgccaac tggaacgacg cctgcgatat ctgccacaag cactgcacgg
      121 gccgcgtgaa agaaatccag cgggtggcca aggtgcatcg cgacccgttc gagccaatta
      181 tgcccatcct tgaggcggaa agcccggtcg gcgagtaccg aaagatcgcc gatgagatca
      241 ttcgtacgat tccggacgag ctacgctatc cctcggctgc agccgaggca gtgcgttcat
      301 tccttctcat ccggttcggt ctccacactg ggctgcggca gaagaacctc aggcaactgc
      361 tcatttgtcc gcgaggaaag ctgccgacct ccgagcggca gttggcggac atgaaggtcg
      421 gggagctacg ctggagcgaa cgagatcacg gttgggaggt gttcatacca tcggtctgtt
      481 gatttccgcc gagaactgac ccgggattgg gcggtttttc cattgagaag tgacccatgt
      541 ttgaacccac ccctgctggc tgatcgcagg ggacattgga gtgatagaca tggcgttact
      601 gagcgttatc cgacgctggc atctgcggga tggacactcg atccgggaga ttgcgcggcg
      661 cacgggactg tcgcggaaca cgatccgcaa ataccttcga tctgacgagg tggagccccg
      721 cttccaggtg ccggagcggc cgagcaagct tgatgcctat gccgagcggc tgtcggcgtg
      781 gctgaggacg gaagcgaaca ggccgcgcaa gcagaagcgc acgatcaagc agttgcatgc
      841 ggaactgatg agcctcggct atgagggctc atatggtcgt gtcgccgcct ttgcgcgggc
      901 ctggaaggaa gagcgccaac gtcagatgca aacgagcggg cgcggcacct tcgtgccgtt
      961 ggcgttcgag cccggcgaag cgttccagtt cgactggtcg gaagactggg cggtcatcgg
     1021 cggcgagcgc accaagcttc aggtcgcgca cttcaagctc agctacagcc gtgccttcat
     1081 cgtgcgggcc tatccgctgc agacgcacga gatgctgttc gacgcccaca accacgcctt
     1141 ccgggttctg ggcggcgttc cccggcgcgg gatctacgac aacatgcgca cggcggtcga
     1201 caggatcggc cgcggcaagg agcgtcaggt caatgcccgc ttctcggcca tggccagcca
     1261 ctatctgttc gaggccgagt tctgcaatcc ggcctcgggc tgggagaagg gtcaggtcga
     1321 gaagaacgtc caggacgccc gccaccggct atggcaaccc atgccacgct ttccatcgct
     1381 ggcggctctg aacgactggc tggagcagcg atgtcgggag ctatggcaac agattcccca
     1441 tggcctcagg ccgggaacga tcggagacat ccgggtcgag gaagcacggt tcctgatgcc
     1501 gatgccacgc ctcttcgacg gcttcgtcga atacaccaag cgggtctctc cgacctgcct
     1561 ggtccatctg gagcgcaacc gctacagcgt gccggcgtcc ttcgccaacc ggccggtcag
     1621 cctcagggtc tatccggaac ggattgtggt tgccgcggaa gggcagatcg tatgcgagca
     1681 ctgccgcatc atcgagcggt cgcatgacgg ccccggtcgc accgtctacg actggcgtca
     1741 ctatctggcg gtcatccagc gcaagcccgg tgctctgcgc aacggcgctc ccttcaccga
     1801 actccccgaa gccttcaaac ggctgcagca gcatctgctc aggaagcccg gcggcgaccg
     1861 cgagatggtc gagatcctgt cgctcgtcct gcagcatgac gaacagatcg tgctgacggc
     1921 cgtggagatg gcattgcagg ccggggtgcc gaccaaggtc cacatcctca atctcctgca
     1981 ccggctggtt gacggcaagc cgatcgagac gccgatcgtg gatgcgccgc aggctctgag
     2041 cctggccaag gaaccgcaag ccaatgtcga gcgctacgat gcgttgcgcc agacccggga
     2101 ggtgcgccat gcgtcatgat cccgccagcg gcgctatcgt catcatgctc cggagcctca
     2161 aaatgcacgg catggcacag gccgtcgccg agtttaccga acaggcttct cccgccttcg
     2221 aggccgccat ccccatcctg tcgcaactcc tgaaggccga gatggccgag cgggaggtca
     2281 ggtccgtagc ctatcagctc aaggcagccc gcttcccggt ctatcgcgac ctggcaggct
     2341 tcgacttcgc cagcagcgag atcaacgagg ccctcgtgcg acagctccac cgatgcaagt
     2401 tcatggacga tgccaacaat gtcgtgcttg tcggcggccc gggcaccggc aagacccata
     2461 tcgctaccgc ccttggcgtg caggcggtcg agcatcatcg caagcgtgtc cgcttcttct
     2521 ccaccgtcga
//
LOCUS       DASZYN010000471         5268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1240996, whole genome shotgun sequence.
ACCESSION   DASZYN010000471 DASZYN010000000
VERSION     DASZYN010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240996"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VHE33_12175"
     CDS             <1..308
                     /locus_tag="VHE33_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98255.1"
                     /translation="ATPSPEHNALSFPTPEQTRAESWPELCRRFLVGAEKSAAIANCT
                     DTEIKVLCPSPENPARSMTVREQLESFAAHNAYHLGRVVLLRQILGSWPPPSGGLTW"
     gene            360..2033
                     /locus_tag="VHE33_12180"
     CDS             360..2033
                     /locus_tag="VHE33_12180"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HVT98256.1"
                     /translation="MRRSLLLGLAFAVLMAGRWVGAANGQAGAVDDEAQGKAWWAHIQ
                     VLASDSMRGRQTGSQDYMRAAAYVVKQFKADGLAPAGERGSYFQPVHFVEQWVNAGKS
                     SLSLVVDGHAQPLALGQDAVLGAHGPQPKTVDAGLVFIGYGLHLPESGYDDFNSAEVS
                     AASLRGKIVVVINGGPADLPTALKSYARTTPMKKAMRDAGVVGIVSIPTPKSMDFGWE
                     RIASSASQPGMWLAPAAGDAEVARRHPALADDHDPMFSAAFNPAQAEKLFAGTGHTFA
                     EMLALADAQKPLPRFDLKKRMTATVMTKKATVVSPNLVAKLEGSDPSAGYVAVSAHLD
                     HLGVGEPIKGKTIYNGAMDDASGVATVLEAAQALSRDAAKGIRPRRSILFVIFTAEEK
                     GLLGSRYFAGHPTVPEKEIRADLNLDMFMPLFAFRKLHVQGLEQSTLQDDARKVGEEH
                     HVIIAGDPEPDRNSFIRTDQYSFVQAGVPALAMKFGWEPGSPEYHMWRDWLKERYHST
                     QDDLNQPVDLVAAAQFNSFFADLVRAVANDPAMPHYLEGSFFHRFENGQ"
     gene            2183..2473
                     /locus_tag="VHE33_12185"
     CDS             2183..2473
                     /locus_tag="VHE33_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98257.1"
                     /translation="MVTAWRSVAFGAGVAAGVVCLIAVMACLLVRFPMIPAPQGHYLV
                     NPRRDLVMTIALFTGVLTVVLAMFGTGLRRMFLAGGGLVLLGLWYSAWMLRF"
     gene            2499..3308
                     /locus_tag="VHE33_12190"
     CDS             2499..3308
                     /locus_tag="VHE33_12190"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HVT98258.1"
                     /translation="MNRREVIRTGLAASAAMMAESAARSQANMPNAAGKGRQYYELRK
                     YLLRSGGQTQLTADYLSKALLPALNRMGIGPVGAFTVEIGPATPTVYLLLPSANLETL
                     VGAGLHLREDQTFLSAAEPFWSAPDSRPAFERVESTLLIAFEGWPQLVVPPPTAQNGS
                     RKFQLRSYESAGDKDHVRKVQMFHSGEFEIFEKAGFWRVFFGDALIGERLPQLTYMLS
                     FPDLEHMNAMWDKFRNDPDWKKLQADPRFSYGAIVSNIVNLILSPTTYSQV"
     gene            3311..3781
                     /locus_tag="VHE33_12195"
     CDS             3311..3781
                     /locus_tag="VHE33_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98259.1"
                     /translation="MAAARKSARTASGAVKKAAPPSAGYSGTPLWKKLGVKDGQLTWR
                     LQMPASVAEEIAAGGVAPKLVKAPKAGLEMAHLFVTGKRELAEHLDWLRGVLAQDGTV
                     WVSWPKKASGVATDITEDAVREVGLPIGFVDIKVCAVNAVWSGLKLVIRKTERK"
     gene            complement(3800..4345)
                     /locus_tag="VHE33_12200"
     CDS             complement(3800..4345)
                     /locus_tag="VHE33_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98260.1"
                     /translation="MPPRKAASAKRKAAPHPAKKSPAKKPASGASPAAQLDSFLDKFT
                     PEIAAEARVALRRMRARLPGAQELVYDNYNALAIGFSPTERTSEALFSIALFPRWVSL
                     FFLQNGTRLRDPDCLLEGSGNQARHIKLDKGAMIDDPGVQDLIAQALELAEKQIDPGQ
                     PRRLIIKSVSAKQRPRRPAGR"
     gene            complement(4430..4987)
                     /locus_tag="VHE33_12205"
     CDS             complement(4430..4987)
                     /locus_tag="VHE33_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98261.1"
                     /translation="MSIKVDDVEGSRQIRIYQRRTSIPLVLLAIMVSVYAGALLRFFP
                     AYREHPPDAAVLVGGIPMILLLAFSTVMANKKWFGGYQRVTVTPSTMEVESRLLGFTT
                     SRHQYMNQAVRKLRYEEWYPQTGRALARARTHGVRFEYDGVTQAIAVDAESADCMDLI
                     DRMIEVFSFSTAPSSEPEMATSLFP"
BASE COUNT          991 a         1473 c         1803 g         1001 t
ORIGIN      
        1 gggcgacacc ttctccggag cataatgccc tgagctttcc gactcccgag cagacgcgcg
       61 ccgagtcgtg gccggagctg tgccggaggt ttctggtggg cgcggaaaag tccgccgcca
      121 ttgcaaactg cacggacacg gagataaagg tgctttgccc gtcgccggaa aatccggcgc
      181 gcagcatgac ggtgcgtgaa caactggaga gctttgccgc gcacaacgcg tatcacctgg
      241 ggcgcgtcgt gctgctcaga cagatattgg gaagctggcc tccgccttca ggcggattga
      301 cctggtgaca actcgccatc gaggcgggaa cgggccactt tgaccgaggg ggtaagagga
      361 tgcgtcgttc gctgctgttg ggattggcat tcgcggtgct gatggccggc agatgggttg
      421 gagcggccaa cggtcaggca ggtgcggtcg acgatgaggc gcaggggaag gcgtggtggg
      481 cgcatattca ggtcctcgcg tccgactcga tgcgcggccg acagaccgga tcacaggatt
      541 acatgcgcgc agcagcgtat gtggtcaagc agttcaaggc cgatgggctg gccccggcgg
      601 gcgagcgtgg cagctatttt caaccggtgc atttcgtcga gcagtgggtg aatgcgggca
      661 agtcgtccct gagtttggtg gtcgatgggc acgcgcagcc tctggcactg gggcaggatg
      721 cggtactcgg tgcgcatggg ccacagccga agacggtgga cgcaggattg gtattcattg
      781 ggtacgggct gcatctgccg gaatcgggct acgacgactt caactcggcg gaggtatcgg
      841 ccgcctcact ccgcggcaag atcgtcgtag tgatcaacgg cgggccggca gatctgccca
      901 ccgcgctgaa atcgtatgcg cgcacgacgc cgatgaaaaa agccatgcgc gatgctggtg
      961 tcgtcggcat cgtctcgatc cccacgccga agtcgatgga ttttggctgg gagcggatcg
     1021 cctcgagcgc atcgcagccg ggcatgtggc tggcccccgc cgcgggcgat gcggaggtgg
     1081 cccgccggca tcccgcgctg gccgacgatc acgatcccat gttctcggcg gcgttcaatc
     1141 cggcgcaggc ggaaaagctg tttgcgggaa cagggcacac cttcgcggag atgctggcgc
     1201 tggccgatgc gcagaaaccg ctgccgcgct tcgacctgaa gaagcgaatg acggcgacgg
     1261 tgatgacgaa aaaagctacc gtggtctcgc cgaatctggt ggcgaaactg gaggggtcgg
     1321 atccgtccgc ggggtatgta gcggtgtcgg cgcacctcga tcacctcggc gtcggcgagc
     1381 ccatcaaggg caagacaatc tacaacggcg cgatggatga cgcctcgggc gtggcgacgg
     1441 ttctcgaggc agcgcaggcc ctgagcaggg atgcagccaa gggcattcgc ccgcggcggt
     1501 ccatcttgtt tgtcattttt accgcggagg agaagggcct gctggggtcc agatatttcg
     1561 cagggcatcc cacagtgccg gagaaggaga tccgggcgga tctgaatctt gacatgttta
     1621 tgcccttgtt tgcgttcagg aaattgcatg tgcaggggct ggagcagtcg acgttgcagg
     1681 acgatgcgcg gaaggtgggc gaggagcatc atgtgataat cgcgggcgac ccggaaccag
     1741 accgcaattc ctttatccgg accgatcagt acagctttgt gcaagccggc gtgccggcac
     1801 tggcgatgaa gttcgggtgg gagccgggtt cgccggagta tcacatgtgg cgtgactggc
     1861 tgaaggagcg ctaccactcg acgcaggacg atctgaatca acccgtggac ctggtggcgg
     1921 cggctcagtt caattcattt tttgccgatt tggtgcgagc ggtggcaaac gaccctgcga
     1981 tgccgcatta tctggaggga agtttctttc accggtttga gaacgggcag tagtcgcagg
     2041 ggaggaatgc gagtgaatcg aagaaaacga atcacggcgg cttcggcggc ggcaatgccg
     2101 aagcggggga cggtttcgca ggcggctgcg ggctgttgcg ccggctgatg ggcagctggc
     2161 cgccgccctc gggtgggctc acatggtgac ggcatggaga tcggtcgcgt tcggagccgg
     2221 cgtcgccgcg ggagtcgtgt gcctgatcgc tgtgatggcc tgcctcctgg tgcggtttcc
     2281 gatgatccct gcaccccagg ggcactatct ggtgaatccg cgcagggacc tggtaatgac
     2341 catcgcattg ttcaccggcg tgttgacagt ggtgttggcc atgttcggga ccgggttgcg
     2401 gaggatgttt ctggccggag gcgggctggt gttgctgggg ctctggtact cggcatggat
     2461 gttaaggttt tgaggaacac acacacgttg ggggacagat gaatcgtaga gaagtcattc
     2521 ggacagggct ggcggcttcg gcagccatga tggcggaaag cgctgcgcgc tcgcaggcga
     2581 acatgcccaa cgctgccggc aagggccgcc aatattacga gctgcgcaaa tatctgctgc
     2641 gttccggcgg acaaacacaa ctgacggcgg attatctgtc gaaggcgctc cttccggcgc
     2701 tgaatcgcat gggaattggc ccggtgggcg cgttcaccgt cgagattggc cccgcgacgc
     2761 cgacggtgta tctgctgctg ccatcggcga acctcgagac gctggtcggg gccggactgc
     2821 atctccgcga ggatcagacg ttcttatcgg cggccgagcc gttctggagc gcgccggatt
     2881 cgaggcccgc ctttgagcgg gtcgagagca ctttgctcat tgcttttgaa ggatggccac
     2941 agctggtcgt gcctccgccg accgcgcaga acgggtcgcg gaaattccag ctccgtagct
     3001 acgagagcgc cggcgacaag gaccacgtgc gcaaggtgca gatgttccac tccggagagt
     3061 tcgagatttt tgagaaggcc ggcttctggc gggtgttttt cggcgatgcg ctgatcgggg
     3121 agcggctgcc ccagctcacc tatatgctca gctttcccga tctcgagcat atgaacgcca
     3181 tgtgggacaa gtttcgcaac gatccggact ggaagaagct gcaggcggat ccccggttca
     3241 gctacggagc catcgtgtcg aacattgtga acctgatcct gagcccgacg acgtactcac
     3301 aggtgtgacg atggccgccg cgcgcaagtc agctcgaacg gcttctggcg cagtgaagaa
     3361 ggctgcgccg ccttcggctg gttattcggg caccccgctc tggaagaagc tcggggtgaa
     3421 agacggccag ctcacgtggc ggctgcagat gccggccagc gtagccgaag agatcgccgc
     3481 aggcggcgtt gcaccgaagt tggtgaaggc gccgaaggcc gggctcgaga tggcgcattt
     3541 gtttgtgacc gggaagaggg aactggcgga acatctggac tggctccgcg gcgtgctcgc
     3601 acaggatggg acggtctggg tgtcgtggcc gaagaaggct tcaggcgtgg caacggacat
     3661 caccgaagac gcggtgcgcg aggtaggtct gccgatcggc ttcgtggata ttaaggtgtg
     3721 cgcggtcaac gcggtctggt cggggctgaa gctggtgatc cgcaagacgg agcggaagta
     3781 gtccggcttt tgctcgttgc tatctgcccg ccggcctgcg cggccgctgc ttcgccgaga
     3841 cggatttgat aatcagccgc cgaggctgtc ccggatcaat ctgcttctcg gccagctcca
     3901 gcgcctgcgc aatcaggtcc tgcaccccgg gatcgtcgat catggctcct ttgtcgagct
     3961 tgatgtgacg cgcctggttg ccggaaccct ccagcaagca gtccgggtcc cgcagccggg
     4021 tgccgttctg caggaagaac aggctgaccc agcgcggaaa gagtgcgatc gaaaacagcg
     4081 cctccgaggt gcgctcggtg ggcgagaagc cgatcgccag cgcgttgtag ttgtcataga
     4141 cgagttcctg ggcgccgggc aggcgcgcgc gcatgcggcg cagcgcaaca cgcgcctcgg
     4201 cggcgatctc gggagtgaat ttgtcgagaa agctgtcgag ctgggccgcc ggggaagcac
     4261 ccgaggccgg cttcttggcc gggctcttct tcgccgggtg tggtgcggcc ttcctcttcg
     4321 cggacgcggc cttgcgcgga ggcatggtgc gaggaatatt agcagggaag gctgggaagg
     4381 tacatgggct taccacatct gccaaaatcg ggcacccagg cccgccctac tatggaaaca
     4441 ggctcgtagc catttctggc tccgaggacg gcgcggtcga aaagctgaag acctcgatca
     4501 tgcgatcaat cagatccatg caatcggccg actcagcatc cacggcaata gcttgggtca
     4561 ccccgtcata ttcgaagcgc acaccatgtg tacgggctct cgccaaagcc ctgcccgtct
     4621 gcggatacca ctcttcgtac cttagtttcc ggaccgcctg gttcatgtat tgatgacgcg
     4681 atgttgtgaa ccccaggagc cgtgattcga cctccatcgt cgaaggcgtc acagtgactc
     4741 gctgatagcc cccgaaccat ttcttgttgg ccatgacggt gctgaaggcg aggaggagaa
     4801 tcattgggat ccctccgacc aggaccgcag catcaggcgg atgctcgcga taggccggga
     4861 agaacctcaa aagtgcccct gcatacacag aaaccataat cgccagcagc actaacggaa
     4921 tagatgttct gcgctgatat atccggatct gccggctacc ctctacatca tccactttga
     4981 tcgacatttg tgatccaggc cgtcaacacg atttatagcg ttgagggcta ccgggggcac
     5041 atacaccttt cgtaggaaag gctcgagtca agaccttggc gtccgtggga cacctgccaa
     5101 acggatgggg gaaggtcggg agaaattccc cgcaaaaggg gaaagcccgc tccggggtgg
     5161 gtggagcggg ctttcggggg tgcggcaagg ggtagttgcc gctttgcatg tttatgccgg
     5221 cgatcaccta atctcccaca cactttcgcg tgcagtacca tcggccca
//
LOCUS       DASZYN010000472         6951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_472485, whole genome shotgun sequence.
ACCESSION   DASZYN010000472 DASZYN010000000
VERSION     DASZYN010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472485"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..754
                     /locus_tag="VHE33_12210"
     CDS             113..754
                     /locus_tag="VHE33_12210"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVT98262.1"
                     /translation="MILETFPVGPLQCNCTILGDESTGEAMVVDPGDNIPQILAFLAQ
                     HKLTLKQIVITHGHIDHVGGAARLKQATGAPVLMNQHDLEQLKMMDVQAAWIGVQPPE
                     VTPPDYSAEEGMTVGIAGHSAQVLYTPGHTQGSICLHFVPDNLLLAGDTLFAGSIGRT
                     DLPGGDSKQILRSLHDRLLTLPDETHVIPGHGPGTTIGEERQSNPFLQPGVRF"
     gene            complement(829..1536)
                     /locus_tag="VHE33_12215"
     CDS             complement(829..1536)
                     /locus_tag="VHE33_12215"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HVT98263.1"
                     /translation="MIYVRPRLQTFAAAALLCLLTPSFARASQVSDTARITASGDVLK
                     ELLDSPSGISHPLLNKAYCVIVLPSVKKGGFILAAQYGRGIMTCRSGNNFDGPWSAPI
                     MMQSSGGSIGFQIGGQETDFVILVMNSKGARAVMNGKAKLGSDASVAAGPVGRTAEAS
                     TNASMGAEMLSYSRSQGVFAGVSLSGASLGPDDDSNQQIYGQPISGRAIFAGGVDAPE
                     AASMLLSTLKAVSPTRN"
     gene            complement(1607..2308)
                     /gene="pyrF"
                     /locus_tag="VHE33_12220"
     CDS             complement(1607..2308)
                     /gene="pyrF"
                     /locus_tag="VHE33_12220"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HVT98264.1"
                     /translation="MDSRLIVALDFPGAAPALALADRLNGLCRWFKVGLELYLAEGNA
                     IVHELRRREFSVFLDLKFHDIPNTVAGAVRSAAAAGAQMLTVHSAGGPAMLEAAAEAA
                     ACVPDPPQLLAVTVLTSMDQLQLSAIGIDHSPETQVLRLADAAAAAGINGFVASPEEV
                     SALRNRFSGATLVIPGIRPAGAAVGDQKRVATPATALAAGASFLVIGRPITQAADPES
                     AARAILDEMAQVRID"
     gene            2545..4107
                     /locus_tag="VHE33_12225"
     CDS             2545..4107
                     /locus_tag="VHE33_12225"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HVT98265.1"
                     /translation="MRWTLAIMVFVIVCIVCGCKVGSSAGGAVASQTDNGAIPDNTIS
                     GTVTLNGSPLAGATVTLFNTNYNVVAGTATTDASGNYRFTALPATGNVPTEYQLWAQK
                     TGYGFYPSAGSGAKVMRWDYTGQFQGNGLTDAGIYFTVIDYFSLPHAPLTGADFIASD
                     GSTPRVHLAATGQTASFASGDDGDLHQGTAWGASRFTDNGDGSVTDTLTGLVWLKDAG
                     CLPAANWATAITEATALKSGACGLTDGSKAGDWRLPNLNELESLVDVSAAHPAVTVGH
                     PFLHVSNGIYWSSTSYFGGVGGSPLAWAIRMDDGRYINDVDGKLNNKSTASNAVWAVR
                     GSGTGGAVKLAATGYYDEPGQVVQGDDGNLQTGVGLSYARFIDNGDGTLTDTMTGLVW
                     MKKADCINGTWEDAVAAVRALGNGACSLTDHSQPGDWRVPNRNEMQSLQDRMVNNVAD
                     FMNATYVWKSDAAFYRGPLFTSFHGYEYYWTSTTDAADTTAAWTVFSCDFGVYDIPKS
                     SAGFALAVRDKR"
     gene            4296..5747
                     /locus_tag="VHE33_12230"
     CDS             4296..5747
                     /locus_tag="VHE33_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98266.1"
                     /translation="MKPMETSIESRLNFIRSGPRTDGVVPPTVTGAMPSIQRAPAKAP
                     EISAREIGADRALDTAGVSMPAPELLSPDQRPRCAAELGIRPSIVEDLALKSLYVGGA
                     MSTREMARQMRLSVNVADELVNKMRTSQLCQVTGMTANLATVALTDQGRKRAQELMAL
                     SQYVGAAPVSLESYVSQVRKQSVRNLVVRKADVERAFGDLVIDPKVLGQIGTGLNSGA
                     SIFVHGPAGVGKTAIAETMSRVLAEDDVWIPYSVEVDGQMIMVYDPMIHKRVDSPQFD
                     SCDERWVRCQRPSVLVGGELTIDMLDLQFNATTRFYSGPVQMKANNGVLIMDDFGRQR
                     VRPEDLLNRWVVPLDRGIDFLTMAGGRKIEIPFEMLVVFSTNLDPADLVDAAFLRRMQ
                     TKIRVDTATDDQFRQIFRRVAARRGLSVDEGALTDLIRAIREGLHQELRACQPRDLVN
                     QVCWAAQYEDREPFLDRASLFCAMEAYFLTPKA"
     gene            complement(5960..6460)
                     /locus_tag="VHE33_12235"
     CDS             complement(5960..6460)
                     /locus_tag="VHE33_12235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HVT98267.1"
                     /translation="MNAIERRDNGTHPHRLWILAGTILATILGVAGARAASPATHLNP
                     QIIVSRHHWYQLGVASWYGKFFAGRPTASGEIFDPHQLTCAHRTLPLGSVLRVTNLRN
                     HKSVIVTVNDRGPVPEDRVIDLSKAAANALDFTDQGLAPVKVELIGPQDELAQLLMPA
                     GLEPAR"
     gene            6736..>6951
                     /locus_tag="VHE33_12240"
     CDS             6736..>6951
                     /locus_tag="VHE33_12240"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HVT98268.1"
                     /translation="MPDSALTPAVLTIAGYDPSSGAGITADLEVFHDHGVPGVSAVTA
                     LTVQSRSGVRRVEPVNPALLLETLELLS"
BASE COUNT         1391 a         2206 c         2157 g         1197 t
ORIGIN      
        1 caagtagccc tccgctcgga agacggaagc gcaggcaaaa tcaaaagggc aggcctttgg
       61 cctgtccttt ttctgcgccc tgcgacctgt gccctgtgat ctgatgagaa gcatgatcct
      121 cgagaccttt cccgtcggcc cactgcagtg caactgcacc atcctcggcg acgaatccac
      181 gggtgaggct atggtcgtcg atccgggcga caacatcccg cagatcctcg cctttctcgc
      241 gcagcacaag ctcacgctga aacagatcgt catcacccac ggccacatcg atcacgtcgg
      301 gggcgccgcc cgcctgaagc aggcgaccgg ggcgcctgtc ctgatgaacc agcacgacct
      361 cgaacagctg aagatgatgg atgtgcaggc cgcctggatc ggagtgcagc cgcctgaagt
      421 caccccaccc gactacagtg cggaagaggg catgaccgtc ggcatcgccg gccactccgc
      481 gcaggtgctt tatacgccgg gccacacgca gggcagcatc tgccttcact ttgtgcccga
      541 caatctactc ctcgctggag acaccttatt tgccggttcc attggaagaa ccgacctgcc
      601 cggcggcgac tcgaagcaga tcctgcgttc gctccacgat cgtcttctca ccctgccgga
      661 tgagacgcac gtcatccccg gccacggccc cggaacgacc atcggcgaag agcggcagtc
      721 gaatccgttc ctccagcccg gcgtgcggtt ttgatctctg cgcgaagctg cgcaaatgcc
      781 aggcacaccg ttccctgagc tgcggctgat tcgaaaattc gacgcttatt aattgcgcgt
      841 aggcgaaacg gctttcaggg tcgagagcag catactcgcc gcctccggag catccactcc
      901 accggcgaag attgcgcgcc cgctgatcgg ctggccgtag atctgctggt tggaatcgtc
      961 atcgggtccg agcgaggctc ccgacaacga gacgccggcg aaaacgccct gcgagcgcga
     1021 gtaagagagc atctcggcgc ccatcgacgc attcgtcgaa gcctcggcgg tgcgccccac
     1081 cgggccggcg gccacgctgg catcggatcc gagtttcgcc ttgccgttca tcaccgcgcg
     1141 cgcgcccttg ctgttcatca ccaggatgac gaaatcggtc tcctgcccgc cgatctgaaa
     1201 accaatgctg ccgccgctgg actgcatcat gatgggcgcg gaccacggtc cgtcgaagtt
     1261 gttgccgctg cggcacgtca tgatgccgcg gccgtattga gccgcgagga tgaagccgcc
     1321 cttcttgacc gagggcagaa cgatgacgca gtacgccttg ttcagcagcg ggtgcgaaat
     1381 tccgctcggg ctgtcgagca gctctttcag cacgtcgccg gatgcggtga ttctggccgt
     1441 gtcggaaacc tggctggcgc gggcgaacga cggcgtgagg agacaaagaa gagcagcagc
     1501 ggcgaaggtc tgcaggcgag gacgaacgta gatcacgcga ctttcctttc gtgtggccgg
     1561 acggatccgc aaagcgggcc tgccaggaat ccacagcggt ttcatcctaa tcgatcctga
     1621 cctgggccat ctcgtcgaga atcgcgcgcg ccgcagactc aggatccgcc gcctgcgtaa
     1681 tggggcgtcc aatcacgagg aagctcgcgc ccgccgccag cgcagtcgcg ggcgtagcca
     1741 cgcgtttctg atcgcctacc gccgcaccgg ccggccgaat gccgggaatg acgagggtcg
     1801 cgccggaaaa gcgattccgc agcgcagaga cttcttccgg actcgccacg aagccgttga
     1861 tcccggctgc cgcggcggca tccgcaaggc ggagcacctg cgtctccggt gagtgatcga
     1921 tgccgatggc ggaaagctgg agctgatcca tgctcgtgag gacggtgacg gcgaggagtt
     1981 gcggaggatc gggcacacag gcagcggcct cggcggccgc ttccagcatg gccggcccgc
     2041 cggccgagtg aaccgtcagc atctgcgcgc ccgccgcagc cgcggagcgc accgcgccgg
     2101 ccaccgtgtt cggaatgtcg tgaaacttca ggtcgaggaa gaccgagaac tcgcgacggc
     2161 gcagttcgtg cacgatcgcg ttgccttcag ccaggtagag ttccagcccg accttgaacc
     2221 agcggcagag gccgttgagc cggtcggcca gagccagagc aggcgctgcg cctggaaaat
     2281 cgagagcgac gatgagacgg gaatccatta ggaaccagcg tacagggagt ggcgaaccca
     2341 tgcaggtgaa ggggggcggg ttcgggaacg ggacgaaggt cggaagggat cccaaattga
     2401 gacaatgcac cgcgcgccct gggtgaattc gcatcaagtc ccgcgaaatt cctccgatga
     2461 tcctttcggc ggttctcccc actcctgctt ccggagtgca gagagcgcca tcggttctga
     2521 aagagggcct tcatgcggag cgggatgcgt tggactctgg cgatcatggt gttcgtgatt
     2581 gtttgcattg tctgcgggtg caaagtggga tcgagcgcgg gcggcgccgt ggccagccag
     2641 acggacaacg gagcgatccc ggacaacacc atctccggaa cggtaacgct caacggaagt
     2701 ccgctggccg gcgcaacggt gacgctgttc aacacgaact acaacgtcgt cgccggaacg
     2761 gcgacgaccg acgccagcgg caactaccgc ttcacggcgc tccccgcaac cggcaatgtg
     2821 cccaccgaat atcaactgtg ggctcagaag accggatacg gcttctatcc gagtgcggga
     2881 tcgggcgcaa aggtgatgcg ctgggattac acgggccagt tccagggaaa tggcctcacc
     2941 gacgccggca tttacttcac ggtgatcgac tacttttcgc tgccccacgc gcctctcact
     3001 ggcgcggatt ttatcgcctc ggacggcagc acgccgcgcg tgcatctggc agcgaccggg
     3061 cagactgcca gcttcgcgtc cggcgatgac ggcgacctgc accagggaac cgcgtggggc
     3121 gcctcgcgct tcaccgataa cggcgacgga tcggtcaccg acacgctgac cggcctcgtg
     3181 tggctcaaag acgcgggctg cctgcccgcc gccaactggg ccacggcaat caccgaagcg
     3241 acggccctga agagcggcgc gtgcggcctg accgacggat cgaaggcggg cgactggcgt
     3301 ctgccgaacc tgaatgagct ggagagcctc gtcgacgtct ccgcggccca tcccgcggtc
     3361 acggtcggcc acccgttcct gcatgtttca aacggaatct actggtcctc caccagctac
     3421 ttcggtggcg tgggcggatc gccgctcgcg tgggcgattc gcatggacga tggccgctac
     3481 atcaacgacg ttgacggcaa gctgaacaac aaatccaccg ccagcaatgc cgtgtgggcc
     3541 gtccgtggct ccggtacggg cggagccgtg aaattagccg caaccggcta ctacgatgag
     3601 cccggccagg tcgttcaggg cgacgacggc aacctccaga ccggcgtcgg cctgagctac
     3661 gcgcgcttca tcgacaacgg cgatggcacg ctcaccgaca ccatgaccgg actggtgtgg
     3721 atgaaaaagg cggactgcat caacggaacc tgggaagatg ccgtcgccgc ggtccgggca
     3781 ctcggcaacg gagcctgcag cctgaccgac cattcccaac cgggcgactg gagagtcccg
     3841 aaccgcaacg agatgcagag cctgcaggac cgcatggtga acaacgtcgc cgatttcatg
     3901 aatgcgacgt acgtgtggaa gagcgatgcc gccttctacc gcggccccct cttcaccagc
     3961 ttccatggat atgagtacta ctggacgtcg accaccgacg ccgccgacac caccgcagcg
     4021 tggacggtgt tcagctgcga cttcggcgtc tacgacattc cgaaaagcag cgccggcttc
     4081 gcgcttgcgg tccgggacaa gcgataggaa gaaggtcatg aagcactgca gtcattcgcg
     4141 tgcgcccttt ggtggagcgc gtttccaaat gggccagccg agggacacct tatcaaaaag
     4201 gtaattaccc gaaagggcaa tccagtttcc gtaacaaacg gattctaatg gccaatgaca
     4261 cacgggccga cggagacaat aaggggaaga attctgtgaa gcctatggaa acatcgatcg
     4321 aatcgcgact caatttcatt cgatcgggac cgcgcaccga cggcgttgtg ccgccgacag
     4381 tcactggcgc gatgcccagc attcagcggg caccggcaaa ggctccggaa atatccgcac
     4441 gcgaaatcgg cgccgatcgt gcgctcgaca cggccggcgt ttccatgccc gctcccgagc
     4501 tgctgtcgcc cgatcagcgt ccgcgttgcg cggctgaact ggggatccgt ccctcgatcg
     4561 tcgaagacct cgcgctcaag agcctctatg tgggcggagc catgtccacg cgggagatgg
     4621 cgcggcagat gcgcctgagc gtgaacgtcg ccgatgagct cgtgaacaag atgcgcacca
     4681 gccagttgtg ccaggtcacc gggatgaccg ccaacctggc gacggtggcc ctgaccgacc
     4741 agggcaggaa gcgcgcccag gaactcatgg cgctgagcca gtacgtgggc gcggcgccgg
     4801 tttcgctcga gagctacgtt tctcaggtcc gcaaacagag tgtgcgcaat ctcgtcgtgc
     4861 gcaaggccga tgtggagcgc gccttcggcg atctggtcat cgaccccaaa gtgctgggac
     4921 agatcggcac cgggctcaac tccggagcct caatcttcgt ccatggaccg gctggcgtgg
     4981 gcaaaaccgc catcgccgaa accatgtcgc gggtgctggc cgaagacgac gtctggatcc
     5041 cttactcggt cgaagtcgac ggccagatga tcatggtcta cgacccgatg atccacaagc
     5101 gggtcgacag ccctcagttc gacagttgcg atgagcgctg ggtccgctgc cagcggcctt
     5161 cggtgctcgt cggcggcgag ctcaccatcg acatgctcga tctgcagttc aacgcaacga
     5221 cccggttcta cagcgggccg gtgcagatga aggccaacaa cggcgtcctg atcatggacg
     5281 acttcggccg ccagcgggtc cgccctgaag atctactcaa ccgctgggtg gtgcctctgg
     5341 atcgcggcat cgacttcctc accatggccg gcggccggaa aatcgaaatt cccttcgaga
     5401 tgctggtcgt cttctcgacc aatctcgatc ccgccgacct ggtcgacgcg gcgtttctcc
     5461 gccgcatgca gaccaagatt cgcgtggata ccgccaccga cgatcagttc cgccagatct
     5521 tccgccgcgt cgcggcccgc cgcggcctca gcgtggacga aggcgcactg acggacctga
     5581 tccgcgccat ccgcgaaggt ctccatcagg aactccgcgc gtgccagccc cgcgacctgg
     5641 tgaatcaggt ctgctgggca gcgcaatatg aggaccgcga gcccttcctc gatcgcgcct
     5701 cgctgttctg cgccatggaa gcgtacttcc tcacgcctaa agcgtaaagg gcagtccaac
     5761 tcagcaaacc gcttcagacg cttgacccgc gggcagacct tctgacgccg aacatgggtt
     5821 tcccagcctg catgggttca ggaccgtggg caggcagccg cccgcacgtc tccggacccc
     5881 aggcactcac aaatctgcac gcacacccac tgtggagggc gtgaggctgg ggtcggaaac
     5941 aaatcgaaac gagggtcggc tagcgcgccg gctcgaggcc ggccggcatc agcaactggg
     6001 ccagttcgtc ctgcggccca atcagctcga ccttcactgg cgccaggccc tgatcggtaa
     6061 agtcgagggc attcgcggca gctttggata aatcgatgac ccggtcttcg gggacgggtc
     6121 cgcggtcatt caccgtgaca atgaccgact tgtggttgcg caggttcgtg acgcgcagca
     6181 ccgatccgag gggcagcgta cgatgagcgc aggtcagctg atgcggatcg aagatctcgc
     6241 cgctggcggt cggccggcct gcaaaaaact tgccgtacca ggaagcgaca cccagctgat
     6301 accagtgatg gcggctgacg atgatctgag ggttgagatg tgtagccggg gaagcggccc
     6361 gcgcaccggc gacgcccaaa atcgtggcga gaattgtgcc ggcgagaatc cataatcgat
     6421 gggggtgagt gccgttatct cttcgttcga tagcgttcat actcctcctc ctgaaattgt
     6481 aggaatcatg tgcttgtaag tcaaacaaag tagaactatt taacccacaa gaacacttgc
     6541 gtactgcctg catttgtggt agtcaggaat gcaggattga ctgccaaaaa agcagcggcc
     6601 ttttcttccg gatgtccctg ccaaaacggg ccgaaatgaa aagacctgaa agtacgatgg
     6661 cggaatacgt cagcgtgctg ccacgaatga atatggtccc cgattttgcg cctcaaacct
     6721 aatggtgtca agcacatgcc agactcggca ttaacgccgg cagtacttac gatcgcgggt
     6781 tacgatcctt cctccggtgc cggaatcacg gccgacctcg aagtctttca cgatcatggt
     6841 gttccggggg tttcggctgt tacagcgctg accgtccagt cccgcagcgg cgttcgacgc
     6901 gtcgagccgg tcaatccggc tttgttattg gaaacactgg agttgctctc a
//
LOCUS       DASZYN010000473         4400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_985952, whole genome shotgun sequence.
ACCESSION   DASZYN010000473 DASZYN010000000
VERSION     DASZYN010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985952"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..519)
                     /locus_tag="VHE33_12245"
     CDS             complement(139..519)
                     /locus_tag="VHE33_12245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT98269.1"
                     /translation="MHRRVLIIDDEEDIREVAALSLETVAGWDVLVAGSGAAGIAKAR
                     ENRPDAILLDVMMPGMDGPTTFRELRSLPETANIPVILLTAKVQAADQKRFSDLGVVK
                     VMFKPFDPLVLAQEIAATLGWQEI"
     gene            complement(697..3378)
                     /locus_tag="VHE33_12250"
     CDS             complement(697..3378)
                     /locus_tag="VHE33_12250"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVT98270.1"
                     /translation="MTRFSHSRTVTSAFMIALAFSLIGAIILARYGSSPGQSHPWIFA
                     FACFWTLALALLVTISLSTFRATLRERQGERQLQRVARLHRSILDSAGPMMIACDLTG
                     RINLFNPAAERMLGYRANEAVDKLSASDLFPESEMARVGEQLAASMPTFRAHPEIPPH
                     TAPALLSYVRYIATFPASRVRGFEMRYRRRDGSTFPAMVYLSAVRSSAGTVEGLVTIA
                     IDLSATRRAEHALRESEERYRDLFENSPEMIATLSPRGRYLYVNPSWQALFGMDSSQF
                     ESLIGFESPFPPEVQAEAAALFHKALHGVRVEQHPLRLQNSSGENVEVEASLSCRREE
                     TGPVSVRCIFRDVTQQNRRERRLRIQLVISQIIAETTSIDEGQTRVLEALCQTFACEF
                     AALWTVDESQHSIRSCATWFAKGRPSDDFRHETETLTLSRGQDLPGTVWSLGKPCWIS
                     DLRQHPAFLRRTTARLNGYVSGWAVPVRVGNQVIAVVECFSRQFMREDPDLLATVETV
                     CGSVGQFMARAGQEIRVRQLDRQKAVILNSVADGIIGVDSTNRIAFANPAAARMLECN
                     ESELIGLRVHDLLHPPIAGCDSNCLTRRAFQTAEGASGQDVYFRGKGKSFPVEFSLMP
                     MVEEGASVGSVLSFRDISQRYALDHMKDEFISTVSHELRTPLTSIRGALGLLSAGLLG
                     SVSEKATSLLRIAVANSDRLVRLINDILDLERMTSGRAPLSFRRLSLDDLVRQAIETM
                     QPVADAEQVYLTSDCNSVFIEADSDRLTQVLTNLLSNAIKFSPPRSTVRVAATRASDG
                     VTISIIDEGRGIPTDKLESIFDRFQQVDASDSRQKGGTGLGLAICRTIVQQHGGRIWA
                     EQNNSRGAIFRFFLPERASISPLAEEATQNQFEQRLG"
     gene            complement(3564..>4400)
                     /locus_tag="VHE33_12255"
     CDS             complement(3564..>4400)
                     /locus_tag="VHE33_12255"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF002699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HVT98271.1"
                     /translation="DLVRNNMVDAIVSTGANIVDQDFFEALGFQHYIADDLYKSGTQD
                     GELRDLMIDRIYDTLIDEEELRACDETTEKVADSLPPRPHSSREFIRAMGAWLEANGK
                     TPEKGGADSIVFSAYQKDVPIFCPAFSDCSAGFGLVAHQHKRQDKPKVSIDSAKDFYE
                     LTQLKIANPTTGLLMIGGGVPKNFAQDIVVAADILGNEAPMHKYAIQITVADVRDGAL
                     SSSTLKEASSWGKVDTTFEQMVYSEATLALPLITGYAWHKKAYAERRERRWSRILEPV
                     TV"
BASE COUNT          830 a         1132 c         1633 g          805 t
ORIGIN      
        1 ggattgcggg tgaggctgtc gcaggcagag tggagaatcg ccatgcgttc catgatcgtc
       61 ggacggctgg tgcgccacag ctcggtcagg cgtgcggcga ttttggtggc agcatcctcg
      121 ttcatcgctg ctgccagttt agatctcctg ccagccgaga gtcgcggcaa tctcctgggc
      181 gagcacgagc ggatcgaagg gcttgaacat caccttcacc acgccgagat cggagaagcg
      241 cttctggtcg gcggcctgca ctttggcggt gagcaggatg acggggatgt tggcggtttc
      301 cggaagggag cgcagctcac ggaaggtggt agggccgtcc atgcccggca tcatgacatc
      361 cagaaggatg gcgtcgggcc ggttttcgcg ggccttggcg attccggcag cgccggagcc
      421 ggccaccagc acatcccagc cggcaacggt ttccaggctg agcgctgcga cctcgcgaat
      481 gtcttcttcg tcgtcaatga tcaaaacacg tcgatgcatt tcttctttca attcccgatt
      541 caactattaa atcaatttgt ggattcccac catcccccgc tcgttctacg accaggggta
      601 ccagcgggag gcgagcggaa ggcgagaaga agacgagcga acaacgagcg gaaggcgaga
      661 agaaggcgag aaggaaaggg cgaagaggat agctgtttat ccgagccgtt gctcgaactg
      721 gttctgcgtg gcttcttcgg cgagcggaga gatggaggcg cgctcgggaa ggaagaagcg
      781 gaagatggcg ccgcgcgagt tgttttgctc ggcccagatg cggccgccgt gctgctgaac
      841 gatggtgcgg cagatggcga gtccgagacc ggtgccgccc ttctggcgcg agtccgaagc
      901 gtcgacctgc tggaatcggt cgaagatgga ttcgagcttg tcggtgggaa tgccgcggcc
      961 ttcgtcgatg atgctgatgg tgacgccgtc ggaggcgcgg gtggcagcga cgcgcacggt
     1021 ggagcggggc ggagagaact tgatggcatt gctgagcagg ttggtgagca cctgggtaag
     1081 gcggtcggag tcggcttcga tgaaaacgga attgcagtcg ctggtgagat agacctgttc
     1141 ggcgtcggcg accggctgca tggtttcaat cgcctggcgt acgaggtcat cgagggaaag
     1201 gcgacggaag ctgagcgggg cacggccgga ggtcatgcgc tcgaggtcca gaatgtcgtt
     1261 gatgaggcgg acgaggcggt cggagttggc gacggcgatg cgcagaaggc tggtggcttt
     1321 ttcgctgacg ctgccgagca ggcccgcgga cagaagaccg agcgcgccgc gaatcgacgt
     1381 gagaggcgtg cgcagctcat ggctgaccgt ggagatgaac tcatccttca tgtggtcgag
     1441 ggcatagcgc tggctgatgt cgcggaagct gagcacgctg ccgaccgagg cgccctcttc
     1501 gaccatgggc ataagggaga attcgacggg aaagcttttg cctttgccgc ggaaatagac
     1561 gtcctggccg gaggcgcctt cggcggtttg gaaggcacgg cgggttagac agttggagtc
     1621 gcagccggcg atgggcggat ggagcaggtc atggacgcgc aggccgatca gctcggactc
     1681 attgcattcg agcatgcgcg cggcggcggg attggcgaag gcgatgcggt tggttgagtc
     1741 gacgccgatg atgccgtcgg cgaccgagtt caggataacg gccttctgcc ggtcgagctg
     1801 gcgaacgcgg atttcctggc cggcgcgcgc catgaactgg cccaccgagc cacaaaccgt
     1861 ttcgacggtg gccagcaggt cgggatcttc gcgcatgaac tggcgggaga agcactcgac
     1921 aacggcgatg acctggttgc ccacacggac gggcacagcc catccggaaa catatccgtt
     1981 gagccgggcc gtggtgcggc gaagaaaggc gggatgttgg cggaggtcgg agatccagca
     2041 gggcttgccc agcgaccaga cggttccggg gagatcctgg ccgcgggaga gggtgagcgt
     2101 ctcggtttcg tggcggaaat cgtcgctggg acggcctttg gcaaaccacg ttgcgcagga
     2161 acgaatcgag tgctgcgatt catcgacggt ccagagtgcc gcgaactcgc aggcgaaggt
     2221 ctggcagagg gcttcgagaa cgcgggtctg cccttcgtcg atggaggtgg tttcggcaat
     2281 gatctggctg atgacgagct gaatgcggag gcggcgctcg cggcggttct gctgggtgac
     2341 gtcgcggaag atgcagcgga ccgagaccgg gccggtttct tcgcggcgac agctcaggct
     2401 ggcttcgact tcgacgtttt cgccggagga gttctgcagg cggagagggt gctgttcgac
     2461 gcggacgcca tgcagggctt tgtgaaagag ggctgcggct tcggcctgca cttcgggcgg
     2521 gaacggggac tcgaatccga tgagggactc gaactgggag gaatccatgc cgaacagggc
     2581 ctgccaggag gggttgacgt agaggtagcg gccgcggggg ctgagggtgg cgatcatctc
     2641 cggcgagttt tcgaagaggt cgcgatagcg ctcttcactt tcgcgcaggg cgtgctcggc
     2701 gcggcgcgtg gcgctcagat cgatggcgat tgtgaccagg ccttccacgg ttccggcgga
     2761 ggagcgcacg gcggacaaat agaccatggc gggaaaagtg ctgccgtcgc ggcggcgata
     2821 gcgcatctcg aatccgcgaa cccggctggc ggggaaggtg gcgatatagc ggacataaga
     2881 aagaagcgca ggcgcggtat ggggcgggat ttcaggatgc gctcgaaagg tgggcatgga
     2941 ggcggcgagt tgctcgccga cgcgggccat ttcgctctcg gggaagaggt cggatgcgga
     3001 aagcttgtcg acggcttcat tggcgcggta gccgagcatc cgttcagcgg ccggattaaa
     3061 gaggttgatg cggccggtga gatcgcaggc aatcatcatg ggaccggcgg agtcgaggat
     3121 gctgcgatgg agacgggcca cgcgctggag ctgccgctcg ccctggcgtt cgcgcaaagt
     3181 cgcccggaag gtggagaggg aaatggtgac cagcagggcg agggcgaggg tccagaagca
     3241 ggcaaaagca aagatccagg gatggctctg gccgggggag ctgccgtacc gcgcgaggat
     3301 gatggcaccg atgagggaaa acgccagcgc gatcatgaaa gccgacgtga cggtgcggga
     3361 gtggctgaat cgggtcacga ctcgattccg tggaggtccg gaatgtggat ttccctctaa
     3421 tttcggcagg gatgcgaggc ttcagtatag gggaagccct ggagttccga caggaaaatc
     3481 cacagcgaac ccgctcagca atcccacttt ggtgttacct gtggcagagc cgagcggtta
     3541 ataaatttgt aactgtcacc gggctaaacc gttactggtt cgagaatccg gctccagcgg
     3601 cgctcgcggc gttcggcgta agccttctta tgccaggcat agccggtgat gagaggcagg
     3661 gccagagtgg cttcgctgta aaccatctgc tcgaaagtgg tgtcgacctt gccccaggag
     3721 ctggcttcct tgagcgtgct cgaggagagg gcgccgtcgc ggacgtcggc cacggtgatc
     3781 tggatggcgt atttgtgcat gggcgcttcg ttgccgagga tgtcggccgc gaccacgatg
     3841 tcctgcgcga aattcttggg cacaccgccg ccgatcatga gcaggccggt ggtgggattg
     3901 gcgatcttga gctgcgtcag ctcgtagaag tccttcgccg aatcgatgga gaccttcggt
     3961 ttgtcctgcc gcttgtgctg gtgggcaacg aggccgaagc cggccgaaca gtcgctgaag
     4021 gcggggcaaa agatggggac gtctttctga tatgcagaga agacgatgga atcggcgccg
     4081 cccttttccg gggtcttgcc gttggcttcg agccaggcgc ccatggcgcg gatgaattcg
     4141 cgcgagctgt gggggcgggg cggaagcgag tcggcgacct tctcggtggt ctcgtcgcag
     4201 gcacggagct cttcctcgtc gatgagggtg tcgtagatgc ggtcgatcat cagatcgcgg
     4261 agttcgccat cctgggtgcc ggacttatag aggtcgtcgg cgatgtagtg ctgaaagccg
     4321 agagcttcga agaagtcctg atcgacgatg ttggcgccgg tggagacgat ggcgtccacc
     4381 atattgttgc ggacgaggtc
//
LOCUS       DASZYN010000474         7983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_472492, whole genome shotgun sequence.
ACCESSION   DASZYN010000474 DASZYN010000000
VERSION     DASZYN010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7983
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472492"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VHE33_12260"
     CDS             <1..315
                     /locus_tag="VHE33_12260"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVT98272.1"
                     /translation="GDTGFCDVFAALRSRFERVRLALLPIGAYEPEWFMGPVHMTPEQ
                     AVEAHEILGAATSMGIHFGTFALADDGETAPVERLQRALASRKDQTRFWTLSEGEGRL
                     VP"
     gene            complement(507..2036)
                     /locus_tag="VHE33_12265"
     CDS             complement(507..2036)
                     /locus_tag="VHE33_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HVT98273.1"
                     /translation="MQTAVATAKVLIGGEWRESDAEGVFEPENPATGEKLGREFPVSR
                     WSDCDKALSAAVEAARALETTPAEKIAAFLEAYAKELELDADAIVQAAHEETALALSP
                     RLKDVELPRTTDQLRQAAAAARQGTWRRPIIDQAKNLRSCYAPIGPVVVFGPNNFPFA
                     FNGVSGGDFAAAIAAGNPVIAKAHPLHAYTSQLMAEAATRALKSTGLHPATVQMIYKL
                     NHENGARLVSDPRVGAAGFTGSKGAGLKLKHAADSAGKPIYLEMSSLNPVLFLPEGLK
                     ENAEKWARELADSCTMGSGQFCTRPNLALVIASDAAEAFVKELTVAIASREPHALLSS
                     TSRDSLHESVEALQHAGAKLLTGGKNVPGKGYRYMSTLLRVSGKEFLAAAEELQREAF
                     GNEVMAIVAADEAELLQVLEHLEGQLTGTVYSAASGADDALYARVAPVLRRKVGRLLN
                     DKMPTGVAVSPAMNHGGPYPATGHPGFTAVGIPTSIVRFTALQSYDNVRTERLPEFLR
                     G"
     gene            complement(2196..3020)
                     /locus_tag="VHE33_12270"
     CDS             complement(2196..3020)
                     /locus_tag="VHE33_12270"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HVT98274.1"
                     /translation="MILYRTAEGLVLQEGDQRFAIEETLDGLLMQADVHEYLAQKTQG
                     AGTARAMDTTKLLAPIGSQEVWAAGVTYLRSRAARMAESKDAGGGDFYDRVYSAERPE
                     LFFKSLPHKVVGPGGTVGLRSDATWSVPEPELALLIDPRGRIVGYTIGNDMSSRDIEG
                     QNPLYLPQAKVYNRSCALGPGILMTKEPLSRETRISIEIERGGSVVFSGETSVAQIKR
                     PFEELAMYLYRDQTFAFGTFLLTGTGIVPPDSFTLEKGDTVRIRIDGIGELMNPVG"
     gene            complement(3017..5023)
                     /locus_tag="VHE33_12275"
     CDS             complement(3017..5023)
                     /locus_tag="VHE33_12275"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: protein motif:HMM:TIGR03432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhG/YagF family D-xylonate dehydratase"
                     /protein_id="HVT98275.1"
                     /translation="MTSIPVGEVLETQELDLRAVATHAPGPVGALPLTPEMLLTQPSG
                     NLFGLTMNAGMGWDPARMGGPEVLILSTHGGLRAEDGTPIALGFHTGHWEVGLLVAEA
                     ARALKGLHAVPFAGACTDPCDGRTQGTTGMLDSLPYRNDAAIVLRRLMRSLPTRAGVL
                     GIATCDKGLPAMMMALASSRELPAVLIPGGVTLLPESGEDAGKIQTIGARFAQGQISL
                     EYAAEMGCRACASPGGGCQFLGTAATSQVVGEALGLALPHTALAPSGQAIWLDAAKRS
                     AAALLRLREIGVGTQDILTQAAFENAMVVYAAFGGSTNLLLHVPAIAHAAGRKRPEVA
                     DWMRVNREVPRLVDALPNGPRGFATVQVFLAGGVPEVMLHLRAAGLLHTKALTAAGVT
                     LGACLDWWEQSERRRALQQNLRERDGIDADEVICSPDRARSRGLTSTVCFPKGNLAPE
                     GSVVKSTAIDPSLIREDGVFFHRGPARVFMLESDAIEAIRKGQIVAGDVMVLLCCGPL
                     GAGMQEIYQITAALKALPHCRHVSLITDGRFSGVSTGPCIGHISPEALAGGPVGKLRE
                     GDTVEIRIDRQKLSATVDLVGENGEVFGAEEGRRRLTARESRPDLRPHPALPDDTRLW
                     AAMVEASGGIWGGCVYDVDAITARLRGTAVSERTREQEEARETT"
     gene            complement(5083..6357)
                     /locus_tag="VHE33_12280"
     CDS             complement(5083..6357)
                     /locus_tag="VHE33_12280"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT98276.1"
                     /translation="MVTPSETDLRKRWSRILPAVFITYSLAYLDRANYGFGAAAGLAQ
                     TLHISGYQSSLLGALFFLGYCLPQIPGVILVQRYSARLLIFVALIGWGAFAALTGVIR
                     SFWMLVADRVLLGMAESFVFPALLIVLTRWFTRAERSRANALMILANPVTVLWMSALT
                     GFLIHAWGWQVAFILEGMAAVGWGVFWLVLMRDHPAQVSWLSVEARGDLEEALEHEQR
                     QLPRFENFWPAFREPNVIRLCINYFFWSVGIYGFVLWLPVVIRNATKAGMGKTGLISA
                     VPYLVSVGVMMTVSHFSDRRGQRQKYVWPLLMLGGVALAGSFFTVDRSFVVAYACLIV
                     SACCMYAPYGPFWAMVPELLPRNVAGETMALINCVGALGGFFGTYFVGVLQTYTGSSA
                     AGFLLMSLSLILSGVMLLGVKLPERQPVASSQ"
     gene            complement(6429..>7983)
                     /locus_tag="VHE33_12285"
     CDS             complement(6429..>7983)
                     /locus_tag="VHE33_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98277.1"
                     /translation="PASLPELPADQQNQRRGVIYSVAASYKNTQTIWAGTDDGLLWIT
                     RDGGANWSQITPPGLTEWSRIAQIDASRFDDDTAYVAVNRMRIDDMKPYAFRTHDGGK
                     SWQSISAGLPEDGPVDAIRADPVQPGLLYAATEKAVLVSFDDGGHWQSIEYNLPHTSM
                     RDVLVKDDDLIVATHGRSFWILDDVAPLREIAAQAGEKMPLLFKPADAWRVRSAEYTD
                     TPMPLDEPRGENPPDGALIDYDLQSPAQDVTLEVLSSDGAVLRKYSSHDPVTPTPEEM
                     KKDLIPPYWPMVHGPLATTAGMHRWVWDLRSTTPTATRYSYPISAVPHRTPLNPQGPL
                     VLPGQYTVRITVDGHSETQTITVKMDPRVHTSPADLEALHTAQMTMAQTLDAVAKADL
                     AAHAVAEQMNAPQNASLGTQLQTYKDALKLVLEGDHAAHRPGIDEVNGEAGQLYGQLE
                     QSDNPPTEALTQAAAHVQEEGKKAVPAWEEFREKQLPELNKVLAAAHHARINLTKPPS
                     DMPEEGDEE"
BASE COUNT         1516 a         3004 c         2016 g         1447 t
ORIGIN      
        1 ggcgacaccg gcttctgcga tgtgtttgct gctctgcgca gtcgcttcga aagagtccga
       61 ctggccctgc tccccatcgg cgcctacgag ccggagtggt tcatgggccc ggtgcacatg
      121 acgcccgagc aggccgtcga ggcgcacgaa atcctcggcg cagctacttc gatgggcatt
      181 cacttcggta cttttgctct ggcagacgat ggagaaacag cgccggttga acgcctccag
      241 cgtgcgcttg caagccgcaa agaccaaacc cgattctgga cgctcagcga aggcgaaggc
      301 cgcctcgttc catagagtcg tcctggtcga gtcgtcctgg tcatttcacg gcttgtctag
      361 caatgaatgg gatcggttca gcccggaatg gtggcgtctt ctcgcgaggg aggagacgcc
      421 agggcgcgag ggcgggcacc cagggccccc gcgaaacctc gtttcgccgg ggtggcgatc
      481 caggatgcca ggctgcgaac cggaaactat ccccgcaaaa actccggcag ccgctccgtc
      541 cgaacattgt cgtagctctg caaagccgtg aaccgcacaa tcgacgtcgg aattcccacc
      601 gccgtgaatc ccggatggcc cgtcgccgga tacgggccgc cgtgattcat cgccggactc
      661 accgccacgc ccgtcggcat cttgtcgttc agcagccggc ccaccttgcg ccgcagcacc
      721 ggcgctacac gcgcgtacaa cgcatcatcg gcgcccgacg cagccgaata caccgtcccc
      781 gtcaactgcc cttcgaggtg ctccagcacc tgcagcagtt ccgcctcatc ggcagccaca
      841 atcgccatca cctcattgcc gaacgcctcc cgttgcaact cctccgccgc cgccaaaaac
      901 tccttgccgg aaacccgcag cagcgtgctc atgtaccgat agcctttgcc cggcacgttt
      961 ttcccgccgg tcagtagctt ggctcccgca tgctgcagcg cttcaaccga ctcatgcagt
     1021 gaatcccgcg acgttgacga cagcagtgca tgcggttcgc ggcttgcgat ggccactgtc
     1081 agctccttca cgaacgcctc ggccgcgtcg ctcgctatca caagcgcgag attgggacgc
     1141 gtgcagaact gccccgaccc catcgtgcag ctgtccgcca gctcgcgcgc ccacttctcg
     1201 gcgttctcct tcagaccttc cggcaaaaac agcaccgggt tcaggctcga catttcgaga
     1261 tagatcggct tcccggcgct gtccgcagca tgcttcagct tcagccccgc gcccttgctc
     1321 cccgtgaacc ccgccgcgcc gacccgcgga tcgctcacca accgcgcccc gttttcatgg
     1381 ttcagcttgt agatcatctg caccgtcgcc gggtgcagtc ctgtgctctt caacgcacgc
     1441 gtcgcagcct ccgccatcag ttgcgaggtg tacgcgtgca gcgggtgcgc cttcgcgatc
     1501 acaggattcc ccgccgcaat cgccgcggca aaatcgcccc cgctgactcc gttaaaggca
     1561 aatggaaagt tgttcggtcc aaaaaccacc accggcccaa tcggcgcgta acacgatcgc
     1621 aaattcttcg cctgatcgat aatcggccgc cgccacgtcc cctgccgtgc ggccgccgct
     1681 gcctgccgca gttgatcggt cgtgcgcggc agttccacat ccttcagcct cggcgagagc
     1741 gccagcgctg tctcctcatg agccgcctgc acgatcgcat cggcatccaa ctccagttcc
     1801 ttcgcatatg cctcaaggaa cgcagcaatc ttctccgccg gagttgtctc cagcgcacgc
     1861 gcggcctcca cagccgccga cagcgccttg tcgcaatcgc tccaccggct caccgggaac
     1921 tcccgtccca gcttctcccc cgttgctgga ttctccggct cgaacacgcc ctccgcgtcc
     1981 gactcccgcc actctccgcc gatcaaaacc ttcgccgtcg ccactgccgt ttgcatgccg
     2041 tctcctcgct attagagcgc tccacaagcc tttaggattc acagcgctga cccgcttctg
     2101 cccttgctcg ctgttctctc gctctttgct cgtcttttac tcgctgtccg ctcgcctctt
     2161 gctcgccgtt cgctcgcttc ccgcttctag cttctctatc ccacgggatt catcagttcg
     2221 ccaatgccat cgatcctgat ccgcaccgtg tcgcctttct ccagcgtaaa ggaatcgggc
     2281 ggcacaatgc ccgtcccggt cagcagaaac gtgccgaacg caaacgtctg atcccgatac
     2341 aggtacatcg ccaattcctc aaacggccgt ttgatctgcg caacagacgt ttctccggaa
     2401 aatacgacgc tgccgccccg ctcgatctca atcgagatgc gcgtctcccg cgacaaaggt
     2461 tccttcgtca tcagaatccc cggccccagc gcgcagctcc ggttgtagac cttggcttgc
     2521 ggcaaataaa gcggattctg cccctcaata tcgcgcgagc tcatgtcatt cccgatcgtg
     2581 tagcccacaa tccgcccgcg aggatcgatc agcagcgcca gctccggctc cggcaccgac
     2641 cacgtcgcat cgctgcgcaa tcccaccgtg ccgccgggtc ccacaacttt gtgcggcagc
     2701 gatttgaaaa atagttccgg ccgctcggcc gaatacaccc gatcgtagaa atccccaccg
     2761 cccgcgtcct tcgactccgc cattcgagcc gcccggctcc gcaggtacgt cacccccgcc
     2821 gcccaaacct cctggctccc aatcggcgcc agcagctttg tcgtatccat cgctcgtgcc
     2881 gtcccggcgc cctgggtctt ctgcgccaga tactcgtgca catcggcctg catcaacaaa
     2941 ccatccagtg tctcttcaat cgcaaacctc tggtcgcctt cctgcaggac gagcccctcc
     3001 gccgttcgat acagaatcac gttgtctccc tcgcttcttc ctgctcgcgc gttctttcac
     3061 tcactgccgt tcctcgcaga cgcgcggtga tcgcatccac atcgtaaaca cacccgcccc
     3121 agatcccgcc gctcgcttcc accatcgccg cccacaaccg cgtatcgtcc ggcagagcgg
     3181 gatgcggccg cagatccggg cgcgactccc gcgctgtcag ccgccgccgt ccttcttccg
     3241 caccaaaaac ctcgccgttc tctcccacca gatcaaccgt cgcgcttagc ttctggcgat
     3301 cgatcctgat ctcgaccgtg tcgccttccc gaagctttcc caccggccct cccgccagtg
     3361 cctcgggcga aatatgcccg atgcacggac ccgtcgacac cccactgaag cgcccgtcag
     3421 tgatgagaga aacatggcgg caatgcggca gtgccttcag cgccgcggtg atctgataga
     3481 tctcctgcat tcccgcgccc agcggaccac agcagagcaa aaccatcacg tcgcccgcca
     3541 cgatctgccc tttgcgaatc gcctcaatcg cgtcgctctc cagcatgaac acgcgcgctg
     3601 gcccgcggtg aaaaaacacg ccgtcttcgc gaatcagact cggatcgatc gccgtgctct
     3661 tcaccaccga tccctccggc gccaggttcc ccttcggaaa acacaccgtc gacgtcagcc
     3721 cccgcgatcg cgcccgatcc ggcgagcaga tcacctcgtc agcatcgatc ccgtcccgct
     3781 cgcgcagatt ctgctgcaga gcgcgccgcc gctcgctttg ctcccaccag tcaaggcacg
     3841 cacccagcgt taccccagcc gccgtcagcg ccttagtgtg cagcagcccc gcggctcgca
     3901 aatgcagcat cacctccggc acgccgcctg caagaaacac ctgcaccgtc gcaaatccac
     3961 gtggtccatt cggcagagca tccaccaacc gcggcacttc acggttcacg cgcatccaat
     4021 ccgccacctc aggccgcttg cgtcccgcgg catgcgcgat cgctggaaca tgcagcagca
     4081 gattcgtcga accaccgaat gccgcataga cgaccatcgc attctcaaag gctgcctgcg
     4141 tcaggatgtc ctgtgttccc accccaattt cccgcagacg cagcaatgcc gccgccgagc
     4201 gcttcgctgc gtcgagccag atcgcctgtc cagacggagc cagcgccgtg tgcggcaatg
     4261 cgagtcccag cgcctcaccc acaacctgcg aagttgccgc cgttcccaga aactggcatc
     4321 caccgcccgg cgatgcgcac gcacggcagc ccatctccgc tgcgtactcc agtgagattt
     4381 gcccctgtgc aaagcgcgcg ccgatcgtct gaatcttccc cgcatcctcg ccactctcgg
     4441 gcagcagcgt cacgccgccc ggaatcagca ccgccggcaa ctcgcgtgac gacgccagcg
     4501 ccatcatcat tgccggcaat cccttgtcgc acgttgcgat gcccagcacg cccgctcggg
     4561 tcggcagcga tcgcatcagc cgccgcagca cgatcgccgc gtcattgcga tacggcagcg
     4621 aatccagcat ccccgtcgtg ccctgtgtgc gcccgtcaca cggatcggtg caggctcccg
     4681 caaacggcac cgcatgcaga cccttcagcg cccgcgctgc ttctgctacc agcaatccca
     4741 cttcccagtg cccggtgtgg aaaccgagtg ctatcggcgt cccgtcttcg gcgcgcagtc
     4801 ccccatgcgt gctcaggatc aggacctccg gcccgcccat ccgtgccgga tcccaaccca
     4861 tccccgcatt catggtgagc ccaaacaaat ttcccgaagg ctgcgtcaac aacatctcgg
     4921 gcgtgagcgg aagcgcaccc accggtcccg gcgcatgggt cgccactgcg cgcagatcca
     4981 gctcctgcgt ctccagcacc tcacccacag gaatcgacgt catcgcaact cactcacctg
     5041 accacccacg ggcaacctgt ggctacattc aaatccacaa ggctactggc tactggctac
     5101 tggctgccgc tcaggcaact tcaccccgag caacatcact cccgacagaa taagcgagag
     5161 cgacatcagc agaaaccccg ccgccgagct gcccgtataa gtctgcagta ccccgacaaa
     5221 atacgttccg aagaatccac ccagcgcgcc gacgcagttg atgagcgcca tcgtttcccc
     5281 ggccacattg cgcggcaaca actctggcac catcgcccag aacggcccgt acggcgcata
     5341 catacagcac gccgacacaa tcaggcatgc gtacgccacc acaaaagacc ggtccaccgt
     5401 gaagaacgat cccgccaggg ccacgccgcc cagcatcagc agcggccata cgtatttctg
     5461 ccgctgtccc cgccggtcgg aaaagtgcga gaccgtcatc atgaccccga cagatacaag
     5521 gtacggaact gccgaaatca aaccagtctt ccccatcccc gccttggtcg cattccgtat
     5581 caccaccggc agccacaaca caaatccgta aatccccacg ctccagaaga aataattgat
     5641 gcacaggcga atcacgttcg gctcacgaaa cgccggccag aaattttcaa agcgcggcaa
     5701 ctgcctctgc tcgtgttcga gcgcctcctc cagatcgccc ctggcttcca cgctcagcca
     5761 tgacacctgc gcgggatggt cgcgcatcag cacgagccaa aacacgcccc atcccactgc
     5821 agccatgcct tcaaggatga acgccacctg ccatccccag gcgtgaatca gaaatcccgt
     5881 cagtgccgac atccataaca ccgtcaccgg attcgccagg atcatcagcg cattcgctcg
     5941 cgaccgttcc gcacgcgtaa accagcgggt cagtacgatc agcagcgccg gaaacacgaa
     6001 gctctccgcc atgcccagca gcacgcgatc cgccaccagc atccagaagc ttcgaataac
     6061 gcccgtcagc gcagcaaacg cgccccaccc gatcagcgcc acaaagatca gcagccgcgc
     6121 gctgtatcgc tgcaccagaa tgacgccggg aatctgcggc agacagtatc caagaaagaa
     6181 aagcgcgccc agcagcgacg attgataccc gctgatgtgc agcgtctgcg ccagccccgc
     6241 cgccgccccg aagccgtagt tggcacgatc caggtacgcc aggctgtacg tgatgaatac
     6301 cgcaggaaga atgcgactcc atcgcttccg cagatccgtc tcgctgggag tgaccatcag
     6361 ttccttgtgt ccaggcaccc cagtagatca catctgtttc gtgaacgctg aacgccgagc
     6421 actgaacgct actcctcgtc tccctcttca ggcatatccg acggcggctt cgtcagattg
     6481 atccgcgcat gatgcgcagc cgccagcacc ttgttcagct ccggcaactg cttctcgcgg
     6541 aattcctccc acgccggcac agctttcttc ccttcctcct gcacatgagc cgcagcctgc
     6601 gttagcgctt ccgtcggcgg attgtcggac tgctccagct gcccatacag ctgtcccgcc
     6661 tcaccgttca cttcatcgat gcccggacga tgcgccgcat gatccccttc aagcaccagc
     6721 ttcagcgcat ccttatatgt ctggagttgt gtaccaagcg acgcgttctg cggcgcgttc
     6781 atctgctccg ccacggcatg cgcggcgaga tcggccttcg ccactgcatc gagtgtctgc
     6841 gccatcgtca tctgcgccgt atgcagcgct tccagatcgg ccggcgaagt gtggacccgc
     6901 ggatccatct tcaccgtgat ggtttgcgtt tcgctgtgac cgtccaccgt gatccgcacc
     6961 gtgtattggc caggcaaaac cagcgggccc tgcggattca gcggcgtccg gtgcggcacc
     7021 gccgaaatcg gatacgaata ccgcgtcgct gtcggcgtgg tcgaccgcag atcccacacc
     7081 caacggtgca tccccgccgt cgtcgccagc ggtccatgca ccatcggcca atacggcgga
     7141 atcaggtcct tcttcatctc ctccggcgtc ggcgtcaccg gatcgtgact cgaatacttc
     7201 cgcagcaccg caccatccga actcagcact tccagcgtca cgtcctgcgc gggcgactgc
     7261 aggtcgtagt cgatcaaagc tccatccgga ggattctccc cgcgcggctc atccagcggc
     7321 attggggtgt ccgtgtactc cgcactccgc acccgccagg catcggccgg cttgaacagc
     7381 aacggcatct tctcgcccgc ctgcgcagcg atctcgcgca gaggagccac gtcgtcgaga
     7441 atccagaacg accgcccgtg cgtcgccaca atcaaatcat catccttcac caggacatcg
     7501 cgcatcgagg tgtgcggcag gttgtactca atcgactgcc agtggccacc gtcatcgaac
     7561 gaaaccagta cggctttttc cgttgctgca tacaacaacc ccggttgcac cggatccgca
     7621 cggatcgcat ccaccggacc atcctccggc aacccggcac tgatcgactg ccacgacttg
     7681 cccccatcgt gcgtgcggaa tgcatacggc ttcatgtcat cgatgcgcat gcgattcaca
     7741 gccacgtacg ccgtgtcgtc gtcgaaccgt gaagcatcaa tctgcgcaat cctgctccat
     7801 tccgtcagtc ccggcggggt gatctgcgac cagttcgccc caccgtcccg cgtgatccac
     7861 agcaacccat cgtcggttcc cgcccagatc gtctgcgtgt tcttgtacga agccgcgacc
     7921 gaatagatca caccccgccg ctgattctgc tgatccgccg gcaactcagg cagcgacgcc
     7981 ggc
//
LOCUS       DASZYN010000475         4997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1179200, whole genome shotgun sequence.
ACCESSION   DASZYN010000475 DASZYN010000000
VERSION     DASZYN010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179200"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VHE33_12290"
     CDS             complement(<1..960)
                     /locus_tag="VHE33_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98278.1"
                     /translation="MNLRHLGLAAAACATTLLWTSFAPGQEQDGDKVDLAALTQIKNE
                     AFQHSQVMENLFYISEVYGPRVNNSRNHRAAAEWAMKQMQSWGLQNVHLEKWGPFGDG
                     WQIKKYYGALETPAYASLIGFPLAWTPGTNGPVTADAVLAPLHSADDFAKYKGKLRGK
                     IVLIFDPRELTLHTEPEARRLTDEEIEARTKTPDPAHMGFAFLRPGGAAPRPGEYTPA
                     TLSTAAPTGRVLRNQVNAFLKEEGVAVALTPGYNGDGGTIFASYGGSQDPKDPVGPPM
                     AAITPEQYNRICRLIQHGITPKLTFDIDVEYQKDDPMGFNVIGE"
     gene            1335..2039
                     /locus_tag="VHE33_12295"
     CDS             1335..2039
                     /locus_tag="VHE33_12295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HVT98279.1"
                     /translation="MSTAIAGPREQTREPRAPSFWQSTNGKKAVMAVTGAILFGFVVG
                     HLAGNLQIFDGPARLNAYGKFLKSLGELLWIARGILIVSVVLHIWATVQLALRNMKAR
                     PVGYKVKKSVASNYADRTMYWSGPIILAFVIFHLLQFTAGTLHPGAATFSDTDIYYNV
                     VAGFSVWWVSAWYILSMILLGFHLRHGIWSAFQTLGANHPRHTPVLKQAALWVAILIT
                     VGYISIPVAVLAGWVR"
     gene            2042..3961
                     /locus_tag="VHE33_12300"
     CDS             2042..3961
                     /locus_tag="VHE33_12300"
                     /inference="COORDINATES: protein motif:HMM:NF005749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HVT98280.1"
                     /translation="MAIKLDARIPSGPIEQAWDNARFEMKLVNPANKRRHHLIVVGSG
                     LAGGAAAATLGELGYNVKCFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSIYRLFYDT
                     VKGGDFRAREANVYRLAQNSVHIIDQCVAQGVPFAREYGGTLDNRSFGGAQVSRTFYA
                     RGQTGQQLLLGAYQALERQVDAGQVTMIPRTEMLDLIVIDGQARGIVTRNLITGKLAV
                     HLADAVILATGGYGNVYYLSTNAKGSNTTAIWRAHKHGALFANPCFTQIHPTCIPVTG
                     DYQSKLTLMSESLRNDGRIWVPKQKGDKRKPDQIPEAERDYYLERKYPAFGNLVPRDV
                     ASRNAKAVCDEGRGVGETGLGVYLDFAEAIGRLGVNTIRERYGNLFDMYQRITDEDPY
                     KVPMRIYPAIHYTMGGLWVDYQLQSTIPGLFVLGEANFSDHGANRLGASALMQGLSDG
                     YFIIPYTVGNYLATAKPKPVKPDHPAAQAALAATEQGIAKLLAIKGKRTVDSFHRELG
                     KIMWDDCGMSRNAKGLEEAIGKIRALREEYWQNVNVPGSGDDLNQSLEKAGRVADFLE
                     LGELMCIDALDRDESCGGHFREEYQTPEGEAQRDDEHFSYVAAWGWTGPGNPPELNKE
                     PLEFAYVHPSQRSYK"
     gene            3961..4758
                     /locus_tag="VHE33_12305"
     CDS             3961..4758
                     /locus_tag="VHE33_12305"
                     /inference="COORDINATES: protein motif:HMM:NF005746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HVT98281.1"
                     /translation="MKVTLTIWRQKNAAAQGKFVSYSVNVENGEMSVLELLDVLNESL
                     IEKGEEPVAFDHDCREGICGMCGFMINGVAHGPLPATTICQLTMRHFKDGEELILEPW
                     RSAAFPFVKDLVVDRRSFDRIIQAGGYVSISTGSAPDGNAIPVGKEDADRAMDAAACI
                     GCGACVAACPNGSAALFTGAKIAHLGILPQGKPEEDHRAVRMVAQTNIEGFGSCTNIG
                     ECTGVCPKEIPLEVIAKMNRDYLAGAWKERKEVIHAIAPVRSWEMEK"
BASE COUNT          983 a         1653 c         1446 g          915 t
ORIGIN      
        1 ctctccgatc acgttgaagc ccatcggatc gtccttctga tattcgacgt cgatatcgaa
       61 cgtcagcttc ggcgtgatgc cgtgctggat caggcggcag atgcggttgt actgttccgg
      121 cgtgatcgcc gccatgggcg gtccgaccgg atccttcgga tcctgcgatc ctccatagga
      181 cgcaaagatc gtaccgccgt cgccgttgta gccaggggtc agcgccacgg ccacaccctc
      241 ttccttcaag aaagcgttca cctggttgcg cagaacgcgc cccgtcggcg ctgcggtcga
      301 aagcgtcgcc ggtgtgtatt cgccgggccg aggggccgcc cctcccggcc gcagaaacgc
      361 aaagcccatg tgtgccggat ccggcgtctt cgtgcgcgcc tcaatctcct cgtcggtcag
      421 gcggcgcgct tctggttccg tatgcagcgt gagctcgcgc ggatcgaaga tcagcacgat
      481 tttgccgcgc aatttgcctt tgtacttggc aaaatcgtcc gccgaatgca acggcgccag
      541 caccgcgtcc gcggttaccg gtccgtttgt gcccggagtc cacgccaagg gaaacccgat
      601 gagcgaggca taggccggcg tctccagcgc cccgtaatat tttttgatct gccacccatc
      661 gccgaacggc ccccacttct ccaggtgcac attttgcagt ccccacgact gcatctgctt
      721 catcgcccat tcggccgccg cacgatgatt gcggctgttg ttgacgcgcg gtccatacac
      781 ttcggagatg tagaacaggt tctccatcac ctgcgaatgc tggaaagcct cattcttgat
      841 ctgcgtcaga gcggccaggt ccaccttatc gccatcctgt tcctgtccag gcgcgaagga
      901 agtccacaac agggtcgtgg cgcaggcggc agcggccaac ccgagatgac gcaggttcac
      961 gatgatttcc ctttcgaaga tgaagttgcg ttggactgcc cgtgaaaacg gggaagctcg
     1021 gcgctgtaca tccgaaccgc gctttcacaa cttgctgctg gaaattcggg gacggtgacc
     1081 agggtattga atttagtccc atgatgcaaa tggaaagtcc gcgggcaggg tcccgcaccg
     1141 cgatctcagt gtctctgcac cggggacgcg tttgcccaga cggcggtcct ccttgacgct
     1201 ggttattccg gacaccaata ataaagggtt gcactcttta tcagttttcc ccacacacgc
     1261 ggctgccacc ggccgccccc aactacgcaa agaggaatcc ccgacgtgat gaaatccagg
     1321 gctggacaag cgcgatgagc acagcaatcg ctggaccgcg cgaacagact cgtgaaccca
     1381 gagcgccgag tttctggcaa tccaccaacg gcaagaaagc agtcatggcg gtgacggggg
     1441 cgatcctgtt cggcttcgtc gtcggccatc tcgccggcaa cctgcagatc ttcgatgggc
     1501 cggcgcgcct caatgcttac ggcaaattcc tgaagagcct cggcgaactg ttgtggattg
     1561 cccgcggcat tctcatcgtc tccgtggtgc tgcacatctg ggcgacggtc cagcttgcgc
     1621 tgcgcaacat gaaggcgcgc ccggttggct ataaggtgaa gaagtccgtt gcctcgaact
     1681 acgccgaccg caccatgtac tggagcggcc ccatcatcct cgcgttcgtc atcttccacc
     1741 tgcttcagtt cacggcaggc accctgcatc ccggggccgc caccttcagc gacaccgaca
     1801 tctactacaa cgtcgtcgcc ggcttcagcg tctggtgggt ctccgcctgg tacatcctgt
     1861 cgatgatcct tctcggcttt catctgcggc acgggatctg gagcgcgttc cagacccttg
     1921 gggccaatca tccccgtcac acgccggtgc tcaaacaagc cgcgctctgg gtcgcaattc
     1981 tcatcactgt cggttacatt tccatccccg tggccgtgct agccgggtgg gtgaggtaga
     2041 catggccatc aagctcgacg caagaattcc ctccggaccg atcgaacaag cctgggacaa
     2101 cgcgcgcttc gagatgaagc tggtgaaccc ggcgaacaag cggcgccacc atctcatcgt
     2161 cgtcggctct ggactcgcag gcggcgccgc ggccgccacg ctgggcgagc tcggctacaa
     2221 cgtgaagtgc ttctgtttcc aggactcgcc gcggcgcgcg cactccatcg ccgcgcaggg
     2281 gggcatcaac gccgcgaaaa attatcaaaa cgacggcgac agcatctatc gcctgtttta
     2341 cgacacggtc aaaggcggcg acttccgcgc gcgcgaagcc aacgtctatc gcctggccca
     2401 gaacagcgtt catatcatcg accagtgcgt cgcgcaggga gtccccttcg cgcgcgaata
     2461 cggcgggacc ctcgacaacc gctctttcgg cggcgcgcag gtttcgcgca ccttttacgc
     2521 gcgtgggcaa accggccagc agctcctgct cggcgcgtac caggccctcg agcgccaggt
     2581 cgacgccggc caggtcacga tgatcccccg caccgaaatg ctggacctga tcgtgatcga
     2641 tggacaggcg cgcggcatcg tgacccgcaa cctcatcacc ggcaagcttg ccgtgcatct
     2701 ggccgatgcc gtcatcctgg ccaccggtgg ctacggcaac gtctactacc tctcgaccaa
     2761 cgccaaagga tccaacacga cggccatctg gcgcgcgcac aaacatggcg cgctcttcgc
     2821 caacccgtgc ttcacgcaga ttcaccccac ctgtattccc gtcaccggtg attaccagtc
     2881 caagctgacg ctcatgtcgg agtcgctgcg caacgacggc cgcatctggg tgccaaagca
     2941 aaaaggcgac aagcgcaagc ccgatcagat ccccgaagcc gaacgcgact actatctcga
     3001 gcgcaaatac cccgccttcg gcaacctggt cccccgcgac gtcgcatcgc gcaatgccaa
     3061 agcggtgtgc gacgagggcc gcggcgtcgg cgaaaccggc ctcggcgtgt atctcgactt
     3121 cgccgaagcc atcggccgtc tcggggtcaa taccatccgc gagcgctacg gcaatctgtt
     3181 cgacatgtat cagcgcatca ccgatgagga tccctacaag gtccccatgc gcatctatcc
     3241 ggccatccac tacaccatgg gcgggctctg ggtcgactat cagctgcaaa gcacgattcc
     3301 cggcctgttc gtgctcggcg aagccaactt ctccgaccac ggcgccaatc gcctgggcgc
     3361 cagcgccctg atgcaggggc tctccgacgg ctacttcatc atcccctaca cggtcggcaa
     3421 ctacctcgcg actgcaaaac cgaagccggt caagccggac catccggccg cgcaggccgc
     3481 tctcgctgca acggagcaag gcatcgcgaa gctgctcgcc atcaaaggca agcgcaccgt
     3541 cgattcgttc catcgcgagc tcggcaagat catgtgggac gactgcggca tgtcgcgcaa
     3601 cgcgaagggg ctggaggaag ccatcggcaa gattcgcgct ctccgcgaag agtactggca
     3661 gaacgtgaac gtgccgggct ccggcgacga tctgaaccag agcctcgaga aggccggacg
     3721 cgtcgccgat ttcctggagc tgggtgagct gatgtgcatc gacgcgctcg accgcgatga
     3781 atcctgcgga gggcacttcc gcgaggagta ccagacgccg gaaggcgaag cgcagcgcga
     3841 cgacgagcat ttcagctatg tcgccgcctg gggatggacc ggccccggca atcctcctga
     3901 gctcaacaag gagccactgg agttcgctta cgttcacccg agccagagga gctacaagta
     3961 atgaaagtga cactgaccat ctggcggcaa aagaacgcag cggcccaggg caagttcgtc
     4021 agctactccg tcaacgttga aaacggagaa atgtccgtac tggaactgct cgatgtgctc
     4081 aacgaatcgc tgatcgagaa aggcgaagag ccagtcgcct tcgatcacga ttgccgcgaa
     4141 ggcatctgcg gcatgtgcgg cttcatgatc aacggcgtcg cgcacggtcc cctgccagcg
     4201 acgacgattt gccagctcac catgcgtcat ttcaaagatg gcgaagagct gatcctggag
     4261 ccgtggcgct ccgccgcctt cccgttcgtg aaggatctcg ttgtcgatcg ccgctccttc
     4321 gatcgcatca tccaggcggg tggctatgtc tccatctcca ccggcagcgc gcctgatggc
     4381 aacgcgatcc cggtcggtaa agaagacgcg gatcgcgcta tggatgcggc ggcctgcatt
     4441 ggctgcggcg cttgtgtcgc cgcgtgtcct aatgggtcgg ccgcgctctt caccggcgcg
     4501 aagattgccc acctgggaat cctgccgcag ggcaagccgg aagaagacca ccgcgccgta
     4561 cgcatggtgg cgcaaaccaa catcgaaggc ttcggcagct gcaccaatat cggcgaatgc
     4621 accggcgttt gccccaagga aattccgctg gaagtgatcg cgaagatgaa ccgcgattat
     4681 cttgccggcg cctggaaaga gcgcaaagag gtgatccacg cgattgcgcc ggtgcggagt
     4741 tgggagatgg agaagtagcc atccacttcg ggtgcccaag cccgcagtgg gatgggagcc
     4801 ctgtcgcgaa acgcgtacgt gcggcccgca cgctacaatc cccttacgga ttcttcagca
     4861 tgatgcctct gcggccactc gccctcgttg ccctgacttt gtcgattcca ctttcctcca
     4921 gaccgcagcc caccgcaaaa atggcccaca aacgcgctcc ccagaccgca gccgccgttc
     4981 atcgctccgc catcgtc
//
LOCUS       DASZYN010000476         3892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_24420, whole genome shotgun sequence.
ACCESSION   DASZYN010000476 DASZYN010000000
VERSION     DASZYN010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24420"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VHE33_12310"
     CDS             complement(<1..713)
                     /locus_tag="VHE33_12310"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HVT98282.1"
                     /translation="MTRRRWIADTWDEATAFIAGTQATHLIRVLRAQPGMEFDLVAGG
                     RVFHSVLAGITGDAVRFNLVAEVEADPALPVTLFLSVFKFDRMEWAIEKATELGAASI
                     VPMIARRTEKHLAQAASARAERWRRLALEASKQSRRSDVLRIENPIAVKEAVKLNPES
                     VRLVLAEQERSTTLHHALEESLAASGDEIPTICMAAGPEGGWTAEEEALFDAENWKPV
                     SLGPRILRAETAAITAMAVA"
     gene            complement(1094..1834)
                     /locus_tag="VHE33_12315"
     CDS             complement(1094..1834)
                     /locus_tag="VHE33_12315"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HVT98283.1"
                     /translation="MHEIQSSGPPAPPGNFTVRVAPWWHTALIVLLLVAASFLGARSA
                     HRGAVAAHHLVRYSSGILMEWALLLLVWWGLRMKRVRFAELLGFRRGWNALMEDWLAA
                     GVFWIAAMIVLAIVGLSLKAAGLSAPQKTLMSIAPRTPLELLLWMMLSCSAGFCEEFV
                     FRGYLLRQFSSLGAGLWLGVLCSSLLFGISHGYEGAAGMIAITVYGAMFCMLVFLRKS
                     LRPGMIAHAWHDIFSGLTLALLRHFHLL"
     gene            1911..3098
                     /locus_tag="VHE33_12320"
     CDS             1911..3098
                     /locus_tag="VHE33_12320"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HVT98284.1"
                     /translation="MDPIVIVSGVRTPVGKFQGSLTDVTATRLGAVVVREAVKRAGLQ
                     PEQVDECIMGNVVSAGLGQNPARQAALYGGLPPEVSAVTVNMVCGSGLRAVALGAQSI
                     QTGNSEIVVAGGMESMTNAPYLLPQARSGFRMGNATAVDAMVNDGLWDVYNDFHMGQT
                     AELVAEKYGIGREEQDCYALLSHKKAAAAWREGRFAAEVVPVEIPAKKKGQAPTLFER
                     DESVREDASIEALRALKPAFKKDGSVTAGNAPGVNDAAAAVVVMSAAKAGELGLTPMA
                     RIVSHATSGIDPKWVMMAPVNGARKAAERAGWSFESIDLFEFNEAFSVQSLAIMRELG
                     VPQEKVNVNGGSVAIGHPIGASGARVLVTLMYEMVQRKARRGLVALCLGGGNSVAMCI
                     ERD"
     gene            complement(3550..>3892)
                     /locus_tag="VHE33_12325"
     CDS             complement(3550..>3892)
                     /locus_tag="VHE33_12325"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HVT98285.1"
                     /translation="EVGVACSMAAGGLAAALGGTNDQVEHAAEIAMEHNLGMTCDPIG
                     GLVQIPCIERNAMGAVKAVQACRIAMNETEGHKVSLDQVIRTMYQTGLDMQSRYKETS
                     LAGLALNVIEC"
BASE COUNT          751 a         1077 c         1330 g          734 t
ORIGIN      
        1 gcgacggcca tggcggtgat ggcggcggtt tctgcgcgga ggatgcgggg gccgaggctg
       61 accggcttcc agttttccgc gtcgaacagg gcttcttctt cggcggtcca tccgccttcc
      121 ggtcctgctg ccatgcaaat ggttgggatc tcgtcgcctg aagcggcgag gctttcttcg
      181 agcgcgtgat ggagggtagt cgagcgctcc tgctcggcga gcaccaggcg cacggactcg
      241 ggattcagct tcacggcttc tttgacggcg atggggttct cgatgcgcag cacgtcggag
      301 cgacgcgact gcttcgaggc ttcgagcgcg aggcgacgcc atcgttcggc gcgggccgag
      361 gcggcctggg ccagatgctt ttcggtgcgg cgcgcgatca tgggcacgat gctcgcggcg
      421 ccgagctcgg tggccttctc gatggcccac tccatgcggt cgaacttgaa gacagaaagg
      481 aacagcgtga cgggcagcgc gggatcggcc tcgacttcag cgacgagatt gaatcgaacg
      541 gcatctccag tgatgccggc gagcacgctg tggaagacgc gtccgccggc gaccagatcg
      601 aactccatac caggctgggc gcggaggaca cggataagat gagtggcctg ggtgccggcg
      661 atgaaagcgg tggcttcatc ccaggtgtcg gcaatccatc tgcggcgcgt cattccttca
      721 gcgtacagcg gctggtgctg gaggattgga gaaacagcga gagacggcgc gatgtttcga
      781 tcgcctgaaa aagcgggccc aaaagcatcg gggggacgct catcgcgtcc ccccgaggtg
      841 ttgccgatgt cctcttactt tttcttcgag gacttcttgg ctgccttctt cgcgctcttc
      901 ttagctgcct tttttgctgc catggtttct attctccctt gttcgatgtc tacatcgaag
      961 tctgcaacat aaaatgctgc agttaacgaa tgtatagagt tcctcgcatg gagtgtcaag
     1021 gaaaaaattc aaattttgtc tacaactttt ctctctccgc gttgcgatct cactcgcatg
     1081 atgcgaacga aaatcacagc agatgaaaat ggcggagcaa cgcgagcgtc aatccactga
     1141 aaatgtcgtg ccatgcatgc gcgatcatgc ccggacgaag actcttccgc agaaacacca
     1201 acatgcaaaa catcgcgccg tacacggtga tggcgatcat tccagccgct ccttcgtaac
     1261 cgtggctgat accgaagagg agtgaagagc agagaacgcc gagccataat cccgcgccga
     1321 gcgaggagaa ctggcggagc agatagccgc gaaagacgaa ctcttcgcag aatccggccg
     1381 aacagctgag catcatccaa agcaacagtt cgaggggcgt gcggggcgca atggacatga
     1441 gcgtcttctg cggagcggac aatccggcgg ccttcagtga caatccgacg atggcgagga
     1501 cgatcatcgc ggcgatccag aacacgcctg cggcgagcca atcctccatc agcgcgttcc
     1561 acccgcggcg gaatccgagg agctctgcaa agcgaacgcg cttcatgcgc agcccccacc
     1621 agacgagcag gagcagcgcc cattccatga ggattccgga actgtagcgg acgaggtggt
     1681 gtgcggccac cgcaccccga tgcgccgagc gcgcccccag gaaagaagct gcgacgagca
     1741 gcagcacgat gagcgcggta tgccaccatg gcgcgacgcg gacggtgaaa ttgccgggcg
     1801 gggcgggcgg gccactggac tggatctcgt gcatctcacc ctttctgtct ggtatcttcc
     1861 tctggattct actcaggggt ctcgcgcatc gaatgagtca agggagaacc atggatccga
     1921 ttgtgatcgt ctccggcgta cgcacgccgg tcgggaaatt tcagggatcg ctgactgacg
     1981 tcaccgccac gcggctgggc gcggttgtgg ttcgagaagc ggtgaaacgc gcggggctgc
     2041 agccggagca ggtagatgag tgcatcatgg gcaacgtggt gtccgccggg ctgggacaga
     2101 accctgcgcg gcaggcggca ctgtacggcg ggctgcctcc ggaagtcagc gcggtgacgg
     2161 tgaacatggt ttgcggttcg gggctgcgcg cggtggcgct gggtgcgcag tcgatccaaa
     2221 ccggcaactc ggagatcgtg gttgcgggcg ggatggaatc gatgaccaac gcgccctatc
     2281 tgcttccaca ggcgcgcagc ggcttccgca tgggcaacgc gaccgccgtg gacgcgatgg
     2341 tgaacgacgg cctgtgggat gtgtacaacg atttccatat gggacagacg gcggagctgg
     2401 tggcggagaa gtatggcatc ggccgggaag agcaggattg ctacgcgctg ctgtcgcaca
     2461 agaaggcggc ggccgcgtgg cgcgaggggc gcttcgctgc ggaggttgtg ccggttgaga
     2521 ttccggcaaa gaagaagggg caggcgccga cgctgttcga gcgggatgag tcggttcgcg
     2581 aggatgccag catcgaagcg ctccgcgcgc tgaagccggc attcaagaag gacggcagtg
     2641 ttaccgcagg caatgcgccg ggcgtaaacg atgcggcggc ggcggtcgtg gttatgtcag
     2701 ccgcgaaggc tggagaactg gggctgacgc cgatggcgcg gatcgtctcc cacgcgacga
     2761 gcgggatcga tcccaagtgg gtgatgatgg caccggtgaa cggagcgcgc aaagcggcgg
     2821 aacgcgcggg ctggagcttc gagtcgatcg atctgtttga gttcaacgag gcgttctcgg
     2881 tgcagtcgct ggcgatcatg cgggaactgg gagtgccgca ggaaaaggtc aacgtgaacg
     2941 gcgggtcggt ggcgatcgga catccgattg gcgctagcgg cgcacgcgtg ctggtgacgc
     3001 tgatgtatga gatggtgcag cggaaggcgc ggcgggggct ggtggcgctt tgcctgggtg
     3061 gggggaattc ggtggcgatg tgcatcgagc gcgactgatc agcgtccctg acagcctggg
     3121 ttcagcctga aggttcttcc aggtgacgag tctttcgcgg gttgtgaaac tcgtctgctt
     3181 gggcgactcg ctcaggacag gcttaaggca tctaccgtcg tgccgctggc gtggcaagct
     3241 tcgcgggaaa gaccgaatca tccggtcgaa gcacgagtgt ggtgtttccg aagtttcttt
     3301 atgcggagag aagcaggtcc ctccgcttcc ccttcgctac gctcagggtc cggtcgggat
     3361 gacaaggtaa agaaactatc gaacagggcg gaaaaagcag gttcctcggc tcaccacccc
     3421 caaactgaga tacgtttggg gcccccttcg ctcggaatga caagtcactc ggggcccttg
     3481 gtgcgctggg gccccgttca ctcagaatga cggaccgttg attttgcagc gaatgtgttg
     3541 gccgccacac tagcattcga tgacgttgag ggcgagaccg gctaatgacg tctctttata
     3601 gcgggattgc atgtcgagac cggtctggta catggtgcgg atcacctgat cgagcgagac
     3661 tttgtgtcct tcggtttcgt tcatggcgat gcggcaggcc tggacggcct tgactgctcc
     3721 catggcgttg cgctcgatgc agggaatctg cacgaggccg ccgatggggt cgcaggtcat
     3781 gccgagattg tgctccatgg cgatttccgc tgcatgctcc acctgatcat tggtgcctcc
     3841 gagagcagcc gcgagtccgc cggcggccat ggaacaggca acgccgacct cg
//
LOCUS       DASZYN010000477         3240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_986170, whole genome shotgun sequence.
ACCESSION   DASZYN010000477 DASZYN010000000
VERSION     DASZYN010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986170"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VHE33_12330"
     CDS             complement(<1..324)
                     /locus_tag="VHE33_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98286.1"
                     /translation="MTEGTRIEPGQTLLIDADDTLWENNIYFERAIAAFISYVDHKEY
                     SAEQVREKLNEVERENVRLHGYGLVSFRRALVTCFERLSTGPITPEKHERIMSFAQSI
                     ADQEIE"
     gene            495..1544
                     /locus_tag="VHE33_12335"
     CDS             495..1544
                     /locus_tag="VHE33_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98287.1"
                     /translation="MRLYSPTRCTLKILAVLACAIFACSSARADVSLLLEEPFGRFGA
                     MNPTGHAAVWLDRVCADTPTHLRPCEAGERGVVLSRYHRVDGYDWLAMPLLAYLYAVD
                     DPSEVPESATPKLELKLRDAYRREHLADLVPDDPEHEIPGGDWIQLVGASYDRRIWGF
                     QVATTREQDAALIARFNHRRNISHFNLFFNNCANFSQKILNFYYPHAIHRNFIADLGM
                     MTPKQAAKSLVKYSRHHPETDLYAFVIPQVPGTIDRSTKVNGVIESLVKSKKYVLPLT
                     YLNPWVTGGLVVAYFGGGRFHPDHHAAIFDPTQDEVPGVMMRTTSSAETIGSSDAAPS
                     PAPMRDVSSDELPRP"
     gene            1671..>3240
                     /locus_tag="VHE33_12340"
     CDS             1671..>3240
                     /locus_tag="VHE33_12340"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HVT98288.1"
                     /translation="MRQPWLAAACLSIALTLTCAAQSSAPAAGEQQYQPTPGYDATAM
                     DTSANPCEDFYQYACGNFAKLHPIPPDMPMFDQFVNLFEFNTAALHGLVEKAADAHAA
                     PGTNEQKIGDYYSSCMNTDAIDKLGLAPLQPDLARIAGLQSKQALPALIAHLNRESVG
                     VFLDYGSQQDFKDASREIAYIDQSGLGLPEKDYYLRTDAKSEALRKEYVAHLTNVLKL
                     LGDAPETAQSEAQKILDFETALAKASQGVVERRDPNKIYHMMALTSFSATAPVLDATK
                     YVQMMGSPAITELNVVSPDFFPALNKAIDDTDLDTLKAYMRVRLADSLSMRLPQAFDQ
                     EHFDFYGRKLTGTPEQEARWKRCVQATDSALGEALGQLYVAKYFTPDMKAATLREVHD
                     IEAAMGKDIDQIDWMSPETKVKAKEKLQAVADKIGYPDKWRDYSKLEMKPDDALGNSL
                     RSREFEVAYQLNKIGKPVNRQEWQMTPPTVNAYYDPSMNDINFPAGILLPAFYDKDAT
                     DATNYGHIGAVVGHE"
BASE COUNT          643 a         1037 c          948 g          612 t
ORIGIN      
        1 ctcgatctcc tgatcggcga tcgactgcgc gaagctcatg atgcgctcgt gcttctccgg
       61 cgtgatcggc cccgtcgaca gcctctcaaa gcaggtaacc agcgcgcggc gaaaactcac
      121 cagcccgtag ccgtgcagcc gcacgttctc ccgctccacc tcgttgagtt tttcccggac
      181 ctgctcggcg gaatactcct tgtgatccac gtaagagatg aacgctgcga tggcgcgctc
      241 gaagtagatg ttgttctccc agagcgtgtc gtctgcatcg atcagcaggg tctggccagg
      301 ctcgattcga gtgccttcag tcatcctgtc cctcaggcta ccagcaacgg aacctgcatc
      361 taaccgagcg gcgcggtggt gccgatttgc gtaccacaaa ggtgttaaac cgcagcgcaa
      421 cactttgtta aatagcgggt tatgatcagg tatccacctg aaatttgtgc tttggttcgg
      481 cctctcgata acggatgcga ctctactccc caacacgctg cacgcttaag atcctggcgg
      541 tcctcgcctg cgccattttc gcctgttctt cggctcgcgc ggacgtctcc ctgctgctgg
      601 aggagccttt tggccgtttc ggcgccatga atcccaccgg ccatgcggcc gtctggctcg
      661 accgcgtgtg cgcggacaca cccactcacc tgcgtccctg cgaagcggga gaacgcggag
      721 tcgtcctcag ccgctaccac cgcgtggatg gctacgactg gctcgcaatg cccctgctcg
      781 cgtatctcta cgccgtcgac gacccgtctg aggttcccga aagcgcgaca ccgaagctgg
      841 agctgaagct gcgcgatgcc tatcgccggg agcatctggc agatctcgta ccggacgacc
      901 ctgagcatga gattccgggc ggagactgga tccagctggt gggcgcgtcg tacgaccgcc
      961 gcatctgggg gttccaggtc gccaccacgc gcgaacagga cgcagccctg attgcgcgct
     1021 tcaaccaccg ccgcaatatt tcgcatttca atctgttctt caacaactgc gccaattttt
     1081 cgcagaagat cctcaacttc tactatccgc acgccattca ccggaacttc atcgccgacc
     1141 tgggcatgat gacgccgaag caggcggcca agtcgctggt caagtacagc cggcatcatc
     1201 ccgagaccga tctgtacgcc tttgtcattc cgcaagtgcc cggcaccatc gaccgcagca
     1261 cgaaggtgaa tggcgtcatt gagtcgctgg tgaaatcgaa aaaatacgtg ttgccgctga
     1321 cgtatctcaa cccgtgggtg acggggggcc tggtggtggc gtatttcggc ggcgggcggt
     1381 tccatcccga tcaccatgcc gcaatctttg acccaaccca ggatgaggtc ccgggggtga
     1441 tgatgcgtac gacgtcgtca gccgaaacga tcggttcgag cgacgctgcc ccgtcgcctg
     1501 ccccaatgcg cgacgtgtcg agcgacgaat tgccgcgccc ctgaggctcg cgcctttcgc
     1561 tcgtctccgt tcgaggtcca cccaccacaa atcctgcccg cgaccctctc ctcctgaggg
     1621 cggaggccgc tccagtcgcg tatcatcagt agtttgcctc aggaggattc atgcgccaac
     1681 cctggctggc tgccgcctgt ctgtcgatcg ccctcaccct tacctgcgcc gcgcagtcca
     1741 gcgcacctgc tgccggtgag cagcaatacc agccgactcc gggctacgac gcgacggcca
     1801 tggatacctc cgccaatccg tgtgaggact tctatcagta cgcctgcggc aacttcgcaa
     1861 agctgcaccc gattccgccg gacatgccga tgttcgatca gtttgtgaat ctgttcgagt
     1921 tcaataccgc ggcgctgcac ggactggtag agaaggcggc cgacgcccac gccgcccccg
     1981 gcacgaacga gcagaagatc ggcgattact actccagctg catgaatacc gatgccatcg
     2041 ataagctcgg cctggcgccc ctgcagccgg atctggcgcg cattgcagga ctgcaatcga
     2101 agcaggcgct gcccgcgctc atcgcacacc tcaatcgcga gtcggtaggt gtctttctcg
     2161 attacggttc gcagcaggat ttcaaggatg cttcgcgcga aatcgcctac atcgaccaga
     2221 gcggtctggg cttgccggag aaggattact acctgcgcac cgacgcgaaa agcgaggcgc
     2281 tgcgcaagga gtatgtcgcg catttgacca atgtgctcaa gctgctgggc gatgctccgg
     2341 agaccgcgca gagcgaggcg cagaagatcc tggatttcga aactgcgctg gccaaagctt
     2401 cccagggcgt cgtggagcgg cgcgatccga acaagattta ccacatgatg gcgctgacca
     2461 gcttttccgc gacggccccg gtgctggacg ccacgaagta cgtgcagatg atgggctcac
     2521 ccgcgatcac agaactgaac gtggtctcac cggacttctt ccccgcgctc aacaaagcca
     2581 ttgacgatac ggatctggac acgctgaagg cctacatgcg cgtgcgcctg gcggactcgc
     2641 tttccatgcg cctgccgcag gccttcgatc aggagcactt cgacttctac gggcgcaagc
     2701 tgaccggcac gcccgagcag gaggcgcgct ggaagcgctg cgtgcaggcg actgattcgg
     2761 cgctgggcga agccctgggc cagctgtacg tggcgaaata tttcactccc gacatgaagg
     2821 ccgcaacgct gcgcgaggtc cacgacattg aagccgccat gggcaaggac atcgaccaga
     2881 tcgactggat gagtccggaa acgaaggtca aggcgaagga aaagctgcag gccgtggccg
     2941 acaagatcgg gtatccggac aagtggcgcg actactcgaa gctggagatg aagccggacg
     3001 acgcgctggg gaattcgctg cggtcgcggg aatttgaggt cgcctaccag ctgaacaaga
     3061 tcggcaagcc ggtcaaccgc caggagtggc agatgacgcc gccgacggtg aacgcatact
     3121 acgatccgtc gatgaacgac atcaacttcc ctgccggcat tctgctgccc gcgttctacg
     3181 acaaggatgc aaccgatgcg acgaactatg gacacatcgg agccgttgtc gggcatgagc
//
LOCUS       DASZYN010000478         3220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1241379, whole genome shotgun sequence.
ACCESSION   DASZYN010000478 DASZYN010000000
VERSION     DASZYN010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241379"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VHE33_12345"
     CDS             complement(<1..668)
                     /locus_tag="VHE33_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98289.1"
                     /translation="MKWFLISGLCALLAVTLAVVPGTAAQNGSSRIFVNVVLVQLNVA
                     VTDKKGNYVTGLKPENFLVTEDRIPEKISSFEEGGAPGGILEAGTDPAPENGGGSAPV
                     GTNTAGQQQTAPKPAETSAVQLGLPKASHLAGASVFVLFDTSDYMYRGFVFAQDAIGG
                     FVRTMEEANKIAFYSYSRNLSRATLLTTDRSVVMRGVRNTVAGDEAALYNALLLTVRD
                     AAQL"
     gene            complement(671..>3220)
                     /locus_tag="VHE33_12350"
     CDS             complement(671..>3220)
                     /locus_tag="VHE33_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98290.1"
                     /translation="WHQKSGTNLLDALLHDRQLDTLYWALSKNDEGTRSALRERRGLR
                     WMLPIAPALDFYGSQLSIRDGHVLVPGGPQADHAWQELVGANPSNPDDFVEHLYSRDR
                     GWMGAYYDALSRVGRTQQAHLTEGSRLKQLYEVYRRASNNLYATRGVFARNADLLILF
                     SRLQWQPDGTPYIPGGLATWKDVFSQDSTPKMVRQWVRSARTWDSPEQLLMTLVACAN
                     FPTEAGPLQMYLTVSSIDAARPANAHLDQGTVRLIAQKFSEYHSWYLVFSEFPELNDT
                     SIVQFVKAADGVTAINNPALRANALGALQANVGLWEILARQQQIPPAAMNQSWQDAVH
                     PFIGASTSVQLFDAARASLRATLNAAAGQGNLTEDQIIELMAGPAQRADTGRRVHGQI
                     ADRIRSVLDDQRLVSLDTLFSLYDGIDQLAHGSAVKDQLIPLAANLKEFEMPRAIFSE
                     GEKAAWAPEIYSSRHAELQVRTDLTKQIQGASTPAQLEVARGELLPFLRDTLVGLNYA
                     YYEPPGAQVLHNNPLFVRSHDFSGNSIQGYGHIWNSPELVGIGVTAGGGAYLIGSLAN
                     LPYALATAEEDFIAPEHVQALIWQAAGPAILVDAIEPRWWDVSPSELHAAALYQRAGE
                     DLLRAAPGNAELRGRLVNILSDLMSPRRLELTQHAMLQTADITRLMPGITPAEKFYLA
                     TEYRKAYPSEAASFGQASQELDALIHKSPEDGSAARLSKDFGVPHPTLEQTNACAILT
                     LKPLPAYSGEAYALMGESWESSNLYWARLADEMGYAPESLNILIPELSRRMIAKIFAT
                     DLEDWPAILRAMEQTGEEFREGGIRIAGASPIPTNTTSSAQALPLSSSTEQ"
BASE COUNT          604 a         1127 c          891 g          598 t
ORIGIN      
        1 tgcagctgcg ccgcatcacg caccgtcagc agcagcgcgt tgtacagcgc cgcctcgtcg
       61 ccggccaccg tgttgcgcac cccccgcatg accacggatc ggtcggtcgt cagcagcgtc
      121 gctcgcgaca aattccggct gtaagaatag aacgcgatct tgttggcctc ttccatggtg
      181 cgcacgaagc ccccgatcgc atcctgcgca aaaacaaagc cgcgatacat atagtcgctg
      241 gtgtcgaaca gaacaaacac gctggcgccc gcaagatgcg aggctttggg cagtcccagc
      301 tgcacggccg aagtctcagc cggcttgggc gcggtctgtt gctgccccgc cgtgttcgtc
      361 cccaccggag cactgccacc gccattctca ggagcaggat ccgtgcccgc ttccaggatc
      421 ccgcccggcg cgccgccctc ctcaaacgag gaaatcttct ccgggatcct gtcctcggtc
      481 acaaggaaat tctccggctt cagccccgtg acgtagttcc ctttcttatc ggtcacagcc
      541 acgttcagct gcaccagcac gacgttgacg aaaatgcgcg acgagccgtt ctgggccgcc
      601 gtgcctggaa cgaccgcgag cgttaccgca agaagcgcac acaaaccgga aatcaggaac
      661 catttcatcg tcactgctcc gtggaagaac tcaggggcag cgcctgcgcg ctgctcgtgg
      721 tgttggtcgg aatcggactc gcacccgcaa tccggatgcc cccctcgcgg aattcctcgc
      781 ccgtctgctc catggctcgc agaatcgccg gccagtcctc aaggtcggtg gcgaaaattt
      841 tcgcaatcat gcgccggctc agttccggaa tcagaatgtt cagcgactcc ggtgcgtagc
      901 ccatctcatc cgccaggcgt gcccagtaga gattgctcga ttcccacgat tcgcccatca
      961 acgcatacgc ttcgccggaa tacgccggca gcggcttgag cgtcagaatg gcgcacgcgt
     1021 tcgtttgttc caaagtcgga tgcggcaccc cgaaatcctt cgagagacgc gcggcgctcc
     1081 catcctcagg gctcttgtgg atcagcgcgt ccagctcctg gctcgcctgc ccaaacgaag
     1141 ccgcctcgct cggatacgcc ttgcgatatt ccgtcgccag gtagaacttc tccgccggag
     1201 tgatgccggg catcagcctc gtaatgtccg ccgtctgcag catcgcgtgc tgtgtcagct
     1261 ccaaacggcg tgggctcatc agatcactca gaatgttcac cagccggccg cgcaactccg
     1321 cgttgccggg cgcggctcgc aacaaatcct cacccgcccg ctggtacagc gctgccgcgt
     1381 gcagttcgct ggggctcaca tcccaccagc gtggttcgat cgcatccacc agaatggccg
     1441 gccccgccgc ctgccagatc agcgcctgca cgtgttccgg cgcgataaag tcctcttccg
     1501 ccgtcgccag cgcatacggc agattcgcca gagagccgat caggtaagcg cctccaccgg
     1561 ccgtcacacc aatcccgacc agttcaggcg aattccagat gtgcccgtag ccctggatcg
     1621 aattgcccga gaaatcatgc gagcgcacga acagcggatt gttgtgcagc acctgagctc
     1681 ccggcggctc gtagtacgcg tagttcagcc ccaccagcgt gtcccgcagg aacggcagca
     1741 gttcgccccg cgccacctcc agttgtgccg gcgtgcttgc cccctggatc tgcttcgtca
     1801 gatccgtgcg cacctgcagt tctgcgtgac ggctgctgta aatctccggt gcccacgccg
     1861 ctttctcacc ctcgctgaag atggcgcgcg gcatctcgaa ttccttcaga ttcgcggcca
     1921 gcggaatcag ctgatccttt accgccgacc cgtgtgccag ctgatcgatg ccgtcataca
     1981 gcgaaaacag cgtatccagc gataccagcc gctgatcgtc cagcaccgag cgaatccgat
     2041 cggcgatctg tccatgcacc cggcgcccgg tgtccgcgcg ctgcgcagga cccgccatga
     2101 gctcgatgat ttgatcttca gtgagatttc cctgcccggc cgcggcgttc agcgtcgcgc
     2161 gcaacgaagc gcgcgccgcg tcaaataact gcacggatgt ggaagccccg atgaacggat
     2221 gaaccgcatc ctgccacgac tgattcatcg ccgccggcgg aatctgctgc tggcgcgcca
     2281 ggatctccca caaacccacg tttgcctgca gcgcacccag cgcattcgcc cgcagcgccg
     2341 ggttattgat cgccgtcacc ccatcggcag ccttcacgaa ctgcacgatc gacgtgtcat
     2401 tcagctcagg aaattccgag aacaccagat accagctgtg atactccgag aacttctgtg
     2461 caatcagccg aaccgtaccc tgatccaggt gtgcgttcgc cggccgcgcc gcatcgatcg
     2521 agctcaccgt cagatacatc tgcagaggcc cggcttccgt gggaaagttc gcgcaggcca
     2581 caagcgtcat gagcaattgc tcgggactgt cccacgtccg tgcgctgcgc acccactggc
     2641 gaaccatctt cggcgtcgaa tcctggctga atacgtcctt ccacgtcgcc agtccgcccg
     2701 gaatgtaagg cgtcccgtcc ggctgccatt gcaggcggct gaacaggatc agaagatccg
     2761 cgttccgcgc gaaaacgccg cgcgttgcat acagattgtt cgaagctcgc cggtagacct
     2821 catagagttg cttcagccgc gagccttctg tcagatgagc ctgttgcgtg cgacccaccc
     2881 gcgagagcgc atcatagtag gcccccatcc atccgcgatc ccgcgaatac aaatgctcga
     2941 cgaagtcatc gggattcgat ggattggcgc ccaccagttc ctgccatgca tggtccgcct
     3001 gcggtccgcc cggaaccagc acatgtccgt cgcgaatcga aagctggctt ccgtaaaaat
     3061 ccagcgctgg tgcgatgggc agcatccacc gcaggccgcg ccgctctcgc agagccgagc
     3121 gtgtcccctc atcgttcttc gacagcgccc agtacagcgt atccagctgg cggtcgtgca
     3181 gcagcgcgtc cagcagattc gtcccgctct tctggtgcca
//
LOCUS       DASZYN010000479         9996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_275842, whole genome shotgun sequence.
ACCESSION   DASZYN010000479 DASZYN010000000
VERSION     DASZYN010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 9996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9996
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275842"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VHE33_12355"
     CDS             complement(<1..662)
                     /locus_tag="VHE33_12355"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HVT98291.1"
                     /translation="MHASNDIPFLDLVTPHVELAEELTAVFRRALFTAGFVGGPMVED
                     FEKAFAAFCGTTHSVAVNSGTDALRFAYTACGIVPGDVVVTVPNTFIATTESISQAGG
                     IPEFVDMDKNTYNMSIERLQEFLEKGCTRDASGKLISKRHQRPVTAIVPVHLYGQMAD
                     MDSILRLASEYGLQVIEDACQAHGAEYFSGKLNRWMKAGTMGHAAAFSFYPGKNLGAL
                     GEG"
     gene            complement(649..1158)
                     /locus_tag="VHE33_12360"
     CDS             complement(649..1158)
                     /locus_tag="VHE33_12360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HVT98292.1"
                     /translation="MSTFNCIADDVKLGTNVRLSKFINLYGCEVGDDTKIGAFVEIQK
                     NATVGKRCKISSHTFVCEGVVIEDNVFIGHGVTFINDSYPRATAADGQLQTEADWKVE
                     RTVIRRGASIGSGATVLSNVTVGENAIVGAGSVVTKDVPPNTIVAGNPARILRSMEES
                     KEDPHHARV"
     gene            complement(1155..2153)
                     /locus_tag="VHE33_12365"
     CDS             complement(1155..2153)
                     /locus_tag="VHE33_12365"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HVT98293.1"
                     /translation="MNFGVIGYGYWGPNVVRNLASLEDSQVAAIAELSPAAQKRAHKA
                     YPGIHITSDAMEVITSPNIDAIAVITPVWTHYELAKAALENGKHVFVEKPFTSNVAQG
                     EKLIDIALQKNLRIMVDHTFLFTGAVRKISQLLREGALGKLYYYDSTRVNLGLFQHDI
                     NVLWDLAPHDLSIMDHVIEGSPEAVVATGQCHLNSHEDVAFMTLYFPENVIAHINVNW
                     LSPVKVRTTLIGGEKRMLVWNDLENDEKVKVYDKGVNINNSEGLYKLLVNYRSGDMWS
                     PQLEQVEALRQELSYFVDCISTGKEAFNDGCAGLRVVKMLEAASESISKRGSLVYL"
     gene            complement(2276..3421)
                     /locus_tag="VHE33_12370"
     CDS             complement(2276..3421)
                     /locus_tag="VHE33_12370"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HVT98294.1"
                     /translation="MTLPRMNKITDIVDPIFDTTASVDRDVLAEEAFRRRIAIERKRT
                     ERSREPFLLMLLEATGPDTPEGSDKTLTGIVAAMQSSTRETDVVGWYKNRSTVGVIFT
                     GLVISDKNSVLSTILGRVSTALQQEVTLERFNQITFSFHFFPDDWESDSSGRPSDPIL
                     YPDLFIPNNNQRVLLRVKRAMDVAGASLMLIACLPLFAIIALAVKLTSKGPVLFRQDR
                     IGQYGRRFTFLKFRSMKVNNDTSEHREYVKQLIAGVAQGVQTANGQQVFKLAGDKRIT
                     PIGKFLRKTSLDELPQFFNVLRGDMSLVGPRPPLPYELAAYQTWHRRRVLQVKPGITG
                     LWQVTGRSRVRFDDMVRLDLRYAISWSPWLDCKILLRTPAAVIKGAY"
     gene            complement(3618..4637)
                     /locus_tag="VHE33_12375"
     CDS             complement(3618..4637)
                     /locus_tag="VHE33_12375"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HVT98295.1"
                     /translation="MSRIFDALQRSESERSGDKGPAAADATELLRHAEKRALSDFAEN
                     ATPEYEVAAPEKPEKPEKTVARNGRGRSSNGASGAAHGSRSSAAAAAEPALAGEHPAI
                     FDQFQMLRVSVPAQGRLVSISDRDSAAAEAFRLLAVRLRNLRRDRPLKKVLVTSSIPQ
                     EGKSMCSANLACSLALRAPQRTLLLEGDIRRPSMAPMFGLAPRPGLSEFLREDRELKD
                     SIYLLEGPGLWFMTAGSAPSNSLELLQSAKLSRLLDQLGELFDWIVIDSPPVLPLADT
                     SVWSRLCDGVLLVTRRGVSEKREMLRAVDALESQKLIGAILNSSNNTPHADYYYNSRP
                     GKGSS"
     gene            complement(4621..5607)
                     /locus_tag="VHE33_12380"
     CDS             complement(4621..5607)
                     /locus_tag="VHE33_12380"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVT98296.1"
                     /translation="MYKAFFKLARNPFDLTPDPTYFVSTRRHNEALAALHYGIRRHKG
                     FVVVTGEVGTGKTLLLRCLLRLLKESKDIAYAYLFNSRLSPTEFLQYILADFGLPASG
                     KNKSELLLDLGQFLVSRGSKGLTTVLIIDEAHHLSEELLEEVRLLSNLETTDDKLLQI
                     VLVGQPELDEKLDSVGLRQLKQRIALRAQLTALDAEETRDYIAHRLQVAGAEGDPKRI
                     FPADAVAAVYRHSRGFPRLINTICENALITAYARQVPAITADIVDDVAREFRLDSEFS
                     EPGGSGTQNEMDVQQAAKVLRDLYSALRKPTARSAELNLPVSLETSEYEPNI"
     gene            complement(5620..7191)
                     /locus_tag="VHE33_12385"
     CDS             complement(5620..7191)
                     /locus_tag="VHE33_12385"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HVT98297.1"
                     /translation="MSEVFEEEKASKFDLGRYLDIGRRRHMYFLIPLLAGWLLVWAVS
                     WFLPPRYKSSTQILVQEPSLPRNVADAAADQSALQARLQTMQQEILSRTRLLTIINGM
                     HLYDDGGKPQSDDDKVATMTKAIDIDLVRDTHNSVTGFKISYSSDNARVAQAVTGQLA
                     ELFINENQKNQYQQSADTTRFLKSQLDQAAQTLADQEAKVKNFQATHIGALPDSVQSN
                     LAILGGLQSQLNNEQDGLNNANQQRTLHEAEIEQYRANPVVAVRSGEVLDPNSVQAID
                     IQLAKLRDQLTDLTSRYTDSYPDVVKLKAQIAQVEQQRKQAMASPREKGEKTTDSLTV
                     AQLQAQLQADNLAIQNRQKAIDSLKVRINEYEARINATPSSVQGLADLQRGYNQSQAN
                     YNDILKKMQESQMATNMEQMQQGERFQLLDPPSLPAKPDFPNRLLFCGVGLAVGLVFG
                     AASVAGFEFMDDRLHSESEISDLLPVPVICEIPTVSNAIDEQVNRRKMLIGWATATVV
                     AMVILAGSAISYLHG"
     gene            complement(7194..8570)
                     /locus_tag="VHE33_12390"
     CDS             complement(7194..8570)
                     /locus_tag="VHE33_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98298.1"
                     /translation="MTPRIVLLLALCAAIPAWSQATGTGTDNPGTSTGTDEQPDNPLA
                     NDGMLTPPPVSGQTYPILTGSEERRNFMTLGATANVAYDDNVEGGYTGVSKGDMIYSL
                     WPTISVNRSSTRLREVLNYSPGFTFYQPSTVFNAMDHNLTADLQYQMGTHTVLDLSDN
                     FDRSSSLFNQPFGTSQGISGGVPTQTSGVLPPFADRLMNTAAAQLTNQTGTNGMIGLS
                     ANFSNLDYPDNRQVTGLYNATSYQGGAFVTQRLSRRQYLGANAQHGKVVSYVNGVDNV
                     MKQDNIFGFYTIYLRALPQSTFSISITAGPEHYSIIQYPEALLSQWTPAGTVSLGWQA
                     HHSSASASITRAVTGGGGLPGAYEEDGAEIQFRRQLRPTWDVSFSGGYTQNKSATPQF
                     AFVVPGGHTLAAGVSASHMLTKNLKIDVGYNWMDQVYKAVVSLSPIPISNREFCAVSY
                     HFTRPLGR"
     gene            complement(8579..9238)
                     /locus_tag="VHE33_12395"
     CDS             complement(8579..9238)
                     /locus_tag="VHE33_12395"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HVT98299.1"
                     /translation="MKLNTVYALVTGSVLFFAFAALTPAQTVASSNSTPDSPSAPEAS
                     SDQVTPSPLNHAHNSTYVIGDDDMIEIDVWKETELSKQLTVRSDGKISMPLVGEIQAA
                     GKTPVQLEDEITTKLRSFITEPAVTVMVLKINSLKFNVMGEVARPGSYSLAARTTIVD
                     AIATAGGFRDFAKKKSIYVLHQKPDGTEARLSFNYSAFIKGKNPKQNILLQSGDTVVV
                     P"
     gene            complement(9369..>9996)
                     /locus_tag="VHE33_12400"
     CDS             complement(9369..>9996)
                     /locus_tag="VHE33_12400"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HVT98300.1"
                     /translation="GANKMPGAANTEDLADAPAWWAVYTQHQHEKTVAEVLAGKGFDV
                     FLPLHETLRRWKDRQKLISLPLFPGYVFVRGILSRRLQVVSTPGIHMILSRGEKIEVV
                     PEPEIDAIRRAVQSSYRVEPHPFLKIGERVRVTRGSLEGVEGILVRKKNLYRLVLSVE
                     MLAQSVGVEVAATDVEPVGAANPKSSPGGIVETGIGDFQKNFPFAGPN"
BASE COUNT         1886 a         3274 c         2749 g         2087 t
ORIGIN      
        1 ccttcgccca gcgcgccaag attcttgccc gggtagaagc tgaatgccgc ggcatgaccc
       61 atcgtgcccg ccttcatcca gcgattcagc ttgccggaga agtactccgc accgtgcgcc
      121 tggcaggcat cttcgatcac ctgcagcccg tactcgctcg ccagccgcag aatcgaatcc
      181 atgtccgcca tctgcccata gaggtgcacc ggcacgatcg ccgtcaccgg ccgctggtgc
      241 cgcttgctga tcagtttccc cgaagcgtcg cgcgtgcagc ccttctccag gaactcctgc
      301 agccgctcaa tcgacatgtt gtacgtgttc ttgtccatgt cgacgaattc cggaattccg
      361 cccgcctgtg agatcgactc ggtcgtggca atgaaggtgt tcggcaccgt caccaccaca
      421 tcgcccggca caatgccgca tgccgtgtac gcgaagcgca gcgcgtcggt cccgctgttc
      481 acggcaaccg agtgggttgt gccgcagaac gcggcaaagg ctttttcgaa atcctcgacc
      541 atcggcccgc ccacaaaacc ggcggtgaaa agcgcacggc ggaaaactgc ggtgagttct
      601 tcggccagtt cgacgtgcgg ggttaccaga tcgagaaagg ggatgtcatt agacgcgtgc
      661 atggtggggg tcctctttgc tttcttccat ggaacgaagg atccgggccg ggttcccggc
      721 cacgatcgtg ttgggtggaa catctttggt gacaacgctc ccagcgccca cgatcgcgtt
      781 ttcacctaca gtgacgttgg acagcacggt ggcgcccgat ccgatcgatg cgccccggcg
      841 aataaccgtt ctctcgacct tccagtcagc ttccgtctgc aactgcccat cggccgccgt
      901 cgcccgcgga tagctgtcgt tgatgaaggt cacgccgtga ccgatgaaca cgttgtcctc
      961 gatcacgacc ccttcacaca cgaaggtgtg gctggagatc ttgcagcgct ttcccaccgt
     1021 ggcgttcttc tgaatttcca cgaaggcgcc gatcttggtg tcgtccccga cctcacagcc
     1081 gtagaggttg atgaacttcg acaaccgcac gttggtgccg agcttcacat catcggcaat
     1141 acagttaaaa gtgctcatag ataaacgaga gatcctcgct tgctgatgga ttcgctggct
     1201 gcttccagca tcttcaccac gcgcagcccc gcgcacccgt cgttgaacgc ctctttgccc
     1261 gtcgagatgc agtccacaaa gtacgacagc tcctggcgaa gcgcctcaac ctgctccagc
     1321 tgcggagacc acatatctcc cgaccggtag ttgaccagca acttgtacag gccttcgctg
     1381 ttgttgatgt tcacgccctt gtcgtagacc ttcaccttct cgtcgttctc gaggtcgttc
     1441 cacaccagca tgcgcttctc gccgccgatc agcgtggtgc gcaccttgac cggcgacagc
     1501 cagttcacgt tgatgtgcgc gatcacgttc tcgggaaaat acagcgtcat gaacgccacg
     1561 tcttcgtggg aattcagatg gcactgtccg gtggcgacga ccgcttccgg gctgccctcg
     1621 atcacgtggt ccatgatcga caaatcgtgc ggagccagat cccacagcac gttgatgtca
     1681 tgctggaaaa ggcccagatt gacgcgggtg gaatcgtagt agtagagctt gcccagcgcg
     1741 ccttcgcgca gcagctgcga gatcttccgc accgcccccg taaacaggaa ggtgtgatcc
     1801 accatgatcc gcagattctt ctgcagggca atgtcgatca gcttctcgcc ctgcgccacg
     1861 ttgctggtga acggcttctc cacgaacacg tgcttgccgt tctccagcgc cgccttggcc
     1921 agctcgtaat gcgtccagac cggtgtaatg accgcaatcg catcgatgtt cggagaagta
     1981 atgacctcca tcgcatcgga agtgatgtgg atgccggggt aggccttgtg agcccgtttc
     2041 tgagcggcgg gactgagttc cgcaattgcc gcaacctgag agtcttcgag ggaggcgaga
     2101 tttctgacga cgttcggccc ccaataaccg tagccgatta caccaaagtt cacgggagtg
     2161 tcaccacact ttccaggact gctgacaaac gaagagcgca ccacctcccg gtggagcacc
     2221 aaaggacaaa agaaacgaga aaggaattcg ggagcccgca ggagcgggcg aacctttagt
     2281 aggctccctt aatgaccgcg gccggcgtgc gcagaaggat cttgcagtcc agccacggcg
     2341 accatgagat cgcgtaacgc agatcgagcc gcaccatgtc gtcgaaacgg acccggctgc
     2401 gtccggtcac ctgccacagg ccggtgatcc ccggctttac ctgcagcacg cgccggcgat
     2461 gccaggtctg gtaggccgcc agctcgtaag gaagcggcgg gcgaggaccc accagcgaca
     2521 tgtcgccccg cagaacgttg aagaactgag gcagctcgtc caggctcgtc ttgcgcagga
     2581 acttgccgat cggcgtgatc cgcttgtcgc ccgccagctt gaatacctgc tggccattgg
     2641 ccgtctgcac accctgcgcc acaccggcaa tcagctgctt cacatattcc cggtgctcgc
     2701 tcgtatcgtt gttcaccttc atggagcgga acttcaggaa cgtgaatcgg cggccgtatt
     2761 gcccgatgcg atcctgccgg aacagcacag gacctttcga cgtcagcttc accgccagcg
     2821 cgatgatcgc gaacagcggc aggcaggcaa tcagcatcag gcttgcgccc gccacatcca
     2881 tggcccgctt cacgcgcagc agcacacgct ggttgttgtt gggaatgaac agatccggat
     2941 acagaatcgg atcgctcgga cgccccgagc tgtcactctc ccagtcatcg gggaagaagt
     3001 ggaacgagaa tgtgatctga ttgaagcgct ccagcgtcac ttcctgctgc agagcggtgc
     3061 tcactcggcc gaggatcgtg ctgagtacgg agttcttgtc actgatcacc agcccggtaa
     3121 agattacccc caccgtgctg cggttcttgt accagcccac cacgtcggtc tcgcgcgtgg
     3181 aagactgcat cgccgcgacg atgccggtca gcgtcttgtc cgaaccttcg ggggtgtccg
     3241 gaccggtggc ctccagcagc atgagcagga aaggttctct cgaccgttcc gtgcgctttc
     3301 gttcaatcgc gatccgccgc cggaacgctt cctcggctag cacgtcgcgg tctacgctgg
     3361 cggtcgtatc gaaaatgggg tctacgatgt ctgtgatttt attcatcctt ggcagagtca
     3421 caactcgctc ctctctcgag catcgatcaa tgttcgaaca tcaggtgcga aaaatagata
     3481 acggtccttt agaacttact cgcactggct ttacttcggc tcatgttttc ctgactgcat
     3541 cgatctccgg ttcccgagtc aaccggccct gctggcgcga acgaagtacc cgtcccgccc
     3601 tgaaattcat gcaatttcta actcgaccct ttgccgggcc ttgagttgta gtagtaatcc
     3661 gcgtgcggcg tgttattgga ggaattcaga atggcgccga tcagcttttg cgactccagc
     3721 gcgtccaccg ctctcagcat ctcgcgcttt tcgctgacgc cccgccgcgt gaccagcaga
     3781 actccgtcac acagccgcga ccacacgctg gtatcggcca gaggcaatac cggaggagaa
     3841 tcgatcacga tccagtcgaa aagctccccc agctgatcca gcagacgcga cagcttcgcc
     3901 gactgcagca gctccagcga attggacggc gcgctccccg cggtcatgaa ccacagcccc
     3961 gggccctcaa gcagataaat gctgtccttc agctcgcgat cttcccgcag gaactccgag
     4021 agccccggcc gcggcgccag cccgaacatc ggcgccatcg acggccgccg gatatcccct
     4081 tccagcagca gggttctctg cggcgcgcgc agcgccagcg agcacgccag gttcgccgag
     4141 cacatgctct tgccttcctg cggaatcgaa ctcgtgacca gaaccttctt gagcggccgg
     4201 tcgcgccgca ggttgcgcaa acgcaccgcc agcagacgaa acgcttccgc cgccgcgctg
     4261 tcccggtccg agatgctcac cagccgcccc tgcgccggaa cggaaacgcg cagcatctgg
     4321 aactggtcga agatcgccgg atgctccccg gcaagtgcag gctccgccgc cgcggctgcg
     4381 gaactgcggc tgccatgggc tgccccagac gcgccgttgg acgaacgccc ccgcccattg
     4441 cgcgccacgg ttttctccgg cttctccggt ttttccggcg ccgcaacctc atattcaggt
     4501 gtggcgtttt cggcaaaatc ggacaacgcg cgcttttccg cgtggcgcag cagttcagtc
     4561 gcatccgctg cggcaggtcc tttatctccc gaccgttcgg attctgaacg ctgcagggcg
     4621 tcaaatattc ggctcatact cactcgtttc gagagacaca ggcaaattca actcggcgct
     4681 gcgcgctgtc ggtttgcgca acgctgaata caagtcgcga agaactttcg cagcttgctg
     4741 gacgtccatt tcgttctggg ttccgcttcc tcccggctcg ctgaattctg agtcgagacg
     4801 gaactccctg gccacgtcgt cgacgatatc cgccgtgatg gccggtacct gtcgcgcgta
     4861 ggcggtaatc agtgcgtttt cgcagatggt gttgatcagc cgcgggaaac cccgcgaatg
     4921 acgatagacc gccgcaaccg catcggccgg aaagattcgc ttcggatctc cctccgctcc
     4981 tgccacctgc aggcgatggg cgatgtagtc ccgcgtctct tcggcgtcga gcgccgtcag
     5041 ctgcgcgcgc agcgcgattc tctgcttgag ctgccgcagt cccaccgagt ccagcttctc
     5101 atccagttcc ggctgcccca ccagcacgat ctgcagcagt ttgtcgtccg tcgtttccag
     5161 gttcgacagc agcctgacct cttccagcag ctcctcggac aggtggtgcg cctcatcgat
     5221 gatcagaacc gtcgtcagcc ccttcgatcc gcgcgacacc agaaattgcc ccaggtccag
     5281 cagcagctcg ctcttgttct tccccgatgc cggaagcccg aaatccgcca gaatgtattg
     5341 cagaaactcc gtcggtgaca gccggctgtt gaacagatag gcgtaagcaa tatccttgct
     5401 ttccttcagc aggcgaagca gacaccgcag cagcagcgtc ttgcccgttc ccacctcgcc
     5461 ggtcaccacc acaaatccct tgtggcgtcg gatcccgtaa tgcaaagcgg ccagagcttc
     5521 attgtgccgc cgggtcgaca caaaatacgt cgggtccggc gtcagatcga acgggttgcg
     5581 ggcgagctta aaaaaagcct tgtacatgaa ggacggcgcc tagccgtgca agtaactgat
     5641 tgcggatcct gcgagaatca ccatcgccac aaccgttgcc gtggcccatc cgatcaacat
     5701 ctttctccgg ttcacctgct cgtcgattgc attgctgacc gtcggaattt cacaaatcac
     5761 cggcaccggc agcaggtcgc tgatctccga ttcggagtgc agccggtcgt ccataaactc
     5821 gaaaccggcc accgaggccg cgccgaacac cagccccaca gccaaaccaa ccccgcagaa
     5881 caacagccga ttcggaaaat ccggcttggc cggcagactc ggcggatcga gcagctggaa
     5941 tcgctcgccc tgctgcatct gctccatgtt ggtcgccatc tgcgactcct gcatcttttt
     6001 caggatgtcg ttgtagttgg cctgcgactg attgtagccg cgctgcagat cggccagccc
     6061 ctgcacgctg ctcggcgtgg cgttgatgcg cgcctcgtac tcgttgattc tcaccttcag
     6121 agaatcgatc gctttctgac gattctggat tgccagattg tccgcctgca gttgtgcctg
     6181 cagctgcgcc accgtcagac tgtccgtcgt cttctcccct ttttcccgcg gagacgccat
     6241 cgcctgcttt cgctgctgct ccacctgcgc gatctgcgcc ttcagcttca ccacatccgg
     6301 atagctgtcg gtgtaccgcg aggtcagatc cgtcagctga tcgcgcagct tcgccagctg
     6361 gatatcgatc gcctgcacgc tgttgggatc gagcacctct ccggaacgga ctgcaaccac
     6421 cgggttggcg cgatactgct cgatctccgc ctcatgcaga gtgcgctgct ggtttgcgtt
     6481 gttcagcccg tcctgctcgt tgttcagttg cgactgcagg ccgcccagaa tcgccaggtt
     6541 gctctgcacc gagtccggca gcgctccgat atgcgtcgcc tggaagttct tcactttcgc
     6601 ttcctgatcg gccagcgtct gcgccgcctg atccagctgc gatttcaaaa accgggttgt
     6661 gtccgccgat tgctggtact ggttcttctg gttctcgttg atgaacagct cggccagctg
     6721 gccggtcacc gcctgcgcca cgcgtgcatt gtcggacgaa tagctgatct tgaatccggt
     6781 cacgctgttg tgcgtatcgc gcaccagatc gatatcgatc gctttggtca tggtcgcgac
     6841 tttgtcgtcg tcgctctgcg gcttgccccc gtcatcgtac agatgcatgc cgttgatgat
     6901 cgtcagcagc cgcgtgcggc tcaggatctc ctgctgcatg gtctgcagcc gcgcctgcaa
     6961 cgcgctctgg tccgccgccg catccgccac gttccgcggc agcgaaggct cctgcaccag
     7021 gatctgcgtg ctcgatttat accgcggggg aagaaaccag cttaccgccc ataccagaag
     7081 ccatcccgcc agcagcggaa tcagaaagta catatggcgc cgccgcccga tgtccagata
     7141 gcgtcccagg tcgaacttac tggctttttc ttcttcaaat acttctgaca tatttatctt
     7201 cccaaaggcc gggtgaagtg ataactcacc gcgcagaatt cccggttgct gatcggaatc
     7261 ggggacagcg aaacgaccgc tttgtaaacc tgatccatcc agttgtaacc gacatcgatc
     7321 ttcagattct tcgtcagcat atggctggcc gaaaccccgg ccgccagcgt gtgcccgccc
     7381 ggcacaacga aagcaaactg cggcgtcgcg ctcttgttct gcgtgtatcc ccccgagaag
     7441 ctcacgtccc acgtgggccg cagctgccgc cggaactgga tctccgcgcc gtcttcctcg
     7501 tacgctccgg gaagaccgcc gccccccgtc accgcccgcg ttatgctggc cgacgcgctc
     7561 gaatgatgcg cctgccatcc caacgacacc gtgccggccg gggtccattg cgagagcagc
     7621 gcctccgggt actgaatgat gctgtagtgc tccggccccg ccgtaatcga gatcgaaaac
     7681 gtgctctgcg gcagggcgcg cagataaatc gtgtagaacc cgaaaatgtt gtcctgcttc
     7741 atcacgttat cgacgccgtt aacgtagctc acaaccttcc cgtgctgcgc gttggcgccc
     7801 agatactgcc gccggctcag ccgctgcgtc acgaacgccc cgccctgata ggaggtcgcg
     7861 ttgtaaagtc ccgtcacctg gcggttatcc ggataatcca gattgctgaa gttggccgac
     7921 aagccgatca tcccgttggt gcccgtctgg ttcgtcagct gcgctgcggc cgtgttcatc
     7981 agccggtccg caaacggcgg aagcacgccg ctggtctgcg tcggcactcc tcccgaaatt
     8041 ccctggctgg tcccgaacgg ctgattgaac aaactcgaac tgcggtcgaa attgtccgac
     8101 agatccagaa ccgtatgcgt gcccatctga tactgtagat ccgccgtcag attatggtcc
     8161 atcgcgttga agaccgtgct gggctgatag aacgtgaatc ccgggctgta gttcagcacc
     8221 tcgcgaagcc gcgtcgaact ccggttcacg ctgatcgtcg gccacagcga atagatcatg
     8281 tcccccttcg atacgcccgt gtagccgcct tctacgttat cgtcgtaagc gacattcgcc
     8341 gtggcgccaa gagtcatgaa attcctgcgc tcctccgacc ccgtcagaat cggatacgtc
     8401 tgcccgctca ccggcggtgg cgtcagcatc ccgtcgttcg ccagcgggtt gtctggctgc
     8461 tcgtcggtcc cggtgctggt cccggggtta tcggtgcccg ttcccgtggc ctgtgaccat
     8521 gcggggatcg ctgcacacag cgccaaaagc agaacaatgc gcggggtcat agtcagtctc
     8581 atggaacgac gactgtgtct cccgactgca gaagaatatt ctgtttcggg ttcttgccct
     8641 tgatgaaggc gctgtagttg aacgagagcc gcgcctcagt cccgtccggc ttctggtgca
     8701 gcacgtaaat cgatttcttc ttcgcaaaat cgcggaaacc gcccgccgtc gcgatcgcat
     8761 ccacaatcgt cgtccgcgcc gccagcgaat acgaccccgg ccgcgccact tcacccatga
     8821 cattgaactt caggctgttg atcttcagca ccatcaccgt cacggccggc tcggtgatga
     8881 agctccgcag cttcgtcgtg atctcgtctt ccagctgcac cggagtcttg cctgccgcct
     8941 ggatctctcc taccagcggc atggagatct ttccgtccga ccgcaccgtc agctgcttgc
     9001 tgagctccgt ctctttccag acgtcgatct caatcatgtc gtcgtcgccg atcacatagg
     9061 tgctgttgtg ggcgtgattg agcggtgagg gagtcacctg atcagaagag gcttccggag
     9121 cagaaggcga atcgggagtc gaattgctgg aagccaccgt ctgcgcgggc gttaaagctg
     9181 caaatgcaaa aaacagaacg cttcctgtaa ccagggcata aacagtattc agtttcataa
     9241 cctgcaacaa cctcttgggg gaaccatcgg gcgaacagcc tgagcgcccc tcctgctgta
     9301 ctgggtgatt gtgagccggt caggctcttg gtatcagatt tctgaggagc gttgtcctat
     9361 cttgcctctt aattcggacc agcgaaaggg aaattcttct ggaaatctcc aattcctgtt
     9421 tccacgattc cacccggtga acttttcggg tttgcggcgc ccaccggctc cacgtccgtc
     9481 gccgcaacct ccacccccac cgactgggcc agcatctcaa ccgagagcac cagacggtag
     9541 aggttcttct tacgtaccag gattccttca accccttcca gagacccgcg cgtcacccgg
     9601 acccgctctc caatcttcag gaacgggtgc ggttccactc gatacgagct ctggaccgcc
     9661 cgtcgaatcg catcgatttc cggctccggt accacctcga tcttctcgcc gcgcgacagg
     9721 atcatatgga tcccaggcgt gctcaccacc tgcagccgcc ggctcaaaat tccgcgaacg
     9781 aaaacgtacc ccggaaaaag cggcaaagag atgagcttct gccggtcctt ccaccgccgc
     9841 aaggtctcgt gcaatggcag gaagacatcg aaacccttcc ccgccagcac ctcggcaacg
     9901 gtcttttcgt gctggtgctg cgtgtaaacc gcccaccagg cgggtgcgtc ggcgagatcc
     9961 tcggtgttgg ctgcgcccgg catcttgttc gctcca
//
LOCUS       DASZYN010000480         2199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_84667, whole genome shotgun sequence.
ACCESSION   DASZYN010000480 DASZYN010000000
VERSION     DASZYN010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84667"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..542)
                     /locus_tag="VHE33_12405"
     CDS             complement(132..542)
                     /locus_tag="VHE33_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98301.1"
                     /translation="MASLTMILLAALLCNYHRGKCLIEIGTEGIFVSGHHPIHGTQIL
                     YFSDPVLAKRAAIPPPTEFAAIEDGRVNPQRAADRTANVISQPEVVTEPASTMERAFV
                     DALSGRAAATAGRQTGQTPKHGFLEQLSFEDRED"
     gene            526..1943
                     /locus_tag="VHE33_12410"
                     /pseudo
     CDS             526..1943
                     /locus_tag="VHE33_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611236.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1940..>2199)
                     /locus_tag="VHE33_12415"
     CDS             complement(1940..>2199)
                     /locus_tag="VHE33_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98302.1"
                     /translation="DEALRVRVLETLAARASSLVKDLTPEDAVAIAGVALRALQLTFE
                     TEGLEFAAFLHGTDKAEEDYSVGDSIDTVRVNKNETHAVKV"
BASE COUNT          416 a          685 c          642 g          456 t
ORIGIN      
        1 tcgttccggt ccttatagcg atagccctcc gcgagacgag cgttcgcgac cggagtcccg
       61 gagggcaaat accggacttc aggcttcgcg gcgagatagc ccgcaagctc gattctgttc
      121 ttctgcattg tctaatcctc cctgtcctca aagctgagtt gttcaaggaa tccgtgcttt
      181 ggtgtctggc cggtttgcct gccggctgtc gccgcggccc gaccggaaag agcgtccacg
      241 aaggcacgct ccatagtcga tgccggctcc gtgacgacct ccggctgact gattacgttc
      301 gccgttctat ccgctgctct ttgtggattc acgcggccgt cctcaatcgc tgcgaactct
      361 gtcggaggcg ggatcgccgc ccgtttcgcc aagactggat cggagaaata gaggatttga
      421 gttccgtgga tggggtgatg accgctgacg aagatcccct ccgtgccaat ctcgatgaga
      481 cacttaccgc ggtgataatt acagagcagg gcggcaagga gaatcatcgt gagcgatgcc
      541 aagaatgcag ttcctttcgc aaccagttcg ggggatgtgg aagtgccggc caaagcacaa
      601 cggcgcatct ttaccgcgga agagaagaag cggattttag aagaaaccga ccgcgcgctc
      661 gcaaatggcg gtggtgtggg cgccattctt cggagggaag ggatctactc ctcgaccttg
      721 cacggctggc gcaaggagcg cgatcgcgcg gttcacaaag ccttttctca gaagaggggt
      781 ccgcagccgc aacggaatcc gttggccgcc gagaacgaga agttgcgccg gcagaatcag
      841 cgtttgcagg aagagctgga gaaagcccac atcattattg atgttcaaaa aaaagtcgcg
      901 aagctgttgg gtcacccgat cccggaaacg gagaagtcgt gatggccgcg gtagatcaac
      961 tcagcccgct ggtcggtgtg aagccggcct gccaggcgtt ggcggttccg cgagccacgt
     1021 ggtaccggcg gcgcagcggc cgtctttttc cccggccagc ggccgggccg cggtcctcgg
     1081 ctcgggcttt gaccgaagct gaacgccagg cggtattggc ccgtctgcac gaggatcgtt
     1141 ttcaggactc ttcgcccgcc caggtctacg cggcgctgct cgatgagggc cggtttcatt
     1201 gctcgatccg caccatgtat cgcttgctgg aagcacgtgg ggaaagccgc gagcgccgcg
     1261 atcagctgac tcatccgccg tatcagaagc cggaactgct ggccactcaa gccaatcagt
     1321 tgtggagttg ggacatcacc aaactgcgcg gtccggcgaa atggacctgc tatcacctgt
     1381 acgtcatctt ggatgtcttc agccgtttcg tggtgggctg gatgatcgct tatcgcgaaa
     1441 gcgcggaact ggctaagcgt ttgatcgatc attcgtgccg caaacaactc atcgcgcccg
     1501 gccagctgac catccacgcc gatcgcggct catccatgaa atccaaaccg gtggctctgc
     1561 tgctgtccga cctgggcgtc accaagacgc attcgcggcc gcatgtgtcc gatgacaacc
     1621 cctattccga aagccagttc cgcaccctca aataccgccc tggtttcccg gatcgtttcg
     1681 gctccatcga agacgctcgt gggttttgcc aatccttctt ccgctggtac aactacgagc
     1741 accaccactc cggcctgggc ttgatgaccc ccgccgtggt tcacttgggc aaggccgccg
     1801 acctcctggt ccatcgccaa tccgtgttgg actccgcttt tcaggctcac ccggaacggt
     1861 tcgtccgcca gcctcccaaa cccccttctc tcccttctgc ggtctggatc aataggcctc
     1921 ccgacaagga gaaaactcct taaactttta ccgcgtgtgt ctcattttta ttgacacgca
     1981 ccgtgtcgat gctgtccccg acgctgtaat cctcttcggc tttgtccgtg ccgtgcagga
     2041 acgcggcgaa ctccagtccc tccgtctcga aggtgagctg cagcgcacgc agggcgaccc
     2101 cggcgatggc gacggcatcc tccggcgtga ggtccttcac gagactcgat gcccgcgccg
     2161 cgagcgtctc gagcacgcgc acccggaggg cttcatctt
//
LOCUS       DASZYN010000481         3298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_276108, whole genome shotgun sequence.
ACCESSION   DASZYN010000481 DASZYN010000000
VERSION     DASZYN010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276108"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VHE33_12420"
     CDS             <1..432
                     /locus_tag="VHE33_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98303.1"
                     /translation="ALRTAVILDTCFSGGAVTNASRMMGKGIANTAPGAETLDRMAQG
                     TGRIVIAAARSDEESLESDQLHHGYFTYYLLQAIKANKGLDPMSAVYATVAKEVADRV
                     AADGKANGETVGQHPVMSRSSDNSDFALGITAADAVSKGGR"
     gene            942..1457
                     /locus_tag="VHE33_12425"
     CDS             942..1457
                     /locus_tag="VHE33_12425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVT98304.1"
                     /translation="MDQSPETFRAVVERHQARVYSIALRILGDSGVAEEVAQDVFLEL
                     YRAMPRLDGESHVTAWLRRVACHRATDALRRRAARGDGAAEEYQDGMALRPVVREFLP
                     LMNRVEQLLLTLPPPQREVMVLRYQEDLEPEDIALELKMPLATVRSYLQRAVKMLRAK
                     AERTLKEYSRG"
     gene            1450..1827
                     /locus_tag="VHE33_12430"
     CDS             1450..1827
                     /locus_tag="VHE33_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98305.1"
                     /translation="MVEPRDDFEQELRQQLQARPAPEGFADRVMARVPKRPARSGWVG
                     FRTPVWQWAVAALLVLGMVVGGLERERQQRIEGERARDQVLLALRIAGSTLRDVQDKV
                     NAGASQQKLSQKGAGRAVLDRDQ"
     gene            1847..2473
                     /locus_tag="VHE33_12435"
     CDS             1847..2473
                     /locus_tag="VHE33_12435"
                     /inference="COORDINATES: protein motif:HMM:NF025426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HVT98306.1"
                     /translation="MRWASIRNVASVAALIAAMGFSALAQQPPFPVTLQKQLADRAAN
                     YTEVSLDRKMLAFATHFLNDKDNADAKRIVEKLNGVYVRSYEFDQPGQYTAADLDAIR
                     RQFDTSQWTAMVKTRSKNGSDDADIYLKMVNDEVQGMFVLDAESKELTFVYISGPIHP
                     EDLRDLSGNFGIPAGTYEFSVKEKEKGQKKAESKAQKSETKTQKEGQP"
     gene            2470..2988
                     /locus_tag="VHE33_12440"
     CDS             2470..2988
                     /locus_tag="VHE33_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98307.1"
                     /translation="MKMRSFVIPAVLVTAIFAIVLPLTVRAAGHHDFDAVVSAVEHQY
                     DARPQRIPMMGFVSFCGWVATGGGVKGLKIAEFDHFAGPADPAELDKLVDSSLGAEWQ
                     RFVANRERNGELNLIYAQPDGHAMRMLIVDYEHGELDVVRVEVNADRLQHWMKDPGGS
                     AKRHDYGTERPD"
     gene            complement(3112..>3298)
                     /locus_tag="VHE33_12445"
     CDS             complement(3112..>3298)
                     /locus_tag="VHE33_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98308.1"
                     /translation="NVAEMFGFGDKLGSLDAGKMANVVVANGDPLDVRTDVKQVYIQG
                     VAVPMVSRQTLLRDEYQ"
BASE COUNT          738 a          978 c         1024 g          558 t
ORIGIN      
        1 gcgctgcgca ccgccgtgat cctggacacc tgcttcagcg gtggcgccgt gaccaacgcc
       61 tccaggatga tgggcaaggg catcgccaat accgctccgg gagcggagac gctcgatcga
      121 atggcgcagg gcacaggccg catcgtgatt gcggcggcgc gatcggatga agaatcgctc
      181 gaaagcgatc agctgcacca cggctacttc acgtactacc tgttgcaggc catcaaggcg
      241 aataagggcc tcgatcctat gagcgcggta tatgccacgg tcgcgaaaga agtcgcggac
      301 cgcgtggcgg cggatggcaa ggccaacggc gaaacggtcg gccagcatcc ggtcatgagc
      361 cgcagctccg acaactccga ctttgccctc gggatcaccg ctgccgacgc agtttcaaag
      421 ggcggccgct agtgtggtgt cccgcaagtt cttcagctgt gccataaagg cagcagaatg
      481 actccggctt gaccctctga gggaagctct tcgcctgcgt ccgcactctc ccccggcgat
      541 acagctaagc cgaacgaaaa tcagcggtgc agaacaaaaa ccaatcgtgc agaacgaaaa
      601 aattctgcag aaggaaagaa atcgaccaga cacgaaagtc aaatttcgtg gggcttccag
      661 ccccgcgaag acggtagaag ccccgggctt cagcccgggg aacaacccgc aacaaagatt
      721 gggctttagc cccggggttt tgaaaggtcg tgccgcaaag gcgcaaaaaa ggggacccgc
      781 tcctcttcag cacaaagaga tttccgaaac actacatctg tgcaggttca accccgccga
      841 acgctgatcg ctcatcacaa cccaaaaata tttgcgaatt ttgcatcatc cggcatgccc
      901 ttttgcgtat atgcgggtac agcggtttga gggcacgccc gttggaccag tcgccggaga
      961 catttcgagc cgtcgtcgag cgccatcagg cgcgggtcta ctcgatcgcg ctgcggattt
     1021 taggagacag cggtgtggcc gaagaagtgg cgcaggatgt gttcctggaa ctctatcgcg
     1081 ccatgccgcg ccttgacggc gaatcgcacg tgaccgcgtg gctgcgcaga gtcgcctgtc
     1141 atcgcgccac cgatgcactg cggcgtcgag cagcgagagg tgacggtgca gcggaggagt
     1201 atcaggatgg aatggccttg cggccggtgg tgcgggaatt cctgcccctg atgaatcggg
     1261 tggagcagct gctgctgaca ctgcctccac cgcagcggga ggtcatggtg ctgcgatacc
     1321 aggaggatct tgaaccggaa gacatcgcgc tcgagctgaa gatgccgctg gcgacggtca
     1381 ggagttatct gcagcgcgcg gtgaaaatgt tgcgggccaa agcggaacgc accctgaagg
     1441 agtacagccg tggttgaacc gagagacgat ttcgagcagg agttgcggca gcagttgcag
     1501 gctcggcccg cgccggaggg ctttgcggat cgcgtgatgg cgcgcgtgcc gaaacggcca
     1561 gcgcgcagcg gctgggtcgg attccgcacg ccggtctggc aatgggccgt cgccgcgctg
     1621 ctggtgctgg gaatggtggt cggtggcctc gagcgcgagc ggcagcagcg catcgaaggc
     1681 gaacgggcgc gggatcaggt attgctggca ttgcgtatcg cggggtcgac gttgcgcgat
     1741 gtgcaggaca aagtcaacgc cggtgcctca cagcagaaac tcagccaaaa gggcgccgga
     1801 cgcgcagtcc tcgataggga tcagtaataa ggaagaggga tgaaccatga gatgggcatc
     1861 gatcagaaat gttgcttcgg tcgcggcgct gatcgccgca atgggatttt cggccctggc
     1921 acaacagccg ccgtttccgg tgactctgca gaagcagctt gccgaccgcg cagccaacta
     1981 caccgaggtc tcgctcgaca ggaagatgct ggccttcgcc actcacttcc tgaacgacaa
     2041 agacaatgcc gacgcaaagc gcatcgtcga gaagctcaac ggcgtctacg tgcgcagcta
     2101 cgagttcgat cagcccgggc agtacaccgc ggccgacctc gatgccatcc gtcgccagtt
     2161 cgacacatcg cagtggacgg ccatggtgaa gacgcgatcc aaaaatggat ccgacgacgc
     2221 cgacatctac ctgaagatgg tgaacgatga ggtgcagggc atgttcgtcc tggatgccga
     2281 gtcgaaggag ctgaccttcg tgtacatctc cgggccgatc caccccgagg atctcagaga
     2341 cctcagcggc aacttcggta tccccgccgg aacttatgaa ttcagcgtga aggaaaaaga
     2401 gaaggggcag aaaaaggcag agagcaaggc ccaaaaatcc gagaccaaaa cgcagaagga
     2461 ggggcagcca tgaagatgag gagtttcgtg attcctgctg ttcttgtgac cgcgatcttc
     2521 gcgatcgtcc tgcccctgac cgtgcgggca gcgggccacc atgacttcga tgccgtggta
     2581 tcggccgtcg agcatcagta cgatgcgcgc ccccagcgca tccccatgat gggcttcgtg
     2641 agcttctgcg gctgggtcgc gacgggcggc ggagtcaaag gcctgaagat cgccgagttc
     2701 gatcattttg caggccccgc cgaccctgcc gaactcgaca agctggtgga cagcagcctc
     2761 ggcgcagaat ggcagcgatt cgtcgccaat cgggagcgca acggcgaact aaacctgatc
     2821 tatgcgcagc ctgacggcca cgcgatgcgc atgctgatcg tggactacga gcatggggag
     2881 ctggacgtcg tgcgcgtcga agtcaacgcc gatcggctgc agcactggat gaaggatcca
     2941 ggcggcagcg cgaaacgcca cgactacgga accgaaagac cagactaaga gcagcagcca
     3001 gtagccaata gccagcaggc aacagagcgc ccgacgatgc atcgaaaccc tactgcctac
     3061 tgcctactgc ctactgccta ctgcctactg cctactgcct actgcctgtt cttactgata
     3121 ctcatccctc agcagcgtct gccgactcac catcggtacc gcgacgccct ggatgtacac
     3181 ctgcttcaca tcggtgcgga catcgagcgg atcgccattc gcaaccacga cattggccat
     3241 cttgccggcg tcgagcgaac caagcttgtc gccaaacccg aacatctccg caacattc
//
LOCUS       DASZYN010000482         1645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_276133, whole genome shotgun sequence.
ACCESSION   DASZYN010000482 DASZYN010000000
VERSION     DASZYN010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276133"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            376..1542
                     /locus_tag="VHE33_12450"
     CDS             376..1542
                     /locus_tag="VHE33_12450"
                     /inference="COORDINATES: protein motif:HMM:NF033577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HVT98309.1"
                     /translation="MDERLRFVARLLDGEKMAPLCREFDISRKTGYKIFERYKDCGVI
                     GLTDRSRRPYRHANQLPFQIEKQIIELKRERPSWGAPKIREKLRRLYPEVQCPAISTV
                     HAVLDRNDLVKRRRRRRYKAEGTPLSRPLEPNDLWCADYKGEFMLADKRYCYPLTVTD
                     SASRYLLACEGLATTNGMFARHVFERIFREFGLPRAIRTDNGVPFASAHALFGLSRLS
                     VWWLRLGIEIERIKPGCPQQNGRHERMHLTLKQEATKPASANFLHQQARFDRFVEIYN
                     DERPHQALGMKYPGECYSRSARSYRGQLPELDYPFHDKTVTVTTCGRICYQHQKVNLS
                     TVFAGQKVGIRQIDEQIWLVSFMHYDIGFFDDETCRLEPGPNPFGAKVLPMSPE"
BASE COUNT          349 a          515 c          477 g          304 t
ORIGIN      
        1 tcatgccacg gtcccaaaac ttaagagagt gtaaacaaat gtcaacaccc ttgagttcct
       61 ttgctgcttc gctgctttcg aattccttaa cggtgttaag aattttgcac agtgggggcg
      121 aagaacggcg gcagggtaat ccgcgttccg catccgaagt taccttgaaa gttacctcgg
      181 ttaccctgcg attgcatgga acgtcatgag acggcatggg aaaaggaaag tcgtgcaatc
      241 tcaatgacta gcgaccggtt ttgggacgct atgggacggg atgagagtga tgggtgacag
      301 ctgataccgg agacatgggt tacactctag gcctttgcgc gggagggcaa agtgccgtgg
      361 aaggagtgtg gcaaagtgga cgaacgtctc cgcttcgtgg ctcgcctgct cgacggcgag
      421 aagatggcgc cgctgtgccg ggagttcgat atctcccgca agaccggcta caagatcttc
      481 gagcgctaca aggactgcgg tgtcatcggc ctcaccgatc gctcgcggcg accctatcgt
      541 cacgccaacc agctgccgtt ccagatcgag aagcagatca tcgagctcaa gcgtgaacgg
      601 ccgagctggg gcgcgcccaa gatccgcgag aagctcaggc gcctctaccc cgaggtgcaa
      661 tgcccggcga tcagcaccgt ccatgccgtg ctcgaccgca atgacctggt gaagcggcgt
      721 cgccgaaggc gatacaaggc cgaagggacg ccgctctcgc ggccgctcga gcccaacgat
      781 ctgtggtgcg ccgactacaa gggcgagttc atgctcgccg acaagcgcta ctgctatccg
      841 ctgaccgtca ccgactccgc cagccgctac ctgctcgcct gcgaaggcct cgcgacgacc
      901 aatggcatgt tcgcccgcca cgtcttcgag cgcatcttcc gcgagttcgg cctgccgcgg
      961 gcgatccgca ccgataacgg cgtcccgttc gccagcgccc atgcgctgtt cggactcagc
     1021 cgactgtcgg tctggtggct gcgcctcggc atcgagatcg aacgcatcaa gcccggctgc
     1081 ccacagcaga acggtcgcca cgagcgcatg cacctgacct tgaagcagga agcgaccaag
     1141 cccgcatcgg ccaacttcct ccaccagcag gcgcggttcg atcgcttcgt cgagatctac
     1201 aacgacgagc ggccgcacca ggcgctcggc atgaagtacc cgggcgagtg ctacagtcgc
     1261 tcagcgcgaa gctatcgcgg ccagctgccc gagctcgact acccgttcca cgacaagacg
     1321 gtcaccgtca ccacctgtgg tcgcatctgc taccagcacc agaaggtgaa cctcagcacc
     1381 gtcttcgccg gccagaaggt cggcatcagg cagatcgacg agcagatctg gctcgtctcc
     1441 ttcatgcatt acgatatcgg cttctttgat gacgagacct gccggctcga gccgggacct
     1501 aacccgttcg gagcaaaagt gttacctatg tctccggaat aatctgtaac ccatgtctcc
     1561 ggtacagacc ataatcagct ggatgccccg tgtggattcg aaccacaatt gacggagtca
     1621 gagtccgtag tcttaccatt agacg
//
LOCUS       DASZYN010000483         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_855009, whole genome shotgun sequence.
ACCESSION   DASZYN010000483 DASZYN010000000
VERSION     DASZYN010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855009"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VHE33_12455"
     rRNA            complement(<1..858)
                     /locus_tag="VHE33_12455"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(1332..>2266)
                     /gene="tyrS"
                     /locus_tag="VHE33_12460"
     CDS             complement(1332..>2266)
                     /gene="tyrS"
                     /locus_tag="VHE33_12460"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HVT98310.1"
                     /translation="DGPTDAAMVNNLDWTKDLSAIDFLRDVGKHFTVNQMLGKESVSA
                     RLEGGISFTEFSYMLLQAHDFLALNQRHGCLLQVGGSDQWGNITAGLDLLRKVGGVSG
                     HALTAPLIMNASGKKFGKSETGESVWLDPAMTSPYAFYQWWLNTDDRDVAKHLRTFTF
                     RSREETEALVQAAAERPQTREAQRALAEDMTRLVHGPEQLARAQAASQALFGRSALEE
                     LDEATLAAALAETPLVAVGPGPLPPVVELLADAGLVESRSAARRVVQEGGAYLNNRKV
                     EDITAVPGEGDLLHGRFLVLRRGRQKVAGVERRA"
BASE COUNT          344 a          786 c          707 g          429 t
ORIGIN      
        1 ccaggcgggg aacttaatgc gttagctgcg gcacggacaa cgtggaatgt cacccacacc
       61 tagttcccaa cgtttacggc gtggactacc agggtatcta atcctgttcg ctccccacgc
      121 tttcgctcct cagcgtcagt aatggcccag agatccgcct tcgccaccgg tgttcctcct
      181 gatatctgcg catttcaccg ctacaccagg aattccgatc tcccctacca cactctagcc
      241 tgcccgtatc gaatgcagac ccggagttaa gccccgggct ttcacatccg acgcgacaag
      301 ccgcctacga gctctttacg cccaataatt ccggacaacg ctcgcgccct acgtattacc
      361 gcggctgctg gcacgtagtt agccggcgct tcttctgcag gtaccgtcct gattcgcccc
      421 tgctgaaagc ggtttacaac ccgaaggcct tcatccccca cgcggcgtcg ctgcgtcagg
      481 ctttcgccca ttgcgcaata gtccccactg ctgcctcccg taggagtctg ggccgtgtct
      541 cagtcccagt gtggccggtc gccctctcag gccggctacc cgtcgtcgcc ttggtaggcc
      601 gttaccccac caacaagctg ataggccgcg ggcccatcct cagccgatga atctttccac
      661 ctcaggagat gtctcccgag gtcgtatccg gtattagccc cggtttcccg gagttgtccc
      721 agagctgagg gcaggttgcc cacgtgttac tcacccgttc gccgctcgag tacccccgaa
      781 ggggcctttc cgctcgactt gcatgtgtta agcacgccgc cagcgttcgt cctgagccag
      841 gatcaaactc tccgtgaatg tctattaccc agtggacccg aaggtccatg agtgacacag
      901 gagccggcct ccctggcggg agaccggttg atctctggcg ctggcatgta gatccgtaga
      961 tcgtcatgtc aatcaccaaa ggaattccga cccggggggc ttgaatcccc gggacggggt
     1021 tttggcactg actttcggca cgctgttgag ttctcaagga gcagacgcgc actgtcccgg
     1081 gtctctcgac ccgtcgcagg gcaacttgcc gaccatagtc gcgggctcgg gcacggtcaa
     1141 ccgcaagctt ccttgcgggt ggccttggct ccggggctct cgctccctgg cgggcttgaa
     1201 aaccatacac caggtgaacg ggtgctgcgc gccggaacgc gggaagtcgt cccgtgcgcg
     1261 ctcctggatg cccggaaccc ctgtcgttgc agggtttcag gccctccgga gatccttcgc
     1321 gcaagccggt ctcaggcgcg tcgctcgacg ccggcgacct tctgccggcc cctgcgcagc
     1381 accaggaagc ggccgtggag caggtcgccc tcccccggca ccgccgtgat gtcctcgacc
     1441 ttgcggttgt tgaggtaggc gccgccctcc tgcaccacgc gccgggcggc cgagcggctc
     1501 tcgacgaggc ccgcgtcggc caggagctcc accaccggcg gcagcgggcc cgggcccacg
     1561 gcgacgaggg gtgtctcggc cagcgccgcc gcgagcgtcg cctcgtcgag ctcctccagc
     1621 gcggaccgcc cgaacagcgc ctggctggcc gcctgggccc gggcgagctg ctccggcccg
     1681 tggacgaggc gtgtcatgtc ctcagcgagc gcgcgctggg cttcccgggt ctgcggacgc
     1741 tcggccgcgg cctgcaccag ggcctcggtc tcctcgcggg agcggaaggt gaacgtccgg
     1801 aggtgcttgg cgacgtcgcg gtcgtcggtg ttgagccacc actggtagaa ggcgtagggc
     1861 gaggtcatgg cggggtcgag ccacacgctc tcccccgtct cgctcttgcc gaacttcttg
     1921 ccgctggcgt tcatgatcag cggcgcggtg agcgcgtggc cgctgacccc gccgaccttg
     1981 cggagcaggt ccagaccggc ggtgatgttg ccccactgat cgctgccgcc cacctggagc
     2041 aggcagccgt ggcgctggtt gagggccagg aagtcgtgcg cctggagcag catgtagctg
     2101 aactcggtga agctgatccc gccttcgagg cgggcgctca ccgactcctt gccgagcatc
     2161 tggttgaccg tgaagtgctt gccgacgtcg cgcaggaagt cgatggccga caggtccttg
     2221 gtccagtcga ggttgttgac catggccgcg tcggtcggcc cgtccc
//
LOCUS       DASZYN010000484         5974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_664377, whole genome shotgun sequence.
ACCESSION   DASZYN010000484 DASZYN010000000
VERSION     DASZYN010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664377"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1935
                     /locus_tag="VHE33_12465"
     CDS             103..1935
                     /locus_tag="VHE33_12465"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HVT98311.1"
                     /translation="MPDSPAQIVTGFSQAAVGAIPTAADTARPLPEAAVPERPGDAAM
                     RPGMSLMIEKIAPTRDERLFLMLSIFIGILSGLLVVAFRIAIEWIKILTLGSAPHAGQ
                     YRLLLVPAIAGLVVSALVIWFFPAARGSGVNQTKAALYVYEGYISFRTVIGKFLTSAL
                     AIGAGHSLGPEDPSLQIGAGVASIIARNLRLSRRRLRMFAPIGAAAGLAAAFNAPISA
                     ILFVIEEVIGSWSAAVLGSLVLSAISSVVVARWFWGPEPMFRIPSITLRDPRELMAYA
                     LLGVFGGLVAPVFSSILGYLRPRLRAVPRWNIFLQPAVAGLLVGAIGFFGFPQVMGTG
                     YDVIDQALHGQFVWQVLLALVFLKLMATTFSFSSGTPGGMFAPTLFIGAMLGGAVGAF
                     EKHFFPHLTGTVGAYSLVGMGVLFAGFLRVPLTSVFMVLEVSGNYSIVLPIILANTLA
                     FLISRSLQPVGIFEIFTQQDGLDLPSMEEQREESSLHIEDALQPVSVPVVAGSDTIQS
                     ASEAVSETHSMGCMVRLSDGSWYALSADELAGAGATLTPEAPVQRALKEDRAPLLFPD
                     MPLDSALHHFPRWSFLPVLNRASRGTLEGIITMNDVLKRYRSPE"
     gene            2008..3822
                     /locus_tag="VHE33_12470"
     CDS             2008..3822
                     /locus_tag="VHE33_12470"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT98312.1"
                     /translation="MLSLYQRLIFGCVLLIALVTAVSMLVRTSFVHLAALDAQVQVAQ
                     TAVASLASAQGAVAHEELIAARIATGTASLREFREQEGRTQALLRTAVDDVRAFDPSL
                     PVDRLVSDNERLAQHSPVDPGGLTRSLEALHVELRRDLEQLSARRDSTVTALHHQQDW
                     LRERLIAACGLSIAAATVISAAMLFLVITPLRRTARIARRIGQGDLHQRVEWRSKDDL
                     GTIATELNRLAIRLRDLRETESGRRQMEFQLSDAVVRSIFEPVIVTDGKGQVLKLNQS
                     AIELLGGDSADGEVDRMVLTSTPGGDKILSAIRAAVSMQRAMTGGEGEAALLPMRIGK
                     EQRSYRLRTTPMRDSEGKLLGAVTLLEDVTEIAEVDRFKSRFLAVASQKLREPIRRLR
                     LALFAITQGHSGELRPLQMDLATSAAHEAEQLDDLMLDLIEVAELETGRRQLRMEKIR
                     PTDALRDSAARCRDAVRGKNIDMEILAFDDVAYVQADRRALRTILDNLITNALRYTPE
                     GGHIRIGATEQQDRVQFFVHDNGRGIEAERLPTIFGRFTGDPNSGTSGLGLALVRRLV
                     ESQGGEVSVESRLGSGTTFRFTLPIAVAEPDRHPVEVG"
     gene            3819..4997
                     /locus_tag="VHE33_12475"
     CDS             3819..4997
                     /locus_tag="VHE33_12475"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVT98313.1"
                     /translation="MTTPPSIPPEDSTPEPLRPVTPVQTDHEPGRLRVYLGAAPGVGK
                     TYRMLEDAHLMRRQGVDIVIGLIEPHGRADTAALIQGLDIIPLQEIQYRSVTLREMNI
                     DAVLARHPAWCVVDELAHSNVPGSRNHKRYEDVLELLDAGINVMTAMNVQHLETLNDA
                     VARSASTQIRETVPDSFLRRADEVVNVDITIDELRDRLRQGKIYAPEKVEQALANFFR
                     KGNLNMLRELALRTTAEQVGTAAAEYRRNQGLEQAPIPEKVMVCLSPRAGMERLIRVG
                     ARVAGRLATNWYVVYVETPETDHRHGDPAASARLEDYERMARDLGARIVKLKDRKVAD
                     ALIEFARKENISHVVFGQSARTRWDILLRGSIINRFLNEMRDTTVQVVPVQKEVRGRA
                     "
     gene            5026..>5974
                     /locus_tag="VHE33_12480"
     CDS             5026..>5974
                     /locus_tag="VHE33_12480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HVT98314.1"
                     /translation="MNRDAMLARIRDRAEPFDLVIIGGGATGAGIAVDAASRGFSTLL
                     LEREDFGKGTSSRSTKLVHGGVRYLEQGNLSLVMGALRERGILRRNAPHLVHDVPFVV
                     PNYSWWETPFYGIGLKIYDLLAGQYGFGKSKLLSRDETLERLPTLRQDGLRGGVIYHD
                     GQFDDARLLIHLLATAADYDATVLNYAPAVEIRRGSDGFVCGVVAEDRESGERFSIDA
                     QVVINATGIFADETRRMADANAAPMMVPSQGIHLVLEGSFLRGNTAIMVPHTSDGRVL
                     FAIPWHGHTVVGTTDTSIDGPSYEPLPFEHEIEFVLETAS"
BASE COUNT         1060 a         1916 c         1833 g         1165 t
ORIGIN      
        1 gacaccgaaa cggccaccgc ggcggtgtgc ggcgcgcccg aaggagacga cgcgacggtg
       61 ctgcgcattg cccgaaatcc aaacacggtg gaacaggagt agatgcccga ctcgcctgca
      121 caaatcgtca caggtttttc ccaggccgcg gtcggggcaa tcccgacagc tgcggatacg
      181 gctcggcccc tgccggaggc ggcggttccc gagcggccgg gcgacgcagc gatgcgcccc
      241 ggcatgtcgc tcatgatcga gaagattgct cccacacggg acgagcgcct gtttctgatg
      301 ctgtccatct tcatcggcat tctgtcaggc cttcttgtgg tggccttccg catcgccatc
      361 gagtggatca aaatcctgac tctgggatcg gcccctcacg ccggccagta tcggctgctg
      421 ctggttcccg ccattgccgg actggtggtg tccgccctcg ttatctggtt cttcccggcg
      481 gcgcgcggca gcggcgtcaa tcagaccaaa gcggcgctct acgtctacga gggttacatc
      541 tctttccgca ccgttattgg gaaattcctc acctcggccc tggcgatcgg cgcagggcac
      601 tctctggggc cggaagatcc ttcgctgcag atcggcgcgg gcgtcgcatc gatcatcgcg
      661 cgcaacctcc ggctgtctcg ccgccgcctg cgcatgtttg cgccgattgg cgcagctgcc
      721 ggacttgcgg ccgcgttcaa cgccccgatc tcagccatcc tcttcgtcat tgaagaggtc
      781 atcggaagct ggagcgcggc ggtgctcggt tccctggtcc tctccgccat ttccagtgtc
      841 gttgtcgcac gctggttctg gggaccggag ccgatgttcc gcattccctc gatcaccctc
      901 cgcgatcccc gcgaactcat ggcgtatgcc ttgctgggtg tctttggagg attagtcgcc
      961 ccagtcttct ccagcatcct cggctatctt cgcccgcgcc tgcgtgcggt cccgcgctgg
     1021 aacatctttc tccagccggc ggtggccggg ctgctggtcg gcgccatcgg attctttggc
     1081 ttcccccagg tcatgggaac cggctatgac gtcatcgacc aggctctgca cggccagttt
     1141 gtctggcagg tccttctcgc cctcgtgttc ctcaagctga tggccacgac gttctccttt
     1201 tccagcggaa cccccggcgg aatgttcgcg cccacgctct tcatcggagc catgctgggc
     1261 ggagcggtcg gggcctttga gaagcacttc ttcccgcatt taaccggaac cgtgggtgcc
     1321 tattccctgg tcggcatggg ggtgcttttc gcagggttcc tgcgggtccc cctcacctcg
     1381 gtcttcatgg tgctcgaggt cagcgggaac tactccatcg ttcttcccat cattctggcc
     1441 aataccctcg cttttttaat ctcgcgcagc ctccagccgg tgggcatctt cgagatcttc
     1501 acccagcagg acggtctcga cctgccctcg atggaggaac agcgtgagga atcgagcctt
     1561 cacatcgagg acgcactgca accggtgagt gtaccggtgg tggcgggcag cgacacaatc
     1621 cagtcggctt ccgaggcggt ttctgaaacc cactccatgg gttgtatggt tcgcctgagc
     1681 gacggttcat ggtacgcgct gagcgcggac gaactcgcgg gggcaggagc cacgctcact
     1741 ccagaggctc ctgtccagcg cgccctgaag gaggatcggg cgccgcttct gtttcccgat
     1801 atgcctctgg actcggctct gcatcacttc ccacgctggt cgttccttcc ggttctgaat
     1861 cgcgccagcc gcggaaccct ggaaggaatc atcacgatga acgacgttct gaagcgctat
     1921 cgcagtccgg aataacagaa ccccgcttgc atcgaggtcc cctccccact aggctcaagc
     1981 ttcagggatc tccacgccgc atcgcccatg ctgagtctct atcaacgact cattttcggt
     2041 tgtgtactgc tgattgcgct ggtcacagcg gtcagcatgc tggtgcgcac gtcgttcgtg
     2101 caccttgccg ccctggacgc ccaggtgcag gtcgcgcaaa ccgcagtcgc ctcgctcgcc
     2161 tcggcccagg gggccgtggc acacgaggag ctgatcgctg cgcgaattgc cacaggaacc
     2221 gccagcctgc gggaatttcg cgagcaggag ggtcggacgc aggcccttct tcggacggcc
     2281 gttgacgatg tccgggcttt cgatccgagt cttccagtcg atcgtttggt gagcgacaac
     2341 gagcgcctcg cgcagcattc tcccgtcgac cccggtggcc tcacccgttc tttggaggct
     2401 ctgcacgttg agcttcgccg cgatctcgag caattgtccg cccgccgtga ttccaccgtc
     2461 acagcccttc accaccagca ggactggctg cgcgaacgcc tgatcgctgc gtgtggactc
     2521 tcgattgctg cagcgacggt tatctccgca gcgatgctct ttctcgtcat tacacctctg
     2581 cggcgcacag ctcggatcgc caggcgcatc ggccagggtg atctgcatca gcgggtggaa
     2641 tggagatcca aagacgatct cgggacgatt gccaccgagc tcaatcgcct cgctatccgt
     2701 ctgcgtgacc tccgagagac tgaatccggg cgccggcaga tggagttcca gctttccgat
     2761 gccgtggtgc gctcgatctt cgagccggtg atcgttaccg acggcaaagg ccaggtcctc
     2821 aagctgaacc agtctgcaat tgaactgctc ggcggagatt ccgccgacgg cgaagtcgac
     2881 cgcatggtcc tcaccagcac cccaggcggg gataagattc tgagcgcgat ccgggccgcc
     2941 gtatcgatgc agcgtgccat gaccggcgga gagggagaag ccgccctgct tcccatgcgc
     3001 atcggcaagg agcaacggag ctatcgcctg cgtacaacgc cgatgcgtga cagcgaggga
     3061 aaactcctcg gcgcggttac cctgctggaa gacgtcacgg agatcgcaga agtcgaccgc
     3121 ttcaagtcgc ggtttctggc ggtggcttcg caaaaactgc gcgagccgat ccgccggctg
     3181 cgcctcgcgc tttttgcaat aacccagggc cactccggtg aactccgccc attgcaaatg
     3241 gatctggcga ccagtgcagc gcacgaagcc gaacaactgg acgatctcat gctcgacctg
     3301 atcgaggttg ctgaactgga aaccggtcgc cgtcagctgc gcatggaaaa gatccgcccc
     3361 accgacgcgc tgcgggactc cgcggcccga tgccgggatg cggtccgggg caagaacatc
     3421 gacatggaga ttctggcctt cgacgatgtc gcgtatgtgc aggccgaccg gcgcgcgctg
     3481 cgcacgattc tcgacaacct tattacgaac gcactgcgct acacgccgga gggcggtcat
     3541 atccgcatcg gagcgacaga acagcaggac cgggtacagt tcttcgttca cgacaacggt
     3601 cgcggcattg aagccgagcg tttgccgaca atctttggcc gcttcaccgg cgatcccaac
     3661 agcggaacct caggtcttgg cctggccctg gtgcggcgcc tggtggagtc tcagggggga
     3721 gaggtttccg ttgagagccg cctcggctca gggaccacat tccgctttac actccccatc
     3781 gccgtcgccg agccggatcg ccaccctgtg gaggttggat gaccacgcct ccgtccattc
     3841 cgcctgagga ctccacaccg gagccgttac gtcccgtaac gcccgtccag actgaccacg
     3901 aaccgggccg gcttcgcgtc tatctgggcg cggctcccgg cgtgggcaag acctatcgca
     3961 tgcttgagga cgcccacctc atgcggcgac agggagtcga tattgtcatc gggctgattg
     4021 agccccatgg acgcgccgat acggcggcac tgattcaggg tctcgacatt attcccctgc
     4081 aggagattca ataccggtcc gtcacgctgc gcgaaatgaa tatcgatgcg gtccttgcgc
     4141 ggcatccggc atggtgcgtc gtcgacgaac tggcgcacag caatgtgccc gggtcccgca
     4201 atcacaagcg gtatgaagac gtgctcgagc tgctggatgc gggcatcaac gtgatgaccg
     4261 caatgaacgt gcagcatctg gaaacgctga acgacgcggt agcacgctcc gccagtacgc
     4321 agattcgaga aacggttccc gatagcttcc tgcggcgcgc ggacgaagtg gtcaacgtcg
     4381 acatcaccat cgacgagctg cgcgaccgcc ttcgccaggg caagatctac gcgcccgaaa
     4441 aagtggagca ggcgctggcg aacttcttcc gcaagggcaa cctcaacatg ctgcgcgaac
     4501 tggccctgcg aacgacggcg gaacaggtgg gcacagccgc tgccgaatac cgtcgcaacc
     4561 agggtctgga acaggcgccc attccggaaa aggtcatggt ctgcctctct ccccgggcgg
     4621 gaatggagcg gctcatccgc gtgggagccc gcgtcgccgg ccgtctcgcg acgaactggt
     4681 atgtcgtcta cgtggaaact cctgagaccg atcaccgtca tggtgaccca gccgcttccg
     4741 ctcgtctgga agactacgag cgcatggcgc gggatctggg agcccgcatc gtcaagctca
     4801 aggacaggaa agtcgcggat gcgctgatcg aattcgctcg aaaagagaac atctcccatg
     4861 tggtgttcgg gcaatcggcc cgtacgcgct gggacattct gctccgtggc tccatcatca
     4921 atcgcttcct gaatgaaatg cgggatacca ccgtgcaggt ggttccggtt cagaaggaag
     4981 tcagaggccg ggcatgacag acgccgtgga aaccggaggc aatcgatgaa ccgggacgca
     5041 atgctggcgc gaatccgaga ccgcgccgaa cccttcgatc tggtcatcat tggcggcggg
     5101 gcgaccggag ccggaatcgc cgtcgatgct gccagccgcg gcttctcgac tctgctgctg
     5161 gagcgcgagg acttcggcaa gggaacctcc agccgcagca ccaagctggt tcacggaggc
     5221 gtgcgttatc tggagcaggg caatctttcg ctggtgatgg gagcgctgcg ggagcgcggc
     5281 attctgcggc gcaatgcgcc acacctggtc catgatgtgc cctttgtcgt tcccaactac
     5341 tcctggtggg agacgccctt ttacggtatt ggcctgaaga tctacgacct gctggcgggc
     5401 cagtacgggt tcggcaaatc caaactgctc tcgcgcgacg aaactctgga gcggctgccg
     5461 acgctgcgcc aggatggact gcgcggtggc gtgatctatc acgacggcca gttcgacgat
     5521 gcccgcctac tgattcattt gctggcgacg gccgcggact atgacgctac ggtcctcaac
     5581 tatgcgccgg cggtcgagat ccgccgcggt tcggacggat tcgtctgcgg ggttgtcgcc
     5641 gaagatcgcg aatccggaga gcgtttttcg atcgatgccc aggttgtcat caatgccacg
     5701 gggatcttcg ccgatgagac acggcgtatg gcggatgcca atgccgcacc catgatggtg
     5761 ccaagccagg gcattcatct tgtcctcgag ggatccttcc tgcgcggcaa cacggcgatc
     5821 atggtgccgc acacgagcga cggacgggtg ttgttcgcga ttccctggca tggccacacc
     5881 gtggtgggca cgaccgacac atcgatcgac ggcccttctt acgagccgct gccgttcgaa
     5941 cacgagatcg agtttgtgct ggagacagcg agtg
//
LOCUS       DASZYN010000485         6060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_664466, whole genome shotgun sequence.
ACCESSION   DASZYN010000485 DASZYN010000000
VERSION     DASZYN010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664466"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VHE33_12485"
     CDS             <1..715
                     /locus_tag="VHE33_12485"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HVT98315.1"
                     /translation="RQEGRARGWLFVLADGVGGLDRGEVASARTVQVITEGFVEAPEN
                     TSLLGLLPRLIQHANAAVHDEGFARERRGRLMASTVVACAVRHDQAYVAHVGDSRCYH
                     VRGREITAVTQDHTIAGEQSRQGLISSEDAEESEVGHVLSRALGPELYVTADSTTVSL
                     RAGDFLVLCCDGVYAGVDAPDMVRILAQPKPAQALADELVQTAVENDGSDNTTAQVIA
                     IRSVEAMAMYRGRLYSRLS"
     gene            730..1725
                     /locus_tag="VHE33_12490"
     CDS             730..1725
                     /locus_tag="VHE33_12490"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HVT98316.1"
                     /translation="MEATTPVTDSPGSLVEAGQQLDHYRLDALVAESPVTRTFRATDL
                     RDGRVVAIKVPQPAMEADPLALDRFRREEEIGASLNHPDVKRIFHEPHRSRLYMVLEW
                     CEGRSLDQILKESGKLPPQRAIHIARGILRALEYIHQHGVVHRDLEPEHILVDDHDRI
                     KLIDFGVASRQGARRLTFTSLSQAMGTPDYISPEQVKGKRGDARSDLYAVGIMLWQMV
                     MGRTPFTGRSPVAAMNARLTRNPPPIRLPDFPLAAQLQEVICRATEREPRNRYASAHE
                     FHTDLEHLDQVGVAHRPELGQTGGGSKLLTKVLLYSAIVVIPIILFVVMLLFSRH"
     gene            2021..2413
                     /locus_tag="VHE33_12495"
     CDS             2021..2413
                     /locus_tag="VHE33_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98317.1"
                     /translation="MNSQALFLELLVSGFFAIACFKARAAVEPGQVALRPRDLLRLTD
                     RLERLSRSRWQWFAMVGILVLVRMQAGVPLAVELTAAAQFVLFLALPIAHPAKSGGVA
                     AGVSSGRRSVGAAQKRQRAMPAASHGLD"
     gene            2500..2889
                     /locus_tag="VHE33_12500"
     CDS             2500..2889
                     /locus_tag="VHE33_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98318.1"
                     /translation="MAATKQQGQWFGLFLIGLTVTCAGIYDLSSGTGKIALALGLVVL
                     VVSFLRFLGLKPLEGKVALGAQPAGMKAVGVLVTAAGWLLALFGLHLASGVGGRMVFA
                     ILGLGVSLVGVVLILPAACNKNAIWKA"
     gene            2915..4561
                     /locus_tag="VHE33_12505"
     CDS             2915..4561
                     /locus_tag="VHE33_12505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HVT98319.1"
                     /translation="MKKTLPQKFLLLAVVLMWSLGGLAHAQTATADSELKAASAAAPT
                     TTTQAAGDPGGSTTGTVNDVPVSDAKAGVTVADVGNQVGQNKIAINFTWTLITGFLVM
                     FMQAGFAMVEAGLCRVKNANHTYMMNFTVYACGLFAYWLIGFAIQMGGAAGNGNLGGL
                     QPLNGEHTMSLFGKTWGLWGTQGMLLSGHTYDVGVMVIFLFEMVFMDTALTIVTGAAA
                     ERWKFLTFCVSSVLMGAFTYPIFGNWAWGGGWLAALGSNLGLGKGYCDFAGSGVVHAV
                     GGLTALAVALIIGPRIGKFNRDGSPNAIIGHDISAVLIGCFILAFGWFGFNPGSTLGA
                     SAAGNLRIGSIAVNTMLAGCTGTFGAMLYMWALKGKPDASMSANGLLAGLVAITAPSG
                     FVNPVGSAIIGAIAGVLVCLSVAFVESKMKIDDPVGAISVHGTCGLWGVLSVGLFADG
                     KSNYGGAWNGVTGSVTGLFYGDASQLVAQIIGIGTLIGFVFTFSFALNWVLDVFLGQR
                     VSAETELEGLDLPEMGQLGYPEFVFRPEPAFMHVEAAAVA"
     gene            4654..5232
                     /locus_tag="VHE33_12510"
     CDS             4654..5232
                     /locus_tag="VHE33_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98320.1"
                     /translation="MSALATSVAGQETELPLVQETAQLAFPPAIPAGAQQQLLRMFRD
                     PYLLEAPDRADLIADLRDAVALAPEIPEVRVLLGMALCVDLQAQEAMEELRESVRLAP
                     DSFIAHLKFGELLMRLRICDQAAEETRMAAELATSSLQSELARRQAATIRTMRREGIE
                     RGGYGKLLSVVSRVPKLLSRVHPGRRRAALEA"
     gene            5336..>6060
                     /locus_tag="VHE33_12515"
     CDS             5336..>6060
                     /locus_tag="VHE33_12515"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HVT98321.1"
                     /translation="MPPILQLALLIAILLPAGKIVASICSRVGIPPIIGELMVGILAG
                     PGLLNLLHLRLFSGTSGSSAFMLLAQVGGLVLMFIAGFETDIDRMKEAGVTAFLVALS
                     GVIWPFMLGAAAGHLFGLSWSVSLFLGGALTATSVSISARTLMDAGRMASPEASVILG
                     AAVIDDVMGLFVLAFLTASSAGSATETFGAAPALSTWVDHHWAFAAQHSLLVQMIFIV
                     LSVALFFVLGYLPAKRWLDALIL"
BASE COUNT          993 a         1786 c         2043 g         1238 t
ORIGIN      
        1 ccggcaggaa ggtcgcgcgc gcggctggct gttcgtgctt gccgacggag tgggcgggct
       61 ggacaggggc gaggtggcgt cggcgaggac ggtgcaggtg atcaccgagg gctttgtcga
      121 ggcgccggag aatacgtcgc tgctgggcct gttgccgcga ctgatccagc atgccaatgc
      181 ggcggtgcat gacgaaggct ttgcgcggga acggcgcggc cggctgatgg cttcgacggt
      241 ggtagcctgt gccgtgcggc acgatcaggc gtatgtcgcg catgtcggcg attcgcggtg
      301 ttatcacgtg cgtggccgcg aaatcacggc ggtgacgcag gatcacacga ttgccgggga
      361 gcaaagccga caggggctca tcagctccga ggacgcggag gagtcggaag ttgggcatgt
      421 gctgagccgg gcgctggggc ctgaactcta cgtgaccgca gacagcacca cggtgagtct
      481 tcgcgcaggc gattttcttg ttctttgctg cgacggagtc tatgccggcg tggatgcgcc
      541 ggacatggtg cggattcttg ctcaaccgaa accagcgcag gctctggctg atgaactggt
      601 tcagaccgcc gtggagaacg acggctcgga caacacaacg gcgcaggtga tcgccattcg
      661 ttcggtggaa gccatggcga tgtaccgcgg gcgcctctac tcacgcctca gctgagggga
      721 gcagcgccga tggaagccac aaccccggta acggattccc caggcagcct ggtcgaagcg
      781 ggccagcaac tggaccacta tcggctcgac gcgctggttg ccgagagccc ggtgacgcgg
      841 acgtttcgag ctactgatct gcgcgacggc cgggtggtgg ccatcaaggt tccgcagccg
      901 gcgatggaag ccgatccgct ggcgctcgat cggtttcggc gcgaggaaga gatcggcgca
      961 tcgctgaatc atcctgacgt gaagcggatt tttcacgaac cgcatcgctc gcggctgtac
     1021 atggtgctgg agtggtgcga aggccgttcg ctggaccaga tcctgaagga atcgggcaag
     1081 ctgccgccgc agcgcgcgat tcacatcgcc aggggcatcc tgcgggcgct cgagtatatc
     1141 caccagcacg gcgtggttca ccgcgatctg gagccggagc acatcctggt ggacgatcac
     1201 gatcgcatta agctgatcga ctttggcgta gcttcgcgcc agggggcccg ccggcttacg
     1261 tttaccagcc tgagccaggc gatgggcact ccagactaca tttcaccgga gcaggtgaag
     1321 ggcaagcgcg gggatgcgcg ctccgatctg tacgctgtcg gcatcatgct gtggcaaatg
     1381 gtgatggggc gcacgccgtt caccggacgc tcgccagtcg ctgcgatgaa cgcgaggctc
     1441 acacgcaatc cgccgccgat ccggctgcct gactttccgc tggcggcgca gctgcaggag
     1501 gtcatctgcc gtgcgacgga gcgtgagccg cggaatcgct acgcctcggc gcacgagttt
     1561 catacggacc tggagcatct ggatcaggtg ggcgtggcgc accggccgga actgggccag
     1621 acgggcggcg gaagcaagct gctcacgaag gtgctgctct actcggcgat tgtggtgatt
     1681 ccgattattt tgtttgtcgt catgttgctg ttctcgcgcc actgagagcg gcgccggcta
     1741 aggccgctac aagtgcatca accgattgtt tccgaatatc cgacaaatgc atgatggcag
     1801 gcaggatacc ttcctgcgat attcgaaaat gagcgcgagt ttgcacgcgc gccgggccga
     1861 tgattttgcc ctctctgagg gtctttatga aatcctcatt gcccccatgg tttcctggta
     1921 gcacaacgga aagctgaata cccgagggat gcaagggccg atggaagacc cctcggtaga
     1981 gctgtatccg caggtccgag attgctaccg gaggatgcaa atgaatagcc aggcactgtt
     2041 tctggaactg ctggtttcgg ggtttttcgc gattgcgtgc ttcaaggcgc gagccgccgt
     2101 ggaaccgggg caggttgcgt tgcgcccccg cgacctgctc cgcctgaccg atcgcctgga
     2161 gcgtttgagc cgttcgcgct ggcaatggtt cgcgatggtc ggcatcctgg tgctggtccg
     2221 catgcaggcg ggtgttcctt tggcggtgga actgaccgcg gccgcccagt ttgttctgtt
     2281 tcttgctttg cccattgcgc atccggcaaa gtccggcggt gttgccgcag gggtctcttc
     2341 gggacggcgc agcgtgggcg cggcacagaa gcgtcaacgc gcgatgccgg ccgccagtca
     2401 cggactggac taatcgatct tgcctctccc gctcgtcctg ccctgggcaa cgctccgggc
     2461 gtgtagtcac acaacaaccc cattcaggga ggaatagaga tggcagcaac gaagcaacag
     2521 ggccaatggt tcggcctgtt tctgatcgga ctgacggtca cctgtgccgg catttacgac
     2581 ctgtcgagcg gcacgggcaa gatcgcactc gcgctgggtc tggtggttct cgtcgtatcg
     2641 ttcctgcggt ttctcgggct caagccgctg gaaggcaagg tcgcactggg cgcgcagccg
     2701 gcggggatga aggcggtggg cgttctggtg acggccgccg gctggctgct ggcgctgttc
     2761 ggacttcacc tcgcttcggg agtcgggggg cgcatggtgt tcgcgatcct cggcctgggg
     2821 gtgagcctcg tcggcgtggt gttgattctg cccgccgcct gcaacaagaa cgccatctgg
     2881 aaggcctaac ccacacgaca tggagcccta ttcgatgaag aagactcttc cacagaagtt
     2941 cctgctgctg gccgtcgtgc tgatgtggtc ccttggcggc ctggcccacg cgcagacggc
     3001 gaccgcggac tccgagctga aagctgcgtc ggctgccgcg cccacaacaa ccactcaggc
     3061 cgcgggcgat ccgggcgggt ccacgacggg tacggtcaac gatgttccgg tctccgacgc
     3121 caaagccggc gtgacggtcg ccgatgtggg caaccaggtc ggacagaaca agattgcgat
     3181 caactttacc tggacgctga tcaccggctt cctggttatg ttcatgcaag ccggcttcgc
     3241 gatggtggaa gcgggccttt gccgcgtgaa gaacgccaac cacacgtaca tgatgaattt
     3301 caccgtgtat gcgtgcggac tgttcgcgta ctggctcatc ggattcgcga ttcaaatggg
     3361 aggcgcggcc ggcaacggga acctgggcgg gctgcagccg ctgaacggcg aacacacgat
     3421 gagcctgttc ggcaagacgt ggggcttgtg gggcacgcag ggcatgctgc tctcgggcca
     3481 cacctatgac gtaggcgtga tggtcatctt cctgttcgag atggtcttca tggacacggc
     3541 gctgaccatc gtcacggggg ccgctgcgga acgctggaag ttcctcacct tctgtgtgtc
     3601 gtcggtgctg atgggtgcgt tcacctatcc catcttcggc aactgggcat ggggcggcgg
     3661 ctggctggcg gcgctgggca gcaaccttgg actgggtaaa ggctactgcg attttgccgg
     3721 ctcgggtgtg gtgcacgcgg tgggcgggct gacggcgctg gcggttgcgc tgatcatcgg
     3781 gccgcgcatc ggcaaattca atcgcgatgg ctcacccaac gcgatcatcg gtcacgacat
     3841 ttcggcggtg ctgatcggct gcttcattct ggcgttcgga tggttcggat tcaacccggg
     3901 cagtacgctg ggcgcctccg ccgcgggcaa tctccgtatc gggtcgatcg cagtcaatac
     3961 catgctcgcc ggctgcaccg gcaccttcgg cgccatgctc tacatgtggg cgctcaaagg
     4021 gaaaccggac gcttcgatgt cggcaaatgg cctgctggcc ggtctggtcg cgattactgc
     4081 gcccagcggc ttcgtgaacc ccgtaggctc ggcgatcatt ggcgcgattg ccggcgtgct
     4141 ggtctgcctg agcgtcgcgt tcgtcgaaag caagatgaag atcgacgatc cggtcggcgc
     4201 gatctccgtc cacggaacct gcggcctgtg gggtgtgctc tcggttgggc tgttcgccga
     4261 tgggaagagc aactatggcg gggcctggaa cggtgtcacc ggttcggtta ccggcctgtt
     4321 ctacggggac gcgagccagc tggttgcgca gatcatcggc atcggtacgc tgatcggctt
     4381 cgtcttcact ttcagcttcg cactgaactg ggtactggac gtcttccttg gccagcgcgt
     4441 gtcggcggag acggaactgg aaggtctgga cttgccggaa atgggtcagc tcggctatcc
     4501 ggagttcgtc ttcagaccgg agccggcctt catgcatgtt gaagccgccg cggtcgctta
     4561 acggtagccc tctcctgcag cagggagtgt cctgagcact ccctgctgtg ccccttcaac
     4621 acagatggtt gttaacggac ggagatctga atcatgagtg ctcttgccac ttctgtcgca
     4681 ggccaggaaa cagaattgcc gttggttcag gagacagcgc aactggcctt cccgccggcg
     4741 atcccggcag gcgcgcaaca acagctgctg cgcatgttcc gtgatccgta cctgctggag
     4801 gcgcccgacc gcgcggacct cattgccgat ttgcgcgatg ctgtggcgct cgctccggag
     4861 atccccgagg tacgcgtgct tctgggaatg gcgctctgcg tcgatctgca ggcgcaggaa
     4921 gcaatggaag aattgcgcga atccgttcgg ctggcgccgg acagctttat cgcccatctc
     4981 aaattcggcg agctgctgat gcggctgcgc atctgcgacc aggcggcgga agaaacccgc
     5041 atggccgctg agctggcgac gagttcgctg cagtcggagc tggcccgccg ccaggctgca
     5101 accattcgca cgatgaggcg cgagggcatc gaacgcggcg gatacgggaa gctgctttca
     5161 gttgtcagcc gggttccgaa actcctgtcg cgggttcatc cgggccgtcg gcgcgctgct
     5221 ctcgaagcct agccacccat aacggcctgc tgctggaggg ctcgcgggcc gagaagggaa
     5281 gtcccttgga tcttcagtcg aaggcaattc gcttaggagt tcggtccccg cactgatgcc
     5341 tccgattctc caactcgcac tgctgatcgc gatccttctg cctgcgggca aaatcgtggc
     5401 ctctatctgt agccgggtgg gaattcctcc catcatcggc gagctgatgg tcggcatcct
     5461 cgccggtccc ggccttctca atctgctgca ccttcgtttg ttcagcggaa ccagcggctc
     5521 cagcgccttc atgttgctgg cgcaggtcgg cggcctggtg ctgatgttca ttgccggctt
     5581 cgagaccgac atcgatcgga tgaaagaagc gggcgtaacg gcgtttctgg ttgcgctgtc
     5641 gggcgtgatc tggccgttca tgctcggggc tgccgccggc cacctgttcg ggctctcgtg
     5701 gagtgtctcg ctctttctgg ggggcgcgct cacggccacc agcgtgtcga tttccgctcg
     5761 cactctcatg gatgccggcc gcatggcatc gcccgaggcc tcggtcattc tgggtgccgc
     5821 tgtcattgac gacgtgatgg ggttgtttgt tctcgccttt ctcacggcgt cgtcggctgg
     5881 ttcggccacg gagacgtttg gggcggcccc tgcgctgtca acgtgggtcg atcatcattg
     5941 ggcgttcgcc gcgcaacact cgctgctggt gcagatgatt ttcatcgtgc tcagtgtggc
     6001 gctgttcttt gtgctggggt atctgccggc gaagcggtgg ctcgacgcgc tcatcctgca
//
LOCUS       DASZYN010000486         1605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_599205, whole genome shotgun sequence.
ACCESSION   DASZYN010000486 DASZYN010000000
VERSION     DASZYN010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599205"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VHE33_12520"
     CDS             complement(<1..950)
                     /locus_tag="VHE33_12520"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98322.1"
                     /translation="MPDYIYLLENRLSPAQQNALRHVRDAARDAGMTVFLTGGAVRDL
                     TSGFPVRDLDFSVQGNALKLKKYMEKAGGEFWGDHEPSRTLYFWFPGSVRVEVSSSRR
                     EEYPKPGKPVYHWAPILEDLRRRDFTANAMALSLNEGSWGLLLDPLNGVADIEARHVR
                     LASNYGFIEDPSRMLRAIRFAARTGWQMDERTMARYQNAKTEGLMEQISAFARGYELE
                     EIAHEEDPLRTLRALEAEGWMKVLCPAWISAKADVKGLEELNKILIKLLMLGVSPDPS
                     AAHIELLTAKMQPKDVANLKRQFPRRGFVEQWNQLEQSAK"
     gene            1190..>1605
                     /locus_tag="VHE33_12525"
     CDS             1190..>1605
                     /locus_tag="VHE33_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98323.1"
                     /translation="MSSTRKKAIFRKFSRDWVAGYLPATSFVREGHIEMLDLDGKVIA
                     LAAHDVKWICYVRDFNSGELNNPERLVRKTFAGRPRGDGLFVRARLKDGDLIEGLAAN
                     DVTLVGSEGVFLTPPDLRSNTQRLFIPRTSIEELEVV"
BASE COUNT          325 a          532 c          427 g          321 t
ORIGIN      
        1 tccttcgcgc tctgctccag ctgattccac tgctccacaa aaccgcgccg cggaaactgc
       61 cgcttcagat tcgccacgtc cttcggctgc atcttcgccg tcagcaattc aatatgcgcc
      121 gccgacggat ccgggctcac gccgagcatc agcagcttga tcagaatctt gttcagctcc
      181 tccaaaccct tcacatcggc cttggccgag atccaggccg ggcacagcac cttcatccac
      241 ccctcggcct caagggcgcg cagagttcgt aacggatcct cttcgtgcgc aatctcctcc
      301 agttcgtagc cgcgagcgaa tgccgaaatc tgctccatca ggccttcggt cttcgcgttc
      361 tgataacggg ccatcgtgcg ctcgtccatc tgccaccccg tccgggcggc gaagcggatc
      421 gcgcgcagca tccgcgaggg gtcctcgata aatccgtaat tgctcgccag ccgaacgtgg
      481 cgtgcttcga tgtcagcaac accgttcagc ggatccagca gcagtcccca cgagccttca
      541 ttcagcgaca gagccatcgc gttcgccgtg aagtcgcggc gtcgcaggtc ctcaaggatt
      601 ggcgcccagt ggtaaaccgg cttgcccggc ttgggatatt cctcccggcg agagctggaa
      661 acctcgaccc gaacgctgcc cgggaaccag aagtagagtg tccgggacgg ctcatggtct
      721 ccccagaact cgcctccggc tttctccata tatttcttca gtttgagagc atttccctga
      781 accgaaaaat caaggtcgcg gaccgggaaa ccactggtca gatcgcgcac cgcgccaccc
      841 gtcaggaaga ccgtcatgcc cgcatcgcgg gccgcatctc gaacgtggcg aagggcgttt
      901 tgctgggcgg gggaaagacg attttcaaga agataaatgt aatcaggcat gcgtgaggta
      961 ttctgcgtgt tgatgccgga agcttccgac tccggtcccc ggacagctgt ccgtaactcc
     1021 agtggaagga atgagttacc ggacacacta aaggcaatac aaactatcaa tgtaacacaa
     1081 aagtgtgcac tttgggcggt gcagtttgca caaattttga tgcgtcccgc cggaatgtgc
     1141 aacaatcctg ctttcccttc agcaaccacc tcggaaacct ctgaaacgca tgtcttcgac
     1201 ccgaaaaaag gcgatcttcc gcaagttctc tcgggactgg gtcgctgggt atctgcccgc
     1261 cacctccttc gtccgcgaag gccacatcga gatgctcgac ctcgacggca aggtcatcgc
     1321 cttggccgcc cacgatgtga agtggatctg ctacgtccgc gatttcaatt caggagagct
     1381 gaataacccc gagcgcctcg tccgcaaaac cttcgccggc cgcccccgcg gtgatggcct
     1441 cttcgtccgc gctcgcctga aagacggtga cctcatcgag ggcctggccg ccaacgacgt
     1501 caccctggtc ggcagcgagg gcgtcttcct caccccgccc gatctgcgct cgaacaccca
     1561 gcgcctcttc attcctcgca cctcaatcga ggagctggaa gtggt
//
LOCUS       DASZYN010000487         5682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1117339, whole genome shotgun sequence.
ACCESSION   DASZYN010000487 DASZYN010000000
VERSION     DASZYN010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117339"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="VHE33_12530"
     CDS             complement(<1..1321)
                     /locus_tag="VHE33_12530"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98324.1"
                     /translation="MESNALLNLGFASLGGEHYDEALDWSRLAYQEAESLGAEDLAQR
                     ASGNIGWAYFQLGDSDRAQELFADAERRAAALGNLREQLRWLTTQGVVAQTNHDLAKA
                     SEVYLQTLNLSRQIDSKEDVVNSLESLAHVSIEQGQLNNAESYLRQVDPLIRANGNHL
                     DALDVTLARGRIAAARRQDQQAESLFRQVDADPASQITMRLGAEHELARLFEVEGRGE
                     DARKEYVTALGTFESARAAIKTEDSKLPYFANATPIYDDSIRLLLSEGKIEEALMEAD
                     QSRARTLEQGLGVMGGNASVKEAALRPESIAGKSGATLVFYWLGEKQSWLWAITRQKT
                     TVVPLPPRAEIARAVERYRQALLGPEDPLRAHNADGLALYRVLVAPAKDLIPPSGKVI
                     VLCDGDLSKLNFETLLVDSPSPHYWIEDADVVSAPSLRMLAIAKPARA"
     gene            complement(1861..2730)
                     /locus_tag="VHE33_12535"
     CDS             complement(1861..2730)
                     /locus_tag="VHE33_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98325.1"
                     /translation="MPEESTPTPTPTKREHVYHAQAYALDGDLHRPLKTPVKPQSYVK
                     VPDGGGYLSERSVDYRLEGIFSFRHAYSQVAGNRSLKPGGGWVTLATSVMEGFNVLDV
                     VTADRIVAQISTEYPLDGYVPEVTFLGTRFENLKIAGHHVDVKLDTGFLGSKPANDGI
                     YHGDKGFRERVNEQRSRILKGKSLPDEIGQRYNQLPSNAVTGETIEWSLVQHAEGGYP
                     GTSFGHAIDIPHFGKVYLGVVRLQHGTYQTSVPGDHPSTTISLTMVEMVMGCIGHGKA
                     GGGNAVVNGTSAP"
     gene            complement(2765..3463)
                     /locus_tag="VHE33_12540"
     CDS             complement(2765..3463)
                     /locus_tag="VHE33_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HVT98326.1"
                     /translation="MDHGEAVELMATERYLLGELSPEQREAFEAHFFECYECALDIRA
                     EAAFLKEAKVQLPQMTQSAAPVEGRSAPVKTQTKRRDWFGWMRPVWAVPAFAALLVVI
                     GYQNLATIPGLRSAADAPRVTPWSTLHVGTRAGAHTAVTADRKNGTTLLIDVPNDGTY
                     TSFGFALENPEGKQTWMETVKAPANGDGTISLSIPGRGLQEGAYTLTIAGITPQGSRT
                     EIERQLLDVRFNEQ"
     gene            complement(3522..4103)
                     /locus_tag="VHE33_12545"
     CDS             complement(3522..4103)
                     /locus_tag="VHE33_12545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVT98327.1"
                     /translation="MQFYAFDAGYVEGLCAGDLQTQEHFVGYFTELLHLKLRSRLHSR
                     QAIEDVRQETFARVLATLRRENGLRQPDRLGAFVNTVCNNVLFEHYRANSRSESLDEE
                     DRPELPASGASAHDIAAANQLKGKVREILAELSPRDRGVLKAIFLDERDRDDVCREFG
                     VDGEYLRVLLFRARQNFKAEYLKRMGGGPDAAR"
     gene            4744..>5682
                     /locus_tag="VHE33_12550"
     CDS             4744..>5682
                     /locus_tag="VHE33_12550"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVT98328.1"
                     /translation="MKPTFAIRGTITAALLLLAAGAALAQTPGTGAISGIVTDPQNHT
                     VGSADVLAENEATHIVRSATTGADGEFRIPLLVPGRWTVTVRAPGFAPNATHEVEVTV
                     SETRSLTVQLAVAADAGTTVHVSGRAEDVQAESSTLGGVVDETAIQTLPLASRNYTQI
                     LGLSPGVVVDLPNAAQLGSGSQNVASDGATPTANNIQFNGIDANNLAENSAAIAETSE
                     VGVAIPAPDTIQEFRVQTANFDAAYGRGSGANVDLVSKSGANNFHGSAWEFVRNNLFN
                     ANDFFSKLSGQPKPDLKQNQFGAAAGGPILHDRTFFF"
BASE COUNT         1095 a         1919 c         1537 g         1131 t
ORIGIN      
        1 tggcgcgcgc gggcttggcg atcgccagca tccgcaagga tggcgccgag accacgtcgg
       61 catcttcaat ccagtagtgc ggtgacgggc tgtcgacgag cagggtctcg aaattcagct
      121 tgctcagatc gccgtcgcac agcacgatca ccttcccgct cggcgggatc aggtccttcg
      181 ccggcgccac cagcacgcgg tacagcgcca ggccatcagc gttgtgcgcc cgcagcggat
      241 cctccgggcc aaggagcgcc tgccgatagc gttccacggc gcgcgcgatc tccgctctgg
      301 gcggcagcgg aacgacggtc gtcttctgtc gcgtgatcgc ccacagccac gactgctttt
      361 cgcccagcca gtaaaacacc agcgtcgcgc cggacttccc tgcaatcgac tctggacgca
      421 gggctgcctc cttcaccgag gcgttgccgc ccatcacgcc cagtccctgc tccagtgttc
      481 gcgctctgct ttgatcagct tccatcagag cttcctcgat cttgccttcc gagagcagca
      541 gacggatgga atcgtcatag atcggtgtgg cattcgcaaa atacggcagc ttggaatctt
      601 cggttttgat tgccgcgcgc gctgactcaa atgtgcccag cgcggtgaca tattccttcc
      661 gcgcatcctc tcctcgtcct tcgacttcaa aaagccgggc gagttcgtgc tcggcgccga
      721 ggcgcatcgt gatctggctc gccggatcgg cgtccacctg gcggaacagg ctctcggcct
      781 gctgatcctg ccgacgcgcc gctgcaatcc gcccccgcgc cagcgttaca tccagcgcgt
      841 cgaggtgatt gccattggcc cggatcaggg gatcgacctg gcggaggtag gattcggcgt
      901 tgttgagttg tccctgttcg atggagacat gagcaagact ctccagcgaa ttgacaacat
      961 cttccttact gtcgatctgg cgcgaaagat tcagcgtttg aaggtatact tccgatgctt
     1021 tggcgagatc gtgattcgtt tgtgccacga caccctgcgt tgtcagccat cgcaattgtt
     1081 ccctcaagtt tccgagcgcc gccgcacgcc gctctgcgtc agcaaacaat tcctgcgctc
     1141 tatcagaatc tccgagctga aaatatgccc atccaatgtt gcccgacgct cgctgagcaa
     1201 ggtcctctgc gccgaggctc tccgcctcct gataagcaag acgcgaccag tccagggctt
     1261 cgtcgtagtg ctcaccaccg agggacgcga agccaagatt cagcagtgca ttggattcca
     1321 tccaatgttg tttgttgtct cgcgcgaacg acagacttgc cagaaagaaa cgtctcgcct
     1381 cggaaattct cccgtgtgcc attgcagaaa ttccgcgcgc tctgagtacg ccaccacatt
     1441 ctacgagcgt ctgcacggca caaagggatt cggccttaga gagtctttcg tcagcagcag
     1501 aaaattgctg cgtgttgatg aaggaaacgc tctcaagtgt cagtgcctcg acgcgctccg
     1561 caacatcctc cggtggcaca tttgagccga cggccaggtg cagtgcccct tcatatagcc
     1621 cacgccacac catcgcctct gcggcgagca aacgaaatcg gcgagcccac gctggactcc
     1681 tgacacgaaa ctgccggtat ccagcgagag cttcggattg tgccttatcc aggtgcccct
     1741 gtaagaaaat ctgaaggacg tggttgtaat cgctatgcgg atcgagctga cgggagtcct
     1801 gcaagggagc cagtacaacg aacagcgcga aactcagagc aacgagaaca aacgggcgcc
     1861 ttacggtgcg ctggtgccgt tgaccaccgc gttgcctccg cccgcttttc cgtggccgat
     1921 gcagcccatc accatctcca ccatggtcag gctgatcgtc gtcgacgggt gatcccccgg
     1981 caccgacgtc tggtacgtcc catgctggag ccgcaccacc cctaaataca ccttgccgaa
     2041 gtggggaata tcgatcgcgt gcccaaagga ggtcccaggg tagccaccct ccgcgtgctg
     2101 gaccagcgac cattcaatcg tctcccccgt tacggcattc gatggaagtt gattgtagcg
     2161 ctgcccgatt tcgtcaggaa gactcttccc tttcagaatt cgcgaacgct gctcgttcac
     2221 gcgttcgcga aatcccttgt cgccgtgata gatcccatcg ttggccggct tgctgcccag
     2281 aaaacccgtg tcgagtttta cgtcgacgtg atgtccggca atcttgagat tctcgaaccg
     2341 cgttcccagg aacgtgacct caggcacata tccatccagc ggatattccg tcgaaatctg
     2401 cgccacgatc cgatcggcgg tgaccacatc gagaacgttg aagccttcca tcacagaggt
     2461 ggccagcgtc acccaaccac cacccggctt caggcttcga ttccccgcca cctgcgaata
     2521 cgcatgccga aaggagaaga ttccctccag gcggtagtcc accgagcgct ccgataaata
     2581 tcctccgccg tcgggcacct tcacgtacga ctgcggtttc accggcgttt tgagcggccg
     2641 atgcagatcg ccatcgaggg catacgcttg tgcgtggtaa acgtgttctc tcttggtggg
     2701 cgtgggggta ggtgtgctct cttcaggcat ggaacttgcg cgtgctcact ttccgggagg
     2761 agttttactg ctcgttgaag cgaacatcca gcaactgccg ctcgatttcc gtgcggctgc
     2821 cctgtggggt gatgcccgca atagtaagcg tataagcacc ctcctgcaac ccccgaccgg
     2881 gaatggacaa cgaaatcgtc ccatccccat tcgccggcgc ctttaccgtc tccatccagg
     2941 tttgctttcc ttccggattc tcgagcgcga agccaaacga tgtgtaagtc ccgtcgttgg
     3001 gcacgtcgat cagcagcgtc gtgccgttct tccggtccgc tgttactgcg gtgtgcgccc
     3061 cagccctcgt tcccacgtgc agcgtcgacc acggtgtgac acgtggcgcg tccgccgctg
     3121 accgcaaccc cggaatcgtc gccagattct gatagccaat cactaccagc agcgccgcaa
     3181 acgccggcac ggcccacacc ggacgcatcc agccgaacca gtcgcgtcgc ttcgtttggg
     3241 tctttacagg cgcagatcgg ccctccaccg gcgccgccga ttgcgtcatt tgcggcagct
     3301 gtaccttcgc ttccttcaga aacgctgctt ctgcgcgtat atccagcgcg cactcataac
     3361 actcgaagaa atgcgcttcg aacgcctccc gttgttcggg gctcagctct ccgaggaggt
     3421 accgttctgt cgccatcagc tccacagctt cgccatgatc catggcctcg gtctcccttc
     3481 tcgctccaga aaatagtgcg ccgccctgct gaaacgtttc actatctcgc ggcatcagga
     3541 cctccgccca tccgtttcag atactcggct ttaaagttct gcctggctcg aaacagcagc
     3601 acgcgcagat actctccatc caccccaaac tcccggcata cgtcgtcgcg atcacgctcg
     3661 tccaggaaaa tcgccttcag gaccccgcga tcccgcggcg acagctccgc caggatttcg
     3721 cgcactttcc ccttcagctg attcgcagcg gcgatgtcgt gcgcgctggc gccggatgcc
     3781 ggcaactccg gccgatcttc ctcgtcaaga gattcactgc gcgagttggc ccgatagtgc
     3841 tcgaacagca cattgttgca gaccgtgttg acgaatgcgc ccagccgatc cggctgtcgc
     3901 agcccgtttt ccctgcgcaa ggtggccagc acgcgcgcga aggtctcctg acgcacgtct
     3961 tcgatggcct gccgggagtg cagccgcgag cgcagtttca gatggagtag ttcggtgaaa
     4021 tacccgacaa aatgctcttg tgtctgaagg tcgcctgcgc aaagaccttc gacatagcct
     4081 gcatcgaacg catagaactg caacgcgggc ctcgcaaacc tgggaggacg caagtatagc
     4141 atcggccccg gcgccggctg gcgcactcca tgctcgcgcg agaccggcct gcgcggtgtt
     4201 gccccacctc tcggcgcgcc tgaatttggc acaacacgtg cgctcatgcc gtcaccccgc
     4261 accttcttct ccccatctgg cgagcagcgc taaggtcagc ccgccagtgg agcagaagtc
     4321 cattccagcc gctgctcaga cagatggaca gggggagaga gaactcaggg cagatgcagt
     4381 tgcaccagga aagcaggatc cgggaaagat tccagcacgt cggtcgcatc atcgcaaact
     4441 ccgattgcgg agcccggccc cgtgggtcaa cggtgggccc cgctggtcag gatttgttct
     4501 gaggattcgc cgcgctcgtt ggcgtcattc gatcctctcg accggatagt gcatctccac
     4561 cgccctccgt ttcacgatcg ggccattcat cgccgtgtca ttgaaatgtc ttcgttcagg
     4621 agaagtctgc cgcccgatgc cccctacaca catcggtaca aagttgtgac aaaggcctaa
     4681 caaatggcat ttctcgcccc cgcatactcg cccccatagc aacaggtttt gggagacgac
     4741 cgtatgaaac ccaccttcgc gatccgcggg acgatcaccg ccgctctgct cctcctcgcg
     4801 gccggcgccg ctctggcgca aactcccgga accggagcca tctccggcat cgtcaccgat
     4861 ccgcagaacc acactgtcgg cagcgccgat gtcctcgccg aaaacgaagc gacccacatc
     4921 gttcgctccg ccaccaccgg cgccgacggc gaattccgca tcccgctgct ggtgccgggc
     4981 aggtggaccg tgaccgtccg cgctcccgga ttcgccccga acgccacgca cgaagttgag
     5041 gtaaccgtta gcgaaacgcg ctccctgact gtgcagctcg ccgttgccgc cgacgccggc
     5101 accaccgtcc acgtttccgg tcgggctgaa gatgttcagg ccgaaagctc cacccttggc
     5161 ggcgtggtcg acgaaaccgc gattcagacc cttcccctcg ccagccgcaa ctacacccag
     5221 atccttggcc tctccccggg tgtcgttgtc gatctgccga atgctgcgca gctcggctcc
     5281 ggcagccaga acgtcgcctc tgacggagca acgcccaccg cgaacaacat ccagttcaac
     5341 ggcatcgatg ccaacaacct cgcggagaac tccgctgcca tcgccgaaac ttctgaagtt
     5401 ggcgtcgcca ttcctgctcc cgacacgatc caggaattcc gcgtccagac ggccaacttc
     5461 gacgccgcct atggtcgcgg cagcggggcc aacgtcgatc tcgtcagcaa atccggcgca
     5521 aacaacttcc acggcagcgc atgggaattc gttcgcaaca acctcttcaa cgcgaacgat
     5581 ttcttctcaa agctaagcgg ccagcccaag cccgacctta aacagaacca gttcggcgcg
     5641 gccgcaggtg gaccgatcct tcacgaccgc accttcttct tc
//
LOCUS       DASZYN010000488         6419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_214904, whole genome shotgun sequence.
ACCESSION   DASZYN010000488 DASZYN010000000
VERSION     DASZYN010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214904"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1073
                     /locus_tag="VHE33_12555"
     CDS             183..1073
                     /locus_tag="VHE33_12555"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HVT98329.1"
                     /translation="MMIFFYIALSFTVFFVITLLCAPFLLRPSVQARRILDVVTSDRP
                     DKRTIRSKEVVKDKLLSLARAFRARLGLSEDEKLRQRFVSAGLKGGSQLDLYFTARFV
                     GPAAGAIGGSFIHSNTAFWCLALAAVGYLLPDIWLTRAVKKRQTKIQKGIPDAIDLLV
                     ICVEAGLGMDQALLRIGQEIAVSYPELNEEFNQVNLEQRAGKPRLDAWQSLAERTKLE
                     EFQSLVTMLQQTDRFGTPIVRALSRYSEEIRTARRQRAEEAAAKTKIKILFPLVFFIF
                     PCLFIVLLAPAILTLTSTLK"
     gene            1563..1913
                     /locus_tag="VHE33_12560"
     CDS             1563..1913
                     /locus_tag="VHE33_12560"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HVT98330.1"
                     /translation="MHYLTVTIPKKAVTIGARIGLADTFLSRLVGLLGKSSLDDGAGL
                     LIRPSSGVHTMWMRFAIDVIALDRDLRVLKTWQRLPPWRMTEVSFKTHSVLELAPGQI
                     HHLNIEPGDQLALG"
     gene            1979..2434
                     /locus_tag="VHE33_12565"
     CDS             1979..2434
                     /locus_tag="VHE33_12565"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVT98331.1"
                     /translation="MVPGHRTFGSAVPCPEEAVFVELMRAADRLARRPAQFLKKHNLS
                     SNQYNVLRILRGAPDGLLCGEIACRMISRDPDITRLLDRLEKRGLIGRCREDPDRRKV
                     LVRITSAGLTLLARLDKAVCDLHRSQLGHLAPRQLRQLSLLLVAMQQTG"
     gene            2529..3089
                     /locus_tag="VHE33_12570"
     CDS             2529..3089
                     /locus_tag="VHE33_12570"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HVT98332.1"
                     /translation="MSATAVVPAATTTWTIDPVHSTAEFRVKHMMIANVRGRFTGVTG
                     KLAYNAGDVAKSSIEASVDVATIDTRDPQRDTHLKGSDFFDVEKFPTMTFKSSKVTRK
                     SNGSLSVSGPLTIHGITKDVEFEIEGPTPAVKDPWGNTRIGVIATTKIDRREYGLNFN
                     AALETGGVLVGEEVHITLELEFVQAA"
     gene            3224..5509
                     /gene="hypF"
                     /locus_tag="VHE33_12575"
     CDS             3224..5509
                     /gene="hypF"
                     /locus_tag="VHE33_12575"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HVT98333.1"
                     /translation="MRGVVQGVGFRPYVYNLARRHALGGFVLNSARGVFIEIEGDPVT
                     IESFVASLSAEAPPLVRIAQILRKEIPVTGDTAFVIHESDSAGSAFALVPPDICVCDA
                     CLADTRDPDNRRYDYPFTNCTNCGPRYSIIVDVPYDRPMTTMAEFAMCSDCLREYKDP
                     ENRRFHAQPNACPVCGPHLELAEPKVEANHASSNSQSPRSVLLRVADVLRGGGIVAWK
                     GLGGYQLACDARNAAAVEELRRRKHRNEKPFAIMVATEAVAESLCFLSAEERSALISR
                     ERPIVLLRRRSGVDLADAVSPGNPATGVMLPCTPMHDLLFRILRERWETEPALIMTSG
                     NISEEPIVTENAEAEERLAGIADAFVHHNRRIHTRVDDSVVRIVAGQAMPIRRARGYA
                     PMPVFLGRGEAEVLACGAQQKNTLCLTRAGFALPSQHLGDLENFETLRFFEETLDRMK
                     RLFHVTPAAVAYDLHPGYLSTQFALALPDLQKIGVQHHHAHIASCMAEHQLDGEVIGV
                     AWDGTGFGTDGTIWGGEFLAAGYLGFERYARLRPVLLPGGDSGVREPWRVARSYLMDT
                     FDSIPDHPHWHAMASPERLRVVDQMLVKKINTMPTSSAGRLFDAVASLIGLRQTVSFE
                     GQAAMMLEAAAAGDSSTTAYGFSLTGNSPIDIDLRSMIREILAGIEAGEPQPRLAARF
                     HATLVAVIAEVCRRMRVDLGFNRVCLSGGCFQNELLLEGCLHALRHDGFEVFTHREVP
                     ANDGGISLGQAAIACETLRQQ"
     gene            complement(5582..6076)
                     /locus_tag="VHE33_12580"
     CDS             complement(5582..6076)
                     /locus_tag="VHE33_12580"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVT98334.1"
                     /translation="MSKFREAVYGKGHFHLITCTCYQRLPKLAEEKHRDVFVRLMEEL
                     RVKFRFAVVGYVVMPDHFSLLMREPEIDTAANAVEMLRARYRRRYNSSARSDEQVWES
                     RWSDTHIVGAEAVASSLASMHAEPVKARLAESPEEWKWSSARAYAGLPEGVVTVEQVP
                     AASV"
     gene            6172..>6419
                     /locus_tag="VHE33_12585"
     CDS             6172..>6419
                     /locus_tag="VHE33_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98335.1"
                     /translation="MRFARYPSAVFLLLLFTACSRDRLILPNSSVLLPADGRTHTVTT
                     IVRQSGSVLTPAQIESASPELHFAPHPGNSLAVLVRAPV"
BASE COUNT         1166 a         2181 c         1781 g         1291 t
ORIGIN      
        1 tgcttccggt ggtggtgatg ggcctgatca acgtcatgga tcccggctac tcgaaaccac
       61 tgctcaagga tccgttcgga cacaagctcc tgctgctctg tgtctgcctc atcatgctgg
      121 gggctttttt catcaaccgc attatcaatt cgatcgacgt ttagggagag agactcgccg
      181 ccatgatgat cttcttctac attgcgctct ccttcacggt cttcttcgtg atcacgctgc
      241 tctgcgcgcc cttcctgctc cgcccctctg tgcaggcccg ccgcatcctg gacgtggtga
      301 cgagcgatcg cccggacaaa cggacaatcc gcagcaagga agtcgtcaag gacaaactgc
      361 tctctctggc gcgcgccttt cgcgcccgtc tggggctctc cgaagacgag aagctgcgtc
      421 agcgcttcgt gagcgccggc ctgaagggtg ggagccagct ggacctctac ttcaccgccc
      481 gcttcgtcgg tccggcggcg ggcgccattg gcggcagctt catccacagc aataccgcct
      541 tctggtgtct tgccctggcc gctgtcggat atctgcttcc cgacatctgg ctcacgcggg
      601 ccgtcaaaaa gaggcagacg aagattcaga aaggcattcc cgacgccatc gacctgctcg
      661 tcatctgcgt tgaagccggt ctgggcatgg atcaggctct tctccgcatc ggccaggaaa
      721 ttgccgtcag ttatccggag ctgaacgaag agttcaacca ggtcaacctt gaacagcgcg
      781 ccggcaagcc ccgtctcgac gcatggcaga gtctggccga gcgcaccaag ctggaggagt
      841 ttcagtccct ggtcacaatg ctccagcaga ccgatcgttt cggcactccg atcgttcgcg
      901 ccctgagccg ttactcggaa gagatccgta ccgcgcggcg gcagcgcgcg gaggaagcgg
      961 cggccaagac gaagatcaag attttgttcc ctcttgtctt ctttattttt ccctgcctgt
     1021 tcatcgtgct gctggctccg gccatcctca cgctgaccag tactttgaaa tgatccgcag
     1081 gttcgtcggt ttgcgaaaat gtgtgtccgc ctgatcgttg ggtagtgcac cggaaaggag
     1141 atctcaacat ggagtcaagc atgatcgttc gcgttgtggc cctggccatg gcggtgatcg
     1201 ttgtcggcgt catcatcctg cgcagaagga agcagaagtc tgcgtaatat acctgtcgct
     1261 gtagtccggt cctgaatgac ggcgcttgcc gggaaaggag aaaggccatg ggtggatccc
     1321 tgatgattcg tgcagtcgcc ggagcactgg cggtgcttgt tttcggcgtg atcgtcctgc
     1381 gcaggaagcg gacggcctga ttttcaggcc cgtaaccctg gcgatcctga ccctgttcgt
     1441 caggaccgga accggcaatt ctgccgagcg gattctcccg ggcgaagctt tcagttcgct
     1501 tcgggaaatc tctcgtctgc aacgctgtag accgccctgc aaccggcgga agaggcggcc
     1561 acgtgcacta tctcactgtc accattccga agaaggccgt taccatcggc gcgcgcatcg
     1621 gcctcgccga cacgttcctg tcccggctgg tcggccttct cggaaaatcc tctctcgatg
     1681 acggtgccgg cctgctcatc cggccctcct ccggcgtcca taccatgtgg atgcgcttcg
     1741 ccatcgacgt cattgcgctg gaccgggatc tgcgcgttct caagacctgg caacgcctgc
     1801 cgccctggcg catgaccgaa gtgagcttta aaacccacag cgttctcgag ctcgctcccg
     1861 gccagattca ccacctgaac atcgagcccg gcgatcagct cgccctgggt tgagctgtcc
     1921 ccgcatccgc gttcgcagct ttgacactta ttcgttctaa cgaacatcct taatacatat
     1981 ggttcctgga caccgtacgt tcggcagtgc cgttccctgc ccggaggaag ccgtcttcgt
     2041 cgaactcatg cgagccgccg accgtctggc gcgccgccct gcccaattcc tgaaaaaaca
     2101 caacctttcg tcgaatcagt acaacgtgct ccgcatcctc cgcggcgcgc cggacgggct
     2161 tctttgcggg gagatcgcct gccgcatgat ctcccgcgat cccgacatca cccgtctcct
     2221 cgaccgcctc gagaaacgag gcctcatcgg ccgttgccgc gaagatcccg atcgccgcaa
     2281 ggtccttgtc cgcattacat ccgccgggct cactctgctg gcgcgcctcg ataaagccgt
     2341 gtgcgatctc caccgaagtc aactcggcca cctcgccccc cgtcagcttc gccagctctc
     2401 gcttctgctc gtcgccatgc agcagaccgg ctgattcggc tgccggtcct ggccgcacgc
     2461 gtttttacag atatttgttg caacgaacat ctccttgccg catccattga tgaggaggag
     2521 tttccgccat gagcgcaacc gctgttgtcc ccgctgccac caccacctgg accatcgacc
     2581 cggtccactc caccgccgaa ttccgcgtca agcacatgat gatcgccaat gtccgcggcc
     2641 gcttcaccgg tgtcaccggc aagcttgcct acaacgccgg cgacgtcgcc aaatccagca
     2701 tcgaggcctc cgtcgacgtt gccaccatcg atactcgcga tccccagcgg gatacccatc
     2761 tcaaaggttc cgacttcttc gatgtcgaga aatttcccac catgaccttc aagtcatcga
     2821 aagttacccg taaatcgaac ggctccctct cggtctccgg cccactcacc attcacggca
     2881 tcacaaagga tgtcgagttc gaaatcgaag gtccgacgcc ggctgtcaaa gacccgtggg
     2941 gcaacacccg cattggtgtt atcgcaacca cgaagattga ccgtcgtgag tacggactca
     3001 atttcaacgc agcgctcgaa accggcggcg tcctggttgg cgaagaagtg cacatcaccc
     3061 tcgaacttga attcgttcag gccgcctgag cggcggcgcg gaacatcggc ccaccctgcc
     3121 cagggggaat cgcctgtgaa aggatgaact gtccgggttt cggaatgcac ccaggagaga
     3181 aacggcgatt cccgacggcc tgacaatccg cctgcaactg cagatccgcg gcgtcgtcca
     3241 gggcgtcggc ttcaggccct acgtctataa cctcgcccgg cgtcacgcac tcggtggatt
     3301 cgtcctcaat tccgctcgtg gcgtgttcat cgaaatcgaa ggcgatcccg tcacgatcga
     3361 atccttcgtc gcttcccttt ccgccgaagc gcctccgctg gtccgcatcg cccagatcct
     3421 ccgcaaagaa attcccgtca ccggcgacac cgccttcgtc attcacgaga gcgattccgc
     3481 tggttcggcc ttcgccctgg tcccgcccga catctgcgtg tgtgatgcct gcctcgccga
     3541 cacacgcgac cccgacaatc gccgctacga ctacccgttc acgaactgca ccaattgcgg
     3601 tccgcgctac tcgatcatcg tggacgttcc ctacgatcgc cccatgacca ccatggcgga
     3661 attcgccatg tgctcggatt gcctgcgcga gtacaaggac ccggagaacc gccgattcca
     3721 cgcccagccg aacgcgtgcc ctgtctgcgg cccgcatctt gaacttgccg aaccaaaggt
     3781 ggaagcgaat catgcatcgt ccaattcgca gagccctcgt tcagtcctgc tccgggtagc
     3841 cgatgttctc cgcggcggcg gtatcgtcgc ctggaaggga cttggcggct atcaactggc
     3901 ctgcgacgcg cgcaacgctg ccgccgttga ggaactgcgc cgccgcaaac accgcaacga
     3961 gaagcccttc gcgatcatgg tggcgaccga ggccgtcgcc gagtcgctct gcttcctctc
     4021 ggctgaagaa cgcagcgccc tgatctcgcg cgagcggccc attgtgctgc ttcgccgacg
     4081 ttcgggcgtc gatctcgctg acgccgtctc gcccggcaat ccagcaaccg gcgtgatgct
     4141 tccctgcacg ccaatgcacg atcttctctt ccgcatcctg cgcgagcgct gggagactga
     4201 accggcgctc atcatgacca gcggcaatat cagcgaagag ccgatcgtca ccgagaacgc
     4261 ggaagccgag gaacgcctcg ccggcatcgc ggacgccttt gttcaccaca accggcgcat
     4321 ccacacgcgc gtcgacgact cggtggtccg catcgttgca ggacaagcga tgcccattcg
     4381 ccgcgcccgc ggctacgccc ccatgcctgt gttcctcggc cgcggcgaag ccgaagtcct
     4441 ggcctgcggc gcccagcaaa agaacactct ttgcctcact cgcgccggat tcgccctccc
     4501 cagccagcac ctgggcgatc ttgaaaattt cgaaacgctg cgcttcttcg aggagacgct
     4561 cgatcgaatg aagcggttgt tccatgtgac gccggcggcc gtggcctacg atctgcaccc
     4621 cggctacctg agcacgcagt tcgcgctcgc cctgccggat ctgcagaaaa tcggcgtaca
     4681 gcaccaccac gcccacattg ccagctgcat ggccgagcac caactggatg gcgaggtcat
     4741 cggcgtggcc tgggacggca ccggcttcgg caccgacggc acgatatggg gcggtgaatt
     4801 cctggcggca ggctatttgg gatttgaacg ctatgcgcgg ctgcggccgg tgctgctccc
     4861 tggcggcgac tcgggtgtgc gcgagccctg gcgcgttgcc cgcagctatt tgatggatac
     4921 gttcgactca attccggacc atccgcactg gcatgcaatg gcctcgcccg agcgcctgcg
     4981 ggtcgtcgac cagatgctcg tgaagaagat caacacgatg cctacctcct ccgccggacg
     5041 cctcttcgac gccgtcgctt ctctcatcgg cctgcgccag acggtttcgt ttgagggcca
     5101 ggcggcaatg atgctcgaag cggccgctgc aggagactct tcaaccactg cttacggttt
     5161 ctctcttacc ggcaactcgc ccatagacat cgaccttcgc tcgatgatcc gtgagattct
     5221 cgccggaatc gaggccggag aaccgcagcc gcgcctcgcc gctcgcttcc acgcaacgct
     5281 tgttgctgtg attgccgaag tctgccgccg catgcgcgtc gatctcgggt tcaaccgcgt
     5341 ttgcctcagt ggcggatgct ttcagaacga gctgctcctc gaaggctgcc tccacgctct
     5401 gcgccacgac ggattcgagg tcttcaccca ccgcgaagtc cccgccaacg acggcggcat
     5461 ctccctcggc caggcagcga ttgcgtgcga aacgctccgg cagcaatgat ggaggcagag
     5521 agacggcggg acaggcccag tcggcgtggg ccagggcgca gcctcttacc gacgcgtgca
     5581 gtcagacaga cgcggcagga acctgttcga ccgtcacgac tccttcggga agaccggcgt
     5641 acgctcgggc gctgctccat ttccattcct cgggactctc cgccagcctt gccttcaccg
     5701 gctccgcgtg catcgaggcc agggaactcg cgacagcctc ggctcccaca atatgcgtgt
     5761 ccgaccagcg gctctcccaa acctgctcgt ccgatcgcgc ggagctgttg tacctgcggc
     5821 gatatctggc ccgcagcatt tcgaccgcat tggctgcggt atcgatctcc ggctcacgca
     5881 tcagcaggct gaaatggtcc ggcatcacga catacccaac aaccgcgaag cgaaacttga
     5941 cgcgcagctc ttccatcagg cgtacgaaca catcccgatg cttctcttcc gcgagcttcg
     6001 gcagtctttg gtagcaagtg cacgtgatca gatggaaatg gcccttcccg tagacggctt
     6061 cacggaactt tgacataacg ttgagtgtaa gagcagcaac gcgattcttt tgaatttcgt
     6121 ggttacgagg cctcgacaga gagtcaaaaa cgaatgagca ggcttccaac agtgcgtttc
     6181 gcgcgatacc cgtccgcggt cttcctgctc ctcctcttta ccgcctgctc ccgtgatcgg
     6241 ctcatcctcc ccaacagctc cgtgcttctg cctgctgacg ggcgcacgca caccgtgacc
     6301 acgattgtgc gtcaaagcgg aagcgttctg acgcccgcgc aaatcgaatc ggcctcgccc
     6361 gagctgcatt tcgcgcctca tcccggcaat tcgctcgcgg tgctcgtgcg ggcgccggt
//
LOCUS       DASZYN010000489         1662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_401242, whole genome shotgun sequence.
ACCESSION   DASZYN010000489 DASZYN010000000
VERSION     DASZYN010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401242"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VHE33_12590"
     CDS             <1..585
                     /locus_tag="VHE33_12590"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HVT98336.1"
                     /translation="ILFGEVNPSGRLPITFAKTDAQLPHPEVPGMDLIKNPGPGHHRP
                     GELPLFDAPATEGVRVGYKWFESKQEEPLFPFGFGLSYTTYAFSDLKASEHEVTVTVR
                     NTGNRAGTEVAQVYAVLPKSTGESDFKRLVAWDRVALAAGESKTVTLKIDPLYLSIFD
                     VSKNQFVLAPGDYEILAGSSSHDTAVNTTVHVAD"
     gene            complement(664..1077)
                     /locus_tag="VHE33_12595"
     CDS             complement(664..1077)
                     /locus_tag="VHE33_12595"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HVT98337.1"
                     /translation="MPGEAAAVIGKSVQIRGEVKGNEDLMVEGLVEGTVTLTEHRLTI
                     GTNARVTANVTARDVVVQGSLQGDIHAQGRVELRAGSRVNGDIRAARLSVEDNAIYSG
                     KVELDGSTAEGGKGAAAHPPATTASTQPASPGSAN"
     gene            complement(1158..>1662)
                     /locus_tag="VHE33_12600"
     CDS             complement(1158..>1662)
                     /locus_tag="VHE33_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98338.1"
                     /translation="IALTITTSDAALARILEPRAPRPDLRFRTVERLRAAGLKAGILC
                     SPLMPGITDTPRALEAMARRAKKAGASFFSSQPLFLKPSSKPVFLRFVQEHFPELVKA
                     YEARYGERAFVSKAYQERVSALVRAVVRKHRLEQRFGDEPLPGKKPAWPVQAELWPGA
                     AKLQRTA"
BASE COUNT          301 a          530 c          509 g          322 t
ORIGIN      
        1 atcctctttg gggaagtgaa tccatccggg cgtctgccga tcacgtttgc gaaaactgac
       61 gcgcagctgc cgcaccctga ggtcccaggc atggacctga tcaagaatcc ggggccgggt
      121 catcacagac cgggcgaact gcccttgttc gacgctccgg caacagaagg tgtccgtgtc
      181 ggttacaaat ggttcgagtc caaacaggag gagccccttt tcccgttcgg attcggactc
      241 tcgtacacca cgtatgcgtt ctccgacctg aaggcctcag aacacgaggt tacggtcacg
      301 gtgcgcaaca ccgggaatcg cgccggaacc gaggtcgcgc aggtctatgc ggtcctgccg
      361 aagtcaaccg gcgaatcgga tttcaagcgc ctggttgcgt gggatcgcgt tgcgctggca
      421 gcgggcgaat cgaagacggt cacgctgaag atcgacccgc tctatctgtc gatcttcgat
      481 gtcagcaaga accagttcgt gctggcgcct ggagattatg aaattctcgc cggctcgtcc
      541 tcgcacgaca cagctgtcaa caccacggtc cacgttgcgg actagctctg cctggcgctc
      601 aaaacaaaac ggccccgcga tcgactcgat ccggggccgt gacatttctg aaatgggaac
      661 gctctagttg gccgatcctg ggcttgctgg ctgcgtgctc gccgtggtgg ccggcgggtg
      721 cgctgcggcc cctttgccac cttcggctgt cgaaccatcc agctcgacct tgccggaata
      781 gatggcgttg tcttccaccg agagccgggc ggcgcggata tcgccattga cccggcttcc
      841 cgcgcgcagt tcgacgcggc cctgcgcatg aatatcgccc tggagcgagc cctgcacgac
      901 cacatcgcgc gctgttacgt tggcggtgac gcgcgcgttg gtcccaatcg tcagccgatg
      961 ttcggtcagc gtgaccgtgc cctccaccag accctcgacc atcaggtctt cattgccttt
     1021 cacttctccg cgaatctgga ccgatttccc gataacggca gctgcttctc ccggcattgc
     1081 gcaaactccc ctcttgtgtt ggctttctcc gcactatacg caacgctacg tcaggccgca
     1141 agaaaattgc cagcggacta tgcggttcgc tgcagttttg cggcgccggg ccacaactcc
     1201 gcctggaccg gccatgcggg cttttttccc ggcaacggct catcgccgaa gcgctgctca
     1261 aggcggtgct ttcgcaccac ggcgcgcacc agagccgaaa cgcgttcctg atacgccttt
     1321 gagacgaacg ctcgttcgcc gtagcgcgcc tcgtaggcct tcacgagttc cgggaagtgc
     1381 tcctggacga aacggaggaa gacgggtttg gaactcggct tcaggaacaa aggctgcgat
     1441 gaaaagaagc ttgcgccggc cttcttcgcg cgacgcgcca tggcttcgag cgcccggggg
     1501 gtgtcggtaa tgccgggcat gaggggtgaa cacaggatgc cggccttcag cccggcagca
     1561 cgcaaacgct ccacggtgcg gaagcggaga tcgggccgcg gggcgcgcgg ctccaggatc
     1621 cgcgccaggg ccgcatcgga ggtcgtaatt gtcagggcga tc
//
LOCUS       DASZYN010000490         1889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1047605, whole genome shotgun sequence.
ACCESSION   DASZYN010000490 DASZYN010000000
VERSION     DASZYN010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047605"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1889)
                     /locus_tag="VHE33_12605"
     CDS             complement(<1..>1889)
                     /locus_tag="VHE33_12605"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVT98339.1"
                     /translation="VHLARAQVTFYQAEVPLLFLQDSTDGIFVHMRGQPPASLRCGDI
                     VAVDGFTGSGKLTPVIVDARFRLLGHAPLPKPAPTSFDRVLAGAVDSQWIAVRGIIRS
                     IRPVWDGDEVAITLASGPDRLEVMTPDFVARNPGGLVDARVVIRAAAGARFNDRKQLI
                     GTYLFMPDMSYLEVEVPAIDTFTRPPRRVEDVMRPPQRGPGHRVHVQGVVTSTWGNQH
                     FSLMAREHGIFVATEEPVTVHVGDVVDAAGFPAIGDYTAVLENSILRQTGTAPPPAAV
                     HLTAAEALAGDHDAESIEVDALYLDHTREDTGSPDMVLSADGISFLAVLPPGSRSEDL
                     ERFKLGSRLRVRGICVIHVADDKTPRELSILLRSPEDIVVLHRPSWWTTGHTLLIVLP
                     LGAVILIVVVWNLVLRQQVRRHTDTIRQQLREADALRVQAEAAHQEKSDSLASVLLLQ
                     HDLLEAQEKLRHQATHDALTGLWNRRALLELLEKELERCGRTGSSIGILMADVDHFKP
                     VNDTLGHLAGDQVLKEIALRITHATRGYDLCGRYGGEEFLILLPGCNGEETQAGAERI
                     RMAIASAPFRAAGSTVNLTISIGATLAPQCARTETELLSLADQALYEAKNTGRNRTVV
                     CTAAEV"
BASE COUNT          367 a          635 c          562 g          325 t
ORIGIN      
        1 acctccgccg ccgtgcacac cacggttcgg tttcgccccg tattcttggc ctcgtaaagc
       61 gcctggtccg cgagactcag aagttccgtc tctgtgcggg cgcactgtgg agcaagagtg
      121 gccccgatgc tgatggtcag gttcaccgtc gagcctgcgg cccggaaggg tgcagaggcg
      181 atcgccatgc gaatgcgctc ggcgccggcc tgagtttcct caccgttgca ccctggcagc
      241 aggatcagga attcctcgcc gccgtagcgt ccgcacaggt cgtagccgcg agtcgcatgc
      301 gtgatgcgga gggcaatctc tttcaggacc tgatcgcccg ccagatggcc cagagtatcg
      361 ttcaccggct tgaagtgatc gacatcggcc atcaaaatcc cgatcgaaga cccggtgcgc
      421 ccgcagcgct ccaattcctt ttccagaagt tccagaagtg cccggcgatt ccacagcccg
      481 gtcagtgcgt cgtgcgtcgc ctgatggcgc agcttctcct gggcttcgag cagatcgtgc
      541 tgcagcagca gcacgctggc cagcgaatcg cttttttcct gatgcgcggc ctcagcctgc
      601 acgcggagcg cgtcggcctc acgcaattgc tggcggatcg tatccgtatg acgccgcacc
      661 tgctggcgta aaaccagatt ccagacaaca acaatgagaa tcacagctcc cagaggcagc
      721 acaatcagca gagtgtgccc cgtcgtccac cacgacggcc ggtgaagcac aacgatgtcc
      781 tccggcgacc gcagaagaat actcagttcc ctgggcgtct tatcgtcggc gacatgaatc
      841 acgcagattc cccgaacgcg cagccggctg cccagcttga atcgctccag atcctcactg
      901 cggctgcccg gagggagtac ggccaggaaa gaaataccgt cggcgctgag caccatatcg
      961 gggctgccgg tgtcttcgcg cgtgtggtcc aggtagagtg cgtccacttc gatcgactcc
     1021 gcatcatgat cgccggcgag cgcttccgct gcagtcagat gaaccgccgc cggcggcgga
     1081 gccgtgcccg tctggcgaag aatcgagttc tccagcaccg ccgtgtagtc cccgatggcc
     1141 ggaaatcccg ctgcgtcgac aacgtcgccc acatgcaccg tcaccggctc ctccgtggcg
     1201 acgaagatcc catgctcacg agccatcagg gaaaagtgct ggttgcccca tgtggacgtg
     1261 accacaccct gcacgtgaac tctgtgtccg gggccccgct ggggcggcct catcacatcc
     1321 tccacgcgcc gaggcgggcg cgtgaaagtg tcgatggccg gtacttccac ctccaggtag
     1381 gacatgtccg gcataaaaag atacgtgccg atgagctgct ttctgtcgtt gaaacgcgcc
     1441 cccgcagcgg cgcgaatcac aacccgggcg tcgaccaggc cccccggatt gcgcgccacg
     1501 aaatccggag tcatcacctc gagccgatcc ggccccgatg ccagcgtgat cgcgacttcg
     1561 tccccgtccc agacagggcg tatcgaacgg ataatccccc gaaccgcgat ccattgagag
     1621 tccaccgcgc cggccagaac ccgatcgaag ctcgtcggag ccggctttgg cagaggcgcg
     1681 tgacccagaa gccggaagcg tgcatcgaca atcaccgggg tcagtttccc ggaaccggtg
     1741 aacccgtcca ccgcgacgat gtccccgcat cgcagcgaag cgggaggctg ccctcgcatg
     1801 tggacaaaga ttccgtccgt cgagtcctgc aggaagagaa gtggcacttc cgcctgatag
     1861 aaagtcacct gagcgcgagc cagatggac
//
LOCUS       DASZYN010000491        17887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_728596, whole genome shotgun sequence.
ACCESSION   DASZYN010000491 DASZYN010000000
VERSION     DASZYN010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 17887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17887
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728596"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..788
                     /locus_tag="VHE33_12610"
     CDS             147..788
                     /locus_tag="VHE33_12610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT98340.1"
                     /translation="MNRPDKRVTRILIVDDHPVIQAGLASMLATHPEVRVMGAVSSGE
                     EALARMEEDMPDIVLLDLRMPGMTGIDLLHAMRQKKMCARAIVLTSFETDENIYRAIQ
                     VGAQGYLLKDTSQREMLQAIATVHAGRKYIPGQIAARLAERMMRSSLTARELEILNML
                     AKGLTNKQIGGVLKISDNTVRNHVNSILEKLEVADRTEAVTVAIQQGIIQIDS"
     gene            990..3089
                     /locus_tag="VHE33_12615"
     CDS             990..3089
                     /locus_tag="VHE33_12615"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HVT98341.1"
                     /translation="MLLCILLTGLGAVAQDTAYPPQDAQIPGPSTKADFPAWLADIQH
                     WRAERLMRMGYYETNYARPELKWTQRDFVCVQMMIEERTFFDANNSVYTVDAFLDRLK
                     NEFGGVDSVLIWDTYPNLGVDNRNQFDRLRDMPGGIEGVRQVVAAFHRRGVRVFFPET
                     PWDMGTRDEGVPDWTAQAQLMKDVGADGLMGDTLDGMPDAYLQASNAVRHPLALHPEG
                     IPPDETLAWNEMTWGYWSYPFVPMISRYKWLERRHMPMLVSGGRHHIPEIQAGFFNGV
                     GVSDQEDVVGIHNEFTAREDEALRRTTRIKRTFGDAMVSAKWEPHTPTVQYGVFASRF
                     PGKAETVWTLINHNPYEVEGRQIEVPYQPEIRYYDLWHGEELKPEIHGAIATLSFRME
                     PEGFGAVLAIESASLSQPAQDLLAEMHASATKPLTAYSDDWQPLQQQMIPIARAKANA
                     EARKDMVRIPAGTFEFRVHGIEVNVKDDAGLDVQESWEDAPRRFHDYPMTVPTFYIDR
                     YPVTNEQYKAFLDATHYHPRDDHNFLRDWSNGTYPAGWGECPVTWVSLEDARAYARWA
                     GKRLPHEWEWQYAAQGADERIYPWGNQWDPSAVPPPDKGRALTGPAEVTAHPRGASPF
                     GVMDMVGNVWQWTDEFEDTHTRSAILRGGSYYQPQGSMWYFPQAYRLDEHGKYLLMAP
                     SLDRSGTIGFRCAADAD"
     gene            3136..5724
                     /locus_tag="VHE33_12620"
     CDS             3136..5724
                     /locus_tag="VHE33_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98342.1"
                     /translation="MDSVDLKSRRRFLKGVLALPAAGTLSRFSLWPSTNDRSGRLPAQ
                     TPQAEPGRIENAYVKFLPGERESLEQTPRLIAVHSDAVVADFNGTQRLVAVGASNEGW
                     LLAAIIPWLNGTPTAVLEKHVTHRGVLVYLTTDGEIARIPKFVGDLSQIRPRPIQPPS
                     GVKFERPARYVPGPDVLGNAVLQSDRDPEYENVAALGEEFIGWTLVANEECGPERSLW
                     LEADGRSREFGTDAQSLWAPDMTGRYFDPQRLLPSGYLYDYVHGYSKRTMLGGFLPAA
                     DIGVWNPEYETGYEVMMVLPPGGDARPIARVRAMVPEADGDRLIPDGDSGAPQKSQAG
                     ESVDRYWNGSAADFFTAAVSAWNRWHHFFDDRMQVEIPDEWLLQAARGGIALSRSSYR
                     GLQPTYQIGEGAYTKIPERSHALFPVAHYEFVWAHQLWNLTAEVEPCFQFYLDHYILP
                     NGNFLYNTQDQVEAPLNAGVFLENSARAWDYARDLDALRARMPILRRMAEHVRGRIAY
                     SKSHFPASDPRHGLVWGSPEADLGDPRDDYPESHPFYYQNAAWTWRGFREHARALASA
                     AAAHSDPALLEESRIYAAWATDLRADVESSLSKVLALRNPKMKQAGITPFTPFDTHRK
                     PTDLSSYENHRFMEDWWMSDWGDPELDLGHFRHREIAGLQILGMNTDGAYPRTSNFMS
                     HGTLAGRIRQEDYRPFLLALYALCCYTMDSGSRYSPEDAMLPGGYPGDGSPYGWSAVV
                     NSELQAALGLRWLLCYEEHDQDVVHLQKAAPKSWFLPGERIQVTNCPTRFGTVSWTTT
                     TRNGGGWKMNLALPQPFAADLHVHIHPPDGRPLRSASLGTVEKACVVLPAGLIANRTN
                     LEIEIS"
     gene            complement(5979..6896)
                     /locus_tag="VHE33_12625"
     CDS             complement(5979..6896)
                     /locus_tag="VHE33_12625"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HVT98343.1"
                     /translation="MALRSPEANRLFDSAQRLSQKTIKSMHGALRSTPNQAIVWGMLE
                     RNPAVGVKLPRKKSRKPPVLLALPEIRAMLEELPEPTKSIVTLIVFGSMRVGEVLALR
                     WKRILKDRIQVAERVYDGEFDDVKTEAGEREVPFDNLGAIAGALRRTWESSKFHGLDN
                     LVFANKAGNLLDKHNLLQRHIKPAAMKLGLSRTIDFRSFRTMHASLMRRTGARPEVTR
                     DNMGHADCDVTLNVYSESWWDEGVAAVSRAVGAVFAEPAAKPVARPLEEQKGEDSVIE
                     WVPFWVPQPVSDPQAQAYIVDLNGRGERI"
     gene            complement(6868..8361)
                     /locus_tag="VHE33_12630"
     CDS             complement(6868..8361)
                     /locus_tag="VHE33_12630"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT98344.1"
                     /translation="MKSRAFLIWLLPCVVAMGVLTASAQVSTVPQIAQMLRTQQYEQA
                     LSNTNALLQKQPRDCRLLSLRGMALNGLARSSEALQAFRQALQSCPDDLLPLEGAAQI
                     EYARRQPDAANLLTRILAIRPDDVTSHAMLATVDRARRDCQAALPHYKASRSLFGVRP
                     EMAQGYASCLASTGDYSHAAEEYRQLLLTHPDATSRYNLAIVLWKMQEPRQALAALEP
                     LLTPPTDETALVLGSQIAEEAGDTPHAVQLLRLAIVLDPKRTDNYLNFAEIAFNHSSA
                     AVGIDMLNFGIVHLPNAASLYVARGVLEVQISKTENAIADFERAHRLKPQLSLATDAV
                     GIMKSQQHEFAASVELFRQQAQQHPKDSLLWYLYAEALSQSTKGGPRSPEALAAAERS
                     VALDGNYVPARDLLALLYLQASQPQRALEASLAALKIDPDDETALYREIIARRTLGQH
                     DEAQQLVPRLQEIRNKNMKRQQLSHSYVLRDEMAQPEEDGAKKPRGQ"
     gene            complement(8491..11847)
                     /locus_tag="VHE33_12635"
     CDS             complement(8491..11847)
                     /locus_tag="VHE33_12635"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT98345.1"
                     /translation="MQFRKFIVSLVFASFFYVMPAFAQFSASVQGTVLDPRGNAVPGA
                     AVTLANMDTSVMETKTSDPTGSFRFVSLAPGHYLVSASANGFATSKVDLVLSTAENRD
                     VPITLAVGQVASTVSVSAQSPLLDTSDSRNQLTIDSQAVQSLPLAARNPTALIALAPG
                     VTGLGSAGYTNFFTENADYSANGRGNNGNQYVLDGLDINIDVNPGVLSLVPNADALSE
                     MSVQTNTYTVDYGKTSSMQTVMTTKSGTEAYHGFGALYYTYQGLNARGEFGPPPSVRI
                     APFHTTNMSFGVGGPVIPHHQFFFFFSIEPYLALTSNGNAVQTYEDPAFVAFAQQVQP
                     NSPEVQLMAKYPPSGATTTNVAATALDTFGPQDVAGNSGCGTPSTDNIPCSTPVFDNG
                     NFNSSSFNNSKQYNLRIDKYFSKDRLYGTLFRDTTNEGGPSVRPAFNTTNPSSVIATQ
                     GSETHTFSQNTLNQAFFGYNYLQGTASATGQLSVPVVNVNNLGVGWGIGFADGQYAEH
                     NYHWRDVLTKLHGTHAISVGYEGWHGDDLALFAPSYAQPSFQFNNMIDLINNNPYSES
                     SLSYNPVTGKPMPGQYAYAETTHGVFAEDTWRASRRITVNYGLRYDNFGNPYPIRNTP
                     LANFHLGTGSTMAVQVANGVNRQQSHVYDQSMNWIFSPRGGVAWDLTGNGNWVLRGGY
                     GIYRDWVTLGNAENNLKGNPPGFVLPTFYNNGSTAAPIFGYGTSNTYPFGFPYPAFQG
                     APLDAKGGLPGQQIGVGGVDGNLKSPYTETWSAALEHQLARNMVASASYVGQHSGDLQ
                     YGGGSSFGNSFSGDVNVYAGDLLQHLTCTPTGQGQAATCTGTQTRLNTSFGAINYTFN
                     GPWSNYSALILALQGRFAHRGFVDASYTRASSKDNSGALPIGNYPIEYPLSRFYGPSA
                     WDAANRFSLGWSYVLLGVNSGHGFLGRIASGWEVASTTVLQSGLPFTVASSGALDVAK
                     DSSGTVGFRPDSGDFNADGDNNDFPNVGSYHMSHSRRSYITGVFPHCSGTNLDSCGPF
                     TLPSVGEEGNEQINQFRNPGFTETDATLKKTTDITERLKFELRIDYFNLFNRVNLNGV
                     NIDAASGSSFGTSTSTQIPRQGQLGARLEF"
     gene            complement(12021..14453)
                     /locus_tag="VHE33_12640"
     CDS             complement(12021..14453)
                     /locus_tag="VHE33_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98346.1"
                     /translation="MNRRDFISMLAATSAALRSSRALGAPRMHSASSSPHPSAPPYSP
                     TAFPRDDYTPFGYLDNPWHTWDLHPSGVLRSVPGIGLALYYPAGPGGYFDFRRNHVYT
                     AELALGFRIGERILFSPGDFAASQLSSPHHTKNVLVYAFEESGLRVTSSFYQVNEDCI
                     AAQIQFEERAGRTRSVEWLAAHTYVLGGSQWWGGDGVTGGFDAENSLLWTHGFAAGTV
                     FGITARDRSDQHFFSTQEADRGSWLTHEPKTGAGVAYGTDPLYGGMRCQLDIAPHSQT
                     EALVVMQRAPNLGELRRKVRTSLVEAQRELDHRLAEDAAFWSSAPVLAGDWPETWKRG
                     WVYDFETLRTMVRRPIGAYRHPWDGMQIQAPRNVLAETSIDMWALSYANPEMAKEVFA
                     GQFLDALEPNIPCMREDGVMNMVASDGSECGTSISWCFPFFCAASIFDRTRDLAWLRR
                     LYPGLAALLRWTLKHRTDAQGFVVGKCSWETGMDTSRRFQIQQPTGGELVEFLPLVEL
                     QAAASQAGAILSRFAPLAGDDGSVSEWRTIEKTYAGKTQQLWQNDWFHDFDSRTMKLV
                     INADRDPSQAAPAFCGMATDEQKKLLIPTLHKMYESLVAESGDPKSTPDNQLNWSSFV
                     LPFLESAWASGDRQLASALVETIANRIYPSMDRRSVSPGDDSHPRLGWPGVSCEVWGA
                     HGAFGGEVYGWGGVLPAHIVRNLVGLRETDDPRQAILAPGFPSSLAAPGREYALRRIP
                     WGGHLLSVHCRFLDESRVQARISGTGPLAVNEIYDEQQQALVIHRDGTSAMVELLNFG
                     LYRIVFAASSRP"
     gene            14804..15748
                     /locus_tag="VHE33_12645"
     CDS             14804..15748
                     /locus_tag="VHE33_12645"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HVT98347.1"
                     /translation="MRPLAWAAGGLLLALLPGCRSDPPTIAVIPRTTGISLWEPEHAG
                     AEDAAHGDGFRIYWNAPTREDDVQGQIALVERIIDRKYAGLVLAPDQTLALMTPVRRA
                     LSKKIPVVIVGSPLPLQPGGKLTYIVSDDAAAGRIAAERIGSILNGKGTVAILGINPD
                     IAGVLERVRSFEKTLAQQYPEIQVEEKRPGLFSVPYEQQSAEEVLEKHPHLNAILAVT
                     TDATRGTYSALVEAHRAGEVKLVGCDQDLLMPLMTGEMDSVIAEDTYQMGYRAIEVLA
                     DERAGRPVPSLILCAPHLVTKENLETPKIRRVLGLVGR"
     gene            15754..>17887
                     /locus_tag="VHE33_12650"
     CDS             15754..>17887
                     /locus_tag="VHE33_12650"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HVT98348.1"
                     /translation="MAANGKSRRLILVGLAVCFLAGLATTADLLYLHSSTRGLPYHDS
                     FTEGKADEWKAFGGTWAVYDGGMRNDSDERGAKLMTGSGYWTDYVLEADIRLLGEDGD
                     AGLVIRASDAEEGVDSYRGYYAGLRTRDNRLTLGLADHGWQEYEDAPIPHGVQAFQWY
                     HLKLLAWDCDVVASATFPLQNGETTTAAVHDRGCQKAGGIGLRSYSSGGIWRNVRVRR
                     ATAADLQAMLAGHTLREITSNQPYWNGTSIFGSGSLPSHVNPEPALAEAAVQPIASLR
                     MVISSRPVTATVRGVVTLASPALYVQDSTGGVAVLAESSPPLKVGDEVLVTGQVNPHD
                     YSATLDHAGVRLLWTRNPMAPISLTPEQAATGSFTAMFIELEGTFTGATQTPHGAPVL
                     SLAAGQQAFRAVVENSRGSVLAGRLKRGSLLRMRGVCVVNPADTHNLTPFVLLLPSAD
                     DVEVVAGPPWWNTRHVVIIGFFVLLLAFIGHLIYSTVETWRLKAVAEERELLAHEMHD
                     TLAQSFAGLGFQLQAIRNRLPVNMPKVQEQLDLACDLVRHSHEEARRQIMMLRAESPE
                     KAGLAPLLESCARHMLEGGDVQVEAVQEGDCGAIPLRIKDTLFRIGQEALANAVRHAR
                     PSRIRILAHCDDDSVALTIEDDGRGYQHGSEGKGFGVHGMRRRAGSIGANMTIATSPG
                     QGTRVVVLAPLPRRGTLSIWAGLLWKRLR"
BASE COUNT         3577 a         5236 c         5726 g         3348 t
ORIGIN      
        1 gcgggcagca tcggagccaa tatgaccatt gccacatcgc caggccaggg tacgcgggtg
       61 gcggtcctcg ctcccctgcc ccggcgcggc acgctgagca tctgggcggg cctgctgtgg
      121 aaacggctgc gaggccagtc acttctatga acagacccga taagcgagtt acgagaatcc
      181 tgattgttga cgatcacccg gtgattcagg ccggactggc aagcatgctg gccactcacc
      241 ccgaggtgag ggtgatgggc gcggtctcga gcggcgagga ggccctggcc cgaatggaag
      301 aggacatgcc cgacatcgtg ctgctcgacc tccgcatgcc ggggatgacg ggcatcgacc
      361 tcctccatgc gatgcgccag aagaagatgt gcgcgcgtgc catcgtcctc acaagcttcg
      421 aaaccgacga gaacatctat cgcgcgattc aggtgggggc ccagggctat ctcctgaagg
      481 acacctcgca gcgggagatg ctgcaggcga ttgcgaccgt gcatgccggg aggaaatata
      541 tccccggaca aattgcggcg cggctcgcgg agcggatgat gcgatccagc ctgacggcaa
      601 gagagctcga aattctgaat atgcttgcca aggggcttac caataagcag atcggaggag
      661 tgctgaagat cagtgacaac acggttcgta atcacgtgaa cagcattctc gagaagctgg
      721 aagtagcgga ccgcacggaa gcagtcactg tagctattca gcagggaatt atccagatcg
      781 acagctgacc cgcaacccgc attgcgcctg tctgtgtggg atcctgcttc gagatagaac
      841 atctggacta tgtggcgccc tgcaggagtg agtagcatca gctatcagga acggagacag
      901 cctggcgcgt gaacctgccc ctgcctgaag ttttggatga atcctaccac cggtccaggc
      961 gtgcgggaat cacgcgttct ggatggagcg tgcttctctg catactgctg accggcttgg
     1021 gcgcagtggc gcaggacacc gcgtatcccc cgcaggatgc gcagatccct ggtccttcca
     1081 ccaaggcgga ttttcccgca tggctggccg atatccagca ctggcgcgcc gagcgcctga
     1141 tgcgcatggg ttactacgag acgaactatg cacgcccgga actgaagtgg acacagcgag
     1201 acttcgtttg cgtgcagatg atgatcgagg agcgcacgtt cttcgacgcg aataacagtg
     1261 tctacacggt ggacgctttt ctcgaccggc tgaagaacga gttcgggggc gtcgacagcg
     1321 tacttatctg ggatacctac cccaatctcg gagtcgataa ccgcaaccag ttcgatcggc
     1381 tgcgcgatat gcccggaggc atcgaaggcg tccgccaggt agtggctgcc tttcatcggc
     1441 gcggtgtgcg ggtgttcttc ccggagactc cgtgggacat gggcacgcgc gacgagggcg
     1501 ttccggactg gacggcgcag gctcagctga tgaaggacgt cggcgccgat gggctgatgg
     1561 gcgacactct ggacggcatg ccggacgcct acctgcaggc ttccaacgca gtcaggcatc
     1621 ccctcgccct gcatccggag ggcattccac ccgatgaaac cctggcctgg aacgaaatga
     1681 cgtggggata ctggagttac cccttcgttc ccatgatcag ccgttacaaa tggctggagc
     1741 gacgtcacat gcccatgctg gtcagcggcg gccgacatca catcccggaa attcaggcag
     1801 gcttctttaa cggcgttggc gtgtccgatc aggaagatgt agttggcatc cacaacgagt
     1861 tcaccgcacg cgaagacgaa gcgctgcgac gcaccacgcg gatcaaacgc accttcggag
     1921 acgctatggt gagcgcgaaa tgggaaccgc acacgcccac ggtgcaatat ggcgtcttcg
     1981 ccagccgctt tcctgggaag gcggagacgg tttggaccct catcaatcac aacccctacg
     2041 aggtggaggg ccgccagatc gaggtgccgt accagcccga aatccgctac tacgacctgt
     2101 ggcatggcga agaactcaaa cctgagatcc atggcgccat cgctacactc agctttcgca
     2161 tggagccgga gggattcggc gcggtcctgg cgatcgagtc tgcgagcctg tcgcagccgg
     2221 cccaggactt actggcggag atgcatgcgt cggcaacaaa acctctgacc gcctactccg
     2281 atgactggca gccgctccag cagcagatga ttcccatcgc gcgcgcgaaa gccaatgccg
     2341 aagcccgcaa ggacatggtg cggattccgg ctggcacctt cgagtttcgc gtccacggaa
     2401 tcgaagtgaa cgtgaaggac gacgcgggtc tggatgtgca ggaatcctgg gaggacgcgc
     2461 cgcggcgatt ccacgattac ccgatgacgg tgccaacgtt ctacatcgat cgctacccgg
     2521 tgaccaacga gcagtacaag gcttttctcg acgccaccca ctatcatccc cgcgacgacc
     2581 acaactttct ccgcgactgg agcaacggca cctacccggc aggctgggga gagtgtccgg
     2641 taacgtgggt ctctcttgag gatgcgcgcg cctacgcccg gtgggcgggc aaacgtctgc
     2701 cgcacgagtg ggaatggcag tacgccgcgc agggtgctga cgaacgcatc tacccgtggg
     2761 gcaaccagtg ggatccctca gccgttccgc ctcccgacaa ggggcgcgcg ctgaccggcc
     2821 ctgccgaggt gaccgcccat ccgcggggag ccagtccctt cggcgtcatg gacatggtgg
     2881 ggaacgtgtg gcagtggacc gatgagtttg aggacacgca tacccggtcc gcgattctgc
     2941 gtggcggcag ctactaccag ccgcaaggtt cgatgtggta tttcccgcag gcttaccggc
     3001 ttgatgagca tggcaaatat ctgctgatgg cgccgagtct cgaccgctcc ggaaccatcg
     3061 gattccgctg tgcggcggac gcagactgac aaaagatacc ggtgcaatgc gacgcttcgc
     3121 tccggagtgc agaggatgga ttcggtggat ctgaagtcga gacggagatt tctgaagggc
     3181 gtcctggcgc tgccggcggc aggaacgctg tcgcgtttca gcctgtggcc ttcaacgaac
     3241 gacaggtccg ggcggctgcc cgcgcagacc ccgcaggcgg agccggggcg gatcgaaaat
     3301 gcttatgtga agttcctgcc cggcgaacgt gaatcgctgg aacagactcc gcgcctcatt
     3361 gctgtccact ccgacgcggt cgtcgccgat ttcaacggca cgcagcggct cgtggctgta
     3421 ggtgcgtcca acgaaggctg gttgctcgcg gccattatcc cctggctcaa cgggacgcca
     3481 accgccgtcc tggaaaaaca cgtaacccat cgcggcgtgc tcgtctatct gacaacggat
     3541 ggcgaaattg cgcgcatccc gaagtttgtt ggagaccttt cgcagattcg gccccgcccc
     3601 atccaaccgc ccagcggagt gaaattcgaa cggcccgccc gatacgttcc cggcccggac
     3661 gtcctgggta acgcggtgct gcagagcgac cgcgatcccg agtacgagaa tgttgccgcg
     3721 ctgggcgaag agttcatcgg ctggacactc gtcgcgaacg aggagtgcgg accggagcgt
     3781 tcgctctggc tcgaagccga tgggcgctcg cgcgagttcg gcaccgatgc ccagtcgctg
     3841 tgggcgccgg acatgaccgg gcgctatttc gacccgcaac gtctgcttcc gtccggatat
     3901 ctctacgact acgttcatgg ctacagcaag aggaccatgc tggggggctt tctgcccgca
     3961 gccgatatcg gcgtctggaa tccggaatac gaaaccggtt acgaagtgat gatggtgctt
     4021 ccgccgggtg gcgacgcgcg gcccattgcc cgtgttcgtg ccatggtgcc cgaagccgat
     4081 ggtgatcgac tgattcccga cggagatagc ggtgcacccc agaagtcgca ggccggtgag
     4141 tcggtcgacc ggtactggaa tgggtccgcc gcagatttct tcaccgccgc ggtcagcgcc
     4201 tggaaccgct ggcatcactt cttcgatgac cggatgcagg ttgagattcc cgacgagtgg
     4261 ctcctgcagg cagcgcgcgg gggtatcgcc ctctcgcgat ccagttatcg cggactccag
     4321 ccgacttatc agatcggcga gggcgcctat accaagattc cggagcggag ccacgcgctc
     4381 tttcctgtcg cccattacga atttgtctgg gcgcaccaac tttggaatct gactgcggaa
     4441 gtggagccct gcttccagtt ctatctcgac cactacatcc ttcccaacgg gaacttcctc
     4501 tacaacacgc aggatcaagt agaggccccg ctgaatgccg gcgtcttcct ggagaactcc
     4561 gcacgcgcct gggattacgc gcgcgacctc gatgccctgc gcgcgcgaat gccaattctc
     4621 cggcggatgg cggaacacgt tcggggacgc atcgcatatt cgaaaagcca tttcccggca
     4681 tcggatccgc gccacggcct cgtatggggc tctccggaag cggatctcgg tgaccccagg
     4741 gacgactatc cggaatcgca tcctttctac tatcagaatg cagcctggac ctggcggggc
     4801 tttcgcgagc acgcgcgcgc gctggcttca gcagccgcag cgcactccga tcccgcactt
     4861 ctggaggagt cgcggatcta cgccgcctgg gcaaccgacc tgcgcgccga cgtggagtcg
     4921 tctctcagca aggtgctcgc gctccgcaat cccaagatga agcaggccgg catcaccccc
     4981 ttcactccct tcgataccca tcgcaagccg acagaccttt ccagctatga gaatcaccgc
     5041 ttcatggaag actggtggat gagcgactgg ggcgatccgg aactcgacct tggtcatttc
     5101 cggcaccggg agattgccgg cctgcagatc ctgggtatga acaccgacgg cgcatatccg
     5161 cgcacgtcga actttatgtc gcacggaact ctggcgggcc gcatccggca ggaagattac
     5221 cggccttttc tgctcgcgct gtatgcgctg tgctgttaca ccatggactc cggcagtcgc
     5281 tattctccag aggacgcgat gctgcccggc ggctatcccg gcgacggcag tccctacgga
     5341 tggtcggcgg tcgtcaacag cgagttgcag gcggccttgg gacttcgctg gctcctctgc
     5401 tacgaggagc atgatcagga cgttgttcat ctccagaaag ctgcgccaaa atcctggttt
     5461 ctgcccggtg aacggattca agtgacgaac tgccccacgc gctttggaac cgtctcatgg
     5521 acaacgacga cgcggaacgg tggcggctgg aaaatgaatc tggcgctgcc acaaccgttt
     5581 gctgcggatc ttcacgtgca tatccatccg ccggacggcc gcccgctgcg cagtgcctcg
     5641 ctcggcaccg tggagaaggc ttgtgtggtt ctgcctgcgg ggctgatagc caatcggacc
     5701 aatcttgaga ttgagatctc ctgatctcgc cagcggacgc tttcgtccac gactggcgga
     5761 gcagaatttg tccatctggc gcttcaagcc agggttttac tcctaagcgc taattgtcgc
     5821 tccccaaggg gcaccccttg cggggcaccc attggttttc acctgacgcc tgggagtgag
     5881 aatgagagcg ggcactttcg gttgaagcgc cgccgtcgaa attcgtaagt tcctgatcct
     5941 atagcgcttt ctgctttcgg gaccaggggg tcggaggttc aaatcctctc tccccgacca
     6001 tttaaatcaa caatataggc ttgagcctga gggtcggaaa ctggctgggg cacccaaaag
     6061 ggcacccatt caatcaccga atcttcgcct ttttgttctt ctaaaggacg cgcgacaggc
     6121 ttggccgccg gctctgcaaa gaccgctccg acagcgcggg aaacagccgc aactccttcg
     6181 tcccaccatg attctgaata cacgttgagc gtgacatcac agtcggcgtg gcccatgttg
     6241 tctctcgtca cctccggccg tgcccccgta cgacgcatca ggctggcatg catcgtgcgg
     6301 aagctccgga agtcgatcgt ccgggacaag ccgagtttca tggccgcagg tttgatatgg
     6361 cgctgcagca gattgtgctt gtcgagcaga ttgcctgctt tgttcgcgaa gacgagattg
     6421 tcgagtccgt ggaacttcga cgactcccac gtccggcgaa gtgcgccggc aatggccccc
     6481 aggttgtcga acggaacctc tcgctcgcca gcctcggtct tcacatcgtc gaactcaccg
     6541 tcgtacaccc gttccgcgac ctggatccga tccttgagaa ttcgcttcca acggagggca
     6601 aggacctcac ccacgcgcat tgagccgaag acgatgagcg tgacgatcga cttcgttggc
     6661 tccggcaact cctcgagcat ggctcggatc tcgggcagcg ccaggagaac cggcggcttg
     6721 cggctcttct tgcgcggcag cttaaccccg actgccgggt tccgctcgag catgccccag
     6781 acaatggcct ggttcggggt gctgcgcagt gcaccgtgca tggacttgat cgtcttctgc
     6841 gacaggcgct gcgccgagtc gaacagccta ttggcctctg ggcttcttag cgccatcttc
     6901 ttcaggctga gccatctcgt cccgaagcac atagctgtgg ctcagttgct gcctcttcat
     6961 atttttgttg cgaatttcct ggaggcgggg gacgagctgt tgcgcttcat catgctgacc
     7021 gagggtgcgg cgcgcgatga tctcgcggta aagggcggtc tcgtcgtcgg gatctatttt
     7081 gagagcggct agagaggctt ccagggcgcg ctgcggctgg ctcgcctgga ggtataggag
     7141 ggcgagcaga tcgcgcgcgg gaacataatt cccgtccaga gcgacggagc gttcagccgc
     7201 ggccagagcc tctggcgacc gagggccacc cttcgtagac tgcgagaggg cttccgcgta
     7261 gaggtaccac agcaggctat cctttggatg ctgctgcgcc tgttggcgga atagctccac
     7321 cgaggcagcg aattcatgct gctgactctt catgatgcca acggcgtcgg tggccagcga
     7381 gagctgcggc ttcagccgat gcgcccgttc aaaatcggcg atggcattct cggtcttcga
     7441 gatctgcacc tcgaggacgc cacgagcgac gtagagcgaa gcggcattcg gcaggtgaac
     7501 gatgccaaag ttcaacatgt cgatgccaac ggcagcagag ctgtgattga aggcaatctc
     7561 tgcaaaattc agatagttgt cggtcctttt gggatcgagc acgatagcaa ggcgcagtaa
     7621 ctggacggcg tgaggagtat cacctgcctc ctcggcgatc tgggatccaa gcaccagagc
     7681 ggtttcgtca gtaggagggg tcagcagcgg ctccagcgcg gcgagagcct gcctgggttc
     7741 ctgcattttc cagagcacga tcgcgaggtt atagcgtgaa gtcgcatcgg gatgcgtgag
     7801 caggagttgg cgatactcct cggcggcatg cgagtagtct ccggtcgagg ccaaacacga
     7861 ggcatatccc tgggccatct caggacgcac gccgaacagg ctgcggctgg ccttgtaatg
     7921 aggcagggcg gcctggcagt cccgccgtgc gcggtcgacg gtcgccaaca tcgcgtgcga
     7981 ggttacatcg tcaggacgga tcgcgagtat gcgggtcagg agattggcgg cgtcgggctg
     8041 acggcgcgcg tactcaatct gggccgcacc ttcgagcggc agaagatcgt cgggacacga
     8101 ttgcagcgcc tgccggaagg cttgcagagc ctcgctggat cgcgccaagc cgttgagcgc
     8161 catgccgcgc agagagagca ggcgacaatc gcgcggctgc ttttgaagga gagcgttcgt
     8221 gttgctgaga gcctgctcgt actgttgcgt gcgcagcatc tgggcaatct gcggaacggt
     8281 agaaacctgt gcggacgcag tcaggactcc catcgcaacg acgcagggaa gcaaccagat
     8341 aagaaaggca cgactcttca cagcaatgtc ttttcgtacg atgcgatttt cactgtacac
     8401 caatccgcgg cagtgggatg ccggctgaat ctgatgaaag taaagaagac gggcgcaccg
     8461 ggcggcgcgc ccgtaacagg gactgcagtg ttaaaactca agccgcgcgc cgagctgacc
     8521 ctggcgcgga atctgcgtgc tggtggaggt accaaacgag gagccgctgg ccgcatcgat
     8581 attgacgccg ttcagattga cgcgattgaa gagattgaag taatcgatgc ggagctcgaa
     8641 cttcagacgc tcagtaatgt cggtggtttt cttcagcgtg gcgtcggttt cggtgaatcc
     8701 tggattacgg aactggttta tctgctcgtt gccttcctct ccgacactgg gaagcgtgaa
     8761 tggtccgcag ctatccaggt ttgtacctga gcaatgcgga aagacccctg tgatataact
     8821 gcggcggctg tgcgacatat gatagctgcc cacattgggg aaatcgttgt tatcgccgtc
     8881 ggcattgaag tcgccgctgt ccggacggaa accaaccgtg ccactgctgt ctttcgcgac
     8941 gtcgagagct ccactggagg cgacggtgaa gggcaggccg gattgcagga ccgtcgtgga
     9001 cgcgacttcc caacccgacg cgattcttcc cagaaaccca tggccggagt ttacgcccag
     9061 aagcacatag ctccagccga gcgagaagcg attggccgca tcccacgccg aaggtccata
     9121 gaagcggcta agcggatact caatgggata atttccgatg ggtaaagcgc cagagttatc
     9181 ttttgagctc gcgcgggtgt acgacgcatc gacgaagccg cggtgtgcga accgtccctg
     9241 aagagccaga atcagagcgg agtaattcga ccaggggccg ttgaaggtgt aattgattgc
     9301 gccgaagctg gtattgagac gggtctgcgt ccctgtgcag gttgccgcct ggccctggcc
     9361 ggtcggggta caggtgaggt gttgcagcag atcacccgcg tagacattga cgtccccgga
     9421 gaaggaattg ccgaaggaac tgccgcctcc atactgcagg tcgccggaat gctgaccgac
     9481 gtaacttgcg ctcgccacca tatttcgagc cagctgatgc tcgagcgcgg ctgaccaggt
     9541 ttcagtgtat ggcgacttaa gattaccgtc gacgccgccg acgccgattt gctgcccggg
     9601 caggccgcct ttcgcgtcga gcggagcccc ctggaaagcg ggatagggga agccaaaggg
     9661 atacgtgtta ctggtgccgt aaccgaagat cggtgcggcc gtcgatccgt tgttatagaa
     9721 ggtgggaagc acaaatccgg gagggttgcc cttcaggttg ttctcggcgt tgcccagcgt
     9781 gacccagtcc cgataaatac cgtaaccgcc gcgcagcacc caattaccgt tgccggtcag
     9841 atcccacgcg acgccgccgc ggggactgaa gatccagttc atcgactggt cgtagacatg
     9901 ggattgctgg cgattgacgc cattcgcgac ctgcacggcc atggttgagc cggtcccgag
     9961 atggaagtta gccaggggag tattgcggat gggatagggg ttgccgaagt tgtcgtagcg
    10021 cagaccgtag ttcaccgtga tgcggcggga tgcgcgccag gtatcctcgg cgaacacacc
    10081 gtgcgtggtt tcggcatagg catattgacc gggcattggc ttgccggtaa ccggattata
    10141 ggagaggctc gactccgaat agggattgtt attgatcaga tcgatcatgt tattgaactg
    10201 gaagctgggc tgcgcatagc tgggggcgaa aagcgcaagg tcatccccgt gccagccctc
    10261 ataaccgacg ctgatggcgt gcgtaccgtg cagtttggtg agaacgtcgc gccagtggta
    10321 gttatgctcc gcatactggc catccgcgaa accgatgccc cagccgactc caagattatt
    10381 gacgttcaca acggggacgg agagttgccc cgttgcggac gcggttccct gcaggtagtt
    10441 gtatccgaaa aatgcctgat tcagggtgtt ctgggaaaat gtgtgggttt cgctgccctg
    10501 ggtggcgatc acggacgacg ggttcgtggt attgaaggcc gggcgaactg agggaccgcc
    10561 ctcgtttgtg gtgtcgcgaa agagcgtgcc atagaggcgg tctttgctga aatatttatc
    10621 gatgcgcagg ttgtattgtt tggaattgtt gaagctcgat gagttgaaat taccgttatc
    10681 gaagacggga gtactgcagg gtatgttatc ggtactggga gtgccgcaac cggagttgcc
    10741 cgccacatcc tgcggtccaa aggtatcgag cgcggtagcg gcgacgttgg ttgtggttgc
    10801 gccgctgggt ggatacttgg ccattaactg aacttcggga gaattgggct gcacctgctg
    10861 ggcgaaggcg acgaaggcgg gatcctcgta ggtctgtacc gcgttgccgt tcgaggtaag
    10921 agcgaggtaa ggttcgatgc tgaaaaagaa gaagaactga tggtggggga ttacgggccc
    10981 acccacccca aaagacatgt tggtggtatg gaagggggcg atccgcacgg agggaggagg
    11041 gccgaattcg ccgcgagcat tgagcccctg gtaggtgtaa tagagagcgc cgaagccgtg
    11101 ataggcttct gtacccgatt tggtggtcat gaccgtctgc atggaactgg tcttgccata
    11161 atcgacggtg taggtgttgg tctgaaccga catctcggag agcgcgtccg cgttgggcac
    11221 caggctgagg acaccagggt tcacgtcgat attgatgtcg agcccatcga gtacatactg
    11281 attcccgtta ttgccgcgtc cgttggcgct gtagtcggca ttctcagtga agaagttggt
    11341 gtagcccgcg gagcccaaac ctgtgactcc gggggccaga gcaatcaatg ctgttggatt
    11401 tcgcgcagct aagggaaggg actgaacagc ctgggaatcg atcgtcagct ggttacggct
    11461 gtctgacgta tcaaggagcg ggctctgcgc gctgacagag accgtggaag cgacctgacc
    11521 gacggccagg gtgatgggaa catcgcgatt ctccgccgta ctcaggacca ggtccacctt
    11581 ggaggtcgcg aatccgtttg cggatgcgga gaccaggtag tgaccggggg caagactgac
    11641 gaagcggaag ctgccagtcg ggtcggaagt tttcgtctcc attacgctcg tatccatgtt
    11701 ggccagagtg acggcggcac cgggaaccgc attgccacgg ggatcgagaa ccgtgccctg
    11761 cacactcgcg gagaactgtg cgaacgccgg catgacatag aaaaagctgg caaaaacgag
    11821 ggaaacgata aacttcctga attgcatggt gtcggtctcc gaagtgcaac attgcctaaa
    11881 cgcagcgagc ggttggggat gcaggcgaag atgcgttgcg aatgagcctt cctgcagtgt
    11941 gaagcgaagc taatcgtttg tatcgaccgc cgcatgagcc aggctgacca ccggggcata
    12001 gtacaaatgg actctcccgc tcagggccgg gaagacgcag cgaacacgat ccggtacagg
    12061 ccaaaattca atagctccac catcgcggag gtcccgtcgc gatggatgac gagcgcctgc
    12121 tgctgctcgt cgtaaatctc gttgaccgcg agcggaccag tgccggagat gcgcgcctga
    12181 acacgcgact cgtcgagaaa ccgacaatgg actgagagca aatgcccacc ccacgggatg
    12241 cgtcttaagg cgtactccct gcccggcgca gccagcgagg agggaaaccc tggagccaaa
    12301 atcgcctgcc gcggatcgtc cgtctcgcgc agaccgacga gattgcggac gatgtgggcc
    12361 ggcagcacgc caccccatcc atagacttcg ccgccgaacg cgccatgggc tccccatact
    12421 tcgcaactca cgccaggcca gcccagtcgc ggatgtgaat cgtccccagg cgataccgag
    12481 cggcggtcca tggagggata gatccgattc gcgatcgttt ccaccaaagc agaggccagc
    12541 tgacggtcgc cactcgccca cgcggattca aggaagggca ggacgaatga tgaccagttc
    12601 agctgattgt cgggtgtcga tttcggatcg ccggactcgg cgacaaggct ttcgtacatc
    12661 ttatgcagtg tggggatcag aagcttcttc tgttcatccg tcgccatgcc gcagaaggca
    12721 ggagctgcct gcgacggatc gcggtcggcg ttgatgacca gcttcatggt gcggctgtcg
    12781 aagtcgtgaa accagtcgtt ctgccagagt tgctgcgttt tgccggcgta ggtcttctcg
    12841 atggtgcgcc actcagagac gctgccgtcg tcgccggcca gcggcgcgaa acgactcagg
    12901 atcgcgccgg cttgggacgc ggctgcctgc aactcgacca ggggcaggaa ctcgaccagc
    12961 tcgccaccgg tgggctgctg aatctggaag cggcgagagg tgtccatacc ggtttcccaa
    13021 ctgcatttgc cgacgacaaa accctgcgcg tcggtgcgat gtttgagcgt ccagcgcagc
    13081 agagccgcca gtcctggata gagtcggcgc agccaggcca ggtcccgtgt gcgatcgaag
    13141 atcgaagcgg cacagaaaaa tggaaaacac caggagatgg acgtcccgca ctccgaaccg
    13201 tcggacgcca ccatgttcat cacgccgtct tcgcgcatgc agggaatgtt gggctccaga
    13261 gcatccagaa actggccggc aaagacttct ttggccatct ccgggttcgc atagctgagc
    13321 gcccacatat cgatcgaggt ttcggcaagc acgttacgcg gcgcctggat ctgcatgccg
    13381 tcccaagggt gccgataggc gccgatgggc ctgcgcacca tggtgcgcag agtttcaaaa
    13441 tcgtagaccc aaccccgctt ccacgtttcg ggccaatcac cagcgagcac gggagccgaa
    13501 ctccagaagg ccgcgtcttc ggcgaggcga tggtcgagtt cgcgctgcgc ttcgacaaga
    13561 gaggtgcgga ccttgcgccg cagctcgccc aagttaggcg cgcgctgcat gacgaccagg
    13621 gcttcggtct gagagtgggg cgcgatgtcg agttgacagc gcatgcctcc atagagaggg
    13681 tcggtgccat aggcgacccc ggcccctgtt ttcggctcat gggtcagcca cgagccgcgg
    13741 tcggcttcct gagtcgagaa aaaatgctgg tcactacgat cgcgagctgt gatcccaaag
    13801 acggtgccgg cggcgaagcc gtgggtccac agcaggctgt tttcggcgtc gaatccgccc
    13861 gtgacgccgt cgcctcccca ccactgactg ccgccgagaa cataggtgtg cgcggcaagc
    13921 cactcaaccg accttgtgcg tccagcccgc tcctcgaact ggatctgagc ggcaatgcag
    13981 tcctcgttca cctgataaaa gctgctggtt acacgcaacc cgctttcctc gaaggcatag
    14041 accagaacat tctttgtgtg gtgcggagaa gagagctggc tggcagcgaa atcgccgggt
    14101 gagaacagga tgcgctctcc gatacggaaa ccgagcgcaa gctcggcggt atagacatgg
    14161 tttcggcgga aatcgaagta gccccccgga cccgccggat aataaagagc gaggccgatg
    14221 ccgggcaccg agcgaagcac gccgctggga tggaggtccc aggtgtgcca cggattgtcg
    14281 agatagccaa aaggcgtgta gtcatcccgg ggaaaggcag taggtgagta aggcggagcg
    14341 gaagggtggg gcgacgaaga ggcggaatgc atgcggggag cgccgagcgc acgcgagctc
    14401 cgaagcgcgg cggacgtggc cgccagcatg gaaatgaaat cccgacgatt cactgatctc
    14461 ctcttcgagc gataaaaagg ggccgtctca ggaggcacgc gagacttgca gaaccgcgac
    14521 cgaatccacc cgaatcgatg cggcaaggtc gcgactgcgg gaattataat gagcgcttgc
    14581 tgcgcccgaa tggaatgcgt ggttcatgcg caggtagtac agatggacca tgcgccgcgc
    14641 ccaccccggg ctgctgaatc aaagagagcc agaatacaaa caggacgcga acgttgcgat
    14701 acggcgcccg ccgcacgcgg ccgtcggaca ggacaggccc ggattgaaaa tggtgtttgg
    14761 gaagaatggc cgggagcgga tacatccggc ggtacgaggc tgcgtgcgcc ctctggcctg
    14821 ggcagcggga ggtcttctcc tggctctgtt gccgggatgc cggtccgatc cgccgaccat
    14881 cgctgtcatt ccccgcacta ccggtatatc gctgtgggag cccgagcacg ccggagcgga
    14941 agatgcagcc cacggagacg gtttccggat ttactggaat gcgcccaccc gcgaggacga
    15001 cgtgcagggg cagatcgcgc tggtggagcg gatcatcgat cggaaatatg ccgggctcgt
    15061 gctcgccccc gatcaaaccc tcgcgctgat gactccggtc cgcagagcgc tgtcgaagaa
    15121 gattccggtg gtcatcgtag gctccccgct accgcttcag cctggaggca agctgaccta
    15181 tatcgtcagc gacgacgctg ccgcgggacg catagccgcc gaacggatcg ggagcatcct
    15241 gaacgggaaa ggaacggtgg ccatcctcgg aattaatcca gatattgccg gcgtcctcga
    15301 gcgcgtgcgc tccttcgaga agacgctggc gcagcagtac ccggagattc aggtcgaaga
    15361 gaagcgcccc gggctcttca gtgttcccta cgagcaacag agtgccgaag aggtgctgga
    15421 gaagcatccg cacctcaatg cgattctggc ggtgacgaca gatgccaccc gcggtaccta
    15481 ctccgcgctt gtggaggcgc atcgggcggg tgaggtgaag ctggtgggct gcgaccagga
    15541 tctgctgatg ccccttatga ccggggaaat ggattccgtg atcgccgagg acacctatca
    15601 aatgggctat cgggccatcg aggtgctggc cgacgagcga gctggccgac ctgtacccag
    15661 cctcatcctc tgtgcaccgc acctggtaac aaaagagaac cttgagaccc caaagatccg
    15721 ccgggttctg ggactggtcg gaaggtgaac actgtggcag cgaacggcaa aagtcggcgg
    15781 ctgattctgg ttggcttggc ggtctgcttt ctcgccggac tcgcgacgac ggcggacctg
    15841 ctctacctgc acagttccac acgcgggctg ccgtaccacg attcctttac cgaaggaaag
    15901 gcggacgagt ggaaggcctt cggcggcacc tgggccgtgt acgacggagg aatgcgtaat
    15961 gactccgatg aacgtggggc caagctgatg accgggtccg gctattggac cgattatgtg
    16021 ttggaggcgg acatcaggct gctgggggag gacggcgatg ccggcttagt gatccgcgcc
    16081 agcgatgcgg aggagggggt ggactcctac cgcggctact atgcaggctt gcgcacgcga
    16141 gacaaccggc ttaccctggg cctggccgac catggctggc aggaatacga ggatgcgccc
    16201 attccccacg gagtgcaggc gttccagtgg tatcacctca agcttctggc ctgggactgc
    16261 gatgtggtag cttctgcaac cttcccgctg caaaacggag agacgacgac ggccgcagtg
    16321 cacgatcgcg gctgccagaa ggcgggcggc attgggctgc ggtcctattc ctccggaggc
    16381 atctggcgca acgtgcgggt ccgcagagca accgccgccg atctgcaggc gatgctggcg
    16441 gggcataccc ttcgtgagat caccagcaac cagccgtact ggaacggcac ttcaatcttc
    16501 gggtcgggat cgctgccgtc ccatgtaaat cccgaaccag ctctcgcgga agcggccgtg
    16561 cagcccatcg ccagcctgcg catggtcatt agttcgcggc cggtgacggc cacggttcgc
    16621 ggcgtagtga ctttggcttc acctgccctc tacgtccagg actccaccgg aggcgtcgcg
    16681 gttttggccg agtcgagccc tccgctcaag gtgggcgacg aagtgctcgt gaccggacag
    16741 gtgaatccac acgattacag cgccaccctc gatcatgcgg gagtgaggct gctctggacg
    16801 cgcaacccca tggcgccgat ctcgctcacc ccggagcagg ccgccaccgg ctcgttcacc
    16861 gcgatgttca tcgaattgga aggaaccttt accggcgcga cgcagacccc gcacggagcg
    16921 ccggtgctga gcctggcagc aggccagcag gccttcaggg cggtggtgga gaactcacgc
    16981 ggcagcgttc tggcgggcag gctcaaacgc ggcagcctgc ttcgtatgcg cggcgtctgc
    17041 gttgtcaatc ccgctgacac gcataacctg acccccttcg tcctcctgct gccctcggcg
    17101 gatgacgtgg aagtggtcgc cggaccaccc tggtggaaca cgcgtcatgt ggtgatcatc
    17161 ggcttcttcg tcctgctgct tgccttcatt ggccacctga tctacagcac cgtggagacg
    17221 tggcggctga aggccgtcgc ggaagagcgc gaactgctgg cgcatgagat gcacgatacg
    17281 ctcgcacaga gctttgcggg cctgggtttt cagctgcagg ccatccgtaa ccgcttgcca
    17341 gtgaacatgc cgaaggtaca ggagcaactg gacctggcct gcgatctggt gcggcacagc
    17401 cacgaagagg cgcggcgcca gatcatgatg ctgcgtgcgg agtcgcccga aaaggcagga
    17461 ctcgcccctc tgctcgagtc ctgcgcgcgt cacatgctcg aaggtggaga cgtccaggtg
    17521 gaggcggtgc aggagggcga ttgcggggcc atccctctgc ggatcaagga cacgctcttt
    17581 cgcatcggac aggaggcgct cgctaatgcc gttaggcacg cccgtccctc gcgtattcga
    17641 attctggcgc actgcgacga tgattcggtc gcgctgacga ttgaggacga cgggcgcggc
    17701 taccagcacg gcagcgaggg gaagggtttc ggcgtgcacg gcatgcgcag gagagcgggc
    17761 agcatcggag ccaatatgac cattgccaca tcgccaggcc agggtacgcg ggtggtggtc
    17821 ctcgctcccc tgccccggcg cggcacgctg agcatctggg cgggcctgct gtggaaacgg
    17881 ctgcgag
//
LOCUS       DASZYN010000492         1859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_473145, whole genome shotgun sequence.
ACCESSION   DASZYN010000492 DASZYN010000000
VERSION     DASZYN010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473145"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..629)
                     /locus_tag="VHE33_12655"
     CDS             complement(225..629)
                     /locus_tag="VHE33_12655"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HVT98349.1"
                     /translation="MAFEWDNSYSVKVAVCDEQHMKLFDIMNELANAMRMGKGREAVD
                     STLNQLLEYTQTHFRDEEALLEKTNYPQLAAHQQMHRMFVSKVQSMQTLARSGKRTSA
                     MQVLQLGREWLVNHIQKADKQYSAHLNAAGIR"
     gene            complement(859..>1859)
                     /locus_tag="VHE33_12660"
     CDS             complement(859..>1859)
                     /locus_tag="VHE33_12660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HVT98350.1"
                     /translation="AGMVNAVLRKMQRSGTGRPGERPSGSIESARFAHPAWMVERWVQ
                     RYGAAAEAICRWDQEPAALTVRADPDAVFSDLETEPGYFLTAARHVVRGDPGGSAAVR
                     EGRARIQDEASQLVAEIAACDAKRVLDLCAAPGGKTAILAERMPEATITAVDISRKRL
                     DAMRRSLPGSLAERIDFEVGDATKLDLRPEWDLILCDVPCSGTGTMARNPEIRLRVSE
                     ADLARQHVRQAAILRAGLRGLKPGGRLVYSTCSLEPEENETVVAEVLGAAAGFHRVPV
                     SGVLDRLAEYGVVTAAGRERLRSATDGQYLRTLPGVHPCDGFFAAVIQRQPIGNSQ"
BASE COUNT          324 a          691 c          487 g          357 t
ORIGIN      
        1 gctagcgcgg cgtctctgaa atctcttcac cttgtcatcc cgaccgaacc ctgagcatcg
       61 cgaagggaag cggagggacc tgcttctctt cagctttcgg aacatcacac cagcaggttc
      121 gaccgccttt gagcccctca ggaccaaaca gagcgaccgt gtcgtccgtt ctgagcgcgg
      181 atccgctccc tgaacgctga caactgagcg ctgaccctga acgtttaccg gatacccgcc
      241 gcattcaggt gcgccgagta ctgcttgtcg gccttctgga tgtgattgac cagccattct
      301 cggcccagtt gaagcacctg catggcactc gtcctttttc ccgatcgcgc cagagtctgc
      361 atcgactgca ccttggatac gaacattctg tgcatctgct ggtgcgccgc aagttgcgga
      421 tagttggtct tctccagcaa cgcttcctca tcccggaaat gagtctgcgt gtactcgagc
      481 agctggttca gggtactgtc gaccgcttcc cttcccttgc ccatgcgcat cgcgttcgcg
      541 agctcgttca tgatgtcaaa gagcttcatg tgctgctcat cgcagaccgc caccttcacg
      601 ctgtacgaat tgtcccactc aaaggccata cactttctcc cgcatgcggc cgcacgctcg
      661 gcccgcatgt ccccgagatt ggctcgctgg acccgctgcc tgacacggtc ccgtgtcgcg
      721 gcctgctacc gtatccccgc cgcattcaga tgcgcactgt actgctgatc gaccttctga
      781 atgtgattca acagttcatg catgcgcgtg gccacgaccc cggacaacca tgaggaagct
      841 ctaatgctcc ccgactggct attggctatt gcctatcggc tgcctctgga tcaccgccgc
      901 gaaaaatccg tcgcagggat gcacccccgg cagcgtccgc agatactggc catcggtagc
      961 gcttcggagc cgctccctgc ccgcagcggt cacaactccg tattcggcca gtcgatccag
     1021 cacaccagaa accggcaccc tgtgaaagcc cgccgccgct cccagcactt ccgccaccac
     1081 ggtctcgttc tcctccggct ccaaagagca ggtggaatac accagccgtc cgcctggctt
     1141 cagcccccgc aacccggccc gcagaatcgc cgcctggcgc acatgctgcc gcgccagatc
     1201 ggcctccgac acccgcagcc gaatctccgg attccgcgcc atcgtcccgg tgccgctaca
     1261 gggcacatcg cagaggatca ggtcccactc cggccgcagg tccagcttcg tcgcgtcgcc
     1321 gacctcgaag tcgattcgct cggcaagcga gccaggcagg ctcctgcgca tcgcgtccag
     1381 acgcttgcgg ctgatatcga ccgccgtgat cgtggcctct ggcatccgct cggccagaat
     1441 cgccgtcttg cctccgggcg ctgcgcacag atccagcact cgtttcgcat cgcaggcagc
     1501 aatctcggcc accaactgag aagcctcgtc ctgaatccgc gcccgccctt cgcggacggc
     1561 cgcactccct cctggatctc cgcgcaccac gtgccgcgcc gcggtcagaa aatagcccgg
     1621 ctccgtttcc aaatcggaaa agaccgcgtc ggggtccgca cgcacggtca gtgccgccgg
     1681 ttcctgatcc cagcggcaga tcgcttccgc cgccgcccca taccgctgca cccatcgctc
     1741 caccatccac gccgggtgcg cgaaccttgc cgactcgatg ctgcccgaag ggcgttcgcc
     1801 tggacggcca gtccctgaac gctgcatctt ccgcagaacc gcattcacca tgcccgcgg
//
LOCUS       DASZYN010000493        26507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1181061, whole genome shotgun sequence.
ACCESSION   DASZYN010000493 DASZYN010000000
VERSION     DASZYN010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 26507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26507
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181061"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VHE33_12665"
     CDS             <1..189
                     /locus_tag="VHE33_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98351.1"
                     /translation="VIVALAEEVKRLRLRVDVLDGQLELATRDLDEVRAFSAEDEERD
                     RYHREQAAAGFVSMGEGI"
     gene            complement(221..1045)
                     /locus_tag="VHE33_12670"
     CDS             complement(221..1045)
                     /locus_tag="VHE33_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98352.1"
                     /translation="MMQRSARWGLGSSLLLACFAASAFPARAQTGSAAGGSPATSGAD
                     PVSAISQGMTALKDNEARAALGDFQRALVSDPDNVTANLLASTAAVELYEGPAAVQYA
                     EKAEKLDPGNWKIHTTLVAAYSAAGMKQQRDRERELLRELHRSGASDARLATGFLVEM
                     FPAGANRVEAIEYFDPVGRFHTYYRFLVRQPDGKRVWEVDVQSDDFNQKSWAEAHPAE
                     AAAGERQFQITGHGDDGDGVDYRTFSGKPDYDSIRVMVVEALRAHPVTGAETPSSH"
     gene            1617..4493
                     /locus_tag="VHE33_12675"
     CDS             1617..4493
                     /locus_tag="VHE33_12675"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT98353.1"
                     /translation="MQRTFQTRIVAVLLALFTGAAIVCAGFNLVQENRYQSPTDGIWW
                     VEAPRGLEAQRVPNDSPGERAGVKPGDLVLAANGHPVHVAADVGREIWRTGAYNTIHY
                     SLERRGFRLPDVPVVLEMQDRSIHQGFRFIALVYLGIGLYVLFRRWTAPLSTHFYIFC
                     LVSFVLYAFWFTGKFNDFDWLIYWSGICAAALQPALFLHFSLAFAGERQPGRTWLDSL
                     VYIPGAAVVGLQIMAQTMWSATELLKHRLDQISVGYQALFYLAAAGVFYVHYRRAHAP
                     LERQQLKWLTRGTLLAVAPFTLFSAIPYMADFAMPDTITRIAGICLVFLPLTFSWAIV
                     RYRMMDVDLIFKRGVTYTLATAAIVGVYFALLAIAAVVAQARLRSFGTWGLFTAIIVA
                     ALLFEPLKGAIQLRVDRLFDQQSYDYRSTLISFGRGLSSFTNLDALLAAIVDRLPRTL
                     GVDRVAVFLQDGPDKFHLAANHGLPENVASAPLDLGFLNFDVRNAGTHLFLENPQLAL
                     HLSPAEQRAASLLDMNYFLPCRIGVPAVRELQVQDRTIAVIGLGRRAGGDFLSSEDME
                     LLESLAGYIGIALQNARLYASLEEKIIEYERLKEFNENIVESINVGILALDPDEKVES
                     WNSQMEVMFALPRNEALHQPLEAVFPGNLVEEFRRVKDQPGVHNLYKFRLETRAEETR
                     IANIAIAPLVSRDFRTVGRIILIDDITDQTEMEGQLAQAEKLSSIGLLAAGVAHEVNT
                     PLAVISSYAQMLARQVKGDAKLGPLLEKITQQTFRASEIVNGLLNFSRTSGAEYRETD
                     VNGIIRDTLALLEHQFKTAQIRVEMDLMPALPPIMGNAGKLQQVFLNLFLNAKDAITS
                     KAGEDGERRTLRVATEVNGHVSISIADSGSGIAPEHLRRIYDPFFTTKTARRDGQPRG
                     TGLGLAVTYGIIQEHSGKIHVESQLGSGTTFYLEFPLLRKPAHV"
     gene            4486..5922
                     /locus_tag="VHE33_12680"
     CDS             4486..5922
                     /locus_tag="VHE33_12680"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HVT98354.1"
                     /translation="MSEGTVAAPAGPQVAGPDSLPRILIIDDEAAIRESLETLLSLEG
                     YVVESVENGELGLERIAEQTFDLVLLDLALPGRNGIEILASIRERETQLPIIMITAFG
                     TVDNVVDAIRGGAQNFVQKPWDNEKLLADIRAAIARYRAEEENVQLKRALRQRYSFPN
                     IVGKSEAMLRIFDLVAQVAPTRSTVLIQGESGTGKELIAKALHANSTRKDKPFVPVNT
                     GAMPTDLLESTLFGHVKGAFTSAIAAKKGLFEVANGGTLFLDEIATMGIDTQAKILRV
                     LQDRRFMHLGGVQEIQVDVRIIAATNVDLRQAVRENRFREDLFYRLNVIAIELPPLRS
                     RREDIPALVTHFLKHYSEENGLTPRVLTPEAMRALVDYDWPGNVRELENVIERGVVLS
                     SGGSIGVDLLPGHITGSSYSSSLLEHSPDASLFDIMEDIERRIIVDKLERCNGNQTEA
                     AEQFRIPLSTLNQKIKRLNIEIRKRNRD"
     gene            6029..8266
                     /gene="recG"
                     /locus_tag="VHE33_12685"
     CDS             6029..8266
                     /gene="recG"
                     /locus_tag="VHE33_12685"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HVT98355.1"
                     /translation="MLTLETPVQFVRGIGPAIATLLAEKEIFTVEDLLLHLPFRFEDR
                     LNPRVLSELKPGETASVIGEVRGAVLLRTRKMPIFEMTVGQGIHSLKAMWFHGKYLEG
                     RFHAGQTVALYGKVDASRSGRGLKMIQPQFEILPGGNDDALDRVLEVGRITPVYESLG
                     GSKLASRWIRRTVWNILEELKGIPETLPSSLPQSLLPRLDLPSREQALREVHFPPAGT
                     ALGQLQAAATPAHKRLIFEELFYLELGLEVKRRRFQEKTGIAFETNEKVRATIREILP
                     FHPTSAQKRAFGEIAADMRRPVPMRRLLQGDVGSGKTIVALQATLIAIENGFQAALMA
                     PTEILATQHYLSARRVLEKAQHKYRITLLTGSLDDDRKRAARRRIFDGETDLVIGTHA
                     LIEEKVEFAKLGLVVVDEQHRFGVLQRFRLMKKDNAEPDVLVMTATPIPRTLALSLYG
                     DLDLSVLDELPPGRTPIVTRQVSDDRADEVWDFVRKQVKKGHQAYVVYPVIEGPKDDQ
                     PELDFALPDEVTEPEAGRKPAKTAKTETLFPKLPLKSATDMYERLREKELAGLRVGLL
                     HGRLDADEKEVVMRRFQRGEIDVLIATTVIEVGVDVPNATVMVIEHAERFGLAQLHQL
                     RGRVGRGGAKSYCILMTGGKISPGAEERLGAMVRTQDGFELAELDLELRGPGEFFGTR
                     QAGLPDFRVANLLRDRKLLELAKVEAARFVANAKEEATEEERSRVATRLKEAWQRRYG
                     LAEAG"
     gene            8372..9004
                     /locus_tag="VHE33_12690"
     CDS             8372..9004
                     /locus_tag="VHE33_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98356.1"
                     /translation="MQIRRMIVPGMAVLLIAMSARVARAGGVASAAPGQVSLQVQVGG
                     WDAPPGELNEIQRQGFHDGIEGARRDFDNHRPPDVNNRDEYRHPNLPRGQREAYRDGF
                     RRGYRVAMDHMMRGPGPQYGQPVPPPPVVRGDWNTVPGEFNDAQRRGFVDGIEGARKD
                     YENHRQPNVENRDEYRHPNMPGSLRGAYREGFRRGYDRGWAHLAGRPWRD"
     gene            complement(9232..9399)
                     /locus_tag="VHE33_12695"
     CDS             complement(9232..9399)
                     /locus_tag="VHE33_12695"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HVT98357.1"
                     /translation="MQAKKLLRALSNEESGQDLIEYALIAALIALGAIVAMGGLSSQI
                     ATEFNTIGNSL"
     gene            complement(9636..10232)
                     /locus_tag="VHE33_12700"
     CDS             complement(9636..10232)
                     /locus_tag="VHE33_12700"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HVT98358.1"
                     /translation="MQISYPSRSIVFEEGQRAQGIFVVCSGQIKLAATSRDGRTMILK
                     IAGPGDVLGLTATLNDQPYEVTAETLEPCQIRSVRRRDFLEFLNQFGEVSRQAAQVIA
                     REYHEVFLDARRLALSGSAAGRLAQLLLDWARTSSCGKPELRFTMALTHEELANMAGT
                     SRETVTRLLNQFERDELIARHGASIVILRPTEMERMVG"
     gene            complement(10674..11627)
                     /locus_tag="VHE33_12705"
     CDS             complement(10674..11627)
                     /locus_tag="VHE33_12705"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVT98359.1"
                     /translation="MILLQNNVEAGFAAPGKIVVATDLTDTEYLLPHAVAQAKASHAS
                     LILVHAVLPHESMPVETGAIPYYDPLRMDRDARLMLDSVARDIRAQGIDCIAAVRHGF
                     VPDIVAEIVRNSGAGRLILGTHGRRGLKKFVLGSVARQLLETVDVPVCTVGPRAHKKT
                     SAAPVRILHPVSLAGAHESSAALSISLGRQFAGQVILLHIIMPGTAVSRDPAAAIAAA
                     RAELEKLIPEKARAWTEARAQIAQGNVVQEILKAAQETQAELLVLGVHAPAHSWLPGT
                     EPAAYKILVSAPCPVISLKVSPAFVDEKTEDKQHSHPVVLS"
     gene            11894..11970
                     /locus_tag="VHE33_12710"
     tRNA            11894..11970
                     /locus_tag="VHE33_12710"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11928..11930,aa:Met,seq:cat)
     gene            complement(12891..14147)
                     /locus_tag="VHE33_12715"
     CDS             complement(12891..14147)
                     /locus_tag="VHE33_12715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT98360.1"
                     /translation="MTRSRYMVVVVFAIFFVMSFITNILGPLIPDIIQSFRLSYFAAA
                     LLPFSFFIAYGVLSIPSGFLVEKWGEKPVILAFFLVGLIGSILFARIHVYGIAIASLF
                     LIGSCMAGLQVAINPLLRVSGGEEHFAFNSTFAQLIFGSASFISPLIYSSIVLNLNRP
                     SQSNPILRLLAHAVPSRMQWIAMYWIFAVVIVAITLVLAFSRFPKVVRTAEESSGSLA
                     MYRSLLGRPVVWLYFLSIFLYVGSEQGTANWISEFLSHYHGFDPHTTGATAVAWFWGL
                     MTIGCLAGMILLKLFDSRVVLRVFGFGALLCLSGALFGPAAMAKVCFPCVGLFASVMW
                     PIIISLALNSVLEHQGPLAGILCSGIMGGAILPLVIGRLSDTAGLRAGMCILYVSFGW
                     VLALSFWAKPLIANQTLSRKGSLAEA"
     gene            complement(14170..15222)
                     /locus_tag="VHE33_12720"
     CDS             complement(14170..15222)
                     /locus_tag="VHE33_12720"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HVT98361.1"
                     /translation="MSQEHTNIVEGPYLLDILDQPRALHATLEHLQKDLYLLRRAAAI
                     RRQPHARVVLTGMGSSLHALYPLELALTAQGETVCRAETAELIHALPALLAPDSVIVA
                     VSQSGQSAEMIRLLDQNQRRARIIGVTNTENSPLHRKSDICILTHSGDEFSVSTKTYL
                     GSLAALEMLAAAWGGLDPEPLLAELSTAPESLASYLASWRDHVQGLASHLDDVLQYFI
                     LGRGRSLASCGTGALILKESTRTFAEGMSSAAFRHGPLEMVSPASLVLVFDGDPDTRH
                     LNHKLVRTIRECGGRAETIGSESQLTALRLPENQSRTLTLLEMAPLQMLTLAIAARTG
                     FEAGRFRHAAKITTEE"
     gene            complement(15219..16145)
                     /locus_tag="VHE33_12725"
     CDS             complement(15219..16145)
                     /locus_tag="VHE33_12725"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HVT98362.1"
                     /translation="MIAGIDVGGTKIAVGLVDTSGRVVIRQDTPIHVSRGPQDATKRI
                     LEILGALVQATGQAVTGIGIGSTGPVDPITGEMGDVNTLPGWQGWNLCTDLSSHLQVA
                     AATENDADAALLGEARVGAGKGARNLMCVIVGTGIGVGLLIDGRIYRGAGHSHPEIGH
                     HVIEASGPLCSCGAHGCWEALAAGPAMEQWFAEQSGSEAALDAKAICARARAGDPAAT
                     RAVQRTARYLGIGLANVITIFVPETMVLGGSVMDSADLWLPEIRQAVAENCRLVPHQL
                     CSISLASLGRDLGLIGAAQVWHHRFGNAQGIA"
     gene            complement(16255..19905)
                     /locus_tag="VHE33_12730"
     CDS             complement(16255..19905)
                     /locus_tag="VHE33_12730"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVT98363.1"
                     /translation="MNLRTSKMLISLGVSLFLLGLSALASAQAGRGAITGSVTDQSGA
                     VIPGAAVTLVSHATGVTLHTASDAAGLYSFVSLNPGVYRIAVSQSGFTTTVRDNVPVT
                     VDQTTAVDLALQVGSVNQVVNVSVAVNLVEPSNSTVGQLISAETIDRVPLLSRDVFDL
                     AQLSPGVTPANGTPNSSNAQAISSITSGRPGVDVSSYTINGAIIGSVYYMLDGSPLGI
                     AENNAGAIIPALVIPEDGVEETRVETQNTPASYQSGGAGVISLVSKSGGDHFHGDVFG
                     VFRPDVLSSNEYFNKQSQLTSGMSNSPPSFHRYQEGASLGGPILHKKLFFFADYEATQ
                     QATFDGSNIFTVPTSAERTGDFSADDFTIYNPTVPDNADGTRQPFANNIITNPNPIAV
                     KFLSEFPKCNMPDPKTCDSATDGATQNLYVPGVDPMTAHKFDIRLDWNQGEKQHLFGR
                     FSFDRLTSSLVNAFNSEWDPFYAQNITNGRNFLLADDLTLTPKTVVQLRYSFTRHYEN
                     QSGDPRQNGYDLTTLGFPASLAAAQNYKTLPYVFFDDVGGAIGGTANWNTFQYASENS
                     DVIASVTRISGKHELSAGFEYMKRFLNVGQPPASSGAYNFDVSATDQSTADGGGGSDF
                     ASFLIGMGETPGAESYNFTKDLFVAEASPYYSAFVHDNYHPARSLTLTAGLRWDVFGG
                     RNERHNRLEYFDPAASNTVSGVAYKGAEVYVNGDHRSPFTANLKDFGPRLGFAWQPMD
                     HLVVRGGAGFYYGPSPAAVAGASQNSDGFSSVTNWDATCYNADGNTVYNGTSACVGAA
                     PGDPDPSPTGVYSLSNPFPQGVVPLIDSPSGLGNNLGTTLNTVLHSQRTPTTYNFNFG
                     LEYEFPHQVVLSLGYVGSRGLFIPMGQIDLNTLSLETIRKYGASLCVDTSDPDCVTVD
                     NTWAPIQPGTNGNYGAATVPLWVSLQKYPQFGDGNYGSGHGVLANGYVKGDSEYSSLQ
                     AKLQKRLTSHFTTLGSFTWAKLMTDDGNPPLGFVGAHLGAAQDTQDLQYEHSVSPQDV
                     KYQFTGEVSYDLPFGRDRAVPLRGIGNAVFGGWTANGILYLGTGIPIASPVVGEPVSY
                     FNQRPNLTCDPSKGAPHTTATWFSPDCFAIPASPFVPGTAPAYLDHVRTMGARDLDVS
                     AFKSFPIGEGRTLRFDASAYNLTNHAQLGMPGVPAINDVLTQPDVAATFGQITSTVNT
                     PRQFQFGARFTF"
     gene            20072..21160
                     /locus_tag="VHE33_12735"
     CDS             20072..21160
                     /locus_tag="VHE33_12735"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HVT98364.1"
                     /translation="MAKRASGHVTLSHVARACELSISTVSIVLSEAPLSRHVAEATRQ
                     RIRAMASRLGYHPDVHARSLRRRRTQTMGVLAYDLSDPFCIPVVHGIEEALQPAQYFA
                     LQVNAQGKRKLFDRYLRMILERRAEGVIVIASWIFEEADLLADIEKNHVPIVIVGRDL
                     TARRIPSVLVDNKAGGVVAMQHLVSLGHRRIAIVRGPEELFDSEPRWQGFRCAAAEAG
                     IDLDPRLVFQLPNRLDPRSSFEGGVQFAKEIVSSGISFTAVLAFDDLTALGMIRGLTE
                     AGLRVPADCSVVGFDDILPAAVATPGLTTIRQPLEEMGQLAAERLLATLNPSDSDKKR
                     YPRLQMLAPQLVVRASTGGPQQAGPIPE"
     gene            21226..23880
                     /locus_tag="VHE33_12740"
     CDS             21226..23880
                     /locus_tag="VHE33_12740"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98365.1"
                     /translation="MLSTSLHAADLTSLHEGWRMQSACKVQATGDAISAPGFSTEQWI
                     AAAVPTTVLAAQVAAGIFPDPYFGDNLRKIPGGAYPLGEVFANLPMPADSPYRCGWWY
                     SDSFIAPASTAPGHHFWLHFGGINYRAEIWLNGHRIADSSQIAGAYRTYDLDVTDTLK
                     AGSPNVLSVEVFAPTEHDLGINWVDWNPAPPDKDMGLWGEVSLVSTGAVALRSPMVAT
                     HLPDESLQTAELTIHAELDNASDGEVNGMVSGSAAGVTFEQPVRLAAHEQRTVTFTSE
                     QFPQLRIHNPKLWWPWQMGDPHLETLTMSFRSQGQTMDEKTVQFGIREITSEFAANGG
                     GRLFRVNGKPILIRGGGWSQDMLLRTDAQRLGEQFRMVRDLNLNTLRLEGKLDTDDFF
                     NLADKNGILVMLGWCCCDHWERWQSWTPEDHTIATASLRSQMLRLRYHPSLLVWLNGS
                     DNPPPPDVEIAYLDVEAATHWPNPILSSATERATPVSGESGVKMTGPYDYVEPSYWYV
                     DHRYGGGWGYNTETSPGPAIPSLASRRKFLSDPEAWPPSADWSLHYGGGEFKNLKVLD
                     SAISAIYAPPTSVADYERMAQTMAYDSERAMFESYGKNKYVSTGVIQWMLNNAWPSMI
                     WHLYDYNLDADAGYFAVRKACEPLHIQYSYDDQSIVVVNSTYHPVSGLHASIDVHGIG
                     WNQLYSATADITVAPDSSQRVFVLPQALNNGMERILFIGLTLSDSTGKVVSRNFYWVP
                     YTLTTFNWSGTDYTHTPAERYPDLTALAHLPPATIAAHAEIVSTQQGREIQLHLENRS
                     DALAFQVHSAVRTESGDLVAPVFWSDNWIELAPGESTMLTVLLTEEGPANPVIQLDGW
                     NVQPATITPVGLRASSARTQQRGQSTRR"
     gene            25460..26479
                     /locus_tag="VHE33_12745"
     CDS             25460..26479
                     /locus_tag="VHE33_12745"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HVT98366.1"
                     /translation="MLAGKYLKPKVVEVVEVPLPDVGDHEALIKIEACGFCGSDLGIV
                     AGVHPRARAPLTLGHEFSGRIVEIRSSGSTLKAGDLVTAYPLISCGHCYVCRHGEPHV
                     CRDLRLFGIDVDGGMAEYVKLPVSSLVLLPAQMPARIGALIEPLAVAVHGVSMSPIQQ
                     PQTAVVIGAGPIGLLTALVAKARGIPRVLISDIVPSRVDLARRLGLRAVMAGEALTKI
                     LMEETGGEGADVVFECAGAPSTAREMTALVRCRGVIVNLGVFKKPAEVDLQIVNFREI
                     TIVGSRVYSRHDFEEAVQLASVLPLERIVTHSFSLRQVEAAFELFRSGQNACKVLILP
                     DGAPA"
BASE COUNT         5307 a         7941 c         8122 g         5137 t
ORIGIN      
        1 gtgatcgtcg cactggccga ggaggtgaag cggctgcggc tgcgcgtcga tgtgctcgat
       61 gggcagctcg aattggccac gcgggatctg gacgaggtgc gcgccttcag cgcggaggac
      121 gaagagcggg accggtacca ccgcgagcag gctgcggctg gattcgtgag catgggagaa
      181 ggaatctaga gccggtgctc gaactgcggc ccttccccga tcagtgggaa gaaggggttt
      241 ctgctccggt gaccgggtgc gcccgcaatg cctcgaccac catcacgcgg atgctgtcgt
      301 agtccggttt accggagaac gtgcggtagt cgacgccatc gccatcgtcg ccgtggccgg
      361 tgatctggaa ctggcgttcg cctgccgcgg cttctgccgg atgcgcttcc gcccacgact
      421 tctggttgaa gtcgtcactc tgcacatcga cttcccaaac acgtttgcca tcgggctgtc
      481 gcaccaggaa gcggtagtag gtgtggaagc ggcccaccgg atcgaagtac tcgatggcct
      541 cgacgcgatt cgcgcctgcc ggaaacatct cgaccaaaaa gcccgtagcc agccgcgcat
      601 cggaggcgcc gctgcggtgc agttcccgca gcagctcccg ctcgcggtcg cgctgctgct
      661 tcattcccgc ggcggagtac gccgcgacaa gggtcgtatg aatcttccag ttcccgggat
      721 ccagcttctc ggctttctcg gcatactgaa cggcagcagg cccctcgtag agttccacag
      781 ctgcagtcga ggcgagcaga ttcgcggtga cgttatcagg atcggagaca agggcacgct
      841 gaaaatcgcc gagcgccgct cgggcctcat tgtctttgag cgccgtcatg ccctgcgaga
      901 tcgcggagac cggatcagcg ccggacgttg ctggggaacc gccagccgct gacccggtct
      961 gcgctcgggc tggaaaagcc gaagcggcaa aacaagccag cagaagcgag gaacccaatc
     1021 cccatcgagc gctacgttgc atcatcagga gtcctgaatt ggagccttga ttcactgtag
     1081 ctcgtcacgc gagccatgaa ctcgattccc cacctgactg gggccctccc ggcagctttt
     1141 ttcaggtatg ctgaggccac cggaccccac ctcaccccgc aaggagccat ccttaatgga
     1201 attgattgtg attggcagtt tcgccgttct cttcatcagc agcattctgt gggcgacccg
     1261 ccgcccggcg gatgaacacc atcactagac ctgttgaaca tgtgaaaaag tggcagctat
     1321 cctcgctgtc cgtggtatat ggtttttcat agggtctgac aggtttccgg agaactattc
     1381 cagattgatc cgccaagggt tatgcaccgt ttcgctctcc gatcactatg taatgatgat
     1441 tcagtgacat ctgtgcctcc cctggaatgc acgattatgg atgtgcagtg gcacctgact
     1501 tgctgatcag ggacactact ggctgtcctg gactcgaaaa tccacgttct gggcatcagc
     1561 aatagttcag cagggtttct ccgcggtgtt cgttttctgg agcactgcac tctaccatgc
     1621 agcggacctt tcaaacccgc atcgtggccg ttttgctggc tctgtttacc ggagctgcaa
     1681 tcgtgtgcgc cggattcaac ctcgtccagg agaaccggta ccagtcgccc accgacggaa
     1741 tctggtgggt cgaagcgccc cggggtctgg aggcgcagcg cgtcccgaat gacagcccag
     1801 gcgagcgtgc cggtgttaag cctggcgacc tcgtgctggc ggcgaacggc catcccgtgc
     1861 atgtggctgc ggatgtgggc cgcgagatct ggcgcaccgg cgcctacaac accatccact
     1921 actccctgga acgccgcggt ttccggctgc cggacgtgcc ggttgtgctc gaaatgcagg
     1981 accggagcat tcaccaggga ttccgcttca tcgcgctggt ctacctggga atcgggctct
     2041 atgtcctgtt tcgccgctgg accgcgcccc tgtcgacgca tttttatatc ttttgcctcg
     2101 tctcctttgt tctgtatgcg ttctggttca cggggaaatt caacgacttt gactggctca
     2161 tctactggag cggcatctgt gcggcggccc tgcagccggc tctttttctc catttctcgc
     2221 tggccttcgc cggggagcgc caaccgggtc gcacctggct cgactccctc gtctacattc
     2281 cgggcgcggc cgtggtgggc ctgcagatca tggcccagac catgtggtct gccaccgaat
     2341 tgctcaagca ccgcctcgat cagatttcgg tcggctacca ggccctgttc tatctcgctg
     2401 cagccggggt tttctacgtg cactaccggc gggcacacgc tccgctggag cggcaacagc
     2461 tgaaatggct gacccgcggc actctgctcg cggttgcccc cttcacgctg ttctcagcca
     2521 ttccctacat ggccgacttc gccatgccgg atacgatcac acgcatcgcc ggcatctgtc
     2581 tggtgtttct gcccctgacc ttcagctggg ccatcgtccg ctatcgcatg atggacgtgg
     2641 atctgatctt caagcgcggt gtcacctaca cgctcgccac tgccgcgatc gtcggggtgt
     2701 acttcgcgct gctcgccatt gcagcggtcg tggctcaggc gcgcctccgc agcttcggta
     2761 cctggggact gtttacggca atcatcgtcg ccgcgctcct gttcgaaccg ctgaaggggg
     2821 ccatccagct gcgcgtcgac cgcctcttcg accagcagag ctatgactat cgctctaccc
     2881 tcatcagctt cggccggggg ttgagctcct tcacgaatct cgacgctctg ctggccgcta
     2941 ttgtcgaccg gctgccgcgt acgctgggcg tggaccgggt ggccgtcttt ctgcaggatg
     3001 gacccgacaa attccacctc gcggccaacc acggcctgcc cgagaacgta gcctcggccc
     3061 ccctcgatct cggcttcctc aacttcgacg tgcgcaacgc gggcacccac ctgttcctcg
     3121 aaaatccgca actggcactg catctctcgc ccgcagagca acgcgcggct tcgctgctgg
     3181 acatgaacta cttcctgccc tgccgcatcg gcgttcccgc cgtccgcgaa ttgcaggtgc
     3241 aggatcgcac catcgctgtc attggtctcg gacggcgagc cggcggcgat ttcctctcca
     3301 gcgaagacat ggagctgctc gagtcgctgg ccggctatat cgggatcgcg ctgcagaacg
     3361 cccgcctcta cgccagcctc gaagaaaaga tcatcgaata cgagcgcctg aaagagttca
     3421 acgagaacat cgtcgagtcg atcaacgtcg gcatcctcgc tctcgatccg gatgagaaag
     3481 tcgagagctg gaactcgcaa atggaagtga tgttcgcgct gccgcgcaat gaggcactgc
     3541 atcaaccgct cgaagcggtc ttccccggga acctcgtgga agagttccgc cgtgtcaagg
     3601 accaaccggg cgtgcacaat ttgtataagt tccggctgga gacgcgcgcc gaagagacgc
     3661 gcatcgccaa catcgccatt gccccgctgg tttcgcgcga cttccgcacc gtcggccgca
     3721 tcatcctcat cgacgacatc accgatcaga ccgagatgga gggccagctt gcccaggccg
     3781 agaaactctc ctccatcggt ttactggccg ccggcgtcgc acacgaagtg aataccccgc
     3841 tggccgtcat ctcgtcttat gcgcagatgc tggctcggca ggtcaaaggc gacgcgaagc
     3901 tcggaccgct ccttgagaag atcactcaac aaaccttccg cgcgtcggaa atcgtcaatg
     3961 ggttgttgaa cttctcccgc accagcggcg ccgagtatcg cgagaccgac gtcaacggaa
     4021 tcatccgtga cacgctggcg ctgctggagc atcagttcaa aaccgcgcag atccgtgtgg
     4081 agatggacct gatgccggca ctcccgccga tcatgggcaa cgcaggcaag ctgcagcagg
     4141 tatttttgaa cctgttcctg aatgcgaaag acgcgataac cagcaaggct ggcgaagacg
     4201 gcgagcgccg gacgctgcgc gtcgccacgg aagtgaacgg ccatgtcagc atttcaattg
     4261 cggattcggg ctccggcatc gcgcccgagc atcttcgccg catctacgat ccgttcttca
     4321 ccaccaagac tgcccgcagg gacggccagc cgcgcggaac cggtctcggc ctggcggtca
     4381 cctacggcat cattcaggaa cactcgggaa agattcacgt ggagagtcag ttgggatcag
     4441 gtacgacgtt ctatcttgag tttccgttgt tgaggaaacc cgcgcatgtc tgaaggcacc
     4501 gtcgcggctc cggccggtcc ccaggttgca ggtccggatt ccctcccgcg cattctgatc
     4561 atcgacgacg aagcagcgat tcgcgaatcg cttgagacgc tcctgtctct tgaaggctac
     4621 gttgtcgaga gcgttgaaaa tggcgaactg ggccttgaac gcatcgcgga gcagaccttc
     4681 gacctcgtgc tgctcgatct tgcgctcccg gggcgaaacg gaatcgagat tctcgcgtcc
     4741 attcgcgagc gcgaaaccca gctgccgatc atcatgatca ccgccttcgg caccgtcgat
     4801 aatgtcgtcg acgccattcg cggcggcgca cagaacttcg tccagaagcc gtgggacaac
     4861 gaaaagctgc tcgccgatat tcgtgcggct atcgcccgct atcgcgccga ggaagagaac
     4921 gtccagctca agcgcgccct gcgccagcgc tacagcttcc ccaacatcgt gggcaagagc
     4981 gaagcgatgc tccgcatctt cgacctcgta gcccaggtcg cgcccacccg ctccaccgtg
     5041 ctgatccagg gcgagagcgg cactggcaag gagctgatcg cgaaggctct gcatgcgaac
     5101 tccacccgca aagacaagcc cttcgttccc gtgaacaccg gcgcgatgcc caccgatctg
     5161 ctcgagtcga cgttgttcgg ccacgtgaag ggagcgttta cttcggcgat tgcagccaag
     5221 aagggactgt ttgaggtcgc taacggcggc actttgttcc tcgacgaaat tgccaccatg
     5281 ggtatcgaca cacaggcgaa gattctgcgc gttctccagg atcgccgttt catgcacctc
     5341 ggcggagtgc aggagatcca ggtggatgtg cgcatcatcg ccgccacgaa cgtcgatctg
     5401 cgccaggcgg ttcgcgaaaa ccgattccgt gaagatctct tctaccgcct caacgtcatt
     5461 gccatcgagt tgccgccgct gcgttcgcgt cgcgaagaca ttccggcgct cgtcacgcac
     5521 ttccttaagc actactccga ggaaaacggc ctcacccccc gcgtcctcac gccggaggca
     5581 atgcgggcac tggtggatta tgactggccc ggcaacgtgc gcgagctgga gaacgtgatc
     5641 gagcgcggcg tagtgttgtc gtcgggcgga tcgatcggcg tggacctgct gcccgggcac
     5701 atcaccgggt cgagctactc gtcgagtctg ctggagcaca gccccgacgc gtcgctgttc
     5761 gacatcatgg aagacatcga acggcgcatc atcgtggaca agctggagcg ctgcaacggc
     5821 aatcagaccg aggcggccga gcagttccgc attccactct cgacgctgaa ccagaagatc
     5881 aagcgtctga acatcgaaat ccgaaagaga aaccgcgact gattcgccgc tgcggctcag
     5941 aaaaatcggc tctccgcagt cttaagcggc gcctggcgcg tttcggcctg cacgctcatc
     6001 cccggccgat gctttagcat cacctgcagt gctcacgctt gaaactcccg tgcaattcgt
     6061 caggggcatt gggcccgcga ttgcgacgct gctcgccgag aaggagatct tcacggttga
     6121 agatctgctg ctgcatttgc cgtttcgctt tgaggacagg ctcaatccgc gggtgctgag
     6181 cgagctgaag ccgggcgaga cggccagcgt gatcggcgaa gtgcgcggag ccgtgctgct
     6241 gcggacccgg aagatgccaa tcttcgaaat gaccgtggga cagggcatcc actcactgaa
     6301 ggcaatgtgg tttcacggga aatatctcga agggcgcttc cacgcggggc agaccgttgc
     6361 gctgtatggg aaggtggatg cgtcgcgatc cgggcgcggt ctcaagatga tccagcctca
     6421 gtttgagatt ctgcccggcg gcaatgacga cgcgctcgat cgcgtgctcg aggtaggcag
     6481 aatcacgccc gtgtatgaat ccctgggcgg cagcaagctg gcgtcgcgct ggattcgtcg
     6541 aaccgtgtgg aacattctgg aggaactgaa agggattccg gaaacgctgc ccagttccct
     6601 gccccagtcg ctcctcccca gactggattt gccgagccgc gagcaggcgc tgcgcgaggt
     6661 ccactttcct cctgccggca ccgcactggg acaactgcag gcggcggcaa caccggcgca
     6721 caagaggctg attttcgaag agcttttcta cctggaactg gggctggaag taaagcggcg
     6781 gcggtttcag gagaagaccg gtatcgcctt cgagacgaac gagaaggtgc gggccacgat
     6841 ccgggagatt ctcccctttc atcccacatc ggcgcagaag cgcgccttcg gcgagattgc
     6901 tgccgacatg cggcgcccgg tcccgatgcg gcggctgctt cagggcgatg ttggttcagg
     6961 caagaccatt gtggcgctcc aggcaacgct gatcgcaatt gagaatggat tccaggccgc
     7021 gctgatggcc cccaccgaaa ttttggcgac gcaacactat ttgtccgcgc gaagagttct
     7081 ggagaaggcg cagcacaagt atcggatcac gctgctgacg ggatcgctcg atgacgatcg
     7141 gaagcgagcg gcgcgacgcc ggatcttcga tggcgagacc gatctggtca tcgggaccca
     7201 tgcgctcatc gaggagaaag ttgagttcgc gaagctcggc ctggtggtcg tcgacgagca
     7261 gcaccggttc ggcgtgctgc agcggttccg cctgatgaag aaggacaatg cggagcccga
     7321 cgtgctggtc atgaccgcca ctccgattcc tcgaaccctg gctctgagtt tgtacggcga
     7381 tctggatttg agcgtgctcg acgagttgcc ccccgggcga acaccgattg tgacccgcca
     7441 ggtcagcgat gatcgcgccg acgaggtttg ggactttgtg cgcaagcagg tgaagaaggg
     7501 acaccaggct tatgttgttt atccggtgat cgaaggaccg aaggacgatc agccggagct
     7561 ggacttcgcg ctgccggatg aggtcacaga acccgaggca ggaaggaagc cggcaaagac
     7621 ggcgaagacg gagacgctgt ttccaaagct gccgctcaaa tcggcaacgg atatgtacga
     7681 gcggctgcgc gaaaaagagc tggccggact cagggtgggg ttgctgcatg gacggctcga
     7741 tgcggatgag aaggaagtgg tgatgcggcg gttccagcgc ggcgagatcg acgttttgat
     7801 tgccaccacg gtgattgaag tcggtgtcga cgttccgaat gccacggtga tggtgatcga
     7861 gcatgcagaa cgatttggcc tggcgcagct gcatcagttg cgcgggcgcg tcgggcgtgg
     7921 cggagcaaag tcgtactgca ttctgatgac gggcggaaaa atctcgccgg gcgcggagga
     7981 gcggctggga gcgatggtgc ggacccagga cggattcgag ctggcggaac tggacctgga
     8041 actgcgcggt cccggagagt tctttgggac gcgccaggca ggacttccgg attttcgagt
     8101 cgccaatctg ctgcgagatc gcaagctcct cgagctggca aaggtcgagg ccgcgcgctt
     8161 cgtcgccaac gcgaaagagg aggcaaccga ggaggagcgg tcgcgcgtcg caactcgact
     8221 gaaggaggcg tggcagcggc gctatgggct ggccgaggcg gggtgaaact gattcagata
     8281 cagttggagt ttcggcggcg ggtgctggat cggcgagtcg aattccgaga atccgggttg
     8341 gaagaacaac agcaggaagg agaacgaatc catgcaaatc aggcgaatga tcgttccggg
     8401 gatggcggtg ttgctgatcg cgatgagcgc gcgcgttgcc agggccggcg gagttgcctc
     8461 cgcggctccg ggtcaggttt ccttacaggt acaggtcggc ggatgggatg cgcctccggg
     8521 agagctgaac gagattcaaa gacagggctt tcacgacggc atcgaagggg cgcgcagaga
     8581 cttcgataat catcggccgc ccgatgtgaa caaccgcgac gagtatcggc atccgaattt
     8641 gccgcgcggc cagcgcgagg cctacaggga tggattccgc cggggatatc gagtggcgat
     8701 ggatcacatg atgcgcggac ccggaccaca atatggacag cccgtgccgc cgccgccggt
     8761 cgtgcgcggc gactggaaca ccgtgccggg cgaatttaat gatgcacagc gtcgcggatt
     8821 tgtggacgga atcgaaggcg cccggaagga ctacgaaaac catcggcaac cgaacgttga
     8881 gaaccgcgat gagtaccgcc atccgaatat gcccggttcc ctgcgcggtg cctatcgcga
     8941 aggcttccgt cgcggatacg accggggctg ggcgcacctg gcgggtcggc cgtggaggga
     9001 ctaacaccaa tccacgtcga ggttgtacct ccaggttaca gagaagcaga caaaaagccc
     9061 ggcacatggc agccgggctt tttgtgctgg accccgccgc gatcgtttgc ggtgggatta
     9121 ctggtgattg aggaccgcgg catcgaaaag gatccgaggg ccgggcagag accgtccctc
     9181 ggatcaaagc cgatcgatgg ttttggtgtc cgtggtccgg gggaggactc cttagagact
     9241 gtttccgatg gtgttgaatt cggtggcaat ctggctcgag agaccgccca tggccacaat
     9301 agcgccaagg gcaatcaagg cagcaatcag tgcatactca atgaggtcct gaccggattc
     9361 ctcattcgaa agggcccgga gtagcttctt cgcttgcatt gctttctcct gtggtatccc
     9421 atgattcgcg tgtccatgcc ccgatggtgg agcctgtcac gactattggc tgattgaacc
     9481 gtggaaggcg ttcgccaaca atgacccgct cgtggaatta cgtcaggttg ccggggaaga
     9541 aatgacgcgc tgttcacaaa cgggacagag tccatagcgc aacacgctgc agggatggga
     9601 atgctgttac cgcagatcgc aaaagcagct atctttcagc caaccatgcg ctccatctca
     9661 gtgggccgca ggatcacaat cgatgcacca tggcgcgcga tcaattcatc ccgctcgaac
     9721 tggttgagca ggcgggtcac tgtttcacgc gaggttccgg ccatgttagc cagctcttca
     9781 tgggtaagcg ccatagtaaa gcgcagttcc ggcttgccgc aggaactggt gcgagcccag
     9841 tcgaggagca gttgagccag gcggccggcc gccgagccgg agagagcgag gcgccgcgca
     9901 tcgagaaaaa cctcgtgata ctcacgcgcg atgacctgcg ccgcctggcg gctcacttca
     9961 ccaaactgat tgaggaattc cagaaaatcg cgccggcgca cgctgcgaat ctggcacggc
    10021 tcgagggttt cggcagtcac ctcatagggc tgatcgttga gcgttgcagt caatccaaga
    10081 acatcgccgg gacccgcgat cttcaggatc atggtgcgcc catcgcgcga ggtcgcggca
    10141 agtttgatct ggccgctgca aacgacgaag atcccctgag cgcgctggcc ttcctcaaag
    10201 acgatggagc ggctgggata cgagatctgc atagcgatct catcgaaacg gtcgatggcc
    10261 tctgtcggca ggttgcagaa gagccgctcc gctcggtctg tacactgcgc gcagcggtcg
    10321 ggcacgatac gttgagttgc tgagtttccg ttttttgacg aattcgccat tagctcccga
    10381 tttctcccga ctcgctccgc gcaaaggcgc gacactgtgt actgcgactc ctgatgggcc
    10441 gctcttgttc ctcgggtatc cagcgctgga cggcgaactc agcctcgcac gcgtcactca
    10501 gccagatgag tttcagcggg aggggctggc attcatcctg gcttcccgac ccgcccatcc
    10561 ggccgacaac agtgatcccg ccgctccgca ggcagagcgc ttcggcttcg cagactggat
    10621 tgacgcaatt catcgttgac tccgaaatgc gcacaggtac aacatcgccc tcttcagctc
    10681 aacaccacgg gatgggagtg ttgcttgtct tccgtcttct catcgacgaa cgccggactg
    10741 actttgagcg agatgacagg gcacggcgcg gagacgagaa tcttgtatgc ggcaggttcg
    10801 gttcccggca accagctgtg cgccggcgca tgtacgccga gcaccaggag ttctgcctgg
    10861 gtttcctggg cggctttgag aatctcctgc accacgttgc cctgcgcgat ctgagcccgc
    10921 gcctcggtcc acgcacgagc tttctccgga atcagcttct ccagttctgc acgggccgca
    10981 gcaattgccg ctgcgggatc gcgcgatact gcggtccccg gcatgatgat gtgcaggagt
    11041 atgacctgac ccgcgaactg acgccccaga ctgatggaaa gcgccgcgct gctctcgtgc
    11101 gcgccggcca gcgaaaccgg gtgcagaatc ctcaccgggg ccgcgctggt tttcttgtgc
    11161 gcgcgcgggc caaccgtaca cactggcacg tcgacggttt cgagcagctg ccgggccacg
    11221 gaaccgagaa cgaatttctt gagcccgcgc cgtccgtgcg ttccgaggat gagtcgtccc
    11281 gcccccgaat tcctgacgat ctcggcgacg atgtcgggca caaaaccgtg gcgcaccgcg
    11341 gcaatgcaat cgatgccttg agcgcggata tcccgggcca cgctgtcgag catcagccgc
    11401 gcgtcacggt ccatgcgcag cggatcgtaa tacggaatgg cgcccgtttc gaccggcatc
    11461 gactcgtggg gcaacacggc atgaaccagg atcagcgagg catgactggc tttcgcctga
    11521 gcgacagcat ggggaagcag gtattcggtg tccgtgagat cggtggcaac aacgattttg
    11581 cccggcgcgg cgaatccggc ttcgacgttg ttctgtagga ggatcatgac ttcacctgct
    11641 ccagcgatgc gaacgctgac tgcccttagt ttcggggggt ggaagccagg cggctgtgtc
    11701 gcagcgcaca tgctcgtgtg acaccgatca cagtgtcggc acattttgta accctcgtcg
    11761 cccaggaacc gaagctgctg atagtagagg aacttgaatc gagccggcga aacttcccgg
    11821 gacgacgaga gttcacctcg ggcgaggtct gctgcggttt gaccggcttc ctgatcggtt
    11881 gctaccatgg gtatgcgggg tggagcagcc tggtagctcg ttgggctcat aacccaaagg
    11941 tcggtggttc aaatccaccc cccgcaacca tttagccgat cggccaaccc caaataaccc
    12001 catttggcat gtgcaaaacc caaaggtcgg tggttcaaat tcttctttcc taaccatttc
    12061 gccgattggc cacctccgga cagccccatt taacagcgtc ttccaggacg ccaggggcgg
    12121 gttttgctgg acggtgcctt tcacgcctac gagttcccat cctgaccatc ctgctgttgg
    12181 cctcccccct ggctgtgatc agggtgtttc ccatggcatc tttaccccgc aacgccggtt
    12241 cgggggttcg ggttggacga tccccatagg gcattctttg ccccgcagtc caccgagcgc
    12301 ctgacggggc tgttctgcag ttctatcggt tcgtgccgca cgaacaggtc aagagagcgg
    12361 catgtgttct gttagttatc ctgttcgtga atcacgaccc gcgataaatt cagaacgagc
    12421 acagagagcc ccatggatcg ccatccgcca ttgcgagagc acaccatttt ggatcatgca
    12481 gttaccgcag tccagagaac gaccagcctg gcgctgcgcg tcgttccaag gagcccttag
    12541 ctcgaaccgg agacgccatc cttgaagcgg acgcgccggc cggggcagtc cggttcgccg
    12601 ccgagatcag gaccgtccgg cacttcgaga ccattgcgat ggtccgggaa catctcgcta
    12661 gactcccacc cgatatgcac cccctgctga tcacccccta tatatgaccc gcgcgctggc
    12721 agaacgctgc cgtgctctgc agttgcactc gatctgtacc gagcgatctc cacctacgcg
    12781 gatgccggca attctggacc ggcgctacga ttctgcgact tgctcatcgg gaaattcagc
    12841 tacgatctcg aactcgccgg agcagtgcta actagagaag atgctgaggc tcaggcctct
    12901 gcgagagaac ccttgcggct gagggtttga ttggcgatga gaggtttggc ccagaacgac
    12961 agggccaaaa cccagccgaa gctcacatag aggatgcaca ttccggcccg caacccggcg
    13021 gtgtcggaca ggcggccgat gacgagcggg aggattgctc cgcccatgat gccggagcaa
    13081 aggattccag ccagcgggcc ctgatgttcg aggacggaat tgagggcgag cgaaatgatg
    13141 atgggccaca tgacggaagc aaacaatccc acgcagggga aacagacttt ggccatggcc
    13201 gcgggtccga agagcgcccc ggaaagacac agaagcgcgc cgaagccgaa gacgcgcagc
    13261 accacgcggc tgtcgaagag cttcaggagg atcatgccag cgaggcagcc gatggtcatc
    13321 agcccccaga accacgccac cgccgtcgcg ccggtcgtgt gcgggtcgaa gccgtgataa
    13381 tggctcagaa attccgagat ccagttggcg gttccctgtt ctgaaccgac gtagaggaag
    13441 atgctgagga aatagagcca gaccactggc cgacccagca gagaccgata catggcaagc
    13501 gagcctgaac tttcttcggc cgtgcgaacc actttgggga agcgcgagaa ggccagcacg
    13561 agggtgatgg ccacgatcac aacggcaaag atccagtaca tcgcgatcca ctgcatacgg
    13621 gaaggcacag cgtgggccag caggcgcagg atcggattgc tctgcgaagg acggttgagg
    13681 ttaagcacaa tcgaggagta gatgaggggg ctgataaacg aggccgagcc gaagatgagt
    13741 tgcgcaaagg tggaattgaa ggcgaagtgt tcctctcccc cggagacacg cagcagcgga
    13801 ttgatggcga cctggaggcc agccatgcag ctgccgatga ggaacaggga ggcaatggcg
    13861 atgccgtaga cgtgaatccg cgcaaagaga atggagccga tcaggcccac aagaaagaag
    13921 gcgaggatga ccggcttctc gccccacttc tccaccagaa agccggaggg aatggagagg
    13981 acaccgtagg cgatgaagaa agaaaagggg aggagagccg cggcaaagta gctgaggcgg
    14041 aagctttgaa tgatatcggg aatgaggggc ccgaggatgt tcgtgataaa tgacatgacg
    14101 aagaagatcg caaatactac aaccaccatg taccggctgc gcgtcatgaa ttcggttcct
    14161 cgctgctact tattcttctg tcgtgatttt ggcggcatgc ctgaagcggc cggcttcgaa
    14221 gccggtgcgc gccgcgatgg cgagagtcag catctggaga ggagccatct cgagcagggt
    14281 gagggtgcgg gactgatttt caggtaaccg cagggcggtg agttgggatt cggagccaat
    14341 cgtttcagcg cggccgccac actcacgaat ggtgcgcacc agtttgtggt tcaggtgacg
    14401 cgtgtcgggg tcgccgtcga aaaccaggac gagcgaagca gggctcacca tctccagggg
    14461 cccgtggcga aacgccgcgc tgctcattcc ctccgcaaag gtccgcgtgg attccttgag
    14521 gatcagcgcg ccggttccgc aggaggccag cgatcgtccg cgtccgagaa taaagtactg
    14581 aagaacgtcg tccagatgag aggccagccc ctggacgtga tcccgccagg aagccagata
    14641 gctggccaga gattcgggcg cagtggacaa ttcagcgaga agaggctcag gatccagacc
    14701 accccaggcc gcagcgagca tttccagagc ggcgagggaa cccaaatagg ttttggtgga
    14761 aacggaaaac tcgtcgccgg aatgagtcag gatgcaaata tccgatttgc ggtggagggg
    14821 actgttctcg gtgttggtga caccgatgat gcgggcgcgg cgctgatttt gatcgaggag
    14881 acgaatcatc tccgcgcttt gcccggactg ggagacggca acaatcacag agtccggcgc
    14941 cagcagggcg ggcagggcat gaatcagctc agcagtctcg gcacggcaga cggtttcgcc
    15001 ctgggcggtg agtgccagtt ccagaggata aagagcatga agggacgatc ccatgccggt
    15061 gagcacgacg cgcgcatgcg gctgccggcg gatcgcggca gctctgcgca acaggtacag
    15121 gtccttttgg agatgctcga gggtggcgtg cagggctcgc ggctgatcca gaatgtccag
    15181 gagatacggg ccctcgacaa tattggtgtg ttcctggctc acgcgattcc ctgagcattg
    15241 ccaaaacggt gatgccacac ctgggcggcg ccgatgagtc cgaggtcgcg cccgagcgac
    15301 gccagcgaga tcgaacagag ttggtgcggg acgaggcggc agttctccgc cacggcctgg
    15361 cgaatttcgg gcagccagag gtcggcgctg tccatcacac taccgccaag caccatggtt
    15421 tcgggcacga agatggtgat tacgttggcc agaccaatgc ccagatatcg ggcggttcgc
    15481 tgcacggcgc gtgtcgccgc gggatctcca gcgcgtgcgc gcgcgcagat cgctttggcg
    15541 tcgagggcag cctcactccc gctttgttcg gcgaaccact gctccattgc cggaccagcg
    15601 gcgagcgcct cccagcagcc gtgcgccccg cacgaacaga gcgggcccga agcttcgatc
    15661 acatggtgtc cgatctcagg atgagagtgt ccggctccgc gatagatgcg gccgtcgatc
    15721 agcaggccca caccaatccc cgtcccgacg atcacgcaca tcagattgcg cgcgcctttg
    15781 ccagcgccca cacgcgcctc gcccagcaag gccgcgtccg cgtcgttttc cgtggcggcg
    15841 gctacctgca ggtgcgaaga aaggtccgtg cacagattcc atccctgcca tccgggcagg
    15901 gtattcacat ctcccatttc tccggtaatc ggatcgaccg ggccggtaga tccgatgccg
    15961 atcccggtaa cagcctgtcc ggttgcctgt accagagcgc cgaggatctc caggattctt
    16021 ttcgttgcgt cctggggacc gcggctgacg tgaatcggcg tgtcctgccg gatgactaca
    16081 cgcccggagg tgtccaccag gcccaccgcg atcttggtac cgccaacgtc gatgccggca
    16141 atcatgcagt tctcctcaca tccagcagtt tctcattgcc atgccagcac ggcgccttcc
    16201 gatgactgca ggttcagcgt cgctcccgca tcgccggcct gtcgccgctc agggtcagaa
    16261 ggtgaaccgc gcaccaaact gaaactggcg tggtgtattc actgtggaag tgatctggcc
    16321 gaatgtagct gccacgtcgg gctgcgtcag gacatcgttg atagccggca caccaggcat
    16381 tcccagttgc gcatggttgg tcaggttgta ggcagaagcg tcgaagcgaa gcgtccttcc
    16441 ctcgccaatc gggaacgact tgaaggcgct gacatccaga tcgcgggcgc ccatggtgcg
    16501 gacatgatcg agataggcgg gtgcggttcc cggcacaaac ggactggcag gaatggcaaa
    16561 gcagtccggg ctgaaccacg ttgctgtcgt gtgcggagcg ccttttgacg gatcgcaggt
    16621 cagattgggt cgctgattga agtaggagac cggctccccc accaccggcg atgcaatcgg
    16681 aattccagtg cccagataca ggatgccgtt ggctgtccag ccgccgaaga cggcgttgcc
    16741 gatgccgcgc aacggaacgg cgcggtcgcg gccgaacggc aggtcatagg aaacctcacc
    16801 ggtgaactgg tatttcacgt cctgagggct tacggaatgt tcgtactgaa ggtcctgtgt
    16861 atcctgagct gcacctaagt gggcaccgac aaatcccaga ggcgggttcc cgtcatcggt
    16921 catcagctta gcccaggtga agctgccgag agtggtgaag tggctggtga gcctcttctg
    16981 cagtttggcc tgcagggaac tgtactcaga gtcgcccttc acatagccat tggcgagtac
    17041 gccgtggccg gagccatagt tgccatcgcc gaactggggg tacttctgca gtgaaaccca
    17101 cagaggaact gtggcggcgc catagtttcc attcgtgcct ggctgaatcg gcgcccaggt
    17161 attgtcgacc gtcacacagt caggatcgga tgtatcgacg cagagagagg cgccgtactt
    17221 ccgaatcgtc tccagactca gggtgttcag atcgatctga cccatcggga tgaagagccc
    17281 gcggctaccg acatatccga ggctaagcac aacctgatga ggaaactcat attcaagacc
    17341 aaagttgaag ttatatgttg tgggcgtgcg ctgcgaatgg agaacagtat tgagcgttgt
    17401 tcccaaatta ttgcccagcc cggatggcga atcgatcaga ggcaccacgc cctggggaaa
    17461 cggattactg agagagtaga cgccggtcgg actcggatcc ggatctccgg gcgcggcccc
    17521 cacgcatgcc gaggtgccgt tgtacacggt attgccatcg gcgttgtagc aggtggcatc
    17581 ccagttcgtc accgaggaaa aaccatcgct gttctgagag gcgcccgcga ccgccgctgg
    17641 gctcggccca tagtaaaatc cggcgccacc gcgtacgaca agatgatcca tgggctgcca
    17701 ggcaaagccg agccgcggac cgaagtcttt caggtttgcg gtgaaaggag accggtgatc
    17761 gccgttgacg tagacctcag cgcctttata ggcgacgccg ctaaccgtat tcgaggcagc
    17821 agggtcgaaa tattccagac gattatgcct ctcgtttcgt cccccaaaga cgtcccaacg
    17881 taagccggcg gtcagggtaa gagacctggc aggatggtag ttatcgtgca caaaggcgga
    17941 ataatagggg ctcgcctctg cgacaaacag atctttggtg aagttatagc tctcggcgcc
    18001 cggcgtctcc cccattccga tcaggaaaga cgcgaagtcg ctgccgccgc cgccatcggc
    18061 cgtcgactgg tccgtggcgc tgacgtcgaa gttgtacgct ccggaagacg cgggtggctg
    18121 cccgacattc agaaagcgct tcatgtattc gaatcccgcg ctcagttcat gtttgccgct
    18181 gattcgggtc acactggcga taacatcgct gttttcgctc gcgtattgaa atgtgttcca
    18241 gttagccgtg cctccgatgg cgccgccaac atcgtcgaag aacacataag gaagagtctt
    18301 atagttctgg gctgctgcca gcgatgcggg aaagcccaga gtggtaaggt catagccgtt
    18361 ctgcctcgga tcgccactct ggttctcgta gtgccgcgtg aatgagtagc gcagttgcac
    18421 cactgttttc ggggtgaggg taagatcgtc ggccagcaga aaattgcgcc cgttcgtgat
    18481 gttctgcgcg tagaacgggt cccattcact attgaacgcg ttcaccagcg aactggtcag
    18541 ccggtcgaag gaaaagcggc caaagaggtg ttgtttctcg ccctgattcc agtccaggcg
    18601 aatgtcgaac ttgtgcgcgg tcatcggatc tacgcccggc acgtagagat tctgggtggc
    18661 cccatcggtc gccgagtcgc aggtcttcgg atccggcata ttgcactttg ggaactcgga
    18721 aagaaacttc acagcaatcg gattgggatt ggtgatgatg ttgttcgcaa acggctgacg
    18781 ggtaccatcc gcattgtctg gaaccgtagg attgtagatg gtgaaatcgt cggctgagaa
    18841 atcgcccgtt cgctcggcgc tggtgggaac cgtgaagata ttcgagccgt cgaaggtggc
    18901 ctgctgggtt gcttcgtaat cggcgaagaa aaacagcttc ttgtgcagga tcgggccgcc
    18961 cagcgacgcc ccttcctggt agcggtggaa agagggcggt gagttcgaca tgccgctggt
    19021 caactggctt tgcttattga agtattcatt ggacgacaga acatcgggac ggaagacacc
    19081 aaaaacgtcg ccgtggaaat gatcgccgcc ggacttgctc accaggctga tgacgccggc
    19141 tccgccgctc tggtatgacg caggagtgtt ctgcgtctcg acgcgggttt cttccacgcc
    19201 gtcctcggga atcaccaggg cgggaataat ggcgccggca ttgttctccg cgatgcccag
    19261 ggggctgcca tccagcatgt agtaaaccga gccgatgatg gcgccgttga tggtgtagga
    19321 cgatacgtcc acgccaggcc ggccgctggt gatgctcgag attgcctgcg catttgagga
    19381 gttcggcgta ccgttggctg gggtcacacc cgggctgagc tgcgccaggt caaagacgtc
    19441 gcgcgaaagc aggggaacgc ggtcgatcgt ctcggcggaa atgagctgcc caaccgttga
    19501 gttgctgggc tccaccagat tgaccgcgac gctgacattc actacctgat tcacgctccc
    19561 cacctgcaac gccagatcga cggcggtcgt ctgatcgacg gtgacaggaa cgttgtcgcg
    19621 gactgtcgtt gtgaagccgc tctggctcac ggcgatgcgg tagacgcccg gattgagaga
    19681 aacgaaggaa tacaggcctg ccgcatccga ggcagtgtgc agggtgacgc cggtcgcatg
    19741 gctgaccagg gtgacggccg ctccgggaat gacagcgccg gactgatcag tgaccgagcc
    19801 agtgatggcg ccacggccgg cctgggcgga ggcaagcgcg ctcaggccga gcagaaacag
    19861 cgagacgcca agagagatga gcatcttcga agttcgtaga ttcataggcc gcctcccgtg
    19921 agtcttgata ttcaaacgtt tgaatggtat ataaatttag gaagaacaga tcattatgtc
    19981 aagcggaaaa tcgtcccccg ctctccacgg gcttttcccg accatgtacc cgcgtccgac
    20041 ataatcgact acggaagaag gaggaaacga cttggcaaag cgcgcatccg gtcacgtgac
    20101 cttgtcccac gtcgcacgcg cctgcgagct gtccatctcg accgtgtcga tcgtgctcag
    20161 tgaagcgcct ctttcccgcc acgtggcgga agccacacgc cagcgcatcc gagcgatggc
    20221 cagccgcctc ggctaccatc ccgatgtcca cgcgcgttcg ctgcgccgtc gtcgaacgca
    20281 aactatgggc gtgctggcat acgacctttc cgatccgttc tgtatcccgg tggtgcacgg
    20341 cattgaggaa gccctgcagc cggcgcagta tttcgctttg caggtgaatg cgcaggggaa
    20401 gcgcaagctc ttcgaccgct atctgcgcat gattctggag cggcgcgccg aaggcgtcat
    20461 cgtgatcgcc agctggatct tcgaagaagc cgatctgctc gccgacattg agaagaacca
    20521 tgtcccgatc gtgatcgtgg ggcgcgatct taccgctcgc cggatccctt cagtgctggt
    20581 ggataacaag gcaggcgggg tcgtcgccat gcaacacctg gtttcgctcg gacaccggcg
    20641 catcgcaatc gttcgtggcc cggaagagct gttcgatagt gaaccgcgct ggcagggttt
    20701 ccgctgcgcg gctgccgaag ccggcatcga ccttgatccg cgactggttt tccagcttcc
    20761 caatcgactc gatccgcgat ccagcttcga aggcggagtg cagtttgcca aagagatcgt
    20821 gtcctcagga atctccttta cggctgtgct tgcctttgac gacctgactg cactgggaat
    20881 gattcgcgga ctgaccgagg cgggactgcg tgttcccgcc gattgctcgg tggtcggctt
    20941 tgacgacatc ctgcccgcag ccgtcgccac ccccggtctc acaaccattc gtcaaccgct
    21001 cgaagaaatg ggtcagctcg ccgccgagcg gctgctcgca actttgaatc cgagcgacag
    21061 cgacaagaag cgctatccgc gtctgcagat gctcgcgcca cagctggttg tgcgagcctc
    21121 caccggcggg ccgcagcaag cgggcccgat tcccgagtga gttcacgagg gaaaggtctt
    21181 aacgccatga atcgatcgtt cctcttttgt gtttgcgcat ccgcaatgct gtccacctca
    21241 ctgcacgccg ccgacctaac ttccctgcac gaaggctggc gcatgcagtc tgcctgcaaa
    21301 gtgcaggcca ctggagatgc catctccgcg cccggctttt cgactgagca gtggattgcc
    21361 gccgcggtgc ccaccaccgt gctggccgcg caggtcgcgg ccggcatatt tcccgatccc
    21421 tacttcggcg acaacctgcg taagattccc ggcggcgcct atccgctcgg cgaagtcttc
    21481 gcaaacctgc ctatgcccgc cgatagcccg tatcgctgcg gctggtggta cagcgattca
    21541 ttcatcgcgc cggcctccac agcaccgggc caccatttct ggctccactt tggcggcatc
    21601 aactatcgcg cagaaatttg gctcaacggc cacaggatcg ccgactcctc gcaaatcgcc
    21661 ggcgcttatc gcacctatga cctcgacgtg accgacacac tcaaggccgg ctcccccaat
    21721 gttctctccg tggaagtctt cgctccgacc gagcacgacc ttggtatcaa ctgggtggac
    21781 tggaatcccg cgcctcccga caaggacatg ggcctgtggg gggaggtcag tctggtgagc
    21841 acgggtgcgg tcgcgcttcg ttcgcccatg gtggcgacgc acctgcccga cgagagcctg
    21901 cagacggcgg agctcacgat ccatgcggaa ctggacaatg cgtccgacgg cgaagtgaac
    21961 ggcatggtct cgggctcggc ggccggcgtc acgttcgagc agccggtgcg tctggctgcg
    22021 cacgagcaac gcaccgtcac cttcacctcg gagcagttcc cgcaactacg catccacaat
    22081 ccgaaactct ggtggccctg gcagatgggc gatcctcatc ttgaaactct caccatgagc
    22141 ttccgcagcc agggtcagac gatggacgag aagacggttc agttcggcat ccgcgaaatc
    22201 acttcggaat tcgcggcgaa tggcggcgga cgcctcttcc gtgtcaacgg caaacccatc
    22261 ctcattcgag gcgggggctg gtcccaggac atgctgctcc gcaccgatgc acagaggctg
    22321 ggcgagcagt tccggatggt gcgggatctg aacctcaata cgttgcgcct cgaaggcaaa
    22381 ctcgacaccg acgacttctt caatctcgcc gacaagaatg ggattctcgt catgctcggc
    22441 tggtgctgct gcgatcactg ggaacgctgg cagagctgga cccccgaaga ccacaccatc
    22501 gccaccgcgt cgctgcgctc acaaatgctg cgcctgcgct accatcccag cctgctggtc
    22561 tggctcaacg gcagcgacaa tccgccgccg ccggatgtcg agatagccta tctcgatgtc
    22621 gaagctgcca cgcactggcc caacccgatc ctttcttccg cgacggaacg ggcgacccct
    22681 gtgtcgggcg agagcggcgt aaagatgacg ggtccgtatg actacgtcga gcccagctac
    22741 tggtatgtcg atcatcgcta cggcggcggc tggggataca acacggaaac cagcccgggc
    22801 ccggcgattc cctcgctcgc cagccgcagg aaattcctgt ccgatccaga ggcatggccg
    22861 ccctctgctg actggagctt gcactacgga ggcggcgaat tcaaaaacct caaagtcctg
    22921 gacagcgcga taagcgcgat ttatgccccg cccacttccg tcgcggacta cgagcgcatg
    22981 gcgcagacca tggcctacga ctccgagcgc gcgatgtttg agtcctacgg caagaacaag
    23041 tacgtttcca ccggcgttat tcagtggatg ctgaacaacg cgtggccgtc gatgatttgg
    23101 cacctctacg attacaatct ggacgccgat gcggggtact tcgcggtcag gaaggcgtgc
    23161 gagccgctcc atatccagta ctcgtacgac gaccagtcga tcgtagtcgt caacagtacc
    23221 taccatccgg tttccggcct gcacgccagc atcgatgtgc acggcatcgg ctggaaccag
    23281 ctgtacagcg caacggcgga catcaccgtc gcgcctgaca gctcgcagcg ggtcttcgtc
    23341 ctgccgcagg ccctgaacaa cgggatggaa cgaatcttgt ttatcggcct caccctcagc
    23401 gactccaccg gcaaagtcgt cagccgcaac ttctattggg tcccgtacac gcttacgacg
    23461 ttcaactggt cggggactga ctatacgcac acgcccgcgg aacgctatcc ggatctcacc
    23521 gctttggctc atctgccgcc ggcgacgatc gccgcccatg cggagatcgt cagcacgcag
    23581 cagggccgcg agatccaatt gcatcttgag aaccgctccg atgcgctggc cttccaggtg
    23641 catagcgcgg ttcgcaccga atccggcgac ctggtcgcgc ctgttttctg gtccgacaac
    23701 tggatcgaac tggctccggg cgaatcgaca atgctcaccg ttctgctgac ggaagaaggc
    23761 ccggcaaatc ccgtaatcca gctcgatggc tggaacgttc agccagccac catcacaccg
    23821 gttggcctgc gtgcatcttc agcgcgcact caacagcgag gccaatctac acgccggtga
    23881 ttcgctctcc cggggcagcg ctcagcggta acatttacaa gtcgcgaaaa ctgcggactc
    23941 gacccggatg tgttgagcca gcactttcca tataggggaa aacaaaaacc gttccttgat
    24001 gaaatgtgct agccatctcg aggacagagc cagcggcagg ctgggcaagc cacgaaggag
    24061 aatcctcgtt tgccctatgc gaccggctcg accggactcg cgctaacagt cagaggcatc
    24121 tcccgacgga tcatgattat gcggttccga cccgcgaata tcagactgat caatcgtcgc
    24181 tcaaatctgc ccagtctgct gcttgctctg tcctctgaga aaggcaacat caaaaacaac
    24241 cccaccacat ccgcggattg acagggtcac tccatatcag gccaaatccc ttacgcactc
    24301 caattagccg tcaggtgatt ccgatgcgca ccgaaacttg gtgggttccg ccccatggcc
    24361 gccgtcactc gtcggtttca acccaaacca accccaaata acagggcaga agtcctctct
    24421 atgagtattt agagctcttt acaaacccca accacgctgt ccaactcatt gaaactacgt
    24481 tgattgttgc cctcacaacc caaaggtctt gtgttcaaat ccaccccccg cgaccatccg
    24541 aatcaaagac ttacgtcttt aggtcccttc cggcacactc ccaaaaactc ccagttcgcg
    24601 cccaatagtc gctcggtttc ggaccttcca aactccaacg acctaggcga ttgcagcgca
    24661 tcttgtgctc gtgggggctt tcaaagtcca aaatagtgcc ctcgggccgt tgatctggtt
    24721 cgcggggcac gaggcctcga atcaatcaat tactctgacg tggctagtcc gacggactcc
    24781 cggaacctct cgcgcagcgg agatcgcatc tcgcattccg tgcacgcgga agcctggagc
    24841 gggcgcttcg tttcgttcgc aattcggcgg gccctgcgtc cagccgtcgg cgatcgggct
    24901 gaggagaccg gcaacgagaa tcgtggcgtc agaaactggc gacaagcccg tttttcagta
    24961 cctatttgaa gccggcctga aagccggtct cggtgagcgc attggcctga cgtcaacagt
    25021 tgagcatcga gacctgctgc tcgccggaca caagccggat tacgagattg gcaatcttag
    25081 gtttggccca ctgaatctgt ggcatccccg gtccccagcc tccaactgcg atggaacggg
    25141 ggctctctcg attgtggtct ggaaagaggg aattggaccg ggcccgcgcc ccctgcggac
    25201 tgggcgccac gagcgattag acgggctgcc cgcagtagca gtcgttatta gagttagggc
    25261 ggtgatgtcg tcttcctggc aaactgctcg gcaggaatca ggaagaactg atctggtgat
    25321 gcgtcccgca gaaatgaatg tggcaggact ccagaagtcc ttcgatttca cagcgccacc
    25381 attttttcct tgccgcaatt tcttgacgat gatagagaat ctataagaga atgatttggg
    25441 tgacgggagc gtccagacgg tgctcgcagg taagtatctg aaaccaaagg tggtcgaggt
    25501 cgttgaagtt cccttgcccg atgtcggcga tcatgaagcc ctgatcaaga ttgaggcctg
    25561 tggtttctgc ggctctgatc tgggcattgt tgccggtgtc catcctcgcg cgcgtgctcc
    25621 cctgacactc ggacacgagt tttccggacg tatcgtcgag attcgcagtt caggctccac
    25681 cctgaaggct ggagatctgg tgaccgctta cccgctcatc tcctgcggcc attgttacgt
    25741 atgccgtcat ggggaaccgc atgtatgccg ggatttgcgg cttttcggga ttgatgtgga
    25801 tgggggcatg gcggagtatg tgaagctgcc ggtttcgagc ctggtgctgc tgcccgccca
    25861 gatgcctgct cgaattggag cgttgatcga gccgctggca gttgcggtgc atggggtttc
    25921 catgtcgccg attcagcaac cacagaccgc tgtggtgatc ggggctggac cgatcggctt
    25981 actgacggct ctggttgcga aggcgcgggg cataccccga gtcctgatca gtgacattgt
    26041 gccttcccgt gtcgatctgg cgaggcgttt gggtttgcga gcggtcatgg cgggagaagc
    26101 gctgacgaag atcctgatgg aggaaaccgg cggcgagggc gccgatgtgg ttttcgagtg
    26161 cgcaggagca ccgtcaaccg cgcgggagat gacggcgctg gtgcggtgcc ggggagttat
    26221 cgtcaatctc ggagtcttca agaagccggc cgaggtcgat ctccagatcg tgaacttcag
    26281 agagatcacg atcgtcggtt cgcgggtcta cagccgccac gattttgaag aggccgtgca
    26341 actggcgtct gtgcttccac tggagcgcat cgtgacgcac tccttctctc tgcgccaggt
    26401 tgaggcggca ttcgagctgt ttcgcagcgg acagaatgcc tgcaaggtgc tgatcctgcc
    26461 cgatggagcg cctgcatgaa tccgttttcg gttgccggcc ggctggc
//
LOCUS       DASZYN010000494         6708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_537578, whole genome shotgun sequence.
ACCESSION   DASZYN010000494 DASZYN010000000
VERSION     DASZYN010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6708
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537578"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3272)
                     /locus_tag="VHE33_12750"
     CDS             complement(<1..3272)
                     /locus_tag="VHE33_12750"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT98367.1"
                     /translation="MNGRNRVYWVVLFLAGLFFAGSPLAHGQYENGSLVGTVRDSSGA
                     VVPRTAVSVTNTATGITEKTTSNDQGDYEFPSLRTGVYTINASQQGFAPAIARNITIS
                     VGSRQRIDLRLVPGQTETTVEVSDVALQLETDSSQRGQTITNYQTEALPLVSRNYSDL
                     LGVVTGARQAPSQATTSSISSLVRAGSYNVNGQRSMFNNFLLDGMDNNAYGESNQGFD
                     NQIIAIPPDSVAQFSVVTNNESAEYGRGSAATVNVASASGTNAYHFTLYEFLRNTDLN
                     AAGFFKPNVVSNTGKVTPFHKPAFNRNQFGMNFGGPILRDRLFYFLDYEGFRQTLTPL
                     SVLTLPTQNELNGILVVPVRNPLTGTVYPAGTAIPNTAIDPLNAQVVNYFKKLPLPQA
                     GLATTGLNSNDYSVEVPFTDNSDKGDLRLDYQQNAKSSWFLRISDRKETGVNYTTIPL
                     PLDGQTNGTIRILDQQVALGNTYVFGPNKILDARLGLSRTKAGKFNLSIGDNAFTIPG
                     LPSNPVVAGGLPSVGITGFNGFGRQSTNPQWQNPALIDPKLNFTWVKGKQSLKFGYEY
                     EHIWMAVNDNNPLYGSWTYGGGYSLCDAAISASCATTSSGAAAPTAPVSDTYWADFLF
                     GTTSSYQLANYFVVHLRQSLHSAYAQDDWRVSPKLTLNLGVRWEYGSPYSDWKNYISN
                     WDPASQTVLTIAPGATTGNGITVVHPGGVYGSTLVNPDLNDFMPRIGFAYAPWTKTAI
                     RGGFGTGYVHYTRAGSGDILAINAPQAQFAVVTQIKPSTTNQCSTPLPAQIIPTGSTT
                     PSCYATASQGYPSGLVTAFNPATDNITWIPKDRPDSYVESYFLSVQQQLAKNSLVDIA
                     YVGNHGLHLEGFMNGNQKDPSKGFARPFTNWPSDITEAYNEFFSHYNALQVRYEQRSV
                     AGLTLLNSFTWEHAMDNAAASLEANTPSPQNAYNLRGDYAQSDYNLPIADTLSLVYDA
                     PFGRGRQFLSHLNGAGDALLGGWQISAINSMQSGTPFNLTYTPNTAQQVSQQISATYR
                     GANEYRPDIVPGKKVTQGTSSRAANTGYVNYVNYSAFVLPPVKDAAGNALSPFGNASR
                     NPG"
     gene            3347..4084
                     /locus_tag="VHE33_12755"
     CDS             3347..4084
                     /locus_tag="VHE33_12755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT98368.1"
                     /translation="MCKIVTDMDSGPSPTPRRILAVDDDPVSLAVTAVLLEAEGCVVL
                     QAESGVQALDLARSDPPDCILADLRMPGLNGPALARSLRKAAPRALLLAMSATPPPEL
                     DGYDAVLKKPLSPESLRSAFARRPVPAPPAPKSPKEDAVLDADIFERLRRAITPEGLE
                     EVFTVFLSDTHQRIAAMRKADASTIRKEAHTIKGGASMVGALQISRAAAVLESGVDDP
                     DDRLRKLDEMEAHCRFAAIILKQRLKA"
     gene            4081..4800
                     /locus_tag="VHE33_12760"
     CDS             4081..4800
                     /locus_tag="VHE33_12760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT98369.1"
                     /translation="MNSPTKSARPAVPARPSKSAPPIRILIADDHPVIRIGVRNMLQA
                     EGGLDVVGECSDGDEAITQTLELLPDILLLDVYMPRLPGIEAMRAIMNGSPSVKIILL
                     TSTISTQQIIEALQIGARGIVIKDVVAEHITAAIRAVAGGDYWIGGKRVVNLVGALHD
                     LMQQAAVPDRKTFGLTPREMEVVGCIVEGCSNRDIAKQFGLSEETVKRHLSNIFDKTG
                     VSTRLELAMFAIAHHLVATEH"
     gene            4975..5790
                     /locus_tag="VHE33_12765"
     CDS             4975..5790
                     /locus_tag="VHE33_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98370.1"
                     /translation="MMQASSVLLCCVFLASLTPAAHTQNPPSASSVQLTPYTTPDQSA
                     SAGVPSGWKATGSQTTITLTGPQNESITLGRVYIARNAPFQAGQRPGGGADLAMPYNA
                     PLTQKLIAVYQQAYALAGKPVPQIAFTSTTPLQVPSILGQCGRFVANLTGAGDPAKIM
                     GIFCSFPLDSAGAFKNFILVAQAPASTAAQEAPLAQAVFSSYRIPQAMLARKLAPVTA
                     PPPPSSGPGAGMSSTLWGMRQADIMATCMDEGVIREYGPRQLPRECGGLAPNP"
     gene            5870..>6708
                     /locus_tag="VHE33_12770"
     CDS             5870..>6708
                     /locus_tag="VHE33_12770"
                     /inference="COORDINATES: protein motif:HMM:NF012872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98371.1"
                     /translation="MPSEQVAAVEAGHNTTWSRNNPYSATVLENRLLTGSGSEKETRH
                     IEFDLADSGITYLPGDSAGILPINAQDAVDLVLERLTLTGQEPVTDFYGKPLDLRQAL
                     TSWLMIGKVSGSTLKAWAKHTGNAELSDIAANREKLDAYLWGREFLDLLEHAPGKIED
                     PQQLFKILPRLAPRLYSISSSQALLGTQIDLCIRVVKYNTHGRDRHGVCSTHVGERTP
                     KGEKLPIFIHSNQLFRLPADPDARVIMVGPGTGVAPFRAFLQHKQAGQGRWPMWLVFG
                     DQRA"
BASE COUNT         1213 a         2457 c         1737 g         1301 t
ORIGIN      
        1 cccggattcc gcgaagcgtt tccgaagggg ctcagcgcat tgcccgccgc atctttcacc
       61 ggcggcagca caaatgccga gtagttgacg tagttcacgt aaccggtgtt ggcggcgcgc
      121 gagctggtgc cctgcgtcac cttcttgccc ggcacgatat ccggccggta ctcgttcgcg
      181 ccgcgatacg tcgccgagat ctgctgcgaa acctgctggg ccgtgttcgg cgtgtacgtc
      241 aggttaaacg gcgttcccga ctgcatggag ttgatcgcgc tgatctgcca tccgcccagc
      301 agcgcgtccc ccgctccatt cagatgcgac aggaactgcc gtccgcgccc aaacggcgcg
      361 tcgtacacca ggctcagcgt atccgcgatc ggcaagttgt aatccgattg cgcgtagtcg
      421 ccgcgcaggt tgtaggcgtt ctgcggcgac ggcgtgttcg cctcgaggga cgctgccgcg
      481 ttgtccatcg catgctccca cgtaaacgaa ttcagcagcg tcagtcctgc caccgatcgc
      541 tgttcgtacc gcacctgcag cgcattgtag tgagagaaaa attcgttgta ggcctccgtg
      601 atgtcgctcg gccagttcgt gaatggccgc gcgaatccct tcgaaggatc cttctggttg
      661 ccgttcatga atccttccag atgcagcccg tgattgccca cgtatgcaat gtccaccagc
      721 gagttcttcg ccagttgctg ttgcacgctc aggaagtagc tctccacgta gctgtccggc
      781 cggtccttgg gaatccatgt gatgttgtcg gttgccggat tgaacgccgt gaccagtccc
      841 gacggatagc cctggctcgc cgtcgcgtag caactgggcg tcgtcgaacc cgtcgggata
      901 atctgcgccg gcagaggcgt gctgcactgg ttcgtcgtcg atggcttgat ctgtgtcacc
      961 accgcgaact gcgcctgcgg tgcgttgatg gccagaatgt ctccggaacc cgcgcgcgtg
     1021 tagtgcacat atccggtgcc gaatccgccg cgaatggccg tcttcgtcca cggtgcatac
     1081 gcaaatccga tccttggcat aaagtcgttc aggtccggat tcaccagcgt gctgccgtac
     1141 actccacccg gatgcaccac cgtgatcccg ttcccggtcg tcgcgcccgg cgcaatcgtc
     1201 agcaccgtct gcgacgccgg atcccagttc gaaatgtagt tcttccagtc cgaatagggc
     1261 gagccgtact cccaccgcac tcccaggttc agcgtcagct tcggcgaaac tctccagtca
     1321 tcctgcgcat aagcgctgtg caggctctgc cgcaggtgga ccacgaagta gttcgccagc
     1381 tgatagctgc tcgtcgtgcc gaacagaaag tccgcccagt aggtgtccga cacaggcgcc
     1441 gtcggcgcgg ccgctccgct ggacgtcgtc gcgcaactgg ccgagatcgc cgcatcgcac
     1501 aggctgtagc cgccaccgta cgtccacgat ccgtacagcg gattgttatc gttcaccgcc
     1561 atccagatgt gctcgtattc ataaccgaac ttcagcgatt gtttcccctt cacccacgtg
     1621 aagttcagtt tcggatcaat cagcgccgga ttctgccact gcggattcgt gctctgtcgt
     1681 ccaaacccgt tgaagcccgt gatgcccacc gagggcagtc cgcccgcgac cacgggattc
     1741 gacggcagtc ccggaatcgt gaacgcgttg tccccgatcg acagattgaa cttgcccgct
     1801 tttgttcgcg acagccccag ccgcgcatcc agaatcttat tgggcccgaa cacgtacgta
     1861 ttgcccagcg ctacctgctg atccagaatg cggatcgtcc cgttcgtctg cccgtccagc
     1921 ggcaaaggaa tcgtcgtgta gttcacgccc gtttccttcc ggtcgctgat gcgcaggaac
     1981 cacgagctct tcgcgttctg ctggtagtcg agtcgcaggt cgcccttatc ggagttatcg
     2041 gtgaaaggca cctccaccga atagtcgttc gagttcagcc cggtcgtcgc cagtcccgcc
     2101 tgcggcagcg gcagtttctt gaagtaattc actacctgcg cgttcagcgg atcgatcgcc
     2161 gtgttcggaa ttgccgttcc cgccggatac accgtgcccg tcagcggatt ccgcaccggt
     2221 accaccagga ttccgttcag ctcgttctgc gtgggcagcg tcagcacgct cagcggtgtc
     2281 agcgtctgcc gaaaaccttc gtagtcgagg aagtaaaaca atctgtcccg caggatcggg
     2341 ccgccgaagt tcataccgaa ctgattgcga ttgaacgcag gcttatggaa cggcgtcacc
     2401 ttccccgtgt tgctgaccac gttcggtttg aagaatcccg ctgcattcag atccgtgttg
     2461 cgcaggaatt catacagtgt gaagtgatac gcattcgtac cgctcgctga cgccacattg
     2521 accgtcgctg ccgaaccgcg cccgtactcc gcgctctcat tgttcgtgac aacgctgaac
     2581 tgcgctaccg agtccggcgg aatcgcgatg atctgattgt cgaacccctg attgctctcc
     2641 ccgtacgcgt tgttgtccat cccgtccagc agaaaattgt tgaacatgct ccgctggccg
     2701 ttcacgttgt atgaaccggc gcgcaccagg ctcgagatgg aactcgtcgt cgcctgagaa
     2761 ggcgcctgcc gcgcccccgt caccacgccc agcagatccg aataattccg gcttaccagc
     2821 ggcagcgctt cggtctgata gttcgtgatc gtctgcccgc gctggctcga atccgtttcc
     2881 agctgcagcg ccacgtcgct cacttccacc gtcgtctcgg tttgccccgg caccagccgc
     2941 aggtcaatgc gctgccggct tcctaccgaa atcgtgatgt tgcgggcgat ggccggagca
     3001 aatccctgct gcgacgcgtt gatcgtgtaa acacccgtgc gcagggaagg gaactcgtag
     3061 tcgccctgat cgttggacgt ggttttctct gtgatcccgg ttgccgtgtt cgtaacgctc
     3121 accgcggttc ggggaaccac cgctccgctg gagtcgcgga ccgttcccac caggctgcca
     3181 ttttcatatt gcccgtgcgc cagaggcgag cccgcgaaga acaagcctgc cagaaagagg
     3241 actacccaat aaactcgatt tcttccgttc atgctcctga tcctcggggg cgtagaagaa
     3301 tgccccgtta atacttagca aatgagagat gaatctgtca taaccgatgt gtaaaatcgt
     3361 caccgacatg gattccgggc cttcccccac tccccgccgt atcctcgccg tggatgacga
     3421 tccggtgagt ctggcagtga ctgccgtcct gctggaagcc gaaggctgtg tcgtccttca
     3481 ggccgaaagc ggcgtccagg ccctcgatct cgcccgttcc gaccctcccg actgcatcct
     3541 cgccgacctc cgcatgcccg gtctgaacgg ccctgccctc gcccgctccc tgcgcaaagc
     3601 cgcgccccgc gctcttctcc ttgctatgag cgccacccct ccgccggagc tcgacggcta
     3661 cgacgccgtc cttaaaaagc cgctctctcc ggaaagcctg cgctctgcat ttgcccgccg
     3721 ccccgtcccc gcgccccccg ctcccaaatc gcccaaagaa gacgcggtcc tcgacgccga
     3781 catcttcgag cgcctccgcc gcgccatcac ccccgagggc ctcgaagaag tctttaccgt
     3841 cttcctcagc gacacccatc agcgcatcgc cgccatgcgc aaagccgacg cttcgacgat
     3901 ccgcaaagag gcccatacaa tcaaaggtgg tgcctccatg gtcggcgctc tccagatctc
     3961 ccgcgccgca gccgtgctcg aatcgggtgt tgacgatcct gatgatcggt tgcgcaaact
     4021 cgacgagatg gaggctcatt gccgtttcgc cgcaattatc ctgaaacaga ggcttaaagc
     4081 atgaactcgc ccacaaagtc tgctcggccc gccgttcccg cccggccgtc aaaatccgcg
     4141 ccccccatcc gcatcctcat cgccgacgac catcccgtca tccgcatcgg cgtccgcaac
     4201 atgctccagg ccgagggcgg gctcgatgtc gtcggcgagt gctccgatgg cgatgaggcc
     4261 atcacccaaa cgctcgaact gctccccgac atcctcctcc tcgacgtcta catgccccgc
     4321 ctgcccggca tcgaggccat gcgcgccatc atgaacggct cgccgtcggt caagatcatc
     4381 ctcctcacca gcaccatcag cacccagcag atcatcgaag ccctgcagat tggcgcccgc
     4441 ggcatcgtca tcaaagacgt cgtcgcagaa cacatcaccg ccgccattcg cgctgtcgcc
     4501 ggcggcgact actggatcgg cggcaagcgc gtcgtcaatc tggtcggcgc tctgcacgac
     4561 ctcatgcagc aggccgctgt tcccgaccgc aagaccttcg gcctcacccc gcgcgaaatg
     4621 gaagtcgtcg gctgcatcgt cgaaggctgc tccaaccgcg acatcgccaa acagttcggg
     4681 ctcagtgaag aaaccgtaaa gcgccatctc tccaacatct tcgacaaaac cggcgtctcc
     4741 acccgtctcg agctggccat gttcgccatc gcccaccacc tggtcgccac cgagcactag
     4801 accagcgcag ttcccaagtc aaaacgccct aaagataggc atctcgtccc gagatgccct
     4861 ttctttgttc tggcgattcg tcggctcccg atgatacggt ccctctgaac ccccgcgcgc
     4921 gccggcatcc tttctcaccg tgctgtggcc atctcaacat tcccaggatc tccgatgatg
     4981 caagcttcgt ccgtcctgct ctgttgtgtt ttcctcgcct ccctcacacc ggctgcccac
     5041 acccaaaacc cgccgtctgc ctcctctgtc cagctcaccc cgtacaccac tcccgatcaa
     5101 agcgcctccg ccggcgttcc ctctggctgg aaagccaccg gctcccaaac caccatcact
     5161 ctcaccggtc cacaaaacga atccatcact ctgggacgcg tctacatcgc ccgtaacgcg
     5221 cccttccagg caggacaaag acccggcggc ggggctgacc tcgccatgcc ctataacgcc
     5281 cctctcaccc aaaaactcat tgccgtctac cagcaggcct acgccctcgc cggcaagccc
     5341 gtcccgcaga tcgccttcac ctccaccacc ccgctccagg tcccctccat cctcggccag
     5401 tgcggccgct tcgtcgccaa cctcaccgga gccggtgacc ccgcaaagat catgggcatc
     5461 ttctgctcct ttcccctcga ctccgccggc gcgttcaaaa atttcatcct cgtcgcgcag
     5521 gctcccgcct ccaccgctgc gcaggaagcg cccctcgccc aggccgtctt ttccagctat
     5581 cggatccctc aggccatgct ggccaggaag ctcgcgcccg tcaccgcgcc gcctccaccc
     5641 agcagcggac ccggcgccgg tatgagcagc accttgtggg gcatgcgcca ggccgacatc
     5701 atggccacct gtatggacga gggcgtcatc cgcgagtatg gcccccgcca gcttccccgc
     5761 gaatgcggcg gcctcgctcc caacccctaa gcgcaccacg cgccccctcc acctgtaccc
     5821 aggcgctctc tgccctgtac cctgtaaatg caagggggct gacgaaggaa tgccgtccga
     5881 acaagtcgct gcagtggaag ccggccacaa tactacctgg tcccgcaaca acccttactc
     5941 cgccacggtc ctcgaaaacc ggctccttac cggctccggc tcggaaaaag aaacccgcca
     6001 tatcgagttc gacctcgccg actccggcat tacctatctc cccggcgact ccgccggcat
     6061 cctccccatc aacgcccagg acgccgtcga cctcgtcctc gaacgcctca ccctcaccgg
     6121 ccaggagccc gtcaccgact tctacggcaa gcccctcgac ctccgccagg ctctcacctc
     6181 ctggctcatg atcggcaaag tctccggctc caccctcaaa gcctgggcca aacacaccgg
     6241 caacgccgag ctctccgaca tcgccgccaa ccgcgaaaag ctcgacgcct acctctgggg
     6301 ccgcgagttc ctcgacctcc tcgaacacgc acccggcaaa atcgaagacc cccagcagct
     6361 cttcaaaatc ctcccgcgtc tcgccccgcg cctctattcc atctcatcga gccaggcgct
     6421 tctcggcacc cagatcgatc tctgcatccg cgtcgtcaaa tacaacaccc atggacgcga
     6481 ccgccacggc gtctgctcca cccacgtcgg cgagcgcacc cccaaaggcg aaaagctccc
     6541 catcttcatc cattccaatc agctcttccg cctgcccgcc gaccccgacg cgcgcgtcat
     6601 catggtcggc cctggcaccg gcgtcgctcc cttccgcgcc ttcctccagc acaagcaggc
     6661 cggacagggc cgctggccca tgtggctcgt cttcggcgac cagcgcgc
//
LOCUS       DASZYN010000495         1727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_791450, whole genome shotgun sequence.
ACCESSION   DASZYN010000495 DASZYN010000000
VERSION     DASZYN010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791450"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..939)
                     /locus_tag="VHE33_12775"
     CDS             complement(22..939)
                     /locus_tag="VHE33_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98372.1"
                     /translation="MAIETVSFAGLPNCLKISNGATDLIVTTAVGPRILFYGPTGGRN
                     HLGSFPDSSVKTALGSWKPYGGHRLWVWPELFPATYAPDNSPIEHEVHGDLSVTVHQR
                     ADGAAIEKHIRITLAPSGSNVRLEHTVTSSNLWPIDIAIWAITIVASGVGIVPRVPFQ
                     THDEYPPVTQPLALCAFTDLQDPRFTLGLKYILLRADPARANAQKFGLRNKEGWCAHL
                     LGDELFVKRFAHHNRANYPDYQVNTEVYVEGAFQEVELMGPRQTVWPGESLQHSEEWN
                     LFQGVQVAPGAPDLDELDRALTPLIRSLS"
BASE COUNT          368 a          432 c          610 g          317 t
ORIGIN      
        1 gggtgggtgg gccttgagca gctaagaaag tgaacgaatc agcggcgtca gtgcgcggtc
       61 gagttcatcg agatcgggcg cgccgggggc cacttgcacg ccctgaaaga gattccattc
      121 ttcagagtgc tgcagggatt cgccgggcca gacggtctga cgcgggccca tgagctccac
      181 ttcctggaag gctccctcga catagacttc ggtatttacc tggtagtccg gataattggc
      241 gcgattatgg tgggcaaagc gcttgacgaa gagctcgtcg ccgagcagat gcgcacacca
      301 cccttccttg ttgcggagac caaacttctg cgcgttggcg cgggcgggat cggcgcggag
      361 gaggatgtat ttgaggccga gggtgaagcg gggatcctgg agatcggtga aggcgcagag
      421 ggcgagaggc tgcgtaaccg gcgggtattc atcgtgggtc tggaaaggaa cgcgcgggac
      481 aatgccgacg ccggaggcga cgatcgtgat ggcccagatg gcaatgtcaa tgggccagag
      541 gttgctgctg gtcacggtgt gctcgagacg aacgttggat ccggaaggcg cgagggtgat
      601 gcggatgtgt ttttcgatgg ccgcgccgtc ggcccgctgg tgcacggtca cgctgagatc
      661 gccatggact tcgtgctcga tgggtgagtt gtccggtgcg taggtggcag gaaacagctc
      721 aggccacacc cacaggcgat gaccgccata gggtttccag gaaccgagag cggtcttgac
      781 ggagctgtcg gggaagctgc cgaggtgatt gcggccaccg gtggggccat agaagagaat
      841 gcgcggaccg acggcggtgg tgacaatgag gtcggtcgcg ccgttggaaa ttttcaggca
      901 gttcggcagt cctgcaaagg atacggtttc gatcgccatc gagatgcgcc tcggttccga
      961 gtagggaata aggaagcacc attgtagagg atcggtgtgt cctgctccgg gacggattgg
     1021 acccgccggc cggagccatg aaagcgattg gcgctcgctg acatgaggat gcgggaggtt
     1081 gcgatgctgc gggtcgttta tgaacaggcg tccagagctt cgccggctct tgcgcttttg
     1141 tctgagctga acatcgaggc acgatcgccc cagccgcggt gggaggaact gacggcgtcg
     1201 gacggcgatc cacacacagg cgcgagcata tatttgtcac tctttttgtt acatattcaa
     1261 agtgaaccac aacggaaagc ccaattccgg gctgtgccga gggcttgcac agggggtggt
     1321 gcaggccgca attttcttcc agcatttgca cataaatacc cggatttgca cagaaataaa
     1381 ggacttgcga gaccacgatt ctgtggcatg attctttcaa cggaggagca atcctcccga
     1441 agaagagagc agaacgaagc ggtcaggagc agcggttaac gcggctcagg gggctcacgg
     1501 gaaaccgggg gtctcagcag gaaggccggg caaccggacg cctgcggatg gcgacgaaga
     1561 cgctcacacg tcagatgggt tgacggaagc atcgggaagc gggggcgcaa gctctttggt
     1621 ccaggtgaaa ccggcagcaa cagggcgtga tcaggtagcc gaagccagat cttcgataaa
     1681 tcggaagaag gggcagggac ccgcaagggg acctgcccct tcgactt
//
LOCUS       DASZYN010000496         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_600060, whole genome shotgun sequence.
ACCESSION   DASZYN010000496 DASZYN010000000
VERSION     DASZYN010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600060"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VHE33_12780"
     CDS             complement(<1..767)
                     /locus_tag="VHE33_12780"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT98373.1"
                     /translation="MPSRKAAFSVRRSAFTNTILSFAVPAIILVGGLTALKARPAAGN
                     SNPAEAARLNSIGVALMNQQLTEKAAAELDQARAADPKSPIPVLNRGIALLYMQKLPE
                     AEAALRQAGTMAPDNPRAWYNLGLTEMDEANQKGAIEDMRKVLAIDPKDADAHYFVGS
                     LEMSLGDANAAIAEFQTALKLNPLHCSAEFGLARALQRAGRVEEAHEHLKIFQHFTQA
                     KIAAPLSVGYGERGHYSKMEEMQLPVEAAGPMIPVKF"
     gene            complement(870..1910)
                     /gene="moaA"
                     /locus_tag="VHE33_12785"
     CDS             complement(870..1910)
                     /gene="moaA"
                     /locus_tag="VHE33_12785"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HVT98374.1"
                     /translation="MASALDSIPTVAKGQAERLIDKHGRAITDLRISITDRCNYKCVY
                     CRTGNEGAVYAELPIADYLRMVRVFVGLGIEKIRLTGGEPLLRRGLLEMIRELSQLRT
                     LDGAPLDLALTTNGHLLEELAEPLRDAGLSRITVSMDAVDAEKFARITRVPGSYERVL
                     AGVRAAKKAGLAPVKVNCVLLRGFNEDQIVRFAQFSRDEGVIVRFIEFMPLEEDRLWT
                     PESVVSLKEIVAALESYRPVVRMAPNVASETAVRYTFDDGVGEVGIIAPVTQAFCGHC
                     SRVRLTSDGKIRTCLFSQSDHDLYGVMQRGGSDDDMVAFIRQTIGKKEARHHIGEPDF
                     LKPSRSMVHIGG"
BASE COUNT          349 a          748 c          523 g          386 t
ORIGIN      
        1 tcaaacttca ccgggatcat cggccccgcc gcctctaccg gcagctgcat ctcctccatc
       61 ttcgagtagt gcccgcgttc cccatacccc acgctgagcg gcgcagcgat cttcgcctgc
      121 gtgaagtgct ggaaaatctt caggtgctcg tgcgcctcct ccacccgccc cgcgcgctgc
      181 agcgcccgcg ccagcccaaa ctccgccgaa caatgcagcg gattcagctt cagcgccgtc
      241 tggaactccg caatcgccgc attcgcatcg cccaggctca tctccagcga tccgacgaag
      301 taatgcgcat cggcgtcctt cggatcgatg gcgaggacct tccgcatgtc ctcgatcgcg
      361 cccttctgat tggcctcgtc catctccgtc aggcccaggt tgtaccaggc tcgcggattg
      421 tccggcgcca tcgtgcccgc ctggcgcagc gccgcctccg cctccggcag tttttgcatg
      481 tacaagagag cgatcccgcg gttcagcact ggaatgggcg acttcgggtc cgcagcacgc
      541 gcctgatcca gttccgctgc tgccttctcg gtcagctgct ggttcatcag cgccacaccg
      601 atactgttca gccgcgccgc ctctgctgga ttggaattgc ccgccgccgg ccgcgccttc
      661 agcgcagtca gaccgccaac cagaatgatg gctggcaccg caaagctgag aattgtattc
      721 gtgaacgctg aacgccgaac gctgaacgct gcttttctgg agggcatctt cgccgtccat
      781 tataaggatc ggcaaatact gctcgcatcc ggtgctttct gaacgccgaa cgctgaacgc
      841 cggaggatga tccggcgctt cgcggcggat catcctccga tatgcaccat gctgcgcgag
      901 ggcttcagga aatccggctc tccgatatgg tgccgcgctt ctttcttccc gatcgtttgc
      961 cggatgaacg cgaccatgtc atcgtcgctg ccgccgcgct gcatcacgcc atacaggtcg
     1021 tgatcgctct gcgagaacag gcaggtgcga atctttccgt ccgacgtcag ccgcacccgg
     1081 ctgcagtgcc cacaaaacgc ctgcgtcacc ggcgcgatga tccctacctc ccccacgccg
     1141 tcgtcgaatg tgtaccgcac cgccgtctcg ctcgcaacgt ttggagccat ccgcaccact
     1201 ggtcggtaac tctcgagtgc cgcaacaatc tccttcagcg acacgaccga ttccggcgtc
     1261 cacagccggt cctcttccag cggcatgaac tcgatgaacc gcacgatcac gccctcatcg
     1321 cgcgaaaact gcgcaaaccg cacgatctgg tcctcattga atccgcgcag caggacgcag
     1381 ttcaccttca ctggcgccaa accggccttc ttcgcggccc gcacccccgc cagcacacgc
     1441 tcgtagctgc ctggcacacg cgtgatacgc gcaaacttct ccgcatccac cgcgtccatg
     1501 ctcaccgtga tccggctcag ccccgcatcg cgcagcggtt ccgccagttc ctccagcaaa
     1561 tgcccgttgg tggtcagcgc cagatccagg ggcgctccgt ccagggtccg cagctgactc
     1621 agctcgcgga tcatctccag caacccccgg cgcagcagcg gttcgccgcc cgtcagccgg
     1681 atcttctcaa tccccaaccc gacaaacacc cgcaccatgc gcaggtaatc cgcgatcggc
     1741 aactccgcat acaccgcgcc ttcgttgccc gtgcggcaat agacacactt gtaattgcac
     1801 cgatctgtaa tcgagatgcg caggtcggtg atcgcccgcc cgtgcttgtc gatcaggcgc
     1861 tcggcctggc cctttgccac agtcgggatg gaatcgagcg cactcgccat gagcttctac
     1921 tttattcgat gcgccacaac cccgtcagct tccgcccggg cttcaacccg ccggttgtcc
     1981 atctccctcg ccggcagacg gcctcc
//
LOCUS       DASZYN010000497         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1242915, whole genome shotgun sequence.
ACCESSION   DASZYN010000497 DASZYN010000000
VERSION     DASZYN010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242915"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1774)
                     /locus_tag="VHE33_12790"
     CDS             complement(125..1774)
                     /locus_tag="VHE33_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98375.1"
                     /translation="MINPNDPPRGSMYLYDDIQPATVDGQYKLTVSTDISWDSNSESA
                     PIDRYLNIVGPRFSLDPTLVANVYPPRNGQGAFQDAIPQIVLSRRTLPWERDAALPAP
                     VVDPSLPAQNGQVPWVALLLFQDGEPYTLLQNQHLVDVVGTGVFHKLGSPANVLCDAV
                     ELDRSLLTDILPTSHELRLLSHVRQVNVEDRELSAGSSDGFFSVVMSNRLPSPETKYR
                     ACLVSLEQRADLLQPLQVPPVLADAAPGNAGGNIAAREIAIVVRGPGSLLHPKVRLVL
                     LHTWQFTCTGPGPFRKLMQALDVGMIGKVATPGQPALTDTGHLRLSIQDRAGTTEVGW
                     YRGPLVPWQLTRDPLGPYHSADQCRRATVETGAEDVSYAAAFEVGRQLAAADGQLARE
                     LMRWRTEAYRQANRADNITQKIAPALAVDLPATLAEQLHTSLPPLVSVSATEAIAKGA
                     PGVADRYRIDHASQTVGMDPQVLSEVWSLASTAEAQILLGSDPSTLGATVAEAPQTVR
                     SNTTLEQVAADTGSLSRLSQARNRILTNATVVAQNNTGGKS"
     gene            complement(1771..>2187)
                     /locus_tag="VHE33_12795"
     CDS             complement(1771..>2187)
                     /locus_tag="VHE33_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98376.1"
                     /translation="ISDALIEQSGAETWLPTAVSVIMILLDRADANAAESGDIGISCD
                     GATLATPPVVGSGGLRRALLYDVVEHDPKASHISIAVASQAGWSLAGVIGLPGRAADW
                     AARLHGSVPPSLVSSGPLTASGQILVQITKSAGGVA"
BASE COUNT          435 a          670 c          695 g          387 t
ORIGIN      
        1 cgcaggtgag cgaggaacga ctccatgtag ggcggcattt cggcttcagg aaccggagcc
       61 gccacctgca ggggattcgc catgcgcgcc cgcagcgcat ccacgccggt aatccacgct
      121 ggcctcatga tttgcctccc gtattgttct gcgccacaac ggttgcgttc gtaagaatgc
      181 ggttcctcgc ctgcgaaagc cggctcagcg atccggtatc cgcagcaact tgctccagag
      241 tggtgttgga acgcaccgtt tgaggtgcct cggcaaccgt cgcgcccagc gtcgagggat
      301 cgctgcccag cagaatctgt gcctctgctg tggaggcaag actccagacc tcgctcaaca
      361 cctggggatc catgccgacc gtctgcgaag catgatcgat ccgatagcgg tccgcgacac
      421 ccggtgcgcc tttggcgatg gcttcggtcg ccgagacgga taccagcggc ggaagcgacg
      481 tgtgcaactg ctctgcgagc gtggccggaa gatcgaccgc aagcgcggga gcgatcttct
      541 gcgtgatatt gtccgcgcgg ttggcttggc gataggcctc cgtccgccac cgcatcagtt
      601 cacgggccag ctgtccatcc gccgccgcca gttgacggcc aacctcgaac gcggctgcat
      661 acgaaacatc ttcggcgccg gtttccaccg tggcccggcg gcactggtcg gcactgtgat
      721 acggacccag cgggtcccgc gtaagttgcc acggcacaag cgggccccgg taccatccga
      781 cctcggtggt gccggctcga tcctgaatcg aaaggcgcaa gtgcccagta tcagtgagtg
      841 caggctgacc cggcgttgcc acctttccga tcatgccgac atcgagcgcc tgcatcagct
      901 tcctgaacgg gccagggccc gtgcaggtga attgccaggt gtgcagcaga acaagccgga
      961 ctttgggatg gaggagactg ccaggacctc ggaccacgat cgcgatttcc cgcgctgcga
     1021 tgttgccgcc tgcattgcca ggcgctgcgt ccgcaagcac gggaggaacc tgcagtggct
     1081 gcagcagatc ggcgcgctgt tccagagata cgaggcaagc ccgatacttc gtttcgggcg
     1141 agggaagacg gttggacatg acaaccgaaa agaaaccgtc gctggagcct gcactcagtt
     1201 cgcgatcctc cacgttcacc tggcgaacat gcgaaagaag gcgaagctca tggcttgtgg
     1261 gaagaatgtc cgttaacaag ctgcgatcga gttcgacggc gtcgcacaga acattggccg
     1321 gcgagccgag cttgtgaaac acgccagtac caaccacatc gaccagatgt tggttttgga
     1381 gaagtgtgta gggttctcca tcctgaaaga gaagcagcgc cacccaggga acctgaccat
     1441 tctgcgcagg cagcgacgga tcgaccacag gggccggcaa cgcagcgtcg cgttcccagg
     1501 gcagcgtgcg gcgcgacagc acgatctgag gaatggcatc ctgaaacgct ccctggccgt
     1561 tgcgcggtgg atagacattc gccaccagcg tcgggtcgag cgagaaccgc gggccaacga
     1621 tattcaggta gcggtcgatc ggcgctgatt cggagttgct gtcccatgag atatcggttg
     1681 aaaccgtgag cttgtactgc ccatccacgg tggctggctg aatatcgtcg taaaggtaca
     1741 tcgatccgcg ggggggatcg ttgggattga tcacgcgaca cctccggcag attttgtgat
     1801 ctgaaccagg atctggcccg acgcggtaag cggaccgctg gagaccaggc tgggcggaac
     1861 ggaaccatgc aaacgcgcgg cccagtctgc ggcgcgtccg ggcaggccga tgactccggc
     1921 gagcgaccat ccggcctgac tcgcgaccgc gatagaaata tggctggctt tcggatcgtg
     1981 ttcgaccacg tcgtaaagca gtgcgcggcg aagtcctccg ctgccgacaa ccggaggcgt
     2041 tgccagagta gccccgtcgc aggaaatccc gatatcgccg ctctccgcag cgttcgcatc
     2101 ggcgcgatcc agcagaatca tgatgacgga gaccgccgtt ggaagccatg tttccgcgcc
     2161 gctctgctca atgagcgcat cggaaat
//
LOCUS       DASZYN010000498        11015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_600189, whole genome shotgun sequence.
ACCESSION   DASZYN010000498 DASZYN010000000
VERSION     DASZYN010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 11015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11015
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600189"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VHE33_12800"
     CDS             complement(<1..588)
                     /locus_tag="VHE33_12800"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HVT98377.1"
                     /translation="MTDTNYDKAGAAAEFVLSRTELRPRIGIVLGSGLGAFAEQLTDR
                     AAVPFAEIPHFPQSTVPGHSGKLVIGKVAGVPVAVMQGRVHAYEGYNSDEVVFPIRVL
                     AAMGINQLVLTNAAGGINPAFQRGQLVLIADHINLSGRNPIAGPADDRLGPRFFDMSE
                     AYSGRLRTVAHEAGHEEGYRLDEGVYLSVLGPSFET"
     gene            complement(591..1850)
                     /locus_tag="VHE33_12805"
     CDS             complement(591..1850)
                     /locus_tag="VHE33_12805"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HVT98378.1"
                     /translation="MGRFTGVLGLLTMLLLAWIFSTNRRAIRWRTVIWGLGLQILFAV
                     CVVEWTVGQRVMHAAGGAVNKLLAYAFDGSTFVFGDLGAQHSKIGGAFGTSFFAFQVL
                     PTIIFISAFFAVLYYLGIMQLIIRGFAKAMLWTMKVSGAESLNVAASIFMGQTEAPLT
                     IRPFLPGATRSELMTIMTSGMAHVSGGIMAAYILYGISASSLLAAVIMTAPGTILISK
                     MLVPETEKPATEGVVHIPENEESRNENLIGSIARGTIDGGRLAFNVGIMLISFLALIA
                     LLNGILGGLHNWLGFHHIPFPHSLDAILGFFFAPVAWLIGIPWHDAPTVGNLLGTRMV
                     LNELVAYTYLGSLAKTGALAARSVTIATFALCGFANLSSIGIQIGGIGALAPNRRNEL
                     AKLGFRAMLAGTMANLMSASIVGILMK"
     gene            complement(1878..3242)
                     /locus_tag="VHE33_12810"
     CDS             complement(1878..3242)
                     /locus_tag="VHE33_12810"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HVT98379.1"
                     /translation="MTALHAVDLIRKKRDGEELHAEEIRFLVEGAAQETIPEEQLAAW
                     LMAVFLRGLTLAELDTLTTAMRFSGEVLDHAGLGRSTVDKHSTGGVGDKTSFLVAPIA
                     AAAGLADPMISGRALGHTGGTLDKLESIPGYRTGLSLAEMRTVLEKCGASIVGQTRNL
                     VPADRKLYSLRDRTATVENPYLICASIMSKKLAAGLDALVIDVKTGSGAFLRDEDQSR
                     YLAALMVETGERAGTRTVTVLTDMSQPLGRMAGNWMEIAEAMELLQGERNPLSEDLRE
                     VSLILAGWMIYLGGKARSAQQGREVAEQKLVDGSALGIFREMVEAQGGELKAIDAGME
                     FHQPRFRREFRAARNGYFVAADCTKVGWAVQRLGAGREKAGEPVEAHAGLEMHVKLGD
                     RVEAGQVLATLFAQDEARFAEPEHLLSEALTLADAPLAPPPLVRDIISAENKDSYLQA
                     GHRP"
     gene            complement(3239..3661)
                     /locus_tag="VHE33_12815"
     CDS             complement(3239..3661)
                     /locus_tag="VHE33_12815"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HVT98380.1"
                     /translation="MLDIDEQTLEQLRAAAVKAAGNAYAPYSRFRVGAALLFDDGSIA
                     TGCNVENLSYGLTSCAERNALFRAISEQGPARRVRAVAVANRDGAPCSPCGACRQVMS
                     EFVTPDAVVCFSDRNGPETRRFAELFPFAFAFDPETEA"
     gene            3738..6065
                     /locus_tag="VHE33_12820"
     CDS             3738..6065
                     /locus_tag="VHE33_12820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HVT98381.1"
                     /translation="MDDLTREFLLESQEGLDRMERALTDLETRPGDTELLAEIFRSVH
                     TIKGTTGFLGFSRLEALSHAGENLLGLLRDGKLLATPEIVSGLLTLMDMLRGILRSIE
                     TTGAEGESQAQDTAMIERLEKLQRGDTSAAFAEPILTAAAVAGTARPVRPHKPRVPRK
                     RASRLLPETVQDPPPPNTSEEAGPLSKVEDRSRAPKLEPVPGSSLPARSASEPVPEVQ
                     SAASPGTADSTLRVDVELLNRMMNLVGELVLTRNQILQTTSNDPGFALLGRRLDMVTA
                     DLRESVMKARMQPVSHVFSRFPRLVRDLALGLNKKVRLEMEGQETELDKSLLEAIRDP
                     LTHSIRNAIDHGIESPAERAAQGKPAEGVVRLRAYHEGSHVVVEVHDDGAGMSIDKIC
                     RKAIERGLVTAQRAEQMNEREILQLIFLPGFSTAETVTSVSGRGVGMDVVRTNVEKIG
                     GKVDVESRAGQGSILRLRIPLTLAIIPALIVRSRGQNFAVPQTGLTELVHLSAGESDS
                     RIERLENASLYRLRGHLLPLVFLDRLLQQPARASGTPCDIAILNADGRRYGLVVDGLA
                     DPEEIVVKPLAAVLREIGFYAGATILGNGEMALILNPGAIAQSANVGLSSETEEGGGE
                     ETNAGRQDYLVVEAGARRAALPLDTVVRIERIPRSRIERAGSRPVLRFEGSLLALDDA
                     AADFIDAGEDPDAQMTVVVCRDGRRQVGMTVSQVLDVTGGSQLQEAGSGLAAAGITLL
                     HEKVTGIVSLSAIPPLENVAEDGLAGFAISQEESR"
     gene            6062..6520
                     /locus_tag="VHE33_12825"
     CDS             6062..6520
                     /locus_tag="VHE33_12825"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HVT98382.1"
                     /translation="MSEASGQKTEEVCSVVVGDTLYGIPIAHVLEILGKPGTQPVPLA
                     PYFVGGLVHYRGEVLTAVSLRRLLGMEPAGTPEDVLVFESGDGLFGLFVDSVGEVLHV
                     SETDHEPNPATLEARRQALFDGAWKLRDRLLVSLRPDRLDPMRLAGAFPA"
     gene            6548..6919
                     /locus_tag="VHE33_12830"
     CDS             6548..6919
                     /locus_tag="VHE33_12830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT98383.1"
                     /translation="MRALVVDDSRFIRNYLHSLFTEHGVECVEAVDGKQGLACLREAG
                     PFSVALVDWNMPVMSGYEMLQAARADHAFDGTRIVMVTTEAENDHIEAALLAGADEYI
                     VKPFDEGGLFDKLRMAGVEEL"
     gene            6916..8016
                     /locus_tag="VHE33_12835"
     CDS             6916..8016
                     /locus_tag="VHE33_12835"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HVT98384.1"
                     /translation="MMPGAEIGARRIRVLHIDDSAVMRSLLRMVLEPQASIEIAATAS
                     SGQEGLAAFERIHPDLVLLDIEMPGMNGLEVLSAIRRHDRRVPVIMCSTLTSRGARIT
                     IEALARGATDYVTKPGAQHGVREAVETLSRELLPKILALFPPASHRTAPPLTDVSCPV
                     PPPAAVHRPKIVVIGVSTGGPAALELLLPKIPADFALPILIVQHMPRLFTGLLAERLN
                     SVCSLRVREAAPDTQAEAGVIDIARGDWHLEVHRDLRLRLNQNPPENFCRPSVDVLFR
                     SAAQVCAGGVLGVILTGMGSDGLIGSRAIRAGGGTVLAQDGATSVVWGMPGAVAQAGL
                     ANKILALDAIPAEILRLTAATPPQARRVEAFA"
     gene            8016..8834
                     /locus_tag="VHE33_12840"
     CDS             8016..8834
                     /locus_tag="VHE33_12840"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HVT98385.1"
                     /translation="MSASPIDYEYLRTVVLRNSGNQVDASRNYLFESRLQGLLRNRGF
                     DSLTQLVSALRSGNGRSLERSVAEAMTINETSFFRDTPTFDYLRETLLPALIESRRDT
                     RTLRFWSAASSSGQEAYSIAMMLREYFPGLAGWRIDILGTDISAEMVERASTGRYQRI
                     EVNRGLPARLLVKYFVRINDEWEIQPDLKRLCRFQQRNLCPLPSLPETFDIIFLRNVM
                     IYFNQDTRREVLLGMHRALAADGILFLGLSEQPRMDSHWQTMLTPKTVWYKPIR"
     gene            8956..>11015
                     /locus_tag="VHE33_12845"
     CDS             8956..>11015
                     /locus_tag="VHE33_12845"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVT98386.1"
                     /translation="MDPLSLFHPITRSWFRSTLGEPTSPQRLGWPAIARRESTLILAP
                     TGSGKTLAAFLSCLDRLMLREPASETRATEPGCRVVYISPLKALAVDVERNLQTPLQQ
                     IAPLARRAGVPFREPQIAIRTGDTPQIERARFRRHPADILITTPESLYLLLTSDSATA
                     LRSVDTVIVDEIHALVPSKRGAHLVLTLERLQAICTQPLQRIGLSATQRPLEEVARFL
                     GGSQTSEDPSEVTARPVTIINASAPKQLQLRVEVPVEDMKAPESSPAFGQDSPVKRAS
                     VWTTIPPRLLAIIRRRRATLIFVNNRRTAERLAGLVNDLAQEPLARAHHGSLAAAQRA
                     EIEEQLKKGGIRAVVATSSLELGIDMGAIDLVIQIESPPSVAAGMQRIGRAGHKVGVV
                     SEGIIFPKYRADLIACSAVTRAMHEGHVEATSMLRNPLDVLAQQIVATVAQPPALAEP
                     RLKGHAPREVSISADSVYDLVRRAAPFTTLTRAAFESVLDLLSGKYPSEEFSELRPRI
                     NWDREANLLAPRAGSKSLAILNAGTIPDRGLYGVFLSGEHKKPVRVGELDEEMVFESR
                     VGEVFALGASTWRIDEISHDRVLVSPAPGQPGKLPFWHGDQAGRPLEFGRRIGALVRE
                     IRALPPNAAISRLVREHDLDSQAAENLLQFLSDQQNVTATVPDDRCIVIERIRDELGD
                     WRVCV"
BASE COUNT         2004 a         3554 c         3405 g         2052 t
ORIGIN      
        1 cgtctcgaag cttgggccga gcacgctcag atacacgccc tcgtcgaggc gatagccctc
       61 ctcatgcccc gcctcgtgcg ccaccgtccg tagccgcccg gagtacgcct cgctcatgtc
      121 gaagaaccgc ggtcccaagc ggtcatctgc gggaccggca atgggattcc gtccggacag
      181 gttgatgtgg tccgcgatca ggaccaattg tccccgctga aatgcgggat tgatgccgcc
      241 cgcggcattc gtcagcacca gttggtttat gcccatcgcc gcgagcactc ggatgggaaa
      301 gacgacttcg tccgagttgt atccctcata tgcatggacg cgtccctgca tcacggccac
      361 cggtacaccg gcgactttgc cgatgacgag cttgccactg tgtccgggca cggtcgactg
      421 cggaaagtgc gggatctctg caaagggaac ggcggccctg tccgtcagct gctcggcaaa
      481 tgcgcccagg ccggagccca gaacgatccc gattcgcggc cgcagctccg tccgcgacag
      541 cacgaactcc gccgccgcac ctgccttgtc ataattcgta tccgtcatag ttacttcatc
      601 agaattccca cgatcgaagc cgacatcagg ttggccatgg tgccggccag catcgcgcga
      661 aatcccagtt tggccagctc gttgcggcgg ttcggggcca gcgccccgat gccgccgatc
      721 tggatgccga tcgagctcag gttggcaaat ccgcacaacg caaaggtcgc aatcgtcacg
      781 gagcgcgccg ccagggcccc cgtcttcgcg aggctgccga gataggtgta cgccaccagc
      841 tcattcagaa ccatgcgcgt gcccaacagg ttgccgacgg tcggcgcgtc atgccatgga
      901 atcccaatca gccacgccac cggcgcaaag aagaacccga ggatcgcgtc aagcgagtgc
      961 ggaaatggga tgtggtgaaa cccgagccag ttgtgcaggc cgcccaaaat cccgttcagc
     1021 agcgcaatca gcgccagaaa cgagatcagc ataatgccga cgttgaaggc caggcggccg
     1081 ccgtcgatcg ttccgcgcgc aatggatccg atcagattct cgttccggct ctcctcattt
     1141 tccgggatgt ggaccacgcc ttccgtcgcc ggcttctccg tctccggtac gagcatcttc
     1201 gagatcagga tcgttccggg cgccgtcatg atcaccgcgg cgagcagact gcttgccgag
     1261 atgccgtaca ggatgtacgc cgccatgatt ccgccggaca catgcgccat gccgctggtc
     1321 atgatcgtca tcagctcgga gcgtgtggct ccaggcagga acggccggat ggtcagcggc
     1381 gcctcggtct gtcccatgaa gatgctggcc gcgacattca ggctttcggc gccggacact
     1441 ttcatcgtcc acagcatggc tttggcgaag ccgcgaatga tgagctgcat gatgccgagg
     1501 tagtagagca cagcaaagaa ggccgagatg aagatgatcg tgggcaggac ctggaaggcg
     1561 aagaagctgg tgccgaaggc cccgccgatc ttcgagtgct gcgcgcccag atcgccaaag
     1621 acaaacgtcg acccatcgaa cgcgtaggcg agcagtttgt tcactgcgcc gcccgcggca
     1681 tgcatcactc gctgccctac cgtccactcc accacacaaa cagcaaagag gatttgcagc
     1741 cccagacccc agataaccgt gcgccatcgg atggctctgc gattcgtcga gaagatccag
     1801 gccaacagca gcatcgtcag cagacccagc acacccgtga aacgtcccaa tgcattctcc
     1861 tgaaggtctt ttcgattcta tgggcgatgt ccggcctgga gataagaatc tttattctcg
     1921 gcggagatga tgtcgcgcac cagcggcgga ggagcgaggg gtgcgtcggc aagggtgagc
     1981 gcttccgaga gcaggtgctc cggctcagcg aatcgcgcct cgtcctgcgc aaagagcgtc
     2041 gccagcactt gtcccgcctc gacgcgatcg ccgagcttca cgtgcatctc gaggccagca
     2101 tgagcttcga ccggctcgcc agccttctca cgtccggcgc cgaggcgctg cacggcccag
     2161 ccgacttttg tgcagtcggc tgcgacaaaa tatccgtttc gagcggcgcg aaactcccgg
     2221 cggaatcggg gctgatggaa ttccattccg gcatcgattg ccttcagttc cccgccctgc
     2281 gcctcgacca tctcgcgaaa aatgccgagc gccgagccat cgaccagctt ctgctcggca
     2341 acttccctcc cttgctgtgc gctgcgcgcc ttgccgccca gataaatcat ccatccggcg
     2401 agaatcagcg acacctctcg caaatcctcg ctcaacggat tgcgctcgcc ctgcaacaac
     2461 tccatggctt cggcgatctc catccagttc cctgccatcc ggcccagcgg ctggctcata
     2521 tcggtcaaca ccgtgacggt cctggttccc gcgcgctcgc cggtctcgac catgagcgcg
     2581 gccagatagc gcgactgatc ctcatcgcgc aggaaagcgc cggaccctgt cttcacatcg
     2641 atcaccaacg catccagccc ggcggcaagc tttttgctca taatcgatgc gcagatcaga
     2701 tacggattct ccacggtcgc cgtccggtcg cgcaggctgt agagcttgcg atcggccggg
     2761 accaggttcc gggtctgccc cacgatcgag gcgccgcatt tctccagcac ggtcctcatc
     2821 tcggccaggg acaggccggt ccggtagccg gggatggact cgagcttgtc cagcgttccc
     2881 ccggtatggc cgagcgcgcg gccactgatc atcggatccg ccagaccggc tgcggccgcg
     2941 atgggagcca ccaggaacga tgtcttgtcg cccacgccgc cggtggagtg cttgtcgacg
     3001 gtgctgcggc cgaggccggc atggtcgagg acctcgccgg aaaagcgcat ggcggtggtc
     3061 agcgtatcca gctcggcgag cgtgaggccg cgaaggaaga ccgccatcag ccaggcggca
     3121 agctgctcct cgggaatggt ctcctgggcc gcgccctcga ccaggaaccg aatttcttct
     3181 gcgtggagtt cctcaccgtc gcgttttttc cggatgaggt cgaccgcatg gagtgccgtc
     3241 atgcctccgt ctccggatcg aatgcaaaag caaacggaaa gagctcagca aagcgccgcg
     3301 tctcgggccc atttcgatcc gagaagcaaa cgaccgcatc gggggtgaca aattcgctca
     3361 tcacctgccg gcaggccccg cagggagaac agggtgcacc gtctcgattg gccaccgcca
     3421 cggctcgaac tcgtcgcgcg gggccctgtt cactgatggc tcgaaacagg gcattccgtt
     3481 cggcgcagct cgtcagaccg taggagaggt tctccacatt gcagccggtg gcgatcgagc
     3541 catcgtcgaa caggagagcg gccccaaccc ggaacctgga atacggggcg taggcattcc
     3601 cggcagcctt caccgccgca gcacggagct gttcaagcgt ttgttcatcg atgtccagca
     3661 tggttctgag gctaactgga tcgcggtccc gcggcaacag gactcaagtt ccgcgttgcg
     3721 cttccgataa acccatcgtg gacgacctca ctcgcgaatt cctgcttgaa agccaggaag
     3781 ggctcgatcg gatggaacgg gctctgaccg atcttgaaac ccgtcccggc gatactgagt
     3841 tgctggccga gattttccgg tcggtgcaca ccatcaaggg caccaccggc ttcctcggct
     3901 tcagccgtct cgaagccctc tcacacgccg gagaaaacct cctcggactg ctgcgtgacg
     3961 gtaagctgct ggcgacgccg gagattgtga gcgggttgct cacgctcatg gatatgctgc
     4021 gcggaatcct ccgctccatc gaaaccacgg gagcagaagg ggaaagccag gcgcaggaca
     4081 cggcgatgat cgagcggctc gaaaaactgc agcgcgggga tacttccgcg gctttcgcgg
     4141 agcccatcct gacggcggcc gcggtggcag gaacggcccg tccggtgcgg ccgcacaagc
     4201 cgcgggtacc gcgcaaacgc gcgtcccgtc tattgcccga gacagttcag gatccgcctc
     4261 ctccaaacac atccgaggaa gcagggccgc tcagcaaggt cgaagacagg tcgagggctc
     4321 cgaaactgga acccgttccc ggctcttcac ttcctgcgcg gtctgcgagc gagccggtac
     4381 ccgaggttca gtcggcggcc tctccgggca ccgctgactc gaccctgcgc gtcgatgtgg
     4441 agctgctgaa ccggatgatg aatctggtcg gggagctcgt gctgacgcgc aaccagattc
     4501 tgcagacgac gagcaacgat cccggcttcg cgctgctcgg ccgccgactc gacatggtaa
     4561 cggccgatct gcgcgagtcg gtgatgaagg cacggatgca gccagtgagc cacgtttttt
     4621 cccgcttccc gcggctggtc cgcgacctgg cactgggact caataaaaag gtccgcctgg
     4681 agatggaagg gcaggagacg gaactggaca agagcctgct cgaagcgatt cgcgatcccc
     4741 tgacgcattc gatccggaat gcgatcgacc acggcatcga atccccggca gagcgtgccg
     4801 ctcagggtaa gccggccgaa ggcgtcgtgc gcctgcgtgc ttaccacgag ggcagccacg
     4861 tcgtcgtcga ggttcacgac gatggtgccg gtatgagcat cgacaaaatc tgccgcaagg
     4921 cgatcgagag gggcctcgtg accgcgcagc gcgccgagca gatgaacgag cgcgagattc
     4981 tgcagctgat cttcctgccc ggcttctcca cggcggagac cgtgaccagc gtctccgggc
     5041 gaggcgtggg gatggatgtg gtgcggacga acgtggaaaa gattggcggc aaggtcgatg
     5101 tggagagccg cgccggccag ggctccattc tccggcttcg catcccgctc acgctggcca
     5161 ttattccagc gctgattgtg cgcagccggg gccagaactt cgccgtgccc cagaccgggc
     5221 tgaccgaact ggttcatctg agcgctggcg agtccgacag ccgcatcgag cggctggaaa
     5281 acgcctcgct ctaccgcctg cgcgggcacc tgcttccgct cgtctttctt gacaggcttc
     5341 tgcagcagcc agcccgggcg agcggtactc cctgcgacat cgccatcctc aatgccgatg
     5401 gacgccgtta cggccttgtt gtcgatgggc tcgccgatcc cgaggagatt gtggtgaagc
     5461 ctctggcggc tgtgctgcgc gagatcggct tctatgcagg ggccaccatt ctcggcaatg
     5521 gggagatggc cctcatcctg aacccaggcg ccatcgccca gagcgcgaac gtcggtcttt
     5581 cttcggaaac tgaagaaggg ggcggcgaag aaacaaacgc cggccgccag gactatctgg
     5641 tcgtggaggc aggcgcccgc cgtgcggccc tgccgctgga caccgtggtt cgcatcgagc
     5701 ggatcccgcg ctcgcgcatc gaacgagccg gctcgcgccc tgtcctgcgc ttcgaaggct
     5761 cactgctggc cctggacgac gcggcagccg atttcatcga cgcaggcgaa gatcccgatg
     5821 ctcagatgac cgtcgtggtc tgtcgcgatg gccgccgcca ggtagggatg acggtctccc
     5881 aagtcctcga tgtcacgggc ggaagccagc ttcaggaggc aggatccggc ctcgcggccg
     5941 cgggcatcac gctgctgcat gaaaaggtaa ccggcattgt cagtctctcc gcgattccgc
     6001 cgctggagaa tgtcgcggag gacggccttg cggggtttgc gatatcccag gaggaatcac
     6061 gatgagcgaa gcaagcggcc agaagaccga ggaggtctgc tcggtggtcg tcggcgatac
     6121 cctttacggg ataccgatcg cccacgtgct tgaaattctt ggaaagccgg ggacccagcc
     6181 ggtccctctg gcgccatact ttgtcggcgg tctggtgcac taccgcggcg aagtcctgac
     6241 ggcggtttcc ctgcgccggc tcctgggaat ggagccggcc gggacgccgg aagacgtgct
     6301 ggtcttcgaa agcggagatg gattgtttgg cctgttcgtc gactcggtgg gggaggtact
     6361 gcacgtgtcg gagacagacc acgagcccaa tccggccacg ctggaggcgc ggcgtcaggc
     6421 gctcttcgat ggagcgtgga agctgaggga tcggcttttg gtcagtctgc gacccgaccg
     6481 tctcgatccg atgcgcctgg ctggagcttt tcccgcctga ggccggtgcc tgcggaaagg
     6541 agaacccatg cgcgctctgg tggtcgacga ttcacggttc attcgcaact atctccactc
     6601 gcttttcacc gagcatggcg ttgagtgcgt cgaggcggtc gacggaaagc agggtctggc
     6661 ttgtctgcgt gaggctggtc cgttctcggt cgcgctggtg gactggaaca tgccggtcat
     6721 gtcgggatac gagatgctgc aggccgcgcg cgccgatcac gcgttcgatg ggaccaggat
     6781 tgtgatggtg acgaccgagg cggaaaacga ccatatcgaa gccgctctgc tggcaggcgc
     6841 cgacgaatac atcgtcaaac ctttcgacga gggggggctg ttcgacaaac tgcgcatggc
     6901 cggagttgag gagctatgat gccgggggct gagatcggtg cgcgcaggat tcgcgtcctg
     6961 cacatcgatg attccgcggt catgaggagc ctgctgcgca tggtgctcga acctcaggct
     7021 tcgatagaga tcgcagctac ggcgtcttcc gggcaggaag ggctggcggc gtttgaacgg
     7081 atccatccgg atcttgtgct gctggacatc gagatgccgg gaatgaatgg gcttgaggtg
     7141 ctctcggcca tccggcgtca tgatcgccgc gtaccggtga tcatgtgcag cacgctgact
     7201 tcacgtggag cccgcatcac catcgaagct ctggcgcgcg gcgcgacgga ttacgtgacc
     7261 aagcctggcg cgcagcacgg agtccgtgag gctgtggaga ccctgagccg cgaactgctt
     7321 ccgaaaatcc tggcgctgtt tccacccgca tctcaccgga ccgcccctcc actcactgac
     7381 gtctcgtgtc cagtgcctcc gcctgcggcg gtgcaccgtc caaagattgt cgttatcggt
     7441 gtttcaacgg gaggcccggc cgcgcttgaa cttctcctgc ccaaaattcc ggctgatttc
     7501 gcgctgccga ttctgatcgt gcagcacatg ccccgtcttt ttaccggact tcttgccgag
     7561 cgcctgaaca gcgtgtgcag cctgcgggtt cgcgaagcgg ctccggatac tcaggccgaa
     7621 gcgggcgtga ttgacatcgc acgcggtgac tggcacctgg aagtccaccg cgacctccgt
     7681 ctgcgcctga atcagaatcc gccggagaat ttttgccggc cgtccgtcga tgtgctcttc
     7741 cggtccgccg cgcaggtctg cgcgggcggc gttctcgggg tcattctgac ggggatgggt
     7801 tcggacgggc tcatcgggtc gcgcgcgatc cgtgcggggg gcgggacggt gttggcacag
     7861 gacggagcga ccagcgtggt ctgggggatg cccggcgcgg tcgcccaggc cggcctggcg
     7921 aataagatcc ttgccctcga tgccattccg gcggagattc tgcgcctgac tgcggccacc
     7981 cccccgcagg ctcggcgcgt ggaggccttt gcctgatgag cgcctccccc atcgattacg
     8041 agtacctgcg cacggtggtt ctgcgcaact caggcaatca ggtcgatgca agtcgcaact
     8101 atctgttcga gtcgcgtctg caggggctgc ttcgcaaccg tggcttcgat tcgctgaccc
     8161 agctggtttc tgctctccgt tcaggcaacg gtcgttccct cgaacgttcg gtcgcagaag
     8221 cgatgacgat caacgagacg agcttctttc gcgacactcc cacgttcgac tacctgcggg
     8281 agacgctgct tcctgctctg attgagagcc ggcgcgacac tcgcacacta cgcttctggt
     8341 ctgcggcgag ttcttccggc caggaggcgt actcgattgc aatgatgctg cgcgagtatt
     8401 ttccgggtct tgccggatgg cgcatcgata tcctgggcac ggatatctcc gccgaaatgg
     8461 tggaacgcgc cagcacaggc cgctaccagc gcatagaagt caaccgcggc ctccctgcac
     8521 gcctcctcgt taaatatttc gtcaggatca atgatgaatg ggaaatccag cccgatctga
     8581 agcggctctg ccgattccag cagcgcaacc tctgcccgct ccccagcctg ccggagacgt
     8641 tcgacatcat ctttctccgc aatgtgatga tctatttcaa tcaggacaca cggcgcgagg
     8701 ttctgcttgg catgcaccgc gcactggcgg cggacggcat tctttttctc ggcctcagcg
     8761 agcagccccg catggactcc cactggcaga cgatgctgac gccgaagacg gtttggtaca
     8821 aaccgattcg ttagcagaaa caacaggcac gacagaagcg caggggaatc cggcgagcag
     8881 gcaggttcct cgctcggtgc gttcgctccc ctggcctttg cgctcttttt gcgctcggct
     8941 atcctcacct caggcatgga cccgctcagc ctcttccacc caattacacg gagctggttc
     9001 cggtcgacgc tgggcgaacc cacttccccg cagcgcctcg gctggccggc gatcgcacgc
     9061 agggagagca cgctgatcct cgccccgacg ggcagcggca aaacgctcgc ggcctttctg
     9121 tcgtgcctcg atcgcctgat gctgcgtgaa cctgcgtcag aaacacgagc caccgaacca
     9181 ggctgtcgcg ttgtctacat cagtccgctg aaagccctgg cggtggatgt ggaacgcaac
     9241 ctgcagacac cgctgcagca gatcgcgccg ctggcacgcc gcgcgggtgt gccgtttcgc
     9301 gaaccgcaga tcgcgatccg caccggcgat acaccgcaga tcgagcgtgc ccggtttcgc
     9361 cgtcatcccg ccgatatcct tatcaccacg ccggaatccc tctacctgct gctgacctcc
     9421 gattccgcca cggctctccg ctcggtcgac acggtgatcg tcgacgagat tcatgcgctg
     9481 gttccgagca aacgtggagc gcatctcgtg cttaccctcg agcggctcca ggcgatctgc
     9541 acccaacctc ttcagcgcat cggcctgtcc gcaacccagc gtccgcttga agaggtcgca
     9601 cggtttctgg gtggatcaca gacttctgag gatccatccg aggtaacggc tcgcccggtc
     9661 acgatcatca atgcgtccgc gcccaaacaa ttacagctca gggttgaagt tccagttgaa
     9721 gatatgaagg cgccggaatc ctcacccgct ttcgggcagg actcgcccgt gaagcgggct
     9781 tctgtctgga cgacgattcc accccgcctg ctggccatca ttcgacgacg acgggccacg
     9841 ctcatcttcg ttaacaatcg gcggacggcg gagcggcttg ccggtctggt gaacgatctg
     9901 gcgcaggaac ctctggctcg cgcccatcat ggctctctcg cggctgcgca gcgagccgaa
     9961 attgaagaac aactcaagaa aggcgggatc cgggctgttg ttgcgacatc ttcgctcgaa
    10021 ctcggcatcg atatgggcgc gattgacctg gtcattcaga ttgagtcgcc gccatccgtc
    10081 gcggcgggga tgcagcgcat cggccgcgcc ggccacaaag tcggggtggt gagcgaaggc
    10141 atcatcttcc cgaaatatcg tgccgatctc atcgcctgct ctgccgtcac gcgcgctatg
    10201 catgaagggc atgtcgaggc tacatcgatg ctccgcaacc ctctcgacgt cctcgcgcag
    10261 caaatcgtcg ccacggtcgc gcaacctccc gcactggccg agccccgact gaaaggtcac
    10321 gccccgcggg aagtctccat ttctgccgat tccgtctatg acctggtccg ccgtgctgct
    10381 ccgttcacga cactgacacg cgccgccttc gaaagcgtcc tcgatttgct ctccggcaag
    10441 tacccctccg aggagttctc cgagctccgc ccgcgtatca actgggaccg cgaggcgaat
    10501 cttctcgctc cgcgtgcggg ctcaaaaagt ctggccatcc tgaacgccgg tacgattccg
    10561 gaccgcggcc tctacggcgt cttcctctca ggcgagcata agaaacctgt tcgcgttggc
    10621 gaactcgacg aggagatggt cttcgagtcg cgtgttgggg aggtctttgc gctgggcgcc
    10681 tccacgtgga ggatcgacga aatctcgcac gaccgcgttc tcgtttcgcc tgcgcccggt
    10741 cagccgggga agcttccctt ctggcatgga gaccaggcgg gacgccctct cgaattcggc
    10801 cgccggatcg gcgcgctggt ccgcgagatt cgcgcgttgc ctcccaacgc ggcgatcagt
    10861 cgactggtgc gcgaacacga tcttgactcc caggcagctg agaatctgct tcaatttctc
    10921 agcgaccagc agaatgtgac tgccactgtg ccagatgacc gctgcatcgt gatcgagcgc
    10981 atccgcgatg agctgggcga ctggcgtgtt tgtgt
//
LOCUS       DASZYN010000499         9941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_341782, whole genome shotgun sequence.
ACCESSION   DASZYN010000499 DASZYN010000000
VERSION     DASZYN010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 9941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9941
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341782"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..2768
                     /locus_tag="VHE33_12850"
     CDS             21..2768
                     /locus_tag="VHE33_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HVT98387.1"
                     /translation="MSKRVAVMLFAGLVALSPASQSQTPQAMDPEFATHVKEWTTKPE
                     FLSPLVDHLPASSAVPSPKEVLGHDIGAPKQLTYYADVLRYYDALAAHSPRVKVLRIG
                     KTEEGRESAIVFVGSEDSISHLDQERQNLARIADPRGLSEAEAQDLIAHTKPIYTLSG
                     GLHSAELGPPEMLMELAYRLATEDSPLIQSIRDHVIVAILPVADPDGRDRSIDWYYAH
                     NVDVTDYEKMSHVPYWGKYIEHDNNRDINYSAEANQNFLRWYLDWHPPIIHDLHESVP
                     FLYIYSGQAPQNPLYDPIVYGELPMLSNWDMTQLTRYGMPGVWTHAYVDAWSPGYVAE
                     MATNHNGLMRFYEIFGNGGATTMERTVSQPNPALLGGGGGFGDMTKRQWYRPNPPYKT
                     VLWSMRDNTNFAETGVLSSLQFVAAFPQVILQDFYVKTRDALAAGTKEAPYGFVLPAN
                     QEDMTRVAFVIHILRMQGIEVGRAKSAVKLSDGDYPAGSFIVKTNQPYGPFAKNLLGK
                     QTDPDPELHTYDDSAWTMGLMTHTEIKPTTDKAVQDIAVDPVDKFMPEGALKDLPAAA
                     VYAVPDHGSPNLVTLRYGLKDVDVRIADKSFTSGGTNFEAGTFLVPASSGDRLKPLAK
                     QLGLDVVALAAAPKVASHESALPRLAVFSTWGGTQDVGWVRYTFDQYKVPYDLIFKER
                     VLQGDLAKDYDLILIPTQARSAKDLVFDIPKSDKPIAYEKTDRFKFLGDYGSSPDISG
                     GMGGTGVAELQKFVEAGGTLVTLGASSAFPPDFGLTPTIDTSNPSAKLYAPGPIVDAD
                     ILQPENPIFYGYSQKTVAVRYANGPLLRMSEEMDKNDVLMRYPGGEKSVLSGLFNGAD
                     DIKGRAAIAVVPEGKGEVVMFTTNPIWRWQNLGEYRMMFNTLMNYRNLAPVPPAAATG
                     K"
     gene            2818..4419
                     /locus_tag="VHE33_12855"
     CDS             2818..4419
                     /locus_tag="VHE33_12855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HVT98388.1"
                     /translation="MEHVDYIVVLFIGLLCAVAAVAGLARRFAISYPIVLVVFGLLCS
                     LLPHVPRVPLPPSLVFLVILPPLLYVAAWQTSWQEFRYNIISISSLAVFLVFFTAFGV
                     AFVARLWLPGFNWRLGFLLGAVVSPTDAVAATSIARKVGMPQRIVDLLEGESLLNDAT
                     GLLALELGIEMVVNGSTPTIAQSLTRFFWLAVVGVVVGLVIGKVVSWLERWVDDGPVE
                     IALSLVVPYAAYLLAEALKGSGVIAVVVCGLYVSRRSSDFFSPGVRLQAIAVWDALEF
                     LLNGFVFVLIGLQLPWVLDGIRGQSKLSLLGYGLAFSAVLVLLRLLWMFPGARLAWWV
                     RTRIAKQLYEKPRGNQVFVVGWTGMRGVVALAAANSLPLTLSDGSPFPARSFIIFLTF
                     ALILVTLVLQGLSLPWLVRVLGLSVPDTQWCEEGEARHLLLQAAIDFLEERRNSAAGE
                     GDLHLYQDLLHSYEHKLSEIENCGPTGTTPTREPRGLTTSQLLLDTIRREREELNLLR
                     ANGRIADNVHRILERELDLSESRLS"
     gene            complement(4448..5266)
                     /locus_tag="VHE33_12860"
     CDS             complement(4448..5266)
                     /locus_tag="VHE33_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98389.1"
                     /translation="MKPAAIPVIVALIALAVSFAVQGQNSPNGSQTPQAPDWSQPGSA
                     SRQQVAPPPDFHRPATTFNTPIGIFDGQADVGSALVPGNASFDAATGAYTVHSAGYNV
                     WYTRDEFRYLWKKMSGDVSLAADIAFPDPKGYGDRKAVLVIRQSLDDDAKEVVVALHG
                     AGMIQLAQRPEKDVRIKDMEFRIGGRGRPGGASPDSLVTVMAQRIGVEKKGDSFSLWV
                     SLEGEPMHEFGPPIELHLDAPFYAGIGFCSHLPTTVDTAVLSKVVLANSAGRLK"
     gene            5412..5879
                     /locus_tag="VHE33_12865"
     CDS             5412..5879
                     /locus_tag="VHE33_12865"
                     /inference="COORDINATES: protein motif:HMM:NF021435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HVT98390.1"
                     /translation="MLRAATLLFLAALPFAGPRDPSGVYTAPVNGQPLCTLNLHITGF
                     RDNVGAAGGLVFASSAGWPENMAKTIVHGGVPIDRRQAELTFQVPPGHYSAVVIHDEN
                     SNMKLDRNVFGIPKEGFGFSSNPHVIFSAPSFQSAEVPVACPATNLEIKLIYK"
     gene            complement(5954..6277)
                     /locus_tag="VHE33_12870"
     CDS             complement(5954..6277)
                     /locus_tag="VHE33_12870"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HVT98391.1"
                     /translation="MTKADLVEEVTKLGDLTRRDSETIVETIFDSVIGALRSGDKIEI
                     RGFGSFRIRQRKPRIGRNPKTGTKVEVPAKKVPYFKPSKELRDLVNPGGKSEPAAAAD
                     PGTPA"
     gene            complement(6416..7438)
                     /gene="sppA"
                     /locus_tag="VHE33_12875"
     CDS             complement(6416..7438)
                     /gene="sppA"
                     /locus_tag="VHE33_12875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HVT98392.1"
                     /translation="MDEPLTPPTGEPSAPAESYPPPPPGYQAAWSAPPSYPTPPPKRR
                     RVWLWVTIGGVLAFGFIVLCSVLLWSVARTLSGGQDGIALGDSIGVIDIDGEILSADV
                     LVDQLRKYDQDDDIKAIVLHINSPGGGAAASQEIYHEVLKIREHKRKPVVASIESVGA
                     SGAYYIASAANTIYANDASVVGSIGVIMEWTNYGDLLSWAKMKPEVIHAGALKDAGDP
                     THAMTPEERVYFQSLVDNMYGQFIADVAKGRNLQADQIKGLATGQVWTGQQALPLHLI
                     DKEGGFRDALLATAKQVGISGEPSVVKPAVHHRSVWDLIDNANDLVHQPQQMLERRPG
                     FYFLWR"
     gene            7623..8384
                     /locus_tag="VHE33_12880"
     CDS             7623..8384
                     /locus_tag="VHE33_12880"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HVT98393.1"
                     /translation="MNPQYTIVIPAYNESARIGAALAEVLRTLDEKGWNAEVIVVNDG
                     STDNTAAIVESFIAKDARIRLLHNGGNRGKGYSVRNGLLHGSGNIVMFTDADLSSPMP
                     EAERLFDAIRDGADVAIGSRWLEVSRQTIHQPLYRQFFGRCFNVVTRMIMRLPFADTQ
                     CGFKAFRRSAAQTIFQLQRIERWGFDPEILFIALKRGYRIREVPVTWGHDERSRISYL
                     RDGIKMLEELIYVRWNSLVGIYDRQVTDLAPAERG"
     gene            8516..9166
                     /locus_tag="VHE33_12885"
     CDS             8516..9166
                     /locus_tag="VHE33_12885"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVT98394.1"
                     /translation="MYHQIFETKQDRREWKGHCGRHQHAGPQWFEGGFAFGQTPGFEG
                     RGRHGFGGFGRGRWDRGFGGGRLFDAGDLKLVILKLLSEEPSYGYQLMKKMEERLAGG
                     YTPSAGVIYPTLTLLEEEGLASVATSADNKKVYTVTPAGLAFLEENKERVEELFARLE
                     EARGGFERGRSPEIMKAFMNLRGAVMARVFRGNVTAEQIKKITEAINAAAKAVDEL"
     gene            9426..>9941
                     /locus_tag="VHE33_12890"
     CDS             9426..>9941
                     /locus_tag="VHE33_12890"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HVT98395.1"
                     /translation="MSAKKIIAVVGATGAQGGGLVRAIASDKGGEFVARALTRKTDSE
                     KAVQLKNLGVEVVSADLDDQKSLEQAFSGAYGAFCVTNYWEHFSPEKELAQARNLAQA
                     AKAAGLRHVIWSTLEDTRRWVPLNDDRMPTLMGKYKVPHLDAKGEANQMFIDAGVPTT
                     LLNTSFYWDNFI"
BASE COUNT         1849 a         2875 c         3156 g         2061 t
ORIGIN      
        1 tttccagagg aagcgagttc atgtctaaac gtgttgctgt gatgctgttc gcaggtctcg
       61 tagccctttc cccagcgtcc caatcccaga ctcctcaggc gatggatccc gaattcgcga
      121 cacatgtcaa ggagtggacc accaaaccgg agttcctgag cccgctcgtc gaccatctcc
      181 cggccagcag cgccgtgccg tcgcccaaag aggtcctcgg gcatgacatc ggcgcgccga
      241 aacagctcac ctactatgct gacgtgctgc gctactatga cgcgctggct gcacactcgc
      301 cgcgggtcaa ggtcctgcgc attggcaaga cggaagaagg ccgcgagtct gccatcgtgt
      361 tcgtcggctc ggaagattcg atttcccacc tggatcagga gcgccagaat ctggcgcgta
      421 ttgccgatcc gcgcggactc agcgaggcgg aggcgcagga cctgattgcg cacaccaaac
      481 cgatctatac gctctccggc ggactgcaca gcgccgagct gggacctccc gagatgctga
      541 tggagctggc gtaccggctt gcgaccgagg attcgcctct catccagagc attcgcgatc
      601 acgtcatcgt cgccattctg ccggttgccg atcctgacgg ccgcgaccgt tcgatcgact
      661 ggtactacgc gcacaatgtc gacgttacgg actacgagaa gatgtcccac gtgccctact
      721 ggggcaaata catcgagcac gacaacaacc gcgacatcaa ctactcagcc gaggcaaacc
      781 agaattttct gcgctggtat ctcgactggc atccgccgat cattcacgat ctgcatgagt
      841 cggtgccgtt cctctacatc tactcgggtc aggcgccgca gaatcctctg tatgacccga
      901 tcgtgtatgg cgagctgccg atgctttcca actgggacat gacgcagctc acccgctacg
      961 gcatgcccgg cgtgtggacg cacgcctacg tcgacgcgtg gtcgcccgga tatgtagccg
     1021 agatggcgac gaatcacaac gggctcatgc gcttctacga gatcttcggc aacggcggag
     1081 ccaccacgat ggagcgtacg gtctctcagc ccaaccccgc tttgctgggt ggcggcgggg
     1141 gtttcggcga tatgacgaaa cgtcagtggt atcgccccaa tcctccctac aagacggtcc
     1201 tttggtcgat gcgcgacaac acgaactttg ccgagaccgg cgttctgtcg tcgctccaat
     1261 tcgttgccgc gtttccgcag gtcatcctgc aggactttta cgtgaagacg cgcgacgcgc
     1321 tggcggccgg cacgaaggag gcgccgtacg gatttgtcct tcctgcgaat caggaggaca
     1381 tgacgcgcgt ggcgttcgtg attcacatcc tgcgcatgca ggggattgag gtgggccgcg
     1441 cgaagagtgc ggtgaagctg agcgacggcg attatccggc gggctcgttc attgtgaaga
     1501 ccaaccagcc ctacggtccg tttgcgaaaa atctgcttgg caagcagacg gatcctgatc
     1561 cggagctgca cacctatgac gatagcgcct ggaccatggg gctgatgacg cacacggaga
     1621 tcaaaccgac gactgataag gcggtgcagg acatcgcagt cgatccggtc gacaagttca
     1681 tgccggaagg ggctctgaag gacctgcccg cggcggccgt ttacgccgtt cccgatcacg
     1741 gttcgccgaa cctggtcacg ctgcgctacg ggcttaaaga cgtcgatgta cggattgccg
     1801 acaagtcctt tacatcgggt ggcaccaact tcgaggcagg gacgtttctg gtaccagcca
     1861 gttccggcga ccggctgaag ccgctcgcga aacaactcgg actggatgtc gtcgcgcttg
     1921 ccgctgcgcc aaaggtggct tcgcacgaat cggcgcttcc gcgccttgct gtcttcagca
     1981 cctggggcgg cacccaggat gtgggctggg ttcgctacac gttcgatcag tacaaagtgc
     2041 cctacgacct catcttcaag gagcgcgttc ttcagggcga cctggcgaag gactacgacc
     2101 tcatcctgat tccaacgcag gcgcgctcgg ctaaggacct ggtgttcgat attcctaagt
     2161 ccgacaagcc cattgcgtac gaaaaaacag accggttcaa atttcttggc gactacggct
     2221 cttcgcctga catctcgggc ggcatgggtg gcaccggtgt ggccgagttg cagaagttcg
     2281 ttgaagcggg cggcacgctg gtgacgctcg gcgcctccag cgcatttcct ccggacttcg
     2341 gactgacgcc gacgatcgat acgagcaatc ccagcgcgaa actctatgcg ccggggccga
     2401 tcgtcgatgc cgacatcctg caaccggaga atcccatttt ttacggctac tcgcagaaga
     2461 cggtggcggt tcggtatgcg aatgggccgt tgctgcgcat gtcggaagag atggacaaga
     2521 atgatgtgct gatgcggtat cctggcggcg aaaagtccgt gctgagcggc ctgttcaacg
     2581 gcgccgacga cattaaaggc cgtgctgcga ttgccgtcgt gccggagggc aaaggcgaag
     2641 tggtgatgtt caccaccaac cccatctggc gctggcagaa cctgggagag taccgcatga
     2701 tgttcaacac gctgatgaac taccgcaacc ttgccccagt accgccggcg gcggcgaccg
     2761 ggaaatagca cactcctcgc tggtctccga taggctattt gctatcggca ttttgcgatg
     2821 gagcatgtgg actacatcgt cgttctgttc atcggcctgc tgtgcgccgt cgcagcggtg
     2881 gctggtctcg cgcgccgttt tgcgatctcc taccccatcg ttcttgtcgt ctttgggctg
     2941 ctctgctcgc tgctgccgca tgtgccgcgc gttccactgc ccccctcgct ggtcttcctc
     3001 gtcattctgc cgccgctgct ctatgttgcc gcgtggcaaa cctcgtggca ggagtttcga
     3061 tacaacatca tctcgatctc aagccttgcg gtttttcttg tcttcttcac ggccttcggg
     3121 gtcgccttcg tggcccggct gtggctgccc gggttcaact ggcgtcttgg ctttcttctt
     3181 ggtgctgtgg tctcgcccac ggatgcggtc gcggcgacgt ccatcgcgcg caaagtgggg
     3241 atgccgcagc gcatcgtcga tttgctcgaa ggcgagagcc tgctcaatga cgccacgggc
     3301 ctgctggcac tggaactcgg catcgagatg gtcgtcaacg gatcgacgcc aacgatcgct
     3361 cagagtctga cgcgattctt ctggctggcg gtcgtgggcg ttgtggtggg gctggtcatc
     3421 ggcaaagtgg tctcctggct ggaacgctgg gtcgatgacg ggccggttga aatcgcactg
     3481 tcgctggtgg ttccgtacgc cgcttatttg ctggcggaag cgctgaaagg gtccggagtt
     3541 attgcggtcg tggtctgcgg actgtacgtc agccggcgca gttccgactt cttttcaccg
     3601 ggggtgcgtc tgcaggctat cgccgtgtgg gatgcccttg agtttctgct gaacggtttt
     3661 gtcttcgtcc tgatcgggct acaactgccg tgggtgctcg acggcattcg cggccaaagc
     3721 aagctgagcc ttctggggta tgggctcgcc ttttctgccg tccttgtgct tctgcgtttg
     3781 ctttggatgt ttccaggagc gcgcctggcg tggtgggtgc gcacgcgcat cgccaaacaa
     3841 ctttacgaga aaccccgggg caatcaggtc tttgtcgttg gctggaccgg catgcgcggc
     3901 gtcgtcgcgc tggccgcggc gaactcgctg cctctcacgc tgagcgacgg cagcccgttc
     3961 cccgcgcgga gcttcatcat ctttctcacc ttcgcgctaa tccttgtcac gctggtgctg
     4021 caggggcttt cacttccctg gctggtgcgg gtattgggtc tgtccgttcc cgacacgcaa
     4081 tggtgtgagg agggcgaagc tcgccacctg ctgctgcagg ctgccatcga ctttctggaa
     4141 gagcgcagga attctgctgc cggggaaggc gatctccacc tttaccagga cctgctgcac
     4201 tcgtacgaac ataagctctc cgagattgag aattgcgggc cgacgggcac gacgccgacc
     4261 cgggagcctc gcgggcttac gaccagtcaa ctgctgctcg acacgattcg gcgcgagcgc
     4321 gaggaactga acctgctgcg cgccaacggg cggattgcgg acaatgttca tcgcatcctc
     4381 gagcgcgaac tcgacctgag cgagagccgg ctgagttgaa agcagggtgc agggtgcggc
     4441 gggttggcta ctttagccgt ccggcgctgt tcgccagtac tacttttgag agcacagctg
     4501 tgtccacggt ggtgggcagg tgggagcaga atccgattcc ggcgtagaac ggcgcgtcga
     4561 gatgcagctc aatgggcgga ccgaactcgt gcatcggctc gccttcgagg ctcacccaga
     4621 gcgagaacga atcgcctttc ttctcgacgc cgatccgctg cgccatcacg gtgaccaagc
     4681 tgtcgggtga agcgccgccg ggccggcctc gcccgccgat gcgaaattcc atgtccttga
     4741 tgcggacgtc cttctccggg cgctgcgcga gctggatcat gccagcgccg tggagggcga
     4801 ccacgacttc ttttgcgtcg tcgtcgaggc tttgccggat caccaggaca gctttgcgat
     4861 cgccgtagcc tttggggtcg ggaaaggcga tgtcggcggc gagcgataca tcgccggaca
     4921 tttttttcca gaggtagcgg aattcatcgc gcgtgtacca gacgttgtag ccggcagagt
     4981 ggacggtgta tgcgcctgtc gccgcgtcga agctggcatt tcccgggacc agtgcgctgc
     5041 cgacatcggc ctggccgtcg aaaatgccga tgggtgtgtt gaaggtggtt gccgggcgat
     5101 gaaagtcggg cggtggagcg acctgctggc gtgatgcgga gccgggctgg gaccagtcgg
     5161 gcgcctgggg tgtttgcgag ccattgggtg agttctgtcc ctggactgcg aaggaaactg
     5221 ccagggcaat cagggccacg atcaccggta tcgccgctgg tttcatgcgc gttgcacctc
     5281 ggacggattg tagctctgcc cgagtcgtat tctgccgggt actgcaaagg tttcgcgggg
     5341 cgaaccagtg acaatggaaa aacaattccg agttatcctg gggctggtcc tccagcgtcc
     5401 aacaaatcgt gatgcttcgt gccgctacgc tgctgtttct cgccgcactc cccttcgccg
     5461 gtccgcgcga tcccagcggt gtctacactg ctcctgtgaa tggacagcca ctttgcacgc
     5521 tgaacctgca tatcaccggg tttcgcgaca acgtgggagc ggcgggaggc ctggtgtttg
     5581 cctcatctgc cggatggccc gagaacatgg cgaaaaccat tgtgcatggc ggcgtcccca
     5641 ttgatcgacg gcaagccgag ctgacgtttc aggttccgcc tgggcactac tccgcggtgg
     5701 tgatccacga cgagaactcg aatatgaagc tggaccggaa tgtcttcggg attccgaaag
     5761 agggctttgg tttctcgagc aatccgcacg taattttctc cgcgccgtcg ttccagagcg
     5821 cagaggttcc ggtcgcgtgt ccggcgacga accttgaaat caaactgatc tacaagtaag
     5881 cgggctacga gtgagaggcg agcaaaaggc gagtgaactg cgagcaaaaa agcgcgtgga
     5941 gcttctagtt tccctacgct ggtgtccccg ggtctgcagc agccgccggt tctgattttc
     6001 cgcctgggtt caccaggtcg cgcaactcct tgctcggctt gaagtaggga accttcttcg
     6061 ccggcacctc caccttcgtt ccggtcttgg gattccgccc aatgcgcggc ttccgctgac
     6121 ggatgcgaaa gctgccgaag ccacgaatct cgatcttatc cccggagcgc agcgctccga
     6181 tgacggaatc gaaaatggtt tcgacaatgg tttcactgtc gcggcgggtc agatcgccga
     6241 gcttggttac ttcctcgacc aggtctgctt tcgtcatggt ggggttggct cccgtgtgcg
     6301 ttggccctct gttcaccata gtagatgcat tctcttcgct tgcaaagtca agcatttgca
     6361 ggatttttag acggaatccc cggggcggcc ggaaccccgg ccaaaggaaa ccggcttacc
     6421 gccacagaaa atagaagcct ggacgccgtt ccagcatttg ctgcggctgg tggacaagat
     6481 cattcgcgtt gtcgatcagg tcccagacgg agcggtgatg cacggccggc ttgacgacgc
     6541 tcggttcgcc ggaaatgccc acctgctttg cagtcgccag cagcgcatcg cggaagccgc
     6601 cctccttatc gatcaaatgc agcggcagag cctgctgtcc ggtccagacc tggcccgtcg
     6661 ccaggccttt gatttggtcg gcctgcagat ttcgaccttt cgccacatcg gcgatgaact
     6721 ggccatacat gttgtcgacg aggctctgga agtaaacgcg ttcctcgggc gtcatcgcgt
     6781 gggtgggatc gcctgcatcc ttcaaggctc cggcgtggat cacctcgggc ttcatcttcg
     6841 cccagctgag cagatcgccg tagttggtcc actccatgat cacgccgatg gagccgacga
     6901 cgctggcgtc gttggcgtag atggtgttgg cggctgaggc aatgtagtag gccccgctcg
     6961 cgcccacgct ctcaatcgac gccacgacgg gcttcctctt gtgctcgcgg atcttcagaa
     7021 cctcgtgata aatctcctgc gacgctgccg cgcccccgcc tggcgaattg atgtgcagaa
     7081 cgatggcctt gatgtcgtca tcctggtcgt atttgcggag ctggtcgacc agcacgtcag
     7141 cagagagaat ctcgccgtcg atgtcgatga cgccgatgct gtcgccgagc gcgatgccat
     7201 cctggccgcc gctgagcgtt cgtgccaccg accacagcag cacggagcaa agcacgatga
     7261 agccgaacgc cagcactccg ccgatcgtga cccacagcca tacccgcctc cgcttcggcg
     7321 gaggggtggg ataggaggga ggggcgctcc aggccgcctg atatccgggc ggcgggggag
     7381 gatagctctc cgcaggcgcc gaaggctcgc cggtcggcgg tgtaagtggt tcatccataa
     7441 gggggagtgt agcaccggcg gcatcccgcc aggcgggagc cctctgttca cgggctcaaa
     7501 acgcaggtaa tataacgatt acattccccc tgaaagatag tttgtatttt gcgaccgccg
     7561 ctggtcgaag ggtgtccaat aggtgacggt gtagtttgaa cgctaaaacg attgagtctc
     7621 ctgtgaatcc tcagtacacg atcgtgatcc cggcatacaa cgaaagcgct cgcattggag
     7681 ccgcgctggc cgaggtgctg cgcacgctgg acgaaaaggg ctggaacgcc gaggtgatcg
     7741 tcgtcaacga tggttccacg gacaacacgg cggcgatcgt cgaatcgttt attgcgaagg
     7801 acgcgcggat tcggctgctg cacaatggcg gcaaccgggg caagggctac agcgtgcgga
     7861 acgggctgct gcacggcagc ggcaacatcg tgatgtttac cgacgccgac ctttcctcgc
     7921 cgatgcccga ggccgagcgg ctgttcgacg ccatccgcga tggggccgat gttgcgattg
     7981 gatcccgttg gctggaggtc agccgccaga cgattcacca gccgctttat cgccagttct
     8041 tcggccggtg tttcaatgtg gtcacgcgca tgatcatgcg gctgccgttt gcggacacgc
     8101 agtgcgggtt caaggcgttc cggcggtctg ctgcgcaaac gatcttccag ctgcagcgga
     8161 tcgagcgctg gggtttcgac ccggagatcc tcttcatcgc gctcaaacgc ggctaccgca
     8221 tccgcgaggt ccctgtgacg tggggtcacg acgaacggtc ccggatcagc tacctgcggg
     8281 acggcatcaa gatgctggag gagctgattt atgtccggtg gaactcgctg gttggcattt
     8341 acgaccgcca agtgactgat ctggcaccgg ctgaacgcgg ctaaggccag ccttttcctt
     8401 tccattgaaa ttgcagcagt tgcggggtat tttagggttg gcgagataaa gatatatttt
     8461 ttggcttgac ttaagatata tcgtgagata cattgtcgat agaaaggaag tctctatgta
     8521 tcaccaaata tttgaaacaa aacaagatcg gcgggagtgg aagggtcatt gcgggcgtca
     8581 tcagcatgcc ggaccccagt ggttcgaagg cggattcgcg ttcggtcaaa cgccggggtt
     8641 tgaaggccga ggccgtcacg gattcggcgg gttcgggcgc ggccgatggg atcggggatt
     8701 cggaggcggg cggttgttcg atgccggcga cctgaaactc gtgatcctga aactgctgtc
     8761 tgaagagccg agctacggtt atcagctgat gaaaaagatg gaggagcggc tcgcgggcgg
     8821 ctacacgccg agtgcggggg tcatctatcc gacgctgacg cttctggaag aagagggcct
     8881 ggcgtcggtt gcgacatcgg cagacaacaa aaaggtctac acggtgacgc cggcggggtt
     8941 ggcgttcctt gaagagaaca aagaacgcgt ggaggagttg tttgcgcggc tggaagaagc
     9001 gcgcggcggc tttgagcgtg gacgctcgcc ggagatcatg aaggcgttca tgaatctgcg
     9061 cggcgccgtg atggcgcggg tgttccgcgg aaacgtgacg gcggagcaga tcaaaaagat
     9121 caccgaggcg atcaacgcag cggcgaaggc ggtcgacgag ctctaaaggc gggcagtagg
     9181 cagtagccaa taggcggttg cccttgaatc ctggcgcgaa ttttgcgcca ggatttgtca
     9241 tgttcgttca tcgagccgaa tagggtgcat cagggtctcg ccgcttcgtt tggctcccgt
     9301 cgacatcgac caaccctgac gcccccgagt ctcagccgtg gttcgggtag gatgaggaac
     9361 gcgcaggcgg tggggcttag tgagctgacc gaaaccgcca ttgcgcaaca cctgaggaga
     9421 aaattatgtc agccaaaaag attattgctg tggtgggagc gacgggcgcg cagggtggcg
     9481 gcctggtccg cgcgattgcg agtgacaagg gtggcgagtt cgttgcgcgg gctttaacgc
     9541 ggaagacgga ctcggagaag gccgtgcaac tgaagaacct tggggttgag gtagtgagtg
     9601 ccgaccttga cgatcagaag agcctcgagc aggccttcag cggcgcgtac ggcgccttct
     9661 gcgtgacgaa ctactgggag cacttttcgc cggagaagga actggcacag gcgcgcaatc
     9721 tggcgcaggc ggcgaaggca gctggactga ggcacgtgat ctggtcgacg ctcgaagaca
     9781 cgcgacgctg ggttccgctg aacgatgacc ggatgccgac gctgatgggc aagtacaaag
     9841 tgcctcatct ggacgccaag ggcgaggcga atcagatgtt catcgacgca ggtgtgccga
     9901 ccacgttgct gaacacttcg ttctattggg acaacttcat c
//
LOCUS       DASZYN010000500         8430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1243207, whole genome shotgun sequence.
ACCESSION   DASZYN010000500 DASZYN010000000
VERSION     DASZYN010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8430
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243207"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..702
                     /locus_tag="VHE33_12895"
     CDS             43..702
                     /locus_tag="VHE33_12895"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT98396.1"
                     /translation="METGSHTTLKAKVGLLDFEPLRIAGLREIFAEQPNFQVIATDWA
                     SAFRDSALDLVVVVLREQAFSLALLARLRSRHPGLKVLVIGTGSEPEKILEAIGAGAK
                     GWLEETASPEQVMHAACAILDGSIWAPRQVLATFVDRALEGSAARRRRSSPRFTEREE
                     EVLQQLVLARSNREIAQALSIREQTVKSYVARLMRKVGVGNRIALSVQAASGAWGEKK
                     P"
     gene            complement(754..1455)
                     /locus_tag="VHE33_12900"
     CDS             complement(754..1455)
                     /locus_tag="VHE33_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98397.1"
                     /translation="MGSERIRFVSFSGIDGAGKSTQIEALRNRLQAAGLSVRTLTFWD
                     DVACFKGVRESAGHTLFGGEKGVGTPEKPINRRDKNVRSRTMTVIRWGIYLADAISLR
                     LAVRRALRSADFLICDRYAWDELANLTLHHWLTRMYIRLIARIVPRPDVSFVLDADPI
                     LARARKPEYPLDFLYECRESYLRLSELVGQITVIPPGPVGEVEAAVLNQVAVEKKPAG
                     RSGAFVENAGGGAAA"
     gene            complement(1495..1986)
                     /locus_tag="VHE33_12905"
     CDS             complement(1495..1986)
                     /locus_tag="VHE33_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98398.1"
                     /translation="MKTAILYWERWIAIALAILLAVPITAVASAQEIPDAPRPQNNTP
                     APAPAVPVPTAPEVASLPDSPAPVPQNEPGQQQPQEQQPANTQPTPPQQQGNPSQPMG
                     TAVAPAQPAGGVAATRPAGAAIAPAKQKRSHTLLIRWSLIIGAAVAVGTVVGLSSASP
                     SRP"
     gene            complement(2024..2383)
                     /locus_tag="VHE33_12910"
     CDS             complement(2024..2383)
                     /locus_tag="VHE33_12910"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVT98399.1"
                     /translation="MAVRKVPDVLSVQQKVAFLHEIAGIMNTSRPRLVLDCSRLRDCD
                     LSTIHLLLHCLEEAMKRNGDVKLAGVPTAAAIRLVSTGVAGLFETFDSTEEAVRSFHQ
                     LPLPREKAQDRRSERAA"
     gene            complement(2447..4420)
                     /locus_tag="VHE33_12915"
     CDS             complement(2447..4420)
                     /locus_tag="VHE33_12915"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HVT98400.1"
                     /translation="MDNGPLAPRSTPHARILSGSFILLLGSGIATVTNFAYNIAVARF
                     LGPTGYGHATAVYTLLILISAGTLSFQIVSAKLVARQDSDEKKRAVYRGLHRGAWGCG
                     LLAGAIFFIYQAPIAAYLKLPTPLLVTWVAVGAAFYVPLGIQRGYIQGVCKFHQLAIN
                     VVVEGVVRLGGSVLLMYLGYGVTGVIAANAGGVAAAYLEAMPWKRVPRIMNPVLFQEG
                     LREIGQALIFFAGQVLINNCDIILVKHFFLPADAGVYAAIALVGRVIYVLSFAVVNTM
                     FPIVAGTSREERRGFRVIATPCLLVLGIGGSLALGLYVTPAWLWMRFFGSGFQMSGPY
                     DLPQLLSLYAVTSTIYCLSVVVITYEMAHKIARISWIPLTASGVVIGGICLFHGSLHE
                     VIWIQLILMIALFALVASMFIFATLTDHGEVEEELSSGPIRILRPVAQEEVIAEFLKN
                     DFNSPAFHEYQQVLRDLVMNPNLADPVENTKRKALLFIRHLALWKELPADTQWYEVEL
                     RSTELDSVRCFPRAHWRKMAQGDFSVTAVAERMRQGIDGAEPMLRGKLDALANRISSG
                     DSEMGVIVLIGLNENEPVTVLDGNHRLMATILSAPADLDRLRFLCGLSPRMSECCWYN
                     TNLVTLTRYAKNVLTHLIHDPEAELARLMESTG"
     gene            4713..5597
                     /locus_tag="VHE33_12920"
     CDS             4713..5597
                     /locus_tag="VHE33_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98401.1"
                     /translation="MRLKAGIPGVSPRILAVYEPWFGHPAHISVGYSSHDPNVLRKQI
                     RAAKKMGIYAFVVDWYGDRDPFNARTYELLQPIAAQEHFHIAMMYDETDHVDGATDES
                     LADFINFRDNHLAPNAPGRDAYLTDNGHPMIFIFPKGGHTDWKRIHDEVGKWNPAPLM
                     IYENIPGPDAADFDGFYAWVQPGSKGYAPDGSNWGQDYLSEFYSTMVSKYPGKIAVGG
                     AWAQFDDSKASWGQNRHIAARCGQTFNDTFNFWRRFYPANQPLPYLMIETWNDYEEGT
                     AVEPGIPTCKSGARQEAR"
     gene            complement(5601..6584)
                     /locus_tag="VHE33_12925"
     CDS             complement(5601..6584)
                     /locus_tag="VHE33_12925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HVT98402.1"
                     /translation="MISVVVPIYNEEENINLFHQAVADALQDIGEEWEAVYVNDGSKD
                     TSLELLRGLQEFDPHVVVVDLSRNWGHMGAISAGLQTARGDAVVLMDGDFQDPPDVIP
                     ELVEAWRGGAQVVVAVRRSRQEKRKVLAALFPLFYKVLGALSDFPIPLNAGIFGLMDR
                     QTIDSINSMREKNRYLPGLRAWVGYSNAVVYYDRQDRLAGEGKLSFLSRIKYAMDAIT
                     SFSYKPLRLCFVLSAASCCIAALAALISLVADASLTSVGWGVSSAVFLVGGAVLLCMG
                     VLGEYLGRVYDEVRQRPLSIINKVHYPSIAGVLENQPEYEEEQPARVLRIA"
     gene            complement(6746..7243)
                     /locus_tag="VHE33_12930"
     CDS             complement(6746..7243)
                     /locus_tag="VHE33_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98403.1"
                     /translation="MMRRILILGTILAASGAFAADQPSVQVEPTNLQGPRPLAKQTAS
                     AVVHDYLQAWQSMAAAMDQNRPELLDRDFVGEAREKLGATVAEQTKFGMRTHYQERSH
                     DIKIIFYSPEGLSIQLVDNIEYDEQLLNNGKVIAVQPIHTRYVAVLTPTEVQWKVRIL
                     QSEPL"
     gene            complement(7240..8112)
                     /locus_tag="VHE33_12935"
     CDS             complement(7240..8112)
                     /locus_tag="VHE33_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98404.1"
                     /translation="MSRTAFIFVCMLSAMALPAVGQDGSLDVPKSVVAGTAFSIQAPG
                     SGKGTLYIAGPGGALKRDVQLGQPIAIAASDLNSAGHYVAVLSTDSSSQTAQFDVVPA
                     SDPAAISFLARPSRLPVNLQNGITGAVYLFDSYRNLITSPVPVSFQLAVGSAPAQSRT
                     VTTKEGAAWTGMNSATKEGNAKFMAQAGSVSATRVIEQVPGEPCGLKMNAHKIGDKLN
                     LVTDPLRDCSGNAVSDGTVVTFTETWQGGQTTVDVPLKRDIAEAMVPAHVGARLSVAT
                     GTVMGNEIRWEGQE"
     gene            complement(8109..>8430)
                     /locus_tag="VHE33_12940"
     CDS             complement(8109..>8430)
                     /locus_tag="VHE33_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98405.1"
                     /translation="DPTSNLIAASADPVVEIQNQKMSQPPYSVFSGDLSNQSGQIVNI
                     AHVLATFYDQSGQLVWVGDQYIDRALQPQTPVPFTISVPEDLARKVSSERAVVATYSN
                     AAFQ"
BASE COUNT         1725 a         2683 c         2358 g         1664 t
ORIGIN      
        1 tcaaaaacag cagggaattg ttctcccaaa attgtaggag ctatggaaac aggcagtcac
       61 accactctta aggcaaaagt tggactactg gatttcgagc cgcttcgaat cgcggggttg
      121 cgggagatct ttgccgagca gccgaatttt caggtcatcg caaccgactg ggcgagcgcc
      181 ttcagggatt cggctctcga tctggtcgtt gtcgtgttgc gggaacaggc cttttccctg
      241 gcattgctgg cccggctgcg ttcccggcat cctggattga aagttctggt tatcggtaca
      301 gggtctgagc ctgagaagat ccttgaggcc atcggcgcgg gtgccaaagg atggctggaa
      361 gagaccgcca gcccggaaca agtcatgcat gccgcatgtg ccattttgga tggctccatc
      421 tgggcgcccc ggcaggtcct ggcgaccttt gttgaccgcg ctctggaagg atctgcggca
      481 cggcgtcgcc ggtcgagtcc gcgcttcacc gagcgcgaag aagaggtcct gcagcagttg
      541 gtgctggcgc gctctaatcg tgaaatcgcc caagcgctga gcatccgcga gcagaccgtg
      601 aagtcctacg tagcacggct gatgcgaaag gttggagtgg ggaatcgtat tgcgctgtcg
      661 gtgcaggctg ccagcggagc ctggggcgag aagaagccct agagcgctcc atggagctta
      721 gttaacagaa tatctgttat cggacgtaat ctcttaggcg gcagccccgc cacctgcgtt
      781 ctccacaaag gccccgctcc ggcccgccgg cttcttttct acagcaacct ggttcaaaac
      841 cgccgcctcc acctctccaa ccggccccgg tggaataacg gtgatttgtc ccaccaattc
      901 gctcagtctc aaatacgact cccggcattc atagagaaaa tccagcggat actccggctt
      961 gcgggcgcgc gccagaatcg gatcggcgtc cagcacgaag ctgacatccg ggcgcgggac
     1021 aatcctggcg atgaggcgta tatacatccg cgtgagccaa tggtgcagcg tcagattcgc
     1081 cagctcatcc cacgcgtagc gatcgcagat aagaaagtcg gcggaacgaa gtgcgcgccg
     1141 aaccgccaga cggagtgaga tcgcatccgc aagataaatc ccccagcgga tgacggtcat
     1201 tgtgcgcgag cgaacgttct tgtcgcgccg gttgatcggc ttctcaggag tgcccacgcc
     1261 cttttcgccc ccaaacagcg tatgcccggc gctctcacgc acacctttga aacaagccac
     1321 atcgtcccag aacgtcagcg tgcgaacgct caacccagcc gcctgaagcc ggttccgcag
     1381 cgcctcgatc tgcgtgcttt tcccggcccc gtcaattccg gaaaaactga cgaacctgat
     1441 tcgttctgag cccatcgtgc ttcctcccga gaaaccagtt ccctgttcgc cttcttacgg
     1501 acggcttgga cttgcgctcg acaatccaac caccgtaccg accgcgaccg cggcgccgat
     1561 gatcagactc cagcgaatca gcagtgtatg gctgcgtttc tgtttcgccg gtgcaatcgc
     1621 cgcgccggcc ggccgcgtcg ccgcaacgcc tcctgccggc tgtgccggcg ctactgccgt
     1681 tcccatcggc tgcgatggat tgccttgctg ctggggtggg gtaggttgcg tgttcgcagg
     1741 ttgctgttcc tgaggctgct gctgccctgg ctcgttctgc ggaactggag ccggactgtc
     1801 cggcagagac gctacctcgg gcgcggtcgg taccggcacg gctggtgccg gtgcaggcgt
     1861 gttgttctgc ggccgcggcg cgtccggaat ctcctgcgcc gacgccacag ccgtaatcgg
     1921 tacggccagc aggatcgcca gcgcaatcgc aatccatcgt tcccaataca gtattgctgt
     1981 cttcatatcg ttctcccgat ccggactttc tgtatcacgc gcactaggca gcgcgctcgg
     2041 agcggcggtc ctgggccttt tcccgcggca gcggaagctg atggaagctc ctcaccgctt
     2101 cctccgttga atcgaacgtt tcgaatagac ccgcgacacc ggtactgacc aggcggatgg
     2161 ctgctgctgt cggcaccccc gccagcttca cgtcgccatt ccgcttcatc gcctcttcca
     2221 gacaatgcag cagcagatgg atggtggaga ggtcgcaatc ccgcagccgc gagcagtcga
     2281 ggaccagccg cgggcggctc gtattcatga tccccgcgat ctcgtgaaga aacgccacct
     2341 tctgctgcac gctaagaaca tccggcacct ttcgcacggc caccggtcgc attcctgtcg
     2401 gtatctcggt caacttcata ccctcctttt ggtgggccca aaaaccttag cccgtgcttt
     2461 ccatgagacg cgccagctcc gcctccggat cgtgaatcag atgcgtcaga acgttctttg
     2521 cgtaacgggt cagcgtcacg agattcgtgt tgtaccagca acattcgctc attcgtggag
     2581 acagaccgca aagaaaacgc agccggtcga ggtccgcagg agcagaaagg atcgtggcca
     2641 tcagacggtg atttccatcc agcaccgtta ccggctcgtt ctcgttgaga ccgatcaaaa
     2701 cgatgacgcc catctccgaa tccccgctcg agatccgatt ggcgagggcg tcgagcttgc
     2761 cgcgcagcat cggctcggcc ccgtcgattc cctgccgcat ccgctcggcc accgcggtca
     2821 ccgaaaaatc tccctgcgcc atcttccgcc agtgcgctcg gggaaaacat cgcaccgagt
     2881 ccagttcggt gctccgcagt tccacctcat accactgtgt gtcggcgggc agctccttcc
     2941 acaacgccag gtgacggatg aacagaagcg cctttcgctt ggtgttctcg accggatctg
     3001 ccagattcgg attcatcacc agatcgcgca gcacctgttg gtattcgtgg aacgcgggac
     3061 tattgaagtc attcttcagg aactccgcga tcacttcttc ctgcgcaacc ggccgcagaa
     3121 tccgaatcgg acctgagctg agttcctcct cgacctcccc atggtcggtg agcgtcgcga
     3181 agataaacat cgacgcaacc agcgcgaaca gcgcgatcat caggatgagc tgaatccaga
     3241 tgacctcatg cagggatcca tgaaacagac agatgccgcc aatcacgacg ccgctggcgg
     3301 taagcggaat ccagctgatc ctcgcgatct tgtgcgccat ctcatacgtg atcaccacca
     3361 cgctgaggca ataaatcgtg gaagtgaccg cgtacagcga taacagctga ggcagatcgt
     3421 acggcccgct catctgaaag ccgctgccaa aaaagcgcat ccacaaccac gccggagtca
     3481 cgtacaggcc cagagccagg ctgccgccga tgcccagcac aagcaaacat ggggttgcga
     3541 tgaccctgaa tccgcggcgc tcctcgcggc tggtgccggc cacgatggga aacatggtgt
     3601 tgaccacggc aaaggacaaa acgtagatca cccgtcccac cagggcaatg gccgcgtaaa
     3661 cgccggcatc cgcgggcagg aagaagtgct tgaccaggat gatgtcgcag ttgttgatca
     3721 gcacctgccc ggcaaagaaa atgagtgcct ggccgatctc gcggagtccc tcctgaaaca
     3781 gaacaggatt catgatccgc ggaacccgtt tccacggcat cgcctccaga taggccgcag
     3841 ccacaccgcc tgcattcgct gcgatcactc ccgtcactcc gtagccgagg tacatcagca
     3901 gaacggagcc acccagccgc acaaccccct cgacgaccac attgatcgcc agctgatgga
     3961 atttgcatac accctggata tagccgcgct gaatgccgag tggaacgtag aacgcagccc
     4021 cgaccgctac ccaggtgacc agcagcggcg tcggcagttt gaggtaggcc gcgatggggg
     4081 cctgatagat aaagaaaatc gcgccggcga ggaggccgca accccaggcg cctcgatgaa
     4141 gcccgcggta gaccgcgcgt ttcttctcgt ccgaatcctg ccgcgcgacc agctttgcgg
     4201 agacaatctg aaacgaaagg gttccggctg aaatcagaat cagcagggtg taaacagcag
     4261 tggcgtgtcc ataccccgtg ggacccagaa atctggctac cgcgatgttg taggcaaaat
     4321 tcgttacggt cgcaattccc gaaccaagaa gcagaatgaa acttcctgac aggattcgcg
     4381 cgtgcggggt actgcgtggt gccagggggc cgttgtccac aatctggggc tccggtatta
     4441 cgatgggacg cctcgcatca ataaatgctc gcgaggcggg cacaaaacgg atgaaacaca
     4501 ccccgagcgt gccgccgcgt gagactgagc acttggatgc ttcacttccc ctgctggctg
     4561 ctcttctaaa tcaggtcttt ccggctcagg gacaaccact tgcgcccggt tggcgtctta
     4621 cactttttca ctaaatgata cgaacgttca ctgcagccct tgctgtcctg ctgctcatgc
     4681 atggatgctc cgactcgacg ccgcgaaaag ccgtgcgtct gaaggctggc attcccggtg
     4741 tttccccccg cattctggcc gtctacgagc cctggttcgg ccatcccgcc cacatttccg
     4801 ttggctattc gtcgcacgat cccaatgtgc tgcgtaaaca gatccgtgcc gcaaaaaaga
     4861 tggggattta cgcctttgtc gtcgattggt acggcgaccg cgaccccttc aatgcccgca
     4921 cgtacgaact gttgcagccc atcgccgccc aggaacactt ccacatcgcg atgatgtacg
     4981 acgaaaccga ccatgtggac ggcgccaccg acgaatccct cgccgatttc atcaatttcc
     5041 gtgataacca cctggccccg aatgcgccgg gacgcgatgc ctacctcacc gacaatgggc
     5101 atccgatgat ctttatcttc cccaagggcg gacacaccga ctggaagcgg atccacgacg
     5161 aggtcggcaa atggaatccg gctccattga tgatctacga aaacatcccc gggccggatg
     5221 ccgctgactt cgatggcttt tatgcgtggg tgcaacccgg cagcaagggc tatgcccccg
     5281 acggctcgaa ctggggccag gattatctgt cggagttcta ctccaccatg gtttcgaaat
     5341 atcccggcaa aattgccgtc ggcggcgcct gggctcagtt cgatgacagc aaggcctcgt
     5401 ggggtcaaaa ccgccacatt gcagcccgct gcggccagac tttcaacgac acctttaact
     5461 tctggcgccg cttctacccg gccaatcagc cgctgcccta cctgatgatt gaaacctgga
     5521 acgattacga ggaggggacc gccgttgaac cggggatccc cacctgcaaa tcgggagcgc
     5581 gacaggaagc gcgctgaccg ctacgcgatc cgaagtaccc tggccggctg ttcttcttcg
     5641 tactcgggtt gattttccag aacgcccgcg atcgacggat agtggacctt gttgatgatc
     5701 gacagcggcc gctgacggac ctcgtcatag acccggccga gatactcgcc aaggactccc
     5761 atgcacagca gcactgcccc gccaaccagg aacacagccg acgatacgcc ccagcccaca
     5821 gaggtcagcg aagcgtctgc caccaggctg atgagcgcag ccagagccgc aatgcagcac
     5881 gaagcagccg aaagcacgaa gcacagccgc agcggcttgt agctgaagct ggtaatcgcg
     5941 tccatcgcgt acttgatacg gctcaggaac gacagcttgc cctctccggc gagacggtcc
     6001 tggcgatcgt aatacaccac ggcgttggag taacccaccc aggcacggag gccaggcaaa
     6061 taccggttct tttcccgcat ggaattgatg gagtcgatgg tttggcgatc catcaggccg
     6121 aagatgccgg cattgagcgg aattggaaaa tcggacagtg cccccagcac cttgtagaag
     6181 agcggaaaca gcgcggcaag aaccttgcgc ttctcctggc ggcttctgcg tacggccacc
     6241 accacctgcg cgccgccgcg ccaggcttca accagttcag gaatcacatc cggaggatcc
     6301 tgaaaatctc catccatgag gacaacagca tccccgcgag cggtttgcaa cccggccgag
     6361 atcgctccca tatggcccca gttgcgcgaa agatcgacca ccacgacgtg gggatcgaac
     6421 tcctgcagtc cacgcagcag ctcgagcgac gtgtctttcg acccgtcgtt cacgtacacc
     6481 gcttcccatt cctcaccaat gtcctgcaaa gcgtcagcga ctgcctgatg gaagaggttg
     6541 atattctctt cctcgttata aatcggtacg acgaccgaaa tcatttacgt cctcctgggg
     6601 tgtgccgcat ggcggtcgcg gacgatcagt gaaccgccca aaatttactt gcttggtctt
     6661 gccgttccgt cttacgtgca caacgtgcgt gcggccctgc gtgcttgacg tgcgaaacgt
     6721 tccctggcct gtgcagcttc cctggctaca gcggttccga ctgcagaatc cttactttcc
     6781 actgcacttc tgtcggcgtc agtacggcaa catagcgggt gtgtatcggc tgaactgcta
     6841 taaccttgcc gttgtttaga agctgttcat cgtattcgat gttgtctacc agctgaatgg
     6901 atagcccctc gggtgagtaa aaaataattt ttatgtcgtg cgagcgttcc tggtaatggg
     6961 tgcgcatgcc gaacttcgtc tgttcggcga ccgtcgcccc cagcttttcc ctcgcctcac
     7021 ccacaaaatc ccgatccagc agctccgggc gattctggtc catcgcggcc gccatgctct
     7081 gccaggcctg aagataatcg tggaccaccg ccgatgcggt ctgctttgcc agcggccgcg
     7141 gtccctgcag gttcgtcggt tctacctgca ccgacggctg gtccgcggca aatgccccgg
     7201 aagcggcaag aatcgtcccc aggatcagga ttcggcgcat cattcctgcc cctcccaccg
     7261 aatctcgttc cccatcaccg tacccgtcgc caccgaaagc cgtgccccca cgtgcgccgg
     7321 gaccatggcc tccgcaatgt ctcgcttgag tggcacatca acggtggtct gaccgccctg
     7381 ccacgtttcc gtaaaggtca caactgtccc atcgctgacg gcattgccgc tgcagtctcg
     7441 taacggatcc gtcaccaggt tcagtttatc tcctatcttg tgcgcgttca tcttcagacc
     7501 gcagggttcg cccggaacct gctcgatcac ccgcgttgcc gagacgctgc cggcctgcgc
     7561 catgaatttc gcgtttccct ctttcgtggc ggagttcatg ccggtccacg ctgccccttc
     7621 tttggtcgtg accgttcggc tttgcgccgg tgccgacccg accgccagct ggaagctcac
     7681 gggaaccggc gaagtgatca ggttccggta ggaatcaaat aggtacaccg ctcccgtgat
     7741 gccattctgc agattgaccg gaagacgcga tggccgcgcc aggaaactga tcgcggcagg
     7801 gtcgctggcc ggaacaacgt caaactgggc tgtttgcgac gacgaatcgg tggaaagaac
     7861 agcgacatag tgcccggcgc tgttcagatc gctggccgca atggcaatcg gctgccccag
     7921 ttgcacgtcc cgcttcaagg ctccacccgg cccggcaatg tagagggtgc ccttgccgct
     7981 ccctggggcc tggatcgaga acgccgtccc tgcgaccacc gacttcggca cgtccagaga
     8041 tccgtcctgt cccacggccg gcagagccat ggccgagagc atacagacga aaatgaaagc
     8101 agtgcggctc attggaatgc ggcgttgctg tacgtagcga cgacagccct ttcgctgctg
     8161 acttttctcg ccagatcctc gggaacggag atggtgaagg gtacgggcgt ttgcggctgc
     8221 agcgcacgat cgatatattg atcgcccacc cacaccagct gaccgctttg gtcgtaaaaa
     8281 gtcgcgagta cgtgggcgat attcacgatc tgtccgctct ggttcgaaag atcgccactg
     8341 aacaccgagt atggaggctg gctcattttc tgattctgaa tctccaccac cggatcggcc
     8401 gacgctgcga tcagattcga cgtcggatcc
//
LOCUS       DASZYN010000501         7530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_277603, whole genome shotgun sequence.
ACCESSION   DASZYN010000501 DASZYN010000000
VERSION     DASZYN010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277603"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VHE33_12945"
     CDS             complement(<1..390)
                     /locus_tag="VHE33_12945"
                     /inference="COORDINATES: protein motif:HMM:NF014646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HVT98406.1"
                     /translation="MPDYALMNQYLYEGNADKVEQMTRDALAEGRTVQEVLNEGLIAG
                     MSVVGEDFKHNVLYVPEVLIAARAMKAGMAVLKPLLTSKDSTTRPKGVLLMGTVRGDL
                     HDIGKNLVSMMAEGAGFEVHDIGVDQSL"
     gene            complement(401..1474)
                     /locus_tag="VHE33_12950"
     CDS             complement(401..1474)
                     /locus_tag="VHE33_12950"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HVT98407.1"
                     /translation="MLPEQWETFKRAARREKMEKIPMALIVDSPWIPGYLGIQHLDYF
                     LDPELWFQSNLKIMQEFPDIIFIPSWWMEYGMAAEPSVLGSKIKFWQDNTPSEYHTLY
                     RLEDIDNFPEYEVEADGFAGLTLHRIKMARQRIFDAGYILPMVTSRGPLCTAGFVRGT
                     TEFMIDLVENPKGAHKLIDLCTRVIIDWLKAQQKVMGDKVESIFILDDIVGFVNEEHY
                     LEFAHPYLKRICDAFPKDWVKLYHNDAEVDACLDHLPDTGFNVLNWGKQRHITEVKRR
                     VGDRMCLMGNVNPLEIGVRGTPEEVKDETLEVLEGSGGEGIILSVGGGTSPGMPRENI
                     LAMLEALNEFNAKRAAGAVASVR"
     gene            complement(1753..2970)
                     /locus_tag="VHE33_12955"
     CDS             complement(1753..2970)
                     /locus_tag="VHE33_12955"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT98408.1"
                     /translation="MANSAASGPRFTVLCFGFVLCGMVTVLPGPLLPVMAARWGLRDV
                     QSGAFFAALFLASTVASIFSPRRLRRNLPLGYASMTAGVALLIVAGQVSAGSLGHALA
                     LAAFGLVGLGIGLSVTATNLMVGVTVAEPGIRARRISIVNLWWGIGAVVCPWLIARSE
                     RAGGVPELLAVLAAGTAGMFVALLPQWRETEPARHGEGRESRASQAGILTFFAAFLFL
                     YVGVETVVGGWITTYAHRFSGMTVERAGLIVSLYWVALLAGRWAGAWALKTVPEQAVQ
                     LPALALALAAVAGLVTPHATGIVLVAVAVAGAGFGPVFPVAVSRMLGRVSDHRNTGWV
                     FAVTASGGAVLPWLTGLVSTRTGSLRTGFVVPVAALAAIVLLAAGENRVLGRPGGPAG
                     SGSFSKVPNSEKS"
     gene            complement(3020..4129)
                     /locus_tag="VHE33_12960"
     CDS             complement(3020..4129)
                     /locus_tag="VHE33_12960"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HVT98409.1"
                     /translation="MARRKAAAKKAVPGSEGSGEPAKREFLPPRPISLRTLGDYLDLS
                     PATISLVLNNAPGVRSIPQETRDRVTAAAKKFEYRPSFFARSLRRRQSFSVGVLVPEL
                     SDGYSVLVMSGIEEVLIEEGYFYLTASHRRKADLIEEYPGLLMDRAVEGFVAIDTALQ
                     HRLPLPVVAVAAHKPLPDVTRIILDHARAAELTLKHLYQLGHRQIAFMRGQTFSSDSD
                     DRWKSLMAVAKDLGIEVPPELVVQLEQNLSSPELGYPVVPKLLASKRPFTALVSYNDI
                     AAIGAIRSLRDAGLRVPEDVSVIGFDDIQGAAYHTPSLTTIRQPLSSMGNTAARLLLQ
                     RIRGVQDVPEQVSIVPELIIRESTLPPNPSRMKKR"
     gene            4256..6280
                     /gene="mrdA"
                     /locus_tag="VHE33_12965"
     CDS             4256..6280
                     /gene="mrdA"
                     /locus_tag="VHE33_12965"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HVT98410.1"
                     /translation="MESPSAVLNRDEKLPPFRLATIQYLIAAILAVLVFGLWRLQVVA
                     GQNYHALAEANRIRKVPILAPRGKIFDREGRLIVDNYPSVSCFLVREQGHDINPDLPL
                     IARGLHMTVDQIDAILKKYRLAPKYQPLPLKQDITPDEEEFIEAHKDELPELDTIEEQ
                     RRLYPRDGFAAHLLGYVGEVSEEMLNDPRYAMYEPGDVVGRSGVEATYDGILRGEDGS
                     EDVVVDSHGREVGRLGTEPAKPGQSLRLTIDLDVQRAAENALGDRNGAMIAIDPHTGE
                     VLALVSRPAFDPNAFAVRITRNEWNKYITDPDHPLMDKAIQAQLAPGSTFKIIMALAG
                     LEEGVAQDMTVHCAGGVNLFGTYQRCWVRSGHGAVNVDKAIYQSCDVYFYTLAARLGI
                     DKIAFWAHTVGLGYKTGIDLPDEASGTMPSTEWKLKTFHQKWYPGEVTSVGIGQGAVA
                     ATPVQMVRALAGIASGGVLHRPHVLFPDELKPDVYKSIIDAYPGSADSTFHMDPATWE
                     TITDAMALVPTPIGTDNSAHLEGIDFAGKTGSAQTMSNALAARLGHAHSVKDNAWFVG
                     FSPRRNPDIAVCVLFEAGEHGKLAGRLASRVIAAYVDKQRTLNHNLIAQQPKPVQVGA
                     MWSDPANPNGMGGKPLPLKPDQKSNPELASIRAGHYTVPAQPADSQSHPR"
     gene            6687..6884
                     /gene="rpmI"
                     /locus_tag="VHE33_12970"
     CDS             6687..6884
                     /gene="rpmI"
                     /locus_tag="VHE33_12970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HVT98411.1"
                     /translation="MPKLKTHRGAAKRFKKTGTGKIKRGQSKMRHILTSKEVKTKRHL
                     RKNTLVSDADYAKVARMIPYA"
     gene            7025..7393
                     /gene="rplT"
                     /locus_tag="VHE33_12975"
     CDS             7025..7393
                     /gene="rplT"
                     /locus_tag="VHE33_12975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HVT98412.1"
                     /translation="MPRVKRGTKRSDRRKKILKRASGYFLTKSKLYQAAQEAVERGLK
                     FAYSGRKGKKRQYRSLWIVRINAAAKANGISYSQLINGLKKAGVELDRKILADLAVND
                     AAAFTALCGQAKAAQKPAAA"
BASE COUNT         1548 a         2810 c         1856 g         1316 t
ORIGIN      
        1 caggctttgg tccacgccga tatcgtgcac ctcgaacccc gcgccctccg ccatcatgct
       61 caccagattc ttcccgatgt catgcagatc gccgcgcacc gtgcccatca gcagcacgcc
      121 cttcggcctt gtggtgctgt ctttcgacgt cagcagcggt ttcagcaccg ccatacccgc
      181 cttcatcgcg cgcgccgcaa tcagcacctc cggcacgtac agcacgttgt gcttgaagtc
      241 ctcgcccacc acgctcatcc cggcaatcag cccttcgttc aggacttcct ggacggtccg
      301 gccctcggca agcgcatccc tggtcatttg ctcgacctta tcggcgttgc cttcgtacag
      361 atactggttc atcagcgcgt aatcgggcat cgatttttcc ttagcgaacg ctcgccaccg
      421 cgccggcggc acgtttcgca ttgaattcgt tcagcgcttc gagcatggcc aaaatattct
      481 cccgcggcat tcccggactc gtgccgccgc ccaccgaaag aatgatcccc tcgccgcccg
      541 acccctccag cacctccaga gtctcgtcct tcacttcctc cggcgtgccg cgcacgccaa
      601 tctccagcgg attcacattg cccatcaggc acatccggtc gcccacgcgt cgcttcactt
      661 ccgtgatgtg ccgctgcttg ccccagttca gcacgttgaa tcccgtatcc ggcagatgat
      721 caagacacgc atccacttcg gcgtcgttgt ggtagagctt cacccagtcc ttcgggaacg
      781 catcgcagat tcgcttcagg taaggatgcg cgaattccag gtagtgctcc tcattcacga
      841 acccgacgat gtcgtccaga atgaaaatgc tctcgacctt gtcgcccatc accttctgct
      901 gcgccttcag ccagtcgatg atcacccggg tacaaagatc gatcagtttg tgcgcgccct
      961 tcggattttc gaccaggtcg atcatgaact ccgtcgtgcc ccgcacaaac cccgccgtgc
     1021 acagtggtcc gcgcgacgtc accatcggca aaatgtatcc cgcgtcgaag atccgctgcc
     1081 gcgccatctt gatccgatgc agcgtcagcc ccgcaaatcc atcggcctcc acctcgtact
     1141 ccggaaaatt gtcgatgtcc tcgagccgat acagtgtgtg atattcgctc ggcgtgttgt
     1201 cctgccagaa cttgatcttc gaccccagca ccgacggctc cgccgccatc ccgtattcca
     1261 tccaccacga cggaataaag atgatgtccg gaaactcctg catgattttc aggttcgact
     1321 gaaaccacag ctccggatcg agaaaataat ccaggtgctg gatccccaga taccccggaa
     1381 tccaggggct gtcgacaatc agcgccatcg ggattttctc catcttttcc cgccgtgccg
     1441 cgcgcttaaa tgtttcccat tgttctggca gcatactttt tcctcccgcc cgccgtcagg
     1501 atccagaagt tccgcccagc tcagaaaaat cgcggcgcgt acgacgcttc tctgccagcc
     1561 aacacccttg gcaggctctc ccaatgccga tattgtttac cataacaaca aatcattagg
     1621 caaagctatt ctttcgcctt ccggccaggc cccgccgcgc ccaaacgccg ccttcacagc
     1681 cgtcccttgc gaatcccacc atgttcgcat gagcctgcca tcgtccgcgc cgctccgtgc
     1741 gcctcgtcca gcttaggatt tctcgctgtt cggcaccttt gagaacgacc ccgaacccgc
     1801 cggccctccc ggacgcccca gcacccggtt ctccccagcc gccagcagca cgatcgccgc
     1861 cagcgctgcc accggcacca caaaccccgt ccgcagcgag ccggtcctcg tcgaaaccag
     1921 ccccgtcagc cacggcagta ccgcgccgcc gctcgccgtc accgcaaaca cccatcccgt
     1981 gttgcgatga tcgctcaccc gccccagcat ccgcgaaacg gcaaccggaa ataccggccc
     2041 gaaccccgct ccggctaccg ccacagcaac aagcacaata cccgtcgcgt gcggcgtcac
     2101 cagcccggcc accgcggcca gcgccagtgc cagtgccggc aactgcaccg cctgctccgg
     2161 caccgtcttc agggcccacg ctcccgccca gcgcccagcc agcagcgcca cccagtacag
     2221 cgatacgatc aggcccgctc tctccaccgt catgccgctg aatcgatgcg catacgtggt
     2281 gatccacccg cccaccaccg tctccacccc cacatacagg aaaagaaaag ccgcaaaaaa
     2341 agtaagaatt ccggcctgcg acgcacgcga ctcccgcccc tccccgtgcc gtgccggctc
     2401 tgtctcacgc cactggggca gcagcgcaac gaacataccc gccgttcccg ccgccagaac
     2461 cgccagcaat tccggcactc ctcccgcgcg ttcgctcctt gcaatcagcc atggacatac
     2521 gaccgccccg atcccccacc agagattcac gatcgaaatg cggcgcgccc gaatccccgg
     2581 ttctgcaacc gtcacgccca ccatcaaatt tgtcgccgtc accgaaagac cgatgcccag
     2641 ccccaccagc ccgaacgccg ccagcgccag cgcatgcccc agcgatccag cggacacctg
     2701 tccggcaacg atcagcagcg ccactcccgc ggtcatcgat gcatagccca gcggcagatt
     2761 tcgccgcaac cgccgcggag agaatatcga tgccaccgtc gaggccagga ataatgcggc
     2821 gaagaacgcc cccgactgca catcccgcag cccccagcga gcagccatca ccggcaacag
     2881 cggtcccggc agcacggtca ccatgccgca cagcacaaac ccaaaacaca aaactgtgaa
     2941 tctgggaccc gacgctgcag agttggccac gactgccgat tctaccggcc cctgaacgct
     3001 gaacaccgaa tgctgaacgc tatcttttct tcatccgcga cgggttcggc ggcagcgtcg
     3061 attcgcgaat gatcagttcg ggcacaatcg atacctgttc cggaacatcc tgcacccctc
     3121 gaatcctctg cagcagcagc cgtgctgccg tgtttcccat actgctcagc ggctgccgga
     3181 tcgtggtcag gctcggtgtg tgatacgccg cgccctggat gtcatcgaac ccgatcacgg
     3241 agacgtcttc cggcacacgc agaccggcat cccgcagcga tcggatggcc ccaatcgccg
     3301 caatgtcgtt gtacgacacc agcgcggtaa acggccgctt cgacgccagc agcttcggaa
     3361 ccaccggata tcccagttcg ggcgacgaca ggttctgctc cagttgcaca accagttccg
     3421 gaggcacctc aatccccagg tcctttgcca ccgccatcag gctcttccac cggtcgtcgg
     3481 aatccgagct gaacgtctgt ccgcgcataa aggcaatctg acgatgcccc agctgataca
     3541 ggtgcttcag cgtcagctcc gcggcacgcg catgatccag aatgattcgc gttacatccg
     3601 gcaacggctt gtgcgcagcc accgccacca ccggcagcgg taaccgatgc tgcagagcgg
     3661 tgtcgatggc cacaaaacct tctaccgcgc ggtccatcag cagccccgga tactcttcga
     3721 tcaggtccgc cttccgccgg tggctggcag tcagatagaa gtacccctct tcaatcagca
     3781 cttcctcgat gccgctcatc accagcactg agtagccgtc gctcaattcg ggaaccagca
     3841 cgcccaccga aaagctctgc cgccgccgca gcgaccgcgc aaaaaagctc ggccgatact
     3901 cgaacttctt cgccgccgcg gtcacccggt cccgcgtctc ctgcggaatc gaccgcactc
     3961 ccggcgcatt gttcagaacg agagatattg ttgccggcga cagatccaga tagtcgccca
     4021 gcgtccgcaa actaatcggt cgcggtggca gaaactccct ctttgccggt tcgcccgatc
     4081 cctcggaacc cggtaccgct ttcttcgccg ctgctttcct gcgtgccatt cccaagttct
     4141 aacgttactg acgcgaatct acaactaaaa tgccaccctg tccccgcttg gattcgcgct
     4201 atccttatat cacccctttc gcaaaggatg gcccgctccc ggcaagccgc gggccgtgga
     4261 gtctccctca gccgtgctca accgcgacga aaaactgccc cccttccgtc tcgccaccat
     4321 ccagtacctc atcgcggcga tcctcgcagt tctcgtcttt gggctgtggc ggctgcaggt
     4381 ggtcgccggg caaaactacc acgcgctcgc cgaagccaat cgcatacgca aagttcccat
     4441 cctcgccccc cgcggcaaaa tcttcgaccg cgagggccgc ctgatcgtcg acaactaccc
     4501 ctccgtctcc tgcttcctcg ttcgcgaaca aggccacgac attaaccccg acctgcctct
     4561 catcgcccgc ggcctgcaca tgaccgtcga tcagatcgac gcgattctca aaaaataccg
     4621 actcgctcct aaataccagc ccctcccgct caaacaggac atcacgcccg acgaagaaga
     4681 attcatcgag gctcacaagg acgagctgcc cgagctcgac accatcgaag aacagcgccg
     4741 cctctacccc cgcgacggtt ttgctgccca cctcctgggc tacgtcggcg aagtcagcga
     4801 agaaatgctc aacgacccgc gctacgccat gtacgagccg ggcgatgtgg tcggccgctc
     4861 cggcgtcgaa gccacttacg atggcatcct gcgcggcgaa gacggctcgg aagacgttgt
     4921 cgtcgacagt cacggccgcg aagtcggccg tctcggcacc gagcccgcca aacccggcca
     4981 gagccttcgc ctcaccatcg atctcgacgt ccagcgcgcc gccgaaaatg ccctcgggga
     5041 tcgcaatggc gccatgatcg ccatcgatcc gcataccgga gaagtgctcg ccctcgtcag
     5101 ccgcccggcc ttcgatccca atgcgtttgc ggtccgcatc acccgcaatg agtggaacaa
     5161 atacattacc gaccccgacc atcccctgat ggacaaggcc attcaggcgc agctcgcccc
     5221 cggctcgacc ttcaagatca tcatggcgct cgccggcctc gaagaaggcg tcgcccagga
     5281 catgaccgtg cattgtgcgg gcggtgtgaa cctcttcggc acctaccagc gctgctgggt
     5341 ccgcagcggc cacggcgccg tcaacgtcga caaagccatc taccagtcct gcgacgtcta
     5401 cttctacacc ctcgccgcgc gcctcggcat cgacaagatc gccttctggg cccacaccgt
     5461 cggcctcggc tataagaccg gtatcgatct tcccgatgaa gcctccggta ccatgccctc
     5521 caccgagtgg aagttgaaga ccttccacca gaagtggtat cccggcgaag tcacttcagt
     5581 cggcatcggt cagggggccg tcgccgcaac cccggttcaa atggtgagag ccctcgccgg
     5641 catcgcctcc ggtggcgttc ttcaccggcc ccacgtcctc tttcccgacg agctcaagcc
     5701 cgacgtctac aagtccatca tcgacgccta ccccggctcc gcggactcaa ccttccatat
     5761 ggaccccgcg acctgggaaa ccatcaccga cgccatggcc ctggtgccta cgcccatcgg
     5821 gaccgacaac agcgctcacc tcgaaggcat cgacttcgct ggcaagacgg gcagcgccca
     5881 gaccatgagc aacgccctcg ccgcaaggct cggccacgcc cattccgtca aagacaacgc
     5941 ctggttcgtc ggcttcagcc cacgtcgcaa ccccgacatc gccgtctgcg tcctcttcga
     6001 agccggagag cacggcaagc tggctggccg tctcgcttcg cgcgtcatcg ccgcctatgt
     6061 cgacaagcag cgcacgctga accacaacct cattgcccag cagcccaaac cagtccaggt
     6121 gggcgcgatg tggtccgatc cggccaaccc gaacggaatg ggcggcaaac ccctgcctct
     6181 caagccggac cagaaatcca accccgagct ggcctccatt cgcgctggcc actacacggt
     6241 cccggcccag cccgccgatt cgcaatcgca cccgcgctga tcgtgacctt tccggatctc
     6301 ctcccgaacg acaaagcgat cgacccctgg tgtggtgtcc cggaagtaag caaagtaaat
     6361 ctgtcatccc gaccggagcg aagcgtagcg gagggacctg ctttttcttc catttcagac
     6421 gtcaccaatc actggagcaa gtaattggag ttatggcacg aactgcaggg accccacacg
     6481 agcagagcgg acctgcaact tgtatcccgc ccaatgcaca gacccggcta aaaggagaac
     6541 attcggcgaa cccagggccc gcgccgtccc cacctcgatt cgaactgccc gttgacagct
     6601 cactacccct tcccgctacc ggctgccggc ggctgttttg ctatactcaa acctgctgtc
     6661 aatctgtagt ttccgagcag gaccaaatgc ccaaactcaa aacgcatcgg ggcgcggcca
     6721 agcgcttcaa aaagaccggc acgggtaaaa tcaagcgcgg ccagtccaaa atgcggcata
     6781 tcctcacgtc caaggaagtc aaaaccaagc gccacctccg caagaacacg ctggtttctg
     6841 acgccgacta cgccaaggtc gcccgcatga ttccctacgc ctgatccttc cgggccaccc
     6901 acgcgggtgg ctcccttcgc gcgaatgcga gaagagagga caggcaaaac cgagcgagtg
     6961 aagaggcagt cccgcctcca gccgccttgc aaaacacaga acccgaaccc aaaggagact
     7021 ctccatgcca cgcgtcaaac gtggaaccaa acgatccgac cgccgcaaga aaatccttaa
     7081 aagggccagc ggctacttcc tcaccaaatc caagctctac caggcagccc aggaagccgt
     7141 cgagcgcgga ctgaagttcg cttactccgg ccgcaaaggc aagaagcgcc agtaccgctc
     7201 cctctggatc gtgcgcatca acgccgccgc caaagccaat ggcatctcgt actcgcagct
     7261 catcaacggc ctcaagaaag ccggcgtcga gctcgatcgc aagatcctgg ccgacctcgc
     7321 cgtgaacgat gcggccgcct tcaccgccct ctgcggtcag gccaaagccg cccaaaaacc
     7381 cgcagcagca taacgccggt atcacgcgcc tgaggggccc gtctgcacat cctgctgccg
     7441 acgggccttt cgctaaccgg ttcatcgctg accggttcat tgccgaccgc tctttagccg
     7501 accgttttta cctaacaggt tttcgctaac
//
LOCUS       DASZYN010000502         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1048607, whole genome shotgun sequence.
ACCESSION   DASZYN010000502 DASZYN010000000
VERSION     DASZYN010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048607"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VHE33_12980"
     CDS             complement(<1..367)
                     /locus_tag="VHE33_12980"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HVT98413.1"
                     /translation="MDRRFDVTVAGEINLDLILYGLPKDIPTERELLASDFRMTLGSS
                     SAIFAHNFAVLGNRVGFITRLGNDALGEIALSRLGEPGVDLSRAKRVQGGPSTGVTVI
                     LHHGSERRILTYLGTMAELT"
     gene            complement(499..>1509)
                     /locus_tag="VHE33_12985"
     CDS             complement(499..>1509)
                     /locus_tag="VHE33_12985"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HVT98414.1"
                     /translation="VEKPFANVLAEAKEARNAVKASRQVVQMGTQHRSQPYPLAVRDI
                     VRSGRIGQVIHIEQEWNVNEERWRFVEGDIGAPSEMLRDVSAEWKQWVNGHKSGLREE
                     DTDWKRWLLGKPDRPFDPHVYLEFRLYKEFSSGIFDQWLSHGCDLVHLWTDETHPVSV
                     VSNGGVFVWKDGRENPDTCVTAITYPKGFLYTYKTTFGNSYRSFSRIQGRDGTIENYG
                     GEGASLFVTTAEGGRKAHDSGPEGQVYTKAPGAGPAEDHPTQLTIPGAPPGSSAGPDD
                     DDAVHLLNWLRAMQDRREPNANVDHGFSHSIVCIMAAESYLSGKRLYWDPGSEEIREH
                     AV"
BASE COUNT          307 a          505 c          389 g          308 t
ORIGIN      
        1 ccgttagttc agccatcgtg ccgagatagg taagaattcg tcgctcgctg ccgtgatgca
       61 ggatcactgt gactccggtc gaaggtccgc cttgcacccg cttcgcccgg ctcaggtcca
      121 cacccggctc acccagtcgc gacagtgcaa tctcgccgag ggcgtcgttt ccgagccgcg
      181 tgataaagcc aacccgattc cccagcacgg caaaattatg cgcgaagatc gccgacgagc
      241 ttcccagcgt catccggaaa tcgctggcaa gcagctctcg ttccgtgggg atatctttcg
      301 gaaggccgta cagaatgaga tcaaggttga tctcaccggc aactgtcaca tcgaagcgcc
      361 tgtccacgat gcccgcaccc tcgcaatctg caaactccgc tcaaatttca agaaccccga
      421 caacacgcag catcccgaca aacaaaagca cactacgtag attaaggggt cacgtctgcg
      481 gatcgcatgc agcagacgtc acactgcatg ctcgcggatt tcctcgctcc caggatccca
      541 atagagccgc ttgccgctta agtaggactc ggctgccatg atgcacacaa tggagtgcga
      601 gaagccatga tccacgttcg cgttcggctc gcggcgatcc tgcatggcgc ggagccagtt
      661 cagcaggtga accgcgtcgt catcatccgg accggccgag cttcctggtg gcgcacccgg
      721 aatcgtcagc tgcgtgggat gatcttccgc cggaccggca cccggggctt tggtgtagac
      781 ctgcccctcg ggcccggaat cgtgtgcctt ccttccgccc tccgcggtcg tgacgaacag
      841 cgatgctccc tctccaccat agttttcgat cgttccatcg cgcccctgaa tgcgcgagaa
      901 gctccggtag ctgttcccaa aggtcgtctt atacgtatag aggaagccct ttggataagt
      961 aatcgccgtc acacaggtat ccggattctc tcgtccatct ttccacacaa aaacgccgcc
     1021 gttgctcacc acactgaccg gatgcgtctc atctgtccat aggtgaacaa ggtcgcagcc
     1081 atggctcagc cactggtcga atattcccga ggagaattct ttgtacagcc gaaactcgag
     1141 atacacgtgc ggatcaaaag gccggtccgg cttgcccagc agccagcgct tccagtctgt
     1201 atcttcctcg cgcaggcccg atttgtggcc attgacccac tgcttccatt ccgcgctgac
     1261 gtcgcgcagc atctcggacg gagcgccaat gtcgccctca acaaaccgcc agcgctcctc
     1321 attcacattc cactcctgct cgatatggat cacctggccg atgcggcccg aacgaacaat
     1381 gtcgcgcacc gccaatggat acggctggct gcggtgctgc gtgcccattt gcaccacctg
     1441 ccgcgacgcc ttaaccgcgt tgcgggcttc tttcgcctcc gccaacacat tcgcgaacgg
     1501 tttttcgac
//
LOCUS       DASZYN010000503         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_86371, whole genome shotgun sequence.
ACCESSION   DASZYN010000503 DASZYN010000000
VERSION     DASZYN010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86371"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VHE33_12990"
     CDS             complement(<1..528)
                     /locus_tag="VHE33_12990"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HVT98415.1"
                     /translation="MTTQQPFPPGYQPPPETITTSSQGSAYVPMDSWIYPAMDRLHSL
                     GYVDTAFMGIRPWTRLSIAHMLEQSADRIDTDTGNDEARKIYLAVLDEVQPDIDSATE
                     LNRPSGQLESVYTVLRGISGTPLRDSFHLGQSIINDYGRPYQGGFNNYTGFSVRAEAG
                     RFSFYYRGEYQHAPSA"
     gene            complement(772..>2190)
                     /locus_tag="VHE33_12995"
     CDS             complement(772..>2190)
                     /locus_tag="VHE33_12995"
                     /inference="COORDINATES: protein motif:HMM:NF036501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98416.1"
                     /translation="DALVDFIQRYDLPIPEADARAGKLYRPAADSPEMAYMHARRQGL
                     GGFMPKRDVPKIDLTAPKLETFAEWTAGSKGRAVSTTMGFVSMLRALMKDPKIGKLIV
                     PIVPDEGRTFGLESAIKQVGIYASEGQKYKPHDVDMLLSYREEKDGQILEEGITEAGS
                     MASFTAAGTGYSNYLVPTIPFYMYYSMFGFQRVGDMMWAFADARGKGFLMAGTAGRTT
                     MTGEGLQHQDGHSHLLMSVIPTCVSYDPAYVYELAVILQSGLKRMYENGEDVFYYITM
                     YNEDYAMPEMPKGVEEGIVRGIYKLKTGASGKVAAQLFGSGPILNEVLRAQQILAEKY
                     GVETDVWSVTSYNELRREALSVERWNRLHPAEAEKTPYIVSALSKSKGPVIAASDYMK
                     AVPDQLAPWLTTRLVSLGTDGFGRSDNREHLRRHFEVNAESVVAATLSKLARDGKFDA
                     KKAQKAMAELGVDAERGDPAHM"
BASE COUNT          394 a          775 c          557 g          464 t
ORIGIN      
        1 cgcgctcggt gcatgttgat actcgcctcg gtagtagaac gaaaaccgtc ccgcctccgc
       61 acgcacgctg aagcccgtgt agttattgaa tccgccctgg tacggccgtc cgtaatcgtt
      121 gatgatgctc tgtcccaggt gaaaactgtc tcgcaacggc gtcccgctga tcccgcgcag
      181 aaccgtgtag acgctctcta gttggccgct cggacgattc agctccgtcg cgctgtcgat
      241 atccggctgc acctcatcaa gcaccgcaag ataaatcttc cgcgcttcat cattccccgt
      301 atccgtgtcg atgcgatccg cgctttgctc cagcatgtgc gcaatgctca gccgcgtcca
      361 cggtcggatc cccataaatg ccgtatcgac atatcccagc gaatgcagac ggtccatcgc
      421 cggatagatc cagctgtcca tcggcacata ggccgatccc tgcgaacttg tcgtgatcgt
      481 ctcgggcggc ggctgatatc ccggtggaaa gggctgctgc gtcgtcacct gggctcttgc
      541 ggaaagaaga attgcgctgc ccaacaaaac tgcggataga aagaaagacg atctcaagcg
      601 gacctcggtg agattctgcc gttcaaggtg cgaccgtcaa taacaatggc gcctctgctt
      661 tcagactccg ggatcgaatt cctgctgtca agcgaactga gtgacactac tctgaacgcc
      721 atgtaaccgc agcgagtctg cgacctgtgc cctgcgacct gtgccctgtc ttcacatgtg
      781 cgccggatcg ccccgctccg catccacacc cagctccgcc atcgccttct gtgccttctt
      841 cgcgtcgaac ttcccgtcgc gcgccagctt cgacaaggtt gccgccacaa ccgactccgc
      901 attcacctca aagtgccgcc gcagatgctc gcggttatcg ctgcgtccga atccatccgt
      961 ccccagcgac accagccgcg ttgtcagcca tggcgccagc tgatccggca ccgctttcat
     1021 gtaatcgctg gccgcaatca ccggcccttt gctcttcgac agcgccgaga cgatgtacgg
     1081 cgtcttctct gcttccgccg gatgcagccg gttccagcgc tccacactca gagcctcgcg
     1141 ccgcagctcg ttatagctgg tcacgctcca cacatccgtt tccacgccgt acttctcggc
     1201 caggatctgc tgcgcgcgca gcacctcgtt cagaattggc ccgctcccga acagttgcgc
     1261 cgccaccttg ccgctcgccc cggtcttcag cttgtagatg ccgcgcacga tgccttcttc
     1321 aacgcccttc ggcatctccg gcatcgcgta gtcctcgttg tacatcgtga tgtagtagaa
     1381 gacgtcctcg ccgttctcgt acatccgttt caacccgctc tgcaggatca ccgccagctc
     1441 atagacgtaa gccgggtcgt agctcacgca cgtcggaatc acgctcatca gcagatggct
     1501 gtgcccgtcc tgatgctgca ggccctcgcc cgtcatcgtc gtgcgtcccg ccgtgcctgc
     1561 catcagaaat cccttgccgc gcgcgtcggc aaaggcccac atcatgtccc ccacccgctg
     1621 gaatccgaac atcgagtagt acatgtagaa cggaatcgtc ggcaccaggt agttcgaata
     1681 gcccgtgccc gccgccgtga atgacgccat cgatccggct tccgtgatgc cttcctccag
     1741 gatctgaccg tccttctctt cgcgatacga cagcagcatg tccacatcgt gcggcttgta
     1801 tttctgtcct tcgctcgcgt agatgcccac ctgcttgatg gccgactcca gcccaaacgt
     1861 tctgccctcg tccggcacaa tgggcacgat cagcttgccg atctttggat ccttcatcag
     1921 cgcgcgcagc atgctcacga atcccatcgt tgtcgaaacc gcgcgcccct tcgatccggc
     1981 agtccactcc gcaaacgttt ccagcttcgg cgcggtgaga tcgatcttcg gcacatcgcg
     2041 cttcggcata aagccaccca gcccctgccg ccgtgcatgc atgtacgcca tctccggcga
     2101 gtcggccgcc ggacgataca gcttgcccgc ccgcgcgtcc gcttccggaa tcggcaagtc
     2161 gtagcgctgg atgaagtcca ccagcgcatc
//
LOCUS       DASZYN010000504         1512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_926084, whole genome shotgun sequence.
ACCESSION   DASZYN010000504 DASZYN010000000
VERSION     DASZYN010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926084"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VHE33_13000"
     CDS             complement(<1..651)
                     /locus_tag="VHE33_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98417.1"
                     /translation="MSVVETASKIIGMEEGGDFPSTNWLHDPFTPLTTILTNDEQRQA
                     LLDLLDQLWPPQNPAGVAANEKWHPILGPQADGNLYFTVANGTGPLTLGLAGERHTSG
                     GGVGASLRARVPVVRIDGEAVTFVAGSSDGPLQVDLRVELNWSASGGHAIGLRAIHAT
                     ASLQPLSTPPANLTIVLEGLSLNGAAPADTVLDASKLDSQALQLVMGLLQEELQQLS"
     gene            complement(648..>1512)
                     /locus_tag="VHE33_13005"
     CDS             complement(648..>1512)
                     /locus_tag="VHE33_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98418.1"
                     /translation="VPFTFTIFQRALRHSGVLQPMGKNVSLRLVFSTDEKPAAGGHLQ
                     LRLHNPSTGECRELQHDPSVPLLIEDWLAEAGHDCRLEIASPWFKSESRWFSVLHDAL
                     TEIEIVLHRCADEEKRCTCPPPKIADRMDEKSLTQHLAIRLAGTPADGTAAPQQSSRQ
                     VIWVDGGDEVLVHLDSVRTAILDGLILVSIDLETDQTGRTSMVVPFAVGGANDPAGLV
                     AVTEDFPSGNGVLASRWGRAVQTAAWSSLLSLASEHAAERASAPLGISAVKGTVALTA
                     GAALHVGGTVR"
BASE COUNT          294 a          481 c          460 g          277 t
ORIGIN      
        1 gctgagctgc tgcagttctt cctgcaacag ccccatgacc agctgcaatg cctgcgagtc
       61 cagtttcgac gcatccagaa ctgtgtcggc gggtgctgcg ccgttgaggc tcaggccttc
      121 caggacaatc gtcagattgg caggcggcgt ggacaacggc tgcaggctcg ccgtcgcgtg
      181 aatcgcccgc aaaccaatgg catgtccacc ggaagcgctc cagttgagct cgacgcgcaa
      241 atccacctgc agcggcccat cgctgcttcc ggcaacaaag gtgacggctt cgccatcgat
      301 gcgcaccact ggaacgcggg cgcgcagcga cgctcccacg ccgcctccgc tggtgtgtct
      361 ctcacctgcc aggccaagcg tcagcggacc agtaccattc gcaacggtga agtagagatt
      421 gccgtccgcc tgcggcccca gaatcggatg ccacttctcg ttcgccgcta cgccagccgg
      481 attctgtgga ggccacagct ggtcgagtag atcaaggaga gcctggcgct gctcgtcgtt
      541 ggtgaggatc gtcgtgagtg gtgtgaaagg atcgtgcagc caattcgttg aagggaaatc
      601 tcctccctcc tccatgccga tgattttcga ggcagtttca acgacactca tcgcacggtc
      661 cccccgacat ggagtgccgc gcccgcggtg agcgcgaccg tacccttcac ggccgaaatg
      721 cccagcggag cggaggcacg ctcagcagcg tgctcgctgg cgagagaaag caggctggac
      781 cacgccgctg tttgcacagc gcgtccccag cgcgacgcca aaactccgtt gccgctcggg
      841 aagtcttccg tgacggcaac cagacctgca gggtcgttcg cgcccccgac cgcgaagggg
      901 accaccatcg acgtgcgtcc ggtttggtca gtctcaaggt cgatagaaac cagaatcagg
      961 ccatcgagga tcgccgtgcg aaccgaatcc aggtggacca gcacctcgtc gccgccgtcc
     1021 acccagatca cttgcctgga ggattgctgc ggcgccgcgg ttccatcggc tggcgttccg
     1081 gcaagacgga tcgccagatg ctgtgtcaga cttttctcat ccattcggtc cgcgatcttc
     1141 ggcggcggac aggtgcaacg cttctcctcg tcagcgcacc gatggaggac gatctcgatc
     1201 tcggtcaatg catcgtggag aacagagaac catcggctct ctgacttgaa ccacggcgag
     1261 gcgatctcga gtctgcaatc gtgacccgct tccgcgagcc aatcttcgat cagcagcgga
     1321 acggacggat cgtgttgcag ttctctgcat tcgcctgtcg agggattgtg cagccggagc
     1381 tgaagatgcc cgcccgcagc cggtttctca tccgtcgaaa agacgagccg taatgaaacg
     1441 ttcttgccca taggctggag aaccccgctg tggcgaagag cgcgctggaa gatagtgaag
     1501 gtgaagggta cg
//
LOCUS       DASZYN010000505         2359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_926120, whole genome shotgun sequence.
ACCESSION   DASZYN010000505 DASZYN010000000
VERSION     DASZYN010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926120"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VHE33_13010"
     CDS             <1..679
                     /locus_tag="VHE33_13010"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HVT98419.1"
                     /translation="AYVLSVGERASGQELTRSLLEKIASFAFCSRAGMHYTARKQKAR
                     RGGDYWYAYRRLRGRLVKRYLGKTEDLTLARLEEVARGLTEMSDARESAVPASLETLL
                     PLSGKSVVDAAHPETARRSFAMETLLLSRLSPPRLLTYLRSVLRVLPAHAPDGTPASP
                     LLEPLSPQEKRVLRLLAEHRSNAEIARELVVSVNTVRTQVQSIYGKLGVHKRSAASDV
                     ARELRLF"
     gene            complement(699..1667)
                     /locus_tag="VHE33_13015"
     CDS             complement(699..1667)
                     /locus_tag="VHE33_13015"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT98420.1"
                     /translation="MPLDPQVALYLQQQAEMSMEPGPELSLAEQRRQAELVALEQAGT
                     PAAVAALVDRVIPGPGGDIPLRIYTPEGAGPFPVLVYFHPGGWVFGSIAGSDPVCRAI
                     ARQTPCLVVSVGYRLAPEHPFPAAPEDCYAAIKWVAEHAGDIQADAGRIAVGGESAGG
                     NLAAAVSLMARDRQGPALCLQVMIYGETAYYEPGTASYRTYAQGYNLTRDDMIWFWDQ
                     YLARKEDATHHYASPLYAASLSQLPPALIITAEYDPVRDEAEEYARRLQEAGVAVQLS
                     RYQGMVHSFFRMFAVFERSKVALCEVTTALAAAFAPVQDEHASPGR"
     gene            complement(1888..2028)
                     /locus_tag="VHE33_13020"
     CDS             complement(1888..2028)
                     /locus_tag="VHE33_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98421.1"
                     /translation="MNNAAQWFAVQELHIAIPVLVLIQQQVKTFAFENRGDVEDNFNG
                     LL"
BASE COUNT          474 a          709 c          691 g          485 t
ORIGIN      
        1 ggcttatgtc ctttccgtgg gtgagagggc cagcggccag gagttgacgc gctctttgct
       61 tgagaagatc gcttcattcg ccttctgtag tcgcgcgggg atgcactata ctgcccgcaa
      121 gcagaaggcc aggcgcgggg gcgattactg gtacgcctat cgccgcttgc ggggacggct
      181 ggtcaaacgc tatctgggca aaacggagga tctcaccctg gcgcgcctgg aggaggttgc
      241 gcgcgggctg actgagatga gcgatgcgcg cgagtccgct gtgccggcgt ccctggagac
      301 tcttctgcct ctctctggaa agtctgtggt ggatgcggct cacccggaga cggcgcggcg
      361 ctcttttgcg atggagacgc tattgctctc ccgactgtct ccgccgcgtc tgctcacgta
      421 cctgcgctcg gtcctgcgtg tcttgcccgc gcacgcgccg gacggtacgc cggcctcgcc
      481 tctgcttgag ccgctttcgc cccaggaaaa gcgcgtctta cgcttgctgg ccgagcaccg
      541 ctccaacgcc gaaatcgccc gcgaactggt tgtctcggtc aacacagtgc gcacgcaggt
      601 ccagagcatt tatggcaagc tcggcgttca caaacgcagc gccgccagtg atgttgcccg
      661 cgaactgcgc ctgttctgag tcgcgttcag acgatgcttc accgtccagg gctggcgtgc
      721 tcatcctgaa cgggggcgaa ggcggcagcc agggccgttg tgacctcgca gagcgcgact
      781 ttgctgcgct cgaaaaccgc gaacatgcgg aagaaactgt ggaccatgcc ctgatagcgt
      841 gaaagctgaa ccgccacacc cgcctcctgg agacgccggg catactcttc cgcctcatcg
      901 cgtaccgggt catattcggc ggtgatgatg agcgcgggcg ggagttgact caggctggcc
      961 gcgtacagcg gagaggcata gtgatgagta gcgtcctcct tgcgcgcgag atactgatcc
     1021 cagaaccaga tcatatcgtc ccgtgtaagg ttatagccct gcgcgtaggt cctgtacgag
     1081 gccgttcccg gctcatagta agcggtttcg ccgtagatca tgacttgcag gcagagagcc
     1141 ggtccctggc gatcccgcgc catcagggac accgccgccg ctaaatttcc tcccgcactc
     1201 tcgcctccca ccgcaatgcg tccggcatcg gcctgaatgt ccccggcgtg ttccgccacc
     1261 cattttatcg cggcatagca atcttccggc gcggcgggaa aaggatgttc cggcgccagc
     1321 ctgtagccca cggagacgac caggcagggc gtctgcctgg cgatagcccg gcaaaccggg
     1381 tcgctgcccg cgatgctgcc gaaaacccag ccgccgggat gaaaatagac cagtacagga
     1441 aacggtccgg cgccctccgg ggtatagatc cgcaggggaa tatccccgcc tggaccggga
     1501 atcacgcggt ccaccagcgc ggcaacggct gcgggcgtgc ccgcctgctc aagcgcaacc
     1561 agttccgcct ggcggcgctg ctccgccagc gaaagctccg gtccaggctc catgctcatt
     1621 tctgcctgtt gctgcaaata aagggcgacc tgtggatcaa gcggcatatg tgatacttcc
     1681 tttcatgact tgccggggaa agaagagaaa gaatggaaat aaagtctcca gggcagcggc
     1741 gcctcagtcg tctgctcaaa tgatatcatc caccagatca tgcgcgcaat catcccaacg
     1801 gatgaagtcc ggggaacccc cggcggacta tacttcggac atcgaacgca cagcaaagac
     1861 attcgcgtag agcaaaatta atcttcttta taaaagacca ttgaagttat cttcaacatc
     1921 gccccggttc tcgaaggcaa aggtcttaac ctgctgctgg atcagcacga gtaccggtat
     1981 cgcgatgtgc aactcctgga ctgcaaacca ttgggcggcg ttgttcaact gcgctacgcc
     2041 cttgacccac agtgagcagg accatcaagg gcacaaccct tgatccgttt acctacccac
     2101 tcgtcttttg ttatcggtca tcccgaatgt ttccaacgtg cccctgccgc attggaaaca
     2161 ttcgatcccc ttggaaaagt cagctggact gatgatgcta taattctggg catgagcaac
     2221 gttttttctt agatgggtct gttctgtagg tggaaatact gagttgggaa agatacccgc
     2281 cagactgtga tgaaagcact aatcggttgg tgcatctcta tgttatagta taggtgaatt
     2341 cattgttgtg atcggagag
//
LOCUS       DASZYN010000506         2044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_666295, whole genome shotgun sequence.
ACCESSION   DASZYN010000506 DASZYN010000000
VERSION     DASZYN010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666295"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VHE33_13025"
     CDS             <1..1099
                     /locus_tag="VHE33_13025"
                     /inference="COORDINATES: protein motif:HMM:NF037199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HVT98422.1"
                     /translation="TLMRHLKQIDDEQHTVIAVQVENESGNIGSVRDNSPEANKLFAG
                     PVPSDMLQASGKSAGAWQQVFGSDADELFQAYYQAKYINTIATAGKNEFAIPLYINVW
                     IDYPGAALPQRQLEEPGIAYPSGGAVQKLVGLWRKLAPAIDMIGPDIYTDDSQFYRET
                     MDAYQRPDNPLWIPETGRSDSFAKFFFYALGRGAIGFSPFGVDKSGWNILGDEPWSAH
                     ARNFALISPMDRTIAQLEFDGKVKTAVEEPGQPTQEIDFGAWQATIGFGFPQHDGRRA
                     PGTKDFHGAALVAQLGPDEFLITGVDASIVFHLPGKLPWIHSQILTAEEGTYENGVWK
                     PQRILNGDETDRGLCFYTKPTTAVRITMQKF"
     gene            complement(1329..1727)
                     /locus_tag="VHE33_13030"
     CDS             complement(1329..1727)
                     /locus_tag="VHE33_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98423.1"
                     /translation="MYVVDQASLQRIIKAPMSGSLVRIDPSAQIILSADHKAEFSHVP
                     ADLIDGSLCSVTGRGTWRLGKNNRYITVLVQIADEEPNNRCKDTFTAKFAEELNLYGK
                     KPPYKLHVTIGDPDSGDAVQFERRFICPRC"
     gene            complement(1844..>2044)
                     /locus_tag="VHE33_13035"
     CDS             complement(1844..>2044)
                     /locus_tag="VHE33_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98424.1"
                     /translation="TPPSRWHFVAREEMPARFGTEARIIFGSLRTAEAVPFQTQPGMP
                     SRRCTGALEDFVRIFDHGTVVP"
BASE COUNT          444 a          706 c          532 g          362 t
ORIGIN      
        1 cacgctcatg cgtcacctca aacaaatcga cgacgagcag cacaccgtca tcgccgtcca
       61 ggtcgaaaac gaatccggca acatcggcag cgtccgcgac aactcccccg aagccaacaa
      121 gctcttcgcc ggccccgttc cctccgacat gctccaggca tccggtaaat ccgccggcgc
      181 ctggcaacag gtcttcggca gcgatgccga cgagctcttc caggcctact accaggcgaa
      241 gtacatcaac accatcgcca ccgccggcaa aaatgaattc gccatcccgc tctacatcaa
      301 tgtctggatc gactatcccg gtgcggccct gccccagcgc cagctcgaag agcccggcat
      361 cgcttacccc agcggcggcg cggtgcaaaa gctcgtcggc ctctggcgca aactcgctcc
      421 cgcgatcgac atgatcggcc ccgacatcta caccgacgac tcccagttct accgcgagac
      481 catggacgct tatcagcgcc ccgacaatcc gctctggatc cccgaaaccg gccgcagcga
      541 cagcttcgct aaattcttct tctacgccct cggccgcggc gccatcggtt tctctccctt
      601 cggcgtcgac aagtccggct ggaacatcct cggcgacgag ccctggagcg cgcacgcccg
      661 caacttcgcc ctcatcagcc ccatggatcg caccatcgcc cagctcgagt tcgacggcaa
      721 agtcaaaacc gccgtcgaag agccaggcca gcccacccag gaaatcgact tcggcgcctg
      781 gcaagccacc atcggcttcg gctttccgca acacgatgga cgccgcgccc ccggcaccaa
      841 agacttccac ggcgccgccc tcgtcgccca gctcggcccc gatgaattcc tcatcaccgg
      901 cgtcgacgcc agcatcgtct tccacctgcc cggcaagctg ccctggattc acagccagat
      961 tctcaccgcc gaagaaggca cctacgaaaa cggcgtctgg aaaccgcagc gcatcctcaa
     1021 cggcgacgag accgatcgcg gcctctgctt ctacaccaaa cccacgacgg cggtgcgcat
     1081 caccatgcag aagttctagt gcggggataa tttctcatcc caccggcctg ggcacgactc
     1141 aacgggtgcc ccacatctgc ccgctgttgg cagatgtggg aacatcggat ccgaaccggc
     1201 cgccctgatt cattcgtccc aacaaagcag gcgcgagatt catctcgcgc aaacaccact
     1261 cggccaaaaa ctcgggagct tcagccccga aacccgctct tgcggctaac tttgacagaa
     1321 ccggtaattc agcagcgagg gcaaatgaat cgccgttcaa actgcacggc gtctcccgaa
     1381 tctgggtcac caatcgttac gtggagcttg taaggcggct tcttgccgta caggttgagt
     1441 tcttccgcaa atttcgcggt aaaggtatcc ttgcagcgat tgttcggttc ctcatcggca
     1501 atctgcacca ggacggtgat gtatctattg ttcttcccca ggcgccaagt gccgcgtcct
     1561 gtgactgagc acaatgaacc atcaatgagg tctgctggta cgtgagagaa ctcggccttg
     1621 tgatctgcgg acaggataat ttgcgctgag ggatcgatcc tgaccagcga tccgctcatc
     1681 ggcgccttga taattcgctg caacgaagcc tgatcgacaa cgtacaaacc cgcgacatcc
     1741 tgttcggcgg gttgctgcgt aaggaatcta cctgcccagg gatcgtattg acagcctgcg
     1801 agagtcacaa ttgcgaggat cgcaaataag gatggtcggc atctcacgga accactgtac
     1861 cgtggtcgaa gatgcgaacg aagtcctcaa gggctccggt gcaccgacga ctcggcatcc
     1921 ccggctgggt ttgaaagggc acggcttcag cggtgcgcaa gctcccgaaa atgatccggg
     1981 cttcagtccc gaaacgcgcc ggcatttcct cgcgagcgac gaaatgccaa cgcgagggcg
     2041 gggt
//
LOCUS       DASZYN010000507         5390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_926259, whole genome shotgun sequence.
ACCESSION   DASZYN010000507 DASZYN010000000
VERSION     DASZYN010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926259"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VHE33_13040"
     CDS             complement(<1..252)
                     /locus_tag="VHE33_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HVT98425.1"
                     /translation="MVSRRGFLRVVGGAAAVGAAGSVSGWGEFRGDGEEDSSLPAPIA
                     ALPSFTGKVAPISNDERRVRIERAKQLMAENKLDAIVLAN"
     gene            472..1218
                     /locus_tag="VHE33_13045"
     CDS             472..1218
                     /locus_tag="VHE33_13045"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HVT98426.1"
                     /translation="MSGHSKWATIKHKKGALDAKRGKIFTQLIKEITIAAKSGGDPDG
                     NPRLRSAIAAAKAENMPNDNIKRAIQRGTGELPGAVYEEIAFEGYGPGGVALIVEATT
                     DNRNRAVSEIRHTFSKHGGNLGEPNSVRFMFSKKGIIAVAKDQADEEKLMNIVLEAGG
                     EDLNDEGDTWEIITEPSAYEAVAQAVRDAKIETVMSEITMIASTYTRLEGPQAAQMVR
                     LIEALEDHDDVNHVYSNFDMDAKQLEEVTS"
     gene            1314..2036
                     /locus_tag="VHE33_13050"
     CDS             1314..2036
                     /locus_tag="VHE33_13050"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HVT98427.1"
                     /translation="MKARIFLLAFAAMLTAAIPALAQSPAADADAPAAAAFAGRPWEY
                     GPFFQGGMGIGDRDQYKFTAAGFRLGKVITGEHLSGVFRGQFELAGEIMPWWQAFTPA
                     PHLEQGTCGDNATGLTYPCQLAVGGGTFSGASLTPVILRWDFKPGRRIAPWFQAAGGL
                     IYTTHKFPPDVLVPHGLPGGTSVWNFSPQGGIGIHYFIRPGRAITLSANGVHISSASL
                     GDRNPGVNASVSFQIGYTFFGR"
     gene            2114..3049
                     /gene="ftcD"
                     /locus_tag="VHE33_13055"
     CDS             2114..3049
                     /gene="ftcD"
                     /locus_tag="VHE33_13055"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HVT98428.1"
                     /translation="MPDPLIECVPNFSEGRRPEVIEAIVSSMHVEGVRLLDYSLDADH
                     NRSVVTIAGAPAAVVESAVRAAGRASELIDLTTQEGVHPRIGAADVIPFVPVRDVSLE
                     QCVILARQAGNEIWNRHQVPVYFYEAAAARPDRVMLEEVRRGQFEGLRDAVHREPARR
                     PDVGGPSLHPTAGASAVGARRFLIAFNLYLDTAEVAVARAIAREIRQSGGGLAGVKAR
                     GVSAHGQAQVSMNITDFTRTPVGQVFNAVKEKARKHGAAPASAELIGLLPEAALERNS
                     EWLRLLGDGGFDPEEKVLERRLEHPLAWPAGSEQR"
     gene            3201..4157
                     /locus_tag="VHE33_13060"
     CDS             3201..4157
                     /locus_tag="VHE33_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98429.1"
                     /translation="MKFGKHLVFALAMAAVVAVALPARAAQLDTDARSAIPHDVQQLI
                     VVDYGVMENSSAAMDLKARILPPELKQLESALKTSGLDENHDIDQLAFAAYRVAGSDN
                     VRIVGLAQGQFQVSEIMATLQKKKVKATAVRNNKLYPMGASGMLVTFLNPTTMLFGSS
                     DDIKPALDCRDGLAPSFLTNQDMLAAMQKVESAPIWSILDQKGTQYMMHSLLGQASQL
                     GDYETVRKRLLSSSYTMDFNNGVKFNLYVLTSDTFTAATMSSLLNAAAMYEKVSGNPM
                     EKQAIDATTIDSSAGTLSVRFAASDSQFSALLQSPLFQNVVR"
     gene            4305..>5390
                     /locus_tag="VHE33_13065"
     CDS             4305..>5390
                     /locus_tag="VHE33_13065"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HVT98430.1"
                     /translation="MRKSTPSLQLVLAGIVTASVLTLAAALAATSGRKGNAAASYSQA
                     TAANAGILSSAPLQVDRRDPSAGRIVPADARLERLADGFKWVEGPIWTQGNLFFAEIP
                     SNSIRRWIPGTGVSIFIHPSGYRGAEPYGGPESGSNGMTLDARGRLTIAGHARRNIYR
                     LESLDPKAQITVLADAYQGKKLNSPNDLVYRSDGSLYFTDPPYGLRTQKDTDPEKQLK
                     VNGVYRIPHALSQKPGAPPANGDLQLLVKDLPRPNGIAFSPDEKYLYVSNSEPRKIWM
                     RYRVQPDGTVTDAKLLYDATADERVGGPDGMKVDREGDIYSAGPGGVTILSPDGKLLA
                     TLLIPERVSNVAWGGADRTTLYITASSS"
BASE COUNT         1073 a         1815 c         1467 g         1035 t
ORIGIN      
        1 gttcgcaagc acgatggcat caagcttgtt ttctgccatt agctgtttcg cgcgctcgat
       61 tcgcacacgg cgctcgtcgt tcgaaatggg tgcaactttt ccggtgaaag agggcaacgc
      121 cgcaatcgga gcgggaaggg agctgtcctc ctcgccatcc ccccggaact caccccatcc
      181 cgaaaccgag cccgctgccc ccactgcagc cgcaccaccc acgactcgca gaaaccctcg
      241 cctcgaaacc attcgctctc ctcatgacag ctttgcggaa tccgcaaatt gtaactgttc
      301 gttcgttatg cgctcgacgc cttctcgcta tccgttgtac ttacgctggc cgctacccgc
      361 tatgcgctgc ctcctgctat actcaaccgt tgcgggcaaa ccccggaaac tgctgtagtt
      421 ccggcctttc gccccagaaa ttccatccaa aatcagactt ctgaggtttc catgtccggc
      481 cattccaaat gggcgacaat caagcacaaa aagggtgcgc tcgacgctaa acgcggcaag
      541 attttcacgc aactgattaa ggaaattacc atcgccgcga aatctggcgg cgaccccgac
      601 ggcaatcctc gcctgcgcag cgccatcgcc gctgccaaag ccgagaacat gcccaatgac
      661 aacatcaagc gtgccatcca gcgcggcacc ggcgaacttc caggcgccgt ctatgaggaa
      721 atcgccttcg agggctacgg cccgggaggc gttgcgctca tcgtcgaagc caccacggat
      781 aaccgcaacc gcgcggtcag cgaaatccgc cacaccttct caaaacacgg tggcaacctc
      841 ggcgagccga attcggtccg attcatgttc tccaagaagg gcatcatcgc cgtcgccaaa
      901 gaccaggccg acgaagagaa gctcatgaac atcgtgctcg aagccggagg cgaggacctc
      961 aacgacgaag gcgacacgtg ggagatcatt accgagccca gcgcgtatga agctgtcgcg
     1021 caggccgtgc gcgacgcgaa gatcgagacc gtcatgtccg agatcacgat gatcgcctcc
     1081 acctacacca ggctcgaagg tcctcaggcc gcacagatgg tacgcctgat cgaagctctc
     1141 gaagaccacg acgacgtcaa ccacgtctac tccaatttcg acatggacgc gaagcaattg
     1201 gaagaagtga ccagctagcg gccgtaatct cattcaccga gggggccgcc tgctggcggc
     1261 ccttcccttg atctgaagtc cggcccggag cctgatcggg agtcgatcgt cgagtgaaag
     1321 cacgcatctt tcttcttgcc ttcgcagcca tgttgacggc tgccatcccg gccctcgccc
     1381 agtcgcctgc agcggacgcc gatgcacccg ctgctgcagc gttcgccggc cgcccctggg
     1441 aatacggtcc cttctttcag ggcggcatgg gtattggcga ccgcgaccag tacaaattca
     1501 ccgccgccgg tttccgcctc ggcaaagtca tcaccgggga gcatctgagc ggcgtcttcc
     1561 gtggccaatt cgaactcgca ggcgaaatca tgccctggtg gcaggccttc actcccgctc
     1621 cgcatctcga acagggaacg tgcggcgata acgccacagg cctcacctac ccctgccaac
     1681 tcgcagtcgg tggtggaacc ttcagcggcg ccagcctgac ccccgtgatc cttcgctggg
     1741 acttcaagcc cggccgtcgc atcgcgccct ggttccaggc cgccggcggg ctgatctaca
     1801 cgacccacaa attccccccc gacgtcctcg ttcctcacgg tctgcccggc ggcaccagcg
     1861 tttggaactt ttcgcctcag ggcggcatcg ggattcacta tttcatccgg ccgggtcgcg
     1921 ccatcacgtt atccgcgaat ggcgtccaca tctccagcgc ttcgctcggc gatcgcaatc
     1981 ccggcgtcaa tgccagcgtc agtttccaga tcggttatac gtttttcggc agatagtttt
     2041 acaacggaca agcgaagcat ccgtcaatcc gcgtatcatc ccacgttgcg ttctccacga
     2101 gactctgatc aagatgcccg atcccctcat cgaatgtgtt cctaatttct ctgagggccg
     2161 ccgtcccgaa gtgatcgaag cgattgtctc gtctatgcat gtcgaaggcg tgcgcctcct
     2221 cgattactcc ctcgacgccg atcacaaccg ctccgttgtc accattgccg gtgcccctgc
     2281 cgccgtggtc gaatccgccg tgcgtgccgc cggccgtgcc tccgaactca tcgatctcac
     2341 cacccaggag ggcgtccacc ctcgcatcgg cgcggccgac gtgatcccct ttgttccggt
     2401 tcgtgacgtc tcgctggagc agtgcgtgat ccttgccagg caggcaggca acgaaatctg
     2461 gaaccgccat caggttccgg tctacttcta tgaagccgca gccgcgcgtc ccgatcgcgt
     2521 catgctcgaa gaggttcggc gcggacaatt tgaaggtctt cgcgatgctg tccaccggga
     2581 accggcccga cgccctgacg tcggcggtcc ttcgttgcat ccgacggccg gtgcctccgc
     2641 cgtcggcgcg cgccgcttcc tcatcgcctt caatttgtat cttgataccg cggaggtcgc
     2701 ggtggcccgc gcgatcgcca gggaaatccg ccagtccggc ggcggcctgg ccggcgtcaa
     2761 agcccgcggc gtttccgcgc acggccaggc ccaggtttcg atgaacatca ctgactttac
     2821 ccgcaccccg gtcggtcagg tcttcaacgc ggtgaaggaa aaggcccgaa agcatggtgc
     2881 tgcgccggcc agcgcagaac tcatcgggct ccttcccgag gctgctcttg agcgaaacag
     2941 cgaatggctg cgtctgctgg gtgacggagg gttcgacccg gaggaaaagg ttctggagcg
     3001 gcgccttgag cacccactgg catggcctgc aggtagtgaa caacgatagt tgtaggccga
     3061 cgtcggactc aaccagcgta aaattgacat acagtgcggc ccaggcgtgt cctttcgcat
     3121 ccacctgagg tcaaagacca cagcggtctt cagattccct atactcagcc ggctatccgg
     3181 cggaggaagc aggtttatcc gtgaaattcg gtaagcacct cgtctttgct ttagctatgg
     3241 cagcggtagt cgccgttgcg cttcctgcgc gtgctgcgca actcgatacc gatgcccggt
     3301 cggccatccc ccacgatgtc cagcagctca tcgtcgtcga ctacggtgtg atggagaatt
     3361 cctccgcagc catggatctc aaggcgcgga tcctgccacc tgaactgaag cagctggaaa
     3421 gtgcactcaa gacctccggc ctcgatgaga atcacgacat cgaccagctt gcctttgccg
     3481 cctaccgtgt cgctggcagt gataatgtcc gcatcgtcgg actcgcgcag ggtcagttcc
     3541 aggtcagcga gatcatggct accctgcaga agaagaaggt aaaggcgacg gcggtccgca
     3601 ataacaagct ctatcccatg ggcgcaagcg gaatgctggt cacgttcctg aatcccacca
     3661 ccatgctgtt tggcagcagc gatgacatca agcccgcgct cgactgccgg gatggcctgg
     3721 ctcccagctt ccttaccaac caggacatgc tggctgcgat gcagaaggtt gaatccgccc
     3781 ccatctggag catcctcgat cagaagggca cgcagtacat gatgcactcg ctcctcggcc
     3841 aggcatcgca actcggagac tacgagaccg tccgcaaacg cctgctcagc tccagctaca
     3901 ccatggactt caacaacggc gttaagttca atctgtatgt cctgacttcc gataccttca
     3961 ccgctgcaac gatgtcctct ctgctcaatg ctgccgcgat gtatgagaaa gtcagtggca
     4021 atccgatgga aaagcaggcc atcgacgcta ccaccattga ctcctccgcc ggcaccctca
     4081 gcgtcaggtt cgcggcctcc gacagtcagt tctccgcgct cctgcagtcg cctctgttcc
     4141 aaaacgtcgt gcgctgaggg ctggccgtcc cggcggatca ccagtcccga tgggatcatg
     4201 cattagaaaa ggagcagcct ctttggctgc tcctttctgt ttcccgcgaa ggcgcaacga
     4261 gcggtacact cgattccgcg cgcatctcga caaaggaagg cttcatgagg aagagcacac
     4321 cgtcactcca gcttgtgctg gcaggaatcg tcactgcatc cgtgttgacc cttgcggccg
     4381 ccttagccgc cacctcgggc agaaaaggga acgctgctgc ctcttatagt caagccaccg
     4441 ctgcaaacgc cggcatcctc agttccgccc cgctgcaggt ggatcgccgt gatccttccg
     4501 ctggccgcat cgttcccgct gacgctcgtc tcgagcgcct cgccgacggc ttcaagtggg
     4561 tcgaaggtcc gatctggacg cagggcaatc tcttctttgc agaaattccc agcaatagca
     4621 tacgacgctg gatcccgggt actggcgtga gtatctttat ccatcccagt ggctaccgcg
     4681 gggccgagcc ctacggcggt ccggaatcgg gctccaacgg catgaccctc gacgcgcgcg
     4741 gccgcctcac catcgccggc catgcccgcc gcaatatcta tcgccttgaa tcgctcgatc
     4801 ccaaagcgca aatcaccgtc ctcgccgacg cgtatcaggg caaaaagctc aacagcccca
     4861 acgatctcgt ctaccgctcc gacggctctc tctacttcac cgatcctccc tacggtttgc
     4921 gcacgcaaaa ggacaccgat cccgaaaagc agttgaaggt gaacggcgtc tatcgcatcc
     4981 cgcatgcgct cagccaaaag cccggcgcac cgccggccaa cggcgatctc cagttgctcg
     5041 tgaaggacct tccgcgcccc aatggcattg ccttttcacc cgacgagaaa tacctctacg
     5101 tcagcaattc ggagccccgg aaaatctgga tgcgctatcg cgtgcaaccc gacggcaccg
     5161 tgacggatgc caaactcctc tatgacgcca cggcggacga gcgcgtcggc ggccccgacg
     5221 gcatgaaggt ggatcgcgaa ggcgatattt atagcgccgg acccggcggc gttaccatcc
     5281 tttcgcccga cgggaagctt ctggcgacgc tgctcattcc tgagcgggtc tcgaacgtag
     5341 cgtggggtgg cgccgatcgg acgacgctct atatcacggc cagcagcagt
//
LOCUS       DASZYN010000508         6646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1243873, whole genome shotgun sequence.
ACCESSION   DASZYN010000508 DASZYN010000000
VERSION     DASZYN010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6646
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243873"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VHE33_13070"
     CDS             complement(<1..461)
                     /locus_tag="VHE33_13070"
                     /inference="COORDINATES: protein motif:HMM:NF014699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HVT98431.1"
                     /translation="MAATTSFAHEERLITPTGSSHPGPTVRSSLLVAVRYTLVTTVLL
                     GIIYPLVVTGIAQLTMRDKANGQLITRNGEIIGSAIIGQGFTSPRYFHSRPSAAGNGW
                     DATSSGGSNLAQSNKALQTRIETDVANWQKQNPGQPVPIGLVTTSASGLDP"
     gene            complement(479..2509)
                     /gene="kdpB"
                     /locus_tag="VHE33_13075"
     CDS             complement(479..2509)
                     /gene="kdpB"
                     /locus_tag="VHE33_13075"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HVT98432.1"
                     /translation="MAKKQNLWNSEILRQAVIDSFRKLDPRVMMKNPVMFVVEVGSVV
                     TTALLIEESIRHLPIRFDLQITLWLWFTVLFANFAEAIAEGRGKAQADTLRKAKGETM
                     AWRFRPDGSTEAVSSSQLRADDIIRVEAGQFIAGDGEVIEGVASVDESAITGESAPVI
                     REAGGDRSAVTGGTRVLSDWIKIRITSNPGETFLDRMIALVEGATRQKTPNEIALSIL
                     LAGLTVIFLLAVATLQPYAIYSHAPQTIFVLVSLLVCLIPTTIGALLSAIGIAGMDRL
                     VQYNVLAMSGRAVEAAGDVNTLLLDKTGTITLGNRQATEFFPAPGITTERLADVAQLA
                     SLSDETPEGRSIVVLAKDKYNLRGRDVSGMRAEFVPFSAQTRMSGVDLPGSKIRKGSA
                     DAIARWLESQGSSLPRPVSDRVDEIARQGGTPLVVSENATALGVVYLKDIVKGGLKDR
                     LARLRAMGIRSIMITGDNPLTAAVIAREAGVDDFLAEAKPKDKMDLIKREQARGKLVA
                     MTGDGTNDAPALAQADVGVAMNTGTQAAKEAGNMVDLDSNPTKLIEIVEIGKQLLMTR
                     GALTTFSIANDVAKYFAIIPAMFAGVFPVLNALNIMHLGNPHSAILSAVIFNAIIIIA
                     LIPLALKGVKYEPMSAQALLRRNLVLYGLGGIIVPFIGIKIIDLAIIGLHLA"
     gene            complement(2524..4233)
                     /gene="kdpA"
                     /locus_tag="VHE33_13080"
     CDS             complement(2524..4233)
                     /gene="kdpA"
                     /locus_tag="VHE33_13080"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HVT98433.1"
                     /translation="MTFNGWLQFLVYFVVLLVCMRPLGIYMNGVFSGKYKFLGPLENL
                     IYRASGIRGEEMTWGQYTAAMLLFSFVSLLLTYLIERLQLHLPWNPQHFPGVEQFLAW
                     NTAASFTTNTNWQAYTGEAVMSYFTQMAALAYHNFFSAAVGIAVAIALVRGIARRESK
                     TIGNFWVDTTRACLYVLLPVCIVAALVLVSQGVIQNLHPYTVAHTLEHQTQTIAQGPV
                     ASQEAIKELGTNGGGFFNANSSHPFENPTPFSNFFEMSLIFLIGAGLTVTLGRMTGSP
                     GHGWAVFACMSILFVAGFTVAYWAESHPNPTLHNVAQSSGPLQAGGNMEGKETRFGIA
                     DSALFATITTDASCGAINSWHDSFTPLGGMIPLLNIELGEIIFGGVGSGLYGMIIFVV
                     LSVFIAGLMVGRTPEYLGKKIEAYDVKMSMLYVLIFPLLILTLAAISVLYPAGLAGIT
                     NPGPHGLSQILYAYSSAAGNNGSAFAGLGVNTRWYNTTLGFTMLMGRFLMMIPMLALA
                     GNLARKKSIPPSPGTFPVNTALFTVLLTGVILIVGALTFFPALSLGPILEHLLLQHGT
                     LFS"
     gene            complement(4235..4324)
                     /gene="kdpF"
                     /locus_tag="VHE33_13085"
     CDS             complement(4235..4324)
                     /gene="kdpF"
                     /locus_tag="VHE33_13085"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HVT98434.1"
                     /translation="MNIITLTILAITVLLLGYLIAALLYPEKF"
     gene            complement(4512..5201)
                     /locus_tag="VHE33_13090"
     CDS             complement(4512..5201)
                     /locus_tag="VHE33_13090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT98435.1"
                     /translation="MAERILVVDDEIQITRVLRASLSAQRYDVRAANDPEEAVRLFED
                     WNPDLVITDLAMPGMSGVELCRAIRRQSAVPIIVLSVRDQEQTKVEALDAGADDYVTK
                     PFSIQELLARVRAHLRRAPERAGDGAVQVGDFLVDPAGHTIAVAGRPVHLTPKEFDLL
                     LLFARNAGKVLTHRVLLTAVWGAQSAQQPEYLRVFIGQLRKKLQGDTHKEYIQTEPWV
                     GYRFLPEGAAE"
     gene            5261..>6646
                     /locus_tag="VHE33_13095"
     CDS             5261..>6646
                     /locus_tag="VHE33_13095"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HVT98436.1"
                     /translation="MEKDTPRFIKILLRYVVLTAAALLIVAVFRRVFTANPATVALSF
                     LMLVLVTASRWRLAYSVYLSVLCTLLYNFFFLPPLGRLTIAEPNNWVALSAFLICSVL
                     VSHLSEQEHQHAENAERGRREVERLYEFSQQLLLQEDLRSVARATPSVAAAVFGFRAV
                     ALYVRDEDAVHSSDPHNELAPIVDLKLMATQGESPIMARNGCRVIPLALGMRSLGVMA
                     VMGGDYSPQIYEAIGSLVAIAVERAVAVERSSRLEASRQSERLRSALIDSVTHDLRTP
                     LTAIRAAATTMVSQLDLAEAERAELAAVVDEESARLDRLIGQAVEMAQLDAEAVQIKA
                     EPQDVRELMEMTVEEMRSLLKDRAVKVHVEPNLPRVPIDRSLVHRVLRHLIENAIRYS
                     PPESPIELGARRDEDRLQVTVADQGPGVELGEQPFIFDKFFRGSKQRGRAGGTGMGLA
                     IVKAIVEAHGGG"
BASE COUNT         1321 a         1870 c         2217 g         1238 t
ORIGIN      
        1 tccgggtcga gtccggaggc tgaggttgtg accaggccga tgggcaccgg ctgacccgga
       61 ttctgcttct gccagttggc gacgtcagtc tcgatgcgcg tctgcagcgc cttgttcgac
      121 tgcgccaggt tcgagccgcc ggaactggtc gcgtcccatc cattcccggc cgcggacggc
      181 cgcgagtgga agtagcgcgg gctggtgaag ccctggccga tgatcgccga gccgatgatc
      241 tcgccgttgc gagtgatcag ctgcccgttc gccttgtcgc gcatcgttag ctgcgcaatt
      301 cccgtgacta ccaggggata gatgatgcca agaagcaccg tcgttaccag cgtgtagcgg
      361 accgcgacga ggagactcga ccgcaccgta ggtcccgggt gcgacgatcc tgtcggtgtg
      421 atcagccgtt cttcgtgagc aaaagaggta gtcgctgcca taagagtctc ctcacccgtc
      481 aggccaggtg caggccgatg atagccaggt cgatgatctt gattccgatg aacggtacga
      541 taatgccgcc gagaccgtag agaacaaggt tgcgccgcaa cagcgcctgc gctgacatgg
      601 gttcgtactt cactcccttc agcgccagcg gaatgagcgc gataatgata atcgcgttga
      661 agatgaccgc ggagaggatt gcagaatgcg ggttgcccag gtgcatgatg ttcagcgcgt
      721 tgagcacggg aaagacgccg gcaaacatcg cggggatgat ggcgaagtac ttcgctacgt
      781 cgttagcgat ggagaacgtg gtcagcgctc ctcgcgtcat cagaagctgc ttgccgatct
      841 cgacaatctc gatgagcttg gttgggtttg aatcaaggtc aaccatgttg ccggcttcct
      901 tggcagcctg ggtgccggtg ttcatggcga caccgacgtc ggcctgcgcc agcgccggcg
      961 cgtcgttagt accgtccccg gtcattgcaa ccagcttgcc cctggcctgc tcgcgtttga
     1021 tcaggtccat cttgtctttg ggcttggctt cggccaggaa gtcgtcaacg cccgcttcgc
     1081 gcgcgatcac cgcggcggtg agcggattgt cgccggtgat catgatgctg cggatcccca
     1141 tggcgcgcag acgggcaagc cgatctttca gaccgccttt gacgatgtcc ttgaggtaga
     1201 caacgccgag ggcagtcgca ttctctgaga ccacaagcgg tgtgccaccc tggcgggcga
     1261 tttcgtcgac gcgatcgctg accggacggg gcaggctcga gccttgcgat tcgagccagc
     1321 gagcgatcgc atcggccgag cctttgcgaa tcttgctgcc aggcaggtcg acgccggaca
     1381 tgcgcgtctg cgcgctgaag gggacgaact cggcgcgcat gccgctgacg tcgcggccgc
     1441 gaagattgta cttgtccttg gcgagcacca cgatcgaacg cccttcgggc gtctcatccg
     1501 acaacgatgc gagctgggca acgtcggcca gccgctccgt tgtgatgccg ggggcaggga
     1561 agaactccgt cgcctggcga ttgcccaggg tgatggtgcc cgtcttgtcg agcaggaggg
     1621 tattcacatc gcccgcggcc tcgacagcac ggccggacat ggccagtacg ttgtactgca
     1681 ccagccgatc catccccgca atgccgatcg cagagagcaa agcgccaatc gtcgtcggga
     1741 tgaggcaaac cagaagcgag accagcacga agatggtctg cggcgcgtgg gagtagatgg
     1801 cgtagggctg cagcgtggcg acggcgagca gaaagatgac ggtgaggccg gccagcagga
     1861 tactgagcgc gatctcgttg ggagtcttct ggcgcgtggc gccttcgacc agcgcgatca
     1921 ttcgatcgag aaaagtctca ccggggttcg acgtgatccg gatcttgatc cagtcggaca
     1981 acacgcgcgt gccgcccgtt actgccgagc gatcgcctcc cgcttcccga atcaccggag
     2041 cggattcgcc ggtgatggcc gactcatcga ccgaggccac gccttcgatc acctcgccgt
     2101 cacccgcaat gaactgcccg gcctccacgc ggatgatgtc atcggcgcgc agctgggagc
     2161 tcgatacggc ttccgtcgat ccgtcgggac gaaagcgcca ggccatcgtc tcgcccttgg
     2221 ccttgcgcag cgtgtccgcc tgggctttgc cgcgcccttc cgcgatcgcc tcggcgaagt
     2281 tggcgaaaag gacggtgaac caaagccaca gcgtgatctg aagatcgaac cgaatgggga
     2341 gatggcggat cgattcttcg atgagcaatg ccgttgtgac gacgctgccc acttcgacca
     2401 cgaacatgac ggggttcttc atcatgacgc gcgggtcgag cttgcggaag gagtcgatga
     2461 ccgcctgacg gaggatttca gagttccaga gattttgttt tttcgccatt gaagtgatcc
     2521 gggttaggag aacaacgttc catgctgcag cagcaggtgc tccagaatgg ggccaaggct
     2581 cagcgccggg aagaaggtca gcgcgccgac gatgaggatc acgccggtaa gcagaaccgt
     2641 gaacagcgcc gtgttgacgg gaaacgtgcc aggcgatggg ggaatgctct tcttgcgggc
     2701 cagattgccc gccagagcca gcatgggaat catcatcagg aagcggccca tgagcatggt
     2761 gaaaccgagc gtggtgttgt accaccgcgt gttcaccccc agacccgcaa acgccgatcc
     2821 gttattgccc gccgccgaag aatacgcgta gaggatctgg ctcagcccat gcgggccagg
     2881 attcgtgatg cccgcgagcc cggcaggata aagcactgag atcgccgcaa gcgtgaggat
     2941 gagtaagggg aagatgagca cgtagagcat ggacatcttc acgtcgtagg cttcgatctt
     3001 cttgcccaga tactcgggcg tgcgccccac catgagtccg gcgatgaaca ccgaaagcac
     3061 aacgaagatg atcatgccgt acaggccgga gcctaccccg ccgaagataa tctcgcccaa
     3121 ctcgatattg agcagcggaa tcatgccccc gagcggtgtg aaggagtcgt gccaggagtt
     3181 aatggcgccg caactggcgt cggtggtgat cgtggcgaac agggccgagt ccgcgatgcc
     3241 gaagcgagtc tccttgccct ccatgttgcc gccggcctgc agcggaccgg aactttgcgc
     3301 gacgttgtgc agcgtgggat tcggatgcga ctcggcccag taggccacag tgaagccggc
     3361 cacaaacaga atcgacatgc aggcaaagac cgcccatccg tgaccgggag agccagtcat
     3421 gcggccgagt gtcacggtca ggccggcgcc aatcaggaag atcagcgaca tctcaaagaa
     3481 attgctgaaa ggggtgggat tctcgaaggg gtgagagctg ttggcgttga agaagccgcc
     3541 gccgttcgta ccgagttcct tgatagcttc ctgggaagcg accgggccct gggcgatggt
     3601 ctgcgtctgg tgctcgagag tatgggccac agtgtacggg tgcagattct ggatgacgcc
     3661 ctggctgaca agaaccagcg ccgcgacgat gcagacgggc agcagcacgt agaggcaggc
     3721 ccgtgttgta tcgacccaga agttgccgat ggttttcgat tcgcggcgag cgatgccgcg
     3781 caccagcgcg atggcgacgg cgatgccgac ggcggcgctg aagaagttgt gataggcaag
     3841 cgctgccatc tgggtgaagt aactcatgac cgcttcgccc gtatacgcct gccagttggt
     3901 gttcgtggtg aacgaggcgg ccgtattcca ggcgagaaat tgctcgaccc ccgggaagtg
     3961 ctgcgggttc catggcaggt gaagctggag gcgctcgatc aggtacgtca gcagcagcga
     4021 aacaaagctg aagagcaaca tagcggcggt gtactggccc caggtcatct cttcgccgcg
     4081 aataccggaa gcgcggtaga tcaggttctc gagcgggccc agaaacttgt atttgccgga
     4141 gaagacgccg ttcatgtaaa tgcccagtgg gcgcatacag accagcagta cgacaaaata
     4201 gaccagaaac tgcaaccatc cgttgaatgt catgtcagaa cttctccggg tagagcaggg
     4261 ctgcgatcag gtagccgagc agaagcacag tgatggcgag gattgtgagt gtgatgatgt
     4321 tcatgaatta cctcagcttc tcgcaggccg tgatatacag cagcgcgacg gcgaagaatg
     4381 caacggtgaa gacgatcatc agtaggtcag tcatagcgtt tcgagattta gtaaaaggcc
     4441 gccgcagcaa gggcgtgcta agggaggcat aaaaaattcg taagttcccc gaggaatgca
     4501 caggatggct gctattccgc cgctccctcg ggcagaaaac ggtagccaac ccagggctcg
     4561 gtctggatgt attccttatg ggtgtcgccc tggagtttct ttcgcagctg accgatgaac
     4621 acacgcaggt attcgggctg ctgtgccgat tgcgcgcccc agacagcggt gagcaaaaca
     4681 cggtgtgtga gcaccttgcc ggcgttgcgg gcgaagagga gaagcagatc gaactccttg
     4741 ggggtgaggt ggacggggcg gcctgcgacc gcgatggtgt gtccggccgg gtcgacaaga
     4801 aaatcgccta cctggacagc gccgtcgccg gcgcgctccg gggcgcgtcg caggtgcgcg
     4861 cgaacacggg ccaggagctc ctggatgctg aagggcttgg tgacgtaatc gtcggcgccg
     4921 gcgtcgagcg cttcgacctt cgtctgttcc tggtcgcgga ctgagaggac gatgatgggc
     4981 acggcggatt gccggcggat agcccggcac agttcaacgc cggacatgcc gggcatggcc
     5041 agatcggtga ttacgaggtc cggattccaa tcttcgaaga ggcgcacagc ctcctcggga
     5101 tcgttcgcgg cgcggacgtc atagcgctgg gcagaaagcg aggcgcgcag gacgcgcgtg
     5161 atctgaattt cgtcgtccac gacgaggatg cgttcggcca tgacaacttg ctggcgattc
     5221 atcgcagcgc cgcagcgtgg cggcggtatg atcgcctttc atggaaaagg atacgccgcg
     5281 attcataaag attctgttgc gatacgtcgt tttgacggcg gccgcgcttc tgatcgtggc
     5341 ggtattccgg cgcgtcttca cggcgaatcc agccaccgtg gccctgagct tcctgatgct
     5401 ggtgctggtt acggcgtcgc gatggcggct ggcgtattcg gtgtacctgt cggttctctg
     5461 cacactgctc tacaactttt tctttctgcc ccccctgggg cgcctgacga tcgcggagcc
     5521 gaacaactgg gtggcgctca gcgcatttct gatctgcagt gtgctggtga gccatttgtc
     5581 ggaacaggaa catcagcatg cggagaatgc cgagcgagga cggcgcgagg tggaacggct
     5641 ctatgaattt agtcagcagt tgctgttgca ggaggacctg cgcagcgtgg cacgcgccac
     5701 tccttcggtg gcagccgcag tctttggatt tcgcgcggtg gcgctgtacg tgcgggatga
     5761 agatgctgtg cattcctccg atccgcacaa tgaactggcg ccaatcgtgg atctgaagct
     5821 gatggccact cagggagaga gcccaatcat ggcgcgcaac ggctgtcgcg tgattcctct
     5881 tgcgctcggg atgcggtccc tgggcgtgat ggccgtgatg ggtggagact attcgccgca
     5941 gatctacgag gcgattggca gccttgtggc aatcgccgtg gaacgggcgg tcgctgtgga
     6001 gcggagcagc cggttggagg cctccaggca gagcgagcga ctgcgcagtg cgctgatcga
     6061 ctcagtaacc cacgatctgc gaacgcccct gaccgcgatt cgggcagcag caaccaccat
     6121 ggtgagccag ctggatttag ccgaagcaga gcgagcagaa ctcgcggcgg tcgtggacga
     6181 ggagagcgcc cggctcgacc ggctgatcgg acaggcggtg gagatggccc agctggatgc
     6241 ggaggcggta cagatcaagg cagagccgca ggatgttcgc gagctgatgg agatgacagt
     6301 cgaggaaatg aggtccctcc tgaaagatcg ggcagtgaag gtccatgttg agccgaatct
     6361 gccacgcgtg cccatcgatc gttcgctggt gcaccgtgtg ctgcggcatc tcatcgagaa
     6421 tgccatccgg tattcgccgc ctgaatcgcc gatcgaattg ggtgcccgca gggacgagga
     6481 tcgcctgcag gtgacggtgg ccgatcaggg gccaggagtt gagctcggcg aacaaccttt
     6541 catctttgac aagttttttc gcggcagcaa acagcgtggc cgggcgggcg ggaccgggat
     6601 gggactggcg atcgtgaagg cgatcgtgga ggcgcacggt ggcggc
//
LOCUS       DASZYN010000509         1819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_989161, whole genome shotgun sequence.
ACCESSION   DASZYN010000509 DASZYN010000000
VERSION     DASZYN010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989161"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VHE33_13100"
     CDS             complement(<1..671)
                     /locus_tag="VHE33_13100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HVT98437.1"
                     /translation="MSLALTTGCSHKKEVAYTPPPPPAAAAEPSASDSAASTVTPTSP
                     LPPAPSLSEDEQFVDTHRPIYSETGVASWYGPPYHNRVGANGQVFDENGISAAHRTLP
                     MGSLIRVVNLKTGQSAVMRVTDRGPFVGNRILDLSIGAAKAVGIRRAGLGQVRIDVFS
                     TPKPMDEGGRWCVQIGAFEHEKSALHLQSQLKREYQSANVIEFKGPTGYWVRIRPAND
                     DRKVAV"
     gene            complement(760..984)
                     /locus_tag="VHE33_13105"
     CDS             complement(760..984)
                     /locus_tag="VHE33_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98438.1"
                     /translation="MAETSNGRFENVGRKMDEHFGACGDRIEDDVKRVITYLNDKVVP
                     EVREHSSKALRIASEQLSRLADHLERTRRA"
     gene            1143..>1819
                     /locus_tag="VHE33_13110"
     CDS             1143..>1819
                     /locus_tag="VHE33_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98439.1"
                     /translation="MNLLHFIHPVCPVLFSALLLVLPAPLVRAQAVASLPDAPQPAAL
                     RAAAQAQEQNPPANPDPLAPPAHANPNSASPIQHASREKRKWAQFVDPGERIVPLYPH
                     DKWMYWLHMETTWYSLGPAFVSAGFGQLTGAPAYGSDSGAFGDRLGAAVIRQASMRFM
                     CGSFFPMVTKEDPRYYRKASGSYMGRAGWAAERAFVTQKDDGSHGFNYSDVLGHLAAS
                     ALTPLYYP"
BASE COUNT          325 a          666 c          479 g          349 t
ORIGIN      
        1 accgccacct tccgatcgtc gttcgcaggc cgaatcctca cccaataccc ggtcggcccc
       61 ttgaactcga tcacattggc gctctgatat tcccgcttca gttgcgactg aagatggagc
      121 gcggacttct catgctcgaa cgcgccaatc tgtacgcacc agcggccgcc ttcgtccatc
      181 ggcttcggcg tgctgaagac atcgatgcgc acctgtccaa gccccgcacg cctgatcccc
      241 accgctttcg ccgcaccaat cgacagatcc aggatgcgat tcccgacgaa gggccctcga
      301 tccgtcactc gcatcaccgc ggactgcccc gttttcaaat tcacgacccg gatcagcgac
      361 cccatcggca gcgtccgatg cgccgccgag attccgttct cgtcgaatac ctggccattt
      421 gccccgacgc ggttgtgata cggaggtccg taccagctgg caacgccggt ctcggagtag
      481 atcggccggt gcgtatccac gaattgctca tcttcgctca gcgagggagc cggcggaagc
      541 ggcgacgtcg gcgtcaccgt ggatgcggcg gaatcggacg cgctcggctc cgcagccgct
      601 gctggcggtg gcggcggcgt gtacgcaacc tctttcttat gcgagcaacc ggtcgtcagc
      661 gcaaggctca cgcccagcaa aacggcccct cgccaaccag gccgcacagt cagattgttt
      721 cgatccaggg ggagtcgatt ggccatctca aaagtcgttt caggctcgtc gcgtgcgctc
      781 aagatgatcg gcgagccgcg acagctgctc cgaggcaatc cgcaaagcct tcgaggaatg
      841 ctcccgcact tccggcacca ccttgtcatt cagatacgtg atcacgcgct tcacatcgtc
      901 ttcaatgcgg tcgccgcagg cgccaaaatg ttcgtccatc ttgcgcccaa cattctcaaa
      961 acgaccgttg ctggtttccg ccatgggatg ctccctgcca gactactgct gcctcttagt
     1021 attgtcggcg gcgacacgcc ggtcaatcca ctttgaatgc ccccgcgcgt gtgccaaatc
     1081 agatacctgc gtggccaatg catcccgaat ccagaaactc tcctccaatc cccagggtac
     1141 ccgtgaacct tttgcacttc atccatccag tctgtcctgt tctgttttct gctctgctcc
     1201 tggttctccc agcgcccctt gtccgggcgc aggccgtcgc gtcccttccg gacgcgccgc
     1261 aaccggccgc tctccgggcc gctgcccagg cccaggaaca gaacccgcca gcaaatcccg
     1321 accccctggc gcctccagca cacgccaacc ccaattcggc cagccccatc cagcatgcct
     1381 ctcgtgaaaa gcgcaaatgg gcccagttcg tcgatcccgg cgaacgcatc gtgcccctct
     1441 accctcacga caagtggatg tactggctcc acatggaaac cacctggtat tcgctgggcc
     1501 cggccttcgt ctccgctggc ttcggccagc ttaccggcgc gcccgcctac ggcagcgaca
     1561 gcggcgcctt cggcgatcgc ctcggtgcgg cggtcatccg ccaggccagc atgcgcttta
     1621 tgtgcggcag cttctttccg atggtgacca aagaagaccc ccgctactac cgcaaagcga
     1681 gcggcagcta tatgggccgc gccggctggg ccgccgaacg cgccttcgtg acccagaaag
     1741 atgacggcag tcacggcttc aactactccg acgtcctcgg ccacctcgct gcatcggcgc
     1801 tcaccccgct ctactatcc
//
LOCUS       DASZYN010000510         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1049528, whole genome shotgun sequence.
ACCESSION   DASZYN010000510 DASZYN010000000
VERSION     DASZYN010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049528"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VHE33_13115"
     rRNA            complement(<1..832)
                     /locus_tag="VHE33_13115"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(1303..1378)
                     /locus_tag="VHE33_13120"
     tRNA            complement(1303..1378)
                     /locus_tag="VHE33_13120"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1343..1345),aa:Ala,seq:tgc)
     gene            complement(1461..1537)
                     /locus_tag="VHE33_13125"
     tRNA            complement(1461..1537)
                     /locus_tag="VHE33_13125"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1501..1503),aa:Ile,seq:gat)
     gene            complement(1815..>2050)
                     /locus_tag="VHE33_13130"
     rRNA            complement(1815..>2050)
                     /locus_tag="VHE33_13130"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          408 a          651 c          534 g          457 t
ORIGIN      
        1 cgaggctttt tcaacagaca ccggttcggc cctccagtgg gtgttacccc accttcagcc
       61 tggtcatggc tagatcacta ggtttcgggt ctaatccgac gaactgaacg ccctgttcag
      121 actcgctttc gctgcgccta cgcctatcgg cttaagcttg ctcgtcaaat taagtcgctg
      181 gcccattata caaaaggtac gacgtcaccc aggacgaacc ttgggctccg tctgtttgta
      241 ggcattcggt ttcaggtctc tttcactccc ctcgtcgggg tgcttttcac ctttccctca
      301 cggtactggt tcgctatcgg tcgctgagga gtacttaggc ttggagggtg gtccccccat
      361 gttcagacag gattgcacgt gtcccgcctt actcaaggat aatccttcgc attacccgta
      421 cggggctgtc acccgctaag gcacgtcgtt ccaaacgttt ccggttgtct cgggattatc
      481 actggcctgg tccgcgttcg ctcgccacta ctagcggagt ctcggttgat gtcctttcct
      541 ccaggtactg agatgtttca gttccctggg tttgctttga accccctatt ttattcagga
      601 gtccaatacc ttcttttgat accggaagcc ggacgacagc agcgccggcg gcggatcgct
      661 ccgtcgccca cattctgtcg tctgatttcc ggtatcgaag gtgggtttcc ccattcggaa
      721 atccgcggat caaagcttct tcgcagctcc ccacggctta tcgcagcgta gtacgtcctt
      781 catcgcctct cagcgccaag gcatccacca aatgccctta agacacttga ttgctctcat
      841 tatcaatgcc catcgcactc ggcgtgcgta ccggcgcaac cttgcggtcg ctccggcggg
      901 acactgatta gaaagaccag cttgcgagat cgatccgacg gcaggcggtc aggcccagcc
      961 gacatttaag cgtgaccgta caccgcttgg tcggcaagtt tccttgccgg caagccgacg
     1021 cacgaccgct cggatcaatc ccctcttcac gatgtcagac aacccgcatc gcgccttcgg
     1081 cgaagccgaa caacgcatgc gaattcaaaa atcacggaca agcttcattt gcgcttcgac
     1141 gtttatgagc ttcgacgctt ggcgtcacag ctacgcccct agagcttcga cgcttagcgc
     1201 caagcccgcg ctcctagaac cacggcgcga ccgcatcggc gcttatccca tcggcccgcc
     1261 gtgacgaaac gtcgtcggcg ccaagtctgt cggcgcgaag tctggtggag ccagacggga
     1321 tcgaaccgac gacctcctgc ttgcaaagca ggcgctctcc caactgagct atggcccctt
     1381 caggtgtcgg gtttcaggtg tcaggcgcca gcgcaccaca ccggaaagct gacacttggc
     1441 acccgatacc tgacacctgg tggtgggcct gggaagattt gaacttccga cctcacgctt
     1501 atcaagcgcg cgctctaacc agctgagcta caagcccata ccagacgtca ggtgtcaggt
     1561 atcgggtgtc agcgcagcat caagctgaca cctgatacct gacacttggc acctgaacat
     1621 ggcacctgaa ccgagctcgt ccgcgaagaa agagaaacgt agacggcggc gtcccgccag
     1681 caggtgtcgg gcatcagata tcaggtgtcg aaacaccgaa ccgatttccg cacccatgtt
     1741 gttctaagct caagtgtagg ccgagatcgc caagctgacg cctggcaccc gatacctgac
     1801 acctgaatcc ttagaaagga ggtgatccag ccgcaggttc ccctacggct accttgttac
     1861 gacttcaccc cagtcgctga ccctaccgtg gccggctgcc tcccttgcgg gttagcgcac
     1921 cgtcttcagg taaagccaac tcccatggtg tgacgggcgg tgtgtacaag gcccgggaac
     1981 gtattcaccg cagcgtgctg atctgcgatt actagcgatt ccagcttcat ggggtcgagt
     2041 tgcagacccc
//
LOCUS       DASZYN010000511         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_217229, whole genome shotgun sequence.
ACCESSION   DASZYN010000511 DASZYN010000000
VERSION     DASZYN010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217229"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VHE33_13135"
     CDS             <1..385
                     /locus_tag="VHE33_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98440.1"
                     /translation="LYHASRKQREAILHQFLRACMDGDMEGLLGLLANDIVVYADSGG
                     KAPAARNPVSGADRVARYLLGLVRKALEGSRFHVALVNGQPGIIHYVNGVPSAVLTLD
                     MLDGQIQEIDVITNPDKLQGIAALN"
     gene            complement(469..1344)
                     /locus_tag="VHE33_13140"
     CDS             complement(469..1344)
                     /locus_tag="VHE33_13140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVT98441.1"
                     /translation="MDEHEWLAEQFEANRTHLQAVAYRMLGSLSEADDAVQESWLRLS
                     RSDTSGIENLVGWLTTVVARICLDMLRSRNSRREESLEATVPESIESREKGSDPEQEA
                     LLADSVGLALLVVLDTLPPAERLAFVLHDIFAMPFEEIAPIVERSETATRQLASRARR
                     HVRGAEMARPADLTRQRAVVDAFLAAARAGNFEALLTMLDPEVVFRHDGAVLARSVAR
                     EVRGAPAVARQFSGRAQGARTALVDGVVAAIVAPLGHLFLVFYPTIRNGKIVEIEAVT
                     DPARLSKLRLAVLDD"
BASE COUNT          312 a          523 c          503 g          354 t
ORIGIN      
        1 tctgtatcat gcgtccagga agcagcggga agccatcctg caccagttcc tgcgcgcctg
       61 catggatggt gatatggagg gtctcctggg tctgctggcc aacgatatcg tcgtctatgc
      121 tgatagcggt ggcaaggctc ctgcggctcg caatcccgtc tctggcgcgg acagggtggc
      181 gcgctatctc ctcggtcttg tgcgcaaagc cctggagggt tcccgtttcc acgtagccct
      241 cgtcaacggt cagcccggca taattcatta cgtgaatggc gtgccttccg ctgttctgac
      301 cctggatatg ctcgatgggc agatccagga gattgatgtg atcactaatc ctgacaaatt
      361 gcaaggaatc gcggccctta attaaggccg ctacgtaccc tgcccggttc catcgcaggt
      421 gtcccgaacc gccgggcggg tacgtagcgt ttaacgggtg gaggtttgtc agtcgtcgag
      481 gactgccagc cgcaacttgc tcaggcgtgc tggatcggtg actgcctcga tctcaacaat
      541 cttgccgttt ctgatggtgg gatagaaaac gagaaacaga tgtccaagcg gcgccacgat
      601 ggctgccaca actccatcca ccagggccgt gcgggcgccc tgagcgcgtc ctgagaactg
      661 gcgggccacg gctggcgcgc cacgaacctc tctggccaca cttctggcca ggacagcgcc
      721 gtcatgccgg aacacaacct ccgggtcgag catcgtgagc agcgcctcaa agttcccggc
      781 gcgcgcggcg gccaggaagg catccaccac tgctcgctgg cgcgtgagat cggcaggtct
      841 ggccatttcc gcgccccgca catggcggcg tgcgcggctg gcgagctgtc tggtcgccgt
      901 ttccgaacgt tccacgatag gcgcgatctc ctcgaaaggc atagcgaaga tatcgtgcag
      961 cacgaaggca agccgctcgg cgggtgggag cgtgtcgaga acgaccagca gagccagccc
     1021 caccgagtcc gccagcagcg cctcctgctc cgggtcgctg cccttctcgc ggctctcgat
     1081 ggactccggc accgttgcct ccagtgattc ctcgcgccgt gagtttcgtg agcgtaacat
     1141 atcgaggcaa atgcgcgcca ccaccgtggt cagccatcct accaggtttt caatgccgct
     1201 cgtatcggag cggctgaggc gcagccacga ctcctgtacg gcgtcgtcag cttcactcag
     1261 cgagccgagc atgcggtagg ctacagcttg cagatgggtc cggttggcct cgaattgttc
     1321 tgccagccat tcgtgttcgt ccatcaattc atcccttcgc tttattcgat cagaggcgct
     1381 atgggggtct gatctcatca atgtgacgcg tgtactcatc cgccggtcac atttgttcat
     1441 ttcatttcct cataggactg acgagcaaaa cccggttgat gtgacagaca ggggcgggtg
     1501 gacatatgac cattcaccag atacggcctg atcccggcag gcaggcgcgt gcccttttcc
     1561 tccagcaact catcggcaat gagacgcggg cagtgagtgc gcagagccag ggcaatcatt
     1621 aagcctttgc gggcagtgag ttcaaacgct cctttttcct ggctctaccg ggccttcagc
     1681 caaccgcatg tt
//
LOCUS       DASZYN010000512         6659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_343172, whole genome shotgun sequence.
ACCESSION   DASZYN010000512 DASZYN010000000
VERSION     DASZYN010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343172"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..730)
                     /locus_tag="VHE33_13145"
     CDS             complement(98..730)
                     /locus_tag="VHE33_13145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT98442.1"
                     /translation="MTTWDILTTSIPHRHTRLLALLETLDAQMDASVRMILYRDNLEV
                     PYGDKTRALIEASDADYVSSIDDDDSVAPDFIPLVLEALKSEPDYVGFRVRWTRDGAR
                     QWPVEHNLEFGCWEDRPQLLCRDIAQFNPIRRELALPEAWAGAWRADRDWSDAVRATG
                     LVKTQVFIPGEMYYYQNTSYDHFQMARSPVQGPMPELPSYPWLRQIGPYA"
     gene            complement(727..1086)
                     /locus_tag="VHE33_13150"
     CDS             complement(727..1086)
                     /locus_tag="VHE33_13150"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVT98443.1"
                     /translation="MTDRFEDERGVIQDLLGAPIDGITEIFTREGAVRGNHVHMQTTQ
                     WTYVVSGRMLAVAKLIDGSLAERELWPGQMLEETAAVPHAWKALQDTKVLVFTRGPRS
                     GAGYESDTVRLKEPILR"
     gene            complement(1079..2215)
                     /locus_tag="VHE33_13155"
     CDS             complement(1079..2215)
                     /locus_tag="VHE33_13155"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT98444.1"
                     /translation="MSGKETTECQGCGGQELHPLFDMGVQPLAENSNGERYPLVLIQC
                     LDCFLVQLSYQADPAVMFQADHPYSTGNSEALRHHYTMLAHNMRMDLPEGSLIVDIGA
                     NDGTFLANFTRWQDLTLVAVEPTDQILKAPDQVIKFQAPFTSELARRIAGDGAIGRAR
                     YVTAMNVLAHVPDVHDFLDGVTQLLAPGGQFVTENHDFYSITDGLQIDTIYHEHLRYY
                     TVASLSRLLEQHWLRVTRVEQTRMHGGSFRIWARDTRHDWTSDAAEAAMDLTELLGEI
                     TGKGHAVYGIGAATRATPLIHFAGLAPYIAYVCEVAGSAKIGTFIPGTDIPVVDEADL
                     VRHQPAYALLFAWHMKDYIVPKLRAAGYRGKFIVPLPKPEVIGD"
     gene            complement(2205..3179)
                     /locus_tag="VHE33_13160"
     CDS             complement(2205..3179)
                     /locus_tag="VHE33_13160"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HVT98445.1"
                     /translation="MGVQGLMRYLVTGGAGFVGSALVKKLAREGHEVVVLDNMSRGKP
                     YRLSGVNCEVIAGDVNITSDVTGAMHGCDGVAHLAYLQGTQTFYAEPRQVLETAVRGM
                     LNVLHACEVTGCADLLLVSSSEAYQVATVVPTPEDIPLTVPDVLNPRYSYGGGKIACE
                     LMALAWQRTGVLDRVVIARPHNIYGPDMGWEHVIPEFCERMNRLTREQPEGIIDFPIQ
                     GTGEETRSFCYIDDCTDQLARLTHPAAPGGIYHVGTMDERTIADVAHSVAACYNREIK
                     VVPGTLPKGSPPRRLPDTAKITALAGLPKVDFPHGIGPTVHWYRVNER"
     gene            complement(3161..4228)
                     /locus_tag="VHE33_13165"
     CDS             complement(3161..4228)
                     /locus_tag="VHE33_13165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVT98446.1"
                     /translation="MKILASDDGGTGCAWYRMYVPLQAVDKFSPDVSVTFRRGGHPVS
                     ENPHPMVKVADAEEHDVVIAQRSSSYEGLGLWRRWGSSITRRTVYENDDDVFSITPEN
                     FAAWQTYKEGTTIREATLRYCRTASLITTTTPHLGDRFREMCPRVPVEVLPNYIPEWV
                     LSLPRDTEDRPKRLGWMGGASHARDIHTATPAVRRFMKRFPDWELHINGVDYRSSFKC
                     PPERSFHTPWIHVVDHPELYYRSIDFDIGICPLLSTTFSRSKSAVKALEYFSRGIPVV
                     ASDVEPYTRFIDHGVDGFIAKTDHEWLTYLTALAKDDVLRKQMGENAKEKARLNTIEL
                     HYNEWVNAYKMLFPVGWEFKG"
     gene            complement(4225..4380)
                     /locus_tag="VHE33_13170"
     CDS             complement(4225..4380)
                     /locus_tag="VHE33_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98447.1"
                     /translation="MIVVDNLEDRAKAAYGRYCDAAARATGLAQSGTWERLTPAVREY
                     WIAAVTE"
     gene            complement(4448..4786)
                     /locus_tag="VHE33_13175"
     CDS             complement(4448..4786)
                     /locus_tag="VHE33_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98448.1"
                     /translation="MNLHHRRAAAVLVVTAVLDVALGVLYGLAERIHILHGMYCAVAT
                     ATTVGCDIPPDNGFSYVLSAVMMLTVVPLFAAVFSFFTSGLTADHVDTRHEQIKEHLS
                     GTQRLPEAGE"
     gene            complement(4783..5157)
                     /locus_tag="VHE33_13180"
     CDS             complement(4783..5157)
                     /locus_tag="VHE33_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98449.1"
                     /translation="MKLFAALTATLAGIFLYSGIAHPFGLRFALGIWPVPQGTPWTYQ
                     LESGFVPALTVLTLLSLVSGAWKHVNCHHRWCWRTGKHKVNGTPWCSIHESEARPERT
                     ENEILQSIEATLASLAEVLRDR"
     gene            complement(5250..5543)
                     /locus_tag="VHE33_13185"
     CDS             complement(5250..5543)
                     /locus_tag="VHE33_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98450.1"
                     /translation="MADSLSWPDSWGPIHKATTDGKTAGREEGQVTQHHVTGGDAVRH
                     PVNYNATDARHGHENNIGGNWPSIDHMSGPVTEHDWDHSTGHFPDGPGPWRQT"
     gene            complement(5543..5785)
                     /locus_tag="VHE33_13190"
     CDS             complement(5543..5785)
                     /locus_tag="VHE33_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98451.1"
                     /translation="MQDLSSLVKGLMGDSSMHAGNTVSVNPPFSPEGSESEATQGIPT
                     HSRPELDVPPVSNTKDRTVKASSSFSMSETSWKKLK"
     gene            complement(5924..6454)
                     /locus_tag="VHE33_13195"
     CDS             complement(5924..6454)
                     /locus_tag="VHE33_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98452.1"
                     /translation="MAGVPDGVNAGRIGRPARQSPARKYNGRNEPGVDPTNELGQTPS
                     EIVGGFSQNYSTGAPGTRGGSAPANLPTIQAGQLDPGLAGVTGSQLTNTGMPGSDGAR
                     NGVRGETVTYTDPFGFLGGVNRSVSVQGQVDGEGDWTQANDAGYSGGPQLPILQNNRP
                     TSTGMGQGRVRTHHRD"
BASE COUNT         1040 a         2238 c         2193 g         1188 t
ORIGIN      
        1 aaccaggtgt caccctcgca ggtcgcgtcg gcggcgtcac tcagccgtgc gacgtttccc
       61 ggccgtcccc ggctggggat gaccatcagc aggtctctca tgcatacggt cctatctggc
      121 gcagccacgg gtacgacggc agctccggca tcggcccctg caccgggctg cgggccatct
      181 ggaagtggtc gtagctggtg ttctggtagt agtacatctc gcccgggatg aacacctggg
      241 ttttcaccaa gccggtggcc cgcactgcgt ccgaccagtc ccggtctgcc cgccaggctc
      301 ccgcccaggc ttccggcagg gccagctcgc gccggatggg gttgaactgg gcgatgtccc
      361 ggcacagcag ctgcggccgg tcctcccagc agccgaattc caggttgtgc tcgaccggcc
      421 actggcgggc gccgtcccgc gtccaccgca cccggaaccc gacgtagtcc ggctcacttt
      481 tcagcgcctc cagcaccagc gggatgaagt ccggggcgac tgagtcgtca tcgtcaatgc
      541 tggagacgta gtccgcgtcc gacgcctcaa tcagtgcccg ggtcttgtcg ccgtagggca
      601 cctcaaggtt gtcccggtac aggatcatcc gcacgctggc gtccatctgc gcgtccagag
      661 tctccagcag cgccagcagc cgggtgtgcc ggtgcgggat cgacgtggtg aggatgtccc
      721 aggtggtcac cggaggatcg gttccttcag ccggacggtg tcgctctcat aacccgctcc
      781 cgagcggggg ccccgggtga agaccagcac cttcgtgtcc tgcagtgcct tccaggcatg
      841 gggaacagcc gccgtctcct ccagcatctg gcccggccac agctcccgct ccgccaggct
      901 gccgtcgatc agcttggcga ccgccagcat gcgcccggag actacgtacg tccactgcgt
      961 ggtctgcatg tgcacgtggt tgccccggac ggcgccctcg cgggtgaaga tctcagtgat
     1021 gccgtcaatt ggcgccccca gcaggtcctg gatcaccccg cgctcgtcct caaatctgtc
     1081 agtcaccgat gacctccggc ttcggcagcg gcacgatgaa cttcccccgg tagccggccg
     1141 cccgcagctt ggggacgatg tagtccttca tgtgccaggc gaacagcagc gcataggcgg
     1201 gctggtgcct gaccaggtcc gcctcgtcca cgaccgggat atcagtgccc ggtatgaatg
     1261 tgccgatctt ggcgctgccg gctacctcgc acacgtacgc gatgtacggc gcgagcccgg
     1321 cgaagtggat cagcggagtc gcccgggtgg cagcgccgat cccgtagacc gcgtggccct
     1381 tcccggtgat ctcgcccagc agctcagtca ggtccatcgc ggcctcggcg gcgtctgacg
     1441 tccagtcgtg gcgggtgtcc cgggcccaga tccggaacga gccgccgtgc atccgggtct
     1501 gctccacccg ggtcacccgc agccagtgct gctccagcag ccgcgacagc gacgcaaccg
     1561 tgtagtaccg caggtgctcg tggtagatgg tgtcgatctg cagcccatcg gtgatgctgt
     1621 aaaagtcatg gttctcggtg acgaactgcc cgcccggggc cagcagctgg gtcacgccgt
     1681 cgaggaagtc gtgcacgtcc ggcacgtgag ccagcacatt catcgcggtg acgtaccggg
     1741 ccctgccgat tgccccgtct cccgctatgc ggcgggcaag ctcactggtg aacggagcct
     1801 ggaacttgat gacctggtcc ggggccttca ggatctggtc tgtcggctca acagccacga
     1861 gtgtcaggtc ctgccaccgg gtgaagttgg ccaggaacgt gccgtcgttg gccccgatgt
     1921 caacgatcag cgagccctcc ggcaggtcca tccgcatgtt gtgcgccagc atcgtgtagt
     1981 ggtgccgcag ggcttcggag ttgcctgtcg agtacgggtg gtccgcctgg aacatcacgg
     2041 ccgggtcagc ctggtaactg agctgcacca ggaagcagtc caggcactgg atcagcacca
     2101 gggggtagcg ctcaccgttg ctgttctctg ccagcggctg cacgcccatg tcgaacagcg
     2161 ggtgcagctc ctggcccccg cagccctggc actcggtcgt ctctttaccg ctcattgact
     2221 cggtaccagt gcacagttgg gccgatgcca tgcgggaagt ccaccttggg caggccggcc
     2281 agggcagtga tcttggcggt gtccggcagg cggcggggcg gggagccctt gggcagggtg
     2341 ccgggaacga ccttgatctc ccggttgtag caggcggcga ccgagtgcgc gacgtcagcg
     2401 atggtgcgct cgtccatcgt gcccacgtgg taaatcccac cgggagcagc cgggtgtgtc
     2461 agccgggcaa gctggtccgt gcagtcatcg atgtagcaga acgaccgcgt ctcctcgccc
     2521 gtgccctgaa tggggaagtc gatgatgcct tccggctgct cccgggtcag ccggttcatc
     2581 cgctcgcaga actccgggat gacgtgctcc cagcccatgt ccgggccgta gatgttgtgc
     2641 gggcgggcga tgaccacccg gtccagtacc ccggtgcgct gccaggccag ggccatcagc
     2701 tcgcaggcga tcttgccgcc gccgtaggag tagcgcgggt tgagcacgtc cgggacagtc
     2761 agcgggatgt cctccggcgt gggcaccacc gtggcaacct ggtacgcctc actggacgac
     2821 accagcagca ggtccgcgca cccggtgacc tcgcaggcgt gcagcacatt gagcatgccc
     2881 cgcacggccg tctccagcac ctgccggggc tcggcgtaga acgtctgggt gccctggagg
     2941 taggcaaggt gggcgacccc gtcgcagccg tgcatcgcgc cggttacgtc ggaggtgatg
     3001 ttcacgtccc cggcgatgac ctcgcagttc accccggaca ggcggtacgg cttgccccgg
     3061 gacatgttgt ccagcaccac aacctcgtgg ccctcgcggg caagcttctt caccagggct
     3121 gagccgacga acccggcgcc ccccgttacc aggtaccgca tcagcccttg aactcccatc
     3181 cgaccgggaa cagcatcttg taagcgttaa cccactcatt atagtgaagc tcaatagtgt
     3241 tcaggcgtgc cttttccttc gcgttctcac ccatttgctt acgcaggacg tcatctttcg
     3301 caagagccgt caggtacgtc agccattcat ggtctgtctt cgcaatgaag ccgtccacac
     3361 catggtcgat gaaccgggtg tacggctcaa cgtcgctggc cacgacgggg atgccccggc
     3421 tgaagtactc cagcgccttg accgcgctct tggaccggct gaaggtggtc gacagcagcg
     3481 ggcagatgcc gatgtcgaag tcaatcgagc ggtagtacag ctccgggtgg tccacgacgt
     3541 gtatccacgg ggtgtggaag gaccgttccg gcgggcactt gaacgaggag cggtagtcca
     3601 ccccgttgat gtgcagctcc cagtccggga accgcttcat gaaccgccgc acagccgggg
     3661 ttgccgtgtg gatgtcccgg gcgtgagagg cgccgcccat ccagccgagc cgcttcggcc
     3721 ggtcttcggt gtcccggggc aggctcagca cccactccgg gatgtagttc ggcagcacct
     3781 cgaccggaac gcgggggcac atctcgcgga agcggtcgcc caggtgcggg gtggtcgtgg
     3841 tgatcaggct ggccgtgcgg cagtagcgca gcgtcgcctc gcggatcgtg gtgccctctt
     3901 tgtaggtctg ccaggccgcg aagttctccg gcgtgatgct gaaaacgtcg tcgtcgttct
     3961 cgtagaccgt gcggcgggtg atgctgctgc cccagcggcg ccacaggccc agtccctcat
     4021 acgagctgga gcgctgggcg atgacaacgt catgctcctc ggcgtccgcg accttgacca
     4081 tcgggtgagg attttccgaa accgggtgac cgccgcgccg gaacgtcacg ctgacatcag
     4141 gggaaaactt gtccaccgcc tgcagcggaa cgtacatccg gtaccaggcg cagcctgtgc
     4201 ccccgtcgtc cgaggccagg atcttcactc ggtgaccgcc gcaatccagt actcgcggac
     4261 ggcaggtgtc agccgttccc aggtcccgga ctgcgcgaga cccgtcgccc gcgccgccgc
     4321 atcgcagtag cggccgtacg ccgccttcgc ccggtcctcc aggttgtcaa ccacgatcat
     4381 gcccagctcg tgccagaact ggagagcctc gtcccgggaa agctcaaggg tgactcttac
     4441 ccggtcgtta ctccccggct tccgggaggc gctgagtgcc actgagatgt tcctttatct
     4501 gttcgtgcct cgtgtctacg tggtcagccg tcaggccact ggtgaaaaaa gagaacacag
     4561 cagcgaacaa aggcaccacg gtgagcatca taacggcgga cagcacatag gagaagccgt
     4621 tgtcaggcgg gatgtcacac ccgacggttg tcgccgtcgc gacagcgcag tacatcccgt
     4681 gcagaatgtg aatgcgctcc gccaggccgt acaggacacc gagcgccacg tcgaggaccg
     4741 ccgtcaccac gaggacggcg gcagcacggc ggtgatgcag gttcatctgt cccgcagcac
     4801 ctcggccagt gaggcgagag tggcctcgat ggactgcagg atctcgttct ccgtgcgctc
     4861 cgggcgcgcc tcagactcgt ggatactgca ccagggcgtg ccgttcacct tgtgcttccc
     4921 ggtgcgccag caccagcggt gatgacagtt gacatgcttc caggcaccgc tgaccagtga
     4981 cagcagcgtc agcactgtca gggcggggac aaacccggac tccagctgat aggtccaggg
     5041 cgtcccctgc ggcaccggcc agatccccag ggcgaaccgc agcccgaacg gatgggcgat
     5101 cccggagtag aggaagatgc ctgcgagcgt cgcagtgagc gcagcaaaca gcttcaccct
     5161 gtctcccgca aggtaagccg gctgtcccgc cgtgctccca aggggtccgc ctggagcacg
     5221 gcgggacagt tagcgttagt ttacattaat cacgtctggc gccacggtcc cgggccgtcc
     5281 gggaagtggc cggtcgagtg gtcccagtcg tgctcggtaa ccgggccgct catgtggtcg
     5341 atgctcggcc agttgccgcc gatgttgttc tcgtggccgt gccgggcgtc ggtcgcgttg
     5401 tagttgaccg ggtgccggac cgcgtcgccg ccggtgacgt ggtgctgggt cacctggccc
     5461 tcttcgcggc cagcggtctt gccgtcagtc gtcgccttgt ggatcgggcc ccaggagtcg
     5521 ggccaggaca gtgagtcggc cattacttca gcttcttcca gctggtctcg gacatggaga
     5581 acgacgagga cgccttgacg gtcctgtcct tggtgttgct gaccgggggg acgtcaagct
     5641 ccggccggga atgggtcgga atcccctggg tcgcctcgga ctcactgcct tccggcgaga
     5701 acggcgggtt cacgctgacg gtgttgcccg cgtgcatgga agagtcaccc atcagaccct
     5761 tcaccaggct gctcaggtcc tgcaccggcc acctcctctt gctgtgctga cggggtgagg
     5821 ctgaagccag cgggggcctg cggcagctcg tcactgtgcc cggccccgaa cgccccggcc
     5881 agcggcacgt ccggccacct gacccccgac tggtccttca cggtcagtcc ctgtggtgag
     5941 tgcggacccg gccctggccc atgccggtcg aggtgggccg gttgttctgc agaatcggca
     6001 gctgcgggcc gccggagtag ccggcgtcgt tggcctgggt ccagtcgccc tcgccgtcaa
     6061 cctggccctg gaccgacacc gagcggttca cgccgcccag gaagccgaac gggtcggtgt
     6121 aggtgacggt ctcgccccgg acaccgttgc gggcgccgtc gctgccgggc atgccggtgt
     6181 tcgtcagctg ggagccggta acccccgcca gacccgggtc gagctggccc gcctggatcg
     6241 tcggaaggtt cgcaggggcc gagccgcccc tggtgccggg agccccggtg ctgtagttct
     6301 ggctgaaccc gccgacaatc tcgctagggg tctggccaag ctcgttggtc gggtcgacac
     6361 ccggctcatt gcggccgttg tacttccggg ccggggactg ccgcgccgga cggccgatcc
     6421 gccccgcgtt aacgccgtca ggcacgcctg ccatagtgaa ctcctctggt tgccgctaac
     6481 accagtgtag agacctagag ccgctctcca tagcgcccca tcagctcgtt cttggtcatt
     6541 cccgcagctt cctgagggtc agcgccgttg tgcaccgccc aggtgatcca gtcggccttg
     6601 gattcgttgg cgtacgggcg ccgcaccggg gcttccgccg tctccggcac ctgctggcc
//
LOCUS       DASZYN010000513         2007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_601266, whole genome shotgun sequence.
ACCESSION   DASZYN010000513 DASZYN010000000
VERSION     DASZYN010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601266"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..696)
                     /locus_tag="VHE33_13200"
     CDS             complement(145..696)
                     /locus_tag="VHE33_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98453.1"
                     /translation="MDGIRIFRWALGLLVLTGAALASGQELTPDAAKAMLDQQGAATN
                     ASDFTLSQEQAHALTALSPDLLASLQIETNQPCLPNPGDKRVLRHQFAACQGIFPAGI
                     SDQNPGVYLKVGRAFHWHIVEISGIVDPTEDHTQRLVGYTWEYDFSGLPAEVQKVLSQ
                     GSRHEGHSLFQLDGGTWKWLRYQ"
     gene            complement(896..>2007)
                     /locus_tag="VHE33_13205"
     CDS             complement(896..>2007)
                     /locus_tag="VHE33_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HVT98454.1"
                     /translation="RDFAAREIAPNVLRWDEASEFPMEVVKQLGRMGLLGVIFPTEYG
                     GAGLGYVDYMLAIEELSAVDGSIGIIVAAHNSLCTNHIFLAGSEAQKKKYLPKLTTGE
                     HLGAWGLTEPGSGSDASAARTTAVRKGDHYVLNGTKTFITNGHYADVSVIIAVTNKEE
                     GTHGLSAFIVEKGTPGFRAGKKENKLGLRASDTSELIFEDCAIPAENLLGQEGEGFVD
                     AMRTLDGGRISIAALSLGIGKGALDASVKYVKQRKQFGKAIAEFQGIQWKLADMATEL
                     DAARLLTLRAAVLKDAGKRVTQESAMAKLYASEVAVRICNEAVQLHGGYGFIKDYPVE
                     KFYRDVKLCTIGEGTSEIQRMVIGREILKVHPSRG"
BASE COUNT          389 a          730 c          505 g          383 t
ORIGIN      
        1 ggttcgagga tggcccgctg ctcggcaacc ttctcctcaa acgacaggcc gaccatccag
       61 gagtaatgac gtgccagcag attgcgatag tggtcttcaa ccgtcggcat ggcgtcattg
      121 tagacgcgcc gtcccccacc cgccttactg gtaccgcagc cacttccatg tcccgccgtc
      181 tagctggaac agcgagtgcc cctcatgccg cgatccctgg ctcagcacct tctgcacttc
      241 ggccggaagc cccgagaagt catactccca cgtgtagccc accagccgct gcgtgtggtc
      301 ctccgtcgga tcgacaatcc cgctgatctc cacgatgtgc cagtggaagg cgcggcccac
      361 cttcaggtac acgccgggat tctgatcgga gattcccgcc ggaaaaattc cctggcacgc
      421 cgcaaactgg tgacgcagca cccgcttgtc cccaggattc gggagacagg gctggttcgt
      481 ctcaatctga agagaggcga gcaggtcggg actgagcgct gtgagcgcat gagcctgttc
      541 ctggctcagc gtgaagtcgc tcgcattcgt ggccgcaccc tgctgatcga gcatggcttt
      601 tgccgcatcc ggtgtcagtt cctgaccgct cgccagggct gcgccggtca aaacaagaag
      661 accaagggcc caacgaaaga tcctgattcc gtccacagga atctcctttg cgttgttggg
      721 tgtagtgacg aagtggactt atcgtacgac gaaacgaggc cgtcgcgacc gaagaaacga
      781 agcgctatca aaaaaagagt gaaggacaga aagaaaattt atcgggcaaa gaaatgggcc
      841 agctcgggac tcgaaacatg gcgccgaagg cgcgcccgcc gcgacggcca ctcgctcacc
      901 ctcgtgacgg gtgcaccttc agaatctctc tcccgatcac catgcgctga atctcgctcg
      961 tcccctcgcc gatggtgcac agcttcacat cgcgatagaa cttctccacc gggtagtcct
     1021 tgatgaaccc atacccgccg tgcagctgga ccgcctcatt gcagatccgc accgccacct
     1081 ccgacgcgta cagcttcgcc atcgccgatt cctgcgtgac acgcttgccc gcgtccttca
     1141 gcaccgccgc ccgcagcgtc agcagccgcg ccgcatccag ttccgtcgcc atgtccgcca
     1201 gcttccactg gatcccctga aactccgcaa tcgccttccc aaactgcttg cgctgcttca
     1261 cgtacttcac gctcgcatcg agcgcgccct ttccaatccc cagcgacaga gccgcaatcg
     1321 agattcgccc gccgtcgagc gtcctcatcg cgtccacgaa cccctcgccc tcctgcccca
     1381 gcagattctc cgccggaatc gcgcagtctt caaagatcaa ctcactcgta tccgaagccc
     1441 gcaggcccag cttgttttcc ttcttgcccg cacgaaaccc cggcgtgccc ttctccacga
     1501 tgaacgccga cagcccgtgc gttccttcct ccttattcgt caccgcaata atcaccgaca
     1561 catccgcata gtgcccgttc gtgatgaacg tcttcgtgcc gttcagcaca tagtgatcgc
     1621 ccttccgcac cgccgtcgtc cgcgccgccg acgcatccga ccccgacccc ggctccgtca
     1681 atccccacgc gcccaggtgc tctcccgtcg tcagcttcgg caggtacttc ttcttctgcg
     1741 cttccgaacc cgccaggaag atatggttcg tgcacagcga gttgtgcgct gccacaatga
     1801 tcccgatcga cccgtccacc gccgacagtt cctcgatcgc cagcatgtag tcgacatacc
     1861 ccagccctgc gccgccgtat tccgtcggaa aaatcacccc cagcagtccc atccgcccga
     1921 gctgcttcac gacctccatc gggaactccg aggcttcatc ccagcgcagc acgttcggtg
     1981 caatctcgcg tgcagcgaaa tcgcgaa
//
LOCUS       DASZYN010000514         3251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_27703, whole genome shotgun sequence.
ACCESSION   DASZYN010000514 DASZYN010000000
VERSION     DASZYN010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27703"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VHE33_13210"
     CDS             <1..699
                     /locus_tag="VHE33_13210"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HVT98455.1"
                     /translation="NLHWPDFDLKKVIESKMNLPVKMENAATAALLAELTFSRMDGTR
                     DAVLVTISEGVGTGVFANGQLITGNHGLAGEFGHILVDPTGPRCACGQNGCWETFSSC
                     TAALRDYRELEPKARAIDYPELLNLAEEGNPHAVQVLEKQALWIGRGLRMIMAALSPS
                     TILITGEVSPAWHRVGPIIEKEAARLALAGTPPVIRPGHEGEVARLRGAAALVFQRGI
                     PQEKGMPAAISGRA"
     gene            801..>3251
                     /locus_tag="VHE33_13215"
     CDS             801..>3251
                     /locus_tag="VHE33_13215"
                     /inference="COORDINATES: protein motif:HMM:NF019130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HVT98456.1"
                     /translation="MTDTLYHRRSVLVSTTVFAVLLAAVSLHAEDGSQGWLRYAPPVC
                     SNIPASYEKMPAALVNLDTSPQSAFAHNEILRGIRSMFARTLRIETKLPTYDDAWVLG
                     TTAEIRATFPEYRVIAPGPEGFSISILTAHGHQYWIIAGADARGILYGAFHVLSGIAR
                     GVSFASMQGTESPAAPVRWVNQWDNLNGTIERGYGGRSIFFDNGQIRSNLTRAGEYAR
                     LLASIGINGCTINNVNADPKLLTPEMIHEVARIADVFRPWGVRLSMSVPIDAPQVLGG
                     LDTFDPGDPKVAAWWRAKFDEVYAAIPDFGGIEVKADSEGQPGPSKYGRTPVDAANMF
                     AAALKPHGGIVVYRAFVYNHHLDWNDAKADRARAAYDIFHPLDGKFSDNVIVQIKNGP
                     IDFQVREPVSPLFAGLHDTNEAIELQITQEYFGQAHHLVFLPTQWKEALDFDLHAENR
                     STPVKDIIDGKSFHRPLGGYVGVANVGMDTNWMGHPMSMANLYGYGRLAWNPNTTAES
                     IAQDWTRLTFSNDPKVVSTVTRMLLTSWPIYEHYTGPLGLQTLTNITGPHYGPAPQSQ
                     EHNGWGQWIRADGPDAKTGGVGMDRTKTGTGYIDQYPPAIAAMYTSLKTCPDDLLLFM
                     HHVPYTYKLHTGKTVIQTIYDDHYLGAQEAAGFVTQWKSLDGLIDPERYDKVLGLLEY
                     QAGHAIVWRDAVDRWFEKMSGIADDQRRIDHDPNRITASQMQLDGYTPVDVDPWETAS
                     GGKAYVCNRPQGCSASVRVDRSAGWYSVAVQYFDYRRGASSLALKLNQQPIATWTADN
                     SLPGEASNGDTSTRFTLHG"
BASE COUNT          643 a         1147 c          872 g          589 t
ORIGIN      
        1 aatctccact ggcccgactt cgatctgaag aaagtcatcg agtcgaagat gaacctcccg
       61 gtcaaaatgg aaaatgccgc aaccgccgca ctactggcgg aactgacgtt ctcgcgcatg
      121 gacggcaccc gcgatgctgt gctcgtcacc atttcggaag gcgtcggcac gggcgttttc
      181 gccaacgggc aactcatcac cggcaaccac ggtctcgccg gtgaattcgg tcacatcctc
      241 gtcgatccca cagggccacg ctgcgcgtgc ggccagaacg gatgctggga gaccttctcc
      301 tcctgtaccg ccgcgttgcg cgactaccgg gaactcgagc ccaaagcccg cgcgattgac
      361 taccccgagc tgctcaatct cgccgaggag ggcaatccgc atgcggtgca ggttcttgag
      421 aagcaagccc tctggattgg ccgcggattg cgcatgatca tggctgccct ctcgcccagc
      481 acgattctga tcaccggcga agtctcgcct gcctggcatc gagtcggtcc cattatcgag
      541 aaagaggccg cccggctcgc tctggccgga acgccgcccg tcattcgtcc cggtcatgag
      601 ggcgaggtcg cgcgtcttcg tggcgcggcc gcccttgttt tccagcgcgg aattccgcag
      661 gagaagggga tgccggcggc aatatccggc agagcgtaaa ccggcaccgg gccggtccgg
      721 gtctcgttca gcacaattcg ttcagaacgc gaacaaaaga ggccgggttc tgcgacaatg
      781 acccttctgt cgctcccgac atgaccgaca ctctctacca tcgccgctct gtgctcgtct
      841 ccaccaccgt tttcgcggtc ctgctcgcgg ctgtctcgct gcacgctgaa gacggctccc
      901 agggctggct ccgctacgcg ccaccggtat gcagcaacat cccggcgagc tacgagaaga
      961 tgcctgcggc tctcgtgaat ctcgacacct cgccgcaatc cgccttcgcg cacaacgaaa
     1021 tcctccgcgg tattcgctcc atgtttgccc gcaccctgcg catcgaaacc aaactcccca
     1081 cctatgacga tgcctgggtc ctcggcacca cggccgaaat ccgcgccacc tttcccgagt
     1141 accgcgtcat cgcgcccggc cccgaaggtt tctccatctc catcctcacg gctcacggcc
     1201 atcaatactg gatcattgcc ggcgctgatg ctcgcggcat tctctacggc gccttccacg
     1261 tcctctcggg catcgcgcgc ggcgttagtt tcgcatcgat gcagggcacg gaatcgcccg
     1321 ccgccccggt tcgctgggtc aatcagtggg acaacctcaa tgggaccatc gagcgcggct
     1381 acggtggccg atccatcttc ttcgacaatg gccagattcg ctccaacctc acccgcgccg
     1441 gtgagtacgc gcgtctgctg gcctcgatcg gaatcaacgg ctgcaccatc aacaacgtca
     1501 acgccgatcc gaaactgctc acgccggaga tgatccacga ggtcgcgcgc atcgccgacg
     1561 tcttccgccc gtggggcgta cggctttcca tgtccgtccc catcgatgcg ccgcaggttc
     1621 tcgggggcct cgacaccttc gatccgggcg atcccaaggt cgccgcctgg tggcgcgcga
     1681 aattcgatga agtctatgcc gccatccccg acttcggcgg gatcgaggtc aaggccgatt
     1741 ccgaaggcca acccggccct tcaaaatacg gacgcacccc tgtcgacgca gccaatatgt
     1801 tcgccgccgc gctcaaaccc cacggcggca tcgtcgtcta tcgcgccttc gtctacaacc
     1861 accacctcga ctggaacgac gctaaagcag atcgcgcgcg cgccgcgtac gacatcttcc
     1921 atccgctcga cggaaaattc tccgacaacg ttatcgtgca gatcaagaac ggccctatcg
     1981 acttccaggt gcgcgagccc gtctcgccgc tcttcgctgg tctgcacgac accaacgaag
     2041 ccatcgagtt gcagatcacg caggaatatt ttggccaggc acaccatctc gtcttccttc
     2101 cgacacagtg gaaggaggcc ctcgacttcg atctccatgc cgaaaatcgc tccactccgg
     2161 tcaaagacat aatcgacggc aaatcctttc atcgtccgct cggcggctac gtcggcgttg
     2221 ccaacgtagg aatggacacg aactggatgg gccaccccat gtcgatggcc aatctctacg
     2281 gctacggacg gctcgcctgg aatcccaaca cgaccgcaga aagcattgcc caggactgga
     2341 cgcgcctgac gttctccaac gacccgaagg tcgtctccac cgtcacgcga atgctgctca
     2401 cctcctggcc catctacgag cactacaccg gcccgctcgg cctgcagacc ctgaccaaca
     2461 tcaccggacc ccactacggc cccgcgccgc agtcgcagga gcacaacggc tggggccagt
     2521 ggattcgcgc agacggcccg gacgcgaaaa cggggggtgt cggcatggac cgcacgaaaa
     2581 ccggtaccgg ctacatcgat cagtacccac ccgccatcgc cgcaatgtac acgtcgctga
     2641 aaacctgccc cgacgatctg ctcctcttca tgcatcacgt gccgtacacg tacaagctgc
     2701 acaccggcaa gaccgtcatt cagactatct acgacgatca ttacctcggc gcacaggagg
     2761 ccgctggttt cgttacacaa tggaaatcgc tcgacggcct catcgatccc gagcgctatg
     2821 acaaagtgct tggtctgctc gaataccagg ccggccacgc catcgtctgg cgcgatgccg
     2881 tcgaccgctg gttcgaaaag atgtccggca tcgctgacga tcagcgccgc atcgatcacg
     2941 atcccaatcg catcacagca tcgcaaatgc agctcgacgg ctacacaccc gtcgatgtcg
     3001 atccctggga gacggcctcc ggcggcaaag cgtacgtttg caatcggccg cagggatgct
     3061 cagcttccgt gcgcgtcgat cgctctgcag gttggtacag tgttgccgtg caatatttcg
     3121 actatcgccg cggcgcatcc agcctggcgc tgaaactcaa tcagcagccg atcgccacat
     3181 ggaccgcaga caatagtctt cccggcgaag cgtccaacgg cgacacatcg acccgcttca
     3241 ctctgcatgg c
//
LOCUS       DASZYN010000515         1889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_927186, whole genome shotgun sequence.
ACCESSION   DASZYN010000515 DASZYN010000000
VERSION     DASZYN010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927186"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1397)
                     /locus_tag="VHE33_13220"
     CDS             complement(<1..1397)
                     /locus_tag="VHE33_13220"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVT98457.1"
                     /translation="MLKRIRAALTIAVLFTSAFCFAQNTNSGDIRGTVTDATGAVIPG
                     VTVTIQDVDKGTTRTFTTDGAGLYDTGSIVPDHYLLTFSKSGFKTLVRGPILLEVGVD
                     GISVQLEVGNQEVKIEVHTDVPLLDTESGAQEQTLSLKTMEKLPQVGADWQNFIWLQP
                     GAAGAPENSSSAVQPNAGQTAVNGNLPFETVLADGATTTLPMSQNADVTIFETTSEVK
                     ISATAFSAQYGVGDVVYNQITKGGTDQFHGAGYEYFQNNALNAAPYAFGQDATVPFLR
                     YNNFGFNVGGPVLRKKMFFFFDYDKTINNGGASNGFITVPTQAFLSGDFSGSGLPDLY
                     DPTTQTIQQTGTYTYTTSFGAYTQTCPCVIRKTFKSEYGTNAIPAALMDQVSKATQAF
                     YPKANTAGQVSGGITQNNYFYNTPGTNPFTKFFGRLDYDITPSNRLTLSETESDNPAT
                     YLNQGICPINCQHGDV"
BASE COUNT          347 a          500 c          605 g          437 t
ORIGIN      
        1 acatctccgt gctggcaatt gatgggacaa ataccctgat tcaggtacgt ggccgggttg
       61 tcgctctcgg tctccgacaa ggtcagccga ttcgaaggtg tgatgtcgta gtcgaggcgt
      121 ccgaagaact tcgtgaatgg attcgtgccg ggcgtgttgt agaagtagtt gttctgcgtg
      181 atgccgccgc tgacttgtcc ggcggtgttc gccttcggat agaaggcctg cgtcgctttg
      241 gaaacctggt ccatcagagc agcgggaatc gcgttggttc catactccga tttgaaggtc
      301 ttgcggatga cgcacgggca ggtctgggta taggcgccga acgacgtcgt gtaggtgtag
      361 gttccggtct gctggatggt ctgcgtcgtg ggatcgtaca gatcgggcag gccgcttccg
      421 ctgaagtcgc cggaaagaaa tgcctgtgta ggaacggtga tgaagccgtt ggaagctccg
      481 ccgttgttga tggtcttgtc gtagtcgaag aagaagaaca tcttcttgcg caggacgggg
      541 ccgccaacgt taaagccgaa gttgttgtag cggaggaacg ggactgtggc atcctggcca
      601 aaggcatagg gcgccgcatt cagggcgttg ttctggaagt actcgtaacc ggcgccgtgg
      661 aactggtcgg tgccgccttt ggtgatctgg ttgtagacga catcgccgac gccgtactgc
      721 gccgaaaatg cagtggcgct gatcttgacc tccgacgtcg tttcgaagat ggtgacatcg
      781 gcgttctggc tcatgggcag cgtcgtcgtg gcgccgtcgg ccaggaccgt ctcgaagggc
      841 aggtttccgt tcactgcagt ctggcccgca ttgggctgca ccgcgctgct ggagttctcg
      901 ggggctcctg ccgcgcctgg ctgcaaccag atgaaattct gccagtccgc gcccacctgg
      961 ggcagcttct ccatcgtctt caggctcagg gtctgttcct gcgctccgct ttcggtatcc
     1021 agcagcggga cgtccgtgtg gacttcaatt ttgacttcct gattgcccac ctcgagctgc
     1081 acgctgatgc catcgacgcc gacctcgaga aggatggggc cacgaacgag ggtcttgaaa
     1141 ccggacttgc tgaaggtcag caggtagtga tcggggacga tggatcccgt gtcgtacaga
     1201 ccggcgccat cggttgtgaa ggtgcgagtg gtccccttgt caacgtcctg aatggtgacg
     1261 gtgacccctg ggataaccgc tccggttgcg tccgttacag tgccgcgaat atcgcccgag
     1321 ttcgtgttct gcgcgaaaca gaatgcgctc gtgaacagaa ccgcgatcgt cagagcagca
     1381 cgaatgcgtt tcaacatact gtcctcctgt gaaagtgctt ctcgttcttg ctgttcgccg
     1441 gtcgcgagtc gcgccggctg ttttctgagc gggctgctaa aagctgtggg ccgggataga
     1501 acgctctcag aatcgccgtt tgtttccaga caaccgtttg tttacaggga tgcagtcccg
     1561 ggccggcatt catgagacca aaatggaagt ggcgttgtca aggttacttt atgatttttt
     1621 cttcaagaca aacgttttgt cttgaattgg ccggcgcgcg tgacgaattc cggaagcgcg
     1681 gacccgatgg ggctggttgg gaaaaggatc tggccggaag ctccgaaatg cggaaacacc
     1741 cggagtttca acactccaca cctgttccgg tgccgcgcct gctacggcag aacacgcgag
     1801 aggatcttcc gcgacgggaa gccgttgcta tccatatacg cccccggcgg cttttccgac
     1861 gcagttgcgc agttctggct gattgggag
//
LOCUS       DASZYN010000516         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_667129, whole genome shotgun sequence.
ACCESSION   DASZYN010000516 DASZYN010000000
VERSION     DASZYN010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667129"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /gene="rlmD"
                     /locus_tag="VHE33_13225"
     CDS             <1..1166
                     /gene="rlmD"
                     /locus_tag="VHE33_13225"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HVT98458.1"
                     /translation="DKGGFARAELQSIFEASPARVPPPCPYFGRCGGCHYQHAAYPAQ
                     VEMKLTILRESLERAHLRDIPGIQPITAEPFGYRNRVRLHVQRNPFSLCYKFRNSHDN
                     LPVDACPIAAPILERAVQTLNRDTTAAPFAAFVTEVEFFTDPAESALLISLWADRPPN
                     AAKQLAEKTYLSLRQMFPEVQGVGLFAAEKRRPTNRLLAHAGSDSLFYAAAKASYRVS
                     LGSFFQVNRFLIESLVLLVTEGETGSVAWDLYAGVGLFSATLARSFSSVTAVESSPSA
                     VRDLRENLRNTKHRIVAAETAAFLRQTETQRGPCPDLVVVDPPRAGLGPEVTKALGKL
                     RPRKITYVSCDPATLSRDLAALVQFGYRLNQIHLLDLFPQTFHLESVVHLSLD"
     gene            1191..>2264
                     /locus_tag="VHE33_13230"
     CDS             1191..>2264
                     /locus_tag="VHE33_13230"
                     /inference="COORDINATES: protein motif:HMM:NF024956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HVT98459.1"
                     /translation="MAASLAIPESAVRTGGAHPRTPHARFAILAAPMLFIAVFFAGGT
                     TLSCQFWLLPSHLLAALLLLFPVTAVSALVAPRIAPIAAAFLGVLLGLFCAEVQPHLP
                     ASTPLEDIAFSTPASTPAVRHARIATTHVVFGDVIRTTAIRRIDTYAPYSDVLRHERS
                     QQIDLRVATVDGSPLPAPEGLRLTLYAPADASFPQIACGALFMGSAALHTEERFLDPD
                     VWDASRWLRQQGIAALGSAHADQATITPARHRPELICRIRSFQQAASERLVSLADAPG
                     MNRFPAYLRVSHDDAVMLTAMLTGDRTLLSHRLRVGFERTGSFHLLVVSGMHLAIFSG
                     LVFFGARRVRLPRLPASLVTIGLS"
BASE COUNT          351 a          815 c          584 g          514 t
ORIGIN      
        1 aggacaaggg tggtttcgcc cgggcggaac tccagtccat cttcgaggcc agtcccgcac
       61 gcgtgccgcc gccttgtcct tactttggcc gctgcggagg ttgccactat cagcacgccg
      121 cctaccctgc gcaagttgaa atgaagctga ccatcctccg cgaatcgctc gaacgcgctc
      181 atctccgcga catccccggg attcaaccca tcaccgctga gccgttcggc tatcgcaacc
      241 gcgtacgcct tcacgttcag aggaatcctt tctctctctg ctacaaattc aggaactccc
      301 atgacaatct gcccgttgac gcctgcccga ttgcagcacc aatcctcgag cgcgccgttc
      361 agactctgaa tcgcgatacc acggccgcgc cttttgccgc atttgtaact gaggtggagt
      421 tcttcacgga tcccgcggaa tccgccctgc tcatatccct ttgggcagac cgccccccga
      481 acgctgcgaa gcaattggcc gagaagacgt atttgtcgct ccggcaaatg ttccctgaag
      541 tccagggggt tggcttgttc gcggcggaaa agcgccgacc cacaaaccgt cttcttgctc
      601 acgcgggctc cgactctctt ttttatgcgg ctgccaaagc ctcctatcgg gtcagtctcg
      661 ggtcgttctt ccaggtcaat cgctttctga tcgaatccct ggttctcctg gtcaccgaag
      721 gcgaaaccgg ctccgtggcg tgggacctgt atgccggcgt tggactgttc tccgccaccc
      781 tggcgcggtc tttttccagt gtgacagccg tcgaatcgtc gccctccgcc gtgcgcgacc
      841 tccgcgaaaa tctgcgaaac acaaagcacc ggatcgttgc cgccgaaacc gcagcctttc
      901 ttcgtcagac tgagacgcag cgcggtccct gcccggacct cgtcgtggtc gatccacccc
      961 gtgcgggcct gggtcccgag gtcacaaaag ctctgggaaa actccggccg cgcaagatta
     1021 cctacgtttc ctgtgatccg gccacactca gtcgcgacct cgctgcgctc gtacaatttg
     1081 gctatcgcct caatcagata catctgctgg acctgttccc gcagaccttt catctcgaga
     1141 gcgtggtaca cctctcgctg gactgaactt cccccgaggc gtgctttccg atggccgcct
     1201 ccctcgccat tccggaatcc gcagtccgca cgggcggggc tcaccccaga accccgcacg
     1261 ctcgattcgc aatcctcgcg gcgcccatgt tgttcattgc cgtcttcttc gccggcggta
     1321 ccacgctctc gtgccagttc tggctcctcc cttctcatct gctggccgcc ctcctgcttc
     1381 tctttcctgt aacggcggtc tccgcccttg ttgcaccgcg aatcgccccg atcgccgctg
     1441 ctttcctggg cgttcttctt ggtctcttct gcgccgaggt gcagccgcat ctgcctgctt
     1501 cgacgccatt ggaggacatt gccttttcca ctccagcttc cactccggcg gtccggcacg
     1561 cgcgcatcgc gacaacccac gttgtcttcg gcgacgtgat tcgcaccacg gccatccgcc
     1621 ggattgatac ctatgctcct tattccgacg ttctgcgtca tgagcgcagc cagcaaatcg
     1681 atctgcgtgt cgccaccgtc gacggcagcc ctcttcccgc ccccgaggga ctccgcctca
     1741 ccctctacgc tcccgcggat gcgtcattcc cacaaatcgc ctgtggagca ctcttcatgg
     1801 ggtcggctgc gctgcatacc gaggagaggt tccttgatcc cgacgtatgg gacgcttcca
     1861 ggtggcttcg tcagcaggga attgccgcac tggggagcgc ccatgccgat caggcgacca
     1921 tcacccctgc cagacaccgt cccgagttga tctgccgtat tcggtcattc cagcaggccg
     1981 ccagcgaacg cctcgtcagc ctcgccgacg cgccaggcat gaaccgtttc cccgcatatc
     2041 ttcgggtttc ccacgatgat gctgtgatgc tcaccgcgat gctcacgggg gatcgcaccc
     2101 ttctcagcca tcgactgcgc gtcggcttcg agcgcaccgg ctcgtttcac ctgctggtcg
     2161 tttccggcat gcacctggcc attttttctg gcctcgtttt ctttggcgcc cggcgagttc
     2221 gtcttccgcg cctcccggcg tctcttgtga ccatcggcct ttcc
//
LOCUS       DASZYN010000517        10958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_402241, whole genome shotgun sequence.
ACCESSION   DASZYN010000517 DASZYN010000000
VERSION     DASZYN010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 10958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10958
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402241"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VHE33_13235"
     CDS             <1..750
                     /locus_tag="VHE33_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98460.1"
                     /translation="VPVWWGNRLLFLAVAGTAAAVWKWRKKCLAPFLLLWLPLPFYAY
                     SVAYGSVPIFIPLWWPHSFYNTRYGMEMLPIFALSLGFLAAFLIDTTEKRWPRYAPLI
                     LLAGIVLIVLDCSALIRSTPLVLQEAIANSRTRIPFEQAYARALEQLPPQGRILVWTS
                     DHVGAFERAGIPLRRTINETDYYQWKPALKRPAQSAPFVIAADHDAVAQAVAEHPEGL
                     TLLNIVCSTDQPCVRFYRSNAPTSGDRIRPK"
     gene            797..1594
                     /gene="dapF"
                     /locus_tag="VHE33_13240"
     CDS             797..1594
                     /gene="dapF"
                     /locus_tag="VHE33_13240"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HVT98461.1"
                     /translation="MRFAKAHACGNDFLIVEGPADATLAKRLCERHTGIGADGVEFLE
                     TTSPQSGRIRLVNADGSIAEISGNGTRCAAAWIAHQSGSGPGAIINIDTDAGPRACTI
                     VSANQNEYEIAAGMGVPDVRPTQLTLSDKTIVHGVQVSMGNPHFVVVVHDENFRAHAR
                     GWTELGREICFHPQFPQQTNVEFVRMIRGDEVEIRIFERGVGPTTSSGTGTCASAAAI
                     LAHCGGRSPLVVHAPGGSQKVEWTGATAEMILTGPAILVAAGEVVLA"
     gene            1587..2543
                     /locus_tag="VHE33_13245"
     CDS             1587..2543
                     /locus_tag="VHE33_13245"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HVT98462.1"
                     /translation="MPDRFLKPAAVAPGARIAVVSLASSARADRITRGMDCLRALGYD
                     VVASEHAFGKHPPYFSGSVEDRLADLHNAFADPSVSAIICTRGGYGSNYLLDGLDLDL
                     IRSHPKPLFAYSDMTAMQTWILDQTRLVSFHGPMVAADFALDKGGIHIESFRAAIGGG
                     LVRVGAAEGLRVLRSGTARGILYGGCLSLLTAALGTHYAPQTEGKLLFFEDVGAKPYQ
                     IDRMLRQMILAGKLKGARGFVFGEMLECGSRGADPDLLQQVILRLLEPFNVPIAIGLR
                     SGHVSGGNVTLPFGTEAELLLEGDQPLLHVLDRAVTVPRNGR"
     gene            2533..3966
                     /gene="mpl"
                     /locus_tag="VHE33_13250"
     CDS             2533..3966
                     /gene="mpl"
                     /locus_tag="VHE33_13250"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR01081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HVT98463.1"
                     /translation="MADKKHIHLIGICGTAMASLAGLLQLQGHRVTGSDQAAYPPMSD
                     LLRGLGIPIAEPFSEANLQPAPDLIVVGNAISRGNPEVEFMLDQHIPFTSLARMIHDE
                     FIRDHESLVVAGTHGKTTTTSMLAWIYEAAARRTPELAPSFLIGGVAENFGTSFRLSH
                     GQPFIIEGDEYDTAFFDKGPKFMHYFPNGLILTHVEFDHADIYIDLEAVKTAFKRLVN
                     LVPRRGRIVAFDGSENVSECVNHAFCSVERYGFNANSTWRLTDLRFEGGASHWRVLRS
                     GKPWAELTLPMAGEHNALNATAAAALAAGQGIHSEPIIEALATFRSVRRRLEVRAQID
                     GITIIDDFAHHPTAIRETLRALRSAYPGRRLWAVLEPRSNTLRRNVFERELVESLSLA
                     DRIVVAGVFHSEKIPAKERLHPEVVVSALQQSGRDAALLENADAIVTNLARRLEPGDV
                     VAILSNGGFDGIYEKLPASLRQRNLQT"
     gene            3970..4680
                     /locus_tag="VHE33_13255"
     CDS             3970..4680
                     /locus_tag="VHE33_13255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVT98464.1"
                     /translation="MLATIALLGIWILLGLPVAVIAIPWTVVTRDILPLYRAAIVVVR
                     FGLWCAGIRVEAAWREPLDSRRRYIFLANHVSNLDPPILIPLLPLPVAVVLKRSLMRI
                     PVLGWAMKLAGYIPVDRDGRVESAVESSEAAKRTLRAGSHILSFPEGTRSHDGRLQPF
                     KKGPFYMAEESGALVVPVSIYGTERMMKKGSVKIFPATAHVVFHAPIRPQNYASRDEL
                     MAAVHSIVASGLPEWMRD"
     gene            complement(4757..5461)
                     /locus_tag="VHE33_13260"
     CDS             complement(4757..5461)
                     /locus_tag="VHE33_13260"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HVT98465.1"
                     /translation="MAFRFETEKEYEDVKRRRSGTASGSTDGSDTEIALGTRSLLAIF
                     FGLVLICAVFFGLGYSVGRSSGTRAAAQPPMETATAAADSRIAKPSPDQSLTPVETPV
                     DNGSPNTENPAVTQPGSGSAPAAASASSQTPVNPFQSTGQSPATSAATSSGTLQNAGA
                     TLPASFMVQVAAVRVPQDAQILVDALKKHGYSAVVRNEPQDQLLHIQLGPFPARSDAI
                     AMRAKLLTDGYNAVVK"
     gene            complement(5489..6271)
                     /locus_tag="VHE33_13265"
     CDS             complement(5489..6271)
                     /locus_tag="VHE33_13265"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HVT98466.1"
                     /translation="MFSVPALVVIVIVVLYLLNSIKILREYERGVIFRLGRALPVPKG
                     PGLILVFRPLDQMVRVSLRQEVMEVPPQDVITKDNVTIKVNAVITMRVLDPAKAVIEV
                     SNYIYQTSQFAQTTLRSVLGEVELDGLLSHRDVLNQRIQTIIDQRTEPWGVKVVSVEV
                     KQVDLPDTMLRAMARQAEAEREKRSKIINAEGEFMAAQKLVEAANLLAEQPVTLQLKY
                     LQTLTDIGVEKNTTIVFPLPMELISLLNRFIPASAAAPPETK"
     gene            complement(6416..7291)
                     /locus_tag="VHE33_13270"
     CDS             complement(6416..7291)
                     /locus_tag="VHE33_13270"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98467.1"
                     /translation="MTSSVKIIECPRDAWQGLTKPIPPEVKADYLRTLVAAGFTHIDA
                     ASFVSPKAVPQMADSEQTLALLDPPDDVEVIGIVVNPKGAERAIATEAVSTLGFPYSI
                     SQQFLRRNQNQTPEESLEALEAVGQAAFQAGLSVVVYISMAFGNPYDDPWSLDEVIAA
                     CDLLTDSGFTQISLADTVGLATPQQVSRLTAAVLDAVDDSIEIGVHLHARTEDAAAKI
                     AAAYNAGCRRFDMAIGGFGGCPFAQDALVGNIATEIALAELRRLGADLPRLQPLDGLR
                     AAATAIETRYSPPVQ"
     gene            complement(7390..7794)
                     /locus_tag="VHE33_13275"
     CDS             complement(7390..7794)
                     /locus_tag="VHE33_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98468.1"
                     /translation="MPSSSAATARNKRGSRKTLARGWWKGLWPLLIGIIITPFTVRLA
                     GVLALEGPKAFALLYPWFDVLRSRVLHSPADLVGHAYEYMLYLQFPLYGLLMTFTFRQ
                     GRHLRAFIIGLLAHFTGLMLVVVLAWLGQMPQ"
     gene            8013..8711
                     /locus_tag="VHE33_13280"
     CDS             8013..8711
                     /locus_tag="VHE33_13280"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HVT98469.1"
                     /translation="MHRTIDPGILYFGTPVVLVSSLNEDGSPNLAPMSSAWWLGRRCL
                     LGFGARSKTPQNIIRTGECVLNLPSPAQAGMVDRLALTTGSNPLPEHKAKWGYRYEPD
                     KFGTAGMTPVDSTMVQAPRAMECPVQLEATLRSVHRIGEEDEALRGRLLGLEVHIERI
                     HVEEGLLATGKLNHIDPDKWSPLIMSFQQFYGLGRRLQESRLASIPESNYRMASVTPV
                     AEPFTRLTFAESPD"
     gene            complement(8686..10422)
                     /locus_tag="VHE33_13285"
     CDS             complement(8686..10422)
                     /locus_tag="VHE33_13285"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HVT98470.1"
                     /translation="MTDSTDSKTASPEKLNTRLTSKLDAASPRIRANAEKMTALLDQI
                     RQQENEIRQGGGAKSIDAQHAKQRRTARERLDLLLDPDTHFFELGLFAAFGMYEEWGG
                     APSAGVLTGLGRVAGRLCMIVANDATVKAGAFFPMTAKKVLRAQHIAMENRIPTLYLV
                     DSAGIFLPLQEDVFPDQDDFGRIFRNNAVMSASGIPQITAIMGMCVAGGAYLPVMTDQ
                     VLMTEGSGLFLAGPALVQAAIGQKYSAEELGGATMHAEISGTVDFKEPNDHLCIARLR
                     SLVSKWGHPPRSSFDRTAYDPANDAPRYPASDLRALLDSDPARAAVNNYDMHDLIARV
                     VDRSEFDEYRPGYGRTLLCGYARIGGYAVGIVANQKLPQPQTSHSGEKRVEFGGVIYA
                     ESADKAARFIMDCNQNLVPLIFLHDVNGFMVGRDAEWSGIIRSGAKMVTAVSNSVVPK
                     ISVIVGGSFGAGNYAMCGKAYDPRFIFAWPTARYAVMSGDSAANTLVEIRIRQLERGG
                     RQLSDDEKQELIASIKATYEAQTDCRYAAARLWVDAIIDPAETREALLTALEAVALNP
                     DVPRFNPGILQT"
     gene            complement(10599..>10958)
                     /locus_tag="VHE33_13290"
     CDS             complement(10599..>10958)
                     /locus_tag="VHE33_13290"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HVT98471.1"
                     /translation="TETSGSPYTTNTGAWSVLVDSTGSYVYVANRTAGTISAFLLTNT
                     GALTAVSGSPFATGSLPVALVEDKTKAYIAVVCSGGNSDLQVFSISSTTPGALTSFAT
                     AKTGTDPTSASSLAATF"
BASE COUNT         2056 a         3286 c         3349 g         2267 t
ORIGIN      
        1 gttccagtct ggtggggcaa tcgtcttctg tttcttgctg tcgcaggaac ggcggcagca
       61 gtctggaaat ggcggaagaa atgtctcgcg ccctttctgc tgctgtggct gccgctcccg
      121 ttctacgcgt actccgttgc ctacggatct gtgcccatct tcattccgct gtggtggccg
      181 cactctttct acaacacgcg ctacggcatg gaaatgctgc ccatcttcgc gctttcgctc
      241 ggcttcctcg ccgcatttct tatcgacacc acagaaaaac gctggccccg ttacgccccg
      301 ctgatcctgc tcgccggtat cgtcctgatc gtcctcgatt gctctgccct catccgctct
      361 acgccgctgg tcctccagga ggcgatcgcc aactcccgga ctcgcattcc tttcgaacag
      421 gcttacgccc gtgccctcga acaattgccg ccgcaaggca ggatcctggt ctggacctcg
      481 gaccacgtcg gcgccttcga gcgcgccggg attccgctgc gccgtaccat caacgaaacc
      541 gactactacc agtggaaacc ggccctcaaa cgcccggctc aatcggcgcc atttgtcata
      601 gcagcggatc acgatgctgt ggcgcaagcc gtggccgagc atccagaagg actcacgctg
      661 ctcaatatcg tgtgctcaac ggatcaaccc tgcgttcgtt tctaccgttc aaatgcaccc
      721 acgtccggtg ataggatcag gccgaagtga tccttgcaac cgacaacatc cactcgcgcc
      781 atccggagat tccgtgatgc gctttgcgaa ggcgcatgcc tgcggcaatg acttccttat
      841 tgtcgagggt cccgcagatg ccaccctggc aaagcgtctc tgcgaacggc acacgggtat
      901 tggcgccgat ggtgttgagt ttcttgagac gaccagtccg caaagcggcc gcatccgttt
      961 ggtgaacgcc gacggctcca ttgcggagat ttccggcaac ggcacgcgct gcgccgccgc
     1021 atggatcgcg caccagtccg gctccggacc gggggcgatc atcaacatcg ataccgatgc
     1081 aggccctcgc gcctgcacca tcgtgagcgc gaaccagaac gaatacgaga ttgccgcggg
     1141 catgggcgtg cccgatgttc gcccgaccca gcttacgctc tccgacaaaa ccatcgttca
     1201 cggagttcag gtatcgatgg ggaacccaca cttcgtcgtc gtggtgcacg atgaaaattt
     1261 tcgggcacac gcacgcggct ggactgaact gggccgcgag atttgctttc atccgcaatt
     1321 tcctcagcag acgaacgtcg aattcgtacg catgatccgg ggtgacgaag tcgagattcg
     1381 catctttgag cgcggggtcg gcccgacaac atcctccggt accggaacct gcgccagcgc
     1441 ggcagccatc cttgcgcact gtgggggcag gtcgccgctg gtcgtgcatg cgccaggggg
     1501 ttcccagaag gtggaatgga caggcgcgac tgcagagatg atcctgacgg gtccggcgat
     1561 tctggtcgcc gcaggagagg tggttcttgc ctgatcgatt cctgaaaccc gctgcggttg
     1621 cgccgggtgc gcgcatcgcc gtcgtttccc ttgccagcag tgcccgcgcc gaccgcatca
     1681 cacgcggcat ggattgtctc cgtgctctgg gttatgacgt ggttgcctcc gagcacgcct
     1741 tcggcaaaca ccctccctat ttttccggct ctgtcgaaga ccgcctcgcg gatctccaca
     1801 atgccttcgc ggatccatct gtgtccgcca tcatttgcac tcgtggtggc tacggatcga
     1861 actatcttct ggacgggctc gatctggatc tgatccgttc gcatcccaag cctttattcg
     1921 cgtacagcga tatgaccgcg atgcagacct ggattctcga tcaaacccgt cttgtctcgt
     1981 ttcatggccc catggttgcc gcagatttcg ctctggacaa aggtggcatc cacatcgaga
     2041 gctttcgtgc cgcaataggc ggcggacttg ttcgcgtggg tgcagcggag ggccttcggg
     2101 ttctgcgttc cggcacggcg cgcgggattc tttacggagg atgcctgagc ctgctcacag
     2161 cggctctggg tacgcattac gctccacaga ccgagggtaa gctgcttttc ttcgaagatg
     2221 tgggggcaaa gccctatcag atcgatcgca tgctccgcca gatgatactc gccgggaaac
     2281 tcaagggtgc tcgagggttc gtcttcggcg agatgctcga atgcggatcg cgcggcgccg
     2341 atcccgattt gcttcagcag gtcattctcc gtctcctgga gcctttcaac gtcccgatcg
     2401 ccatcggcct gcgttccggg catgtctctg gcggcaatgt cacactgccc ttcggcaccg
     2461 aagcagaact tctgctcgaa ggcgatcagc cgctattaca tgttctcgat cgtgctgtca
     2521 ctgttccccg caatggcaga taagaagcac attcacctca tcggaatttg cgggactgcc
     2581 atggcttcgc tcgccgggct gctccaactc cagggtcatc gtgttaccgg ctccgatcag
     2641 gccgcgtatc cgccaatgtc cgatctgctc agggggcttg gcatcccgat cgccgagccg
     2701 ttctctgaag ccaatctgca accagcaccc gatctcatcg ttgttggcaa cgccatttcg
     2761 cgagggaatc ctgaagtcga gtttatgctc gaccagcaca tccccttcac gtcgctggcg
     2821 aggatgatcc acgacgaatt cattcgggac catgagtcgc tcgtcgttgc cggaacccac
     2881 ggtaagacca ccaccaccag catgctggcc tggatctacg aggcggcggc gcgacgcaca
     2941 ccggaacttg caccttcgtt tctcatcgga ggcgtcgctg agaactttgg caccagcttc
     3001 cgtctgagcc acggacagcc tttcatcatc gaaggggacg aatacgatac agccttcttc
     3061 gacaaaggcc ccaagttcat gcactatttt cccaacgggc tcatccttac gcatgtcgaa
     3121 ttcgaccatg ccgatatcta tatcgatctt gaggccgtaa agactgcatt caaacgcctg
     3181 gtgaacctcg taccgcggcg cgggcggatc gtcgcattcg acggctcaga aaacgtctcg
     3241 gagtgcgtga atcatgcatt ctgctccgtc gaacgttatg gtttcaatgc gaactctaca
     3301 tggcggctca ccgatttgcg tttcgagggc ggtgcgagtc actggcgcgt cctgcggtcg
     3361 ggcaaaccct gggctgagtt gacactgccg atggcaggcg agcacaacgc actcaacgca
     3421 accgctgccg cggctctggc tgccggacag ggaattcact cggagccgat tattgaggct
     3481 ctggcgacat ttcgcagcgt acgccggcgt ctggaagtcc gcgctcagat tgatggcatc
     3541 acgatcatcg acgatttcgc gcatcacccc acggcgattc gcgagacgct gcgagcgctc
     3601 cgttcggcgt acccgggccg gcgtctctgg gcggtgcttg aaccacggtc caatacgctg
     3661 cgccgtaacg tcttcgagcg cgaactggtg gagagtcttt cgctggcaga tcgcatcgtt
     3721 gtcgcgggcg tctttcactc tgaaaagatt cccgcgaagg agcggctgca tccggaggtt
     3781 gtcgtttccg cgctgcaaca atcgggtcgc gatgcagcac tgctggaaaa tgccgacgcc
     3841 attgttacaa atctcgcccg tcgtctagag cccggtgacg tcgtcgcgat tctgtccaat
     3901 ggcggattcg acggcattta tgagaagcta cccgcatcgc tccgacagcg caatctgcaa
     3961 acctgatcca tgctcgcgac catcgccctt ctcggcatct ggattcttct cggactgccc
     4021 gttgcggtca tcgccattcc ctggacggtc gtcacgcgtg acatcctgcc gctgtatcgt
     4081 gctgccattg ttgtcgtccg cttcgggttg tggtgcgcgg gcattcgcgt tgaggcagcc
     4141 tggcgcgaac ccctggattc caggcggcgc tatatctttc tggcgaatca cgtgtcgaac
     4201 cttgacccgc cgatcctgat tccgctgctg ccgctgcctg tggcggtggt cctgaagcga
     4261 tccctgatga gaattcctgt tcttggctgg gccatgaagc tggccggata tatcccggtt
     4321 gatcgcgatg ggcgtgtaga gagcgccgtg gagagttccg aagccgcgaa gcggaccctg
     4381 agagcaggga gccacatcct gtcctttcct gagggaaccc gttcgcatga cggacggctg
     4441 caacccttta agaaaggccc gttctatatg gctgaagaat cgggagcgct tgtcgtgccg
     4501 gtttccattt atggaaccga gaggatgatg aaaaagggaa gcgtgaagat ctttccggcc
     4561 acggcgcatg ttgttttcca cgcaccgatc aggccgcaaa actatgccag tcgcgatgag
     4621 ctaatggcgg cagtacattc catcgtcgct tccggcctgc ctgagtggat gcgggactga
     4681 ggctggcagt ctgagccggc agcccgattc ggctccgggg cggcgctggt tcgtttaacg
     4741 ggtccgttcg gcccttctac ttgacgactg cgttgtagcc atccgtgagc agttttgccc
     4801 gcatcgctat ggcgtcggag cgagcgggga atgggcccag ctgaatgtgg agcaactggt
     4861 cctggggctc gtttcgcacc accgcggagt atccgtgctt cttcagcgca tcgaccaaaa
     4921 tctgcgcatc ttgcggtacg cgtacggcgg ctacttgcac catgaagctc gccggaagcg
     4981 tcgcacctgc attttgcagc gttccggagc tggttgccgc cgaggttgca ggggactgtc
     5041 cagtgctttg gaagggattg actggtgtct gtgaggatgc tgatgcggcg gcgggggcag
     5101 atccactgcc cggctgcgtt accgctggat tctctgtgtt tggcgaaccg ttatcaaccg
     5161 gcgtttctac cggcgtgagc gattgatctg gcgaaggctt cgcgatgcga ctgtccgctg
     5221 cagcagtagc tgtctccatt ggaggttgtg ccgccgcacg agtcccgctg ctgcgtccca
     5281 cggaatagcc cagcccaaag aacactgcgc agatcaagac gagaccaaaa aagattgcca
     5341 gcagggaccg cgttcccaga gcaatctccg tatccgagcc gtcggtgctt cctgatgcgg
     5401 tccccgagcg ccgccgcttc acgtcttcgt actctttttc cgtctcaaat cgaaaagcca
     5461 taggggacct ctgggagatg agaacccgtt attttgtttc tggtggcgct gcggcgctcg
     5521 ccggtatgaa ccggttgagc agagaaatca gctccatcgg cagagggaag acgatcgtcg
     5581 tgttcttttc gaccccgatg tcggtcaagg tctgcaagta cttcagctgc agcgtgaccg
     5641 gctgttccgc cagcaggtta gcggcttcca ccagcttctg cgcggccatg aactcgcctt
     5701 ccgcgttgat aatcttcgac cgcttctcgc gttccgcctc ggcctgccgg gccatggcgc
     5761 gcagcatggt gtcgggaagg tccacctgct tcacttccac gctgacgacc ttcacgcccc
     5821 acggttcggt acgctggtcg atgatcgtct ggatccgctg attgagaacg tcgcggtgcg
     5881 aaagcagccc atccagttct acctcgccga ggacggagcg cagcgtcgtc tgagcgaact
     5941 gcgaggtctg gtagatgtag ttcgaaacct caatgaccgc ctttgcggga tcgagcaccc
     6001 gcatcgtgat cacagcgttc accttgatcg tgacgttgtc tttggtgatc acgtcctggg
     6061 gcggcacttc catcacttcc tgtcgcagcg acacccggac catctgatcg aggggacgaa
     6121 aaaccaggat gagacccgga cctttcggca ccgggagggc ccggccgagg cgaaagatga
     6181 caccgcgctc gtactcgcgc agaatcttga ttgaattcag cagataaaga acgacaatga
     6241 cgatgacaac aagagctgga acgctgaaca tacgccgggt accttatgcc cggtgtgatt
     6301 ttaccgcagg ggataccgtc aagcccgcat aaaatcaggc cgaatgactc tgtcgtaccc
     6361 gttcgactac ccccgctgga caggccattt caccttgacc acaccgcacc ggaactcact
     6421 gcacgggcgg agaatagcgc gtttcgatgg ctgtggccgc cgcacgaagg ccatcgagag
     6481 gctggagtcg aggcaggtcg gctcccagac ggcggagctc cgcaagggca atttccgtgg
     6541 caatattgcc caccagcgca tcctgggcga aggggcatcc tccgaacccg ccgatcgcca
     6601 tatcgaagcg gcgacagccg gcgttgtagg cggcagcgat tttcgcggcg gcgtcttcgg
     6661 tccgcgcgtg gaggtgtacg ccaatttcga tcgaatcatc caccgcatcg agcaccgccg
     6721 cggtcagacg cgaaacctgc tgtggggttg ccagtcccac ggtatcggca agcgaaatct
     6781 gggtgaagcc cgagtccgtg agcaagtcgc acgcagcgat cacctcgtcg agactccacg
     6841 ggtcgtcgta ggggttcccg aaggccatgg agatgtagac cacgacggaa agcccggcct
     6901 gaaaagctgc ctggccgacc gcttcgagcg cttccaggga ttcttcgggc gtctggttct
     6961 ggttgcggcg caggaattgc tgcgagatgg agtagggaaa gccgagcgtc gaaaccgctt
     7021 cggttgcaat ggcgcgctcg gcgcctttcg gattgacgac gattccgatg acctcaacgt
     7081 cgtccggtgg atcgagcagg gccagcgttt gctcggaatc ggccatttgc ggaactgcct
     7141 ttggggagac gaaagaggca gcgtcgatat gagtgaaccc ggcggcgacc agcgtacgca
     7201 ggtagtccgc cttcacttcg gggggaattg gtttcgttag cccctgccag gcgtcgcgag
     7261 gacactcgat aatcttcacg gacgaagtca tatcaagcgc tgggcagaga aagccgaggt
     7321 ccacttaccc agcaagccgt agggtatcag ggccagggtg tgtcgtggcc ggtcactttg
     7381 ctccggtgat cactgcggca tctgtcccag ccaggcaagc acgaccacga gcatcagccc
     7441 ggtgaaatgc gcaagcagac cgatgatgaa cgcgcgcaga tggcgcccct ggcggaacgt
     7501 gaacgtcatc aggaggccat agaggggaaa ctgcaggtac agcatgtact cataggcgtg
     7561 acccaccaga tctgcgggcg aatggaggac ccggctacgc agcacgtcga accaggggta
     7621 aagcagtgcg aatgccttgg gcccttcgag cgccagaaca cccgccaggc gcaccgtgaa
     7681 gggcgtgatg atgattccga tcaggagcgg ccagaggccc ttccaccagc cgcgggcgag
     7741 agtcttccga gagccgcgct tattgcgcgc tgttgcagct gaggatgaag gcatagttcg
     7801 agaatactct ccgggccgat ggaagccgag cgaaaaacga tggcgaaatg gttacgttgg
     7861 ccagttacgt ttggaggatg tcagtcctcg accgaatctg cagtcttcgc cggatcctcg
     7921 cttcctgagc ctgcggcttt ttcgaatttg cccgcaaggt tcggatagcg gccgttcgat
     7981 atggtgcccg atgctggaga tggaggaaca cgatgcaccg cacgatcgat ccaggaattc
     8041 tctatttcgg aacacccgta gttttggtta gctctctgaa tgaggatggc tcgccaaacc
     8101 tggcgccgat gtcctcagca tggtggctcg gccggcgttg cctgctcggg ttcggagcgc
     8161 ggtcgaagac gccgcaaaac atcattcgca cgggagagtg cgttctgaac cttccttcgc
     8221 ccgcacaggc aggaatggtg gatcggctcg cgctgactac cggctcgaac cccctgcccg
     8281 agcataaggc gaagtggggc taccgctatg agcccgacaa gttcggaacc gcaggaatga
     8341 caccggtgga ttccacgatg gtgcaggcgc cccgcgcgat ggaatgtccc gtgcaactgg
     8401 aagcgaccct ccggtcggtg catcggattg gtgaggagga cgaggcgttg cgcggccggc
     8461 tgctgggttt agaggttcat atcgagcgaa tccatgtgga agagggcctg ctggctaccg
     8521 gcaaactgaa ccacatcgat ccggataagt ggagtccgct cattatgagc ttccagcagt
     8581 tctatggcct cggccggcgg ctgcaggaat cacgcctggc gagtattccg gaatcaaatt
     8641 accgcatggc gtcggtgacg ccggttgctg aaccattcac ccgcctcacg tttgcagaat
     8701 ccccggattg aagcgcggaa cgtccggatt gagcgcaacg gcttcgagcg cggtgagcaa
     8761 agcctcacgg gtttccgccg gatcgatgat cgcatccacc cagaggcgcg cggcggcgta
     8821 ccggcagtcg gtctgcgctt cgtaggtcgc cttgatcgag gcgatcaact cctgcttctc
     8881 atcgtcgctc agttggcggc cgccgcgttc gagctggcgg atgcgaatct cgacgagcgt
     8941 gttcgctgcc gagtctccgc tcatcacggc gtatcgtgct gtcggccagg caaagatgaa
     9001 ccgcggatca taggctttac cgcacatggc atagttgccc gcgccgaacg atccgcccac
     9061 gatcaccgaa atctttggca ccacggaatt cgataccgcc gtcaccatct tggcgccgga
     9121 gcgaatgatc ccactccact cggcgtcgcg gccgaccatg aagccgttta cgtcatgcag
     9181 aaagatgagg gggacgaggt tttgattgca gtccatgatg aaccgggctg ctttgtcggc
     9241 gctttcggca tagatcactc cgccgaactc gacgcgtttt tcgccggagt ggctggtttg
     9301 aggctgcggt agcttttgat ttgcgacgat cccgacagca tagcctccga tccgtgcgta
     9361 tccgcagagg agggtgcggc cgtagcctgg gcggtattcg tcgaactcgc tgcgatcgac
     9421 aacgcgcgcg atcaggtcgt gcatgtcgta gttgttcact gcggccctgg cgggatcgga
     9481 atcgagcagc gcgcgcagat cggaggcggg gtagcgcggc gcatcgttcg cgggatcata
     9541 cgccgtgcga tcgaatgagg accgcggcgg atggccccac ttcgagacca gcgaccgcag
     9601 acgcgcgatg cagaggtgat cgttcggctc cttgaaatcg actgtgccgg aaatttccgc
     9661 gtgcatggtt gcgccgccca gttcttcggc ggaatatttc tgcccaatcg ccgcctgcac
     9721 cagcgccggc ccggcaagaa acagcccgct gccttcggtc atcagtacct ggtcggtcat
     9781 gaccggcaga tacgcgccgc cggcgacgca catgcccatg atcgcggtga tctgcggaat
     9841 gccggaagcg gacataacgg cgttgttgcg gaagatgcgg ccgaagtcgt cctggtcggg
     9901 aaagacatct tcctgcagcg gaagaaagat gccggccgaa tcgacgagat agagggtcgg
     9961 gatgcggttc tccatggcga tgtgctgcgc gcgcaggacc ttctttgcgg tcatcgggaa
    10021 gaaggcgccg gctttcaccg tggcgtcgtt ggcaacgatc atgcagaggc gtcccgccac
    10081 tcgtccgagc cccgtcaata ctccggccga cggggcaccg ccccattcct catacatgcc
    10141 aaaggcggcg aacaggccga gttcgaaaaa atgggtatcg ggatcaagca gcaggtccag
    10201 ccgctcgcgg gcggtccgcc gctgcttcgc gtgctgtgcg tcaatcgatt tcgcgccgcc
    10261 gccctggcgg atttcgttct cctgctgtcg gatttgatcg agcaatgccg tcatcttttc
    10321 agcattggcg cggatgcgag gagaggccgc atcgagcttt gacgtgagtc gggtgttgag
    10381 tttctctggg ctggcggttt tggaatcggt cgagtcggtc atctggggac agatcagggt
    10441 acagggtaag ggggttgggg tacagggggc agaccaacct gcgacgaatg ccgtttatgg
    10501 ctgggatccg gcgttccttc gcccgcatgg tgaactgacc cattcggatc cagctgaatc
    10561 tctgtcgata ggggtcaacg ccccctccga tgcgaaccct aaaacgtcgc ggcaaggctc
    10621 gaagcggagg tcggatcggt tcccgtcttc gcggttgcga aggatgtcag tgcgcccggg
    10681 gtggtgctgc tgatgctgaa aacctgcagg tcggagttgc cgccggagca gactacggcg
    10741 atataggcct tggttttgtc ctccaccagg gcgaccggca gcgagcctgt agcgaaggga
    10801 gagcccgaaa ccgccgtgag cgcacctgtg ttcgtgagga ggaaggcact gatcgttccc
    10861 gcggtacggt tggcgacata gacgtagctt cccgtggaat cgaccagcac cgaccaggcg
    10921 ccggtgttcg ttgtgtaggg tgagccgctg gtttcggt
//
LOCUS       DASZYN010000518         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_279110, whole genome shotgun sequence.
ACCESSION   DASZYN010000518 DASZYN010000000
VERSION     DASZYN010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279110"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="VHE33_13295"
     CDS             <1..1281
                     /locus_tag="VHE33_13295"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVT98472.1"
                     /translation="ALPALETRRVDLRSSIASGTRSVAGGSSLVRKVLIGAEVALTVV
                     LLAGAGLLIRTLAHLESIPPGFDPHNVIAAKASLDDARYHDAAAFDQLLAKSVAAMQQ
                     IPGVENAAVALSAPYETFINDGVQFADGPMAGQEEASSFSYITPQYFAALRIPLLSGR
                     AFTDGDTATTEPVAIVNTAFGKHFYNDANPLGRHFKDGTRTFAIVGVVSDVLDQSNVR
                     RDAPIGVDPIYYVPYTQTSPRLLTIVHMWAQPSWIVRTRGPIAGLTGRMQQALSAVDP
                     GLPFSGFYSMQDLLDQQLQMQRIEVTLLGTLAGLALLLSAVGIYALVSNLVVQRTREI
                     GIRIALGSTLAQAMRQMASSGIAAAVGGVAAGLVCSFFVLRVMKSAIYGVTTWDPVTL
                     ISVPLALITMAAVASLLPALRIARIDPAETLRAE"
     gene            1343..>2540
                     /gene="lptC"
                     /locus_tag="VHE33_13300"
     CDS             1343..>2540
                     /gene="lptC"
                     /locus_tag="VHE33_13300"
                     /inference="COORDINATES: protein motif:HMM:NF018537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HVT98473.1"
                     /translation="MRVTTRGLRRWIVGTAGLLLLVVAGFVIYGRNRFRHIEKDLPGR
                     LGISIQSTSSTITFSQSTQGHTLFTLKAAKQVQMKSGHVLLHDVDITLYGPPGSGRKD
                     RIAGTDFDYDQTHGIIISQGDVEIELQGAGKNVAPSGEDGDANANTMRVKTSGLTFLQ
                     KTGAASTQQQVEFELPRAAGTAVGADYNAKTGVVVLDHDVHITSSTNGKAAVVDATHA
                     TLLRESNQAQLVNARVSYQNEEAGSDQATVNFRRDGTAEEIDAQGHVRMRTDDGATVN
                     AETAHILLDTKSEPTRADLAGGVQYSSARNNATMHGSSGSGTLLFAVINGAGGARTSL
                     RHAEFREDVRFIQNVTGLAKDLRGHAQKEMQAQQLNVEFAPARKGRGFEARKAIAEGS
                     PVITSRE"
BASE COUNT          486 a          787 c          825 g          442 t
ORIGIN      
        1 gcgctgccgg cgctggagac gcgccgggtc gatctgcgca gctccatcgc gtcgggaacg
       61 cggtccgttg ccggcggctc gagcctggtt cgcaaagttc tcatcggcgc cgaagtcgcg
      121 ctgaccgtcg tgctgctggc cggagcaggc ctgctgatcc gcacgctcgc gcacctggag
      181 tcgatcccgc ccggtttcga tccgcacaat gtgatcgcag cgaaggcttc gctcgacgac
      241 gcgcgttatc acgatgccgc cgcgttcgat caactgctcg caaagagcgt tgccgcgatg
      301 cagcaaatcc cgggcgtaga aaacgcagcg gtagctctga gcgcacccta tgaaacgttc
      361 atcaacgatg gcgtccaatt tgccgacggc ccgatggcgg gtcaggaaga agccagcagc
      421 ttcagctaca tcacgccgca gtatttcgcg gcgctgcgca tccctttgct ttccggccgc
      481 gccttcaccg acggcgacac ggcgacaacc gagccggtgg ccatcgttaa taccgcgttc
      541 ggcaagcact tctataacga tgccaatccc ctcggccgcc acttcaaaga cggaaccagg
      601 actttcgcga tcgtgggagt cgtgtcggat gtgctcgacc agtccaacgt gcggcgtgat
      661 gcgccgatcg gcgtcgatcc aatctattac gtgccctaca cgcagacctc gccgcggctg
      721 ctgaccatcg tgcatatgtg ggcgcagccg tcctggatcg tgcgcacccg cgggcccatc
      781 gcgggactca ctggccggat gcagcaggcc ctctccgctg tcgatccggg gctcccgttc
      841 tccggtttct actccatgca ggatttgctc gaccagcagc tacaaatgca gcgcattgaa
      901 gtgacgctgc tgggaactct ggccggcctt gcgttgctgc tgtcggccgt gggcatctac
      961 gctctggttt cgaacctggt ggtgcagcgg acgcgcgaga tcggcatccg gatcgctctg
     1021 ggatcgacgc ttgcgcaggc catgcggcag atggcctcct ccgggatcgc agccgccgtc
     1081 ggcggtgtag ccgcgggcct ggtctgctct ttctttgttc tgcgcgtgat gaagagcgcg
     1141 atctacgggg tcacgacctg ggatcccgtg acactgatca gcgttccgtt ggcgctgatc
     1201 acgatggcgg ctgtcgccag cctgctgccc gcgttgagaa tcgcacggat cgatccggcg
     1261 gagacgttgc gagccgagtg acaggggctg ccgggggcgc gtgcgaggca ggaccggcgc
     1321 gaatgagaaa atagatccag gtatgcgtgt tacgactcgg ggtctgcgcc ggtggattgt
     1381 tggaacggcg ggcctgctgc tgctggtcgt cgccggcttc gtcatctacg ggcgcaatcg
     1441 gttccggcac atcgaaaaag acctgccggg acgcctgggg atcagcatac agtccacctc
     1501 cagcaccatc acgttctccc aatccaccca ggggcacacg ctctttacgc tgaaagcggc
     1561 gaagcaggtg cagatgaagt ccgggcatgt gctgctgcac gatgtcgaca tcactttgta
     1621 cggaccgcca ggctcgggac gcaaggatcg catcgccggc acggatttcg attacgacca
     1681 gacccacggg atcatcatca gccagggaga cgtggagatc gaacttcagg gggccggcaa
     1741 aaatgtagcg ccgagcgggg aagacggcga tgcgaacgca aacaccatgc gcgtgaagac
     1801 cagcggcctg accttcctgc agaagacggg cgcggcttct acgcagcagc aggtggagtt
     1861 tgagctgccg cgcgccgccg gaaccgcagt cggcgccgac tacaacgcga agaccggcgt
     1921 cgtggttctc gatcatgacg tgcatatcac gagcagcacc aacggcaaag ctgcggtggt
     1981 cgatgcaacg catgcgacac tgctgcgcga atccaaccag gcccagctgg tgaacgcgag
     2041 agtgagctat cagaatgagg aagccggctc cgatcaggcg accgtgaatt ttcgccggga
     2101 tggaacggcg gaagaaatcg atgcccaggg ccacgttcgc atgcggaccg acgacggtgc
     2161 aacggtcaac gcggaaaccg cgcacattct gctcgacaca aagagcgagc cgacgcgagc
     2221 tgatcttgcc ggcggcgtac agtactcatc ggcccgaaac aacgcgacga tgcacggatc
     2281 ctccggtagt ggaacgctgc tctttgccgt gatcaacggc gcggggggcg cgcgcaccag
     2341 tctgcgccac gctgaatttc gcgaagatgt gcggttcatc caaaatgtca cgggtcttgc
     2401 gaaggatctg cgcgggcacg ctcagaaaga aatgcaggcg cagcaactta atgtggagtt
     2461 cgcgcccgcc cgcaaggggc gtggcttcga ggcgcggaag gccatcgcgg agggcagccc
     2521 ggtgatcacg tcgcgggaga
//
LOCUS       DASZYN010000519         8306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1050395, whole genome shotgun sequence.
ACCESSION   DASZYN010000519 DASZYN010000000
VERSION     DASZYN010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8306
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050395"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..985)
                     /locus_tag="VHE33_13305"
     CDS             complement(203..985)
                     /locus_tag="VHE33_13305"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: protein motif:HMM:NF012571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HVT98474.1"
                     /translation="MNPPGVAGADAQKRADGKMTVAEPYLIQQKWDAYTGEQHAVWAE
                     LVRRRMPQLREHAAEEYLNGFEQIGLKEDTIPNLAEVNKRLSPRTGWNATPVSGFLPP
                     DAFFEMLGARQFPTTTYIRSRESMEYTPEPDIFHDVFGHVPMHAHPVFADFLQHYGRV
                     CAGLMDDKEKLEKMGRLFWFTVEFGVIRQKGKVKLYGSGLISSHGEGTHVIEGRPEIR
                     DFNLDQVLNQQFLVSEEQKVLYAVESFDEIYEAAQEAEKRLG"
     gene            complement(1088..2380)
                     /locus_tag="VHE33_13310"
     CDS             complement(1088..2380)
                     /locus_tag="VHE33_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98475.1"
                     /translation="MLKVREARGRWPVCTALAALLAALALCPAAAMPEKKKPASPPPF
                     PEAKPAVTIDVGPLGFRPPGAFYLTYRLSSAAIGFFDDDHLLFTFHMGGLLKRSPDDP
                     RGDDDQEIRAVVLDLGTGKVTQQAKWRLHDRSQYLWPYKDGKFLVRVRDSLFMTDQGL
                     QLEPYMTFDRGIRAMQVSPDHRLLVVETNDPAKLSDQSPDQKFLGGPPVKVRIYALGE
                     KRLIAESSAKVPTLLPLVGDGLLDLLEGRAQKSWVMRDVPFEGEPRTVAEVKSSCQPS
                     ARPLSATVVLTVGCYGEGADDRQVIAVSTKGDGELWRDRWQNKFVWGWFDSAANGSRF
                     IYESIQVNRSISTFDSLYPEDIQAQMVGVYDTETGKLVLVRDASPVLTAGQNAALSPD
                     GKRFAILRNGAIEVYDLPPVSKPEPKKAPEMVAKKPGT"
     gene            2657..3124
                     /locus_tag="VHE33_13315"
     CDS             2657..3124
                     /locus_tag="VHE33_13315"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT98476.1"
                     /translation="MLQDYQNALQFMQQGKYEKARVLFEKLSRDASPEILERCRVYLS
                     VCERNVQEASLTFKNTDEQYDYAVSLLNSGSYEEARDQFESILTAAPDSDYAHYGMAL
                     LSSMTGQAEEALEHLSRAIQINAANRIQARSDPDFIDMADDPRFTELLYPESS"
     gene            complement(3347..3667)
                     /locus_tag="VHE33_13320"
     CDS             complement(3347..3667)
                     /locus_tag="VHE33_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98477.1"
                     /translation="MRTVLTSWKEIAQYMGKGVRSVQRWEKEFGLPVRRPSSGSHHAV
                     FAIAEEIDAWARGQARAERSELERLRVEVAELRKENATLRAQLREADPSAPDIQISDY
                     FSAA"
     gene            complement(3691..4095)
                     /locus_tag="VHE33_13325"
     CDS             complement(3691..4095)
                     /locus_tag="VHE33_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT98478.1"
                     /translation="MDLSVILNVGSDATLLDTRRMILQAAGYIVESANSIDEAIERFR
                     LGDFDLVLLCHSLSERDRRRLISLIRASGSGTPVVAVGYGPGQYSDGLADATLDSGPR
                     DLISGVRNVLLRAAETSRLHVIPTGAIPAGDK"
     gene            4473..5174
                     /locus_tag="VHE33_13330"
     CDS             4473..5174
                     /locus_tag="VHE33_13330"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HVT98479.1"
                     /translation="MVRPVYRNRILASLPKPDLERLAPHLSPLTFEKGQSLHGEGQLV
                     DTIYFPEVGVCSIVVNLKDGRIVEVGIIGRDGFVGMAGVVGTGRSSHHAFIQLPGSGF
                     SVAAKTLREHYEASSDLRLCIQRGVQAFFAQSAQTAACNRVHELEERLARWLLMCRDR
                     MDSDKLPLTHEFLAGMLGTRRSTVSVAAGMLQKAGLIEYSRGIVNIENRKGLESATCE
                     CYGVVHSEYVRLGLL"
     gene            5368..6411
                     /locus_tag="VHE33_13335"
     CDS             5368..6411
                     /locus_tag="VHE33_13335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HVT98480.1"
                     /translation="MSLKTVNPTSSTGLRVVAIGGGTGLSTLLRGLKLRVPHAGLLAQ
                     PATQPLIADLTAVVTVTDDGGSSGRLRRDFNMLPPGDLRNCMVALSEDEGLISRLFRH
                     RFDKGDLEGHNFGNLFIAALTEMTGDFAQAIRLSAEILATRGHIFPATASNVTVCARM
                     EDGSIVRGETNITASRQRIVELMLDPPDPPPPPETLAAIAAADLITIGPGSLFTSIIT
                     NLLVKGISEALAAAQGTRVYICNLMTQANESLHLTASQHIERIYEHAGGAIFDYALVN
                     TAPVSDEIRARYAAEGAEITIADVDRIQSLGIQCITGNFAAEGDVLRHASDRVSTALL
                     SLPVRTSAMAGAK"
     gene            6693..7979
                     /locus_tag="VHE33_13340"
     CDS             6693..7979
                     /locus_tag="VHE33_13340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVT98481.1"
                     /translation="MAQYRMLIECARPGDPRRSNQERESRLSAGSLAACRRRRLPCPA
                     PLFRALPGLTLLFGLLTPASFAIDLSDATRPNAHVHPISAARTTGIHAAHSEFQTPHL
                     WPMIQQIRFNHEALSFDQEITVGPDEGDLEIQFAPAASADHLRYRLIGFDTEWKEAGK
                     PAEVLYSRLAPGRYEFDFQMAESTGLKGSLVQGIPITVIAPWWETARFRGLCIISLLF
                     VILVLYRVHVSWLVKHNRKLQESVTQTRAELTLTAKMAGDAQEALKEQALKDSLTGLW
                     NRRAIFAMLEREVFRAQRDHFPITLVMIDLDHFKNINDTHGHLTGDEVLRETAARLTD
                     AMRPYDFAGRYGGEEFLVVLPSCSPQNGLQRAEHFRRAIADKLLPTAMGALAVTCSLG
                     VAAYEDGMPPEDLIHRADEALYRAKRLGRNCVCAGN"
     gene            complement(8088..>8306)
                     /locus_tag="VHE33_13345"
     CDS             complement(8088..>8306)
                     /locus_tag="VHE33_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98482.1"
                     /translation="RQTTMSIRQSDGTFDGKTIVLWDTPLDTEHQGRKTVARAHLEDG
                     GHVLVHQHFVIDDQGKERMMIELRMTRK"
BASE COUNT         1568 a         2916 c         2183 g         1639 t
ORIGIN      
        1 aacgggccgc acttccgccg gtgaatccca cctccgcagc agccaccgaa ccgagggggt
       61 catgcctggg agagcgttca gcgttcagtc ttcagcgctc agaaaaacgc cgaacgccgc
      121 ttttcctgaa cgccgaacgc cgatcgccga acgcggagcg tcgcttttcc tgaacgctga
      181 gcgctgacaa ctgaacgctg cgtcacccca gcctcttctc cgcctcctgc gccgcctcgt
      241 aaatctcgtc gaacgactcc accgcgtaca gcaccttctg ctcctccgac acaaggaact
      301 gctggttcag cacctgatcc agattgaagt cgcgaatctc cggacggccc tcgatcacgt
      361 gcgtgccttc tccgtgagag gagatcagcc cgctgccgta gagcttcacc ttgcccttct
      421 gccggatcac cccaaactcc accgtgaacc agaagaggcg gcccatcttc tcgagctttt
      481 ctttgtcgtc catcagcccg gcgcacaccc gtccgtaatg ctgcagaaaa tcggcaaaca
      541 ccggatgcgc gtgcatcggc acatgcccaa acacatcgtg aaagatgtcc ggctccggcg
      601 tgtactccat cgactcgcgg ctccggatgt acgtcgtcgt gggaaactgc cgcgccccga
      661 gcatctcgaa gaaggcatcc ggaggcagaa atccgctcac cggcgtcgca ttccaaccgg
      721 tcctcggcga caggcgcttg ttcacctcgg ccaggttcgg aatcgtgtct tccttcagtc
      781 caatctgctc aaacccgttc agatactctt cggccgcgtg ctcccgcaac tggggcatgc
      841 ggcgccgcac cagctccgcc cacaccgcgt gctgttcccc ggtgtatgca tcccacttct
      901 gctggatcag gtaaggctcg gcgaccgtca tctttccgtc cgcgcgcttc tgcgcgtccg
      961 cacccgcaac ccctggtgga ttcattcgga acacccccgg caaaacgtta gagaatttca
     1021 cccggctcgc agggatgtca atggcaatcg agctttgcgt ccgtcaggtt gactcccgcc
     1081 tgtctaccta cgtccccggc ttcttcgcca ccatctcggg cgctttcttc ggctccggct
     1141 tactcaccgg cggcaaatcg taaacctcga tcgctccgtt ccggagaatc gcaaaccgct
     1201 tcccatccgg cgacaatgcc gcattctgcc ccgccgtcag cacgggcgac gcatcccgca
     1261 ccagcaccag cttgcccgtc tccgtgtcgt acacccccac catctgcgcc tgaatatctt
     1321 ccggatagag cgagtcgaat gtcgaaatcg accggttgac ctggatggac tcatagatga
     1381 aacggctccc gttcgccgcc gaatcgaacc atccccacac aaacttgttc tgccagcggt
     1441 cccgccataa ctccccatcg cccttcgtcg ataccgcaat cacctgccgg tcgtcggcgc
     1501 cctcgccgta acaccccacc gtgagcacca cggtcgcgct cagcggccgc gccgagggct
     1561 ggcagctcga tttcacctcg gccaccgtgc gcggttcacc ctcgaacggc acatcccgca
     1621 tcacccacga cttctgcgcc ctcccttcca gcaggtccag caacccatcg cccaccagtg
     1681 gcagcagcgt cggtactttg gcactggatt ccgcgatcag cctcttctcc cccagtgcat
     1741 agatccgaac cttcaccggc ggcccgccca gaaacttctg gtccggagac tgatcgctca
     1801 gcttcgccgg atcgttcgtc tcgaccacca gcagccggtg gtccggcgaa acctgcatcg
     1861 cccgtatccc cctgtcgaat gtcatgtagg gctccagctg caggccctgg tctgtcatga
     1921 acagcgaatc gcgcacccgg accaggaatt tcccatcctt atagggccac aggtactgcg
     1981 agcgatcgtg caatcgccac tttgcctgtt gggtgacctt tccggttccc agatccagca
     2041 ccacggctcg aatctcctgg tcgtcatccc cccgcggatc gtcgggagat cgtttcaaca
     2101 acccgcccat gtgaaaggta aacagcaggt ggtcgtcatc gaagaacccg atcgccgccg
     2161 acgacaaccg ataagtcaga tagaacgcgc ccggcgggcg gaaccccagc ggacccacgt
     2221 cgatggtcac ggccggtttt gcctcaggaa acgggggcgg cgacgccggc ttcttctttt
     2281 ccggcatggc cgcggccggg caaagagcca gcgccgccag cagcgcggcc agcgccgtac
     2341 aaaccggcca tctccccctt gcctcacgca ccttcagcat tccaattcca ttcttcgcgc
     2401 cctgaccggt acagggcaaa cgccaacata caccaggcaa aattccgccg tcccctttac
     2461 tatttgacgg gacgccgcat tgtgcgtata aaacggtttc tacaggttgc acggaagacg
     2521 atgctgcccc cggctgcgtc ttcgaggcag atcgcttatg gcccgaatgc aggaagctcg
     2581 cgaaacatcc acggctcgcc gctccccccg ctccaatccg tcaaaatcca ccgcaccgac
     2641 gagcgaaaat aaacagatgc ttcaggatta ccagaacgcc ctccagttca tgcagcaggg
     2701 caagtacgag aaagcccgcg tcctcttcga gaagctctcc cgcgatgcct ctccggaaat
     2761 cctcgagcgc tgccgcgtct atctcagcgt ctgcgagcgt aacgtccagg aagcctccct
     2821 caccttcaaa aataccgacg agcagtatga ttacgcggtt tcgctcctga actccggctc
     2881 ctacgaagag gcccgcgacc agttcgagtc catcctcacc gcggcgcccg actccgatta
     2941 cgcccactac ggcatggctt tgctcagcag catgaccggc caggcggaag aggccctcga
     3001 acacctgagc cgcgcgatcc agatcaatgc cgcgaaccgc attcaggccc gctccgatcc
     3061 cgacttcatc gacatggccg acgacccccg cttcaccgag ctcctgtatc cggaaagctc
     3121 ctgacctccg ggcctgaagg tccggctttc aaagaacacg cttcctcccc tgccgaaagg
     3181 cgggcctgct tccacagcaa ggaggcaaag gcttccgctt cccgaatttc aggaggcgcg
     3241 gttccttcga aattcaacgt tgactagccg gcgcgcgcgt tcgtcaagat cgcgcgttgc
     3301 atgctcggat cccactgcgg ggtggccggc gatccgggca acagcctcag gcggcggaaa
     3361 agtagtcgga gatttggata tcgggcgcgg agggatcggc ctccctgagt tgggcccgca
     3421 gcgtggcgtt ctctttgcgg agctcggcca cttcaacccg aagccgctcc agctccgatc
     3481 gttcggctcg ggcctgcccg cgcgcccagg cgtcgatctc ctcggcaatg gcaaacaccg
     3541 cgtgatgaga gccgctcgat ggccgccgta ccggcaagcc gaattctttc tcccatcgct
     3601 gcacgctgcg aacccccttg cccatgtatt gagcaatttc cttccacgag gtcagcacgg
     3661 ttcgcattga aagaactcct ctccctggat ttatttgtcc ccggctggaa tcgcaccggt
     3721 cgggatcacg tgcagtcgac tcgtttcggc agcccgcagc agtacgttcc tcacaccgct
     3781 gatcagatcc ctcgggccgc tgtccagggt tgcgtcggcg aggccatcgg agtactgacc
     3841 gggcccatag ccaaccgcaa ccacgggtgt ccctgagcct gaagcccgaa tcaggctaat
     3901 cagacgcctt ctgtccctct cggatagcga gtgacaaagc aggacgagat cgaaatcgcc
     3961 caggcgaaat cgttcaatcg cctcgtcgat ggaattggcc gactccacga tgtagccggc
     4021 ggcttgcaga atcatccgtc gtgtgtcgag aagggttgca tccgagccaa cattcagaat
     4081 gacggacaag tccatgatgc tttcccctta cgttctgacg atgatgcaca tcctctctgg
     4141 cttccggcgc cgcccctctt aacaggcagg atcgtttccc aggccgtgct caacctcacc
     4201 ggatacccaa attgtccacc catggctgcc atctgtgtct gtcgtttacc ggacatagcc
     4261 tcaatatttc tctaatattt ttttgttagc tatgtcaaac aacagttggg atttggccat
     4321 cctcgcaccg tctgcgcagc gtacgattgt gacagacatc ttgattcgca acccagcctg
     4381 aaatttccgg tgaattaggt ttccttgcgc gtttgggtgt gtggaccctt tggcccaccg
     4441 cactgcgaag gagcccgccg atgaaggctg gaatggttcg acctgtctat agaaatcgta
     4501 tccttgcctc cctccccaaa ccggatctcg aacgcctcgc ccctcacctc tcgcccctca
     4561 ctttcgagaa aggtcaaagc ctgcacggcg agggacaact cgtcgacacc atttatttcc
     4621 ccgaagttgg cgtttgttcg atcgtcgtga acctgaagga tggccgcatt gttgaagtcg
     4681 gcatcattgg ccgcgacggc ttcgtcggca tggccggcgt cgtggggacg gggcgatcaa
     4741 gccatcacgc cttcattcag ttaccagggt ccggcttcag cgtcgcagcc aagaccctgc
     4801 gcgagcatta cgaggcctcc agcgatcttc gcctttgcat ccagcgcggc gttcaggcat
     4861 tctttgcgca atcggcccaa accgcggcct gcaaccgcgt ccacgaactg gaggagcgtc
     4921 tggcccgctg gctcctcatg tgtcgcgaca gaatggattc ggacaagctg cccctcaccc
     4981 acgaatttct cgccggaatg ctgggtaccc gccgctctac cgtgtcggtt gccgcaggca
     5041 tgctgcagaa ggccggcttg attgaatatt cgcggggaat cgtgaacatc gagaaccgca
     5101 aggggcttga gtccgccacc tgcgaatgtt atggcgtcgt ccacagcgag tacgtgcgcc
     5161 tggggcttct ctgagttcgc gtccggctcc atcgatcgcg gtcgagcgcg gcttcgggct
     5221 tgaaccgcca ggttgatgct cgaggtgggc tcgcatatcg cagccctcca caatccgacg
     5281 gcgtccactt ttctgccggc cacttgatag ccgactccct ttccagttgg cctgcgttag
     5341 actcataccc gtaagcccaa tcaaccggtg agcctcaaaa ccgtgaatcc aacttcctcc
     5401 acggggctgc gcgttgtagc catcggtgga ggaaccggcc tttcgaccct cctgcgtgga
     5461 ctcaagctcc gcgtcccgca tgccggcctc cttgcccagc cggccacgca acccctgatc
     5521 gccgatctca ctgctgtcgt taccgtcacc gacgatggtg gttccagcgg ccgtctccgc
     5581 cgcgatttca atatgctgcc ccccggcgac ctccgcaact gcatggtcgc gctctccgaa
     5641 gacgaaggcc tcatctcccg cctgttccgc caccgcttcg ataaaggaga cctggagggc
     5701 cacaacttcg gcaacctgtt catcgcggcc ctcaccgaga tgaccggcga cttcgcccag
     5761 gccatccgtc tttccgccga aatcctcgcc acgcgcggcc atattttccc cgccactgcc
     5821 tcgaacgtca ccgtgtgcgc gcgcatggaa gacggatcca tcgtgcgcgg cgaaaccaac
     5881 atcaccgcga gccgtcagcg catcgtcgag ctcatgctcg atccgcccga cccgccgcct
     5941 cctccggaaa cgctcgccgc catcgccgcc gccgacctta tcaccatcgg ccccgggtcg
     6001 ttattcacgt ccatcattac caatctgctg gtcaaaggga tctccgaagc tcttgccgca
     6061 gcccagggca cgcgcgttta catctgcaac cttatgaccc aggcgaatga gagcctccac
     6121 ctcaccgcct cgcagcacat cgagcggatc tacgagcatg ccggcggcgc catcttcgac
     6181 tacgccctcg tcaacacagc gcccgtgtcg gacgaaatcc gcgcgcgcta cgccgccgaa
     6241 ggcgccgaga tcaccattgc cgacgtcgac cgcatccagt ccctcggtat ccagtgcatc
     6301 accggcaatt ttgccgccga aggagacgtg ctccggcatg cctccgaccg cgtcagcact
     6361 gcgcttctgt ccctccccgt tcgcacatct gccatggcgg gcgctaagta gcgggcgcgg
     6421 agcgccgaac gcgattctgc acgctgaacg ctgacaactg aacgctgtcg tgaccctttc
     6481 gtcatgcccc caaactcttt ggtgcttcaa tttaggactg taggtatgtg cttaagtggt
     6541 atagcgaagc gcctttcgat cgtcgatcat ctctcctgac cacccgaccg gataagaagt
     6601 tgggggtctg gtgactccgg tcaccgctca actgccttga agcagattta cctgagtcgc
     6661 atgacaggca aatcagggga agcgccacca aaatggcgca atatcgaatg ctcattgaat
     6721 gcgcgcgtcc cggcgatcca cgccggtcca atcaggaacg agagtcacgc ctgtccgccg
     6781 gatcgctcgc ggcgtgccga cgccgacggc tgccgtgccc cgctcctctt tttcgcgccc
     6841 ttcccgggtt aaccctgctg ttcggccttc tcacacccgc ctctttcgcg atcgaccttt
     6901 ccgatgcgac caggccgaac gcccatgtcc atccgatcag tgcggctcgc accaccggca
     6961 tccacgctgc gcacagcgaa ttccaaacgc cccatctctg gccgatgatc cagcagatcc
     7021 gcttcaatca tgaagcgctc agcttcgacc aggaaatcac cgtcggtccg gacgagggcg
     7081 acctcgagat tcagtttgcc cctgccgcct ccgccgacca tctccgttac cgcctcattg
     7141 gtttcgatac cgagtggaag gaagccggca aaccagccga ggtcctctac agccgtcttg
     7201 cgccgggccg ttacgagttc gatttccaga tggccgagag caccggcctc aaaggctccc
     7261 tcgtccaggg catcccgatt acggtcatcg cgccctggtg ggagaccgcg cgtttccgcg
     7321 gcctttgcat tatcagcctg ctgttcgtga tcttggttct ctacagggtg cacgtgtcct
     7381 ggctcgtgaa acacaaccga aagctccagg agagcgtcac gcaaacccgg gctgagttaa
     7441 ctctgaccgc taaaatggcc ggcgatgctc aggaggccct gaaggaacag gcgctcaaag
     7501 acagcctgac cggcctgtgg aatcgacgag ccatcttcgc catgctggag cgggaagtct
     7561 tccgtgccca gcgggatcac ttccccatca ccctggtcat gatcgatctc gaccacttca
     7621 agaacatcaa cgatacccac ggccatctca ccggtgatga ggtcctccgc gagaccgcgg
     7681 cccgtctcac cgatgccatg cgcccctatg actttgccgg ccgctatggc ggcgaggaat
     7741 ttctggtcgt gctgcccagt tgttcgcccc agaacgggct gcagcgcgcg gaacactttc
     7801 gtcgcgccat cgccgacaag ctgctcccca ccgccatggg agcgctggcc gtcacctgca
     7861 gcctgggcgt agccgcctac gaggacggga tgcctcccga agacctcatc catcgcgccg
     7921 acgaagcgct ttaccgcgcc aaacggctcg gccgcaactg cgtctgcgcg ggcaattagt
     7981 gtggcgtttc ttcaccttgt catcccgacc ggaccctgcg catagcgaag gggaagcgga
     8041 gggacctgct tctcttccat aaagaggttt caaacaccac atcagagcta cttccgggtc
     8101 atcctcagtt cgatcatcat ccgctctttt ccctggtcat cgatcacgaa atgctggtgc
     8161 accagcacgt gcccgccgtc ctccagatgc gcgcgcgcca cggtcttccg cccctgatgt
     8221 tccgtatcga gcggtgtgtc ccacagcacg atggtcttcc cgtcgaaggt cccgtcgctc
     8281 tgccggatcg acatcgtcgt ctgccg
//
LOCUS       DASZYN010000520         4313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_154214, whole genome shotgun sequence.
ACCESSION   DASZYN010000520 DASZYN010000000
VERSION     DASZYN010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154214"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1217)
                     /locus_tag="VHE33_13350"
     CDS             complement(135..1217)
                     /locus_tag="VHE33_13350"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HVT98483.1"
                     /translation="MREAPRPDSEDRTNWKTAALFLLTLAVLSVCGLILYPFLAGITG
                     AVVLAVVTRQPHHWWRKKIRSRTAAAVTSLLLVTISIIAPVLLLVDYLARQVASGAAK
                     LEDGWVQRSLDALLERFPQIATAIEHSSEFMTLGEAAQKVIGYATSHLGAVLSNSLGA
                     VSQVVIMLFLLFFLYRDEEVAVGFLRRLMPLTESETRLLVGRIGETIRATVLGRLVVA
                     LVQGTVAGTVFALLGVHGAVILGLLTATVGLVPPFGAYIVWLPVAAWFAINGHWVKMA
                     ILLAVGSLIISTLDNFLYPALVGTRLRQHTAAVFLSLLGGVWLFGLPGLVLGPLLFSA
                     AEALLSIWRARMGEAGSETPFHSAGA"
     gene            complement(1361..2494)
                     /locus_tag="VHE33_13355"
     CDS             complement(1361..2494)
                     /locus_tag="VHE33_13355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT98484.1"
                     /translation="MISSSNAATAWWQSVRSSQQLDGFTIAGKKATAWLSEETEDRGW
                     DQFLQENPAGQFQQSTIWAQAKAAENWSCARVLIGGEDRLMGGFQLLWRASRFGRIGY
                     VSKGPVLEPLEARADTEILERYAVVLLKDVARRKKLRAVIVQPPDFCEAMSGSLRNAG
                     LLPGGRMGVNDATWMVDVSDGFAAVERRMDRAFRKTLDRAERGNLRVREGGRADLPGF
                     WELMLASCRRQGVEPNPSELRHLLALWDAAHPRGAVRLHVVEKDGEPIAEQLDLLFGK
                     TVTLWKKGWSGAEKRLSPNDVCVYECIRWASEAGYSWCDFTSFDRKMAEAMLAGQTLT
                     EEQKQSRYVNFFRTGGRPVLLPKAQVWVPNRILRAGYRWRYSG"
     gene            2686..3519
                     /locus_tag="VHE33_13360"
     CDS             2686..3519
                     /locus_tag="VHE33_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98485.1"
                     /translation="MSPVTRFIFLLLCAIGTPAVVHAQMPFYTDNADVTDPGTLHFES
                     YDEFDGLQSAQYPDLRQNTFNYKFNYGLPHGFEIDADAPWLTINRAPGSSDANGPGDT
                     EIGIKWNFRRSVRPLSAPALSTSFYVEFPTGDKQQQLSSGLSDYWLNFIAQEPLSDKI
                     RINANFGFLFSGNTSTGVIGTENTRGQVYTGGLSLQRDFTPHLILGAELYGAVADNKS
                     LGKDQLQALTGGSYQFMRRYAVTFAVLGGSHIASPKIGGQIGFEADFPLRHSPRSAND
                     P"
     gene            3718..>4313
                     /locus_tag="VHE33_13365"
     CDS             3718..>4313
                     /locus_tag="VHE33_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98486.1"
                     /translation="MPTRHFPVRPNLDQLRNQAKDLLRAFRRNEPWAVAEFQEALPEK
                     ADSSTAKLADAQFVLARTYGLPHWPRLVIACRITDAIWRGDVETVRSLVLHDPRLLEE
                     DARGVKGNWGPPLSYAANLGQDAVIDMLRSLGASDLPFAFQRACLQGQIQTARRLHSM
                     MGNTRPSGGELSGPAYTLNVAGTELLLDLGAPLYDKNGA"
BASE COUNT          901 a         1584 c         1067 g          761 t
ORIGIN      
        1 accgtagaag ccgtccgcat gggcaaaaag aagatgttcg cgtcgtccgc caaagccgaa
       61 cgcgagttag gcttccggat ggttcccgtc tacgaagcct tacgaaaagc catcgactgg
      121 ttccggcaac gtggctacgc ccccgcggaa tgaaagggcg tctccgatcc agcttctccc
      181 atcctcgccc gccagatcga caacagagcc tcagccgccg aaaacaagag gggccccagc
      241 accagacccg gcagcccaaa cagccataca ccacccagaa gggacaaaaa caccgccgca
      301 gtgtgctggc gcaaccgcgt gcccaccagc gccggataaa gaaagttgtc cagcgtactg
      361 ataatcagcg agcccaccgc gagcagaatc gccatcttca cccagtgacc gttgatcgca
      421 aaccacgcgg caacgggcag ccacacaatg taggcaccaa atggaggcac caggccgacg
      481 gtcgccgtca gcagaccaag aatcaccgcc ccatgtaccc ccagcagcgc aaaaacggtg
      541 cccgccaccg tgccctgcac cagcgccacc accagccggc ccagcaccgt cgctcgtatc
      601 gtctctccga tccgcccaac caaaagcctt gtctcgctct ccgtgagcgg catcagccga
      661 cgcagaaagc cgaccgccac ctcttcatcg cgataaagaa agaagaggag gaacagcatg
      721 atcaccacct gactcaccgc acccagagaa ttactgagca cagcccccag atgcgacgtt
      781 gcgtatccga ttaccttttg tgcggcctcg cccagcgtca tgaattccga gctgtgctcg
      841 atggccgtcg caatctgcgg gaaccgctcc agcaaggcat caaggctgcg ctgcacccat
      901 ccgtcttcca gcttcgccgc cccgctcgcc acctgcctgg ccagataatc caccagcaga
      961 aggaccgggg caatgatgct gatcgtcacc agcagcagcg aggtcaccgc cgccgccgtg
     1021 cgactccgga tcttcttccg ccaccagtga tgcggctggc gcgttactac cgccagaacc
     1081 acggcccccg tgatgcccgc gagaaagggg tacagaatca gaccgcaaac actcagcacg
     1141 gccagcgtga gcagaaacaa agccgccgtc ttccagttgg ttcgatcctc cgaatccggc
     1201 cttggcgctt ccctcatgct ggccaccctc cgctccaggc tgacgcccaa ctccgtgccg
     1261 gcctgaacgc gacaggatcg taccagacac gccggcggtc atggtgagtc ctcgcccgtc
     1321 cgggttcagc tccgcctaat cagcccgggt tccccgttca ctacccggaa taccgccacc
     1381 gataccccgc ccgcaaaatc cggttcggca cccacacctg cgccttcggc agcaatactg
     1441 gccttccccc cgtccgaaag aaattcacgt accggctctg cttctgctct tcggtcagcg
     1501 tctgccccgc cagcattgcc tccgccatct tccgatcgaa gctcgtgaaa tcgcaccacg
     1561 aatacccggc ctcgctcgcc cagcgaatgc actcgtacac gcaaacgtca ttcggtgaca
     1621 gccgcttctc cgcgccgctc cagcccttct tccataaggt cactgtcttg ccgaaaagaa
     1681 gatcgagttg ttccgcaatc ggctcgccat ccttctccac gacatgcaag cgaaccgctc
     1741 ccctcggatg cgccgcatcc cacagcgcca gcaaatgccg cagctccgaa ggattcggct
     1801 ccaccccctg ccgccggcac gaagccagca tcagttccca aaacccaggc agatccgctc
     1861 gccctccctc tcgcacgcgc agattcccgc gctcggcgcg gtccagcgtt ttgcggaagg
     1921 ctcggtccat acgccgctcc accgctgcaa acccgtctga cacatccacc atccaggtcg
     1981 catcgttgac ccccatgcgt ccacccggga gcagaccagc gtttcggagg gaaccgctca
     2041 tcgcttcgca gaaatccggt ggctgaacga tgaccgcacg cagtttcttc ctccgtgcca
     2101 cgtccttcag caacacgact gcataccgct ccagaatctc cgtatcggct cgagcctcca
     2161 gcggctccaa aaccggtccc ttgctgacgt aaccaatcct gccaaaccgc gaagcccgcc
     2221 agagcaactg gaatcccccc atcagcctgt cttccccgcc aatcagcaca cgagcgcagg
     2281 accagttttc agcggctttc gcctgggccc atatcgtgga ctgctggaac tgaccagccg
     2341 gattttcctg gaggaattga tcccaccccc ggtcctctgt ttcttcactc aaccatgctg
     2401 tcgccttttt cccggcgatt gtgaatccgt ccagctgctg ggaggaccga accgactgcc
     2461 accacgccgt tgcggcatta gaggatgaga tcaccggcaa gaagtcccct ccgtttcgag
     2521 tttccggaac aggtcacaag ccggcatcaa ccgatattag ccaaccggcc ctcacacaca
     2581 aatcaccaaa acggatcacc agccgcgctt tggacaaccg gcgctgtttt catcctgcgt
     2641 tgacaccgcg aacaaaacgc gcccatttac agtgatcaaa cggacgtgag ccctgtgacc
     2701 cgattcatct tcctcctgtt gtgcgcgatt ggaacccccg ccgtcgtcca cgcgcaaatg
     2761 cccttctata cggacaatgc cgacgtcact gaccctggca cgctgcactt cgagtcctac
     2821 gacgaattcg atggcctgca atcggctcaa tacccagacc ttcggcagaa caccttcaac
     2881 tacaagttca attatggtct gccgcacggc ttcgagatcg acgcggatgc gccctggctc
     2941 accatcaacc gtgcccccgg ttcctccgac gccaacggcc ccggggatac cgaaattggc
     3001 atcaagtgga acttccgcag atccgtccgt cctctcagcg cgccggccct ctccaccagc
     3061 ttctacgtcg aattccctac cggcgacaaa cagcagcagc tcagctcggg tctttccgat
     3121 tactggctca acttcatcgc ccaggagccg ctctccgaca agattcgcat caacgccaac
     3181 ttcggctttc tcttctccgg caacaccagc accggcgtca tcggaacgga aaacacccgc
     3241 ggccaggtgt acaccggtgg actttcgctc cagcgcgatt tcaccccgca cctcatcctg
     3301 ggcgcggaac tctacggcgc cgtcgccgac aacaagagcc tcggcaaaga ccagctccag
     3361 gctctgaccg gaggctcgta ccagttcatg cgtcgctacg ccgtgacgtt cgccgttctc
     3421 ggcggcagcc acatcgccag cccgaaaatc ggcggccaga tcggatttga agccgacttc
     3481 cccctacgcc actccccccg ctccgcaaac gatccgtaac gtggccccac ggtctcgaaa
     3541 taccctgccc gactttgcgc gaatcctgga cgccgtttac actggcctcg gcgcgcaggg
     3601 cgcagcccgt ccgcggacag gaccgagtcc acctgcaaac atccacgatt catggttcac
     3661 acgaacctcc tttgccccga tagcggatca agggcacgat tctcaagggg gtccgttatg
     3721 cccacccggc actttccggt tcgtcccaat ctcgaccagc taagaaatca ggccaaagac
     3781 ttattgcggg ccttccgccg caacgagccc tgggctgtcg ccgaatttca ggaagctctt
     3841 ccagaaaaag ctgactcttc cacagcgaag ctcgctgacg cgcaattcgt gctcgcgcgc
     3901 acttacggcc ttcctcactg gcctcgcctg gtaatcgctt gccgtatcac cgacgccatc
     3961 tggcgtggcg acgtcgaaac ggttcgctcg ctggtcctcc acgatcctcg tctgctggag
     4021 gaggatgcgc gcggcgtgaa gggaaactgg ggtccgccct tgtcctatgc cgcaaacctc
     4081 ggtcaggatg ccgtcatcga catgctgcgc tcccttggcg cctccgattt gcccttcgca
     4141 ttccagcgag cgtgccttca gggccaaatc caaaccgcgc gccgattgca ctcgatgatg
     4201 gggaataccc ggccttcggg cggcgaacta agcggccccg cctatacgct caatgtcgca
     4261 ggcacggagt tgctgctgga tcttggcgcc cctctctatg acaagaacgg agc
//
LOCUS       DASZYN010000521         6234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1245030, whole genome shotgun sequence.
ACCESSION   DASZYN010000521 DASZYN010000000
VERSION     DASZYN010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245030"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VHE33_13370"
     CDS             <1..421
                     /locus_tag="VHE33_13370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HVT98487.1"
                     /translation="AGQGQESAGAMKFSVATHRKREIVDITEQVESHLRNATGICCLN
                     VLHTTAALTTADLDPGTDLDMLDAFEAMMPKLRYRHPHNPEHVPDHILSSLIGTALTL
                     PVEGGSLVLGTWQRVILVELDGPRKRELVLTVVASRD"
     gene            complement(506..766)
                     /locus_tag="VHE33_13375"
     CDS             complement(506..766)
                     /locus_tag="VHE33_13375"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HVT98488.1"
                     /translation="MADVKITVRPNGPYRVEGPVTLVDHEGKEWDVSGKPAFSLCRCG
                     ASVNKPFCDGTHSKIGFQAAEAAVRAENNPTTPGDPTMPVKI"
     gene            934..2499
                     /locus_tag="VHE33_13380"
     CDS             934..2499
                     /locus_tag="VHE33_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98489.1"
                     /translation="MPTLLTHVHTPATPADPEADRRSLRGWLLPFLGVGSLLASCIVW
                     SLRKPMWGDEVFTWTELHDPSLRHLLGAIARLGGGGMPLFSLTAWPWAHGFGFSDLSL
                     RLYSSVAVCGAFLVLFAALRRRLGARAAFLGTSFGFCASLIVLDQNAEARGYGLYLLL
                     AALATAQVLKVAETESWRPRDLFFLAVTQAGLVLGHPLGLLYAGLLLLALIAVDLSAR
                     QLRFRIYLACVAGWLALIPWIPALRASAAVGRPHGWIGVPSVGDLASSLSFWLFTGLY
                     WQIPHLPSMALVAGWLAGVACVTLIVFASAVGLRDSPRTNRAVFFAGCALLLGPSLFF
                     IVSHLTTPIFLPRYLLPSVAGVCLLGAAAADRFRFGKRSSVALGAVFLALPVATALLA
                     RPVGLNVTRVDELAAGRIVVCDSLKDFLVMTRYTTQPASPRFPLDVEVADTLPGADAD
                     VRLMVNYRREGYFAGHLADTQTLLGEPSFLFLGNSGAAWFRMRIAGDPHFTWKTLVRI
                     DSSRSLIEVDRQP"
     gene            complement(2550..3767)
                     /locus_tag="VHE33_13385"
     CDS             complement(2550..3767)
                     /locus_tag="VHE33_13385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HVT98490.1"
                     /translation="MSAAAPPLHAKRQTDFADAMIIFACVVTFTLTTLFLLVMPMVTR
                     LAGGRDYVVYWSTGFQLIHHADPYDPVVMGQLERFAGYDGKPGSYYMRNPPWALPLAL
                     PLGFVPPRVGALPWSLLLLGILIFCVRILWKMFGKPGTYLEWIGYAFPPALQCVIMGQ
                     TSIFLLLGLVLFLRFHRTHPFLAGAALWFCTLKPHLFLPFGLIVLVWIVATRSYRILV
                     GALAALTFSCALTWFIDPAAFSQYAHWARTSGIADEFIPCISVELRQLVNPAAKWLVF
                     VPSALSGFWALAYYWRHRRNWDWLENGNVLILVSILVAPYCWIYDQSLALPALTYGAW
                     RTSSRTAVAVLAGLCIVLEVQPFWATIGLGSKWFLWPAIAWLVWYLVAARYRSAAIGE
                     AAALAPAANVAHV"
     gene            complement(3863..5071)
                     /locus_tag="VHE33_13390"
     CDS             complement(3863..5071)
                     /locus_tag="VHE33_13390"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HVT98491.1"
                     /translation="MAAPAIITKKTSDFADLAIGLACGFVLIVTALLLAVLVQKGGLP
                     SSCDFASYWATGQQLAHHANPWDPAVMGDLERTAGYTGQGSYYMRNPPWALPLVLPLG
                     YVSARVAVLPWSLLMLGLLLLSVYLLQPVLGLSGWQLRLLGCAFPPAMQCVVVGQTSL
                     FVLLGLVLFLRLHRTRPLWAGAALWLCTLKPHLFLPFGLILLLWILLTRSWRILAGAT
                     AAAALSCLAALSIDAAAFSQYWIWAHSSGISSQPVPCFGVMLRNLIDPGAQWLVFVPA
                     ILASAWALAYFWPRRARWDWIEHGSLLMLVSIVAAPYCFFFDQSLALPAVLYAASRTA
                     SRRILSALAALYLLVEIVPWFHFGPTTFFYSWLWQAPAWLVWYLLARRSARHDSALGV
                     AATTFVPAAG"
     gene            complement(5180..5395)
                     /locus_tag="VHE33_13395"
     CDS             complement(5180..5395)
                     /locus_tag="VHE33_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98492.1"
                     /translation="MAQLDEMLDQDAALPLRADLEQHLRECEHCEITLNTTRKTIQIY
                     RSHELYELPNDLRERLHKAILAKCRKC"
     gene            complement(5410..6057)
                     /locus_tag="VHE33_13400"
     CDS             complement(5410..6057)
                     /locus_tag="VHE33_13400"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HVT98493.1"
                     /translation="MSSPIQTQGTPEVHPDVALVERVRQGDMAAYEKLVRQYDRQVFR
                     IANHIVQNREDAEDVVQDAFLKAYEKLDQFQGNSKFYTWLVRIAVNEALMRLRKRRTG
                     KMVSIDEDVETEEGSMPRDLADWSPDPEAMYGQSEMADILRKTIQGLPPGFRVVFVLR
                     DVEGLSTEETAETLGLSIPAVKSRLLRARLQLRERLSRYFKRGQGKQAQAQVGAK"
BASE COUNT         1188 a         1755 c         2107 g         1184 t
ORIGIN      
        1 agcaggacaa ggacaagaat ccgcaggagc catgaaattc agcgtcgcca cccatcgcaa
       61 gcgcgagatt gttgacatca ccgaacaggt ggagtcgcac ttgcggaatg caaccggcat
      121 ctgctgcctc aacgtactgc atacgacggc ggcgttgacc acagcggatc tggatccggg
      181 aacggatctg gacatgctgg atgccttcga ggcgatgatg ccgaagctga gataccgaca
      241 tccgcacaac ccggagcatg tgccggatca catcctgtcg tcgctgatcg gtactgcgct
      301 gacgctgccg gtggagggtg gatcgctggt tctgggtacc tggcagcgtg tgattctggt
      361 ggaactggat gggccgagga agagggaact ggtgctgacc gtagtggcga gcagggatta
      421 gagcctagag tccagccacc gcatgctctc gatttccggc atcgcagatg tttcgagcta
      481 aactctaaac tctaatccct tacaactaaa tcttcaccgg catggtcggg tcgcccggcg
      541 tggtggggtt gttctccgca cggacggcgg cttcggcggc ctggaagcca atcttcgagt
      601 gggtaccgtc gcagaagggc ttgttcaccg aggcgccgca gcggcagagg gagaaggcgg
      661 gtttgccgct cacatcccat tccttgcctt cgtgatcgac gagggtgacg gggccttcga
      721 cgcggtaggg tccgttgggg cgaaccgtga tcttcacatc agccattgca atctcctgga
      781 gagttcgagt taggtttcct gtccagtcta tccagacggc tcgggtgctc ctaattgtgt
      841 tgccgattcg atgcctttcc cccggatggg ccatggcccg gagcggttgg atcgccggag
      901 aatcgaagct gaggatttga tgcaaagagc gcgatgccca cgcttttgac ccatgtgcat
      961 acaccggcca cacccgccga tccagaagcg gatcgtcgtt cgctgagggg ttggcttctg
     1021 ccttttctcg gtgtcggatc acttctggcg tcgtgcattg tgtggtctct gcgtaagccg
     1081 atgtggggcg atgaggtttt tacgtggacc gaactgcacg acccatcgtt gcggcatctg
     1141 ctcggcgcca ttgcccgcct tggaggcgga gggatgccgc tgttttctct cacggcgtgg
     1201 ccgtgggcgc atgggttcgg tttcagcgat ctctctctgc gtctgtactc gtcggtggct
     1261 gtgtgcgggg cgttccttgt cttgttcgca gcgttgcgcc ggagactggg tgcgcgtgcg
     1321 gctttcctgg gcacgtcgtt tggattttgc gcatccctga tcgtgctcga tcaaaacgcg
     1381 gaagcccgtg gttacggact gtacctgctt cttgcagcgc tggcgacggc ccaggtgctg
     1441 aaagtagcgg agaccgaatc gtggcggccg cgtgaccttt tcttccttgc tgtgacccag
     1501 gcgggactgg tgctggggca tccgctgggg cttttgtacg cggggctgct tctgctcgcg
     1561 ctgattgctg tcgacctcag cgcgcgacag ttgcgttttc gcatatatct ggcctgtgtg
     1621 gccggctggc tggcactgat tccctggatt ccggccctgc gggcttcggc tgcggtgggc
     1681 cgtcctcacg gatggatcgg ggttccgagt gtcggcgatc tggcatccag cttgtctttc
     1741 tggctgttca cgggactcta ctggcagatc ccccatctgc cctcgatggc cctcgtcgcc
     1801 ggctggctcg caggagtcgc gtgcgtgact ctgatcgtct tcgcgtccgc agttggcctg
     1861 cgggacagcc cccgaacgaa tcgtgcggtc ttctttgcgg gatgcgcgct tctgctcgga
     1921 ccgtcgctct tcttcatcgt ttcgcatctg accactccga tttttctccc gcgttatttg
     1981 ctgccgtccg tagcgggtgt ttgcctgctg ggggcagcgg cggcggatcg cttccggttc
     2041 ggcaagcgaa gcagcgttgc cctcggagcg gttttcctgg cgcttccggt tgcaactgca
     2101 ttgttggcgc gtcctgtggg cctcaacgtc acacgggtcg atgagcttgc ggcggggcgg
     2161 atcgttgtct gcgacagcct gaaggatttt ctggtgatga cgcggtacac gacgcagccc
     2221 gcatcgccgc ggttcccgct ggatgtcgag gttgcggaca cgcttcctgg agccgatgcc
     2281 gatgttcgcc tgatggtgaa ttatcggcgc gaaggctatt tcgcggggca tcttgccgat
     2341 acgcagacct tgctcggcga gccctcgttt ctgtttctgg gcaattcagg cgcagcgtgg
     2401 ttccgcatgc gcatcgccgg cgatccgcac ttcacctgga agacgctggt gcgaatcgac
     2461 agcagccgga gcctgatcga agtggacagg cagccttaat catggctggt ctgagcatgg
     2521 tgccgacttc cgagctttgc tgattcgagc tagacgtgag caacgttggc cgccggcgca
     2581 agggccgctg cttctccgat cgcggcgctc cggtagcggg cggcgaccag ataccagacg
     2641 agccatgcga tcgcaggcca gaggaaccat tttgagccga ggccgatcgt ggcccagaag
     2701 ggctgcactt caaggacaat gcagagccct gcaagaaccg ccacggctgt gcgcgaggac
     2761 gtgcgccagg cgccgtacgt cagcgcggga agcgcaaggg actgatcgta gatccagcaa
     2821 taaggggcga caagaatcga gacgaggatg aggacgttgc cgttctcgag ccagtcccag
     2881 ttgcggcggt ggcgccagta gtaagccaaa gcccagaatc cgctaagcgc gctgggtacg
     2941 aagacgagcc atttcgcagc ggggttgacg agctggcgca actcgacgct gatgcaggga
     3001 atgaactcat ctgcaatgcc tgaggtgcgt gcccagtggg cgtattggga gaaggctgca
     3061 ggatcgatga accaggtgag ggcacagctg aatgtgaggg ctgccagcgc tccgacgaga
     3121 atgcggtagc tgcgcgtggc gacgatccag acgagcacga tgaggccgaa gggcagaaag
     3181 agatgcggct tgagcgtgca gaaccagaga gccgcgccgg ccaggaaggg gtgcgtgcga
     3241 tggaagcgca gaaagagcac caggccgagg agcaggaaga tggaggtctg gcccatgatg
     3301 acgcactgca gagccggggg aaaggcatag ccgatccatt cgaggtaggt gcctggtttg
     3361 ccgaacatct tccacaggat gcggacgcag aagatgagaa tgccgagcag gagcagggac
     3421 cagggcagcg ctccgacacg cgggggcacg aatccaagcg gcagggcgag gggcagagcc
     3481 cacggcggat tgcgcatgta gtacgagccg ggcttgccgt cgtatccggc gaagcgttcc
     3541 agttgcccca tcacgacggg gtcgtagggg tccgcgtgat ggatgagctg aaagcccgtc
     3601 gaccagtaaa cgacgtagtc gcggccgccc gccaggcgag tgaccatggg catgaccagc
     3661 aggaacaggg tcgtcagcgt gaaggtgacc acgcaggcga agatgatcat cgcatcggcg
     3721 aagtcggttt gccgttttgc gtggagcggc ggggcggctg cggacatccc ttcaatttta
     3781 gaaacgggtg ctgctgccgg cgattccacc ttccggccat gtgccggtac cgcgttctcg
     3841 ggatgctgcg gcgtgtgcga agtcatcctg cagccggcac gaaagtagtt gccgccacac
     3901 ccagggcaga atcatgccgg gcggaccgac gcgcgagcag ataccagacg agccatgcag
     3961 gcgcctgcca gagccacgag tagaagaaag tcgttggacc gaagtgaaac cagggaacga
     4021 tctcgaccag caggtacagg gcggcgagag cggagagaat ccggcgcgag gcggtgcgtg
     4081 aagcggcgta aagcaccgca ggaagcgcaa ggctctgatc gaaaaagaag cagtaagggg
     4141 cggccacgat cgagacgagc atcagaaggc tgccatgctc gatccagtcc caccgggctc
     4201 gccgcggcca gaaataggcg agggcccacg cggaggcaag aattgccggt acgaagacga
     4261 gccattgcgc gcccggatcg atgaggttgc ggagcatcac gccgaagcag gggaccggtt
     4321 gcgatgagat tccggaggag tgcgcccaaa tccagtattg cgaaaaggcg gcggcgtcaa
     4381 tggagagggc ggcgagacag ctgagtgcgg cggctgccgt tgcgccggca aggatgcgcc
     4441 acgaccgcgt gaggaggatc cagagcagca ggatcaagcc gaaaggaagg aagagatgtg
     4501 gcttgagcgt gcagagccag agagcggcgc cggcccacag gggacgcgtt cgatgcaggc
     4561 ggaggaagag gaccaacccg agaaggacga agagcgaagt ctggccgacg acgacgcatt
     4621 gcatcgcggg aggaaacgca cagccaagaa gacgcagttg ccaccctgag aggccgagta
     4681 cgggctggag aaggtagacg gagagaagca gtaagccgag catcagcagc gaccagggca
     4741 ggacggccac gcgcgcgctg acatatccca ggggcagcac gagcggcaga gcccagggtg
     4801 gattgcgcat gtagtaggaa ccctgtccgg tatagccggc ggttcgctcg aggtcgccca
     4861 tgacggcagg atcccaggga ttggcatgat gcgcgagctg ctggccagtg gcccagtaag
     4921 aggcgaagtc gcaggagctg gggaggccgc ccttctgcac gagcacggcg agaagcagtg
     4981 ccgtaacgat gagcacgaac ccgcacgcga ggccgatcgc caggtcggcg aagtcagagg
     5041 tctttttggt gatgatcgcc ggcgctgcca tcgcgtccat tgtattgacg ctggacgtct
     5101 atgctgaatc cctctttagg aagcaatcga gggagaggga atcagtcaca cactaccgga
     5161 gcacgcccgt ctcgacccgc tagcacttgc gacatttggc caggatggcc ttgtggagcc
     5221 gctcgcgcag atcgttgggc aactcgtaca gttcgtggct gcggtagatc tggatggtct
     5281 tgcgcgtggt gttgagcgtg atctcgcagt gttcgcactc gcgcaggtgt tgctcgagat
     5341 ccgcgcgcag cggaagggcc gcgtcctgat cgagcatttc gtcgagctgg gccaaaaact
     5401 ccgtgcaggt cacttcgcac ccacctgggc ctgagcctgt ttgccctgac cgcgcttgaa
     5461 gtagcggctg aggcgttcgc gcagctgcag gcgggctcgc agcagccgcg atttaaccgc
     5521 tgggatgctg aggcccagag tctcggctgt ttcttcagtc gaaagtcctt ctacgtctct
     5581 cagcacaaag accacccgaa agcccggcgg gaggccctga attgttttgc gcaggatgtc
     5641 cgccatctcc gactgtccat acatcgcctc cggatccgga ctccagtcgg ccaggtcgcg
     5701 cggcatcgag ccttcttcgg tctccacgtc ttcatcgatg gaaaccatct tgcccgttcg
     5761 gcgcttcctg agccgcatga gcgcttcgtt gaccgcgatg cgcaccagcc aggtatagaa
     5821 cttggaatta ccctggaact ggtccagctt ctcgtacgct ttcaggaacg catcctggac
     5881 gacgtcttcg gcgtcttcgc gattctggac gatgtggttg gcgatgcgaa aaacctggcg
     5941 gtcgtactgc cgcaccagct tttcgtatgc cgccatatcg ccctggcgaa cgcgctcgac
     6001 gagcgcaacg tcaggatgaa cttcgggcgt cccctgtgtt tggatgggag aagacatgaa
     6061 tggcttcaga atttggacag ctgcagacaa ccgggccttc ctccatagta aacggcgcag
     6121 ggtgggtcga tcaaagacga cgccagctct cctgtgcgca ctggcgcttg ttcttttgat
     6181 gagcgggtgc cggaagaaga ctccgacttt cgcaggcccc ccggatacgg ttcg
//
LOCUS       DASZYN010000522         2325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1050634, whole genome shotgun sequence.
ACCESSION   DASZYN010000522 DASZYN010000000
VERSION     DASZYN010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050634"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VHE33_13405"
     CDS             complement(<1..1104)
                     /locus_tag="VHE33_13405"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT98494.1"
                     /translation="MFSRKRRYQELSESMREHLTERVDHLVSEGVPRKEAEFQARREF
                     GNATRIEEQSREVWQWPRLDSLSADLKYALRQMKRSPGFAMVVIVTLALGIGANTAVF
                     TLVYGILLRSLPVADPSHLVRIGDRDDCCYWNTVQNEDGDFDLFSYDAYRQMKAAAPE
                     FEQLAAIEAGGETFSVRKGSQPSVALRAEYVSGTYFTTLGVGAFTGRPLSENDDRQGA
                     APVLVVSYRAWQSQFGGDPALVGSTVDVEGHAFTVAGIAPPSFFGDRVAPFPPDAWLT
                     LANEPVMEGANSALLDKDEDWLYPLGRLRAGVARGPLQAKLSAVLRQWLMSRPAYTDH
                     GGAALIPKQHVVLADAGGGIQKLQQQTGKGLRML"
     gene            complement(1133..1468)
                     /locus_tag="VHE33_13410"
     CDS             complement(1133..1468)
                     /locus_tag="VHE33_13410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVT98495.1"
                     /translation="MMGKESRDLFPGALELMILQTLYRKPMHGYALAQHIKAISDELL
                     QIEEGSLYPALQRMLKAGWLDSKRGLSPQGREVRIFRVTAAGAKRLEAERSSFERMFA
                     GITRVLGTA"
     gene            1787..2251
                     /locus_tag="VHE33_13415"
     CDS             1787..2251
                     /locus_tag="VHE33_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98496.1"
                     /translation="MSLAVFTQVHVAISLIGIASGFVVIFAMIASKRMPAMTALFLTT
                     TILTSVTGFFFPYHGVTPGIVIGVLSLVVLAVAVMAWFRGWRRTWVLTAIVAQFFNFF
                     VLIVQSFEKVPALHALAPTGKEGPLKAAQIGALVLFVGLAVWAYRSSRPQAA"
BASE COUNT          396 a          685 c          772 g          472 t
ORIGIN      
        1 gagcatgcgc aggcccttgc cggtctgctg ttgcagcttc tggatgcctc caccggcatc
       61 ggcgaggacc acatgctgtt tcggaatcag cgcggcgccg ccgtggtcgg tatatgcagg
      121 ccgggacatc agccactgcc gcaggaccgc cgataatttt gcctgcaggg ggccgcgcgc
      181 aactcccgcg cgcaagcgtc ccaggggata gagccagtcc tcgtccttgt cgaggagcgc
      241 cgagtttgcc ccttccatca ccggctcgtt ggccagcgtc agccaggcat cgggaggaaa
      301 cggagcgacc cggtccccaa agaaactggg cggcgcgatt cctgcgacgg tgaatgcgtg
      361 gccctctaca tccactgtcg atcccacgag agcggggtcg ccgccgaatt ggctctgcca
      421 tgcgcgatag ctcaccacca gcaccggagc cgcgccctgc cgatcgtcgt tttcgctcag
      481 cggccttcct gtaaacgcgc ccacgcccag ggttgtgaag taagttcccg agacatactc
      541 ggctcgcaat gcgacgctcg gctgtgaccc cttgcgcacg ctgaaggtct cgcctcccgc
      601 ctcgatcgca gcaagctgtt cgaactccgg cgccgcggcc ttcatttgcc ggtaagcgtc
      661 ataggaaaac aggtcgaagt cgccgtcctc attctgaacc gtgttccagt agcagcagtc
      721 gtccctgtcg ccgatgcgga ccagatgact ggggtcggca accggcagcg atcgcagcag
      781 gattccgtag acgagcgtga agaccgcggt attggccccg atgcccagcg ccagggttac
      841 gatcaccacc atcgcgaacc ccggcgatct cttcatttgc cgcagcgcgt acttcaggtc
      901 ggcactcagc gagtcgaggc gcggccactg ccacacttcg cggctctgct cctcgatgcg
      961 ggtcgcgttg ccgaactcgc gacgggcctg gaactcggct tctttgcggg gaacgccctc
     1021 gctgacgagg tgatcgacgc gttccgtcag gtgctcgcgc atcgactccg acagttcctg
     1081 gtaacgccgc ttccgcgaaa acatggctgc tccccttttc tgaccgcgaa cgctatgccg
     1141 tgccgaggac gcgcgtgatg cctgcaaaca tgcgctcgaa gctggagcgc tccgcctcga
     1201 ggcgttttgc gcctgcggct gtgacgcgga aaatgcggac ctcgcggcct tgcggggaga
     1261 ggccgcgttt tgaatcgagc cagccggcct tcagcatgcg ctgcagagcg ggatagagcg
     1321 agccttcctc gatctggagg agctcgtcgg agatggcttt gatgtgttgc gccagcgcgt
     1381 agccatgcat tggcttgcgg taaagggtct ggaggatcat gagctccagg gcgccgggaa
     1441 agaggtcacg agattctttg cccatcattg tttacaaata ctatttcgga aatagtctgt
     1501 ccagcatggt ttgtgacgga tgggcggacg gaacagcagc ccctccgctg cgccccgttg
     1561 cggcgcaacc ccgcttcggt cggaatgaca gactcaaatt ggatttgcgc gtttccgggg
     1621 ctgttatgaa ggcgatgagc tatccgtttc ctgcgggcgc gggtaatttt cgcaaaaggc
     1681 gaaggcaggc gcagcggttg gaagtcagac ggcagcggcg gctttggctg gtagtctgta
     1741 gccaaatgca ggaccttcag aaaggatggg aatgatgatt ctcgggatga gtcttgcagt
     1801 tttcacgcag gtgcatgtgg cgatcagcct gatcgggatc gcgtcgggat ttgtcgtgat
     1861 tttcgcgatg atcgcgtcga agcggatgcc ggcgatgacg gctctgtttc tcacgacgac
     1921 aatcctgacc agtgtgacgg gatttttctt tccgtatcac ggggtcacgc cgggcatcgt
     1981 gattggggtt ctgtcgctgg tggtgctggc ggtagcggtg atggcgtggt ttcgcggatg
     2041 gcggcggacg tgggtgctga cggcgatcgt ggcacagttc ttcaacttct ttgtgctgat
     2101 cgtgcagtcg tttgagaagg taccggctct gcatgcgctg gccccgacgg gcaaggaggg
     2161 gccgctgaag gcggcccaga tcggcgcgct ggtgctgttt gtggggctgg cggtctgggc
     2221 gtacaggagc tcccggcctc aggcggcata gcctatggac cgcagtgcgg tgcgtcacag
     2281 cacgccgggc ggcggatcgg gttaagctga agggtgagcc aggcg
//
LOCUS       DASZYN010000523         3823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_402664, whole genome shotgun sequence.
ACCESSION   DASZYN010000523 DASZYN010000000
VERSION     DASZYN010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402664"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /locus_tag="VHE33_13420"
     CDS             complement(<1..1457)
                     /locus_tag="VHE33_13420"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVT98497.1"
                     /translation="MQNGANALWVDTLSEEPHRIGDRAFVSGFPEVRNGSVVLTLSEI
                     ESTQSDVPLSAEKVDPVQLASGSHAFNLVSVEGRLIMRVGEAAQDQYVIASQGRVFSA
                     FYRHPERGLDLPADPMRTFPLGSRVRVTGICVLDRGDQFRGPAAFHLLLRSSQDVAML
                     AGPSPVSVRNLIILLGVLVILVSGAIAKAFLLERKLRRQGVATASTIDRWRTRVIDGI
                     NSAIPLRETLSQITELLSLQLQAEYCWAEIELEGTFGNCPPISEKSSLEVVEHAIPAR
                     SGTVLGKVCTGFRVHGGKHLVTAEAVENAVRLAALAIETGGKYSDLVRRSEVDPLTGA
                     GNRFAFDRALDLAIEREKESGTKFGLIYIDLDGFKQVNDQFGHQVGDRYLQEIVSRLK
                     NQSRSNDLLARIGGDEFAVLVTNLNSGEELQGIAHRIQSCFEAPLYLDLVEFSASASV
                     GCAVYPDDAVTRGALLECADTRMYQAKRQKRHKSV"
     gene            1518..2345
                     /locus_tag="VHE33_13425"
     CDS             1518..2345
                     /locus_tag="VHE33_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98498.1"
                     /translation="MIFNVKGMKNFVELSRQKLARTASRRRHNTQVGKRRYLQQDSDH
                     LRFAVELSRYGSGDLNRAVQQRPQVRRSSVCNLCLNRTSRHEQIEEYAPRRTVSDQVG
                     CTHNGMHRRPPAVQVSTDQRREIRGCRQWRRTVTEKRHASCQNLGPEARSRDPLNQNA
                     VSGLQPQVRSGLDINGCITILHKKVDISLLVVGDRPLKQHRDRLCRSFIRDRVDGRQR
                     SQRRALLCAQRHSYKQTDTARKGGETETSTNHRVRAFSRARSIVRLRKKAFGVTIAR"
     gene            2679..>3823
                     /locus_tag="VHE33_13430"
     CDS             2679..>3823
                     /locus_tag="VHE33_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HVT98499.1"
                     /translation="MRTPLLRRAIALPLMQLTLALLAIVLPAEAARSGHSSERRAGPE
                     VIAYVFPRDHVIQPDEIAPRKLTRINYAFANIQDGRIVAVSPADAPNLTTLVALKQQN
                     PSLQILISAGGWLWSGNFSDAALTPESRSRFIESAVAFIEEHQLDGLDIDWEYPGQIG
                     AGNRFRPEDKRNYTLLLAELRKRFEREQKKLGRPLLLSIAAGASDDFLAHTEMRQVAR
                     AVDTVNLMAYDYYEPGSEPRTGNHAPLYTDPADPKHISADASVRQFESEGVPARKIVL
                     GVPFYGHVWGNVPPANHGLFQPGSPVPNAFARYRDIVGSMLGQGFTRYWDSSASVPYL
                     YNAQKEQFVSYEDPQSLALKCAYVQSMHLGGVMFWEYDGDASGALLDT"
BASE COUNT          820 a         1181 c         1034 g          788 t
ORIGIN      
        1 cttacgctct tgtgtcgctt ttgccgtttt gcctggtaca tgcgcgtgtc ggcacattcc
       61 agcagagcgc ctcgggtgac tgcatcgtct gggtacacgg cacatccaac gctggcagaa
      121 gctgagaact caaccagatc aagatatagc ggcgcttcga aacaactctg aattcggtgc
      181 gcgataccct gcaactcttc gcccgaattc agattcgtga ccagcacggc aaattcatct
      241 cccccaattc gagccaggag atcgttcgaa cgagattgat ttttcaggcg agaaacgatt
      301 tcctgcagat aacggtcacc cacctggtgg ccgaactgat cgttgacctg cttgaatcca
      361 tccaggtcga tataaatcaa cccaaacttc gtgcctgatt ctttttccct ttcgatcgcc
      421 agatcgagag cccgatcgaa ggcgaagcga tttcctgcgc ctgtcagcgg atcgacttcg
      481 gaccggcgca ccagatcgga gtattttcca cccgtttcaa tggcgagagc agcgagacgt
      541 acggcatttt cgaccgcttc cgcagtgacc aggtgtttcc cgccgtgcac ccggaatccc
      601 gtgcatacct tgccgagaac cgtccccgag cgtgcaggga ttgcgtgttc gacaacctcc
      661 agacttgatt tttcggaaat tggcggacag ttgccgaagg tcccctccag ctcgatctca
      721 gcccagcagt attctgcctg taactgaagc gaaagaagtt ccgtgatctg cgagagcgtc
      781 tctcgcagcg gaatcgcgct gttgatcccg tcgatcaccc gcgtacgcca gcggtcgatc
      841 gtcgaggccg tggccacacc ctgccttcgt agtttgcgct caagcaggaa tgctttcgcg
      901 atggccccgg ataccaggat caccagaaca cccagcagga taatcaaatt tcgaacggat
      961 acaggggacg gtcctgccag catggcgacg tcctgcgaag accgcagcag caaatgaaaa
     1021 gctgcgggtc cgcgaaattg atcgccgcga tccaggacac atataccggt gacgcgaacg
     1081 cgtgagccca ggggaaaagt gcgcatcggg tcggcgggaa gatccaatcc tcgctccggg
     1141 tgtcggtaga atgcagagaa cacgcgcccc tgcgaggcaa tcacatactg atcctgcgcc
     1201 gcttccccta ccctcatgat gagtcggcct tcgaccgata ccaggttgaa cgcatgggag
     1261 ccggatgcca gttgcaccgg gtccaccttc tccgcgctta gagggacatc cgattgcgtg
     1321 ctttctatct ctgacaaagt aagcacgacc gagccatttc ttacttcggg aaatccggaa
     1381 acaaaagcac ggtctccaat gcgatgcggt tcctcgctga gcgtatctac ccaaagcgca
     1441 ttggcgccgt tctgcaaaac catcgccgct ccgcgctggt agtaggtaat cgtcccttcc
     1501 actctcactc gttcggtttg atcttcaatg tgaagggtat gaagaatttc gttgaacttt
     1561 cgcggcaaaa gctggcccgg actgcgtcgc gccggcgtca caatacgcag gtcggaaaac
     1621 gacggtacct ccagcaggat tccgatcatt tgcgatttgc tgtcgaactt tcccgctacg
     1681 gctccggtga tctcaatcgt gctgtccagc aacgacctca ggtccgtcgt tcctccgttt
     1741 gcaatctgtg cctgaacagg acctcccggc atgagcagat cgaggagtac gctccgcgaa
     1801 ggaccgtcag cgaccaggtt ggctgcacgc acaacggcat gcaccgtcgc ccgccggcag
     1861 tccaggtctc cacggatcag cgtcgcgaaa tccgcggttg ccggcaatgg cggcgtaccg
     1921 tgacggagaa acgtcacgcg tcgtgccaaa atctcgggcc ggaagctcgg tcgcgtgatc
     1981 ccctcaacca gaacgcggtc tccggtcttc aaccccaggt ccggagcggt ctcgacataa
     2041 atggctgcat caccatcctg cacaaaaagg tcgatatttc ccttctggta gtaggtgacc
     2101 gtcccctcaa acagcaccgg gaccgactgt gccgcagttt cattcgagat cgcgtggatg
     2161 gcagacagag aagtcagcgg cgcgcgttgc tgtgcgcaca gagacacagc tacaagcaga
     2221 cagatactgc cagaaagggc ggcgaaactg aaacctcgac caaccatcgt gtaagagcct
     2281 ttagtcgtgc ccgttccatc gttcgcttgc gcaaaaaagc ttttggggtc acgatcgctc
     2341 gataacgtgc cgcaattctg gtgctccccc agggaacctg gcggttcact ttgatttcac
     2401 agcgcatccg ccggcgattg aatgacacaa aggtggcagc cgaacaccga ggagccactg
     2461 gacaagcata ggccctcgtg cccgcgcaaa tcccctttcc agcctcaaac ctccagctcc
     2521 ggtagagcaa gaagcagggc cagccccgcg tggtgccttc cggcgtcggt cttcataatc
     2581 ggttgcggct atactctgaa gttcgtaaag aaacagaggg gcgccaggct gatgttcgtt
     2641 ctgcaggata agtacgtatt tgtttccggg acaaaaaagt gagaacgcct ctcctccgcc
     2701 gagccatcgc gcttccgttg atgcagctaa ccctcgctct ccttgccatc gttctgcccg
     2761 ctgaggctgc ccgatcggga cacagctctg agcggcgagc cgggccggag gtaattgcct
     2821 acgtctttcc tcgagaccac gtcattcaac cggacgaaat cgccccgcgg aaactgaccc
     2881 gcatcaatta cgcctttgcg aatattcagg acggccgcat cgtcgccgtc agtccagccg
     2941 atgccccaaa tctgaccacc ctggttgcgc tgaaacaaca aaacccatct ctgcaaattc
     3001 tgatctccgc agggggatgg ctgtggtcgg gaaatttctc cgatgcagcg cttacacccg
     3061 agagccggtc gcgcttcatt gagagcgcgg ttgccttcat tgaagaacac caactcgatg
     3121 gcctggacat cgactgggag tatccaggcc agatcggcgc cggcaatcgc tttcgccctg
     3181 aagacaagcg gaactacacc cttcttctgg ccgagttacg aaaacgcttt gaacgtgagc
     3241 agaaaaagct gggtcgcccc ctgctgcttt ccatcgccgc cggcgcttcc gacgattttc
     3301 tcgcccatac cgagatgcgc caggtcgctc gtgctgtcga taccgtgaac ctgatggctt
     3361 acgactacta cgagccaggg agcgagccac gcaccggcaa tcatgcgccc ctctacaccg
     3421 atcctgccga cccaaagcac atctccgccg acgcatccgt tcgtcaattc gagagcgaag
     3481 gtgtgccggc gcgcaagatc gttctcggtg tgcccttcta tgggcatgtc tggggaaacg
     3541 tacctccagc caaccatggt ctcttccagc cgggatcgcc ggtgccgaac gcgttcgcac
     3601 gctatcggga catcgtcggc tccatgctgg gacagggctt cacgcgctac tgggatagtt
     3661 ccgcttcggt gccgtatctc tacaacgcgc aaaaagagca gttcgtctcg tatgaggatc
     3721 cccagtccct cgccctgaag tgcgcttatg tgcagagcat gcatctgggc ggagtcatgt
     3781 tctgggagta cgacggcgac gcttccggtg cgctgctgga tac
//
LOCUS       DASZYN010000524         1547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1051226, whole genome shotgun sequence.
ACCESSION   DASZYN010000524 DASZYN010000000
VERSION     DASZYN010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051226"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VHE33_13435"
     CDS             <1..231
                     /locus_tag="VHE33_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98500.1"
                     /translation="PWCNAYFSQQALEDFQSSLAPLGKPSSVKQIADELRGGMTFRAF
                     RVEFPNGGKPLVITTYVEPDGKLEQYLVLPGS"
     gene            356..1357
                     /gene="pstS"
                     /locus_tag="VHE33_13440"
     CDS             356..1357
                     /gene="pstS"
                     /locus_tag="VHE33_13440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HVT98501.1"
                     /translation="MRKSLILATALAVCAAPCLAQHITAAGATFPYPIYSKWFSEYAQ
                     QHPGVQINYQSIGSGGGISQVTKGTVDFGASDMPMTDGMLSQSKVKLMHIPTVLGAVV
                     PVYNVPGVADLKFAPDVLADIYLGKISNWNDARIAKDNPGVSLPNQEIYVVHRSDGSG
                     TTFIFTDYLSKVSPEWKGSVGANSAVAWPKGIGGKGNEGVAGLVRQMPGAIGYVELIY
                     ALQNKISYGSIRNAAGTWLKASIEGVTDAAGSVKTMPADFRVSITNAPGKDAYPISSF
                     TYLLIPTAPGDATKQKVIKDFLTWMLEHGESEVSTLDYAPLPTNVADKVRAAIAKLP"
BASE COUNT          315 a          427 c          492 g          313 t
ORIGIN      
        1 ccgtggtgca atgcgtactt cagccagcag gctctggagg atttccagtc gagcctggcg
       61 ccgctgggga agccgtcgag cgtaaagcag attgcggacg agctgcgtgg cggcatgacg
      121 ttccgcgcgt tccgggtgga attcccgaac gggggtaagc cgttggtcat cacgacgtat
      181 gtggaaccgg atggcaagct ggagcagtat ctggtgctgc cggggagtta gacatggtgt
      241 agttcgacaa cgcctgccgc cgaaagatag ccctttcttc cgcttttcac cgccgcgtaa
      301 acggacgtta acaattcgga cccagaatcg actcagtttc tctgaggggg gattcgtgag
      361 aaagagtttg attctggcca ccgcgctggc cgtttgcgca gcaccgtgcc tggcacaaca
      421 cattactgcg gcgggagcga cgttcccgta tccgatctac tccaaatggt tctcggagta
      481 tgcgcagcag caccctgggg tgcagatcaa ctaccagtcc atcggttccg gcggcggcat
      541 cagccaggtg accaagggga ccgtggattt cggcgccagc gacatgccca tgaccgacgg
      601 gatgctgtcg cagtcgaagg tgaagctcat gcacattccg acggtgctgg gcgccgtggt
      661 tcctgtgtac aacgtgccgg gcgtagccga tctgaagttt gcgccggatg tgctggccga
      721 catctatctg ggcaagatca gcaactggaa cgatgctcgc atcgcaaaag acaaccctgg
      781 cgtgagcctg ccgaaccagg agatctacgt tgtccatcgt tccgacggca gcgggacgac
      841 gttcatcttc accgactatc tgtccaaggt gagcccggag tggaagggca gcgtcggtgc
      901 gaactctgcg gtggcgtggc cgaagggcat tggcggcaag ggcaatgaag gtgtggccgg
      961 cctggtacgg cagatgccgg gtgcgatcgg ctatgtggag ctgatctacg cgctgcagaa
     1021 caaaatttcc tatggatcca ttcgcaacgc ggccggaacc tggctcaagg cgagcatcga
     1081 gggcgtgacg gatgccgcgg gatcggtgaa aaccatgccg gcggatttcc gggtgtcgat
     1141 caccaatgct cccgggaagg atgcttatcc gatctccagc ttcacctatc tgctgatccc
     1201 gacggcgccc ggggatgcga ccaagcagaa ggtgatcaag gacttcctga cctggatgct
     1261 ggaacacggc gagagcgaag tctcgacgct ggactacgct ccgctgccca ccaacgtagc
     1321 ggacaaggta cgggcggcga tcgcgaagct gccgtaaaat ccctggtcgt tactgctgga
     1381 actctcaacg gggcgcgtcc ataagaacga tgcgcccttt tgctttccag gggccgattc
     1441 cggcctgaag caagcttttg cgggctcttt atttccttat ttttcatcac gattaacgtg
     1501 gcattcaaaa tcgccggtta gggtaaacga gtcgtgagtc tgcgcat
//
LOCUS       DASZYN010000525         6931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1051239, whole genome shotgun sequence.
ACCESSION   DASZYN010000525 DASZYN010000000
VERSION     DASZYN010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6931
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051239"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VHE33_13445"
     CDS             complement(<1..584)
                     /locus_tag="VHE33_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HVT98502.1"
                     /translation="MNREYHKWWSWRLDRDMELLVFGHGGVPILVFPSSQGRFFEYED
                     RGLVHAVAHKIDAGQIQLFCVDSVDAESWYKRNVPPRWRIARHMQYENYLLDEVLPLI
                     RLKNLDPHLVALGASFGGYHAVNIAMRHPDRFTGFLSMSGAFDLSNFLGGYYDQDVYF
                     NLPPHYLANLTDPWYFERYSRGTYILGTGWDDHCL"
     gene            689..1567
                     /locus_tag="VHE33_13450"
     CDS             689..1567
                     /locus_tag="VHE33_13450"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HVT98503.1"
                     /translation="MDFQTESLTPTAEQPVEPADPSHPRVTRHAGFSSRFLPGERDIS
                     VYVPPGYDEDSERRYPLLVLQDGQNLFDPSTSFVPGRTWRVAEGADQAIAAGEVEPLV
                     IAGVANAGERRIAEYTPTRDMKLGGGEAGKYALLVIRELLPFLHARYRLLSGPDHTGI
                     GGSSLGGLVSFWMGLAHPEIFGRLAVLSPSVWWNHRYILAYLNDVGPGLPRRPRIWLD
                     AGDEEGRRTLRDAEALRARLRKQGWRDDLDLHFETVAGGTHDEASWAERVQPMLRFLF
                     PGPHSRNKQTFAEDSR"
     gene            1564..1770
                     /locus_tag="VHE33_13455"
     CDS             1564..1770
                     /locus_tag="VHE33_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98504.1"
                     /translation="MSGTPMELIRSLDETARALQQLAHRVRGAAADVADPVLREEMLE
                     SAAGWERRAGEMSEAILRWKREIN"
     gene            1997..3883
                     /gene="dxs"
                     /locus_tag="VHE33_13460"
     CDS             1997..3883
                     /gene="dxs"
                     /locus_tag="VHE33_13460"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HVT98505.1"
                     /translation="MGDLLDSIHSPADLKKLSVPQLESLAQEIRERLIVTLSKTGGHL
                     GPNLGVVELTLALHYVFDTPSDRFVFDVSHQAYVHKMLTGRCDRMETMRQPGGLNGFM
                     LRTESEHDCYGAGHAGTALSAALGMAVARDLAGGNEHVIALAGDAAFTNGISFEALNN
                     IADQTKRLIVVLNDNEWSIDRNVGAIAKYLHKIVTNQHVSQLHESAGRLLARIGGKSA
                     VNVVKRAEEAAKGMLWPSVLFEEFGLTYYGPVDGHNLAQMIESFRFLKQQERPVILHA
                     ITQKGRGFQPALDKQKKFHGLGPYDPETGKTESSGQRTYSEVFAETLVRLASENEKVV
                     AITAAMPNGTALDLFRPHHPQKYFDVGIAEEHAVIFAAGMATRGFRPVCAIYSTFLQR
                     AFDPIVHDVCLQNLPVMFCMDRAGLSGDDGPTHHGLFDIAYLRGVPNIVQMAPTDEDE
                     LADMMYTALLHEGPSAIRYPRGTGPGAKIKQHPQALPLGKAELVQSGDDFVILGLGTL
                     LPMAKELASHLEQQGYSAAVINPRFIKPLDRELIARCARNASAVITFEDHVLMGGFGS
                     AVMEALSEMGIATPVVRIGWPDQFIEHGKVDSLRARHGVSVEAALEKLAPWLRRGAAR
                     LVAQ"
     gene            3956..4480
                     /locus_tag="VHE33_13465"
     CDS             3956..4480
                     /locus_tag="VHE33_13465"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HVT98506.1"
                     /translation="MTTTSSVQLRSSRIRERRDRGIVAIGIFKLLQGALFILLGIGAI
                     RLLHKDLMQVAEHLILAMRFDPEGRFVNLVMDKVALIDPHRLRQISLAIFAVASLDLI
                     EGIGLVLEKAWAEYVTLVLTASFLPWEFFEIIRRATWMRAALLGINLVVVIYLIYYVQ
                     ARMRERRLRKGGVQ"
     gene            4518..5927
                     /locus_tag="VHE33_13470"
     CDS             4518..5927
                     /locus_tag="VHE33_13470"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVT98507.1"
                     /translation="MKRACVIGSGPNGLAAAVVMARAGLPVEVYEAEPIPGGGARTME
                     LTQPGFQHDFGSAVHPMGAGSPFFSSLPLAEFGLQWIHGTSPLAHPLDDGTAVTLEHS
                     LRDQEAQLGEDGVAWRELMEPIATEWPDFALDAMAPPTRIPRRPLLMASFARKAMQPA
                     NALARRIFRGERARALFAGLAAHSFLSFDEPLSPAVGLVLGAAAHVVGWPVPRGGSQA
                     ITNALMGYLEKLNGRVHTSHRITSLDETDPQDGPVLCDLTPRQLLMIAGDRLQRTYRK
                     ALQEFRYGPGIFKIDYALSAPIPWTARECNRAITVHLGGTLEEIARSEFAARHGAVAE
                     KPFVLLVQPSLFDSTRAPEGKHVAWMYCHLPHGSTEDMTGRIEAQVERFAPGFKDCVL
                     ARRTWSPAQLEAMDGNLVGGDIGGGELSMRQFFFRPALGNYYTGTRNLYLCSSSTPPG
                     GGVHGMCGYHAARMALRRL"
     gene            complement(5922..>6931)
                     /gene="ligD"
                     /locus_tag="VHE33_13475"
     CDS             complement(5922..>6931)
                     /gene="ligD"
                     /locus_tag="VHE33_13475"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HVT98508.1"
                     /translation="PHHDAKKAKHSVPAGTAAKVESPRPSKSPNRTQVAGLSITHPDK
                     ILDESSHLTKLQLVEYYDAVADHLLPHIADRPLSIVRCPEGSGKPCFFQKQIGKGMPD
                     GVDSVPVTVKKGGNPQREEYVTVSRREGLVGLGQMGVMELHPWGSSNETLEQPDRIII
                     DLDPDPELPWKRLVESAIEVRDLMKHLGLETFVKSTGGKGIHVVAPIEPEREWPEIKE
                     FTHNFVLMMERSNSALYLTKMTKSARTKRIFLDYLRNERGATAVAPYSPRARAGVRVA
                     MPLTWKELEHTDPKQFAVANFDAWKARLKHDPWTKMNQVRQKISDRAVAAVAEMAGQK
                     S"
BASE COUNT         1285 a         1934 c         2321 g         1391 t
ORIGIN      
        1 aggcagtggt cgtcccagcc ggttccaagg atgtaggttc cgcggctgta gcgctcgaag
       61 taccaggggt ctgtgaggtt tgccaggtag tggggtggca ggttgaagta gacgtcctga
      121 tcgtagtagc cgccgagaaa gttgctcagg tcgaaggctc cgctcatcga gaggaatccg
      181 gtgaagcgat ccggatggcg catggcgatg ttcaccgcat ggtaaccgcc aaagctggca
      241 ccgagcgcga ccagatgcgg gtcgagattc ttcaaccgga tcagcggcag cacctcgtcc
      301 agcaggtagt tctcgtactg catgtggcgc gcaatgcgcc agcgcggcgg cacgttgcgc
      361 ttgtaccagc tctcggcgtc aaccgagtcg acgcagaaaa gctgaatctg gcccgcgtcg
      421 atcttgtgcg cgacggcgtg gacgagcccg cgatcctcgt actcgaagaa ccggccctgc
      481 gaggagggaa agacgaggat gggaacgccg ccatggccga agacgagcag ctccatgtcg
      541 cgatccagac gccacgacca ccatttgtga tattcgcggt tcatagcttg ttcacgcagc
      601 cgttacggta tcgtaaagca tcggaatgca gagagcgcgg gagaatccgc cgaagcgcca
      661 cagcgacacc aggagagatc aggaaccgtt ggacttccag accgagagcc tgacgccgac
      721 tgcggagcaa cctgtcgagc cggcggaccc gtcccatccg cgcgtcacca ggcatgcggg
      781 gttttcgagc cgctttctgc caggggagcg cgacatcagc gtctacgtgc cgcccggata
      841 cgacgaagat tcggagcggc gctatcctct gctggttttg caggacggcc agaacctgtt
      901 cgacccctcg acgtcgttcg ttccgggaag aacgtggagg gtggccgaag gcgccgacca
      961 ggccatagca gcaggggaag tggaaccgct ggtgattgcg ggtgtcgcga atgccggcga
     1021 gcggcgcatc gctgagtaca caccgacccg agacatgaag ctgggcggcg gcgaggcagg
     1081 gaagtacgca ttgctggtga ttcgggaact gctgccgttc ctgcacgcgc ggtatcgttt
     1141 gctcagtggg cccgatcaca ccggtatcgg tgggtcgtcg ctgggcgggc tggtttcgtt
     1201 ctggatgggc ctcgcgcatc cggagatttt tggccgtctc gctgtgttat cgccctcagt
     1261 gtggtggaat catcgctaca tcctggcgta tctgaacgac gttggtcctg gcctgccgcg
     1321 gcggccgaga atctggctcg atgcaggcga cgaggaaggg cgccgcacgc tcagagacgc
     1381 agaggcgctg cgcgcgcggc tgcgcaagca aggctggcgc gacgatctgg acctgcattt
     1441 tgaaacggta gccggaggca cacacgatga agcctcctgg gccgaacggg tccagcccat
     1501 gctgcggttc ctttttccgg gcccgcacag caggaacaag cagacgtttg cagaggattc
     1561 gcgatgagcg ggacaccgat ggagctgatt cgcagcctcg acgaaactgc gcgggccctt
     1621 cagcaactgg ctcatcgggt gcgcggcgca gctgcggatg tcgccgaccc ggttctgcgc
     1681 gaggagatgc tggagagtgc tgccggctgg gagcgacgcg ccggggagat gtccgaagcg
     1741 atcctgcgct ggaagcgcga gatcaactaa tccgcgctcc agcgctacgc gacatggcgc
     1801 cggaacgcct gaaaacgcgg gcccgccaaa cccggtatac ttacagttca gaggatgttc
     1861 cggaccagaa ggccctgact gccgtcccgc cgtgcaagtg cgcaggctgc ggattccggc
     1921 tgtgggtctt ttttgattcc gggactgtca tccgcgcggc tcggatgttc ttgaacgcgg
     1981 accctctgga accctcatgg gcgacctact cgacagcatc cactctcccg ccgatctgaa
     2041 gaaattatct gtcccgcaac tggaatctct tgcacaggag attcgggagc ggttgatcgt
     2101 cactctttcg aagaccggcg gccatttggg gccgaatctg ggcgtggttg aactgacgct
     2161 cgccctgcat tatgtcttcg atactccgtc ggacaggttt gtattcgatg tgagccacca
     2221 ggcgtacgtg cacaagatgc tgacaggacg ctgcgaccgc atggagacga tgcgccagcc
     2281 gggcggattg aacggcttca tgctgaggac ggagagcgag cacgattgct atggcgccgg
     2341 acatgcgggt acggccttgt cagcggctct gggcatggcg gtggcgcgcg atctggccgg
     2401 aggcaacgaa cacgtgatcg cgctggcggg cgacgcggcc ttcaccaacg gcatttcctt
     2461 cgaggcgttg aataatattg ccgatcagac gaagcggctg atcgtggtgc tgaacgacaa
     2521 cgagtggtcg atcgatcgca acgttggtgc gattgcgaaa tacctgcaca agattgtgac
     2581 caaccagcat gtgagccagc tgcacgagag cgcgggacgg ctgctggcac gcatcggcgg
     2641 caagagcgcg gtcaatgtgg tgaagcgtgc cgaggaagcc gcgaagggca tgctgtggcc
     2701 atcggttttg ttcgaggagt tcggactgac gtactacggt ccggtggatg gacacaatct
     2761 tgcgcagatg atcgagagct tccgcttcct gaaacagcag gagcgtccgg tgattctgca
     2821 tgcgatcacg cagaagggcc gtggattcca gccggcactg gacaagcaga agaagttcca
     2881 tgggctgggg ccttacgatc cggaaaccgg gaagaccgag tcgagcgggc agcggacgta
     2941 ctcggaagtc ttcgccgaga cgctggtgag gttagcgagc gaaaacgaga aggtcgtagc
     3001 gatcaccgcg gccatgccca atggcacggc gctcgatctt ttccggccgc atcatccgca
     3061 gaagtatttc gatgttggca tcgctgagga acatgcggtg attttcgcgg cggggatggc
     3121 gacgcggggt ttcaggccgg tttgtgcgat ctactccacg tttctgcagc gggcctttga
     3181 cccgatcgtc catgacgtgt gcctgcagaa tctgccggtg atgttctgca tggaccgcgc
     3241 aggattgagc ggcgatgacg gtccgacgca tcacggcctg ttcgatattg cgtatctacg
     3301 cggcgtaccg aacatcgtgc aaatggcgcc gacggatgaa gatgagctgg ccgatatgat
     3361 gtacacggcc ctgctgcatg aggggccaag cgcgatacgc tatccgcgtg gcacgggacc
     3421 gggcgcaaag atcaagcagc atccgcaggc gctgccactg ggcaaggcgg agctggttca
     3481 aagcggcgat gattttgtga ttctgggtct gggcacactg ttgccgatgg caaaggaact
     3541 tgcctcgcat ctggagcagc aggggtactc cgccgcggtc atcaacccgc gattcatcaa
     3601 gccgctcgat cgggagctga tcgcgcgttg tgcacggaac gcgtcggcgg tgatcacgtt
     3661 tgaagatcac gtgctgatgg gcggattcgg gagcgctgtg atggaagctc tcagcgagat
     3721 gggcattgcg acacctgtgg ttcgcatcgg gtggccggac cagttcatcg agcacggcaa
     3781 ggtcgattcg ctgcgtgctc gtcacggtgt aagcgtagaa gcggcgctgg agaagcttgc
     3841 tccctggctc cgacgagggg cagcccggct tgtggcgcaa tagatgtagg tgtttcgttg
     3901 gcgtcctttc gcggcggtgt gggaagatgc ggagagaacc caacggcttc gttctatgac
     3961 aacgacctcc tccgtccagc ttcgatcctc gcgcattcgc gagcgccgcg atcgcggtat
     4021 cgtcgctatc ggcatcttca agctgctgca gggggccttg tttatcctgc tcggaattgg
     4081 cgccatccgc ctgttacaca aagacctcat gcaggtggcc gaacatctta tcctcgcgat
     4141 gcgcttcgac ccggaaggcc gatttgtgaa cctggtgatg gacaaggtag cgctgatcga
     4201 tccgcaccgg ctgaggcaga tcagccttgc catcttcgcg gttgcgtcgc tggatctgat
     4261 tgagggaatc ggcctggtgc tggagaaggc atgggcggaa tatgtgaccc tggtcctgac
     4321 agcttcattt ctgccctggg agttcttcga gattatccgc cgagccacat ggatgcgagc
     4381 cgcacttctg gggatcaacc tggtcgtggt gatctacctc atctattacg tacaagcgcg
     4441 catgagagaa cggcgactgc gaaaaggcgg agttcagtga tgcaggaacg gcgaggttcc
     4501 ggcgcggggg gacgtgcatg aagcgagcct gcgtgatcgg atcgggtcca aacggcctgg
     4561 cagcggcggt tgtgatggcg cgcgcagggc tgccggtcga agtgtacgaa gcggaaccga
     4621 tcccgggtgg cggggcccgc accatggagc tgacacagcc cgggttccag cacgattttg
     4681 gatcggcggt acatccgatg ggcgccgggt cgccgttttt ctcgtctctc ccgctggctg
     4741 aattcggtct gcagtggatt catggcacgt caccgctggc gcatccgctg gacgacggta
     4801 cagccgtgac gctggagcac agcctgcgcg atcaggaagc acagcttggt gaggacggtg
     4861 ttgcctggcg tgagctcatg gagccgattg cgaccgaatg gccggatttt gctctggatg
     4921 cgatggcccc gccaacccga attccgcggc ggccactgct gatggccagt tttgcgcgca
     4981 aggcgatgca gccggccaat gccctggcgc gacggatctt tcgcggcgag cgggcgcggg
     5041 ccctgttcgc aggactggcg gcgcattctt tcctcagttt cgatgagccg ttgagcccgg
     5101 cggtgggtct ggtgctggga gccgcggccc acgtggtggg gtggccagtt ccgcgcggcg
     5161 gatcgcaggc gatcaccaat gctctgatgg gatacctgga aaagctcaat ggccgcgttc
     5221 acaccagtca tcggatcacg tcgctggacg agacggatcc gcaggatggt ccggtgctct
     5281 gcgatttgac accgcggcaa ttactgatga ttgccggcga tcggctacaa agaacctatc
     5341 gcaaagccct gcaggaattt cgatacggtc cgggcatttt caagattgat tacgcgcttt
     5401 cggcaccgat cccgtggact gcgcgcgagt gcaatcgggc gatcacggtg catctggggg
     5461 gaacgctgga ggagattgcg cgatcggagt ttgccgcaag gcatggtgcg gttgcggaga
     5521 agccctttgt gttgctggtg cagccgtcgc tcttcgattc cacgcgtgcg cccgaaggaa
     5581 agcacgttgc ctggatgtac tgccatcttc cgcacggatc gaccgaggat atgacaggcc
     5641 gcattgaggc gcaggtggag cgtttcgcac cggggtttaa ggattgcgta ctggcacgcc
     5701 ggacatggtc gccagcgcaa ctggaagcga tggacggcaa tctggttgga ggggacatcg
     5761 gtggcggcga attgtcgatg cggcagttct tcttccgccc cgctctcggg aactactaca
     5821 ccggtacgcg caatttgtat ctgtgttctt cgtcgacgcc gcccggcgga ggggtccatg
     5881 ggatgtgcgg ctaccacgcc gcccgcatgg ccttgcggcg gttatgattt ctgccccgcc
     5941 atctcggcta cagcggctac agctcggtcg ctgatcttct ggcgcacctg attcatcttt
     6001 gtccagggat catgcttgag gcgcgccttc cacgcatcga aattcgcaac ggcgaactgt
     6061 tttggatccg tgtgctccag ttctttccag gtgagcggca tggcaacgcg aacgccggcg
     6121 cgggcacggg gagaataggg cgcgacggcg gtagctccgc gttcattgcg taaatagtcg
     6181 aggaagatcc gctttgtgcg ggcggacttg gtcattttgg tcaggtatag cgcggagttg
     6241 gagcgctcca tcatgagcac aaagttgtga gtgaattctt tgatctccgg ccactccctc
     6301 tctggctcga tgggagcgac gacgtgaatg cctttgccgc cggtcgattt gacgaaggtc
     6361 tcgaggccga ggtgcttcat caggtcgcga acttcaatgg cgctttctac caggcgtttc
     6421 cagggcagct cgggatccgg atcgaggtcg atgatgatgc gatcgggctg ctccagagtc
     6481 tcgttgctgg atccccacgg gtgtaattcc atgacaccca tctggccgag gccgacgagg
     6541 ccttcgcgcc tggagaccgt gacgtattcc tctcgttgcg gattgccgcc cttcttcacc
     6601 gtgaccggca cgctgtccac gccgtccggc atgcccttgc cgatttgctt ttggaagaag
     6661 caaggtttac cgcttccttc cgggcagcgg acgatgctca gcgggcgatc agcgatatgg
     6721 ggcagcagat gatcggcaac ggcatcgtag tactccacca gttgcagctt ggtcaggtgc
     6781 gatgactcgt caagaatctt atcgggatga gtgatggata gcccggccac ctgggtacgg
     6841 ttgggtgatt tggagggtct ggggctctca actttcgccg cagttcctgc aggcacagag
     6901 tgtttcgcct tttttgcatc gtgatgcggt g
//
LOCUS       DASZYN010000526         5679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_928343, whole genome shotgun sequence.
ACCESSION   DASZYN010000526 DASZYN010000000
VERSION     DASZYN010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5679
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928343"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VHE33_13480"
     CDS             <1..402
                     /locus_tag="VHE33_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98509.1"
                     /translation="DVHGETVQLLNERFPYWFCGNFKRYNGRDAELPFDQNEVLALIA
                     PRPLYIASAILDGNADPQGEFLGALAVTRVYRFLGSSGLPAAKWPAVNQPLLGRVSYH
                     VRSGEHSVTAFDWEQYLRFCDRSVKAAAKSQ"
     gene            complement(586..1338)
                     /locus_tag="VHE33_13485"
     CDS             complement(586..1338)
                     /locus_tag="VHE33_13485"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HVT98510.1"
                     /translation="MPKSDTHMTMRVVNHIRGMIESGELQPGDRIPPEREFARKLRIS
                     RASLRTGIGYLAAMGVMDVRHGVGTFVANGPPELGKSSLSLLGALHGFQSWQMFEARR
                     ILESELAALAAERGREQQFAALSEEVAEMYATVDNPMEYLIHDVRFHRVIAQASGNPI
                     LAALMETITSALYDERRKTAERSRDLKESADLHREIYRAIRSRNAAEARSVMERHLMR
                     AQSAQDLEIGGPPPVLRSENGRTKAGRRRDQA"
     gene            1531..2967
                     /gene="uxaC"
                     /locus_tag="VHE33_13490"
     CDS             1531..2967
                     /gene="uxaC"
                     /locus_tag="VHE33_13490"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF002794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HVT98511.1"
                     /translation="MSTRAMALIHDDRLFPADPTMRSIARRLYAEVRDLPLICPHGHT
                     QASWFAKDQPFPNPAQLFVQPDHYVFRMLYSQGIPMEDLEIGAPEIKDPRRVWRIFAE
                     NYSLFRGTPTRIWLDYAFHELFGLDERLSAATADLYYDVIAERLTTPEFRPRALYERF
                     RIEVLCTTESPLDPLAAHQAVRDSSWKPRGAAARIVPAFRPDPVVDPAFRGFAENLAK
                     LGQMTGEDTGAWKGYLRALEKSRARFKALGCTSTDHGHPTALTADLPLAEASALYDAV
                     RLGRADAKQEELFRAQMLTEMARMSVEDGLVMQLHPGSFRNHNRKIYEKYGRDVGADI
                     PVATDYVRALHPLLERFGNEKKLTLILFTLDETTYTRELAPLAGHYPCLRLGPPWWFH
                     DSPEGMLRFREQVTETAGFYNTVGFNDDTRAFLSIPARHDVARRIDCVWLARLVAEHR
                     LDEDEAVEVAHDLAYNLVRKAYKLEPGS"
     gene            3043..4350
                     /locus_tag="VHE33_13495"
     CDS             3043..4350
                     /locus_tag="VHE33_13495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT98512.1"
                     /translation="MATRSPESRSFFGTFGSPDRVTHQRWVICALLFFATVIAYVDRG
                     VIAYLEKFLEGIIPGLNSVKYGYILAAFQAAYAIGMVVAGGLTDKLGTRKSFAIAIGL
                     WSVAAMLPGAAFSVVTFGIAMFLLGLGEAANFPACIKTVAEWFPKRERALATGIFNSG
                     ANIGNIVVPLVVPALVVVVGWRGAFVVTGGAGIVWLICWLIYYRRPEQHRSVSKAELD
                     LILSDPVEKLESVPWSRVSPRKETWAFAIGKFLTDPIWWFYLFWLPRYLQQTFGLSLA
                     SNRLPLVIIYSVSTVGSIGGGWISSTLLNSGKTANVARKTAMLICALCVLPVFAAPYI
                     HHLWVVVGLVGLATAAHQGWSANLFTLPSDMFPKAAVASVIGIGGMAGAIGGVLMQLA
                     TGYIVKLTNSYVPLFVIACLAYVVALAIIHGITPKLAPARIES"
     gene            4369..5643
                     /locus_tag="VHE33_13500"
     CDS             4369..5643
                     /locus_tag="VHE33_13500"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HVT98513.1"
                     /translation="MSLYCNVGSADTDLSGQKLNDLLVQTLDKLGPRKRVLVIPPDIT
                     RLHSQAGELTQFAWKYYGDRLAAVLPALGTHAAMSSEQLTRMFGDMPQSLFHIHNWRT
                     DIETIGELPPSFIEEQSEGKLHYSWLAQVNRLLLNGNFDLILSVGQVVPHEVIGMANY
                     NKNVLIGTGGREGINRSHYLGAVYGMERIMGRADNPVRRVLNTASDRFLKNLPIVYAH
                     TVVGRGADGGLVTRGFYVGDDVACFHKAAELSLQVNFEMLDEPVRKAVVFLDPHEFRS
                     TWLGSKSIYRTRMALADGAELIILAPAVKEFGEDRTIDALIRKYGYRGTPATLEAVAK
                     NADLAADLSAAAHLIHASSEGRFTITWCPGHLTREEVEGVGFSYGDLAEMQRRYDPAK
                     LKHGFNNVEGEEVFFVANPGLGLWAHRDRFKD"
BASE COUNT         1028 a         1650 c         1838 g         1163 t
ORIGIN      
        1 gacgtgcacg gggagacggt gcaactgctg aacgaacgat tcccctactg gttctgcggg
       61 aatttcaaac ggtacaacgg ccgggatgca gagttgccct tcgaccagaa tgaggtgctg
      121 gcgctgattg ctccccgacc actctatatt gcgagcgcga tcctcgacgg gaatgccgat
      181 ccgcagggtg aattcctggg cgcgctggcc gtcacgcggg tctatcgatt tctgggaagc
      241 tcaggcctgc ctgcggcgaa atggcccgct gtgaaccagc cgctgctggg gcgggtgagc
      301 taccacgttc gcagcgggga gcatagcgtg acggcgttcg attgggagca gtatctgcgg
      361 ttctgcgatc gatctgtaaa ggctgccgcg aagagccagt gatgcgcagc ggcttgtcca
      421 aaactatggc gtagcccgga cgaaaccatg acgcatcgat ccgccgtttt aagatcgccc
      481 atcgctcgtc ctgcataggc tatctgcgct aagaggaggg aagacccgtt tcccctcccg
      541 aaatagagcc tgagcagagg gcacgacagc gacaaagatg gtcggttagg cctggtcccg
      601 ccgccgtccg gctttcgtgc ggccattctc ggagcgaagc acgggaggcg gcccgccaat
      661 ctcgaggtcc tgggcggact gggcgcgcat gagatggcgt tccatgacgg aacgggcttc
      721 agcggcgtta cgggaacgaa tggcccggta gatttcgcgg tgcagatcgg cagattcctt
      781 aagatcgcgg gagcgctccg ctgttttgcg gcgctcatca tagagagccg aggtgatcgt
      841 ctccatcagc gcggcgagga taggattgcc cgaggcctgg gcgatgacgc gatggaagcg
      901 cacgtcgtga atgaggtatt ccatcggatt gtcgacggtg gcgtacattt cggcgacttc
      961 ttcggagagg gcggcaaact gctgctcccg gccgcgctcg gcggccaggg cggcaagttc
     1021 gctctcgagg atgcggcgcg cctcgaacat ctgccaggac tggaaaccgt gcagcgcgcc
     1081 cagcaggctc agagaggatt tgcccagctc cggtggtccg ttggcgacga aggtgcccac
     1141 tccgtggcgg acatccatca cccccatggc cgccaggtag ccgattccgg tgcgcaggct
     1201 ggcgcggctg atgcgcagct ttcgcgcgaa ttcgcgttcc ggcggaatcc ggtcgccggg
     1261 ctgcaattcg ccggattcga tcatgccgcg gatgtggtta accacccgca tggtcatgtg
     1321 cgtatccgat ttcggcatgg cctcctcagc cgggaggttc agttcctgcg cttggctgtt
     1381 cctcattccg agatttacag taccttacgc tgcatttgtt gtaaggtcca acaaaagaac
     1441 ggctgaattc taaagactgc ggtgtatcct agtggtcgga ccacgacgcc gcagtgctga
     1501 ttctgcgagt gcaggaggtc aaccagtccg atgtccaccc gtgctatggc tctgattcat
     1561 gacgatcgcc tgtttccggc tgatcccacc atgcgcagca tcgcgcgccg cttgtacgcc
     1621 gaagtgcggg atctgcccct gatctgtccg cacggccata cccaggcctc ctggtttgcc
     1681 aaagaccagc cgtttcccaa cccggcccag ctctttgtgc agcccgatca ctacgtcttc
     1741 cggatgctct acagccaggg cattcccatg gaagacctcg aaatcggcgc cccggaaatc
     1801 aaggatccca ggcgcgtttg gcgcattttc gcggagaact actctctgtt ccggggaacg
     1861 ccaacgcgga tttggctgga ttacgcgttc catgagctct tcgggctgga cgagcggctg
     1921 tccgccgcga cggccgatct gtattacgac gtgatcgcag agcgcctgac gacgccggag
     1981 ttccggcctc gcgcgctgta tgagcggttc cgcattgagg tgctctgcac cacggagtcc
     2041 ccattggatc cgctggccgc tcatcaggcg gttcgtgatt ccagctggaa gccgcggggc
     2101 gccgcagcgc gaatcgtgcc ggcgtttcgg ccggacccgg tggtggatcc ggcgttcagg
     2161 ggctttgcgg agaatttggc gaagcttggc cagatgaccg gcgaagatac cggcgcctgg
     2221 aaggggtatt tgcgtgcgct ggaaaaatcg cgagcgcgct tcaaggcgct ggggtgcacg
     2281 tcgacggatc acgggcatcc gacggcgctc acggcggatt tgccgctggc ggaagcctcg
     2341 gctttgtacg acgctgttcg cctgggacgt gccgacgcga agcaggagga gctgttccgg
     2401 gcccagatgc tcaccgagat ggcgcggatg agcgtggaag acggcctggt gatgcagctg
     2461 catccgggct cgttccgcaa tcacaatcga aagatttacg agaagtacgg gcgcgatgtt
     2521 ggcgcggaca ttccggtcgc tacggattac gtgcgtgcgt tgcatccgct gctggagcgc
     2581 ttcggcaatg agaagaagct gaccctgatc ctgttcacgc tggacgagac gacctataca
     2641 cgcgagttgg ccccgctcgc cggacactat ccttgcctgc ggctgggacc gccgtggtgg
     2701 ttccacgaca gccccgaggg gatgctgcgc ttccgcgaac aggtcacgga gactgcaggc
     2761 ttctacaaca ccgtcggctt caatgacgac acgcgcgcgt tcctgtcgat ccctgcgcgg
     2821 cacgacgtgg cgcgccgcat cgactgtgtg tggctcgcgc gactggtggc cgagcatcgc
     2881 ctcgacgagg acgaagcggt ggaggttgcg cacgatctcg cctacaatct ggtgcggaaa
     2941 gcctacaagc tcgaaccggg cagttagcgg tcagcagtca gcggcatccc tgcatttcca
     3001 gcagggcggg tctggatggg gaccgcgagt atcggagatt gaatggcgac aagaagcccg
     3061 gaatcacgct cgtttttcgg cacctttggc tccccggatc gcgtaaccca ccagcgctgg
     3121 gtgatctgcg cgttgctgtt ctttgcgacg gtcatcgcct acgtcgatcg tggtgtgatc
     3181 gcctatctcg aaaaattcct cgaagggatc atccccggac tcaacagcgt caagtatggc
     3241 tatattctgg ccgcgtttca ggccgcgtac gcaatcggga tggttgtagc cggtggattg
     3301 accgacaagc tgggcacccg caagtcgttt gcgatcgcca ttggcttatg gagcgtcgct
     3361 gcgatgctgc ccggtgcggc attttccgtc gttacgtttg gcattgccat gttcctgctc
     3421 ggcctgggcg aagcagcgaa ttttcccgcg tgcatcaaga ccgttgccga gtggttcccc
     3481 aaacgtgaac gcgcgctggc cacaggcatc tttaattccg gcgcaaacat tggcaacatt
     3541 gtcgtgccgc tcgtcgttcc cgccctggtt gtggtggtcg gttggcgcgg cgcgtttgtc
     3601 gtcaccggcg gcgccgggat tgtgtggctg atttgctggc tgatctacta tcgccggccg
     3661 gagcagcacc gcagcgtttc gaaagcggaa ctggatctga ttctcagcga tccggtggaa
     3721 aagctcgaaa gcgttccgtg gagccgggtc tcgccccgca aagaaacctg ggcctttgcc
     3781 atcggcaaat ttctcaccga tcccatctgg tggttctacc ttttctggct gccgcgttat
     3841 ctccagcaaa catttgggct tagcctggct tcgaaccgcc tgccgctcgt gattatctac
     3901 agcgtttcga ctgtgggcag catcggcggc ggatggattt cttccacgct gctgaattcc
     3961 ggcaaaaccg caaatgtcgc ccgcaaaact gcgatgctga tctgcgcgtt gtgcgtgctg
     4021 cccgtgtttg cagcgcccta tattcatcac ctttgggtgg tggtgggcct ggtgggtttg
     4081 gcgactgctg cccatcaggg atggtcggcg aatctgttca cgctcccgtc cgatatgttt
     4141 cccaaggcag cggtggccag cgtaatcggc attggaggca tggcgggggc catcggcggt
     4201 gtgttgatgc agttggcgac cggatacatc gtcaagctga ccaacagcta tgtgccgctc
     4261 ttcgtcattg cctgcctagc gtacgtcgtc gcgctggcga tcattcacgg gattacgcca
     4321 aagctggcgc cggcccggat cgagtcgtaa agggaggagg gatcgcagat gagtctctat
     4381 tgcaacgtgg gcagcgctga tacggacctc tccgggcaga agctgaacga tctgttggtg
     4441 cagaccctcg acaagctggg gccgcgcaag cgggtgctgg tgattccgcc ggatatcaca
     4501 cgcctgcact cccaggcggg cgagctcacg cagttcgcgt ggaaatacta tggcgatcgg
     4561 ctggcggcgg tgttgccggc gctgggcacg catgctgcga tgtcatcgga gcagctcacg
     4621 cgcatgttcg gcgatatgcc acagtcgctg ttccatattc acaactggcg aacggacatt
     4681 gaaaccatcg gggagttgcc tccttcgttc atcgaggagc agtcggaagg caaactgcac
     4741 tactcctggc tggcccaggt caaccggctg ctgctcaacg gaaatttcga tctgatcctt
     4801 tcggtggggc aggtcgtgcc gcatgaagtc atcggcatgg ccaactacaa caagaacgtg
     4861 ctgatcggga cgggcggacg agaaggcatc aaccgcagcc actatctggg cgccgtgtac
     4921 gggatggagc gcatcatggg ccgcgccgat aatcccgtgc ggcgggtgct gaataccgct
     4981 tcggaccgct tcctgaaaaa cctgcccatc gtttacgcgc atacggtcgt cgggcgtggg
     5041 gcggacggcg gtttggtgac gcgcggattc tatgttggcg atgacgttgc ctgcttccac
     5101 aaggcggcgg aactgagcct gcaggtgaat tttgagatgc tcgacgagcc ggtgcgcaag
     5161 gcggtggtgt ttctcgatcc gcatgagttc cgcagcacgt ggctcggcag caagtccatc
     5221 taccgtacgc gcatggcgct ggcggacggt gcggagttga tcatcctggc gccggcggtg
     5281 aaggaattcg gcgaggatcg gacgattgac gcgctgatcc gcaagtacgg ctaccgcggg
     5341 actccggcga cgctggaggc tgtggcgaaa aatgccgatc tggccgcgga cctaagcgcc
     5401 gccgcacatc tgatccacgc ctcgtcggag ggccgcttca cgatcacgtg gtgccccggc
     5461 catctgacgc gcgaagaagt ggaaggcgtg ggattcagct acggcgacct tgccgagatg
     5521 cagcgtcgct acgatcccgc aaagctgaag cacggtttta acaatgtgga aggcgaagag
     5581 gtgttcttcg tcgcaaaccc gggcctggga ctctgggccc atcgcgatcg atttaaagac
     5641 tgaggtactt atggcaacga cggaactcgg aaaattttc
//
LOCUS       DASZYN010000527         2237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_991037, whole genome shotgun sequence.
ACCESSION   DASZYN010000527 DASZYN010000000
VERSION     DASZYN010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991037"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VHE33_13505"
     CDS             <1..189
                     /locus_tag="VHE33_13505"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HVT98514.1"
                     /translation="PSAVITLKQGFGRLIRSLDDRGVLMLLDPRLERQRYGRIFLESL
                     PPYRITQNIADVEAFFSR"
     gene            complement(315..614)
                     /locus_tag="VHE33_13510"
     CDS             complement(315..614)
                     /locus_tag="VHE33_13510"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HVT98515.1"
                     /translation="MAHRLAPEAEDELDNIWLYVAKESGSVEIADRLIDSVTNTFYLL
                     ASHPHIGRARDVDLRAGLRSFALGQYVIIYRIERSDVLILHVFRGSRDIEALLRQ"
     gene            complement(614..895)
                     /locus_tag="VHE33_13515"
     CDS             complement(614..895)
                     /locus_tag="VHE33_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98516.1"
                     /translation="MDVQLTSDQKAFIRQAVESGRLLREEDAIQEALSLWEERERGRA
                     EILTAVDLAEASLAGGAGRPITRNSMLELADDVKQRGRRRLATESSSSR"
     gene            1165..1863
                     /locus_tag="VHE33_13520"
     CDS             1165..1863
                     /locus_tag="VHE33_13520"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HVT98517.1"
                     /translation="MERNQGCMDCPARGKHMFCSMKETEMGKFDTLGTQVRFSTGAVI
                     FREGDNCGSVHVLCSGRVKLSATSREGRTLILKIARAGEVLGLSAALAGQPFEVTAET
                     VEPCTLKTIRRQALLDFLDRHPDASMRAARAVAQEYKAAFSEVRRLALPATASGRVAN
                     LLLDWSREQAPEHAANGRCMMPLTHEEIASMTATTRETVTRVLGQLKRDQLISIRGAS
                     LTVLQPQALERLAV"
BASE COUNT          437 a          774 c          620 g          406 t
ORIGIN      
        1 ccctccgccg tcatcacgct caagcagggc ttcggccgcc tcatccgctc cctcgacgac
       61 cgcggcgttc tcatgctcct cgacccgcgc ctcgagcgcc agcgctacgg ccgcatcttc
      121 ctcgaaagcc tgccccccta ccgcatcacc caaaacatcg ccgacgtcga agccttcttc
      181 agcagatagt tcttcccctt gtcatcccga ccgaagcgga gggacctgct ttggttccgc
      241 ataaagaaaa ttcggaagca ccccacgagc ggggaagtga aagaagaaag gaagcggaat
      301 caggaggact acgcttactg ccgcaacaac gcctcgatat cccggctgcc gcgaaaaacg
      361 tggaggatca acacatcgct ccgttcaatg cggtagatga tgacatattg cccgagagca
      421 aagctgcgca gcccggcacg cagatccaca tcgcgcgcgc gtccgatgtg cgggtggctt
      481 gccagcaggt agaaggtgtt cgttaccgag tcaatcaacc gatcggcgat ctcgacactg
      541 ccgctttcct tcgcgacgta gagccagatg ttgtcgagtt catcttcggc ttccggcgcc
      601 aggcggtgag ccatcagcga gaagaggatg attcggtggc cagacggcgc cgtccgcgct
      661 gcttcacatc gtcggccagc tcgagcatag aattccgggt gatcggtcgc cccgcgccac
      721 cagccaggga agcctcggcc aggtccaccg cagtcaggat ttccgcgcgg ccgcgctcgc
      781 gctcttccca gagggaaagc gcctcctgaa tggcgtcttc ctcacggagc aggcgtccac
      841 tctcgacagc ctggcggata aatgcctttt gatcggaagt gagttggacg tccatacgct
      901 tgagcgtaag gtcgggccga gcgagtgtca atcataggcc ggtatgactc tgagctcatt
      961 gcagtcggct tctccctcct cctgcgccaa accgcgcaat cctccaggct gtgtgacgca
     1021 gcgcacattc caccgcgcac ccccgcgcac aggtgatcct cagcaccaac acccgtgatt
     1081 tcaatcacat gtcgattgcc ccgcactttg gcccgccccg tgcaataagt cccggtgtca
     1141 gtcaacaccc caggagatac gaccatggaa cggaatcagg gatgcatgga ttgcccggcg
     1201 cgcggtaagc acatgttctg cagcatgaaa gagacggaga tgggaaagtt cgacaccctt
     1261 ggaacccagg tccgcttcag caccggcgcg gtcatcttcc gcgaaggcga taactgcgga
     1321 tcggtgcacg ttctctgctc cggacgcgtg aagctctccg ccaccagccg cgaaggccgc
     1381 accctgatcc tcaagatcgc tcgcgccggc gaagtcctcg gcctctccgc cgctctggcc
     1441 ggccagccct tcgaagtcac cgccgagacc gtggagccgt gcacgctcaa gaccatccgc
     1501 cgccaggcgc tgctcgattt tctcgatcgc caccccgacg ccagcatgcg cgccgcgcgc
     1561 gccgtcgccc aggaatacaa agcggctttc agtgaagtcc gccggttagc cctccccgcc
     1621 accgcctccg gccgcgtcgc caatctgctg ctcgactgga gccgcgaaca agcccccgag
     1681 cacgccgcca acggccgctg catgatgccc ctcacccacg aagagatcgc atccatgacc
     1741 gccaccacgc gcgaaaccgt cacccgcgtc ctcggccagc tcaagcgcga tcaactgatt
     1801 tccatccgcg gcgcctcgct caccgttctg caaccacagg cgctcgaacg gctcgcggtg
     1861 taacaagcca ggccctcccc ctggagctgt gaattttcct tgctgtactt gcagtgatcc
     1921 tcccgaggca gcccgcaaag gctgcctcct ttatttgcgt tgaggaaggg ttcgggtgtc
     1981 cacaacgccg catccggctg agtccgccga aaatccccaa tgtccgagat gccgactttt
     2041 ccgtcaggac ttcgctgagc gcattggcat gaagtcaccc catgacggcc aacgtcgcga
     2101 acgcagtcgt ctgctagact gttgagaaat tttcgccggt gaggtgatcg atgcgtctcc
     2161 gctctgtttg caccgctttc attctgggag ggtgtgttct ggcgagcact gcacaaacgg
     2221 gctcggcgca aacgggg
//
LOCUS       DASZYN010000528         3176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_730159, whole genome shotgun sequence.
ACCESSION   DASZYN010000528 DASZYN010000000
VERSION     DASZYN010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730159"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1249
                     /locus_tag="VHE33_13525"
     CDS             65..1249
                     /locus_tag="VHE33_13525"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HVT98518.1"
                     /translation="MPPTQHKDYYATLGVKKSATSEDIRKAFRKLARKYHPDVNPGDK
                     KAEEKFKEISEANDILSDDKKRKIYDQFGFYSDQIDPATAEAAARGGYGPGGSRGGAH
                     EGVPFDFGGFDFSEEAPGASTGGFGNFRDIFSGMFSRGGAQAHGPQPGTDLEYTVNVD
                     FWTAIRGGVARLEIQRQEACATCHGRGSMGGTQTCPECHGTGQVTQMGGRMKFNIQCP
                     RCNGSGKVQNQCPTCHGEGVLYRTEPLEFRIKAGTRDGQRIRLAGKGNAGTHGGAAGD
                     LYLVIRAGSHPVFTRTGDDIYLTLPVTVSEAALGARIEVPTIDGRAQLRIPPGTQSGQ
                     KLRMRERGVPSATKDGVRGDEIVTVEVSVPQLRDERSREILREFAKLNPEDPREGIWA
                     KV"
     gene            1397..1894
                     /locus_tag="VHE33_13530"
     CDS             1397..1894
                     /locus_tag="VHE33_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98519.1"
                     /translation="MPLFRGPWPKPPEEQDPDTRRATQMLNAAMSYARLILKRYGQLA
                     PFAFAMDRDGNVARETIEIPRLPRDPERLFKLMTEHVAERVRRGLIQGVALCANVSLA
                     TPSQEGYTDAVDLTIEQTSGHAVQVTVPYKIYGGQMHNLLPRRIALGKLISEEVRPHL
                     FTHAR"
     gene            1948..2361
                     /locus_tag="VHE33_13535"
     CDS             1948..2361
                     /locus_tag="VHE33_13535"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVT98520.1"
                     /translation="MPSKRKSKGAYMISAVAEMYEIHPQTLRLYEREGLLKPSRTEGN
                     TRLYTDEDLERLEFILNLARDLGVNIAGIAIILQMRERMEAMNLQMQDFVNYVRTEML
                     SRMQHAAEPSAAMVPLRRPVIVPSDKPANNRKDRA"
     gene            complement(2451..2714)
                     /locus_tag="VHE33_13540"
     CDS             complement(2451..2714)
                     /locus_tag="VHE33_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98521.1"
                     /translation="MQEDLQMLDEWIPEQMEPGTLFVLENAAPMGDARNPFVAVLACP
                     KCGVLGLITKAQYYGLESMICGGDACSAEYYLREDEIEYRKPH"
BASE COUNT          639 a          919 c         1034 g          584 t
ORIGIN      
        1 gcgccgcgcg atgccaaaac cggagaactg aagccggcaa cggcctgaat cgaaaacgag
       61 aagaatgcca ccaactcagc acaaagacta ttacgcgacc ctaggggtga agaagtctgc
      121 cacctccgag gacattcgca aagcgttccg taagctcgcg cggaaatatc acccggatgt
      181 gaatccgggc gacaagaagg ccgaggaaaa gttcaaggaa atctccgaag ccaacgacat
      241 cctctccgac gacaagaagc ggaagatcta cgaccagttc ggcttctatt ccgatcagat
      301 cgatccggcg accgctgaag ctgcggcgcg cggcggatac ggtccgggcg gttcgcgcgg
      361 tggcgcccat gaaggagtcc ccttcgattt cggcggattc gacttttccg aggaagcgcc
      421 cggcgcatcg acggggggat ttggaaactt ccgcgatatc ttctccggga tgttctcgcg
      481 gggcggggcc caggcgcacg gtccgcagcc gggcaccgat ctggagtaca cggtcaacgt
      541 cgatttctgg acggcgatcc ggggcggtgt ggcgcgcctg gagattcagc gccaggaagc
      601 ttgcgcgacc tgccacgggc gcgggtcgat gggcggcacg cagacctgcc cggaatgcca
      661 cggcaccgga caggtgacgc agatgggcgg acggatgaag ttcaacatcc agtgcccgcg
      721 ctgcaacgga agcggcaagg ttcagaatca gtgtccgacc tgccatgggg aaggcgtact
      781 ctatcgcacc gagccgctgg aattccgcat caaggccggc acccgggacg gacagcgcat
      841 ccggctcgcc ggcaagggca atgcgggcac gcacggcggt gcggcgggcg atctttacct
      901 ggtgatccgc gcgggatcgc atccggtatt cacgcgaaca ggcgacgata tttatctgac
      961 cctgccggtg acggtgagcg aagcggcgct gggcgcgcgc atcgaggtgc ccacgatcga
     1021 cggacgcgcg cagctgcgga ttcccccggg aacccagagc ggacagaagc taaggatgcg
     1081 ggagcgcggc gtgccgtcgg ccacgaaaga cggcgtgcgc ggcgacgaga ttgtgaccgt
     1141 cgaggtctcg gtgccgcaac tgcgcgatga gcgctcgcgg gaaattctgc gggagtttgc
     1201 gaagctgaac ccggaggatc cacgcgaggg aatctgggcg aaggtttagt tgagccgctt
     1261 tccgggcgtg agtagcccgc aggcgaaatc ctgagcgagc ggagcgagtc gaaggatctc
     1321 tgaaatgagc attgatttca gtaacgatga gattgggtgt tttgcggtag gtcactttag
     1381 aaaggttgca gtgtagatgc cactgtttcg cggaccatgg ccgaagcctc ccgaggagca
     1441 ggatccggac acgcgacgcg ccacgcagat gctgaacgcg gccatgtcgt atgcgcggct
     1501 gatcctgaag cgctacggcc agctggcgcc atttgcgttc gcgatggacc gcgacggcaa
     1561 cgtggcgcgg gagacgatcg agattccgcg gctcccacgc gatccggagc gcctctttaa
     1621 gctgatgacg gagcatgttg cggagcgcgt ccggcgggga ttgatccagg gagtggctct
     1681 gtgcgccaat gtcagtttgg ccacgccttc gcaggagggc tacaccgacg ccgtcgatct
     1741 gaccatcgag cagacgtcgg gacatgcggt gcaggtaacg gtgccgtaca aaatttatgg
     1801 cggacagatg cacaatctgc tgccgcgacg gattgcgctg ggcaagctga tttccgagga
     1861 ggttcgcccg catttgttta cgcacgcgcg atgagggaac aattcgctcg cccatgccgg
     1921 gcgcgcagtg cataatgtga atcggccatg cccagcaaac gcaaatccaa aggcgcgtac
     1981 atgatctcgg cggtcgccga gatgtacgag atccatccgc agaccctgcg gctctacgag
     2041 cgtgaggggc tgctgaagcc gtcgcgcacc gagggcaaca cgcgactgta cacggatgag
     2101 gatctggagc ggctggagtt catcctgaat ctggcgcgcg atctgggggt gaacatcgcc
     2161 gggatcgcga tcatcctgca gatgcgggag cggatggagg cgatgaatct ccagatgcag
     2221 gattttgtga actacgtgcg cacggaaatg ctgtcccgca tgcagcacgc tgccgagccc
     2281 agtgccgcaa tggtgccgct gcggcggccg gtaattgtgc cctcggataa accagcgaac
     2341 aaccggaaag acagagctta gaaattcgag ttgagagctg ctcgggcgtt gctctctgcg
     2401 ccaaaacaga aaagcgtgcc cgaaggcacg cttttcttct atcccctgct ttagtgaggt
     2461 ttccggtatt cgatctcgtc ttcgcggagg tagtactccg ccgagcaggc atcgccgccg
     2521 cagatcatgg actcgaggcc gtagtactgg gctttcgtga tcagtcccag cactccgcat
     2581 ttcgggcacg ccagcaccgc gacaaaagga ttccgcgcgt cacccatcgg cgccgcattc
     2641 tccagcacaa agagcgttcc tggttccatc tgctccggta tccactcgtc cagcatctga
     2701 agatcttcct gcatataacc tccgtgtgtt gtgctgtgtg ctgttgcatc cggacttcgt
     2761 gcgacgccgg atcgaattgc gggtgctcat cgagtggtga ttttggcgct gcctcctctc
     2821 gcggaaccgc ggctccgccc gtaacaggaa actcctgttt cgttagaccc tgattgtcgg
     2881 gaaatatcgc tctggccgaa ggatgggatt cggctcgcgc cgtcagcacc gcgaatcgcg
     2941 cggttggcct tgctcgtgct cgattctcgc tcgggcatca caggcggcgt ctctgggtcg
     3001 cctcgatccg gtacgatgca agatcgcgaa tcctggcagc ccgtcagttg acgccattct
     3061 cagtgcgacg ccatgaagtg tcaatgccaa ctttggggcc aggttatctc accaggttcg
     3121 ggcgctgtgt aacgcgtcct ggtcacaagc gcgacgatca cttttgtgtc atgcgg
//
LOCUS       DASZYN010000529        16526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_668411, whole genome shotgun sequence.
ACCESSION   DASZYN010000529 DASZYN010000000
VERSION     DASZYN010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 16526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16526
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668411"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VHE33_13545"
     CDS             complement(<1..995)
                     /locus_tag="VHE33_13545"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HVT98522.1"
                     /translation="MRLAVLAFVMGASLCFAQEQSDFKPATSNVLDAQYPKVDSKSRV
                     QIRFKAPDATQVRVNFWSGLKAPMEKQPDGYWTIITPPMAPGLHYYTVVVDGAEVSDP
                     GSTAYFGGSKWASAVEVPEAGADYYQPQDVPHGQVREVWYHSGVTGTWRHALVYLPPN
                     YDTAKARYPVLYLQHGGGEDETGWIRQGRANFILDNLIASGNTKPMIIVMANGYASRA
                     GYVVPDLTGQPFGSPEFMKVMQERMGAFEDDMTQALIPFIDKTFRTIPDRDHRAMAGL
                     SMGGMQTFQVTFDHLDQFSYIGGFSGAANPFLMGADKLDTKTAFHGAMADPSAFA"
     gene            complement(1137..2912)
                     /locus_tag="VHE33_13550"
     CDS             complement(1137..2912)
                     /locus_tag="VHE33_13550"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HVT98523.1"
                     /translation="MASSGPHRAQFDSRHRPITAGGFVKTGPLVFRDVAAVAGLTKWR
                     HQAGTPEKQLILEEKGPGVCLLDYDNDGWLDIYMVNGSTYAALAGNAPPPHAALFHNN
                     HNGTFTEVAGKAGVVNDRWGYGCAVGDYDNDGWPDLYVTNFGKNRLYHNNGDGTFTDV
                     AANAGVTLGTWSTGATFGDYDGDGRLDLFVAGYAQIDLADPPLYGKKGIDNALCEYYR
                     LPVMCGPRGLHGEHDHLLHNNGDGTFTDVGQKLGVDDPRGYYGLGALFADVNNDGRPD
                     LLVANDSTPNYLYLNKGDGTFENQSEVSGYAFNGDGREVANMGIAVGDYENKGRLSVV
                     NTDFANDYNVLFQNDGTGSFLDVSYHAGIADSSIPFVGFGDGFLDFDNDGWKDLLIVN
                     GHVYPQVDQHPEWGQSYAQRPLLYRNLGNGTFALVPAVEGTGLAKLMVGRGAAFGDIF
                     NDGRISVVVNNMDGVPVLLRNVSSDHHHWVELRLIGGSKSPRDAVGATVYLNAAGVRQ
                     RGDVLSGGSYLSSNDLRVHFGLASATDAGTAEIHWPSGAKEAVKLPAVDKIYTVTEGK
                     GITATLCTAPFCETKQIVGKAQFIH"
     gene            complement(3089..4732)
                     /locus_tag="VHE33_13555"
     CDS             complement(3089..4732)
                     /locus_tag="VHE33_13555"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HVT98524.1"
                     /translation="MFFRDIGRQSGLTTAPHSSTDRRYIVETMAGGGIALFDCDNDGK
                     LDIAVVNDSTIDRYRAGGDPMLTLYHQDGPAGRIHFTDITQSAGLNTRGWGMAIAVGD
                     YDNDGLPDLYVTGYGHNVLYRNQGRCRFKDVTERAGVALSGFSTGAAWADYDRDGFID
                     LFVSRYVKEDIQHLPSLVPPLKANETDMLEVPDAMAGESDVLLRNRGDGTFEDVSAKA
                     TVDNTAKLHGMGVIWGDYDNDGWPDLFVANDGGANYLYRNLGNGTFEENGVSTGTALG
                     PNGETYGNMGGDFGDFNRDGRLDLVTTRYRRQPVSLYRNDDVIFTDAAAATGLANDTA
                     APVKWGVGFGDFDDDGWPDILIANGNFSSLTDRLPSEVRFREPLQLFRNFEGQTFRDV
                     ASKAGLNAGPLQSRRGIAFGDINNDGKLDFVVFNVNAPPSLFLNERQDENHHVLFHLV
                     GDRSNRMAIGTRVTVATMGMRQIDEVRGGDGYNSCNDSRLHFGLGTASVMSEVEIRWP
                     SGLHQKFANVPADATYEIREGKGLRKVAELNTYSTGGSH"
     gene            complement(4988..6160)
                     /locus_tag="VHE33_13560"
     CDS             complement(4988..6160)
                     /locus_tag="VHE33_13560"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT98525.1"
                     /translation="MRRLFPGFLCALLFAAFSLGAAAQADQGQEEKIQGLEQQVQKYL
                     QEQKPQLAVPVLREILLLDPKNLNANANLGVLLFFQNNYADAIPPMRTALQAKPNLWR
                     IQALAGIAEKRTGDPAAAQTDLEQAFPHLEDIKIKKQAGLELVELDSSAGQLAKAATV
                     TETLQELSPQDPQILFAAWEISSQMMQQSLLNMLLVAPDSAEMHMVMGGQLGRQGDHA
                     GAIAQYRKAIQLNPRLAGVHFELAEQLRASPDPALNAQAEAEYKAAVQENHYDVKALC
                     RLGDSIAARGDFKAAEEDYRKALAVQPKDADARTGLAIALITMNQTDEALPLLEGAVR
                     DDPTNAVAHYRLSVLYRRAGRTAEAQHEMMEFNHYKELKARLGQVFQQVRTQSDQP"
     gene            complement(6320..8023)
                     /locus_tag="VHE33_13565"
     CDS             complement(6320..8023)
                     /locus_tag="VHE33_13565"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HVT98526.1"
                     /translation="MTKGKSGSGILLNRRQALLLSATAGMGAALPKTSFASDSTKTAA
                     HQEPGNCSTPRSAVANTQYGKVRGFLDGGVLTFKGVPYGQTTAAENRWLPAKPPTPWK
                     DEYPALMYGGNCPQRLHDWTGIEQCFIQDWDDGYQSEDMLKLNIWTPALTGSRPVMFY
                     IHGGGYSFGSSYELPSHEGAQMARHHDVVQVSVNHRLNILGFFDVSDIGGSGYEDSVN
                     VGMTDLVAALRWVHENIANFGGDPDKVMIYGQSGGGSKVTTLMGMPSASGLFHRAAAQ
                     SGGGGNAPSKEQQKEVARLVMKDLGLQPNDIASLQKMEWHQLVAAGDAAISKINPQGR
                     GMLGPFAPGPPRVGWSPCVDDKIINLRSFHDVAPEISKNVPMLIGSVSEEGNHMLSRP
                     TEEEWHANLAKVYGDDKATAIVATLKKAYPRKKIQTLSYMCSGTFGLNGLSMRNNVVK
                     MAKLKYEQKAAPAYTYYFTWQTPILDGLPGAWHTADLQFCFDNTKRCEQGTGNTPEAQ
                     ALAKKMATAWANFARTGNPGQPGLAWASTDPETNKTMVWDNQCRMENDPEGDARKILL
                     S"
     gene            complement(8230..11529)
                     /locus_tag="VHE33_13570"
     CDS             complement(8230..11529)
                     /locus_tag="VHE33_13570"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT98527.1"
                     /translation="MQSLGLLFALSTGMAFAQATNSADITGTVTDATGAVIPGVTVTV
                     TDVDKNVEHTYTTNAAGLYDTGPIVPEDNYTIVFKKDGFSSLQRGPMLLGIGRTGLNV
                     QLNVGQTTQRVVVNESAPLLQTTSAEMSQTLPEQTLTTLPQTGTPDWQSFVVLQPGVS
                     GAPQNGNSASNPGAGGAAANGSMPFSTAMLDGSSVSSPMSDNVINTPILDTVGEVKMS
                     DSNFSAQYGTGGIIFNQISKGGTNQFHGLAYDYFQNTALNAASYGFGFGKVPAIHWND
                     FGFQVSGPVIKNRIFFLFDWDHTINHGGASVTTTTVPTAAMKMGDFTGLATIYDPTTQ
                     AVAGTTVTRQSFASEYGNGNVIPAGMIDPVAKNIQALFPSPTLPGTVNNFQYLTAPKS
                     TLQKYFGRFDADITKNNHLSGSSAYNYTLNTGLSPVCPVGCFDIDVENMSGQITDVHT
                     FSGRTINEAKAGWMGEYDLLDPQTRGAGWPAKLGLQFAKQDIFPTINISGGVYSLAPG
                     THANYKENVFNESDVVTMIRGRHSLHFGGEMIAYRADSTAWGNINAATLGFTGVYTQQ
                     GNTTGTTTGGSPYADFLLGYANSWSAGFSPEYGGRLKNPGIFAQDDWKLTPKLTVNLG
                     LRWEGRTGWSDSTKNERTFDPSITNPATNTSGAMWYALTHMNGRTALQKSTWNNWLPR
                     AGFAWEINQKTTLRGGFGLYTFPWNVDNYASSGLGNARSQSGSEKDSTTNINPVVLLS
                     SDGNTNYQGAKGASINSLYVTAPTTPQAYNGQGVSYIPYDQSLPLLKNWNLTAQRQIS
                     GNTMIEVAYVGSRGTNLLFNTDLNQVPVSKLGPGDSIYRPYPQYQTINGFTTQGYSNY
                     HALQAQYVRRMSNGLMFNFNYTWSHMLDNQDSSGWGSKEGTTVWQNAHDPDANYGAAN
                     FDIRHMFKAYGSYDLPFGHGRRYLSSNKALDEAVGGWTLSLTWLSQGGNPFTPVVTGG
                     DNSYSQSGGGGYAWFPDQVGDPKSGDFKGIHGWYDTSAYRAPAAGTLGNMRRNSIYGP
                     GVYVMNGAVHKSFPIRERVSFDFAANATNVLNHPSFAQPDINIGPGHSGQITGTTVGG
                     RVLMLVGKLRF"
     gene            11805..12692
                     /locus_tag="VHE33_13575"
     CDS             11805..12692
                     /locus_tag="VHE33_13575"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVT98528.1"
                     /translation="MNTTIDQWEVFQTVVQLGSFAAAAKKTNRSQSTISYAISRLQEQ
                     FKVPLFEMKGRKAQLTEAGKALLADAEPVLNGFRALEAKAASLASNEEREIKVSVDSL
                     YPDDRLFRALAHLNRTYPLVRPTVEKKPFLSSAYELETAGADLCITGLPAREHFAKAV
                     LDIRIMAVARADHPLLRQNRELARLDLTQHLAVIIEGANLPEPKRQPHAKTQRILVVH
                     SVESAIEAVRSGMCFGWLPVYRIAPWLNAGELSGLRLPLYGERVVRMFLVLKDAESIP
                     REKLLLADLLGANTDAEAI"
     gene            13082..15238
                     /locus_tag="VHE33_13580"
     CDS             13082..15238
                     /locus_tag="VHE33_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98529.1"
                     /translation="MKARIVLFAAAALWTASAVNASSQEAAPAPPEQIKATIDTQQTA
                     EPVSKYEFGMFIEHIGPLIYRSLWSEMLDDRKFYFPITSKEPAPPAGEDAARVRRNAL
                     RRWVPVGPDDGVVMDKDHPFVGDQSPRVTLDASTPHGIQQAGFALIKGKKYVGRIYLR
                     GTPGSRVRVSLIWGSNPGDRQTVAFTSLTDSYRKLPLNFTAGADTAQGTLEIAGTGSG
                     NFHVGTVSLMPADNVEGFRPDTIALLRQLHSGMWRLPGGNFLSDWSWYNGVGDIDKRP
                     PTFDHAWNAMQSNDVGMDEFMTLCRLIGVDPYITVNAGFGDSHSAAQEVEYINGSVNT
                     PMGAWRAKNGHAEPYHVKFWNIGNEPWGTFQLGYTDLKYYVLKHNEFAKAMRQVDPSI
                     TLIASAKMLEPMSLQGTNRSKYVDNMQPVFGTDVDWTGGILKNCWGTFDGIAEHWYES
                     PGRHFDIEKAKSLSPDAPSTDAWVKYDPTTLEFARYAGDVIRRKAEEWEGYQQRFPEM
                     LQKKTFLSIDEYAYFGGSGFGRGVNLKSALAYAMLFNEEMRHTAFLTMTAHTMGTSTL
                     DITPTASTMNTTGLVFKLYGDHFVGSIPVAVSGNSPQPATKNPPYADEPKSRSGSPTY
                     PLDVFAALSPDHKYLTVSVVNATDKEQKLDLDFAGGRIEGAPTLWRMTGRDLDAADHA
                     GEPAQVSIEEVPMHDASSAVTVAPISIDIYRFPVAQ"
     gene            15297..>16526
                     /locus_tag="VHE33_13585"
     CDS             15297..>16526
                     /locus_tag="VHE33_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98530.1"
                     /translation="MKRRIALFSTAVFGCTLAAFSQQGPTGTPAPIVATVNTQETSAP
                     ISKYLYGGFIEEGGTLISRSLWSEMLDDRKFYFPINSQVTEEPAGQRRRPSFLEPPRK
                     WRPVGPDEAVVMDADAPFVGDHSPRIALDSSTSHGIQQSGLTLVKDKKYIGRIYLRGT
                     SGATVKVSLIWGEDPKDRQTVSFASLTDSYRKFALSFTAGASTNKATFEIAGTGQGNF
                     HVGTVSLMPADNVSGFRPDTIALLRQLHPGMWRLPGGNVISDWVWYDSVGDIDKRPPM
                     FDYAWHAMQTNDVGLDELMTLCRLIDVDPYITVNAGFGDSHSAAQEVEYINGSASTPM
                     GAWRARNGHPEPWHVKYWNIGNEPYGSWQLGHTDLKYFVLKHNEFAKAMREADPSITL
                     LASGAFPDEMTVEGQARL"
BASE COUNT         3352 a         4709 c         4851 g         3614 t
ORIGIN      
        1 ttcgcgaagg ctgacggatc ggccatcgcg ccgtgaaacg ccgtcttcgt atccagcttg
       61 tcggccccca tcaggaaagg attggccgct ccgctgaatc caccaatgta cgaaaactgg
      121 tcgagatgat cgaacgtcac ctggaaagtc tgcattccgc ccatggaaag tcctgccatg
      181 gcgcggtggt cgcgatccgg gatcgtccga aaggttttgt cgatgaaagg aatcagcgcc
      241 tgggtcatgt cgtcttcgaa ggcacccatc cgttcctgca tcaccttcat gaattccggg
      301 gatccgaatg gctgccctgt caggtcggga actacataac cggcgcggct cgcgtagccg
      361 ttcgccatca caatgatcat cggcttcgtg ttgccgctcg cgatgagatt gtcgaggata
      421 aagttcgccc tgccctggcg gatccacccg gtctcgtcct cgccgccgcc gtgctgcagg
      481 taaagcaccg ggtagcgagc tttcgccgtg tcgtagttcg gtggcagata caccagcgca
      541 tgccgccacg ttcctgtgac gcctgagtga taccagacct cgcgcacctg cccgtgggga
      601 acatcctgcg gctggtaata gtcggctccg gcctcaggga cctccacggc gctggcccac
      661 ttgctgccgc cgaagtacgc cgtgcttccg ggatcgctca cctcggctcc gtcgacgacc
      721 actgtgtagt agtgcaatcc cggggccatg ggcggcgtga tgatagtcca gtagccgtct
      781 ggctgttttt ccatcggcgc ctttagccca ctccagaagt tgaccctgac ctgggtcgcg
      841 tcaggcgcct tgaagcgaat ctggacgcgt gacttgctgt cgactttggg atattgcgcg
      901 tcgagaacgt tggaggttgc aggcttgaaa tcgctttgtt cctgcgcaaa acacaggctc
      961 gcgcccatga caaatgccag cacagccagt ctcatcagtt ccacctatat attgcgattc
     1021 gttggtctgg tcaccggccg gaatgctgca gggccgggcg cgaacgttga ccacgcgcat
     1081 gttaattcac gccatcgcac ggcgatagtc gcattttttg cgaataaaag atcgatttaa
     1141 tgaatgaatt gggctttgcc aacgatctgc ttcgtttcgc agaagggggc ggtgcagaga
     1201 gtggcggtga tcccctttcc ttccgtgacg gtatagattt tgtcgactgc gggaagctta
     1261 accgcttctt tcgctccgga gggccagtga atttctgcgg tgccggcgtc ggtcgcactc
     1321 gccaacccga agtggacgcg caggtcgttc gatgagagat agctgcctcc gctcaggaca
     1381 tcgccgcgct ggcgaactcc tgcagcattc agatagacgg tggcgccgac cgcgtcgcgg
     1441 gggctcttcg atcctccgat cagcctcaac tcgacccagt gatgatgatc ggagctcacg
     1501 ttgcgcagga gcaccgggac gccgtccata ttgttcacga caacactgat cctgccatcg
     1561 ttgaagatat cgccgaatgc tgcgccacgg cccaccatca acttcgcgag tccggttcct
     1621 tctacggcgg gaacgagcgc gaaggttccg tttccgagat tgcgatagag gagcggccgc
     1681 tgggcatagc tttgtcccca ctccggatgc tggtccacct gcggatagac atgcccattg
     1741 acgatgagca ggtctttcca gccgtcgttg tcgaagtcga gaaacccatc gccgaagccg
     1801 acaaagggga tggaggaatc ggcgatgccg gcatggtaac tgacatcgag gaaagagccg
     1861 gttccgtcgt tctggaagag aacgttatag tcgttcgcaa aatcggtatt cacgacggac
     1921 aggcgcccct tattttcgta gtcgccgacg gcgatgccca tgttcgcgac ttcgcgtccg
     1981 tcgccgttaa acgcatagcc actgacttca ctctgatttt cgaacgtgcc atcacccttg
     2041 ttgaggtaaa ggtagttcgg cgtggagtca ttggcgacca gaagatccgg cctgccgtcg
     2101 ttgttgacat cggcaaagag cgctcccagg ccgtaatagc ccctcggatc atcaacgccc
     2161 agcttttggc ccacgtcggt aaaggttccg tcgccattgt tgtggaggag gtggtcatgc
     2221 tcgccgtgca aaccccgtgg gccgcacatc acgggaagtc tgtaatactc gcagagcgca
     2281 ttgtcgatgc ccttctttcc ataaaggggc gggtcggcca gatcgatttg cgcgtagccg
     2341 gcaacgaaca ggtcgaggcg gccgtcgcca tcgtagtcgc cgaaagttgc acccgtagac
     2401 catgttccca gcgtcacacc ggcgtttgcc gcaacgtcgg taaaggttcc gtcgccattg
     2461 ttatggtaga ggcggttctt tccaaagttg gtcacataga ggtccggcca gccgtcgttg
     2521 tcgtagtctc cgaccgcaca accatatccc cagcgatcgt tgactactcc ggcttttccg
     2581 gctacctcgg taaaggttcc gttgtgattg ttgtggaaga gcgctgcatg cggcggaggg
     2641 gcgttgcctg ccagtgcggc ataagtcgat ccattgacca tatagatgtc cagccacccg
     2701 tcattgtcgt agtcgagcag gcagacgccc gggcctttct cttcgaggat cagctgcttt
     2761 tccggagttc ccgcctggtg gcgccacttt gtaagtccgg ctacggcggc gacgtccctg
     2821 aaaaccagcg gacctgtctt cacaaaaccg cccgcagtta tggggcgatg ccggctgtcg
     2881 aactgagctc gatgcgggcc gctgcttgcc attcctgtct gcggtcggcg tccaggcgct
     2941 ggggaaggcg tcgtgccgga atcctgcgca accagcagga ggggcgcagc cgacgcaaga
     3001 agcaaaacaa cgatctttgc gacgagtctc ccttgtatgt gctgcgcccg agcacatacg
     3061 aggcgactcc cggtgctgct tttcgggttc agtggctgcc gcctgtactg taagtgttaa
     3121 gttcagcaac tttgcgaagc cctttgccct cccggatctc ataagtggcg tccgccggaa
     3181 cattcgcaaa tttctggtga aggccgctgg gccagcggat ttccacctcc gacataacgg
     3241 atgccgtgcc taatccaaag tgcaggcggg aatcgttgca ggaattgtag ccgtccccgc
     3301 cgcggacttc gtcaatctgc ctcataccca tcgttgcgac agtcactcgg gttccgattg
     3361 ccatgcggtt acttctgtct cccaccaggt ggaagaggac gtggtggttt tcatcctggc
     3421 gttcattcag aaacagggat ggcggcgcgt tgacattgaa gacgacgaaa tcgagtttgc
     3481 cgtcgttatt gatatctccg aatgcgatcc cccggcgcga ttgaaggggg cctgcattca
     3541 gaccggcctt gctcgcaacg tcacgaaaag tctgaccctc gaagttgcga aaaagctgaa
     3601 gaggctcgcg gaaccgaact tccgatggca accgatccgt gagagaagag aagtttccat
     3661 tggcaatcaa gatatccggc cagccgtcgt catcgaagtc gccgaagccg acgccccatt
     3721 tcaccggcgc ggccgtgtcg ttcgccagtc cggtggcggc ggcggcgtcg gtgaagatca
     3781 cgtcatcatt gcggtaaaga ctcacgggct ggcgacgata acgggttgtc acaagatcga
     3841 gccggccatc tcgattgaag tctccgaaat cgcctcccat gtttccgtag gtttcgccgt
     3901 ttgggccgag tgctgttcca gtgctgacgc cgttctcttc aaacgtgccg ttgccaagat
     3961 tgcgataaag gtagttggct ccgccgtcgt tggcgacaaa cagatctggc caaccatcgt
     4021 tgtcgtaatc tccccaaatc acgcccatgc catgaagctt cgccgtgttg tccaccgtcg
     4081 cttttgccga aacgtcctca aacgtgccgt cgccgcggtt ccgcagaaga acgtccgatt
     4141 ctccggccat cgcatccggt acttcgagca tgtccgtttc attcgccttc aaagggggta
     4201 cgagagatgg caggtgttga atatcttcct ttacatatcg ggagacgaag agatcgatga
     4261 agccgtcgcg atcataatcg gcccacgcag caccggtact gaagccgctc agtgccactc
     4321 ccgcgcgctc ggtcacatct ttgaaacggc accgaccctg attgcggtaa agaacattgt
     4381 gtccgtagcc cgtcacatac agatcgggca gcccgtcgtt gtcgtaatcg ccgacggcga
     4441 tcgccattcc ccagccgcgc gtgttcaacc ctgcggattg tgtaatgtcg gtgaagtgga
     4501 ttcggcctgc gggaccgtcc tggtggtaga gagtgagcat tggatcgccg ccagcccggt
     4561 aacgatcgat cgtgctgtcg ttgacaacag cgatgtccag cttgccgtcg ttgtcacaat
     4621 caaacagggc aatgccaccg cccgccatgg tttcgacgat gtagcgtctg tccgtcgatg
     4681 aatggggagc ggttgtcagt ccggactgac gtccgatatc gcggaagaac atagcaggag
     4741 tctttttcgg gccggaagag ttcgccgtag cgaccgtgct gacggcccga acgggagatg
     4801 gcaaatgcag gcagctctgg cttgacgctg cgaacacgca tgcaagaaac gccgcaccgg
     4861 agcatacagc tgtgattgtg tcggggcgct tcgttattcc tcctgactta ctcggggaaa
     4921 tatggtggaa cttattcgct catttaggaa gcacagttcg acccaacccg aacggcagtt
     4981 ctgcaactta tggctgatcc gattgcgtgc ggacctgctg aaatacctgg cccagcctgg
     5041 ctttaagctc tttgtagtgg ttgaactcca tcatctcgtg ctgagcctcc gcggtccggc
     5101 cggcgcgacg ataaagaacg ctcagccggt agtgggcgac agcattcgtg ggatcatccc
     5161 tgacggcgcc ttccagcagg ggaagagcct catccgtttg gttcatcgtg atcagcgcga
     5221 tcgccagccc ggttcgggca tctgcatcct ttggttgcac cgcgagcgct tttcggtaat
     5281 cctcctctgc ggctttgaag tcaccccgcg ccgcaatcga gtctcccagc cggcacaagg
     5341 ccttcacgtc atagtgattc tcctgaactg cagctttgta ttcagcttcc gcctgcgcat
     5401 tcagcgccgg atcaggagaa gccctgagtt gctccgccag ttcaaaatga acccccgcca
     5461 acctgggatt cagctgaatt gcttttcgat attgtgcgat ggcaccggca tgatccccct
     5521 ggcgcccaag ctggccaccc atgaccatat gcatttcggc cgaatccgga gcaaccagca
     5581 gcatgttcag caaactctgc tgcatcatct gggaagaaat ttcccaggct gcaaaaagga
     5641 tctgaggatc ctgcggcgac aattcctgca acgtctctgt gacggttgct gccttcgcca
     5701 gctgacccgc ggatgaatcg agctccacta attcaagtcc ggcctgcttt tttatcttga
     5761 tgtcttcaag gtgaggaaat gcctgctcga gatcggtctg tgcggccgcc ggatccccgg
     5821 tccgcttctc ggcaattccc gccagcgcct gaatcctcca caggttgggc ttcgcctgca
     5881 gggcggtgcg catgggtgga atcgcatccg cgtagttgtt ctgaaagaaa agcagtaccc
     5941 caagatttgc attggcattg aggttcttcg gatcgagcaa caggatttca cgcagcaccg
     6001 gtacagcgag ctgcggtttc tgctcctgca gatacttctg tacctgttgc tcgagcccct
     6061 ggatcttttc ttcctggccc tgatccgcct gcgctgcggc ccccagcgaa aacgccgcga
     6121 acagcagcgc gcagagaaat cccgggaaaa gccttctcat gcactcactc ttatcacatc
     6181 gaattgagcc aggaaacacg agcagccccg tgccacgatt ccgcggcacg gaacatccag
     6241 gatttgttct gacgccaaac tccaggggcg caaacggctt tttgtcacct cctctgtagt
     6301 gaacaagcaa attcaaaatt cagctcaaca gaatcttgcg tgcgtctccc tcagggtcat
     6361 tctccatgcg gcattggtta tcccagacca tggtcttgtt ggtttccgga tccgtcgacg
     6421 cccaggcaag accgggctgg cccggatttc ccgtccgcgc gaagttcgcc catgccgttg
     6481 ccatcttttt ggccagcgcc tgagcttcag gagtgtttcc ggtgccctgt tcacaacgtt
     6541 tggtgttgtc gaagcagaac tggagatcgg ctgtgtgcca ggcgcctgga agcccgtcaa
     6601 gaatcggcgt ctgccacgta aagtaataag tgtaagccgg cgctgctttc tgttcatatt
     6661 tcaactttgc catcttcacc acgttgtttc gcatggagag gccatttaag ccgaaggtgc
     6721 cgctgcacat atacgagagt gtctgaatct tcttccgggg ataagccttc ttcagggttg
     6781 ccacaatcgc agtggccttg tcgtcgccat atacctttgc gaggttggca tgccactctt
     6841 cctcggtcgg ccgggaaagc atgtggttcc cctcttcgct cacggaaccg atcagcatgg
     6901 gaacattctt tgatatttcc ggagcgacat cgtggaagga tctaaggttg atgattttgt
     6961 cgtcgacgca gggagaccat cccacgcgcg gggggccggg agcaaacgga ccgagcatgc
     7021 cacgcccctg gggattgatc ttcgagatcg ctgcgtcccc tgcggccacg agctgatgcc
     7081 attccatctt ctggagcgag gctatgtcat ttggctgcag cccaaggtcc ttcattacca
     7141 gccgcgctac ttccttttgc tgctctttgc tcggagcgtt tccgcccccg cccgactgcg
     7201 ccgcggcacg atgaaagagt cctgatgccg aaggcatgcc catcagggtg gtcacctttg
     7261 aaccgccgcc agactggccg tagatcatca ctttgtcggg atcgcccccg aagttcgcga
     7321 tgttctcgtg aacccagcgc agcgccgcga ccagatccgt catcccgacg ttgacagagt
     7381 cctcgtaccc ggagccaccg atgtcagaaa catcgaaaaa gccgaggata ttgaggcggt
     7441 gattcaccga aacctgaacc acgtcgtggt gccgtgccat ctgtgcgcct tcgtgcgagg
     7501 gcaattcata agacgatccg aaactgtatc cgccgccgtg gatgtagaac atgaccggtc
     7561 gacttccggt gagggccggg gtccagatat tcagcttgag catgtcttcg ctctgatatc
     7621 cgtcgtccca gtcctgaatg aagcattgct caattccggt ccagtcgtgg aggcgttgcg
     7681 ggcaatttcc gccatacatc agcgcaggat attcatcttt ccatggcgtc ggaggcttag
     7741 cgggcagcca tcgattctcc gcggcggtgg tctggccgta gggaacgccc ttgaacgtga
     7801 ggactcctcc atcgagaaat ccgcggactt tcccgtattg agtgttggca accgcagagc
     7861 gaggggttga gcagttaccc ggttcctggt gcgccgccgt cttcgtgcta tcggaggcga
     7921 aactggtttt cggcagtgca gcgcccatgc cagcggtcgc tgacagcagc agcgcctgac
     7981 gtcgattcag caggattccg cttccggatt ttcctttcgt cattactctc ctttagctgg
     8041 cccgcgcaat ggttttgcgc aggcgcatgt tgctggttcc gggatcttcc catggccaat
     8101 acgttttctc ggggacgaca tcggattcac gttgcaccga tgaagcgatg acgccgccat
     8161 ccacggccgc gcagattcca ccgcggaggc ggaatctgca cattggcagt gaatggatta
     8221 ttgcaaagct cagaacctga gcttcccgac gagcatgagg acgcgaccgc cgacggttgt
     8281 gccagtaatc tgcccactgt gtcctggtcc aatgttgata tcaggctggg caaacgacgg
     8341 gtggttgagg acattcgtcg cgttcgcagc gaaatcgaaa gagacgcgct cgcgaatcgg
     8401 aaaactcttg tgaaccgcgc cattcatgac gtacacgccg ggtccgtaga tactgttgcg
     8461 gcgcatattc cccagcgttc cggcggcagg cgcacgatag gcactcgtgt cgtaccaccc
     8521 atgaatgccc ttaaagtcgc cggacttcgg atcgccgacc tggtcgggga accacgcgta
     8581 acctccgccg ccactctgac tgtagctgtt gtcgccgcct gtgacgacag gggtgaatgg
     8641 attccctccc tgcgaaagcc aggtaagaga gagcgtccag ccgccgaccg cctcatccag
     8701 ggctttgttc gagctcaggt atctccggcc gtggccaaag ggcagatcat aggacccgta
     8761 cgccttgaac atatggcgaa tgtcaaagtt cgcggcgccg tagttagcgt cgggatcgtg
     8821 ggcgttctgc cataccgtag tcccttcctt ggagccccag cccgaggaat cctggttgtc
     8881 gagcatgtgt gaccaggtgt agttgaagtt gaacatcagt ccattgctca tgcgtcgcac
     8941 atactgtgcc tgcagcgcat gatagttcga ataaccctga gtcgtgaacc cgttgatggt
     9001 ctgatactgc gggtagggac gatagatgga gtccccgggg cccagcttgc tcaccggaac
     9061 ctgattcagg tcagtattga agaggagatt ggtgcctcga ctgccgacat aggcgacctc
     9121 aatcatggta ttgcccgata tctggcgctg agcagtcaga ttccagttct tgagcaaggg
     9181 caaggattgg tcatatggaa tataggagac tccttgtccg ttgtacgcct gtggagtggt
     9241 aggagcggtc acatacaggg agttgatgct ggcgcctttc gccccctgat agttggtgtt
     9301 tccgtccgag gagaggagaa cgaccggatt gatattggtg gtggagtctt tctcactacc
     9361 gctttggctt cgcgcatttc ccagtccgga gctggcgtag ttgtcgacat tccacgggaa
     9421 ggtgtacaag ccaaagccgc cgcgcaacgt tgtcttctgg ttgatctccc aggcaaaacc
     9481 ggctcttggc agccagttgt tccaagtcga tttctgtaac gcagtccttc cgttcatatg
     9541 cgtcagggca taccacatag cgcctgacgt gtttgtggcc ggattggtaa tggagggatc
     9601 gaaagtccgc tcgttcttcg tactgtcaga ccaacctgtt cttccctccc agcggaggcc
     9661 gagattgacg gtcagtttcg gtgtgagctt ccagtcatcc tgagcgaaga tgccggggtt
     9721 cttcagtctg cctccatact cgggtgagaa tccggcggac caggagttgg cataaccgag
     9781 gaggaaatct gcataaggag agcctcctgt ggtggttcca gtggtattgc cctgctgggt
     9841 gtatacgccg gtgaaaccga gtgtcgctgc gttgatgttg ccccacgcgg ttgaatcggc
     9901 gcggtaggcg atcatttcgc ccccgaagtg gagggagtgg cggccgcgaa tcatcgtcac
     9961 tacgtccgat tcattaaaga cgttttcttt gtagttggca tgggttccag gcgccaggga
    10021 atagacgcca ccactgatgt tgatggtggg gaatatatcc tgcttggcga attgcagacc
    10081 cagtttcgcc ggccagcctg cgccccgggt ctgaggatcg agcaggtcat actcacccat
    10141 ccaaccggcc ttggcttcat tgatggtcct gccgctgaag gtatgaacgt cggtaatctg
    10201 cccgctcatg ttctccacgt caatgtcaaa acagcccacc gggcatacgg ggctcagacc
    10261 tgtattgaga gtgtagttat aagcggagga tccggacagg tgattatttt tggttatgtc
    10321 cgcgtcaaat cgaccaaagt acttttgcag agtcgatttg ggcgcggtga ggtactggaa
    10381 gttgttgacc gtacctggca gtgtcgggga cggaaacaga gcctggatgt ttttcgcgac
    10441 cgggtcgatc atgccggcgg gaatgacatt gccgtttccg tattcgctgg caaacgactg
    10501 acgcgtcacg gtcgtgccgg caaccgcctg cgttgttgga tcgtaaatgg tcgcgaggcc
    10561 ggtaaaatcg cccattttca tggcagcggt gggcacggtg gtggttgtga cagaagcgcc
    10621 gccatggttg atggtgtgat cccagtcgaa gagaaagaag atcctgtttt tgattacggg
    10681 gccgctgacc tggaagccga aatcattcca gtggatagcg gggaccttgc caaagccgaa
    10741 cccgtaggaa gcggcattca gggcggtgtt ctggaagtag tcgtaggcga ggccgtgaaa
    10801 ctgattggtg ccgcccttgc tgatctggtt gaaaatgatg cctccggttc cgtactgagc
    10861 agagaagttc gagtcgctca tcttgacttc tccaacggtg tccaggatcg gagtgttaat
    10921 cacgttatcg ctcatcggcg agctgaccga cgagccatcg agcatggccg tggaaaacgg
    10981 catgcttccg ttcgcggcag ctccgccggc gcccgggttc gaggcgctgt tcccgttctg
    11041 cggcgcgccg gaaacacccg gctggagaac aacgaaggac tgccagtccg gcgtgccggt
    11101 ttgaggcagt gtcgtcaggg tttgctcagg gagcgtctgc gacatttccg ccgaagtcgt
    11161 ctggagcagc ggggcagatt cgttgaccac cacccgctgc gtcgtctggc caacgttcag
    11221 ctgaacattc agacccgtcc gaccgatgcc caggagcatg gggccgcgct gaagagacga
    11281 aaagccatct tttttgaaaa cgatggtgta gttgtcttcg ggaacgatcg ggccggtatc
    11341 gtaaagacct gcagcattgg tggtgtacgt gtgttccacg ttcttgtcga catcggtcac
    11401 cgtgaccgtt acgcccggga tgacagctcc ggttgcgtca gtaacggtgc cagtaatgtc
    11461 cgcagagttg gttgcctgtg caaatgccat gccggtggac agtgcaaaca gcaggcccag
    11521 ggattgcatt ctgaagatca tctcgcctcc aagattttcg gtgccgaact gtgaaaagct
    11581 gaagccttct ggggttccgg ttttcagcta cagaggggaa acgtttacct attggcacat
    11641 tttcggagtg atcacttgat catcacgaaa agcaaccggc atgctagttc agtaaaattt
    11701 cagctggcaa gttgtatttt tcgaatggca atttcgaaaa tatcgataat gtgtttcgct
    11761 tttgctggcg cggcgaagga ctgatcgcaa acggagtctt cctgatgaac acgacaatcg
    11821 atcaatggga ggtgttccaa actgtcgtgc agctgggcag ttttgctgct gctgcgaaga
    11881 agacgaaccg ctcccaatcc accatcagct acgccatttc gcgcctgcag gaacaattta
    11941 aggttcctct gtttgagatg aagggccgca aggcgcagct caccgaggcg ggcaaggccc
    12001 tgttggcgga tgcagagccg gtactgaatg gatttcgagc gctggaggca aaagccgctt
    12061 cgctggcgtc gaatgaagag agagaaatca aagtctccgt cgacagcctt taccctgacg
    12121 accggctttt cagggctctt gcccacttga atcggacgta tcccctggtg cggccgacgg
    12181 tggagaagaa accgtttcta tcctcggcct acgagctcga gacggccgga gccgatctgt
    12241 gtatcaccgg acttccggcg cgagaacatt tcgcaaaagc ggtgcttgat atccggatca
    12301 tggctgtggc ccgcgccgat cacccgctgc tccgtcagaa ccgggaactt gcgcgactgg
    12361 atcttacgca gcacctggcc gtgatcatcg aaggcgcaaa cctgccggaa ccgaagcgcc
    12421 agccccacgc gaaaacgcag cggattttag tcgtgcattc agtcgagtcc gcgattgaag
    12481 cggttcgcag tggcatgtgc ttcgggtggc tgcccgtata tcgcattgcg ccgtggctga
    12541 atgcagggga attgtccggg ctccggctgc cactgtacgg ggaacgagtt gtccgaatgt
    12601 tcctggtcct caaagacgcc gagagcatcc cgcgtgaaaa gcttctgctg gctgacctgc
    12661 ttggagcgaa caccgacgct gaagcaatct gaaggaggcg cggacctgat cggaggtttc
    12721 agggtgcgcc aaagcctcga atgtgagcag gccgtcgctg cagcgcagat ccgatcatcg
    12781 aatctatcga ttttgtttct tcgaaacttc ggtcttttca ttgaaatgcc tggctctgta
    12841 ggatgcctgt ggttccgaag cgatcgaggg taatcgtttt cgcgatttcc gctccctggc
    12901 aacagccggt tgttcgaaag ccggttcccc gtcaaacttc tggtcctgga cgaaaacatt
    12961 ggctggcctc aggtttgttt tgttcacaac tcgtccgcaa gaactgcaga tgacagaaaa
    13021 catctcgaca cgggtcgact gcagattcct ccaaagacaa caactcaaca ggagagctcg
    13081 aatgaaggcg agaattgttc tgtttgccgc cgcggcattg tggaccgcga gcgcggtcaa
    13141 cgcttcatct caggaagctg ctccggcgcc tccggaacaa atcaaagcaa cgatcgatac
    13201 gcaacagacg gcagagccag tttcgaaata cgaattcggg atgttcatcg aacatatcgg
    13261 cccgctgatt tatcgcagcc tgtggtcgga gatgctggat gaccggaaat tctatttccc
    13321 gatcacatcc aaagaacccg ctccgccggc cggggaagac gccgcccgag tgcgcaggaa
    13381 tgcactacgc cgctgggtcc cggttggacc ggatgacggg gtggtgatgg ataaagatca
    13441 tccgtttgtc ggcgaccaga gcccgcgcgt gacgctcgat gcatcgacac cgcacggcat
    13501 tcagcaggcg ggattcgcac tgatcaaggg caaaaagtac gtgggacgga tctatcttcg
    13561 cggaactccg ggctcccggg tcagggtcag cctgatctgg ggatcgaacc cgggcgatcg
    13621 ccagaccgtt gcctttacgt cgctgaccga tagctacagg aaattaccgc tcaatttcac
    13681 ggctggcgcc gatacagcgc aggggacgct tgagatcgcg ggtacgggca gcggaaattt
    13741 ccatgttggc acggtgtccc tgatgccggc ggataacgtc gagggctttc ggccggatac
    13801 gatcgctctc ctccgccagc tgcactcggg aatgtggcgc ctgccaggcg ggaatttcct
    13861 gtcggactgg agctggtaca acggcgtcgg cgatatcgac aagcgtccgc cgaccttcga
    13921 ccatgcatgg aatgcgatgc agtcgaacga cgtgggcatg gacgagttca tgacgctgtg
    13981 caggctgatt ggcgtggatc cctacattac ggtcaatgcc ggcttcggcg attcacactc
    14041 cgcggcgcag gaagtggagt acatcaatgg ttccgtcaac acacccatgg gagcgtggcg
    14101 cgcgaaaaac ggccatgccg agccgtacca cgtgaagttc tggaacattg ggaacgagcc
    14161 atggggcaca tttcagctcg gctacaccga cctgaagtat tacgtcctca aacacaacga
    14221 attcgcaaag gccatgcgcc aggtcgatcc ttcgatcacc ctgattgctt ccgcaaaaat
    14281 gctggagcca atgtcgctgc aggggacgaa tcggtccaaa tatgtcgaca atatgcagcc
    14341 cgtgttcgga accgatgtgg actggacggg cggcattctg aagaactgct ggggaacctt
    14401 tgacggcatt gccgagcact ggtacgaatc gcctggcagg cacttcgata ttgaaaaggc
    14461 aaagagcctg tcaccggatg cgccgtccac cgacgcgtgg gtgaagtatg acccgaccac
    14521 tcttgagttc gcgcgatacg ccggtgacgt gattcgccgc aaggccgaag agtgggaggg
    14581 ttatcagcag cggtttccgg agatgctgca aaagaagacg ttcctctcca tcgatgagta
    14641 tgcgtacttc gggggcagcg gctttggccg gggagtcaac ctgaaatcgg cgcttgccta
    14701 cgccatgttg ttcaacgaag agatgcggca cacggccttt ctgaccatga cggcccacac
    14761 catgggcacc tcgacactgg acattactcc gaccgcctca acgatgaata ccaccggcct
    14821 ggtgttcaag ctgtacggcg accactttgt gggctcgatc ccggtggccg tttccggcaa
    14881 ctcacctcag cccgcgacca aaaacccacc ttatgcggat gaaccgaaat cgaggtcggg
    14941 aagtccgacc tacccgctgg acgtgttcgc tgcactcagc ccggatcata aatacctgac
    15001 tgtgtccgtg gtgaacgcca ccgacaaaga acagaagctg gatctggatt tcgccggcgg
    15061 ccggatcgaa ggtgcgccga cgctgtggcg gatgaccggc agggatctgg acgcggctga
    15121 tcatgccgga gagcccgcgc aggtatccat tgaggaagtc ccgatgcatg acgcgtcctc
    15181 tgcagtgacg gtagcgccga tcagcatcga catttaccgg tttccggtcg cgcagtgaga
    15241 atcggccggg tgcctgccgg gcatcgatca tgaatacaag acatcagagg agcgcaatga
    15301 agagacgaat cgcactgttc tcgacggcgg tattcggatg cacccttgcg gcattctcgc
    15361 agcaggggcc aacgggcacg ccggctccga ttgtcgccac cgtgaacaca caggagacct
    15421 cggcgcccat ctccaagtat ctctatggcg ggttcattga ggaaggcggg acgctgatct
    15481 cgcgcagcct gtggtcagag atgctcgacg accgcaaatt ctatttccca atcaactcgc
    15541 aggtcacgga agagccagca ggacagcgcc gccggccctc atttctggag cctccgcgca
    15601 aatggcgtcc tgtgggaccc gatgaagcgg tggtcatgga tgcagacgcg cccttcgttg
    15661 gcgatcacag tcctcgcatt gctctcgact cctcgacttc gcacggcatt cagcagtccg
    15721 gcctcacgct ggtgaaggat aagaagtaca tcggccgaat ctatctgcgc gggacttcgg
    15781 gcgcgacggt caaggtgagt ctgatctggg gtgaggatcc gaaggatcgc cagacggtca
    15841 gcttcgcttc acttaccgac agctacagga agttcgccct cagcttcacg gcgggcgcga
    15901 gcacgaataa ggccacgttc gagattgcag gtaccgggca gggaaatttc cacgtcggca
    15961 ctgtgtccct gatgcccgcc gataacgtca gcggcttccg tcccgacacg attgctcttc
    16021 tgcggcagct tcatccgggc atgtggcgcc tgcccggcgg caatgtcatt tccgactggg
    16081 tctggtacga ctcggtgggc gacatcgaca aacggccccc gatgttcgat tatgcatggc
    16141 acgccatgca gaccaatgac gtgggtctgg atgagctgat gactttgtgc aggctgatcg
    16201 atgtggatcc gtatatcacc gtgaacgcgg gattcggcga ttcccactcc gccgcgcagg
    16261 aagtggagta cattaacggc tcggcgagta cgccgatggg agcctggcgc gcccgcaacg
    16321 gtcaccctga gccgtggcac gtgaagtact ggaacatcgg caatgagcct tacggttcgt
    16381 ggcagctcgg ccacacagac ctgaaatatt tcgttctgaa gcacaacgag ttcgcgaagg
    16441 cgatgcgcga agccgacccg tcgattacgc tgctggcctc gggcgcattt cctgacgaga
    16501 tgaccgtgga aggtcaggcg cggctc
//
LOCUS       DASZYN010000530         3174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_29152, whole genome shotgun sequence.
ACCESSION   DASZYN010000530 DASZYN010000000
VERSION     DASZYN010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29152"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VHE33_13590"
     CDS             <1..1071
                     /locus_tag="VHE33_13590"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVT98531.1"
                     /translation="YLMLATGATHSYFGNDAWEPLAPGLKTIEDAIEIRRRILLAFEL
                     AEREMLETGSHHALNFVIIGGGPTGVELAGAICDITRRYMQRDFRHIDPAKARVLLIE
                     GEKHVLANYPEDLSIKAEEQLKALGVEIHGGMRVTDVQPGYVIAGGQRIESAVTLWAA
                     GVQASPLGKMLGMPTDKRGCVLVNSTLNPEGHPEIFVCGDLAHVEQDGRQVPGVAQPA
                     MQMGDHIAKMIAADLNHQPRPPFHYFDKGDLATIGRLAAVGNVKWPFRGHLSGFPAWI
                     TWLVVHIYFLIGFRNRIAVLSQWIWAYFTFTYGVRLIYGSQALPGWTDENGVDKSPGA
                     HPLDHTTPGSHTEVPSKSAAAD"
     gene            complement(1078..1977)
                     /locus_tag="VHE33_13595"
     CDS             complement(1078..1977)
                     /locus_tag="VHE33_13595"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVT98532.1"
                     /translation="MREALEYGFTWTLLRLMRALPRGLARGTGAALGAVAWGLLPKLR
                     RTGLRNLELAYPQMPREERVRLLRGLYRYLGWQLGEFCQMPRYTRENTASLIRYEGLE
                     HYLAASARGKGVLIVTGHLGAWELSSFWHSLMGHPMTMVIRRLDNPRVDKMVNDIRCL
                     HGNRVVHKDDFARGLLQAMRRGDTVGILMDTNMTPPQGVFVPFFGVPACTASGLARVA
                     LRTGAAVLPGFLVWEKAEQKYVLHFGPEIPLIATGDDEHDMVANTAQFTAVIEGFVRR
                     YPDQWLWVHRRWKTRPEGEPAIY"
     gene            complement(2092..2766)
                     /locus_tag="VHE33_13600"
     CDS             complement(2092..2766)
                     /locus_tag="VHE33_13600"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HVT98533.1"
                     /translation="MKRFVVILCALAMAVPAFAQKTKLESRLDAARDVLNEVMASPDK
                     QIPDSILKDATCVGVVPGMIKGAFIFGAEYGQGVVTCHTAHGWSAPVFIRMAGGSWGF
                     QIGGQGTDLVLVAVNEKGFQDLLHSQFKIGAGASASAGPVGRNAGASTNWKMQSELLT
                     WSRSKGLFAGVDLSGVAVNQNSSDTDTFYGAPHSFESILRGEVPVPEDARPFVRTVAK
                     YFHEAQ"
BASE COUNT          652 a          996 c          947 g          579 t
ORIGIN      
        1 tacctgatgc tcgcgacagg cgcgacccac tcctatttcg gtaacgatgc atgggagccg
       61 ctggcgcccg gtctgaaaac catcgaagat gccattgaga ttcggcgcag gattctgctc
      121 gccttcgagc tggctgagcg cgagatgctg gaaacgggtt cccatcatgc gctgaatttc
      181 gtcattatcg gcggcggccc taccggcgtg gagctcgccg gcgcaatctg cgacatcacc
      241 cgccgctata tgcagcggga cttccggcac atcgatcccg ccaaagcgcg ggtcctgctg
      301 attgaaggtg aaaagcacgt cctggcgaac taccccgagg acctttcgat caaggcagag
      361 gagcagctga aggcgctggg cgttgagata cacggcggca tgcgcgtcac cgatgtccag
      421 cccggctacg tgatcgccgg agggcagcgc atcgaatctg ccgtgaccct gtgggccgca
      481 ggcgtgcagg cgtcaccgct gggcaaaatg ctgggcatgc ccacggacaa acgcggctgc
      541 gtgctcgtta attccacact gaacccggaa ggccaccccg agatcttcgt ctgcggcgat
      601 ctcgcccatg tcgaacagga tgggcgtcag gttcccggag tcgcgcagcc cgccatgcag
      661 atgggcgatc acattgcgaa aatgatcgca gccgatctca atcatcagcc gcgtccgcct
      721 tttcactatt tcgacaaggg cgatctggcc accattggcc gcctcgctgc tgtgggcaat
      781 gtgaagtggc ccttccgggg gcacctgagc ggatttccag cgtggatcac ctggctggtc
      841 gtccacatct acttcctcat cggtttccgc aatcgcatag ctgtcctgtc gcaatggatc
      901 tgggcctatt tcaccttcac gtatggcgtg cgcctgatct acggcagcca ggctctgccc
      961 gggtggaccg atgaaaacgg ggtcgacaag agcccgggag cacacccact cgatcacacg
     1021 acgccgggtt cgcataccga ggttccgtca aaaagcgccg cggcggacta gcggcggtca
     1081 gtagatcgcc ggctcgccct cgggccgcgt cttccagcgg cgatggaccc agagccactg
     1141 gtccggatag cgccggacga agccttcaat gacggccgtg aactgtgccg tgttcgcaac
     1201 catgtcgtgc tcgtcgtcac cggtcgcgat cagcggaatc tccggaccga aatgcagcac
     1261 atatttctgt tcggccttct cccagacgag aaaacccggc aggactgcgg cgccggtgcg
     1321 cagcgccaca cgcgccagtc ctgaagcggt gcatgcgggc acgccgaaga aaggcacgaa
     1381 aactccctgc ggcggagtca tgttggtgtc catcaggatg ccgacggtat cgccgcgccg
     1441 catcgcctgc agaagcccgc gcgcgaagtc atccttgtgc acgacgcgat tgccgtggag
     1501 gcagcggatg tcgttgacca tcttgtcgac gcgagggtta tcgagacggc ggatcaccat
     1561 cgtcatcggg tggcccatca gcgaatgcca gaagctggag agctcccatg cgccgaggtg
     1621 gccggtgaca atcagaaccc cttttccgcg cgcgcttgct gcaagatagt gctccagtcc
     1681 ctcgtagcgg atgagactgg ccgtgttctc gcgcgtgtat cgcggcatct ggcagaactc
     1741 gcccaactgc cagccgagat accggtagag cccccgcaac aggcgcacgc gctcttcacg
     1801 gggcatttgc gggtaggcca gttccaggtt gcgcagtccg gtgcggcgca gcttcgggag
     1861 caaaccccat gccacagctc cgagagcagc gccggtgccc ctcgccagac cgcggggcaa
     1921 ggcgcgcatc agccgcagca gggtccaggt gaatccatat tccagagctt cccgcatcga
     1981 tggcgaagag ccagtctaca tgggtgcccg cgacgtacag aagcaactgc ggtcaaacgc
     2041 acaaacgcgg aggcgtggag cctccgcgtc tggtttttgc gtgaatcgcg tttactgcgc
     2101 ttcgtggaaa tacttggcca cggtgcgaac gaacggccgt gcatcctccg gcaccggcac
     2161 ctctccgcgg agaatgctct caaacgaatg aggcgcaccg taaaaagtgt ccgtgtcgga
     2221 gctgttctgg tttaccgcga cgccgctcag atccacacct gcaaacaggc ctttgctgcg
     2281 cgaccaggtg agcaactcag actgcatctt ccagttggtc gaagcgccgg cgttgcggcc
     2341 aaccggtccc gcagaagccg aggcgcctgc gccgattttg aactggctgt gcagcagatc
     2401 ctgaaagccc ttctcattca ccgcaaccag aaccagatcc gtgccctgcc caccgatctg
     2461 gaagccccac gatccacccg ccatgcggat gaaaaccgga gcgctccagc cgtgcgccgt
     2521 atggcaggtc acgacgccct gcccgtactc agcgccaaag atgaacgcgc ctttgatcat
     2581 tcccggaacc accccgacgc aggtcgcgtc tttgagaatg gaatcgggga tctgcttgtc
     2641 gggactcgcc atcacctcgt tcaaaacatc tcgagccgca tcgagccggg attcaagctt
     2701 ggtcttctgg gcaaaagccg gcacggccat cgccaacgcg caaagaatga caacaaatct
     2761 tttcatttca gtaatttctc caatcagaaa cgtgatctga ggaagccctc gttatggtac
     2821 cgcaaacaat agaacaccgc gcagaacgaa ccggttgcgc gacctgcggc ggtcggtgct
     2881 ggaaacagtt tgttttgttc gtggttcgca accgggctcc cggacaggcc gcggtcccca
     2941 gcatagagcg caacgaccgg cgtcgctgct tgaatacgat gcgcgctgag ggatttaggt
     3001 tgaaaaggag acaaggcgaa acttgctgaa aacaaacaaa aaatgggggc tgcggatgca
     3061 gtcgcctgcc agcctcagcc ccgttcccca gatctgcgtt gtaaagtgtc tcagttgtag
     3121 cgagatgccc cgtaacgtcg caagataacg gaggtagagt cgactttcgt aacc
//
LOCUS       DASZYN010000531         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_402879, whole genome shotgun sequence.
ACCESSION   DASZYN010000531 DASZYN010000000
VERSION     DASZYN010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402879"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VHE33_13605"
     rRNA            <1..383
                     /locus_tag="VHE33_13605"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            542..656
                     /gene="rrf"
                     /locus_tag="VHE33_13610"
     rRNA            542..656
                     /gene="rrf"
                     /locus_tag="VHE33_13610"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(778..1344)
                     /locus_tag="VHE33_13615"
     CDS             complement(778..1344)
                     /locus_tag="VHE33_13615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT98534.1"
                     /translation="MAASATLALKAGVWFRRRRLLIVSPDSLGTACPLSGRNSTYRPV
                     QISAAGSMIRRGWGRIVFISTSLDIMMAPAFIAYGMTKACDEAYAAALAASLDGTGVT
                     ANVVLPGGPVATRMADPAGIAKGLLPPDIMAAPITWLASDASDHVTGRRFIAARWNSA
                     LTDAQAAQAAGAPAAWGGHRDSSIRPPE"
     gene            1280..>1520
                     /locus_tag="VHE33_13620"
     CDS             1280..>1520
                     /locus_tag="VHE33_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVT98535.1"
                     /translation="MSRRRRRNHTPAFKARVALAAIKGDRTLAQLAEQFDVHPNQITS
                     WKAQLEEGAADVFGPGGGSGTAQPAVDVKSLHAKIG"
BASE COUNT          300 a          432 c          492 g          296 t
ORIGIN      
        1 gtcggctcat cacatcctgg ggctggagca ggtcccaagg gttcggctgt tcgccgatta
       61 aagtggtacg tgagctgggt tcagaacgtc gtgagacagt tcggtcccta tctgccgtgg
      121 gtgtaggaga attgagagga tctgtcccta gtacgagagg accgggatgg acgtacctct
      181 ggtggagcag ttgtggcgcc agctgcagtg ctgcgtagct atgtacggac gggataaccg
      241 ctgaaggcat ctaagcggga aacccacctc gaaacgagtt ctcccttgag agccgtggta
      301 gaccaccacg ttgataggcc gggtgtgtaa gcgcggtgac gcgttcagct taccggtact
      361 aatcgctcga tcggcttgat cgtctctcat tagtcaatgc tcgtcggcgc gtgtgcgcct
      421 atggcgagaa agaccaggcg caaatagcga aatggcgagt agcgaatagt ggttttccct
      481 attcgccatt cgctactcac tattcgcatg tttgtttgct tcacgttccg ttcttcgccg
      541 acctggtggt catagcgagg agcctatacc cgatcccatc ccgaactcgg ccgtcaaagt
      601 cctctgcgcc gatggtacta agtcttaagg cttggaagag taggtcgccg ccaggtctgc
      661 caagaacgga acgcaaatcc tcttcacgta tttcaaacaa aagccctgcg ggaaaccgcg
      721 gggctttttt gtcatttcga gtgaagcaat cgagcctgaa ggatgcggcc catccagtca
      781 ttctggcggc cggatgctgc tgtcgcgatg gccgccccat gcggcgggcg ctccggcggc
      841 ctgagcggcc tgcgcgtcgg tcagcgcgga attccagcgt gcggcgatga agcggcggcc
      901 ggtgacgtgg tcggaggcat ccgacgccag ccatgtgatg ggcgccgcca tgatatcggg
      961 tggaaggagc cccttcgcta tgccggccgg gtcggccatg cgggtcgcga ccggtccgcc
     1021 gggcagcacc acgtttgccg tcacgccggt gccgtcgagc gacgccgcca gggccgcggc
     1081 atacgcctca tcgcaagcct tggtcatgcc gtaggcgatg aaggccggcg ccatcatgat
     1141 gtcgaggctg gtgctgatga aaacgatcct gccccagccg cgcctgatca tagagccggc
     1201 tgcggaaatc tgcacaggac gataagtgga gtttctgcct gacagcgggc acgctgtgcc
     1261 cagcgaatca ggagagacga tgagcagacg acgacgtcgg aaccacacac cggccttcaa
     1321 agcgagggtg gcgcttgctg ccatcaaggg cgatcgaaca ctggcccagc ttgctgagca
     1381 gttcgacgtt caccccaatc agattacatc atggaaggca cagcttgagg aaggggcagc
     1441 cgacgtgttt ggtcccggtg ggggcagcgg aaccgcgcag ccggccgtcg acgtgaagtc
     1501 gctccatgcc aagatcggag
//
LOCUS       DASZYN010000532         4601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_344809, whole genome shotgun sequence.
ACCESSION   DASZYN010000532 DASZYN010000000
VERSION     DASZYN010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344809"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="VHE33_13625"
     CDS             complement(<1..1311)
                     /locus_tag="VHE33_13625"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT98536.1"
                     /translation="MIEDLRFALRQLHRSPGFAAAVVITLALAIGVNTAVFSLLDGFL
                     LRRLPYPQPDRVAALITHRESKNNPANFNDDDSADLFTWKAIQEGVSSVVPAAGGQPF
                     GGTEGVNLEAGKAQGGAVRYVHNATVSAHYFEVLGIQPVLGRGFTEDEDRPGTGKAAV
                     LSYNLWRTTFREDPSILGKAILLRGAPYTVVGVLPQGAQMPNPADVWTPLMPSDRNGV
                     CAGNNCLILMRLKSGATWDEVRGELAHLPPPDNTNLAKQRVWFYPSPLHFYAGSGMRQ
                     QTQALMAAVGFILLIACANLAGLMLARIHHRSPEIATRLALGASRARILRQLWIENLV
                     LALAGGAAGLGLALALFPAMKHLLPDPMIPIGGFTLDGRVLAFTMVATFLTSVLFGAL
                     PALETRRVDLRSSIASGTRSVAGGSSLVRKVLIGAEVALTVVLLA"
     gene            complement(1351..3825)
                     /locus_tag="VHE33_13630"
     CDS             complement(1351..3825)
                     /locus_tag="VHE33_13630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT98537.1"
                     /translation="MLSDLKYAWRQLRKSPGFALTAVVTLALGIGANTAIFSVMHAVL
                     LRMLPVHDPQQLFYLTHETAPDVVSNTGDYRYCFGINVYNRMRADRSVFADLVAYVPL
                     SFSKVGARYGDTPEEIAADEVSGNFFSALGVPMAAGRAFASVDEDQHSQAAVIGYGFW
                     TRRFQRDPSVIGKAIYVNGVPMTVIGVAGPHFYGVESGGNATDLWIPLQSRTELNAWG
                     VPSTGGVARLYTNPNWWNLMFIARLRPGIGQKQALARMEPLFEHAAWETVGKEVKRGN
                     FDMGLGMVPARGLGTATKDYVEPVRVLMGMVALVLVIACVNIAMLLAARNAAREREFS
                     MRLALGAGRWPLFRQLLGESMLLVGAGAGLGWLFAIQATRLLAAWSGIEVSLAPDSPV
                     LFFTLAITVFAALIFGLAPLRAASNAPVSIVLRSTSGQATESRGRVLSGKVLIAGQMA
                     LCVALLFGAGLLIRTLRNYQTVDLGMQADRVLAFGAHPVGAPDTAQKLTFYRQLIQRV
                     GLLPGVGSVTVAELRPGTGWSDSHDLTIDGHSYPWDDGKNMLQTNTVGPKFFATLGIP
                     MLAGRDFDAGDHQGTMGVAIVNETLVRRYLKGASPIGHTLGQGKGQVTIVGLVRDHKY
                     STADENPTPMAWYCYQQDPNLDNLDVMVRAAGDPMTLLPQIRRIVRELDPNAPVQKPA
                     VLSAQFEGTYLMPTLFARLGAFFGGLAALLVAVGLYGTLAYRVSRRTVEIGVRMALGA
                     PRQQVVWMILRDSLVLIAAGLVVGLPLAWFGSKLMASMLYKLNAHDPISFVVAGVGVL
                     TVSLAAALLPARRAASVEPMQALRNE"
     gene            complement(3863..>4601)
                     /locus_tag="VHE33_13635"
     CDS             complement(3863..>4601)
                     /locus_tag="VHE33_13635"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVT98538.1"
                     /translation="PKVAIVNQSFAKKFYGSPSNALGRLIGTEDKPDMTIVGVVGDIR
                     HQSLRKEIGPGAYLPYGQMDHPVGLAVYVRTVGAPELVEPAIRRAIHDQDPTLVVDEM
                     ETMTTVVDMSASSERSLAILAAAFAVLAAILAAVGLYGVLAYSTQSRTREIGVRLALG
                     APRRTLVTLVVREMAWIAVVAALAGIPATVALARLFTSQLYGVRTWDPFALLAALLLT
                     AIMVVVASALPAQRATAVDPMEALRTE"
BASE COUNT          880 a         1543 c         1435 g          743 t
ORIGIN      
        1 ggccagcagc acgacggtca gcgcgacttc ggcgccgatg agaactttgc gaaccaggct
       61 cgagccgccg gcaacggacc gcgttcccga cgcgatggag ctgcgcagat cgacccggcg
      121 cgtctccagc gccggcagcg ctccgaagag cacgctggtg agaaaagttg ccaccatcgt
      181 gaaagcaagc acgcgtccgt ccagcgtgaa gccgccaatg ggaatcatcg gatccggcag
      241 cagatgcttc atggctggaa acagtgccag ggccaggccg agtcctgcgg cgcctccggc
      301 gagggcaagc accagatttt cgatccatag ctggcgcagg atgcgcgcgc gcgatgctcc
      361 cagcgcgagc cgcgtcgcta tttccggaga gcgatgatgg atccgtgcca gcatcaggcc
      421 ggcgagattg gcgcaggcga tcagcaggat gaagccgacc gcggccatga gcgcctgagt
      481 ctgctggcgc atcccgctgc ccgcataaaa gtgcaacgga ctcgggtaaa accacacgcg
      541 ctgcttcgcc agattcgtgt tatcgggcgg gggcagatgc gccagttccc cgcgcacttc
      601 gtcccaggtt gcgccggact tcagtcgcat caggatcagg cagttgttgc ccgcgcatac
      661 gccattgcgg tccgaaggca tcagcggcgt ccatacatcc gcgggattgg gcatctgcgc
      721 gccctggggc agcacgccca ccacggtgta cggagcgccg cgcagcagga tcgctttccc
      781 caggatcgag ggatcttcgc gaaatgtcgt tcgccacagg ttgtagctca acaccgcggc
      841 tttgccggtt cccgggcggt cctcgtcttc agtaaaaccg cgacccagca ccggctggat
      901 ccccagaacc tcaaagtagt gcgccgagac cgtcgcgttg tgcacgtacc gaacagcgcc
      961 gccctgggcc ttgccggcct caaggttcac gccctccgtg ccgccgaaag gctggccacc
     1021 ggcggcggga acgacggagg aaacgccttc ctggatggcc ttccacgtga acaggtccgc
     1081 cgaatcgtcg tcgttgaagt ttgcgggatt gttcttcgac tcgcggtgcg tgatcagcgc
     1141 ggccacgcga tccggttgcg gatacggcag ccggcgcagc aggaacccgt caagcaggct
     1201 gaaaacggct gtgttgactc caatggccag cgccagcgtg ataactaccg ccgccgcaaa
     1261 gccgggactc cgatgcaatt gccgcaaggc aaagcgaagg tcctcgatca tgcaacctcc
     1321 tacgaagcgt ctgccgtccc ggttccctgc ttattcgttc cgcaacgcct gcatcggctc
     1381 cacgctcgcc gcacggcgcg ccggtagcag agcggcggcc agcgagacgg tcagcacgcc
     1441 cacaccagca acaacgaagc tgatcggatc gtgcgcgttg agtttgtaga gcatggaggc
     1501 catcagcttc gacccgaacc atgccagggg cagaccgacc accagtcccg cggcgatcag
     1561 caccaggctg tcgcgcagga tcatccacac tacctgctgc cgaggcgcgc ccagagccat
     1621 gcgcacgccg atctccacgg tccgccggct tacgcggtac gccagggtgc cgtacaggcc
     1681 aacagcaaca agcaaggctg caagcccgcc gaaaaatgcg ccgagacggg caaagagcgt
     1741 gggcatcaga tatgttccct cgaactgcgc cgagagtacc gccggcttct gcaccggggc
     1801 gttgggatcc agttcccgca cgatgcgccg aatctgggga agcagggtca tgggatcacc
     1861 cgcggcgcgc accataacgt cgagattgtc gaggttggga tcctgctggt agcagtacca
     1921 cgccatgggc gtcggattct cgtctgcggt gctgtacttg tggtcgcgca ccagcccgac
     1981 gatggtgacc tgccccttgc cctgcccgag agtatgccct atcggcgaag cacccttcag
     2041 atagcggcgc accagcgtct cattgacaat cgccacgccc atcgtgccct gatggtcgcc
     2101 ggcgtcaaag tcccgcccgg ccagcatggg aatgccgagt gtggcaaaga acttcggccc
     2161 caccgtgttg gtctggagca tgttcttgcc gtcatcccag ggataggagt ggccgtcgat
     2221 ggtgaggtcg tggctgtcgg accagcccgt gccggggcgc aactctgcca cggttaccga
     2281 tccaacgccc ggcagcagac ctacccgctg gatcaactgc cggtagaagg tcagcttttg
     2341 tgccgtgtcc ggtgcgccca ccggatgcgc accgaaggcc agcacgcgat cggcctgcat
     2401 gcccagatcg acggtctggt aattgcgcag cgtgcggatg agcaggccgg cgccgaagag
     2461 cagcgccacg cacagggcca tttgtcccgc gatgagcacc ttgccgctca gcacgcggcc
     2521 gcggctttcg gtggcctggc cgctggtgga acgaagcacg attgacaccg gtgcgttgct
     2581 ggcggcgcgc agtggggcca gaccaaagat gagcgccgca aaaaccgtga tcgcgagggt
     2641 aaagaacaga accggcgaat ccggcgcgag gctgacctca attcccgacc acgcggcgag
     2701 cagccgggtc gcctggatgg cgaagagcca accgagcccg gcaccggcgc cgaccagcag
     2761 catgctctcg ccaagcaatt gccggaagag cggccaccgt ccggcgccca gcgccaggcg
     2821 catggaaaac tcgcgctccc gggcggcatt gcgcgcggca agcagcatgg caatgttcac
     2881 gcaggcgatc accagcacca gcgcgaccat tcccatcagc acgcgcaccg gttcaacgta
     2941 atcctttgtt gctgtgccca gcccgcgcgc cgggaccata cccagcccca tgtcgaaatt
     3001 gccgcgcttt acttctttgc cgacggtttc ccaggccgca tgctcaaaga gaggctccat
     3061 tcgagccaga gcctgtttct ggccgatgcc cggccgcagc cgggcgatga acatcaggtt
     3121 ccaccagttg ggattggtgt acagccgggc gacaccgccc gtcgacggca ctccccaggc
     3181 attcagttcg gtccggcttt gcagcggaat ccacaaatcc gtcgcattgc cgccggattc
     3241 gacaccgtag aagtgtggac ccgcgacacc gatgacggtc atgggcacgc cattgacgta
     3301 aatcgccttg ccgatcactg aggggtcgcg ctgaaaccgc cgcgtccaga atccgtagcc
     3361 gatcacggcc gcctgggagt gctggtcttc gtccaccgaa gcaaaggccc gaccggccgc
     3421 catcggcacg ccgagagcgg agaagaaatt cccgctgacc tcgtccgccg cgatctcctc
     3481 cggcgtgtcg ccgtagcgcg cgcccacctt gctgaacgaa aggggcacgt acgccaccag
     3541 gtcggcaaaa accgaccggt ccgcgcgcat gcggttgtag acattgatcc cgaagcagta
     3601 ccgatagtcg ccggtgttgc tgaccacatc gggcgcggtc tcgtgggtga gatagaagag
     3661 ctgttgcgga tcgtggaccg ggagcatgcg cagcaggacc gcgtgcatca cgctgaagat
     3721 ggcggtattg gcgccgatgc ccagggcgag cgtcaccacc gctgtgagtg cgaagcccgg
     3781 cgactttctg agttgccgcc atgcgtattt cagatcgctc agcatctctc ctccttgctt
     3841 tgggagcttg ctgttctttg aactactccg tccggagggc ctccatggga tccaccgccg
     3901 ttgcccgttg cgccggcagc gccgatgcca ccaccaccat gatggcggtc agcaataacg
     3961 cggccagcag agcgaacgga tcccacgtcc tcacgccgta caactggctg gtaaacagtc
     4021 gtgccagagc gacggtagcc ggaattcctg ccagagcggc gacgacggct atccacgcca
     4081 tctcgcgcac gaccagagtg accagggtcc gccgcggggc gcccagcgcg aggcgaacac
     4141 cgatctcgcg cgtccggctc tgcgtggagt aggccagtac gccatacaag cccactgccg
     4201 ccaagattgc cgccagcacc gcgaacgccg ccgccagaat cgccagggag cgctcgctcg
     4261 atgcgctcat gtcgaccacc gtcgtcatcg tttccatttc atcaacaacc agcgtcggat
     4321 cctggtcgtg gattgcgcga cggatggccg gctccaccag ctccggcgcg ccaacggtcc
     4381 gcacatagac agcgaggccc accggatgat ccatctggcc gtagggcaga taggctcccg
     4441 gcccaatctc cttgcgcaga ctctggtgcc ggatgtcgcc gaccacgccc acaatcgtca
     4501 tgtccggctt gtcttcggtg ccgatgaggc gccccagcgc gttggaaggc gaaccataga
     4561 acttcttcgc gaacgactga ttgacgatcg ccaccttcgg c
//
LOCUS       DASZYN010000533         1868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_219067, whole genome shotgun sequence.
ACCESSION   DASZYN010000533 DASZYN010000000
VERSION     DASZYN010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219067"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VHE33_13640"
     CDS             <1..300
                     /locus_tag="VHE33_13640"
                     /inference="COORDINATES: protein motif:HMM:NF025668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HVT98539.1"
                     /translation="QVQVDVTNTGKRAGAEVIQLYIRDLVSSVTRPIKELKGFRKIYL
                     KPGETATVTLDITPDALAFFDVNMKHVVEPGDFSIMIGTSSRDEDLHKLTLTVTK"
     gene            340..1707
                     /locus_tag="VHE33_13645"
     CDS             340..1707
                     /locus_tag="VHE33_13645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT98540.1"
                     /translation="MIERLSFAEKFGYGLGDMAANFIFQAMLALQLSYYTDTFGLTAA
                     QAGTLFLVVGLIAAAFNPIMGVIADRTNTRWGKFRPWLLWTAIPFGIIGILTFTTPSL
                     SMSGKVIYAWTTYLLLRLIYAVNNVPYASLTGVLTGDPDERTSIASYRQIFANSAGFI
                     VQSLAIPMVVFFGRGNDARGYQITMGLLSILSVIFFIVAFFATKERIQPNPQQETSLT
                     RDLKDLFSNRPWIILFLVTTFYFAAISMRGSVMLPFFKYIAGNEKLFSWFNGFGLASL
                     IAGVAVSTALTKRIGKRPLFILSMTLSGLFNLAVLVLPPHATVAIIVSEVLRQFSYGT
                     SGPLIWAMMGDVADYGEWKTGRRATGTVTAAVVFALWVGLAIGGAVAGWLFSYYGYVS
                     NAVQTARALDGIRLTAGLWSALFFFATAAFLFLYPISRKLNKNISDELAGRRLNFGAP
                     AASQK"
BASE COUNT          339 a          648 c          485 g          396 t
ORIGIN      
        1 caggttcagg tggacgtcac caacacgggc aaacgcgcgg gcgccgaggt gattcagctc
       61 tacatccgag accttgtcag ttcggtcacg cgcccgatca aagagctcaa gggcttcagg
      121 aagatctatc tcaagcccgg cgaaaccgcg accgtcacgc tcgacatcac gcccgacgcc
      181 ctggccttct tcgacgtgaa catgaagcat gtcgtcgagc ccggcgattt ctccatcatg
      241 atcggtacct cctcacgcga tgaggacctc cacaagctca cgctcaccgt caccaaataa
      301 cgggatgaag gcagaactgc cagaaagaac ggcgcggcta tgatcgaaag actttccttt
      361 gcggagaagt ttggctacgg cctgggcgac atggccgcca acttcatctt ccaggcgatg
      421 ctcgccctgc agctgagcta ttacaccgac accttcgggc tcaccgccgc tcaggccggg
      481 accctcttcc tcgttgtcgg tctcatcgcg gcggctttca acccgatcat gggcgtgatc
      541 gccgaccgca ccaacacgcg ctggggaaaa tttcgcccgt ggctgctgtg gaccgcgatt
      601 cccttcggca tcatcggaat tctcactttc accacgccca gcctcagcat gtcgggcaaa
      661 gtcatctacg cgtggacgac ctatctgctg ctgcgcctca tctacgccgt caacaacgtg
      721 ccctacgctt cgctcaccgg tgtgctgacc ggcgatcccg acgaacgcac cagcatcgcc
      781 tcttaccgtc agatcttcgc gaactctgca ggcttcatcg tgcagagcct cgccatccct
      841 atggtcgtct tcttcggccg cggcaatgac gcgcgcggct accagatcac catgggcctg
      901 ctctcgatcc tcagcgtgat cttctttatc gtcgcttttt tcgcgaccaa agaacgtatt
      961 cagcccaacc cgcagcagga gacctcgctc acccgggacc tgaaagacct cttcagcaat
     1021 cgcccctgga tcatcctgtt cctggtcaca acgttttact ttgccgccat ctccatgcgc
     1081 ggcagtgtca tgctgccctt cttcaaatac atcgccggca acgagaagct cttcagctgg
     1141 ttcaacggat tcgggctcgc ctccctcatt gcgggtgtcg ccgtctctac agcgctgacg
     1201 aaacggatcg gcaaacgacc gctcttcatc ctgagcatga ctctttccgg gcttttcaac
     1261 ctggccgtgc ttgttctgcc cccccatgcc accgtcgcca tcatcgtttc cgaagtcctg
     1321 cgccagttct cgtatggaac ctccggcccg ctgatctggg ccatgatggg cgatgtcgcc
     1381 gactacggtg aatggaagac tggtcggcgc gccaccggaa ctgtgacagc ggcggtggtc
     1441 tttgcactgt gggtcggcct cgccatcggt ggcgccgtcg ccggctggct cttctcgtat
     1501 tacggctacg tctccaatgc cgtgcagacc gcgcgcgctc tcgacggcat tcgcctgaca
     1561 gcgggcctct ggtcggcgct gttctttttc gccacggcgg cgtttctctt cctctacccg
     1621 atctcgcgaa agctcaacaa gaacatctcc gacgaactcg ccggcaggcg cctgaatttc
     1681 ggcgcgcccg ccgcatcgca gaaatagatc gcctcgaagg gagcgtcctc gtgtccgtac
     1741 gcatcgcagc actcgccaca cttctcctcg gcttcgcggc tgccgctcag gcgcccacct
     1801 cgctaaagca ggcttatcat ggcgattttt acgtcggcgc cgccatcaac accgcgcaga
     1861 tcaccggg
//
LOCUS       DASZYN010000534         1888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_219122, whole genome shotgun sequence.
ACCESSION   DASZYN010000534 DASZYN010000000
VERSION     DASZYN010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219122"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VHE33_13650"
     CDS             <1..346
                     /locus_tag="VHE33_13650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HVT98541.1"
                     /translation="SVSISGDDLRQILTNIVSNACDALPESGGMIRIDAACADGKATI
                     TVADNGHGISPENLQRIFDPFFTTKADVGTGIGLWVSKDLVEKSGGHVTVETSNLPPG
                     FRTLFRIELPCA"
     gene            complement(365..1045)
                     /locus_tag="VHE33_13655"
     CDS             complement(365..1045)
                     /locus_tag="VHE33_13655"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HVT98542.1"
                     /translation="MPGLLLKAALLTAIWLAWWWAIAARLSSAVNLALIAGGALVVYP
                     VAWVGRKILDRHTTPGAAEWTTTLVQCGLGLGLTISLIRALLTQREWGGWVLPIPKTM
                     GVVLMWITGAACAATVLNLALKGLGAPFFIVLSRRVATDWLYARTRNPMALAVVAFLL
                     SLGIWLQSALFVLWAVVLFAPALLFFIRVFEERELEIRLGAGYLEYKARTPMLIPAVH
                     RTQRSVTR"
     gene            1140..>1888
                     /locus_tag="VHE33_13660"
     CDS             1140..>1888
                     /locus_tag="VHE33_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98543.1"
                     /translation="MSHRKRRHLHGRPLPASFSNPCDLRAGGAPLSTVSVPVQTEAAS
                     VDPWSFATRIAFRFCFVYFGLYCLSTQILTSLLANPKYDFPDPSSIPPWKQIIAFTAT
                     RIFRHKAALVFTGSGSGDKTVDFVLTFCILIVSLLATLIWSLLDRRRTRYPALHRWFL
                     LLIRFSLASQMLIYGFDKAFPLQMPFPGLFTLIEPFGNMSPMGVLWSSVGASQPYEIA
                     AGCFEILGGLLLFFPRTVTLGALVSLVDMSYV"
BASE COUNT          357 a          722 c          440 g          369 t
ORIGIN      
        1 cagtgtttcc atttccggcg acgacctccg ccagattctc accaacatcg tctccaacgc
       61 ctgcgacgct ctgcccgaat ccggcggcat gatccgcatc gacgcggcct gtgccgacgg
      121 caaagccacc atcaccgtcg ccgacaacgg ccatgggatc tcgccggaaa acctgcagcg
      181 catcttcgat cccttcttta ccaccaaggc cgacgtcggc accggcatcg gcctttgggt
      241 ctccaaagac ctggtggaga aaagcggagg ccacgtgaca gtcgaaacca gcaacctccc
      301 gcctggcttt cggaccctct tccgcatcga gctgccctgc gcctgagtgc tgccgtcgga
      361 gctctcaccg agtaacggat cgctgggtcc tgtgaactgc gggaatcaac atgggcgtcc
      421 tcgccttgta ttccagatag cccgcgccga ggcgaatctc cagctcccgc tcctcaaaca
      481 ccctgatgaa gaaaagcaat gccggcgcaa acagcaccac cgcccacagc acaaacaacg
      541 ccgactgaag ccagatcccc agcgagagca gaaacgccac caccgccagc gccatcggat
      601 tgcgcgtgcg tgcgtagagc caatctgtcg ccaccctccg gctcagcacg atgaagaacg
      661 gcgcgcccag ccctttgagc gccagattca aaacggtcgc cgcgcaggcc gcccctgtga
      721 tccacatcag gaccacgccc atagtctttg gaatcggtaa gacccatccg ccccactccc
      781 gctgggtaag aagcgctcga atcagagaaa tcgtcagccc caatcccaaa ccgcactgca
      841 ccaacgtcgt cgtccactcc gcggcgcccg gggttgtgtg ccggtcgagg atcttccgtc
      901 ccacccacgc caccggatat accaccagcg ccccacctgc gatcaacgcc agattcaccg
      961 cgctcgatag tcgcgccgcg attgcccacc accaggccag ccagatcgcc gtcagcaacg
     1021 cagccttaag caataacccc ggcatccgtc gcacagcatc gccctccttc gctccgccaa
     1081 gccatcgagg ctaagttaat cccgcatcgc ccctccgctc gtcgcgaatt tcgcaatgtt
     1141 tgtcccatcg caaacgacgc catttacatg ggcggcccct tccggcatca ttctcgaatc
     1201 cctgcgatct ccgcgccgga ggtgcgcctc tgtccaccgt ttccgttcca gtccaaaccg
     1261 aagccgcttc cgttgatccg tggagcttcg ccacccgcat cgccttccgt ttctgcttcg
     1321 tttacttcgg cctctattgc ctctccacgc agatcctcac cagcctgctt gccaatccca
     1381 aatacgactt tcccgacccc tcctcgattc cgccctggaa acagatcatc gcgttcacag
     1441 caacacgcat cttccgccac aaggcggccc tcgtcttcac cggcagcggc agcggcgaca
     1501 agaccgtcga cttcgtgctg accttctgca tcctcatcgt ctccctcctt gccacgctca
     1561 tctggtcgct cctcgatcgc cggcggaccc gctaccctgc ccttcatcgc tggtttctgc
     1621 tgttgatacg cttttctctc gccagccaga tgctgatcta tggcttcgac aaagccttcc
     1681 ccctgcagat gcccttccca ggcctcttca ccctgattga gcccttcggc aacatgtctc
     1741 ccatgggcgt cctctggtct tcggtcggag cgtcccagcc ctacgaaatc gccgccggct
     1801 gcttcgaaat cctcggcgga ctgctcctct tctttccacg taccgtcacc ctcggcgcac
     1861 ttgtttcgct cgtagatatg agctacgt
//
LOCUS       DASZYN010000535         8619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1245757, whole genome shotgun sequence.
ACCESSION   DASZYN010000535 DASZYN010000000
VERSION     DASZYN010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8619
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245757"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="VHE33_13665"
     CDS             <1..1572
                     /locus_tag="VHE33_13665"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVT98544.1"
                     /translation="ERAKSRLAELQSAIPGSGRVRYDQLTEAQRALHHAAFVTNAASP
                     DFHELEPLPFTDNGKTETMVVPKGDIFSQLRKDVNEGKLPTISWLTAPEKFSDHPTSP
                     WYGAWYVSEVMDILTRNPEVWKKTIFILTYDENDGYFDHAPSFVAADPKRPQTGGASP
                     GIDMGLEYTYVEDELAQGVAQQYARSGPIGMGFRVPMIIASPWTRGGWVNSQLFDHTS
                     TLMFLENFIGKKYGKEVKEENISSWRRAVAGDLTSVFRRYNDKEAQLDFLDRDKFVVG
                     IEQARYKEIPSNYRNLSTNEIADINRNSQHSRFMSHQESGTRPACALPYELYAEGGLT
                     SDGTEFELRLTAGNHVHGNSAAGAPFNVYLHGLKDASSGGFQAATFAVKAGGSLQPRF
                     PLSLFGDKGYWIEVHAPNGFYRSFTATPGSPPIEIRAEYERSGTSLSRNLLVHVHNSS
                     SNPMTATIVESSYRTSTHRKTIRPNEIWSRRLHLKDSHGWYDFTVKAGGSEARFAGRV
                     ETGRPSISDPLMAMV"
     gene            complement(1544..2944)
                     /locus_tag="VHE33_13670"
     CDS             complement(1544..2944)
                     /locus_tag="VHE33_13670"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HVT98545.1"
                     /translation="MERISNGGKRFLTIAVIAVLSWGFLYAQSSADVTVLKGLAPVTA
                     LMNTSAGKAALGANFSVTGGIQTGSIRQPTLFPFAEQQQQALRDAFITDRNLAELADG
                     LGTTLGSAYVARFHSIDEKQTSRLPESLGDLIRYATAVSGAHSNEGKYLFANGTTDGK
                     TPVPSAEADIVSAAGGIEDVFGHAYHLQAGSPGADPYGNSRPYQTEPNVTSFAGPDYL
                     DHPADNFVYNHGPVMDLTNSPSYPSGHTTYGYTGAVLLAVLVPERYAQMIARGAEYGN
                     DRVIMGSHYVMDVLGGRTLALYDMAHLLANDPAYMGLSVRGAAPIADFQAVVKKARAE
                     LDMILKAGCGNTMDACAKEDTGRFSNAEADEAFYDVTQTYNLPAVYPNNAGAEDVSKL
                     APEAGYLLTSAFPSLTLEQADRILTETEGPGGGFLDDGSSFGVYSRLNLYEAGLRAER
                     AAASHEGLRPSPSTGR"
     gene            complement(3100..4314)
                     /locus_tag="VHE33_13675"
     CDS             complement(3100..4314)
                     /locus_tag="VHE33_13675"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HVT98546.1"
                     /translation="MKNRAVLISGASVAGPALAFWLHRYGFQPTIVERAPAVRPGGYA
                     VDFRGASMRVLERMGLLEAIREKQTRIGTITIVDRNNRKVASMPDGFTSGELEILRGD
                     LAALFHQATRDTTEYLFNDSIAAIEEDSDGVEVRFERGGRRRFDLVVGADGLHSRVRS
                     LAFGDEANFVRYLGYYVSIFTIPNYLHLDHAGLYYGALGKKVGIFSGGDPGEAKASFF
                     FASEALDDSRRNTAEQKRILRSQFEREGWELPRLLELMETAPDFYFDSVSQVRMDRWS
                     KRRIVLLGDASWCASPLSGMGTGMAVVGAYVLAGELAEAEGDYRIAFQQYESRMREFV
                     RKCQGIAEGGTDWFVPRTRFRLWLSNQMWKILPYTPWKNMMIEVPLKIGNSIELKDYQ
                     QFESVDESLSPV"
     gene            4396..5052
                     /locus_tag="VHE33_13680"
     CDS             4396..5052
                     /locus_tag="VHE33_13680"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT98547.1"
                     /translation="MRIAKSSGNRDALLLAAKQCLLEKGYNRTTARDIAALAGVSLAA
                     IGYHFSSKEALLTEALLLAFGEWDQDLQQTLRTAVPTDTDPAKRFEVIWETIMATFET
                     HRALWVANFELFAQIVAQPAAREVIVGKLHVARAGLAAMFLNQDESTIDDRTVRTVGT
                     FLHVLLSGVVLQWLMDPSSALSARNLTVALRDIAQELGKHQKKSSARSKSRTGKRPRK
                     "
     gene            complement(6033..6413)
                     /locus_tag="VHE33_13685"
     CDS             complement(6033..6413)
                     /locus_tag="VHE33_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98548.1"
                     /translation="MMRIQESKRHRERFRNQSGGGSAPHFFLTSGSGHFCRQIPQSAA
                     VAIVENTLGCFQCRNQHTAYAPIVCGRWTVGKGEIALFQIVVAVQNEQLIGNGEILAA
                     GHHIVEVRPYEVHCFGEDLADGTA"
     gene            6843..>8619
                     /locus_tag="VHE33_13690"
     CDS             6843..>8619
                     /locus_tag="VHE33_13690"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT98549.1"
                     /translation="MSHEFRTPLTLMLGSLEELSARGTSRDPEERQQLDVARRNALRL
                     LKLVNTLLDFSRIEAGRVQAVYEPTDLRTLTAEIASSFRSAMEKAGLRFLVNCEPIEE
                     PIYVDRDMWEKIVLNLLSNAFKFTFAGEVHVSLRPVNHSVELSVRDTGVGIEKQEQPR
                     IFERFHRVESAHARTYEGTGIGLALVQELVKLHGGEVHVESELGRGSTFTVIIPRGAS
                     HLPAKQIGAGRTLASTALSPDAYRHEAERWLPEGAVATDAEDTVPLPSSAVTPTLTAP
                     EREHIVVADDNADMRDYLAHLLSKHYTVHAVADGSQALEAVRQLNPILVVADVMMPML
                     DGFGLVHAIRSDPSLCSTPVILLSARAGEESRVEGLQGGADDYLVKPFTARELIARVT
                     THVKMARLRRDAAQRESRLRAEDERERRRLEELLAQAPAAIALLTGSDHRWIYVNENY
                     IRLTGRSSAADFLGKTLVESLPELETQVFVGLLNEVYRTGKPYFGREMMAILNRSASG
                     LPDESYWDFVYQPVRDVDGIVEGILVHAVEVTDKVAARNAVKENAERLLLAQTAAQIG
                     TWEWDPERDSPHLSSELHHLFGTAAE"
BASE COUNT         1835 a         2503 c         2499 g         1782 t
ORIGIN      
        1 gaaagagcca aaagccgcct ggcggagctt cagtcggcaa ttccaggcag tggccgcgtc
       61 cgctatgacc agctcaccga ggcacaacgc gcgctgcacc acgcagcatt tgtaaccaat
      121 gccgcgagtc ccgattttca cgaactggag ccgcttccgt tcaccgataa cggcaagacg
      181 gaaacgatgg tcgtcccgaa gggagatatc ttcagccagc tccgcaaaga tgtaaacgag
      241 ggcaagcttc caaccatttc ctggctcacg gctcctgaga agttctccga tcaccccacg
      301 tcaccctggt atggtgcatg gtacgtgtcc gaggtgatgg atattctcac caggaatccc
      361 gaggtttgga agaagacgat tttcatcctg acttatgacg agaacgacgg ctacttcgat
      421 cacgcgccgt ctttcgtggc ggccgatccg aaacgaccgc agacgggcgg tgcatcccca
      481 ggaatcgaca tgggtctcga gtacacctat gtcgaggacg agctggctca gggagtagct
      541 cagcaatatg cccggtccgg gccgatcggc atgggcttcc gcgtaccgat gattattgcc
      601 tctccgtgga cgcgcggggg atgggtgaat tcccagcttt tcgatcacac atccacgctg
      661 atgtttctcg agaacttcat cgggaagaag tacggcaagg aggtgaaaga ggaaaacatc
      721 agttcctggc gccgcgcggt ggcgggagat ctgacctcgg tgtttcggcg ctacaacgac
      781 aaagaggctc agctcgattt tctcgatcgc gacaaattcg tggttggcat cgagcaggcg
      841 cggtacaaag aaatcccctc caactacaga aacctctcca cgaacgaaat tgcggatatc
      901 aatcgcaact cgcaacactc gcggttcatg tcgcatcagg agtcaggaac gaggccggcc
      961 tgtgcgctgc cctatgagct ctatgcggag ggcggactga cttcggatgg aactgagttc
     1021 gaactgcgcc tgacggcagg aaaccacgta cacggcaact cagccgccgg agcgcccttt
     1081 aatgtgtacc tgcacggtct gaaggatgct tccagcggcg gatttcaggc tgcgaccttt
     1141 gcggtcaagg ctggcggttc acttcagccg cgctttccct tgtctctttt cggtgacaaa
     1201 ggctattgga tcgaggtcca tgccccgaac ggcttctatc gctcgttcac agcaaccccc
     1261 ggttccccgc ccatcgaaat ccgtgcggaa tacgagagga gtggaaccag cttgagcagg
     1321 aatcttttag ttcacgtgca taactcaagt tcaaacccga tgaccgcgac catcgtggag
     1381 tcctcgtaca gaacgagcac acaccgcaag acgattcgcc cgaatgagat atggtcgcgg
     1441 cgccttcacc tgaaagacag ccatggctgg tatgacttca cggtgaaagc aggcggatcg
     1501 gaggcgcgat ttgccggccg cgtcgaaaca ggacgaccga gcatcagcga cccgttgatg
     1561 gcgatggtct gagcccttcg tgggaggcag ctgccctctc agcccgcagc cccgcttcgt
     1621 aaaggttcag ccgcgaatac acgccgaaag aggatccatc gtcgaggaac cctccgcccg
     1681 gcccttctgt ttctgtgagg atccgatccg cctgctccag ggtgagcgat ggaaaggcgg
     1741 aagtcagcag gtacccggcc tcaggcgcca gcttgcttac atcttcggct ccagcattgt
     1801 ttggatagac cgcaggcaaa ttgtaggtct gcgtcacgtc gtaaaacgct tcgtccgcct
     1861 ctgcattgct gaaccggccc gtatcttctt tggcgcaggc gtccatggtg tttccgcatc
     1921 cggccttcag aatcatgtcc agttccgcgc gcgctttctt caccacggcc tgaaagtcgg
     1981 caatcggcgc cgcccccctg accgacaagc ccatatacgc cggatcattg gccagcaggt
     2041 gcgccatgtc gtacagagcc agcgtgcggc cgcccaacac atccatcaca taatgcgacc
     2101 ccatgatcac gcggtcgttt ccatattccg ctccgcgcgc aatcatctgg gcatatcgtt
     2161 cgggaaccag cacggccagc aggactgcgc ctgtatagcc ataggtggtg tggccgctgg
     2221 ggtaggaagg actgttcgtc aggtccatga ccggaccatg gttatagaca aagttgtccg
     2281 ctggatgatc gaggtaatcc ggtcctgcaa acgatgtgac gttgggttcg gtctgataag
     2341 gacgcgaatt cccgtacgga tcggccccgg gcgatccagc ctgcaagtgg taggcgtggc
     2401 cgaacacgtc ctcgatgccg ccggccgcgc tgacaatgtc tgcctcggcc gatggcaccg
     2461 gagtttttcc atccgtggtc ccgttcgcga acagatattt cccctcgttc gaatgcgcgc
     2521 cggagaccgc tgtcgcatat cggatcagat cgcccaggga ttccgggagc ctggaggttt
     2581 gcttctcatc gatagagtga aagcgtgcaa catacgcaga gcccagcgtg gtcccaagcc
     2641 cgtcggcgag ttcagcaaga ttgcggtcag taatgaaagc atcccggagc gcctgctgtt
     2701 gctgttccgc gaaaggaaac agcgttggtt ggcgaattga gcccgtctga attccgcccg
     2761 tgacggaaaa attggctccg agtgctgcct tgccggcgct cgtattcatc agcgccgtta
     2821 caggagcaag acccttcaga acggtgacgt ccgcagacga ttgagcgtaa agaaagcccc
     2881 acgaaaggac cgcgataact gcgatggtga gaaatctttt gccgccgttc gagattcgct
     2941 ccattcgcat attgtctacc aaaagccgca gcaaaggcgg agccggacag tatcaggagt
     3001 ttcgatcgtg cgatcgatct cctgacaggc ctggcgaaca tgcagcgatc ggcgacgcgt
     3061 ccccttccgg aaagggcagc gtgcccactc ctgtcgccgt cagaccgggc tcaatgactc
     3121 gtctacggat tcgaattgct gataatcctt taattcgatc gaattcccga ttttcagagg
     3181 aacttcaatc atcatgttct tccagggcgt atagggcagg attttccaca tttgattgct
     3241 caaccacagc cggaaacgcg ttcgcggcac aaaccagtcg gtaccgccct ccgcaattcc
     3301 ctggcatttg cgcacaaact ctcgcattcg gctctcatat tgctgaaagg cgatccgata
     3361 gtccccttcg gcttccgcga gttcgcccgc cagaacgtag gctccaacaa cggccatgcc
     3421 tgttcccatc cccgagagag gcgaggcaca ccagcttgca tcccccagca acacgatgcg
     3481 gcgcttcgac catcggtcca tcctgacctg gctgacggaa tcgaagtaga aatccggagc
     3541 cgtctccatt agttccagca atctgggcag ctcccaccct tcacgctcga attgcgaacg
     3601 caggatcctt ttctgttccg cggtgtttcg gcgtgaatcg tcgagggctt cgctggcgaa
     3661 gaagaacgat gcctttgcct ccccgggatc gccgccgctg aaaatgccca ccttcttgcc
     3721 cagcgctccg tagtaaaggc cggcgtggtc aagatgcaga tagttcggaa tcgtaaagat
     3781 tgacacgtag tacccgagat accgaacgaa gttggcctca tctccaaatg cgagggagcg
     3841 cacccgggag tgcaacccgt cggcgcccac caccagatcg aatctgcgcc ggccgccgcg
     3901 ctcaaaccgg acctccacac catcgctgtc ttcctcgatt gcggcgatgc tgtcgttgaa
     3961 aaggtactcg gtggtgtccc gtgtggcttg gtggaagagg gcggccaggt cgccgcgcag
     4021 aatctcgagt tctccgcttg taaagccgtc gggcatgctg gcaactttcc tgttgttgcg
     4081 atcgacgatc gtaatggtcc cgatgcgggt ctgtttttcg cgaatcgcct ccagcagtcc
     4141 catacgttcc agcacgcgca tcgaggcgcc gcgaaaatca accgcatagc cgcccggtcg
     4201 caccgcaggc gcgcgttcga caattgttgg ctggaatccg tagcgatgca gccagaaggc
     4261 cagggctgga cccgccacgc tggcgccaga aatcagaacg gcacggtttt tcatgatgac
     4321 tactccggaa cgaatgggtg ttgtacatct gtcttataca tttgtaccac acgtatgtct
     4381 ggtacaaatg ttttcatgcg aattgcaaaa tcgtcaggca atcgcgacgc tctgctgctg
     4441 gccgcgaaac aatgcctgct ggaaaagggc tacaaccgca ccaccgctcg agatatcgcg
     4501 gccctggccg gggtcagcct cgccgctatc ggctaccact tttcctcaaa ggaagctctg
     4561 ttgactgaag ccctgcttct ggcgtttggc gaatgggatc aggacctgca acaaacgctg
     4621 cgcacggccg ttccaacgga tacagacccg gcgaagagat tcgaggtcat ttgggaaacc
     4681 atcatggcca ccttcgaaac ccatcgtgcg ctgtgggttg cgaatttcga acttttcgct
     4741 caaattgtgg cgcagccggc cgcgcgagag gtaattgttg gcaagctaca tgtggctcgt
     4801 gcgggattgg ccgcgatgtt tctcaatcag gatgaaagca caatcgacga tcgcaccgtg
     4861 cgaaccgtgg gaacgtttct ccacgttttg ctttccggag tggtcctcca gtggctcatg
     4921 gatccgtcca gtgcgctgag cgcgaggaat ctaacggttg cgcttcgcga catcgcacag
     4981 gaacttggga aacaccaaaa gaaatccagc gcgcgttcga agagccgcac aggaaagcgc
     5041 cctcggaaat gagacagacg acgaggcaga gatcgcatcc tgccggttcc ggggcgccgt
     5101 cgcaaacgtg tcatgtgtgg tgattcgaag gcgctcgtac ccgaattcct gatcggccga
     5161 ggctgcgacc aactggtcaa cgaggcgatc agcaggttcg ccgacaagcg gaaactcgtg
     5221 aggacaacgg ctaagttgtg gttttgattg gctgcccccc agggattcga accccgatat
     5281 gctgatccag agtcgggaaa ggggtgctga ttctacgcag gatcaacagg ttagcccggc
     5341 aaaatccggc aatttacggc gaatcccgca acccaggcgc aacccttaac ggccccggct
     5401 tcctggacga aacccacttt cgcattgcgt acagtcgtgt ccggatgaaa cgcaccgaat
     5461 cgaagcggcg tctgcgatgt acccgccgtt cctaaaccgg cgaaggtgaa ttcccacata
     5521 ttctgtcgca gccgagggct gatttcctgc cggcggctga aacgagtgag gcaactgcca
     5581 agacataatg tgtcatcaaa atgtgcgctt cgtcctatcg ggagttgcct gctccaggcc
     5641 ggtggattgg actctccctc ccatccgtga tctgagatgc aggtgactac ggttgtcggg
     5701 agactactcc attgagttcg tgggacatag ggcaagaatc cattccgacg gctgagcagc
     5761 catcgacagt gaacctcgtt gagcccgacg aatcggttct ggcccaatcc gtcctctccg
     5821 gcggtggcca aatgggcgcc ctaatgcgcg ccttcgattg ggcgcagact ccgctcggcc
     5881 cggtgtcact gtggccacaa tcacttcgga ttgcggtcag aattctgctg ggcacgggct
     5941 accccatgct gatctgctgg ggcagccagt acaccatgat ctataacgac ggctaccgcc
     6001 cgatcctggg aaaatccaaa cacccggccg cgttaggcag tcccatcagc caggtcttct
     6061 ccgaagcaat ggacttcata gggccgcact tcaacaatgt gatgacccgc ggcgaggatt
     6121 tcaccgttac cgatcagctg ttcattttgg accgcaacga ctatctggaa gagtgctatt
     6181 tcaccttttc ctacagtcca gcgcccgcag acgatgggag cgtaggcggt gtgctggtta
     6241 cggcactgga aacaaccgag cgtattctcg acgatcgcca ccgccgcact ttgcgggatc
     6301 tggcgacaga aatggcccga gcccgaagtg aggaagaagt gtggcgcgct gccgccgcca
     6361 ctctggtttc tgaaccgctc gcgatgcctt ttgctctctt gtattctcat catccgaccg
     6421 agcgccaggc gcggctggtt ggaagcgctg gcggaacgag tgcctcaatg aggcccccgg
     6481 tcatcgatct tgaaaaggga ggcgtttggc cgtttgagcg cgctctggca aattcggccg
     6541 agttgatgct ggatgatctg gattcgcgct ttgctgatgt gccgctgacc tcctggggtg
     6601 ttcctccgaa aaaggccttc attgtgccga tacttttgag agaacatcgt gagcccgctg
     6661 gattcttagt cggaggcatt tcaccacggc gggagttcga tgcgacatat cgcgaatttg
     6721 tggagaggag cgcggagcag atcgcgaccg ggctaaccag cgcccgaacg tatgaagagg
     6781 aacgtcgccg ggccgaggca ctggcggaac tcgatcgtgc aaagactctg ttcttcagca
     6841 acgtgagcca cgagttcagg acacccctaa cattgatgct ggggtcactt gaggaactat
     6901 ctgcgagggg aacttctcgt gaccctgaag aacggcaaca gcttgacgtt gcccggcgaa
     6961 acgctcttcg gctgctcaag ctggtcaaca cgctgctcga tttttcacgt atagaggcag
     7021 gcagagtgca ggcagtctat gaacccactg acctcaggac attgacagcc gaaattgcca
     7081 gctcattccg ttccgcgatg gaaaaggctg gtctccgctt cctggtgaac tgcgagccga
     7141 tcgaggagcc gatctatgtg gatcgggaca tgtgggagaa gatcgttctc aatcttctct
     7201 ccaatgcttt caagttcacc tttgcgggag aggtgcatgt ctctctgagg ccggtgaacc
     7261 attcagtcga gctttccgtt cgagacacgg gcgttgggat tgagaagcaa gaacaacccc
     7321 gaatctttga gcgattccat cgcgtcgaga gcgctcacgc caggacatat gagggcaccg
     7381 gcatcgggct cgctttagtg caggaactgg tgaagctgca tggcggcgag gttcacgtcg
     7441 agagtgaatt gggccgcggc agcaccttca cggtaatcat cccccgcggt gcatctcacc
     7501 tgcccgcgaa gcagatcgga gccgggcgga cgctggcttc gacagcgctt tcaccggatg
     7561 cgtaccggca cgaagctgaa cgctggttgc cggaaggagc tgtcgcgaca gatgcagagg
     7621 acaccgtgcc tctcccgtca tctgcggtca cgcctactct gacagccccc gaaagggagc
     7681 acatcgttgt agccgatgac aacgcagaca tgcgcgacta tttggcgcac ttgctgagca
     7741 agcattacac agtgcatgca gtcgcggacg gatctcaggc tctggaggcc gtccggcaac
     7801 taaatccgat actcgtcgtg gcggatgtca tgatgcctat gctggacggg tttggtctgg
     7861 ttcatgcgat tcggtctgac ccgtccttat gcagcactcc cgtcatcctg ctttcggcgc
     7921 gcgcgggaga ggaatcgcgc gtcgaaggtc tgcaaggggg agcggatgat tacctcgtaa
     7981 agccttttac tgcgcgcgag ctgattgcgc gggtgacgac tcacgttaag atggccaggt
     8041 tgcgtcggga tgccgcgcaa cgagaatcgc ggctccgcgc ggaagatgag cgtgaacgtc
     8101 gccggctgga agaactgttg gcacaggcgc cggcagccat cgctctgctg actggatctg
     8161 atcatcgctg gatctatgtc aacgagaact acatcaggtt gacgggaaga tcgagcgcgg
     8221 cagatttcct tggcaaaacg cttgtggaaa gcctgccgga actggaaacg caggtcttcg
     8281 tcggccttct caatgaggta tatcgcaccg gcaagcccta ttttggccgc gaaatgatgg
     8341 cgattctgaa ccgttcggcg agcggactgc cagacgagtc gtattgggat tttgtatatc
     8401 agcctgtccg cgatgtggat ggcattgtcg agggcattct ggtccatgcc gtcgaagtta
     8461 ctgacaaagt cgccgctcgc aacgccgtca aggaaaatgc ggagcgattg ttgctggcgc
     8521 agactgccgc ccagatcggt acatgggaat gggatcccga gcgggactca ccccatcttt
     8581 cgtctgagct gcaccatctt ttcggcaccg cagcggagg
//
LOCUS       DASZYN010000536         2743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_541189, whole genome shotgun sequence.
ACCESSION   DASZYN010000536 DASZYN010000000
VERSION     DASZYN010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541189"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            345..1547
                     /locus_tag="VHE33_13695"
     CDS             345..1547
                     /locus_tag="VHE33_13695"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVT98550.1"
                     /translation="MYMPLLMYGYAVALVLMLLGCRLAMRTVPGLAGLPSLSFAIALG
                     LVSVIGFSVRPWAPAWITIMIANLALFAAMLLLYCAAADTVAARRKFLPSGIALWLAS
                     GAGFAWFTWIHPALTPRILMSSSLCAVYAGAAAWVLYRHRGVAGQGTSATMLRSLISS
                     MVWLESAVVALHTVRCVLTVCFPPSSFIHLDSIQAGFSYLNLVLVLGNICGLIWLSLC
                     RHRAELQVLAQTDGLTGLLNRRAFEQILARELARAQRGGDSLAVLLVDIDRFKEVNDT
                     LGHLAGDEVIRRVSAVLRGGLRPADALGRYGGEEFVCMLRESSPDQAEEVAERLRVEI
                     AGLSAMPGGLRITASIGVAASQPTEDIEQLLRRCDDALYGSKRRGRNLVTIHRAFPAS
                     SDVSIQPA"
     gene            1566..>2743
                     /gene="miaB"
                     /locus_tag="VHE33_13700"
     CDS             1566..>2743
                     /gene="miaB"
                     /locus_tag="VHE33_13700"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HVT98551.1"
                     /translation="MPGLVGYTRGMAGQKTFYLETFGCQMNVHDSEKVIGTLEQQGYA
                     RVETEEAADLILYNTCSIRDKAEQKVFNRLNDYKALYKKGKRFGVLGCVAQQEGERIF
                     ERAPYVSLVSGSASYRRLPEMLVQIEQGAQRVTGLDDRQTEETFETEFTARSNPHRGY
                     ITIIEGCDKFCAYCVVPYTRGKERSRKSDSVLVEARRMADAGFTEIQLLGQNVNSYRD
                     PEGKRSFAELLAAVGQVAGIRRVRFTTSHPRDFTKDIVEAIDAVPSLCDHVHLPVQSG
                     SSKVLRLMAREYSREQYLERIAWIQGAKRTISMTTDIIAGFPGETVEDFEETLSLLSE
                     VQYDGVFAFKYSPRPNTPALAMADSIPEEEKSKRLQVLLDRQREIQRTNYERHVGEIL
                     "
BASE COUNT          482 a          801 c          900 g          560 t
ORIGIN      
        1 ccataatgca tggcggccgg cgcctcctgc caggtcgaga gcgcggcctg gagccaaatc
       61 cgatccaacc tccactacga acgggtccgg ttccaccagc tgcgcctctc cagcgaggaa
      121 tttcgaattc caccgttcgc ggtctgtcgt tttgccggtt ctgtctgtca tcgccttcgc
      181 gttgccaccg cttgtattct ccccttttgg gggatttgtt gaagttgctg ataacgatag
      241 agtaacgtca gcgttcggtg ccacggttcg cgaatgctgc acgccggccc ctgcgcagtc
      301 ccgcaagatc aagtccgtcc gacacaagga gttcgttcgc gcgcatgtac atgccgcttc
      361 tgatgtacgg gtatgcggtc gccctggtgc tgatgctgct gggctgccgg ctcgcgatgc
      421 gcacggtgcc cggcctcgcc ggcctgccct ctctttcctt cgcaatcgcg ctcggcctgg
      481 tgtccgtcat tggcttctca gtgcggccgt gggcgccggc ctggatcacg atcatgatcg
      541 cgaacctcgc tctttttgct gccatgctgc tgctctattg tgcggcggcg gatacagttg
      601 cggcgcgcag gaaattcctg ccctcgggga tcgcactctg gctggcgagt ggcgcaggtt
      661 ttgcgtggtt cacctggatc catccggcgc tgacgccacg gattctgatg agcagcagtc
      721 tgtgcgctgt gtacgccggc gcagcggcct gggtgcttta tcggcaccga ggtgtggccg
      781 gtcagggaac ctctgcgacg atgctgcggt ccctcatttc ctccatggtt tggctggagt
      841 cagcggtggt ggcgctgcac acggttcgct gcgtgctgac tgtctgcttt ccgcccagca
      901 gtttcattca cctcgactcc attcaggcgg gattcagcta cctgaatctg gtgctggttc
      961 tgggcaacat ttgcggcctc atctggctgt cgctgtgcag gcaccgggcc gaactgcagg
     1021 tgctggcgca aaccgacggc ctgacgggtc tgctgaaccg cagagcgttc gagcagattc
     1081 tcgcgcgcga gctggcacgg gcgcagcgcg ggggagactc gctggccgtg cttctggtcg
     1141 atatcgaccg cttcaaggaa gtgaacgaca ccctgggcca cctggccggc gacgaagtga
     1201 tccgccgggt gagcgcggtg ctccgcggag gccttcgtcc cgccgatgcc ctcggccgtt
     1261 atggcggcga ggagttcgtt tgcatgctgc gggaaagcag ccccgaccag gcagaggaag
     1321 tggccgagcg gctgcgggtt gagattgccg gactttccgc gatgcctggc gggctccgaa
     1381 tcaccgccag cattggggtc gcggccagcc agcccacgga agacatcgag cagttgctcc
     1441 gccggtgcga tgacgccttg tacggatcca agcgccgggg aagaaatctg gtgacgatcc
     1501 atcgcgcttt ccccgcatcg tccgatgtct cgattcaacc cgcctgagcc tccggggggc
     1561 agaaaatgcc cggattggtc ggctatactc ggggaatggc ggggcagaaa accttctatc
     1621 tggagacctt tggctgccag atgaacgtgc atgactcgga gaaagtcatt ggcacgctgg
     1681 agcagcaggg ctacgcccgg gtagagaccg aggaagccgc cgacctgatc ctctataaca
     1741 cctgctcgat ccgcgacaag gcggagcaga aggtcttcaa ccgcctcaac gattacaagg
     1801 cgctttacaa aaagggcaag cggtttggcg tgctgggatg tgtggcgcag caggaaggcg
     1861 agcgcatctt cgagcgggcc ccgtatgtct cgctggtatc gggatcggct tcgtatcgac
     1921 gattgccgga gatgctggtg cagatcgagc agggcgcgca acgcgtgacc gggctcgacg
     1981 accggcagac cgaggagacg ttcgagaccg aattcacggc gcggtcgaat ccacaccgtg
     2041 gctacatcac gatcatcgaa ggctgcgaca agttctgcgc gtactgcgtg gttccttaca
     2101 cgcggggcaa ggaacgcagc cgcaagtccg actcagtcct ggtggaagcg cgccgcatgg
     2161 ccgatgccgg gtttacggag attcaactgc tgggccagaa tgtgaattcg tatcgggacc
     2221 cggaaggaaa gcggtcgttt gcagaattgc tggccgctgt cggtcaggtt gccggtattc
     2281 gccgtgtgcg ttttacgacc tcgcatccca gggactttac gaaggacatc gtcgaggcga
     2341 tcgacgcggt accttccctg tgcgatcacg tgcatctgcc ggtgcagagc ggctcatcga
     2401 aggtattgcg tttgatggcg cgtgaatatt cgcgcgagca gtatctggaa cgcatcgcat
     2461 ggattcaggg cgcgaagcgg acgatcagca tgacgaccga tatcattgcg ggattccccg
     2521 gggaaacggt ggaggacttc gaggagacgc tgtcgctgct gagcgaggtt cagtacgacg
     2581 gcgtgttcgc cttcaagtat tcaccgcgtc cgaatacgcc ggcgctggct atggcggatt
     2641 cgattccgga ggaggaaaag tcgaagcggc tgcaggtgct gctcgatcgg caacgcgaga
     2701 tccagcgaac gaactacgag aggcatgtcg gggaaattct gga
//
LOCUS       DASZYN010000537        12080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_219208, whole genome shotgun sequence.
ACCESSION   DASZYN010000537 DASZYN010000000
VERSION     DASZYN010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 12080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12080
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219208"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VHE33_13705"
     CDS             complement(<1..1073)
                     /locus_tag="VHE33_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98552.1"
                     /translation="MTVSKAATMAALAMALMALPVHAQSFGAAKTKVNLDRKLPALYH
                     LPGKTIRVRVTGKADETDLANDLQTQLETELLKDDPQLQDEENNPDVTINCQITNFDH
                     PAPIFIKQPSVGNQPPSVNQRITGHLGLSFQAKARDGRVLGSDNVQVNYDQQFDSSGN
                     NVSHGIMGSLHTSWHKLKGGKGNDDQEDQAPTPPELRSLLLISAVHRIAEQVVNTNET
                     IEVFLAKNKGPMDERDKAAEAGLWERALETFETAPPLSKRDEDAYRLYDIGVANEALG
                     YAAGDSKSAMKFLQEAAIHYGKAIDAKPSEKYFLGPQKRIETAILHYRKLEQEKNAPP
                     PAVVAAENPPPAPKSAIDPPASNP"
     gene            complement(1541..2014)
                     /locus_tag="VHE33_13710"
     CDS             complement(1541..2014)
                     /locus_tag="VHE33_13710"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT98553.1"
                     /translation="MQAIKPFLWFSDKAEEAMNFYVSVFKNAKAGSVARYPEGAPGKA
                     GSVMTATFTIGDIEFIALNGGPGDPFNNAVSLVVPCDTQAEIDELWAKLTEGGEEIQC
                     GWLKDKFGLRWQVVPSNMGELLQGKDAEGAKSAMQAMMQMKKLNIAALRRAGGLQ"
     gene            complement(2026..2364)
                     /locus_tag="VHE33_13715"
     CDS             complement(2026..2364)
                     /locus_tag="VHE33_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HVT98554.1"
                     /translation="MIRVELPHHLRVLAQIGSEVTLEVADPVTLQRVLDALEERYPVL
                     RGTIRDHDTKKRRPFLRFFACQEDLSHDSPDELLPEAIATGREPLLVIGAIAGGCSPA
                     RQSNATVNFE"
     gene            complement(2361..3539)
                     /locus_tag="VHE33_13720"
     CDS             complement(2361..3539)
                     /locus_tag="VHE33_13720"
                     /inference="COORDINATES: protein motif:HMM:NF014110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98555.1"
                     /translation="MSQVRVLVGTKKGAFILNADGKRENWDVSGPFFAGWEVYHMKGS
                     PVNPNRIYVSQTSSWSGQVIQRSDDGGKTWSAPGGGVQKSPQGWPMGESNRFVYDTSA
                     ETGRPLTTHQFYDGTQHPWEFKRVWHLEPSLTDAEAVYAGVEDAAIFHSKDGAHSWQE
                     MPALRGHGTGPKWQPGAGGMCLHTIVLDPKDPNRIYIAISAAGAFRTDDGGKTWKPIN
                     KGLRSQYIPDQDAEVGHCVHHIAMNPRRPGVLFMQKHWDVMRSDDAGDSWREVSGNLP
                     TDFGFVIDVHAHEPETIYVAPIKSDSEHYPLDGKLQVWRSKTGGNEWEALTNGLPQQN
                     CYVNVLRDAMAVDSLDKCGVYFGTTGGQVYVSPDAGDTWKPIVRDLPAVLSVEVQTLS
                     "
     gene            complement(3666..4052)
                     /locus_tag="VHE33_13725"
     CDS             complement(3666..4052)
                     /locus_tag="VHE33_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98556.1"
                     /translation="MKLQPYLYFKGNCEEAFQLYARVLGGDYQGVFRYGQSPMAGQHG
                     KEWDDKVMHVSMNIGDQTLMGSDAPTSHFRQPQAMPYVCTAPTKRRRSVFTARSPKAA
                     KSICLCRRLSGRSAMPCLRIASARNG"
     gene            complement(4113..4523)
                     /locus_tag="VHE33_13730"
     CDS             complement(4113..4523)
                     /locus_tag="VHE33_13730"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT98557.1"
                     /translation="MKLYTHLNFGGNCREAFEFYEQKVGGKITMMMTQGDMPSAGRAP
                     GTKSSAIAHARMNIGESELIGNDVPAEHFQPMRSAYLYLAVDSAEEAERIYSVLSERG
                     EVFMPLEETFYASRFAMLRDRFGVSWTLICERAR"
     gene            complement(4626..5156)
                     /locus_tag="VHE33_13735"
     CDS             complement(4626..5156)
                     /locus_tag="VHE33_13735"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HVT98558.1"
                     /translation="MSIAQSFLAEFEQQAPVTRRFLERLSDDKLLWQPHEKSMTAGQL
                     ALHLGMVPGAVITLVQNASAPPPDFTVRPQPANSGEVLSALDESIAAVRTLLPKLDDV
                     AMQETFRIVAGDQDLMALPRQAFIRNIMLNHWYQHRGQFSVYLRLLGIAVPASWGPSA
                     DEAPVFSRQPEVVAAS"
     gene            complement(5189..5626)
                     /locus_tag="VHE33_13740"
     CDS             complement(5189..5626)
                     /locus_tag="VHE33_13740"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HVT98559.1"
                     /translation="MESTMEAKVREAEVIPTVSAGRLWTGRVIAGLIAAFMMMDAAIK
                     FIKPPQVAEAFVRTGWPLELSAPLGAILLSSLVLYLIPRTSILGAILLTAYFGGAVAT
                     NLRLENPWLTYTLFPVYFGVLTWLSLWLREPRLHEWIPLRRPR"
     gene            complement(5696..6397)
                     /locus_tag="VHE33_13745"
     CDS             complement(5696..6397)
                     /locus_tag="VHE33_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98560.1"
                     /translation="MTVFRRIALALAIVVAGLASAPAQNTQASYPKMAPLDQYLIADR
                     NAEIALARTAAPRSISDNAEVMVLGKDGYEVAVQGTNHFVCFVERSWDAAIGDPVFWN
                     PKIRGPNCVNAAAARSWLPIALMKTRLAVSGQSQQKIDQAMQAAFRSKKLPELEPGSM
                     CYMMSKQGYLNDAARNWHPHLMFFVPLAMSKTWGANLSGSPVFSGDDVSDHLTIFMIP
                     VAHWSDGTPDTGGTK"
     gene            complement(6429..6935)
                     /locus_tag="VHE33_13750"
     CDS             complement(6429..6935)
                     /locus_tag="VHE33_13750"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVT98561.1"
                     /translation="MAVKRLPTHRSGCPVNISLEVFGDRWSLLIVRDLMVRGFTRFQD
                     FVESGEGIATNILADRLKNLQRCGVVTSEEDQEDGRRVIYRLTEKGIDLAPVMLELLI
                     WGAQHENTGAPPAVIAELAKNRKAVLEETRRRWRERDPAPLLPWLGGPKRTARPRAVL
                     GERQQGRK"
     gene            complement(7078..8721)
                     /locus_tag="VHE33_13755"
     CDS             complement(7078..8721)
                     /locus_tag="VHE33_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HVT98562.1"
                     /translation="MKKVWIFGALLACCGCLCAQEQNPTATEHWSQNGQQPQGQTEAG
                     IPIYRINVVSRDLPAINYFHRNGSTKIGFEGTPLLPMAKGRAEVNSLNGRTEIKAHFE
                     GLGPANGFGIEYLTYVLWAISPEGRPINLGEVLWTHDHADTTVTTNLQSFGLILTAEP
                     YFAVTVPSDLVVMQNYVLKDKTSGVLETVNAHYTLLPRGAYADTAGRHTVLHPITRDE
                     HSPLELYEAINAVQIAQAAGAEQYAPDAMANAKLDLKNAQDMDIHQKEMKQEITYARE
                     AVQTAEDARIITIRKIKANDEAAARAKIEAARHKAEDDAAAAQAQAQQAADEQAAAQA
                     RAEAARAQAEAAREETEKARAQQQAAEAAAAAAARQESEMREKLKQQLNSVLQTQESA
                     RGLIVNMSDVLFAFNKYELKSDAQIKLAKISGILLTYPNLKVQVEGYTDNIGSDEYNL
                     KLSQERATAVQAFLVEQGVQPGNVSAQGYGKADPVADNATSAGRAANRRVSLVVSGAS
                     IGVQEQGPAPGSSMAPPPAGAPPQQPAPATGTSNTEPQD"
     gene            8941..9246
                     /locus_tag="VHE33_13760"
     CDS             8941..9246
                     /locus_tag="VHE33_13760"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HVT98563.1"
                     /translation="MPVAVMVLELRIEHAHSLKDRRQVVRSLKEKLAHGFNVSIAEMD
                     EAVTWQSATIGIAAISASRDYLTGQMQQVENAAVRIAEGLGAQISASWEFLESDPLP"
     gene            9335..9964
                     /locus_tag="VHE33_13765"
     CDS             9335..9964
                     /locus_tag="VHE33_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98564.1"
                     /translation="MTKNISGPWAVLTTLTFSLFTLPALAQQHPEGGHPTQQHPSSGS
                     HGSQGSGSHSNPHVPVRPVGPARPPIGQGHIPSHGPQTHTPLPRNDDHDQHGHSNAPY
                     VDAHTDRWVGHVPDNRKAYHLDHPWEHGRFPGTTGAHQIWRLAGGGPSRFSFGGFFFS
                     VAPADLGYCDGWDWNSDDIVLYPDPDDPGWYLAYNARLGIYVHVMYLGS"
     gene            10127..11635
                     /gene="malQ"
                     /locus_tag="VHE33_13770"
     CDS             10127..11635
                     /gene="malQ"
                     /locus_tag="VHE33_13770"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HVT98565.1"
                     /translation="MPFERSSGLLLHIASLPSYGGIGDLGPAAYAFADFLAASKQRLW
                     QVLPLGPTGYGNSPYAALSAFAGNPLLISLEFLADRGWIGHERIEGLPGRSGPVDHEA
                     VTAKKRPLLEEAARNFLRQHDAQEWSAFEAYCATNATWLNDWALYSVLRRKFDYASWN
                     DWPSEIKHRDAAVLERVRQEHGEELATVQAMQFAFNEQWRALRDYCAARDIRFIGDIA
                     IFVNYDSADVWTHPELFELNPDLSPIRVAGVPPDYFSATGQRWGNPLYKWNVLQQSGF
                     AWWVDRIRRAQFLYDVIRLDHFRGFEAYWAIPAQDETAVNGQWIKAPGAALFQTLRER
                     LGELPFIAEDLGLITKEVDALREQFGLPGMKVLQFGFSDRGAHIHLPHRFVTNSVAYT
                     GTHDNDTTRGWWENGASQTEKAAAGVYLGVKDDDIVWPLIRSTARSVADLALYPVQDI
                     LELSSEARMNVPSRPTGNWSWRCPENALTPELAQKLGAITEVTDRDKHERPS"
     gene            complement(11797..>12080)
                     /locus_tag="VHE33_13775"
     CDS             complement(11797..>12080)
                     /locus_tag="VHE33_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98566.1"
                     /translation="VTATNGMAEHVLGFTRGGRVLAVVPRFAYTLMGGKARLPLNNAW
                     SRGDLLVPEMAKASLENVFTGEIVRVDDDGRLPLKTVFGEFPVFLGVRG"
BASE COUNT         2190 a         3724 c         3588 g         2578 t
ORIGIN      
        1 ggattcgacg ccggcggatc gattgccgat ttcggcgcag ggggcgggtt ttctgctgcc
       61 accacagccg gcggcggagc gttcttctcc tgctccagct tgcgatagtg cagaatcgcg
      121 gtttcgattc gtttttgcgg ccccaggaaa tacttctcgc tcggttttgc gtcgatggct
      181 ttgccgtagt gaatcgcggc ttcctgcagg aatttcatgg ccgacttcga atcgccggcg
      241 gcgtatccca gcgcttcatt cgcgacgccg atgtcgtaga ggcggtaggc gtcctcgtct
      301 cgtttcgaga ggggaggcgc ggtttcaaat gtctcgagcg cccgctccca caggcccgcc
      361 tcggctgcct tgtctcgttc atccatcggc cctttgtttt tcgcgaggaa gacctcgatg
      421 gtttcgttgg tgttcactac ctgctctgcg atgcgatgca cggccgagat cagcagcagg
      481 gaacgcagtt cgggcggggt gggcgcctga tcctcctgat cgtcattgcc cttaccgccc
      541 tttaacttgt gccatgaagt gtgcaggctt cccatgatgc cgtgggagac gttgttgccg
      601 gagctatcga attgctggtc gtagttcacc tgcacgttgt cggagcccag gacacggccg
      661 tcgcgcgcct tcgcctgaaa cgagaggccg aggtgaccgg tgatgcgctg attcacggag
      721 gggggctggt tccctaccga cggctgtttg atgaagatcg gcgcggggtg atcaaaattt
      781 gtgatctggc agttgatcgt cacgtcagga ttgttttctt catcctgcag ctgtggatca
      841 tccttcagca gctcggtttc cagctgcgtc tgcagatcgt tcgcgaggtc tgtttcgtcg
      901 gccttgccgg tcaccctgac ccgaatcgtt tttccgggca ggtgataaag cgcgggcaat
      961 ttccggtcga ggtttacctt cgtttttgcg gcaccaaagc tctgcgcgtg gaccggcagc
     1021 gccatgaggg ccattgccag ggcagccatc gtcgcagctt tacttacagt cattgctcac
     1081 tccctcggtt ctgaggcccc accgttttac cggggatcat ccattttcta acggtgtgtg
     1141 cctggttcca ctggtggctc tccgtcgaat taccgcgcgt cctgcgatcc tgtttctcgc
     1201 tatggttgaa acagcgagtt tcacatcctg gcctgaatcc ccgtccagga gccgttttac
     1261 cgggcactcg ggcgagattc tctcgttttc ctgggaaggg gaacccatca tagcaatttc
     1321 tctgcgcaat ccaatacggt tttctttgcg ctcaaaaatt gtccctgggc gcctgagccc
     1381 ctgacattcg tcggcagtga aaaacaaata aggcgcgatc ggaatcgatc gcgcccaggt
     1441 gacttcggag ttgttgaaag gttttgtgtt agcgaagaaa tacgggattt ctcaggccag
     1501 gtaacaagta acgcacgtaa tcacttgtcc acttgcggag ttactgcagc ccacccgcgc
     1561 ggcgcagggc tgcgatgttc agttttttca tctgcatcat cgcctgcatg gcacttttcg
     1621 cgccttccgc atcctttccc tgcagcagct cgcccatatt cgaaggaacc acctgccaac
     1681 ggagtccgaa tttgtccttc agccatccgc actgaatctc ttccccgccc tcggtcaact
     1741 ttgcccacag ctcatcgatc tcggcctgcg tatcacaggg gacgacgagc gagacggcat
     1801 tgttgaatgg atcgccaggg ccaccgttca gcgcgatgaa ctcgatatcg ccgatggtga
     1861 aggtcgcagt catgaccgat ccggccttcc cgggtgcgcc ctccggatag cgagcgacgc
     1921 ttcctgcttt tgcgttctta aaaacggaga cgtagaagtt catcgcctct tctgctttgt
     1981 cgctgaacca caagaatggc ttgatggcct gcatcggatc tctcctcact caaaattaac
     2041 ggttgcgttc gactgacgtg cgggcgagca cccgccggcg atggctccga tcaccagcag
     2101 gggctctctt cccgttgcta tggcttcagg caggagctca tccggagaat cgtgggacag
     2161 gtcctcctgg caagcgaaga agcgcagaaa gggacggcgc ttcttcgtgt cgtgatcgcg
     2221 tatcgttccg cgcagcaccg gatagcgctc ttcgagcgca tcgagcacgc gctgcaacgt
     2281 caccgggtcc gcgacttcga gcgtgacttc actaccgatc tgcgccagca cgcgcagatg
     2341 atgcggcaac tccacacgga tcatgagagg gtctggacct ccaccgaaag cacggccggc
     2401 agatcgcgca caatcggctt ccaggtgtcg ccggcatccg gggagacgta gacctgtccg
     2461 ccggtggtgc cgaaatagac gccgcatttg tcgagcgaat cgacggccat ggcatcgcgc
     2521 agcacgttga cgtaacagtt ctgctgcgga aggccgttgg tcaacgcctc ccactcattg
     2581 ccgccggttt tgctgcgcca cacctggagt ttgccgtcga ggggatagtg ctcggagtcg
     2641 ctcttgatcg gcgccacgta aatcgtctcc ggttcgtgtg cgtggacgtc gatgacaaat
     2701 ccgaaatcgg tggggagatt tccgctcact tcgcgccacg aatctccggc gtcgtcgctg
     2761 cgcatcacgt cccagtgctt ctgcataaac agcacgccgg gtcgccgcgg gttcatggcg
     2821 atgtggtgga cgcaatggcc cacttcggca tcctgatcgg ggatgtactg ggagcgcagc
     2881 cctttgttga tgggcttcca cgttttgcca ccgtcatcgg tgcggaacgc gccggcggct
     2941 gagatcgcga tgtagatccg gttgggatcc ttcgggtcga gcacgatggt gtgcaggcac
     3001 atgccgcctg cgcccggctg ccacttgggc cccgttccgt ggccgcgcag tgccggcatc
     3061 tcctgccacg aatgcgctcc atccttcgag tggaagattg cggcatcttc cacgccggca
     3121 tagactgcct cggcatcggt gagcgacggc tccagatgcc acacccgttt gaactcccac
     3181 ggatgctggg tgccgtcgta gaactgatgg gtggtgagag gccttcctgt ttcggcggag
     3241 gtgtcgtaga cgaagcggtt ggattcgccc atgggccagc cctggggact cttctgcacg
     3301 ccgccgcccg gagcggacca tgtttttccg ccgtcatcgg aacgctggat cacctgcccg
     3361 gaccagctgc tggtctgcga gacgtagatg cggttgggat tcaccggcga tcccttcatg
     3421 tgatagacct cccaacctgc gaagaaaggt ccactcacgt cccagttctc acgcttgccg
     3481 tctgcgttca gaatgaacgc gccttttttg gttcctacca gtactcgcac ctggctcatg
     3541 caatctcctc cctttcagat cctcgtgggt gcgacgccca agacaactcg cagctatgag
     3601 cgccgcgttc gattccctgt tgctgcctct cacgccgatg cttcacatcg gcttcgagac
     3661 gttgatcatc cattgcgtgc cgaagcgatc cgtaaacatg gcatagcgct tcgcccagaa
     3721 agtctcctgc agaggcatat cgattttgcc gccttcggag agcgggctgt aaatacgctc
     3781 cgcctccgct tcgtcggcgc agtgcagaca tacggcatag cctgcggctg gcggaaatgc
     3841 gaggtcggcg catcgctgcc catcagggtc tgatcgccga tgttcatgga gacgtgcatg
     3901 accttgtcat cccattcttt gccgtgctgg ccagccatgg ggctctgccc gtaacgaaac
     3961 acaccctgat agtcgccgcc cagaaccctt gcatacaact ggaaggcctc ttcgcagttg
     4021 cccttgaaat agagatacgg ttgaagcttc atcctcactc cttatccatg gcgaaatggt
     4081 tgtcctcgat tgcaatcgca ccgatcatga cgtcatcttg cccgttcgca gatcagtgtc
     4141 cacgaaacgc caaagcggtc gcgcaacatg gcaaaccgcg acgcgtagaa ggtctcttcc
     4201 agcggcatga acacctcgcc ccgttcgctc agaacggagt aaatgcgctc ggcttcctcc
     4261 gccgaatcca cggccagata caggtaggcg ctgcgcatcg gctgaaagtg ttcggccggc
     4321 acatcgttcc cgatcagctc cgactctccg atgttcatgc gcgcatgcgc aatggcgctg
     4381 gatttggtgc ccggggcgcg gcccgccgac ggcatgtcgc cctgggtcat catcatggtg
     4441 atttttccgc caaccttctg ctcgtaaaat tcgaaggctt cgcgacagtt cccgccgaaa
     4501 ttcagatggg tataaagttt cattcttctc ctctgggaat caggacctcg atccctgttc
     4561 gttcgtgttc cgcagcgaaa cagcagaacc ggttcactga ccagcggtgc tcgtggcggc
     4621 caccgtcaac tcgccgcgac tacctccggt tgccgggaaa aaacaggtgc ttcgtcggcg
     4681 ctgggtcccc agcttgcggg aaccgcaatt cccagcaggc ggaggtaaac gctgaactga
     4741 cctcgatgct gataccagtg gttcagcata atattccgga tgaatgcctg gcgcggcaac
     4801 gccatcagat cctgatcgcc agcgacgatg cggaaggttt cctgcatcgc cacgtcgtcc
     4861 agtttcggca agagcgttct gacggcagcg atgctttcgt cgagtgcgct caggacctca
     4921 ccactgttgg ccggttgtgg gcggacggtg aagtccggag gcggcgccga cgcgttctgc
     4981 accagcgtga tgacggcccc gggcaccatc ccaaggtgca atgcgagctg cccggcggtc
     5041 atggattttt cgtgcggctg ccagagcagc ttgtcatcgg aaaggcgctc caggaatctg
     5101 cgggtaacgg gggcctgctg ctcgaactct gcgagaaacg actgcgcaat cgacatacac
     5161 caactccttt tttcaacgat ggaatgactt agcggggcct gcgcaacgga atccactcat
     5221 gcagccgcgg ctcacgcagc cagagcgaca gccaggtgag gacgccgaaa tagacaggaa
     5281 acagcgtgta ggtgagccat ggattctcga gccgcaggtt ggtcgccact gcgccgccga
     5341 aatacgcggt gagcaggatt gctccgagga tcgaggtgcg cggtatcaga tagaggacca
     5401 gggaactgag cagaattgcg cccagggggg cggagagctc caggggccag ccagtccgca
     5461 cgaaggcctc tgccacctgg ggcggcttta tgaacttgat cgccgcatcc atcatcatga
     5521 acgcggcgat gaggccggcg atgacgcggc cggtccagag ccggccggca gaaacagtcg
     5581 ggatcacttc cgcttccctc accttcgctt ccatcgtcga ttccatcccc gcttctcctc
     5641 cataaccact taagttgcgc ttctactttt tggccagcca ccgggcgcgg gcgctctact
     5701 tcgttccgcc ggtgtcgggc gtgccatccg accagtgagc gacgggaatc atgaagatcg
     5761 ttaggtgatc agaaacatcg tcaccggaaa agacgggtga accagagaga tttgcgcccc
     5821 acgttttcga catggcgagg ggaacgaaaa acatgaggtg cggatgccag ttccgcgcag
     5881 catcgttcag gtagccctgt ttggacatca tgtagcacat cgaacccggc tccagttcag
     5941 ggagtttctt gctgcggaag gcggcctgca tggcctgatc gatcttctgc tgcgattggc
     6001 cggagacagc gaggcgagtc ttcatcagcg cgatgggcag ccaggatcgg gcagccgctg
     6061 cattgacgca gttagggcca cggattttcg gattccagaa cacaggatca ccgatcgcgg
     6121 catcccagga tcgctcgacg aagcacacaa agtgattggt gccctgaacc gcgacctcgt
     6181 agccgtcttt gccgagaacc atcacttcgg cgttgtccga gatcgacctg ggcgcggccg
     6241 tccgcgcgag agcgatttcg gcgttgcgat ccgcgatcag atactgatcg aggggagcca
     6301 tcttggggta cgacgcctgc gtgttctgag ccggcgccga cgcgaggccg gcaactacga
     6361 tcgccagcgc gagggcgatc ctgcggaaaa cagtcatgtc gcttcccctc cttcggtgac
     6421 cacgttcttc atttccggcc ttgctgccgt tcgccaagga ctgcgcgtgg acgagcggtc
     6481 cttttgggcc cacccagcca gggcagcaag ggcgcaggat cccgctccct ccaccgccgc
     6541 ctcgtttcct ccaggaccgc tttccgattt ttggccagct cagcgatcac agccggcggt
     6601 gcgcccgtat tttcgtgctg tgcaccccag atcagcagtt ccagcatcac cggcgccaga
     6661 tcgatgccct tttccgtcag gcgatagatc acgcgccggc cgtcctcctg gtcttcctcg
     6721 ctggtcacga ccccgcatcg ctgcaggttt ttcagccggt ccgcgaggat gttggttgca
     6781 atgccctcgc ccgactccac aaaatcctga aaccttgtaa aaccgcgcac catgaggtcg
     6841 cgcacgatca gcagagacca gcggtctcca aaaacctcca gcgaaatatt gaccgggcag
     6901 cccgaacgat gcgtcgggag tcgcttgact gccatgcacg cgacagtagc aatgttcact
     6961 tgctgtttgc aagtgatctt ttccacagtg tgacgaatgg gacagcaaga agcaaacaag
     7021 aagggccgct cggggagcgg cccttgttgc gatccgatga aagcgcgttg ctgtgactta
     7081 gtcctgaggc tccgtattgg aggtacctgt agcaggcgca ggctgctgcg gcggcgcccc
     7141 cgcgggagga ggggccatcg agcttcctgg agcggggccc tgttcctgca ctccgatgga
     7201 cgctcccgaa accaccagcg acaccctgcg gttcgcagcg cgtcccgcac tggttgcgtt
     7261 gtcggctacc ggatcggcct tgccgtagcc ctgcgcgctc acgttgcccg gctgaactcc
     7321 ctgctccacc agaaatgcct gcaccgcggt ggcgcgctcc tgggagagct tgaggttgta
     7381 ctcatcggac ccgatgttgt cggtataccc ctccacctgc accttcaggt tgggataagt
     7441 gagcaggatg cccgagatct tggcgagctt gatctgagca tcggacttca gctcgtactt
     7501 gttgaacgca aagagcacgt cggacatatt cacgatcaaa ccgcgcgcgc tttcctgggt
     7561 ttgcagcacg gagttcagct gctgcttgag cttttcacgc atctccgatt cctgccgggc
     7621 agcggccgcc gctgctgctt ctgctgcttg ttgctgtgcc cgagcctttt cggtttcctc
     7681 gcgcgcggct tcagcctggg cacgagccgc ctctgcacgc gcctgggcgg ctgcctgttc
     7741 gtcggcggcc tgttgcgcct gggcctgggc agcggccgca tcgtcttcag ctttatgccg
     7801 cgccgcttcg atcttcgccc gtgccgcggc ttcgtcgtta gccttgattt tgcggatggt
     7861 gatgatgcgg gcgtcttcgg ctgtctggac cgcctcgcgg gcgtaggtaa tttcctgttt
     7921 catctccttc tgatggatat ccatgtcctg cgcgttcttc agatccagtt tcgcgttggc
     7981 catggcgtcc ggagcgtact gttccgcccc ggcggcttgc gcaatctgca ccgcgttgat
     8041 cgcctcgtag agttcgaggg gggagtgctc gtcgcgggtg atcggatgca gcactgtgtg
     8101 acgccccgcg gtatccgcat aagcgccacg cggcagcagc gtgtagtgcg cattgaccgt
     8161 ttccagcaca ccggacgtct tgtctttcag aacatagttt tgcatcacca ccagatcgct
     8221 gggcacggtg acggcgaagt agggctccgc ggtcaggatc aacccaaagg actggaggtt
     8281 cgtcgtcacg gtcgtatccg catgatcatg cgtccagagc acctcgccca gattgatcgg
     8341 acgcccttcg ggggagatcg cccagagaac gtaggtcagg tactcgatgc cgaatccgtt
     8401 ggctggcccg agtccttcaa aatgtgcctt gatctcggtg cgtccattca ggctgttcac
     8461 ctcggccctg cccttggcca tgggcaggag gggcgtgcct tcaaatccaa tcttcgttga
     8521 gccgttgcga tggaagtagt taatcgcggg aagatcgcgg gacaccacat tgattcggta
     8581 gatgggaatt cctgcttcgg tctgcccttg cggctgctgc ccattctgcg accagtgctc
     8641 ggtggcggtg gggttctgct cctgcgcaca caggcatccg caacaggcca gcagtgctcc
     8701 gaaaatccag actttcttca tctcgcgttc tccttgtggg ggtcaatggg gggacgtgtt
     8761 tgcgtcaccg tccctgggat gcctccgtgg gaggcagaag atggccggtg ggcgctaaac
     8821 gggcatcagg gccatataca tcctttcgga atttcaggcg catctcaagg cggcctcgcc
     8881 agcaaacggg agcacgccca tggatcgtca acaggctcgt ggttgctaag atgaagcccg
     8941 atgcccgttg ctgttatggt tctcgaactc cgcattgagc acgcgcactc gctcaaggac
     9001 cggcggcagg tcgtccgcag cctgaaggaa aagcttgctc acggattcaa cgtctccatc
     9061 gccgaaatgg acgaagcggt cacctggcaa tcggcgacga tcgggatcgc ggccatatcc
     9121 gcttcgcgtg actatctcac cggacagatg cagcaggtgg aaaacgccgc cgtgcgcatt
     9181 gcggaaggcc tgggcgcgca gatcagcgcc tcctgggaat tcctggaatc ggatccgctt
     9241 ccgtgaatgc gcaactgttt cctcggctca tccgtctacc cgtgcgcagt tcggtgtatc
     9301 tatttggctg caacctgctt tcccggagga atccatgacg aagaacatca gcggtccctg
     9361 ggcagttctc accactctta ccttttcgct cttcacgctg ccggctctgg cgcagcagca
     9421 tccggagggc ggtcatccca cgcagcaaca tccgagttca ggctcgcacg gcagccaggg
     9481 gtccggctcg cacagcaatc cgcatgtgcc ggtaaggccc gtgggaccgg cgcgtccgcc
     9541 gatcggccag ggacatatcc cctcccacgg cccgcagacc cacacgccgc tgcccaggaa
     9601 cgatgaccac gaccagcatg ggcactcgaa cgcgccctac gttgacgccc ataccgatcg
     9661 ctgggtgggc cacgtccccg acaatcgcaa ggcttatcac ctcgatcacc cgtgggagca
     9721 cggccgcttc cctggcacaa caggagccca tcagatctgg cggctggccg gcggtggtcc
     9781 ttcgcgcttc tcctttggcg gattcttctt ttctgtggcg cccgccgatc ttggatactg
     9841 cgacggctgg gactggaatt cggacgacat cgtgctgtat cccgatcccg acgatcccgg
     9901 ctggtacctc gcctacaatg cacggctcgg catttacgtt catgtgatgt atctgggatc
     9961 gtagacaacc cctgatgcac tcaggcgtcc gaagcaacgg agcccgtttc ctgggaatct
    10021 ctcccagaga acgggctctg gggcggcgcg tgcaatcccc ggcggctgag aacggcccaa
    10081 agccgaagtc tgcgctttgg tgcacactat tgctgaaggg tcttgcatgc cttttgaacg
    10141 ctcgtcgggc ctcctgctcc acattgcatc cctgccatcg tatggcggca tcggcgacct
    10201 tggtcccgct gcgtacgcct tcgcggactt tttagctgct tcgaagcagc ggctgtggca
    10261 ggttctgccg ctgggtccca ccggatacgg caactcgccc tacgcggcgc tctcggcgtt
    10321 tgcgggcaac ccgcttctga tctcgcttga gtttctcgcg gatcgcggct ggatcggtca
    10381 cgaacgcatc gagggactcc cgggacgctc cggacccgtc gaccacgaag ccgtcacagc
    10441 aaagaagcga ccgctgctgg aagaggctgc ccgcaacttc ctccgtcagc acgacgccca
    10501 ggagtggtct gccttcgaag cgtattgcgc gacgaacgcc acgtggctga atgactgggc
    10561 tctgtactcg gtgctgcgcc ggaaattcga ctacgcttcg tggaacgact ggccgagcga
    10621 gatcaagcac cgcgatgcgg ccgtgctgga acgtgtgcgc caggaacatg gcgaggagct
    10681 ggccacggtg caggccatgc agttcgcctt caacgaacaa tggcgtgcgc tgcgcgacta
    10741 ctgcgccgcc cgcgacattc ggtttatcgg ggacatcgcc atcttcgtca actatgactc
    10801 ggcggatgtc tggacccatc ctgaactctt tgagctcaac cctgatctgt ctccgatccg
    10861 ggtcgcgggt gttccgcccg actatttttc tgccaccggg cagcgctggg gcaatcctct
    10921 ctacaagtgg aatgtgctgc agcaatcggg tttcgcgtgg tgggtcgatc ggattcggcg
    10981 cgcgcagttc ctttatgacg tgattcggct cgaccacttc cgtggtttcg aagcgtactg
    11041 ggcgattccg gcgcaggatg agacggcggt taatggccag tggatcaagg ctccgggtgc
    11101 ggcgcttttc cagaccctgc gcgagcggct gggcgaactt cccttcatcg cggaagacct
    11161 tgggctgatc accaaagagg tggatgcttt acgcgaacaa tttggccttc cggggatgaa
    11221 agtgctgcag ttcggcttct ccgaccgggg cgcccacatc cacttgcctc atcgatttgt
    11281 aacgaactcg gtggcttata cgggcaccca cgacaacgac acgacacgcg gctggtggga
    11341 aaacggcgcg agccaaacag agaaggccgc cgccggcgtg tatctgggtg tgaaggacga
    11401 cgatattgtc tggcctctga tccgctccac ggcacgatcg gtggcggatc tcgccctgta
    11461 cccggtccag gacatcctcg agctttcgag cgaagcccgg atgaacgtcc cgtctcgccc
    11521 gacgggcaac tggagctggc gctgtccgga aaacgcgctc acgcctgaac tcgcgcagaa
    11581 gcttggcgcc atcaccgagg ttacggatcg ggacaagcac gaacgtcctt cgtgaactgc
    11641 gggttggcgg aatcggcgga caagatcagc ggtgaacgaa gagaaagcct caggggctga
    11701 agcccacatc atttttgcgc ttttgcgaca cgactgaagt catgccctgt tacaaaagcg
    11761 ccggccaatt cctcaacgga cttgcgggac accacactag cctctgacgc cgagaaagac
    11821 cggaaattcg ccgaagacgg ttttcagcgg cagccggccg tcgtcgtcga cacgaacgat
    11881 ttcgccggtg aagacattct cgagcgaagc tttggccatt tccggaacca gcaaatctcc
    11941 gcgtgaccat gcgttattga gcgggaggcg cgccttgccg cccatcagcg tatatgcgaa
    12001 tcgcggaacc acggccagca cgcgcccacc gcgcgtgaag ccgagaacat gttcggccat
    12061 tccatttgtc gcagtcacgg
//
LOCUS       DASZYN010000538         1997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_219571, whole genome shotgun sequence.
ACCESSION   DASZYN010000538 DASZYN010000000
VERSION     DASZYN010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219571"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..437)
                     /locus_tag="VHE33_13780"
     CDS             complement(33..437)
                     /locus_tag="VHE33_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98567.1"
                     /translation="MCSPRATGSRQASWMTWARWRGGNPLGPPRAVGVGQQSSEAALL
                     VAATAAPDGGAVALPAGGDTVNRFARGDGQDDASALDLEERARGLASDALEAEEVAGG
                     EGKGARFATAHGGSLSIGEVEALLHLQHSGCP"
     gene            418..1215
                     /locus_tag="VHE33_13785"
     CDS             418..1215
                     /locus_tag="VHE33_13785"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVT98568.1"
                     /translation="MARGLHITHWSSADLARQAVQDGIIDAISPRSIRRILHAVDLRP
                     HRTRFWKTARLDAQFMERAVRILWCYTYAGHLARRGWWVVCADELPNFQVLERVPIRR
                     AIPGSIEQREFEYKRHGTVTVLVFLVVHSGQMEAVCLEGKSAAHYTAALRHFRRRHRQ
                     LRGAYLIHDGDSTHTGDCTPGYLHEFRSWWRPRLLPPDASFLNQAELLLDAFELHYLK
                     RGSWRSREEFRAHIAAAWPEYNRLYAHPFEWEWTIPKMKQWFAKHGQ"
     gene            complement(1404..>1997)
                     /locus_tag="VHE33_13790"
     CDS             complement(1404..>1997)
                     /locus_tag="VHE33_13790"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HVT98569.1"
                     /translation="NMPPPGFTALFNGKDLTNWQGLIELPARNKMSPEQRAEAQKKAN
                     EKYLPHWTVQDGILQYDGKGQSLQTVKDYGDFELYVDWKIGPKGDSGIYLRGNPQVQI
                     WDNPVGSGGLYNNQKNPSKPLKVTDRPVGQWNTFHIIMKGDRVTVFLNAEKVVDNTPL
                     ENYWERGKPLPAKGPIELQHHGDRLWFKNIYVKELPN"
BASE COUNT          319 a          677 c          607 g          394 t
ORIGIN      
        1 ctagtggctc tggccaaaag tcctgcaaca aattaggggc agccgctatg ctggagatga
       61 agcagcgctt ccacttctcc tatcgagagg ctgcccccat gcgcggtcgc aaacctcgct
      121 cccttaccct cgcccccggc gacctcctct gcctccagcg catcgctcgc cagcccgcgc
      181 gccctttctt ccaggtccag cgcgctcgca tcgtcttggc cgtcgccgcg ggcgaaccga
      241 ttcaccgtat cgccgcccgc tgggagtgcg accgcgccac cgtctggcgc tgctgtcgcc
      301 gctaccagca gggcggcctc gctggactgt tggccgacgc cgaccgctcg gggcggcccg
      361 agcggatttc cccccctcca gcgcgcccag gtcatccaac tggcctgcct ggagccggtg
      421 gctcgggggc tgcacatcac ccattggtcc agcgccgacc tggcgcggca ggccgtgcag
      481 gacggcatca tcgatgccat cagcccgcgc agcatccgcc gcatcctgca tgcggttgac
      541 ctgcgaccgc accgcacgcg cttctggaag accgcacggc tggatgccca gttcatggag
      601 cgggccgtaa gaatcctttg gtgctacacc tacgccggcc acttggcccg ccgcggttgg
      661 tgggtggtct gtgccgacga gttgcccaat tttcaggtcc tggagcgcgt gccgatccgc
      721 cgggccatcc ccggctcgat cgagcagcgc gagttcgagt acaagcggca cggcaccgtg
      781 acggtgctgg tgttcctggt cgtccacagc gggcagatgg aggcggtgtg cctggagggc
      841 aagagcgccg cgcactacac cgcggcgctg cggcacttcc gccgccggca ccggcagctg
      901 cggggcgcgt acctgattca cgacggggac tccacccaca ccggcgactg cacgccgggc
      961 tacctgcatg agttccgcag ctggtggcgt ccgcggctgc tgccgcccga cgcctcgttc
     1021 ctgaaccaag cggagttgct gctcgatgcc tttgagctgc attacctgaa gcgagggtcg
     1081 tggcgcagcc gggaagaatt ccgggcgcac atcgcggcgg cgtggccgga gtacaaccgg
     1141 ctctacgccc acccgtttga gtgggagtgg acgatcccga agatgaagca atggttcgca
     1201 aagcatggcc aatgaatttg ttgcaggact tttggccagg gccactagcg agcgattcgc
     1261 cgccattcgc tgcctaaaag gcactaacat tttgatgaca agcccggagc gccagcgacg
     1321 ggtcgccgga acccgtcgct ggcgctccgg gcttgtgagt catcgcgtta gcgcctctaa
     1381 cgcgcatccc ggctaacact caatcagttc ggtaattcct tgacgtagat gttcttgaac
     1441 cacagcctgt cgccgtggtg ctgcagttcg atcggcccct tggccggcag cggtttgccg
     1501 cgctcccagt aattctcgag cggcgtattg tccacgactt tctcggcgtt gaggaacacg
     1561 gtgacacgat cgcccttcat gatgatgtgg aaggtgttcc actgaccgac cggccgatct
     1621 gtcaccttga gcggcttgct ggggttcttc tgattgttgt acagcccgcc cgaaccgacg
     1681 gggttgtccc agatttgcac ctgcggattg ccgcgcaggt agataccgct atcgcccttg
     1741 ggaccgatct tccaatcgac atacagctcg aaatcgccgt aatccttgac ggtttgcaag
     1801 ctctgcccct tgccatcgta ttgcaggatg ccatcctgca ccgtccagtg cggcagatat
     1861 ttctcgttgg ccttcttctg cgcctcggcc cgctgctccg gactcatctt gttccgcgcc
     1921 ggcaactcga tcagtccttg ccagttggtc aaatccttgc cgttgaacag cgcggtgaaa
     1981 cccggcggcg gcatgtt
//
LOCUS       DASZYN010000539         1553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_603690, whole genome shotgun sequence.
ACCESSION   DASZYN010000539 DASZYN010000000
VERSION     DASZYN010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603690"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VHE33_13795"
     CDS             <1..508
                     /locus_tag="VHE33_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98570.1"
                     /translation="NAVYGDFSMGQPVDCCVGGFHTAYVARQTANRIFVQTVVFATSL
                     DSAVANTVFGDPGEFADINALSHELAETVNDPFVNNVTPSYQVPGAPDGVCQNTLEVG
                     DITESFVPDYTDVTLHGFTYHPQTMGVLQWFQDNPHSTAINGDFSFPDSSLLQTQFAP
                     CAGVVPIG"
     gene            complement(617..1024)
                     /locus_tag="VHE33_13800"
     CDS             complement(617..1024)
                     /locus_tag="VHE33_13800"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HVT98571.1"
                     /translation="MATFAWSDAYSVKVQQFDAQHQKLFEIINSLADAMRAGQGEDVI
                     REVVGQLAVYTRTHFLQEEVAMRQAGYPDLPAHQAQHSKLMAEVEKHKKELDEGRKPN
                     LVAVLNFLRDWLVQHIQKSDKKYSDHLNAHGMR"
     gene            complement(1104..>1553)
                     /locus_tag="VHE33_13805"
     CDS             complement(1104..>1553)
                     /locus_tag="VHE33_13805"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT98572.1"
                     /translation="GRYETAPLEKLTVEQWDAMFETNTRAPFLAAQAAYPHLKDARGR
                     IINIGSLGGIHPWPTHAHYCTSKAALHMLTQTMSKAWAPHISVNCVAPGMIVNGEVDP
                     ELEHFARRTPMQRNGTSADVAAAVLFFATSPHFITGQILSVDGGLGL"
BASE COUNT          280 a          397 c          547 g          329 t
ORIGIN      
        1 caatgcagtc tacggcgact tttcgatggg gcagccggtg gactgctgtg tgggcggatt
       61 ccacacggcg tacgtagcgc ggcagacagc gaaccggatc tttgtgcaga cggtggtctt
      121 tgcgacatcg ctcgattccg cggttgcgaa tacggtcttc ggcgatccgg gcgaatttgc
      181 ggacattaac gcgctgtcgc acgaactggc ggagacggtg aacgacccgt ttgtgaacaa
      241 cgtcacgccg agttatcagg ttccgggagc gcccgacggg gtgtgccaga acactctcga
      301 ggtgggcgac atcacggaaa gctttgttcc ggactacacc gatgtgacgc tgcacgggtt
      361 cacgtatcat ccgcagacga tgggcgtgct gcagtggttc caggacaatc cgcattcaac
      421 ggcgatcaac ggggacttca gcttcccgga ttcgtcgctg ctgcagacgc agttcgcgcc
      481 gtgcgcaggg gttgttccga tcggttgatg aacagaggtc gctaaaaacc ggtcagcgtg
      541 catcgagcag agacaaaagg cgttcctttt cggaacgcct ttttcttgtc acgggcggga
      601 tacggcgtgc gcgggctcag cgcatgccgt gggcgttcag gtgatcggag tatttcttgt
      661 ccgatttctg gatgtgctgt accagccagt cgcgaagaaa attgagaacg gcgacaagat
      721 tgggcttgcg accttcgtcc agttccttct tgtgcttttc gacttcggcc atgagcttac
      781 tatgctgggc ctgatgggcg ggcagatcgg gatagcccgc ctggcgcatc gccacttctt
      841 cctggaggaa gtgggtgcgg gtatagacgg cgagttgacc gacgacctcg cggatgacgt
      901 cttcgccctg accggcacgc atggcatccg caagcgagtt gatgatttcg aagagtttct
      961 ggtgctgggc gtcaaactgc tgaaccttga cgctgtaggc atcgctccat gcaaacgtgg
     1021 ccatcggctg tctcccgatg agtctatcgg cagggcggac gaaaggatga gtctgaaggc
     1081 tggacaggat agccgttcgc ggatcagagg ccgaggccac cgtcgacgga gaggatctgg
     1141 ccggtgatga aatgggggct ggtggcgaag aagagcacag cggcggcgac gtcggcggag
     1201 gtgccgttgc gctgcatcgg ggtgcggcgg gcgaaatgct ccaattccgg atccacttct
     1261 ccgttgacga tcatgccggg ggcgacgcag ttgacgctga tgtggggggc ccaggctttg
     1321 gacatggtct gggtgagcat gtggagcgcg gctttggagg tgcagtagtg ggcgtgcgtg
     1381 ggccaggggt ggatgccgcc cagggatcct atattaatga tgcggccgcg agcgtctttg
     1441 aggtgggggt aagcggcctg ggcggcgagg aacggggcgc gggtgttggt ttcgaacatg
     1501 gcgtcccact gctctacggt gagcttttcc agcggcgcgg tttcgtatcg gcc
//
LOCUS       DASZYN010000540         6345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1121748, whole genome shotgun sequence.
ACCESSION   DASZYN010000540 DASZYN010000000
VERSION     DASZYN010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121748"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..2101)
                     /gene="uvrB"
                     /locus_tag="VHE33_13810"
     CDS             complement(113..2101)
                     /gene="uvrB"
                     /locus_tag="VHE33_13810"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HVT98573.1"
                     /translation="MDFQLVSDYKPRGDQGRAIEELVTGIEAGEKHQVLLGVTGSGKT
                     FTVAKMIEHLNRPSLVLAHNKTLAAQLFHEFKQFFPNNAVEYFVSYYDYYQPEAYIPS
                     GDLYIEKEATINEELDKLRLSATRSLFERRDCIIVASVSCIYGLGSPEAYYGMLLLLE
                     KGQRIRRDDITRRLVEILYERNDADFRRGTFRVRGDVIEIFPTYDENAYRIELFGDEI
                     DSLSQIDPLFGTVRQKYARLPIYPKSHYVVQPERRKTAIETILDELGWWEKELENQGR
                     MVESQRVHQRTRFDLEMIKSVGYCHGIENYSRHFSGRLPGEPPPTLLDYFPRDYLLFI
                     DESHVTVPQLHGMWHGDRSRKQNLVDYGFRLPSAMDNRPLKFEEFESRTNQLVYVSAT
                     PGPYELTKAAGVVVEQIIRPTGLVDPEVEIRPVRGQIDDLLHEIRDRAAKNERVLVTT
                     LTKRMAEDLAGYYSEVGVRCRYMHSEIETLERVKLLRDLRKGEYDVLIGINLLREGLD
                     LPEVSLVAILDADKEGFLRSSGSLIQTIGRAARHLNGRAILYADKMTDSMKQAIGETD
                     RRRTVQRAYNEENGIVPQSIVSHIDMSLAKILQAEYADLAEEAEEMPELQSQEELDSY
                     IAKLEAEMREAAKRFEFEKAAKLRDTIKELRTKEFLFK"
     gene            complement(2168..2539)
                     /locus_tag="VHE33_13815"
     CDS             complement(2168..2539)
                     /locus_tag="VHE33_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98574.1"
                     /translation="MNVLRSMAAVLLSYIVVTVIVILSDPLLAHIFPGQYVPGKTVPA
                     SILLIGAAIFAVASILGGWLCVRIAPAKPGKHLLALFILGEVAGVISTILNWNKWPHW
                     NSFLWLVLWPICIWIGGRGRK"
     gene            complement(2667..4901)
                     /gene="katG"
                     /locus_tag="VHE33_13820"
     CDS             complement(2667..4901)
                     /gene="katG"
                     /locus_tag="VHE33_13820"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011635.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HVT98575.1"
                     /translation="MSAEAKCPFPHGNGHATRSNSDWWPNALNLNILHQHSSLSDPMG
                     EEFDYTEEFKSLDYAGLKKDLEALMTDSQPWWPADFGHYGGLFIRMAWHSAGTYRTLD
                     GRGGGGRGQQRFAPLNSWPDNVNLDKARRLLWPIKQKYGRKISWADLMILAGNVALES
                     MGFKTFGFAGGRADVWEPDMDVNWGAEETWLGTDKRYSGERDLAKPFGATTMGLIYVN
                     PEGPEGKPDPIAAAKDIRETFGRMAMNDEETVALIAGGHTFGKTHGAGPASYVGKEPE
                     GTEITEQGIGWSSTYLSGIAGDAITSGLEVTWSNTPTKWSNHFFDNLFKYEWELTKSP
                     GGAHQWKPKGNAGEGTVPDAHDSGKHHAPGMLTTDLSLRFDPIYEKISRRFYEHPDEF
                     ADAFARAWFKLTHRDMGPRTRYLGPEVPAEELIWQDPIPAVDHPLVDEKDIASLKTKI
                     IASGLTISQLVSTAWALASTYRGSDKRGGANGARIRLAPQKDWEVNEPEQLQKVLKTL
                     EGIQSEFNKSQAGGKKVSLADLIVLAGCAGVEQAAKNAGVNVTVPFAPGRMDASAEQT
                     DAASFAVLEPSADGFRNYNSGSNQVPPEAALLDRAQLLTLTAPEMTVLVGGMRVLNTN
                     VRQTRHGVLTKQPEALTNEYFSNLLDMGTVWKATSDAQDVFEGRDRKTGEVKWTATRV
                     DLVFGSNSQLRALAEVYASSDAKKKFADDFVAVWNKVMNLDRFDLWLKRPHSVKLEEP
                     VGAN"
     gene            5066..5959
                     /locus_tag="VHE33_13825"
     CDS             5066..5959
                     /locus_tag="VHE33_13825"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HVT98576.1"
                     /translation="MEVHQLRYVCAIAETGSFSRAAERCQVTQPSLSQQISKLEDELG
                     TRLFDRLARRVRLTDAGRTFLPHARTILHETELARSEVSGRRKDARGTVIVGVIPTIA
                     PYDLPQRITKFMHRFPDASLRVVEETTPVLLESLRSLAIDIAILSLPLRQREFETTAL
                     LTERLYVALPPDHPRAGAPAIALNELRNERFVMLRDSHCFREISLAACHRAHLDPRIA
                     FESNQIGSLLGMVAAGVGIALVPEMAVDRGGGCCYVRISDPKAVRTIAAARLKGRSFS
                     RVQEAFVEFPKRPARQRTRKP"
     gene            complement(6026..>6345)
                     /locus_tag="VHE33_13830"
     CDS             complement(6026..>6345)
                     /locus_tag="VHE33_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98577.1"
                     /translation="ALVLGLALCGGTALAVHAQEGSPWQPVEPTGSWSHAWHSGFHDG
                     IEAARHDIDAHRRPDPGRHDTFRHPDLPREERNDFREGFRRGYDMAYDHAGHHHDHDD
                     FPR"
BASE COUNT         1259 a         1910 c         1834 g         1342 t
ORIGIN      
        1 gaactcactg atcgggaccg caatcatctt gctgggggtt ccgctcttcc ttgtattcag
       61 aaaacaggcc cgaacctcgg cagccggcgg ttccgtccga tagcccctcg attcacttga
      121 acaagaattc cttcgtccgc agctccttga tggtgtcgcg cagcttcgcc gccttctcga
      181 attcgaatct ctttgccgct tcgcgcattt ccgcttcgag cttcgcgatg taactgtcga
      241 gttcttcctg tgactgcaac tcgggcatct cctccgcctc ttccgccagg tcggcatact
      301 ctgcctgaag aatcttcgcc agagacatgt cgatgtggct gacgatcgac tgaggcacga
      361 tgccgttttc ctcgttatag gcacgctgca cagttcgccg gcgatcagtt tcgccgatgg
      421 cctgcttcat ggaatccgtc atcttatccg cgtagagaat ggcgcggccg ttgagatggc
      481 gagcggcacg cccgatggtc tggatcagcg atcccgacga acgcaggaat ccttctttgt
      541 cggcatcgag aattgcaacg agcgagactt cgggcaggtc gaggccttcc cgcagcaggt
      601 tgatgccgat gagcacgtcg tactcgcctt tgcgcagatc gcggagcagc ttcacccgtt
      661 ccagcgtctc gatttcggag tgcatgtagc ggcagcgcac gcccacttcg gaatagtagc
      721 cggctaggtc ctcggccatg cgcttggtga gcgtggtgac cagcacgcgc tcgttctttg
      781 cggcgcggtc gcggatctcg tgcagcagat cgtcgatctg tccacgcacc ggacggatct
      841 ccacctcggg atcgaccaat cccgtcggcc gaataatctg ttccacgaca accccggcag
      901 ctttcgtcag ctcgtagggt ccgggcgtcg ctgaaacgta gacgagctgg ttggtacgcg
      961 actcaaactc ttcaaacttg agcggacggt tgtccatggc cgaaggaaga cggaacccgt
     1021 aatcaaccag attctgtttg cgcgaacgat cgccgtgcca catgccatgc agctgcggca
     1081 cggtaacgtg cgattcatcg atgaagagca ggtagtcgcg cgggaagtag tcgagcagcg
     1141 tcggcggcgg ctcgcccggc agccggccgg agaagtggcg cgaataattc tcgatgccat
     1201 ggcaatagcc gacggacttg atcatttcca gatcgaagcg ggttcgctgg tggacgcgct
     1261 gcgactccac catgcgtccc tggttctcga gctccttttc ccaccagccg agttcgtcaa
     1321 ggatcgtctc gatcgccgtc ttgcgccgct ccggctgcac tacgtaatgg ctcttgggat
     1381 agatgggcag gcgcgcgtac ttctgacgca cggtgccgaa caggggatcg atttgcgaaa
     1441 gagaatcgat ctcatcgcca aagagctcga tgcggtaagc gttctcgtca taagtcggaa
     1501 agatctcaat cacatcgcca cgaacccgaa aggttccgcg gcgaaagtcc gcgtcgttgc
     1561 gctcgtagag aatttcgacc aggcggcgcg tgatgtcgtc acgccgaatg cgctgccctt
     1621 tctccagcag cagcagcatg ccgtaatatg cttccggcga gccgagtccg tagatgcagg
     1681 aaacggacgc gacgatgatg cagtcgcgac gctcgaagag cgagcgcgta gccgacaggc
     1741 ggagcttgtc cagctcctcg ttgatggtgg cttccttctc gatgtacagg tcgccggagg
     1801 gaatgtaagc ctcgggctgg tagtagtcgt agtaggagac gaagtactcg acggcgttat
     1861 tgggaaagaa ctgcttgaac tcatgaaaaa gctgggcggc aagggtcttg ttgtgcgcca
     1921 gcaccagcga agggcgattg agatgctcga tcatcttcgc cactgtgaag gtttttcctg
     1981 agccagtgac accgagcaga acctgatgct tctcacctgc ctcaatgccg gtgacgagtt
     2041 cctcgatagc gcggccctga tcgccacgcg gcttgtaatc gctaacgagc tggaagtcca
     2101 cagatcgatt gtaggcgatg gaaaggcgaa gacctgtgcg ctccgtggaa aaaccaggcg
     2161 tagcctttca tttcctgccg cgtccaccga tccagatgca aatgggccag agcacaagcc
     2221 aaagaaagga attccagtgc ggccacttat tccagttgag gatggttgag atgacgccgg
     2281 cgacttcacc caggatgaaa agcgcgagga ggtgtttgcc cggctttgca ggcgcaatgc
     2341 gcacgcaaag ccagccgccg aggatggagg cgaccgcaaa gatggccgca ccgatcagca
     2401 ggatggaggc gggcaccgtc ttgcccggga cgtactggcc cggaaagata tgggctagca
     2461 agggatcact gaggatcacg atgacggtga cgacgatgta gctcaagagg acagctgcca
     2521 tcgagcgaag cacattcatg cccacgtctc ctggatgacg atgtctgatt cggggagaga
     2581 tgcaggatag cgcgagccag accgcaacgc aaaagaaact gcccgcactc tttgggcgag
     2641 tgcgggcatg aatgaaaccg ccgagcttag ttggcgccga cgggctcctc cagtttcaca
     2701 ctgtgcggcc gcttcaacca gaggtcaaaa cgatcaaggt tcatcacctt attccagacc
     2761 gccacgaagt cgtcggcgaa tttcttcttc gcatccgaac tcgcgtagac ctccgccaga
     2821 gcccgcagct gagaattcga accgaagacc agatcaaccc gtgttgccgt ccacttcact
     2881 tctccggtct tccgatcgcg tccttcgaag acgtcctgcg catcggaggt cgccttccac
     2941 accgttccca tgtcgagcag gttgctaaag tattcgtttg tcagcgcctc cggctgtttc
     3001 gtcaggacac catggcgcgt ctgccgaaca ttcgtattca gcacgcgcat gccacccacg
     3061 agcactgtca tttcgggtgc cgtcaacgtg agcaattgcg ctctgtccag cagcgccgcc
     3121 tccggcggta cttggttgct gccactgttg taattgcgga agccatcggc ggacggctca
     3181 aggacggcga aggaggccgc atcggtttgt tcggccgaag cgtccatgcg tcccggagca
     3241 aagggaaccg tgacgttcac gccggcattc ttcgcagcct gctccactcc agcgcaaccg
     3301 gcgagaacga tcagatcggc cagcgacact ttcttgccgc ccgcctggct cttattgaac
     3361 tcgctctgaa ttccctccag tgtcttcaac accttctgca gttgttcggg ctcgttcacc
     3421 tcccagtcct tttgcggagc gagccggatc cgtgcaccat tcgcgccccc acgcttatcg
     3481 gaacctcggt aggtggaagc caatgcccaa gcggttgaaa ccagctgcga aatcgtcagc
     3541 ccggaagcga tgattttcgt tttcagcgag gcgatgtcct tttcgtccac caacggatga
     3601 tcgactgccg ggatgggatc ctgccaaatg agctcttctg ccggcacttc cggaccgaga
     3661 tagcgcgtgc gcgggcccat atctcgatgc gtcagcttga accaggcacg cgcaaatgca
     3721 tcggcaaatt cgtcggggtg ctcgtaaaat cgccgcgaga tcttctcgta aattgggtcg
     3781 aagcgcagcg acagatcggt ggttagcatg cctggagcgt gatgctttcc agaatcgtgc
     3841 gcgtcgggca ccgtgccttc acccgcattc cccttcggct tccactggtg ggcacctccc
     3901 ggactcttcg tcagttccca ctcatatttg aaaagattgt cgaagaaatg attgctccat
     3961 ttcgtcgggg tattgctcca cgtcacttcc aggccgctcg tgatcgcgtc accggcaatg
     4021 cccgaaaggt acgtgctgga ccaaccaatt ccctgctccg taatctccgt gccctctggc
     4081 tccttgccaa cgtagcttgc cggaccggcg ccatgcgtct tgccaaatgt atggccgccc
     4141 gcaatcagag ctacggtctc ctcatcgttc atcgccattc ggccaaaggt ctctcgaata
     4201 tcctttgcgg ccgcaatcgg atctggtttt ccttccggcc cttccggatt gacgtagatc
     4261 agtcccatgg tcgtggcgcc aaacggctta gcaaggtcgc gctcgccgga gtagcgcttg
     4321 tctgttccca gccaagtctc ttctgctccc cagttcacgt ccatatccgg ttcccaaacg
     4381 tccgcgcggc cgccggcgaa tccaaaagtt ttaaagccca tcgattccag agcaacgttg
     4441 ccggccagaa tcatcagatc ggcccatgag atttttcgtc catacttctg cttgatcggc
     4501 cacagcaagc ggcgagcctt atccagattc acattgtcgg gccagctgtt caggggcgcg
     4561 aaacgctgct gccccctgcc tccgcctcca cggccgtcca gggtgcggta ggttcctgcg
     4621 ctgtgccatg ccatccgaat aaaaagcccg ccgtagtgcc cgaagtcggc cggccaccag
     4681 ggctgggaat ccgtcatcag cgcttcaaga tccttcttca atccggcata gtcgaggctt
     4741 ttgaattctt ccgtgtaatc gaactcctcg cccatgggat cggaaaggga ggaatgctgg
     4801 tgcaggatat tcaggttcaa tgcgttgggc caccagtcgc tgttcgaacg ggtagcgtgg
     4861 ccgttgccgt gtggaaacgg acatttcgct tcggctgaca ttctttgtct ccttctgctt
     4921 ttttgggtcc ggcgatcggg ttccctgtgg agcccgcgta tcgatcgatg agagcggctt
     4981 ccgtttgtcc ggaaacatcg aatgtagcac catggagacg catagttcca atacataacg
     5041 tgcatcatcg gtataggatc cacctatgga ggttcatcag ctgcgctacg tttgcgcgat
     5101 cgccgaaacc ggcagtttta gccgggccgc cgagcgctgt caggtcaccc aaccgtcgct
     5161 ttctcagcag atttccaaac tggaagacga actcggcacg aggctgttcg atcggctcgc
     5221 tcgccgcgta cgactcaccg atgcggggcg gacctttctg ccgcatgctc gcacgattct
     5281 gcacgagacc gagctggcgc ggagcgaagt gtcgggacgg cgtaaagacg cgcgggggac
     5341 ggtcattgtc ggggtcatcc ccaccattgc accgtacgat ttgccgcagc ggatcacgaa
     5401 gttcatgcac cggtttcctg acgcaagtct gcgtgtggtt gaagaaacca cccccgtgct
     5461 gctggagagc ctgcgcagtt tggcgattga cattgcgatc ctgtctctac cgctgcgtca
     5521 acgcgaattt gaaacaacgg ctctgctcac ggagcgcctg tacgtggccc tgcctccgga
     5581 tcaccccaga gccggggcgc ccgccatcgc cctgaacgag cttcgcaacg agcgcttcgt
     5641 gatgctacgc gacagccatt gctttcgtga aatctcactc gccgcctgtc accgcgcgca
     5701 tctggatccg cgcatcgcat tcgagagtaa tcagatcggc agcctgctgg gaatggttgc
     5761 ggcaggagtc ggaatcgctt tggtcccgga aatggcggtg gatcgaggcg gaggctgctg
     5821 ctacgtacgc atctccgatc ccaaggctgt gcgcacgatt gcggcagctc gattgaaagg
     5881 aagatcgttc agccgcgtac aggaagcctt tgttgaattc cccaaaaggc ccgccagaca
     5941 gcgaacccgg aagccgtgac gcgcctgtaa agaaaaaggg agccggcttg aatgccggct
     6001 cccttgccgc cccgaattca gcggactaac ggggaaaatc gtcgtggtcg tgatggtgtc
     6061 cggcatggtc atacgccatg tcgtagccgc ggcgaaagcc ttctcggaaa tcgtttcgtt
     6121 cttcgcgggg caaatcggga tggcggaatg tgtcgtggcg gcccggatcc ggccggcgat
     6181 gcgcgtcaat gtcgtgacgc gcggcttcaa tgccatcgtg gaaaccactg tgccatgcat
     6241 gcgaccagct tcccgtaggt tcgacaggtt gccagggcga gccctcctgc gcatgaacag
     6301 ccagcgcagt gccgccacat aacgccaaac ccagtacgag agcgg
//
LOCUS       DASZYN010000541         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_795622, whole genome shotgun sequence.
ACCESSION   DASZYN010000541 DASZYN010000000
VERSION     DASZYN010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795622"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..500)
                     /locus_tag="VHE33_13835"
     CDS             complement(219..500)
                     /locus_tag="VHE33_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98578.1"
                     /translation="MEPVHIVVQHVRRESRECFRLVAYRGVEGFWPVEFTSLDDLVQA
                     LRIVLPAFDRTQIAVAGPSEESSILWAGDLKLSDAELARIGLRKIHPRV"
     gene            616..954
                     /locus_tag="VHE33_13840"
     CDS             616..954
                     /locus_tag="VHE33_13840"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVT98579.1"
                     /translation="MPDSLRQFKAEIFQALAHPTRIAIVDLLREGELTAGTFSDRLGL
                     EQANLSQHLAVLRARQIVVARKAGNQVFYSVRDPLLWKVLDLLRRYFQKQVGESMALL
                     EELEKAEERR"
     gene            954..>2020
                     /locus_tag="VHE33_13845"
     CDS             954..>2020
                     /locus_tag="VHE33_13845"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HVT98580.1"
                     /translation="MALPFPQQWLPRSVTSLRNYSVRRFSHDLASGVTVGLVALPLAM
                     AFGIASGVTPQAGLYTAVVAGFLISALGGSRVQIGGPTGAFVVIVAGIVARFGLSGLT
                     LVTLMAGAMLVVMGATGLGTAVKFIPRPVIIGFTNGIALLIASTQIKDFLGLRTGAVP
                     SAFLPRMKVLVQNIGTLQWPALAVAVATLAIILLWPRVSRRIPGSILAVLFCTAASTL
                     LHLHVETIGTRFGGIPKGFPPFHLPALHAEHILPLLPSALTVALLGAIESLLSAVVAD
                     GMAGDRHNSNVELVAQGVANLFSPLFGGIPATGAIARTATNIRSGALTPVSGMVHALT
                     LLTILLVAAPLARFIPLATLAA"
BASE COUNT          328 a          593 c          661 g          438 t
ORIGIN      
        1 aacggcatcg gactcggtga ttcaggagtc ccaggccgcc gtttagcact ttcgccctat
       61 gactgctaac gtagcgggga tgcctgtgga tgtcaagtgg ggtcaggtaa aaagtgagtg
      121 aaagacactc aggtaatcag acagggtcga ttcgcggcgg gactgcgggg gttcgaggtt
      181 gggggtgatc cgggtgtaag cgtttcattg tacgtggctt aaacgcgcgg atggattttt
      241 ctcaatccga tccttgctaa ctcggcatca ctcagcttta gatcgccggc ccataggatc
      301 gaggactctt cgcttgggcc ggccacggcg atctgggttc ggtcgaaggc cggcaggacg
      361 atacgaagcg cctgcaccag atcatcgagc gaagtgaatt cgacgggcca gaagccttca
      421 acgccacgat atgcgacaag acgaaagcat tctcggctct ctctgcgcac atgctgcaca
      481 acgatgtgga ccggctccat ctggggacag actccgcaaa tcgactcgcg acccacatcc
      541 atccgtaccg ggccggcgct ccgggagcga aggatcgggg gagtcgtatt cgaaacttcg
      601 aatataataa agtacatgcc cgactcgctg cgacagttta aggcggaaat ttttcaggct
      661 ctggctcacc ccacccggat cgccattgtg gatctgctgc gcgaggggga gctgacggcg
      721 gggacgtttt ccgatcgtct tggtcttgag caggcgaatc tgtcccagca tctggccgtg
      781 ctgcgggcgc ggcagatcgt ggtcgcgcgc aaggccggca accaggtgtt ttattcggtg
      841 cgcgatcccc tgctgtggaa ggtgctcgac ctgctgcgcc gctactttca gaagcaggtg
      901 ggcgaatcga tggccctgct ggaagaactg gagaaagccg aggaacggcg ttagtggcgc
      961 tgccctttcc ccagcagtgg ctccctcgat cggtcacttc ccttcgcaac tattcggtgc
     1021 ggcgcttcag ccatgatctg gcttcgggcg tcaccgttgg actggtggcc ctgccgctgg
     1081 cgatggcctt cggcattgcg tccggcgtaa ctccacaggc cgggctttat acggcggtgg
     1141 tggccggatt tctgatctcg gcgctgggcg ggtcgcgcgt acagattggt ggcccgacgg
     1201 gggctttcgt ggtgatcgtc gccggcatcg tggcgcgctt cggcctttcg gggctgacgc
     1261 tggtgacgct gatggctgga gcgatgctgg tggtgatggg ggccacgggg ctgggaacgg
     1321 cggtcaaatt cattccgcgg ccggtgatca tcgggttcac gaatggcatt gcgctgctga
     1381 ttgcctccac gcagatcaaa gactttctgg ggctgcgcac cggtgctgtg cccagtgctt
     1441 ttctgccgcg catgaaggtt cttgtccaga acatcgggac tcttcagtgg cctgcgctgg
     1501 cggttgcggt cgccacgctg gcgatcatcc tgctctggcc gcgggtgagc cgtcgcatcc
     1561 ccggttccat cctggcagtt ctgttctgca ccgccgcttc taccctgctg catctgcacg
     1621 tggagacgat cggcacgcgt ttcgggggga tacccaaagg atttccgccc ttccatctgc
     1681 ctgcgctcca tgccgagcac atcctgccgc tgctgccatc cgcgttgacg gtggcgctgc
     1741 tgggcgcgat tgaaagcctg ctctccgcgg tggtcgcgga tgggatggca ggcgatcggc
     1801 acaactccaa cgtggagctg gtggctcagg gagtggcgaa tctcttctct ccactctttg
     1861 gcggcattcc tgcaaccggc gctatcgcgc gcacggcgac gaatatccgt tccggcgcgc
     1921 tgacaccggt ttcggggatg gtgcatgcgc tcacgctgct gacgatcctg ctggtcgcgg
     1981 ccccgctggc ccgcttcatt ccgctggcta cgctcgcggc
//
LOCUS       DASZYN010000542         4783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_475158, whole genome shotgun sequence.
ACCESSION   DASZYN010000542 DASZYN010000000
VERSION     DASZYN010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475158"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..734
                     /locus_tag="VHE33_13850"
     CDS             303..734
                     /locus_tag="VHE33_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98581.1"
                     /translation="MKRLSLFAVQFLAAALVGSVCGPSLRAQSDIAITASVSHAFTIG
                     TQTLGAGTYRFSQGSDAFLLSVINVKTGEQKLFTVLPEQHGDFAQQGYLRFRTVGESS
                     VLSEIHFSGTDVFSAVVQKQKPERRLAKKSSAKDTVSVAQR"
     gene            808..1281
                     /locus_tag="VHE33_13855"
     CDS             808..1281
                     /locus_tag="VHE33_13855"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HVT98582.1"
                     /translation="MKRIILWSSVGVLLLAMTFVVFRVHAGIRRGWGGHGWHRGGPMS
                     YVAHELKLSSDQRAQIRALWEAERPGVATHVHELLAENKEMNAATVQEKPDPTKVKEI
                     ADREAATIAALLVEKQQLQAKIYTTVLNPEQRAKADAWEKKWESRLDRAAERLGK"
     gene            complement(1298..2761)
                     /locus_tag="VHE33_13860"
     CDS             complement(1298..2761)
                     /locus_tag="VHE33_13860"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HVT98583.1"
                     /translation="MVSFVHLANDGVGNVVDGRFPLLRWLGGTDSSSVFLTEFEGDSP
                     RKAAIKLIPAAGIDADARVAQWVTAKNLSHPHLLRVFHAGRCAIDGEDYIYVVTEYAD
                     EVLSEILAARPLSPEETAEMLSPVLDALSWLHARDLVHGHLHPSNILVVDESLKISID
                     RLHNDGEFGRPALLSGQFDAPEMATGLLSPATDIWSVGVVVVQCLFRHPLAWNGPRGQ
                     EPVVPATIPEPFFSIARRCLHVDPARRSTLDDIRTALDPAKVAKPAPAKTAAKASSPR
                     HIPTSVLAGAAVVLGGVIGAFIAFSHHNQPPSAESQMPAAQTSEPAPAAVEPQPPATE
                     ATSPQASTPKAAAPEPPPPQTQIPASPQQESPQSASPAPSAPAPDAAPLQHSGATGAV
                     ASRPTPDIPQHILDTVHGHVRVRVRVDVDAQGKITNAVLDDPGPSRYFADKALATARN
                     WTFTPAHSNGQAVPSTWMLHFSFGHDGSTVTSTETAP"
     gene            2921..3706
                     /locus_tag="VHE33_13865"
     CDS             2921..3706
                     /locus_tag="VHE33_13865"
                     /inference="COORDINATES: protein motif:HMM:NF006384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT98584.1"
                     /translation="MDLGLHDSVIVVTGGGAGIGQAISRACLEEGATVVVVSRASDNV
                     KNFLAEMLNTRRKCDFVEAHLADPAQCRKAVDYVRDRYGKLLGLVNNAGANDGVGLES
                     GSPEKFMESLKQNLVHYYAMAHYALPMLKASHGSIVNISSKVALTGQGGTSAYAAAKG
                     AQLALTREWAAELAKDGVRANSVLPAEVMTPLYERWISSRPDPQAALNTIAGRIPLGH
                     RFTEAAEIAAMTVFLLSPTQSGHTTGQNIVVDGGYTHLDRALT"
     gene            complement(3931..4365)
                     /locus_tag="VHE33_13870"
     CDS             complement(3931..4365)
                     /locus_tag="VHE33_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98585.1"
                     /translation="MNVQQQINDYLATQPEPKRSEMRQLHHTILALMPACKLWFLDGK
                     DEKGKTVSNPNIGYGSQLMKYADGKTKEFYQIGISANTGGISVYIMGLNDKKYLANTF
                     GKKLGKATVTGYCIKFRKLTDINIDGLNAAIQSGIEQTGIQH"
     gene            complement(4417..>4783)
                     /locus_tag="VHE33_13875"
     CDS             complement(4417..>4783)
                     /locus_tag="VHE33_13875"
                     /inference="COORDINATES: protein motif:HMM:NF020119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase C-terminal
                     non-catalytic domain-containing protein"
                     /protein_id="HVT98586.1"
                     /translation="LIDVYPDEVAAQPDMGGYQLMISADIFRGRYRESFTVPHPIQPN
                     TPLDYTFNLPPANHVFLPGHRIMVQIQSSWFPLYDRNPQTYVDNIFWAKSADYQKAEQ
                     KIWHAPGHQSYIDLPLVKQ"
BASE COUNT          914 a         1187 c         1697 g          985 t
ORIGIN      
        1 ccgcgggatc cgtcgtgcac cgtatccatg gtgaagcctt cggtcgatag gcgctcggcg
       61 agaagcttgc agagctgcac atcatcgtcg acgagaagaa cccggtccat gctgcttcgc
      121 cacctgtccc agacccgatg ctagtcgatc cgagtgggcg ggtcgcagtc tttacacaac
      181 tttacttagc ttcaccaacc tatatcgcac ttgcttcggg cgactgctgg aatcgggatg
      241 tggcgagtgg ctttcaggaa ctcggcacgc aagcgaatcc agcactttga aatggagaaa
      301 cgatgaagcg cctgagttta tttgcggttc aattcctggc ggcggcgctg gtgggcagcg
      361 tttgcgggcc gagcctgcgc gcccagagcg acatagcgat cactgcgagt gtttcgcacg
      421 cgttcacgat cggaacgcaa acccttggag ctggaaccta tcgcttcagc cagggatcgg
      481 atgcgttttt gctttcggtg atcaatgtga agaccggcga gcagaagctg ttcacggtgc
      541 tcccggagca gcatggcgac tttgcccagc aggggtattt gcggtttcgc acggtgggag
      601 agagcagcgt actgagcgaa atccattttt cgggaacgga tgtttttagt gctgtggttc
      661 agaagcagaa gccggagaga cggctggcaa agaagtcttc ggcgaaggac acggtgtcgg
      721 ttgcccagag gtgaggacga ccgaggcggc ggctgttttg aaccgagcga ttggtgagtc
      781 gatttgagag aaatcggagg agtttgtatg aagcgaatca ttctttggtc cagcgttggg
      841 gttttgttgc tggccatgac gtttgtcgtt ttccgcgtgc atgcgggcat ccggcgcggc
      901 tggggcgggc acggatggca tcggggtggt ccgatgagct atgtggcgca cgaattgaag
      961 ctgagttccg atcaacgggc gcagattcgg gcgctttggg aggcggagcg accgggtgtt
     1021 gccacgcatg ttcacgaact cctggccgaa aacaaggaga tgaacgccgc cacggtgcag
     1081 gagaagcctg atccgacgaa ggtgaaagag attgccgatc gggaggctgc gacgatcgcg
     1141 gctctgctgg tggagaagca acagctacag gcgaagatct atacgacggt tctgaatccg
     1201 gaacagcggg cgaaggccga cgcatgggag aagaagtggg agtcgcggct cgaccgggcg
     1261 gctgagcggc tcgggaaata accggcggag caacgagcta gggcgcggtt tcggtggagg
     1321 taacggtgct tccgtcgtga ccgaaggaga agtggagcat ccacgtgctg gggacggcct
     1381 ggccgttgct gtgtgcgggg gtgaaggtcc agttgcgcgc ggtagcgagt gccttatcgg
     1441 caaagtagcg gctgggaccg gggtcgtcga ggaccgcgtt ggtgatcttg ccctgtgcat
     1501 cgacatcaac gcggactcgc acgcgaacgt ggccgtggac tgtatccagg atgtgctggg
     1561 gtatatcggg agtggggcgg gaggcgaccg cgccggtcgc tccggaatgc tgaagaggcg
     1621 cagcgtctgg cgctggagca gacggtgccg gagacgctga ctgcggcgac tcctgctgcg
     1681 gagaggctgg gatctgggtc tgcggcggcg gaggttcggg cgctgcagcc tttggtgttg
     1741 acgcttgcgg ggacgtggcc tccgttgcag gaggctgcgg ctcgacggca gctggcgcgg
     1801 gctcggaggt ctgggctgcg ggcatctgcg attcggccga cggcggctga ttgtggtgag
     1861 aaaacgcgat gaaggcgcca atgacgccgc ccagaaccac ggctgcgcct gccagaactg
     1921 aggtggggat gtggcgcgga gatgatgctt ttgcggcggt cttcgcaggg gcgggtttgg
     1981 cgacttttgc gggatcgagg gcggtgcgga tgtcgtcgag ggtcgagcgg cgcgcggggt
     2041 ccacgtggag acagcgtcgc gcgatggaga agaagggctc gggaatggtg gcggggacga
     2101 cgggttcctg accgcgcggg ccattccagg ccagagggtg gcggaagagg cactgaacta
     2161 caacgacacc gaccgaccag atatcggtgg ctggggagag aagtccggta gccatctccg
     2221 gggcgtcaaa ctggccggag aggagcgccg ggcgaccgaa ctcgccgtcg ttgtgcaggc
     2281 gatcgatgga aatcttgagg ctttcgtcga cgaccaggat gttggaggga tggaggtggc
     2341 cgtggaccag gtcgcgggcg tggagccacg agagcgcgtc gagcacgggg gagagcatct
     2401 ccgcggtttc ctcggggctg agggggcggg cggcgaggat ctcggagagc acttcatcgg
     2461 catattcggt gacgacgtag atgtagtctt cgccgtcgat ggcgcagcgg ccggcatgga
     2521 acacgcgcag gagatgcggg tgcgagaggt tcttcgcggt gacccactgg gcgacgcgag
     2581 catcggcatc gatgccggcg gccggaatca gtttgatggc ggctttgcgg ggtgaatccc
     2641 cttcgaattc agtgaggaag acactgctgc tgtcggtgcc gcctaaccac cggaggaggg
     2701 ggaatctccc gtcgacgaca tttccgacgc catcgttggc gaggtgcaca aaactcacca
     2761 ttgaatccat tctcatctat atcggcagat cgcgcatccg ttcaagtgca tataacggcg
     2821 acatcacccc ggatggttcc tggcgtacct gtttggatgc cggatggcag tggtcccgcc
     2881 tgagggaaac gcggctggcc gggggcatta tccttcctgc atggatctgg gactgcatga
     2941 cagtgtgatt gtcgtaaccg gaggcggcgc gggcattggg caggcgatct cgcgcgcgtg
     3001 tctggaggaa ggcgcgacgg tggtagtggt gagccgcgcg tcggacaacg tgaagaactt
     3061 tctggccgag atgctgaaca cgcggcgcaa gtgcgacttc gtggaggcgc atctggcgga
     3121 ccccgcgcag tgcaggaagg cagtggacta cgtacgcgat cgctatggca agctgcttgg
     3181 cctggtgaac aacgcgggtg cgaacgatgg ggtgggactg gagtcaggat cgccggagaa
     3241 gttcatggag agcctgaagc agaacctcgt ccactactac gcgatggcgc attacgcgct
     3301 gccgatgttg aaggcatcgc acggatcgat tgtgaacatc agctcgaagg ttgcgctgac
     3361 cgggcagggt ggaacgtcgg cctacgcggc ggcgaagggc gcgcagctgg cgctgacgcg
     3421 cgagtgggcg gccgagctgg cgaaggatgg ggttcgggcc aactcggtgt tgccggccga
     3481 ggtgatgacg ccgctctatg agcggtggat ctcgtcgcga ccggatccac aggcagcgct
     3541 gaatacgatt gcggggcgga ttccgctggg gcatcggttt accgaggctg cggagatcgc
     3601 tgcgatgacg gtgtttttgc tatcgccgac ccagagcgga catacgacgg ggcagaacat
     3661 tgtggtcgat ggggggtata cgcatctgga tcgggccctg acgtagattg gacccagtaa
     3721 tccagggaga cccacgttcc agtctcttgc caggactgaa gccatggcga tgggtcgtat
     3781 tcctactcat ctcaacaccc gggatgcaac agcgatcgcg ctttgcgcga tgcccacatc
     3841 tccaactaca gagatgtggg gcacccggct tgcgaggaaa tgccaccggg ttccaggact
     3901 gaagcaatct cggatggacg taagtcgaga ttagtgctga atgcctgttt gttcgatgcc
     3961 ggattggatg gccgcattaa gtccatcgat gttgatatct gttagttttc tgaacttaat
     4021 gcaatatccc gtgacagttg ctttgccgag tttcttccca aaggtgttgg ccaggtactt
     4081 cttgtcattt aatcccataa tgtagacaga aatacctcca gtattcgcac tgataccgat
     4141 ttgatagaat tctttggttt ttccatcggc atatttcatg agctgcgatc cgtatccaat
     4201 gtttggattg gaaacggttt tgcctttctc atcctttccg tccaggaacc ataacttaca
     4261 tgccggcatc aacgccagaa tggtgtgatg cagctgccgc atttcgctgc gttttggttc
     4321 tggctgcgtg gctagatagt cgttaatttg ttgctgcaca ttcatgtcaa tctccgcagc
     4381 ccagggtatc catcaacgct cctgctcatt gcttccctac tgcttcacga gcgggaggtc
     4441 aatgtagctc tggtggccgg gggcgtgcca tatcttctgc tcagcttttt ggtagtcggc
     4501 ggatttggcc cagaagatat tgtcgacata ggtttgcggg ttgcggtcgt agagggggaa
     4561 ccagctggac tggatctgga ccatgatgcg gtgtccgggc aggaagacat ggtttgcagg
     4621 cggaaggttg aaggtgtagt cgagcggcgt gttgggctgg atcgggtgcg gcacggtgaa
     4681 gctttcgcgg tatcggcctc ggaagatatc ggcggagatc atgagttggt agccgcccat
     4741 gtcgggctga gcggcgactt cgtcggggta aacatcgatg agt
//
LOCUS       DASZYN010000543        12472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1121940, whole genome shotgun sequence.
ACCESSION   DASZYN010000543 DASZYN010000000
VERSION     DASZYN010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 12472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12472
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121940"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1430)
                     /gene="tssK"
                     /locus_tag="VHE33_13880"
     CDS             complement(75..1430)
                     /gene="tssK"
                     /locus_tag="VHE33_13880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HVT98587.1"
                     /translation="MRQLQPVVWSKGVFLSPQHLQAQDRYFEENLRFVSSAVSFRNWG
                     FTSLQLDGAGLSEGRLTLTQAAGIFPDGLLLDMPLSDAPPGSRTLEECFPPGRTTCAF
                     WLAIPQERPSGINVGLQRGTAGTRFYSELQMLRDENSSGVEKPVSLARKNLQILAEGE
                     NLEGSVLLPLAQVQKTEAGSYRLDPEFAPPLLNIKGNELLTGILRSLIETLIARSSQL
                     AGSRRQRNQSLADFSASDIANFWLLYTINSHLPGARHLLEAKQVHPEALFLQMLSLAG
                     ALTTFSPKMEPRDLPQYDHERLGPCFLALDAAITELLETVIPSNFVALPLKPLRDSIY
                     AAAIDKDQYFENSRPYLAIASDLREADLISRAPALLKACSATHIEQLVRQALPGLKLT
                     HVPVPPRAIPVKLRYQYFSVEKSGPVWDAVTRARNFAVYAPGEIPNPEMELIFLFANP
                     E"
     gene            1721..2239
                     /gene="tssB"
                     /locus_tag="VHE33_13885"
     CDS             1721..2239
                     /gene="tssB"
                     /locus_tag="VHE33_13885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HVT98588.1"
                     /translation="MAKESTQHKLDRVRSPRVHITYDVEIGDAIEMKELPFVMGVLGD
                     LTGQPEQPLAALKDRKFVEINPDNFDTVLKGMQPHLAFSVANKLSDDPNAGQLKVDLH
                     FESMDDFSPENVAKQVGPLKELLDLRTRLSDLRGSLQGNDKLDEALFEAVSNTETREK
                     LRAELGKTGGEQ"
     gene            2236..3729
                     /gene="tssC"
                     /locus_tag="VHE33_13890"
     CDS             2236..3729
                     /gene="tssC"
                     /locus_tag="VHE33_13890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HVT98589.1"
                     /translation="MSAQKAQTAAATETVEQTSEQTLLEQIVSQGRFNRDAATMERGR
                     DMVKEFVSQVLAGHMTLTRDAEASIQARIAQIDKLISQQLNEVLHYPAFQKLEATWRG
                     IKYLVDQSETSEMLKIKVLNASKRELLKDLQRAPEFDQSALFRKVYEEEFGVFGGAPF
                     AAMVGDYEFGRGPEDIELLERVSQVASAAHAPFLTAASSDLLNLGSYTQLGAPRDIGK
                     IFDSTEYAKWKSFRASDDSRYVALTLPHILMRLPYGKDTKQIEAFDFEEAVDGTEHSR
                     YLWGNAAYGLAARLTNSFAKYGWCAAIRGVEGGGLVEGLPAHTFRTDEGDVALKCPTE
                     VAITDRREKELADQGLVPLVHCKGTDYAAFFSVQTANKPKLYDKDAANANARVSAQLP
                     YILAMSRFAHYLKAMMRDKIGSFMSRQDCEKFLNQWISNYICADDNASQAAKAKLPLR
                     EAVIQVSEVPGKAGAYRAVAFLKPHFQLDELSVSLRLVADLPASARK"
     gene            3806..4282
                     /locus_tag="VHE33_13895"
     CDS             3806..4282
                     /locus_tag="VHE33_13895"
                     /inference="COORDINATES: protein motif:HMM:NF017454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HVT98590.1"
                     /translation="MAVDYFLKLDGIQGESQESGHKDEVQIMSWSWGATQVSSVAGTG
                     GSGAGKASLSDFTIMANFDKSTPKFFKSICAGTHIKTGTMNAIKSGAEGKPYLKVDFK
                     ELFVSSLQISASSEIPTVSVSFTYNEIKIDYSTQNEQGNLASTGPVTYSTKENKLS"
     gene            4293..5021
                     /locus_tag="VHE33_13900"
     CDS             4293..5021
                     /locus_tag="VHE33_13900"
                     /inference="COORDINATES: protein motif:HMM:NF018699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HVT98591.1"
                     /translation="MDAQSLYRAGQLQPAIEALGGELKKQPLDTRRRTFLFELLCFAG
                     EYDRAAKQLDILADASAEASAGAMLYRSALHAERTRQDMFSRDELPLGSAHAPPPGEL
                     NGASFAELTDADPRIGAHLEVFIAGSYTWVPFAYVESIETAAPRRLRDLLWLPATLHT
                     TADFRLQDLGEVLLPVLSPLSWRNPDDAVRLGRTTIWDQDEKYGEIPLGQKLLRCGED
                     EVPILEMRNLSFHHAGETAASATP"
     gene            5008..6822
                     /gene="tssA"
                     /locus_tag="VHE33_13905"
     CDS             5008..6822
                     /gene="tssA"
                     /locus_tag="VHE33_13905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HVT98592.1"
                     /translation="MPLRDDLLNPIPGDNPSGVSLRYERIYDQIKEARTEEDDSIPAG
                     AWGRTAKKADHQQVIKLAGDALAGKSKDLQLAAWLAEAHVRREGIQLVPASLRLMLDL
                     QEQFWDTLYPEIEEGDSGMRASPLEWAANRIAAILREAPLTRDGLNFYQYRESRAIGY
                     EADAASSDAKREARERAIADEKPTAEDFDKALAATPKTFYVGLEAAMTASGETLDALQ
                     VFCEEKYGDDGPGFGKLRAALEEVGQVVSSLLAEKRKVEPDAVVEEPAAEMPEAEPEP
                     APTEAGSQPAQAASAPAARKTLSAEPADDDDAVRRVQSCAQYLQKNHPASPAAYLLQA
                     SIRLGEMREQGSWASYDFLVSPSTEIRQNLKRLAAESNWEELLRAAVAAVGEPCGRAW
                     LDVHRYVWKAGTEYGYPAVSAAVVSTLQGLLKDIPEIPTWTLNDDTPAANPETLRWLQ
                     ETVLPPPPAVESEPAPVFTTPAETKAAGAEDTPPDVLEIARDFIRRGQLPQAIQLLMR
                     DAAQQPSGRARFQRRLQIAQLCMSAGQGKIAHPVLEELVKEIEERRLEEWEDGEMLSP
                     ALALLLQCLEGADNNGTREALFSRLCRIDPIAAMNISH"
     gene            6842..7303
                     /gene="tssE"
                     /locus_tag="VHE33_13910"
     CDS             6842..7303
                     /gene="tssE"
                     /locus_tag="VHE33_13910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HVT98593.1"
                     /translation="MPRSTETLVTQSVLDRLTAVEEWPATRLQSLRFFKDALKRDLEW
                     LLNTRQPPLPQLESYPLARTSIVNYGLPDITSLGLASASDHRQLRLAIEGCVRAFEPR
                     LTSVRVSIDDADRGDRRLRFHIEGMMKLDPSPEEITFDTVLELASGEYKVK"
     gene            7307..9148
                     /gene="tssF"
                     /locus_tag="VHE33_13915"
     CDS             7307..9148
                     /gene="tssF"
                     /locus_tag="VHE33_13915"
                     /inference="COORDINATES: protein motif:HMM:NF017733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HVT98594.1"
                     /translation="MREELLEYYERELTYLRQLGGEFAQKYPRVASRLLLEQDRCDDP
                     HVERLLEGFAFMAARVHLRVDDDFPEITSALLGIVSPHYLRPIPSMSVVECQLDPDQG
                     KQTAAVQIPAGSALVTRRTVDGLPCRFRTTYPATLWPIATELCEWRQPERLADPVRAQ
                     GAVGVVRLLLRCGRDITFDKLAMRDLAFHLNGETNLVHTLYELLCRNCVSILIRNPQQ
                     RGGKVVALSSAQLRPMGFEPEEAMLPWPKRAFDGYRLLQEYFTFPEKFLFFRLSGLEA
                     IAEAGCTEQAEILFCISRFDRPERQQDLETGVTTRTIRLGCTPVINLQSQVAEPILVT
                     HTKQEYDIIPDVRHRETMELFSIEEVMASNTARRESIRLEPLYSYRFQARRENKRVYW
                     HAIRRMNVFGERQPSSLHLSLVDIDGLLTEPNAEVLTVRCECSNFDLPSRLPFGAEEG
                     DFIAENLPAVRVITALRRPTPTYHPPDARGQLWRLVSQLSLNYLSLDEQGVTGLQEIL
                     RLHNFTDSSYLENQIGGITKMSSSPHFALMSSVYGNLPARGTRVEIELDERQFVGGGA
                     YLFANVLDRFLGSYTSINSFCQLVARTNQRKELLGEWPPKAGYQPLI"
     gene            9112..10158
                     /gene="tssG"
                     /locus_tag="VHE33_13920"
     CDS             9112..10158
                     /gene="tssG"
                     /locus_tag="VHE33_13920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HVT98595.1"
                     /translation="MATQSGLPTADLKFEPIRRLLEQEPCSVQFFQAVRLLERLYPER
                     NPVGLFVAPSSEVVRFSSLPTLSFPASEIAELDLADESSSAHMKVNFMGLSAAVAALP
                     DPYTEFLLERAKAKDPAPGEFFDLFNHRMISLFHRGWQKYRFYIAYERTGASDDGISR
                     ILLHLIGLGSKGLTRRMEIEDEACLYYTSLIAQRRPTAQGLKQLLEDYFEVPVRIEQF
                     TGTWNRLPPANQTFLRDNGSFCERLGMGTIVGDEIWDQHGTITLRLGPMNFATYLEFL
                     PGGPAFIELRAWLHFYANREFNFVIQPVLEREETPPTQLGDEGETASRLGLVSWVKNQ
                     PLHRDPDEATYRLS"
     gene            10188..>12472
                     /gene="tssH"
                     /locus_tag="VHE33_13925"
     CDS             10188..>12472
                     /gene="tssH"
                     /locus_tag="VHE33_13925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HVT98596.1"
                     /translation="MSLNLKSLIGKLNDSTRNALEAAAGLCVARTHYDIEIEHFLLKV
                     LDNADNDIAFILKQYGVDRSRLTAELQRSLDRLKSGNARSPAFSPHLVKLLTEAWTVA
                     TIEYDAAQIRTGFVLLALLQSDDLSRMIREVSKELQLVPVEAFRKDFYTVTEKSHEAT
                     ESSPAAAPGDSGEAPRSSGSGKTQHLDQYTVNLTAHAKAGKIDPVLGRDAEIRQVIDI
                     LMRRRQNNPILTGEAGVGKTAVVEGFALRLAAGDVPPALRNVQLHSLDMALLQAGAGV
                     KGEFENRLKGLINEVKSSPHPIVLFIDEAHTMIGAGGQAGQNDAANLLKPALARGELR
                     TIAATTWSEYKKFFEKDAALARRFQVVKVEEPSETQCDAMLRGMVSALEKHHQLRILD
                     EAVTSTVRLSHRYLAGRQLPDKAVSVLDTACARLALGQNTTPPAIEDATRRIDDLRVQ
                     KQILERETTVGEDHAERLAAIDEQLAASEATVARLKTQWEQEVELVRQIRAMRNALET
                     GAETIPAEGDGKTGVKPSLQALEALEEQLKTLQGETPLLRVCVDEQIVGEVISAWTGI
                     PVGKMVKDEIASVLDLDKHLRSRVIGQDHALGAIAQRIITSRARMDDPGKPVGVFMLV
                     GPSGVGKTETALALSDLLYGGERNLITINMSEFQEAHTVSTLKGSPPGYVGYGEGGVL
                     TEAVRRRPYSVVLLDEVEKAHPDVLELFFQVFDKGNMEDGEGRSIDFKNTIIILTSNA
                     ATDTLMKLTADKETMPDSEGLA"
BASE COUNT         2583 a         3859 c         3690 g         2340 t
ORIGIN      
        1 tggtgaatgt gttcttgctg gtggcgatcg tgctgattct ggtcttcgtc ctgcgtcacc
       61 actgacggac gcgcttactc gggattcgcg aacaggaaaa tcaactccat ttccggattc
      121 ggaatttcgc ccggggcgta aaccgcgaag ttgcgcgcgc gtgtcactgc gtcccacacc
      181 ggcccactct tttcgacgct gaagtactgg tagcgcagct tcaccgggat cgcacgtgga
      241 ggcaccggca cgtgcgtgag tttgaggccg ggaagcgcct ggcgaaccag ttgttcaatg
      301 tgcgtggccg agcatgcctt cagcagcgcg ggcgcgcggc tgatgagatc ggcttcacgc
      361 agatccgagg cgatggccag ataaggcctc gaattctcga agtattggtc tttgtcgatc
      421 gctgcggcgt aaatcgaatc gcgcagcggc ttgagcggca gtgcaacgaa gttgctggga
      481 atgaccgtct ccagcaattc tgtgatcgcc gcgtccagcg ccagaaagca tgggcccaga
      541 cgctcgtggt cgtactgcgg cagatcccgc ggctccatct ttggagagaa ggtggtcagc
      601 gctcccgcca gagacagcat ctgcaggaag agcgcttccg gatgcacctg cttcgcttcc
      661 agcaaatggc gcgcccctgg caggtgcgag ttgattgtgt agagcagcca gaaattcgca
      721 atgtccgatg cgctgaagtc ggccagcgac tggttgcgct ggcgccgaga accggcaagc
      781 tgactgctgc gcgcaatcag cgtttcgatc aggctgcgca ggatcccggt taaaagctca
      841 ttgcctttga tgttgagcag cggcggagca aactccggat cgagccgata gctgcccgcc
      901 tccgtcttct gcacctgagc gagcggaagc agaaccgatc cttcgaggtt ctctccttct
      961 gccagaatct ggaggttctt gcgcgccaga gagaccggct tctcgacgcc cgagctgttc
     1021 tcgtcccgca gcatctgcaa ctcggaatag aatcgcgttc ctgcagtacc ccgctgcaac
     1081 ccgacgttga tgccgctggg gcgctcctgc ggaatcgcca gccagaaggc gcacgtcgtg
     1141 cgccccggag gaaaacactc ttccagagtg cgcgatcccg gaggcgcatc ggacagtggc
     1201 atatccagca gcaaaccgtc ggggaagatt cctgctgcct gggtcagagt caggcggcct
     1261 tcgctcagac ccgcgccatc cagctgaagc gatgtgaatc cccagttacg aaaggagacg
     1321 gcgctcgaca caaagcgcag attctcctcg aagtagcggt cctgcgcctg cagatgctgc
     1381 ggcgacaaaa acactccttt ggaccacacc acaggctgca gctggcgcat gcgtcgactc
     1441 cgtgagggat tgagtatact gcaacgcgac tctctccccg gaggcctgaa acggacggcg
     1501 cgctgccttc actatcgtgg gaaaggcttg acacggaccc aggtacacag gcaataatca
     1561 gtccgcccct gcaaccgaac gcaaggacct cccagtccga cgtctgcgca tccgttcgac
     1621 gacagcagaa gagtacgatc tcaggaccgt tgttccgggg aggcagagtt cttagcgaca
     1681 gttgagctgt gccttatgcg ccggctcgga ggatggattc atggccaaag aaagcactca
     1741 gcataagctg gatcgggtac gctcgccgcg cgtgcacatc acatacgacg ttgaaatcgg
     1801 cgacgccatc gagatgaagg aactgccctt tgtgatgggg gttctgggcg atctgaccgg
     1861 tcagccggag cagccgctgg cagcgctgaa ggaccgcaaa tttgtggaga tcaatcctga
     1921 caacttcgac accgtcctca aagggatgca gccgcacctc gccttttccg tcgccaacaa
     1981 actgagcgac gatcccaatg ccgggcagct gaaggtcgac ctgcactttg agagcatgga
     2041 tgacttctcg ccggagaatg tggcgaagca ggtcggcccg ctcaaggagc ttctcgattt
     2101 gcgcacccgg ctgtcggatc tgcgcggaag cctccagggc aacgacaaac tcgacgaggc
     2161 cctcttcgag gctgtgtcaa ataccgaaac gcgcgagaag cttcgcgccg agctgggcaa
     2221 gaccggaggt gagcaatgag cgctcagaaa gcgcagaccg cggccgcgac cgagaccgtc
     2281 gaacaaactt cagaacagac gctgttggag cagattgtct cccaggggcg gttcaatcgc
     2341 gatgcggcga ccatggaacg cggccgggac atggtgaagg agttcgtctc ccaggtcctt
     2401 gcaggccaca tgacgctcac gcgggatgcc gaagcgtcga tccaggcgcg cattgcgcag
     2461 atcgacaagc tcatctccca gcagctgaac gaagtcctgc actatccggc atttcagaag
     2521 ctggaagcga cctggcgcgg catcaagtat ctcgtcgatc agtccgaaac cagcgagatg
     2581 ttgaagatca aggtgctgaa cgcctccaag cgcgagctgc tgaaagacct gcagcgcgca
     2641 ccggagttcg atcagagcgc gctgttccgc aaagtctatg aagaggaatt cggcgtcttc
     2701 ggtggcgcgc cgttcgcggc gatggtgggc gactacgaat tcggtcgcgg gccggaagac
     2761 atcgaattgc tggagcgagt ttctcaggtt gcctccgcgg cccatgcccc attcctgacc
     2821 gccgccagct ccgatctgct taatctgggc agctacacgc agttgggcgc gccgcgcgat
     2881 atcggtaaga tctttgattc gaccgaatac gcaaagtgga agtcgttccg agcgtcggac
     2941 gattcacgtt atgtggccct cacgctgcct cacattctca tgcggctccc ttacggcaag
     3001 gacacgaagc aaatcgaagc cttcgatttc gaagaagccg tcgacggcac cgagcactcg
     3061 cgctatctgt ggggcaatgc cgcctatggt ctcgcggcac ggttgacgaa ttccttcgcg
     3121 aaatacggat ggtgcgccgc aattcgcggc gttgaaggcg gcggattagt cgaaggcctg
     3181 cccgcgcata ccttccgtac cgatgaaggc gatgtggcgc tcaagtgccc taccgaagtc
     3241 gccatcaccg atcgccgcga aaaggaactc gcagatcagg ggctggtgcc gcttgtccat
     3301 tgcaaaggca ccgactatgc ggccttcttc agcgtgcaga cggccaacaa gccaaagctc
     3361 tacgacaagg acgctgcgaa tgccaacgcc cgcgtgtcgg cgcagcttcc ctacatcctg
     3421 gccatgtccc gcttcgcgca ttacttaaag gcgatgatgc gcgacaagat cggcagcttc
     3481 atgtcccgcc aggactgcga gaagttcctc aatcagtgga tcagcaacta catctgtgcg
     3541 gatgacaatg cttcgcaagc cgccaaggcg aaactgccgt tgcgggaagc cgtcattcag
     3601 gtttccgaag ttcccggcaa ggctggagct tatcgcgccg ttgcctttct caaaccgcat
     3661 ttccagctcg atgagctctc tgtctcgctg cgactggtgg cggatttgcc cgcttctgca
     3721 cgcaagtaaa gcctgttcac cttcgctgta ttcagaccga accggcaggc gcccaagcgc
     3781 gcctgcgtca tgaaaggagt ttgcgatggc agttgattac tttttgaagc tggacggaat
     3841 ccagggcgag tcccaggagt ccggacataa agatgaagtt cagatcatga gctggagctg
     3901 gggcgccacg caggtgtcgt cagtcgcggg cacgggagga tcgggtgccg gtaaggccag
     3961 cctgtccgat ttcaccatca tggcaaactt cgacaaatcc acaccgaagt tcttcaagtc
     4021 gatctgcgcc ggtacccaca tcaagactgg caccatgaat gcgattaaat cgggcgcgga
     4081 gggaaagcct tacctgaagg tcgatttcaa ggagctgttc gtcagcagcc ttcagatctc
     4141 ggcctccagc gagattccca ccgtcagcgt ttcttttacc tataacgaga tcaaaatcga
     4201 ctactcgacc cagaacgagc agggcaatct cgccagcacc ggccctgtga cctacagcac
     4261 gaaggaaaac aaacttagct aaggcgatcc gtatggacgc gcagtccctg taccgcgccg
     4321 gtcagctgca accggctatc gaagcgcttg gtggcgagct gaagaagcag ccgctcgaca
     4381 ccaggcgccg tacttttctt ttcgaattgc tgtgctttgc cggcgagtac gaccgggcgg
     4441 ccaaacagct ggatatcctc gctgatgcca gtgccgaagc ttcagccggc gcgatgctct
     4501 atcgctccgc tctgcacgcc gaacgcacgc gccaggacat gttttcccgc gacgagcttc
     4561 cgctcggttc ggcgcatgcg ccgcctccag gcgagttgaa tggcgcctcc tttgccgaac
     4621 tcaccgatgc cgatccacgc atcggcgcgc atctggaggt cttcattgcc ggcagctata
     4681 cctgggtacc cttcgcctac gtcgagtcga ttgaaacggc cgcgcccagg cggctgcgcg
     4741 atctgctgtg gctgcccgca accctgcata ccaccgcgga ctttcgactg caggatctcg
     4801 gcgaagttct gctgccagta ctgagccctc tctcctggag aaatcctgat gatgccgtgc
     4861 gtctggggcg aaccaccatc tgggatcagg atgagaagta cggcgagatt cccctcggac
     4921 aaaagctgct gcgctgcggt gaagacgaag tgcccattct ggagatgcgt aatctgagtt
     4981 tccatcacgc gggggagaca gcggcaagtg ccactccgtg acgatctgct gaatccgatc
     5041 ccgggagaca acccgtcagg cgtgagtctg cgctacgaac ggatttacga ccagattaag
     5101 gaagcacgca ccgaggagga tgattccatc cccgccggtg cgtggggccg cactgccaaa
     5161 aaggccgatc atcaacaggt gatcaagctg gcaggcgacg ctctggcagg caagagcaag
     5221 gatctgcaac tggcagcatg gctggccgaa gctcacgttc gccgcgaagg catccagctg
     5281 gttcctgcat cgctccggct catgctcgat ctgcaggagc agttctggga cacgctttat
     5341 ccggagattg aagagggcga ttccggcatg cgcgcctctc ccctggaatg ggctgcgaat
     5401 cggatcgcgg caatcctgcg ggaagcgccc ctgacgcgcg atggcctgaa cttctatcag
     5461 taccgcgagt caagagccat cggctacgag gccgatgctg cttccagcga tgcgaagcgg
     5521 gaagcgcgcg agcgtgcgat cgcggacgaa aaacccaccg ccgaggattt tgacaaggcg
     5581 ttggccgcca cccccaagac gttttacgtc gggttagaag ccgcgatgac cgcttccggc
     5641 gagacgctcg atgcactgca ggttttctgc gaagagaaat acggtgacga cggcccgggg
     5701 tttggaaagc tgcgcgccgc gctcgaagaa gtaggccagg tcgtctcgag tttgttggcg
     5761 gaaaagcgca aagtggaacc cgacgcggtc gtggaagagc cggccgcaga gatgccggag
     5821 gcagaaccgg agccggctcc caccgaagca ggctcgcagc cagcgcaggc ggcatcggct
     5881 ccggccgcca ggaaaactct ctccgcggag cccgctgatg acgacgacgc agttcgtcgc
     5941 gtgcaatcgt gtgcgcaata tctgcagaaa aaccatcccg ccagtccggc ggcctatctc
     6001 ctgcaggcat cgattcgact cggcgagatg cgcgaacagg ggagctgggc gtcgtacgat
     6061 ttcctggtct ctccgtcaac cgaaatccgg cagaacctga agcgcctggc tgcggagtcg
     6121 aactgggagg aattgttgcg cgccgcggtc gccgccgtgg gtgagccttg cgggcgcgcg
     6181 tggctggacg ttcaccgcta cgtgtggaag gccggcactg aatacggcta tcccgccgtt
     6241 tcggctgcgg tcgtttccac tttacagggg ctgctgaaag acattcccga aataccgacc
     6301 tggacgctga acgacgacac gcccgcggcc aatcccgaaa ccctgcgctg gctgcaggag
     6361 accgtactgc ccccgccgcc tgcggtggaa tccgagccgg cgccggtatt cacaactcca
     6421 gcggagacaa aggctgcagg cgcagaggat acccccccgg atgtgctgga gattgcgcgc
     6481 gacttcatcc gccgagggca actgccccag gccatccagt tgttgatgcg cgatgcggcg
     6541 cagcaaccca gcggacgcgc ccgcttccag cgccgcctcc agatcgcgca gctgtgcatg
     6601 agcgccggac agggaaagat cgcgcatccg gttctcgaag aactggtgaa agaaattgaa
     6661 gaacgtcggc tggaggaatg ggaagacggc gagatgcttt cgccggcact ggcattgctg
     6721 ctgcaatgcc ttgagggcgc cgacaataac gggacaagag aagcgctctt ctcccgtctg
     6781 tgccgcatcg atcccatcgc agccatgaac atctctcact gacgcagagg actcgcagcg
     6841 catgccacgt tcgacagaga cactggtcac acaatccgtg ctcgatcgcc tcaccgccgt
     6901 ggaagaatgg ccggctaccc gcctgcagtc gctgcgcttc tttaaggatg ccctcaagcg
     6961 cgatctggaa tggttgctca atacgcgcca gcccccgctg ccacagttgg agtcttatcc
     7021 gctggcacgg acatcgattg tgaattacgg actgcccgac atcaccagcc tcggcctggc
     7081 ttcggcttcc gatcatcgcc agttgaggct cgccatcgaa ggctgcgtgc gcgcctttga
     7141 gccccgcctg accagcgtgc gtgtttcgat cgacgacgcc gatcgaggag accggcggct
     7201 gcgttttcat attgaaggca tgatgaaact cgatccgtct ccggaagaaa tcacctttga
     7261 cacggtactg gaactagcca gcggtgagta caaggtgaag tagaccatgc gcgaagaact
     7321 tctcgaatac tacgaacgcg agctgaccta cctgcgccag ctgggcggag agttcgccca
     7381 aaaatatccg cgcgtggcca gccgccttct cctcgaacag gaccgttgcg atgatccgca
     7441 tgtcgagcgg ctgctggagg gattcgcctt catggcggcg cgcgtccatc tccgcgtcga
     7501 tgacgacttc cctgagatta catccgccct gctgggcatc gtttcgccgc actacctgcg
     7561 gcccatccct tccatgtccg tcgttgaatg ccagctggat cctgaccagg gcaagcaaac
     7621 tgctgcggtg cagattcccg ccgggagtgc tctggtgacc aggcgtaccg tcgatggatt
     7681 gccgtgccgg tttcgaacta cctacccggc aaccctgtgg ccgatcgcga cggaactgtg
     7741 cgagtggcgg caacccgaac gcctggcgga tccggtgcgc gcgcaggggg cggttggagt
     7801 cgtgcgcctt ttgctgcgct gcggacgcga catcaccttc gacaaattgg cgatgcggga
     7861 tctcgccttc cacctgaacg gggagacgaa tctcgtccac actctttacg agttgctgtg
     7921 cagaaactgc gtttccatcc tgatccgcaa tccacagcaa cgagggggga aggtcgtcgc
     7981 cctctcctct gcgcagttgc ggccgatggg atttgagccc gaagaagcga tgctgccctg
     8041 gcccaaacgc gccttcgacg gctaccgtct gctgcaggaa tacttcacgt tcccggaaaa
     8101 gtttctcttc ttcaggctga gcggtctgga agcgatcgcc gaggctggat gcacggagca
     8161 ggccgagatc cttttctgta tctctcgttt cgatcgcccc gaacgccagc aggacctcga
     8221 aacgggcgtg accacgcgaa ccattcgcct cggctgcacc ccggtgatca acctgcagtc
     8281 gcaggtcgcc gaaccgattc tcgtcaccca tacgaagcag gaatacgaca ttatccccga
     8341 cgtgcgccat cgcgaaacca tggagctgtt ctcgatcgag gaagtcatgg cctcgaacac
     8401 ggcccggcgc gagagcatcc gcctggagcc gctctattcc tatcgctttc aggcccgcag
     8461 ggagaacaag cgcgtttact ggcatgccat ccggcggatg aacgtctttg gcgaacgcca
     8521 gccctcttcc ctgcacctct cgctggtcga catcgacggc ctgctgaccg agccgaatgc
     8581 cgaggtgctc actgttcgct gcgagtgttc gaattttgac ctcccgtccc gcctgccttt
     8641 cggagccgaa gagggggatt tcatcgctga gaatttgccg gccgtgcgcg tgatcacggc
     8701 cctgcgccgc cccaccccga catatcaccc cccggatgcc aggggtcagc tgtggcgcct
     8761 ggtttcccag ttgtcgctca attacctgtc cctcgacgag cagggggtga ccggcctcca
     8821 ggagattctg cgtctgcaca acttcaccga ttcctcctat ctcgaaaatc agattggcgg
     8881 aatcacgaag atgagttcca gcccgcattt tgcgctcatg agttccgtct acggcaatct
     8941 gccggcacgc ggcacccgcg tcgagatcga gctggatgag cgtcagtttg tcggcggcgg
     9001 cgcttatctt tttgccaacg tgctggatcg ctttttggga agttacacct ctatcaacag
     9061 cttctgtcaa ctggttgcgc gcaccaatca aagaaaggaa ttgcttggcg aatggccacc
     9121 caaagcgggc taccaaccgc tgatctgaaa ttcgagccga tccggaggct gctcgaacag
     9181 gagccctgct cggtccagtt cttccaggcg gtgcgcctgc tcgagcggct gtacccggaa
     9241 cgcaatcccg ttggcctgtt cgttgctcca tcatccgagg tggtacgttt ctcttccctt
     9301 ccaaccctgt ctttcccggc cagtgaaatt gccgagcttg acctcgcgga cgaatccagc
     9361 tccgcgcaca tgaaggtgaa cttcatgggc ctcagtgccg cggtcgctgc tttgcccgat
     9421 ccctataccg agttcctcct cgagcgcgct aaagcaaaag acccggcccc cggcgagttc
     9481 ttcgatctgt tcaaccaccg catgatttcc ctcttccatc gcggttggca gaaataccgg
     9541 ttctatatcg cgtacgagcg gactggagcc agtgacgacg gcatcagcag aatcctgctc
     9601 catctgatcg gcctgggcag caaaggcttg acgcgccgta tggaaatcga ggacgaggca
     9661 tgcctctact acacgagcct catcgcccag cgacgcccca ccgcccaggg gttaaagcag
     9721 ctgcttgagg actacttcga agtgcccgtc cggattgagc agtttacagg gacgtggaat
     9781 cggctgcccc cggccaatca gaccttcctg cgggacaacg gatccttctg cgaacgcctg
     9841 ggaatgggaa ccatcgtggg cgacgaaatc tgggatcagc acgggaccat cacgctgcgg
     9901 ctgggcccga tgaatttcgc cacatacctg gagttcctgc ccggcggccc ggcctttatc
     9961 gaattgcgcg cgtggctcca cttctatgcc aatcgcgaat tcaatttcgt catccagccc
    10021 gtgctcgagc gcgaagaaac gccgcccacg cagctgggcg acgagggaga aacagcgtca
    10081 cgtctgggtt tggtgagctg ggtcaaaaac cagcctctcc atcgcgatcc cgacgaggct
    10141 acctaccggt tgtcgtaaca gattgtgcat cactggagca agggaccatg agcctgaatc
    10201 tgaaatcgct gatcggcaag ctgaacgaca gcacacgcaa tgcactggaa gccgcggctg
    10261 gactgtgcgt tgcccggacc cactacgaca ttgagatcga gcatttcctg ctcaaagttc
    10321 tcgacaatgc cgataacgac atcgctttca tcctgaagca gtatggggtc gatcgctcaa
    10381 ggctcacagc cgagctccag cgcagcctcg atcgcctgaa aagcggcaat gcgcgcagcc
    10441 ccgcctttag cccccatctg gtgaagctgc tcaccgaggc gtggacggtg gcgaccattg
    10501 aatacgacgc cgcccagatt cgcactggct ttgttctgct ggctctcctg cagagcgacg
    10561 acctgtcgcg aatgattcgc gaggtaagca aagaactgca gctggtcccg gtggaagcct
    10621 ttcgtaagga tttttacacc gtcaccgaaa aatctcacga agcgacagag tcctctcccg
    10681 ccgccgcacc cggtgacagt ggagaagcgc cccggtcgtc aggttcaggg aagacgcaac
    10741 atctcgatca gtacacggtg aacctgaccg cgcatgcgaa ggcaggcaaa attgatccgg
    10801 tcctcggccg cgatgcggag atccgccagg tgatcgacat ccttatgcgc cgccgccaga
    10861 acaaccccat cctcactggc gaagccggcg tcggtaagac cgccgtagtc gagggctttg
    10921 cgctgcgatt ggctgccggc gacgtgccgc ccgctctgcg caacgtgcag ctacacagtc
    10981 tggacatggc gctcttgcag gccggagccg gagtgaaggg ggaattcgag aaccggctga
    11041 aagggctcat caatgaggtg aaatcctcgc cgcatcccat cgtgcttttt atcgatgagg
    11101 cccacaccat gatcggcgcg ggcggccagg ccggtcagaa cgatgcggca aatctcctca
    11161 aacccgcgct ggcacgcggc gaactgcgca ccatcgcggc gacaacctgg tccgaataca
    11221 agaaattctt tgagaaggac gcggcgctgg cccggcgctt ccaggtagta aaagtggaag
    11281 agccctccga aacccagtgc gacgcgatgc tgcgcggaat ggtctccgcg ctggagaagc
    11341 accatcagct gcgaattctc gatgaggcgg tgacctcaac agtgcggttg tcgcaccgct
    11401 acctggctgg acgtcagctg ccggacaagg ccgtgagcgt actcgatacc gcatgcgctc
    11461 gcctggctct cggccagaac accacgccgc ccgctattga agacgcaacc cgccgcatcg
    11521 acgatctgag ggtgcaaaag cagatcctcg agcgggagac caccgtcggc gaagatcatg
    11581 cagagcgcct tgcggcgatc gacgaacaac tggcggccag cgaagcaacc gttgccaggc
    11641 tcaaaacgca atgggaacag gaagtcgagc tggtgcgtca gattcgtgcc atgcgcaacg
    11701 ccctcgaaac cggcgccgaa accattcctg cggagggcga cgggaaaacc ggtgtgaagc
    11761 cttcgttgca ggcactcgaa gctcttgaag aacagctgaa gaccctgcag ggcgagactc
    11821 ccctcctgcg tgtctgtgtc gacgagcaaa tcgtcggcga agtcatctca gcctggaccg
    11881 gcattcccgt cggcaaaatg gtgaaggatg agatcgcttc agtgctcgat ctggacaagc
    11941 acctgcgcag ccgcgtcatc gggcaggatc acgccctcgg cgccatcgcg cagcgcatca
    12001 ttacctcccg cgcgcgcatg gacgatcccg gcaagcccgt tggagtcttc atgctcgtcg
    12061 gtcccagcgg cgtcggcaag accgaaaccg ccctcgcgct gtccgatctc ctctacggcg
    12121 gcgaacgcaa tctcattacc atcaacatgt cggagttcca ggaagcgcat acggtttcta
    12181 ccctgaaggg ttcgcctccc ggctacgtcg gctacggcga aggcggagtg ctcaccgagg
    12241 ccgtccgccg tcgcccctat tcggtcgttc tcctcgatga ggtagagaag gcgcatcccg
    12301 atgtgctcga actcttcttc caggtcttcg ataaaggcaa tatggaagac ggcgaaggcc
    12361 gatccatcga cttcaaaaac accatcatca tcctcaccag taacgccgcc accgacaccc
    12421 tgatgaagct gaccgccgat aaagagacca tgcccgacag cgagggtctt gc
//
LOCUS       DASZYN010000544        32912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_730608, whole genome shotgun sequence.
ACCESSION   DASZYN010000544 DASZYN010000000
VERSION     DASZYN010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 32912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32912
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730608"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VHE33_13930"
     CDS             <1..1047
                     /locus_tag="VHE33_13930"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HVT98597.1"
                     /translation="PGTRFYNVYPLFHTASVYEGFRRDFGDSRRVMSLARAAYLGAQR
                     NGTIFWSSDIVSTWDMLKRSIPAGLNFTASGMPYWDTDIAGFFSPQLSSDYHAEHKPL
                     IDGSDVRDNIGNYEDYPELFVRWFEWGAFQPVMRAHGERMHNEVWAYGKQAEPILAKY
                     LKLRYQLLPYTYSAAWKSYQTGAPYTRALFMDFPNDPKVTDIPDEYMYGPALLVAPVS
                     QQGATTRSVYLPAGTDWYNFWTKEKYHGGQTITANAPIDTLPLFVRAGSIIPIGGAVE
                     SAAQKQTIAKVEVWPGADADFALFQDDGETYNYEKSGGDVTKLHWDDRSQRLTHTGHP
                     AWTQPDSELLEVIH"
     gene            1153..3162
                     /locus_tag="VHE33_13935"
     CDS             1153..3162
                     /locus_tag="VHE33_13935"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HVT98598.1"
                     /translation="MNDLTRLRKARYGKAVVWLFALGFGCSSPAQTRVVPSRLAPGQS
                     LPAESSEYRQVQQRLAQGWNTWDVHSVTTQVLLPQGLAIHTSLKHNTTEGSDAFLGDT
                     LIGRLTPHAEQVTPGPHAWDGSYTDLRVAWHGHEWRMQSAQADGELVMLATPLSGAQG
                     SALPPTIIFSVDFLWNRPGTITGHDDFIEAQSPTGTARVFCTCAPAGSKMRDQWIDSP
                     VRGPFFSADFTGPIGIGTGKRRTLDEIRVAVEKQRQAYEESIHAAGSNAPIVDAIETT
                     LGWDTIYEPAGERVVSPVSRVWSVDWGGYVIFDWDNFFAATMAGIGDRDLAYANALET
                     LRGETNQGFVPNYARGGGWKSFDRSEPPVGAVTVLGLYRKSHDRWFLRDAFDPLLRWN
                     RWWATHRDIDGYLAWGSDGDNSPGNLDDQSRGTRVGAVLESGLDNSPMYDTATYNEQK
                     HVLEYADVGLMSLYIADCDALAKIAQELGMAEPEKELTERAARYRAKLATLWNDEKGI
                     FLNKDLHTGQSNTRLSPTNFYPMLAHAATPDQAHTMIAKHLKNPAEFWGQWVIPSIER
                     DDPAFHDQNYWRGRIWGPMNYLVYLGLCNYDDAAVRQEFAQKSYALFLKEWTEHRHVH
                     ENYNSILGSGDDVSNSDRFYHWGALLGYIEYLEASGAGKRPPSGD"
     gene            3448..4968
                     /gene="gltX"
                     /locus_tag="VHE33_13940"
     CDS             3448..4968
                     /gene="gltX"
                     /locus_tag="VHE33_13940"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HVT98599.1"
                     /translation="MPDPTPTSPRVRFAPSPTGYLHVGGARTALFNWLFARHFGGTFI
                     LRIEDTDFERSSEAMVEGILEGMRWIGLDWDEGPFFQSQRLPLYQATAQKLIDSGHAY
                     YCFCTKEELEARRAQATAAGGRAMYDRRCRSISPVEAKKRCLDGHSAPAVRFAVPDGV
                     TSWNDAVFGKVEFANAELEDFVLLRSSGAPNDSPSSLGCLDGIPTYHLSVVADDIDMR
                     LTHIIRGADHISNTPKQILQYHALGAALPTFAHVPLILGPDKTRLSKRHGATSVIAYK
                     DMGIVPEAFLNFLALLGWSPRSAQSSKQNPQQQDREVFTSDNLIQLFTLEGISKSNAV
                     FDNDKLAWFNTEYIRSSDPDKLLPLIQNEWKAAGFVPTRTPAEVLAAIEFIRPRARSL
                     KDFATLFRAYFSDDFEYDPAAVTKFLADPALPGLLQDLAHRYAAAPDLTEASAEEILR
                     ALAAEKGLKAGALINGARVALTGQAVAPSLFAVMALLGKDRVVRRLSAVDRVPTSG"
     gene            5198..5274
                     /locus_tag="VHE33_13945"
     tRNA            5198..5274
                     /locus_tag="VHE33_13945"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5232..5234,aa:Asp,seq:gtc)
     gene            5642..7540
                     /locus_tag="VHE33_13950"
     CDS             5642..7540
                     /locus_tag="VHE33_13950"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HVT98600.1"
                     /translation="MPGIGGPLRGETLGAMTDDGVQSGRSLKELLAIEEALRVSEQRY
                     RVAFETSLDAIGITRIDDGMFVDVNRQFFTMVGYTREELVGQTSLQGCTWTDSEGESH
                     PAEFIDVTGRSTLDIGLWEDSAQRAELMAILRREAVCRGFEARFRRKNGELFWVQLSA
                     SVIQLDGVDCVLFVTRDITAAKAAEEEIRNLSLYDDVTCLPNRRHLLERLQARQQTAM
                     GNAKSALLSISLDHFRTFNDSFGGAAGELLLREMARRVETCVRECDTVARSGGAEFAV
                     LLGHLCGSREDAAGQAQEIARRVLERISEPCGVAGQICHCTASIGITIFQERGLSGEQ
                     IVQQSDIAVDHARVAGRNTIRFFAPALQTAISERVAMEADLRRAMTSNELMLCYQPQM
                     DRGNLTGAEALVRWRHPTRGLLLPSAFIPVAEEAGLIVPLGEWVLENACRQLARWSHS
                     GKMRDLTLAVNISAWQFRQPDFTEKVLDTIRRHQVDPQRLELELTESSLVEDVDAVVM
                     RMGELKAHGLRFSVDDFGVGYSSLSYLQRLPLDKLKIDMQFVRNLLIDPGSRAIARAI
                     ISLSQALGLRVIAEGVESEQQRKHLSELGCDAWQGFLCSQPVVAEEFEELVAGEKAQR
                     LPARTGSR"
     gene            complement(7553..9421)
                     /locus_tag="VHE33_13955"
     CDS             complement(7553..9421)
                     /locus_tag="VHE33_13955"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HVT98601.1"
                     /translation="MSHSRKVLAAVLCTMAFAATCFAQPNNSFSVITGPNNIFVSSTD
                     SSSPTPRKIYAVDLNNDGVPDLVQDEVSYDATYGNGQFAVSIANGDGTFKAPVAVNYP
                     PTVAFAPMAFGDFNNDGHLDIAMPVAGTTVLAIYLGKGDGTFVNPWYFNLTLASGQTL
                     DNNAIVSADFGRHGEADLALTASDSSNSTLYVLPGGGNGVFQAAHPVLTAPTQTGFGD
                     PIISMSVGDFDGDDNADIVASVITYNGSTGNIDNTAVHVLYGEGNFDLIDTTPYSVPG
                     GEFQVGAADLDSDGFSDFYVFDTDAWVLKTFYGEWGRGFDQHSTAIPSAYYAGAPAVA
                     DFNDDGHMDLLFTSTSYMVFLRGEADRGAFTTQTWSLPPGGGPPQNPNGYITAPVAGD
                     FNHDTRPDFVVARYVAPSAGTSTVDTGINTSTVGLWSNCSYPTSARGIMNCSPVASSG
                     TTVNFNATAHSLNKLRKIELWVDGNKLGEEYHTWGDNGGSNAWFNFSSTLAAGSHQGT
                     YFAVDIDNRLQQSNFTFNIPSTCAAPTSAGVHLCSPSTNSNQPAGPVNVGATATVTGT
                     LARMEIWVDSTKQYTETNSTTLWAAVQVPHGQHTITVFAVNTGGTVWSQAATVTLP"
     gene            9756..12755
                     /locus_tag="VHE33_13960"
     CDS             9756..12755
                     /locus_tag="VHE33_13960"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HVT98602.1"
                     /translation="MHRFVLSRWCGSLLFVLLTAVTSLAQQLATGSIRGTVIDASGRP
                     VAHAQVTLKNDRQGTERSFSTQNDGEFTFETLEAANYTLTATGTGGFAEWREPLTLDV
                     GQMLSIPVHLVVAGAPTTVQVSADRQRVDTTDSVVGGVIESRQIETLPLNGRNYLELS
                     YLVPGNAPAPNFDPTKGNTVVVSSAGQTGRGGNVTIDGADNNDDVVGGSLVNIPEDAV
                     QEFQIATNRFSAALGRSGSTVVNVVTKQGSNALHGGIGFYERDRVLQALPATYDPTIG
                     STPPFHRQQYAGEVGGPIRHDKAWWFVAMEDRQQQGADLVGVRDSSSRTIHRQFATAP
                     LHDYLTTERVDFQMTPRDRVGVRESLELEDDLSQSTLDRALATAAYRQTAVNHLQGLA
                     ADWVHIFTPAVINRMSFADNNFMNTTAPTSTAPQITYPDLDDGATYRVPQKTKQFRLQ
                     GDDVLSWSRHNHALSLGGEVQWIDADFYLGVFQQGNIQTVEDFPDFDRNQDGKVDDDD
                     LLFAVALRSSTPQRALVLPNNDNYHLAGFFEDDWRVHPRLTLNAGLRWEMDTNLNNLS
                     WYENRNPIVQSFYSGTRHRDFNDWGPRFGFNYAFRPNLSLHGGYGIYYDRITLEVMSL
                     EKGFDGRALALNVTAGNVITDPGGNPVYLNPDGTFAPGAPQVLSNPFGGFIFTGAGAA
                     GIDVINNRMRNPMVQQFNLGIEKEFSRGLFVQVDGVHNLGTRFIIGRPVGSVFNSETQ
                     GPDGVTDLESAVNTKYDGLWVSVRQHLARYGEFDAAYTLAKAYNYANDDQIPFEYSPV
                     DPNDLHREYGPPPNDQRQRLVLSGVGNLPWKLRFAPIWTIASGVPMDILLPDASERVP
                     TLQRNAGGREFHNAQQLNAWISKTNAAGGVYEASANGYVMLPQVSNKARFNDSFNAMD
                     FRLDRTVSFGDRYSLDLIGEAFNIFNVTNILGVSNLNYSGYANTLSPDSGNPSFSSSF
                     GMPASTAGGVFGSGGPRAFQFAAKLAF"
     gene            12784..13404
                     /locus_tag="VHE33_13965"
     CDS             12784..13404
                     /locus_tag="VHE33_13965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01926.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HVT98603.1"
                     /translation="MSRPEYELLARLRPSPVEQSETKIAMVAESEAEGETAAVYQYFR
                     DQTGRQDVPGILKCFATDPGYARHMVDLSSSLLFSDGKLSRRHKEMIATWVSSLNRCP
                     YCFDSHGFFLQVQGGSDALLRSFAERDLNGAALTDAEQKLLAYVEKVNGASFRTSRED
                     VESLIAAGWSEKQIAETVHVATMMGLCNRVANAFGLESQDLLALTL"
     gene            complement(13426..15489)
                     /locus_tag="VHE33_13970"
     CDS             complement(13426..15489)
                     /locus_tag="VHE33_13970"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HVT98604.1"
                     /translation="MARKLRWVLAGLALLASAALSAQTASSPHEVAIPLGHSVAPLYH
                     GWKFSIGDSPLDPKTGAPLWAQPGFDDSSWENVDLTPKEGSENPITGTTGYVPGWTTR
                     GHAGYWGYAWYRIRLRIQSAPDTKLALAGPATVDDAYQLFDNGALLGSFGDFGHGRPG
                     IYYGQPMMFPLSPPPGASAIGSVHVIAFRMWMQPQTLFQASGVGGFEAAPSIGESSAV
                     AAQYQVQFDELIRAYLWQPLTVVVFGLLGLIALSLTLADRSDHVYLWIGALLLLISVD
                     AASGVLCVWTVWVHANYDHFSHDILLFSLQYAGWVMVWRSWFRQRRPAWIPWALIPLV
                     LILMASRTIEENFLFVVSQPVHNIAHAVSLLVRFTLSAFLFFIVVKGIREQGLEGWFV
                     LPAVLLAAAAEFTLELQNMGLQASWFPFHVQITLAVASQLLLVVVLAVLLVRRLVLSL
                     RSQRQMALDVKQAQEVQQVILPEATTAIPGFAIESEYRPAREVGGDFFQIIPWPADDS
                     LLVVAGDVAGKGLQAGMLVALLVGAIRSTAELNSEPEFLLQALNRRLLGRGDARATCL
                     ALRITRDGNVAVANAGHMPPYLNGSLVEIEGSLPLGVVEDLECSTMQLRMAENDRLLL
                     VSDGVAEAMNAEGELFGFDRVLDLVRSQPAAASIAETAQAFGQRDDISVISVTRIPVA
                     EPAMA"
     gene            complement(15573..16760)
                     /gene="ribB"
                     /locus_tag="VHE33_13975"
     CDS             complement(15573..16760)
                     /gene="ribB"
                     /locus_tag="VHE33_13975"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HVT98605.1"
                     /translation="MHDPHVMTDVPGALEEVKAGRMIVVVDDEDRENEGDLMLAAEHI
                     TPEAITFMAREGRGLICLALTEERADYLRLGPMTQENSSRFGTAFTESIEAREGVTTG
                     ISAYDRSHTIQVAIDPRSTAHDLARPGHVFPLRARKGGVLVRAGQTEASVDLARLAGL
                     IPAGVICEVMKDDGRMARVPDLIEFCHRHGIKMLTVAELIRYRLQHERYIVRVAESPM
                     PTEFGDFLMIAYESEVHGGQSHIALVRGDVHQSPTGSEPPVLVRVHSHCLAGDVFGTT
                     LCDCHRTLRQSLRMIAEAGRGALIYLHNTSRGFDIDRTATPHRIVFHREARARERHED
                     RHQRTLREVGLGGQILADLGIHRIRLLSNTPTHIPALQGFAVEIVEQVPIPIAEPARP
                     LSS"
     gene            complement(17011..18129)
                     /locus_tag="VHE33_13980"
     CDS             complement(17011..18129)
                     /locus_tag="VHE33_13980"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HVT98606.1"
                     /translation="MNARTLKAACLVLTSAGLIFLSAGCKKIEPLKLDCNASAPSIYP
                     GDAQTVTATPGQVAQKKNWDVVYTWSGTGVTGNGTTATVNTASLEPGNYTVKAEAKEG
                     KKGKEGTKPNTTASCSSDFTVKPFEPPTVSCSANPTSVMTGTSSTITATGSSPQNRPL
                     TYSFSASGGSINGSGNTATLSTTGAPAGNITVTCSVQDDKSHNVSATTSVEVQVPPPP
                     PPPKSQTLCSIQFTDKRRPGRVTNEAKACLDDVALNAQQKADATIVVVGNSAPLPEKK
                     GRHHKKEMTAADLAAARAVDTKDYLVNDKGIDASRIQVKTGSGGTDEVQDYLVPSGAN
                     FDNDVPGTTAVDEGAVKAQPRNPKPHHHHKKAMEGSAK"
     gene            complement(18609..19322)
                     /locus_tag="VHE33_13985"
     CDS             complement(18609..19322)
                     /locus_tag="VHE33_13985"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HVT98607.1"
                     /translation="MPIFSQHVGPTSSAWAYISGSGYQLPWIMLRAVIFDYGEVLCTQ
                     DHAAHQRLIALTGLHHDTFESLYWRDRHSYDLGRFDGRGFWAQFASDAGLTFTSEQID
                     ALIETDVVMWTSISEAMLAWARSLPRTGILIAILSNMVQEVLDTMRQKFSWLAEFNQL
                     TWSCELGIAKPDPAIYTLTCEKLGVAPADALFLDDRLVNVRAAEQLGLMALQFRSIEQ
                     LSRDLASPTFDGLPKPVPV"
     gene            19323..20522
                     /locus_tag="VHE33_13990"
     CDS             19323..20522
                     /locus_tag="VHE33_13990"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HVT98608.1"
                     /translation="MRFSSRTAWDLAETQLARALRERRASGREVLDLTASNPTQCGFA
                     YDEAGILAALGDRAALTYDPNPRGMRHAREAVCRYYAERDAQVEPEQVFLTTGTSEGY
                     GWLFRLLCDADDEVLIAAPSYPLFDFLAQIEDVRLVSYPLVYDHGWQIDFPGLREQIT
                     PRTRAIAVVHPNNPTGHYTRDAERRELEQICAERKLALIVDEVFLDFPLTSAAKTRQD
                     GAPRSFAAAEHSCLTFVLSGISKIAALPQMKVAWIVACGPEPERGEALERLEVIADTF
                     LSMNGPVQCALPAWLAGSAAIQEQIRARTATNLQNLDHTLLRYPVVTRLEVEAGWYAV
                     LRVPALGNDEDLAVRLVEECGVYVHPGSFFGFAGEGWLVLSLLTPEEEFRRGAERICE
                     HFKARGD"
     gene            20526..22775
                     /locus_tag="VHE33_13995"
     CDS             20526..22775
                     /locus_tag="VHE33_13995"
                     /inference="COORDINATES: protein motif:HMM:NF015751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HVT98609.1"
                     /translation="MQIPIKRTVERIPGGFMLVPLLAGAVAHTFVPNAGPFFGSFTGA
                     LFTGALPILAVFYVCVGSSIPVRALGQVVKRGGALLTTKIALGIAAGLILGHFLGNDP
                     VRAGWFAGVSTLAVVAAFNDTNGGLYMALMNQFGTAEETAAYSVMGLESGPFLTMVTL
                     GVAGLATFPWPTLLGAVLPMLVGLVVGNVDEEMREFFSRATPVMIPFFAFALGATLNL
                     KQVWAAGLVGIGLGLLVLLVSGAALIGVDRLCGGTGVAGVGAATTAGNAAAVPALVAA
                     ANHKYAEAAAPATVLVASSVIVTTMLAPLATAWWAARVRKKGDADAHKRGRVLILADD
                     LAGAADGAAACCATGREALLLLNGDGAVDEEVVVVDANTRCLGPEEASREMRRLVQCY
                     AGGGRVLFKKVDSTLRGHLAPELVAVLQARSEMLRDRPVAVFAPALPAQGRVVLDGEL
                     RVHGVPLEKTDAWKRERRGARSDVMAMLAGLGLRTTRLRLATVRSGASALKKAMASAA
                     QYAEVVICDAEMDADLAAIALASKVLGERAVWVGSSGLARHLPGPLRSSAQRAAAVPK
                     FAPEFALGPTLYMVGSMTEMAREQAERLGAVPGVETLRIPAAVLLAEAWAEELRRLEI
                     ALESRRDVLVVPQCDAPLNYEQGTELVQAMGRFVGGCADRVGGLVATGGETARAVLDA
                     WGVTRLRVRGEVEAGVAFSTTEGGRRAMPVVTKAGGFGDAQTLVRGAEFLRGLRRRAP
                     EASQAEASR"
     gene            22772..23770
                     /gene="pdxA"
                     /locus_tag="VHE33_14000"
     CDS             22772..23770
                     /gene="pdxA"
                     /locus_tag="VHE33_14000"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HVT98610.1"
                     /translation="MSLPLIAITMGDAAGVGPEVTMKTLAHAEVHAMCRPLVIGDAAR
                     LRAAGKIVGSGLEVRSVGEDSIATAAFDPGHVNCVDLGLVPADLEWGKLSALAGEAAY
                     RYIEVAARLALAGRVAAICTAPLNKEALWMAGHKFPGHTELLAHLTGTPEVSMMLTTP
                     KLRVLHVTTHIGLLDAIEKIEPGLVERTIARGQEALARIGIAKPRIAVCGINPHAGEH
                     GLFGRGEEETKLAPAIEKCRARGWNVEGPLPADTVFFRAQRGDFDLVIAMYHDQGHGP
                     VKVLGLESGVNITVGLPVIRTSVDHGTAFDIAGKGVADERSMVEALRQAVELAPQG"
     gene            23921..24628
                     /locus_tag="VHE33_14005"
     CDS             23921..24628
                     /locus_tag="VHE33_14005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT98611.1"
                     /translation="MEGRTAIVTGASQGIGAAIVDAFLGRGYDVVATSRHVSGAGYAA
                     SAHLALVDGDIGQAETAEQVARIAFDRFGSIDHVVNNAGIFRAKRFTEYTADDLRAFV
                     ATNLDGFVFITQLAVKQMLQQRTGGSVTTITASLADNPIAGVPGSIPMMTKGGLNAIT
                     LSLANEYAKDKIRFNAVAPGVVDTPMHAGKTAARSAMGTFSHPKDIADAVMYLTEAGH
                     VTGEVLHVDDGAHVGQW"
     gene            24625..25101
                     /locus_tag="VHE33_14010"
     CDS             24625..25101
                     /locus_tag="VHE33_14010"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HVT98612.1"
                     /translation="MRTAGSGAERRLAELGIELPAAPTPFGSYVETLQTGNLLFLSGM
                     LPVVDHKPKYLGRVGAELDAEKGRDAARTATLGALAAARKHLGSLDRVTRVVRLAVFM
                     ATSGDFFDQPRVADGASDLLRDVFGAEKLPVRSVLGVASLPLGMPVEVEVTFEVME"
     gene            25173..26216
                     /locus_tag="VHE33_14015"
     CDS             25173..26216
                     /locus_tag="VHE33_14015"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HVT98613.1"
                     /translation="MATAETAVTTMKAAQIGKPGAGFEIVECSIPTPGAGEVRIKVQA
                     CGVCHSDVLTVEGGFPGIQYPRVPGHEVAGVIDAVGAGVSAWRVGQRVGVGWHGGHDN
                     TCPECRRGDFNNCRNMQIAGISYDGGYQEYMLAPVEGLVSMPDSLSDVEAAPLLCAGV
                     TTYNALRHSGALPGDLVAVQGIGGLGHLGVQFANKFGYKVAAIGRGPGNAALAKKLGA
                     HVYIDSKATNAAQELQKLGGAKVILATAPSSKSMSELIDGLGPNGKFLVVGASTDPIE
                     VTPLQLIFGAKSIQGWASGVPMDSEDTLRFSELTGVRPMIETYPLEKAAEAYARMMSG
                     KAEFRVVLTMAAG"
     gene            26409..27269
                     /locus_tag="VHE33_14020"
     CDS             26409..27269
                     /locus_tag="VHE33_14020"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT98614.1"
                     /translation="MAAPIFYRTVKVDGLNIFYREAGPKDAPTILLLHGLPSSSRMFQ
                     PLLTRLADRYHLVAPDYPGFGHSDWPDPKQFAYTFDRIAVVMEDFTQALGLDHYAMYM
                     QDYGGPVGFRMALRHPERVEALIVQDAVAHNEGLGANWATRRAFWADRAKYEDALRTN
                     LLSLATTRTRHVGDDPNLEAYDPDLWSDEYAFLSAPGQAQIQSDLFYDYRTNVEAYPK
                     WQAWMQKTQPRLLVIWGKHDLSFDLGEPERYRKDVPKADVHVLDAGHFALDTKADEIA
                     GLVREFLGRK"
     gene            27386..28375
                     /gene="cyoE"
                     /locus_tag="VHE33_14025"
     CDS             27386..28375
                     /gene="cyoE"
                     /locus_tag="VHE33_14025"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HVT98615.1"
                     /translation="MALTPAASGVGPEIVPLDSHRGDGPHLHAPRAGETASLLVDYRE
                     LFKVRVTGLVVLTGWAGFYLGSMRSGISSLQPGLLETLLGIGAVSAGSAALNQAIERK
                     LDAKMHRTENRPMAAGRIGLVHGIGIGLLAIIAGTLWLTRETNFITGGLVLLTAFLYV
                     AVYTPLKRYTTLATFVGAFPGAMGPLLGWTAARGVIEWQGVALFAILFVWQFPHFMSI
                     SWLYREDYGRAGIRMLPVVQPDGWSTALEALVYAVLMIPASLLPFWLHMDGVAYAVVA
                     VVLGLGYLAYTIRFARIVRARSETESRMYARDLLKVSVIYLPLLLTALMLNAR"
     gene            28409..28981
                     /locus_tag="VHE33_14030"
     CDS             28409..28981
                     /locus_tag="VHE33_14030"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HVT98616.1"
                     /translation="MPTATTSSKLNPRPKTSAVVPVVALVGISVVAVLFLLWLIYVHP
                     ASDAAALHYTFLPNLNALLNGLAAIALLAGFYFIRTHRIAAHRASMMTAFVFSTLFLV
                     SYIANHALHGDTLYPVHNRVYYAAYLPILISHIVLSTVALPIVLITFYLSLSRRFAAH
                     RKLARYTFPLWLYVSVTGVVVRVMLVAAFR"
     gene            29004..29249
                     /locus_tag="VHE33_14035"
     CDS             29004..29249
                     /locus_tag="VHE33_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98617.1"
                     /translation="MQSEQPDDGTRALLKAGGGMLAAGIVAAVLTATVFGGISRHGPH
                     SNTGWLLLMVAMSCLPFGLMLFALGVAKWFRNRRNSQ"
     gene            29483..30439
                     /locus_tag="VHE33_14040"
     CDS             29483..30439
                     /locus_tag="VHE33_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98618.1"
                     /translation="MKFAGRFVHLAVFATCFALTPLLAQSNTPAQNPQSAQDQSQPVT
                     GVSHPPADDTIMADEDLPTPVPRPKPSAAIPITPVQPAATTAPALQHRNYEDDIVTSA
                     PASTSSASLSKHAWNPDEDIVSVVASNPNELASGTNVRVRLSTDLSTAETLRGQSFKA
                     IVDRDVYKDGRIIIPVGAEMRGRVVQVSQGHHIGPKATLRLRPESILLPDGTIYHIYA
                     EAVHSSAPGTRTDNEGGIQAAPHYKKDAVEYGAGAGAGAAAGALIAGPVGAGVGSLVG
                     AGAVTAHMIVGQPEAAKLPPGSVLTFSLTEPMELTPMRATAN"
     gene            complement(30436..30849)
                     /locus_tag="VHE33_14045"
     CDS             complement(30436..30849)
                     /locus_tag="VHE33_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98619.1"
                     /translation="MVPVTQGWSAMPNILTSWKEISQYLGKGVRTVQRWEREAGLPVR
                     RRDGAARHAVLAIPQELDEWARTRTRGPAGPVADSLRREIAILRDQTIELRKRIEFIE
                     ECGCAPVREKPRMTRAARTRGAERRFRSEATADAS"
     gene            31420..31653
                     /locus_tag="VHE33_14050"
     CDS             31420..31653
                     /locus_tag="VHE33_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98620.1"
                     /translation="MHYLRDGRIYVFDTAALSTRPGHKRERHLEHYWLCGVCAQRLTL
                     VQDGQGWVRILPKPALQGDEDDYLPSRHPEMAS"
     gene            31758..32573
                     /locus_tag="VHE33_14055"
     CDS             31758..32573
                     /locus_tag="VHE33_14055"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVT98621.1"
                     /translation="MHRLHIPAPPLASFVRCFWYWEGMPHPHARERLMPNGEATIVFN
                     LRDDEMRLYDADDLDRYTSCGLAGLTGPRTGCFAIDTAAQDRVIGIEFQPGGTFPFFR
                     EPAGEIANQSAPLDCLWGAAVGEIREQLLAARSVDAMFVILATALLERLVRPLELHPA
                     VGWARGWICRAPHVATVAGVVDRIGLSQRRFIELFRDQVGLPPKAFCRVRRFQRVLES
                     VHRKAGVDWAQVALDGGYYDQAHFIHDFQGFAGMTPAAYLARATEHLNHVPMG"
     gene            32647..>32912
                     /locus_tag="VHE33_14060"
     CDS             32647..>32912
                     /locus_tag="VHE33_14060"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT98622.1"
                     /translation="MSPVKPIPDGYHSVQPYLHVRGAANAIDFYKQVFGAEEVMRMPQ
                     PDGRIGHAEIRIGDSIVMLADEYPEKGIHGPAHYGGAPMTLMFYT"
BASE COUNT         6018 a         9039 c        11455 g         6400 t
ORIGIN      
        1 ccagggactc gcttctacaa cgtctatccg ctgtttcaca ccgcctcagt ctatgagggc
       61 ttccgccgtg attttggcga cagccgccgg gtcatgagcc tggccagggc ggcctatctt
      121 ggtgcccagc gcaatggcac cattttctgg tccagcgata tcgtttccac ctgggacatg
      181 ctgaagcgtt ccattcctgc gggtctcaac ttcaccgcca gcgggatgcc ttactgggat
      241 accgacatcg cgggcttttt ttcgccgcaa ctctcgtccg actaccacgc tgaacataag
      301 ccactgattg acgggtcgga tgtgcgcgac aacatcggca actacgaaga ctatccggaa
      361 ctcttcgtgc gctggttcga gtggggcgcg tttcaacccg tcatgcgcgc gcacggcgaa
      421 cgcatgcata acgaggtatg ggcctatggg aaacaagccg agcccattct cgcgaagtat
      481 ctgaaattgc gctaccagct gctgccgtac acctactctg ccgcctggaa gagctatcag
      541 accggcgccc cctacacgcg tgcactgttc atggatttcc cgaatgaccc gaaggtcaca
      601 gatattccgg acgaatatat gtacggcccg gcgctgcttg tcgctccggt ctcccaacag
      661 ggagccacca cccgttcggt ctatctgccg gccgggacgg attggtacaa cttctggaca
      721 aaagagaagt accacggcgg acagaccatc accgcgaatg cgcccatcga cactctgccc
      781 ctctttgtac gggccggatc gatcatcccg atcggcggtg ccgtcgagag cgcggcgcag
      841 aagcagacga ttgcgaaggt ggaggtgtgg ccgggcgcgg atgcagattt cgcgttgttc
      901 caggatgacg gcgaaaccta caattacgag aaatcgggcg gagacgtcac aaagctgcac
      961 tgggacgacc ggtcacagcg cctgacgcat accgggcacc ccgcgtggac gcagccggat
     1021 agtgaattgc tggaggtgat tcactagctt ccacaggcgg tcccggccat acatggcaag
     1081 gcgcccaacg cgaccgtggt gtgccgggcg ccggttgatc cgcttggtga gcagcacggc
     1141 agggaggctg gcttgaatga cctaactcgc ttgcggaaag cccggtacgg caaggctgtt
     1201 gtgtggctgt tcgcactcgg atttgggtgc agttcgcccg cccagacccg tgtcgttccg
     1261 tcgcgcctcg cacctggtca gtcgttgccg gcggaatcct ctgagtacag gcaagtgcag
     1321 cagcgcctgg cgcagggctg gaacacctgg gatgtgcaca gtgtgacgac acaggtgtta
     1381 ttgcctcagg gtctggcgat tcatacaagt ttgaagcaca acacgaccga gggcagcgac
     1441 gcgtttctgg gagatacgct gatcggaagg ctgacccctc acgccgagca ggttacgccg
     1501 gggccccacg cgtgggatgg cagctatacc gacctgcgcg tggcctggca tgggcacgaa
     1561 tggaggatgc agagcgcaca ggctgacggc gagctggtga tgctggcgac gccgctgtcg
     1621 ggcgcccagg gttccgctct tccccccacc atcatcttca gcgtggattt cctctggaac
     1681 cgcccaggta cgatcacagg tcacgacgat ttcatcgagg cgcagagtcc caccggaacc
     1741 gcgcgcgtct tctgcacctg tgcgccggct ggatcgaaga tgcgggacca gtggatcgac
     1801 agtccagtga gaggcccctt tttctccgcc gatttcacag gacccatcgg catcggcacg
     1861 gggaaacgcc ggaccctcga cgaaatcagg gtggctgtcg aaaagcagcg acaagcatac
     1921 gaagagtcca ttcacgccgc gggaagcaac gcacccattg tcgatgccat cgaaaccacg
     1981 cttggctggg acacgatcta cgaaccggca ggggagcggg ttgtgtcacc cgtcagccgc
     2041 gtgtggagcg ttgactgggg tggttatgtg atcttcgact gggacaattt cttcgctgca
     2101 acgatggcgg gaatcggcga ccgcgatctt gcctatgcca atgcgctgga aacactgcgc
     2161 ggagaaacga atcagggatt tgttcccaac tacgcccgcg gcggtggatg gaaaagtttc
     2221 gatcgatcgg aaccgccggt cggtgcggtg acagtgcttg gcctctaccg aaaatctcac
     2281 gaccgctggt tccttcgcga cgctttcgat cctctgctgc gctggaatcg ctggtgggcc
     2341 acgcaccgcg acatcgatgg ctacctggcg tggggcagtg atggcgacaa ttcccccggc
     2401 aatctcgacg atcagtcacg cggaacgcgc gtcggagccg ttctggagtc ggggctggat
     2461 aacagcccga tgtacgatac agccacctat aacgaacaga agcatgtcct ggagtatgcc
     2521 gacgttgggt taatgagcct gtacattgcc gactgcgacg ccctcgcgaa gatcgcgcag
     2581 gagttaggca tggcggagcc cgaaaaagaa ctcacagagc gtgcggcccg ctatcgtgca
     2641 aaactggcaa cgctctggaa cgatgagaaa ggcattttcc tgaacaagga tcttcatacc
     2701 gggcaatcca atacgcgcct gtcgcccaca aacttttacc ccatgcttgc ccacgcggcg
     2761 acaccggacc aggcgcacac gatgatcgca aaacatctga agaatccggc ggagttttgg
     2821 ggtcaatggg tgattccgtc tatcgaacgc gatgatcctg ccttccacga ccaaaactac
     2881 tggcgggggc ggatctgggg accgatgaac tacctggtgt atctgggcct ctgcaactac
     2941 gatgatgctg ccgtacgcca ggaattcgca cagaagtcct atgccctgtt tcttaaggag
     3001 tggacggagc atcgccatgt ccacgagaac tacaactcta tccttggcag cggcgacgat
     3061 gtgagcaata gcgaccgctt ctaccactgg ggtgctttgc ttggatatat cgaatatctt
     3121 gaagcgagcg gtgccgggaa gcgcccaccc agtggggatt gagcacttcc ccccaatcgg
     3181 gaaatggctg cccgccagga gccgttgtca tcccgaattc ggtgcgcgcc cgggagggtg
     3241 cgagggacca taggcctttg cttcgatccc tgcaaatccg cctggccggg caataggctc
     3301 agaacacccg tcaatgctgc cccagccctg atcatcgatt tgaaaaagca tgctcagtca
     3361 ggccgtgaat cggtttggga ttcccagtcc tcgtcgccag gcactctgta ccccttaccc
     3421 tgcccacctg taccctgaat tttgacgatg cccgatccca ctcccacctc gcctcgcgtt
     3481 cgcttcgcgc cctcgcccac cggctacctc cacgtcggcg gagcccgcac cgccctcttc
     3541 aactggctct tcgcccgcca ctttggcggc accttcatcc tccgcattga ggacaccgac
     3601 ttcgagcgct cctccgaagc catggttgag ggcatcctcg aaggcatgcg ctggatcggc
     3661 ctcgactggg acgaaggccc cttcttccag tcccagcgcc tccctctcta ccaggccacc
     3721 gcccaaaaac tcatcgactc cgggcacgcc tattactgct tttgcaccaa agaggaactc
     3781 gaagcccgcc gcgcccaggc cactgccgcc ggcggtcgcg cgatgtacga ccgccgctgc
     3841 cgttcgatct ctccggttga ggcaaagaag cgttgcctcg acggccactc cgcaccagcc
     3901 gttcgctttg ccgtccccga cggcgtcacc tcctggaatg acgccgtctt cggcaaagtt
     3961 gaatttgcca acgccgaact cgaagatttt gtcctcctcc gctccagcgg ggcccccaac
     4021 gacagccctt cgtcgttggg gtgtttggac ggtatcccca cctaccacct ctccgtcgtg
     4081 gccgacgaca tcgatatgcg cctcacccac atcattcgcg gcgccgatca catttcgaac
     4141 acacccaaac agatcctcca gtatcatgcg ctcggcgccg ccctgcccac cttcgcccac
     4201 gttccgctca tcctcggccc cgacaaaacg cgcctttcca aacgtcacgg tgcaacctcg
     4261 gtcatcgctt acaaagacat gggcatcgtt ccggaagctt tcctcaactt ccttgctctg
     4321 ctcggctggt cgccgcgttc ggcgcaaagc tcgaagcaaa atccgcagca gcaggaccgc
     4381 gaagtcttca catccgacaa cctcatccag ctcttcaccc ttgaaggcat ttcgaaatcc
     4441 aacgccgtct tcgacaacga caagctcgcc tggttcaaca ccgagtacat ccgctcctcg
     4501 gacccggata agttacttcc actgatacag aatgagtgga aggccgcggg attcgtgccc
     4561 acccgcactc cagccgaggt tcttgccgct atcgagttca tccggccccg cgcccgcagc
     4621 ctgaaagact tcgccaccct gttccgggct tatttcagcg acgatttcga atatgatccc
     4681 gccgctgtta caaaattcct ggccgacccg gcgcttccgg ggctccttca ggacttggct
     4741 catcgctatg ccgccgctcc ggacctcacc gaagcttctg ccgaagagat tctgcgcgcc
     4801 ctggccgccg agaaaggcct caaagccgga gcgctgatca acggcgcccg cgtcgcactc
     4861 accggccagg ccgtcgcccc ctcgctcttc gccgttatgg ccctgctcgg caaagatcgc
     4921 gtggtccgcc gcctgtctgc agtcgatcgc gtgcccacct caggttgatc gcccgtcgca
     4981 ggtcagccgc gtggcccatg tctgcccggt accccgtccc tgcccggttt tggcacgttg
     5041 cgggaagcgc tatccgctct ctcttgatac aatcgatgga gcggaatggt gacagttccg
     5101 cctttcgttc gacccggttg cgcgtccggc attctcaatg cagacgcaga ccaaaaacgc
     5161 gaagtgcaac gcttcgcgag cagtttccaa aaggcaaggg gacgtagttc agttggttag
     5221 aatgctgccc tgtcacggca gaggtcgcgg gttcgagccc cgtcgtcccc gccataaacc
     5281 agagaaaaat cacaggttta tggctctccc cctcaacaaa atttaggttc gatcccgcga
     5341 gcaatttgtt tgaaggcact tgcgaaaggc gttcgctgtt tgctgaggga tgaacctgtc
     5401 cttcgattgg ctgggtctgc cggctggcag ttgcccggca ggttgcgggg cgggggcttg
     5461 caggaaagca ggagcccgca cgctgaggtg cccccgatct tttccagctg ggttcggatt
     5521 gatcgaacgg ggaacgttca aggcccgaac cgattccggg cacccgccgc agcgctttca
     5581 tcctgtccaa ttttctgccg atgagccgta cagcttccct ggggcagccg gaggagaggc
     5641 gttgccgggg attggggggc cgttgcgcgg cgagacgttg ggtgcgatga ccgatgatgg
     5701 ggttcagagc gggcggtcgc tcaaggaact cctcgccatc gaggaggcgc tgcgagtcag
     5761 cgagcagcgt tatcgagttg cgtttgagac gagcctggat gcgatcggca tcacgcggat
     5821 cgatgacggg atgttcgtgg atgtgaaccg gcagtttttc acgatggtgg gttatacgcg
     5881 ggaagaactg gtggggcaga cgtcgctgca ggggtgcacg tggaccgaca gcgaggggga
     5941 gagccatccg gcagaattca tcgacgtaac cgggcgctcg acgctggaca tcgggctgtg
     6001 ggaggattca gcgcagcgag ccgaactgat ggcgatcctg cggcgagaag cggtttgccg
     6061 gggattcgag gcgcgattca gaaggaaaaa cggggagttg ttctgggtcc agctgtcggc
     6121 atcggtaatt cagctggacg gagtggattg cgtcctgttt gtgacgcggg acatcacggc
     6181 ggcgaaggcg gcggaagagg agatccgcaa cctgtcgctg tatgacgatg tgacgtgcct
     6241 gccgaatcgc cggcatttgc tggagcggct gcaagcccgc cagcagaccg cgatgggaaa
     6301 cgcaaagagt gcgctgctga gcatcagcct ggaccacttt cgaaccttca atgattcgtt
     6361 cggtggtgcg gcgggagagc tgctgctgcg cgagatggcg cggcgggtgg agacctgcgt
     6421 tcgcgaatgc gatacggtgg cgcggtcggg gggcgccgag tttgccgtgc tgttgggaca
     6481 cctgtgcgga tcgagggagg acgctgcggg gcaggcccag gagattgcgc gccgagtgct
     6541 ggaacggatc agcgagccgt gcggggtggc gggacagata tgccactgca ccgcgagtat
     6601 cggaatcaca atctttcagg agaggggact cagcggggag cagatcgttc agcagagcga
     6661 cattgcggtg gatcatgcgc gggtggcggg gcggaacacg atccgcttct tcgccccggc
     6721 gttgcagacg gcgatcagcg agcgcgtggc gatggaggcc gatctgcgcc gggccatgac
     6781 cagcaacgaa ctaatgctct gctaccagcc gcagatggat cggggaaatc tgacgggagc
     6841 cgaggcgctg gtgcggtggc gccacccgac gcgcgggctg ctgctgccga gcgccttcat
     6901 tccggtggcg gaggaagcgg ggttgattgt gccgctgggg gaatgggttc tggagaatgc
     6961 ctgccgccag ctggcgcgct ggtcgcacag cgggaagatg cgggatctga cgctggcggt
     7021 gaacatcagc gcgtggcagt tccggcagcc ggattttacg gagaaggtcc tcgacacgat
     7081 tcgcaggcac caggtcgatc cgcaaaggct ggaactggag ctgacggaga gttcgctggt
     7141 ggaagacgtc gatgcggtgg tgatgcggat gggggagctg aaggcgcatg ggctgcgatt
     7201 ctccgtagat gatttcggag tgggctattc atcgctgtcg tatctgcagc ggcttcccct
     7261 cgacaaactg aagatcgaca tgcagttcgt gcgcaacctg ctgatcgatc cgggcagcag
     7321 ggcgattgcg cgggcgatca tttcgctgtc gcaggctctg gggctgcggg tcattgcgga
     7381 gggcgtggag tcggaacagc agcggaagca cctgtccgag cttggctgcg atgcgtggca
     7441 gggttttttg tgcagccagc cggtggttgc ggaggaattc gaggaactcg ttgccggcga
     7501 gaaagcacag cggctcccgg cgcggaccgg gagccgttga tggtcgaatg atctagggga
     7561 gggtgaccgt agcggcctga ctccagactg tgccgccggt attgacggca aagactgtga
     7621 tcgtgtgctg gccgtgcggg acctggaccg cggcccagag cgtggttgaa ttggtttcgg
     7681 tgtattgctt ggtggagtcg acccagatct ccatccgggc gagggttccg gtgacggtgg
     7741 ctgttgcgcc tacgttgacc gggcccgcgg gctggtttga gttcgtggaa ggggagcaga
     7801 ggtggacgcc ggccgaggtt ggggcagcgc aggtggaggg gatgttgaag gtgaagttcg
     7861 attgctggag ccggttgtcg atgtcgacgg caaagtacgt tccctgatgg gagccggcag
     7921 cgagcgtgga actgaagttg aaccaggcgt tgctgccgcc gttgtcgccc caggtgtggt
     7981 actcctcgcc gagtttattg ccgtcgaccc agagctcgat tttgcgcagt ttattcaggg
     8041 agtgggcggt ggcgttgaag ttgaccgtgg ttccggaaga ggcgacgggc gagcagttca
     8101 tgatgccgcg agcggacgtc ggatagctgc agttgctcca gaggccgacg gtgctggtgt
     8161 tgataccggt gtcgacggtg gaggttccgg cggatggcgc aacgtagcgc gcgacgacaa
     8221 agtcgggccg ggtgtcgtgg ttgaagtcgc cggcgacggg agcggtaatg tagccgttgg
     8281 gattctgggg gggaccgcct ccaggaggca aactccaggt ttgggtggtg aatgcgccgc
     8341 ggtcggcttc gccgcgcaag aagaccatat agctggtgct ggtgaacagc aggtccatgt
     8401 ggccatcgtc gttgaagtcg gctacggcgg gtgcgccggc gtaatacgct gaggggatcg
     8461 cggtggaatg ctgatcgaat ccgcgccccc attcgccgta gaaggttttc aggacccagg
     8521 cgtcggtgtc gaagacgtaa aagtcgctga agccgtcgct gtcgaggtcg gccgcgccga
     8581 cctggaactc gccgccgggc acgctgtagg gagtggtatc gatcaggtcg aagttgccct
     8641 cgccgtagag gacatggacc gcggtgttgt cgatattgcc ggtactgccg ttgtaggtga
     8701 tgacggaggc gacgatgtcg gcattgtcat cgccgtcaaa gtctccgaca ctcatgctga
     8761 taatgggatc gccgaagccg gtttgcgtgg gcgcggtgag aaccggatgc gcagcctgga
     8821 agaccccgtt gccgcctccg ggcagcacat agagagtgct gttggaactg tcgctggcag
     8881 tgagggccag gtcggcttcg ccgtgccggc cgaagtcggc ggagacgatg gcattgttat
     8941 ccagggtttg tccggacgcg agggtcaggt tgaaatacca gggattgacg aaggtgccgt
     9001 cgcctttgcc gagatagatc gccaggacag tcgttcctgc gaccggcatg gcaatgtcga
     9061 ggtgcccgtc attgttgaag tcgccgaagg ccatgggagc gaaggcgacg gtgggaggat
     9121 agttcaccgc gaccggagcc ttgaaggttc catcaccgtt ggcaatgctt acggcgaact
     9181 gaccatttcc gtacgtggcg tcgtaagaga cttcgtcctg gaccagatcg gggacgccat
     9241 cgttgttcag gtcgacggcg taaatctttc ggggagtggg gctggaactg tcggtggaac
     9301 taacaaaaat gttgttgggg ccggtgatga ccgagaagct gttattgggc tgggcgaagc
     9361 acgtggctgc gaaagccatc gtgcagagga cggctgcgag cactttcctg ctgtgcgaca
     9421 cggaggactc ctgaaaaatg ccgtaggaag agggagccga ctttcaggcg tgcatctgct
     9481 tagagggggg cgacgatgag ttgagttgcc gtggagcgcg gtgagatccg aaattttcgc
     9541 aaaggtttct cggtggtttc ggatactttg cgccggtgaa ctgccaggtt cacgccatgg
     9601 cggctcctcc tcctcccttg gttgtagaga agaaccgttc catcagcgga cgacgctcga
     9661 taggttccgc cagtactatc ccgcgtaaga ctcaccccag caaaaacaca aacaagagtc
     9721 ctgcttctcc ctgcattgtc tgactggagg ccatcgtgca tagatttgtt ttgagccgct
     9781 ggtgcggctc ccttctgttt gtgctcctga ctgccgtgac gagtctggcg cagcaactcg
     9841 caaccggcag catccgcgga accgtgatcg acgcctccgg ccggccggtg gcccacgcac
     9901 aggtcacact gaaaaacgat cgccagggaa ctgagcggtc cttttccacg caaaacgacg
     9961 gcgaattcac ctttgagacg ctggaggctg cgaactacac gcttacggcg accggtacgg
    10021 gtggatttgc ggaatggcgc gagccgttga cccttgacgt tggccagatg ctttcgatcc
    10081 cggttcattt ggtggttgcc ggcgccccaa caacggtgca ggtatcggcc gatcggcagc
    10141 gtgtggacac gacggattcg gtagtgggcg gggtgatcga gtctcgccag atcgagacgc
    10201 tgccgctcaa cggcagaaat tatctggagc tgtcgtatct ggttccgggc aatgcaccgg
    10261 cgccgaactt cgatcccacc aaggggaata ccgtcgttgt ttcgtcggca ggacaaacgg
    10321 gccggggggg caacgtaacc atcgatggcg cggataacaa cgacgatgtg gtgggcggat
    10381 cgctggtcaa cattccggaa gatgcggtgc aggaatttca gatagcgacc aaccggttct
    10441 ccgccgcgct gggacgttcc ggctcaaccg tcgtcaatgt cgttacgaag cagggttcca
    10501 acgcgttaca tggcggaatc ggcttttatg aacgggaccg ggttctgcag gcgcttcccg
    10561 cgacgtacga tcccacgatt ggcagcacgc cgccgtttca tcggcagcag tacgcaggag
    10621 aagtaggcgg gcccattcgc cacgacaaag cgtggtggtt tgtcgccatg gaggatcggc
    10681 aacaacaggg agcagacctg gtgggggtgc gggattcctc cagcaggacg atccatcggc
    10741 agttcgcgac cgccccgctg cacgattatc tgaccacgga acgggtggac tttcagatga
    10801 cgccgcggga tcgggtgggg gtccgcgaat ctctggaact ggaagacgat ctgagccaga
    10861 gcacgctgga ccgcgcgctg gcgacggcgg cgtatcggca gacggcggtg aatcatctgc
    10921 agggactagc ggcggactgg gtgcacatct ttacgccggc ggtgatcaac cgcatgagtt
    10981 tcgccgacaa caacttcatg aatacgacgg cgccgacttc cacagcgcca cagatcacgt
    11041 atcccgatct ggacgatggc gcaacctatc gcgtcccgca aaagacgaag caattccgct
    11101 tgcagggcga cgatgtgctg agctggtcgc ggcacaatca tgcgctcagc ctcggcggcg
    11161 aggtgcagtg gatcgatgcc gacttttacc tcggggtgtt ccagcagggg aacattcaga
    11221 cggtcgagga ttttccggat ttcgatcgca accaggacgg caaggtcgat gatgacgatc
    11281 tgttgttcgc ggtggcgttg cgcagctcga caccgcagcg tgccctcgtg ctgccgaata
    11341 atgacaacta tcatctggcg ggattttttg aagacgactg gagggttcat ccacggctca
    11401 cgctgaatgc tggcctgcgc tgggagatgg atacgaacct gaacaacctg agctggtacg
    11461 agaaccggaa tccgatcgtg cagtccttct acagtggcac gcggcaccgc gatttcaatg
    11521 attggggacc tcgtttcgga tttaactatg ccttcaggcc gaacctcagc ctgcatggcg
    11581 gctacggaat ctattacgac cgcatcacgc tggaggtgat gtcgctggag aagggattcg
    11641 acggccgcgc actggcgctg aacgtgacgg cgggcaacgt gatcaccgat ccgggcggca
    11701 atccagtcta tctgaatcct gatggaacgt ttgcgccggg agccccgcag gtattgtcga
    11761 atccgtttgg tggtttcatt ttcaccgggg ccggagcggc ggggatcgac gtgatcaaca
    11821 accgcatgcg caatccgatg gtgcagcagt tcaatctcgg gatcgagaaa gaatttagcc
    11881 ggggactatt cgtccaggtg gacggcgttc acaatctggg gacgcggttc atcatcgggc
    11941 ggcccgtggg atcggtattc aactcggaaa cgcagggccc ggatggagtc acggatctgg
    12001 agtcggccgt gaacacgaag tacgacgggc tgtgggtcag cgttcgccag catctggcgc
    12061 gttacggcga attcgacgca gcatacacgc tggcgaaggc gtacaactac gccaacgacg
    12121 accagattcc gttcgagtat tcgccggtcg atcccaacga tctgcatcgc gagtatgggc
    12181 cgccgccgaa cgaccagcgg cagcgcctgg tgctttccgg cgtgggaaat ctgccctgga
    12241 agctgcgctt tgcgccgatc tggaccatcg cctccggcgt cccgatggat attcttctcc
    12301 ccgatgcgag cgaaagggtt ccgacgctgc agcggaatgc cggggggcgt gagttccaca
    12361 atgcgcagca actgaatgcg tggatttcga agacgaatgc ggccggtggc gtgtatgaag
    12421 ccagcgccaa cggctatgta atgctccccc aggtcagcaa caaggcgcgc tttaacgaca
    12481 gcttcaatgc gatggatttc aggctggatc gcaccgtatc gtttggggat cggtacagcc
    12541 ttgatctgat cggggaagcc ttcaacattt tcaacgtgac gaatattctt ggagtcagca
    12601 acctcaacta ctcggggtac gcgaacacgc tttcgccgga ttcggggaat ccgagttttt
    12661 cctccagttt cgggatgccg gcttcgaccg cgggcggcgt ctttggatcg ggagggccgc
    12721 gcgcctttca gtttgcggcg aaactggcgt tctgatcact ctgtgcccgc cggaggtaaa
    12781 cctgtgagtc gcccggagta cgaactgctg gcacggctgc ggccctcgcc agtggaacag
    12841 tcggagacga agatcgcgat ggtggcggag tctgaagcag aaggtgaaac agctgctgtc
    12901 taccagtact ttcgcgatca aaccgggcgg caggacgttc ccggcattct gaagtgtttt
    12961 gcgacggatc ccgggtacgc ccggcacatg gtggacttgt cgtcgtcgct gttgttcagc
    13021 gacggcaagc tgagccgccg ccacaaggag atgattgcga cctgggtctc gagcctgaat
    13081 cgctgtccgt actgtttcga cagccacggc ttctttcttc aggtgcaggg cgggtctgat
    13141 gctctgctgc gcagctttgc agagcgcgat ctgaacggtg ctgcgctgac cgatgctgag
    13201 cagaagctgc ttgcctatgt agagaaggtg aatggcgcgt cgttccggac cagccgcgag
    13261 gatgtggagt ccctgatcgc agccggctgg agtgaaaagc agatcgccga gaccgtgcac
    13321 gtggccacca tgatgggttt gtgcaatcgc gtcgcgaatg catttgggct ggaatcgcag
    13381 gacctgctgg cgctcacgct gtgaaggaaa cgcgcgacgc caggttcacg ccatggccgg
    13441 ttcggcgacg ggaatgcggg tcacggagat gacgctgatg tcgtcgcgct gaccgaaagc
    13501 ctgggcggtc tccgcaatgg aagcagccgc gggctgagat cgaaccagat cgagcacacg
    13561 atcgaagccg aacagctcgc cttcggcatt catcgcttcg gcgacaccat cggagaccag
    13621 taacaggcgg tcgttttccg ccatgcggag ttgcatcgtg gagcactcca gatcttcgac
    13681 gacgccgagg ggcagggagc cttcgatctc gaccaggctt ccattgaggt aaggcggcat
    13741 gtgtccggcg ttggcgacgg cgacattgcc gtcgcgtgtg atgcggaggg caaggcaggt
    13801 ggcgcgggca tcgccgcggc cgaggagccg gcggttgagg gcctgcagca aaaattcggg
    13861 ctcggaattc agttccgcgg tactgcggat ggcgccgacg aggagggcga ccagcatgcc
    13921 ggcctgcagg cctttgccgg ccacgtcgcc ggcgacgacg aggaggctgt catcggcggg
    13981 ccagggaatg atctggaaga agtcgccgcc aacttcgcgc gccgggcggt actcactctc
    14041 gatggcgaag ccggggatgg cggtggtggc ttcgggaaga atgacctgct gcacttcctg
    14101 agcctgtttg acgtcgagag ccatttgccg ctggctgcgc agagagagca caagacggcg
    14161 gacgagcagc acggcgagga cgacaacgag gaggagctgc gatgccactg ccagggtgat
    14221 ctggacatgg aagggaaacc agctggcctg gagtcccatg ttctgcaact ccagagtaaa
    14281 ttcagcagcg gccgcgagga gaaccgcagg aagcacgaac catccttcaa gaccctgctc
    14341 gcggatgccc ttgaccacga tgaagaagag gaaagcggag agagtgaagc gcaccagcag
    14401 ggagaccgca tgtgcgatgt tgtggaccgg ttgggagacc acgaagagga agttctcttc
    14461 gatggttctg gaggccatca ggatgaggac gaggggaatg agggcccacg ggatccaggc
    14521 gggacggcgc tggcgaaacc agcttctcca aaccataacc cagccggcgt actggagaga
    14581 gaacagcagg atgtcgtgcg agaagtggtc gtagttggcg tggacccaca ccgtccagac
    14641 acagaggacg ccggaggcgg catccaccga gatgagcagg agaagggcgc cgatccagag
    14701 atagacgtga tcagagcgat cggcgagggt gaggctgaga gcgatgaggc cgaggagtcc
    14761 aaagaccacg acggtgagcg gttgccagag ataagcgcgg atgagctcat cgaactgcac
    14821 ctgatattgc gcggcaacgg cggaggattc gccaatcgag ggtgcggctt cgaaaccgcc
    14881 cactccgctg gcctgaaaga gggtctgggg ctgcatccac atgcggaagg cgataacgtg
    14941 gacggagccg atggcggagg cgccgggagg cggagacagg gggaacatca tgggctgtcc
    15001 ataatagata ccgggacggc cgtggccgaa gtcaccgaag gatccgagca gcgcgccgtt
    15061 gtcgaacagc tggtaggcat cgtcgacggt ggcgggtccg gcgagcgcga gcttcgtgtc
    15121 gggagcagac tggatgcgga ggcggatgcg ataccaggcg tagccccagt atccggcgtg
    15181 tccgcgggtg gtccagccgg gaacataccc ggtggttccg gtgatgggat tttcggagcc
    15241 ttctttcgga gtcaggtcga cgttctccca ggaggagtcg tcgaagccgg gctgagccca
    15301 gagcggtgcg ccggtcttcg ggtcgagggg cgagtcgcct atcgagaact tccagccgtg
    15361 atagagcggc gcgacggaat gaccgagggg gatagcgact tcgtgcgggg acgaggccgt
    15421 ttgcgcgctc agtgcggcgg aggccagcag agccagacca gcgagcaccc agcggagctt
    15481 tcgcgccatt ccactgacct cagggattca tcgaactgat tgcaacacta tatatcccga
    15541 aagtggaacg tcgtctggag gccacccaga tttcaggacg agaggggccg ggcgggttcg
    15601 gcgatgggaa tggggacctg ctcgacgatt tcaacggcga aaccctggag cgcggggata
    15661 tgcgtggggg tattgctcag caggcggatg cggtggatgc cgaggtcggc gaggatctgg
    15721 ccgccgagtc cgacttcgcg cagggtgcgc tggtggcggt cttcgtggcg ttcgcgcgcg
    15781 cgggcctcgc gatggaagac gatccggtgc ggggtggcgg tgcggtcgat gtcgaaaccg
    15841 cggctggtgt tgtgcaggta gatcagagcg ccgcgtcccg cttcggcgat catgcgaagc
    15901 gactggcgca gggtgcgatg gcagtcgcag agggtggtgc cgaagacgtc gccggcgagg
    15961 cagtgggagt ggacgcggac gaggacgggc ggctcggatc cggtggggct ctggtggacg
    16021 tcgccgcgca ccagcgcgat gtggctctgg ccgccgtgga cttcgctctc gtacgcaatc
    16081 atgaggaagt ctccgaactc ggtgggcatg gggctttcgg cgacgcggac gatgtaacgc
    16141 tcgtgctgga gacggtagcg gatgagctcg gccacggtga gcattttgat accgtgacgg
    16201 tgacaaaatt caatgagatc ggggacgcgc gccatacggc cgtcgtcctt catgacttcg
    16261 cagatgactc cggcggggat gaggccggcg agccgcgcga ggtcgacgga ggcttcggtt
    16321 tggccggcgc ggacgagcac tccccctttg cgcgcgcgca gcgggaagac gtggccgggg
    16381 cgggcgaggt cgtgcgcggt ggaacgcgga tcgatggcga cctggatggt gtgggagcgg
    16441 tcgtacgcgg agatgccggt ggtgacgcct tcccgggctt cgatggattc ggtgaacgcg
    16501 gtgccgaagc gcgaggagtt ttcctgggtc atgggcccga ggcggaggta gtcggcgcgc
    16561 tcctctgtga gggcgaggca gataaggccg cggccttcgc gggccatgaa ggtgatggcc
    16621 tcgggagtga tgtgctcggc ggccagcatg aggtcgcctt cgttctcgcg atcctcgtcg
    16681 tcgacaacga cgatcatgcg tcccgctttg acttcttcga gggcgccagg gacgtcggtc
    16741 atcacgtggg ggtcgtgcat ggatctattg tctgatacag agatgcctga gggccagtcg
    16801 gcgatgcgac ggtaaggggc gttggggaag ggacctattg agttggttag gaacgcttgt
    16861 ccgggtccct gagaggattt gctgcctgct atgagataaa gcttagcctg gggtaatttg
    16921 ggcgcgctga tcgttcttcg gccggtttgg catttggcaa aaaaggccgg tccttttgag
    16981 accggccttt cgctttgagt tagatggagg ctacttggcg ctgccttcca tggccttctt
    17041 gtggtgatgg tgcggttttg ggttgcgcgg ctgtgctttc actgcgcctt catcgaccgc
    17101 ggtcgtgccg ggcacgtcgt tgtcgaagtt ggcgccggaa ggaacgaggt agtcctgcac
    17161 ctcatcggtg ccgccggagc cggttttgac ctggatgcgg ctggcgtcga tgcccttgtc
    17221 gttgaccagg tagtccttgg tatcgacggc gcgcgcagca gccagatcgg cagcggtcat
    17281 ctctttcttg tgatgacggc ctttcttctc aggcagcgga gcggagttgc cgacgacgac
    17341 gatggtagcg tctgcttttt gctgtgcgtt cagcgcgacg tcatcgaggc aggccttggc
    17401 ttcgttggtg acgcggccgg gccggcgctt gtcggtgaat tgaatcgagc agagagtctg
    17461 cgacttgggc ggcggcggag gcgggggaac ctgaacctca accgacgttg tcgccgaaac
    17521 gttatgggat ttgtcgtcct gcaccgagca ggtgacggtg atgttgcccg ccggcgcgcc
    17581 ggtggtggac agggttgccg tgttgccgct gccgttgatg gatccgccgg aggccgagaa
    17641 gctgtaagtg agcgggcgat tctgcgggct cgatccggtt gcggtaatgg tggaactggt
    17701 gcccgtcatg accgatgtgg gattagccga gcagcttacc gtggggggct caaagggctt
    17761 gacggtgaaa tccgaggagc aggaggcggt ggtgttgggc ttggtgccct ccttgccctt
    17821 cttgccttct ttcgcttcgg ctttgaccgt gtagttgccc ggctcaagcg aagcggtgtt
    17881 gacggtggcg gtggtgccgt tgccggtgac accggtgccg ctccaggtgt agaccacgtc
    17941 ccagtttttc ttctgggcga cctggccggg tgtcgcggtt acggtttgcg cgtcgccggg
    18001 gtagatcgat ggagcgctgg cgttgcagtc cagcttcaga ggctcgatct ttttgcaacc
    18061 cgccgagagg aagatcaggc ctgctgaagt gagaaccaga caagctgctt tgagagttcg
    18121 tgcattcata gatgattttc tccgcattcg aaaacgttca tgtcatccgg tgatccgctc
    18181 gtgcgagtgc aggttacaaa tacggcagaa acccgtttcg ggcggcgatg cgaaaagcgg
    18241 cggctgcgag gagccgcgac gtccgtccaa cagtgtagct ccggtcgagc catctgcaat
    18301 gcgcaatcgc gcccgcattc gggtaccaca gtgctcgctg gattcggcgg aacaagagcg
    18361 ttgagtgccg agcgcgcgca tctgcagttc agttaagcgg tcgcggcaga gcgataacgt
    18421 tactaatcag gtaatatgtc agatttgtgg acgttataaa ttgcgggttc cccgcagtca
    18481 ggccgcgaga taactcagtc gttactatcg gaagattgcc ccccgccccg ggcggccagg
    18541 aggggccggt aacgacagtc cgcggggagg aaagcggctt cagaggccgg ctgggaacga
    18601 aaaatctctc ataccgggac cggtttgggc aggccgtcga aggtgggtga tgccagatcg
    18661 cggctgagct gctcgatgga gcggaactgg agagccatga ggccgagctg ttcggcggcg
    18721 cgcacgttca cgagtctgtc gtcgaggaag agggcatcgg cgggtgcgac gccgagtttc
    18781 tcgcaggtga gggtgtaaat cgcggggtcg ggtttggcga tgccgagttc gcaggaccag
    18841 gtgagctggt tgaactcggc aagccaggag aatttctgcc gcatcgtgtc caggacttcc
    18901 tggaccatgt tggacaggat ggcgatgagg atgcctgtcc gcggcaggct ccgcgcccag
    18961 gccagcatcg cctcgctgat tgaggtccac atgaccacgt cggtctcgat gagcgcgtcg
    19021 atttgttctg aggtgaaggt gaggcccgca tcggaggcga actgggccca gaagccgcgg
    19081 ccatcgaaac ggccgaggtc gtagctgtgg cggtcgcgcc agtacaagct ctcgaaggtg
    19141 tcgtgatgaa ggcctgtaag ggcgatgagg cgctggtggg cggcgtgatc ctgagtgcag
    19201 aggacctcac cgtaatcaaa gatgacggca cggagcatga tccatggtag ctggtagccc
    19261 gagcctgaga tgtaggccca ggccgagctg gtgggcccca catgttgtga aaagatgggc
    19321 acgtgcgatt ttcctctcga acggcctggg atctggctga gacgcagctg gcgcgggcgc
    19381 tgcgcgagcg gcgagcctcg ggacgcgagg tgctcgatct gacggcgtcg aatccgacgc
    19441 agtgcggatt tgcgtacgac gaggcgggaa tcctggccgc gctgggggat cgcgcggcgc
    19501 tgacctatga tccgaatccg cggggaatgc ggcatgcgcg cgaggcggtg tgccgctact
    19561 atgcagagcg tgatgcacaa gtcgaaccag agcaggtttt tctgacgacg ggcaccagcg
    19621 agggatacgg ttggctcttt cgcctgttgt gcgatgccga tgatgaggtg ctgattgcgg
    19681 cgccgagtta tccactgttc gattttctgg cgcagattga ggatgtgcgg ctcgtgtcgt
    19741 atccgctggt gtacgaccac ggttggcaga tcgattttcc ggggctgcgg gagcagatca
    19801 cgccgcgcac gagggcgatc gcagtggtgc acccgaataa tccgaccggg cactacacca
    19861 gagatgcgga gcggcgcgaa ctggagcaga tctgcgccga gcggaagctg gcactgattg
    19921 tggacgaggt ttttctggat ttcccgctaa cgtccgctgc aaaaactcgg caggatggcg
    19981 cacctcgaag ttttgccgcg gcggagcact cctgtctgac gtttgtgctg agcgggatca
    20041 gcaagattgc ggcgctgccg cagatgaagg tggcctggat tgtggcttgt gggccggagc
    20101 cggagcgggg cgaggctttg gagcggctgg aggtcatcgc cgatacgttt ttatcgatga
    20161 atggcccggt gcaatgcgca ctgccggcgt ggcttgcagg gagcgcagcg atccaggagc
    20221 aaattcgtgc acggactgcg acgaatttac agaatttaga tcacacactg ttgcgttatc
    20281 cagtcgtgac gcggctggag gtggaggcgg gctggtacgc cgtgctgcgg gtccccgcgc
    20341 tgggcaacga tgaggatctg gcggtgcgtc tcgtggaaga gtgcggggtg tatgtgcatc
    20401 cgggatcgtt cttcggattc gcgggtgagg ggtggctggt gttgagcctg ctgacgccgg
    20461 aggaagaatt tcggcgcggg gcagagcgaa tctgcgaaca tttcaaagcg cgcggggatt
    20521 gaacgatgca gatcccgatt aagcgaacgg tggagcggat tccgggcggg ttcatgctgg
    20581 tgccgctgct ggccggggcg gtagcgcaca cgtttgtgcc gaatgcggga ccgttcttcg
    20641 ggtcgttcac gggcgcgctg ttcacgggag ccctgccgat tctggccgtg ttctacgttt
    20701 gcgtgggcag ctcgattccg gtacgggcgc tggggcaggt ggtgaagcgc ggcggcgcgc
    20761 tgctcacgac aaagattgcg ctgggaattg cggcgggctt gatcctcgga cattttctcg
    20821 gcaacgatcc agtgcgcgcg gggtggttcg cgggcgtgtc gacgctggcg gtggtggcgg
    20881 cgttcaacga cacgaacggc ggcctctaca tggcgctgat gaaccagttc gggacggcgg
    20941 aggagacggc ggcctactcg gtgatggggc tggaatcggg accgtttctc acgatggtga
    21001 cgctgggtgt tgcggggctg gcgacgttcc cgtggcccac gctgctgggc gcggtgctgc
    21061 cgatgctggt gggactggtg gtggggaatg tcgatgagga gatgcgggag tttttcagcc
    21121 gggcgacgcc ggtgatgatt ccgttttttg cgtttgcgct gggcgcgacg ctgaacctga
    21181 agcaggtgtg ggccgcgggg ctggtgggga tcggactggg attgctcgtg ctgctggtgt
    21241 cgggcgcggc gctgatcggg gtggatcggt tgtgcggcgg aacgggcgta gccggggtgg
    21301 gcgctgcgac gacggcgggg aatgcagcgg cggtgccggc gctggtggcg gcggccaatc
    21361 ataaatatgc ggaagcggcg gctccggcga cggtgctggt ggcgtcgagc gtgatcgtga
    21421 cgacgatgct ggctccgctg gcgacggcat ggtgggcggc gcgagtccga aagaagggcg
    21481 atgcagatgc gcacaaacgc ggacgcgtgc tgattctggc cgatgatttg gcgggtgccg
    21541 cggatggtgc tgcggcttgc tgcgcgactg ggcgcgaggc tttgttattg ctcaacggcg
    21601 atggagcggt ggatgaggaa gtggtcgtgg tcgacgcgaa cacacgctgc cttggccctg
    21661 aggaagcatc gagagagatg cggcggctgg tgcagtgtta cgcgggtggg gggcgggtgt
    21721 tgttcaaaaa ggtggactcc acgctgcggg ggcatttagc gccggaactg gtggcggtgc
    21781 ttcaggcacg aagcgagatg ttgcgggaca ggcccgtggc ggtgtttgcg ccggcactgc
    21841 ccgcgcaggg acgcgtggtg ctggatggag agttgcgggt gcatggcgtt ccgctggaga
    21901 agaccgatgc gtggaaaaga gagcgtcgag gggcgcgttc cgatgtaatg gcgatgctgg
    21961 ccggcctcgg actgcgaacg acgcgattgc gtttggcaac ggtccggtcg ggagcatcgg
    22021 cgttgaagaa agccatggca tcggcagcgc aatacgcaga ggtcgtgatc tgcgatgcgg
    22081 agatggatgc agacctggcc gcgattgcgt tggcgtcgaa ggtgttggga gagcgggcgg
    22141 tgtgggtggg gtcgtcgggg ctggcgcggc acctgccggg gccgctgcga tcgagcgcgc
    22201 aaagggcggc ggcggttcca aagttcgcgc ccgaattcgc tttgggtccg actctatata
    22261 tggtgggaag tatgacggag atggcgcggg aacaggcaga gcggctcggc gcggtaccgg
    22321 gagtggagac gctgcggatt ccggcggcgg ttctccttgc ggaagcgtgg gcagaggaat
    22381 tgcggaggct ggaaattgcg ctcgagtcgc ggagggatgt gctggtggtg ccgcagtgcg
    22441 atgcgccttt gaactatgag cagggaacgg agttggtgca ggcgatgggg cgatttgttg
    22501 gcggatgcgc cgatcgtgtg ggaggactcg tcgccacggg cggcgagaca gcgcgcgcgg
    22561 tgctggatgc gtggggtgtg acgcgactgc gggtgcgagg cgaagtggaa gcgggcgtgg
    22621 cgttttcgac gacggaagga ggccggcgcg cgatgccggt ggtgacgaag gcgggtgggt
    22681 tcggcgatgc gcaaacgttg gtgcggggcg ccgaatttct gcgcgggctt aggcggcgcg
    22741 cgccggaagc ttcccaggcg gaggcgagtc gatgagcctt ccgttgattg cgatcacgat
    22801 gggcgatgcg gcgggtgtgg gaccggaggt caccatgaag acgctcgcgc atgcggaggt
    22861 gcatgcgatg tgccggcctc tggtgatcgg ggatgcggca cggctgcggg cagcggggaa
    22921 gattgtaggg agcggacttg aggtgcggag cgtcggcgaa gactcaattg cgacggcagc
    22981 attcgaccca ggacatgtga actgcgtaga tctgggttta gttccagctg atctggagtg
    23041 ggggaagttg tcggcgctcg ctggcgaggc ggcgtatcgg tacatcgaag ttgcggcgcg
    23101 gctggcgctc gccgggcgcg tggcggcgat ctgcacggct ccgttgaata aggaagcgct
    23161 gtggatggca gggcacaaat ttccgggaca cacggaattg ctggcgcatc tgaccggaac
    23221 gccggaggtg tcgatgatgc tgaccacgcc caagctgcgc gttcttcatg tgacgacaca
    23281 catcgggctg ctggatgcga tcgagaagat tgagccggga ctggtggagc ggacgattgc
    23341 gcgcgggcag gaagcgctgg cgcggatcgg gattgcaaag ccgcgcattg cggtgtgcgg
    23401 gatcaatccg cacgcgggcg agcatgggtt gttcgggcgc ggggaagagg agaccaagct
    23461 ggctccggcg atcgagaagt gccgggcgcg tgggtggaat gtggaggggc cgctgccggc
    23521 ggacacggtg ttctttcgcg cgcagcgtgg cgacttcgac ctggtgatcg cgatgtatca
    23581 cgatcaggga catgggccgg tcaaggtgct cggcctcgag tcgggagtga acatcacggt
    23641 ggggctgccg gtgattcgga cgtcggtcga tcacgggacg gcgttcgaca ttgcgggcaa
    23701 aggtgtggcg gacgagcgca gcatggtgga ggcgctgcga caggcggtgg aactggcgcc
    23761 gcagggctaa cgcaaatatt catacaccga acgaacgttt cgcgaatcaa aaatgcgtaa
    23821 tgtaggatcc tctcccaggg tgggagcgga atcgtcgctg gttcgggcca gctgcacggt
    23881 gagattttcc agggctctgc agggaatgga ggagccaggc atggaaggca ggacggcgat
    23941 tgtcaccggg gcttcgcaag gtattggagc tgcgattgtc gatgcgtttc tggggcgggg
    24001 ctacgacgtc gtcgccacgt cgcgccacgt ttcaggcgcg ggatatgcgg catctgcaca
    24061 tctggcgctg gtggacggag acattggcca ggcagagacg gcggagcagg tggcacgaat
    24121 agcgttcgac cggttcggat cgatcgatca tgtggtgaac aacgcgggga ttttcagggc
    24181 gaagcgtttc acggagtaca ccgccgatga tctgcgggcc ttcgttgcta caaatctgga
    24241 cggattcgtc ttcattacgc agctggcggt aaagcagatg ctgcagcagc gcacgggtgg
    24301 gagcgtgacc accataacgg catcgctggc cgacaatccc atcgccggcg tgccgggttc
    24361 gattccgatg atgaccaagg gcggtctgaa cgcgatcacg ctgagtctcg cgaacgaata
    24421 cgcaaaggac aagattcgct tcaacgcggt ggctccagga gttgtggaca cgccgatgca
    24481 tgcggggaag acggcggcgc gttcggcgat ggggacgttt tcccatccaa aggacattgc
    24541 cgatgctgtg atgtatctga cagaggctgg tcatgtgacc ggggaggtgc tgcacgtgga
    24601 tgatggtgcg catgtggggc agtggtgagg acggccggct ccggggcgga gcggaggctg
    24661 gcggagctgg gcattgaact gcccgcggct ccgacgccgt tcggcagcta cgtggagacc
    24721 ttgcagacag ggaatttgct gttcctgagc ggcatgctgc cggtggtcga tcacaagcca
    24781 aaatatttgg ggcgggtagg cgcggaactc gacgccgaga aggggcgcga cgccgcacgg
    24841 accgcgacgc tgggcgcgct ggcggcggcg aggaagcatc tcgggtcgct cgatcgggtg
    24901 acccgggtgg tgcggctggc cgtctttatg gcgacgtcgg gcgacttttt cgatcagccg
    24961 cgggtggcgg atggtgcgtc ggacctgctc cgcgatgtgt ttggggcgga gaagctgccg
    25021 gtgcggagcg tccttggcgt ggcgagtttg ccgctcggga tgccggtgga agtggaagtt
    25081 actttcgaag tgatggaata gctgcgcgtc cagcatgcgc ggttcaacga tgacgaacga
    25141 cccatttctg agaaacaaag aaggagagat cgatggcgac ggcagaaacc gcggttacaa
    25201 cgatgaaagc ggctcagatc ggaaagcccg gtgcaggttt tgagattgtc gagtgcagca
    25261 ttcccacgcc cggcgcgggc gaagtgcgga tcaaagtgca ggcgtgcggc gtttgccaca
    25321 gcgatgtgct cacggtggaa ggcgggtttc cggggattca gtatccgcgt gtgccgggac
    25381 atgaggtggc gggtgtgatt gacgcggtag gcgcgggtgt ctcggcctgg cgggtggggc
    25441 agagggttgg agtgggctgg cacggcggtc acgacaacac gtgtccggag tgccggcggg
    25501 gagacttcaa caactgccgg aacatgcaga ttgccgggat cagctacgac ggcgggtatc
    25561 aggaatatat gctggcgccg gtcgagggtt tggtgtcgat gcccgacagt ctcagcgatg
    25621 tggaggcagc accgctgttg tgcgctggag tgacgaccta taacgcgctg cggcacagcg
    25681 gagcgttgcc gggagatctg gtggcggtgc aggggatcgg cgggctgggg cacctggggg
    25741 ttcagttcgc gaacaagttc gggtataagg tcgcggcgat tgggcgcggt ccgggcaacg
    25801 ccgcgctggc gaaaaagctg ggcgcgcacg tctatatcga cagcaaggcg acgaacgcgg
    25861 cgcaggaact gcagaagctg ggcggggcaa aggtgattct ggccacggct ccgagttcga
    25921 agtcgatgtc ggaactgatc gacggactgg ggccgaacgg gaagttcctg gtggtggggg
    25981 cgtcgacgga tccgattgag gtgacgccgc tgcagctgat cttcggcgct aagtcgattc
    26041 agggctgggc gtcgggggtt cccatggatt cagaagacac gctgcgattt tcggagctga
    26101 cgggagtgcg cccgatgatc gagacgtatc cgctggagaa ggcagcagaa gcctatgcgc
    26161 ggatgatgag tgggaaggcc gagtttcgcg ttgtgctaac gatggcggcc ggctaacggc
    26221 cggtcggcca aaagcagctc aaaagcggtc agctaaagag cggttggcga cacgggcatt
    26281 gcagggtttg gagtcggagg cgaacgatgg ctttcgtgcg aggaccacgt ttcagcgggc
    26341 gcgcgctttt cttattgcta gcgctggcgg tgggtgcgag cgctccggcg caggaaagga
    26401 gagtttcgat ggcggcgcca attttctatc gaactgtgaa ggtggacggg ctgaacatct
    26461 tctaccggga ggcaggaccg aaggatgcgc cgacgattct gctgctgcat ggtctgcctt
    26521 cttcgtcccg catgtttcag ccgctgctga cgcggctggc ggatcgctat cacctggtgg
    26581 cgccggatta tccgggattt ggacacagcg actggcccga tccgaagcaa tttgcctaca
    26641 ccttcgatcg cattgcagtg gtgatggaag acttcacgca ggcgctcggc ctcgaccact
    26701 acgcgatgta catgcaggac tatggcggcc cggtgggatt ccgcatggcg ctgcggcatc
    26761 cggagcgggt ggaggcgctg atcgtgcagg acgcggtggc acacaacgag gggttgggcg
    26821 cgaactgggc gacgcggcgc gcgttctggg cagatcgggc gaagtatgaa gacgcgctgc
    26881 ggacgaatct gctttcgctg gcgacgacgc ggacgcggca tgtgggcgac gatcccaacc
    26941 tggaggcgta cgacccggat ctctggagcg atgagtacgc gtttctcagt gcgccgggcc
    27001 aggcgcagat ccagagcgat ttgttttacg actaccggac gaatgtcgag gcgtatccga
    27061 agtggcaggc gtggatgcag aagacgcagc cgcggctatt agtgatctgg ggcaaacacg
    27121 atttgtcgtt cgacctgggc gagcccgagc ggtatcgcaa agacgtgccg aaggccgacg
    27181 tgcacgtgct ggacgcgggg cattttgcgc tggacacgaa ggcggacgag attgccgggc
    27241 tggtgcggga atttctgggg cggaagtgag ggcggccaaa ccgaagcgtt cggcctgtat
    27301 catggagggt ggacgttccc cgttccgctg gacccttcct tttcatgcac tttttctttg
    27361 accgcaacgc ttgcatgggc acccgatggc tctgacaccg gccgcttccg gcgtggggcc
    27421 ggagattgtc ccgctggatt cgcaccgggg cgatggaccg cacctccacg cgccgagggc
    27481 aggcgagacg gcctcgctgc tggtggatta tcgcgagctg ttcaaggtgc gcgttaccgg
    27541 gctggtggtg ctcaccgggt gggccggttt ctatctgggc tcgatgcgct ccggtatcag
    27601 cagcctgcaa cccgggctgc tggagacgct gctcggcatc ggcgcggttt cggcagggtc
    27661 ggcggcgctg aatcaggcaa ttgagcggaa gctggatgcg aagatgcacc gcacggagaa
    27721 tcgcccgatg gcggcgggac gcatcggcct cgtgcacggc atcgggattg gcctgctggc
    27781 cattattgcg ggcacgcttt ggctgacacg cgagacgaat ttcatcaccg gtgggctggt
    27841 gctgctgacc gcctttctgt atgtggcggt gtacacgccg ctgaagcgct acaccacgct
    27901 cgcgacgttt gtgggtgcgt ttccaggagc gatgggtccg ttgctgggct ggacggcggc
    27961 gcgcggtgtg atcgagtggc agggcgtggc gctgttcgcg atccttttcg tctggcagtt
    28021 tccgcatttc atgtcgatct cgtggcttta tcgcgaagac tacggacggg ccgggattcg
    28081 catgctgccc gtggtgcagc cggatgggtg gtcgacggcg cttgaagccc tggtctacgc
    28141 ggtgctgatg atcccggcga gcctgctgcc gttctggctg catatggacg gcgtggctta
    28201 tgccgtggtg gcggtcgttc tgggcctggg atatctggct tacacgattc gcttcgcgcg
    28261 gattgtgcgc gcgcgcagcg agaccgaatc gagaatgtat gcgcgcgacc tgctcaaggt
    28321 cagcgtgatc tatctgcctc tgctgctgac ggcgctgatg ctgaatgcaa ggtagggaat
    28381 agccaatcgc cagcagggaa taagtctgat gccaaccgcc accaccagct cgaagctgaa
    28441 tcccaggccg aagacctccg ctgtggtgcc ggttgtggct ctggttggaa tcagcgtcgt
    28501 tgctgtgctg tttctgttgt ggctgatcta tgtgcatccg gcgtccgacg cggcggcgct
    28561 gcactacaca tttcttccca acctgaacgc gttgctcaat gggctggcgg ccattgcgct
    28621 gctggcgggg ttttatttca ttcgcacgca tcgcattgcc gcgcaccggg cgagcatgat
    28681 gacggcgttc gtcttcagca ccctgtttct ggtctcctat attgcaaacc atgcgctgca
    28741 cggcgacacg ctgtatccgg tccacaatcg cgtttactac gcggcgtatc tgcccatcct
    28801 gatcagccac attgtgctgt cgacggtggc gctgccgatt gtgctgatca ctttttattt
    28861 gtcgctctcg cggcgattcg ccgctcatcg caaactggca cgctacacct ttccgctgtg
    28921 gctctatgtc tcagtcaccg gggtcgtggt gcgggtgatg ctggtggcgg cgtttcgata
    28981 gacccatggc gcagggaaga cccatgcaat ccgagcagcc cgacgacggc acccgtgcgt
    29041 tgctgaaggc cgggggcggc atgctggccg cgggcattgt ggcggcggtg ctgacggcga
    29101 cggtgttcgg cgggatcagc cgccatggtc cgcactcgaa taccggatgg ctgttgctga
    29161 tggtggcgat gtcgtgcctg ccgttcggat tgatgctgtt cgcgctcggc gtagcgaagt
    29221 ggtttcggaa ccggaggaac agccagtagc cggtagaaag ttggaaggcc cacgctgagg
    29281 taaccggtgt ggttcgcaaa ggttgcagaa ggtctggtcc tggctcgccc cgccaggata
    29341 cgctcgtcaa cagaaggggt gcgactcgca tcccggaaaa actccccctc gatcgcgact
    29401 tcggtgtgcg atccgggttt aaccctgaac aaggccggcc ggctgaacag ccaggctgga
    29461 tgcaagcaag gagacaatgc acatgaaatt cgcaggccga ttcgtccacc tggcggtctt
    29521 cgccacttgt ttcgccctga ctcccttgct cgcacaatcg aatacacccg cgcagaatcc
    29581 gcagagcgcg caggaccaga gccagccggt gaccggcgtt tcgcatccgc cggccgacga
    29641 tacgattatg gccgatgagg atctgcccac tccggtgcca cggccgaaac cctcggcggc
    29701 gatcccgatc acgccggtgc agcctgccgc cactacggcg ccggcgctcc agcacagaaa
    29761 ttatgaagac gacattgtca cctcggctcc ggcgtccacg agctcggcat cgctctcgaa
    29821 gcacgcatgg aatcccgatg aagatatcgt gagcgtggtg gcgtcgaatc ccaacgaact
    29881 ggcgtcgggc acgaacgtgc gggtgcgcct gtccacggat ttgtcgaccg ccgagactct
    29941 gcgtgggcag tcgtttaagg cgatcgtcga tcgcgacgta tataaggacg gccgtatcat
    30001 tatcccggtc ggtgcggaga tgcgcgggcg ggtagttcag gtatcccagg gacaccacat
    30061 cggtcccaaa gcgacgctgc ggctgcggcc cgagtcgata ctgctgcccg acggcacgat
    30121 ctatcacatc tatgccgagg cggtgcattc gagcgcgccc ggcacgcgca ccgacaatga
    30181 gggcggcatc caggctgctc cgcactacaa aaaagacgcg gtggagtacg gcgccggagc
    30241 gggagccgga gccgcggcag gtgccctgat cgccggaccg gtgggcgcgg gcgttggatc
    30301 gctggtgggc gcgggggccg tgactgcgca catgatcgtc gggcaaccgg aagccgccaa
    30361 actgcccccg ggatctgtcc tgaccttcag cctgacggag ccgatggaat tgacgccgat
    30421 gcgggcgacg gcgaactaag aggcgtcagc cgtcgcttcg gatcggaatc gcctttcagc
    30481 accacgagtt cgtgcagctc gcgtcatcct gggcttctcc ctgaccggtg cgcagccgca
    30541 ttcctcgatg aactcgatcc gcttgcgtaa ctcgatggtt tggtcgcgca gaattgcgat
    30601 ctcacggcga agtgaatccg ccacgggtcc tgccggaccg cgcgtgcgcg tgcgcgccca
    30661 ctcgtccagt tcttgcggga tagccagcac ggcatggcgc gcggcaccat cgcgacggcg
    30721 cacgggcagt ccggcctcgc gctcccaccg ctgtacggtg cgcactccct tgccgagata
    30781 ctgactaatc tctttccatg aggtaaggat atttggcata gcgctccagc cctgggtaac
    30841 tggaaccatc gggtgtcctg acgcccggcg aatcctatct tccaattttg gttcttttgg
    30901 gtggaatttg cgagaaaaat cgtttattgt ctcgcgtcac gacatacccg gacagccggg
    30961 ctcttcaatt cactaccgat gtaaggatag cttccattga agcgaatcta tctttcccaa
    31021 accgggaaga taggtcgggc acgccagttt ttggcgtgct ttttctatcg aatatagcca
    31081 gatagcttga cgttccacaa cttatctcgc catactctcc acactacctc agcattagaa
    31141 acctgtattt cggctgcact gttcttcctt cctccccctc gcgcttaagt tgggaccgca
    31201 cgtcgccgca ggcggcaccg aacaggagtt ggaaatggct gaagttccgc gggaaaggcg
    31261 cgggggatcc ggagttccct cgctgaccgc aatctccctc cccttccctg gcctgtcctt
    31321 gcatcgtggc gtgcggttcc tgcccttacc ttctggtcac cgttgaaagg gaggaacgtt
    31381 atggtcagca actgtgccaa ccccgggtgc ggaaagcctt tgcactatct gcgtgacggc
    31441 cgaatttatg tttttgacac ggctgcgctc tcgaccagac ccggtcataa gcgtgaacgc
    31501 catctggaac actattggct ctgcggcgtc tgcgcgcaac ggctgacgct ggttcaggac
    31561 ggtcaggggt gggttcggat ccttcccaaa ccggcgttgc agggagacga ggacgactac
    31621 cttccctccc gtcatccgga aatggcctcc taagcaccct ctcccaccgc cagacgtccc
    31681 gcaggtttgc tgcggggcgt ctttgcgcct tgcactgggc gtcttgtttc ctgcaatctg
    31741 acggcaggaa gggaatcgtg caccgccttc acatcccggc gccgccgctg gcctcctttg
    31801 tccgttgttt ctggtactgg gaagggatgc cgcatccgca cgcgcgggag aggctgatgc
    31861 cgaacggcga ggccaccatt gttttcaatt tgcgcgacga cgagatgcgg ctctacgacg
    31921 ccgacgatct ggatcgctac acctcatgcg ggttggcggg gctgaccggg ccgcgtacgg
    31981 ggtgcttcgc catcgacacc gccgcgcagg atcgcgtcat cggcattgag tttcagccgg
    32041 gcggcacgtt tccgtttttt cgcgagccgg cgggcgagat cgcgaaccag tcggcgccgc
    32101 tcgactgcct gtggggcgcg gcggtggggg agattcggga gcagctgctg gccgcgcgtt
    32161 ccgtggatgc gatgttcgtg attctggcaa ctgcgctgct ggagcggttg gtgcggccgc
    32221 tggagctgca tccggcggtg ggctgggcgc gaggatggat ttgccgggcg ccccatgtgg
    32281 ccacggtggc cggcgtggtg gatcgcattg gactgagcca gcggcggttc attgagctgt
    32341 ttcgcgatca ggtggggctc cctccaaagg cgttctgccg ggtgcgtcgg tttcagcgcg
    32401 tgctcgagag cgtgcaccgc aaggctggcg tggactgggc gcaggtggcg ctggatgggg
    32461 gctactacga tcaggcccac ttcattcacg atttccaggg gtttgcaggg atgactccgg
    32521 cagcgtacct ggcgcgtgcg accgaacacc tgaatcatgt gccgatgggg tgagcgcagg
    32581 ggtcaaatat ttacaatcct gccggattca gaacgccgac aatggtttcc atcaaggaga
    32641 acccttatgt ccccagtgaa accgattccc gatggctacc acagcgtgca gccgtatctg
    32701 catgtacgcg gcgccgcgaa tgccattgat ttttacaagc aggtattcgg cgccgaggaa
    32761 gtgatgcgca tgccgcaacc ggacgggcgg atcgggcacg ccgagattcg tatcggcgat
    32821 tcgattgtga tgctggccga tgagtacccg gagaagggca tccacggtcc ggctcactat
    32881 ggcggcgcgc cgatgacgct gatgttctat ac
//
LOCUS       DASZYN010000545         9465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_730636, whole genome shotgun sequence.
ACCESSION   DASZYN010000545 DASZYN010000000
VERSION     DASZYN010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 9465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9465
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730636"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VHE33_14065"
     CDS             complement(<1..987)
                     /locus_tag="VHE33_14065"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT98623.1"
                     /translation="MNKLVIGNILFRPVRSVISVLAVAIEVIMILCIVAIMVGQVQGA
                     ANQTSGIGADIMVRPPNASNFSGLSDAAVPAKIAPQVLAKLPHVVAASPVIAKLNTSG
                     SVQTIFGIDYASYDALRPFVFIAGHPPAGPDDALVDDLYAKSHHVEVGQTLQLLGHDF
                     HISGIVEHGKGSRVFLPIHTLGVLNGTPDNASIFYVRTDKKENEQAVIDEIQHTPGMT
                     TYQVQTLAEWMSMMTPEHLPGFNIALNVVIAIAVIVGFLVIFQSMYTAVMERTREIGI
                     LKSLGASKLYVVNLVLREAVLVSIAGIVVGILATQGIKLLMDVKFPTLTFFLT"
     gene            1286..1762
                     /locus_tag="VHE33_14070"
     CDS             1286..1762
                     /locus_tag="VHE33_14070"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVT98624.1"
                     /translation="MGHFGEELRKERESRGIALETISEKTKVITRYLTALENDRFETL
                     PGGILSKGIVRGYARTVGLDEAMWVERFVAASQEHGHADGDWSEFVQNVGKTRPRSGH
                     EGRKLRRTGLWVLILALAGGGWAAWHYLGGGALADGIPQHAVSSTAAAAPASAVRP"
     gene            1830..2513
                     /locus_tag="VHE33_14075"
     CDS             1830..2513
                     /locus_tag="VHE33_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98625.1"
                     /translation="MTPRKLALLSGLTLMMPAMAWSLPKPHIVALGGVRRVPYSVAGD
                     PAGARPDETELTVRPLIVDGKVKEWTTGDVHDLTIRTFAVRRAVRLNDALPTDKTERW
                     VWQRGPWILVDRTVGKVTALRLPDFDPAVSDVVWFRDYAAYCGVNSGGKQLMAVVEQV
                     EGRKPLLAKKLGAWEAAEHSGPVCAPAVWQRDPLRVSFAVTGAATVSFDLVGTSAVLV
                     EEDEEPVAR"
     gene            complement(2578..3543)
                     /locus_tag="VHE33_14080"
     CDS             complement(2578..3543)
                     /locus_tag="VHE33_14080"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HVT98626.1"
                     /translation="MIFLAIIAVVGILGYTFIEKWNLLDSVYMVVLTLSTVGFEEVHP
                     LSPGGRLFTIFIIVGGVGSAIYAAGQIVEIIVAGEMTGYRKRRNMDKTIREMKGHYII
                     CGFGRVGHQVAQAFETWHVPFLVIDPKKESFDELESKGVPVLIGDATDDAVLTAAGIS
                     RAKGLIACSDSDVANVYITLSARQLNANLNIVARASLKDTEKKLTMAGANRVISPYFI
                     SGVRMAAMATQPVAVDFLDLVTHNGLVDFSLFQITIPTGSPLHGKTIAEADIRNTSGA
                     LVLAIRKSDGSFDLHPKETSRIDTNDVLVVLGTQEQFDSLQKMVQ"
     gene            3781..4377
                     /locus_tag="VHE33_14085"
     CDS             3781..4377
                     /locus_tag="VHE33_14085"
                     /inference="COORDINATES: protein motif:HMM:NF014693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HVT98627.1"
                     /translation="MESLEELLKQAEVAHGHLCAGQVLGVRMAMLGCRRLGIEEPRGA
                     DRKRLVTYVEIDRCATDAIGVVTGCRLGTRALKFRDWGKMAATFVDLGTGRAIRIVAL
                     ESSKQRARELYPEMENKNQQQMKAYREMPDAELFGEQWVQVEVGPKEMPGYKGERVTC
                     AVCGEGINYERFVERGGDRLCLACAHPEQRYYRPVNGD"
     gene            4521..5288
                     /locus_tag="VHE33_14090"
     CDS             4521..5288
                     /locus_tag="VHE33_14090"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HVT98628.1"
                     /translation="MLNLEGRVAAVFGLANKRSIAWGIATKLHEAGAKLAISYQNERM
                     KSEALGLIEELPGAEPFQCDVANDAEIDQVFAQIKEKYGKLDVLVHAIAYAPPEDLKN
                     DFLKTTREGFRIAHDVSVYSLIALSRGAAPLMTEGGSIITLTYYGAEKVVPHYKVMGV
                     AKASLEATVRYLAWDLGRQNVRVNAISAGPIKTLAARGIGALGDMLKYHEDRSPLHRN
                     TEQTEVGKTAVYLASDLASGVTGEVIYVDSGYNIVGF"
     gene            5515..6993
                     /locus_tag="VHE33_14095"
     CDS             5515..6993
                     /locus_tag="VHE33_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98629.1"
                     /translation="MQLGDLLVQARLASAEDIAKALERQSSEGGRLGENLVAMGVLTE
                     SALDRFLHHIPTEPADIAATGIERVELMGLMMKLIYSARLQTIRQITDAIKLPYRMVA
                     ELVQMAVDRKLLQSMGVRGADSSGGTSYAFTDGGSRFAVEALGQVGYVGPAPVPLDQF
                     NDQVTHQKLTNEVITFERIRKSIGSLTFDDALIEQCGPGLNSGRAMLLYGPPGNGKTS
                     IARCLASVFSDIIYLPYAIAVEGQIIRLFDPAIHVAVDSAGVSEDPGFLADRSDILDH
                     RWVACRRPFLVAGGELTLEMLDLLYNPIGHYYEAPIHIKALGGCFIIDDFGRQLVSPT
                     ALLNRWIIPLENRVDYLKLHTGKSFSIPFEELVIFSTNIEPEDLMDPAFLRRLPYKIE
                     VGAPSIDSFRRIFETECASHGIALSSETFDSIVNKIVNEKGLELAAYQPRFIVDQVIA
                     TCRFMEKAPQMEPRYIEYALDNLRVHRNSPPQTAHAPQLQQA"
     regulatory      7077..7150
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            7273..7650
                     /gene="crcB"
                     /locus_tag="VHE33_14100"
     CDS             7273..7650
                     /gene="crcB"
                     /locus_tag="VHE33_14100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HVT98630.1"
                     /translation="MQKYLLIALGGGLGSMARYWVGVTVTDRLGARFPYGTLIVNLTA
                     CLIIGFSLTFLGRRAGFSGGWIYLLPVGFVGAYSTFSTYEWETLSLLRAGGFPMAALY
                     AVGSLILGLAAVWCGMLAAEVMR"
     gene            7647..8390
                     /locus_tag="VHE33_14105"
     CDS             7647..8390
                     /locus_tag="VHE33_14105"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HVT98631.1"
                     /translation="MNAREGSSMLGTGKALKVSITVSEGARYHGASAYASILDFLFYR
                     GVSGATVLKGIAGFGADHHLHAASLVEVSDKLPVRIEFVETREKVNELLGKLEEMAGT
                     GLIEVQETMVAKAPMPSKQKRVTPDHLKMEGKARLMRIYVGESDKWQNKPLHEALVHA
                     MRANDIAGVTVVRGVLGYGAHRRANKEKPAHLSKDASILLTVVDTQEKLQSFLPVLEQ
                     MVQEGLVVMSDVDIVKYSWRPPELEASEA"
     gene            8404..8757
                     /locus_tag="VHE33_14110"
     CDS             8404..8757
                     /locus_tag="VHE33_14110"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HVT98632.1"
                     /translation="MKEQFEAQMLRIYFGEADRWMGKPLHEAIVAKCQELGLQSAIVY
                     RGIEGYGSSTRIHRPSPWKLSRDAPIMVSVIDTEEQIGKLVPHLDAMVGEGLIVTSKV
                     QAIRYTRGEEQAKGA"
     gene            complement(8767..>9465)
                     /locus_tag="VHE33_14115"
     CDS             complement(8767..>9465)
                     /locus_tag="VHE33_14115"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVT98633.1"
                     /translation="NTQATIVGVVGHVLHWGLDNDAGTPLHAQLYQSMGQLPGSELVS
                     TTGFFYNIAVRADHPDTVFPDIQSSLREMNASQVAWRPLTMNQIIAASLVARRFSMVL
                     LAAFAALALLLASVGLYGVISYLVGQRTQEMAVRMALGADRSDVLRWVLRRGATLAVI
                     GVGAGTVTALLVTRALAGISVARSSIIYGVHPWDPITLIGVVAVLMFVALLASYVPAR
                     RAAAVDPMQALRSE"
BASE COUNT         1898 a         2383 c         3304 g         1880 t
ORIGIN      
        1 ggtgaggaag aaggtcaggg tcgggaactt cacgtccatg aggagcttga tgccctgagt
       61 ggccaggatg ccgacaacga tgccggcgat ggagacgagc acggcttctc gcaggacgag
      121 gttcacaacg tagagcttcg aagcgccgag cgatttgagg atgccgatct cgcgggtgcg
      181 ctccataacc gccgtgtaca tggactggaa gatgaccaga aagccgacga tcacggcaat
      241 cgcgatcaca acgttgaggg cgatgttaaa gccggggagg tgctcggggg tcatcatgga
      301 catccactcg gcgagggtct ggacctggta ggtagtcatg cccggggtgt gctggatctc
      361 gtcgataacc gcctgctcgt tttccttttt gtcggtgcgg acgtagaaga tggacgcgtt
      421 atcgggggtg ccgttcagta cgccgagagt atgaatggga aggaagacgc ggcttccttt
      481 gccgtgctcg acgattccgg agatatggaa gtcgtgcccg agcagttgca gagtctggcc
      541 gacctcgacg tgatggctct tcgcgtagag gtcgtcgacg agggcgtcat ccgggcccgc
      601 cgggggatgg cccgcaatga acacgaaggg gcggagcgcg tcgtagcttg cgtagtcgat
      661 gccaaagata gtctggacgg atccgctggt gttgagcttg gcgatgaccg gcgaggcggc
      721 gacgacatga ggcagcttcg ccaggacctg tggagcaatt ttggctggca ccgccgcgtc
      781 gctaaggccg gagaagttgc tggcgttggg cggccggacc atgatatcgg cgccaatgcc
      841 gctggtctgg ttggctgccc cctggacctg gccgaccatg atggccacga tgcagaggat
      901 catgatgacc tcgatcgcga cggcaagcac gctgatgacc gaacggacgg ggcgaaagag
      961 gatattgccg ataactaatt tgttcatcgg attgttggat gaagcgaaca gaactccggg
     1021 tggaaggaac agggtaacaa agcgatgagg agaggttaaa aagaggcggg aacggagacg
     1081 gacgagggtg attggaggga ggcggccctg cggttatgac ccaaaaggga acagagggtg
     1141 gcccgagcgt gggatttcct taggaggtta ccttggtacc attatctccg caatataaac
     1201 gggtaatcag agtttcgatt ttctttgacc tatcgtaggg tcggccttat cttttactgg
     1261 caatccccag gaagggaatc ctgcagtggg gcactttggc gaggaattgc ggaaggaacg
     1321 ggagtcccga gggatcgcgc tggagacgat cagcgagaaa actaaggtaa tcacccgtta
     1381 cctcaccgca ctcgaaaatg accgattcga gacgctgcct ggcggcattc tgagcaaggg
     1441 catcgttcgc ggatatgcgc gcaccgtggg gctggacgaa gctatgtggg tcgagcggtt
     1501 cgttgccgcc agtcaggaac acgggcacgc cgacggcgac tggtcggaat ttgtgcaaaa
     1561 cgtggggaag acgcggccca ggagtggtca cgagggcaga aaattgcgca gaaccggtct
     1621 ttgggtgttg attctggcgc tggcaggcgg tgggtgggcg gcctggcatt atctgggcgg
     1681 gggtgccctg gccgatggga ttccgcaaca tgccgtcagc tcaacggctg cagcggcgcc
     1741 ggcgagcgct gttcgtccgt aaccgcttct cttctaaaaa gtacttctgt gaccttttgc
     1801 gggcggtagt gtcctcttag cctgcaagca tgactcctcg aaaactggct ctgctgtccg
     1861 gtctgacatt gatgatgccg gcgatggcgt ggtctctccc aaagccgcac attgtggcgt
     1921 tgggcggcgt acggagggtt ccctattccg tcgcgggcga cccggccggc gcgcggccgg
     1981 atgagacgga gctgacggtg cgcccgctga tcgtggatgg caaggtgaag gagtggacga
     2041 ccggcgatgt gcacgacctg acgatccgca cctttgcggt tcggcgtgcc gtacggctga
     2101 atgacgcgct gccgacggac aaaacagagc ggtgggtctg gcagcgcgga ccgtggatcc
     2161 tggtggatcg gacggttgga aaggtgacgg ccctgcggct gcccgatttc gatccggcag
     2221 tttcggatgt ggtgtggttt cgggactatg cggcgtattg cggcgtgaat tctggtggga
     2281 agcagctgat ggcggtggtg gagcaggtgg agggtcggaa gccattgctg gcgaaaaagt
     2341 taggggcctg ggaggcggcc gaacactcag gtccagtgtg tgcgccagcg gtgtggcagc
     2401 gggatccgtt gcgagtgagc tttgcggtaa cgggcgcggc gacggtgagt ttcgatttag
     2461 tggggacgtc ggcggtgctg gtggaagagg acgaggagcc agtagccagg taggagatgg
     2521 agcggggagg acgcgagagg taagcccagg ttccggcgcc gagtggagat cgcgttttta
     2581 ttggaccatt ttttgcagcg aatcgaattg ctcctgagtt ccaagaacaa ccaggacatc
     2641 attcgtgtcg attcttgagg tttccttcgg gtgcaaatcg aaggagccat cggatttgcg
     2701 aatggccagc accaacgcac ctgaggtgtt tctgatgtcc gcttccgcaa tggtctttcc
     2761 atggagcggc gaaccggtgg gaatggtgat ttgaaacagg ctgaaatcga ccagcccgtt
     2821 gtgcgtcact aagtccagaa agtcgacggc cacgggttgc gttgccatgg ccgccatgcg
     2881 aacaccggag atgaaatagg gcgaaatgac ccggtttgcg ccagccatgg tgagcttctt
     2941 ctctgtatct ttgaggctcg ctctggccac gatgttgaga ttcgcattga gctggcgggc
     3001 ggagagagtg atgtagacgt tggctacatc ggagtcggag caggcgataa gacctttggc
     3061 gcgactgatg cccgcggcgg tcagaacagc gtcgtcggtg gcatcgccga tgagaaccgg
     3121 aacgcccttg ctttcgagtt catcgaaaga ttcttttttg gggtcgatca cgaggaaggg
     3181 aacgtgccag gtctcaaaag cctgggccac ctggtgtcct actctgccaa agccgcagat
     3241 gatgtaatgg cccttcatct ccctgatggt cttgtccatg tttctcctct tgcgataacc
     3301 ggtcatctcg cccgcaacga tgatctctac aatctgtcca gccgcataga tcgcgctgcc
     3361 gacgccgcca acaataatga atatggtgaa gagcctcccc cctggcgaaa gaggatggac
     3421 ttcctcgaac ccaacggtgg agagggtgag aacgaccatg tacacggaat ccaggagatt
     3481 ccatttctcg atgaaggtgt agcccaggat tccaacgaca gcaatgatcg cgagaaagat
     3541 cattgcggga aggagtcttc gaaaagggtg gctcatcttg ggcccgtgcc tggctttctt
     3601 tgccgtccgt gtgatggcga tcccggcaac gctccagcgg tcggatgcat gcgatcagag
     3661 taatgcactg cggaggtgtt caacccgaga gtgtgttgtc gtcggttttg cggacattac
     3721 aggagtacct cggcggttgt tgcgctaccc cgtatttcgc tcgcgcctta tgatcggtga
     3781 atggaatcgc ttgaggaatt gctgaaacag gccgaggtgg cgcatgggca cttgtgcgcc
     3841 gggcaggtgc tgggcgtgcg gatggcgatg ctggggtgca ggcggctggg aatcgaggag
     3901 ccgcgcgggg cggatcgcaa acggctggtg acgtatgtcg agatcgatcg atgtgcgacg
     3961 gacgcgatcg gggtggtgac cgggtgccgg ctggggacgc gggcgctgaa gtttcgcgac
     4021 tgggggaaga tggctgcgac gttcgtcgac ctcgggacgg gcagagcgat tcgcattgtg
     4081 gcgctggagt cgtcgaagca gcgggcgcgg gagctgtatc cggagatgga gaacaagaac
     4141 cagcagcaga tgaaggcgta tcgcgagatg ccggatgcgg agttgtttgg agagcagtgg
     4201 gtgcaggttg aagttgggcc gaaggagatg ccggggtaca aaggcgagcg ggtaacgtgc
     4261 gccgtatgcg gggagggaat caactacgaa cgctttgttg agcgcggcgg ggaccggtta
     4321 tgtctggcgt gcgcgcatcc ggagcagcgc tattatcggc cggtgaatgg ggactagcca
     4381 tccaggcgat cgggccttgc ggcccaaagg cggtcgttgt ggtttgaggg ttccggccct
     4441 gagtctggtt gcacatttac aatggcaggg ccggtagtga tttggttgcg ccggacgctt
     4501 ctcctggagc acaacgaacc atgctgaatc tggaaggccg tgtagcggct gtttttggac
     4561 tggcgaataa gcgcagcatc gcctggggca ttgccaccaa gctgcatgag gcgggggcga
     4621 agctggcgat cagctatcaa aacgagcgaa tgaagtccga ggcgttgggc ctgatcgagg
     4681 agttgccggg ggcggagccg ttccagtgtg atgtggcgaa cgatgcggag atcgatcagg
     4741 tttttgcgca gattaaggaa aagtacggga agctggacgt gctggtgcac gccatcgcgt
     4801 atgctccgcc ggaggacttg aagaacgatt ttctgaaaac cacgcgggag ggatttcgga
     4861 tcgcgcatga cgtgagcgtc tactcgctga tcgcgctgag ccgcggcgcg gcaccgctga
     4921 tgacagaagg cgggtcgatc atcacgctga catattacgg ggccgaaaag gtggtgccgc
     4981 attacaaggt gatgggtgtg gcgaaagcgt cgctggaggc gacggtgcgg tatctggcct
     5041 gggatctggg caggcagaac gttcgggtga atgcgatttc agcgggcccg atcaaaacgc
     5101 tggcggcgcg gggaattggg gcgctgggcg acatgctgaa gtatcacgag gatcgctcgc
     5161 cgctgcaccg gaacacggag cagacggaag ttgggaagac ggcggtgtat ctggcgtcgg
     5221 acctggcgag cggcgtgacc ggcgaagtga tctacgtgga cagcggctac aacattgtag
     5281 ggttctgagg gtgcagggcg cagaccacag gtcacagaga cctcgaagct ttctcccaat
     5341 cctatcgagc tggcgcggaa gaggtcccgc gaacctcaca ggtcatggct tcttccgtgg
     5401 tattggggct ccgttcgctc cgtgctatca atagggacgc gcggcgctcc cttaacgacg
     5461 tgagcgcgaa cttgttccca ggtcacttcc tcggaggatg tcatcgccac gcgattgcag
     5521 ttaggggatc tactcgtcca ggccaggctg gctagcgctg aggacatcgc caaagcgctc
     5581 gaacggcaat cgagcgaagg cggccggctg ggtgagaacc ttgtcgccat gggagtgctc
     5641 acggagagcg cactcgatcg atttctgcat cacattccca ccgagcccgc cgacattgcc
     5701 gccaccggaa ttgagcgcgt tgagctgatg ggcctgatga tgaaactcat ctattccgcc
     5761 cgtttgcaga ccatccgtca gatcaccgac gccatcaagc ttccttatcg tatggtggcc
     5821 gaactggtgc agatggcggt cgatcgcaag ctgctgcaat cgatgggcgt gcgcggcgcc
     5881 gacagctccg gcggcaccag ctatgccttt accgacgggg gatcgcgctt tgcggtcgaa
     5941 gccctcgggc aagtgggcta tgtgggcccc gctcctgtgc ctctggatca gttcaatgac
     6001 caggtgaccc accagaagct gacgaacgaa gtcataacct tcgaacgcat ccgcaaatcg
     6061 attgggtccc tcacatttga cgatgccctg atcgagcagt gcggaccggg actgaactcc
     6121 ggccgtgcca tgttgctcta cgggccgccg ggaaacggaa agacctccat cgctcgctgc
     6181 ctggccagcg tcttcagcga catcatttat ctgccctatg ccatcgctgt tgagggccag
     6241 atcatccgtc tgttcgaccc cgcgattcac gtcgccgtcg attccgccgg cgtcagcgaa
     6301 gatcccggat ttctggcaga ccgcagcgat attctcgacc accgctgggt tgcatgccgc
     6361 aggccctttc tggttgccgg cggcgagctt accctcgaga tgctggacct gctctacaac
     6421 ccgatcgggc attattacga ggccccgatc catatcaagg cgctgggtgg ctgtttcatc
     6481 atcgacgact ttggccggca gctggtcagc cctacagccc tgctcaatcg ctggatcatc
     6541 ccgctggaaa accgcgtcga ctacctcaag ctccataccg gcaagagctt cagcattccc
     6601 tttgaagaac ttgtaatctt ctccaccaat atcgagccgg aagatctgat ggaccccgcc
     6661 tttttgcgtc gcctgccgta caaaatcgag gtgggtgccc ccagtatcga ctccttccgt
     6721 cgcatcttcg agacggagtg cgccagccac ggcatcgcgc tttccagtga aaccttcgac
     6781 tcgatcgtta acaagatcgt gaacgaaaag ggcctcgaac tggccgccta tcagcccaga
     6841 ttcatcgtcg atcaggtaat cgccacgtgt cgtttcatgg agaaggctcc gcagatggag
     6901 ccgcgctaca tcgaatacgc gctcgacaat ttacgggtgc accgcaattc cccgccgcag
     6961 acggcccatg cgccgcagct gcaacaggcg tgaaacaccc ggagaaacag gctggcgttt
     7021 atcccctctc aggagacatt gcttgacatt gctgtgggtc tgatttgtag tggaagtaat
     7081 caagcggcga aggagttcgc ctttaaccgc tgtcctttgt agccggacag atgatgactc
     7141 ctgacaggga atcctgtcgt ggagtcgttt cccggcgcga tccctgacaa attgagattt
     7201 cgttgcgcgg cagacgtgtg tctgttccgg caccgggttg gcctgttgtc tctggccgtt
     7261 aaggaggggg gattgcaaaa gtatctgctg attgcgctgg gcggcggact aggttcgatg
     7321 gcccgctact gggtgggcgt aacggtgacg gatcggctgg gggcgcgctt tccgtatggg
     7381 acgctgatcg ttaatttgac ggcttgcctg atcattggat tttcgctgac gtttctgggg
     7441 aggcgcgccg gatttagcgg aggctggatt tacctattac cggtagggtt cgtgggggcg
     7501 tacagtactt tctcgaccta tgagtgggag actctttcgc tactgcgagc gggcggcttt
     7561 ccgatggcgg ccctttatgc ggtgggaagt ttgattctgg ggctggccgc ggtttggtgc
     7621 gggatgctgg cggcggaggt gatgcgatga acgctcgtga aggaagcagc atgctgggga
     7681 caggcaaggc gctgaaggtt tcgatcacgg tgagcgaagg ggcgcggtat cacggcgcct
     7741 cggcgtatgc gtcaattctg gattttttgt tctatcgcgg agtgagcgga gcgaccgtcc
     7801 tgaagggaat tgcggggttc ggggcggatc atcatctgca cgcagcgagc ctggtggaag
     7861 tgtcggacaa gctgccggtg cggattgagt ttgtcgagac gcgggagaaa gtgaacgaac
     7921 tgctgggcaa gctggaggag atggcgggca cgggcctgat cgaggtgcag gagaccatgg
     7981 tggcgaaagc tcccatgccg tcgaagcaaa aacgcgtgac gccggatcat ttgaagatgg
     8041 aaggcaaagc gcggttgatg cggatctatg tgggcgagag cgacaagtgg cagaacaagc
     8101 cgctgcacga ggcgctggtg cacgcgatgc gggccaatga tattgccggg gtgacggtgg
     8161 ttcggggcgt gctgggatat ggagcgcacc ggcgcgcgaa taaagaaaaa cctgcgcatt
     8221 tgtcgaaaga cgcatccatc ctgctgacgg tggtggacac gcaggagaag ctgcagtcgt
     8281 ttttgccggt gctggagcaa atggtgcagg aggggctggt ggtgatgtcg gatgtggaca
     8341 ttgtgaagta ctcgtggcgg ccgcctgagt tggaggcgag cgaggcatga gggaggcgtg
     8401 gcgatgaagg agcaatttga agctcagatg ctgcgcattt actttggcga ggcggatcgc
     8461 tggatgggga agcctctgca cgaggcgatt gtggcgaagt gccaggagtt gggtctgcag
     8521 agtgcgattg tctaccgggg gatcgaaggt tatgggagca gcacgcggat ccatcgtccg
     8581 agcccgtgga agctgtcacg ggacgcgccg atcatggtca gcgtgattga tacagaagaa
     8641 cagatcggga agctggtgcc gcatctcgac gcgatggtgg gcgaggggct gattgtgaca
     8701 tcgaaggtac aggcgatccg atacacgcgc ggggaggaac aggcgaaggg cgcgtgatgc
     8761 tgcgatctat tcggagcgca gggcctgcat gggatcaacg gcggcggcgc gccgcgctgg
     8821 gacgtagctc gcgagcaatg cgacaaacat cagaacggcg acgactccga tgagcgtgat
     8881 ggggtcccac gggtggaccc cgtagatgat cgaggatcgg gctacggaga tgccggccaa
     8941 ggcgcgcgtg acgagcaggg ccgttacggt gcccgcgccg actccgatga cggcgagggt
     9001 ggcgccgcgg cggagaaccc agcggagcac atcgctgcga tcggcgccga gagccatgcg
     9061 gacagccatt tcctgggtgc gctggccgac gaggtagctg atgactccat agaggccgac
     9121 gctggcgaga agaagggcga gagcggcaaa cgctgcgaga aggaccatgg aaaagcgtcg
     9181 ggcgacgagc gaggcggcga tgatctggtt catggtgagg ggacgccacg cgacctggga
     9241 agcattcatc tcgcgcagcg aggactggat gtcggggaaa acggtgtcgg gatggtcggc
     9301 gcgaacggca atgttgtaga agaagccggt ggtggagact agctccgagc cggggagctg
     9361 gcccatggac tggtacaact gggcgtggag tggagtgcct gcgtcgttgt cgagtcccca
     9421 atggagcacg tgaccaacga cgccgacgat ggtggcctgg gtatt
//
LOCUS       DASZYN010000546        18843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_345771, whole genome shotgun sequence.
ACCESSION   DASZYN010000546 DASZYN010000000
VERSION     DASZYN010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 18843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18843
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345771"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VHE33_14120"
     CDS             complement(<1..495)
                     /locus_tag="VHE33_14120"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HVT98634.1"
                     /translation="MEKHDETDGRNVSPRLTRRQTLPALAGLVGAGIMGSGRAPGEEV
                     PAAVDAGARVYNVRSYGARGDGRTLDTIAVQRAIDACASDGGGIVLIPAGTFLLGSVE
                     VKSNVILRIAAGGKILGSANGHDYHAVDAIPLSGDSTLEDGNWALLYAVNASNVTIEG
                     PGTID"
     gene            complement(955..4527)
                     /locus_tag="VHE33_14125"
     CDS             complement(955..4527)
                     /locus_tag="VHE33_14125"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HVT98635.1"
                     /translation="MSRARFESRFCCTIVLLAMPALAAAQNNLFTTVTPSTVAAAPNR
                     VATGDFNGDGIMDMAVTVPSQDVVDVFIGTGDGNFGSPVSYAAGTNPWTLVAGDFNHD
                     GHLDLAVTNALTNGHVSILFGKGDGTFDTNGGTYLAGVFPYAIGSGDFDGDGNLDLAV
                     INFGQTSTPGSVTILLGKPGGTFQFASTYTFGAYPATIAVGDFTGDGKPDLAVGFAGP
                     VGSSLSQVQIFTNGGGGSFQASALLAAPVPDGIASITAGDFDGDGKLDLAAVSAFART
                     VTVFHGRGDGNFDAVPASPSIGAGPQWIAAGDVNGDGKVDLVSANLDGTVSVLLGNGD
                     DTFTSNATYQVGNQPVMLALADLNGDGKLDVTVVNSGDDNVQVLLGKSDGTFFTGSFQ
                     VGANPGGIAAGDFNGDGFADLAVAKGDATVAIFLGDGRGAFKALNPFPACQGEAGYGL
                     GKIVAADVNGDNKVDLAVVCNSTTTGSSFGTVFQGNGDGTFTTALGAPGNSQGMIAAD
                     INGDGVPEFITASGGGIIAFNLSPAFVDIVSTAFAPGGVAAGDFNGDGKTDLLAIGSG
                     ATPGVILLGDGLGNFQQDSVLGWPGGLTGDFNGDGLTDFLNYQPSDLVQVILNQGNGT
                     FLSGTEIQGNPIGRPQALADFNGDGALDVLTAFHSGAPLNVFLGHGDGTFDQQPAVSL
                     SQLGMATVAVGDFDANGSPDVAALDSSTGTVSILLNRNSFQSTVTTLTGPATKAVVGQ
                     SFTLSATVSSKQGTPTGNVTFKQAGVPQTTGSLNNGKAQATLSAPSTVGTFGFTALYT
                     GDGTFGGSVSQRLLLSVSVASSTTVVSSSNRNSKLGQSVTLTATVSPQFSGVPTGTVA
                     FYADGQPIGSGGMSGGQASVSISTLAEGSHIIEADYSGDTNFTTSLGRVTQKVGTASS
                     AVTLASSLNPAVYGQPVTLTATVTDSAGTAPTGMVVFSEAGTIYGSVTLSGGMAQLTL
                     PQLLVGKHAIIAQYSGDSSDAPSKATLTETIQGASSSTAITSSLNPSNYGQAVVFTAT
                     VTSTAGSPDGTVTFKNGTQGLGTVTLSGGQAQLSVTTIDAGAHTIKAVYNGSNTYTTS
                     QNSLQQVVVAAPTTVTLTGSPNPASLGQSVTFTATVNCATATPVGTVSFKDGKTALGT
                     ATVVNGQAQISTSLLTKGGHTLTATYSGSADFSKSSGAVSQAVQ"
     gene            5400..7193
                     /locus_tag="VHE33_14130"
     CDS             5400..7193
                     /locus_tag="VHE33_14130"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98636.1"
                     /translation="MGDFANSTGDPLFDGSLRRAAVIQLAQSPYLNILPDQKLGEVLQ
                     DLGRSPDDKLTPALALEVCQRGRASALIIGSIQRTGGAYLLALEASRCVDGSSLVHET
                     VTVAHQNAVLSSLGSMIDDLRRSLGESRDSLQKFDVPIEQATTSSLEALKAYQLGLEL
                     RAHSKNLEARPAFKTAVALDPNFAIAYAQLGSSYSNEGETQEGRKCFQKAFELRAHAT
                     EPERLYIVGRYFDIVTNEREKGSETYKLWTELYPDQWLAYNALANDANLLGRYETVVD
                     ASEHAVHLDPSHNFGYINLLSGLIALNRLDEASSICQRLIGQGRDDTFIHLSLFSIAY
                     LRHDEQALSREREWAQRHPDGTSMQYVEAETDAAMGKVRESTDLFEHVAKRNVASGDT
                     EAAANTLATAAEINSEVGRTALAQKESEEALRLRSNQIVLGIGALIASRARNGERAHE
                     LLDALDHDYPLSTFNIGLYSPMARTTLAVARRVSTSEITSLMEPALPYEFGQVADMLP
                     IYVRADSYLQARSAEEARREFQKVLDHHAVDVVTTLYPLSELGVARCYALLGRKADSR
                     RAYEDFFALWKDADRALPVLVMARREYQELK"
     gene            7479..7886
                     /locus_tag="VHE33_14135"
     CDS             7479..7886
                     /locus_tag="VHE33_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98637.1"
                     /translation="MALNSVVLSSQKLDLNDALYNAQRGKEQARDEAANPLVQKGQKL
                     IPRVTRVFNVDRPMYVYLQAYDWAEVANTAGSTAASAITPLFAYVSSCRDEKAAMETP
                     PIAVTLQPATRLGVVPLSFEVGLGKSAPGQYQC"
     gene            complement(8277..9224)
                     /locus_tag="VHE33_14140"
     CDS             complement(8277..9224)
                     /locus_tag="VHE33_14140"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HVT98638.1"
                     /translation="MSVQITRRAFAGMCAAFSVFPAQQTPSPQHPGDRVYDIFGVPFR
                     AGSLYPGTENDAAAYRQAGLVDQMGAAGICVADCGDIPIPSFLPHHNIPPIRNWPAPR
                     IVWDCVSEQIHPSLKQPGHVPLLIGCDCSIVVGTVQALRNAGSKSVHVLYFDGDFDDA
                     APEPQVCNSAASFATWLLTSPSPFWSGPQLDHSQLTIVGWSVGSKARGQQSASISLAE
                     IRGAGVNRLAGRILESIPSSADILIHLDIDVLAKQEMPAAYFQHRDGLTLAECAELLG
                     PIARDSRVRLIEISEYATLRDLSGIYMQALIALLTRALR"
     gene            9473..10522
                     /locus_tag="VHE33_14145"
     CDS             9473..10522
                     /locus_tag="VHE33_14145"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HVT98639.1"
                     /translation="MKAGSNRRLQLVAGTTPSRSISDHQTRVAVLALPGSALSDLAGP
                     WETFLLAGRLAQKKLGLTRPCYEVALLSLEGSSVSTLSGLEISGACPFDAYQGVPDTL
                     VVVGEPDSMQERKDYSALFEWLRRVTRESRRVCSVCTGAFCLAQAGLLDGCRVTTHWQ
                     YARALAQRHPAVQVDPEPLFLRDGKFYTSAGCTAAMDLSLALVEEDVGFEIATEIARD
                     MIMFLRRPGRHAQISPLLKLQMSERKPMRELQTWMLENLNLPLTVEDLAEQVHMSPRN
                     FARSFVAAVGTTPARFLETLRLEVARRRLEETDAGMDQIASDCGFGSAEVMRRSFARH
                     LGVPPSAYRRQSDLA"
     gene            complement(10743..13949)
                     /locus_tag="VHE33_14150"
     CDS             complement(10743..13949)
                     /locus_tag="VHE33_14150"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT98640.1"
                     /translation="MSSMFRFVGQACFAATLAVLLGPAFPTLHALEQTENRAVRTTAS
                     VPTVALIGRVRDRSGRPIAGAAISLSPLIGGRKMSAASNPDGSFQFTAVSAGEYRLEV
                     GAPGYKTFVVARLPLVSGDVANANPVLDPGKQVEIMAAAQDSVTSRMGTALAGKSVSD
                     LPENQRNFVNLVQVSAGANEGSTNASASGSRPGAQHESSAVSIGGEAESANYSQIDGM
                     DNNERINSRIAVHPSVEGIASVQIFANAYPASMGKASGGFINVVTKSGSDTMHGSLYE
                     YFRNDVLDAFPYQFGAGNPRPELRQNQFGGSFGGPLVRGKTHYYADYEGFRLVQGRAP
                     VALTVPTAYEHANPGDFSDVGGPVLTHLDPAGLAYFRLYPLPNVPGSPDQFVSAPSGL
                     NFSHIFDARIDQRFSRRDQIFGRVSYNRTLVYIPGQFPSVQQAGMTIEPGGSLTSFAG
                     NMHDTGVNAMFDWSREVRPNLAFDLRGGYVFWHESDTGLNPHVAVNQGFGQPGINLAS
                     TSNGLAPIDVFRASPLGTDGYWRPLDQGDHTIQVSGALSFNRGAHAVTAGAALVHRQW
                     FDIGSGDGLGLWKVEDLPHLLLGQFVGVDREVDLVEPHLRVREWSAFLEDTWKATPQL
                     TLDLGLRYDLFTPPTEAQNRLANFDFTSGHIVIAGRDGVSATAGVQTDRTNLGPRFGF
                     SWNLGHSSVLRGGYGLVFRVPHDGFVYKTQPFAYTFGVCSSATCPGGYTSLAAGLPEP
                     VAPDPQNPTGDLWGTRSFHERMLQLHQFNLGIEKQFGANTARVFYVGALGRHRTRYFP
                     DFNAPPPNVSSDPDSLRPWHAVVPNLTAIDYSDTEGSSSYHALQAALAHELRRGLTAH
                     LNYTWAHGLDNDSGQGFGTVPALSSKIDYGNSNVDVRHRAVATLFYDVPLGRNAVGVR
                     RLVLAGWQANFAAAWSAGLPYTVLNANDVSNTNPGASAADRPNQVGSAHLAHPAVAEY
                     FNIHAFAAQVPGTLGSERSNQLFGPPTRHLDGSLFKNFGLGSEKTLQLRTEVFNLTNT
                     ASFASPAAILGGANFGHLTQMTAGYSPREIQLALRFEF"
     gene            14298..15461
                     /locus_tag="VHE33_14155"
     CDS             14298..15461
                     /locus_tag="VHE33_14155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HVT98641.1"
                     /translation="MLQTREETAIRYPGFGKLLLGWAVLAGVACIRHVLLDNVPHAHL
                     AREFADWLTCYLPWVLLTPAVFRLERKFPLNLAQWRRNAALLLAAGLVLSYAGGTIAD
                     VLFSLERIVLEPSAAAAPWWKMSGSEFLFQFALYCVALLAACLIRKLIEMQESERRAA
                     RLTLEKSELEASLRRSELEMIRMRLNPHFLFNSLQNLSTLARQNPDAASRMLARLGDV
                     LRAALRTSAPAQTRLSSEIELTQAYVEVEQIRFDGRLSVLFEVEPGMDNALVPGFLFQ
                     PLVENAITHGLRDGQTSGAIWVRAVRHGEDLVLTVRDNGSGPPADRLADLEMGIGLGS
                     TCERLERMFGDRHRVSVNRLPEGGTEVRITLPLEWAAGPAPSQVHEYVSSPHR"
     gene            15433..16191
                     /locus_tag="VHE33_14160"
     CDS             15433..16191
                     /locus_tag="VHE33_14160"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HVT98642.1"
                     /translation="MNTFRVLIVDDEPLIRAGIRDGLARMAEIEIAAECGSGAEALRQ
                     LSSLSIDLVLLDVQLQDCTGLDVVERIGPERMPAVIFVTAYDEYAVKAFELNAVDYLL
                     KPFDDARLRRSVERACRRIAEHRQSDLAGQLQALLQARLRKWPERIVVRDGDRFDFVP
                     VDSIDWVESANNYVQLHCGPGRHLLSESLTNLESRLNPARFVRIHRCRIVNLTRLEAI
                     HPLPGGTFELELRNGTRLTTGRQYRNQIQALLRS"
     gene            complement(16225..16740)
                     /locus_tag="VHE33_14165"
     CDS             complement(16225..16740)
                     /locus_tag="VHE33_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98643.1"
                     /translation="MESSLFPTAALAAYLQSYTALLPPGFLKRITAQSLQSLAASPVR
                     MPMPDIESVIHLVPYLHPDQRTAPVRKLKDVLAAVVVLDPKHWDSYNVKPLTFVHSPQ
                     SPFYPEMKQAISANPDYIISIQKSDGSWGLTWSWADRDPAAWKVAEKEWLGVVMLENL
                     RTLQAFHRIAP"
     gene            complement(17152..18171)
                     /locus_tag="VHE33_14170"
     CDS             complement(17152..18171)
                     /locus_tag="VHE33_14170"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HVT98644.1"
                     /translation="MLWRDGIYDLIREVMPMQGSLGVERMCLLARVSRAGFYRFLKAS
                     VPREEDTEVRSAIQRVALEHRRRYGYRRVTAELKHRGMHVNHKRVARIMREDNLLALQ
                     PGKLVSCADSYDALEVYLNLPRRMRPNSVDQLWVADITYIRLQGEFVYLAIVLDGYSR
                     KVLGWKLDRALTSRLAVEALERAIDLRQPLPGVVHHSDRGVQYVSPEYVAILKRHGMI
                     QSMSRPANPYDNASCESFIKTLKREEIYANKYRDLEDLHSHIEEFIDGYYNLKRLHSA
                     LGYQTPEEYEAQTHGKTKAQLYAPACSFSRLVSRKLKGHGRQNSEANASRSPHGYRAS
                     NTWRQ"
     gene            complement(18141..18494)
                     /locus_tag="VHE33_14175"
     CDS             complement(18141..18494)
                     /locus_tag="VHE33_14175"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVT98645.1"
                     /translation="MRGRELQLAQRRVGKYPLSFRHMAVERMKDCPSVSALAKELGID
                     RSNLYEWQRQLEDGSGTSARVTSPVRELRKQVRELQRVLAEKTLEVDFFKGALQKVEA
                     RRQSSTCSGGTASTT"
     gene            complement(18617..>18843)
                     /locus_tag="VHE33_14180"
     CDS             complement(18617..>18843)
                     /locus_tag="VHE33_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98646.1"
                     /translation="CRKLSSHGEKQARNLDQTLLACGGLCAVATSVFGRIPAFMEGRE
                     NSSELVICIDLSQSVAARKRPRSRLLTEGL"
BASE COUNT         3786 a         5423 c         5756 g         3878 t
ORIGIN      
        1 atcgatcgtt ccgggcccct cgatggtcac gttggacgca ttcacggcat agaggagcgc
       61 ccagttaccg tcttcaagcg tggagtcgcc gctgagcggg atggcgtcga ccgcgtggta
      121 gtcgtggccg ttcgcgctgc cgagtatttt tccgccggca gcgatacgga ggatcacatt
      181 gctcttcacc tcgaccgatc cgagaaggaa cgtccccgca gggatcagaa cgatgccgcc
      241 accatcggaa gcgcatgcgt cgattgccct ttgcacggca atcgtgtcga gcgtacggcc
      301 gtcgccccgc gcaccatagc tgcggacgtt gtaaacgcgt gcacctgcat cgactgcggc
      361 tggtacctcc tcgcccggag cgcgcccgct gcccatgatg cccgcgccga cgagtcccgc
      421 gagtgccggc agcgtctgcc ggcgcgtgag tcgcggggac acattgcgcc cgtctgtttc
      481 gtcgtgtttc tccatcaact ctccctcggt acctgcgcag acgcactacc atgcgaaaca
      541 cgtctgccat cttgagaaaa acacggggca ggccatgtcg caaattgggg cactcctcgt
      601 tgtaccacta tatgagattg ctatctcacg ggtgcgtctc agcacctcag tcgctacgcg
      661 atccccaaac ctgtaaaagc gcggtggaaa tatgctcgaa cacatggttt gcgtcacagc
      721 aggcttatag atttcggacc tgaacgagcg atcaactcgc agagtagctg aagagcgtct
      781 gattgccact aatgccggct ggccgcgtaa cggccgacgc gctctcttga tagcccgcgt
      841 catgggcatt cggcaaaagg gcgcgtcggc gacaatacgg cgaggcggat cgcggaacac
      901 tggcccgttt cctgatactt attcctaatc ccgcgccgtc tgccgaaggt ttgtctattg
      961 gaccgcttgc gagactgctc cgctgctttt ggagaagtca gcggaaccgg aatacgttgc
     1021 cgttagtgtg tggccccctt tggtcaggag cgacgtgcta atctgcgcct gtccgttgac
     1081 gaccgtggcg gtaccgagcg cagtttttcc atctttaaag ctgacggtac caacgggggt
     1141 tgcggtcgcg cagttcaccg ttgccgtgaa cgtcaccgac tgaccgaggg acgccgggtt
     1201 gggtgaacca gtcagcgtca ccgtggtcgg ggcggcaacc acgacctgct gcaggctgtt
     1261 ctggcttgtg gtgtatgtat tgcttccgtt ataaaccgct ttgatcgtat gcgcgccggc
     1321 atcgatggtg gtcaccgaga gctgcgcctg ccctccgctc aacgtcacgg taccgagccc
     1381 ctgggtaccg tttttaaagg tgaccgtacc atctggactt cccgcggtcg atgtgacggt
     1441 tgcggtgaaa acgacggcct gaccgtagtt ggaaggattg aggctgctgg tgatcgccgt
     1501 ggagctcgac gctccctgga tggtttctgt gagggtagcc ttcgatgggg cgtcggagct
     1561 atcgccgctg tactgcgcga tgatggcgtg ctttcccacc agtaactgcg gaagcgtcag
     1621 ttgtgccatg cccccactga gcgtgacact tccgtagatc gtgcctgctt ccgagaaaac
     1681 aaccatcccc gtcggcgcgg tgccggcgga atcggtcacg gtcgcagtca aggttacggg
     1741 ctggccgtag acagcggggt tgagagaaga cgccagagtc accgcgctcg aagcggttcc
     1801 gaccttttgt gtgacgcggc cgaggctggt ggtgaagttt gtatctccgg aatagtctgc
     1861 ttcgatgatg tggctgccct cagcgagcgt gctgatgctc acggaagcct gcccaccgct
     1921 catgcctcct gaaccaatcg gttgcccatc ggcataaaag gcgactgtac cggtcgggac
     1981 acccgaaaac tgtgggctga ccgtagcagt gagggtgacg ctctggccga gcttggaatt
     2041 cctgttgctc gaggaaacca ccgtggtgct cgaagcgacg ctcacgctta gcagtagcct
     2101 ttgcgaaaca ctgccgccga aagtgccatc gccggtgtag agcgcggtga aaccaaacgt
     2161 gccgacggtg gatggtgctg acagcgtcgc ctgagccttg ccgttgttga gggagccggt
     2221 cgtttgcgga acgccggcct gtttgaatgt gacattgccg gtgggtgtgc cttgcttgct
     2281 gctcacggtg gcggatagcg tgaacgattg cccaacgaca gctttcgtcg cgggtccagt
     2341 tagtgtggtg acggtggact ggaagctatt tctgttaagc agaatggaaa ccgtaccagt
     2401 actggaatcc agggctgcca catccggcga tccatttgcg tcgaaatcgc caacggccac
     2461 ggtagccatt ccgagctgac tcagggaaac cgctggctgt tggtcaaatg tcccatctcc
     2521 atgaccgagg aaaacgttca acggtgcccc actgtgaaac gccgtgagca catcgagcgc
     2581 gccatcgcca ttgaagtccg caagcgcctg aggacgtcca atcggattgc cctgaatttc
     2641 cgtgcccgag aggaaggtac catttccctg attcaggatg acctggacga gatcgctagg
     2701 ctgataattc aggaaatctg tgagtccatc accgttgaag tccccggtta atccccctgg
     2761 ccagccgagg actgaatctt gttggaagtt tcccaagccg tcgcctaaga gaataacccc
     2821 cggcgtggcc cccgaaccga tggcaagcag atctgttttg ccgtcaccat tgaaatcgcc
     2881 cgccgctaca cctcccggag cgaatgccgt gctgacgatg tcgacaaatg cggggctaag
     2941 gttaaatgcg atgatacctc cgccactggc agttatgaac tctggaactc catcaccatt
     3001 gatgtcggca gcaatcattc cctgagagtt gccgggggcg cccaatgccg tcgtgaaggt
     3061 cccgtcaccg ttgccctgga agacagtgcc gaaagaagag cctgttgtcg tagagttgca
     3121 cacgaccgcg agatctactt tgttgtcccc attcacatcg gccgctacga tcttccccag
     3181 cccatatcct gcctcaccct ggcaagcggg aaagggattc agagccttga acgcgccccg
     3241 accgtcgccc agaaaaatgg cgacagtggc gtcccctttt gcgaccgcca gatcggcgaa
     3301 tccgtcccca ttgaagtctc ctgcggcaat accacccgga ttggcgccta cctgaaagga
     3361 tcctgtgaag aaagtgccat cgctttttcc cagcagaacc tgcacgttgt catcgccgct
     3421 gttcaccact gtgacatcca gcttgccatc gccgttaagg tccgcgagcg cgagcatcac
     3481 gggttggttt ccgacctgat aagttgcatt gctggtaaac gtgtcgtcgc cattgccgag
     3541 gagaacagaa accgtgccat cgagattggc cgagacgaga tctaccttgc catcgccatt
     3601 tacatccccc gcagctatcc actggggtcc tgcaccgatc gacggggagg ccggtaccgc
     3661 atcgaaattt ccgtcaccgc ggccgtggaa tactgtcacc gtgcgtgcaa aggcgctgac
     3721 cgcagcgaga tccaatttgc cgtccccgtc aaaatctccc gcagtaatgg acgcaatgcc
     3781 gtcaggcacc ggagcggcga gcaaggcact ggcctggaag cttccgccgc ccccgttcgt
     3841 aaatatctgg acttggctta acgaacttcc aaccggtccc gcaaaaccca cggccaggtc
     3901 tggttttcca tcgccagtga agtctcccac cgcaatcgtt gccggatacg caccgaaagt
     3961 gtaggtactc gcgaactgaa aagtaccgcc tggtttgccc agcaggatag tcactgaccc
     4021 gggcgtagag gtctgaccga agttaattac agcaagatcg agatttccgt cgccgtcaaa
     4081 atcgccggat ccgattgcat aggggaacac tcctgccaga taggtgccgc cgttggtatc
     4141 gaaggttcca tctcctttgc cgaaaagaat agaaacatgg ccgtttgtca atgcgttcgt
     4201 taccgccaga tccaggtgac catcatggtt gaagtcgccc gcgacgaggg tccagggatt
     4261 cgtaccggcg gcatagctta caggggagcc gaagttgccg tctcccgttc cgatgaaaac
     4321 atccaccacg tcctgagagg ggacggtaac agccatgtcc atgatcccgt cgccgttgaa
     4381 gtcgccggta gcgacccggt taggcgcagc cgcaaccgtg cttggcgtta cagtggtgaa
     4441 caggttgttt tgcgcggcgg ccagcgccgg cattgccaaa agaacgatcg tgcagcagaa
     4501 gcgggattcg aagcgggcgc gagacatagg tgctctcctg ttgagcggta gtgccgagat
     4561 cattgccagc catgtcgcga tccgcgccca ggtgggagtg ttgcccatcc agaggagcag
     4621 cggatgcgag ccgagaatca gaagcggccc cggagcgggt ttcgcagggc acgcttctgc
     4681 tttcgcagcc aggggactac tcgaatgtga gggtggatcg gtgcgggcgc aaattaagcg
     4741 tgcgccactt gatgactaac ccgtaaacca aatgcgcaca atgcgttacg ctcatggatt
     4801 aaccggattg ccgaagtggc gggagcccgt ggtagacttc cgtccactgt tggcaggctt
     4861 tttcccccat gagagattcc gtcgattccg cagcacccgg cattgaatac cgcttcgggc
     4921 tgtttgtgct ggatccggca agcggggctc tgacgcggaa cggaacaaga gttaagctcc
     4981 aggatcaacc tctcagactc ctgactttgc tcgttcagag agccggtcag gtcgtctcgc
     5041 gcgaggagat tcaacgaaac ctctggcccg ggaacatctt tatcgaattc gacaaaagtc
     5101 tgggggtagc cgtcgccaaa gttcgggagg ctctggcaga cgacgccggc aaccctcgat
     5161 tcatcgaaac gatcccacgc cgcggatacc gctttatcgc accagtcagg gcagagggca
     5221 ccgtggtcct cgagcacgcg gttgaggccc aggcagccgt ctccttacag agagcttcag
     5281 gttcataccg gggcaagctg atttgggtcg ccgtagcgtt gggtggagcg cttcttgcag
     5341 ctgcctggta ctggcggaat caaacgcatg gtcgcattcc ggcgcgcgct tccgtggttg
     5401 tgggcgattt cgccaacagc accggcgatc ctctgtttga cgggtcgctc cgccgggcgg
     5461 cggtcattca gctcgcgcaa tccccttatc tcaacatctt gcctgatcaa aagctgggag
     5521 aggttctcca ggacctgggg cgttcgccgg atgacaagct gacacctgct ttggcgctgg
     5581 aggtctgcca gcgcggaagg gcatccgcac tgatcatcgg atccatccag cgaacgggag
     5641 gcgcttatct gttggcattg gaagccagcc gctgtgtcga cggcagttct ttagtgcacg
     5701 aaaccgtgac ggttgcgcat cagaacgcgg ttctgtccag cttaggttcc atgattgacg
     5761 atctccgccg cagtctgggt gagtcccgcg actctctcca gaagttcgat gtgcccattg
     5821 aacaggcaac gaccagttct ttagaagccc tgaaagccta tcagttggga ttggaactgc
     5881 gcgcccactc gaagaatctt gaagccaggc ccgccttcaa aaccgccgtt gcattggatc
     5941 ccaattttgc cattgcctac gcgcagctgg gatcctcgta ctccaacgaa ggagagacac
     6001 aggagggcag gaaatgcttc cagaaagcat ttgaacttcg tgctcacgcc acggagccgg
     6061 agcggcttta tatcgtgggg cgctatttcg acattgttac gaatgaacgg gaaaagggat
     6121 ccgaaaccta caagctgtgg accgaactat atcccgatca atggctcgct tacaacgcgc
     6181 tcgcgaacga tgccaatctg ttagggcggt acgagaccgt tgttgacgcc tctgaacatg
     6241 ccgtccatct tgatcccagt cacaacttcg gatacatcaa cctgctttcc gggctcattg
     6301 cattgaatcg cctcgatgaa gcaagttcga tatgccagcg gctcatcggg caagggcgtg
     6361 atgatacctt cattcatctc agcctcttct ctattgcgta cctccggcac gacgaacagg
     6421 cgctgagccg tgaacgcgag tgggcccagc ggcatccgga cggcacctcg atgcagtatg
     6481 tcgaggccga aacagacgct gcgatgggca aggtcagaga aagcaccgac cttttcgagc
     6541 atgtagcgaa acgaaatgtc gccagcggcg atacggaagc agcggcgaat acattagcaa
     6601 ctgccgccga gattaattca gaggtggggc gaacagccct ggcccagaag gagtctgagg
     6661 aggctttaag acttcggagc aatcaaatcg tgctcggcat tggagctttg atcgcaagtc
     6721 gggcacgaaa cggagagaga gcccacgagc ttctcgacgc actcgaccac gattatccac
     6781 tgtcaacatt caacattggt ctttactctc cgatggccag gacgacgctg gctgtcgcgc
     6841 gccgcgtttc aacctccgaa attacgagtc tgatggagcc ggcgcttccg tacgagtttg
     6901 gacaggttgc agatatgctg ccaatctatg tgcgagcaga ttcctatctg caggctcgtt
     6961 cggcagaaga agccaggcga gagttccaga aggttcttga tcaccatgcg gtggatgtgg
     7021 tgactacgct ctatcccctt tctgagttgg gcgttgcgcg atgctacgca ttgttgggca
     7081 gaaaagccga cagccggcgt gcctacgagg atttcttcgc actttggaag gacgctgacc
     7141 gggctctgcc tgttctcgtg atggcgcgga gggaatatca ggagttgaag tagccggcat
     7201 cagtgctttt cgaagcgggt ctagagcgga aggagcaaag gtcccgataa gggagcgctg
     7261 gccgatccgc tgatgagcaa tgtccggggt tacgtgaaca ttaaactgag ccacgcgacc
     7321 gcggcccagc tcgcccatcg cccggtggag tacgacgcag gattcacccg gtttcctgaa
     7381 aaatatacga ttaagtttct tgcccgcgac gatgataagt actttccaga cccagttcac
     7441 aattccggat tccggacctg aacaaggtag ccaggaaagt ggcactgaac tccgtagtgc
     7501 tgagcagcca gaagttagac ctgaacgatg cgctctacaa tgcgcagcgc ggtaaggaac
     7561 aggcaaggga cgaagccgcg aatccgctgg tgcaaaaagg gcagaaactg atcccgaggg
     7621 tcacgcgcgt cttcaatgtc gaccgtccga tgtacgttta tctgcaggcc tatgactggg
     7681 cggaagtggc gaacacggcg ggatcgacag ccgcctcggc gataacgccc ctgtttgcgt
     7741 atgtgagctc ttgccgcgac gagaaggcgg cgatggagac gccgccgatc gcggtaacat
     7801 tgcagccggc gacacgccta ggggtcgtgc cgctcagctt tgaagttgga ctgggaaaga
     7861 gtgcgcctgg gcagtaccaa tgctaggtta gcgtgctcga cccagccggg caccgagcgg
     7921 cgttctggca ggggcggtaa tcctggtgca gtaggcgtcg cttgcggccg gtccgactct
     7981 ccattgttaa ggtttcccag tggggttaga ccgttcgcat ttcggcagac ttcctcgatt
     8041 gataccgaga agtcggccaa tgtttacgaa ttcagtccac ttggccctaa aacgggctat
     8101 ccggagaagt cgatggtcaa cagaactggg gactccaatg atggacgatt gcccaaatgg
     8161 tttattcgtc cgggatgggc cgcatgcggc aacctgtcga tgcagtttac tactggaaag
     8221 tcggcaggta tccattgaag ggcacgatcc gaactctcga cggtaatgaa ctatgatcac
     8281 cgaagcgctc gggtcaaaag tgcaatgaga gcctgcatgt aaatgccact aagatcccgg
     8341 agcgtggcgt attcactgat ctcgatcaac cgaacccggg agtccctggc gattggacca
     8401 agaagttccg cgcattccgc aagcgtcaat ccatcccggt gttggaaata ggcggcgggc
     8461 atctcctgtt ttgcgagcac atcgatgtca aggtgaatga ggatgtcggc agaggacggg
     8521 atggattcca ggatgcgtcc tgccaaacga ttcacgccgg ctccccggat ttccgcgagc
     8581 gaaatcgaag cagactgttg gccgcgcgcc tttgacccaa cgctccaacc cacgatcgtg
     8641 agttgggaat ggtccaactg aggaccagac cagaatggag atggactggt gagtaaccag
     8701 gttgcgaagc tggctgcgct gttgcaaacc tgcggctcag gtgccgcatc atcgaagtcg
     8761 ccgtcgaaat acaacacatg tacgcttttt gatccagcgt ttctgagggc ctgcaccgtc
     8821 ccaacgacga tgctgcagtc acatccgatc aacagaggta cgtgccctgg ctgtttcaga
     8881 cttggatgaa tttgctcgct gacgcaatcc catacaatcc gcggcgccgg ccaatttcgt
     8941 attggcggaa tgttgtggtg cggaagaaaa cttggaattg ggatgtcccc gcaatccgcg
     9001 acacaaattc cggctgctcc catctgatca acgagtccgg cttgccggta ggcggcagca
     9061 tcgttttccg tgccggggta cagagatccc gcacggaatg ggacaccaaa tatgtcgtag
     9121 actcgatcgc cagggtgttg gggcgatggg gtttgttgcg ccgggaatac agagaacgct
     9181 gcacacattc cggcgaaggc ccgtcgggtg atttgtacgc tcatgtcgca cggatcccaa
     9241 tcatgttctg atgtttcgcc ggatgaaatt cgagtacaag gccattctcc ccacgatttc
     9301 ggccaatttt tgcgtgccgc tcagcctgca gattcagggg ctccacttcg cattggaact
     9361 atctgccttt cggcgtgaaa gactgtgttt ttgtccttga agcccagcca atgatgagca
     9421 cagaattgtt ctgtactgcc gcaggctcag cgggcgacgc gaaagaaatc gaatgaaagc
     9481 aggctcgaac agacggctgc aactcgtcgc cgggacgact ccttccagaa gcatttcgga
     9541 tcatcagacc agagtggcgg ttctggcctt acctgggtcc gctctttcag atttggccgg
     9601 accctgggag acattcctgc tcgcagggcg gctcgcccag aagaagttgg ggctcactcg
     9661 tccctgctat gaggtggctc tgctttcgct tgaagggagt tccgtttcga ctctctccgg
     9721 attagagatc tcaggcgctt gcccattcga cgcatatcaa ggtgtaccgg acaccctcgt
     9781 cgtcgtgggc gagcctgatt cgatgcagga gcgaaaggac tactccgcgc tcttcgagtg
     9841 gctgcgccgc gtgactagag aatcccgacg cgtatgttca gtctgtactg gcgcattttg
     9901 tctggctcaa gcgggcctgc tcgatggttg ccgcgttacg acgcattggc agtacgcgag
     9961 ggctttggcg cagagacatc ctgcggtcca ggtggatccg gaaccgctct ttctgcgcga
    10021 tggaaagttc tacacgtcgg ccggttgtac ggcggcgatg gacctgtccc tggcccttgt
    10081 cgaggaagac gtcggctttg agattgcaac ggagatcgct cgcgacatga tcatgttcct
    10141 gcgccgacct ggccgtcatg cgcagatcag ccccctgctg aaactgcaga tgtctgagcg
    10201 caaacccatg cgcgaattgc agacctggat gctcgaaaat ctgaatctcc cgctgaccgt
    10261 agaggatctc gccgagcagg tgcatatgag cccacgcaac ttcgcacgtt cctttgttgc
    10321 tgctgtcgga acgacgcccg ctcgatttct tgagacgttg cgtctggaag ttgctcgccg
    10381 tcgactcgag gaaaccgatg caggcatgga tcagatcgca tccgactgcg gattcgggtc
    10441 cgcggaggtt atgcgccgtt cgttcgctcg tcacctcgga gtcccgccca gtgcttaccg
    10501 ccgccagagc gatctcgcat gatgcatcgt gaccaagcca cgcccaacgt tggtggctcc
    10561 cggtgcgatc gagttgagcg atgatgtgag gtgcattctc tgaagaattc atctggatct
    10621 gcagatcgga tcggaagtga tgtgagcgcc cctcgccagg ctgtgtcgaa gcgggcacgg
    10681 tctgatcgtt gacttgcttt ctggactctg cggcgatcaa ttcattctcg attcgccggt
    10741 tctcagaact cgaagcggag agcgagctgg atctcgcgcg ggctgtatcc ggcggtcatc
    10801 tgggtgaggt gcccgaaatt cgcacctccc aggatggcgg ctggtgacgc aaagctcgcc
    10861 gtgttggtga ggttgaaaac ctcggtgcgc aactgcaggg tcttctcgct gccgaggccg
    10921 aagtttttga acagcgatcc gtcgagatgg cgggtgggtg ggccaaagag ctggttgctg
    10981 cgctcgcttc ccagggtgcc cggcacctgc gctgcgaagg catggatgtt gaaatactca
    11041 gcaacggcgg ggtgggcgag atgggccgag ccgacctggt tggggcgatc ggccgcgctc
    11101 gcgccagggt tggtattgct gacatcgttc gcgttcagca cggtgtaggg cagaccggca
    11161 gaccacgcgg cggcgaagtt cgcctgccag ccggccagga caaggcggcg gacgccaaca
    11221 gcattcctgc ccaggggcac atcgtaaaag agagtggcca ccgcgcggtg gcgcacgtcc
    11281 acattgctgt tgccgtaatc gattttcgaa gagagagcgg ggacggttcc gaagccctgg
    11341 ccgctgtcgt tgtcgaggcc gtgcgcccag gtgtagttca ggtgcgcggt gagcccgcgg
    11401 cgcaattcgt gggccaaggc cgcctgcaat gcgtgatagg acgaagaccc ctctgtatcg
    11461 ctgtagtcga tcgcggtgag gttgggaaca accgcgtgcc agggtcgcag actgtcggga
    11521 tcggacgata cgttgggggg cggagcgttg aaatcgggaa agtaccgggt acgatgacgc
    11581 cccagcgcgc ccacatagaa gacgcgcgcc gtatttgcgc cgaactgttt ttcaatgccc
    11641 agattgaact ggtggagctg cagcatccgt tcatgaaacg accgcgtgcc ccataaatcg
    11701 ccggtggggt tctggggatc gggggcaacc ggttccggca aacccgcggc gagcgaagtg
    11761 tacccgcccg gacaggtggc cgaagagcaa acgccgaatg tgtacgcgaa gggctgggtt
    11821 ttgtagacaa acccgtcgtg gggaacgcga aacacgagtc cataaccgcc gcgcaacacg
    11881 ctgctgtggc cgaggttcca gctgaagccg aagcgagggc cgaggttcgt gcgatcggtt
    11941 tgcacgcccg ccgtggcgga aactccgtca cgcccggcta tgacgatgtg tccggaggta
    12001 aagtcgaaat tcgcgagacg attttgggcc tccgtgggag gcgtgaaaag gtcgtagcgc
    12061 aaaccgagat cgagcgtgag ttgcggtgtg gctttccacg tgtcctcgag gaacgccgac
    12121 cattcccgga cgcgcaggtg cggttcaacc agatcgactt cacggtcgac gcccacaaac
    12181 tggcccagca gcaggtgagg cagatcttcg accttccata agccgaggcc gtcgccgctg
    12241 ccgatgtcga accactgacg atgaaccagc gcagcgccgg cggtgacggc atgagcgccg
    12301 cgattgaagg agagcgcgcc gctcacctgg atggtgtggt cgccctgatc cagaggacgc
    12361 cagtagccat cggtgcccag cggagaggca cggaagacat cgatgggcgc gaggccgttg
    12421 ctggtagagg cgaggttaat gccgggttgc ccaaacccct ggttgacggc gacatgagga
    12481 ttgaggccgg tgtccgactc gtgccagaag acgtagccgc cgcgcagatc gaacgcgagg
    12541 ttggggcgga cctcccggct ccagtcgaac atcgcgttca cgccggtgtc gtgcatattt
    12601 ccggcaaagc tggtgaggga gccgccgggc tcgatcgtca tgccggcttg ctggaccgac
    12661 ggaaactggc cagggatgta aacgagtgtc cggttatagc tgactcgccc gaagatctga
    12721 tcacgccttg aaaaacgctg atcgatgcgc gcgtcgaaga tgtgagaaaa attcagtcca
    12781 ctgggagcac tgacaaactg atcgggagat ccgggaacgt tgggcagcgg gtagaggcga
    12841 aagtaagcca gtcccgccgg atcgagatgg gtgagaacag ggccaccgac gtcgctgaaa
    12901 tcgcctgggt ttgcatgctc atatgcggtg ggaacggtga gagccacagg cgcgcgtccc
    12961 tgcacgaggc ggaacccctc atagtcagcg tagtagtgag tcttgccgcg gaccaggggt
    13021 ccgccaaagc tgccgccaaa ctgattctgt cgaagctcag gcctgggatt gcctgcgccg
    13081 aactggtagg gaaaggcatc cagaacatca ttgcgaaaat actcgtagag cgatccgtgc
    13141 atcgtgtcac tccccgattt tgtgacgacg ttgatgaatc cgccgctggc tttgcccatg
    13201 gaagccggat aggcgttggc aaagatctgg acgctcgcaa tgccctccac ggaagggtga
    13261 accgcgattc tggagttgat gcgctcgttg ttgtccatcc cgtcgatctg actgtaattg
    13321 gcggactccg cctcgccgcc gatcgacacc gccgatgact cgtgctgcgc tcctggacga
    13381 ctgccgctgg ccgaagcatt ggtgctgccc tcgttcgccc cggctgaaac ctgaacgaga
    13441 ttgacgaagt tgcgctgatt ctccggcagg tcgctgacag atttaccggc gagtgcggtg
    13501 cccatgcgcg acgtcacgct gtcctgtgcg gcggccatga tctcgacttg ctttccagga
    13561 tcgagaacag ggttggcatt ggcaacgtct ccactgacga gcgggaggcg cgcgacaacg
    13621 aaggttttgt atcccggcgc gccgacctca agcctgtact cgccggcgga taccgcggtg
    13681 aactggaacg agccgtccgg attgctggcc gcgctcatct tccggccgcc gatcagcggg
    13741 ctcagggata tggccgcacc ggcaatcggc cttcccgacc ggtcacgcac gcggccgatc
    13801 agagcgactg tggggactga cgccgtggtg cggacggcgc gattctcagt ctgctcgagg
    13861 gcgtgcaagg ttgggaatgc cggtcccagg agaacggcga gtgtcgctgc gaagcaggct
    13921 tgcccgacaa accggaacat ggaactcata cggccactcc tttttcaggc tgtcggcagc
    13981 gtgccgcgaa atcgcgagaa cggctatcga ttccggatca acgccttctt gagggaggcg
    14041 aacagcgcat ccgatctggc cgacggtttt ggattggggc tggagcagcg gcagaaaacc
    14101 tgcgcgattc acgggttttc gtaggcgtgg ctcacaggga tgagcgcgaa ccagaggccc
    14161 tcgcagtgga tggaaatcgg ttggccccga gcgacgtacc gttcacccgc aggccaggtg
    14221 cgttggcaca aacggactcc acgggctcgg caggccgtgg gagaatggct tgatccggta
    14281 tggttcaggt tcgcgcgatg cttcagacac gggaagaaac agcaattcgt tacccgggat
    14341 tcgggaagct cctgctggga tgggcggtgc tggccggggt ggcgtgcatt cgccacgtac
    14401 tgctggacaa cgtgccccat gcgcacctgg cacgcgagtt cgccgactgg cttacctgct
    14461 atctgccctg ggttcttctc acgccggcag tgtttcgcct cgaacgcaaa tttccgctga
    14521 acctcgcgca atggcgccgg aatgccgcac tgctgctggc ggcggggctg gtgttgtcgt
    14581 atgcaggcgg gacgatagcg gacgttctgt tttctctgga gcgtattgtg ctggagcctt
    14641 ccgcggcggc cgctccgtgg tggaagatgt cggggagcga attcctgttc cagttcgctt
    14701 tatattgcgt ggctctgctg gccgcctgcc tgatccggaa actaattgaa atgcaggaga
    14761 gcgaacgccg cgcggcgcgg ctaacgctgg agaagtcgga actcgaggcg agcctgcggc
    14821 gatcggagct ggagatgatt cgcatgcggc tgaatcctca tttcctgttc aacagcctgc
    14881 agaatctctc cactctggcg cgacagaatc ccgacgccgc gagcaggatg ctggcgcgcc
    14941 tgggcgatgt gctccgtgcg gcgctgcgca cgagcgcacc ggcacagacg aggctgtcct
    15001 cggagattga gctgacccaa gcctatgtgg aggtggaaca gatccgtttt gacggccggc
    15061 tgtccgttct ttttgaagtg gagcccggca tggacaacgc actggttccg ggattcctct
    15121 tccagccgct ggtggagaac gcgatcacgc atggtttgcg cgatggccaa acctcgggcg
    15181 cgatctgggt acgcgcggtg cgccacgggg aggatttggt gttgaccgta cgggataacg
    15241 gctcaggccc gccggccgac cgactggccg accttgaaat gggcatcggc ctggggtcga
    15301 cctgcgaacg gctggagcgc atgttcggcg atcgccaccg tgtttctgtt aaccggcttc
    15361 cggaaggggg aaccgaggtg cgcatcacgc tgccgctgga atgggccgcg ggtccggctc
    15421 cgagtcaggt gcatgaatac gtttcgagtc ctcatcgttg atgacgagcc gctgatccgc
    15481 gccgggattc gcgacggtct cgcgcgaatg gcggagatcg aaattgcggc cgagtgcgga
    15541 tcgggcgctg aagccctgcg gcagctgtcg tcgctgtcga tcgatctggt gctgctggat
    15601 gtgcaattgc aggattgcac cgggctggac gtggtggagc ggatcggtcc ggaacgcatg
    15661 ccggcggtga ttttcgtgac ggcctacgat gagtacgcgg tgaaggcgtt tgagctgaac
    15721 gcagtcgatt atctgctgaa gccattcgac gacgctcgcc tgcggcgcag tgtcgagcgc
    15781 gcctgccggc gcattgcgga gcatcgccag tccgatcttg ccggccagtt gcaggctctg
    15841 ctgcaggcgc gtctccgcaa atggcccgag cgcatcgttg tccgcgatgg ggaccggttc
    15901 gatttcgtcc ccgtggactc catcgactgg gtggaatccg cgaacaacta cgtccaactg
    15961 cattgcggcc caggccggca ccttctgagc gagagcctca caaacctcga aagcaggctg
    16021 aaccccgcgc gctttgtgcg catccatcgt tgtcgcatcg tgaacctgac acggcttgaa
    16081 gccattcatc cgcttcccgg gggaaccttt gagctggaat taagaaatgg aacgcggtta
    16141 acaacgggaa gacagtaccg gaatcaaatc caggctctgc tgcggtcctg acccactcgg
    16201 tagtggacaa cggatctgcg ttcgctacgg cgcaatccgg tgaaaggcct gcaatgtccg
    16261 cagattctcc agcatcacaa cgcccagcca ctctttttcc gccactttcc aggctgcggg
    16321 atcgcggtct gcccaggacc aggtgagccc ccagctgcca tcggatttct gtatggagat
    16381 gatgtagtcc ggattggcgc tgatcgcctg cttcatttcc ggatagaacg gcgattgcgg
    16441 gctgtggacg aaggtgagcg gcttgacgtt gtaactgtcc cagtgtttcg gatcgagcac
    16501 cacaactgct gccagcacat ctttgagttt gcggacgggg gctgttctct gatctgggtg
    16561 caaataaggc acaagatgga tgacggattc gatatccggc atcggcatgc gtaccggaga
    16621 agcggccagg gactgcaaag actgcgccgt gatgcgcttc aggaacccgg gcggcagcaa
    16681 cgccgtatag ctctgcaggt atgctgcaag cgctgctgtt ggaaacagtg aactctccac
    16741 ctggcacttc cctgcgtgca gcgaaacgat gcaggttgtg cccgatggct gcgttgatat
    16801 cgtgtggagc gggggaacac tctccgtcgc gggtcccgac acccggccca ttctctcgca
    16861 cacatcccgc catggcgtcg tagtgggagt gcgcttccag cccggcgcgg ctgccgcctg
    16921 gctgcgccag ccgctggatg ctgacgtgca ttcaaatcga tcctcgggca ggcgtcggtg
    16981 atcctgcgtt actgacttgt actcaactca agcagaagcg caatgcggat ttcggcgagt
    17041 cctcttccag tgaccccttc gttgagacag tttcgatcac cacactcgcc ccgcttacga
    17101 tcctgaaccc tcacctgacg cagctcagag ctcataccga ttgtttagct ttcactgacg
    17161 ccacgtgttt gacgctcgat acccgtgtgg acttcgggaa gcattggcct ctgagttctg
    17221 ccgtccgtgc cctttaagct ttctgctgac cagacgcgaa aaactgcacg caggagcata
    17281 tagttgcgcc ttagtcttgc cgtgggtttg agcttcgtat tcctccggcg tttggtaccc
    17341 caaggcggaa tgcagtcgtt ttaggttgta atagccgtcg atgaactcct cgatgtgact
    17401 gtgcaaatcc tcgaggtcgc ggtatttgtt ggcgtagatt tcctcacgct tgagcgtctt
    17461 gataaagctt tcgcaactgg cgttgtcgta cggattcgcc ggccgactca tgctctgaat
    17521 catgccgtgg cgcttgagaa tcgcgacata ttccggcgaa acatactgga ctccgcggtc
    17581 ggagtggtgc accacaccgg gcagcggctg tcgcagatcg atcgcgcgct caagcgcttc
    17641 gaccgccagg cgggaggtca gcgcacgatc cagcttccag cccagcacct tccgcgaata
    17701 gccgtccaaa acaatcgcaa ggtagacgaa ttcgccctgc aatcggatgt acgtgatgtc
    17761 cgccacccac agctgatcga ctgaattcgg ccgcatgcgc cgcggcagat tcaggtagac
    17821 ttccagcgca tcgtaagaat ccgcgcagct cacaagtttc cccggctgca gggcgagaag
    17881 attatcctca cgcataattc gcgcaacgcg cttgtggttc acgtgcatcc cgcggtgctt
    17941 cagttccgcc gtgacccgcc ggtagccata tcgacggcgg tgctcgagcg ccacccgctg
    18001 aatggccgac cgcacttcgg tatcctcttc acggggtacg ctggctttca ggaaccggta
    18061 gaaccccgcc cggctcactc tcgccaggag gcacattcgc tcgacgccaa ggctgccttg
    18121 catcggcatc acttcccgga tcaggtcgta gatgccgtcc cgccagagca tgtgctgctc
    18181 tggcgtcgag cctcgacttt ttgcaaggcg cctttgaaaa aatccacctc cagcgtcttc
    18241 tccgcaagca cgcgctgcaa ctcacgcacc tgcttgcgga gttcacgaac cggggatgta
    18301 accctggcgc tcgtcccact gccgtcctca agctgccgct gccactcata caaattgctc
    18361 cggtcgatac ccagctcttt cgccagagca gacacgcttg gacaatcctt catccgctcc
    18421 acggccatgt gccggaacga taaagggtat ttgccgactc ttctctgcgc cagttgcagt
    18481 tccctccccc gcaaagtgtt ggaaggaata gtgcctcact atggggtcag tccacgggca
    18541 attttgaagg tcatcgggga gtgtacggga ggcgtaagtc gctgaaaaag attggctcct
    18601 caggtaggac tcgaacctac aacccttcgg ttaacagccg agaacgtggc cttttacgag
    18661 ctgctaccga ttgagataag tcaatacaga ttaccagttc agagctattt tctctcccct
    18721 ccatgaacgc tggaatgcga ccaaatactg aggtggctac ggcacacaat ccaccacacg
    18781 ccaataaggt ttggtcgaga tttcgcgcct gcttttcacc atgtgagctt agcttacgac
    18841 aac
//
LOCUS       DASZYN010000547         2856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_475565, whole genome shotgun sequence.
ACCESSION   DASZYN010000547 DASZYN010000000
VERSION     DASZYN010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475565"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1085)
                     /locus_tag="VHE33_14185"
     CDS             complement(54..1085)
                     /locus_tag="VHE33_14185"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT98647.1"
                     /translation="MHLTALRSAIHPTILVLAAFCLFQSPAAPEDAPLPPGKLVELGG
                     HRLHVNCTGQGRPVVVVENGLGDFSFDWILVQQRVARFTRICTYDRAGYAWSDAGPMP
                     RTFAQINLELREALRVLGERGPFVLVGHSYGGPVMRNFATTWPAEVAGMVFVDAAWEG
                     MRVQIGEKQTVRLGGSGQERAVPKPHERWTASDKDALRAMAEPPHPAEPLDPLYQKLP
                     EAEQRLQQWAQNLPNLEPTEASQRQWSEVDFARLLAHPEPHALRSIPTIVLSRSEGGY
                     TSDLDVPAAQMEQERKDGQAMLARLSKKSKQVFLHCGHNMEIEDPAGVADAIQQAVEA
                     ARHRGNLQT"
     gene            1357..2328
                     /gene="msrP"
                     /locus_tag="VHE33_14190"
     CDS             1357..2328
                     /gene="msrP"
                     /locus_tag="VHE33_14190"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HVT98648.1"
                     /translation="MLIRTKGEIPSSEITPKSAFMNRRKFMAAAAATGIAAVAGETAS
                     RILRPSTAEAADEKLTTVKSQYSTTGVTLTSFENITHYNNFYEFGVNKDDPAKNAWRL
                     KTRPWTIQVEGLVKQKKTFDIDTLLKMKPLEDRVYRFRCVEAWSMVIPWVGYSLSEFI
                     NACQPLPSAKYVQFISLDDMKQELLPWNAGISWPYSEGLRMDEAMHPLTLLTFGLYGQ
                     VLPNQDGAPVRVVVPWKYGFKSAKSIVKIKFVKNQPSTTWNEMASNEYGFYSNVNPNH
                     DHPRWSQKTERVIGAPIFKQRQPTLMFNGYQDQVASLYNGMDLNKDY"
     gene            2369..>2856
                     /locus_tag="VHE33_14195"
     CDS             2369..>2856
                     /locus_tag="VHE33_14195"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HVT98649.1"
                     /translation="MNKRGIVVLKLLAHAACLAPIAWLFWHLYLSVTSDPGALGPDPT
                     HTVTFFTGRGTLRLLVITLAVTPVRRLIPKLAWLGRFRRVLGLYAFFYGCLHLLTYVW
                     LYAGWSWAAIVDDISQRRFITAGVAAWLMMLPLALTSTTWSIRKLGGKNWNRLHWLTY
                     AAA"
BASE COUNT          566 a          965 c          803 g          522 t
ORIGIN      
        1 agaacttcag cgcagacgtt caggcgctgc gtcgaacgtc ataccgtcgc ccttcaggtc
       61 tgaagattcc ctcggtggcg tgcggcctcc accgcctgct ggatcgcatc cgccacgccg
      121 gccggatcct caatctccat attgtggccg cagtggagaa agacctgctt gctcttcttc
      181 gaaagacgcg ccagcatcgc ctgcccatct ttgcgttcct gttccatttg tgccgccggc
      241 acgtcgagat cgctcgtgta accgccctcg cttcggctga gaacgatcgt cggaatcgat
      301 cgcagcgcat gcggctccgg atgagcgagc agccgcgcaa aatcgacctc cgaccattga
      361 cgctggctcg cttcggtcgg ttccagattc ggcagattct gcgcccactg ctgcaaacgc
      421 tgctcggcct ccggcagttt ctggtacagc ggatcgagag gttcggccgg atgaggcggc
      481 tccgccatcg ctcgcagagc atccttgtcg ctcgcggtcc agcgctcatg cggcttgggt
      541 acggcgcgct cctgcccgct tccgcccagc cgcaccgtct gcttctcgcc gatttgcacc
      601 cgcatgccct cccacgctgc atcgacgaac accatgcccg ccacctccgc gggccatgtc
      661 gtcgcgaagt tcctcatcac cggtccgcca tatgaatggc cgacaagaac aaacgggccg
      721 cgctcgccga gaacccgcag tgcctcgcgc aattccagat tgatctgagc aaaggttcgc
      781 ggcatcggcc ccgcatcgct ccacgcatat cccgcgcggt cgtaggtgca gatgcgcgtg
      841 aagcgcgcga cgcgctgctg caccagaatc cagtcgaacg agaagtcgcc gagtccgttc
      901 tccaccacca ccacggggcg gccctggccg gtgcagttca catgcagacg atgcccaccc
      961 agctccacca gcttgcccgg cggcagggga gcgtcctctg gcgcagcagg tgattgaaac
     1021 aggcaaaaag cggcgagaac gagaatcgta gggtggattg cggatcgcaa agcggtcaga
     1081 tgcatcttca tctccgggtc actggctttg cgcagaatac ggactcgcgc cgctgcctgc
     1141 tcctccaatg acgcaaagtg gcctcgccac gggaggaagc gcatcggccg ggggatgaag
     1201 cgtccgggga tcacgaggca ttcccaacaa tccgtgggcg ccgcgagatg cgtactcctc
     1261 tgtgatgacg caacgggagg cgagtggcgt aacgtcaaag atgtctcctg ttctccggct
     1321 ggtgcgtcaa ataaggagag gggtagagta ggaaacatgc tcatacgcac gaaaggcgag
     1381 attccctcgt cggagataac gccaaagtcg gccttcatga accgccgcaa attcatggca
     1441 gcggcagcgg ccaccggcat cgctgctgtc gccggcgaaa ccgcgtcccg catccttcgc
     1501 ccttcaactg ccgaagcagc cgatgaaaag ctgaccacgg tgaagagcca gtactccacc
     1561 accggggtca cgctcacgtc cttcgagaac atcacgcact acaacaactt ctatgagttc
     1621 ggcgtgaaca aggacgaccc cgcgaaaaac gcatggcggc tcaagacgcg cccctggacc
     1681 atccaggtcg aaggcctggt caaacagaaa aagaccttcg acatcgacac gctgctgaag
     1741 atgaagcccc tcgaggatcg cgtctaccgg ttccgctgcg tcgaggcgtg gagcatggtc
     1801 atcccctggg tcggctattc gctgtcggaa ttcatcaacg cctgccagcc cctgccttcg
     1861 gcaaaatacg tccagttcat ttcgctcgac gacatgaagc aggagctgct cccctggaac
     1921 gccggcatca gctggcccta ctccgagggc ctgcgcatgg atgaagccat gcatccgctc
     1981 acgctgctca ccttcggcct ctatggtcag gtcctgccaa atcaggacgg tgcgcccgtc
     2041 cgcgtcgttg tcccctggaa gtacggcttc aaatccgcga agtcgatcgt gaagattaaa
     2101 ttcgtcaaga atcagccctc caccacgtgg aacgagatgg cgtccaacga atacggcttc
     2161 tactccaacg tgaatcccaa ccacgatcat ccgcgctgga gtcagaagac cgagcgcgtc
     2221 atcggagccc cgatttttaa gcagcgtcag cccacgctga tgttcaacgg ctatcaggac
     2281 caggtggcga gcctgtacaa cgggatggac ctgaacaagg actactgaac gccgcaccca
     2341 cagcagggag caaagacaga accccggaat gaacaagcgc ggaatcgtag tcctgaagct
     2401 cctggcgcac gcagcctgcc ttgcgcccat tgcctggctt ttctggcatc tctatttgtc
     2461 ggttacctcc gaccccggcg cgcttggccc ggatcccacc cacaccgtga cctttttcac
     2521 cggacgtgga acgctgcgcc tgctcgtcat cacgctcgcc gtcacacccg tacgccggct
     2581 gattccaaag ctcgcctggc tgggacgatt ccgccgcgtg ctcggcctct acgccttctt
     2641 ctatggctgc ctgcacctgc tcacgtatgt gtggctctat gcgggctgga gctgggccgc
     2701 gatcgtcgac gacatcagcc agcgccgctt catcaccgcc ggcgtcgccg cgtggctcat
     2761 gatgctgccg ctcgcgctca cctcaaccac gtggtccatc cgcaagcttg gcggcaaaaa
     2821 ctggaaccgg ctccactggc tcacctacgc agccgc
//
LOCUS       DASZYN010000548         2514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_345981, whole genome shotgun sequence.
ACCESSION   DASZYN010000548 DASZYN010000000
VERSION     DASZYN010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345981"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VHE33_14200"
     CDS             complement(<1..863)
                     /locus_tag="VHE33_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98650.1"
                     /translation="MRYFRYERPITGVAKAGQTCVALDAGTYAHAEPGLGDVRLFRDG
                     KETPYAIRDSAPVEQQRGEIAPLNLGHKGAHTTFETEMPAGRYSDVDLDITAKDFIAT
                     VAVTGAQDETGREGTELGLFTIFDLTAQKLGRSTVLHLPESNLKYLYFRIDGPLKPGD
                     VRGLSVERVPAKRQYVTVADTNQGSQKDRNSIFTFHVPAHVPVTRVEFVVGAEPVNFS
                     RNVTVRAKPAPTGKLNTEEEPPRPVESSGTLLRLHTTREGHPIDEEDLAVAAPWVDLR
                     DAESTWTVTVD"
     gene            complement(931..>2514)
                     /locus_tag="VHE33_14205"
     CDS             complement(931..>2514)
                     /locus_tag="VHE33_14205"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HVT98651.1"
                     /translation="IGSQWFNRIGILAVLIAAAWFLKLAIDNHWIGPAGRVLIGLVAG
                     AGLIAWSERFRTRGYAAFSYSLKAIGSGVLYLSLWAAFSLYHLMGSGVAFAAMIVVTA
                     FNGFLSWVQDAELLALYAIVGGLSTPLLLSTGVNHEVALFTYLLLLDLVVLALVALKP
                     WSRLLCAAFLGTVFFFLSWSFEYYRTDEFGTTAFFLAVFFLIFAWAPRLVRLRVEDGG
                     AHATWDNLALVAVPLINAGLGFLGYYVMLEDAHSKAAPWVAVAFAAFYLLLLRLPARG
                     AIHGSAAATSALHLTIAVVFLTIAIPLKTQGRWMTIGWLAEGAALVWLARRTASTLLT
                     ALALLCLVLGLGALVAANPTASLTPVFNERFGTYLFGIVVCGFVTWLAMQAAGETGPE
                     PAIGWKSIAAGAGLAVNALILLAVGWEIHAYWWYMQWRGDWTLMHDYRMYAQFTYSAF
                     FMLFGAALLALGFIRRVAFLRWQALVLLAVAIGKVFIVDVSTLSQGYRILSFLGLGAL
                     LLAVSFAYQKDWLNLRKAN"
BASE COUNT          544 a          964 c          606 g          400 t
ORIGIN      
        1 ttgtccactg tcaccgtcca cgtcgactcc gcatctctca aatccaccca cggtgccgcg
       61 accgccagat cctcctcgtc gatcgggtgc ccctcccgcg tcgtgtgcag ccgcagcagc
      121 gttcccgacg actccaccgg ccgaggcggc tcctcctctg tgttcagctt gccagtcggc
      181 gccggtttcg cccgcaccgt gacgttgcgg ctaaagttca ccggctccgc gcccaccaca
      241 aactccaccc gcgtcaccgg cacgtgcgcc ggcacgtgga atgtgaagat cgaattcctg
      301 tccttctgcg acccctgatt cgtatccgcc acggtgacgt attgccgttt cgccggaacc
      361 cgctctaccg acaggccgcg aacgtccccc ggcttcaatg gcccatcgat cctgaaatac
      421 aggtacttca gattcgactc cggcagatgc agcaccgtgc tccttcccag cttctgcgcc
      481 gttaaatcga aaattgtgaa cagccccagc tccgtgcctt cgcgcccggt ctcatcctgc
      541 gctcctgtaa cagccacggt tgcaataaag tccttcgccg tgatgtcgag atcgacgtcg
      601 ctgtaccttc ccgccggcat ctccgtctcg aaggtcgtat gcgcgccctt gtgccccaga
      661 ttcagcggcg caatctcccc tcgctgctgc tccaccggcg ccgaatcgcg aatcgcatac
      721 ggcgtctcct tgccgtcgcg aaacagccgc acatccccga gccccggctc cgcgtgcgca
      781 taggtccccg catccagcgc cacacatgtc tgacccgcct tcgcaacccc cgtgatgggt
      841 cgttcatacc ggaaatacct catctccggc gttgccactg ccaatagcaa caagaccatt
      901 cgcccacgcc aacccatcag caccagaccc tcaattcgct tttcgcagat tcagccagtc
      961 tttctgatat gcgaagctca ccgccagcaa caacgccccc agtcccagga aactcaaaat
     1021 ccgataaccc tggctcagcg tgctcacgtc cacgatgaac accttgccga tcgccaccgc
     1081 cagcagcacc agcgcctgcc accgcaaaaa agctacccgc cgaatgaatc ctagcgccag
     1141 cagcgctgcg ccaaacagca tgaaaaacgc cgagtaggtg aattgcgcat acatccggta
     1201 gtcgtgcatc agcgtccagt cgccgcgcca ctgcatgtac caccagtagg cgtgaatctc
     1261 ccagcccaca gcaagcagga tcagcgcatt caccgccagt cccgcccctg ccgcaatgct
     1321 cttccaccca atcgcgggct ccggccccgt ctcacctgct gcctgcatcg caagccatgt
     1381 cacaaatccg cacaccacaa tcccaaacaa atatgtcccg aagcgctcgt tgaacaccgg
     1441 cgttaacgac gccgtcggat tcgccgccac cagcgccccc agccccagca ccaggcacag
     1501 cagcgccagc gccgtcagca atgtcgacgc ggtgcgtcgc gccagccaca ccagcgccgc
     1561 gccttcggcc agccagccga tcgtcatcca ccgcccctgc gttttcagcg gaatcgcgat
     1621 cgtcaggaac accaccgcaa tcgtaagatg cagtgccgat gttgccgcgg cactcccatg
     1681 aatcgccccg cgcgccggca agcgcaacag caacaagtag aacgccgcga acgccaccgc
     1741 cacccacggc gccgccttgc tgtgggcatc ctcgagcatc acgtaatagc ccaggaagcc
     1801 gagtcccgca ttgatcagcg gcaccgccac gagtgccagg ttgtcccatg ttgcatgtgc
     1861 gccgccatct tccacgcgca gtcgtaccag ccgcggcgcc cacgcaaaaa tcagaaagaa
     1921 tacagccagg aagaaggccg tcgtcccaaa ctcgtccgtc cggtaatact caaacgacca
     1981 gctcaggaag aagaacaccg tccccagaaa cgcggcgcac agcaggcgtg accacggctt
     2041 cagagccacc agcgccagca ccaccaggtc cagcaacagc aagtaagtga aaagagcgac
     2101 ctcgtggttc acgcccgtcg acagcaacaa gggtgtcgag aggccgccca caatcgcata
     2161 cagcgccagc agctccgcat cctgcaccca cgacaggaat ccgttgaacg cggtcaccac
     2221 gatcatcgct gcaaacgcca cgcccgaccc catcaggtga tagagcgaaa acgccgccca
     2281 cagcgataag taaagaacgc cgctgccgat cgccttcagt gaatacgaga atgccgcata
     2341 cccgcgcgtc ctgaaccgct ccgaccatgc gatcagcccc gcgcccgcca ccagcccgat
     2401 cagcactcgc cccgctggcc cgatccagtg gttatcgatc gccagcttca ggaaccacgc
     2461 cgccgcaatc agcaccgcca aaattccgat gcggttgaac cactgcgacc cgat
//
LOCUS       DASZYN010000549         3497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_345996, whole genome shotgun sequence.
ACCESSION   DASZYN010000549 DASZYN010000000
VERSION     DASZYN010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345996"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..627)
                     /locus_tag="VHE33_14210"
     CDS             complement(154..627)
                     /locus_tag="VHE33_14210"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT98652.1"
                     /translation="MIQLRSLFESHLTVANLDRAMSFYGKTLGLELATVFEERRVAFY
                     WIGGRGHSMLGLWEVGTAPQRMSLHVAFHVVLENLLQAPAQLRAAGVTPLDFDGRPTD
                     EPVVLAWMPAASLYFRDPDGNMLEFLAMLSDAPQPELGIVPWRRWKGASWDSRTV"
     gene            740..1075
                     /locus_tag="VHE33_14215"
     CDS             740..1075
                     /locus_tag="VHE33_14215"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVT98653.1"
                     /translation="MATSTITRTPLLKAVFGDRNVNRVDVREIRFAPGQRTTRHLHPC
                     TVVGYIAEGNAILEIEGQPAQNLPTGSAFHEPAGTVITDFRNASDAEPLTFIAFYLLD
                     DDQDLITML"
     gene            1149..1592
                     /locus_tag="VHE33_14220"
     CDS             1149..1592
                     /locus_tag="VHE33_14220"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HVT98654.1"
                     /translation="MPVLRTCTHCNKTNRIPARHLADTGRCGNCKNPLPAVAEPLAVG
                     PAEFDEIVREARVPVLVDFWAAWCGPCRMAAPHVAETARDLAGRAIVLKVDTEQHPQL
                     AARYHVQGIPNFAVFSGGNLAFQQAGLVDANTMKSWLSRAAAQAV"
     gene            complement(1677..2030)
                     /gene="rplS"
                     /locus_tag="VHE33_14225"
     CDS             complement(1677..2030)
                     /gene="rplS"
                     /locus_tag="VHE33_14225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HVT98655.1"
                     /translation="MSIHPVMQRLAEKLTRTDLPPFAPGDTVRVQVRIKEGDKERLQA
                     FEGVVIARKNGPQGSFTVRKMSFGQGVERIFPFNSKVIDKVERVRSSKVRRARLFYLR
                     ALRGKAARLKEVERA"
     gene            2347..>3497
                     /locus_tag="VHE33_14230"
     CDS             2347..>3497
                     /locus_tag="VHE33_14230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HVT98656.1"
                     /translation="MLPWVAVLFSIRTPALHGTPVALNFIFIAGITVFSELGPGLLAV
                     LLTTIFFNHLALARDHFFSLTPQSLLYSTVILTIGALIAFLCDRQRFTSGELRAALAS
                     LQIRTNALAEAQQASRFAAWMHNAETGRIEWADGGAEIFGRPYSETPVSDDWTNLIID
                     EDRARVLQTVYAATSVGQGFRAEFRVRWPNGELHWLETRGTPSAANPKIWHGVTIDIT
                     DRKNAESALVRSEKLAAIGRLSATIAHEVNNPLEAITNLIYLAKIDPNLSPQTSAYLQ
                     RADQELARLGAIARRTLTFVRARSAPGPVSVFETIDSVAGMFQPRCAARSAEILVVPF
                     SDFSVSISGDDLRQILTNIVSNACDALPESGGMIRIDAACADGKATITVA"
BASE COUNT          598 a         1304 c          924 g          671 t
ORIGIN      
        1 gacctcatcg ttggggagcc gtttcctgcc ccgcttcggg atcagctcgc cctgagcctc
       61 cccctgaact tccctgatgg tcgcgcccgc gctgcccata gggggagtct atcgccctcg
      121 cgcgcgagcg tgctcgtgga aaacgcgtcc cgctcaaaca gttcgcgaat cccagctggc
      181 acctttccag cgccgccatg gaacgatgcc caactcaggt tgcggcgcgt cggaaagcat
      241 tgccaggaac tccagcatat tgccatcggg atcgcgaaag tacagcgacg ccgccggcat
      301 ccatgcaagc acgacgggtt catcggtcgg tcgtccatcg aaatccagag gagtaactcc
      361 agccgcgcgc agctgcgcag gcgcctgcaa caaattctcc aacacaacat ggaaggccac
      421 atgaaggctc attcgctgcg gagccgtgcc cacctcccac agcccgagca tcgagtggcc
      481 gcgcccgcca atccagtaaa acgcgacccg ccgctcctcg aagaccgttg ccagctccag
      541 ccccagcgtc ttgccgtaga acgacatcgc acggtcgagg ttcgctaccg tcaagtggct
      601 ttcgaacaga ctccgaagtt gaatcatgac tgactctccg tcgttggatt cgcatgcttg
      661 ccaatcgact cccgaattgc cacgacaagc acttctggcg acgctgaatc cgggtcagcc
      721 gcgcccacat cggataggtg tggccacctc gaccatcacc cgcactcccc tgctgaaagc
      781 ggtctttggc gatcgcaacg tcaaccgggt cgacgttcgc gagattcgct tcgccccagg
      841 ccagcgaacg acgcgccatc tccatccgtg caccgtcgtc ggctacatcg ccgagggcaa
      901 cgcgatcctc gagatcgaag gccagcccgc gcagaacctt cccacgggtt cggcgttcca
      961 tgagcctgcc ggaaccgtga ttacggactt ccgcaacgcc tcggacgccg aacccctgac
     1021 cttcatcgcc ttttatctgc tggatgacga ccaggatttg attacgatgc tctagacgac
     1081 tacggttcct gggttcctcc ctgggttcct cccaggcttc ctttgcatcc atatactgga
     1141 gccccgccat gcccgttctg cgcacctgta cccattgcaa caagacgaac cgcatccccg
     1201 cccgtcatct cgccgacacc ggccgttgcg gcaactgcaa aaatccgctt cctgctgtgg
     1261 ctgagcctct tgctgtgggc cccgccgagt tcgacgagat cgtccgtgaa gcccgcgtcc
     1321 ctgtgctggt cgatttctgg gccgcctggt gcggaccctg ccgcatggcc gctccgcacg
     1381 tcgccgaaac cgcgcgcgat ctcgccggcc gcgccatcgt cctcaaagtc gacacggagc
     1441 agcatccgca gcttgccgcc cgctaccacg tccagggcat ccccaacttc gcggtcttca
     1501 gcggcggcaa cctcgccttc cagcaggccg gcctcgttga cgccaacacg atgaagtcct
     1561 ggctcagccg cgccgctgcg caagcggtct gacacacgaa cgccccggct ctgcaccggg
     1621 gcgcctgggt cttctgaacg ccgaacgcca gctttttcag aacgctgaac gctggcttat
     1681 gcccgttcca cttccttcag cctggcagcc ttgccgcgca gtgcccgcag gtagaacagc
     1741 cgcgcccgcc gtaccttcga cgaccggact cgctcgacct tgtcgatcac cttgctgttg
     1801 aaggggaaaa tcctctccac gccctggcca aagctcatct tgcggacggt aaagctgccc
     1861 tgcggcccgt tcttgcgcgc gatcaccacg ccttcaaagg cctgaagccg ctccttatcg
     1921 ccttccttga tccggacctg cacgcgcacg gtatcccccg gcgcaaaggg cggcagatcg
     1981 gtgcgggtca gcttctcggc aaggcgctgc atcactggat gaatcgacat gactaaactc
     2041 gtttctgaag aggaatatgc gaatccccag tttaacaaag atttcgcatt tgccgcgccg
     2101 ccccgtggcc ggtccaggct cgccctgcgc taaccggttc atcgctgacc tccttacctg
     2161 ccggccggct cctggctggg gcgctgttgt gtatcctgtc taacattgcc gccgggaccc
     2221 caggttcccc acccgtccgg tgcgcagtcc gcagcttgag gccgcaattt gacccgaccc
     2281 ccacgtccag gcacgtctcg ctcatcccgt ctcctggttc gaatctgtgg ttatattgcc
     2341 gcggtcgtgc tgccctgggt ggccgtcctg ttcagcatcc ggactccggc gctgcacgga
     2401 actccggtcg cgctcaactt catcttcatc gccggcatca cggttttctc cgagctcggc
     2461 ccgggcctgc tggccgtcct cctcacgacg atcttcttca accatcttgc cctggctcgg
     2521 gatcactttt tctccctgac tccccagtct ctcctctact ccaccgtcat cctcaccatc
     2581 ggcgcgctca tcgctttcct ctgcgaccgg cagcgcttca ccagcggcga gctccgcgcc
     2641 gccctcgctt ctctgcagat tcgcaccaat gcactcgccg aagcgcagca ggccagccgt
     2701 ttcgccgcct ggatgcacaa tgccgagacc ggacgcatcg agtgggccga cggcggcgct
     2761 gagatcttcg gccgccccta ctccgagacg cctgtctccg atgactggac caacctcatc
     2821 atcgatgagg atcgggcccg cgtgctccag acggtctacg cggcgacttc cgtgggccag
     2881 ggctttcgcg cggaatttcg tgtccgctgg cccaacgggg aactccactg gctcgagacg
     2941 cggggcacgc cctcagcggc caacccaaag atctggcacg gcgtcaccat cgacatcacc
     3001 gatcgcaaaa acgccgagtc ggcccttgtc cgctccgaga agctcgccgc catcgggcgg
     3061 ctctccgcga ccatcgctca cgaggtcaac aacccgctcg aagccatcac caaccttatt
     3121 tatctggcga agatcgatcc gaacctttcg ccgcaaacca gcgcatacct ccagcgcgcc
     3181 gatcaggagc tcgcccgtct gggcgctatc gcgcgccgca ccctcacctt cgtgcgcgcc
     3241 cgctccgccc ccggccccgt cagcgtcttc gagaccatcg attcggtcgc cggcatgttc
     3301 cagcctcgtt gcgccgcccg cagcgccgaa attcttgtcg tccctttttc ggacttcagt
     3361 gtttccattt ccggcgacga cctccgccag attctcacca acatcgtctc caacgcctgc
     3421 gacgctctgc ccgaatccgg cggcatgatc cgcatcgacg cggcctgtgc cgacggcaaa
     3481 gccaccatca ccgtcgc
//
LOCUS       DASZYN010000550         3357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_88274, whole genome shotgun sequence.
ACCESSION   DASZYN010000550 DASZYN010000000
VERSION     DASZYN010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88274"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VHE33_14235"
     CDS             <1..583
                     /locus_tag="VHE33_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98657.1"
                     /translation="ALLYYYFRDKEGLYAAALEEVAGKVAGDALAVLDLNCSAGEKVL
                     RTALQHFDRILSQNRFQALMQQEMIRFRTGQSNAMQILADKAFAPTWDRTLQTVKEGI
                     SSGELCDVDPMQMLYGAIGANVFYFLSAPMVHLVSAKDPLDPAAVAARRTAAIEYLSQ
                     TIFTDRKRGARLAKAVLAAVPMPQPANAERKSA"
     gene            580..1644
                     /locus_tag="VHE33_14240"
     CDS             580..1644
                     /locus_tag="VHE33_14240"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVT98658.1"
                     /translation="MKPRNRIFIIMAVLLVIALAWYFFSVNHTSDLQLIGTVDANEVI
                     VSSRIQGRIAKLAVDEGQKVTAGQLVATIEADDLAALRNAAEATVAGQRYKLAGSRDT
                     EQQTQGNTSAQVTAAEAQLRVAKAAQAQAQAQYEHQEADTKRAVALAQAGVASEQTRD
                     EMVTSLNAAKAAVDSARENVAAAEATLKTAQANVLESQAAAKTVAANRADVRNAQQLL
                     DQADVQLGYARVISPVTGIVNVRAAREGEVVSPATPIVTVVDLSQTWVYAPLPETEAD
                     AVQLGDKLRVVMPSGATVEGKVIAKSAEADFATQRDVSRRKRDIKTIQLKLLVDNPGM
                     RFVPGMIADVYVPKDKLVKE"
     gene            1641..2687
                     /locus_tag="VHE33_14245"
     CDS             1641..2687
                     /locus_tag="VHE33_14245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVT98659.1"
                     /translation="MTGSAKKLHAGVPPASPPPAIAVENIVKRYGTFEAVSGISFEVA
                     DGEIFGLLGPNGAGKSTLIRMMTTLIPVTAGRALISGHDVVHEPNAVRRLIGVIPQAL
                     TSDIDLTVEENLSIYAKLYGVPHAERERNIAELLEAVDLTKWREAQTKTLSGGMRRRL
                     EIARGLVHSPKIFFLDEPTTGLDPVSRVAVWEMLDRLRTRMKLTILITTHYMDEADNL
                     CDRIAIIDHGKLVALNTPLQLKSSVPGSSVIEVQFGRNQVDRKPSLEKLGAVTGVESR
                     GSGEYRLMTSNGSLTTTQLVEMATATNDPIQGLSVQNTTLDDVFVYYTGRQLRDEQVK
                     AVSFVMPPRPGMQP"
     gene            2684..>3357
                     /locus_tag="VHE33_14250"
     CDS             2684..>3357
                     /locus_tag="VHE33_14250"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT98660.1"
                     /translation="MNRMWAIVEREMRKFFRSPTLMLMSMTLPLVQLIILGNAFGGKV
                     TNARMGVVDYDHGEEALKVHEAFQSVAANIRTFETIAYQDEQTARDDVRTGRIDAAVI
                     IPAQYSRRVLAGDSPHIGLVVDNSDQFVSGSLESEMQSLVDALNAPLIQPKVVQAISL
                     NVVELYPYIEYMKFLLAGSVALAMYISVMIGGGMLYIDDKARGVHEGYLVTPITKLEL
                     VLGLNIA"
BASE COUNT          710 a          952 c         1078 g          617 t
ORIGIN      
        1 ggcgctgctc tactactact ttcgcgataa agaaggcctt tacgcggcgg ctctggaaga
       61 agtcgcgggc aaagttgccg gcgacgcgct tgccgtactg gatttgaatt gcagtgcagg
      121 ggagaaggtt ctgcgcaccg ctctgcagca cttcgatcgc atcctctcgc agaaccgctt
      181 tcaggccctg atgcagcagg aaatgatccg cttccggacc ggtcagtcca acgcgatgca
      241 aatcctcgcg gacaaggcat ttgcaccgac gtgggaccgg accctgcaaa ccgtgaagga
      301 agggatcagt tccggggagc tctgtgacgt tgatcctatg cagatgctgt atggagcgat
      361 tggcgcgaac gttttctatt tcctgagcgc accgatggtt catctggtca gcgccaagga
      421 tcccctggac ccggctgcag tggcggcccg acgcaccgcg gcaatcgaat atctgagcca
      481 aaccatcttt acggatcgca aacggggcgc acgcctcgcc aaagccgtgc tggcggcagt
      541 tccaatgccg cagcctgcca atgcggagag aaagagcgca tgaaaccacg caatcggatt
      601 ttcatcatca tggcggtgct gctcgtcatc gcgctggcct ggtatttctt ctcggtgaat
      661 cacaccagcg acctgcagct gatcggcacg gttgacgcca acgaagtcat cgtcagctcc
      721 cgcattcagg ggcgcattgc aaaactggcc gtggacgagg gtcaaaaggt cacggctggt
      781 cagctcgtag ccaccattga ggccgatgat cttgctgcat tgcgtaacgc cgcggaagcg
      841 accgttgctg gtcagcgcta caaactggcc ggttcgcgcg atacggaaca gcagacgcag
      901 ggcaatacct ccgctcaggt gacggccgcc gaagcccagt tgcgggttgc gaaagcggca
      961 caggcgcagg cccaggcgca gtacgaacac caggaagccg atacgaagcg cgcggtggcc
     1021 ctggcgcagg ctggagtggc cagcgagcaa acgcgcgacg agatggtgac cagcctgaac
     1081 gcggcgaagg cggcggtgga ttcggcgcgg gaaaacgtag cggcagcaga agccactctg
     1141 aagacggccc aggccaatgt gctggagagc caggcagcag cgaagacggt ggcagccaac
     1201 cgcgccgatg tgcgaaatgc ccagcaactt ctggatcagg cggatgtgca actgggctac
     1261 gctcgggtga tctctccggt gaccggaatc gtgaacgtgc gcgcagcgcg ggagggcgag
     1321 gttgtgtctc cggccacgcc gatcgtgacg gtcgtggatt tgtcgcaaac gtgggtgtac
     1381 gctccgttgc cggagacgga agcggatgcg gtgcaattgg gcgacaagct gcgcgtggtg
     1441 atgccgagcg gcgctacggt cgaaggtaag gtgatcgcga aatccgcaga ggcagacttc
     1501 gcgacgcagc gggacgtgag ccgccggaag cgcgacatca aaacgatcca gctcaagctg
     1561 ctggtcgaca atccgggcat gcgattcgtg cccggcatga tcgcggacgt ctatgttccc
     1621 aaagacaagc tggtgaagga atgaccggct cggcgaaaaa gttgcacgct ggtgttccgc
     1681 cggcgtcacc accgcccgcg attgccgtgg agaacatcgt caagcgatac ggaacattcg
     1741 aggcagtcag cgggatcagc ttcgaagtgg ccgatggtga aatcttcgga cttctgggac
     1801 ccaacggagc cggcaaatcg acgctgatcc gcatgatgac gacgctgatc ccggtcacgg
     1861 cgggacgcgc tctgatttcc ggccacgatg tggtccacga accgaacgcg gtgcggcggc
     1921 tgatcggagt gattccgcag gccctgacca gcgacatcga cctcacggtt gaagagaacc
     1981 tgtcgatcta tgccaagctc tacggcgttc cccatgcgga gcgcgagcgc aatatcgctg
     2041 aactgctgga ggccgtcgat ctgacgaaat ggcgggaggc gcagacgaag accctttccg
     2101 ggggcatgcg acgccgtctg gagatcgcgc ggggcctggt ccacagcccg aagatctttt
     2161 ttctggatga gcccacgacg gggcttgatc ctgtctcgcg ggttgctgtc tgggagatgc
     2221 tcgaccggct gcgcacaagg atgaagctga ccatcctgat cacgacgcac tacatggacg
     2281 aggccgacaa tctctgcgat cgcatcgcga tcatcgatca cggcaagctc gtggctctga
     2341 acacgccgct gcagctgaag tcgagcgtgc cgggttccag cgtgatcgag gtccagtttg
     2401 gcaggaacca ggtggaccgt aaaccgtcac tggaaaaact gggcgccgtg acgggagtgg
     2461 aatcccgggg ctcgggcgag tatcgcctga tgacgtcgaa cggctctctg acgacaacgc
     2521 aactggtgga gatggcgacg gctaccaacg atcccattca gggactcagc gttcagaaca
     2581 ccaccctgga tgatgtcttt gtctactaca cgggacggca actgcgcgac gagcaggtga
     2641 aagcggtcag cttcgtcatg ccgccgcgcc cggggatgca gccatgaacc gcatgtgggc
     2701 aattgtcgaa cgggagatgc ggaagttctt tcgctctcca acgctgatgc tgatgtcgat
     2761 gactttaccg ctggtccagt tgatcattct gggcaatgcc ttcggcggta aagtaacgaa
     2821 cgcccgcatg ggcgtggtgg actacgacca tggcgaagaa gctctgaagg ttcacgaagc
     2881 tttccagtcg gtcgcagcga atatccgtac ctttgagaca atcgcctacc aggacgagca
     2941 gaccgcccgg gatgacgtgc gcaccggccg tatcgatgcc gcggtgatca ttcccgcgca
     3001 atattcacgc cgcgtgctgg ccggggattc acctcatatc gggctggtgg tcgataactc
     3061 cgaccaattt gtcagcggga gtcttgagtc cgagatgcaa tcgctggtgg acgcactgaa
     3121 cgcgccgctg atccagccga aggttgtgca ggcgattagc ctgaacgttg tggagctgta
     3181 tccctatatc gaatacatga agtttcttct ggccggctcg gtcgccctcg ccatgtatat
     3241 ctcagtgatg atcggcgggg gaatgttgta tatcgatgat aaggcgcggg gggttcacga
     3301 aggttatctg gtcacgccga tcacgaaact tgaactggtg ctcggcctca acatcgc
//
LOCUS       DASZYN010000551         9868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_604421, whole genome shotgun sequence.
ACCESSION   DASZYN010000551 DASZYN010000000
VERSION     DASZYN010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9868
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604421"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..873
                     /locus_tag="VHE33_14255"
     CDS             214..873
                     /locus_tag="VHE33_14255"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT98661.1"
                     /translation="MESDSEQIVGLYQRHAAAWDELRSPGSLFEKPWLDKFLAVVPAG
                     GSALDLGCGGGLPISGYLIGQGRAVTGVDSSPPLIDLCRQRFPQQEWIVADMRTVDLA
                     RRFDALIAWDSFFHLCPKDQRRMFVVFSRHAKPGAALMFTSGSHLGEAIGEFQGEPLY
                     HGSLDPEEYENLLEQFGFELVEHVADDPGCGHHTIWLSRRRGLQTDSRRSSSGVQHAD
                     I"
     gene            1008..2330
                     /locus_tag="VHE33_14260"
     CDS             1008..2330
                     /locus_tag="VHE33_14260"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HVT98662.1"
                     /translation="MTQAKSAVVVGAQWGDEGKGKIVDVLSERFTVVARYAGGHNAGH
                     TVIIDGKKFILQLVPCGVLRPGCRGVIGNGVVLDPMAFLKETGRLRELGIDVDSRLFV
                     SNRAQVILPYHRMIELAAESAPGRQKIGTTSRGIGPAYEDKMARNGLRVVDLLNTSLL
                     KTHIENACGEKNAIAHALFGTEPLDPAQMYDEYTAAAEKIRPFVTDTSLLLNDALKEG
                     HSLMFEGAQGTMLDIDHGTYPFVTSSSSAAGGASTGTGVGPTQIGTVIGVTKAYVTRV
                     GEGPFPTEIHDESGEMLRARGQEYGAVTGRPRRCGWLDLPLLRYSNQINGTEWLVITK
                     LDVLDEMDWIPVCTAYEVDGKPCDTMPADIRGIESIRPVYTRLKGWKTSTEGVRSFDD
                     LPRVAQEYLRFMEKETGAKIGIVSTGPDRDQTLEMPEFREAMRRITGK"
     gene            2370..2657
                     /locus_tag="VHE33_14265"
     CDS             2370..2657
                     /locus_tag="VHE33_14265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HVT98663.1"
                     /translation="MISFTVRMKFRSDDRARVAEILTALAQASRREPGCVAYIPHTVD
                     GDADTVVIYEQYRDQQGVDAHRASAHFRDYAVGGLYQLMLDRQVENLTSIA"
     gene            2830..3342
                     /locus_tag="VHE33_14270"
     CDS             2830..3342
                     /locus_tag="VHE33_14270"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVT98664.1"
                     /translation="MRRRPASSLCTSALPQAVAALIVLASCYPAWALPHRDKGEIHKV
                     IESLEEQWRQATVNNNIGEMNRLLADDYVGITSNGTVENKQQALAQRSAGTIRISKLE
                     ITDTHVRVYGDTAVVTSQAQLEGSNGTSDISGQYRYTRVYNRRLGQWKIVSFEASRMH
                     DSDARAGNKH"
     gene            3493..4059
                     /gene="infC"
                     /locus_tag="VHE33_14275"
     CDS             3493..4059
                     /gene="infC"
                     /locus_tag="VHE33_14275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HVT98665.1"
                     /translation="MNDRIRAREVRVIDEKGEQLGILAPFEALKIARERGLDLVEISP
                     TAVPPVCRIQDYGKFLYEKEKSDRAARKRQKVITLKEVKFSVTIDDHDYVTRKNQAVR
                     FLQEGDKVKASLRFKGRQMAHRELGYAIINRLIQDIGENGVVEFMPRMEGNTLHAILA
                     PGKKELPKKPAAPRPQAPPPGPPAQQAQ"
     gene            complement(4135..6603)
                     /locus_tag="VHE33_14280"
     CDS             complement(4135..6603)
                     /locus_tag="VHE33_14280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT98666.1"
                     /translation="MELTTQLKQVLRRLLRAPMFTMVTLITLALGIGATIAVFSVVEG
                     VLLKPLPYPHPEQLVGVWHTAPGLNLDDVNMAPSNYFIYREQNRAFTDIGIYQGDSVS
                     VTGQGSPERITAIDVTDGIFPVLGIAPMLGRTFSKADVAPGAPQTVVLGYGYWQRHFG
                     GDRSVIGRSITADGKIRQIIGVMPRNFRFLDWTIQPGVYLPLQFDRNKTKLGQFSYEG
                     IARLRPGQTIETANTDVARMLPMVWTSFPAPEGFSIELFRKARIGPKIRPLMRDVIGD
                     VGQTLWILLGSIGIVLLIACANVANLMLVRAEGRHQELAICSALGASRWRVMQEFLLE
                     SAVIGVAGAALGLALAWGAVHLLTALAPAGLPRLGDIGIDLPVLGFAVLLALLCSLLF
                     GSIPAFRYGHVSAGTGLREGGRSLSQGRERHRTRNTLVVVQVGLAFVLLICSGLMIRT
                     FRALNHVNPGFDVAGIQTLHIDIPEAEVSDPVQVMRMQETMQHNMAAVPGVTSVSIST
                     SVPLDDGHWMDPVFAQDHSYASGQLPPLRRFRFVTPGFFRTMGIPMIAGRDYTWDEIY
                     QKVPVAIVSEDFAREYWGSPQKALGQRIRVASTDDWREIVGVVGDTYDDGISKEAPKI
                     AYWPLLRNNFEGDKVNIQRWVTFVVRSERAGSESLAGDLRRAIWSVDSNLPVSDVRTE
                     EFLYAKSLARTSFTLVMIGIAAVIALLLGLVGLYGVIAYSASQRRREIGIRIAVGAQR
                     NDITGMFVRQGLLLAGIGVLCGFGVALGVTRLLGSLLFHVSPMDPVTYASACLALVGA
                     AALASYLPSRRTASVDPVEALRAE"
     gene            complement(6752..7021)
                     /locus_tag="VHE33_14285"
     CDS             complement(6752..7021)
                     /locus_tag="VHE33_14285"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HVT98667.1"
                     /translation="MCRNIKPLFNFEPPVTDDEVRAASLQFVRKISGFTKPSQANEAA
                     FEAAIEEISAASSRLLHAMTTGATPKNREEEAAKAKARSAARFPA"
     gene            complement(7029..8297)
                     /gene="glyA"
                     /locus_tag="VHE33_14290"
     CDS             complement(7029..8297)
                     /gene="glyA"
                     /locus_tag="VHE33_14290"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HVT98668.1"
                     /translation="MSRSLPFDDPEVSAAIDAEVRRQHEGLEMIASENFVSRAVLEAA
                     GSVFTNKYAEGYPGRRYYGGCEYADVVENLARDRAKQIFGSEHANVQPHSGSQANAAA
                     YAAVVQPGDTILGLDLAMGGHLTHGHKLNFSGKLYHVVSYGVRKDTETIDYDLLEQTA
                     LREKPKLIIGGGSAYPRFFDFARMREIADKSGALFLVDMAHFAGLVAGGVHPSPVPHA
                     QIVTTTTHKTLRGPRAGMILCRQELAAAIDKTVFPGQQGGPLMHVIAAKAVAFREALQ
                     PSFKTYAAQIVANAKVLAETLASHGYRIISGGTDTHLMLVDVFAKGMLGSEAEAALGE
                     AGITVNKNAIPFDTNPPLKPSGIRIGTPALTTRGMKEPEMRTIGGWIAQALDHRTDSG
                     ALTRIRGQVLEMAEQFPLYGWLREPAGVAG"
     gene            complement(8373..>9868)
                     /locus_tag="VHE33_14295"
     CDS             complement(8373..>9868)
                     /locus_tag="VHE33_14295"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HVT98669.1"
                     /translation="QSPGMLRFWYAPTLFWFSSSSHAMMVVVWAGLIASVLAFANVWP
                     RVSFLVCWVCFLAFVAAAGDFSGYQSDGMLLEAGFLALFVAPNGWWPGWGLDSPPSRV
                     AFFLLQWEWFRIYFESGIVKLLSGDPQWRSLSAMDQYYQNGPLPTFVGWYVQHLPHWF
                     HVGTAAGTLVMELAIVWMLFLPRRVRVICFCIVTPWEIGVILTANYAFLNYLVLFLGF
                     LLLDDRALLSWIPERYRHALEAHVPQESVTMAEERLSVITTPEWEKERAAKKAAAGQD
                     EESEEREVRPGDVWRHVHAVRLAVTAVILTWLGYATTTEMLRMPWPELPLPVFPIAAL
                     EPFRIANQYGLFAVMTRGRYEIEFQGSNDGENWTPYPFRHKPQALNGMPGIYAPYQPR
                     FDWNLWFASLTDFQNAEIVPLAEERLLEGDGAVLSLFKGDPFHNAPPRYIRAVLWQYW
                     FTTLDEKHDTGNWWRRTLLGLYGPVLTREPGGKFAVVQWPEPLPPHN"
BASE COUNT         1977 a         3175 c         3012 g         1704 t
ORIGIN      
        1 gcgcagctcc aggcggcacg cgcaggtttc gatcttgctg ccgccgccgg gaagggaaat
       61 gacccgcgca ccgcttccgc cacatctggg acaagtaccg ctcatggacg attcagtatc
      121 gcaggttggc cagggtggaa atgccgtccg cattgtggac gacagggcgg gcaggatttt
      181 attcaccggc gctccggatg gtagcgtgag cgtgtggaga gtgattccga gcagatcgtt
      241 gggctctacc agaggcatgc ggcggcctgg gatgagctgc gcagcccggg cagtctcttc
      301 gaaaaaccat ggctcgacaa gtttctcgcc gtggttccgg ccggtggttc ggccctcgat
      361 ctgggctgtg gcggtggcct gcccatctcc ggctacctga tcggccaggg acgcgccgtc
      421 actggggtcg actcctcccc tccattgatc gacctgtgcc gccagcgctt tccgcagcag
      481 gaatggatcg tcgccgacat gcggaccgtt gatctggccc gccggttcga cgctctcatc
      541 gcatgggaca gcttcttcca tctttgcccc aaagatcagc ggcgcatgtt cgtggttttc
      601 tcccgccatg cgaagccagg agcggccctc atgtttacca gcggctcgca tctcggggaa
      661 gcgatcggcg aattccaggg cgagcccctc tatcacggca gcctcgatcc tgaggaatac
      721 gaaaacctgc tggagcagtt tggttttgaa ttggtcgagc atgtcgccga cgaccccggc
      781 tgcggccatc acaccatctg gctcagccgc cgccggggcc tgcaaacgga ctcacgaagg
      841 tcatcttccg gcgtgcagca cgccgatata tgatggggaa tcggatggac tgatttccgc
      901 atccggccca cccacggtct tctcagcatt acgtttagcg aacttaagaa aaacagcgaa
      961 cgtgtgtgcg gccgcgccgg gaatcagaaa ggaaagcagg cgaaccggtg acccaggcga
     1021 agtcggcagt ggtagttggc gcccagtggg gtgatgaggg taaaggcaag atcgtcgatg
     1081 tgctgagcga gcgcttcacc gtggtggcgc gctacgccgg cggccacaat gcaggccaca
     1141 ccgtcatcat cgacggtaag aagttcatcc tgcaacttgt gccctgcggc gtgctgcggc
     1201 ctggatgccg cggcgtgatc ggcaatggcg ttgtcctcga tcccatggcc ttcctgaagg
     1261 aaacaggacg cctgcgggaa ctcggcatcg acgtcgacag ccgcctcttc gtctccaacc
     1321 gcgcccaggt catcctgccg taccaccgca tgatcgagct ggcggcagaa agcgcaccgg
     1381 gacggcagaa aatcggcacg accagccgcg gcattgggcc ggcttatgag gacaagatgg
     1441 cgcgcaacgg gctgcgcgtc gttgacctgc tcaacaccag cctgctcaag acgcacatcg
     1501 agaacgcctg cggcgaaaag aacgccatcg cgcatgccct cttcggcacg gagcctctcg
     1561 atcctgcgca gatgtatgac gagtacactg ccgcggccga gaaaatccgt ccctttgtca
     1621 ccgatacctc cctgctcctg aacgacgcgc tcaaggaagg tcacagcctc atgttcgagg
     1681 gggcgcaggg caccatgctg gatatcgacc acggcactta ccccttcgtg acgtcgtctt
     1741 cttcggcggc ggggggcgcg tcgacaggaa ccggcgtcgg gccgacgcag atcggcaccg
     1801 tcattggcgt caccaaggct tacgtcacgc gggttgggga aggaccgttt cccaccgaga
     1861 ttcacgacga atccggcgaa atgctgcgcg cccggggtca ggaatacggc gcggtcacgg
     1921 ggaggccgcg ccgctgcggc tggctcgacc tgccgctgct gcgctattcg aaccagatca
     1981 acggcaccga atggctggtc atcaccaagc tcgacgtgct cgatgaaatg gactggattc
     2041 ctgtttgtac ggcgtacgag gtggatggca agccctgcga caccatgccg gccgatatcc
     2101 gcggcatcga gtccatccgc cccgtgtaca ctcggctgaa aggctggaag acttccaccg
     2161 aaggcgttcg cagtttcgac gatcttccca gggttgcgca ggagtacctg cgcttcatgg
     2221 aaaaagagac gggggcaaag atcggcatcg tctcgaccgg gccggatcgg gatcagacac
     2281 tggaaatgcc ggaattccgc gaggcgatgc ggcggattac ggggaaatag gctcgacgca
     2341 tccggaatgc ctgaccggaa aaaggttcaa tgatcagctt taccgtacgc atgaaattcc
     2401 gctccgacga ccgggcccgt gtggccgaga tcctgaccgc gctggcgcag gcttctcgcc
     2461 gcgagccggg gtgtgtcgca tacataccgc acaccgtcga cggggatgcc gatacggtgg
     2521 tcatctacga gcaataccgg gaccagcagg gggtggacgc ccaccgggca tcagcccact
     2581 ttcgcgacta tgcagtgggc ggcctgtatc agctgatgct ggaccgccag gtcgaaaacc
     2641 tgacatcgat cgcctgaagc cgatgcagtc atccgccctt ttccagcgtc tgaaccagtt
     2701 agggggcgag ccggacggtg tcggcgggtt cgctccccgc tgcatatgac cttccggcgg
     2761 agcatcgacg cagttcggcg catcttcttt gacgccccgc ggcggcccgg gctaacatcc
     2821 ggtacgacca tgcgccgccg cccggcctcc agcctgtgca ccagtgctct cccgcaagcc
     2881 gtcgcggcac tgatcgtgct ggcgtcgtgc tatccggcct gggcgctgcc tcaccgcgac
     2941 aagggcgaga tccataaggt gatcgaatct cttgaggaac agtggcgcca ggccaccgtg
     3001 aataacaaca tcggcgaaat gaaccggctg ctggccgacg actatgtcgg cattacgtcc
     3061 aacggaacgg tcgaaaacaa gcagcaagcc ctggcccagc ggagcgccgg gactatccgc
     3121 atcagcaaac ttgagatcac cgatacgcat gtgcgcgttt acggcgatac ggcggttgtg
     3181 acctcgcagg cgcaactgga aggcagcaac ggcaccagcg acatcagcgg gcagtatcgc
     3241 tacacgcggg tctataaccg acgtcttggc cagtggaaaa tcgtgagctt tgaggcgagc
     3301 cggatgcacg attccgacgc ccgagccggg aacaaacact gaatttctcg cgctcggcgc
     3361 tgcaacgcgc gaccccgctc gtgcaccatg catcgtatcg acaggcggtt gatatactaa
     3421 attgttgcgt ggctttgccc acgccgagga gaaaaaccca tcgataagcg ttctgcgaag
     3481 cagttcgtcc gtatcaacga tcgtatccgc gcccgcgaag tgcgcgtgat cgatgaaaag
     3541 ggtgagcagc tcggtattct tgcccccttt gaggccctta agattgcccg cgagcgggga
     3601 ctcgatcttg tggagatatc gcccacagcg gtcccgccgg tttgccgcat tcaggactac
     3661 ggcaaattcc tttacgaaaa agaaaagagc gatcgggcgg cgcgtaagcg tcagaaggtg
     3721 atcaccctga aggaggttaa attctccgtc accatcgacg accacgacta tgtcacgcgc
     3781 aaaaaccagg ccgtccgctt cctgcaggag ggcgacaagg tgaaggcctc gctgcgcttt
     3841 aagggccgtc agatggccca tcgcgagctg gggtacgcca tcatcaatcg ccttatccag
     3901 gacattggcg aaaatggcgt ggtcgagttc atgccgcgta tggaaggcaa cacccttcat
     3961 gccatccttg cgccgggaaa gaaggagctg ccgaagaaac ccgcggcccc ccgtccgcag
     4021 gctccgcccc ccggtccccc ggcacaacag gcgcagtaag aaaatcgcca aaaaaattca
     4081 gggctgccaa ttgatggcag cccttgttgt ttgcgcccga gggtccgccc gtccctattc
     4141 ggcccgtaat gcctcgaccg gatccacgct cgccgttcgc cgcgaaggca gatagctggc
     4201 cagcgccgcc gccccgacaa gcgctaaaca cgcgctcgcg taggtcaccg gatccatcgg
     4261 gctcacgtgg aagaggagcg atccgagcag ccgcgttacg cccagcgcca cgccaaaacc
     4321 gcacagcaca ccaatcccgg cgagcagcag gccctgccgc acaaacatcc cggtgatgtc
     4381 gttgcgctgc gcgcccacag cgattcggat gccgatctcc cgcctgcgct gcgaggccga
     4441 gtacgcgatg acgccgtaca gacccaccag gccgagcagg agcgcgatca cggccgcaat
     4501 cccaatcatg accagagtga atgaggtccg ggccagcgac ttcgcataca gaaattcctc
     4561 ggtacgcaca tccgacaccg gcaggttgga gtccaccgac cagatggcac ggcgcaggtc
     4621 gccggccagg ctttcggagc cggcgcgctc gctgcgaaca acgaacgtca cccaccgctg
     4681 aatgttgacc ttgtcacctt cgaagttgtt ccgcagcaac ggccagtatg cgattttggg
     4741 tgcttccttg ctgatgccgt catcataggt atcgccgacc acaccaacga tctcgcgcca
     4801 gtcatccgtc gacgccacgc ggatccgctg ccccagcgcc ttttgcgggg agccccagta
     4861 ctcgcgcgca aaatcctccg agacgattgc caccggcacc ttctgataaa tctcatccca
     4921 ggtgtagtcg cgccctgcga tcatcggaat gcccatggtc cggaaaaacc caggcgttac
     4981 gaaacgaaaa cggcgcagcg gcggcagctg ccctgaggca tagctgtgat cctgcgcgaa
     5041 aacgggatcc atccagtggc cgtcatccag cggaacagaa gtcgagatgc tcacactggt
     5101 cacgcccggc accgccgcca tgttgtgctg catggtctcc tgcatgcgca tgacctggac
     5161 cgggtcagaa acttctgcct ccggaatatc gatgtgcagg gtctgaattc ctgccacatc
     5221 gaagccgggg ttgacgtgat tcagcgcgcg gaatgttcgg atcatcaggc cggagcagat
     5281 cagcagcaca aacgcaaggc cgacctggac gaccacgagg gtgttgcggg tgcgatgccg
     5341 ttcgcgtccc tggctcaggc tgcggccgcc ttcgcgcagt ccggttccag cgctcacgtg
     5401 cccgtaacga aacgccggaa tcgacccaaa cagcaaactg cagagcagcg cgagcagtac
     5461 ggcgaagccg agcaccggca gatcgatacc gatatcgccc agtcgcggca ggccggccgg
     5521 ggccaaagcc gtcagcagat gaaccgcgcc ccaggccagc gccagcccaa gggcggcgcc
     5581 agccactcca atgaccgcgc tctccagcag gaattcctgc atcacacgcc agcggctcgc
     5641 gcccagcgcg gagcagatcg ccagttcctg atgccgcccc tcagcccgca ccagcatgag
     5701 attcgccacg ttggcgcatg cgatcagcag cacgatcccg atactgccca gcaggatcca
     5761 cagcgtctgg cccacatcgc cgatgacatc ccgcatcagc gggcggattt ttggacctat
     5821 gcgggccttc ctgaagagct cgatgctgaa tccttcgggc gcgggaaagc tggtccacac
     5881 catcggcagc atccgcgcaa cgtcggtatt tgcggtctcg atggtctgtc ccggacgcag
     5941 gcgcgcgatg ccttcgtagc tgaactggcc gagtttggtc ttgttgcggt cgaattgcag
     6001 cggcaggtaa accccgggct ggatggtcca gtcgaggaag cggaaattcc tgggcatcac
     6061 gccgatgatc tggcgaatct tgccgtctgc ggtgatggag cggccgatca ccgaacggtc
     6121 gccgccaaaa tgccgctgcc agtagccgta gcccagaacg acggtctgcg gagcacccgg
     6181 cgcgacgtcg gccttgctga acgtgcgccc cagcatcggc gcaatgccca gcaccggaaa
     6241 aatcccgtcg gtcacgtcaa tggcggtaat gcgctcggga ctgccctggc cggtgacgct
     6301 gaccgaatcc ccctgataaa tgccgatgtc ggtgaaggcg cggttctgct cgcggtagat
     6361 gaagtagttc gaaggcgcca tgttgacgtc gtcgaggttg agcccaggcg cggtgtgcca
     6421 cacgccgacg agttgctctg ggtgagggta gggaagcggc ttgagcagga cgccctccac
     6481 cacactgaaa accgcgatcg tggcgccgat tcccagggcc agcgtgatga gggtgaccat
     6541 cgtaaacatc ggcgcgcgca gcaaccgccg cagaacctgt ttcagttgcg tcgtgagttc
     6601 catgggcctt cgcctccccg ccagggcgga cctgtccctg tcggtcaaca gtacgcactt
     6661 tttggagaca gggttccggg agcgcgtccc ggacagggga atctgtgacg aacgggcctg
     6721 gccgctgggg cggctgcgct ccgggcggga tctatgccgg gaaacgcgcg gcgctgcgag
     6781 ccttcgcctt cgccgcttct tcttcgcgat tcttcggcgt ggctcccgtc gtcatggcat
     6841 ggagcagccg cgacgaagcc gcgctgatct cctcgatcgc cgcttcgaaa gcggcctcat
     6901 tggcctgcga tggcttcgtg aagccgctga tcttgcgtac gaactgcagc gaagccgcgc
     6961 gaacctcgtc gtcggtgacg ggaggctcaa agttgaaaag cggtttgatg ttgcggcaca
     7021 tgaaagacct accccgccac gcccgccggc tcccggagcc atccatacag ggggaattgc
     7081 tccgccatct ccagcacctg gccgcgaatc cgcgtcagag cgccggaatc cgtccgatga
     7141 tcgagggcct gcgcgatcca gccgccgatg gtacgcatct cgggttcctt cattccgcgc
     7201 gtcgtcagcg ccggggttcc gatgcgaatg cctgacggct tcaacggcgg attcgtgtcg
     7261 aacggaatcg cattcttgtt caccgtgatt ccggcctcgc ccagcgcggc ctctgcttcg
     7321 ctaccgagca tgcccttcgc gaagacgtcg accagcatca ggtgtgtgtc cgttccgccg
     7381 gagatgatgc gatagccatg actcgccagg gtttccgcca gcaccttcgc gtttgccaca
     7441 atctgcgccg cgtaggtttt gaaactcggc tgcagcgctt cgcggaacgc caccgccttg
     7501 gccgcgatga cgtgcatcag cgggccgccc tgctggccag gaaaaaccgt cttgtcgatt
     7561 gcggcggcca gttcctgccg gcacaggatc atgcccgcac gcggaccgcg cagagtcttg
     7621 tgcgtcgtcg tggtcacaat ctgtgcatgg ggaaccgggg agggatgcac gccgcccgcc
     7681 accagcccgg caaagtgcgc catatccacg aggaagagcg cgccgctttt gtcggcaatc
     7741 tcgcgcatgc gcgcgaaatc gaagaagcgc ggataggcgc tgcctccgcc gatgatcaat
     7801 tttggcttct cgcgcaacgc cgtttgctcc agcaagtcat agtcgatcgt ctcggtgtct
     7861 ttgcgcacgc cataggaaac cacgtgatag agcttgccgg agaaattcag cttgtggccg
     7921 tgggtcaggt gaccgcccat ggctagatcc agccccagga tggtgtcgcc cggctgcacc
     7981 accgccgcat aggccgcagc gttggcctgc gaacccgaat gcggctgcac gttggcatgt
     8041 tcggaaccaa aaatctgctt cgcgcggtcg cgcgccaggt tctccaccac gtcggcgtac
     8101 tcgcagccgc cgtaataccg gcgccccgga tagccctcgg cgtacttatt ggtgaacacc
     8161 gaaccggcgg cctccagcac agcgcggctc acgaagtttt ccgaggcgat catctcgaga
     8221 ccttcatgct ggcgccgaac ctcggcgtca atcgcggcgg agacttccgg gtcgtcaaag
     8281 ggaagcgaac gggacatagc gtctggcatg tttacagggt acagggaaca ggttgcaggg
     8341 tacagatggt cctgactgaa acgagcgcgc cgctaattgt gcggcggaag cggctccggc
     8401 cactgcacca cggcaaattt gccgcccggc tcacgcgtca gcaccggacc gtacaaaccc
     8461 agcagggtcc gccgccacca gttgccggtg tcatgctttt cgtccagcgt cgtaaaccag
     8521 tactgccaca gcaccgcgcg aatgtagcgg ggcggcgcat tgtggaatgg atcgcctttg
     8581 aagagcgaaa gaaccgcgcc gtcgccttcg agcaaacgct cctccgccag cggaacgatt
     8641 tccgcgtttt gaaaatccgt gagcgacgcg aaccacagat tccagtcgaa tcgcggctga
     8701 tacggcgcat agatgcccgg catcccgttg agtgcctgcg gcttgtgccg gaagggatag
     8761 ggggtccagt tctccccatc gttcgatccc tggaactcga tctcgtaccg cccgcgcgtc
     8821 atcacggcaa acaagccgta ctgattggcg atgcgaaacg gctccagcgc ggcaatcgga
     8881 aagaccggca gcggtagttc gggccagggc attcgcagca tctcggtcgt cgtcgcatat
     8941 ccgagccacg tcagaatcac cgccgtcaca gcaaggcgta ccgcatgtac gtgcctccac
     9001 acgtccccag gccgaacctc tcgctcctcc gattcttcat cctgtcccgc agccgccttc
     9061 tttgccgcgc gttccttctc ccactccggg gtggtgatca ccgagagccg ttcctcggcc
     9121 atcgtcaccg attcctgcgg aacgtgagct tccagggcat gccggtagcg ctccggaatc
     9181 cacgacagta aggcgcgatc gtccagcagc aggaacccca ggaacagcac caggtaattc
     9241 aggaaggcgt agttcgcggt gaggatgacg ccaatttccc acggtgtgac gatgcagaag
     9301 caaatcaccc gcacccggcg cggcaggaac agcatccaca cgatggccag ctccatgacc
     9361 agcgtccccg cggcagttcc cacgtggaac cagtgcggaa gatgctgtac gtaccagccg
     9421 acgaaggttg gcagcgggcc attctggtag tactgatcca tcgccgacag gctccgccac
     9481 tgcggatcgc cggagagcag cttgacaatg cccgactcga aatagattcg gaaccactcc
     9541 cactgcagca ggaagaaggc gacgcgcgaa ggtggtgaat caaggcccca gcctggccac
     9601 catccgttgg gcgcgacgaa cagcgcgaga aagcccgctt ccagcaacat tccgtcggac
     9661 tggtatccgg aaaaatcgcc ggcggccgcc acgaacgcca gaaagcagac ccagcagacc
     9721 agaaacgaga cgcgcggcca gacattggcg aacgccagca ccgatgcgat cagcccggcc
     9781 cagaccacaa ccatcatggc gtgcgagctg gaagagaacc agaacagggt cggcgcatac
     9841 cagaagcgca gcatcccggg cgactgcc
//
LOCUS       DASZYN010000552         4637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1184574, whole genome shotgun sequence.
ACCESSION   DASZYN010000552 DASZYN010000000
VERSION     DASZYN010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184574"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VHE33_14300"
     CDS             <1..193
                     /locus_tag="VHE33_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98670.1"
                     /translation="EIDGIAERSVEADTLGSEEAEREQPQRVGEEGVPDEGAGQAEAR
                     GKESGEVAALRETWKKSLP"
     gene            complement(213..1370)
                     /locus_tag="VHE33_14305"
     CDS             complement(213..1370)
                     /locus_tag="VHE33_14305"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT98671.1"
                     /translation="MRPRCSLSSLQSVSGLLSFCRSGIPAILVFVLASPAGLKSQRIH
                     AEFDSLSSRELVPQRGQTTSPLIEKELRLAGDYLIGRGVPKDPVQAAYWYRKAADQGD
                     PGAQNQLGYLYTWGIGVERNQAEAFRWYARAAGSGSQAAKLNLAVLYYRGVGTARDVS
                     LAVQMLHELAEKGDPRAQAYLGIVELAGAVVPRDPRAGEKWLSKAAKAKNPEAEQTMG
                     VLWAFTSDHPRDLTKAVKLFRHAARQGYVPAMHALGILLEQHPEIAPNPPDEAIVMLE
                     RAAQAGSWKSSALLGTFARDGHERPQSFADAFRWFTIAQKQGGAAAERLTNANLDRCR
                     QSLTAAQQNEELAAADAWLALHPHTDVYAFSASGVSNYPIDEVYAARSGAE"
     gene            complement(1721..2272)
                     /locus_tag="VHE33_14310"
     CDS             complement(1721..2272)
                     /locus_tag="VHE33_14310"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HVT98672.1"
                     /translation="MTSTDEEMMAAYLLVVLAVLSRVVPFPGMNFTAVGAGLLYFGAR
                     RPLREMALPLLALMGTDFYLTIFAYHYPWHTSAYLVTWAWYAGAIVLGRILLRKQITV
                     TRVLAAPVLASTSFFLISNYAMWIGSVMYPHTLTGLGMCYAAAMPFYRNDLLSTTLIT
                     AVAFGAPVLLAKMSEHRAQKMAA"
     gene            complement(2364..3413)
                     /locus_tag="VHE33_14315"
     CDS             complement(2364..3413)
                     /locus_tag="VHE33_14315"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HVT98673.1"
                     /translation="MEPVTHFLTGACLGRSGFNRKTAYATLAMTLAAEASDLDVLWGF
                     RGPVAELEHHRGWTHSFIGTPIVALATVAFVWLLYRIRRKEPVIPPRWGWLFFCAWIA
                     AMSHILLDYTVNYGVRPFFPWSGKWFAWSIVFIVDPWLLLALTLALLLPAILALADRE
                     IGARKNRFRGRAWAIAALVFMVLWWGLRNAEHDHALALIHAGDYTREPLMRVAAEPHV
                     TNPFRWRILMETQDYFQTAEVDTIGDRVNTDNYANVIYKPPVTPAVAAAKQSYLGRVY
                     LDWGQWPLVEDVGAIEAPGADPPQPGWHTVEFQDLRFGYRPLGTGTSTPLSGWVVVGP
                     GNEIEGMYMSGHEQK"
     gene            complement(3511..3939)
                     /locus_tag="VHE33_14320"
     CDS             complement(3511..3939)
                     /locus_tag="VHE33_14320"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HVT98674.1"
                     /translation="MQRTASAVWRGGLKDGKGTISSQSGVLKDTQYSFSTRFENGIGT
                     NPEELIAAAHAGCFTMALSAQLGNANLTPESLETTAEVKFEKTDEGFTITAIHLTTRA
                     KVPGANASSFETAANNAKNGCPISRLFKGNTQITLDASLV"
     gene            4158..>4637
                     /locus_tag="VHE33_14325"
     CDS             4158..>4637
                     /locus_tag="VHE33_14325"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HVT98675.1"
                     /translation="MSSWSIGVDLGGTNLRVAAVTHHGEAIDTIYLPTRLSAGPEAVL
                     DDMAGAIRQLIEKHGGNLLGIGVGSPGPLELPLGVLHQPPNLPGFDGLNLRVELERRL
                     DRPVQVDSDANLAAYGEFLLGAGRAYGVDSLLMLTLGTGVGCGIVLEGRIWRGMKGAA
                     "
BASE COUNT          873 a         1342 c         1562 g          860 t
ORIGIN      
        1 cgaaatcgac ggcatcgcgg agcgctctgt cgaagcagac acgctcggca gcgaagaagc
       61 ggagcgcgag cagccgcagc gcgtcggcga agaaggcgtc ccggacgaag gggccggcca
      121 ggcggaagcg cgcggcaaag aaagcggcga agtcgcggcg ttgagggaaa cctggaagaa
      181 gagcctgccg tgaaacttcc gggtgcggcc gttcactctg cgccggatcg tgctgcgtag
      241 acctcgtcga tggggtaatt cgacacaccg gaggcactga aggcgtagac gtccgtgtgc
      301 gggtgcagcg cgagccacgc atcggcggcg gcgagttcct cgttttgctg cgcagcggtt
      361 aaggactggc gacagcggtc gagattcgca tttgtcagcc gttcggcggc agctccgcct
      421 tgtttctgtg caatggtgaa ccagcgaaag gcgtcagcga aactctgcgg cctctcgtgg
      481 ccatcgcgtg cgaaggtgcc aagcagggcg gaggacttcc acgagccggc ctgagcagcg
      541 cgttccagca tcacgatggc ttcatccggc ggattgggtg cgatctcggg atgctgctcg
      601 aggagaatgc cgagggcgtg catcgcgggg acatatccct ggcgcgcggc gtggcggaag
      661 agtttcacgg ctttggtgag atcgcgggga tggtcggaag tgaaggccca gaggacgccc
      721 atggtttgtt ccgcttccgg attctttgcc tttgcggctt tgctgagcca tttttctccg
      781 gcacggggat cgcgggggac gacggcgccg gcgagttcga cgatgccgag ataggcctgc
      841 gcgcgcggat cgcctttctc tgccagctca tgcagcatct gaacggcaag cgatacgtcg
      901 cgggcggtgc caacgcctcg gtagtagagg acggccaggt tcagtttcgc cgcctgcgat
      961 ccggatcccg cagcgcgcgc ataccagcgg aaggcctcgg cctgattgcg ctcgacacca
     1021 atgccccagg tatagaggta gccgagctgg ttttgcgcgc caggatcacc ctggtcggcg
     1081 gccttgcgat accagtacgc cgcctggaca gggtctttcg gcacgccgcg gccgatgagg
     1141 tagtcgcctg cgaggcgaag ctctttctca atgagcggcg atgtggtttg gccgcgctgc
     1201 ggcaccagtt cccgagagct cagactgtcg aattcagcgt ggatcctctg gcttttaagc
     1261 cctgcagggg aggcaagcac gaagaccaga atggcgggaa tgccggagcg gcagaacgat
     1321 agcagaccgg aaacggactg cagcgaagac agcgagcaac gaggtcgcat gggcctgctc
     1381 ctctggcaga cggacctgcg cctgttggcc cgtatgcctg ggagtcgacg ctttgctcag
     1441 cggcgtgaat gggacaacaa agcgagggga ccgaaacgac tcggtttcga cccattcaag
     1501 ttttgatccg tcgtttggaa gagggtgtga ttcgaatcac tccacacgcg gggtgcggaa
     1561 atcgaaagta acgttttgca gtgggatcaa aaatacgaag cgggcggcgc tcaggccaca
     1621 ggattgtcga aatggcggac gccgaaaacg cggacggcca cccgccggtg gccgtctttt
     1681 cgtgagttgt tgactgcgcg gggtcgttcc ctcggcggta tcaggcagcc atcttctgtg
     1741 cgcgatgctc ggacatcttc gcgagcagaa cgggcgctcc gaaggcaacg gccgtgatca
     1801 gcgtggtcga cagcaggtcg ttgcgataga acggcatcgc agctgcatag cacatgccca
     1861 ggccggtcag ggtgtggggg tacatcacac tgccgatcca catggcgtag ttgctgatca
     1921 ggaaaaacga agtagaggcc agcaccggag cggcgaggac gcgcgttacc gtgatctgct
     1981 tccggagcag gatgcggccg agcacgatcg cgcccgcata ccatgcccac gtgaccaggt
     2041 aggcgctggt gtgccagggg tagtggtaag caaaaatcgt cagatagaag tcggtaccca
     2101 tcagggccag gagcggcaaa gccatttccc gcagcggacg ccgcgcgcca aagtagagca
     2161 gccccgcgcc gaccgcggtg aaattcattc cggggaacgg caccacccgg ctcaaaactg
     2221 ccagcaccac caacaaatat gcggccatca tttcctcatc ggtcgaagtc aaaagcctta
     2281 ccccattctg cggacgcgga aatctttggt caaggcggac ttgcggtctc atcaagtgac
     2341 tggttcaccg gatgaacaaa acctcacttc tgctcgtgtc ctgacatata catcccctcg
     2401 atttcgttgc cggggccgac gacgacccat ccgctcagcg gtgtgctggt gcctgtaccg
     2461 agcggccgat agccgaaacg caggtcctgg aactcaaccg tgtgccagcc cggttggggc
     2521 ggatcggcgc ccggggcctc gatggctcct acatcttcga cgagcggcca ttgtccccag
     2581 tccaggtaca cgcggccgag ataggactgt ttcgcggcag cgacggcggg cgtaacggga
     2641 ggtttgtaga tcacgttggc gtagttgtcg gtgttcaccc ggtcgccgat ggtgtcgacc
     2701 tcggccgtct ggaagtagtc ctgggtttcc atcaggatcc gccagcggaa gggattggtg
     2761 acgtggggct cggcggccac gcgcatcagc ggctcgcgcg tgtagtcgcc tgcatggatg
     2821 agggccagcg cgtggtcgtg ttccgcgttg cgcagtcccc accagagaac catgaaaacc
     2881 agcgcggcga tggcccaggc tctgccgcgg aaacggtttt ttcgcgcgcc gatttcgcgg
     2941 tcagcgagcg cgaggatggc gggcagaaga agtgcgaggg tgagcgcgag caacagccag
     3001 ggatcgacga tgaagacgat gctccacgca aaccatttgc cgctccacgg aaagaagggc
     3061 cggaccccgt agttgaccgt gtaatcgagg aggatgtggc tcatggcggc aatccatgcg
     3121 cagaaaaaga gccatcccca gcggggcgga atcaccggtt ctttgcggcg gattcgataa
     3181 agcagccaga cgaaagcgac ggtggcgaga gcgacgatcg gcgtgccgat gaaggagtgg
     3241 gtccagccgc ggtggtgctc gagttccgcg accggcccgc gaaagcccca gagaacgtcc
     3301 aggtccgacg cttcggcagc cagcgtcatg gccagcgttg cgtacgcagt cttgcggttg
     3361 aatccggagc gcccgaggca ggctccggtg aggaaatgcg ttacaggttc catgcgcgct
     3421 ttctggaact cacatccagg gtcgttgtct ttttgcgaaa acagcaagcg ccccggcgaa
     3481 ccgaagcgct tgtcagtttt tgttggacgc ctaaaccagg ctggcgtcga gcgtaatctg
     3541 cgtgttgccc ttgaaaagcc gcgaaatggg gcagccgttc ttggcgttgt tggctgcggt
     3601 ctcgaagctg gaggcattcg cgccgggcac cttggcacgg gtagtgaggt ggatggcggt
     3661 gatggtgaat ccctcgtcgg tcttctcgaa tttgacctcg gcggtggtct cgagcgactc
     3721 gggcgtcagg ttggcattgc ccagctgagc gctgagggcc atggtgaagc atccggcgtg
     3781 ggctgcggca atcagctctt ccggattggt tccgatgccg ttctcaaatc gggtggaaaa
     3841 agaatattgc gtatctttca ggacgccgct ctggctggaa atggtgcctt tgccgtcctt
     3901 cagacctccg cgccagacgg cgctcgccgt acgttgcata cgtctctcct tcgatgtggg
     3961 ctgagatttc cccggactgg ataccagctt ggatgccagc gactttgctc ttccccttag
     4021 gaaaatgcgc gaggcatcgc ccggaccatg atatgcgatt tgcctggcgc tcatgcgcac
     4081 gtgcccggca ccctcctaaa ttctgtgccg acccctccat ccggccgtcg tcgcgtatca
     4141 tgggtcttcc aagtggcatg agttcctggt ccatcggcgt cgatctgggc ggcaccaatc
     4201 tgcgggtcgc ggcggtaacg caccacggcg aagcgatcga cacgatctat cttccgacgc
     4261 gcctgagcgc gggacccgag gccgttctcg acgacatggc gggcgcgatt cgccagctga
     4321 ttgagaagca tggcggcaat ctgctgggca tcggcgtggg cagcccgggc ccgctcgaac
     4381 tgccgctggg cgtactccac cagccgccca atcttcccgg tttcgacgga ctgaacctgc
     4441 gggttgaact ggagcggcgc ctggatcgtc cggtgcaggt cgattcggac gcaaatctcg
     4501 ctgcgtatgg cgagttcctt ctcggtgcag gccgcgcgta cggtgtcgat tctctgctga
     4561 tgctgaccct gggcaccgga gtaggctgcg gcattgtgct cgaaggccgc atctggcgcg
     4621 ggatgaaggg cgcagcc
//
LOCUS       DASZYN010000553         2859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_220631, whole genome shotgun sequence.
ACCESSION   DASZYN010000553 DASZYN010000000
VERSION     DASZYN010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220631"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2659
                     /locus_tag="VHE33_14330"
     CDS             <1..2659
                     /locus_tag="VHE33_14330"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HVT98676.1"
                     /translation="WSMDGDGRGLKQESHQKFFDVESASVWDGKAVYASGGDLWLLDL
                     KTGQEAVIPITLVSDFDQMRQHWVKRPLTFLTDTHLSPDGSRVVFTARGEIYTLPAKA
                     GRIIRVAGSDSGVRYRAARFMPDGKSIVALSTKTGETEFWKFPANGEGAPEQWTNDAK
                     VLRWEGVPSPDGRWLAHDDKDQQLWIYDTKTKQNKRIAQSMNGRYSDLSWSPDSQWLA
                     YVETADNEFDQIRVLNVESGKIEILTSDRYNSENPVWSADGKWLYFLSDRALNTTVGS
                     PWGARQPDPHFNHQQKIYEVALVPGLRSPFLPADELHPETPEKEEKKEEPKKPAESAA
                     GSVKAEAGAAKESGRDEAKSDEKKTPDVKIDFAGIEDRLTEFPVPPANYESLAAAAKR
                     LCWLNATDELRPKLTLQCLSIDNKGDEPDTVMADVKGFEISLDRKKMLVHKGDDFYVL
                     DADVKGSALNDAKAAAKAKIDLAHWEMSTTPRDEFRGIFLDAWRLERDYFYDRHMHGV
                     DWVAIRGRYLPLVDRVSDRDELNDVIAQMVSELSALHTFVNGGDRRKPEDDVNVATLG
                     ARLRRDVGDGGFLVEHIYLHDPDLPDTAPPLARPDSLVRDGEVITSIDGVDLKGVRDE
                     RELLRGKAGVQVMLRVKSAKGESRDVLVKPISEDQDENLRYGEWEYTRRLEVEKASSG
                     KIGYVHLRAMGPNDIDQWTREYYPVFNREGLIIDVRHNQGGNIDSWLLGKLLRQAWFY
                     FQPRIGNPSWNMQKAFRGHIVVLCDQETASDGEAFSEGFKRFRMGALIGMRTWGGEIW
                     LSGSNRQADGGVATAAETGVYGPEGKWLIEGHGVDPDIVVDNLPHATFEGNDAELQKA
                     IEVLTGEIQKDPRPVPQHPPYPDKAFKYQ"
BASE COUNT          625 a          732 c         1000 g          502 t
ORIGIN      
        1 ctggtcgatg gatggggacg gacgcgggct caaacaggag agccatcaga aattcttcga
       61 cgtggagagt gcatcggtct gggacgggaa ggcggtctac gcaagcggtg gggacctatg
      121 gctgctggac ctgaagaccg ggcaggaggc ggttatcccc atcacgctgg tttcggattt
      181 cgaccagatg cggcagcact gggtgaaaag gccgctcaca ttcctgacgg acacgcacct
      241 gtcgccggat ggaagccgcg tggtgttcac ggcacgcgga gaaatctaca cgctgccggc
      301 gaaggcgggc cggatcattc gcgtcgcggg cagcgattcg ggagtgcggt atcgcgcggc
      361 gcgctttatg ccggatggca agagcatcgt ggctctatcg acgaagacgg gcgagacgga
      421 gttctggaag tttccggcga acggcgaggg agcgcccgaa cagtggacga atgacgcgaa
      481 ggtgctgcgc tgggaaggag tgccttctcc cgacggacgc tggctggcgc acgacgataa
      541 ggatcagcaa ctctggatct acgatacgaa gacaaagcag aacaagcgca tcgcgcagtc
      601 gatgaatggg cgatattccg atctgagctg gtctccggac agccagtggc tggcctatgt
      661 agagactgcg gacaacgaat tcgatcagat ccgcgtgctg aatgtggagt cgggaaagat
      721 cgagatcctg acatcagacc ggtacaacag cgagaacccg gtgtggagtg cggatggcaa
      781 gtggctctat ttcctgtcgg accgtgcgtt gaataccacg gtgggatcgc cgtggggagc
      841 gcgacaaccc gatccccatt tcaatcacca gcaaaagatc tacgaagttg ccctggtgcc
      901 gggactgcgg tctccttttt tgccggcgga cgaactgcat ccggagacgc ccgagaaaga
      961 agagaagaaa gaagagccaa agaagccggc cgagtcggct gccggtagcg tgaaagctga
     1021 ggcaggagcg gccaaagaat ccgggaggga cgaggcgaag agcgacgaga agaagacgcc
     1081 ggacgtgaag atcgatttcg cgggcatcga agatcggctc acagaatttc ccgttccacc
     1141 ggccaattac gaaagcctgg cagcggctgc gaagcggctg tgctggctga atgcgaccga
     1201 cgaacttcgg ccgaagctga cgctgcagtg cctgagcatc gataacaagg gcgacgagcc
     1261 cgacacggtg atggcagacg tgaagggctt cgaaatttcg ctcgatcgca agaagatgct
     1321 ggtgcacaag ggcgacgatt tttacgttct cgatgcggac gtgaagggct ccgcgctgaa
     1381 cgacgcgaaa gcggcggcga aggcgaagat cgacctggcg cactgggaga tgtcgacaac
     1441 gccgcgggat gaatttcgcg ggatttttct cgacgcctgg cgcctggagc gggactattt
     1501 ctacgaccgg cacatgcatg gcgtggattg ggttgcgata cgaggacggt atcttccgct
     1561 ggtggatcgc gtgtcggatc gcgatgagct gaacgacgtg attgcgcaga tggtcagcga
     1621 gctgtcggcg ctgcacacgt tcgtgaacgg cggcgatcgc agaaagccgg aggacgacgt
     1681 gaatgtggcc acgctgggcg cgcggctgcg gcgcgatgtg ggggatgggg gctttttggt
     1741 ggaacacatc tacctgcacg atcctgattt gcccgataca gcgcccccgc tggcccggcc
     1801 ggattccctg gtcagggacg gcgaggtcat caccagcatt gacggggtgg atttgaaggg
     1861 tgtgcgcgac gagcgcgaac tgctgcgcgg caaagcggga gtccaggtga tgttgcgggt
     1921 gaagtccgcg aagggcgagt cgcgcgacgt cctggtgaaa ccgatcagcg aggaccagga
     1981 cgagaacctg cgctatgggg agtgggagta cacacggcgg ctcgaggtcg agaaagcgtc
     2041 cagcgggaag atcggatatg tccatctgcg cgccatgggt ccaaacgata ttgatcagtg
     2101 gacgcgcgag tattatcccg tcttcaaccg ggaggggctc atcatcgatg ttcgtcacaa
     2161 ccagggcggc aacatcgatt catggctgct gggcaagctg ctgcgtcagg catggttcta
     2221 cttccagccc cggatcggca atccctcctg gaatatgcag aaggcattcc gcggacacat
     2281 cgtcgtgctt tgcgatcagg agacggcttc cgacggtgag gctttcagcg aaggcttcaa
     2341 gcgtttcagg atgggagcgc tgattggtat gcggacctgg ggcggagaaa tctggctcag
     2401 cgggagcaat cggcaggccg atggtggcgt ggcgacggcg gcggagactg gtgtttacgg
     2461 accggaaggg aagtggctga ttgaagggca cggtgtcgat cccgatatcg tggtggacaa
     2521 tctgccccac gccacgtttg aaggcaacga tgcggagctg cagaaagcca tcgaggttct
     2581 gaccggcgag attcagaagg atccgcgtcc ggtcccgcag catcccccgt atcccgacaa
     2641 ggcattcaag tatcagtgat ccggaacggc acgattggcg aatccttgag gggtaagctt
     2701 catccaagaa gtcaccggag ggcgaatggc ggcattttgc ggatgttgtg gcgctgaaat
     2761 tacggcgaag gatgaggtgt gccgcatctg cgggacgcct cgccacggta tgctgcgcgc
     2821 agatcgaatg acgcccctgg atctcggcga cgaagtgac
//
LOCUS       DASZYN010000554         6123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_731081, whole genome shotgun sequence.
ACCESSION   DASZYN010000554 DASZYN010000000
VERSION     DASZYN010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731081"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VHE33_14335"
     CDS             complement(<1..379)
                     /locus_tag="VHE33_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98677.1"
                     /translation="MHGVQISDGGSVQAIAEERWGQKGWELNLAALCSDDAARTVRFL
                     TGAVMACGGYVLSRRFDAEQTAAIEFEFIRGTCVEMYSVLIAAGLELTAQSHTILSTL
                     CQCTRETLEMTASDPVRVDLVIRR"
     gene            437..814
                     /gene="acpS"
                     /locus_tag="VHE33_14340"
     CDS             437..814
                     /gene="acpS"
                     /locus_tag="VHE33_14340"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HVT98678.1"
                     /translation="MLVGTGIDLIEVSRIARSIERYGDRFLERVFTTDEIAYCRAKAS
                     FAESFAARFAAKEAGAKALGTGIQHGVTWKELEVRRAPGSRPTLVLTGRARAIAGQLG
                     VTRVSLSITHTAALAMATVHLEA"
     gene            962..3766
                     /locus_tag="VHE33_14345"
     CDS             962..3766
                     /locus_tag="VHE33_14345"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HVT98679.1"
                     /translation="MAPDDFATDARHQETAGIPAQPTASLDPAPDRVAAGSVIGSYQL
                     LERIGEGGMGEVWLAEQKEPVRRRVAIKLIKLGMDTREVVARFESERQALALMSHPSI
                     TKVFDAGTTPDGRPFFVMEFVAGLPITLYCDRHSLSIRQRLRLFMAVCEGVQHAHQKA
                     IIHRDLKPSNILVTEVDGKPVPRIIDFGVAKATAQRLHATTLHTHAGAPIGTLGYISP
                     EQAGSDGADIDTRSDVYSLGVVLYELLCGALPFDFRKLAWDEVLRALREQDAPGPGAR
                     LHAQGGDSPVTAKNRGTDPSTLFRLLRGDLDAIALKALEKERSRRYASPLDLSSDIGR
                     YLRNEPVSARAPGALYRAHKFVRRHRLIVSMASLLAALVVVFAVAQTVELRRIRRERD
                     RADRITEFLTNMFKVPAPSEARGNTVTAREILDRSSDEIERGLDKDVEVRSQLMLVMA
                     KTYDNLGLFSRAHTLMDHVVQDRRRTLGPSHPKTLEANAQLGWILYREGRDADAERLL
                     RSTIDAQTRVLGADHPSTLESKDHLARVLLRQAHFAEAEFLEQNLIAASTRTQGAESP
                     QTLRFMDGLAIAFTRQSRFAEAESEYRRLLDLERRNLGADHPDTLETMHHLATMYLSQ
                     GNYGEAETLYRDTLQIDQRVLGPEHPDTANVMTTLANTIRFDPRRFAEAENLYRKSLA
                     IELRALGPDHPYTTRTEEGLANVLSAEDRYTEAEGLLRQILSTRQRLLGIEHTDTLIS
                     QYNLASVLRHERRYGDAETLMRDTLAAQTRVLDANDADLFASRTLLANILLDQHKPAE
                     AEPLARQAFIDQLRILGPHHPDTVESLRALGTALVANGRYQEIEKIYLDLIEKMNAGR
                     QEPGDSYLTQAWYDLACFASRSAHRNEAFAYLNHAVDAGYIDVRSMSADPDLKPLQPD
                     PRFNQLVAKARIGRSPLQPQ"
     gene            complement(4177..>6123)
                     /locus_tag="VHE33_14350"
     CDS             complement(4177..>6123)
                     /locus_tag="VHE33_14350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HVT98680.1"
                     /translation="RFGSDASIVGRSVRYGRHRSTVIGVLPADFRLIFPDDASVPANV
                     EVFESIPVGPWQADGPAFLHVIGRLRAGATLAAAQSEMSSAAAQINKLGGRAALGNFT
                     VSVFPLQADTVREVRPTLLCLFAGVGLVLLIGCANIANLLMARARRRLRETTIRAALG
                     ASRGRLARQFLTESVLLGMLGGAAALGTGWAAIRAIVAARPPSFVNFDHVPLDGRVLA
                     FTFAVALLTSMLFGLAPIFSVRRLDLTRDLKESSRQSAGARRPWTSVLVSAEVALAFV
                     LLMGTGLLMRTFINILHTDPGFRADKVLTFRVSVPGYAPLRLIQQKLAALPGVESASA
                     ISHLPLDDAGNWYDAYYKEGASLAEQNSNLADHRSILPGYFRAIGATLLEGRDFTEAD
                     DAQHQHVAILDEVLAQQLWPGQSAIGKRINLSDSPQGFYQFERDWAVVVGVVHHVQYH
                     SLTAMVRPQIYVPYPLAPRPSMSFVLHTPGGGAGLAAAVRSTVDSVNKDIPTTHVEPM
                     HVLVDRAHSDSRFASLLATLLSGIALVLAASGIYGVLSYSVAQRTMEIGIRMAIGAPR
                     GQVLRMVLADGFAWVLPGLIAGFLLSLLVAPLLARLLYGVQPTNAANYAMMLAGVLAV
                     SAVAALLPARRAMKIDPLTALRYE"
BASE COUNT         1233 a         2294 c         1562 g         1034 t
ORIGIN      
        1 ccctccgaat caccagatcc acccgcaccg gatcgcttgc cgtcatctcc agcgtctccc
       61 gcgtgcactg acacaacgtc gaaaggatcg tgtgcgactg cgcggtcagc tccagccccg
      121 cggcgatcag cacgctgtac atctccacgc acgtcccgcg gatgaactcg aactcaatcg
      181 ccgccgtctg ctctgcatcg aaccgccgcg acagcacata accaccgcat gccatcaccg
      241 ccccggtcag aaaccgcacc gtcctggctg cgtcgtccga gcacagcgca gccaggttca
      301 gttcccagcc cttttgcccc caccgctctt ctgcgattgc ctgcacactc cctccatcgg
      361 aaatctgcac gccgtgcatg aacccgattt tccctgccag ggagctaaag atttactcct
      421 gtgctaccgt tgcctcatgt tggtcggcac cggcatcgac ctcattgaag tctctcgcat
      481 cgcacgctcc attgagcgct acggcgatcg cttcctcgaa cgcgtcttta ccaccgacga
      541 aatcgcgtat tgccgcgcca aagccagctt tgctgagagt tttgccgccc gttttgccgc
      601 caaagaagct ggagccaaag ccctcggtac cggcatccag catggcgtta cctggaagga
      661 actcgaagtc cggcgcgctc ccggcagccg ccccactttg gtcctcaccg gtcgcgcacg
      721 tgctatcgcc ggacaactcg gcgtcacgcg tgtctcactt tcaatcacgc acaccgccgc
      781 gctcgccatg gcaaccgtcc acctcgaagc ctgagcgccg ccttcatcct cccgcggcgc
      841 cgtcggtcca tcgctccgag tgtctaatat caaccatgag gctgttgcgc caccgtcacg
      901 tcgtgtgaac agggacacct gctgaggccc caggaccacc ggttcgcaag gagacaccgc
      961 aatggcgcca gatgatttcg caaccgacgc caggcaccag gaaaccgctg gcattccggc
     1021 ccagccaacc gcctccctcg atcccgcacc cgaccgcgtc gctgccggct ccgtcatcgg
     1081 ttcctaccag ctcctcgaac gcatcggcga agggggcatg ggcgaggtct ggctcgccga
     1141 gcaaaaggag cccgtccgcc gtcgcgtcgc catcaagctg atcaagctcg gtatggatac
     1201 acgcgaagtc gtcgcccgct tcgagtccga acgccaggcg ctcgccctga tgtcccatcc
     1261 ctccatcact aaagtcttcg acgccggcac cacgcccgat ggccgcccgt tcttcgtcat
     1321 ggaattcgtt gccggcctgc ccatcactct ctattgcgac cggcacagcc tcagcatccg
     1381 tcagcgcctt cgcctcttca tggccgtctg cgaaggcgtc cagcacgcgc atcagaaagc
     1441 catcatccac cgcgacctca aaccctccaa catcctcgtc accgaggtcg acggcaagcc
     1501 cgtaccccgc atcatcgact tcggcgtcgc caaagccacc gctcagcgcc tccatgccac
     1561 cactctccat acccatgccg gcgctcccat cggtacgctc ggatacatca gccccgaaca
     1621 ggccggctcc gacggagcgg atatcgatac tcgcagcgat gtctattccc tcggcgtcgt
     1681 gctgtacgaa cttctctgcg gtgctctgcc cttcgacttt cgcaagctcg cctgggatga
     1741 agtgctgcgc gctctgcgcg agcaggatgc tcccggtccc ggcgcaaggc tgcacgccca
     1801 gggcggcgac tccccggtca ctgcgaaaaa ccgcggcacc gacccgtcaa ccctcttccg
     1861 cctgcttcgc ggagacctcg acgccatcgc tctcaaggcc ctcgaaaaag agcgctcccg
     1921 ccgctatgca tctcctctcg acctctcctc cgacatcggc cgatacttgc gcaacgagcc
     1981 ggtctcggcg cgcgcgcccg gcgctcttta ccgtgctcat aaatttgtcc gccgccaccg
     2041 cctcatcgtg tccatggcca gcctcctggc ggccctcgtc gtcgtctttg ccgttgctca
     2101 aaccgtcgag ctgcgccgca tccgccgcga acgcgaccgt gccgaccgta tcaccgagtt
     2161 cctcaccaat atgtttaagg tcccggctcc cagcgaagcc cgcggcaaca ccgtcaccgc
     2221 ccgcgaaatt ctcgaccgct cctcggacga aatcgagcgt ggtctcgaca aggatgtcga
     2281 ggtccgatcc cagctcatgc tcgtcatggc caaaacctac gacaacctcg gcctcttttc
     2341 tcgcgcccac accctgatgg accatgtcgt ccaggatcgc cgccgcaccc tcggccccag
     2401 ccatcccaaa actctggaag ccaacgccca gttgggatgg atcctctatc gcgagggacg
     2461 cgatgccgat gccgagcgcc tcctgcgctc cacgatcgac gcacaaaccc gcgtgctcgg
     2521 cgccgaccat ccttccacgc tcgaatccaa agaccatctg gcgagggttc tcctccgcca
     2581 ggcccacttt gccgaagccg agttcctgga acagaacctc atcgccgcca gcacccgcac
     2641 ccagggagcg gaaagccccc aaactctgcg tttcatggat ggcctcgcta tcgccttcac
     2701 gcgccagagc cgatttgccg aagccgaaag tgagtatcgc aggctgctcg atcttgaacg
     2761 ccgcaacctc ggtgccgatc atcccgacac gctcgaaacc atgcatcacc tcgccaccat
     2821 gtatctcagc cagggaaact atggcgaggc cgaaacgttg tatcgcgaca cgctacagat
     2881 cgatcagcgc gtcctcggcc ccgaacatcc cgacaccgcg aacgtcatga ccaccctggc
     2941 caacaccatc cgcttcgatc cgcgccgctt tgccgaagcc gagaacctct accgcaagtc
     3001 cctcgccatc gaactccgcg ccctcgggcc cgatcatccc tacacgacgc gaaccgagga
     3061 aggtctggcc aacgtgcttt ctgccgaaga tcgctacaca gaagcagaag gactcctccg
     3121 tcagatcctc agcacccgcc agcgtcttct cgggatcgaa cataccgaca ctctcatctc
     3181 gcagtacaac ctcgccagcg tcctcagaca cgaaagacgc tatggcgatg ccgaaacgct
     3241 catgcgagac acgctcgcgg cccagacccg tgtcctcgat gccaacgacg ccgacctctt
     3301 tgcatccaga acgctcctcg caaacatcct gcttgatcag cacaaaccag cagaggcgga
     3361 gccattggcg cgccaggcct ttatcgatca gttgcgcatc ctcggtcctc accatcccga
     3421 taccgtcgag agcctcaggg ctctcggcac cgctctggtc gcaaacggtc gctatcagga
     3481 aatagaaaag atctacctgg atctgatcga aaaaatgaac gccggccgcc aggagccggg
     3541 cgatagctac ctcacgcagg cctggtacga cctcgcctgc ttcgcatccc gctctgccca
     3601 ccgcaatgag gcgttcgcgt atctcaacca cgccgtggat gccggttaca tagacgtccg
     3661 ctccatgagc gcagaccccg acctgaagcc cctgcaaccc gatccccgct ttaaccagct
     3721 tgtcgccaaa gcaagaatcg gacgttctcc tctgcagccg cagtgaatag aatcatcggg
     3781 cgcgccgcgc ggcatcaggt tgaggcagct gttcgcaatc ccgacaagat tttcatctcg
     3841 ctgcggagcg cccgcgatcc cagcaatccg tagcgcacaa ctttctctgt atatgggagc
     3901 tgcgaagcag ctcgacctct gtgctttggg tgccgtacag gggcgcgacc tctgtatttg
     3961 ggtgctgcaa aaagcgtgac atctgtattt gggtactgcg cctgcacgac ccctgatttg
     4021 ggtgctgcga accagcctga cctctgtatt tgggcgctgt aaaaaatcgc gaactctttg
     4081 ttttgagtgc tgcgaagcag cacgacggta gaagccccgg ccttcaggcc ggggaaagca
     4141 gcgaccaaac aagatgggct ttagccccgg ccaatgtcac tcataccgca gcgccgtcag
     4201 cggatcgatc ttcatcgccc gccgtgcagg cagcagtgcc gccaccgcac ttacagccag
     4261 cactccagcc agcatcatcg cgtagttggc ggcgttcgtc ggttgcacgc cgtaaagcag
     4321 ccgggcgagc agcggcgcaa ccagcagcga caaaaggaat ccggcaatca accccggcag
     4381 cacccacgcg aacccatcgg caaggaccat ccgcagcacc tgcccacgcg gtgccccaat
     4441 cgccatccga atgccgatct ccatcgtccg ctgcgcaacc gaatacgaca gcaccccata
     4501 gatgccgctc gccgccagca ccagcgcaat tccggaaagc agcgtggcca gcagcgatgc
     4561 aaaccggctg tcggagtgcg cgcgatccac cagcacatgc atcggctcca catgggtcgt
     4621 cggaatgtct ttgttaacgc tgtccaccgt gctccgaacc gccgcagcca gtcccgctcc
     4681 tccacccggc gtatgcagca cgaacgacat cgacggccgc ggcgccagtg gatacggaac
     4741 atagatctgc ggccgcacca tcgctgtcag cgagtggtac tgcacatggt gcaccacgcc
     4801 caccaccacc gcccagtccc gctcaaactg atagaagccc tgcggcgagt ccgacaaatt
     4861 aatcctcttt ccgatcgcgc tctgcccagg ccaaagctgc tgcgccagca cctcgtcgag
     4921 aatcgccaca tgctgatgct gcgcgtcgtc cgcctccgta aagtcgcgcc cctccagcag
     4981 agtcgcgcca atcgccctga agtatcccgg cagaatcgaa cgatggtcgg ccagattcga
     5041 gttctgctcc gccagcgacg cgccttcctt gtaataagcg tcgtaccagt tccccgcatc
     5101 gtccagcgga aggtgcgaga tcgccgaagc cgattccacc cccggcagcg ccgccagctt
     5161 ttgctggatc aggcgcagcg gtgcatagcc cggcaccgac acgcgaaatg tgagaacctt
     5221 atccgcccga aagcctggat ccgtatgcag aatattgatg aacgtccgca tcagcagacc
     5281 cgtgcccatc agcagcacga acgccagcgc cacttccgcc gagacaagaa cgctggtcca
     5341 cggccgtcgc gcccccgccg attgccggct cgactccttt aaatcgcggg tcagatcgag
     5401 ccttcgcacc gagaaaatcg gtgccaggcc aaacagcatg ctggtcagca acgcaaccgc
     5461 gaacgtaaac gcaagcacgc gcccgtccag tggcacatga tcgaagttca caaacgatgg
     5521 cggccgcgca gcgacaatcg cgcggatcgc cgcccacccg gtgcccagcg ccgccgctcc
     5581 gcccagcatc ccaagcagca cgctttccgt caggaactgc cgcgccagcc gcccccgcga
     5641 cgctcccagc gccgctcgaa tcgtcgtctc ccgcagtcgc cgccgcgcgc gcgccatcag
     5701 caggttcgca atgttcgcgc acccgatcag cagcaccagc ccgacccccg caaacaaaca
     5761 cagcagcgtc ggccgcacct cacgcaccgt atcggcctga agagggaaga ccgacaccgt
     5821 gaaattgccc agcgcagcgc gccctccgag tttgttaatt tgcgccgccg ccgagctcat
     5881 ctccgattgc gccgctgcca gtgtcgcccc cgcgcgcagc cgtccaatca catgcaggaa
     5941 cgccggccca tccgcctgcc atggtccaac cggaatcgac tcgaacacct caacgttggc
     6001 cggcacgctc gcatcgtccg gaaaaatcaa ccgaaaatct gccggcagca caccaatcac
     6061 cgtcgaccgg tgccgcccgt aacgcaccga ccgtccgaca atcgacgcat ccgacccgaa
     6121 ccg
//
LOCUS       DASZYN010000555         1819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_346609, whole genome shotgun sequence.
ACCESSION   DASZYN010000555 DASZYN010000000
VERSION     DASZYN010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346609"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VHE33_14355"
     CDS             complement(<1..1011)
                     /locus_tag="VHE33_14355"
                     /inference="COORDINATES: protein motif:HMM:NF036965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVT98681.1"
                     /translation="MAVQLTINGQSAAAEGGLSLFDQAEALGIEVPTSCRKQGKCKEC
                     MVEVVEGAELLSPMTEAERHLKGKFRLSCQTYVTGESGHVRCHTMRRGQMRIERHAVG
                     LPVSGRAMELDPAVTRDGDRILIDGVEVDRCGGPGVSAIHGLAMDLGTTTVVVRLLNL
                     ETGELIADTSFENPQRFGGAEVMSRIAYDTDHPGRLLMRTLAGYLSHAIEKFPVDPKT
                     IYEMVVVGNSTMRDLFFRQNVYSIGQTPYRSITEIEMEEGKRSTTSLEQSGLRSLLPI
                     HPKARVWGAPIISGHVGADAAACMLAVDLAHETRRVAIMDIGTNTELIVGNKDRILAA
                     SCP"
     gene            complement(1139..1402)
                     /locus_tag="VHE33_14360"
     CDS             complement(1139..1402)
                     /locus_tag="VHE33_14360"
                     /inference="COORDINATES: protein motif:HMM:NF025142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HVT98682.1"
                     /translation="MAIYKVLYWQEIPTQVKAEDEEEDVSLMLDGKFMQQVDILAAKR
                     GLQDADDYLAQWKWSEEEEREGSAREVAEAVKAELEAKGWKDV"
     gene            complement(1412..>1819)
                     /locus_tag="VHE33_14365"
     CDS             complement(1412..>1819)
                     /locus_tag="VHE33_14365"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HVT98683.1"
                     /translation="DSTTRPKGVLLMGTVRGDLHDIGKNLVSMMAEGAGFEVHDIGVD
                     QSLEKFMAAADRVKPTIIGMSALLTTTMMYMKTVIDGFEAAGRTEIKMAVGGAPISQM
                     FADEIGADGYGANASAAVDLFLRLAEQTAAATT"
BASE COUNT          315 a          694 c          436 g          374 t
ORIGIN      
        1 cgggcacgac gccgccagaa tccgatcttt gttcccgaca atcagctccg tattcgtgcc
       61 gatatccata atcgcgaccc gccgcgtctc atgtgccaga tccaccgcca gcatgcacgc
      121 cgccgcatcc gcgcccacat gcccgctgat aatcggcgcc ccccacaccc gcgccttcgg
      181 atgaatcggc agcagactcc gcagtccgct ctgctccaga ctcgtcgtcg accgcttgcc
      241 ttcctccatc tcgatttccg tgatcgaccg gtacggcgtc tgcccaatcg agtacacgtt
      301 ctgccgaaag aacaaatccc gcatcgtgga gttgccgacc acaaccatct cgtaaatcgt
      361 ctttgggtcg acgggaaact tctcgatcgc atggctcaga tatcccgcca gcgttcgcat
      421 cagcagtctg cctggatgat cggtatcgta ggcaatccgc gacatcacct cggccccgcc
      481 gaatcgctgc ggattctcaa aagacgtatc ggcgatcagt tcgcccgttt ccagattcaa
      541 gagccgtacc accaccgtcg tcgtgcccag atccatcgca aggccgtgga ttgcgcttac
      601 gcccggaccg ccgcagcgat ccacttccac accatcgatc agaatccggt cgccatcgcg
      661 tgtcaccgcc ggatccagct ccatcgcccg gccgctcacc ggcaacccca cagcatgccg
      721 ctcgatgcgc atctgccccc gtcgcatcgt gtggcagcgc acatgcccgc tctcgcccgt
      781 aacgtacgtc tgacacgaaa gccggaactt ccccttcagg tgccgctccg cctccgtcat
      841 cggcgaaagc agctcggcgc cttccaccac ctccaccatg cattctttgc acttgccctg
      901 cttgcggcac gacgtcggca cctcgatccc gagcgcctcc gcctgatcga acagcgagag
      961 cccgccctct gccgcggccg attgtccgtt gatggtgagc tgcaccgcca tttcgttcag
     1021 gaacctctgc tggacatgcg cgttgaaagg tcacggcttc agccgtggcg taaagccgtg
     1081 aagaccggtc cggcttgagc cgctgaggga agtccttcga ggtctccgga tctgcctctc
     1141 aaacgtcctt ccatcccttc gcttccagct ctgccttcac cgcctccgcc acttcgcgcg
     1201 ccgatccctc gcgctcctct tcctcgctcc acttccactg cgccagataa tcatcggcgt
     1261 cctgcaaccc gcgcttcgcc gccaggatat ccacctgctg cataaacttt ccatccagca
     1321 tcagcgagac gtcctcttcc tcgtcttcag ccttcacctg ggtggggatt tcctgccaat
     1381 agaggacttt atagatggcc atagccgatt cctaagtcgt cgctgccgcg gtctgctccg
     1441 ccaggcgcag aaacagatcg actgccgccg atgcgttcgc cccgtagcca tccgcgccaa
     1501 tctcatcggc gaacatctgg ctgatgggcg cgccccccac cgccatcttg atctccgtcc
     1561 ggcccgccgc ctcgaatcca tcgatgaccg tcttcatgta catcatggtc gtcgtcagca
     1621 gcgcgctcat cccgataatg gtcggcttca ccctgtccgc cgcggccata aacttctcca
     1681 ggctttggtc cacgccgata tcgtgcacct cgaaccccgc gccctccgcc atcatgctca
     1741 ccagattctt cccgatgtca tgcagatcgc cgcgcaccgt gcccatcagc agcacgccct
     1801 tcggccttgt ggtgctgtc
//
LOCUS       DASZYN010000556         3241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_476428, whole genome shotgun sequence.
ACCESSION   DASZYN010000556 DASZYN010000000
VERSION     DASZYN010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476428"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            489..1451
                     /locus_tag="VHE33_14370"
     CDS             489..1451
                     /locus_tag="VHE33_14370"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HVT98684.1"
                     /translation="MRTRFYIIFVAREKDGRLRKIPIPLYYAGVFVAAAVVGAFTITG
                     MAGSYTRMLMKTASFNQVRQQQEALRKDYSQLQREAHEKDIQAASLGSLASEVSALYG
                     LRKSLVAKAAATHVTPGSPDTPAAFTEQTYSQSLDQLALLRASALSGGLRTFSAGMAS
                     NVPADWVNLAGAPSLWPIIGPITSSFGERQDPFNGEGAFHSGVDISGTFGEPVRATAD
                     GIVEVASMASGYGREILVKHSNGIETLYGHLSGFAVTAGSEVRRGQVIGYVGMSGRTT
                     GPHLHYEVRIHNTPVNPYRYLRETVQQLATADAAANAGMSKGGY"
     gene            1506..1901
                     /locus_tag="VHE33_14375"
     CDS             1506..1901
                     /locus_tag="VHE33_14375"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT98685.1"
                     /translation="MLKSITPFFIVDNLQATIDFYESKLGFSVRYKGGGDAESEDYWV
                     FMGRDQVMLMFKAIAPDIHPQSNSSRHEWARWDAYLYTDDPDGLYQAYLGQGVRMHSE
                     LANTSDGLRAFEVKDNSGYVLCFGRLVEK"
     gene            complement(1922..>3241)
                     /locus_tag="VHE33_14380"
     CDS             complement(1922..>3241)
                     /locus_tag="VHE33_14380"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HVT98686.1"
                     /translation="SMMPLRPLALVALTLSIPLSSRPQPTAKMAHKRAPQTAAAVHRS
                     AIVIDTHADTPQRMLDDHYDLTEPLDGGYLNFESAKQGNLGAEFFSIWVEPKLNEGHY
                     ARRTLELIDAVHQQALRHPDKMMMAYSPEDILRAHREHKLAALMGIEGGHSIEDSLGL
                     LRDYYRLGVRYMTLTWSNSNSWADSSGDADDPNVHHTEDGLSDFGKDVVYEMNRLGMM
                     VDISHVSDKTFYRAVITSRAPVFASHSAARALTNVPRNMTDDMLRAVARSGGPNSKGG
                     VVMVNFYSGFISDAWWQAWRAQAAERKAGEDAQIAQWKAEGKTITYEMTDALDKQWAS
                     KIPRPPLSALIDHIDHVAKVAGIDHVGLGSDFDGIPSSPEGMDSAADLPKITAALMER
                     GYSPEDCRKILGANFLRFFREVQATAKELQAQSAERPAITNQQPFKK"
BASE COUNT          635 a          913 c         1066 g          627 t
ORIGIN      
        1 gcggtttggc gcagaaagat ggtggaaaac cccggtacta aggtaacgaa ggccggagaa
       61 accgcttgca tcgccggatg ccccgggtaa actattggaa agggatcggt agacgatccc
      121 tttttcattg ggggcgtctc cgtagttggc tcttgcggtc tttcgccggc gagaggggta
      181 aaccaacggg ggatgccgtt acgacagagg gcaagaccga aacgggaacc gggctacgtt
      241 gtccggtttg ttaccattga taagtcacgg atttcaaagg gcggagtgca gcaagggaaa
      301 aacgctctgc acaattcctg caacgtacga ttacctgagt acttgatccc acgaatggtc
      361 ggttgacctg caatcaggct actgttaact ttcaagccac aaaaacaaaa cagtctgtac
      421 ccgctgtctt gccctggctt cgaaagatcg attgaggggt tctccgagag tatccggaaa
      481 aactggcatt gcgcacgcgt ttctacatca ttttcgttgc acgcgagaag gacgggcgtc
      541 tccgcaagat cccgatcccc ctttactacg ccggagtgtt tgttgccgcg gcagtagtgg
      601 gggcgttcac gatcaccggg atggccggct cctacacgcg catgctgatg aagaccgcca
      661 gcttcaatca ggtgcggcaa cagcaggaag cgctgcgcaa ggactacagc cagctgcagc
      721 gtgaggccca cgagaaagac atccaggcgg ctagcctggg ttcgctggcc agcgaagtaa
      781 gcgcactgta cggtctgcgc aagagcctgg tggcaaaagc cgcggccacc catgtcacgc
      841 ccggaagtcc ggacactccg gcagccttta ccgagcagac ctattcccag tctctggatc
      901 agctggctct gctccgggcg agcgcgctgt cgggcgggct gcggaccttc agcgcgggaa
      961 tggcatcgaa tgtcccggcc gactgggtga atctggcggg agcgccgtcc ctgtggccga
     1021 tcatagggcc catcacaagc tcctttgggg agcgtcagga cccgttcaat ggggaaggcg
     1081 ctttccattc gggcgtcgac atttccggga cgttcggcga gccggtgcgt gcgaccgcgg
     1141 atggcatagt tgaagtggcc tcgatggcca gcggatacgg ccgcgagatt ctggtgaagc
     1201 acagcaacgg gattgagacg ctgtacgggc atttgtccgg gttcgcagtg acagcgggat
     1261 cggaagtgcg ccgcggccag gtgattggct acgtcggcat gagtggccgg acaacggggc
     1321 cgcacctgca ctatgaggtc cgcatccaca acacgccggt gaatccgtac cgctatctgc
     1381 gcgagaccgt gcagcagctg gcgacggccg atgccgctgc caatgccggg atgagcaagg
     1441 gcggctactg acccgagccc cgggtcgcag aacagctggc cgagtgtcag ggtaagctgt
     1501 tcagcatgct gaaaagcatc actccttttt tcatcgtgga caatctgcag gcgacgatcg
     1561 acttctacga gtcgaagctc ggcttcagtg tgcggtacaa gggcggcggc gacgcggagt
     1621 ccgaggatta ctgggtgttc atgggccgcg atcaggtcat gctgatgttc aaggccatcg
     1681 caccagacat tcatcctcag tcgaactctt cacggcacga gtgggcccga tgggacgcct
     1741 atctctacac tgacgatccg gacgggctgt atcaggcata cctgggccag ggagttcgca
     1801 tgcactcgga gttggcgaac acaagcgacg ggttgcgggc gttcgaggtg aaggacaaca
     1861 gcggctatgt gttgtgcttc ggacggctcg tggagaagta gctccgctgg tcgaacgcgg
     1921 cctacttttt gaatggctgc tgattcgtaa tcgccgggcg ctccgcggac tgcgcctgga
     1981 gctctttcgc cgtcgcctgg acttcccgga agaaacgcag gaaattcgct cccagaatct
     2041 tgcggcagtc ttccggcgaa tagccgcgct ccatcagcgc tgccgtgatc ttcggcagat
     2101 cggccgcaga gtccatgcct tcgggggagg aagggatgcc gtcgaaatcc gacccgaggc
     2161 cgacgtggtc gatgccggcg acttttgcaa cgtggtcgat gtgatcgatc agcgcgctga
     2221 gcggcgggcg gggaatcttc gatgcccact gcttgtcaag cgcgtcggtc atctcgtacg
     2281 tgatggtctt gccttcggcc ttccactgcg cgatctgcgc gtcttcaccg gccttccgct
     2341 ccgcagcctg cgccctccag gcttgccacc aggcgtcgga gatgaatcca gaatagaaat
     2401 tcaccatcac cacgccgccc ttgctgttgg ggccgccgct gcgggcgaca gcgcgcagca
     2461 tgtcgtcggt catattgcgc gggacgttgg tgagcgcgcg cgctgcggag tgcgaagcga
     2521 aaacgggcgc gcgtgaagtg atgacggcgc ggtagaaagt cttgtcggaa acgtgggaga
     2581 tgtcaaccat catccccagg cggttcatct cgtagacgac gtccttacca aaatccgaca
     2641 ggccatcctc ggtatggtgg acgttcgggt cgtcggcgtc gcccgaggag tcggcccagc
     2701 tgtttgagtt cgaccacgta agcgtcatgt agcggacgcc gagacggtag tagtcgcgca
     2761 gcaggccgag ggagtcctcg atggagtggc cgccctcaat gcccatcagc gcggcgagct
     2821 tgtgctcgcg atgggcgcgc aggatgtcct cgggggaata cgccatcatc atcttgtcgg
     2881 gatgccgcag cgcctgctga tggacagcgt caatcaattc cagcgtccgc cgcgcatagt
     2941 ggccctcgtt cagcttgggc tcaacccaga tggaaaagaa ctcagcgccg aggttcccct
     3001 gcttcgcgga ctcaaagtta agatacccgc cgtcgagagg ctcggtgagg tcgtagtggt
     3061 catccagcat gcgctgaggc gtgtcggcgt gcgtgtcgat gacgatggcg gagcgatgaa
     3121 cggcggctgc ggtctgggga gcgcgtttgt gggccatttt tgcggtgggc tgcggtctgg
     3181 aggaaagtgg aatcgacaaa gtcagggcaa cgagggcgag tggccgcaga ggcatcatgc
     3241 t
//
LOCUS       DASZYN010000557        11226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_346780, whole genome shotgun sequence.
ACCESSION   DASZYN010000557 DASZYN010000000
VERSION     DASZYN010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 11226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11226
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346780"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VHE33_14385"
     CDS             <1..309
                     /locus_tag="VHE33_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98687.1"
                     /translation="ALDPVTQSQIVDLLRNLNRRHSMTLLYISHDLTSVIRLCDRVAV
                     LHEGSLVENVPVGQLNEVRHPASLSLLRALPAPPEMLLRFRDDTAAEELLQTPDYLRK
                     "
     gene            479..2353
                     /locus_tag="VHE33_14390"
     CDS             479..2353
                     /locus_tag="VHE33_14390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HVT98688.1"
                     /translation="MFRSLFRLPVVLGTLVAVSATSFAAKAPASNIKISPDLASSPSS
                     ASQPVIIQYYNAPSPLETGLLGLVGGLLKGVLGTINAVVADVTPARLNLIAADPNVRY
                     ISPDRSLGARGVGNITAAEFTSEPINAPAVWQQGYKGTNIGVAVIDSGITPVADLGQN
                     PGLPSLNSVSYLLASLGQVAPGATGRIVYSQNFVANQHDALDHFGHGTHVAGLIAGNG
                     SQSSGRQYFRSFIGSAPNANLINLRVLDENGAGTDSAVIAAIERAIALKNKYNIRVIN
                     LSLGRPIYESYKLDPLCQAVEQAWKAGIVVVVAAGNDGRDLNLNPEGYGTIDAPGNDP
                     YALTVGAMNTRETASVQDDIIASYSSKGPSFIDHVVKPDVVAPGNLLSSLQYSNDSLA
                     QNNPSFYTLYSFYRNKGSETPSADYFPLSGTSMATGVTSGAIADLLQASPQLTPDQVK
                     ALVMVNADRSYFPVSSSVTDEGIEYKANYDVFTIGAGYLDIAATVNAAVSHRQHVPAG
                     TAMSPVAVYDEATGNTTVVIDDSSLWSSTGLWSAGSVYGSRAFITSGSGSDAVWGSTA
                     LWGRSDPDGYTALWGRTALWGRDTPEAETALWGRNSTEGQTALWGRSDPGDTNVSVQF
                     "
     gene            complement(2431..3891)
                     /locus_tag="VHE33_14395"
     CDS             complement(2431..3891)
                     /locus_tag="VHE33_14395"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HVT98689.1"
                     /translation="MPGISLKAVAGPRSTSHAALQAILIAVGGDLAIADSQGRALVGQ
                     SSPANSKTSVPILHEGAQLGVVAGDEGSARAIALLLSQMAAREAEGRAMAAEVLHLYR
                     EIHLIEELSEQLAPLLDGEAIAKAALDQAQRLIAANRGVVLFMDQTGALQTLARIGDE
                     DAETSAIGPRSALVASILERGVAEIVNDQPDSSSRNGQGAGAMLAAPLRAGERTVGVI
                     ALAHSDEGGHYSSADLKLLNTIGLQTATALEKARLFSDMVSSERERAEYAAELKAASA
                     VQQLLLESASRPTPGFQVDSVYLPAKEAGGDFFYVQPSGDGSLLAIVGDVSGKGLTAA
                     MRVAMILGVLSRETSEEPAEILFHLNNALVAQGQLGFTTACCLRIAPGGDFTLANAGH
                     IAPYISGEELETIPALPLGLVPGSTYSEVQGKVPPGERLVLMSDGVPEARSASGELLG
                     FEQLAALTRLTAGEIADAACRFGQEDDITVLTITPL"
     gene            complement(3898..4290)
                     /locus_tag="VHE33_14400"
     CDS             complement(3898..4290)
                     /locus_tag="VHE33_14400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT98690.1"
                     /translation="MTPKILIVDDEPHLRTLIQQALEELEDDGVELLTATNGEEALAT
                     IRAEEPNLVFLDVMMPRLNGFDVCNTVKNQLGLGHIHIVLLTAKGQEFDRQRGQEVGA
                     DLYMTKPFDPDALLAKARSVLGLTAIGD"
     gene            complement(4287..9296)
                     /locus_tag="VHE33_14405"
     CDS             complement(4287..9296)
                     /locus_tag="VHE33_14405"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVT98691.1"
                     /translation="MALRIRPRLWLVALLALSSLLPCAVAGTSSAAEAAPGKPEAGMP
                     VVQFYSVKDYNGGPQVWTIAQDRRGLMYIGASGGALMQYDGVTWRKIFLATSVIRSLA
                     LDNDGTIWLGANGNVGYLAPDATGQLQFVSLRDKIATQDRGFTDVWQALATEHGVFFR
                     SYEELLRWDGTRMQVWKPQQPAQRYQALSEVRGHIYTAQQGIGLEEIVGDELRPVPGG
                     DAYKDSIKLFLYPWDNGQILVSARGKPLTLYDGQKVKPFPTGADDFFHKFPPYTSVIL
                     PDGGIGVTTNGGGMAVLDHDGKLREILDQHSGLPDPDALSAFVDRDKALWVGTEGGVA
                     RVEINSPISIFSTIPIYDVAQFNGNVYTTTTGTTAAAGRLGVDAKTGVPTLIPLQGHP
                     NQGWTLLVFKDPAGKTPDQLLIATSEGVMKLKGDQMVPAMAGVQDLAEQTYALVQSRL
                     TPSRVFIGHSDGVGSMRWDGHAWVDEGRAPGLIYEARNFAEDSEGYLWVSGADGKMLR
                     GHVAPTGMRDSHFEAVGKAEGLPEGSSDAELVGKDLYTTMDRAKHIYRWDYAAHRFVV
                     DDTFLLPVDGPDASAVLSPNPDGSVWSETTSSDNIRLGLFRPGADGKWNVDEDRYRIL
                     LRFRTFLMHNEADGIWVTGERLLRFNPGATASSTQPFQTLIRQVSAGKQTVFGGVQVA
                     DRPTLRLPAGTSAMRFEFAAITFGNPSGTSYQYLLEGADHDWSDWGKQKEANYSGLGP
                     GTYRFRVRSRADDGRVGEEGDFSFTILPPWYRSTLAYVLYALLFLLSTFAIWTAIIRY
                     ERAKARRRTVELEAQARELEATVSARTQEIRAQATEISAQKESIELLSDIGKEITASL
                     DLSTILFRLYERVNQIVDASIFGVGLYRPEKRLIEYSLAIENGIRYAPYTRSTDDKNQ
                     LAVWCIDHRKPILLNDVATESSKYIASYDHGARTLEDGSAAQPPASMIYLPLIAQDRV
                     LGVLSVQSFKKNAYTEQHLSLLENLAAYTTIALDNANAYQTINQREREVSERAAELVT
                     INRITQALASQLDRDRLIQFVGDQVRDVFHAPVVYLALLDRATMMLHFPYTWGEEAPS
                     RPFGTGLTSQIIRTGQPLLINEDLDANRTKLGIEKVGRSTASYLGVPIHSGGQAIGVI
                     SVQSTDQEGRFTEADQRLLSTIASAVGVAFYNARLFDETRQARAAAEEADAAKSSFLS
                     TVSHELRTPLTSVLGFAKIIRRRLQERLFPLITGDDRKVQQAKQQVIENLDVVVSEGE
                     RLTKLIDDVLDLAKIEAGKFTWNLSTVSIADVIERATAATASLFEAKRLHLIRDVDLD
                     LPPITGDQDRLIQVVINLISNAVKFTDSGSIACAAHQRDNELVVSVKDSGIGIAAGDQ
                     SKVFEKFKQVGDTLTDKPKGTGLGLPICKEIVEYHGGRIWVESEPGRGSTFLFTLPIT
                     AKSAQLPLLPERRSLDFESLVRQLRDTVVAQNGRSKSVLVVDDDPNIRSLLQQELTEA
                     GYAVRLAEDGRKALALIREEMPGLVILDVMMPEMNGFDVAAVLKNDPATMDIPIIILS
                     IVEDKERGFRLGVDRYLTKPIDTKSLFHEVDSLLGQGKSKKKVMVVDEDASTIRTLTD
                     VLEARGYQVVDSDGKDLVTRAVTSRPDIIILNSLLSSSDGVRSLRFEKGLENVLFLIY
                     S"
     gene            9526..>11226
                     /locus_tag="VHE33_14410"
     CDS             9526..>11226
                     /locus_tag="VHE33_14410"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HVT98692.1"
                     /translation="MSSPRDPTRREFLTVGSAAALAAAATAVGAQTNALPLDTANLSS
                     ATAGSDDPFHVQSQRTFSGETATQVAMPLGGIGSGCICLNGYGGLQDFSIRTRPETSS
                     LPEGFTSDSPEAGFALLHIKGANSVTKLVEGPFPVFKIFDQGLQGQGLRRGGFEGFPR
                     FAKATFRGQYPFGKVDFNDPLLPLHVTLVGWNPFIPLDDKNSGIPCVILEYSLSNNSA
                     APVEYEFSYHLSHLAPGCQPDQAASLNTVIPGKGVFLHNREAAHAESYGSATLTVIGA
                     TPRIKGMWLRSPGWEFDSLSALWREVSSGKFTANDGSNDVDTAGRNGASLLLEGTLAP
                     GESRTYPIVIAWHFPNCYLQDGGVSVAANVKGSPGCRDVTSAPPWRPWYVTAWSDARD
                     VAVYVEKEYASLRQRTMQFHDALFSSTLPRSVLDAVSANLGILKSPTVLREENGNLWG
                     WEGCFPDSGCCHGSCTHVWNYAQAFPNLYPQLERTLRELELVRSMDEHGHVNFRGAIP
                     DGPVPHDGPAAADGQLGGIMKLYRDWQISGDTAWLNKLYPLAKRSLDYCIHQWDPDHR
                     G"
BASE COUNT         2206 a         3667 c         3149 g         2204 t
ORIGIN      
        1 gcgctcgatc ctgtaaccca gtctcagatc gtcgatctgc tcaggaatct gaatcgccgc
       61 catagcatga cgctcctcta tatctcgcac gatctgacct cagtgatccg tctctgcgac
      121 cgggtcgccg tcctgcacga aggctccctc gtcgagaacg ttcccgttgg acaactcaat
      181 gaggttcgac acccggcctc cctgtctctg ctgcgtgcgc ttccggctcc cccggagatg
      241 ctgctccgct ttcgagacga tactgccgca gaggagttac tacagacacc tgattacctg
      301 cggaaataac ggaagcccgc ccccttacca caacactgcc attgttggtt ttcacgagcg
      361 ctcatacctt acgaagtgtg cagggcatgc atgatcccag gtctccccag agacaaaatg
      421 cagcccacac aggatcccga gagcaagtga actcgatgat cccggagatc gtaagacaat
      481 gttccgttcc ctgtttcgtc tgcctgtggt tctcggtact ctcgtcgccg tttccgccac
      541 cagcttcgcg gcgaaggccc cggcctcgaa tatcaagatc tcgcccgacc tggcgtcgtc
      601 cccatcgtcg gcaagccagc cggtcatcat tcagtactac aacgcgccgt ctcccctcga
      661 gaccggacta ctcggtctgg tgggtggact gctcaaaggc gttcttggaa cgatcaacgc
      721 tgtggtggcc gatgtcaccc ccgcccgcct caatctcatt gccgccgatc cgaacgtccg
      781 ctacatttct ccggatcgct ctcttggcgc gcgtggcgtg ggcaacatta ctgccgctga
      841 gttcaccagc gagcccatca acgcgccggc agtgtggcag cagggctaca aaggcaccaa
      901 catcggcgtc gccgtcatcg acagcggcat caccccggtt gcggaccttg gccagaaccc
      961 tggtcttccc tctctcaact cggtcagcta cctgcttgca tcgctcggtc aggtggctcc
     1021 cggcgcgacg ggacgcatcg tctatagcca gaactttgtt gccaaccagc acgacgccct
     1081 cgaccacttc ggccacggaa cccacgtcgc cggactgatc gccggcaacg gctcgcagtc
     1141 cagcggacgt cagtacttcc gcagctttat cggttcggct ccgaacgcca acctcatcaa
     1201 cctgcgcgtt ctggacgaga acggcgcggg caccgactcc gccgtgattg cggccattga
     1261 gcgtgcgatc gcgctgaaga acaaatacaa catcagagtc atcaatcttt ctctcggccg
     1321 cccgatctac gagagctata agctcgatcc gctttgccag gcagtggaac aggcgtggaa
     1381 ggcaggaatc gttgtggtgg tcgcggcagg taatgatggc cgcgatttga acctgaaccc
     1441 tgagggctat ggaacgatcg atgcccccgg caacgacccg tacgcactga ccgtcggagc
     1501 gatgaacact cgcgaaaccg cctcggttca ggacgacatt atcgccagct acagttcgaa
     1561 ggggccgtct ttcatcgatc acgtagtgaa gccggacgtt gtcgcacccg gcaatctgct
     1621 ctcgagcctg caatattcga acgattcact ggctcagaac aatccttcgt tctacaccct
     1681 gtattccttc taccggaaca aaggctctga gacgccgtct gcggattact tcccgctgag
     1741 tggaaccagc atggcgaccg gcgtcaccag cggcgcaatc gctgacttat tgcaggcttc
     1801 gccgcagcta actccggatc aggtcaaagc tctcgtcatg gtcaatgcgg atcgcagcta
     1861 tttccctgtt tcgagcagcg tgactgatga aggcattgag tacaaggcga actacgatgt
     1921 gttcacgatt ggcgcaggct atctcgatat cgccgccacg gtgaacgcag ccgtgagtca
     1981 tcgtcagcac gtgccggccg gcaccgccat gtctcctgtt gccgtttacg atgaggcaac
     2041 cggaaacacc actgtagtga tcgacgacag ctctctctgg tcctcgacag gactctggtc
     2101 cgccggcagc gtctatggga gccgcgcgtt catcacctcc ggcagcggca gcgatgctgt
     2161 ttggggttcg accgctctgt ggggcaggag cgatcccgac ggctacaccg ctctgtgggg
     2221 acgcaccgcc ctgtggggaa gagatacacc ggaagcagag accgcactct ggggcagaaa
     2281 ttcaaccgaa ggccaaacgg ctttgtgggg aagatccgac cctggagaca ccaacgtctc
     2341 cgtgcaattt tgatttgaag cttgccgctg gctaatctcc cgcatcctga caacaggaac
     2401 ggaaaaccat ccgcaaacgt cgggggattg ctagagcggt gtgatcgtta gcaccgtgat
     2461 gtcgtcttcc tgtccaaacc ggcaggcagc gtcggcaatt tcaccggcag tcagtcgggt
     2521 cagagccgct aactgctcga atccaagcaa ctctcctgac gctgatcgcg cctcgggcac
     2581 gccatccgac ataagaacca gccgctcgcc gggcggaact ttcccttgta cttctgagta
     2641 agtcgagcca ggaactaatc caagtggcaa tgccgggatc gtctccagct cctcaccgga
     2701 gatatacggt gctatgtgcc cggcattcgc tagcgtgaaa tcgccgcccg gcgctatgcg
     2761 caaacagcaa gcggtcgtaa atcccagctg tccctgtgct accagagcat tgttcagatg
     2821 aaaaagaatc tccgcaggct cttccgacgt ctcgcggctc aacacgccaa gaatcatcgc
     2881 cacccgcatc gctgcggtga gccccttgcc cgaaacatcc cccacaatcg cgagcagaga
     2941 tccgtcaccg ctgggctgaa cgtaaaagaa atctccgcca gcctcctttg cggggaggta
     3001 gaccgagtcg acctggaatc caggcgtggg ccgcgaggcg ctttcgagca gcaactgctg
     3061 cacagcgctg gcagccttga gttcggcggc gtactctgcg cgctcgcgct cagagctcac
     3121 catgtccgag aaaagccgag ccttctccag cgccgtcgcc gtctgcagac cgatcgtgtt
     3181 cagcagcttc agatcggccg atgagtaatg cccgccttcg tccgaatgag cgagcgcaat
     3241 gactcctaca gttcgctctc ccgcacgcag gggcgcggcg agcatcgctc ccgctccctg
     3301 accgttgcgt gaggaagagt ccggctgatc gttgacaatc tcagctacac cgcgctccag
     3361 aatggaagcg accagcgcgg accgcggacc gatcgcgctc gtctccgcat cctcatctcc
     3421 gatgcgagcc agagtctgca aagctcccgt ctgatccatg aacagaacca cgccgcggtt
     3481 cgccgcgatc agccgttgcg cctgatccag agccgccttc gcgatcgcct ccccatccag
     3541 cagcggagcc agctgctccg acagttcctc gatgagatga atttcgcgat aaagatgcag
     3601 cacctcggcc gccatggcgc gaccttccgc ctcacgcgca gccatctgcg aaagcagcag
     3661 cgcgatagcg cgcgccgaac cttcatctcc ggctacaact ccgagctgag cgccctcatg
     3721 aagaatcgga accgaagttt tggaattcgc aggcgaactt tggccgacga gcgcccggcc
     3781 ctggctgtcg gcaatcgcca aatcgccgcc cacggcaata aggatcgcct gcagcgccgc
     3841 atggctggtg gatcgcgggc cagccaccgc cttcaaactg atcccgggca tgaaagctta
     3901 atccccgatc gccgtcagac ccagcaccga acgtgccttc gccagcagag catccggatc
     3961 gaaaggcttg gtcatataaa ggtccgcgcc gacttcctgt ccgcgctgac gatcgaactc
     4021 ctgcccctta gccgtcagca ggacgatatg aatgtgcccc agaccaagct gattcttgac
     4081 cgtgttgcac acgtcgaagc cattcagccg cggcatcatc acgtcgagaa aaacaaggtt
     4141 cggctcctcc gcccgaatgg tggccagcgc ctcctcgcca ttggtggcgg tcagcagttc
     4201 caccccgtcg tcttccagct cttccagagc ctgctggatc agcgtgcgca ggtgaggttc
     4261 atcgtcgacg atcagaatct tcggtgtcat gagtaaatga gaaagagtac gttctccagc
     4321 cccttttcaa accgcagtga gcgaactcca tcgctgctcg acagcagcga attcagaatg
     4381 atgatgtccg gacgcgaagt aacggcgcga gtcaccagat ccttgccatc ggaatccacc
     4441 acctggtagc cgcgcgcttc cagcacatcg gtcagggtgc gaatcgtcga cgcgtcctcg
     4501 tccaccacca tcactttctt cttcgatttg ccctgcccca gcagcgaatc cacttcgtgg
     4561 aacagcgact tggtgtcgat cggcttggtc agatagcgat ccactccgag ccggaagcca
     4621 cgctccttgt cctccacgat ggaaagaatg atgatcggaa tatccatcgt cgcgggatcg
     4681 ttcttcagca ccgccgccac atcgaagccg ttcatctccg gcatcatcac gtcaagaatc
     4741 accaggcccg gcatctcttc gcgaatcagc gccagcgcct tgcgcccgtc ctcggcgagg
     4801 cgcacggcgt agccagcctc agtgagttcc tgctgcagca gcgaccgaat attcggatcg
     4861 tcatcgacca ccagcaccga tttcgaccgc ccgttctgcg ccaccacggt atcccgcaac
     4921 tggcgtacca gcgattcgaa gtccagagat cttctctccg gcagcaacgg cagttgtgcc
     4981 gacttcgcgg taatcggcag cgtgaacagg aaggtgctgc cccgtcccgg ctcgctctct
     5041 acccagatcc gcccgccgtg atactccacg atctccttgc agatcggcag cccaagcccg
     5101 gttcccttcg gtttgtcggt cagcgtgtcg ccgacctgtt tgaatttctc gaagaccttg
     5161 ctctgatcgc cggcggcgat cccaatgccg gaatccttca cgctgaccac cagttcgttg
     5221 tcgcgctggt gagccgcgca ggcaatcgaa cccgagtcgg tgaatttcac tgcattagaa
     5281 atcagattga tcacaacctg aatcaggcgg tcctgatcgc ccgttattgg tggcagatcc
     5341 aaatcgacat ctcgaatcag gtgcagcctc ttcgcttcaa agagcgatgc ggtggctgcg
     5401 gttgcgcgct cgatcacatc ggcaatcgag accgtgctca ggttccacgt gaacttgccg
     5461 gcttcaatct tggccagatc cagcacgtcg tcgatcagct tcgtcaggcg ctcgccctcg
     5521 ctcaccacca catcgaggtt ctcgatcacc tgctgcttgg cctgctgcac cttgcgatcg
     5581 tctccagtga tgagcgggaa aagcctttcc tgcaggcgtc gccgaataat cttcgcgaat
     5641 cccaaaaccg acgtcagcgg cgtccgcaat tcatgactca cggtcgacag gaatgaactc
     5701 ttcgccgcat ccgcttcttc ggccgccgcg cgcgcctggc gcgtttcgtc gaagagcctt
     5761 gcgttataga aagccacacc tacggctgaa gcaatcgtcg agagcagccg ttgatcggcc
     5821 tccgtgaacc gtccttcctg atcggtcgac tgcacgctga tgacgccaat cgcctggccg
     5881 ccggagtgaa tcggcactcc aagataggat gccgtcgacc gccccacttt ctcgattccc
     5941 agcttcgtcc gattggcgtc cagatcctcg ttgatgagca acggctgccc ggtgcggatg
     6001 atctgcgagg tcagtcccgt gccgaaaggg cgcgacggcg cctcttcgcc ccacgtgtaa
     6061 gggaagtgca gcatcatggt cgctcgatcg agcagcgcca gatacacaac cggcgcgtgg
     6121 aaaacatcgc gcacctgatc gcccacaaac tggatcagcc gatcccggtc cagctggctg
     6181 gccagcgcct gcgtgatgcg gttgatcgtc accagctccg cggcccgctc gctcacctcg
     6241 cgctctcgct ggttaatcgt ctgataggcg ttcgcgttgt cgagcgcgat cgtcgtatac
     6301 gccgccagat tctccagcag gctcagatgc tgctccgtgt aggcgttctt tttgaagctc
     6361 tgcacgctca gcacgcccag cacacgatcc tgtgcgatca gtggcagata gatcatcgac
     6421 gccggtggtt gcgcggcgct tccatcttcc agcgtccgag caccgtgatc gtacgaggcg
     6481 atgtatttcg aagactcggt cgcaacatcg ttcagcagaa tcggcttgcg atgatcgata
     6541 caccacaccg ccagctgatt cttgtcgtca gtgctgcgcg tgtagggtgc ataccgtatc
     6601 ccgttctcga tggcgaggct gtattcgatg agccgttttt ccggacgata aagcccgacc
     6661 ccgaagatgc tcgcatcgac aatctggttc acgcgctcat aaagccggaa caggatcgta
     6721 ctcagatcga gcgaggcggt gatctccttg ccgatgtcgc tcagcagctc gatgctttcc
     6781 ttctgcgccg aaatctctgt tgcctgtgcg cgaatctcct gcgtgcgcgc gctgaccgtt
     6841 gcctccagct cccttgcctg tgcctccagt tccaccgtgc ggcgacgcgc cttcgcgcgc
     6901 tcgtaacgga taatcgccgt ccagatcgcg aaggtgctca gcaaaaagag cagcgcatac
     6961 agaacgtagg ccagagtcga gcggtaccac ggaggcagaa tcgtgaaact gaaatcgcct
     7021 tcctcgccca cccgcccatc gtcggcccgc gagcgcacgc ggaaacgata cgttcccggc
     7081 cccagcccgc tgtagtttgc ctccttctgt ttgccccagt ccgaccagtc gtggtccgcc
     7141 ccttccagga ggtactgata actcgtgccg ctcgggttgc caaacgtgat ggcggcaaat
     7201 tcaaagcgca tcgcgctggt tccggccggg agccgcagcg tcggccgatc cgcaacctgc
     7261 acaccaccga acacggtctg cttgcccgca ctgacctggc ggatcagtgt ctggaagggc
     7321 tgagtggaac tggcggtggc gccaggattg aaccgcagca ggcgctctcc ggtgacccag
     7381 atgccgtccg cctcgttgtg catcaggaaa gtgcggaagc gcagcagaat ccggtaccga
     7441 tcttcgtcga cattccattt tccatcggcg ccgggacgga acagtcccag ccgtatgttg
     7501 tccgacgaag tcgtctcact ccagacgctg ccgtcgggat tgggcgacag aaccgccgaa
     7561 gcatccggac catcgacggg cagcagaaac gtgtcatcga caacgaagcg atgcgccgcg
     7621 tagtcccatc gatagatgtg cttcgcgcgg tccatcgtgg tatagagatc tttgcccacc
     7681 agctccgcat cggacgaccc ctccggcagg ccctccgctt tgccgaccgc ctcgaaatgc
     7741 gaatctcgca tgcccgtggg cgcgacatgt ccgcgcagca tcttgccgtc cgctccactg
     7801 acccacagat agccttccga atcctcagcg aagttgcggg cttcatagat caggccgggc
     7861 gcgcggcctt catcgaccca ggcatgaccg tcccagcgca tcgagcccac cccatccgag
     7921 tggccgatga acacgcgcga tggcgtaagc cgtgattgca ccagggcgta ggtctgttcg
     7981 gccagatcct gcacgcccgc catcgccgga accatctggt cgcccttcag cttcatcacg
     8041 ccttcgctgg tcgcgatcag cagctggtcc ggcgtcttgc ccgccggatc tttgaatacc
     8101 agcagggtcc agccctggtt cggatggccc tgcagcggta tcagcgtcgg tacgccggtc
     8161 tttgcatcga cgcccagcct gcccgccgcc gccgtggtgc cggttgtcgt cgtgtagacg
     8221 ttgccgttaa actgcgccac gtcgtaaatg ggaatggtgg agaagatcga gatgggcgag
     8281 ttgatctcca cgcgcgccac tccgccctcc gtgcccaccc acagcgcttt gtcgcgatcg
     8341 acaaaggccg agagtgcgtc cgggtccgga agtccggagt gctggtcgag aatctcccgc
     8401 agcttcccat cgtgatcgag gacggccatg ccgcccccgt tggtggtgac gccgatgccc
     8461 ccatccggca gaatcacgga cgtatacggc gggaacttgt ggaagaagtc atcggccccg
     8521 gttggaaacg gtttgacctt ctggccgtcg tacagcgtca gcggcttgcc gcgtgccgag
     8581 acgaggatct gaccgttgtc ccagggatag aggaagagct tgatggagtc cttgtacgca
     8641 tcgccgcccg ggacgggccg caattcatca ccgacaattt cctccaggcc aattccctgc
     8701 tgcgccgtat aaatatgtcc gcgcacttct gagagcgcct ggtaccgctg agccggctgt
     8761 tgcggcttcc acacctgcat gcgcgtgccg tcccaccgca gcagttcctc atacgagcga
     8821 aagaagactc catgctccgt tgccagagcc tgccagacat cggtgaaacc tcgatcctgg
     8881 gtggctatct tgtcgcgcag cgaaacaaac tgcagctgcc ccgttgcgtc cggagccaga
     8941 tagccaacgt tcccgttggc gcccagccag atcgtgccat cgttgtccag agccagcgat
     9001 cgaatcacgc tggttgccag aaagatcttg cgccaggtaa cgccgtcgta ttgcatcagc
     9061 gcgccgccgg aggcgccaat gtacatcagg ccgcggcgat cctgagcaat cgtccaaacc
     9121 tgaggccccc cgttgtagtc cttgaccgag tagaactgca ccaccggcat gccggcttcc
     9181 ggctttccgg gagccgcttc agcggcgctg cttgttcctg cgacagcgca gggcagcagt
     9241 gaagacaggg cgaggagcgc caccagccag agacgcggac ggatgcggag ggccaatcgg
     9301 ataccagggt cgatcagttg ctggggctaa gaatgaacag ggggaacttg aggcccgagc
     9361 ttaacatgca gaatcctcag gtgcaagaga tcgagacggc tccagaaagt ggccccggaa
     9421 cctgaaacga ctggaagcga agcctgtata ttgctccgtg acaggaccac tgagtcagag
     9481 gcccggcgaa ctctccggtt ggttacaagc gcctctggtg aacccatgtc ttcacccagg
     9541 gatccaaccc gcagagagtt cctgaccgtc ggttcagctg cggcgttggc cgctgctgcc
     9601 accgcggtgg gcgcccagac gaatgcactt ccgctggata cggcgaatct ctcttccgca
     9661 actgcggggt ccgacgatcc gttccatgtt cagtcccaac gaacattcag cggggagaca
     9721 gccacgcagg tggcgatgcc cctgggcggc atcggctctg ggtgcatctg cctgaacgga
     9781 tacggcggcc tgcaggattt ttcgatccgc acgcggcccg agacttcttc cctgccggaa
     9841 ggcttcacgt cggactcgcc cgaggcgggg ttcgcgttgc tccacatcaa aggcgccaac
     9901 agcgtcacca aactggtcga ggggccattt cctgtattca aaatcttcga tcagggactg
     9961 cagggccagg gattgcgccg cggaggtttc gaagggtttc cgcggttcgc caaagccacc
    10021 tttcgcggac agtatccctt cggcaaggta gatttcaacg atcccctcct tccgctgcat
    10081 gtgactctgg tcggctggaa tcccttcatc cccctggacg ataaaaactc cgggattccc
    10141 tgcgtgattc tggaatacag cttgtccaac aattccgcag cgccggtcga gtacgagttc
    10201 tcttatcacc tgtcgcattt agctcccgga tgccagccgg atcaggcggc cagcctgaac
    10261 acagtcattc ccggcaaggg cgtcttcctc cacaatcgcg aagccgccca tgctgaatcg
    10321 tatggcagcg caacgctcac agtcatcggc gccaccccgc gcatcaaggg gatgtggctg
    10381 cgcagcccag ggtgggagtt cgattcgctc tccgcgctct ggcgagaagt atcttccggg
    10441 aaattcacgg cgaacgacgg atcgaacgat gtggatacgg ccggcaggaa cggagcctcc
    10501 ctcctgctgg aaggcacgct ggcgcccggc gaatcgcgaa cttatccaat tgtcattgcc
    10561 tggcattttc ccaactgcta tctgcaggac ggtggcgtgt cagtagctgc caacgtgaag
    10621 ggcagcccag gatgccgcga tgtcaccagt gcgccgccct ggagaccatg gtatgtaacc
    10681 gcctggagcg acgcgcgcga cgtcgcggtt tacgtagaga aggagtacgc gtccctgcgc
    10741 cagcgcacca tgcagtttca cgacgcgctt ttctcttcca cgctgcctcg cagtgtcctc
    10801 gatgcggtct cggccaatct cggcattctc aagtcgccca ccgttctccg cgaagagaac
    10861 ggaaacctgt ggggctggga aggctgcttt cccgacagcg gctgttgcca cggatcctgc
    10921 acgcacgtgt ggaactacgc ccaggcgttt ccaaatcttt atccgcaact ggaacgcact
    10981 ctgcgcgaat tggaactcgt tcgttcgatg gacgaacacg gccatgtgaa tttccgcgga
    11041 gccattcccg atgggccggt accccacgat gggccagccg ccgctgacgg gcaactcggc
    11101 ggcatcatga agctttaccg cgactggcag atctccgggg acacggcatg gctcaataag
    11161 ctgtacccgc tggcgaagcg cagcctggat tactgcattc atcagtggga tccggatcat
    11221 cgcgga
//
LOCUS       DASZYN010000558         1820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_346997, whole genome shotgun sequence.
ACCESSION   DASZYN010000558 DASZYN010000000
VERSION     DASZYN010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346997"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1109)
                     /locus_tag="VHE33_14415"
     CDS             complement(213..1109)
                     /locus_tag="VHE33_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98693.1"
                     /translation="MTTEQNQPQPADNAAPAQAANQGQFNDEGGGGGRRRRRRRKRNR
                     NKGGAQANGQGQQQAQPQNQQPPPTFRPKPPKPQPSSQQRFFNNGNGNGQGGGRRRGK
                     QRRERTFVGPMDHSYRTVNGNVADSPPSTIEFRNGNVNGNGHYAEHQATWEPPPPPIR
                     EDAPTRILLFIEDLFFFAKINETARKLGIKVAFVKNEPETIARIFEAPEEERPSLIVF
                     DLNNAAAKPMTLIPKIKSKIKKGTSVIGFLSHLQGDLKAKALEAGCDMVMPRSAFSQN
                     LPNLLRRHGSDEEEHEMEHAQM"
BASE COUNT          337 a          521 c          577 g          385 t
ORIGIN      
        1 gcgctcgatc atgcgctcgc gatcgacaag cacacctatt acgagaagct gacgacggac
       61 gacgatttta aggacctggt ggcgagtccg gaggggcaaa agctgttgag tgcctataaa
      121 ccataacgcc ctcgaccgcc gagatccatg aggatcaaaa acaacaaagc cccggcgcag
      181 gccggggctt ttcctgttgc tgtggcgctt gcttacatct gcgcgtgttc catctcgtgc
      241 tcttcctcgt ccgacccgtg ccggcgcagc aggttgggca ggttttgcga gaaggccgaa
      301 cgcggcatga ccatatcgca gccggcttcc agtgccttgg ccttgagatc gccctgcagg
      361 tgggagagga atccgatgac cgaggttccc ttcttgatct tcgatttgat cttggggatc
      421 agcgtcattg gcttggccgc ggcgttgttc agatcgaaga cgatcagcga cggccgctct
      481 tcttccggag cctcaaaaat tctcgcgatg gtctcgggct cattcttcac aaaggcgacc
      541 ttgatgccga gcttgcgcgc ggtctcattg atcttggcga agaagaagag gtcctcgatg
      601 aagaggagga tacgggtcgg agcgtcctcg cggatgggag gcggtggcgg ctcccaggtg
      661 gcctggtgct cggcgtagtg gccgttgccg ttcacgttgc cgttgcggaa ctcgatggtc
      721 gatggaggcg agtcggcgac gttgccattc accgtacgat agctgtggtc catggggccg
      781 acaaaggtac gctcgcggcg ctgcttgccg cggcggcgtc ctccgccctg accgttgccg
      841 ttgccattat tgaagaagcg ctgctggctg gagggctgcg gcttgggcgg cttcggcctg
      901 aaggtggggg gcggttgctg gttctgaggc tgcgcctgct gctggccctg gccgttggcc
      961 tgggcgccgc ccttgttccg gttccgcttg cggcgacgac ggcggcgacg accgccaccg
     1021 cctccttcat cgttgaattg accctgattc gctgcctgcg ccggagcggc attatccgcg
     1081 ggctgaggct ggttctgctc tgttgtcatg cctgcacttc ctgtgcgact gtctgttgct
     1141 ggcgccggct tggagtactc gcctgggttg cgctgcgatt cgtgggtgac gcgcagcaac
     1201 cctgactcgc cgggagtcag cagcagtgaa aaattgttga gttccggcct acacctgctt
     1261 gaacgcaacg cacatggccg gaaggtttat acagcgacgc aacacgatgg actgggttcc
     1321 agggccccaa tggctcctgc tctacagctg gaggagtgag acgggggaac gcaaccggct
     1381 gccacgccga tcaacggaac gtactgaagt tgccgtccga tgccgattca accctcgcac
     1441 ccggtagctt ctctgcaaaa gcacaaacga cgacggagaa ggatgaaccg gggtgattcc
     1501 tgactctcga gaattgcagc gagcctcgcg gttttaccca gcgtggcatc agccgctcga
     1561 taggtaaaaa taatagtact ccctcacgag agaacctgac aagtcctttg atgcccccgt
     1621 ttggcgcagg gatccccaat tgggactatg gttatgcaca ggccgggtca gagcccgaaa
     1681 cagaaaagcc atccgatgga tggcttttcc gcgtgacagc cagtcactgg cctccctgaa
     1741 gttgcccttg acctggaact aagagaaccg atggatcagg acatttgttc attccggccg
     1801 gggcagaatc tcgaaggacg
//
LOCUS       DASZYN010000559         3337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_281733, whole genome shotgun sequence.
ACCESSION   DASZYN010000559 DASZYN010000000
VERSION     DASZYN010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281733"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VHE33_14420"
     CDS             complement(<1..318)
                     /locus_tag="VHE33_14420"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVT98694.1"
                     /translation="MRSSLITFLDDFRRLGDAPAVVVHRGNRRLVASWNQIADLADRF
                     AAELLRREIPIGERVVLWGANGLEWIGAFFGCIQRGVIVVPLDPAGALDFAGRVIDNT
                     RPRL"
     gene            complement(388..1845)
                     /locus_tag="VHE33_14425"
     CDS             complement(388..1845)
                     /locus_tag="VHE33_14425"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HVT98695.1"
                     /translation="MNHQLRFNLSAAARRSMIEHGFEPDYPPAIAAQLEQLRAHPPQP
                     DAGARDLRTLLWSSIDNDTSKDLDQIEFVETLADGNSRVLIGIADVDAFVPKGSPIDQ
                     HALRETTTVYTGVDIFPMLPEELSTGSTSLLQDQERLAVVGDFTIDAQAHVVAADIYL
                     ARVLNKAQLAYPSIGAWLSGQPAPPKVAASPDLQQQLHLQDQIAQRLRLERDTHGALN
                     LETIETHPIHLTDGQIDIEADTKNRATQIIEDFMIAANGAVARMLDTKGYSSIRRVVR
                     TPKRWERIVELAARRGTELPAEPDPKPLHNFLCEQQRKDPDHFADLSLAVIKLLGPGE
                     YILETPGQQPIGHFGLAVHDYTHSTAPNRRYADLVIQRIVKAMIAGKPNPYSDDELSS
                     IATQCTRMEDAERKVSREMQKRIAAVAMSSRVGQTFDAIVTGVNQHGSFVRTIKPPVD
                     GMLVQGAQGVDVGDRIHVSLVRTDPAQGYIDFARV"
     gene            1981..2805
                     /locus_tag="VHE33_14430"
     CDS             1981..2805
                     /locus_tag="VHE33_14430"
                     /inference="COORDINATES: protein motif:HMM:NF013397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HVT98696.1"
                     /translation="MPLRNIKVDRDAEKEAPQSQQRNLDRPTAVDIYEQVSRNARREL
                     QRPSIALAISGIVGGLTMGLTGLSVSVVSSILGNSPVAQFVALAFYPMGFMAVILGRG
                     QLFTENTLYPVALILAERRHFLATARLWGIVFPSNVAGAFLFAMLAARTKALQPDTLH
                     ALAQLGAQAASLPMRHIFWSGVVGGWMIAMVAWLVSGSHSITGSVAVIWLLAFIVGAG
                     HFAHCIATSGEILSAVLVHGVSPGGYGRWLLAATSGNILGGVVLVTLLEYGQVKLD"
     gene            2924..>3337
                     /locus_tag="VHE33_14435"
     CDS             2924..>3337
                     /locus_tag="VHE33_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98697.1"
                     /translation="MIGYIENNWHTLLWSAGILAGVFVIAHIVRSIVFWILIRLTQRH
                     GKLVGQSLVRHGKRPSRWIFPLLAMLCVLPGLELPQVLKSALEHITGLGLIAAIAWLV
                     ILFVDIARDVMASKYRVDVSDNLTARRIQTQFSMLH"
BASE COUNT          600 a          904 c         1176 g          657 t
ORIGIN      
        1 gagacgaggc ctggtgttgt cgatgactcg gccggcgaag tcgagggcac ccgcgggatc
       61 gagcggcaca acaatgacgc cgcgctgaat gcagccgaag aaagcgccga tccattcgag
      121 gccgttcgcg ccccagagaa cgacgcgttc gccgatggga atttcgcggc gcaggagttc
      181 ggcagcgaaa cggtcggcga gatcggcgat ctggttccag gacgcaacga ggcggcggtt
      241 gccgcgatgg acgacgacag ccggtgcgtc gccgaggcgg cggaagtcgt cgaggaacgt
      301 gatgaggctg gaacgcatga gatcgcaaac tgcaacaggc cgaccgcgaa cacgcggcca
      361 tttaggccac ccgcgcctgg attcagctta aacgcgggcg aagtcgatgt agccctgggc
      421 tgggtcggtt ctcaccagcg aaacatggat gcggtcgccg acgtcgacac cctgcgcacc
      481 ctggacgagc atgccgtcga ccggaggctt gatcgtgcgg acaaaggaac catgttgatt
      541 gacgccggtc acaatggcgt cgaaggtctg accgacgcgg ctgctcatcg cgactgcggc
      601 aatgcgcttc tgcatctcgc gtgagacttt gcgctcggca tcttccatgc gcgtgcactg
      661 cgtggcaatg gaggagagct cgtcgtcgga gtagggattc ggtttgcccg cgatcatcgc
      721 cttcacgatg cgctggatga cgagatcggc atagcggcgg ttgggcgcgg tggaatgcgt
      781 gtagtcgtgc acggcgagtc cgaagtggcc gatgggctgc tggccaggcg tttcaaggat
      841 gtattccccg gggccaagca gcttgatcac agcaagggaa agatcggcaa agtgatcggg
      901 atctttgcgc tgctgctcgc agaggaagtt gtgcagcggt ttaggatcgg gctcggcagg
      961 cagctcggtt ccgcgtctcg cagcgagttc gacgatgcgt tcccagcgtt ttggcgtgcg
     1021 gacgacgcgg cgaatcgacg aatagccttt ggtgtcgagc atgcgggcaa ccgcgccatt
     1081 ggcggcgatc atgaagtcct cgatgatctg cgtggcgcgg tttttggtgt cggcttcgat
     1141 gtcgatctgg ccgtcggtga ggtgaatggg gtgcgtctcg atggtctcca gattgagcgc
     1201 gccatgagtg tcacgctcca ggcgaagacg ctgtgcgatc tggtcctgca gatggagttg
     1261 ttgctgcaga tcgggcgacg ctgcgacctt gggaggcgca ggctgccccg agagccatgc
     1321 gccgatggag ggataagcaa gctgggcttt gttcagaacg cgggcgaggt agatgtcagc
     1381 agcaacgacg tgcgcctgcg catcaatggt gaaatcgcca acgacggcga ggcgttcctg
     1441 atcctggagc agcgaagtgg atccggtaga gagctcctcg ggcagcatgg ggaagatgtc
     1501 gacgccggta tagaccgtag tggtttcgcg cagagcgtgc tggtcgatgg gggagccttt
     1561 gggaacgaac gcatcgacgt cggcgatgcc gatgaggacg cgggaattgc catctgcgag
     1621 agtttcgacg aattcaatct ggtcgagatc cttcgaggtg tcgttatcga tggacgacca
     1681 gaggagggtg cgcaggtcac gggcgccagc gtcgggctgg ggaggatgcg cgcgcaactg
     1741 ttccagctgg gcagcaatgg ccggcggata atccggttca aaaccgtgct cgatcatgga
     1801 gcggcgcgcg gctgcgctga gattgaatcg gagctggtgg ttcatggccc tccctctgcg
     1861 gggcgaaggg caaggctatc acgagcggtg aacggggccg gcagcagttc agagggcagc
     1921 gcggcaaata ttcggggttg ccatccaacc ggcgggctca tccggcactc caaagcacga
     1981 atgccgctgc gcaacatcaa agtcgaccgg gacgctgaga aagaagcgcc gcaatcgcag
     2041 cagcgcaatc tcgatcggcc aacggcggtc gacatttacg agcaggtctc gcgcaatgcg
     2101 cgtcgcgaac tacagcggcc ctcgatcgca ctggccatct cagggattgt aggcgggctg
     2161 acgatgggcc tcacggggtt gagcgtatcg gtggtgagct cgatcctggg aaattcaccg
     2221 gtggctcagt ttgtggcgct ggccttctat ccgatggggt tcatggcggt gattctgggc
     2281 cggggccagc tgttcacgga gaacacgctg tatccggtag cgctgattct ggccgaacgg
     2341 cggcattttc tggcgaccgc gcgactgtgg gggattgtgt tcccgtcgaa cgtggcagga
     2401 gcctttctgt tcgcgatgct ggcggcgcgg accaaagctc tgcaaccgga cacgctgcac
     2461 gcgctggcgc agctgggcgc gcaggcggcg agccttccca tgcggcacat cttctggagc
     2521 ggcgtggtgg gcggctggat gatcgccatg gtggcgtggc tggtgagcgg cagccattcc
     2581 atcaccggct cggtggcggt gatctggctg ctggccttca ttgtgggcgc aggacatttc
     2641 gcgcactgca ttgcaaccag cggagagatt ctttccgccg tcctggttca cggtgtttct
     2701 cctggcgggt atggccgatg gctgctggct gcgaccagcg gaaatatttt gggcggggtc
     2761 gtgctggtga cgctgctcga gtatggccag gtgaaactgg attaatcggg cggggacgcc
     2821 ggtcccggga ttttccgccg agaatgcttt cgcgccctgg ctctgagcgt ttacccgaca
     2881 caaccgtcga cagcctgcat cccacacaga gaagggggtt gcattgatcg gttacatcga
     2941 gaacaactgg catacattgc tttggtcggc cggcattctt gccggcgtgt ttgtcatcgc
     3001 tcatattgtt cgcagcattg tcttctggat cctgatccgg ctgacgcagc gccacggaaa
     3061 gctggtcgga cagtctttgg tcaggcatgg gaaacgtcct tcgcggtgga tcttcccgct
     3121 gctcgccatg ctttgcgtgt tgccggggct ggaactgccg caggtgctca aatcggcgct
     3181 ggagcacatc acggggctcg gtctgattgc ggcgatcgcg tggctggtga tcctgtttgt
     3241 cgatatcgcg cgcgacgtga tggcgagcaa gtatcgcgtg gacgtttcgg acaacctgac
     3301 agctcggcgc atccagacgc agttctcgat gctgcac
//
LOCUS       DASZYN010000560         2366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_281815, whole genome shotgun sequence.
ACCESSION   DASZYN010000560 DASZYN010000000
VERSION     DASZYN010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281815"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1896
                     /locus_tag="VHE33_14440"
     CDS             73..1896
                     /locus_tag="VHE33_14440"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HVT98698.1"
                     /translation="MVLLAALWAGQQVHAASFYVDCTQSTPGDGSQEHPWNQIGPVSA
                     QMFHPGDLIALRRGTVCHGALTLHGSGRAEARIRLTAWGEGARPQIIADKNDTQALLL
                     KNAESWQIDSLDIAGGNTYGLLVTGDQDQVLSHITLRNLVVHDVFGGELHNKDNGLVV
                     FLRGSKRQRFDHVLIENVVAAHTNQWAGIMMGAGNFYSDEEGYNRDVTIRNSVAHDVY
                     GDGIILFRVRGGLIDASTAWLTGQQPTEKIGTPNAIWTWSCTDCIVRNSESFLTESPG
                     VDGGAYDIDWATTRNTVENNYAHDTQGYCVAVFGAGYVTHDAVVRHNVCVDNGLSPRM
                     ARLQGAIYIHTWNDGKIDGMIIDNNRIDWNPPAPAAPIVNDEGTELAGNPVTVRDNTI
                     RSTSPLLVRSVGDRLVFAHNIYMYAGLDKPSWFWNGKTWDSLAALQAAGGEKGSTLQA
                     DLEQSLSGTVARLANLPNLADLDGRAVPTQSASQYRLLLPIDLQLDADGLIPPEVMTR
                     LSVLRTLTREYNARQLQVLVFVSATGTNPSLRNALIDLDAPSIRFVRAESATVPVALV
                     DAKGRIVEQWSGSDRFNAATVGYAVRRRLGVPVYAQMDARP"
     gene            1899..>2366
                     /locus_tag="VHE33_14445"
     CDS             1899..>2366
                     /locus_tag="VHE33_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98699.1"
                     /translation="MSRPSRIALVLIACLGLASLAHAADDSSRISANPDFAEGLRGWQ
                     IQGDVHVGAAPGNAAKHTVTLGPGAASIVQHVAAGGANHMMIEATLHAVPAGLATVSV
                     RCLDKDGRELMTLRSPTDIRPGKEADTLADYFRPHPLTASVEISVAKDDTSATV"
BASE COUNT          453 a          785 c          686 g          442 t
ORIGIN      
        1 ccgctgccct gcacgacaat aatcaccatt agcatgcacc ctttccccgt ttcccgatct
       61 gtacgtgttg cgatggttct gctggccgcc ctctgggccg gtcagcaggt ccacgctgca
      121 tccttctatg tcgactgcac ccagtccacc cctggcgacg gcagccagga acatccgtgg
      181 aaccagatcg gtccggtctc agcgcaaatg ttccaccccg gcgacctcat cgctttgcgg
      241 cgcggcacag tttgccacgg agctttaaca ctgcacggtt cggggagagc ggaggcgcgc
      301 atccgcctca cagcatgggg cgaaggtgcg cggccacaga tcatcgccga taaaaacgat
      361 acccaggctc tgctcctgaa aaatgccgaa tcctggcaga tcgattcgct cgacatcgca
      421 ggcggcaata cctacggtct gctcgtcacc ggcgaccagg accaggtgct cagccatatc
      481 accctccgca accttgtcgt ccacgatgtg ttcggcggcg agctccacaa caaggacaac
      541 ggcctggtcg tcttcctgcg cggatcgaag cgccagcgtt tcgatcacgt gctcatcgaa
      601 aacgtggtgg ccgcgcacac caatcagtgg gccggcatca tgatgggcgc cggcaacttc
      661 tactccgacg aagagggcta caaccgcgat gtgacgatcc gcaacagcgt cgcccatgat
      721 gtttacggcg acggaattat cctgtttcgc gtgcgcggcg ggctgattga tgcgagcacc
      781 gcctggctga ccgggcagca gccgacagaa aaaatcggca cgccgaatgc catctggacc
      841 tggtcctgta ccgactgcat cgtgcgcaac agcgagagtt tcctcaccga aagtcccggc
      901 gtcgacggcg gcgcctacga cattgactgg gcaactacgc ggaacacggt cgaaaacaac
      961 tatgcgcacg acacgcaggg gtactgcgtt gccgttttcg gagcaggcta cgtcacgcac
     1021 gacgccgtgg tgcgtcacaa tgtttgcgtc gataacggtc tgagcccgag gatggcgcga
     1081 ctccagggag cgatttacat tcacacctgg aacgatggaa agatcgacgg catgatcatc
     1141 gacaacaatc ggatcgactg gaatccgccg gcgcctgccg ctccgattgt gaatgatgag
     1201 ggaaccgagc tggcggggaa cccggtgacg gtccgcgaca acaccatccg ctccacgagc
     1261 cctctgctgg tgcgcagtgt gggcgaccgg ctcgtgtttg cacacaacat atatatgtat
     1321 gccggactgg ataagccttc ctggttctgg aatggcaaga cctgggattc cctggcggcg
     1381 ctgcaggccg caggtgggga aaagggcagc acgctgcagg ccgatctgga gcagtccctg
     1441 tcgggcaccg tcgcacgcct cgccaacctc cccaacctcg ccgatctcga cggtcgtgcc
     1501 gtccccaccc aatccgcgtc acaataccgg ctcctcctcc cgatcgatct ccagctcgat
     1561 gccgatggcc tgatcccgcc cgaggtcatg acccggctca gcgtgctgcg tacgctcacg
     1621 cgcgaataca acgcccgaca actgcaggtt ctcgtatttg tatccgccac cggcacaaat
     1681 ccatcattgc gtaatgccct gatcgatctt gatgcgcctt cgatccgttt cgtccgggcc
     1741 gaatcggcta ccgttcccgt agctctcgtc gatgcaaagg gacgtattgt ggagcagtgg
     1801 tccggctcgg atcgtttcaa tgctgccacc gtgggctatg ccgttcgccg gcggctcggc
     1861 gtacccgtct atgcccagat ggatgcgcgt ccctgaccat gagcaggcct tcgcgcattg
     1921 ccctcgtcct gattgcgtgc ctcggcctcg cgtccctggc gcacgcagcc gacgactctt
     1981 cgcgtatctc cgccaatcct gatttcgccg aagggctgcg gggctggcag atccagggcg
     2041 acgttcatgt cggtgccgct ccggggaatg cggcaaagca caccgtcact ctcggtccgg
     2101 gcgcagcctc gatcgtccag catgtcgcgg ccggcggcgc gaatcacatg atgatcgaag
     2161 caacgctgca cgcggtgcca gctgggttgg cgaccgtgtc cgtccgctgt ctcgacaaag
     2221 atggccgcga gctgatgacc ctgcgctcgc cgaccgacat caggcctgga aaagaggccg
     2281 atacgctcgc ggattatttt cgcccgcatc ctctcaccgc cagcgtcgag atcagcgtcg
     2341 ccaaagacga cacctcggcc accgtc
//
LOCUS       DASZYN010000561         4086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_32244, whole genome shotgun sequence.
ACCESSION   DASZYN010000561 DASZYN010000000
VERSION     DASZYN010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32244"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VHE33_14450"
     CDS             <1..403
                     /locus_tag="VHE33_14450"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HVT98700.1"
                     /translation="FPFLTKKRTQCGFFCPFGAFQSLFNKINLFEIRFDRTKCVDCTL
                     CQTACPTMALSAESIREGKTLLNCMKCGACVDSCKKDAAVWHLKGTPVAVKPERARLL
                     FLYGTWALATMLGGSIMANSLATILGFLPIR"
     gene            418..942
                     /locus_tag="VHE33_14455"
     CDS             418..942
                     /locus_tag="VHE33_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98701.1"
                     /translation="MRTRILAFLSYAGAAVSLIVAACTPFVLLGAFSQAVARTGIHVD
                     AVYSGGTIARTVLRGAWQIDIYRPVEPRALQDADPFVQIAFRPATALPRRFTDEVDLN
                     GDGRPDIRVQLLIPKDPSARPSGEVIALNPIYRSFRMPGRMPGTEIGFSELIAQSNGA
                     VLVRVPIQKRSVPR"
     gene            1066..1557
                     /locus_tag="VHE33_14460"
     CDS             1066..1557
                     /locus_tag="VHE33_14460"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HVT98702.1"
                     /translation="MKNENLRELLVEELKDLYSAENQLTKALPKVAKNASDPELKKAI
                     ESHLKETEGHVARLEQIFEKLEGSPRGKTCEGMKGLITEADERIKEGGEPAVLDAGLI
                     ADAQRVEHYEISAYGSARTFANLLGEKEIARLLEQTLKEEKAADEKLTSIAESINVEA
                     KAA"
     gene            complement(1691..1891)
                     /locus_tag="VHE33_14465"
     CDS             complement(1691..1891)
                     /locus_tag="VHE33_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98703.1"
                     /translation="MVYTAGQQVRILHSGAIGRVFLKLPLRDVYLVQFPGFPVVEKIY
                     YGCDLEPVSTAAAVHEEELHHV"
     gene            complement(2054..2878)
                     /locus_tag="VHE33_14470"
     CDS             complement(2054..2878)
                     /locus_tag="VHE33_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98704.1"
                     /translation="MKTRTLISVALSSLFASSAMVAAAQDKTPPPPPKVLVIQREYLK
                     PGKAGNVHVQSEAAFIKAENDAKWPTHYIAMDSMSGPTRALFMFAYDSFDAYGKDQEA
                     QAKNTDFAAAIDAAQLADGELLARYDASAFVYHPEMSLHASVEVPHQRYWEFMTFRIK
                     AGHDKDWADLTKLYVDGFAKTDSHWATYESEYGENNGGVWIVINPMRSLAEVDKGMTN
                     GAAFMAALGPANMKRLADLTAACVDSVQVNLFAVNAKESYFDPSWSTTAPEVYGQQ"
     gene            complement(3175..3528)
                     /locus_tag="VHE33_14475"
     CDS             complement(3175..3528)
                     /locus_tag="VHE33_14475"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVT98705.1"
                     /translation="MSHIAAPPVFTFEVEECPDGVAIVHCHGKLVAGVTDVVITGVRP
                     LFANHKRVVLDLSDLKHTDSMGMGALVRLYVGAKSAGCSLELAHLSKQIRHLLGITHL
                     LDVFVIVGESGIKWM"
     gene            complement(3616..>4086)
                     /locus_tag="VHE33_14480"
     CDS             complement(3616..>4086)
                     /locus_tag="VHE33_14480"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HVT98706.1"
                     /translation="PSRAIVLIAAPGREHEAALRLGRIGFDRVEGYLRDGMAALTAQP
                     ELLAYTRRSSAPELAALMAAAHPPLLIDVRTPREWSTRHVDGSLNMPLSRLRDHLAEI
                     PRDRAIAVQCAGGYRSSIAASMMQQQGITDLMEVAGGLAAWEAAALPVASSPVA"
BASE COUNT          834 a         1289 c         1214 g          749 t
ORIGIN      
        1 cttccctttt ctgacaaaga aacgaacaca gtgcggcttc ttctgcccct tcggcgcgtt
       61 tcaatccctg tttaacaaga tcaacctctt cgagattcgt ttcgaccgca cgaaatgcgt
      121 cgactgcacg ctctgccaga ccgcttgccc caccatggcc ctctctgccg agtcgatccg
      181 cgaaggcaaa acgttgctga actgcatgaa gtgcggcgcc tgcgtcgaca gctgcaaaaa
      241 ggatgcggcc gtatggcatc tcaaaggcac tccggtcgcc gtcaagcccg agcgggcgcg
      301 tctgctgttt ctgtacggca cgtgggcact ggcaaccatg ctgggcggca gcattatggc
      361 caactcactg gcaaccatcc tcggctttct gccgatccgc taacaaggag acactgcatg
      421 cgtacacgga ttctggcgtt cctttcctat gcaggtgcag ctgtatccct gatcgtcgct
      481 gcctgtactc ccttcgtgct gctcggggcc ttctcccagg ccgtggcgcg gaccggcatc
      541 catgtcgacg cggtctactc cggaggcacc atcgcgcgca ccgtcctacg gggcgcctgg
      601 cagatcgaca tctatcgccc cgtcgagccc cgcgctctgc aagacgcaga ccccttcgtg
      661 cagattgcct tccgccccgc taccgctctg ccccggcggt tcactgacga ggtcgacctc
      721 aacggcgacg gccgtccgga catccgcgtg cagcttctca tcccaaaaga tccgagcgca
      781 cggccgtccg gcgaagtcat cgcgctcaac ccgatctacc ggtccttccg gatgccgggc
      841 cggatgccgg gaaccgagat cgggttttcc gaactgatcg cgcagagcaa cggcgccgta
      901 ctggtccgcg tgccgattca gaagcgcagc gtaccgcggt aactccgcga tcgaagccgg
      961 catcccattc agcaagccgg ccgcagaggc ggcctgccgg gcccgccctc ctgttgcgcg
     1021 aatccggcgc gatctgcatt tcatccccaa ggagaaccac ttatcatgaa gaacgaaaat
     1081 ctgcgcgaac tcctcgtcga ggagctgaag gacctgtatt cggccgagaa ccagctcacc
     1141 aaagcgctgc ccaaggtcgc gaaaaacgct tccgatcctg aactcaaaaa ggcaatcgag
     1201 agccatctga aagaaaccga agggcacgtg gcacggctgg agcagatctt tgagaaactc
     1261 gaaggcagcc caaggggcaa gacctgtgaa ggcatgaagg gcctgatcac cgaagccgac
     1321 gagcgtatca aggaaggtgg agagccggcg gtgctggacg cgggtctgat cgctgacgcc
     1381 cagcgcgttg agcactacga gatctccgcc tacggctccg cgcgcacctt cgcgaatctg
     1441 ctgggcgaga aagaaattgc ccgcctgctg gagcaaacac tgaaagaaga aaaggcggcc
     1501 gacgagaagc tgacgtcgat cgccgagagc atcaacgtcg aagcgaaagc cgcctgagta
     1561 cgttcagtcc ggacacgaaa aagaggggag gctccggcct cccctctttt ttcgcgattg
     1621 ctaaccgatt tttagccgac cggacctgag ctggccggac ttcatttaac cggactttgg
     1681 ctaacgtcag ctaaacgtga tgcagttcct cctcgtgcac cgcagccgcg gtgctgacag
     1741 gttccagatc gcaaccgtag taaatcttct cgacgaccgg aaaaccaggg aactggacga
     1801 ggtagacatc gcgcagcggg agtttcagga agacccggcc gatggctcca ctgtgcagaa
     1861 tgcgtacttg ctgccctgct gtgtagacca taccgttgga aaatctaggc ctgggcagag
     1921 acagttcgga atcccctgag agtgtcaaac cgcggacacc ttgggcatct tgctaaatgg
     1981 ttaccgtgcg agtatcgacg gagaagccca tgtcgcccgg cgacggagac atgggcttcc
     2041 ctgcggaagc ggcctattgc tgaccgtaga cttcgggggc cgtggtcgac cagctggggt
     2101 cgaagtaact ctccttagcg ttgacggcaa acaggttcac ctggacggag tcgacgcagg
     2161 ccgcggtcag gtcggcgagg cgtttcatgt tggccgggcc gagggctgcc atgaaagcgg
     2221 cgccgttggt catgcctttg tcgacttccg cgagcgagcg catggggttg atgacgatcc
     2281 agacgcctcc gttgttctcg ccatactcgc tctcataagt ggcccagtgc gagtccgtct
     2341 tagcgaagcc atccacatag agcttggtca ggtcggccca gtccttgtcg tggccggcct
     2401 tgatgcggaa cgtcatgaac tcccagtagc gctggtgcgg gacttccacc gacgcgtgca
     2461 ggctcatctc cgggtgataa acgaacgcgc tggcgtcgta ccgtgccagg agctcgccat
     2521 ccgccagctg cgccgcgtcg atcgcagcag cgaaatcggt gtttttcgcc tgcgcttcct
     2581 gatctttgcc ataagcgtcg aaggagtcgt aggcaaacat gaacagagcg cgcgtcggcc
     2641 ccgacatcga atccatcgca atgtagtgcg tcggccattt tgcatcgttc tcagccttaa
     2701 tgaacgcggc ttccgactga acatgcacgt tgcccgcttt gccgggcttc aggtattcgc
     2761 gctgaatgac gagaactttc ggtggaggtg gcggagtctt gtcctgagcc gccgcaacca
     2821 tcgcggacga cgcgaacaac gaggaaagcg caacggagat gagcgtgcga gttttcatgt
     2881 ggatggccct ccctggggcg aagtctctcc gccaatcgat cccggcgccg gcaggaatcg
     2941 gcccgacgct ggtgcagcgg agacgtgggt gagtcagagc gcctcaggaa aagctgggcc
     3001 gggcgctgcc taccttaact gcgggggaac gtgggttctg ctctcgcgga agtgggcaga
     3061 atgaagcgct ggagaggctt ccgggagcac cggtatactt ccgcgctctg caggccttgt
     3121 caagatgact gcgatgctat ttgtcgcctc gccatgggcg aagcaacacg ccgatcacat
     3181 ccacttgatg ccgctctcgc cgacgatgac gaagacatcg agcagatgcg taatcccaag
     3241 aagatgacgg atttgtttgc tcagatgcgc cagctccaaa ctgcaaccgg cggacttcgc
     3301 gccgacgtag agacgcacca gcgcccccat gcccatgctg tcggtgtgct tcagatcgga
     3361 aagatcgagc accacgcgct tgtggttcgc gaacagcggc cgaacgccag tgatcacaac
     3421 gtctgtgact ccggcgacca gcttcccatg gcaatgcaca atcgcaacac catcgggaca
     3481 ttcttccact tcaaaggtaa acaccggagg cgcagctatg tgagacatga cgtgtcctca
     3541 tccatggcgt tgcagcagga atcgagcgga gtccagcgat tatcgcacag caacacgccg
     3601 aattttgccg aaccgtcagg ccactggact cgacgccacc ggcagtgccg cggcctccca
     3661 ggccgccagc cctccggcca cttccatcag atcggtgatt ccctgctgct gcatcatgct
     3721 cgcggcaatt gacgaccgat acccgcccgc gcactgcacc gcgatcgcac gatcgcgggg
     3781 aatctccgcg agatgatcgc gcaggcggct gagcggcatg ttcaggcttc cgtccacgtg
     3841 gcgcgtcgac cattcgcgcg gcgtgcgaac gtcaatcagc aatggcggat gagcagcagc
     3901 catcaaagcg gccaattccg gagcactcga ccggcgcgtg taggccaaaa gctccggctg
     3961 agccgtcagc gctgccatgc cgtcgcgaag atatccctcc acccggtcga acccgatgcg
     4021 gcccagccgc agcgcggctt cgtgctcgcg ccccggcgct gcgatgagca cgatcgcgcg
     4081 gctcgg
//
LOCUS       DASZYN010000562         2693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_347505, whole genome shotgun sequence.
ACCESSION   DASZYN010000562 DASZYN010000000
VERSION     DASZYN010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347505"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VHE33_14485"
     CDS             <1..264
                     /locus_tag="VHE33_14485"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HVT98707.1"
                     /translation="AQQASLTVADAIDAAHNKQMIDPFQKLKGAAFDRKFAQEMVAGH
                     TKAIAVYKNEADNAQNPAIKSYAQNALPVLQKHLDSAKDLGKK"
     gene            complement(358..933)
                     /locus_tag="VHE33_14490"
     CDS             complement(358..933)
                     /locus_tag="VHE33_14490"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HVT98708.1"
                     /translation="MARTESAMLALGTQIPAFSLKDVRTNQLHTHSSLIGPKGLVVMF
                     ICCHCPFVKHVEKQLAQLAVDFRDSGIGFVAISSNDAENYPDDAPASLAEQALKLGFT
                     FPYLYDKTQEVARSFDATCTPDFFLFDGSGSLVYRGQLDSSRPGNDIPVTGRDLRAAI
                     KAVVAGEPISQDQLPSLGCNIKWKNEWLNRL"
     gene            1089..1553
                     /locus_tag="VHE33_14495"
     CDS             1089..1553
                     /locus_tag="VHE33_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98709.1"
                     /translation="MGPLCSSSAFDVRERFSKLAVCVASLLILLLAMPPQAFAKAKPL
                     DASAVHSRILKRGLGNPVGVEMSNGVALIGRIIAINTDSFTLQLFNDPDPVTINYADV
                     IDLRTGPSHGFWIVTAVGIGATAGLAIWGFHHVHSLEQQNQIPNMPPFPTVP"
     gene            1582..2250
                     /locus_tag="VHE33_14500"
     CDS             1582..2250
                     /locus_tag="VHE33_14500"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HVT98710.1"
                     /translation="MSAKQRFQGTLRPDGTGLNWNVITVPFDPAKVWPKRQRLRVRGT
                     INGFAFRTSLFHARDGSYILLVNRKMQKGGSVRRGHIAEVVLEPDLEERTQTTPPELE
                     KILKQDRSLKKFYEQLSDSMRKAIADQIEQPKNAEVRSGRAELWAERMMLAMEGEKET
                     PPILELAFQRNPRARAGWKAMTPIQRRGHLLGIFYYQSPESRQKRAQKAIEEAVRAAD
                     KHRT"
BASE COUNT          579 a          785 c          826 g          503 t
ORIGIN      
        1 gcgcagcagg ccagcctcac cgtggctgac gccatcgacg ccgcgcacaa caagcagatg
       61 atcgatccct tccagaagct caagggcgcc gccttcgacc gcaagtttgc gcaggagatg
      121 gtcgctggac acaccaaagc cattgcggtg tacaagaacg aagccgataa cgcccagaac
      181 ccggcgatta agagctatgc gcagaacgcg ctcccggttc tgcagaaaca tctcgacagc
      241 gcaaaggacc tcgggaagaa atagctggtc gcttccagca tcgtttggtt cttcacgcgg
      301 gcgttcatct gtgaaagggc ccggcttttg ccgggccgtc acctgaacac ggccgcctca
      361 gagccgattc agccattcgt tcttccactt gatgttgcag ccgaggctcg gcaattggtc
      421 ctggctgatg ggctcgcccg cgacaacggc cttgatcgcg gcgcgcagat cccggccggt
      481 cacgggaatg tcgttccccg gccggcttga gtcgagctga ccgcggtaga ccagcgagcc
      541 cgagccgtcg aacaggaaga agtccggcgt gcaggtcgcg tcgaacgacc gcgcgacctc
      601 ctgggtcttg tcgtagaggt aaggaaaggt gaagccgagt ttcagagcct gctcggccag
      661 cgacgccgga gcatcgtccg gatagttctc ggcgtcgttg gatgaaatgg ccacgaaccc
      721 aatgccggag tcgcggaagt caaccgccag ttgcgccagc tgcttttcga catgcttcac
      781 aaaagggcaa tggcagcaga tgaacatcac caccagtcct ttggggccga tcagagagga
      841 atgggtgtgg agttgattgg tgcgaacgtc cttgagagaa aaggccggaa tctgcgtgcc
      901 cagcgccagc atggcggatt cagtgcgagc catcgatgag gacctccaac agcctctagg
      961 gtaacgcgca cgcgtcgcac tgtgaccgct gcgaaacacg aaactatttc gacagtgttt
     1021 cgtcttcgtg gacagcagtg gagcgaatgg gaatcgatga acgagacgtc cactgctgag
     1081 gtgcgagcat gggacctctt tgcagttctt ctgcctttga tgttcgcgaa cggttttcga
     1141 agctggccgt ttgcgtcgcg tccctcctga ttcttctcct tgctatgcct ccccaggcat
     1201 tcgcgaaagc gaagccgctg gacgcctctg ccgtccactc ccgcatcctc aaacgaggac
     1261 tcggcaaccc ggtaggcgtg gagatgagca acggtgtggc tctgatcgga agaatcatcg
     1321 ccatcaacac cgactccttc acattgcagc ttttcaacga tccggatccg gtcaccatca
     1381 actacgccga cgtgatcgat ttgcgaaccg ggccatccca cgggttctgg attgtgacgg
     1441 cggtggggat cggtgctacc gcgggcttgg ccatctgggg cttccaccac gtgcacagtc
     1501 tcgagcagca gaatcagatt cccaacatgc cgcccttccc gaccgtcccg tagccggtgc
     1561 gctgcacgct atgctgggag cgtgagcgcg aagcagaggt ttcaaggcac tctgcggccc
     1621 gacggcacgg gcctgaactg gaacgtcatc accgttccgt tcgatccggc aaaggtgtgg
     1681 ccgaaacgcc agcgcctgcg cgtgcgcggc accatcaacg gtttcgcctt tcgcacctcg
     1741 ctgttccacg cgcgcgacgg cagctacatt ctgctggtca acagaaagat gcaaaagggt
     1801 ggcagcgtcc gccgcggaca catcgcggag gtcgtgctcg agcccgacct cgaagaacgg
     1861 acacaaacga ctccgcctga actggagaag atcctgaagc aggaccgatc gctgaaaaag
     1921 ttctacgagc agctcagcga ctcgatgaga aaggcaatcg ccgaccagat cgagcagccg
     1981 aagaatgcgg aggtgcggtc ggggcgagcg gaattgtggg ccgaacgcat gatgctggcc
     2041 atggaaggtg agaaggagac gccgcccatc ctggaattag cctttcagcg aaacccgcga
     2101 gcgcgcgctg gctggaaggc catgaccccg atccagcgtc gcggccattt gctggggatt
     2161 ttctactacc agagtccgga atcccgtcag aagcgagcgc agaaagcgat tgaggaagcg
     2221 gtgcgcgcag cggacaaaca tcgaacctga ggcagggcag cttccagcgc aaccgatcgt
     2281 ttccatcctg tttcctggca gcggctaacg cggattccgt cgttcatcag tgcgatacgt
     2341 gcccctcact ggcgctcaaa ggcaacaggt cctgccgggg ctccgagctg cgggtggacc
     2401 cgcgtcacgt cccggctgtc gataaggggg gcgatccggg cggttcagag gacttgattc
     2461 accgcaaatc aaaggattta aggcaaatgg gcttgttttg tcgcagggaa ggaggcggca
     2521 ggctgagagc ggggcatcgg tacgtgagtc atcggccaca tttaatactt agataatacc
     2581 gccggggaaa tattcagggg catgttaaca gctatagtcc tctttgtatg gcggagtctg
     2641 taccacagcg gtgggcagag tcatacaact tttgttgagg agtacttcag tca
//
LOCUS       DASZYN010000563        19444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_89046, whole genome shotgun sequence.
ACCESSION   DASZYN010000563 DASZYN010000000
VERSION     DASZYN010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 19444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19444
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89046"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VHE33_14505"
     CDS             complement(<1..725)
                     /locus_tag="VHE33_14505"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HVT98711.1"
                     /translation="MSEIQTVGVIGAGTMGNGIAHIFARSGFRVLLCEVERRFLDRGL
                     ETIRKNLGREAEKGKITVNDVDDALARIEGTLDRTDLAEANFIVEAATEKFEIKKALF
                     QELDTIAKPDVILASNTSSISITKLAAVTGRPERVIGMHFFNPVPVMKLVEVIRGLQT
                     SQETYETVATLAEKLGKTAVEVNDAPGFVSNRVLMPLLNEAMYAVMEGVATPDAIDEV
                     FRLGMAHPMGPLTLADFIGLDVCL"
     gene            complement(732..1127)
                     /locus_tag="VHE33_14510"
     CDS             complement(732..1127)
                     /locus_tag="VHE33_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98712.1"
                     /translation="MNTRVLMIASAAAMGFAGLALTFAPQEVLAWGGAEPRGFAVLMA
                     QAAGALYAGFAMLNWMAKDVAIGGIYSRPVAVGNFAHFCLMGGALVRLSVSAQRSGFI
                     LLVALLYLVFAIGFGLLLFISPRSVAKTE"
     gene            complement(1156..1983)
                     /locus_tag="VHE33_14515"
     CDS             complement(1156..1983)
                     /locus_tag="VHE33_14515"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HVT98713.1"
                     /translation="MNEVGREDDEKIERFVYRESGSATRVMMTAKAAARTSKGRVRTG
                     NEDAFGMRPEHGIYVVCDGMGGAAGGEVASHMAVDTVLDRMSANPAATPGRDGLHDAI
                     AEANRLVLERAEREPQLSGMGTTLVALLLENSHALVAHAGDSRCYLLRAGKLERVTQD
                     HSLVDEQMRLGAMTAEEAERSPLRSVITRAVGTQPSVTEDVSEIELQAGDVFLLCSDG
                     LTREVDEGGIARILGAGRDLDRVAQALVDAANDAGGRDNVTALVVQVQPVAGSEPAE"
     gene            2129..2881
                     /gene="tpiA"
                     /locus_tag="VHE33_14520"
     CDS             2129..2881
                     /gene="tpiA"
                     /locus_tag="VHE33_14520"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HVT98714.1"
                     /translation="MPRKPLIAANWKMYKTPAQAQEFFKNFLPLVANHERDEIVVCPS
                     YTSIPVVMAAATGSGVAIGAQNMHFADEGAYTGEISPVMLKAIGPTHVILGHSERRQY
                     FAETDELINKKLKAALQHQLIPIVCVGEVLAEREAGKTNEVLVRQTRGVLAGITAADA
                     APIVIAYEPVWAIGTGKTATPQIASEAHAVIRAEVAKLLGQEAADAIRILYGGSVKPD
                     NSPALMAEAEIDGALVGGASLDPQSFAKIVNY"
     gene            2903..3349
                     /locus_tag="VHE33_14525"
     CDS             2903..3349
                     /locus_tag="VHE33_14525"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HVT98715.1"
                     /translation="MNEPATIREILENTRTIAVVGLTNREGRASLGVSRFMQSQGYRI
                     IPVNPQVEEALGEKAYPTLDDAVAAHPEIDLVNVFRRPEHVPEIVKDAIRLKIPRLWL
                     QEGVCHDEAVGWAEAAGIKVVQDHCILKERMAAGWRGRSESTIGRS"
     gene            3509..4039
                     /locus_tag="VHE33_14530"
     CDS             3509..4039
                     /locus_tag="VHE33_14530"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HVT98716.1"
                     /translation="MPTQIEPLLGQNAEPDPTFFVESWTIGVTGASENYLRRHQHEKD
                     HPAPAPMSFPSYSFVTFLNVDPHTPIPTQSSPKTSMSDGHSFARRTRSEEDNEAFVPG
                     PLTFQSACRLLGVTATSTRDEIRAAYRKMASRYHPDRLAAAGVGDSQFASDRMASINE
                     AYRLLCDSAVAQSALR"
     gene            complement(4065..4589)
                     /locus_tag="VHE33_14535"
     CDS             complement(4065..4589)
                     /locus_tag="VHE33_14535"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HVT98717.1"
                     /translation="MRLVRFSAAVSLDGFIAGPKGESDWIVHDPDVDFSAMMAEFDTF
                     LIGRKTFELMVKAKRDQPPKGVEYVVFSRTLRAAEFPRVRIESDPGSVVGDLRSRRGK
                     DLWLFGGGELLGSFLAAGLVDRIEVSVIAALLGAGIPMVPAKAGPTKLKLLGHRVYPK
                     TGTLRMEYEVVRTE"
     gene            complement(4610..5029)
                     /locus_tag="VHE33_14540"
     CDS             complement(4610..5029)
                     /locus_tag="VHE33_14540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HVT98718.1"
                     /translation="MTERTHPIRVLVAKPGLDGHDRGAKVIARALRDAGMEVIYTGLR
                     QTPEMIVSAAIQEDVDCVGLSILSGAHNAIVPRVTAMLREQGAADILVVLGGTIPEQD
                     IAALKESGVSAVFGPGTSLESVVRFIRENVKAKGVGV"
     gene            complement(5065..6324)
                     /locus_tag="VHE33_14545"
     CDS             complement(5065..6324)
                     /locus_tag="VHE33_14545"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HVT98719.1"
                     /translation="MPVFALVSTLVCLAAAFSLVSRRVLHLPVTVGVMLQSLGMSLVF
                     VFVGRAFPGLHATVAAEVARIDFSAVVLHGMLAFLLFAGALKLDLKELAKQKASVTAL
                     SVFGTLLSTAIVAALLKLILTATGFHATWIACLLFGALISPTDPIAVLELLRRVDAPA
                     SLEAQLTGESLFNDGVGAVVFLTLIDTINGGGRPSVASFTWLLLIKAGGGVILGLALG
                     YMVYRLIRTVDSYRVEVMLTLALAMGGYALADALRLSAPLEAVAAGLVLGGRARHLGM
                     SEETRGHVDDFWDLIDAFMNVILFLLLGLELLAMPWNIEYVYAGLLAIPVVLGARWLS
                     VGASLGMVRAFHKPVRGAMSVLTWGGLRGGLAVALALSVPQGRPHDRLLAITYIVVIF
                     SIVVQGLTMSTLLHRLGLGRAHPVEVP"
     gene            complement(6334..6882)
                     /locus_tag="VHE33_14550"
     CDS             complement(6334..6882)
                     /locus_tag="VHE33_14550"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVT98720.1"
                     /translation="MKRYILTGAPGSGKTAVLRQLELDGFGVVEEAATDLIALRQAEG
                     MAEPWMQAGFVEDVAWLQRLRLERASKSGDAVQFHDRSIFCTLALAKYLAVPCPAELI
                     REAERVREECVYERSVFFLQNLGFIEPTAARRITFEETVRFERIHEEVYREWGFEPVT
                     VEAAPLEERVRTIGAFIELSAR"
     gene            complement(6907..7458)
                     /locus_tag="VHE33_14555"
     CDS             complement(6907..7458)
                     /locus_tag="VHE33_14555"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HVT98721.1"
                     /translation="MRARKPAAPLNENELFDYAVKLLGQQMRTVAEVKRLLRRRVEPG
                     ESGLVKMNAVIARLQERKYLNDPAYAADFARLRQENASFGRRRVQQDLMRKGVQATVI
                     GETLDAAYEDVNEEELARRHLERKRIQKPASQKEAARVARMLMRAGFGSSVIFRILRQ
                     WDIAEDAIAGLESGGEEPGQDAE"
     gene            complement(7462..7881)
                     /locus_tag="VHE33_14560"
     CDS             complement(7462..7881)
                     /locus_tag="VHE33_14560"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HVT98722.1"
                     /translation="MKAVLLDTGVIVALLQRESPWHKECARLVSSLDRPLVTCEAVIT
                     ETCHILRRFPGASEAVMTNLEQGEFEIPFALSQSVPEIRGIMRKYRDLPASFADACLV
                     HMADELDTGDILTLDSDFVTYRWRKNRTFELLIPLKG"
     gene            complement(7878..8114)
                     /locus_tag="VHE33_14565"
     CDS             complement(7878..8114)
                     /locus_tag="VHE33_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98723.1"
                     /translation="MKTTVQARLDEESHAALRQLTRQLGWSPSRVVREGLLLMAQQHG
                     TAHKRKRKIVGLGQFSGGPPDLATNKKYLDDLGR"
     gene            complement(8153..8899)
                     /locus_tag="VHE33_14570"
     CDS             complement(8153..8899)
                     /locus_tag="VHE33_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98724.1"
                     /translation="MAGTAQIPLYSAAKVAQEAQVREAGNGMCWATFGELRAGSDEIE
                     RIVNAVPAVVAAALRQRAWYFVPLAIGENDDTVVAPDYTVDLGDRAICHRNVRFGGSE
                     CIFISTRLMQDRFALAFEFLINVGHHFVDLVGVPQSFADLAWSQATSDVRGETSHDAW
                     ESRRRALDRSRGSEVDERAKSSYLESTFSDALAVYMLSLTVDFDYADLREREYPLLAA
                     PALAERLRHVAEMFPPNTGYEFEIRYRRRG"
     gene            8978..9265
                     /locus_tag="VHE33_14575"
     CDS             8978..9265
                     /locus_tag="VHE33_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98725.1"
                     /translation="MTAATQPARLLGIPLGDFGFFSGLLLALASGFLAFFASCFLAIL
                     ALLFWNLIGHNHINYADSYLYVAFPLGLAVLAFGLVFFTSVWLRRRLSGTN"
     gene            9265..10176
                     /locus_tag="VHE33_14580"
     CDS             9265..10176
                     /locus_tag="VHE33_14580"
                     /inference="COORDINATES: protein motif:HMM:NF022457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HVT98726.1"
                     /translation="MPVVTIPSSGLMPAPEQSRALCAQLEAGDILYFPETPVPLSPDD
                     SAFLLGQQQSDSSLHKNIAYKPNIDRLSGVDTKTADPAAVARLQQIMRGYSTSVVQFL
                     TRFLSPYQQHWKLDYASFRPQEEQNRDLPLRRRNDLLHTDAFPTRPTHGARILRFFNN
                     INPTRTRDWIVSDPFAALVRRFAPQEIAPHPDSPLSRAGKAAGRALGLGAAIPSLKRS
                     PYDDFMMRFHNFLKENPRFQSDCPKYPFQFPPGSSWMVYTDTVPHAVLAGQYALEQTF
                     LVRPEAMVRPEVSPLKVLEEIAQAPLV"
     gene            10265..10759
                     /locus_tag="VHE33_14585"
     CDS             10265..10759
                     /locus_tag="VHE33_14585"
                     /inference="COORDINATES: protein motif:HMM:NF025268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HVT98727.1"
                     /translation="MTSKAPRALLALLAALSLPAFAQFTPRHIYNENADAHAEIREAV
                     ATATRQHKHIILDFGGNWCGDCQVLDIYFHRSPNDALLKSSYVLVDIDIGRMDKNVDV
                     AQKYDVPLKRGVPALAVLDSHGHLLYSQKQGEFESMRRMNPESVTQFLEKWKAPGHDN
                     DGLY"
     gene            10836..11825
                     /locus_tag="VHE33_14590"
     CDS             10836..11825
                     /locus_tag="VHE33_14590"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HVT98728.1"
                     /translation="MLRMLHHLRRAVWAAFGHSVFSMAKAAAYSAMLSIFPALVVITT
                     LFAMMPSGDDIRGVLRASLQQVLPPDTMLLVQNYFINYHTRSIRIVWGCGFVSVFAAM
                     GVLLTFMDGFRRAYRLPRGVWGFWQERLAAAVLVPATILPMAVATGFLTFGHSIETWM
                     IQNSDHVLQFYVILFWRLTRWTISVAASVMALVLIYHFGLPRHKHPLRGGKRAFIRGR
                     WRETLPGAAIATASWFISTLLYGWYVTRFADYSIVYGSLSTAIATLVWLYMTSFSVLI
                     GAEFNAQLFPFPDIRVQDESESDETPDPDLLESDSASNQSILPPHAARSAAAR"
     gene            11911..13473
                     /gene="pyk"
                     /locus_tag="VHE33_14595"
     CDS             11911..13473
                     /gene="pyk"
                     /locus_tag="VHE33_14595"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HVT98729.1"
                     /translation="MPSGTGVASDSLRRAKIVATLGPASSSETIFRELIRAGLDVARL
                     NFSHGTLPEKQKLIEMVRKIAREEKKNVCILGDLQGPKIRTGRLKNRIPVQLKAGQRL
                     TITPRDILGTSTVISTTFPTLAENLEPGARILLSDGLIELRVLEIPGEDVECEVINGG
                     MLGEHKGINLPGIPVRVPSLTDKDEEDLEFGMKHGVDAIAVSFVRTAEDVRHVKFRVA
                     ALGMETWVIAKLEKPQAIENLEAILENADGIMVARGDLGVEMPPEKVPAIQKHVIRRA
                     AEHRKPVITATQMLESMIENPRPTRAEASDVANAIYDGTDAVMLSAETAAGKYPVEAV
                     KMMAKIVSETETNVRDANVWDGRRNYHMRLSIAETICESAAHAAEDLDVSAIAVFTES
                     GTTARQLSKYRPKPPIYALSSIDSVIHRMSLLWGVHPIKCPKLQTSEQMVELAETVLE
                     EGGFVKPKEILGIVAGTQTRSGSTNFLRLHILGDRLTPELPRAGKATQSSKKASRKAT
                     SRKAGKPGRSTR"
     gene            13718..14017
                     /locus_tag="VHE33_14600"
     CDS             13718..14017
                     /locus_tag="VHE33_14600"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HVT98730.1"
                     /translation="MTKQTEEMRALVLQIAKALVDIPDDVNLEVVEKETGSVFRLRVA
                     PEDTGKVIGKQGRTARSIRTILAACSMKYHHHYSLEIIEEQGQAPARSAVLAQAN"
     gene            14233..14475
                     /locus_tag="VHE33_14605"
     CDS             14233..14475
                     /locus_tag="VHE33_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98731.1"
                     /translation="MNTARLMTQLLEIEQAVGKLEKSRILGMLFEAQESVLDLEKQMI
                     EILTDYEGLQMLMEFSRNAAGSTLSQKEIPAKLLVN"
     gene            complement(14572..14799)
                     /locus_tag="VHE33_14610"
     CDS             complement(14572..14799)
                     /locus_tag="VHE33_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98732.1"
                     /translation="MQTEPLIQKLFAIERAIGRVQPAQLRAMVIEAEEAALRMELDNY
                     RSIEELQLRLRERSDEARLGWASSRVLRRTA"
     gene            15018..15293
                     /locus_tag="VHE33_14615"
     CDS             15018..15293
                     /locus_tag="VHE33_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98733.1"
                     /translation="MARVLVVAECEDPKKWRENFVKHVDLFRSMGATAPVSFGLDGNS
                     VAVMLDVKDLNAYQKILQSQATAEAMAMDGLRRETVKAFVLDGQLAV"
     gene            complement(15410..16081)
                     /locus_tag="VHE33_14620"
     CDS             complement(15410..16081)
                     /locus_tag="VHE33_14620"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVT98734.1"
                     /translation="MGKVEAFSDHSGAEPVRGFLHTPELSAGISNALVLSHSAGSNCQ
                     APLLVLLAEAFAGAGIAVLRCDLPFRQERPTGPPLRTAPRDQAGLRAAVEAVRRAVQP
                     THVFLGGHSYGGRMASMVAAESPDLVEGLLLLSYPLHPPKQPQQLRTKHFPALKTPAL
                     FASGTHDGFGLMAEMEAALALIPARTRLVAIAGAGHELLSKTSRATLAETVVGAFLAF
                     TGEGG"
     gene            complement(16084..16437)
                     /locus_tag="VHE33_14625"
     CDS             complement(16084..16437)
                     /locus_tag="VHE33_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98735.1"
                     /translation="MRPFAITVISWLFIALGIVGLVRHGPPAFHSFHQEDVWILLSEV
                     LAIVAGVFMLRGADWARWLAVVWAAAHVAIGWLNGRSEAVIHALIFLGIAMLLFRADA
                     RAYFRPRPADGGAGT"
     gene            complement(16620..17024)
                     /locus_tag="VHE33_14630"
     CDS             complement(16620..17024)
                     /locus_tag="VHE33_14630"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT98736.1"
                     /translation="MTQISPMLAVSDGAAAVEFYQAAFGAEVLWRLDEGVATIAGLTI
                     GGAPFFLAHESPPYGTRSPDGAGFTTVRIELFVDDPVEVQRRAIVAGARLRNEVQKHE
                     HPTEGPKPIRRMLQGSVTDPFGHIWLIGKFLE"
     gene            complement(17021..18169)
                     /locus_tag="VHE33_14635"
     CDS             complement(17021..18169)
                     /locus_tag="VHE33_14635"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HVT98737.1"
                     /translation="MAQNEDYWSVIQRAYSVNPDIVNLNNGGVSPAPIVVQEAVERYN
                     QLSNEGPSYYMWQILDQGREPLRQKLAALAGAKPNEIAIDRNSTEALNTIIYGVPLKA
                     GDEVIGAKQDYPHMIEAYRQRAERDGIVYKQLSFDLPMEDDDAIVRAYEQAITPKTKL
                     IHVTHMINWVGQTMPVAKIADMAHSHNVEVLCDGAHSFGLIDFKVPDLHCDYFGTSLH
                     KFLSAPIGTGMMWIREDHIEKVWPLTCASNPHANDIRKFEDLGTRSLPLEQGIGEAIN
                     FHEGIGAERKQQRIFYLKNYWSTRVQSIPKVKLHTSLKPEYSCAIGGVSIDGMTPQQV
                     ASGLFEKYKIHTVGIVWENISCVRVTPHVYTRTADLDLLVKAIGELAA"
     gene            18602..>19444
                     /locus_tag="VHE33_14640"
     CDS             18602..>19444
                     /locus_tag="VHE33_14640"
                     /inference="COORDINATES: protein motif:HMM:NF015652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HVT98738.1"
                     /translation="MTGTYDSGLVVLSLLMASLASYTALDLAGRVAASRGRAAAWWLA
                     GGSVAMGIGIWSMHFIGMLAFRLPVPMSYDPTITLLSLLIAILSSLFALWIVCQSHLS
                     WPQLAAGALILGAGVCSMHYTGMAAMRMMPPIRYDPVLFSCSVLIAVVASGAALWMAF
                     RLRHKVKGVRRLRAAAAVVMGLAIAGMHYTGMAAARFPANTHCMAVDSGLSSHWLALL
                     ITIFAGSVLSIALIISILDMRLDERTALLAAGLADAKEELKFLALHDSLTKLPNRTLL
                     ADRLE"
BASE COUNT         3812 a         6913 c         5155 g         3564 t
ORIGIN      
        1 aggcacacat cgagaccgat aaagtccgcc agcgtcagcg gtcccatcgg gtgcgccatc
       61 cccagccgga agacctcatc gatcgcatcc ggcgttgcca ccccttccat caccgcgtac
      121 atggcctcgt tcagcagcgg catcagcacc cggttcgata caaatccagg cgcgtcgttc
      181 acctcaaccg ccgtcttgcc cagcttctcg gccagcgtcg cgaccgtctc atacgtctcc
      241 tggctcgtct gcaatccgcg aatcacctcc accagcttca tcaccggcac aggattgaag
      301 aagtgcatgc cgatcacccg ctccggcctt cctgtgacgg ccgccagctt cgtaatcgaa
      361 atcgacgacg tgttcgacgc gagaatcaca tcgggtttgg cgatcgtatc cagttcctgg
      421 aacagcgcct ttttgatctc gaacttttcc gtcgctgctt cgacgatgaa gttcgcttcc
      481 gccaggtccg tccggtccag cgtgccctcg atccgcgcca gcgcatcgtc cacgtcgttg
      541 accgtgatct tgcccttctc cgcctcgcgc cccaggttct tgcggatcgt ctccaggcct
      601 cgatcgagaa accgccgctc cacctcgcac agcaacacgc gaaatccgct gcgcgcgaag
      661 atatgcgcga tgccgttgcc catggtgccg gcgccgatca cgccgaccgt ctgaatctca
      721 ctcatagtgc gttactccgt tttggccacg ctccgcgggc tgatgaacag cagaagccca
      781 aaaccaatag caaagaccag atacaggagc gccaccagca gaataaagcc gctgcgctgc
      841 gcggagacag acagccgcac cagcgctccc cccatcaggc aaaagtgagc gaagttgccc
      901 accgccaccg gccggctgta aatacccccg atagcgacgt cctttgccat ccagttgagc
      961 atcgcgaacc ccgcgtacaa ggcgcccgca gcctgggcca tcagaacagc aaagccgcgc
     1021 ggctcggcgc cgccccaggc cagcacctcc tgtggcgcga aagtgagcgc aaggcccgca
     1081 aagcccatgg cggcggcact ggcgatcatc agaactcgcg tattcatatc gctgactcgc
     1141 tgactcgctg acccgctact ccgccggctc gctgccggct actggctgca cctgcaccac
     1201 cagcgcggtc acgttgtcgc gcccgccggc gtcgttggcc gcatccacca gcgcctgcgc
     1261 aactcgatca agatccctgc ctgcaccgag aatgcgcgca atcccacctt catccacctc
     1321 gcgcgtgagc ccatcgctgc acagcaggaa aacatccccg gcctgcaact cgatctcaga
     1381 cacatcctcc gtgacggagg gctgcgttcc gaccgcccgc gtgatcacac tgcgcaacgg
     1441 agaacgctcc gcttcttcgg ccgtcatggc gcccaaacgc atttgctcgt ccaccagcga
     1501 gtggtcctga gtcacgcgct ccagttttcc cgcgcgcaac aggtagcagc ggctatcgcc
     1561 ggcatgagcg accagagcat ggctgttctc cagcaacagg gccaccagcg tggttcccat
     1621 gccgctcagt tgaggttccc gctcggcacg ttccagcacc aggcggtttg cctccgcaat
     1681 cgcgtcatgc aggccatcgc gccccggcgt tgctgcgggg ttcgcactca tgcggtccag
     1741 aaccgtgtcg accgccatgt ggctcgcgac ctcgccgccg gccgccccgc ccatcccgtc
     1801 gcaaacgacg tagatgccgt gctccgggcg catgccaaac gcgtcttcat tgcctgtgcg
     1861 gactcgtcct ttgctggtac gcgccgctgc cttcgccgtc atcatcaccc gggttgctga
     1921 acctgattca cgatacacga accgctcgat tttctcgtca tcctctcgtc ccacttcatt
     1981 caccggcgcc ggatcatcga atgctgctgg ctactggcca ctggtcactg gctactggct
     2041 attggctact ggctaccggc tcttgtgctc cccgtcacca tcccaccgcg cccccgtcct
     2101 ctacaatcac cctgaggaac tatccaccat gccgcgcaag ccgctcatcg ccgccaactg
     2161 gaaaatgtat aagacccccg cccaggctca ggagttcttc aagaactttt tgcccctggt
     2221 ggccaatcac gaacgcgacg agatcgtcgt ctgcccctcc tacacctcga ttcctgtcgt
     2281 catggccgcg gccacgggtt ccggcgttgc catcggcgcg cagaacatgc actttgccga
     2341 tgaaggcgcc tacaccggcg agatctcgcc cgtcatgctc aaggcgatcg gccccacgca
     2401 cgtcatcctc ggccattccg agcgccgtca gtacttcgcc gagaccgatg agctgatcaa
     2461 taaaaaactc aaggccgccc tccagcatca gctcatcccc atcgtctgcg tcggcgaagt
     2521 gctcgccgag cgcgaagccg gcaaaaccaa tgaagtcctg gtgcgtcaga cgcgcggcgt
     2581 cctcgccgga atcaccgcag ccgacgccgc tcccatcgtc atcgcctacg agcccgtctg
     2641 ggccatcggt accggcaaaa ccgccacccc gcagatcgcc tcggaagccc atgccgtgat
     2701 tcgcgccgaa gtggcaaagc ttctcggtca ggaagcagcc gacgccatcc gcatcctcta
     2761 tggaggcagc gtgaagcccg acaactcccc ggccctgatg gccgaagctg aaatcgacgg
     2821 ggccctggtc ggaggcgcaa gcctcgaccc gcagtccttc gcaaaaatcg tcaattacta
     2881 gctgtcgcgc atcgaacaga ggatgaacga accggcaacg atccgcgaaa tcctggaaaa
     2941 cacccgtacc atcgccgtgg tcggtctcac caatcgcgag ggccgagcct ccctcggcgt
     3001 ctcgcgcttc atgcagtccc agggctatcg catcatcccc gtcaacccac aggtggagga
     3061 agctctcggg gaaaaagcct atccaaccct tgacgacgcc gtcgccgccc atcctgaaat
     3121 cgacctggtg aatgtctttc gccggcccga gcacgtgccc gagatcgtta aggatgcgat
     3181 ccgcctcaag attccccgcc tctggctcca ggaaggcgtt tgccacgacg aagccgtcgg
     3241 atgggccgag gccgcaggaa ttaaagtcgt ccaggaccat tgcattctca aggaacgcat
     3301 ggccgcaggt tggcgtggcc gtagcgaatc cacgattgga cggtcctaat ccacagcacc
     3361 ggcacctggc atagaaaaaa gcgcagcctc tgccgataag gtaggcgtgc agcctccggt
     3421 cccaatcgat ttttggcagg gaagaatcga acaggcctgc cggttcggtt catcgccggc
     3481 gtgggcggcc acggataccg caacaccctt gccaacccag atcgagcccc tgctcggaca
     3541 aaacgccgaa cccgacccga ccttctttgt cgagagctgg acgatcggag ttaccggagc
     3601 ctctgaaaat tatctccgcc gtcatcaaca cgagaaggac caccccgctc cggctcccat
     3661 gtctttcccc agctactcct ttgtgacatt cctgaatgtc gatccgcaca cccccatccc
     3721 aacccagtcg tcaccgaaga cgtcgatgtc ggacggccat tcttttgcca gacgcacgcg
     3781 cagtgaagaa gacaacgaag ccttcgtgcc cggcccgctc acgttccagt ccgcctgtcg
     3841 cctgctggga gtcacagcaa cgagcacccg agacgaaatc cgcgccgcct accgcaaaat
     3901 ggcgagccgc tatcatcccg accggctggc cgctgccggc gtcggagact cacaattcgc
     3961 cagcgatcgg atggcctcga tcaatgaagc ctaccgcctg ctctgcgact ccgctgttgc
     4021 gcaatcagcg ttaaggtaac cgcctcaatc gcgaaaccgt cctgctactc cgtccgcacg
     4081 acctcatact ccatccgcag cgtgcccgtc ttcggatata cgcgatgacc cagcagcttc
     4141 agcttcgttg gcccggcctt cgcaggcacc atcggaattc cggcaccgag cagcgccgca
     4201 atgacagaaa cctcgatgcg atccaccaaa cccgccgcca gaaaactccc cagcaactcg
     4261 cctccaccga agagccacaa gtcctttcca cgacgcgagc gcaaatcgcc gaccacgctc
     4321 cccggatcgc tctcgatccg aacccgcgga aattccgcag cgcgcagcgt gcgagagaac
     4381 acaacatact cgacgccctt cggcggttgg tcccgctttg ccttcaccat cagttcaaac
     4441 gtcttccggc cgatgagaaa cgtgtcgaac tccgccatca tcgctgaaaa atcaacgtcc
     4501 ggatcgtgca cgatccagtc ggactccccc ttcggtccgg cgataaaacc atccaggcta
     4561 acggcggcag agaatcggac gaggcgcatg acaatctctc gacccggccc taaacgccca
     4621 cacccttcgc cttcacgttc tcccgaataa acctcaccac cgactccagc gaagtccccg
     4681 gtccaaacac cgccgacacg ccgctttcct tcagcgccgc aatatcctgt tccggaatcg
     4741 tgcccccgag cacaaccaga atgtccgccg caccctgctc gcgcagcatc gccgtcacac
     4801 gcggaacgat ggcgttgtgc gcgccagaaa ggatgctcag gcctacgcag tccacgtctt
     4861 cctggatcgc cgcgctcacg atcatctccg gcgtctggcg aaggccggta taaatcactt
     4921 ccatccccgc atcgcgtagc gcgcgcgcaa tcaccttggc gccgcgatca tgcccgtcga
     4981 ggcccggctt ggccaccagc acacggatgg gatgggttcg ctcagtcatg tggaacgctg
     5041 aagaatacca cgcaaccccc gccctcaggg cacttcaacc gggtgcgcgc ggccgaggcc
     5101 cagccggtgc agcagggtgc tcatcgtcag cccctgcacc acgatcgaaa agatcaccac
     5161 aatataggtg atcgccagca gccgatcgtg cggcctcccc tgcgggaccg acagagccag
     5221 agccacggcc agcccgccgc gcaatccgcc ccacgtcagc acgctcatcg ccccccgcac
     5281 tggcttgtga aaggcgcgaa ccatcccgag agacgcgccg acgctcagcc atcgcgcccc
     5341 cagtaccacc ggaatcgcga gcagccccgc gtacacgtac tcgatgttcc acggcatcgc
     5401 cagcagttcc agcccgagga gcagaaagag gatcacgttc atgaacgcat cgatcagatc
     5461 ccagaaatcg tcgacatgac cgcgcgtctc ctcggacatg cccagatgcc gtgcccgccc
     5521 accaaggacc agcccggctg ccaccgcttc gagcggggcc gagagccgca gcgcgtcggc
     5581 cagagcatat ccccccatcg ccagcgccag ggtgagcatc acttcgacgc gataactatc
     5641 cacggtgcgg atcagccggt agaccatgta tccaagcgcc agcccgagaa tcacgccgcc
     5701 gcctgccttg atcagcagca gccaggtgaa actggcgacc gatggccggc ctcctccgtt
     5761 gatcgtgtca atcagcgtga ggaagaccac cgcgcccacc ccatcgttga acagtgactc
     5821 gcccgtaagc tgcgcttcca gactggctgg tgcatccaca cgccgcagca gttccagcac
     5881 cgcaatcgga tcggtcggcg agatcagcgc cccaaagagc agacacgcaa tccaggtggc
     5941 gtgaaagccg gttgccgtca ggatcagctt cagcagcgcg gccacaatcg ccgtagaaag
     6001 cagcgtgcca aaaaccgata aggccgtgac cgatgccttc tgcttagcca gttccttcag
     6061 gtcgagtttg agcgcgccgg caaagagcag aaacgcgagc atgccgtgca gcacaaccgc
     6121 ggaaaaatca atgcgcgcca cttccgcggc aaccgtggca tgcaggccgg ggaacgcacg
     6181 ccccacaaac acgaacacca gagacatgcc cagggactgc agcatcacgc caaccgtcac
     6241 cggcaggtgc agcacacgcc ggctcaccag gctgaacgct gcagccaggc agaccagcgt
     6301 gctcaccagc gcgaaaaccg gcatgattcg actctatcgc gccgagagct cgatgaaagc
     6361 cccaatcgtc cgcactcgtt cttcgagcgg tgcggcttcg accgtcaccg gctcgaagcc
     6421 ccactcgcga tagacctctt cgtgaatgcg ctcaaaccga accgtctctt caaacgtgat
     6481 gcgtcgcgcc gccgtcggtt caatgaaccc gaggttctgc agaaaaaaga cactgcgttc
     6541 gtagacgcat tcttcgcgca ctcgctcagc ttcgcggatc agctcggcag gacagggaac
     6601 cgcaagatac ttcgccagtg ccagcgtgca aaagatcgat cgatcgtgaa actgaacggc
     6661 atcgccgctc ttcgatgccc gctccagccg cagcctctgc aaccaagcga catcctccac
     6721 gaatcccgcc tgcatccacg gttccgccat gccctccgcc tggcgcagcg caatcagatc
     6781 ggtagccgcc tcttcgacga cgccgaatcc atcgagttcc agttgccgca gaaccgcagt
     6841 cttgccggat ccgggcgcgc ccgtgaggat atatcgcttc atgctgacgg atcgttttaa
     6901 ggggatttac tccgcgtcct gccccggctc ctcgccgccc gattcaagtc cggcgatcgc
     6961 gtcctctgcg atatcccatt ggcgcaggat gcgaaaaata acactggacc caaatccggc
     7021 gcgcatcagc atccgggcca cccgcgccgc ctccttttgg ctggccggct tctggatgcg
     7081 cttgcgctcg aggtggcggc gagccagctc ttcctcattc acatcttcgt aggccgcatc
     7141 gagagtctcg ccgatgactg tggcctgcac gcccttccgc atcaggtcct gctgcacccg
     7201 ccgtctgccg aagctggcgt tttcctggcg cagcctcgca aagtccgccg cgtaggcagg
     7261 atcgttcagg tacttccgct cctgcaggcg tgcaatgacc gcgttcatct tcacgagccc
     7321 ggattcgccc ggctcaaccc gccgtcggag caagcgcttc acttcggcga ccgtccgcat
     7381 ctgctggccg agaagcttca ctgcatagtc gaacagctcg ttttcattca ggggtgcggc
     7441 aggcttacgg gcgcgcatgg actagccctt cagcgggatc agcagttcaa aagttcggtt
     7501 tttgcgccag cgatatgtaa cgaaatcact gtccaaagtc agaatatcgc cggtatcaag
     7561 ctcatctgcc atatgaacaa gacacgcatc ggcaaagctt gccggaagat cgcgatactt
     7621 acgcatgatg cctcgaattt ccgggaccga ctgcgacaga gcaaacggga tttcaaattc
     7681 cccctgctcg agattcgtca tgactgcttc tgaggcgccc ggaaatcttc ttaaaatatg
     7741 acaggtttcg gtgatcactg cttcacaggt gaccagagga cggtccagac tcgaaaccag
     7801 acgcgcacat tctttatgcc atggcgattc tcgctgcagc agcgcgacaa tcacacccgt
     7861 atcaagaagg accgctttca ccggccaaga tcgtccaggt atttcttatt cgtggcgagg
     7921 tcgggcggac ctcctgaaaa ctgtccgagc ccaacaatct tccttttcct cttgtgagca
     7981 gtgccgtgct gttgcgccat cagcagcagt ccctcgcgca ccacgcggga agggctccag
     8041 cccagctggc gggtcagttg ccgcagggca gcatgggatt cctcatccag tcgtgcctgc
     8101 accgtggtct tcatagcacg attgtattac aaaacgtcag ttgtaataca gactagcccc
     8161 gccggcggta ccgaatctca aattcgtaac cggtattcgg cgggaacatc tccgccacat
     8221 gccgcagccg ttccgccagc gccggcgccg ccagcagcgg atactcccgc tcccgcaaat
     8281 cggcgtagtc gaagtccacc gtcagcgaaa gcatatagac cgccagcgca tcggagaacg
     8341 tcgattccag atagctcgat ttcgcccgct catccacctc gctgccccgg ctgcgatcga
     8401 gcgcccgtcg ccggctctcc catgcatcgt ggctggtttc gccgcgcaca tcgctcgtcg
     8461 cctgcgacca cgccagatcg gcaaagctct gcggcacgcc aacgagatcc acgaaatggt
     8521 gccccacatt gatcagaaat tcgaaggcca gcgcgaaccg gtcctgcatc agccgggtcg
     8581 agataaaaat gcactccgaa ccgccgaagc gcacgttgcg atggcaaatg gcgcgatcgc
     8641 ccaggtccac cgtgtagtcc ggagcgacca ccgtgtcgtc gttctctcca atcgcaagcg
     8701 gcacaaagta ccacgcgcgc tggcgaagcg cagcagcgac gaccgccggc acggcattca
     8761 cgatgcgctc gatctcatcg ctgcccgcgc gcagttcgcc gaacgtcgcc cagcacatcc
     8821 cgtttccggc ctcgcgcacc tgggcctcct gggccacctt cgctgcggaa tacaagggga
     8881 tttgcgcggt tccagccata agtctgtatt ctagtggcgt gctgctgaaa tacctaattg
     8941 cacccgcttt ctccagcttc aacagggaac ctgcgcgatg accgccgcca cccaaccggc
     9001 ccgcctgctg ggcattcccc tgggcgattt tggcttcttc tcggggcttc tcctcgccct
     9061 cgcctccggg ttcctcgcct tctttgcctc ctgtttcctc gccatccttg cgctcctgtt
     9121 ctggaacctc atcggccaca accacattaa ctacgcggac agctacctct acgtcgcttt
     9181 cccgctcggc ctcgccgtcc tggccttcgg cctcgtcttc ttcacctccg tgtggctgcg
     9241 ccgccggctc tcaggaacga actgatgccc gtcgtcacga ttccctccag cggcctgatg
     9301 cccgcgcccg agcagtcccg agccctctgc gcccagcttg aggccggcga catcctctac
     9361 ttccccgaga cgcccgtacc cctctccccc gacgactcgg cctttctcct cggccagcag
     9421 cagtccgaca gttcgctgca caagaacatc gcctacaagc ccaacatcga tcgcctcagt
     9481 ggcgtcgata cgaagaccgc cgatcctgcc gcggtcgccc gcctgcagca gatcatgcgc
     9541 ggctattcca cctcagtcgt ccagttcctc acccgtttcc tgtcgccata tcagcagcac
     9601 tggaagctcg actatgccag ctttcgtccc caggaggagc agaaccgcga tctacccctg
     9661 cgccgccgca acgacctgct ccacaccgat gcctttccca cccggcccac ccacggcgcg
     9721 cgcattctgc gcttcttcaa caacatcaat ccgacccgca cccgcgactg gatcgtgagc
     9781 gacccctttg ccgccctggt tcgcaggttc gcaccccagg agatcgctcc gcatccggac
     9841 tccccgctct cgcgcgccgg aaaagccgcg ggccgggcgc tcggtctcgg cgcagccatc
     9901 ccctcgctga agcgctcccc ctatgacgac ttcatgatgc gcttccacaa cttcctgaag
     9961 gaaaatccgc gcttccagtc cgactgtccg aagtacccgt ttcagttccc acccggctcc
    10021 tcgtggatgg tctacaccga tacggtcccc catgcagtcc tcgccggcca gtacgccctc
    10081 gagcaaacgt tcctggtacg gcccgaagcc atggtccgcc ccgaagtctc cccgctgaaa
    10141 gtgctcgagg agatcgctca ggcccccttg gtctgacttt ctgcgcctcc cgccatcctg
    10201 tggcatccta atatccacag gagccacagt tcctgcagga ccccagtccc tggcgatgaa
    10261 atccatgacc tccaaagctc cccgagccct gctcgctctc ctggccgcac tttcgctgcc
    10321 cgccttcgcc cagttcaccc cccggcacat ttacaacgag aatgccgacg ctcacgccga
    10381 gattcgcgaa gcagtggcga cggccacgcg ccagcataag cacatcattc tcgacttcgg
    10441 tggaaactgg tgcggcgact gccaggtcct ggacatctac ttccatcgct cgcccaatga
    10501 cgcgctgctg aaatcgagct acgtcctggt cgacatcgac atcggccgta tggacaagaa
    10561 cgtcgacgtg gcgcagaagt acgatgtgcc gctgaagcgt ggcgttcccg ccctcgccgt
    10621 gctcgactcg catggacacc tgctctatag ccagaagcag ggcgagttcg aatccatgcg
    10681 gcgcatgaac ccggagtccg tgacccagtt cctcgaaaag tggaaggcgc ccggacacga
    10741 taacgacggt ctctactaac caccacggtc gaatccgtcg gtccggcccc ttttccatct
    10801 gggccatgca gtcgcgacgt taaactgaac tccgtatgct gcgcatgctc caccatctcc
    10861 gccgcgccgt gtgggccgcg ttcggccaca gtgtgttctc catggccaag gccgcagctt
    10921 actccgctat gctcagcatt tttcctgccc tggtcgtcat caccacgctt tttgcgatga
    10981 tgccttccgg cgacgacatt cgcggcgtgc tgcgcgcctc ccttcagcag gtgctgcccc
    11041 ccgacaccat gctgctggtg cagaactatt tcatcaacta ccacacccgc tccatccgca
    11101 tcgtctgggg ctgcggcttc gtcagcgtct ttgccgccat gggtgtcctg ctcaccttca
    11161 tggatggttt ccgccgcgcc tatcgcctgc cgcgcggtgt gtggggtttc tggcaggaac
    11221 gcctcgccgc ggcggtcctt gtccccgcca ccatcctccc catggccgtc gccacaggat
    11281 tcctcacctt cggccactcc attgaaacct ggatgattca gaactccgac cacgttctgc
    11341 agttctacgt catcctcttc tggcggctca cccgctggac catctcggtg gccgccagcg
    11401 tcatggcgct cgtgctcatc taccatttcg gcctgccacg ccacaaacac ccgctgcgcg
    11461 gcggcaaaag agctttcatc cgcgggcgct ggcgcgagac cctgcccggt gcggccatcg
    11521 ccaccgcttc ctggttcatt tccaccttgc tgtatggctg gtacgtcacg cgcttcgccg
    11581 actactccat cgtctatgga tcgctcagca ccgccatcgc caccctggtc tggctctata
    11641 tgacctcctt cagcgtcctc attggcgctg agtttaacgc tcagcttttc cccttccccg
    11701 acatccgcgt tcaggatgaa agcgaatccg acgaaactcc cgatcccgat ctgctcgagt
    11761 cagactccgc ctcaaatcag tcaatccttc ccccacacgc tgcccgttcc gccgccgcac
    11821 ggtaaaatcg gttcgggact cgaaccattt cccgggcgcc ctctccagaa cgcgtcctca
    11881 gatcacattt ttgggggttg gatgaacttc ttgccgtcag ggacgggcgt cgccagcgac
    11941 agcctgcgcc gtgccaaaat cgtcgccaca ctcggaccag cttcttccag tgaaaccatc
    12001 ttccgcgaac tgatccgcgc cggcctcgat gtcgcccgcc tgaatttctc gcatggcacc
    12061 ctgcccgaaa agcagaagct catcgaaatg gttcgcaaaa tcgctcgcga agaaaagaag
    12121 aacgtctgta tcctcggcga tctgcaaggg cccaagattc gcaccggccg cctcaagaac
    12181 cgcattcccg tccagctgaa ggccggccag cgcctcacca ttaccccccg cgacatcctc
    12241 ggcacctcca ccgtgatctc caccaccttt cccactctgg ctgagaatct cgagcccggc
    12301 gcgcgcattc tcctctctga cggcctcatc gaactgcgtg tgctcgaaat tcccggtgaa
    12361 gatgtcgagt gcgaggtcat caacggcggc atgctcggcg aacacaaagg catcaacctg
    12421 cccggcatcc cggtccgcgt tccttccctg accgacaagg acgaggaaga tctggaattc
    12481 ggcatgaaac atggcgtcga cgccatcgcc gtctccttcg tccgcaccgc cgaagacgtc
    12541 cgccacgtca aatttcgcgt cgccgccctc ggcatggaga cctgggttat cgccaagctg
    12601 gaaaaaccgc aggcgatcga aaatctcgag gcgatcctcg aaaacgccga cggcatcatg
    12661 gtcgctcgcg gcgacctggg cgtcgagatg ccgcccgaga aagtcccggc catccagaag
    12721 cacgtcatcc gccgtgccgc cgagcaccgt aagcccgtca tcaccgcgac gcagatgctc
    12781 gagtccatga tcgagaatcc ccgccccacc cgcgccgaag ccagcgacgt cgccaacgcc
    12841 atctacgacg gcaccgacgc cgttatgctc tcggccgaaa cagccgccgg caagtatccc
    12901 gtcgaagccg tcaagatgat ggccaagatt gtcagcgaaa cagaaaccaa tgtccgcgat
    12961 gccaacgtct gggatggccg ccgcaactat cacatgcggc tctccattgc cgaaaccatt
    13021 tgcgaatcgg ctgcgcacgc cgccgaggat ctcgatgtca gcgccatcgc cgtctttacc
    13081 gagtccggca ccaccgcgcg ccagctgtcg aagtaccggc ccaagccgcc gatctatgcc
    13141 ctctccagca tcgactccgt catccatcgc atgtccctgc tttggggcgt ccatcccatc
    13201 aagtgcccca agctgcaaac ctccgagcag atggtcgagc tggccgaaac cgttctggag
    13261 gaaggcggct tcgtcaagcc gaaggagatt ctgggcatcg tggccggaac tcagacccgc
    13321 tccggctcta ccaacttcct ccggctgcac atcctcggcg accggctgac cccggagctt
    13381 cctcgagccg gcaaagccac ccagtcctcc aaaaaagcaa gccggaaagc cacctcgcgg
    13441 aaagcgggaa agcccgggcg ctccacaagg tagggggcgg cgcccttcca gcctgtccca
    13501 ttccgggacc ttcgggctca ttccccgtcg ccgccccgtc gctcccctga atttccctct
    13561 tgcgcaacgg ctgcccgggg cggatattca tctcagcggt aaaactgaaa ccggtactgc
    13621 tccgcggagt tttcaacgcc accgttttgt gggtcgcgaa tctccgtctg ttcccacccc
    13681 gatttcaggg ctccggcgcg gtggtggtgt tatgcagatg acaaaacaaa cagaagaaat
    13741 gcgggctctt gttctccaaa tcgcgaaggc tttagtcgat attcctgacg acgtgaatct
    13801 cgaggtagtg gagaaggaga ccgggtcggt ctttcgcctc cgcgtcgctc ctgaagatac
    13861 ggggaaggta atcggcaagc agggccgaac cgcgcggtcg atccgcacga tcctcgccgc
    13921 ctgcagcatg aaataccatc accactattc cctggagatc atcgaggaac aggggcaggc
    13981 tccggctcgc tccgcggtcc tcgcccaggc taactaagca atcgcatcgg tcttgccgtt
    14041 ccgcgcttct ggctggagtg cggacccgag ggtggagtgt gtctgctcct ggcactcgac
    14101 cggtccggcg gacggaccct ccaggacctg gatgcgccct cctatacccc ctgcaagagt
    14161 gatttgcatc acatccctcc ccagcctcac ccggcaaact acgggaacac tcgcggtaag
    14221 gagcccgact cgatgaatac cgctcgcctg atgacccagt tgctcgaaat cgaacaagcc
    14281 gtcggaaaac tcgaaaaatc ccgcatcctc ggcatgctct ttgaggccca ggagagcgtc
    14341 ctcgatctcg aaaagcagat gatcgaaatc ctcaccgact acgaagggct ccagatgctc
    14401 atggagttca gccgcaacgc agcgggttca acgctgtcgc agaaggaaat ccccgcaaag
    14461 ctccttgtga attaggacgc cgctccaggg ggaatgaagc gacgtccagg agaacggacc
    14521 tgaaacccag ggcccggcta taacatcttt gaatgtggct atccggcagc cttaggccgt
    14581 tcgtcgcaga acgcgcgatg acgcccatcc gaggcgagcc tcgtcggaac gctctctcaa
    14641 acgcagttgc aattcttcaa tcgagcggta gttatccagc tccatccgca aagcggcctc
    14701 ttcagcctcg atcaccatcg cccgtaactg cgcgggctgg actcggccaa tggcacgctc
    14761 aatcgcgaat aacttctgaa tcagaggttc ggtttgcatc tcgggcagat cctgaaagta
    14821 ctgcaatcgg tgacgggcca cggtcgagct ggggagccca tgcaccagag cacatcctgg
    14881 tggagattga aactcaacga cattccacac ccgggttatt accaagggtg ttaacgaacc
    14941 aaataactgg ttgcgccgcg ccgcgcgccc tgccagaatg gcttgctccc ccgagcccac
    15001 gaaacggaag gaaagacatg gcccgagttc tggttgtagc cgagtgcgag gacccgaaga
    15061 agtggcggga aaactttgtg aagcatgtcg acctgttccg cagcatgggc gcgacagcgc
    15121 ccgtcagctt cggactggac ggtaattcgg ttgccgtcat gctcgatgtg aaagatctga
    15181 acgcctatca gaagatactg cagtcacagg ccaccgccga agccatggcg atggatggcc
    15241 ttcgccgcga aacagtgaaa gcgttcgtgc tcgacggtca gctcgcggtc tagtgtgggg
    15301 tttgtgaagt tccttcccct tgtcattccg accggaccct gagcgtcagc gaaggggaag
    15361 cggagggacc tgcttctctt tcgcataaat aagtttcaga aacaccacat tagccgcctt
    15421 ccccggtgaa agccaggaac gcccccacca ccgtctccgc cagggtcgcc cggctggtct
    15481 tgctcagcaa ttcgtgccct gcccccgcta tcgccaccag ccgcgtgcgc gccgggatca
    15541 gcgcaagcgc cgcctccatc tccgccatca acccgaagcc gtcgtgcgtt cctgatgcga
    15601 acagcgccgg cgtcttcagc gcagggaagt gcttcgtccg aagctgctgc ggctgcttcg
    15661 gcgggtgcag cgggtaagaa agcagcaaca gcccttcgac cagatccggt gattcggccg
    15721 ccaccatcga tgccatgcgt cccccatagg agtgcccccc cagaaacaca tgcgtcggct
    15781 gaacagcgcg gcgcaccgcc tccaccgccg cgcgaagtcc cgcctgatcc cgcggagccg
    15841 tgcgcagcgg agggcccgtc ggccgctcct gccgaaacgg cagatcgcag cgcaaaaccg
    15901 caatccccgc cccggcgaac gcctccgcca gcagaaccag cagcggcgcc tggcaattcg
    15961 accccgccga atggctcaac acaagcgcat tcgaaatccc cgcagacaac tccggcgtgt
    16021 gcaggaaccc gcgcaccggc tccgcccccg aatgatcaga gaaagcctcg accttgccca
    16081 tgcttaggtg cccgcgcccc cgtcagccgg cctcggccgg aaatacgcgc gcgcatcggc
    16141 ccggaagagc agcatcgcga tgcccagaaa gataagtgcg tggatcaccg cctcgctgcg
    16201 tccattgagc cagccgatgg ccacatgggc ggcagcccag accacggcca gccaccgcgc
    16261 ccagtcagcc ccgcgcagca taaagacgcc cgcgacgatc gccaacactt cgctgagaag
    16321 gatccagaca tcttcctggt ggaaagagtg aaacgccggc ggcccatgac gaaccagccc
    16381 aacgataccg agagcaataa aaagccagct gataacggtg atggcgaatg ggcgcatgct
    16441 gttcaatgaa accggtggcg ttcctgaaag ttccttctct gtcatcccga ccgaaccctg
    16501 agcgtagcga aggggaagcg gagggacctg ctttcaccct gtcatcccga ccggaccctg
    16561 agcgcagcga agggggagcg gagggacctg ctttcccctt ttgtcagcca gcgtgctggc
    16621 tattcaagaa acttcccgat cagccagata tgcccaaacg gatcggtcac cgatccctgc
    16681 agcatccggc gaatcggttt cggcccttcc gtcgggtgtt cgtgcttctg cacctcgttc
    16741 cgcagtcgcg cccccgcgac aatcgcgcgg cgctgcacct caaccggatc gtccacgaat
    16801 aactcgatgc gaaccgtcgt gaatccggct ccgtccggac tgcgcgtccc atagggcggc
    16861 gattcgtgcg ccaggaaaaa tggagctcct ccaatggtca gccccgcgat cgtcgcaacg
    16921 ccttcatcga gacgccaaag aacctctgcg ccgaacgccg cctggtaaaa ctccaccgcg
    16981 gcagcgccgt ccgacaccgc cagcatcgga gaaatttgcg tcatgccgcc agctcaccga
    17041 tcgctttcac cagcagatcc agatccgccg tccgcgtgta cacatgcggc gtcacccgca
    17101 cgcagctgat gttctcccac acaatgccga ccgtgtggat cttgtacttc tcgaacagcc
    17161 cgcttgccac ctgctgcggc gtcatgccat cgatcgaaac cccgccgatc gcgcacgagt
    17221 actccggctt cagtgacgtg tgcagcttca ccttcggtat cgactgcacc cgcgttgacc
    17281 agtagttctt cagatagaag atgcgctgct gcttccgctc cgccccgatg ccctcgtgga
    17341 aattaatcgc ctccccgatc ccttgctcca gcggcaaact ccgcgtcccc agatcttcga
    17401 acttgcggat gtcgttggcg tgcggattcg acgcgcaggt cagcggccac accttctcga
    17461 tgtgatcttc ccgaatccac atcatccccg tcccgatcgg tgccgacaaa aacttgtgca
    17521 ggctggtgcc gaagtaatcg cagtgcaggt ccggcacctt gaagtcgatc agcccaaacg
    17581 agtgcgcccc gtcgcaaagc acctccacgt tgtggctgtg cgccatgtcc gcgatctttg
    17641 ccaccggcat ggtctgcccg acccagttga tcatgtgcgt cacgtggatc agcttcgtct
    17701 tcggcgtgat cgcctgctcg tatgcgcgca caattgcgtc gtcgtcttcc atcggaagat
    17761 cgaacgagag ctgcttgtac acgatcccgt cgcgctcggc acgctggcgg tacgcctcaa
    17821 tcatgtgcgg gtagtcctgc ttggcgccga tcacctcatc gccagccttc aacggcacgc
    17881 cataaatgat cgtgttcagc gcttcggtcg aattccggtc gatggcaatc tcgttcggct
    17941 tggctccggc cagcgccgcc agtttctgcc gcagcggctc gcgcccctga tcgagaatct
    18001 gccacatgta ataggaaggg ccttcgttcg aaagctggtt gtagcgctcc accgcttcct
    18061 gcaccacaat gggcgccggc gaaaccccgc cgttgttcag gttcacaatg tccggattca
    18121 ccgaatacgc ccgctggatg acagaccagt aatcctcgtt ctgtgccaca gcggccgggg
    18181 caaggtcccg aaccgggtga gcggccgcgc taaactccgc agcgtgtgcc tgtcgaaaca
    18241 ggctgttaac tccaaaggca cccgcgagcg tggcggtccg tctcagaaaa ctgcggcggt
    18301 cagtcggtgt catggctgcc aatgtaggcg caaacagccc agagaggcaa atcctgtggt
    18361 ccgaacccat ggaccctccg ccagtctgcg cccggcgagt agtcccacaa actcccgggg
    18421 taatgccccg gaagtgggaa atgaaatcct gtacattcgc tgttactctt cccgggttag
    18481 taacgaatcc ggcaaccgca ggcacggagc ggccgccctg atcgaggtat aacgtgccaa
    18541 ccgccttacc ggggccccgc acgtttgaga agtcgacatc ccggtgtgag gacactacag
    18601 cgtgaccgga acctacgatt ccggcctggt agtgctgtcc ctgctgatgg cgtcgttggc
    18661 gtcgtatacc gccctcgacc tggccggacg cgtcgcagcc tcccgcggac gggccgccgc
    18721 ctggtggctg gccggtggtt ccgtcgccat gggcatcggt atctggtcga tgcacttcat
    18781 cgggatgctg gcctttcgcc tccccgtccc catgagctac gacccaacca tcaccctgct
    18841 gtcgctgctc atcgccatcc tttcttctct gttcgctctc tggatcgtct gccagagcca
    18901 cttgtcgtgg ccgcaactgg ccgctggagc gctcatcctc ggggccggcg tttgctccat
    18961 gcattacacc ggcatggctg ccatgcggat gatgccgccg atccgctacg accccgtcct
    19021 cttttcctgc tccgtactga ttgcggtcgt ggcctccggc gcagcgttgt ggatggcttt
    19081 ccgtcttcgc cacaaagtca aaggagtccg ccgcctccgt gccgccgccg ccgttgtcat
    19141 gggcctcgcc atcgccggca tgcactacac gggcatggcc gcggcccgct ttcctgccaa
    19201 tacccactgc atggctgtgg actccggact ctcatcccac tggctggcgc tgctgatcac
    19261 catctttgcc ggctcggtgt tgtccatcgc tctcatcatc tccatcctcg acatgcgcct
    19321 cgacgagcgc accgccctgc tggcagccgg cctcgccgac gccaaagagg aactgaagtt
    19381 cctcgccctc cacgacagcc ttaccaagct ccccaatcgc accctgctgg ccgatcgtct
    19441 cgag
//
LOCUS       DASZYN010000564         3462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1053408, whole genome shotgun sequence.
ACCESSION   DASZYN010000564 DASZYN010000000
VERSION     DASZYN010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053408"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(321..746)
                     /gene="rpsK"
                     /locus_tag="VHE33_14645"
     CDS             complement(321..746)
                     /gene="rpsK"
                     /locus_tag="VHE33_14645"
                     /inference="COORDINATES: protein motif:HMM:NF003698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HVT98739.1"
                     /translation="MADQKPAKGATGKAARNKKFKKRERKNVPFGLVHIQASFNNTIV
                     TITDTQGNTLSWKSSGSLGFRGSRKGTPFAAQQAAMNAANMARDHGVRSVDVRVSGPG
                     SGRESAVRALAAAGIDVRSIRDVTPIPHNGCRPPKRRRV"
     gene            complement(746..1132)
                     /gene="rpsM"
                     /locus_tag="VHE33_14650"
     CDS             complement(746..1132)
                     /gene="rpsM"
                     /locus_tag="VHE33_14650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HVT98740.1"
                     /translation="MARIAGVDLPRNKQARIGLTYIYGIGNSRSLKILSAANVDPFRK
                     IQDLGEDEVNRIRQVIEEQGDVEGDLRKDTAMHIKRLVDIQSYRGHRHRRGLPVRGQR
                     THTNARTRKGPRKGTVANKKKASAKT"
     gene            complement(1375..1488)
                     /gene="rpmJ"
                     /locus_tag="VHE33_14655"
     CDS             complement(1375..1488)
                     /gene="rpmJ"
                     /locus_tag="VHE33_14655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HVT98741.1"
                     /translation="MKVRASVKKICDKCKVIHRRGVVRVICENQKHKQRQG"
     gene            complement(1579..1803)
                     /gene="infA"
                     /locus_tag="VHE33_14660"
     CDS             complement(1579..1803)
                     /gene="infA"
                     /locus_tag="VHE33_14660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HVT98742.1"
                     /translation="MSKEDAIEVMATVLETLPNAMFKVKLESSGGHEVLAHVSGRMRK
                     NFIRILPGDRVAVELSPYDLNRGRIVYRYK"
     gene            complement(1825..2556)
                     /locus_tag="VHE33_14665"
     CDS             complement(1825..2556)
                     /locus_tag="VHE33_14665"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HVT98743.1"
                     /translation="MSTAVADVRVDVKESLKGKPGPILLLGAPGVGKGTQAKELVAKW
                     GIPQISTGDLLRYNRDHGTSLGLKAKEIMAAGKLVPDELVNAMVADRLKQPDTANGYI
                     LDGFPRTLGQAKWLDEHLGAGTLPVIAVSISVGYNELLRRITGRRSCPQCGAIYNIYF
                     QPPKVDETCDLDGTPLVRRSDDTEEVFRERMRTYDSQTAPVVEHYRALGRFEEVNGDQ
                     PVSAVAAGVEAAVARLRNSSTKATK"
     gene            complement(2556..>3462)
                     /locus_tag="VHE33_14670"
     CDS             complement(2556..>3462)
                     /locus_tag="VHE33_14670"
                     /inference="COORDINATES: protein motif:HMM:NF012564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HVT98744.1"
                     /translation="FIMWLGEQITERGIGNGMSLLIFAGIVVGLPRGIADLVSTARSE
                     RFGVFTVPLLILLVVAMIAIVAFIVWVERSERRIPVQYAKRIVGRRLMGGQSTHLPLK
                     VNSGGVMPIIFAASVLSAPMLFAQAAFVKNSSWLSKTFDQLRAGEPWYELLYMISIVF
                     FAYFYISIVFRPDDIADNMRKYGGFIPGIRPGRRTSDFVNEILTRITLVGAIYLIIVA
                     IIPQLLISGIHLNHLWLVGSWFESLPSWMTNGMGFNFYFGGTSLLIVVGVAMDTVNQV
                     ESQLIMRHYEGFSPRSGRVRGRRAW"
BASE COUNT          656 a         1101 c          978 g          727 t
ORIGIN      
        1 aaacacttcg cgcccttcag gaacagcttc atgccttctc gccggcacaa ccggcagacg
       61 gctcccgtat aacgtgccaa tgcattctcc ttaattcaga ggccgaccgg tttacgtccg
      121 cggaggactt cgtcccggtc caggaacagc gttcagggca cagcgttcag cgctcagaaa
      181 aacgcatcgc ctgtgcccta tacaccgtag gtcttagttt aacaggagat ccggaccttt
      241 acccagccct gatgtcctgt tggcccagca gccggcgccg cgtcgaactg aacgctgaac
      301 gccgaacgct gaacgcttgt tcagactctg cgccgcttcg gcggacggca gccgttgtgc
      361 ggaatcgggg tgacgtcgcg aatggagcgc acgtcgatgc cggccgcagc cagagcgcgg
      421 actgcggatt cgcggcccga acccggcccg ctgacccgca catccaccga gcgcacgccg
      481 tgatcccgcg ccatgttggc cgcgttcatc gcggcctgct gggcggcaaa cggagtgccc
      541 ttgcgcgacc cgcggaagcc gagcgagccc gaactcttcc acgagagcgt attgccctgg
      601 gtatcggtga tggtcacgat ggtgttgttg aacgaggcct ggatgtgcac cagaccgaac
      661 ggcacattct tgcgctcgcg ctttttgaat ttcttgttgc gcgcggcctt tccggtcgcg
      721 ccctttgctg gtttctggtc ggccattagg tcttcgcgct cgctttcttc ttgttggcta
      781 ccgtgccctt acgcggaccc ttgcgggtgc gggcgttggt gtgcgtacgc tgtccgcgga
      841 ccggcagacc gcgccggtgc cgatgcccgc gatacgactg gatgtcgacg agccgcttga
      901 tgtgcattgc ggtgtccttg cggagatcgc cttccacatc gccctgctct tcgatgacct
      961 ggcggatgcg attgacttcg tcttcgccga gatcctgaat cttcctgaac ggatcgacgt
     1021 tcgcggcgct cagaattttg agcgagcgag aattgccgat gccgtagata taggtcagcc
     1081 cgatccttgc ctgcttgttg cgcggcagat cgacgcctgc gatacgtgcc atacggtttc
     1141 cttcatgttg cttcggccaa cttcccgaag cgggttttcc ggccgaacct cgaaccggag
     1201 ttttgagagc ctgacgttgt cggggttcat cgcggacctg acttcggttc actcgcccca
     1261 gctgccttca cttccctggc agctcagctc ccggatcaag ctggaagccg gaagcgtgaa
     1321 gccaggagcg atgaaaaact gcgtcctgct tcttccttcc tgcttcccgc tctttcagcc
     1381 ctggcgctgc ttatgcttct ggttttcgca gatcacccgc accacaccgc ggcgatggat
     1441 gaccttgcac ttgtcacaaa tcttctttac cgacgcacga accttcataa aaaacagtcc
     1501 tcagttttca gttatcagtt cacagtttcg ttgccggccc gggcctgatc gcactgggga
     1561 ctgacaactg acaactgatc acttatatcg atacacaatg cgtccgcgat tcaaatcgta
     1621 cggactcagt tccacggcga cgcggtctcc gggaagaatc ctgatgaagt tcttccgcat
     1681 gcgacccgac acgtgggcca gaacctcgtg cccaccactg ctctcgagct tcaccttaaa
     1741 catggcattg ggcaacgtct cgagaaccgt tgccatgacc tcaatcgcgt cttccttcga
     1801 caatcgttat ccttaaactc gtctctactt cgttgctttc gtgctggaat tccgcagccg
     1861 cgccaccgct gcttccacgc ccgccgctac tgcgctcacc ggctgatcgc cgttgacttc
     1921 ctcaaaccgt cccagagcac ggtaatgctc gacgaccgga gccgtctgcg aatcataggt
     1981 ccgcatccgc tccctgaaaa cttcctcagt atcgtctgag cgccgcacca gaggtgtgcc
     2041 gtccaggtca cacgtttcat ccaccttcgg cggctgaaag tagatgttgt agatcgcccc
     2101 gcactgcggg caactgcgcc ttccggtaat acggcgcagt aattcattgt atccgacgct
     2161 gatactgacg gcaatcaccg gcagggtccc ggcgccgagg tgttcgtcca gccacttcgc
     2221 ctgtcccaag gtgcggggga acccatccag aatgtagccg ttggcggtgt ccggctgctt
     2281 cagccggtcg gcaaccattg cattcaccag ctcgtccggg accagcttgc cggcggccat
     2341 gatttctttc gccttcagcc ccagggacgt tccatgatcg cggttgtagc gaagcaggtc
     2401 tccggtcgag atctggggga tgccccactt ggcgaccagc tctttggcct gggttccctt
     2461 gccgacaccc ggcgctccca gcagcagaat gggtcccggc tttcccttga gagactcttt
     2521 tacgtccacg cgcacatccg ccactgccgt actcattacc aggcccgcct tccccggacc
     2581 ctcccgcttc tgggggagaa tccctcatag tgccgcatga tcagctgcga ctcgacttga
     2641 ttgacggtgt ccatcgcaac gcccacaacg atcaggagcg atgtgcctcc gaaatagaaa
     2701 ttgaagccca tgccgttggt catccaggag ggcaggctct cgaaccagct cccgaccagc
     2761 cagaggtgat tcaggtggat accggagatg agcaactggg gaatgatggc aacgatgatc
     2821 aggtagattg cgccgaccag ggtgatgcgg gtcagaatct cgttcacgaa atcggacgtg
     2881 cgccgcccgg gacgaatgcc tgggatgaag ccaccgtact tgcgcatgtt gtcggcgatg
     2941 tcgtcgggcc ggaacacgat cgagatatag aagtacgcga agaacacgat ggagatcatg
     3001 tacagcagct cgtaccatgg ctcgccggcg cgcagctgat cgaaggtctt cgacagccag
     3061 gagctgttct tcacgaaagc cgcctgggca aacagcatcg gcgcggacaa aacggacgct
     3121 gcaaagataa tcggcatgac gccgccggag ttgaccttca ggggcagatg tgtggactgg
     3181 ccgcccatga ggcggcgtcc cacaattctt ttggcgtact ggacaggaat gcggcgctcg
     3241 ctgcgctcca cccagacgat gaaggccacg atggcgatca tcgccaccac gagcaggatg
     3301 agcaacggca cggtaaagac accgaaccgc tccgatctgg ccgtgctcac caggtcggcg
     3361 atgccgcggg gcaagcccac gacgatgccg gcgaagatca gaaggctcat gccgtttccg
     3421 atgccgcgct cggtgatctg ttcgcccagc cacataatga ac
//
LOCUS       DASZYN010000565         1714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_347835, whole genome shotgun sequence.
ACCESSION   DASZYN010000565 DASZYN010000000
VERSION     DASZYN010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347835"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..1643)
                     /locus_tag="VHE33_14675"
     CDS             complement(294..1643)
                     /locus_tag="VHE33_14675"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT98745.1"
                     /translation="MRSESKTPPATQGPESTSASARDFSHAWRALRHRNFRLFFGGQT
                     ISLVGTWMTRIATSWLVYRLTGSALLLGTVSFAGQIPSFVLAPFAVVIVDRYDRRQVL
                     VWTQALAMVQSLLLAWLTLTHRINITEILVLSAFQGLINAFDMPGRQSFMVQMVEDRN
                     DLSNAIAINSSMVNVARLVGPSLAGLVIAVSSEGWCFLVDGISYIAVILSLLMMHVPA
                     TGARRAAASMVEQMREGWTYVSSFVPVRTILILFAVLSLMGMPYMVLMPIFAASVLHG
                     GPHTLGFLMGAAGAGALVSAFSLVLRRSVRGLTKMIPTSAMVFGAGLFFFGMSRWLWL
                     SLILMLFVGFGMLQGMTVSNTIIQTLVPEDRRGRVMSYYTAAFMGMAPFGSLLAGSLA
                     HWIGAPRTVMVTGSFCIAGGVWFWSRLPAIRREMRPVYEQLGIIAPVPEVVEEQAAN"
BASE COUNT          396 a          623 c          432 g          263 t
ORIGIN      
        1 aacagcaacg cgcgacgaat gctaaggagg cgcgctgaac ggggcccgtc ccgcaatctt
       61 ctaggatgcg ccgcaaaatc ccggcgtcct atcctgccag agccgcagac tctccaacca
      121 aagttgtact taaaaaacag cccttgtccc gaagactcca gccggcaagt tccctattct
      181 taatttcgaa atcgtaattg ccttctgtga atccctggcg ctctctgcga ccagggattt
      241 ttttgccccg catcgaaccc gcaccctgcg ccctgtgccc cgcgccctga aaatcagttg
      301 gccgcctgct cctccaccac ctcgggaacc ggagcaatga tccccagttg ctcatacacc
      361 ggccgcatct cgcgccgaat cgccggcagc cgcgaccaga accacacccc acccgcaatg
      421 cagaacgatc ccgtcaccat caccgtgcgc ggcgcgccga tccagtgcgc cagcgacccc
      481 gccagcaggc ttccaaacgg cgccatgccc atgaacgccg ccgtgtagta gctcatcaca
      541 cgcccccgcc gatcctccgg caccagcgtc tgaatgatcg tgttgctcac tgtcatgccc
      601 tgcagcattc cgaatccgac aaacagcatg agaatcagcg aaagccacaa ccagcgcgac
      661 atcccgaaga aaaacaaccc tgccccaaac accattgccg acgtcggaat catcttcgtc
      721 agcccgcgca cagaacgccg cagcaccagc gagaaagccg acaccaacgc ccccgctcca
      781 gccgcgccca tcaaaaagcc cagcgtgtgc ggcccgccgt gcagcacgct cgccgcaaaa
      841 atcggcatca gcaccatgta cggcatcccc atcaggctca gcaccgcgaa cagaatcagg
      901 atcgtccgca ccggaacgaa actcgacaca tacgtccatc cctcgcgcat ctgctccacc
      961 atggacgcgg ccgcgcgtcg cgcccccgtc gccggcacat gcatcatcag cagagaaaga
     1021 atgaccgcga tgtagctgat gccgtccacc agaaagcacc agccctcgct cgatacggca
     1081 atcaccagcc cggccagcga cggcccaacc aggcgcgcca cgttcaccat cgaggagttg
     1141 atcgcgatcg cattcgacaa atcgttgcga tcctcgacca tttgcaccat gaacgattgc
     1201 cgtcccggca tatcgaatgc gttgatcagt ccctggaaag cgctcaggac aagaatctcg
     1261 gtgatgttga tccggtgcgt gagcgtcagc catgccagca gcagcgactg caccatggcc
     1321 agcgcctgcg tccacaccag gacctggcgg cgatcgtacc ggtccacaat cacgacggcg
     1381 aagggcgcca gcacaaagct cggaatttgc cccgcgaaac tcaccgtacc caacagcagc
     1441 gccgaccccg tcagccgata caccagccat gaggtagcga tccgcgtcat ccacgtgccg
     1501 accagcgaaa tcgtttgtcc gccgaaaaac agccggaaat tgcgatggcg cagcgcccgc
     1561 caggcatgcg aaaaatcgcg cgccgaagcg ctcgtgctct cgggcccctg cgtcgctgga
     1621 ggagttttcg attcggaccg catgctcaga acggcgttct cccgccggat gcttcagggg
     1681 aattggatgc tgcggcagaa atcctaagga tcgg
//
LOCUS       DASZYN010000566         1814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_543962, whole genome shotgun sequence.
ACCESSION   DASZYN010000566 DASZYN010000000
VERSION     DASZYN010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543962"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /gene="ureC"
                     /locus_tag="VHE33_14680"
     CDS             <1..441
                     /gene="ureC"
                     /locus_tag="VHE33_14680"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="ureases catalyze the hydrolysis of urea into
                     ammonia and carbon dioxide; in Helicobacter pylori the
                     ammonia released plays a key role in bacterial survival by
                     neutralizing acids when colonizing the gastric mucosa; the
                     holoenzyme is composed of 3 ureC (alpha) and 3 ureAB
                     (gamma/beta) subunits; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HVT98746.1"
                     /translation="GSLEKGKLADIVLWEPAYFGVKPKLVLKGGMINWSLMGDPNASI
                     TTPEPVLYRPMFGALGKAVGRTCATFLPKAAIERDVAGQLGLQHQVVPVRRCRGLQKK
                     DMVLNDRTPRIDIDPETYQVYVDGQHVTVAPAERLSLSQLYFIA"
     gene            575..1168
                     /locus_tag="VHE33_14685"
     CDS             575..1168
                     /locus_tag="VHE33_14685"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HVT98747.1"
                     /translation="MQLGQVDGSTLAALLAEHLQAGFGASDSVALACAHRAGEQARMS
                     EAIRADRRLTSVKLARETRETSCRVGRQLLNLSRRLFGQPLLDEYAQQVENGDTPGNH
                     AIVLGLSMALLGIAREQAVLSDLYAFSASFVAAGVRLNVIEYRAAQAILHQHRPLLAQ
                     VAQEACTQHVEDIASSVPWIDVMAMCHEQAEIRLFMS"
     gene            1192..>1814
                     /gene="ureG"
                     /locus_tag="VHE33_14690"
     CDS             1192..>1814
                     /gene="ureG"
                     /locus_tag="VHE33_14690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HVT98748.1"
                     /translation="MNRLIPRIGIGGPVGSGKTALIEALVPLLLEQHFKPVVITNDIF
                     TQEDAQHVRRTLAGVLDPERVLGVETGACPHTAVRDDPSMNLAAVEEMTRRFPDADLV
                     LHESGGDNLTLTYSPALADSFLFVIDVAEGDKMPRKRGPGITSSDLLVINKMDLAPYV
                     KADLAIMQRDARLVRQNRPFVFTNCLTGEGVREVADFIAQVAERAQV"
BASE COUNT          355 a          545 c          548 g          366 t
ORIGIN      
        1 ggctccctgg agaaaggcaa gctcgccgat atcgtcctct gggaaccggc ctactttggc
       61 gtcaaaccca aactggtgct caaaggtggc atgatcaact ggtccctgat gggcgatccc
      121 aacgcttcga tcaccactcc ggagccggtg ctgtaccggc ccatgttcgg cgctctgggg
      181 aaagcggtgg gcagaacgtg cgctactttt ctgccgaaag cggcgattga gcgagacgtg
      241 gccggacagt tgggactcca gcaccaggtt gtcccggtgc ggcgttgtcg tggtctgcaa
      301 aaaaaagata tggtgctcaa cgacaggacg cccagaatcg acatcgaccc ggaaacctac
      361 caggtctacg tcgatggtca gcatgtcacg gtcgcgccag ccgagcggct ttcgctctcc
      421 caactctact ttattgcctg atggcgtaca gaaaggggac atcgtgagtt caccgcacca
      481 cgctgatgcg ctggcctttc tctcgtgcct gcaactcgcc gactccttct ttcccagcgg
      541 actgtacaca ctctcgcacg gcctggagac ctgcgtgcaa ctggggcagg ttgatgggag
      601 tacgctcgct gccttgctgg ctgagcatct gcaagccggc ttcggagcaa gcgatagcgt
      661 cgcgcttgcc tgcgcgcatc gcgccggcga acaggcgcgg atgtcggagg ccatacgcgc
      721 cgacaggcgg ctcacctcgg tcaagctggc ccgcgaaacg cgagaaacct cgtgccgcgt
      781 gggacgccag ttgctgaacc tgagccggcg actctttggg cagccgctcc tcgatgagta
      841 cgctcagcag gtggaaaacg gcgacacgcc cggcaatcac gcgattgtcc tggggctgag
      901 catggccctg ctcggaatcg cgcgggaaca ggccgtgctc agcgatctct acgccttctc
      961 cgcgagcttc gtggccgccg gggtacgcct aaatgtgatt gagtatcgcg ctgcccaggc
     1021 tatcctgcat cagcacagac ctctgctggc gcaggtggcg caggaagcct gtacgcagca
     1081 tgtcgaggat atcgccagca gcgttccctg gatcgatgtc atggcgatgt gccatgagca
     1141 ggctgaaata cgcttattta tgagttaaac gtgtgaagaa agagtattcc tatgaacagg
     1201 ctcattcccc gcattggcat tggtggcccg gtcggctctg gtaaaaccgc gctcatcgaa
     1261 gcgctggttc ctctcctgct ggaacagcat ttcaagccgg tcgtgattac aaatgatatc
     1321 tttactcaag aagatgcgca gcatgtccgg cggacgctgg ccggagtgct ggacccggag
     1381 cgcgtgctcg gcgtggaaac cggtgcctgt ccgcacaccg ccgtccgtga cgatccctcg
     1441 atgaatctgg cggcggtgga ggagatgacc cggcgttttc ccgacgccga tctcgtctta
     1501 cacgaaagcg gcggcgataa cctgacgctc acctatagcc cggcgctggc cgactccttc
     1561 ctctttgtca ttgacgtagc tgaaggcgat aagatgccgc gcaaacgcgg ccctggtatc
     1621 accagctccg atctgctggt aatcaacaag atggacctgg ctccctatgt caaagccgat
     1681 cttgccatca tgcagcgaga tgcgcgcctg gttcgtcaaa accgaccctt tgtcttcacc
     1741 aattgtctga ccggcgaagg tgtgcgggaa gtcgccgatt ttatcgccca ggtggctgag
     1801 agggcgcagg tatg
//
LOCUS       DASZYN010000567         7613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_282528, whole genome shotgun sequence.
ACCESSION   DASZYN010000567 DASZYN010000000
VERSION     DASZYN010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7613
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282528"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            762..1217
                     /locus_tag="VHE33_14695"
     CDS             762..1217
                     /locus_tag="VHE33_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98749.1"
                     /translation="MVKTCRSLLSSGRPCQAPARHHSEYCRHHDPTQSHPAPRAPSQP
                     KPPKPAPADPLRWIKAAHWRNYPATLVGANEQQLQDAIDNLIEALIYRNICHRSAGRI
                     LATIANRRLQLAKQKQNAHLREYFESVARNHPEALGDKEKFFKALGQLP"
     gene            1553..2446
                     /locus_tag="VHE33_14700"
     CDS             1553..2446
                     /locus_tag="VHE33_14700"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT98750.1"
                     /translation="MRTIALRVVCLALTLVAVASAQDVIPLYPGAAPGSPEATYPEQH
                     YFSKSWNADVVTNVSKPTLTLFKPAADLKNGTAIVICPGGGYMALSIASEGTDLAKYL
                     AAKGVTAFVLEYRVAHTEGDATEQFETLYRTDQAKFQQILKTAVPLAFADGMAAVQYV
                     RAHAAQWGVSPDRVGIIGFSAGGGVTANVAFHYTPESRPAFVAPIYQGGFTPDTPPPA
                     DAPPMFIAAATDDTLGLAPASIAVYQAWVGAHKSAEIHMYAKGGHGFGMHKQNAPTDR
                     WIDAFTDWLQFEGFLTPAQKP"
     gene            complement(2462..3439)
                     /locus_tag="VHE33_14705"
     CDS             complement(2462..3439)
                     /locus_tag="VHE33_14705"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HVT98751.1"
                     /translation="MRDGRSGEVTPASDSDAPVRDRPAVPGHKLFFGPQGLRAGWAIL
                     VYATICAILIVVCGLSLSLFVHLHAGVPISPSIGLLIEMTQFLPVVGATVLMARLEHR
                     PLLSYGFQGRARGIRLVSGFVFGFVAISAVVVVLRQLGALSFDGRTMGGIAALRYAVM
                     WAAMFLCVGFCEEAMFRGYAQFTITRGMGFWWGALFFSVPFGLLHAPNPGESLVGLVG
                     AGAASLIFCLSLWYTGSLWWAVGFHASWDWGQSYFYGTADSGLVAQGHFLASHPVGQA
                     LWSGGTTGPEGSVIVIPILLVVALLMAAWWERRGEKPFRGMAWRPGRLQ"
     gene            complement(3436..4878)
                     /gene="gatA"
                     /locus_tag="VHE33_14710"
     CDS             complement(3436..4878)
                     /gene="gatA"
                     /locus_tag="VHE33_14710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HVT98752.1"
                     /translation="MSRATTAAAASIDGIRSAIARGETTARALTEIYQARIAGEDGKI
                     HAYLSVDNDHALAQAARIDSLAAKGDPLPKLAGVPFGIKDVLTLEGFASTAGSRILEG
                     YHPPYTATSVKKLADAGAIFLGKLNCDEFAMGSSNENSAYGPVHNPHALDRVPGGSSG
                     GSAAAVAAGMAVATLGTDTGGSIRQPASFCGVVGVLPTYGRVSRYGLIAFASSLDRVG
                     PFAASVRDAATVLGVIAGHDIMDATSSPLPVPDYTANLDGGVNGMKLGVPAEYFAEGL
                     DPEVRSAIEAGIEKLRAAGAEIKPISLPHTRYAIPTYYVIATAEASANLARFDGVRYG
                     FRAPQSDTLAVMYRRTRDLGFGAEVKRRILLGTYVLSHGYYDAYYRKAQQVRRLLTED
                     FLRAFREVDAILTPTAPTPAFKLGEKSDDPVAMYLADIYTVTASLAGICGVSVPCGQS
                     KAGLPIGMQILGKHFDEATIFRVGHTLETL"
     gene            complement(4875..5210)
                     /gene="gatC"
                     /locus_tag="VHE33_14715"
     CDS             complement(4875..5210)
                     /gene="gatC"
                     /locus_tag="VHE33_14715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HVT98753.1"
                     /translation="MPETERVSLDDVRRVAELANLELTADEEPRMRRDLNAILDYVAQ
                     LGELDTAQVEPMAQVSEVLASASDVPSEKQSVLRADEVRPCLDRAAVMVSAPETDSVF
                     FKVPKVIER"
     gene            5305..5478
                     /locus_tag="VHE33_14720"
     CDS             5305..5478
                     /locus_tag="VHE33_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98754.1"
                     /translation="MASEHPMNTVCQHPKVRVVAREEDVEFVECLDCGEVFDSNEFRD
                     MQIEDTQDEEETA"
     gene            complement(5549..6439)
                     /locus_tag="VHE33_14725"
     CDS             complement(5549..6439)
                     /locus_tag="VHE33_14725"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HVT98755.1"
                     /translation="MSESPNRYRGRIAPSPTGLLHAGHARTFWTAFIRSRDAGGTLVL
                     RNEDLDPQRSKADLVDAMLEDLDWLGIRWQEGPDVGGTFGPYSQSQRSEHYLRVWRQL
                     LERRSLYPCTCSRRELSGMAQAPHEDGPDRKSLRMEGDEGPMYPGTCRPTPDSPELEA
                     RRDRPAGVNWRFRVPDGEVVAFHDGHFGPQRYIAGQDFGDFPVWRRDGVPAYQLAVVA
                     DDHAMQITEVVRGADLLLSTARQILIYRALGWLEPQWYHCPLVLDERGERLAKRHDAL
                     AIRTLRGQGWTPQQVLGIAR"
     gene            complement(6469..7536)
                     /locus_tag="VHE33_14730"
     CDS             complement(6469..7536)
                     /locus_tag="VHE33_14730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT98756.1"
                     /translation="MLPYLSVIISVRNERKMLPGLIHQLLEQNYPVELYEILVVDGRS
                     TDGTGDMIRRRFSNRRVRVRVIDNPKRTVAAGRNAGIRAASGDAMVFLSGHCSIPSKN
                     LLADTAEILETTRAGCLCRPQPLLADPETRMGEAIAHARSTRLGRSSAPPDLAGFLEP
                     PGFVGVFRRSVFEEVGLFDETFDACTGLDFNERAHRAGIRAYCDPRLAVHEQPPQKLH
                     RLFVEMFRHGRGASHWMRKHPECSSLAEIAPLTVLLAVTLAIVAWVRLPHMLAALVAA
                     PIAMIPIAVLLTSMQIGSIHGFRSAWRAPAVFATIYLGQGIGLLFEYAIPSEDPRPKR
                     SAPVFSIADGVKKRSKSDRAA"
BASE COUNT         1543 a         2459 c         2323 g         1288 t
ORIGIN      
        1 ctacatcatc gaagtcggtc aatacacgca actcgccatc gactggttca acagccacag
       61 ttaatccgcc aattgggccg ctgccgcagt ttcccaatcg ccggtacgtc ggcaatgacg
      121 ccatcctgac ctgcacttca atttctcgcc ctcccatccc gacacgggtg gcgtttcctc
      181 gcaagcgagg aaacgtcagg gcgcgagggc gggcagccgg ggtcctggcg aaacaacgtt
      241 tcgccggggt ggccttgaga gtcgctgctg actacttttc gcgagccatc cgaaagccgg
      301 tgacacgtcg cctggacaat acggcgcggt gttgctctca gagcacggct tcacagctcg
      361 cggaagaatt cgattctttg aaagggccgt gagccccccg aaacccggtg acgtttcctc
      421 gcaagcgagg aaacgccagg gcacgagggt gggcggccgg ggtcctggcg aaacaacgtt
      481 tcgccggggt ggccgtgcgt gcacgaaaca accggcccac cctccaacgc gactcggctc
      541 acagcaaacc ggacccacgc gaaacaaatg tcaagccccg aacccaacgg tacgaaagtg
      601 ttcccccaca ttccagataa cttccccgcc aggacacccc aaaaagtttg gcacgatttc
      661 taactcaact tggtattcta aaaatagggg gaaatccttc cctccacctg atcccgaaag
      721 gcctcgccac ccggcgaggc ctttctcttt ggagccctcc catggttaaa acctgtcgct
      781 cgctgctctc ctccggtcgc ccctgccagg ccccggcgcg ccatcactcc gagtactgcc
      841 gccaccacga tcccacccag tctcaccccg ccccgcgcgc cccatcgcag cccaaaccac
      901 ccaaacccgc ccccgccgac ccccttcgct ggatcaaggc cgcccactgg cgcaactatc
      961 ccgccaccct cgtcggcgcc aatgagcagc agttgcagga tgccatcgac aatctcatcg
     1021 aggccctcat ctaccgcaat atctgccatc gcagtgccgg acgcatcctc gccaccatcg
     1081 ccaaccgccg cctccagctc gcgaagcaaa aacaaaacgc ccatctccgc gagtactttg
     1141 agtccgtcgc ccgcaatcac cccgaggccc tcggagataa ggagaaattc ttcaaggccc
     1201 tcggccaact accctgaatt gaattaaaaa acggcaaggc ccttgacaaa atcctgcacg
     1261 taatacgtga ccaacaactt agcagtgacc ttaacctgag aaaggaacaa gacgaatttc
     1321 ggtgcaccgc aaacctatcc ttgtctgagg tcaggtgagg gcgcggcgca cctctcggtg
     1381 ccctgtgccc tgtgccttgt gctcagcgct ctgagctctg tgctctgatt tcgactggac
     1441 aacatcagtt aacgcattga taactaccca gggggtcgac ccctcccgat gttgctgatt
     1501 cgcttcagca cccagggggg tcggaccccc ttttctgtta gactttccgg ccatgagaac
     1561 gatcgccctt cgcgttgtct gcctggcgct gaccctggtc gcagtcgcga gcgcccagga
     1621 tgtgattccg ctctaccccg gcgccgcgcc gggttcacct gaagccactt acccggagca
     1681 gcactacttc tcgaagtcct ggaacgccga cgtcgtcacc aacgtgagca agcccaccct
     1741 caccctcttc aagcccgccg cggacctgaa gaacggcacc gccatcgtga tctgtcccgg
     1801 cggcggatac atggccctgt ccatcgccag cgaaggcacg gacctggcaa agtacctcgc
     1861 ggcaaaagga gtcaccgctt tcgtgctgga gtaccgtgtg gcccacaccg agggtgacgc
     1921 caccgagcag ttcgagaccc tctaccgcac cgaccaggcg aagtttcagc agatcctgaa
     1981 gaccgccgtg cccctggcct tcgccgacgg catggccgcc gtccaatacg tccgggcgca
     2041 cgccgcgcag tggggcgtct cccccgatcg cgtcggcatc atcggcttct ctgccggagg
     2101 gggtgtcacc gccaacgtcg ccttccacta cacgcccgag agccgccccg ccttcgtcgc
     2161 gccgatctac cagggtggct tcacgccgga tacgccccct cctgccgatg cgccgccaat
     2221 gttcatcgcg gccgcaacgg acgacaccct gggcttagct ccagccagca tcgccgtcta
     2281 ccaggcctgg gtcggcgctc acaaatcggc ggagattcac atgtacgcga aaggaggaca
     2341 cggcttcggc atgcacaagc agaacgcgcc caccgatcgc tggatcgacg cgttcaccga
     2401 ctggctgcag ttcgagggct ttctgacacc ggcgcagaag ccataacgag cctgcgccga
     2461 gctattgcag gcggcccggg cgccacgcca ttccgcggaa aggcttctct ccgcgacgct
     2521 cccaccatgc cgccatcagg agagcgacga ccagcagaat tgggatgacg atcacgctgc
     2581 cctccggtcc ggtggtgccg ccgctccaca gcgcttgacc gactgggtgc gaggcaagga
     2641 agtggccttg tgcaacaagg ccgctgtcgg ctgtgccgta gaagtacgac tgaccccagt
     2701 cccaggacgc gtggaagcca accgcccacc acagcgagcc cgtgtaccaa aggctgaggc
     2761 agaagatgag actggcggcg ccggcgccca ccaggccgac cagagattca ccggggttcg
     2821 gtgcgtgcag aagcccgaag ggaacgctga agaacagcgc tccccaccag aaccccatgc
     2881 cgcgtgtgat ggtgaactgc gcgtacccgc gaaacatggc ttcttcgcag aacccgacgc
     2941 agagaaacat ggcagcccac atgactgcat agcgcagggc agcgatgccc cccatcgtgc
     3001 gaccgtcgaa ggaaagggcg ccgagttggc ggagcaccac gacgacggcg gaaatagcga
     3061 cgaagccgaa gacaaatccg gagacgaggc ggatgccgcg cgctcggccc tggaagccat
     3121 aagagagcag cggtcggtgc tccagacggg ccatcagcac cgtcgctcca acaacgggga
     3181 ggaactgagt catctcgatc agcaggccga tggatggcga gatgggaacg ccggcgtgca
     3241 aatggacaaa gagcgagagg gacaagccac agacgacgat gaggatcgcg cagatcgtgg
     3301 cgtagacgag aatggcccag cctgcccgca ggccctgcgg accaaagaag agcttgtggc
     3361 caggaacggc gggccggtcg cggacgggag cgtcagagtc agaagcgggt gtcacttcgc
     3421 ccgaacgccc gtctctcaaa gcgtctccag cgtgtggcca acgcggaaga tcgtagcctc
     3481 gtcgaagtgc ttgcccagga tttgcatgcc gatgggcagg ccagccttcg attgtccaca
     3541 cggaaccgag acgccgcaga tgccggccag gctggcggtg acggtgtaga tgtcagccag
     3601 atacatggcc accggatcgt cggacttttc gccaagctta aaggcaggcg ttggcgcggt
     3661 aggcgtgagg atcgcgtcga cctcgcggaa tgctcggagg aagtcctcgg taagcagacg
     3721 gcgaacctgc tgcgccttcc ggtagtacgc gtcgtagtag ccgtggctga gaacgtaagt
     3781 gccgagcagg atgcgtcgct tcacttcggc gccgaagccc aaatcccgcg tccgccggta
     3841 catcacagcc agcgtgtcgg actgcggcgc gcggaaaccg tagcggacgc cgtcaaaccg
     3901 agccaggttg gcactggctt cagccgtagc gatgacgtag tacgtgggga tggcgtaacg
     3961 cgtgtgcggc aatgagatcg gcttgatctc ggctccggca gcacgcagct tttcgatacc
     4021 ggcttcgatc gccgatcgca cttcggggtc cagaccctcg gcgaagtact cggcgggaac
     4081 gccgagcttc attccattca cgccgccgtc gaggttcgca gtgtaatcgg gcaccggcag
     4141 cggcgaggag gtcgcatcca tgatgtcgtg accggcgatg acgccgagca cagtcgcggc
     4201 atcgcgcacg ctcgcggcaa acgggccgac gcgatccagc gatgacgcaa aggcaatcag
     4261 gccataccgc gagacgcgcc catacgtggg cagcacgccg acgaccccac agaacgatgc
     4321 cggctgccgg atcgatccgc cagtatcggt gccgagcgtc gccacggcca tgcctgcggc
     4381 caccgctgcg gcggagccac cgctggaccc gcctggcacg cgatcgagcg cgtgcggatt
     4441 gtgcaccggc ccgtaggcgg agttttcgtt ggacgagccc atggcgaact cgtcgcagtt
     4501 gagcttgccc agaaagatgg cgcccgcatc ggcgagcttc ttcaccgacg tcgcggtgta
     4561 aggcgggtga taaccttcga ggatcctcga accggcggtc gaggcaaagc cttcgagagt
     4621 gaggacatcc ttgatgccaa agggaacgcc ggccagcttt ggcagcggat ctcctttggc
     4681 ggcgagcgaa tcgatgcgag ccgcctgggc cagtgcgtgg tcgttgtcga cgctgagata
     4741 cgcgtggatc ttcccatcct cgcccgcgat gcgcgcctgg tagatttccg tcagtgccct
     4801 cgcggtcgtc tcacccctgg caatggcgga gcggatgccg tcaatggaag cggcggcggc
     4861 cgtggtcgcc ctgctcatcg ctcgatcacc ttcggcactt tgaagaaaac ggagtccgtc
     4921 tccggggcgg aaaccataac ggctgcgcgg tcgagacagg gccggacttc atcggcgcgc
     4981 agaacggatt gtttctcgga cggcacatcc gaagcggagg cgaggacctc ggagacctgg
     5041 gccatcggct cgacctgcgc ggtatccagt tcacccagct gcgcgacgta atcgaggatg
     5101 gcgttgaggt cgcgacgcat gcgcggctcc tcgtcggctg tcagctcgag attagccagc
     5161 tcggcgacgc ggcgcacatc atccagggaa actcgttccg tttcgggcat ggttccagtt
     5221 taaccggcac gccacaggac ggccgaacaa ggctgttgac agacgcgcgt agccaggtac
     5281 aatctgcccc ataccgaatg ccagatggct tctgaacacc ccatgaatac ggtttgccag
     5341 catcctaagg tccgcgttgt ggcgcgcgag gaagatgtcg agttcgtcga gtgcctggac
     5401 tgcggagagg tcttcgactc caacgagttt cgcgacatgc agattgagga cacgcaggac
     5461 gaggaagaga cggcctgaat tctctctgac gtccttcgct ccgcgcagga tttccctgcg
     5521 ggcccggacg tccgcaaatc gtctcaatct acctggcaat gcccagcact tgctgcggcg
     5581 tccaaccctg tccgcgcagg gtgcggatag ccagcgcatc gtgacgcttg gcaaggcgtt
     5641 cgccgcgttc gtcgaggacg agggggcagt ggtaccactg tggctcgagc cagccgagcg
     5701 cccgataaat caggatctgg cgagcggtgg agaggagcag gtcggcaccc cggacaactt
     5761 ccgtgatctg catcgcgtgg tcgtcggcga ctaccgccag ctgatatgcg ggaacaccgt
     5821 cacggcgcca gacaggaaag tcgccaaagt cctggcctgc gatgtatcgc tgagggccga
     5881 agtggccgtc gtggaaagct actacctctc catcgggaac tcgaaagcgc cagttgacgc
     5941 ccgcaggacg atcgcgtcgc gcctctaact ccggagaatc gggcgttggg cggcaggtgc
     6001 cgggatacat gggcccttcg tcgccttcca tccgcaacga tttgcggtca ggtccgtctt
     6061 catggggtgc ctgtgccatg ccgctgagct cgcggcgaga gcaagtgcag ggatacaggg
     6121 aacgtcgctc cagaagctgc cgccacaccc tcaggtaatg ttcgctccgc tgactttgag
     6181 aataagggcc gaatgtccca ccaacatccg gcccttcctg ccaacggatg ccgagccagt
     6241 caaggtcctc gagcatcgca tcgacgagat ccgccttcga acgttgcgga tcgagatcct
     6301 cgttgcgcag aaccagcgtt cctccggcat cccgcgaccg tataaatgcg gtccagaaag
     6361 tgcgtgcgtg tccagcgtgc agcaatccgg tcggcgaggg cgcgatgcgt ccgcgatacc
     6421 ggttgggaga ttcgctcacg ccgacagttt agcggcccga gccctgcgtc aggcggcgcg
     6481 atcggatttg ctgcgcttct tcaccccatc ggcaatgctg aacaccggag cggatctctt
     6541 cggtcgcgga tcttccgacg gaatcgcgta ttcgaacagc agaccaattc cctgccccag
     6601 gtaaatcgtg gcgaagaccg ccggtgctct ccatgccgag cggaatccat gaatcgagcc
     6661 gatctgcatg gaggtcagca ggaccgcgat cgggatcatt gcgatgggcg cagctacaag
     6721 ggcggccagc atgtgcggca gccgaaccca ggcgacgatg gcaagcgtta cagccagcag
     6781 aacggtgagc ggcgcgattt cggcaagcga ggagcattcc ggatgcttcc gcatccagtg
     6841 gctggcgccg cgtccgtggc ggaacatttc aacgaacagc ctgtggagtt tctggggtgg
     6901 ctgttcatgg acggccaggc gcgggtcgca ataggcgcga atgccggcgc ggtgagcacg
     6961 ttcattgaaa tccagaccag tgcaagcgtc gaaagtttca tcgaagaggc ccacttcttc
     7021 gaacacgctg cgccggaaga cgccgacaaa gcccgggggt tcgaggaagc cagccagatc
     7081 gggcggagca gagctgcggc cgagccgggt ggagcgcgcg tgcgcaatgg cctcgcccat
     7141 gcgcgtctcg ggatcggcaa gcagaggctg gggacggcac aggcatccgg ctctggtggt
     7201 ctccagaatt tccgctgtgt ccgccagcag gtttttggat ggaatcgagc aatgcccaga
     7261 gagaaacacc atagcgtcgc cggatgcggc gcgaataccg gcgttgcgtc cagccgcgac
     7321 ggtgcgcttc gggttgtcga tgacccgcac gcgtacgcga cgattgctga agcggcggcg
     7381 aatcatatcc cccgtcccgt cggtggaccg tccgtcgaca acaagaatct cgtacagctc
     7441 gactggatag ttttgctcca gcagctgatg gatgagccca ggcagcattt tccgttcgtt
     7501 gcgaacggaa ataatcacgg agagataagg cagcattcga cgtcctcttt gtacgtctta
     7561 ctgcttgttc gatgctcgtt tataccattg aaaatcaaca tctaacgcct gtt
//
LOCUS       DASZYN010000568         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_32788, whole genome shotgun sequence.
ACCESSION   DASZYN010000568 DASZYN010000000
VERSION     DASZYN010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32788"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VHE33_14735"
     CDS             complement(<1..761)
                     /locus_tag="VHE33_14735"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HVT98757.1"
                     /translation="MAYVLALDQGTTSSRAILFDKQGNIAGVAQHEFPQIFPQPGWVE
                     HNPIDILTSQLSAAVDVLGKVSATPRDVVALGITNQRETAIVWDRKTGKPVANAIVWQ
                     DRRTAPMCARLREEGVEETIRAKTGLLLDPYFSATKIAWMLENVDGLRARAERGELAF
                     GTVDSWLIWNLTEGKRHVTDRTNASRTLLYNIVEDRWDEELLRIFGVPASMMPEVVWS
                     SEEVGRVTAALGPAAVPIAGIAGDQQSALFGQLCT"
     gene            873..1406
                     /locus_tag="VHE33_14740"
     CDS             873..1406
                     /locus_tag="VHE33_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98758.1"
                     /translation="MPYQIQDFEREYAGKSNDDLLRLAWAPQDLVDDAKIALQTEMGR
                     RGLKSADIDAARFEKEREEEREERKAANRMLQTQWRGIGFQRFCKWDRTYDPDSQTEE
                     FTTTRFFILFQLPLIPLATFRVRRRKGIFQKLHILETLPLNWAQVLWVWMLTLLAILA
                     LFLLARYGLPLLDRHKP"
     gene            complement(1410..>1606)
                     /locus_tag="VHE33_14745"
     CDS             complement(1410..>1606)
                     /locus_tag="VHE33_14745"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HVT98759.1"
                     /translation="VGVTKDLTSKVQAGKVVGEIAKKVGGSGGGRPDMAEAGGKDASA
                     LDGALKGAADVVRQLLGASS"
BASE COUNT          326 a          577 c          395 g          308 t
ORIGIN      
        1 ctggtgcaca gttgcccaaa cagcgccgac tgctgatccc ccgcaatccc cgcgatcggc
       61 acagccgccg gacccagcgc cgccgtcacc cttcccactt cttccgacga ccacaccacc
      121 tccggcatca tgctcgccgg cacgccgaag atccgcagca gttcctcgtc ccagcggtct
      181 tccacaatgt tgtagagcag cgtccgcgac gcattcgtcc ggtcggtcac atgccgcttg
      241 ccttcggtca gattccatat cagccagctg tccacggtcc caaacgccag ctctccgcgc
      301 tccgctcgcg ctcgcaggcc gtcaacgttc tcgagcatcc acgcaatttt ggttgccgaa
      361 aaatacggat cgagcagcag ccctgtcttc gcccgaatgg tctcttcgac gccttcttcg
      421 cggagccgcg cgcacatcgg cgcagtgcga cgatcctgcc agacaatcgc gttcgcgacc
      481 ggcttgcctg tcttccgatc ccacacaatc gccgtctcgc gctgattcgt gatccccagt
      541 gcgaccacat cgcgcggggt cgcgctcacc ttgcccagca cgtccaccgc cgcgctcagc
      601 tgcgacgtga gaatgtcgat gggattgtgc tccacccacc ccggctgcgg aaaaatctgc
      661 gggaactcat gctgcgccac gcccgcaatg ttcccctgct tgtcaaaaag aatcgcccgt
      721 gaactcgtcg tgccctgatc cagcgctaaa acataagcca tcgcgaatca cccgcaacag
      781 gataacgggt gtcgcgaaaa cctgaatttg aaggtcgaaa aagaaaagtc cgtaccgatc
      841 taattcgcgg gattatcctg cgaatcatca ccgtgcctta tcaaatccag gacttcgagc
      901 gcgaatacgc cggcaaatcc aatgacgacc tgctccgcct cgcctgggct ccgcaggatc
      961 tggtcgacga tgcaaaaatc gcgctgcaaa cggagatggg ccgccgcgga ctcaagagcg
     1021 ccgatattga tgcagctcgt tttgagaagg aaagagagga ggaacgagag gagcgaaagg
     1081 ccgccaaccg gatgctccag acccagtggc gtggaatcgg tttccagcga ttctgcaaat
     1141 gggatcgaac ttacgatccc gactcacaaa ccgaagagtt caccacgact cggttcttca
     1201 ttctcttcca gctgccgctc attcccctgg ccaccttccg cgttcgccgc agaaaaggca
     1261 tcttccagaa gctccacatc cttgaaacgc taccgctcaa ctgggctcag gtcctctggg
     1321 tgtggatgct gaccttactc gcaatcctgg ccctttttct ccttgcacgc tacggcctgc
     1381 cgctgctcga ccggcacaaa ccctaacagc taagagctag cacctaaaag ctgcctcacc
     1441 acatccgccg cgcccttcag cgccccatcc agcgccgacg catccttccc gccggcctcc
     1501 gccatgtccg gacggccgcc gcccgatccg cccaccttct tcgcgatctc gccgacgact
     1561 tttcccgcct gcactttgct ggtcaggtct ttcgtgacgc caacga
//
LOCUS       DASZYN010000569         9382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_932185, whole genome shotgun sequence.
ACCESSION   DASZYN010000569 DASZYN010000000
VERSION     DASZYN010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 9382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9382
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932185"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..437
                     /locus_tag="VHE33_14750"
     CDS             48..437
                     /locus_tag="VHE33_14750"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HVT98760.1"
                     /translation="MIVDSSAIVAIIRQEPEAAEFARTIAQNGCRLSAVNYVEAAVVI
                     DSVRDAVVSRKFDELIRESEISIEPVTESQARIAREAYRDFGKGSGHPAQLNFADCFA
                     YALAKDFAEPLLFKGRDFSQTDLTPAL"
     gene            complement(450..1523)
                     /locus_tag="VHE33_14755"
     CDS             complement(450..1523)
                     /locus_tag="VHE33_14755"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HVT98761.1"
                     /translation="MTEPIRVGVVGFGLAGRIFHTAVIQATPGLELAAVVQRSGDDAK
                     KEYPEAHLARSVEEMLADVGIQLVVIATPSYSHYELAKQCLHEQRHVVIDKPFTLTSE
                     EAAKLITMARHRNLTVTAYQNRRWDGDFKTVRQVIASGELGRLVSFESHFDRFRPAPR
                     SDVWRESGGPGGGTLFDLGPHLIDQALTLFGTPASLTANVRVEREGAAVDDAWDVFLE
                     YEQPRRMTVLLRATSSACASGPRYILHGTKGSFTKWRLDPQEEQLKAGMRPDSPGFGE
                     EPESAWGELKLCAEPSPVIRRVKTERGDYRGFYENVRDVLWGKAELEVTPEEAWRTTR
                     LIELARESSAQGRRIRVDFSAQL"
     gene            complement(1542..3233)
                     /gene="recN"
                     /locus_tag="VHE33_14760"
     CDS             complement(1542..3233)
                     /gene="recN"
                     /locus_tag="VHE33_14760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HVT98762.1"
                     /translation="MLLELRAENYAVIDHAIAGFGPGLNLLTGETGAGKSILVDALAL
                     LMGQKASSGIVRHGADRAVLSCVFEATPGAEQVLESNGIDIDGDEVILRREILADGKG
                     RVYVNNQPATVAVLRALAPELALVHAQSETLSGFDPGQQRLLLDRFGGVSTDATAEAF
                     AGWRAIQDRLAELEKDEQDRLRLADLWSFQAREIQSAAPEAGEDESLETERRVLMNAE
                     KLHAAATAVTDALYEGDASAESTLQAAQKHLEELARYDASFSESVQQIASARAVVEDV
                     SATVRDYAERIDASPERLAAIEDRLALLDRLKRKYGGTLAEVIAFGEEAARKLAEIEN
                     RDEILKTLRADLAKAADAYRTAARALTAERTAAAKKLEKLAESQINDLAMKARFSIAV
                     AVDEDPTAWTAHGWDTVECRIATNPGEPMQPLDQIASGGEMSRVLLALKVSVEEGVSR
                     GRTKRTQVPRTLVFDEIDIGIGGRAAEAVGQKLKALSRVQQVLCITHLPQIAAFADQH
                     LLIEKQEKQRRTQTSIRLLTAQERAGEIARMISGATVTETSLRHAEQMLKSSAAV"
     gene            complement(3274..3774)
                     /locus_tag="VHE33_14765"
     CDS             complement(3274..3774)
                     /locus_tag="VHE33_14765"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HVT98763.1"
                     /translation="MSQLQARLAGVFYLLTIVVGVFAAFARSRPLSDAGNLMGTACYV
                     VVTILLYLILKPVNPALSLIAAAISLAGCTVGALEALHRSPFHFRGILIFGFYCLLLG
                     YLIFRSRFFPRWLGFLVALAGVGWLTFLWPPLVRHLGLLPMLIGLIGEGSLTVYLLVA
                     PTKSKA"
     gene            3911..5203
                     /locus_tag="VHE33_14770"
     CDS             3911..5203
                     /locus_tag="VHE33_14770"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HVT98764.1"
                     /translation="MRALSALCLLLTCCSAWPQSPTPQRPLLIGYITRISSGSDFDVN
                     GVRILCGGETRIQSPARGDASFIDMGCFRTPPVVGQRVEVYGHRKKQPNAVVAERIDI
                     KRIHHDDISGWALIDAIADPDPSAPRVVRIRADGYWMTITPRTRVAFNDPLHSISDVM
                     TNVWVRYSAKPGPDGTYAAQKAKFFQNSITENQDAKRAKTDYDPATVPANAKQNALAA
                     TIEGPNPKQMAPWPDEAMQHRVSAIGHKLVPSWENKLADSDPTKINFRFQVTSGHGWP
                     LPIALPSGVVLVPHESVERMQNDSQLAAILADAMACVLEKQYFRMSGANNGITAGTGT
                     EVATILFSPAWGAAVTSVAAKIHRDQRQSARVSLDLMHDAGYDVDEAPKAWWLLDSRK
                     PQPLEEIAMPDRAAYLYRVLGEVWGGTPGMAQSGTGDE"
     gene            complement(5210..6655)
                     /locus_tag="VHE33_14775"
     CDS             complement(5210..6655)
                     /locus_tag="VHE33_14775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT98765.1"
                     /translation="MEGTTAESQLRTRTVTTQELPQLIRRIPILSKLREEDLGCLGEV
                     QLVEVPAGTMLVQQGGPVPGFCMILEGEIRAFRVELNGTETPVMSYQEGEAFGEAPLL
                     MGSKKAGVQCVATRDLQLLVVSEENFWRLMATCPTVRQNVLATAAQRVQAFQASTLHK
                     EKLVSLGTLAAGLMHELNNPGSAAKRSAALLRENLTRLQDLSMRFCRSPLTVEQATCL
                     LDMQREVLGPDKSQAMSSLEEADAEEELGTWLEEIGVNNAWKLAPTLVAAGWRRNDIA
                     CAQGAFPGETLQVALNWLEALISAMQQLTTIEESIGRVTDLVVAVKKYAYEDKNGEHV
                     IDVHENIVSTLTILGHKFRHKSLTIEKSFDPNLPKIKTRGTGLSQVWTNLLDNAIDAA
                     PEGSKIRIRTWSENGNLCVGIADQGAGVDPEIREQIFQPFYTTKPPGVGTGLGLDIAK
                     RIVTAQYNGSIGFTSEPGKTEFVVRLPAPKA"
     gene            complement(6694..8346)
                     /locus_tag="VHE33_14780"
     CDS             complement(6694..8346)
                     /locus_tag="VHE33_14780"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVT98766.1"
                     /translation="MARPIILAVDDDASVLEAVVQDLRRKYGQSYRILRAGSGQAALD
                     TCAQLKDRGDVVALIVSDQRMPGMAGVELLERVQEQYPQARRVLLTAYADTEAAIRAI
                     NTARIHYYLTKPWDPPEERLYPVLDDLLEDWNEGFKPPFEGIRVVGPRYGLLDYRVRN
                     FLSRNQVPYVWLDPERDDQAVEMLQRFNLGDSKLPVVLFQDGTSLVQPQEDELAQKVG
                     LRTQAKQDFYDLVVAGAGMAGLAAAVYGASEGLKTLVLEAEAPGGQAGSSSRIENYLG
                     FPTGISGERLARAAYDQARRLGAEFITQRATGITADGQYRHVRLANGTELSCRVSLVA
                     VGVCYRRIDAPGIDRLTGSGVYYGASPAEAQSCKDEVVFVVGGANSAGQAAMHFSRVA
                     KQVTMLVRGDGLVSSMSKYLIDQIAGTSNIKVETRAEVVEAMGNSHLESLKIRTPQGE
                     EVRPASSLFLFIGAAPQTDWLPESIMRDPNGFVLAGRDLRVEGQLPKSWKEDREPYLL
                     ETSMPGVFVAGDVRHGSVKRAATAVGEGSMAVQLMHQYLAQF"
     gene            8802..>9382
                     /locus_tag="VHE33_14785"
     CDS             8802..>9382
                     /locus_tag="VHE33_14785"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT98767.1"
                     /translation="MNKLILADNQAIYRAGTAKILAMEDDFRIIAQCAEADRLFPAID
                     TFRGAILVFASALKLDPQTLVPRVAAAGSRSIVIAENGESVQAWFNSGVQGAAHRNVT
                     GPALIDCVRHVGAGDRNVRAAGVDLPVTEDDSVGSRVRDRLTPKELKIVALIVQGCKN
                     KEIAVRLNTTEQVIKNYLRSVYDKTGVSDRLELA"
BASE COUNT         1781 a         3091 c         2715 g         1795 t
ORIGIN      
        1 aaggtgaacc ccgacgacct gctgttcgac gataaagggc ttccgaaatg atcgtcgact
       61 catcggcgat cgtggccatc attcgccagg agccggaagc cgccgagttc gctcggacga
      121 tcgcccagaa tggctgtcgc ctgtcggcag tcaattatgt ggaggcggcg gtagtcatcg
      181 acagtgtccg cgacgcggtc gtcagccgga aattcgatga gctgattcgc gagagtgaaa
      241 tatccatcga gccggtcacc gaaagtcagg cgcgcatcgc tcgcgaagcg tatcgcgact
      301 tcggcaaagg cagtggccat ccggcgcagc tcaacttcgc cgattgcttc gcttatgcgc
      361 tcgccaaaga ttttgccgag ccgctgctct tcaaaggccg cgacttctca cagacagacc
      421 tgactcccgc cctttgagcg gctcagccat cacagctgcg cgctgaagtc gacacgaatc
      481 cgtctgccct gagcactgct ctcccgcgcc agctcaatta gccgcgtcgt tcgccatgcc
      541 tcctccggcg tcacttccag ctccgccttc ccccaaagca catcgcgaac gttctcgtag
      601 aatccgcgat agtctccacg ctcggtcttc acgcgacgta tcaccggact cggttcagcg
      661 cacaacttca gctcgcccca cgccgattca ggttcctctc caaaaccggg agagtcgggg
      721 cgcataccgg ccttcagctg ctcttcctgg ggatcgagcc tccacttggt gaaactgccc
      781 ttcgtgccgt ggagaatgta gcgcggaccc gacgcacagg ccgacgatgt cgcccgcagc
      841 aggaccgtca tccggcgcgg ctgctcatac tccaggaaaa catcccacgc gtcgtcgacc
      901 gccgcgccct cgcgctccac gcgcacgttt gcagtcaggc tggccggtgt gccgaaaagt
      961 gtcagcgcct ggtcgatgag gtgggggccc aggtcgaaca gggttccgcc gccgggaccg
     1021 ccgctttcgc gccacacatc gctgcgtggc gccggacgaa agcggtcgaa atgcgattcg
     1081 aagctcacca ggcggcccag ctcaccgctc gcgatcacct gtcgcaccgt tttgaagtcg
     1141 ccatcccagc gacggttctg atacgccgtc acggtgagat tccggtggcg cgccatcgtg
     1201 attaatttgg ccgcctcttc cgaggtcagc gtgaagggct tgtcgatcac cacatgccgc
     1261 tgctcgtgga ggcactgctt cgccagttcg taatgggagt aactgggggt cgcgatcacc
     1321 accagctgaa tgcccacgtc ggcgagcatc tcctcgacgc tgcgcgccag atgcgcctcc
     1381 ggatactcct tcttcgcgtc atcgccgctg cgctgcacga cggcggcgag ttccagtcct
     1441 ggcgttgcct gaatcacggc ggtgtggaag atgcgtcctg ccagcccgaa gccaacaacg
     1501 ccgactctga tcggttctgt catagggagc attatctacg ctcagacggc tgcgctggac
     1561 ttcagcatct gctcggcgtg ccgcagagag gtttccgtga ccgtcgctcc ggagatcatg
     1621 cgcgcgatct ctcctgcgcg ctcctgcgcg gtcagcaggc gaatcgaagt ttgcgtccgt
     1681 cgctgcttct cctgcttctc gatcagcagg tgctgatcgg cgaacgcggc gatctgcggc
     1741 aggtgggtga tgcacaacac ctgctggacg cgggagagcg ccttgagctt ctggccgacg
     1801 gcttccgcag ctcgtccgcc gatgccgatg tcgatttcgt caaaaacgag cgttcgcggt
     1861 acctgcgtcc tcttcgtgcg cccccgcgat acaccctctt ccacgctcac cttcagagcc
     1921 agcagcaccc gcgacatctc ccctccggaa gcaatctggt cgagcggctg catcggctcg
     1981 cccgggttcg tcgcgatgcg gcattcgacc gtatcccagc catgcgccgt ccacgcggtt
     2041 ggatcctcgt ccaccgccac cgcgatcgag aaacgcgcct tcatcgcgag atcgttgatc
     2101 tgcgactcgg ccagcttctc cagtttcttc gccgccgcgg tgcgttcggc ggtcagcgcg
     2161 cgtgccgctg ttcgatacgc atcggcagcc ttggccagat cggcgcgcag cgttttcagg
     2221 atttcgtcgc ggttctcgat ctcggcaagc ttacgggcag cttcctcgcc gaatgcgatg
     2281 acttcagcca gcgtccctcc gtatttgcgc ttcagccggt cgagcagcgc gagccggtct
     2341 tcaatcgcgg caagacgttc cggacttgca tcgatgcgtt cggcataatc gcgtaccgtc
     2401 gcgctcacgt cttcaaccac ggcgcgcgcc gaggcaatct gctggaccga ttcgctgaag
     2461 gacgcgtcat aacgcgcgag ttcttccaga tgcttctgcg cggcctgcag ggtcgattcc
     2521 gccgacgcat cgccttcgta gagtgcatcg gtgacggcgg tcgccgcggc gtgcagcttt
     2581 tcggcgttca tcagaacacg gcgttctgtc tcgagagatt cgtcctcgcc cgcctcaggc
     2641 gccgcggatt gaatctcgcg cgcctggaaa ctccacaaat cggcgaggcg caggcgatcc
     2701 tgctcatctt tctcgagctc ggcgaggcga tcctgaatcg cacgccagcc ggcaaaggct
     2761 tcggccgtgg cgtcggtgga aacgccgccg aaccggtcca gaagtagccg ctgctgcccg
     2821 ggatcgaagc cggacagcgt ttccgactgc gcatgaacca gtgccagttc gggcgcaagg
     2881 gcacgcagaa ccgcaaccgt cgcaggctga ttgttgacgt aaacgcggcc tttgccgtcg
     2941 gccaaaattt cgcgccgcag gatgacctcg tcgccatcga tgtcgatgcc gttcgactca
     3001 aggacctgct ccgcgccggg agtcgcttcg aagacgcagg agagaaccgc gcggtccgcg
     3061 ccgtggcgga cgatcccaga tgaggccttc tgccccatga gcagcgcgag agcgtccacc
     3121 aggatggatt tgccggcgcc ggtttcaccg gtcagcaggt tcagcccggg tccaaacccg
     3181 gcgatggcgt gatcgatgac cgcgtagttt tcggctcgaa gttcgagcag catggaattc
     3241 agggtacagg gtacagggcg cagggtacag agttcaggct ttggatttgg tcggggccac
     3301 gagcagataa acggtcagcg aaccttcccc gatcagcccg atcagcatcg gaagcagtcc
     3361 gaggtgccgg accagaggcg gccacagaaa cgtcagccac ccgacacccg ccagagcgac
     3421 caaaaaacca agccagcgtg gaaagaatcg cgaccgaaaa atgagatatc caagcagcag
     3481 gcagtagaag ccaaagatca gaatgcccct gaagtgaaag ggtgagcgat gcagcgcctc
     3541 cagcgcgccc accgtacaac ccgccaggct gatcgccgcc gcaatcagcg agagtgccgg
     3601 gttcaccggc ttcaggatca gatacagaag aatcgtgacg acgacgtagc aagccgtgcc
     3661 catgagattg ccggcatcgc tcagcggcct cgagcgagca aaagcagcga acacacccac
     3721 gacgatggtc agcagataaa agacaccagc gagcctggcc tgcaattggg acacacgcgc
     3781 cacccttccg gggccagagg atcgagggag agcctgccgt ttccatacgg acagcacgcg
     3841 ctgatagttc ccaaaggcgt gccctgaatt cgccatcgcc gcatctgatt gcagcagcta
     3901 aactgcggcc atgcgggccc tctctgctct ttgtctgctc ctcacatgtt gttctgcctg
     3961 gcctcagtcc cccacaccac aacggcctct gctcattggc tacatcacgc gcatctcctc
     4021 cggctcggat ttcgacgtca acggcgtacg cattctttgt ggaggcgaaa cacggattca
     4081 atcgccggcg cggggcgatg cctcatttat tgacatggga tgttttcgca ctcccccggt
     4141 tgtggggcag cgggtagaag tctacggaca tcggaagaaa caaccgaatg ccgtggtcgc
     4201 tgaacggatc gacataaaac ggattcatca tgacgacatt tccggctggg cgttgatcga
     4261 cgccattgcc gatccagacc cctccgcgcc cagggtagtc cgcattcgcg cagatggtta
     4321 ctggatgacg atcacgccca ggacccgcgt tgctttcaat gatccgctgc attcgatctc
     4381 agacgtgatg acaaatgtct gggtgcgata cagcgcgaag cccggccccg acgggactta
     4441 tgcagcacag aaagcaaaat tctttcagaa ctcgataacc gaaaaccagg acgcgaaacg
     4501 cgccaaaacg gattacgatc cggcgacagt cccggcaaat gcgaagcaga atgcgctggc
     4561 cgctaccatc gaaggcccca atccaaaaca aatggctccc tggccggacg aagcgatgca
     4621 acaccgcgtc tccgcaatcg gacacaaact ggtgccttcc tgggaaaata agcttgcgga
     4681 cagcgatcct acaaaaatca attttcgctt tcaagtcaca agcggtcacg gttggccact
     4741 gccaatcgca ctgcccagcg gcgtcgtcct ggttcctcac gaatcggtcg aacgtatgca
     4801 aaacgactcg caacttgccg ctatccttgc cgatgccatg gcctgtgtgc tggaaaagca
     4861 gtacttcaga atgtcaggag cgaacaacgg tatcaccgcc ggcactggca ccgaagtcgc
     4921 gacgattctg ttctctcccg cttggggcgc agccgttacc agtgtcgcgg ccaagattca
     4981 cagggatcag cgtcaaagtg cccgggtgag cctggatctc atgcacgatg ccggttacga
     5041 cgtggacgaa gccccaaagg catggtggct gctcgactcc agaaaaccac agccgctgga
     5101 agaaatcgca atgccggatc gcgcggctta cttgtatcgg gtgctgggtg aagtgtgggg
     5161 cggaaccccc ggcatggcgc aaagtggcac tggagacgaa tgagacgccc tacgccttcg
     5221 gcgcaggcag tcgaacgaca aactccgtct tccccggctc cgacgtaaat ccgatgctgc
     5281 cgttgtactg ggcggtcaca atccgtttcg cgatgtcgag tcccagcccg gttcccacac
     5341 ccggcggctt cgtcgtgtag aagggctgaa aaatctgctc gcgaatctca gggtcgaccc
     5401 cggccccctg gtcggctatg cccacgcaga ggttgccgtt ttcactccag gtacgaatgc
     5461 gaatcttcga tccttccgga gccgcgtcga tcgcgttatc gagcaggttg gtccatacct
     5521 ggctcagtcc cgtaccgcgc gtcttgatct tcggcagatt cggatcgaag ctcttttcga
     5581 tcgtcagtga cttgtgccgg aatttgtggc cgaggatggt cagcgtgctg acgatgtttt
     5641 cgtggacgtc gataacatgc tcgccgttct tgtcctcgta ggcgtacttc ttcacggcga
     5701 ccaccagatc ggtgacgcgg ccaatgctct cctcgatcgt cgtgagctgc tgcatggcag
     5761 agatcagcgc ttccagccag ttgagcgcca cctgcagggt ttcaccgggg aaagcgccct
     5821 gggcgcacgc gatatcgttg cgtcgccagc cggcagccac aagggtggga gccagcttcc
     5881 aggcgttgtt gacgccgatc tcttccagcc acgtacccag ttcctcttct gcatcggcct
     5941 cttcaaggct gctcatggcc tgggatttgt ccgggccgag cacttcgcgc tgcatgtcga
     6001 gcaggcacgt tgcctgttcg accgtcagcg gcgaacggca gaagcgcatg ctcaggtcct
     6061 gcaggcgggt cagattctcg cgcagcaacg ccgcggagcg cttggctgcc gagccggggt
     6121 tgttcagctc gtgcatcagg ccggccgcca gcgttccgag agaaaccagc ttttccttgt
     6181 gcagcgtgct tgcctggaaa gcctgaacgc gctgcgccgc ggtggcgagc acgttttgcc
     6241 gcacggtggg gcaggtggcc atcaaccgcc agaagttctc ttcactcacc accagcagtt
     6301 gcaaatcgcg ggtggcgacg cactgcacgc cggctttttt cgagcccatc agcagcggcg
     6361 cctcgccaaa ggcctcgccc tcctggtagc tcatcaccgg tgtttcggtc ccattcagct
     6421 ccacacgaaa ggcgcgaatc tcgccctcga ggatcatgca gaacccgggc accggccctc
     6481 cctgctgcac cagcatcgtt cccgcgggaa cttccaccag ttgcacttcg cccagacagc
     6541 ccaggtcttc ttccctgagc ttgctgagga tgggaatacg gcgaatgagc tggggcagct
     6601 cctgcgttgt cactgtcctt gttctcagct gcgattccgc cgtcgtgcct tccatctgct
     6661 cctttttcct ttagaactgc gccaaaataa aaatcagaat tgtgccagat actgatgcat
     6721 cagctgcacc gccatcgaac cttcaccgac cgcggtggcc gcgcgcttga ccgaaccatg
     6781 ccggacgtcg ccggcgacga acacgcccgg catcgatgtt tccagcagat aaggctcgcg
     6841 gtcctctttc caggatttcg gcagctgccc ttccacgcgc agatcgcgcc ctgcaagtac
     6901 aaacccgttc ggatcgcgca tgatgctctc cggcagccag tccgtctgcg gcgccgcccc
     6961 aatgaaaagg aacagcgacg aggcggggcg aacttcctcc ccttgcgggg ttcggatctt
     7021 caggctctcc aaatggctgt tccccatcgc ttcgaccacc tcggcccgcg tttccacctt
     7081 aatattcgat gtgccggcga tctgatcgat gagatatttc gacatactgc tcaccagccc
     7141 atccccgcgc accagcatcg tcacctgttt ggccacccgg gagaagtgca tggccgcctg
     7201 accggccgaa ttagcccctc ccacgacaaa caccacttcg tccttacacg actgcgcttc
     7261 cgccggggat gcgccgtaat atacccccga gccggtcagg cgatcgatgc cgggggcgtc
     7321 gatgcgccga taacagaccc ccaccgccac caggctgacc cgacaagaga gctctgtgcc
     7381 gttggccagg cgcacatgcc ggtactgtcc atcagccgtg attccggttg cgcgctgggt
     7441 gatgaattcc gcccccaaac gccgagcctg gtcgtaggcc gctcgcgcca gacgttcccc
     7501 gctgatgcca gtaggaaagc ccaggtagtt ttcgatcctg gagctgctcc cggcctgccc
     7561 tccaggagct tctgcctcaa ggaccagtgt tttcaatccc tctgaagcgc catacacggc
     7621 ggcggcaagt cccgccatgc ccgcgccggc taccaccaga tcgtagaagt cctgcttcgc
     7681 ctgggtgcgc aggcccacct tttgggccag ctcatcctcc tgcggctgta cgagcgacgt
     7741 cccgtcctga aacagaacaa cgggcagttt gctgtcgccc aggttaaacc gttgcagcat
     7801 ctccacggcc tgatcgtcgc gctcaggatc gagccagacg tagggcacct ggttccgcga
     7861 caggaaattc cgcacgcgat agtcgagcag cccgtagcgt ggaccaacga ctcgaatgcc
     7921 ctcaaagggc ggcttgaacc cctcgttcca gtcttccagc aggtcgtcca gcaccgggta
     7981 gaggcgctct tccggcggat cccacggctt ggtcaggtag tagtggatgc gcgccgtgtt
     8041 gatggcgcgg atcgccgcct cggtatcggc gtaggcggtc agcaggacgc ggcgagcctg
     8101 cggatactgc tcctgcacgc gctccagcaa ttccacaccg gccatgccgg gcatccgctg
     8161 gtcgctcacg atcagggcca cgacatcgcc gcggtccttg agctgggcgc aggtgtccag
     8221 agccgcctgg cccgaaccgg cgcgcaggat ccggtagctc tgtccatatt tgcgacgcag
     8281 gtcctgcacc acggcttcca gcacgctggc gtcgtcgtcc acggcgagga tgatgggcct
     8341 ggccattcgc ttctgctcct gggtttctga agctcctcta ctaaattaca ggcgcgtggc
     8401 ctttcccgcg aaacgcggcg gcccggtcgg tccatcgcca cttgcctcag aaacggggca
     8461 aaccgagggc caaatcgcct ccctggatgc ggaaccgccg cccacaggcg tgaccgccgt
     8521 cacaggactt tggagtccca gcgggttact ttttgtcctg aatgatggca gcacccaggt
     8581 catcgatgaa gccctttccg gcggcccacc accggaaagc tgcatcgttc aaccgacgcc
     8641 ctgtcgtggc aactcgatgc attcaccaag aaatgcacgg gcggatcctg ttcctcggaa
     8701 acgatgaccg tcttttcgca cgttctgttt tgggacgtgc ttgcacccac aaagtcttcg
     8761 ggccctatcc cgtcgacgca cgattttgag gaagcggaac tatgaacaag ctgatccttg
     8821 ctgataacca ggccatctac cgagccggaa ccgccaaaat tctggcgatg gaagatgact
     8881 tccgcatcat tgcccagtgc gccgaggccg accgcctctt tcccgccatc gacaccttcc
     8941 gcggtgcgat tctcgtcttc gcctccgcgc tgaagctcga cccccagacc ctggtgccgc
     9001 gcgtggccgc cgccggcagc cgcagcatcg tcatcgccga aaacggcgaa tcggtgcagg
     9061 cgtggtttaa ctccggcgtt cagggagctg cccatcgtaa cgtgaccggc cctgccctga
     9121 tcgactgcgt ccggcatgtc ggcgccggcg accgcaatgt ccgtgctgcc ggcgtggatc
     9181 tgcccgtgac cgaagacgac agcgtcggct ctcgcgtccg cgaccgtctc acgcccaagg
     9241 aactgaagat cgtcgcgttg attgtccagg gctgcaagaa caaggagatc gccgtccgcc
     9301 tgaacaccac cgagcaggtc atcaagaact acctgcgcag tgtttacgac aaaaccggcg
     9361 tctcggatcg cctggagctg gc
//
LOCUS       DASZYN010000570        10294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_348316, whole genome shotgun sequence.
ACCESSION   DASZYN010000570 DASZYN010000000
VERSION     DASZYN010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10294
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348316"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VHE33_14790"
     CDS             <1..494
                     /locus_tag="VHE33_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98768.1"
                     /translation="HPFEDGNGRIARAIADMILARSEHSPQRFYSMSAQIRQERKAYY
                     EILEATQRGDLDITPWLEWFLACLGRAFDRAETILAAVLHKARFWERFAAVSFNDRQR
                     KIVNRLLNGFEGKLTSSKWAKLARTSQDTALRDIEDLIRKKVLLRDSAGGRSTSYSLA
                     PLK"
     gene            622..1197
                     /locus_tag="VHE33_14795"
     CDS             622..1197
                     /locus_tag="VHE33_14795"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HVT98769.1"
                     /translation="MSKSRLEAFSDGVFAVIITIMVLEMKVPEHTTFAALRSVLPVFL
                     TYVVSFIYIGIYWSNHHHLLHACERVSGAILWANLHLLFWLSLVPFATAWVSISIDAP
                     APVALYGAILLCAGTAYYILEQRLICLHGEDSRLARSVGKDRKGIASVILYAAAIAVA
                     FRHPRFACCVYVLVAVLWLIPDKRIERNLHE"
     gene            complement(1259..2026)
                     /locus_tag="VHE33_14800"
     CDS             complement(1259..2026)
                     /locus_tag="VHE33_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98770.1"
                     /translation="MVRAEEPEREVSFGSSQSVIFREEEGGGHGNFLASSYARIVANP
                     AWKQRLTKSYTASRYVPRAADRKRYELECASSSDALLMNVFCYPGVLRNARVCALLGI
                     EAGLRPEFGFRPEIPLKNGKCDRTEMDMRLGALLVEAKLTEGDFQRAPVRLVERYRDL
                     EEAFDMDRLPVRDGVVRSYQLIRCVLAAQAWGGSFVVICDGRRPDLAERWFEVMCAVK
                     DAALRTRLGLLTWQEIAAGLPARVQRFLEEKYGIRTE"
     gene            complement(2104..2685)
                     /locus_tag="VHE33_14805"
     CDS             complement(2104..2685)
                     /locus_tag="VHE33_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98771.1"
                     /translation="MASDGNSLLFSMGAMGQFLGEIAFWVTDVTVLSSVLALIEIVIE
                     KDRGWASAFDEVGWGRKLLAGTAVVRWIDKPYVTSYHFLVFGALLPIVLWAQYRIGIV
                     HRFAASSHSDHPIADLLFFFSAFLAICILEDFLWFVLNWHYPSSLTDLLAGDVWWHTR
                     WIPLGPSVKLPRCYVSVGGAALCLLAISIALSR"
     gene            complement(2740..4209)
                     /locus_tag="VHE33_14810"
     CDS             complement(2740..4209)
                     /locus_tag="VHE33_14810"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HVT98772.1"
                     /translation="METTAPNSANDTDVLIVGAGPVGLFLANECARRGLRWRLIEERA
                     GQSEHSKALAIFPRTLEIFDMADVVGPFLRKANRVTRVEVTSHERTLTELPFEPEPTP
                     YSFVAMVPQNVTEAILANDLLERGGTIEYETAFVSAQERDGGVEARVDRKGESAVVTA
                     SFVVGCDGARSKVRHGLGVELEGGEYRPEFMLADIETNAFRTASELLLCPSELGPLAL
                     FPMDATRWRVVATVEKPEGQAPSLELTQRVLRERAPEGIEATELHWSSYFRIHHRHAK
                     QLRVGRMFIAGDAAHLHSPFGGQGMNTGLHDAWNLAWKLDLFLRGHGNETLLDSYAAE
                     RLPAIRSVVEMTDLMTKGLGTTNKLAQVVRDAVIPTVSHLGLFQHAFVKRLSGLGIAY
                     GGSPIVEGSGERYFDESLRGGGIGSRFLLMLGGDVSAEDDGVKMLRAAFAEVLEVRAG
                     EAPGIRLVRPDGYVAYAARRTNGARTWEALGALLEKQTR"
     gene            4294..5262
                     /locus_tag="VHE33_14815"
     CDS             4294..5262
                     /locus_tag="VHE33_14815"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HVT98773.1"
                     /translation="MSLRTARVIATVSTTAPATHPPAALRRFAWGVLAYNVAVIVWGS
                     AVRATGSGAGCGDHWPLCNGTVVQHHPGLATLIELAHRATSGIDLLCVLALLFWTFYS
                     VPRRHLARAFAVAVLVLTLNEAFLGALLVLLGHTANDTSPSRAVYLGLHLTNTLLLLA
                     ALALTAHFLSRRTAVMRGIVALRTARLAITGLIVTLIVGVTGSLAALGDTLYPAHDLL
                     NAIAQDFSPGSSWLLQIRWLHPAISLIAGAFIVWLIARSVRSPHRPLAYAVLALLALQ
                     YLLGIGDILLLAPTWMQMLHLLGADLLWIALVVLSARLCLVARAGP"
     gene            complement(5490..6683)
                     /locus_tag="VHE33_14820"
     CDS             complement(5490..6683)
                     /locus_tag="VHE33_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98774.1"
                     /translation="MAGVFALFGEDARAEPYVSRTPNLRRRIRRLLEPRPDQTKRLQL
                     AGRVARIEYVPTGSDFEALLRLYEASWTVFGERARKRLHLRPPALLRMTVENAYPRVY
                     VTNRVTKGGMEDLYGPFPSRVAAERFCEDMLDLFKLRRCVDDLHPDPAFPGCVYSEMK
                     KCLAPCFRGCTDERYAEEAAAVRTFLRTRGQSVVEALKAERDAASGLMEFERAAEIHS
                     RLARAEVVAAEAPEAVHALAHLDGVIVQASAEAEHVELFLLKCGSLRGPALFSVVGIR
                     HPNEAAGSSSLFAHPTPNQEPVPLEGSVVTTLATRDELERRLESALSELSPPEARVGA
                     RGKNRSGNIADYLCLFSRWYYRPQAKRAGEIVFAGEDGRASPKAVLRAVSRVWVKSQT
                     VTGSR"
     gene            complement(6835..8217)
                     /locus_tag="VHE33_14825"
     CDS             complement(6835..8217)
                     /locus_tag="VHE33_14825"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HVT98775.1"
                     /translation="MMSAVDTKTNLQELAEFVVARALLNGASDAEVTIRQGDEFSTTV
                     RMGEVETLKESGSRGVGLRVLMKADDGYRVASTSSSDFTPDGIEYLVQGALGLAQVTS
                     VDPFAGLAEEGEFGKLSGDLGLYHEDVYSLPIEERIAWARRAEAAAMSSDTRIVNSDG
                     ASFDATTGRRVFANSRGFAGEYQWSYCSIAASPIAQGVNGEMQRDYWYSQARSFRGLE
                     QPEEVGLEAARRTLRMLDARRVPTQEAPVIFAPEVARGLVGAVFDAAAGDAIYREASI
                     FAGKLGDTVAAESVTVVDDGTMPGGFGTSPFDAEGLPSRRTVIVERGVLRNYILNTYA
                     GRKLGMKSTGNAGRGLAGNPYLGAGNLFLQAGMRTAEEILQGVERGLYVTRMLGQGVN
                     LVTGDYSRGAAGLWIENGELAYPVHEITIAGNLKEMLRNIRAIGSDLVFRGSTAAPTL
                     RVDGMTIAGS"
     gene            complement(8279..9709)
                     /gene="tldD"
                     /locus_tag="VHE33_14830"
     CDS             complement(8279..9709)
                     /gene="tldD"
                     /locus_tag="VHE33_14830"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF008006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HVT98776.1"
                     /translation="MEHKRYFFEKFGITERLLERCLGEALSAGGDYADLYFESVAATS
                     LGLDEQIVKSASQGVSAGCGVRVVSGERTGYAYTDDLSAERLLRAARTAALIASGPAK
                     QPVQGFTETETPNLYPAAGAELDADLAVKLDLARRADRAARAYDPRITQVRASYADEL
                     RRILIAASDGTYASDTQPLARMSVFVIAKDGERTSKGSSGGGGRVEIEYFTGAKGPEH
                     FAEEAARTAILQLGAVAAPAGEMEVVLGPGWPGVLLHEAVGHGLEADFNRKKTSAFAG
                     LVGRPVASSKVTVVDNGRMPSRRGSLNVDDEGSATQETVLIENGVLKGYLSDKLSARL
                     MGIPSTGNGRRESYQQIPMPRMTNTYMLAGEEAPEDIIRSVKRGLYAVNFGGGQVDIT
                     SGKFVFSASEAYLIEDGKVTAPVRDATLIGNGPEALKHVSMVGNDLALDEGIGTCGKD
                     GQSVPVGVGMPTVKLDRMTVGGTGGR"
     gene            9867..10019
                     /locus_tag="VHE33_14835"
     CDS             9867..10019
                     /locus_tag="VHE33_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98777.1"
                     /translation="MKTLLLLGCLLALSAPAFAASHHHKSPKQHYKHHHVKVRNGTPN
                     HRRAGT"
BASE COUNT         1867 a         3962 c         2493 g         1972 t
ORIGIN      
        1 tccatccgtt cgaggacggc aatggccgca tcgcgcgcgc catcgcggat atgattctcg
       61 cccgctccga gcacagcccg cagcgcttct acagcatgtc ggcgcagatc cggcaggaac
      121 gcaaggctta ttacgagatc ctcgaagcca cgcagagagg cgatctcgac atcacgccct
      181 ggctggagtg gttcctcgcc tgcctggggc gcgccttcga tcgcgccgaa accattctgg
      241 cagccgtgct gcacaaggcg cgcttctggg agcgtttcgc cgcagtgtcc ttcaatgacc
      301 ggcagcggaa gatcgtcaac cggctgctca atggcttcga gggcaagctg acctcatcga
      361 aatgggccaa actcgcgcgc acctcgcagg acaccgctct gcgcgacatc gaggacctca
      421 tacggaaaaa ggtgctgctc agggattcag ccggcggacg aagcacctcc tactcgcttg
      481 cgccccttaa atgaaccaac gtttccctcc atccgcctcc cgtctcgtgc ggtgaataca
      541 gcctctaatc tccgcatatt atgcggtgaa taaagcctct tgtttccccg ccgcgatagg
      601 atggcccgga ggaaaagcca catgagcaag agccggctcg aagccttcag cgacggcgtc
      661 ttcgccgtca tcatcaccat catggtgctc gagatgaagg tccccgagca caccaccttc
      721 gcagccctgc gctccgttct gcccgtcttt ctcacctatg tggtcagctt catctacatc
      781 ggcatctact ggagcaacca ccatcatcta ttgcacgcct gcgaacgcgt cagcggcgcc
      841 atcctctggg ccaatctgca cctgctcttc tggctctccc tcgttccctt cgccacggcc
      901 tgggtcagca tcagcatcga tgctccagcc cctgtcgccc tctacggcgc cattctcctc
      961 tgcgcgggca cggcctacta cattctcgaa cagcggctca tctgccttca tggcgaagac
     1021 tctcgcctcg cccgatcggt cggcaaagac cggaagggaa tcgcgagtgt cattctctac
     1081 gcagcggcta tcgccgtcgc attccggcat ccccgcttcg cctgctgcgt ttatgtgctg
     1141 gttgctgtcc tctggctgat ccccgacaag cgcatcgaga ggaatctcca cgagtgatgc
     1201 gcatcagtcc accgccactg gctgcactga atcccatccg ccgcatggag gtcgaacctc
     1261 attcggttct gattccgtac ttctcctcca gaaatcgctg cacccgcgcc ggcagtcccg
     1321 ccgcaatctc ctgccaggtc aacagcccca gccgggttcg caacgccgca tccttcaccg
     1381 cgcacatcac ttcgaaccat cgctccgcca gatccggccg ccgcccatcg cagatcacca
     1441 caaaggatcc gccccacgcc tgcgccgcga gcacacaccg gatcagctga tacgaccgca
     1501 ctacgccgtc ccgcaccggc agacgatcca tatcgaacgc ctcttccaga tcgcgatacc
     1561 gctccaccag ccgcaccggc gctcgctgga aatctccctc cgtcagcttt gcctcgacga
     1621 gcagtgcgcc cagccgcatg tccatctccg tccggtcaca cttgccgttc ttcagtggaa
     1681 tctccggccg aaacccaaac tccggccgca atccagcctc gatccccaac agcgcacaca
     1741 cgcgcgcgtt ccgcagcacg cccggatagc agaacacatt catcagcaac gcatcggaac
     1801 tgctcgcgca ctccagttca taccgtttgc gatccgcggc ccgcggaaca taacggctag
     1861 cggtgtagct tttcgtcagc cgctgcttcc atgcgggatt cgccacgatc cgcgcatacg
     1921 agctggcgag aaaattccca tgccctccgc cctcctcttc gcggaagatc acgctctgcg
     1981 acgagccgaa ggaaacttcg cgctccggtt cctccgctcg taccacgttc cgagcgctga
     2041 tctctcgccg cagctcggcc gagctcagtg ctcgtctctc tgacgaatct ctgctcatcg
     2101 ccgctaccgt gacaacgcga tgctgatggc cagcaggcag agagccgccc ctcccaccga
     2161 gacgtagcag cgaggaagct tcacggaagg tcccagggga atccagcgcg tatgccacca
     2221 aacgtctccc gcgagcaggt ccgtcaagct gctggggtaa tgccagttga ggacaaacca
     2281 aagaaagtct tcaaggatgc aaatcgccag aaacgccgaa aagaagaaca ggaggtctgc
     2341 gatggggtga tcgctgtgcg acgatgccgc gaacctgtgg acgatgccga tccggtactg
     2401 agcccacagc acgatgggca ggagcgctcc gaagaccagg aaatgatacg aggtgacata
     2461 cggcttatcg atccatcgga ccacggcggt tccggccaag agcttcctgc cccaacccac
     2521 ctcgtcgaaa gccgacgccc atcctcgatc tttctcgatc acgatctcaa tcagggccag
     2581 aacggagctc aggaccgtaa cgtccgtgac ccaaaacgcg atttccccta agaattgccc
     2641 catagcgccc atagagaata acagcgagtt tccgtcagag gccattgcga ttccgcagcg
     2701 tccggaaatt ctgctgaaga attcgcgcca gcgatagcgt cagcgcgtct gcttctccag
     2761 cagcgccccc agcgcctccc acgtgcgcgc accattcgtg cgccgcgccg catacgccac
     2821 atacccatcc ggccgcacca gtcgaatccc cggcgcctct cccgcccgca cttccagcac
     2881 ctccgcgaac gccgcacgca gcatcttcac tccgtcgtcc tccgcgctca catcgccgcc
     2941 cagcatcagc aaaaaccggc tcccgattcc gccgccgcgc agcgactcat cgaaataccg
     3001 ttctcccgat ccctccacga tcgggctccc tccatacgcg atccccagcc ccgacaatct
     3061 cttcacaaaa gcgtgctgga acagtcccag gtgcgagacc gtcgggatca ccgcgtcgcg
     3121 caccacctgc gccagcttgt tcgtcgtccc cagccccttc gtcatcagat ccgtcatctc
     3181 caccacgctg cggatcgccg gcagtcgctc agccgcatag ctgtcgagca gcgtctcgtt
     3241 gccgtgcccg cgcaggaaaa gatccagctt ccacgccagg ttccacgcat cgtgcagccc
     3301 cgtattcatc ccctgcccgc caaacgggct gtgaagatgc gccgcatcgc ctgcgatgaa
     3361 catccgcccc acgcgcaact gttttgcatg gcgatgatgg attcggaaat agctgctcca
     3421 gtgcagttcc gtcgcttcga ttccctcagg ggcccgttcc cgcaggaccc tctgcgtcag
     3481 ttccaggctc ggcgcctgcc cctccggttt ctcgacggtc gccaccactc gccaccgcgt
     3541 cgcgtccatg gggaacaacg ccagcggtcc caactcgctg gggcacagca gcaactcgct
     3601 cgcagtgcgg aacgcattcg tctcaatgtc cgccagcatg aattcggggc gatattcccc
     3661 gccctcaagc tccaccccga gcccgtgccg aaccttgctg cgcgcgccat cgcagcccac
     3721 cacgaacgac gccgtcacca ccgcagactc tcccttgcga tcgacccttg cctccacacc
     3781 cccgtccctc tcctgcgccg agacgaacgc cgtctcatac tcgatcgtcc cgcctcgttc
     3841 cagaagatcg ttcgccagga tggcttccgt cacgttctgc ggcaccatgg cgacaaacga
     3901 atacggcgtt ggctcgggct cgaacggcag ctcggtcagc gtcctttcat gtgacgttac
     3961 ttccactcgc gtgacacggt tcgccttccg cagaaacggc ccgacaacgt cggccatgtc
     4021 gaagatctcc agcgtccggg gaaaaatcgc cagtgccttg gaatgctctg actgtcctgc
     4081 cctctcctca atcagccgcc accgtagccc acgccgcgca cattcattcg ccagaaagag
     4141 cccaactgga ccggctccaa cgatcagcac atccgtatcg ttcgcactat tcggggccgt
     4201 tgtctccatt tccctttaga gcaccagacc tccggcagag tttcatcctc cgcgccactt
     4261 gtctgcactt ctcagcgtca ccacagacta gaattgagtc ttcggaccgc tcgcgtgatc
     4321 gccaccgtct ccaccaccgc tcccgccact cacccgccag cggcgctgcg ccgcttcgcg
     4381 tggggcgttc tcgcttacaa cgtcgctgtc atcgtctggg gttccgccgt tcgcgccacc
     4441 ggctccggcg ccggctgcgg cgatcactgg cccctctgca acggcaccgt tgtgcagcat
     4501 catccgggtc tcgctacact cattgaactc gctcaccgcg ccaccagcgg catcgacctc
     4561 ctctgcgttc tcgcgctcct gttctggacg ttctactccg tgccgcgccg ccacctcgcc
     4621 cgcgcctttg ccgttgccgt tctcgttctc accctgaacg aggccttcct cggcgcgctc
     4681 cttgtcctcc tcggccatac cgcgaacgac acctcgccgt cacgcgccgt ctacctcggc
     4741 ctgcacctca ccaacaccct cctcctcctc gccgctcttg ccctcaccgc tcacttcttg
     4801 tcccgccgca ccgccgtgat gcgtggaatc gtcgccctcc gtaccgcgcg ccttgcgatc
     4861 accggcctca tcgtcaccct catcgtcggc gtcaccgggt cactcgcggc cctcggcgac
     4921 acactctacc cggcacacga tctcctgaac gccatcgcgc aggacttctc ccccggcagc
     4981 tcctggctcc tccagattcg ctggctccat cccgccatca gcctcatcgc cggcgccttc
     5041 atcgtctggc tcatcgcccg cagcgtgcgc agcccgcacc gccctctcgc ttatgctgtc
     5101 ctcgcactcc tcgccctcca gtacctgctc ggcatcggcg acatcctgct cctcgcgcct
     5161 acctggatgc agatgcttca tctcctcggt gccgacctgc tctggatcgc tctcgtcgtc
     5221 ctctcagctc gcctctgtct ggtcgcccgc gccggccctt agaactcctg gaccgtatct
     5281 gtctcggaga tcaggtcaaa tcggcgaatc aatggctgcc ccggtgccct atgcgcgaaa
     5341 aaaccggaat ccaacgggtc ttcaaacgcc cctcaatcgt ctccacaggc attcattgat
     5401 ggacctccct ggtgccgaag attctccgtg atcttcggcg cccttttagt gggcagggct
     5461 gcactcgaag aaccatctga cgcaaccgac taccggctgc cagttactgt ttggcttttc
     5521 acccacaccc gcgaaaccgc ccgcaacacc gccttcggtg aagccctgcc atcctcgccc
     5581 gcaaacacaa tctcccccgc ccgtttcgcc tgaggccggt aataccagcg gctgaaaagg
     5641 cacagatagt ccgcgatgtt tccgctgcgg ttctttcccc tcgcgccaac acgagcctct
     5701 ggcggtgaca gctctgacag cgccgactcc agtcttcgct cgagttcatc gcgcgtcgcc
     5761 agcgttgtca cgaccgaacc ctcgagcgga accggctcct gattcggcgt cgggtgcgcg
     5821 aacagcgacg acgagccggc cgcttcgttc ggatgccgta tccccacaac cgagaaaagc
     5881 gccggtccgc gcagtgatcc acacttgagc aaaaacagct ccacatgctc cgcctccgcg
     5941 gacgcctgca cgatcacgcc atccaggtgc gcgagcgcat gcaccgcctc aggtgcctcg
     6001 gccgcaacga cttccgccct ggccagcctc gaatgaatct ccgccgcccg ctcaaactcc
     6061 atcaatcctg acgcggcatc gcgctccgcc ttgagtgcct ctaccacgct ctgcccgcgc
     6121 gtccgcaaaa acgtccgcac cgccgcagcc tcttcggcat aacgctcatc cgtgcatccc
     6181 cgaaaacacg gcgcgaggca ttttttcatc tccgaataca cacatcccgg aaatgccgga
     6241 tcgggatgca aatcgtcaac gcagcgccgg agcttaaaga ggtccagcat gtcttcgcag
     6301 aaccgctccg ccgccacccg cgacggaaat ggcccataca aatcctccat tccccctttg
     6361 gtaactcgat tcgttacata aacccgtgga tatgcattct ccaccgtcat ccgcagcaga
     6421 gccggcggtc tcagatggag tctcttccgc gccctctctc cgaacacggt ccacgaagcc
     6481 tcatacagtc gcaacagagc ctcgaaatcc gaacccgtcg gtacatactc gatccgcgcc
     6541 acgcgccccg ccaactgcaa tctctttgtc tgatccggcc ttggctccag caacctgcga
     6601 atccgccgtc gcagattggg cgtccggctt acatacggtt ccgctcgcgc gtcctcgcca
     6661 aacagcgcaa acacgcccgc caccgcggga atctcccccc acctcggctc actggcacta
     6721 aacggaattt ggttcggaaa catcaaggct gactctacga actatacgag cacgagaatg
     6781 cgccttcgtg cgaagccttt ctattccctg ctggctattc ccctggccgt gccttcaaga
     6841 tcccgcgatc gtcatcccat ccacccgcag cgtcggtgcc gccgttgacc cgcgaaacac
     6901 cagatcgctc ccgatcgcac gaatgttcct cagcatctcc ttcaggttgc ccgcaatcgt
     6961 gatctcgtgt acgggatagg ccagctcacc attctcaatc cacaatccgg ccgcgcctcg
     7021 cgaatagtcc cccgtcacca gattcacgcc ctgccccagc attcgcgtca cgtagagccc
     7081 tcgttccaca ccctgcagaa tctcctctgc agttcgcatc cccgcctgaa gaaacaaatt
     7141 tccggcgccc agatatggat ttcctgccaa acctcgtccc gcgttccccg tactcttcat
     7201 tcccagcttg cgtcccgcgt acgtgttcag gatgtagttc ctcaacacgc cccgctcgac
     7261 aatcaccgtt cgccggctcg gcagtccctc tgcatcgaac ggcgacgtgc cgaacccacc
     7321 cggcatcgtc ccatcatcca ccaccgtcac cgactcagcc gcgacggtat cacccagctt
     7381 gcctgcaaaa attgaagcct cgcgatagat cgcatcgccc gccgcggcgt caaataccgc
     7441 cccgaccagt ccgcgcgcta cctccggcgc gaagatcacc ggcgcctcct gcgtcggcac
     7501 cctgcgtgca tccagcattc gcagtgtccg ccgcgccgcc tccagcccca cctcttccgg
     7561 ttgttcgagg cctctgaacg accgcgcctg cgaataccag tagtcgcgct gcatctcccc
     7621 attcacgccc tgcgcaatag gactggccgc aatcgaacag tacgaccact ggtactcgcc
     7681 cgcaaaccct cgcgaattcg caaacactcg ccgcccggtc gtcgcgtcga agctcgcccc
     7741 atcgctgttc acgatccgcg tatcggagct catcgccgcc gcctctgccc gacgtgccca
     7801 cgcgattcgc tcctcaatgg gcagcgagta aacgtcctcg tgatagagac ccaggtcccc
     7861 gctcagcttc ccgaactcgc cctcctcagc aagccccgca aacggatcca cagaggtgac
     7921 ctgcgccaag cccagcgcac cctgcaccaa atactcgatt ccatccggcg taaagtcgct
     7981 cgacgaggtg ctcgccaccc gatacccgtc gtccgccttc atcagcaccc gcagccccac
     8041 gccgcgcgag ccggactcct tcagcgtctc cacctcgccc atccggaccg tggtcgagaa
     8101 ttcatccccc tgccggatcg tcacctcggc atcggaagcc ccgttcagaa gcgcccgcgc
     8161 cacgacgaac tctgccagct cctgcaaatt ggtttttgta tcgactgcgc tcatcatgtt
     8221 tttccaggag gcgcgaagca catccgctgg tctgtccagg atcgaccggc caggaattct
     8281 accttccccc cgtgcccccc accgtcatcc gatccagctt cactgtcggc atgcccacgc
     8341 ccaccggcac gctttgcccg tccttgccgc aggttccgat cccctcatcc agcgccagat
     8401 cgttccccac catcgacaca tgcttcagcg cttccggccc gtttccgatc aacgtcgcat
     8461 cccgtaccgg tgccgtcacc ttcccgtcct cgatcaggta tgcctccgac gccgagaaca
     8521 cgaatttgcc actcgtgatg tccacctgcc cgcccccgaa gttcaccgcg tacaacccgc
     8581 gcttcacgct gcggatgatg tcctccggcg cctcttcacc cgccaacata taggtattgg
     8641 tcatgcgcgg catcggaatc tgctggtagc tctccctccg cccgttgccc gtgctcggaa
     8701 tccccatcaa ccgcgcactg agcttgtcac tcagatatcc cttcaacacg ccgttttcaa
     8761 tcagcaccgt ttcctgcgtc gccgatccct catcgtccac gttcagcgat ccgcgccgcg
     8821 acggcatccg cccgttgtct accacggtca ccttgctgct cgccaccggc cgaccgacca
     8881 gaccggcaaa cgctgatgtc ttcttccggt tgaagtccgc ttcgagccca tgccccaccg
     8941 cctcatgcag caggacgccc ggccaccccg gccccaacac gacttccatc tctcctgccg
     9001 gcgccgccac cgcgcccagt tgcaaaatcg ccgttcgcgc cgcttcctca gcgaagtgtt
     9061 caggaccctt cgccccggtg aaatactcga tctctacccg gcctccgccg cccgagctcc
     9121 ccttcgacgt ccgctcgcca tcttttgcga tcacgaaaac gctcatccgc gccagcggct
     9181 gcgtgtcgct cgcataggtc ccatcggaag ccgcaatcag aatccgccgc agttcatccg
     9241 catagctcgc tcgcacctgg gtaatccgtg ggtcataggc ccgagccgcg cgatcggcgc
     9301 gccgcgccaa gtccagcttc accgccagat ccgcgtccag ctccgcccca gctgccggat
     9361 acagattcgg cgtctccgtt tccgtgaaac cctgcaccgg ctgcttcgcc ggtccgcttg
     9421 caatcagcgc cgctgtccgt gccgcccgca gcagccgctc cgccgacaag tcgtccgtgt
     9481 acgcgtatcc ggttcgctcg ccgctcacca cgcgtacgcc gcatcccgcg ctcaccccct
     9541 ggcttgccga cttcacgatc tgctcatcga gccccagcga tgtcgctgcc accgactcga
     9601 agtacaaatc tgcataatcg ccccccgccg acaatgcctc gcccaggcac cgctccagca
     9661 agcgctccgt gatcccaaac ttctcaaaga aataccgctt atgctccacg cttaaccctc
     9721 gtgttccatg atggcgttcg gaacttgtca cgcgaccgcc gccgtgcagc tcttatctct
     9781 acactaaccc tttcgttgca tgcccttcct gattcttgat cagaattccg gcgacagtcg
     9841 acccaccttc gtgccatgct cagtttatga agaccctcct cctcctcggg tgtctgctcg
     9901 ccttgtccgc gccggccttc gccgcctccc atcatcacaa gagtcccaag caacactaca
     9961 aacaccatca tgtgaaggtc cgcaacggca cgccgaacca ccgtcgcgcg ggtacatagg
    10021 cctggggtct gctgcctgga gcctggtgcg cagggctgtg ggcactgctc tgcacgacgt
    10081 gcgccgcagt gcagagtctt gtctttgtcg ccgccagcga accgcagagc agctctcaaa
    10141 cttcgtgaaa gctgccccac ttcttcgatc ttgttcagcg cacggtgagg acgagcacct
    10201 caacgctgta cggcggcact cgatggtgga tgttcgccga tacaccgctg agcgtgctgt
    10261 ctaccggcga gatggctttc ggctggtcga tcgt
//
LOCUS       DASZYN010000571         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_89366, whole genome shotgun sequence.
ACCESSION   DASZYN010000571 DASZYN010000000
VERSION     DASZYN010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89366"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1551
                     /locus_tag="VHE33_14840"
     CDS             <1..1551
                     /locus_tag="VHE33_14840"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HVT98778.1"
                     /translation="AHQGIRLAETLIDPLLLMVRTFGLHLHTLDIRQHARIHRKALEE
                     SSAWCAQNGAATPTALSPETRDVVETFRVIGEIKKGCSPEAIRQYVISGASSVEDVTA
                     VLWLARLGGVEVDGRGADPGLMPVPLFESIEDLRNAPRICKELWTSPGYAKLLASWNN
                     TQEVMLGYSDSNKDGGMLTSTWEIFCAHRALHEVAHDCGIRLRLFHGRGGTVGRGGGP
                     THRSIYAQPIGAFDGEIRITEQGEVLNWKYSDVVLAERNLELMIAASLDALARPNALL
                     PNERYSGVLKPSWEAAFGELSEASFGFYRERILQDPEVLHYFEVATPVSELEHARIGS
                     RPARRPGQISFANLRAIPWVFGWTQSRLLVPAWFGVGTAFSAFTKKPGGLELLREMMG
                     DFPLFTDVVRNVEMALAKADLGIASLYASLVPDAALRERVFGMLKSEFERTREAILAV
                     TGQQDLLEANPVLARSIVLRNPYVDPMSLIQVDLLRRKRAGEDTDEVNRAIAGTISGI
                     AAGLRNTG"
     gene            1708..>2113
                     /locus_tag="VHE33_14845"
     CDS             1708..>2113
                     /locus_tag="VHE33_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98779.1"
                     /translation="MQAGPPPEDLHAILSRFHTWAGKNPGNGNGKTKGAAEEGIREIP
                     YEEAMRLHREREVARGTRRTATPGKKKETAATQAAGKVSDTGSPNPSESQEDLPLWVR
                     NLSVVPDTEPFIELGATADAVAEQERTAVFDPP"
BASE COUNT          380 a          542 c          788 g          403 t
ORIGIN      
        1 gcgcaccagg gcattcgcct ggcggagacg ctgatcgatc cgctgcttct tatggtgcgg
       61 acgtttgggc tgcatttgca cacgctggat atccggcagc atgcgcggat tcatcgcaag
      121 gcgctggagg aatcgtcggc ctggtgtgcg cagaacggcg cggcgacacc gacggcgctg
      181 agcccggaga cacgggatgt ggtcgagacg tttcgggtga tcggcgagat caagaaaggc
      241 tgcagtccgg aggcgatccg gcagtacgtc atcagcgggg cttcgtcggt tgaggatgtg
      301 acagcggtgc tgtggctggc gcggctggga ggggtcgagg tcgacgggcg cggcgctgat
      361 ccgggattga tgccggtgcc gttgtttgag tcgattgaag acctgcgcaa tgcgccgcgc
      421 atttgcaaag agctgtggac gtcgccgggg tacgcgaagc tgctggcgtc gtggaataac
      481 acgcaggaag tgatgctggg ctactccgac tcgaacaaag acggggggat gctgacgagc
      541 acgtgggaga ttttctgtgc gcatcgggcg ctgcatgagg tggcgcacga ttgtgggatt
      601 cggctgcggc tgttccacgg gcggggcggg acggtggggc gcggcggggg gccgacgcat
      661 cgatcgatct acgcgcagcc catcggggcc tttgacgggg agattcgcat caccgagcag
      721 ggcgaggttc tgaactggaa gtattccgat gtggtgctgg cggagcgcaa tctggagctg
      781 atgattgccg cgtcactcga tgcgctggcg cggccgaatg cgctgctgcc gaatgaacgg
      841 tattccgggg tgctgaagcc gtcgtgggag gcggcgtttg gcgaactgtc tgaggcttcg
      901 tttgggttct atcgcgaacg gatcctgcag gatccggagg tgctccacta ctttgaagtc
      961 gcgacgccgg tgagcgaact ggagcatgcc cgtattggct cgcgcccggc gcggcggccg
     1021 ggacagattt cgtttgcgaa tctgcgggcg attccgtggg tgtttgggtg gacgcagagc
     1081 cgtctgctgg tgcctgcgtg gtttggagtg gggacggcct tctcagcatt cacgaagaag
     1141 cccggcgggc tggaactgct gcgggaaatg atgggcgact ttccgctgtt caccgatgtg
     1201 gttcggaatg tggagatggc gttggcgaag gcggatctgg gcatcgcgtc gctctatgct
     1261 tcgctggttc cggatgcggc gctgcgcgag cgggttttcg gcatgctgaa aagcgaattc
     1321 gagcgcacgc gtgaggcgat tctggcggtg accggacaac aggatctgct ggaggcgaat
     1381 ccggtgctgg cgcggtccat cgtgctgcgg aatccgtatg tcgacccgat gagcctgatc
     1441 caggtggatc tgctgcggcg gaagcgggcg ggtgaagata ccgatgaggt gaaccgtgcg
     1501 atcgccggaa cgatcagcgg gattgctgcg gggctgcgca acacgggttg atcgtcaagg
     1561 gcgactggaa tcggaagtaa cgtcgcgttc caaaatggaa aactcgacgg cggttgttac
     1621 caatgcggga atgcgcctga gaaaaccttc atctttctat tccggcttcc tgctgatgct
     1681 tttaggctcg gggtggagga agtcgtcatg caggccggac cgccccccga ggaccttcac
     1741 gccattctga gccgctttca tacctgggcc gggaagaacc cggggaatgg aaacgggaaa
     1801 acgaaggggg ctgctgagga agggatccgc gagattccgt acgaggaagc catgcgcctg
     1861 caccgcgagc gcgaggttgc gcgtgggacg cgccgcacag caacgccagg gaagaagaag
     1921 gagacagctg caactcaggc ggccggcaaa gtatcggaca caggctcgcc gaatccttca
     1981 gagtcgcaag aggacttgcc gttgtgggtc aggaacctgt ctgtggtgcc ggataccgag
     2041 ccgttcatcg aactgggcgc cacggccgac gctgttgccg agcaggagcg tacggcggtc
     2101 ttcgatcctc cca
//
LOCUS       DASZYN010000572         2534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_89737, whole genome shotgun sequence.
ACCESSION   DASZYN010000572 DASZYN010000000
VERSION     DASZYN010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89737"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="VHE33_14850"
     CDS             complement(<1..1223)
                     /locus_tag="VHE33_14850"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVT98780.1"
                     /translation="MIRFLQKDTRLTRWLFGVIIGATCITMVIFLVPGIFNDTTTSND
                     TYATIRRGGFFGRFLPAQETIPVVDVQQAVRRMARGQQIPDMLMPIYMQQAGQSLVQQ
                     HILIEEARRLGITATDDDVRRFLHQGQLGQVLFPEGQYIGDARYAELISDQLQITVEK
                     FESSIKQQIMADRLRDLITGGITISDAEVRDAALKQDTKIKFQYAVLSADDLRKTINP
                     TDTELETFYKQNAAKYKDADPETRKIQYVNITDSDIPGGKPQITQAQIQQYYNQNQQQ
                     YHVDPQVKVRHILISVDPNGGPNADATAKAKAQGILDQLRKDNGKDFAELAKKNSDDP
                     GSKDQGGELGWIKHGVTVPEFDQTSFSQQPGQISGLVRTRFGYHIIQTEAKEDAHTKP
                     LDEVKQSIVDTLTKQ"
     gene            complement(1358..>2534)
                     /locus_tag="VHE33_14855"
     CDS             complement(1358..>2534)
                     /locus_tag="VHE33_14855"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HVT98781.1"
                     /translation="SERDGWRHAWLIDRATGQAACITKFDADIIHPVLLDDAHGFFYF
                     LASPDDPIRQYLYRSRLDGTGSPERITPQNESGSHTYDASPNGEWAIHSYSSVSHPPS
                     YELVRLASHQTARTLVDNDQLIAKENALDPEPIEFTDTAIPGGITLSTFILKPPHFDP
                     SKKYPMLVNVYSEPAGAMVRDGWHFTFDKAVAREGYVVVAFDNQGTPAPRGRAWRRAI
                     YEKIGILNARQQSQAITAFEKTHAYIDPSRVGMWGHSGGGSATLNEMFQYPGQVRAAV
                     SSSPVPDEALYDSIYQERYMGLPSTDPKGYHDGSPINHAEGLTGHLLIIHGSGDDNVH
                     FQGTERLVNRLIALGKTFDFMDYPNRTHALSEGPGTTVHVNALRMRFLEEYIPPGPR"
BASE COUNT          437 a          670 c          873 g          554 t
ORIGIN      
        1 ttttgtttgg tgagagtatc gacgatcgac tgcttcacct catcgagcgg cttggtgtgg
       61 gcgtcttctt tggcttcggt ctggatgatg tggtagccga agcgcgtgcg gacgaggccg
      121 gagatctggc cgggctgttg cgagaaggag gtctgatcga attccggcac cgtgacgccg
      181 tgcttgatcc agcccagctc gccgccctga tctttgctgc ccgggtcgtc ggagttcttt
      241 ttcgccagtt ccgcaaagtc tttgccgttg tccttgcgaa gctgatcgag aatgccctgc
      301 gctttggcct tggcggtggc atcggcgttg gggccgccat tgggatcgac cgagatgagg
      361 atgtggcgaa ccttcacctg cggatcgacg tggtactgct gctgattctg gttgtagtac
      421 tgctgaatct gcgcctgggt gatctgcggc ttaccgccgg ggatgtcgct gtcggtgata
      481 ttgacgtact gaattttgcg cgtctcggga tcagcgtcct tgtacttggc ggcattctgc
      541 ttgtagaagg tctcgagttc ggtgtcggtc ggattgatgg tcttgcgcag gtcgtcggct
      601 gacaggaccg cgtactggaa cttgattttc gtgtcctgct tcagtgccgc gtcgcggacc
      661 tcggcgtcgg agatggtgat gccgccggta atgaggtcgc gcagacgatc cgccatgatc
      721 tgctgcttga tggaggactc gaatttctca accgtgattt gcagttgatc cgagatcagt
      781 tccgcgtaac gggcatcgcc gatgtactgg ccctcgggaa agagcacctg gccgagttgg
      841 ccctggtgca ggaaccggcg aacgtcgtcg tcggtggcgg tgatgccgag gcggcgtgcc
      901 tcttcgatca ggatgtgctg ctgcaccagg gattggccgg cctgctgcat gtaaatcggc
      961 atgagcatgt cgggaatctg ctgaccgcgc gccatgcggc gcaccgcctg ctggacgtcg
     1021 acgacgggga tggtctcctg cgcgggcaga aagcgaccga agaagccgcc gcgccggatg
     1081 gtggcatagg tgtcgttcga ggtcgtggtg tcgttaaaga tgccgggcac cagaaaaatg
     1141 accatggtga tgcaggtggc gccgatgatg acgccgaaga gccagcgggt gaggcgagtg
     1201 tctttttgca ggaagcgaat catgcgatgg tgacagcctt ttctgtggca gcgctctccc
     1261 gcgccgggcc gttggactca cgggtacgga gaggcagatg tcctgagggc aggaactgct
     1321 aacagtatag ccggtaggcc gtgtctgggc ctgccggtta ccgcggtccc ggcggaatgt
     1381 actcctcaag gaaacgcatc cgcagggcat tcacgtggac cgtggtgcct gggccttcac
     1441 tgagggcatg cgtgcggttg ggatagtcca tgaaatcgaa ggtcttgccg agcgcaatca
     1501 gccggttcac cagccgctcc gtgccctgaa agtgaacgtt gtcatcgccg gagccgtgaa
     1561 tgatgagcag gtgaccggtg aggccttcag catggttgat gggggagccg tcgtggtacc
     1621 ccttcggatc cgtggaggga agccccatgt agcgctcctg gtagatggag tcgtacagcg
     1681 cctcatcggg caccggagac gaagagacgg ccgcccgcac ctggcctgga tactgaaaca
     1741 tctcgttgag cgtggccgag ccgccgccgc tgtgtcccca catgcccacg cgtgaggggt
     1801 cgatgtaagc gtgggtcttt tcaaacgccg tgatggcctg ggactgctgg cgcgcgttga
     1861 ggatcccaat tttctcgtag atggctctgc gccaggctcg tccccgcggg gccggcgtgc
     1921 cctggttgtc gaacgccacc acaacatagc cctcgcgcgc cacggcctta tcgaaagtga
     1981 aatgccatcc atcgcgcacc atggcgccgg ccggctcgct gtagacattg accagcatcg
     2041 ggtatttctt cgatgggtcg aaatgcggtg gcttgaggat gaaggtgctc agcgtaatgc
     2101 cgcccgggat tgcagtgtcg gtaaattcga tcggctcggg atcgagcgca ttctccttag
     2161 cgatgagttg gtcgttgtcg accagggtgc gcgccgtctg atgggacgcg aggcggacca
     2221 gttcatagct gggcggatgc gaaacactgg aatagctgtg aatggcccac tcgccattcg
     2281 gcgaagcgtc ataggtgtgg gagccggatt cgttctgcgg ggtgattcgc tcgggagatc
     2341 ctgtgccatc gaggcgggag cgataaagat actggcggat gggatcatcc ggtgatgcga
     2401 ggaagtagaa gaagccgtga gcgtcgtcga gcagaaccgg atggatgatg tcggcgtcga
     2461 atttggtgat gcaggccgct tgtcctgtag cgcgatcgat cagccaggca tgacgccagc
     2521 cgtcacgttc gctg
//
LOCUS       DASZYN010000573         8111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_89816, whole genome shotgun sequence.
ACCESSION   DASZYN010000573 DASZYN010000000
VERSION     DASZYN010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8111
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89816"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3665)
                     /gene="rpoB"
                     /locus_tag="VHE33_14860"
     CDS             complement(<1..3665)
                     /gene="rpoB"
                     /locus_tag="VHE33_14860"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HVT98782.1"
                     /translation="MRVTQVRDYSKRGDALPIPNLIDVQTAAFKRFLQADAPVEKRKN
                     EGLEALLREVFPIESYDGNMKLEYLSYDLGKPRYTQDECRALRLTYGMPFRVRVRLSR
                     KDKPDILDEAIYLGEIPIMTGGGEFIINGAERVIVSQLHRSPGVDFVIDSQEGDRPLH
                     ACRVIPERGSWIEMNVTKKDVMAVRIDQSAKIPATTFLRAMDEKYSTNEALLRAFYET
                     KTVPVGQLKGDFYAVGSIVDTETGEEIVKSGAMLGDAVSKIQASSIKKVEIIERVNDP
                     VILNTIQEDGTINYEQALLKIYGRLRPGNPPHFEKAKALFAEKFFDANRYRLGKVGRF
                     RINRKFEQNVPEDVMTLRAEDILGSLKYIMDLRAGKGYIDDIDHLGNRRLRTIDELAS
                     EELRKGFLKLRRTVQERMSLKDPNELTKIVELVNSKSISSSIEFFFGRSELSQVVDQT
                     NPLSQLTHERRLSALGPGGLNRKRAGFEVRDVHISHYGRICPIETPEGTNIGLIASLS
                     IYAGIDEYGFLITPYRVVEHNKVNNTTRYLRADEEMKSVVAPPEVVHIESGKVRQEMV
                     LARVNGELNQIKGGDVQYVDISPKQTVAISAALIPFLEHDDANRALMGSNMQRQAVPL
                     LVTDPPVVATGMEKHVPGNSGMVIKAERSGVVTFCDASRIVIDNSDEYALRKNAGLND
                     KTCLTQKPLVSLGQRVKKGQIIADGPACRNGELALGRNVLVAFMTYDGYNFEDAIVIS
                     EKLVKQDTFTSIHIEEFDIEIRETKLGREEFTRDIPNVSEKALKNLDDAGIVRIGTRV
                     GPGDILVGKVAPKSKTELSPEEKLLHAIFGRAGEDVKNDSLEVPAGTEGVVIGAQRFS
                     RRMHMNDDQKKQLQKDMKKYGEDMNRRAVLLFKQMADELNEVTGQTMVDPGTRQKVGK
                     SDIDEVILEQIQNFQLSWVKGSEESRKQAETIFRRYDARIKAIFEEKERKLEHMKRGD
                     ELPSGVLEMVKVYLATKRQLSVGDKMAGRHGNKGVIARIVPEEDMPFMDDGTPIDILL
                     NPLGVPSRMNVGQILETHLGWAAAILGFQAVTPVFDGAEESEIQAAVEEANKHVRARK
                     KELDDKKVAPPTNEIFVEMPPSFKVQLFDGRTGDPFEQRTTVGYIYMLKLHHLVDDKI
                     HARSTGPYSLITQQPLGGKARTGGQRFGEMEVWGLEAYGASYVLQELLTVKSDDVEGR
                     TKIYESMVKGTNTLEAGT"
     gene            4207..4803
                     /locus_tag="VHE33_14865"
     CDS             4207..4803
                     /locus_tag="VHE33_14865"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVT98783.1"
                     /translation="MQPLEQLTNFSGIAESGVLGQARALPVPGGAPLCPPAEQCAGIL
                     LEAVPQIMRTLHAVIHEISQPEITVAQFRALMFVHRREGVNLSAAAEFLGLRLASTSK
                     LVDHLVNRRLLTRAPDPTDRRRMILRLTARGEALLQEADDVARRRLAGMLNMLGGEQL
                     QVLREGLGLLHHSFVPCGERNLMGTATSTFLASDTRQT"
     gene            4800..6248
                     /locus_tag="VHE33_14870"
     CDS             4800..6248
                     /locus_tag="VHE33_14870"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVT98784.1"
                     /translation="MKYLMIFVLILVLAGVGGGLWYNHMKAANANPLADLSTAKVDRG
                     DLRLSVATNGVVASNKDVDIKVRASGEIIKLPVDISQEVKKGELLMELDTKDQEPPLK
                     EAQAQVDSDKAHVKSAQLNADLSAMQLKTETIRANANLASAKAQLADANSKAGRTEAL
                     YQKQLASKEDLETAQTTAAQMAANEQLCEAAVAELDQQKVTVDMRKQDIVTAQAALDQ
                     DQARLDLEQQAMDYTKVYSPIDGTVSDLTVQIGNIVQSSISNVSGGTAVMTISDLSHI
                     FVMASVDESDVGQVRLDQPVRITSDSYPGVEFTGKVVRIATKGVNTSNVVTFEVKIEV
                     TSPNKRLLKPVMTTNVEIIAAERDNVLMIPMLAFTRKKSDSSQQDASEMPATSSAATQ
                     QAEGADAGATGGRRRSAGRPQEGTVTVVRDDGTQEVRNVVVGMNDRASYEVISGLSEG
                     ETVLLNPVTDSRFKNNGRRGRSGASQLMRIGR"
     gene            6259..6981
                     /locus_tag="VHE33_14875"
     CDS             6259..6981
                     /locus_tag="VHE33_14875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT98785.1"
                     /translation="MTHSHDNGALLRATDLVKTYVLGDNIVNALNGVSIAIERGEMVA
                     IRGPSGSGKSTLMNILGCLDRPTSGTYVLAGDDVSRMSDNSLAEARNKKIGFVFQTFN
                     LLPRMNALENVELPLLYGGALNAKKKSMDALTRVGLGERYHHLPNQLSGGQRQRVAIA
                     RAIVTDPAIVLADEPTGALDTKTGEEILNLFKKLNDEGSTIIIVTHDLKVAEHCQREI
                     YIRDGKVVPSEVRNLSPVAEGV"
     gene            6985..>8111
                     /locus_tag="VHE33_14880"
     CDS             6985..>8111
                     /locus_tag="VHE33_14880"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT98786.1"
                     /translation="MLFWITLRSAIKSLWANKLRSFLAMLGIIIGVGAVIAMIAMGAG
                     AQKQVVSQIEAMGTNLLIVQPQYKRDAGVTSGTMQKLTLDDAMAIAQLPGVKEVAPVV
                     SSSTQVKYEGQNSPESIQGTSPDYLEIRNFEIDKGRMFTEEESDGLARVAVMGPLAVA
                     NLFGVQSPLGKQIKIKGINFTVVGVTKSKGGQGWFNPDDEIFVPYSTAMKILFGLDYV
                     KEIDVLAKDGVNLTTLSGQPPREPGYGPPREFDPDDQPPPPPPDSVTALLRKRHRIQT
                     DEIDDFRIQNQDDIIATRTAFIWTFRVLLFSIASISLLVGGIGIMNIMIVSVTERTRE
                     IGIRMAIGARRLQVLLQFLVESVLLSITGGGIGIVVGVLISL"
BASE COUNT         1535 a         2435 c         2591 g         1550 t
ORIGIN      
        1 gtgccggcct cgagggtgtt ggtgcctttg accatgctct cgtaaatctt cgtacgtcct
       61 tcgacgtcat cggatttaac ggtgagcagt tcctgcagca cgtacgacgc gccgtaggct
      121 tccaggcccc acacttccat ctcaccgaag cgctggccgc cggtgcgggc cttgccgccc
      181 aacggctgct gcgtgatcag cgagtaggga ccggtgctgc gggcgtgaat cttgtcatcc
      241 accaggtggt gcaacttcaa catatagatg tagcccaccg tggtgcgctg ctcgaacgga
      301 tcgccggtgc ggccgtcgaa aagctggacc ttaaacgagg gcggcatctc gacgaaaatc
      361 tcgttcgtcg gcggggccac cttcttgtca tccagctcct tcttccgagc ccgcacatgc
      421 ttgttggctt cctcgacggc ggcctggatt tcggattcct cggcgccatc gaagaccggc
      481 gtgaccgcct ggaagcccag gatcgcggcc gcccagccca ggtgcgtttc gagaatctgc
      541 ccgacgttca tacgcgacgg cacgcccagc gggttgagca ggatgtcgat cggcgtccca
      601 tcatccatga aaggcatgtc ttcttccggc acgatgcggg cgatcacacc cttgttgccg
      661 tggcggcccg ccatcttgtc gccgacggag agctggcgct tggtggcgag gtaaaccttg
      721 accatttcga gcacgccgga gggcagctcg tcgccgcgct tcatgtgctc gagcttgcgt
      781 tccttttcct cgaagatcgc tttgatgcgg gcgtcgtagc ggcggaagat cgtctcggcc
      841 tgcttgcgtg attcctcgga gcccttcacc caggaaagct ggaagttctg aatctgctcg
      901 aggatgacct cgtcgatgtc ggatttgccg accttctgcc gcgtgccggg atcgaccatc
      961 gtctgccccg tgacttcgtt gagctcatcg gccatctgct tgaagagcag gacggcgcgg
     1021 cggttcatgt cctcgccata cttcttcatg tccttctgaa gctgcttttt ctggtcgtcg
     1081 ttcatgtgca tgcggcggct gaagcgctgg gcgccgatga cgacgccctc ggtgccggcg
     1141 ggaacttcga gcgagtcgtt tttcacatct tcgccggcgc ggccgaagat cgcgtgcagc
     1201 agtttttctt cgggcgacag ttccgtcttg ctcttgggtg cgaccttgcc gacgaggata
     1261 tcgccggggc cgacgcgggt gccgatgcgg acgatgccgg cgtcatcgag attcttgagg
     1321 gccttctcgg agacgttggg gatgtcgcgg gtgaattcct cgcggccgag cttggtctcg
     1381 cgaatttcga tgtcgaattc ctcgatgtgg atcgaggtga aggtgtcttg tttcacgagc
     1441 ttttcgctga tgacgatggc atcttcaaag ttgtagccgt cgtaggtcat gaacgcgacc
     1501 agcacgttcc ggcccagggc cagttcgccg ttcctgcacg ccgggccgtc ggcgatgatc
     1561 tggcctttct tgacgcgctg gcccagcgag accagcggct tctgggtgag gcaggtcttg
     1621 tcgttaaggc cggcgttttt ccgcagcgcg tactcgtcgg agttgtcgat cacgatgcgc
     1681 gaagcatcgc agaaggtcac gacgccggag cgctcggcct tgatcaccat gccggagttg
     1741 ccgggcacgt gcttttccat gcctgtggcg acgaccgggg gatcggtcac cagcagcggc
     1801 accgcctggc gttgcatgtt cgaccccatc aacgcacggt tggcgtcatc gtgttcgagg
     1861 aaggggatga gggccgcgga gatggcgacg gtctgcttgg gcgagatatc cacgtactgg
     1921 acgtcgccgc ccttgatctg gttgagttcg ccgttgacgc gcgccagcac catttcctgg
     1981 cggaccttgc ccgattcgat gtgcacgacc tcgggagggg cgaccacgga tttcatttcc
     2041 tcgtccgccc ggaggtatcg ggtggtgttg ttaaccttgt tgtgctcgac gacgcgatag
     2101 ggggtgatga ggaagccgta ttcgtcgata cccgcataaa tcgacaagga cgcgatcagg
     2161 ccgatgttgg tgccttccgg cgtttcgatg gggcagatgc ggccatagtg cgaaatgtgc
     2221 acgtcgcgca cctcgaagcc cgcgcgcttg cggttcaacc cgccgggtcc cagggccgag
     2281 aggcggcgtt cgtgcgtgag ctgcgagagc gggttggtct ggtcgacgac ctgcgaaagt
     2341 tcgctgcggc cgaagaagaa ctcgatcgag ctgctgatcg acttcgagtt gaccagctcg
     2401 acgatcttcg tgagttcgtt ggggtccttg agcgacatgc gttcctgcac cgtgcggcgc
     2461 agcttcagga agcccttgcg gagttcttcc gaagccagtt cgtcgatcgt gcgcaagcgg
     2521 cggttgccca ggtggtcgat gtcgtcgatg taccccttgc ccgcacgcag gtccatgatg
     2581 tacttgagcg agccgaggat gtcttcggca cgcagcgtca tcacatcttc cggcacgttc
     2641 tgttcgaact tgcggttgat gcggaaccgg ccgaccttgc ccaggcggta acggttggcg
     2701 tcgaagaatt tctcggcgaa gagagctttg gctttttcga agtgcggcgg gttgcccggg
     2761 cgcaggcggc cataaatttt cagcagcgcc tgctcgtaat tgatcgtgcc atcttcctga
     2821 atggtgttga ggatgaccgg gtcgttgacc cgttcgatga tctccacctt cttgatggaa
     2881 gaggcctgga tcttggagac ggcgtcacca agcatggcgc cggacttgac gatttcctcg
     2941 ccggtctcag tgtccacgat ggagccaacg gcgtagaagt cgcccttaag ctggccgacg
     3001 gggacggtct tggtttcgta aaacgcccgc agcagcgctt cgttggtcga gtatttctcg
     3061 tccatggcgc ggaggaaggt ggtggcggga attttggccg actggtcgat gcggaccgcc
     3121 atgacgtcct tcttggtgac gttcatctcg atccagcttc cgcgctcggg gatcacgcgg
     3181 caggcgtgca gggggcgatc gccttcctgc gaatcgatga cgaagtcgac gccgggagag
     3241 cggtggagct ggctgacgat gacgcgttcg gcgccgttga tgatgaactc gccgccgccg
     3301 gtcatgatgg ggatttcgcc gaggtaaatc gcctcgtcca gaatgtcggg tttgtccttg
     3361 cgggaaaggc ggacgcgcac gcggaaaggc atgccgtagg tgaggcggag ggcccggcac
     3421 tcatcctggg tgtagcgcgg cttgcccagg tcgtaggaga gatactcgag cttcatgttc
     3481 ccgtcataag actcgatggg gaacacttcg cggaggaggg cttcgagacc ttcgttcttg
     3541 cgcttttcga ccggcgcgtc ggcctggaga aagcgtttga aggcagccgt ctggacatcg
     3601 atgaggttgg ggatcggtag ggcatcaccg cgtttggaat aatcacgcac ctgcgtaaca
     3661 cgcatagaaa cacctcagcc aggggttcgc ggaaaatcgc catcgccgtt gttttcagcg
     3721 gcggtcaggt tgtcggaaat gtcgaggagc aacaggaaaa aaaagggggg agggcaaaac
     3781 ttcgggcaaa atcattcata gtcccgcaat atagcggggc cgcccttgca aagcaagtgc
     3841 cgcaaacgca aaaacatcgc aactcctcgc cgcgcgccgc gcggctggga gtgcgttttc
     3901 aaatcgatca tttcccttcc aaacctgcta cacctcgtgc cagcccgacg ggggtcatta
     3961 tagcaggccc cataacagag gcgagggcag gctgaagaat gagcagccgg gggcccatcc
     4021 cggcgttgca ggccagcggc cgggaggtgc ttgcgaccgc gcgatgacac cggggaggat
     4081 gccttggtta gtcgtccttt gcaaattttt ataattattt gtcaataata aatttatgca
     4141 caaatgcgga aggttttggc atgccgggat gcaataatcc ctcctcgttt ccgttattct
     4201 tcgtccatgc aaccattaga gcagctaact aatttttccg gcatcgccga atcgggggtc
     4261 cttggccagg cccgagcgct gcccgttccc ggcggcgcac cgctgtgccc gcccgcggag
     4321 cagtgcgcgg ggatcctgct cgaagcggtg ccgcagatta tgcggacgct gcatgcggtg
     4381 atccatgaaa tttcgcagcc ggaaatcacc gttgcccagt ttcgcgcgtt gatgttcgtg
     4441 caccggcgcg aaggcgtaaa cctgtcggcg gcggcggaat tcctgggcct ccggctcgcg
     4501 tcgacctcga agctcgtgga ccatctggtg aatcggcgcc tgctgacaag ggccccggat
     4561 cccaccgatc gccggcggat gatcctgcgg ctgacggccc ggggcgaggc gctgctccag
     4621 gaagccgacg acgtggcccg gcgccgcctc gcgggcatgc tcaacatgct cggcggcgag
     4681 cagttgcagg tgctgcgcga ggggctgggg cttctgcatc attcttttgt gccttgcggc
     4741 gaacggaacc tcatgggcac ggcaaccagt acattcttgg caagcgacac gaggcagaca
     4801 tgaaatacct catgattttc gttctcatcc tggtgctggc gggggtcggc ggggggctgt
     4861 ggtacaacca catgaaggcg gctaacgcca atccgctggc ggacttgagc accgccaagg
     4921 tggatcgcgg ggatttgcgc ctgagcgtgg cgaccaatgg cgtcgtggcc agcaacaagg
     4981 acgtggacat caaggtgcgg gccagcggcg aaatcatcaa gctgcccgtg gacatcagcc
     5041 aggaagtgaa gaagggcgag ctgctgatgg agctggacac gaaggaccag gagccgccgc
     5101 tcaaggaagc gcaggcgcag gtggattcgg acaaggccca cgtgaaaagc gcgcagttga
     5161 acgccgacct ctcggcgatg cagttgaaga ccgagacgat ccgggcgaat gcgaacctgg
     5221 catcagccaa agcgcagctt gccgatgcga acagcaaggc cggccgcacg gaggcgctct
     5281 atcaaaagca gcttgcgagc aaggaggacc tggagacggc ccaaaccacc gcggcgcaga
     5341 tggccgcgaa cgaacagctt tgtgaagccg cagtggccga attggatcag cagaaggtca
     5401 ccgtcgacat gcgcaagcag gacatcgtga ccgcgcaggc ggccctcgat caggaccagg
     5461 cgcgactgga ccttgagcag caggcgatgg attacaccaa ggtctattcc cccatcgatg
     5521 gcacggtgtc ggaccttacc gtgcagatcg gcaatatcgt gcagtcctcg atttccaacg
     5581 tctccggcgg cacggcggtg atgaccattt ccgacctgag ccatatcttc gtcatggcca
     5641 gcgtggacga aagcgatgtc gggcaggtgc ggctcgacca gcccgtgcgg atcaccagcg
     5701 attcttatcc cggcgtggaa ttcaccggca aggtggtgcg gatcgcgacc aagggcgtca
     5761 acacgtccaa cgtggtgacc tttgaggtga agatcgaagt gaccagcccc aacaagcggc
     5821 tgctcaaacc ggtgatgacg accaacgtcg agatcatcgc cgccgagcgc gacaacgtgc
     5881 tcatgattcc catgctggcg ttcacacgga agaagagcga ttcgtcgcag caggacgcgt
     5941 cggaaatgcc cgcgaccagt tcggcggcga cgcagcaggc cgaaggcgcc gacgccggag
     6001 ccaccggggg ccggcggcgg agcgccggca ggccacagga gggcaccgtg acggtggtcc
     6061 gggacgatgg cacgcaggaa gtgcgcaacg tggtcgtggg gatgaacgac cgggcgtcct
     6121 atgaagtgat ctcgggcctc tcggaaggcg agacggtgct gctcaacccg gtcacggatt
     6181 cccggttcaa aaacaacggt cgtcgcggcc gcagcggcgc cagccaactg atgcgcattg
     6241 gccggtaaag gatcatccat gactcattcg cacgacaacg gcgccctgct tcgcgccacc
     6301 gacctcgtca agacctacgt gctcggcgac aacatcgtca acgccctcaa cggcgtttcc
     6361 atcgccatcg aaagaggcga gatggtcgcc atccgcgggc cctcgggctc gggcaaaagc
     6421 acgctgatga acatcctcgg atgcctcgac cgccccacct ccggcaccta cgtgcttgcc
     6481 ggcgacgacg tgagccgcat gagcgacaat tcgctggccg aggcgcgcaa caagaagatc
     6541 ggcttcgtat tccagacgtt caacctgctg ccgcgcatga acgccctgga gaacgtcgag
     6601 ctgcccctgc tttatggcgg cgcgctcaac gccaagaaaa aatccatgga cgcgctcacc
     6661 cgggtcggcc tgggcgaacg gtaccaccac cttcccaatc agctctccgg cggacagcgg
     6721 cagcgcgtgg ccatcgcccg cgccatcgtc accgatccgg cgatcgtgct cgccgacgaa
     6781 ccgaccgggg ccctggacac caaaaccggc gaagaaatcc tcaacctgtt caaaaagctc
     6841 aacgacgaag gcagcaccat catcatcgtg acccacgacc tcaaggtcgc cgagcactgc
     6901 cagcgcgaaa tctacatccg cgacggcaag gtggtgccca gcgaagtccg caacctttcc
     6961 cccgtagcag aaggggtcta aagcatgctc ttctggatca ccctgcgaag cgccatcaaa
     7021 agcctgtggg ccaacaagct gcgttcgttt ctggcgatgc tgggcatcat catcggcgtg
     7081 ggcgcggtga ttgccatgat cgccatgggg gcgggcgcgc agaagcaggt ggtctcgcag
     7141 attgaagcga tgggcacgaa cctgctgatc gtgcagccgc aatacaagcg cgacgcgggg
     7201 gtgacctcgg gcacgatgca gaagctgacg ctggacgacg cgatggcgat cgcgcaactg
     7261 cccggcgtga aggaagtggc cccggtggtc agctcgagca cgcaggtcaa atacgaaggc
     7321 cagaattccc ccgaaagcat tcagggcacg tcgccggact atctcgagat ccggaatttc
     7381 gagatcgaca aggggcggat gttcaccgaa gaggaatcgg acggcctggc gcgcgtggcg
     7441 gtcatggggc cgctggcggt ggcaaacctc ttcggcgtcc aaagccccct gggcaagcag
     7501 atcaagatca agggcatcaa cttcaccgtc gtgggcgtca ccaaaagcaa gggcggccag
     7561 gggtggttca accccgatga cgaaatcttc gtgccttact ccaccgccat gaaaatcctg
     7621 ttcggcctgg attacgtcaa ggaaatcgac gtgctggcca aggacggcgt gaatctcacc
     7681 accctttccg gccagcctcc gcgggagccc ggttacgggc cgcctcggga attcgatccc
     7741 gacgaccagc ccccgccgcc cccccccgat tccgtcaccg ccctgctccg caagcgccac
     7801 cgcatccaga ccgacgagat cgacgatttc cgcatccaga accaggacga catcatcgcc
     7861 acccgcacgg ccttcatctg gaccttccgc gtgctgctgt tctcgatcgc ctccatctcg
     7921 ctgctggtcg gcggcatcgg catcatgaac atcatgatcg tctcggtgac cgagcgcaca
     7981 cgcgagatcg gcatccgcat ggcgatcggc gcgcgccggc ttcaggtctt gctgcagttc
     8041 ctcgtcgagt cggtgctgct cagcataacc ggcggcggga tcgggatcgt cgtgggtgtc
     8101 ctcatatcac t
//
LOCUS       DASZYN010000574         4525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1185829, whole genome shotgun sequence.
ACCESSION   DASZYN010000574 DASZYN010000000
VERSION     DASZYN010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185829"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VHE33_14885"
     CDS             <1..274
                     /locus_tag="VHE33_14885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVT98787.1"
                     /translation="QRQRVAIARALVNNPSIILADEPTGNLDSKTGVEIMALFDDLQR
                     KGNTIVLVTHEADIAEYAHRVVHIRDGKIFSDQPSSRATKAAAVSG"
     gene            547..870
                     /locus_tag="VHE33_14890"
     CDS             547..870
                     /locus_tag="VHE33_14890"
                     /inference="COORDINATES: protein motif:HMM:NF021431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HVT98788.1"
                     /translation="MHVLAAKHLKDAAGRFPKAAKQLAAWRSIAKDKCWRSPDDVKDT
                     FADAEFAGDFVIFRIHQNKYRLVTSIHYPGGVNGSATDGHVWVRSFLSDKQYEDQANW
                     EKGVL"
     gene            870..1328
                     /locus_tag="VHE33_14895"
     CDS             870..1328
                     /locus_tag="VHE33_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98789.1"
                     /translation="MGKTLADPVEMIRRGAPHLIGSEDELAGYTRALFTLTSKPRPTV
                     AEQEAINLLTLLIEQYETVRYVVPKGSPVEVLRQLLKRNGLRQSNVATEFGCVANASM
                     VMRGERQFTRSHIARLSKRFRVSPAVFFDENSAIPERRNMGKRAARAKAQ"
     gene            1383..1856
                     /locus_tag="VHE33_14900"
     CDS             1383..1856
                     /locus_tag="VHE33_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98790.1"
                     /translation="MSKALPDPAEMVQHGAPRLIRSDEELTEYSRVLFALTSIPKPTS
                     AEQEAIGLLTLLIEHYENRRYPLPEARSVEVLRLLMERNGVSEGKLTPELGFQASASL
                     ILLGKRQLNRGHIARLSRRFNVSPAVFFDEASAIPLRNGVRIKKRRSRSARAKVR"
     gene            complement(1997..2266)
                     /locus_tag="VHE33_14905"
     CDS             complement(1997..2266)
                     /locus_tag="VHE33_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98791.1"
                     /translation="MAAVLGAALMLAPGYVSARAQSDNSAKQDMKDAGHDAKAGAKDV
                     GHGVKKGTKSAYHASKKGTKKAWHKTKNTTKGAVKGAKNGAKEPQ"
     gene            complement(2395..2862)
                     /locus_tag="VHE33_14910"
     CDS             complement(2395..2862)
                     /locus_tag="VHE33_14910"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HVT98792.1"
                     /translation="MAEKKNSAALKELIDGLQQDLSREYQAIIAYTVYSNVLKGAQWM
                     SIAGELRKHAGEELAHAMTIADQIDYLGAVPNATPKPVKLSEKAEEMLRFDLDNETET
                     IRQYRKRVRQAEAIGHYALAEQIRQIISQEQEHQHDLATALGEDVPNVLEEGA"
     gene            3029..4087
                     /locus_tag="VHE33_14915"
     CDS             3029..4087
                     /locus_tag="VHE33_14915"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HVT98793.1"
                     /translation="MPALHHLFNDHAEHVRAKITGIYGILLLFNGAAWLWAVLAFDHY
                     PVLLGTAFLAYSFGLRHAVDADHIAAIDNVTRKLMQEGKRPVAVGFMFSLGHSTVVIL
                     GSLGLAALALGLQKHLDHYRTIGGVIGTSVSSLFLLGIGLVNLIILRHVYVTFARVRR
                     GEPYVDEDFDLLLNNRGFLARIFRPVFAMIRRSWHMYPLGFLFGLGFDTASEIGLLGL
                     SAAEASRGLSLWSIAVFPVLFAAGMSLIDTTDNILMLGAYGWAYIKPIRKLYYNLTIT
                     SVSVVVAFVVGGIEALGLIGGHLHLQGVFWNFIDRLNSNFGVLGYCIIGIFALAWIVS
                     IAIYKLRRFEDLELTPES"
     gene            4112..4441
                     /locus_tag="VHE33_14920"
     CDS             4112..4441
                     /locus_tag="VHE33_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98794.1"
                     /translation="MIVFGLNSSDLESIAPVLEAALNVRLMPREGYFHGGNYYSAETA
                     GGTIRLQYNRDIFSGKIGPIVRDWPVDRLVLSFEGSDDTSWELYLSALSQLSDLQPTR
                     LAPQSAG"
BASE COUNT          864 a         1603 c         1108 g          950 t
ORIGIN      
        1 ccagcgtcag cgcgtcgcca tcgcccgcgc cctggtgaac aatccatcga tcatcctcgc
       61 cgacgagccc accggcaacc tcgactccaa aaccggcgta gagatcatgg ccctgttcga
      121 tgacctgcag cgaaagggca ataccatcgt ccttgtcacg cacgaggccg acatcgccga
      181 gtacgcacac cgcgtcgtcc acatccgcga cggcaagatc ttctccgacc agccgtcctc
      241 ccgcgctaca aaagcagccg ccgtctcggg ataactgaaa acgagagtca gtccactttc
      301 ccgggcgtat tctcccggcg cggcgccatt gcaacaggcg atccctcgcg tttccctcgc
      361 tggagcacgc ttgttcagaa tgcttcctct gtgcgcctgg ggtggtcttc cgggttcgcg
      421 ccacgcagga ccgtgctttt cgccccgcac agcccgcgaa taacaccctt cgaattacat
      481 atcggaagta atctggacac ttagcaaggt gcaaactatt ctgcgatcaa ctggcaggtt
      541 cgttccatgc atgttctcgc agcaaagcac ctgaaagacg cggccggccg ctttcccaaa
      601 gcggctaaac aactcgcagc ctggcgatca atcgccaaag ataagtgctg gcgcagtcct
      661 gacgacgtga aagatacttt cgccgatgcc gagttcgccg gagacttcgt catcttccgc
      721 attcaccaga acaaataccg gctggtcacg agcattcatt acccaggcgg cgtaaacggc
      781 agtgccacgg acggtcacgt ctgggtacgc tcgttcctca gtgacaagca atatgaagac
      841 caggctaact gggagaaagg agttctctga tgggcaaaac tctcgccgat cccgttgaga
      901 tgatccgccg aggcgccccc cacctcatcg gctccgaaga tgaactggcc ggctacacgc
      961 gcgctctctt caccctgacg tccaagccca ggcccactgt cgccgagcag gaggctatca
     1021 acctcctcac tctgctgatc gagcaatacg agacggtccg ctacgtcgtt cccaaaggca
     1081 gcccagtcga ggtgctgcgt cagctcctca aacggaacgg cctccgccaa agcaacgtcg
     1141 ccaccgaatt tggctgcgtg gcgaatgcct cgatggtcat gagaggcgaa cgtcagttca
     1201 cgcgcagtca catcgcccgg ctcagcaagc gcttcagggt ctcgcccgcg gtcttcttcg
     1261 acgaaaactc ggcgatcccc gaacgccgca atatgggaaa gagagccgcg cgagccaaag
     1321 ctcagtagac cgttccgccg gtgaagcggc gcattacaaa ctcaaccatg aggtttcctc
     1381 cgatgagcaa ggccctcccc gatcccgccg aaatggtgca gcacggcgcc ccgcggctca
     1441 ttcgctccga cgaagagctg accgagtact cccgcgtcct ctttgccctc acttcgatac
     1501 ccaagcccac ttccgccgaa caggaggcca tcggccttct gaccctcctc atcgagcact
     1561 acgaaaaccg ccgttatccg cttcctgaag cccgctccgt cgaagtcctc cgtctgctca
     1621 tggagcggaa cggcgtctcg gaaggaaaac ttacgcctga acttggattt caggcctcgg
     1681 cctccctcat ccttctgggt aaacggcagc tcaatcgtgg ccacatcgca cgcctcagcc
     1741 ggaggttcaa tgtctcgccg gccgtcttct ttgacgaggc ttctgcgatt cccctgagga
     1801 acggcgtcag gataaagaag cggcgctctc gttctgcgcg agcgaaagtc cgctagccgg
     1861 aatcgtcgac aacgaaaagg gcggccgtca ggccgccaat ctccacacgt ctcgctgtat
     1921 ctgaagcggc atccaggaac gggaaccgct cgcaacaggc catcaattct ctattgcgtg
     1981 caggctattc cctgccttat tgcggctcct tcgcgccgtt ctttgcgccc ttgaccgcac
     2041 ccttcgtcgt gttcttcgtt ttatgccagg ccttcttcgt gcccttcttc gatgcgtgat
     2101 aggcgctctt tgtccccttc ttcacgccat gaccaacgtc cttcgcgccg gccttggcat
     2161 cgtgtccggc gtccttcata tcctgcttcg cactgttatc gctctgcgct cgcgcgctca
     2221 catatccggg agccagcatc aacgctgccc ccaggaccgc cgccatccac cgcaatctcg
     2281 ttttcatacc aaagaggatg acgcacaacc cggtctgagt tgtgcgtcat tcagctgtgc
     2341 ggcaccaggt aacgccggca accagaacat ccgaactgcc ggccagtggc taaactaagc
     2401 cccttcttcc agcacgtttg gcacatcctc acccagcgcc gttgccagat cgtgctgatg
     2461 ctcctgttcc tgcgaaatga tctgccggat ctgctccgcc agcgcataat gaccgatggc
     2521 ttccgcctgc cgcacgcgct tccggtactg ccggatcgtc tccgtctcgt tgtccaggtc
     2581 gaaacgcagc atctcttcgg ccttctccga gagcttcacc ggtttcggcg tcgcattcgg
     2641 cacagcaccc agataatcga tctggtccgc gatggtcatc gcgtgagcca gctcctcgcc
     2701 ggcatgcttg cgcagttcgc cggcgatgct catccactga gcgcctttca gcacgttgct
     2761 gtagaccgta taggcgatga ttgcctgata ctcccgcgac aaatcctgct gcagaccgtc
     2821 gatcaactct ttcagcgcag cactgttctt cttctctgcc attcttctct cctcaatcct
     2881 gttgggatac cacagtttag atgctcctgg ccgtcaaaac ggcgtgaatt gggatcgcat
     2941 ttccccgcac actcccgagg gcccaaagca cgcgttggcc tggacggcaa atcgccctgt
     3001 accctgtacg ctataccctg taggaaaaat gcccgccctc caccacctct tcaacgacca
     3061 cgcggaacac gtccgcgcca aaattacggg catctacggc attcttctcc tctttaacgg
     3121 agccgcctgg ctctgggccg tcctggcgtt cgatcactat ccggtcctgc tcggcaccgc
     3181 cttcctcgcc tattctttcg gcctccgcca cgccgtcgat gccgaccaca tcgccgccat
     3241 cgacaacgtc acccgcaagc tcatgcagga aggcaaacgc cccgtcgccg tcggatttat
     3301 gttctccctc ggccactcca ccgtcgtcat tctcggatcg ctcggcctcg ccgcgctcgc
     3361 tctcggcctg caaaagcacc tcgaccacta ccgcaccatc ggcggcgtca tcggcaccag
     3421 cgtctcctcg ctctttcttc tcggcattgg cctggtcaac ctcatcatcc tgcgccacgt
     3481 ctatgtcacg ttcgctcgcg tccgccgcgg cgagccctat gtcgatgagg acttcgacct
     3541 cctcctcaac aatcgcggct ttctcgcccg catcttccgc cccgtcttcg ccatgattcg
     3601 ccgcagctgg cacatgtatc cgctgggatt cctcttcgga ctcggcttcg ataccgccag
     3661 cgaaatcggc ctcctcggac tctccgccgc cgaagcctcc cgcggccttt cgctctggtc
     3721 catcgctgtt ttccctgtac tgttcgccgc cggcatgtcc ctcatcgaca ccaccgacaa
     3781 catcctcatg ctcggggcgt acggctgggc ctatatcaag cccatccgca agctctacta
     3841 caacctcacc atcacatctg tatctgtcgt cgttgcattt gttgtgggag gcatcgaggc
     3901 cctcggcctc atcggcggcc acctccacct ccagggcgtc ttctggaact tcatcgaccg
     3961 cctcaactcc aacttcggag tcctcggcta ctgcatcatc ggtatcttcg ccctcgcctg
     4021 gatcgtctcc atcgccatct acaaactccg ccgcttcgaa gatctcgaac tcacccccga
     4081 aagctaaaca gccacggctc ggcatccgcc aatgatcgtc tttggactca actccagcga
     4141 cctcgaatcg attgcgccgg tcctggaggc agctctgaac gttcgactca tgccgcgcga
     4201 aggctatttt cacggcggca actactactc ggcggaaacc gcaggcggca cgattcgcct
     4261 tcaatacaat cgcgacatct tctccggaaa aattgggcca atcgtgcgcg actggccggt
     4321 agatcgcctc gttctctcat tcgaaggatc ggacgacaca agctgggaac tgtacctcag
     4381 cgctctgtct cagttgagcg atctccagcc gacccgactc gctccgcaat ctgccggcta
     4441 accgcggaaa gcgatccata caaaagcaaa gggccccgca gcacgcgaag ccctccactc
     4501 cctgctggct attggctgtt cctta
//
LOCUS       DASZYN010000575         2470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1054047, whole genome shotgun sequence.
ACCESSION   DASZYN010000575 DASZYN010000000
VERSION     DASZYN010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054047"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VHE33_14925"
     CDS             <1..892
                     /locus_tag="VHE33_14925"
                     /inference="COORDINATES: protein motif:HMM:NF037850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HVT98795.1"
                     /translation="ATDPIRNTVSSYDGVVPWGESPEEWWTFYGTREWTCGGFAWTGF
                     DYRGEPTPFGWPSINSQFGIVDMCGFPKDYFYYYKAWWRKEPSLHVFPHWNWHGREGE
                     EIPVWVYSNLEEVELLVNGASLGRQKVPRLGHVQWNAKYEPGAIEARGYQDGKVVLTE
                     KRETTGPAASIRMTADRTEIHADGEDIAVLKVEALDKEGRLVPIANNKIGFSVSGPAK
                     LIGVGNGDPNCLESDKAPRRSLFNGFAQVIIQSTKRAGAIHVVASKDGWEGPDLTSAT
                     LLITSRPAEARPVVPGVRDL"
     gene            927..1277
                     /locus_tag="VHE33_14930"
     CDS             927..1277
                     /locus_tag="VHE33_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98796.1"
                     /translation="MSTQGPSRIQTPMPLNTKPEDLPAAVRFSIEPKDGYIEVAGTAL
                     PQKEEWRYRLHDGAVRFADHDAFKAKVVYVGMSPAVAEDIGRSFNATARQLRELGFAV
                     ELPEQEANEKQQAA"
     gene            1341..>2470
                     /locus_tag="VHE33_14935"
     CDS             1341..>2470
                     /locus_tag="VHE33_14935"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVT98797.1"
                     /translation="MSVANVVRPHFLNEEGGVPDPAPASIDVSIDGRTVQGRAGEPLI
                     DVINRETDKELAQVCYLQQLGPIQTCDTCMVEVNGRLVRACGTRVEPGMRVETESYRA
                     QAAQREAFDRILGNHLLYCTVCDNNNQNCTVHNTTALLKVEHQSIPYRQKPYEKDETN
                     PFYRYDPDQCILCGRCVEACQNVQVNETLTINWEAEHPRVLWDGGSPIGESSCVSCGH
                     CITVCPCNALMEKSMLGEAGYFTGLPRQALEHMIDVVKGIEPEVGYGPILQLSKAEAD
                     MRMVRTQKTKTVCTYCGVGCTFDIWHRDRQILKVEPHEGPANGISTCVKGKFGFNYIN
                     SADRLTSPLIRDGDKFRRCTWDEALDFIAAKMKSIKARYGPDS"
BASE COUNT          536 a          752 c          720 g          462 t
ORIGIN      
        1 tgctaccgat ccgatccgca acaccgttag ctcctatgac ggcgtggtgc catggggcga
       61 gagccccgaa gagtggtgga ccttttacgg cacgcgcgaa tggacctgcg gcggctttgc
      121 ctggactggg ttcgactatc gcggcgagcc cactccattc ggctggccct ccatcaactc
      181 gcagttcggc attgtcgaca tgtgcggctt cccgaaggac tacttctact actacaaggc
      241 gtggtggcgg aaggagccct ctctgcacgt ctttccccac tggaactggc atgggagaga
      301 gggcgaagag attccggtct gggtctattc caacctcgaa gaagtggagt tgttggttaa
      361 tggcgcgagc cttggccgtc agaaggtccc gcgtctcgga cacgtgcagt ggaatgccaa
      421 atacgagccc ggcgcgatcg aggcgcgtgg ctatcaggat ggaaaagtcg ttctcaccga
      481 aaagcgggag acgacgggcc cggcggcttc gattcgcatg acggccgatc gaaccgaaat
      541 ccatgccgac ggcgaggaca tcgcagttct gaaagtagag gcactggaca aggaaggccg
      601 gctggtgccc attgcaaata acaaaattgg attcagcgtt tcaggaccgg cgaagctgat
      661 cggtgtgggg aatggtgatc ccaattgcct ggaaagcgac aaggcgccga ggcgctcgct
      721 cttcaacggc tttgcccagg tcatcatcca aagcacgaaa cgtgcgggcg caattcatgt
      781 tgttgcctcc aaagacggct gggagggccc ggacctgacc tcggcgacac tgttgatcac
      841 gtcaagacct gccgaagcaa ggcctgtcgt cccgggtgtg cgagacttgt gaagatggcc
      901 agcgcatccg ccactctgtt tgcgcgatga gcacgcaagg cccatcgcgc atccaaaccc
      961 ctatgccgct caataccaag cccgaagacc tgcccgctgc ggttcgcttt tcgatcgagc
     1021 ccaaagacgg ctacatcgag gtcgcaggca ccgcccttcc acagaaggag gagtggcgct
     1081 atcgccttca tgacggtgcg gtccgcttcg ccgaccacga tgccttcaaa gcaaaggtcg
     1141 tctacgtggg gatgagtcct gccgtggccg aagatatcgg gcgttctttc aacgcgaccg
     1201 cgcgccagct gcgcgaactg ggcttcgcgg tcgaactgcc agaacaggaa gcgaatgaga
     1261 agcagcaggc tgcctgaccg aagccgccgc tcccacactt cagcaaaccc ctgtgcattc
     1321 cggggcgcat ccaacacatt atgagcgtgg ccaacgtcgt tcgtccgcat tttctcaacg
     1381 aggaaggcgg cgttcccgat cctgcgcctg cctccatcga cgtcagcatc gacggccgca
     1441 ccgttcaggg tcgtgccgga gagcccctca tcgacgtcat caatcgtgag acggacaagg
     1501 aactggccca ggtctgctat ctgcagcagc ttggtccgat ccaaacctgc gacacctgta
     1561 tggtcgaagt gaacggccgg ctggtgcggg cctgtggcac gcgggtcgaa cccggcatgc
     1621 gcgtcgaaac cgagagctac cgcgcccagg ctgcacaacg cgaagccttc gaccgcatcc
     1681 tcggtaatca tcttctttac tgcaccgtct gcgataacaa caaccagaac tgcacggtcc
     1741 acaacacgac ggcgctgctc aaggtggagc accagtcgat tccgtaccgg caaaagccat
     1801 acgaaaaaga cgagacgaac cccttctatc gctacgatcc ggaccagtgc atcctttgcg
     1861 ggcgctgcgt tgaagcttgt caaaacgtcc aggtcaacga gaccctcacg atcaactggg
     1921 aggcagaaca tccgcgcgtc ctttgggacg ggggatctcc catcggggaa tccagctgcg
     1981 tctcctgcgg ccactgcatc accgtctgcc cgtgcaacgc gctgatggag aaatccatgc
     2041 tcggtgaagc cggttacttc acggggcttc cccggcaagc gctcgagcac atgatcgatg
     2101 tggtcaaggg catcgagcca gaagtcggct acggtccgat cctccagctc tccaaagccg
     2161 aagccgatat gcgcatggtt cgaacccaga agaccaagac cgtctgcacc tattgcggcg
     2221 tgggctgcac cttcgatatc tggcatcgcg accggcagat tctcaaagtc gaaccgcatg
     2281 agggacccgc gaacggcatt tcgacctgtg ttaaaggtaa attcggcttc aactacatca
     2341 actccgccga ccgcctgacg tctcctctca tacgcgatgg agataagttt cgtaggtgta
     2401 cgtgggatga ggccctcgat ttcatcgctg caaaaatgaa gtcgatcaag gctcgctatg
     2461 gtcccgattc
//
LOCUS       DASZYN010000576        16260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_283688, whole genome shotgun sequence.
ACCESSION   DASZYN010000576 DASZYN010000000
VERSION     DASZYN010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 16260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16260
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283688"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VHE33_14940"
     CDS             complement(<1..731)
                     /locus_tag="VHE33_14940"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HVT98798.1"
                     /translation="MFRKVLIANRGEIALRLLRACRELGLSTVAVYSDADRAAPHVLE
                     ADEAYRLGPAPAPESYLRGDLILEIARRTGAGAIHPGYGFLSENADFAQACEHAGIKF
                     IGPPPSAMRILGSKTNARQAADEAGMPRTPGCVHALASPEEAADVAAGVGYPVMLKAA
                     AGGGGKGMRAVFSPAELPSAFASASSEAERAFGSGEVYLEKLIERPRHIEIQIIADEH
                     GHCVSLGERECSVQRRHQKVIEEAPS"
     gene            complement(1286..2107)
                     /locus_tag="VHE33_14945"
     CDS             complement(1286..2107)
                     /locus_tag="VHE33_14945"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT98799.1"
                     /translation="MNRIARFLVLAAATALLVASSTGCKRLEARNQLNKGVEAYKQAK
                     YEEAIDHFQKAVNLDPTYPMTRLYLATAYAQQVVPGLDTPENEKTAQMAIDGFQEVLR
                     EHPDDITSLKQIAALKYDLKKFGEARDWQMKVLSADPKDAEAAYTIGVVDWTQAYQNA
                     IHALQSAGLQDDGQGNAKAPKAVCKTLQDENTQLVTDGLKYLQQAVSLRSNYDDAMQY
                     LNLMYRRKADLDCGDDAARKADLAQAQDWVQKAMGARKINEQKANEKASGGVVMQ"
     gene            complement(2251..2685)
                     /locus_tag="VHE33_14950"
     CDS             complement(2251..2685)
                     /locus_tag="VHE33_14950"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HVT98800.1"
                     /translation="MSMSVGGAGALNAEPNVTPLIDVLLVLLIIFMVIVPVTPRGLDA
                     LVPQPPKNPNQSQPNDRTIVIQVQSGGGGVPSYMINETPVAKNDLESKLNEIYSTRAE
                     KVMFVKGDPNLDFQAVASVIDIGHAVGVDHIGLVTPKMQAGQ"
     gene            complement(2724..3182)
                     /locus_tag="VHE33_14955"
     CDS             complement(2724..3182)
                     /locus_tag="VHE33_14955"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HVT98801.1"
                     /translation="MAIAVRNEGAKVNANINVTPMVDVMLVLLIIFMVITPMLQNKVN
                     VDLAQSTNATPMEDANKEDSVVVAITRDSRVYLGQNQTSVANLGSQVRDELENKTSKI
                     VYFRADARAHYGTVMDAIDAVRTAGVEEVGMLTEQQLPSTTTRPPTAGGQ"
     gene            complement(3318..4061)
                     /locus_tag="VHE33_14960"
     CDS             complement(3318..4061)
                     /locus_tag="VHE33_14960"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HVT98802.1"
                     /translation="MILAQIHHLVANPASLGFFQEGQTVGFSPIDLWNNMGWLARGVV
                     FVLFIMSIWSLAVMIDRYLYFNAARKQSREFAPRVAGALKEGKLDEAVKVADRNKKSH
                     LAEVVTAGLQEFRNYGGGGAVSVEQIESSKRALERAEAIVHAKLKRGLGSLATIGSTA
                     PFVGLFGTVAGILKAFQEIATQKTPGIGAVAGGISEALVTTAFGLFVAIPAVWLFNYF
                     TNRVEAFDVEMDNSSSELIDYFIKQSNRR"
     gene            complement(4200..4925)
                     /locus_tag="VHE33_14965"
     CDS             complement(4200..4925)
                     /locus_tag="VHE33_14965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HVT98803.1"
                     /translation="MAMFEDILLESGHQIKTKSKYYTIVGVILNGSVLAALILYPLIY
                     PEALPKAMMATLLVAPPPPPPPPPPPPPTIKVEHVVSEMLNNQLTAPTRIPHNISMVK
                     EDAAPPSAGVAGMEGMGGSAGVMGGVLGSLGTGPAPTVKAAPPKKITVSSGVMQGNRI
                     AGETPQYPAIAKAARIQGTVVLQATISKNGTIENLRVISGPPMLQSAATEAVRSWRYK
                     PYLLNGEPVEVETQVNVVFSLGG"
     gene            complement(5208..6413)
                     /gene="secF"
                     /locus_tag="VHE33_14970"
     CDS             complement(5208..6413)
                     /gene="secF"
                     /locus_tag="VHE33_14970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HVT98804.1"
                     /translation="MELFHDPKIDWLGKKWYFLGFSLIFSVAGLFSIFFWHHLPLDVD
                     FRGGTVVRVKFADKPDIDRIRSAANKAGLADPRITVYGPAADNEVLITLSLSTTSQSA
                     LDQGRATIVNALTQNYATTAAADQSKLDLDNVTGTALASWLQQNDPEHLGNAADAQTR
                     YQAQADAVLKYRDGQHSGIVDSIDNLRSLLDPAVFSALQQSAYLSGFHIQSAEIVGAQ
                     VGKVLQHQAELATLYSLGGMLVYLWFRFELIYGVAAVVACFHDTIITVGAFSLTNQEI
                     SLTVIAAILTLVGYSMNDTIVVFDRIRENLRLSRREPLADLVNRSINQTLSRTVLTSG
                     LTFLTVLSLFIFGGEVLRGFSFALVVGILIGTYSSIAVAAPMLVAYQDWRGGRGRAAT
                     LPAAKKARV"
     gene            complement(6450..8072)
                     /gene="secD"
                     /locus_tag="VHE33_14975"
     CDS             complement(6450..8072)
                     /gene="secD"
                     /locus_tag="VHE33_14975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HVT98805.1"
                     /translation="MKTNIRTRLIIIIAVLVVFVFGIFRVPHGLSGSALKQAILQNIH
                     LGLDLKGGMHLILQVKVDEAISASTDSDVAGIESDLQKNGIAGATVSKPDANRADFIQ
                     VANVPAAHNNDVRSILESSYSNRFDIAPGVGNSWTLTMKPTVESEAKQRALDQAIEVI
                     QSRTNTLGVSEPIIEPYQLGSYQILVELPGVSDLGRVMGIIQSTARLEAHEVMSDQTW
                     PDQQSALQALGGSLPPDAELVQSVPGASSAGSATSWFELKKVPVWGGTDIRDAQPGHN
                     PNTGEPEVDFYLTSAAGSKFSAWTGANIGKQMAIVLDNRVQEVAVIKGQIGDQGNISG
                     GGISEQRVKDLSMLLRTGALPASIDFLQETTVGPSLGADSIHQGVVASIVGMLAVMIF
                     MLVYYKGAGINADLALFLNLVILLGFLGFSGATLTLPGIAGVILTIGMGVDSNVLIFE
                     RIREELRAGKSPSAAVDQGFGHAWTTIVDTHVTTIVSAAILFIFGTGPVKGFAVTLTF
                     GLLANLFTAVFVSRVIFDAHLSRKLHGEPISI"
     gene            complement(8081..8395)
                     /gene="yajC"
                     /locus_tag="VHE33_14980"
     CDS             complement(8081..8395)
                     /gene="yajC"
                     /locus_tag="VHE33_14980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HVT98806.1"
                     /translation="MTFLAFLAPFALPTGVAGMLPFLLIIVVFYFLLIRPQQKKQRTW
                     QDMLGKLKTGDRVTTTGGMRGVIISIKDDAVQLRIPPDNLRIEVVKSAIASVTQDDAN
                     PK"
     gene            8740..9399
                     /locus_tag="VHE33_14985"
     CDS             8740..9399
                     /locus_tag="VHE33_14985"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF003296.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HVT98807.1"
                     /translation="MASAGLTAHAAVHPGRIEVITGPMFSGKSEELIRRLKRAQIAKR
                     RVACFKPDLDVRYHRNAIASHSAQTLDAVTVANVGELSAVLMPKIEAVEVVGIDEAQF
                     FDASLVSLAQELIRLGRRVILAGLDTTFTGEPFAPIPDLMAIADEVTKLSAVCMQCGA
                     TAVHTQRLGSSQDLVLVGAAGVYEARCRACFQPWVDEHATEQLELPVGARVEEPSATR
                     G"
     gene            complement(9408..9872)
                     /locus_tag="VHE33_14990"
     CDS             complement(9408..9872)
                     /locus_tag="VHE33_14990"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVT98808.1"
                     /translation="MLKPFLIASALAFLGVPALVQQEAPAPAAAPAATTNQFNVPPDY
                     LSKVNPVKPTAESQAYAKKQYGWDCAMCHGDNGNGKGDVAAEQKLTLRDYRDASALKS
                     MSDGEIYYIIEKGKGQMPSEGDRAKPDQIWNLVIYIRKMANPLATASAQTPQ"
     gene            10097..11353
                     /locus_tag="VHE33_14995"
     CDS             10097..11353
                     /locus_tag="VHE33_14995"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HVT98809.1"
                     /translation="MAGGSSPTITGVSLRFQIHRTSGAGRLATLTLPHGAVETPVFMP
                     VGTQATVKAVPQQLLEELDVRILLGNTYHLYLRPGHEAIRRLGGLHRFMSWERAILTD
                     SGGFQVFSLSELRKVTEEGVAFRSHIDGSSHFFSPEHSMDVQIALGSDIAMAFDECTE
                     YPADRARARASLDVTLRWAKRSREHFEQQKEAVPWAQPKRIAESGKRLESEREQRSAF
                     SVPCSESDSEERLKRFEGQTPSLFGIVQGGMFADLRREAAERLVEMDFPGYAIGGLSV
                     GEPRELTREMIATTLPLLPADKPRYVMGVGYPDEIAEYARMGVDMMDCVLPTRAARHG
                     LLFTSEGRLNIKNRQFAEDQGPPDPACGCVVCRRYSRAYLRHLMTAQEPLSAVLNTLH
                     NLAFYLETMRKVREEMRSQSVSHSDR"
     gene            complement(11358..12422)
                     /locus_tag="VHE33_15000"
     CDS             complement(11358..12422)
                     /locus_tag="VHE33_15000"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HVT98810.1"
                     /translation="MSPSRSSHLGVVVCGAGVFGRNHLRVYRALEDDGIPVRLAAVVE
                     PDIARAGTAATDWGAPVFSSVEECLAARARSEVEFAAASVCTPTSRHFEVSEALLAAG
                     IDLLVEKPIAATVDQATRLIDLANLNSRILQTGHLERFNPAVTATTPILNHPMFFEAH
                     RLSIFSPRALDVDVILDLMVHDLDIVLSLVQSPVSSLHAVGLPILSPKVDIANVRIEF
                     ESGCVANFTASRVSTEKVRKLRFFQPHQYVSIDYARQDLLVIDVSRAAHADPAAMAAA
                     VASAGSGGFPGLHIDKPAVKPGEPLRLEIESFLEHVRTRTRPLVSGEDGRAALALAID
                     INAAIAAHAERAGLARFVAS"
     gene            complement(12430..12648)
                     /locus_tag="VHE33_15005"
     CDS             complement(12430..12648)
                     /locus_tag="VHE33_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98811.1"
                     /translation="MDSVPPQGNGVRRNEDIALDLLKFVAATANVGRATTPSTGFVAA
                     GSTKPEDQVTHLLELYARCLRVVEGKPA"
     gene            complement(12703..13416)
                     /locus_tag="VHE33_15010"
     CDS             complement(12703..13416)
                     /locus_tag="VHE33_15010"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVT98812.1"
                     /translation="MNFLELHERLRLETWRRIDQGILSSSLLARRTGLAQAHISNFLH
                     RRRRLSLTALNRVLLAQELDVEDLSPINAQATVSAPQSPSASADLIPIVAHGAAMLSP
                     LIPPRSVQATLHLPTGWWTGLPVRRSVSRRSWERFVAVRITALQALPMDPVLRVGSVV
                     VLDRHYNSLATLRPPAPNLYGVREGNQLLFRHVMLDSSRLILRPRAMEYPVEVIELAP
                     QETPADLLVGRVCLCISEI"
     gene            14011..14517
                     /locus_tag="VHE33_15015"
     CDS             14011..14517
                     /locus_tag="VHE33_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98813.1"
                     /translation="MSAAPALAIVVPEARAVAETELPVYRPKPIEGFAFYRKHTLALL
                     RHYLDLSMKTNRASSVLDRVILRGRVSSYRLRTFEDGIIFVLDVEKCLQQLDRLSRKV
                     VFLIALQDYSVPETAWLTGESLRSAARIYGEAMNRLTAKFLQLGLLKVDVEKLSRGEA
                     EIQSNDIT"
     gene            14706..>16260
                     /locus_tag="VHE33_15020"
     CDS             14706..>16260
                     /locus_tag="VHE33_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98814.1"
                     /translation="MRNRDDEARNTLVRRVWRAALGMLAVAAAIFPPHMAAQIHMTTV
                     QGTVYRADGTPAAGTLLLSWPAFTTPQNQAVAAGNMSAAIGADGFVSVNLTPNSDALP
                     TGTYYTAVYHLNDGTVNQEYWVVPSAGNASIAAVRAQLQPSTVAVQPLSKTYLDSAIA
                     SLNGSWLPVAGGTMSGPLNLNGDPSAANQAATKHYADQLAAAQLPLSGGTVSGPLNAK
                     QIEGHLYADQWQSGAGKNDGIAMSLSECATYPYACQVLAPSTYALNETPPWGGYLQWI
                     AGRTIYTGPPASVPNGTCLVDDRLGAPQVMCVPKVPPYWAQGATGWTEVGPSFTVMQT
                     NHPTDAMTGWTAPGLAVNKANYFGTRNFSAGIPNGSGQTNNTGLQVVTTTFAPATTNS
                     VANYLLANSNGDHIGLYDWDVSRGGISAGDNEGNEERFAHMEAPDVWQGRVNSITTIA
                     SNDTGHTTSLCTAPCTVVSATQTQGSIGTLGDELPVVDITRGYSTGYITSIAGNVVTA
                     SSNANWDAQY"
BASE COUNT         3256 a         4157 c         5739 g         3108 t
ORIGIN      
        1 gatggcgctt cttcaatgac cttctgatgc cggcgctgga cggagcactc gcgctcgcca
       61 agggagacgc agtgcccgtg ctcgtcggca atgatctgaa tctcgatgtg gcgaggccgc
      121 tcgatgagtt tctccagata gacctcgccg gaaccgaagg cgcgctcggc ctcgctgctg
      181 gcggaggcga aggcagaggg gagttccgca ggcgagaaaa cggcgcgcat gcctttgccg
      241 ccgccgccgg cagcggcctt gagcatgaca ggatagccga cgcctgcggc gacgtcggca
      301 gcttcttcgg gtgaagcgag tgcgtggacg cagccgggtg tgcgcggcat gccggcttcg
      361 tcagcggcct ggcgggcgtt ggtttttgag ccgaggatgc gcatagccga cggaggtggg
      421 ccgatgaact tgatccctgc atgttcacag gcctgagcga agtcggcgtt ctctgagagg
      481 aaaccatagc cggggtggat ggcgccggct ccggtgcgac gagcgatttc gaggataagg
      541 tcgccacgaa ggtaggattc gggggctgga gcgggcccga gccggtaggc ttcatccgct
      601 tcgagaacgt ggggtgcggc gcggtcagcg tcggaataga cagcgacggt agacaggccg
      661 agctcgcggc aggcgcgcag gaggcgcaga gcgatttcgc cacgattggc gatgagaact
      721 ttgcgaaaca ccggatacaa gggtacaggg tgcagggagg tgggtacagg aaaaacgcga
      781 cagttgagtc atggctggca gtttggattg cgggacgaac tcatggggtc tgagtcgagg
      841 tcggaggatg ggatttgcgg tggttcctga aggtgttcgc ccggggtgtt gctaaatggg
      901 attgggacgg tactttcgac ttcgacaggg ttggagattt gttgaatttg ggatcgaaga
      961 ggtatgagct tcttgctgtg gggtgaacct ggctttcccc tggggagtgc aggaggggcg
     1021 aaatttggca acattcgttt gcgactgaaa aataccagcc gtcgtttgcg actgaaaatg
     1081 cgtttcccct gaaagggtcg ccaagagcaa tccatcccaa agcgcgagac gatgcccgtc
     1141 gctcagatcg tgagcgaagc cttcgcgctt acatcgcggg aaacccctcg cttcccgcgc
     1201 aacaaaaagc ccctcgcttg caccgcgaaa cacaaagccc tcgctttggg gcgagggctt
     1261 tgtgcggttc gcggacggtg gaggtttact gcatgaccac gccaccggag gctttctcgt
     1321 tggctttttg ttcgttgatt ttgcgggctc ccatggcttt ctgcacccag tcctgcgcct
     1381 gggcaagatc ggccttgcgg gccgcgtcgt cgccgcaatc aaggtcggct ttgcggcggt
     1441 acatcaggtt gaggtattgc atggcatcgt catagttgga gcgaaggctg acggcctgct
     1501 gcaggtactt gaggccgtcg gtgacgagct gcgtattctc atcctgaagg gtcttacaga
     1561 cagctttcgg cgctttggca tttccctgcc cgtcatcctg cagtccggcg ctctgaaggg
     1621 cgtggatggc gttctggtag gcctgagtcc agtcgacaac gccgatggtg taggccgctt
     1681 cagcgtcttt gggatcggcg gaaaggacct tcatctgcca gtcgcgtgcc tcgccaaact
     1741 tcttcagatc gtatttaagt gcggcaatct gcttaaggct ggtgatgtcg tcaggatgtt
     1801 cacggaggac ttcctgaaat ccatcaatag ccatttgggc ggttttttcg ttttcaggag
     1861 tatcaaggcc gggcacaacc tgctgcgcgt aagcggtggc gaggtagagc cgggtcatgg
     1921 ggtaggtggg atcgaggttg acggccttct ggaagtggtc gatagcctct tcgtatttgg
     1981 cctgcttgta ggcttcgacg cccttattga gctgattgcg ggcctccagg cgtttacagc
     2041 cggtcgaaga agccaccaga agggctgtgg ctgcggccag cacaaggaaa cgggcgattc
     2101 ggttcattct gtctgtcttc tccttcagct cgccgggagc ctaaaagcga cgcactcagg
     2161 cattgccgag agcgattgta ctgcttctgc ggccgggagc aagggtaccg gagtgcgacc
     2221 ggttgtagcc ctgctgcgag gcctgagaag ttactggccc gcctgcattt taggcgtcac
     2281 gaggccgata tgatcgacgc ccacggcgtg cccgatatcg atgacgctgg cgacggcctg
     2341 gaagtccagg ttgggatcgc ccttgacgaa catcaccttt tcggcacggg tggagtaaat
     2401 ctcgttgagc ttgctttcga ggtcgttttt ggcgaccgga gtttcattga tcatgtagct
     2461 gggaacgcca ccgccgccgg actgaacctg gatgacgatg gtgcggtcat tcggctgcga
     2521 ctgattgggg ttcttgggag gctggggcac cagcgcgtcg aggccgcggg gggtgacggg
     2581 cacaatgacc ataaagatga tcaggagcac cagcagcacg tcgatcagag gagttacgtt
     2641 gggctcggca tttaatgcgc cagcgccgcc tacgctcatt gacatagttc agttcctcgt
     2701 ttcctgactc atatacagct ggttcactga ccgcctgccg tgggcgggcg ggttgtggtg
     2761 ctgggcagct gctgttcggt aagcatgccg acttcttcta caccggcagt acggaccgcg
     2821 tcgatggcat ccatcacggt gccatagtgg gcgcgggcgt cggcgcggaa atagacgatc
     2881 ttgctggtct tgttctccag ctcatcgcgg acctgagatc caagattggc aacgctggtc
     2941 tggttctggc cgaggtagac ccggctgtcg cgggtaatgg cgacgacgac ggaatcttcc
     3001 ttgttggcgt cttccatcgg cgtggcattg gtgctctgag ccagatcgac gttcaccttg
     3061 ttctggagca tgggggtgat gaccatgaag atgatcagca gcaccagcat aacgtcgacc
     3121 ataggggtga cgttgatgtt ggcgttgacc ttggctcctt cgtttcttac tgcaattgcc
     3181 attggcgtac gctccgatcg gaattctcgc gcggcgggaa tcaggggccg ggagcacgga
     3241 ggctcccggc tgaaaaagat tctcgctcac ctggacttca ccctgggcgc cacggggcac
     3301 gcagggcagc tggacggcta ccggcggttg ctctgcttga tgaagtagtc gatcagctcg
     3361 ctggaggagt tgtccatttc gacgtcgaag gcctccacgc gattggtgaa gtagttgaag
     3421 agccagaccg cggggatggc cacgaagagg ccgaaggcgg tggtgaccag ggcttcagaa
     3481 ataccgccgg cgacggcgcc gatacctggc gtcttctggg tggcgatttc ctggaaggcc
     3541 ttgaggatgc cggcgacggt tccgaacagg cccacgaagg gagcggtgga accgatcgtg
     3601 gccagggagc cgagacctct cttcagcttg gcgtgaacga tcgcttcggc ccgttcgaga
     3661 gcccgcttgg acgactcgat ctgctcgacc gagaccgcgc cgccgccacc gtagttgcgg
     3721 aattcctgca gacccgcggt gacaacctca gcgaggtggg acttcttgtt gcgatcggca
     3781 accttgaccg cctcgtcgag tttcccttct ttcagggcgc ctgcgacgcg gggcgcgaat
     3841 tcacgcgact gcttgcgggc ggcattgaag tagaggtagc ggtcgatcat gacggcgagg
     3901 gaccagatgg acatgatgaa gagcacgaac acgacgccac gggcgagcca gcccatgttg
     3961 ttccaaaggt cgatcggact gaatccgacg gtctggcctt cctgaaagaa gccgagggac
     4021 gccgggttgg caacgagatg gtggatctga gcgagaatca ctgaatcttt cctcctgagg
     4081 caatgactgc gataggtgcc aaagatcgct gacggaatac ctgcccgcag gacgcgaggc
     4141 gggcaggccg gaaaatcgag agaagccccg gatcggcgag gccggaccgg gactgtaaat
     4201 cagccgccga gactgaagac gacattgacc tgcgtttcga cctccacggg ttcgccattg
     4261 aggaggtaag gcttatagcg ccagctgcgg acagcctcgg tagcggcgga ctgcagcata
     4321 ggcgggccac tgatgacccg caggttttcg atcgttccat tcttggaaat cgttgcctgc
     4381 aggacgacag ttccctgaat tcttgcggcc ttggcgatgg ccgggtactg cggcgtctcg
     4441 ccggcgatgc ggttgccctg catgacgcca gaggagacgg tgatcttctt gggaggtgcg
     4501 gccttgacgg tgggagccgg gccggtaccg agactgccga gcacgccacc catgacaccg
     4561 gcggagccgc ccataccctc catgccggca acaccggcgg agggaggggc cgcgtcttcc
     4621 ttcaccatgc tgatgttgtg gggaatcctg gtgggagcag tgagctggtt gttgagcatt
     4681 tcggagacga catgctccac cttaatggtc ggtggcggcg ggggcggcgg cggcgggggc
     4741 ggcggcggcg cgacgagaag agttgccatc atggcttttg gcagcgcttc agggtagatg
     4801 aggggataca ggatgagggc agcaaggacg gaaccattga ggatgacgcc gacgatggtg
     4861 tagtacttgc tcttggtctt gatctgatgc cctgattcca gcaggatgtc ttcaaacatt
     4921 gccatggccg acctcggacc tttcagtagt tgacacctcg tatagacacc gcaggggggc
     4981 cgaattgttc ccaactcgtt tcggcgattg cagaccgaac cccagccggg ggtttcacgt
     5041 ggatgtacag cctgccgcta tgccgggagc gggggtccaa ccgacagaca gggccggttc
     5101 tcttctataa cacaattccg agacaggtgc agcggcaccc actcgggagg cgcgggcctg
     5161 cattttttta ggatgcagat tttcgcgacc cgcgggggac ggaagcatca taccctggct
     5221 ttctttgcag cgggtagggt ggcggctctg ccgcggccgc cacgccaatc ctggtacgcg
     5281 acgagcatgg gggcggcgac ggcgatcgag gaataggtac cgatcaggat gccgacgacc
     5341 agggcaaagg aaaagccgcg gaggacctcg ccaccgaaaa tgaagagcga aagcacggtg
     5401 aggaaggtga ggccggaggt cagtacggtc cggctcaggg tctgattgat gctgcggttg
     5461 acgagatcgg cgaggggctc gcggcggctg aggcggaggt tctcgcgaat gcgatcgaag
     5521 acgacgatgg tgtcattcat cgagtagccg accagggtga ggatggcggc gatgacggta
     5581 agagagatct cctggttggt gagactgaag gcacctacgg tgatgatggt gtcgtggaag
     5641 caggcgacga cagcggcgac gccgtagatc agctcgaagc ggaaccagag gtaaacgagc
     5701 atgccgccga gggagtaaag agtggcgagc tcagcctggt gctggagaac cttgccgacc
     5761 tgggcgccga cgatttcggc actttgaata tggaagccgg agagatacgc gctctgctgg
     5821 agtgcgctga aaacagccgg atcgagcagg ctgcgcaggt tgtcgatgga atcgacgatg
     5881 ccgctgtgct ggccgtcgcg gtacttcagg acggcgtcgg cctgagcctg atagcgcgtt
     5941 tgcgcatcgg ccgcattgcc gaggtgttca ggatcgttct gctggagcca cgaggcgaga
     6001 gcggtgccgg tgacattgtc gagatcgagt ttgctctggt ctgcggctgc agtcgtggcg
     6061 tagttctggg tcagggcatt cacgatggtg gcgcggccct gatcgagagc ggactggctg
     6121 gtggtagaca gagacagcgt gatgaggact tcgttgtcgg ccgcgggacc gtagacggtg
     6181 atgcgcggat cggcgaggcc ggctttgttg gcagccgagc ggatgcgatc gatgtcgggc
     6241 ttgtcagcga atttgacgcg gacgacggtg ccgccgcgaa agtcgacgtc gagcggcagg
     6301 tggtgccaaa agaagatgga gaacaagccg gcgacgctga agatgagaga gaagccgagg
     6361 aaataccact tcttccccag ccagtcgatt ttcggatcat gaaacagttc cacgaaattc
     6421 cgcctataag aaagtctgtc gaattcgaat taaatcgata tcggttcgcc gtgcagtttg
     6481 cggctgaggt gggcgtcgaa gatgacgcgt gagacaaaga ccgccgtaaa cagattggcg
     6541 agcagaccga aggtaagagt cacggcaaag cccttgacgg gaccggtgcc gaagatgaag
     6601 aggatggccg ccgagacgat ggtggtgacg tgggtatcga cgatggtggt ccaggcgtgg
     6661 ccaaagccct ggtcgaccgc ggccgaaggg gatttgccgg cacgcagctc ttcacggatg
     6721 cgctcgaaaa tgaggacgtt ggagtcgaca cccatgccga tggtgagaat gacgccggcg
     6781 atgccgggca gggtgagggt ggcaccggag aagcccagga atccgagcag gataacgagg
     6841 ttgaggaaga gcgcgaggtc ggcattgatg ccggcgcctt tgtagtagac gagcatgaag
     6901 atcatgacgg cgagcatgcc gacgatcgaa gcgacgacgc cctggtggat ggagtcagca
     6961 ccgagggagg ggccaacggt ggtttcctgg aggaaatcga tggaagcggg gagagcaccg
     7021 gtgcgaagga gcatggagag gtccttcacg cgctgctcgc tgattccgcc gccggagatg
     7081 ttgccctgat cgccgatctg gcccttgatg acggcgactt cctggacgcg gttatcgagg
     7141 acgattgcca tctgcttgcc gatgttggcg ccagtccatg cggagaattt tgagcctgcg
     7201 gcgctggtga ggtagaagtc gacttcgggc tcgccggtgt tgggattgtg gccgggctga
     7261 gcatcgcgga tatcggttcc accccacacg gggaccttct tcaattcaaa ccagctggtt
     7321 gcactgcctg cgctggaggc accgggcacg ctctgcacca actcggcgtc cgggggcaaa
     7381 gaaccaccga gcgcctggag ggccgattgc tgatcaggcc aggtctgatc gctcatgact
     7441 tcatgggcct caaggcgagc ggtggactga atgatgccca tgacgcggcc gagatcgctg
     7501 acgcctggca gttcgacgag gatctggtag ctgccgagct ggtagggctc aatgatgggc
     7561 tcgctgacgc cgagagtgtt ggtacgggac tgaatgacct cgatggcctg atcgagagcg
     7621 cgctgtttgg cttccgactc gacggtaggc ttcatggtga gcgtccagct attgcccacg
     7681 cccggcgcga tgtcgaagcg gttgctgtag ctcgattcga ggatggagcg gacgtcgttg
     7741 ttgtgcgcgg cagggacgtt ggcgacctgg atgaagtcag cacgattcgc atcgggcttg
     7801 gaaaccgtgg cgccggcaat gccattcttc tgcaggtcgc tctcgatgcc ggcaacgtcg
     7861 ctgtcggtgg acgcgctgat ggcctcatcg accttgacct ggaggatgag gtgcatgccg
     7921 cctttgaggt cgagaccgag atggatgttc tgcaggatgg cttgtttcag ggccgagccg
     7981 gaaaggccgt gcggcacgcg gaagatgcca aagacgaaga ccaccaggac agcaatgatg
     8041 atgatgagtc gggtacggat gttagttttc atcgggctct ctactttgga ttggcgtcgt
     8101 cctgggtcac ggaggcgatg gcgctcttga ccacttcaat gcggagattg tcagggggga
     8161 tgcggagctg gacagcatcg tctttgatgg agatgatgac gccgcgcatg ccgccggtgg
     8221 tggtgacacg gtcgccggtt ttgagcttgc cgagcatgtc ctgccaggta cgctgcttct
     8281 tctgctgagg acggatgagc aggaagtaga agacgacgat gatgagcagg aagggcagca
     8341 tccctgcaac accggtgggc agggcaaagg gcgccagaaa cgcaaggaag gtcaacgcac
     8401 agtccttctg gccggacgga aaaagccgtc agcggcgcga gaaaaagtca atttccacgg
     8461 aagggctcgc aggatccgag gtcacggccg cgtcctggag ctggatacct ggaaaaggcc
     8521 ccggcaagag cacacacact tcctctctgc tggagagcct gactcataga atcgcgtagg
     8581 gaagagggtg tgtcaaggaa aaggctggcc gggacgcaca cgtggagcat gattcgggag
     8641 aagttcggcg aggtgaattt gggcccagct ttccacacgg ggagacttaa gcggcagcct
     8701 aaactggacc atcgggcaac agaaacaagg ggggaaccga tggcgagcgc ggggctaact
     8761 gcgcatgcag ccgtacatcc ggggcgcatc gaggtgatca cggggccgat gttttcgggg
     8821 aaatcggagg agctgatccg gcggctcaag cgggcgcaga tcgcgaaacg gcgtgtggca
     8881 tgcttcaagc cggatctgga cgtgcgctac caccgcaacg caatcgcatc gcacagcgcg
     8941 cagacgctgg atgcggtgac ggtggcgaat gttggagagc tgagcgcggt gctgatgccg
     9001 aagatcgaag ccgtagaggt ggtgggcatc gatgaggctc agtttttcga cgcatctttg
     9061 gtgagtctgg cgcaggaact gattcggttg gggcggcggg tgattcttgc ggggctggat
     9121 acgacgttca cgggagagcc ctttgcgccg attccggatt tgatggccat tgccgatgag
     9181 gtcacgaagc tctcggcggt gtgtatgcaa tgtggagcga cggcggtgca cacccagcgg
     9241 ctggggagca gtcaggatct ggtgctggta ggagcggcgg gggtgtacga ggcccggtgc
     9301 cgggcgtgtt ttcagccctg ggtggacgag catgctacgg agcagctcga gctgccggtg
     9361 ggcgcccgag tggaggagcc gagcgccacg agaggctgag ggacgatcta ctgcggggtc
     9421 tgcgcggagg ccgtggccaa cgggttcgcc atcttccgga tgtagatgac caggttccag
     9481 atctggtcag gtttggcgcg atcgccttcg gagggcatct gacctttgcc cttctcgatg
     9541 atgtagtaaa tttcgccgtc gctcatcgat ttgagtgcgg aggcatcgcg atagtcacgc
     9601 agggttaact tttgttcggc ggcaacgtcg cccttcccgt taccgttatc gccatggcac
     9661 atggcgcaat cccagccgta ctgctttttg gcataggcct gggattcggc agtgggcttc
     9721 acgggattga ccttgctgag gtaatccggg ggtacgttga actggttggt agttgcagcc
     9781 ggtgcggcgg caggagcagg agcttcctgc tgaacgagcg ccggcacacc gagaaacgca
     9841 agagccgagg caatcagaaa gggcttcaac atggcagcct cctgaagatc taagggaagt
     9901 cccggcataa ctttacgccc gctggtccaa aacggaacag gttttttgcg ggcgctcggg
     9961 tattaacgta atcggcaatg acgtgtcttt tggacgccag aggtccgaaa gcgaggatcc
    10021 acgcggaaac cgcgggtgca cttaatccat tgaaaatcag gtgggatagc ggtcaggcga
    10081 cgtgggacga tccccgatgg caggcggctc ctcgcctaca atcaccggag tgtcacttcg
    10141 ctttcagatt cacagaacct caggggccgg gcgacttgcc acgctgacct tgccgcatgg
    10201 cgcggtcgag acgcctgttt ttatgccggt agggacgcag gcaacggtaa aggctgtgcc
    10261 gcagcagctt ctggaagagc tggacgtgcg aattctgctg gggaacacct atcaccttta
    10321 tctgcggccg gggcatgagg cgatccggcg gctgggcggg ttgcaccggt tcatgagctg
    10381 ggagcgggcg atcctgacgg attcgggtgg atttcaggtg ttcagcctga gcgaactgcg
    10441 caaggtgaca gaagaagggg tggcgttccg gtcgcacatc gacggatcgt cccatttctt
    10501 ctcgccagaa cattcgatgg atgtgcagat cgcgctggga tcggacatcg cgatggcatt
    10561 cgacgagtgc acggagtatc cagcggaccg ggcgcgagcg cgggcgtcgc tggacgtgac
    10621 gctgcgctgg gcgaaacgga gccgggagca ctttgagcag cagaaggagg ctgtgccgtg
    10681 ggcgcagcct aagcggatag cggaaagcgg aaagcgttta gaaagcgaac gggaacagcg
    10741 ttcggcgttc agcgttccgt gttcagaaag cgattctgag gaacggttga agcgcttcga
    10801 agggcaaacg cccagcctgt tcggaattgt gcagggcgga atgtttgcgg atctgcggcg
    10861 cgaggcggcg gagcggctgg tggagatgga ttttccgggg tatgcgattg gcgggctgag
    10921 tgtaggcgag cctcgggaac tgacacggga gatgattgca acgacgctgc cgttgttgcc
    10981 tgcggacaag ccgcgctacg tgatgggagt gggatatccg gacgagattg ccgagtatgc
    11041 acgcatgggc gtggacatga tggactgcgt gctgccgacg cgagcggcgc gacacgggct
    11101 gctgtttacg tcggaggggc ggctcaatat caagaatcgg caatttgccg aggatcaggg
    11161 gccgccggat ccggcgtgcg ggtgcgttgt gtgccggcgg tacagccggg cttatctgcg
    11221 gcatctgatg acggcgcagg agccgttgtc ggcagtgctg aacacgctgc acaatctggc
    11281 gttctatctg gagacgatga ggaaagtgcg ggaagagatg aggagccagt ccgtcagcca
    11341 ctcagaccgt tagcgagtca gctggccacg aagcgcgcca ggccggcgcg ttcggcgtgg
    11401 gcggcgatgg cggcgttgat gtcgattgcg agtgccagag cggcacggcc atcttcaccg
    11461 gagaccagcg gacgggtacg ggtgcgaacg tgctcgagga acgattcgat ctcaaggcgg
    11521 agcggttcgc cgggttttac ggcgggcttg tcgatgtgga ggccgggaaa gcctcccgat
    11581 cctgcggacg cgaccgcggc agccatcgca gcgggatcgg cgtgggcggc gcgagagacg
    11641 tcgatgacga gcaggtcctg gcgggcgtaa tcaatggaga cgtactgatg cggctgaaag
    11701 aagcggagct tgcggacctt ttccgtgctg acgcgactgg cggtgaaatt ggcgacgcag
    11761 ccggattcga attcgatgcg gacgttggcg atgtcgactt tgggggagag gatgggcagg
    11821 ccgacggcgt ggagagagga gaccggcgac tggacgaggc tgagaacgat gtcgaggtcg
    11881 tggaccatga ggtcgaggat gacgtcgacg tcgagagcac gcgggctgaa gatgctgagc
    11941 cggtgcgctt caaagaacat ggggtgattc aggatgggcg tggtggcggt caccgccggg
    12001 ttgaatcgtt cgagatggcc ggtctgaagg atacggctat ttaagttggc gagatcgatg
    12061 aggcgcgtgg cctgatcgac ggtagcggcg atgggcttct cgaccagcaa atcgattcca
    12121 gcggcgagca gcgcttcgct tacctcgaaa tggcgcgagg tgggcgtgca aacggaggcg
    12181 gcggcgaact cgacttcgct tctggcacgt gcggcgaggc attcttctac ggaagagaag
    12241 acgggggcgc cccagtcggt ggcggcagtg ccggcgcggg cgatgtcggg ctctacgacg
    12301 gcggcaaggc gtacgggaat gccatcgtcc tcaagagcgc gatagacgcg caggtgatta
    12361 cggccaaaga caccggcccc gcaaacgaca acgccgagat gagaggagcg ggagggggac
    12421 accggaggcc tacgcgggct ttccttcaac gactcgcagg cagcgggcgt acagctcgag
    12481 caggtgagtg acctgatctt caggtttggt ggacccggcg gcaacgaagc cggtggatgg
    12541 ggtggtggct cggccgacat tggcggtagc ggcgacgaac ttgagcaggt cgagcgcgat
    12601 gtcctcgttg cggcgaacac cattgccctg gggaggaact gaatccattg gaacacctct
    12661 gagggcgatg ctatcaaagc gcgggacgcg ggaaaacgca catcagattt cggaaataca
    12721 gaggcacacg cgtccgacga ggagatcggc aggagtttcc tggggggcaa gctcgatgac
    12781 ctcgacggga tattccatag cgcgggggcg gagtatcagc cgcgaagagt cgagcataac
    12841 gtggcggaag agcagctggt tcccttctcg aacgccgtag aggttggggg cgggaggacg
    12901 gagagtcgcg agggagttgt agtggcggtc cagcacgaca acggatccga cgcggaggac
    12961 agggtccatg gggagggcct ggagagcagt gatgcgaacg gcgacaaaac gttcccagct
    13021 tcggcggctg acggagcggc ggacgggcag tccggtccac catcccgtcg gcaggtggag
    13081 ggtggcctgg acggatctgg gtggaatcag gggcgagagc atggcggcac cgtgggcgac
    13141 gatggggatg aggtcagcgg acgcggacgg cgattgaggc gcagatacag ttgcctgcgc
    13201 attgatcggg gagaggtctt cgacgtcgag ttcctgggcg aggaggacgc ggttgagcgc
    13261 ggtaaggctg agacggcggc ggcggtgaag gaagttggag atatgtgcct gggcaagccc
    13321 ggtgcgtcga gccagcagtg agctgctgag gattccctgg tcgatgcgac gccaggtttc
    13381 gagcctgagg cgctcgtgta actcgaggaa attcattctt ccgatgtttg cagggagcta
    13441 tacagccatt acggcggagt ttgcagaaag aatcgcaatt tcgcggaaac cgcgagaggc
    13501 ggcaacaaca ggagtaccca gctagtgcag aacgctcagt tggctccccc cggtaattga
    13561 ttgcattgct ccttgaaagt agtgtcggac tactcgtgcc ggtcagacat agtactttcg
    13621 ctcgcgggga gtggtgggcg aggtaactaa atcgcggcaa attcccaaat ccggaggatt
    13681 ccgacccatc tcgaagtggg ttcacgacga ttaagtggat gccgaggctg ccggaagagc
    13741 agcgaggctt cttcctcccc aactccgcgg agcggaaagc acatggtttc gcggctggct
    13801 gaactctgga gaatgactga tgtacctgcg aatttttctg aaagtgtgtg agagctgcgg
    13861 cgtgttatgg ttgcgcgccg agggatgccc ggaggtctac tgctccgcgt gcagtcgaaa
    13921 actgcggtca caaccggcgc tgcggcctgt tcggcggccg ggaagaaggg gcaggaggag
    13981 cggctgcgct atgagtaaag gaggcaaggc atgagcgcgg cgcctgcgct ggccattgtg
    14041 gttccggaag cgagagcggt cgcggagacg gaactgccgg tttaccggcc caagcctatt
    14101 gagggttttg ctttctaccg taagcacacg cttgcgctgc tgcggcatta tcttgacctg
    14161 tcgatgaaga cgaaccgagc atcgtccgtg ctggatcgag ttatcctgcg cgggagagta
    14221 tcgagttatc ggctgagaac ttttgaggac gggatcattt tcgttctcga tgtcgagaag
    14281 tgcctgcagc aactcgatcg cttatccaga aaggttgtgt ttctgattgc gctgcaggat
    14341 tacagcgtgc cggaaaccgc ctggctgacg ggagagagct tgcgatcggc ggctcggatt
    14401 tatggagaag cgatgaatcg tctgacggca aaatttcttc aattggggct gctgaaggtg
    14461 gatgtggaaa aattgtcaag aggcgaggcc gaaatccaaa gtaatgacat cacataagaa
    14521 aggacttgca aacgaaaacg ggcgaaaaag tttggcacga ttattctttc gaactgatat
    14581 tctgaactta gagtagaaaa agaaaaaagg gacacctatc cggtgtccct tttttattgg
    14641 tcccgacagg tgagagtctg agcagaccct gcatggggat gatgcgggtt tcagaaacga
    14701 ggaatatgcg gaacagagat gacgaagcgc ggaacacgtt ggtccggcgg gtttggcgag
    14761 cggccctggg aatgctggcg gttgcagcgg cgatctttcc gccgcacatg gccgcacaga
    14821 tccatatgac gacggtgcag ggcacggtgt accgcgccga tggaacgccg gcggcgggaa
    14881 cgctgctgtt gagctggccc gcattcacga ctccgcagaa tcaggctgta gcggcgggaa
    14941 acatgagcgc ggcgatcgga gcggacggat ttgtcagcgt gaacctgacg cccaactccg
    15001 atgcgctgcc gacaggcacc tactacacgg cggtctacca cctgaacgat ggaacagtga
    15061 accaggaata ctgggtggtt ccgtcggcgg ggaatgcttc gattgcagcg gtgcgggccc
    15121 agctgcagcc ctcgacggtt gcggtgcagc cactttcgaa aacgtatctg gacagcgcga
    15181 tcgcgtcgct gaatggctcg tggctgccgg tggcaggtgg gacgatgagc ggacctctga
    15241 atctcaacgg agatccatcg gcggccaacc aggcggcgac gaagcactat gcggatcagc
    15301 tggctgcggc ccagctgcca ctttccgggg gcacagtgtc tggaccgctg aacgcgaagc
    15361 agattgaggg acatctctac gccgaccagt ggcaatcggg agctggcaag aatgatggga
    15421 tcgctatgtc cctgtcggag tgtgcgactt atccgtatgc ctgccaggtt ctggctccgt
    15481 cgacttatgc gctgaatgaa acgccgccgt ggggtggata tcttcagtgg atcgcggggc
    15541 ggacgattta tacggggccc cccgcctcgg taccgaacgg cacctgcctg gtcgatgacc
    15601 ggctgggggc gccccaggtg atgtgtgttc cgaaggttcc accgtattgg gcacaagggg
    15661 ccacagggtg gacggaggtg ggcccttctt ttacggtgat gcagacgaat caccccacgg
    15721 atgcgatgac cggctggacc gctcctggcc tggcggtgaa caaggcgaac tatttcggaa
    15781 cgagaaactt ctctgccgga atcccgaacg gctcgggtca gacgaacaac acggggctgc
    15841 aggtggtgac gacgacgttc gcaccggcga ctacgaactc ggtggcgaat tacctgctgg
    15901 cgaattcgaa tggcgaccat attggcctgt acgactggga cgtgtcgcgc gggggaatct
    15961 cggcggggga taacgagggc aacgaagagc gctttgcgca catggaggca ccggatgtgt
    16021 ggcagggccg ggtgaactcg atcacgacga tcgcgagcaa cgataccggc catacaactt
    16081 cgctttgcac ggcgccgtgc acagtggtga gtgcaacgca gacgcaagga tcgatcggca
    16141 ctttgggcga tgaactgccg gtcgtcgata tcactcgcgg atactcgacc ggatacatca
    16201 cgagcatcgc cggcaatgtt gtgacggcat cgtccaacgc gaactgggat gcgcagtacg
//
LOCUS       DASZYN010000577         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_159014, whole genome shotgun sequence.
ACCESSION   DASZYN010000577 DASZYN010000000
VERSION     DASZYN010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159014"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            369..1841
                     /locus_tag="VHE33_15025"
     CDS             369..1841
                     /locus_tag="VHE33_15025"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HVT98815.1"
                     /translation="MKRAPFPLLVLVLLFEMNVVPAISAESNSVKSSTTAAHSANASP
                     VTQSSDAADADSPQAREERLAWFKQAKYGMFIHWGLYAIPAGEWKGKRCPGLGEWIMN
                     RCRIPVKEYEQLAKQFNPVDFDADRWVQLAQDAGMKYIVITAKHHDGFAMFGSKASPY
                     NVVDATPYRRDVLKELAEACRRHNMPLGFYYSQSQDWHEPGGAGNDWDFGPDQDANKK
                     ELKPYDQYLQGKAEPQVKELLTNYGPVALIWFDTPRMMTKQRAQRFTNLVRSTQPKTL
                     IDGRLGDKGDYVSTGDNVIPSGRSDQYWEVPATTNHTWGFRKDDTDWKAPGDILFKLV
                     DAVSKGGNYLLNVGPMANGVIPQEAQDNLRTVGSWLKKNGEAVYGAGPTPWGNEFGEP
                     SAKGEKDLRGNPLVLAHDEWRVTTRPGKLYFTFFQEPRVPFELPPMKNKIVRAYQLED
                     RKPVPIHQENGKTNIEVPRPMWDPMATTIVVEIEGGTVEQ"
BASE COUNT          466 a          567 c          632 g          373 t
ORIGIN      
        1 gaattctcat tgacctttac ctccggagag atcgatggga aacagacaag ctgtccctgg
       61 ccaggacgcc aaaccagaac cttttggtgc gcaccagcgg accgggcttc gtccgaatgt
      121 tacgcattat agtgttaccg aatgtaacac atacgattgt accgagcgca aggaaatttc
      181 catcttcatg tcgatccagt tgtggcagcg ccgtctgtcg gacaagtggg tgacccgacg
      241 cggcgaatga gtgcatggcg gctggcgaaa gcatccaaag ttctgctcac cgtagcgtgt
      301 gtctcaatat atgagacgca tatctcattc attgaccttg ccgcgtgacg gtgggtaaga
      361 tgctcaggat gaaacgcgcc ccctttcctc tcctggttct tgtcctgttg ttcgagatga
      421 acgtagttcc ggcaatctcc gcggaatcga attccgtgaa gtccagtacg accgccgcgc
      481 attcagcaaa tgcttcgccg gtgacgcaaa gttccgatgc agcagacgcc gactcgccgc
      541 aagcacgcga ggaacgcctg gcctggttca aacaagcgaa gtacggcatg ttcatccatt
      601 gggggctcta cgcgattcct gcgggcgaat ggaagggcaa gcgctgcccg ggcctgggcg
      661 agtggatcat gaaccggtgc agaatcccgg tgaaggaata cgagcagctg gcgaagcagt
      721 tcaacccggt ggatttcgac gcggaccggt gggtgcaact ggctcaggac gcgggcatga
      781 agtacatcgt gatcacagcg aaacaccatg acggctttgc gatgttcggt tcgaaggcaa
      841 gcccgtacaa cgtggtcgac gctacgccct atcgccgtga cgttttgaag gaactggcgg
      901 aggcatgccg ccgccacaac atgccgctag gcttctatta ctcgcagtcg caggactggc
      961 atgagcccgg gggagcggga aacgactggg acttcggtcc ggaccaggac gcgaacaaga
     1021 aggagctgaa accatacgat cagtatctgc agggcaaggc tgagccacag gtgaaagaac
     1081 tgctgacgaa ctatggaccc gtggcactga tttggttcga caccccgcgc atgatgacga
     1141 agcagcgagc ccagcggttt accaacctgg tgcgatcgac gcagccgaag acactgatcg
     1201 acggccgcct cggggacaag ggcgactatg tctcgaccgg cgacaacgtc attccgtcag
     1261 gcaggagcga tcagtactgg gaagtgccgg ccacaacgaa ccacacgtgg ggcttccgca
     1321 aggatgacac cgactggaag gcgccgggcg atattctctt caagctggtc gatgccgtga
     1381 gcaagggggg taattatctt ctgaatgtcg ggcccatggc gaacggggtg atccctcagg
     1441 aggcgcagga caatctgcgc accgttggaa gctggctgaa gaagaacggc gaggcggttt
     1501 acggcgctgg accaacgccg tggggtaacg agttcggcga gccgagcgcg aagggcgaaa
     1561 aggacctgcg cggcaatccg ctggtgttgg cgcacgatga gtggcgcgtt acgacccgcc
     1621 cgggaaagtt gtactttaca ttttttcagg agccgcgcgt acctttcgaa ctcccgccta
     1681 tgaagaacaa gatcgtgcgc gcctaccaac tggaagacag gaagcctgtg ccgatccacc
     1741 aggagaacgg gaagacgaac atcgaagtgc cgcgtcccat gtgggatccg atggcgacca
     1801 cgattgtggt ggaaatcgaa ggcggaaccg tggaacagta aaacctgcgt atccaccctg
     1861 gcgctccgcg ggccgggacg cccgcggaga ttgtctcatc gccgatcgaa ccgctacgcg
     1921 gcacttacgg gaacaggcgc ctccctcggt tcgatcacgc caaggctctt cacaacctgg
     1981 aactgaccgc cgcgagcctg ggcaatgtac atgttcatgc gcacatggtg ttgtccgg
//
LOCUS       DASZYN010000578         4062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_159086, whole genome shotgun sequence.
ACCESSION   DASZYN010000578 DASZYN010000000
VERSION     DASZYN010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159086"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VHE33_15030"
     CDS             <1..325
                     /locus_tag="VHE33_15030"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HVT98816.1"
                     /translation="VKSLYITENGCSSDDVLRPEAPNAAGQVLDSDRVLYLRNYLTQM
                     QRAAGEGVPLHGYFLWSLLDNFEWASGYSKRFGITYVDFETQKRVPKMSYDFYKEVIR
                     SNSVV"
     gene            complement(549..1157)
                     /gene="yihA"
                     /locus_tag="VHE33_15035"
     CDS             complement(549..1157)
                     /gene="yihA"
                     /locus_tag="VHE33_15035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HVT98817.1"
                     /translation="MKTLYPKFVLSATAAAHFPKPGPPEVAFLGRSNVGKSSLINALL
                     GEKQAHVSSTPGRTRAINFFALATAPWREKPGQHPQLLFADLPGYGYAKISKSISADW
                     PKFIDPYLRERPTLALCVCLVDSTIPPKASDAQLFEFLESAGRNYLIVGTKADRLSGN
                     GRAKALAALRQAFGDVPLLFCSAKTGVGVRELWASIRESVQS"
     gene            1222..1668
                     /locus_tag="VHE33_15040"
     CDS             1222..1668
                     /locus_tag="VHE33_15040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVT98818.1"
                     /translation="MRVIARCAVLSVLLLVGSGLPVRAQSSESAIRRVLADQQSAWNR
                     GDIVAFMQGYSDSPQTTFIGKTIQHGYQMILARYRRKYGSRAAMGELMFSDLDVRMLG
                     DEHAVVTGHFHLTRAAAGGGDAAGMFSLIFEKEKDGWKIILDHTSV"
     gene            2089..3387
                     /locus_tag="VHE33_15045"
     CDS             2089..3387
                     /locus_tag="VHE33_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98819.1"
                     /translation="MKICKSFAVLAFLFAAVTAFADPTVASPANGSTVSSPFNLSASA
                     SSCSSHSISAMGYSFDNSPSTTFVYSNSFSASVAASAGAHVLHVKSWGVGGGGCVTDV
                     AITVSGTTPPAATSPDVAVSSPGAGASVYSPFTLIASSPTCQGHSTAAMGYSFDNSPN
                     TAIFYGTAMNGPVSAANGAHVLHVKAWGNGGGACNTDVPINVTAPANAAVLPSSAISV
                     SNIHNLSNWTQSNDSGAMGWSTGAQGLVGSPSRSGSAREFVTSFSNNGNERYWVSFGD
                     DNVSTNFLYDTWVYVQSPNTQLANIEMDMNQTMPNGQTAIFGFQCDGWNGTWDYTKNA
                     GTPQAPVDTWVKSNQHCNPREWSVNTWHHVQVAYSRDNSGNINYQTVWLDDVPQPINV
                     TVNSAFALGWAPSLLTNFQVDGIGDGQAIVFMDNLTVYRW"
     gene            3423..3842
                     /locus_tag="VHE33_15050"
     CDS             3423..3842
                     /locus_tag="VHE33_15050"
                     /inference="COORDINATES: protein motif:HMM:NF039927.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5946 family protein"
                     /protein_id="HVT98820.1"
                     /translation="MPSEQELFDELSFYTLAHGDPAFIHENAADAWRAQQANETTKPM
                     AVVFAVMGLYLYIEKGFTGKQVQRAHMRMARGRRSWPQLALPTERGRITVADVVAAKP
                     GMERDAMIRRWCEAVWETWQESRPQIMELARAELGVD"
BASE COUNT          831 a         1120 c         1296 g          815 t
ORIGIN      
        1 cgtgaagtcg ctctacatta ccgagaacgg ctgctcgtcg gatgacgtcc tgcgccccga
       61 ggcgccgaat gccgcgggcc aggtcctcga ctccgaccgc gtcctctacc tgcgcaacta
      121 cctcacccag atgcagcgcg cggcaggcga aggcgtaccc ctccacggct acttcctctg
      181 gagcctgctc gacaacttcg aatgggcctc gggatattcg aagcggttcg gcatcacgta
      241 tgttgacttc gaaacgcaga agcgtgtacc gaagatgtcg tacgacttct acaaagaggt
      301 gatccgctcc aactccgtgg tgtgaggaca gcgttcagtt gtcagcgttc agcgttccgt
      361 tgtcagggca atcgtcaaag gcaaatgccg aggctttctg ggagcctggt gtggtgtttc
      421 cgaaatttct ttatgcggaa gagaagcagg tccctccgct tccccttcgc tacgctcaga
      481 gtccggtcgg gatgacaagg taaaggaagt tcagaaacac cacaccagga cgcggacgct
      541 cgagggggtc acgactggac ggattcccga atcgaggccc agagctcgcg gacgccgacg
      601 ccggttttcg cggaacaaaa gagcaaagga acgtctccga aggcctggcg cagagcggcg
      661 agtgctttgg cgcggccgtt tcctgagagg cgatctgctt tggtgcccac gatcaggtag
      721 ttgcggcctg cgctctcaag aaattcaaag agctgtgcgt cgctggcttt cggcggaatc
      781 gtgctgtcga cgaggcagac acacagagcg agcgtgggcc gttcgcgcag ataggggtcg
      841 atgaatttag gccagtccgc cgagatggat ttggaaatct tcgcgtatcc gtagccaggc
      901 agatcggcaa agagcagttg cggatgctgg ccgggttttt cgcgccaggg agccgtggcg
      961 agggcaaaaa aattgatggc gcgcgtgcgg ccgggcgtgg aggagacgtg ggcctgcttt
     1021 tcgccgagga gtgcgttaat caggctcgat ttgccgacat tcgaacggcc gagaaaggcg
     1081 acctccggcg ggcccggctt tggaaaatgg gcggcggccg tcgccgaaag aacaaatttg
     1141 ggatacaaag tcttcatcgc ttcgagcccc agtctaacga aacgctcgca ggcgcacatc
     1201 gtttgttgaa agggatcgtc gatgagagtg attgcccgct gtgctgtcct gtctgttctg
     1261 ctgctggttg ggagcgggct tccggtccgc gctcagtcga gcgagagcgc gatccggcgc
     1321 gtactggccg atcagcagag tgcgtggaac cgcggcgata ttgtcgcgtt catgcaggga
     1381 tacagcgact ctccgcagac gaccttcatc ggcaagacga tccagcacgg atatcagatg
     1441 atcctcgcgc ggtatcggcg gaagtacggt tcacgcgcgg cgatgggcga gctgatgttc
     1501 agcgatcttg atgtgcgcat gctgggagat gagcatgcag tggtcaccgg acattttcat
     1561 ctgacgcgag ctgcagcggg aggcggcgat gcggctggga tgttctcgct gatcttcgag
     1621 aaagagaaag acggctggaa gatcatcctc gatcacacaa gcgtgtgaga aggagatccc
     1681 ccgcaagagg gacgcgttct gcggctcaaa cgacacaggt cctgggagcc ctcacagcga
     1741 gctgaaaaat ttccacggcg gaaagggcga atccggtaag aagttcccgt tttcgcaaaa
     1801 acgttgcccg agtgttacca aggttttcac tttcgggcaa cttttttctt gcgttcgatt
     1861 gaggtgaagg tactatgcgg tcagtaatta acttgactcc tgtgatcggg cctaagtagt
     1921 taccgttgta accacttacg cctgcggctt ctggtggaaa gttttccaga aactgcgccg
     1981 tgaacccctg cgcgaccggg gttccagcat gcacgtggcc cggggggcca ggcattaccg
     2041 cacgctggca cgacctcatt ctctttttta gtaaggtttc gcgcactcat gaagatttgt
     2101 aagagttttg ctgttttggc cttcctgttc gccgccgtca ccgccttcgc cgatcccacg
     2161 gtcgcttcgc ccgctaacgg atccacggta tcctcccctt tcaatttgtc tgcgagtgcg
     2221 tcgtcgtgct catcccattc catttcggcg atgggctact cgtttgataa cagcccaagt
     2281 acaacgttcg tctacagcaa ttctttcagc gccagcgttg cagcctcggc cggagctcac
     2341 gttctgcacg tgaagtcgtg gggtgttggc ggcggcggtt gtgtgacgga tgtcgccatc
     2401 accgtcagcg gcaccacacc accggctgcg acctcgccgg atgtggcggt tagcagccct
     2461 ggggcgggag cgagcgtcta ttcgccgttc actctgatcg ccagttcccc gacctgccag
     2521 ggacactcga ccgcggcgat gggctattcg ttcgataaca gccccaacac tgcgattttc
     2581 tacggaaccg cgatgaacgg acccgtgagc gctgcgaacg gagcgcacgt gctgcacgtg
     2641 aaagcgtggg ggaatggcgg aggcgcatgc aataccgatg tgccgatcaa cgtgacagca
     2701 ccggccaacg ccgcggtgct tccctcgtcc gcgatcagtg tttcgaatat ccataacttg
     2761 tcgaactgga cgcagagcaa cgacagcggg gcgatgggat ggtcgacagg ggcgcagggg
     2821 ctggtggggt cgccgtcacg cagcggctcg gcccgtgaat tcgtgaccag cttcagcaac
     2881 aacggcaacg agcgctactg ggtgtcgttt ggcgacgaca atgtttcgac gaattttctg
     2941 tatgacacct gggtttacgt tcagagcccg aacactcagc tggcgaatat cgagatggat
     3001 atgaaccaga cgatgcccaa cggacagacg gcgattttcg gcttccagtg cgatgggtgg
     3061 aacgggacct gggactacac gaagaatgcc gggacaccgc aggcgccggt ggacacctgg
     3121 gtgaagtcga accagcactg caatccgcgg gaatggagcg tgaacacgtg gcatcacgtg
     3181 caggtggcct actcacgcga caattcgggc aacattaact atcaaacggt gtggctcgat
     3241 gacgtgccgc agccgatcaa tgtgacggtg aactcggcct tcgcgctggg ctgggcgccg
     3301 agtctgctga cgaacttcca ggtggacgga atcggcgacg gccaggcgat cgtgttcatg
     3361 gacaacctga cggtttaccg ctggtaagat cagggaccgg atccaggctg gagacggtcc
     3421 ctatgccttc cgagcaggaa ctcttcgacg agctgtcgtt ctatacgctg gcgcacggcg
     3481 atccggcgtt tatccacgag aacgccgcgg atgcatggcg ggcacagcaa gcaaacgaaa
     3541 caacgaaacc catggccgtg gtgttcgcgg tcatgggttt gtacttatat atcgagaaag
     3601 gcttcaccgg gaaacaggta cagcgggcgc atatgcgcat ggcgcgcggg cgacggtcgt
     3661 ggccgcagct cgccttgccc acggaacgcg ggcgtattac ggtcgcggat gttgtggctg
     3721 caaaacctgg gatggagcgc gatgcgatga tccgcaggtg gtgcgaagcg gtttgggaga
     3781 cctggcagga gagccggccg cagatcatgg aattggccag ggcggaactg ggcgtggatt
     3841 gatgcggcgc ggcttgacat tggtccgcga cggcctgagt ctcgtccaga ggacagcatg
     3901 actgcacaat tccagccgac accgacgaac ctgtttctgc gattttcgcg cgacaagctg
     3961 ttgaatcagt actggccgcg gctgcgcaaa tgtgtcgagc cgctgacgga cgagcaaatc
     4021 tggtggcggc cgaacgcttc gagcaactcg atcggaaacc tg
//
LOCUS       DASZYN010000579         1858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_34029, whole genome shotgun sequence.
ACCESSION   DASZYN010000579 DASZYN010000000
VERSION     DASZYN010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34029"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1858
                     /locus_tag="VHE33_15055"
     CDS             <1..>1858
                     /locus_tag="VHE33_15055"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT98821.1"
                     /translation="LRSLPVADPSHLVRIGDRDDCCYWNTVQNQDGDFDLFSYDAYRQ
                     MKAAAPEFEQLAAVQAGGASFSVREGSQPSVALRAEYVSGNYFTTLGVGAFAGRPLIE
                     NDDREGAVPVLVLSYRAWQTLFGGDAALVGSTVNVEGHTFTIAGIAPPGFFGDRVAPV
                     PPDAWAPLAAEAVMEGANSALLDKDEDWLYPLGRLRAGVARGPLQAKLSAVLRQWLMS
                     RPAYTDHGGAALIPKQHVVLADAGGGIQKLQQQTGKGLRMLMILSGVVLLIACANIAS
                     LLLARNAARRPEIAARMALGAGRARVVRQILTESALLSAMGGAAGLAIAYFGSHAMLR
                     LAFPLAKNMTVDANPSWPVLGFALTVSLLTGIVFGAAPAWASSHAMPAEALRGANRAT
                     RDHSSLPQRTLVVLQVAWSVILLAGAFATTKSLSNLEHQDFGIVTANRYVLQLDPKAA
                     GYSTAQLPALYRQIEERFGALPGATHVALARYLPMGGNMWGSCVIQEGHPQPGPSDHC
                     FAIWDRVSAGFLNTLGVPLVRGRDFTASDTQTTTAVALVNEAFARQFFPGENPIGQHF
                     GIDQARNSGRFEIVGVFANIMMANARGDDRPVFLRPATQQLSGFGSAQADAGE"
BASE COUNT          314 a          585 c          612 g          347 t
ORIGIN      
        1 gctgcgatcg ctgccggttg ccgaccccag tcatctggtc cgcatcggcg acagggacga
       61 ctgctgctac tggaacacgg ttcagaacca ggacggcgac ttcgatctgt tttcctatga
      121 cgcttaccgg caaatgaagg ccgcggcccc ggagttcgag caactggctg ctgtgcaggc
      181 gggaggcgcg agcttcagcg tgcgggaggg ttcgcagccg agcgttgcat tgcgagccga
      241 gtatgtctcg ggaaattact tcacaaccct cggcgtgggc gctttcgcag gaaggccgct
      301 gatcgaaaac gacgatcgcg agggcgcggt tccggtgctc gtactgagct accgggcatg
      361 gcagaccctg ttcgggggcg acgccgctct cgtgggatcg accgtcaatg tagagggcca
      421 cacattcacc atcgccggga ttgcgccgcc cggtttcttt ggcgaccgcg tcgctcccgt
      481 tcctcccgat gcatgggcgc cactcgcagc cgaggcggtg atggaaggcg ccaactcggc
      541 gctcctcgac aaagacgagg actggctcta tcccctggga cgcttgcgcg cgggcgttgc
      601 gcgcggaccc ctgcaggcaa aattatcggc ggtcctgcgg cagtggctga tgtcccggcc
      661 tgcctatacc gaccacggcg gcgccgcgct gattccgaaa cagcatgtgg tcctcgccga
      721 tgccggtgga ggcatccaga agctgcaaca gcagaccggc aagggcctgc gcatgctcat
      781 gattctttcc ggagtcgtgc tcctgatcgc ctgcgccaac attgcgagcc tgctgctggc
      841 acggaacgcg gcgcggcgtc ccgaaatcgc ggcgcggatg gcactcggag cgggacgcgc
      901 acgtgtagtg cggcagattc tcactgagtc agccttgctg agtgccatgg gcggtgcagc
      961 gggactggcc atcgcatatt tcggctcgca tgcgatgctg cgcctggcct ttccgctggc
     1021 aaagaatatg accgtcgatg ccaatccgtc gtggcccgtg ctcggattcg cgctgacggt
     1081 ctcgctgctg acgggcatcg tctttggcgc ggctccggcg tgggcgtcct cgcatgcaat
     1141 gccggccgaa gccctgcgcg gtgcgaaccg ggcgacccgg gaccactcgt ctttgccgca
     1201 gcgaacgctg gtggtgctgc aggtggcatg gtcggtgatc ctgctggccg gtgccttcgc
     1261 gacaacaaag tcgctcagca atctggaaca ccaggatttc ggcatcgtga ctgccaaccg
     1321 ctatgtgctg cagctcgatc caaaggccgc cggctattcg acggcgcagc ttccggcgct
     1381 gtaccgccag attgaagaac ggttcggcgc attgcccgga gcgacccatg tcgccctggc
     1441 ccgctatctg cccatgggcg gcaacatgtg gggctcgtgc gtgatccagg aaggtcaccc
     1501 gcagccgggc ccgtcggatc attgctttgc catctgggac agggtgagcg caggattttt
     1561 gaacacgctg ggcgtgcctc tggttcgagg ccgtgacttc acggccagcg atacacaaac
     1621 cacaacggct gtggccctgg tgaatgaggc gttcgcgcga cagtttttcc ccggcgagaa
     1681 tcccatcggc cagcacttcg ggatcgacca ggctcgcaac tccgggcgtt tcgaaattgt
     1741 gggcgtcttc gcgaacatca tgatggccaa tgcgcgcgga gacgatcgtc ccgtatttct
     1801 gcgccccgca acccagcaac tgagcggatt cggcagcgca caagccgatg cgggagag
//
LOCUS       DASZYN010000580         5502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_478626, whole genome shotgun sequence.
ACCESSION   DASZYN010000580 DASZYN010000000
VERSION     DASZYN010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478626"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="VHE33_15060"
     CDS             complement(<1..1098)
                     /locus_tag="VHE33_15060"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HVT98822.1"
                     /translation="MTAFRPLSRGLSRRNFARTLAGTIASLRFAGLPALSATQPPHYP
                     FEEVPPSVSGITWVHTAGKSPQKYLPETTGAGCAFLDYDNDGWMDIYLVNSGKCDFYD
                     PSPPLRNALYRNNRDGTFTDVTEKAGVPGGGYGQGVAVGDYNRDGFPDLYVTQYGRSI
                     LYRNNGDGTFTDVTDAAGVAAPGWSSSAVWFDYDNDGWLDLFVCQFVEFSKTKNLPCV
                     TYNNRPGYCVPRLYKPAASWLFRNNQDGTFTDVSKLSGIADHLGKAWGAVAADLNNDG
                     RLDLFVSNDTTSNFLFMNRGAGKFEEIGALAGVAFSQAGRARSGMGVDAADVNQDGWM
                     DLFVANIDHEEYSLYQNNHDETFDDEAASMGI"
     gene            complement(1095..2045)
                     /locus_tag="VHE33_15065"
     CDS             complement(1095..2045)
                     /locus_tag="VHE33_15065"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT98823.1"
                     /translation="MSQFPRIFSFLILLPLALQGSSVLGQKAVPADDAVSAEHAASAA
                     EAGRCSEALPTLTRTVAHLADKALQKRVGLDGVRCATLLQRWDDLLQLVHVLNQKLPG
                     DPEVLYLSVHAYSDLSNQAAQKLAQTAPHSLPALELDAEASEMQGRWDQAEKDYRTIL
                     QKNPHYPGIHFRLARLLLSKPNPAPNFQDEAKKELQEELSIDPSNAGAEYVSGELARQ
                     AQDFAGAVHHFTRATELDDNFADAWLGLGMSLLSQKQYAPAVAPLKTAVRLEPANPAA
                     HYALATAYARTGNKEAAEHEFALQKQAAQDSGGQGDKAPQ"
     gene            complement(2098..5415)
                     /locus_tag="VHE33_15070"
     CDS             complement(2098..5415)
                     /locus_tag="VHE33_15070"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT98824.1"
                     /translation="MRRMMLWGSILVALAATQLSCPLQAQQITASITGTVIDAGGAVI
                     NSATVTATDIERGTAYTTKSADGGIFTFARLPIGTYDVKVEAPGFQTAVQSHLTLVLN
                     QTARLEFKMSVGAVTNTVQVTSEAPELQSETTEVSTLVDSRAVSNIPLATRNYVELTL
                     LSPGSVNPDNSTFNNGDNTASGGRPYINGNREQSNNFILDGMDNNQTSDNLLAFTPSP
                     DAIQEFNLITANAPAEFGNFQGGIINASIKSGTNQFHGDVWEFLRNDVLNANQWENKF
                     NGPGNELPRATLRWNMYGGTLGGPILKDKLFFFLDYQGQRFDHPATSNFITVFTNAER
                     GGDFGALLPTTQLKDPTTGQNYDQNQIPVGQRDSVATKLFASSYYPTPINNNQVNNAV
                     NVYTQAFNTDQGDLKIDYNITARDHFDGRYSQEYQNDPASNSLLILGNTFTHAPIHNV
                     VGTWTHTFSPNLLNELRFGTSWITLLTGTTFDPKIGNLGTDLGIANANSVGPGLLLMG
                     FAGGTAPEPGSGGALTNIGSSVVDQDFADTVIQFDDGLNYTRGKHVIKAGFQMWRYRI
                     NTFYSGNSGEYGSILFGGSFSGDAGADFFLGYPEATGKGVSGGGTWHQFSWTYAGYGQ
                     DDWRITPTLTLNLGLRYEAHTPWVELNNLQDNLDLVTGQIEYAGKDGNSRSLYNSVYG
                     LPDFEPRFGFAWSPAALHNKTVVRGAYTISSYLEGTGTNLRLARNPPFTPTEVTATYS
                     GPTYRTEQGPGGSVSGDPFAGAVMYVWDKTVQPAMDQQWNLTIQNAFTANTALQVGYV
                     GQHGTHLMVPTPYSQERLVNGTAQPGTYFQGNPSLLADISTVSGTSSTGYMHYDALQS
                     VLEHRLSNGLEGQVAYTWSHCLTNNSGYYGTWGAATQATPASPYYQNLYDPAGDYASC
                     YYNSKHILSTYATYSLPVGRGRKYGANMNRAVDAVVGGWQASTIVSLHSGFPLAVYNA
                     TDTSGTGSRGPRPNCGTQHVYGRKSSFDSSGVFQGYVWMDPSGFSEPAAGTFGNCPAQ
                     GPDVGPGYENADIGVMKNFHITENKYFQFRSDFLNAFNNVQLGHPDTNYPNPTSFGLI
                     NTSQPARNIQFALKFYY"
BASE COUNT         1112 a         1443 c         1718 g         1229 t
ORIGIN      
        1 gatgcccatg gatgcggctt catcgtcgaa ggtctcgtcg tgattgttct gatagaggga
       61 atattcttca tgatcgatgt tggcaacgaa gagatccatc cagccgtcct ggttcacgtc
      121 ggcggcatcg actcccatac cggagcgcgc cctgcctgcc tggctgaagg cgacgcccgc
      181 gagcgcgcca atctcttcga atttgcctgc cccgcggttc atgaagagaa aattcgaggt
      241 ggtgtcgttg gagacaaaga gatcgagtcg gccgtcgttg ttgaggtcag ccgctacagc
      301 gccccaggcc ttgcccaggt ggtcggcaat gcccgacaat ttgctgacat cggtaaacgt
      361 gccatcctgg ttattgcgaa aaagccagct cgctgccggc ttatagagac ggggcacaca
      421 atagcccgga cggttgttat aggtgacgca gggaagattc tttgttttcg agaactcgac
      481 aaactgacag acaaacagat cgagccatcc gtcgttgtca tagtcgaacc agacggcgct
      541 cgatgaccag cctggcgccg ccacgccggc cgcgtctgtt acatcggtga aagttccgtc
      601 tccgttgttg cgatacagga tgcttcttcc atactgcgta acgtagaggt cgggaaagcc
      661 gtcgcggttg tagtcgccaa cggctacgcc ttgtccataa ccgccaccgg ggacgcctgc
      721 cttctcagtg acgtcggtaa acgttccatc ccggttgttg cggtaaagcg cgttgcgcag
      781 cggcgggctg ggatcgtaga aatcgcattt gccactgtta accagataaa tatccatcca
      841 gccgtcgtta tcgtaatcaa gaaatgcgca accggcgccg gtagtttccg ggagatactt
      901 ctgcggcgac ttacccgcgg tatggaccca ggtaatgccg ctgacagaag ggggcacttc
      961 ctcgaatgga tagtgaggag gctgggtggc ggagagtgcc gggagaccgg caaaacgcag
     1021 agaagcaatg gttccggcga gggttcgggc aaaattgcgg cgcgacaatc cacgcgagag
     1081 tggcctgaaa gctgtcactg cggcgctttg tccccctgac cgccggaatc ctgcgctgcc
     1141 tgtttctgca gggcgaattc atgctccgcg gcttctttgt tgccggtacg ggcgtaagcc
     1201 gttgcgagag catagtgtgc cgccggattt gccggttcca gccggacagc ggttttgagt
     1261 ggggcgaccg ccggcgcata ctgcttttgc gacagcagag acattcccag tcccagccag
     1321 gcatcggcga agttatcgtc cagttcggtc gcgcgggtaa agtgatggac tgccccggcg
     1381 aaatcctgcg cctgacgagc cagttctccc gagacatatt ccgcgcctgc gttcgaggga
     1441 tcgatgctga gctcttcctg taactccttc tttgcctcgt cctggaaatt gggcgccggg
     1501 ttgggtttgg acagcagcaa tcgagccaga cggaagtgga tgcctgggta gtggggattt
     1561 ttctggagaa ttgtgcggta atccttctcg gcctgatccc accggccctg catttcactg
     1621 gcttcggcat ccagttcgag tgcggggagc gaatgcggcg cggtctgcgc cagcttctgc
     1681 gccgcctggt tggatagatc ggagtaagca tgaaccgaga ggtagagcac ctcgggatca
     1741 ccggggagtt tctgattgag gacatgcacc agctgcaaaa gatcatccca ccgctgcagc
     1801 aaggtggcgc agcgaacacc atcgagaccg actctcttct gcagcgcttt gtccgccagg
     1861 tgagcgactg ttcttgtgag cgtgggcagg gcctctgagc atcggcccgc ttcggcggcc
     1921 gacgccgcgt gctcggcact cactgcatcg tctgcaggaa ccgccttctg gcccaggaca
     1981 gaggaaccct ggagggccag aggaaggaga atcagaaagg aaaagatccg gggaaattgg
     2041 ctcataatca gcggaggcaa gcgcacggag cgcctgcccc cgtcgccttc agttccccta
     2101 ataatagaac ttcagagcaa actggatgtt cctcgccggt tgagaagtat tgatcagtcc
     2161 aaacgacgtg ggattggggt agttggtatc ggggtggccc agctgcacgt tgttaaatgc
     2221 gttcaggaaa tcactgcgga actggaagta tttgttttcg gtgatgtgga agttcttcat
     2281 cactccgatg tccgcatttt catagcctgg acctacgtca ggaccctgcg cgggacaatt
     2341 gccgaaggtc ccggcggccg gctcggaaaa gccgctggga tccatccata catatccctg
     2401 gaatactccg ctggagtcaa agctggattt tcgtccgtaa acgtgctggg ttccgcaatt
     2461 cgggcgaggg cctcgggacc cggttcccga tgtatcggtt gcgttataga cggcaagcgg
     2521 gaagccgctg tggagagaaa cgatggtgga ggcctgccac ccgccgacca cggcatcgac
     2581 ggcgcggttc atgtttgcac catatttcct gcctcggccg acgggcagag agtaggtggc
     2641 gtaagtggaa agaatatgtt tggagttgta atagcagctg gcatagtcgc ccgcaggatc
     2701 gtagagattc tggtaatagg gtgacgccgg tgtggcctgc gtggccgcgc cccaggttcc
     2761 gtagtagccg ctgttgttgg tgaggcagtg cgaccaggta taggccacct ggccttcaag
     2821 gccgttggaa agacgatgtt ccagcacgct ctgcagggca tcgtaatgca tgtagccggt
     2881 ggacgacgta ccggacaccg tggagatatc cgcaagcagc gatggatttc cctggaaata
     2941 ggtgccgggc tgagcggttc cattcacgag ccgttcctgg gagtagggag tgggaaccat
     3001 caggtgggtt ccatgctgcc cgacatagcc aacctggagt gcggtgttgg cagtgaaggc
     3061 attctgaatg gtgaggttcc actgctgatc catggccggc tggacggtct tgtcccatac
     3121 gtacattacc gcgccagcga agggatcacc gcttacgctg cccccgggac cctgctcggt
     3181 cctgtaagtc gggccactgt aagtcgcagt cacttcggtg ggagtaaagg gagggttgcg
     3241 ggcgagacgc agatttgttc ctgtcccttc aaggtaagac gatatggtat atgctccgcg
     3301 gactacagtt ttgttgtgca gcgcggccgg cgaccaggcg aaaccgaagc gaggctcaaa
     3361 gtcaggcaat ccataaacgg agttataaag cgaccggctg tttccatctt ttcccgcgta
     3421 ttcgatctga ccggtaacca gatcgagatt gtcctgaaga ttgttgagct caacccaggg
     3481 agtgtgggct tcatagcgca atccaagatt cagagtcaga gtgggagtta tgcgccagtc
     3541 gtcctgtccg tatccggcat aggtccagga gaactgatgc cacgttcctc ccccgctgac
     3601 ccctttaccg gtcgcttcgg gatagccgag gaagaagtcg gctccagcat cgccggagaa
     3661 tgatccacca aacaaaattg atccgtattc accactattg ccgctgtaga aggtgttgat
     3721 ccggtagcgc cacatctgga agcccgcttt gatgacatgc ttcccgcggg tgtagttcaa
     3781 accgtcgtcg aactggatga ccgtatccgc gaaatcctgg tcaacgacgc tgctgccgat
     3841 gttggtgagg gcgccgccgg agccgggctc aggcgctgtg ccgccggcaa accccatcag
     3901 gagcaggccc ggtccaacgg aattggcgtt ggcgatgcct aaatcagtcc caagattgcc
     3961 gatcttcggg tcaaacgtgg tgccggtaag gagcgtgatc cagcttgtac cgaagcgcag
     4021 ttcgttcagg agattggggc tgaaggtgtg agtccaggtg ccgacaacat tgtgaatggg
     4081 cgcgtgagta aaggtgttgc ccagaatcaa aagcgaatta ctggcgggat cgttctgata
     4141 ttcctgggaa tagcgtccgt caaagtgatc cctggcagta atgttgtaat caattttgag
     4201 gtcaccctga tccgtattga acgcctgtgt atacacgttg acggcgttgt tgacctgatt
     4261 attgttgatg ggcgtcggat agtaggaaga ggcaaacagt ttggtcgcga cactatcgcg
     4321 ttggccaacc gggatctggt tttggtcgta gttctgcccg gtagttgggt ccttgagctg
     4381 ggttgttggc agcaaggcgc cgaaatcgcc accccgctct gcgtttgtga agaccgtgat
     4441 gaagttgctg gttgcaggat gatcgaagcg ttgcccctga taatccagga agaagaatag
     4501 cttatctttc aggatagggc cgcccagggt accgccgtac atattccagc gcaacgtggc
     4561 tcggggtaat tcgtttccgg ggccattgaa tttgttctcc cactggttgg cgttcaacac
     4621 gtcgttgcgc agaaattccc agacgtcgcc atggaactgg ttggtgccgg acttaattga
     4681 ggcattgatg atgccgccct ggaaattgcc gaattcggcg ggcgcattgg cggtaatcag
     4741 attgaattcc tggatggcat cgggagacgg tgtgaaggcg agcaagttat ccgatgtctg
     4801 gttattgtcc atcccgtcga ggatgaagtt gttggattgc tcacgattcc cgttgatata
     4861 gggtctgccg ccgcttgcgg tgttatcgcc gttgttgaag gtcgagttat cgggattgac
     4921 gctgccagga gacaacaacg tcagttccac gtaattgcgg gtcgcaagcg ggatgttgct
     4981 gaccgcgcgt gagtcgacaa gggtgctgac ttctgtggtt tccgactgca gttcaggcgc
     5041 ttcggatgtg acctgcactg tgttcgtgac ggcgcctacg ctcattttga actcaaggcg
     5101 cgccgtctgg ttgagcacca gggtcagatg ggactggact gctgtctgaa agcccggcgc
     5161 ttcgactttg acatcgtacg tgccgatggg caggcgggca aaggtgaaga tgccgccgtc
     5221 ggcgcttttt gtcgtgtagg ctgtgccgcg ctcgatatcg gtggcagtca cggtggcgct
     5281 attgatcact gccccgcctg cgtcgatgac cgtaccggta atggatgcgg ttatctgctg
     5341 ggcctgcaaa gggcaagaga gctgggttgc ggcaagcgcc acgaggatag atccccacaa
     5401 catcatcctt ctcatactgg cctccagttg cttccgggga tacttgcggc atcttttcag
     5461 gcgatgccgg ggactgcact gaggagccca cttcctgggt cg
//
LOCUS       DASZYN010000581         2347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_159271, whole genome shotgun sequence.
ACCESSION   DASZYN010000581 DASZYN010000000
VERSION     DASZYN010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159271"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VHE33_15075"
     CDS             complement(<1..497)
                     /locus_tag="VHE33_15075"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HVT98825.1"
                     /translation="MILAGDVGGTKVHLALYSFEHRELVHVRDERFAANEYSGLQEIV
                     QHFLAESGSPEITAACFGVPGPVRGGRLRLTNLPWVLDARELSAGLNITHLFLINDLE
                     ANGYGIAELTPEQIFTLHPGDPASVGNRALISAGTGLGEGILVWNGKTHIPMASEGGH
                     SDFAAR"
     gene            complement(494..1294)
                     /gene="pgl"
                     /locus_tag="VHE33_15080"
     CDS             complement(494..1294)
                     /gene="pgl"
                     /locus_tag="VHE33_15080"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HVT98826.1"
                     /translation="MAKKIHVDYRVFPQQDALARATAEKFVQSIQTAVAARGVARIAI
                     SGGTTPKVVFALLADPNEPYRAAIPWDRLWIFWVDERCVPPDHPDSNYGAARDLLLSK
                     VPLPPDHVIRIEGELDPEEAAARYESAIRGHYRLEGAQGPVFDMIQLGMGNDGHCASL
                     FPHTDALHEMIRIAVANHVPQQKQSWRVTLTWPVINAARDVFFLIDGPAKADPLGHVL
                     QGPYDPETLPSQLIQPQNGRLLFLLDSAAAAHLPAPAADGTGTLEIAR"
     gene            complement(1373..>2347)
                     /gene="zwf"
                     /locus_tag="VHE33_15085"
     CDS             complement(1373..>2347)
                     /gene="zwf"
                     /locus_tag="VHE33_15085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HVT98827.1"
                     /translation="QIFRIDHYLGKETVQNVLVFRFANGIFEPIWNRNFVDHVQITAA
                     ESIGIEGRGPFYEKAGALRDVMQNHIMELLSFITMEPPASFAADAVRREKIKLWTSIK
                     PLNIFDCVRGQYGPGIVGDQMVKGYREEDRVDPKSQTETYAAVRLEIDNWRWAGVPFY
                     MRAGKRLARRVTEITIQFKQPPMLLFGTHMHGPCTDIQPNRITMRIQPDEGISLRFGA
                     KVPGPQMAVCPVVMDFNYAQAFGSSSANGYERLLLDAMLGDATLFAHRDGVETTWALY
                     TPVIDAWAKKVPQTFPNYAAGTWGPECGDELIGRDGHVWHSVDLPTKG"
BASE COUNT          444 a          629 c          834 g          440 t
ORIGIN      
        1 cgcgcagcaa agtcagagtg accgccttca gaggccatgg ggatgtgggt cttcccgttc
       61 cacacgagga tgccctcgcc gaggccggtg ccagcagaga tgagagcacg attgccaacg
      121 ctggcgggat cgccgggatg gagcgtgaag atctgttcgg gagtgagttc ggcgatgccg
      181 tagccgttgg cttcgagatc gttgatgagg aagagatgcg tgatgttgag gccggcggag
      241 agctcgcgcg catcgagaac ccagggaaga ttcgtcaggc gcaggcggcc accgcggacg
      301 ggaccgggaa cgccgaagca ggcggcggtg atttcagggc ttccactttc ggcgaggaag
      361 tgctggacga tctcctggag gccgctgtat tcgtttgcag cgaatcgttc atcgcgaacg
      421 tgaaccagct cgcggtgctc gaagctgtac agcgccaggt gaaccttggt gccgcctaca
      481 tcgccggcga gaatcatctt gcaatctcca gcgttccggt tccgtctgcg gcgggcgccg
      541 gcagatgcgc agcggccgcg gagtcgagca ggaacagcag ccgcccattc tgcggctgga
      601 tgagttggga gggaagggtt tcaggatcgt aaggcccctg cagaacgtgg ccaagcggat
      661 cggctttggc cggaccgtcg atgagaaaga acacatcgcg ggcggcgttg atgaccggcc
      721 acgtgagtgt gacgcgccag ctctgtttct gctgcgggac gtggttggcg accgcgatgc
      781 ggatcatttc gtggagcgca tcggtgtgcg gaaagagcga ggcgcaatgg ccgtcgttgc
      841 ccattccgag ctggatcatg tcgaagaccg ggccctgagc accttcgagg cggtagtggc
      901 cgcggatggc ggattcgtag cgagcggcgg cctcttccgg atcgagttca ccttcaatgc
      961 gaatgacgtg atcgggtggc aggggaacct tgctcagcag aaggtcgcgc gccgcgccgt
     1021 aattgctgtc gggatgatcc gggggaacgc agcgctcgtc gacccagaag atccagaggc
     1081 gatcccacgg aatggccgcg cggtatggtt cattgggatc agcaaggagc gcaaagacga
     1141 ccttaggggt ggtgccaccg gagatcgcga tacgagcgac gccgcgtgcg gcgactgcgg
     1201 tctgaatgct ctggacgaat ttttccgcgg tggcgcgcgc aagcgcatcc tgttgcggga
     1261 agacgcggta atcgacgtga atttttttgg ccattgccgg acgcgcggtg gaacggttac
     1321 cgaacgcaaa gcggcattct atcgccgcca ggacagtcgc gaaagccgaa tgctaaccct
     1381 ttgtgggaag gtccacgctg tgccagacat gaccgtcccg tccgatgagt tcgtcgccgc
     1441 attccgggcc ccaggtaccg gccgcatagt tggggaaggt ttgcggaacc ttctttgccc
     1501 aggcatcgat gacgggggtg tagagggccc aggtcgtctc cacaccgtca cgatgcgcga
     1561 agagggttgc gtcgccgagc atagcatcga gcagaaggcg ctcgtagccg ttcgcggagg
     1621 aggacccgaa agcctgcgcg tagttgaagt ccatgacaac gggacagacg gccatttgcg
     1681 gaccgggaac tttggcgccg aagcggaggg agatgccttc gtcgggctga atgcgcatgg
     1741 tgatccgatt aggctggatg tcggtgcagg ggccgtgcat gtgcgtgcca aaaagtaaca
     1801 ttggcggttg cttgaactgg atagtgatct cggtgacccg ccgcgcgagg cgtttgccgg
     1861 cgcgcatgta gaacgggaca ccggcccagc gccagttgtc gatttcgagg cgcacggcgg
     1921 cgtaggtttc ggtctgcgat ttgggatcga cgcgatcctc ttcgcgatat cccttcacca
     1981 tctggtcgcc aacgatgccg ggaccgtact ggccgcggac gcagtcgaag atgttgaggg
     2041 gcttaatgga ggtccacagc ttgatctttt cgcggcgaac ggcgtcggcg gcgaagctcg
     2101 caggcggctc catggtgatg aaggagagca gctccatgat gtggttctgc atgacgtcgc
     2161 ggagagcgcc ggccttttcg taaaaggggc cgcggccttc gatgccaatg ctttcggcgg
     2221 cggtgatctg cacgtggtcg acgaagttgc ggttccagat gggctcgaag atgccattgg
     2281 caaagcggaa gaccagcacg ttctgcacgg tctccttgcc gaggtagtga tcgatgcgga
     2341 agatctg
//
LOCUS       DASZYN010000582         2034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_222815, whole genome shotgun sequence.
ACCESSION   DASZYN010000582 DASZYN010000000
VERSION     DASZYN010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222815"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VHE33_15090"
     CDS             complement(<1..482)
                     /locus_tag="VHE33_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98828.1"
                     /translation="MHRLLHRNPHAAVLVLASLAAGFCLPGLSAQTPAPPPAVDAAAL
                     VRRAVANHLAAEATHHPQRFILHKKDERRDYMQAIIETKQGDVALAIAANGAALNPAT
                     HQAQIDRLDKLDADPDLQEHRRKREAEDTERANKLLRMLPDAFLYHYDATVPCIVTTQ
                     P"
     gene            667..1170
                     /locus_tag="VHE33_15095"
     CDS             667..1170
                     /locus_tag="VHE33_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98829.1"
                     /translation="MKFRVWAPLMAVVLGLVFAAHAQDHVRGRKYKAPPPTCKVTVTV
                     IRGYNGKPLENASVVFHPIKDGKDTGGMELKTDEDGKVTLDMIPVDEDLRLQVLATGF
                     QTFGHDYDLTGTSKEIEVRMNRPQRQYSIYESHPGNNAGQATTEPQGDQKDKDKDKPQ
                     QDQKPHD"
     gene            1486..>2034
                     /locus_tag="VHE33_15100"
     CDS             1486..>2034
                     /locus_tag="VHE33_15100"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVT98830.1"
                     /translation="MAQAVERALQDQRHLIVEAGTGTGKTLAYLLPALRRCAATGHRI
                     IISTGTKNLQEQLFFKDIPFLESLLGPLRVCYMKGRANYLCRHKLFALRNQPILSGLE
                     EISQYQAIADWEKTTETGDRAEIDDLPEASALWSKLDARSEACLGQTCPDYGRCFITE
                     MRRRAAESDVVIVNHHLFFADMA"
BASE COUNT          394 a          719 c          580 g          341 t
ORIGIN      
        1 ggctgcgtgg tgacaatgca cggcaccgtc gcgtcgtagt ggtagagaaa cgcgtccgga
       61 agcatgcgca gcagcttgtt cgcccgctcc gtatcttcgg cttcgcgctt ccgccgatgt
      121 tcctggaggt cgggatcagc gtccagcttg tcgagtcgat cgatttgcgc ctgatgtgtt
      181 gcggggttca gcgccgctcc attcgccgcg atcgccaggg ccacatcgcc ctgtttggtt
      241 tcgatgatcg cctgcatgta gtcgcgccgc tcatccttct tgtgcaggat gaaccgctgc
      301 ggatgatgcg tcgcctctgc agccaggtga ttggccacgg cgcggcgaac cagagcagca
      361 gcatcgaccg ccgggggtgg cgcaggagtt tgcgccgaga ggcccgggag acagaatccg
      421 gccgcaagcg aagccaggac cagaactgcg gcgtgcgggt tgcggtggag gagtcggtgc
      481 atgcgcttac ggcactagca aagatggcgc ggagttccca aaagacgccc ggaacctgcc
      541 gaaacgcgcc cggcagcccc tggtccagtt atagacgagc cggcctcgcc ccgggttccg
      601 caacttgtgc tatcggagga taggcttcga ccgtgccaat cctttaaaat ggagggtatg
      661 agccgcatga agtttcgtgt ctgggcgccg ctcatggcgg tcgtcctggg tctggttttc
      721 gcagcccacg cgcaggacca tgtccgcggg cgaaaataca aagcgccccc gccgacctgc
      781 aaagtcaccg tgaccgtcat ccggggctac aacggaaaac ccctcgagaa cgcctcggtc
      841 gtcttccatc ccatcaagga tggcaaggat accggcggca tggaactgaa gaccgacgaa
      901 gacggcaagg tcaccctgga tatgatcccg gtcgacgaag acctgcgcct tcaggtgctc
      961 gccaccggct tccagacctt cggccacgac tacgatctca ccggcacctc caaagaaatc
     1021 gaagtccgca tgaaccggcc gcagaggcag tactccatct acgagagcca ccccggcaac
     1081 aacgccggcc aggccaccac cgagccgcag ggcgatcaga aagacaagga caaggacaaa
     1141 ccccagcagg accaaaagcc tcacgactag cttccgctgg gtgccccaca acctggcctg
     1201 aacgaagcct cacttcgtga ggcgagccga atggctccct gcggttgggg atgtgggtat
     1261 cgcgcagagc gcgacctcat tctgcctcaa ctcggcctat cgatagtcac ttcggccgtc
     1321 gcactacact ccggcttcct ccccccaacc cgctattctg aagtggccgt gtcctcctcc
     1381 cagccagcaa ccgcaagcct cccctccctg cacgatttct tcgcgccggg aggcgtgctg
     1441 tcgcggtccc cactccccta cgagttccgc cgcggccagc tcgagatggc ccaggccgtc
     1501 gagcgcgccc tccaggacca gcgacacctc atcgtcgagg ccggaaccgg aaccggaaag
     1561 accctcgcct atctccttcc cgcgctccgc cgctgcgccg ccaccggcca tcgcatcatc
     1621 atctccaccg gcacgaaaaa cctgcaggag cagctcttct tcaaggacat ccccttcctc
     1681 gaatcgctcc tcggcccgct gcgcgtctgc tacatgaagg gccgcgccaa ctacctgtgc
     1741 cgccacaagc tcttcgccct gcgcaaccag cccatccttt ccggtctgga agaaatctcc
     1801 cagtaccagg ccatcgccga ctgggaaaag accaccgaaa ccggcgatcg cgccgagatc
     1861 gacgacctgc ccgaagccag cgccctctgg tctaaactcg acgcgcgcag cgaggcctgc
     1921 ctcggccaga cctgtcccga ctacggccgc tgcttcatca ccgagatgcg ccgccgcgcc
     1981 gccgagagcg acgtcgtcat cgtcaatcac cacctcttct tcgcggacat ggcc
//
LOCUS       DASZYN010000583         3322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_478869, whole genome shotgun sequence.
ACCESSION   DASZYN010000583 DASZYN010000000
VERSION     DASZYN010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478869"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..619
                     /locus_tag="VHE33_15105"
     CDS             119..619
                     /locus_tag="VHE33_15105"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HVT98831.1"
                     /translation="MAAENSFDIVSKVDMQEVRNAIDQATKEVRTRFDLKDSKSDIHL
                     EGEDAIQLASVDEYKLQAVTEILRQKLVKRNVSLKSLTFGKVEPAAGSSVRQKITLQQ
                     GLAGEKAKEIVKIIKDSKKKVQASIQGDTVRVSGKDRDALQEVIAMLKGRDLGIDMQF
                     TNYRSN"
     gene            650..1642
                     /locus_tag="VHE33_15110"
     CDS             650..1642
                     /locus_tag="VHE33_15110"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HVT98832.1"
                     /translation="MSTVATSTAKEVFDLLHDDLAAIEREFSSDAVSSVSAITEISDY
                     LREGGGKRIRPSLLLLAARCLGYSGRGMIRLGAVVEMVHTATLVHDDIIDAADVRRGR
                     PSTNHTWGNGKCVLVGDWLYMQAFRIALEERNFRVLELLIGLTQQMVEGELLQMECLG
                     KPISAGEYYDLIYRKTACLFEVSMRLGAVLAGADQATEDSLGGYGRNLGLAFQIVDDV
                     LDLTATEEVLGKPVASDLREGKATLAVIHTLENGSTPAEQTAIQTVLEDQNFERVSHQ
                     EILRILNRNQSVNYAMKAAFDYAAAAQTALAAVPDSEYKRALQWMPEFVVAREK"
     gene            complement(2007..2480)
                     /locus_tag="VHE33_15115"
     CDS             complement(2007..2480)
                     /locus_tag="VHE33_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98833.1"
                     /translation="MTNVASSKIEAGLAAAIREFLSAEPNQFISEDENLREVCRWLAR
                     FTQELLNGKDDWGRYAWVDDISPCTAKGQPSGTFVLTALMIWVADDKKSPDWKEPLFA
                     EMHLSASSPPRLEYEIRVGDADCGLAKCPYSSLHEYPVVAVENWLFTFDSKHTNE"
     gene            2753..>3322
                     /locus_tag="VHE33_15120"
     CDS             2753..>3322
                     /locus_tag="VHE33_15120"
                     /inference="COORDINATES: protein motif:HMM:NF016494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HVT98834.1"
                     /translation="MKLSAIASALGVELRGPDSEIGAVASTASATPASLLFAEDAQTL
                     AAALASSAAAVIVSAKLAPATSEKALLIAAQPRLTFAQTARLLKPSEPATGIHSTAII
                     DPSVKLGEHLSIGPYVVIEGGTHIGTGTSIGAGSVIGQGVNIGLNCRIYPRVVLYPGT
                     ELGNNVVLHAGVVLGADGFGYVRDAETGEY"
BASE COUNT          679 a         1044 c          950 g          649 t
ORIGIN      
        1 ccggatgaag tcgtccacca caccacaagt aacttcaagg cgttttttcg taccgaaata
       61 ccggtctcaa ctcacgtttc cgggcagtaa aggataatct ggaagagaag aggcccccat
      121 ggcagcagaa aactccttcg acatcgtcag caaggtcgat atgcaggaag tgcggaacgc
      181 gatcgaccag gcgacgaaag aggtgcgcac gcgcttcgat ctgaaagatt cgaaatcgga
      241 tattcacctg gaaggcgaag acgcgatcca gctcgcctcc gtcgacgaat acaagttgca
      301 agccgtaacg gaaatcttgc gccagaagct ggtgaagcgg aacgtttcgc ttaaatctct
      361 taccttcggc aaagtggagc ctgccgccgg gtcctcggtg cgccagaaga tcaccctcca
      421 gcaggggctc gctggagaga aggcaaaaga gatcgttaaa atcattaaag actcaaagaa
      481 gaaggttcag gcttcgattc agggcgatac cgtccgcgta tcgggcaagg accgcgatgc
      541 tctccaggaa gtgatcgcga tgctgaaggg tcgcgatctc ggcatcgata tgcagttcac
      601 gaactaccgt tccaactgaa cggtcggccc cgcagtcgga gaaccttgag tgagcacagt
      661 ggcgacatcg acggcgaagg aagtgttcga cctgctgcac gatgaccttg ccgccatcga
      721 gcgggagttc agttccgatg ccgtatcctc ggtctccgcc attaccgaga tctccgacta
      781 cctgcgcgaa ggtggcggca aacgcattcg cccctccctg ctgttgctgg ccgcgcgttg
      841 ccttggctat tccggccgcg gaatgatccg cctcggcgcg gtcgtggaga tggtccatac
      901 cgccaccctg gtgcatgacg acatcatcga cgcagccgac gtgcgccgcg gccgcccctc
      961 caccaaccac acctggggca acggcaaatg cgttctggtt ggcgactggc tgtacatgca
     1021 ggccttccgc atcgcactgg aagagcgcaa cttccgcgtt ctcgaactgc tcatcgggct
     1081 cacccagcag atggtcgaag gcgaactgct gcagatggaa tgcctcggca agcccatctc
     1141 ggctggcgag tactacgacc tgatctaccg caagaccgcc tgcctgttcg aagtttccat
     1201 gcggctgggc gccgtcctgg ccggtgcgga tcaggcgacg gaggattccc tcggcgggta
     1261 tggccgtaac ctcggcctgg cattccagat tgtcgacgac gttctggacc tgacggcgac
     1321 cgaagaggtg ctgggcaaac ccgtggccag cgatctgcgc gaaggcaagg ccaccctcgc
     1381 cgtcatccac acccttgaga acggcagcac acccgctgaa cagactgcga tccaaaccgt
     1441 gctggaggat cagaacttcg agcgcgtgtc gcatcaggag atcctgcgca tcctgaatcg
     1501 aaaccagtcc gtgaactatg ccatgaaggc cgcgtttgac tacgcagcgg ccgcccagac
     1561 cgcccttgcc gccgttccgg actccgaata caaacgcgcc ctccagtgga tgccggaatt
     1621 cgtcgtcgcc cgcgaaaaat aatcgttcgc gagtcacatt cggtttctgg gtgaccgatt
     1681 ccaatcgtcc cctgttgctg gtttttcaac ctggtggcgt ttcctcgcga gcgaggagac
     1741 gccagggcgt gagggcgggc agtcggggtc ctggcgaaac accgtttcgc cgggatggct
     1801 gtgcaggtac caggagaacc ggtcacgtaa acccctggct tccaccttag ggtcagcttc
     1861 cggtttgccg ttacgccggc gctagtcttg ttgcgcattg ggtgctgcgg agcagcacga
     1921 cggtagaagc cccagccttc aggccgggga aatcagggat ctgatggcaa ggctttagcc
     1981 gcggctcgaa tgttccggat tgccagtcac tcgttcgtgt gcttcgagtc gaaggtgaac
     2041 agccagtttt caacagcaac aacgggatac tcgtggagtg agctgtacgg gcactttgcc
     2101 aatccgcaat cagcatcacc gactcgaatc tcatattcta ggcgcggggg ggaactcgct
     2161 gacagatgca tctcggcgaa gagtggttct ttccaatccg gggatttctt gtcgtccgcc
     2221 acccagatca tcaaggccgt cagcacgaac gttcctgaag gctgcccttt tgcggtgcac
     2281 ggtgagatat cgtcaaccca tgcatacctg ccccaatcgt ctttgccgtt gagcagttcc
     2341 tgagtgaagc gggctagcca cctgcaaact tcgcgaagat tctcatcttc ggaaatgaac
     2401 tgattaggct ccgcgctcag gaattcccgg atggcagccg ctagaccggc ctcaatcttg
     2461 ctggaagcga catttgtcat gcgtcagcat tttctcttat ttcccgagcg cgcggctctc
     2521 gaagtttctg tggtgtcggc aacccgaaag tttcatgcgg tggaagcaga aaagcaaacg
     2581 cagatccttc gctcgcctcg ccatctgcgt aaggcttcac gccgctcgca tccggacggc
     2641 gacgcaaagg tggtggggtg atgtctgggc aatccaccgt tcggaagttt cgtccgagac
     2701 ggagccgaaa acgcagtcct ttggggagtg ttttttcagt actctaatct ccgtgaagct
     2761 ttctgccatc gcctcggccc tcggcgtcga actccgcggc cccgattccg agatcggcgc
     2821 cgtcgccagc accgcgagcg caactcccgc ttccctgctc ttcgctgaag acgcccaaac
     2881 cctcgccgcc gccctcgcct cgagcgccgc cgctgtcatc gtttccgcga aactcgcgcc
     2941 cgcaaccagc gaaaaagcgc tcctcatcgc cgcccagccc cgcctcacct tcgcccaaac
     3001 cgcgcgcctg ctcaagccgt ccgagccggc caccggcatc cactccaccg ccatcatcga
     3061 cccttcagtc aaactcggcg agcatctctc catcggaccc tacgttgtca tcgaaggcgg
     3121 cacccacatc ggcacaggaa ccagcatcgg tgccggctcc gtcatcggcc agggcgtcaa
     3181 catcggcctg aactgccgca tctacccgcg tgtggtcctc tatcccggca ccgagctggg
     3241 caacaacgtg gtgctgcatg ccggggtggt tctgggtgcc gacggattcg gctatgtccg
     3301 cgacgcggag acgggcgaat ac
//
LOCUS       DASZYN010000584         1685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_159697, whole genome shotgun sequence.
ACCESSION   DASZYN010000584 DASZYN010000000
VERSION     DASZYN010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159697"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="VHE33_15125"
     CDS             complement(<1..1106)
                     /locus_tag="VHE33_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98835.1"
                     /translation="MSRIARLILGLLAGLALLTWAASGVVETTAREWFERDVSARAHL
                     FLTGANRSLAQAWNNPMELHGDLVAIARDEHVMATAACGSDLSTRAETAAFPGEFNCL
                     EVGPRMLIDARPGGAEQPTFREWSTIATLPTGRVHVSAMPVRDDSQQLGFVILVQDLG
                     YIQKREDRARSFLIAISLLLAITAFGVPLVAARRARHDWSLELRRLLRGGGRTRREFQ
                     PILSDLRELVGRMSNEQHENSGQWTAASLKQTLNRHLHGERIVILANREPYIHERMPD
                     GGIDVQHPASGLVTALEPVMRACSGVWVAHGSGSADRDTVDREDRVGVPPGEEVYRLR
                     RVWLSEEEQQGYYYGFSNEGLWPLCHVADTRPVFR"
     gene            1297..>1685
                     /locus_tag="VHE33_15130"
     CDS             1297..>1685
                     /locus_tag="VHE33_15130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVT98836.1"
                     /translation="MAPLLTPREAAMRLGISYAAMKQWLYRGKLKAIKTPGGHYRIPE
                     SELDALLFKAKRPETPKRQMMRTVSGRNQLVGRIVDLKIEGLLAQVRLSIGGQIITSI
                     ITADAAREMRLKKGETVAALIKSTEVMV"
BASE COUNT          329 a          612 c          433 g          311 t
ORIGIN      
        1 cggaaaaccg gtcgcgtgtc cgctacgtgg cacagcggcc acagtccttc attcgagaag
       61 ccatagtagt acccctgctg ctcctcctcg gacagccaca cacgccgcag ccggtagacc
      121 tcttcccctg gcgggacccc gacacgatct tcgcggtcaa ccgtgtcccg atctgcgctc
      181 ccgctcccat gtgccaccca cacgcccgaa cacgcacgca ttaccggctc cagcgctgtc
      241 accaggccgc tcgccggatg ctgcacgtca atcccgccgt ccggcatgcg ctcgtgaata
      301 tagggctccc ggtttgccag gatcacaatc cgctcgccat gcaaatgccg gttcagcgtt
      361 tgtttcaggc tcgccgccgt ccactgaccc gaattctcgt gctgctcgtt cgacatccgg
      421 cccaccagct cgcgcagatc gctcaggatc ggctgaaact cgcgccgcgt ccgccccccg
      481 ccgcgcagca gccggcgcag ctcgaggctc cagtcatgtc gagcacggcg agctgccacc
      541 agcggtaccc cgaatgccgt aatcgccagc agcagcgaaa tagcaatcaa aaagctgcgc
      601 gcccgatctt ctcgcttctg gatatatccc aggtcctgca ccagaatcac aaatcccagc
      661 tgctggctgt cgtcccgcac cggcatcgcg ctcacgtgaa cgcgtcccgt cggcagcgtg
      721 gcaatcgtgc tccattcccg gaacgttggt tgctccgctc ctcccggacg cgcgtcgatc
      781 agcatgcgcg gtcccacttc aaggcagttg aactctccag gaaacgctgc cgtctccgcc
      841 cgcgtgctca gatccgagcc gcaggcggcg gtggccatca cgtgctcatc gcgcgcaatg
      901 gccaccagat ccccatgcaa ttccatgggg ttgttccacg cttgggccag cgagcggttt
      961 gccccggtca gaaacagatg cgcgcgcgcg ctcacatcgc gctcaaacca ctcccgcgcc
     1021 gtggtctcca ccacgcctga ggccgcccag gtcagcaaag ccagccccgc cagcagcccg
     1081 agaatcaacc gcgctattcg cgacatatgc aatctaaggc tacaactccg gaagcctctg
     1141 cgctccttct ccactgcatc cgctgtctta ccttgccggt ggattttcca cttcgttcat
     1201 ggtgcgatgc cgattccgga caggcaggtt caacaatggc tcaatatcgt cggatatcac
     1261 ataagtaaag cgctcatttt cgactaaaat cgttgcatgg cgcccctcct gactccgcgc
     1321 gaagcggcca tgcggcttgg catctcgtac gctgccatga agcagtggct ttatcgcggg
     1381 aagctcaagg cgatcaaaac tcccggcggt cactaccgca tccctgaatc ggagctcgac
     1441 gcccttctct tcaaagcaaa acgtccggaa actccaaaac gtcagatgat gcgcaccgtc
     1501 agcggccgca atcagcttgt cggacgcatc gtggatttga agatcgaagg cctccttgcg
     1561 caagtccgcc tctccatcgg cggccagatc atcacctcca tcatcaccgc cgacgctgcc
     1621 cgcgagatgc gcctcaaaaa aggcgagacc gtcgccgccc tgatcaaatc caccgaagtg
     1681 atggt
//
LOCUS       DASZYN010000585         3555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_405649, whole genome shotgun sequence.
ACCESSION   DASZYN010000585 DASZYN010000000
VERSION     DASZYN010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405649"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VHE33_15135"
     CDS             <1..367
                     /locus_tag="VHE33_15135"
                     /inference="COORDINATES: protein motif:HMM:NF012462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0042255 - ribosome assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uL22 family ribosomal protein"
                     /protein_id="HVT98837.1"
                     /translation="SALQNANHLSQEQGLDVDVDSLYVKSAVANDGPRMKRIRPAPMG
                     RAYRYQRRLSHIQIALAERGHAEGLVHRVEDDSAAAPVKAAAKKTAGSKPAKKAAPKK
                     AAAHKPAKKAAAKKPAKKK"
     gene            443..1114
                     /gene="rpsC"
                     /locus_tag="VHE33_15140"
     CDS             443..1114
                     /gene="rpsC"
                     /locus_tag="VHE33_15140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HVT98838.1"
                     /translation="MGQKVHPYGFRVGVNKPWRSRWFANRDYSRLLVEDVHLKRELKD
                     KLKAAGVSSVEVERPGNKLRLMIRTARPGIVIGRKGAEIEKLKGELQKRTNRDVFIDI
                     LEVNKPELDAQLVAENIALQLEKRVGFRRAMRKAVDSALRFGCKGIKVRVSGRLNGNE
                     IARSEWYLQGRLPLHTLRADIDYGFAEARTTYGVIGVKTWIYRGDIYQQKRREPQTAV
                     GTSVF"
     gene            1229..1657
                     /gene="rplP"
                     /locus_tag="VHE33_15145"
     CDS             1229..1657
                     /gene="rplP"
                     /locus_tag="VHE33_15145"
                     /inference="COORDINATES: protein motif:HMM:NF012476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HVT98839.1"
                     /translation="MPKKVKYRKQQRGRMRGKAWRGSELSFGDFGLKVMECGYITDRQ
                     IEASRVAMTRFIKRGGKIWLRLFPDKPVTKKPAETRMGKGKGAPDHWVAVVRPGRILF
                     EMEGVTPDLAKEAMRLAAHKLPLKTKFVQRHDVKVVAAAK"
     gene            1764..2186
                     /gene="rpmC"
                     /locus_tag="VHE33_15150"
     CDS             1764..2186
                     /gene="rpmC"
                     /locus_tag="VHE33_15150"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HVT98840.1"
                     /translation="MEVEKIRNLSDEELALEGRKASEALFRLRFQMKLGQTEGAKKLR
                     ELKKDIARINTIGRERELGIHTLVAPKHHHAHAEGQAEGAAPVKSAKKKTGKSAKKKT
                     AKKSTRKAGGRSAAARKTTAKPSANKKKAARSSSVKKG"
     gene            2191..2529
                     /gene="rpsQ"
                     /locus_tag="VHE33_15155"
     CDS             2191..2529
                     /gene="rpsQ"
                     /locus_tag="VHE33_15155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HVT98841.1"
                     /translation="MSATEQTKTEAAQGKRNEKVGQVVSTKMQKTIVVEVEMRKAHPK
                     YKRIVKSNRKFYAHDEQNTARVGDVVRIRETRPLSKLKRWNLEEIVRRSALAQVQEAE
                     AAAGISADPQ"
     gene            2594..2965
                     /gene="rplN"
                     /locus_tag="VHE33_15160"
     CDS             2594..2965
                     /gene="rplN"
                     /locus_tag="VHE33_15160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HVT98842.1"
                     /translation="MAVQMRTMLEVADNSGARKLQMILPLGGASGLKAGLGDVITAAV
                     KEASPDGQVKKGKVVKAVVVRTRKETRRRDGTYIRFDTNAAVLINDAGEPVGTRVFGP
                     VARELREKKFLKIVSLAPEVL"
     gene            2994..3326
                     /gene="rplX"
                     /locus_tag="VHE33_15165"
     CDS             2994..3326
                     /gene="rplX"
                     /locus_tag="VHE33_15165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HVT98843.1"
                     /translation="MASLDIQRNDTVQVISGSDRDKKGRVLRVFPDTGRLLVEHVRVV
                     KKHLRENPQRRIKGGIAEQEAPILRSNVMLVCPNCNQPTRVGHKFEGDTKVRVCKKCG
                     GTIAVKKK"
BASE COUNT          850 a          953 c         1221 g          531 t
ORIGIN      
        1 ctcggcgttg cagaacgcaa accacctgag ccaggagcag gggctcgatg tggatgtgga
       61 cagcctctac gtgaagtcgg ccgtggccaa tgacggtccg cgcatgaagc gcatccggcc
      121 ggctcccatg gggcgcgctt atcggtatca gcggcgtctg tcgcatatac agattgcgct
      181 ggctgaacgc gggcacgccg agggcctggt gcatcgggta gaagacgatt cggcggcggc
      241 gcccgtgaag gccgcggcaa agaaaacagc cggaagcaag ccggcgaaaa aggcggcgcc
      301 gaagaaagcg gccgcgcaca aaccggccaa aaaagccgcg gcgaagaagc ccgcgaagaa
      361 gaaataggac ttaccgtccc ggcggatgcg gctttgccgc agaagccagg acgggaagcg
      421 agcgagagag gattcagaga ttatgggaca gaaagtccac ccttacggat tccgggtcgg
      481 cgtcaacaag ccctggcggt cgcgctggtt cgccaaccgc gattacagca ggctgctggt
      541 tgaagacgtt catctgaagc gcgagctgaa ggacaagctg aaggcggccg gcgtcagctc
      601 ggtcgaagtc gagcggccag gcaacaagct gcgcctcatg attcgcaccg cgcgtcccgg
      661 catcgtgatc ggccgcaagg gcgccgaaat cgagaagctg aagggcgagc tgcagaagcg
      721 caccaaccgc gatgtgttca tcgacattct cgaggtgaac aagccggagc tggacgcgca
      781 gctggtcgcg gagaacatcg cgctgcagct ggaaaagcgc gtcggcttcc gtcgcgcgat
      841 gcgcaaggcc gtcgactctg ctctccgctt cggttgcaaa ggcatcaagg tgcgtgtttc
      901 gggccggctg aacggcaacg aaatcgcccg ctcggagtgg tatctgcagg gccgtctgcc
      961 gctgcacacc ctgcgcgccg acatcgacta cggatttgcc gaagcacgca cgacctatgg
     1021 cgtgatcggc gtcaagacgt ggatctaccg cggagatatc tatcagcaga agcggcgtga
     1081 gcctcagacc gcggtgggca cgagcgtttt ttaaggagct gccggcgcct ggctcttagc
     1141 gggttggctg gcaggattgg ttgttgtggt ttgagctggc agcaggaagc tgagagctgg
     1201 aagctgtttt tgagggtttt ctatgttgat gccaaagaaa gtgaagtacc gcaagcagca
     1261 gcgcgggcgc atgcgcggca aggcgtggcg cggcagcgag ctgtcgttcg gcgatttcgg
     1321 tctcaaggtc atggaatgcg gttacatcac cgaccgtcag attgaagcca gccgtgtcgc
     1381 catgacgcgc ttcatcaagc gcggcggcaa gatctggctg cggctgtttc ccgacaagcc
     1441 ggtgaccaag aagccggccg aaacccgtat gggcaaaggc aagggcgccc ccgatcactg
     1501 ggtagccgtg gttcgccccg ggcggatcct gttcgagatg gaaggcgtca cgcccgatct
     1561 ggccaaagaa gccatgcggc tggcggccca caagctgccg ctgaagacca aatttgtgca
     1621 gcggcacgat gtcaaggtgg ttgccgcggc gaagtagctg gagatgttaa tgggagtggt
     1681 ggttggcagg actggaacgc ttagcagatt ttgaaatacg ggtgggatcc tggtactggg
     1741 tccagccgga gagaagagca acgatggaag tagagaagat tcggaacctg agcgacgagg
     1801 agctggcgct cgaagggcgc aaggcgtcgg aggcactgtt ccggctgcgg tttcagatga
     1861 agctcggcca gaccgagggc gcaaagaagc tgcgcgagct caaaaaggac atcgcgcgga
     1921 tcaacaccat cgggcgcgag cgcgagctgg gcatccacac cctggtagcg ccgaaacatc
     1981 atcacgcgca tgccgaaggg caagccgaag gcgccgcacc ggtgaagagc gcgaagaaga
     2041 agaccggaaa atcggcgaag aagaagaccg cgaagaaatc cacgcgcaag gccggcggac
     2101 gctcggcggc ggcgcggaag acgacggcga agccctcggc aaacaagaaa aaggctgcgc
     2161 ggtccagcag cgtgaagaag ggttaacgag atgagcgcaa cagaacagac taaaaccgaa
     2221 gccgcgcagg gcaagcgcaa cgagaaggtc ggccaggtcg tctccacgaa aatgcagaag
     2281 accatcgtgg tcgaagtgga gatgcgcaag gcccatccca agtacaagcg catcgtcaag
     2341 agcaaccgca agttctacgc gcacgacgag cagaacacgg cgcgggtggg cgacgtggtc
     2401 cgcatccggg aaacccgccc gctgagcaag ctgaagcggt ggaacctcga agagatcgtt
     2461 cgccgttcgg ccctggccca ggtgcaggaa gccgaagcag cggcgggaat cagcgccgac
     2521 ccgcagtaac tgcgttgtaa gtcgagatcg cggcgagtga gatcgaccgc gacagcagga
     2581 aggagagaat gccatggcag tgcagatgag aacgatgctc gaggtcgccg ataacagcgg
     2641 cgcgcgcaag ctgcagatga ttctgcctct gggcggcgcc agcggactca aggcgggcct
     2701 cggtgacgtg atcaccgccg ctgtgaagga agcttcgccg gacggacagg tgaagaaggg
     2761 caaggtggtc aaggcggtcg tggtgcgcac gcgcaaggaa acacggcggc gcgatggaac
     2821 ctatatccgc ttcgacacta acgccgcggt tctgatcaac gatgcgggtg agccggtcgg
     2881 cacccgcgtg ttcggtccgg tggcgcgcga actgcgcgag aagaagtttt tgaagattgt
     2941 gagcctggct ccggaagttc tctgaagaga tcaaggatga aggaacaaag attatggcga
     3001 gtttggatat tcagcgcaac gatacggtgc aggtgatctc gggcagcgat cgcgataaga
     3061 agggacgcgt gcttcgcgtg tttcccgata ccggtcgctt gctggtcgag cacgtgcggg
     3121 tagtgaaaaa gcacctgcgg gagaacccgc agcgcaggat caaaggcggc atcgccgagc
     3181 aggaagcgcc gattctccgc tcgaatgtga tgctggtttg ccccaactgc aaccagccca
     3241 cgcgcgtagg acacaagttc gaaggcgaca ccaaggtgcg ggtctgtaag aaatgcggcg
     3301 gaaccattgc cgtgaaaaag aagtaggact gggccgtcca ggcgtgcgcg ccttcggcac
     3361 agaagttcag aaaggaaaac attcgtaacg gtagacggct tcagtgccaa tccgagaccg
     3421 aatgagaatt gagcagagaa atggcagcga gactgaaaga gaagtaccac aaggaaatca
     3481 agggcgctct gcagaaggaa ctgggcctcg agaaccccat ggccgtgccg cgcctggaaa
     3541 agatcgtcct caaca
//
LOCUS       DASZYN010000586         6450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1054633, whole genome shotgun sequence.
ACCESSION   DASZYN010000586 DASZYN010000000
VERSION     DASZYN010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054633"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            893..1960
                     /locus_tag="VHE33_15170"
     CDS             893..1960
                     /locus_tag="VHE33_15170"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVT98844.1"
                     /translation="MISLKKYLDQKDQRPHPGQTADETVQCYRSLLLAVGRSAVQGSP
                     AVGADLERSLKGLEHRLSIHSSASALLHTEKQVEVQLQEWGSRTAEHFKTKADEVREL
                     LIALAKTAEAVGDRNQGYSTQFRNLTSRLETIADLDDLTQIRTSVVASVNDLKSQVEK
                     MTRDNQELVENLRSEVTTYENRLKTVENLILKDEITGVASRRSVEERMQRCIDNQMPF
                     CVIMIDLNGFKHVNDKYGHLAGDDLLRQFAMELQMYTRSGDMVGRWGGDEFVVLLSSD
                     AAGAASHMDRIREWVFGKYTIQRGANKDATVLHMDASLGMAEWKDNKTMQEVIAEADM
                     AMYRDKKKSHAKPGIQEPVLT"
     gene            complement(2408..3529)
                     /gene="prfB"
                     /locus_tag="VHE33_15175"
     CDS             complement(2408..3529)
                     /gene="prfB"
                     /locus_tag="VHE33_15175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HVT98845.1"
                     /translation="MNSLTLPSAKKCAVCGSIFDSPRLKTELAAIETKLADPALWSNP
                     AQSQPLMRDRKRIEAQLADDAELERRTGDVDAYFELAREGETVEPDLEREIKALGDLV
                     EQLEFRTMLSGETDPLNAIITVHPGAGGTESQDWAEMLMRMYLRWAEQQGFRTEMNDY
                     QDGEEAGIKSATFTITGEYAFGLLSGETGVHRLVRISPFDQAKRRHTSFASVFVSPEI
                     DDSIQIDIKPDEIRTDTYRSGGKGGQHVNTTDSAVRITHIPTNIVVQCQNERSQHKNR
                     EKAMKMLRSRLYEYELEKKQAATKKLEDSKLDINFGSQIRSYVLQPYRMAKDHRTKVE
                     VGDVDKVLDGFLDPFIRGYLLMKRQGGVPVAAAAEDDLD"
     gene            complement(3545..5131)
                     /gene="lnt"
                     /locus_tag="VHE33_15180"
     CDS             complement(3545..5131)
                     /gene="lnt"
                     /locus_tag="VHE33_15180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HVT98846.1"
                     /translation="MGLGTAVLLDFCFPVAGPMPRWHSLIAWVALIPLLWALLGERAV
                     QHPRYLRRAALTGYVCGVLWYILNCYWIYQTMLYYGHVPPLGSAGIVLLFSAVLGLYF
                     GAFGLGMALVRRRFGFGWALAIAPFLWTALELAAARITSVPWDQLGYSQVDHQTLTRL
                     APWTGVYGISFVLVAGNALLLADWIPVKRVHPSLRFVIRLVVLALVWLSLGLRDPAPS
                     PTSDYAVLLQPNIDVEPPDNWKGPQWEGQVNWLVDAARQTCTPAYGGMPLEKMAPPSR
                     TCAQNAPPPGVVVWPEVGSWFQSDDPRTLALVRTVATSADAPFIAGMFGHDVNGTYNS
                     ALFANSDGTIGGRYDKIHLVPFGEFVPYRDLFFFAKKLTHQLVDLQRGRERRVFVADG
                     HTFGVFICYESVFADEVRLFAKNGAQVLVNISDDGWYGDTSAPWQHLNMARMRAIEND
                     RWLLRDTNNGVTTAIDPYGRVTVSAPRHTITSLVARYGYTSDLTFYTKYGDVFAYACC
                     AVAIAALLFSLGGPARIEST"
     gene            complement(5222..5350)
                     /locus_tag="VHE33_15185"
     CDS             complement(5222..5350)
                     /locus_tag="VHE33_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98847.1"
                     /translation="MIAIGLAAMHVLNWMFVVGFIGSLLVVVISFFEDLGAILGKE"
     gene            5566..>6450
                     /locus_tag="VHE33_15190"
     CDS             5566..>6450
                     /locus_tag="VHE33_15190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HVT98848.1"
                     /translation="MTQQISITVVGSGYVGLVATACFAEIGHRVICVDNDEAKLRTLA
                     AGGVPIHEEYLPELLDRHRGHSIEFTSDLRAATQSAQAVFIAVGTPQSRTGSADLSYV
                     DAVASEIARSIDEYKVIIEKSTVPVYTNEWVRRVIERNGVARELFDVASNPEFLREGT
                     AVVDFLHADRIVIGTDSDRAADLLKRIYSPLTSGAYYGSDGAVPGCCNDARRPPLLQT
                     STKAAEIIKHASNAFLATKISFINVVANICEAAGADIEQVARGIGMDSRIGPKFLRAG
                     IGYGGSCFPKDVAAFRYVA"
BASE COUNT         1409 a         2076 c         1770 g         1195 t
ORIGIN      
        1 ttctgaatat agggagagag aaactctttc tgccctcccg atcagcttca gcagcaccca
       61 aaaggctcgc cataagcgag ccttttctct tgcagacaga aggtcaacga tgcccaagcc
      121 gccgtcaccc atggttcgcg accgtgagtc acgtacggcg cgggcaagcc tcgtccaaag
      181 ttgtcaaact tgtaaacatc gccacacccc tcaaaacaaa agtcttacaa cttgtccctc
      241 ttcgaaaaca caccatcgtc ttgcgaggcg acccgcaggt agcaaatcga ttccgacgaa
      301 tcctccggag cgagtctcgt cctccccgtt cgcaaccccg actccggaac gccgaacgcg
      361 ccccacggaa cacctctccg ctccacccgc cttcgcgctc tcgaatgatc caatgacaca
      421 cgcgaatgaa tcagttgcgt gcgctatccc atccatcacg cgcgccactg catcaatccc
      481 acgcgcctcc catctgactt cttcccaatc atctggcttt ctcgcgactg cgagtgacgc
      541 atgcacagtt gcgaatgcat cattcacacg cgtcagcgat ctgactcgcc ctcagtcact
      601 tgtctgttat ccactttgtc accaaagtcc aactcccgca gaatcaatca gcctgaattc
      661 cgccgaggac cgggacccat gtcatcccgc aatcagctcc gcctacgact ttcgtggcct
      721 tgctccccaa aatcgctgga attccagccc caatatgccg aagagtcgcc atcggcacag
      781 atgtcaggcc ttcgctcgcc cgactgccga gaaaagaaca agacgtcccg acgtgggccc
      841 gaatgcaatc ctccagccca ggcccgggac gcgaaacccg ggagaacgaa cgttgatttc
      901 ccttaagaaa tacctcgacc agaaagacca gcggccccac ccaggccaga ccgcggacga
      961 aaccgtccag tgctatcgca gccttctcct cgctgtcggc cgcagcgccg tgcagggaag
     1021 ccccgctgtc ggcgccgacc tcgaacgctc gctcaagggc ctcgagcatc gcctctccat
     1081 ccactcttcc gcctctgctc ttcttcacac cgagaagcag gttgaggtgc agctccagga
     1141 atggggatcc cgcaccgctg agcactttaa aaccaaagcc gacgaggtta gggaacttct
     1201 catcgctttg gcgaaaaccg ccgaggccgt cggcgatcgc aatcaaggct attccaccca
     1261 gttccgcaat ctcaccagcc ggcttgaaac catcgctgac ctggacgacc tcacccagat
     1321 ccgcacctct gtcgtcgcca gcgtcaacga tctgaagagt caggtcgaga aaatgacgcg
     1381 cgacaaccag gagctggtcg agaacctccg ctccgaagtc acgacctatg agaatcgcct
     1441 caagaccgtc gaaaatctca ttctgaaaga cgagatcacc ggagtcgcca gccgccgcag
     1501 tgtcgaggag cgcatgcaac gctgcatcga caaccagatg cccttctgcg tcatcatgat
     1561 cgacctcaac ggattcaagc acgtcaacga caaatatggg cacctcgccg gcgacgacct
     1621 ccttcgtcag ttcgccatgg agctgcagat gtacacccgc tccggcgata tggtcggccg
     1681 ctggggtggc gacgagtttg tcgtcctgct ctcctccgat gccgccggag ccgccagcca
     1741 catggaccgc atccgcgaat gggtcttcgg caaatacacc atccagcgcg gagccaacaa
     1801 agatgccacc gtgctccaca tggacgcctc cctcggcatg gccgaatgga aggacaacaa
     1861 gaccatgcag gaggtcatcg ccgaggccga catggccatg tatcgcgaca agaaaaagag
     1921 ccacgcgaaa cccgggattc aggagcccgt gctgacgtaa cggatcggtg gatcctcagc
     1981 gtaaggctgc atcccggatc gcgtatcaag tcgcgttaac ggaggctttt gtacgctgga
     2041 acagaggctt ttgtgcgtta ggccgcaggc tttgtgcgtt agaaccgagg cttttgtacg
     2101 ccggaacaga ggcttttgta cgttaggccg caggcttcgt gcgttagaac ggaggctttt
     2161 gtacgccgga accgaggctt ttgtgcgtta ggccgcagac tttgtgcgct agaacggagg
     2221 cttttgtacg ccgcaacaga ggcttttgtg cgttaggccg cagactttgt gcgctggaac
     2281 ggaggctttt gtaacagggc acgaccttgg tcgtgccgtc cagcgcgcca aatgaacgcg
     2341 gctttagccg ctgaggcatt ttcaggattt tcggaacgag ttttgcgcgc ggcggccgtg
     2401 ggctggacta atccagatcg tcctcggccg ctgccgcaac gggaaccccg ccctgccgct
     2461 tcatcagcag gtatccgcga atgaatggat ccagaaatcc gtcgagcacc ttatccacgt
     2521 cgcctacctc aaccttcgtc cggtgatcct tcgccatgcg atagggctgc agcacatagg
     2581 accggatctg tgagccgaag ttgatgtcca gcttcgagtc ctcgagcttc ttcgtcgccg
     2641 cctgcttctt ctccagttcg tactcgtaga gacgcgaccg cagcatcttc atcgccttct
     2701 cgcgattctt gtgctgcgag cgctcgttct ggcactgcac cactatgttc gtcggaatgt
     2761 gtgtgatacg caccgccgag tcggtcgtgt tcacgtgctg cccacccttg cctcccgacc
     2821 gatacgtatc ggtgcgaatc tcgtcgggct tgatgtcgat ctggatcgag tcgtcgatct
     2881 ccggcgacac aaacaccgac gcaaacgacg tgtgccgccg cttcgcctga tcgaacggcg
     2941 agatgcgcac caggcgatgg acacccgtct cgccggaaag gagcccgaac gcatattctc
     3001 ccgtgatcgt gaacgtcgcc gacttgatgc ccgcctcttc gccgtcctga taatcgttca
     3061 tctccgtcct gaagccctgc tgctcggccc agcgcaggta catgcgcatc agcatctccg
     3121 cccagtcctg cgactccgtg ccgcccgcac ccggatgcac ggtgatgatc gcgttgagcg
     3181 gatccgtttc acccgacagc atggtacgaa actcgagctg ctcgaccaga tctccgagcg
     3241 ctttgatctc tcgctcgaga tcgggctcca cggtctcgcc ttcgcgcgcc agttcgaagt
     3301 aggcatccac atcgcccgtg cgccgctcca gctcggcgtc gtcggccagc tgcgcttcga
     3361 tgcgtttccg gtcgcgcatc agaggctgcg actgcgcagg gttcgaccac agcgccggat
     3421 cggccagctt cgtttcgatg gcagctagtt cagttttaag ccggggcgag tcaaagatac
     3481 tcccgcagac cgcgcacttt ttcgcggacg ggagcgtaag cgaattcaag atcggagagc
     3541 atgtctaagt cgattctatc cgagcagggc cgccgagcga gaagagcagt gctgcgatcg
     3601 ccacggcgca acaggcgtag gcgaatacat ccccatactt cgtgtagaac gtcagatcgg
     3661 atgtgtagcc ataacgcgcc accagcgacg tgatcgtgtg ccgcggcgcc gacaccgtca
     3721 cccgcccgta gggatcgatg gccgtcgtta caccgttgtt cgtgtcgcgc agcagccagc
     3781 ggtcgttctc aatggcccgc atccgcgcca tattcaggtg ctgccaggga gcgctcgtat
     3841 cgccatacca gccatcgtcg gaaatgttca ccagcacctg agcgccgttc ttcgcaaaca
     3901 agcgcacctc gtcggcaaag accgattcgt agcagatgaa gacgccaaac gtatgcccgt
     3961 ccgccacaaa cacgcggcgc tcacgccccc gctgcagatc caccagctga tgcgtcagct
     4021 tcttcgcaaa gaagaacaga tcgcggtacg gtacaaattc gccaaagggc acgaggtgaa
     4081 tcttgtcgta gcgcccgccg atcgtgccat cagaattggc aaagagagca gagttatagg
     4141 ttccgtttac atcatggcca aacatgccgg caatgaaagg cgcatcggca gacgtcgcaa
     4201 cagttcgaac cagcgccaga gtgcgaggat cgtcgctttg aaaccacgaa ccaacctccg
     4261 gccagaccac gacgccaggc ggtggcgcgt tctgggcaca ggtcctgctc ggcggcgcca
     4321 tcttttccag cggcatcccg ccatatgccg gtgtgcaggt ctggcgcgct gcatccacca
     4381 gccagttgac ctggccctcc cactgcggac ccttccagtt atccggaggc tcgacgtcaa
     4441 tgttcggctg cagcagcacc gcatagtccg aagtgggcga aggagccgga tcgcgaaggc
     4501 cgaggctcag ccacaccagc gcgagcacaa cgagccggat gacgaaacgc agcgaaggat
     4561 gcacgcgctt gaccggaatc cagtccgcca gcaacaacgc gttgcccgcg acgagcacaa
     4621 aggagatccc gtagacccca gtccacggag cgagccgcgt cagcgtctga tgatcgacct
     4681 gcgaataacc cagctgatcc cagggaacgc tggtgatccg cgccgcggcc aattccagcg
     4741 cagtccacag aaacggcgca atcgccaatg cccagccgaa tccaaagcgt cggcgaacca
     4801 acgccatccc aagcccaaaa gcgccgaagt acaaacccag taccgcactg aacaggagga
     4861 caatacccgc cgatccgagc ggcggcacat gcccgtagta gagcatcgtc tgatagatcc
     4921 agtagcagtt caggatgtac cagagcaccc cgcacacata tccggtcagc gccgcccggc
     4981 gaagatagcg gggatgctgg accgctcgct caccgagcag ggcccagagc aaaggaatga
     5041 gagcgaccca ggcgatcagc gaatgccacc ggggcatcgg gccggcaacg ggaaaacaaa
     5101 agtccagcag gaccgctgtg ccgagtccca taaaaatgga acccgtcgga gcaaaacgtc
     5161 cggaaccaga ggttttcgta acagaggtct tagcgagggc agccacgcgc cggttccgag
     5221 gctattcctt acccagaatt gcgcccaggt cttcaaagaa gctgataacg acgaccagca
     5281 aagacccaat gaacccgacg acgaacatcc agttcagaac gtgcattgcg gcgaggccaa
     5341 ttgcgatcac agcagcattc taaatcaccg gcctgccggt ttggtcccca aaccgaattc
     5401 acctgctggt tattgagacc tgccagcggc tgcgcattgg agcgggaatc gcagcataca
     5461 gcatggtagg atgaggcgag ttaacgcctg aaaatgggcc atggagctcg cccgcatgtc
     5521 cggcttccgc caaatcacca ggacacgagc gatgattgaa cggggatgac tcagcaaatc
     5581 tccatcaccg ttgtgggttc gggctatgtc ggtctggtcg cgactgcctg cttcgcggaa
     5641 atcggtcatc gggtgatttg cgtcgacaat gacgaagcaa agctgcgcac tcttgctgcc
     5701 ggcggtgtcc ccatccatga ggagtatctg cccgagttgc tggatcgcca tcgcggccac
     5761 tccatcgagt tcaccagcga tctgcgggcc gcgacccagt ccgcacaggc cgtctttatc
     5821 gcagtcggaa caccccagag ccgcacaggc agcgccgatc tgtcctatgt cgacgcggtt
     5881 gccagcgaga tcgcccgctc cattgacgaa tacaaggtga tcatcgaaaa gagcaccgtg
     5941 ccggtttaca cgaacgaatg ggtacggcga gtgattgagc gaaacggagt cgcgcgcgaa
     6001 ctgttcgatg tcgcgtccaa tccggaattt ctccgtgagg gaacggctgt tgtggatttt
     6061 ctgcacgccg accgcattgt catcggtacg gactccgatc gagccgccga cctcctgaag
     6121 aggatctata gtcctctcac atcgggggcc tactatggct cagatggcgc agtgcccggt
     6181 tgctgcaacg acgccaggcg gccgccgctg ctgcagactt ccaccaaggc ggccgagatt
     6241 atcaaacatg cctcgaacgc ctttctggcg acaaaaatct cgttcatcaa cgtcgtggcc
     6301 aatatctgtg aggccgccgg tgctgatatc gaacaggtcg ctcgcggtat cggcatggat
     6361 agccgcatcg gaccgaagtt tcttcgtgcc ggcataggct acggtggctc ctgcttccca
     6421 aaggatgtcg cggctttcag atatgtcgcc
//
LOCUS       DASZYN010000587         5544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_479362, whole genome shotgun sequence.
ACCESSION   DASZYN010000587 DASZYN010000000
VERSION     DASZYN010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479362"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VHE33_15195"
     CDS             complement(<1..923)
                     /locus_tag="VHE33_15195"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVT98849.1"
                     /translation="MCSFGVVACVIITCCCLLLPSRAAFAQVDEGSISGTVVDSTGAV
                     VPNAQVTLLNTDQGITVQTSTAAGGTYTFAPVRAGHYEITVTAQGFSKTTQSNLTVNV
                     GTPLTVNIQLKVGQASETVQVTEAPPLLQTEEASTGQVIGRESVNNLPLNGRNFTFLA
                     QLGAGMQSPQADTRGNAASGAFSANGERPAQNNYLLDGIDNNSDNVDFLNGTNYVILP
                     PIDAIQEFKVQTGDYSAQLGRAAGAVLNATVKSGTNSFHGDVWEFFRNDKLDAADWFE
                     NNKANPVKGEYRFNQFGATAGGPILRNKFFF"
     gene            1294..5067
                     /locus_tag="VHE33_15200"
     CDS             1294..5067
                     /locus_tag="VHE33_15200"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HVT98850.1"
                     /translation="MGLAPLLLRPSPITGFGRLWLPGMPDWAAGLSSAEVQLHPHYPQ
                     ESPLAAVMRLVEPGLDGYLTEKYAAQIEAVLDNWGRALCGSGDSRAVLAAFASPAIEA
                     SDFGQKKERPLRSAFGVTVLRRDFVQSVTQGRDAFLSAIAVWLGEIRRVETAEFEIYG
                     IETIAVSPLVVKAEIRYDLVALRADGRREERVGSVQTEWRSEEQGWMAQRWLLQEETV
                     SIVEQPAFVDVTAHALGETESWKAQMLFGADYWRTVLDGAVGLDVYSNNGVAAGDFDG
                     DGFDDLYVCQPSGLPNRLYRNRGDGTFEDVTARAGVAVLDNTACALFADFDNRGLQDL
                     LVVCGTGPLLFMNQGNGTFALKRDAFRFAGAAEGTFTHAAAADYDGDGRLDLYLCTYQ
                     YYLGLDQYHYPVPYYDARNGPPNCLFHNEGGHVFVEATEKAGLNIQNNRYSFASAWGQ
                     AGGGGKPDLFVANDFGSSQLYRNNGNGTFTDASEAAHVEGVGAGMSCCWHDFDNDGRQ
                     DIYVPSMWEAAGQRVTEQKQFHPDAPERIRALYRRHARGNALYRNRGDGVFENVAQTA
                     GVEMGRWSWSSDFWDFDHDGLPDLYVTNGYVSGADRADLASFFWRQVVAHSPEDASAS
                     TSYEQAWNAINELIRSDRTWHGYARNVMFANNGDGTFAEISGPAGLDFAQDCRSFALA
                     DLDHDGRLEVVLKSRNAPQLRILRNAMPHIGDSISVRLRSTKSNRDAIGTAVTLECGS
                     LRQTRFLQAGSGFLAQHAKELFFGLGATEGAARATVRWPDGQEQQFENLPRNHRIEIE
                     EGSPTVSAKPFAPAAKKWDKVRSYALPEQWPLQTQTWLIEPLQAPSFSLPDGEGNLRT
                     LESFRGKPLLIACWSTDSPASAEALRNLQKEGAKLAATGVTLLGLNFDRVASGRQARE
                     ASRALSFPQVFATDEVAGIYNLLYRHLFDRHRDLPLPTSFLLDAGGKIVMVFSGALEA
                     AVIATLAAGIPSTEEERRRRALPFPGELVQDTFHRNAFTLGVAMVQHGYLQQAKASFL
                     QAVENRPADADAWYNLGTLSLRTNDPVQARKYLESTLKLRPDYPEAWNNLGMVAAHEG
                     AASEAIADFERAIQLRPDYAVAMLNLGNMYRHEHAWDQAERYLQKALALQPNDPESNF
                     GLGMLYAQQQSDDRGEQYLRRAIELRPDYAEALNNLGVLLAREQKLTEAENEFAAAIK
                     AAPEFDQSYLNLARLFVVQNQKARAQEILNTLLRLQPDNNAARQALQMLQSAP"
     gene            5137..>5544
                     /locus_tag="VHE33_15205"
     CDS             5137..>5544
                     /locus_tag="VHE33_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HVT98851.1"
                     /translation="MILPRRRFLGSAFLMSGALLEVLTTPVWKWGGALLRAEVKPAAP
                     SSPVQFIDIAEQAGLSTPNVWGGVEHKRIIIETKGSGIAFFDYDNDGWLDIYLTNGSR
                     FDAHWPAGKEPTTHLYRNNRDGTFTDVTAKSGLG"
BASE COUNT         1118 a         1618 c         1700 g         1108 t
ORIGIN      
        1 aagaagaaga atttgtttct gaggatgggg ccgcccgccg ttgccccaaa ctggttaaaa
       61 cgatattcgc ccttcaccgg gtttgccttg ttgttctcaa accagtcggc agcatcgagc
      121 ttgtcgttac ggaagaactc ccagacatca ccatggaagg agttggttcc ggatttaacg
      181 gtggcgttca acacggcacc agcggcacgg ccgagttggg cgctgtaatc ccccgtctgc
      241 accttgaatt cctgaatggc atcaatcgga ggcaggatga cgtagttcgt gccattgagg
      301 aagtcgacgt tatcggagtt gttgtcgatg ccgtcgagca ggtagttatt ctgcgcagga
      361 cgctcgccat tcgcagagaa tgcgccgctt gctgcgttgc cgcgcgtatc cgcctgcggg
      421 ctctgcatgc cggctcccaa ctgcgccagg aaggtgaaat tgcggccatt gagcggcaga
      481 ttgttcaccg actcgcggcc gatcacctgg cccgtggacg cctcctcagt ctgcaacagc
      541 ggaggagctt cggtgacctg caccgtttct gacgcctggc cgaccttgag ctggatgttg
      601 accgtcagcg gcgtgccgac gttcaccgtg aggttcgatt gcgtggtttt cgagaacccc
      661 tgcgcagtta cggtgatctc gtagtgacca gcgcgcaccg gcgcaaacgt gtaggtacct
      721 ccggcggctg tgctcgtttg caccgtgatg ccctgatccg tattcagcag cgtcacctga
      781 gcgttgggaa cgacggctcc ggtcgaatca acgaccgttc cgctgatgga gccttcgtcg
      841 acctgtgcga acgccgcgcg gctggggagc aaaaggcagc agcaggtgat gatgacgcac
      901 gctacgactc caaatgagca cacagccttc ctgtttccgc gcatcctggt acctccgaaa
      961 agagatgaca actggggact accgtgggac ccagaccgct cctctgcaag acgtgtctgc
     1021 agcggggctg aactaccttt gaaatgaaag ccgttacatt ttggcgggtc ttaacccgct
     1081 tggggactat cggtcgatga ctctagagca tccgctttgg ggttgtcaac ctaaagtcgt
     1141 aggaagccac ccgacatcgg gtgtaaagcg cgtcaggggt tcctcgaaag tcacctcatc
     1201 cagccggtgg tatcatcgcc attctcagcc tccatgaccg tctcccaaag aagcaggaac
     1261 ccaacccttt cacgcagaag attgctcaag gggatgggac tggctccact gctcctgcgc
     1321 ccttccccca tcactggatt tggccggttg tggttgccag gaatgccgga ctgggcggcg
     1381 gggctgtcct ctgcagaggt gcaactgcat ccgcattatc cgcaggagtc gcccctggct
     1441 gccgtaatgc ggctggtaga gccagggctg gatggctatc tcaccgaaaa atacgccgcc
     1501 cagattgaag cagtcttaga taactggggc cgcgcacttt gcggctccgg tgattcacgc
     1561 gcagtgctcg ccgcttttgc gagccccgca atcgaagcgt ccgattttgg ccagaagaag
     1621 gagaggccgc tgcgctccgc ctttggtgtc accgtgctga ggcgggactt cgtccaatcc
     1681 gtgacccagg gtcgcgatgc atttctgagc gcgatcgcgg tgtggctcgg agaaattcgg
     1741 cgcgtggaga cggccgaatt cgaaatctac ggcattgaaa cgatcgccgt ctcgcctctt
     1801 gttgtgaaag cggagattcg gtacgacctg gtcgccttgc gcgccgatgg ccggcgggaa
     1861 gagcgcgttg gctcggtgca aaccgaatgg cgttctgaag agcagggctg gatggcgcaa
     1921 cgatggctgc ttcaggaaga aaccgtcagc atcgtggagc agccggcgtt cgtggacgtg
     1981 accgcgcatg cgctgggaga aaccgaatca tggaaagccc agatgctgtt cggcgcggac
     2041 tactggcgga cggtactcga tggcgctgtc ggactggatg tttactccaa taacggcgtg
     2101 gcggcaggcg attttgacgg cgatggtttt gacgatttgt atgtgtgcca gccgtcgggt
     2161 ctgcccaatc gcttgtatcg gaatcgcgga gacggcacgt tcgaagacgt gacggccagg
     2221 gccggcgtgg ccgttctgga taacacggca tgtgctctgt tcgcggactt cgacaatcga
     2281 gggcttcagg atttgctcgt ggtgtgcggc acagggccgc ttcttttcat gaaccagggc
     2341 aacggcacgt tcgcgctcaa gcgagacgct ttccggttcg ccggcgccgc ggagggcacc
     2401 ttcacccatg ccgctgcagc ggactacgac ggcgacggtc gtctggactt gtatctgtgc
     2461 acttatcagt actatctggg tctggatcag tatcactatc cggttcctta ctacgacgcg
     2521 cgcaacgggc cgcccaattg cctctttcac aacgagggcg gacacgtgtt tgtggaagcg
     2581 acggagaagg ccggcctgaa tatccagaac aatcgctaca gcttcgcctc cgcctgggga
     2641 caggccggtg ggggcggcaa gccggatctg tttgttgcaa acgatttcgg tagtagccaa
     2701 ctctaccgaa acaacgggaa cggaaccttt accgacgcat cggaagcagc tcacgtggaa
     2761 ggcgtcggcg ccggcatgag ttgctgctgg cacgatttcg acaacgacgg gcgccaggac
     2821 atctacgtac cgagcatgtg ggaagcggcg ggacagaggg tcaccgaaca aaaacagttt
     2881 catcccgatg cgcccgagag gatccgtgca ctctatcgca ggcacgcgcg cggcaatgct
     2941 ttgtacagga atcgcggaga cggggttttt gaaaatgtgg cccaaaccgc cggcgtcgag
     3001 atggggcgtt ggtcgtggtc ttccgatttc tgggatttcg atcacgatgg gctgcccgat
     3061 ctctatgtga cgaatggata cgtctccggg gcggaccgtg ccgacctcgc cagcttcttc
     3121 tggcggcagg tcgtggctca ttctcccgaa gacgcatccg cgtccacctc ctacgaacag
     3181 gcgtggaatg ccatcaacga gttgattcgc tccgatcgca cgtggcacgg ctacgcgcgc
     3241 aatgtgatgt tcgcgaataa cggagacgga actttcgcgg agatttcagg accagcgggc
     3301 ctggactttg cccaggattg ccgcagcttc gcgcttgccg atctggatca cgatggccgc
     3361 ctggaggtcg tgctcaagag ccgcaatgca ccgcagcttc gtattctgcg aaacgcgatg
     3421 ccgcacatcg gcgactcaat ttcggtccgg ctccgcagca cgaagagcaa tcgcgatgcg
     3481 atcggtacgg ccgtgactct cgaatgcggc tcactgcggc agacgcgttt tttgcaggcc
     3541 ggttcaggat tcctggcgca acacgctaaa gagctgttct tcggcctcgg agcaacggag
     3601 ggcgccgccc gggcgacggt tcgctggcct gacggacagg aacagcaatt cgaaaatctg
     3661 ccgcgcaacc accgcatcga gatcgaggaa gggtccccca ctgtctccgc aaagccattt
     3721 gctcccgcgg cgaaaaaatg ggacaaggtt cgaagctatg ccttacccga acaatggcca
     3781 ttgcaaacac aaacgtggct gatcgagccg ctacaggcac catctttttc tttgcccgat
     3841 ggcgaaggca atctgcgcac gctcgaatcc ttcaggggaa agccgctgct catcgcctgt
     3901 tggtcgacgg attcgcctgc atcggcagag gcgctccgga atctgcagaa agagggggcg
     3961 aagcttgccg ccaccggggt gacgttgctg ggattgaatt tcgatcgcgt cgcgtcggga
     4021 cggcaggcgc gcgaggcctc gagagccctg tcgttccctc aggtatttgc gactgacgag
     4081 gtagcgggga tctacaacct gctctaccgg catctgttcg accgtcatcg cgatctgccg
     4141 ctgcccacat cgtttttgct cgacgccggc ggaaagatcg tgatggtctt cagcggggcc
     4201 cttgaggcgg cagtcatagc aacactcgcg gcggggatcc cctctacgga ggaggagcgg
     4261 aggcggcgcg ccttgccgtt tccaggagaa ctcgttcagg acacatttca tcgcaacgcg
     4321 ttcaccctcg gtgtcgcgat ggtgcagcac ggctatttgc agcaggcgaa ggcgagcttt
     4381 ctgcaggcgg tggagaaccg tcccgctgac gccgacgcgt ggtacaacct gggcacgctg
     4441 agccttcgca ccaacgaccc ggtgcaggcg aggaaatatc tggagtcgac cctgaaactt
     4501 cgacctgatt atcccgaagc gtggaataac ctgggcatgg tggccgccca cgaaggcgca
     4561 gcatccgaag caatcgcaga tttcgagcgg gcgatccagt tgcgtccgga ctacgcggtt
     4621 gcaatgttga acctcggaaa tatgtaccgg catgagcatg cctgggatca ggccgaacgg
     4681 tatctgcaaa aggcgctcgc gcttcagccc aacgatccgg aatcgaattt tggtctcggc
     4741 atgctctatg cgcagcagca gtccgacgat cgcggggagc agtacctgcg cagggcgatc
     4801 gaactgcgcc ccgattacgc cgaggcattg aacaatctcg gcgtgctgtt ggcacgtgag
     4861 cagaagctga ccgaggccga gaacgaattt gccgctgcaa tcaaagccgc cccggaattc
     4921 gaccagtcgt acctgaacct ggcgcggctc tttgtggtgc agaatcaaaa agcccgcgcg
     4981 caggaaattc tcaatacact cctgcgactg caaccagaca acaatgccgc cagacaggcg
     5041 ctgcagatgc ttcagtctgc gccttaagcg cgcagttccc gccacggtct ctgcaggtcg
     5101 ctcctcgaat gcaatccttc tataatcggc catcatatga tcctgccgcg gcggcgcttc
     5161 ctgggctctg cctttctcat gagtggcgcg ctgctcgaag tgctcacgac tccagtgtgg
     5221 aagtggggag gagctcttct cagggcagaa gtgaaacccg ccgccccgtc gtcgcccgtg
     5281 caattcatcg atatcgccga acaggcggga ctctcaacgc caaatgtctg gggcggcgtc
     5341 gagcacaaac gcatcatcat tgaaacgaag ggcagcggaa tcgccttctt cgactacgac
     5401 aacgacggtt ggctggacat ctacctgacc aacggaagtc gcttcgatgc ccattggcct
     5461 gcggggaaag agcccaccac gcacctctac cgaaataacc gcgacggcac atttaccgac
     5521 gtcacagcga aatcgggatt gggc
//
LOCUS       DASZYN010000588         6633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_797875, whole genome shotgun sequence.
ACCESSION   DASZYN010000588 DASZYN010000000
VERSION     DASZYN010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6633
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797875"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VHE33_15210"
     CDS             complement(<1..971)
                     /locus_tag="VHE33_15210"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HVT98852.1"
                     /translation="MPDLLFEAGMEEIPARMIGAAEAELTERVVELLRRERLLDQEHE
                     IHSYSTPRRLAVQVNGVRARQQDATREVTGPSWAVAFKNGQPTPAALAFARKVGLTED
                     QLREATFEEEHLRGTDLNSELLHRAQVKGKLEDQNSGGTRLFKVTSTKGEYLAAMVRD
                     KGRAATEVLPDLLAGELAAVYWPKNMYWRPLKVETFVRPVRWLVALLDHAILPVEFAG
                     VRAANLSWGHRILHGDTPVTIDHPDDYVGVLEAANVTADVKLRQHRIRKAMDLETRAV
                     AGARWREDASLLETVTHLTEWPSAVLGNFDREYLALPEEVLVTVMRD"
     gene            complement(974..2008)
                     /locus_tag="VHE33_15215"
     CDS             complement(974..2008)
                     /locus_tag="VHE33_15215"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HVT98853.1"
                     /translation="MLTPIPTPSVALKALTFQELLLRLQTFWAERGCVIAQPYDVEVG
                     AGTMSPDTFLRVLGPDPVAIAYTQPSRRPADGRYGENPNRLYKHTQLQVILKPPPADI
                     QDIYLQSLEAIGIRLREHDIKFEEDNWEWPVGGAWGVGWQVMLDGLEITQFTYFQQCG
                     GMDLDPISGEITYGLERIAAFLQDVDSLYDVVWAVDPRTGKETTYGDIRLEGELQLSV
                     YNFEAADVSKLWEHLRLYEAECQALLDAFHQQFKVDKAAERKEKIHDRLVHDGASPPD
                     EDLRVALRRFPVLGAYELCLKCSHLFNLLDARGAISVTERVAVMARIRNMVVGVAKAY
                     AMQRQRAGAR"
     gene            2132..3094
                     /locus_tag="VHE33_15220"
     CDS             2132..3094
                     /locus_tag="VHE33_15220"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HVT98854.1"
                     /translation="MSAPRRISGHVRIGISGWRYTPWRGVFYPPKLPQRKELAFAASQ
                     FPTIELNGSFYSLQRPSHYEQWAAETPPEFVFAIKGSRFITHMRKLRQVRTALANFFA
                     SGILKLDSKLGPFLWQFAPNFRFNPDLLDEFFTLLPRDTQAAADLACEHDPWMKGRVC
                     LETPARRPLRHAIEIRHESFVCPEFIRLLRRHKIALVCADTVEWPRLLDVTADFLYLR
                     LHGSEVLYASGYDGIALDVWAERITAWARGHEPRESWKEGHAPDPARTHVPEGVYASF
                     APAPKRTKRDVYVYFDNDAKVRAPFDAQGLIERVNRLLLRKPSI"
     gene            3349..3741
                     /locus_tag="VHE33_15225"
     CDS             3349..3741
                     /locus_tag="VHE33_15225"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HVT98855.1"
                     /translation="MPRKTSQTLTEAELRIMQVLWLKGPGTVQHILDALPTNPALAYN
                     TVLTTIRILERKGYIGHDKDGRAHIFRALVAEEEASRSEIRHLVSRFFRDSHEDLVLN
                     ILEDRGIDATELERLRKMLERPNGDTAQ"
     gene            3738..5195
                     /locus_tag="VHE33_15230"
     CDS             3738..5195
                     /locus_tag="VHE33_15230"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HVT98856.1"
                     /translation="MMTFAQLAVERVLNSLPEGLLIAVCAWLLLRVMGRQNSGTRFAV
                     WLVALASVVTLPFVRSLGSTHDNLSALLPQAHPEISISVFWASAFFVLWSLIATVALA
                     RVAAGLWQVRQIRRSCIEIPAADLDPEFAALLSDSKRPVRLLVSEKARVPAALGFRKP
                     AIVLPAWALSELSTDDLRPILIHELAHLRRRDDWTNLLQKTIRALLFFHPAVWWIDAR
                     LSLEREMACDDAVLAATGNPRAYAGCLIDLLEKGCARRGWSMAQAAVAHARDASIRIA
                     RILRVGPAVNTRVGRVALSAAASLSLVCTAIALCAPQFVEFVPLQSASTAQLNEPLVD
                     NVSFAPSHSAVVPATFHPNQKPASAHRPVAKRTRSAHAAPLRQASLTSKHPVQNPVVL
                     AKVTEFTTTTEPAPVPVMIVLRSTESATAGPAQLRPTGASPSDSNVRILQSTENPALQ
                     IQIVQIVDPATGMPTQILRIILVVPQQPGSPSQSI"
     gene            5252..>6633
                     /locus_tag="VHE33_15235"
     CDS             5252..>6633
                     /locus_tag="VHE33_15235"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HVT98857.1"
                     /translation="MNFEKISSLLKRRVAVPVVALGLAAGVGAWTFARPASAAAPAAA
                     PMDESSVGPLLSFDQAMEALTARVRPAVVNVSVTAKVKHPRVMMFNGEDQNDDQGQDQ
                     QQQQFQQFFGPFGQFFQGPMQRQQQPNIEHGLGSGVLISPDGYIVTNNHVVDGATDIS
                     VTLSNRDMYPAKLIGTDPLTDLAVIKIEGHDFPSIPWGDSAKLEPGQLVLAFGNPYGF
                     RFSVTRGIISALDRGNPDSDRRKPGEFIQTDAAINPGNSGGALVDARGQLIGINTFLY
                     SPTGAFSGMGFAIPTKIAQPVVNTLMKDGKVSHGYIGVSIEDVTPQNAKFFEIKKAVG
                     AVVSEVTPDSPGGKAGLKTGDVITEFNGKPVSDAGELQMETSLTPPGGAIHLQVMRDG
                     KSIDMPVTLGSMPGAHEPVVAQNGTGKGRWGVGLGDLTPDVRQQINAPASVHGAVIQN
                     VRPGSPADNAG"
BASE COUNT         1236 a         2461 c         1676 g         1260 t
ORIGIN      
        1 tgatcgcgca tcaccgtcac cagcacctct tccggcagcg ccagatattc gcgatcgaaa
       61 ttccccagga ccgcggacgg ccactcggtc aggtgcgtga ccgtttcgag cagcgatgca
      121 tcttcgcgcc agcgcgcacc cgccacagcc ctcgtctcca ggtccatcgc cttgcgaatc
      181 cggtgctggc gcaacttcac atccgctgtg acgttggctg cctccagcac gcccacatag
      241 tcgtccggat gatcgatggt caccggcgtg tcgccatgca ggatgcgatg gccccagctc
      301 agattcgcag cgcgcacacc cgcgaactcc accggcagga tcgcgtgatc cagcagcgcc
      361 accagccagc gtaccgggcg cacaaacgtc tccaccttca gcggccgcca gtacatattc
      421 ttcggccagt acactgccgc cagttcgccg gccagcagat ccggaagcac ctccgttgcc
      481 gcccggccct tatccctcac catggccgcc agatactcgc ctttcgtact cgtcactttg
      541 aagagccgcg ttcccccgct gttctgatct tccagcttgc ccttcacctg ggcgcgatgc
      601 agcagttcgg agttcagatc ggttccccgc aggtgttcct cttcaaacgt ggcttcccgc
      661 aactggtcct cagtcagccc cactttacgt gcaaacgcca gcgccgcagg cgtcggttga
      721 ccattcttaa atgccaccgc ccacgaaggt ccggtcacct cgcgcgtcgc atcctgctgc
      781 cgtgcccgca caccgttcac ctgtaccgcc agccgccgcg gggtcgaata gctgtgaatc
      841 tcgtgctcct ggtcgagcag ccgctcgcgc cgcaacaact ccaccacgcg ctcggtcagc
      901 tccgcttccg cggccccgat catgcgcgcc ggaatctcct ccatccccgc ctcgaacaaa
      961 agatccggca tctctatcgc gcccccgctc tctgtcgctg catcgcgtac gctttcgcca
     1021 cacccaccac catattgcgt atccgcgcca tcaccgccac ccgttcggtc actgaaatcg
     1081 ctccccgcgc atccagcaga ttgaacaggt gcgagcactt cagacacagc tcataagcac
     1141 ccagcaccgg gaaacgccgc agtgccaccc gcaaatcctc atccggcgga gacgcgccat
     1201 cgtgcaccag ccgatcgtga atcttttcct tccgctcggc cgccttgtcc accttgaatt
     1261 gctgatgaaa ggcgtccagc aacgcctggc actccgcttc atatagccgc agatgctccc
     1321 aaagcttgct cacatccgct gcctcgaagt tgtacaccga aagctgcagt tcgccttcga
     1381 gacgaatatc gccatacgtc gtctctttgc ccgtccgcgg atcaaccgcc cacaccacgt
     1441 cgtacagcga gtccacatcc tgcagaaacg ccgcgatgcg ctccaggccg taagtgatct
     1501 ccccggagat cgggtccagg tccatccccc cgcactgctg gaagtacgta aactgcgtga
     1561 tctcaaggcc atccagcatc acctgccagc ccacgcccca cgcgccgccc accggccact
     1621 cccagttgtc ttcttcgaac ttgatgtcat gctcgcgcag gcgaatgccg atcgcctcca
     1681 gagactgcag ataaatgtcc tgaatatcgg ccggcggcgg tttcaggatc acctgcagct
     1741 gtgtgtgctt gtacagccgg ttcggattct ccccgtaccg cccgtcggct ggacgccgcg
     1801 acggctgcgt ataggcgatg gccaccgggt ccggtcccag aacccgcaaa aaggtgtccg
     1861 gcgacatggt ccccgcgccc acctccacgt cataaggctg cgcaatcacg cagccacgct
     1921 ccgcccagaa ggtctgaagt cgaagcaaaa gttcctgaaa cgtcagcgcc ttcagcgcaa
     1981 cagacggtgt aggtatgggc gtcaacatgg gcacctggaa agttcgaggg tttgctctaa
     2041 cgattctatc ggcacgaagc tttcagcgca ttatgggacc gcccgggcct ccccatgcaa
     2101 cggtccgcgc cctttgtgat tccatctcac cgtgagcgct ccccgccgca tttccggtca
     2161 cgtccgcatc ggcatctccg gctggcgcta cacgccgtgg cgcggcgtct tctacccacc
     2221 caaactgccg cagcgcaaag aactcgcctt tgccgcctcc cagttcccga ccatcgagct
     2281 caacggcagc ttctactccc tccagcgacc ctcccactac gaacagtggg ccgccgaaac
     2341 cccgcccgag ttcgtttttg ccatcaaagg ttcccgcttc atcacgcaca tgcgcaagct
     2401 ccgccaggtt cgtaccgccc tggccaactt cttcgcctcc ggcatcctga aactcgactc
     2461 caaactcggc cccttcctct ggcagttcgc cccgaacttt cgcttcaacc ccgatctcct
     2521 cgacgaattc tttaccctcc tgccccgcga tacccaggcc gcggccgacc ttgcctgcga
     2581 acacgatccc tggatgaagg gccgcgtctg cctcgagacc cctgcgcgcc gacccctccg
     2641 ccatgccatc gaaatccgcc acgaaagctt cgtatgcccc gagttcatcc gcctgctgcg
     2701 ccgccataag atcgccctcg tctgcgccga cactgtcgaa tggccccgcc tcctcgacgt
     2761 caccgccgat ttcctctacc tgcgcctcca cggctctgaa gtcctctacg ccagcggcta
     2821 cgacggtatt gctctcgacg tctgggccga acgcattacc gcatgggccc gaggacacga
     2881 acctcgggaa tcctggaaag agggccacgc gccagaccca gcccgcaccc atgtgccgga
     2941 aggcgtctac gcctcattcg cacccgcccc caaacgcacc aaacgcgacg tctacgtcta
     3001 cttcgataac gacgctaagg ttcgcgcccc cttcgacgcg caagggctca tcgaacgtgt
     3061 caatcgcctg ctcctgcgca aaccgtcgat ttgaggttct ggatttcacg aagggcctcg
     3121 attatggccg tgaatcagtg cggcaaccca tgccatgaac gaaggattcg aactcgctcc
     3181 gaagtcttgc ctgggatagt gaagcgcgcc gggactttga tgcagtcctt tggaatcagc
     3241 cgcttaccgt tttatgattt gcaaaaatcc ccgctccgca tgataaaact aaattcttag
     3301 ttacttctgc cgtcttcttg cgtccaacaa tctgaaggga ctttaaccgt gccgcgcaaa
     3361 acctcccaga ccctcaccga agccgagctc cgcattatgc aggtgctctg gcttaagggt
     3421 cccggaaccg tccagcacat ccttgatgcg ctgccgacca accccgcgct cgcctataac
     3481 actgtcctca ccaccatccg cattctggag cgcaagggct acatcggcca cgacaaagac
     3541 ggccgcgccc acatcttccg tgccctcgtc gcagaagaag aggcaagccg ctcggagatt
     3601 cgtcacctcg tcagccgctt tttccgtgac tctcacgaag acctggtcct caacattctc
     3661 gaagaccgcg gcatcgacgc caccgaactc gagcgtctcc gcaaaatgct ggagcgcccc
     3721 aacggagaca ccgcgcaatg atgaccttcg cgcagttggc cgtcgagcgg gtgctcaatt
     3781 ccctccccga gggtttgctc attgctgtgt gcgcctggct gctgcttcgc gtcatgggcc
     3841 gccagaactc cggtacgcgc tttgcggtct ggctcgtcgc gctggccagc gtcgtcacct
     3901 tgccctttgt ccgcagcctc ggctcgaccc acgacaatct gtcagcattg ctccctcagg
     3961 cgcacccgga aatatcgatc tccgtattct gggccagcgc cttctttgtt ctttggagcc
     4021 tcattgccac ggtcgccctg gcgcgtgtag ccgccggcct gtggcaggtt cggcaaattc
     4081 ggcgcagctg catcgaaatc cccgcagcgg acctcgatcc tgaattcgct gctctcctct
     4141 cggactcgaa gcgacccgtt cggctcctcg tatcggaaaa agcacgtgtt cccgcggccc
     4201 tcggcttccg caagccggcc atcgtgcttc ccgcatgggc cctcagcgaa ttgagcaccg
     4261 acgatctccg acccatcctc attcacgagc tcgctcacct ccgccgccgt gacgactgga
     4321 ccaaccttct tcagaaaacc attcgcgcat tgctgttctt ccatccagct gtctggtgga
     4381 tcgacgcgcg gctctcgctt gagcgcgaga tggcatgcga cgacgccgtt ctcgctgcta
     4441 ccggcaatcc ccgcgcgtac gcaggatgcc tcatcgacct gctcgaaaaa ggctgtgccc
     4501 gccgcggatg gtcgatggcc caggccgccg ttgcccacgc ccgcgacgcc tccatccgca
     4561 tcgcgcgcat ccttcgcgtc ggccctgcag tcaacacccg cgtcggacgc gtcgctctca
     4621 gtgctgcggc gtccctctcc ctggtctgca cagcaatcgc gctctgtgcg ccccagttcg
     4681 tcgagtttgt ccccctgcaa tccgcgtcca ccgcgcagct gaacgagccg ctcgtcgaca
     4741 acgtcagctt tgcaccatcg cattccgctg tcgtcccggc caccttccat cccaatcaga
     4801 aaccagcgtc ggctcaccgc ccagtcgcga agcgcacgcg cagcgcgcac gcggctcctc
     4861 tccgtcaggc ctctctgact tcaaagcatc cggttcagaa cccggtcgtc ctggcgaaag
     4921 tcacagagtt caccaccacc actgagcccg caccggttcc ggtgatgatc gttcttcgct
     4981 cgacggaatc tgcaaccgcc ggtcccgcac agcttcgccc caccggtgcc agtccatcgg
     5041 acagcaacgt tcgcatcctg cagagcaccg aaaaccctgc tctgcaaatc cagatcgttc
     5101 agatcgtcga ccccgccacc gggatgccga cccagatcct caggatcatc ctcgtcgtcc
     5161 ctcagcagcc tggatcgccc tcgcagtcga tctaaaaaaa tttggactca acaactaatt
     5221 cattagttta caaacaggag aaatacaccc catgaacttc gagaaaatca gctccttgct
     5281 taaacgccgc gtcgccgttc cggtcgtcgc gctgggcctt gctgccggtg tcggcgcctg
     5341 gacgttcgca cgtcccgcca gtgctgccgc tcccgccgcc gcgcccatgg atgaaagcag
     5401 cgtcggtcct ctgctttcgt tcgatcaggc gatggaagcc ctcaccgcgc gcgttcggcc
     5461 cgccgtcgtc aacgtcagtg tcaccgcaaa agtcaaacat ccgcgcgtca tgatgttcaa
     5521 cggagaggac cagaacgacg accagggcca ggatcagcag cagcagcagt ttcagcaatt
     5581 cttcggcccc ttcggccaat tctttcaggg gccgatgcag cgccagcagc agcccaatat
     5641 tgaacatggt ctgggcagcg gcgtcctcat ctcacccgac ggctacatcg tcaccaacaa
     5701 ccacgttgtc gatggcgcca ctgacatcag cgtcaccctc agcaatcgcg acatgtatcc
     5761 ggccaagctc attgggaccg atccgctcac cgatctcgcc gtcatcaaga tcgagggcca
     5821 cgacttcccc agcattccct ggggtgactc tgcgaagctc gagcccgggc agctggtcct
     5881 tgccttcggc aatccctatg gcttccgctt cagcgtcacg cgcggcatca tcagcgcgct
     5941 cgaccgcggc aatcccgact ccgatcgtcg caagcccggt gagttcattc agaccgacgc
     6001 cgccatcaac cccggcaatt ccggcggagc gctcgtcgac gcgcgcggcc agctcatcgg
     6061 catcaacacc ttcctctact cgcctaccgg cgctttctcc ggcatgggct tcgccatccc
     6121 cacgaagatc gcgcagcccg tcgtcaacac cctcatgaag gacggcaagg tcagccacgg
     6181 ctacattggc gtctccatcg aagatgtcac cccgcaaaac gcgaagttct tcgaaatcaa
     6241 gaaggccgtc ggcgccgtcg tttccgaggt cactcccgac tcacccggcg ggaaagcagg
     6301 cctgaagacc ggcgatgtca tcaccgagtt caacggcaag cccgtctccg acgcgggcga
     6361 gttgcagatg gaaaccagcc tcacgcctcc cggcggtgcc attcaccttc aggtcatgcg
     6421 tgacggcaag agcatcgaca tgcccgtcac ccttggctcc atgcccggcg ctcacgagcc
     6481 ggtcgtcgcg cagaatggca ccggcaaagg ccgctggggc gtcggtctcg gcgacctcac
     6541 tcctgacgtg cgccagcaga tcaacgcacc ggccagcgtc cacggtgcgg tcatccagaa
     6601 tgtccgcccc ggcagccctg ccgataacgc cgg
//
LOCUS       DASZYN010000589        24380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1054774, whole genome shotgun sequence.
ACCESSION   DASZYN010000589 DASZYN010000000
VERSION     DASZYN010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 24380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24380
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054774"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VHE33_15240"
     CDS             complement(<1..638)
                     /locus_tag="VHE33_15240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT98858.1"
                     /translation="MAAETTTQDHARPQPTGCATDMIVVEDLWKTYDMGSEQVNALQG
                     INLHICRNEYVAIMGPSGSGKSTLMNLIGCLDTPSSGKYWLNDHLVSELNDDELARIR
                     NKEIGFVFQTFNLLARATALHNVELPLIYNGTPAEERIQRAKAALTAVSLDTRMNHKP
                     NELSGGQRQRVAIARALVNNPSIILADEPTGNLDSKTGVEIMALFDDLQRKG"
     gene            complement(697..2091)
                     /locus_tag="VHE33_15245"
     CDS             complement(697..2091)
                     /locus_tag="VHE33_15245"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HVT98859.1"
                     /translation="MKKILLIIGAVVVFGAIVGFTVIRAQSGYTKVLIGTVSKENLVS
                     IVSATGQVRPKVYVNVGANAMGRVTHFYVNEGDHVKKGQILATIENVQQHANVAAQQA
                     NIDAARTDINSYIAAEKTAEANVAHAKADLDQKKLDYQRAQALFNAQVMSKQDFDAKK
                     AAYDLDVASLAQAEAGLAQARAQTASARGHLQTQQATLQVNQDLLDRTIAVAPFDGIV
                     TNKPIREGEMVVMGIQNAEGSTLMTLADMSVITAEVKVDETDIVNVRNGQTADVTIDA
                     LPGKIFKGHVTEVGDQALLRSSGQSTSQSTTGVEEAKDFKVVVTLDEPSNDLRPGLSC
                     TAKITTAKKNDIIAVPIQALTMRDPKHLNDKTPTSSGSTVSAATNNSQPTQEVQGVFV
                     VSKVNGKDRVHFVPVTTGITGATDIEVTSGLKTGDRIVTGTYRVLRDLKENSLVKQDS
                     SLAAPQKDSSSSSS"
     gene            2423..4147
                     /locus_tag="VHE33_15250"
     CDS             2423..4147
                     /locus_tag="VHE33_15250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HVT98860.1"
                     /translation="MSWSRFSLPSAILITVLAGAGPAVTRAWSQDQDSAQSQQEQQDP
                     LKRPLSDKQRRQQQKELHSELETAYKKWLDQDVRWIITDQERKAFMSLSNDEERDAFI
                     EQFWRRRNPNPDSPENEYREEHYRRIAYANEHFAAGKPGWMTDRGMIYIKFGKADSVD
                     SHPSGGQYERPIDEGGGETSTFPFETWHYRYLAGIGDNIDIEFVDSCMCGDYHMTIDR
                     SEKDALLHVPNGGQTLYEQMGMAKKADRFKGGLESLGEGPGTSGNQSKEFDRIELYAK
                     LQAPPEIKFKDLSLAEFLTSKKMLTGPFFPFEVRTDFVKVTDDTVLVPITLQIKNKDI
                     TFSTKDGVSKGNVSIVGRISTITDRVVQSFEDPVEAGPYPAELLPREMAGASLYWKAF
                     PLRPGRYRIDIAIKDVNNPDHVGVWAQAITVPRYDDDHLSTSSLILADKMQRVPSKDI
                     GAGNFIIGNTFVRPRVTTNPASPATFHRNQNLNFWMQVYNLGLDEKSKQNSARIVYQI
                     LNATTNKSILDTTEDSKKLSPNSDQLTLEKSLPLASLEPGKYIVKISVDDDVTRQQIA
                     QSAPFTVE"
     gene            4267..5976
                     /locus_tag="VHE33_15255"
     CDS             4267..5976
                     /locus_tag="VHE33_15255"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVT98861.1"
                     /translation="MSRRPTICVPLLIALAVLCADAPRASAQGNSLSGVIRDSRGAPQ
                     PGVAVELLRPDLSVVSEVFADAHGRYRIEGILPGIYEIKASGAMFLPTLRENLQVLTG
                     TKLVVNLTLNTLYEAFRWLPAKPRQADEPKDDWTWTLRLSANRPLLRLLEDGPLVVVS
                     NGDGSAPELKARVTVRGGESGFGDGGLHNDFELERTPEDGRAMIFRADLSHGSPGQLI
                     DPALNAMVGYEQQLALGRTFRAVGAVETRPDIAGGPLGQGLQAIELRSGQTMNFGPGV
                     QAEFGNEMQGIRLGDTILANHPFAAVTVHRGAAYVGYRMSTAPGIQTVEDLDREATVE
                     PRLGERNGRLTLEQGLHQELVAGKGEGDTRVKMAVWHEQVKNPVVTGGGNISAADWAG
                     GNLLYDETSDLLRATGQSYSGEGMLTEVEQRVNGSTWISFEVALGDALAMADAASGTD
                     SLDEALNGMKARETPMVGAGIRGKVAATGTQWRASYRWQNPRTLTAVDAFSSGASSPY
                     LSFLVRQPIRYRRMIPNGVEALVDVRNLLAEGYRPFVSSDGSVLYFAEAARCVQGGLS
                     FTF"
     gene            complement(6079..7089)
                     /locus_tag="VHE33_15260"
     CDS             complement(6079..7089)
                     /locus_tag="VHE33_15260"
                     /inference="COORDINATES: protein motif:HMM:NF018828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98862.1"
                     /translation="MIIVHSYPVAVALCILTMFCWGSWANTLKMEPRSYGFGLFYWDQ
                     AIGYFLLTLILALTFGSLGSEGRTFFADLAQGSGASYAWALLGGVVFNLANILFMAAL
                     DLAGMAVAFPIAIGIALVEGVLVNYVAQPKGNPLLIFGGVTLIVIAIILDGLAYRACQ
                     GPTSGPGQTRKGILLAVSGGVLMGLFYLLLQRSVSLDFIHLNPGKFGPYAAVVVFAFG
                     SGVSNLLFNTYFMAHPVKGEKVTYRDYFKSGTMPYHLIGVLGGVIAGIGVTSNIVASK
                     AASPAIAYGLGQGATLIAAIWGVCVWKETKGASKGTQVMIGAMFLFFITGLTLLVESM
                     LQ"
     gene            7411..8550
                     /locus_tag="VHE33_15265"
     CDS             7411..8550
                     /locus_tag="VHE33_15265"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HVT98863.1"
                     /translation="MAAITQTAPAATATARVLSLDIARGITIAFMIMVNNNGAAAYHP
                     FTHSAWNGWTPTDLVFPTFVFIVGASIVLAFGKRFESGASRARIAVTIVRRAVILFAL
                     GLVVNGFPHFPLHTLRIYGVLQRIALCYLIAGLFYLWRRDAASKIVAIVVLLVGYWIL
                     MRYVPVPGFGVPTHQIPLLDPDRNWVAWLDRKLLPGRLYEGVRDPEGLLSTFPAIATA
                     LLGVLAGLWMRTKRSREQIATGFLAAAVVLIALGELWSLTFPINKKLWTSSFVLFCAG
                     CSLLLLGILYWCIDVKQWRRPFTQPWIVFGTNAIFAYMLSELLASTLWSVHIRGPEML
                     WTWVYTPFHHIHPPGIGSLLYSIAFTAFCWLATWPLWRRRIFLKI"
     gene            8679..9632
                     /gene="rbsK"
                     /locus_tag="VHE33_15270"
     CDS             8679..9632
                     /gene="rbsK"
                     /locus_tag="VHE33_15270"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HVT98864.1"
                     /translation="MPSSIVVVGSINLDLVCTGERIPAPGETLKGNRFQTFHGGKGAN
                     QAVAVARLGHPVNIIGKVGDDDFGDRLRRGLRDAHVGVRNVFTVSGVSSGVALITVDA
                     AGQNSITVIPGANGQLLPRDLEKSLPQLRSAAIILTQLEIPMETVEYLCATARRFNIP
                     VVLDPAPAGPIPRRILRNVAWLTPNETETCALCGIAPHELTSANVDSVAELLLGRGPL
                     NVIVKMGRRGAYVAGADGLRRMIPAFKVNAIDSTAAGDAFNAGLAVGLVRGMPLVDAA
                     RYGAAVGALSTTRAGAQASMPSVREVSRLLEPARERHRRTR"
     gene            complement(9695..11122)
                     /locus_tag="VHE33_15275"
     CDS             complement(9695..11122)
                     /locus_tag="VHE33_15275"
                     /inference="COORDINATES: protein motif:HMM:NF014649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98865.1"
                     /translation="MATRILRAGLCGIGLDAYWPQFAGLREQLEGYLDQVKARLQGFG
                     AQVESLGLVDTPQRSREAGHACRRADIDVLFLYVTTYALSSTVLPLVQRAQVPVIVLN
                     LQPGAALDYEAFNRLPDRTAMTGAWLAWCSACPVPEIANVLGRAGIPFHQVTGALTDE
                     TSWKEIEEWIRAAQVKAALAETRLGLLGHYYGGMLDVATDTTQVAITFGSHIEMLEVD
                     ELSALRDDCTEDQIAHRVRHFEKHFDLQPGCARSELERAARTSLALDRLVEQHDLQSL
                     ACYYKGTGVSANEDTMSSTILGTSLLTARHIPVAGEYEVKNAIAMKIMDLFDAGGSFT
                     EYYALDLADDIVLMGHDGPGHIAIAEGKTRVRPLEVYHGKVGRGLSVEMSVRYGPVTL
                     LSVVDDREHGFRLLAAHGSSEPGPTLAIGNTNSRYRFPIGARRFVERWNQQGPAHHCA
                     VGIGHLGGLIAKLARLLEIPFVQVC"
     gene            complement(11132..12649)
                     /locus_tag="VHE33_15280"
     CDS             complement(11132..12649)
                     /locus_tag="VHE33_15280"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98866.1"
                     /translation="MQFRMSIVAAAVCFGSCAIMAQETAAKSGTIKIDWDKTVIVSKS
                     TPTLQVVTNPMLNPGSPIHDGAFAALKMLGADYVRFVPWLPYPRIAVAELQPPTTDHT
                     SWDFQYIDPVTKDFLAATEGHSTIVNFSTIPAWMFKTEKPVTYPDDPNQVYWNYTQGT
                     ELRDPSGKELGDYYARLVSWFTKGGFSDENGKRHDSGYHYKFPYWEVLNEVDFEHQMT
                     PEQYTERYDAIVSAIHTVSPETKFVGLALAAPSANPKYFEYFLNAKNHRPGIPLDYVS
                     FHFYATPALDEGPDGWQHTLFNQADGFLAVTRYILSIRDRLSPETKIDTDELGAILPT
                     DGVEIRAGKPLPDHIPRRYWNAAAALYAYLFAELSKMDVDVIGESQLVGYPSQYPSVS
                     MVDYNNGKPNPRCWVLKMIIENFHPGDRLVNTEPLSGDQPQPYVQGFATAQGRKILLV
                     NRSNAEETATVPAGFEHAQFATVDEASGDDPPRTGTLTGPQLTLAPFAVTVLKAE"
     gene            complement(13048..13494)
                     /locus_tag="VHE33_15285"
     CDS             complement(13048..13494)
                     /locus_tag="VHE33_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98867.1"
                     /translation="MKYRGIRSDREFNPYERFDSLIAGCLIVLIQLAAYFPSLDTHYR
                     IITGGVVRGTVKELYSDRPFFQLVWIPVQLALNHKAQKLTAAVRFVEERTLQYFLQFI
                     EDDGLRLSALSHRSAGLGLRVFQTWSHFHRPPEGRLRAAICKRVSP"
     gene            13690..14442
                     /locus_tag="VHE33_15290"
     CDS             13690..14442
                     /locus_tag="VHE33_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98868.1"
                     /translation="MTRRFWEPVFGTPQPVLICLAKGVTYRPDEKLYDLYAQNHPGTF
                     RTEVERSNDPLPLDPHQSILWGQMNLYDDYGIAGGDVSSALRISEYLGSIGKPLQVRI
                     GSNYSFEDLRHSPAVVIGAFNNKWTMDLTAGLHFEFVEQPGRTLAIRESVPGGREWHV
                     PADPATDYGIISRLLDSKTGQFIVTAAGLTGSGTDAAGELATRQEYLEQVLRSAPANW
                     ERGNVQIVFQTSVLDQVPGPPRVIATYYWESP"
     gene            complement(14524..16317)
                     /locus_tag="VHE33_15295"
     CDS             complement(14524..16317)
                     /locus_tag="VHE33_15295"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HVT98869.1"
                     /translation="MANAGPQEAQFDTQHRPITAGGFVKTGPIVFQDVAKAAGLTTWR
                     HSAGTPEKRLILEAKGPGVCLLDFDNDGWLDIYLVNGSNYDALAGKAAPPHAALFRNN
                     HDGTFSDVTEKAGATNDRWGYGCAVGDYDNDGWPDLYVTNYGKNRLYHNNHDGTFSDY
                     ADKAGVTIGAWSAGATFGDYDGDGRLDLFVDGYAKIDLANPPVSGSKGIAYAICQYRG
                     VEVMCGPRGLQGEQDHLFHNNGDGTFTDVSAKLGVDDSRGYYGLGVLFADVNNDGKPD
                     LMVANDSTPNYLYINKGNGKFEDESYISGYGLNKDGREIANMGIAAGDYENNGHLSIV
                     NTDFSDDYNVLFRNDGTGFFTDVSHEVGIAMSSIPFVGFADGFLDYDNDGWKDLLIVN
                     GHVYPQVDEHPEWGQSYAQRTLLYHNLKDGKFELVPAVEGTGLAVVTVGRGAAFGDIF
                     NDGRIDVVINSMDGVPVLLRNVNADHHHWVEFRLIGGPGSPRDAVGATVYLNAAGMRQ
                     RGDVLSGGSYLSSNDLRAHFGLGGSTDAGTAEIHWPSGKKESVRLPAVDRIYTITEGE
                     GITGELCSGKPCAQIKPTVPAHLKTLTAAPK"
     gene            complement(16547..18037)
                     /locus_tag="VHE33_15300"
     CDS             complement(16547..18037)
                     /locus_tag="VHE33_15300"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT98870.1"
                     /translation="MLRFRLIAGLALALTTSSMAQTATTSISSVESLIRSQHYDQALA
                     ALKIAIRKNPENYRLWTLQGICFALQGNDRDALAAFDHAIHIQPTYTAALRGETQILY
                     KSGDKRAIPLLERMLKSDPGDTTAQEMLATLQQHNGDCRDAVSHFEMSRAAIATHPAS
                     LEAYGYCLFQLDRTPEAIAVFRQLVPLLPGSAYPSYNLAVLLVSAHQNDDAVKVLEPF
                     LTPGQTDPDILSLASQAYEAIGNTPRAVALQRQAIVLNPTEASNYVLFAVLCLTHDSF
                     QVGIDMLNAGLKRISDNSALYLSRGVMFEQLGKFDEAEADFRMAEQIDTTQHIAAYAG
                     DLTMIERNDPQKAFERVREQLRAHPNNPLFHLMVAQLILTRAPAPGSADFNEAMQNAL
                     AAAKTKPDLVEAHNQLAGMYMSLNDYQKAIEECRTALQYDPSNEAAMYHLVMSMRHAG
                     HTPEELQPLVQRLAEIHKESLQRETDRKRYRLVEEGSPAADSDGQH"
     gene            complement(18156..20027)
                     /locus_tag="VHE33_15305"
     CDS             complement(18156..20027)
                     /locus_tag="VHE33_15305"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HVT98871.1"
                     /translation="MFIGTAVEEVLPHVRVLVRGALLLSLAAPACCQTQQPVQGGMGG
                     GIASAGIFAPVYDEHRRPITAGGFVDTGTVVFEDATKPAGLSAWRHEMGTPQKKYILE
                     TDGSGLALIDYDNDGWLDIYLVNGSTYDAMAGKREPPHAALFHNNHDGTFTNVAAGAG
                     VTNDRWGFGVAVGDFDNDGWPDIFVANFGKNRLYHNNHDGTFTDVAEKAGVTLGNWSK
                     GAAWGDYDGDGRLDLFVSGYLHYDLDHQASSEDGRASTANCQMRGAPVMCGPRGLPGE
                     PDHLFHNNGDGTFTDVSEFAGVSDPDRFYGFTAVFVDVNDDGKVDLLVANDSQRNYLY
                     INKGNGKFEDQSYVSGFAFNFDGREIASMGLAAGDYLNNGRVDILDTDFSDDYKALFR
                     NDGNASFTDVSRESGIAQIAVPFVGWGDGMIDFDNDGWKDLMMINGHVYPQVDEHDWG
                     TTYAERPLLFHNNGHGRFDYVPPVRGSGLAVVTTGRGAVFGDLFNNGKIDVVINSVDR
                     PPVLLKNVNPDHHHWVELRLMGGARSPRDAIGTTVYLTANGMRLREDVMSGGSYISSN
                     DQRPHFGLANATDAGTAEIHWPSGSIEKVRLPAVDRIFTVTEGKGITGVWCAKQPCS"
     gene            complement(20072..21364)
                     /locus_tag="VHE33_15310"
     CDS             complement(20072..21364)
                     /locus_tag="VHE33_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98872.1"
                     /translation="MEVEAAGRFMNSVLEVPGNRDADAADKLKCDRSAVLGELDNVLS
                     SQHFRSARRSREFLRYVVLNKLDGQSERLKERTIGTDVFKRPPGYPTGEDPVVRVQAG
                     EVRRRLDQYYQETPESPRVRIELPVGSYSPVFHSCTVARDHKSEVQSATRRDRPYLKK
                     PVVRVALFLLFVMLAALLGRAVLNSLRRVDSPRTAFDQFWGPATASHQPVLICVANVS
                     KNGSEAAAPSNGRTDASQREVEAAVTPTSAGQDDPEIVPAGDASVAADLSGLFGKLDR
                     PSQLRIGARITYEDLRSFPAVIIGGFNNRWTMQLLSNLRFTFVRDRGQYMIREQVPGG
                     RVWTTRVGAHGEVLEDFAIVSRLIDSKTGQFTVTVAGIGPRGTQAAGEFVGSAHDLDE
                     GLAKAPTEWPDRNLEIVLHTTVTESVAGPPQVIATYAW"
     gene            21753..>24380
                     /locus_tag="VHE33_15315"
     CDS             21753..>24380
                     /locus_tag="VHE33_15315"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT98873.1"
                     /translation="MKRWTSISIFFVLLSFIFGGSTALAQFSSGVQGVVHDTSGAVIA
                     NAKVIVTDTRLGVSRTAQSGEDGYFRIDGIAASTYSVEIQMSGFQTWKQDGLALQIAE
                     VRTIAPELKVGAVSTSVEVSASAASVDLETPTTGAIISNETLQQTPLPGQNIYGLASL
                     SPGMTGGGIVTGGNDNYTNEYGINLNAAGLRQEENGFQIDDGYTNTPSRGGQTSISPN
                     PEIVDSVNVRTNDFDAQKGRNAGATVDVYTKSGSNDLHGTVDYYFLNDTLSAKTHFQS
                     KVPTFTRNEVGGTLGGPAIKNKLFWFGAIDVLRSSNPNSYTATVETQDFYNWAKANLP
                     NTFGTQILGMAPPQTYPTTGLETVAAYEATSGTPGYYAPPAGIPATMNIIGTIPVSYS
                     STKNGYQWSVRGDYYITNSDRLYVDVIRTKYDSPGGGTRPASVQGNSNSSDFFNANWT
                     HTFSSRLINQTGLNLIRPYGSNITQVATEGIPYVNVNNLAGFGSWAPGNFTQSTYSWR
                     DVMTATIRKHTLKVGVDLFNTREVDHQQGAFTRPTFNFRNLLDLVQDEPVYQSGPNVN
                     LQNHQQAPYDRDYRDLIQGYYAQDSWKMTPRFTLTAGLRYDGMVNFFSINRPILSKFN
                     LGSGSTWNAQVASGIAALAPNNHVLDHNIGGFTPRLGFSWDPFGTGKTALRGGFGMFE
                     DQPPYLHITDSTAANLPNYFNPIADVTAGSPTPKPFLCTPAGAWNMNCPVLDTSNATV
                     DPTTGALRINGVVQRAGMGGYDPNFKMQQVIAWSLSVQQQLQSNLIAELNYSGTEGHH
                     LLVFNNDINRFAGDLIQNNNTLTRLNPYFAGTAYATSNGNSSGQYGSLALTRRYTRGF
                     ALRGIYTYGKVLDEMSFAQS"
BASE COUNT         4906 a         7070 c         7314 g         5090 t
ORIGIN      
        1 ttgccctttc gctgcaggtc atcgaacagg gccatgatct ctacgccggt tttggagtcg
       61 aggttgccgg tgggctcgtc ggcgaggatg atcgatggat tgttcaccag ggcgcgggcg
      121 atggcgacgc gctgacgctg gccgcccgag agctcgttcg gcttgtggtt catgcgtgtg
      181 tcgagactca ccgctgtgag ggcggctttc gcccgctgga tccgctcttc ggccggcgtg
      241 ccgttgtaga taagcggcag ctcaacgttg tgcagcgcag tggcgcgggc gagcagattg
      301 aaggtctgga agacgaatcc gatttcctta ttgcgaatgc gggccagttc atcgtcgttc
      361 agctcgctga cgagatgatc gttgagccag tatttgccgc tgctgggcgt atcgaggcag
      421 ccaatgaggt tcatcagtgt ggacttgccg gagcccgaag gtcccatgat ggcgacatac
      481 tcattccggc atatgtgcag gttgatgccc tgcagggcgt tgacctgctc cgagcccatg
      541 tcgtaggtct tccacagatc ttcgacgacg atcatgtcgg tcgcacagcc ggtgggttgg
      601 ggccgggcgt ggtcctgagt cgtggtttct gccgccatga gggctcctgt tgccatggta
      661 atggtatgcc gtgcgccgga ccgcggttct cgctgattag gaggaagacg acgagctgtc
      721 tttctgcggc gccgccaggg aactgtcctg tttaaccagg gagttctcct tgaggtcgcg
      781 cagcacacga taggtgccgg tgacgatgcg gtctcccgtc ttgaggccgc tggtgacttc
      841 gatatcggtg gcgccggtaa tgcccgtggt gaccggtacg aaatggacgc ggtccttacc
      901 gttcaccttg cttaccacga agacgccctg gacttcctgg gtcggctggg aattgttcgt
      961 tgcagccgaa accgttgacc cggagctggt gggggtcttg tcgttcagat gtttgggatc
     1021 gcgcatggtc agcgcctgga tgggaacggc gatgatgtcg tttttcttcg ccgtggtgat
     1081 cttggctgtg caggagagac cggggcgcag gtcattggaa ggctcgtcga gggtaacgac
     1141 caccttgaag tctttggcct cttcgacgcc ggtggtggac tggctggtgg actggccgct
     1201 ggagcggagc agggcctgat cgccaacctc ggtgacgtgg cctttgaaga tcttgccagg
     1261 aagggcatcg attgtaacgt cggccgtctg gccattccgg acgttgacga tgtcggtttc
     1321 gtcgaccttg acctcggcag tgatgacgga catgtccgcc agggtcatga gggtggagcc
     1381 ttcggcattc tgaatgccca tgacgaccat ttcgccctcg cgaatgggct tgttggtcac
     1441 gatgccgtcg aagggagcca cggcgatggt gcgatcgagc aggtcctgat tgacctgaag
     1501 tgtggcctgc tgggtctgca ggtggccgcg ggcggaagcc gtctgggcgc gcgcctgagc
     1561 caggccggct tcagcctgag ccagactggc cacgtccaga tcgtaggctg ccttcttggc
     1621 gtcgaagtcc tgcttcgaca tgacctgcgc gttgaaaaga gcctgagcac gctgatagtc
     1681 gagcttcttc tggtcgaggt cggctttggc gtgggcgacg ttggcctcag ccgtcttttc
     1741 tgcggcgata taggaattga tatctgtgcg ggccgcgtcg atgttggcct gctgcgcggc
     1801 aacgttggcg tgttgctgca cgttttcgat ggtggcgagg atctggcctt tcttcacgtg
     1861 gtcgccttcg ttcacgtaga agtgggtcac acggcccatg gcgtttgccc cgacattgac
     1921 atacaccttg ggcctgacct ggccggtggc actgacgatc gagacgaggt tttctttgga
     1981 gacggtaccg atgaggactt tggtatagcc cgactgagca cgaatgacag tgaagccgac
     2041 aattgccccg aaaaccacga cagcgccgat gataagaaga atctttttca atgcagtgtt
     2101 cctttgcgag cgtttacgca aaccctgggg aggcagttcc ccgggaacct caggcgacag
     2161 actcacaatg gtccgtctgc acggcaattc cccgggccgg aacgatcgat caggttccag
     2221 ggaatcggaa gcgcggggcc gacatgcgtt gccgaaagag atttccacgc catgataaca
     2281 ggttggcagg cagtttcgtc tcacccacac aggggtctgt cccgggggta cccgggcgcc
     2341 gttgagcagg ggaaatagct tgaaagggct ggttccggct cgtagaattg aaagcacccc
     2401 ccgaaggagt agttgccggc gtatgtcctg gagtcgattc tccctccctt ccgcgatttt
     2461 gatcactgtg ctggcaggcg ccggtcccgc tgttacccgc gcctggagcc aggatcagga
     2521 ctccgcacaa agccagcagg aacaacagga tcccctgaag cggccgttga gcgacaagca
     2581 acggaggcag cagcagaagg aactgcattc ggaactggag acggcctaca agaaatggct
     2641 ggaccaggac gtccgctgga tcatcacgga tcaggagcgg aaagcgttca tgagcctcag
     2701 caatgacgag gagcgcgacg cctttatcga gcagttctgg cggcggcgga atccgaaccc
     2761 ggattcgccg gagaacgagt atcgcgagga gcactatcgg cgcattgcct acgccaacga
     2821 gcactttgcc gcgggcaagc ccggctggat gacagaccgg ggcatgatct acatcaagtt
     2881 cgggaaagcg gactcggtcg attcccatcc gagcggcggg cagtacgaac ggcccattga
     2941 cgaaggagga ggcgagacct cgactttccc ctttgagacc tggcactacc gttatctggc
     3001 gggaatcgga gacaacatcg acatcgagtt tgtggattcg tgcatgtgcg gcgattacca
     3061 catgacgatt gaccgttcgg agaaagacgc tctcctgcac gtgcccaacg gtggccagac
     3121 gctgtacgag cagatgggga tggcgaagaa ggcggatcgc tttaagggcg gcctggagag
     3181 tttgggagaa gggccgggca cctcgggcaa tcagagcaag gagttcgatc gcattgagct
     3241 gtacgccaaa ctccaggccc cgccggaaat caagtttaag gacctgagcc tggcggagtt
     3301 cctgaccagc aagaaaatgc tgaccgggcc gttctttccg tttgaagtgc ggacggattt
     3361 tgtgaaggtg accgacgaca ccgttctggt gccgatcacg ctgcagatca aaaacaagga
     3421 catcaccttc agcaccaagg atggcgtatc gaaggggaat gtgagcatcg tcggccgaat
     3481 ttcgacgatc accgatcggg tagtccagag ctttgaggac cccgtggaag ccgggccgta
     3541 tccggcggag ctgctgccac gggaaatggc gggcgcgtcc ttgtactgga aggcgtttcc
     3601 cctgcggccg ggaaggtatc gcatcgacat tgccatcaag gacgtcaaca atcccgatca
     3661 cgtcggcgtg tgggcccagg cgatcacggt gccgcggtat gacgacgacc acctgtcgac
     3721 gtcctcgctg attctggccg acaagatgca gagggtgccg tcgaaggata tcggagcggg
     3781 caatttcatt atcggcaaca catttgtgcg gccgcgggta acgacgaatc cagcctcgcc
     3841 ggcgaccttc catcgaaacc agaatctgaa cttctggatg caggtctata atcttggcct
     3901 ggacgagaaa agcaagcaga acagcgccag gatcgtgtac cagattttga atgccacaac
     3961 gaacaagtcg attctggata cgacggaaga ttcgaagaag ctgagtccga attcggacca
     4021 gctgacgctg gagaagtccc tcccccttgc aagtcttgag cctgggaagt acatcgtaaa
     4081 gatcagtgtg gatgacgacg taacgcgaca gcagattgcg caatcggcgc catttacggt
     4141 ggaataggtg caggatggtt ttcgggcgcc cacacccgga acgaaaacgg cgcttcagtg
     4201 gtcgcagagc cacgaggcaa ggcaggattt agggagtagc gccaggagca agaacttact
     4261 gatttagtga gccgacgtcc caccatctgc gtccctctgt tgatcgcgct ggccgtgttg
     4321 tgcgccgatg cgcccagggc gagcgcgcag ggcaacagtc tgagcggcgt gattcgcgac
     4381 agccgcggtg cgccccagcc gggcgtggcg gtggagctgc tgcggccgga tctttcggta
     4441 gtcagcgaag tatttgcgga cgcacacgga cgctaccgca tcgagggcat cctccccggc
     4501 atttacgaaa tcaaggcctc aggcgcaatg tttctgccga cgctgcggga aaacctgcag
     4561 gtgctgacag ggacaaagct ggtggtgaac ctgacgctga acacgttgta cgaggctttt
     4621 cgctggctgc cggccaagcc gcgccaggcg gatgagccga aggatgactg gacctggacg
     4681 ctgcggcttt ccgccaatcg gccgctgctc cgcttgctgg aggacggccc gctcgtggtg
     4741 gtttcaaatg gcgacggttc ggcgccggaa ctgaaagcgc gcgtaacggt gcgtggcggc
     4801 gagagcgggt tcggcgacgg gggactgcac aacgacttcg aactggagcg taccccggag
     4861 gacggacggg cgatgatctt ccgtgccgac ctcagccacg gaagcccggg gcagctgatt
     4921 gatccggcgc tgaatgcgat ggtcggttat gagcagcagc tggcgctggg gcggacgttc
     4981 cgcgccgtgg gcgctgtgga gacgcggccc gatatcgcgg gcggacccct gggccagggg
     5041 ctgcaggcaa tagagctgcg cagcggccag accatgaatt tcggtccggg tgtccaggcg
     5101 gagtttggca acgagatgca ggggattcgg ctcggggata cgatcctcgc gaatcatcct
     5161 tttgcggccg tcacggtgca tcgtggcgcg gcatatgtgg gctatcggat gtcgacggcg
     5221 ccgggtatcc agacggttga ggatctggac cgcgaggcga cagtggagcc caggctgggc
     5281 gagcggaacg gcagactcac gctggagcag ggtctccacc aggagctggt agccggcaaa
     5341 ggcgaaggcg acactcgcgt gaagatggcg gtgtggcacg agcaggtgaa aaaccccgtg
     5401 gtgaccggcg gcgggaatat atccgcagcg gactgggcgg ggggcaacct gctgtatgac
     5461 gagacgagcg atctgctgcg cgcgacgggt cagagctatt ccggagaagg gatgctgacc
     5521 gaagtcgagc agcgcgtgaa cggcagcacc tggatctcgt ttgaagtggc tctgggagac
     5581 gccctggcca tggccgatgc ggcttcgggc acggatagcc tggatgaggc gctcaacggc
     5641 atgaaggccc gggagacgcc gatggtcggc gcagggattc gcggcaaggt ggcggctaca
     5701 gggacccagt ggcgggcgtc gtaccggtgg cagaatcctc gcacgctgac ggcagtggat
     5761 gcgttttcca gcggcgcttc gtcgccgtat ttgagcttcc tggtgcggca gccgattcgc
     5821 taccggcgga tgattcccaa tggagtcgag gcactggtgg atgtgcggaa tctgctggcg
     5881 gaagggtacc gccccttcgt ttcgagcgac ggaagcgtcc tgtattttgc agaggcggcg
     5941 cgctgcgtgc agggcggttt gtcgttcacc ttctgacgtg tgctctcagc agggccccgg
     6001 tcagtcagta cggttctcct ccataaagtc cagcaccggg tgaccggaac tgcagcaatc
     6061 gctgctcgtc tcgcttcgtt actgcagcat cgattcgacc agcagagtca ggccggtgat
     6121 gaagaacagg aacatggcgc cgatcatgac ctgagtccct ttcgatgcgc ctttcgtttc
     6181 cttccagacg cacacacccc agatggctgc aatcagcgtg gcgccctggc ccagaccgta
     6241 ggcgatggcc gggctggcgg ccttcgatgc gacgatgttc gaggtgacgc cgattccagc
     6301 gataaccccg cccagaacgc cgatgagatg gtacggcatc gtgccgcttt tgaagtagtc
     6361 ccggtaggtg accttctcgc cttttaccgg atgggccatg aagtacgtgt tgaagagaag
     6421 gttcgagacg cccgatccga acgcaaacac cacgaccgcg gcgtagggcc cgaatttgcc
     6481 aggattcagg tggatgaagt ccagcgaaac cgagcgctgg agaagaaggt agaagagtcc
     6541 catcaggacg ccgcctgaaa cggcgagcag aatccctttg cgggtttggc cgggcccgga
     6601 ggtcgggccc tggcaggcac ggtaggcgag tccgtccagg ataatggcga tcacgatcag
     6661 cgtgaccccg ccgaaaatga gcagcggatt gcctttcggc tgggcgacgt agttcaccag
     6721 cacaccctct accagcgcga tgccaatcgc gatggggaac gcgacggcca tcccggccag
     6781 atccagggcg gccatgaaca aaatgttcgc cagattgaag accacgcccc ccaacagcgc
     6841 ccaggcataa ctcgcgccgc tgccctgagc cagatcggcg aagaaggtcc tgccttcgct
     6901 gcccagcgag ccgaacgtca gcgccagaat cagcgtgagc aggaaatatc cgatggcctg
     6961 atcccagtag aagagcccga atccatagga gcgcggttcc atcttgagcg tattggccca
     7021 cgagccccag cagaacatgg tgaggatgca cagggccacc gcgacgggat acgagtgaac
     7081 gatgatcatg agatccagac tctcggggaa gaacgtgtga agtgtagaac acgaagctgc
     7141 agttgtcttc ggcccgcctg tcgtcgtatc cgctgatggc gattcaaccc gtctcctggc
     7201 ttatcgaatc gcagtgtcaa cgccggtgat ggattactat gcggagcaga acctgtttgc
     7261 ctttgcaaga cgtgttctcg ctcgacgccg gacgggtctc agccgaaccg aacaccgtcg
     7321 agtatggtgt gggcaaagga atccgtcggc atcgcctgcg attgctttgc agaaccgctg
     7381 attttcgcaa cctgtccgga gggacagttc atggctgcca ttacgcaaac cgcacctgcc
     7441 gctaccgcta cggcgcgcgt cctttcgctc gacatcgccc gcggcatcac catcgccttc
     7501 atgattatgg tgaacaataa cggcgcggcc gcgtaccatc ccttcaccca ctccgcgtgg
     7561 aatggctgga ccccgaccga cctggtcttt cccacttttg ttttcattgt gggagcgtcc
     7621 atcgtcctcg cgttcggcaa gcgcttcgaa agcggagcct cccgagcacg catcgcggtc
     7681 accatcgtgc gccgggcggt catcctcttt gcgcttgggc tggtcgtcaa tggttttcct
     7741 cattttccgc tgcacacgct gcgcatctac ggtgtcctgc aaagaattgc tctttgctat
     7801 ctaattgcgg ggctctttta cctgtggcgt cgcgacgccg caagtaagat cgtcgcgatc
     7861 gttgtcctgc tggtgggcta ctggatcctt atgcgctatg tgcccgtccc tggcttcggc
     7921 gtgccgacac atcagattcc gctgcttgac ccggaccgca actgggtcgc atggctcgac
     7981 cgcaaattgc tgccgggccg tctttatgaa ggcgttcgtg atcccgaagg cttgctgagc
     8041 acctttcccg ccattgctac agccctcctc ggcgtcctcg ccggtctgtg gatgaggaca
     8101 aagcgttcac gggaacagat cgccacgggc tttcttgcag cagccgttgt cctgatcgcg
     8161 ctcggagagc tttggagcct gacattcccg atcaacaaga aactgtggac cagctctttt
     8221 gtcctcttct gcgcgggttg ctccttgctg ttactcggca ttctctactg gtgcatcgat
     8281 gtgaagcagt ggcgcaggcc tttcacgcag ccatggatcg ttttcggaac aaacgccatc
     8341 ttcgcctata tgctttcgga actgctcgcg tcgacgctgt ggagcgtcca catacgtggc
     8401 ccggaaatgc tctggacctg ggtttacaca ccctttcatc acatccaccc gccgggcatc
     8461 ggttcactgc tctactccat cgcgttcacg gctttctgct ggctggcaac gtggccgctt
     8521 tggcgcaggc gcatcttcct gaagatctga aagatgggtc gcggtcatcg atccagttgg
     8581 tcatctcacg aatggtttca ggaagctcat tctcggaagt ccggtgcgct gcggaaccgt
     8641 cggcccaaat cggctgaact tcacagaacg gggctgcaat gccttcatcg atcgttgtcg
     8701 tcggcagtat caatctcgat cttgtctgca ccggagagcg aatccccgcg cctggcgaga
     8761 cgctgaaggg caatcggttc cagacgtttc acggtggcaa gggagccaat caggcagttg
     8821 ccgtggcgcg gctgggccat cccgtcaaca tcatcggcaa ggtcggcgat gatgacttcg
     8881 gcgaccgttt gcgccgcggc cttcgcgatg ctcatgtcgg tgtcaggaat gttttcacgg
     8941 tgagcggcgt ttcatccggg gtcgcgctga ttaccgtcga tgcggcaggc caaaacagca
     9001 tcaccgttat tccaggagcc aacggacaac tcctgccgcg ggatctggag aaaagcctgc
     9061 cccaactgcg ctcggccgcg atcattctta cgcagctcga gattccgatg gagaccgtgg
     9121 aatatctttg tgcgaccgcg cgccgtttca acatacccgt tgtcctcgat cccgcgccgg
     9181 caggtcccat cccgcgcagg atactgcgca atgtcgcctg gctcacgccg aacgaaaccg
     9241 aaacctgcgc gctgtgtgga atcgcccccc acgagctgac ttctgcaaac gtggattctg
     9301 tcgctgaact gcttctcgga aggggtccgc taaacgtgat tgtgaagatg ggtcgccgcg
     9361 gcgcgtatgt tgcgggcgcg gacggtctcc ggcggatgat tcctgcgttc aaggtcaacg
     9421 ccatcgattc caccgcggcc ggtgatgcct tcaacgccgg tctggccgtt ggactggtcc
     9481 ggggcatgcc gctggtcgac gccgctcgct atggtgctgc ggtcggagcc ctctcgacca
     9541 cgcgcgccgg ggcccaagcc tccatgccgt cggttaggga agtcagccgt cttttggagc
     9601 cggctcgtga aagacaccgg cgcactcgat aaatcgaagg tcagttttga atcccgcgct
     9661 gcggccattt gatgaaccgt ggccgcaaac accatcagca aacctggaca aacggaattt
     9721 ccagcagtcg ggccagcttg gcgatgagtc caccgaggtg accaataccg acggcgcagt
     9781 gatgtgcggg tccctgctgg ttccatcgct ccacaaacct gcgggcgccg atcggaaagc
     9841 ggtaccggct gttggtgttg ccgatcgcca gcgtggggcc cggttctgaa gatccatgcg
     9901 cggcaagcaa gcggaagccg tgctcgcggt catcgaccac cgacagcaga gtgaccggcc
     9961 cataccgaac cgacatctcg accgagagtc cccgaccgac tttgccgtga tagacctcga
    10021 ggggccgcac gcgtgtcttg ccctcggcga tggcgatgtg gcccggaccg tcgtgtccca
    10081 tgagcacgat atcgtccgcc agatccagtg cgtaatactc ggtgaacgag ccgcctgcgt
    10141 cgaacaggtc catgatcttc atcgcaatgg cgttcttcac ctcatactcg cccgccaccg
    10201 gaatatgacg ggcggtgagc agggaagtcc cgaggatcgt tgaactcatc gtgtcttcat
    10261 ttgcgctgac tcccgttcct ttgtagtagc aggcaagaga ctgaagatcg tgctgttcca
    10321 cgagtcgatc gagtgcgagc gaggtgcgcg ccgcacgttc cagttcggag cgtgcgcaac
    10381 ccggctgcag atcgaagtgc ttctcgaagt gccgcactcg atgcgcaatc tgatcctcgg
    10441 tgcaatcgtc tcgcagcgca ctcagctcgt ccacctcgag catctcgatg tgactgccga
    10501 acgtaatcgc gacctgggtg gtgtcggtgg ctacgtcgag catgccgcca tagtaatgcc
    10561 ccagcagtcc cagccgggtt tctgccagtg ctgctttgac ctgcgcggcc ctgatccact
    10621 cctcgatctc tttccaactt gtttcatccg tcaatgcccc ggttacctga tggaagggga
    10681 ttcctgctcg ccccagcaca ttggcgatct ccggcaccgg acacgccgag caccatgcca
    10741 gccacgcccc ggtcatcgcc gtgcggtcgg gcagacggtt aaaggcttcg tagtccagcg
    10801 cagcaccggg ctgcagattc agcacgatca cgggcacctg cgcgcgctgc accagcggca
    10861 acaccgtgct ggacaaggca tacgtcgtca catacagaaa aagcacgtcg atgtctgcgc
    10921 gccggcacgc atgtcccgcc tcacggctgc gctgcggcgt atccaccaga cccagagatt
    10981 cgacttgcgc cccgaagccc tgcagacgcg ctttcacctg gtccagatag ccttcgagtt
    11041 gttcacgcag tcctgcgaac tgcggccaat aggcatcgag accgatgccg cacaggcctg
    11101 cgcgcagtat tcgcgttgcc atcgcgcgcc tctattccgc cttcaaaacg gtcacggcaa
    11161 acggggccag cgtcagctgc ggaccggtca gggttccggt ccgcggcgga tcgtcgccgc
    11221 ttgcttcgtc caccgttgca aactgtgcgt gttcgaatcc cgcgggcacc gtcgccgtct
    11281 cctccgcgtt gcttcggttc acgagcagaa tcttccgtcc ctgtgcggtc gcgaagccct
    11341 gcacgtacgg ctgtggttgg tctccggaca gcggttctgt gtttacgaga cgatcgccgg
    11401 gatgaaaatt ctcgatgatc atcttcagca cccagcatcg cgggttcggt ttcccgttgt
    11461 tgtagtccac catcgacacg ctgggatact gtgatgggta tcccaccagc tgcgactcac
    11521 cgatcacatc cacgtccatc ttcgatagct ctgcaaacag gtaggcatac agcgccgctg
    11581 ccgcattcca gtagcgacgc ggaatatgat ccggcagcgg tttgccggcg cgaatctcta
    11641 ccccatcggt ggggagaata gcgcccaact cgtcggtgtc gatctttgtc tccggcgaca
    11701 agcggtcccg gatcgagaga atatagcgtg tcactgcgag aaatccgtcc gcctgattga
    11761 aaagcgtgtg ctgccaccca tcgggtcctt catcgagcgc cggtgttgca tagaagtgga
    11821 aagacacata atccagggga atgccgggac ggtgattctt cgcgttgaga aagtactcga
    11881 agtacttcgg attcgcgctc ggcgctgcca gagccagacc cacgaacttc gtctccgggc
    11941 tgactgtgtg aatcgcggag acgattgcgt cgtagcgttc tgtgtattgc tcgggcgtca
    12001 tctgatgctc gaaatcgacc tcgttcagaa cctcccaata cggaaacttg tagtgatagc
    12061 ccgagtcgtg gcgctttccg ttctcatcgc tgaaaccacc tttcgtgaac cagctcacca
    12121 ggcgcgcgta gtagtcgccc aactccttgc cgctcggatc gcgcagctcc gtgccctgtg
    12181 tgtagttcca gtacacctgg ttgggatcgt cgggatacgt cacgggtttc tctgtcttga
    12241 acatccacgc gggaattgtg ctgaagttga cgatcgttga gtgcccctcc gtcgccgcga
    12301 ggaagtcttt cgtgaccgga tcgatatatt ggaaatccca ggatgtgtgg tccgtggttg
    12361 gcggctgcag ctccgccact gcaatcctcg gataaggcag ccagggaacg aaacgaacat
    12421 aatcggcgcc gagcatcttc agcgctgcga atgcgccatc gtggatcggc gatcctggat
    12481 tgagcatggg gttggtcacc acctgcagcg tgggcgtcga cttcgaaacg atcaccgtct
    12541 tgtcccaatc gatcttgatc gtgccggatt tagccgctgt ttcctgggcc atgatggcgc
    12601 aggatccgaa acagacagcc gcagccacaa tcgacatccg gaattgcatc gctggtttcc
    12661 tccgccagac tgcccgagcc cacccgcgcg gacacgccag caataaatgt ccgggcactc
    12721 caggatcgcc cggcaagcag caccccagtc tacgcaaaga agcgcggtgt cgccatccgg
    12781 acggcaggca gccgtcggca aggctcgtat cgccgatcga cctggtccgg tgaccgacgc
    12841 tcctccgcgc accaggcagc caccgctttg ggcgccggaa aaggctgact tgatcagggg
    12901 gcggattgaa tcgggccgag gactgaattc ctgcaccgtc gatcaagggt cccgaggaga
    12961 ttgacggtga tcttgccgct tcttcgcttc ctggtttagc cttaagcgtt ctgagtgggt
    13021 cgatgccgct tatagaggcg tcaccgatta cggtgaaacc cgtttacaga tagcggcacg
    13081 caaccgccct tcggggggcc gatgaaagtg actccaggtt tggaacaccc tcaacccgag
    13141 gccagcggat ctgtgcgaga gggcggagag tcgcaagccg tcatcctcga tgaactgcag
    13201 aaaatactgg agagtccgtt cttccacaaa tcgcaccgca gcagtcagtt tctgcgcttt
    13261 gtggttgagc gcaagctgaa cggggatcca gaccagctga aagaacggac gatcggagta
    13321 gagctctttc accgttcccc tgactacgcc accggtgatg atgcggtagt gcgtgtccag
    13381 gctggggaag tacgccgcaa gctggatcag tactatcagg cagccagcga tgagggaatc
    13441 gaaccgctca tacgggttga actcccggtc ggatcgtatt cccctgtatt tcatctaaca
    13501 tcctccaaaa ctcgcgacgg attgtcagat tcagcgcctt cggccgacac cctgagcccg
    13561 gcaccggcga ccgcagttgc aaggcagctc tttacaaggc cctggtttct ggcgacaatc
    13621 tgtgtcgctt tgttgggaac ggtgtggtgg gtaacacgcc aggagcggca ggcaaagccg
    13681 gagccatcgt tgacgcgcag gttctgggag cctgtttttg gcacgcccca gccggttctc
    13741 atttgtctcg ccaagggtgt gacctatcgt cctgacgaaa aactgtacga tctctacgct
    13801 caaaatcatc cgggaacttt ccgcacagaa gtggagcgct caaacgatcc gttgccgctg
    13861 gatccccatc aatcgatctt gtggggtcag atgaatcttt atgacgacta cggaatagca
    13921 ggcggggatg tatcgagcgc attgcggatt tctgagtatc tgggcagcat cggtaagccg
    13981 cttcaggtgc ggatcggaag caactattct ttcgaagatc tcaggcattc tccagcagtc
    14041 gtgattgggg cctttaataa taagtggacg atggatctga ctgcgggcct tcattttgaa
    14101 tttgtggagc agccgggtag gactcttgcg attcgcgagt cagtccctgg gggccgagag
    14161 tggcacgtgc cggccgatcc cgccacagat tacggtatta tttccaggct cctcgactca
    14221 aaaaccgggc aattcatcgt caccgcggcc ggcctgacag gaagtgggac ggatgcggcc
    14281 ggagagctgg caacgcggca ggaatatctg gagcaggttt tgcgaagtgc accggctaac
    14341 tgggaaaggg gcaatgtcca gatcgtcttc cagacctcgg tcttggacca ggtgccaggt
    14401 ccaccacgcg tgatcgcgac atactactgg gaaagtccct gagcaaagaa cgaccggcta
    14461 tctcctggcc agttcgtttc taatcccact caagcgcttt acagtgtcgg atcagccggt
    14521 cgcttacttg ggagctgccg ttagcgtctt taagtgtgcc gggactgttg gtttgatttg
    14581 agcgcatggt tttccgctgc acaattcacc ggtaatccct tcgccctcag tgatcgtata
    14641 aatgcggtcc accgccggca gcctcacaga ttctttcttg ccggagggcc agtgaatttc
    14701 tgccgttccg gcatccgtgg agccgcccag cccaaaatgg gcccgcagat cgttcgaaga
    14761 caaatagctt cctccgctaa ggacatcgcc gcgttggcgc atgccggctg cgttcagata
    14821 cacagtagct cccacagcat cacgcgggct tcccggacct ccaatgagcc tgaactccac
    14881 ccagtgatgg tgatcagcgt tgacgttgcg aagcaacacc ggcaccccat ccatgctgtt
    14941 aatgactaca tcgatcctgc cgtcgttgaa gatgtccccg aaggcggctc cgcgccctac
    15001 cgtcaccaca gcgagccctg ttccctcaac ggcaggcacc aactcgaact tgccatcctt
    15061 cagattgtga tagagaagcg tgcgctgggc atagctttgt ccccactcgg gatgctcgtc
    15121 cacctgggga tagacatgtc cattgacgat gagcagatcc ttccacccgt cattgtcgta
    15181 gtccagaaat ccatcggcga aaccgacaaa gggaatggat gacatggcaa tcccgacttc
    15241 atggctgacg tcggtgaaga atcctgttcc atcgttgcgg aacagaacgt tgtaatcgtc
    15301 ggaaaagtcg gtgttgacga tggacaaatg tccgttgttt tcgtagtcgc cggcggcaat
    15361 gcccatgtta gcgatttctc gtccgtcttt gttcagaccg tatccgctga tgtagctctc
    15421 gtcctcgaat ttgccattcc ctttgttgat gtagagatag ttgggcgttg aatcatttgc
    15481 gaccatcaga tccggcttgc catcgttgtt gacgtcagca aacaaaacgc cgagtccgta
    15541 gtatccgcgc gaatcgtcga cgccgagttt tgcgctcaca tcggtaaatg ttccgtctcc
    15601 gttgttatga aatagatggt cctgttctcc ctgcaggccc cgcggaccgc acatcacctc
    15661 tacgccgcgg tattgacaaa tcgcataagc gatccccttc gaccctgaaa ccggcggatt
    15721 ggcgaggtcg attttggcat acccgtcaac gaacaggtcc aggcgtccat cgccatcgta
    15781 gtcgccgaag gtcgcgcccg cggaccaggc accgatcgtc accccggcct tgtcagcgta
    15841 atcgctgaag gttccatcgt ggttattgtg atacaaccgg ttcttaccat agttagtgac
    15901 atagaggtcg ggccagccat cattgtcata atccccgaca gcgcagccgt atccccagcg
    15961 gtcgttggtt gctcctgcct tctccgtcac gtccgaaaag gttccgtcgt ggttattgcg
    16021 gaagagggct gcatgcggcg gtgccgcttt gccggccaga gcatcgtagt tcgagccatt
    16081 gaccagatag atgtcgagcc agccgtcatt gtcaaagtcg agcaggcaca ctcccggccc
    16141 tttggcctca aggatcaggc gcttttccgg cgtgcctgca ctgtgacgcc aggtcgtcag
    16201 gccggcggct ttggcaacat cctgaaagac gatcggaccg gttttgacga agcctccagc
    16261 ggtgatgggg cgatgctggg tgtcaaactg ggcctcctga ggtccggcgt tggccataac
    16321 cccgcccggt ggcttttgcg cagtttcttg ccggccggct ggaatatttt ccggagtttg
    16381 cgcagcctgg gcccgcatgt ctgtgcctgt ccccagggcc aataaacagg cggagaagcc
    16441 caacaccgcc gcaaggcagt ggcgaattgg gttcgccgat ctgcccactg cccgtgtggg
    16501 gcgggatgca ctcgcagcaa cagataagct catcgtcatc ggtgcgtcag tgttgtccat
    16561 cggaatcagc agccggcgat ccttcctcga cgagccggta gcgcttgcga tcggtttcac
    16621 gctgcaggga ctccttgtgt atctcggcga gccgctgaac cagaggctgt aactcctcgg
    16681 gagtatggcc tgcgtgtcgc atggacataa ccaggtggta cattgcggct tcgttggagg
    16741 gatcatattg cagggcggtc ctgcactctt caatagcctt ttgatagtca ttcaggctca
    16801 tatacatgcc cgcgagctga ttgtgtgcct cgacaagatc gggctttgtt ttggcggcgg
    16861 ccagagcgtt ctgcatcgcc tcgttgaaat cggcgctccc aggcgcaggc gccctggtca
    16921 aaatcaactg cgccaccatg aggtgaaaca gtggattgtt gggatgagcc ctcaactgct
    16981 cgcgcacgcg ttcgaaagct ttctggggat cattccgctc gatcatcgtg aggtccccgg
    17041 cgtaagcagc aatgtgctgt gtggtgtcga tctgttccgc catcctgaaa tcagcttcgg
    17101 cctcgtcaaa cttgccgagt tgttcaaaca tgacgcctcg ggaaagatac agggcagagt
    17161 tatcggagat gcgcttgagc ccggcattga gcatatcgat gcctacctga aaagaatcgt
    17221 gcgtcagaca aaggaccgcg aaaaggacgt agttcgaggc ctcggtggga ttgaggacga
    17281 tggcctgacg ctgcagcgcg acagcgcgcg gagtattgcc gatggcttca taggcctggg
    17341 aggcgaggct gaggatgtca ggatcagttt ggccaggagt gagaaacggt tcaaggacct
    17401 ttaccgcgtc atcattctga tgcgcggaaa cgagaagcac cgcgaggttg taggagggat
    17461 aggcgctccc gggcaaaagc ggcacaagct ggcgaaaaac ggctattgcc tcgggcgtcc
    17521 tgtcgagttg aaaaaggcaa tagccataag cctcgagcga agccggatgg gtggcgattg
    17581 cagccctgct catctcgaaa tgagatacag cgtcgcggca gtcgccgttg tgctgttgca
    17641 gcgtggcgag catctcctgg gcagtggtgt cgccagggtc agacttgagc atcctctcaa
    17701 gcagaggaat agcccgcttg tcgccgctct tgtacaggat ttgggtttct cccctaaggg
    17761 cggcagtgta agtaggctgg atgtggattg catgatcgaa tgcggcgagg gcatccctgt
    17821 cgttcccctg cagggcaaag cagattccct gcagggtcca gagcctgtag ttctccggat
    17881 ttttgcggat ggcaatcttc aatgcagcga gcgcctgatc atagtgctgc gaacggatca
    17941 aagactcaac agaggagatc gaggtcgtcg ccgtttgggc catcgatgac gttgttaagg
    18001 ccaaagccaa gcccgcgatc agtctgaagc gaagcatatt gtgtttaact ccggggcgtc
    18061 cgccgatctg ccgtaagggc ggccgctgtt tctttcattc gatctctgcg cgcagcgcag
    18121 gccgcctcac ctggattcga ggaagaggat ccggctcagg agcagggctg tttcgcgcac
    18181 cataccccgg taatcccttt cccctcggtc acggtaaaaa tgcgatcgac agctggaagt
    18241 ctgactttct cgatactgcc ggaaggccag tgaatctcgg cggttccggc atcggttgca
    18301 ttggcaagtc cgaagtgagg ccgctggtcg ttcgaagaaa tgtagcttcc tccgctcatt
    18361 acgtcctccc gcaggcgcat tccgttcgcc gtgagataga cggtggttcc gatggcatcg
    18421 cggggactcc ttgcaccacc catgagcctg agttccaccc agtgatgatg gtccgggtta
    18481 acatttttca gcagaaccgg cggtctgtcg accgagttga taacaacatc gattttgccg
    18541 ttgttgaaca agtcgccgaa gacggcgccg cgtcctgtcg tgacgacggc gagccccgat
    18601 cctcggaccg gcggcacata gtcaaatcgg ccatgcccgt tattgtgaaa cagcaggggg
    18661 cgctcggcgt aagttgtacc ccagtcatgt tcgtcgacct gagggtagac atgaccgttg
    18721 atcatcatga gatctttcca gccgtcattg tcgaagtcga tcataccgtc gccccaaccg
    18781 acgaaaggaa ctgcaatttg agcaatgccg gattctcgcg atacatcagt gaaactggca
    18841 tttccgtcat tccggaacag cgccttgtaa tcgtctgaga aatctgtatc gaggatgtcg
    18901 acccggccgt tgttcaggta atcgccggca gcaagtccca tcgaggcaat ttcgcggccg
    18961 tcgaagttga aggcaaaacc cgatacgtaa ctctgatctt caaacttgcc attaccttta
    19021 ttgatgtaca ggtagttgcg ctgtgaatcg tttgcgacca gcaggtcgac cttgccgtcg
    19081 tcgttgacgt cgacgaagac ggcggtgaac ccatagaacc ggtccgggtc ggagacaccc
    19141 gcgaattcgc ttacgtcggt gaaggtcccg tcgccattgt tgtgaaacag gtgatccggc
    19201 tcgcccggca gccctcttgg cccacacatg accggggcgc cgcgcatctg gcagttagcg
    19261 gtggaggctc ttccgtcctc tgaactggcc tgatggtcca ggtcataatg caggtatccg
    19321 gaaacgaaca ggtcgagccg gccgtcgccg tcatagtctc cccaggccgc tcctttcgac
    19381 cagttgccga gggtcactcc ggctttctcc gctacgtcgg tgaatgtccc atcgtggttg
    19441 ttgtggtaga gacgattctt gccaaaattg gccacgaaga tgtcaggcca gccatcattg
    19501 tcgaaatccc cgacggccac gccaaatccc cagcgatcgt tcgtcacccc agccccggcc
    19561 gcaacgttgg taaaagtgcc gtcgtgattg ttatgaaaga gagccgcatg cggaggctca
    19621 cgttttccgg ccatcgcatc gtaagttgag ccgttcacaa gatagatatc aagccagccg
    19681 tcattgtcgt aatcgatcag agcaagacca gagccgtcgg tctcgaggat gtatttcttt
    19741 tgtggagtgc ccatttcatg acgccaggcg gagagtcccg cgggcttcgt cgcgtcctcg
    19801 aatacgacgg tgcccgtatc aacgaatccg cccgcggtga tgggtcgccg gtgttcatcg
    19861 taaacgggag caaagatgcc ggccgatgct atgccgcccc ccatcccgcc ctggaccggc
    19921 tgctgagtct ggcagcacgc gggcgcggcc agtgacaaga gcagggcacc gcgaaccaaa
    19981 acgcggacgt gcggcagaac ttcttccacg gctgttccga tgaacatctt atgtcagcca
    20041 ggctttcgat tctgaatttt ctttgcttgt cttaccaggc gtatgtcgcg atgacctggg
    20101 gaggcccggc caccgactcg gtgaccgtag tatgcaacac gatttccaga ttgcgatcgg
    20161 gccattcagt cggggctttg gccagtcctt catcaagatc atgcgcggag cccacaaatt
    20221 cgccggcagc ctgggtgcca cggggcccga ttcccgcgac ggtgaccgtg aactggccag
    20281 ttttggaatc aatgaggcgg ctgacaatcg cgaaatcttc caggacttcg ccatgagctc
    20341 cgacccgggt cgtccatacc cttccccctg ggacctgctc gcggatcatg tactgccccc
    20401 gatcccggac gaaggtgaag cgcaggttcg acagcaactg catggtccat ctgttgttga
    20461 acccgccgat gatgacggcg gggaaactgc gaaggtcctc ataagtgatc ctggcgccaa
    20521 tgcgcagttg gctcggccga tcaagctttc cgaacaaacc agacaggtcg gccgcaaccg
    20581 aggcatcgcc ggcgggaaca atctcgggat catcctgtcc ggcagaggtc ggggtaacag
    20641 ccgcttccac ttctctttgc gacgcgtctg ttcggccgtt ggaaggggca gccgcttcag
    20701 acccattctt tgagacgttg gccacacaaa tgagcacagg ctggtgactt gcggttgccg
    20761 ggccccagaa ttggtcgaac gccgtcctcg ggctgtcaac ccggcgcaaa gagttcagga
    20821 cggcgcggcc cagcaacgcg gcgagcatca cgaacaaaag gaagagagct accctgacaa
    20881 ctggtttctt cagatacggg cgatccctcc gagtcgcaga ttgaacttct gacttgtgat
    20941 cgcgggcaac agtgcatgaa tgaaagacag ggctatacga accaaccggc agttcaatcc
    21001 gcacccgggg cgactctggt gtctcctggt agtactggtc gagacggcgg cggacttcgc
    21061 cagcctggac tctcacaaca ggatcttcgc ccgttgggta tccgggaggg cgtttgaaga
    21121 cgtcggttcc gatggtgcgt tccttcaagc gttccgactg gccgtcgagc ttattcagaa
    21181 ccacgtaacg aagaaattct ctgctgcgtc tcgcactcct gaagtgctgg ctggacaaga
    21241 cattgtccag ctccccgagc acagcagagc ggtcgcattt cagtttgtcg gctgcgtcag
    21301 catcgcggtt tcccggcact tcaagaacgg aattcatgaa tctacccgcg gcttccactt
    21361 ccacgccctc cacatccctt cgggcgagta acgggcacgt cagggcctgt catcggggag
    21421 gggtgaaggg gaaagtaagc gttttcaggc ggacctagta gaccaagggc agcgaccgtt
    21481 gtcaagaacc gcatcggcag gagttcggct gagcatgtta ccaacgttca gacgtagtct
    21541 gcgggcgctt cctgtgcact tcccgtcaca aggctgtgaa ggtgcaagtg tctatgatgc
    21601 ttagggttaa ccctaaaatc taacagtaaa aataccgtgt tttacttgcg tttgccggca
    21661 gttgaggatc aaacttctgg ccgatctgcc aatacagctt ttgatggggc cacacgcatt
    21721 ggccgagatg cactacggga ggccatgagt ctatgaaacg ttggacaagc atttcgattt
    21781 tcttcgttct tttgtcattc attttcggag gatcaaccgc cctcgctcag ttctccagcg
    21841 gtgttcaggg cgtcgtacac gacacgagcg gggccgttat cgccaacgcg aaagtgatcg
    21901 tgaccgacac tcggctcggc gtctccagga ctgcccaatc gggggaggat ggctactttc
    21961 gtatcgatgg catcgctgcc tcaacctact ccgtcgagat ccagatgtcc ggcttccaga
    22021 cgtggaagca ggatggactg gcattgcaga tcgccgaagt ccgcaccatt gctccggaac
    22081 tgaaggttgg tgcggtgtcg accagtgtgg aggtctctgc atctgcagct tcggtcgacc
    22141 tggaaacgcc aacgacaggc gcgatcatct ccaatgagac tctgcagcaa acgccgctcc
    22201 ccggacagaa catttacggt ttggcatcgc tgtcgcccgg catgaccggc ggcggcatcg
    22261 tcaccggcgg caacgacaat tacaccaacg agtacgggat caacctcaat gcagcaggac
    22321 tgcgtcagga agagaacggt ttccagattg acgacggcta caccaatacg ccgtcccgcg
    22381 gcgggcagac atcgatctct cccaatcctg agatcgtcga ctccgtgaac gtccgtacca
    22441 atgatttcga tgcccagaag ggaaggaacg ccggcgcgac cgtggacgtg tacaccaagt
    22501 ccggctccaa tgacctgcac gggaccgtcg actactactt cctgaacgac actctcagcg
    22561 caaagacgca cttccagagt aaggttccca ccttcacgcg caatgaagta gggggtaccc
    22621 tgggtgggcc agccatcaag aacaagctct tctggttcgg tgccatcgat gttctgcggt
    22681 cgagtaatcc caacagctac acggccacag tggaaacgca ggacttctac aactgggcca
    22741 aggcaaatct gccgaatacg tttggcacac agatcctcgg catggcgcct ccacaaactt
    22801 atccgacaac cggtctggaa accgtagcgg cctatgaagc gacgtccggc acgccgggtt
    22861 attacgcgcc tccggcgggt atcccggcca cgatgaatat catcggtacg attcccgtga
    22921 gctacagttc gacgaagaac ggataccagt ggagcgtccg cggagactac tacatcacaa
    22981 acagcgatcg gctctacgtc gatgtgattc gcaccaagta tgacagcccc ggaggaggaa
    23041 cccggccagc cagtgttcag ggcaattcca acagctccga ttttttcaat gccaactgga
    23101 cccacacttt tagttcgcgt ctcattaatc aaacaggact gaatctgatc cggccttacg
    23161 gatcgaatat cacccaggtg gcgacggagg gaattcctta cgtcaatgtg aacaatctgg
    23221 caggatttgg ttcctgggcg ccgggtaatt tcacacaaag cacctatagc tggcgcgatg
    23281 tgatgacggc cacgatccgg aagcacacac tcaaggttgg cgtcgatttg ttcaataccc
    23341 gcgaggtgga tcaccagcag ggcgctttta cccggcccac attcaatttc cgtaacctgc
    23401 tcgatttagt tcaggacgaa cctgtctatc agtccggtcc gaacgtaaac ctgcagaacc
    23461 accagcaagc tccctatgat cgcgactatc gcgacctgat tcagggctat tacgcccagg
    23521 acagctggaa aatgaccccg cgattcaccc tgactgcggg tcttcgctac gacggaatgg
    23581 tgaacttctt cagcattaac agaccgatcc tgtccaaatt caatctcggc tccggctcga
    23641 cctggaacgc tcaggtagcc tccggtatcg ccgccctggc tcccaacaac cacgtcctcg
    23701 atcacaacat cgggggcttt accccgcgtc tcggtttctc atgggatccg ttcggcacgg
    23761 gcaagacggc gttgcgcggt ggttttggga tgttcgagga tcagcccccc tacctgcaca
    23821 tcactgactc aactgcggcg aacctgccaa actacttcaa tccgatcgcc gatgtcactg
    23881 ccggtagccc tactccaaaa ccgttcctgt gcacgccggc cggggcatgg aacatgaatt
    23941 gccctgttct tgataccagc aatgccaccg tcgatccgac caccggagcc cttcgcatca
    24001 atggagttgt tcagcgggcg gggatgggtg gctatgatcc caacttcaag atgcagcagg
    24061 tgattgcgtg gtcgctcagc gtgcaacaac aactgcagag caatctgatc gctgaactga
    24121 actattccgg cacggaaggg catcacctgc tggtcttcaa caacgacatc aaccggttcg
    24181 ccggcgacct gatccagaac aacaacaccc tgacgcgctt gaatccgtac ttcgcgggga
    24241 ctgcatatgc tacttccaac ggcaactcct ccgggcaata tggctccctg gcacttactc
    24301 ggcgatatac ccgaggcttt gctctgcggg gcatttacac ctatggaaag gtgctggacg
    24361 agatgagctt tgctcaatct
//
LOCUS       DASZYN010000590         8176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_405974, whole genome shotgun sequence.
ACCESSION   DASZYN010000590 DASZYN010000000
VERSION     DASZYN010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8176
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405974"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /gene="hemA"
                     /locus_tag="VHE33_15320"
     CDS             complement(<1..1269)
                     /gene="hemA"
                     /locus_tag="VHE33_15320"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HVT98874.1"
                     /translation="MRFQVAGLNHKTAPVELRERLAIGGEALPETTRALLGRPGVQEA
                     MILSTCNRVELLVSHNGREPQLPEFLAEHFSVDRGLLEPHLYRYQDEEAVRHMFRVAA
                     SLDSMVVGEAQILGQVKQSWNLARELGAVRGPLDKLLQSAFTAAKRVRSETEIGSSSV
                     SIASVAVDLARRIFGSLEGKKILIVGAGKMSELAARHLMQHGADSILVANRTYDRALR
                     LAEQFLGVAIPFEDLHARAHEADIVITSTGAGHAILGVDQAQAILHRRKGQPVFVIDI
                     AVPRDVDPKVNRLEGMFLYDIDDLQQVAASNLVDRGHEAQKAEAIIAEETERFRRRIR
                     TIDIAPVLVDVQGTAEELRQSELHRQRAWLQSLSREQQEAVEALTRGLMNKFMHMPLQ
                     ALKAAAREGDAAALEAIRAMFGREYGHKGPG"
     gene            complement(1266..2132)
                     /gene="ccsA"
                     /locus_tag="VHE33_15325"
     CDS             complement(1266..2132)
                     /gene="ccsA"
                     /locus_tag="VHE33_15325"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HVT98875.1"
                     /translation="MFLLWLRIAAGLYGVASILAIPAVLGNHPRGHRYCLPVAMGGWF
                     FHVVATVEMLATAHHLIPVLGTEIVSTLALAIVGVFLFTFFVYRTVSFGLFALPLAFL
                     LTVIPAIGPERYTFSSPMVRNGWIAFHVVMLLVAYAALAFSILSSFLYLIQEKRLKNK
                     DWAKAGVTARPKTKPRGFLTWLPPLATMDQVTQSTLQIGFVCMTVGLFAGSVVAQEQV
                     GVGYFADPKVLLSFLMWGLYVVILFVRRSVGMRGRRAAWITSSVFVVMLCVWAANKLS
                     GVHSSAHGFGLK"
     gene            3046..5463
                     /locus_tag="VHE33_15330"
     CDS             3046..5463
                     /locus_tag="VHE33_15330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT98876.1"
                     /translation="MTSDLKVALRQLRKTPGFTVTAILTLALGIGANAIVFSVLNAIV
                     LKPVKVPHPENLFMVQRMRFPSQSVPDYEDLRDRNRTFQSMTMYNIIGPVGFDTGNNP
                     ITAWPYEASGNYFDALGIQPYIGRFFHKDDEKGQNSAPFIVLSYDFWHTHFHDDRAAV
                     GRVVQINKHPFTIIGVAPSDFRGTEMFFAPAFWFPLVDANEVTGWDSLHARGSHSGFV
                     AGRLKPGVTPAEAKADLQSLASWLAKTYPKSDDGITFSLARPGLVGDMLGGPARAFVA
                     GLMLLAGLILLAACANLGSLFAARAADRAKEIALRVALGSRRGRIVRQLLTEAVAVSL
                     AGGALGLAGGVLILRWLSAWQPIPTVPINVPVNPDLATYGVALAMALVSGLLFGMVPV
                     RQVMKADPWQIIRTAGAGLGGIRKFTLRDLLLVAQIAICAVLVTSSLVAVRGLMRSLH
                     SNFGFDPNHVVVVATDLQMAGYKEADRAPMERRMVEAIQSIPGVSAAGYSNQLPLSLG
                     GSDSSVYKDTTTDFRPTNAAADAMNYSISPGYLPAAGTTLLAGRNLTLHDDDKAPIAA
                     LINREFAVKVFGSVNGALGGHFKYWGGKRAEVVGVVENGKYRTLTEDQQPAMFFSFLQ
                     QPDSGAWLEVRSMQDPATTSRAVEETLHRLDPGLPLQMSTWNQEMSSALFAARVATVA
                     LGVLGLLGAMLAVTGIFGMASYVVTKRMRELGIRVALGARQKQVLKAALGRAFILLAS
                     GSVAGIVLGVLATKLLSYIVYQATPKDPFVLGGVVLTMMLLGLLAAWLPAQKALAVDP
                     MILLRDE"
     gene            5637..>8176
                     /locus_tag="VHE33_15335"
     CDS             5637..>8176
                     /locus_tag="VHE33_15335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT98877.1"
                     /translation="MLPDLWHAFRQLRKSPGFTLTAVLTLALGVGATTAIFTLVQQVM
                     LQSLPVTKPEQLWRIGDQPRCCNWGGYSQSGADEPNDWSLFPWEAYRLFRANTPGFQD
                     LAALQAGNAALGVRPAGSQAPPDTANGQYVSGNFFRTLGVSAWRGRLLTDADDQQGAP
                     PVTVLSFHAWVQKFGSSPSVVGSVYQINGHPFTIVGVAAPSFIGAKMTSYGMPDLWLP
                     LTAEPIIDGATTRLKNPNVDWLDVIGRVRPGTSPSALQAQLQAELHGWLGSHLADMGP
                     TEKPLFEKQSLRLTPGGAGFSSMRRDYGQGLLLLMITAGCVLLVACANIANLLLARGL
                     RNRQQTAVRVALGATRGRLVRKALLESISLAFIGAAAGVAVSWAGARLILYLAFHTQA
                     NRTWIPVHASPSASVLLFALGLSVLTGALFGMAPAWMTSRAEPIEALRGASRSVGGGR
                     HWAQKTLVIAQAAVSLVLLSAAAMLGGSLRNLVHQDFGFTPQGRVLVSINPSLSNYKQ
                     AQLVPLFREIQERLSAIPGVRSVSAATYAPLSGDQWGHGIRVEGHPDPGPEDNTMADW
                     TRITPGFFDTIGTPMLEGRSITEADNTTTRPVAVVNEAFAKKFFGNQNPIGQHFGPQV
                     RKNAATYEIVGVVHNIHYVSYDFRAPARAMYYIAEAQPVHFDEPDTQTYELWSQNLYN
                     IVLWAPGHPSSLAAQVRSALADVDPTLVMYGVEPYPEVMAGAFDQQNMIASLTWLFGA
                     VGLLLAAIGLYGVTAYGVEQQTSEIGVRMALGADRRSVIAMILRGAFWQVGIGLGIGI
                     PAAIGTGYLIASQLFGVSPWNPLLLAGATALLGLAALVAALIPAQRAA"
BASE COUNT         1553 a         3044 c         2131 g         1448 t
ORIGIN      
        1 acccgggccc ttgtgcccgt actcccgccc gaacatcgcg cggatcgcct ccagcgctgc
       61 cgcatcgccc tcacgcgccg ccgccttcag cgcctgcagc ggcatgtgca tgaacttgtt
      121 catcagcccg cgcgtcagcg cctccaccgc ctcctgctgc tcccggctca gcgattgcag
      181 ccacgcacgc tgccgatgca gctccgactg acgcagctcc tccgccgtcc cctgcacatc
      241 caccagcacc ggcgcaatgt cgatcgtccg aatccgccgc cggaatcgct ccgtctcttc
      301 cgcgatgatc gcttcggcct tctgcgcctc gtgcccgcga tccaccaggt tcgacgccgc
      361 cacctgctgc agatcgtcga tgtcgtacag gaacatccct tccagacgat tcactttcgg
      421 atccacatcc cgcggcaccg caatatcgat cacaaacacc ggctgtccct tgcgccgatg
      481 caggatcgcc tgcgcctgat ccacacccag aatcgcgtgt cccgcgcccg tcgacgtaat
      541 cacgatatcc gcctcgtgcg cccgcgcatg cagatcctcg aacggaatag caacgccaag
      601 gaactgctcc gccagccgca gcgcgcggtc atacgtccgg ttcgctacca gaatcgaatc
      661 cgccccatgc tgcatcaggt gccgcgccgc cagctcgctc atcttcccgg cgcccacaat
      721 cagaatcttc ttgccttcga gcgatccgaa aatccgtcgc gccagatcca ccgccaccga
      781 cgcaatcgac accgacgacg atccaatctc cgtctccgac cgcacccgct tcgccgccgt
      841 gaacgcgctc tgcagcagct tatccagcgg accccgcacc gcccccagct cccgcgcaag
      901 gttccacgac tgcttcacct gccccaggat ctgcgcctcg cccaccacca tcgagtccag
      961 actcgccgcc acccggaaca tgtgccgcac cgcctcttca tcctgatagc gatacaagtg
     1021 cggttccagc aagccgcgat ccaccgaaaa atgctccgcc aggaactccg gcagttgcgg
     1081 ctcgcgcccg ttgtgcgaca ccagcagctc cacccggttg cacgtcgaca ggatcatcgc
     1141 ttcctgcacg cccggccgcc ccagcagcgc gcgcgttgtc tccggcaacg cttccccgcc
     1201 aatcgccagc cgttcccgca gctccaccgg cgccgttttg tggttcagac ctgcgacctg
     1261 gaatctcatt tcagtccaaa cccgtgggcg gaactatgca ctccgctcag cttgttcgct
     1321 gcccaaacgc acagcatcac cacaaacaca ctcgatgtga tccacgccgc acgccgcccg
     1381 cgcatcccta cgctccgccg cacaaacaga atcactacat acagccccca cattaaaaac
     1441 gaaagcaaga ccttcggatc ggcaaagtat ccgaccccta cctgctcctg cgccaccacc
     1501 gatcccgcaa ataaacccac cgtcatgcag acaaaaccga tctgcagcgt gctctgggtc
     1561 acctgatcca tcgtggccag cggcggcagc cacgtcagaa acccgcgtgg cttcgttttc
     1621 ggcctcgccg ttacccccgc cttcgcccag tccttgttct tcagccgctt ctcctggatc
     1681 agatacagga aactcgacag gatgctgaac gccagcgccg catacgccac cagcagcatc
     1741 accacatgga acgcgatcca cccattgcgc accatcggcg agctgaacgt gtaccgctcc
     1801 ggaccgatcg ccggaatcac cgtcagcaga aacgccagcg gcagcgcaaa taacccaaac
     1861 gaaaccgtcc gataaacgaa gaacgtaaac aggaacacgc cgacaatggc cagcgccagc
     1921 gtcgaaacga tctccgtccc cagcactgga atcaggtgat gcgccgttgc cagcatctcc
     1981 acggtcgcca ccacgtggaa gaaccatccc cccatcgcca ccggcaggca ataccgatgt
     2041 cctctcggat ggttcccgag caccgccgga atcgccagga tgctggcgac cccgtacaga
     2101 cctgccgcga tgcgtaacca aagaaggaac atggggaatt ctcagcggag agcgcgaaac
     2161 cgggggtatg aaaagtataa agaacccggg ccttcgagcg ctccgaaccc cattatccga
     2221 atcgcaccca gcccggaatg tgagctaaat cacacccccg aaagaagccc acccgctcgc
     2281 gcaccgccag gttcacccca cagcaagcag ctcaacccat ggcgtcgcga ttttcaaccc
     2341 ttttggggtt ttacccaagc cgaaagtacc gccgccggtt cccttctgga acccaacccc
     2401 actcggcatc ccaacccctc gcgtaccgcc aggttcaccc cacagcaagc agaagactcc
     2461 ctcattccgc acgagtcaac cgattggatc tccccaaggc caccaaaagt acctgcccgg
     2521 ttcccatcca gggaaagaag aaacgccgca ccgccaggct tctccccgct cagttccaat
     2581 tcgggaaagg aaaaccagcg caccgccagg ttcaccccac agcaagcaga agactccctc
     2641 attccgcacg tgtcaatcga taggctctcc ccaccacccc caaaagtacc cgcaccgttc
     2701 ccatcccgga aaagaagaac cgccgcaccg ccaggcttct ccccgctcag tttcaattcg
     2761 ggaaaagaaa gccatcgcac cgccaggttc accccacagc aagcagaaga ctcttccggc
     2821 tccaaaaaat caaagcattc cacccctcag accgccccaa agtaccgcgc ctcaagcccg
     2881 cattccaatt ctggaaatcc cgcgaagcct tctccccgcc tgcgcccaac cctcgagcgc
     2941 caccaaccct cgcccgcatc ctcaccaggg agtccaaccc aaaatccccg attccccgga
     3001 acggaaacga catgacttac gtaccgtgtc gcaaggacaa aacggatgac atctgacctg
     3061 aaggttgctt tgcgccagtt gcggaagacc ccgggcttca ccgtgaccgc gattctcacc
     3121 ctcgccctcg gcatcggcgc caacgccatc gtgttcagcg tgctgaacgc catcgtcctg
     3181 aagcccgtga aggtccccca tccggaaaac ctgttcatgg tccagcgcat gagatttccg
     3241 tcgcagtccg tacccgacta cgaagatctc cgcgacagga accgcacctt ccagagcatg
     3301 accatgtaca acatcatcgg cccggtcgga ttcgacaccg gaaacaaccc gatcacagca
     3361 tggccgtacg aagccagtgg caattacttc gacgcgctgg gaatccagcc atacatcggc
     3421 cgcttcttcc acaaagacga tgaaaaagga cagaacagcg cccccttcat cgtgctgagc
     3481 tacgacttct ggcacacgca cttccacgac gaccgcgccg ctgtaggccg agtcgtgcag
     3541 atcaacaaac accccttcac catcatcggc gtcgccccat ccgattttcg cggcacggaa
     3601 atgttcttcg cacctgcctt ctggtttccc ctcgtggacg ccaacgaggt caccggttgg
     3661 gactcgctgc atgcgcgtgg ctcccattca ggatttgtcg cgggccggct gaagccaggc
     3721 gtcacgcctg ccgaagccaa agcggatctg cagtccctcg cctcgtggct cgcgaaaacc
     3781 tatcccaagt ccgatgacgg catcaccttc agtctcgccc gccccggcct ggtcggcgac
     3841 atgctcggcg ggcccgcccg ggcctttgtc gcgggtctta tgctgctcgc cgggctgatc
     3901 ctgctcgccg cctgcgccaa cctcggcagt ctttttgccg cgcgcgccgc cgaccgggcc
     3961 aaagagatcg ccttacgcgt cgccctcggc tcgcgtcgcg ggcgaatcgt gcggcaattg
     4021 ctcaccgagg ccgtcgccgt ctctctggcg ggtggtgcgc ttggactggc cggcggcgtc
     4081 ctgatcctcc gctggctcag cgcgtggcag cccattccca ccgtgcccat caacgtcccg
     4141 gtcaatcctg acctcgcaac ctatggcgtg gccctcgcca tggctctggt cagcggtctg
     4201 ctcttcggca tggtgccggt ccgccaggtc atgaaggccg atccatggca gatcatccgc
     4261 accgccggcg ccggtctcgg cggtattcgc aagttcaccc tgcgcgatct gctgctggtg
     4321 gcgcagatcg ccatctgcgc cgttctggtc acctcatcgc tggtcgcggt tcgtggcctc
     4381 atgcgctccc tgcacagcaa cttcggcttc gatcccaacc atgtcgtcgt ggtcgctacg
     4441 gatttgcaga tggcaggcta taaagaagcc gaccgcgcgc ccatggagcg ccgcatggtc
     4501 gaggcgatcc agtccattcc cggcgtatct gcagccggct attccaatca gttgccgctc
     4561 agcctcggcg gaagcgactc cagcgtctac aaggacacca ccaccgactt ccggcccacc
     4621 aatgccgctg cagacgccat gaactactcc atctctccag gctatctccc cgcggcgggc
     4681 accacactgc tggcgggcag aaacctcact ctgcacgacg acgacaaggc gcccatcgct
     4741 gcgctgatca atcgcgaatt tgccgtgaaa gtgtttggct ccgtcaacgg cgcgctcggc
     4801 ggccacttca aatactgggg cggcaaacga gccgaagtcg tcggtgtggt tgagaacgga
     4861 aaataccgca ccctgaccga agaccagcag ccggccatgt tcttctcctt cctccagcag
     4921 cccgacagcg gcgcctggct ggaagtccgc tccatgcagg accccgcaac cacttcacga
     4981 gccgtggagg agacgctgca ccgcctcgac ccgggactgc ccctccagat gagtacatgg
     5041 aatcaggaga tgagctccgc gctctttgcc gcccgcgtgg ccacagtagc gctcggtgtg
     5101 ctcggcctgc tcggcgcgat gctggccgtc accggcatct tcggcatggc ctcctacgtc
     5161 gtcaccaaac gcatgcgcga gctcgggatc cgcgtcgccc tcggcgctcg gcaaaaacaa
     5221 gtcctgaagg ccgcgctcgg acgagccttt atcctcctcg ccagcggatc ggtcgcggga
     5281 atcgtcctcg gcgtgctcgc taccaaactc ctctcctaca tcgtctacca ggccacgccg
     5341 aaagatccct tcgtcctcgg cggcgtcgtg ctcaccatga tgctgctggg tctgctcgcc
     5401 gcatggctcc cggcccagaa agccctggct gtcgatccca tgatcctgct ccgcgacgag
     5461 taagcagtcg gttcccaatg ggttgttcgt tttcgaacaa cccctcccct ctccggacac
     5521 cgctctgccc gttgacttgc ataaaccctc tcattgcacc gggtgcccct ggcccgccga
     5581 ttgctcaact tcagccggga tcgcccagcc cgacttcagc ctgcacagga gccatcatgc
     5641 tgccagactt gtggcatgcg ttccgtcagt tgcgcaaatc tccaggcttc accctcaccg
     5701 ccgtgcttac cctggccctc ggcgttggcg ctaccactgc catcttcacg ctggtgcagc
     5761 aggtcatgct gcaatcgctc cccgtgacga agcccgaaca actctggcgc atcggcgatc
     5821 agccccgctg ctgcaactgg ggcggataca gtcagagcgg cgccgacgag cccaacgact
     5881 ggagcctctt tccctgggaa gcctaccggc tctttcgcgc caacacacca ggcttccagg
     5941 atctcgccgc actccaggcc ggcaacgccg cgctcggggt acgccctgct ggatcgcagg
     6001 cgccgcccga caccgccaac ggacaatatg tctccggtaa tttcttccgc actctcggcg
     6061 tatccgcatg gcgcggtcga ctcctgaccg atgccgacga tcagcagggc gcaccgccgg
     6121 tgacggtcct cagcttccac gcatgggtgc agaagttcgg ttccagtcca tccgttgttg
     6181 gctccgtgta ccagatcaac ggccatccct tcaccatcgt cggtgtcgcg gcgcccagct
     6241 ttatcggcgc caaaatgacc agctatggca tgcccgacct ctggctgccg ctcaccgccg
     6301 agccgatcat cgacggcgca accacgcgac tcaaaaaccc aaacgtggac tggctcgacg
     6361 tcatcggccg cgtacgccct ggcaccagtc cgtcggctct tcaggctcag ttgcaggccg
     6421 aactgcacgg ctggctcggc agccatctgg ccgacatggg ccccacggaa aagccgctct
     6481 ttgaaaaaca gtctctccgc ctcactccgg gtggtgccgg attttcgagt atgcgcaggg
     6541 attatggcca gggtctgctg ctgctcatga tcaccgccgg ctgcgtactg ctggtcgcct
     6601 gcgccaatat cgccaacctg ctgctcgcgc gtggactgcg caaccgccag caaaccgcgg
     6661 tgcgcgtcgc cctgggcgcg acgcgaggac gcctggtcag gaaggccctc ctcgaaagca
     6721 tctcgttggc attcattggc gccgctgccg gcgttgccgt gtcctgggcg ggggcgcggc
     6781 tcattctcta tctcgccttc cacacccagg ccaaccgcac gtggatcccg gttcacgctt
     6841 caccttccgc ttccgttttg ctctttgcgc tgggactctc cgtcctgacc ggagccctgt
     6901 tcgggatggc gcctgcctgg atgacctcgc gcgccgagcc catcgaggcc ctccgcggtg
     6961 ccagtcgctc cgttggcggc ggccgccact gggcccagaa gacgctggtc atcgctcagg
     7021 cggccgtctc gctggttctc ctgagtgccg cggccatgct cggcggaagc ctgcgcaacc
     7081 tggtgcatca ggacttcggc ttcactccgc agggccgcgt cctggtctcc attaatccca
     7141 gcctctcaaa ctacaaacag gcgcaactgg ttcccctttt ccgtgagatt caggagcgcc
     7201 tcagcgccat ccccggcgtt cgcagtgtga gcgccgcaac ctacgcgccg ctgagcggcg
     7261 atcagtgggg ccacggaatt cgcgtcgaag gccatcccga ccccggacct gaagacaata
     7321 cgatggccga ctggactcgc atcacccctg ggtttttcga caccatcggt acccccatgc
     7381 tcgagggccg ttccatcacg gaagcggaca acaccaccac acgccccgtg gccgtcgtca
     7441 atgaggcctt cgcgaagaaa ttcttcggca accagaaccc gatcggacag cacttcggtc
     7501 cgcaggtgcg aaagaacgcc gccacctacg agatcgttgg cgtcgtccac aacattcatt
     7561 acgtcagcta tgacttccga gcgccggccc gcgccatgta ctacatcgcc gaagcccagc
     7621 ccgtccattt cgacgaaccg gatacccaaa cgtacgaact ctggtcgcag aatctgtaca
     7681 acatcgtcct ctgggctccc ggccatccgt ccagcctcgc ggcccaggtc aggagcgctc
     7741 tggccgacgt cgatccgacg ctggtgatgt acggtgtaga gccctacccc gaggtcatgg
     7801 caggagcctt cgatcagcag aatatgatcg ccagcctcac ctggttgttt ggagcggtcg
     7861 gactgctcct ggccgccatc ggcctctacg gcgtgaccgc ctatggcgtg gaacaacaga
     7921 ccagcgagat cggcgtacgc atggccctgg gtgcggatcg ccgttccgtc atcgccatga
     7981 tcctgcgcgg agctttctgg caggtgggca tcggcctcgg catcggaatt ccggccgcca
     8041 tcggcaccgg ctatctgatc gcaagccagc tcttcggcgt cagcccctgg aatcccctgc
     8101 ttcttgccgg cgccactgca ttgttgggcc tggctgcgct ggtggccgcg ctgattcccg
     8161 cccaaagagc cgcgag
//
LOCUS       DASZYN010000591         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_606974, whole genome shotgun sequence.
ACCESSION   DASZYN010000591 DASZYN010000000
VERSION     DASZYN010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606974"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VHE33_15340"
     CDS             complement(<1..782)
                     /locus_tag="VHE33_15340"
                     /inference="COORDINATES: protein motif:HMM:NF017893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HVT98878.1"
                     /translation="MSDVARLPGEVMAKIQFRSMGVKLIVVCGLALVMVIPALFVWAL
                     VEDRTHRAKDVVNEISQYAGGQQTFLGPTLAIPYTVPAASPSEGTRHGIYLVFPSRAV
                     GAVKVATQERHRSLFKVPVFRADVELSGNFDLSGVPMAAPPGAALQWDQAEIVVGTSD
                     ARGALADAVLTVSGKALTMFPATVAQNLSVGGEGSPLVKLTLLGAKPDEVIEPNASFD
                     VTAKLNFSGAQRVAVLAYGKTTELSMKGDWPNPGFDGGFLPAA"
     gene            complement(838..1224)
                     /locus_tag="VHE33_15345"
     CDS             complement(838..1224)
                     /locus_tag="VHE33_15345"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HVT98879.1"
                     /translation="MKAKATEGRFAYGGLDRVIHERARLSVLTSLVTNPKGLTFGDLK
                     QLCSLTDGNLSRHLRVLERGKMVEIVKGIDRNRPQTVARITASGRKRYLDYLETLEQV
                     VRDAAKGAEEDSSALMRGLRPSRAQG"
     gene            complement(1205..1828)
                     /locus_tag="VHE33_15350"
     CDS             complement(1205..1828)
                     /locus_tag="VHE33_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98880.1"
                     /translation="MNDLHKALGEISSIRRQVARATEFRGYGPATLATTGALAAIAAG
                     LQSWAVPDAAHHLGGYLAIWILTAVVASSLAGVEVYRRTQRVHSRMSDEMIRMAVEQF
                     MPSFAAGLLITIVVLRAVPGVAWMIPGLWQIIFSLGIFASCRFLPRPIVAAGVWYLLT
                     GLILVSFGDARAFSPLAMGIPFSVGQWLVAGILWWSTKKGEHEGESN"
BASE COUNT          457 a          819 c          551 g          414 t
ORIGIN      
        1 gctgccggca gaaatcctcc gtcaaatccc ggattcggcc agtctccctt catgcttagc
       61 tccgtggtct tcccgtacgc cagaactgca accctctgcg cccctgagaa gttcagcttc
      121 gctgtcacgt cgaaactcgc gttcggctcg atgacttcgt ccggcttagc acccagcaac
      181 gtgagcttca ccagcggact tccctcgcct cccaccgaca ggttctgagc gaccgtggcc
      241 gggaacatcg tcagcgcctt cccgctcacg gtcaacacgg catctgccag ggctccgcgc
      301 gcatcactcg ttcccaccac gatctccgcc tgatcccatt gcagtgctgc acccggcggc
      361 gccgccatgg gcacgccgct caaatcgaaa ttcccgctca actccacgtc agcccgaaac
      421 acgggcacct tgaacaacga acgatggcgc tcctgggttg ccaccttcac tgcgccaacc
      481 gctcgtgaag gaaacaccag atagatccca tgtctcgtgc cttccgaggg cgaagccgcc
      541 ggaaccgtgt aaggaatagc cagcgtcggg cccagaaaag tctgttgccc gcctgcgtac
      601 tggctgatct cgttcacaac atccttcgcg cgatgcgttc tgtcctccac cagggcccag
      661 acaaagagcg ccggaatcac catcaccagc gccagcccgc acaccacgat cagctttact
      721 cccatcgacc ggaactggat cttcgccatc acctcgcccg gcaaccgcgc cacgtcactc
      781 atatcgatct cctcatcctc gccaacttta cactgcaaag ttacccgtca aaaaaaatca
      841 gccctgcgcc cgcgacggcc tcagtccgcg catcagcgcg gacgaatctt cctcggcacc
      901 cttcgccgcg tcccttacca cctgctccag cgtctccagg tagtcgagat accgcttcct
      961 ccccgacgcc gtaatgcgcg ccaccgtctg cggacggttg cgatcgatgc ccttcacgat
     1021 ctccaccatc tttccgcgtt ccagcacccg caggtgccgg ctcagattcc catccgtgag
     1081 cgaacacagc tgcttcagat ccccaaacgt cagccctttc ggattcgtca ccagcgaggt
     1141 gagcacactc aaccgcgccc gctcgtgaat cacgcggtca aggcccccgt aggcaaagcg
     1201 cccttcagtt gctttcgcct tcatgctcgc ctttctttgt ggaccaccac agaatccccg
     1261 ctaccagcca ttggccaaca ctgaatggaa tccccatcgc caacggcgaa aatgcccgcg
     1321 catccccgaa cgaaaccagg atcagtccgg tcagcaaata ccaaaccccc gccgccacga
     1381 tcggccgcgg caaaaaacgg cacgacgcga agattcccag gctgaagatg atctgccaca
     1441 atcccggaat catccatgcc accccgggca ccgcgcgcag caccacaatc gtgatcagca
     1501 gccccgccgc aaaggacggc atgaactgct ctaccgccat ccggatcatt tcgtccgaca
     1561 ttctggaatg cactcgctgt gtccgccggt acacttccac accagccaga gacgaggcca
     1621 ccaccgcggt caaaatccag atcgccagat atccaccgag gtgatgcgcc gcatccggca
     1681 ccgcccagct ttgcagccct gccgcgattg cggcgagggc accggtcgtt gccagcgtcg
     1741 ccggaccgta cccgcggaac tcggtcgcgc gcgccacttg ccggcgtatg ctgctgatct
     1801 cacccagcgc tttatgcaga tcgttcatcg gcaattaact ttgcactgca aagctaattg
     1861 caggcgtggc ccttgtcaat ctctaatttg ctgtcgaggt tcagggtcgc gaggcgcgaa
     1921 gcgcgtctgc ctgaacggca agtacaaaaa aaggagggcg accgcttgcg cgatacgccc
     1981 tctggcaacg attcatgagg attcgtgagt tgaaaccacc tcgtcccgcg atccttcagc
     2041 cctgggggag ggactttctc caacctggtc gaactaccgg cccatcacca cgcgactcgg
     2101 agcttcctgg ttgtgcttct tgccgtccag acgcgctgtg atcagactgc gatactgcac
     2161 cgactgcagg gtgtagttgt caccctcttt gtcgaaccgc agaaccacgc gagcatcggt
     2221 cggcgccggt tcgccaggac c
//
LOCUS       DASZYN010000592         1700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_34911, whole genome shotgun sequence.
ACCESSION   DASZYN010000592 DASZYN010000000
VERSION     DASZYN010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34911"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            670..>1700
                     /locus_tag="VHE33_15355"
     CDS             670..>1700
                     /locus_tag="VHE33_15355"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HVT98881.1"
                     /translation="MAGAGESAPFEARYNPAEIEAKWQARWEAQPGLYAAEPASSAKS
                     KYYVLEMLPYPSGQLHMGHVRNYAIGDALARYMWMQGHNVLHPMGWDAFGLPAENAAI
                     RNNTPPRQWTLQNIAAMKRQMNRLGLSYDWSNEVTTCLPDYYRWNQWFFLRMYERGLA
                     YRKKSKVNWCPQCATVLANEQVVDGCCWRHEDQIVEQRDLEQWFLRITRYAQELLDDL
                     SKLEGWPEKVRTMQRNWIGRSEGAQVEFELEDAVSAPEDDDKARKSGTAILPAREGVK
                     GSGKKIAVFTTRIDTIFGATSLQLAPEHPLVKEMAADDARLSDEVAALLEQQRKARET
                     GDVGAIEKHG"
BASE COUNT          379 a          590 c          453 g          278 t
ORIGIN      
        1 acgcaaagcc tggacaatgc cgtatccatg ctgcggtccc cattgcaggg tctggaggat
       61 gagcatgtcg agtgttccct gcagcagctc gatccggttc ttgtactggt ctttttcgct
      121 catggatgta gacactctac atccaacgag gtagatcgtc tactagtggc ccatcgcaga
      181 gagttcgtta ccggttttga aagggaaggg cttttagtcg tgccgaaact cgcaaggtac
      241 agatcgggca gagaggtcac ggtacagacc ggggacatgg gtaatacttc gcgccgggag
      301 cggggcgata tgccctcgct ccagacacat agcggttgac aaacttgtaa actagcagaa
      361 actccttccg aatcaatcat ctgcagtcga agccccttag aggaaatcca tccccattgc
      421 ccgcacccat ccgaatccag tcccggctcc actcgacttc cgtcatcact ccgccaacga
      481 gtaggggccc cgccaaaagc tctcaaatcg aatcaacaac ttcccagggg gtcgcccccc
      541 tttgcgccga aattcagagg gatcgtccgc cacatccgcc agccccgggc agtcccccct
      601 ggctgtgcaa tttacaatca gacctggacc cgtcctatgg cgacaaaaga acaagcagaa
      661 gctgcccgag tggcaggcgc cggagaatcc gcccctttcg aggctcgcta caaccccgcc
      721 gaaatcgagg ccaaatggca ggctcgctgg gaagcccagc ccggcctcta cgccgccgag
      781 cccgcctcct ccgccaaaag caagtactac gtcctcgaga tgctgcccta tccctccggc
      841 cagctccata tgggccacgt ccgcaactac gccatcggtg acgcgctcgc ccgctatatg
      901 tggatgcagg gccacaacgt cctccacccc atgggctggg acgccttcgg cctccccgcc
      961 gagaacgccg ccatccgcaa caacaccccg ccgcgccagt ggaccctcca gaacatcgcc
     1021 gccatgaagc ggcaaatgaa tcgcctcggc ctcagctacg actggtcgaa cgaagtcacc
     1081 acctgcctcc ccgactacta ccgctggaac cagtggttct tcctgcgcat gtacgagcgc
     1141 ggcctcgcct accgcaaaaa gagcaaggtc aactggtgcc cgcaatgcgc caccgtcctc
     1201 gccaatgagc aggtcgtcga cggctgctgc tggcgtcacg aggatcaaat cgtcgagcag
     1261 cgcgacctcg agcagtggtt cctgcgtatc acccgctacg cccaggagct actcgacgac
     1321 ctcagcaagc tcgaaggctg gcccgaaaaa gttcgcacca tgcagcgcaa ctggatcggc
     1381 cgcagcgaag gcgcccaggt cgagttcgaa ctcgaggatg cggtgagcgc tcccgaggac
     1441 gacgacaagg cccgcaagtc cggcaccgcc atcctccccg cacgcgaagg cgtaaagggt
     1501 tcaggaaaaa agatcgccgt cttcaccacc cgcatcgata ccatcttcgg cgccaccagc
     1561 ctgcagctcg cgcccgagca tccgctcgtt aaagaaatgg ctgcagacga cgcccgcctc
     1621 tccgacgaag tcgcggccct cctcgagcag caacgcaagg cccgcgaaac cggcgacgtc
     1681 ggcgcgatcg aaaaacatgg
//
LOCUS       DASZYN010000593         2993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_480104, whole genome shotgun sequence.
ACCESSION   DASZYN010000593 DASZYN010000000
VERSION     DASZYN010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480104"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1588
                     /locus_tag="VHE33_15360"
     CDS             <1..1588
                     /locus_tag="VHE33_15360"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HVT98882.1"
                     /translation="TPEASTTTFDVLNSASTSSASVITAGQSVSYGTQLILAAQVHGT
                     SQAPTAPTGTISFTDGGNPLNTAPINAEGDAEFNAPFAVGSHTVNASFSGDGSYLASS
                     GTALAFTVGKNTPNLELATSVQTSAGSLPSGQTDVVTVVLLNSANLAAGSGTPTGLPY
                     TVSAAAPTGSLSISGISGVSSATLAAGVDPGTGEPAGIATFTVPASSATGSPTMTVTY
                     NGDANYNSVSGHTSIPFGSTGGTATTTTASIAGSISPTTSVTLSGTVSGNGTAAPTGS
                     IEIYAGGYVLGYASLSPKTAQIKTDPQTQPFRLLLGGSGVAFACVLLLTIPARRSAWR
                     NMLGLVLCVCLVTFGIGCGGGGGSGGGGGGGGGGGGGGGGTPQTSTFTVQLTSASLLQ
                     GSNTITVQYLPATGSPYAPSSFTLSTPLDNPLSDFSLTASTSIVSVTAGTGTAKLFVA
                     STNGFSGTVNLGCSVVGSPTGVSCALSQPSAALTAGSSVAVTVTVSTTGSPAAGNYPI
                     SITGTAGSQIHTLGVTAAVH"
     gene            1608..1745
                     /locus_tag="VHE33_15365"
     CDS             1608..1745
                     /locus_tag="VHE33_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98883.1"
                     /translation="MIPLLLRGAGFAVWAAVLALQTDPEADQETEIRLPAATLRVRIL
                     L"
     gene            1824..>2993
                     /locus_tag="VHE33_15370"
     CDS             1824..>2993
                     /locus_tag="VHE33_15370"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HVT98884.1"
                     /translation="MNGTRARGSEGAAAGRAAAGLERPGPVLAPRAPRLAERPTVIRQ
                     PRRWFYALWIAGLVVLFCLHFPHLLADFPNDSPWMDYAKYTDEGWYGNAAVRHALTGH
                     WSLQGDFNPAVALPVWPLLLAGVFHFTGVSLGADRSLALIFFGLDLLLAYSVVRSQAE
                     RWAALLAVTLLATSPFLWAFSRLAILESPLVFFLLLSWLLALRLPRTAPRWRTAMLAG
                     IGLLLCLMVLTKTTAVFLIPSSLFLVARAWTRRREAVRALGICGAAAALPWCAWYFLA
                     VRPHYRVDYRYFFEANRWPQTATLGGWAGSFWWALHGCFWISPAICIVAIAVLLLALF
                     RRNGPALVVIGSRFPAGLYASPLTPASLLAIAGYVAFIGWHNNPQPRYYEVVIYPV"
BASE COUNT          457 a         1002 c          947 g          587 t
ORIGIN      
        1 aacgcccgag gccagcacca ccacctttga cgtgctcaac tcggcgtcta catccagcgc
       61 cagtgtgatc accgcgggtc aaagcgtctc ttacggcacg cagctgattc ttgccgcgca
      121 ggtccatggc accagccagg cgccgaccgc gcccaccgga acgatcagct tcacggatgg
      181 cggaaatccg ctgaatacgg cgccgatcaa cgccgaaggc gatgcggagt tcaacgcgcc
      241 gtttgctgtc gggagccata ccgtcaatgc ttccttcagc ggcgatggca gttacctggc
      301 atcatcggga accgcgcttg ctttcacggt tgggaagaat actccgaacc tggaactcgc
      361 caccagcgtt cagacgtcgg cgggctctct gccctcgggt cagaccgatg tcgtcaccgt
      421 cgttctgctg aacagcgcca atcttgctgc cggcagcggc acgccgaccg gccttcccta
      481 cacggtctcg gcggctgccc ccacgggcag cctgagcatt tccggaatct ccggcgtcag
      541 cagcgcgacg ctggccgcgg gggtcgatcc agggacgggc gagccggcgg gcatcgccac
      601 tttcaccgtg ccagccagtt cagcgacggg cagccccacc atgaccgtca cctacaacgg
      661 cgatgcgaac tataactccg tgagcggaca cacctcgatc ccgttcggct ccaccggtgg
      721 caccgcgacc acgaccacag cctcgatcgc gggcagcatc tcgccaacga cctcggtcac
      781 gctgagcggc acggtaagcg gcaacggcac cgctgcgccg acgggctcga tcgagattta
      841 cgccggagga tacgttctcg gatacgcctc gctaagcccg aagaccgcgc aaatcaaaac
      901 cgatccgcaa acgcagccct tccgattgct gctcggcggc agcggtgtgg cttttgcgtg
      961 cgtcctgctg ctcacgattc cggcgcgccg cagcgcatgg cggaacatgc tgggcctggt
     1021 cctgtgcgtc tgccttgtga ccttcgggat tgggtgcgga ggcggaggcg gatcgggcgg
     1081 tggtggtggt ggcggtggcg gcggcggtgg aggaggtggc ggcactccgc agacttcgac
     1141 ctttaccgtg caattgacca gcgcgtcgct gctgcagggc tccaacacga tcacggtgca
     1201 gtacctgccg gcgaccggca gtccctacgc tccgtcgtcg tttacgttga gtacgccgct
     1261 ggacaacccg ctgtccgact tctctctcac cgcctcgacg tcgatcgttt ccgtcacggc
     1321 cggaaccggc acggcgaagc tcttcgtggc gtcgacgaat ggattcagtg ggacggtaaa
     1381 tcttggctgc agcgtggtgg gttcgccgac gggcgtttcc tgcgcgctca gccaaccatc
     1441 ggctgcgctc acggcgggct catctgtagc agtcacggtt acagtcagca ccaccggatc
     1501 gccggctgcg ggcaactacc cgatcagcat tacgggcacg gcagggagcc aaatccacac
     1561 actgggagtt acagcagccg tccactaagc aacagaaacg ccggcgcatg atcccgctcc
     1621 tcctccgggg agcgggattc gccgtttggg ccgcggttct ggcgctgcaa acggatccgg
     1681 aagcggatca ggagaccgaa attcggctgc cggctgcaac cctccgcgtc cgtatactgc
     1741 tctgaaatcc cagccggcgc cattcagggc gctgccgggt gcgctttcag gatttcactc
     1801 ctgtcagaag agacggccag atcatgaacg gaacgcgagc acgcgggagt gagggagcag
     1861 cggcgggccg cgcagcagcc ggactcgagc ggcccggtcc tgtgctcgcg cctcgcgccc
     1921 ctcgcctcgc ggaacggccc actgtcatcc gccagccgcg tcggtggttc tacgcgctgt
     1981 ggatcgccgg gttagtcgtg ctcttctgcc tccatttccc gcatctgctg gccgacttcc
     2041 ccaacgactc gccatggatg gactacgcca agtacacgga tgaaggctgg tatgggaatg
     2101 ccgccgttcg ccatgcgctg accggacact ggtctctgca gggtgatttc aatccggccg
     2161 tggcgctgcc ggtttggccc ttgctgttgg ccggggtctt ccacttcacc ggagtgagcc
     2221 tcggcgcgga tcgcagcctg gctctgattt ttttcgggct cgatttgctg cttgcgtata
     2281 gcgtggttcg cagccaggcg gagcgatggg cggcgttgct ggctgtcacc ctgctcgcga
     2341 ccagcccgtt cttgtgggcc ttcagccggc tggccattct tgaatcgccg ctcgttttct
     2401 tcctgttgct gtcgtggctg ctggcgctcc ggctgccgcg caccgcgcca cgatggcgga
     2461 cggcgatgct ggccggcatc ggcttgttgc tgtgcctgat ggttttgacc aagaccacgg
     2521 ccgtcttcct gattccgtct tccctgtttc ttgttgcgcg cgcctggacg cgccgtcgcg
     2581 aagccgttcg ggcgctgggc atctgcggtg ccgctgctgc gctgccgtgg tgcgcctggt
     2641 atttcctggc tgtgcgtccg cactatcgcg tcgactaccg gtactttttc gaagcgaatc
     2701 gctggccgca gacggccacc ctgggtggct gggctggatc tttctggtgg gcgctgcatg
     2761 ggtgtttctg gatctcgccc gcgatctgca tcgtcgcgat cgccgtgctg ctgctggcgc
     2821 tcttccggcg aaacggccct gcgctcgtcg tgatcggatc ccgcttccct gcggggctgt
     2881 acgcaagccc gctcacgccc gcgagcctgc tggccatcgc cggatacgtt gccttcatcg
     2941 gttggcacaa caacccgcag ccacgctact acgaggtcgt gatctacccg gtt
//
LOCUS       DASZYN010000594         9171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1126199, whole genome shotgun sequence.
ACCESSION   DASZYN010000594 DASZYN010000000
VERSION     DASZYN010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9171
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126199"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..962)
                     /locus_tag="VHE33_15375"
     CDS             complement(147..962)
                     /locus_tag="VHE33_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98885.1"
                     /translation="MTAAYVTLILDVVTSSGAELAQGSVSIAPSMVLPSPADQILVSQ
                     MPYTIYFKGNSYPQVQLVGNDTIGPQQGNGTPGWNYVFTPDAPSGLTAASYYVLYSNG
                     PTQRLSALAASPAAQPGSQTVPLPAAGPTSLTQVLGLADVSPVETEWVPGAAQILNGD
                     GVRPFVVNLEDAPVIAVDASAGNDFRVQLGGNRTIGAPSNPADGQRITISVQQPGSGG
                     PYTPVFSGSAGGFSYGSAFAPSWSTAANAIDITGWLYRADKGVWCYTGAGLGF"
     gene            complement(959..3175)
                     /locus_tag="VHE33_15380"
     CDS             complement(959..3175)
                     /locus_tag="VHE33_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98886.1"
                     /translation="MALTVTATASGTGSVNGTSLVVRVLNNASLTQNGATANSSTVTT
                     PQISITPHATGSMIYGSIVTDITSLSSLDANTTALVNQAASISGNGVATYAGYRSTSL
                     TASTSAATYGWGAPTVGAGEIFIASAEILASGGTLNEDASSPAAAWNPSSLSATTASF
                     TPPASAVLVAIVGAGWNTSGSTAVTVTDSASAYNWSQLSGVPTTDSAAIWVGIPKNTS
                     VTVSAGLATATAAARTPAVTNSGTAAAGLAAATAQAAVSPVNSFLRDTSGAQITDTAG
                     SPVLDTSAPLTLNVLIQPGAGRAQGTAQALAASASSSSAVTAGRAQATAAALAPSVTV
                     SASVTAGLATAAATAQAPAAQVTPPAGRGQSSAAAQAPAAAVTATAGLAQATAAALAP
                     SVTASGNAVAGLATAAAAAQAPTVTESGSANAGLATATGAAQAPAAALTAAAGIASGS
                     TAAKSPTAQVTAQAGLAHAAAQGLAPAVSAAFSAGRAQATGTAQAPAALVQPGAGRAQ
                     ATGTAYNAPVSSDITVTAGLATATATAQSVTTHLVRPPLHLGASVSYLQYGGEVSEET
                     LGGSAATVANLLGGSAVPVNAYGGSLTTGNSLLGGTSTPVSNTFGGTLNYNAFGGSLT
                     GWTMQQTTLAISEFNDETINIAITNNGSPYNLTGVTVQMLFKTAAGTPDNEALVFSSS
                     GGSPAITITNAAGGLATAVIPNTDLDAETYNFFRIDVVVSGLINTCLYGPITWTSL"
     gene            complement(3206..3676)
                     /locus_tag="VHE33_15385"
     CDS             complement(3206..3676)
                     /locus_tag="VHE33_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98887.1"
                     /translation="MAWSTSNLSGAGVSALIGGSFYGTSQTFASDTINAALYGNTGTP
                     NKDDTLAHNAYNGTGGQWVTANEVTGTNYTAGGTALTSKSNTEATGTVTVTAANPSWA
                     SATISNVYGTLVYDNTVTNKYAYCWNYFGGSQSVTSGTFTVQWNASGIFTVTIS"
     gene            complement(3752..4075)
                     /locus_tag="VHE33_15390"
     CDS             complement(3752..4075)
                     /locus_tag="VHE33_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98888.1"
                     /translation="MNIPLLNIQPQRWYCPNCACTDITPPVPPGQSQYHACPGLHGLN
                     APLIREGVRAKVEAAEREDYLNGDQQATGDNGKVYMNVRVTRDEGEDVTVFAGLAHAD
                     LRNMQ"
     gene            complement(4163..6193)
                     /locus_tag="VHE33_15395"
     CDS             complement(4163..6193)
                     /locus_tag="VHE33_15395"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HVT98889.1"
                     /translation="MGEWDLYGTLTTPASNDTLLINDVSATPPAAGTVKQITYAALAG
                     LQPSGVTSGSTDTANIQALLTAGGRVVLGPGTFYISNMLQIPSGTCLSGAGKGVTTIK
                     AASGSWSSVAQVNLINGLGLLITSGNTTASYIAVRNLTVDMNQTGITALPSWATGGTA
                     AGDNVTNAGIHLNNVTNLLVEDVEVINTIGYTVYLQSCSDFTVRGCRVISGQVTATQS
                     WGLPSQQDGIHLDSCTYGQVDGNYIDTGATGVNTGDDCVALQSFAAVHDISITNNTMR
                     SDEAAVDFALTGADIYNISVTGNNIWQTEESAITVQPFAGSGALARNITISGNTISNF
                     AVNGNSPRAAVALYDYSLFTTAATAWTGISITGNTIQTTTNTSAFGVYARYGTSLTVS
                     GNTIRDFPGTLGIILGDNEAGTSLPVRQFSVTGNTVDMSTSAVTGCVGILPYDASSGV
                     VSGNFVTGPGGNASDRIGISPTQASGTNLVQNISVSGNTLTGWDFGIQETNAGGGNPD
                     SNAYTGNVFSSCTQNYQLLGTHNLVSASQRTTLQSAFTMASSASAQNVTGLAVTLQPG
                     TWRVTGWWPMQQSAGTGTLQFAFTFGGTASAANCRYILTGATSSTTALETSLTALSSA
                     SPALSASGMWGDFTGLVTVTAAGTLQLQVKASATSVQAVFPVGAYLEVEPLA"
     gene            complement(6256..6462)
                     /locus_tag="VHE33_15400"
     CDS             complement(6256..6462)
                     /locus_tag="VHE33_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98890.1"
                     /translation="MYPYPYTFDVERIPLTRDEQLAFTKIVVAECSVQDQHGFSSFLA
                     ALRGPCGPTLCDTAASLVTRIWRR"
     gene            complement(6417..6740)
                     /locus_tag="VHE33_15405"
     CDS             complement(6417..6740)
                     /locus_tag="VHE33_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98891.1"
                     /translation="MSLLQFVRHFHITGAGWYWIIWFFGGFGVYEGWALIYNTQDTLS
                     WQFWGLEQVNFSDPVQFSTWTPVHYAIGVILLAGMVWLFGHLVFGIWRLCTPTRTRST
                     WSASR"
     gene            complement(6737..6985)
                     /locus_tag="VHE33_15410"
     CDS             complement(6737..6985)
                     /locus_tag="VHE33_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98892.1"
                     /translation="MDNLWNLLPPWLATRAKAFLAAAGGIAYVAVSFFPSVASNHWVA
                     AVIGILTFLGVYTAPNSNARSQARQSGPRNPDRTVTTR"
     gene            complement(7020..7598)
                     /locus_tag="VHE33_15415"
     CDS             complement(7020..7598)
                     /locus_tag="VHE33_15415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HVT98893.1"
                     /translation="MTKAPAFTPIADLQFTNLSLVIRQVQETEHRWFAQGDPKSPTLP
                     WMPFQQSAFLAMLYDCIPEMSGRKFLDVGCGPGTKMQLAKHVYGLDVSGLEISPEMAR
                     AAGAVGTVTCVDALKAPRGTFSSYDLIWLYRPFRDSELEWLLESRIMKEMKPGAILAG
                     GAWEICPADHKWVTVVDDWELRHGAWMKPQTA"
     gene            complement(7595..7756)
                     /locus_tag="VHE33_15420"
     CDS             complement(7595..7756)
                     /locus_tag="VHE33_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98894.1"
                     /translation="MTNPEPVDDREPAPAEMRGGDRPEDYVQLLPPTERELMEAEVEA
                     FMTRKEDGE"
     gene            complement(7771..8403)
                     /locus_tag="VHE33_15425"
     CDS             complement(7771..8403)
                     /locus_tag="VHE33_15425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HVT98895.1"
                     /translation="MVIPRVSIITPTWGRHERLLNRCIPSVQAQTWRNTEHVIVSDGP
                     DPELLEKIGKLHESGAFYHPVRFVQLAEHSPEQHWGHLARLAGIEASGGAYIGYIDDD
                     DLLMPEHCAKLAAALTQNPGAGWAYSVMRSWSSSGLCTEIGHGQLACGNLGTPMIMHR
                     RETLEHGTWGPASSVEDWELVSRWTAAGVQYVHVPEATAEVWPSVYHVHY"
     gene            complement(8397..9131)
                     /locus_tag="VHE33_15430"
     CDS             complement(8397..9131)
                     /locus_tag="VHE33_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98896.1"
                     /translation="MRDLLMVIPSRGRPGNVARLSDAADATCEGDTWFEIGLDNDDPT
                     AEHYPVGKRLTYTMEADLHKVTAWCNHLAVPEATAFRYIGHFGDDNVPETPGWDVKIM
                     EALEQTPFAFANDLYPGREPGTLCCHVFMRSEVVRTLGYFGPPSIAHMYVDVAWMAWG
                     RACGITYLHDVLIPHLHYTTGRSGNDATYQNSYAGTGADLQAWHAYCRSGQLNEDIGK
                     LGGRPYSDQELAEFNRGLNIPEVWPW"
BASE COUNT         1462 a         2877 c         3216 g         1616 t
ORIGIN      
        1 tggtcaggga gctggtggtg aaaccgacga tcaggacccg gttcgcgccg ctgccgcagg
       61 tatggctcca gctcagggta ctgacgcttg agccggtcgc cccgctggac gacggcccga
      121 ccgcgtcgaa cgcgatggcc atcgggtcag aaccccagtc cggctccggt gtaacaccac
      181 acgcccttgt ctgcccggta cagccagccg gtgatgtcaa tggcgttcgc agcagtcgac
      241 cacgacgggg cgaaggctga cccgtaactg aagcccccgg cggaaccgga gaacaccggg
      301 gtgtacggtc ccccggatcc tggctgctgg acggaaatcg tgatccgctg gccgtcagcc
      361 gggttggacg gtgcgccgat ggtgcggttg ccgccgagct ggacgcggaa gtcgttgccc
      421 gcgctggcgt ccacggcaat gacaggggcg tcttccaggt tgacgacgaa cggcctgacc
      481 ccgtccccgt tgaggatctg cgccgcgccg ggaacccact cagtctccac cgggctgaca
      541 tcggccaggc cgagcacctg cgtcagcgag gtggggccgg cagccggcag cgggaccgtc
      601 tgcgaaccgg gctgcgccgc aggtgacgcg gccagtgcag acagccgctg agtgggcccg
      661 ttgctgtaga gcacgtagta gctggcggct gtcaggcccg agggagcatc cggggtgaag
      721 acgtagttcc agccgggggt gccgttgccc tgctgcgggc cgatggtgtc attgccgacc
      781 agctggacct gcgggtagct gttgcccttg aagtaaatcg tgtagggcat ctgggagacc
      841 aggatctggt cagccgggga cggcagcacc attgacggcg cgatagacac cgagccctgg
      901 gccagttcag cgccgctgga ggtcaccacg tcgaggatca gggtgacata ggcggccgtc
      961 acagcgacgt ccaggtgatc ggcccgtaca ggcacgtgtt gatcaggccc gacaccacca
     1021 cgtcgatccg gaagaagttg taagtttccg cgtcgaggtc ggtgttcggg atgaccgctg
     1081 tggccaggcc gcccgccgcg ttggtgatgg tgatggccgg gctgccgccg ctggagctga
     1141 acaccagcgc ctcgttgtcc ggggtgcctg cggcagtctt gaacagcatc tgcaccgtga
     1201 cgccggtcag gttgtagggg ctgccgttgt tcgtgatggc gatgttgatg gtctcatcgt
     1261 tgaattccga gatcgccagc gtagtctgct gcatcgtcca tcccgtcagg cttccgccga
     1321 aggcgttgta gttcagggta ccgccgaagg tgttgctgac gggagtgctt gttcccccga
     1381 gcaggctgtt tcctgtggtc agcgagccgc cgtaagcgtt caccggcacc gcagaaccgc
     1441 ccagcaggtt cgccacggtg gcagccgagc cgcccagggt ctcctcggaa acctcaccgc
     1501 cgtactggag gtaggagacc gacgctccca ggtgcagcgg cgggcgcacc aggtgggtcg
     1561 tgacgctctg agcggtggcc gttgccgtgg cgagcccggc ggtaacggtg atgtcgctgc
     1621 tgaccggggc gttgtacgcc gtcccggttg cctgtgcccg gccagctccc ggctgaacca
     1681 gggcagcagg agcctgggct gtccccgttg cctgcgcccg cccggcgctg aaagccgccg
     1741 acaccgccgg ggcaagcccc tgggcagccg cgtgcgcaag tcccgcctgg gccgtcacct
     1801 gcgccgtggg tgacttggcg gcagtactgc cgctggcaat cccggctgca gcagtgaggg
     1861 cagctgccgg ggcctgcgct gctcccgtgg ccgtcgcaag tcccgcattc gcgctgcccg
     1921 actcggttac cgtgggagcc tgggcggctg ccgctgccgt ggcgaggcca gcgacggcgt
     1981 ttcctgaagc ggtgacactg ggcgcgagag cggctgccgt ggcctgtgca agccccgccg
     2041 tggcggtgac ggctgccgca ggagcctgcg ctgctgccga cgactgcccg cgcccggccg
     2101 gcggcgttac ctgggcagcc ggggcctgag cggtcgccgc tgccgtggca aggccggccg
     2161 tgacactggc ggaaactgta accgaggggg caagagcagc ggctgtagcc tgtgcccgtc
     2221 cggccgtgac agcactggag gaagaggcac tggcggcaag tgcctgagct gtgccctggg
     2281 cgcgcccggc ccccggctgg atcaggacgt tcagcgtcag cggggcgctg gtgtcgagaa
     2341 cggggctgcc cgctgtgtcg gttatctggg cgcctgacgt gtcccgcagg aaagagttaa
     2401 ccgggctgac cgccgcctgg gcggtagcgg ctgccaggcc agctgctgcg gtgcccgagt
     2461 tggtgacggc cggcgtccgg gcggcagcag tggccgtggc caggcccgca ctgaccgtga
     2521 cagacgtgtt cttggggatg cccacccaga tcgcggcgga gtccgtagtg ggaacgccag
     2581 acagctgcga ccagttgtag gcactggcgg agtcagtgac ggtgacggca gtggaccccg
     2641 aggtgttcca gccggccccg acaatcgcaa ccagcacagc cgacgccggc ggggtgaagg
     2701 aagcggtggt ggccgacagg ctggacgggt tccaggcagc agccggtgac gaagcgtcct
     2761 cgttaagggt tccgccgctc gccaggatct cggcactggc gatgaaaatc tcgccggccc
     2821 ccaccgtggg agcgccccag ccgtacgtag cagccgaggt ggaagccgtc agcgacgttg
     2881 acctgtaccc ggcgtaggtg gcgacgccgt tgcccgatat tgacgccgcc tggttgacga
     2941 gagcggtggt attggcgtcg agtgagctga gggaagtgat gtcggtgacg atcgagccgt
     3001 agatcatgga ccccgtggcg tgcggggtaa tggagatctg cggcgtcgtg accgtagagg
     3061 agttagccgt tgcgccgttc tgcgtcagcg aggcgttgtt cagtaccctg acgaccaggg
     3121 acgtgccgtt gaccgacccg gttccggaag ccgtcgccgt gacggtcagt gccacggggt
     3181 caccgccttc cgggcagtgt gccccttaag agatggtgac ggtgaagatt cccgaggcgt
     3241 tccactggac ggtgaaggtg cccgaggtca ccgactgcga gccgccgaag tagttccagc
     3301 agtaggcgta cttgttagtc accgtgttgt cgtagaccag ggtcccgtag acgttgctta
     3361 tcgttgctga ggcccagctg gggttggccg ccgtaaccgt gacagtgccg gtggcctcag
     3421 tgttcgactt ggaagtcagg gccgtgccgc cagcggtgta gttggtgccc gtaacttcat
     3481 tcgccgtcac ccactggccg ccggtgccgt tgtaggcgtt gtgggcgaga gtgtcgtcct
     3541 tgttcggcgt gccggtgttg ccgtagagcg cggcattgat ggtgtcactg gcgaacgtct
     3601 ggctggttcc gtagaacgag ccgccgatca gggcgctgac gcccgcgccg gaaaggtttg
     3661 aggtagacca ggccacttca gaccgccctt actgtgatga gtgtcggagt tccctcgtag
     3721 accaggtccg cacgagtcgc ggcggggaag gtcattgcat gttccttaga tcggcgtgcg
     3781 ccaggccggc gaacacagtg acgtcttcgc cctcgtcgcg ggtgacgcgg acgttcatgt
     3841 agaccttgcc gttgtcgcct gtggcctgct ggtcgccgtt caggtagtcc tcacgctcgg
     3901 cggcctcaac cttggcccgc acaccctcgc ggatcagggg cgcgttcagc ccgtgcaaac
     3961 cagggcaggc gtggtactgc gactgaccag gcggcaccgg cggcgtgatg tcggtgcagg
     4021 cgcagttagg gcagtaccag cgctgcggct ggatgttcag cagcgggatg ttcacagggc
     4081 tcctggcagg ttagagcccc ggctccgcga acggccgcga ggtacgtctg cctgtatcgt
     4141 agcggccccg gggcaggtgg ggctaggcaa gcggctctac ctccaggtac gcgccgactg
     4201 ggaagaccgc ctggacactc gtggcactgg ccttcacctg aagctgcagg gtcccggcag
     4261 cggtaacggt gacgagcccg gtgaagtcac cccacatgcc cgacgctgac agggcagggc
     4321 tggcactgga caaggcagtc aggctggttt ccagggccgt agtcgagctg gtggccccgg
     4381 tgagtatgta ccggcagtta gcagcgctgg cagtgccgcc gaaggtgaac gcgaactgca
     4441 gggtgccggt gccggcagac tgctgcatcg gccaccatcc ggtgacccgc caggtgccgg
     4501 gctgaagggt aaccgccagt ccggtgacgt tctgggcgct ggcgctgctg gccatcgtga
     4561 acgcactctg cagtgtggtc cgctgactgg ccgagaccag gttgtgagtg cccaggagct
     4621 ggtaattctg ggtgcagctg ctgaaaacgt tcccggtgta agcgttggaa tcggggttcc
     4681 cgcccccggc attggtctcc tggatgccaa aatcccagcc ggtgagggtg tttccggaga
     4741 cactgatgtt ctgcaccagg ttggtgccgc tggcctgtgt cgggcttatc ccgatccggt
     4801 cggaagcatt gccgcccggc ccggtgacga agtttccgga gacaaccccg ctgctcgcgt
     4861 cgtagggcag gatgccgacg cagccggtca ccgccgatgt gctcatgtca acggtgttgc
     4921 cggtgaccga gaactgcctg accggcaggc tggtccccgc ttcgttgtct cccaggatga
     4981 tcccgagggt tcccgggaag tcgcggatcg tgttgccgct gaccgtcagg ctggtcccgt
     5041 aacgggcgta gacgccgaag gcactcgtgt tggtggtggt ctggatcgtg ttgccggtga
     5101 tgctgatgcc ggtccaggca gtggcagccg tcgtgaacag cgaatagtcg taaagggcga
     5161 ccgcagcacg ggggctgttg ccgttgacgg cgaagttgct gatggtgttg ccgctgatcg
     5221 tgatgttgcg ggcgagcgca ccggagccag cgaacggctg aacggtgatc gcagactctt
     5281 cggtctgcca gatgttgttg ccggtaacgc tgatgttgta gatgtcggcg cccgtcaggg
     5341 cgaagtcgac agcagcctcg tccgaccgca tcgtgttgtt ggtgatcgag atgtcgtgga
     5401 cagccgcaaa ggactgcagg gcaacgcagt cgtcgccggt gttgacgccg gtcgcgccgg
     5461 tgtcgatgta gttcccgtcg acctgcccgt aggtgcagga gtcgaggtga atgccgtcct
     5521 gctgcgacgg caggccccag ctctgggtag cggttacctg cccggatatc acccggcagc
     5581 cccggacagt gaagtccgag caagactgca ggtaaacggt gtagccgatc gtgttgatga
     5641 cctcaacgtc ctcgaccagc aggttggtga cgttgttgag gtggattccg gcgttggtga
     5701 cgttgtcccc ggcagcggtc ccgccggttg cccatgacgg cagcgcggtg atgcccgtct
     5761 ggttcatgtc gacggtcagg ttccggaccg caatgtagct ggccgtggtg ttgcccgaag
     5821 tgatcagcag accgaggccg ttgatcaggt tgacctgcgc taccgacgac caggaaccgg
     5881 acgccgcctt gatggtggtg acgcccttgc ccgccccgga caggcaggtg cccgacggga
     5941 tctggagcat gttgctgatg taaaacgtgc cggggccgag cacaacccgg ccgcctgcag
     6001 tcagcagcgc ctggatattg gccgtatcgg tactgcccga ggtgacaccg gacggctgga
     6061 gtcccgcaag cgcggcatag gtgatctgct tgaccgtgcc cgctgccggc ggcgtagcgc
     6121 tgacatcgtt tatcagcagc gtgtcattag acgccggagt tgtcagtgtg ccgtacaggt
     6181 cccactcgcc cacagagcct ccgcctgaga ggccccggct ccgcgtacgg ccgcgaggta
     6241 cgtctgcctg ttatgttagc ggcgccagat gcgcgttacc agcgacgctg cggtgtcgca
     6301 gagggtggga ccgcacgggc cccgcagggc cgcaaggaac gagctgaacc cgtgctggtc
     6361 ctggacgctg cactccgcga cgacgatctt ggtgaaggcc agctgctcgt cccgggtcag
     6421 cgggatgcgc tccacgtcga acgtgtacgg gtaggggtac ataaccgcca gatcccgaac
     6481 accaggtggc cgaacagcca gaccatgcca gccaggagga tgacgccgat cgcgtagtgc
     6541 accggggtcc aggtggagaa ctgaaccggg tcggagaagt taacctgctc caggccccag
     6601 aactgccagc tcagggtgtc ctgggtgttg tagatcagag cccagccctc gtagaccccg
     6661 aagcccccga agaaccagat gatccagtac cagcccgccc cggtgatgtg aaaatggcgg
     6721 acaaactgaa ggaggctcac cgggtcgtca ctgtgcggtc cgggttacgc gggccggact
     6781 gccgcgcctg gctcctggca ttcgagttcg gggcggtgta gaccccgagg aaggtcagga
     6841 ttccgatgac ggcagcgacc cagtgattgc tggcgacaga cgggaagaag ctgactgcga
     6901 cgtaggcaat accgccggcg gcggcgagaa aagctttagc gcgcgttgcc agccagggcg
     6961 gcagcaagtt ccacaggttg tccatacagt gagcataggc gtcttactgc cacgtggcat
     7021 taagcagttt gcggcttcat ccaggcgccg tggcggagtt cccagtcgtc tacaaccgtg
     7081 acccatttgt gatccgccgg gcagatctcc catgccccgc cggccaggat cgcccccggc
     7141 ttcatttcct tcatgatccg gctttccagg agccattcca gctcgctgtc gcggaacggc
     7201 cggtacagcc agatcaggtc gtagctgctg aatgtgcccc ggggagcctt gagtgcgtca
     7261 acgcacgtga ctgtgcccac cgccccggcc gcccgcgcca tctccgggct gatctccagg
     7321 ccggaaacgt caaggccgta gacatgcttg gcaagctgca tcttggtgcc gggaccgcag
     7381 ccgacgtcca ggaacttgcg gccggacatc tccgggatgc agtcgtagag catggcaagg
     7441 aacgcggact gctggaacgg catccacggc agggtcgggg acttcgggtc gccctgcgcg
     7501 aaccagcggt gctccgtttc ctgcacctgc cggataacca gggaaaggtt ggtgaactgc
     7561 aggtccgcta tcggcgtgaa agcgggcgcc ttggtcactc cccgtcttcc ttgcgcgtca
     7621 tgaaagcctc aacttcagcc tccatcagct cccgctcggt cggcggaagc agctggacgt
     7681 agtcctcggg ccggtcgccg ccgcgcatct cagccggggc aggttcccgg tcgtcaacag
     7741 gctcagggtt ggtcatggcg tcacgctacc tcagtagtgc acgtgataga cagagggcca
     7801 cacctcggcc gtcgcctccg ggacgtgcac atactgcacc ccggccgccg tccagcgcga
     7861 caccagctcc cagtcctcga ccgagctggc cgggccccag gtgccgtgct ccagcgtctc
     7921 gcggcggtgc atgatcatcg gggtgcccag gttgccgcac gccagctgcc cgtggccgat
     7981 ttccgtgcac agccccgagc tggaccagct ccgcatgacc gagtacgccc acccggcgcc
     8041 cgggttctgg gtcagggccg ccgccagctt ggcgcagtgc tccggcatca gcaggtcgtc
     8101 gtcatcgatg taaccgatgt aagccccgcc gcttgcctcg atcccggcca gccgcgcgag
     8161 gtgaccccag tgctgctccg ggctgtgctc ggcaagctgg acgaaccgga ccgggtggta
     8221 gaaggccccg ctctcgtgca gcttgccgat cttctccagc agctccgggt ccggcccgtc
     8281 ggagacgatg acatgctcgg tgttccgcca ggtctgtgcc tgcaccgacg ggatgcagcg
     8341 gttcagcagc cgctcgtgcc gcccccaggt cggggtgatg atgctgacgc gggggatcac
     8401 cacggccaca cttccgggat attcaggccc cggttgaact cggccagttc ctggtcactg
     8461 tacggccggc cgccgagctt gccgatgtcc tcgttcagct ggcccgagcg gcagtaggcg
     8521 tgccacgcct gaaggtcggc acccgtcccc gcgtaggaat tctggtaggt ggcgtcgttc
     8581 ccggagcggc cggtggtgta gtgcaggtgc gggatcagca cgtcatgcag gtacgtgatc
     8641 ccgcaggccc ggccccaggc catccacgcg acgtccacat acatgtgcgc tatcgacggc
     8701 ggcccgaagt accccagcgt gcggaccacc tcgctgcgca tgaagacatg gcagcacagg
     8761 gtgccgggct cgcggccggg gtacaggtcg ttggcgaacg cgaacggtgt ctgctccagg
     8821 gcctccatga tcttcacgtc ccagcccggc gtctccggca cgttgtcgtc cccgaagtgg
     8881 ccgatgtacc ggaaggcggt cgcctccggg actgccaggt ggttgcacca ggctgtgacc
     8941 ttgtgcaggt ccgcttccat cgtgtaggtc agccgcttgc ccacggggta gtgctcagcc
     9001 gtggggtcgt cgttgtcgag gccgatctcg aaccaggtgt caccctcgca ggtcgcgtcg
     9061 gcggcgtcac tcagccgtgc gacgtttccc ggccgtcccc ggctggggat gaccatcagc
     9121 aggtctctca tgcatacggt cctatctggc gcagccacgg gtacgacggc a
//
LOCUS       DASZYN010000595         4660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_350831, whole genome shotgun sequence.
ACCESSION   DASZYN010000595 DASZYN010000000
VERSION     DASZYN010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350831"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VHE33_15435"
     CDS             <1..896
                     /locus_tag="VHE33_15435"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HVT98897.1"
                     /translation="DAAEVYVKAEGLQPIGAFKIRGAYNKVAQLTPDERSRGVITYSS
                     GNHAQAVAYSARAFGAKAVIVMPSNAPEVKKRATAALGAEIVEVGNASSDRLRKAEEL
                     QAAHGYIMIPPYDDDQIIAGQGTCGLEILEDLPDVDLVLSPVSGGGLLSGIATAIKLS
                     NPRTPVQTRVIGVEPELAADAQESFRTGHVVTWPADKTTRTLCDGLRTQSLGERNFEH
                     IRAYVDDIITVTEAEIRSAMRTLLHSARLTPEPSGACSAAAILFHRDQLLPFRKAVVV
                     MSGGNVEPQVLESVLAEKDPQ"
     gene            1100..2101
                     /locus_tag="VHE33_15440"
     CDS             1100..2101
                     /locus_tag="VHE33_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98898.1"
                     /translation="MIRPALAFLLLLTAAAAFGQKPLPRQQMPRNGTLATLVREANLY
                     ANPDDSAGPISSILPGRELVIAEKSGKWLRVFANTDAPDTRQADVPVFGTEGQAQPIS
                     GWMLNKGIVDINTPHGDSILFGTADDLEQQAAVSNPPPHVAEEARLLYRRITTMYPDS
                     PWTAEAMYRAADIRWQLQKADASTLPSAHEKENYLREQMDEEEMKKILKYFPNTRWAD
                     LAAFDLIDNKLCGDWQGSEKCPEQETNYYLKYADEHPDSPKAAQALYNAVWRQAAAGD
                     MWTADGNGKRADEDRGHAKAIFDRMQQKFPQSSYTARAASLIFKIDQGVPVYGSSRE"
     gene            2215..3189
                     /locus_tag="VHE33_15445"
     CDS             2215..3189
                     /locus_tag="VHE33_15445"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HVT98899.1"
                     /translation="MHRTAYLVTIANDPAVLPQAGARLNDYVISVLLGIIEGLTEFLP
                     VSSTAHLRIGEALFDISLDDPYWKMYTIVIQLGAILALTFLFTGRIIEFVRTFPRGED
                     GKHTIRNHPLTLTLVAFVCTAIPALALTPVIGRHLESLRVMALSLIIGGIVMWIVDAW
                     SVRRDLPVSDVEQMSLPQSIWIGLCQVTSAVFPGTSRSMSTIAAGQLAGMTRPAALEF
                     SFLVSIPTMFAATIYDLLKALFPGLHLLHRHHGAATASHAALAPLAMTGHGWLVLIVG
                     FLVSFFVALGVVEWFLQWVRTHGFVLFAVYRLLLGAAIFIFGARMMAN"
     gene            complement(4193..>4660)
                     /locus_tag="VHE33_15450"
     CDS             complement(4193..>4660)
                     /locus_tag="VHE33_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98900.1"
                     /translation="KTLKSHWLGNEWVFVMVNTKTILVLSVIALACAVTCLPARAQNT
                     CSGVDRTLSKERAEAFEPSVARQLRAKQVDIMQSFRFRGWSILYVDPHQSEEVFVFYS
                     SNPMRTNYVTLWSGAAREDEQDQIRAWTIKNAPGIPHALANCFAWHVTRDRDK"
BASE COUNT          908 a         1676 c         1196 g          880 t
ORIGIN      
        1 tcgacgccgc agaagtttac gtcaaggccg aaggcctgca gcccatcggc gccttcaaaa
       61 tccgcggagc ctacaacaaa gtcgcccagc tcactcccga cgagcgttcc cggggagtca
      121 ttacctactc ctccggcaat cacgctcagg cagttgccta ctcggcgcgc gccttcggag
      181 cgaaagccgt catcgtcatg ccttcaaacg cccccgaggt taagaaacgc gccactgccg
      241 ccctcggcgc cgagatcgtc gaagtcggca acgccagttc cgaccgtctt cgcaaagccg
      301 aagaactcca ggcagcgcac ggctacatca tgatcccgcc ctacgacgat gaccagatca
      361 tcgccggcca gggcacctgc ggcctcgaaa tcctcgaaga cctccccgac gtcgacctcg
      421 tcctctcgcc cgtctccggc ggcggactcc tcagtggcat cgccaccgct atcaagcttt
      481 ccaacccccg cacgccagtt cagactcgcg tcatcggcgt cgagcctgaa ctcgccgccg
      541 acgcccagga gagctttcgc accggccacg tcgtcacctg gcccgccgac aaaaccaccc
      601 gcaccctttg cgacggcctc cgcacccagt ccctcggcga gcgcaacttc gagcacatcc
      661 gcgcttacgt cgacgacatc atcaccgtca ccgaagccga aatccgctcc gccatgcgca
      721 cgctgctcca ctccgcgcgc ctcactcccg agcccagcgg cgcctgctcc gccgccgcca
      781 tcctcttcca ccgcgaccag cttctcccct tccgcaaagc cgtggtcgtg atgagcggcg
      841 gcaacgtcga gccgcaggtg ctcgaatcgg tcctcgccga gaaagacccg caatgacgca
      901 ggcacgaaag aaaccctgtc ttgaaagggc accgcttcag ccgtgccgga atgctgacga
      961 tcctcatgcc cgttctcgct ttgaaggggc acggcttcag ccgtgccgtt acaaccagca
     1021 gattgatccg gctttagccg ccgaggaatg gttctctttc gctgtgagac gaaccggccc
     1081 tttttcacgt cctatctctg tgatccgtcc agccctcgcg ttcctcctgc tcctgaccgc
     1141 ggccgccgcc ttcggccaaa aaccgctgcc ccgccagcag atgccccgca acggcaccct
     1201 ggccaccctg gtccgcgaag ccaacctcta cgccaacccc gacgactccg ccggccccat
     1261 ttccagcatc cttcccggcc gtgagctggt catcgccgaa aagagcggca agtggctccg
     1321 cgttttcgct aacaccgacg cgcccgacac ccgccaggcc gacgttcccg tcttcggcac
     1381 cgaaggccag gcccagccca tctccggctg gatgctcaac aagggcatcg tcgacatcaa
     1441 tacgccccac ggcgactcca tcctcttcgg cactgccgac gatctcgagc agcaggccgc
     1501 tgtctccaac ccgccccccc acgttgccga ggaagcccgc ctcctttacc gccgcattac
     1561 cacgatgtat cccgactcgc cttggactgc tgaggccatg taccgcgccg ctgacatccg
     1621 ctggcaactc cagaaggccg atgcctccac cctcccctca gcgcatgaaa aagaaaacta
     1681 cctccgcgag cagatggatg aagaggagat gaagaagatc ctgaaatact tccccaacac
     1741 ccgctgggcc gacctcgccg ccttcgacct gatcgataac aagctctgcg gcgactggca
     1801 gggatcggaa aaatgccctg agcaggagac caattactac ctgaaatacg ccgacgagca
     1861 ccctgactca cccaaagccg cccaggcact ctacaacgca gtctggcgtc aggccgccgc
     1921 cggcgatatg tggaccgccg acggcaacgg caaacgtgcc gatgaggatc gcggccacgc
     1981 caaagccatc ttcgatcgca tgcagcagaa attcccgcag tcctcgtaca cggcccgcgc
     2041 cgccagcctc atcttcaaaa tcgatcaggg cgttcccgtc tacggctcca gccgcgagtg
     2101 aggactgtcc cattttcaga cacccggcct cgcgcccgta cccacgatgc cacctgctaa
     2161 cgtgaatttc cccgcgcgtc cgccgttctc tgcccaggaa ctctgctata caaggtgcat
     2221 cgcaccgcat acctcgtaac gatcgcgaat gaccctgcgg ttctccccca ggccggagca
     2281 agattgaacg actacgtgat ctctgtactg cttgggatca tcgagggcct caccgaattc
     2341 ctgcccgtca gttccaccgc tcatctgcgc attggcgaag ccctcttcga catcagcctc
     2401 gatgacccct actggaagat gtacaccatc gtcattcagc tcggcgccat cctcgccctc
     2461 acgttcctct tcaccggccg catcatcgag ttcgtccgta cctttccgcg cggcgaagac
     2521 ggcaaacata cgattcgcaa tcacccgctg actctgacgc tcgttgcctt tgtctgcacc
     2581 gcgattcccg cgctggctct cactccggtg attggcaggc atctggaaag cctccgcgtt
     2641 atggccctgt ccctcatcat cggaggcatc gtcatgtgga tcgtcgacgc ctggagcgtc
     2701 cgtcgcgacc tccccgtctc cgacgtcgag cagatgtccc ttccgcagtc gatctggatt
     2761 ggcctttgcc aggtcacctc cgccgtcttt cccgggactt cgcgctctat gtccaccatc
     2821 gccgccggtc aactcgccgg catgacccgc cctgcggcgc tcgagttcag cttccttgtc
     2881 tcgattccga ccatgttcgc ggcgacgatc tacgacctgc tcaaggccct ctttcccggc
     2941 cttcatctcc tgcaccggca ccatggcgct gcaaccgcct cccacgcggc gctcgccccg
     3001 ctcgccatga ccggacacgg atggctggtc ctcatcgtcg gcttcctcgt ctcatttttt
     3061 gttgcgctcg gcgtcgtcga gtggtttctg caatgggttc gcacccacgg cttcgtcctc
     3121 ttcgccgtct accgcctgct gctgggagcc gcaattttca tctttggcgc acgcatgatg
     3181 gcaaactgac agctttgatg aatagggtga accactcacc gtgatctggg catatattcc
     3241 tgcggtccgc gccaatggcg acggcaccct caccgatgtg accgcaaaag ccgactttca
     3301 accgggccgc aagtacgctg ggaatgacgc ggcttcggcc gccggggatg ttcttcctcg
     3361 ccggtgcccc ctgtctgctc gggttacaga taggggagcc gggaatctca atccagccgc
     3421 cgttctgtca gggcaggact ctcgagccac cgcaaaaaaa gtcgcgccgc caggcgcaac
     3481 cggaatcccc gacacggccg gggtgggatc gaaccgcggc cgcgctcccg cagcaattcc
     3541 cctccaccca aacagcgccc cgaagcgtcg cgccagcggc gccccggtag aagcgccggg
     3601 ttttaacccg gcgaaaaccg cgcccatcca cacggcggct tcagccgcgg cactttccgt
     3661 tcgaccggca ttgttcgaaa cttccgtgtt gccgactact ctggatgttc ggcgagagat
     3721 tcggtggttg aaggctcgaa gattacacaa tgaggcatcc agcagtcggt tttcttcgtc
     3781 gcaacctcgg cctcacaatg aacgcatagc agatttgggc cgtccattcc atcgagaccg
     3841 caacagccgg aaagcctccg ctgcacagtc gtcaggtgcg tatttacaac gtctgcgatg
     3901 ttcagaatcc agccgccaac gttctggtgg aagaatgagt cgtcttcctg gatcgccgca
     3961 cctggcggga cgtaggattc ccgatcattc gtattccgct cgtcggcagt cgctaggcgc
     4021 acatcctccg acaagcgagt cttgcaatgc gcgcacctaa cgtgcatcga aatcggcgcc
     4081 cctttcaata atggacgaaa aatcggctac acggacattt cgcatccgaa acggccggaa
     4141 ggccgacgat tctgccaaat ttccccaacg ccgaggagac agaaagtaac gctcacttat
     4201 cgcggtcccg agttacgtgc cacgcaaagc aattggcgag tgcatgggga atgcccggag
     4261 cattcttaat agtccaggcg cgtatttgat cttgctcatc ctcgcgggcc gcgccactcc
     4321 acagcgtgac gtaattggtt cgcatcgggt tcgaggaata gaagacaaac acctcctcag
     4381 attggtgcgg atcgacgtac aggatgctcc aacctctaaa tcgaaacgat tgcataatgt
     4441 cgacctgctt tgcgcgaagc tgccttgcaa cagacggctc aaacgcctcg gcgcgctcct
     4501 ttgagagcgt cctgtcgaca cctgagcagg tgttctgtgc gcgggccggc aggcatgtaa
     4561 cggcacatgc cagagcgatc accgacagca ccaggattgt cttcgtgttc accatgacga
     4621 aaacccactc atttccgagc cagtgcgatt ttagcgtctt
//
LOCUS       DASZYN010000596         2152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_35030, whole genome shotgun sequence.
ACCESSION   DASZYN010000596 DASZYN010000000
VERSION     DASZYN010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35030"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1257)
                     /locus_tag="VHE33_15455"
     CDS             complement(79..1257)
                     /locus_tag="VHE33_15455"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HVT98901.1"
                     /translation="MTNLKKQKTYINSDLQRAVAASESQVLDQIRRLAEIESPSDDKP
                     SVDRCAEAVADLANSLGAKVRRHRQRAFGDVLELRFESGAKGKSRLAPVMLLGHLDTV
                     WPLGTLSGMPFRIEKGRAWGPGVLDMKAGVVMGLTAIEALRQTGSLGRPVVFLLSGDE
                     EVGSTVSRPIIEKVARQCAAVFVLEPAQGLDGAYKTARKGVGDYTLRVKGVAAHAGVD
                     FQKGHSAIHELAWQLEQVRGFTELDRGLTVNAGVIRGGTRTNVIAAEAEAEIDVRIAR
                     APDAARIDRKFRALRVRDRSCVLHVEGGMNRPPMERSRGTVALYRRAATLAAEMGFSL
                     AEAATGGGSDGNFTAALGVPTLDGMGAVGEGAHASHESVLLNQLVPRTALLAEMIRTT
                     "
     gene            1368..>2152
                     /gene="lpxC"
                     /locus_tag="VHE33_15460"
     CDS             1368..>2152
                     /gene="lpxC"
                     /locus_tag="VHE33_15460"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HVT98902.1"
                     /translation="MAESSHLENTISSPIEFAGVGLHSGAPVRMRLLPAVAGSGVVFR
                     RTDLDGFEIPATGRNVAKVSYATSLMRQGVLISTTEHLLSALLGCGVDNVVVEVDNLE
                     LPILDGSALPYTQAFREVGLKMQKRRREYLRILKDVEVREGDKFIGVYPGSGYSVSYK
                     IEFPAPIGRQGYTVDLATGDYEREIAPARTFGWREDEKRLRDMGLIRGATEESVIVLS
                     RQGVLNGNLRFRDEFVRHKVLDLIGDLALAGHHILGHVVAERAG"
BASE COUNT          397 a          690 c          625 g          440 t
ORIGIN      
        1 gccatgatca agccgaggac aatcaggatt gtcaccgcca gcagcgcgat ctccatcagc
       61 ttcagcatcg gaatcccctc aggtggttcg aatcatctcc gccagcaaag cggtgcgcgg
      121 caccagttgg ttgagcagta ccgactcatg agatgcatga gcaccttcgc cgactgcacc
      181 cattccatcg agtgtcggca ctccaagcgc tgcagtgaag ttaccgtcgg aaccaccgcc
      241 ggtcgcagct tccgccagtg agaaccccat ctccgcggcc aatgttgcgg cacgccgata
      301 cagcgcgacg gtgccgcggc tccgttccat gggcgggcga ttcattccgc cctccacatg
      361 caggacgcag gaacggtcgc gaacgcgcag agcccggaac ttcctgtcga tacgcgccgc
      421 atccggcgcg cgcgcgatgc gcacgtcgat ctccgcttcg gcctcggctg caatcacgtt
      481 ggtccgcgtg cctccgcgga taaccccggc gttcacggtc aggccgcgat cgagttcggt
      541 gaacccgcgc acctgttcca gttgccaggc caattcgtga atggcgctgt gtcccttctg
      601 gaaatccacc ccggcatgcg cggccacccc cttcacgcgg agcgtgtagt cgccgactcc
      661 cttgcgcgcc gtcttataag cgccgtcgag accctgcgcc ggttccagca cgaagaccgc
      721 cgcacactgg cgagccactt tctcgatgat cggccttgaa accgtgctgc ccacttcttc
      781 atctccgctg agaagaaaaa ccaccggccg ccccagactg ccggtttggc gcaacgcctc
      841 gatcgcggtc agccccatca cgactcccgc tttcatatcg aggacgcccg gaccccacgc
      901 gcggcccttc tcgatccgaa acggcatgcc ggatagtgtc cccaggggcc agaccgtatc
      961 aagatgaccg aggagcatca ccggcgccag ccggctcttc cctttcgcac cggattcgaa
     1021 gcgtagttcc aggacgtcgc cgaatgcgcg ctggcgatgg cgccgcacct tggccccaag
     1081 tgaattcgcc agatcggcga ctgcttcagc gcatcgatcg acgctcggtt tgtcgtcgct
     1141 gggtgactca atttcggcca gacgacgtat ctggtcgagc acctgggatt cagatgccgc
     1201 tacggctcgc tgcagatcgc tgttgatata agtcttttgt tttttaagat ttgtcatgat
     1261 ggttgcctga tcaggaacta ttactttcga taccagagtg tggcgatttt gccacactac
     1321 aactcttcat ttccggttgt agtctatgtc ttcattgagg acaccgcttg gctgaatcat
     1381 cgcacctgga aaacacaatt tcctccccga tcgagtttgc cggcgttggc cttcacagcg
     1441 gcgcaccggt tcgtatgcgc ctgctgccgg cagtggctgg ttcgggcgta gttttccggc
     1501 gcaccgatct ggatggtttt gagatccctg ccacgggccg caacgtcgct aaagtcagct
     1561 atgccaccag cctgatgcgg caaggcgtgc tcatttccac gaccgaacat cttctctccg
     1621 cgctgctggg ctgcggtgtc gataacgttg tggtggaagt ggataatctc gagctgccga
     1681 tccttgacgg cagcgctctg ccctatacgc aggccttccg ggaagttggg ctgaagatgc
     1741 agaagcggcg ccgggagtat ctccgcattc tgaaagatgt ggaagtgcgc gagggcgaca
     1801 aattcatcgg agtctacccc ggcagcggct actcggtttc ctacaagatt gaattccctg
     1861 ccccgatcgg ccgccagggc tacacggtgg atctggccac cggggattac gagcgcgaga
     1921 ttgctccggc tcgcacgttt ggctggcgcg aagacgaaaa gaggctgcgc gatatggggc
     1981 tgatccgcgg cgccacggaa gaaagcgtga tcgtgctgag ccggcagggt gtgctgaacg
     2041 gcaatttacg cttccgcgac gagtttgttc gacacaaagt actcgacctg atcggcgacc
     2101 tggccctggc cgggcaccac attctcggcc atgtggttgc cgagcgcgcc gg
//
LOCUS       DASZYN010000597         6902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_35042, whole genome shotgun sequence.
ACCESSION   DASZYN010000597 DASZYN010000000
VERSION     DASZYN010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35042"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="VHE33_15465"
     CDS             complement(<1..1382)
                     /locus_tag="VHE33_15465"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HVT98903.1"
                     /translation="MNNDLVEELERRNRLAEEGGGEERRRKQHSEGKLGARERIDLLL
                     DDGTFVETDRFVTHRATDFGMGDKRFLGDGIVTGYGKIDGRTVFVFAQDFTVFGGSLS
                     EANAAKIVKLMEMALKVGAPVIGLNDSGGARIQEGVVSLAGYADIFLRNTMASGVIPQ
                     ISAILGPCAGGAVYSPAITDFILMVDKTSYMFVTGPDVIKTVTHEEVTKEALGGAATH
                     NEISGVAHFEAHDDAECLTMVRELLSFLPSNNLDDPPRRPCTDPVDRADRELDTLVPL
                     DSTQPYDVKKAITHIVDDGYFFEVHEHFAMNVVVGFARMGGRPVGILANQPAVLAGVL
                     DINASVKGARFVRFCDAFNIPLITLEDVPGFMPGTQQEYGGIIRHGAKLLFAFAEATV
                     PKMLVITRKAYGGAYCVMSSKHLRTDVNLAWPTAEIAVMGPEGAVNIVYGRELAHTVK
                     LAEKSEGPLS"
     gene            complement(1442..1972)
                     /locus_tag="VHE33_15470"
     CDS             complement(1442..1972)
                     /locus_tag="VHE33_15470"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HVT98904.1"
                     /translation="MNWTKLLKMEIDSTYRVTARLLERVTDESLEWKPEAGSNWMTMG
                     QLIQHLATGCGWGCRGFVTGEWGLPEGKTWADLTPEENLPPAEKMPASASVAEGKRLL
                     EEDRVLALAMVDRAGEERLALEAAATPWAPGVKRPLGVQLLRMVQHLDRHKSQLFYYL
                     KLEGVPVNTTDLWDGP"
     gene            complement(2059..2511)
                     /locus_tag="VHE33_15475"
     CDS             complement(2059..2511)
                     /locus_tag="VHE33_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98905.1"
                     /translation="MQRIFILTMAAGLGIAPAFGETYKTPFSVACGEVWPAVKATLAD
                     QEHYAKVIINDETMKADYQPKHTVHVDISGTLLQRMNHVKLVPKDTGCEMQVVSNYSG
                     WGHNDEGDFRKRVADNMTKAKTPGAEPAAAPATEPVKPGTTDEAQKPQ"
     gene            complement(2826..3449)
                     /locus_tag="VHE33_15480"
     CDS             complement(2826..3449)
                     /locus_tag="VHE33_15480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HVT98906.1"
                     /translation="MTSANDSLNLTVALTGASGIVFGQMLLRALDADARVGRIHFVPS
                     ENSLRVLAEELGISGRTGLVEKLLDAPSAKIVQHSESDIGAAIASGSYPSAGMIVLPC
                     SMGTLAGIANGMAGNLIERAADVCLKERRPLILCLRETPFNKIHIRNMGLAADAGATI
                     FPVIPAFYNRPVDSNEMARQFVMRVLGHMGLPQAGAYAWKADDANAG"
     gene            complement(3499..3942)
                     /locus_tag="VHE33_15485"
     CDS             complement(3499..3942)
                     /locus_tag="VHE33_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98907.1"
                     /translation="MRKFLPAVLLLAFSLPLLAQGAPPQGGADQPYAMEYYYKTQWGH
                     QQEFLDLFLKYHYPLLQRIEKTGRMLSVKIETPANHMTEDARWDYRVTIVFKNSTVAT
                     TANPDEPALIKELWPDQADYQAHEQRRFEILLAHWDLPVSNITPK"
     gene            complement(4389..5696)
                     /gene="purB"
                     /locus_tag="VHE33_15490"
     CDS             complement(4389..5696)
                     /gene="purB"
                     /locus_tag="VHE33_15490"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HVT98908.1"
                     /translation="MIPRYTRPAMGRLWTDESKYRAWLEVESVASEILAEDGIVPVEA
                     AKAIRERGDFSIERIQQIEAEVKHDVIAFTTAVAEKVGAESRWLHYGLTSNDVVDTAQ
                     ALQIKAASALLGEDLSRLLAVLKARALEFKHTPTVGRTHGQHAEPTTFGLKLLNWYAE
                     MRRNLERFEAAAEQMRVGKLSGAVGAFGHISPRQEERICARLGLEPALVATQVIQRDR
                     HAYYIATLAVITATLDKIATEIRHLQRTEVREVEEYFSEKQKGSSAMPHKRNPITSEQ
                     ISGLARVVRANAQAALENVALWHERDISHSSVERVILPDSTILTDYLLAKTTDLIEKL
                     VVYPARMQKNLDSTGGLIFSGQLLLDLAEAGMLREDAYRLVQRHAMNAWQNDLVFRDL
                     VAGDAEIAQRLSAEKLARTFDLGRQLSNVDAIFARVLGSGNGA"
     gene            complement(5772..6236)
                     /locus_tag="VHE33_15495"
     CDS             complement(5772..6236)
                     /locus_tag="VHE33_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98909.1"
                     /translation="MDAGHCLAADHRDWLNVASEKPYEVELGLAAPEKADSGKAPIYL
                     IQYTTPTHTEGVAFAFVSRGKDSHRKLVPFVGGGQDPHRDLVLQFQTQFHQTDDGSQK
                     VSLVDPPLGGVATQEEILAAIQQVGFHTWRVPVANLRNYSGSAQCKLAEAAK"
     gene            6402..6635
                     /locus_tag="VHE33_15500"
     CDS             6402..6635
                     /locus_tag="VHE33_15500"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HVT98910.1"
                     /translation="MAEVVTVASFTEPLEAEMAKLRLESAGIEVFLSGENARIMEPGL
                     GPLQLQVSSDDEADARAILADPGEPAGQDSQIS"
     gene            6676..>6902
                     /locus_tag="VHE33_15505"
     CDS             6676..>6902
                     /locus_tag="VHE33_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98911.1"
                     /translation="METAEIHGAISLARHGQRSDPGKEPQPLTRAQMRRLAEFRFQLR
                     KFLHFSSLAAGAAGVRTQQYQLLQCIWGMPE"
BASE COUNT         1308 a         2506 c         1746 g         1342 t
ORIGIN      
        1 tcgctcagcg gtccctcgct cttctccgcc agctttacgg tgtgggccag ctcacgcccg
       61 tacacgatgt tcaccgcgcc ttccggtccc atcaccgcga tttcggccgt cggccatgcc
      121 aggttcacgt ccgtccgcag atgcttcgag ctcatcacgc agtaagctcc gccatacgcc
      181 ttgcgcgtga tcaccagcat cttcggcacc gtcgcctctg caaaagcaaa cagcagcttc
      241 gctccgtgcc gaatgatccc tccatactcc tgctgcgtgc cgggcatgaa ccccggcaca
      301 tcctccagcg tgatcagcgg aatgttgaac gcgtcgcaga aacgcacgaa ccgcgcccct
      361 ttcacgctgg cattgatgtc cagaaccccc gccagcactg ccggctgatt cgccagaatc
      421 cccaccggcc tcccgcccat cctcgcaaat cccaccacca cgttcatcgc gaagtgctca
      481 tgcacctcga agaaataccc atcgtcgaca atgtgcgtga tcgccttctt gacgtcgtag
      541 ggctgcgtcg aatccagtgg caccagcgtg tccagctccc ggtccgcgcg atccaccgga
      601 tcggtgcacg gccgccgcgg cggatcgtcc aggttattcg acggcaaaaa gctcagcagc
      661 tcgcgcacca tcgtcaggca ttcggcatcg tcatgagcct caaaatgcgc gacgccggaa
      721 atctcgttgt gcgttgccgc tccccccagc gcctccttcg tcacctcttc atgcgtgacc
      781 gtcttgatga cgtccggccc ggtcacgaac atgtacgacg tcttgtccac catcaggatg
      841 aagtccgtaa tggccggcga atacaccgcg ccgcccgcgc acggccccag gatcgccgaa
      901 atctgcggaa tcactccgct cgccatcgtg ttccgcagaa aaatgtccgc gtatccggcc
      961 agcgaaacca ctccttcctg aatcctcgcc cctccggaat cgttcagccc gatcaccggt
     1021 gcgcccacct tcagcgccat ctccatcagc ttcacgatct tcgcggcgtt cgcctccgag
     1081 agcgaccccc caaacaccgt aaaatcctgc gcgaacacaa acaccgtccg cccatcgatc
     1141 ttcccgtatc cggtgacgat cccatccccc aggaagcgct tgtcccccat cccgaagtcc
     1201 gtcgcgcgat gcgtgacgaa ccggtccgtc tccacaaacg tcccgtcgtc cagcaacaga
     1261 tcgatgcgct cccgcgcgcc cagcttcccc tcgctgtgct gcttccggcg ccgttcctcg
     1321 ccgccgcctt cttccgccag ccgatttcgc cgttccagtt cttccaccag atcgttgttc
     1381 atcgcctcgg attatagcgg tcccaacaca cacctctcgt gctcgccctc acacccgttc
     1441 ctcacggccc atcccacaaa tccgtcgtgt tcacaggcac gccttcgagc ttcagatagt
     1501 agaacaactg gctcttgtgc cgatcgaggt gctgcaccat gcgcagcagt tgcaccccta
     1561 acggcctttt cactcccgga gcccacggcg ttgctgccgc ctccagcgcc agccgctcct
     1621 ctccggcgcg gtccaccatg gcaagcgcca gcacccggtc ctcctccagc agcctcttcc
     1681 cctccgccac actcgcgctc gccggcatct tctcggccgg cggcaaattt tcctccggcg
     1741 tcaaatccgc ccaggtcttt ccctccggca gcccccactc cccggtcacg aacccccggc
     1801 aaccccatcc gcaccccgtc gcaagatgct ggatcagctg tcccatcgtc atccagttcg
     1861 atcctgcttc cggcttccac tccaggctct cgtccgtcac ccgctccagc aggcgcgccg
     1921 tcacgcgata cgtgctgtcg atctccatct tcagaagttt cgtccagttc atgggccctc
     1981 cttgcttggg cacacccgca cacaagtcgc cggcaggcgg attcgcgatc cgctccttgc
     2041 tgtcggcctt tcgctgccct actgcggctt ctgtgcctcg tcggttgtcc ccggcttgac
     2101 cggctcggtt gccggagccg cagccggttc cgcacccggc gtcttggcct tcgtcatgtt
     2161 gtccgccaca cgcttcctga agtcgccttc gtcgttgtgt ccccagccgc tgtagttcga
     2221 caccacctgc atctcgcatc ccgtgtcctt gggaaccagc ttcacatggt tcatccgctg
     2281 cagcagcgtg ccgctgatgt ccacatgcac cgtgtgcttc ggctggtaat ccgccttcat
     2341 cgtctcgtcg ttgatgatca ccttcgcgta gtgttcctga tctgccagcg ttgccttcac
     2401 cgccggccac acctcaccgc acgccaccga aaacggtgtc ttgtaggtct cgccaaacgc
     2461 cggcgcgata cccaaccccg cagccatcgt cagaataaaa atgcgctgca acaatgtgct
     2521 acctccctcc gctacgcgga gattccccag gcagccaggt ccgtcgcacg cgctccgatt
     2581 cctcaggttc cagcgcgcat cggagccaac accatccggg caacaaggaa cgccgagccg
     2641 ggcgacttgc aggattttct gcccgccatc ctactcccca tagcggacaa tacaagcccc
     2701 tccggtctgc tctctcaggg cccagcatct gcgtgttttt gccagatggg cacgaagcct
     2761 cgattgtcga cattttcgat tgtgagcttt ggaagagtgg tcaggatctt ctgcaacgca
     2821 ctacgtcagc ccgcattcgc gtcgtccgcc ttccaggcgt acgcccccgc ctgcggcagc
     2881 cccatgtgcc cgagaacgcg catcacaaac tgccgcgcca tctcattcga gtcaaccggc
     2941 cggttgtaga acgccggaat caccggaaaa atcgtcgccc ccgcgtccgc agccaggccc
     3001 atgttgcgaa tgtgaatctt gttgaatggc gtctcccgca gacacaaaat gagcggccgc
     3061 cgctccttca aacacacatc ggccgcgcgc tcgatcagat tgcctgccat cccattcgcg
     3121 atccccgcca gcgtccccat actgcacggc aacacgatca tccccgctga gggataactc
     3181 ccgctggcga tcgccgcgcc aatgtcgctc tcgctgtgct gcacgatttt cgccgaaggc
     3241 gcatcgagca gcttctcgac caggcccgtt cgccccgaaa ttcccagttc ctccgccagc
     3301 acccgcagcg agttttccga gggaacaaaa tggatgcggc ctacacgcgc atccgcatcg
     3361 agcgcgcgca gcagcatctg cccgaagaca atgcccgagg cgcccgtcag cgcgaccgtc
     3421 aggttcagcg aatcgttcgc ggaagtcacg aagagattgt aggggatcgg acctgccgct
     3481 cctgcttcca ccttccagct acttcggcgt gatgttgctc accggcaaat cccagtgcgc
     3541 cagcaggatc tcgaaccgcc gctgttcgtg ggcctgatag tcagcctgat cgggccaaag
     3601 ttctttgatc agcgccggtt cgtcaggatt cgccgtcgtc gccacggtcg agttcttaaa
     3661 gacaatcgtc acccgatagt cccagcgcgc atcctcggtc atgtggttcg ccggcgtctc
     3721 aatcttgacc gacaacatcc gccccgtctt ctcgatccgc tgcagcagcg gatagtggta
     3781 cttcagaaac aaatcaagga actcctgctg atggccccat tgcgtcttgt agtagtactc
     3841 catcgcatac ggctgatcgg ccccaccctg cggcggcgcg ccctgcgcca gcagcggcag
     3901 tgaaaaggca agaagaagca cagcgggaag aaactttctc atggagatcc cccggccaga
     3961 gtttggcccg attgtaagcc tccggacccg cctgtccggt cagaatccga aaatccctga
     4021 cctcaacggt cgttttgaat caagcttcag aggcctgcaa aaaagacagt gccccccgcc
     4081 gctggatcgg ccaggttcac cccacagcaa gtagcgaaag ctcccgccat gcgaaagtca
     4141 acaaaaactc gaccctccgc cctgccaaaa gtactgtcct ggaccgtttt gggaaaaacc
     4201 gccgtcccac ctccggcctc tcgactttcc cgcaaactac tagcaggctg ccgaaacagt
     4261 cctgtcccgc aagccgttgc agcgtgcgga aaactcgccg cctcaaggga cacagctatg
     4321 ccaaaagggc tgtaaaacca aaccggcgtt agatcctaag gaacgagctc caggattgca
     4381 ccgattcttc aggcgccgtt tccgctgccc agcacgcgcg caaaaatcgc atccacattc
     4441 gaaagctgcc gcccgagatc gaacgtccgc gccagctttt ccgcgctcag ccgctgcgca
     4501 atctccgcat cgcccgcaac cagatcgcga aacaccagat cgttctgcca cgcgttcatc
     4561 gcatgcctct gcaccaggcg ataggcatcc tcccgcagca tgcccgcctc cgcaagatcc
     4621 agcagcagtt gcccgctaaa gatcagcccg cccgtcgaat cgagattctt ctgcatccgc
     4681 gctggataca ccaccagctt ctcgatcaga tccgtcgtct tcgccagcaa atagtccgtc
     4741 agaatcgtcg aatccggcag aatcacccgc tcgaccgaag agtgcgaaat gtcccgctcg
     4801 tgccacagcg cgacattctc caacgcggcc tgcgcgttcg cccgcaccac gcgcgccagc
     4861 ccgctgatct gctccgatgt gatcgggttc cgcttgtgcg gcatcgccga cgagcccttt
     4921 tgcttttcgg agaaatattc ctccacctcg cgcacctcgg tccgctgcag gtggcgaatc
     4981 tccgtcgcaa tcttgtccag cgttgctgta atcaccgcca gcgtcgcaat gtaatacgcg
     5041 tgccgatccc gctggatcac ctgggtcgcc accagcgccg gctccagccc cagccgcgcg
     5101 caaatccgct cctcctgccg tgggctgatg tgcccaaacg cccccaccgc ccctgaaagc
     5161 ttccccaccc gcatttgctc ggcggccgct tcaaaccgtt ccaggttgcg ccgcatctcc
     5221 gcataccagt tcagcagctt caggccgaag gtcgttggct ccgcgtgctg cccgtgcgta
     5281 cgccccaccg ttggcgtgtg cttgaactcc agcgcccgcg ccttcagcac cgccagcagc
     5341 ctggatagat cctccccaag cagcgccgac gcagccttga tctgcagcgc ctgcgccgta
     5401 tccaccacat cgttcgacgt cagcccgtag tgcagccagc gcgactccgc gcccaccttc
     5461 tccgcaaccg ctgtcgtgaa agcgatcacg tcgtgcttca cttcggcttc aatctgctgg
     5521 atccgctcga tcgaaaaatc gccgcgctcg cgaatcgcct tcgctgcctc caccggaacg
     5581 atgccgtcct cggcgaggat ttcgctggcc acactctcca cttcaagcca ggcacggtat
     5641 ttgctttcgt cagtccaaag gcggcccatc gccgggcgcg tatagcgtgg aatcaaagct
     5701 gagatctcca ggagaactca tcattctatc ctgcgaaaat cgcaccggcc ccgccgcttc
     5761 gactcgccgc tttacttcgc ggcttccgcc agcttgcact gcgccgatcc cgaatagttc
     5821 cgcaaattgg caacgggaac cctccacgta tggaacccca cctgttggat ggctgccaga
     5881 atctcttcct gggtggccac gcccccgagc ggcggatcga ccaggctcac cttttgcgat
     5941 ccgtcatcgg tctggtgaaa ttgcgtctga aactgaagca ccaggtcgcg atgggggtct
     6001 tgtcccccgc ccacaaacgg caccagcttg cgatgcgaat cctttccccg gctcacaaac
     6061 gcaaacgcca cgccttccgt atgcgtcggc gtcgtgtact ggatcaggta aatcggcgct
     6121 ttgccggagt ccgctttttc cggagcggcc agccccagct cgacttcgta cggcttctcg
     6181 ctggcaacat tgagccagtc acgatgatcc gcagccaggc aatggcccgc gtccatcaga
     6241 gtgctcggtg gaggttcggt gatctgcccc cacaaaaaag cactgcagag ggcgagacaa
     6301 catacgacga ggcatctcaa aggaaaggct cctgggtgca gccgccgggc gcaggttttg
     6361 tctactaaga tggaaaaaac acgaaacgcg gagagcaaaa tatggcagag gttgtgacgg
     6421 ttgccagctt tacggagcca ctggaagccg agatggcgaa gctgaggctc gagtcggcgg
     6481 gaattgaagt ctttctctca ggcgaaaatg cccgcatcat ggagccaggc cttggcccgc
     6541 tgcagctcca ggtcagttcc gacgacgagg ccgatgcccg cgccatcctc gccgatcccg
     6601 gcgagccggc cggccaggac tcccaaatat cgtgagacga tatcgcaata cgatatatcg
     6661 tgatacgatg attccatgga aactgcagaa attcatggag ctatctcgct ggcccgtcac
     6721 ggacaaagat ccgatccagg aaaagaacct cagccgctga cccgcgccca gatgcggcgc
     6781 ctggccgagt tccgttttca gttgcggaag tttctccact tcagcagctt ggccgccggc
     6841 gccgccggcg ttcgcaccca gcaataccag ttgctgcagt gcatctgggg tatgccggaa
     6901 ga
//
LOCUS       DASZYN010000598         5036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_406832, whole genome shotgun sequence.
ACCESSION   DASZYN010000598 DASZYN010000000
VERSION     DASZYN010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406832"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="VHE33_15510"
     CDS             <1..1207
                     /locus_tag="VHE33_15510"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HVT98912.1"
                     /translation="HPYARGKEVTAAVAENRPLVRKAAEESLVLLQNKSAMGASPLLP
                     FTSGPHRIALIGPLAGDTQEMVAVWGDNRRLPDVVTLKDALEAREKQAGGSVLYAKGT
                     EISGGSQEGFAEAVDAAKQADVVVMALGESSAMSGEAGSRAHLDLPGNQEQLLEAVAS
                     TGRPMVLLVFSGRPLVLDWAAAHVPAIMEAWFPGVEAGPAIANVLYGDVAPSGKLPMS
                     FPRAVGQEPLYYNRFPTGRPPIDLDLSKPPAGDSRFFSRYIDVPNDALFPFGFGLSYT
                     NFEYSTVRLSRSSLPLREASQPDAHRLITATATVKNSGNRAATEVVQCYVRNLGASLE
                     QPVRSLEGFKRVTLQPGESKEVAFELGFPELSFYNNAGKAVIEPTHYTVWIGGSSLAS
                     DHADFDIVR"
     gene            1277..3244
                     /locus_tag="VHE33_15515"
     CDS             1277..3244
                     /locus_tag="VHE33_15515"
                     /inference="COORDINATES: protein motif:HMM:NF018528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HVT98913.1"
                     /translation="MKCLSIFLRRLDGFAGRRIRVGFVMLAVVASALVAQGESRHIRL
                     SLGPDDVLPTGNEWISLPDIRASDGALTTFNAVSMQNRGLLQVSGEHGGPVLQPIFSA
                     DGKPLPFHNPSWEAIEYWIPEARAAENGIEMTLTWCAPPGSRAAFLRMTATNRRAARA
                     SVSMGVQAFWGRISRVTYTPVELRGERTVGAAPWVNSAEVFSYVTNDTRFAWSLVHPG
                     STAQINMPPTVAVPTVDASKAVELNPGETAEALFVLGVGIEEFSASHNAKALNELLQR
                     NGAETVIEDAAAWCRSRTRTTGQADLDLLMNRNFLFTEFYAWGKTIDTEQFVGVTSRS
                     PRYYVSAAYWDRDAMLWSFPALLDVDPGMAEQALDYALTTQLRNTGTHSRFIDGIVLE
                     DGFQLDEAVAPVIALGAYERKIGDQGFLLEHREAISVLRDRILSRFDPQTGLFSSLQD
                     SQDEFQILPFITYDNALTWRALLDLGELFAKLGDTATADDLRHRAAALHAAILAHCVW
                     SDAPGADGPIFASATDGRKAIFTEIPPGSLMKLATLGFVSEADPVFARTYRWLHSANY
                     QYSYSDKPFGLPGSYRLPFTTSWSVADHLELQAGREQALKILRASNWDGGIITEGVDP
                     TSGKMDQAGRAFATAAGYVAHAICESMCKQK"
     gene            complement(3278..3625)
                     /locus_tag="VHE33_15520"
     CDS             complement(3278..3625)
                     /locus_tag="VHE33_15520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVT98914.1"
                     /translation="MNSLEITVLRHGDVTSLTLRGPLVLGPPADSLRQTLDNLLAHGQ
                     HHVVLEISGIDRLDSSGIGLLVKALQSTKSAGGTVKLVNPPKVVLQTLTMCRLLPLFE
                     VYDTENEAIASFS"
     gene            3921..>5036
                     /locus_tag="VHE33_15525"
     CDS             3921..>5036
                     /locus_tag="VHE33_15525"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HVT98915.1"
                     /translation="MSQPAVVSPLADLSPALLAELELVPRARIVAAAVAEFVPESAVV
                     VFSVDHPQAPVWTARAIAGEITVDSGAIAFEAGTLGALGRERSILQFSAEEVSRDEYA
                     HLDVRQAVTALTYVPMLVGEEVAGAIEIVSFAEAPGEAILEQVAEIANVGAIAFRSAA
                     IYAAERDAGLASVMRLTQLYDIEKVFNATLDMQTLLPVICEKVGDLVHSNGVNLWMVD
                     NEDLLLLQQAGNDPAARPGDRQRGEEGVAGRVGETGEAVLLQAGDAALDARNAKLTDA
                     AVTSLVAAPIMDGESLVGVIEAVRSEGEEPFTDDDFFVLTQVAASAAQALHNSSLLQA
                     ERKIEVLQTLVSVGQEIGSTLNQQRVLQAIVNQPQLVI"
BASE COUNT          957 a         1421 c         1675 g          983 t
ORIGIN      
        1 acatccgtat gcgcgcggca aggaagtgac tgcggcggta gcggagaatc gtccgctggt
       61 tcgaaaagct gccgaggagt cgctcgtgct gctgcaaaac aaatcggcaa tgggtgcgtc
      121 gcccctgctt cctttcacgt ccggaccaca ccggattgct ctgatcggac cactggcggg
      181 cgatacgcag gagatggtgg cggtctgggg cgacaatcgc cgccttccgg atgtagtcac
      241 gctgaaagac gctctggaag cgcgtgagaa gcaggcgggc gggtcggtgc tgtacgcaaa
      301 aggaacagag atctccggtg gttcccagga gggcttcgcc gaggcagtcg atgcggcgaa
      361 gcaagccgat gtggtggtga tggcgttggg tgagtcgagc gccatgagtg gagaagccgg
      421 ctcgcgcgcg catcttgatc tgccgggcaa tcaggagcag ctgctggagg cggtggcgtc
      481 gacgggccgg cccatggtgt tgctggtttt ttcgggccgc ccgctggtgc tggactgggc
      541 tgccgcgcat gtgccggcca tcatggaggc gtggttcccg ggagttgagg ccgggccggc
      601 gatcgcgaat gtgctctatg gcgacgttgc gcccagcgga aagctgccga tgagctttcc
      661 gcgcgccgtt ggccaggagc cgctctacta caatcgattc cccactggac gaccgccgat
      721 cgatcttgac ctttcgaaac cgcctgcggg cgactctcgc ttcttttcgc gctacatcga
      781 tgttccgaac gacgcgctgt ttccttttgg ctttggactg agttacacca actttgagta
      841 ttcgacggtt cggctgtcgc gcagttccct gcccctgcgc gaagccagtc agccggatgc
      901 tcacaggctg atcacggcga cggcaacggt gaaaaattcc ggcaatcgtg cagcgacgga
      961 agtggtgcag tgctacgtgc gcaatctcgg cgcgagtctg gagcaaccgg tgcgcagtct
     1021 tgaggggttc aagcgggtga ctctgcagcc gggtgaatcg aaggaggtag cgttcgagct
     1081 tggatttccg gaattgtcct tctacaacaa cgccggcaaa gcggtgattg agcctacgca
     1141 ttacacggtc tggatcggcg gcagttccct ggccagcgac catgcagatt tcgatatcgt
     1201 gcggtaggga cacggagtgc gcgaccggat gcggagaggc gacggtgtgc gcggcgggca
     1261 gataatggac ccatccatga aatgtctttc gatcttttta cgacggctcg acgggtttgc
     1321 cggccgtcgg atacgtgttg gattcgtgat gctggccgtt gttgcaagcg ctcttgttgc
     1381 tcagggtgaa tcccgccaca tccggctctc tctgggcccg gacgatgttc tgcccaccgg
     1441 caacgagtgg atttctttgc cggacatccg cgcaagcgat ggagcattga cgaccttcaa
     1501 tgctgtctcg atgcaaaacc gcggattact gcaggtgagc ggcgaacacg gcggccccgt
     1561 actgcaaccg atcttcagcg cggatgggaa gcctctgccc ttccacaatc cctcgtggga
     1621 agcgatcgag tattggattc ccgaggctcg cgcagcagag aacggcattg aaatgacgct
     1681 gacctggtgc gcgccgccgg gatcgcgcgc ggcttttctg cgcatgacgg cgaccaaccg
     1741 ccgcgctgcg cgagcgagtg tatcgatggg agttcaggcc ttctggggac gaatcagccg
     1801 tgtcacttat actccggtcg aactgcgcgg cgagcgtacg gtgggagcgg ctccgtgggt
     1861 caattcggca gaggtcttca gctacgtcac caacgacacc cggttcgcgt ggtcgctggt
     1921 tcatcccggc tcgacggcgc agatcaacat gccgccgact gttgccgttc cgacggtgga
     1981 tgccagcaag gctgtcgagc tcaatcccgg ggagacggcg gaggcgctct ttgttctcgg
     2041 cgtcgggatt gaagagttca gcgcatcaca caatgccaaa gcactgaacg agcttctgca
     2101 gcggaatggc gcagagacgg tgattgagga cgctgcagcc tggtgtcgca gccggacgcg
     2161 gacgacgggg caggcggatc tggatctgct gatgaaccgg aattttctat tcacggagtt
     2221 ctacgcgtgg ggaaagacca tcgacaccga gcaattcgtc ggcgtgacgt cacgcagtcc
     2281 gcgctactac gtgtcagccg cttactggga ccgcgatgcg atgctatgga gttttcctgc
     2341 gctgctggat gtcgatccgg ggatggcgga gcaggcgctg gactatgctc ttacgacgca
     2401 gttgcgcaac acgggaacac acagccgttt tatcgacggg attgttcttg aggatggctt
     2461 tcagctcgac gaggccgtgg ctccggtgat cgcgctcggc gcgtatgaac gcaagatcgg
     2521 agaccagggc tttctcctgg agcatcgtga ggcgatatcg gtgctgcgcg atcgcatcct
     2581 gagccgattc gatccgcaga cagggctctt ctcgtcactg caggactcgc aggatgaatt
     2641 tcagatcctg cccttcatca cctacgacaa cgcactcacg tggagagctc ttctcgatct
     2701 cggcgagcta ttcgcgaaac tcggggatac agcaaccgcc gacgatctgc ggcaccgggc
     2761 cgctgcgctg cacgccgcca ttcttgctca ctgtgtgtgg agcgatgcgc cgggcgcaga
     2821 cgggccgatc tttgcctcag cgaccgacgg caggaaagcc atcttcacgg aaataccgcc
     2881 tggatcgctg atgaagctgg ctacgctcgg attcgtgtct gaggccgatc cggttttcgc
     2941 gcgcacgtat cgctggttac attcggcgaa ttaccaatac tcttacagcg acaagccctt
     3001 cggcctgccg gggagttatc gcctgccgtt cacgacatcg tggagcgtgg cggatcatct
     3061 ggagctgcag gcaggtcgcg agcaggcact gaagatcctg cgagcgagca actgggacgg
     3121 cggcatcatc accgaaggcg tcgatccaac gtcaggaaag atggaccagg ccggacgcgc
     3181 cttcgcgacg gcagcgggct acgtggctca cgcgatttgt gagtcgatgt gcaaacagaa
     3241 gtgagtgctg agctgggagg ggcgccgtaa ggccggctca ggaaaacgaa gcaatcgctt
     3301 cgttttctgt gtcgtagacc tcgaagagtg ggagcaggcg acacatggtc agcgtctgca
     3361 gcacaacctt tggcggattg acgagcttca cggtcccgcc ggcgctcttg gtggactgaa
     3421 gggctttcac cagcaaaccg atgccgcttg aatcgagccg atcgatgccg gagatttcga
     3481 gaacgacgtg gtgctggcca tgagcgagaa gattgtccag cgtttgacgc aggctatcgg
     3541 cgggcggacc cagaacgagc ggaccgcgca gcgtcagcga agtgacgtcg ccatgtctga
     3601 gaactgtaat ctccagcgag ttcactccgc cccctgaggc tgacaacggc gcggcgccgg
     3661 gagcaaccgc ggtctttcaa acgggtcatc cgggccgcga tcgctgcttt ccggttgacg
     3721 aaagcgtcag cgaaaaaaat ctccccgcaa aagcaggggc aggataccat accgccaacg
     3781 gtcagacgga aagtgatgcg gcttccccct gaagcttgaa cttccgcagt gcggaaattc
     3841 cccagcagcg cgctctctcc gttcctgtgc gacaaatgaa cgctttcggg cctggagccc
     3901 gaatggaagg agccttgcgg ttgagccagc ctgcagtcgt ttctccatta gcggatctat
     3961 cgccggcact tctggccgag ctggagctgg ttccgcgtgc tcgcatcgtt gccgctgcgg
     4021 tggcagagtt cgttcccgaa tcagctgttg ttgttttctc cgtcgatcat ccgcaggctc
     4081 cggtctggac tgcgcgagcg attgccgggg agatcaccgt cgattcggga gcgatcgcat
     4141 tcgaggcggg aacgctgggc gcgctgggac gcgagcgctc cattctgcag ttcagcgccg
     4201 aagaagtcag ccgtgatgaa tacgctcacc tcgatgttcg ccaggcggtc accgcgttga
     4261 cgtatgtccc gatgctggtg ggggaagaag tcgcgggcgc cattgagatc gtgagcttcg
     4321 ccgaagcgcc aggcgaagcg atcctggagc aggtggcgga gattgcaaac gtcggcgcca
     4381 ttgccttcag aagcgccgct atttacgcag cagaacgcga tgcaggactg gcttcggtga
     4441 tgcggctcac gcaactctac gacatcgaga aggtcttcaa cgccacgctc gatatgcaga
     4501 cgctgctgcc ggtgatctgc gaaaaggtcg gcgatctggt ccacagcaac ggggtgaacc
     4561 tgtggatggt cgacaacgag gacctgctgt tactgcaaca ggcgggtaac gatccggctg
     4621 cgcgtcccgg ggatcggcag cgcggcgagg agggagtcgc gggccgcgtg ggtgaaaccg
     4681 gcgaagcggt cctgctgcag gcgggcgatg cagcgctgga cgctcgcaat gcgaaattga
     4741 cagatgcggc ggtgacgtcg ctcgtggccg cgcccatcat ggatggggaa agcctggttg
     4801 gggtgattga ggcggttcgc agcgagggcg aggagccttt taccgacgac gatttctttg
     4861 tgctgaccca ggttgcggcc tcggcagcgc aggcgctgca caactcgtcg ctgctgcagg
     4921 cagaacgaaa aatcgaagtg ctgcagacgc tggtgagcgt ggggcaagag atcggttcga
     4981 cactgaacca gcagcgtgtc ctgcaggcga tcgtgaatca gccgcagctg gtgatt
//
LOCUS       DASZYN010000599         2995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_91631, whole genome shotgun sequence.
ACCESSION   DASZYN010000599 DASZYN010000000
VERSION     DASZYN010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91631"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1576)
                     /locus_tag="VHE33_15530"
     CDS             complement(<1..1576)
                     /locus_tag="VHE33_15530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HVT98916.1"
                     /translation="MADSGSGQQAQNFGINPWLIAISVMLATFMEVLDTAIASVALPY
                     IAGSLSASNDEATWVLTSYLVANAVILPASNWFSLRFGRKRFLITCVIIFTIASFFCG
                     AAPTLGFILIARVIQGAGGGALQPLSQSILLESFPPEKRGAAMAFFAFGVVVAPVLGP
                     TLGGWLTDTYSWRYAFYINIPVGILAVYMISRYVHDPEYIRKAKPSKFDTIGIGTLAV
                     WTGCLQIILDKGQEDDWFGSIWIRWAALFLIAALAWFIIHSWRSKTPLVNLRTLKDRN
                     FGLGCGLMLMFGLCIYSMVTVLPLFYQELLGYTAFAAGLVVGPRGIGSIIGMPVIGYL
                     GNKVDPRYLLTFGFVGFGVMSLVFGNLTLQISPWTLLVPILVTGFALSFVFVPITTQA
                     YSTLRNDQIGNASGIFNLVRNIGGSIGISIAQTLLVRRSSAHQSFITGYVSQTQYWFE
                     QRLSALTGYLTHHTAAPNAAGAAADTLYRQLGRQALLWAYVDVFRWMSLICFVCVVLV
                     WFFKKARPGARPPAGAH"
     gene            1883..>2995
                     /locus_tag="VHE33_15535"
     CDS             1883..>2995
                     /locus_tag="VHE33_15535"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HVT98917.1"
                     /translation="MAKIIGIDLGTTNSVVAVMEGGEPKVIANEEGGRTTPSVVAFTK
                     SGERLVGQVAKRQAITNPANTVYSIKRFMGRRFNEVNDEMKMVPYKVVQQGDHVAVEA
                     QGKLYTPPEISALILQKLKKAAEDHLGTTVTEAVITVPAYFNDAQRQATKDAGKIAGL
                     DVKRIVNEPTAAALAYGLDKKKDETIAVYDFGGGTFDISILEVGEGVIEVKSTNGDTH
                     LGGDNIDQRIVDWLIEEFKKEEGLDLRAKGNEMALQRLRDSAERAKIELSTALETEIN
                     LPFITADASGPKHLVKKLTRAKLEQLVEEIIQRSVEPCKKALADAGVDASKIDEVVLV
                     GGQTRMPRIQALVKELFGKEGHKGVNPDEVVAVGAAV"
BASE COUNT          665 a          810 c         1037 g          483 t
ORIGIN      
        1 agtgcgcgcc ggccggcggt cgcgcgcccg ggcgggcttt cttgaagaac caaaccagca
       61 caacgcagac gaagcagatc aggctcatcc agcggaagac gtcgacgtag gcccacagca
      121 gggcctggcg gcccagctgg cggtaaagag tgtcggccgc ggctcccgcg gcattgggtg
      181 cagcggtgtg atgcgtgaga taaccggtga gcgcgctgag acgctgctcg aaccagtact
      241 gcgtttgtga gacgtagccg gtaatgaagg actggtgggc gctgctgcgc cggacgagca
      301 gcgtctgggc gatggagatg ccgatcgaac cgccaatgtt gcggacgagg ttgaagatgc
      361 cgctggcgtt tccgatctgg tcgttgcgca gcgtgctgta cgcctgggtc gtgatgggca
      421 cgaagacaaa gctcagggca aacccggtaa cgaggatggg gaccagcagg gtccacgggc
      481 tgatctggag ggtgagattg ccgaacacca gactcatgac accgaagccg acgaagccga
      541 aggtgagcag gtagcgggga tcgactttgt tgcccaaata gccgatgacc ggcataccaa
      601 taatggagcc gatgccacgg gggccgacaa cgagcccggc ggcgaaggcg gtgtagccca
      661 gcagttcctg atagaagagc ggcagcaccg tcaccatcga gtagatacac aggccaaaca
      721 tcagcatcag gccgcagccg aggccgaagt tgcggtcttt gagcgtgcgc aggttcacca
      781 gcggagtctt gctccgccag gaatgaatga tgaaccaggc gagtgccgcg atgaggaaga
      841 gggcggccca gcgaatccag atcgagccga accagtcgtc ttcctggcct ttgtcgagga
      901 tgatctggag gcatccggtc cagaccgcga gtgtgccaat gccgatggtg tcgaacttgc
      961 tgggtttggc tttgcgaatg tactcgggat cgtgaacgta gcggctgatc atgtagaccg
     1021 cgaggatccc gaccgggatg ttgatgtaga aggcgtagcg ccagctgtag gtgtcggtaa
     1081 gccagccgcc cagagtcggg ccgaggacgg gagcaaccac cacaccgaag gcgaagaagg
     1141 ccatggcggc gccgcgcttc tccggcggga agctctcgag caggatcgac tgggagaggg
     1201 gctggagggc tccgccgccg gcgccctgga tgacgcgcgc gataaggatg aagccgagag
     1261 tgggcgccgc tccgcagaag aacgaggcaa tggtgaagat gatgacgcag gtgatgagga
     1321 agcgcttgcg tccgaagcgc agggagaacc agttgctggc gggcagaatg acggcgttag
     1381 cgaccagata gctggtcagg acccaggtgg cctcgtcgtt cgaggccgag agagagcccg
     1441 caatataagg cagcgcgacc gaggcgatgg cggtgtcgag cacctccatg aaggtggcca
     1501 gcatcacgct gatcgcaatc agccatggat tgatgccaaa attctgcgcc tgctggccgc
     1561 tgccgctatc cgccatgttc ctgcctggat tgttggagtt acgtgggtaa aagctggccg
     1621 gggaatgccg actatgatgc ctcagcgggg ccaggaaacg tatcttcttt tctgcgtttt
     1681 agatacggcg ggggtgggga cggatgcagg cgagctctgg aaaacgttcc tgccggcgca
     1741 gaatggggca aaaacaccaa tcttttgaat aacttccgtt tctgaatcgc gaattccatt
     1801 tctcgcatca gatagattga caataagtga ctcaagtgcg atgctgacca ggcagggcac
     1861 tcattcagga gcagcaacaa caatggcaaa gattatcggt attgacctcg gcacgaccaa
     1921 ctcggtcgtg gccgtgatgg agggcggtga gcccaaagtg atcgccaacg aagagggtgg
     1981 ccgcaccacg ccttccgtcg tcgcgttcac gaagtcggga gagcgcctcg taggccaggt
     2041 ggccaagcgc caggcgatca ccaaccccgc gaacacggtt tactcgatca agcggttcat
     2101 ggggcgtcgc ttcaacgaag tcaacgacga gatgaagatg gtgccctaca aggtcgtcca
     2161 gcagggcgat cacgtggccg tggaggcgca gggcaagctg tacacgccgc cggagatttc
     2221 ggcgctcatc ctgcagaagc tgaagaaggc tgcggaagac catctgggaa cgaccgtcac
     2281 cgaggccgtg atcacggtgc cggcctactt taatgatgcg cagcgccagg cgaccaagga
     2341 cgcaggcaag attgccgggc tcgacgtgaa gcgcatcgtg aacgagccga cggcggccgc
     2401 gttggcctac ggcctcgaca aaaagaagga cgagacgatc gccgtgtacg actttggcgg
     2461 cggcaccttc gatatttcga tcctcgaagt cggcgaaggc gtcattgagg tgaagtcgac
     2521 caacggcgac acccacctgg gcggcgacaa catcgaccag cggatcgtgg actggctcat
     2581 tgaggagttc aagaaggaag agggtctgga cctgcgcgcc aagggcaacg agatggcgct
     2641 gcagcggctc cgcgattcgg ccgagcgcgc caagatcgag ctgtcgacgg cgctcgagac
     2701 cgagatcaac ctgccgttta tcaccgccga tgcgtctgga ccgaagcacc tcgtcaagaa
     2761 gctgacgcgg gcgaagctcg agcaactggt ggaggagatc atccagcgct cggtggagcc
     2821 gtgcaagaag gctttggccg atgccggcgt ggatgcaagc aagatcgacg aggtcgtgct
     2881 cgtcggcgga cagacgcgca tgccgcggat ccaggcgctg gtgaaggagc tgttcggcaa
     2941 ggaaggccac aagggtgtga accccgacga agtggtggcc gtgggcgcgg cggtc
//
LOCUS       DASZYN010000600         4646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_607565, whole genome shotgun sequence.
ACCESSION   DASZYN010000600 DASZYN010000000
VERSION     DASZYN010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607565"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VHE33_15540"
     CDS             complement(<1..942)
                     /locus_tag="VHE33_15540"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98918.1"
                     /translation="MRFRLHPALGLLFTVAFTAHAQAASNPIKITVDLTDAPRRILHA
                     HMTIPVSPGPLTLLYAKWIPGEHMPDGPIDNLAGLFITANGQQLPWTRDDVNMYAIRV
                     TVPDGVTQLNVKDDFLATAAASGFSAGASTSANLALLSWNELVLYPAGHKDDADIEVQ
                     PSVVLPEGWQYGTALTRTGGDATHVDFANVSLEQLIDSPLLTGRYFVEIALAPEVTPK
                     HFLDIAADGPEDLKVSPGAVQAYSNLVRETGALYQSRHYNSYHFLVTASDQVAHFGLE
                     HHQSSDDRVAERTFIDDNLQLLSADLLPHEFTHSWNGK"
     gene            988..1488
                     /locus_tag="VHE33_15545"
     CDS             988..1488
                     /locus_tag="VHE33_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98919.1"
                     /translation="MRAAKSLDLLQGRRTLARPEVRVMLDGTLHWNFGLAWIAFALGV
                     ALHVVDEARHDFLATYNPNAMAIRRRLHIPFPPVFTFRAWLGGLIAGVCVLLLLSPFA
                     LHGVHWIRIVALPLAILIGIGNGLGHIGGSIYYRRWMAGILSSPVLLAVGTWLLWSAW
                     AEFQHG"
     gene            1539..2228
                     /gene="tmk"
                     /locus_tag="VHE33_15550"
     CDS             1539..2228
                     /gene="tmk"
                     /locus_tag="VHE33_15550"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HVT98920.1"
                     /translation="MASGFFLTFEGLDGSGKSTQLRKLAEHLQAQGLAITVTRQPGGT
                     HLGDRVRGLILDSKTERIDPRTELGLMFSDRAQCIAEVIAPALHGGNVVLCDRFTDST
                     EAYQGGGRQLGSEIVLRLHETMCGGLQPDLTLLLLADFDRSLARARRRNTRNAAAGQD
                     EGRFEREDLNFYRRVYDKYREIAEREPRRVAIIDGDRSIEVIHHEIRELVDDRLRAAG
                     MLPVQPAAGVH"
     gene            2359..3624
                     /locus_tag="VHE33_15555"
     CDS             2359..3624
                     /locus_tag="VHE33_15555"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HVT98921.1"
                     /translation="MDISRPDIRKKKLRRQIIVLAIVAVGVTAAAWAVMRLKPAVPTV
                     DASTIWPDTVKRGDMIRNVRGLGTLVPREDSIELIPTQTEATVVRIRVLPGARVTPDT
                     VLMDLADPQLQQQLLNAQLAVKQAEADYKSLQATLQSTLMDKKSTGAQINSQYSQAQL
                     QANTDQQLYKLGVISGIQYNQSKNNAEQLTTQHQISQEQLDMNQKAIEVQLASAGTKV
                     DQAKAQLALYQSQEDALHVKAGISGVVASLATPVSVGEHVAAGTSVAEVIDPTKLKAA
                     LQIAETQAHDILLGQKAEIDTHNGIVPGHVTRIDPSVLNGTRTVDVTLDGELPAGAVP
                     QLSVDGTIELAHLHDVLYVGRPAFGNENSTISLFRMDANGKTATRVQVQVGKASTTEI
                     QIMSGLNDGDRVILSDMSRYDNTDKVRLE"
     gene            3681..4400
                     /locus_tag="VHE33_15560"
     CDS             3681..4400
                     /locus_tag="VHE33_15560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT98922.1"
                     /translation="MDNNQALIEIEGLTKVFYTDEIETHALSGVHMKIDKGEYVAISG
                     PSGCGKSTLLSIIGLLDTPTGGSYTLNSHSVENLNFSQRSRIRNQEIGFIFQSFNLIG
                     DLTVYENVELPLTYRSGMSSAERKRRVQESLERVSMAHRIRHYPSQLSGGQQQRVAVA
                     RALAGSPSILLADEPTGNLDSRNAEAVMELMQNLHREGATICMVTHDPRFARHADRQI
                     HLFDGKVVAEGESLEQVLEVK"
BASE COUNT          917 a         1428 c         1408 g          893 t
ORIGIN      
        1 ttttccgttc cacgagtgcg tgaactcgtg cggcaggaga tcggcggaga gcagttgcag
       61 gttgtcgtcg atgaaggtgc gctccgccac gcgatcgtcg cttgactgat gatgctcgag
      121 gccgaagtgc gccacctgat cgcttgccgt gaccaggaag tgatagctgt tgtagtgccg
      181 tgactgatag agagccccgg tttcgcgcac gagattgctg tatgcctgca cggcgccggg
      241 agaaaccttc agatcttccg ggccatcggc ggcgatatcc agaaaatgct tcggtgtcac
      301 ctccggcgcg agcgcgattt cgacgaaata cctgcccgtc agcagcggcg agtcgatcag
      361 ctgttccagg gaaacgttgg cgaaatcaac atgggtggca tcgccgccag ttctggtcag
      421 cgccgttccg tactgccatc cttcgggcag aaccaccgat ggctggacct cgatatccgc
      481 gtcgtccttg tgcccggcgg gatagagcac cagctcattc cagctcagca gcgccagatt
      541 tgcgcttgtg gatgcacccg ctgaaaagcc gctggccgcg gccgtggcca ggaagtcgtc
      601 tttgacgttg agctgggtta ctccgtcagg caccgtcacg cggatcgcgt acatgtttac
      661 gtcgtcgcgc gtccatggaa gctgctgtcc gttggcggtg atgaagagtc ctgccaggtt
      721 gtcgatcggc ccatccggca tgtgctcgcc ggggatccac tttgcgtaga gcagcgtgag
      781 cggtcctgga ctcaccggaa tggtcatgtg cgcatgcaga attctgcgcg gcgcgtcggt
      841 caggtcaacg gtgattttta tgggattcga agcggcttgg gcgtgcgcgg taaaggccac
      901 agtgaaaagc aaccccaggg cggggtgcag gcgaaagcgc atagtcctcc tgaccaaagt
      961 gcgaagaccc agtgtactga gggcggcgtg cgcgccgcga aatcgcttga ccttctgcag
     1021 gggcggcgta cactggcgcg tccggaggta cgtgtcatgc tcgacggcac gctgcactgg
     1081 aacttcgggc tagcctggat tgcgtttgcc ctcggggtgg cgctgcacgt cgtcgatgag
     1141 gcgcgccacg actttctcgc cacttacaat cccaacgcga tggccatccg ccgtcgtctg
     1201 catattccct ttccgccggt gtttacgttt cgagcgtggc tcggcgggtt gattgccgga
     1261 gtgtgcgttc ttctgttgct ctcgccgttt gcgctgcacg gtgtgcattg gatccggatc
     1321 gtggctctgc cactcgcgat cctcatcggg atcggcaacg gcctgggaca catcggcggc
     1381 tcgatctact atcgccgctg gatggccggc atcctgagtt cgccggtctt gctggccgtc
     1441 gggacctggc tgctttggag tgcgtgggcg gagttccagc acggttagca ttggcgccgg
     1501 tactgtaccc tcctcccagc accctgtacc ctgcttttat ggcgagtggc ttcttcctga
     1561 cttttgaagg cctcgacggc tccggcaagt cgacgcagtt acgcaagctc gccgagcatc
     1621 ttcaggccca gggcctggcg attaccgtca cgcgccagcc gggaggcacc catcttggcg
     1681 accgggtccg tggtctgatc ctcgattcca aaactgagcg gatcgatccg aggaccgaac
     1741 tcggcctgat gttctccgac cgcgcgcaat gcatcgccga ggtgatcgcg ccggcactgc
     1801 atggcggcaa tgtggtcctc tgcgaccgct tcacggattc gacggaggct taccagggcg
     1861 ggggacggca actcggcagc gagattgtcc tgcggcttca cgaaaccatg tgcggggggc
     1921 tgcagccgga cctcactctc ctgctcctcg ccgacttcga tcgatccctg gcccgcgctc
     1981 gccggcgcaa tacccgcaac gccgcagccg gtcaggacga aggccggttt gagcgcgagg
     2041 atctgaactt ttaccgccgc gtgtatgaca agtaccgcga aatcgccgag cgggagccca
     2101 ggcgtgtggc aatcatcgat ggggatcgct ccatcgaggt catccatcac gaaatcaggg
     2161 agttggtcga cgacaggctg cgggcggccg ggatgctccc tgtccagcct gccgccggtg
     2221 tccactagtg gacaccgggt tccgcattcg atcactcgac gcctctcggt ctctgcctga
     2281 acgaagctca aataaaacat tgcacgagac ttcggaccat ctcccaaatg gcgcggcaca
     2341 tgctattccc tttatgtaat ggatatctca cggccagaca tcaggaagaa aaagctccgc
     2401 cggcagatca ttgtgctggc tattgttgca gttggcgtca ccgcggccgc ctgggctgtt
     2461 atgcgcctca aacccgctgt cccgacggtt gacgccagta cgatctggcc cgacacggtg
     2521 aagcgcggcg acatgatccg caacgtccgt ggccttggca cgctggtccc ccgcgaggac
     2581 agcatcgagc tcatcccgac ccagacggag gcgacggtgg tgcgcattcg ggtcctgccg
     2641 ggcgcgcggg tgactcccga tacggtcctg atggatctcg ccgatccgca acttcagcag
     2701 cagttgctga acgcacagct cgccgtcaag caggcagaag ccgattacaa gagcctgcag
     2761 gcaacgctgc aaagcacgct gatggacaag aagtccaccg gggcgcagat caactcacaa
     2821 tatagtcagg cgcagcttca ggccaacacc gaccagcagc tctacaagct gggggtgatc
     2881 agcggcatcc agtacaacca gtcaaagaac aacgcggagc agctgaccac acaacaccag
     2941 atcagccagg aacagctcga tatgaaccag aaggcgatcg aagtccagct cgcctccgcg
     3001 gggacaaaag tcgaccaggc caaggcgcaa ctggctctgt accagagcca ggaggacgcc
     3061 ctgcatgtga aagccggcat ttcgggcgtg gtcgcctccc tggccactcc tgtttctgtg
     3121 ggcgaacacg ttgcagccgg aacgtcggtg gctgaggtga tcgatccgac gaagctgaag
     3181 gctgccctgc aaatcgccga gacgcaggcg cacgatattc tgctcggcca gaaagccgag
     3241 atcgacacgc ataacggcat tgttccggga cacgttaccc gaattgatcc ctccgtattg
     3301 aacggaacgc gtaccgttga cgtaacactc gatggggagc ttccagccgg agcagttccg
     3361 cagctgagcg tggacggaac catcgaactc gcacacctgc acgatgtgct ctatgttggg
     3421 cggcccgcat tcggcaatga gaacagcacg atcagcctgt tccgcatgga cgccaacggc
     3481 aaaaccgcta cccgggtcca ggtgcaggtg ggtaaggctt cgacgacgga gattcagatt
     3541 atgagtggcc tgaacgatgg ggatcgtgtc atcctttcgg acatgtcacg ctacgacaac
     3601 acagataaag ttcggctgga gtaggcactt cccccaggaa gcaacggcag cgcaaacttc
     3661 ttcaggagca ggagagcagg atggacaaca atcaggcgtt gatcgaaatt gaaggcctca
     3721 ccaaggtctt ctacaccgac gagatcgaaa cccacgcgct gtccggcgtc cacatgaaga
     3781 tcgacaaggg tgagtatgtc gccatctccg gtccttccgg atgcggcaag tccacgctcc
     3841 tgtccatcat tggcctgctc gacacgccca ctggcgggag ctatacgctc aacagccatt
     3901 cggtcgagaa tctgaacttc tcgcagcgtt cgcgcatccg caaccaggag atcggcttca
     3961 tcttccagag cttcaatctc atcggcgatc tcaccgtgta tgaaaacgtt gagctgccgc
     4021 tgacctatcg ctccggaatg tcgtcggctg agcgcaaacg ccgcgtgcag gagtcgctcg
     4081 agcgcgtgag catggcacac cgtatccgcc actatccctc gcagctctcc ggcggtcagc
     4141 agcagcgtgt tgctgtggcc cgcgccctgg ccggttcgcc ctcgatcctg ctggccgacg
     4201 agcccaccgg aaacctggac tcgcgcaacg ccgaggccgt gatggagttg atgcagaacc
     4261 tccatcgcga aggcgcaacc atctgcatgg tgacgcacga tccacgcttc gcgcgccatg
     4321 ccgatcgcca gattcacctc ttcgacggca aggtagtcgc ggaaggcgag tcgctggagc
     4381 aggttctcga agtcaagtaa tcggctgcac gagtcccgcc gctcccatct acgggactcg
     4441 tgtgtccagg cgtctgattc cggcctgtac gttaacaaga aggaaccatg attcagctca
     4501 aaaacctcga acgcagttac aagaccggcg ccggccaaac ctgggtcctg cgccgcgtca
     4561 accttgaaat taaagagggc gagttcctta cgatcatggg cccgtccgga gcgggaaagt
     4621 cctcgcttct gaacgtcctc gccctg
//
LOCUS       DASZYN010000601         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1055862, whole genome shotgun sequence.
ACCESSION   DASZYN010000601 DASZYN010000000
VERSION     DASZYN010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055862"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            675..1871
                     /gene="ltrA"
                     /locus_tag="VHE33_15565"
     CDS             675..1871
                     /gene="ltrA"
                     /locus_tag="VHE33_15565"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HVT98923.1"
                     /translation="MRRDILEHALQRIAANGGAPGADGQTIESIRSSPEKQSQWLDSL
                     QRELKTRTYRPLPVRRVWIPKANGGQRPLGIPTVKDRVVQMAAMLVLLPIFEADFHPR
                     SYGFRPRRNAHQAIEEIVRALRHGHAEVLDADLSKYFDTIPHRGLLKLLARRISDGSV
                     LWLIKIWLRAPVQEEDKDGTKRIKPNSSGTPQGGVISPLLANLYLNALDWAVNEKVEG
                     KPVLVRYADDFVILARPGQGENLRQRLRQWLAAQGLTLNEEKTRLVNRQDGFNFLGFS
                     VRWQRGRNTGRWYAHVQPSAKSEQRLRDNVRGILSHWTLHKHITEAVEELNPLLRGWH
                     GYFHFRNSTQVIWGLQGWVRARFKGWLWRKHRRRKGKWKGYPDHLLHGRYGLWLMPTR
                     APWKPT"
BASE COUNT          480 a          571 c          635 g          325 t
ORIGIN      
        1 ccgcagcagc ccgtgaaggt ggttgcgctc gtgggtgcaa gtcccacccg tcgaattgga
       61 cggttcacga cggaacggcc cggtgcctgt cggccccgca aggggaagag gggagtaagc
      121 agtccgggta tagaaccgat gggcctgaat gaagatgaac cacagctaat gagcctcgaa
      181 ataatactga accggagtcg ccgagtcgct ggtgtgatga tgaaggccga cgtctgcgtg
      241 gcggacaacc gtcagcccgc gcagtctccg gcggggtcgg cgtggcaggc acgtcgggaa
      301 gggcaaccac agataaagcg gggagagcgg ggccgatgag caaggagcaa tccaaggctc
      361 aatctgacgg gcaagccggt tcgtccggca agacggcagg cgtcggttcc gcagtcggag
      421 gagtccatag taagcgagcc cacagatggc acgatgagtc ggggaacatt accccggaac
      481 atctggcctg gctcaaggct cggagaaggg actctgcttg tacgcaagcg tggcagagga
      541 gcgaaggaca aggggatggt tccgcgcgag cgggaataat aactcctgag aagattcgca
      601 agctccaacg cacgctctat cgcaaagcca aagccgaacc gaaatatcgg ttctggagtc
      661 tctacggaga catcatgcgc cgggacattt tggagcacgc gctccaaaga atcgcggcaa
      721 acgggggcgc tccaggtgcg gatggccaaa ccatcgaaag catcaggagc agcccggaaa
      781 agcagagcca atggctagac tcgttgcaac gggaactcaa gaccaggact taccggcccc
      841 tgccggttcg acgggtctgg atacccaaag ccaacggggg ccagcggcct ctgggcatac
      901 cgaccgtcaa agaccgggtg gtacagatgg cggcgatgct ggtgctcttg ccgatatttg
      961 aagcggactt tcatccgcgc agctacggct ttcggccccg gcgcaacgcc catcaagcca
     1021 tcgaagaaat cgttcgggcg ctacgccatg gccatgccga agtgctggat gccgacttgt
     1081 cgaaatactt cgacaccatc ccgcaccgag gcttgctcaa gctgttggcc cggcgaatca
     1141 gtgatggcag cgtcctgtgg ctcattaaaa tatggctgcg ggcacctgtg caggaagagg
     1201 acaaggacgg caccaagcga atcaaaccta actccagcgg cacaccgcag ggcggagtga
     1261 tctcgccgtt gctggccaac ctctacctca acgccttgga ctgggcggtc aacgaaaagg
     1321 tcgaaggcaa accggtcctg gtccgctatg cggatgactt cgtcatcctt gccagaccgg
     1381 gacaagggga aaacctgcga caaaggctgc gccagtggct ggcggcgcaa ggactgacgc
     1441 tcaacgaaga aaagacccgg ctggtcaatc gccaggacgg gttcaacttc ctgggcttca
     1501 gcgtgcgctg gcaacgcggc cgcaatacgg gccgatggta tgcgcacgtt caaccctccg
     1561 ccaaaagcga gcagcgactg cgtgacaatg tgcgcggcat acttagccat tggacattgc
     1621 acaaacacat caccgaggcg gtagaggaac tcaacccttt actgcgcggc tggcacggat
     1681 acttccactt ccgaaacagt acgcaggtga tctggggcct gcaagggtgg gtccgtgcac
     1741 ggttcaaagg atggctgtgg cgtaaacatc gtcgccgcaa aggcaaatgg aagggctacc
     1801 ccgaccacct gctacatggc cgctatggcc tgtggctgat gccgacgcga gcaccttgga
     1861 aaccaacatg actgaatgca atgcgatgaa gcgtcccgga aagccggata cgggaaatcc
     1921 gttcgtccgg tttgatgagg ggcgaagtgg caacgcagga ctgacaacta cggtcggttc
     1981 aacccgcctg ttaccacttc gcctactcta c
//
LOCUS       DASZYN010000602         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_934958, whole genome shotgun sequence.
ACCESSION   DASZYN010000602 DASZYN010000000
VERSION     DASZYN010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934958"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..1556
                     /gene="narH"
                     /locus_tag="VHE33_15570"
     CDS             15..1556
                     /gene="narH"
                     /locus_tag="VHE33_15570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HVT98924.1"
                     /translation="MDIRAQVSMVFHLDKCIGCHTCSIACKNIWTDRKGTEYMYWNNV
                     ETKPGTGYPTRWEDQTKYRGGWVVDGQRQKSLRLRLQGKWGTLSNIFYNPYLPTLDDY
                     FEPWTYDYQNLINAPLADEQPTARAISMVTGKYMDTIEAGPNWDDDLGGSQVYANNDP
                     NLDGASEEEMRQINEINSTVFFYLPRICNHCLNPGCVAACPQGALYKRGEDGVVLVSQ
                     ERCRAWRMCISGCPYKKTYFNWSTGKAEKCILCYPRLESGQPPACFHSCVGRIRYIGL
                     VLYDADAIEETAKSPQDQLVMAQRNIIKDPFDPEIIAAARANGIPDAKIEAAQKSPVY
                     QFVKKWGIALPLHPEFRTLPMLFYVPPLGPVLAKVENGVYDNIASESRLGPLMSSLER
                     SRVSLRYMASLLAGGNEEIIKGVYKKLVAVRVYMRSKKVKDIPDEEVQRALAEGQTTA
                     AEVEAIWRLTSMPTFEERFVVPPMERETAVDALFPQLDPVSHNYPIRKGAVGAGFHTD
                     PARGP"
BASE COUNT          324 a          483 c          526 g          265 t
ORIGIN      
        1 gaaggagtat agacatggac attcgcgctc aagtttcgat ggtctttcac ctggataagt
       61 gcatcggctg tcacacctgc agcatcgcgt gcaagaacat ctggaccgat cgcaagggca
      121 ccgagtacat gtactggaac aacgtggaga cgaagccggg caccggctat ccgacgcgtt
      181 gggaagacca gaccaaatac cgtggcggct gggtcgtcga cgggcagcgg cagaagagcc
      241 tgcggctgcg gctgcagggc aagtggggca cgctcagcaa catcttctac aacccctacc
      301 tgccgacgct ggacgactac ttcgagccct ggacctacga ttaccagaac ctgatcaacg
      361 cgcccttggc cgacgagcag ccgaccgcgc gcgcgatctc gatggtgacg ggcaagtaca
      421 tggacacgat cgaggcgggc ccgaactggg acgacgacct cggcggttcg caggtctacg
      481 cgaacaacga cccgaacctc gacggcgcct ccgaggagga gatgcgccag atcaacgaga
      541 tcaacagcac ggtgttcttc tatctgccgc gcatctgcaa tcactgcctc aaccccggct
      601 gcgttgcggc gtgtccgcag ggcgcgctct acaagcgcgg cgaggacggc gtggtgctgg
      661 tgagccagga gcgctgccgc gcctggcgca tgtgcatctc gggatgtccg tacaagaaga
      721 cctacttcaa ctggtcgacc ggcaaggccg agaaatgcat cctgtgctat ccgcgccttg
      781 agagcggcca gccgccggcc tgcttccact cctgcgtggg ccgcatccgg tacatcggcc
      841 tggtgcttta cgatgcggat gcaatcgagg agacggcgaa gtcgccgcag gatcagctgg
      901 tgatggcgca gcgcaacatc atcaaggatc cgttcgatcc ggagatcatc gcagccgcga
      961 gagccaacgg cattcccgat gcgaagatcg aagcggcgca gaagtcgccg gtctaccagt
     1021 tcgtcaagaa gtggggcatc gcgctgccgc tgcatccgga gttccgcacg ctgccgatgc
     1081 tgttctacgt gccgccgctg ggaccggttc tcgccaaggt ggagaacggc gtgtacgaca
     1141 acatcgcctc cgaatcgaga ctgggtccgt tgatgagttc gctggagcgg tcacgcgtct
     1201 cgctgcgcta catggcgagc ctgctggcgg gcggcaacga ggagatcatc aagggagtct
     1261 acaagaagct ggtcgcggtg cgcgtgtaca tgcggtcgaa gaaggtgaag gatatcccgg
     1321 acgaggaggt gcagcgcgcg cttgccgaag gccagaccac ggcggccgag gtcgaggcga
     1381 tctggcggct gacgtcgatg ccgaccttcg aggagcggtt cgtggttccg ccgatggagc
     1441 gtgagacggc ggtcgatgcg ttgttcccgc agctcgatcc ggtctcgcac aactacccga
     1501 tccgcaaggg cgcggtcggt gccggcttcc acaccgaccc ggcgagagga ccataggctc
     1561 tctccaggtt acgcaacaca aagacagcgg ttgggctt
//
LOCUS       DASZYN010000603         4827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_860044, whole genome shotgun sequence.
ACCESSION   DASZYN010000603 DASZYN010000000
VERSION     DASZYN010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860044"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VHE33_15575"
     CDS             <1..209
                     /locus_tag="VHE33_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98925.1"
                     /translation="ILNSGSVTVSAEEARLFERVLSKGLAPGLLVRVLREIFPLLGAG
                     APVVASGFAEDDEGWRVQPARERG"
     gene            212..1162
                     /locus_tag="VHE33_15580"
     CDS             212..1162
                     /locus_tag="VHE33_15580"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVT98926.1"
                     /translation="MSSGSIQRWCDPRVILLATNLHDPPDLMRHAVAEARRSGARLLL
                     VHVIPSLAPQEDAAGSPPRVFLFPSKDCAANALEQLALQLQWQGVLCEAVVLRGRPAE
                     QICALVPLRKVDRVFVASRGRAAGASAGPCVAEELVSALAIPVCVVSRQADPAGMSGA
                     RASRVLLALSLRSMRREYVDFAFGVARARGARLTLLHVLDGSSIRERHRASAHDRAML
                     RLAALAATCDARPRGTDLVVRDGDPAAQIVEQAGCPFQDLIVLGSSSLRSSSAMLGGN
                     ILRRVIAEARCPVITLKPPIAEFRAEPDPEWPILRTGSFD"
     gene            1194..1526
                     /locus_tag="VHE33_15585"
     CDS             1194..1526
                     /locus_tag="VHE33_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98927.1"
                     /translation="MKCANPLCEHRLHYLRGGTLRLLEFENAPEHRLRGGGGGFPVIQ
                     HSARYFWLCSECSGILILRRWTQCGLVLQSRRQPEEASPLTWTVPAEPAVDVENRGSF
                     RIPRVRTA"
     gene            1749..2309
                     /locus_tag="VHE33_15590"
     CDS             1749..2309
                     /locus_tag="VHE33_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98928.1"
                     /translation="MSLTKFRKIAYTTFVATLLLALAPLLRAESVDDSREITQLLSQA
                     KATATLVTHDAETLESYTRSRISWQTHARQLERMRGHTNELIEDVRELSALRSQGSPW
                     QQEAIDRINPMLKDMADHLTATIEHLNDNRSSIHFQPYQDYARGNRIFADKTLVAIRD
                     FVDYGEARAAADSLEQKLTLPAASGE"
     gene            2401..3294
                     /locus_tag="VHE33_15595"
     CDS             2401..3294
                     /locus_tag="VHE33_15595"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HVT98929.1"
                     /translation="MGPSSIIESESLAVPIQAFPSRANHPHTADRLRVVTYNVHKCRG
                     LDGRVLPHRILRVLEEIDADVIALQEVVCREEKSAERHQARYLAERLGLFCELGENRR
                     HRGGAYGNVLLSRYPILHARNHDISVPGRERRGCLHVELQPENSAVLHIFNLHLGTAL
                     YERRMQARQMFGERLFPGAELSGNKIVLGDFNEWTRGLPSRLFNLHFESAHSYMDAHR
                     TRTFPGVLPMLPLDHIYFDRELQLDHVRIHRTPAALMASDHLPVVAEFTLPAESGNAA
                     VSEQGAALLPPSTDRLPEARV"
     gene            3473..>4827
                     /locus_tag="VHE33_15600"
     CDS             3473..>4827
                     /locus_tag="VHE33_15600"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HVT98930.1"
                     /translation="MPFCYRASTRAPYWVGFTLSWICLCTPLPASAQNGAPYAAPVAN
                     AGAVRPSNAQLASPEIERKVDALVRQMTLEEKLGQLVQYGDTGDSANAQLANDNQAPG
                     KNPETHIKIDPMELAATGRLGSMLNVVGAERTNTFQRAAVEKSRLHIPLLFGADILHG
                     YRTIYPIPLGLAATFDPDLVSSVSHVSAQEATTAGVRWFYSPMVDISRDPRWGRTQEG
                     AGEDAYLGAAMARAYIHGYQGEDLSRADSVAASVKHFAAYGAAEAGREYNTTDMSLSR
                     LQQDYLPPYKAAVKAGAATIMSAFNALNGVPSTANPYLLHDVLRRNWGFDGFVVSDYT
                     SVMELRNHGIALDAATATRKAFLAGVDVDMMSHYYDTQLPGLIRSGQVSMSEVDEAVR
                     RVLRVKFALGLFEHPYARGKEVTAAVAENRPLVRKAAEESLVLLQNKSAMGASPLLPF
                     TS"
BASE COUNT          858 a         1455 c         1523 g          991 t
ORIGIN      
        1 tcattctgaa ttccggatcc gtgacggtgt ccgcggaaga ggccaggctc ttcgagcggg
       61 tcctgtcgaa gggattggct ccgggtctgc tggttcgcgt gctgcgcgag atctttccgc
      121 tgctcggggc cggagccccg gtggtcgcat ccggatttgc cgaggatgac gagggctggc
      181 gggtacagcc cgcccgtgag cgaggttgat tatgtcttcc ggttcgatcc agcgctggtg
      241 cgatccgcgc gtgattctgc tggcgacgaa tcttcacgat ccgcccgacc tgatgcggca
      301 cgcggttgcg gaggccagga gaagcggggc tcgccttctg ctcgtccacg tgatcccttc
      361 ccttgcccca caggaagacg ccgccggcag ccctccccgc gtcttcctgt ttccttcgaa
      421 ggattgcgcc gccaatgctc tggaacagct ggcgctgcaa ctgcagtggc aaggagtttt
      481 atgcgaggcc gtggtgctga gaggacgccc ggccgagcag atttgcgctc tggttccgtt
      541 gcgcaaggtg gaccgtgtgt tcgtggcgag ccgcggtcgt gccgcgggcg cttcggcagg
      601 gccgtgcgtc gcggaggaat tagtgagcgc tctggcgata ccggtctgcg tcgtcagcag
      661 gcaggccgat cccgcgggga tgagcggtgc cagggcaagt cgcgtgctgc tcgcgctttc
      721 tctgcggtcg atgcggcggg agtacgtcga ctttgctttc ggggtggcca gagcccgtgg
      781 cgcgcggctc acgttgctgc atgttctcga tgggtcatcc atccgggaac ggcatcgagc
      841 gtcggcgcac gatcgagcga tgctgcgcct ggccgcgctg gctgccacct gtgacgcaag
      901 gccgcgaggg actgatctcg tcgttcgcga cggcgatcct gctgcgcaga tcgtggaaca
      961 ggccgggtgc ccttttcagg acctcatcgt tctcggatcg tcgtctctac gatcctcttc
     1021 ggccatgctg ggcggcaaca tccttcgccg ggtgattgcc gaggcccgct gtcccgtcat
     1081 cacgctcaaa ccgcccatcg cggaattccg ggcggagccc gatcccgaat ggccaattct
     1141 caggacaggt tcgttcgatt gatggcgcgt tccacgcgcc gctggaggac accatgaaat
     1201 gcgccaatcc gctctgcgag catcgtctcc actacctgcg cggcggaacg ctgcgtctgc
     1261 tggaattcga gaacgctccg gaacatcggc tgcggggcgg aggtgggggt tttccggtca
     1321 ttcagcacag cgctcggtac ttctggcttt gctcggaatg ttccgggatt ctgatcctgc
     1381 gccgctggac gcagtgcggt cttgttcttc agtcgcgcag gcagccggaa gaagcatcgc
     1441 cgctaacgtg gacggttcct gcggaaccag cggtggatgt ggagaaccgt ggttcctttc
     1501 gaattccccg cgtcaggacg gcctgaaacg cgaaagatcc aaatgggatt tcgtacacga
     1561 tctcggacaa cggcgccgga aattacgacg ccgtctgcgg cttacccgga tttcagccgc
     1621 taaagctcgc gaatccgctg caatccgtgg caatggaaat gcttctctat ccgcgagtgc
     1681 cgatggtcgt gacagctcga tccgtcgcaa ccgggtactc ctgtaaatcc cctaaccgga
     1741 gaaccgctat gtcgcttaca aagtttcgca aaattgccta cacgaccttc gtcgcaaccc
     1801 ttctgctggc actggctccc ctgctgagag cggaaagcgt tgacgattca cgcgagataa
     1861 cgcagctgct ttcccaggcg aaagcgaccg caacgctggt gacgcacgat gcagaaacgc
     1921 tcgaatcgta tacgcgttcg cggatcagct ggcaaacgca tgcgcgccaa ctggagcgga
     1981 tgcggggcca tacgaatgaa ctgattgaag acgtcaggga gctttcggcg ctgcgttcac
     2041 aaggctcgcc gtggcaacag gaagccatcg atcgcatcaa tcccatgttg aaggacatgg
     2101 ccgatcatct gaccgccacg atcgaacacc tgaacgacaa ccgctcgagt atccacttcc
     2161 agccgtatca ggactacgcc cggggcaatc ggatctttgc cgataagaca ctggtggcga
     2221 tccgcgattt tgtggactac ggtgaagccc gggccgcggc ggattcactc gaacagaagc
     2281 tgacgcttcc tgcagcaagc ggggagtgag tgcgacgtgc ctccatggtg aagaacaaag
     2341 cgggcggcgt tttgcaggag tggagcatcg ccgcccgctg ttttgggtag caggcccgca
     2401 atggggccgt cctctataat tgagtcagag tcactggcgg tccccatcca ggcttttcct
     2461 tccagggcga atcatccaca cactgcagac cggctccgtg tggtcaccta taacgtccac
     2521 aagtgccgtg ggctcgatgg acgtgtgctt ccccatcgca tccttcgtgt actcgaagag
     2581 atcgacgccg atgtgattgc gttgcaggag gtcgtctgcc gggaggaaaa atcggcggag
     2641 cgtcaccagg cccggtatct tgcggagcgc cttggcctgt tctgcgaact gggtgaaaac
     2701 cgacgccatc gcggcggagc ctacggcaat gttctgctct cgcgctatcc cattctgcac
     2761 gcgcgcaatc acgatatctc ggtcccggga cgcgaacggc gcggctgtct gcatgtcgag
     2821 ctgcaaccgg agaacagcgc ggtgctgcac atctttaacc tgcatctggg aaccgctctc
     2881 tacgaacggc gcatgcaggc gcgtcagatg tttggagaga ggctttttcc tggagccgag
     2941 ctttcgggaa acaagatcgt cctcggcgat ttcaacgagt ggacgcgagg gctgccgtcg
     3001 cgcctgttca atctgcattt cgaaagcgct cacagttaca tggatgcgca tcgcacgcgc
     3061 accttccctg gggtgctgcc catgttgccc ctggatcaca tctacttcga ccgcgaactg
     3121 cagctggacc acgtgagaat ccaccgcacc ccggctgcgc tcatggcatc ggatcatctg
     3181 ccggttgtcg cggagtttac cctgccggcg gagtctggca acgccgcggt ttccgagcaa
     3241 ggcgcggccc tgctgccgcc ttctacagac cgccttccgg aagcgcgcgt ttaagtggtt
     3301 cgcgtctctc tttcctggat ggtccgtgct tgccctcccc ggtcgtttct cgaccgcatc
     3361 ccgtacaacc cggccatccc gtccgaaact ccacccctta tgtcctgaga gtcgcagccg
     3421 tagccggcga tttccaaacc tccagcagcg aacgccagga ccaggagact ctatgccgtt
     3481 ctgttatcgc gcttcgacgc gagcgcctta ttgggttggc tttacgttga gctggatttg
     3541 cctgtgcacg ccgcttcctg cgtccgcaca aaatggagcg ccgtatgcag cacccgtggc
     3601 gaatgccggg gcggttcgcc cgtcgaatgc acagcttgct tctccggaaa tcgagcggaa
     3661 agtcgatgct ctggttcgcc agatgactct cgaagagaag ctgggacaac tggtccagta
     3721 cggcgatacc ggggactcgg caaatgctca actggcgaat gacaatcagg ctccggggaa
     3781 gaatccggag acgcacatca agatcgatcc catggaactg gcggcgacgg gccgtctggg
     3841 ttcaatgctg aatgtggtag gcgccgagcg cacgaatact ttccagcggg cggcggtgga
     3901 gaagagccgg ctgcatattc cgcttttgtt cggcgccgat atcctgcacg gctaccggac
     3961 gatctatccc atcccgctgg gtctggcggc gacgttcgac ccggacctgg tttcttcggt
     4021 ctcgcatgtt tccgcgcagg aggcgaccac tgcgggtgtt cgctggttct attcgccgat
     4081 ggtggatatc tcgcgcgacc cgcgctgggg gcgcacgcag gagggagcgg gcgaagacgc
     4141 ttatctgggc gccgcgatgg cccgcgctta cattcacggt taccagggcg aggatctttc
     4201 tcgcgctgac agcgtggcgg cgagcgtcaa gcattttgcc gcctatggcg cggcagaagc
     4261 aggccgtgag tacaacacga cggacatgtc gctgagtcgt ctgcagcagg actatcttcc
     4321 gccgtataaa gctgcggtga aggcgggcgc ggccaccatc atgagcgcct tcaatgcgct
     4381 gaacggcgtg ccatcgactg cgaatccgta tcttctgcac gatgtcttgc gcaggaactg
     4441 ggggttcgat ggatttgtcg tcagtgacta cacatcggtg atggaattgc gcaatcacgg
     4501 tatcgcgctc gatgccgcca cggccacgcg caaggcgttt ctggccggcg tggacgtgga
     4561 catgatgtcg cattactacg acacgcagct gcccgggttg attcgctccg ggcaggtctc
     4621 gatgtccgag gtggatgaag cggtgcggcg ggtgctgcgc gtgaaattcg ctctcggcct
     4681 gttcgaacat ccgtatgcgc gcggcaagga agtgactgcg gcggtagcgg agaatcgtcc
     4741 gctggttcga aaagctgccg aggagtcgct cgtgctgctg caaaacaaat cggcaatggg
     4801 tgcgtcgccc ctgcttcctt tcacgtc
//
LOCUS       DASZYN010000604         3630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_35243, whole genome shotgun sequence.
ACCESSION   DASZYN010000604 DASZYN010000000
VERSION     DASZYN010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35243"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1741
                     /locus_tag="VHE33_15605"
     CDS             <1..1741
                     /locus_tag="VHE33_15605"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HVT98931.1"
                     /translation="IRGSADFMLSHPDPELEAQIDGYIERIAAAQAIRPDGYINTYTQ
                     LAEPGHEWGLNGGLQIWQHEIYNLGALVDAGIHYYRATGKTRLLAAGIKIANTMCELM
                     GPAPKKNLVPCHPLPEEAMVRLYELFEEQPDLKGKLPLPVSESAYLALAEFWIENRGN
                     NIGKPDWIDHKASEQFVRNQEYGSGRPSWGAYAQDDAPVFEQKDIHGHAVRATLLCAG
                     IAAALRQNNDERYRQTALRLWENMVYRRMHITGGVGAFAEEERFGPDYTLPNNAYLET
                     CAAVGASFFHHNMNLAFGHARYVDELERALYNGSLPGVSLEGDSYFYQNPLEADSSRT
                     RWAWHNCPCCPPMFLKLMGAMPSYIYSTDSDSLYVNLFVGSRATTEVKGSKITVRQVT
                     RYPWDGNVRIAIEPVQPSSFSLMLRIPHWCSGERVKLNGQRIATDQRVRGYVRITREW
                     HSGDTIELDMPMPVRQVYANPLVQADVGRSMIMRGPLVYCVESADNPAVPGLRLPASS
                     RFSTRFDPAKLGGVVVIAAPGIVAQPAAQGLYAANPQAYETRSAELAAIPYYANANRG
                     PVDMAVWLPLTGS"
     gene            1753..2796
                     /locus_tag="VHE33_15610"
     CDS             1753..2796
                     /locus_tag="VHE33_15610"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HVT98932.1"
                     /translation="MKALVLTESRQFELKDVPQPVPGPGDVLIRIAGCGICGSDVHGY
                     DGSSGRRIPPLIMGHEAAGIVASAGAGATRFQRGDRVTFDSTVYCGACSFCLRGEVNL
                     CDRREVIGVACSDFRRDGAFAEYLVVPERIVYPLPASMSFAEAAMLEAVSVALHAVRV
                     SQIRGGETAVVVGAGMIGLLTLQAAFAAGCARVYIADLDDTRLRLADSLGATGTLRVE
                     DLVSEIQQLTGETGADIAFEAVGRNESIGAAIDCVRKGGTVTLIGNVVPKVDLPLQKV
                     VTRQIRLQGTAASAGEYPQAIDLVTSGKVQVKPLITAIASLADGPDWFDRLYRHEPNL
                     MKIVLAANADLMA"
     gene            2793..3587
                     /locus_tag="VHE33_15615"
     CDS             2793..3587
                     /locus_tag="VHE33_15615"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HVT98933.1"
                     /translation="MSENLFDLSGNVALVTGTSRGLGQYFARALARAGADLILTGRRR
                     ESLSSFESEIQSLGRRTLAVELDVRDPASIERMAALAEDAFGQIHILVNNAGCNVRKP
                     ALDITWDDWNLVLDTNLRGSFFVAQAIARNMIAHGYGRIINIGSVTSVFGYAGLAPYG
                     ASRGGIRQLTMSLADDWGRHGVTVNCLAPGWFRTEQNKVLYEDPRWVDYLVDRIPVKR
                     PGRPDDLDGAVIFLASEASRYITGQTLLVDGGISTGSTRATVNPQA"
BASE COUNT          683 a         1152 c         1087 g          708 t
ORIGIN      
        1 gatccggggc tcggcggact tcatgctcag tcatcccgac ccggaactgg aagcgcagat
       61 cgacggatat atcgagagga tcgcagcagc ccaggccatc cggcctgacg gctacatcaa
      121 cacctacacc caactcgcgg agccgggtca cgagtggggc ctgaacggcg gtctgcagat
      181 ttggcagcac gagatctata acctcggtgc gctcgtggac gcgggcatcc actactatcg
      241 cgccaccggt aagacccgtc tcctggccgc tggtataaaa atcgcgaaca ccatgtgcga
      301 attgatggga ccggcaccga aaaagaatct ggtcccatgc catcctctgc ccgaagaagc
      361 gatggtccga ctctacgaac tctttgagga acaaccggac ctgaagggaa agctgccgtt
      421 gccggtgagc gagagcgcct acctcgcact ggccgagttc tggatcgaaa accgcggcaa
      481 caatatcggc aaaccggact ggatcgacca taaggcatcg gaacaattcg tccgcaatca
      541 ggaatacggt tccggcagac cctcatgggg ggcctatgcc caggacgacg caccggtttt
      601 cgaacagaaa gacatccatg gtcacgcggt ccgcgcgacg ttgctgtgcg ccggcatcgc
      661 ggcggccctg cggcagaaca acgacgagcg ctatcgtcag accgcccttc ggctttggga
      721 aaacatggtc taccggcgga tgcacatcac cggcggtgtg ggcgcgtttg cggaagaaga
      781 gcgctttggg ccggactata cgttgcccaa caatgcgtat ctcgaaacct gcgccgcggt
      841 cggcgcttcg ttcttccacc acaatatgaa tctggcgttt ggccatgcgc ggtacgtcga
      901 tgagctggag cgagctctct acaacggctc actcccggga gtctcgctgg agggggattc
      961 atacttctat cagaatcccc tcgaggcgga ttcctcgcgg acgcgctggg cctggcacaa
     1021 ttgcccctgc tgtccgccga tgttcctcaa gctgatgggt gcgatgccct cctatatcta
     1081 ctcgaccgac agcgacagcc tttacgtgaa cttgttcgtc ggcagccggg cgacaacaga
     1141 agtgaagggc tcgaaaatta ccgttcgcca ggtcacacgc tatccgtggg acggaaatgt
     1201 gcgcattgcg atcgagcctg tgcagccctc gtcattttcc ctcatgctgc gcattcctca
     1261 ttggtgcagc ggtgagaggg tcaaactcaa tggtcagcgc atcgcgaccg accagcgggt
     1321 gcgcgggtat gtgagaatca cgcgcgaatg gcactccggc gacacgatcg aactcgatat
     1381 gccgatgccg gttcgccagg tttatgccaa tcctctggtg caggccgatg tgggccgttc
     1441 catgatcatg cgcggcccgc tcgtctactg cgtggagtcg gccgacaatc ccgctgttcc
     1501 agggctacgg cttcccgctt cttcgagatt cagcacccga ttcgatccgg cgaagctggg
     1561 aggtgtcgtg gtcattgccg cacccggcat cgtggcgcag cctgcggcac agggcctgta
     1621 tgcagccaac cctcaggcgt atgaaacgcg ttcggccgag ctggcggcaa tcccgtacta
     1681 cgccaacgcc aatcggggtc cagtggacat ggctgtgtgg ctgccgttga cgggttcctg
     1741 aggggcgagg atatgaaagc gctggttctc accgaatcca ggcagttcga gctgaaagac
     1801 gtgccgcaac ccgtccctgg tccaggagat gttctcatcc gcatcgccgg atgcggcatc
     1861 tgcggcagcg atgttcacgg atacgacggt tcatccggcc ggcgcattcc tccactcatc
     1921 atgggacacg aagcggcggg catcgtcgct tctgccggtg ccggggccac acgctttcag
     1981 cgcggcgatc gcgtcacctt cgattccacc gtctactgcg gcgcttgttc tttctgtctc
     2041 cgaggcgaag tgaatctctg cgaccgccgg gaagtgattg gcgtcgcgtg cagcgatttc
     2101 cgacgggatg gcgcattcgc cgaatacctc gttgtgcctg agcgcatcgt ctacccactt
     2161 cccgcctcca tgtcgttcgc cgaagccgcc atgctcgaag cggtctcggt ggccctgcac
     2221 gccgttcgag tctcacaaat ccgcggtggt gagacggcgg tcgtcgttgg tgcgggcatg
     2281 atcggcctgc tgaccctgca ggccgcattc gcggccggct gcgcgcgcgt ctacatcgcg
     2341 gacctcgacg atacgcggct tcgacttgcc gactcgctcg gcgctaccgg aaccctgcgc
     2401 gtcgaggatc tggtttctga aattcagcag ctcacgggcg agaccggtgc tgacatcgcg
     2461 ttcgaggctg taggacgcaa tgaatcgatt ggcgccgcaa tcgactgcgt tcgcaaggga
     2521 ggcacggtga cgctcatcgg caatgttgtt cccaaagtcg atcttccgct gcagaaagtc
     2581 gtcacacgcc agattcgact gcagggaact gcagcctcag ccggcgaata cccccaggcc
     2641 atcgacctcg tgacttcggg caaggttcag gtcaagcctc tcatcaccgc aatcgcctcc
     2701 ctcgctgacg gaccggattg gttcgaccgt ctctatcggc acgaacctaa tctgatgaag
     2761 attgttctcg ctgccaatgc ggacctgatg gcatgagcga gaacctcttt gacctgtccg
     2821 gcaatgtagc cctcgtcact ggaaccagtc gcggtctcgg acagtatttt gcccgcgccc
     2881 tggcccgcgc cggcgccgat ctgatcctca ccggccgacg gcgcgaatcg ctctccagtt
     2941 tcgaatctga gatccagagc ctgggccgcc gcaccctcgc cgtggaactc gacgtgcgag
     3001 atcccgccag catcgagcgc atggccgcgc tggccgagga tgccttcggt cagatccaca
     3061 ttctcgtgaa caacgcaggc tgcaacgtgc ggaagcccgc gctcgacatc acgtgggacg
     3121 actggaacct cgttctcgac accaatctgc gtggcagctt ttttgttgcg caagccatcg
     3181 cccgcaatat gatcgcgcac ggctacggcc gcatcatcaa tatcggttcg gtcaccagcg
     3241 tctttggcta tgccggcctc gctccttatg gcgccagccg cggcggcatc cgccagctca
     3301 ccatgagcct ggccgatgac tggggccggc acggcgtgac ggtcaactgt ctcgccccag
     3361 gctggttcag aaccgagcag aacaaggttc tgtacgaaga tccgcgttgg gtggattacc
     3421 tcgtcgatcg tattccggtg aagcgtcccg ggcggcccga cgatctggac ggcgctgtca
     3481 tcttcctggc gtcggaagcc agccgctata ttaccgggca gacactgttg gtcgatggcg
     3541 gcatctctac cggttcaaca cgcgcgaccg tcaatcctca ggcataacag ctcactgcgg
     3601 ccgcaggctg actcggcgga ccggcggcgc
//
LOCUS       DASZYN010000605         2408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_860376, whole genome shotgun sequence.
ACCESSION   DASZYN010000605 DASZYN010000000
VERSION     DASZYN010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860376"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VHE33_15620"
     CDS             complement(<1..717)
                     /locus_tag="VHE33_15620"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HVT98934.1"
                     /translation="MKVIQQTAHEWAVHNFKSGCEVADMLVQRLEIGPEVRDALRFTF
                     ERWNGKGFPAHAGGEAIPLPMRIVHLSHDMEAIARLFSPARAMDAARDRRDRTYDPAL
                     ADLFVTHGTEWFSKLEKTEPWDAVLELEPQPRRMLDGGDLDNALMVAADFIDLKSPFM
                     AGHSRRCAELSTEAARQFGLSDEAIITIRRAALVHDFGITGVSNSILDKRGSLTRAEF
                     DRIELHPMLTEQMLRRSPALA"
     gene            691..1047
                     /locus_tag="VHE33_15625"
     CDS             691..1047
                     /locus_tag="VHE33_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98935.1"
                     /translation="MRGLLNHLHNLIGLFPGPPCGRVRDHVMHHFGWIRSIVHQGLGA
                     NGDLIAENGGHLVGMTRAPHIAQQGNPVDGVADVLPKAGGLANPGCQQAGAKLGLQWL
                     PEGVVLRQGQGSDKFT"
     gene            1259..2392
                     /locus_tag="VHE33_15630"
     CDS             1259..2392
                     /locus_tag="VHE33_15630"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding protein"
                     /protein_id="HVT98936.1"
                     /translation="MTDSQSRNRAIRVGVIAEQTGALSFMGIADANVARMVIDDINRE
                     GGLLGHQLQLIVEDGATTDSVAAAKATKLIEQDRVDVIFGGIFSSTRQAIKGPAVEKG
                     KTLYIYPEQYEGQESHPLIFCTGPVPAQQLEPLIPWLMQKTGAKKFYLPSADYIWPHT
                     MNRKVRELVTANGGSIVGEEYFPLDHANYRRTVDDIAATGAEVVFNTIVPPGVAPFLE
                     QLHNSGFAARGGQLICTYMDENFLNLLPAAHVEGLYSCLDYYQSVTDPWSRRLLDQYN
                     KLFPGAASFTAGSGCTGMYRGLRLWAAAVKEAGSLNQVDVIRALDHARIDEAPGGPAA
                     MVPGQHHVRMNMYIAQARGGQFQVVKSLGVIEPREAPVPVSAA"
BASE COUNT          505 a          747 c          706 g          450 t
ORIGIN      
        1 cgcaagcgcg ggggatcggc gcagcatctg ttcggtgagc atggggtgaa gctcgatacg
       61 gtcgaactcg gcacgcgtga gagatccacg cttgtccaga atcgaattcg acacgccggt
      121 gataccgaaa tcatggacca gcgcggccct gcggatggtg atgatggcct cgtccgagag
      181 gccaaactgg cgggccgctt ctgtgctcag ctcggcgcat cggcggctgt gtccggccat
      241 aaagggcgat ttcaaatcga tgaagtcggc cgccaccatg agcgcgttgt ccaaatcacc
      301 gccgtccagc atgcgccgtg gctgcggttc cagttcgagc acagcatccc acggctcagt
      361 cttctccagt ttcgaaaacc attccgttcc atgggttacg aacaggtcag caagcgcggg
      421 atcataggtc cggtcgcgac ggtcccgtgc cgcgtccatc gccctggccg gggaaaacag
      481 acgcgcaatc gcctccatgt cgtggctcag gtgcacgatg cgcatgggca acggaattgc
      541 ctcgccgccg gcatgagccg gaaatccctt cccgttccat cgttcaaacg taaagcgcag
      601 cgcatcgcgg acctcgggtc cgatctccag ccgctgtacc agcatgtcgg ccacttcaca
      661 tcctgatttg aagttgtgta ctgcccattc atgcgcggtc tgctgaatca ccttcacaat
      721 ctgatcggcc tcttcccggg tccgccctgc ggtcgcgtac gcgaccacgt catgcatcac
      781 ttcggctgga ttcgcagcat cgtgcaccag ggtctgggcg cgaatggcga tctcatcgcc
      841 gaaaacggtg gccacctcgt gggcatgacc cgtgcacccc acatagcgca gcagggcaac
      901 ccagtagacg gtgtcgcgga cgtccttccc aaagccggcg gcctcgcaaa tccaggctgc
      961 cagcaggcag gagcgaagct gggactccag tggctgcccg aaggcgttgt cctgcgccag
     1021 ggccagggta gcgacaaatt cacctaaacg aagcggtttg gcaaaaatgg ggaattctcc
     1081 cgatgcaggg gctaatcagc agtgatagcg ttgctcatca atcgatcacc tttccccact
     1141 ggcggtcaag taacaagatc agcaacagcg aaggcgcgaa aactgcaccg catctgaagc
     1201 tcgtccttcg gcagccgaac ttcgatttac caaaatcaca gcttcgagga gagtttgtat
     1261 gacagattca caatcacgta accgggcgat cagggttggg gtcatcgccg agcagacagg
     1321 tgccctctct ttcatgggca tcgccgacgc aaacgtagcc aggatggtca tcgacgacat
     1381 caaccgcgag ggtggcctgc tgggacatca gctccagctg atcgttgagg acggggcgac
     1441 caccgacagc gttgccgctg ccaaagcgac gaagctcatc gagcaggacc gcgtcgacgt
     1501 catcttcggc ggcatcttca gttccacccg gcaggcgatc aaaggacccg ccgtcgagaa
     1561 gggaaagacc ctttacattt acccggagca atacgagggc caggaatctc atcccctcat
     1621 cttctgcaca ggaccagttc ccgctcagca actggagccg ctgatcccct ggctgatgca
     1681 aaagaccgga gccaaaaagt tctacctgcc ctcggcggac tacatctggc cgcacaccat
     1741 gaaccggaag gtgcgcgaac tggtgaccgc aaacggcggc tcgatcgttg gagaggagta
     1801 ttttcccctc gatcacgcga actatcgcag gaccgtcgat gacattgcag caacaggcgc
     1861 agaggtcgta ttcaatacga tcgttcctcc cggcgtcgct cccttcctcg aacagcttca
     1921 caattccggc ttcgccgcgc gaggcgggca gctcatctgc acctacatgg acgagaactt
     1981 tctgaacctg ctgccggccg cccacgttga aggtttgtac agctgtctcg actactacca
     2041 gagcgtcacc gatccctgga gcaggcgcct gctcgatcag tacaacaagc tgtttcccgg
     2101 cgctgccagc tttaccgccg gcagtggatg caccggcatg tatcgaggtc tcaggctttg
     2161 ggcggctgct gtcaaagagg cgggctcgct gaatcaggtc gatgtcatca gggccctgga
     2221 tcacgcgaga atcgacgagg cgcctggagg ccccgcggcg atggtgcccg gacaacacca
     2281 tgtgcgcatg aacatgtaca ttgcccaggc tcgcggcggt cagttccagg ttgtgaagag
     2341 ccttggcgtg atcgaaccga gggaggcgcc tgttcccgta agtgccgcgt agcggttcga
     2401 tcggcgat
//
LOCUS       DASZYN010000606         1546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_733333, whole genome shotgun sequence.
ACCESSION   DASZYN010000606 DASZYN010000000
VERSION     DASZYN010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733333"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..381
                     /locus_tag="VHE33_15635"
     CDS             61..381
                     /locus_tag="VHE33_15635"
                     /inference="COORDINATES: protein motif:HMM:NF023204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HVT98937.1"
                     /translation="MHSLCRAFAVSTLALSLTGSLALAQDRDNHDHDRDHHDNHHYVH
                     HNDWRKGHHINHDDWNRGERVEDWHAHHLRRPPAGYEYRYVDGNYVLARPDNGLIFSV
                     TIGH"
     gene            complement(453..740)
                     /locus_tag="VHE33_15640"
     CDS             complement(453..740)
                     /locus_tag="VHE33_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98938.1"
                     /translation="MRSIVRKSTRIFTAAAFAASLLSVGCAAHARVYDPYHRDYHAWA
                     GENGYYAQWETDTHRHHEDFKRRNEADRKEYWDWRHSHDHDHDHDHDHDQH"
     gene            947..>1546
                     /locus_tag="VHE33_15645"
     CDS             947..>1546
                     /locus_tag="VHE33_15645"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HVT98939.1"
                     /translation="MKTLREKLFETDRFLIAAELVSVRGSMNERTALKTRAFASDLAA
                     SSSIDWISITDNAGGNPQLAPLALGRPILYAGKEVVIHLTCKDLNRNGLESEAWMLNS
                     EGFYNILAMTGDYPVGGSEGLAKPVFDIDSVGLISLLSRMNRGLNQSSARSQPLEKTH
                     FTIGAVTSSFKLLEGELIPQYLKLRKKIECGAEFIINQIG"
BASE COUNT          294 a          580 c          363 g          309 t
ORIGIN      
        1 accaaggatt caaccatccc cctccccctc tccggaatcc aatccccccg aggagattcc
       61 atgcattccc tttgtcgagc ttttgcagtc tctaccctcg ccctgtctct gaccggctcc
      121 ctcgccctcg cgcaggaccg cgacaaccac gaccacgacc gtgaccacca cgacaaccac
      181 cactacgtcc accacaacga ctggcgcaag ggccaccaca tcaaccacga cgactggaac
      241 cgcggggagc gcgtcgaaga ctggcacgcc caccacctcc gccgcccacc tgccggttat
      301 gagtaccgct atgtcgacgg caactacgtc ctcgcccgtc ctgacaacgg cctcatcttt
      361 tccgtcacca tcggccacta atccctgaat cgattgcaaa cgcaacgagg gccggcatcc
      421 tttgccggcc ctcgttcttt tcggtgtgcg tctcagtgct ggtcatgatc gtgatcatgg
      481 tcgtgatcgt gatcatgact gtgccgccaa tcccagtact ccttgcggtc ggcctcgttc
      541 cgccgcttga agtcctcatg gtggcgatgc gtgtccgtct cccactgcgc gtagtacccg
      601 ttctcgccgg cccacgcatg atagtcgcgg tggtacggat cgtagacgcg ggcgtgcgcc
      661 gcacagccca cgctcaggag cgaagccgca aacgccgcgg cggtgaaaat ccgagtggac
      721 ttccgaacga tcgatctcat cctgttccct ttctccgccg gctttccggc agtctcatgc
      781 caggatgctc caccctccgc ccccggttgc tcccctttta aatcccgcct gcccgccgaa
      841 caggccgcat cccgtcaccc tcgtaacccc ctcccgttca gctggttttc atcccctcaa
      901 atctctcatt tacagccttc caatcatttg tttagtatcc gaacaaatga agacgctgcg
      961 cgagaagctc ttcgagaccg accgcttcct catcgcggcc gaactggtct ccgtgcgcgg
     1021 ctccatgaac gagcgcaccg ctctcaaaac gcgcgccttc gcctccgacc tcgccgccag
     1081 ttcctccatt gactggattt cgatcaccga caacgccggc ggcaatccgc agctcgcccc
     1141 cctcgcgctc ggccgcccca tcctgtatgc cggcaaagag gtcgtcatcc acctgacctg
     1201 caaggatctc aaccgcaacg gcctcgagtc cgaagcgtgg atgctgaaca gcgagggctt
     1261 ctataacatc ctcgccatga ccggcgacta tccggtcggc ggcagcgaag gcctggccaa
     1321 gccggtcttc gacatcgact ccgtcggact tatttcgttg ttgtcccgaa tgaatcgtgg
     1381 cctcaaccaa tcctccgcca ggtcccagcc gctcgaaaaa actcacttca ccatcggcgc
     1441 cgtcacctcc agcttcaagc ttcttgaagg cgaactcatc ccccagtacc tcaagctccg
     1501 gaagaagatc gagtgcggcg ccgaattcat catcaaccag atcggc
//
LOCUS       DASZYN010000607         1506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_800081, whole genome shotgun sequence.
ACCESSION   DASZYN010000607 DASZYN010000000
VERSION     DASZYN010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800081"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VHE33_15650"
     CDS             <1..544
                     /locus_tag="VHE33_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98940.1"
                     /translation="TFTARGIKLRSGQELSADIIVTATGLRMKMLGGIALVVDGQPVD
                     LSRALIYRGMMYRDIPNLASVFGYINASWTLKCELIAQRVCRLLNYMDRHAYASCTPR
                     LGEQAVPEEPMLTELTSGYVQRARDLLPRQGPDNPWKMHQNYLRDLWSLRLSSVNDGA
                     VEFARRPAVPAAQSAGAWSA"
     gene            complement(643..>1506)
                     /locus_tag="VHE33_15655"
     CDS             complement(643..>1506)
                     /locus_tag="VHE33_15655"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HVT98941.1"
                     /translation="DQGLVHLPSYRELYYRWERQQWQTQEIDFIPDRLQWEMLSEEEQ
                     QDLLYTMASFFQGEASVADALAPYVIAMPQEDMRLFVTTQLVDEARHTLFFDRFFSEV
                     LGINEGTLEQTLEVARGYLNKTMRYILVDSLLDIAERLRNAPRDLALLVEGVCLYHII
                     VEGTLALASERALLERFRQENLFPAFRGGFTAVARDESRHVIFGVTFLRQMLLEHPEL
                     ESVMLATLNKFTPLAIRGLTPPPAMIEQNLQLGFDPWLVPRYACDSLRKKLKVIGLHL
                     ELPELPPFPQA"
BASE COUNT          285 a          431 c          494 g          296 t
ORIGIN      
        1 aacgtttacg gcgcggggca ttaaactgcg ctccggccag gaattgagcg ccgatatcat
       61 cgtgaccgct acgggtctgc gcatgaagat gctgggcggc atcgcgctgg tcgtcgatgg
      121 ccagcctgtc gatctgtcca gagcgctgat ctatcgcggt atgatgtacc gcgatattcc
      181 caacctggcc tcggtctttg gctacatcaa tgcctcctgg acgctgaagt gcgaactcat
      241 cgcgcagcgc gtgtgccggc tcctcaacta catggatcgc cacgcttacg ccagttgtac
      301 gccgcgtctg ggggagcagg ccgttcccga ggagccaatg ctgacggaac tcacctcggg
      361 ctatgtccag cgcgcccgtg atctgctgcc gcgccaggga ccggacaacc cctggaagat
      421 gcaccagaat tacctgcgcg atctgtggag cctgcgtctg agttccgtaa acgacggcgc
      481 cgtggagttc gcccgtcgcc cagccgtacc cgcagcgcaa tccgccggcg cctggtcagc
      541 atgagcgatg atgcacattt tgcgctaaaa acgatgcttt tggtggacgc gcccattgct
      601 tcacgcctgg catatttttg gcgcgggtgc ttgctgagtg gctcaggcct gcgggaaggg
      661 cggcagttct ggcaattcca ggtgcaggcc gatcactttg agctttttgc gcagggagtc
      721 gcaggcgtag cgcggcacca gccaggggtc gaagcccagt tgcaggtttt gttcgatcat
      781 agccggcggc ggggtcagtc cccggatggc caggggcgtg aatttgttga gcgtggccag
      841 catgacggac tcaagttccg ggtgctccag aagcatctga cgcaggaagg tgacgccgaa
      901 gatgacgtgg cgcgattcgt cgcgggctac ggcggtaaag ccgccccgga aggccgggaa
      961 gaggttttcc tggcggaaac gctccagcaa ggcgcgttcg ctggccaggg ccagggtgcc
     1021 ttcgacgatg atgtgataga ggcaaacccc ttcgaccagg agagccaggt cacgcggggc
     1081 gttgcgcagg cgctcggcga tgtcgagcag ggagtcgacc aggatgtaac gcatggtctt
     1141 gttgagatac ccgcgcgcta cttccagcgt ttgctccagg gtgccttcgt tgatgcccag
     1201 gacttcgctg aaaaagcggt cgaagaagag ggtgtgccgg gcttcatcga ccagctgggt
     1261 ggtgacaaac agacgcatgt cctcctgggg catggcgatg acataggggg cgagggcgtc
     1321 ggcgacactg gcctcgccct gaaaaaagga ggccatggtg tacagcagat cctgctgttc
     1381 ttcctcggac agcatttccc attgcagacg atccgggata aaatcgatct cctgcgtctg
     1441 ccactgctgg cgctcccagc gataatagag ttcgcgatac gatggcagat ggaccagacc
     1501 ctgatc
//
LOCUS       DASZYN010000608         2293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_935882, whole genome shotgun sequence.
ACCESSION   DASZYN010000608 DASZYN010000000
VERSION     DASZYN010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935882"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(495..1811)
                     /locus_tag="VHE33_15660"
     CDS             complement(495..1811)
                     /locus_tag="VHE33_15660"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVT98942.1"
                     /translation="MLRKAAVLSLVAGVLAIPAFAQRDRRSDMSRLDHVFLIMMENHG
                     YQQVIDNPNEPYLNSIIKSGQVNLATNYFAVGHPSFTNYLEVVGGSNFGIRSDNAPDW
                     GSTSCTPNIASGVVNADNGGGNAPVQVESGKVCPIWGSGNDAETPAVDTWNEIAGPFI
                     ALADIDGVQAVPAAPTVGKTIGDQLVAAGLSWKSYQESLPQGSAYGVNYSNGTASNLT
                     DFTNLAPLKSSDIVQLYAVKHNPFAYFKNVQDGDDPDNSMRNTVGFDGPRGLYSDLAR
                     GEVPSLAFIVPNQCNDQHGRGNGDAFCAFDYGQNDTGYTYGTQVGLNPGLIQQGDSTI
                     ERIVTAIHRSPAWHDGRSVIVIVWDENDYSGSTTQTTGLFPSQNQNRVVLTVESNFPH
                     RQVQSDTYYNSFSLLKSMEAAFGLPCLNHACDPGVQTMSDLIGARP"
     gene            2090..>2293
                     /locus_tag="VHE33_15665"
     CDS             2090..>2293
                     /locus_tag="VHE33_15665"
                     /inference="COORDINATES: protein motif:HMM:NF014880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HVT98943.1"
                     /translation="MFENLSEKLQRAFKNLRGQGSITEENMQEALREIRVALLEADVN
                     LNVVKELIDHIREKAMGQEVMTSL"
BASE COUNT          440 a          644 c          754 g          455 t
ORIGIN      
        1 ctggcgaaac agccggacgc aggctgcgcc aatgccgcgc gagcctccgg tgatgagggc
       61 gacttttccc tcaagggaaa ggggaacagg ggacatgaaa acagagtacg cgaccgccgt
      121 atgccttgtc gggccggctc agcagaacgc gctacaccaa acgcgatggc ccggtctgca
      181 cgaggtacga ttgcgccccg tgattcggat gagtcttttg aaacagatgg ccgccctcct
      241 gacgcacaaa gaaccaggcg cctgcaaaaa atagcaagac aaccacgatc gatgcgatga
      301 cgacggccat aaaattgggg tcttttgagc gttttccggt gttcggtcgg ggtgcggcgg
      361 gatcgggcat attcggttgg atgcgggcag gggccccgga tcgttttccg cgccaccaga
      421 gaatttcatt gcgcaaaaaa agcccccggc gcggatccgg aggctctttt ctaacgtttg
      481 gaaacgacgg ccagtcaggg acgagccccg atcagatccg acatcgtctg cacgccgggg
      541 tcgcaggcgt ggttcaggca aggcagacca aaggccgctt ccatcgactt cagcagcgag
      601 aagctgttgt agtaggtgtc gctctgcacc tggcgatgcg ggaaattgga ttcgaccgtc
      661 agcaccaccc ggttctggtt ctggctgggg aagagcccgg tggtctgcgt ggtgctgccg
      721 ctgtagtcgt tctcgtccca cacgatcacg atgacgctgc ggccgtcgtg ccacgcgggc
      781 gagcgatgga tcgctgtgac gatccgctcg atggtcgagt cgccctgctg gatcagaccc
      841 ggattgagtc ccacctgcgt cccgtaggtg tagccggtgt cgttttgtcc gtagtcgaag
      901 gcgcagaagg catcgccgtt gccgcggcca tgctggtcgt tgcactggtt gggcacgata
      961 aaggcgagcg acggaacttc accgcgtgcc agatccgagt aaaggccgcg cgggccgtcg
     1021 aagcccaccg tgtttctcat gctgttgtcc ggatcgtcgc catcctgcac gttcttgaaa
     1081 taggcgaacg gattgtgttt caccgcgtac aactgcacaa tgtccgacga cttcagcgga
     1141 gccaggttcg tgaagtcggt gagattgctc gcggtgccgt tgctgtagtt cacgccgtag
     1201 gcggatccct gcggcaggct ttcctggtag gacttccagc tcagtccggc ggcgaccagc
     1261 tgatcgccga tcgtcttgcc cacagtcgga gcagccggaa cagcttggac cccatcgata
     1321 tcggcgaggg cgataaacgg gccggcgatt tcattccagg tgtcgactgc gggagtttcc
     1381 gcatcgttgc cggagcccca gattgggcaa accttgccgg attccacctg cacaggggca
     1441 ttgccgccgc cgttgtcggc attcacgacg ccgctcgcga tgtttggcgt gcaggatgtg
     1501 ctgccccagt ccggtgcgtt gtcgctgcgg atgccgaagt tcgagccacc cacgacttcg
     1561 aggtagttgg tgaagctggg atggccgacc gcgaagtagt tggtggcgag attcacctgg
     1621 ccgcttttga tgatgctgtt gaggtagggt tcgttagggt tgtcgatgac ctgctggtat
     1681 ccgtggttct ccatcatgat gagaaacacg tgatccagac gcgacatatc gctgcggcga
     1741 tcccgctgcg cgaatgcggg gattgcgagc acgccggcga caaggctcaa gactgctgct
     1801 ttccggagca tggttaacct ctttttcggg atgatttggg aatgcctgcc atggggatgg
     1861 gccgtagtta ctgtgcgggg cgcgcagaat cgggcgcatc aatggcgagt taagggaagg
     1921 tcaataaagg aagactgtga tggggcctgg gcgagatgac agaaacgaaa tctgtgccgg
     1981 cagggcgtca gcggcggacg ccccaagggc gagctgcgcg gcgcccgggc ggccctggcc
     2041 cgcgtttgcc cccgttggcc gccgtcccgg taaactgggt ttctgaccta tgtttgagaa
     2101 cctttctgaa aagctgcagc gagccttcaa gaacctgcgc gggcagggct cgattaccga
     2161 agagaacatg caggaggcgc tccgcgagat tcgcgtggcc cttctcgaag ccgacgtcaa
     2221 cctcaacgtc gtcaaagagc tgatcgacca catccgcgag aaggcgatgg gacaggaggt
     2281 gatgacctcc ctg
//
LOCUS       DASZYN010000609        11927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_608746, whole genome shotgun sequence.
ACCESSION   DASZYN010000609 DASZYN010000000
VERSION     DASZYN010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 11927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11927
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608746"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="VHE33_15670"
     CDS             <1..1180
                     /locus_tag="VHE33_15670"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HVT98944.1"
                     /translation="QRSETGKDFRVTKQVRTAVIGTGFMGRVHLEALRRTENVEVAAI
                     GGRNSAAAEKLGSAFGVPQRAPEAILSDTSIEAVHLCTPNAQHFPMAQAALQAGKHVL
                     CEKPLATSVEEAQELAALAAQKRLRNCVCHNLRYYPMVQQMRAMREAGDLGEILVAQG
                     TYSQDWLLYDTDWNWRVDSKAGGPSRCMADIGSHWFDMAEHLTGLRVSSLCADLNTVY
                     KTRKQPKGSVETFANKTLSADEVIETPIDTEDFGAVVFRLGDRARGAMTASQVSAGRK
                     NGLRVEIYGTKAGVAWDQERPDELWIGHRNSANCVIVKDPSLLKPGARGFADLPGGHS
                     EGYDDTFKQVFRRFYASIQDAGATPEYPQFVDGLRQMVLLDRELESHRTRAWVDVPAM
                     "
     gene            1229..2362
                     /locus_tag="VHE33_15675"
     CDS             1229..2362
                     /locus_tag="VHE33_15675"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT98945.1"
                     /translation="MLILAAVVAIFVYGMIAAMLGTILPELSSRFQLTPRQNGTIAFA
                     QAVGLMVASLGVGPLLDDFGKKIGLILGLGLIGVTLVLLPRSSGYRSILFLLFLLGCG
                     GGIVVTGANALTSDVGEAHRAAALNLVNIFFGLGGLATPFIAANLFKRNWVRLCYSVA
                     GLTLVALLIQVTAHMPPPTGAGRFIFADAGPVLGRPMLFLLGFLLFLYVGCEVDVWNW
                     LVRHLVEQGVPESRALNILSLGFALGLLIGRAVVSPILISVPPIYVTLAASVAMTVTT
                     YLMLRTNKTGTAAVLVFLAGLSMAPVFPTTLAIVADAFPRMTSTAIGIVITFGWAGLA
                     VSSHLIGAIAGGDPRRIRKALLVIPASAFLMVLINVGILAAAR"
     gene            complement(2359..3258)
                     /locus_tag="VHE33_15680"
     CDS             complement(2359..3258)
                     /locus_tag="VHE33_15680"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HVT98946.1"
                     /translation="MDRRTFLKNSALASGAAASTLLCGTRASWASSLEWPIGLQLYSV
                     RDSLPKDFDGTLHQLAAMGYRECEGAGFFGHSAADVKKSMSQAGLHCVSAHYSLQLLQ
                     SPDEILSYGRDLGLEYIVCSSPMPRDPAKAKGHGWAAAVESMSADDWKWIADQLNTVG
                     KRVQAAGMKFGYHNHYAEFHQHDGFLPYDVLLESTDPKLVDMEMDCGWVVIGGQKPED
                     YLTRYPDRYVMLHVKEFKLDGWKPGTPPVSTELGRGSIDYKSIFAAAKKARVKHIFVE
                     QEAFPDLPEMEAMKANADWLRAV"
     gene            complement(3330..4382)
                     /locus_tag="VHE33_15685"
     CDS             complement(3330..4382)
                     /locus_tag="VHE33_15685"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HVT98947.1"
                     /translation="MHLQEHVPLAPYTTFGIGGPARWFVEAGDEESVVEAMRFAREKN
                     QALFVLGGGSNLLVSDQGFPGLVLHIALQGIAHEGSLFRVAAGENWDSFVSHAIGAGY
                     GGIECLAGIPGTVGGTPVQNVGAYGQEVADTIASVRVLDRDNFTFSEMPASDCGFSYR
                     RSIFNSTHRDRFIVTRVDYKLRAGSKPVLQYPDLRRFFQDRPEAPSLAETAAAVREIR
                     HRKGMLLVEGESDCRSAGSFFKNPIVPQAQYDAIAARESVPVPSFPAEAGFVKIPAAW
                     LVEHAGFHKGYTLGKAGISSRHTLALINRGDARAAEIVALRDRIVQAVEGQFSIRLEP
                     EPVWLGPKASYGRPDR"
     gene            4477..4902
                     /locus_tag="VHE33_15690"
     CDS             4477..4902
                     /locus_tag="VHE33_15690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HVT98948.1"
                     /translation="MYPEIMLIPMREELTRAGIEEARTAEAVDAAVAKPGTTMLIVNS
                     VCGCAAGKMRPGVRLALRNPVVPDRSITVFAGQDREATERARSYFEGNPPTSPAVAIL
                     RDGKLVYLMQRFIIENSGPQAIAEELSRAFNELCAKQAV"
     gene            complement(5018..6169)
                     /locus_tag="VHE33_15695"
     CDS             complement(5018..6169)
                     /locus_tag="VHE33_15695"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVT98949.1"
                     /translation="MLAGFRGVRRSTPDLRGSRQLSWFIVSALFGVLLMGLRPWSPAL
                     VSVVLANFLLFAGALFIYLAATDILEMDSRVFPWPAGILIIAMPLFVWYTYANDQIFA
                     RLVIHAIVLSVIFLSASVRLFRNRRDSETRQPARACAWLLILAACLQGAWCLFESHLP
                     RNQTDFVHPDLINVGFSYLSMVLALAIVIGLLWLALTVHRQALHKMAQTDSLTSLLNR
                     RAFEEILRRDLMRSDRSGNSMGIMLIDLDYFKQVNDSLGHFAGDDVLRRIALVLRDGT
                     RPSDVLARYGGEEFVVLLRNAGVSESMIAAERIRTQIAELAGLPGRVTLTASIGVAVS
                     LPKDTATEFLLRADEALYRSKREGRNLVSLYRESDDEFETATSVYAPGT"
     gene            6455..7000
                     /locus_tag="VHE33_15700"
     CDS             6455..7000
                     /locus_tag="VHE33_15700"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVT98950.1"
                     /translation="MSQKPKSRVVLVSGPPGSGKTTLARPLAERLGFALLTKDDIKES
                     LYTSMQGTAGDVEFSRRIGSAAMEVLWALAPHCPAVVLEANFRTQSSYEREKVAGLVA
                     AGAQIVEVYCRLPREEASRRFAERAREVRHHPAHALAEMPPERMREYEEPFALGPVIE
                     VDTRMLVDEVGLAVRIRAFWE"
     gene            7117..8922
                     /locus_tag="VHE33_15705"
     CDS             7117..8922
                     /locus_tag="VHE33_15705"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HVT98951.1"
                     /translation="MHRWSKLFIPTLREAPADAEVASHKFLVRAGYIRQLGAGIYSYL
                     FLGQRSIDRIIRIVREEMDRIGQEFLLPTIHPADLWRESGRWTLMGDNMFRLKDRKGA
                     DLCLGMTHEEVITDIARNELRSYKQLPQIWYQIQTKFRDEPRPKAGLLRVRQFIMKDS
                     YSFDLDKAGLDKSYDLHDAAYRAIFTRCGLKFVVVEADSGAMGGSQSQEFMVYTDAGE
                     DLIASCAACGYAANMEKATSRLAPVNEMEATGDGMPELVSTPGQAAIADVTKFFGIEA
                     SSDIKCVAYMAKTPDVGKGDKKESASADLPVVAFLRGDHFVNETKLMAVVGATELRPM
                     VAEELERWMHGPAGYLGPVGLKPKKAVNPGVDLSAFAEDEVVVVLDPGLENRKNLVCG
                     ANKLDYHYRNVVPGRDFQWTVVADIRNAVEGEGCPQCGQPLKVARAVEVGHIFKLGDR
                     YTEKMGARVLDANGKEVTPIMGCYGIGIERILTAAVEQSNDANGFWLPRSIAPFDVVV
                     TVTNVADAGLKATGEKIASELEAAGFDVLLDDRDDRAGVKFKDADLIGIPYRVTVGKK
                     AVDGKAELVTRATASGVDVAIADLAGELRRIASVH"
     gene            9182..11821
                     /locus_tag="VHE33_15710"
     CDS             9182..11821
                     /locus_tag="VHE33_15710"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HVT98952.1"
                     /translation="MAVKVKVPKVAVRITARKALIVAGMVFGAAAVLGGLVFTFYWNK
                     YGHIVDERLKQPLFAQTAKIYAAPEEIRPGQKLTAEEIEQQLQNAGYARDGQGPASPM
                     GTYAVNAHAITIHPGPQSYHSQDGATISFDEHGVDEITGDNGQKLAAYELEPMLITGL
                     SDENRAKRRLVTYDELPKYVVPAVTSIEDRHFFRHGGVDYIRLIGAGIDDLRHAHYSQ
                     GGSTLTMQLARGFFLSPEKHIKRKLIEIIITFQLEHRFTKQQIFQMYANQVPLGQRGS
                     FAIAGFGEAAQAYFGKDVRQLDLPECALLAGIIQSPSRLNPFRHPERALERRNVVLDS
                     MVETGDITKAQAEQAKAAPLTLDPGAFDSGEAPYFVDLVRDQLVQRLGDTEYNEQGLR
                     IYTSLDPQLQDIAQAAVAAGMKHVDELVQERRERLVRLAAKAGKEPPPAHSPQVALVA
                     LNPHTGQVLALVGGRNYGISQFDHAVSHRPTGSIFKPFVYAAAFNTALAGTPLTNANG
                     TSAVFTPITLLHDEQTTFTFDNQDYTPRDFDNKYYGDITATEALYRSLNNPTIALAQM
                     VGFDNVAALARDAGIKSARGTPAMAIGAYDATPLEMAGAYTVFANDGVHIDPWMLASV
                     RAPNGDVIADYTPQMKPVLDPRAAFLTVSMMEQVLNNRYGTGAGVRNMGFVSPAAGKT
                     GTSHDAWFAGFTSNLLCIVWVGNDDYTDIKLELGANAEGARAAGPIWADFMKNAVKLP
                     EYSDTKDFVPPAGVIQVSLDNATNLLADATCPQDYTAAFLEGTQPTDTCDHSMGDQRN
                     LFQKIFGLGQRSVTQAPPPLQQAKPVAPPIQAQPAQPAVAQKQGEQQDQEEKKKKKPG
                     FWSRLFGKKDDSQQDKDKNPQEP"
BASE COUNT         2362 a         3389 c         3926 g         2250 t
ORIGIN      
        1 tcagcgttcg gagacaggaa aggattttcg ggtgacaaaa caggtaagaa cggccgtgat
       61 tggcacgggg ttcatgggac gcgttcatct ggaggctctg cgccgcacag agaacgtgga
      121 ggttgccgcg attggcggac ggaacagcgc ggccgccgag aagctggggt cggccttcgg
      181 cgttccgcag cgtgcccccg aggccatcct gagcgacaca tcgattgagg cggttcacct
      241 gtgtactccg aatgcgcagc attttcccat ggcgcaggcg gctctgcagg ctggcaaaca
      301 tgtgctgtgc gagaagcccc tggcgaccag cgtggaagaa gcgcaggaac tggcagcact
      361 ggctgcgcag aagagattgc gtaactgtgt ctgtcacaat ttgcgctact atccgatggt
      421 gcagcagatg cgcgccatgc gcgaggcggg agatctgggc gagattctgg tcgcgcaggg
      481 aacctactcg caggactggc tgctgtatga cacggactgg aactggcgcg tggattccaa
      541 agccggcggc ccctctcgct gcatggccga cattggctcg cactggttcg acatggctga
      601 acacctcacc gggctgcgcg tttcctcgct gtgcgccgat ctgaacacgg tgtacaagac
      661 gcgcaagcag cccaagggct cggtagaaac gttcgcgaat aagacgctgt cggccgatga
      721 ggtgatcgag acccccatcg atactgagga ttttggggcg gttgtgtttc gtctcggtga
      781 tcgggcgcgc ggtgcgatga cggcgagcca ggtctctgcc ggccgcaaga acgggctgcg
      841 cgtggagatt tacgggacga aggcgggcgt tgcgtgggac caggagcggc ccgatgagct
      901 gtggattggg catcgcaaca gtgcgaactg cgtgatcgtg aaggacccgt cgctcctgaa
      961 acctggagcg cgcggctttg ccgatctgcc gggcggccac agcgaaggct atgacgacac
     1021 attcaagcag gtgtttcggc ggttctacgc atcgattcag gatgcgggcg cgacgccgga
     1081 gtatccgcag ttcgtcgatg ggctgcggca aatggtgctc ctcgacaggg aactggagag
     1141 tcaccgaacg cgcgcctggg tcgatgttcc cgcgatgtga cgcggtgcct gtttttattt
     1201 ctcaaccaga acttcgaagg agccttgatt gctgatcctc gccgcggtgg ttgcgatttt
     1261 tgtctacggc atgattgccg cgatgctggg gacgattctt cccgagttgt ccagccggtt
     1321 ccagctcacg ccgcggcaga acggcacgat tgcttttgcg caggctgttg ggttgatggt
     1381 cgcttcgctc ggcgtaggtc cgctgctcga cgacttcggc aagaagatcg gactgatcct
     1441 cggcctcggc ctcatcggcg tgactctggt cctgctgcca agatcttcgg gctatcgcag
     1501 catcctgttt ttgctctttc tgctgggatg cggcggaggc attgttgtaa ccggggctaa
     1561 cgctctcacc agcgatgtcg gggaagcgca ccgcgcagcg gcgctgaacc tcgtcaatat
     1621 tttctttggg ctgggcgggc tggccacgcc cttcattgct gcgaatctct tcaagcggaa
     1681 ctgggtccgg ctttgttaca gcgttgctgg actgacgctg gtggcccttc tgattcaggt
     1741 gacggcgcac atgccgccgc cgacgggcgc agggcggttc atcttcgcgg acgcgggtcc
     1801 ggtgctcggt cggcccatgc tgttcctgct cggattcctg ctgtttctgt atgtcggctg
     1861 cgaggtcgat gtgtggaact ggctggtgcg ccacctggtg gagcaaggtg tgccggaatc
     1921 ccgggcgctg aacatcctgt cgctgggatt tgcgctcggc ctgctgatcg gacgcgcggt
     1981 ggtttcgccg atcctgatca gcgtgccgcc gatctacgtc actctggccg cttctgtagc
     2041 gatgactgtt acaacctacc tgatgctgcg gaccaacaaa acgggaacag cggcagtgct
     2101 ggtgtttctc gccggcctgt cgatggctcc ggtgtttccg acgaccctgg ccatcgtggc
     2161 ggatgcgttt ccgcgcatga catcgacggc gatcgggatc gtcatcacct tcggctgggc
     2221 cgggctggcc gtgagttcgc acctcatcgg cgccattgcc ggcggcgatc ccaggcgcat
     2281 tcgcaaggcg ctgctggtca ttcccgcgtc agcgtttctg atggtgctga ttaacgtggg
     2341 gattctggct gcggcgcgtt aaacggcgcg cagccagtcc gcgttggcct tcatcgcttc
     2401 catctccggc aggtcgggga aggcttcctg ctccacgaag atgtgcttga cgcgcgcctt
     2461 tttggctgcg gcaaagatgc ttttgtagtc gatgctgccg cgtccaagct ccgtggaaac
     2521 gggaggggtg ccgggtttcc agccgtcgag tttgaactcc ttgacgtgga gcatgacgta
     2581 gcggtctgga tagcgcgtca ggtagtcctc tggcttttgc ccgccgataa cgacccagcc
     2641 gcagtccatt tccatatcga ccagcttcgg gtcagtgctc tcgagcagga cgtcgtaggg
     2701 caggaagccg tcgtgctggt gaaactcggc atagtggttg tgataaccga acttcatgcc
     2761 cgccgcctga acgcgtttgc cgacggtgtt gagctgatcg gcaatccact tccagtcgtc
     2821 ggcactcatc gattcgacgg cggcggccca tccgtggccc ttcgcttttg ccggatcgcg
     2881 gggcattgga gacgagcaga cgatgtactc aagtcccagg tcgcggccat agctgagaat
     2941 ctcgtccggt gactgcagca gctgcaggga gtagtgcgcg ctcacgcaat gcagaccggc
     3001 ctgggacatc gacttcttca cgtccgcggc cgaatgaccg aagaagccgg cgccctcaca
     3061 ttcgcggtag cccatagcgg cgagttggtg gagggttccg tcgaaatcct ttggcagaga
     3121 atcgcggacg gagtagagct ggagcccgat gggccattcc aggctactcg cccaggatgc
     3181 ccgtgtaccg cagagaaggg tggaggctgc cgcaccggaa gcgagcgcgg agttcttcag
     3241 aaaggtcctt cgatccatgg ccgccattcg aacacaggga ctggctgggt gcaaaccagg
     3301 gacagagttt agagttgaga gtttagagtt tagcggtccg gtcggccgta tgaggccttt
     3361 gggccgagcc agacgggctc gggctcgaga cgaatgctga attgcccttc caccgcctgg
     3421 acgatgcggt cgcggagtgc gacgatttcg gcggcccggg catcgccgcg attgatcagt
     3481 gcgagcgtgt gacgggagga gataccggcc tttcccagcg tgtatccctt gtgaaaaccc
     3541 gcatgctcga ccagccaggc tgctgggatc ttgacgaagc cggcttctgc aggaaaggag
     3601 ggaacaggaa cggattcgcg agcggcgatt gcatcgtatt gagcctgggg gacgatgggg
     3661 ttcttgaaga agctcccggc gctgcggcaa tccgactcgc cttcgaccag aagcatgcct
     3721 ttgcggtggc gaatttcgcg gacggcagcc gcggtttcgg ccagcgatgg agcttcaggt
     3781 cgatcctgga agaaacgccg gaggtcggga tactgcagaa cgggtttgga gcccgcgcgc
     3841 agcttgtagt cgacgcgcgt gacgatgaag cgatcccgat gagtggagtt gaagatgctg
     3901 cgccgatagg agaagccaca atcggaggcg ggcatctcgg aaaaggtgaa gttgtcgcgg
     3961 tcgagtacac ggacggaagc gatcgtgtcg gcgacttcct ggccgtaggc gccgacgttt
     4021 tgcacgggtg ttccgcccac ggtgccggga attccggcaa ggcattcgat gccgccgtag
     4081 ccggctccga ttgcgtgaga gacgaagctg tcccagttct cgccggcggc gactcggaac
     4141 aggctgcctt cgtgcgcaat accctgaagg gcgatatgca ggaccaggcc ggggaagccc
     4201 tggtcggaaa cgagcagatt gctgccgccg ccgagcacga agagcgcctg gttcttttcg
     4261 cgggcgaatc tcatggcctc gacgaccgat tcttcgtcgc cggcttcgac aaaccatcgc
     4321 gcggggccac cgataccgaa ggtcgtatac ggggcgagag gtacgtgttc ctggaggtgc
     4381 atcttagaga gatataccag cacggtggca ctgcccccct gcggaccgta caatagagtt
     4441 cagatcgtta cgttttctac ggaggagacc cgccgaatgt atcccgaaat catgctcatc
     4501 cccatgcgtg aggagttaac ccgcgcgggc attgaggagg cacgtacagc cgaggccgtc
     4561 gacgccgctg ttgccaagcc cggtacaacg atgctgatcg tgaactccgt ctgcggctgc
     4621 gcggcgggca agatgcgtcc cggagttcgc ctggcgctgc gtaatcccgt ggttcctgat
     4681 cgttcgatca ccgtgtttgc cggacaggac cgtgaagcga ccgagcgagc ccggtcttac
     4741 ttcgagggca atccgccgac gtcgcctgcg gtggcgatcc tgcgggatgg caagctggtg
     4801 tatctgatgc agcggttcat catcgagaac agcggaccgc aagccatcgc cgaggagctt
     4861 tcgcgcgctt tcaatgaact gtgcgcaaag caggccgtct agaggttttg ctgtatgcga
     4921 gaaggctgcc cctgggcagc ctttctttct gcgggaagga aaaatacagg tccgttcgtc
     4981 ttgccgagcg ttgctccgcg gtcgctctgg atgacactca ggtgccagga gcgtaaacgc
     5041 tggtggctgt ctcgaactca tcgtcggact cgcggtagag gctgaccagg ttgcggcctt
     5101 cgcgcttgga gcggtacagc gcctcgtcgg cgcgcagcag gaactcggtt gccgtgtctt
     5161 tcggcaggct cacggcgacg ccaatgctgg ccgtcagggt cacccggcca ggcagccccg
     5221 cgagttcggc aatctgggtg cggatacgct cggcggcgat catcgactcg gagacgccgg
     5281 cattgcgcag caggacgacg aattcttcgc cgccgtaacg cgccagcaca tccgaaggtc
     5341 gggtcccgtc gcggaggacg agggcgatgc ggcggaggac gtcatcaccg gcgaaatgac
     5401 cgagggaatc gttgacctgc ttgaagtaat cgaggtcgat gagcatgatg cccatggagt
     5461 taccgcttcg gtcgctgcgc atcagatcgc ggcggagaat ctcctcaaag gcgcgccggt
     5521 tgagcaggct ggtgagggaa tcggtttggg ccattttgtg cagcgcctgg cggtgcacgg
     5581 tcagagccag ccagagcagg ccgatcacga tggcgagggc gaggaccatc gagaggtagc
     5641 tgaagcccac attgatcaga tcgggatgga cgaagtcggt ctggtttctg ggcaaatgcg
     5701 attcaaagag gcaccatgcg ccctgcaggc aggcggcgag gatcaggagc caggcacagg
     5761 cacgcgcggg ctggcgggtt tccgaatccc ggcgattacg aaacagccgg accgatgcgg
     5821 agaggaaaat gacgctcaga acgatggcat gaattaccag gcgggcgaat atctggtcgt
     5881 tggcgtacgt gtaccagaca aacaggggca tggcgatgat gagaatgcct gccggccacg
     5941 ggaagacgcg cgaatccatt tcgaggatgt cggtggcggc gagatagatg aagagtgcgc
     6001 cggcaaacag cagaaaattg gcgagcacaa cgctcaccag ggccggactc cagggacgga
     6061 gccccatcag cagaacgccg aacaatgccg aaacgatgaa ccacgagagc tgacggctgc
     6121 ctcgaaggtc gggagtgctg cgccggacgc cccgaaatcc tgcgagcata agaagaagag
     6181 ccgctgcata accgcacatt aaaaagggcg tatacatggg tgctgcgttc acacctccac
     6241 gaaccagaca gctcgccacc acgaaggcat cgagccggtt cctccgagct cagcgacggg
     6301 ccaacatcac cggggtaatt aggtgcaatc aggatggcat acgcctacat ccaaagtcac
     6361 cacaaaaggt gactgagaac cccgttctac atggctattc tgtgtcagtg gaaataaccc
     6421 aatcagtatt atccaggtgg ctcagccggg accgatgagt caaaaaccga aaagccgtgt
     6481 tgttctcgtc tccgggcctc ctggttccgg taagacgacg ctggcccggc ctctggccga
     6541 gcggctgggc ttcgctctgc tgaccaagga cgacatcaag gaaagtctct acacatccat
     6601 gcagggaacg gcgggcgatg ttgaattctc gcgtcgcatt ggatcggcgg caatggaggt
     6661 tctgtgggct ctggcgcccc actgtccggc ggtggtactg gaggcaaatt ttcgaacgca
     6721 aagcagctac gaacgagaga aggtagcggg cttagttgcc gcgggagcgc agatcgtgga
     6781 ggtctattgc aggctgccgc gcgaggaggc atcgcggcgc ttcgcggaac gggcgcgcga
     6841 ggtgagacac catccggctc acgcgctggc agagatgccc ccggaacgga tgcgggaata
     6901 tgaggagccg tttgcgctgg gtccggtgat cgaggtggat acgagaatgc tcgtcgatga
     6961 ggtgggactc gcggttcgga tcagggcatt ctgggaataa caccgcgaac cgcaccagtg
     7021 gtcgcgccca tttagaatgg tgcctggcac tcattcatct tcctgcaagc gcttctccgg
     7081 ggccggaggt gctcgcacaa aggcgcattc gcacgcatgc atcgctggtc gaagctcttc
     7141 attcccaccc tgcgcgaagc tcccgcagac gccgaagttg ccagtcacaa attcctggtg
     7201 cgcgccggat atatccgcca gctcggagcg ggaatttact cctacctgtt cctcgggcag
     7261 cgttccatcg accggatcat tcgcatcgtt cgcgaggaga tggatcgcat cgggcaggag
     7321 ttcctgttgc cgacgattca cccggccgat ctgtggcgcg aatcggggcg ctggaccctg
     7381 atgggcgaca acatgtttcg gctgaaggac cgcaagggtg cggacctgtg cctcggcatg
     7441 acccacgaag aggtgatcac cgatatcgcc cgcaatgagc tgcgcagcta caagcagctg
     7501 ccgcaaatct ggtatcagat ccagaccaag tttcgcgatg agccaaggcc gaaggcgggt
     7561 ctgctgcgcg tgcgccagtt catcatgaaa gacagctact cctttgatct ggacaaagcc
     7621 ggactcgata aaagctatga cctgcacgat gccgcctatc gcgccatctt cacgcgctgc
     7681 gggttgaaat tcgtggtcgt ggaagcggat tcgggcgcaa tgggcggctc gcagtctcag
     7741 gaattcatgg tttacaccga tgcgggcgaa gatctgatcg cgagctgcgc ggcgtgcgga
     7801 tatgcggcca acatggagaa ggcgacctca cggctggcgc cggtcaacga gatggaggcg
     7861 accggggatg gcatgccgga gctggtctca acgccaggcc aggcagcgat tgccgatgtg
     7921 acgaagttct tcggcatcga ggccagctcg gatatcaagt gcgtggccta catggcgaag
     7981 acacccgatg tgggcaaagg cgacaaaaag gaatcggcca gcgcggacct cccggtggtg
     8041 gcgtttctgc gcggggacca ctttgtgaac gagaccaaac tcatggctgt ggttggtgca
     8101 accgagttgc gaccgatggt ggccgaagag ctggagcgat ggatgcacgg ccccgcgggc
     8161 tatctgggcc ccgttggtct caaaccgaag aaagctgtga atccgggtgt tgacctttcg
     8221 gcatttgcgg aggacgaggt agtcgtcgtg ctcgacccag gacttgagaa ccggaagaat
     8281 ctcgtctgcg gggcgaacaa gctcgactac cactacagga atgtcgttcc ggggcgtgat
     8341 ttccagtgga cggtggtggc cgatatccgc aacgcggtcg agggcgaagg ctgtccgcag
     8401 tgcggccagc cgctcaaagt cgcgcgagcg gtggaggtgg gacacatctt caagctcggc
     8461 gatcgctaca cggagaagat gggtgcccgg gtccttgatg ccaacggcaa ggaagtgacc
     8521 ccgatcatgg gctgctatgg catcgggatc gagcgcatcc tgacggccgc cgttgagcag
     8581 agcaatgacg ccaacggctt ctggctgccg cggtccattg cccccttcga cgtggtggtg
     8641 acggttacca atgttgccga tgctgggctc aaagccacgg gagagaaaat tgcatctgag
     8701 cttgaggctg ccggcttcga tgtgttgctc gacgaccggg acgaccgtgc aggggtcaag
     8761 ttcaaggacg ctgacctcat cgggattccc tatcgggtga ccgtcggcaa gaaagccgtc
     8821 gatggcaagg ccgagttggt aacccgcgcg accgcctcgg gagtcgacgt agccattgcg
     8881 gacctcgccg gggagctgag gcggatcgcc tcggtacact agaagaggag gcgcatccgc
     8941 tgccgtcgtt gccgaaaatg tgtcaggcgg cacacatcgt gtcacccgcg acacgacgag
     9001 tgggagcgga aatgcgacga agatgcgtgt tttcaatgca tccaataagg gctttgcgcg
     9061 tgcatctgtg cagtgcgaag cctgacggcg agcgtgtact gcgatctgca ctgaggctca
     9121 ggagacgggc cctttgcaga ccggactcgc gtgaacaggc agggaaggcg aatcaatttc
     9181 tatggcggta aaagtcaaag ttcccaaagt ggccgttcgc atcaccgcaa gaaaggcgct
     9241 catcgtagcg ggaatggtct tcggtgcagc ggctgtgctg ggcgggctgg ttttcacctt
     9301 ttactggaac aagtacgggc atatcgtcga cgagcggctg aagcagccgc ttttcgcgca
     9361 aacggccaag atttacgccg ctcctgaaga gattcggcca ggacagaagc tcaccgccga
     9421 ggagatcgag cagcagttgc agaatgcggg atacgcccgc gacggccagg gtccggcttc
     9481 tcccatgggc acctatgccg tgaatgcgca tgccatcacc atccatcccg gcccgcagtc
     9541 ataccactcc caggacgggg cgacgatcag ctttgacgag cacggggtgg atgagatcac
     9601 cggcgataac ggccagaagc tcgccgccta cgaactggag ccgatgctga tcacaggcct
     9661 gtcggatgag aaccgggcca agcgccgact ggtgacctac gacgaactgc cgaagtatgt
     9721 ggtcccggcc gtgacgtcga tcgaagaccg gcatttcttc cgccatggcg gagtggacta
     9781 tatccggctg atcggcgccg gtatcgacga tctgcgccat gctcactaca gccagggcgg
     9841 atcgacgctg accatgcagc tggcgcgcgg cttcttcctt tcgcctgaaa aacatatcaa
     9901 gcggaagctg atcgagatca tcattacttt ccagctggaa caccggttta cgaagcagca
     9961 gatcttccag atgtatgcca atcaggtgcc gcttggacag aggggcagct tcgcgattgc
    10021 cggcttcggc gaggcggccc aggcctactt cgggaaggac gtccgacagc tcgatctgcc
    10081 cgaatgtgcg ctgctggcgg gcataatcca gagccccagc cgcctgaatc cattccgtca
    10141 tccggagcga gcgctggagc gccgcaacgt ggtgctggac tcgatggtgg agaccggcga
    10201 catcacgaaa gcccaggccg agcaggcaaa ggccgcgccg ctgaccctcg acccaggcgc
    10261 gtttgattcc ggtgaagcgc cctacttcgt ggatctggtc cgagatcagc tggtccagcg
    10321 gttgggcgat acggagtaca acgagcaggg cctgcgcatc tacacgtcgc tcgatcctca
    10381 gctccaggac attgctcagg cggctgtggc cgctggcatg aagcacgttg acgaactggt
    10441 gcaggagcgg cgtgagcggc tcgttcgtct ggcagccaaa gcagggaagg aaccgccgcc
    10501 tgcgcactcg ccgcaggttg cgctggtagc gctgaatccg cataccgggc aggttctggc
    10561 gctggtgggt ggccgcaatt acgggatcag ccagttcgat cacgctgtgt cgcatcggcc
    10621 gacgggctcg atcttcaagc cgtttgttta tgcggctgcc ttcaacacgg cacttgcggg
    10681 gacaccgctg acgaatgcga atggcacgag cgcggtgttt acaccgatta ccctccttca
    10741 cgacgaacag accaccttta ccttcgacaa ccaggactac acgccgcgcg attttgacaa
    10801 taagtattac ggcgatatca cggctaccga agcgctgtat cgctcgctga acaatccgac
    10861 gatcgcgctg gcgcagatgg tgggctttga caacgttgcc gcgctggctc gcgacgccgg
    10921 catcaagtcc gcgcgaggca cgccggcgat ggcgattgga gcctacgacg ccacgccgct
    10981 ggagatggcg ggtgcctaca ccgtgtttgc gaacgatgga gtgcacatcg atccatggat
    11041 gctggcttcc gtgcgtgcgc cgaatggcga tgtgatcgcc gattacacgc cccagatgaa
    11101 gccggtgctc gatccgcgcg ccgcgttcct gaccgtgagc atgatggaac aggtgctcaa
    11161 caatcgttat ggcacgggcg cgggtgtgcg caacatgggc ttcgtttccc cggcggcggg
    11221 taaaaccgga acctcgcacg acgcctggtt cgccggattc acaagcaatc tgctgtgcat
    11281 tgtctgggtt ggcaacgacg actacaccga catcaagctc gaactcggag caaacgcgga
    11341 gggtgcgcgc gctgccggac caatctgggc cgacttcatg aaaaacgcgg tgaagttgcc
    11401 ggaatactct gacaccaaag actttgtacc tcctgccggc gtgatccagg tgagcctgga
    11461 taacgcgacc aacctgctgg cggacgccac atgcccgcag gattacacgg ccgcgtttct
    11521 cgaggggacg cagccgactg acacctgcga tcactcgatg ggagaccagc ggaacttgtt
    11581 ccagaagatc tttggtttgg ggcagcgatc ggttacgcag gcgcctccgc cgctgcagca
    11641 ggctaagccg gtggcgcctc cgattcaggc tcagccggca cagccagcgg tggcgcaaaa
    11701 gcagggcgag cagcaggatc aggaagagaa aaagaagaag aagcctggat tctggtctcg
    11761 tctgttcggc aagaaggacg acagccagca ggacaaggac aagaatccgc aggagccatg
    11821 aaattcagcg tcgccaccca tcgcaagcgc gagattgttg acatcaccga acaggtggag
    11881 tcgcacttgc ggaatgcaac cggcatctgc tgcctcaacg tactgca
//
LOCUS       DASZYN010000610         6828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_733676, whole genome shotgun sequence.
ACCESSION   DASZYN010000610 DASZYN010000000
VERSION     DASZYN010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6828
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733676"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..625
                     /gene="fabZ"
                     /locus_tag="VHE33_15715"
     CDS             68..625
                     /gene="fabZ"
                     /locus_tag="VHE33_15715"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HVT98953.1"
                     /translation="MENSDNILEGRQVLEITEIMGILPHRYPFLLIDRVVEIERKKRI
                     VAIKNVTINEPFFQGHFPGYPIMPGALVVEAIAQTGGVLLMPEVPDRDKKLMVFTSIE
                     RAKFRKPVVPGDQLRIEVSVLTWKPRVAKMEGRATVDGKLACEATVMCALVPRTQEAG
                     GAADADPALVSGAAADSAGGSVKAE"
     gene            622..1398
                     /gene="lpxA"
                     /locus_tag="VHE33_15720"
     CDS             622..1398
                     /gene="lpxA"
                     /locus_tag="VHE33_15720"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HVT98954.1"
                     /translation="MSIHPTAVVAPGAVIPESCTVGPWCTVGPNVVLGEECELISHVV
                     LDGHLTAGARNRFSPFACVGVAPQDLKYKGEPTGVTLGDDNTIRECVTISRGTVGGGG
                     MTRIGSGCLLMAYVHVGHDSVIGDHCILANNATLAGHVTVEDYVTVGALSPIHQFCRV
                     GKHAYIGGGTVVTQDILPFSLTSATREARAYGMNKVGLERRGFDRDRLRALHHAFRTL
                     LASKLNTAQAAEKLRAEGIASEDVRYLLEFVEGSKRGVIQ"
     gene            complement(1726..2415)
                     /locus_tag="VHE33_15725"
     CDS             complement(1726..2415)
                     /locus_tag="VHE33_15725"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HVT98955.1"
                     /translation="MKRSLSLVCLLASGLSVAAIAQAAAPANSDPAPAAAAAVPPSAS
                     TRIAIIQFQGAVAQTNEFQRDLADLRKKYEPQEAKIQADNQEVETLKKQLQDGGANVS
                     DVERQNKMRTIDDKTKTLQRAAQDLQSNEQQDGQETFNQVANKVGEVMISYAQQQGFN
                     LVLDAGQQNSNVLWATPSTDITKAVIEAYNAKSGVPAPPTPVPSAPAPRSTTPGATHT
                     TPRTPPATPHK"
     gene            2776..3156
                     /locus_tag="VHE33_15730"
     CDS             2776..3156
                     /locus_tag="VHE33_15730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HVT98956.1"
                     /translation="MKTRHFRDLLVWQKSMALAAEIYALTERFPRREIFGLSAQMRRS
                     AVSVPSNIAEGHGRLSDRNLAVFLGHARGSLFELECQTGLARQLSFADAGSARKIVAD
                     INEVARMLNALLSKVQPNAATTRP"
     gene            complement(3243..6077)
                     /gene="bamA"
                     /locus_tag="VHE33_15735"
     CDS             complement(3243..6077)
                     /gene="bamA"
                     /locus_tag="VHE33_15735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HVT98957.1"
                     /translation="MVLLLAPLLPLPRLMAQASAPASSNDVGRKIVEIRVIGSRRIPK
                     ETIQARMYSHVNDAYDPLTVERDFNSLWNTGYFEDVRIEKEDTPQGVILDVYVREKPT
                     IRDITYKGLSSVTESDVLDRFKKEKVGLSQESQYDPARIAKAVTVIKQMLAEHGHQFA
                     TVRPEVRTIPPASVQLIFQIKEGPTVKVGKITFSGNQHVSSRTLREAMRNLRPIGIPH
                     SIILENIFARTYDATKLDEDTERVRQAYRDRGYLRGGPAGPAETKIRNEGGLSLFTFR
                     PRKGKRIDIHMNIEEGDRYRLAAINFSGYKPGTNVKALRGLFTQKDGAIFDLTTFQKD
                     LDNLRKAYSQNGYINFSPVPNPTFDDANHTVTWDINIDEGKQFTVSRIEFQGNTVTRD
                     FVIRRELLLQEGQVYNSHLWELSLLRLNQLDYFNPLKVEQDSETHQNTEAGTVDLLLK
                     VQEKGKNSIGLNGGVSGLNGAFLGLNYQTNNFLGLGETLSVQANAGSLSRNLLFAFNE
                     PYFRNKPLNLGFQVFDRKTDYNASKSYSFAGNVTANESSAVSSLLQNYNQGDKGFNFS
                     VSYPIRHFNLFGFSRVGATYAWDSSSVRAFSQASSNLFQTLAFRSGIQGQNALEGIIT
                     SSAMFSYNSSNIDSNYLPHTGRSLSATVQLAGIWGSVRYVRPVVEYKQFTPMKGLWFN
                     KAGRNTLGMRLQAVYITGFSGDVAPPFDRPSAGGESDIRGFDVRTITPYGFVPTRVLF
                     DLTNPDGSTVPRDPTNPNLGPIQVPLPVYGVASIGGDTNFTWNTQYMIPIYGHTVGFN
                     IFNDFGMDMAISKDQLRQSPEGIDALNSPLYGCPNYVNGTCQGGQRIKFSTTIDPIKG
                     TNYVPRDSVGAEIDVMMPIINAPFRIYYAVNPLKLFTTFSRTDLITRSMFPAGGAGDY
                     SYAEANQFYNGLYVLREPSKTFRLTVATTF"
     gene            complement(6385..>6828)
                     /locus_tag="VHE33_15740"
     CDS             complement(6385..>6828)
                     /locus_tag="VHE33_15740"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HVT98958.1"
                     /translation="AFVPAPESRHSDASERYPLELLARKADNYMNSTFPNLEGHRKME
                     SAYNGVLELHAEDATPRGISNGDIVDVFNDRGALSLRAHVNGSVRPGVVAARLNWNKL
                     SAGGHNVNLLTSQRLNDLAGGPTFYSVLVDVRPAAASHQNRSDLR"
BASE COUNT         1281 a         1687 c         2478 g         1382 t
ORIGIN      
        1 accgcttcat ctgctggccg gactttcgcc gacgggctga ccgccattta taatcggcgc
       61 tgcgcaaatg gaaaactccg acaacattct ggaagggcgg caggtgctcg agatcaccga
      121 gatcatgggc attttgccgc atcgctatcc gtttctgctg atcgaccggg tagtggagat
      181 cgagcggaag aagcggattg tggcgatcaa gaacgtcacg atcaacgagc catttttcca
      241 ggggcatttt cctggttatc cgattatgcc aggagcgctg gtcgtggagg cgattgcgca
      301 gacgggcgga gtgctgttga tgccggaagt tccggatcgc gacaagaaac tgatggtgtt
      361 caccagcatc gagcgggcga agttccgcaa gccggtggtg ccgggagatc agttgcggat
      421 cgaagtgagc gtgctgacgt ggaagccgcg cgtagcaaag atggaagggc gggccacggt
      481 ggacggcaaa ctggcctgcg aggcaacggt gatgtgcgcg ctggtaccgc ggacgcagga
      541 ggcggggggc gccgcggatg ccgaccccgc gctggtgagc ggagcagcgg cagactcagc
      601 gggcggatcg gtgaaggcgg agtgagcatt catcctacgg ccgtggttgc gccgggggcg
      661 gtgattccgg aatcgtgcac cgtgggaccg tggtgcacgg tggggccgaa tgtggtgctg
      721 ggcgaagagt gcgagctgat ttcgcatgtg gtgctcgacg ggcatctgac cgccggcgcg
      781 cgcaaccggt tttcgccgtt tgcatgcgtg ggggtggcac cgcaggatct gaaatataaa
      841 ggcgagccga ccggcgtcac gctgggcgat gacaacacga ttcgcgagtg cgtgacgatc
      901 tcgcgcggga cggtgggggg cggcgggatg acgcgcatcg ggagcggctg cctgctgatg
      961 gcctatgtgc atgtggggca cgacagcgtg atcggggacc actgcattct ggccaataac
     1021 gctacgctgg ccggacatgt gacggtggaa gattatgtga cggtgggcgc actgtcgccg
     1081 atccaccagt tctgccgcgt aggcaagcac gcttatatcg gcggggggac ggtggtgacg
     1141 caggacatac ttcccttctc tctgacgtca gcgacgcgcg aggcacgcgc gtatgggatg
     1201 aacaaggtgg ggctggagcg gcgcgggttc gatcgggaca gactgcgggc gctgcatcat
     1261 gcgtttcgaa cgctgctggc ttcgaagttg aatacggcac aggcggcgga gaagctgcgc
     1321 gcggagggga tcgcgtcgga ggacgtgcgg tatctgctgg agtttgtgga ggggtcgaag
     1381 cgcggggtca ttcagtagaa aaacgggtcg ggaatggacc tgggattgcg gctggcattc
     1441 actggaggtt catgcggttt tgtggaaaac aaaaaaggat gccccttcac acgttacgac
     1501 agtgcataac tggggaccgt ctccgtaact caatgagaaa acgggagatg gagcgagcag
     1561 tagcgttatt cgctctgtaa agtgggatgt cgagatagtt aagagcgttc gccgcagaaa
     1621 tcttccacag aaatttccac agcgcgggcc ggttgtctgc ggggtcccga acgcgagggt
     1681 gatggggggc tcttctgtac aagaagagcc ccgattttcg cagggttatt tgtgcggggt
     1741 cgcgggcgga gtgcgcggcg tcgtgtgtgt cgcgccaggc gttgtcgacc gcggagcagg
     1801 agccgagggg accggagtcg gaggagcggg aacgccggac ttggcgttgt aggcttcgat
     1861 cacagccttg gtgatgtcgg tggaaggggt agcccagagg acattgctgt tctgctggcc
     1921 cgcatcgagg acaaggttga agccctgctg ctgggcgtag gagatcatca cttcgccgac
     1981 tttattggcg acctgattga aggtctcctg cccgtcctgc tgctcgttgc tctggaggtc
     2041 ctgggcagcg cgctgaaggg ttttggtctt gtcgtcgatg gtgcgcattt tattctggcg
     2101 ctcgacgtcg ctgacgttgg ccccgccgtc ctggagctgc ttcttcaggg tctcgacttc
     2161 ctggttgtcg gcctggatct tggcttcctg gggctcgtac ttcttgcgga ggtcggcgag
     2221 gtcgcgctgg aactcattgg tctgagcgac agcgccctgg aactggataa tggcaatcct
     2281 tgtggaggcg gaagggggga cggcggcagc agcggcaggc gccggatccg aattggccgg
     2341 ggcagcggcc tgggcaatgg ctgcgacgct cagtccggac gcgagcaggc aaacgagtga
     2401 caacgaacgc ttcatggaaa tgagaaactc cttcggtttc ctggtgacct ggcccgcatt
     2461 tctcgctctg cggaaattcc ggttccctga attcgaggat cgggatcggg ctcaggttct
     2521 cgcccgggat gcaaaacgcg gacagagatt ttctcaggaa cctgggcaaa ttataggtcc
     2581 ggggaatggg gcagtcaacg cttgaggaca gctgaaaagc ggtcagctgg tgaagcggtc
     2641 agctaaagtc cggtcggctg gtgaagcggt cagcgatggt ccgtcagctg agaccggtca
     2701 gccaacaggc ggccaggggg gcacaaaact ctggggatct tgcggcaggg ggcgacccga
     2761 ggtccgatag tcagcatgaa aactcgccat tttcgggact tgctggtttg gcagaaatcg
     2821 atggcgttgg ccgccgagat ctatgcactc acggagcgct ttcccagaag agagattttt
     2881 ggcctgagcg cgcagatgcg acggagcgcc gtctcagtgc cgagcaatat tgctgaggga
     2941 catggcaggc tcagcgaccg caatctcgca gtgtttctcg ggcacgcccg cggctcgctc
     3001 ttcgaactcg aatgccaaac aggtttggcg aggcagctaa gtttcgcgga cgctggatct
     3061 gctcgaaaga tcgtggcaga tatcaatgaa gttgcccgga tgctcaatgc attgctttct
     3121 aaggttcaac cgaatgcagc gaccacgagg ccgtgatcgc tgctttgcgg actccgctga
     3181 ccggttctta gccgaccgct tcaccagctg accggccttt agctaaccgg ttcccagcta
     3241 tctcagaaag tggttgccac cgtcaggcgg aaggtcttgc tgggttcgcg gaggacgtag
     3301 aggccgttgt agaactggtt ggcctcggca taggaatagt cgcccgcccc gccggccggg
     3361 aacatgctgc gggtgatgag atcggtccgc gagaaggtcg tgaacagctt gaggggattg
     3421 accgcatagt agatgcggaa gggcgcgttg atgatgggca tcatcacatc gatctcggcg
     3481 ccgaccgaat cacgcggcac gtagttggtg cccttgatgg gatcgatggt ggtgctgaat
     3541 ttgatccgct ggccgccctg gcaagtgccg ttgacatagt tggggcagcc atagagcggc
     3601 gaattgagcg cgtcgatgcc ttcggggctc tggcggagct ggtccttact gatcgccata
     3661 tccatgccga agtcgttgaa gatattgaaa ccgacggtgt gcccgtagat cggaatcatg
     3721 tactgcgtgt tccaggtgaa gttggtgtcg ccaccgatgg aggctacgcc gtagacgggc
     3781 agaggaacct ggatgggccc gagattcggg tttgtgggat cgcgcgggac ggtggaaccg
     3841 tcagggttgg tgaggtcgaa caggacacgc gtcggcacaa agccgtaggg cgtgatggtg
     3901 cggacgtcga agccgcggat gtcggattcg ccaccagcgg agggacgatc gaagggcggg
     3961 gcgacatcgc cgctgaagcc ggtgatgtag acggcctgga ggcgcatgcc gagcgtgttg
     4021 cggccagcct tgttgaacca caggcccttc atgggagtga actgcttgta ctcgacgacc
     4081 gggcggacgt agcgaacgct gccccagatg cctgccagct gcacggtggc ggagagggat
     4141 cgtccggtgt ggggcaggta gttgctgtcg atgttgctgg agttatagct gaacatcgcc
     4201 gaactggtga tgatgccttc gagggcgttc tgaccctgaa tgccgctgcg gaaggcgagg
     4261 gtctggaaga gattgctgga ggcctggctg aaggcgcgga ccgaagagct gtcccaggca
     4321 tacgtggcgc cgacgcgcga gaagccgaag aggttgaagt ggcgaatggg gtagctgacg
     4381 gagaaattga atcccttgtc gccctggttg tagttctgca gcagcgatga gacggccgag
     4441 ctttcgttgg cggtcacgtt gccggcaaac gagtagctct tggaagcgtt gtagtccgtc
     4501 ttgcggtcga agacctggaa gccgagattg agcggcttgt tgcggaagta aggctcgttg
     4561 aatgcgaaga gcaggttgcg ggaaaggctg ccggcgttgg cctggacgct gagagtttcg
     4621 ccgagtccga ggaagttgtt ggtctggtaa ttaaggccga ggaaggcgcc gttgaggccg
     4681 ctgacgccgc cattgaggcc gatggagttc ttgccttttt cctggacctt gaggaggagg
     4741 tcgacggtac cggcttcggt gttctggtgg gtctcggaat cctgctcgac cttcagcgga
     4801 ttgaagtagt cgagctgatt gaggcgcagg aggctcagct cccacaggtg gctgttgtaa
     4861 acctggcctt cctggagcag cagttcgcgg cggatgacaa agtcgcgggt gacggtgttg
     4921 ccctggaatt cgatgcgcga gacggtgaac tgcttgcctt catcgatatt gatgtcccac
     4981 gtgacggtgt ggttcgcgtc gtcgaaggtg gggttcggga ccgggctgaa gttgatatag
     5041 ccgttctggc tgtaagcctt gcggagattg tcgagatctt tctggaaggt ggtgagatcg
     5101 aagatcgcgc catccttctg ggtaaagaga ccgcgcaggg cctttacatt agtgccgggc
     5161 ttgtagccgg agaagttgat ggcggcgagg cgatagcggt ctccttcctc gatattcata
     5221 tggatatcga tacgcttgcc ttttcgcgga cggaaggtga agagggagag cccgccttcg
     5281 ttgcggattt tggtctccgc cggtccggcg ggaccgccgc gcagatagcc gcggtcgcga
     5341 taggcctggc gaacgcgctc ggtatcttca tcgagcttgg tggcgtcgta agtgcgggcg
     5401 aagatgtttt cgaggatgat ggagtgcgga ataccgatgg ggcgcaggtt gcgcatggct
     5461 tcgcgaaggg tgcgcgagct gacgtgctgg ttgccggaga aggtaatttt gccgaccttg
     5521 acggtggggc cttccttgat ctggaagatg agctgaacgg aggccggggg gatggtgcgg
     5581 acttcggggc gcacggtggc gaactggtga ccgtgctcgg cgagcatctg cttgatcacg
     5641 gtgacggctt tggcgatgcg agccggatcg tactggcttt cctgggagag gccgaccttc
     5701 tcctttttga agcggtcgag gacatcggac tcggtgacgg aactcaggcc cttataggtg
     5761 atgtcgcgga tggtgggttt ctcgcggacg taaacgtcga ggatgacgcc ctggggcgtg
     5821 tcctctttct cgatgcggac gtcttcgaag tagccggtgt tccagaggga gttgaaatcg
     5881 cgttccacgg tgagcgggtc gtaggcatcg ttcacgtggc tgtacatgcg cgcctggatg
     5941 gtttctttgg ggatgcggcg gctgccgatg acgcgaatct cgacgatctt ccggccgaca
     6001 tcgttggagg aggccggcgc ggacgcctgg gccatcagcc gcggaagggg cagcagcggg
     6061 gccaggagaa gaaccaaaat cagggagcgc ctgaggatgc ggcgcagatt cctgcatggt
     6121 acgtgcgtgg agccagcgct ccagagattc acggaagaaa taggcacacc ctcactgctc
     6181 gaaaactcgc ggtcgttggg aaaaggcgat tatatcagcg tgattcacgc gggcggcggc
     6241 acgaacggga gcgcggaagg cgatgtgtgg ggcacaccag agagtcgatt tcggcggcgg
     6301 cggcaataac agcatcatcc tcgtctcgcg tcccgcaggg ctccgcctgc tgaggacaaa
     6361 accattgcag aaggtgggat cagtttatcg aagatcgctt cgattttggt gggacgcagc
     6421 agcggggcga acgtcaacga ggacggagta aaaggtgggt ccgccggcga gatcgttgag
     6481 ccgctgcgag gtgagcaggt tgacgttgtg tccgcctgcg gagagcttgt tccagttgag
     6541 gcgcgccgcg acaacgccgg ggcgcacact gccgttgacg tgagcgcgga gggagagagc
     6601 gccgcgatcg ttgaagacat cgacgatgtc gccgttggag atgccgcgcg gggtggcatc
     6661 ctcggcgtgg agctcgagga cgccgttgta cgcggactcc atcttccggt gtccctcgag
     6721 attggggaag gtggagttca tatagttgtc ggctttgcgc gcgagcagct cgagcggata
     6781 gcgttcgctg gcgtcggagt ggcgcgattc gggtgcagga acgaaggc
//
LOCUS       DASZYN010000611         7155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1250374, whole genome shotgun sequence.
ACCESSION   DASZYN010000611 DASZYN010000000
VERSION     DASZYN010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250374"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VHE33_15745"
     CDS             complement(<1..648)
                     /locus_tag="VHE33_15745"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HVT98959.1"
                     /translation="MMSPIPQPGRRRLTRREFVCSTALGLVGSSSLARALSAEPTQPW
                     FEAIPAERSGIVWTHTAGLSADMYLPETVGAGCAFFDYDNDGWMDIYLVNSGPCDFYT
                     PAKPLRNALYRNNRDGTFTDVTEKAGVPGNAYGMGVAVGDYNGDGLPDLYVTQYPHSI
                     LYRNNGDGTFTDVTAKSGLAAPGWATSAVWFDYDNDGRLDLFVCRFADFSKEKNVW"
     gene            complement(645..1598)
                     /locus_tag="VHE33_15750"
     CDS             complement(645..1598)
                     /locus_tag="VHE33_15750"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT98960.1"
                     /translation="MTVRQTAKSWSLLCAGALSGGMILFAQTQAAGTSVQQGIRLVEA
                     GRCAEALPLFERGIPHITDKAVRYRAQMAEVRCALAVDDRQLAADTLFRLQRESPGNP
                     EVLYVQTHMFSELAMRAAGELQQKNPSSYQARRLEAEGLESQGKQEQAAAVYRDILKD
                     HPSVPGIHYRLGQIALAEAGDAGPTEAAKAEFEKEIAVDPTNASAEFVLGELARRKGD
                     WNEAIGHFTRATHLDAGFSEAYLALGMSLAASGRYADAKPPLEHYVQLAPDDPAGHYQ
                     LAIADTHTGDQAGARREMALQAQAAARSKSMDATQGHPVQQ"
     gene            complement(1611..4358)
                     /locus_tag="VHE33_15755"
     CDS             complement(1611..4358)
                     /locus_tag="VHE33_15755"
                     /inference="COORDINATES: protein motif:HMM:NF039503.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5695 domain-containing protein"
                     /protein_id="HVT98961.1"
                     /translation="MFSMACLSSSAAPQQPASKKPPAPGPMLSQGFLTLDTPQFTLKL
                     VRSSQTVAALLPRNARDFDFTPGDQLVARSRNGFYHLGDIDLRLRTGAADTWQDYSTA
                     LAREPIQALPATGSVLASANLASTLPPTIPLAIIRTWALDRGQLILRFTLRNRTQQAV
                     EIGSLGIPMIFDNIMTDRTLAQAHAICSFDDPYIGEDAGYLQVTRLSGHGPALLVVPE
                     SHTPFEAWNPILGERPGNLEMAHDPTPRGITFEGFYEWMVHSAAYQQNEWKNAQPWNA
                     PTSFTLQPGQSRVYALRFVLADSIPGIEKALAANRRPVAVGVPGYILPMDMEGHLFLK
                     SGEPVRSMKVDPDGAIEVRKADPTPGGWSHYLLRGETWGRARLTIRYANGLVQTIAYR
                     VIKPESEAVADFGRFLTTEQWFADSADPFHRAPSAITYDGQEHQQVTQDSRVWIAGLS
                     DEAGAGSWLALAMKELIAPDPKEVAQFEQFADHVLWGGIQYSDGPQEYGVRKSLFYYQ
                     PDQLPPNFYNSSFDWKTWASWSRKASEAVDRSYNYPHVASAWWVLYRLARNHVGLVKD
                     HPWQWYLTHAYETSMAMSRLAPYYAKFGQMEGDVFLYILEDLKREGMTSEADRLEAAM
                     RQRAEHWNTEEYPFASEMPWDSTGQEEVYTWTKYFGFTAKAQTSIDAILGYDPTIPSW
                     GYNGSARRYWDFVYGGKVRRLERQLHHYGSGINAVPLLSAYRGDPSNLYMLRVAYGGT
                     MGELSNIDQNGFGSAAFHAFPDMLAGDPYSGDYGSGFWGFAVNTATYVVHDPVLGWLC
                     FGGNLEENHGKVEVTPLDAARSRVYIAPADLWITLDAGSIRSVEFDSHTGAVRLALDA
                     ASGITPTAWLRLEQPATLPGLNHYQPLQRLSRERGAWAVPLKSSPVEIVLQKPPTRPH
                     E"
     gene            complement(4564..>7155)
                     /locus_tag="VHE33_15760"
     CDS             complement(4564..>7155)
                     /locus_tag="VHE33_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98962.1"
                     /translation="QPGNNAPTFPGSSFANQTAGNQTRNFTGSFGFDYIFSPALINQL
                     KAGYLYDATSFAYNAAPLYATEPSVNWNYPGATGNMSGQTYQAPINTYYPIWDFSDSV
                     TLQHGKHTLQAGLSWYKEQDHYWNAPGGFYIYDLANSDGSGLATGDPAQNAFTNSGAS
                     PTLPGASQADVQRAQSLYAILTGRLSDVNGENSYDIKTKTYAKTGTLSEYPLDEISSA
                     WSFFGEDSWRVTPTLTLNFGLRWDIFGAEKDLTGFYHSADEASIYGPTAVGDLFHPGS
                     LKGNQNPTISVHAQPYKPWKVTPQPAFGFAWNPHASSGALKALLGGDQTVIRGGYALR
                     RFTEPYQYFWDYATDYGQFYYQQFNLIANNTGTQGTFAPGSLSLGDTLPSFILSPQAY
                     ESTSPEAEFTYTGGPGVNGIQPNLDEPTAESWNFGIQRGLGHSMALEVRYNGNHTLHQ
                     WIAIDPNEVNIFENGFLKEFQNAQANLAASGGTSFSSTYGNATPTFDAAFGGATASDY
                     TNSQYINYLNTGQAGAMAGVLSGINGTVPYFCNLVGAGFTPCATNAGYTGAGSGYPIN
                     FFQANPYSGGDSTGELSSHGYSNYNGLQVDLRQGSWHGLQYDANYTWSHTLGVESPNS
                     WTGSFNAFTLRNLRQSYAPAVFDIQNAFHASGTYDIPVGHGRTYLAHNRAVDAALGGY
                     TVGTIVTWQTGAPAYLAGGYHTFNDYGDGGIQLHGATPSQLQHAIGVHEVPGHTFADL
                     IDPKYLRAVTGGGANSTYITPNITPGTFGRIDYIHGPRQFYQDLEVTKLFPIHEAMTF
                     NIQASFINVWNHPVFGYANGFGPAPNGAASSFQSSIQSSGFGQGSPTNEGTGFGRIIE
                     LRGNFTF"
BASE COUNT         1453 a         1997 c         2243 g         1462 t
ORIGIN      
        1 ccagacattc ttctctttgc tgaaatcggc gaaacggcac acaaagaggt ccagtctgcc
       61 atcgttgtcg tagtcaaacc agacagcgct ggtcgcccac ccaggagccg ccagcccgga
      121 cttcgcggtc acgtcggtga aggtgccgtc gccgttgttg cgatacagaa tgctgtgcgg
      181 atattgcgtg acgtaaaggt cgggcaggcc gtcgccgttg taatcgccga ccgcgacgcc
      241 catgccgtaa gcgttgccgg gcactcccgc cttttcggtc acgtcggtga aggtgccatc
      301 tcgattgttg cgatagagcg cgttgcgcaa cggctttgcg ggagtgtaaa agtcgcacgg
      361 gccgctgttg accaggtaga tgtccatcca gccgtcgttg tcgtagtcga agaaggcaca
      421 gcccgcgccg accgtttccg gcaggtacat gtcagctgag aggccggcgg tgtgagtcca
      481 gacgatgccg ctgcgctcgg caggaatggc ctcgaaccat ggctgcgtgg gttctgcaga
      541 cagtgcgcgt gccagcgaag aagaacccac caggcccagc gcggtgctgc aaacgaattc
      601 tctgcgggtg agacggcgtc gacctggctg gggaatcggg gacatcattg ctgaacggga
      661 tgtccctgcg tggcatccat ggatttcgag cgcgcggcgg cttgcgcctg cagagccatt
      721 tcacgacgag cgcctgcctg atcgccggtg tgtgtatcag cgatagcaag ctgatagtgc
      781 ccggctggat catcgggcgc gagctgaacg tagtgctcaa ggggcggctt cgcgtcggca
      841 taacggccgc tggctgcgag agacatcccg agcgccagat acgcctcaga aaaaccggca
      901 tcaaggtgag tggcccgtgt gaagtgcccg attgcctcgt tccagtctcc tttgcggcga
      961 gcgagttctc cgagcacgaa ctcagcagaa gcattagtgg ggtcgacagc aatctccttt
     1021 tcgaattccg ccttcgccgc ctccgtcggg cctgcatcgc ccgcttcagc aagtgcgatt
     1081 tggcccagcc ggtagtgaat gccgggaacg ctgggatgat ccttaaggat gtcgcggtag
     1141 acggcggcgg cctgttcctg tttaccctgc gattcaaggc cctcagcttc cagtcggcga
     1201 gcctgatacg agctgggatt cttttgttgc aactccccgg cggcccgcat ggccagctca
     1261 gagaacatgt gggtctggac gtagaggacc tctggattgc ctggagattc tcgctgcagt
     1321 cggaagagtg tatcggccgc aagctgacgg tcatcgactg ccagggcgca gcgaacctct
     1381 gccatttgcg cgcgataacg aacggccttg tctgtgatgt gcgggatccc gcgttcgaaa
     1441 agcggcagtg cctccgcaca ccgcccggcc tcgacaagac ggattccctg ctggacagaa
     1501 gtgcccgcgg cctgcgtctg cgcaaaaagg atcatgcctc cggaaagagc gccggcgcag
     1561 agaagcgacc aactcttcgc tgtctgtcgg acggtcacga ctgcgctccc tcattcatgg
     1621 ggtcgcgttg gcggcttctg aagcacgatc tcaacagggc ttgacttgag cggaacggcc
     1681 caggcgccac gttcgcggga gagcctctgg aggggctgat agtggttgag accgggcaag
     1741 gtagcaggct gctcgaggcg cagccaggct gtcggtgtaa tcccgctcgc cgcatcgaga
     1801 gcgaggcgca ccgctccggt atgagaatcg aactcaacgc tgcgaatgct cccggcgtcg
     1861 agcgtgatcc agagatccgc aggagcgatg tagacgcgac tgcgagcagc atcgagaggc
     1921 gtaacttcga ccttgccgtg gttctcctca agattgcctc cgaagcaaag ccatcccaga
     1981 accggatcgt gcacgacata ggtcgccgtg ttaaccgcga atccccaaaa gccgctgcca
     2041 tagtctcccg aatacggatc gccggcgagc atgtcgggga acgcgtgaaa ggcggccgag
     2101 ccgaatccgt tctggtcgat attactcagc tctcccatgg ttccgccata ggcgacgcgc
     2161 agcatataga gattcgaagg atcgccgcga tacgcggaga ggagcggcac agcattgatg
     2221 cccgagccat agtgatgcaa ctggcgctcc agacggcgga ccttgccccc gtacacgaag
     2281 tcccagtagc ggcgcgcact gccgttatag ccccagctcg gaatcgtcgg atcataaccg
     2341 agaatggcat caatgctggt ctgcgctttg gcggtgaatc cgaaatactt tgtccaggtg
     2401 tagacctctt cctgacccgt ggagtcccag ggcatctcgc tggcgaacgg atactcctcg
     2461 gtattccagt gttcggcacg ctgccgcatg gcggcctcca gccgatcagc ctcgctggtc
     2521 atgccttcgc gctttaaatc ctcaaggata tagaggaaga catcgccctc catctggccg
     2581 aacttcgcgt agtacggcgc gagccgggac atggccatcg aggtttcgta cgcatgcgtc
     2641 aggtaccact gccagggatg atctttcacc aaaccgacat ggttgcgtgc gagccggtac
     2701 agaacccacc acgccgaagc aacatgtgga tagttgtacg agcggtcgac cgcttcggaa
     2761 gccttccggc tccaactcgc ccaggtcttc cagtcaaagc tgctgttata gaagttgggt
     2821 ggcaactggt caggctggta atagaacagg ctcttgcgaa cgccgtattc ctgagggcca
     2881 tcactatact gaatgccacc ccagagcaca tgatctgcaa attgctcgaa ttgtgcgacc
     2941 tccttcggat cgggtgcgat cagctctttc atcgcgagag ccagccatga acccgctcct
     3001 gcttcatcgc tgaggccggc gatccagaca cgactgtcct gggtcacctg ttgatgctcc
     3061 tgaccgtcgt acgtgatggc agaaggcgcg cggtgaaagg gatcggcgga gtcggcaaac
     3121 cactgctcgg tggtgagaaa acgcccgaag tcggcgacgg cctcggattc cggtttgatt
     3181 acccggtagg cgatcgtctg tacgagacca ttagcgtagc ggatcgtgag ccgggcgcgt
     3241 ccccacgtct ctccgcgcaa aaggtaatgg ctccagccgc cgggcgtcgg gtctgctttt
     3301 cgaacttcga tcgcgccatc tggatcgacc ttcatcgagc gcacgggctc acctgatttc
     3361 agaaaaagat gcccctccat atccatcgga aggatgtagc cgggcactcc caccgcgacc
     3421 gggcgacggt tggcggcgag agccttctca atcccaggaa ttgagtccgc gagaacgaac
     3481 cgcagtgcgt agacacggga ctggccaggt tgcagtgtga agctggtggg tgcgttccag
     3541 ggttgagcat tcttccattc gttctgctgg taagcggcgc tgtgcaccat ccattcatag
     3601 aaaccctcga aggtgatccc gcgcggcgtt ggatcgtgag ccatctcgag attccccggt
     3661 ctctcgccga ggattggatt ccacgcttca aaaggcgtgt ggctctccgg caccaccagc
     3721 agggctggtc catgaccgct aaggcgagtt acctggagat acccggcatc ctcgccaatg
     3781 tagggatcgt cgaagctgca gatggcgtgc gcctgagcga gggtgcggtc tgtcatgatg
     3841 ttgtcaaaga tcatcggaat cccgagggag ccgatctcga ccgcctgctg cgttcggttg
     3901 cggagggtaa agcgaaggat tagctgcccg cgatccaggg cccacgttcg aataatcgcg
     3961 agcggaatag tcggcggcag cgtcgacgcc aggttcgctg aggcaagaac gctccctgtg
     4021 gcgggaagtg cctggatcgg ctcgcgggcg agcgcagtgg agtaatcctg ccaggtgtcg
     4081 gcagcgccgg tacgcagccg gagatcgatg tcgcccaggt ggtagaaacc gttcctggag
     4141 cgtgctacga gctgatcgcc cggagtgaag tcaaaatctc tcgcattcct gggcaggagc
     4201 gccgcaacgg tttgggagga gcgaaccagc ttcagcgtga actgcggggt atcaagggtg
     4261 aggaaaccct gactgagcat cgggccggga gccgggggtt tcttcgacgc cggttgttga
     4321 ggagccgcgc tcgacgacag gcacgccatt gaaaacatga acatcgcaag cagcgagcat
     4381 tgaactcgcc ggcgcgagga cccatgtctt tgctcaggcg gaaacaggat tggacatctg
     4441 cagagtttca agcattcctc gtccgtttgc tgacaatccc tgatcggaac gcatcacgac
     4501 tgcgcgagtt gaaaaaaaca ccggacgcac gtgaggcgtc cggcgttgct cacgagggct
     4561 cacttagaaa gtgaagttac ctcgtaactc gataatgcga ccgaatccgg tcccctcgtt
     4621 cgtcgggcta ccttgcccga atcctgagct ttgaatgctg gactggaagg atgaggcagc
     4681 tccgttcggg gcgggcccaa agccgttcgc atacccaaag accggatgat tccagacatt
     4741 gatgaaagaa gcctggatgt tgaaggtcat ggcttcgtgg atggggaata gcttggtcac
     4801 ctccaggtcc tgatagaact gtcgcggccc gtggatgtag tcaatccggc cgaaggttcc
     4861 cggagtgatg tttggcgtga tgtatgttga attcgcgccg ccaccggtca cagccctcag
     4921 gtatttgggg tcgatcaggt cggcaaaagt gtgcccgggg acttcgtgta caccgatggc
     4981 gtgctgcagt tggctcggcg tcgctccatg taactggata ccgccgtccc catagtcgtt
     5041 gaaggtgtgg tagccgccgg cgaggtatgc cggggcgccg gtctgccagg tcacgatggt
     5101 tcccacagtg tacccgccca aggcagcgtc taccgctctg ttgtgggcca gatatgtccg
     5161 tccatggcca actggaatat cgtaggtgcc gctggcatgg aaggcattct gaatgtcgaa
     5221 caccgcgggt gcgtagcttt ggcggagatt gcgcagagtg aaggcattga atgatccggt
     5281 ccatgagttg ggcgactcaa caccgagagt atggctccag gtgtagttgg cgtcgtactg
     5341 cagaccgtgc cacgagccct ggcgaaggtc aacctgcaga ccgttgtaat tggagtagcc
     5401 atgggaggaa agctctccgg tcgaatcgcc tccggaatag gggtttgcct ggaagaaatt
     5461 gatcggatag cccgagcccg cgcctgtata ccccgcattt gtggcgcatg gtgtaaatcc
     5521 ggcgccaacc agattgcaaa agtagggaac agtgccgttg atgccggaaa ggacaccagc
     5581 catcgcacct gcctggcccg tgttcaggta gttgatgtac tgcgaattgg tgtagtccga
     5641 tgcagtagca ccaccaaagg cagcgtcaaa ggttggtgtt gcgttgccat aagtgctcga
     5701 gaacgacgtt ccgcctgacg ccgccaggtt agcctgagca ttctgaaatt ccttcaaaaa
     5761 tccgttttcg aagatattca cttcgttggg atcgatggca atccattggt gaagcgtgtg
     5821 attgccgttg tagcggacct ccagtgccat agaatggccc aggccgcgct gaatgccaaa
     5881 gttccaggat tcagcggtgg gttcgtccag gttcggctgg atgccgttga cccctggtcc
     5941 acccgtatag gtaaactccg cttcgggcga ggtcgattcg tacgcctggg gtgacagaat
     6001 gaagctgggc agcgtgtcgc ccagggagag actgccagga gcaaatgtcc cctgcgttcc
     6061 agtattgttc gcgatcagat tgaactgctg gtagtagaac tgcccgtagt cggtcgcgta
     6121 gtcccagaag tactggtacg gctcggtgaa gcgccgcagt gcgtaacctc cgcggatgac
     6181 ggtttggtcg ccgccgagaa gggctttcaa cgcgccgctg gaagcatggg gattccaggc
     6241 aaagccaaaa gccggttggg gtgtcacttt ccacggcttg tagggttgag catgaacgga
     6301 aatcgtggga ttctgattgc ccttgagcga acccgggtgg aacaggtcgc ccacggcggt
     6361 gggaccgtag atggaagcct catcggcgga gtggtagaag cccgtcaggt ctttttctgc
     6421 gccaaagata tcccagcgaa ggccaaagtt gagtgtcaaa gtcggagtta ctcgccacga
     6481 atcttccccg aagaagctcc acgccgaaga tatttcatcc agtggatatt cgctcagtgt
     6541 gccggtcttg gcgtaggtct tcgttttaat gtcgtaggag ttttctccgt tgacatcgct
     6601 gagtctcccc gtcaggatcg cataaaggga ctgtgccctc tgcacgtccg cttgggaggc
     6661 accgggaagc gttggactgg cgccggagtt ggtgaaggca ttctgcgccg gatcgcccgt
     6721 agccagacca ctcccgtcgc tatttgcgag gtcgtagata tagaaacctc ccggggcgtt
     6781 ccagtagtga tcctgttcct tgtaccagga gaggccggcc tgcaaggtgt gctttccatg
     6841 ctgcagcgtc acggagtcag aaaagtccca gatggggtaa taggtgttga tcggggcctg
     6901 gtaggtttgg ccggacatat tgcccgtcgc gccaggataa ttccagttca cgctcggctc
     6961 ggtcgcgtaa aggggtgccg cgttataggc aaacgaggta gcgtcataga ggtaacccgc
     7021 cttcaactgg ttgattagag cgggtgaaaa gatgtaatcg aatccaaaag aaccagtaaa
     7081 gttccttgtc tgatttccag cagtctgatt ggcaaaactg ctgccgggaa aggtgggcgc
     7141 attgtttccc ggctg
//
LOCUS       DASZYN010000612         1989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_481397, whole genome shotgun sequence.
ACCESSION   DASZYN010000612 DASZYN010000000
VERSION     DASZYN010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481397"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VHE33_15765"
     CDS             <1..688
                     /locus_tag="VHE33_15765"
                     /inference="COORDINATES: protein motif:HMM:NF024096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HVT98963.1"
                     /translation="TKRAFDFGHAAHRFVLGEGEDIVPVDADDWRTKAAKEQAESARA
                     EGKVPLLRAEFDRALAMANALQDNPGAAALFKSGVAERAMYSTTGPPAEVQLRGKADW
                     LTHLNGRLTIVDYKTAASADPATFERAATKFKYHLQAVWYRDLAYTLELSEDPDFVLV
                     AQEKTPPYLSSVMRFDDIAIFEGHALKRQAIDTYARCLQTGEWPGYPGGTQIIHVSPW
                     AYSEEQEVEV"
     gene            1239..1811
                     /locus_tag="VHE33_15770"
     CDS             1239..1811
                     /locus_tag="VHE33_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98964.1"
                     /translation="MNIDDTIIPDSTQLNAEDMLGGPVTVTITSVDRGTAEQPVNIYL
                     DQFSGRAYRPCKSMRRVLVAAWGSDAAKYIGRRMTLFNDPSVKWAGIQTGGIRISHMS
                     HIDHKLTLALTLTRGKRAPFVVEPLPDAPPPISDEDAADFARCIAESSTVAELDSVAT
                     ALKSCDLGSHRKRLQTAWAERKAALETEAQ"
BASE COUNT          457 a          607 c          568 g          357 t
ORIGIN      
        1 cactaagcgc gccttcgact tcggccacgc tgcgcatagg ttcgtcctcg gtgaaggtga
       61 agacatcgtg cccgtggatg ctgacgactg gcgcaccaaa gccgcgaagg agcaagccga
      121 gtccgcacgc gccgagggga aggttccgct gctgcgtgct gagttcgaca gggcgctcgc
      181 tatggctaac gcgctgcagg acaaccctgg cgcggcggca ttgttcaaat ctggtgttgc
      241 cgaacgcgct atgtactcca ccaccggccc gccagccgaa gtgcagttac gaggcaaagc
      301 ggactggctg acccatctaa acggtcggct caccatcgtg gactacaaga cagcagccag
      361 cgccgaccca gccacctttg aacgcgccgc gacaaaattc aagtaccacc tgcaagccgt
      421 ctggtaccgc gaccttgcct acacactaga gctcagcgaa gacccggact tcgttctcgt
      481 cgcacaggaa aagacgccgc cgtacttgtc atcggtaatg cgcttcgacg acatcgccat
      541 ctttgagggc cacgcactta aacgccaggc tatcgacacc tacgcgcgat gcctacaaac
      601 cggcgaatgg cccggatacc caggcggcac acagattatc cacgtctcgc cgtgggccta
      661 ttcagaggag caggaggtgg aggtctaatg gcgcccagaa aacggcttgc tgttgatcgg
      721 ttcctggaaa aggtgctgta tctagacaac ggatgccatg agtggaccgc gtatgtcgga
      781 gtgaatggct acggcaggtt ttacctagaa ggcaaagggg cgttggcgca tcggtggtca
      841 tatgaacact tcatcggccc cattccgacc ggtctggtga ttgaccacct ttgtcgaaac
      901 acaaagtgtg taaacccgaa acaccttgag ccagttacag cttccgagaa tgtacggcgt
      961 ggtctcgggc ctgcagctgc agcggatcga atgcttcgcg tgacccactg cccccagggc
     1021 catccataca ccgatgcgaa cacctacagg gacagccgcg ggcgcgcttg ctggacgtgc
     1081 aagcgcgcga atcgaaagaa gcattacgcg cttaataggc agcgcgcgat tgaacgtgca
     1141 gcagagtggc ggcgcgccaa cccggagcgt tatcgcgaac ttatgcgcga ggcccagcga
     1201 cgacgccgcg ccaaactcaa agaggaagtg gggcaacgat gaatatcgac gacaccataa
     1261 ttccggatag cacccaatta aacgctgaag acatgctggg cggtccggtc actgtgacaa
     1321 taaccagtgt tgaccgcggt accgccgagc agcctgtcaa tatctacctc gaccagtttt
     1381 caggtcgtgc ctatcgcccc tgtaagtcca tgcgccgcgt gttagtcgcc gcctggggtt
     1441 ccgacgccgc caaatatata ggccgacgca tgacactgtt taatgatccg tcggtcaagt
     1501 gggctggaat ccagacaggt gggattcgta tcagccatat gagccacatc gaccacaaac
     1561 tcacgctcgc gctcaccctg acaagaggca agcgcgcacc ctttgttgtc gagcctctac
     1621 ccgacgcacc accacccatt agcgacgagg acgcggccga cttcgcgcgc tgcatcgccg
     1681 aatcgtccac cgttgcggaa ctggattcgg tcgccactgc gctcaaaagt tgcgacctcg
     1741 ggagccatcg gaagcggctg cagactgcct gggctgagcg caaggcggcg cttgaaacgg
     1801 aggcgcagtg agcatcttcg acgacctcgc acgccgcaac agccccacag tcaaggccgc
     1861 tttgcgcgaa aagtgccccg tttgcaaggc gattccgggc gaagactgca ccaatttacc
     1921 tatcaacgga tttccacttc aaaatcggct agttcactat gcgagggcta caggataatg
     1981 gctaatgcg
//
LOCUS       DASZYN010000613         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_93058, whole genome shotgun sequence.
ACCESSION   DASZYN010000613 DASZYN010000000
VERSION     DASZYN010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93058"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="VHE33_15775"
     CDS             complement(<1..1419)
                     /locus_tag="VHE33_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98965.1"
                     /translation="MRWTHLPRSDRSLTYKLSFWRRSRVAYDMIPVRIPKLAIGGLLL
                     GSALLLPYPALAQTTPLPSTGEAPPRISSAAIPSHPYSVVGPRGAILGQQDGSAEVWL
                     FPWKILDQMHLNVEMQDYPVPIDANRQASSIDVEPDQTTITYSHANFTIRQIMLAPKA
                     PADAPGALVLFQFAAVRPMTVTFSFNPVMQRMWPADSPDRPSPEWVHNPDGSGFYMLH
                     LNFPEPAAAIALPGAGPGILPPYQERASAWPLQFVLHFDPSRDSGKVYPMLFAFAGST
                     GDATTEALRQNIDRLQANVSTIRSQNAGYYRDFLARSTSIETPDAHLNAAFSWAETSI
                     DQLKVDTGSAGKQQALTAGFVGSGDAARPGFGWFFGRDALWSLYAVNSYGDFTTTKEE
                     IRFLIDHQSREGKIMHEWSQTAGMVDWAALPYQWASSDATSLFLMAASDYLRISGDRA
                     FIASIWPDLQRAWIFESTHDSDG"
     gene            complement(1500..>2220)
                     /locus_tag="VHE33_15780"
     CDS             complement(1500..>2220)
                     /locus_tag="VHE33_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98966.1"
                     /translation="SHDIDHESYQFLIDPRIGRGNSYYNRRHAFVFAGNYDLPFGRNK
                     QFAATAPGWMNQIIGGFQLNANLTLQGGIPFNTNYQLCGSQDNDIAGNDNACFLNKVS
                     GKSFGISKGSLDPIAHRVPYIKPSPYVLQPPGQANNSFGAYARPAAGTFGNIGRDALW
                     GPGFFNVDSTLAKNFTLPENVKMQFIVEAFNVFNHTNYGNPNSCIDCGDSNNAGYIQG
                     TFSEQDGTSMRRLQFAARFQF"
BASE COUNT          462 a          589 c          732 g          437 t
ORIGIN      
        1 gccatcgctg tcgtgcgtgc tctcgaaaat ccaggcacgt tgcaggtccg gccagatcga
       61 tgcaatgaac gcgcggtcgc cactgatgcg gaggtaatcg ctggcagcca tcagaaagag
      121 agaagtcgca tcggaggaag cccactgata gggcagggca gcccagtcca ccatgccggc
      181 ggtctgggac cattcgtgca tgatcttgcc ttcccgactc tgatggtcga tcaggaaacg
      241 gatttcttcc ttcgttgtgg tgaagtcgcc gtagctgttc actgcataga gtgaccacag
      301 ggcatcgcgg ccgaagaacc agccgaagcc cgggcgggcc gcgtcgccgg agcccacaaa
      361 tcctgcggtg agagcctgct gttttccggc agacccggtg tcgactttta gctgatcgat
      421 cgaggtttcg gcccacgaga aggcggcatt cagatgagca tccggcgtct cgatggatgt
      481 actgcgtgcg aggaagtcgc gatagtagcc ggcgttttgg ctgcgaatcg ttgaaacgtt
      541 tgcctgcagc cgatcgatat tctggcgcag cgcctcggtt gtcgcgtcac cggttgaacc
      601 ggcgaacgca aaaagcattg gatagacctt gccactgtcg cggctgggat cgaaatgaag
      661 aacaaactgg agaggccacg cacttgcgcg ctcctgatac ggcggcagaa tgcctggacc
      721 ggctcctggc aaagcgatgg ctgcggcagg ctccggaaag ttgaggtgca gcatataaaa
      781 gccggatcca tccgggttat gcacccactc gggagatggc cggtcgggcg aatcggcggg
      841 ccacatgcgc tgcatgaccg gattgaagct gaacgtcacc gtcatcgggc gaacagccgc
      901 gaactggaaa aggacgaggg cgccaggcgc atcggctgga gctttcggcg cgagcatgat
      961 ctgacggatg gtgaagttag cgtgagagta ggtgatggtg gtctgatcgg gttctacatc
     1021 gatgctggag gcctgccggt tcgcatcaat cgggacaggg tagtcctgca tctcgacgtt
     1081 caggtgcatc tgatcgagaa ttttccaggg gaaaagccaa acctcagcgg agccatcctg
     1141 ctggccgaga atcgcgccgc gcggtccgac gaccgaatag ggatgcgagg ggatggccgc
     1201 gctgctgatg cgcggcggag cttcgcccgt cgatggcagg ggcgttgtct gagccagagc
     1261 tggatagggc aataacagag cgctccccag cagcagcccg ccgattgcaa gcttcggtat
     1321 ccgcacagga atcatgtcgt acgcgacccg ggagcgccgc cagaaactca gtttgtacgt
     1381 cagggaacgg tcggagcggg gcaggtgggt ccagcgcatt ctatccccat acagtcttaa
     1441 ggcgccatgc gggagaagac ggcacaacga agccgccttc tcccattgcc ggcgagcgat
     1501 cagaactgga accgggcggc aaactggagc cgcctcatgg aggttccgtc ctgttcagag
     1561 aacgtgccct gaatgtagcc tgcgttgttg ctatcaccgc agtcgatgca gctgttggga
     1621 ttgccgtagt ttgtgtggtt gaaaacgttg aaggcttcaa cgatgaactg catcttcaca
     1681 ttttccggaa gggtgaagtt cttcgcgagg gtggaatcga cgttgaagaa tcccggtccc
     1741 cacaaggcat cgcggccaat attgccgaag gtgccggcag cgggacgggc ataggctccg
     1801 aaggagttat tggcctgtcc cggaggctgc aggacgtagg gcgaaggctt gatatacggc
     1861 acgcgatgag cgatcggatc gagactgccc ttggagattc cgaagctctt gccggatacc
     1921 ttgttcagga agcaggcatt gtcgttgccg gcaatgtcgt tatcctggct gccgcaaagc
     1981 tgatagttcg tgttgaacgg gatgccgccc tgcaacgtga ggttggcatt gagctggaac
     2041 ccgccaatga tctggttcat ccagcctggc gccgtcgcgg cgaactgttt gttgcgtccg
     2101 aagggcaggt cgtagttacc ggcaaagacg aacgcgtggc ggcggttgta atagctgttg
     2161 ccgcggccga ttctgggatc gatcaggaat tgataggact catgatcgat gtcgtgcgac
//
LOCUS       DASZYN010000614         3647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_286953, whole genome shotgun sequence.
ACCESSION   DASZYN010000614 DASZYN010000000
VERSION     DASZYN010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286953"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VHE33_15785"
     CDS             <1..280
                     /locus_tag="VHE33_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98967.1"
                     /translation="QRWRLERRMRMGYDGGKSTRPELGWTQRNFICAQMMIEERDFYD
                     PEKGVYTVELAPSEMEKVTRGFFAHHPQTTPKSFALWGPVAWGPVHSE"
     gene            complement(337..1776)
                     /locus_tag="VHE33_15790"
     CDS             complement(337..1776)
                     /locus_tag="VHE33_15790"
                     /inference="COORDINATES: protein motif:HMM:NF022214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HVT98968.1"
                     /translation="MLIDQTEWERTPEGLGGWLILLLLAMWISAAALLASGVSALLPL
                     FHHAPVASFPRMSPFTAALAVAAGIFGAVAGYLLIRKNPLGPTVARTLLALTAGYSLM
                     VLLATIFRVGPVPAESVPAWVEPTGYLLASVLGLGYLLRSSRVAKTYRSQPEDSNLSS
                     VSYEEDLSDVLQTRLRRWEELRSAENPPSQPSEPNLPVQRESNPETRQESTFELFQPE
                     PGHDYLQHFMPADRPIPPTTSSPEMTPPLKTLSSRNHAVPDTQELTALKTQIADGISL
                     WLTSTAAKPEVQATLFGEPIGPASAEKIRRKLLDQVNTICDQAWSVHIGDSPTLPNTP
                     ESEGSLPREIQKWAIAQAVFRLTRSLDIRAALEVYGPFEKVAEDHAFLMAVGQKNSSE
                     DAFGRKVDLAEYEGHSGPEIAYKLILRAQRDMFEADMWSRVASLAGDPDFLNRFSGGG
                     ARAYEESLEYWRGYANRMRDERVPAAHGL"
     gene            2266..>3647
                     /locus_tag="VHE33_15795"
     CDS             2266..>3647
                     /locus_tag="VHE33_15795"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98969.1"
                     /translation="MTSLFHSCLVSRRVVRQRFAKSIIPVQVLLALLGWAAAPGAQAQ
                     TIFPAAAVGTSTPTTQTVTVNVQTAGTVAGVEVLTMGAPNLDFTESGTDTCAGISSGS
                     CQVTVAFAPKYPGTRNGAVVLLDAANNTLGTQYLTGIGQGSLAVMVPGAISIAAGQVG
                     EWTLVNDGGLATQADLYLPSGIAVDGAGNLFIADSHHNRVREVFAAGANAGKIATVAG
                     DGSAGYDGTATIAKATSLNQPGGVAIDGAGNLYIADTNNNVIRKVNLTTGAISTVAGT
                     GAPGYTGDGALATVATLNSPEGVTVDAAGDLFIADTNNNAVREVVAATGRIATFAGDG
                     TGAPGFAGDSGLANGAKLNAPYGVALDSAGNLYIADSGNNRVRVVNTSGHIDTFAGNG
                     TPAYLGDGAAAISAELYSPQGVACDPAGNVYIADARNYVVRKVNAVSKVITTIAGTNA
                     NDTYSDGKSA"
BASE COUNT          735 a          973 c         1236 g          703 t
ORIGIN      
        1 gcagcgctgg cgcctggagc ggcggatgcg gatgggttac gacggcggga aatccacgcg
       61 gccggaactc ggatggacgc aacgaaattt catctgcgcg caaatgatga tcgaagagcg
      121 cgacttctac gatccggaga agggtgtgta cacggtggag ttggcgccat cagaaatgga
      181 aaaagtaacc cgcggattct tcgctcacca cccccaaacc accccaaaga gcttcgctct
      241 ttggggccct gttgcgtggg gccctgttca ctcagaatga cagcccttaa ttatgtagca
      301 aatgtttgga cacgacatca gccggacgtg gggggatcaa aggccgtggg ccgcgggcac
      361 tcgttcgtcg cgcatgcgat tggcgtagcc gcgccagtat tcaagcgact cctcgtatgc
      421 cctggcgccg ccgcctgaaa atcggttgag aaagtccgga tcgccggcga ggctggcgac
      481 gcgcgaccac atgtcggctt cgaacatatc gcgctgggct cgcaggatga gcttgtaggc
      541 gatctcgggc ccggagtggc cctcatattc ggcgagatcc actttgcggc cgaaggcatc
      601 ttcggatgaa ttcttctggc ccacggccat gaggaatgcg tgatcttcgg cgactttctc
      661 aaacgggccg tacacttcga gagcggcgcg tatgtcgaga gagcgagtga ggcgaaagac
      721 ggcctgcgca atggcccact tctgaatttc tcgcggcagc gagccttcgg actccggagt
      781 gttggggagg gtaggcgagt cgccgatgtg caccgaccag gcctggtcac aaatcgtgtt
      841 gacctgatcg agcagcttac gccgaatctt ttcggcggag gcgggcccga tgggttctcc
      901 gaacaaggtg gcctgaactt ccggtttggc agcggtggaa gtcagccaga ggctgatgcc
      961 gtcggcaatt tgcgttttga gtgcggttag ttcctgcgtg tccggcaccg catgatttcg
     1021 actggagagg gttttcaggg gaggggtcat ctctgggcta ctcgttgttg gggggatggg
     1081 ccgatctgca ggcatgaaat gctgcaggta gtcgtggcct ggttcaggtt gaaagagctc
     1141 gaacgttgat tcctggcgcg tttccgggtt cgattcccgt tgcacgggca ggttgggttc
     1201 cgatggctgc gacggagggt tttccgcaga acgcaattcc tcccagcgac ggaggcgggt
     1261 ttggagaaca tccgataagt cttcttcata ggacacggaa gagagattgg agtcctcggg
     1321 ttggcttctg taagttttcg ctacgcgact ggagcgaagg agatagccga gaccgaggac
     1381 tgaggccagg aggtaaccgg tcggctcgac ccaggcgggc acggattcgg cggggacagg
     1441 gccgactcga aagatcgttg ccagcagaac catgagggaa tagcccgcgg tgagagccag
     1501 gagagtcctt gcgacggtgg gtccgagggg gttttttcga atgagtaaat aacctgcaac
     1561 ggctccgaaa atacctgcag cgacggcaag tgcggccgtg aagggactca tcctggggaa
     1621 ggacgcaacc ggcgcgtggt ggaaaagcgg gagaagcgcc gagacgccgg atgccaggag
     1681 agcagcggcg ctgatccaca tggcgagcag gagcaggatc agccagccgc ccaggccttc
     1741 gggggtgcgc tcccattctg tctgatcgat caacacgctt tatcgtgctc cccgtttttc
     1801 tcaagaggcg cgaaaccctg gatcggatgg aagacgcctg ttcggcgagg atagacggac
     1861 gagacaaggg gcgggtatcc ctcgtcggcg cgaaacgatc cccttttggt gcttgttggg
     1921 tcgcagaagg gaagctcgtt tccggagtaa tttagcgcgc caattgccag cgcgaggtcg
     1981 cggttttctg tggaaagtac gcacctgtcg taactggttt tccctcaatg gttctttcgc
     2041 ataacacctg tgtgcaggag tagcacataa gtaccagtcg agaatcacta aggtaatttt
     2101 tccaggtgac tgtgcatgct atgttgccaa cattatcggt aacagggact gactctcctg
     2161 gcgctcaaga tgccggctct tagtcggttc gctcgccctg ctgccccctg ccgatttcgc
     2221 cttcctcgac ccgaaccggc agggacaaca aaaaggggta cctgtatgac gtctctgttt
     2281 cactcctgcc tggtttctcg tcgtgtcgtt cgtcagcgtt ttgcaaaatc aatcattcca
     2341 gtccaggtgc ttctcgcact gctgggatgg gcagccgcac caggagctca ggcgcagacg
     2401 atcttccccg cggcggcagt gggaacttcg acgcctacga cgcagacggt gacggtgaac
     2461 gtgcagacgg cggggacggt ggccggggtc gaggtgctga cgatgggtgc gcctaacctg
     2521 gactttaccg agtcagggac agacacttgc gccggaatca gctcgggcag ctgccaggta
     2581 acggttgcgt ttgcgccgaa atatccgggg acccgcaatg gagccgtggt gctgctggac
     2641 gcggcgaaca acactctcgg cacgcaatat ctgacaggaa tcgggcaagg ctcgctggct
     2701 gtgatggtgc cgggtgcgat cagcatcgcc gccggacagg tgggcgagtg gacgctggtg
     2761 aacgacggcg gtctggcgac gcaggcggat ttgtacctgc cttcaggaat tgctgtcgat
     2821 ggcgccggga acctgttcat cgcggactcg caccacaatc gggtgcgtga ggtattcgcg
     2881 gccggagcaa acgcaggaaa gatcgcaacg gttgcgggcg acggcagcgc cggatacgac
     2941 ggaacggcga cgattgcgaa ggcgacctcg ctgaaccagc cgggaggcgt tgccatcgat
     3001 ggtgcgggga atctgtacat cgcggacacg aacaacaacg tcattcggaa ggtgaacctg
     3061 acgacgggag cgatctcgac agtcgcggga accggagcac cgggttacac gggcgacggc
     3121 gctctggcaa ccgtggcaac gctcaattcg cctgaagggg tgacggtgga tgcggcgggc
     3181 gatctgttca tcgccgacac gaacaacaat gcggtgcgtg aagtcgtggc ggcaacgggc
     3241 agaattgcaa ccttcgcggg cgatggaacg ggtgcgccag ggttcgcagg cgacagcggg
     3301 ctggcgaacg gcgcgaagct gaatgccccg tatggggttg cgctggattc tgcggggaac
     3361 ctgtacattg cggattcagg caacaaccgc gtccgcgtgg tgaacacgag cgggcacatc
     3421 gacacgttcg cggggaatgg gacgccggcg tatctgggcg atggcgccgc ggcgatctct
     3481 gccgaactgt attcgcctca gggagtggcg tgcgatccgg cgggaaacgt ctatatcgcg
     3541 gacgcgcgca actatgttgt gcgcaaggtg aatgccgttt cgaaggtgat cacgacgatc
     3601 gcgggcacca acgctaacga cacctacagc gatggcaagt cggcttt
//
LOCUS       DASZYN010000615         6264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_936327, whole genome shotgun sequence.
ACCESSION   DASZYN010000615 DASZYN010000000
VERSION     DASZYN010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936327"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VHE33_15800"
     CDS             <1..691
                     /locus_tag="VHE33_15800"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HVT98970.1"
                     /translation="RRLRYTRSMSSEQGGLFGGVNPQRGGAEWVTAYCDGGSRGNPGP
                     AGYGVFIEGPGGEKLAELSEYLGKKTNNVAEYSGLLAALEWALQAGRRRLRVVADSEL
                     MVKQMQGRYKVASPDLRPLYEEAKRRVAQLEGFRIEHVLRGKNKNADRLANLAMDRGT
                     GRSGGVGSGSASGGNARPGTGRPGGAGVVAERASEPFRAAKGFVKGGVVHLIEGELPE
                     GAFVKVVLDRQ"
     gene            complement(748..1767)
                     /locus_tag="VHE33_15805"
     CDS             complement(748..1767)
                     /locus_tag="VHE33_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98971.1"
                     /translation="MKLALLFALALPLAAQTATPQPDFAAPAPSTTALQSTSQPAATP
                     SSIQPLLDFRTSDIKFNLADLTGVLRDRRHEGWVLAAYPDPKTGRPLIGAGFSLDLPE
                     REHAQTDPLNPNTFVEPSSAQLWQAAGLDPALLQHVLDQFSDNLAAWRTAHRYRRKIR
                     SLDPQITDDQAAALLRISAIQATVNARAWCRNFDQLTGPQQMAVSQLVYQMGVNLGQF
                     STFLALLNNDAVRASALADPGAASQAETSASDVQRWRTIQQSLMQSQWARLYRVRATA
                     VIAMLDPRYLDNPSASEARIAAVLRPTRHRRHSRPTLRTASYRRRSNRTLARNAPGKR
                     TRRKA"
     gene            1998..3149
                     /locus_tag="VHE33_15810"
     CDS             1998..3149
                     /locus_tag="VHE33_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98972.1"
                     /translation="MATISVENPAATATKRMLSLDVLRGLTIAFMIMVNNNGGHAWWP
                     FEHADWNGWTPTDMVFPTFLFVVGVAIVFSTESRLDRGEATGVLVGHIFRRFAILFGL
                     GLFLALFPLFHWSHLRVWGVLQRIALCYLACSLLYVGTRKASNKAVVYASLVAVLLVG
                     YWAMMRFVPVPGYGLPGRDVPFLDKDGNLVAWLDRTMHIGRLYETTRDPEGLLSTFPA
                     IGTALLGMLAALWLRTRRTLEQRCAGLLGGGAALIVLGLLWNPWFPINKKLWTSSFVL
                     FMAGCSAVIWALFMWLIEIKGCRKGWGFLLVFGMNAIASYVLSDLLVAPLWAIHMGPH
                     VSLGQWIYARSFAHIHPAGLGSLTYSIAFTLACWLVMLVFYRKRIFIKV"
     gene            3238..5415
                     /locus_tag="VHE33_15815"
     CDS             3238..5415
                     /locus_tag="VHE33_15815"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HVT98973.1"
                     /translation="MGRLLRKFEVAVFRLVRRDPPVGFVHRGAFWLFVGYLALSLPGW
                     LPGTVGRFFSDLATLDFFLLLIICVPLLWRFIFLRLLWKVRNRLIVTYLLMGLTPVVL
                     FVTLALILLYVFAGQFAIFAATSVVHDELDRIASMNRSLSMHMVHVFDENPRVRTVEV
                     PPEAIRPTQSEYKGMTVAVYLDGRQVPLTPTEWESKDPPVVPGWLHGNFTNMTFDRGR
                     LWLRAADEQVMNGHRTVVISSVPLEKENVEAIAQGLGTLSIYPGVNFAELQQEGQAAP
                     KGGFEYKVARPDQSPAHVEVNGEDITQARKRAVIAGTLPAALHFYDVPVGFLAPLTTI
                     EWSSGQETKVYTDVTSRPSLLYRRLFITSLGVGDVIREVLIVIAITFGVLELLAFLAA
                     VRLNRTITKSVHDLYEATLAIDGGNLEHRIQVKRRDQLGALSRSFNSMAASLARLLVE
                     QREKERLQNELTIAQEVQANLFPHGRIALPMLELHGVCLPARTVSGDYYDFLLFGNTG
                     LGMALGDISGKGISAALLMATLHSAVRAYRFAGEELVTEGAMAMANPRTRRVGEQEVE
                     CAELFEEPAKILSLLNRHLYRSTQPEKYATLFLAHYDGLSRRLVYSNGGHLPPLLLRA
                     NDTVTRLDCGGSVVGLLDDLEWEQATVHLGVGDILIAYSDGVTEPENDFGEFGEGQLL
                     EVVRAHRHLSLEAISEQVIRTLKSWIGAQEQPDDVTLVLARQR"
     gene            complement(5501..>6264)
                     /locus_tag="VHE33_15820"
     CDS             complement(5501..>6264)
                     /locus_tag="VHE33_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98974.1"
                     /translation="KTICYAENWIYAPAIQKETDIVTKSGAQILWILGNQSHSGSHSP
                     YYGIWRFSGGGSLVGKSCHPLSAALFLKRAEGVARDGKPIRPATVSARTHEITRLQNF
                     RNEGFLRTGYQDIEDYGQMHVVFTDGSVADIFASELVLGGVANWLEVMANNHRTQCHL
                     NPIDALTTFNPSPDNFRDVYLTEKIETKLGWSHPAPDEAWQHGYPQEFQDFVESIAAG
                     REPQSNAELARDTMATIYAAYVSAERHGAEAAIPN"
BASE COUNT         1086 a         1486 c         2413 g         1279 t
ORIGIN      
        1 ccgccggctt cgttacactc gctctatgtc ctcagagcag ggtgggttgt ttggtggagt
       61 gaatccgcag cgcggtggcg cggagtgggt gacggcgtac tgcgacgggg gatcccgggg
      121 gaatccgggg ccggcgggat acggggtctt cattgaaggg ccgggcgggg agaagctggc
      181 cgagttgagc gagtacctgg gcaagaagac gaacaatgtc gcggagtact cgggtttgct
      241 ggcggcgctg gagtgggcgc tgcaggcagg acgcaggcgg ctgcgggtgg tggcggattc
      301 ggagctgatg gtgaagcaga tgcagggccg gtacaaggtg gcgagcccgg atttgcggcc
      361 gctgtatgaa gaggcaaagc ggcgggtggc gcagctggag gggttccgga tcgagcatgt
      421 gctgcggggg aagaacaaga atgcggatcg gctggcgaat ctggcgatgg accggggtac
      481 tggccgttcg ggcggcgttg ggtcggggtc tgcgagtgga gggaatgcgc gtccagggac
      541 tggccgtcca ggcggtgctg gagttgttgc ggagcgggcc tcggaaccgt ttcgggcggc
      601 gaaggggttc gtcaagggtg gcgtggtgca cctgattgag ggggagttgc cggagggggc
      661 ttttgtgaag gtggttctcg atcggcagta ggtagccagt agccaacagc cagtagccag
      721 tagccggtag ccagtagagg agacttctta ggctttgcgc cgcgtgcgtt tccccggcgc
      781 gttcctggcg agggtgcggt tggagcggcg gcggtaggat gcggtgcgga gagtgggacg
      841 gctgtgacgg cgatggcggg tggggcgcag cacggcggcg atgcgggctt ccgaggcaga
      901 aggattgtcg aggtagcgcg ggtcgagcat ggcgatgacg gccgtggcgc gcacgcgata
      961 gaggcgagcc cactggctct gcatgaggga ttgctggatc gtgcgccagc gctgaacgtc
     1021 ggaggctgag gtttctgcct gcgatgccgc gccgggatcg gccagggctg aggcgcgcac
     1081 ggcatcgttg ttgaggagtg cgaggaacgt cgagaactgg ccgaggttca cgcccatttg
     1141 atagacaagc tggctgacag ccatttgttg agggccggtg agctggtcga agttgcggca
     1201 ccaggcgcgg gcgttgacgg tggcctggat agcggaaatg cgcagaaggg cagcggcctg
     1261 gtcgtcggtg atctggggat cgagggagcg gattttgcga cggtaacggt gcgcggtgcg
     1321 ccaggcggcg agattgtcgc tgaactgatc gagcacatgc tgcaggagcg cggggtcgag
     1381 cccggcggct tgccatagct gggccgacga gggctcgacg aaggtgttcg ggttgagcgg
     1441 gtcagtttgg gcgtgctcgc gttcagggag atcgaggctg aagccagcgc caatgagggg
     1501 gcggccggtt ttgggatcgg gatacgcggc gaggacccaa ccttcgtgtc ggcggtcgcg
     1561 gagcacgccg gtgagatcgg cgaggttgaa tttaatgtcg gaggtccgga aatcgagaag
     1621 aggctggatc gacgacgggg tcgctgcagg ctgactcgtc gattgcaggg ctgtcgttga
     1681 aggcgcaggg gcagcgaaat ccggctgtgg cgttgcggtt tgtgctgcga gagggagtgc
     1741 aagcgcgaac agcagcgcca gcttcaaggt ttggagcgct ggacgaccgg gtcgggatgg
     1801 agctttccta cgcacgagta cggtacacga tgacggaaac agcgagctga agcaatcaca
     1861 aaaaaggatc accttcgaga accatcggca gtaacgttcc gttcgtgacg gctgggggag
     1921 aaacttttta gagcgaggct ggcgggaagt gtacagaagc cgcgattcca ggtacggtgt
     1981 tgaggttgag gagaaccatg gcaacgatct ccgttgaaaa tccggccgcg acggcaacga
     2041 agcggatgct gtcgctggat gtgctgcgcg ggctgacgat tgcgttcatg atcatggtga
     2101 acaacaacgg cgggcacgcg tggtggccgt tcgagcatgc ggactggaac gggtggacgc
     2161 cgaccgacat ggtgttcccg acgttcctgt ttgtggtggg ggtggccatc gtgttctcga
     2221 ccgagtcacg gctggatcgg ggcgaagcga cgggcgtgct ggtgggacat atcttccgcc
     2281 gattcgcgat cctgtttggg ctggggttgt tcctggcgct gttcccgctg tttcactggt
     2341 cgcatctgcg ggtgtggggc gtgctgcaac ggatcgcctt gtgctacctg gcgtgcagcc
     2401 tgctgtatgt ggggacgcgg aaggcttcca acaaggcagt ggtgtacgcg agcctggtag
     2461 ccgtgctgct ggtgggctat tgggcgatga tgcggtttgt cccggtgccc gggtacggcc
     2521 tgccggggcg ggatgttcca ttcctcgaca aggacgggaa cctggtggca tggctggacc
     2581 ggacgatgca cattgggagg ctctatgaga cgacgcgcga tccggagggg ctgctgagca
     2641 cgtttccggc aattgggacg gcgttgctgg ggatgctggc ggctctgtgg ctgcgaacgc
     2701 ggcggacgct ggagcagcga tgcgcagggc tgctgggtgg gggagcggca ctgatcgtgc
     2761 tggggctgct gtggaatccc tggttcccga tcaacaagaa gttgtggacg agctcgttcg
     2821 tgctgttcat ggcggggtgc tcggcggtga tctgggcgct gttcatgtgg ctgatcgaga
     2881 tcaaaggctg ccgcaaggga tgggggttcc tgctggtgtt tgggatgaac gcgatcgcca
     2941 gctatgtgtt gtcggatttg ctggtcgcgc cgttgtgggc gattcacatg ggacctcacg
     3001 tgagtctggg gcaatggatc tacgcgcgaa gctttgcgca tatccacccg gcggggctgg
     3061 ggtcgctgac gtattcgatc gcattcacgc tggcgtgctg gttggtgatg ctggtgttct
     3121 atcggaagag gatctttatt aaggtctagc cagtagccaa taggcaacag gcaaacggtc
     3181 gctggctgct tttcttcgtc tcgtcgttgc gccgggaatg caatagactg gcgtcacatg
     3241 ggaaggctgc tgcgcaaatt tgaagttgcg gttttccgcc tggtgcgccg cgacccaccg
     3301 gtcggctttg tgcaccgcgg ggctttctgg ctgtttgtcg gctatctggc gctgtctctg
     3361 cctgggtggc tgcctgggac ggtggggaga ttcttcagcg atctggcgac gctggacttc
     3421 ttcctgctgc tgatcatctg cgtgccgctg ctgtggcggt tcatcttcct gcgactgctg
     3481 tggaaggtgc gcaaccggct gatcgtgacg tatctgctga tgggactaac gcctgtagtt
     3541 ctttttgtga cgctggcgct gatcctgctg tatgtgtttg cggggcagtt cgcgattttc
     3601 gcagcgacga gtgtggtgca cgatgagctg gaccgcattg catcgatgaa tcgcagcctg
     3661 agcatgcaca tggtgcatgt attcgatgag aatccacgag tgcggacagt ggaagtgccg
     3721 ccggaggcga ttcgtccaac gcaaagtgaa tataagggga tgacggtcgc ggtgtatctg
     3781 gacggcaggc aggttcccct gacaccgacc gaatgggaat cgaaggatcc gccggtggtg
     3841 ccggggtggc tgcacgggaa ctttacgaac atgacgttcg accgcggacg gttatggctg
     3901 cgcgccgccg atgagcaggt gatgaacggg catcggacgg tggtgatctc cagcgttcct
     3961 ctggagaaag agaatgtgga ggcgatcgcg caggggctgg gcaccttgtc gatttacccg
     4021 ggagtgaatt tcgcagagct gcaacaggaa gggcaggctg cgccgaaggg ggggttcgag
     4081 tacaaggttg cgcggcccga tcagtctcct gcacatgtgg aagtgaacgg agaggacatc
     4141 acgcaggcgc ggaagcgagc tgtgattgcc gggaccctgc cggcagcatt gcacttttac
     4201 gatgtgccgg tgggattttt ggcgccgctg accaccattg aatggtcgag cggacaggaa
     4261 accaaagtct atacagacgt gacgtcgcgg ccgtcgctgc tctatcggcg cctgtttatc
     4321 acctctcttg gggttggcga tgtgattcgc gaggtgctga tcgtgatcgc gatcacgttc
     4381 ggggttctgg aattgctggc atttttggcg gcggtgcggc tgaatcggac gattacgaaa
     4441 tcggtgcacg atttatacga ggcgacgctg gcgatagatg gcgggaatct tgaacaccga
     4501 atccaggtaa agcggcgcga tcagctgggg gcgctgagcc ggtcgttcaa cagcatggcc
     4561 gcgtcgctgg cgcggctgct ggtggagcag cgcgaaaagg agcggctgca gaacgaactg
     4621 acgattgccc aggaagtgca ggcgaatctg tttccgcatg ggcgaatcgc gttgccgatg
     4681 ctggaactgc acggggtttg tttgccggcg cggacggtga gcggcgatta ctacgacttt
     4741 ttgctgttcg gaaatacggg cctgggcatg gcgctggggg atatcagcgg gaagggaatt
     4801 tccgcggcgc tgctgatggc gaccctgcat tctgcggtgc gggcttatcg atttgccggc
     4861 gaggagctgg ttacggaagg cgcgatggcg atggcgaatc cgcgaacccg gcgggtgggc
     4921 gagcaggaag tggagtgcgc cgagttattt gaagagcctg caaagattct gtcgctgctg
     4981 aaccggcatt tgtatcgcag cacgcagccg gagaagtacg caacgctgtt cctggcgcac
     5041 tatgacgggt tgagccggag gctggtgtat tcgaatggcg gacatttgcc accgttactg
     5101 ttacgtgcca acgatacggt gacgcggctg gactgcggcg gttcggtggt ggggctgctg
     5161 gatgacctgg agtgggagca ggccacggtg cacctgggcg tgggcgacat tctgattgct
     5221 tacagcgacg gggtgacgga gccggagaac gatttcgggg aattcgggga agggcagctg
     5281 ctggaagtgg tgcgggcgca tcgtcacctg agtctggagg ccatctccga gcaggtgatc
     5341 cggacgctga agagctggat cggggcacag gagcagccgg acgacgtgac gctggtgctg
     5401 gcgcggcaga gataaagcag cgtacagcgt tcagttgtca gcgttcagga ggcgctcggc
     5461 gatccccgtt cggcgttccg acagaggcca gcctggagga tcagttaggg atcgctgctt
     5521 cggcgccgtg gcgttcggcg gagacgtagg ccgcgtagat ggtggccatg gtgtcgcggg
     5581 cgagttcggc attggattgt ggctcgcgac cggcggcgat gctctcgacg aaatcctgaa
     5641 actcctgcgg gtagccgtgc tgccaggctt catcgggagc gggatggctc cagccgagtt
     5701 tggtttcgat cttttcggtg aggtaaacgt cgcggaagtt gtcgggcgag gggttgaagg
     5761 tggtgagcgc gtcgatcggg ttgaggtggc attgggtgcg gtggttgttg gccatgacct
     5821 cgagccagtt ggcgacaccg ccgaggacga gttctgaagc gaagatgtcg gcgacagagc
     5881 cgtcggtgaa gacgacgtgc atctggccgt agtcttcgat gtcctgataa ccagtgcgca
     5941 ggaagccctc gttgcgaaag ttctggagac gggtgatttc gtgggtgcga gcgctgaccg
     6001 tggcaggacg gatgggcttg ccgtcgcgcg cgacgccttc ggcgcgtttg aggaagagtg
     6061 cggcgctcaa ggggtggcag cttttgccga cgagggaacc gccgccggaa aagcgccaga
     6121 tgccgtagta gggggaatgc gagccggagt gagactggtt gccgaggatc cagaggattt
     6181 gtgcgccgga tttcgtaaca atgtcggttt ctttttggat ggccggggcg tagatccagt
     6241 tctcggcata acaaatggtc ttac
//
LOCUS       DASZYN010000616         6336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_861042, whole genome shotgun sequence.
ACCESSION   DASZYN010000616 DASZYN010000000
VERSION     DASZYN010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6336
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861042"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(469..1212)
                     /locus_tag="VHE33_15825"
     CDS             complement(469..1212)
                     /locus_tag="VHE33_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98975.1"
                     /translation="MALPVQDKTGIAIPVTLAHGPVVDHLIVAAQLGYSTLGDGTKVM
                     PVDFYTAILADPDDPTDPSGFGFWVVILNALPTPLSLVEMPALVHGYQKGYPAVSDLK
                     GSTLTTHVIPAARSHPLDPSVGLCGVGTWAFDFGNGTEGAMALCYNGNLTDPQSQQAV
                     GPFVGISWRIGLAWANRFAVTADVAGKYGTLDGFYQQSVVSASDSIRYDKSSEIVVMC
                     HEWSADLQSGIDPLCSSMMVYIRNKLPNE"
     gene            complement(1223..2821)
                     /locus_tag="VHE33_15830"
     CDS             complement(1223..2821)
                     /locus_tag="VHE33_15830"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HVT98976.1"
                     /translation="MPDSEKLMGMAANTAILGAFACEAPELAFVIGAAQFFLDMVWPA
                     AQAPTQMSPVDDAYLSSALDDLKSEITDAMWLLQLKVHTDAVLHASSIYWGYLKQFQQ
                     MTIDTDPESEDELDAYFRTAKSKEYPFDVLDNARLYITTDATVDKDLTPLQVAEHYTK
                     TIGLYAVIGSAMIAYLKASVAWRRAKLMYPYLEYAKYQVALAKYEKLDPNHQHPAWKP
                     ADVPDPNPNGKNPLPDWHDWIQREESGVPRLIETVNTLVGYAEGTPATATAPATTGLQ
                     VDMAQHWKATIDNIRARLDQMWVAPQIPRGNYDGSGKDLGPFWVVDTAVGRPYVEYTV
                     AAGDTLTSIASTQSRKTYSRLTADDLFFMNFDVFVDYGRMTSGGGYQIAQASWWQLDK
                     TYDDATFLLPGMVLKLPNLHTRPPMSREFAGCQWFCRSGAALAYEWETSMDHFALSGI
                     NERDITDFGKVIQLWKRARASVQFTPYTVKAGDTLLSIAGGDAVLADKIYKFNSDTLK
                     SPAQNLIAGQVINIPDASVLSDIQ"
     gene            complement(2855..>6336)
                     /locus_tag="VHE33_15835"
     CDS             complement(2855..>6336)
                     /locus_tag="VHE33_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98977.1"
                     /translation="NVDPDKIARAIFAGFQSELQAQDAGLIELQFPVGRGAQGLLFGP
                     VQDDLTQAAPPPGRAPKPVRAPIANMFPGPKTDPRSGDDDDDGDGVELRPVASQASSN
                     SALGRVVVDARAGQSGQQAITNENSQFQAAVAGGNPPQNYDVLPANPNKSAAQPGIYE
                     GMDQPLDGPSQPEQGTATTPTTQSQTTVVPKDQPGASNIDDDASESASQNDQQGCSIC
                     CKAVIAISAVAIVLGTVGTLLWAYVFGGATKKSDSGTGTTGSGPGKLDTSDQTVTKKD
                     LDATVDVDLPSLNKLMMVDNITFSTPTFTPPPAQGSGGSWSMTPSIHALFTPDKGQVG
                     AFSSISVQYVLEATIQGSKVKSDARTLTVNFTPSPAVDINVTANDRTSKLPIDMSMYY
                     SNFAGTIKIQKSTGGTWVNDADPKIVNYTPDAMTGTTATAQYERVTTWGAASANSATI
                     TVTVPAVVNPPQPTGTAEFLPNDAKTQGDWLHVYGADGYLIAGDKTSNPSYVTPTLST
                     QTVSSLSSTTTGIPLVKASDPTKNIAAAWSATSPVTIDLACSDSTTVHQVALYFADYD
                     PKAGGQTVDALDAKGTKLATQTLPATLTGGLYLVCNISGHVQLQISNAAGNATLSGIF
                     FGGPVVSAAFAKSDTKSQGTWPGVYGADGYTIAGDQTKNPSYVTPSIYAASSGPPQTP
                     FVQAASTSEVRALEKSSKPADHIAGTWYADARGGSDSLLIDLPFSDTAVHQVAIYCLD
                     WDNSGIVQTVDVLDPSGFVLDTQKLSSFGNGIWLVWKMMGHVHIRIRNSAGDNGTVSG
                     VFFDPPPAMNMTLAAFSAPPAAWDFTVWMTDAPQTVKVDFLKRCQIPAGFKSIEFANG
                     TSDVTVPKQGRWSLESTGTPPAVVFQPEVGLALTQGYSMKYRVVDQNGLKSNEATMTV
                     DYAPLARMMPRAGNFLSSADPRYGKFAAAEVLTGCSAAFGIAKGTVKLMGLQDILQGN
                     PQPQAYLRNDSKSMCVVGEGIWMVDDNDLIVFQSDPNLSYPPTPARFCFTDSKGYQSN
                     VAVVVFDSSLTDALTQVPQALGKMTDADFWKNYAANVIPASSDPPPEQFIAITQMLAV
                     ITRTFGSVGPEPDYDFNTNYDQWSGGSWADLQTICAKMVASAIGNGAPQFAARYWQLN
                     LMVRMALKAYPPS"
BASE COUNT         1198 a         1761 c         2021 g         1356 t
ORIGIN      
        1 agcgacccag gatgagcccc gagggcgctc tccgagccac ccgtcccggc gatctgaatc
       61 atgatgatct cgatgggtac ccaccatggc ttgccgagtg gttgaaggca tgcttgcccg
      121 atgacatgga tgcacagaag cggatgctgc tagctgttca ggacaccgcg atccaattac
      181 gcatttggca caggtaagat ccgggtggga gccggtgtcc agaattcggt tcaggaaagc
      241 catgactttg gaagccatat cgcgcctggt accgagaacc atgaggagac gggcttcttt
      301 ttcctcgtgg acggatgtaa ctgggaccgt cccctcacca gcgacagaat atcgggaaga
      361 ttgttgagac ggagggatgg acacactgat cgatattcgc cggcgtctcc gatatggcgg
      421 tccttatgca cagattgaag tacaacggaa cacaacctgg accgccacct attcgttggg
      481 taacttgttg cgaatgtaaa ccatcatcga gctgcagagt ggatcgatcc cggactgcag
      541 atcggccgac cattcgtggc acatcaccac gatttcgctg ctcttgtcgt aacggatact
      601 gtcggaggcg gaaacgacgc tctgctgata gaagccgtcc agggtgccgt actttccggc
      661 tacgtccgca gtgaccgcaa atcgattggc ccaggccagg ccgattcgcc acgaaatacc
      721 gacgaatggg cctacggcct gctgggactg agggtcggtc aggttgccgt tgtagcagag
      781 ggccatcgca ccttcggttc cattcccaaa gtcgaaggcc cacgtcccga cgccgcagag
      841 accgacgctg gggtcgagag gatgagagcg ggcagcgggt atcacgtgcg tggtcagcgt
      901 ggaccctttc aggtcggaga ccgcagggta gcccttttgg tatccgtgaa ccagggcagg
      961 catttcgacc aggctcagcg gcgttggaag ggcattcagg attaccaccc aaaagccaaa
     1021 gccgcttgga tcggtcggat catcgggatc cgccaggatc gccgtataga aatcgaccgg
     1081 catgaccttt gtgccatccc ccagagtgga gtagcccagc tgagcggcga caatcagatg
     1141 atcgacgacc ggtccatggg cgagggttac gggaatcgct atgcctgtct tgtcctgaac
     1201 gggaagggcc acgtgcgtct ccttactgga tgtcggaaag gaccgatgca tcagggatgt
     1261 tgatgacctg gccagcgatc agattttggg cagggctctt cagagtgtcg ctgttaaatt
     1321 tgtagatttt gtccgccaga actgcgtctc cgccggcgat gctgagcagc gtatcgccag
     1381 cctttacggt gtacggcgtg aactgcacgc tggccctggc cctcttccat aactggatca
     1441 ctttgccgaa gtcggtgatg tcgcgctcgt tgattcccga caatgcaaaa tggtccatcg
     1501 aggtctccca ctcgtaagcc agcgcagcac ccgatcggca aaaccactgg caaccggcaa
     1561 attcgcgtga catgggggga cgcgtgtgca ggttagggag cttgagaacc atccctggca
     1621 ggaggaaggt agcgtcatcg taggtcttgt caagctgcca ccagcttgcc tgggctatct
     1681 ggtagcctcc gccgctggtc atccggccgt agtctacgaa cacgtcgaag ttcatgaaga
     1741 acagatcgtc ggctgtgagc cgagagtacg tcttacgcga ttgagtggag gcaatactgg
     1801 tgagcgtgtc gccggcggct actgtgtatt ccacataggg ccgacccacc gcggtatcga
     1861 cgacccaaaa gggtccgaga tcctttccgg aaccatcgta gtttcctctt ggaatctggg
     1921 gagccaccca catctgatcg agacgcgcgc gtatgttgtc gatggtggcc ttccagtgct
     1981 gtgccatgtc gacctggagg ccggtggtgg ccggcgctgt cgcagtggcc ggcgttccct
     2041 ctgcataccc gactaaagtg ttcaccgttt cgatcagcct ggggacgccg gactcctctc
     2101 tctggatcca gtcgtgccaa tcgggaagcg gattctttcc gttcgggttg ggatcgggca
     2161 catccgccgg tttccaggcg gggtgttggt ggtttgggtc cagcttttca tacttagcca
     2221 gagcgacctg atacttcgca tactccaggt agggatacat caacttcgcg cgccgccagg
     2281 ccaccgacgc cttcaggtac gcaatcatgg cgctgccgat caccgcatac agtccgatag
     2341 ttttggtgta atgctcggcc acctggagag gcgtcagatc cttgtcgacg gtggcatccg
     2401 tggtgatgta gagcctggca ttgtcgagaa catcgaaggg atattccttc gactttgctg
     2461 tacgaaaata ggcgtcgagt tcatcctccg attccggatc ggtatcgatc gtcatctgct
     2521 ggaactgctt caggtatccc caatagatgc tgctggcatg caggaccgca tccgtatgta
     2581 ccttcagttg cagcagccac atcgcgtccg tgatttcgct cttcaggtcg tccagcgccg
     2641 aactgaggta ggcatcgtcg acgggagaca tctgggtggg cgcctgtgcc gcgggccaga
     2701 ccatgtcgag gaagaactgg gccgctccaa tcacgaacgc caactcgggc gcttcacagg
     2761 cgaacgcgcc gaggatggcg gtgttggccg ccattcccat cagtttttcc gaatcaggca
     2821 tacggcactt cctttccgga ctggtgagaa acaattacga gggcggataa gccttcagcg
     2881 ccatgcggac catcagattg agctgccagt agcgagcggc aaactgaggc gcgccgttgc
     2941 caatcgcgga ggcgaccatc ttcgcgcata tcgtttgcag gtcagcccaa ctgccgccgc
     3001 tccactggtc gtagttggtg ttgaagtcat agtccggctc ggggccgacg cttccgaagg
     3061 tgcgggtgat gaccgccagc atctgcgtga tggcgatgaa ctgctcgggc ggcggatcgg
     3121 acgaggccgg tatcacgttc gccgcgtagt tcttccagaa atcagcgtcg gtcatcttgc
     3181 caagggcctg ggggacctgg gtcagggcgt cggtcaggct ggaatcgaac acgaccaccg
     3241 cgacgttgga ctggtagccc ttgctgtcgg tgaaacagaa gcgggctggg gtgggtgggt
     3301 aggagaggtt gggatcgctc tgaaagacga tcagatcgtt gtcgtccacc atccagatac
     3361 cttcgccgac aacgcacatc gatttgctgt cgttgcgcag gtaggcctgg ggttggggat
     3421 tgccctggag aatgtcctgc aatcccatca gcttcacggt tccttttgcg attccgaagg
     3481 cggccgagca tcctgtgagg acttcggcag cggcgaattt gccgtagcga gggtcggcag
     3541 aagagaggaa attaccggct cggggcatca tgcgggccaa cggggcgtag tccaccgtca
     3601 tggttgcttc gttggacttg agaccgttct gatcgacgac gcggtatttc atcgagtagc
     3661 cctgcgtcag ggccaaaccc acttcgggct ggaacacgac ggcaggcggc gttccggtgg
     3721 attcaaggga ccagcggccc tgcttcggca ccgtgacgtc gctggtgccg ttggcgaact
     3781 cgatggattt gaaaccagcc ggaatctggc agcgcttcag gaagtccacc ttgacggtct
     3841 gcggcgcatc ggtcatccag acggtaaaat cccatgccgc gggtggggcg gagaaggctg
     3901 ccaatgtcat gttcatggcc ggtggaggat caaagaagac gccgctcacc gttccgttat
     3961 ctccggcgct gttgcgaatg cggatatgca catggcccat cattttccat acgagccaga
     4021 tgccgttgcc gaaggacgag agcttctggg tgtccagaac aaaaccgctg gggtcgagca
     4081 cgtcgacggt ctgaacgatt ccggagttgt cccagtccag gcagtagatg gcgacctggt
     4141 ggacggcagt gtcggagaag ggcaggtcga tcagcagcga gtccgaaccg cctctggcgt
     4201 cggcatacca ggttcctgcg atatgatcgg ccggcttcga gctcttctcg agcgcgcgga
     4261 cttcgctggt cgatgcagcc tggacaaagg gagtttgcgg cggaccggag gaggcggcgt
     4321 aaatcgaggg cgtgacgtag ctggggtttt tggtctgatc gccggcgatc gtgtagccgt
     4381 cagcgccgta gacacccggc caggttccct ggcttttggt atcagacttg gcgaaggcgg
     4441 cgctcaccac cggtccaccg aagaaaatgc cgcttaaagt ggcgttcccc gccgcattcg
     4501 atatctgcaa ttgaacatgg ccgctgatgt tgcatacgag atagaggccg ccggtcaggg
     4561 ttgctggcag cgtctgtgtc gccagctttg tgcctttggc gtccagagcg tcgacggttt
     4621 gcccgccggc cttcggatcg taatcggcga aatagagtgc gacctgatgg actgttgtgc
     4681 tgtcgctgca ggccagatcg atcgtgactg gtgaagtggc ggaccaggcg gccgcaatgt
     4741 tttttgtcgg atcggaggcc tttaccagcg ggatgccggt cgttgtgctg ctgagagaag
     4801 acacggtttg ggtagagagg gtgggcgtga cgtagctggg attcgacgtc ttgtcgccgg
     4861 cgatcaggta gccgtcagcg ccatagacgt ggagccagtc gccctgcgtc ttcgcatcgt
     4921 tgggcaggaa ttcggcggtg cccgtgggtt ggggcggatt gacgaccgcg ggcaccgtga
     4981 ccgtgatggt ggcggagttg gcggaggccg cgccccaggt cgtgaccctt tcatattgag
     5041 ccgtggccgt tgtcccggtc atggcgtctg gcgtgtaatt gacgatctta gggtcggcgt
     5101 cgttgaccca ggttcccccg gtcgacttct gtatcttgat cgtgccggca aaattgctgt
     5161 agtacatcga catatcgatg gggagcttgc tggtccggtc gtttgccgtt acgttgatgt
     5221 cgactgccgg gctcggagtg aagttgacgg tcagcgttct cgcatcggac ttgaccttgc
     5281 ttccctggat cgtggcctcg agaacatatt gaacagagat cgaggagaag gcgcccacct
     5341 gccctttgtc aggggtaaac agagcgtgga tcgacggcgt catgctccag ctgcctcccg
     5401 aaccctgagc gggcggcggc gtgaacgttg gcgtggagaa ggtgatgtta tcgaccatca
     5461 tcagtttatt gagcgatgga agatcgacgt ccacagtcgc atcgaggtct tttttggtca
     5521 ccgtctgatc gctggtatcc agcttgcctg ggcctgaacc ggttgtcccc gtgccggaat
     5581 cggacttctt cgtggctccg ccgaatacgt aggcccacaa caatgtccca acggtaccga
     5641 ggacaatcgc aacggcagag atggcaatca cagccttaca gcagatgctg caaccctgct
     5701 gatcgttctg gctggctgac tcggaggcgt catcatcgat gttgctggcg ccaggttgat
     5761 cctttgggac taccgtggtc tgcgactgcg tcgtcggcgt cgttgcggtt ccctgctcgg
     5821 gttgggaagg gccatcgagg ggctgatcca ttccttcgta aatgcccggt tgagcagccg
     5881 atttgttcgg atttgccggc aggacgtcgt agttctgcgg cggattgcca ccagccaccg
     5941 ccgcctggaa ctggctgttc tcgttcgtga ttgcctgctg gccactttga ccggcccggg
     6001 cgtcgacaac tactcgcccc agcgccgaat tggaactggc ctggctcgcg acggggcgca
     6061 attcaacacc gtctccatcg tcgtcgtcat cgcctgatct gggatcggtc ttcggccccg
     6121 gaaacatgtt ggcgatgggc gcgcggactg gtttgggcgc ccggcctggc gggggagccg
     6181 cctgggtcag atcgtcctgc acgggaccga agagcaatcc ctgcgcgcct cgcccaacgg
     6241 ggaactgcag ttcgatcagg cctgcatcct gagcctgtaa ctcggactgg aatccggcaa
     6301 agatcgcccg cgcgatcttg tcagggtcca cattcg
//
LOCUS       DASZYN010000617         1893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_226135, whole genome shotgun sequence.
ACCESSION   DASZYN010000617 DASZYN010000000
VERSION     DASZYN010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226135"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            382..1395
                     /locus_tag="VHE33_15840"
     CDS             382..1395
                     /locus_tag="VHE33_15840"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HVT98978.1"
                     /translation="MNEHPTPEHILQTGLGFWPSKVLLSAIEMGVFTELAHGPEPLET
                     LRGRLGLHVRSAHDFLDTLVALGFLERRNGTYANTPATDLFLDRKKPSYMGGILEMAN
                     HRLYPFWGHLTEALRTGNPQNEARTGDTPTFEALYADPARLKEFLAAMSGISRGAAMA
                     IAAKFPWSKYKTFCDVGAAQGDTASQIALAHSHLNGLGFDLPEVGPIFEEYVEALGLS
                     QRLRFEPGSFFDSPLPKADVIVMGHILHDWDLETKRMLIRKAWEALPEGGAYIVYEAI
                     IDDDRSKNAFGLMMSLNMLIETPGGFDYTGADCQGWMRAAGFRESRVEHLVGPDSMVI
                     GIK"
     gene            complement(1510..>1893)
                     /locus_tag="VHE33_15845"
     CDS             complement(1510..>1893)
                     /locus_tag="VHE33_15845"
                     /inference="COORDINATES: protein motif:HMM:NF028447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5110 domain-containing protein"
                     /protein_id="HVT98979.1"
                     /translation="DRIPLYVRAGSIVPMGPEIEYANENPDGPIELRIYRGADASFDF
                     YEDSGDGYGYEKGQHAVIPLHWNDATGELTIGAREGSYPAMPAARQFHVVLVGKAHGV
                     GEAVSAQADDDVSYGGREVKVKVRQ"
BASE COUNT          362 a          690 c          469 g          372 t
ORIGIN      
        1 gccacctctg cttccgcttg tcgttccgca accgctcagt ccaagagtca acaacgacag
       61 cgagaaacaa aatagaaaaa tgtgcaaaaa cttgtcgcgc aacggtctct ccagactcct
      121 gttgaagtca gccagcttgg atgcgcaact tccgctttgc gttttccgtg ctcaacttcc
      181 ctttccctaa gccattgcaa attcattggc ctcaataccg ttgaatccgg cataatggca
      241 cggcaccccc acgcagcggc ctgtcatctc ttcgcacctc ctcggctgac agcctccatc
      301 cccggttcct gatctgtccc ctcggcgggc ctgcgcagga ggtgcgcctt gcgttacatc
      361 catgcaatca gggagtttct tatgaacgag catccgaccc cggaacacat cctccagacc
      421 ggccttggct tctggccctc caaagttctg ctcagcgcga ttgaaatggg cgtcttcacc
      481 gagctggccc acggtcccga acccctcgaa acgctgcgcg gccgcctcgg actccacgtc
      541 cgctccgccc acgatttcct cgacacgctc gtggccctcg gcttccttga gcgccggaac
      601 ggcacatacg ccaacacgcc cgctaccgat ctcttcctcg atcgcaaaaa gccttcctac
      661 atgggcggca tcctcgagat ggccaaccac cgcctgtacc cgttctgggg acacctcacc
      721 gaagccctgc gcaccggcaa cccgcagaac gaggcgcgga ccggcgatac tccaacgttt
      781 gaagccctct acgccgaccc cgcccgtctc aaggaattcc tcgccgcgat gtcgggcatc
      841 agccgcggcg ccgccatggc catcgccgca aaatttccct ggtcgaagta caaaaccttc
      901 tgcgacgtgg gcgccgcgca gggcgacact gcctctcaga tcgcccttgc ccattctcat
      961 ctcaacgggc tcggctttga tctgcccgaa gtcggtccca tcttcgagga atacgtcgaa
     1021 gccctcggcc tttcgcagcg tctccgcttc gaacccggga gcttcttcga ctctccattg
     1081 cccaaagccg acgtcattgt catgggccac atcctccacg actgggacct cgaaaccaag
     1141 cgcatgctca tccgcaaagc gtgggaagcc ctgcccgagg gcggcgctta cattgtctac
     1201 gaagccatca tcgacgacga ccgcagcaaa aacgccttcg gcctcatgat gagcctcaac
     1261 atgctcattg aaacccccgg cggattcgac tacaccggcg ccgactgcca gggctggatg
     1321 cgcgctgccg gcttccgcga aagccgggtc gaacacctgg tcggcccgga ttccatggtc
     1381 atcggcatca agtaatcggc atcaagtaac aacagtcagc gaacaagcct ctgggtgccc
     1441 cacgtccctc gagttgggac gtggcgcacc gacgcgagcg cgccgctttt ccgaacgctg
     1501 atcactgcct cactgccgta ccttcacctt cacttctcga ccgccgtagc tcacatcgtc
     1561 gtcagcctgc gccgacaccg cttctcccac gccatgcgct tttcccacca gcacaacgtg
     1621 gaattgccgg gccgcaggca tcgccggata cgatccctcc cgtgccccga tcgtcagttc
     1681 gccggtcgcg tcgttccagt gcagcgggat cactgcgtgt tgccctttct cgtacccgta
     1741 gccgtctccg gagtcctcat agaagtcgaa gctggcgtcg gcgccgcggt aaatcctcag
     1801 ttcgatcgga ccatccggat tctcgttggc gtactcgatc tccggaccca tcggcacaat
     1861 cgatcccgcc cgtacgtaca gcggaatccg atc
//
LOCUS       DASZYN010000618         3067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_352820, whole genome shotgun sequence.
ACCESSION   DASZYN010000618 DASZYN010000000
VERSION     DASZYN010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352820"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VHE33_15850"
     CDS             complement(<1..1175)
                     /locus_tag="VHE33_15850"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HVT98980.1"
                     /translation="MIRATLQRIRAELQRFYLLSRGHLWFPHLPLAILLVSGGLWMLH
                     ARFASEWRQYVNQLMHGEIHLSPLLLPPLLIGGGMVMMGLALLWRSRLAWTIALLLAA
                     LGDVNTALTGHTHVHALLAYFSFTVLALALAWRRFDRTSLAASTLFALTSVLMLLLYA
                     TFGSYYLGKEFKPPIHDLITALYYAMVTMSTVGYGDIVPTTSESRLFALSVMILGVAV
                     FATSLTAVIAPLVGRSMQRIMNRSGRRMKRENHFVVVGNTPLAVNTWRELARRNRPVT
                     RLLREMPEEGQVKDVDLVVGDPSMTDVLREAGAHKAEAVLAMLADDSENAFCVLAVRE
                     LAGKARTVAAVNDARHLGRVRLAQPDVVIAPQVLGGELTAMLLSGEEVTSDFVMQRV"
     gene            complement(1172..2014)
                     /locus_tag="VHE33_15855"
     CDS             complement(1172..2014)
                     /locus_tag="VHE33_15855"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HVT98981.1"
                     /translation="MHPLLFHFGHIGVPTYGVCMALALLAALAVSVVAAKRAGLGAEK
                     VWNLDLIVILTALFAGRLLMIMMHPHLFRSHPFWLLGLLTLPNVWFALLGALAGFLAG
                     GLYVLAEGLPLLKTADVLAPAVAIAFCLSRIGSFCAGSAWGTPTHLPWGVVYRSPVAY
                     LWYRVPLGVTLHPVQIYDAMASLGLFGLLMWAGWRRGSRRDGEIAGAWLFLYGVIRFF
                     LEFLRGDPGREPLLGGALTLAQLLSIGAVVMGGLLWLRSGPAFVAPQAVPAEIADPGR
                     ADHQ"
     gene            complement(2088..2278)
                     /gene="ssrS"
                     /locus_tag="VHE33_15860"
     ncRNA           complement(2088..2278)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VHE33_15860"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(2430..>3067)
                     /locus_tag="VHE33_15865"
     CDS             complement(2430..>3067)
                     /locus_tag="VHE33_15865"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVT98982.1"
                     /translation="DAATGKELWRFKTGEDPQYHNQVGIQSSATIADGVVYFGCRDSN
                     LYALDAATGNLRWKFNNKGSWVITSPVVRNKKIYFATSDTALLHVLNAADGTPIDSLH
                     FFWPIFASPSIAGDTLYLGGQDGKLVAVDLAAWKVAWTFQTEGSKKNLAPLSKPDGKP
                     NYEAVFASPFYDDMIIGVSKTHTVGTINASPVISGDTVYVGSADGNLYALH"
BASE COUNT          672 a          984 c          916 g          495 t
ORIGIN      
        1 aagacgcgct gcatgacgaa atcggaggtc acttcttcgc cggagagcag catcgccgtc
       61 agctctccgc ccagcacctg cggtgcgatc accacgtcgg gttgtgccag ccttacccgg
      121 cccagatggc gtgcatcgtt caccgctgca acagtgcgcg ccttgcccgc cagttcgcga
      181 accgcgagca cgcagaaggc gttctcggaa tcgtcggcga gcatcgccag aaccgcttca
      241 gctttgtggg ctcccgcttc gcgcaacaca tcggtcatgc tgggatcgcc caccaccagg
      301 tcaacatcct tcacctggcc ctcctcaggc atctcgcgca aaagccgtgt gactgggcgg
      361 ttgcgccgtg ccaactcgcg ccaggtgttg acggcgagtg gggtgtttcc gacaacgaca
      421 aaatgatttt cgcgcttcat acgcctcccg ctgcggttca tgatgcgttg catgctgcgc
      481 cccaccagcg gcgcgatgac cgcggtgagc gaagtggcga aaaccgcgac gcccaggatc
      541 atgaccgaaa gcgcgaacag gcgcgattca ctcgtggtgg gcacgatatc gccgtaaccg
      601 acggtgctca tggtcaccat tgcgtagtag agcgccgtga tcagatcgtg aatgggcggc
      661 ttgaattctt tgccgagata atagctgcca aacgtggcgt acagcagcag catgaggaca
      721 gaggtcagcg cgaacagcgt gctcgccgcc agactcgtcc ggtcgaagcg ccgccaggca
      781 agagcaaggg ccagaactgt gaacgaaaaa taggccagca gtgcgtgaac atgcgtgtgc
      841 ccggtgagtg ccgtgttcac atcccccagg gcggccagca gcagcgcgat cgtccacgcc
      901 agccgtgaac gccacagcag cgccagcccc atcatgacca tgccgccgcc aatcagcagc
      961 ggaggcagca gtaagggact caggtggatc tcgccgtgca tcaactggtt gacgtactgc
     1021 cgccactccg atgcgaagcg cgcatgcagc atccacaggc cgccagagac gagcaggatg
     1081 gcaagaggaa gatgcggaaa ccaaagatgc ccgcgggaaa gaagatagaa ccgctgcagc
     1141 tcggctcgaa tgcgctgaag cgtggcgcgg atcattggtg atccgctctc cccggatccg
     1201 caatttctgc cggaaccgcc tgtggagcga caaacgccgg accagagcgc agccacaaca
     1261 acccgcccat caccaccgcg ccgatggaga gcagctgcgc cagcgtcaaa gcaccgccga
     1321 gcagaggctc gcggcccggg tcgccacgca gaaactccag aaaaaagcgg atcacaccat
     1381 aaagaaagag ccaggctccg gcaatttctc catcgcgccg gctcccccgc cgccagcccg
     1441 cccacatcag caacccaaag agcccgagcg aggccatcgc atcgtagatc tgcaccggat
     1501 ggagcgtcac cccgagcggc acgcggtacc agagataagc caccgggcta cgatagacca
     1561 ccccccaggg aagatgcgtc ggcgtccccc acgcagatcc agcgcagaac gatccaatgc
     1621 ggctcaggca gaatgcaatt gccaccgcag gagccagaac gtctgcagtt ttcagcagcg
     1681 gcaatccttc agccagcaca taaagcccac cggccaaaaa gccggccagc gcgccgagca
     1741 gagcaaacca gacattcggc agggtaagaa gccccagcag ccagaacgga tggctgcgaa
     1801 acagatgcgg gtgcatcatg atcatcagca gacgccccgc aaacagtgcg gtgagaatga
     1861 caatcaggtc gagattccag accttctccg cgcccaatcc tgctcgcttc gccgccacca
     1921 cggacactgc aagcgccgcc agcagcgcca gcgccatgca gacaccatag gtgggtacac
     1981 cgatatgccc gaaatggaag agaagagggt gcacgcgcag atcagcgtgg actgagaacg
     2041 aggacaaagc ccgaacaagc gggttttggc ccgctgagga agtaaaagac cgacccgctg
     2101 ggccgtgggg cgatgcgctg gtgaacccgt cctaagacgg tgggaactct ccgcggttct
     2161 ttaggcattc cggactggcg gacactgcac accgaaccga tttgtcgagt cgagctccct
     2221 gttcaatgaa tgtaggttca tccagcgttg accgccctca cctgctttgc aggccggtct
     2281 cttgagttgg atgctaacgg catctcctgc tgattggcaa gccaccgaat gtggacttct
     2341 tcctcggtag ctccaaagtg ggattccgct ccgttacggt tctgccgcga ttcccccgaa
     2401 gacagcggct gtacggcgcc acaaaccaac tagtggagcg cgtacagatt gccgtcggcg
     2461 ctccccacat agaccgtgtc gccggagatc accggcgacg cattgatcgt ccccaccgta
     2521 tgcgttttgc tgacgccgat gatcatgtcg tcgtagaaag gggaggcgaa gacagcctcg
     2581 tagttgggct tgccgtcggg tttcgacagc ggcgccaggt ttttcttcga gccctcggtc
     2641 tggaacgtcc acgcgacctt ccaggccgcc agatcgaccg ccaccagctt gccgtcctgc
     2701 ccgccgagat agagcgtgtc acccgcgatc gagggggagg cgaagatcgg ccagaagaaa
     2761 tgcagcgagt cgatcggggt gccgtccgcc gcgttcagca cgtggagcag ggccgtgtcg
     2821 gaggtcgcaa agtagatttt cttgttccgc acgaccggcg acgtgatgac ccacgagccc
     2881 ttgttattga acttccagcg cagattcccc gtcgctgcgt cgagcgcgta gaggttggag
     2941 tcgcggcaac cgaagtagac cacgccatcg gcgatcgtcg ccgaggactg gatgccgacc
     3001 tggttgtgat actgcgggtc ctcaccggtt ttgaatcgcc acagttcctt tcccgtcgcc
     3061 gcgtcca
//
LOCUS       DASZYN010000619         4071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_226169, whole genome shotgun sequence.
ACCESSION   DASZYN010000619 DASZYN010000000
VERSION     DASZYN010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226169"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /locus_tag="VHE33_15870"
     CDS             <1..1437
                     /locus_tag="VHE33_15870"
                     /inference="COORDINATES: protein motif:HMM:NF037850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HVT98983.1"
                     /translation="DVIGYNYMDPGALAYHQAHPDKPVIGTETVSAVGTRGIYITDPA
                     KGYLGSYDPYTTTGRASAEGWWRFCEANPWLAGGFVWTGFDYRGEPSPYQWPNISSQY
                     GIIDTCGFPKDTYYYYQSWWTDRPVLHLFPHWNWPGYEGKEIAVWVHSNLDRVELFVN
                     GRSLGAQEVKKNQHVAWNVPYAAGAIEARGYKADKVVMTTRRETTGPAAKLVMTADRQ
                     EANADGEDVVMFAVAVHDAQGREVPIADNEVAFQISGPGKLIGVGNGDPTDHAPDKGT
                     SRKAFSGLCMAIVQSTKKPGSITVQATSPGLVPATVTISTRKVSLRPQVAVWEREVPK
                     GSGVTGLWRPAPEAGAANEMVALFVGNDSTLFTLRQSGNSLTGSVEGGGGGFFSGNEL
                     PVPIEDAKIDGSSISFKAGTSTYTGTISGDRIKLDRKIQLPFRRGQPVEATGPRPAIG
                     PPPDGSDPSFNRRRMPDSIPVVLQRVER"
     gene            1544..2908
                     /locus_tag="VHE33_15875"
     CDS             1544..2908
                     /locus_tag="VHE33_15875"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HVT98984.1"
                     /translation="MAPPPEDGEISHNTKLTPKLGLFDSTMMVMGGIVGAGVFMNPYV
                     VAQRLHSPVLILSVWVAGGIIAILGSFIYAELGARIPEVGGQYEYLRQSLHPVFGFLY
                     GWALLLVIQTGGMAAVTVTFARYFLELTGMPVSEHRVVVVTLALLTGVNCLGVKPGGR
                     FQSILMVLKIAAIAALVGAGLFLVRAPKPMLHPILDRPLSFDLLTALGAAMVPVLFSY
                     GGWQTTNFIAGEIRDARRNLGRALLIGVAAVVVLYISVNFVCLRTLGVTDLAATSVPA
                     SSVMQIVAGQRGARLIALGIAVSTIGFLSQSVLTGPRVYFAMAEDGLFFRAVAYVTRK
                     TQVPVVAIVLQSVWAAVIALSGKYEQILNFQAPIDFTFFGLSATCLFVLRRRERSQPT
                     SDPGFRVPGHPYTTLIFVLSCWLIVANVLWKYPRNSSIGFVIVLIGLPVYALWRRRPR
                     EASE"
     gene            2905..4002
                     /locus_tag="VHE33_15880"
     CDS             2905..4002
                     /locus_tag="VHE33_15880"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HVT98985.1"
                     /translation="MTDARAVSSTYLEWAKLCSRAKYNLATSGIMSYPLAELHISAQN
                     LEINGPDAYGYVPLVERLARKCGVAPNCVVTAAGTSMANHLAMAALIQPGDDVLIEQP
                     SYGPILDVASYLGANILRCERRAEHDFQVDPSDVARHITPKTRLICLTNLHNPSGAML
                     SDETLTRIGQIAEKSGAQVLVDEVYLDMVYDQPVRSSFHLGGNFVVTNSLTKAYGVSG
                     LRCGWILAQPGLAKRMWRLNDLFAATPVHPGEMLSVEALDQLDKIDARARAILDTNRK
                     ALDAFFSGRSDLNGFRSSWGTVTFPRLKNGRVDEFCEFLRTRYETSVVPGRFFELADY
                     FRIGIGGDTAMTAEALKRLAAALDEWGTNRA"
BASE COUNT          754 a         1378 c         1145 g          794 t
ORIGIN      
        1 gatgtcatcg gctacaacta catggatccc ggcgcgctcg cctatcacca ggcccatccg
       61 gataagcccg tcatcggcac cgaaaccgtc agtgccgtcg ggacgcgcgg gatctacatt
      121 accgatcccg ccaaaggcta ccttggttcc tacgatcctt acaccaccac cggccgcgcg
      181 tcggcagaag gctggtggcg tttctgcgag gccaacccct ggctcgccgg tggctttgtg
      241 tggacaggtt tcgattatcg cggtgagccc tcgccttatc agtggccgaa catcagctcg
      301 cagtacggca tcatcgatac ctgcggattt ccgaaagaca cttactacta ctaccagtcc
      361 tggtggaccg atcggcccgt gctccatctc ttcccccact ggaactggcc cgggtacgaa
      421 ggcaaagaga tcgctgtctg ggtccattcc aatctcgatc gcgtcgagct cttcgtgaat
      481 ggccgcagcc tcggcgccca ggaagtaaag aaaaaccagc atgtcgcctg gaacgtcccg
      541 tatgctgccg gagccattga ggcgcgcggc tacaaggcag acaaagtcgt gatgaccacc
      601 cgccgcgaaa ccaccggccc agcggcaaaa cttgtcatga ccgccgaccg ccaggaggcg
      661 aacgccgacg gagaagacgt cgtcatgttt gccgtcgccg tccacgatgc ccagggccgt
      721 gaggtgccca tcgcggataa cgaagtcgcc ttccagattt ccggtcccgg caagctcatc
      781 ggtgttggca acggcgaccc caccgatcac gcaccagaca aaggaacttc ccgcaaggcc
      841 ttctccggcc tctgcatggc cattgtgcag tccacaaaga aacccgggag catcaccgtc
      901 caggccacct caccaggact tgtgccggcc acggttacaa tttccacgcg gaaagtctcg
      961 ctgcgccctc aggtcgctgt ctgggagcgc gaagtcccca aaggctcggg agtcaccggg
     1021 ctctggcgtc cggcgccgga ggcaggggcc gccaacgaga tggttgccct cttcgtcggc
     1081 aacgattcca ccctcttcac cctgcgccaa agcggcaaca gccttacggg aagcgttgaa
     1141 ggcggtggcg gcggattttt cagcggtaac gaactcccgg ttcccatcga agacgccaag
     1201 attgacggca gcagtatttc cttcaaagcg ggcaccagca cctataccgg caccatcagc
     1261 ggcgatcgca tcaagctcga ccgcaagatc cagctaccgt tccgcagagg tcagcccgtc
     1321 gaagcgacag ggccgcggcc ggcgattggc ccgcctcccg acggatcgga tccttcgttc
     1381 aaccgcaggc gcatgcccga cagcatcccg gtcgtgctgc aacgcgtgga gcgctgaagg
     1441 cgaaaccgcg ggccatgggc acacaaccag agaactcgaa tagcgccggg caaaatcgca
     1501 gtccgtcgga ggcagcgcca cctctcagat agaattcgct tcgatggccc caccgcctga
     1561 ggacggcgaa atttcccaca acacgaaact cacgccgaag ctcggcctct tcgattccac
     1621 catgatggtc atgggcggca tcgtcggagc cggtgtcttc atgaacccct atgttgtcgc
     1681 ccagcgcttg cactctcccg tcctgatcct gtctgtctgg gttgccggcg gcatcatcgc
     1741 gatcctcggc tccttcatct atgcggagct cggagcccgc attccagagg tcggcggcca
     1801 atacgaatat ctccgccagt cgctccatcc cgttttcgga tttctttacg gatgggctct
     1861 cctcctcgtt attcagaccg gtggcatggc cgctgtcacc gtcacctttg cccgctactt
     1921 cctcgaactc accggcatgc ccgtttccga gcaccgcgtc gttgtcgtca ccctggccct
     1981 gctcaccggc gtgaactgcc tgggagtcaa acccggcggt cgattccaga gcattctgat
     2041 ggtgcttaag attgccgcca ttgccgctct cgtgggcgcc ggactctttc tggttcgcgc
     2101 gcccaagccg atgctgcacc caatcctcga tcgtcccctc agtttcgacc tgctgacggc
     2161 gctgggtgcg gcgatggtcc ccgtgctctt ttcctacggc ggctggcaga ccaccaactt
     2221 catcgccggc gagattcgcg acgcgcgcag gaatctcggc cgcgcgctgc tgatcggagt
     2281 cgcggccgtg gtcgttctct acatctcagt caacttcgtc tgcctccgca ccctgggagt
     2341 tacggacctg gccgccacca gcgttcccgc cagttctgtg atgcagatcg tcgccggcca
     2401 gcgtggagcg cgtctcatcg ccctcggcat tgcagtttcg accatcgggt ttctcagtca
     2461 gtccgttctc accgggccgc gcgtctattt cgccatggcc gaagacggcc tcttttttcg
     2521 cgcggtcgct tacgtcaccc gcaaaaccca ggtgcctgtc gttgccattg tgctgcaaag
     2581 cgtctgggca gccgtgatcg cgctcagcgg aaaatacgag caaatcctga atttccaggc
     2641 gccgattgat ttcactttct tcggcctcag cgcgacctgc ctgtttgtcc tgcgtcggcg
     2701 cgaacggagc cagcccacca gcgatcccgg ttttcgcgtt cctggtcatc cgtataccac
     2761 tctcatcttt gtcctgtcgt gctggctgat cgtcgccaac gtgctttgga agtatcctcg
     2821 caacagctcc atcggatttg tcatcgtcct gatcggatta ccggtgtacg cgctctggcg
     2881 gcgccggccc agggaggcat cagaatgacg gacgcgcgcg ccgtgtcatc cacctatctc
     2941 gaatgggcga agctctgctc gcgtgccaaa tacaacctgg ccaccagcgg gatcatgagc
     3001 tacccgctcg ccgagctgca catttctgcg cagaatctcg aaatcaacgg gcccgacgcc
     3061 tatggctatg tgccgctcgt ggaacgcctg gcgcgcaagt gcggcgtcgc tccgaactgc
     3121 gtcgttaccg ccgcgggcac gtcgatggcg aatcatctcg ccatggccgc tctcatccag
     3181 ccgggcgacg atgtgctgat tgagcagccg agttacggac ccatcctcga cgtagcctcc
     3241 tatctgggcg ccaacatttt gcgctgcgaa cgccgcgccg agcatgactt tcaggtggat
     3301 cccagcgacg ttgcgcggca catcactcca aagacgcgct tgatctgtct aaccaacttg
     3361 cacaatccct ccggcgccat gctcagcgac gaaactctga cccggatcgg tcagatcgcc
     3421 gagaagtccg gtgcacaggt gctggtcgac gaggtttatc tcgacatggt gtacgaccag
     3481 ccagtgcgct cgtcattcca cctcggcggc aacttcgtcg tcaccaacag cctgaccaag
     3541 gcctacggcg tcagcggcct gcgttgcggc tggatcctgg cgcaacccgg ccttgccaag
     3601 cgcatgtgga ggctcaacga cctcttcgcg gccacacccg tgcaccctgg cgagatgctg
     3661 agcgtagagg ccctcgacca gctcgacaag atcgatgcgc gggcccgcgc aattctcgac
     3721 accaaccgca aagccctcga cgccttcttc agcggtcgca gcgatctcaa cggtttccgc
     3781 agttcctggg gcacagtcac atttccgcgc ctgaagaacg gccgcgtcga cgagttctgc
     3841 gaattcctgc gcacccgcta cgaaaccagc gtggttccgg gacgtttttt cgaattggcg
     3901 gactatttcc gcatcggcat cggcggagac accgccatga cggccgaggc attgaaacgt
     3961 ctggccgccg cgctggacga atggggaacg aaccgcgcat gaatcaggcg cctcgcgtac
     4021 gcatcggcat cgactggggc ggaaccaaaa ttgaagcact cgcgatggac g
//
LOCUS       DASZYN010000620        11521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_801064, whole genome shotgun sequence.
ACCESSION   DASZYN010000620 DASZYN010000000
VERSION     DASZYN010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11521
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801064"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /gene="kdgD"
                     /locus_tag="VHE33_15885"
     CDS             <1..1143
                     /gene="kdgD"
                     /locus_tag="VHE33_15885"
                     /EC_number="4.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF002958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047448 - 5-dehydro-4-deoxyglucarate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxyglucarate dehydratase"
                     /protein_id="HVT98986.1"
                     /translation="DVVLRMSANEKWWNLHECDCFLIVLPHKGHAQISSLYRDAVRTT
                     GFRSANFQLLSSIVGRQPHQTPGWRGRFNSMSRAAMTPQQMAQRVGEGLLSFPVTHFN
                     EQYNFAEQPYRQHLAWLLDHCPAALFAAGGTGEFFSLAHDEFMRVVSAAVSEVAGKVP
                     VIAGCGCGTRAAIEFAQAGQKAGADGILLFPPYLVHAEPAGLVAHAKAVCDSTRLGVI
                     FYNRDNAILDESALEELCQSCPNLVGFKDGVGDLELMTRIHARLGDRLVYIGGLPTAE
                     TFALPYLELGVTTYSSAIFNFLPKFAQDFYAAVRRRDHAAVLSGLRDFVLPYISIRRR
                     RKGYAVSIVKAGLKAVGRDAGPVRPPLVELSAGETDELKKLIAGRN"
     gene            1152..2486
                     /locus_tag="VHE33_15890"
     CDS             1152..2486
                     /locus_tag="VHE33_15890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT98987.1"
                     /translation="MPPRNTTHVRYVIVLVLFIASTFSYGDRVVLSIAADALSHDLRL
                     DPVHLGYLFSGFGWAYALAQLPAGGLLDRFGSKSVYGISIFCWSLCAVLAGFTGYLPA
                     IAAFYTLFALRLVDGLAQAPVFPGNGRIVAAWFPTSERGRASALFNAAQYFSLVFFAP
                     IMGWIVHLWGWKQCFWFAGAVGLVLGAFWYRFIYGVKSHPRISQPEIDLIEQGGGLAS
                     LDATGRARANTLTWSAVRWLLSKRMLVGIYIGQYCITTLTWFFLTWFPVYLSRARHIP
                     IVKVGFLAALPALCGFIGGILGGVASDELLRTGRSLSFARKLPIVAGMLLAMSMVACN
                     YVSTRTAVIVIMSLAFFGKGFGALGWTVISDTSPKTLVGINGGLFNLVGNLAGVTTPI
                     VIGYIVGRTGSFRGALIFVATTALMAIVAYLPVVGEIKRLETPLGLVPGGAP"
     gene            2483..3829
                     /locus_tag="VHE33_15895"
     CDS             2483..3829
                     /locus_tag="VHE33_15895"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HVT98988.1"
                     /translation="MIPEISECDTPRVTSMRVIPVAGYDSMLLNLSGAHAPWFTRNLV
                     ILEDSAGHKGAGEVPGGESIRDVLERSQPLLLDASVGAFRQTLDSIGNCFADRDNAGR
                     GLQTFDQRTTIHAVTAIESALLDLLGQFLELPVAALLGEGQPRLSVDVLGYLFFIGDR
                     GKTDLPYREESSSSDPWFRLRNEEVLTIPALLRLTDAAEQRYGFRDFKLKGGVLPGSF
                     EMEAAAALAGHFPQARITLDPNGAWSLDEAIALCKDRRDVLAYAEDPCGAEQGYSGRE
                     IMAEFRRATGIPTATNMVATDWRQLHHAARLQAIDILLADPHFWTMRGSVRASQFCRD
                     HNLTWGSHSNSHFDISLAMFTHVAAAAAGNVTAIDTHWIWQDGQRLTREPLRIAGGSV
                     ALSDRPGLGVDLDMEQIERANQLWQRIGVSGRDDAAAMQFLIPDWSFDPKRPCLVR"
     gene            complement(3881..3956)
                     /locus_tag="VHE33_15900"
     tRNA            complement(3881..3956)
                     /locus_tag="VHE33_15900"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3921..3923),aa:Cys,seq:gca)
     gene            complement(4034..4693)
                     /locus_tag="VHE33_15905"
     CDS             complement(4034..4693)
                     /locus_tag="VHE33_15905"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HVT98989.1"
                     /translation="MTLRINDIAPDFTAETTQGTIHFHEWIGDNWAVLFSHPKDFTPV
                     CTTELGAVAGLEKEFAKRGAKVIGLSVDPVASHKKWEPDIHDVTGHQVNYPMIGDPEL
                     KIAKLYDMLPGDAGETSEGRTPANNACVRTVFVIGPDKRIKLHLAYPMTTGRNFDEIL
                     RVLDSMQLTAKHKVATPANWKQGEDVIITGAVTNEEADKVFPGYKTVKPYLRTTSQPQ
                     S"
     gene            complement(4929..6179)
                     /locus_tag="VHE33_15910"
     CDS             complement(4929..6179)
                     /locus_tag="VHE33_15910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HVT98990.1"
                     /translation="MGPIVAAVRCPVDPDKSTSIQSAGQATRPSGTDANHLFANNVRF
                     VSMAAIIAAHTIDTCSGASTTWLLQALKFGTIGFFLVAGFLFGERIDDYSSLQYFSRR
                     LRSVALPWTIWYGFYSSLSLTADFAHRRVTFHSADLVSRVYAEFSKGLLDTAYWFVPN
                     LIIALAVLLVFRRRLRDYRMGLAFLLLSLFYAANIYGGWIPVKHTRAVFGFVFYLWLG
                     AWGAWHFKAIEKWLSHIPMALMIALILVTNVLALGEARLLVSRHSEDALNTLRITNQL
                     ASIVVVLGIVKLKRAVWPRFVDVRAHTYGLYLTHMVALVLFRFVIVRALPHFISVDLV
                     NSSRAIFVLLPLTFVAVYGGSLFIVKFMLSHKRLRWAVGVARSNRPADASRSRESHRS
                     VLSSVRVGPRAKRELASLAESLTR"
     gene            complement(6373..6888)
                     /locus_tag="VHE33_15915"
     CDS             complement(6373..6888)
                     /locus_tag="VHE33_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98991.1"
                     /translation="MKLPSAVLFLPVLSFLCIASCPAQQTAMLANAPLPNVASDAQPG
                     DDFSGSAMGVFPVEAPQVQEKKPRLNLLDWSLLGATATLRVLDFTTTEKALTEPQYYH
                     EAMLPNGLVKNKPAFAAFQAGTVALNFEAYRYLVRHNHRPLARVAQFAYVGVMSMQIA
                     HNYQVLGQNPR"
     gene            complement(7580..9181)
                     /locus_tag="VHE33_15920"
     CDS             complement(7580..9181)
                     /locus_tag="VHE33_15920"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HVT98992.1"
                     /translation="MPDPPVPPSRPEGSQPRQIFARKSIDKLITESERPEHALKKTLG
                     PISLTALGIGAVIGSGIFTVIGTAMAGQKFDSPEISNTPLVHYLLTHTAMAGRPGAGP
                     ALAVSLVLVAIVCVFTGLCYAELASMIPIAGSAYTYTYATMGELIAWIIGWDLILEYA
                     FSNMSVSVGFAAHIEALVEWFGIHPALRWISPAYLPTGLQDFAGNNLYNPGWHFGFNI
                     PAFLIVMILTVVLVRGIRESARTNNILVLVKIVAILAFIFAAASFVKPHYWHPFMPNG
                     WTGMLSGGSIIFFTYIGFDSVSTAAEECRNPQRDLPFGILATLVACTLLYGGVAIVLS
                     GIVPWQSIMGDAAPVVNALKRLSVAPGGHALRWVELIVLIGAMLGMISSILVFQLGQA
                     RVWFAMSRDRLLPNIFSHIHPQFRTPAVATWVAGFVVGIPAGLLDIGTVSNLSNIGTL
                     FAFVLVSIAVLILRYREPERPRAFRAPLGPVFPVLSIIFCIVLMMGLEVLTWTAFFIW
                     LFIGLGIYFSYSRHRSEFGSRSRLG"
     gene            complement(9481..>11521)
                     /locus_tag="VHE33_15925"
     CDS             complement(9481..>11521)
                     /locus_tag="VHE33_15925"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HVT98993.1"
                     /translation="IDEYQDTNRPQYELMKLLAGEEKNVCVVGDEDQSIYSWRGADIR
                     NILEFEKDFPGAKTVRLEQNYRSTQIILEAASAVVARNAQRKGKRLWTSRQGGALIGY
                     YEAPDGENEALFVADSIQKYLRKAGEEGGDGGKAAVLYRTNSQSRLIEEAMRRYNIPY
                     TMVGGFSFYERAEIKDLLSYLKLVANPHDSIALQRVVNTPARGIGKTTMETLERIALE
                     TGSSIWTGIDRALEKQLLPQRSLQALDGFRRIITDARAMVGAGFVEKLTADVAPQATT
                     EFAAVDDTSFDFGAQGMLALTHDADPGLAADDYISESAEDFEPAKFSVFAEEAPVEHA
                     QAQNSELRAQGSEDEAVPGFRKPGGAATLPELIKFVIDRTGYIKSLEEEGTPEAFSRI
                     ENLKELANAAQDAHERGETLAEFLDHAALVSDVDQYDPEARVTLMTLHAAKGLEFPLV
                     VLAGMEEGLFPHSRTLNDPPQMEEERRLCYVGLTRAMDSLVVTRARYRRRYGNDMPEA
                     SIASRFLEEIPEHLLEDLSGGRTRSTGWGGYAAQYSQTPSRFGRSSDDEFGSRHYSYE
                     DEDQSAAFRTERPGQAARNERPRDSVDNIAKFFTGRGPAGRSVPARSSRPKLDVPEPT
                     GAKGFRSGQRVRHPKYGEGIVFRREGDGDDAKITVQFSGFGVKKLVEKFAQLERL"
BASE COUNT         2250 a         3653 c         3200 g         2418 t
ORIGIN      
        1 gacgttgtcc ttcggatgag cgcgaacgaa aagtggtgga atcttcatga atgcgattgc
       61 ttcctgattg ttctacctca caaggggcat gcgcaaatct cctcgctcta ccgggatgcg
      121 gtacgaacca ccgggttccg gtctgctaat ttccagctgc tttcctctat agttggaagg
      181 cagccgcacc agaccccagg ctggcgcgga cgttttaaca gcatgtccag agcagccatg
      241 acaccgcagc agatggccca gcgcgttggc gaaggcctac tctcctttcc tgtcacccat
      301 ttcaacgagc aatacaactt tgctgagcag ccctaccgcc agcatctggc gtggctcctt
      361 gaccattgtc cagccgccct gttcgccgct ggtggcaccg gcgaattctt ctcccttgcg
      421 cacgacgaat ttatgcgcgt ggtttcggcg gccgtctccg aggtcgctgg caaagtgccg
      481 gtaatcgccg gatgcgggtg cggaacccgc gccgcgatcg agttcgctca agccggccaa
      541 aaagcgggcg ccgacggaat cctgctcttc ccgccctatc tcgtccatgc cgaacccgcc
      601 ggccttgtcg cccacgccaa agctgtctgc gactccacac gtctcggcgt tattttctac
      661 aaccgcgaca acgctatcct cgacgaatcc gccctggaag agctctgcca gtcctgcccc
      721 aacctggtcg gattcaaaga tggggtcggc gaccttgagt tgatgacccg tattcatgca
      781 cgtctcggcg atcgtctcgt ctacatcggt ggcctgccta ctgcagagac atttgcgctt
      841 ccctacctgg aactcggggt cactacctac tcttcggcga tcttcaactt cctcccgaaa
      901 ttcgcgcagg acttttacgc cgccgttcgt cgccgcgacc acgcagccgt cttatccggc
      961 ctgcgtgact tcgttctgcc gtatatctcc attcgacggc gccgcaaagg ctacgccgta
     1021 tccatcgtca aggcaggcct aaaagcggtc ggccgtgacg ctggtcctgt tcgtccaccg
     1081 ctcgtcgaac tcagtgccgg cgagacggac gagctcaaaa agctcatcgc gggacgcaac
     1141 tgaccaggcg tatgccgccc aggaacacca cgcacgtccg gtacgttatc gtgctggtgc
     1201 tcttcattgc cagcacattc agctacggcg atcgtgtcgt gctctccatc gccgccgacg
     1261 ctctcagcca cgacctgcgt ctcgatcccg tccatctcgg ctacctcttc tccggttttg
     1321 gctgggccta cgctctcgca caacttcccg ccggcggact cctcgaccgc ttcggctcga
     1381 aaagcgtcta tggcatcagc atcttttgct ggtccctctg tgcggttctc gccggattca
     1441 ccggctatct ccctgccatc gccgcgttct acactctctt cgcactgcgt ctcgtcgacg
     1501 gtctcgccca ggctcctgtc tttcccggta acggacgcat cgttgccgcc tggtttccca
     1561 cttctgaacg aggccgcgcc tcggcgctct tcaatgcggc acagtatttt tcgcttgtct
     1621 tcttcgcgcc catcatggga tggatcgttc atctttgggg ttggaaacag tgtttttggt
     1681 ttgccggcgc cgtcggtctc gtgctcggcg cgttctggta tcgctttatc tatggcgtga
     1741 aatcgcatcc ccgcatcagc cagcctgaga tcgatctcat cgagcagggt ggaggtctcg
     1801 cctcgctcga cgctaccggg cgcgcacgcg ccaacacact tacctggtcc gcagtgcgct
     1861 ggctactcag caagcgcatg ctggtcggca tctacatcgg ccaatactgc atcaccacgc
     1921 taacctggtt cttcctcacc tggtttcctg tctatctcag ccgcgcgcgg cacatcccca
     1981 tcgtgaaggt aggatttctc gctgcgctcc ccgcactttg cggtttcatc ggcggcattc
     2041 ttggcggcgt ggcttccgac gaactcctcc gcacggggcg cagtctcagc ttcgcgcgca
     2101 aactccccat cgtcgccggc atgctgctgg ccatgtcgat ggtcgcgtgc aattacgtca
     2161 gcactcggac cgccgtcatt gtcatcatgt cgctcgcctt tttcggcaag ggcttcggcg
     2221 cgctcgggtg gaccgtcatc tctgatacgt cgccaaagac actggttgga atcaatggcg
     2281 gcctcttcaa tctcgtcggc aacctagccg gcgtcaccac gcccatcgtc atcggctata
     2341 tcgtcggccg cactggatct tttcgcggcg ctctgatctt cgtcgcaacc acggctctga
     2401 tggccatcgt cgcttacctg cccgttgttg gcgagatcaa acgactcgaa acgccactcg
     2461 gtctcgtgcc cggaggcgcc ccttgatccc ggaaatctca gagtgtgata cgccacgcgt
     2521 cacctcgatg cgcgttatcc ccgttgccgg atacgacagc atgcttctca acctttccgg
     2581 cgcccacgct ccctggttca cccgcaacct cgtcatcctc gaagatagcg ccgggcacaa
     2641 gggcgccggc gaagtcccgg gcggcgaatc aatccgcgat gttcttgagc ggtcccaacc
     2701 cctgcttctc gacgcgtcgg tcggcgcctt caggcaaacc ctcgattcca tcggcaactg
     2761 ttttgcggat cgcgacaacg ctgggcgcgg cctccagacc ttcgatcaac gcaccaccat
     2821 ccacgctgtt actgcgatcg aatcggcgct cctggatctc ctcgggcaat ttcttgagct
     2881 tccggtcgcc gcgctccttg gggaagggca gccgcgtctc tccgtggatg ttctcggcta
     2941 tctcttcttc atcggcgacc gcggcaaaac tgacctgcca tatcgcgagg aaagttcttc
     3001 ctcggatccc tggtttcgcc tccgtaatga ggaagtgctg accatccccg cgctccttcg
     3061 cctcacagat gccgccgagc agcgctacgg cttccgcgac ttcaaactga aaggcggcgt
     3121 actccccggt tcctttgaga tggaggccgc ggccgcactc gccgggcact ttccacaggc
     3181 tcgtatcaca ctcgacccca acggcgcctg gtcgctcgac gaagcgatcg cgctctgcaa
     3241 agaccgtcgc gacgttctcg cttacgccga agacccatgc ggggccgaac aaggctactc
     3301 gggtcgggaa atcatggctg agtttcgccg cgctacgggc attcctaccg ccactaatat
     3361 ggtcgctacc gactggcgcc agcttcatca tgccgcccgg cttcaggcca tcgatatcct
     3421 gcttgccgat ccgcatttct ggaccatgcg gggctccgtg cgcgcttcac aattctgtcg
     3481 cgatcacaac ctcacgtggg gctcgcattc caatagtcac ttcgacatat ccctggccat
     3541 gttcacgcac gtcgctgcag cagccgccgg aaatgtcacc gccatcgata cgcattggat
     3601 ctggcaggac gggcaaaggc tcacccgcga accccttcgc atcgccggcg ggtcggttgc
     3661 gctttctgac cgccccggcc tcggcgttga tctggatatg gagcagatcg agcgggccaa
     3721 tcagctttgg cagcgaatcg gcgtctctgg ccgcgatgat gccgcagcga tgcagttcct
     3781 tatcccggat tggagcttcg atccgaagag gccctgcctt gtgcgctaat gcgccgcgcg
     3841 agtggccgac tgagttattc gtatttccga ggcaggatac tggagcaccg ggcgggaatt
     3901 gaacccgcga atactggttt tgcagaccag cgcgttagcc acttcgccac cggtgctcat
     3961 ttcgccgcgc ttgcccggcg cggcgttgcg tcacatgtct gacagcgtgc gacgccgtcg
     4021 gacgcgcgct gatttacgac tgcggctgcg aggtcgtccg caggtatggc ttcaccgtct
     4081 tgtagccggg gaacacctta tccgcttcct catttgtcac cgcgccggtg atgatcacgt
     4141 cctccccctg cttccagttc gccggcgtcg ccactttgtg cttcgcggtc agctgcatcg
     4201 aatccagtac ccgcagaatc tcgtcgaagt tacgccccgt cgtcatcgga taggccaggt
     4261 gcagtttgat ccgcttgtcc ggcccgatca caaacaccgt gcgcacgcag gcgttgttcg
     4321 ccggtgtgcg gccttccgag gtctccccgg cgtctcctgg cagcatgtcg tacagctttg
     4381 ctatcttgag ctccggatcc ccgatcattg gataattcac ctgatgcccg gtgacgtcgt
     4441 ggatatccgg ctcccacttc ttgtggctgg caactggatc gacgctcagt ccgatcactt
     4501 tcgccccgcg cttcgcaaac tctttctcca gcccggcgac cgcgcccaat tcggtcgtgc
     4561 acactggagt gaagtccttg gggtgcgaga acagcacggc ccagttgtcg ccgatccact
     4621 catgaaaatg gatcgtgccc tgtgtggttt cggctgtgaa gtcaggggca atatcgttaa
     4681 tgcgcaatgt catgcggcgt ctcctctgga gaattccatt tcgatgcagt ccaataaaaa
     4741 gcccacccgg tcagcgggtg ggttctgagg atcctgaaat ctcgatcgcc tctctcagaa
     4801 acacacccgc atgatgcagc cgcgacagca gccgcaggca cacatccggg tttgagaaga
     4861 agcgctcact cctcaacagc ctagggccag gcgatggtcg ttgtcaatcc agctgccagg
     4921 ctcgacaact atctcgtaag gctctctgcc agcgaagcca gctctctttt ggcgcggggc
     4981 ccgacccgaa ccgatgacaa cacggagcgg tgagactcac gtgatcgaga agcatctgca
     5041 ggccgattgc tcctggccac gcccacagcc cagcgcagcc gtttgtgcga gagcataaac
     5101 ttcacgatga agagactgcc accgtagacc gcgacaaaag tcagcgggag cagtacgaaa
     5161 atggcccgcg aactgttcac caaatcgact gatatgaagt ggggtagcgc tctaacaatc
     5221 acgaagcgaa aaaggacgag cgcaaccatg tgcgtcagat aaagtccgta agtgtgcgcc
     5281 cggacatcca cgaatcgcgg ccagacagct cgtttcagct tgacgatccc cagtacaacc
     5341 acgatggaag ccagttggtt ggtgatcctt agcgtgttca acgcatcttc ggagtgacga
     5401 gaaacgagca atcttgcctc tcccagcgcc agcacattcg tcaccagaat caacgcgatc
     5461 ataagagcca ttggaatgtg gctgagccac ttttcaatcg ctttaaagtg ccaggccccc
     5521 catgctccca gccaaagata gaaaacgaat ccaaacactg cacgggtgtg ttttaccggg
     5581 atccagccgc cgtagatgtt ggcggcataa aagaggctca gcaggaggaa ggccagaccc
     5641 atgcggtaat cccgaagtct gcggcgaaac accaatagaa cagccagcgc gattatcagg
     5701 ttgggaacaa accagtaggc ggtatccagc aggcctttcg aaaattcggc gtagacacgg
     5761 ctcacaagat cagcagagtg aaatgtcact cgcctgtgag cgaaatcggc ggtgagcgag
     5821 aggctgctgt aaaagccata ccagatcgtc catggcaggg ctacacttct cagccggcga
     5881 ctgaagtatt gcagagaact gtaatcgtca atgcgctcgc cgaagaggaa cccggcaacc
     5941 agaaaaaatc cgatggtccc gaacttcaga gcctgaagca accaggttgt ggaagcgccg
     6001 gagcaagtgt cgatggtgtg cgcggcgatg atcgctgcca tcgaaacaaa gcgcacgttg
     6061 ttggcgaaaa ggtgattggc gtcagtacct gacggtcgag tcgcctgtcc ggctgattgt
     6121 atgctggtag atttgtctgg gtcgacaggg caccgaacag cagcgacaat tgggcccata
     6181 gcaaaaaagc tccgatcaat atgatccgga gcttaatctg ctttgtggac acgcccaatc
     6241 agtaaagggg tcgcgctcat tccgccagta ctgaaaatgt gaggcacttc cgttgaaatt
     6301 cccaaaacag gaatgcaaat gcccagtctg cgcccaaacg ttcggacggg gaatgcgtgt
     6361 gattcagcga gcttaccgtg gattctgccc cagtacctgg taattgtggg caatctgcat
     6421 cgacatgacc cccacatagg caaactgggc aacgcgggcc agcgggcggt gattgtggcg
     6481 aaccaaatag cggtaggctt cgaaattcag ggcgaccgta ccggcttgaa atgccgcaaa
     6541 cgccggctta ttcttcacca ggccattggg cagcatcgcc tcgtggtagt actgcggttc
     6601 tgtaagcgcc ttctccgtgg tggtaaagtc cagcacccgc agggtcgcag ttgcccccag
     6661 aagactccag tccagtaaat tcaaccgggg cttcttttcc tgcacctgtg gggcctcgac
     6721 tggaaaaaca cccatcgcgg agccactgaa atcgtcgccg ggctgcgcgt cggacgcgac
     6781 gttgggcaac ggagcgttgg ccagcatggc ggtttgttgt gcgggacaac tggcgatgca
     6841 caggaacgag agaactggca gaaacagaac agcagagggg agtttcatgt tttcctcatc
     6901 ggcttgaacg gcctgtactg ttccggatgt gtcgaaaaga aggcaggaac aaatacaagt
     6961 cgtgcagtga gtttggctcg tggcatgcgg tttggggatt cgtggcgttg agcctttgtg
     7021 ccacccccgc aggtctcttc aagtgatgag tgagtgtggc acagaaatcg tatcgttgtg
     7081 cacttatctt gcgtcacttc aacaagttac gggagcgcta ccggttcata gctcttcagt
     7141 gaacggagcg tgtgactgtc acgctaaaaa tcagttacca gtgcggcaga aaaatgcaac
     7201 agcaatgatt accgatgcgc gacagcctat tcgggtttgg atgcccaaat gaaatgaaat
     7261 gagaaaaata gcggatggcg cgcaggtaac catggcgggg aacggtggtg ttttgggtgg
     7321 aattggaaag ttcctgaccg aacgcgcagc gaacaggtaa cggtaaccac tccgttgtta
     7381 atgcggaata ctccagcggc aagatgagtt caggatcagc gccgcacgct ctcgcgggtt
     7441 cagctctctg ccgttcgagc gaagattcca aactgcggat agcgtttctg gcgcacaatc
     7501 cagcgcgagc agcaagttca gctctgcgct cgaaccagtt tctacggagg agcgccaccc
     7561 tatgcagaaa gcagtgccgc tatcccagcc gcgatctgct gccgaactcg ctgcgatgcc
     7621 ggctgtagga gaagtaaatc cccaggccga tgaacagcca gataaagaaa gctgtccagg
     7681 tcaggacttc caaacccatc atcagcacga tgcagaagat gatggagagc accgggaaga
     7741 ccggccccag aggcgcccgg aaggcgcgcg gccgttccgg ttcgcgatag cgcaggatca
     7801 ggacggcgat tgaaaccagc acaaaagcga aaagagtgcc gatattggaa aggttcgaaa
     7861 ccgttccgat gtccagcagc ccggccggaa ttcccaccac gaatccagcc acccacgtcg
     7921 ccactgccgg cgtgcgaaat tgtggatgaa tgtggctgaa gatattcggc agcagccggt
     7981 cgcgcgacat ggcgaaccac acgcgcgcct ggccgagctg gaaaacgagg atcgaggaga
     8041 tcatgcccag catcgcgccg atgaggacga tcagttctac ccagcggagc gcatgtcctc
     8101 ctggcgcgac cgacagccgc ttcagtgcat tgacgaccgg ggccgcgtcg cccatgatcg
     8161 actgccaggg cacgatgcca ctgagcacga ttgcgacgcc gccatagagc aaagtgcagg
     8221 ccaccagcgt ggccagaatc ccgaatggca agtcgcgctg tggattgcgg cattcctcgg
     8281 ccgccgtgga aaccgaatcg aacccgatgt aggtgaaaaa gatgatcgac ccgcccgaca
     8341 acatgccggt ccatccgttc ggcatgaacg gatgccagta atgcggtttc acgaaacttg
     8401 cggcggcgaa gatgaatgcc aggatcgcga cgatctttac cagcacgaga atattgttcg
     8461 tgcgcgctga ctcgcggatg ccccgcacga ggaccacggt caggatcatc acgatgagga
     8521 atgccggaat attgaatccg aagtgccagc ctgggttata gagattgttg cccgcaaagt
     8581 cctgcagccc tgtcggcaga taggcaggcg agatccatcg cagtgccgga tggatgccga
     8641 accactcgac cagagcttcg atgtgtgccg cgaatcccac gctgacactc atatttgaga
     8701 atgcgtattc gagaatcagg tcccacccga tgatccacgc gatcagctcg cccatggttg
     8761 cgtaagtgta tgtgtacgca gagccggcga ttggaatcat tgacgccagc tccgcgtagc
     8821 agagtccggt aaagacgcac acaatcgcga ccagcaccag cgaaaccgcc aacgcgggcc
     8881 ccgcgcccgg gcggcccgcc atggccgtgt gggtaaggag gtagtggacc agaggggtgt
     8941 tgctgatttc cggtgagtcg aatttctgcc ctgccatcgc cgttccgatg acggtgaaga
     9001 tgcccgagcc gatcaccgcg ccgatgccga gtgcggtcag cgaaatcggt cccagggttt
     9061 tcttcagtgc atgttccgga cgttccgatt ccgtgatcag cttgtcgatc gatttgcgcg
     9121 cgaagatttg ccgcggttga cttccttcgg gccgggatgg aggcacgggc ggatcgggca
     9181 acgacagctc ctgcggggag gagtggggtg ttgtgtgagt gtaaaggttt cacggggcgg
     9241 gcggaagagg ttcgccagcc ccgtcggttt tcgctgcgga gaagggggcg ctctatcgct
     9301 tcgactgccc gcgcgccaac tttctcacct tctttttctg tgaacccctt ggagtcgtcc
     9361 gctttgcttt gggggcggcc ttcttacgcg ccgtgcgttt caccttcttg cgttctgttt
     9421 tatccgtaat cgatttcgta ttcgccatag gatccttttt cggagcaggt ttcggggcct
     9481 ttacagccgc tcgagctgtg cgaatttctc caccagcttt ttcactccga atcctgaaaa
     9541 ttgtaccgta atcttcgcat cgtctccgtc gccctcgcgc ctgaacacga tgccttcgcc
     9601 gtatttcggg tgccggaccc gctgcccgct gcggaaaccc ttcgcgccgg ttggttccgg
     9661 aacatccagc tttggccgcg agctccgcgc cggcacgctc cgtcccgcag gtccccgtcc
     9721 cgtgaagaac ttcgcaatgt tgtccaccga atcccgcggc cgctcattgc gcgcggcttg
     9781 tccagggcgc tccgtccgaa acgcggcgct ctgatcttca tcttcgtagc tgtagtgacg
     9841 cgagccgaat tcgtcatcac tgctgcggcc aaagcgcgat ggcgtctgcg agtactgcgc
     9901 cgcgtagccg ccccagcccg tcgatcgcgt tcgccccccg ctcaaatcct ccagcagatg
     9961 ctccggtatt tcctccagaa aacgcgacgc aatcgacgcc tccggcatat cgttcccata
    10021 ccgccgccga tatcgcgctc gcgtcaccac cagtgaatcc atcgcccggg tcagccccac
    10081 atagcacagc cggcgttcct cctccatctg cggcggatcg ttcagcgtcc gcgagtgcgg
    10141 gaacaaccct tcctccatcc cggccagcac cacgagcgga aactcgagtc ccttcgctgc
    10201 atgcagcgtc atcagggtca cgcgcgcctc cggatcgtac tggtctacat cgctcaccag
    10261 cgccgcgtga tccagaaact ccgcgagcgt ctcgccgcgc tcgtgcgcat cctgtgccgc
    10321 attggccagt tccttgaggt tttcaatccg cgaaaaggcc tccggcgtgc cttcctcctc
    10381 cagcgatttg atataccccg tccgatcgat gacgaacttg atcagctccg gcagcgtcgc
    10441 ggccccaccc ggcttcctga atcctggaac agcttcgtct tccgatccct gagctctgag
    10501 ctctgagttc tgtgcctggg catgctctac cggcgcctct tctgcgaaca ccgagaactt
    10561 cgccggctca aaatcttctg cgctctctga tatgtaatca tcggccgcaa ggccagggtc
    10621 ggcgtcgtgg gtcaaggcca gcatcccctg cgcaccgaag tcgaagctcg tgtcgtcgac
    10681 cgcagcgaac tcggtagtcg cctgcggagc cacatccgcg gtcagcttct ccacaaatcc
    10741 ggctcccacc atcgcccgcg catcggtgat gatccgccgg aagccatcca gagcctgcag
    10801 cgatcgctgc ggcagcagct gcttctcgag cgcgcgatcg atccccgtcc agatgctcga
    10861 tcctgtttcc agggcgatcc gctccagagt ctccatcgtc gtctttccga ttccgcgcgc
    10921 gggcgtgttc accacccgct gcagcgcgat cgagtcgtgc ggattcgcca ccagcttcag
    10981 atagctcagc agatccttaa tctccgcgcg ctcgtagaat gaaaacccgc ccaccatcgt
    11041 gtaggggatg ttgtaccgtc gcatcgcctc ttcaatcagc cgcgactgtg aattggtccg
    11101 gtacagcacc gccgctttgc cgccatctcc gccctcttcg cccgccttgc gcaaatactt
    11161 ctgaatcgaa tccgcaacga aaagcgcctc gttctcaccg tccggagcct cgtagtatcc
    11221 gatcaatgca ccgccctggc gggaggtcca cagccgcttg cctttgcgct gcgcattcct
    11281 cgccaccacg gccgatgctg cttccagaat gatctgggtc gaccggtaat tctgctccag
    11341 ccgcaccgtc ttcgcgcccg gaaaatcctt ctcgaactca aggatgttgc gaatgtccgc
    11401 gccgcgccac gagtaaatgc tctgatcctc gtcgcccacc acgcagacgt tcttctcctc
    11461 gcccgcaagc agcttcatca gttcgtattg cggccggttg gtgtcctgat actcgtcgat
    11521 c
//
LOCUS       DASZYN010000621         4467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_482108, whole genome shotgun sequence.
ACCESSION   DASZYN010000621 DASZYN010000000
VERSION     DASZYN010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482108"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VHE33_15930"
     CDS             complement(<1..434)
                     /locus_tag="VHE33_15930"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HVT98994.1"
                     /translation="MRKTLLLPSILIALLGMSLGCSRQAAPPSDQQLTGSVQAKLQSE
                     SALTGQNIQVAVNHGVVTLSGTAADPASRALAGNDAGAVPGVTTVVNNLTVQPQIASA
                     TTPETQPQLRPDSAPPAPRKEPRHVTPPRHESPAPPPPPPAET"
     gene            742..1134
                     /locus_tag="VHE33_15935"
     CDS             742..1134
                     /locus_tag="VHE33_15935"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HVT98995.1"
                     /translation="MADEKQSSGFSWFLAGLGLGALIGVLYAPKAGRETREELMSSAR
                     EGTEYLKQKSRDAVDQVNDLVDRSKTQVNDYVGRSKEYVERGRAQWDEFVNQGRQFVN
                     DQTGRVSAAVDAGRQAYQTNAGQNPEGM"
     gene            1234..1767
                     /locus_tag="VHE33_15940"
     CDS             1234..1767
                     /locus_tag="VHE33_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98996.1"
                     /translation="MSHNIPEWVYITFTAVTAVGVLMQALVLLGMLLALKGALKRVNE
                     VTAKAEDIAVPAFESTRDLLKDISPKLKVAAQNLMEVSQTAKEVSNTVRAESTQMAVA
                     LNDVLARASVQVDRVDEMVTGTLSSVAHATSTLQRAVSGPARQVGALLSGLRAGFDVL
                     RKKENGVHAAADGDHFV"
     gene            1967..>4467
                     /gene="treY"
                     /locus_tag="VHE33_15945"
     CDS             1967..>4467
                     /gene="treY"
                     /locus_tag="VHE33_15945"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HVT98997.1"
                     /translation="MKDCIEQTPRMEARAPLSTYRLQLHAGFTFADAEAVLPYLHALG
                     IGDCYASPIFEARPGSMHGYDVTRHDRLNPELGGEEGFARFSARLRALGMGLLLDIVP
                     NHMGVGNDSQWWQDVLENGRASQYASYFDIDWNPLNPDMKDKLLLPILGSQYGEELEA
                     KRIQVVLVDGKLMVRYFDHLVPIAPRTIQAIFPTTELDELGVPQSFREILRETAHIPP
                     HDTTDTTLVAQRRQQLAELKPRLREALLAPEMQPILGKALERINGIAGDPRSFDRLHD
                     LLEQQPYRLAYWRVSSEEINYRRFFDINDLVGLRMENGDVFAETHCLIRRLLARGDVT
                     GLRIDHADGMFNPRQYLIRLQLLYVAAHCCGETPQGPTGENGIEDEVRDAMRGCDWST
                     TQGPLYVVVEKILEPRESLPREWPVRGTSGYDFVYLGNQIFIYRENEGRFTDFYQRVT
                     GETKDPETIIYESKLNVMRNALSSETYVLTNLLSRLASANRQVRDFTDDLLETAIRET
                     IACFPVYRTYIDDRGQYTERDRAFTRLAVARAKRRNRETDESVFDFLERTLLLHGQMA
                     DEIDPAQLYFALKFQQLTGPVMAKGVEDTAFYVYNRFLASNDVGSSMEAFGIGLEDFH
                     DASRARLQVAPDAMLATSTHDTKRSEDVRNRLDVLSEMTYVWPSYVRRWMRLNARHKR
                     TLSDGRVAPSANEEYFIYQTLAGAWPWQMRTRENREEFLERMQQYMTKALSEAKVNLS
                     WTNPNPAYVDAVHHFLRAILMRDARGREPRFVETLGEILPALQLFGAVNSLAQVVLKV
                     ASPGVPDFYQGTEMWDLSLVDPDNRRPVDYEVRRQA"
BASE COUNT          882 a         1321 c         1447 g          817 t
ORIGIN      
        1 gtctcggccg gtggcggcgg tggaggtgcc ggagattcgt gccgcggagg cgtcacgtgg
       61 cgaggttctt tccggggagc cggaggagcg gaatcgggcc gcaactgcgg ctgcgtctcg
      121 ggcgttgtcg cggaagcgat ctgcggctgg acagtcagat tgttgacgac cgttgtcact
      181 ccgggcacag cgccggcgtc gtttcctgca agagcccgcg aagcgggatc ggcagccgtg
      241 ccgctgagcg tgaccacacc gtggttcacg gcgacctgaa tgttctgccc cgtgagcgca
      301 ctttcacttt gcagttttgc ctgaaccgag cccgtgagct gctgatcgga gggcggagcc
      361 gcctggcggc tgcacccgag actcattccc aacagggcaa taaggatgga aggaagaagg
      421 agggtctttc tcatgctgat tcctttcgcg gacatgcgcc gcacaattcg cgattgagct
      481 catgaacgcg cgcgggaagc ggtatataac cggtgtatat tagacgcgcc tggaactccg
      541 gcagcgtcag aaaacgcgac cgcatcacac tgagtgtagc tgcaggaaca agaggcgcgg
      601 agagggtcgt cgcaggtttc cggtgagcaa aagagcgacg gaggagctgc gtggagtgtg
      661 cggggctcag tttgcagcgc ctgcacgaag ccagtaccat tgcatttggc gaatcggttt
      721 cattcttaag gagataaagt catggcagat gaaaaacagt ccagcggctt cagctggttc
      781 ctggcaggat tgggcctcgg tgccctgatt ggcgtgctct acgcgcccaa ggcgggcagg
      841 gaaacgcgcg aagaattgat gagcagcgcg cgtgaaggaa ccgaatacct gaagcagaag
      901 agccgcgatg ccgtcgatca ggtgaacgat ctggtggatc gcagcaaaac acaggtgaat
      961 gactatgtcg gccgcagcaa ggaatatgtg gaacgcggac gcgctcagtg ggacgaattc
     1021 gtgaatcagg gacggcagtt cgtgaatgac cagaccggtc gcgtctccgc cgcagtggac
     1081 gccggacggc aggcctacca gaccaacgcc ggccagaatc cggaaggtat gtaagtctga
     1141 gtcgcgtggc tggcgctggg cacagcgccg gtcagagcac tgtgtggtgc gctgacagcc
     1201 cgtcgcggcg gcggattcag cagggagttg agcatgtctc acaatattcc ggagtgggtt
     1261 tacatcacct tcaccgcagt gacggctgtg ggcgtgctga tgcaggcgct ggtgttgctg
     1321 ggcatgctgc tcgccctcaa gggtgccctg aagcgagtga atgaagtgac cgcgaaggct
     1381 gaagacatcg cggtgccggc gtttgaatcc acgcgcgatt tgctgaagga tatttcgccc
     1441 aagctgaaag tggcggcgca aaatctgatg gaagtgagcc agacggcgaa agaagtgagt
     1501 aacacggtgc gcgccgaatc cacgcagatg gcggtagcgc tgaacgatgt gctcgcgcga
     1561 gcctctgtcc aggttgatcg cgtggacgag atggtgaccg gcaccctgag cagcgttgcg
     1621 catgccacgt caacattgca gcgagcggtg tccggcccgg cgcgccaggt aggagccctc
     1681 ctgagcgggc tccgggcggg gttcgacgtt ttgagaaaga aagagaatgg ggttcacgcc
     1741 gcggctgacg gcgaccactt cgtttagaag caccgcattc tgatccgttc aaaggttcac
     1801 actgcctggc attcgtgaag cgatgtgtcc ccgtcggggt gcggggattc gcgttcggcg
     1861 gagggcagca cgttgacctc gagtaacacc ccgcccgggc tgctcccggc cctctccctg
     1921 catccaaccc agcaccagga attcaaccca ggatccctct cgaaaaatga aggactgcat
     1981 tgagcagacg cctcgaatgg aggcgcgcgc gccgctgtca acctaccggc tccagctcca
     2041 cgcgggcttt accttcgcgg atgccgaggc ggtgctgcct tatcttcacg ctttgggcat
     2101 tggggattgc tacgcctcgc cgatcttcga ggcccgcccc ggcagcatgc acggctacga
     2161 cgtcacccgc cacgatcgtc tgaatcccga gctgggcggt gaagagggat ttgcgcgttt
     2221 ctccgcacgc ctccgcgccc tcgggatggg cctcctgctc gacatcgtgc ccaaccacat
     2281 gggcgtcggc aacgattcgc agtggtggca ggatgtgctg gaaaacgggc gggcctcgca
     2341 gtacgccagc tatttcgaca tcgactggaa tccgctcaac ccggacatga aggacaagct
     2401 cctgttgccg atcctgggct cgcagtatgg cgaggaactg gaggcgaagc ggattcaggt
     2461 ggtgctggtg gacgggaagc tgatggtgcg gtacttcgac catctggttc ccattgctcc
     2521 gcgcacgatt caggccattt tccccacgac agagctggat gagctcggcg tgccgcagtc
     2581 ctttcgagag atcctgcgcg agaccgccca tattcctccc cacgacacca ccgacacaac
     2641 cctggtggcg caacggcgcc agcagctggc agaactgaag ccccggttgc gagaagcgct
     2701 gctcgccccg gagatgcagc cgatcctcgg caaagcgctg gagcggatca acggaatcgc
     2761 cggcgatccg cggagtttcg accgcttgca cgacctgctg gagcagcagc cctaccggct
     2821 ggcctactgg cgcgtctcga gcgaggagat caattaccgc cgcttcttcg acatcaacga
     2881 cctcgtgggt ctgcgcatgg aaaacgggga cgtctttgcc gaaacgcact gcctcattcg
     2941 ccgtctgctg gcgcgcggcg atgtgaccgg gctgcgtatc gatcatgccg acggaatgtt
     3001 caatccccgg caatatttga tacggctgca gttgctttat gtagcggcgc actgttgcgg
     3061 cgaaaccccg cagggaccga ccggcgagaa cggcatcgag gacgaagtgc gcgatgcgat
     3121 gcggggctgc gactggtcca caacccaggg ccccctgtat gtggtggtgg aaaagattct
     3181 cgagccgcgc gaatccttgc cccgcgaatg gccggtgcgg ggaacctcgg ggtacgactt
     3241 cgtttacctc gggaatcaga tcttcatcta ccgcgaaaac gaggggcgct tcactgactt
     3301 ttatcagcgg gtcaccggag aaacaaagga cccggagacg atcatctacg agtcgaagct
     3361 gaacgtgatg cgcaacgctc tgtccagcga gacgtatgtg ctcaccaatc tgctgagccg
     3421 gctggcgtcg gcgaatcgcc aggtgcgcga ctttaccgac gatctgctgg agactgcgat
     3481 ccgggaaacg atcgcctgtt ttccggtgta tcggacctac atcgacgatc gcggccaata
     3541 caccgagcgg gatcgcgcgt ttacacgcct ggccgtggcg cgggcgaagc ggcgcaatcg
     3601 tgaaaccgac gaatccgtgt tcgatttcct cgagaggacc ctgctgctcc acggacagat
     3661 ggccgacgag attgaccctg cccagcttta tttcgcgctg aaattccagc agctcaccgg
     3721 gcccgtgatg gcgaagggcg tcgaggacac cgcattctac gtctacaacc gcttccttgc
     3781 ctccaacgac gtcggcagtt cgatggaagc gtttgggatc ggtctggagg acttccacga
     3841 cgccagtcgc gcgcgccttc aggtggcgcc ggacgccatg ctggccacct ccacgcacga
     3901 tacgaaacgc agtgaggacg tgcgcaaccg tctcgatgtg ctttcggaga tgacctacgt
     3961 ctggccgtcc tatgtccggc gctggatgcg cctcaacgcc cggcataagc ggaccctgag
     4021 cgacggacgc gtggccccca gcgcgaacga ggagtacttc atctaccaga ccctcgccgg
     4081 agcgtggccc tggcaaatgc gcacccgcga aaaccgcgag gagtttctcg agcgcatgca
     4141 gcagtacatg accaaagctc tcagcgaggc caaggtcaat ttgagctgga cgaatccgaa
     4201 cccggcgtac gtagacgcgg tccatcattt cctgcgcgcg attctgatgc gcgatgcgcg
     4261 cggccgcgag ccccgttttg tcgagaccct gggagagatt ctccccgctc tgcagctgtt
     4321 cggagccgtg aactcgctcg cccaggtcgt gctgaaagtg gcttcgcccg gagtgcccga
     4381 cttttatcag ggcacagaga tgtgggacct gagcctggtc gatccggaca accgccgccc
     4441 tgtcgactat gaggttcgcc gccaggc
//
LOCUS       DASZYN010000622         5606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1188442, whole genome shotgun sequence.
ACCESSION   DASZYN010000622 DASZYN010000000
VERSION     DASZYN010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188442"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="VHE33_15950"
     CDS             complement(<1..1131)
                     /locus_tag="VHE33_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98998.1"
                     /translation="MVLALLSMGALALHANDDAAGRAGYYREPALHGDTLIFASEGDL
                     WTVNLHGGAAHRLTSAPGQERKAKISPDGKTVAFYGDYEGPGEVYTMPVEGGVPERQT
                     WESGAEPAGWNPDGRLLIETRRYATLPDPSLVLIASHGERERVPLATGSEACYSTDGK
                     TLFFTRIDKQWSNTKRYKGGFIQNIWRYDGQGEASLLTGGYAGTSHAPMFWNGRVYFL
                     SDRDGVMNVWSMDGDGRGLKQESHQKFFDVESASVWDGKAVYASGGDLWLLDLKTGQE
                     AVIPITLVSDFDQMRQHWVKKPLSFLTDTHLSPDGSRVVFTARGEIYTLPARDGRIIR
                     VAGSDSGVRYRAARFMPDGKSIVALSTKTGETEFWKFPANGEG"
     gene            1315..2646
                     /locus_tag="VHE33_15955"
     CDS             1315..2646
                     /locus_tag="VHE33_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT98999.1"
                     /translation="MAFDPAQLQIPALYPAPSALYPVFVIPSLRSAFNAHFRPEAFRN
                     LLHSLDAIARTHIAFPVAETPCFFPQSLLDEMARIGAELTHQLVSDTAYLTRSLAAIP
                     ERWRCADQNARPHFLTADFGLVRNPDGSIAPRLVEIQAFPSVFGFQFAASEAYRTAFG
                     LDSSLEYLLGGLDESSYWKLLSRTIVAGHDPETVVLIDVDPANQKTLPDFHITADRLG
                     IRIVDIATLEPIDGTSKSPGGRRRLAYRNGARLVPIRRIYNRAIVDELIRKQIPLQFD
                     YRESFDVEWAGHPNWYFHISKFSIPFLDHPAVPPAFFLDQLYRDSGLRDRFEANRNGW
                     ILKPLFSFAGQGIRFAPTDADLAAIPSRDRANYLLQERVTFVPVIDTPLGPTQAEIRI
                     LYVWPDRAALTPLTSLVRLGRGPMMGVDHNQGQAWVGASAAFFPTQTSTQR"
     gene            2829..3164
                     /locus_tag="VHE33_15960"
     CDS             2829..3164
                     /locus_tag="VHE33_15960"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HVT99000.1"
                     /translation="MESALCSSHGTSPWHHYRGAARYPVRLRVTVLAEGHDTEAFTED
                     LSASGCLLRLREPLRERQQIDFLVEIPAGTLDFSVTAAVHCSGSIVRSYWNNGQPFAA
                     VVIDEYRFQ"
     gene            3179..3928
                     /locus_tag="VHE33_15965"
     CDS             3179..3928
                     /locus_tag="VHE33_15965"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT99001.1"
                     /translation="MTQTNGNGNGVPPQAAQPSPEVRVILADSQAIYRVGMRKVFGPD
                     SGVVVAAEADNLQGLQDTVQRVPADVLLLEGAMITGTANAVPAIVRLAPDLKIIVQSA
                     NTDESHTVDLYRRGVRGIIPRSISPDLLVKCVRKIASGETWIDNQSVTWVIEAYRSQA
                     GNMNNPRNQPRLNQKEMLIIAGITQGKRNKEIAYQLGTTEQVVKNYLRKIYDKLGVSD
                     RLELALYCLHHQLHKTAAEADSRPLEVSTQP"
     gene            complement(3985..4299)
                     /locus_tag="VHE33_15970"
     CDS             complement(3985..4299)
                     /locus_tag="VHE33_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99002.1"
                     /translation="MTRSGLYRYPFDKDGAPVLTFGEAIAAARNLEAQLNILLKRWTW
                     PDVTLTIFERRPGYVRYEVRRQGEYVGIATVMPFVTEAERIGPEGVSEGKRQKASGTD
                     GD"
     gene            complement(4457..4642)
                     /locus_tag="VHE33_15975"
     CDS             complement(4457..4642)
                     /locus_tag="VHE33_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99003.1"
                     /translation="MKIFEIVFGCRHTELSRVFTIGGQSYCVCWTCGSRFSYSLASMR
                     MGRRFADVHEALPARSA"
     gene            complement(4680..4862)
                     /locus_tag="VHE33_15980"
     CDS             complement(4680..4862)
                     /locus_tag="VHE33_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99004.1"
                     /translation="MKQDRKRVLPMWRDAEGPAAQTAESAGAWTLVDYLFPAERGPVW
                     CGSARAESEIVLAKTA"
     gene            complement(5010..>5606)
                     /locus_tag="VHE33_15985"
     CDS             complement(5010..>5606)
                     /locus_tag="VHE33_15985"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HVT99005.1"
                     /translation="SPGPDYPQLPNAPVEMREIEKHFRPQDATVFARGRANAAAYLKS
                     DPRRFAYIDFVAHGVASRTDPLDSAIILSRTPGSEDSFKLYAREIMQHPIGARLVTIA
                     ACYGGGTRSYAGEGLVGLSWAFLRAGAHNVIGALWEVSDASAPRLMDVLYQGLQDGLP
                     PSAALRQAKLQLLHGTGAERGEFRRPFYWGSFQLYSVQ"
BASE COUNT         1042 a         1977 c         1425 g         1162 t
ORIGIN      
        1 gccctcgccg ttcgccggaa acttccagaa ctccgtctcg cctgtcttcg tcgacaaagc
       61 gacgatgctc ttgccatccg gcataaagcg cgccgcgcga taccgcactc ccgaatcgct
      121 gcctgctacg cgaatgatcc gtccatccct ggccggcagg gtgtagattt ccccgcgtgc
      181 tgtgaagacc acccggcttc catccggcga caggtgcgta tctgtcagga atgagagcgg
      241 ctttttcacc cagtgctgac gcatctggtc gaaatccgag accagcgtga tgggaataac
      301 ggcctcctgc ccggtcttca ggtccagcaa ccacaggtct ccaccgcttg catagaccgc
      361 cttgccgtcc cagaccgatg cactctccac gtcgaagaat ttctgatggc tctcctgttt
      421 gagcccgcgt ccgtccccat ccatcgacca gacattcatc acgccatcgc gatctgaaag
      481 aaagtaaacg cgtccgttcc agaacatcgg cgcatgcgat gtccccgcat agccgcccgt
      541 cagcagcgac gcctcaccct gcccgtcata ccgccagatg ttctggatga atccgccttt
      601 gtagcgcttc gtattgctcc actgcttgtc gatccgtgtg aagaacagcg tcttcccgtc
      661 cgtcgaatag catgcctcag aaccagtcgc cagcgggacc cgttcgcgct cgccatggct
      721 cgcgatcagc accaggctcg ggtcgggcaa agtcgcgtac cggcgcgtct caatcagcag
      781 ccggccgtct gggttccacc ccgcgggctc ggcgccgctt tcccaggtct gccgctccgg
      841 aactccgccc tcgaccggca tcgtgtatac ctcaccgggc ccctcgtagt cgccatagaa
      901 cgcgaccgtc tttccatccg gcgaaatctt cgctttgcgc tcctggcccg gcgcagaggt
      961 cagccggtgg gctgcgcctc cgtgcaggtt caccgtccac aaatcgcctt cagaagcaaa
     1021 gatcagggtg tcgccatgga gcgccggttc gcgataatag cccgctcgtc cggccgcatc
     1081 gtcgttcgcg tgaagagcca gagcacccat cgaaagcaaa gcaagaacca acgcacttcg
     1141 gcggattccg tgcacagaga acatacggac ctgatacctt tcgctttcat gagccggcaa
     1201 ctccgcctca cgaatggaag aaattaagac aggggactga tgacgttaca ctctatcact
     1261 ctgcgtcacg ggcctatcca cctccgtgat gaagccgccc tggaatgacg cggcatggct
     1321 tttgatccag cacaattaca aatcccagcc ctgtaccctg caccttccgc cctgtaccct
     1381 gttttcgtga ttccgtccct tcgcagcgcc ttcaacgcgc acttccgccc cgaagctttc
     1441 cgcaacctgc tgcactcgct cgacgctatc gcgcgcaccc atatcgcgtt ccccgttgcc
     1501 gagacgccct gtttcttccc ccagtcgctc ctcgatgaaa tggctcgtat cggcgccgaa
     1561 ctcactcacc agcttgtcag cgatacggct tatctaaccc gctctctcgc ggccatcccc
     1621 gaacggtggc gctgtgccga ccagaatgcg cgcccgcact ttctcaccgc cgacttcggc
     1681 ctggtccgca atcctgacgg ctcgatcgct ccacggcttg tcgaaatcca ggccttccct
     1741 tcggtgttcg ggtttcagtt cgccgccagc gaagcgtatc gcacggcttt cgggctcgat
     1801 tcctctctcg aatacctcct cggcggcctc gacgaatcgt cctactggaa gctcctcagc
     1861 cgcaccatcg tcgccggcca cgatccggaa accgtcgttc tgatcgatgt cgatcccgca
     1921 aaccagaaaa ctctgcccga cttccacatc accgccgatc gtctcggcat tcgcatcgtg
     1981 gacatcgcca ctctcgagcc catcgacgga acctcgaagt caccaggcgg gcgtcgccgt
     2041 ctcgcgtatc gcaatggtgc ccgcctcgtt ccaattcgac gcatctacaa ccgcgccatc
     2101 gtcgacgaac tcatccgcaa acaaatcccg ctccagttcg actaccggga atctttcgac
     2161 gtcgagtggg ccggccaccc caactggtac ttccatatca gcaagttctc catcccgttt
     2221 ctcgaccatc cagcggtacc gccggccttc tttctcgatc agctctatcg tgattccggc
     2281 cttcgcgacc gcttcgaggc caaccggaat ggctggatcc tcaaacccct tttctctttt
     2341 gcaggccagg gtattcgctt cgcccccacc gacgccgacc tcgccgccat tccctccagg
     2401 gaccgcgcga actacctact ccaggagcga gtcaccttcg tacccgtcat cgatacccct
     2461 ttggggccaa cccaggccga aattcggatc ctttatgtct ggcctgatcg ggcagccctc
     2521 acccctctaa cctccctggt gcgccttggc cgaggtccaa tgatgggtgt cgaccataac
     2581 cagggtcagg cgtgggtagg tgcctcagcg gctttctttc cgacccaaac gtctacccaa
     2641 aggtaatttg tgcctcgaaa tccaccagca acccaccttt cccacagatg cccgcgcccc
     2701 tgccccattt tggtcattgc cacaccccat ttggtcgttt acaaatggga acgaagatgt
     2761 aaactttggt aattggatag cgccattgtt tgcgggtact gtagggatta acattcgcag
     2821 gcctttctat ggaatctgcc ctctgttcat cccacggcac cagcccctgg caccattacc
     2881 gcggcgcagc ccgctacccg gttcgacttc gggtaaccgt gctcgccgaa ggccatgata
     2941 cggaagcgtt caccgaggat ctctccgcca gcggttgtct cttacgctta agagagccac
     3001 ttcgggaacg gcagcaaatt gatttccttg tggagattcc ggcgggcacg ctggatttct
     3061 ctgtgactgc ggcggtgcac tgctctggaa gtattgtccg ttcttattgg aacaatggtc
     3121 agcccttcgc cgctgttgta atcgacgagt acaggttcca gtagtcggac aaacatttat
     3181 gacgcaaacc aatggcaacg gcaacggtgt acccccccag gcggctcaac cctcccctga
     3241 ggttcgggtg attctcgctg actcccaggc aatctatcgg gtcggcatgc gcaaagtctt
     3301 cggacccgat tcaggggttg tagtcgctgc cgaagctgat aacctccagg gcctccagga
     3361 caccgtccag cgtgtccctg ctgacgttct gctcctcgaa ggggccatga tcaccggcac
     3421 tgctaacgcg gttcctgcga tcgtacgcct cgctcccgat ctcaagatca tcgttcagtc
     3481 cgccaatacg gacgagtcgc ataccgtcga tctttaccgg cgcggcgtcc gcggcatcat
     3541 tcctcggtct atctccccgg atctgcttgt caagtgcgtg cgcaagatcg catccggcga
     3601 gacctggatc gacaatcagt ccgtcacctg ggtcatcgaa gcctaccgtt cccaggctgg
     3661 caacatgaac aacccgcgca atcagcctcg cctcaaccag aaagagatgc tgatcatcgc
     3721 cggcattacc cagggcaagc gcaataagga aatcgcttac cagctcggta ccaccgagca
     3781 ggtcgtcaag aattatctgc gcaagatcta cgacaagctg ggtgtttccg atcgactcga
     3841 gctcgcgctc tactgcctgc accatcagct gcacaaaacc gcagccgagg cagactcgcg
     3901 ccccctcgaa gtgtctaccc aaccctgatt ttttgggaga atttaagact ccggtacagg
     3961 gcatgacctt cggtctgccc tgtttcagtc tccgtcggtt ccgctcgcct tctgccgttt
     4021 cccttccgaa accccctccg gcccgatcct ctccgcttcc gtcacaaagg gcatcaccgt
     4081 cgcgatcccg acgtattccc cctgccgccg cacttcgtag cgaacataac ccggccgccg
     4141 ctcgaaaatg gtcagtgtca cgtccggcca cgtccagcgc ttcagcagaa tgttcagctg
     4201 cgcttccaga ttgcgtgccg cggcgatcgc ctcgccgaac gtcagcaccg gagcgccgtc
     4261 cttatcgaat gggtaacgat agagccctga gcgtgtcatt ctttgtatct ctatctttcg
     4321 cgctcgggtc catccccgtc acttccccgt tagccatctg tcgcccacat cttggtaacg
     4381 tttcaccggc gaccgcagga accacttccg tagtacttgc gggcttgcgc ccggcgtctc
     4441 gctatttacg caggcttcac gcagaacgcg ccggcaaagc ctcatgtacg tcggcgaacc
     4501 ttcttcccat gcgcatgctc gcgagcgagt agctgaacct cgacccgcac gtccagcaca
     4561 cgcaatagct ctgtccgccg atggtgaaga cccggctcag ctccgtatgc cggcatccga
     4621 agacaatttc gaagattttc atcgttccct ctcctgtcgt attcctcggc cgatcgcctt
     4681 tacgctgtct tcgccagaac gatctcagac tcggcgcgcg cggatccgca ccacaccggc
     4741 cctcgctcgg ccggaaacaa ataatccacc agcgtccatg ccccggcgct ctccgccgtc
     4801 tgcgctgcag ggccttctgc gtcccgccac atcggcagca ccctcttgcg gtcttgtttc
     4861 acgctgctcc tccgtgccgc acctggcgga catccgctac cggagtcgcg ctctcaggtc
     4921 acactttgga ttagtgacgg gaaaacggct tgcgtatcag ccaggccgat caggcttccg
     4981 ggtgccgagc gcgaaacccc gagcgccgct cactgcacgc tgtacagctg gaacgatccc
     5041 cagtagaacg gcctgcgaaa ttcgccgcgt tccgcaccgg tcccatgcag cagctgcaac
     5101 ttcgcctggc gcagcgccgc gctcggcggc aatccatcct gcaggccctg gtagagcacg
     5161 tccatcagcc tgggcgccga ggcgtcgctc acctcccaga gagcgccgat cacgttgtgc
     5221 gcgcccgcgc gcaggaacgc ccacgacaac ccgaccagcc cttcgccggc gtacgatcgt
     5281 gtcccaccgc catagcaagc tgcaatcgtg accagccgcg caccgatggg atgctgcatg
     5341 atctcgcgtg cgtacagctt gaaggagtcc tccgaccccg gtgtccgcga caggatgatc
     5401 gccgaatcca gggggtcggt ccggctcgcc acgccatgcg cgacaaagtc gatgtaggca
     5461 aaacgccgcg ggtcgctctt cagatacgcg gctgcatttg cccgtccgcg tgcaaacacg
     5521 gtcgcgtcct gcggccggaa gtgcttctcg atctcccgca tctccacggg cgcgttgggc
     5581 agctgcggat aatccggacc cggcga
//
LOCUS       DASZYN010000623         3338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_673254, whole genome shotgun sequence.
ACCESSION   DASZYN010000623 DASZYN010000000
VERSION     DASZYN010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673254"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="VHE33_15990"
     CDS             complement(<1..1431)
                     /locus_tag="VHE33_15990"
                     /inference="COORDINATES: protein motif:HMM:NF005546.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HVT99006.1"
                     /translation="MKTAVIIGAGPAGLTAALEFLRRSGVRPIVLEATQRIGGISCTI
                     CHNGNRIDIGGHRFFSKADRVMDWWTDLLPIASTGNDSESHTLRYQNRERTVSAGMDA
                     ATDPDLVMLVRPRKSRIYFLRSFFDYPLALNAQTLRQLGLGRTFRIGIGYLRARLFPR
                     RNEKTLEDFLINRFGTELYLTFFKSYTEKVWGVPCNQISAEWGAQRIKGLSLRTAVAH
                     FLKKAFSPKTFGDLSQKSTETSLIEKFLYPKYGPGQLWEYVASLVTKKGGEVHLGWRA
                     TKLHIEGDRVVSVAARDPWGAMHTIPADYVFSTMPVRELIHSLDVDVPADVKAIADGL
                     IYRDFITVGLLVDRLTVTEPDGGPLKDTWIYIQEPDVLVGRLQIFNNWSPYMVADPDK
                     VWIGLEYFCYETDQLWNMPDDEIARFAIAEVEKIGILRASQVRDSVVVRVPKTYPAYF
                     GTYDRFDEIVRYTDRFENLFMVGRNGM"
     gene            complement(1611..1901)
                     /locus_tag="VHE33_15995"
     CDS             complement(1611..1901)
                     /locus_tag="VHE33_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99007.1"
                     /translation="MARGVSFTGEFLTRYLSAQKRWQEAREWRAKETEAGRPSAWQDF
                     CRAHGLCPACAASGISLNANGVGFKTLGWNGNLPLYEECEACGGAGKIVNPS"
     gene            complement(1932..>3338)
                     /locus_tag="VHE33_16000"
     CDS             complement(1932..>3338)
                     /locus_tag="VHE33_16000"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99008.1"
                     /translation="TALFICIFILVPFTGLYTFLGGLWGVLVTDLFQFVLKMAMIIVL
                     AWIAVVRLGGMSALRVRLNIVDAATRAAGHPTSSVLSFLPDFHSGLTTSNLWTLPLLT
                     FLMYIGVQWWAAWYPGAEPGGGGYIAQRMFSAKNEKHSLGATLWFNIAHYALRPWPWI
                     VTGLVALAMYSTHGGLHPSAAYSANPQQGYVMVLRDFLPPALRGLMIAAFLAAYMSTI
                     GTQLNWGTSYLVSDLYRRFMVRDANERHYVFVSKIITVILVLVSGYVASRLTSIGAGW
                     ELVLNLGAGTGAVYILRWFWWRINAWSEISAMIVAAVVALSLSRVHFSGNDAVIFAKS
                     TLITAGVTTVAWIAATFLTRAEPDATLLAFYRRVHPMVHGWKHIASQAPELPPVRDFR
                     ANAFDTILGCILVYGCLFGIGKLIFGAWLAAFVLLAAAAIAGWLIWWDLSRRGWETLS
                     GASPSSVAENTLSPTVKS"
BASE COUNT          731 a          848 c         1157 g          602 t
ORIGIN      
        1 catgccattg cggccgacca tgaatagatt ctcgaatcga tccgtatagc ggacgatctc
       61 gtcgaagcga tcgtaggtac caaagtaggc gggatacgtt ttcggaacgc ggacgacgac
      121 cgagtcgcgg acctggctgg ctcgaaggat gccgatcttc tcgacttcag cgatggcgaa
      181 gcgagcgatc tcgtcatcgg gcatattcca tagctggtcg gtctcgtagc agaagtattc
      241 gagtccaatc cagactttgt caggatcggc gaccatgtac gggctccagt tgttgaaaat
      301 ctggagccgg ccgacgagga cgtcgggctc ctggatgtag atccaggtgt ctttgagcgg
      361 gccaccatcg ggttcggtaa cggtaaggcg gtcgacaaga agaccgacgg tgatgaagtc
      421 gcgatagatg agaccgtcgg cgatggcctt gacgtccgca ggaacatcga catcgaggct
      481 gtggatgagt tcacggaccg gcatcgtcga aaaaacgtag tcggcaggaa tggtatgcat
      541 ggctccccag ggatcgcgcg cggcaacgga gacgacgcgg tcgccttcga tgtgcagttt
      601 tgtggcgcgc caacccaggt gcacttcgcc gccttttttt gtcaccaggc tcgctacata
      661 ttcccagagt tggcccgggc cgtatttcgg atagaggaac ttttcgatca gcgaggtctc
      721 cgtggacttt tgggaaaggt ctccgaacgt tttcgggctg aaggcttttt tgaggaaatg
      781 ggccacagct gtgcgcaggg agaggccctt gatgcgctgg gctccccact cggcgctgat
      841 ctgattgcac ggcacgcccc agactttttc ggtgtaggac ttgaagaagg tcagatagag
      901 ttcggtgccg aagcggttga tgaggaagtc ttcgagagtt ttctcgttgc ggcgcgggaa
      961 cagtctggcc cgcagataac cgatgccaat cctgaaggtg cgccccaggc cgagctggcg
     1021 aagggtctga gcattgagcg caagggggta gtcaaagaag ctgcgcagga aatagatgcg
     1081 gctcttgcgc ggccgcacca gcatgaccag atccggatcg gtagcggcat ccattccggc
     1141 tgacacggtg cgctcgcgat tctgatagcg gagggtgtgg gattcggaat cgttgccggt
     1201 ggaagcgata ggcagcagat ccgtccacca gtccatgacc cgatcggctt tggagaagaa
     1261 gcgatggccg ccaatgtcga tgcgattgcc attgtggcaa atggtgcagg agataccgcc
     1321 gatgcgctgg gtagcctcaa gcacgatggg acggacgccg gagcggcgca ggaattcaag
     1381 ggccgcggtg agaccagcgg ggccggctcc aatgatgaca gcggttttca tgcagcaggg
     1441 attggatgcg aacgaaggaa gccgggcgaa tgaaggacga ggcctggcca aagcgacgca
     1501 ctgagaggag tttccatcac ggtcattgct tctatgttct gtgccgcgat gtgccctggg
     1561 caacagaaag ttcgaaaagg tgatagcaag tcgggcacct ggaacgagga ctagctggga
     1621 ttaacgattt ttccggcgcc tccgcaagcc tcgcattctt cgtagagagg caggttgcca
     1681 ttccagccga gggtcttgaa gccaactccg ttggcgttga ggctgattcc gctggcggcg
     1741 caggccggac agagaccatg ggcccggcaa aagtcctgcc aggcggaagg acggccggct
     1801 tcggtttcct tcgctcgcca ttctcgtgcc tcctgccagc gcttctgagc ggacagatag
     1861 cgggtgagga actcgccggt gaaagagacg cctcgcgcca tcagaaaatg agccctcgcg
     1921 aagttatcag gttaactctt tacggttggg gacagagtgt tttctgcaac tgaggacggg
     1981 gaggcgccgg aaagggtttc ccacccgcgc ctggagaggt cccaccaaat gagccagccg
     2041 gcaatggcgg cggcggcgag cagaacgaaa gccgcgagcc atgcgccgaa gatcagttta
     2101 ccgatgccga agaggcagcc ataaacaagg atgcagccaa gaatggtgtc gaatgcgttg
     2161 gcgcggaaat cgcgaaccgg cgggagttcg ggagcctgac tggcaatatg tttccatcca
     2221 tgcaccatgg ggtggacgcg cctgtagaag gcgagcagcg tcgcatcagg ctcggcgcga
     2281 gtgaggaagg tggcggcgat ccacgctacg gtcgtgacgc cggcggtgat gagtgtgctt
     2341 tttgcgaaga tgacggcatc gttgccggag aagtgaacgc gtgagaggct gagggcaacg
     2401 acggcagcaa cgatcatggc ggagatttcg ctccacgcgt tgatacgcca ccagaaccag
     2461 cgcaggatgt aaaccgcgcc ggtgccggcg ccgagattga gcaccagttc ccatccggcg
     2521 ccgatggagg tcagccggct ggcgacgtac ccgctgacga ggaccaggat cacggtgatg
     2581 atcttgctga cgaagacgta gtggcgttca ttggcgtccc gcaccatgaa gcggcgatag
     2641 agatcgctga ccaggtagga ggttccccag ttgagctgcg tgccgatggt cgacatgtag
     2701 gcagcgagga aggcggcgat catgaggcca cgcagagcgg gcggaagaaa atcgcgcagg
     2761 accatgacgt atccctgctg gggattggca ctgtaggcgg cgctgggatg gagaccgcca
     2821 tgggtggagt acatggcgag cgcaacgagg ccggtgacga tccacggcca cgggcggagc
     2881 gcatagtggg cgatgttgaa ccagagggtc gcgccgaggg agtgtttttc gttttttgcg
     2941 ctgaacatgc gctgggcgat gtagccaccc ccgccgggct cagcgccggg ataccaggcg
     3001 gcccaccact ggacgccgat gtacatgagg aaggtgagga gaggaagagt ccagaggttg
     3061 gaggtggtga ggcctgagtg gaagtcgggc aggaaggaaa ggacgctgga ggttggatgt
     3121 cctgcggcgc gggtagcggc gtcgacgatg ttcaggcgca cgcggagggc agacatgccg
     3181 ccaaggcgaa cgacggcgat ccaggcgagg acgatgatca tcgccatctt gagtacgaat
     3241 tgaaataaat cggtgacgag gacgccccag agtccgccaa gaaaggtata gaggccggtg
     3301 aagggaacca ggatgaagat gcagataaag agcgcggt
//
LOCUS       DASZYN010000624         7329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_610337, whole genome shotgun sequence.
ACCESSION   DASZYN010000624 DASZYN010000000
VERSION     DASZYN010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7329
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610337"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VHE33_16005"
     CDS             <1..945
                     /locus_tag="VHE33_16005"
                     /inference="COORDINATES: protein motif:HMM:NF015922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HVT99009.1"
                     /translation="GYLKRTPISTYRQQRRGGTGRLGMKTREEDFVTQLIVDSTHAYL
                     LCFTNKGRVYWLKVYEIPDVGAAGKGKSIASLLSLQPGETVRNILPVRNLEEEGKYIF
                     FATRKGTVKKTPLKDFCNVMARGIIAIGIDEDDELVAVRLTDGQQIVFLATHDGLAIR
                     FDEDGVRSMGRPAYGVRGIDIGKNDYVVGMAVTPKKNGSVHRILSVTESGFGKRTDVE
                     EYRLQSRGGKGVINVKTTARNGKVVGIQLVDETSEIIVISQYGKIIRIDTNTVRAAGR
                     STQGVKLLNLEEGDRVAAAVVIPPEEAKAEPETGTLLQ"
     gene            complement(1099..1644)
                     /locus_tag="VHE33_16010"
     CDS             complement(1099..1644)
                     /locus_tag="VHE33_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99010.1"
                     /translation="MGCWILADDPIGPLRTAQVFWHLDQYPGRAQAEADKGPRGVVIE
                     SLGKVWLMTIEDEKWRPAGGKRIGQIGPLPVTPGQKYSAQYMEAVFRPGMTAAQHLHS
                     GPEAWFTLAGETCLETSDGRVQVGRPGGSPVIVPAGLAMHLTATGTVERRALVLILHD
                     SSKPPTTMVHDWTPKGLCRGK"
     gene            complement(1941..3146)
                     /locus_tag="VHE33_16015"
     CDS             complement(1941..3146)
                     /locus_tag="VHE33_16015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT99011.1"
                     /translation="MASRSRSNRSPAGRVALRFVLMIGVVSFFADFVYEGGRSIGGPF
                     LETLGATGAIVGVVAGLGELLGYGLRFFSGRVSERTRQFWPMTLFGYFVQMAAVPSLA
                     LARNWQTAAALIVLERVGKAIRNPPRDVMLSHAGKEMGIGWAFGLHEALDQAGALLGP
                     LMLALILAYRGEYRLGFAALVIPAVITCVLIVVTKFLYPRPEDLEPHSPEIHAGGMGR
                     SFWIYLVGAALVAAGFADYQLIAFHLQKHGVASAIWIPIFYSVAMATSGAGSLLFGRL
                     FDRFGVTVLIPLTFISIASAPLVFLGGFRWALVGAALWGLGMGVHESIIPAAVATMVP
                     IERRPSAYGLFTAAYGIAWFLGSAAVGFLYDVSVPAAVVFCVATGMASLPFFFAVRNR
                     AQGAEHRAQ"
     gene            3405..3692
                     /locus_tag="VHE33_16020"
     CDS             3405..3692
                     /locus_tag="VHE33_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99012.1"
                     /translation="MKTMMVAAAVSGLLGGVTMMHASTTAANSGVTPILKAGKMSFAG
                     TDFAKPAKHSCKGKNDCKGQGGCKSSDNGCKGKNSCKGKGGCATDGSKMPD"
     gene            3761..4675
                     /locus_tag="VHE33_16025"
     CDS             3761..4675
                     /locus_tag="VHE33_16025"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HVT99013.1"
                     /translation="MPANRFNGFTDYGVGIGLRIPHYQHILSRHPVVDWFEIISENYM
                     VDGGRPLEVLDQILEQYKVVQHGVSMYFGSADPLSREHLKRLRSLVRRTKTPWLSDHL
                     CWGSVDGRYTHDLLPMPYTWEAVAVTAQKIRQVQDYCEVPVVVENVSSYAEFHASEMT
                     EWEFLNEVVERADCGILLDVNNIYVSSKNHNFDPFTYVNSVPSQRVAQIHIAGHSKYE
                     KYILDTHDHPVLPAVWRLYGRAIERCGPTATLLEWDDNIPSFDEVHAEALKANKFLKA
                     AAEAAPQQNSLAAMQAVAAVVEPAGAAR"
     gene            4672..5568
                     /locus_tag="VHE33_16030"
     CDS             4672..5568
                     /locus_tag="VHE33_16030"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HVT99014.1"
                     /translation="MSRSAGAPTGLLEIQRRMAAAVMHPLTRGETMPRKRRDGTSNRS
                     EADAIIRPNDRLTSFERLEIYNRQYWFRLYSSFEEDFPGLQAILGRARFDALMRAYLT
                     DCPSESFTLRNLGSRLEAWLGEHPDHLGPHPQLAMDMVRLEWAHIEAFDSEERPRLGA
                     KKFAAIDEHSTLRLQPHLRVLALSYPVDDLLLQVRTENGSAASSANNASVARRRRHIR
                     HIAGMAPKAIHLAIHRHENTVWYKELSPEEYGLLAALIDGKTLGEAIDLALANSSLPE
                     EQRPAFLQEAFASWSMLGWFTS"
     gene            5601..6098
                     /locus_tag="VHE33_16035"
     CDS             5601..6098
                     /locus_tag="VHE33_16035"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HVT99015.1"
                     /translation="MSTPATPTASDRPMGLIPALYRRFQLASARLESPFLLLVRVYWG
                     WQFAQSGWGRLHNVDRATGFFASLNIPAPHFTVICISLLEFIGGIMLIAGLASRFFGL
                     LLAGDMIVAYLLSDRDALAAIISDPGKFYNADPYTFLFASLMVLIFGAGRFSVDYYIF
                     RRKGC"
     gene            6175..7296
                     /locus_tag="VHE33_16040"
     CDS             6175..7296
                     /locus_tag="VHE33_16040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVT99016.1"
                     /translation="MKISARTRSFLPAIALALCAFHAPAQSTGQNPAPAATPEPAAAP
                     ASPISLPVTVVDKKGDPVKDLKAGDVTLTDNGHIQTIQSFALAQPSPMTFGVIGQISP
                     NQRTEIGDVRLATVHFLDHTLPGSDDRAFLIQYDRQVDLLEDPTATTNKLHDAVTQLG
                     SRQFGDQNGNSDESQDERRVGNWGGTLYDAIYLASSEVIAKEPGHHILVLIGDGIDRD
                     SKVSLGDAVAAAQAAHAAIFAIYFEGDKERPDLNRSGERRGGIGGNYPGTGGGYPGGG
                     GRRGGDREPAPQEPPHADGKETLEHLCNATGGYMVEGKRDKAEDAYNKLSALLKYQYT
                     LTWVPDQNASQSKTHHVTLNVTKKNDVWAIVQQDFSIAQ"
BASE COUNT         1491 a         2354 c         2222 g         1262 t
ORIGIN      
        1 ggctatctca agcggacgcc gatttccacg tatcgccagc agcggcgcgg gggcacgggc
       61 cggctcggca tgaagacgcg tgaggaggat ttcgtcacgc agctcatcgt cgactcgacg
      121 catgcttacc tgctctgctt caccaacaag ggccgggtct actggctgaa ggtctacgag
      181 attcccgacg taggcgcggc gggcaagggc aagtcgatcg ccagcctgct gagcctgcag
      241 ccgggcgaaa ccgtccgcaa cattctgcct gtccgcaacc ttgaagaaga gggcaagtac
      301 atcttcttcg ccacgcgcaa gggcacggtg aagaagacgc cgctgaaaga cttctgcaac
      361 gtgatggcgc gcggcatcat cgcgatcggc atcgacgaag acgacgagct ggtggccgtt
      421 cgcctgactg acgggcaaca gatcgtcttc ctcgccaccc acgacggcct ggcgatccgc
      481 ttcgacgagg acggtgtccg ctccatgggg cgtcccgcgt atggtgtccg cggcatcgat
      541 atcggcaaga acgattacgt ggtcggcatg gccgtgacgc cgaagaagaa cggctccgtg
      601 caccgcattt tgtcggttac ggagagcggc ttcggcaagc ggaccgacgt cgaggagtac
      661 cggctgcagt cgcgcggggg caagggcgtg atcaacgtca agaccacagc aaggaacggc
      721 aaggtggtcg gcatccagct ggtcgatgag acgtcggaga tcatcgtgat cagccagtac
      781 ggcaagatca tccgcatcga cacgaacacg gttcgcgctg cgggccgctc cacgcagggc
      841 gtcaagctgc tcaacctgga ggaaggcgat cgtgtcgcgg cggcggtggt gattccgccg
      901 gaagaggcga aggcggagcc ggagaccggg acgttgctgc agtaagaaca gggctcaggg
      961 tgcagggagc ggcggcttag gccgccgctt cgttttgttc gaacgtaccg attggaatct
     1021 cgtgcagatc cggagtccaa tcgctgtccc caacgtgatc cctgcggggg cacgaacgag
     1081 ctgaacatcg gctgccgttc acttgcccct gcacaatccc tttggcgtcc agtcatgcac
     1141 catggtcgtg ggcggtttgg atgagtcgtg aaggatgagg acgagagcac ggcgctcgac
     1201 cgtcccggtg gccgtaaggt gcatcgctaa accagcgggg acaatgacgg gcgagccacc
     1261 gggtcgtccg acctgcacgc gaccatcgga cgtttcgagg cacgtttccc ccgccagtgt
     1321 gaaccacgcc tccggaccgg aatgaagatg ttgagccgcg gtcattcctg gcctgaaaac
     1381 cgcttccata tattgggcgg aatacttctg gcccggggtc acggggagag gaccaatttg
     1441 gccgatgcgc tttccgccag cgggcctcca tttctcatct tcgatggtca ttagccacac
     1501 cttgccgaga gattcgatga ccaccccacg cggccctttg tccgcctcgg cctgggcgcg
     1561 gcccggatac tggtcgagat gccaaaatac ctgcgcagtt ctgagggggc caattggatc
     1621 gtccgcgaga atccagcatc ccacgtcctg agtccgctgg ctcgccggct tgcaggcatc
     1681 cccctttacc gcaacgccct gtccgcgcat tggagcgcac aaaaacccga ggaaaagaca
     1741 agcaacctgg gccacgaccc gactgtgtcg aaacacctga cgcacctctt tccgtacgct
     1801 gtttcagcca gggatttaac tatcggctga catcattcca ttttctgcaa ttgtctgtcg
     1861 aggaatgcgg tttctccacg gaccccaacc ggtcgctgga ccctgtctcc gtgcgcctcc
     1921 ccagggccaa aggcacgccc ttactgtgct cggtgttccg cgccctgcgc ccggttcctg
     1981 accgcaaaga agaacggcag tgaagccatt cccgtcgcga cgcaaaagac taccgctgcc
     2041 ggaaccgaaa cgtcgtacaa aaagccgacg gccgcgctgc ccaggaacca ggcgatgccg
     2101 taagccgcag tgaacagtcc atacgccgaa ggacgccgct cgatgggcac catggtcgcg
     2161 accgcggcgg ggatgatcga ttcatgcacg cccatgccca gaccccagag cgcggcgccc
     2221 accagggccc agcggaatcc gccaagaaaa acgagcggcg ccgatgcgat ggagatgaat
     2281 gtgaggggga tgaggaccgt caccccaaaa cggtcaaaca gccgcccaaa cagcagcgac
     2341 cccgcgccgc tggtcgccat ggcgaccgag tagaaaatag gaatccagat cgccgaggcg
     2401 acaccgtgct tctgcagatg gaaggcgatc aattgataat cggcgaagcc ggccgcgacc
     2461 agcgccgcgc cgacgagata gatccagaag gaacgcccca tgcccccggc gtgaatttcc
     2521 ggcgagtgcg gttccagatc ctcgggccgc ggatacagga atttcgtaac aacaatgagc
     2581 acacaagtga tgacagccgg aatcacgagc gccgcaaatc caagccgata ctcgccgcga
     2641 taagcgagga tgagcgccag catcagcggg ccgagcagcg caccggcctg gtccagcgcc
     2701 tcatgaagcc cgaaagccca gccaatgccc atttctttgc cggcgtgcga gagcatcaca
     2761 tcgcgcggcg gattgcgaat ggctttgccg acccgctcca gcacgatgag cgccgcggcc
     2821 gtctgccagt tgcgcgccag cgcgagcgag ggcaccgcgg ccatttggac aaaatagccg
     2881 aagagcgtca tcggccagaa ctggcgggtc ctctcgctga cgcgcccgga gaagaaccgc
     2941 aacccgtacc cgagcagttc gcccaggccg gccaccacgc caacgatcgc acccgtagca
     3001 cccagggttt cgaggaacgg ccccccgatg ctgcgcccgc cctcgtagac gaaatcggca
     3061 aagaagctga cgacgccgat catcaggaca aagcggagcg ccaccctgcc tgccggcgac
     3121 cggttcgatc tgctacgtga cgccatttcg tccctgagtc attcctgtca ttggagatgg
     3181 gttgcggcga agaattgtct cgaaattgtc tccgcctggc tgaagtgaac gttcggtgaa
     3241 tgagtggaaa aaaacaatcc ggcagggtgg cacaacgcgt agttgtttat gtagtctaat
     3301 ccgcacccgc ggggttcccc cgcctggcgt tccccagttg ttcccaggtt gcgccggtct
     3361 gggcctcgca atccgatgga ggaatgacac aaatgaagca atcgatgaag accatgatgg
     3421 tggctgcggc cgtatccggt ctgctgggcg gcgtgacaat gatgcacgcg tcgaccacag
     3481 ccgcgaattc gggcgttacg ccgatcctca aggcgggcaa gatgtcgttc gccggcaccg
     3541 atttcgcaaa acccgcgaag cactcgtgta aaggcaaaaa cgactgcaaa ggccagggcg
     3601 gctgcaagag cagcgacaat ggctgcaaag gcaagaacag ctgcaagggc aagggcggct
     3661 gcgcaaccga cggctccaag atgcccgact agttttaccc cggcacggaa gggccgcctc
     3721 aaccgcggcc cttcttgctt tcacccctcg aggttcccca gtgcctgcaa atcgcttcaa
     3781 cggattcacg gactacggcg tcggcatcgg tctgcgcatc ccgcattatc agcacattct
     3841 ttcgcgccat ccggtcgttg actggttcga gatcatctcg gaaaactaca tggtcgacgg
     3901 cggccggccg ctggaagtgc tcgaccagat cctcgagcag tacaaggtcg tgcagcacgg
     3961 cgtttcgatg tacttcggct cggcggatcc gctcagccgt gaacacctca agaggctcag
     4021 gagcctcgtc cggcgcacca aaacgccctg gctcagcgat catctctgct ggggatcggt
     4081 cgacggccgc tacacgcacg acctgctgcc catgccgtac acctgggaag ccgtcgcggt
     4141 cacagcgcaa aagattcgcc aggtgcagga ctactgtgaa gtgccggtgg tcgttgaaaa
     4201 tgtctccagc tatgcagagt ttcatgcttc ggagatgacg gagtgggaat tcctgaacga
     4261 agtggtcgag cgcgccgact gcggcatcct gctcgacgtg aacaacattt atgtgtcgtc
     4321 gaagaaccac aacttcgatc cgttcaccta tgtgaactcc gtcccctcgc agcgcgtcgc
     4381 gcagattcac atcgccggtc acagcaaata cgagaagtac atcctcgaca cacacgatca
     4441 tcccgtgctg ccggcggtgt ggcgccttta cgggcgcgcc attgagcgct gcggaccgac
     4501 ggccacgctg ctggaatggg acgacaatat cccgagcttc gacgaagttc acgcggaggc
     4561 gctcaaggcc aacaagttcc tcaaggccgc cgctgaagca gcgccgcaac agaacagtct
     4621 tgcagcgatg caggctgtgg cagcggtcgt cgaacccgcc ggagccgcac gatgagcaga
     4681 tctgccggcg caccgaccgg gctgctggag atccagcgcc gcatggccgc tgccgtgatg
     4741 catccgctga cgcgcggcga gaccatgccg cgcaaacgcc gtgacggcac ctcgaaccgg
     4801 agcgaagccg acgcgatcat ccggcccaac gaccggttga cttcgttcga gcgcctggag
     4861 atttacaacc gccagtactg gttccgtctc tactcctcat tcgaagaaga tttccccggt
     4921 ctgcaggcga tcctgggccg tgcgcgcttt gacgctctga tgcgggccta ccttaccgac
     4981 tgcccgtcgg agtcgttcac gctgcgcaac ctgggttcgc gcctcgaagc gtggctggga
     5041 gagcacccgg accacctggg cccgcatccg cagcttgcta tggacatggt gcggctggaa
     5101 tgggcgcaca tcgaagcctt cgactcggaa gagcgtccgc ggctcggcgc gaagaaattc
     5161 gcggccatcg acgagcattc gactctgcgt ctgcagccgc atctgcgcgt gctcgcgctg
     5221 tcgtacccgg tggacgattt gctgctgcag gtccgcaccg agaacggatc ggcggcttca
     5281 tcggccaaca atgccagcgt cgcacgcagg cgacgccaca ttcggcacat tgccggaatg
     5341 gcgcccaaag cgatccacct ggcgatccat cggcacgaga atacggtctg gtacaaggag
     5401 ctttctccgg aagagtacgg tttgctggcg gcgctgatcg acggcaaaac gctgggcgag
     5461 gcgatcgacc tcgcgctcgc caacagcagc ttgcccgaag agcaacggcc ggcatttctg
     5521 caggaagcgt tcgccagctg gtctatgctg gggtggttca cttcctgacc acgcttcctc
     5581 cccacgaaag gcacgactcg atgagcaccc ctgcgacccc aaccgcgtcc gaccgcccca
     5641 tgggcttgat tcccgcgctg tatcgccgct tccagctcgc ctcggccagg ctggagtcgc
     5701 ctttcctgct gctcgtccgc gtctactggg gatggcaatt cgcgcaatcc ggctggggac
     5761 ggctgcacaa cgtggatcgc gccactggtt tcttcgccag cctcaacatt cccgcgcccc
     5821 acttcaccgt catctgcatt tctttgctgg agttcattgg cggaatcatg ctgattgccg
     5881 gattagccag ccgtttcttc gggctgctgc tggccggcga catgatcgtt gcctatctct
     5941 tgtccgatcg cgatgcgctg gcggccatca tcagcgaccc gggcaagttc tacaacgccg
     6001 atccctacac cttcctcttt gcttccctca tggtgctcat cttcggcgcc gggcgctttt
     6061 ccgtggacta ctacatcttt cgccgcaagg gctgctgagg ggctcgatcc agcagcgaaa
     6121 cgtcgggttc ggtgctaacg tctaatgggt ggtccacacc actcccgacc cgccatgaag
     6181 atttccgccc gcacccggtc tttcctgccc gcaatcgccc tggcgctgtg cgccttccac
     6241 gctccggctc aatcgaccgg ccagaatcct gcaccggctg cgactcccga accggccgcg
     6301 gcgccggcca gccccatcag cctgcccgtc actgtcgtcg ataaaaaagg cgatcccgtg
     6361 aaggacctca aggccggcga cgtcacgctg accgacaacg gccatatcca gaccatccag
     6421 tccttcgcgc tggcgcagcc ttcgcccatg actttcggcg tcatcgggca gatcagcccg
     6481 aaccagcgca cggagatcgg cgatgtccgc ctcgcaacgg tgcacttcct tgatcacact
     6541 ctgccgggca gcgatgacag ggcttttctg atccagtacg accgccaggt cgacctgctg
     6601 gaggatccga cggctacaac caataagctg cacgatgcgg tcacgcagct gggatcgcgg
     6661 caatttggcg atcagaacgg caactccgac gagagccagg atgaacgccg tgtcggcaac
     6721 tggggtggca cgctttacga cgcaatctac ctggcatcga gcgaagtcat cgcgaaggaa
     6781 cccggccatc acatcctcgt gctgatcggc gacggcatcg atcgcgacag caaggtgagc
     6841 ctcggcgatg ccgtggcagc cgcacaggcg gcccatgcgg cgatcttcgc gatttacttc
     6901 gagggcgaca aggagcggcc cgaccttaac cggagcggcg aacgccgcgg cgggatcggc
     6961 ggcaattatc ccggcaccgg aggcggatat ccagggggcg gcgggcggcg cggcggcgat
     7021 cgcgaaccgg ctccgcagga gcctccgcat gccgacggca aagagacgct ggagcacctg
     7081 tgcaacgcca ccggcggata catggtggag ggcaagcggg acaaggcaga agacgcctac
     7141 aacaagctga gtgcgttgct gaagtatcag tacacgctga cctgggtccc ggatcagaac
     7201 gcgtcgcagt cgaagacgca tcacgtgacc ctcaacgtca cgaagaagaa cgacgtgtgg
     7261 gcgatcgtcc agcaggattt ctcaatagcg cagtagaata ggaattccct tcaggtgctt
     7321 tctccagtc
//
LOCUS       DASZYN010000625         3206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_288161, whole genome shotgun sequence.
ACCESSION   DASZYN010000625 DASZYN010000000
VERSION     DASZYN010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288161"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VHE33_16045"
     CDS             <1..744
                     /locus_tag="VHE33_16045"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HVT99017.1"
                     /translation="VGYSQTQTFATISQQAETVFNHYVSPNAVIGDARSELTEFIRTK
                     EFNQNTMLASRQMVEEIQNEVRMYKSLNGVPANQQQNVRNDMYVVSEALRLIAKYPGK
                     NGAPTFTAAENKTLSAYKKQLDNATKFIPTWVKVAVAIALGLGTMVGWKRIVVTVGEK
                     IGKEHLTYAQGAAAEIVAAVTIIAADQYHMPVSTTHVLSSGVAGTMLANGSGLQLSTI
                     RNIALAWIFTLPAAALLAAGIFLVLRQFM"
     gene            917..2038
                     /gene="scpB"
                     /locus_tag="VHE33_16050"
     CDS             917..2038
                     /gene="scpB"
                     /locus_tag="VHE33_16050"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HVT99018.1"
                     /translation="MSLKARIEAVIYAAEEPVTLAQLASLFRDEALALRAARSNASAD
                     PEAPAESDVPMVSDLVPTGKEAPRPSEDSAQNSADAEVATVDFAGDPQPAPDPPGTAV
                     NQIAPEQEASDESVSLEAAAEAEKKAARQRDREVRDEIRRVIDELIDDYNAADSGLEI
                     REVAGGYRFGTRPEYHDAVRAFIKTLRPPFKLSMQALETLAVIAYKQPITAPEIGEIR
                     GVDSSGVLGSLVARKLITTAGRKQVLGRPILYKTTKEFLVRFGLKDVNELPSLEEFEK
                     MASELAETEAEGAIAPGEEELPFAQNEMAEIEEASSSDVEANSVSARDPHDPNAPAPP
                     MNDPEPEEPEPPISDPPAEPEPAPTTEMDAAEHAEKAHR"
     gene            2035..3018
                     /locus_tag="VHE33_16055"
     CDS             2035..3018
                     /locus_tag="VHE33_16055"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HVT99019.1"
                     /translation="MTEPGETSEDAASGTGQRLQKIIAAAGVASRRKAEELILAGRVQ
                     VNGQTVTTLGAKADSTRDHIRVDGKLLHGPERHRYVVLNKPKGYVTTASDPEGRPTVM
                     NLVGTGKTAGGERLFPVGRLDYTSEGLLLMTNDGDLANALTRAAARVEKTYLVKVSGK
                     PSDSALNQLRHGVMIDRGSQIQGPRPDSRKPRRAPRSDQPRHNEGRVMTAPARIRMLR
                     DAENPWYELILIEGRNREIRKMFEEVGHHVEKIRRVGYGPLVLDLEPGRSRELTLDEV
                     SALRRAAEGKRSKPALEVRLPKAGRRPRTRAASSEGRKPSGRTRIPPSKSR"
     gene            3064..>3206
                     /locus_tag="VHE33_16060"
     CDS             3064..>3206
                     /locus_tag="VHE33_16060"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HVT99020.1"
                     /translation="MEKATFAAGCFWGVEARFQQLPGVTATAVGYEGGSLASPTYQQV
                     CTDR"
BASE COUNT          680 a         1101 c          914 g          511 t
ORIGIN      
        1 gtgggataca gccagaccca aaccttcgcc acgatttcac agcaggcaga gaccgtcttc
       61 aaccactacg tctcgcccaa cgccgtcatc ggcgatgctc gcagcgagct tacggaattc
      121 atccgcacga aagaattcaa ccagaacacc atgctggcct cccgccagat ggtcgaggaa
      181 attcagaacg aggtcaggat gtacaaatcg ctcaatggcg ttccggcgaa ccagcagcag
      241 aacgtccgca acgacatgta cgtcgtgagc gaggctttac gactgatcgc caaatacccc
      301 ggcaagaacg gtgcgcccac cttcactgcc gcagagaaca agactctgag cgcctacaaa
      361 aagcaactcg ataatgccac aaaatttatc ccgacctggg tcaaagtcgc cgtagctatc
      421 gctctcggcc tgggcacgat ggtcggctgg aagcgcatcg ttgtcaccgt cggcgaaaag
      481 atcggcaagg agcacctgac ctacgcacag ggcgctgccg ctgagatcgt cgccgccgtc
      541 actatcattg ccgccgatca gtaccatatg ccggtttcca caacccacgt gctgtcctcg
      601 ggcgtggccg gcaccatgtt ggccaatggc agcggcctcc agctctccac catccgcaac
      661 atcgcgctgg cctggatttt cactctcccc gccgcggcat tgctcgccgc tggtatcttc
      721 ctcgtgctgc gccagttcat gtgatccaca gccctttcgc accagaggcg gcgctttcgg
      781 tagtatcgag aaagcgccgc ttttctttgc gcgagttcgt tcttgtatca gggcacgagt
      841 tcactcgtgt cgttaaccgc ccaaattgga ttgggcttta gcccccgagg aaagccgttt
      901 tcgtccccac ccaccgatga gtctcaaagc ccgcatcgaa gccgtcattt acgccgccga
      961 agagcccgta accctggctc agcttgcctc cctcttccgt gatgaagccc ttgccctccg
     1021 cgccgcccgc agcaatgcgt ccgcggatcc ggaagccccg gcggaatccg acgtacccat
     1081 ggtttcggac ctcgtgccca ctggcaaaga agctccacgt ccgtcagaag actcggcgca
     1141 aaactccgcc gacgccgaag ttgcgacggt cgattttgcc ggcgatccgc agccggcgcc
     1201 cgaccccccg gggacagcgg tgaaccagat cgcccctgaa caggaagctt cggatgaaag
     1261 cgtctccctc gaagctgcgg cagaggccga gaagaaagcg gcccgccagc gcgatcgcga
     1321 agtccgcgac gaaatacgcc gcgttatcga cgagctcatc gacgattaca acgcggccga
     1381 cagcggcctc gagattcgag aagtcgcggg tggctaccgt ttcggcaccc gtcccgagta
     1441 tcacgatgcg gtccgcgcct ttatcaagac cctcaggccg cccttcaaac tctccatgca
     1501 ggcgctcgaa accctggccg tgatcgccta caagcagccc atcaccgcgc ctgaaatcgg
     1561 tgagattcgc ggcgtcgact cgagcggcgt gctcggttcc cttgttgccc gcaagctgat
     1621 caccaccgcc gggcgcaaac aggttcttgg ccgccccatt ctctacaaga ccacgaaaga
     1681 gttcctcgtc cgcttcggcc tcaaggatgt caacgagctg ccctccctgg aggagttcga
     1741 gaagatggcc agcgaactgg cagaaaccga agccgaaggc gcgatcgcgc caggggaaga
     1801 ggagttaccg ttcgctcaga acgaaatggc cgaaatcgag gaggcctctt cttcggacgt
     1861 agaagccaac tcagtctccg ctcgggaccc gcacgaccca aatgctcccg ccccgcccat
     1921 gaacgatccg gaaccggaag agccagaacc gcccatcagc gaccctccag ccgagcctga
     1981 accggcgcct accacggaaa tggacgcggc tgaacacgcg gaaaaggcac accgatgacc
     2041 gagcccggcg agacgagcga ggatgcggcg tccggcacgg gacagcgtct gcagaagatc
     2101 atcgcggcgg ccggggtggc gtcccggcgt aaagccgaag agctgatcct cgctggacgc
     2161 gtgcaggtca atggtcaaac cgtcaccacc ctcggcgcca aagccgattc cacgcgcgac
     2221 cacatccggg tagatggcaa gctcctgcac ggaccggaac gacaccgcta cgtcgtcctg
     2281 aacaaaccaa aagggtatgt aaccaccgcc agcgacccgg aaggccgccc caccgtcatg
     2341 aacctggtcg gaaccggaaa gacggcgggg ggcgagcgcc tcttcccggt aggccgtctc
     2401 gactacacca gcgaaggcct cctgctgatg accaacgacg gcgacctcgc caacgcgttg
     2461 acacgcgcgg cggcccgcgt cgagaagacc tacctcgtca aagtcagcgg aaagccgtcg
     2521 gactcggcgc tgaatcagct gcgccacggg gtcatgattg accgcggctc gcaaattcaa
     2581 ggcccgcgtc cggattcccg caagccccgg cgcgccccac gctccgacca gcctcgccac
     2641 aacgaaggcc gagtcatgac cgccccggcg cgcatccgta tgctgcgcga cgccgagaat
     2701 ccctggtacg agctgattct catcgaaggc cgcaaccgtg agatccgcaa gatgttcgag
     2761 gaagtcggcc accatgtcga aaagattcgc cgtgtgggct atggtccgct cgtgctggat
     2821 ctcgagcccg gccggtcccg cgaactcacc ctcgacgagg tctcggctct gcgcagggcg
     2881 gcggagggta agcgttcaaa acccgcgttg gaggtccggc tccccaaggc cggacgccgt
     2941 ccccggactc gggcggcgag ttcggaaggg cgcaagcctt caggccgcac caggatcccg
     3001 ccttccaaaa gtcgctgaaa ccccagtccc tgtcgcacaa tctaatggga ggagatcaat
     3061 cccatggaaa aagccacttt tgccgccgga tgcttctggg gcgtcgaagc ccgcttccag
     3121 caactgcccg gcgtgacggc gaccgccgtc ggctacgagg gcggctcgct cgccagcccc
     3181 acctatcagc aggtttgcac cgaccg
//
LOCUS       DASZYN010000626         1788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_610664, whole genome shotgun sequence.
ACCESSION   DASZYN010000626 DASZYN010000000
VERSION     DASZYN010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610664"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..636)
                     /locus_tag="VHE33_16065"
     CDS             complement(247..636)
                     /locus_tag="VHE33_16065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT99021.1"
                     /translation="MPTVLVVDDEHGILVTLSAILKGHGFTVFTARSGEEAVDVAYNV
                     EPDWVVSDVVMGTLSGIDAAIRIRDVCPDTQVILFSGNALTTDLLAEARSHGHHFEIL
                     AKPFDPAELVKRLSPPAKQHSTEEIAC"
     gene            777..1184
                     /locus_tag="VHE33_16070"
     CDS             777..1184
                     /locus_tag="VHE33_16070"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HVT99022.1"
                     /translation="MVSIEFEYQGDLHCRAVHGPSGTVLNTDAPKDNQGRGESFSPTD
                     LIATALGSCMLTTMGILARRLEVNLTGATATVEKEMASAPFRTIGQLTVRIRIPREVS
                     PEDRAKLENAAHMCPVAKSLSPEVQIPTTFTWG"
     gene            1367..>1788
                     /locus_tag="VHE33_16075"
     CDS             1367..>1788
                     /locus_tag="VHE33_16075"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVT99023.1"
                     /translation="MIAEATNPDPAFSFAFQPIVDTVAHKVIAYEALIRGPWDEPAWQ
                     VLNQVSPLRLHVFDQTARSAAIALAGRLNLEHPLHLNFLPQTLDASPKSLLATVDAAR
                     QAGIAPDRLVLEVPESEVVVERHRFAAILREVRSLGVRL"
BASE COUNT          356 a          577 c          496 g          359 t
ORIGIN      
        1 gacggatcga ttttgacttc agcactgagg acagaaagac cagcgacaag gttgcggaaa
       61 tcctgagaag ccatcgggag aaaagccttg cctcgatcgg caccatcgag cttcctgcgg
      121 atctattagc tgcgtaggaa aactatcttc tcccgatcca cggcgctgga caggagtgga
      181 agagctgagt ggccgccttc ctgaaggcga catcgattac tgacgtcgac acggatccgt
      241 gcacactcag cacgcgatct cttcggtgct atgttgtttc gcaggcgggc tgagccgctt
      301 cacgagttcc gcgggatcga atggtttagc gagtatctca aagtggtggc cgtgcgaccg
      361 cgcttccgcc agcaggtccg tggtcagcgc attgcctgaa aacagaatga cctgcgtgtc
      421 gggacaaacg tcgcgaatcc ggatcgccgc atcgatgccg ctcagcgtgc ccatcaccac
      481 atcggaaacg acccagtcgg gctcgacgtt gtaagcgaca tcgaccgctt cctcaccact
      541 gcgggcggtg aacacggtga atccgtgacc ctttagaatg gcggaaaggg tgaccagaat
      601 cccgtgctca tcatcgacca caagaacggt tggcataggt ttcgctaaca tagtgactat
      661 cctttaaagg agcaataccc gagagagttc tgctcttcga tgttgacgtt ttcgactaag
      721 ttgtatcaat aactacagta acctataact ctttcgagtt atctcagcga ggaagcatgg
      781 tatccatcga attcgaatat cagggcgatc tgcactgcag ggcggtccac ggcccttccg
      841 gcacggtact caacaccgac gcccccaaag acaatcaggg ccggggtgaa agcttctctc
      901 ctacggacct aatcgcgacc gccctcggca gctgcatgct taccaccatg ggcatccttg
      961 cccgccggct ggaggtgaac ctcaccgggg caacggccac ggtggagaaa gaaatggcca
     1021 gcgctccgtt ccggaccatc ggccagctta ccgttcgcat ccgcatcccc cgcgaggtga
     1081 gccccgaaga ccgggcaaaa ctggagaacg ccgctcacat gtgcccagtc gcaaaatctc
     1141 tcagccctga ggttcagatt cccaccactt tcacctgggg atgaacggag acgcactctc
     1201 ccctggcggg gttgtacccc gttcgcgctg tttactctcc cccattcgtg gactgcctca
     1261 tcttcccggc gcgcgtgacg ataagcgtac tgccgcgtag ttcctccccc accttttgcg
     1321 gccacgtggc ctgactgttg gcaaggcggc gtgccaactg tgcaccatga tcgccgaagc
     1381 gaccaatccg gatccggcgt tctctttcgc cttccagccc atcgtggaca cggtggcgca
     1441 caaggtcatc gcctacgaag cgctcatccg cggcccgtgg gacgagcccg cgtggcaggt
     1501 tctcaaccag gtttctccgc tgcgcctcca cgttttcgat cagacggccc gctctgcggc
     1561 catcgcactc gccggccggc tgaacctgga gcatccgctt cacctcaact tcctccccca
     1621 gaccctcgac gcttcaccga agtcgcttct tgccaccgtt gacgcggcgc ggcaggccgg
     1681 gatcgcgccc gaccgtctcg tgcttgaggt gccggagagc gaagtcgtgg tcgagcgcca
     1741 tcgctttgcc gcgattctgc gcgaggtccg ctccctcggg gttcgcct
//
LOCUS       DASZYN010000627         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_483021, whole genome shotgun sequence.
ACCESSION   DASZYN010000627 DASZYN010000000
VERSION     DASZYN010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483021"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..>1783)
                     /gene="kdpB"
                     /locus_tag="VHE33_16080"
     CDS             complement(142..>1783)
                     /gene="kdpB"
                     /locus_tag="VHE33_16080"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HVT99024.1"
                     /translation="VASVDESAITGESAPVIRESGGDRSAVTGGTRVLSDWIVVQITA
                     NPGETFLDRMISLVEGARRQKTPNEIALNILITSLTIIFVLVVVSLEPFAIYSGQAVP
                     VVTLIALLVCLIPTTIGGLLSAIGIAGMDRMVQRNVLAMSGRAVEAAGDVDALLLDKT
                     GTITIGNRMATRFVPIKGVEEREVAHAAMLSSLSDETPEGRSIVVLARERYGFTDEID
                     PALSFVPFSATTRMSGVDRLNGSNEPAQSIRKGAGDSIERYVQEQGGETSAELQEIVT
                     TIAKVGSTPLVVAETSINGSEKQARVLGVIELKDIVKSGMRERFEQMRRMGIRTIMIT
                     GDNPLTAAAIAREAGVDDFLAQATPETKMKLIKDQQQGGRLVAMTGDGTNDAPALAQA
                     DVGVAMNTGTQAAKEAANMVDLDSNPTKLIEVVEVGKQLLITRGALTTFSIANDVAKY
                     FAIIPAAFAVTYPQLNALNIMGLATPHSAVLSAVIFNALVIIALIPLALRGIRYRPIG
                     AGPLLRRNLLIYGLGGIIVPFLGIKLIDLILVALHLTL"
BASE COUNT          355 a          556 c          503 g          369 t
ORIGIN      
        1 cggtcaccat caccagcagc agcgtgatga ccactgccgg gcgtagatat ttctgcaaaa
       61 aagttttcat cttgcgtctt ctttcagaac gcgaggcgtt tccccagagg aagcgccttg
      121 ccattcacgc cactgcgttt cttacaaggt caggtgcaat gccaccagga tgagatcgat
      181 cagcttgatg ccaaggaacg ggacgatgat gccacccagc ccgtagatca gcagattacg
      241 ccgcagcagc ggacccgcac ctatcgggcg gtacctgatg ccgcgcaggg ccagtgggat
      301 gagggcgatg atcaccaggg cattaaagat caccgctgag agcaccgcgc tgtgcggggt
      361 cgccagaccc atgatgttga gcgcattcaa ctgcggatag gtcaccgcga aggccgccgg
      421 gataatggca aagtacttgg ccacgtcgtt ggcaatgcta aatgtcgtca gtgcgccgcg
      481 agtgattagc aactgcttgc caacctcgac gacctcgatc aatttggtcg gattggagtc
      541 taaatctacc atattggcgg cctctttggc ggcctgtgtg cccgtgttca tcgctacccc
      601 gacatctgcc tgcgccaggg ccggggcatc gttggtacca tcacccgtca tggcaaccag
      661 gcgaccaccc tgctgctgat ctttaatcag tttcatcttg gtttcaggag tggcctgggc
      721 caggaaatcg tctaccccgg cctctctggc aatcgccgcc gcggtcagtg ggttgtctcc
      781 tgtaatcatg atcgtgcgaa tgcccatgcg tctcatctgc tcgaagcgct cgcgcatccc
      841 gcttttgaca atatccttga gctcgattac ccccagcaca cgcgcctgct tttcgcttcc
      901 attgattgag gtttccgcca cgactagcgg cgtactcccg actttcgcga tggtcgtcac
      961 gatctcctgg agctccgcgc tggtctcgcc gccttgttcc tggacatagc gttcgatgga
     1021 atcgcccgcg cccttgcgta tactctgggc aggctcgttg ctgccattga ggcgatccac
     1081 gccgctcatg cgggtggttg cgctaaaggg tacaaagctc agcgcggggt caatttcgtc
     1141 cgtgaagccg taacgctcgc gggccagcac cacgattgag cggccctccg gcgtctcgtc
     1201 ggagagactg gaaagcatcg ccgcgtgtgc cacctcccgc tcctctactc ctttgattgg
     1261 cacaaagcga gtcgccatgc ggttgccgat ggtgatagtg ccagttttgt ccagcagcag
     1321 cgcatccaca tcgcccgccg cctcgaccgc gcgcccgctc atggccagca cattgcgctg
     1381 taccatgcgg tccatccccg cgatgccaat cgccgagagt agtccgccaa tagtggtggg
     1441 gatcaagcag accagaaggg cgatcagcgt gacgaccggg accgcctggc cagaatagat
     1501 ggcaaacggc tccagagaaa caaccaccag gacaaagatg atcgtcagac tggtgatcaa
     1561 gatattcagg gcgatctcat tcggcgtctt ctggcgcctg gccccttcga ccagcgagat
     1621 catacgatcc aggaaggttt cgcccgggtt agcagtgatc tggaccacaa tccagtccga
     1681 aagcacgcgt gtgccgccgg tgaccgcaga acgatctcca ccgctctcgc gaatcacggg
     1741 agcggattcc cctgtaatgg ccgactcatc gaccgaggcc acg
//
LOCUS       DASZYN010000628         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_734693, whole genome shotgun sequence.
ACCESSION   DASZYN010000628 DASZYN010000000
VERSION     DASZYN010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734693"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VHE33_16085"
     CDS             <1..377
                     /locus_tag="VHE33_16085"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HVT99025.1"
                     /translation="PETVNHRTIYKYVHLNGARSCQLVMGVTHLHTGSAWNTMPPHTH
                     MRRSEIYMYFNLPQDARVFHFMGPPEETRHLVLSDHDIVISPGWSIHAGVGTQAYSFC
                     WGMGGENQDYADMDPAPLDTLL"
     gene            414..905
                     /locus_tag="VHE33_16090"
     CDS             414..905
                     /locus_tag="VHE33_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99026.1"
                     /translation="MKTALLATAAILTLAVSASAQTPPSSAPHDPAVVFPTADLHDQI
                     EKLVPAAKEKGSSGATIEDYGSYKIQLSVRTSSGGAEVHAHWDDVMIVEKGSATLITG
                     GTVADSKTNAEGETHGSKIENGDSRTIKEGDVLTVRAGTPHQLILAPGTTYGAVVVKI
                     HEP"
     gene            complement(941..1816)
                     /locus_tag="VHE33_16095"
     CDS             complement(941..1816)
                     /locus_tag="VHE33_16095"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HVT99027.1"
                     /translation="MRQKKAEVQPERVEEKVAKRPSDPYQLQSLDRAVSVLDLLGESE
                     GPLGLADVCERMDLHKSTAHRALMVLERCGLIERTPENRFRLGLKLYELGTRAVEQID
                     LRARVHPWFRRLSAQVGETVHLGVLQKTSVVYLDKVEPNNRRVWLSSRIGTSNPVYCT
                     AMGKAMMAFLPEEMATEIMAKIRFVRYTHRTLMTPEALMRSLERVRRRGYAIDDEEVE
                     EGVRCIGAPILNEAGQPMAAVSVSGPTSRITQQSVPGIAEHLMRCCREISASLGVRER
                     RRTHAPSPFLQHYGL"
     gene            complement(1831..2595)
                     /locus_tag="VHE33_16100"
     CDS             complement(1831..2595)
                     /locus_tag="VHE33_16100"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HVT99028.1"
                     /translation="MGIAERFRLDGKTALVTGAASGLGAAMALGLADAGAAVACHGNR
                     RPAEETAQRIRDIGRDGQSFSADLGSDEGADKLFSEVAAAMGAPDILVNNAGMIHRAA
                     AADHDLDAWKQVLQVNLTSVFRLCQLVGAKMLERRQGKIINIASMMAFQGGIRIAAYT
                     ASKGAIAQLTKALANEWAPHNVQVNAIAPGYFRTENTSALQKDETRNRQILERIPAGR
                     WGDPEDLAGAAVFLSSAASDYVTGTVLVVDGGWLAR"
BASE COUNT          539 a          921 c          822 g          550 t
ORIGIN      
        1 cgccggaaac ggtcaaccac cggaccattt acaaatacgt tcacctgaac ggcgcgcgga
       61 gctgccaact ggtgatgggc gtgacgcacc tgcacacggg gagcgcatgg aacacgatgc
      121 ccccccacac gcacatgcgg cgctcggaaa tctacatgta tttcaacctg ccgcaggacg
      181 cccgcgtttt tcacttcatg gggccgccgg aggagacgcg gcatctggtg ctttcggacc
      241 acgacatcgt catctctccg ggctggtcga ttcacgccgg cgtcggtacc caggcttata
      301 gtttttgctg gggcatgggc ggcgagaatc aggactacgc cgatatggat cctgcgccgc
      361 tcgacacact gctgtagcct cgttcgcgaa gttcattctg caaaggagcc ccgatgaaga
      421 ccgctctcct cgctacggcc gccattctga cacttgccgt ttccgcgtcc gcgcaaactc
      481 ctccttcgag cgcaccgcac gatcccgccg ttgttttccc aactgccgat ctgcacgacc
      541 agattgaaaa gctcgtccct gcggcaaaag agaagggatc cagcggagcg acgatcgaag
      601 actacggcag ttacaagatt cagctctcgg tgcgaacttc gagtggcgga gccgaggtcc
      661 acgcgcactg ggacgacgtg atgatcgtgg agaaaggcag cgccaccctc atcaccgggg
      721 gaaccgtggc cgattccaaa acgaacgccg agggcgagac gcacggatcg aagattgaaa
      781 acggcgattc ccggacgatc aaagaggggg atgttctcac cgtgcgggcc ggcacgccgc
      841 atcagctgat tcttgcgccg ggcacgacgt acggggctgt tgtggtcaag atccacgagc
      901 cctgatcgga ccgaccgtaa gctcgggcta cggctcgggc ttacagtccg tagtgctgca
      961 ggaacggcga tggcgcgtgc gttctgcggc gttccctgac ccccagggag gcggaaattt
     1021 cccggcagca gcgcatcaga tgctccgcga taccgggcac actctgctgt gtgatccgcg
     1081 atgtgggacc cgaaacgctc acggctgcca tgggttgtcc cgcctcgttc aggatcggcg
     1141 ccccgatgca ccgcacgccc tcttccactt cctcgtcatc gatggcatag ccccggcgac
     1201 gaacccgttc cagtgagcgc atcaacgcct ccggcgtcat cagagtccgg tgggtatagc
     1261 gcacgaaccg aatcttcgcc atgatctccg tcgccatttc ctccggcagg aatgccatca
     1321 tggccttgcc catcgctgtg cagtagacgg gattcgacgt tccgatccgc gaagaaagcc
     1381 agacccgccg gttattcggc tcgactttat ccagatagac gactgaagtt ttttgcagaa
     1441 cccccagatg caccgtttcg cctacctgtg cggagagccg gcgaaaccag ggatggacgc
     1501 gcgcacgcaa atcgatctgc tccaccgcac gtgtgcccag ctcgtagagt ttcaggccaa
     1561 gccgaaaccg gttctccggc gtgcgctcga tcaacccgca ccgctccagc accatcaggg
     1621 cgcgatgcgc agtgcttttg tgcagatcca tccgctcgca cacgtccgcc aggcccagcg
     1681 ggccctcgct ctctccaagc agatccagca ccgacaccgc gcggtccagg ctctgcaact
     1741 gataaggatc ggagggcctt ttggctacct tctcttcgac acgctccggc tgtacttctg
     1801 ctttcttctg cctcatcgcc gacctcatcg tcatcgcgcc agccacccgc catccaccac
     1861 caggacggta cccgttacat aatcgctcgc cgccgaggag agaaacaccg ccgctccggc
     1921 aagatcttcc ggatcgcccc acctgcctgc gggaatccgc tcgaggattt gtcgattccg
     1981 cgtctcgtct ttctgcaggg cggaggtgtt ctcggtgcga aaatatccgg gcgcgatggc
     2041 gttcacctga acgttgtgtg gcgcccactc gttggccagc gctttggtca actgggcaat
     2101 cgcacctttc gatgccgtgt aggcagcgat gcgaatcccc ccctggaacg ccatcatcga
     2161 agcgatgttg atgatctttc cctgccgacg ctcgagcatt ttggccccga cgagctggca
     2221 gagccggaag acgctggtca ggttgacctg caggacctgt ttccaggcgt cgagatcgtg
     2281 atcggcggcc gccgcccggt gaatcatgcc ggcgttgttc accagaatgt ccggagcacc
     2341 catagccgcg gcgacttcgg agaacagctt gtccgctcct tcatcgctgc caagatccgc
     2401 ggagaagctt tgcccgtcgc ggccgatgtc gcgaattctc tgtgctgttt cttcagccgg
     2461 acggcgattc ccgtgacacg ccacagcagc gcctgcgtcg gcaaggccga gtgccatggc
     2521 cgcccctaat ccgctggccg ctcctgtgac cagagccgtt tttccatcca gcctgaaccg
     2581 ttcagcaatt cccatcgtcg atcggatcct cattccgcaa ccagggacgt tatccctcaa
     2641 tgcgaattcg caaagaagct attttctgga tgggggagag gggttgtcaa gcatctgcgt
     2701 gtgtgaagga agagaagcag gtccctccgc tccgccttcg ctcttgctca gggtccggtc
     2761 gggatgacag ggtgaaggag gagcagcagg tccctccgct tcggtcggga tgacagggga
     2821 cgggagttca ga
//
LOCUS       DASZYN010000629         2998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1059394, whole genome shotgun sequence.
ACCESSION   DASZYN010000629 DASZYN010000000
VERSION     DASZYN010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059394"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VHE33_16105"
     CDS             <1..319
                     /locus_tag="VHE33_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99029.1"
                     /translation="PAEALLAAVVQKGLEHDAGRIRYIQIGNSAGPNISLAAAALRSS
                     GLELYGSGFGSVSMDKLFASLRAILASAAKEAFQFKCTTAPLRDVEKLWNSKEEARLV
                     FVP"
     gene            458..841
                     /locus_tag="VHE33_16110"
     CDS             458..841
                     /locus_tag="VHE33_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99030.1"
                     /translation="MTSLNLIGGWLGMLAGVLSGALVGLFFYRDDWMGGYGSWRRRLT
                     RLGHISFFGLGFLNLIFAATAAQLLLAPPYLSTASWSFIVGAITMPLCCFLSAWRKPF
                     RHLFPIPVVSIAVGVVAILFGWWRK"
     gene            838..2184
                     /locus_tag="VHE33_16115"
     CDS             838..2184
                     /locus_tag="VHE33_16115"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HVT99031.1"
                     /translation="MKLGLIAMSGVRAHNPELTALGLTLPGFVERNKVIASLPSLGLL
                     TLAGLTPPSVDTHYLEVPDIAALDGVPAEFDVVAISSFSAQISEAYVLADRYRALGTT
                     VILGGLHVSALPDEARAHADAIVVGEGESAWPALVADLLHSGATLRPVYDARGCNFDL
                     ARSPMPRFDLLNIPNYNRLTVQTQRGCPFRCEFCAASIRLSPAYKVKPVDRVVAEIRE
                     IKRLWSRPFIEFADDNTFVNKPHSRRLMQALTHEDIRWFTETDVSVAEDPGLLSMMRD
                     AGCAEVLIGLESTSLSGVDGLEQRSNWKARQVDRYLDAIGSIQNHGIRVNGCFILGLD
                     GTGPDSFDAIAAFVQQSGLFDVQITVQTAFPGTPLYTRLRNEGRILRDPAWELCTLFD
                     VNFRPAKMSVSELECGFRALASQIYSEEATLARREHFRRNLHEQCRGEEKPGSAIY"
     gene            2213..2602
                     /locus_tag="VHE33_16120"
     CDS             2213..2602
                     /locus_tag="VHE33_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99032.1"
                     /translation="MTLLWIRIVLILCGAFDGVVGLIFFAAPAAMFRYFQVTPPNHYG
                     YVQFPALLLIIFAVIFFRAAADPVSRRDTILYGVALKASYSGLVFWYQAHGGVPSLWI
                     PWAWADAAFLVLLLLGWFALRTKPATA"
     gene            complement(2632..2937)
                     /locus_tag="VHE33_16125"
     CDS             complement(2632..2937)
                     /locus_tag="VHE33_16125"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HVT99033.1"
                     /translation="MDKQHFFFKLIPPRTTFPGDASAADLALMKQHADYFAGLFSAGK
                     VLLYGPVQSPAGAFGIAILEAENQAQAREIAANDPSVLGGLNRFEVAPMRVSAARAD"
BASE COUNT          496 a         1063 c          803 g          636 t
ORIGIN      
        1 gcccgcggag gctttgctcg ctgctgtggt ccagaagggc ctcgagcacg acgccggccg
       61 catccgttac atccagatcg gcaacagcgc cgggcccaac atctcgctcg cggctgcggc
      121 cctccgcagc tccggacttg aactctatgg cagcggattc ggcagcgttt ccatggacaa
      181 gctcttcgcc tcgctgcgcg ccatcctcgc ctccgcggcg aaggaggcat tccagttcaa
      241 atgcacgact gcgcctctgc gcgatgtcga gaagctttgg aactcgaagg aagaagcccg
      301 gctcgtcttc gtgccctgag cattttcagg agcgccacac gttgcccttg caatccagca
      361 ttcacgcagt agaatgcgag gcaaatccga tgcaggtgga tgggtttcac agcagcgctg
      421 cattatccgt ccaccgtagc cggagaaacg agcggtcgtg acatcgctga acctgattgg
      481 tggctggctg ggtatgttag ccggtgtgct ttccggcgcc ctggtcggtt tattcttcta
      541 ccgcgatgac tggatgggcg gctatggctc ctggcgccgc cgcctcacac gcctgggcca
      601 catttccttc ttcggactcg ggttcctcaa cctcatcttt gccgccacag ctgcccagct
      661 tctgctcgcg ccgccttatc tcagcaccgc atcctggtct ttcatcgttg gcgccatcac
      721 catgcccctt tgctgttttc tcagtgcgtg gcgcaaaccc ttccgtcacc ttttccccat
      781 cccggtggtc tccatcgccg tcggggtggt cgcaatcctc tttgggtggt ggcgcaaatg
      841 aaactcgggc tcattgcgat gagcggcgtt cgcgctcaca acccggagct caccgcgctg
      901 ggcctcacac ttcccggttt cgttgaacgc aacaaggtca tcgcatcatt gcccagtctg
      961 ggtctgctca cgcttgccgg cctcactccg ccttcggtcg acactcacta tcttgaggtc
     1021 cccgatatcg ccgcactcga cggcgttccc gcagaattcg acgtggtcgc tatctccagc
     1081 ttctcggctc aaatcagcga agcctatgtc ctcgctgacc gctaccgcgc gctcggaacc
     1141 acggtgatcc tcggcggcct gcacgttagt gccctgcccg acgaagctcg cgcccacgct
     1201 gacgccatcg tcgtcggcga aggcgaatcc gcgtggcctg ccctcgttgc tgatctgctc
     1261 cattccggcg ccactctgcg ccccgtatac gacgcacgtg ggtgcaactt cgatctcgct
     1321 cgttcgccca tgccgcgttt cgatctcctc aacattccca actacaaccg ccttacggtg
     1381 cagacccagc gcggctgtcc ctttcgctgc gaattctgcg ctgcttcgat ccggctttcg
     1441 cctgcctaca aagtcaaacc ggtcgaccgc gtcgtcgctg agatccgcga gatcaaacgc
     1501 ctctggagcc ggcctttcat cgaatttgcc gacgacaaca ccttcgtcaa caagcctcac
     1561 tcccgcaggc tcatgcaggc ccttacccac gaggacatcc gctggttcac cgaaaccgac
     1621 gtttccgttg ccgaggaccc cggcctgctc tcgatgatgc gcgacgccgg ctgcgccgag
     1681 gttctcattg gcttggagag cacctccctg tccggagtcg atggtctgga gcaacgctcc
     1741 aactggaagg cgcggcaggt cgaccgctac ctcgacgcca tcggctccat ccagaaccat
     1801 ggcatccgcg tcaacggctg ctttatcctt ggcctcgacg gcaccggccc cgactccttc
     1861 gatgccatcg ccgcatttgt ccagcaaagc ggtctcttcg acgtacagat caccgtacaa
     1921 accgccttcc ccggcacgcc tctctacacc cgcctcagga acgaaggccg catcctccgc
     1981 gacccggcct gggagctttg caccctcttc gacgtcaact tccgaccggc gaagatgtcc
     2041 gtctcggagt tggagtgcgg tttccgtgcg ctcgccagcc agatttactc cgaagaagcg
     2101 accctggcgc gtcgcgaaca tttccgcaga aaccttcatg agcaatgccg cggtgaggag
     2161 aaacccggca gtgccatcta ctgacgaaag ctaatcgtct gggagcagga ccatgactct
     2221 tctctggatt cggatcgtcc tcatcctctg cggcgcattc gatggcgtcg tcggcctcat
     2281 cttcttcgcc gcgcccgctg caatgttccg ttacttccag gtcacgcccc cgaatcacta
     2341 cggctacgtc cagttccccg cgctgctgct gatcatcttc gctgtgatct tcttccgcgc
     2401 cgcagccgac ccggtctctc gccgcgacac catcctctac ggagtcgccc tcaaggcctc
     2461 ctactcggga ctcgtgttct ggtatcaggc gcacggcggc gtgccatcgc tctggattcc
     2521 ctgggcctgg gcggatgccg cgtttcttgt gctgctgctc ctcggctggt tcgcactccg
     2581 gacgaaacca gcaacggcat agggggccac ggcacccggg cgagtcgcgc gctaatctgc
     2641 ccgcgccgcc gacactcgca tcggcgctac ctcaaaccgg ttcagcccgc ccaggaccga
     2701 aggatcgttc gcggcgatct ccctcgcctg cgcctggttc tcggcttcca gaatcgcgat
     2761 cccaaacgcc cccgccggcg actgcactgg cccgtagagc aacacttttc cggcagaaaa
     2821 caggccggca aaataatccg cgtgctgctt catcagcgcc aggtccgcgg cgctcgcatc
     2881 gcccggaaag gtcgttcgcg gcggaatcag tttgaagaag aagtgctgct tatccatggt
     2941 gcagccatct taagctgctt ccagcttcca cctgctcctg actacgggcc attggttc
//
LOCUS       DASZYN010000630         4224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_549214, whole genome shotgun sequence.
ACCESSION   DASZYN010000630 DASZYN010000000
VERSION     DASZYN010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549214"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VHE33_16130"
     CDS             complement(<1..372)
                     /locus_tag="VHE33_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99034.1"
                     /translation="MAEFDHGKVRKESLTAISPTLTLTSMRSLFTAALGIFLSWSVFA
                     AEQPAGTGPSFKGPLGLQMYSLRFYSPSNFLGKLDKVQEYGLRTIEGGPPARGMSREE
                     FFTELDKRGIKLVSSGVDYARL"
     gene            2073..4199
                     /locus_tag="VHE33_16135"
     CDS             2073..4199
                     /locus_tag="VHE33_16135"
                     /inference="COORDINATES: protein motif:HMM:NF044877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVT99035.1"
                     /translation="MAEAFAMVSLIEQGWADQKEVAQAFDCSARTVRRHQRRYESGGL
                     AALGHESGYPQGRCRSRGGRTQLIQRLKAEGHSNREIARRLGVSETAIRKMLRRLGWL
                     ELTPTSDLLPLDVGSSVAPHHSPALPSPEQSVPVSASAENPTAPAVRATFDTDPMDRS
                     GDRLLAYLGLLEDAAPLFGSRANVARAGVLLAVAPLVQSGVFMCAQQIYGSVGPAFYG
                     LRTSLLTLLLMALWRIRRPEALKEHSPQELGRVLGLDRAPEVKTLRRKLSRLAAAGRA
                     TQFGQALAQSRIARRGEALGFLYVDGHVRVYHGQHRLPKAHVARMRLSMPATTDYWVN
                     DTRGDPLFVVTAEANAGLVKMLPGVLEQLRQFVGKRRVTVVFDRGGFSPRLFQQIMAA
                     GFDLLTYRKGHCRRVPRKRFQTCQTRVEAKTSTYQLADQEVRLLKGKLRLRQVTRLAE
                     DGHQTPILTSRRDLPPAQIAYRMFARWRQENFFKYLREEYALDAVADYSAVPDTSNRE
                     IPNPAWAAADAKLRQAWAYLERLNAEYGLEAVNNLERLRRTMRGFKVAHGKLGQSIAK
                     AMEQVLACQKKRDALPRRIPLQQLPGEPVMKLPPERKHLTNLLKMVAYQAESDLVRIV
                     APHYRRVDDEGRTLIQSALSSAADLEVTATELRVTLAPLSSPHRTRAIAALCRQLNQS
                     ATLFPGSQLRLRYAVREHSQQKSGHF"
BASE COUNT          866 a         1247 c         1200 g          911 t
ORIGIN      
        1 tagtcgcgca taatccactc ccgagctgac cagtttgatc ccgcgtttat cgagctccgt
       61 gaagaactcc tcccgcgaca tcccgcgggc tggcgggccg ccttcaatag ttctaaggcc
      121 gtattcctgg accttatcga gcttccccag gaagttgctg ggtgagtaaa atcgcaggga
      181 atacatctgc aagcccagcg gacccttaaa actcggacca gtcccggccg gctgttctgc
      241 cgcaaagaca ctccaggaca gaaaaatgcc gagcgcggcg gtaaatagag atctcatgga
      301 ggtgagcgta agggtggggg aaattgcggt caatgactcc ttccgaacct tgccgtgatc
      361 gaattcagcc atgccatccg cttgaggaga aagggtatcc cgccgttagc acggaggtta
      421 taacaccctt cgaaacgacc ctccacgaca gaggcaaggc tctctccatc aaacagatgt
      481 cgtgatgctg accaaattta aaatcgtgcg atcttcccgc gatcaggtct tctttcgacc
      541 ggggaatccg ggccgcgcga aactggagga gcggtgtggg ctgacgtgga cgtgtaaaaa
      601 tgcgcctgcc gctgtgatga gatgcagctc ggaagattcg gccgcgccgg tctcgatctc
      661 gactttacag gtagttcaca ggcacttcac aggcacttca caggcgacgg gagcgagtat
      721 accactcgtg aagtgcctgt gtactacccg gcaagtcggt atccagtccc gccctactgc
      781 gcctccacga aatcacaagt cgaatcgcgg gcgtccccgt ccgcagcatc gcccgccaga
      841 atgcccgaat tacaaatctc cgatgctgtg cgggcgcggc aagctggtct ggctgattac
      901 ggcacaccgt cgcgggcgga agacttgcgg ccacccgtta gcttcaccag attgggcatt
      961 agcacggatg ttataacacg agagaggtaa cccagctcgc aaacgtgcaa tcgattgccg
     1021 cagaggcgat tgtggaatgg tgaccaaaat caatttcggc cgccatgatg gacagagcgc
     1081 gcaacaggct tggagacggc tctcgccaaa cactcgcctc acaccgcacc aacgacggag
     1141 atcaagccgc tttgggcgct ccatccgtcc aaccttgccg tgaatggacc cgtcaccttc
     1201 cagagcgcgg cgcgagcatc cgccgtcaga gccggcctgc cagaaagcgg gccaccgcct
     1261 cggcccgaga gcggacatgg agtttctcgt atacgtttcg aacgtgtgta ttgacggtgg
     1321 tgacactaat gcccaactga tccgctgctt ctttggatcg atatcctcga gctaagagcc
     1381 cgaggatctc tgattccaga tctcctgaca tagaaggatc gggatctagg atttcctgag
     1441 catttttcgg attccggggt gcgtgaggtg cgaacctaaa cctgtccgct ttttccgccg
     1501 ctgtcccaaa gccggtgctg acttccaggg gtttcctgag catttgcaga ttccggggtg
     1561 cgtgaggtgc gaacctaaac ctgtccgctt tttccgccgc tgtcccaaag ccggtgctga
     1621 cttccagggc tttcctgagc atttttcaga ttccgaggtg cgtgaggtgc gaacctaaac
     1681 ctgtccgctt ttccgtcgct gtcccaaaag ccggtgctga tttccagggg tttcctgagc
     1741 attttgcagg ttccggggtg cgtgaggtgc gaacctaaac ctgtccgctt tttccgccgc
     1801 tgtcccaaag ccggtgctga tctccagaga tttcttgagc attttgcgga ttccgaggtg
     1861 cgggaggtgc gaacccaaac ctgtccgctt tttcaggagc cggcctatgc tcaggcatgt
     1921 ccagtggtcg cagcgatgac ttgtttccca atgtgcgcaa cccgcaggct ctccgagtca
     1981 taaatgagcg gtgtctcgtt cgaactcagg atggctatcg ggtggtctgc gtctctggcg
     2041 ttgtcttggc acaatatgcc gtgagcgatc acatggccga agcgtttgct atggtcagtt
     2101 tgatagagca aggttgggcc gatcagaagg aggtggcaca agctttcgat tgttccgccc
     2161 gcaccgtgcg ccgacatcaa cggcgttatg agagcggcgg cctggctgcc ttgggtcatg
     2221 agagtgggta tccgcaaggt cgctgtcgct cacgaggagg acgaacccaa ctgattcaac
     2281 gcctcaaagc cgaaggccac tccaaccgtg aaattgctcg ccgcctcgga gtcagtgaga
     2341 cggccatccg caaaatgctg cgccggctgg gttggttgga actgactcca acatcggatt
     2401 tgttgccttt ggacgtgggg tcgagcgtcg ctccgcacca ctctcctgct ttaccttcgc
     2461 cggagcaatc tgtgccggtg tcggcttctg ctgaaaatcc aacggccccc gctgtgcgcg
     2521 ctactttcga taccgatccg atggatcgat ccggggatcg cctgctggcc tatttgggat
     2581 tgctggagga tgccgcacca ctgttcggtt ctcgggccaa cgtggctcgc gccggtgttc
     2641 tattggccgt tgcaccattg gtgcagagcg gcgtctttat gtgtgcccag caaatctacg
     2701 gcagcgtcgg tcctgccttc tacggtctgc gcaccagttt gcttaccctg ctgttaatgg
     2761 cgctctggcg catcagacgg cccgaagcac ttaaagaaca ttctccccaa gaactgggac
     2821 gagtcttggg attggatcgt gcgcccgaag tcaaaacact ccggcgcaaa ctatctcgtt
     2881 tggctgccgc tggacgggcc acgcagttcg gacaagcttt ggctcagagc cggatcgctc
     2941 ggcgcggaga agctctgggc tttctttacg tcgatggtca cgtgcgcgtc taccatggcc
     3001 agcaccgtct gcccaaggca cacgtggccc ggatgcgtct gtccatgccc gcgaccaccg
     3061 actactgggt taacgacacc aggggcgacc cgctttttgt cgtgaccgcc gaggccaacg
     3121 ccggattggt gaaaatgctg cctggagttc tggagcagtt gcgccaattc gtgggcaaac
     3181 gtcgtgtcac cgtcgtcttt gatcgcggcg gctttagtcc gaggctcttt cagcagatca
     3241 tggccgctgg ttttgatctg ctcacctatc gtaaggggca ctgccgccgg gttccgcgca
     3301 agcgctttca aacgtgccag acgcgcgtgg aagcaaagac cagcacctat cagctggctg
     3361 accaggaagt gcgcttgctc aagggcaagc tccgcctgcg ccaagtcact cgtctggccg
     3421 aagatggaca ccaaacgccc attctgacct cacggcggga tttgccacca gctcagatcg
     3481 cctatcgtat gttcgcacgc tggagacagg aaaatttttt taagtattta cgcgaggaat
     3541 acgctttgga tgcagtcgcc gactattccg ctgtgccgga tacttccaac cgagagatcc
     3601 ccaatcccgc ttgggcggcc gccgatgcca aactccggca agcctgggcc tatttggaac
     3661 gtctgaacgc cgagtatggc ttggaagctg tcaataacct cgagcgcctc cgtcgaacca
     3721 tgcgcggatt taaggtcgcc cacggcaagt tgggacaaag catcgcaaaa gccatggagc
     3781 aagtgctggc ttgccaaaag aagcgagacg cactgcccag acggattcca ctccagcagt
     3841 tgccaggcga gccggtcatg aagctgcctc cggaacgtaa acatctgact aacctcctta
     3901 aaatggttgc ctatcaggcc gagagtgatc tcgttcgaat agtcgctcct cattaccgga
     3961 gagttgatga tgaaggacga accctcattc aatcggccct gtccagtgcg gcagacctgg
     4021 aggtaaccgc gaccgaattg cgcgtcaccc tggccccctt gagttcacct catcgaacac
     4081 gcgcgatcgc ggcactctgc cgacaactca accagtccgc cacgctgttt ccaggatccc
     4141 aactgcgcct gcgctacgcc gtccgggagc attcgcagca aaaaagcgga catttttgaa
     4201 cggctctatg tcaggagttc tgga
//
LOCUS       DASZYN010000631         3253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_227942, whole genome shotgun sequence.
ACCESSION   DASZYN010000631 DASZYN010000000
VERSION     DASZYN010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227942"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1680)
                     /locus_tag="VHE33_16140"
     CDS             complement(<1..1680)
                     /locus_tag="VHE33_16140"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HVT99036.1"
                     /translation="MGLLSGRNDPHRLGPMQTLPGPMKTWPENEPVDYCIVGVGSAGG
                     VLVQRLARAGFRVVGLEAGPFWDTEKDWVSDEKGSHELYWNDLRITGGTDPLALGENN
                     SGKGVGGGSVHWAAFTPRFHPSDFRVYSEDGVGVDWPMSYWDLKPWYELLEREIPVAG
                     PAWFPWGDPHGHPYGPHPLGGVGDVLVRGCTKLGIRTVAGGPVAILSASHGDRPHCIY
                     RGFCLQGCKVGAKASTLITHVPDAISHGAEIRAHSMAARINVRSDGRVDGVVYLDRDG
                     REHFQRARAVIVSGYAIETPRLLFNSACPGHSHGLANSSGTLGKYLMAQAGNVVAGRF
                     DELIRLYKAPPAHALSEEFYETDAKRGFARGFAIQTVGPLPIAFAKQMVAAMGAWGWG
                     LRRVMMDYNHWATLGVLGEILPWEDNRVEVADEKDQYGLPVAKVTFNLHENDKKLIEF
                     GKNKVMEVMWAAGAKQVVQESRFAHLVGAVRMGNDPSASVVDKFGRTHDIPNLFVCDG
                     SILPTQGSANPGLTIQALAARTADYLIGQGDAIFFSNRRDAQEPRIRYELSP"
     gene            complement(1680..2432)
                     /locus_tag="VHE33_16145"
     CDS             complement(1680..2432)
                     /locus_tag="VHE33_16145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HVT99037.1"
                     /translation="MSKRSFPTRATGGEIRDLPQPGYYSAYSTLGQKKAWDKTTRNVV
                     TSRVGEVPAIRFFSREESEILQAVIDRVLPQDDRCEERTIPILPVIDERLFKNALNGF
                     RYEDMPPDRDAYRLGIEAIDDMARERFHARFPSLTVHRQELILKSLHDGAPDPAHPAW
                     KRMPVHRFWALLMHDAITAYYSHPWAWDEIGFGGPAYPRGYMRLENGLPEPWETGEER
                     YEWNVPVDSLSELDPEGAPPEHGSLHGHGGTH"
     gene            complement(2429..>3253)
                     /locus_tag="VHE33_16150"
     CDS             complement(2429..>3253)
                     /locus_tag="VHE33_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99038.1"
                     /translation="VASGLEVGYEHYKGSYSNPVMYTPVALSGALAAAGLSGVFSRRA
                     ARTLLRWTSAVTLADGIIGFFFHVRGVARKPGGWRLPVTNIVMGPPIFAPLLFGTSAY
                     LGLMASYLRREEDLGGVAERVRDAAEAVLPVRPDWREDLRNGRFQKHLCGVTIIWAFC
                     SGFEALYSHYKSNFRVWAQWTPVLLTPPLIAAAAGAIRSRRIANTVLPIISALALADG
                     AAGFYYHARGILGRPGGTKKPLYNVLYGPPIFAPLLFAACGFLGLMASLLRREREK"
BASE COUNT          627 a         1023 c         1009 g          594 t
ORIGIN      
        1 cgggctgagc tcgtagcgaa tcctgggttc ctgcgcgtca cggcgattcg agaaaaagat
       61 cgcatcgccc tgaccgatca ggtagtcggc ggtccgcgcg gccagcgcct ggatcgtcag
      121 ccccggattg gcagaaccct gcgtcggcag gatacttccg tcgcagacga agaggttggg
      181 gatgtcgtgg gttcgtccga acttgtcgac gaccgaggca gatgggtcgt tgcccatgcg
      241 cactgcgccg accaggtgcg cgaagcgcga ctcctgcaca acctgcttcg cgcccgccgc
      301 ccacatcact tccattactt tgttcttgcc aaactcgatg agcttcttgt cattctcgtg
      361 aagattgaac gtcaccttcg cgacgggcaa cccatactga tccttttcgt ccgccacttc
      421 cacgcgatta tcttcccagg gaagaatctc accgagcacc ccgagtgtcg cccagtggtt
      481 gtaatccatc atgacgcggc gcaggcccca gccccacgcc cccatcgcgg ccaccatttg
      541 tttcgcgaac gcgatcggta acggaccaac ggtctggatc gcaaatccgc gcgcgaagcc
      601 gcgtttcgca tccgtctcat agaattcctc cgacagggca tgcgcaggcg gcgctttgta
      661 gaggcgaatc agctcgtcga atcgccctgc gaccacgttg ccggcctggg ccatcaggta
      721 cttgcccagg gttccgctgg aattggccag cccatgactg tgacccgggc atgcggagtt
      781 gaagagcaga cgcggcgttt caatggcgta gccgctgacg atcacagccc gcgcgcgctg
      841 gaagtgttcc ctgccatcgc gatccagata gacgactcca tcgacgcggc cgtcgctgcg
      901 cacattgatc ctcgcggcca tcgaatgcgc tcgaatctcg gcgccgtggc tgatggcgtc
      961 tggaacatgc gtgatcagcg tgctcgcttt ggcgccgacc ttgcacccct gcaggcaaaa
     1021 tccgcggtag atgcagtgcg gacggtcgcc atgcgaggcg gacaggatgg cgacgggacc
     1081 accggcgacg gtgcgaattc caagtttggt gcagccacgc accagaacat cgccgacacc
     1141 gccgagcgga tgggggccgt agggatgtcc gtgcggatcg ccccagggaa accatgcggg
     1201 accggcgacg ggaatctcgc gctccagcag ttcataccag ggcttgagat cccagtacga
     1261 catcggccag tcgacgccga cgccgtcttc gctgtagacg cggaagtcgg acggatgaaa
     1321 acgcggagta aacgcggccc agtgcactga gccgcctccc acgcctttgc cgctgttgtt
     1381 ttcgccaagc gcgagcggat cggtgccgcc ggtgatgcgc agatcgttcc agtaaagctc
     1441 gtgcgagcct ttttcatcgc tgacccagtc tttctcggta tcccagaagg gcccggcttc
     1501 gaggccgacg acgcgaaacc cggcgcgagc caggcgttgg acgagcacgc ctccagcgga
     1561 gccgacgcca acgatacagt agtcgaccgg ctcattctcc ggccaggttt tcatcgggcc
     1621 tggcagggtc tgcatggggc cgaggcgatg cgggtcgttt cggccggaga gcaatcccat
     1681 cagtgtgtgc ccccgtgtcc gtgcaatgac ccgtgctcgg gcggcgcgcc ctcgggatcg
     1741 agctcggaga gcgaatcgac gggaacattc cattcgtagc gctcttcgcc ggtttcccac
     1801 ggttcgggca atccgttttc gagtcgcatg tagccgcggg gataggcggg tcctccgaaa
     1861 ccaatttcat cccaggccca ggggtgcgaa taatatgccg tgatggcgtc gtgcatcagc
     1921 agggcccaga agcggtggac cggcatccgc ttccacgccg gatgagccgg atcgggtgcg
     1981 ccgtcgtgca gcgatttcag gatgagttcc tggcggtgaa cggtaaggga agggaaccgc
     2041 gcatggaaac gttcgcgcgc catatcgtcg atcgcttcaa tgcccaaccg ataagcatcg
     2101 cgatccggag gcatgtcctc ataccggaag ccgttcagcg catttttgaa gaggcgctcg
     2161 tcaatgacag gaagaatcgg gatggtgcgc tcttcgcatc gatcatcctg cggcagcacc
     2221 cgatcgatga cagcctgcag aatttcggat tcttcgcgcg agaagaaccg gatcgccgga
     2281 acctcgccga cgcggctggt aacaacattc cgcgtggtct tgtcccaggc tttcttctgt
     2341 cccagcgtgc tgtaggcgga atagtagccg ggttgcggca gatcgcgaat ttcaccgccg
     2401 gtggcccggg tggggaacga tcgtttgctc atttttctcg ctccctgcgc aacaagctcg
     2461 ccatcagtcc cagaaagccg cacgctgcaa acaaaagcgg tgcgaaaatc ggcgggccgt
     2521 agagaacgtt gtacagcggt ttcttcgtgc cgccaggacg gccgagaatg ccgcgcgcgt
     2581 ggtagtagaa gccggccgcg ccgtcggcta aagccagggc ggagatgatt ggcagaactg
     2641 tgtttgcaat ccgccggctc cggatcgcgc cggccgcagc agcgatgaga ggcggcgtca
     2701 gcaacaccgg cgtccattgc gcccaaacgc gaaaattcga tttgtaatgc gaatacagcg
     2761 cttcaaaccc gctgcagaat gcccagataa tcgtcacacc gcagaggtgt ttctggaaac
     2821 gaccgttgcg aagatcctcg cgccagtccg gccgcaccgg aagcacagct tcagcggcat
     2881 cgcgaacccg ctcagcgacg cctccgagat cttcttcgcg gcgcaggtac gaggccatca
     2941 ggcccagata ggcgctggtg ccgaagagca gcggcgcgaa aattggtgga cccatcacga
     3001 tgttcgtcac ggggagtctc cagccgcccg gcttgcgcgc cacgccgcgc acatggaaga
     3061 agaatccgat gattccgtcc gcgagggtga ccgcactggt ccagcgcagc aaagtccgcg
     3121 cagcccggcg gctgaatacg cctgaaagtc ctgcagcggc cagcgctccg ctcagcgcga
     3181 ccggtgtgta cattaccgga ttgctgtagc tgcccttgta atgttcgtaa ccgacttcga
     3241 ggccgctggc cac
//
LOCUS       DASZYN010000632         6216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_734974, whole genome shotgun sequence.
ACCESSION   DASZYN010000632 DASZYN010000000
VERSION     DASZYN010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734974"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1401)
                     /locus_tag="VHE33_16155"
     CDS             complement(187..1401)
                     /locus_tag="VHE33_16155"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HVT99039.1"
                     /translation="MTKLSIRDLDLAHKHVFMRVDFNVPLTEDGAEITDDTRIKETLP
                     TIEYALRHKAKLILASHLGRPKGKVNPKYSLRPVVDRLRTLLDHRLGEKTNVAFAPDC
                     VGDIAKELARQLESGQVLLLENLRFHPEEEANDPEFSKKLASLCELYVNDAFGSAHRA
                     HASTEGITHFVKQSAAGLLMEKELTYLGKALEAPARPFVAIIGGSKISGKIDVINNLL
                     DKVDTLVIVGGMAYTFQRALGVTTGKSLVEEDRIEMAGEALKKAKAKGVELLLPVDNV
                     LADSFSNDAKTQPWDTSKDFPADWQGLDIGPKSVAAIQKIVAGARTIVWNGPAGVFEF
                     PKFAVGTNAIAKAVAENQDAISIVGGGDSVAAVKQAGVADQIKHISTGGGASLEFLEG
                     KKLPGVEALTGK"
     gene            complement(1507..1920)
                     /locus_tag="VHE33_16160"
     CDS             complement(1507..1920)
                     /locus_tag="VHE33_16160"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HVT99040.1"
                     /translation="MGALRDWEGSMRQNIPGTSPYEPVIGFSRAVRAGQTVYVSGTGP
                     VGADLADAATQTRHILEIIRGTLAKAGARLEDVVRTRMFITHLEDWEAIGKAHGEFFG
                     TIRPAATMVIVAGLLQPTWRVEIEAEAVVHSAPHE"
     gene            complement(1951..2955)
                     /gene="gap"
                     /locus_tag="VHE33_16165"
     CDS             complement(1951..2955)
                     /gene="gap"
                     /locus_tag="VHE33_16165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HVT99041.1"
                     /translation="MAVKVGINGFGRIGRNVLRASLGNPDIDFVAVNDLTSPATLAHL
                     LKYDSILGNLKNEIVAGEDFISIDGKKIKVFKEKDPALLPWDSVGAQIVVESTGHFTD
                     ATKAKAHLRGPVKKVIISAPATNEDLTVVLGVNEKKYDPAKHNVISNASCTTNCLAPV
                     VKVLVENFGIVSGIMTTIHSYTNDQVILDFPHKDLRRARAAALSMIPTSTGAAKALKL
                     VIPEVEGKLDGFAIRVPTPNVSVVDLTFVAEKPITKDAINAALKAASEGELKGILGYD
                     TNELVSSDFKGNPLSSIVDAPLTKVVGNTAKVISWYDNEWGYSCRVRDLILFLTKKGL
                     "
     gene            3249..4043
                     /locus_tag="VHE33_16170"
     CDS             3249..4043
                     /locus_tag="VHE33_16170"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVT99042.1"
                     /translation="MRRRSKRGPNNSESTGKIGQELPPNQPAPLQPSYHGGAAAPAED
                     EIEVAPEAEPAEPVAEVPPEPVAALAASSPEPAGKMVLETQPESLSTPPPEPVAGNKG
                     SRGFVVLTIGLPGSGKTTWFKRRGVTPLSSDLLRTLLFDDIAEQRWQGLVFSTLRSLL
                     RARLIAKMPWNYVDATNLSPHERRQWIKMAKSFGYEVQAVFFDVPLEVCMERNKRRER
                     TVSDDAMKKLADRLRPPDFKEGFSKITVVRVKGAGPGGAATSSEPE"
     gene            complement(4065..4457)
                     /locus_tag="VHE33_16175"
     CDS             complement(4065..4457)
                     /locus_tag="VHE33_16175"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT99043.1"
                     /translation="MVKIVLTSMLVDDQEKALRFYTEKLGFVKKEDIPMGEHRWLTVV
                     APGDRDGVELVLEPDAHPAAGPFKKALVEDGIPYTSFGVDDVQAEYERLRAAGVVFTQ
                     PPVAMGPVTMAVLDDTCGNLIALAQRHA"
     gene            4456..4824
                     /locus_tag="VHE33_16180"
     CDS             4456..4824
                     /locus_tag="VHE33_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99044.1"
                     /translation="MVLSPWFLLQPAVDDRPQRVPPEVVELVAALARRRDQACLFEHF
                     QMLRDGLARRCDAMLHRQARAQLEQRLPVALRQLVQNRPPCRRCHCLKNVDHSITIGK
                     SQLACQDVCLATLWLTEGVH"
     gene            complement(4805..5374)
                     /locus_tag="VHE33_16185"
     CDS             complement(4805..5374)
                     /locus_tag="VHE33_16185"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HVT99045.1"
                     /translation="MKVPYAKKWQKETDQLRKIALDCFLTEERKWSKPCFTFQKKNVA
                     IIIPLRDSCALSFFKGALLRDPKKILQRVGEHTQAGRWIKFTSVREITALQSTLRAYL
                     YEAIELEESGRKVVLKKPADYPVPEELQALLEASTRLRTAFEALTPGRRKSYIFHIAG
                     AKQTKTRAARAARCVPMILGGRGFNERPR"
     gene            complement(5947..>6216)
                     /locus_tag="VHE33_16190"
     CDS             complement(5947..>6216)
                     /locus_tag="VHE33_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99046.1"
                     /translation="DRGDASPGHISDLTAGRLIRALLNRLGDVPFPDPELAPPSTPVP
                     SPRAASANPLPASPSEMRELKAMLAALGMPASQSPMHQSPARVNQ"
BASE COUNT         1119 a         2044 c         1768 g         1285 t
ORIGIN      
        1 accttcttcc tcaccaccca ctggcgcatc tacggcacta tcatcgcctt ggtcggctcg
       61 atcctggggg cgctgtatcc ggcctggaaa gccgcgagca aagaccccat cgacgccctc
      121 gcctacgaat agcaaaacaa aggcgcaggt ccggttggac ctgcgccagg ccgtgccgaa
      181 aataatctac ttcccggtca gcgcctccac ccccggcagt ttcttccctt ccaggaactc
      241 caggctcgcg cctccgccgg tcgagatgtg cttgatctga tccgcgactc cggcctgctt
      301 cactgccgcg accgaatcgc caccaccgac gatcgagatg gcatcctggt tctcggcgac
      361 tgctttggcg atcgcgttcg tgcccacggc gaacttcgga aactcaaaga ccccggccgg
      421 tccgttccac acgatcgtgc gcgccccggc gacaatcttc tgaatcgccg ccaccgactt
      481 tggcccgata tccagcccct gccagtccgc cggaaagtcc ttactcgtat cccacggctg
      541 cgtcttcgcg tcgttcgaga agctgtcggc cagcacgttg tccaccggca gcagcagttc
      601 cacgcccttc gcctttgcct tcttcagagc ctcacccgcc atctcaatgc ggtcttcttc
      661 caccagcgac ttgccggtcg tgacgcccag cgctctctgg aacgtgtacg ccatgccgcc
      721 gacgatcacc aatgtgtcga ctttgtccag caggttattg atgacgtcga tcttgcccga
      781 aatctttgac ccgccgatga tggccacgaa aggacgcgcc ggcgcctcca gcgccttgcc
      841 caaataggtt agttccttct ccatcagcag gccggccgca ctctgtttga cgaagtgcgt
      901 gattccctcc gtcgaagcat gcgcgcggtg agccgaaccg aaagcgtcgt tcacatagag
      961 ctcacacagc gatgccagtt tcttcgagaa ctcgggatcg ttggcctctt cctcgggatg
     1021 gaagcgcagg ttttcaagca gcaacacctg tccggactcg agctgccgcg cgagctcttt
     1081 cgcgatgtca ccgacgcagt ccggcgcaaa cgccacattc gtcttctcac cgaggcgatg
     1141 atccagcagc gtccgcagcc gatccacgac cggacgcagg ctgtactttg gattcacctt
     1201 gcccttgggc cgccccaggt gcgacgccag aatcagcttc gccttgtgcc tgagcgcgta
     1261 ctcaatggtg ggcagcgtct ctttgatgcg cgtgtcgtcg gtgatctccg cgccgtcctc
     1321 ggtcaatggc acattgaagt ccacccgcat gaacacatgc ttgtgcgcca gatccagatc
     1381 gcgaatcgat agtttcgtca taacgccctc agtctccact gcactcgcca ttgtgccgat
     1441 cctccttctt cgccggttac agaaactctc tccgccgcta agacggtccc ttcgtgctct
     1501 gtgcccttac tcgtgcgggg ccgagtgcac cacggcctcc gcctcaatct cgacgcgcca
     1561 cgtcggctgc agcaatccgg ccacaatcac catggtggcc gccggccgga tcgtcccaaa
     1621 aaactcgccg tgggccttgc cgattgcttc ccaatcttca agatgcgtga tgaacatacg
     1681 cgtccgcact acatcctcca gccgcgcccc cgccttggcc agcgttccgc gaatgatctc
     1741 cagaatgtga cgtgtctggg tcgccgcgtc ggccagatcg gcgccgaccg gcccggtgcc
     1801 tgaaacatac accgtctgcc ccgcacgcac cgcgcgcgag aatccaatca ccggttcata
     1861 cggagatgtg cccggaatat tctgccgcat ggagccctcc cagtcgcgca gggcccccat
     1921 cgggagcccc acacaagagc agcagttgaa ctacagaccc ttcttcgtga ggaacaggat
     1981 caggtcgcgt acccggcacg agtagcccca ctcattgtcg taccagctga tgaccttcgc
     2041 cgtgttgccc acaaccttgg tcagcggtgc atccacgatc gacgacagcg gattgccctt
     2101 gaagtcgctc gacaccagct cgttcgtgtc gtatcccagg atccccttca gttcgccctc
     2161 tgaggctgcc ttcagcgcgg cgttaatcgc gtccttcgtg atgggcttct cggccacaaa
     2221 ggtcagatcg accacggaga cgttcggcgt ggggacgcgg atcgcgaacc catccagctt
     2281 gccttcgact tcagggatga ccagcttcag cgccttcgcc gcgcccgtcg aggtcggaat
     2341 catcgacaga gccgccgcgc gtgcgcgccg caggtcttta tgcgggaagt ccaaaatgac
     2401 ctggtcgttg gtgtagctgt ggatggtcgt catgatgccc gacacgatgc cgaaattctc
     2461 caccagcacc ttgaccaccg gcgccaggca gttggtcgtg cacgaggcgt tcgaaatgac
     2521 gttgtgcttc gccggatcgt acttcttctc gttgacgccg agcaccacgg tgaggtcctc
     2581 gttggtcgcg ggagcggaga tgatcacctt tttcaccgga ccacgcagat gcgctttggc
     2641 cttggtggcg tcggtgaagt gcccggtcga ctcgaccacg atctgcgcgc cgaccgaatc
     2701 ccacggaagc agcgccggat ccttttcttt gaagaccttg atcttcttgc cgtcgatcga
     2761 gatgaaatcc tcgcctgcga cgatctcgtt cttcaggttg cccagaatcg agtcgtactt
     2821 cagcagatgc gccagggttg cgggactggt cagatcgttc actgccacaa aatcgatgtc
     2881 cggattcccc agcgacgccc gcagcacgtt ccgcccaatg cgtccgaacc cgttgatgcc
     2941 aaccttcacg gccatagaaa ttcgtctcct tgcctcaatt tttcagcctt gcggtcacaa
     3001 ctcgcggtga tccggatcac gttgatttcg aacggtcgaa aacgcccgga accacccatt
     3061 taccgatgct agcaccgtag tcaatgaaaa agtgcagcag gcaccgcgtt cggccacatt
     3121 tccgcattcc tgcgtcacat ctttttcaca aaaaccgtgc ctcttgtggc acatagcacc
     3181 actgtgcgtt tgctccgcgt cgatgtctgt gttacatcct ggatatgcgc ctgtggatgc
     3241 gcgaccgaat gagacgtcgc tcgaagcgtg ggccgaacaa ttcagagtcc actggaaaaa
     3301 ttgggcagga attgcccccg aatcagccag ctcccctcca gccttcctac catggtggcg
     3361 ccgccgcgcc tgcggaagac gaaatcgaag tcgcgccgga ggccgaaccc gctgaacctg
     3421 ttgccgaagt accgcctgaa ccggtggccg ccctcgccgc gtcctctcct gagccggcgg
     3481 gcaagatggt gctcgaaacg cagccggaat ccctttcgac accgccgcct gaaccggtgg
     3541 cgggcaataa aggctcccgt ggatttgtcg tcctcacgat tggcctgccc ggatccggaa
     3601 aaacgacctg gttcaagcgc cgtggcgtca cgccgctctc gagcgacctg ctccgcacgc
     3661 tgctcttcga cgacatcgcc gagcagcgct ggcagggcct cgtcttctcg actctgcgct
     3721 cgctgctgcg cgcccgcctg atcgcgaaaa tgccctggaa ctatgtcgac gcgacgaacc
     3781 tctcgcccca cgagcgccgc cagtggatca agatggcgaa gagtttcggc tacgaggtgc
     3841 aggccgtgtt cttcgatgtc ccgctggaag tttgcatgga gcgcaacaag cgacgcgaac
     3901 gcacggtcag cgacgatgcc atgaagaagc ttgccgaccg gctgcgtccg cccgatttca
     3961 aggaaggctt ctcgaagatc accgtggtgc gcgtgaaggg cgccggaccg ggcggagccg
     4021 cgacgagcag cgaaccggaa taagcctggc gggcacgcgg ccgttcatgc gtgccgctgc
     4081 gccagcgcga tcagattgcc acaggtatcg tccagcaccg ccatcgtcac cggacccatg
     4141 gccaccggcg gctgcgtaaa caccactccc gcggcccgca gcctctcgta ctccgcctgc
     4201 acgtcgtcca ctccaaacga cgtgtacgga attccatcct ccaccagggc ctttttgaat
     4261 ggcccggccg ccggatgcgc atccggctcc agcaccagct ccactccgtc acgatcgccg
     4321 ggcgcgacga cggtcagcca gcggtgctcg cccatcggga tgtcttcctt ttttacgaac
     4381 cccagcttct cggtgtagaa gcgcagcgct ttctcctgat cgtcgaccag catgcttgtg
     4441 agcacgatct tcaccatggt tctctctccc tggtttcttt tgcagccagc ggtcgacgat
     4501 cgtccacagc gcgtccctcc ggaagtagtg gaacttgtag cggccctggc gcgacgtcgc
     4561 gaccaggcct gcctcttcga gcacttccag atgctgcgag atggcctggc gcgtcgctgt
     4621 gatgccatgc ttcatcgcca ggcgcgcgca cagctcgaac agcgtctgcc cgtcgcgctc
     4681 cgccagctcg tccagaaccg cccgccttgc cggcgatgcc actgcctcaa aaacgtcgac
     4741 cactccatca caataggcaa gtcacagctt gcctgtcaag atgtgtgtct ggcgacgctc
     4801 tggcttaccg agggcgttca ttgaagcccc gtccaccgag gatcatgggg acacatcggg
     4861 ctgcccgcgc ggcccgcgtc tttgtctgtt ttgcgcctgc gatgtggaag atgtacgact
     4921 tgcggcggcc cggcgtgagc gcctcgaatg ccgtcctgag gcgcgtgctg gcttcgagca
     4981 aagcctgcag ctcctccggg acggggtagt cggccggctt cttcagtacg acctttcggc
     5041 ccgactcttc cagttcgatc gcctcataaa ggtaggctct cagcgtcgac tggagagctg
     5101 tgatctcccg gaccgatgtg aacttgatcc agcgtccggc ctgcgtgtgt tcgcccaccc
     5161 gctggagaat cttcttcgga tcccggagca gagcgccctt gaaaaacgag agcgcgcagg
     5221 aatcgcggag cggaataatg atcgccacgt tcttcttctg gaaggtgaag cagggcttgc
     5281 tccacttccg ctcttcggtg aggaagcagt cgagggcgat cttgcggagc tgatccgtct
     5341 ccttctgcca cttcttcgca tagggaactt tcatgtgtcg agcttgcctc cctccatgtg
     5401 cgctgagctg accagccctg ccccacgacc tggtgtgctg tcccgaaacg caggggctga
     5461 agcccacatg atttttgcgc ctttgcggca cgactgaagt catgccctgt tacaaaagcg
     5521 ccggcccatt ctttaaggaa cttgcgggac accccactcg tgaggtgttt ccgaaatttc
     5581 ttcatgcgga agagaagcag gtccctccgc ttccccttcg ctacgctcag ggtccggttg
     5641 ggatgacaag gtaaaggaac ttcagaaaca ccccaccaga ttacagacgc cggtgtcgcg
     5701 ggcactcctc ttttctttct tctgaatcct ccgtgacttg ttttgtgtga tttgcgcctg
     5761 ggttcctttg ggagggtgcg gtatgtgatt gccgtgaggt catttgcgag gcgactgcgt
     5821 ttgattcatt cgcactcgtg tttgcgtcaa tggtgcacgt gacgctgccg gatgattgaa
     5881 atcgtgtcac ttgcatcgcg cgcgtgactg tatcaaacat gcgtgcgatt gatggggtca
     5941 cgagcgtcat tgattcactc gcgcgggtga ctgatgcatt ggcgattgcg acgcgggcat
     6001 gcccagagcg gcgagcatgg ccttgagttc gcgcatctcg gatggggatg cggggagcgg
     6061 attggcactg gcggcccgag gagagggtac cggcgtcgat gggggagcga gttcggggtc
     6121 aggaaacggg acatcgccga ggcggttgag gagggcgcgg atcaggcggc cggcggtaag
     6181 gtcggagatg tgcccggggg aggcgtcgcc gcggtc
//
LOCUS       DASZYN010000633         2895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1189360, whole genome shotgun sequence.
ACCESSION   DASZYN010000633 DASZYN010000000
VERSION     DASZYN010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189360"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VHE33_16195"
     CDS             complement(<1..407)
                     /locus_tag="VHE33_16195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99047.1"
                     /translation="MSVPVSDAVRSSTVYPWNPGGTGSVDAAALEVWVEDALQRHRQA
                     IELLQKAEGERSIDKTLRAYDDAVAALSTVGSLTGLMHSVHSDKAVRDTAQALLQKIS
                     QAGVELSLNRDVYQALAAIDASQADAATKHYLDR"
     gene            complement(486..1253)
                     /locus_tag="VHE33_16200"
     CDS             complement(486..1253)
                     /locus_tag="VHE33_16200"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HVT99048.1"
                     /translation="MLALVIPTLRESRNIRPLLSRVRAALDRYDCAYEVIVVDDASGD
                     GTEEIVTELAREDPRVRLIVRSGERGLAGAVLRGWEETEAQILAVMDADLQHPPELLP
                     ALWAELSGGADLAVGSRYADGGCMRSWKRVRRTISRIAIWMTLPVQRAGLRVHDPMSG
                     FFMVRRSCVEPISLQKTGFKILLEILARAEIRSVVEVPFKFGHRHAGASKANLRVALE
                     YVALLVRLYRQKGRTPIRAERELAPAALRSSEEAISG"
     gene            1365..2753
                     /locus_tag="VHE33_16205"
     CDS             1365..2753
                     /locus_tag="VHE33_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99049.1"
                     /translation="MKSCEPLRNFILPSLATAGAFLVEGYHPGVEDDAVYLSAIQRRL
                     DPGLYSHDFKWFTLQMQASIFDRMVAAWIRLTHMPVAWTELLGQYLAAFLLLWACLAV
                     SRRCFFTRAGQWAGVALIGVLFTLPVAGTALYIFDQYLHPRAIATVLVLWAILQILDR
                     RPLFAGVLLVSAVVMHPIMGVLGVSYCVILWIAGSRTGHTSVRSAPAMAMLSVAPLWI
                     FEGPSPAWRQALNTRDYYFIGRWTWYEWLGAIAPIFILWAIARYARRADRPSMALLSG
                     AGAIYAAFQLAVACIVLLTPALVRLTPMQPMRYLHLVYILMALLGGGLIGEKLLRGHV
                     WRWLVLFVPLAAGMFIAQRALFAGTTHLELPGIDARNPWLRAFAWVRANTPPTHTLRS
                     IRTTCRRAARTTTASAPWPSAANWPTQSKTQPSLRRSRIWRRYGWSRPSLSPAGSIFR
                     SRIFCGCSSDSA"
BASE COUNT          481 a          981 c          855 g          578 t
ORIGIN      
        1 cggtccagat aatgctttgt cgcggcatcg gcctggctcg cgtcaattgc cgccagcgcc
       61 tgatacacat cgcgattcag cgaaagctcc acgccggcct gcgaaatctt ctgtaacagt
      121 gcctgagccg tatcccgaac cgccttgtcg gagtggacgc tgtgcatcag ccccgtcagc
      181 gaacccaccg tggacagtgc ggcaaccgca tcgtcgtagg cgcgcagcgt cttgtcgatg
      241 cttcgctcgc cctcggcttt ttgcagcagt tcaatggcct ggcggtgccg ctgcaacgcg
      301 tcctcaaccc acacttccag cgccgcggcg tccaccgacc ccgtgcctcc gggattccag
      361 gggtagacag tactggagcg gacagcgtcg gaaaccggca cactcatgaa aaggcacctc
      421 tgttggcaga ctatcgcacc gtgctcagga tcgcagccgg acgattgtga aatgcgatgg
      481 gaagatcagc cgctgattgc ttcttcgctg ctgcgcaggg cggcgggggc cagttcccgc
      541 tccgcacgaa tcggcgtgcg gcccttttgc cggtagaggc ggaccagcaa cgcgacgtac
      601 tccagcgcca cacgcaggtt cgccttgctg gcgccggcgt ggcgatggcc gaacttgaag
      661 ggcacctcaa cgaccgagcg aatctcagct cgggccagaa tctccagcaa aattttgaac
      721 ccagtcttct gcagtgagat cggctcgacg caggagcgtc gcaccatgaa aaatccggac
      781 atcgggtcat ggacccgcaa tccggcgcgc tgaaccggca gcgtcatcca gatggcaatg
      841 cgcgaaatgg tgcggcgtac ccgtttccag ctgcgcatac agccgccgtc ggcataacgg
      901 ctgcccaccg ccagatcggc tccgccgctg agttctgccc aaagggccgg caaaagctcg
      961 ggcggatgct gcaggtcagc gtccatgacc gcaaggattt gcgcctcggt ttcttcccag
     1021 ccgcgcagca ccgcgccggc gagcccgcgt tctcccgagc gcacgatcag gcgaacgcgc
     1081 ggatcttcac gggcgagttc ggtgacaatc tcctcggtgc catcgccgct ggcatcgtcc
     1141 actacaatca cttcgtaggc gcaatcgtag cgatcaagcg cggcacggac ccggctcagc
     1201 aatgggcgga tatttcttga ttcgcggagg gtggggatga cgagcgccag catcggcgcg
     1261 catggattcg cctcgcatag cgccggtttt tcgccgctcg gtccctctaa agcgggtccc
     1321 cttgtgcgag agtccatgct gattgggaaa gacgccaaaa atccttgaaa agttgcgaac
     1381 ccctcaggaa cttcattctg ccgtcactcg caactgcggg cgcgttcctc gtggaagggt
     1441 accatcccgg cgtcgaagat gacgcggttt atctttccgc gattcaacga cgccttgatc
     1501 ccggccttta ttcccacgac ttcaagtggt tcacgctgca gatgcaggcc agcattttcg
     1561 accgaatggt ggccgcctgg atccgcctca ctcatatgcc ggtggcgtgg accgagctgc
     1621 tgggccagta tctggctgcg ttcctgctcc tctgggcctg cctggcggtg tcccggcgct
     1681 gctttttcac gcgggcagga caatgggccg gcgttgccct gattggggtg ctgtttacgt
     1741 tgcccgtcgc gggaaccgcc ctctacatct tcgatcagta cctgcatccg cgcgccatcg
     1801 cgacggttct ggttctatgg gccattctcc agatcctcga tcgccgccca ctattcgccg
     1861 gagtgctgct cgtctccgcc gtcgtgatgc accccatcat gggtgtgctt ggtgtctcgt
     1921 actgcgtgat cctgtggatc gcaggctcgc gcaccggcca cacctcagtc cgctcggcgc
     1981 ccgctatggc gatgctcagc gttgcgccgc tctggatctt cgaaggcccc tcgcccgcct
     2041 ggcggcaggc tctgaacacg cgcgactact acttcatcgg ccgctggacc tggtatgaat
     2101 ggctgggcgc catcgcgccc atcttcatcc tgtgggcgat tgcgcgctat gcccgccgcg
     2161 cggaccgccc atccatggcg ctgctctccg gcgcgggtgc catctacgcc gccttccagc
     2221 ttgccgtcgc ctgtatcgtc ctgctcacgc ctgcgctggt ccgcctcacg cccatgcagc
     2281 ccatgcgcta tctgcacctc gtctacatcc tgatggctct gctcggcggc ggcctgatcg
     2341 gcgaaaaact gctgcgcggt catgtttggc gatggctcgt gctgttcgtg cctctggccg
     2401 ccggcatgtt catcgcccag cgcgcgcttt tcgccgggac cacgcacctc gagctgcctg
     2461 gcatcgacgc gcgcaatccc tggctgcgtg ccttcgcctg ggttcgcgcg aatacccccc
     2521 cgacgcatac tttgcgctcg atccgtacta catgcaggcg cgcggcgagg actaccacag
     2581 cttccgcgcc ctggcccagc gcagcgaact ggccgacgca gtcaaagacg cagccgtcgc
     2641 tacgcaggtc ccggatctgg cgccgctatg ggtggagcag acccagcctc tcaccggctg
     2701 gcagcatttt cagaagcagg attttctgcg gctgcagcag cgattcggcg tgaactggat
     2761 cgtcgtccag ggttccggta tgcccgggct cgactgcccg tacaaaaacg aggccgtcgc
     2821 cgtctgccat ctgcaataag atccagcgtc tgttagagaa tcttctttcc aaacgccgag
     2881 gaaatcagct ccacc
//
LOCUS       DASZYN010000634         6127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_484012, whole genome shotgun sequence.
ACCESSION   DASZYN010000634 DASZYN010000000
VERSION     DASZYN010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484012"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VHE33_16210"
     CDS             <1..330
                     /locus_tag="VHE33_16210"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVT99050.1"
                     /translation="GVAGVLAFSVSGRTREFGIRMALGAEPRHILTDVLREGLIIASI
                     GVVSGLVVGLAGAKGLAAWIGQIHLPGPLTFIAAAAVILASAVIASAVPAARAARVNA
                     VEALRAE"
     gene            complement(398..2497)
                     /locus_tag="VHE33_16215"
     CDS             complement(398..2497)
                     /locus_tag="VHE33_16215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004629 - phospholipase C activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase C, phosphocholine-specific"
                     /protein_id="HVT99051.1"
                     /translation="MPTNRRSFLKLAASGALSASFPASIARALEIPANDRTRSIADVE
                     HIVILMQENRSFDHYFGTLRGVRGFGDPRAVRLPNGDPVWKQPDGAGGYVLPFHPDLT
                     NAGLAFVEDMPHDWDTTHDAFNNGAYDQWVPQKGTTGMTHLNRQDIPFHYALADAFTI
                     CDAYHCSLLGPTDPNRYHMWTGWVGNDGSGGGPVVDNAEAGYGWSTYPERLSAAGVSW
                     KIYQDIGDGLDGPDYWGWTGDPYIGNYGDNSLLYFHQYQNAAPGSPLYEGARMGTNTK
                     VSGNLVDQFRADVAANKLPQVSWIVAPEAYSEHGNWPPNYGAWYTTQFLDALTANPQV
                     WSKTVLLLTWDENDGFFDHVVPPTPPQTRAQGISTVPTTNEIFPGNASYEAGPYGLGV
                     RVPMLVISPWSKGGYVSSEVFDHTSIIRFIEARYAGHHRKLIETNITPWRRAVSGDLT
                     SAFNFRNPNASIVTLPNTDAYAPPAADIVAGTRYPDYVPPVPANQTMPIQEPGTKPAR
                     PVPYELFVHGEANFADGTFGIRFVNRGKTAVLQVRSGNSSLGPWTYTVGSGEEVSDTW
                     PITGNSLTGYDLSVYGPNGFLRAFRGSVGGAEKANLDIRAIPDRDGDSITLWGANRGS
                     QAVTLQMTDGYSKKTWSYDLAAGAEVSGAIALADTFGWYDFIVEVEQDLTFRYEIAGH
                     AESERESRTDPAIGATA"
     gene            2688..3530
                     /locus_tag="VHE33_16220"
     CDS             2688..3530
                     /locus_tag="VHE33_16220"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HVT99052.1"
                     /translation="MPCDPVELRHVPLFELLDDDEAAVLAAQVDVRHFAPRQRIFKAG
                     DPSGNAYIVMTGSVRVFITDEDHQEVTVDEPTHGEAFGMASMMRQVEHQTSAVALEEC
                     TCLEIGRDDIAVLLTRRPMAGLDMLTMQARQFHTSQQLVRSRANRNPNEVIEEQETFG
                     ERIADAVARFGGSWAFIITFAVVLIAYSSTNIVLKGRAWDPYPFILLNLFLSMLAAIQ
                     APVIMMSQNRQDVKDRLRSELDFEVNRRAESEIQSLSQRLSLITDKLEDVEDLIRKAP
                     TAPL"
     gene            complement(3556..4038)
                     /gene="bfr"
                     /locus_tag="VHE33_16225"
     CDS             complement(3556..4038)
                     /gene="bfr"
                     /locus_tag="VHE33_16225"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HVT99053.1"
                     /translation="MTGMKGNAKVVERLNEALRDELTAINQYFLHSEMAENWGYDKYS
                     KFIKKQSIDEMRHAEALMERILFLDGTPSMEPLGLTIGRNVKEMIESDLKLELGAVAS
                     YNESIRICVENKDNGSRDLFVKLLKDEEGHVDWLEAQVHKIGEIGYERYLITQSEEEE
                     "
     gene            4152..5360
                     /gene="meaB"
                     /locus_tag="VHE33_16230"
     CDS             4152..5360
                     /gene="meaB"
                     /locus_tag="VHE33_16230"
                     /inference="COORDINATES: protein motif:HMM:NF015279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HVT99054.1"
                     /translation="MTATAISDLVARLRAGDTRALARAISLVENDAPEAPALLSACFP
                     HTGRALRIGVTGAPGAGKSTLVDRLARAFRDAGKSVGVVAVDPTSPFTGGAILGDRIR
                     FREHQTGVPGESARHSDPGFYLRSMATRGSLGGLALATADVALVLEASGKDVLLIETV
                     GVGQDEIEIARLADITLVILVPGMGDDVQSLKAGVMEIADIFVINKSDRDGADRVEQE
                     IRGLQSLAPATRSPARFELEDHERDSASLPGRHSTRHPQTAFGSEHDSTGALHAQTAF
                     VSGHDFSRAEMAPGEKGALAPEAVPTPPVIRTVATTGDGLPALTSAIAALEQWLRDDK
                     RLAARRTAHWQRRLDAMLRTELMRRARQNGFDAKVLQFHAARIASGQQDPWQLVAQLT
                     EKVFNPEKSG"
     gene            5411..5818
                     /gene="mce"
                     /locus_tag="VHE33_16235"
     CDS             5411..5818
                     /gene="mce"
                     /locus_tag="VHE33_16235"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HVT99055.1"
                     /translation="MPELDHLGIAVKSIDQARKLYESLGLQVVHEETVDHEHVRTALI
                     PAGDARIELLEPTSPDSPVGRFLAKRGEGLHHIALHVEDISGTLETLKAQGARLISED
                     IQTGAGGHLYFFVHPSSAGGVLLEICQDGVSDV"
     gene            5886..>6127
                     /gene="tsaB"
                     /locus_tag="VHE33_16240"
     CDS             5886..>6127
                     /gene="tsaB"
                     /locus_tag="VHE33_16240"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HVT99056.1"
                     /translation="MPLLSIDTCGATGTVALARLADDKIALLAQAELPGKTYSARLVS
                     TIRDLLAANDLQLAALEAIVVTTGPGSFTGIRIGLST"
BASE COUNT         1118 a         2269 c         1559 g         1181 t
ORIGIN      
        1 ggcgtcgccg gcgtacttgc cttctccgtc agcggacgca cccgtgagtt cggcatccgc
       61 atggccctgg gcgcagagcc gcgccacatc ctcaccgacg tactccgcga aggcctcatc
      121 atcgcctcca tcggagtcgt ctcgggcctg gttgtaggtc tcgccggagc caagggcctc
      181 gccgcatgga tcggccagat tcacctgccc ggcccgttga ccttcatcgc cgccgccgct
      241 gtcatcctcg cgtctgccgt aatcgcctca gccgtgcccg cagcccgcgc cgcgcgcgtc
      301 aacgccgtcg aagccctccg cgccgagtag cgcaacccgg acaaacaaag ggtccccact
      361 caaaccgagt ggggacctgt ccttcttcct tgatcttcta cgccgtcgct ccaatcgccg
      421 gatcggtccg gctctcccgc tcgctctccg catggccggc gatctcatag cggaacgtca
      481 gatcctgttc gacctccact ataaagtcgt accagccaaa cgtatccgca agcgcgatcg
      541 cccccgacac ctctgcaccg gccgcaagat cgtagctcca cgtcttcttc gaatatccat
      601 cggtcatctg cagcgtcacg gcctgcgagc cgcggttcgc gccccacaga gtaatcgagt
      661 caccgtcccg atcgggaatg gcgcggatat ccaggttcgc tttctccgcc cctccaacac
      721 ttccccgaaa cgcccgcagg aatccgttcg gcccgtacac cgacaggtca taccccgtga
      781 gactgttgcc cgtgatcggc caggtgtcgg aaacttcctc gccggaccca accgtgtacg
      841 tccatggccc caggctgctg ttgcctgagc gaacctgcag aaccgcggtt ttgccccgat
      901 tcacaaatcg aatcccaaac gtcccgtccg caaaattcgc ctcgccatgc acaaacagct
      961 catacggcac aggccgcgct ggcttggtgc ctggttcctg aatcggcatc gtctggttcg
     1021 ccggcaccgg cggaacataa tccgggtatc gcgtgcccgc aacgatgtca gctgccggcg
     1081 gagcataggc gtccgtgttg ggcagcgtca caatgctggc gttcggattg cgaaagttga
     1141 atgccgaagt cagatcgccc gaaaccgcgc gccgccacgg tgtgatgttt gtctcaatca
     1201 gcttccgatg gtgcccggcg taacgcgctt cgataaaccg aatgatggaa gtgtggtcga
     1261 acacctctga gctcacatat ccccccttgc tccatggaga gatcaccagc atcggcaccc
     1321 gcacgcccag tccatacggt cccgcctcgt agctggcgtt ccccggaaag atctcgttcg
     1381 tcgtcggaac cgtcgaaatc ccctgcgctc gcgtctgcgg cggagtcggc ggcaccacgt
     1441 gatcgaaaaa cccgtcgttc tcatcccagg tgagcagcaa caccgtcttg ctccagacct
     1501 gaggattcgc cgtcagcgcg tccagaaact gcgtcgtata ccacgctccg taattcggcg
     1561 gccagttccc gtgctcgctg tatgcctccg gcgccacaat ccacgaaacc tgtggcagtt
     1621 tgttcgccgc gacgtcggcc cggaactgat ccaccaaatt gcccgacact ttcgtattcg
     1681 tgcccatccg cgcgccctca tacagcggac tgcccggcgc cgcattctga tactggtgga
     1741 aatagagcag cgaattatcc ccgtagttcc cgatgtaagg atcgcccgtc cacccccagt
     1801 aatccggtcc gtccagccca tcgccgatgt cctgataaat cttccacgac acacccgccg
     1861 cactcagccg ttccggatag gtgctccagc cgtagccggc ctccgcgttg tcgacaaccg
     1921 gcccgccgcc gcttccatcg ttgcctaccc aacccgtcca catgtgatac cgattcggat
     1981 cggtcgggcc cagcagcgaa cagtggtacg cgtcgcaaat cgtaaacgca tccgccagcg
     2041 cgtagtggaa tggaatgtcc tgacggttca gatgcgtcat cccggttgtt cccttttgcg
     2101 gaacccactg gtcgtacgcg ccgttgttga acgcgtcgtg cgtcgtgtcc cagtcatgcg
     2161 gcatgtcttc cacaaacgcc agccccgcat tcgtaagatc gggatggaac ggcaaaacat
     2221 atcctcccgc gccgtccggc tgcttccaca ccgggtctcc attcggcaac cgcaccgcgc
     2281 gcggatcgcc gaaccctcgc acgcctcgca gcgttccgaa gtagtgatcg aaggaacggt
     2341 tttcctgcat caggatcacg atgtgctcta catccgcgat gctccgtgtc cggtcgttag
     2401 ccgggatttc cagagcgcga gcaatgctgg ctgggaaaga tgcggaaagc gcgccggagg
     2461 cggcgagctt taaaaacgag cggcgattgg ttggcatggg gacgctcctc gaagatcacg
     2521 ttcgcagacc accggagctt agccccgcaa tcattgcgat tgcatgattg cccggttgga
     2581 attctgtgac tcgccgcgga tatctggatc taaaacggtg acaatgtcct catcccgtct
     2641 gctaccctcg cttccacaga ctgtcacctc atccccgagg cccatccatg ccctgcgacc
     2701 ccgtagaact ccgccacgtt cccctgtttg aattgctcga cgacgatgaa gccgccgtcc
     2761 tcgctgctca ggtcgatgtg cgccacttcg ccccgcgcca gcgcatcttt aaagccggcg
     2821 atccttccgg aaacgcctac atcgtcatga ccggcagcgt ccgcgtcttc attaccgatg
     2881 aagatcacca ggaagtgacg gtcgacgagc ccacccacgg tgaggctttc ggcatggcgt
     2941 ccatgatgcg ccaggtcgag caccaaacct ccgccgtcgc tctggaagaa tgcacctgcc
     3001 ttgaaatcgg ccgcgatgac atcgccgtcc ttctcacccg ccgccccatg gccggcctcg
     3061 acatgctcac catgcaggcc cgccagtttc atacttctca gcagctggtc cgcagccgcg
     3121 ccaaccgcaa tcccaacgag gtgattgaag aacaggagac cttcggtgaa cgcatcgccg
     3181 acgccgtcgc ccgcttcggc ggctcctggg ccttcatcat taccttcgcc gtcgtgctca
     3241 tcgcttactc gtccacgaat atcgtgctca aaggccgcgc ctgggatccc taccccttca
     3301 tcctgctcaa cctcttcctc tccatgctcg cggccattca ggcaccggtc atcatgatga
     3361 gtcagaaccg ccaggacgtg aaggaccgtc tccgcagcga actcgacttc gaagtcaacc
     3421 gccgcgccga atccgagatc cagtccctct cccagcgcct cagcctgatc accgacaaac
     3481 tcgaggacgt cgaagacctg atccgcaaag cgccaacggc tccgctctga tccttcattg
     3541 gctactggct actggctact cttcttcttc gctctgcgta atcaggtagc gctcgtagcc
     3601 gatctcgccg atcttgtgca cctgtgcttc cagccagtcc acgtggcctt cctcgtcttt
     3661 gagcagcttc acaaacagat cccgcgagcc gttgtctttg ttctccacgc agatccggat
     3721 cgactcgttg tacgacgcca ccgcccccag ctccagcttc aggtctgact cgatcatctc
     3781 cttcacgttc ctcccgatcg tcagccccag cggctccatg ctgggtgtcc cgtccaggaa
     3841 caggatccgc tccatcagcg cctccgcgtg gcgcatctcg tcaatggact gcttcttgat
     3901 gaatttcgaa tatttgtcgt agccccagtt ctccgccatc tcggagtgga ggaagtactg
     3961 gttgatcgcg gtcagttcat cgcgaagcgc ctcattcagc cgttcgacaa cttttgcgtt
     4021 acccttcatg ccggtcattt tacagaatga ctccggcatt gcgcccctgt aatctcccgc
     4081 cgagaaatgg cgttcagccc cgcacagaaa ggcagcgccc gtcgtccgtc acgaggcgct
     4141 atcctgattc cttgaccgcc accgcgatct ccgatctggt cgctcgcctc cgcgccggcg
     4201 acacccgcgc cctcgcccgc gccatctccc tggtggaaaa cgacgctccc gaagcccccg
     4261 cgctcctctc cgcctgcttc ccccacaccg gccgcgctct ccgcattggc gtcaccggcg
     4321 ctcccggagc cggcaagagc accctcgtcg atcggctcgc acgtgcgttc cgcgatgccg
     4381 gcaagtctgt cggcgtcgtc gccgttgacc caaccagccc cttcaccggc ggcgccatcc
     4441 tcggcgatcg catccgcttt cgggagcacc aaacgggtgt tcccggcgaa tccgcccgtc
     4501 attccgatcc gggcttctat ctccgctcca tggccacccg cggctctctc ggcggactcg
     4561 ccctcgctac cgccgacgtt gccctcgtcc tcgaagccag cggcaaagac gtgctcctca
     4621 tcgaaaccgt cggcgtcggc caggacgaaa tcgaaatcgc ccgcctcgcc gacatcaccc
     4681 tcgtcatcct tgtccccggc atgggtgacg acgtccagag tctcaaagcc ggcgtcatgg
     4741 aaatcgccga catcttcgtc atcaataaat ccgatcgcga cggcgccgat cgcgtcgaac
     4801 aggaaatccg cggcctgcaa tcgctggctc ccgcaacgcg ttcgcccgct cgatttgaac
     4861 tggaggatca cgaacgagat tctgcttctc taccagggcg ccactcaacc cgccatcccc
     4921 aaactgcttt tggatcggag cacgattcaa ctggagcgct ccatgcccaa accgcctttg
     4981 tatcagggca cgacttcagt cgtgccgaaa tggcgcccgg agagaaaggg gctttagccc
     5041 ctgaagccgt cccgaccccg cccgtcattc gcaccgtcgc caccaccggc gatggcctcc
     5101 ccgccctcac ctccgccatc gccgccctcg aacaatggct ccgggatgac aaacgcctcg
     5161 ctgcccgccg aacagcccac tggcaacgcc gcctcgacgc catgctccgc accgagctca
     5221 tgcgccgcgc ccgtcagaac ggtttcgacg cgaaggtgct tcagttccac gccgcccgca
     5281 tcgcctccgg acagcaagac ccctggcaac tcgtcgccca gctgaccgag aaggtcttta
     5341 acccagaaaa gtcgggctga gcagcgtcag cccgctattg gcgattggct attccctata
     5401 ccctgtaggt atgcccgaac tcgaccacct cggcatcgcc gtcaaaagca tcgaccaggc
     5461 ccgcaagctc tacgaatccc tcggcctcca ggtcgtccac gaagaaaccg tcgatcatga
     5521 gcacgtccgc accgcgctga tccccgccgg cgatgcccgc atcgaactcc tcgagcccac
     5581 cagccccgac tcgcccgtcg gccgctttct cgccaaacgt ggcgaaggtc tccaccatat
     5641 cgccctgcac gtcgaagaca tctccggcac cctcgaaacc ctcaaagccc agggcgcgcg
     5701 cctcatctcc gaagacattc aaaccggtgc cggcggccat ctctactttt tcgtccatcc
     5761 ctccagcgcc ggcggcgtgc tcctcgagat ctgccaggac ggagtgtcgg acgtctaagc
     5821 gggacagatc cagagccgga cagctcttcc agacgctgta cgctaaacgc taaacgctgc
     5881 cctttatgcc cctcctctcc atcgacacct gtggcgcaac cggcaccgtc gccctcgctc
     5941 gcctcgctga cgacaaaatc gccctcctcg cccaggccga actcccaggc aagacgtact
     6001 ctgcccgcct cgtctccacc atccgcgatc ttcttgccgc aaacgacctc cagctcgccg
     6061 ctctcgaagc catcgtcgtc accaccggcc ccggctcctt caccggcatc cgcattggcc
     6121 tctccac
//
LOCUS       DASZYN010000635         4436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1059918, whole genome shotgun sequence.
ACCESSION   DASZYN010000635 DASZYN010000000
VERSION     DASZYN010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059918"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /gene="bamD"
                     /locus_tag="VHE33_16245"
     CDS             complement(<1..353)
                     /gene="bamD"
                     /locus_tag="VHE33_16245"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HVT99057.1"
                     /translation="MNRLGIRISAVCLGMAIVLSSPSAFAREHAKKKKKNVDLSANPL
                     GNVKSSQPDKLLFDKAAHAMKHGKYDVARLDLQTLLNTYPDSEYQMRAKLMVGDTWFK
                     EGGTAALTQAESEYK"
     gene            complement(436..1113)
                     /gene="rpe"
                     /locus_tag="VHE33_16250"
     CDS             complement(436..1113)
                     /gene="rpe"
                     /locus_tag="VHE33_16250"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HVT99058.1"
                     /translation="MVELVPSILSADFARLADEIAAAERGGGTVIHVDVMDGHFVPNI
                     TVGPPIVASLRKHTKLPLDCHLMIENPDEFIPAFAEAGASWVSVHYEACRHLHRSLQL
                     IAQHNMQPAVVINPHTEVDLLIPVLGMVHHVLVMSVNPGFGGQRFIPYSLNKIRRLAE
                     LRRQLGLSYRIEVDGGVAHDTVSSIVEAGAELLVAGNAVFGNGNAERDARSLLDAAQR
                     AASVAVA"
     gene            complement(1169..2122)
                     /locus_tag="VHE33_16255"
     CDS             complement(1169..2122)
                     /locus_tag="VHE33_16255"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HVT99059.1"
                     /translation="MPPVLKEDRLRAAAAHGRHHAGHGIGYLACALAGCCWGMGFFFG
                     KIAFTRLGVGHYVLYRFVFACLGLLPIVEAPKFSAREWRLLLIGAFLGVPFQFMVQFA
                     GLRLTTVSHAALMVGTMPVILAVGATIFAHERLDLKGWIALLGSTAGIALIVLSAGHG
                     AIRGGSIRGDLLVVLSLVIALGWVLINQRLMVLGRSPLAVSSWTLLTGSAMLFPWVIL
                     QDGLPPVRGLGWHVWFAVTASGLLCTSAATLLWNWGMHRVPASRAGVFLNIEPALGSI
                     LGVVVLGEHLGPGAYAGGALIIAAVVVLTMTGQRVPHAESA"
     gene            complement(2181..2822)
                     /locus_tag="VHE33_16260"
     CDS             complement(2181..2822)
                     /locus_tag="VHE33_16260"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HVT99060.1"
                     /translation="MLLYAITNRRLLPGDENGQRDALVALASDWAQGGVDTIQVREKD
                     LPLNELQVLTARIVESVRATKSRTRVLVNGPAQVALDAGADGVHLHANAGAAAIRAAQ
                     QAYARNGQHPVISAACHNSDEIRAAAGASMLLFSPIFEKVTVQGITLGKGLATLRAAV
                     DLANPTPVLALGGVTEKNAAACVQNGAAGIAAIRLFLGDGWRALLDVPVRVSA"
     gene            2930..4327
                     /gene="sthA"
                     /locus_tag="VHE33_16265"
     CDS             2930..4327
                     /gene="sthA"
                     /locus_tag="VHE33_16265"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HVT99061.1"
                     /translation="MSKFDLLVIGSGPSGQRAAVSAVKKGKSVALVEMRSVVGGVCIN
                     TGTIPSKTMREAVLHLSGYNYRSIYGMNYRVKEKITMADLAFRVQHVVKTEIDVTEAQ
                     LSRNGVEVFNGVARFEDPHHVRVDGPRGTNTYEADNVIIAVGTRPASSAKVPVNGKTI
                     LNSDQVLDMLELPKSLIVVGGGVIGVEYTCMFAALGVRVILIEKRPRLLEFADQEIIE
                     ALSYHLRDSRVTMRLGEEVESVEQLADGTVVANLESKKKMSGDALLYAVGRQGNIDDL
                     NLQAAGIDADNRGRIPVDKDFRTKVPHIYAVGDVIGFPALASVSMEQGRIAAARAFGD
                     DHIISNPSFYPYGIYTIPEISFIGKTEEQLTEEDVPYEVGVAYYREIARGQIRGDTTG
                     RLKLVFHRETRQVLGVHIIGEGAAELVHIGQAVMALNGNVDYFIDTVFNYPTLAECYK
                     AAAFNGINRLARFEG"
BASE COUNT          946 a         1412 c         1335 g          743 t
ORIGIN      
        1 tccttgtatt cagactctgc ctgggtgagc gccgccgtgc cgccttcctt gaaccaggta
       61 tcgccaacca tgagcttggc tcgcatctgg tattcggagt cgggataggt gttgagcagg
      121 gtctggagat cgagccgggc cacatcgtac ttgccatgct tcatggcatg ggcggcctta
      181 tcgaagagca gcttgtccgg ctgcgacgac ttcacgttgc ccagcgggtt ggcgctgaga
      241 tcgacgttct tcttcttttt cttcgcgtgt tccctggcga aggccgacgg agaagaaagc
      301 acgatcgcca tgcccagaca aactgccgaa atacggatac ccaaccgatt cataacacct
      361 caatagtcgc aaacatctcg tgcgatcgtg cgccggtacg gggtggtctc cacggacccg
      421 aaccagacca aagacttaag ccacggccac gcttgcggcc cgttgcgctg cgtcgagcag
      481 gctgcgagcg tcgcgttcgg cgttcccgtt accgaagacg gcgttgccgg caaccaacag
      541 ctcggctccc gcttcgacga tgctggagac ggtgtcgtga gcgacgccgc catccacttc
      601 aatcctataa ctgaggccga gctggcgtcg aagctcagcg agccggcgaa tcttgttgag
      661 cgagtacggg atgaaccgct ggccgccgaa gcctggattc acgctcatca cgagcacatg
      721 atgcaccatg cccaggactg gaatgagcag atcgacttcg gtgtggggat tgatgacgac
      781 ggcgggctgc atgttgtgct gcgcgatgag ttggagcgag cgatgcaggt gccggcaagc
      841 ctcgtaatgg acgctcaccc agctcgcgcc cgcctcggcg aacgccggga taaactcgtc
      901 cggattctcg atcatcaggt ggcagtcgag aggcagcttg gtgtgcttgc gcagcgaggc
      961 tacgatgggc gggccgaccg tgatattagg aacaaaatga ccatccatca cgtcgacatg
     1021 gatcacggtg ccgcccccgc gctcggccgc ggcaatctcg tcggccagcc gcgcaaagtc
     1081 ggcggaaagg atggatggaa ccagttcaac cactaagaaa cagggtacag gagacagggg
     1141 gcagggtaca ggggaggaat gcggacagtt aggccgactc ggcatgcgga acgcgctggc
     1201 cggtcatggt cagcaccacc accgccgcga taataagcgc cccacctgca taggctcccg
     1261 ggcccaggtg ctcacccagc accacaacgc ccaaaatcga cccgagcgcc ggctcaatgt
     1321 tcaagaacac gcccgcccgc gacgctggca cgcgatgcat gccccagttc cacagcaaag
     1381 ttgcagccga cgtgcacagc agtccactgg cggtgacggc gaaccaaaca tgccatccca
     1441 ggccgcgcac cggcggcaat ccgtcctgca gaatgaccca gggaaacaac atggcggagc
     1501 ccgtgagcag agtccaggag ctcaccgcaa ggggcgagcg gcccagcacc atcagccgtt
     1561 gattgatcag gacccacccc agggcgatca ccagcgacag gacgaccaac agatcaccgc
     1621 gaatacttcc cccccggatc gcgccgtgac cagcgctcag cacgatcagc gcaatgccgg
     1681 ccgtcgagcc cagcagcgcg atccaaccct tcagatcgag ccgctcgtga gcgaaaatcg
     1741 tcgccccaac cgcgagaatc acgggcatcg tgccaaccat gagcgcggcg tggctcaccg
     1801 tcgtcagcct gagccccgcg aactgcacca tgaactggaa aggaacaccg agaaacgcgc
     1861 cgatcagcag cagcctccac tcgcgagcgg aaaacttcgg tgcctcgacg atcggaagca
     1921 ggccgaggca cgcaaacaca aaccggtaga ggacataatg cccgacaccc agtcgcgtga
     1981 aggcgatctt gccaaagaaa aaccccatgc cccagcagca gccggcgagc gcgcaggcga
     2041 ggtagccaat gccgtgccca gcatggtgac ggccatgggc cgctgcggcc cggaggcggt
     2101 cttctttcag cactggcggc atttcgcggt tggatgatcc atcagaacga tggtcgcacg
     2161 gatgtgacga aagccagcag ctacgccgat acgcgcacgg ggacatcgag aagggcccgc
     2221 cagccatccc ccagaaagag ccgaatcgcg gcgatgcccg ccgcgccatt ctgcacgcac
     2281 gcggcagcat tcttttccgt aacgccgccc agggccagaa ccggagtcgg gttcgcgagg
     2341 tcgacggcgg cacgcagggt tgcgagtcct ttgccgaggg tgattccctg tacggtgacc
     2401 ttttcgaaaa tcggggagaa cagcagcatc gacgcccctg cggccgcacg aatctcgtcg
     2461 gagttgtgac aggcggcgct gatcacggga tgctgaccgt tgcgcgcata ggcctgctgt
     2521 gcggcgcgga tcgcagcagc tccggcgttg gcatgaaggt gcacaccatc ggcgcccgca
     2581 tcaagagcaa cctgcgccgg cccattcacc aaaacccgtg tcctgctctt cgtcgcgcga
     2641 acagactcaa caatccgagc cgtcagaacc tggagttcgt tcagaggaag gtccttttcg
     2701 cgaacctgga tcgtgtccac cccgccctgt gcccagtcgc tggcgagcgc taccagcgcg
     2761 tcgcgctgcc cattttcgtc gcccggcagc agacggcggt tcgtgatggc atagagaagc
     2821 atcagaagtt cagtgtaagc gtggattcat acggcctgtc atcaatggaa tgtaagatag
     2881 gctctgcgcc ggcaccggac tgacttccga tgcaggccat ttctgacaca tgtcgaaatt
     2941 cgatctgctc gtcatcggtt ccggcccttc cggccagcgc gcggcggttt ccgcggtaaa
     3001 gaagggcaaa agcgtcgcgc tggttgagat gcgcagcgtc gtcggcggcg tgtgcatcaa
     3061 caccggcacc atccccagca aaaccatgcg cgaagccgtg ctgcatcttt ccggctacaa
     3121 ctatcgctcg atttacggca tgaattaccg cgtgaaggaa aagatcacca tggccgatct
     3181 agcctttcgc gtgcagcatg tggtgaaaac cgaaatcgat gtgaccgagg cgcagctttc
     3241 ccgcaacggc gtggaagtct tcaacggggt cgcccgcttc gaggatcccc atcacgtaag
     3301 ggtggacggc ccgcgcggga ccaacaccta cgaagcggac aacgtcatca tcgccgtggg
     3361 cacccggccg gcatcgtcgg cgaaggttcc ggtgaatggc aagacgattc tcaacagcga
     3421 ccaggttctc gacatgctgg agttgcccaa gagcctcatc gttgtcggag gcggcgtcat
     3481 cggcgtcgaa tacacctgca tgtttgccgc gctcggcgtg cgcgtcatcc tgatcgagaa
     3541 acggccgcgc ctgctcgagt ttgccgacca ggaaatcatc gaggcgctca gttatcacct
     3601 ccgcgactcg cgcgtgacca tgcgcctggg tgaggaagtg gaaagcgtgg agcagctcgc
     3661 cgacggcacc gtcgtcgcca atcttgagag caagaaaaaa atgtccggcg acgccctgct
     3721 ctatgccgtc ggacgccagg gcaacatcga cgacctcaac ctccaggccg ccggcatcga
     3781 cgccgacaat cgcggacgca tcccggtcga caaggatttc agaaccaaag tgccgcacat
     3841 ctacgcggtc ggcgacgtca tcggcttccc tgccctggct tcggtttcaa tggagcaggg
     3901 acgcatcgcc gcagcacgcg cctttggcga cgaccacatc atttccaacc ccagcttcta
     3961 tccctacggg atttacacca tcccggagat ttccttcatc ggcaaaaccg aagagcagct
     4021 caccgaggag gacgtgccct acgaagtcgg cgtcgcctac tatcgcgaga tcgcgcgcgg
     4081 ccagattcgc ggcgatacga ccggccgtct caagctggtc ttccatcgcg aaacccgcca
     4141 ggtgctcggc gtccacatca tcggcgaagg agctgccgag ctcgtccaca tcggccaggc
     4201 tgtcatggct ctcaacggca acgtggacta cttcatcgat acggttttca actatcccac
     4261 cctcgccgag tgttacaagg ctgccgcgtt taacggcatc aaccggctgg cccgctttga
     4321 agggtgagcc gcgagcgcgg aggagttatg tccaggacag taggcgcggt gatgacgacg
     4381 cgggtcgtcg cgggacttat cgaagatcac aagctccagg gcagcctgaa ggaatt
//
LOCUS       DASZYN010000636        14701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_355199, whole genome shotgun sequence.
ACCESSION   DASZYN010000636 DASZYN010000000
VERSION     DASZYN010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 14701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14701
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355199"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(528..1241)
                     /locus_tag="VHE33_16270"
     CDS             complement(528..1241)
                     /locus_tag="VHE33_16270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT99062.1"
                     /translation="MSQTVFVLEDDADIARLIQHHLEAAGFPARIYATPASLIPDAER
                     QAPALFLLDIMVPGGDGLDVCRRLRNHPALSSIPIIFVTARAAENDRVLGLELGADDY
                     ITKPFAPRELLARVKAVLRRFERPSTPSAIAFEDVEIDASAMQLRVRGSLTTTTATEF
                     RLLDYLARHPGRVFSRDHLLDAVWGDARFVTPRSVDVYVRRIREKIESDPENPRYLKT
                     VRGAGYRFEIPRVAERAAV"
     gene            1545..1940
                     /locus_tag="VHE33_16275"
     CDS             1545..1940
                     /locus_tag="VHE33_16275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT99063.1"
                     /translation="MPIPTILCIDDEALGLEIRKAVLERAGYQVLTAVDGATGLTLFR
                     GNPVDGVVLDYYMPEMNGGAVAEAMRRERPEIPIMLLSAYINLPTDVVQLADVTVLKG
                     EGPQELLEKLRAMLHSAGMAGGARAEGSA"
     gene            1937..3718
                     /locus_tag="VHE33_16280"
     CDS             1937..3718
                     /locus_tag="VHE33_16280"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HVT99064.1"
                     /translation="MKELQFNRTLRQLVALPIILLVFMAGFLVWQIAAASIAQKALDH
                     SDEVTAQLQELQNLFVDQETGLRGYQLTGDPVMLEPWKSAAGPIQNHFATLRRMLAGD
                     SEQSKRLEDLQILYNQWLGFANAVLHQDAASLNAAGMNQRGKAMMDGIRSATADMVGH
                     EANLRAVRSQHARWMQRRELVSLLVSAVVVGLFLALFTRHHVHAISASYQRRIAEITK
                     QSNELFESQQWFRTTLESIGDAVIACDYRGAVSFMNDVAEKLTGWTSSEAKGKPLAKV
                     FHIVNEQTRKVCENPVDKVRRLKKVVGLSNHTVLVGRGGREYVIDDSAAPIMNDAAKM
                     IGVVLVFRDVTEERQVQAALLASEKLAVAGRLAASIAHEIHNPLDSVANLHYLLAQES
                     DPAKREEYLRLAQQELGRTMQISRTMLSLYREPRSPIQIDLRELIEGVLLLLDRRLQN
                     QGIVLETKIAGDLSVEGFPAELRQVFTNLIVNAVEAAGAKGKVRITMERAESAEMHGA
                     GAAIEIADSGPGIAPEAAKRLFQPFFTTKGADGTGLGLWVSMGIVQKHGGSISVVESS
                     DPELPGACIRVFLPARTMAQRAGEPAA"
     gene            complement(3730..6204)
                     /locus_tag="VHE33_16285"
     CDS             complement(3730..6204)
                     /locus_tag="VHE33_16285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT99065.1"
                     /translation="MSGAWRDVRLALRQMRKAPGFYLTAILTLALGMGATIAIFSLVE
                     GILLRPLPFADPARLVIVGDHLGNNPNPGVTAREIATYERATTAFSSMGGFAGAGYEI
                     SGAATAQLIAGARLGAGVFSALGVSPELGRVFTKQEDDGHTPVAVISDALWLNRYHRD
                     PHVIGQAIVLDRKSYSIVGVMPPSFEFPLSAGRMNKAQVWVPLSLTAEELSDKNVGAW
                     MYRMVARVKDGVTLQQAAEDAHRVSQQIMRDFPPTMAAIRIRGDALSLREQSVAATRP
                     VLRALFVAVLIVLAIACANVAGLLLVRAIGRRREYAVQAALGAPPRAILRQSIVAGLI
                     LGLAGGALAVGLSAVAVRTVLRLLPDSLPRVDTIAIDPAVLIFALLLALLTGALCSAA
                     PAFAATRASVIDNLKEAGRSGTGSLSHSWLRSFLVVAEIATALVLLTTAGSFLRSYQK
                     MLAVNPGFQPEHVLAASYQLPLEQYSTQTQVENFTHAVIDRLVAKPGVVAAGFTNSLP
                     TSNNGGLAAYTVEGQPAATWKLTFAAFVITGGDYFRTMGIPLLAGRYFTPQDRADAPL
                     VIIVNEAMAKHCWPGQSALGKAIHVGNPQKGYPWATVVGVVGNTVLGPRDAPVADQWY
                     MPAEQPSILYGSAAKQNLVQAAGGFIALRSSLDPDQMKQVLRSSVAEVDPQLALDQVE
                     TMRDSMANVEAPRRFNTDLMTSFAAAALLLALTGIYAVVAFSVTLRSREIAIRMAMGA
                     QRASIARMVLLSGAKLAFAGCTLGVLGSLAVSHLLVAFLFGVSATDPLIYTVTIATMF
                     LISILAAALPANRAAGTDPVEALRAV"
     gene            complement(6325..7266)
                     /locus_tag="VHE33_16290"
     CDS             complement(6325..7266)
                     /locus_tag="VHE33_16290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HVT99066.1"
                     /translation="MHVHGANTAATQRVLRLSLIATVTYVVLTLLAGLRAHSLALISE
                     AGHNASDALAIVLSFVAVWFQTRPATDKKTFGYQRAGVLAAFLNALTLILISVWIALE
                     AIHRFSQPSPVQPRIMMFVAAAGVLMNGLIAAMLWRVSNDVNIRSVFIHMLGDTLSTA
                     AVIVGGLAILFTGATWIDPALSLAIAALILWSSATILRETLNILLEGTPRGISLGEIR
                     TILAAIDGVEDVHDLHVWSLGSNSHALSSHITIADIPPSESNLILDRINGVLRERFRI
                     YHTTLQFEHVVCETAHGCLVAVEDAAAHAHHHHGHVH"
     gene            complement(7309..8076)
                     /locus_tag="VHE33_16295"
     CDS             complement(7309..8076)
                     /locus_tag="VHE33_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99067.1"
                     /translation="MKDHLLFAEWLLCWHAANYAVMSDPVRGDQTHSEAATSAAANGR
                     AWFIQVALRSIAAGVAVIFAGHFDRRAIFTGAIVLTGSILIPVARRRFVPVRVLGEFE
                     LLANAGIAVLLWFMSHGLPGRNLPVETATGANRLAAICLCAALFVYAVRGGTYLVRGI
                     LEKAGGIPRPAIVSSEGYTHGRFIGQVERAIVFLIVIAGNLAALAFFFAAKGLIRSRE
                     LEERDRVDYLLLGSLSSFLVALAAGLVAQRILPFLWQ"
     gene            complement(8102..8800)
                     /locus_tag="VHE33_16300"
     CDS             complement(8102..8800)
                     /locus_tag="VHE33_16300"
                     /inference="COORDINATES: protein motif:HMM:NF027589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HVT99068.1"
                     /translation="MKSPQYVAVIGDMVGSSALNRSARAKTQISFTQLVDQLNADFQV
                     SLQGRFVIALGDGFEGLIHARAASAIVPILIWKIELTFTDLPIRLGFGFGGIDTALAP
                     NVSNLDGPTFHRAREAVEAAAKSGKMGGVFRGFGENHDAVLNGIARILHAHRSRWSRQ
                     QRNVAMLLHQGHRQSDVARIMSRSRQAVSTDARAANWAAYVEGEQGLTKALENAIAAR
                     ARWDTMPLRKTGST"
     gene            complement(8914..9402)
                     /locus_tag="VHE33_16305"
     CDS             complement(8914..9402)
                     /locus_tag="VHE33_16305"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HVT99069.1"
                     /translation="MFGQSLHDPRVIGTVAGAVLCIGAVTTWLILRRRPTAEEIERER
                     RALLVRSGRIIDGTILDISELENPTDQEHPREIRFILYKYEIGGVAYECSQEVTTLRD
                     LVNPTELRLGFPCSVRYDIHRPENSIVVAENWSGLRTTAESVPTRPMPQRPNPHASAP
                     VQ"
     gene            9540..10220
                     /gene="hslV"
                     /locus_tag="VHE33_16310"
     CDS             9540..10220
                     /gene="hslV"
                     /locus_tag="VHE33_16310"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: protein motif:HMM:NF003964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HVT99070.1"
                     /translation="MVQRRFSETAASGGRFSVSGKSTGRTAGPEEPGAPGRVRIRSTT
                     VICVRRGTSVVMAADGQVTMGEAVFKHSARKIRRLYQDKILAGFAGSTADAFSLFSRF
                     ENKLEQYAGNLGRAAVELAKDWRTDKMLRSLEALLVVSDLNQTFLIGGSGDVIEPDDG
                     ISAIGSGGSYALAAARALMENTELSARAIAEKAMKIAGQICIYTNDRITIEELNGPGA
                     GEKPVSGS"
     gene            10302..11876
                     /gene="hslU"
                     /locus_tag="VHE33_16315"
     CDS             10302..11876
                     /gene="hslU"
                     /locus_tag="VHE33_16315"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: protein motif:HMM:NF003544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HVT99071.1"
                     /translation="MAIYLPGTAEDQELSLEELTPREIVGELDKYVVGQKAAKRAVAI
                     ALRNRMRRQKLTPDLADEIMPKNIIMIGPTGVGKTEIARRLAKLTNSPFLKIEASKFT
                     EVGYVGRDVDSIVRDLVEIAIDMVREEKLEEVEDRAEMNAEERLLDVLLPPPAQPAPA
                     AGSPGAANATGFSGPTIEGNTITLPPPPGGAADSHNRTREKLRQQFREGKLDDRMVEI
                     DVRERGTPSFEIISNQGVEEMDINLKDMLPNIFGQRTRKRKMKVSDAFEYLVQEEEGR
                     LIDMDQVTRLAVERVESSGIVFLDEIDKIAGREGGHGPDVSREGVQRDILPIVEGTTV
                     NTRYGMVRTDFILFIAAGAFHVSKPSDLIPELQGRFPIRVELQSLTVEDFVKILTEPK
                     SSLVRQYTALLETEGLKLEFTPEAIEEMAQFAFKVNEGTENIGARRLHTIMEKVLDEI
                     SFQAPDLTRAQQKGDGQSTVVELAAVGEGTTAPLPVIERETANGTEKVMVIDPEYVRQ
                     MVASIVKNQDLSRYIL"
     gene            complement(11961..12659)
                     /locus_tag="VHE33_16320"
     CDS             complement(11961..12659)
                     /locus_tag="VHE33_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99072.1"
                     /translation="MTLRSISSYSTPMTDNRKAGWALILGSVGGIVTMAIHPVAGGPL
                     TPAQVQRLATVSGIAHGLALVSVLLLFLGTCGLTRALAAPDRLAFSALVTFGFAAVAI
                     MIAASVSGWIVPGIMKQMARDVLNNSASQSDYRIVIAAIFQINQAMARIYSVATAVAI
                     ALWSARCLRGGQLSRTMAWYGVVIAPLTAILILVGHLRLDVHGMAVVMLTEVIWFVGM
                     AIALRKPDAVITPA"
     gene            12755..13333
                     /locus_tag="VHE33_16325"
     CDS             12755..13333
                     /locus_tag="VHE33_16325"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT99073.1"
                     /translation="MAAPKSDARVLRSRDALGDAMIALMQEQPFERITVQQVLDRAGV
                     SRSTFYSHYRDKDDLFLSDVEDFFQMMGRLLTRYKAPARRLAPVEEFFAHLADVPGVQ
                     AAIDAAGKGPDVRELGIGCFARSIEERLTLAGVTLPAAELRATSHALAGSLFSLMDWW
                     LRSNKPITPRAADALFHRMAWSGLPSAEPVRS"
     gene            13351..14424
                     /locus_tag="VHE33_16330"
     CDS             13351..14424
                     /locus_tag="VHE33_16330"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99074.1"
                     /translation="MATTVRSFSKINLGLAIGPTRADGFHGLTTCYQTLAAHDLVTVE
                     AKAGSKTSLRLTSNDARVPTDDRNTAWKMVVLALGALKTEAEVHVHIEKRLPVQGGIG
                     AGSANAVAALIGLEQELAHAGVAELPGSERLRIAAEVGSDVPLFLIGGTVLGVGRGEQ
                     VAPLPSISPTECVLALPDVGVSTPAAYREWDEMQATGRRSQGAETAQQLTGNNGSDRL
                     ITLSRALASAFVPRLSGGLTAGVGSHSSGVSALSGDLAGKGNPLLALVRTGIENDFEE
                     VVFRQYPSLGHIKRILAGSGNPEEAAVYAALSGSGSALFGLYGTADAAAAAESRLKEA
                     GIRSLRTLTLARDEYWRTMVVHV"
     gene            14605..14678
                     /locus_tag="VHE33_16335"
     tRNA            14605..14678
                     /locus_tag="VHE33_16335"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14637..14639,aa:Gln,seq:ttg)
BASE COUNT         2959 a         4003 c         5144 g         2595 t
ORIGIN      
        1 agggcggtac cggagaagtc tgagcgggga aatattcctt gcaattcgta cgccgggagt
       61 atgggcttct tgctgtgggg tgaacctggc gttcgcgctc ctcggtggtc ggtgaatgtg
      121 gcgcgttcca aaaggggatt agggcggtac cggagaagtc taagcgggga aatattcctt
      181 gcatttcgca cgccgggagt atgggcttct tgctgtgggg tgaacctggc gtgcgcgctc
      241 gtcggggtgg ggtgtgtgcg cggagttcca aaatgggagc gggtcggtac cgaggaattc
      301 tgcgccgggc gatatccctt gcaattcgta cgccgggagt atgggcttct tgctgtgggg
      361 tgaacctggc cttacttaag gccgggcgcg cagttcgtga gtgggctggg gaccattggg
      421 gcactcagcg aagagtcagg ggcacgcccc tcagggcgcg aacaccgttt tatacttcgt
      481 cgagtctcgg tcccgaacag caggaaatta ttggtcgcgc cttgtggcta aacggcggct
      541 cgctcggcga ctcgcgggat ctcgaagcga taaccggctc cgcggaccgt tttcagatag
      601 cgaggattct cggggtcgga ttcgatcttt tcgcggatgc gacggacata aacgtcgacc
      661 gaacgcggcg taacgaagcg ggcgtctccc cagacagcgt ccaacaggtg gtcgcggctg
      721 aagacgcgac cgggatggcg ggcgagatag tcaaggaggc gaaattcggt ggcggtggtg
      781 gtggtgagac tgccgcgcac ccggagctgc atcgcgctgg catcgatttc gacgtcctca
      841 aaggcgatcg cggagggtgt ggacggacgc tcaaagcggc gaagaacagc tttcacgcgg
      901 gccagcagct cgcgcggggc gaagggcttg gtgatgtaat cgtcggcgcc aagctcgagg
      961 ccgaggacgc gatcgttttc cgcggcgcgc gcagtcacga agatgatggg gatggagctg
     1021 agggccggat gattgcggag tcgccggcag acatcgagcc cgtcgccgcc aggaaccata
     1081 atgtcgagca gaaagagggc gggcgcctgg cgctcggcat cgggaatgag actggcgggg
     1141 gtggcataga tgcgcgccgg aaagccggcg gcttcgagat gatgctggat caggcgggcg
     1201 atgtcggcgt catcctcaag cacgaaaacg gtctggctca agcgaagggt tcctttcccg
     1261 ggagtcccgg cagcgggtgg cgcattccgc cctcagccta aaccgggcga ggcaaagaga
     1321 ttgcaaatgt tgtgttaatt cggagcggag aaacaacagt caccgctcat ggcaatggcg
     1381 caaaggtggc aattctttca gcggtttaac cctgcaatca ggaggtttag gccggaaccc
     1441 cggatgttat acttcggtca accttttgtc tcgaatttca acgaccccgg aagactcagc
     1501 gcgatcatct ttcccctggg ggtcgggcag gcagagcagg ttctgtgcct attccaacca
     1561 tcctgtgcat tgacgacgaa gctctcgggc ttgaaatccg caaggcggtg ctggagcgcg
     1621 ccggatatca ggtgctgacg gccgtggatg gcgccacggg gctgaccttg tttcgcggaa
     1681 acccggtcga cggagtggtg ctggactatt acatgcccga aatgaatggc ggagccgtgg
     1741 cggaggcaat gcgccgcgag cggccggaaa tcccaatcat gctgctttcc gcctacatca
     1801 atttgcccac cgacgtggtt cagctggccg acgtgaccgt actgaagggc gaggggccgc
     1861 aggaactgct ggagaagctg cgagccatgc tgcattcggc agggatggca ggaggagcgc
     1921 gggcggaggg ctccgcgtga aggagctcca gttcaatcga acgctgcgcc agctggtggc
     1981 gctgccgatc atcctgctgg tgttcatggc gggattcctg gtatggcaga ttgccgcggc
     2041 atccattgca cagaaggcgc tcgatcacag cgatgaagtc acagcgcaac tgcaggagtt
     2101 gcagaacctg ttcgtcgatc aggagacggg gctgcgggga taccagctga ccggcgatcc
     2161 ggtgatgctg gagccgtgga aatcggctgc cggcccgatc cagaatcatt tcgcaacgct
     2221 gcggcggatg ctcgcaggag attcagagca gagcaagcgc ctcgaagacc ttcagatcct
     2281 ttacaaccag tggctgggat tcgccaacgc tgtgctgcac caggacgctg catcgctgaa
     2341 cgccgccggc atgaaccagc gcggcaaagc gatgatggac gggattcgat cggcgaccgc
     2401 ggacatggtg gggcacgagg cgaatttgcg tgcggtgcgg tcgcagcatg cacgctggat
     2461 gcagcggcgc gagctggttt ctctgctggt ttccgcggtg gtcgtgggac tctttctggc
     2521 tctgttcacg cgtcatcacg tgcatgccat ctccgcaagc tatcagcgga ggatcgcgga
     2581 gatcacaaag caatcgaacg aacttttcga gagccagcaa tggttcagga cgacgctgga
     2641 atcgatcgga gatgcagtga ttgcctgcga ttatcgcggg gctgtgtcgt tcatgaacga
     2701 cgtcgcggag aagctgaccg ggtggacctc gagcgaagcc aagggaaagc cgctggcgaa
     2761 ggtgtttcac atcgtcaacg agcagacgcg gaaggtctgc gagaacccgg tggacaaggt
     2821 gcgacggctg aaaaaagtcg ttggcctgtc gaatcacacg gtgctggtcg ggcgcggcgg
     2881 gcgcgaatac gtgatcgacg acagcgcggc gccgatcatg aacgacgccg cgaagatgat
     2941 cggtgtggtg ctggtgttcc gcgacgtgac ggaagaacgc caggtgcagg cggcgctgct
     3001 ggcgtcagaa aagctggcgg tagcgggtcg gctggcggcg agcattgcgc acgaaattca
     3061 taatccgctg gattcggtgg ccaacctgca ttatctgctg gctcaggaaa gcgatcccgc
     3121 gaaacgcgag gagtatctgc gtttggcgca gcaggagctg gggcgaacca tgcagatcag
     3181 ccggacgatg ttaagcctgt atcgcgagcc gcggtcgccc atccagatcg acctgcggga
     3241 actgattgaa ggcgtgctgc tgctgctgga tcggcgtctg cagaaccagg gcatcgtcct
     3301 ggagacgaaa attgccgggg atctttcagt ggagggcttc cctgccgagc tgcggcaggt
     3361 gttcaccaac ctgatcgtca acgcggtgga ggcggctggc gcgaaaggta aagtgcgcat
     3421 cacgatggag cgcgccgagt cggcagagat gcatggagct ggagccgcca tcgagatcgc
     3481 cgacagcggg cccgggattg cgccggaggc ggcgaagagg ctcttccagc cgttcttcac
     3541 gaccaagggc gcagacggca ccgggctggg gctgtgggtg agcatgggga tcgtgcaaaa
     3601 gcatggcgga tcgatcagcg tggtggagag ctccgatccg gaactgccgg gagcgtgcat
     3661 ccgggttttc ctgccggcgc gaaccatggc gcagcgggcc ggcgaaccgg ctgcttgagc
     3721 agagattcgt cagacagcgc gcagggcttc gaccggatcg gtgccggcgg cgcgattggc
     3781 cggcaacgcc gcggcaagaa tcgaaatcag gaacatggtt gcgatggtga cggtgtagat
     3841 cagcggatcg gtcgcgctga ctccgaagag aaacgcgact aacaggtgag aaaccgccag
     3901 cgatccgagg acaccgaggg tgcatccggc aaacgccagc ttcgctccgg acaggagcac
     3961 catgcgcgcg atgcttgcgc gctgcgcacc catcgccata cggatcgcaa tctcgcgcga
     4021 tcgcagcgtg actgaaaacg ccacgacggc gtagattccg gtcaaagcaa ggaggagcgc
     4081 ggcagcggca aagctggtca tgagatcggt gttgaagcgc cgcggcgctt cgacatttgc
     4141 catcgagtcg cgcattgttt ccacctgatc gagagccagc tgtgggtcga cttctgccac
     4201 ggaggatcgc agcacctgct tcatctggtc gggatcgagc gaggagcgga gagcgataaa
     4261 gcctccggcg gcctgaacga ggttctgctt cgcggcggag ccatagagga tcgagggctg
     4321 ctcggcgggc atgtaccact ggtccgcgac aggagcgtcg cggggaccga ggaccgtatt
     4381 gccgacgaca ccgacgacgg tcgcccaggg atatcccttt tgcggattac caacgtgaat
     4441 cgcttttccg agcgcgctct ggcctggcca gcagtgcttc gccatggcct cgttgacgat
     4501 gatgacgagc ggcgcgtcgg cgcgatcctg gggtgtgaaa tagcggccgg ccagcagagg
     4561 gattcccatg gtgcggaaat aatcaccgcc ggtgatgacg aatgcggcga aggtgagctt
     4621 ccaggtggcc gccggctgac cctcgacggt gtaggctgcc agccctccat tgttcgacgt
     4681 gggcagggag ttggtgaagc ctgctgcgac gacgcctggc ttcgccacga ggcggtcgat
     4741 gaccgcgtgg gtgaaattct caacctgggt ctgggtggag tactgctcga gcggcagctg
     4801 gtagctcgcg gcgagcacgt gttctggctg aaagccagga ttcacggcga gcatcttctg
     4861 gtagctgcgc aaaaaagagc ccgcagtggt gagcaggacg agagcggtgg cgatctcggc
     4921 gacgacgagg aaggatcgga gccagctgtg actgagggac cccgtgccgc tgcgtccggc
     4981 ctccttcagg ttgtcgatga cgctggcgcg cgtggcggcg aacgcaggag cggcgctgca
     5041 caatgctcct gtgaggaggg ctaaaagcag cgcgaagatc agaaccgccg ggtcgatggc
     5101 aatcgtgtcg acgcgcggca gagaatcggg aaggagacgc agcacggttc ggacggcgac
     5161 tgccgagagg ccgacagcca gagcgccgcc cgcgaggccg agtatgagac cggcgacgat
     5221 cgactgccgc aggattgcac gcggcggagc gcccagagcg gcctgcacgg cgtattcgcg
     5281 gcgtcgcccg atggctcgaa ccaggagcag cccggcgacg ttggcgcagg cgatggccag
     5341 cactatcagg accgcaacaa agagggcacg caggacggga cgggttgcgg cgacggactg
     5401 ctcgcgcagg gacaaggcat cgccccgaat gcggatggcg gccatggtcg ggggaaaatc
     5461 gcgcatgatt tgctgcgata cccgatgggc gtcctcggcg gcctgctgga gggtgacgcc
     5521 atctttcacg cgcgcgacca tgcgatacat ccaggcgccg acgtttttgt cggacagctc
     5581 ctcggcggtg aggctgagcg gaacccagac ctgtgccttg ttcatgcgac cggctgacag
     5641 cgggaactcg aagctgggcg gcatgacgcc gacgatcgaa tacgatttgc ggtcgagcac
     5701 gatcgcctgg ccgatgacat gcggatcgcg atgataccgg ttcagccaca gggcatcgct
     5761 gatgaccgcg acgggcgtgt ggccgtcgtc ttcctgcttc gtgaagacgc gccccagctc
     5821 gggggagacg ccgagtgcgg aaaagacgcc agcgcccagg cgtgctccgg cgatcaactg
     5881 ggccgtcgcg gcgccggaga tttcgtatcc cgcacccgca aagccgccca tcgaggaaaa
     5941 ggcggttgtg gcgcgctcgt aggtggcgat ctcgcgagcc gtgacgccgg gattggggtt
     6001 gttgccgagg tggtcgccga cgatgacgag gcgggcggga tcggcaaacg ggaggggccg
     6061 cagcagaatg ccttccacca gggagaagat cgcgatggtc gcgcccattc cgagagccag
     6121 cgtcaggatt gccgtcagat agaaacctgg cgccttgcgc atctgccgca gcgccaggcg
     6181 gacatcgcgc catgctccac tcatcgaatg ctcctctcgt gcatgcgcca ttacagcctg
     6241 cgcgtgggag gacgggcaaa tgctttcgtc acaaaaacgc tggtggtcgt caacggggag
     6301 ggcgcgaccg ggcctttctg cgggtcagtg aacgtggccg tggtggtgat gcgcgtgagc
     6361 ggctgcgtcc tcaacggcga ccaggcagcc gtgcgccgtc tcgcaaacca catgctcgaa
     6421 ctgcagcgtg gtgtggtaga tgcggaagcg ttcgcgcagc acaccgttga tgcggtcgag
     6481 aatgaggttg ctctccgacg gggggatgtc ggcgatggtg atgtggctgg agagcgcgtg
     6541 gctgttggag cccaggctcc agacatgcag gtcgtggaca tcttcgacgc cgtcgatagc
     6601 ggcgaggatg gtgcgaattt cgccgagcga gatgccgcgc ggggttcctt cgaggaggat
     6661 gttgagagtc tcgcgcagga tggtcgccga tgaccagagg atgagagcgg cgatggcgag
     6721 cgagagagcg gggtcgatcc aggtagcgcc cgtgaacaga attgcaaggc cgccaacgat
     6781 gacggctgcg gtggagagcg tgtcgccgag catatggatg aacacgctgc ggatattgac
     6841 gtcgttgctg acacgccaga gcatcgcggc aatgaggccg ttcatcagaa cgccggcggc
     6901 agcgacgaac atcatgatgc ggggctggac gggggagggc tggctaaagc gatggatggc
     6961 ttcgagggcg atccagacgg agatgaggat cagggtgaga gcgttgagga aggcagcgag
     7021 gaccccggcg cgctggtagc cgaaggtctt tttgtcggtg gcgggacggg tctgaaacca
     7081 gactgcgacg aaggagagga cgatggcgag agcgtcggag gcgttatgcc cggcttcgga
     7141 gatgagggcc agggaatgcg cgcgcagtcc ggccagcagc gtgagcacaa cgtaggtaac
     7201 ggtggcgatg agagagagcc gcagcacccg ctgcgtggcg gcggtattcg cgccatggac
     7261 atgcatattg gttccagttt atcgcggggg cttcagacgg ccgcgccgtt attgccagag
     7321 aaaaggaagt atcctttgtg cgacaagtcc cgcggcgagg gcgacaagaa aactgctcag
     7381 agagcccaga agcagatagt cgacgcgatc acgctcttcc agttcccgcg atcgaatcag
     7441 ccccttggct gcgaaaaaga acgcaagagc ggccagattg ccggcgatca cgatgaggaa
     7501 gacgatggcc ctttccacct ggccgataaa tcttccgtgc gtgtatcctt cgctggatac
     7561 gatggcggga cgcgggattc cgcctgcttt ttccagaatg ccacgcacca gataggttcc
     7621 cccacgcact gcataaacga agagggccgc gcacagacaa atcgccgcca ggcgattggc
     7681 tccggttgcg gtctccaccg gaagatttct tcccggaagg ccgtggctca tgaaccacag
     7741 aagcacggcg attcccgcgt ttgctaatag ttcgaattcc ccgagcactc gaacgggtac
     7801 aaagcgcctg cgcgcgacgg gtatcagaat cgatcccgtg aggacgatcg cccccgtgaa
     7861 aatcgctcgg cgatcgaagt gaccagcgaa aattaccgca accccggctg caatcgaacg
     7921 cagagcgacc tgaatgaacc atgcgcgccc gttggccgcg gcgcttgttg cggcttctga
     7981 gtgcgtctga tcgccgcgca ccgggtctga catcaccgcg tagttggccg catgccagca
     8041 gagaagccac tcggcaaaga ggagatgatc cttcatagcg cttgtgagct ggaacggcgg
     8101 atcaggtcga tcctgttttc ctgaggggca tcgtatccca tcgggcgcga gccgcaattg
     8161 cgttttcgag ggctttcgtg agcccctgtt cgccttcaac ataggctgcc cagttcgccg
     8221 cgcgcgcgtc cgtcgaaacc gcctgtctcg atcgggacat gattctggca acgtcgcttt
     8281 gtcgatgtcc ctggtggagc agcatcgcga cattgcgctg ttgccggctc caccggctgc
     8341 ggtgcgcgtg cagaattcgc gcgatcccgt tgagaacggc atcatgattc tcgccaaacc
     8401 cccggaacac cccgcccatc tttccggatt tggccgctgc ctctacagcc tcgcgcgcac
     8461 gatgaaatgt cgggccatcc agattcgata cgttcggcgc gagggctgta tcaatgccgc
     8521 cgaatccaaa tccgaggcgg atgggaaggt cggtaaaggt aagttcgatc ttccagatca
     8581 gaatgggaac aattgcactt gctgcccggg cgtgtatgag cccttcaaag ccatctccca
     8641 acgcaatcac gaaccttccc tggagagaaa cctggaaatc cgcattcagc tgatcgacaa
     8701 gttgcgtgaa cgagatttgg gttttggcgc gggccgaacg gttcagcgca ctggacccca
     8761 ccatatcccc gataaccgcg acgtattggg gggatttcat ttgtaaatat tagaatattg
     8821 actcaagcag aatcaataaa taattgttga ctaattggaa gtccacggaa ttaccttgac
     8881 attgccgatg tcaataatat tccatttaca cttttactgc accggcgcag aagcgtgcgg
     8941 attggggcgc tggggcatgg gccgggtggg aaccgattcg gccgtggtgc ggaggccgct
     9001 ccagttttcc gcgacgacga tgctgttttc ggggcggtga atgtcgtagc ggaccgagca
     9061 ggggaaacca aggcggagtt ctgtggggtt cacgaggtcg cgcagagtgg tgacttcctg
     9121 ggagcactcg taggcgacgc cgccgatttc gtacttatag aggatgaagc gaatctcgcg
     9181 cgggtgttcc tggtcagtgg ggttctcgag ttcggagatg tcgaggatgg tgccgtcgat
     9241 gatacggccg gaacggacca aaagtgcgcg gcgctcgcgc tcgatctctt ccgcggtggg
     9301 acggcggcgc aggatgagcc aggtggtaac cgcgccgatg catagaaccg cgcctgcaac
     9361 cgtcccaatg acgcggggat cgtgcagtga ttggccgaac atccggtgga ctccgaaggg
     9421 acggggactg aaagcaccga cctttgcaga atagctcagt tgtgcgaatt caccgaggga
     9481 aaagctggtg ctccgatgca aagaagtaac ggtacggctg cgctatactc gcagaagaca
     9541 tggtgcaacg cagattttca gaaacggccg cttcgggcgg tcgtttttcc gtcagtggaa
     9601 aaagtactgg ccgtacggcc ggaccagagg agcccggtgc accgggcagg gttcggatcc
     9661 ggtcgacaac cgtcatttgc gtccggcgcg ggacaagcgt ggtgatggcc gccgacggcc
     9721 aggtcacgat gggcgaagct gttttcaagc acagcgcacg gaagatccgg cggctctacc
     9781 aggacaagat tctggcgggg tttgcgggat cgacggcgga tgcattctcc cttttcagcc
     9841 gcttcgaaaa caagctggag cagtacgcag ggaatctggg gcgtgcggcg gtggagctgg
     9901 cgaaggactg gcgcacggac aagatgctgc ggagtctcga ggccttgctg gtggtatcgg
     9961 acctgaacca gacgtttttg atcggcgggt cgggcgacgt gatcgagccg gacgacggga
    10021 tttcggcgat cggcagcggc ggatcgtatg cgctggcggc ggcacgggcg ctgatggaga
    10081 acaccgagct aagcgcccgc gcgattgccg agaaggcgat gaagatcgcg ggacaaatct
    10141 gcatttacac caacgaccgg atcacgatcg aagagctgaa cgggccgggg gctggggaaa
    10201 agccggtcag cggcagctaa gaggcggccg gctcagggcc ggtcagcgat gaagcggtca
    10261 gcgatgggac ggtcagcaaa aagctgggaa gcgggattga tatggcgatt tatttacctg
    10321 ggacggcaga ggatcaggag ctttcgctcg aagaactgac gccgcgggag atcgttggcg
    10381 agctggacaa gtatgtcgtg ggccagaagg cggccaagcg ggcggtggcg atcgcgctgc
    10441 gcaatcgcat gcggcggcaa aagctgacgc cggatttggc cgacgagatc atgcccaaga
    10501 acatcatcat gatcgggccg acgggcgtgg gcaaaacaga gatcgcgcgg cggctggcaa
    10561 agctgacgaa ctcgcccttc ctgaaaatcg aggcgtcaaa gttcaccgaa gttgggtatg
    10621 tggggcgcga cgtggattcg atcgtgcgcg atctggtgga gatcgccatc gacatggtgc
    10681 gcgaggagaa gctcgaagag gtcgaggatc gcgcagagat gaacgcggag gagcggctcc
    10741 tggatgtgct gctgcctcct ccggcgcaac ctgcgcctgc ggctggctcg cctggagccg
    10801 ccaatgcgac tgggttttcc ggcccgacga tcgagggcaa cacgatcacc ttgccccctc
    10861 cgcccggtgg cgctgcggac tcgcacaatc ggacgcggga gaagctacgg caacagttcc
    10921 gcgaaggtaa gctcgacgac cgcatggtgg agatcgacgt gcgcgagcgg ggcacgccgt
    10981 ccttcgagat catctccaac cagggcgttg aggagatgga catcaacctg aaggacatgc
    11041 tgccgaacat ctttgggcag cgcacgcgca agcggaagat gaaggtgtcg gacgcgttcg
    11101 agtacctggt ccaggaagaa gaaggccggc tgatcgacat ggatcaggtg acgcggctgg
    11161 cggtggaacg ggtggagagc tcgggcattg tctttctcga cgagatcgac aagattgccg
    11221 ggcgcgaggg cgggcacggg ccggacgttt cgcgcgaggg cgtgcagagg gacattctgc
    11281 cgatcgtgga aggcacgacg gtgaacacgc gctacggcat ggtgcggacg gacttcatcc
    11341 tgttcatcgc ggcgggcgct ttccatgtgt cgaagcccag cgacctgatt ccggagctgc
    11401 aggggcgttt cccgattcgc gtggagctgc agtcgctgac ggtggaggac ttcgtcaaga
    11461 ttctgactga gcccaagtcg tcgctggtgc ggcaatacac ggctctgctt gaaaccgagg
    11521 ggctgaagct ggagttcacg ccggaagcga tcgaggagat ggcgcagttc gcgttcaagg
    11581 tgaatgaggg cacggaaaac atcggagcgc ggcggctgca cacgatcatg gagaaggtgc
    11641 tggacgagat cagcttccag gcgccggacc tgacgcgcgc ccagcagaag ggcgacggcc
    11701 agagcactgt cgtcgagctg gcggccgttg gtgaaggaac gacagcaccg ctgccggtca
    11761 tcgagcgcga gacggcgaac gggacggaga aggtgatggt catcgacccg gagtatgtgc
    11821 gacagatggt ggcgagcatc gtgaagaacc aggatttgtc gcgatatatc ctctgacggc
    11881 agctaaagtc cggtcagcta agaagcggtc agctatggtc cggttagcga agaagcggtt
    11941 ggcgaaaggc cgctttgcgg tcaggccggc gtgatgaccg cgtctggctt ccgtaaagcg
    12001 atggccattc ccacgaacca gatcacttcg gtcagcatga ccacggccat gccgtgcacg
    12061 tcgaggcgga gatgtccgac gaggatcagg attgcggtca gcggagcgat gacgacgcca
    12121 taccaggcca tcgtgcgcga gagctggccg ccacgcaggc agcgggcaga ccagagggcg
    12181 atggccaccg cagtggcgac agagtagatg cgcgccatgg cctggttgat ctggaagatc
    12241 gccgcgatca cgatccggta gtcggactgg ctggcgctgt tgttgagaac gtcgcgcgcc
    12301 atctgcttca tgatgccggg aacgatccag ccgctgacgg aggcggcgat catgatggcg
    12361 accgcggcaa acccgaaggt caccagtgcc gagaacgcca ggcgatcggg ggcggcgagc
    12421 gctcgggtga gcccgcaggt tccgagaaac agcagcagca cgctgacgag agcgaggccg
    12481 tgtgcaatgc cggagacggt cgccaaacgt tgaacctgag ccggggtcag cgggcctccg
    12541 gcaacgggat ggatggccat cgtgacgatt ccgccaacgg aacccagaat cagcgcccag
    12601 cctgccttgc gattgtcggt catgggcgta ctgtaggagg aaatcgatcg caaagtcatc
    12661 ccccaccgcg cgataacgaa caagggaggg ccctttgtcc ggaaaagctt caggaaacgc
    12721 cgcgagtgag actgcgcaaa tcgctcgatc cgaattggct gcacccaaat ccgacgcgcg
    12781 cgtgcttcga tcccgcgacg cgctgggcga cgcgatgatc gcactcatgc aggagcagcc
    12841 tttcgagcgc attacggtgc agcaggttct ggaccgtgcg ggtgtcagcc gcagcacctt
    12901 ctattcccac taccgcgaca aggacgatct gttcctttcc gacgtggaag atttctttca
    12961 aatgatgggc cggctgctga cgcggtacaa agcgccggcc aggcgtctgg ctccggtgga
    13021 ggaattcttc gcccatcttg cggatgttcc gggagtgcag gcggcaatcg atgcagcggg
    13081 caaaggtccg gatgtccgcg aactcgggat tggttgtttt gcgcgatcga tcgaggagcg
    13141 attgaccctg gccggcgtta cgttgcctgc cgctgagctg cgggcgactt cccatgcact
    13201 tgccggctcg ttgttctcgc tgatggactg gtggctccgc agcaacaagc ccatcacgcc
    13261 gagggccgcc gatgcgctgt tccatcgcat ggcgtggagc ggtttgccat ccgccgagcc
    13321 tgtgcggtcg tgagtcttag aattggaacg atggccacga cggttcgttc gttttcaaag
    13381 atcaatctcg ggctggcgat tgggccgacg cgcgcggatg gttttcatgg tctgacgact
    13441 tgctaccaga ccctcgcggc gcatgatctg gtgaccgtgg aggcgaaggc tgggtcgaaa
    13501 acgagccttc ggctcaccag caacgatgcc cgcgttccga cggatgatcg caacaccgcc
    13561 tggaagatgg tggtgctggc actcggcgca ctgaagacgg aggcagaagt tcacgtccac
    13621 attgagaagc ggctgccggt gcagggtggg atcggggcgg ggtcggccaa tgcggtggcg
    13681 gcgctgattg gcctggagca ggagctggcg catgccggcg tggcggagct gccggggtcg
    13741 gagcggctgc ggatcgcggc agaagtggga tcggatgtgc cgctgtttct gatcggggga
    13801 acggtgctgg gcgttgggcg cggcgagcag gtcgcacccc tgccaagtat ttcgccgacc
    13861 gagtgcgtgc tggcgctgcc cgatgtcggc gtgtcgacgc cggcggcgta ccgggagtgg
    13921 gatgaaatgc aggccacagg gcgccgatcg cagggcgcag aaacggcgca gcaattgacc
    13981 ggaaataacg gctccgatag actgataacg ttgagtcgag ctctcgcctc ggcctttgtg
    14041 ccgaggctct ccggagggtt aaccgccggg gttgggtcgc actcctccgg tgtctccgct
    14101 ttgagcgggg accttgcagg gaaggggaat ccgcttctcg cgcttgtccg aaccgggatc
    14161 gaaaacgact tcgaagaggt cgtcttccgg caatatccct ctctcggaca tatcaagcgc
    14221 attctggcgg gctccggcaa tcccgaggag gcggccgttt atgcggcgct ctccggctcc
    14281 ggatcggccc tgttcgggct ttacggaacg gcggacgcgg cggcagcggc ggagtctcgg
    14341 ctaaaggaag cgggtattcg atcgctgcga acgttgacgt tggcgcggga cgaatactgg
    14401 cggacgatgg tggtgcacgt ttagcgaaaa gccggtcggc taagaaccgg ttagctaaaa
    14461 agcggttagc aggggacgtt tagcaaggaa cttttagcga aagacgttca gcggcaggga
    14521 gacggcagat ttcaccaaca gcaattcgct aaacgcattt ggctaacagc atttggctaa
    14581 gtgcattttg ctgaacacga atactgggcg atcgactaat ggtaggtcaa gtgcctttgg
    14641 agcactttgt ccaggttcga atcctggtcg cccagccaaa attttgtaac ggcccggcag
    14701 a
//
LOCUS       DASZYN010000637         7545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1252500, whole genome shotgun sequence.
ACCESSION   DASZYN010000637 DASZYN010000000
VERSION     DASZYN010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7545
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252500"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VHE33_16340"
     CDS             <1..339
                     /locus_tag="VHE33_16340"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVT99075.1"
                     /translation="IQRDIFRFVGKHAWVVKRNMDSLLTGNDLLDGKLGALKMPMLIV
                     WGKEDHLIPVAVGEAMHKAIPQSEFVVVDGCGHLAPGRCAAQIGPVFRGFLDERSPIP
                     GQRAEILRKR"
     gene            complement(336..572)
                     /locus_tag="VHE33_16345"
     CDS             complement(336..572)
                     /locus_tag="VHE33_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99076.1"
                     /translation="MHCDSLAPLTVLATLAAFIISICFRQMGRFPDKRTRWILLVFLL
                     IAAGALFADFRYVRRYRAFCDELQQQVQPIQPAH"
     gene            complement(701..1360)
                     /locus_tag="VHE33_16350"
     CDS             complement(701..1360)
                     /locus_tag="VHE33_16350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HVT99077.1"
                     /translation="MSSAHGAEERARRIKLIIFDVDGVLTDGGIWLFPAPAPSTTTAD
                     HAAQMESKGGYAISSQSMVEAKGFNAHDGTGISLAKLAGLKCAIITKRISETVALRAR
                     DLRLEYVYMGQSYKMKAVREMMEKEQIGLEEIAYVGDDVIDLPVMRECGFAVAVANAR
                     DNVKVEAHWVTPHEGGHGAARDAIEFILAAKGILEKTIESYIDERNPVPASMDIGKGG
                     F"
     gene            1498..3474
                     /locus_tag="VHE33_16355"
     CDS             1498..3474
                     /locus_tag="VHE33_16355"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT99078.1"
                     /translation="MMRLWRLLYYARRYMLQALSAVILAAGVGLLDAFRVLLIGPIFD
                     NVLHPGAASRANKTQLLGSIGERWHISITPQILVPSFLHIKNDWAVIAFAFVAATLLK
                     GICDYAGTYLANYAGFGMITDMRDDLYESILRRSVSFFQKHTTGTLLSALINDIERVQ
                     YATATILSDFLQQACTLACMVVIVIALGGRLAWVLPAFGAIAAWSIFRIGRDVRRTTR
                     RGQDKLADIQNILHETITGNRIVKAFNMELWELLRFRQAAKRLLRANLRSVRAQAITS
                     PLMDFIGAVAIAALLWVGRNEIGHGAMAEGTFFAFIVALFKMYDPIRRFGTYYNNFQQ
                     AVGASAAIFDFMDDQDEVREAKHPTVLKGFKDSIALENVSFHYREEDREGPPVVLSDI
                     NLTINRGEVVAVVGPSGAGKSTLVNLIPRFFDVTDGRILVDGIDVRQASLASLRAQIG
                     KVTQETILFNDTVRNNIAYGQPGVPFNRVEDAAKAALAHDFIERMPDGYDTVIGEKGF
                     RLSGGERQRLAIARAILKNAPILILDEATSALDAESESLVQAALANLMTGRTVIVIAH
                     RLSTVRRANRIVVLEHGQITAIGSHEELLHTSPIYQKLYRLQFMDIGESNGNGNGNHI
                     GGNEPEAADPGATVESAIPVAKAAEPAFTGKIKR"
     gene            3471..4445
                     /locus_tag="VHE33_16360"
     CDS             3471..4445
                     /locus_tag="VHE33_16360"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HVT99079.1"
                     /translation="MSPVKPPTKTDKPAAPSAKVSVESEKQAVYSMTGFARVAGRVSE
                     TLGFSLSLKSVNHRFLDLHMRLPNGAEGVEMNLRRVLKEKLRRGHVEVTLSLERSQKA
                     EAGYDHALVAAYIEAFRAAATEHRLTAEPDLNSVFRLPGVLLAESRSSEEEMKALEEA
                     VRHETDGLIAALQAMRAQEGAVLANELRSGMKRLRVLVDEAATLREKVQQAYFERLNQ
                     RMKAMLDGGFDKDRILQEAALIAERSDVEEEVTRLRTHIDHFIGLLDQGGEVGKKLDF
                     LLQEMNREANTLLSKTSGVAGNGTRITEAGLGMKSEIEKAREQVQNLE"
     gene            4469..5194
                     /gene="gmk"
                     /locus_tag="VHE33_16365"
     CDS             4469..5194
                     /gene="gmk"
                     /locus_tag="VHE33_16365"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HVT99080.1"
                     /translation="MSGILFIISAPSGSGKSTLVNQLRSLVSGLEFSVSWTTRGPRGS
                     EVDSREYHFTTREHFEEMIRNGEFLEHAQVFGNYYGTAKSTLKEAFAQGKDLLLDIDV
                     QGAAQVRTKCPDAVSIFLMPPSPEVLATRLRNRSRAEGAVQEEIIERRLGRALVEIEN
                     YREYGYILVNDVLDRAVEEMVAIVAAERVRRSGDTSLSATDSNRVLALAESCRQANSQ
                     SRIRTVLRAFGVQEPGTNGQGHA"
     gene            5213..5446
                     /gene="rpoZ"
                     /locus_tag="VHE33_16370"
     CDS             5213..5446
                     /gene="rpoZ"
                     /locus_tag="VHE33_16370"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HVT99081.1"
                     /translation="MSIEKEYDSNFRYVLVAARRARQLQNGAEPMVPSRSRKACRVAQ
                     EEIAAGKITYVKAQDAPTLRPEVDGADIPKFAS"
     gene            5522..6781
                     /gene="coaBC"
                     /locus_tag="VHE33_16375"
     CDS             5522..6781
                     /gene="coaBC"
                     /locus_tag="VHE33_16375"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HVT99082.1"
                     /translation="MKVTVGVTGGIAAYKSAELVRDLQKHALDVHVVMTAAAQQFIAP
                     LTFAALTGHKVITGLWAPVGSDAGDATSAIEHIDAAQSTDALVVAPATADILAKFAHG
                     IADDFLTTMYLATTAPVIVAPAMNVNMWNHPATQANLEVLASRHVRIVAPESGYLACG
                     MVGSGRLAENSAIVDAVLSVLLHRNDLAGETVLITAGGTREALDPVRFLGNRSSGKMG
                     YAMAEAAQRRGARVILVSAPTALRPPANVEVVPVVTTQQMRDAVLGRLDQATIVIKAA
                     AVADYRPRAQADQKLRRKGPLTLELEPTEDILAAVVAQRRPGMLVVGFAAETEDVLSH
                     GRDKLLRKGADAIVLNDVSREGLGFDSDRNAVTFLTLDRAIELPELTKRELADQILDE
                     ILTLRSPRKEVLSESDSDLPLSLGEQA"
     gene            6976..>7545
                     /locus_tag="VHE33_16380"
     CDS             6976..>7545
                     /locus_tag="VHE33_16380"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HVT99083.1"
                     /translation="MSAVFRSDEDLETAEEMPAARVRPQPLSVLTQSTPPVAPEDRAA
                     ALQAIREDIGNCTRCALHKGRHTIVFGDGDPNARLMFVGEGPGADEDAQGLPFVGRAG
                     QLLNNMIGAMGLRRDQVYIANIVKCRPPQNRVPEPVEANTCAPFLFRQIDAIRPEVIV
                     ALGATAATYLLGGKSPLAALRGRIHHARDS"
BASE COUNT         1444 a         2392 c         2250 g         1459 t
ORIGIN      
        1 atccagcgtg acatcttccg tttcgtgggc aagcatgcgt gggtggtgaa gcgaaatatg
       61 gattcgctgc tgaccggcaa cgaccttctc gacggcaaat taggagccct taagatgccg
      121 atgctgatcg tttggggaaa agaggatcac ctcatccctg tggcggttgg tgaggccatg
      181 cacaaagcaa tcccgcagtc ggaatttgtg gtcgtcgacg gctgcggcca cctggccccg
      241 gggcgctgcg cggcgcagat tggcccggtg ttccggggtt ttctggatga gcgttccccg
      301 atcccaggcc agcgggcgga gatcctcagg aagcgttagt gcgccggttg tatcggctgc
      361 acctgctgct gtaattcatc gcagaacgcc cggtacctgc gcacgtagcg aaaatcggcg
      421 aacagcgccc cggcggcgat cagcagaaag accaggagaa tccatcgggt gcgcttgtcg
      481 ggaaaccgcc ccatttgtcg gaagcagatt gaaataatga acgccgccag cgtcgccagc
      541 accgtgagcg gtgcaagcga atcgcaatgc accgccgtct cctgaataaa ccatgccgag
      601 gaaaccgccg tgatgatcgc gattccgccc gtaatgtaga tgcgcgaccg cttcggcggc
      661 tgtctctgcg ccctgagctc tgcgccctgc gccctgttct tcagaatcca cccttcccga
      721 tatccatgct ggccggcacc gggttccgct cgtcgatgta ggactcgatc gtcttctcga
      781 ggattccttt ggcggccaga atgaattcga tggcatcgcg cgcggcgcca tgtcccccct
      841 catgcggcgt cacccagtgt gcctcgactt tcacgttgtc gcgcgcattc gccaccgcca
      901 ccgcaaagcc gcattcgcgc atcaccggca gatcgatcac gtcgtcgcct acgtaggcga
      961 tctcctcgag gccgatctgc tccttttcca tcatttcgcg cacggccttc atcttgtacg
     1021 actggcccat gtagacgtac tcaaggcgca ggtcacgcgc gcgcagcgcc acggtttcgg
     1081 agatgcgctt ggtgatgatg gcgcacttca atcccgcgag cttggccaga gagatgcctg
     1141 tcccatcgtg cgcgttgaaa cctttcgcct cgaccatact ctgcgaggag atcgcgtacc
     1201 cgcccttgct ctccatctgg gcggcgtgat ccgccgtcgt tgtactcggt gccggggccg
     1261 ggaagagcca gatgccgccg tcggtcagca ccccatccac gtcgaagatg atcagcttga
     1321 tgcggcgcgc tcgttcttcg gcgccgtgcg cagaggacat cgtcataccc gcattgtaac
     1381 gagtgcctgc caatgtaccg gatccgcgca cacggtgagc tatatcaccg gttcagcacc
     1441 agttgcggga taaaatcaag ggatgcgttg tttcccgctg ccgggagtat gccgggcatg
     1501 atgcgccttt ggcgtttgct gtactacgct cgccgctaca tgcttcaggc tctttccgct
     1561 gtgattttgg cagctggcgt cggcctgctc gacgcattcc gcgttctgct catcggaccg
     1621 atcttcgaca acgtcctcca tccaggcgct gccagccgcg cgaacaaaac ccagctgttg
     1681 ggttccatcg gcgagcgctg gcacatcagc attacgccgc aaatcctggt gccctcgttt
     1741 ctgcacatca aaaacgactg ggctgtgatc gccttcgcgt ttgttgctgc gactctgctg
     1801 aagggtatct gcgactacgc cggcacctac ctggcgaact acgccggctt cggcatgatc
     1861 accgatatgc gcgatgacct ttacgagtca atcctgcgcc gctcggtttc cttctttcag
     1921 aagcacacga ccggcactct gctgtcggcg ctgatcaacg acattgagcg cgttcagtac
     1981 gcgaccgcga ccatcctgtc ggactttctg cagcaggcct gcactctcgc ctgcatggtg
     2041 gtcatcgtca ttgccctcgg cggccgtctg gcctgggtgc tgccggcctt tggcgccatc
     2101 gctgcctggt cgatcttccg catcgggcgt gacgtgcgcc gcactacgcg tcgcggccag
     2161 gacaaactcg ccgacatcca gaacatcctg cacgagacca tcaccggcaa tcgcatcgtg
     2221 aaggccttca acatggagct ctgggaactg ctgcgtttcc gccaggcggc gaagcggctg
     2281 ctccgggcta atctgcggtc ggtgcgtgcc caggccatca cctcgccttt gatggatttc
     2341 attggagccg tcgcgattgc ggccctgctt tgggtcgggc gcaacgaaat cggccacggc
     2401 gcgatggcgg aaggcacctt cttcgctttc atcgtggccc tgttcaagat gtacgacccg
     2461 atccgccgtt ttggcactta ctacaacaat ttccagcagg ccgtgggcgc ctcggccgca
     2521 atcttcgact tcatggacga ccaggacgaa gtgcgggaag cgaaacatcc caccgtcctc
     2581 aagggcttca aagacagcat cgcgctggaa aatgtcagct tccactaccg ggaagaggat
     2641 cgcgaaggtc ccccagtggt cctgagtgac atcaacctca ccatcaatcg gggtgaagtg
     2701 gtcgcagtgg ttgggccgag cggagcaggg aagtcaacgc tggtgaatct cattccgcgt
     2761 ttcttcgatg tcacagatgg acgcatcctc gtcgatggca tcgacgtgcg ccaggccagt
     2821 cttgcttcgc tgcgcgcgca gatcggcaag gtcacccagg aaacgatcct cttcaacgat
     2881 acggtgcgca acaacatcgc ctatggccag cccggtgtcc ctttcaaccg tgttgaggac
     2941 gcggccaaag cggcgctcgc ccacgacttc atcgagcgca tgcccgacgg ttacgacacg
     3001 gtgattggcg agaagggttt ccgcctctcc ggcggtgagc gtcagcgtct tgctatcgcg
     3061 cgcgccattt tgaaaaatgc gcccatcctg attctcgacg aagccacttc cgcacttgat
     3121 gccgagagcg aatcgctggt gcaggctgcg ctggccaatc tcatgaccgg ccgcactgtc
     3181 atcgtgatcg cgcatcgtct ttcaacggtc aggcgtgcca accgcatcgt cgtgctcgag
     3241 catgggcaga tcaccgcaat cggttcccac gaggagctcc tgcacacctc gcccatttac
     3301 cagaagctct atcgcctcca gttcatggac atcggcgaaa gcaatgggaa tgggaacggg
     3361 aatcatattg gcggcaacga gcccgaggcc gcagatccag gagccaccgt cgaatctgcg
     3421 attcccgtag ccaaagccgc ggaaccggcg ttcacaggta agatcaagcg atgagcccgg
     3481 tgaagcctcc cacaaaaacc gacaagccgg ccgcgccatc ggcgaaggta tcggtggagt
     3541 ccgagaagca agcggtgtat tcgatgactg gcttcgcgcg cgttgcgggc cgcgtttccg
     3601 agacgctggg tttctctctc agtctgaaaa gcgtgaacca tcgctttctc gacctgcaca
     3661 tgcgtctgcc caacggggcc gaaggcgtcg agatgaacct gcggcgggtt ctcaaggaga
     3721 aactgcgccg cggccacgtt gaagtcacgc tgagtcttga gcgcagccag aaggccgaag
     3781 ctggttacga tcacgcgctc gtggccgcgt acatcgaggc attccgcgcc gctgccaccg
     3841 agcacaggtt aaccgccgaa ccggatctga attccgtctt ccgtcttccc ggggttctgc
     3901 ttgcggagtc gcgcagcagt gaagaagaga tgaaagcgct ggaggaagcc gttcggcacg
     3961 agacggacgg tctgattgcg gcgctccagg cgatgcgcgc ccaggaaggc gccgtgctcg
     4021 cgaatgagct gcgctccggg atgaagaggc tgcgggtgct ggtagatgaa gcggccaccc
     4081 tccgcgagaa ggtccagcag gcctattttg aacgcctcaa ccagcgcatg aaggcgatgc
     4141 ttgacggtgg cttcgacaag gaccgcatcc tccaggaagc cgcactgatc gctgagcgca
     4201 gcgatgtaga agaagaagtg acccggctgc gcacccacat cgaccatttc atcggtctgc
     4261 tcgatcaggg cggcgaagtc ggcaagaagc tcgattttct gctgcaggag atgaaccgag
     4321 aggcgaatac gctgttgtcg aagaccagtg gtgtcgccgg caacggaacg cgcatcaccg
     4381 aagccggcct gggaatgaag tcggaaattg aaaaagcgcg cgagcaggtg cagaacctgg
     4441 agtagggaag cgaggcgaac acaaaagcgt gtcgggaatc ctgttcatca tttcggcgcc
     4501 gtcggggtcg ggcaagtcga cgctcgtgaa tcagctgcgt tcgctggtca gtggactcga
     4561 attctcggtt tcctggacga cgcgggggcc gcgcggctcc gaagtcgatt cgcgcgagta
     4621 tcactttacg acccgggagc acttcgagga gatgatccgc aacggcgaat ttctcgagca
     4681 tgcgcaggtc ttcgggaatt attacggcac agcaaaatcc acgctgaagg aagcgttcgc
     4741 tcagggcaag gatctgctgc ttgatatcga cgtccagggc gcggcgcagg tccgtacaaa
     4801 atgtccggac gcggtgtcga tctttctgat gccgccctcg ccggaagtgc tcgccacacg
     4861 gctgcgcaac cgctcccgag ccgaaggcgc tgttcaggaa gagatcatcg agcgccgact
     4921 cggccgtgcc ctcgttgaga ttgagaatta ccgcgaatat ggctatattc tcgtcaacga
     4981 cgtcctggat cgagccgttg aggagatggt tgccatcgtc gctgctgagc gcgtccggcg
     5041 aagcggggat accagtctct ctgcaacgga ctcgaatcgc gtgcttgcat tggccgaaag
     5101 ttgccgccag gcaaattctc aatcgcgcat caggactgta ttacgcgcct tcggcgtaca
     5161 ggagcccggc acaaacggcc agggccatgc ttagagttct ggaggtagga cgatgagtat
     5221 cgagaaagaa tacgacagca atttccgcta cgtgctggtg gccgcccgtc gcgcacgcca
     5281 gctccagaac ggtgccgaac cgatggtgcc cagccggtcg cgcaaggcct gccgtgtcgc
     5341 ccaggaagag attgctgccg gcaagatcac ctacgttaag gctcaggacg cacccacgct
     5401 gcgtcccgaa gtcgacggtg ccgacattcc gaaatttgct tcgtagctgc aacctgtaca
     5461 ggcggagagt gggcccggtt atcctctgta ccccgcccct tctaccctgt accctgtact
     5521 catgaaggtc acggttggcg tcaccggtgg catcgccgca tataaatcgg cggagctggt
     5581 tcgcgatctg caaaagcatg ccctcgacgt tcacgtcgtg atgaccgcgg ccgcgcaaca
     5641 gttcatcgcg ccgctcacct tcgccgctct taccgggcat aaggtcatca cgggcctctg
     5701 ggctccagtg ggttccgacg cgggcgatgc cacctccgcc atcgaacaca tcgatgctgc
     5761 acaatccacc gacgcgctcg tcgtcgcgcc agccaccgcc gacatcctgg cgaaattcgc
     5821 gcatggcatc gccgacgact tccttaccac gatgtacctc gccaccacag cgcccgtaat
     5881 cgtcgctccg gcgatgaacg tgaacatgtg gaaccatccc gcgacccagg cgaatctcga
     5941 ggttctcgcc agccgtcatg tgcgcatcgt cgccccggag agcggatatc tcgcctgcgg
     6001 catggtgggc tccgggcggc ttgctgagaa cagcgccatc gtcgacgcgg tgctgagcgt
     6061 gttgcttcat cgcaacgacc tcgccggcga aaccgttctg atcactgccg ggggcacgcg
     6121 cgaggctctt gacccggtcc gttttctggg caaccgttcg agcggcaaga tgggttacgc
     6181 catggcggaa gccgcgcagc gacgcggcgc gcgggtgatc ctggtttcgg cgccaaccgc
     6241 cttgcgaccg cccgcgaatg tcgaagtcgt tccggttgtg accacgcagc agatgcgcga
     6301 tgcggtgctt ggccgcctcg accaggcgac catcgtcatc aaagccgcgg cggtcgccga
     6361 ttatcgcccc cgcgctcagg ccgatcagaa gttgcgccgc aaaggcccgc tgacgctgga
     6421 actggagccg actgaagata tccttgccgc ggttgtcgcg cagcggcgcc ccggtatgct
     6481 ggtggtgggt ttcgccgcag agaccgaaga cgtgctgtcg cacgggcgcg ataagctgct
     6541 gcgcaaaggc gccgatgcca ttgtgctgaa cgatgtctcc cgcgaaggac ttggcttcga
     6601 ttcggaccgc aatgccgtca ccttcctcac tcttgatcgc gccatcgaat tgccggaact
     6661 gaccaagcgc gagcttgccg atcagattct cgatgagatc ctaaccctgc gcagcccacg
     6721 caaggaggtc ctctcggagt cggactccga ccttcccctg agtctcggag aacaggcttg
     6781 actgcacggt ttcccgcgga aacggaagag gtccttcgct cccgcattgc atattttcgc
     6841 gacctggggg tcttcgactt ctaccgccgc gaggtctccg cggccgataa tctgtcaaaa
     6901 cacatagata ctcttacaga gatcgccgat tccgctgcaa ttgcgaccgc tgttggtaca
     6961 gaacctggtg cggtcatgtc cgccgtattc cgttccgacg aagatctgga gaccgccgaa
     7021 gaaatgcccg ctgcgcgagt ccgcccgcaa cctctttccg tcctcaccca gtccacacca
     7081 cccgtcgccc ccgaagatcg agccgccgct cttcaggcga ttcgcgaaga cattggcaac
     7141 tgcacgcgtt gcgccctcca caaggggcgg cacacaattg tcttcggcga tggcgatccc
     7201 aacgcgcgcc tcatgtttgt aggtgaaggc ccaggcgccg acgaagacgc gcagggcctc
     7261 cccttcgtgg gccgggccgg acagctgctg aacaacatga tcggggctat ggggctccgc
     7321 cgcgaccagg tttacatcgc gaacatcgtc aaatgccgtc cgccgcagaa ccgcgtcccg
     7381 gaacccgtcg aggccaacac ctgtgcgccg ttcctgttcc gtcagatcga cgcaatccgc
     7441 cccgaagtga tcgtcgcgct cggtgcaacc gcagcgacgt acctgctcgg tggcaagagc
     7501 cctctcgccg cattacgcgg gcgcatccac cacgcgcgcg actcg
//
LOCUS       DASZYN010000638         9820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_228392, whole genome shotgun sequence.
ACCESSION   DASZYN010000638 DASZYN010000000
VERSION     DASZYN010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9820
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228392"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VHE33_16385"
     CDS             complement(<1..741)
                     /locus_tag="VHE33_16385"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HVT99084.1"
                     /translation="MKTELEALMRLQTIDGETATLRAAIAALPRRLAALETKLQGEKA
                     ALEQAQKAIKDEEALRRRSESDLKDQQQKIVKLRGQMSSVKTNEEYRAFQHEIEFAEA
                     EIKKIEDSEIESMERSEQLEQKRKAAESDLKDSTRAVEIEKEDARAESAEQQKKLEEL
                     TRRRAEERALVSEELLRTYDRVSTSSRGTGIAYAQGQRCTGCQMGIRPQMWNQIRAGE
                     ILTCESCGRLLYYDATREPQPEPVVEKPK"
     gene            complement(790..1248)
                     /locus_tag="VHE33_16390"
     CDS             complement(790..1248)
                     /locus_tag="VHE33_16390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HVT99085.1"
                     /translation="MRIRMTLFFPDLNVWLALSVEGHVHNTAASRWQRSMPGDSRLIF
                     SRYTQIGLLRLLTNSAMMGERVLTVREAWTVYDALLKHPQVEFYQEPRNIDTEFREVL
                     EPLASKSASKWVGDCWLLAFAAGSDAKLVTFDKALFDFARKWGQPAVMPR"
     gene            complement(1217..1447)
                     /locus_tag="VHE33_16395"
     CDS             complement(1217..1447)
                     /locus_tag="VHE33_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99086.1"
                     /translation="MAVRTTVDIPEPLHEQLRHRAASTGVSIRSLIVHALEQTYTKKR
                     KGRMAIEPLVRGKGKLGPRFPTDENPHDLIFS"
     gene            1464..2234
                     /locus_tag="VHE33_16400"
     CDS             1464..2234
                     /locus_tag="VHE33_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99087.1"
                     /translation="MFSALINGLNPNPDALLRAISAHISDDMLEEIARADYDEEVEGH
                     FAALLPIRNEARFIVPMQWCPGEVLELIRYSQPDRPDWKPGSPKIRGHWMRAFASAAL
                     LRAQFPPWNYRGDAAEPSLTLIQLIHSLRALPVDFNIEASRFVAYLMQQSNLEGDDEE
                     VLYYAISLIWFALHLDEPPTDESLIELCEWLVLREAQLAESLPGGFDRWLLGIRGAYP
                     PPSPWERFGPILAELDITAHSIQLQDWVRPIGSQLDEG"
     gene            2341..3945
                     /locus_tag="VHE33_16405"
     CDS             2341..3945
                     /locus_tag="VHE33_16405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HVT99088.1"
                     /translation="MPQDPDANWKPKHNPWAVALTVTLATFMEVLDTSIANVAIPHIA
                     GGLGASQDEATWVLTSYLVSNAVILPASAYLVGFLGRKRFYMICVALFGLSSMLCGLA
                     PSLPLLIFFRVLQGAGGGGLQPSEQSILADTFPPHKRGQAFAVYGMAVVLAPAIGPTL
                     GGWITDNYTWRWIFFINVPIAIFSLILTSRIVEDPPHIKREVAAQRKRGLNLDYMGFG
                     LLALGFGSLQFVLDKGQENDWFGSHVITTFFFLCAAALVTLIFWEIRQIRRGQRPILD
                     LTMFRNRNFSVSAMLMFVLGFALFGTTVLIPLFVQQMMGYTAEQAGLVISPGGIAILF
                     LMPLVGFLVGRVDNRYLIAIGFATLSAALYALRTIDAQASYSYVAWMRIFQASGLAFL
                     FVPINTLSYTDVPREKNNDVSGLMNLARNIGGSCGTSFFVTILARRSQFHQNRLGDSF
                     TGASIQLRQSIAGIASYLQHRGLTASPGQARALAQGSIYQQLIRQSTQLAYLDVIAIL
                     SIATVCMIPLIFLMKKRHPGAPAAAH"
     gene            4032..6380
                     /locus_tag="VHE33_16410"
     CDS             4032..6380
                     /locus_tag="VHE33_16410"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HVT99089.1"
                     /translation="MLCKIRPAFLILCLLGLSFAAQSPSLRAQADNPWTIQEIFGHPG
                     SLAGDPPEAISWSPDGKSATWIDPATGDLVEIEAPDPKPKKLLAYNKIAALLNASIPE
                     RDRDHRARYDQPDYIWAPDSEHLLFDTNGSLWLFTLKTGTGVQIGNSGSMSGDDPKFS
                     PSGQLLSYVRDGNLYVQRVDGSEQTIPLTNSSDNTILNGAADWVYLEELDVRSNYFWS
                     PDSREIAYLQMNEAAVPQYPLVDWIPVHANVEWQRYPQPGDPNPAVRLGIVSASGGAT
                     RWLKVPFDSGNDYIPRFGWVSPRIVWVETLSRNHQHLDLWFVDVRTGEFRRVLQQSDP
                     KYLTMTYDVKFVGDHTFFILSWRDGHTHIYRYTFDPGNPLSTLAQLANQVESGDYEVA
                     AIKAVDETNQTLWYLSSQGDPRQQQIWAVQFDGGNRRQISQEPGFHDPVFSPHGGSFI
                     DTYSSLLQPPTVAACFMASAPASVNCTPFWRSKPIQDHDILPPQQLQLKASDKTTTLY
                     GTILLPPGDSAPQSVPLIVNPYGGPGVGAARDRWGGKHFLFDQLLAEHGFAVLHVDNR
                     GMAGRGREFEQVAYHNFGPPQFADQMASIDEVLRQFPQINPHRLGWWGWSWGGSFTLF
                     ALSHSNSFLAGVAGAPVTDWRDYDSIYTERYLGLPSEDAQNYHDDSDVNSAADLKGHI
                     LILHGTGDDNVHIENDTQYIQKLIEAGIPYDYNIFPRKTHSVSGVDDQTELYAKILDH
                     FERYLMQPRPAEAQPGENKPADTKTPEAKPGERARTEQRARR"
     gene            complement(6716..7432)
                     /locus_tag="VHE33_16415"
     CDS             complement(6716..7432)
                     /locus_tag="VHE33_16415"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HVT99090.1"
                     /translation="MADLKGTSITWLGHATVLITTPKGTTILIDPFIEHNPKYPKGYK
                     LPKKIDLLLVTHGHMDHIADAIPVAKEHGPATIGMVEVIGWLHSKGLKDDQGIGMNVG
                     GSHRHADVTVSLTEARHSSGITDGDKTVYGGEPAGFVIAIDNGPTLYHAGDTTVFSDM
                     KLIGELYHPDLAMLPIGDHYTMGPRLAAVAAKYLGAKTILPIHWGTFPPLVGRPAELE
                     RELAGSGIGVIHTEPGQALS"
     gene            complement(7527..7976)
                     /locus_tag="VHE33_16420"
     CDS             complement(7527..7976)
                     /locus_tag="VHE33_16420"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HVT99091.1"
                     /translation="MTSFLGIATTLCIGLMIGTEFAVSAFINPVLEKLDNRTRVGIIR
                     LFAAKLGFAMPFWYCLGLLLLVAEAIIFRHGPQLPLLISAAGLWAAIIVLTLLFLVPI
                     NNRMMRLSPDTASGDDLREHKKWDSMHRIRVIALTGAMTLFLIAIHV"
     gene            8060..8413
                     /locus_tag="VHE33_16425"
     CDS             8060..8413
                     /locus_tag="VHE33_16425"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVT99092.1"
                     /translation="MPVSKKEISPCPIDAMLSVIDGRWKGTILWRLSNGPMRTSELRR
                     SIPEMTERMLIRHLQELVRDGIVLRKQESSVPPCVRYSISKYGWTLVPVLEVICAWGR
                     NHLRRACAAGRTAEN"
     gene            complement(8410..8928)
                     /locus_tag="VHE33_16430"
     CDS             complement(8410..8928)
                     /locus_tag="VHE33_16430"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HVT99093.1"
                     /translation="MPTTVLVTWATRYGSTEEVAHAVADDLLRQGLIVKAQPIAEVTS
                     LERYDAVVLGFALYMARMHKEARRFLKSHRDELMRRPVGVFVLGPIHATAKEFAEAER
                     QMNKELAKFPWFAPVARQVVGGRFSPEKLGFFFRNIPVLRNVPASDARDWNSIHAWAG
                     TLPDLLLPTGAR"
     gene            complement(9063..9473)
                     /locus_tag="VHE33_16435"
     CDS             complement(9063..9473)
                     /locus_tag="VHE33_16435"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HVT99094.1"
                     /translation="MGFIVWNDRLSVNVVTIDREHREWIDILNDLYDAVALGAGHEAV
                     SGTLARLTQYTEHHFQHEEALFSRTPYPDGKLDRRQHAEMVGWLSEWQRRYDAGDFSG
                     LSLEVVNYLKDWLFDHILGSDRQYTQYLHAAGIE"
     gene            9621..>9820
                     /locus_tag="VHE33_16440"
     CDS             9621..>9820
                     /locus_tag="VHE33_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99095.1"
                     /translation="MGEEKANIAPIPTLFPILGQTPVGIARLAAHGEPAGDGHLVQFR
                     TIPTRSILNRVDSKRVQWMSWS"
BASE COUNT         1873 a         3374 c         2561 g         2012 t
ORIGIN      
        1 cttcggcttc tccaccaccg gctccggctg cggctcccgc gtcgcgtcgt aatacaacaa
       61 tcgtccgcac gactcgcacg tcagaatttc tccggcgcgg atctggttcc acatctgcgg
      121 acgaatcccc atctggcatc ctgtgcaccg ctgcccctgc gcgtaagcaa ttccggttcc
      181 gcgcgacgag gtcgaaacgc gatcgtaggt ccgcagcagc tcttctgaaa ccagcgcccg
      241 ctcctcggca cgccgccgcg tcagctcctc gagtttcttc tgctgctccg cagactcggc
      301 ccgcgcatct tccttctcga tctcgaccgc gcgcgtcgag tccttcagat cgctctcggc
      361 agctttcctc ttctgctcca gctgctccga gcgctccatg ctctcgatct cgctgtcttc
      421 gatcttcttg atctccgctt ccgcgaactc gatctcgtgc tggaacgccc gatactcctc
      481 gttcgtcttc acgctggaca tttgcccgcg cagcttcacg atcttctgct gctggtcttt
      541 gaggtcgctt tcggagcgtc gccgcagcgc ctcttcatcc ttgatcgcct tctgggcctg
      601 ttccagcgcc gccttctcgc cctggagctt cgtttcaagt gcagccagcc gcctgggcag
      661 agccgcaatc gccgcccgca gcgtcgccgt ctccccatca atggtctgca gacgcatcag
      721 cgcttcaagt tcagttttca tctcggaatc tgcaggatat catccgttcc cgattgtttc
      781 cggtgaccgt caccgcggca tcaccgcagg ctgtccccat ttgcgcgcaa agtcaaataa
      841 cgccttgtca aacgtcacca gtttggcgtc cgatccagcg gcgaacgcca acaaccagca
      901 gtcaccgacc catttcgagg cagacttgct ggcaagcggc tccagaacct cgcgaaattc
      961 cgtgtcgata tttcgtggtt cctgatagaa ctccacctgc gggtgcttca gcaaggcatc
     1021 gtatacagtc cacgcctcgc gtacggtcag cactctctct cccatcattg cggaattcgt
     1081 cagcaaacgc agcaggccaa tctgggtgta gcgcgaaaaa atcaggcgcg aatcccccgg
     1141 catcgaacgc tgccaccgcg aggccgcggt gttatgaaca tgtccttcta ctgaaagtgc
     1201 cagccacaca ttgagatcag gaaaaaataa ggtcatgcgg attctcatcc gttgggaatc
     1261 gtggtcccag cttgcctttg ccgcgtacca acggctcgat tgccattcgg ccttttctct
     1321 ttttcgtgta ggtctgctcc agagcgtgca cgatcagcga acggatcgag acgcccgtgc
     1381 tcgcagcccg gtgccgcagc tgctcatgga gaggctccgg aatatcgaca gtggttcgaa
     1441 ctgccatgac ataattatgt cacatgtttt cagccctcat caatggcttg aacccgaatc
     1501 ccgacgcact gctgcgagcg atttccgcgc acattagcga tgacatgctt gaagaaatcg
     1561 cgcgtgccga ctacgacgag gaggtcgagg gccatttcgc tgctttgctt ccaatccgca
     1621 atgaagcccg tttcattgtg ccgatgcagt ggtgtcctgg tgaagtgctg gagctgatcc
     1681 gttattcaca gccggatagg ccagactgga aacccggtag tccaaagatt cgtggacatt
     1741 ggatgcgggc ctttgcaagc gccgcattgt tgcgtgctca gtttccgccc tggaattatc
     1801 gcggtgatgc tgccgaacca agcctgaccc tgattcagct tatccacagc cttcgcgctc
     1861 tcccggttga tttcaacatc gaggcatcgc ggttcgtcgc ctacctcatg cagcaatcta
     1921 atcttgaggg cgacgacgaa gaagtcctct attatgcgat aagcctgatc tggttcgcac
     1981 ttcaccttga cgagccaccg acagatgaat cgttaattga actgtgcgaa tggctcgtct
     2041 tacgcgaggc gcagcttgcc gaatccttgc ccggaggatt tgatcggtgg ctgctcggca
     2101 ttcgaggcgc ttatccaccg ccttcaccct gggaacgctt tggcccaatc ctcgcagaac
     2161 tcgatatcac cgcccactcg attcagttgc aggattgggt tcgaccaata ggttcgcaat
     2221 tggacgaagg ctgacttggc gctaacgaaa agttaaaact gcttcagatc ctttgcttgc
     2281 cgacgaccac cccacttttc ctgcatccac ctgctactct gaatcctcca acatccatcc
     2341 atgccccagg atcccgacgc caactggaag cccaaacata atccctgggc cgtcgcgctc
     2401 accgtcaccc tggccacctt catggaggtc ctcgacacct ccatcgccaa cgtcgccatc
     2461 cctcacatcg ccggcggcct cggcgcctct caggacgaag ccacctgggt cctgacctcc
     2521 tacctcgtct ccaacgccgt catcctcccc gccagcgcct acctcgtcgg cttcctcggc
     2581 cgcaagcgct tctacatgat ctgcgtcgcg ctgttcggac tcagctccat gctgtgtggc
     2641 ctggccccct cgcttcccct gctcatcttc ttccgcgttt tgcagggagc cggcggcggc
     2701 ggccttcagc ccagtgagca gtccatcctt gccgatacct tcccgcccca taaacgcggc
     2761 caggccttcg ccgtctacgg catggccgtc gtccttgccc ccgccatcgg ccctacgctc
     2821 ggcggctgga tcactgacaa ctacacctgg cgctggatct tcttcatcaa cgtccccatc
     2881 gccatcttct ccctcatcct cacctcacgc atcgtcgaag acccgcccca catcaagagg
     2941 gaagtcgccg cccagcgcaa acgcggcctc aatctcgact acatgggctt cgggctgctc
     3001 gccctcggat tcggctccct ccagtttgtc ctcgacaagg gccaggagaa cgactggttc
     3061 ggctctcacg tcattaccac cttcttcttc ctgtgcgccg ccgccctggt cacgctcatc
     3121 ttctgggaga tccgacagat tcggcgcggt cagcgcccca tcctcgacct caccatgttt
     3181 cgcaatcgca acttctcagt ctccgcgatg ctcatgttcg tcctcggttt tgcgctcttc
     3241 ggcactacgg tcctcatccc gctgttcgtc cagcagatga tgggctacac cgccgagcag
     3301 gccggccttg tcatctcccc aggcggcatc gccatcctct tcctcatgcc gctggtaggg
     3361 ttccttgtcg gccgcgtgga caatcgctat ctcatcgcca tcgggttcgc caccctctcc
     3421 gccgccttgt atgcgctgcg caccatcgac gcccaggcca gctactccta cgtcgcctgg
     3481 atgcgcattt tccaggcctc cggccttgcc ttcctcttcg tccccatcaa cacgctctct
     3541 tacaccgacg ttccgcggga gaagaacaac gacgtctccg gcctgatgaa tctcgcccgc
     3601 aacatcggcg gcagctgtgg cacctccttt ttcgttacca tcctggcccg ccgcagccag
     3661 ttccatcaga atcgcctcgg cgactccttc accggtgcca gcatccagct ccgccagtcc
     3721 attgccggta tcgccagcta tctccagcac cgcggcctca ccgcctcacc tggtcaggct
     3781 cgcgccctcg cccagggcag catctatcag caactgatcc gccaaagcac ccagcttgcc
     3841 tacctcgacg tcatcgccat cctctccatc gccaccgttt gcatgatccc tcttatcttc
     3901 ctcatgaaga agcgccaccc cggcgccccc gccgcggccc actgattcgc gccgcgttgc
     3961 cactccaaag gtttagaatt ctccttcacg aatcgatccg gcgccgccca ccgccgcctc
     4021 tggagacccg tttgctctgt aagattcgcc ccgccttcct gattctttgc ctgctcggtc
     4081 tctcgttcgc tgcccagtcc ccctctctgc gcgctcaagc cgacaaccca tggaccatcc
     4141 aggaaatctt tggccacccc ggctcgctcg ccggcgatcc tcccgaagcc atttcctggt
     4201 cccccgacgg caaatcggcc acctggatcg atcctgcgac gggcgacctg gttgagattg
     4261 aggcgcccga ccccaaacct aaaaagctcc tggcctacaa caaaattgcc gcgctcctca
     4321 acgcctccat tcccgagcgc gatcgcgacc accgcgcccg ctacgaccag cccgactaca
     4381 tctgggctcc ggactccgaa caccttctct tcgacaccaa cggctccctc tggctcttca
     4441 ccctcaaaac cggtaccggc gtccagatcg gcaactccgg ctcaatgtcc ggtgacgatc
     4501 ccaaattctc gcccagcggt cagctcctct cctacgtgcg tgacggcaat ctctacgttc
     4561 agcgcgtcga tggctccgaa cagaccatcc ctcttaccaa ctcgagcgac aacaccatcc
     4621 tcaacggcgc cgccgactgg gtctacctcg aagagctcga tgtccgcagc aactactttt
     4681 ggtcacccga ttcccgcgag atcgcctatc tccagatgaa cgaggccgcc gtcccgcagt
     4741 acccgctggt cgactggatt cccgtccacg ccaacgtcga atggcagcgc tacccgcagc
     4801 ctggcgaccc caatcctgcc gtgcggctcg gcattgtcag cgccagcggc ggcgcaacac
     4861 ggtggctcaa agttcccttt gactccggca acgactacat cccccgcttc ggttgggtta
     4921 gtcctcgcat cgtctgggtt gagacccttt cccgcaacca tcagcacctt gacctctggt
     4981 ttgtcgacgt ccgcaccggg gagtttcgcc gcgtcctcca gcaatcggat ccgaagtatc
     5041 tcaccatgac ctacgacgtg aaatttgtcg gcgatcatac cttcttcatc ctcagctggc
     5101 gcgacggcca cacccacatc tatcgctata ccttcgatcc cggcaacccg ctctccacgc
     5161 tcgcgcagct cgccaatcag gtcgagagcg gcgactacga agtggccgcg atcaaggccg
     5221 ttgacgagac gaaccagacc ctgtggtatc tctccagcca gggcgacccg cgccagcagc
     5281 aaatctgggc cgtccagttc gacggcggca atcgtcgcca gatttcccag gagcccggct
     5341 tccacgatcc tgttttctca ccgcacggcg gctccttcat cgacacgtat tccagcctgc
     5401 tgcaaccgcc cacggtggct gcgtgcttta tggcgtcggc gcctgcgtcg gtcaattgca
     5461 cgcccttctg gcgctccaaa ccgattcagg accacgacat tcttccgccc cagcagcttc
     5521 agctcaaggc ctcggacaaa accaccaccc tctatggcac catccttctg cctcccggag
     5581 actccgcccc gcaaagcgtt cctctgatcg tgaatcccta tggaggccca ggcgtgggcg
     5641 ctgcgcgcga tcgttggggc ggcaagcatt tcctcttcga ccagctgctt gccgaacacg
     5701 gtttcgccgt tctccacgtc gataaccgcg gcatggctgg ccgcggccgc gaattcgagc
     5761 aggtcgcata ccacaacttc ggcccgccgc agttcgccga ccagatggcc tccatcgatg
     5821 aggtcctccg ccagttcccg cagatcaatc cgcaccggct cggctggtgg gggtggagct
     5881 ggggcggtag tttcaccctc ttcgcgctgt cccacagtaa tagttttctc gccggtgttg
     5941 ccggcgctcc cgttaccgac tggcgcgact acgactccat ctacaccgaa cgctatctcg
     6001 gcctgccctc ggaggacgct cagaactatc acgacgactc cgacgtcaac tccgccgccg
     6061 acctcaaagg acacatcctc atcctgcacg gcacaggcga cgacaatgtc cacatcgaaa
     6121 acgacacgca atatattcag aagctcatcg aagccggcat tccgtacgac tacaacatct
     6181 ttccgcgcaa gacacactcc gtctccggtg tcgatgatca gacggaactg tatgcgaaga
     6241 tcctcgacca cttcgagcgc tatctgatgc agcccaggcc ggcggaagcg cagcccgggg
     6301 aaaacaaacc cgcggatacc aaaacgcctg aagctaaacc cggtgagcgc gcgcgaacgg
     6361 agcagcgcgc cagacgttga gcagtccgac aaccacaatc caggccagcc acaggccaaa
     6421 aatcgccaaa accaaaaccg gcgccaggcc aggttcaccc cacagcaaga agctcaaact
     6481 tctgtcgaac aaagatcaac agaaatcgca ccggatccca acccgaagta ccgttccgct
     6541 cccttttggg aatcgccctc gactagggct tcgaccgggt gatctggtac ctctcgcgaa
     6601 gcggtgtcgc gtcagcggca cgacggtaga agccccggcc ttcaggccgg ggaaaaagga
     6661 tccgcgggtt cgcagccttt aggcccggaa cgtactcaat cacgcggtca tggtactagc
     6721 tgagcgcttg ccccggttca gtgtgaatga cgccgatccc tgaccctgcc agttcccttt
     6781 ccagctcggc cggccttccc accagcggcg ggaacgttcc ccagtgaatc ggcaggatcg
     6841 ttttcgcgcc caaatatttc gcggcaacag cggccagtcg cggccccatg gtgtagtgat
     6901 cgccgatcgg caacatggcc agatccgggt ggtacagctc gccgatcagt ttcatgtcag
     6961 aaaagaccgt cgtgtcgcct gcgtgataca gcgtgggacc gttgtcgatc gcaatcacga
     7021 acccggccgg ctcgcccccg tagaccgtct tatcgccgtc tgttattccg gacgagtggc
     7081 gcgcctcggt gaggctgacc gtgacatcgg cgtggcgatg cgatccgcca acattcatcc
     7141 cgataccctg gtcatctttc agccctttgg aatgaagcca tccgatgact tcaaccatcc
     7201 cgatcgtcgc cggtccgtgc tctttcgcca caggaatcgc atcggcaatg tggtccatgt
     7261 ggccgtgcgt cacaaggagg aggtcgatct tcttcggtag tttgtagccc tttggatatt
     7321 tgggattgtg ctcgatgaac gggtcgatga ggatggtggt ccccttcggc gtcgtgatga
     7381 gaacggtggc gtgtccaagc cacgtgatgg atgtgccttt cagatcggcc atgggggttg
     7441 aagtcctccg cgcaatatcc tcgcactttc ttatgtctga tgcatgagcg ccatgcggcg
     7501 acacgcgcga atgcctctgg caggaactag acgtggatcg caatcagaaa tagcgtcatc
     7561 gccccagtca gggcgataac ccggatgcga tgcatcgagt cccatttttt gtgctcgcgg
     7621 agatcgtccc cgctcgcagt gtccggcgaa aggcgcatca tccggttgtt gatgggcacc
     7681 aggaacagca gggtaagaac gatgatcgca gcccacagtc cggccgcgga aatcagcagc
     7741 ggaagctgcg ggccgtgacg gaagatgatc gcttccgcga ccagcagcag cagccccagg
     7801 caataccaaa aaggcatggc gaaccccagt ttcgccgcga aaagacgaat gatcccaacc
     7861 cgcgtgcggt tgtcgagctt ctcgagcacg ggattaataa aggccgaaac ggcaaattcc
     7921 gttccgatca tcaggccgat gcaaagagtg gttgcgatcc cgagaaaaga ggtcatgttc
     7981 agctcctttg catgagacgc agcgccggct tacactgact catatgttcg tactgacaaa
     8041 ggtgtcagta gggagaagaa tgccggtctc aaagaaggaa attagtccgt gtcccatcga
     8101 tgccatgctg tcggtcatcg acgggcgctg gaaaggcacc atcctgtggc ggctttccaa
     8161 tggccccatg cggaccagcg agctccggcg cagcattcct gagatgaccg agcgcatgct
     8221 catccgccat ctccaggaac tggtgcgcga tggaatcgtg ctcaggaaac aggagagcag
     8281 cgtgccgccg tgcgtgcgct actccatttc aaagtacggc tggaccctcg tgccggtgct
     8341 ggaagtgatc tgcgcatggg gacggaacca tctgcgccga gcttgcgcgg ccggacgaac
     8401 agctgagaat taacgagcgc cggttggcag cagcagatcc ggcaatgtgc ccgcccacgc
     8461 gtggatcgag ttccagtcgc gcgcgtcgct cgccggaacg ttgcgcagca ccggaatatt
     8521 cctgaagaaa aagccgagtt tttccgggct gaatcgtccg cccaccacct gtcgcgccac
     8581 cggcgcgaac cacgggaatt tcgccagttc cttattcatt tggcgctcgg cctcggcaaa
     8641 ttccttcgcc gtcgcatgga tcggccccag cacgaacacg cccaccggtc ggcgcatcaa
     8701 ttcgtcacgg tgagacttca ggaagcggcg agcctctttg tgcatgcgtg ccatgtagag
     8761 cgcgaaccca aggaccaccg cgtcatagcg ttcgaggctg gtgacttctg cgatgggctg
     8821 cgccttcacg atgagcccct gcctcagaag atcgtcggcg actgcatggg cgacctcttc
     8881 cgtggatcca taacgcgtgg cccaggtaac gagcacggtg gtcggcatgg gaacctccgg
     8941 cgagcggcga agctgcgcga ggaaaccatc agcatgcaca ccgctcgacg gcggaagcat
     9001 ggcaccggtg ccggcggttg tcagtgaggc gggtcacaca agaatcaagg aagctgcgac
     9061 agctactcga tgcccgcggc gtgcaggtac tgggtgtact gccggtccga accgaggatg
     9121 tgatcgaaca gccagtcctt gagatagttc accacttcga gcgacaagcc ggagaaatcg
     9181 cctgcgtcgt accggcgctg ccattccgac agccacccga ccatctccgc gtgctgtcgc
     9241 cggtccagtt tgccatcggg atacggcgtc cggctaaaaa gcgcctcctc atgctgaaaa
     9301 tggtgctccg tgtattgggt caggcgcgcc agagttccgc tgactgcctc gtggccggcg
     9361 ccgagtgcga cggcgtcata gagatcgttg aggatgtcaa tccattctcg gtgctcgcga
     9421 tcgatcgtca ccacattcac cgacaggcgg tcgttccaga caatgaagcc catagctctg
     9481 ctgctcgttt ccgggcgctt gcgctcacca ggctacccaa ccgcgcaaac tactcgatga
     9541 aggtatcggc ggatttcctg aaaggagcag caaaagcgtc cagttgacgc agcacacccg
     9601 ccaggagtag cgtttcctcc atgggcgaag aaaaagcgaa catcgcgccg attcccactc
     9661 tcttcccgat cctcggccag accccggttg gcatcgcccg tctcgccgcc cacggcgagc
     9721 ccgccggtga tggccacctt gtccagttcc gcaccatccc cacccgctcc atccttaacc
     9781 gcgtcgattc caaacgcgtg cagtggatgt cgtggagcat
//
LOCUS       DASZYN010000639         4774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1252678, whole genome shotgun sequence.
ACCESSION   DASZYN010000639 DASZYN010000000
VERSION     DASZYN010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252678"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2461
                     /locus_tag="VHE33_16445"
     CDS             <1..2461
                     /locus_tag="VHE33_16445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HVT99096.1"
                     /translation="ALGRLQTPEQFRAIILRSNVDGSVLRLGDVARVELGQASYGTSA
                     LYNGKPASGFGVSLSTTGNALDAARSIEELLARLKPTFPAGVKSVVAYDTTPYVRKSI
                     VQVIKTLIEAVIIVIAVIFFFLQDWRATLIPAVTIPVSLVGTFAFIRVFGFSINSLTL
                     FGITLATGLVVDDAIVVIENVQRHIQEGDNEPHHATSAAMGEVTSAVIATSLVLIAVF
                     VPVSFFPGTTGILYRQFSLTIAFSIAISAFNALTLSPSLAALLLKRDDGKHTILHLAE
                     RSIKALGRGYASAIHGVLRIRWIMLLLFFVGLFGAYWEYTHVPTGFVPEEDQGFMMVL
                     VQAPQGASLAYTTGIEAQVSALLKQDKDVAGAFAVGGFSFSGNAPNFGMMFCSLAPYE
                     NREGPGHSAAEIIGRLQQKFFMIPGAMIYAFAPPAISGVGTLGGFQFELQDLGGNSIT
                     DLDRTAHLIVAKANQNPNLTRVLTSFTSNDPQVLVRIDRDKAKALGVPFSQISDALQV
                     YMGSAYVNNFDFNNRNYRVYVQAEQQFRQNASDIRQFYVRSSTGQMISLDNLVTIENT
                     SGPQVISHYNLFRSAEIDGNAAPGVSTSVALHSMEQVADQNMMHGMAYQWTGLALEEI
                     ESAGKAVVIFGLGILVVYLTLAALYESFALPFIILLAVPMAVLGALFAQSMRGYADDI
                     YCQIGLVMLIGLAAKNSILIVEFAEQLRAKGRSLVEAAVEAAELRLRPILMTSFAFIL
                     GVIPLAIASGAGAQGRRSVGTTIIGGMLVSTLLNLFFIPVLYIMLQTLLGKFGSGRSA
                     RRPAQSADTGAYVTSGSGTHS"
     gene            complement(2484..4487)
                     /locus_tag="VHE33_16450"
     CDS             complement(2484..4487)
                     /locus_tag="VHE33_16450"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HVT99097.1"
                     /translation="MANDSPLTQDQRVLHTLNRLTFGPRPGDVAAVEAMGLDKWIDKQ
                     LQPETIDDSVLEARLAAYPAMRLSRQELIRRFPSPAMIRQAEQGRRGLPGNPVERAIY
                     QDQIAALRNRQQAKGSRQQAATVAMRTQTDDMAPPPAPDMMPTNDSAKADEPSIEEQE
                     QKLYADLDAAKLVDLPPDQRVKTLVEMQPEEFRDFLRRLRRADRVALFTGLSPEQKET
                     VFALINPQLVVGGELLVTRVLRDVYSERQLEAVMTDFWLNHFNVYLRKGPFAPWYLVD
                     YQNKVIRPHALGKFEDLLVATAESPAMLFYLDQTQSVGPHSLAAMRAEWFQRGANGKA
                     RPVGLNENYARELMELHTLGADGGYTQRDVTEVAKIFTGWGIERPDEGGDFMFNPRRH
                     EPGSKAALGHRFGEGGESEGREVLHLLATSPVTAHHVSLQIAQRFVSDNPPEALVDRM
                     AATWVKTGGEIREVMRTMLESPEFWSKDAYRAKVKTPEEYVISAVRATGGDVVHPAAL
                     VEAIGQLGMPLYGCQTPNGYSWMADAWLNSGGLLDRMNFSMALADNNAGTVMNWDKLM
                     EIETQAGLTASAKENKLENLLLGGAVSEHTRSAALGQLAQQAGNDAGTPIGRGKMTRP
                     VNFVRPREFGQLLNAVAPLPPPADQDAALLGGLLLGSPEFQRR"
BASE COUNT          829 a         1728 c         1219 g          998 t
ORIGIN      
        1 ggcccttggc cgcctgcaga cccccgagca gtttcgggcg atcatcttgc gcagcaacgt
       61 cgatggctcg gtactgaggc tcggagatgt cgcgcgtgtc gagctcgggc aggcgagcta
      121 cgggacctcc gcgctctata acggcaagcc cgcttcggga ttcggtgttt cgctctcgac
      181 caccggcaac gcgctggacg cggcacgcag tatcgaggag ctgctcgcgc gcttgaagcc
      241 gactttcccg gcgggcgtca agtcggtcgt cgcctatgac acaacgcctt acgtgcgcaa
      301 gtcgatcgtg caggtgatca agaccctcat cgaagccgtc atcatcgtca ttgccgtcat
      361 cttcttcttc ctgcaggact ggcgcgccac gctgatcccc gccgtcacga tccctgtctc
      421 tctggtggga accttcgcat tcatcagagt cttcggcttc tccatcaact cgctcacgct
      481 gtttggtatc actctcgcca ccggcctcgt cgtcgacgac gctatcgtcg tcatcgaaaa
      541 tgtccagcgc cacatccagg aaggcgacaa cgagcctcat cacgccacct ccgccgccat
      601 gggcgaagtc accagcgccg tcatcgccac ctccctcgtg ctcatcgcgg tcttcgtgcc
      661 ggtctctttt ttccctggta ccaccggcat cctgtaccgt cagttctcgc tgaccatcgc
      721 tttctccatc gccatctcgg cattcaacgc cctcaccctg tcgccatcgc tcgccgctct
      781 gctgctgaag cgcgatgacg gtaaacacac catcctccac ctcgccgaac gcagcattaa
      841 ggcgctcggc cgcggctatg ccagcgccat ccacggcgtg cttcgcattc gctggatcat
      901 gctgttgctc ttcttcgttg gcctcttcgg cgcgtactgg gaatacaccc acgtccccac
      961 cggcttcgtc cccgaagaag accagggctt catgatggtg ctggtccagg ctccgcaggg
     1021 cgcttcgctc gcttacacaa ccggcattga agcccaggtg agtgccctcc tcaaacagga
     1081 caaggatgtt gccggcgcct ttgccgtcgg cggcttcagc ttctccggca acgccccgaa
     1141 cttcggcatg atgttctgct cgctggctcc gtatgagaac cgcgaaggcc ccggtcactc
     1201 tgccgctgaa atcattggcc gcttacagca gaagttcttc atgattcccg gcgccatgat
     1261 ctacgcgttc gcgcctcccg ccatctcagg cgtcggaact ctgggcggtt tccagttcga
     1321 gttgcaggat ctgggtggca actccatcac tgatctcgat cgcaccgcgc acctgatcgt
     1381 tgccaaagcg aatcagaatc cgaatctgac ccgcgtgctg accagcttta cctccaacga
     1441 cccgcaggtg ctggtgcgca tcgatcgtga caaagctaag gccctcggtg ttcccttcag
     1501 ccagatctcc gacgccctgc aggtctacat gggctcggcg tacgtcaaca acttcgactt
     1561 caacaaccgc aattaccgcg tctacgtgca ggcggagcag cagttccgcc agaacgcttc
     1621 ggatatccgt cagttctacg tccgctccag caccggccag atgatctcgc tcgataatct
     1681 cgtcaccatc gagaacacct ccggcccgca ggtgatcagc cactacaatc tcttccgctc
     1741 ggcagaaatc gacggcaacg ccgcacccgg cgtcagcacc agcgtcgccc ttcacagcat
     1801 ggagcaggtc gccgaccaga acatgatgca cggcatggcc taccagtgga ccggcctcgc
     1861 ccttgaagaa atcgaatccg ccggcaaggc cgtcgtcatc ttcggcctcg gcatcctcgt
     1921 cgtctacctc accctcgccg ccctctacga gagcttcgcg ctccctttca tcatccttct
     1981 tgctgttccg atggccgttc tcggcgcgct cttcgcgcag tccatgcgtg gctacgctga
     2041 cgacatctac tgccagatcg gactggtcat gctgatcggc cttgctgcca agaactccat
     2101 cctcatcgtc gagttcgccg aacagctccg cgccaagggc cgctctctcg tcgaggctgc
     2161 cgtcgaagcc gccgaactcc gcctgcgtcc catcctcatg acatcgttcg ccttcatcct
     2221 cggcgttatt cccctcgcca tcgcttccgg cgcaggagct cagggccgcc gctcggttgg
     2281 caccacgatc attggcggaa tgctggtttc cactttgtta aaccttttct ttattcctgt
     2341 gctctatatc atgctgcaga cgctgcttgg caagtttgga agtgggcgct ccgcgcgtcg
     2401 tccagcccag agcgccgata ccggcgccta cgtcacgagc ggctccggaa cccactcctg
     2461 atctcgggat cgagtcgctc gccttaccgt cgttgaaact ccggcgatcc caacagcagt
     2521 cctcccaaca gcgctgcgtc ctgatccgcc ggcggaggca gtggcgccac agcattgagc
     2581 aactgcccaa actctcgcgg acgcacaaaa ttcactggcc gcgtcatctt gccccgtcca
     2641 attggcgttc cggcatcgtt cccggcttgc tgagcgagtt gtcccagagc cgcactccgc
     2701 gtgtgctccg acaccgcacc ccccaacagc aaattctcca gcttgttttc cttcgcgctc
     2761 gccgttaagc ccgcctgcgt ctcgatctcc atcagcttgt cccagttcat caccgttccg
     2821 gcattgttgt ccgcgagcgc catgctgaag ttcatccggt ccagcagccc gccgctgttc
     2881 aaccacgcat cggccatcca cgaatagcca ttcggcgtct ggcatccata cagcggcatg
     2941 cccagttgcc cgatcgcctc caccagcgct gcaggatgca ccacatcccc gcccgtcgcc
     3001 cgcaccgccg agatcacgta ctcctccggc gtcttcacct tcgcccgata cgcatccttc
     3061 gaccagaact ccggcgactc cagcatcgtc cgcatcactt cgcgaatctc gccgcccgtc
     3121 ttcacccacg tcgctgccat gcgatcgacc agagcctccg gcggattgtc gctgacgaat
     3181 cgctgcgcaa tctgcaggct cacgtgatgt gccgtcaccg ggctcgtcgc cagcaaatgc
     3241 aatacctccc gcccttccga ctcgccgccc tctccgaatc gatgccccag cgcggccttc
     3301 gaaccgggct catgccgccg cggattgaac atgaagtccc caccctcatc cggccgctcg
     3361 attccccacc cggtgaagat cttcgccacc tccgtcacat cgcgttgcgt atatcccccg
     3421 tccgcgccca gcgtgtgcag ttccatcagc tcgcgggcgt aattctcgtt cagacctacc
     3481 ggccgtgctt ttccattcgc gcctcgctgg aaccactccg cccgcatcgc cgccagcgaa
     3541 tgcggaccca cactctgcgt ttgatcgaga tagaacagca tcgccgggct ctctgccgtc
     3601 gccaccagca ggtcttcaaa cttgccgagc gcatgcggcc ggatcacttt gttctgatag
     3661 tcgaccaggt accacggcgc gaacggcccc ttccgcaggt acacgttgaa atggttcagc
     3721 cagaaatcgg tcatcaccgc ttccagctgc cgctcgctgt acacatcgcg cagcacccgc
     3781 gtcaccagca gctccccgcc caccaccagc tgcggattga tgagcgcaaa caccgtctct
     3841 ttctgctccg gcgacaagcc cgtgaacagc gcgacgcgat ccgcccgacg taaccgtcgc
     3901 aggaaatcgc gaaactcctc cggctgcatc tcgaccagcg tcttcactcg ctgatccggc
     3961 ggaagatcga ccaacttcgc cgcatccaga tccgcgtaca gcttctgctc ctgttcttca
     4021 atcgacggtt cgtcggcctt tgccgaatcg ttcgtcggca tcatgtccgg agccggcggc
     4081 ggcgccatgt cgtcggtctg cgtcctcatc gcgacggttg ccgcctgctg cctgctgcct
     4141 tttgcctgtt gcctgtttct cagcgcggca atctgatcct ggtagatcgc ccgctccacg
     4201 ggattccccg gcagaccgcg cctcccctgc tcggcctgac gaatcattgc gggtgaagga
     4261 aaccgcctga tcagctcctg ccggctcagc cgcatcgctg gatacgcggc cagccgggct
     4321 tccagtacag aatcgtcgat cgtttccggc tgcagttgct tgtcaatcca tttgtccaac
     4381 cccatcgcct cgacggccgc cacatcgccg ggccgaggcc cgaacgtcag ccggttcagc
     4441 gtatgcagta cacgctgatc ctgcgtgagc ggcgaatcgt tcgccacagt caggggtgtc
     4501 ttctttttcc gtgcaaacgc cagaggctcg ccggccggta acacaagcag agcaacaagc
     4561 aacgcggaca atgaccgctg tattgcagca gaaagattgg tgggcatggc tggggatgtc
     4621 ctttagagac agtctgtcct ccccctcata acccgcgcaa ccattaaaaa gtcgcgcatc
     4681 ctgagggagc gcgcccagaa cgacttagat tgcccgaaag ggctccgctt taggtctctt
     4741 ccctactggc tattggctac tggctattgg ctac
//
LOCUS       DASZYN010000640         3443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_95595, whole genome shotgun sequence.
ACCESSION   DASZYN010000640 DASZYN010000000
VERSION     DASZYN010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95595"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VHE33_16455"
     CDS             <1..657
                     /locus_tag="VHE33_16455"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HVT99098.1"
                     /translation="GDLSAVQRWNDLRSQRLARPSLALHEREELLLARFLLASDRQVE
                     ALDLLEPLLSEARAAKRAQSMLELLIVLACARARAEQMDEAQEALREALELARAGKYI
                     RPFLNAGEVMARLLRVAFTRIGEKSLRAYLQTLLRAFVREPAASHVQEHTLPGEPLTS
                     RERQVLHLLAAGYSNPAIAHELVVSVHTVRTQVQSIYRKLDVHNRLQASELARLLHLL
                     "
     gene            824..2359
                     /locus_tag="VHE33_16460"
     CDS             824..2359
                     /locus_tag="VHE33_16460"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HVT99099.1"
                     /translation="MASSAQSNPEQSMDTDVIIVGAGPVGLFLAHELGLTGVRAVVLE
                     KLSARSGQSKATGVQPRTAEVLDLRGLLAELREQALPREAVGGHFAGLPVPLDCHAWN
                     TRYPYPLSIPQGRIEAFLAERLARYAIPLWWGQAIVGLEQDESGVTALVSGPGGETHL
                     RARYLVGCDGGHSTVRKLVGVNFPGRDGKSSMVVADVRLAGGAGKVPTRARHFSEHIR
                     RNGDVLGILSPLGDGVSRLLFGGPAQQTLARDAPITETEVRAALRASYGADVELQEIL
                     WASRFSDASRQVEHYQVGRVFLAGDAAHIHIPTGGQGLNLGLQDAFNLGWKLAAQVKG
                     QAPAGLLESYHTERHPVGARVLQNTRAQGLLMMLQSDEDGSALRELFIDLMRLPETNH
                     YLAGMISGLDVRYALPGADASPLIGRRMLDLDLSTPDGQTRVSQLLQGGRGLLLDLGR
                     HADLAAVAASSSVPVDYIQASTSADFAADAVLVRPDGYICWSAENASPDIESALHTWF
                     GSR"
     gene            complement(2559..>3443)
                     /locus_tag="VHE33_16465"
     CDS             complement(2559..>3443)
                     /locus_tag="VHE33_16465"
                     /inference="COORDINATES: protein motif:HMM:NF020768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0031221 - arabinan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinofuranosidase catalytic domain-containing
                     protein"
                     /protein_id="HVT99100.1"
                     /translation="NDNGNGHMDAINFSTECWFSPCSGSGPWVQADLENGLFAGGNGS
                     NTNNHGNSSAFVTALLKNNGQNRYAIKGGNAQSGSLTTWYNGAEPNRGGYAPMHQEGA
                     IVLGTGGDNSDGSVGSFFEGVMTAGYPTDAADNAVQANIVSVGYRSSSSFPVVGTAYR
                     LTNVNSGKVLDAVNCGTGNGTSVDLWAALGNTCQQWRFASAGSGIYTLTNVNSGKVLD
                     AVNCGWADGTLADLWSALGNKCQQWAVIPTGGGHYELVVENSGMVLDAQNCGTGNGTV
                     VRLWQWLNNPCQEWTIAP"
BASE COUNT          604 a         1055 c         1080 g          704 t
ORIGIN      
        1 ggcgatctct ccgctgttca acgctggaat gatctgcgtt cacagcgcct ggcaagacca
       61 tcgctcgccc tgcacgaacg ggaagaactg ctgctggctc gcttcctgct ggccagtgac
      121 agacaggttg aggcgctgga cctcctggag cctctgctca gcgaggcccg agcggcaaag
      181 cgcgcacaaa gtatgctgga acttctgatc gtcctggcct gcgctcgtgc tcgcgcggaa
      241 cagatggacg aggcgcagga ggctctgcgg gaggcgctgg aactggcgcg cgctggaaaa
      301 tacatccgtc cgttcctgaa tgcaggtgaa gtcatggcgc gcctgctacg cgtggccttc
      361 acccgaatcg gcgaaaagtc cctccgggcc tacctgcaaa ctttgttgcg cgccttcgtc
      421 cgggagccag cggcctcgca tgttcaggag cacaccctgc ctggtgagcc actcacttcc
      481 agggagcgcc aggtcttaca cctcctggcc gctggatact ccaatcccgc catcgcccat
      541 gaactcgttg tctctgtcca cactgtgcga acccaggtcc agagcattta tcgtaagctc
      601 gatgtgcata atcgcctcca ggcaagcgag cttgcgcgcc tgttacacct cctgtagccc
      661 gccagaaatc atccaccttc tcatgcagtt gagcgatgcg tctgccctgc acgatgcgct
      721 acactcctca tggagcgaga aaggatctga gcgatccgtg gcgacggtcc gcgcttttct
      781 cctctttttg cgcaatatca tgaaatgaaa ggaagtcctc tctatggctt catccgctca
      841 aagcaatcct gaacagagca tggatacaga tgtcatcatt gtgggagcgg gtccagttgg
      901 cctgttcctc gcgcatgaac tgggcctgac tggcgtgcgc gccgtggtcc tggaaaaact
      961 gtccgcgcgc agcggacaat cgaaagcgac cggggtacag ccacgcacgg cggaggttct
     1021 ggacctgcgc ggcctgctgg cggagctgcg ggagcaggcg ctgccccgcg aggcggttgg
     1081 cggtcacttc gccggcttgc cggtcccgct ggattgtcat gcctggaata cccgctatcc
     1141 ttacccgctg tcaattcccc agggacgcat agaagcgttt ctcgcagaga gactggcccg
     1201 gtatgccatt cctctctggt ggggacaggc aatcgtcggc ctggaacagg atgagtcggg
     1261 ggtgacggcc ctcgtcagcg gtccaggcgg cgagacgcac ctgcgggcac gctatctggt
     1321 tggctgtgat ggtggtcata gcacggtgcg taagcttgtc ggcgtcaact ttcccggtcg
     1381 agacggcaaa tcgtccatgg tcgtggccga tgtcaggctg gccgggggag caggaaaagt
     1441 gcccacgcgc gccaggcact tcagtgagca tattcggcgc aatggcgatg tcctgggcat
     1501 cctttctccg ctcggcgatg gcgtcagccg tctgctattt ggcggtcccg cacagcagac
     1561 gcttgcccgt gacgcgccga tcactgaaac ggaggtgcgc gccgctttgc gtgcctccta
     1621 tggtgccgat gtcgagttgc aggaaatcct gtgggcatcc cgcttcagcg acgcctcacg
     1681 ccaggttgag cactatcagg taggccgggt cttcctggca ggcgatgcgg cccatatcca
     1741 cattcccacc ggtggccagg gtctcaacct gggcctccag gatgcgttca atctgggctg
     1801 gaaactggcc gcgcaggtga aaggccaggc acccgcagga ctcctggaga gctatcacac
     1861 ggagcgccac ccggtaggcg cgcgggtact tcagaacacg cgcgcgcagg gtctgttgat
     1921 gatgctccag agcgacgagg atgggtccgc cctgcgcgaa ctgttcattg acctgatgcg
     1981 cctgccggag acgaaccact acctcgcggg catgatctcc ggtctcgatg tgcggtatgc
     2041 tctgccgggc gcggacgctt cgccgctgat tggccggcgt atgctcgatc tggatctgtc
     2101 caccccggac ggtcagacgc gcgtcagcca gctcttgcag ggcggtcgcg gtctgcttct
     2161 ggacctgggt cgtcacgccg atctggccgc cgtcgcagcc agttcgtcag tgccagtgga
     2221 ctacatacag gccagcacgt ccgctgattt tgccgccgat gccgtcctgg ttcgtcccga
     2281 tggctacatc tgctggagcg cagaaaacgc cagccccgac atcgagtccg cgttacatac
     2341 ctggtttggt tcgcgctagc tccagttgca cacgcaggcc catcgcggcg tcgcttgctg
     2401 cctgtatctt gtggctatag gaaatattct gggcagagag ggatgacgct gtacgtagtt
     2461 gtgaagcgtc atccctctcg gtgtctcccc cattgtctca gacgggcaac atatgagagg
     2521 ctcgcgacaa tggaaaagac atcggcgaag aggtgagatc agggggcgat ggtccattcc
     2581 tggcaggggt tgttgagcca ttgccagaga cgaacgacgg taccgttgcc ggtgccgcag
     2641 ttctgcgcgt cgagcaccat gccgctgttt tcgacgacca gctcatagtg cccgcctccg
     2701 gtgggaatga cggcccactg ctggcatttg ttgccaagcg cagaccacag atccgccagc
     2761 gtgccgtccg cccagccaca gtttacggcg tccagcactt tcccgctgtt gacgttggtg
     2821 agggtgtaaa tgccgctgcc ggcgctggcg aatctccatt gctgacaggt gttgccgagc
     2881 gcggcccaca gatcaaccga ggtgccattg ccggtgccac agtttacggc gtccagcact
     2941 ttcccgctgt tgacgttggt cagacggtag gcggtgccga ccacggggaa gctgctgctg
     3001 cttctatagc caacggagac gatgttggcc tgcacggcgt tgtccgccgc gtcggtggga
     3061 taaccggcgg tcatgacgcc ctcaaagaag gagccaaccg agccgtcgct gttgtcgccg
     3121 ccggtcccca ggacgatggc gccttcctgg tgcatgggag catagccgcc ccgattcggt
     3181 tccgcgccgt tgtaccacgt ggtcagacta cccgactggg cgttgccacc tttgatggcg
     3241 tacctgttct ggccgttgtt tttgagcaaa gcggtgacaa aggcgctgga gttgccatgg
     3301 ttattggtgt tggagccatt gcctccggca aaaaggccgt tttccaggtc cgcctgcacc
     3361 cagggaccgg agccggaaca cggggagaac cagcattcgg tgctgaagtt gatggcgtcc
     3421 atgtggccgt tgccgttgtc gtt
//
LOCUS       DASZYN010000641         2467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_95629, whole genome shotgun sequence.
ACCESSION   DASZYN010000641 DASZYN010000000
VERSION     DASZYN010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95629"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(332..1048)
                     /locus_tag="VHE33_16470"
     CDS             complement(332..1048)
                     /locus_tag="VHE33_16470"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HVT99101.1"
                     /translation="MASFPFLRRPFALPSALRRGTKAYDLLAGAPLVLFYAVGANGKA
                     QSLIEIFSKLDPEKLDLATVLTVLSDIAALSVALLFVVCVLLRPPPIARAGGLMPRIA
                     AVVGTYMAVGLILLSPVTKASVGIVCLSLFLVLCGTSFALWAVFYLGRSVSLMAEARK
                     LVTGGPYRFVRHPLYLGEQISIAGVLLPYLSPIAAFVFAVQIAFQFYRMRCEEEILAA
                     TFPEYADYAKRTWRVLPGLY"
     gene            complement(1094..>2467)
                     /locus_tag="VHE33_16475"
     CDS             complement(1094..>2467)
                     /locus_tag="VHE33_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99102.1"
                     /translation="SMCQYPAQPTISSCPTPDPASKIVALKTATHNLLQMLQNAASND
                     GDVKVSIVPFSKDVNVGTGHVGDNWIDWSDWEAPPPSSMPGSNVGPGSSCPYGTSTSP
                     FGYSCINTQSGKIASSGTYKGMICPGTDNGRYNSGDGSTVNDNSQHQYNGCYNSTPTK
                     TLVTTVTKTVPSYATYSCQTVNGGTESCSLTGTSTGSTQTDTNTQTVAGYSGDSSTST
                     NSTSGPTTTDGSKSCNTRRGTTTCTWTRKIETDAITTTVTNTGAAPFNHAWVVNDHST
                     WGGCIMDRAQDYDTLNTAPSGSAKFPAENSNYCLPGTTTPLSSSWSTLSSQVDAMTAN
                     GNTNQTVGLAWGWQTLTNSDPYNPGEVPEDTRKVLILLTDGLNTQNRWSGDASTIDAR
                     TKAICQNIKDDKITVYTVLVMAGSSSMLKGCASDDSKYFELTSADEIVTTFQQIGTQI
                     TNLRVSL"
BASE COUNT          473 a          705 c          773 g          516 t
ORIGIN      
        1 ggtggggttg gttttttcga tctcggccgt aatgatcctc attggatctg tacatcttgc
       61 ctggcactac gcagtcgacg ggctcgcggg tgcgggactt gccgttctgt gctggtgggg
      121 cgcgggatgg accgcgcggc gcgcacacgc tgcggttaga cgagacacag attggccaac
      181 agttccggaa gccgtttagt ttccgccacg accgctcgtg tgccagcctc tcctacagcg
      241 tgaccatcga ctagcgcagc cagccgctac aattgaagtg gtgcttggtt ggttccgacg
      301 cgcggaacca ggcgtatgcc aacaagtatc ctcagtagag gcccggtaaa acccgccagg
      361 tgcgcttcgc ataatcggca tactccggga acgtcgcggc aaggatctcc tcctcgcacc
      421 tcatcctgta gaattgaaat gcaatctgca ccgcaaacac gaaagccgct atcggcgaaa
      481 gatacggcag gagcacgccg gcaatcgaaa tctgttcgcc cagataaagc ggatggcgca
      541 caaagcgata gggaccgcca gtcacgagct tgcgtgcttc ggccatgagg cttaccgaac
      601 gcccgagata gaacaccgcc cacaaggcga agctcgtgcc gcaaagcacc aggaacagcg
      661 aaagacagac gatgcccacc gatgccttcg tgacgggaga aagcaatatg aggcccacag
      721 ccatataggt tccgacaacc gcggcgatgc gaggcatcag cccgccggcg cgcgcgatgg
      781 gcggcggtct gagcagcacg cagaccacaa acagcaacgc cacggacaag gctgcgatat
      841 cgctgagcac ggtgaggacg gtcgcgagat cgagtttctc cggatcgagc ttggaaaata
      901 tttcgatcag cgattgcgcc ttgccgttgg cgccgactgc gtagaacagg acaagcggcg
      961 caccggcgag aaggtcataa gccttcgtgc cgcggcgcag cgcagacggc aacgcaaaag
     1021 gccggcggag aaacggaaac gaagccatcg gtgcacgcta gcgggcaaat cttaaggatt
     1081 tgcccgccgt ctcctacagc gacacccgca ggttggtgat ctgggttccg atctgctgga
     1141 aggtcgtcac gatctcatcg gcgctcgtca gttcgaaata tttgctgtcg tccgacgcgc
     1201 agcctttcaa catgctcgac gaaccggcca tcaccagcac cgtatagacc gtgatcttgt
     1261 cgtctttgat gttctgacag atcgccttgg tgcgggcgtc gatcgtgctc gcgtccccgg
     1321 accagcggtt ttgcgtgttc aagccgtcgg tcagcagaat gagaaccttg cgcgtgtcct
     1381 ccggcacctc gccgggattg tagggatcgg aattggtcag cgtctgccag ccccaggcca
     1441 gtcccaccgt ctggttggtg ttgccgttcg cggtcatcgc atcgacctgg ctcgacagcg
     1501 tgctccagct ggaagagagc ggagtggtcg tccccggcag gcagtagttg gagttctccg
     1561 ccggaaattt cgccgaaccg gagggcgcgg tgttcaacgt gtcgtagtcc tgcgcgcggt
     1621 ccatgatgca gccaccccaa gtcgagtggt cgttcacgac ccaggcgtgg ttgaagggag
     1681 cggcaccggt gttggtcacc gtggtggtga tggcgtcggt ttcgattttt ctggtccacg
     1741 tgcacgttgt cgtgcccctt ctggtgttgc agctcttact gccgtccgtc gtcgtgggtc
     1801 cggaggtcga attggtactg gtgctgctgt cgccgctata accggcgacg gtctgcgtgt
     1861 tggtgtcggt ttgcgtcgat cctgtcgaag tgcccgtcag agaacagctt tccgttccgc
     1921 cattgaccgt ctggcagcta taggtggcgt aggacgggac ggttttcgtg actgtcgtaa
     1981 ccaacgtttt cgtcggcgtg ctgttgtagc agccattgta ttgatgctgt gaattgtcat
     2041 tgacggtaga gccgtcaccg gaattgtacc ggccgttgtc ggtgccgggg cagatcatcc
     2101 ctttgtatgt gccggacgag gcgatcttgc cgctttgcgt gttgatgcag ctatagccga
     2161 acggactcgt gctcgtgcca taagggcaac tcgaacccgg tcccacattc gaaccgggca
     2221 ttgaactggg cggcggagct tcccagtcgc tccagtctat ccagttatct ccgacatggc
     2281 ccgttccgac attgacgtcc ttgctgaacg gaacgatcga cactttcaca tcgccatcgt
     2341 tggaggcagc gttctggagc atctgcagga gattgtgcgt cgcggtcttg agcgccacga
     2401 tcttggaagc cggatccggc gtcgggcagg aactgatggt gggttgggcc gggtattggc
     2461 acatcga
//
LOCUS       DASZYN010000642         6008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_863388, whole genome shotgun sequence.
ACCESSION   DASZYN010000642 DASZYN010000000
VERSION     DASZYN010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863388"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /gene="lpxB"
                     /locus_tag="VHE33_16480"
     CDS             <1..859
                     /gene="lpxB"
                     /locus_tag="VHE33_16480"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HVT99103.1"
                     /translation="PVIYFVSPQLWAWKKYRIRSVQRYVDRMLTIFPFEERFYHDHGV
                     DAEFVGHPLAECDLPTITRAQFAQQHNLDPAKTWVGLLPGSRGKEIRQNLPEMVRAAA
                     LLDPGTQFLLPLAPTLTGPQRDHIRALLAALLRSEPQPPKIALTDDARAVLHLAQASI
                     VASGTATVEAALIGNPFVVVYRISPLSYAVAKRVVDVPHVAMVNLIAGKRIVPELIQG
                     DFTAAAVVSHLRPLLHDREARVRMQGNLRRVGEMLRAQQSAAGRVAQIACEMLKPRPT
                     PEERLSQIS"
     gene            886..2895
                     /locus_tag="VHE33_16485"
     CDS             886..2895
                     /locus_tag="VHE33_16485"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HVT99104.1"
                     /translation="MTHAPLWCRFSLRRFTSLAAACWMAAFLPCLPSAAQNVASKPTL
                     DITGYNINVTVHPADHSLDATTQVTFTPLADLPTAIFDLHGALLVDKVTDASGATLNG
                     QRGPGATLVVTPAAPLTQGQSMTWTFHYSGKLESDAGGPVEGLKLAYVGDPISYLLYA
                     GRWFPMTGYMTDRFTADIHVKAPAGYRVIGSGSTGKPASAAGGAEEWDFKWDKPGFPG
                     TIILGKFEPPISGAAPNIHVYTTAAHKAAATDYAGTALREFAYFTSTFGLPESTQLNV
                     VELPDDTVPVAWAPEIAAVAGAHIGGKNDFRLLANTIAHQWWGSEVSPAALNDAWIVN
                     GMARYSELMYLEDDAGHGALQSALPDIAASALAYDTIPLSSAGTLAAYSPQFQSMTLD
                     KGAMVFHMLRWEVGDDSFEKILRATLTQYKDKGIKTSEFTRLAEEQSQQQLTPFFSEW
                     LDGTGAPQFTNKYAVYRVGNNKGFRTIGEIQGDMDLFNMPIDLRIETEGKTVDKRVDV
                     VGTDSQYVVDTFGRPRRVVIDPQDWILKNTPELEVRVAILRGQQLAAQGDNGGALTEY
                     QKALKANPQSSLASYRIGELLFSMRNYQASVNAYRDALNGDGEPKWTEVWSHVAIGKI
                     FDLTGQRDRAVQEYRQAVQTNDNTAGALNEARHWIQTPYKRPDTE"
     gene            complement(2919..3206)
                     /locus_tag="VHE33_16490"
     CDS             complement(2919..3206)
                     /locus_tag="VHE33_16490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HVT99105.1"
                     /translation="MAKAYAIHPGEILREEFLKPMGLTAYRLAKDISVPLPRVHDVVR
                     GRRGITADTAIRLGIYFGLPAQFWLNLQNDYDLRLAARKGAHSQIRPRRAA"
     gene            complement(3555..4343)
                     /locus_tag="VHE33_16495"
     CDS             complement(3555..4343)
                     /locus_tag="VHE33_16495"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT99106.1"
                     /translation="MDTGLKGRVAIVAGSSAGIGKATALAFAAEGANLALCARRQAEL
                     DQVAEEARSRFGVETFTQTFDVGDPAGVQAFVEAAYARFGRVDVCVPNAGGPPAKPFL
                     DTTLEEWERTWHLNLRSAIVFAQAVLPHMQKQHWGRIVTLTSYTVKQPVPELVLSNTI
                     RAGMMGLVRSLAGQFGKDGITINNVGPGFTATDRSRSLLETRAKSRNISVEQVKSELE
                     REIPIGRMAKPEEVAETIVWLASEAAASITGQTILVDGGSYKGL"
     gene            4406..4774
                     /locus_tag="VHE33_16500"
     CDS             4406..4774
                     /locus_tag="VHE33_16500"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVT99107.1"
                     /translation="MSDTTELRHTSFDKIALEHLNPLIDRQFVHGERTMLARVILRKG
                     AIVPRHSHENEQITYILEGALRFTMGDGQVLTVGAGEVLVIPSNMPHQAEALEDTVDL
                     DVFAPPRADWIAGTDAYLRK"
     gene            complement(4813..>6008)
                     /locus_tag="VHE33_16505"
     CDS             complement(4813..>6008)
                     /locus_tag="VHE33_16505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVT99108.1"
                     /translation="GFVRRSRVGHSSPRGDRTAMKPAIDPAFAFHVGLTGNLGSSAVM
                     FYPASLLGWVLLFFLVLFLSGAFVLAWRFAKSRLHGPGEQFGGELELLRAVMGSVPDL
                     IYVKDLESRFLFANQGTADVMGAESGQELRGKSDFDYYPSELAAGFYQDEQKVIQTGI
                     PLVSHDEHVREVDGRIRWLLTTKVPFRDPEGKTVGIIGIGRNITALKETEAELKRARE
                     NLHFKATHDSLTSLLNREAILDMLGRELARGAREQGSAAVLLADLDHFKSVNDRFGHP
                     VGDEVLRETARRFLRSVRGYDLVGRLGGEEFLVILGGCGAQEALTRADQLRLSIEESP
                     MPTTCGPLSLTISIGVLPAREWGYPRREEVLREVDVALYAAKAEGRNRCRLAVPPDDV
                     SATSI"
BASE COUNT         1173 a         2109 c         1645 g         1081 t
ORIGIN      
        1 gcccgtgatc tacttcgtca gcccgcaact ttgggcctgg aagaaatacc gcatccgcag
       61 cgtccagcgt tacgtcgacc gcatgctcac catctttccc tttgaagagc gcttctacca
      121 cgaccacggc gtcgacgccg aattcgtcgg ccatcccctc gccgagtgcg acctgcccac
      181 catcacccgc gcccagttcg cccagcagca caacctcgac cccgccaaaa cctgggtcgg
      241 cctcctgccc ggcagccgcg gcaaagagat tcgccagaac ctccccgaga tggtccgcgc
      301 cgccgccctg ctcgacccgg gcacgcaatt cctgctcccc ctcgccccca ccctaaccgg
      361 cccccagcgc gaccacatcc gcgcccttct cgccgcactt ctcagatccg aaccccagcc
      421 gcccaaaatc gcccttaccg acgacgcccg cgccgtcctc cacctcgccc aggccagcat
      481 cgtcgccagc ggaaccgcaa ccgtcgaggc cgccctcatc ggcaacccct tcgtcgtggt
      541 ataccggatt tcaccgctga gctacgccgt cgcgaagcgg gtggtggatg taccccacgt
      601 ggctatggtg aacctcatcg ccggaaaacg catcgtgccc gaactgatcc agggtgactt
      661 tacagccgct gctgtcgtct cacacctcag accgttgctg catgacaggg aagcgcgggt
      721 ccggatgcag ggaaacctgc ggcgcgtggg agagatgttg cgtgcccagc aaagcgctgc
      781 cggccgcgtc gcacagatcg catgcgagat gctgaaacca cgacccacgc cggaagaaag
      841 actgagccag atttcatgaa gtcaggttcc atgaaggatt tccctttgac ccacgcgccc
      901 ctgtggtgcc gtttttcgtt gcgccgcttc acgtctctgg ccgcggcttg ttggatggcc
      961 gccttcctgc catgccttcc gtccgccgcc cagaacgtcg cttccaaacc gacgctggac
     1021 atcacgggat acaacatcaa cgtcacggtc catcccgccg accattcgct ggacgcgacg
     1081 acgcaggtca cattcacgcc tctggctgac ctgcccaccg ccatctttga ccttcacgga
     1141 gccctgctcg tcgacaaagt cacagacgca agcggtgcga cgctgaatgg ccagcggggg
     1201 ccgggcgcaa cgctggtggt aacgcctgca gcacccctga cccagggcca gtccatgacc
     1261 tggacctttc actattccgg caagctcgaa tccgatgccg gcggtccggt cgaggggctg
     1321 aagcttgctt acgttggcga tcccatcagc tatttgttgt atgcgggccg ctggtttccg
     1381 atgaccggct acatgaccga tcgcttcacg gcggacattc acgtcaaagc cccggcgggc
     1441 tatcgcgtca tcggcagtgg cagcaccggt aagcccgcgt ccgctgcagg cggtgcggag
     1501 gaatgggact tcaagtggga taagccgggt tttcccggca cgatcatcct tggaaaattc
     1561 gagccgccca tctcgggtgc cgcgcccaac atccacgtct acaccacggc tgctcacaag
     1621 gctgcggcga ccgactacgc cggcaccgct ctgcgcgagt tcgcctactt caccagcacc
     1681 ttcggattgc ctgaatcgac ccagctcaac gtcgtcgagc ttcccgacga tacggttccc
     1741 gtcgcctggg caccagagat cgcggccgtt gccggcgccc acatcggcgg caagaacgac
     1801 ttccgcctgc tcgccaatac catcgcccac cagtggtggg ggagcgaagt cagccctgcc
     1861 gccctcaacg atgcctggat cgtcaacggc atggcgcgct acagcgagct gatgtacctc
     1921 gaagacgacg ccgggcacgg tgcgttgcag tctgccctgc cggatatcgc ggccagcgct
     1981 ctcgcctacg acaccatccc gctttccagc gccggcactc ttgcagctta ctccccgcag
     2041 ttccagtcca tgaccctcga caagggggcc atggtcttcc acatgctccg ctgggaagtc
     2101 ggcgacgaca gcttcgagaa gattctgcgt gccaccctca cccagtacaa agacaagggc
     2161 atcaaaacca gcgaattcac acgtcttgcc gaggagcagt cgcagcagca gctcactccc
     2221 ttcttctcag aatggctcga cggtaccgga gcgccgcaat tcaccaacaa atacgccgtc
     2281 tatcgcgtcg gcaacaataa gggtttccgc accatcggcg agatccaggg cgatatggac
     2341 ctgttcaaca tgcccatcga tctgcgcatc gaaaccgaag gcaagaccgt cgacaagcgc
     2401 gtcgacgtgg ttggtactga ctctcagtac gtggtcgata ccttcggccg cccgcgccgc
     2461 gtcgtcatcg atcctcagga ctggatcctg aaaaacaccc cggagctcga ggtccgggtc
     2521 gccatccttc gcggccagca actggcggct cagggcgata acggcggtgc tctgaccgag
     2581 taccagaagg cgctcaaggc caatccgcaa agttcgctgg ccagttaccg catcggcgag
     2641 ctgctgttca gcatgcgcaa ctaccaggcc tccgtcaacg cctatcggga tgcgctgaac
     2701 ggtgacggcg aaccgaagtg gaccgaggtc tggagccacg tcgccatcgg caagatcttt
     2761 gatctcaccg gccagcggga ccgtgccgtt caggaatacc ggcaagccgt gcagaccaat
     2821 gacaacactg ccggggcact caacgaggcc cgccactgga tccagactcc ctataagaga
     2881 ccggacaccg agtagcggag tttagtcggt aactggcctt aagcggcccg ccgcggcctg
     2941 atctggctgt gggcgccttt gcgggcggcc agcctcaagt catagtcgtt ctgcaggttg
     3001 agccagaact gagcaggaag accaaagtag atcccaagcc ggatcgccgt gtccgccgta
     3061 atgccgcgcc gcccccgaac gacatcgtgg acgcggggaa gcggtacgga gatgtctttc
     3121 gccaaacggt aagctgtaag ccccatcggc ttgaggaact cctcccgcag aatctctccg
     3181 ggatgtattg cgtatgcctt cgccatacca cccacctcag tgatagtcga cgatctcgac
     3241 gtcaaatgca tttccgtcgg accagcaaaa gcagattcgg tgctggtcgt tgatgcggat
     3301 taaatatcga tctgcacggt ctacgctgag cttttccaga cggtttcctg gaggcacagt
     3361 caggttttcc agagccagcg cggcgttgag aattgcaagc ttgcgaaaac atgcttttcg
     3421 caggtttgcc ggaaaccgtc gactcttacc ggttcgccag agcagctcgg tttccggatt
     3481 tcgaaagcca acgatcacaa ggcgatgcta acgactgtcg ttaacgtcaa tcgttactat
     3541 ggtgccgaat ggcctcacaa ccccttgtaa cttcccccgt ccaccaggat cgtctgtccg
     3601 gtgatgctgg ccgccgcctc cgacgccagc cacacgatgg tctccgctac ctcctcaggc
     3661 tttgccatcc gcccaatcgg aatctcccgc tccagctcgc tcttcacctg ctctacggag
     3721 atgttgcggc tcttcgcccg cgtctccagc aggctccgcg agcgatccgt agccgtaaaa
     3781 ccaggcccca cattgttgat cgtgatgccg tctttgccga actgtccggc cagcgagcgc
     3841 accagcccca tcatccccgc gcgaatcgta ttcgacagca ccagctccgg caccggctgc
     3901 ttcaccgtgt aggaagtcag cgtcacgatg cgcccccagt gctgcttctg catatgcggc
     3961 aacacagcct gcgcgaagac aatggcgctg cgcagattca ggtgccaggt ccgctcccac
     4021 tcctccaggg ttgtatcgag aaatggtttc gcaggaggtc cgcccgcatt gggtacgcaa
     4081 acatcgacgc gcccaaatcg cgcgtaggcg gcctcaacga atgcctgcac gccggccgga
     4141 tcgcccacat cgaacgtctg cgtgaacgtc tccacaccaa agcggctccg cgcctcctcc
     4201 gccacctgat ccagttccgc ctgccgccgt gcacacaacg ccagatttgc gccctcggcc
     4261 gcaaacgcca gggccgtcgc ctttccaatg cccgcgctgg agccggcaac gatggcgaca
     4321 cggcctttga gtcccgtatc catgcgccct atgatagtcg agtcaaaatt ccccgatccc
     4381 gttccatctt tcccggagac ccaacttgtc cgacacaacc gaactgcgcc acacaagttt
     4441 cgacaaaatc gctctggagc acctgaaccc tctgatcgat cgccagttcg tgcacggcga
     4501 gcgcacgatg ctggcacgcg tgattctacg caagggcgcc atcgttcccc gccacagcca
     4561 cgagaacgaa cagatcacct acatcctcga aggcgctctc cgattcacaa tgggcgacgg
     4621 tcaggtcctc accgtcggcg cgggtgaggt cctggtcatc ccttcgaaca tgccccacca
     4681 ggccgaagcc cttgaggaca ctgtcgacct cgatgtcttt gcgccgcccc gtgcggactg
     4741 gattgctggt acagacgcct atttgaggaa atagcagccc gcgtcgcgcc tcccgcggcg
     4801 gccgagccag cctcaaatcg aggttgcgga cacgtcgtcc ggcggtaccg ccagcctgca
     4861 gcggttccgc ccctctgcct tcgcagcgta caaggccacg tcgacttcgc gcagcacttc
     4921 ctcccgtcgc ggataccccc attcgcgcgc aggcagtacc ccgatgctga tggtcaggga
     4981 cagcggaccg cacgtcgtcg gcatgggcga ctcctcgatg ctcaggcgca gctgatccgc
     5041 gcgcgtcagc gcctcctgcg cgccgcagcc gcccaggatg acaaggaact cctccccacc
     5101 cagacgccca accaggtcgt acccgcgcac ggagcgcaga aatcgtcgcg ccgtctcgcg
     5161 cagcacctca tcgcccactg ggtgcccaaa tcggtcgttg acgctcttga agtgatccag
     5221 atccgccagc agaaccgccg cagacccctg ttcgcgcgcc cctcgcgcca actcgcgccc
     5281 cagcatgtcg aggatcgctt cccggttcag cagcgaggtc agcgagtcgt gggttgcttt
     5341 gaagtgcagg ttctcccggg cccgcttcag ctctgcctca gtctccttca gagcggtaat
     5401 gttgcgtccg atcccgataa tgcccaccgt tttcccttcc gggtcacgga acggcacctt
     5461 cgtggtcaac agccagcgaa tccgtccgtc gacctcccgg acatgttcgt cgtggctgac
     5521 caggggaatg cccgtctgga tgactttctg ctcgtcctga taaaagcccg ccgccaactc
     5581 gctggggtag tagtcaaagt cggacttacc acgcagctct tgtcccgact ctgcgcccat
     5641 cacgtcggcc gtgccctggt tggcgaataa aaaccggctt tccagatcct ttacgtaaat
     5701 aagatccggc acgctgccca tgacggcgcg cagcaactcc agttcgccgc cgaattgctc
     5761 cccgggtccg tgaaggcggc tcttcgcaaa acgccatgcc agaacaaagg caccggaaag
     5821 aaacaggaca agaaagaaaa gcaggaccca ccccagaaga ctcgcggggt agaacatcac
     5881 cgccgagctg cccaggttgc ccgtcaggcc cacgtggaat gcgaaagcgg gatcgatcgc
     5941 aggtttcatc gctgtccggt cgccgcgcgg cgagctgtgg ccgacgcgag accttcggac
     6001 gaacccgg
//
LOCUS       DASZYN010000643         1532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_735417, whole genome shotgun sequence.
ACCESSION   DASZYN010000643 DASZYN010000000
VERSION     DASZYN010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735417"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..>1532
                     /locus_tag="VHE33_16510"
     CDS             40..>1532
                     /locus_tag="VHE33_16510"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVT99109.1"
                     /translation="MRDKFLHIFLFCFSLSLLTLGLSGCGTTSGSRGGRGGGGGHGGG
                     GGNGGGGGPASLSSINHIVFLAQENRGLDHYFGALRQYWAQNGYPDQSFDGLPQFNPA
                     SGASPLQGAAPTNPGCDPTQPAPADCVVDTSNQVASFHLITQCAENPSPSWNEGHVDW
                     NYNDPTGQNAAALNGFVYTAAHDARTLTPPFTDTAGMRAMGYYTGDDLNYYYYMASKF
                     GTSDRWFNPIMTRTHPNRMYLIAGTSAGYAYPEGTDAQDSAKLSVKTIFDELQSAGIS
                     WKIYVDPTNSGCTQPYQASCLINLSYVNNFTFSNTLVSQYPNNIAPISQYFTDLQNGT
                     LPQVAQIEPPTDAGLDEHPTNNDAALSNIQSGAAWVATLINGLMQSTSWKDSAFILTY
                     DEGGGFYDHVAPQPAVSPDGIKPVDLLPGDICNGTTGPTCDFTYTGYRVPMIVVSPYA
                     KKNYVSHTVTDYTAILKLIETRFNVPSLTKRDAAQMDMTEFFDFNAPP"
BASE COUNT          325 a          437 c          477 g          293 t
ORIGIN      
        1 tccaagctgg ctgacttcaa caggagtctg gagagaccgt tgcgcgacaa gtttttgcac
       61 atttttctat tttgtttctc gctgtcgttg ttgactcttg gactgagcgg ttgcggaacg
      121 acaagcggaa gcagaggtgg ccgtggtggc ggtgggggcc atggcggggg tgggggaaat
      181 ggtgggggcg gtggaccggc gagcctgtcc tcgatcaacc acatcgtttt tctggcgcag
      241 gagaaccgag ggctcgatca ctatttcgga gccctgcggc agtattgggc gcagaatgga
      301 tacccggatc agtccttcga cgggttgccg caattcaatc cggcgtcggg cgcgtcgccc
      361 ctgcagggag cggcgccgac gaatccagga tgcgatccaa cccagccagc tccggcagac
      421 tgtgtagtcg ataccagcaa ccaggtggcg tcgtttcatc tgatcacgca atgcgcggag
      481 aacccgagcc cttcgtggaa cgagggccac gtggactgga actacaacga tccgacggga
      541 cagaacgcag cggcgctgaa cggtttcgtt tacacggccg cgcacgatgc gcggacgctg
      601 acaccaccct tcaccgacac ggcgggcatg cgtgcgatgg ggtactacac gggcgacgac
      661 ctgaactact actactacat ggcgtcgaag ttcgggacct cggatcgctg gtttaatccg
      721 atcatgacgc gcacgcaccc caaccggatg tatctgatcg caggaacatc ggcgggctac
      781 gcctatcccg aaggaaccga cgcgcaggat tctgcgaagc tgtcggtgaa gacgattttt
      841 gacgagttac agagtgcggg catcagttgg aaaatctacg tcgatccgac caacagcggg
      901 tgcacgcagc cctatcaggc gtcttgcctg atcaatctga gctacgtgaa caactttacg
      961 ttctccaata cgctggtctc ccagtatccg aacaacatcg cgccgatctc gcagtacttt
     1021 accgatctgc agaacgggac gctgccgcag gtggcgcaga tcgagcctcc gaccgatgcc
     1081 gggctcgacg agcacccgac aaataacgat gctgctctga gcaatattca gagcggcgca
     1141 gcctgggtcg cgacgctgat caatgggctg atgcagagca cctcctggaa agactcggcg
     1201 ttcatcctga cctacgacga ggggggtgga ttctacgatc atgttgcgcc gcagccggcg
     1261 gtcagcccgg acggaatcaa gccagtggac ctgctgccgg gggacatttg taacggcacg
     1321 acgggtccga cctgcgactt cacgtacacc ggctaccgcg tgccgatgat cgtggtgtcg
     1381 ccgtatgcga agaagaacta tgtgtcgcac acggtgaccg attacacggc gatcctgaag
     1441 ctgatcgaaa ccaggttcaa tgtgccgtcg ctgacgaagc gggatgcggc gcagatggac
     1501 atgaccgagt tcttcgactt caatgcgccg cc
//
LOCUS       DASZYN010000644         8115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_863476, whole genome shotgun sequence.
ACCESSION   DASZYN010000644 DASZYN010000000
VERSION     DASZYN010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8115
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863476"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1610)
                     /locus_tag="VHE33_16515"
     CDS             complement(<1..1610)
                     /locus_tag="VHE33_16515"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HVT99110.1"
                     /translation="MDVHGQALVLAAPCVLLFGALLQLAVAPLRSARIKGILAAASSL
                     PAALLVLLLIPSIHAGQVIDFNLFSWDGPLMLAVHVDGLGLLFALMGTGLGTIVLLYS
                     VAYMEHDRAATRFYAAMLVFIGGFVALVWSANLILLYLFWEVIGLCSFSLVGFWYTDR
                     EAVAGARKVLLMTHLAGYGLLAAILILYHRAGTVLWTDPAVTRNFTAVVFVLMLIAVV
                     AKSVQVPLHTWIPDAMAAPTPVSSLLHAACYVTAGVYLAARMHSFGIWPHLWGSSLVW
                     IGCVTMVVGVMYAMVQTDLKRMLAFSTVSQIGYMIMALGIGTSLAIIAGLLHCLNHGF
                     FKGGLFLNAGAVQHATGTRDMNRLGGLAQRMPRTTLAWLVGVGSMAGIPLLSGFVSKW
                     MLYAAALEAGWIVPALVAWIVSLGTVFLCAKATSSVFLGTPTADTENAHEAPLAMRWA
                     MGLMAAGNIVLGIAPQLAVNFLLLPVVSSFALSSSLQVTWFGLFTGTGSFSTTGGLVL
                     AVVSLVMGGVIYAFAYALRPAQVAVASGP"
     gene            2027..2341
                     /locus_tag="VHE33_16520"
     CDS             2027..2341
                     /locus_tag="VHE33_16520"
                     /inference="COORDINATES: protein motif:HMM:NF027832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrlR family regulatory protein"
                     /protein_id="HVT99111.1"
                     /translation="MEGLWHTRFNAGAAHGDGIVVLRNGEVLGGDASHIYVGSYQTDG
                     SRVYFNVRVSRWLEEDGTISPESPQAFLFSGSMEGNAAIVSGHPDDHREVTVAVELRR
                     AA"
     gene            2394..2933
                     /locus_tag="VHE33_16525"
     CDS             2394..2933
                     /locus_tag="VHE33_16525"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HVT99112.1"
                     /translation="MPILEEQALDAPERSAIDQIIAQHEGRSGALLGILAEAQSANSH
                     RFLSMPALRYIAENSGIPPARVYSAATFFALFNLQPQGDNVICVCRGTACHTRGSRDL
                     LNRLCIDLGFSAPDTTALSEADKLALTTADRRFTIRTVACFGQCALAPVVEVNHHILS
                     HVNERTLRREIESLGQRKK"
     gene            2933..6100
                     /locus_tag="VHE33_16530"
     CDS             2933..6100
                     /locus_tag="VHE33_16530"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HVT99113.1"
                     /translation="MSPILDIGAFNAVRESGLARLMPNIPRITVGMGTCGRGNGAEEL
                     FLALGEAINRSGQDVLLVGTGCFGSCFQEPMVGLRIPGFPLIQLRHVQARDAGRVLEA
                     VASRVMPPDLIFCKIEEWDHITTRLTYGQAWPEIPHWHEIPFFRGQKKIVLRNCGLIN
                     PEDIEEYIAVGGYQALYKVLIDARPESVIEQIKAARLRGRGGAGFLTGNKWDFLAKSK
                     SETKYIICNADEGDPGAYMNRNEIESDPHSLLEGMIIGAYVMGAAHGIIYVRAEYPLA
                     VHRLTRAIEQAREYGLLGHNILDRRFNFDIELVQGAGAFVCGEETALIASLEGSSGRP
                     HARPPYPAQKGLWGQPTNINNVETWCNVAPIVSRGPAWFAENGSSKSPGTKVFSLVGK
                     VQNTGLVEMPLGTPLRTFIYDIGEGAVNGRKVKAVQTGGPSGGCIPVEMLDTPVDYES
                     LAQIGSIMGSGGMVVMDEDNCMVDVARYFIEFTHSESCGRCVPCRIGLDKALRILNSI
                     SEGNGTHHQLNLLDELGRMIRESSLCGLGQSAPNPVLTTIRHFREEYEDHIVARRCRA
                     GVCQELALSPCENSCPLRMNIPRFLDLCHQGRLDDAFESVIFDNPLPASTGRVCQHPC
                     ENRCRRQAFDQVVNMREVHRFIADSVFQSDRFEPLAARIAARRFPPTGKKIAVAGSGP
                     AGLTAAFYLALLGHDVTVFEEREEAGGMLRYAIPEYRLPKAVLRREVELIQRVGARMV
                     FNTRIGVDITLNDLAAQFDAVFLSIGTWKESWLYLPGTELKGVYPALNFLESVAREQS
                     IIPGSRVCVIGGGNAAIDSARTVLRMGADVSILYRRERKDMPAIDEEVQAAEDEGVRF
                     VFLAAPHRILGDAEGNVKAIEVVKTRLGEYDKSGRRKPVLTDEVQRLRCDSVILAVGE
                     TFDLDFCRASGLALNEDGTIIVDRFTLETSRPGFYAGGDVITGASNVSNAMGGGKQAA
                     RVMDQRLMGEQRWDRLFPAFEYSHEPSRETSLSPRHAAPVLPVAMRVHSEDEVVARLD
                     TASAREESARCLRCDLRVTAGAEAP"
     gene            6112..6834
                     /locus_tag="VHE33_16535"
     CDS             6112..6834
                     /locus_tag="VHE33_16535"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HVT99114.1"
                     /translation="MPKKTISARIDGELITAREGQTILEAATAHGRHIPTLCWFKGLS
                     AVGSCRVCMVELEATDRLVAACTTPLQDGMSIRTNSPRLTLYRKMAVELLLVERNHVC
                     SVCVSNGHCEFQNLMISLGITHSRFSTDSPGYRVDMSYPRFVLDQNRCILCTRCVRVC
                     AEIEGAAVWEVAARGISSHIVSDLNDDWAHAKNCTGCGKCVQVCPTGALTEREVAVIQ
                     PLKRSENISRLAGKVARRDSSV"
     gene            complement(6942..7856)
                     /locus_tag="VHE33_16540"
     CDS             complement(6942..7856)
                     /locus_tag="VHE33_16540"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HVT99115.1"
                     /translation="MNQSQIDGLNRRFGIPNMVTVVSGNGGLPKIRVTAPWASAEIYL
                     HGAHLTSWKPMNFEEAIFLSAHSNWQDGLAIRGGVPICFPWFRAKADDPSAPVHGVVR
                     TKEWQLESVQIEGESVVVMCSAEDDESTHRWWPHAFRVVHKVSIGKSLRLELNVKNTG
                     QSPFRFEEALHTYFQVGNAAKVRIRGLDQSSYLDNVDGNHRKNQSGDLIFTGRTDNAY
                     IDAYGPVELIDATWRRRLLTEKVNSANTVVWNPGPGWAALPADLGKDEWQQFACVEAS
                     NIFDSAISLAPDQEHKLEAIIRVVPEDE"
BASE COUNT         1659 a         2650 c         2317 g         1489 t
ORIGIN      
        1 gggccggagg ccacagcaac ctgcgcaggc cgcagggcgt aggcgaatgc gtagatcacg
       61 ccgcccatca ccagcgaaac gaccgcgagc accagaccgc ccgtcgtcga gaagctgccg
      121 gtaccggtga acaggccaaa ccaggtcacc tgcaggctcg agctcagtgc gaaggagctg
      181 acaacaggca gcagcaggaa attgacagca agttgcggcg cgatgcccag cacaatgttt
      241 cccgcagcca tcaggcccat cgcccatcgc atggcgaggg gagcctcgtg cgcgttctca
      301 gtgtccgccg tcggcgtgcc gagaaagacc gagctggtgg ccttcgcaca aagaaaaact
      361 gtaccgaggc tcacgatcca cgcgaccagg gcaggaacaa tccatcccgc ttcgagtgcg
      421 gccgcataaa gcatccactt gctcacgaat ccgctgagca gaggaatccc ggccatgctc
      481 cccacgccga cgagccaggc cagcgtcgtt cgtggcatac gctgcgccaa tccgccgagc
      541 cgattcatat cgcgggttcc cgtggcgtgc tgaactgcac cggcgttcag gaagagtccg
      601 cctttgaaga aaccgtggtt cagacaatgc agcaacccgg cgatgatggc gagcgacgtg
      661 ccgatgccca gggccatgat catgtaaccg atctggctca cggtggagaa ggcgagcatg
      721 cgcttcaggt ccgtctgcac catggcgtac atcacgccca ccaccatcgt tacgcagccg
      781 atccacacca gcgaagagcc ccacagatgc ggccatattc caaagctgtg catgcgcgcg
      841 gccaggtaca cacccgccgt gacgtaacaa gcggcatgca gcagggaact gactggtgtc
      901 ggcgcggcca tcgcgtccgg aatccaggtg tgcaacggaa cctgcaccga ctttgcaacc
      961 accgcgatca gcatgagtac gaaaacaaca gcggtgaagt tgcgcgttac ggcgggatcg
     1021 gtccacagta ccgttcccgc gcggtgatac aggataagga tggctgccaa taatccgtag
     1081 cccgcaagat gggtcatcag cagaaccttg cgtgctccgg ccacggcttc ccggtcggtg
     1141 taccagaagc ccaccaggct gaaggagcaa aggccgatca cttcccagaa gagatacaga
     1201 aggatgaggt tcgcgctcca gaccagcgcg acaaagccgc caataaagac gagcatcgca
     1261 gcgtaaaagc gcgtggccgc acggtcgtgt tccatatagg cgacggagta aagcaggacg
     1321 atcgtgccca gcccggttcc catcaaagca aaaagcaaac caaggccgtc aacatgtaca
     1381 gccagcatca gcggcccgtc ccaggagaag agattgaaat cgatcacctg cccggcgtga
     1441 atggaaggaa tcagcagaag cacgagcagg gctgcaggca ggctgctagc cgcggcgagg
     1501 attcccttga tgcgtgctga ccgaagaggt gcgactgcca gctgcagcag cgcgccgaac
     1561 aggagcacgc acggtgcggc aaggacgagt gcctgaccgt gaacgtccac cagcgtcacc
     1621 tcccgctgtc acagccgcgc gcctcgctcg tttgcgggct ggatcacgct gcgacatcgg
     1681 ccggaaaggc gtggacagtt gagctgaaag aacgggctcc cgatgaactc tgtgcgcgcc
     1741 gcggcacaac gccgagcacg gcatcgtgat tagcacgttg catatgcgat gcgctgtgac
     1801 tggcatcact gcaaaacaca tctgcctctg cttaaacatg gcgaggcagt ttctgcacga
     1861 ttcacggcag tcgtgaacag gttctccctc gatgctgaag aaccatcggc tgcaatgctc
     1921 ctcgcgctct cgtgcttcgc gcagatgaag cagatgtatc tttccgagta atccctggga
     1981 acacgctgtt tcccggaaag ctgcttcgca gtgaggaggt agcggaatgg aaggcctttg
     2041 gcacacgcgg ttcaacgcag gtgccgcaca cggcgatggc atcgtcgtgc tgcgcaatgg
     2101 agaggtgctg ggcggagatg cctcgcacat ttacgtgggc tcgtatcaga ctgatggctc
     2161 acgggtatat ttcaacgttc gcgtcagccg ctggctcgag gaggatggga ccatctctcc
     2221 cgaatcgccc caggcctttc tcttcagcgg atcgatggag ggcaacgcgg ccattgtttc
     2281 aggccatccc gacgaccacc gcgaggtgac cgtcgccgtg gaactgcgtc gcgccgcgta
     2341 agtcgtgcag tcgcctgcgc tgcggcgctc agactgccag caaaggagcg atcatgccga
     2401 ttctcgagga gcaggcgctg gatgcacccg agcgaagcgc catcgaccag atcatcgcgc
     2461 agcatgaagg ccgatccggc gcccttctcg gaattctggc cgaggcgcag tcggccaatt
     2521 cgcacagatt tctctccatg cctgcgctgc gctatatcgc ggaaaactcc gggattcccc
     2581 cggcacgcgt gtacagcgca gccacgtttt ttgccctctt taatctccag ccgcaggggg
     2641 ataacgtgat ctgcgtttgt cgcggaaccg catgccatac ccgcggctcc cgcgacctgc
     2701 tcaaccgcct gtgtatcgac ctcggcttca gcgcgcccga cacaacggct ctcagcgagg
     2761 ccgataagct cgcgctcacc actgcggacc gcaggttcac cattcgcacc gtggcctgct
     2821 tcgggcagtg tgcgctggca cccgtcgtgg aggtgaacca tcacatcctg agccacgtga
     2881 atgagcgtac gctgcgccgc gaaatcgaat ccctcgggca gaggaaaaaa taatgtcacc
     2941 catcctcgat atcggggcgt tcaacgcggt gcgcgaatca ggactcgccc ggctcatgcc
     3001 gaacattcca cgcatcactg tcggcatggg cacctgcggc cgcggcaatg gcgctgaaga
     3061 actcttcctg gctctcggcg aagccatcaa tcgcagcggc caggatgtgc tgctcgtcgg
     3121 cacaggctgc ttcggctcct gctttcagga gcccatggtg ggcctccgca ttcccggctt
     3181 cccgctcatc cagctgcgcc acgtgcaggc gcgcgatgcg ggccgcgttc tcgaggccgt
     3241 cgccagtcgc gtcatgccgc ctgacctcat cttttgcaag atcgaagagt gggaccacat
     3301 caccacccgg ctgacctacg gacaggcctg gcccgagatt ccgcactggc acgagatccc
     3361 gttcttccgc ggtcagaaaa aaatcgtcct ccgcaactgt ggcctgatta accccgagga
     3421 cattgaagag tacatcgcgg tcggcggcta tcaggccctc tataaagttc tgatcgacgc
     3481 tcgccccgag tccgttatcg agcagatcaa agcagcgcgc ctgcgtggcc gcgggggagc
     3541 aggatttctc accggcaata agtgggattt cctcgccaaa agcaagtccg aaaccaaata
     3601 catcatttgc aatgccgacg aaggcgatcc cggcgcttac atgaatcgca acgagatcga
     3661 aagcgatccg cattcgctgc tcgaaggcat gatcatcggc gcttatgtca tgggcgccgc
     3721 gcacggcatc atctacgtgc gcgccgagta cccacttgcc gtgcatcgtc tcacccgtgc
     3781 catcgagcag gctcgtgaat atggactgct cggccacaat atcctcgacc ggcgctttaa
     3841 tttcgatatt gaactcgtcc agggagcagg agccttcgtc tgcggcgaag aaaccgcatt
     3901 gatcgcctcg ctcgaaggca gctccggacg tccgcacgcc cgcccgccct acccggctca
     3961 aaaaggtctc tggggtcagc cgaccaatat taataatgtt gagacctggt gcaacgtcgc
     4021 gcctatcgtc agccgcggcc ccgcatggtt cgcagaaaac ggcagcagca agagccccgg
     4081 caccaaggtc ttctcgctcg tcggcaaagt gcagaacacc ggcctcgtcg aaatgcctct
     4141 tggaaccccg ctccgcactt tcatctacga cattggcgaa ggcgcagtca acggccgcaa
     4201 agtgaaagcc gtgcagaccg gcggcccctc gggcggatgc attccggtcg agatgctcga
     4261 cacgcccgtc gactacgaat ccctcgcgca gatcggctcc atcatgggtt cgggtggcat
     4321 ggttgtgatg gatgaagaca actgcatggt cgatgtcgcg cgctacttca tcgagttcac
     4381 ccactcggaa tcctgcgggc gctgcgttcc gtgccgcatc ggtctcgaca aagcactccg
     4441 catcctgaat tccatcagcg aaggcaacgg aacccatcac cagctcaatc tcctcgacga
     4501 actcggccgc atgatccgcg aatcctctct ctgcgggctc ggccagagcg cgccgaaccc
     4561 ggtcctcacc accatccgcc acttccgcga ggagtacgag gaccacatcg ttgcccgccg
     4621 ctgccgcgcc ggcgtttgcc aggaactcgc gctttccccc tgcgaaaaca gctgtccgct
     4681 gcgcatgaac atcccgcgct ttctcgatct gtgccaccag ggccgccttg acgatgcttt
     4741 cgaatcggtc atcttcgaca accctctccc cgcatcgacc gggcgcgttt gccagcatcc
     4801 ctgcgagaac cgctgccgcc gccaggcctt cgatcaggtc gtcaacatgc gcgaggtgca
     4861 tcggttcatt gcagactccg tcttccagtc cgatcgcttc gaaccgctgg ccgcgcgcat
     4921 tgcagcccgc cgcttcccgc ccaccggaaa gaaaattgcc gtagccggtt ccggccctgc
     4981 aggcctcacg gctgccttct accttgcgct gctcggccac gacgtcaccg tctttgagga
     5041 gcgtgaagaa gccggcggca tgctgcgtta cgccatcccc gaatatcgcc tgcccaaagc
     5101 cgtgctgcgc cgcgaggtcg aactcatcca gcgagtcgga gcaaggatgg tgtttaacac
     5161 tcgcattggc gttgacatta ccctgaacga tcttgctgcc cagttcgacg cggtcttcct
     5221 ctccatcgga acgtggaagg aatcctggct ctaccttccc ggcaccgaac tcaaaggtgt
     5281 ctaccccgcg ctgaactttc tcgagtccgt cgcaagagaa caatccatca tccccggctc
     5341 ccgcgtatgc gtgatcggcg gaggcaatgc cgccatcgac tcggcgcgca cggttcttcg
     5401 catgggcgcc gatgtctcca tcctctatcg gcgcgagcgc aaggacatgc cggcgatcga
     5461 cgaagaagtc caggcggctg aggacgaagg cgtgcgcttt gtcttcctcg ccgcgccgca
     5521 tcgcatcctg ggggatgccg aaggcaatgt taaagccatc gaagtggtca agactcgtct
     5581 gggcgaatat gacaagtcgg gccgccgcaa gcccgttctc accgatgaag tccagcgcct
     5641 ccgctgcgac agcgtgatcc tcgccgtcgg cgaaaccttc gacctcgact tctgccgcgc
     5701 ctccggcctg gctttgaacg aagacggcac catcatcgtc gatcgcttca cgctcgaaac
     5761 cagccgcccc ggcttctacg caggcggaga cgtcatcacc ggcgcctcga acgtgtcgaa
     5821 tgccatgggg ggagggaagc aggccgcccg cgtgatggac caacgcctca tgggcgagca
     5881 gcgctgggat cgcctgttcc ccgccttcga atacagccac gaaccgtcgc gggaaaccag
     5941 cctcagtccg cgccacgcag cgccggtgct gcccgtggcg atgcgcgttc actccgagga
     6001 tgaagtggtg gcgcgcctcg atacagcctc ggcacgcgag gaatccgcgc gctgcctgcg
     6061 ctgcgatctg cgcgtcaccg cgggagccga agcaccgtga aggagaccaa cgtgccaaag
     6121 aagacgattt ccgcgcgcat cgacggtgag ctgatcaccg cgcgcgaagg ccagacgatt
     6181 cttgaagccg ccaccgcgca cggaaggcac attcctactc tgtgctggtt caaaggcctc
     6241 agcgctgtcg gttcctgccg cgtctgcatg gtcgagcttg aggccaccga ccgtctggtc
     6301 gctgcctgca ccacgcccct ccaggacggc atgtcgatcc gaacgaattc tccccgcctc
     6361 acgctctacc gcaaaatggc cgtcgagctc ttgctcgtcg agcgcaatca cgtgtgttcg
     6421 gtctgcgtct ccaacggtca ctgcgaattc caaaacctga tgatctctct cggcatcacc
     6481 cactcccgct tctcaaccga cagtcccggc taccgcgtcg acatgagcta tccccgtttc
     6541 gtactcgatc agaaccgctg catcctttgc actcgatgcg tgcgggtgtg cgcggagatc
     6601 gaaggcgccg ctgtatggga ggttgccgca cgcggaatct cctcgcacat tgtcagcgat
     6661 ctgaatgacg actgggctca cgcgaaaaac tgcaccggct gcggcaaatg cgtccaggtg
     6721 tgtccaacag gcgccctgac cgagcgtgaa gtcgccgtca tccaaccgct gaagcgaagc
     6781 gaaaatatct ccagacttgc cggcaaggtc gcgcgccgcg actcatccgt gtgatccagt
     6841 tgcgagtcct gaaggcgaac gccgcatcgt gaccgttgag gttttggatt ctcacatcga
     6901 tcgccgcagc cgcgcgcaaa gtatcgccag gtacgaaatg ctcattcgtc ttcaggaacg
     6961 acacggatga tggcctccag cttgtgttcc tggtctggag cgagcgagat cgccgagtcg
     7021 aaaatgttac tggcctccac gcaggcgaat tgttgccact catcctttcc cagatcggca
     7081 ggcaaagccg cccagcccgg tcccgggttc cagaccacag tgttcgccga gttcactttc
     7141 tcggtcagta accttcgacg ccatgtcgca tcgattagct ccacagggcc ataagcgtcg
     7201 atgtaggcgt tgtctgtccg ccctgtaaag atcaggtctc cggattggtt cttccgatgg
     7261 ttgccatcga cattatccag ataagagctc tgatcgagac cgcgaatgcg cacctttgcc
     7321 gcgttgccca cctggaagta tgtgtgcagc gcctcctcaa atcgaaaagg gctttgcccc
     7381 gtattcttca cgttgagctc aagccgaaga gatttgccaa tggatacttt gtgcaccacg
     7441 cgaaaggcgt gcggccacca tcgatgcgtc gattcatcat cctccgcaga gcacatgaca
     7501 acaacggatt ctccttcgat ttgcaccgac tcaagctgcc attccttggt gcgaaccacg
     7561 ccgtggacgg gggccgatgg atcgtcggct ttggctcgaa accatgggaa gcagatcgga
     7621 actcccccgc ggatcgcgag gccgtcctgc caattggaat gcgcgctgag gaaaatcgcc
     7681 tcctcgaagt tcatcggctt ccatgacgtc aggtgggcgc catgaagata gatctccgcc
     7741 gaagcccagg gagcggtcac ccgtattttg ggcaacccgc cgtttccgga gaccactgta
     7801 accatattcg gaataccaaa tctgcggttc agcccgtcga tctgtgattg gttcatttga
     7861 tacaagagtc tatcgaaagt cgtgtggcgt cacctggcct cccgattgag agaacaccat
     7921 gccccttcgg cacatcgccg gtcgtaaaat gaaaagactc gccgtgaacc gcgccgccca
     7981 tcttcttctc gcttttacgt tcgccacact tccagctgtg tcagtccacg ctgccgcggc
     8041 gaacggctcg cggccaaagc gcctgatcgc ggccctcctg gccgaaatcc cggacacccc
     8101 ttcggctctc gcggt
//
LOCUS       DASZYN010000645        17443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_355929, whole genome shotgun sequence.
ACCESSION   DASZYN010000645 DASZYN010000000
VERSION     DASZYN010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 17443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17443
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355929"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..4074)
                     /locus_tag="VHE33_16545"
     CDS             complement(109..4074)
                     /locus_tag="VHE33_16545"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HVT99116.1"
                     /translation="MKMHTLPAWRECFSRAIFAASLALSLCLIPHLQAQTAGEGSIQG
                     TVTDSSGAVVRQATVSAVSSATGIATTSTSTSSGLYVISPLLPGNYTVTVTASGFRQF
                     RQQNLVVVSMNATTLNVSLQVGAETQQITVTAAPPALDTTDATLGATISSDVYLDLPI
                     LANNQQRDITAMSNLLPGAQPGSRSSLFSGTASRVEEVYLDGIPLTTISQIGDNRPIL
                     NIVPAEAIDQINVVTNGASAEYQGAGMVNYTTKSGGNKYHGTVADYVRNTIFDTWGFT
                     AIANTKKELVNGAITSVPAGKPMDHQNEFTVSVGGPVSIPLLFSGKDKLFFFASYDKF
                     HSRAAPNPSQVTIPTTAFRTGDFSALLASNGGPGYAIYDPTTLASCTAHSTNGPCRYQ
                     FGYGPGAAPGPGGNPTPTGAPVNVIPASEISPIAQYMQKFLPDPTTSAISGNYLAGPP
                     SGYDNWLYSARIDYDVSQKQRISGIVTGGNRQAYPFTAAGSYNNSSPALPMPYTGSDY
                     SIVAGHWIDLEDAYTFTPNLVNQFRFGWSNFGGPPLKNLTEGIKDYEATSTGMTFSGV
                     PANGQAVTEFPTIQFNGSNSPNTWGFGNDGVTATTVSQNYTALDNLMWVRGKHAMTFG
                     FQMQRLEENASVYDGPTSALTMAFSTPETASVVPSGSSYAYGGNTGYSYASFLLGAVN
                     GTGITLQPFSVLGGRYHTYAPYFQDEWKVTSKLTLDLGLRWDYLPPYHEVLDRYSFLN
                     PNITNPVTGNKGALQFAGSWGGSSVSCNCVSPVHTYWKNWQPRVGIAYSLNDKTVISA
                     GFATVYSHAGGTGGAGGTYSGTGQLGFTSSPSFPDNAAGPDAGPSFYLNNSDAFKAMG
                     LDNTTFGGPGYTVPAITAPGSVSQTLNVGNTVGSDGKFITAGSAPGYPDPYLSGRAPQ
                     FNFWNLSVQRAVTNDITIMAAYAGTESHFIAGASNMRGKYVNQINPAYWSLGSLLNSP
                     ATPTNVAKAQAIIPGIQPPYSSYEDAAVINGGQATIGHMLTWMPQFSSVSDTWGTSSA
                     NASYHSLQIQLRKRMSHGLDLTVNYTYSKNIDDAGTQRSGFAIPAAYTLTGRSWPQNR
                     IDRSISANSIPQDLNAYGTYHLPFGRQGWGSGNWFTRSLIRGWNLSGLFSYHSGTPLL
                     VTSSACNGSTHPGAGTCMPDLNPAFSAKTTRQNGSWGKGITAKTFGTIHYLQGYVPST
                     ADATGVAGDGKTAVPCGSSSGPYCNAGSFMFGDAPRVLAYGGLRNPSTYDLSGSVSRT
                     FDITERWKFILRADCQNIPNKVTFGGIQANMNNAAFGTVGSATGNTGSRDFQLSGRIT
                     F"
     gene            4288..5592
                     /locus_tag="VHE33_16550"
     CDS             4288..5592
                     /locus_tag="VHE33_16550"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HVT99117.1"
                     /translation="MAWAVQGKAILLARMMILLPWLGALAQPAGWKLVCTDTPRHGET
                     SIIGARYERALHDFGFDLAPSPPSIHGTCVAGTPFYVSVQEPDGDYRVTVLLGAEQSA
                     SVTSVKAEARRLMIWNAPTPPGQTIQRSFVVHVRRPAVNASENVRLKPREVGNLDWDE
                     KLTLEFTGDHPSLRRIEIQPVRVPTVYLAGDSTVVDQDDEPWAAWGQMLPAFFNPKIA
                     VANEAESGETIASFAAEHRFDKIFSTLRSGDYLLMQFAHNDQKPGRGFVSISEYKDLL
                     RRYIAMTRKRKAHPILVTSMNRRRFSAEGRIVATLGEYPDAMREVAREEKVPLIDLNA
                     MSRTLFEAMGPDGTLKAFVHYPANTFPGQIEELKDDTHFNSYGALELAKCVVEAIREQ
                     NLPLANFLNPDPQRFDPAHPDPPSIWELPTDPFWSQQKPYER"
     gene            5653..7008
                     /locus_tag="VHE33_16555"
     CDS             5653..7008
                     /locus_tag="VHE33_16555"
                     /inference="COORDINATES: protein motif:HMM:NF025934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HVT99118.1"
                     /translation="MKNSFGKNRGLCVSLLALLLPVMAHAQKPASTVAPGAVGSFPSW
                     QPGQEPPTSWVDASTGHRVVRLTREPDSASFYFNVNAYTPDGKEMVYTTPDGISVLDL
                     TTWKTRSVVEGHVRTIVVGHKTPSVFYIKPEERSLYVTNVDTGETRKLAIMPARGSVA
                     TVNADETLAAGTYIEGTGGPDYNQPPPGDGHAQDQRGGSQQTHNMLQPLNKGQMMEQR
                     LAARLPLVLFVIDLRTGSLRPLLHSTDWINHLLFSPTDPNLLMYCHEGPWQKVDRIWT
                     IHTDGTHNTLIHKRTMFMEIFGHEFWAPDGKTVWYDWQTPKGEDFWLAGYNVETGKRT
                     AYHMQRNEWSIHFNVTRDGSLFCGDGGDPGQVAHALDGEWIELFHPEMIKGQGINEPG
                     FWQPGVFHSEHLVNMSRHNYRLEPNVSFTPDQKWVLFRSNMFGPTYVFAAEVAKAKTG
                     D"
     gene            complement(7036..7662)
                     /locus_tag="VHE33_16560"
     CDS             complement(7036..7662)
                     /locus_tag="VHE33_16560"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HVT99119.1"
                     /translation="MNQELFTLSGRHVRLEPLEARHVEPLAAAASNAGDLYRWTVVPQ
                     GVAKMTKYVDTAIGWRQEGVAVPFATVRQADGAVIGSTRFFDIERFPWPENHERHGRL
                     HPDVCEIGYTWLEPSSIRTPANTEAKLLMLTHAFEGWGVLRVCLHTDERNERSRAAIE
                     RIGARFEGILRAHRMASDFIARNSARYSIVASEWPEAKRRLAARLENR"
     gene            complement(7659..9929)
                     /locus_tag="VHE33_16565"
     CDS             complement(7659..9929)
                     /locus_tag="VHE33_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99120.1"
                     /translation="MNVSRFAACLFLFTAVTGPAQRTPSEHYTWKNVQIVGGGFVDGI
                     IFHPSAPGVRYARTDIGGAYRWNQASHRWQPLLDWIGERDHNLMGVESIAIDPSDPDR
                     LYMACGTYTNSSAPDGAILRSADRGRTFQRADVPIKFGANEDGRGNGERLAVDPRDGR
                     ILFLGTRHDGLWRSHDRGASWSRVASFPDITEAAPAHGDGIVFIHFVSKPGHAHKATE
                     TIYAGVSLMNRPTLFVSKDAGATWRPVANAPAQYRPTRSSLSSDGFLYISYGTAPGPS
                     RMTDGAVWKLNTHTGAWTDVTPEHPVRGSKEFGYAAVAVDAHHPQNVIASTFGRPGFD
                     GGETIFRSTDAGATWKPLFGSVHSGIVDDSLAPYVHTTPIHWMFDVEIDPANSDHAVF
                     STGYGGLETFDLSAVDRGQSTHWSILVPGIEETVALQLDSPVNGASLISAIGDYGGFV
                     HWDLDRPAPEGSSAPPRFGNTTGIVSAPLRPEVLVRVGISAEHKPGENISYSADSGHT
                     WKPTSKAPSPASHAGSIAVSADGATWVWTPQGETPSLTHDRGATWKTAQGLPTGTRVI
                     ADPKDSDIFYAASLLDHTLYRSTDAAARFAAQHFTLQSAPPASPAPRGDPRGGQDHLY
                     AAPGRSGDLWFAAFDGLYHANLTDQTGEEISFQRIPSATEIEAFGFGKAAPGHTYPTI
                     YLVGTTEGQYGIFRSIDEARTWTRINDHQHQWGLILQITGDPRVYGRVYVGTHGRGIL
                     YGDPSGRTEHTGRTRR"
     gene            complement(10018..11508)
                     /locus_tag="VHE33_16570"
     CDS             complement(10018..11508)
                     /locus_tag="VHE33_16570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99121.1"
                     /translation="MKRDELVTIFACANVRVASLLVPRANRAAWRAEWDAEIYHLRRA
                     SAAELSDSPGTTPLEFSRGAFPDAWWLCRDHLRSHGASAWQAGSAGRCVATLTALMGA
                     ALILCMILPGARRSLLPLFPSQAADLVTISPAGYAGAESPGISLQQYQEWQTNTQKLF
                     SELAWYHPTVKNVVLAHESTARLSIGLASANFFRLLRVQVPDQASGGAGPTLVLTRSA
                     WRTLYRSDPRIVGQSATIDGKSVLVAVLHDDSPRLPGAMQGWLFQDHAALRQLSPHAR
                     GFAFARIRDAAFPPARSGVRIMLETRWGITHVFDCVSISWLTWQPTSNFLFALLLACL
                     ALPATTALPLGDYPRYRGRMVFRITVRRWSFLVLKLLLLVAAVYLATIALGRGPAWAG
                     STSVFLQVGAAFPALLFGFRWALQDQRRRCPECLRQLSSPARVGQASCNFLSWNGTEL
                     FCIRGHGLLHIPELPTSWFSTQRWLCLDSSWRCLFPENCAPSPEMI"
     gene            complement(11505..11822)
                     /locus_tag="VHE33_16575"
     CDS             complement(11505..11822)
                     /locus_tag="VHE33_16575"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVT99122.1"
                     /translation="MPADAKLSHTAAMILQAIHAGHIYGFSVMQMTGLPSGTVYPAMR
                     RLERDGLIVSKWEQQSIADTEQRPPRKYYRLTRTGRTTLEAAWVRYPLLEKMIPTPVM
                     ERA"
     gene            11900..12673
                     /locus_tag="VHE33_16580"
     CDS             11900..12673
                     /locus_tag="VHE33_16580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT99123.1"
                     /translation="MLELQHLSRSYRGIPAIEDVSFRVLAGEIVGFLGPNGAGKSTTV
                     KIITGLIRPNDGRVLFEGKDIHTDLINWRASFGYIPEEAHLYAWMSAMEYLQLVGRLR
                     GMRERVIEEKATAMLALLGLESWRHSPMTVYSKGMRQRVLMAAALMHDPRMLVFDEPL
                     SGLDVLSARLFRDLLHELAAAGRAVLYISHVLEIVEQICDRVVVIAQGKVVADAAPSE
                     LTRLMELPSLERVFAQLVRQQDTRSAARDIVEVMRIGHG"
     gene            12666..14474
                     /locus_tag="VHE33_16585"
     CDS             12666..14474
                     /locus_tag="VHE33_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99124.1"
                     /translation="MASPARQAKPPRHEVLSLEVLARPESQSPEGSPGQFRVLVQHFL
                     DRFFNNELASGEGDAKTRLVQAACTIGIPGLVVAMYLYPVYHLPRGHVGRYWGPRPFW
                     SQVGDHYFYVVYSMVAMGLVTIFEWDLLFPDLLDVVVLSPLPTRSWKMLGARVAAIFI
                     LLGAALFDANFLAPLVLPAAVDPPHLVRFWAAHLLAVAASGGFGAAFFLALEGALLGL
                     LGDRLFRKISLWLQGLFVWGLLTLLFLYPAMAGTMSGLLAPDSPVAAWVPSFWFLGIY
                     QRILDGPSTPAIFVRLAHIAEVALIAAIAVAIASYPLAWWRKTRSLVEGTAKRETRNW
                     AVAPVQWLVHLAVARTPASRAIWQFIGQNLLRIPRYRMVLVMYGGMGLALLFATVTRV
                     ALQRGHLLFFFSRDGLRATVPIVAFWTVSGLHSTFLAPADQRGRWIFRAILGKAGIAE
                     LVAAKRWVLFWSALLSMTAAGCAYALAAGPRSWLFAADQLLVSLGWSLILTDLFFLNA
                     TSIPFTGARPNSATNFALLLIPYLGFFPAVVMFTVALEPVLEVSVGHLACGVGLVAVA
                     HLILHGMHRSRIADHVHGIEPDEDEEEFPLRLGLRY"
     gene            complement(14471..14974)
                     /locus_tag="VHE33_16590"
     CDS             complement(14471..14974)
                     /locus_tag="VHE33_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99125.1"
                     /translation="MDLKKFFLEQLESEAASSRKALERMPEGQNSWKPHERSMEFGYL
                     ASLVAGMPGWVAMMIDNDETDLNAGGASFRTHAVGTRAELLAMLDEGLRRSRNALENT
                     TEEHLMKPWRLKMGDQVLAEGPRYSQIRDGALSHLAHHRGQFTVYLRLNEAKVPAIYG
                     PSADEWH"
     gene            15075..16037
                     /locus_tag="VHE33_16595"
     CDS             15075..16037
                     /locus_tag="VHE33_16595"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HVT99126.1"
                     /translation="MKADRLLSALFLLQAHGRLPEREIAERLEVSQRTAHRDMEALCA
                     AGVPLIAHRGVRGGWELEKGWRTRVPGLDETELRALLMAQPSGLGDRRLTAAAERAFN
                     KLLASLSPPMRAQAESMRVRLHVDATGWRPTTEDLSALSVVQDALERDVKLTFQYAGV
                     KGTTTSRTVDPLGLVSKQTAWYMVARAPEGMRTFRVSRMRDAVALAIRFERPPRFNLA
                     VYWKRSTEALQTGRQKYKAILGLSSEGVTALAQWVAMKPLEGKKLDSLQGWQAFVVEF
                     DSFYSARFVALGLGGSAVVLGPEDLRRDVGEQASAVARATQELS"
     gene            complement(16044..>17443)
                     /locus_tag="VHE33_16600"
     CDS             complement(16044..>17443)
                     /locus_tag="VHE33_16600"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HVT99127.1"
                     /translation="WSTNCVEWVMMHMGTARAGASLVNVNPAYRSHELQYTLTRSRMK
                     ALFLWSHDKRADYAEILGRARHNLTLELRQTVFFDSPDWTALLEAPGQIPDRVDAQDI
                     ANIQYTSGTTGLPKGVMLSHHNIVNNGQFLAQGFHYTEHDRVCVPVPLFHCYGCVIGT
                     MSALNTGAALVFPNWTFDARATLQAVHSERCTSLYGVPAMYVAELALPEFAEFDLTSL
                     RTGMMSGAPCPIELMKRVLHDMHIPELVIAYGQTESSPVVTMSDADDLIEIRVNTVGR
                     AMPQTEIRIVSTSDKTTLPVGQQGEVCARGYAVMKGYDAEPDATRLSIDADGWLYTGD
                     LGVMHPDGCIHLTGRSRDIIIRGGENIYPREIEEFLYTHPKISEVQVVGIPNERLGEI
                     VVAWVRLAPNQTATEEEIRIFCRDQIAYFKIPEHIRFVTEFPATLSGKIQKYKMREFE
                     IEARGLQNAAAARTA"
BASE COUNT         3470 a         4949 c         5465 g         3559 t
ORIGIN      
        1 gcgccgattc cctggttgcc gttgcgacag ccccggcaaa cgttacaaaa tgatcgggac
       61 acaactcatc cgagttgtgg cctgagagca aagtttagag caggacggct aaaaggtgat
      121 ccgtcccgag agttggaaat cccgacttcc ggtgttgccg gttgcgctgc caacggtgcc
      181 gaaggccgca ttgttcatat tggcctgaat tccgccaaag gtgactttgt ttggaatgtt
      241 ctggcaatca gcccggagga taaacttcca gcgctcggta atgtcgaacg tgcgcgagac
      301 actgccgctg aggtcatagg tgctcgggtt tcgcagcccg ccataggcaa gaacacgggg
      361 agcgtcgccg aacatgaacg atccggcatt gcagtaaggc ccgctgctgc tgccgcatgg
      421 gacagcggtt ttgccgtcgc cggcaacgcc ggtggcgtcc gctgtactcg gaacgtaccc
      481 ctgcaggtaa tgaatcgtac caaaggtctt cgccgtgatg cccttgcccc aactgccgtt
      541 ttgacgggtg gtctttgcgg aaaaggcagg atttaaatcg ggcatgcatg tgcccgcgcc
      601 cggatgtgtg gaaccattgc aggcggacga tgtaacgagc agcggagtcc cggagtggta
      661 ggagaacagc ccggacaaat tccacccccg aatcagtgaa cgcgtaaacc aattccctga
      721 tccccagcct tgtctcccga agggcaggtg gtaagtgccg taggcattca ggtcctgtgg
      781 gatgctgttg gcgctgatgg aacgatcaat gcggttctgc ggccaggacc tgccggtgag
      841 ggtgtaggcc gcggggatgg caaagccact gcgttgcgtt cccgcgtcgt cgatgttttt
      901 ggagtaggta tagttcacgg tgaggtcaag tccgtgtgac attcgcttac gcaactgaat
      961 ctgcaacgaa tgatacgagg cattcgcgct cgaagtgccc catgtgtcgc tcacactgga
     1021 gaactgcggc atccaggtca gcatgtgacc tatggtcgcc tgaccaccat tgatgaccgc
     1081 ggcatcttca taactggaat aaggcggttg gatccctgga atgatcgcct gcgctttggc
     1141 tacattggtg ggggttgcag gtgagttgag cagcgagccc aacgaccagt acgcggggtt
     1201 aatctggttt acatacttgc cccgcatgtt gctggccccg gctatgaaat gactctctgt
     1261 gccggcataa gcagccatga tagtgatgtc gttggtaacg gcgcgctgaa ctgagaggtt
     1321 ccagaaatta aattgaggcg cgcggccgga aagatatgga tccggatatc cgggggcact
     1381 gccggcggtt atgaactttc catcgctccc tactgtgttg ccaacgttca gggtttggct
     1441 gactgagccg ggagccgtaa tcgcgggaac agtgtaaccg ggaccgccaa aagtggtgtt
     1501 atcgagtccc atcgctttga aagcatcgct gttgtttagg tagaaagaag gccctgcgtc
     1561 aggtcccgct gcgttatccg ggaagcttgg tgaactggtg aaacccaatt gtcctgtgcc
     1621 gctgtaggtc cctcctgcgc cgccggtgcc acccgcgtgc gaatagactg tagcgaaacc
     1681 ggcactgatt acggtcttat cgttgaggga ataggcgatg ccgactcgtg gctgccagtt
     1741 tttccaatac gtgtgtacag gcgacacaca gttacaggac acgctggaac cgccccatga
     1801 cccggcgaac tgcagagctc ctttgttgcc cgtgaccggg ttcgtgatat ttggattcag
     1861 gaacgagtac cggtcgagaa cctcatggta gggcggcagg tagtcccacc ggaggcccag
     1921 atcgagggtg agcttcgaag tgaccttcca ctcatcctga aaataggggg cgtaggtatg
     1981 atagcggccg cctagcacgg aaaagggttg aagcgtgata ccggtgccat tgacggcgcc
     2041 gagcaggaaa ctggcgtaag aatagccggt attgcctcca taggcatagc tggaaccgct
     2101 tggcacgacc gaagcggttt caggtgtact gaatgccatg gtcaaagccg aagtcgggcc
     2161 gtcataaaca gacgcgtttt cttccagtcg ctgcatctgg aagccaaagg tcatggcgtg
     2221 cttccctctg acccacatca gattgtcgag ggcggtatag ttttggctta ctgtcgtcgc
     2281 ggtcacgcca tcattgccaa atccccaggt attcggcgaa ttgctgccat tgaactgaat
     2341 cgtcgggaat tcggtaaccg cctgcccgtt ggcaggtact ccggagaaag tcatgcccgt
     2401 ggacgtggct tcataatctt tgattccctc ggtgagattt ttgagtggtg gtccaccgaa
     2461 attgctccat ccgaaacgga actggttaac cagattgggc gtgaacgtat aggcatcttc
     2521 gagatcgatc caatggccgg caacgattga ataatccgaa ccggtgtagg gcattggcaa
     2581 ggcggggctg ctgttgttgt atgatccggc agcggtaaag gggtacgcct ggcgattacc
     2641 accagtgaca atcccggaga tgcgctgctt ttgggagacg tcgtagtcga tgcgggcaga
     2701 atagagccag ttatcgtacc cggagggagg gccggcgaga tagttgccgc tgatcgccga
     2761 cgtggtggga tcgggcagga acttctgcat gtattgagcg atcggcgaga tttcgctcgc
     2821 cgggatcacg ttaacgggag cgccggtcgg ggtgggattt ccgcctgggc cgggtgcggc
     2881 gccagggcca tagccgaact gataccggca ggggccattc gtgctgtggg ctgtgcaact
     2941 ggcgagggta gtcggatcgt agatggcata gcccggaccc ccattggacg cgagcaacgc
     3001 ggagaagtcg ccagtgcgaa aggcggtggt tggaatcgtc acctggcttg gattgggagc
     3061 ggcgcgtgag tggaacttgt cgtacgaagc gaagaagaac agcttgtctt ttccgctgaa
     3121 gaggagagga atggagacag ggccgcccac cgatacagtg aactcatttt ggtgatccat
     3181 cggcttgccg gcgggaacgc tggtgatcgc gccgttcacg agttcctttt tcgtattggc
     3241 gatggccgta aatccccagg tgtcgaagat ggtgttgcgc acatagtcag ccaccgtgcc
     3301 gtgatacttg ttgcctccgg atttagtggt gtaattgacc attccggctc cctgatattc
     3361 ggcggaagcc ccatttgtga ctacgttgat ctggtcaatg gcttcagcag gaacaatatt
     3421 gaggatcggc cgattgtcac cgatctggct gatggtagtc aggggaatgc catcgagata
     3481 aacctcttcg acgcggctcg cggttcccga gaaaagcgag gaccgcgaac cgggttgtgc
     3541 tcccggcagc aaattggaca tggctgtgat gtcgcgctgc tgattgttcg ccaggatcgg
     3601 gagatcgaga tagacgtcac tgctgatggt ggcgccgagc gtggcatccg tggtgtcaag
     3661 tgccggggga gccgcggtca ccgtgatctg ctgggtttcg gcaccaacct ggagtgagac
     3721 attcaaagtc gtggcattca tcgaaacgac cacgaggttc tgctggcgga actgccggaa
     3781 tccggaagca gtcacagtga ccgtgtagtt tccaggaagc agcggcgaaa ttacgtaaag
     3841 gccgctcgaa gtgctggtgc tggttgtagc aattccggtt gcgctgctga cggcgctcac
     3901 cgtcgcttgc ctcacaaccg cacccgaaga atctgtgaca gtgccctgga tggaaccttc
     3961 gcccgcggtc tgcgcctgaa gatgaggaat cagacacagt gagagcgcca ggctcgcggc
     4021 aaaaatggcg cgactgaaac actcccgcca ggcaggcagg gtatgcatct tcatagagcc
     4081 tccaaaagac gacagggttg gggattgcag aactagtaca acttcgggac actaacggac
     4141 cttaacagcg tctgtcaata gaggatgtgt ttcaatatag aaaatgcgga cggacctctg
     4201 tcacagcaag agccagggtt cagtcaatcc gcgagagtca aatattcaga tcagagggtg
     4261 ttagcgttgc cgttcggatc tccgagagtg gcctgggccg tgcaaggaaa ggcgattctg
     4321 ctggcaagga tgatgatcct gttgccatgg ctgggtgccc ttgctcaacc ggcaggctgg
     4381 aagcttgtct gtacagacac tccgcgccac ggtgagacat cgatcatcgg ggcccgctat
     4441 gagagagcgc tgcatgattt cggatttgat cttgcaccgt caccaccatc gatccacggc
     4501 acatgcgttg cagggacgcc gttctatgtc tccgtacagg agccggacgg ggactatcgt
     4561 gtgacggtcc ttctgggggc agagcagtcc gcctccgtca cctcagtgaa agcggaggct
     4621 cggcgcctga tgatctggaa cgccccgacg ccgcccggtc agaccattca gcgcagcttc
     4681 gtcgtgcatg tccggaggcc ggctgttaac gcatcggaaa acgtgcgttt gaaacctcgc
     4741 gaagtcggca acctggactg ggatgaaaag ctgacactcg aattcaccgg agaccatcct
     4801 tcgttgcggc ggattgagat tcagcccgtt agagtgccaa ctgtctatct ggccggtgac
     4861 tctaccgttg ttgatcagga tgatgaacca tgggccgcgt ggggacaaat gttgcccgcc
     4921 ttcttcaacc cgaagatcgc tgtggcgaat gaagctgaat ccggggaaac cattgccagc
     4981 ttcgccgccg agcaccggtt cgacaagatc ttcagcactc ttcgatcggg tgactacctg
     5041 ctgatgcagt tcgcgcacaa tgaccagaaa ccgggtcgcg gtttcgtttc tatttccgag
     5101 tacaaggacc tgttgcgacg atacatcgcg atgacgcgga aacgaaaagc tcatccgatt
     5161 ctcgtgactt caatgaaccg gcgaaggttc agtgcagaag gcaggattgt ggcaacactc
     5221 ggtgaatatc cggacgcgat gcgtgaggtt gcacgtgagg agaaggttcc cctgatcgat
     5281 ctgaatgcca tgagcagaac tctgttcgag gccatgggtc cggatggcac gctaaaggcc
     5341 tttgtgcact atccggcgaa tacttttccg ggacaaatcg aggagctcaa ggacgacact
     5401 cactttaatt cctatggagc acttgaactt gcaaagtgtg ttgtggaggc gattcgggaa
     5461 cagaatctgc cgctggcgaa ttttctgaat cctgatccgc agcgtttcga tccggcgcat
     5521 ccagatcctc cctcgatctg ggagctcccc acggatccct tttggtcaca gcaaaagcca
     5581 tacgagcgct aggtatttgc gtctcgtcct gcacctcgga ttgaaaactg acttcaccca
     5641 ggaggctgga ttttgaagaa ttctttcggg aaaaatcgcg ggctatgcgt ctcgctgctt
     5701 gcactcctgc tcccggtgat ggcccatgct cagaagcccg catccactgt cgcgccggga
     5761 gcggtcgggt cgtttccttc ctggcagccg ggccaggagc cgccgacgtc gtgggtggat
     5821 gcctccaccg gacatcgagt ggtacgactc acgcgcgagc cggactccgc gagcttctat
     5881 ttcaacgtga acgcttacac gccggacggt aaggaaatgg tgtacaccac cccggacggc
     5941 atttcggtgc tcgatctcac gacatggaag acgcggtctg tggtcgaggg gcatgtgcgc
     6001 accatcgtcg tcggacacaa gacgcccagc gtgttctaca tcaagccgga agagcgaagc
     6061 ctttatgtca ccaatgtcga cacgggtgag acgcgaaagc tggctattat gccggctcgc
     6121 ggctcagtag cgaccgtcaa tgccgatgaa acgctggcgg cgggcaccta catcgaaggc
     6181 acaggcggac cggattacaa ccagcctccg ccgggtgacg ggcacgccca ggatcagcgc
     6241 ggaggatcgc agcagacgca caacatgctg cagccgctga acaagggaca aatgatggaa
     6301 cagcgtcttg ccgcgcggct gccgctggtt ctgtttgtga ttgatctgcg taccggcagc
     6361 ttgcggcccc tgctgcacag cactgactgg atcaaccatc tgctcttttc tccgacggat
     6421 cccaatcttc tgatgtattg ccacgaaggg ccgtggcaga aagtcgaccg catctggacg
     6481 attcataccg atggtaccca caacacactc atccacaagc gaacgatgtt tatggaaatt
     6541 ttcggacacg agttttgggc tcccgacggc aagaccgtct ggtacgactg gcagacaccc
     6601 aagggtgaag atttctggct ggccggctac aacgtggaga cgggaaagcg gacagcctac
     6661 cacatgcagc gcaatgagtg gtcgatccac ttcaacgtca cgcgcgacgg ctcactcttc
     6721 tgcggagacg gcggcgatcc cggccaggtg gcgcatgcgc tggacggcga gtggatcgag
     6781 ctgttccatc cggagatgat caagggccag ggaatcaacg agcctggctt ctggcagccc
     6841 ggcgtttttc acagtgagca tctggtgaac atgtcgcggc ataactaccg gctggagccg
     6901 aacgtgagtt ttactccgga tcagaagtgg gtcctcttcc ggtccaacat gtttgggcca
     6961 acctatgtct tcgccgcgga agtggcaaaa gcgaaaaccg gcgactaacg gggttcttac
     7021 aggagccagc ggccgtcagc ggttttccaa tcgggccgca agccgccgtt tcgcctccgg
     7081 ccactccgac gcgacgatcg aatatcgggc ggagttgcgg gcgatgaaat cggaggccat
     7141 gcgatgagcg cgaaggatgc cttcaaatct ggctccgatg cgttcgatag cagcgcgaga
     7201 acgctcgttg cgctcgtcgg tgtgaaggca gacgcgcagc acgccccaac cttcaaaggc
     7261 gtgggtcagc atcagaagct ttgcttcggt atttgctggg gtgcggatcg aggagggctc
     7321 gagccaggtg taaccaatct cgcatacatc gggatgcagc cgaccatggc gctcgtggtt
     7381 ttcgggccag ggaaagcgtt cgatgtcgaa gaagcgcgtc gagccgataa ccgctccatc
     7441 ggcctggcgg accgttgcaa acggaaccgc aacgccttcc tgtcgccagc cgatcgctgt
     7501 gtctacatat ttcgtcatct tcgcgactcc ctggggaacg acggtccagc gatagagatc
     7561 gccggcattc gatgcggcgg cagccagggg ctcgacatgc cgggcttcaa gcggttccag
     7621 ccgcacgtgc ctgccgctga gagtgaaaag ttcctgattc atcgtctggt tctccctgtg
     7681 tgttcagtgc gtcctgacgg atctccatag aggattccgc ggccatgggt cccgacgtac
     7741 acgcgcccat agacgcgggg atcgcccgtg atctgcagga tgagacccca ctgatgctga
     7801 tggtcattga tacgggtcca ggttctggcc tcgtcgatgg aacgaaagat gccgtactgg
     7861 ccctccgtgg ttccgacaag atagatcgtg gggtaggtgt gacccggggc cgctttgccg
     7921 aagccaaaag cctctatctc tgttgcactg ggtatgcgct ggaacgagat ctcctcgcct
     7981 gtctgatccg tgaggttcgc atgatagagg ccgtcaaaag ccgcgaacca gaggtcgccg
     8041 ctgcgtcccg gcgcagcgta gagatggtcc tggcccccgc gtggatcgcc gcgcggagca
     8101 ggcgatgcag ggggagcact ttgcagcgtg aagtgctggg ccgcaaatct ggctgcggcg
     8161 tccgtgctgc ggtacaaagt gtggtccaga agcgaagcgg cgtagaagat gtcggaatcc
     8221 ttcggatcgg cgatcacccg tgtgccggtg ggcaaaccct gggcagtttt ccatgtcgct
     8281 ccacggtcgt gcgtgagcga cggcgtttcg ccttgggggg tccagaccca cgtcgcaccg
     8341 tccgcggaga cagcgatcga tcctgcatgg ctggctggcg acggagcctt tgaggtgggc
     8401 ttccaggtat ggccggagtc ggcagaatag ctgatgtttt cccccggctt gtgctcggcg
     8461 ctgatgccga cgcgcaccag aacttcgggt ctaagcgggg ccgacacgat cccggttgta
     8521 ttgccgaaac ggggcggcgc cgaggagcct tctggcgcgg gacgatccag gtcccaatgc
     8581 acaaatccgc cataatcgcc aattgcgctg atcagagaag cgccatttac cgggctgtcg
     8641 agctgcagtg ccacggtttc ctcgatccct gggaccagaa tgctccagtg tgtggactgg
     8701 ccgcgatcga cggcgctcag gtcaaacgtc tccagtccgc catacccggt gctgaaaaca
     8761 gcgtggtccg agttggcggg gtcgatctca acatcgaaca tccagtggat tggggtcgtg
     8821 tgcacatagg gcgcaagcga gtcgtcgacg atgccgctgt gcacgctgcc gaagagcggt
     8881 ttccacgttg cacccgcgtc ggtggaacgg aagatcgtct cgccaccatc gaatcccgga
     8941 cggccaaacg tgctggcgat gacgttttga ggatggtgcg cgtcgacagc aacggcggcg
     9001 tagccgaatt ccttgctgcc tcgcacggga tgttccggcg tgacatcagt ccacgcgccg
     9061 gtgtgcgtgt tcagcttcca tacggcaccg tcggtcatgc gcgaagggcc gggcgcggtg
     9121 ccataagaga tatagagaaa tccatcggac gaaagggaag agcgtgttgg ccgatattgc
     9181 gccggagcat tcgcaaccgg ccgccatgtt gctccggcgt ctttcgaaac aaagagggtg
     9241 ggccgattca tgagagaaac gccagcgtag atcgtctccg tggccttatg cgcatgtccg
     9301 ggtttcgaaa caaagtgaat gaacacgatg ccatcgccgt gcgctggggc ggcttccgtg
     9361 atgtcgggaa aactcgcgac gcgcgaccaa ctggcaccgc ggtcgtgtga gcgccacaag
     9421 ccatcgtgcc gcgtgcccag aaagagaata cgaccatccc ggggatcgac ggcgagcctt
     9481 tctccgttgc cccgaccgtc ctcattggcg ccgaacttga tcgggacatc cgctcgctga
     9541 aacgtccggc cgcgatccgc ggagcgaaga atcgctccgt ccggcgcgct cgagttggta
     9601 taggtgccac aggccatgta cagccggtcg ggatcagacg gatcaatcgc gatcgactct
     9661 acgcccatca gattgtggtc gcgctctccg atccagtcca gcaagggttg ccagcgatgg
     9721 ctcgcctgat tccagcggta cgcgcctccg atgtccgtgc gtgcgtagcg gacgccgggt
     9781 gccgaagggt gaaagataat gccgtcgacg aacccgccgc cgacgatctg cacgttcttc
     9841 caggtgtagt gttccgaggg cgtgcgctgc gccggacctg tgacggccgt gaacagaaac
     9901 aggcaggctg cgaaacggga gacgttcaaa cagcagtgat ccttaactgg aatcgcggag
     9961 acagtgtggc accagtttat tgcacatcga ctctagcaat gcacgcccaa gagaacctta
    10021 gatcatttcc ggtgaaggag cacagttctc gggaaacagg cagcgccacg acgaatcaag
    10081 gcagagccag cgctgtgtac tgaaccagct ggtcggcagc tcggggatgt gaagcaggcc
    10141 gtgccctcgg atgcagaaca actcggtccc gttccacgac aggaaattgc acgatgcctg
    10201 gccgacgcgg gccgggctgg atagctggcg caggcattcg gggcaacggc ggcgctgatc
    10261 ctgaagggcc cagcggaagc cgaagagaag cgctggaaag gcggcgccca cctgcaggaa
    10321 aaccgaagtg gagccggccc aggcggggcc tcggccgagc gcgatcgtgg cgagatagac
    10381 cgcggcgacc agaagcagca gcttcaggac cagaaagctc caccgccgca ctgtgatgcg
    10441 gaagaccatt ctgccgcgat accggggata gtctcccaac ggaagagcag tggtcgccgg
    10501 cagcgccagg caggcgagca gcaaagcgaa aaggaaattc gaagttggct gccaggtgag
    10561 ccacgagatc gaaacgcagt cgaagacatg ggtaatgccc cagcgagttt ccagcattat
    10621 gcgcacaccg ctgcgcgccg ggggaaacgc cgcgtcgcga atgcgtgcga atgcaaatcc
    10681 gcgcgcgtgc ggcgacaact gtcgcagagc ggcgtggtcc tggaacagcc aaccctgcat
    10741 cgcgccgggt agccgagggc tatcgtcgtg caagacggca accagcaccg attttccgtc
    10801 gattgtggcg gactgtccga cgatgcgagg gtcgctgcga tacaaggtgc gccatgcgga
    10861 acgcgtcagc acaagagtcg gtccagcgcc gccggacgcc tggtctggaa cctgtacacg
    10921 cagcagccgg aagaaattcg cgctggctag tccgatcgag agccgggccg tcgattcatg
    10981 agccagcaca acgtttttaa ccgtgggatg gtaccaggcc aattcagaaa atagcttctg
    11041 cgtattggtc tgccattcct ggtattgctg cagcgagatg ccgggggact ccgcgcctgc
    11101 gtagccggcc ggcgagatcg tgaccaaatc ggcggcttgt gaggggaaga gcggcaacag
    11161 cgaacgccgc gcgcccggca aaatcatgca aagaatcaac gccgcaccca tcaaagcggt
    11221 taacgttgct acgcatcgac ccgccgatcc ggcctgccag gcagaagctc cgtgtgagcg
    11281 cagatgatcg cggcacagcc accatgcatc aggaaaagcg ccacggctga actcgagagg
    11341 agttgtgccg ggagaatcgc tcaactctgc agcggaggca cgtcgcaggt ggtaaatctc
    11401 ggcatcccat tcagctctcc aggcggcgcg attcgcccgc ggcaccagaa gcgacgccac
    11461 gcgcacgttg gcgcaggcaa aaatcgtaac cagttcgtcg cgcttcatgc gcgctccatc
    11521 acaggtgtgg ggatcatctt ttccaggagc ggatagcgca cccatgcggc ttccagcgta
    11581 gtcctgccgg tacgcgtcag ccgatagtat ttgcgcggcg gacgctgctc ggtgtcggcg
    11641 atcgactgtt gctcccactt cgaaacaatc agtccgtcgc gctccagccg ccgcatggcc
    11701 ggataaacgg taccgctggg cagacccgtc atctgcatca cgctgaagcc gtagatgtgg
    11761 ccggcgtgga tggcctgaag aatcatggca gcggtgtgcg aaagcttcgc gtcagccggc
    11821 atagcgggga ttatacccta tgtagcattc tgttgactcg ggtaaaagaa gtatgctaca
    11881 tagggtgctc agaaccgtta tgctcgagct ccagcatctg tcccgaagct accgcggcat
    11941 ccccgccatc gaggacgtca gcttccgggt cctggccggc gagattgtcg gcttcctcgg
    12001 cccgaacggg gcagggaagt caacaaccgt gaagatcatc acgggcctga ttcgccccaa
    12061 cgacgggcgc gtgctcttcg agggcaaaga catccatacc gacctgatca attggcgcgc
    12121 gtcctttggc tacattccgg aggaagcgca cctctacgct tggatgtccg ctatggaata
    12181 cctgcagctc gttggccgac tgcgcggcat gcgcgagcgc gtgattgaag aaaaagcgac
    12241 agcgatgctg gcgctgctcg gacttgaatc ctggcgccat tccccgatga ctgtttactc
    12301 caaaggcatg cgtcagcgtg tgctgatggc cgcagcgctc atgcacgacc ccaggatgct
    12361 ggtctttgac gagcctttgt ccgggctcga tgtgttgtcg gcgcgactct ttcgcgacct
    12421 gctgcacgag ttggctgccg caggcagggc ggtgctgtac atttcgcacg tgcttgaaat
    12481 tgtcgagcag atttgcgacc gcgttgttgt gatcgcacag ggcaaagtgg tggcggatgc
    12541 tgcgccatcg gaattgaccc gcttgatgga attgcccagc ctggaacgtg tattcgctca
    12601 actggtccgc cagcaggata ctcgctccgc cgctcgcgac attgtggaag tgatgcgaat
    12661 cggtcatggc tagcccagcc cgccaagcga agccgccccg ccatgaggtc ctctccctgg
    12721 aagtgctggc acgccctgaa tcgcagagtc cggagggatc gccggggcag ttccgcgtcc
    12781 tggtgcagca ttttctcgat cgatttttca ataacgaatt ggcgtcaggc gagggcgacg
    12841 ccaagacgcg actggtgcag gccgcctgca cgatcgggat tcctggtctc gtcgttgcta
    12901 tgtatcttta tcccgtctat cacctgccgc gggggcacgt tggccggtac tgggggccgc
    12961 gtccgttttg gtcgcaggtg ggcgaccact atttctacgt ggtctattcg atggtggcga
    13021 tggggctggt gaccatcttc gagtgggacc tcttgttccc agacctcctc gacgtagtcg
    13081 tgctttcccc attgcccaca cgctcgtgga aaatgctagg cgcacgggtg gcggcgatct
    13141 tcatcctgct gggggcagcg ctgttcgacg caaatttcct tgcgcccctc gtcctgccag
    13201 cggccgttga tccgccgcat ctggtcagat tctgggcggc acatctgctc gccgtggcag
    13261 ccagcggagg tttcggagca gcattctttc tggcgctgga gggagcgctg cttggtctgc
    13321 tcggagatcg cctctttcgc aagatctcgc tttggctgca gggcttgttc gtttgggggc
    13381 tgctgacgct tctgtttctt taccccgcga tggcggggac catgagcggc ctgctggcac
    13441 cggactcccc cgtcgccgcc tgggtgccat cgttttggtt ccttgggata taccagcgca
    13501 ttctcgatgg gccgtcgacg ccggcaatct ttgtacggct agcccacatt gctgaggtcg
    13561 cactgatcgc ggcgatcgcg gtggccattg cctcgtatcc attggcgtgg tggcgcaaga
    13621 cccgcagcct ggttgaggga accgcaaagc gggagacacg gaactgggct gttgcgccgg
    13681 tgcaatggct ggtacacctt gcagtggcgc gcacacccgc aagccgcgct atctggcagt
    13741 tcatcggtca gaatctcctg cgtattcctc gttaccggat ggtgctggtg atgtacggcg
    13801 gtatggggct ggccctgctt tttgcgactg tgactcgcgt cgcgctgcaa cgcggacatc
    13861 tcttgttttt cttttccagg gatggacttc gggccaccgt gccgatcgtg gcgttctgga
    13921 cggtgtccgg tctccacagc actttcctgg cccctgcaga tcagcgaggc cgctggatct
    13981 tccgcgcgat cctcgggaaa gctgggattg cggagctagt cgcggcgaag agatgggttc
    14041 tcttctggag cgcgctcctg agcatgacag ccgccggatg tgcgtacgcg ctcgccgcag
    14101 gcccgcgcag ctggttgttc gcagcagatc aactcctggt ttctcttggc tggagcctca
    14161 ttctgacgga ccttttcttc ctcaacgcga cgagcattcc gtttactggt gctcgcccca
    14221 actctgcgac gaattttgca ctgctgctta ttccttacct cgggttcttc ccggcagttg
    14281 tcatgttcac ggtggcgctg gaacccgtgc tcgaagtcag cgtcggacat ctggcatgcg
    14341 gcgtcggcct cgtggccgtc gcgcacctga tcctgcacgg gatgcatcgc agccgtatcg
    14401 ctgaccatgt gcacggcatc gagcccgacg aagacgaaga ggaatttcct cttcgtctcg
    14461 gcctgcgcta ttagtgccac tcgtcggcac tggggccata gattgccggc actttcgctt
    14521 cgttcaggcg taaatacacc gtgaactggc cgcgatggtg agccagatga ctcagcgcgc
    14581 cgtcgcgaat ttgcgaatag cgcggacctt cggcgaggac ctgatcaccc atcttcagtc
    14641 gccacggctt catcaggtgc tcttctgttg tgttttcgag agcgttacga gaacgcctca
    14701 ggccctcgtc gagcatggcg aggagttctg cgcgggttcc cacggcatgc gtgcgaaacg
    14761 aggcgccacc agcgttcaga tcggtctcgt cgttatcgat catcatcgcc acccagcccg
    14821 gcatgcccgc aaccagcgac gcaaggtagc cgaactccat cgagcgctca tggggcttcc
    14881 atgagttctg tccctcaggc atgcgctcca gtgccttgcg tgacgaggct gcttccgact
    14941 ccaactgctc caggaaaaac ttctttaggt ccatggcgaa tcctccgagg aggccattct
    15001 cggcctcagg aggaactcta atcgttaaaa tatgacatcc aatgtcataa ttagcgaaat
    15061 atccctggtg gactatgaaa gccgatcgtc tgctctccgc gttgtttctg ctccaggctc
    15121 acgggcgact cccggagcgc gagatcgccg aacgtctcga agtgtcgcag cgcacagccc
    15181 accgagatat ggaggcgctt tgcgctgccg gcgtgcctct gatcgcacac cggggcgtcc
    15241 gcggcgggtg ggaactcgaa aagggttggc gaacccgagt gcccgggctg gacgagacgg
    15301 agctgcgggc cctcctgatg gcccagccca gtggacttgg agaccggcgt ctgacagcag
    15361 cggccgagcg tgccttcaat aagttgctgg catccttatc tccgcccatg cgcgcccagg
    15421 ccgaatcgat gcgggttcgc ctccatgtcg atgccacagg ctggcgtccg acgaccgagg
    15481 acctctcagc cctttctgtg gtccaggatg cgctggagcg ggacgtaaag ctaacgtttc
    15541 aatacgcagg ggtcaaaggc acgacaacat cgcggacggt tgatcccctg ggactggtgt
    15601 cgaagcagac ggcttggtat atggtggcgc gtgcgccgga agggatgcgg acgttcaggg
    15661 tttcccgcat gcgcgatgct gtcgctttgg ctatccgttt tgaacggccg cctcggttca
    15721 acctcgcggt gtactggaag cgcagcacgg aagccctgca aaccggaaga cagaaatata
    15781 aggcgatcct cgggctgagt tccgaaggag tcacggcgct ggcgcaatgg gtggcgatga
    15841 aaccgctcga aggcaaaaaa cttgattcgc tccaaggatg gcaggccttc gtcgtggagt
    15901 tcgacagctt ttactcggcg cgctttgttg cgttgggcct gggaggatcg gcagtcgtgc
    15961 tgggaccgga ggatttgcgc cgcgacgtgg gagaacaagc ttcggctgtt gcgcgcgcca
    16021 ctcaggaatt gtcgtgaact gagctatgcc gtgcgggcgg ccgccgcatt ctgtaacccg
    16081 cgcgcctcaa tctcgaattc gcgcattttg tacttctgga tcttgcccga gagtgtagcc
    16141 gggaattctg ttacgaaacg gatgtgttcg gggatcttga aataggcgat ctgatcccgg
    16201 cagaagattc gaatctcttc ttcggtagcg gtctggttag gcgccaggcg gacccacgcg
    16261 accacgattt cgccgagcct ttcgttggga atgccaacca cctgaacctc actgatcttc
    16321 ggatgggtgt acaggaactc ctcaatctcg cgcgggtaga tattctcacc gcctcggatg
    16381 atgatgtcgc gcgatcgccc agtgagatga atacagccgt ccggatgcat cacgccaaga
    16441 tcgcccgtgt agagccatcc gtctgcatcg atggagagcc tcgtggcgtc cggctccgcg
    16501 tcataccctt tcatcaccgc atacccacgc gcacacacct cgccctgttg tccgaccggc
    16561 agagttgttt tgtccgacgt ggagacgatc ctgatctccg tctgcggcat ggcgcgtccg
    16621 accgtattta cgcgaatttc gattaggtca tccgcgtcgg acatggtcac caccggcgag
    16681 ctctctgtct gaccgtaggc gatcaccaac tccgggatgt gcatgtcgtg cagcacccgc
    16741 ttcatcagct cgatcggaca gggcgcgccg gacatcatac ccgtccgcag gctggtgaga
    16801 tcgaactcgg caaactcggg aagggccagc tctgcgacat acatggcggg caccccgtag
    16861 agcgaggtgc agcgctcgct gtgcaccgcc tggagagtgg cacgtgcatc gaaggtccag
    16921 ttgggaaaca ccagcgctgc gccggtattg agggcactca tggtgccgat cacgcatccg
    16981 tagcagtgga agaggggaac ggggacgcag actcggtcat gttcggtgta gtgaaatccc
    17041 tgcgccagga actggccgtt attgacgatg ttgtgatgcg agagcatcac acccttgggc
    17101 aggcctgtgg tgccggaggt gtactggatg ttggcgatgt cctgcgcgtc gacgcggtcc
    17161 gggatctgtc ctggcgcctc cagtagagcc gtccagtctg gcgaatcgaa gaagactgtt
    17221 tgccggagtt ccagtgtcag gttgtggcgc gcccgcccca ggatttccgc atagtcggca
    17281 cgcttgtcat gactccacaa aaagagggcc ttcatgcgtg accgcgtgag ggtgtactgc
    17341 aattcgtgcg agcggtaggc agggttcacg ttgaccaggg atgccccggc acgggctgtg
    17401 cccatgtgca tcatgaccca ctcgacgcag ttggtcgacc aaa
//
LOCUS       DASZYN010000646         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_735709, whole genome shotgun sequence.
ACCESSION   DASZYN010000646 DASZYN010000000
VERSION     DASZYN010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735709"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VHE33_16605"
     CDS             complement(<1..959)
                     /locus_tag="VHE33_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99128.1"
                     /translation="MRQCGRWLVVTLLGFAASWCGAQQQAEPFPGEPANQQQSRPQQQ
                     AEPFPGEPASQPKNQPAQPSTRAPQDFPADPNSDLPTFRVSTTVVVVPTLVEKPNGDV
                     LYGLKPQDFSVYDNGVEQKVHVDDDLDTQPISLVVCIERGRDAPLEFDQIGKLGPLLE
                     LFTRNGRGDMALVAFDSKATWVEPFGTDQDTISKDLKHMPAGDGGAAILDAAGYSVNL
                     LEQQPADHRRILLLISESRDHGSHHFDIPQLVERIGTSNTLVLSLVFSPGKAEIIDWA
                     KGNTGGGTEMNILAPLMMTVNAMRRNTPKTLAELSGGEYDPFT"
     gene            complement(998..1942)
                     /locus_tag="VHE33_16610"
     CDS             complement(998..1942)
                     /locus_tag="VHE33_16610"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HVT99129.1"
                     /translation="MSTPFEWTNPRTWPLIFDAWLAITVAGGLHSLWKWNRVKRGTGW
                     PVVPGRIVWTEVRAKDDSWSFGQRNGENFTAEIGFEYSLEGQSYSGDFSRDSGTEDEA
                     REFIRDLDGKMVMVSYNPRKPTDATLTDTALADVLKTRAFDPVEAWAAATRRELPEWV
                     KPWLWLLIAISAAGFAVSLWVHIEALADREIAPQSLFWILHFVLFVVCFPAGLVAIRR
                     VGSTQRRDFWKAVLRGSPLWVRLVLYGVMGYETIIVLLAFVHNPLPGNQDGTSATDWR
                     TFSGLWLVFYFAAFAIFYSAAIAEKTLLEPPRQPPVQP"
BASE COUNT          436 a          697 c          571 g          399 t
ORIGIN      
        1 ttcgtgaacg gatcgtactc acccccgctc agctccgcca gcgtcttcgg cgtgttgcgg
       61 cgcatcgcgt tcactgtcat catcagcggc gccaggatat tcatctccgt cccgcccccg
      121 gtgttgccct tcgcccagtc gatgatctcc gccttccccg gcgaaaacac caggctcagc
      181 accagcgtgt tgctggttcc aatccgctcc acgagctgcg gaatatcgaa gtgatggctg
      241 ccgtggtcgc gcgactcgct gatcagcagc agaatccgac gatgatccgc cggttgctgc
      301 tccagcagat tcaccgagta ccccgccgca tccaggatgg ccgcgccgcc gtcgcccgcc
      361 ggcatatgct tcaggtcctt gctgatcgtg tcctgatccg tgccaaacgg ctccacccac
      421 gttgccttcg aatcgaaggc gaccagcgcc atatcgccgc gtccgttccg cgtgaacagt
      481 tccagcagcg gccccagctt gccgatctgg tcaaactcca gcggcgcatc gcgcccccgc
      541 tcgatgcaca ccaccagcga aatcggctgc gtgtccagat cgtcgtcgac atgaaccttc
      601 tgctcgacac cgttgtcgta aacagaaaaa tcctgcggct tcaacccgta cagcacatct
      661 ccgttcggct tctccaccag cgtcggcacc acgaccaccg tcgtgctcac gcgaaacgtc
      721 ggcaggtcgg aatttggatc ggccggaaaa tcctgggggg cgcgcgtgga cggctgcgcc
      781 ggctgattct tcggctggct cgcaggctct ccgggaaacg gctctgcctg ttgctgtggc
      841 cggctctgtt gctggttcgc cggctcaccc ggaaaaggct ccgcctgttg ctgtgccccg
      901 caccaggacg ccgcaaagcc cagcaacgtc accaccagcc atcgcccgca ctgcctcatt
      961 gccttcatta catcagacac agacgaacga ggcgagatta cggttgaaca ggcggctgac
     1021 gtggaggctc aaggagcgtt ttctcggcga tcgctgcaga gtaaaagatc gcaaaagccg
     1081 caaagtagaa gacaagccac aaacccgaga acgttcgcca gtccgtggcg ctggttccat
     1141 cctgatttcc gggcaacgga ttgtggacga acgccagaag aacaatgatc gtttcatatc
     1201 ccatcacgcc ataaaggacc aggcgtaccc agagcggaga tccgcgcagg acagctttcc
     1261 aaaaatctct tcgttgcgtg cttccaactc gcctgattgc taccaggccg gcaggaaaac
     1321 agacaacaaa gagtacgaag tgcagaatcc aaaacagcga ttgaggggca atctccctgt
     1381 cggcaagcgc ttcgatgtgg acccagaggc taacggcaaa tccggcggcc gagatcgcga
     1441 taagaagcca aagccagggt ttcacccact cgggtaattc gcggcgtgtt gcagccgccc
     1501 aggcctctac tggatcgaac gcgcgtgtct tcaatacatc cgcgagcgcc gtatcggtca
     1561 aagtggcatc ggttggtttt cgcggattgt acgaaaccat caccatcttg ccatcgagat
     1621 cacgaataaa ctcgcgagcc tcgtcttccg taccgctgtc gcgagaaaaa tcacctgagt
     1681 acgactggcc ctccagcgag tattcaaagc cgatttccgc ggtgaagttt tctccgttcc
     1741 gctgtccgaa actccaggag tcgtccttcg cgcgcacttc agtccaaaca atccgcccag
     1801 ggacaaccgg ccagcccgtt ccccgcttga cacggttcca tttccagagt gagtggagtc
     1861 caccagcaac cgtgattgcc agccacgcgt cgaagatcaa cggccacgtg cgaggattcg
     1921 tccactcaaa cggtgtggac attggcatca ttcaggtttg ctccgcccag gcctgaacgg
     1981 cgcataagga tcagcccccg gcagcctccg cgccggtcga gcgtcgatcg cgtcgcgctc
     2041 ggcaaagaac gccggcgtcg gattgtagtc gtaatcgaac ggcagcggcc gctccggctt
     2101 ccc
//
LOCUS       DASZYN010000647        19000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_735858, whole genome shotgun sequence.
ACCESSION   DASZYN010000647 DASZYN010000000
VERSION     DASZYN010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 19000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19000
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735858"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1104
                     /gene="pgeF"
                     /locus_tag="VHE33_16615"
     CDS             82..1104
                     /gene="pgeF"
                     /locus_tag="VHE33_16615"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HVT99130.1"
                     /translation="MPSPRTVSADPIDELVGQGELVSNPRSALSRGRRREPQPEHKGI
                     EPQAQRRVAKSRVEVVRAAGWNAWPWLVHGFSTRTGGVTTVYRPDQHAGELNLGFTQT
                     DTRESVVENRRRFLKALGAPASMKMVTLRQIHSSSVLTVGEHELSESCQGDGLMTNQP
                     GVLLAIQTADCIPVLVADVKKKAVAAFHAGWRGTVKRIVESGIGKMRVAFRSRPEDLV
                     AAIGPGIAACCYAVGEEVRSEFGSQFSYAAELFHEVSDTDPIREKYPMLFLTARAPGH
                     SELGPSLHLDLMEANRRQLLDAGLRAEAITVIGDCTRCQNNRYFSYRAEQGFTGRMLS
                     VVGIRR"
     gene            1303..1776
                     /locus_tag="VHE33_16620"
     CDS             1303..1776
                     /locus_tag="VHE33_16620"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HVT99131.1"
                     /translation="MSKSRIENQKHATDKVSKISSEIAAQTRGDAFGKFAAAASAWLG
                     SKWAFLGAISIIAAWAITGPIFHFSDTWQLIINTGTTIVTFLMVFLIQNTQNRDARAI
                     NLKLNELIHTSAAAGDQMMDIEKLSDEELDALQARYEKIRAEWMERNKGQGGDKS"
     gene            complement(1920..2837)
                     /locus_tag="VHE33_16625"
     CDS             complement(1920..2837)
                     /locus_tag="VHE33_16625"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVT99132.1"
                     /translation="MKTLDEAVSSKRSFVVPEKILVATDLEDSEYLLPHAIAQARACG
                     STLTLLHVIPPGQAVPLDAAAIPYLDVAEMKAEAEQTLKRMAEKAQAAGIECDILVTE
                     GNPREQITTLARELHAGRIIAGTHGRRHLKKLLLGSVAHEILRSAEVPVCTIGPHARE
                     ASSFGAPQKILHPVSLCAGYESSARMAIEMAEFYRADITLLHVLSRSVNSQPDADRVV
                     EWTRSELKRVIPDEAPLWTHPSVQVEIGDVVDEVLNVAAEMSADLIMLGVDANVSFWP
                     IRGDDTVYSIIARAKVPVLSIRHQPGMVM"
     gene            3079..4203
                     /locus_tag="VHE33_16630"
     CDS             3079..4203
                     /locus_tag="VHE33_16630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HVT99133.1"
                     /translation="MATEESVLETCKKRGISRRRFMEFCGTMAATLALPKGYGQTIAG
                     ALTAKRKPTLVWLEFQDCAGNSEAMLRSTHPTVEEVVLETLSWEYHETIMAGYGKHAE
                     AALRRVVEEEKGKYLVVVEGSIPAGDDGVYCTIGGRTALDIAREVCTNAAASIAIGSC
                     AWDGGLPKANPNPTGAMGLHEAVPGITVVNLPGCPHNPTNTAAVLVHYLTFGVLPAMD
                     GYNRPLFAYGQLIHDQCERRAHYDAGRYVQAWGDEGHRKGWCLFKMGCKGPEATYNCP
                     TVRFNDSTSWPVKSGHGCIGCASSRFWDTKSPFYERLPHPAGFGVDVTAGQIGWTVVG
                     AVAAATAAHAVGHVVRDHFHPSGEGAAKEETAEKTTTGVN"
     gene            4205..5986
                     /locus_tag="VHE33_16635"
     CDS             4205..5986
                     /locus_tag="VHE33_16635"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HVT99134.1"
                     /translation="MARVVIDPVTRIEGHLRIETEVNGGKVTDAWSSGTMFRGIEIIL
                     RGRDPREAWIWAQRICGVCTTVHALASVRAVENALGIEVPDNARLVRNIIAGAQNVHD
                     HVVHFYHLHALDWVDVTLALKADPAKTSQLAQSISEWPKSSTTYFKGVQDRVKALVAS
                     RQLSLFSSGDWGHPAYQLPPEANLLAVAHYIEALDMQRDFIRIHAVLGGKNPHLQTYL
                     VGGMSTAIDGNEPEAVINPERITLFNELAARAKTFVQQVYVPDVLAVASFYKQWFGYG
                     EGLGNFMAYGDLPSGSVNDPAGFFFPRGVILGRDLSTVHPVDPGKVSEYVTHSWYDYS
                     QGDQVGRHPSQGETNPRYSGPKPPYLTLDTNAKYSWLKSPRYDDKPMEVGPLARTLIA
                     YASGDPQVKKTVDAVLAKFNAPPTALYSTLGRIAARAIEADLVVQKLPEWIAQLDNNM
                     AHGDVRIHNGDKWDPDTWPKSCAGFGLEEAPRGSLGHWVEIENKKIVNYQAIVPSTWN
                     AGPRDAHGQRGAYEAALLNTPIADPERPLEVLRTVHSFDPCLACAVHVVDGKGRSYGR
                     EVVAVAEPEGSMSLSPVPAALCSIGGE"
     gene            5986..6774
                     /locus_tag="VHE33_16640"
     CDS             5986..6774
                     /locus_tag="VHE33_16640"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HVT99135.1"
                     /translation="MATLVRPAPKKHVAAGEEHLVPVYVWELPVRLSHWGIVAAVTVL
                     TWTGLYMNHPFLIFHGPREWTMGTVRFIHELAGFELIGALLVRIYWFFAGNHWARWRQ
                     FLPLTQRQWKSLKAMFRYYTFQQKKPFEQIGHNTLAGLTYIIVYFLIACECVTGLVLL
                     ATVGGSHTLTFFVGWIPHIIDIQWLRFVHFLFMFFLMGFFIHHVYSATLVAMTERNAE
                     MESIFSGYKFVDDQTIDEELEIARLEPQALFSKRKIHRTRDWDR"
     gene            6771..7322
                     /locus_tag="VHE33_16645"
     CDS             6771..7322
                     /locus_tag="VHE33_16645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HVT99136.1"
                     /translation="MSTPRIAVLGLGNLMRTDDAVGMLALQRLRSDPRFPQSIPLIEG
                     GTLGLDLIYPLDGFTHVLALDAIDAGVQPGTVLRYSGDAIADLPISKSVHLLGFSDLI
                     GSMRLIGNAPEEIVVLGVQPKTIDWGTELTSPVEAALDILIEQVLDQIGQWSEENEGM
                     PAVSNRVEATVPQLAAAEEAWYW"
     gene            7421..7780
                     /locus_tag="VHE33_16650"
     CDS             7421..7780
                     /locus_tag="VHE33_16650"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HVT99137.1"
                     /translation="MHEVGIATSILSAGHRETERRPGARLVGIGVRIGVLSGVDVDAL
                     RFAFECITAETEDEKVVLTTEQCPRVNRCEDCRHEFSSRQSSPFSDAPCPRCHSARTS
                     FVSGDQLDIAFIEVEEP"
     gene            7777..8454
                     /gene="hypB"
                     /locus_tag="VHE33_16655"
     CDS             7777..8454
                     /gene="hypB"
                     /locus_tag="VHE33_16655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HVT99138.1"
                     /translation="MTVVRVEQKVLNENQRIALELRRRFAEHDILCLNLISSPGSGKT
                     TLLEQTLAGLGPEEKIAVLTGDLQTENDAKRLARYGFPVQQIVTGGVCHLDGKMIERH
                     LQAWSLEDIDVLFIENVGNLVCPSSYDLGEEAKIVLLSVTEGEDKPLKYPSIFLKSSL
                     LLLTKVDLLPYVPFDIEAAKANARQINPAIEILEVSCSTGKGMDEWRTWLRNRRARSA
                     ATPANGR"
     gene            8796..9716
                     /locus_tag="VHE33_16660"
     CDS             8796..9716
                     /locus_tag="VHE33_16660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HVT99139.1"
                     /translation="MRRFLFSIACLLLLTGFAGTRLALAAGGGQQPAATSQSKAAVDS
                     TNFVGQETCATCHEDVVKKFGTNPHSRLALEHAGKGVTCESCHGPGQAHVEGGGDVTK
                     IRRFDKLGTKQIDATCLGCHAGAHPNFDRSPHAKADVSCLGCHNVHDSKTPEHLLKAE
                     QPKLCFQCHTDQKANFDMPFHHPVNEGTVSCSDCHNPHGTFNKANLKAIADQNAVCTK
                     CHTDTRGPFVFEHAAVKAEGCTGCHTPHGSQNARMLNMPSINMLCNQCHSPVAEATVH
                     GMGAGSTASQPCISCHTMIHGSNVSQAFIR"
     gene            9736..12294
                     /locus_tag="VHE33_16665"
     CDS             9736..12294
                     /locus_tag="VHE33_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99140.1"
                     /translation="MKTTGWFSWLSNPRRSRTLAGRVGQGVGVAIIGMTTGVALAQAP
                     APDTQPVAPNGYTLHQSVDLGGRMTNVTGSGAMYDTLVNMRSGPRVLGETFELRALPG
                     TKHMLVDSLTAFGSGFGGDPNNFARLSFSKGNLFDFSGLFRRDRQYFDYDLLGNPNIV
                     PGRSIPIGPTSAPTGSLAWPQVKVSPVMFNTVRRMTDTNLTILPLSTFSYRIAYSHNT
                     FEGPALSPSYAIAKYDALLRQYQRNGSDDYTAALDWKPLPQTRVTIEENVNRYKSDTF
                     FTLNPNQFMVQEADGTKVDLGNWDSETPYGASACNANSLGTTPVLSAPQTPGGLPVIN
                     AACAVVTSYLRTQPTRITIPTSIIRLQSSSIAKVTMNGDFRYTLANMEMPNYYENFKG
                     LNGAVRSSTYSGGSASAHRAVLSADYGIVWQATDAISISDQFDYSSVQQPGWSNIPAP
                     ATLSTPTTAGNQTINYSGPLTPGTGALPHGIEGFRYPGFFGQSYITNSATLSWDATPR
                     TRYFITYRYSDHTIGEGGDHTVRPVDTSVDPFQGTVEIKENAGILGVSLRPATNWDIN
                     GSAEIGYLDNAFTPVGSRQLQRYRVHTTYKPKRWMVLSGAFNDLERHNNTFNNEEEIA
                     AGAIYYGPINHIDHTRIASLGATLNPNEHYGFDMNYVWNDVYTATNTCFTSGAAANLP
                     GTATLKSDGTPDVCPGVFARGSTTILVDWHARAYEDIPVQYGSASIHITPNNKFRSDI
                     GYRIDSSNGSRFYQDPRDVAGSLVSTWQQPFVDVAYTLHPGFIWKAEYNFFGYGEGGP
                     SGPAYCSLSTGLTASVVPCASLPYPTGLTEASSGLTAPRNFHANNVTLGFHYEF"
     gene            12359..12679
                     /locus_tag="VHE33_16670"
     CDS             12359..12679
                     /locus_tag="VHE33_16670"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVT99141.1"
                     /translation="MKQNVRIAVLTLLSASLVLPAVAQQAGEATYKAKCAMCHGADGV
                     GNTTVGKNMKVRSLKSPEDIKATDAELFKQTKEGVGKMQGYAGKLTDAQIRDVVSFIR
                     TLQK"
     gene            12836..15001
                     /locus_tag="VHE33_16675"
     CDS             12836..15001
                     /locus_tag="VHE33_16675"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HVT99142.1"
                     /translation="MGTQAVNLPAECRSKGSRAHRHLLFDGLVLLFFITALSVLPQAR
                     AQKASDCLLCHGPSTGLKNSKGKSITISPSTHMKGPHSDLGCLDCHTGASEQTHNAKS
                     ASASCQTCHADSAKAMTTGAHTALGNPNDAQTCITCHGTTNTAKAVAGPQFCATCHAS
                     EVEQYRSSVHGRAQAKGNADVPTCQSCHGSAHAALPATDPQSPVSKQHLPDTCGSCHS
                     NPQLAAKYMFAEVRPVQAYRQSVHGRALEHGNTSVASCGDCHGTHDILPASDPKSKIW
                     KQNVASTCGNCHTGVFNTYAQSIHGQAVSKGVLQAATCTDCHSEHRILAPGDPGSPVY
                     MANVSQEACSRCHADTQLMAGFAMPQDRVPTYEDSYHGLAAHEGRQTVANCASCHGVH
                     NIYPSSDARSTVNHANLSKTCGQCHEDAGTRFAIGPVHTLSKTTPGGRLLDFVKIFYF
                     IVIPVTIGFMLLHNLIDLRRKARKILAGYRQHPGQIRLTLSERWQHVLLAVSFIILVI
                     TGFALKYPHAFWVKPIVGWEHHMPIRGWIHRIAGVVLIGASVYHMVYLAAKKSGRRWL
                     KDMIPDLRDAKEAIGTVQYNLGHRDQLPRYRRFNYAEKAEYWALVWGTIVMAITGVLL
                     WLNDWILAHLPHPATILAVSTAIHFYEAILATLAIVVWHFYAVIFDPEIYPIKWTLLT
                     GRAPEHEVREVEDEPASEKKLSDGSGPAQMPHPQSEGDD"
     gene            15141..15458
                     /locus_tag="VHE33_16680"
     CDS             15141..15458
                     /locus_tag="VHE33_16680"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVT99143.1"
                     /translation="MNRIRTAAMILLAASIAAPAFAQSAGADTYKAKCAMCHGPDGTA
                     STPAGKAMKTPSFKDPASVKSTDADLIAVTKNGKGKMPAYAGKLTDAQIKEVVSYIRT
                     LQK"
     gene            15572..16759
                     /locus_tag="VHE33_16685"
     CDS             15572..16759
                     /locus_tag="VHE33_16685"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HVT99144.1"
                     /translation="MRTLSSFGLAIFAPALLLIALTRPLYADVDCLSCHSDTTMQDAN
                     GKNVGVDANKFHAGVHGILKCGDCHKDIHDYPHPAQPAKVQCETCHADEASALAGSVH
                     SNKNEHPCTSCHGNAHEIVTKTDPNSAVYPLNIPRTCGSCHGNPAMAQKYHLPNVYAL
                     YMDSIHGFALGKEGLLVAANCTSCHGSHKILPHTDPQSRTNRANIAATCASCHFGINE
                     QYQQGVHGKAVAAGDKHAPVCTDCHTAHGIVEPTSADFRMQSTPICGSCHREKFSTYR
                     DTFHSQLGLLGGYVETARCWDCHQAHDVRPASDPNSPIAPAHLVQTCGRCHAGANASF
                     VQYQPHANARNRKLNPALYYIRLFMNLLLTSVLTFFTLHTVLWLIRSRFKGNGKLPGG
                     KNA"
     gene            16752..17654
                     /locus_tag="VHE33_16690"
     CDS             16752..17654
                     /locus_tag="VHE33_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99145.1"
                     /translation="MPEPTAAPAAADAVPAPAVEAVISPAAPEKERWFLRFTLAQRYL
                     HAVLFTSFLVLAATGLMMRFSGSPWAIRFAKLVDGFGTILFFHKFCALVLSLAFVIHV
                     REILARGVFKREKGIFWGPTSMVANWKDVKDLFGHMRWFLGLGPKPKFERYAYWEKFD
                     YWAVFWGMVVIGFSGYAMWFAPFFARFLPGWALNAALVIHSEEGLLAILFIFSIHFVN
                     THLRPDSFPMDMVIFTGRESEEEFKHRRPDEYQRMVAEGKAATRITDAPPKWLINFSR
                     VVGFTAILIGLTLLVLTLSAYFAE"
     gene            17667..>19000
                     /locus_tag="VHE33_16695"
     CDS             17667..>19000
                     /locus_tag="VHE33_16695"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HVT99146.1"
                     /translation="MSQWRRLKEDWLQPFFFYGNNLLSLIGGALTTAAGCVLVGFWIV
                     SIFGHAGSSNPYLGLILDLFLPGLFVFGLALIPVGIWLHRRRLIAAGQVPATYPTIDL
                     RNPAFRHGIEVVVIATFINFVIVGTATYRGVSYMDSPHFCGQSCHVMAPQWAAFQESP
                     HSSVDCVQCHVGSGMKSYIHAKVNGTKQLVEVTFHTWPTPIRANLNTLRPARETCEAC
                     HSPTRFVGEKLLVKTTFGDDEKNAMTRTVLVLHLGGVDSVSHFTGIHGHHLNNFEYVA
                     TDNTLQTILSVTATNSDGSKTEYISSDAKGPIQGVRRTMDCIDCHNQATHVFQTAEEA
                     IDRAMLDGTPDPNLPFIHKQGLQLIQATYSSQAEAGQKITSGLEDFYRTQYPQIWSSQ
                     RAQIEASARRLVGIYDRNVFPSMKITWGTYPNNIGHMAYPGCFRCHDGSHTS"
BASE COUNT         3757 a         6018 c         5609 g         3616 t
ORIGIN      
        1 cgccgggtgg aactggtcga gtggtagctc ggcgtccgat ctgggcctta ccggctcggg
       61 gcctcgatag aatgggatca gatgccgtcc ccacgcactg tttctgctga tcccatcgac
      121 gagctggttg gccaggggga actggtctcg aacccgcgct ccgcactgag ccgagggcgg
      181 cggcgcgagc cacaaccaga acacaaaggg atcgagcccc aggcgcagcg gcgcgtcgcg
      241 aagtcgcgag tcgaggtcgt gcgtgcggct ggatggaacg cctggccgtg gctggtccat
      301 ggcttcagca cccggaccgg cggggtgaca accgtctacc ggccggatca acatgccggt
      361 gaactgaacc ttgggttcac ccagaccgat acccgggaga gcgtggtcga gaaccggcgg
      421 cggttcctga aggcgctggg cgctccggcg tcgatgaaga tggtcacgct ccggcagatt
      481 cattcgtcca gcgtgctgac cgtcggcgag catgagctgt cagaatcctg ccagggcgat
      541 ggactgatga cgaatcagcc tggcgtcctg ctggcgatcc agacggcgga ctgcattccg
      601 gtgctggttg cggatgtgaa gaagaaggca gtggctgctt tccatgccgg atggcgcggg
      661 accgtgaagc gcatcgttga aagtggtata ggcaagatgc gggtggcgtt ccggtcgcgg
      721 ccggaggatc tggtcgcggc gatcggcccg ggcatcgctg cttgttgcta tgccgtgggc
      781 gaagaggtgc gcagcgagtt cgggtcgcag ttcagctatg cggcggaact gttccacgag
      841 gtgtcggaca ccgatcccat tcgggaaaag tacccgatgc tgttcctcac cgcgcgggcg
      901 cccgggcaca gcgagctggg gccgtcgctc catctggacc tgatggaggc caaccgtcga
      961 cagctcctcg atgcggggct gcgggccgag gcgattaccg tgatcggcga ttgcacccgc
     1021 tgccagaaca accggtactt ctcctaccgg gcggagcagg gattcacggg gcggatgctc
     1081 tcggtggtag gaatccgaag gtaggccggc gccccccggc gatgggctgc actcgcttcg
     1141 gtcggaatga catccgggct tcgtttcgcc agtggcttgt tgtccatgac ggcgcgctga
     1201 agtttccgga tccctggtcg ccatgcgtgc cggccaaacg ttctgcgggc aacccgctgg
     1261 aagagccagc cttccaatgc tcgcgttcat ccgcagggag ttatgagcaa atccaggata
     1321 gagaatcaga agcacgccac ggacaaggtg agcaagattt cgtctgagat tgccgctcaa
     1381 accaggggcg acgcgtttgg gaagttcgcc gcggccgctt ccgcatggct gggatcgaag
     1441 tgggctttcc tgggcgcgat ttcgatcatc gcggcgtggg cgattaccgg ccccatcttc
     1501 cacttttccg acacctggca gctgattatc aataccggca ccaccatcgt gacctttttg
     1561 atggtgtttc tgattcagaa cacgcagaac cgggacgccc gcgccatcaa tctgaaactc
     1621 aacgagctga tccataccag tgccgcggcc ggcgatcaga tgatggatat cgagaagttg
     1681 agcgacgagg aactggacgc tctgcaggcg cgatacgaaa agatccgcgc ggagtggatg
     1741 gagcgaaaca agggtcaggg cggggacaag agttagccta tcgcctggcg cgagcggctg
     1801 gcgacgcgtc cgtaatacct cgtattattg gcgaccggaa tcgcttccgc gcctgcttag
     1861 gttgggggta tagctttacc tcgtttatgc ccccgtaggt acttcgcgcc agccagacgt
     1921 tacatcacca ttcccggctg atggcggatg ctcagcactg gcactttggc tcgcgcgatg
     1981 atgctgtaca ccgtgtcgtc gccgcgaatc ggccagaatg agacgttggc atcgaccccg
     2041 agcatgatca ggtcggcact catctccgca gcgacgttca gcacttcgtc cacgacatcg
     2101 ccgatctcca cctgaaccga cgggtgggtc cacagaggcg cctcgtcggg aatgacccgc
     2161 ttcaattccg atcgggtcca ctccaccact cggtccgcat ccggctggct gttcacgctg
     2221 cgcgacagca catgcagcag cgtgatgtcg gcccggtaaa actccgccat ctcgatggcc
     2281 atgcgcgcgc tgctctcgta tccggcgcag agagaaaccg ggtgcagaat cttctgcggc
     2341 gcgccgaagg aggacgcttc gcgggcgtgg ggtccgatgg tgcagacggg cacctcggcg
     2401 gaacggagaa tttcgtgtgc caccgatccg agcaggagct tcttgaggtg acggcggccg
     2461 tgggttccgg caatgatgcg accagcgtgg agttcgcgag ccagcgtggt gatctgctca
     2521 cgcggattgc cctcggtgac caggatgtcg cactcgatgc cggcagcctg ggccttttcc
     2581 gccatgcgct tcagggtttg ctccgcttcc gctttcattt cagcgacatc cagatagggg
     2641 atggcagcgg cgtcgagtgg aacggcctgg ccgggcggga tgacgtgcag cagggtgagc
     2701 gtgctgccgc aggcccgggc ctgcgcgatg gcatggggga gaagatattc gctgtcttcc
     2761 agatccgttg caacgagaat tttttcgggg accacaaaac tgcgcttgct ggaaaccgct
     2821 tcgtcgaggg ttttcatttt gaaaaacctc cctgccggaa agtctcccgc caccttatgg
     2881 atgtggctgt gattgcaggc accagcccct gtgacggggg tcacgagccg atccgatctt
     2941 cctgttgctt aatgtcagaa gccgaggcgt aaccagggag acagcgcccg gtgatagcag
     3001 tcacatacaa ctgtttttcc tcagagatac ggtctgtctg catttctcca ggctttgaag
     3061 agcggacagg agggccggat ggccacagaa gaatcggttc tggaaacgtg taaaaaacgg
     3121 ggtatttcgc gcaggcggtt catggagttc tgcggcacga tggctgcgac cctggcgctt
     3181 cccaaaggct atggacagac catcgccggc gcgctaaccg cgaaacgcaa acccacgctg
     3241 gtgtggctgg aattccagga ctgcgccggc aactccgaag cgatgctgcg gtcgacgcac
     3301 ccgacggtcg aggaagttgt cctggagacg ctctcctggg agtatcacga gacgatcatg
     3361 gccgggtacg gcaagcacgc ggaggcggcc ttgcgtcgcg tggtggaaga agagaagggc
     3421 aagtacctgg ttgtcgtcga aggctcgatt ccggccggag acgatggcgt ctactgcacg
     3481 attggcggac gcacggccct cgatattgcg cgcgaagtct gcacgaacgc cgcggcctcg
     3541 atcgccatcg gctcctgcgc gtgggatggc ggcttgccga aggcgaatcc gaatccgacc
     3601 ggagcgatgg ggctgcacga ggcggttccg gggattacgg tggtgaacct gcctggatgt
     3661 ccccataatc cgacgaacac ggcggcggtg ctggtgcatt acctgacgtt tggcgtgctg
     3721 ccggccatgg atggctacaa tcgcccgctg ttcgcctatg ggcagctgat tcacgatcaa
     3781 tgcgaacggc gcgcgcatta cgacgccggc cgctatgtgc aggcgtgggg cgatgagggc
     3841 catcgcaagg gctggtgcct gttcaagatg ggttgcaagg gaccggaggc gacctacaac
     3901 tgccccacgg ttcgattcaa cgactcgacg agctggccgg tgaagtcggg ccatgggtgc
     3961 atcggatgcg cttcgtcgcg cttctgggat accaagtcac cgttctacga gcggctcccg
     4021 catccggcgg gcttcggtgt ggatgtcacc gccgggcaaa ttggctggac ggtggtgggc
     4081 gccgtcgccg cagcgaccgc cgctcatgcg gttggacacg tcgtgcgcga ccatttccat
     4141 ccgagcggcg agggcgcggc caaagaagaa acagcggaaa aaaccacgac gggggtgaac
     4201 tgaaatggca cgagtcgtca tcgatcccgt cactcgcatt gaaggccatc tccgcattga
     4261 gacggaggtg aacggcggca aagttacgga tgcctggagt tcgggcacca tgttccgcgg
     4321 cattgaaatc atcctgcgcg ggcgcgatcc gcgcgaagcg tggatctggg cgcagcgcat
     4381 ttgcggcgtt tgcaccacgg tgcacgcgct tgcctccgtt cgcgcggtcg agaacgcgct
     4441 cggcatcgag gttcccgaca atgcacgttt ggttcgcaac atcatcgcgg gcgcgcagaa
     4501 cgtccacgat cacgtagtgc acttctatca ccttcacgcg ctcgactggg tcgatgtgac
     4561 cttagccttg aaggccgatc cggcgaagac ttcgcaactg gcgcagtcga tctcggaatg
     4621 gccgaagtcg agcacaacgt atttcaaggg agtccaggac cgcgtcaagg cgctggtcgc
     4681 cagccggcag ttgagcctct ttagcagcgg tgactgggga caccctgctt accaactgcc
     4741 gccggaagcg aacctgcttg ccgtcgcgca ttacatcgaa gcgctggaca tgcagcggga
     4801 ctttattcgc atccacgcgg tcctgggcgg taagaatcca cacctgcaga cctatctggt
     4861 gggtgggatg tctaccgcaa tcgatggcaa cgagccggaa gccgtcatta atccggaacg
     4921 tatcacgctc tttaatgagc ttgcggctcg tgccaaaacc ttcgttcagc aggtttatgt
     4981 tcccgatgtc ctcgcggtcg ccagcttcta caagcagtgg ttcggttacg gcgagggcct
     5041 cggcaatttc atggcctacg gcgatttgcc ctcgggcagc gtcaacgatc cggcgggctt
     5101 cttcttcccg cgcggcgtga ttttgggccg cgacctgtca acggtgcatc ctgtcgatcc
     5161 gggtaaggtg tcggagtacg tgacccactc ctggtacgac tactcgcaag gcgaccaggt
     5221 gggccgccat ccatcgcagg gcgagaccaa cccgagatac tcggggccga agccgccgta
     5281 cctgacgctc gacacgaacg cgaagtattc ctggctcaag tcgccgcggt atgacgacaa
     5341 gccgatggaa gtgggtccgc tggcgcgcac gctgattgcc tacgcttccg gcgatcccca
     5401 ggtgaagaag acggtggatg cggtgctggc aaagttcaat gcaccgccca cggcgctcta
     5461 ctcgacgctc ggccgcatcg cggcccgggc gatcgaggcg gacctcgtcg tgcagaagct
     5521 gccggagtgg atcgcacagc tcgacaacaa tatggcccat ggggatgtgc gtatccacaa
     5581 cggcgacaag tgggatcccg acacctggcc gaagtcctgc gccggatttg ggctcgaaga
     5641 agccccgcgc ggttcgctgg gacactgggt tgagatcgag aacaaaaaaa tcgtcaacta
     5701 tcaggccatc gttccttcca cctggaatgc aggtccgcgc gatgcgcatg ggcagcgtgg
     5761 agcctacgag gctgcgctgc tcaacacgcc gatcgccgat ccggagcgtc cgctggaagt
     5821 gcttcgtacc gtgcactcgt tcgatccatg cctggcttgc gccgtgcatg tggtcgatgg
     5881 aaagggccgc agttacggca gggaagtcgt ggctgtagcg gaaccggagg gatccatgtc
     5941 gctctctcct gttccagcgg ctctgtgttc cataggaggc gagtgatggc cactctggtt
     6001 cgtcccgcgc cgaagaagca tgtggcggcg ggcgaagagc acctcgtccc cgtctacgtc
     6061 tgggagctgc cggtccgctt gagccactgg ggcatcgtgg ccgcggtcac ggtgctcacc
     6121 tggacgggcc tctacatgaa ccatccgttt ctcatctttc acggcccgcg cgagtggacc
     6181 atgggaacgg tgcggttcat ccacgagctg gccgggtttg agctgatcgg ggcgctgctg
     6241 gttcgcatct actggttctt cgccggcaat cactgggcgc gctggcggca gttcctgccg
     6301 ctgacccagc ggcagtggaa gagcctgaag gccatgttcc gttactacac gttccagcag
     6361 aagaagccgt tcgaacagat cgggcacaac acgctggccg ggctaacgta catcatcgtt
     6421 tacttcctga ttgcctgcga gtgcgtcacg gggctcgttc tgctggcgac cgtggggggc
     6481 tcgcacacac taacgttctt cgttggctgg attccccaca tcatcgacat tcagtggctt
     6541 cgcttcgtgc acttcctgtt catgttcttc ctcatgggat tcttcattca ccacgtctac
     6601 agtgcgacgc tggtggccat gaccgaacgc aacgcggaga tggaaagtat cttcagcggt
     6661 tacaaattcg tggatgacca gacgattgac gaagagctgg agatcgcgcg actggagccg
     6721 caggcacttt tcagcaagcg aaaaatccat cggacgaggg attgggatcg atgagcactc
     6781 cccgcatcgc cgttctggga cttggcaatc tgatgcggac cgacgatgcg gtcggcatgc
     6841 tggccctgca acgcctgcgt tccgaccctc gttttccgca gtcgattccg ctcatcgagg
     6901 gcggcaccct gggactggac ctgatctatc cgctcgacgg tttcacgcac gtgctcgcgc
     6961 tggacgcgat cgatgccgga gttcagcccg gcacagtgct gcgttattcg ggggatgcga
     7021 tcgccgatct gcccatctcg aagagcgtcc atctgctggg attctcggat ctgatcggct
     7081 cgatgcggct gatcggcaat gctcccgagg aaatcgtcgt cctcggagtg cagccgaaga
     7141 cgatcgactg gggaacagaa ctgacctcgc cggttgaggc cgcactggat attctcatcg
     7201 agcaggttct ggaccagatc ggccagtggt ccgaagaaaa tgagggaatg ccggccgtgt
     7261 cgaaccgggt cgaggcgact gtaccgcagt tggcagccgc tgaagaagcc tggtattggt
     7321 agattggcct gccataggca gggaaattgg gccgcgttac attgcccccc cagagggatc
     7381 atgaactgca cacgcatcat tacccgccgg agcctgtccg atgcatgaag tcggcatcgc
     7441 gacgtccatt ctctcggcgg gccaccgcga gacagaacgc cggcccggag ccaggctcgt
     7501 cggcattggc gtgcgcatcg gagtgctgtc cggcgtcgat gtcgacgcgc tgcgttttgc
     7561 attcgagtgc atcacggccg aaactgaaga tgagaaggtg gtgttgacca ccgagcaatg
     7621 cccgcgggtc aatcgctgtg aggactgcag gcacgaattc tcgtcccggc aatcgtcgcc
     7681 gttctccgac gccccctgtc cgcgctgcca cagcgcccgc accagcttcg tcagcggcga
     7741 tcagctggat atcgcgttta tcgaagtgga ggaaccatga ccgttgtccg agtcgagcag
     7801 aaagtcctca atgagaatca gcgcatcgcc ctcgagctgc gccgccgctt cgcggaacac
     7861 gacatcctct gcctgaatct catcagctcg ccgggttccg gcaaaactac gctgctcgag
     7921 cagacgcttg ccggtcttgg gccggaggag aaaatcgcgg ttcttaccgg cgatctgcaa
     7981 acggagaatg acgccaaacg cctggcgcgc tacggttttc ccgtgcagca gatcgtcacc
     8041 ggcggtgtct gccatctcga cggcaagatg atcgagcgcc atctccaggc ctggtcgctg
     8101 gaagacatcg acgtgttgtt catcgaaaat gtcggcaatc tggtctgccc ttcgagttac
     8161 gacctcggcg aagaggcgaa gattgtcctg ctcagtgtga ccgaaggcga agacaagccc
     8221 ctcaaatatc cgtcgatttt cctgaagtcg tcgttgctcc ttctcaccaa ggtcgacctg
     8281 ctgccctacg tccccttcga catcgaggcg gcaaaggcaa acgcgcggca aatcaacccc
     8341 gcgatcgaga tcctcgaagt ctcgtgctcc accggcaagg gcatggacga atggcggacc
     8401 tggctccgga atcgacgtgc ccggtctgct gcgacccctg caaacgggcg atagccggca
     8461 gtcgagccct tccggttcca ccaaaataat atttcttcga accaggagta atgactccgc
     8521 tgggcagacg cgtccccgcc gcacggggcc tgatcgagcc tcacagcccc attctgcagg
     8581 acttagcagc accctgaatc gggaaacccc tcaccggccc gttccgaacc gggtatgagc
     8641 taagtgatac aaatcactgc gccagaagta accccgacct gtgattctca tcactgtgtc
     8701 gcatttctga acaccgctat tctccacgag tactgaaccg taatttcagc aaaccggggt
     8761 cgcaaggctc cgcttgctgt gcgaggtgaa tacgcatgcg ccgtttccta ttcagcatcg
     8821 cgtgtcttct gctactgacc gggttcgcag gaaccaggct ggcgctcgct gccggcggtg
     8881 gccagcagcc tgcggcaact tcccaatcga aggcggcggt agactcgacg aatttcgtcg
     8941 gtcaggagac ctgcgccact tgtcatgagg acgtggtcaa gaaatttgga acgaatcctc
     9001 actctcgtct cgccctcgaa catgccggca agggcgttac ctgcgagagc tgccacggtc
     9061 ccggccaggc gcacgttgaa ggcggcggcg acgtcacgaa gatcaggcgc ttcgacaagc
     9121 tcggcaccaa acagatcgac gcaacctgcc tgggctgcca cgccggcgcg catccgaact
     9181 tcgaccgctc tccgcatgcg aaggcggatg tgagctgcct cgggtgccac aacgtgcatg
     9241 attcgaaaac ccccgagcat ctgctgaaag ccgaacagcc gaagctttgc ttccagtgcc
     9301 acaccgatca gaaggcgaac ttcgacatgc ccttccatca cccggtgaac gaaggcacag
     9361 tgagctgcag cgactgccac aatccgcacg gcacgttcaa caaagcgaat ctgaaggcga
     9421 tcgccgatca gaacgccgtc tgcaccaaat gccacacgga tactcgcggg ccgttcgtct
     9481 tcgagcacgc cgcggtcaag gcagagggct gcacgggttg ccacacgccc cacggttcgc
     9541 agaatgcgcg aatgctgaac atgccgtcga tcaacatgct ctgcaaccag tgccacagtc
     9601 cggttgccga ggcgacggtc cacgggatgg gtgcaggctc gaccgcatcg cagccctgca
     9661 tcagctgcca caccatgatt cacggatcga acgtcagtca ggcgttcatc cggtaacggt
     9721 tcaagcgagg cttttatgaa gacgacaggc tggttttcct ggctctccaa tccgcggcgc
     9781 tcacggactc tcgccggccg cgtgggtcag ggtgttggag ttgccatcat cggtatgacc
     9841 acgggcgtcg ctcttgccca ggctcccgcg ccggacacgc agccggtggc gccgaacggt
     9901 tacacgctgc accaaagcgt ggatctcggc ggccgcatga ccaatgtgac cggaagcggc
     9961 gccatgtacg acaccctggt caacatgcgc tcaggcccgc gcgtgctggg cgaaaccttt
    10021 gagctccggg ctctgcccgg cacaaagcat atgctggtgg actcgctgac ggcgttcggc
    10081 agcggattcg gcggcgatcc caacaacttc gctcgcctta gcttctccaa aggcaacctg
    10141 ttcgattttt ccggactctt tcgtcgcgat cgccagtact tcgactacga tctgctcggc
    10201 aatccgaata tcgttcccgg aaggtcgatt ccgatcggcc caacgagtgc tccgacgggt
    10261 tcgctcgcct ggccccaggt gaaggtatcg ccggtcatgt tcaacaccgt ccgccgcatg
    10321 acggatacaa accttacgat cctgccgctg tccacgttca gctaccggat cgcctattcg
    10381 cacaatacat tcgagggacc ggctctgagc cccagctacg cgatcgccaa atacgacgcg
    10441 ctgctgcggc agtatcagcg caacggctcg gacgattaca ccgccgccct ggactggaaa
    10501 ccgctgcccc aaacccgggt gactattgaa gaaaacgtca accgctacaa gtccgacacc
    10561 tttttcactc tgaatccgaa tcagttcatg gtgcaggagg cagacggaac aaaggtggat
    10621 ctcggcaact gggacagcga gacgccgtat ggcgcctccg cctgcaacgc gaacagcctc
    10681 gggaccactc ccgtactgtc ggcgccgcag actccgggcg gtctgccggt gatcaatgcc
    10741 gcctgcgcgg tcgtaaccag ctatttgcgg acgcagccaa cgcgcatcac gattcccacc
    10801 tcgatcatcc ggctgcaaag ctccagcatt gcgaaggtca ccatgaacgg cgacttccgc
    10861 tataccctgg ccaatatgga gatgccgaat tactacgaga acttcaaggg tctcaacggt
    10921 gccgttcgtt ccagtaccta cagcggcggt tccgcgagcg cacaccgggc cgtcctttcg
    10981 gccgactacg ggatcgtgtg gcaggccacg gacgcgatca gcatctcgga tcagtttgac
    11041 tactccagcg ttcagcaacc gggctggtcg aacattccgg cgcctgcgac gctttctact
    11101 cccaccacgg cgggcaatca gacaatcaac tactccggcc cgctgacgcc cggaaccggt
    11161 gcgctgccgc acgggatcga gggctttcgt tatcccggat tcttcgggca gtcgtacatc
    11221 accaacagcg caactctcag ctgggacgcg acgccacgga cccggtactt catcacctat
    11281 cgctacagcg atcacaccat cggcgagggt ggggatcaca cagtacgtcc ggtggataca
    11341 tcggtcgacc cgttccaggg aacggtcgag atcaaagaga acgccggcat ccttggcgtg
    11401 agcctgcggc cggcgacaaa ctgggacatc aacggctcgg ccgagatcgg ctacctcgac
    11461 aacgcgttca ctccggtagg atcgcgccag ttgcagcggt acagggttca caccacctac
    11521 aagccgaaga gatggatggt gctgtcgggg gccttcaacg atctcgagcg ccataacaat
    11581 acgttcaaca acgaagaaga gatcgcggcc ggagcaatct actacggtcc catcaatcac
    11641 atcgatcaca cccgcattgc cagcctggga gcgactctga acccaaatga gcactacggg
    11701 ttcgacatga actatgtctg gaacgatgtc tatacagcga ccaatacctg cttcaccagc
    11761 ggcgctgcgg ccaaccttcc gggaacggcc acgctgaaga gcgatggcac gccggacgtt
    11821 tgcccagggg tcttcgcgcg cggctcgacg accattctgg tcgactggca tgcccgggcc
    11881 tacgaggaca taccggtgca gtatgggtcg gcgtcgattc acatcactcc gaacaacaaa
    11941 ttccgctccg acatcggcta tcgcatcgat tcgtcgaacg gaagccgctt ctatcaggat
    12001 ccgcgcgatg tggccggatc cctggtttcc acctggcagc agcccttcgt ggatgttgcc
    12061 tacacgcttc atccaggatt catctggaag gccgagtaca acttcttcgg ctacggcgag
    12121 ggcggaccct ccggaccggc ctactgcagc ctctccacag gactgacggc ctccgttgtg
    12181 ccctgtgcat cgctgccgta tccaaccggt ttgaccgagg cgtcttccgg tcttaccgcg
    12241 ccgcggaatt tccacgctaa caatgtcacc ctcggattcc actacgagtt ctgaggctct
    12301 tcacgatgaa ccatggccgg aggctcaagc ctccggcccc agcagcaagg agcgaaaggt
    12361 gaaacagaac gtccgtattg ccgtcctgac tctgctgagt gcttctcttg tccttcccgc
    12421 ggtcgcgcaa caggccggtg aggcgactta caaagcgaaa tgcgccatgt gccacggcgc
    12481 cgacggggtc ggcaacacaa cggttggcaa aaatatgaag gtgcgctcgc tgaagtctcc
    12541 tgaagacatc aaggcgaccg atgccgagct cttcaagcag accaaagaag gtgtcggcaa
    12601 gatgcagggt tatgccggca agctgaccga cgcccagatc agggacgtgg tcagcttcat
    12661 acgtacgctg caaaagtaag cgagtacgga acagggaaag ggcgaggccg gcatcgacca
    12721 gcgattgccg gaccacgctg gagttgtgcc cggcctgagc atgaaggtgt tccggtccgg
    12781 ggattccgcc gggcgcttcc cagctacccc gtctggaatc gggaaagccg aaggaatggg
    12841 gacccaagcg gtaaatctgc ctgcagaatg tcgttccaaa ggcagccggg ctcatcgaca
    12901 tcttctcttt gacggtctcg ttcttctctt tttcattact gcgctgtcgg ttctccctca
    12961 ggcacgcgcc cagaaggcat cggattgcct gctgtgccac ggtccttcaa caggactcaa
    13021 aaactccaaa ggcaaaagca tcaccatcag cccctcgacg cacatgaagg gcccgcactc
    13081 cgacctcggc tgcctggatt gccataccgg cgcctcagaa cagacgcaca acgcgaaaag
    13141 cgcctcagct tcgtgccaga cgtgccacgc cgattcggcg aaagccatga ccacgggcgc
    13201 tcacaccgct ctcggcaatc ccaacgacgc gcagacctgc atcacttgcc acggcaccac
    13261 caacacggcc aaagctgtcg ccggacccca gttttgcgcg acgtgccacg cgagcgaagt
    13321 tgaacaatac cgctccagcg tccatggccg agcccaggcc aaaggcaatg ccgatgttcc
    13381 cacctgtcaa agctgccatg gttctgcgca cgccgcgctg cctgcgaccg acccgcagtc
    13441 gccggtgagc aagcagcacc tgccggatac ctgcggctcc tgccattcca acccgcaact
    13501 ggcggcgaaa tatatgttcg ccgaggtgcg gccggtccag gcctatcgcc aaagtgtgca
    13561 tgggcgcgcg ctggagcacg gcaacaccag tgttgcctcc tgcggcgatt gtcacggaac
    13621 tcacgacatc ctgccggcct ccgatccgaa gtcgaagatc tggaaacaga acgtcgcctc
    13681 cacctgcggc aactgccaca ccggcgtctt caatacctat gcgcagagca ttcacggcca
    13741 ggcggtgtcg aaaggcgtgc tgcaggcggc cacctgcacg gattgccaca gcgagcaccg
    13801 gatcctcgcg ccgggcgacc ccggttcgcc ggtctacatg gcgaatgtct cgcaggaggc
    13861 ctgttcgcgc tgccacgccg atacgcagct catggccggc ttcgccatgc cccaggatcg
    13921 cgtccccaca tatgaggaca gctaccacgg cctggccgcg catgaaggcc gccagacggt
    13981 tgccaactgc gccagttgcc atggcgtgca caacatctat ccatcgagcg atgcgcgctc
    14041 cacggtgaac cacgccaatc tcagcaagac ctgcggccag tgccacgaag acgcggggac
    14101 ccggtttgcg atcgggccgg tacacactct ctcgaagacc actcccggcg gccgcctcct
    14161 tgacttcgtc aaaatcttct acttcatcgt cattcccgtc acgatcgggt tcatgctcct
    14221 ccacaacctg atcgatctgc ggcgcaaagc gcgtaaaatc ctcgccggct atcgccagca
    14281 ccctggccag attcgtctca cgctcagcga gcgctggcag cacgtgctgc tggccgtcag
    14341 cttcatcatc ctggtcatca ccggctttgc tcttaagtat ccgcatgcgt tctgggtcaa
    14401 gcccatcgtt ggctgggagc accacatgcc cattcgggga tggatccatc gcatcgccgg
    14461 cgtcgttctg atcggcgcct cggtgtatca catggtgtat ctggctgcga aaaagagcgg
    14521 gcggcgctgg ctcaaagaca tgatcccgga tctccgcgat gccaaagagg cgatcggaac
    14581 ggttcagtac aacctcggcc atcgcgatca gcttccccgc tatcgccgct tcaactacgc
    14641 cgaaaaggcc gagtactggg ccctggtctg gggaaccatc gtcatggcca ttacaggcgt
    14701 gctgctctgg ctcaacgact ggatcctcgc ccatctcccg cacccggcga ccatcctcgc
    14761 tgtcagcacc gcgattcact tctacgaagc aatcctcgcc actctggcca tcgtcgtctg
    14821 gcacttctat gcggtgatct tcgatccgga gatctatccc atcaagtgga cgctgctgac
    14881 tggccgcgct ccggagcacg aagtgcgcga ggtcgaggac gaacccgctt ccgaaaagaa
    14941 attgtctgac ggttcgggcc cggcgcagat gccgcatccc cagtcggagg gcgacgactg
    15001 atcctcgtgt gccaactttc accgcacccg agctaagata ttcccgctcg gtctgtggcc
    15061 catatcacag accgggcaac ttcccgcgcg gtatcaacgg gaaggacttg ggacccaagt
    15121 accgctgaag gagctgaaaa atgaatcgca tcagaaccgc ggccatgatc ctgctcgccg
    15181 cctccattgc ggcgcctgcg tttgcgcaga gcgccggagc cgacacctac aaagccaaat
    15241 gcgccatgtg ccatgggccc gatggcaccg cgtccacgcc ggcgggcaag gcgatgaaga
    15301 ctccgtcttt caaggacccc gcctcggtca agtccaccga tgccgatctg atcgcggtca
    15361 cgaaaaacgg caaaggcaaa atgcccgcat acgccggcaa actgaccgac gcccagatca
    15421 aagaagtcgt ctcgtacatc cgcactctgc agaaatagcg gaaaacggga gagtttggcc
    15481 gttcgtgatg cggtgagaac agggaagagg cgcctgccat tcgcctcttc cattcgccgc
    15541 aatcggccgg ccaggaacca cgggagctgc aatgaggacc ctttcgtctt ttggcctggc
    15601 aatttttgcg ccagcgctgc ttttgatcgc ccttacccgg cccctgtacg ccgatgtcga
    15661 ctgtctttcc tgccacagcg acaccacgat gcaggacgcc aacggcaaaa atgtcggcgt
    15721 cgacgccaat aagttccacg cgggcgttca cggcatcctc aaatgcggcg attgccacaa
    15781 agacattcac gactatccgc atccggctca accagccaag gtccagtgcg aaacctgcca
    15841 tgccgatgag gcctccgccc tggccggcag cgttcactcg aataagaacg agcatccctg
    15901 caccagctgc cacggcaatg cccacgaaat tgtcacaaag acggatccaa attccgcggt
    15961 ttacccgctc aacattccca ggacctgcgg ctcctgtcac ggcaatccgg ccatggcgca
    16021 gaaatatcat ctgcccaacg tctacgccct ctacatggac tccattcacg gcttcgccct
    16081 cggcaaggag ggcctgctgg tcgccgccaa ctgcacaagt tgccacggct cgcataagat
    16141 cctcccgcat accgacccgc agagccgcac caatcgcgcc aatatcgctg caacctgcgc
    16201 atcctgtcac ttcggcatca acgagcagta tcagcagggg gttcacggca aggcggtcgc
    16261 ggcgggcgac aaacatgctc ccgtctgcac tgactgccac acagcgcacg ggattgtcga
    16321 gcccacgtcg gccgacttcc gcatgcagtc cacgcccatc tgcggatcct gccatcgtga
    16381 gaagttctcg acgtatcgcg atacgttcca ttcgcaactg ggactgctcg gcggttatgt
    16441 ggagacggcc cgctgctggg actgccatca ggcgcacgac gtccgcccag cctccgatcc
    16501 caactcgccc atcgccccgg cgcacctggt ccagacctgc ggtcgctgcc atgcgggggc
    16561 caacgccagt ttcgtgcaat accagcccca cgccaacgca cggaatcgca agctgaaccc
    16621 ggcgctctac tacatccgcc tgttcatgaa cctgctgctc accagcgtgc tgacgttctt
    16681 taccctgcac actgtgctgt ggctgatccg ctcgcgcttc aagggcaacg gcaagttgcc
    16741 gggagggaaa aatgcctgaa ccaaccgccg cccccgcagc tgctgacgcc gtcccggcgc
    16801 cggcagtcga ggctgtcatt tccccggccg ctccggaaaa agagcgctgg ttcctgcgct
    16861 tcaccctggc gcagcgctac ctgcacgccg ttctgttcac gagcttcctg gtgctcgccg
    16921 cgaccgggct catgatgcgc ttcagcggat cgccctgggc cattcgcttc gcgaagctcg
    16981 tcgacggttt cggcaccatc ctcttctttc ataagttctg tgcactcgtg ctcagcctgg
    17041 cgtttgtgat ccacgtgcgt gagatcctgg cgcgtggcgt gttcaaacgc gaaaaaggca
    17101 tcttctgggg accgacgtcg atggtggcca actggaaaga cgtcaaagat cttttcggac
    17161 acatgcgctg gttcctcggc ctcggcccca agccgaaatt cgaacgctac gcctactggg
    17221 agaagttcga ctactgggcc gtcttctggg gcatggtggt catcggcttt tccggatacg
    17281 ccatgtggtt cgcgcccttc ttcgcgcgct tcctgcccgg ttgggcgctc aacgcggcgc
    17341 tcgttatcca tagcgaggaa ggcctgctcg ccatcctctt catcttttcc atccatttcg
    17401 tgaacaccca cctgcgtcct gacagtttcc cgatggacat ggtgatcttc acgggccgcg
    17461 agagcgagga ggaatttaaa catcggcgtc ccgacgaata ccagcgcatg gtcgccgagg
    17521 gaaaagctgc gacaaggatc acagacgcgc cccctaagtg gttgataaat ttcagtcggg
    17581 tggtcgggtt taccgctata ttgattggcc tgactctcct cgtgctgacg ctgagcgcgt
    17641 acttcgctga ataagaggtc caggcaatgt cccaatggcg cagactgaag gaagactggc
    17701 tccagccgtt tttcttctat ggcaacaacc ttcttagcct gattggcggt gcgctcacca
    17761 ccgcggcggg ctgcgtcctc gttggcttct ggatcgtctc catcttcggc cacgccggtt
    17821 cttccaatcc ttacctcggc ctcattctcg atcttttcct gccgggtttg tttgtcttcg
    17881 gccttgctct cattcctgtt ggcatttggc tgcaccgcag gcgcctgatc gctgccggac
    17941 aagtacctgc cacctacccg accatcgatc tgcgcaatcc cgccttccgg catggaatag
    18001 aagttgtcgt catcgcgacg ttcatcaact ttgtcatcgt cggcacggcc acctatcgcg
    18061 gcgtctcgta catggattca ccccatttct gcgggcagtc ctgccatgtg atggcgccgc
    18121 agtgggccgc ctttcaggaa tcgccccact ccagcgtcga ctgcgtccag tgccatgttg
    18181 gctcgggcat gaagtcgtat atccatgcca aggtgaacgg caccaaacag ctggtcgagg
    18241 tcacctttca cacctggccc acgcccattc gcgccaatct caataccctg cggccggcgc
    18301 gcgaaacctg cgaggcttgt cactcgccca cgcgcttcgt cggcgagaag ctgctggtca
    18361 agaccacctt cggcgacgac gaaaagaacg cgatgacccg caccgttctg gttttgcatc
    18421 tgggcggtgt cgattcggtc tctcacttca ccggtatcca cggccatcac ctgaacaact
    18481 tcgaatacgt ggccactgac aacacgctgc agaccatcct gtcggtcacc gccaccaatt
    18541 ccgacggatc gaagaccgaa tacatcagct ccgacgccaa aggccccatc cagggcgtgc
    18601 gccgcaccat ggactgcatc gactgccaca accaggccac gcatgtcttc cagacagccg
    18661 aggaagccat cgatcgcgcc atgctggatg gcacgccgga cccgaacctg ccgttcattc
    18721 acaagcaggg tctccagctc attcaggcga cgtattcatc gcaggccgaa gcgggacaga
    18781 agatcacctc aggcctcgaa gacttctacc ggacgcaata cccgcaaatc tggtcctcgc
    18841 agcgggcgca gatcgaggca tcagcgcgtc ggctggttgg aatctacgac cgcaacgtct
    18901 ttccgtcgat gaagattacc tggggcacgt atcccaacaa catcggccac atggcctatc
    18961 cgggctgctt ccgctgccac gacggcagcc acaccagcaa
//
LOCUS       DASZYN010000648        18452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_291116, whole genome shotgun sequence.
ACCESSION   DASZYN010000648 DASZYN010000000
VERSION     DASZYN010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 18452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18452
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291116"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VHE33_16700"
     CDS             <1..907
                     /locus_tag="VHE33_16700"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT99147.1"
                     /translation="LPAVEAFRIEYWLLEEAKLRRQPAEKELSRRIALIVGGASGIGR
                     AAALIAAERGAHLMIADRDLAGAEAVAQELRASAGKETIAATAVDIRDRSSIAKALRE
                     TIARFGGLDILINTAAIFPSSPQGAISDAMWSTTLDLNVTANYLLAEEAARIFNTQKI
                     DGSIVLTSSANAVVPKRGSEAYDVSKAALSHLIRELAVSLAPHVRVNGISPATVVKGS
                     TMFPRDRVITSLRKYDIGFEPSMTDDQLRDLLAGFYAHRTLTHQPIEPEDCARAILFL
                     AGPHTRCTTGHLIPVDGGLPEAFLR"
     gene            904..2334
                     /locus_tag="VHE33_16705"
     CDS             904..2334
                     /locus_tag="VHE33_16705"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HVT99148.1"
                     /translation="MSYFPALVAIDLGAESCRVSLLRWRGGEASITMARRFENGPQHH
                     GAAGWHWNLDRICNELEAGLRTCAEWSPEGIAAIGVTGWAVDYVRLDPFGRPLTAPFC
                     YRDARNQRAMAEVHAAVPAEKLYARTGVQIQPINTIYQLYADRSSGIPDSAPWVNLPE
                     YILHWLGAPRVAEYTNATHTGLIDGDTRQWSGEIFAALSLDRAAAPEIVAPGTTLGPV
                     RDDLRRLPAFAQTQLIAPACHDTASAIAGIPERRGDWAYISSGTWSLVGMPLTQSLRT
                     QEAFSRGFTNLGAADGGTLFHRGLPGMWLLRQCMNTWDQERGWQFGDLIAEASRMPAP
                     DRLLDLDDPALLPPGDMTARINEQRRRLGSPSLPSGCDAAPHYASLIFRSLAARYGTL
                     IAEIAEMTGEKPHHIAVVGGGSQNEYLNALTAEATGLPVQRCSAESSTLGNFAIQWAR
                     IEQSSGTLQPQSVAEKVSALAAVQVA"
     gene            2352..3386
                     /gene="rhaT"
                     /locus_tag="VHE33_16710"
     CDS             2352..3386
                     /gene="rhaT"
                     /locus_tag="VHE33_16710"
                     /inference="COORDINATES: protein motif:HMM:NF010023.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HVT99149.1"
                     /translation="MSTAILGGIGWHMVGAAAAASFYAPIEKVKKWSWETTWAVAGMF
                     SWILLPIIVSLVLLPDFHAFYASIPSDVLLKTFLFGCMWGVGNVNYGLTMRYLGMSLG
                     IGVAIGVTLVVGTLVPPIVHGQLGYLITSRSGHFTLSGIVLALIGIATVSWAGHQKEQ
                     RMGIRTEEFNLTKGLLLAVMCGIFSSGMSFAIDAARPIASAAQLAGVKPLYAALPSYV
                     IIMGGGAIVNFAYCFSRLAFRRDLSLRSDLSQTKRTLVLNSSLAATAGIMWYLQFFFY
                     AWGAANIPVHLSFVNWMLHMSMYVLFGGIVGLALGEWRGARGRPVQLLWSGMVIIIAA
                     ANLVGLGMAT"
     gene            3481..5601
                     /gene="glgX"
                     /locus_tag="VHE33_16715"
     CDS             3481..5601
                     /gene="glgX"
                     /locus_tag="VHE33_16715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HVT99150.1"
                     /translation="MHRTLLPGKPYPLGATPTSIGTNFALFSENATGVSVCFFDENNN
                     QIDCVALRERTAFTWHGLVRGIKPGQRYGYRVEGPWEPEKGLRFNSNKLLVDPYAKAI
                     SGEVDWKAPIFPYDVTTGNDLTRDDRDSAAGVPKSVVMDPHFDWEGDCPPEIPLADSI
                     IYEVHVRGFSKLNEAVPENLRGTYAGLACEPSIEYLKKLGITAVELLPVHHFIDEGHL
                     VDRGLRDYWGYNTLGYFAPMSRYSSSGDMGQQVAEFRQMVKALHKAGIEVILDVVYNH
                     TCEGNRMGPILSMKGIDNTTYYRQVRHDPRYYMDYTGTGNTLNVMNPQVLKLVMDSLR
                     YWVTEMHVDGFRFDLASTLARELHAVSKLSSFFDTIHQDPTIADVKLIAEPWDVGEGG
                     YQVGNFPVLWAEWNGRYRDTVRRFWKGDSGLLSDFAYRLTGSSDLYEMDGRRPNASIN
                     FVTAHDGFTLCDLVSYNHKHNEANGDNNRDGTDDNHSWNMGAEGPTDDPAINTLRERQ
                     MRNFLATLILSQGVPMLCGGDELARSQKGNNNVYCQDNELAWFNWKLTPAEDRLRDFS
                     AKLIQLRRSHPNLHRRKFFQDRVIRGSVVRDIAWYNTDGNELSDEAWNTAWNRSLALM
                     LNGKTLAITDEEGNPIVDDSFLILVNAAAEGVEFKLPEPPAATQWTQVLNTENIEDPF
                     AAEELGEHVIVGGRALRVFQDGNK"
     gene            5684..7207
                     /gene="gltX"
                     /locus_tag="VHE33_16720"
     CDS             5684..7207
                     /gene="gltX"
                     /locus_tag="VHE33_16720"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HVT99151.1"
                     /translation="MPPSAQPPRVRFAPSPTGFLHVGNARTALYNWLFARHNGGVFLL
                     RIEDTDLDRSEARHEKQLMEDLLWLGIDWDEGPGVEGPHAPYRQSERLGIYREHTDRL
                     LAEGKAYRCFCTAAQLEEERRLAATEHQPQVYSGRCRTISDADSEARAGNGEPFAVRL
                     RIPDHPLRFHDIVRGVVEFPNETVSDPILVRSSGMPVYNYVVTLDDALMEITHVIRGD
                     DHISNTPKQVAIYEAFGWRPPEFAHLSTILGSDRERLSKRHGATSVASFREMGILPEA
                     LVNYLALLGWGAEGGTREIFSLDELVKEFRLERVTASPAIFDWEKLHWLNRHYLKAAD
                     SERVAELAWPHFAAAGFLAARDNASSDPRVDAWFGTLLALLLPSANQLDELPAKAAFV
                     FRFDPAAARANEENAAILATDSAQEVLAAFARRVAAHTGSVTAEQFKIWMNEIKAETG
                     AKGKDLFHPVRIALTGAHSGREFDKLIPVLEDGSQLDVPSHVLSVRERIEQFMSCGR"
     gene            7204..7362
                     /locus_tag="VHE33_16725"
     CDS             7204..7362
                     /locus_tag="VHE33_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99152.1"
                     /translation="MNTVLWIGEANPSRLAFVLRPRGGDGLQADLEEARGEGIDVLVS
                     MLTSEDNK"
     gene            7405..7692
                     /locus_tag="VHE33_16730"
     CDS             7405..7692
                     /locus_tag="VHE33_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99153.1"
                     /translation="MRFISHPILDRCTPSDLAGFRLLVRELRDQVRAGRSIGAHCRGC
                     IGRATVLLASVMIALGWKADEALRLIEQARGFQVPDTPEQLEWILRFQPEP"
     gene            7662..8255
                     /locus_tag="VHE33_16735"
     CDS             7662..8255
                     /locus_tag="VHE33_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99154.1"
                     /translation="MDPQVPARTVTLVFPHSWQAEILPKRPLIKPLRCYTYPREAEEV
                     ERGALEVMVQPAGRAAQFLATFALGFADSAAPTGLWACPDPDELCALAGGYAYIVDTA
                     DPAKFTHIGFRPVVEVGSLPEHKLLVFVGHQALLAWGAHGEAWQTRRLSSEGLRLTEI
                     CGNELHGFGWDLITDREVPFVVDLRTGGATQPATDSR"
     gene            complement(8231..8800)
                     /gene="rsmD"
                     /locus_tag="VHE33_16740"
     CDS             complement(8231..8800)
                     /gene="rsmD"
                     /locus_tag="VHE33_16740"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HVT99155.1"
                     /translation="MRVIAGQYRSRLLQAPRGLDTRPTSDRLRETLFNVLAPRIEGSV
                     FLDLYAGSGAVGIEALSRGAREVILVEQSEAANRVIRANLASLGIRGGYALETRSVSA
                     ALRRLAETGRQLDLAFLDPPYAEAEEYKRALGLLGGECSALLAEDALVIAEHEKHREL
                     EARYGTLVRYRVLKQGDAALSFYRESVAG"
     gene            8863..10008
                     /locus_tag="VHE33_16745"
     CDS             8863..10008
                     /locus_tag="VHE33_16745"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HVT99156.1"
                     /translation="MRAWSLPLGRWFGVHLRIHYFFLLLLIFCVLSTNLSGIASWRGV
                     FLWFLLFGAVLGREVARALTAAWHGLTVRSILLLPIGGLFSYSSPEMAERASEGSTQW
                     ALTLAGPLSNLGFAGLVTALIEGATSQIRIAALPIVTPAHLMRSTIWLNIALAVINCV
                     PAYPLDAGRLMRSSLSRTRGVAQATRSASGLGQMLGIGAVVAGLALLAIPNTHLASSL
                     SPWFIMGGFFVFIGAQLEDQGVLFQSVVETVRMRDVMLTDFDTLSPSDTLSDALYKAI
                     HSLQDDFPVVRGAALVGVVSRQGIIQALRHGGNGYIQAIMSRAFQVAQPDDSLGALIR
                     RIGGGRLSLIPVAESGRIVGIVTWQNLSSSMGLLAEYRRLERVDTES"
     gene            10005..10880
                     /gene="rsmI"
                     /locus_tag="VHE33_16750"
     CDS             10005..10880
                     /gene="rsmI"
                     /locus_tag="VHE33_16750"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HVT99157.1"
                     /translation="MNDEPLAPGLYIVATPIGNLEDITLRALRVLRSVDRIACEDTRQ
                     TRKLLDRYGITTPTISSHEHNEAERSRQLIEELRSDARIAVVSDAGTPGISDPGMVLA
                     TAAIASGIPVFSIPGPNAAVTALVASGLNTDRFSFVGFLPPKAGARRTALEDLARSTT
                     AATTLILYEAPHRILETMADLEAVWGSDCRIVIGRELTKLHEEFLRGTVREIREQLTQ
                     RESIRGEIVLLVDAQPAPVRTASGATTVQGRLAELEQSEGLAEKDALKRIARERGVSK
                     SDVWRELQRERARKK"
     gene            complement(10886..11374)
                     /locus_tag="VHE33_16755"
     CDS             complement(10886..11374)
                     /locus_tag="VHE33_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99158.1"
                     /translation="MPADRAEDSYAIYSLLMPGKTLASLASEQGASWAIAAVTISEGQ
                     RSPQVPPQGQLKPPPGNTKGFDEAVSDYEANKYSRVQLDKNDFHLDHPFALVNPDTAK
                     SAGYPGVTYFSEVYFDSRHRAALVYMYEWCADLCAAGSWVYLEKRGGRWQRQSGIVAP
                     GA"
     gene            complement(11641..14448)
                     /gene="acnA"
                     /locus_tag="VHE33_16760"
     CDS             complement(11641..14448)
                     /gene="acnA"
                     /locus_tag="VHE33_16760"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HVT99159.1"
                     /translation="MNSFGSQSELKSGGSKYEIFRLSALEKRGINLHRLPFSLRILLE
                     NLLRYEDGKSVTADDIEFLAKWNPKAEPSREISWMPARVLMQDFTGVPAVVDLAAMRD
                     AMKLLGGDPQKINPLQPAELVIDHSVQVDEFGTGNAYSVNAALEFQRNRERYAFLKWG
                     QTAFRNFSAVPPGMGICHQVNLEHLARVVFTTTGAKPQAYPDTLVGTDSHTTMINGLG
                     VLGWGVGGIEAEAAMLGQPVSMLVPQVVGFKLTGRLREGATATDLVLTVTEMLRKLGV
                     VGKFVEFYGPGIAELPLADRATIGNMAPEYGATCGLFPVDAETLRYLRLTGRNAEQIA
                     LVEAYFKEQGLFHTPDAPEAEYSQTIALDLAQVEPSVAGPKRPQDRVLLREAGASFKK
                     QLPSLLGPNADKVGQRQAIRWEGEGGHRSANGNVQSTHAAPEPGSNGNGHISSTAKER
                     FGVDVEPYLDHGSIVIAAITSCTNTSNPSVMVAAGLLAKKAVEKGLTVPPWVKTSLAP
                     GSRVVTDYYDKAGLTQWLDKLRFNTVGYGCTTCIGNSGPLPSDISKAIEDHSLVAVSV
                     LSGNRNFEGRINSEVRANYLMSPPLVVAYALAGRIDHDFDNDPIGNDTAGKPVFLRDI
                     WPSQQEVSTVIAAAITSESYTKEYATVTKGDANWQKLSFPTGDVYQWEQDSTYIRKAP
                     YFDGMPAEPAAVREISGARVLAVLGDSVTTDHISPAGSIKLNGPAGKYLTEHGVKPAD
                     FNSYGSRRGNHEVMVRGTFANVRLRNKLAPGTEGGVTRLLPEGEPMSIYDASVKYAER
                     GTPLVILAGKEYGSGSSRDWAAKGPRLLGVRAVIAESFERIHRSNLVGMGILPLQFEA
                     GANVDSLGLTGEETYDFLGLKALLDAKFANGKTIKVRATANGSTKEFNATVRIDTPQE
                     ILYFEHGGILQYVLRQLAAQK"
     gene            complement(14660..15271)
                     /locus_tag="VHE33_16765"
     CDS             complement(14660..15271)
                     /locus_tag="VHE33_16765"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HVT99160.1"
                     /translation="MLRVDPEGTAPEKIYKLMIGVIVPRPIAFVSSVDEHGIRNLAPF
                     SYFTGCSTNPPVVCFVTAVRSGPRPSKDTLHNVLATNEFVVNIVSEEFAGQMNATSAE
                     VGPEVDEFELAGLTPMASELVKPPRVAESHIHMECRLRQVLHISDRPGGGSLILGDVL
                     RFHIDQAIVDNYKVDPQKLRAIGRMGGPTYVRTGDRFDMPRPK"
     gene            15505..16935
                     /locus_tag="VHE33_16770"
     CDS             15505..16935
                     /locus_tag="VHE33_16770"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HVT99161.1"
                     /translation="MSAEAQPEHRVRVLAHEESAVHEEGQHSLEGVHGTVEVPHHQAG
                     FWRQWRAFVGPAILISVGYMDPGNWGTDLQAGAQFRYGLLWVVGLASLMAIFMQVIAA
                     RLGVVTGKDLAQCCRDWYPRWTRWPNWLMSEIAIAACDLAEVLGSAVALNLLFHIPLL
                     WAVIITGLDVLLLLALQRFGMRTIEAVVVLLVATIAACYFIELFVLPATHPSFVEMGR
                     ALVSPRFGQFGMIYVAIGIIGATVMPHNLYLHSALVQSRRLQKDEPSVRSAIRFNTID
                     SSIALSTAFFVNAAILVLAATVFFGKGSVTLPGGELVHFSADSDWIRIAYLTLAPLLG
                     TAAASTLFAVALLASGQSSTITGTLAGQVVMEGFMRWRIQPWVRRLITRTLAILPAVV
                     IIGIRGSSSVTDLLMLSQVVLALQLPFAMFPLLQFTSSRRRMGRWKNGWFLLMAGWAS
                     AVLITCMDLYGLPDSLSAAWRVIVGQ"
     gene            16932..17363
                     /locus_tag="VHE33_16775"
     CDS             16932..17363
                     /locus_tag="VHE33_16775"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVT99162.1"
                     /translation="MSEASMYGTILVTLEGTPTDRAIIEHVLELARLAGSRVVLLHVA
                     DGWAARTYGPDAVSPEIAEDTSYLAAVRAEFAAAGIPVESELAYGDPATEIVNWIERK
                     GCDLVAMSTHGHRFVKDMFLGTTVNQVRHSVDVPVLLLRAK"
     gene            17424..>18452
                     /gene="purD"
                     /locus_tag="VHE33_16780"
     CDS             17424..>18452
                     /gene="purD"
                     /locus_tag="VHE33_16780"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HVT99163.1"
                     /translation="MKVLVIGGGGREHALCWAIRRSSRVSEVVCAPGNGGIAQVARCV
                     KVDQKDIDDLLRVVASEKPDLTVVGPELPLSLGLVDAMQARGFRVFGPTLNAAMLETS
                     KAHAKRFLQRHNIPTANYAVCESRNEAQQALEMFKLPVVIKADGLAAGKGVLICETKE
                     QAAQAIADLFTGQLLGSAEKTVVVEEYLTGEELSFLVLSDGKHAVPLVPAQDHKRIGE
                     GDTGPNTGGMGVYSTDTMLDDAMRAWIVEHVAQKAIDGMAAEETPFVGVLYCGLMMTA
                     TGPQVLEFNARFGDPETQAILVRMESDLVEALEACVEGRLSDSEFRWKPGASACVVAA
                     SQGYPGSYE"
BASE COUNT         3363 a         5715 c         5642 g         3732 t
ORIGIN      
        1 gctgcctgca gtggaggcct tccgcattga atattggctc ctcgaagaag ccaagttgcg
       61 ccgccagccg gcggaaaaag aactcagccg ccgtattgct ctgattgtcg gaggcgcaag
      121 tggcattgga cgcgccgctg ctctcatcgc cgcggagcgc ggagcccacc tcatgattgc
      181 cgaccgcgat ctcgccggag ccgaagcggt cgcacaggag ctgcgtgcct ctgcgggcaa
      241 ggagactatc gccgccaccg ctgtcgatat tcgcgaccgt tcttcgatcg ccaaagcact
      301 gcgcgagacg attgcccgct tcggtggcct cgacatcctc atcaacaccg cggctatttt
      361 tccgtcttcg ccacagggcg ccatcagtga cgcgatgtgg tcgacaactc tcgacctcaa
      421 cgtgactgcg aactatctgc tggcggaaga agcagcaaga atttttaaca cacagaagat
      481 cgatggcagc atcgtcctta ccagctctgc gaacgcggtc gttcccaagc gcggcagcga
      541 agcttatgac gtgagcaaag ccgcgctgag ccacctcatc cgtgagctgg ccgtttcgct
      601 ggcacctcac gtccgcgtga acggcatcag ccccgcgacc gtggtgaaag gctcgacgat
      661 gtttccccgt gaccgagtca tcacgtcgct gaggaaatac gatatagggt tcgagccatc
      721 gatgacggat gaccagctcc gcgatctgct cgccggcttc tatgcccatc gcacgctgac
      781 tcatcagccc attgagccgg aagactgtgc gcgcgccatc ctcttccttg ccgggccgca
      841 cacgcgttgc acaaccggcc accttattcc tgtagacggc ggtctgcctg aggcgttcct
      901 ccggtgagct attttccggc gctcgtcgcg attgatcttg gcgcggagag ctgcagggtt
      961 tccctgctgc gctggcgggg gggcgaagcg agtatcacga tggcacgccg cttcgagaat
     1021 gggccgcagc atcatggcgc cgccggctgg cactggaacc tcgaccgcat ttgcaacgag
     1081 ctcgaagccg gtctgcggac ttgcgcggag tggtcaccgg agggcatcgc cgccatcggc
     1141 gttaccggtt gggccgtcga ctacgtccga ctcgatccgt ttggcaggcc gcttaccgca
     1201 ccgttttgtt accgcgacgc tcgcaatcag cgagcgatgg ccgaagtcca cgccgccgtt
     1261 cctgccgaaa agctctatgc gcgcaccggg gtgcagatcc aaccgatcaa cacgatctat
     1321 cagctctacg ccgaccgcag cagcggtatt cccgattcgg ctccgtgggt taatttgccg
     1381 gagtacatcc tgcactggct tggcgcgcca cgcgtcgctg agtacaccaa cgctactcac
     1441 acgggcctca tcgacggcga cacgcggcaa tggtccggtg agatcttcgc cgcattgagc
     1501 cttgatcgtg ctgcggcacc tgaaatcgtc gcacctggaa cgacgctcgg tccggtgcgc
     1561 gatgatctgc gccgcctgcc cgctttcgct cagacgcaac tcattgcacc ggcctgccac
     1621 gacacggcct cggccattgc cggcattccg gaacgacgcg gtgactgggc ctacatcagc
     1681 tcaggaacct ggtcccttgt cggtatgcca ctcacgcaaa gcctgcgtac gcaagaggcc
     1741 ttttcgcgtg gatttaccaa cctcggcgct gctgatggcg gaactctttt ccaccgcggc
     1801 ctgccgggca tgtggctgct gcgccagtgc atgaacacct gggatcagga acgcggatgg
     1861 caattcggcg acctcatcgc cgaggcgagc cgaatgccgg cgccggatcg tctgctggat
     1921 ctggacgacc ccgcgctgct cccgccgggc gacatgaccg cgcgcatcaa tgagcagcgt
     1981 cgtcgtctcg gttcaccttc gctgccctcc ggctgtgacg cagcgcctca ctatgcgagc
     2041 ctcatctttc gcagcctggc cgcgcgctac ggaacattga tcgctgaaat tgcagaaatg
     2101 accggagaaa agccgcatca catcgccgtt gtcggcggtg gcagccagaa tgaatacctc
     2161 aacgcgctca ccgccgaggc tacaggactt ccagtgcagc ggtgttcggc tgaaagctcc
     2221 acactgggaa atttcgccat acaatgggcg cggatcgagc agtcctcagg cacgctccag
     2281 ccccaatcgg ttgccgaaaa ggtctccgcg ctcgccgccg ttcaggtcgc ttagcgccga
     2341 aaggaacccg catgtccact gccattcttg gcggaattgg atggcatatg gtcggtgcgg
     2401 ctgcagccgc ctccttctat gcgcccattg agaaggtgaa gaagtggtcc tgggaaacca
     2461 cgtgggcggt ggcgggaatg ttctcctgga tcctgctgcc catcattgtc agcctcgtcc
     2521 tgctgccgga tttccatgcc ttctacgctt cgatcccgag cgacgtgctt ttgaagacct
     2581 tcctatttgg ctgcatgtgg ggggtgggca atgtgaatta cggcctcacg atgcgctacc
     2641 tcgggatgtc gctaggcatc ggcgtcgcca tcggcgtcac gctggtcgtg gggacgctgg
     2701 tcccgccgat cgtgcacggg caactcggct atctgattac cagccggagc ggccacttca
     2761 ccctttccgg cattgtgctg gcgcttattg gcatcgcgac tgtttcctgg gccggccatc
     2821 aaaaggaaca gcgcatgggc atccgcaccg aggaattcaa tctgacgaag gggctgttgc
     2881 tcgccgtcat gtgcggcatc ttctcgtcgg gcatgtcttt cgccatcgac gccgcgcggc
     2941 ccatcgcctc cgccgcacag ttggccggcg tcaaaccgct ctacgccgcg cttcccagct
     3001 acgtcatcat catggggggc ggcgcgatcg tcaatttcgc ttactgcttc tcgcgtcttg
     3061 cctttcgcag ggacctgtcg cttcgctccg atctatcgca aacgaagcgg accctcgttc
     3121 tgaacagttc tcttgccgca acggccggca tcatgtggta tttgcagttt ttcttctacg
     3181 cgtggggtgc tgccaatatt cccgttcatc tcagcttcgt caactggatg ctccacatga
     3241 gcatgtatgt tctcttcggg ggtattgtcg gcctggctct gggtgaatgg cgtggtgcac
     3301 ggggccgtcc cgtacagttg ctttggtccg gaatggtcat catcatcgca gcggccaacc
     3361 tggttgggct gggaatggca acctgacccg tggggttttc tcattgtttt taggtcgcaa
     3421 acgcggtggc gatttctgca tctgactatt cgctgaacca ttccctcgtg agagtcgtgc
     3481 atgcatcgaa cgctgcttcc tggcaagccc tatcctctgg gcgccacccc aacgagtatc
     3541 ggaaccaact tcgccctctt ctctgaaaac gcaaccggcg tctctgtttg cttcttcgac
     3601 gaaaacaaca accagatcga ttgcgttgct ctgcgcgagc gaactgcctt cacctggcac
     3661 ggtcttgtcc gcggcatcaa gcccggtcag cgttacggct atcgtgtgga aggcccgtgg
     3721 gagccggaga aagggctgcg cttcaactcc aataaactgc tcgtcgatcc ttacgcgaag
     3781 gcgattagtg gggaggtgga ctggaaggcg ccaatattcc cttacgacgt gaccaccggg
     3841 aacgatctca cccgcgacga tcgtgattcg gctgccggcg tccctaagtc cgtcgtcatg
     3901 gatccgcact ttgactggga gggcgactgt ccgcccgaga ttccgctcgc cgactccatc
     3961 atttacgaag tgcacgtccg cggtttctcc aaactcaacg aagctgtgcc ggagaacctt
     4021 cgcggcactt acgccggact cgcctgcgag ccgagcatcg aatatctgaa aaagctgggc
     4081 atcactgcgg tcgaactact ccctgtccat catttcatcg acgagggcca tctcgtggat
     4141 cggggtttgc gcgactactg gggctacaac acgctgggct acttcgcacc catgtcgcgc
     4201 tacagttctt ccggcgatat ggggcaacag gtcgctgagt tccgccagat ggtcaaggcg
     4261 ctgcacaagg cggggatcga ggtcatcctc gacgtggtct ataaccacac ctgcgaaggg
     4321 aatcgcatgg ggccgatcct ttccatgaag gggatcgaca atacgacgta ctatcgccag
     4381 gtcaggcacg atccacgcta ttacatggat tacaccggca ccggaaacac gctcaacgta
     4441 atgaatcccc aggtgctgaa gctggtgatg gactccctgc gctactgggt gacggagatg
     4501 cacgtcgacg gtttccgctt cgaccttgcc agcacgctgg cgcgcgagct gcatgccgtg
     4561 tcgaagttgt cgagcttctt cgacacgatt caccaggacc ccaccatcgc cgatgtaaag
     4621 ctaatcgccg aaccctggga cgtaggcgag ggtggctatc aggtcggtaa ctttccggtg
     4681 ctctgggccg aatggaacgg aagatatcgc gatacggtgc gccggttctg gaagggcgac
     4741 agcgggctgc tctctgactt tgcttaccgt ctcaccgggt cctcagacct gtatgagatg
     4801 gatggccggc gccccaacgc ctctatcaac tttgtcacgg ctcatgacgg cttcacgctt
     4861 tgcgatttag tcagctataa ccacaaacac aacgaggcca atggcgacaa caatcgcgac
     4921 gggaccgacg acaaccactc ctggaacatg ggtgcggaag ggccgaccga cgatccggct
     4981 atcaatacac tgcgcgagcg acagatgcgc aactttctcg caacgctgat tctctcgcag
     5041 ggggtgccca tgctctgcgg cggcgatgag ctggcgcgct cgcagaaagg caacaataac
     5101 gtttactgcc aggacaatga actcgcatgg ttcaactgga aactgactcc ggccgaggac
     5161 aggctgcgcg acttttcggc taagctgatt cagctgcgcc gcagccatcc aaacctccac
     5221 cggcggaagt tcttccagga tcgcgtgatt cgcggatcgg tggtgcgcga catcgcctgg
     5281 tacaacactg acggtaacga actgtccgac gaggcctgga acactgcctg gaaccgctcg
     5341 ctggcgctga tgctcaacgg caaaacgctg gccatcaccg acgaggaagg caatcccatc
     5401 gtcgacgaca gttttcttat cctggtgaac gcggccgccg aaggtgtgga attcaagctg
     5461 ccggaaccgc cagcagcgac gcagtggacg caggtgctga ataccgagaa catcgaggac
     5521 ccgttcgcgg cagaagaact cggcgagcat gtaattgtgg gaggaagggc tctgcgtgtc
     5581 tttcaggacg gcaacaagtg acccgcggat gcgacaacac gggaggctcg ggcaggttgc
     5641 cgcatcgtcc tcgcttcctt aagatcaaag attgatcccg ctcatgcctc cgtccgccca
     5701 gccccctcgc gttcgctttg cgccttcgcc tactggattc cttcacgtcg gcaatgcacg
     5761 caccgcgctc tacaactggc tcttcgcccg gcacaacggc ggcgtcttcc tgttgcgcat
     5821 tgaagatacg gaccttgacc gcagcgaggc gcgtcacgaa aagcagctga tggaagacct
     5881 gctgtggctc ggcatcgatt gggacgaagg tccgggtgtc gaaggtccgc acgcacctta
     5941 tcgtcaatcg gaacggcttg gcatttatcg cgagcatacg gatcgtctgc ttgccgaagg
     6001 caaagcctat cgctgcttct gcactgccgc ccagctcgaa gaagagcgcc gcctagcggc
     6061 cacagagcac cagccacagg tttattccgg ccgctgccgg acaatcagcg acgcggactc
     6121 cgaggcacgt gcgggcaacg gtgagccttt cgcggtgcgt ctgcgcatcc ctgatcaccc
     6181 gcttcgcttt cacgatattg tccggggggt cgtcgaattc cccaatgaaa cggtgagtga
     6241 tcccattctg gttcggtcca gcggcatgcc ggtttacaac tacgttgtca cgctcgacga
     6301 cgcattgatg gagatcacgc atgtgattcg cggtgacgat cacatctcga acacgcccaa
     6361 gcaggtcgcg atctacgagg cctttggatg gaggccaccg gagtttgcgc atctctccac
     6421 gatccttggc agcgatcggg aacggctctc gaaacggcac ggtgcaacgt cggtcgccag
     6481 ttttcgtgaa atgggcattc ttcctgaggc gctcgttaat tatctggcat tgctcggatg
     6541 gggcgccgaa gggggaacac gcgaaatttt ctcgctggac gaactagtga aggaatttcg
     6601 tctggagcgc gtcaccgcat cgccggccat ctttgactgg gagaagctgc actggctcaa
     6661 tcgccactat ttaaaggcgg ccgattccga gcgcgtcgcg gaactcgcct ggccgcactt
     6721 tgcggcagca ggcttcctgg cagcgcgtga caacgcatcg tccgatcccc gggtcgacgc
     6781 gtggttcggc acactgctgg cgctgttgtt accttcagcg aatcagctcg acgaattgcc
     6841 agcgaaagcg gcgttcgtct ttcggtttga tcccgcggct gcgcgggcaa acgaagagaa
     6901 cgcagccatt ctcgcaaccg actccgcgca ggaggttctt gccgccttcg cgcgacgggt
     6961 cgctgcgcac actggttccg tcaccgcgga gcagttcaaa atatggatga acgagatcaa
     7021 agctgaaacc ggcgccaaag ggaaggatct gttccatcct gtgcgcatcg ccctcaccgg
     7081 ggcccattcc ggccgggaat tcgacaaatt gattccggtg ctggaagacg gcagccaact
     7141 ggacgtgcca agtcacgttc tcagcgtgcg tgagcgcatc gaacaattca tgagctgtgg
     7201 ccgatgaata cggttctctg gatcggggaa gcgaatcctt cccgccttgc attcgtgctg
     7261 cgtccaaggg gcggcgatgg cctgcaggcc gatcttgaag aggcgcgcgg cgaaggcatc
     7321 gacgtgcttg tctcgatgct cacttctgag gacaataaat aactcggcct cacggaagag
     7381 ggccgcatcg cggaacaact tggcatgcgc ttcatctctc acccgattct cgaccggtgc
     7441 acgccctccg acctagccgg ctttcggctc ctggtgaggg aacttcgaga tcaggttcgc
     7501 gccggtcgca gcatcggcgc gcattgccgc gggtgcattg gccgcgccac ggtcctgctc
     7561 gcctcggtca tgatcgcact gggttggaaa gcggatgagg ctctgaggct gatcgagcag
     7621 gcacgcggct tccaggtacc cgatacgccg gagcagttgg aatggatcct caggttccag
     7681 cccgaaccgt gacgctcgtc tttccgcact catggcaggc agaaattctg ccgaagcggc
     7741 ccctcatcaa gccgctgcgc tgctacacgt atccgcgcga ggcggaagag gtagaacgcg
     7801 gcgctctgga ggtgatggtg cagcctgctg ggagggcggc ccaattcctc gcgacttttg
     7861 ctctcgggtt tgccgattct gccgcaccta ccggtctttg ggcctgtcct gatcccgatg
     7921 aactctgcgc gctggcaggt gggtacgcct acatcgtcga cacggctgat ccggcgaagt
     7981 tcacccacat cggcttccgg cccgtggttg aagtgggttc tttgccggag cacaagctgc
     8041 tcgtttttgt cggccatcag gcattgctgg cctggggagc ccacggggaa gcctggcaga
     8101 ctcgacggct ctcctcggaa ggcctccggc tgaccgaaat ctgcggcaat gaactgcacg
     8161 gctttggctg ggatctcatc acggatcgcg aggttccctt tgttgtcgat ctgcgaaccg
     8221 gcggtgccac tcagcctgcc acggattcgc ggtagaagct caaggccgcg tcgccctgct
     8281 tcagcacacg atagcggacg agcgtaccgt agcgagcctc caactcgcgg tgtttctcgt
     8341 gttccgcaat caccagggca tcctctgcca ggagcgccga gcattcgccg ccgagtagcc
     8401 ccagagcccg cttgtactcc tccgcctccg cgtagggcgg atcgagaaat gccaggtcca
     8461 gctggcggcc cgtctccgcc agtctccgca atgcggcgct cacgctgcgc gtctccagcg
     8521 catagcctcc acgaatccca aggctcgcca ggtttgcacg aatcacgcgg ttcgcggctt
     8581 ctgactgttc caccaagatg acctcccgcg cccctcggct cagcgcctca atgcccaccg
     8641 cgcccgagcc ggcatacaga tcgaggaaga ccgatccctc gatccgcggc gcgagcacgt
     8701 tgaacagtgt ttcgcgcagc cggtcgctgg tgggccgggt gtccagcccg cgcggagcct
     8761 gcagaagccg cgaccgatac tgtcctgcaa tcacacgcat gcctactccc ggaaaaatcc
     8821 atcgaccctc tacaataacg ggaaggacct taggggaacg agatgcgcgc gtggtcacta
     8881 cctctcggac gctggtttgg tgtgcacctg cgcatccact atttctttct tcttctcctg
     8941 attttctgcg tcctttccac caacctctca ggcatcgcgt cgtggcgcgg cgtcttcctc
     9001 tggttcctgc tcttcggcgc tgtgctggga cgcgaagtcg ctcgggctct tacggctgcc
     9061 tggcacggtc tcacggtgcg cagcattctg ctgctcccta ttggcgggtt gttctcctat
     9121 tcgagtccgg agatggccga gcgcgcctcc gagggttcga cgcagtgggc gcttaccctt
     9181 gctggtcccc tctcgaatct ggggttcgcg ggactcgtta ccgccctgat cgaaggagct
     9241 acctcgcaaa tccgcatcgc cgcgctgccg attgttacgc cggcacatct gatgcgctcc
     9301 acgatctggc tcaacatcgc cctcgccgtc attaactgcg tccctgctta cccgctcgat
     9361 gcaggccgcc tgatgcgcag tagtttgtcg cggacccgcg gtgtcgcgca ggccacgcgc
     9421 tccgcctcgg gcctcggcca gatgcttggc atcggcgctg tcgttgccgg cctcgcgctg
     9481 ctggccattc cgaacacgca tctcgcgtca tctctcagtc cctggttcat catgggtggg
     9541 ttctttgtct tcatcggcgc ccaactggaa gaccagggag tgctcttcca gtccgtcgtc
     9601 gaaacggtac ggatgcgcga cgtcatgctg acggactttg acacgctctc gccttcggac
     9661 accttgtccg acgctctgta caaggctatc cacagcctgc aggacgattt cccggtggtt
     9721 cggggagcag cgctggtcgg cgtcgtttcg cgtcagggaa tcatccaggc gctgcgccac
     9781 ggcggcaacg gctacatcca ggccatcatg tcgcgtgcct ttcaggtcgc tcaacccgac
     9841 gactcgctcg gcgcgctcat ccgccgcatc ggcggcggac gcctctctct gatccccgtt
     9901 gctgaaagcg gccgcatcgt cggcatcgtc acctggcaaa acctcagcag cagtatgggg
     9961 ctgctggccg agtaccgccg actcgagcgc gtcgacaccg aatcgtgaac gacgaacccc
    10021 tcgcgcccgg actgtacatc gtcgccacac ccatcggcaa tcttgaggac atcaccctgc
    10081 gagcactgcg cgtgctgcgc tcggtggacc gcattgcctg cgaggacacg cggcagacgc
    10141 gcaagctcct cgaccgctac ggcattacca cgcccactat cagcagtcat gagcacaacg
    10201 aggccgaacg gagccgtcag ctcatcgagg agttaagaag cgacgcgcgc attgccgttg
    10261 tttccgatgc agggacgccg ggcatttccg atccaggaat ggtgctcgcg accgcagcca
    10321 tcgcttcagg cattcccgtg ttttcaattc ctggcccgaa cgccgcggtc acagccctgg
    10381 tcgcctcagg gctgaacacc gatcgtttct ccttcgtagg atttctaccg ccaaaagcag
    10441 gcgcccgcag gacagcactc gaggacctgg ctcgttcgac gacagccgcc actacgctga
    10501 tcctgtatga ggcgccgcac cgcatcctgg aaaccatggc cgacctcgaa gctgtctggg
    10561 gctcggattg ccgcatcgtg atcggccgcg aactcaccaa gctgcatgaa gaattcctgc
    10621 gcggcacggt tcgcgagatc cgcgagcagc tgacccaaag agagagcatc cgcggagaga
    10681 tcgtgctgct tgtcgatgcg cagcccgcac cggtgcgaac cgcgagcggc gcaacgaccg
    10741 ttcaggggcg ccttgccgaa ctggagcaat ccgaaggtct ggcagagaaa gacgccctca
    10801 agagaatcgc gcgcgaacga ggcgtttcga aaagcgatgt ctggcgcgag ctgcagcgcg
    10861 aacgcgcgcg gaagaaatag aaggcctacg cgccgggcgc cacgatgcct gactgccgct
    10921 gccatcggcc gccgcgcttt tcgagataga cccagctgcc ggccgcgcac aggtcggcgc
    10981 accactcgta catgtacacc agtgccgccc ggtgcctgct gtcgaaatag acttcgctga
    11041 aatacgtcac gcccggatag ccggcactct tcgccgtgtc gggattcacc agcgcgaaag
    11101 gatggtccag gtgaaagtcg tttttatcca gctgcacacg cgagtactta tttgcttcgt
    11161 aatcgcttac ggcttcatcg aagcccttcg tattgccagg aggcggtttt agctgtccct
    11221 ggggaggaac ttgcggactg cgctggccct cactgattgt taccgctgca attgcccacg
    11281 aagccccctg ctccgatgcc aggctcgcca gcgtcttgcc cggcattagc agcgagtaga
    11341 tcgcgtaaga gtcctccgcc cgatctgctg gcatcgaagt aatgccgcct ggcgcggacg
    11401 aggctgtggt caatgttgcg ggttgctgtg aaccgctgct ctgccccaac actgtcagac
    11461 agcacgacag cagcccgatc gcaacctgac tgtgtctcac cgcgcgcaat cgcatgtctg
    11521 catagacgcg gcagaatgtg gtggcgttcc tgcatttttc aaatttttcg agctctatcc
    11581 ccgtataccg cgccacaaaa gagggccgct cagggccggc cctcggattt ggagacgggt
    11641 ttacttttgc gctgccagct gtcgcaacac atattgcaga atgccgccgt gctcgaagta
    11701 gaggatctcc tgcggggtgt cgatgcgcac cgttgcattg aactctttcg tcgagccgtt
    11761 cgcagtggcg cgtaccttga ttgtcttacc gttcgcgaat ttcgcgtcga gcagggcctt
    11821 gaggccgagg aagtcgtagg tctcctcgcc ggtcagcccc agcgaatcga cgttcgcgcc
    11881 cgcttcgaat tgcagcggga ggatgcccat tcccaccaga ttcgatcggt gaatgcgctc
    11941 gaagctctcc gcgatcaccg cacgcacgcc caggagccgc ggtcctttcg ccgcccagtc
    12001 gcgcgaagag cccgagccat attccttacc tgccaggatg acaagcggtg tgcctcgttc
    12061 ggcatacttc accgacgcgt cgtagatact catcggctcg ccttcgggca gcaaccgagt
    12121 gacgccgcct tcggtgcccg gcgccagttt gttgcgaaga cgcacgttgg caaaggtccc
    12181 acgcaccatc acttcgtgat tgccgcgccg cgagccatag gaattgaaat ccgccggttt
    12241 cacgccgtgc tccgtcagat atttcccggc cgggccgttc agcttgatcg atcccgccgg
    12301 cgatatatgg tcggttgtca cggaatcgcc caaaacggcc aatacccgtg cgccggagat
    12361 ctcccgtacg gcagcaggtt ccgccggcat cccgtcgaag tacggagcct tgcggatgta
    12421 ggtcgagtcc tgctcccact ggtaaacatc gcccgtaggg aagctgagct tctgccagtt
    12481 ggcgtcgccc ttcgtgacgg tcgcatactc cttcgtgtag ctttccgacg tgatggccgc
    12541 cgcgattacc gtcgagacct cctgttgcga aggccagata tcccgcagga aaaccggttt
    12601 cccggccgta tcgttcccga ttggatcgtt gtcgaagtcg tggtcaatcc gccctgcgag
    12661 cgcgtaggca acgaccagag gcggcgacat caaatagttg gcgcggactt cggaattgat
    12721 gcggccctcg aagttgcgat tgccgctcag caccgaaacc gccacgaggc tgtgatcctc
    12781 gatggccttc gagatgtctg agggcagcgg gccggagttg ccgatgcacg ttgtgcagcc
    12841 gtagccgacg gtgttgaatc gcaatttgtc gagccattgc gtcaggccgg ccttgtcgta
    12901 atagtccgtc accacgcgcg acccgggcgc gagtgatgtc ttgacccatg gcggcacggt
    12961 cagtcccttt tcgaccgcct tcttcgccag cagtccggcc gctaccatca ccgacggatt
    13021 cgatgtattc gtgcagctcg taattgcagc gatcacgatc gaaccgtggt cgagataggg
    13081 ttcgacatcc acaccaaagc gctccttcgc ggttgatgag atgtgtccat tgccattcga
    13141 acccggctcg ggggcggcgt gggttgattg gacattgcca ttcgccgacc tatgcccgcc
    13201 ttcgccctcc cagcgaatcg cctgccgctg accgaccttg tctgcgttgg gtccaagcag
    13261 cgacggtagc tgttttttga aactggctcc agcttcgcgc aggagaaccc tatcctgagg
    13321 acgcttcggg ccggcgacgc tgggctcaac ctgggcaagg tccagtgcga tggtctgcga
    13381 gtactctgct tccggagcat cgggcgtatg gaagaggccc tgctctttga aatatgcctc
    13441 gacgagcgcg atctgttcgg cattgcgacc ggtcaggcgc aaatagcgca gcgtctccgc
    13501 gtcgaccggg aaaagaccgc aggtcgcgcc atactcgggc gccatgttgc cgatggtcgc
    13561 gcgatccgcc agcggcagtt cggcgatccc agggccgtag aactcgacga atttgccgac
    13621 aacgccgagc ttgcgcagca tctcggtcac cgtgagcaca aggtcggtag ccgtggcgcc
    13681 ttcgcgcagt ctgccggtca gtttgaaccc gacgacctgc ggcaccagca tcgagactgg
    13741 ctggcccagc atggctgcct cggcttcgat gccaccgacg ccccatccca ggacgcccag
    13801 gccattgatc atcgtggtat gcgagtcagt cccgaccagc gtgtcgggat acgcctgcgg
    13861 tttcgcgccg gtggtcgtga acacgacccg tgccagatgt tcgagattga cctggtggca
    13921 gatgcccatg cccggcggga ccgccgagaa gttccgaaac gccgtctgtc cccacttgag
    13981 gaaggcgtag cgctcgcggt tgcgctggaa ttcgagcgcc gcgttcaccg agtacgcatt
    14041 gccggttccg aactcatcga cctggacgga gtggtcaatc accaactcgg cgggctggag
    14101 gggattgatt ttctgcggat cgccgccaag cagtttcatc gcatcgcgca tcgcggccag
    14161 atcgaccacc gccgggacgc cggtgaagtc ctgcatcagg acgcgagcgg gcatccagga
    14221 gatctcgcgg gaaggctcgg ccttcgggtt ccacttcgcg aggaactcga tgtcgtccgc
    14281 ggtgacggac tttccatcct cgtagcggag caggttttcc agcaggatgc gcaggctaaa
    14341 gggcaggcga tggaggttga tgccgcgttt ctcgagcgcg ctgaggcgga agatctcata
    14401 cttgctgccg ccggacttga gttcggactg gctgccaaag ctgttcatga cgtttccttt
    14461 ccagttggag ggcgcaaggg gctcgctcac gcgctgaacg ggcggagcgg gaggaacagt
    14521 cagttcgaag agatacgtcg tcctcgatca ggacggccgc ggcgaaagcg ccggagcaag
    14581 ggggacgaga tcgctcggtg cacgatcatc gcctcccatt ttagagccaa tcgcgcctgt
    14641 ccgaacagac ctcgtcggct tacttcggcc ggggcatgtc gaagcgatcg ccggtgcgga
    14701 cgtaggtggg accgcccatc cgcccgatcg ctctcagctt ctgcgggtcc accttgtagt
    14761 tatcgacgat tgcctggtcg atgtgaaagc gcagtacatc gccgaggatc aggctgccgc
    14821 cgccgggccg gtcgctgatg tgcagaacct gccttaaccg gcattccatg tggatgtgac
    14881 tttcggcgac gcgtggcggc tttaccaact cgctggccat gggggtgagg cccgccagct
    14941 caaattcatc gacctcgggg ccgacctctg ccgaggttgc gttcatttgt ccggcgaact
    15001 cctccgagac gatgttcacc acgaattcat ttgtagccag cacgttgtgc agcgtgtctt
    15061 tggagggtcg cgggccggag cgcactgccg tcacaaagca gacgactggc gggttggtgc
    15121 tgcagccggt gaaataactg aacggggcca gattccgtat gccgtgctca tcgaccgagg
    15181 acacaaaggc gatcggccgc ggcacgatca cgccgatcat caacttgtag attttctccg
    15241 gagccgtgcc ctccgggtca acacgcagca tttccatggc ttctgcggga ttggatgcgc
    15301 gtgggcagcg ctcgattcgc ggcgggcgag gaggaggaac tttgttatga acatttctta
    15361 acacttaggt gttaaccatg gtttacaagc gcagcccaat cgacgtacac tcctgaaaac
    15421 ggcgcggctc cagacgctca cccagaggct ggaatgtcac tgcgcggact attggaacaa
    15481 gcaggaactg cgaatcgagc tgggatgagt gcagaagcac aacccgagca cagggtgcgc
    15541 gtattggccc atgaagagag cgctgtccac gaagagggac aacactcatt agaaggggtg
    15601 catggcaccg ttgaggtacc gcaccaccag gctggattct ggagacagtg gcgagccttc
    15661 gttgggccgg cgattctcat cagcgttggt tacatggatc cgggcaactg gggcaccgac
    15721 ctgcaggcag gtgctcagtt ccgctacgga ctgctgtggg tcgtaggtct tgccagtctc
    15781 atggctatct tcatgcaggt gattgccgcg cgccttggcg tggttaccgg caaggatctg
    15841 gcccagtgtt gccgcgactg gtatccgcgg tggacgcgat ggcccaactg gctcatgagc
    15901 gaaatcgcta ttgccgcctg cgatctggca gaggtgctgg gcagcgccgt cgccctcaac
    15961 ctgctctttc atattcccct cctctgggcg gtcatcatta ccgggctcga tgtgttgttg
    16021 cttttggctc tgcagcgctt cggtatgcga accatcgaag ccgtggtcgt gcttctggtg
    16081 gccacgattg cggcgtgtta ctttatcgag ctgttcgtcc ttcctgcgac ccatcccagc
    16141 tttgtggaga tgggaagggc tctggtttcg cctcgcttcg gccagttcgg gatgatctat
    16201 gtcgcgatcg gaatcattgg cgcgacggtc atgccgcaca acctttattt gcattcggcc
    16261 ctggtgcaga gccgcaggct gcagaaggat gagccgtcgg tccgcagtgc gatccgcttc
    16321 aataccatcg actcgagcat cgcgctgagc acagcgttct tcgtgaacgc ggcgattctg
    16381 gttctggcgg cgacagtgtt ctttggcaaa ggcagcgtca ctctcccggg cggtgagctg
    16441 gtgcatttca gcgcggacag cgactggatt cggattgctt acctgaccct tgcaccgctg
    16501 ctgggcactg cggccgcaag cacgctgttt gctgtggcgc tgctggcgag cggccagagc
    16561 agcaccatta caggaacgct cgccggccag gtggtgatgg aaggctttat gcgctggcgc
    16621 attcagccat gggtacggcg cctgataacg cgtacgctgg ccattctgcc ggcggttgtc
    16681 atcatcggca ttcgaggcag cagcagcgtc accgatcttt tgatgctgag ccaggtcgtg
    16741 ctcgcgttgc agcttccctt cgccatgttt cctctgctgc agttcaccag ttcccgcagg
    16801 cgcatgggaa gatggaagaa cggctggttc ctgctgatgg cgggctgggc gagcgcggtt
    16861 ctgattacct gcatggatct ctacggcctg ccggactccc tgagcgccgc atggcgggtc
    16921 attgtgggcc aatgagcgaa gcgagcatgt acggcacgat tctggtgaca ctggaaggca
    16981 ccccgacgga ccgtgccatc atcgagcacg ttctggagct ggccaggctc gccgggagcc
    17041 gcgtggtgct gctgcatgtc gccgacggct gggcggcccg aacgtacgga ccggatgctg
    17101 tcagcccgga gatcgccgaa gacacctcgt acctcgcggc ggtccgcgca gagtttgcgg
    17161 ctgccggtat cccggtcgaa tcggagctgg cctacggcga tcccgcaacc gagatcgtga
    17221 actggatcga gcgaaaggga tgcgacctgg tggcgatgag cacccatggt caccgcttcg
    17281 tcaaagacat gttcctgggc accacggtca accaggtgcg gcacagcgtg gatgttccgg
    17341 ttctgctgct ccgggcaaag tgacgctggg cgctggatgc actgctgacg gccgctgcgg
    17401 ctgctgatag gctcggatac aagatgaagg tgctggtcat tggtggagga gggcgcgagc
    17461 acgcgctctg ctgggcgatt cgacgctcgt cgcgcgtgag cgaagtcgtg tgtgcgccgg
    17521 ggaacggcgg catcgcgcag gtggcgcgtt gcgtgaaggt ggatcagaag gacatcgacg
    17581 atctgctgcg cgtcgttgca tccgagaagc ccgatctgac tgtcgttggg ccggagctgc
    17641 cgctttcgct gggtctggtg gatgcgatgc aggctcgcgg gttcagggtc ttcgggccca
    17701 cgctgaacgc ggcgatgctg gagacgagca aggcgcacgc caagcgcttc ctgcagcggc
    17761 acaacattcc gacagccaac tacgcggtct gcgaatcccg caatgaagcg cagcaggcgc
    17821 tggagatgtt caagctgccg gtggtgatca aggccgacgg gctggctgcc ggcaagggtg
    17881 tactgatctg cgagacgaag gaacaagccg cgcaggctat tgccgatttg tttaccggac
    17941 agctgctcgg atcagccgag aagacggttg tcgtcgagga gtatctgacc ggcgaggagc
    18001 tgtcgtttct ggtgctgagc gatggaaagc atgcggttcc actggttccg gcgcaggacc
    18061 acaagcggat cggcgaaggc gatacgggac cgaatacggg gggcatgggt gtttactcaa
    18121 ctgacaccat gctcgacgat gcgatgcggg cctggatcgt cgagcacgtc gctcagaagg
    18181 cgatcgatgg gatggcggcg gaagagacgc cgttcgttgg ggtgctctac tgcgggctga
    18241 tgatgacggc gaccgggccg caggtgctgg agttcaacgc gcgcttcggc gatccggaga
    18301 cgcaggcgat cctggtgcgg atggagagcg atctggtgga ggcgctcgag gcttgcgtcg
    18361 aaggccggct cagcgacagc gagttccgct ggaagccggg cgcgtcggcg tgcgtggttg
    18421 cggcttcgca gggctatccc ggcagctacg ag
//
LOCUS       DASZYN010000649         4156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_864126, whole genome shotgun sequence.
ACCESSION   DASZYN010000649 DASZYN010000000
VERSION     DASZYN010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864126"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VHE33_16785"
     CDS             complement(<1..476)
                     /locus_tag="VHE33_16785"
                     /inference="COORDINATES: protein motif:HMM:NF014714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99164.1"
                     /translation="MATPKIFISAGEASGEYYGTLVLEAIRHLQPNATFFGLGGRRME
                     ALGFRPIVRSEDVAVMGITEVVRHLPRIYREYRRLRASISAENPDIAILIDFPDVNLS
                     LARRLKAQNVPVIYFVSPQLWAWKKYRIRSVQRYVDRMLTIFPFEERFYHDHGVDA"
     gene            complement(478..2538)
                     /locus_tag="VHE33_16790"
     CDS             complement(478..2538)
                     /locus_tag="VHE33_16790"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT99165.1"
                     /translation="MPGSEPQSRKNPQGATPEPRKTAKPSSAQQDEDVVGKAYDSRLM
                     GRLIRYLYPYKAACFTSIVAILLKAGADVLGPYLTMVAVDLYLKPAADRTPQMAAADA
                     AMGAAHHPFLAHWLTPWASGGAWTGVSQIAGIWLAALVLSYGLEALQTWLMQWTGQKV
                     MFDMRSQIFRHLQGMHIGFFDRNPVGRLVTRLTTDVDAINEMFTSGVFAIFEDIFVLA
                     AIVFVMLRMKWWLALLAFSVLPLILLVTRIFRKYVRESYRRIRAAIARINAYTQEYIS
                     GMTLVQLFNRQQRAFSDFSTINRIHMEAYKDNILCYALYYPAVELLSSVAIALIIWEG
                     GGGVLRGTVMLGTLVGFMQYAQRFFRPIQDLSEKYNILQAAMAASERIFKLLDTSPEI
                     VSPENPKPGDGSGRIEFRDVWFTYQRLDREAKDRIATASSADLQAMADIEWVMRGVSF
                     TIEPNQTAAIVGHTGAGKTTITSLMMRFYDVQRGRVLVDGVDVREQDLTALRQRFGVV
                     LQDPFLFSGTVADNIRLGTAHITDDDMQIAAEQVNVDDFIQSLPGAYEEPLRERGAGL
                     STGQKQLINFARALAHRPGILILDEATSSVDTDTEFRVREALGRMVEGRTSVVIAHRL
                     STVQRADVILVMHRGQLREMGSHQELLAMHGIYWKLYQLQYKDQELAEPSTGLTPSLA
                     LGTD"
     gene            2628..3128
                     /locus_tag="VHE33_16795"
     CDS             2628..3128
                     /locus_tag="VHE33_16795"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT99166.1"
                     /translation="MSLIRPCRADEGAAILRIINAAAAAYRDAIPADRWHEPYMRMEE
                     LEQEIAAGVEFQGYEADGALTGVMGIQAVQDVDLIRHAYVLPGSQGRGIGGALLRHLR
                     DLSSRPMLVGTWAAAEWAIRFYQRHGFEMVSPDEKNRLLRKYWAIPERQIETSVVLAN
                     PALVKN"
     gene            3281..3697
                     /locus_tag="VHE33_16800"
     CDS             3281..3697
                     /locus_tag="VHE33_16800"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT99167.1"
                     /translation="MTYTLDSSLDSVNKVEQTAEQLARKAGVDEDEIFRISMAVREAA
                     VNAVLHGNAYDPEKRMTASFENTGTSLVIRITDEGKGLDPSTLPDPLAPENLLRGSGR
                     GIFLIRSFMDEVHFKMLHPGTELTLIKHLGTAPTGA"
     gene            3744..4088
                     /locus_tag="VHE33_16805"
     CDS             3744..4088
                     /locus_tag="VHE33_16805"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVT99168.1"
                     /translation="MKISTRQVDGVTILDLGGRITLGEGSVQLRDSVRDLLAKGQKKI
                     LLNLGDVNYIDSSGIGELVSAYTTVRNQGGELKLLNLTRKVHDLLQITKLYTVFDVKD
                     DEASAIASYARA"
BASE COUNT          805 a         1140 c         1430 g          781 t
ORIGIN      
        1 tcggcgtcga cgccgtggtc gtggtagaag cgctcttcaa agggaaagat ggtgagcatg
       61 cggtcgacgt aacgctggac gctgcggatg cggtatttct tccaggccca aagttgcggg
      121 ctgacgaagt agatcacggg cacgttttgc gctttgaggc ggcgagcgag ggagaggttg
      181 acgtcgggaa agtcgatgag gatggcgatg tcggggttct cggcggagat ggaggcgcgc
      241 aggcggcggt actcgcgata gatgcggggc aggtggcgca cgacctcggt gatacccatg
      301 acggcgacgt cttcggagcg gacgatgggg cggaagccga gggcttccat gcggcggccg
      361 ccaaggccga agaaggtggc gttgggctgg aggtggcgga tggcttcgag gacgagggtg
      421 ccgtagtatt cgccgctggc ttctccggcg gagatgaaga ttttgggggt ggccaagcta
      481 gtccgttccc agggccaggg aaggggtcag ccctgtgctt ggctcggcga gttcctggtc
      541 cttgtactgg agctggtaga gcttccagta gattccgtgc atggcgagga gttcctggtg
      601 ggaacccatc tcgcggagct ggcctcggtg catgacgagg atcacgtcgg cgcgctgtac
      661 tgtcgagagg cgatgcgcga tcacgaccga ggtgcggcct tcgaccatgc ggccgagggc
      721 ttcccggacg cggaattcgg tgtcggtgtc gaccgaggag gtggcttcgt cgaggatgag
      781 gatgcctggc cgatgggcca gcgccctggc aaaattgatg agttgctttt ggccggtgga
      841 gagaccggct ccgcgctccc gcagcggctc ctcgtaggct ccggggagcg actggatgaa
      901 gtcgtcgacg ttcacctgct cggcggcaat ttgcatgtcg tcgtcggtga tgtgcgccgt
      961 gccgaggcga atgttgtcgg cgacggtgcc gctgaagagg aaggggtcct gaaggacgac
     1021 gccgaagcgc tggcgcaggg cggtcagatc ctgctcccgg acatccacgc catcgaccag
     1081 cacccggccg cgctgcacgt cgtagaagcg catcatgagg ctggtgatcg tggtcttccc
     1141 tgccccggta tggccaacga tagccgcggt ctggttcggt tcgatggtga aggaaacacc
     1201 gcgcatcacc cactcgatgt cggccatggc ctgaagatcg gcggaactcg cggtcgcgat
     1261 gcgatctttg gcctcgcggt cgaggcgctg ataggtgaac cagacgtcgc ggaactcgat
     1321 gcggccggag ccgtcgccgg gtttcggatt ttcaggggag acgatctccg gcgatgtgtc
     1381 gagcagcttg aagatgcgtt cgctggcggc catcgcagcc tgcaggatgt tgtacttttc
     1441 gctgaggtcc tggatgggcc ggaagaagcg ctgggcgtat tgcatgaagc cgaccagggt
     1501 tcccagcatg acggtgccgc gcaggacgcc gccgccgcct tcccagatga tgagcgcaat
     1561 ggcgaccgag ctgagcagct cgacggccgg gtagtagagg gcgtagcaga ggatgttgtc
     1621 cttgtacgcc tccatgtgaa tgcgattgat ggtggagaag tcgctgaagg cgcgttgctg
     1681 gcggttgaac agctgcacca gcgtcatccc cgaaatgtat tcctgggtat aggcgttgat
     1741 gcgggcgatg gcagcccgaa tccgccggta cgattcgcga acgtacttgc ggaagatgcg
     1801 ggtgaccagc aggatcagcg gaagaaccga aaaggcgaga agggccagcc accacttcat
     1861 gcgcagcatg acgaatacga tcgccgcgag tacgaagatg tcttcgaaaa tcgcgaagac
     1921 accggaggtg aacatttcgt tgatagcgtc gacgtcggtg gtgaggcggg tgacgaggcg
     1981 gccgaccggg ttgcggtcaa agaagccgat atgcatgccc tgcaggtggc ggaagatctg
     2041 gctgcgcata tcgaacatga ctttttgtcc ggtccactgc atcagccagg tctgcagagc
     2101 ttcgagtccg tagctgagca ccagggcagc cagccagatt ccggcgattt gcgaaacgcc
     2161 cgtccatgct ccgccggatg cccacggggt gagccaatgc gccagaaacg ggtgatgagc
     2221 agcacccatc gcggcgtcag ctgccgccat ctgcggagtc ctgtcagcag ctggtttcag
     2281 gtagaggtcg acggcgacca tcgtcagata gggcccgagc acatcggcac cggccttgag
     2341 caggatcgcg acgatggagg tgaagcacgc ggctttatag ggatagaggt atcggatcag
     2401 ccggcccatg agccggctgt cgtaggcttt gccgacgacg tcttcatcct gctgggcaga
     2461 actgggtttt gccgtcttcc tgggctcagg agtggctccc tgcgggttct ttcgagactg
     2521 gggctcagag ccgggcatgg gtggctcttt ttatcttacc ggggagggca gaagggagac
     2581 gacccggaac ccgaactcga cctggcacgc caatgcgaat atgggctatg agcctgattc
     2641 gaccctgccg cgccgatgag ggagcggcca ttctgaggat catcaatgca gccgccgcgg
     2701 cctaccgcga tgcgattccc gccgaccgct ggcacgagcc ttatatgcgg atggaagagc
     2761 tggagcagga aatcgcagct ggcgtggaat tccaggggta tgaagccgac ggcgcattga
     2821 ccggcgttat ggggatccag gcagtccagg atgtcgatct gattcggcac gcctatgttc
     2881 tgcccggctc gcagggacga ggcatcggcg gtgcgcttct ccgccatctg cgcgacctca
     2941 gctcccgccc gatgctggtg ggaacctggg cggcggcgga atgggccatc cgcttttacc
     3001 agcggcacgg gttcgagatg gtctcgcccg atgagaaaaa ccgcttgctg cggaaatact
     3061 gggccattcc cgagcgccag attgagacgt cggtcgtgct ggccaacccg gctctcgtca
     3121 agaattgagt caacagcagc gaccccgctg gaggcatccg atcttccctg cttgacatct
     3181 tccgctccag caagcaaact tcgcctacgg gaaaaagtcc ccccaagacc cgcgatcacc
     3241 aggcgaggaa gggcatttga caggacagcc ggaaaagcgc gtgacctaca ctctggattc
     3301 ctcgctggac agcgtcaata aagtcgagca gaccgccgag cagttggctc gcaaggcggg
     3361 tgtggatgag gatgagatct tccgcatctc gatggccgtg cgggaagccg cggtgaacgc
     3421 agtattgcac ggcaacgcct acgatccgga aaagcggatg acggcgtcgt ttgaaaacac
     3481 cgggacttca ctggtgattc gaatcacgga tgagggcaag ggtctcgatc catcgacgct
     3541 gccggatccg ctggcgccgg agaacctgct gcgcggttcc ggacgcggga tcttcctgat
     3601 tcggtctttt atggatgagg tacacttcaa gatgctgcac ccgggaaccg agctgacact
     3661 catcaagcat cttgggaccg ctccgaccgg cgcctgactt ccccgcacat tcaagttttt
     3721 ccaaggagga atttctcgtg agcatgaaaa tcagcacccg tcaggtggac ggggtaacga
     3781 ttctcgatct cggcggacgg atcaccctcg gcgagggcag tgtgcagctg cgtgattcgg
     3841 tccgtgacct gctcgccaag ggccagaaga agattctgct gaacctgggc gatgtgaatt
     3901 acatcgacag ctcagggatc ggcgagctgg tcagcgcgta tacaaccgtg cgcaatcagg
     3961 gcggcgaact gaagctgctg aatctgacgc gcaaagtgca tgacctgctg cagatcacca
     4021 agctgtatac cgtgttcgac gtgaaggacg acgaggcgag cgccatcgcg tcctacgctc
     4081 gcgcctgaat ttttgccggc ttctgtgaaa aggggctgcc cgagggcagc ccttcgtgtt
     4141 tgaggacgat cggcgc
//
LOCUS       DASZYN010000650         5013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1062046, whole genome shotgun sequence.
ACCESSION   DASZYN010000650 DASZYN010000000
VERSION     DASZYN010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062046"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VHE33_16810"
     CDS             <1..654
                     /locus_tag="VHE33_16810"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT99169.1"
                     /translation="ELAAAVRPRWTALVRDRNVWGIVLGRALTDPIWWFYVFWLPQYL
                     SDARGFDLRHIALFAWIPFVAADLGNFTGGLVAGYLIRRGYGVLPVRKWLCAACCLPI
                     LVGILVPRVSNSWLALGLICIALWGYAAWSTMGLTLPGDLFPHTIVGSVTGLSGLAAG
                     LAGTAFTLVVGATVDRFSYAPAFLIAGVMPIAATLSVFALIRPPVAAEPVLLQNFDR"
     gene            821..2362
                     /locus_tag="VHE33_16815"
     CDS             821..2362
                     /locus_tag="VHE33_16815"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HVT99170.1"
                     /translation="MRRYRFLILAVACCAGITAQAHADSSCPDLASLKIEGVEITAAT
                     EVPANAPLPASYPHYDGSLPAHCRVEGMMHRRKGVDGVEYGIGFAVALPEKNAWNGDY
                     MMQGGGGSNGILYYPAGNSYSGDQSALQRGFAVGSTDTGHQSRTGFDFSFMRDQQAFL
                     DFADQANAEAAGVAKRIISAYYAKPAAYSYFVGCSTGGREGMILSQRFPTVFNGIVVG
                     DPAMRTGLSNLAIGQWIPVAYNQAAPKDSSGKPEIDKLFTDADRRLFMDALMKKCDAL
                     DGVKDGMIFDPLGCHFDPEEIACKSGQTESCMAPEKVAAIKKAFAGPKNAYGTQVYPG
                     FLYDAGIAASGPLPGLLSPRSRGLFGPVTTATTLDVDKAALHASDPLVEPASTNLTTF
                     SGNGGKLIFFHGDSDPWFSPLDTFQYYESLAPANGGADKVAAWSRFFFVPGMSHCGGG
                     PSLDRFDMLGAVVNWVEKGTAPESVIAVGKAFPGRSRPLCAYPKHAQYSGHGNTEDAA
                     SFVCQ"
     gene            2438..4447
                     /locus_tag="VHE33_16820"
     CDS             2438..4447
                     /locus_tag="VHE33_16820"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HVT99171.1"
                     /translation="MTPSRIIPAAAAALAVLLFAGPIAFAQNAPPPFPVPPPLNAPLP
                     FVSPIFGDNMVLQRGKPDAIWGWSKPGDAVRIQIGNRTATATAGADRRWEAKIDPPSA
                     GGPYTLTVSGPQTVEFHNVLVGDVWLCGGQSNMELPLRFTANAEEVAKTADDPEIRYF
                     TVAGRPAYHTANLIGGSWRVVSPQTADWLSAVAFYFARRVQQDTHIPIGLVVDAVGGT
                     PAEAWTSAAALRTMGADFDVPLAELQRLAGSGAPEYGNYILHWYDEYDTGLKEKWFAP
                     GLDDASWKPVPVPNGFSELGLPDTPAVVWFRREITLPDPLPAVRATIHLGEIERMDTV
                     YINGTEVGASAWVENPRVYSLRPGVLRPGKNLIAIRVFKTKPDGGFLGKPDELYLALD
                     DQKIPLAGDWKAKLSVDARPPHPMPIGFENWPVIPTVLYNGMLEPIVPLSITGALWYQ
                     GEQNSPRGYQYRRLLPVMIADWRSTFAQGDFPFYIVSLPAFGPRSSTPTDDAWADTRE
                     SQAIAAAAVPNSCLAVTIDTGDPDSLHPKNKQPVGDRLAFCALANNYHRHVVDMGPTI
                     KSVHRKSGSIVLRFSHTNGGLVVRGDKLGEFSIAGDDRKWYWASATIKGNTVIVSSPS
                     VPHPTQVRYAWQSNPEATLFNGAGLPAAPFRTDDWPLVTQTARPY"
     gene            complement(4501..>5013)
                     /locus_tag="VHE33_16825"
     CDS             complement(4501..>5013)
                     /locus_tag="VHE33_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99172.1"
                     /translation="TDFLKMGAYTMAVSTLSYNDSAATYNSRGLLYKMYRDHFGTIPV
                     DVSGNSPQPAPKYPPYGDQPKTSSGSPTYPLDMVAALTADRKYLTIAVVNGTDSYQKF
                     DLTVAGMRATGPATRWELTGKDLDSENRVGDSPQVSVRETSMDEKPGSISVPAISVDI
                     YRFPVTPTGQ"
BASE COUNT          895 a         1723 c         1388 g         1007 t
ORIGIN      
        1 gaactcgccg ccgccgtgcg tccccgctgg acggctctcg tgcgcgatcg caacgtctgg
       61 ggcatcgttc tcggtcgtgc gctcaccgat cccatctggt ggttctacgt cttctggctc
      121 ccgcaatatc tcagcgacgc ccgcggcttc gacctgcggc acatcgcgct ctttgcctgg
      181 ataccgttcg tcgccgccga tctcggaaac ttcaccggcg gcctcgtcgc aggctacctg
      241 atccgccgcg gatacggtgt ccttcccgtg cggaagtggc tctgcgctgc gtgttgcctg
      301 cccattctgg tcggcattct cgttccccgc gtatcgaatt cctggctggc cctgggcctc
      361 atctgcatcg cgctctgggg ctatgcggcc tggtcgacca tggggctcac gctgcccgga
      421 gatctctttc cccataccat cgtcggttcc gtcaccggtc tcagcggtct cgccgcagga
      481 ctggcgggca cggctttcac gttggtagtt ggggccaccg tcgaccggtt ctcctacgca
      541 ccggcgttcc tgattgccgg tgtcatgccg atcgctgcga ccctctccgt gtttgctttg
      601 atccggccgc cggtcgccgc agagccggtt ctcctgcaga acttcgaccg atagacgatc
      661 tggattgtgg ttacagccga ggttagactg ttcagcagat cccttcgcgg ccgcgcgtgc
      721 gcggcccaca gaacctggcg gtccacgctg cataaaacga aaactgtttc gtggccggcc
      781 cccgcccgca gatcgaatct cggagaaaga aggccacacc atgaggcggt acagatttct
      841 gattctggct gtcgcctgct gtgcaggcat cacggcccag gctcacgccg actcctcctg
      901 cccggacctc gccagtctca aaatcgaggg cgtcgagatc acagccgcta ccgaagtccc
      961 ggcaaatgcg ccgcttccgg cctcatatcc gcattacgac ggttcgctgc ccgcgcattg
     1021 tcgcgttgag ggcatgatgc accggcgcaa gggggtcgac ggtgtcgagt acggtatcgg
     1081 ctttgctgtt gctttgcccg agaagaacgc ctggaacggc gactacatga tgcagggcgg
     1141 aggcggcagt aacgggatcc tctactatcc cgccggcaac tcatattccg gcgatcaatc
     1201 ggctctgcag cgaggattcg cggttggcag caccgatacc ggtcaccagt cgcggaccgg
     1261 ctttgatttt tccttcatgc gcgatcagca ggcatttctg gacttcgctg atcaggccaa
     1321 cgccgaagcc gctggcgttg cgaagcggat catttctgcc tactatgcca agcccgccgc
     1381 ttattcctac ttcgtcggct gctccaccgg cggccgcgaa ggcatgatcc tctcgcagcg
     1441 tttccccaca gtctttaacg gcattgtggt cggcgatccc gccatgcgca ccggcctttc
     1501 gaatctggcc atcggccagt ggattccagt ggcgtataac caggccgctc caaaggattc
     1561 gtcgggaaag ccggagatcg acaagctctt taccgacgcg gatcgccggc tcttcatgga
     1621 cgcgctgatg aagaagtgtg acgcactcga cggagtgaag gacggaatga tcttcgatcc
     1681 cctcggctgt catttcgatc cggaagagat tgcctgcaaa tcgggtcaaa ccgaaagctg
     1741 catggctccg gaaaaagttg ccgccataaa gaaggccttc gcgggaccga agaatgctta
     1801 cggcacccag gtctatcccg gctttctcta cgacgcggga attgccgcct ccggtccgct
     1861 gccgggcctg ctgagcccgc gctcccgcgg tctcttcggc cccgtcacaa ccgcaaccac
     1921 cctcgacgtg gacaaggctg ccctgcacgc ctccgatccc ctcgttgagc cggcctcaac
     1981 caacctcacc accttctccg gcaatggcgg caagctcatc ttcttccacg gcgacagcga
     2041 tccctggttc tcccccctcg atacgttcca gtattacgag tcgctcgctc cagccaatgg
     2101 gggagccgac aaagtagccg cgtggagccg gttcttcttc gtccctggga tgagccactg
     2161 cggcggcggt ccctcgctcg atcgcttcga tatgctgggc gccgtcgtga actgggtcga
     2221 aaaaggaacc gcgccagagt ccgtcattgc tgtcggaaag gcgtttcctg gacgcagccg
     2281 cccgctctgc gcatatccca aacacgcgca gtacagtgga catgggaaca ccgaggacgc
     2341 tgccagcttc gtgtgccagt aatgtgattc gttccaggct ctccggaacg aaaccaaagt
     2401 cctcgtccgc cagcaatctg cccgcaggag aactgcattg acgccatccc gaatcattcc
     2461 tgccgccgcc gcggccctcg ctgttctgct cttcgctggc cccattgcct ttgcgcagaa
     2521 tgctccgcct ccctttccgg taccgccccc gctgaatgcg ccattgccgt ttgtcagtcc
     2581 catcttcggc gacaacatgg tgctccagcg tggcaaaccc gatgcgatct ggggctggtc
     2641 caaacccggt gacgccgttc gcatccagat tggcaatcgc accgccaccg ccaccgccgg
     2701 agcggatcgc cgctgggagg cgaaaatcga ccctccttct gcgggcggtc cctataccct
     2761 caccgtttcc ggtcctcaaa ccgtcgagtt ccacaacgtg ctcgtgggcg acgtctggct
     2821 ctgtggcggc cagtccaaca tggagctgcc tctgcgcttc actgcaaacg cggaggaggt
     2881 cgcaaaaacc gccgacgatc ccgagattcg ctacttcacc gtcgcgggcc gtcccgctta
     2941 ccacaccgcc aatctgatcg gcggatcgtg gcgcgtggtt tcgccgcaga cagccgactg
     3001 gctttccgcc gtcgctttct acttcgcgcg cagggtacag caggacaccc acatccccat
     3061 cggccttgtc gttgatgccg tcggtggaac tccggctgaa gcctggacca gcgctgcagc
     3121 gcttcgcacc atgggcgccg acttcgatgt gcccctcgcc gaactccagc gtctcgccgg
     3181 ctccggcgcg cccgagtacg gcaactacat cctccactgg tacgacgagt acgacactgg
     3241 ccttaaagag aaatggttcg cacccggcct tgacgatgcg agttggaaac cagtccccgt
     3301 tcccaatggt ttctcagagc tgggcctccc cgacacaccc gctgtcgtgt ggttccgcag
     3361 agagatcacc ctgcccgatc cgcttcccgc cgtccgcgcc accatccacc tcggcgagat
     3421 cgagcgcatg gacaccgtct acatcaacgg aaccgaggtc ggggccagcg cctgggtcga
     3481 gaatccgcgt gtctattccc tgcgtcccgg cgtgctcagg ccaggaaaga atctcatcgc
     3541 catccgcgtc ttcaaaacca aaccggacgg tggcttcctc ggcaaacccg atgagctgta
     3601 ccttgccctc gacgatcaga aaatcccgct tgccggcgac tggaaagcca aactcagcgt
     3661 cgatgctcgg ccgcctcatc ccatgcccat cggctttgag aactggcccg tcatccccac
     3721 cgtcctttac aacggaatgc tcgagcccat cgtgcccctg tccatcaccg gcgccctctg
     3781 gtaccagggc gagcagaatt ccccccgcgg ctatcaatac cgcaggctgc ttcccgtcat
     3841 gatcgccgac tggcgcagta ccttcgctca gggcgatttc cccttctata ttgtcagctt
     3901 gcccgccttc ggtccccgca gctccacacc caccgacgac gcatgggccg acacgcgcga
     3961 gtcgcaggcc atcgctgccg ccgccgttcc caattcctgc cttgccgtca ccatcgacac
     4021 cggcgatccg gacagcctcc atccgaagaa caagcagccc gtcggcgatc ggctcgcctt
     4081 ctgtgccctg gcgaataact atcaccggca tgtggtcgac atgggcccaa ccatcaaatc
     4141 ggtccaccga aaatccggtt ccatcgtgct gcgcttctca cataccaacg gcggactggt
     4201 cgtcagggga gacaagctcg gcgagttctc catcgccggc gacgaccgca aatggtactg
     4261 ggccagcgcc acgatcaaag gcaacaccgt gatcgtctcg tcgccctctg tgccgcatcc
     4321 cacgcaggtt cgctacgcct ggcaatccaa tcccgaggcc accctcttca atggcgctgg
     4381 cctcccggcg gcgccgttcc gcacagacga ctggcccctc gttacgcaaa ccgcccggcc
     4441 atattgagca agcccgatag aaaccgcaat gtcaatgcaa aacgccacag caggtggcgg
     4501 ctattgccct gttggtgtaa ccgggaatcg atagatatcc acgctgatcg cggggactga
     4561 gatggagcca ggtttctcat ccatggatgt ctcccgcacg cttacctgag gcgaatcgcc
     4621 gacgcggttc tccgagtcga ggtctttacc ggtcaattcc catcgagtcg ccggccctgt
     4681 cgcacgcatg ccggccaccg ttagatcgaa tttctggtac gagtctgtgc cgttcacaac
     4741 agcaatcgtc aggtatttgc gatctgctgt gagagccgca accatgtcca gaggataagt
     4801 cggacttccg gaactcgtct tcggctgatc gccgtacggc ggatacttcg gcgcgggctg
     4861 gggcgaattg cccgatacat caaccgggat ggttccgaag tggtcgcgat acatcttata
     4921 gagcaagccg cgcgagttat atgtcgctgc cgaatcgtta tagctcagcg tggaaactgc
     4981 catggtatag gcgcccatct tcaggaaatc ggt
//
LOCUS       DASZYN010000651         3018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_736239, whole genome shotgun sequence.
ACCESSION   DASZYN010000651 DASZYN010000000
VERSION     DASZYN010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736239"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VHE33_16830"
     CDS             <1..621
                     /locus_tag="VHE33_16830"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HVT99173.1"
                     /translation="GQTSVQVAARFQKDVVELHPDSVHILVGTNDLKANWVPQTTWDA
                     FTSMIQEARAANIAVFIGTVPPWGDGPGARTSDPDYTGRNARVATLNGWLKAQAGVTV
                     IDYDSLLVGSNGLYNPAMTDDGIHPNAAGYAVMTPAALKALEWVSDQCSDTFARPEGR
                     PVELQGLRLSSFSTLSFGPFHAKFTSVQRTVAMGIASLHCHGGINA"
     gene            614..2329
                     /locus_tag="VHE33_16835"
     CDS             614..2329
                     /locus_tag="VHE33_16835"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HVT99174.1"
                     /translation="MRKTLSAVVSATAILVSLNATAQDRGRDRDWDRSPIRHVLLVSI
                     DGMHAVDFLNCSHGMAGINNGAPYCPNLAALGTTGVNYVNASTSKPSDSFPGLMTLVS
                     GATPRTLGVYYDVAYDRSLDGPMTATGNGNPAGNCTAGAAPTGYETEYEEGIDIDQTK
                     LNGGAPNAGLTDGGIASIDATKLDRDPAKGCAPVMPWQFVRTNTIFGVVHNAGGYVAW
                     SDKHPAYSSVAGQHGMLDDYYSPEINSNVVPLPGVTTASGTTCAAVPDPGSDLTAWTN
                     SFQNIQCYDQLKVNAILHEIDGMDHLGMTKTRTPTVFGMNFQAVSVGQKLVEGSTKGG
                     YLDGAGTPSASLLSEIQFVDDAIGQWVDELKDKGKYESTLIVITSKHGQSPIDPNLFF
                     PIPGKTNNGQSPATLLASLLPANQVNGVGATEDDVSLLWLADPSTLSSAVATLESNLK
                     TAGIGQIFYGPSLALNYDVPGLPPNGDPRTPDIIVTPNVGVTYTGSNKKLEEHGGFSH
                     DDTNVMLLVENPALRAQTVYTPVGTNQVAPSILLALGLDPRALDGVRLEGTGVLPYVN
                     LNFSW"
     gene            complement(2402..>3018)
                     /locus_tag="VHE33_16840"
     CDS             complement(2402..>3018)
                     /locus_tag="VHE33_16840"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HVT99175.1"
                     /translation="AIYHSQSGENFFTHTPGIRVVFPSNALDANGLLRTAIRCDDPVL
                     FLEHKRLYREAYGRAPYPGPDYMIPFGKANIVREGSHLTLITYGALVPRALQAAQRAQ
                     RELGIETEILDLRTLNPYDWEAIATSVTKTSKVIVAHEDTLSWGYGAEIAARIGEELF
                     DALDAPVRRIGALDSFVGYQPVLEDAILPQPEKIFHAIAELAKY"
BASE COUNT          600 a          812 c         1040 g          566 t
ORIGIN      
        1 ggacagactt cagtgcaggt ggcggcccga ttccagaagg acgtggtcga gctgcatccg
       61 gactcggttc acatcctggt ggggaccaat gacctcaagg cgaactgggt tcctcagacg
      121 acgtgggatg cgttcacgtc gatgattcag gaggccaggg cggcgaatat tgcggttttc
      181 atcggtacgg tgccgccgtg gggggatggg ccgggcgcgc gaaccagcga tcctgactac
      241 accggccgga acgctcgtgt tgcgacgctg aacggctggc tcaaagcgca ggcaggggtc
      301 acggtgatcg actatgactc gctcctcgtg ggtagtaatg ggctctacaa tccggcgatg
      361 acggatgacg ggattcatcc gaacgcagcg ggatatgcgg tgatgacgcc ggcggcgctg
      421 aaagcgctgg aatgggtcag tgatcagtgt tcagatacct tcgcaaggcc ggagggtcgc
      481 cctgtcgaat tgcaaggctt aaggttatct tcattttcca ccctatcttt cggcccgttt
      541 cacgcgaaat tcacgagcgt gcagcgaaca gtcgcaatgg gcattgctag tctccactgc
      601 catggaggca tcaatgcgta aaacgttgtc tgctgttgtt tctgcaactg caattctggt
      661 ttcgctgaac gcaaccgcac aggacagagg cagagatcgc gattgggatc ggagcccgat
      721 ccgccacgtt ttgctggtca gcatcgacgg catgcacgcg gtagatttcc tgaactgcag
      781 ccatgggatg gccggcatca acaacggcgc accctactgt cccaatctgg cggccttggg
      841 cacgaccggg gtgaactacg tcaacgccag tacctcgaag ccttcggatt cctttcctgg
      901 gctgatgacg ctggtgagcg gcgcgacacc acggacgctg ggggtgtact acgacgtcgc
      961 ttacgaccgg tcgctcgatg gaccgatgac agcaacgggc aatggaaacc cggccgggaa
     1021 ctgcacagcg ggcgcggcgc cgactggtta tgaaaccgag tacgaggaag gcatcgacat
     1081 cgatcagaca aagctgaacg gcggagcccc gaatgccggc ctgaccgatg gcgggattgc
     1141 ctcgatcgat gcgacgaagc tggatcgcga tccggcgaag ggatgcgcgc cggtgatgcc
     1201 gtggcagttt gtgcggacga atacgatctt cggcgtggtg cacaacgcag gcggctatgt
     1261 ggcgtggtcg gacaagcatc ctgcgtattc gtcggtggcg ggacagcacg gaatgctgga
     1321 tgactactac tcgcccgaga ttaactccaa cgtggtgccc ctgccgggag tgactacggc
     1381 ctcggggacg acctgcgcgg ccgtgccgga tcccggctcc gatttgacgg cgtggacgaa
     1441 cagcttccag aacattcagt gctacgacca gctgaaggtc aacgcgattc tgcatgagat
     1501 cgacgggatg gatcacctgg ggatgacgaa gacacggaca ccgacggtgt ttggaatgaa
     1561 tttccaggcc gtgagcgtgg ggcaaaagct cgtcgaaggc tcgacgaagg gcggctatct
     1621 ggatggagcc gggacgccgt cggcttcgct gctgagcgag atccagtttg tggatgacgc
     1681 gatcgggcag tgggtggacg agctgaagga caagggcaag tacgaatcga cgctgatcgt
     1741 cattacgtcg aagcacggtc aatcgccgat cgatccgaat ctcttcttcc cgattcctgg
     1801 caagacgaac aacggccagt caccggcgac actgctggcg agtcttttgc cggcgaacca
     1861 ggtgaacgga gtcggggcaa cggaggacga cgtctccctg ttgtggcttg ccgatccttc
     1921 gacgttatcg tcggctgtgg cgacgctgga aagcaacctg aagacggcag ggataggcca
     1981 gattttctat gggccgagcc tggcgctgaa ctacgatgtg ccgggactgc caccaaacgg
     2041 cgatccgcgc acgccggaca tcatcgtgac gccgaacgtg ggcgtgacgt atacgggcag
     2101 caacaagaag ctggaagagc acggcggctt ctcgcatgac gacacgaacg tgatgctgct
     2161 ggtcgagaat ccggcattgc gggcgcagac ggtgtacacg ccggtgggaa ccaaccaggt
     2221 ggcgccatcg attctgctgg cgctggggct ggatccgcgg gcgctggacg gcgtgcgact
     2281 ggaaggcacg ggtgttttgc cgtacgtgaa tctgaacttc agctggtgaa aaggacgtgg
     2341 gggcaggttc gcaggtacag ggcgcgcgtg ggtttcggtg ctcccgcgcg ctctctgtaa
     2401 gtcagtactt cgccagctcc gcgatggcgt ggaagatctt ttccggctgc ggaaggatcg
     2461 cgtcttcgag cacgggttgg tagccgacga acgaatccag cgcgccgatg cggcggaccg
     2521 gggcgtcaag ggcgtcaaag agttcttccc cgatacgggc ggcaatttcg gcgccgtagc
     2581 cccagctgag cgtgtcctcg tgggcgacga tgaccttgct ggttttggtg acggaggtgg
     2641 cgatggcttc ccagtcgtag gggttcagcg tgcgcaggtc gagaatctcg gtttcgatgc
     2701 cgagttcgcg ctgagcccgc tgggcagcct gaagagcacg cggcacgaga gctccgtagg
     2761 tgatgagcgt cagatggctg ccttcgcgaa cgatattcgc cttgccgaag gggatcatgt
     2821 agtcggggcc cgggtagggc gcgcggccat aggcctcgcg atagaggcgc ttgtgctcaa
     2881 ggaacagcac cgggtcgtcg cagcggatag cggtgcgcag caggccattg gcatcaaggg
     2941 cgttcgatgg gaagacgacg cggatgccgg gcgtgtgggt aaagaagttt tcgccggact
     3001 gcgagtgata gatggctc
//
LOCUS       DASZYN010000652         7723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_291602, whole genome shotgun sequence.
ACCESSION   DASZYN010000652 DASZYN010000000
VERSION     DASZYN010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7723
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291602"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VHE33_16845"
     CDS             <1..778
                     /locus_tag="VHE33_16845"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HVT99176.1"
                     /translation="DPTIDVMPVIPLDLTGQPDPNAGKSIDDLIAQAEKNRPEVAIYQ
                     MQAEVQRQALKDINSELLPRLDMYGVFAGNGTAGPANPFCNLGPQYCSTTLPTGFGGE
                     FQNLFNYTAPEYQVGMTLQINLRNRTFKADQFRAVLQYRQSQIASEQQQKSIRFDVRN
                     SQFALEQAQARVEAAQKSRDLAQHTFDITRQEQQLGAKSSADTLLTANALAVAESALD
                     AAQSAYEKAKVDIDRAIGDTLNRMQVSIDDAKSGVVTHGP"
     gene            818..2218
                     /locus_tag="VHE33_16850"
     CDS             818..2218
                     /locus_tag="VHE33_16850"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HVT99177.1"
                     /translation="MSTAAQPVIPEVSGSKLAPLVLIADDQPHILDALELLLEPEGYR
                     IEKATSPYMVLDALKSSSFDALLLDLNYTRDTTSGREGLDLLSQIRAHESQMPVIVMT
                     AWANVELAVEAMRRGASDFVQKPWDNARLLTILRTQIDLSNAVRRAQRLEAENRLLRA
                     ANLPEMIATAAVMQPVLATITRIGPSDASVLITGEHGTGKEVVAQTLHALSQRASRSM
                     VAVNTGALPEGTFESELFGHVKGAFTDARTERIGRFELAEGGTLFLDEIANVPLRQQA
                     KLLRVLETGEMERVGSSKTRHVNVRVISATNADIRKEVDAGNFRADLLFRLNTVEIHL
                     PPLRERREDIPPLAAHFLARYSQRYRKPVSGIDPTAMELMLHYAWPGNVRELDHTIER
                     AVLMSREEVIEVPDLGLSPARVSTQALEEMSLESVESILIRKALSRCNGNVSQAAEAL
                     GLSRGALYRRMEKYGI"
     gene            2304..3872
                     /locus_tag="VHE33_16855"
     CDS             2304..3872
                     /locus_tag="VHE33_16855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT99178.1"
                     /translation="MANHRGTSRRKPKRQSVSFEARLRIFCTVAALVFMALCAAVLTL
                     LHVTAGPMLATLGILGLILLLIFAAFIEEAVRPVQTLANVVAALREEDYSFRARGANR
                     DDSVGQLALEINALADTLQTQRVASLEAVALLRRVIAEMDAPVLAFDQETRLRLLNPA
                     AERVFSLEPAKHLGKTAEDLALTELLQETSETVLQLEIQRPSPLGVKRTEPSQPARWM
                     IRRGEFRQRGVPHTLVVLSDVSAALREEERLAWRRLIRVIGHEISNSLTPIKSIAGSL
                     RTRIVPKSANGSEPQAEPAPAPPPPIPPAMAVLQRDLQRGLSVIENRAESLNRFVQAY
                     RQLAQLPSPVLRRVPLQPLLERVVSLEIRLDVRLLSAPDVELLADTDQIEQLLINLVR
                     NAVESALSCVEEEPEPAPAIEASAENAARFAVRPRSQPTGTPCRPEVTIRATEESDLV
                     CIAIEDNGPGLANPENLFVPLYTTKKSGSGVGLALARQIVEAHGGIIELRNKPNHAGC
                     IAEVRIPIAEIPRT"
     gene            4282..5415
                     /locus_tag="VHE33_16860"
     CDS             4282..5415
                     /locus_tag="VHE33_16860"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HVT99179.1"
                     /translation="MKLFSELNLSQALQQQIAAANFVTPTPVQAGAIPPALEGRDVLA
                     TAQTGTGKTLSFLIPLIERMPKNAKGASALVLLPTRELAMQVLEAYRQLTHSKGTAAL
                     VCGGLNEGPQLDAIRRGARLIVATPGRLEDYLDRRLISLSGIKMLVLDEVDRMLDMGF
                     KPAIRRIAASLPADRQTLCYSATLSAEIRDVARDYLNDPVRIEIGSVLKPSPNVELQT
                     FEVPVDKKQELLEHLLGAHDGSFLVFVRTKHGADRVARRLKRAGHSAVPIHGDRTQSQ
                     RNSALRSFAEGHHRVLVATDVAARGIDVADVAHVVNFDMPKEAEDFVHRVGRTGRASA
                     RGMASTFAAPDETHTLRKMERTLAISTRKFRVRPRAAAHAHNA"
     gene            complement(5486..7102)
                     /locus_tag="VHE33_16865"
     CDS             complement(5486..7102)
                     /locus_tag="VHE33_16865"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HVT99180.1"
                     /translation="MKRWVKIAIGIGVVLVLVLVIVPFFVNADSFRPMLENQLSSALG
                     RKVTLGKLSFSLFSGSVVADQLTVADDPAFSAAPFLQAKSLKIGVDVGAFLFHHQVNV
                     RKFVAESPEIHLISNAQGVWNYASLGRTGTGTAPPQGPSPEVVVAIAQIDDGKVVVSS
                     SPAKGQPFVYDAVNITVDNLSSHSVMPFSVKANLPGSGSVSLNGTVGPFNEQDASATP
                     LSAQLAVRAFDPVKAGVLPASDGIDMQADVDAQLKSDGKTATSTGSIVAQHLLLAKGG
                     TPAPNPVHVTYTAQDDLRAQTGTVKDLAVATGPVTVHVTGTFATKEPATTVDLHVNAM
                     RVPVDAVEALLPAVGVHLPTGSQLKGGTLTAQLAITGTATSPVIEGPVEVDNTQLAGF
                     DLGSKIQGLKALTGTSGGTGIQVLHADVRQTSAQTALSNINCVVPAIGTATGQGTVAA
                     SGALNFQLTAKLSGSGAAGAAVDAASAALGGVAGNLLHTAANASVPMTITGTTSNPVI
                     RADVKAMMKGSAGTAAKKSLGNLLQGLMPK"
     gene            7425..>7723
                     /locus_tag="VHE33_16870"
     CDS             7425..>7723
                     /locus_tag="VHE33_16870"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HVT99181.1"
                     /translation="MEVTNAFIGKPGKPSEKDVTAALGASAPVWSEFVHWAEERGAAT
                     QEWKSYNVKHGWSLRLKRKDRNIVYLAPCSGCFQVSFILGEKAMDAVRHASFPPDV"
BASE COUNT         1450 a         2613 c         2189 g         1471 t
ORIGIN      
        1 cgatcccacg atcgatgtca tgccggttat tcctcttgat ctgacgggac aacccgaccc
       61 gaatgcaggc aagtcgatcg acgatctgat cgcccaggcg gagaagaacc gccccgaagt
      121 ggccatttac cagatgcagg ccgaagtgca gaggcaagcc ctcaaggaca ttaacagcga
      181 gttgctgcca aggttggata tgtatggcgt tttcgcgggc aacggcacag ccggcccggc
      241 gaatcccttt tgcaacctcg gaccccagta ttgctccacc accctgccca ctggctttgg
      301 gggtgagttc cagaacctgt tcaattacac ggcacctgaa taccaggtcg gtatgactct
      361 gcagatcaat ctgcgcaacc ggacgttcaa agccgatcag ttccgggcgg tgctgcagta
      421 ccggcaaagc cagatcgcat cggaacagca gcagaagagc atccgtttcg atgtgcgcaa
      481 ctcccagttc gctctcgaac aggctcaggc gcgggtggag gcggcacaga aatcccgcga
      541 tctggcccag catacgttcg acatcacgcg gcaggaacag cagctcgggg cgaaatccag
      601 cgccgatact ctgctgactg ccaatgctct ggccgtcgcc gagtcggctc tggatgccgc
      661 ccagtctgcg tatgagaagg cgaaagtgga tattgaccgg gccattgggg ataccctcaa
      721 ccggatgcag gtctcgatcg acgatgccaa atccggcgtg gttacgcacg ggccataatg
      781 gaagaagcta tcggctaaga gcgaatggct acaggctatg tccaccgcgg cccaacccgt
      841 catccctgaa gtttccggca gcaagctcgc gccgctggtt ctgatcgccg acgatcagcc
      901 ccatatcctc gacgctctcg agctgctcct tgagccggaa ggctaccgca tcgagaaggc
      961 cacctcgccc tacatggtgc tggacgcgct gaaatcctcg tccttcgatg cgctcctgct
     1021 cgacctgaat tacacgcgcg acacgacatc gggacgcgag ggcctcgatc tgctctcgca
     1081 aattcgtgcg catgagtcgc agatgccggt gatcgtgatg acggcctggg cgaacgtcga
     1141 gctggcggtt gaagcgatgc gccgcggggc atctgacttt gttcagaaac cctgggacaa
     1201 cgcgcgtctg ctgaccatcc tgcgcaccca gatcgacctg agcaacgccg tgcgccgcgc
     1261 ccagagactg gaagccgaga accgcctcct ccgcgccgcc aatctccccg agatgatcgc
     1321 cacggcggcc gtcatgcagc cggtgctcgc caccattacg cgcatcgggc cttctgacgc
     1381 gtccgtcctc atcaccgggg agcatggaac gggcaaggaa gtcgttgcac aaacgctgca
     1441 cgcactttcc cagcgtgctt cgcgctcgat ggtggccgtc aacaccggcg ccctccccga
     1501 gggcaccttc gaatccgaac tcttcggcca cgtcaaaggc gcctttaccg atgcccgcac
     1561 ggagcgcatc ggacgctttg agctggccga aggcggcacg ctcttcctcg acgaaatcgc
     1621 caacgtgccc ctgcggcagc aggcgaagct tctccgtgtt ctcgaaaccg gcgaaatgga
     1681 acgcgtcgga tcgtcaaaga cccggcacgt gaacgtccgc gtcatctccg ccacgaacgc
     1741 cgatatccgc aaggaagtcg atgccgggaa tttccgcgcc gatctgctct tccgcctcaa
     1801 cacggtcgag atccatctgc caccgctgcg cgaacgccgc gaggacattc caccgctcgc
     1861 cgcgcatttt cttgcccgct actcgcagcg ctatcgcaag ccggtatcgg gcatcgaccc
     1921 cacggcgatg gagctgatgc tccactacgc gtggccaggc aacgtgcgcg agctcgatca
     1981 caccatcgag cgcgccgtgc ttatgtcccg cgaagaggtt atcgaggtgc ccgatctcgg
     2041 cctgtcgccg gctcgagtct ccacccaggc tctcgaagag atgtctcttg agtcggtgga
     2101 gagtattctc atccgcaaag ccctctcgcg ctgcaacggc aacgtgagcc aggcggcaga
     2161 agccctcggt ctgagccgcg gggccctcta ccggcggatg gaaaagtatg gcatctagcg
     2221 gcgtcgattc tcacgcattc gcttacggct cctgcctcct tcctgctgtt ttctgcactc
     2281 cgcacgctgt accctgagcg ctgatggcga accaccgcgg cacatccagg cgtaagccca
     2341 aacgccagag cgtctccttc gaggcgcgcc tgcggatctt ctgcacggtc gccgcgctcg
     2401 tcttcatggc gctctgtgcg gcggtcctga ccctcctgca cgtcacggcg ggtcccatgc
     2461 tcgccacgct gggcattctg gggctcatcc tgctgctaat ttttgcggct tttatcgaag
     2521 aagccgtccg gcccgtgcaa acgcttgcca atgtggtcgc ggcactacgc gaggaggact
     2581 actctttccg ggctcgcggc gcgaatcgcg atgactcggt tggccagctt gctctcgaaa
     2641 tcaacgctct ggctgacacg ttgcagacgc agcgcgtcgc atcgctggaa gccgtcgccc
     2701 tgctccgccg cgtcatcgcc gagatggacg ctccggttct ggccttcgac caggagacgc
     2761 ggctccggct gctcaatccg gcggccgagc gcgtgttttc cctcgagccg gcgaagcatc
     2821 tcgggaagac cgccgaggat ctggccctca ccgagttgct ccaggaaacc agcgaaactg
     2881 tgcttcagct cgaaattcag cggccctccc ctctcggcgt gaagcgcacc gagcccagcc
     2941 agccggcgcg ctggatgatc cgccgcgggg aattccgcca gcgcggcgta ccccacacgc
     3001 tggtcgtgct ctccgatgtc agcgccgcac tgcgcgagga agagcgcctc gcatggcggc
     3061 gcctgattcg cgtcatcggt cacgagatca gcaattccct tactccgatc aagtcgattg
     3121 ccggttcgct gcgcacacgt atcgtgccga aatcggccaa tggctccgag cctcaggccg
     3181 aaccggcgcc cgctccgcca ccaccgattc cgccagccat ggccgttctg cagcgggacc
     3241 tgcagcgcgg cctttccgtc atcgagaacc gcgccgagtc gctgaaccgt ttcgtccagg
     3301 cctatcgcca gctggctcag ctgccttcac cggttctcag gcgggttccg ctgcagcctc
     3361 tgctggagcg agtggtgtcg ctcgaaattc gcctcgacgt acggctgctt tcggcgcccg
     3421 acgtggagtt gctggccgat acagaccaga tcgagcagct cctgatcaac ctcgtccgca
     3481 acgccgtcga gtcggcactg tcgtgtgtcg aggaagaacc ggaaccagcg cctgcgatcg
     3541 aagcaagcgc ggaaaatgca gcccggttcg ccgttcgccc gcgctcccag cccacgggaa
     3601 cgccgtgccg cccggaggtc acgatccgag caaccgaaga gagcgatctg gtgtgcatcg
     3661 ccatcgagga caatggcccg ggccttgcca atccggagaa cctctttgtt ccgctgtaca
     3721 ccacgaagaa atccggctcc ggggtcggtc tggcgctggc gcgccagatc gtcgaagccc
     3781 acggcggcat catcgagctg cgcaataagc ccaaccacgc cgggtgtatt gccgaagttc
     3841 ggattccgat cgccgagatc cctcgcactt agcggccagg agggttctgc tataatgctt
     3901 ttacgctcag ttatttggtt gtgagccgct ctcgccttca tccgcaagat cgtcctcccg
     3961 taatcctctc caggtccact cagcgtaatg gctcctgttg ccgatctcgg caaagggcca
     4021 ggctgacgcg ttcgaggctc atgcccagcg catttcagcc gaaaccatga gtgtgggtcg
     4081 aatctcgatc agcgcgtcat ctgcgcttcc cggattttcc tgcatagaaa ctgaaagctc
     4141 tccggcgcag ctccgcccaa tggcccgagc cgcacccgga acacaaacag ctccggccgt
     4201 cccacagccg gttccctgga tgaagagcag gcccaacggc ctcgcagagt ggaaccgcag
     4261 acgcccgagc caaggaatcc attgaaactg ttctctgaac tcaacctctc ccaggcgctg
     4321 caacagcaga ttgccgccgc caactttgtc acccccaccc cggttcaggc tggagccatc
     4381 ccgcctgcgc tcgaaggccg cgacgttctc gctacggcgc aaacgggcac cggcaaaact
     4441 ctcagcttcc tcatccccct cattgagcgc atgccaaaga atgcaaaggg agcttcagcc
     4501 ctcgtccttc tccccacgcg cgaactcgcc atgcaggttc tggaagcgta tcgccagctc
     4561 acccacagca agggcacggc ggctcttgtg tgcggcggcc tgaatgaggg cccgcagctg
     4621 gacgctatcc gccgcggtgc gcgcctgatc gttgccacgc caggccgcct ggaggattac
     4681 ctcgaccgca gactcatcag cctcagcgga atcaaaatgc tggttctcga cgaggtcgat
     4741 cgcatgctcg acatgggctt caaaccggcg atccgccgca tcgcggcttc cctgcccgcc
     4801 gatcgccaga ctctttgcta ttcggcaacc ctgagcgcgg agattcgcga tgtcgcgcgc
     4861 gactatctca acgatccagt ccgcatcgag atcggctcgg tgctcaagcc ttcgcccaac
     4921 gtcgagctgc aaacttttga ggttcccgtg gacaagaagc aggaactcct cgagcatctg
     4981 ctcggcgcgc acgacggcag ctttctcgtc ttcgttcgca ccaagcatgg agccgatcgc
     5041 gtcgcccgcc gcctgaaacg cgcagggcac tcggccgtgc ctatccacgg cgatcgcacc
     5101 cagtcccagc gcaactcggc gctgcgcagc ttcgccgagg gacatcaccg cgtcctggtt
     5161 gcgaccgacg tcgccgcccg gggcatcgac gttgccgatg tggcgcatgt ggtcaatttc
     5221 gatatgccca aagaagccga ggattttgtg catcgcgtcg gacgcacggg ccgagcttcg
     5281 gcgcgcggca tggcttctac cttcgccgcg cctgacgaaa ctcacaccct gcgcaagatg
     5341 gagcgcaccc tggcgatcag tactcggaaa ttccgcgttc ggccacgcgc ggcagcacac
     5401 gcgcacaacg cctgatccac aaaccatggc gatgcggagc gaagagcccg catcgccgct
     5461 ccgcctctgc tccctgatct tgtctttatt tcggcatgag tccctgcagc agatttccca
     5521 ggctcttttt cgccgctgtg ccggcgcttc ccttcatcat tgctttcacg tcggcgcgga
     5581 tcaccggatt cgacgtagtc cctgtgattg tcatcggcac gctggcattg gctgctgtgt
     5641 gcagcaggtt gccggcgacg ccgcccagag ctgccgacgc tgcatccacg gcggcccctg
     5701 ctgcgcctga accgctcagt tttgcggtca gctgaaaatt cagtgcgccc gacgcggcca
     5761 ctgtcccctg accggttgcg gtgccaatgg ccggaaccac gcaattgata ttggacaggg
     5821 ctgtctgcgc agaagtctgg cgcacatcgg catgcagaac ctggattccc gtcccgccgc
     5881 tggtccccgt caacgccttc agtccctgaa tcttcgaccc cagatcgaat cccgccagct
     5941 gcgtattgtc cacttccacc ggcccctcaa tcacgggaga cgtcgccgtc cctgtaatcg
     6001 caagttgcgc cgtcaaagtc ccgcctttga gctgcgaccc cgtcggcagg tgtacaccca
     6061 ccgctggcag cagagcctcc actgcatcca ccggcactcg catcgcgttg acatgcagat
     6121 ccacggtggt tgccggttct ttcgttgcaa atgttcctgt aacgtgcacg gtcacgggtc
     6181 cggtcgccac cgcgagatcc ttcacggtgc ccgtctgcgc tctcagatcg tcctgggcgg
     6241 tgtacgtcac gtgcaccgga ttcggcgccg gggtgccgcc tttcgccagc aacagatgct
     6301 gcgccacgat gctgccggtg ctggttgcgg tctttccgtc ggatttcagc tgggcgtcga
     6361 cgtccgcctg catgtcaatt ccgtcgcttg ccggcagcac cccagccttc accggatcga
     6421 aagcgcgcac tgccaactgc gcactcaatg gcgttgccga cgcatcctgc tcattgaacg
     6481 gtcccacggt gccgttcaac gacaccgatc cactgcccgg cagattcgct ttcaccgaga
     6541 aaggcatgac cgaatgcgat gacagattgt cgaccgtgat gttgacggcg tcatagacga
     6601 aaggctgtcc tttcgccggc gatgaagaca cgacgacttt gccgtcgtcg atttgtgcga
     6661 tggccacgac cacttccggt gagggaccct ggggtggggc ggtgccggtt cctgttcggc
     6721 ccaggctggc gtaattccac actccctgcg cattggagat caggtgaatc tcaggcgact
     6781 ccgcaacaaa ctttctgaca ttgacctggt gatggaacag aaacgcgccg acatcgacac
     6841 cgatcttcag ggacttcgcc tgaagaaaag gcgcagcgct gaaggccgga tcgtcggcca
     6901 cggttagctg atccgccacc acgctcccgg agaacagtga aaagctcagc ttgcccagcg
     6961 tgactttccg gcccagagcg ctcgacagct gattctcgag catcggccgg aaggaatccg
     7021 cgttgacgaa aaacggcacg atcaccagca ccaacaccag gaccactcca atgccgatcg
     7081 caatctttac ccagcgcttc atagcttctc cgccctcgtt tctgatctga gatgcagtcg
     7141 ccccttaatt tactccctgc cataccattc gatggaacat tatgtccatg gcctttggcg
     7201 attccttcgc gccgcgattg atcaccagcg cactaacgcc agcctacttt ggcgcggtca
     7261 agcacctcct gtaccgtgat cgcgtcaatc gatttctcaa ggatgagctg gatcagcgga
     7321 ccacacgcag gtcagtttga tcaagctaaa cacaccggcg gtgcacctct gcttcggggc
     7381 gtagaatttc ccgttcctct gctgacgatt tgtggaggtt caccatggaa gtcacgaacg
     7441 ccttcatcgg caaacccggg aagccatccg aaaaagacgt caccgccgcc ctcggcgcca
     7501 gcgctcctgt ctggtccgag ttcgtccact gggcagaaga gcgcggtgcc gccactcagg
     7561 aatggaaatc gtacaacgtg aagcacggat ggtcgctccg cctgaagcga aaagatcgca
     7621 acattgttta tctggcgccc tgcagcggct gtttccaggt gtcgttcatc ctgggcgaga
     7681 aagccatgga tgccgttcgt cacgccagct tcccacctga tgt
//
LOCUS       DASZYN010000653         1583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1001864, whole genome shotgun sequence.
ACCESSION   DASZYN010000653 DASZYN010000000
VERSION     DASZYN010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001864"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VHE33_16875"
     CDS             <1..298
                     /locus_tag="VHE33_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99182.1"
                     /translation="LKQTTHNLNEITASINSGKGTLGMLTKDPKTAAKLSDTIEKLDT
                     MLTQINEGQGTIGALYKDRRLYDHVDHLMTDSSDLVQAIRKDPKKYLNIRVHIF"
     gene            819..>1583
                     /locus_tag="VHE33_16880"
     CDS             819..>1583
                     /locus_tag="VHE33_16880"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HVT99183.1"
                     /translation="MRIVRSAWRYAVPLLFILPAGLCAQQLVSTSDTSQAPTEPKALH
                     SFDLNAIDKSVDPCVDFYQYACGNWRKENPIPADQSRWGRFNELNERNRYLLYVDLKK
                     AADSPKTALQRKYGDFFAACMDTDQANKLGDKPVQPLFARIDAMKDKKEIAGVAGYID
                     SKTPTGIFFRFGVEQDQKDSTKQIEGLHQGGLTLPDRDYYLQDNANMQEIRAKYHDYI
                     VAVLKLTGESEDQAKADADHVMEIETALAKGSTPRVD"
BASE COUNT          356 a          449 c          470 g          308 t
ORIGIN      
        1 gctgaagcag actacccaca acctgaatga gatcaccgca tcgatcaact caggcaaagg
       61 cacgctgggc atgctgacca aggatccgaa gacggcggcc aaattgtcgg acaccattga
      121 aaagctcgac accatgctca cccagatcaa cgagggtcag ggaacgatcg gcgcactgta
      181 caaggatcgc aggctctacg accacgtgga ccatctcatg acggactcga gcgatctggt
      241 gcaggccatc cggaaagatc ccaagaagta cctgaacatc cgcgtacata ttttctagct
      301 tgtggttttc tggttcattg aacaatgagg cttccgccgg gaaggcctca ggggctgaag
      361 cccaccgggg tttgcgttct ttgcggcacg actgaagtcg tgccctgata caaaagccgg
      421 acgatttttg aacgaatctt cgggacacca cgcccgtggg ttgttgcgag atctcttcgt
      481 ggggaagaga agcaggttcc tcgatccgtt gtggggacaa ggggaagcag gtccctccgc
      541 tccgctgcgc ttcggtcggg atgacaagtg tgggtgttgg tgcagggaag caggtcccgc
      601 cgcttcccct tcgccaggct cagggttcgg tcgggatgac aagggtgggt gttggtgcag
      661 gaaaacaggt cctttcgctt cgtcgggatg acaagcagat tcctctgctt cgggcggaat
      721 gacaagggga agaaacttca gaaacgccac acagcgttta tttctttcgt gcggctgcgg
      781 tacgctatcc gctgaacgct gttttcctga ggagtcccat gcgcatcgtt cgctctgcct
      841 ggcgctatgc cgttcccttg ctgttcatcc ttcccgccgg cctttgcgcg cagcagctgg
      901 tttccacctc cgacacatcg caggcgccca ccgagcccaa agctctgcac agcttcgatc
      961 tgaacgcgat cgacaagagc gtcgatccct gtgtcgactt ctatcaatac gcgtgcggca
     1021 actggcggaa ggagaatccc attccggcgg atcagtcgcg gtggggacgc ttcaacgaac
     1081 tcaacgaacg caaccgctac ctgctctacg tcgatctgaa aaaagcagcg gattctccaa
     1141 agacggcgct gcagcgtaaa tacggcgact ttttcgcggc gtgcatggac accgatcagg
     1201 cgaacaagct gggggacaag ccggttcaac cgctctttgc gcgcatcgat gccatgaagg
     1261 acaagaagga gatcgcaggg gtcgccgggt atatcgactc gaagactccc accggcatct
     1321 tcttccgctt cggggttgag caggatcaga aggattcaac caaacagatt gagggcctcc
     1381 accagggcgg gctgactctg cccgatcggg actactacct gcaagacaac gcgaacatgc
     1441 aggagatccg cgccaaatat cacgactaca tcgtggcggt gctgaagctc accggcgaat
     1501 cggaggatca ggcgaaggcg gacgccgatc acgtgatgga aatcgagacg gcgctggcga
     1561 agggctcgac gcctcgggtc gat
//
LOCUS       DASZYN010000654         4005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_486690, whole genome shotgun sequence.
ACCESSION   DASZYN010000654 DASZYN010000000
VERSION     DASZYN010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486690"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VHE33_16885"
     CDS             complement(<1..201)
                     /locus_tag="VHE33_16885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99184.1"
                     /translation="MAEVTPRARKMTIRRRLKAASRKVREVSLVAHALASTRHTVMAQ
                     IVPMRRCNLSCAYCNEYDDVSKP"
     gene            complement(508..987)
                     /locus_tag="VHE33_16890"
     CDS             complement(508..987)
                     /locus_tag="VHE33_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99185.1"
                     /translation="MPQRALILAVCALLCGTAWAAQVPSFRRPPMPEFQRATFLGASP
                     CKPDPYYANCEHRGDPLYTIVLDGRTFVLHPGFSLPLLTMAHLLLTPWNRSSLSLPGD
                     NVLDHLPPGTMVQIRMRDGGMDVRVLAQSSKGPRYLASHYGFAKSQDEANSGDGPSS"
     gene            complement(1226..2128)
                     /locus_tag="VHE33_16895"
     CDS             complement(1226..2128)
                     /locus_tag="VHE33_16895"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HVT99186.1"
                     /translation="MNTFLRPVVKFLPIAVVTAAALSLPHVVHGQQGLLGGAFPPSGA
                     GPAEQHVFPPGQDPAIASASQGYLGVGLHDVDSDRAAQLKLKDVRGVEVTTIDHDAPA
                     AKAGLKLHDVILGMNGQTIVGQAQLTRLLHDTPAGRSVTLLISRDGQQQTLSVQLADR
                     ATIEANAWSQHIPVPDPDSDIALPANTGNSYGNGIFSSLTSNPFYTGLDLDMLGPQLA
                     NFFGVHDGQGLLVKRVDDNSPAAAAGLRAGDVITRVNGENVATTSQWVHDIHANRGKP
                     IQLTVIREKHEGTLSMVAGRPKKS"
     gene            complement(2232..3209)
                     /locus_tag="VHE33_16900"
     CDS             complement(2232..3209)
                     /locus_tag="VHE33_16900"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HVT99187.1"
                     /translation="MNCNLAQEHIVLSVYGELPDDRAHQLEQHLAQCEPCRQEMEAIS
                     ALHKAMSVAPVVEPSPSLLARTRLRLDEALDALPQASFFRRLSQRIHRGFGALQGAPV
                     MATVVLLAGGALGAWGGYRVAGHNVRATSTVAQNSPAPLPILSDPDSAQIGGVSSISI
                     EPDSENVEVHFQRVIPETAFGTLDDPQIRQLLVLAARNYANQVVHDDSVNLLAKACRA
                     GRECSGGAVRNTLMVALRYDKDARVRRKALEGLESYIPVDTRVRDAVLEAVLHDSNAD
                     VRAQAISLLEPVDADSAVREVLQTVANRDQDQRIRSVSRQYLEQVSQIQ"
     gene            complement(3292..3888)
                     /locus_tag="VHE33_16905"
     CDS             complement(3292..3888)
                     /locus_tag="VHE33_16905"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVT99188.1"
                     /translation="MGGPVRENLHETDLIREAQAGSRTAFDALVRQYDQAVLRLAMHL
                     TGSEQDAQDIQQEAFLKAYRYLGNFRFECSFYTWIYRIVTNLCLDHLRRRKSRREDQA
                     VVIDSSGEEMDLLTNVSDDRSGANPGRELDRKYLGHRIMMALDELTPRERMVFELKHY
                     QGLRLRTIGEMLNTTEETAKNTLFRATKKLRAKLAELR"
BASE COUNT          724 a         1006 c         1497 g          778 t
ORIGIN      
        1 gggtttcgag acgtcgtcgt actcattgca ataagcgcag gagagattgc accggcgcat
       61 gggcacaatc tgggccatga cggtgtggcg cgtggaggcg agggcatggg cgacgaggga
      121 gacttcccgc accttgcgcg aggcagcttt gaggcggcgg cggatcgtca ttttgcgggc
      181 cctgggggtg acttctgcca tgtcttttct attgtagcgg gtgggggagg acggcgttcc
      241 gttgtcggcg ttcagcgttc agtaagagcg gcgcgattcc cattttcccg cgcgttccca
      301 catctgccaa aaccgggcag atgtggtacc cgttgggtcg tgccttgtta caaaaccggc
      361 tgattgagat cctcgttccc acatctgcca aaaccgggca gatgtggggc acccgttggg
      421 tcgtgccttg ttacaagaca ggctcattga gattttcgtt cccacatctg ccgaagcggg
      481 cagatatgga cacccgtgga gcctgggtta cgaggatggg ccgtcgccgg agttcgcctc
      541 gtcctgggat ttggcgaagc cgtagtgcga ggcgagatag cgcgggccct tcgaagattg
      601 ggcgaggacg cgtacgtcca tgccgccgtc gcgcatgcgg atctggacca tggtgccggg
      661 aggcaggtgg tcgaggacgt tgtcgccagg cagggaaagt gaggaacggt tccagggggt
      721 aaggaggagg tgcgccatgg tcagcagcgg aaggctgaac cctggatgca ggacgaaggt
      781 ccgtccgtcg aggacgatgg tgtagagcgg atcgccgcgg tgttcgcagt tggcgtagta
      841 gggatccggc ttgcaggggc tcgcgccgag gaaggtggct cgctggaatt cgggcatcgg
      901 ggggcgacgg aaggagggca cctgggccgc ccaggcagtg ccgcagagca gtgcgcagac
      961 ggcgagaatg agagcgcgct ggggcacgcg gggagtgtag cagaaagacg gcgtgcagcg
     1021 tttggcggac agggtttgtg agtttggagt ttggagtttg gagttgagag ttgtgagttg
     1081 tgagttgaga gttgtgagtt tggagttgag agtagagcgg ttcggttcag agtgcgatag
     1141 gccgtcggtc tgacccgatc aacggtatcc gctacccgct ccgctatgcg ctacccgcta
     1201 tccgctaaac gctcaacgct gctgttcagc tctttttggg gcggccggcg accatgctga
     1261 gggtgccttc atgcttttcg cggatgacgg tgagctggat tggcttgccg cgattggcgt
     1321 ggatgtcatg gacccactgg ctggtggtgg cgacgttctc gccgttgacg cgggtgatga
     1381 cgtcaccggc gcgcaggccg gcggcggctg cgggactgtt gtcatcgacg cgcttgacga
     1441 ggagtccctg gccgtcatgg acaccgaaga agttggcgag ctgagggccg agcatgtcga
     1501 gatcgagacc ggtgtagaag ggattcgagg tgagcgagga gaagatgccg ttgccgtagc
     1561 tgttgccggt attggcgggc agagcgatgt cgctgtcggg gtcgggcaca gggatgtgct
     1621 gggaccaggc attggcctca atggtggcgc ggtcggcgag ctggacggac agggtctgct
     1681 gctgtccgtc gcggctgatg aggagggtga cgctgcggcc ggcgggcgtg tcgtggagca
     1741 agcgcgtgag ctgagcctga ccgacgatgg tctggccgtt catgccaagg atcacgtcgt
     1801 gaagcttcag gccggctttg gcggcgggcg cgtcgtggtc gatggtggtg acttcgacgc
     1861 cgcgaacatc cttcagtttg agctgggcgg cacggtcgga gtcgacgtca tgcaatccca
     1921 cgcccaggta tccctgggaa gcgctggcga tggcaggatc ctggccggga ggaaagacat
     1981 gctgttcagc gggaccagca ccggagggag ggaaagcgcc gccgaggagg ccctgctggc
     2041 cgtggacgac gtggggcagg gagagagcgg cggcagtgac gacggcgatg gggagaaact
     2101 ttacaactgg cctcagaaaa gtgttcatct tgcgcctcga atctgtcctg ttgtctctga
     2161 cgaacgagtg ctgcgctggt caaccgcagg ctgcccggga acatagtacg caaggggcag
     2221 cctgcttgtc ttcattgaat ctgtgaaacc tgttcgagat actgccggga aacgcttcgg
     2281 atgcgctggt cctgatcacg attggcgacg gtctgcagga cttcgcggac ggcggagtcg
     2341 gcatcaacgg gttcgagaag gctgatggcc tgggcgcgga cgtcggcgtt ggagtcgtgc
     2401 aggacggctt cgaggacggc atcgcggacc cgggtgtcga ccgggatata ggactcgagg
     2461 ccttcgaggg ccttgcggcg gacgcgggca tccttgtcat agcggagggc gaccatgagt
     2521 gtgttgcgaa cggctccgcc actgcactcg cgaccggctc ggcaggcttt ggcgagcaga
     2581 tttacagaat catcgtggac gacctggttg gcataattgc gggcggcgag gacgagcagc
     2641 tggcgaatct gcgggtcgtc gagggtgccg aaggcagttt cggggatgac gcgctggaaa
     2701 tgaacttcga cgttctccga gtcgggctcg atcgagatgc tgctgacgcc gccgatctgg
     2761 gccgaatccg ggtcagacag gatcggcagg ggggctgggg agttttgcgc gacggtcgag
     2821 gtggcgcgaa cgttgtgtcc agcgacgcga tagccgcccc aggcgccgag ggcaccgccg
     2881 gcgaggagca cgaccgtggc catgacaggc gcaccctgca gggcgccgaa gcccctgtgg
     2941 atgcgctgac ttagacggcg aaaaaagctg gcctggggga gggcgtcgag ggcttcatcc
     3001 agccgcaggc gggtacgcgc gaggaggctg ggggagggtt ccacgacagg agcgacagac
     3061 atggccttgt ggagcgcgga gatagcttcc atttcctgac ggcaaggctc gcactgggca
     3121 agatgttgtt cgagctggtg ggcccggtcg tcgggcaact ctccataaac ggacaggacg
     3181 atgtgttcct gtgccagatt gcagttcata cgtacgtgcc tcacttcgcc tcaaagtttt
     3241 ggacgtccaa aatcagcgtt tagcgtctag cgcctagctt ttagttattc gttaccgcag
     3301 ttcggcgagc tttgcccgca gttttttggt ggcacggaag agggtattct tggctgtttc
     3361 ttccgtggta ttgagcattt ctccgatggt gcgcaggcgg agcccctggt agtgcttgag
     3421 ttcaaagacc atgcgctcac ggggggtgag ctcgtcgaga gccatcatga tgcggtggcc
     3481 gaggtatttg cgatcgagct cgcggccggg attggcgccg gagcgatcgt cggagacatt
     3541 ggtgagcaga tccatctcct caccggagga gtcgatgaca acggcctgat cttcgcggcg
     3601 ggacttgcgg cggcgaaggt gatcgaggca gaggttggtg acgatgcggt agatccaggt
     3661 gtagaaggag cactcgaaac ggaagttgcc gaggtagcga taggccttga ggaaggcctc
     3721 ctgctgaatg tcctgggcat cctgttcgga accggtgagg tgcatggcga gccgcaggac
     3781 agcctggtcg tactgacgca cgagagcgtc gaaggcagtg cgggagccag cctgggcctc
     3841 acggattaaa tcggtctcgt ggaggttctc ccgaacgggc ccacccatac gggtggattg
     3901 tattcgaggc cgggaggggg atggccgcgc tgaagacaaa aaccgaagca catcagcagg
     3961 cattagggct cctgggacgt gggttgcgaa gactacgcac agcta
//
LOCUS       DASZYN010000655         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_229739, whole genome shotgun sequence.
ACCESSION   DASZYN010000655 DASZYN010000000
VERSION     DASZYN010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229739"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VHE33_16910"
     CDS             complement(<1..453)
                     /locus_tag="VHE33_16910"
                     /inference="COORDINATES: protein motif:HMM:NF013216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HVT99189.1"
                     /translation="MHRPPQPGRRQDPSRRGSQDTVAPARRISYRILLQMVTTDAHSD
                     DLLRSPEVDALSTQDRALTTTLVLGTLRWQLALDEQIRALLSRPEMQLSPEAATSLRM
                     GAYQLQFLDRVPAHAVIHDAVELIKHGKERGAAGMVNAVLRKMQRSGTG"
     gene            complement(516..1454)
                     /gene="fmt"
                     /locus_tag="VHE33_16915"
     CDS             complement(516..1454)
                     /gene="fmt"
                     /locus_tag="VHE33_16915"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HVT99190.1"
                     /translation="MKLVFCGTPVFAVPTLKLALRSGYDVPLVVTQPDRPVGRDQVMT
                     APPVKTTAIEAGIRVTQPEKIKNNAEFRAELEAIQPDAILVVAYGRIIPKWMLDLPRL
                     GNLNLHGSLLPKYRGAAPVQWAIANGEGSTGVTTMRLDEGLDTGDILLQREMALAPSM
                     TAVDVFPLLAAMGADLMIETLRGLDAGTIAPRKQDESKATLAPILTRDDGRMDFAQPA
                     MTLYNRWRGFQPWPGAWTMLDGKKLAVHRMLPTEVRGGRSRPPGEILVEQGTMFAACG
                     DATWLELVEVQAEGKRRMTAGDFLRGHNLQSGIRLG"
     gene            complement(1451..1963)
                     /gene="def"
                     /locus_tag="VHE33_16920"
     CDS             complement(1451..1963)
                     /gene="def"
                     /locus_tag="VHE33_16920"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HVT99191.1"
                     /translation="MIREIVKYPDPILQRPTEKVTEFNDELRTLADDMFESMYKAIGI
                     GLAAPQIGVGKRITVIDLSNQKNPKEKIVLVNPEIIHKEGKQVEEEGCLSLPEIRDKV
                     SRAMKVKVKAQDLEGKWFEMDGEELLARAFQHEIDHLDGILFPWRMSALKRDLILRKI
                     RKMQKSGEWK"
BASE COUNT          349 a          686 c          567 g          422 t
ORIGIN      
        1 gccagtccct gaacgctgca tcttccgcag aaccgcgttg accatgcccg cggcgccgcg
       61 ctcttttccg tgcttgatca gctcgactgc atcgtggatc acggcatgcg ccggcacacg
      121 atccagaaac tgcaactgat acgcgcccat ccgcaggctc gtcgccgctt ccgggctcag
      181 ctgcatctcg gggcgcgaca gcagcgcccg aatctgctca tcgagcgcca gttgccagcg
      241 cagcgtgccc agcaccaggg tcgtggtcag cgcgcgatcc tgcgttgaca gcgcatccac
      301 ctccggcgac cgcagcagat cgtcactgtg ggcgtcggtg gtgaccattt gtaacaggat
      361 tcgatacgaa atccgccgcg ccggcgcaac ggtgtcctga gaacctcgcc gcgaaggatc
      421 ctgtcttcgt cctggttgtg gcggtctgtg cattttctaa acgttgacac ggaacgctgt
      481 tcggaacagc cgagtgcgga ttgccgaacg ccgttctacc ccagtcgaat cccggactgc
      541 agattgtgcc cgcgaagaaa atcgccggcc gtcatcctgc gctttccctc cgcctgcacc
      601 tcgaccagtt ccagccaggt agcgtcgccg caggccgcga acatcgttcc ctgctctacc
      661 agaatctctc cggggggacg cgacctcccg cctcgcacct cggtcggcag catgcgatgc
      721 acggcgagtt tcttgccgtc cagcatcgtc cacgcccccg gccacggctg aaatccgcgc
      781 caccggttgt agagcgtcat tgccggctgc gcaaaatcca tgcgtccgtc gtcgcgcgtc
      841 aggatcggcg ccagcgtcgc cttcgactcg tcctgcttcc gtggcgcgat tgtgccggcg
      901 tccaggccgc gcagcgtctc gatcatcaga tccgcgccca ttgcagccag cagcggaaac
      961 acatccacgg ctgtcatgct gggcgccagg gccatctcgc gctgcagaag aatgtcgccg
     1021 gtgtcgaggc cctcgtcgag gcgcatggtc gtcacgcccg tcgacccttc gccattggcg
     1081 atggcccact gcaccggcgc ggctccgcga tacttcggga gcagcgagcc atgcaggttg
     1141 agattcccga gccgcggcag gtcgagcatc cacttcggaa tgatgcgccc ataggcgacg
     1201 acgagaatcg catcgggctg aatggcttcg agctcggcgc gaaactccgc attgtttttg
     1261 attttttccg gctgcgtaac gcgaataccg gcttcgatcg cggtcgtctt caccggcgga
     1321 gccgtcatca cctggtcgcg tcccaccggg cgatccggct gcgtgaccac cagcgggaca
     1381 tcgtatccgg aacgcagcgc cagcttcagc gtgggcaccg caaacaccgg cgtcccacag
     1441 aacaccagct tcatttccac tcgccggact tctgcatctt gcgaatcttc cgcaaaatca
     1501 ggtcccgctt cagcgcgctc atccgccagg gaaacaggat tccgtcgagg tgatcgatct
     1561 cgtgctggaa ggcccgtgcg agcagttctt cgccgtccat ctcgaaccac ttcccctcga
     1621 ggtcctgggc cttcaccttc actttcatcg cgcgcgatac cttgtcgcga atctcgggca
     1681 ggctcaggca cccttcctct tccacctgtt tgccttcttt gtgaatgatc tccggattca
     1741 ccaggacgat cttctctttg ggatttttct gattgctcag gtcgatcacc gtgatgcgct
     1801 tgccgacgcc gatctgcggc gccgcgagcc caatcccaat ggccttatac atagactcga
     1861 acatgtcgtc ggccagggtg cggagctcgt cgttgaactc ggtcaccttt tcggtcggcc
     1921 gctgcaaaat cgggtccgga tatttaacaa tttcgcgaat catgttgtgc ttcgtgtccc
     1981 aaattctttt gaatgcagcc aacagcggtt agcgaaaagc tttt
//
LOCUS       DASZYN010000656         3821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_165485, whole genome shotgun sequence.
ACCESSION   DASZYN010000656 DASZYN010000000
VERSION     DASZYN010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165485"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1540
                     /locus_tag="VHE33_16925"
     CDS             206..1540
                     /locus_tag="VHE33_16925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT99192.1"
                     /translation="MRLTGYPASAPARPSHGVRPIHRDAGISVIEECAPEPYREHDAR
                     QYVDRDPEEYSPGQLQVIEVDFRRDGRWIDFLSSHPDALIYHHPGWLSALEAEYRGKC
                     IALACEANDGSFEGILPLLSTRGFALPWGRNQVGRRLSSLPRTPLAGPLVTHRAALAA
                     LLKAAVDRVMSRPGLQLELKTTLPGLDELVPELECIRWRDTYVRGLPANDDPDLRLNA
                     TGRRTTRSCELCEKCHARSFGNSRQNHQVRWAVNKARKQGLSIRGVASEHDLKHWYRL
                     YLKVMRRNAVPPRPFRFFQHLWQQLEPLGHMELVVADSGETGMLSGSILLRFGHSVFW
                     AFTGCNEDGFLHHANDLTLWHCLQESCSGGYSRFDLGEVAESHPELAQFKAKWGTTLQ
                     PLYRYYFPPCRPSESVEEDLAHNRLAGYVGQVWRRLPLSVVAALGDWVFSYL"
     gene            complement(1619..3229)
                     /gene="gpmI"
                     /locus_tag="VHE33_16930"
     CDS             complement(1619..3229)
                     /gene="gpmI"
                     /locus_tag="VHE33_16930"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HVT99193.1"
                     /translation="MPVSKKPVVLTILDGWGYRPQIENNAIALARKPAYDKLLKEYPN
                     TLLHASDHFVGLPDGQMGNSEVGHLNIGAGRIVQMDITRIDALIANGDFFTHPTIVEA
                     MQKARKTQLHLFGLVSDGGVHSHQEHLYALLKTAKQQGVAKVFVHAFMDGRDTLPTSG
                     AGYIAALEQKMREYGGKLASVSGRYYAMDRDRRWEREKKAFDAMVKGHAEGGAYKDAV
                     ARIKESYNNGITDEFIVPFVVTDEHGHPNGLIRDEDVCIMFNYRADRARQITRVLARN
                     SGLSKEGGRELPSWEELDATIPRSEIPSRLHYVCMTQYDKNFTLPMIILPESMENLLA
                     NLMAQANLRNLRVAETEKYAHVTYFFNGGIETPFPGEDRILIPSQKVATYDLAPEMSA
                     AGIADTVIKAINDTAFDVIVVNFANADMVGHSGKLEPTIRAVETVDGCLGQIYQALRQ
                     RGGGAWLITADHGNAELMVDPVTGGPHTAHTTNPVPFIFVSEDAQHYSLRPDGSLRDI
                     SPTILGLLGLPQPRQMTGGDLRVPKGRG"
     gene            complement(3309..>3821)
                     /locus_tag="VHE33_16935"
     CDS             complement(3309..>3821)
                     /locus_tag="VHE33_16935"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HVT99194.1"
                     /translation="LGSMPLLAANTGKPGSARAVASTQAENVRPAHPVTAAQVYEILE
                     LTGTNTQKREMLDGLLPHLKEIMPWMPADVVGDLQRTLGTADFEGAMVRSFQQRLSTE
                     EAAQILAFYKSPAGRHMIAVMPAVLNEGQDAVAELGQQVMVEVIQRHKDEIDAAAKLY
                     HEEHPQKPQP"
BASE COUNT          682 a         1203 c         1133 g          803 t
ORIGIN      
        1 gaaattctcc tggaccgcga tcgcagacca gacgatcggg ctgtatcagg aattgatcgc
       61 gagccacagg cgcgtttagc ccctgcagct tcttctccac gcaaattgtg acggcgatca
      121 ccagcatcac gcaaggcatg actatggcca ccatcatgcc ccttccgttg cattgtgact
      181 cgactccgcg cgaattaccc tggccttgcg actgaccggc tatccggctt ctgctccagc
      241 cagaccttcg catggagtaa gacccatcca tcgcgacgca gggatttccg ttatcgagga
      301 atgcgcgccc gagccatacc gggagcacga tgccaggcag tatgtggacc gggatccgga
      361 ggaatattcg ccggggcaac tgcaggtgat cgaggttgat ttccgccgcg acgggcgctg
      421 gatcgacttc ctctcctcgc atccggatgc attgatttat caccaccccg gctggctgtc
      481 ggccctggaa gccgagtacc gaggcaaatg catcgcgctg gcctgcgagg ccaacgatgg
      541 atcattcgaa ggaatcctgc ccttgctgtc gacccgcgga ttcgctctac cgtgggggcg
      601 gaaccaggtg ggaagacgcc tgtcgtcgtt accccgtacg ccgctggccg gaccgctggt
      661 gacgcatcgg gcggctctgg ccgctcttct taaagctgcc gtggaccgcg tcatgtcgcg
      721 gcccggtctg caactggaat tgaaaacgac gctcccgggc ctggacgaat tagttccgga
      781 gcttgagtgc attcggtggc gcgataccta tgtccgtgga cttcccgcga atgacgatcc
      841 cgatctccgg ctgaatgcaa ccgggcgccg caccacccgg tcgtgcgagc tgtgcgaaaa
      901 atgtcatgcc cggagcttcg gaaattccag gcaaaaccat caggtgcgat gggcggtcaa
      961 caaagcgcgc aagcagggcc tctctatccg tggcgtagcc agtgagcatg atctgaagca
     1021 ctggtaccgg ctctatctga aggtgatgcg gcgcaatgcc gtgcctccac ggcccttccg
     1081 attcttccag catttgtggc agcagttaga accgctgggc catatggaac tggtggttgc
     1141 cgacagcggc gagaccggga tgctcagcgg atccatcctg ctgcggtttg ggcacagcgt
     1201 cttctgggct ttcaccggct gcaacgaaga cggtttcctt caccatgcca acgacctgac
     1261 gctctggcac tgtcttcagg aatcgtgcag cggaggatac agccggtttg acctgggcga
     1321 agtggcggag agccatccgg aactggcgca attcaaagcg aaatggggca cgaccctcca
     1381 accactctat cgatactatt ttccgccatg ccgtccgtcg gaatcggttg aggaagacct
     1441 ggcccacaac cggctcgcag gatacgtcgg ccaagtgtgg cgccgcctgc cgctctcggt
     1501 cgtcgctgcc ctaggagact gggttttcag ttatctttga tcggccggtc cggaaaatgg
     1561 cccggctcca gaggggagcc gggccttttc ctgttgacta ttggcgattg gcgattccct
     1621 atcccctgcc cttcggcact cgcaagtccc cgccggtcat ctgcctcggc tgaggcagcc
     1681 ccagcagtcc aaggatcgtc ggcgaaatat cgcgcagcga accatccggg cgtagcgaat
     1741 agtgctgcgc gtcctcgctg acgaaaataa acggcaccgg attggtggtg tgcgccgtgt
     1801 gcggtcctcc ggtcacggga tcgaccatca gttccgcgtt gccgtgatcg gcggtgatca
     1861 gccacgctcc tccgccgcgc tggcggagcg cctgatagat ttgccccagg cagccgtcga
     1921 cagtttcaac cgccctgatc gttggctcca gcttgccgga gtgtccgacc atgtcggcgt
     1981 tggcaaagtt cacgacgatg acatcgaatg cggtgtcgtt gatggccttg atcaccgtgt
     2041 cggcaatccc ggccgccgac atctccggcg ccagatcgta ggtcgcgacc ttctgcgacg
     2101 gaatcagaat gcgatcctct cccggaaacg gggtttcgat gccgccattg aagaagtacg
     2161 tcacgtgggc gtacttctcc gtctccgcca cgcgcaggtt gcggagattt gcctgcgcca
     2221 tcaggttggc cagcagattc tccatcgatt ccggcaggat gatcatgggc agtgtgaaat
     2281 tcttgtcgta ctgggtcatg cacacgtagt gcagccggct gggaatctcg ctgcgcggaa
     2341 tggtggcgtc gagttcttcc caggacggca gctcgcgtcc gccttctttg ctcagcccgc
     2401 tgttgcgcgc cagcacgcgt gtgatctggc gggcgcgatc ggcgcggtaa ttgaacatga
     2461 tgcagacgtc ttcgtcgcgg atcagaccgt tgggatggcc gtgctcgtcg gtgacgacga
     2521 aaggcacgat gaattcgtcg gtgatgccgt tgttgtagga ctccttgatg cgcgcaacgg
     2581 cgtctttgta cgcgccgccc tcggcgtggc ccttcaccat ggcgtcaaag gctttcttct
     2641 cgcgctccca gcggcgatcg cgatccatcg cgtagtagcg gccggaaacc gaggccagct
     2701 taccgccgta ctcgcgcatc ttctgttcca gcgcggcgat atagccggct ccgctggtgg
     2761 gcagcgtgtc gcgtccgtcc atgaaggcgt ggacgaagac cttcgcgacg ccctgctgct
     2821 ttgccgtctt caacagcgca tagagatgct cctgatgcga gtgcacgccg ccatcagaaa
     2881 ccaggccgaa cagatgcagc tgggtcttcc gcgccttctg catggcctcg acgatggtcg
     2941 gatgcgtgaa gaagtcgccg ttggcgatca gcgcgtcgat ccgcgtgatg tccatctgga
     3001 cgatgcgccc ggcgccgatg ttcaggtgac cgacttcgct gttgcccatc tgcccgtcgg
     3061 gcagccctac gaaatgatcg gaagcatgca gcagcgtgtt ggggtactcc ttgaggagtt
     3121 tgtcgtaggc cggcttgcgc gccagcgcga tagcgttgtt ttcaatctgg gggcgatagc
     3181 cccagccatc cagaatggtc agaacaacag gcttcttcga aacaggcaca caatctcctc
     3241 tgtacgtctt cttttcgcac acacgaaaat gcggagccag ggctccgcat ctcgcccttc
     3301 ttgattctct atggttgcgg cttctgcgga tgctcctcgt gatagagctt cgctgccgcg
     3361 tcaatctcat ccttgtggcg ctggatcacc tccaccatca cctgctggcc cagctcggcc
     3421 acggcatcct ggccttcatt cagaaccgcc ggcatcactg cgatcatgtg ccgccctgcc
     3481 ggagacttgt aaaacgcaag gatctgcgcc gcctcctcgg tggaaagccg ctgctgaaac
     3541 gagcgcacca tcgcgccttc aaaatctgcc gtgcccaggg tgcgctggag atcacccact
     3601 acatccgccg gcatccacgg cattatctcc ttcaggtggg gcagcagccc gtccagcatc
     3661 tcgcgctttt gcgtgttcgt ccccgtcagc tccagaatct cgtatacctg cgccgcggtt
     3721 accggatgcg cgggcctcac attctctgcc tgcgttgaag ctaccgcacg tgcgctcccc
     3781 ggcttccctg tattcgccgc caacagcggc attgatccca a
//
LOCUS       DASZYN010000657         8541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_229830, whole genome shotgun sequence.
ACCESSION   DASZYN010000657 DASZYN010000000
VERSION     DASZYN010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8541
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229830"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VHE33_16940"
     CDS             <1..745
                     /locus_tag="VHE33_16940"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HVT99195.1"
                     /translation="HHGGAWKVAYADFVTAMMALFIVLWLLNASKQVQEAVGGYFKDP
                     RGTAKVVGSNRDGSDNYVALKKEDMSKLKDQLLRSIHHIDQQDLLKDQIEITVTPEGL
                     RIELMESAKGTFFELGNATPTPALRELLAVLSHELAKLPNKVSIEGHTDSLVYSGLRN
                     YDNWDLSTDRANAARRLMETEGMESEQISQVRGFADRQPRLAQHPEDPSNRRVTLIVQ
                     YVVKDDSEVPLPAAVTTQNGVSTTPASPK"
     gene            complement(754..1101)
                     /locus_tag="VHE33_16945"
     CDS             complement(754..1101)
                     /locus_tag="VHE33_16945"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HVT99196.1"
                     /translation="MHKFTYRSPRFAVDFPVQLALDEGVIAGRCKEISTDGMQLELRR
                     PLPADFYGLVCLSWQNVHLELQVRLAHTGTKQEALRFVFASEKERAAVADLVARLADP
                     AEPADQPGLVLVR"
     gene            1391..2209
                     /locus_tag="VHE33_16950"
     CDS             1391..2209
                     /locus_tag="VHE33_16950"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HVT99197.1"
                     /translation="MISVINNISSLVAENALSSTQANLQNSLTQLATGLRLNHGSQDP
                     AAISISDGLGANIAALTQSSQNASNGVGLLQTADGALSQVTNLLNQAVTVATEAANGG
                     LTTAQATAANQEFQSILSEINQIGTTTNFNGVAVFSATAQTTFTSDGTASGATTTSTT
                     TGALSSTTLGLNADSLTSTTGAATALTDITTAIATIAATRGSIGASVNQLSADTNVEN
                     TEVSNLTSAQNNVRNADIGATVASMTQYNVLQQTGMAALSQSNSMEQNVLKLLQ"
     gene            2362..3780
                     /gene="fliD"
                     /locus_tag="VHE33_16955"
     CDS             2362..3780
                     /gene="fliD"
                     /locus_tag="VHE33_16955"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HVT99198.1"
                     /translation="MSSISALNALLSGSSSSSSAVNLTDILQAAFGASSPGIDVSSAV
                     NAAITAARSPENTWLSQENTIQSQISTLSALQNGVSALDNDLLSLNNLIGPLSANTVA
                     SSNSSVVTGSAVSGTAAGHHIVVVNSLARTASWASDTVASASTPLAAGSFTITGSDGT
                     ATTISTGDGTGTLSDIASTINADNLGLSASVVTDATGARLAIVSKTSGSAANFSVSDG
                     GGLTFGQIVNGSNASFSVDGLSLSSASNTVTGAVAGTTFNLLSASPGTEVSLTVSPDT
                     SQAATAINQFVTDYNSIISGLNSQFHDSGSGQGVLATDPTVRNLQNQLLGAMAYTYTP
                     ASGTTNVPNLSSMGITVNKDGTLSVDNAALNNTLQNDYTDVQNFFQGTAFNGFAGALD
                     QQLSDFLSPSDGAFTVDLQSLNSQYSTLQDDVTNFESNVITPLKSQLQAKYSQAEILL
                     QQLPMELKQIDQELGFNNNNNG"
     gene            3793..4257
                     /locus_tag="VHE33_16960"
     CDS             3793..4257
                     /locus_tag="VHE33_16960"
                     /inference="COORDINATES: protein motif:HMM:NF014604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HVT99199.1"
                     /translation="MTPTALYYRKTAAAASGGGLGVLIGLYDTLAGDLRRMAEAERGD
                     DLVRRCREANHALLVIGCLEDCVNRGTSGELADQLTAFYASLRTNLVLAQAKRSPEMI
                     EEQMAQVLKLREAWQAIELRPPAPEQEAPSTAQRFAAAAPEQPEPSRLGWTA"
     gene            complement(4358..5260)
                     /gene="flgL"
                     /locus_tag="VHE33_16965"
     CDS             complement(4358..5260)
                     /gene="flgL"
                     /locus_tag="VHE33_16965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HVT99200.1"
                     /translation="MRVNSNLIPNILADLQHSQAAVNTALEEVSTGKRVTQPSDDPGA
                     SAAMVQNGIDAANNDQYTQNITTLQTMAQAASSALSSVVSSLTQAVGLGTEGANGTNS
                     ASNLQALASQVQGILNSVVSQANTYVNGTYLFGGTVTGTVPYAADPASPNGYTYSGNN
                     NANSVAIGDHLNIQVSLPGSQVFSNPGNDVIGALSGLVTALKSGNSASIATATASVTS
                     AVNYMGQQEGFYSNVQSQLNAQGTALQQDKVTLTTQATSLVGVDLATAATDLTQAETA
                     NSAALAAAAKVLPVSLLNYLSPPQ"
     gene            complement(5275..6642)
                     /gene="flgK"
                     /locus_tag="VHE33_16970"
     CDS             complement(5275..6642)
                     /gene="flgK"
                     /locus_tag="VHE33_16970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HVT99201.1"
                     /translation="MSGLNTSLSIGVQSLEASEAALNATSNNIANANTPGYTREVAQF
                     SENQENLIGGVVSGGGVSLDSIQSVRDQLLNLQIQQQNSLQGSADTQSSILQQIQNNF
                     SSTGSDIGSALSAFSSSLAQLSANPSNGAVRQSVLSAGQNLANSFNNTAGGLMTAQSG
                     ADSQVTQTVAQINSITAKIAQLNAQLSPSVMAQQNGGTVQDQRDELVQQLSQLVGVSI
                     SQSSDGEVITTANGSPLVMGSHSFQMQTTKGTNGLQQVLDSNGNNITDAIQGGQLGGA
                     IQTRDQIIPGFMNSLDTLASQFATAFNAAQAAGFDSSGNPGQAFFSIPSGTTGAAAGI
                     KVALTSGSQIAVSSDGTAGSNGNVANLSAALTNALPSGLSASGAYSTLVYDVGNAASN
                     ANAQSSAVSQSLLQLTNQQGAVSGVNIDEETTNLIRYQTSYEAAARIVSTIEALTTAT
                     INMVS"
     gene            complement(6648..6980)
                     /locus_tag="VHE33_16975"
     CDS             complement(6648..6980)
                     /locus_tag="VHE33_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99202.1"
                     /translation="MTAIANNNLTELEESIAAQQDLSAQLGQLANQRRDTGTQSPPPH
                     HLDVELRSEIQTAAGELQNLNRRYALLIEHSSRSAAQMAALFRSFRGQFQEASGARGN
                     HATLSCQV"
     gene            complement(7128..7406)
                     /gene="flgM"
                     /locus_tag="VHE33_16980"
     CDS             complement(7128..7406)
                     /gene="flgM"
                     /locus_tag="VHE33_16980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HVT99203.1"
                     /translation="MRIDLLNSVASQIASEQSSQAAESQNASVAPREEDRATLVSDSG
                     SVGSLVSAAMSSPEIREDKVAALQQAISSGKYELDPAKIAASMIDEHA"
     gene            complement(7470..7880)
                     /locus_tag="VHE33_16985"
     CDS             complement(7470..7880)
                     /locus_tag="VHE33_16985"
                     /inference="COORDINATES: protein motif:HMM:NF021638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HVT99204.1"
                     /translation="MSAGMTGVTALTAQTSLQQNREARLLQEAHQLQSEQGSATDAKI
                     AKGAKDFEAMLLSSWLQQAEQSFATIPGAEDDDDAASRDQMMSFGTQALAQAMANSGG
                     IGIAKMIAEAMHRTAAKAEVTATALAEGKIDEKK"
     gene            complement(7881..>8541)
                     /locus_tag="VHE33_16990"
     CDS             complement(7881..>8541)
                     /locus_tag="VHE33_16990"
                     /inference="COORDINATES: protein motif:HMM:NF014206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HVT99205.1"
                     /translation="NDEFHKPVASALDNTRVDVNVASAGMGSVPELISRVQNLSLLVH
                     TPARIIINERTGTIVLGGDVKLTPVSVIHGNLSIEVSTTYTVAPVPGAYPPGWYGPGM
                     PPPGWGGPEGQPAAPSGTAQPQFQDQQDSRSRRNPGQDGPGNGSDARGAVGPTAALVP
                     ETTVSVNDQPAQTMRLDDGANVQELVNGLHALGTSSRDVVAILQAIKQEGGIQAEVEV
                     Q"
BASE COUNT         1601 a         2704 c         2584 g         1652 t
ORIGIN      
        1 ccatcacggc ggcgcctgga aggtggccta cgccgacttc gtcaccgcca tgatggcgct
       61 gttcatcgtg ctctggctgt tgaacgccag caagcaggtg caggaagccg tcggcggata
      121 cttcaaggat ccgcgcggca ccgccaaggt cgtcggcagc aatcgtgacg gctcagacaa
      181 ttatgttgcg ctcaaaaaag aggacatgtc gaagctgaag gatcagctcc tgcggtcgat
      241 tcaccacatc gatcagcagg acctgctgaa agaccagatc gaaatcaccg tcactcctga
      301 gggtctgcgg attgagctga tggagtccgc taaaggcacc ttcttcgaac tgggcaatgc
      361 cactccaacg cctgcgctgc gggaactcct ggccgtgctc tcccacgagt tggcgaagct
      421 gcccaacaag gtttcgatcg agggccacac ggattccctc gtctactccg gccttcgcaa
      481 ttacgacaac tgggatctct cgaccgaccg cgccaatgcc gcacgccgcc tcatggaaac
      541 cgagggcatg gagtcagagc agatatccca ggtacggggc tttgccgatc gtcaaccccg
      601 tttggcgcag catccggaag acccgtcgaa tcgcagggtg actctgatcg ttcagtatgt
      661 ggtgaaggat gattccgaag tgccgctgcc cgcagcggtc accacgcaga acggtgtctc
      721 tacaacaccg gccagtccaa agtgagcacg ggtctaacgg accagcacca ggcccggctg
      781 atcggccggc tctgcaggat ccgcaagccg cgccaccagg tcggctaccg ccgcgcgctc
      841 cttctccgaa gcaaagacaa agcgcagcgc ctcctgtttc gtcccggtat gcgccagccg
      901 cacctgcaac tccaggtgaa cgttttgcca cgacaggcaa accaggccgt aaaaatccgc
      961 cggcagaggc cggcgcagtt ccagctgcat tccgtcggtg ctgatctctt tgcaccggcc
     1021 tgcgatcacc ccttcgtcca gcgccagctg gaccggaaaa tcgacggcaa agcgcggact
     1081 tcgataggtg aacttatgca taggggatcg atctgcgaac gcagtatagc cgacgccgca
     1141 tgcgtcctcc tgctccccaa aggtgttacg gcaaccgcgc gtatcccccg tttgggggaa
     1201 tatgtagcag cgtccgtaac aatatacggg cgcgccgctc gcaaaaaagt ttgcactcgc
     1261 tctggaatct cctcagccag gggccgatag agttcctgca acaaccacct gctccatgtg
     1321 acgattccgg acctctaaga cggaagcggt tcggcgcagg cggaatccaa acaaggagcg
     1381 acacaccaac atgatcagcg taattaacaa catctcttcg ctcgttgcgg agaacgcact
     1441 gtcgtcaacg caggcgaatc tgcagaactc cctcacccag cttgctaccg gactgcgcct
     1501 gaaccacggc tcgcaggatc cggccgcaat ttccatctcc gacggcctcg gcgccaacat
     1561 tgcggctctg acgcagtcca gccagaacgc ctccaacggc gtgggccttc tgcagacggc
     1621 agacggcgcg ctctcccagg tgaccaacct gctgaaccag gcggtgacgg tcgcaacgga
     1681 agctgcaaac ggcgggctga ccacggcgca ggcgactgcg gcgaaccagg aattccagtc
     1741 gattctgtcg gaaatcaacc agatcggcac aaccaccaac ttcaacggcg ttgccgtgtt
     1801 cagcgcgacc gcccagacca cgttcaccag cgatggcacc gcgagcggcg ccaccacaac
     1861 ctccaccacg accggcgccc tgtcctccac gaccctcggc ctgaacgccg attcgctgac
     1921 ctcgacgacc ggagcggcga cagctctgac cgacatcacc accgccatcg cgacgattgc
     1981 agcgacccgc ggctccatcg gcgcctcggt caaccagctg tccgccgaca ccaacgtcga
     2041 gaacacggaa gtctccaacc tgaccagcgc gcagaacaac gttcgcaacg ccgacatcgg
     2101 cgccacggtc gccagcatga cgcagtacaa cgtactgcag cagaccggca tggcggctct
     2161 gtcccagtcc aactcgatgg aacagaacgt cctgaagctc ctccagtaat caacccgttg
     2221 tttccaccct cctcggcggt ggcatctctc gggggatgcc atcgcccttt tttggttccg
     2281 gcggctgaaa ttcgcactcc agagtgccga taacatcctg cgcgctttcc cagaccggag
     2341 gcgccgcaaa ggaaagtcat catgagttcc atctcggctc tcaacgcgct tctttcgggt
     2401 tcctcgtcct cgagctcggc cgtcaacctc acggatatcc tgcaggctgc cttcggcgca
     2461 tcctctcccg gaatcgacgt gagctccgcc gtcaacgcgg ctatcactgc cgcgaggtcg
     2521 cccgaaaata cgtggctgtc ccaggaaaac accatccaga gccagatcag cactctcagt
     2581 gccctgcaga acggcgtttc cgcgctcgac aacgatctgc tctcgctcaa caatctcatc
     2641 ggaccgctgt cggcgaatac ggtcgcctcc tcgaattcca gcgtcgtcac cggttcggct
     2701 gtctccggca ccgcggccgg ccaccacatc gtggtcgtca acagcctcgc ccgcaccgca
     2761 tcctgggcct cggataccgt ggccagcgcc agcacgcccc tggcggccgg cagttttacc
     2821 atcaccggat ccgacggtac ggccaccacg atctctaccg gagacggcac cggcacgctg
     2881 tccgacatcg ccagcacgat caatgccgat aacctcggcc tctctgccag cgtggtaacg
     2941 gacgcaactg gcgcgcgtct ggcgattgtc agcaaaacct cgggcagcgc agccaacttc
     3001 tcagtgagtg acggcggcgg ccttactttt gggcagatcg tcaacggcag caacgcttcc
     3061 ttctcagtcg acgggctcag cttgtccagc gcgagcaata cggttacggg cgccgttgcc
     3121 ggtacgacct tcaatctgct cagcgcgagt cctggaaccg aggtcagcct caccgtttcg
     3181 cccgacacca gccaggcggc cacggcgatc aatcaattcg ttaccgacta caactcgatc
     3241 atctcgggcc tgaattcgca gtttcacgac agtggctcgg gccagggcgt acttgccacg
     3301 gatccgacgg tccggaacct gcagaaccag ctcctgggag cgatggcgta tacctacacg
     3361 cccgcttcag gcacgacaaa cgtgcccaac ctttcctcca tggggatcac cgtcaacaag
     3421 gacggcacac tcagcgtcga caacgcggcc ctcaacaaca cgctgcagaa cgattacacc
     3481 gacgtgcaga atttcttcca gggcacagcc ttcaacggct ttgccggtgc actcgaccag
     3541 cagctcagcg acttcctcag cccgtcggac ggggctttca cggtcgatct gcagagcctc
     3601 aacagccagt acagcaccct gcaggacgac gtcacgaact tcgaaagcaa cgtgatcacg
     3661 ccgctgaaga gccagctgca ggccaagtac agtcaggccg aaatcctgct gcagcaactg
     3721 ccgatggaac tgaagcagat cgaccaggaa ctcggcttca acaacaacaa caacggataa
     3781 cgcgggacca ccatgacgcc aacagcactc tactatcgga agacggccgc cgccgccagc
     3841 ggcggcgggc tcggggtcct catcggcctt tacgacacac tcgccgggga tttgcggcgg
     3901 atggcggagg cagaacgcgg cgacgacctt gtgcgccgct gccgcgaagc caatcatgcg
     3961 ctgctggtga tcgggtgcct tgaagactgc gtcaaccgcg gcaccagtgg cgagctggcc
     4021 gatcagctca cagcgttcta tgcgtcgctg cgcacgaacc tggttctcgc ccaggcaaag
     4081 cgctcgcccg agatgatcga agagcagatg gcgcaggtgc ttaagctgcg ggaagcgtgg
     4141 caggcgatcg agttgcgtcc gccggcgccg gaacaagaag ccccctcgac tgcgcaacgg
     4201 ttcgcggctg cggccccgga gcagccggag cccagtcgtc ttggctggac cgcataactc
     4261 ctcaacaact ggaaatagcc gaagacgccg caacgggcga atggccgctt gagcgcggct
     4321 tagcctaacg tcatcgatcg ctcgggctgc gggtgcttta ctggggtggg ctgaggtagt
     4381 tgagcaagga caccggcagc accttggctg cggctgccag cgcagcgctg ttcgccgtct
     4441 ccgcctgggt gagatccgtg gcggctgtcg ccagatccac accgacgaga ctggtcgcct
     4501 gcgttgtgag ggtcaccttg tcctgctgca gggcggtccc ctgggcgttc aactggctct
     4561 ggacgttgct gtaaaagcct tcctgctgcc ccatgtagtt cactgcggag gtaactgagg
     4621 ccgtggccgt cgcaatgctg gccgagtttc cgctcttgag ggcggttacc aaaccactca
     4681 gcgcgccgat gacgtcgttg cccgggttgg agaagacctg gctcccgggc aggctcacct
     4741 ggatgttcag gtggtcgccg atcgcgaccg agttcgcatt gttgttcccg ctgtacgtgt
     4801 acccgttggg agaggcggga tcggccgcgt acggaacggt accggtgacc gtacctccaa
     4861 acaaatacgt gccgttgacg taggtgttgg cctgggagac gacgctgttg aggatgccct
     4921 gcacctggct ggccagcgcc tgcaggttgc tggcagagtt ggttccattg gcgccttcgg
     4981 tgccgagccc aacggcctgt gtcagcgagg agaccacgct gctcaaggcg gaactggccg
     5041 cctgcgccat ggtttgcaga gtcgtgatgt tctgtgtgta ctggtcgtta tttgccgcgt
     5101 cgatgccgtt ctgcaccatt gccgcggagg cgccaggatc gtcagaaggc tgggtcacgc
     5161 gcttgccggt ggagacttcc tcaagcgcag tattgaccgc cgcctggctg tgctgaagat
     5221 ccgcgaggat attgggaatg agattggagt tgacacgcat agggtccgcc ctggctagct
     5281 caccatgttg atggtggcgg tggtgagtgc ctcaatggtg ctcacgatgc gagctgccgc
     5341 ttcgtaagag gtctgataac gaatcagatt ggtcgtttct tcgtcaatgt tgacgccgga
     5401 aaccgctccc tgctgattgg tgagctgtaa caggctttga ctgacggcgc tggactgggc
     5461 gtttgcgttg gaagccgcat tgcccacgtc atacaccagg gtcgagtaag caccggatgc
     5521 ggatagtccg gacgggagcg cattcgtaag cgccgccgaa aggttcgcga cgttgccatt
     5581 gctgccggca gtgccatcgg aactcacagc aatctgcgag ccgctggtca gcgcaacttt
     5641 aatgcctgcc gccgcgcctg tggttccgga gggaatgctg aagaaggcct gaccggggtt
     5701 gccgctggaa tcgaaacctg ccgcctgggc ggcgttgaac gccgtagcga actggctggc
     5761 cagggtgtcg agcgaattca tgaagcccgg aataatctgg tcgcgcgtct gaatcgcgcc
     5821 gcccagctgg ccgccctgga tcgcatccgt gatgttgttg ccgttcgagt ccagcacctg
     5881 ctgcaaaccg ttggtgccct tggtcgtctg catctggaag gagtggctgc ccatgaccag
     5941 aggcgatcca ttcgcggtcg tgatcacttc gccgtcgctg ctctgcgaga tggaaacgcc
     6001 gaccagctgc gacaactgct gcaccagctc atcgcgctga tcctgaaccg tgccgccatt
     6061 ctgctgcgcc atgaccgaag gcgagagctg cgcgttcagc tgggcgatct tcgcggtgat
     6121 ggagttaatc tgcgctaccg tctgggtgac ttgagaatca gcgccgcttt gagccgtcat
     6181 cagaccgccc gcggtgttgt tgaaggagtt cgccaaattc tggcctgcgg agagcacgct
     6241 ctgccgcacc gctccattcg atggattggc ggacagctga gccaggctcg acgagaaggc
     6301 actcagggcg ctgccaatat cgctgccggt gctcgagaag ttgttctgga tctgctgcag
     6361 gatcgaagac tgcgtatcgg cgctgccctg cagtgagttc tgctgctgga tctgcaggtt
     6421 gagcagttga tcgcgtacgc tctgaatgct gtcgagcgag acaccgccgc cgctcacgac
     6481 gccgccgatc aggttctcct gattctcgct gaactgcgcg acctcgcgcg tatagccggg
     6541 cgtgttggcg ttggcgatgt tgttgctcgt ggcgttcaga gccgcttccg aggcttcgag
     6601 cgactgcacg ccgatcgaca acgatgtgtt gagtccagac atggcgatca gacctggcag
     6661 gacagggttg cgtgatttcc cctggcgcca gaagcctcct gaaattgtcc cctgaaggaa
     6721 cgaaacaaag ccgccatctg cgcggccgaa cggcttgaat gctcgatgag aagggcgtag
     6781 cgccggttca ggttctgcaa ttcgccggca gcggtttgaa tctcgctgcg gagctccacg
     6841 tcgagatggt ggggcggagg cgattgtgtg ccggtgtcgc ggcgctggtt cgccagttgg
     6901 ccaagttgag cgctgagatc ctgctgggcg gcgatgctct cttcgagttc agtgagattg
     6961 ttgttggcaa tagccgtcat cgcgcgttcc agttcgcgcg caagcgagcg caggagtgca
     7021 agatgctctc cggcttgatg actccgagtt tgggcgtcag cccctgtcat cctggcactt
     7081 tcccggtcac gtcattgccg cgcgacggct gaggctctcg cggcggtcta ggcgtgctcg
     7141 tcgatcatgg acgcggcgat ctttgccggg tcgagttcat actttccgct gctgatagcc
     7201 tgctgcaaag ccgcgacctt gtcctcgcgg atctcgggcg aactcatggc ggcgctcacc
     7261 agcgaaccga cggatccaga gtcggagacc agggtcgcgc gatcttcctc gcgcggcgcg
     7321 accgaggcat tctgcgactc cgctgcctgc gagctctgct cactggcgat ctggctggcg
     7381 accgaattca gaagatcgat tctcatagct gtttccctct tgtaggctgc gttactatcg
     7441 gcacttctcg ccggagttta aggtaaaact cactttttct cgtcaatttt tccctccgcc
     7501 agagccgtag ccgttacctc agccttcgcc gccgtccggt gcatggcttc ggcgatcatt
     7561 ttcgcgatgc cgatgccacc ggaattggcc atcgcctgcg ccagggcctg ggttccgaag
     7621 ctcatcatct gatcgcggct ggccgcatcg tcatcgtctt cagcgccggg gatggtggca
     7681 aaggactgct cagcctgctg cagccagctg ctcaggagca tggcctcgaa gtcttttgct
     7741 ccctttgcga tctttgcgtc ggtggcgctt ccctgttcgg attggagctg atgcgcctcc
     7801 tggagcaggc gtgcctcgcg gttctgctgc aggctggttt gcgcggttaa agcggtgact
     7861 ccagtcatac cggcgctcat ttactgcacc tccacttcgg cctgaatgcc gccttcctgc
     7921 ttgatggcct gcagaatggc gacgacgtcg cgcgaggagg ttccgagcgc gtggagtccg
     7981 ttgacgagtt cctgaacgtt ggcgccgtcg tcgaggcgca tggtttgagc cggctgatcg
     8041 ttgacgctga cggtggtttc cggaaccagc gcggctgttg gaccgacggc accccgagca
     8101 tcggatccat tgcccggtcc gtcctgccca gggttgcggc ggctccgact gtcctgctgg
     8161 tcctggaact ggggttgtgc ggtaccggat ggcgcagccg gctgaccttc gggtccgccc
     8221 cagcccggag ggggcattcc cggcccgtac cagcctggcg ggtacgcgcc gggcaccggt
     8281 gcaactgtgt acgtggtgga aacttcgatg gataaattgc cgtggatgac tgataccggg
     8341 gtcaacttga cgtcaccgcc gaggacgatg gttccggttc tctcattaat aatgatgcga
     8401 gccggcgtgt ggaccaggag cgagaggttc tggacgcgcg agatcagctc gggaaccgag
     8461 cccattccgg cactggcgac attgacgtcg acgcgggtgt tatcgagtgc ggaggctacc
     8521 ggcttgtgga actcgtcgtt g
//
LOCUS       DASZYN010000658         4020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_941617, whole genome shotgun sequence.
ACCESSION   DASZYN010000658 DASZYN010000000
VERSION     DASZYN010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941617"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VHE33_16995"
     CDS             complement(<1..420)
                     /locus_tag="VHE33_16995"
                     /inference="COORDINATES: protein motif:HMM:NF037701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99206.1"
                     /translation="MPPHRPSLSLLLFAFLLTGAHASPTLHKASTLPNLKPLPLVSGG
                     RMRITPSPSPFGANDYTSQWPGSYFRAAFIGPTVYFRVGTSHEILHVLVDGQPLPPLV
                     QPEPGPWQVEGLSNARHEIGLFVATESQDSPNTFGGFA"
     gene            676..1446
                     /locus_tag="VHE33_17000"
     CDS             676..1446
                     /locus_tag="VHE33_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99207.1"
                     /translation="MKGALQVLLIVGLAVGTVWAAENPFAGRWKMDPSKSTMVDEMKV
                     ASAGGNQYSFDFGGGTGEKIKVDGTDQPGMFGTTLAVTAEAPDRWKVVRKKDGRTMLI
                     GKWTLSADGRTLTDDYTELDAKGSPATHLLYKYERMGGGLGFSGDWVSSSQPTDTVVV
                     EIAVWEGDGLALTRRSVTRHVKFDGKAYPAEGANLPAGYGTSGRRVDARTVELTDKIG
                     DKARDSQTMTVSEDGKTLTMRMTPVGRARPSVMVFERE"
     gene            1582..2328
                     /locus_tag="VHE33_17005"
     CDS             1582..2328
                     /locus_tag="VHE33_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99208.1"
                     /translation="MAGLRGRTRTRGVVATALAAAMLVVAWANFRPRVFGQAAKAPPA
                     DAKPEEPVGEYSLVTTVLRPSFSTHLLNPPIAIAVDGGTDRAPGEISATKPIPAEFAI
                     EGFGIPRYECASTNPKLRGCEALDRTDLAIRDANTLGYHIVSHGAAVTLNVNLEVHDM
                     LPVSVSTPNAPWHAREMIFVTLPKATPAVHFVSEVLVGEWNGEPIVFEVGKALPDSAK
                     KGLEDLGVKQDLGEGVLYSFRVKEPPKKQE"
     gene            2582..2977
                     /locus_tag="VHE33_17010"
     CDS             2582..2977
                     /locus_tag="VHE33_17010"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVT99209.1"
                     /translation="MEEQGSQRRARRSSAGHAEKPPAVAGVRLHPERSGQWMEASALR
                     QWALEQLNCDEPARELVIDLEGLEHLDASALQVLLALRAEQVRRGGVLRFEHASPGLR
                     PWFVWAGAAECAGFAEPGKQAAAEKGEDA"
     gene            2970..3332
                     /locus_tag="VHE33_17015"
     CDS             2970..3332
                     /locus_tag="VHE33_17015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT99210.1"
                     /translation="MRKVLIVDDSVSMRQMVSFTLRQGGFDVVEAEHGQAAIDKLKGT
                     SVDLVITDLNMPVMDGITLIRKLRADAGTKSKPILMLTTEGLAAKKEEGKAAGATGWI
                     VKPFDPEKLLATLARVLP"
     gene            3337..>4020
                     /locus_tag="VHE33_17020"
     CDS             3337..>4020
                     /locus_tag="VHE33_17020"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HVT99211.1"
                     /translation="MKIDLTQFRQTFLQESADHLATMESGLLALRTHPEDDETLHAVF
                     RSAHSIKGGAGSVGLEALARFTHGMENLLDRLRAHEMAISEAIIAELLAAVDALHTLL
                     GAEPGAELPEDAQRLAERIEALATGESQAAEPVAAAAAAEANHGEMAAYRVTFRPDRE
                     MFQSGTNPIVLLRNLASLGDVSVTHLDTGDLPALAELDPEQCYLSWTVEMATSRGEQE
                     LREVFEFVEH"
BASE COUNT          747 a         1056 c         1550 g          667 t
ORIGIN      
        1 ggcaaagccg ccgaaggtgt tgggagaatc ctggctctcg gtggcgacaa agaggccgat
       61 ttcgtggcgg gcgttgctga ggccttcgac ctgccacggt ccgggctcgg gctggacgag
      121 gggcgggagg ggctggccat cgacgaggac gtggaggatc tcgtgactcg tgccaacgcg
      181 gaagtagacg gtaggaccga tgaaggcggc gcggaaatag ctgccgggcc actgggaggt
      241 gtagtcgttg gcgccgaagg gggatggaga gggcgtgatg cgcatgcggc cgccgctgac
      301 gagggggagg ggtttgagat tgggaagggt cgaggccttg tggagggtcg gtgatgcgtg
      361 agcacccgtg aggaggaagg caaacagcag cagcgagagg gaggggcggt gagggggcat
      421 cgcagtcgaa tctatcagga cggacgtgtc cggtggcaga acagtggcgt ccgtgggtgg
      481 acagtggggg cacggcggat acctgcaatc tggtgcgggt cgctggcgtc cgaaagacgg
      541 gaatcaagca ggatgggcac tcaggccagg ttctccaggc ctggcgtgcg tgggtgcagg
      601 actggcctct ggtgtgctgt cgaggcaggc gagtccgggc tcggcgggca ttcctgtgtt
      661 tgggaggagt tgacgatgaa gggtgcgttg caggtgttgc tgattgtggg tctggcagtg
      721 gggacggtgt gggcggcgga aaatccgttt gcgggccgat ggaagatgga cccgtcgaag
      781 agcacgatgg tggacgagat gaaagtcgct agcgccggcg gaaatcagta ttcgttcgat
      841 tttggcggcg ggaccggcga gaagatcaag gtggatggta cggatcagcc ggggatgttc
      901 ggcacgacac tggcagtgac ggcggaggcg ccggatcgct ggaaggtggt gcgaaagaag
      961 gacgggcgca cgatgctgat cgggaaatgg acgctctcag cagacggcag gacgctcacc
     1021 gacgattaca ccgaactcga tgcaaaggga tcaccggcca cgcatctgct ctataagtac
     1081 gagcggatgg gaggcggctt ggggttcagc ggggactggg tgagcagcag ccagccgacc
     1141 gacaccgtgg tggtggagat tgcggtgtgg gaaggcgacg ggcttgccct gacgcgacgc
     1201 agcgtgacga ggcacgtgaa gttcgacgga aaggcttatc cggccgaggg cgcgaatctg
     1261 ccggcaggct atgggacttc ggggcggcga gtggatgcgc ggacggtgga gttgacggac
     1321 aagattgggg acaaagcacg cgacagccag acgatgaccg tatccgaaga cggaaagacg
     1381 ctgacgatga ggatgacgcc ggtgggccgg gcgcggccaa gcgtgatggt gttcgagcgg
     1441 gagtgaggga acgccggggc gggttgaggg aggttcgttt ggttccgcgt tggaacaccg
     1501 gttccgttcc gggaatcggc gcgagttcgg gggctggatt cgcggctccg ccgcgtcgtt
     1561 gcgcgctatg atccagtcgg catggcagga ttgcggggac gaacacgcac acgcggcgtc
     1621 gtggcgacgg cactggcagc cgcgatgctg gttgtggcct gggcgaactt ccggcctcgt
     1681 gttttcgggc aggctgcgaa ggctcctccg gccgatgcca aaccggagga accggtcggt
     1741 gaatactcgc tggtgacgac ggtcctgcgt ccctccttca gcacgcatct gctcaatccg
     1801 ccgatcgcga ttgccgtgga cggcggaacg gatcgcgcgc cgggcgagat ttccgcgacg
     1861 aagccgatcc cggcggagtt cgcgattgag ggcttcggga ttccccggta cgaatgcgcc
     1921 tccaccaacc caaaactgcg cgggtgcgag gcgctggacc gcacggatct tgccattcgc
     1981 gacgcgaaca cgctgggcta tcacatcgtg agtcacgggg cggcggtgac gctcaacgtg
     2041 aatcttgagg tgcacgatat gctgccggtt tcggtgagca cgccgaacgc gccgtggcat
     2101 gcgcgggaga tgatctttgt gactttgccg aaggctacgc cggcggtgca ctttgtgtcg
     2161 gaagtgctgg ttggggagtg gaacggggag ccgatcgtct ttgaggtggg caaggctctg
     2221 ccggattcgg cgaagaaggg tctggaggat ctcggcgtga agcaggatct cggcgagggg
     2281 gtgctgtaca gcttccgggt gaaggagcct ccgaaaaagc aggaatgaaa gcgtgtggcg
     2341 gatagcggat agcgctttcg aacagcgagg gaaaagaagg gatttctgag gggcagcgtg
     2401 tcccccggcg tcctggggac cggacggtcc ggcgcaggca catcggcgga ctgtcccact
     2461 ttgggcacgc gttcatgcgt ccccctccac cgccgataca actctttgcg ggaagcaaca
     2521 ctttctttgc agcggcctgg aggtcctgtg gccgcgagtg gacccgtcga gaggaacaag
     2581 gatggaagaa caggggagcc agcggcgcgc tcgacgcagc agcgcaggtc atgcggagaa
     2641 acctccggcg gtggccgggg ttcgactgca tccggaacgg tccggccagt ggatggaggc
     2701 gtcggcattg cggcagtggg cgctggagca attgaactgt gacgagccag cgcgggagct
     2761 ggtgatcgat ctggaagggc tcgagcatct ggacgcgagc gctctgcagg ttttgctggc
     2821 tcttcgcgcg gaacaggttc gccgcggcgg ggtcctgcgg ttcgaacacg cttcccccgg
     2881 cttgcggcca tggtttgttt gggcgggagc cgcggagtgc gcggggttcg ccgagccggg
     2941 aaaacaagcc gcggcggaga agggcgaaga tgcgtaaggt tctgatcgtc gacgattcag
     3001 tttcgatgcg gcagatggtg agcttcaccc tgcgccaggg cggtttcgac gtcgtcgaag
     3061 ccgagcatgg gcaggcagcg atcgacaagc tgaagggcac ctccgtcgat ctggtgatca
     3121 cggacctgaa tatgccggtg atggacggca tcacgctgat ccggaagctg cgcgccgacg
     3181 cgggcacgaa atcgaaaccc attctgatgc tgaccaccga agggctggca gcaaaaaagg
     3241 aagaaggcaa ggccgcgggt gccaccggct ggatcgtgaa gccgttcgat ccggagaagc
     3301 ttttggcgac cctcgcgcgg gtcctgccgt gacgccatga agattgacct gacccagttt
     3361 cgacagacat ttctgcagga gagcgccgat cacctggcca cgatggagtc ggggctgctg
     3421 gcgctgcgca cccatccgga agacgatgag acgctgcatg cggtcttccg ctccgcgcat
     3481 tcgatcaaag gcggcgcagg ctcggtaggt ctggaagcgc tggcccggtt cacgcatgga
     3541 atggagaacc tgctcgaccg cctgcgcgcg cacgagatgg cgatcagcga ggcgatcatc
     3601 gccgaactgc tggcggcggt cgatgcgctg cacacgctgc tcggcgccga accgggcgcc
     3661 gaactgccgg aggacgcgca gcgtctggcc gagcgcatcg aggcgctggc cacgggcgag
     3721 agccaggctg cggaaccggt tgccgcggct gcggccgccg aagcgaatca cggggagatg
     3781 gcggcgtatc gggtcacctt ccggcccgac cgcgagatgt ttcagagcgg cacgaatccg
     3841 atcgtgctgc tgcgcaatct ggcttccctg ggagacgtca gcgtcacgca cctcgacacc
     3901 ggcgatctgc cggcgctggc cgaactcgac ccggagcagt gttatctgag ctggacggtg
     3961 gagatggcca ccagccgtgg ggagcaggag ttgcgcgagg tcttcgaatt cgtggagcac
//
LOCUS       DASZYN010000659         1666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1062374, whole genome shotgun sequence.
ACCESSION   DASZYN010000659 DASZYN010000000
VERSION     DASZYN010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062374"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(354..959)
                     /gene="ispF"
                     /locus_tag="VHE33_17025"
     CDS             complement(354..959)
                     /gene="ispF"
                     /locus_tag="VHE33_17025"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HVT99212.1"
                     /translation="MDIRIGYGWDSHAFKPGVPLKIGGLSIDHPEGLAGHSDGDVLLH
                     ALTDALLGAVSAGDIGSFFPPGDPRWKDADSKIFLELAKEEIRNAGYRIVNVDTTLVL
                     NAPKIGPISSEMRERVAELLEILPINVGIKAKTPEGLDVDHVAQAHAVVLLERIEDAG
                     EVQSMSAMIQSQRELEDVVRDLVSQVHGVAVKAPFDTDDIT"
     gene            complement(1024..>1666)
                     /gene="ispD"
                     /locus_tag="VHE33_17030"
     CDS             complement(1024..>1666)
                     /gene="ispD"
                     /locus_tag="VHE33_17030"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HVT99213.1"
                     /translation="QFQELAGKPILIRTLQAFAEVAEVTSLVVAVRATEMERLTAQIF
                     EHGFAERVRVVEGGDSRQESVCNALKTLDCAEDDIVLVHDAVRPLIEPAVIRRVIEAV
                     QKHDAAIVGLPAVDTIKQVERTADGAIVTATIPREYIVQAQTPQGFRCGLLRRAFAEA
                     EADGFSGTDEASVVERAGSSVFVVPGSASNLKITQPGDLELAEFYLAHRSASH"
BASE COUNT          332 a          530 c          484 g          320 t
ORIGIN      
        1 ctcttgtgcc agaagaaaac aacccggcaa agagcagaaa agcatggatc cgaccgccca
       61 acagcccgcg gcgacgcaga aacgaagagc gcgaagtgac actcgatcct ccgggaaatt
      121 cctgccttgc ggagccgatt cctgcagctc catcaacgcg aaaacaagtt tgtacgtcgg
      181 gggttggcca ggaaccgatg cgaaacgcag acagaggatt tcgccgcagt tcagtcgatg
      241 gcaacgctac catcggagtt cgggaaattg taccgctcgc gcttacgaaa tgggggatca
      301 ggcgatcagg agccgaagtt tcggactgga tgcagggctg caggattcag aggttaagtg
      361 atgtcgtcgg tgtcaaacgg cgcttttact gcaacaccgt gcacctggct gaccaggtcg
      421 cgcaccacat cttccagttc gcgctggctc tggatcatgg cgctcatgct ctggacctcg
      481 cccgcgtcct caatgcgctc cagcagcacc acagcgtggg cctgcgctac atggtccaca
      541 tccagtccct cgggcgtctt tgctttgata ccgacgttga tcggtaagat ctccaggagt
      601 tccgccacgc gttcgcgcat ctcgctcgaa atcggtccga tcttcggcgc attgagcacg
      661 agcgtggtat ccacgttcac gatccggtag ccggcgttgc ggatttcttc tttcgccagt
      721 tccagaaata tctttgagtc ggcgtctttc cagcgtggat ctcccggcgg aaagaagctg
      781 ccgatatcac ccgcggacac cgccccgagc agtgcgtcgg tgagggcgtg caggaggaca
      841 tcgccatccg aatggcccgc caggccctcg ggatgatcga tgctgagacc gccaatcttc
      901 agcggcacgc ccggcttgaa tgcgtgcgaa tcccagccat aaccaatgcg aatgtccatg
      961 cgcggttccc tgcaacctcc tcagggtaca tcagcctttt ccagtggctg gttactgtga
     1021 atgttaatgc gacgcgctac ggtgcgcgag ataaaactcg gccagttcca gatcgccggg
     1081 ttgcgtaatc ttcaggttcg acgccgaacc ggggacaaca aagactgagc ttcccgcgcg
     1141 ctccacgacg ctggcttcgt cggttccgct gaacccatcg gcttccgcct cggcaaaggc
     1201 ccggcgcagt aagccacagc gaaaaccctg gggcgtctgc gcctgcacga tgtactcgcg
     1261 cggaatggtg gcggtcacga tcgcgccgtc cgccgtccgc tccacctgct tgatggtgtc
     1321 gaccgcgggc aagcccacga tcgcggcgtc atgtttctgc acggcctcaa tcacgcgacg
     1381 aatcaccgcg ggctcaatca gcgggcgtac cgcatcgtgc accagaacga tgtcgtcctc
     1441 tgcgcagtcc agggtcttga gcgcattgca gacgctttcc tgccgggaat cgccgccttc
     1501 gacgacgcgg acccgctcgg caaatccatg ttcgaagatc tgcgccgtca gccgctccat
     1561 ctcagtcgcg cggacggcga ccaccagcga tgtcacctcc gcaacttcgg caaaggcctg
     1621 cagggtgcgg atgaggatcg gctttccggc cagctcctga aactgc
//
LOCUS       DASZYN010000660         3969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_941665, whole genome shotgun sequence.
ACCESSION   DASZYN010000660 DASZYN010000000
VERSION     DASZYN010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941665"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VHE33_17035"
     CDS             <1..913
                     /locus_tag="VHE33_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99214.1"
                     /translation="TVTGRQYPLLFQVGETAYGNPITDGQHPHDFFMEVAALYDVSIS
                     HNTLLSLYLAPVGDPAIGPIAYPHRASALENPVATLGHHQEDSTHISDDVITLGVTHG
                     WARAEASGFHGREPNEHRWQVQQGAIDSWSTRLTLQPGQNWSGQLSYGRLHSPEALFP
                     AEDQARTTASVMYNRPLRAGNWTSTLVWGHTRSLADNIVQNSYLLESALQFHTSNHVW
                     TRIEDDDRTTELLLGERAIPAGFEEKPAGRVQAYTIGYDRDLPLMAHASTALGAQFTA
                     DTVGSRLKPAYGSDPVGVVFFLRIRPN"
     gene            complement(986..1192)
                     /locus_tag="VHE33_17040"
     CDS             complement(986..1192)
                     /locus_tag="VHE33_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99215.1"
                     /translation="MEQQRSAEPARDGRPTAEQVAALATRERERQALELQRERILDER
                     TSNPIRRKALQAALDEIEAKLKAL"
     gene            1325..2338
                     /locus_tag="VHE33_17045"
     CDS             1325..2338
                     /locus_tag="VHE33_17045"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HVT99216.1"
                     /translation="MKAWRIPSFGIENLEQVTLPDPQPQPGEVVVRVHAVSWNYRDLR
                     MVQGLYNPKMPLPRIPCSDGAGEVIAVGDRVTRAKVGDRVCAIFMQNWIEGPPDAAKH
                     RGALGGDIDGMLAEQVVLPETGIVKFPEFLSYEKAATLPCAAVTAWNALTRAHRIKPG
                     DTVVILGTGGVSIFALQFAKTMGARVLGTSSSDAKLERAKTLGLDAGINYSEHPEWDR
                     WVVEQTGGRGADLVVEVGGAGTFNRSLRATRIGGAVAQIGVVSEGSDPVLIPLLFHRQ
                     IRLRGIYVGSRSDFEMMSRAIQQHKLEPVVDEVFSFDAAGEALLRMQSGAHFAKLVTS
                     VTH"
     gene            2421..2708
                     /locus_tag="VHE33_17050"
     CDS             2421..2708
                     /locus_tag="VHE33_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99217.1"
                     /translation="MNFLKTCLQAVLNFFSEILFGCTHTRVTRPFTLGSETYEVCLDC
                     GKQIYYSATEMRRLTPFEVRRLKAARAGEVKVVPINTPHLVPRDRKPNVAA"
     gene            2921..>3969
                     /locus_tag="VHE33_17055"
     CDS             2921..>3969
                     /locus_tag="VHE33_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99218.1"
                     /translation="MARSLPGRLLLASLALGVAGCGSINYAAQAGDGSFALRASATSV
                     ATNGQVALQAVLPSGESAPVAWQIASGTNAASLGQGHIDANGIYTAPGSLSADSIAVR
                     LEATLSNEAKSTASLAVTVHPGFVQPLLPQNVALTPGATAEVTAQIAEVDSGSVSWRL
                     AATPTGPENAALGTLSGSECRRSSGRYTSCSVLYTAPSALPAGSVYVVAAVNGTLTTA
                     PLRVLLNNQGIVSNPVTNQALQQGAILLGGSGGNDGDFDTYEDHDGAPFIADCCGGTL
                     GALVEDQSGDQYILSNNHVLAESDQGHIGDTIDQPGLIDGACTPLTRPGSTLRPVGTL
                     ESFVPLASKQTNVDAA"
BASE COUNT          751 a         1345 c         1092 g          781 t
ORIGIN      
        1 tacggtcacc gggcggcagt atccgctgct ctttcaagtc ggcgaaaccg catacggaaa
       61 cccgatcacc gacggccagc acccgcatga tttcttcatg gaggtcgcgg cgctttacga
      121 tgtctcaatc tcgcacaaca cattgctgag cctctatctg gcgccggtcg gcgaccccgc
      181 catcggaccg attgcctatc cgcatcgcgc atcggcgctg gagaatcctg tcgcgacgct
      241 gggccatcac caggaagact ccacgcacat ctccgacgat gtgatcacac tcggtgtcac
      301 ccacggctgg gcgcgcgcgg aggccagcgg cttccatggc cgcgagccca atgagcatcg
      361 ttggcaggtg cagcagggag cgatcgattc ctggtccaca cgcctcaccc tgcagccagg
      421 ccagaactgg agtggccagc tttcttatgg ccgcctgcat agccccgaag cgctctttcc
      481 cgccgaggac caggcgcgca ccaccgcgtc cgtcatgtac aaccgtccgc tcagggcagg
      541 gaactggacc agcactctcg tatggggaca cacgcgttct ctcgccgaca acatcgtcca
      601 gaacagctat ctgctcgaat cggcgctgca gttccacacc agcaaccatg tctggacgcg
      661 aatcgaagac gacgatcgca caacggaatt gcttctcggt gagcgcgcga tccccgcggg
      721 tttcgaggag aaacccgccg gacgcgtgca ggcgtatacc atcggctacg atcgcgatct
      781 gccgctcatg gcgcatgcga gtaccgcgct tggcgcacag ttcacggccg ataccgtggg
      841 ctcgcgcctg aagcccgcgt acggttcaga tcctgtgggt gtcgtatttt tcctgcgcat
      901 ccggccgaac tagcttcagc cgcaacttgc gcgcttttgg ctcgctgtgc gcgcgccgtt
      961 gactcgccgt tcgctcgctt tttctctaaa gcgctttcag cttcgcttcg atctcatcca
     1021 gcgccgcctg caaagctttg cgccggattg gattcgacgt gcgctcatcg aggatccgtt
     1081 cgcgctgcaa ttccagcgcc tgacgttcgc gttcgcgcgt agccagcgcg gcaacctgtt
     1141 cggctgtggg cctgccatcc ctcgccggtt ccgcagaccg ttgctgctcc acctgctctt
     1201 ccactgattt gctttcccat ccgcgcgcca tgcatacagg gtacagggga cacggtgcag
     1261 ggtacagagt accgatgacc tctggctgtc cgcttcgtgc tcagatagga agagaggtgc
     1321 attcatgaaa gcctggcgta tcccatcctt cggcatcgag aacctcgaac aagtcactct
     1381 gcctgacccg caaccgcagc ccggcgaagt cgtcgtgcgc gtccatgccg tctcgtggaa
     1441 ctaccgcgat ctgcgcatgg tgcaggggct ctacaacccg aagatgccgt tgccgcgtat
     1501 tccttgctcc gatggcgcgg gcgaagtcat tgccgtcggt gaccgcgtca cgcgcgccaa
     1561 agtgggcgat cgcgtctgtg ccatcttcat gcagaactgg atcgaaggcc cgcccgacgc
     1621 agcaaaacat cgcggcgcac tcggcggaga catcgacggc atgctcgccg aacaagtcgt
     1681 tcttcccgaa acgggaatcg taaagtttcc cgaatttcta tcgtatgaaa aagctgcaac
     1741 gctgccctgc gcagcagtca ccgcctggaa cgccctgacg cgtgcccaca ggatcaaacc
     1801 cggcgacacg gtggtcatcc tgggcaccgg aggagtgtcg atcttcgcac tccagttcgc
     1861 aaaaacgatg ggcgcacgcg tgctcggaac gtcgagcagc gacgccaaac ttgagcgcgc
     1921 gaagacactc ggtctcgatg ccggcattaa ctacagcgaa catccggaat gggatcgatg
     1981 ggtcgtggag cagaccggcg gccgcggggc tgacctcgtt gtcgaagtcg gcggcgccgg
     2041 cacattcaac cgttcgctgc gggccactcg gatcggcggc gcggtcgcgc aaatcggcgt
     2101 cgtgagcgaa ggttccgacc ccgtgctcat cccgttactg tttcacagac agattcgcct
     2161 ccgcgggatt tatgtaggtt ccagatccga ttttgagatg atgagtcgag ccatccagca
     2221 gcacaaattg gaacccgtgg tggatgaagt atttagcttc gacgcggctg gggaagcttt
     2281 gctccgcatg cagtctggag cgcatttcgc gaaattagtt acttccgtga cgcactagaa
     2341 agtttgtcca ccaggcgaca ttttgcctca aactgcatct gaagaagtgc ccgaaaacaa
     2401 catacgagag gagggctccc atgaacttcc tgaagacctg cctgcaagcg gttctcaatt
     2461 tcttttcaga gattcttttt ggatgcaccc acacgcgagt cacccgtccc ttcaccctcg
     2521 gcagcgaaac ctacgaggtg tgcctggatt gcggcaaaca aatctactac tcggcaacag
     2581 agatgcggcg tctcaccccc tttgaagtac gccgtctgaa agctgctcgc gcgggcgagg
     2641 tgaaagtggt cccgatcaac acgcctcatc tggttccccg cgaccgcaag cccaacgttg
     2701 ccgcctgaga tttctttctc cttgtagtgt gctgttggtt ctaatttggg agcgcttcgg
     2761 cgctcccttc gctttttggg cgcactctca actcccaact ctcaactccc cactctcaac
     2821 tcgccactct caactcccaa ctctcaactc gccttttcac cttcgtggga gggcgccgct
     2881 ctccatccgg cgtctaagat aaacattgac gtttcccccg atggctcgat ccctcccagg
     2941 tcgtcttctg ctcgccagcc tcgcgctcgg cgtggcgggt tgcggcagca tcaactatgc
     3001 tgcgcaggcc ggtgacggca gctttgcact ccgcgcttcg gccaccagcg tagctaccaa
     3061 tggccaggtt gccctccagg cggtcctccc ctccggcgag tccgcgccgg tcgcgtggca
     3121 aatcgcatcc ggaaccaacg ccgcatccct cggacaaggc catatcgatg ccaacggcat
     3181 ctacaccgct cccggctcgc tctcggccga cagcattgcg gtgcgcctcg aagcgacgct
     3241 ctccaacgaa gcgaagagca ccgcctccct cgcggttacg gttcatccag gatttgtcca
     3301 accgctcctg ccgcaaaatg tcgccctcac tccaggcgca acggccgaag tcaccgcgca
     3361 gattgccgaa gtggattctg gcagcgtcag ttggcggctc gccgccacac ccacaggccc
     3421 tgaaaacgca gcgctgggca cgctcagcgg ctccgagtgc cgtcgcagct ccggccgcta
     3481 caccagctgc tcggtcctct ataccgcgcc ttccgcgctt ccagccggtt cggtgtatgt
     3541 cgttgctgct gtcaacggca ccctaaccac cgccccgctc cgcgttctgc tcaacaacca
     3601 gggaattgta tccaacccgg ttacaaatca ggccctccag cagggagcga tcctgctcgg
     3661 cggctccggc ggcaacgacg gcgacttcga cacctatgaa gaccacgatg gcgcgccctt
     3721 catcgccgac tgttgcggcg gaaccctcgg agcgctggtc gaagatcagt ccggcgatca
     3781 gtacatcctc agcaacaatc acgtgctcgc cgaatccgat cagggacaca tcggcgacac
     3841 catcgaccag cccggcctta tcgacggagc ctgcacgccc ctcacacggc ccggatccac
     3901 tctgcgcccc gttggcacac tcgagtcttt tgtcccgctg gcctcgaagc aaaccaacgt
     3961 cgatgcagc
//
LOCUS       DASZYN010000661         2329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_229947, whole genome shotgun sequence.
ACCESSION   DASZYN010000661 DASZYN010000000
VERSION     DASZYN010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229947"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1821)
                     /locus_tag="VHE33_17060"
     CDS             complement(205..1821)
                     /locus_tag="VHE33_17060"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HVT99219.1"
                     /translation="MKKLEIPRRFWGFMEYQPFSITPGDEGFQVNKNLRQKLASSKRR
                     IQRRLDPTDLRGCAKPILTASNIHYEIAERTRGLSAGGIGAIHALATRLGLPQAIDRR
                     LKLLRLHLPYHESDHVLTLAYLPLCGGTCLQDLELLRHDEVFLDALGARRIPDPTTAG
                     DFCRRFTNEHLQTLHDVIDDTRLAVWQQQPASFFRQAIVDMDGFLVETSGQCKRDMDI
                     AYDGTWGYHALVLTLAHTGEVLRVINRSGNRPSQEGAAEQVDQVVALCLRGGFGRVLL
                     RGDTKFSQSERLDGWDDDPRVRFLFGYEAMPNLKALAENLPASAWQPLFRPSPYEVKT
                     QPRRRPDNVKEAVVKARGFENHRLRSEEVAEFRYRPTACKKSYRMVVVRKNIAVEKGE
                     QLLFDRVVYFFYITNLWESEADEIVWSANERCDQENLLAQLHGGVRALRAAVDDLESN
                     GAYMVMTALAWNLKAWWALSLPERPGRWQEKHQAQKRWVLRLEFKSFVNAFVRLPCQL
                     VRTGRKLVFRLLAWNPHQPIFFRLLDVLRC"
     gene            1960..>2329
                     /locus_tag="VHE33_17065"
     CDS             1960..>2329
                     /locus_tag="VHE33_17065"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HVT99220.1"
                     /translation="MEKYRVTLTAEERGHLEHLVSVGKAAARKLTHARILLLADTSQN
                     LSSADEDIVAALGTSLRTVERVRKLLVTEGLQAALHPKPQPPRPDKVKIKGNVEQKLI
                     RLACSDPPQGRCQWTLSLLAD"
BASE COUNT          454 a          722 c          697 g          456 t
ORIGIN      
        1 ccttaaacaa gcgaagattc cgaggtctgc aaggcatctg actcgtggtc gatgacgacg
       61 tatcttctct gccctgccct tggatgggca gttggagttg atccccgttt tgccttgctc
      121 atggtccatt ccccttcctg ctcaacggct ctgattgcca gcacggatcg gagcatccac
      181 actccgactt caagagcgct accttcaaca ccgtaacacg tccaacaggc ggaagaagat
      241 cggctgatgc ggattccagg ccaacaagcg gaacaccaac ttccggcccg tccgcaccaa
      301 ctgacagggc agacgaacga aggcattcac gaacgacttg aactccaacc gtaacaccca
      361 acgcttctgg gcctgatgct tctcctgcca acgacccgga cgctccggca gcgacaacgc
      421 ccaccacgcc ttgaggttcc aggccaacgc cgtcatcacc atgtacgccc cgttgctctc
      481 caggtcgtcc accgccgcac gcaacgcacg caccccgccg tgcaactgcg ccagcaagtt
      541 ctcctggtcg caacgctcgt tggccgacca aacgatctcg tcggcctcgc tctcccaaag
      601 gttggtgatg tagaagaagt acacgacccg atcgaacagc aactgctcgc ccttctccac
      661 cgcaatattc ttccgcacca ccaccatgcg atagctcttc ttgcaggccg tgggccggta
      721 gcggaattca gccacctcct ccgagcgcag ccggtggttc tcgaagccgc gagccttgac
      781 gacggcctct ttgacgttgt ccggacgccg ccggggctgg gtcttcacct cataggggga
      841 tggccggaac aacggctgcc aggccgaggc cggcagattc tcggcgaggg cctttaagtt
      901 gggcatcgcc tcgtagccga acaggaagcg gacgcgcggg tcgtcgtccc agccgtccag
      961 ccgctcgctc tgggagaact tcgtgtcgcc gcgcagcaag acgcggccaa agccgccgcg
     1021 caaacatagt gccactacct ggtccacctg ttcggctgcc ccctcctgcg agggccggtt
     1081 tcccgagcga ttgatgacac gcagcacttc gccggtatgg gccagggtca gcaccagggc
     1141 gtgataaccc caggtgccgt cgtaggcgat gtccatgtcc cgcttgcatt ggcccgaggt
     1201 ctcgaccaaa aagccatcca tgtccacgat ggcttgccgg aagaaggagg ctggttgctg
     1261 ctgccacacg gccagccggg tgtcgtcgat gacgtcatgc agtgtctgca agtgctcgtt
     1321 ggtgaagcgt cggcagaagt cgccagcggt ggtggggtcg gggatgcgac gtgctcccag
     1381 ggcatcgagg aagacctcgt cgtggcggag caactccagg tcttgcaggc aggtgccacc
     1441 gcacaacggc aggtaagcca gggtcagcac gtggtcggac tcgtggtagg gcaggtgaag
     1501 ccggagcaat ttcagccggc ggtcgatggc ctgggggaga ccgaggcgtg tggcgagggc
     1561 gtggatggct ccgatgcctc cggcggagag tccgcgggtc cgttcggcaa tctcgtagtg
     1621 gatgttgctg gcggtcagga taggtttggc gcagccacgt aggtccgttg ggtcgaggcg
     1681 tcgttggatg cgtcgcttgc tgctggccag tttttggcgt aagttcttgt tcacttgaaa
     1741 tccttcgtcg cctggagtga tggagaacgg ttgatactcc atgaaacccc agaaacgccg
     1801 agggatttcg agttttttca caattcgctt cggcccaagg cttgtgactt cgcttgcata
     1861 aggcctagta tgccgtcaat cttcgattga cgggtaaagt gagatcctgt ttcttgactt
     1921 tcggagccaa gtgcatccct caagaacagg aggtccatca tggagaaata tcgggtcact
     1981 ttgacggcag aggagcgagg acacttggag cacctggttt ccgtcggcaa ggcagcggct
     2041 cgcaaactta cccatgcccg cattctgctc ctcgctgata cgtcccagaa cctttcctcc
     2101 gctgacgagg acattgtcgc cgctcttggc accagcctgc ggacggtcga acgggtgcgg
     2161 aagctactgg tcaccgaagg gttgcaggcg gctctccacc ccaagcccca accccctcga
     2221 ccggacaagg tcaagatcaa ggggaacgtt gagcagaagc ttatccgcct ggcttgcagt
     2281 gatccaccgc agggacgttg ccaatggacc ttgagcttgc tggcagacg
//
LOCUS       DASZYN010000662         3753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_613834, whole genome shotgun sequence.
ACCESSION   DASZYN010000662 DASZYN010000000
VERSION     DASZYN010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613834"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VHE33_17070"
     CDS             <1..342
                     /locus_tag="VHE33_17070"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HVT99221.1"
                     /translation="ATVMAIVGFALTFHHRAALTPRNTALLVAMGILCSAGSLCFFFA
                     LKHTTAANVSQFHYTQLITGALMAWLVWRQIPTLFTLTGAVLIIVSGVYIALHASRDT
                     RPWTHPTTVPD"
     gene            complement(352..1155)
                     /locus_tag="VHE33_17075"
     CDS             complement(352..1155)
                     /locus_tag="VHE33_17075"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT99222.1"
                     /translation="MTNVSDERMQATRVLNGKTAIVTGGASGIGLAIARAFAREGAAV
                     SIVDQNESGAAEAAREIVGAGGTAQGVRCDVSDSGSVQAAFGSVFARGAVHILVNCAG
                     VAHVGTATTTSEDDFDRILRINVRGVYLCTRAVLDAMKRQGGGIILNMASIAGMAGLP
                     DRFAYSTSKGAVLAMTYSVARDYIKDGIRCNAISPARVHTPFVDGFVAKNYPGREAEM
                     MEKLGESQPIGRMAQPDEVASLAVWLCSDASSFVTGANYPLDGGFINVR"
     gene            1250..2551
                     /locus_tag="VHE33_17080"
     CDS             1250..2551
                     /locus_tag="VHE33_17080"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HVT99223.1"
                     /translation="MNDLRISGVQVIDLRFPTSRESVGSDAVNKDPDYSAAYCILETN
                     HGLRGFGLTFTLGRGTELCCEALRYLSRCVTGRTLSSITDDFCAFSRELTGDTQFRWL
                     GPEKGVIHLATAALINAVWDLYARAENKPLWKLLADMEPERIASVIDFHYISDALPRE
                     EAIAMLTDRRRDQAKRLEHLSNVGLPAYTTSAGWIGFSDEKIARLCDEGLADGWTHFK
                     LKVGGDPDDDRRRAALMRSKIGESNRLMMDANQKWDVEEAIQRTRELQEFNPWWMEEP
                     TSPDDVLGHARIRREVPGVRIATGEHCQNRILFKQFMQAQAMDVCQIDSCRLAGVNEN
                     IAVILLAAKFGIPVCPHAGGVGLCEYVQHLSAFDYLCVSATLENRVVEFVDHLHEHFV
                     DPVRIRRGRYLLPAQPGYSIEIFPETLRDFSYPDGRVWRSA"
     gene            2548..3396
                     /locus_tag="VHE33_17085"
     CDS             2548..3396
                     /locus_tag="VHE33_17085"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HVT99224.1"
                     /translation="MKLVSFQFFSEPTPRPGLLLNDAILDLSPLGVPDAVSTIAVGTT
                     ALHQALEMPGTSLIALSAVHLLAPVPRPARIFCVGLNYRDHAIESKMEIPKVPTIFIK
                     LPSAVIGPNEPVRIPALTKQPDYEVEFAIVIGKAGRNIAKENWQEHVFGYTILNDVSA
                     RDVQLATSQWTLGKSFDTFCPIGPAIVTQDEIADPHNLDIRLSIGGEVLQHSNTRELI
                     FKAPDLISYISSVTELQPGDIISTGTPAGVGLGRSPQRWLQPGETMTAEIEGLGQLIN
                     PVVAAD"
     gene            complement(3398..>3753)
                     /locus_tag="VHE33_17090"
     CDS             complement(3398..>3753)
                     /locus_tag="VHE33_17090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99225.1"
                     /translation="QVAEFGAKMYFLHKAFPQIVGPEYTLRSANYLLGTHPVSSTSYI
                     SSVGTVSKMKAYGNNRADNTFIPGGMIPGYIIIKPDFPECIDDFGMLWFENEYTISTT
                     AAWILEANAADALTR"
BASE COUNT          728 a         1468 c          887 g          670 t
ORIGIN      
        1 gcgactgtca tggccatcgt cggattcgcg ctcaccttcc accaccgcgc cgcccttacg
       61 ccccgcaaca ccgctttgct cgtcgctatg ggtatccttt gttccgccgg gagcctctgc
      121 ttcttcttcg cgctcaaaca caccaccgcc gccaacgtct cgcagttcca ctacacgcag
      181 ctgatcaccg gtgccctgat ggcatggctg gtctggcgtc agataccaac gttgtttacg
      241 ctgaccggcg ccgtcctcat catcgtctca ggcgtgtata tcgccctgca cgcctcccga
      301 gacacgcgcc cctggaccca ccccacaacc gtcccggact aagccctaac ctcaccgcac
      361 gttgataaac ccgccgtcca gcggataatt cgcccccgtc acaaagctcg acgcatccga
      421 gcacagccac accgccagac tcgccacctc atccggctgc gccatccgcc caatcggctg
      481 cgactcgccc agcttctcca tcatctcggc ctcgcgcccc ggataattct tcgccacgaa
      541 cccatccaca aacggcgtgt gcacacgcgc cggcgaaatc gcgttgcagc gaattccgtc
      601 ctttatatag tcccgcgcca ccgagtacgt catcgccagc accgcgccct tgctggtcga
      661 atacgcaaaa cgatccggca accccgccat ccccgcaatc gacgccatgt tgaggatgat
      721 cccgccgccc tgccgcttca ttgcatcgag caccgcccgc gtgcacagat agaccccgcg
      781 cacattgatc ctcaggatgc ggtcaaaatc gtcctcgctc gtcgtcgtcg ccgtgcccac
      841 atgcgcaacg cccgcgcaat tcaccaggat gtgcaccgca ccgcgcgcaa aaacactccc
      901 gaacgccgcc tgcacgctgc ccgagtcaga aacgtcgcac ctcacgccct gcgctgttcc
      961 cccggcacca acaatctcgc gcgccgcctc tgccgcgccg ctttcgttct ggtccacaat
     1021 cgagaccgcc gctccctccc gagcaaacgc ccgcgcaatc gccaggccga tgccgcttgc
     1081 gccacctgta acgatggccg tcttgccatt cagaacccgc gttgcctgca tccgctcatc
     1141 gctcacgttc gtcacaacct caatccccga aaattgtagt tcccttctgc acccgccagc
     1201 ccacaatgcg ggacacgaca cggcccccct cgttatcctc aaagaaccag tgaatgatct
     1261 ccgcatctcc ggcgtccagg tgatcgacct tcgctttccc acatcgcggg agagcgtggg
     1321 gtccgacgcc gtcaacaaag atcccgacta ctcggcagcc tactgcatcc tcgagaccaa
     1381 ccacggtctc cgcggcttcg gcctcacttt tactttgggc cgcggcaccg aactctgctg
     1441 cgaagcgctg cgctacctct cccgctgcgt caccggccgc accctcagct ccatcactga
     1501 cgacttctgc gccttcagcc gcgagctcac cggcgacacc cagttccgct ggctcggtcc
     1561 cgaaaaaggc gtcatccatc tcgccactgc cgccctcatc aacgcggtct gggacctcta
     1621 cgcgcgcgcc gaaaacaagc cgctctggaa gctcctcgcc gacatggagc ccgaacgcat
     1681 cgcctccgtc atcgacttcc actacatcag cgatgccctc ccgcgcgaag aagccatcgc
     1741 gatgctcacc gatcgtcgcc gcgatcaggc gaagcgcctc gaacatctca gcaacgtggg
     1801 tctccctgcc tacaccacct ccgccggatg gatcggtttc agcgacgaaa agatcgcccg
     1861 cctctgcgac gaagggctcg ccgacggctg gacccacttc aaactcaaag tcggcggcga
     1921 cccggacgac gaccgtcgcc gcgccgctct catgcgttcc aaaattggcg aatcgaatcg
     1981 cctcatgatg gacgccaacc agaagtggga cgtcgaagaa gccatccagc gcacccgcga
     2041 actccaggag ttcaatccct ggtggatgga agagcccacc agccccgacg acgtgctcgg
     2101 ccacgcgcgc atccgccgcg aagtccccgg cgtccgcatc gccaccggcg aacactgcca
     2161 gaatcgcatc ctcttcaagc agttcatgca ggcccaggcc atggacgtct gccagatcga
     2221 cagctgtcgc ctcgccggcg tcaacgagaa catcgccgtc atcctgctcg ccgccaaatt
     2281 cggcattccc gtttgccccc acgcaggcgg cgtcggtctt tgcgaatacg tgcagcatct
     2341 ctccgccttc gactacctct gcgtctccgc aaccctggaa aatcgcgtcg tcgaattcgt
     2401 cgaccatctg cacgagcact tcgtcgaccc cgtccgcatc cgccgcggcc gctacctgct
     2461 ccctgcccag cccggctaca gcatcgaaat cttccccgaa actctgcgcg acttcagtta
     2521 tcccgatggc cgcgtctgga gaagcgcatg aaactcgtct ccttccagtt tttttccgaa
     2581 cccaccccgc gtcccggcct gcttctcaac gacgccatcc tcgacctgtc cccgctcggc
     2641 gtccccgacg ccgtcagcac catcgccgtc ggaaccactg ccctccatca ggcgctggaa
     2701 atgccaggca cctcgctcat cgcgctgtct gccgtgcatc tcctggcccc tgtcccccgc
     2761 cccgcgcgta tcttctgtgt cggcctcaac taccgcgacc acgccatcga atcaaagatg
     2821 gaaattccga aggttcccac catctttatc aagctccctt cggccgtcat cggtcccaac
     2881 gagcccgtcc gtatccctgc gctgacaaag cagcccgact acgaagtcga atttgccatc
     2941 gtcatcggca aagccggccg caacatcgcg aaggaaaact ggcaggaaca cgtcttcggc
     3001 tacacgattc tcaacgacgt cagcgcgcgc gacgtgcagc tcgccacatc gcagtggacc
     3061 ctcggcaaaa gtttcgacac cttctgcccc atcggtcccg ccatcgtcac ccaggacgaa
     3121 atcgcggacc cgcacaacct cgacatccgc ctttccatcg gcggcgaagt cctgcagcac
     3181 tccaacacgc gcgagctcat cttcaaagca ccggacctga tcagctacat ctcatccgtc
     3241 accgaactcc agcccggcga catcatcagc accggcaccc ccgccggagt cggcctcgga
     3301 cgcagccccc agcgctggct gcagcccgga gaaaccatga ccgccgaaat cgaaggcctc
     3361 ggccagctca tcaatcccgt cgttgccgcc gactagctca ccgcgtcagc gcatcggccg
     3421 catttgcctc aagaatccac gccgccgtcg tgctgatcgt gtactcgttc tcaaaccaga
     3481 gcatgccgaa gtcgtcgatg cactccggaa aatccggctt gatgatgatg tagcccggaa
     3541 tcattccccc cggaataaac gtattgtcgg cgcgattgtt cccgtacgcc ttcatcttcg
     3601 acaccgtgcc caccgacgaa atgtacgacg tgctcgacac cggatgcgtc cccagtaaat
     3661 agttcgccga tcgcagcgtg tactccggcc ccacaatctg cggaaatgcc ttgtgcagga
     3721 agtacatctt cgccccaaac tccgccacct gcg
//
LOCUS       DASZYN010000663        10513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_292236, whole genome shotgun sequence.
ACCESSION   DASZYN010000663 DASZYN010000000
VERSION     DASZYN010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 10513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10513
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292236"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1442)
                     /locus_tag="VHE33_17095"
     CDS             complement(39..1442)
                     /locus_tag="VHE33_17095"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HVT99226.1"
                     /translation="MQTSPDRTAPSAIPDLPRVLNTSHATAIVVGTIIGSGIFLVPQE
                     MMRATGSSSLVYLAWIAGGLLSLFGAMTYAELSTLQPYAGGEYVYIRGVYGDLPAFLY
                     MWTWFAVAKPASIASVTTGLARTLAIFPALHWLDGVAFHAGFAVLWSQVFAIAATWLM
                     TGLNYLGIRKAGDFQLIFTWLKGVLIVAIAVICFGSLGGHWGNFATTFVGAHGGFGGF
                     MIALIAALWAYDGWNDLTMVAGEVRNPGRSLPVALIAGLGIVGILYMATNAAIQYILP
                     ATQIAASPRPAVTALQAITGPWGAALVSIAMAVSIFVTLNGTIMSGARVPFAAARDGL
                     FFRRMADVHPRFQSPSTSLIVQALLTTGLLLAVGRFQQLFELAIFAEWLFYLITATTI
                     FVYRRRETDEKIYHVWGYPVIPALFIAAAGVLLVYSYAENLRNSLIGTAIILLGVPLF
                     LVFRKQARTSAAGGSVR"
     gene            complement(1455..3068)
                     /locus_tag="VHE33_17100"
     CDS             complement(1455..3068)
                     /locus_tag="VHE33_17100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HVT99227.1"
                     /translation="MSTLTVNNAITAEREQSFRQFVWTCLAMAALKLAIQIVGNILAQ
                     HAGYGIFRDEMYYLICGRHLAFGYVDQPPMVALQARLTDILFGYEHMWSLRLLSAMAG
                     AFKVFLAGALVWVFGGNRRAAFLAMLGVIVAGVYLGIDSFLSMNSFEPVFWIPCALAL
                     VRISQEHGIPGREAVVRNWWIVLGLSAGLGLENKWNEVFFLVAMLVALLLTPQRRILA
                     SRWFGVAMALMVALALPNLLWEAHNHWPTLEWLVGVSKSNKDVKLPPLQFIAGQIMML
                     NPFTSPLWISGVLWLLFGRAARPFRFLGVLYPVFLLIMMALHAKDYYLAPIYVIYYSA
                     GAVCWLSGLRVPMWRNVLGGAYALLLGFAFAQGVPFSIPILPPPKFIAYEKKIGFTPK
                     DTENHDATILPQFYADRFGWHEMVEKVARIYNSLPPQERAVTGIYTGNYGEASAINLF
                     GPKYGLPVAISGHQSYWLWGPQGYTGQEMIIINGAKLEDMKQSYQSCLIADRMENPLS
                     MPWEIKPIYLCRGRKSTYAADWKDFKFYY"
     gene            3179..3847
                     /locus_tag="VHE33_17105"
     CDS             3179..3847
                     /locus_tag="VHE33_17105"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HVT99228.1"
                     /translation="MIVIFQDRRHAGQALARLVHLESADDLASGDGIVLGLPRGGVPV
                     AFEVARELRLPLDVFVVRKLGVPGQEELAMGAVASGGVIVLQKDVIAAYRIDQETIDK
                     AVAREHLEIKRRENAFRQGRPPLDLRCHTIILVDDGLATGATMKAAVRALRPIARRMI
                     VAIPVAASGTCEEVRKLVDRLICIECPEFFHAVGEFYRNFDQTTDDEVCSLLAQARQE
                     QPIT"
     gene            4015..4152
                     /locus_tag="VHE33_17110"
     CDS             4015..4152
                     /locus_tag="VHE33_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99229.1"
                     /translation="MDHLTVGLGTVWIVFTIIATALLGTDFILSEIRSTIRNHRESET
                     K"
     gene            4236..4312
                     /locus_tag="VHE33_17115"
     tRNA            4236..4312
                     /locus_tag="VHE33_17115"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4270..4272,aa:Val,seq:tac)
     gene            4442..5542
                     /gene="rodA"
                     /locus_tag="VHE33_17120"
     CDS             4442..5542
                     /gene="rodA"
                     /locus_tag="VHE33_17120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HVT99230.1"
                     /translation="MRRFLSLRDFDWTLLAFVLLLSVISVFEIYSATLHTKFKGFEHK
                     QILWIAGGLLAMFVMSVIDYHKLLTAVYWIYGFCLVGLVAVLAIGTRVNGGKRWIKLP
                     GGAHFQPSEWVKLVLILLVARYFNNLAGRELSWKDIFKAIGFVALPMLLVLKQPDMGT
                     ALTYTPVLLVGLFLGGIDWKKGGILVLVTVLAVGGIWKSGKILKPYQKARLTSFMNPD
                     ADPRGSGYQILQSKIAVGDGGVFGRGANRGTQTQGDFLPIPYTDFIFAAFSEEHGFVG
                     AILVLLIYFLILMRLIQNAQTAADLPGSLLVMGVAAVLIFQIAVNVGMVLGLMPVTGI
                     PLPLMSYGGSSVLFTFLALGMVMNVRMSRFVN"
     gene            5878..8874
                     /locus_tag="VHE33_17125"
     CDS             5878..8874
                     /locus_tag="VHE33_17125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HVT99231.1"
                     /translation="MAKEICISSTPHETRLAILEDDQLSEIYYERENEYTLAGSIYKG
                     RVTRVLPGMQSAFVDLGLERDAFLYVTDFLEEQEEDSADFERSDSTGNGRRGREPREH
                     REPREAREPREAREPREARPATPEPEGDLSASEGAPRRGDRGRERSDRGERGGEGGAR
                     RWHGRRGRRFRGRGPRAEQSSLDSPEIVEEATAAEPVALPERGAPGGMPAQNLPIVLP
                     GESLSKYGGGGESKQTSKAPSSSGPAAARGGSAKPSTMVEVPTGWDGGSVLPGETLSR
                     HRGGRSSGNGQPIEVQSTGAEPAVVTSAPEESLVAVVPAPQTLPVETLDTAAPPLPES
                     APSHTESIQHDVEDVATQELGIDTVEEEQEYEPEDASVSHRVETPRSEFRFAPVEEEE
                     EIEEEEVEPPVPFESQEPESVAEVTHSAEPAAAASSEVNSAESEVVRQLFGYAPGMGV
                     LEEETIDEDEYDFEPIPGQPDEHMHEELEEETLDQGSTLDEAVRDVHIEKRLGYGTPA
                     EAEEDEAGEETEEAETGFAEGEEEDFHESEQESDLGEEVAEGAEASGEPAAAAPGRRE
                     DRRFGRRGGGRGGRQPGRRHGGRRPSMQTSNLPIISELLKQGQEILVQIAKEPIARKG
                     ARITSHIALPGRFLVFMPTVNHVGVSRKIASDEERQRLKRVLIGEKGDAPGGFIVRTA
                     AESASDDDLRADIRFLINLWTEIKQRSDSAKAPALIYHDLNLIERILRDQVSDNFATI
                     WVDSESEYERIVRFLNRFSPSLVRRVKLYTKETPLFEQFGIQEEITKALKSKVWLKSG
                     GSIVINQTEALVAIDINTGKYVGKTARLEDTILKTNLDAIPEIVRQIRLRDLGGIIVI
                     DFIDMDERRNRQKVTQALEDALKADRAPSKVLQFNDFGLVAITRKRVKQSLERTLSVP
                     CPVCTGTGMVKSPITVANELWIEMRKMMRHFERGDVMLRVHPEVVKVLKANNAKWLNE
                     LEEMIGKTILVKSDPALPPEQFDIQG"
     gene            9090..10013
                     /locus_tag="VHE33_17130"
     CDS             9090..10013
                     /locus_tag="VHE33_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99232.1"
                     /translation="MIANCDRHRALLRRVVTTAFAVAVGVSLNASAQTSLPADHAANG
                     QSYSSSNNYQAYLSTDSLLGGSSYAEPSGGASASPQYGGNRRSSNYPSYESRWSHFAF
                     NAGAGFTIPIGNTTSFNRAEAEDPNGPLSPSQGLGYNVDLGAGWNFSKRFGVLLDYEF
                     LRDSIPNDYTDILAGGSGTGLGGNINTWSLALDPIIYLPMSRKSGAYVTGGGGFYRKV
                     TNFTEPVEECDYYYGCYGVPVTVEHFSSNQGGANFGIGFYHKLFGEDSNGKFYAQVKY
                     VFVNSPRPSTSNFGQGSGTEELLPVTFGVRF"
     gene            complement(10285..>10513)
                     /locus_tag="VHE33_17135"
     CDS             complement(10285..>10513)
                     /locus_tag="VHE33_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99233.1"
                     /translation="GAGDPEWARTLPPPQWFSPEDGLITICTLHDFLKEAPTALGADT
                     TRVVSELEEYERVLRKTAVRNLRWQLAVSWK"
BASE COUNT         2174 a         3215 c         3113 g         2011 t
ORIGIN      
        1 tgcggacgaa ggaattcttg ttcaagtgaa tcgaggggct atcggacgga accgccggct
       61 gccgaggttc gggcctgttt tctgaataca aggaagagcg gaacccccag caagatgatt
      121 gcggtcccga tcagtgagtt ccgtaggttc tctgcgtacg agtagacgag cagcacgccg
      181 gccgctgcga tgaacagagc cggaatcacc ggataacccc acacgtgata aattttttcg
      241 tcggtctcgc gacggcgata aacgaagatc gttgtggccg taatcagata gaacagccac
      301 tcggcgaaga tcgccagctc gaacaactgc tggaaccgtc cgacagcaag aagcaatccc
      361 gtcgtcagca gcgcctgcac aatcagggaa gtggacgggc tctggaatcg cggatgcaca
      421 tccgccatcc tccgaaagaa cagcccgtca cgtgctgcgg cgaagggcac gcgtgcgccc
      481 gacataatgg ttccgttcag cgtgacgaag atgctcaccg ccatggcgat cgagaccagg
      541 gctgcgcccc acgggcctgt gatcgcttgc agcgccgtca ccgccggcct cggcgaagcc
      601 gcgatctgcg ttgcgggcag gatgtactgg atcgctgcgt tcgtggccat gtagagaatc
      661 ccgacgatcc cgaggccggc gatcagcgct acggggaggc ttcgtcctgg attccgaact
      721 tcgccggcga ccatggtcag atcattccag ccgtcatagg cccacagggc cgcgatgagc
      781 gcaatcatga agccaccgaa tccgccgtgt gcccccacaa acgtcgttgc gaagttcccc
      841 caatgtccgc ccagcgaccc gaaacagatt acggcaatgg ccacgatcag cacgcccttc
      901 agccaggtga aaatcagctg gaaatcgccg gctttgcgaa tgccgaggta gttgagccct
      961 gtcatcagcc atgtcgccgc gatggcaaaa acctgcgacc acagcaccgc gaacccggca
     1021 tgaaacgcga cgccatccag ccagtgcaac gccgggaaga tcgcgagcgt tcgcgccagt
     1081 cccgttgtca cgctggcaat ggaggcaggt tttgcgacgg caaaccaggt ccacatatag
     1141 aggaatgccg gcaaatcgcc gtagacaccg cgaatgtaaa catactcgcc gccggcatag
     1201 ggctgcagcg tgctcagttc cgcataggtc atcgcgccga agagcgagag caggccgccg
     1261 gcgatccacg ccaggtagac caggctcgac gaacccgtcg cgcgcatcat ttcctgggga
     1321 acgagaaaaa ttccgctgcc gatgatggtg ccaaccacga tcgcggtagc atgcgatgtg
     1381 ttcaggaccc ggggcaggtc gggtatggca ctcggagcgg tccggtcagg cgaggtttgc
     1441 agggatgaaa gacctcagta gtagaacttg aagtccttcc agtctgccgc atacgtcgat
     1501 tttctgccac ggcaaagata gatcggcttg atctcccatg gcatcgaaag gggattttcc
     1561 atccgatccg cgatcaggca cgactggtag gactgtttca tatcctccag cttcgcgcca
     1621 ttaataatga tcatctcctg cccggtgtat ccctgcggtc cccacagcca gtagctctgg
     1681 tgtccgctga tcgcgaccgg aaggccgtac ttcggaccga acaaattgat cgcgctggcc
     1741 tcgccgtaat taccggtgta aatgccggtg acggcgcgct cctgtggggg aagggaattg
     1801 tagatgcgcg ctaccttctc caccatctcg tgccagccaa agcggtccgc gtagaactgc
     1861 ggcaggatgg tagcgtcgtg attttccgtg tcttttggtg taaacccgat ctttttctca
     1921 taggcgatga acttcggcgg cggcaggatc ggaatggaaa acggaacgcc ctgagcaaat
     1981 gcgaagccaa ggagcaaggc gtacgctccg cccagaacgt tgcgccacat cggcacgcgg
     2041 agacccgaca gccagcagac ggcgcccgca gagtagtaga tgacatagat cggtgcgagg
     2101 tagtagtcct tcgcgtgcag agccatcatg atgagcagga acaccggata aagcacgcca
     2161 agaaaacgaa acggacgcgc cgcccgccca aacagcagcc acagcacgcc gctgatccac
     2221 agtggggaag tgaacgggtt gagcatcatg atctgtccgg cgatgaattg cagtggcggc
     2281 agcttcacat ccttattgct cttcgaaacc cccaccagcc attccagcgt cggccagtgg
     2341 ttatgcgcct cccacaatag attcggcaag gccagcgcca ccatcagggc catggcaacg
     2401 ccgaaccagc ggctcgccag gattcgccgc tgtggcgtca gcagcagggc gaccagcata
     2461 gcgacgagga aaaagacctc gttccattta ttttccagcc ccagtcccgc gctaaggccg
     2521 agcacgatcc accaattgcg gacgacggct tctcgtcccg ggattccatg ctcctgggaa
     2581 attctgacca gcgccagcgc gcaggggatc cagaacacgg gttcgaagga attcattgac
     2641 aggaagctgt cgattccgag atagacaccc gcaacgatca cgcccagcat ggccagaaat
     2701 gcggctcgcc ggttgcctcc aaacacccag acgagtgcgc cggcgaggaa gaccttaaag
     2761 gcgcccgcca tcgccgaaag caaccgcagc gaccacatgt gttcgtaccc gaagaggatg
     2821 tccgtgaggc gggcctgcaa ggccaccatc ggcggctgat cgacgtagcc aaaggcgagg
     2881 tgccggccgc aaatcaggta gtacatctca tcgcggaaga ttccataccc ggcgtgctgc
     2941 gccagaatgt tgccgacgat ctggatagcg agtttgagag ccgccatcgc gaggcaggtc
     3001 cagacaaact gtcgaaaact ctgttcccgc tccgccgtta ttgcgttgtt gaccgtgagt
     3061 gtgctcatgc gagagatgag acggggactc ctccttcaga atacgcagag ctccggcaaa
     3121 cgttccgtga aaccacttgc ataaccggtc ttccgggcgt ccaatcgttc acggagccgt
     3181 gatcgtcatc tttcaggatc gccgtcacgc tggccaggcg ttggcgcggc tggtccacct
     3241 tgaatctgcg gacgaccttg cgagcggaga cgggatcgtt ctcgggctgc cgcgcggtgg
     3301 ggttcccgtt gcttttgaag tggcgcgcga gcttcgtttg cctctcgatg tcttcgtcgt
     3361 gcgtaagctg ggcgttccgg gccaggaaga gctcgcgatg ggtgcggtgg ctagtggcgg
     3421 ggtcatcgtg ctgcagaagg atgtcattgc ggcgtaccgc atcgatcagg aaaccatcga
     3481 caaagcagtg gcccgcgaac atctggagat caagcgccgt gagaacgcct tccggcaagg
     3541 gcgcccgcca ctcgatttgc ggtgtcacac gatcattctc gtcgacgatg gcttggcgac
     3601 gggcgccacg atgaaagcag ccgtgcgcgc tctgcgaccc atcgcccgcc gcatgatcgt
     3661 ggcgattccc gtggccgcat ctggcacgtg cgaagaagtg cgcaagctcg tggaccgcct
     3721 gatctgcatc gaatgcccgg aattctttca cgcggtcggc gaattctatc gcaatttcga
     3781 tcagacgacg gatgacgagg tatgttcttt gctggctcag gcgcgtcagg agcaacccat
     3841 cacatagctt gggagtcgcg cactgtcgta accggtcgca gggcgagggg atccacggag
     3901 tgggcctgac cccagagttc gggcggcctc cacccacgtt gtatgggcca aagagccacc
     3961 gctgcttaca ctgaagttcg taccagagta agaccagtgt gacgggatcg tggtatggac
     4021 catttgacgg ttgggctggg aacggtttgg attgtgttca ccatcattgc taccgcgcta
     4081 ttgggaacgg acttcatcct gagcgaaatt cgcagcacca tccgcaacca tcgcgagtcc
     4141 gagacaaaat agcttcccca tacgaagagt gctgcaccga ctgctaaaat tgaccggaag
     4201 ccccgatgag ccgcgatggc ggcgctgccg gttgcgggcg gttagctcag ctggtcagag
     4261 cgcctgcctt acaagcagga ggtcgcaggt tcgagccctg cactgcccac caatccccag
     4321 tctgcagggg gattctccga tccgcgcgga gcactcgttt acgctcccaa atgcaataat
     4381 gacagcaaga ttccctttga gtgatttcct ctggacctct tcagcgggga acccgtagtt
     4441 tatgcgccgc ttcctctccc tccgcgattt cgactggacg cttctcgcct tcgtcctgct
     4501 tctctccgtc atcagcgtct tcgaaattta cagcgcaacc ctgcacacta agttcaaggg
     4561 cttcgagcac aagcaaatcc tctggatcgc cggcggcctg ctggcgatgt tcgtcatgtc
     4621 ggtgatcgac taccacaagc tcctcactgc ggtttactgg atttacggct tctgtctcgt
     4681 ggggctggtc gcggtgcttg ccataggcac ccgcgtgaac ggcggcaaac gatggatcaa
     4741 acttcctggc ggcgctcact tccagccttc cgagtgggtc aagctggtgc tcatcctcct
     4801 cgtcgcgcgc tactttaata acctcgctgg ccgcgaactc agctggaagg acattttcaa
     4861 ggctatcggt tttgtcgcgc ttcccatgct gctcgtcctc aagcagcccg atatgggcac
     4921 ggccctcacc tacaccccgg tcctgctggt gggcctcttc ctggggggca tcgactggaa
     4981 aaaggggggc atcctcgttc tggttaccgt gctcgccgtc ggcggcatct ggaaaagcgg
     5041 caaaatcctc aagccctacc agaaggctcg cctcaccagc ttcatgaacc ccgatgccga
     5101 cccgcgcggc agtggctatc aaatcctgca atccaagatc gccgtcggcg acggcggcgt
     5161 cttcgggcgg ggcgccaatc gcggtaccca gacccagggc gacttcctcc ccatccccta
     5221 caccgacttc atttttgccg cttttagcga ggaacacggc ttcgtgggcg cgatcctcgt
     5281 gctcctgata tactttttga tactgatgcg tttgattcaa aacgctcaga cagccgcaga
     5341 cctgccaggt tcgctcctgg taatgggcgt ggcggcggtc ctcatttttc agatcgccgt
     5401 taacgtcgga atggttttgg ggctgatgcc ggtgaccggc atccctcttc ctcttatgag
     5461 ttatggggga tcctcggtac tgttcacctt tctggctctg ggcatggtaa tgaacgtccg
     5521 catgagccgc ttcgtgaact aacgcggcac aggtttagca cgcagggccc gcagggcggg
     5581 cgcagtttga acgaagttta ctaaccggcc aggtggatcg aaacgggccg ggcaggatca
     5641 ggaatggacg cgaaccgatg gctaacgacc ccgccatgca gggctgaagc ggtcacatct
     5701 tcgcgcagga acgactctcc ggaaagaatg caaattccca gcgacatctc cccaaacctc
     5761 tcccctcacc cagggcgagg cggcacgggc ttgtgcgcgc agactcccgg actctcctcc
     5821 ttcactcccg atcttctccg gtgacccgct cgttccgccc tggcattttg aggcggaatg
     5881 gcaaaagaaa tttgcatctc cagcacgccg cacgaaacgc ggcttgcgat tctcgaagac
     5941 gatcagctct cggagatcta ctacgagcgc gaaaatgagt acacactagc cggctctatc
     6001 tacaagggcc gcgtcacccg ggttcttccc ggtatgcagt ccgcatttgt cgaccttgga
     6061 ctggaacggg acgctttcct ctacgtcacc gattttctcg aagagcagga agaggattcg
     6121 gccgatttcg agcgcagcga ttccaccggt aacggccgtc ggggccggga gccgcgtgaa
     6181 catcgcgaac cccgcgaagc gcgggaacct cgtgaggcgc gggagcctcg tgaagctcgt
     6241 ccagccaccc ccgaaccaga gggcgatcta tccgcatccg agggcgcgcc acggcgcggt
     6301 gatcggggcc gagagcggtc ggatcgcggt gagcgcggcg gagaaggcgg cgcacgccgc
     6361 tggcacgggc gtcgtgggcg acgtttccgc gggcgtggtc cccgcgccga acagagttcg
     6421 ctcgactcgc cagagattgt ggaagaggcg acagcggccg aaccggtagc acttcccgaa
     6481 cgcggagctc ccggcggcat gcccgcacaa aacctcccca ttgttcttcc cggcgagtcg
     6541 ctttcgaaat acggcggggg tggggagtcg aaacagacat cgaaggcccc atcgagcagt
     6601 ggcccggcag cagcccgtgg cggcagcgcc aaaccttcca ccatggtcga agtgccgacc
     6661 ggctgggacg gtggctctgt gctgcccggc gaaactcttt cccgtcatcg cggtggccgt
     6721 tcatcaggca acggtcaacc gatcgaggtg cagtccactg gagctgagcc ggccgtagtg
     6781 acgtccgccc cggaagagtc acttgttgct gtggttcccg ctccccagac cctgccggtc
     6841 gagacgctcg atacggcggc gcctcccctg ccggaatctg cgccttctca cacagaatcg
     6901 atccagcacg acgtcgagga cgtcgccacg caggaactcg gcatcgatac ggtcgaggaa
     6961 gagcaagagt acgaacctga ggacgcctct gtttctcatc gtgtggagac gccgcgcagt
     7021 gagttccgct ttgctcccgt tgaggaggag gaggaaattg aagaggaaga agtcgagccg
     7081 cctgtgcctt tcgaaagcca ggagcccgag tcggtcgccg aggtaacgca ttccgctgag
     7141 cccgcagcgg ccgcgtccag cgaggttaac tcggctgaga gcgaagtcgt ccgccagctc
     7201 ttcgggtacg cgcccgggat gggcgttctc gaagaggaaa ccatcgatga ggatgagtac
     7261 gactttgagc ccatccccgg ccagcccgac gagcacatgc acgaagagct ggaagaggaa
     7321 actctggatc agggttcgac gcttgacgaa gccgtacgcg acgttcacat cgagaaacgc
     7381 ctgggctacg gcactcccgc agaagctgag gaggacgaag caggggaaga gacggaggag
     7441 gccgaaaccg gattcgctga aggtgaagaa gaggacttcc acgaatccga gcaggaatcc
     7501 gatctcggtg aagaggtggc cgagggcgct gaagcatccg gggagcccgc tgccgctgct
     7561 ccgggtcgcc gcgaggatcg ccgcttcggc cgccgtggcg gtggtcgagg aggacgccag
     7621 cccggtcgcc gccatggtgg acgccgtccc agcatgcaga catcgaatct gcccatcatc
     7681 agcgagttgc tgaaacaggg acaggaaatt ttggtgcaga tcgcgaagga gccgattgcc
     7741 cgcaaaggcg ctcgcattac cagccatatc gcgcttcccg ggcggtttct cgtcttcatg
     7801 cccaccgtca accacgtcgg tgtctcccgc aaaatcgcct ccgatgaaga gcgtcagcgg
     7861 ctgaagcggg tgctcatcgg cgaaaagggc gatgcccccg gcggatttat cgttcgcact
     7921 gcggccgaaa gcgccagtga cgacgatctg cgagccgaca ttcgcttcct catcaacctc
     7981 tggaccgaga tcaagcagcg ttccgactct gctaaagccc cggcgctgat ctaccatgac
     8041 ctcaacctga tcgagcgcat cctgcgcgat caggtcagcg acaacttcgc caccatctgg
     8101 gtcgattcgg aatcggagta cgagcgcatt gtccgcttcc tgaatcgctt ttcgccctcg
     8161 ctcgtccggc gcgtcaagct ctacaccaaa gagacgccac tcttcgagca gttcggcatt
     8221 caggaagaaa tcaccaaggc gctgaagtcc aaggtctggc tcaagtccgg tggttcgatc
     8281 gtgatcaatc agaccgaggc tctggtcgcg attgacatca acaccggcaa atacgttggc
     8341 aaaacggcgc gtcttgagga caccattctc aagaccaacc tcgatgccat cccggaaatc
     8401 gtgcgccaga ttcgtctccg cgatctgggc ggcatcatcg tcatcgattt catcgatatg
     8461 gacgaacgcc gcaaccgcca gaaagtgacc caggccctcg aagacgcgct taaggccgac
     8521 cgcgccccat cgaaggttct ccagttcaac gatttcggac tggtggccat cacgcgcaag
     8581 cgtgtcaagc aatcgctcga gcggaccctc agcgttccgt gtcccgtttg caccggcacc
     8641 ggcatggtga agagcccgat caccgtcgcc aacgagctct ggatcgaaat gcgcaagatg
     8701 atgcgccact tcgagcgcgg tgacgtcatg ctgcgcgttc atccagaagt cgtcaaggtg
     8761 ctcaaggcca acaacgccaa atggctgaac gagcttgagg agatgatcgg gaagacgatc
     8821 ctggtgaaga gcgatccggc gttgccgccg gagcagtttg acatccaggg ttgacacccg
     8881 cggtgcagtt ggaggttgag ggctagaata gtcgggaacc cgggttcgct gcggcggacc
     8941 cggcagcctt taaggcgcct cgctccatcc gttgtacggc ccgaaattcc tgaaaatgca
     9001 tcctaaagtg aaacttggcg ttaatttccg tgtccaagta cctggtaggc ttttttgatt
     9061 cgtattccca acatcctgag gtgttctcaa tgatcgcgaa ctgtgatcgt caccgtgcgc
     9121 tgctgcggcg cgtggtgact accgcttttg ctgttgctgt cggggtctcg ctgaacgcgt
     9181 ctgcgcagac gtctttgccc gcggatcacg ccgcgaacgg ccagagttac tcgagctcca
     9241 acaattacca ggcatacctg agcaccgatt cgctgctcgg gggctcctcg tatgctgagc
     9301 cttcaggcgg cgccagtgct tctccgcaat acggcggcaa tcgtcgttcg tccaactatc
     9361 ccagctacga gagtcgctgg agccacttcg ctttcaatgc cggcgccggg tttacgatcc
     9421 ccatcggcaa caccaccagt ttcaatcgag cggaggccga agatccgaat ggccccctca
     9481 gccccagcca ggggcttggt tataacgtcg acctcggtgc cggctggaac ttcagcaagc
     9541 gcttcggcgt cctgctcgac tacgagttcc ttcgcgacag catccctaac gactacacag
     9601 acatcctcgc gggcggttcg ggtacgggac tgggcggcaa catcaatacg tggtcgctcg
     9661 cactcgatcc gatcatctac ctgcccatga gccgcaagag cggcgcctac gtcaccggag
     9721 gcgggggctt ttaccgcaag gttacgaact tcaccgagcc tgtcgaggaa tgcgactatt
     9781 actacggctg ctacggtgtt ccggtcaccg tcgagcactt ctccagcaac cagggcggtg
     9841 ccaacttcgg catcggtttc tatcacaaac tcttcgggga agacagcaac gggaaatttt
     9901 atgctcaggt gaagtacgtg ttcgtcaact ctccgcgccc gagcacctca aacttcggtc
     9961 aaggttccgg tactgaggag ctcctccccg tcaccttcgg cgttcgtttc tgaacgtaat
    10021 atgacgccag ttcccctttt gcgggggact ggcgtttttt gatccacagc catcctggtg
    10081 aacggcggcg tgcatatcga tttcagtgcg catgcgttcg cgccattgtg caggaactcg
    10141 atccaacggc gacgcaaggg atgtgtggca gggaacagcc tcgggagccg agcagttcga
    10201 cgtcgaccgg gttgcccagc ttgatttgga agccgacagg cttttggctg ctttttggcg
    10261 ataatctcca ggctgcgctc tgttctactt ccagctcaca gccaactgcc agcgcagatt
    10321 ccgcaccgca gtcttgcgca gcacgcgttc atactcctca agctcggaca ccacgcgggt
    10381 agtgtcggcg cccagagctg tcggcgcttc cttcagaaaa tcatgcaggg tgcagatcgt
    10441 gatcaaccca tcttcaggtg aaaaccattg cggaggaggc aaagtcctgg cccattccgg
    10501 atctccggct ccc
//
LOCUS       DASZYN010000664         5724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1062602, whole genome shotgun sequence.
ACCESSION   DASZYN010000664 DASZYN010000000
VERSION     DASZYN010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062602"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VHE33_17140"
     CDS             complement(<1..506)
                     /locus_tag="VHE33_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99234.1"
                     /translation="MRGDEELNRSRDAIDERIDLALRSYAEPPEFPDTRVMLARVMQS
                     ARDEETRRRGWWFWGAAVPAGLAVMATLVVMWTMRSPRHAEIAWTPKTPAVAESIVQR
                     LKPESSSGSRGAPQAVALKHGGRREAGLARTEERNPLPKQDVFPEPRPLSAQEEALVA
                     FASHVPAKV"
     gene            complement(493..1056)
                     /locus_tag="VHE33_17145"
     CDS             complement(493..1056)
                     /locus_tag="VHE33_17145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HVT99235.1"
                     /translation="MKGEEPRRVRPEDEAEFAGLVERHWKFAYRVAWAVLRNVHDAED
                     AAQETFVKIWRARAWRGLRDEKAYVARVSWRAAVDRVRESSSKRTPQRDHDRADAVIE
                     DVPDSALSPEGVVAAGDWEAAVHRLIDSLPEELRQVLALSVEMNSREIAEAIGIAEGT
                     VRTRLMRARAMLKEKMAAMEERRYARG"
     gene            1226..1477
                     /locus_tag="VHE33_17150"
     CDS             1226..1477
                     /locus_tag="VHE33_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99236.1"
                     /translation="MMDQHTMNMMMGMMSVIGVIALIGTAIYIIPFWFIFKKAGFSPW
                     LCLLLLLPVVNLVMLYVLAFTDWKVVPVAPAPIYVQPRA"
     gene            complement(1527..2594)
                     /locus_tag="VHE33_17155"
     CDS             complement(1527..2594)
                     /locus_tag="VHE33_17155"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99237.1"
                     /translation="MRYVSDAEPGIHRRKAGKGFRYVDADGKPVRDAETLKRIQSLVI
                     PPAWTDVWICANPRGHLQATGRDARGRKQSRYHPRWRAVRDETKYERMLLFGTALKGI
                     RARVEEDLAVPGLPRNKVLATIVRLMERTLIRVGNEEYARENGSYGLTTMRRKHVEIH
                     GSTVTFRFKGKSGKQHTVGVEDRRLARIVRQTQDLPGYELFEYLDDAGEPHKVDSADV
                     NDYLREISSEDFTAKDFRTWAGSVLASLALREFEEFESETQAKKNVVDAIKRVAQRLG
                     NTPAVCRKCYVHPAVLECYLEGKLERRMRRRAEELDDEALWAAEEEALRKEERALMDL
                     LRAQVERSASGRNGSKSMAAA"
     gene            2788..4068
                     /locus_tag="VHE33_17160"
     CDS             2788..4068
                     /locus_tag="VHE33_17160"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HVT99238.1"
                     /translation="MKRSALFPLLLLAAAVALTGCGSTSGNNNAGATGGSGGSGGSGG
                     SGGSGGTGGTGGSGGSSGSGSSGQTAANEFLYLNVGFADDSVYAWKIDTSTGALTPVA
                     GQPFPINIQNTSPCTVGCTFQLFTNPSGKYLVYTYGQQGGSGIGTAKVDASTGALTNV
                     TTNSSFSGYATVDPTGHFIYGNYSGDPTTRVNAIQAFSIGEDGSLTLVPGSPFSMGGY
                     GSYSAPAVSSNFVYQVSYNEQSSATSNTSITYGFSINPTTGALTKAGTTSDGMLGANQ
                     IVTPNGKFFYSEQDYNNNGTYDVQIVGYAINADGSLTPLSSAPQTTPDPGLAHMYMAP
                     NGNFLYHAGPAGLRAYAINPTTGALTLTATYSIPTSDAFAIDPTSSWVYAANQNNQMN
                     VYKADSTSGALTQVAADTTSAPDNPVNIVILRPQ"
     gene            complement(4091..4546)
                     /locus_tag="VHE33_17165"
     CDS             complement(4091..4546)
                     /locus_tag="VHE33_17165"
                     /inference="COORDINATES: protein motif:HMM:NF019238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HVT99239.1"
                     /translation="MPTLRNAADRKRLLDRLAEVTPASKPLWGRFDAPRMVCHLGDAL
                     DEALGRRVMPRLAPGVASAMLRRFPMKHLAIYVIPMPRNAKAPRELLADEPGDFEANR
                     RRVVESIEQLTKKPSGPAPEHFLFGSMTWERWNRLGWKHIDHHLRQFGN"
     gene            4759..5037
                     /locus_tag="VHE33_17170"
     CDS             4759..5037
                     /locus_tag="VHE33_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99240.1"
                     /translation="MGLEVGGTTDCPDWPKLGPSMLIATCLIVAIRTAKWAPDADPHL
                     AGRELGREIEYAAHVAHRVLSRLLRTDVTLFPQRKEPWYKPDGEDQPK"
BASE COUNT         1012 a         1917 c         1619 g         1176 t
ORIGIN      
        1 acctttgccg gcacatgact tgcgaacgcg accagtgcct cttcctgtgc gctcagtggt
       61 cgcggttccg gaaatacgtc ctgcttcggc aatggatttc gctcttctgt ccttgccagg
      121 cccgcctccc gtcttccgcc gtgtttgagg gccacagcct gaggggcgcc cctggatccg
      181 ctcgatgact caggcttcag tcgctgaacg atgctttccg ccaccgccgg cgtcttcggt
      241 gtccatgcaa tctccgcatg gcgtggactc cgcattgtcc acatcacgac cagcgtcgcc
      301 attacagcca gtcccgccgg cactgcagca ccccaaaacc accaaccccg tctccgcgtc
      361 tcctcatcgc gcgcactctg catcacccgc gccagcatga cacgcgtgtc cgggaattcc
      421 ggtggctccg cgtaactccg cagcgcgagg tcgatacgtt catcgatggc gtcccggctc
      481 cgattcaact cttcatcccc gcgcataccg ccgctcctcc atcgctgcca tcttttcttt
      541 cagcatcgct cgtgcccgca tcagccgcgt ccttaccgtt ccctcggcga tgccgatcgc
      601 ctcggcgatc tctcgtgagt tcatctcgac cgacaacgcc agcacctgcc gcaactcctc
      661 gggcaggctg tcgatcaggc gatgcaccgc cgcctcccaa tcccccgccg ccaccactcc
      721 ctcagggctc aaagccgaat ccggaacatc ctcgatgacc gcatctgccc ggtcgtggtc
      781 gcgttgcggc gtccgtttgg acgacgattc tcttacccga tccacggctg ctctccacga
      841 cacccgcgcg acatacgcct tctcatcccg caatccccgc caagcccgag ccctccagat
      901 cttcacgaag gtctcctgcg ccgcatcctc cgcgtcgtgg acattgcgca ataccgccca
      961 ggcgaccctg taggcgaact tccagtgccg ctcgaccaga cctgcgaact ccgcctcgtc
     1021 ctccggtcgc acccgccgtg gctcctcgcc cttcatccgc aaagacgccc ctgctctttc
     1081 aactgttcat ttatttgaaa atcgtccaca ccgccactgc gccaggacgc tgcaatctca
     1141 gcccgtaacc tgtacggggt cttgcctcgt ccccatctct ccgctaatct gacgccctgc
     1201 cgctatcttt cacccgaggt gttccatgat ggaccagcac acgatgaaca tgatgatggg
     1261 catgatgagc gtgatcggcg ttatcgccct catcggcacc gcgatctaca ttattccttt
     1321 ctggttcatc tttaagaagg ccggattctc tccgtggctt tgcctgctgc ttctgctgcc
     1381 cgtggtgaat ctcgtgatgc tgtacgtgct tgccttcacc gactggaagg tcgtgccggt
     1441 ggcgccggct ccgatctatg tccagccgcg agcctgacga ttggggagat cgccatcgct
     1501 cgactggata ccgtttggct cgcagctcag gctgcggcca ttgacttcga gccattgcga
     1561 ccggaagcgc ttctctccac ctgcgcccga agcagatcca tgagcgcgcg ctcctctttc
     1621 cgcaaggctt cttcttctgc ggcccacaag gcctcgtcgt ccagttcttc ggctctccgg
     1681 cgcatccggc gctccagctt gccttcgaga tagcactcca gcaccgccgg atgcacatag
     1741 catttgcggc agactgccgg cgtgttgccc agacgctgcg cgactcgctt gatcgcgtcc
     1801 accacgttct tctttgcctg ggtttccgat tcgaactcct cgaactcccg cagcgcgagc
     1861 gacgccagaa cgctcccggc ccacgttcga aaatccttgg ccgtaaaatc ctcgctgctg
     1921 atctcgcgca gatagtcatt cacgtccgcc gagtccactt tgtgcggctc gcctgcgtca
     1981 tccaggtact cgaacagctc gtatccgggc aagtcctgcg tttgccttac gatccgagcc
     2041 aggcgtcgat cttcgacgcc cacggtgtgc tgcttgccgc tcttcccttt gaagcggaac
     2101 gtcaccgtcg atccatgaat ctccacgtgt ttcctgcgca tcgtggtcaa gccgtaggaa
     2161 ccgttctcgc gcgcgtactc ttcattgccc acccggatca gcgtccgttc catgagacgc
     2221 acaatcgtcg ccaggacttt gttgcgtggc agtcctggca cggcgagatc ttcctctacg
     2281 cgtgcgcgaa tccccttcag ggcggttccg aagagcagca tccgctcgta cttggtctca
     2341 tcgcgcaccg cgcgccatcg cgggtgatat cggctctgtt tgcgtccccg tgcatcgcgg
     2401 cccgtagcct gcaaatggcc tcgcggattc gcacagatcc agacatcggt ccaggccggg
     2461 ggaatcacca gcgactgaat ccgcttcagc gtctctgcat cgcgaaccgg cttcccgtcg
     2521 gcatcgacgt agcgaaatcc cttgcccgct ttcctgcgat ggatcccggg ttccgcatcg
     2581 gaaacatatc gcaaccccgc ttctttcgct gacaacgcag gatctgtaac gaccactggc
     2641 actgtcgtct tcctgtgagc ccttttcata cgctgcgtga ggttggatgc acccagaccg
     2701 tgacgtcgac acgcgagctc tcccagggtt gcggccaact cgttcacctt tgcgccgcat
     2761 cctgccaagt gcaggtctga tttcctcatg aaacgcagtg ccctcttccc cttgctcctc
     2821 cttgctgcag cggtcgcact caccggctgt ggaagcacct ccggcaacaa caacgcaggc
     2881 gctaccggcg gatccggagg gtccggcggc agcggtgggt caggcggttc cggtggaacc
     2941 gggggaacgg gaggttccgg cggttcgagc ggcagcggca gttcgggaca aacggccgcc
     3001 aacgagttcc tctatctcaa cgtcggcttc gccgacgact cggtctacgc ctggaagatc
     3061 gacacatcga cgggcgcgct cacgcccgtg gctggccagc ccttccccat caacatccag
     3121 aacacgagtc cgtgcaccgt gggctgcacc ttccagctct tcactaatcc cagcggcaag
     3181 tacctcgtct acacgtatgg gcagcagggc ggatcgggca tcggaaccgc gaaggtcgat
     3241 gcgtccactg gcgcactcac caatgtcaca actaactcct ccttttccgg atacgccacc
     3301 gtcgatccta cgggccattt catctatggc aactactccg gtgatccgac gacgcgcgtc
     3361 aacgccatcc aggcgttcag catcggcgag gatggatcgc tcacactggt tccgggttcg
     3421 cccttctcca tgggcggcta tggcagctac tccgctcctg cggtctcgag caacttcgtc
     3481 taccaggtgt cctacaacga gcaaagcagc gccacgtcga acacatccat cacttacggc
     3541 ttctccatca atccgaccac gggtgcgctg accaaagccg gcacgacctc tgacggcatg
     3601 ctcggcgcca atcagatcgt gactcccaac ggcaagttct tctactccga gcaggactac
     3661 aacaacaacg gcacctacga cgtgcagatc gtggggtacg ccatcaacgc cgatgggtcg
     3721 ctgacgccgc tcagcagcgc gccgcagacg acgcccgacc cgggcctggc ccacatgtac
     3781 atggcgccca atggcaactt cctctaccac gcgggcccgg cgggtcttcg cgcgtacgct
     3841 atcaacccca cgaccggagc gctgacttta accgccacct actcgattcc gaccagtgac
     3901 gcctttgcga tcgatcccac gtcgtcctgg gtgtatgcgg ccaatcagaa caaccagatg
     3961 aacgtctaca aggccgacag caccagcggt gcgctgactc aggtggccgc tgataccacg
     4021 agtgcacccg acaatcccgt caacatcgtc attcttcgcc cacagtagct gtgggccttc
     4081 gagcgacagg ctagttgccg aactgccgga ggtggtgatc gatgtgcttc caaccgaggc
     4141 ggttccagcg ttcccaggtc atgctgccga agaggaagtg ttccggggcc ggaccggagg
     4201 gcttcttcgt gagctgctcg atgctctcca ccacgcgcct gcggttagcc tcgaagtcgc
     4261 ccggctcgtc ggcgaggagc tcgcgcgggg ccttcgcatt cctcggcatg gggatgacat
     4321 agatcgccag gtgcttcatg gggaagcggc ggagcattgc cgaggccacg ccgggagcga
     4381 ggcgcggcat gacgcggcgg cccagagctt cgtcgagggc atcgcccagg tgacacacca
     4441 tgcgcggggc gtcgaagcgg ccccagaggg gcttcgaggc gggagtgacc tccgccagcc
     4501 ggtcgaggag ccgcttgcga tccgctgcat tgcgaagggt gggcatgccg gcaatcttac
     4561 cgaaggtgga ggcaggaggt agccggcagg gctaacggat tccggatttg cgagggaggg
     4621 ggttgacccc ctccgaagtc attgatttca tgcggccgat ctcgcttcga cccccctcct
     4681 attaacgaag cagtacatcc atttctgcgg gttcaggttt cgcccgctgt ttcgcctttt
     4741 attcgctata ctgtccgcat gggactcgag gtaggcggca cgaccgattg tccggactgg
     4801 cccaagctgg ggccctcgat gctcatcgcg acctgtctga tcgtggcgat ccgaacggcg
     4861 aagtgggcgc cagacgccga tccccacctc gccggccgcg aactggggcg ggagatcgaa
     4921 tatgcggcgc acgtggccca tcgggtgctg tcgcggctgc tgcggaccga tgtgaccctg
     4981 ttcccgcaac gcaaagaacc ctggtacaag ccggacgggg aggaccagcc gaagtgaggg
     5041 cgggggcatg ccatggcaga gtgccgcgtt ttgtgtgcag tcgtgtggaa tttcgcattt
     5101 ccgcgagggc cttggcgcag gtaattgatt tcgccccact tgcttctgtt tactaacctg
     5161 acaaaagtca agtggttgat tccgggaaag atcgaaacgg ccgcatgggt tacagactcg
     5221 gccgttttgt tatagagacg gccgtttatg taacaaacgc ggccgtcttt gttatccaga
     5281 cggcggagtt tgtaacactc gtgggcgtgg gggtgacagg attcgacgca gaggcagtat
     5341 cccgaaaact ggccggctaa gtgcgggagg cgctcggata ccgaaggacg ggcgcgggtc
     5401 gggatcacga ctgcgatggg cacctggctc ctcctttcag acatacgagg tatgggcaaa
     5461 ggaaaacggc tcgccgcggc gagccgtttt tgggatattt ctccctattc tcaggatatc
     5521 aagtggagcg ggaaatcgtg ccaacgtttt ttggagttct gggcagataa gggttgcgga
     5581 atgagggaga acactttcgt accgtggcgc tggggcttga ccagagttta gctaaggtcc
     5641 ggtcagctaa gaagcggtcg gctaagcgcc ggttagctga ggagcggcca gctaaagagc
     5701 ggtcggcttg caatggccat cttc
//
LOCUS       DASZYN010000665         1685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1002503, whole genome shotgun sequence.
ACCESSION   DASZYN010000665 DASZYN010000000
VERSION     DASZYN010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002503"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(640..1332)
                     /locus_tag="VHE33_17175"
     CDS             complement(640..1332)
                     /locus_tag="VHE33_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99241.1"
                     /translation="MRRISFLAALMAIAAVSPNHAQNSGPANSGGAVPPGYQVYTNAE
                     LHLTLTYSAELTGVDGAFATTAARRMIYGDEQLDAAREDACVKVLLAVGKGSEGKGSW
                     ARVGAVEIRGECFPPKVLQKRKDTQMLLRNLVRQGTTLMGMMPLEQPGGYQIEGHWAS
                     FGAAQGEPVTKSDIQSGEAQLIGVGAIAVQGWILGWVVETNDAAMFNQLLGSGVVFGT
                     GTPQRLFGGGVQ"
     gene            complement(1359..>1685)
                     /locus_tag="VHE33_17180"
     CDS             complement(1359..>1685)
                     /locus_tag="VHE33_17180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HVT99242.1"
                     /translation="CLREYDSIGRYGGEEFLILIPGYDPAEGRSRLDELVRSVHDHRF
                     LNDGHEIQASCSFGVTVFRPEERLASIEELLATADAALYRAKAAGRNCAIFAELTELQ
                     GFVGRA"
BASE COUNT          309 a          642 c          406 g          328 t
ORIGIN      
        1 cgccatcggc accggctatc tgatcgcaag ccagctcttc ggcgtcagcc cctggaatcc
       61 cctgcttctt gccggcgcca ctgcattgtt gggcctggct gcgctggtgg ccgcgctgat
      121 tcccgcccaa agagccgcga gtatcgatcc catacaggct ttgcgaagcg aataacgaat
      181 ctcagacctt cgtaccgtcc tcggctccga tttaccttgc attgctcctc ggaaaatccc
      241 acactgagcc tacgggaagc cctgtccact cgacagggct tttcctttgg cccaagccga
      301 tcacaatgcg ctctttgaca tttggcggca gccgcccgaa agataattgg aaccgtctgc
      361 ggaacagaac cgcacgccgt tggtcgatct cagaaccccg gccgtgtcat gcacgcagcc
      421 gcttctgtta cacaagcggc cgtgtatatg acacaagcgg tcgtgcgtat gacacaagcg
      481 gccgtctttg ttacaccaac agccgcttcg aagtttcctg ttttcaggag gttagcatgt
      541 tgtcgaactt gccatccccg acgaagcccc gaaaactcaa ttagatacct caacagggcc
      601 tcggaagaac ccgacttcca gtccggcgct cgaatcgccc tattgcaccc cgcccccaaa
      661 cagcctctgc ggcgtccccg tcccgaaaac cacaccgctt cccagcagct gattgaacat
      721 cgccgcgtca ttcgtctcaa ccacccagcc gaggatccac ccctgtaccg cgatcgctcc
      781 cactccgatc agctgcgcct ccccgctctg gatatcgctc ttcgttaccg gctctccctg
      841 cgcagcccca aaactcgccc agtgcccctc gatctgatat ccccccggct gctccagcgg
      901 catcattccc atcaacgtcg ttccctgccg caccaggttc cgcaacagca tctgcgtgtc
      961 cttccgcttc tgcaacacct tcggcggaaa gcattcgccc ctgatctcaa ccgcgcccac
     1021 cctggcccac gagcctttcc cttcgctgcc cttccccacc gccagcagga ccttcacgca
     1081 cgcgtcctcc ctggccgcgt ccaactgctc gtcgccgtag atcattcgcc gcgccgcagt
     1141 cgtggcaaac gccccatcga cccccgtcag ctccgcggaa taggtcaaag tcaggtgcag
     1201 ttccgcgttc gtgtagacct ggtacccagg tggcacagcc ccgccgctgt tcgccggtcc
     1261 gctgttctgt gcatggttcg gcgacaccgc cgcgattgcc atcagcgccg caaggaacga
     1321 gattcgcctc atggccgcca gcgtaccacc cgccggcgct acgccctgcc cacgaacccc
     1381 tgcagctcgg tcaactccgc aaagatcgcg cagtttcgcc ccgccgcctt cgcgcggtag
     1441 agcgccgcat ccgccgtggc cagcagttcc tcaatggaag ccagccgctc ctccggacga
     1501 aacaccgtca ccccgaagct gcagctggct tgtatttcat ggccatcgtt cagaaaacgg
     1561 tgatcgtgaa cgctccgcac cagctcgtcc agacgactcc gcccttccgc cggatcgtat
     1621 cccggaatca ggatcaggaa ctcctcgccc ccgtaccgtc cgatgctgtc gtactcgcgc
     1681 aggca
//
LOCUS       DASZYN010000666         3100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_230172, whole genome shotgun sequence.
ACCESSION   DASZYN010000666 DASZYN010000000
VERSION     DASZYN010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230172"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1531
                     /locus_tag="VHE33_17185"
     CDS             <1..1531
                     /locus_tag="VHE33_17185"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HVT99243.1"
                     /translation="LAERLISDLDRPKPEVVLDVAVLEVSRDVLRNIGIQLPQTASIN
                     FQPSNANLNNTNNNNGNGTDNGNNNNGGNNNSSSITLNSLAHLNSTNFAVTIGQAAVN
                     LLLTDTRSRIIQNPQIRASDGQEATLKIGERIPIATGSYQTGAATAIVSSLVNTQFQY
                     IDVGVNMDIKPTVHFDRDVSMKFKVEISATNGNSNIGGITEPIITQRVIDQTIRLKDG
                     EPNLLGGIVQHQTTLTVSGTPGLAELPLLKYFFSTQQKQTTDDEIVFLVVPHVVRAVQ
                     VGAENLQAIDTGTTNNVQLRRINSAGVEVADTNPPAAPAQAVHTQNLPPAVAPVQESA
                     PAQQGAPPGPPPSAAAAAQQAMTNMEQQQASGPPVNLQLSPPQSTEKVGSTFKVFVDL
                     SGGNDVFGAPMNVQYDPTKLTLINVDSDDPQHPNVLGRDGQAVALVHRDDGQGNVAIA
                     ASRPPGTKGVTGSAGTLCVLTFQAKAAGDASLAVTRPVVRNSDQQPIRATGSQAVVHI
                     Q"
     gene            1551..2033
                     /locus_tag="VHE33_17190"
     CDS             1551..2033
                     /locus_tag="VHE33_17190"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HVT99244.1"
                     /translation="MQLMGFRTGKVSGDREAGLTLVELIVTVAILGILASAAVPIARF
                     QVKREKERILRYDLWQMRRAIDAYKDAADKHAFQTKIDSQNYPPDLQTLVDGVDVQGK
                     KVRFLRHIPIDPMTGNDDWGLRSMQDDPDSDSWSGNNVFDVYSKSDGTALDGTKYKDW
                     "
     gene            2037..2447
                     /locus_tag="VHE33_17195"
     CDS             2037..2447
                     /locus_tag="VHE33_17195"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HVT99245.1"
                     /translation="MRLKTPIRRSEEGFTLVELMVVMLIIGILAAVAIPSFVAAIKSA
                     KESALKEDLHVMRDAIDSYTMDKNKAPQSLDDLVQSGYLKEIPQDPFTHSRDTWVTAS
                     DDTYADLDQTEPGINNVHSGSQENGSDGQPYSSW"
     gene            2838..>3100
                     /locus_tag="VHE33_17200"
     CDS             2838..>3100
                     /locus_tag="VHE33_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99246.1"
                     /translation="MSVASMALPELTNIHESFLTLSLMARERFGGSLGGRLLLRQEFN
                     AEGAAVVIAASVAGAASLCVDGDAERLREGLRGGHVDFVVATL"
BASE COUNT          685 a          926 c          947 g          542 t
ORIGIN      
        1 gctggcggag agactgatca gcgatctgga tcggccgaag ccggaagtgg tgctggatgt
       61 ggcagtgctg gaagtgagcc gcgatgtgct gcgcaatatc ggcattcagc tgccgcagac
      121 ggcgagcatc aacttccagc cttcgaacgc gaacctgaac aacaccaaca ataacaacgg
      181 caacggcacc gataacggca acaacaacaa cggcggcaac aacaattcga gcagcatcac
      241 gctgaacagc ctcgctcacc tgaactcgac caacttcgcg gtcaccatcg gccaggcagc
      301 ggtgaatctg ctgctcaccg atacgcggtc gcgcatcatt cagaatccgc agattcgcgc
      361 ctccgacgga caggaagcga cgctcaagat cggcgaacgc atcccgatcg ccaccgggtc
      421 gtatcagacc ggcgcggcca ccgcgattgt cagctcgctg gtcaacacgc agttccagta
      481 catcgacgtt ggcgtgaata tggacatcaa gccgacggtt cactttgacc gcgacgtcag
      541 catgaagttc aaggtggaaa tctcggctac caacggaaat tccaacatcg gcggcatcac
      601 cgagcccatc atcacgcagc gcgtgatcga tcagacgatt cgcctgaagg atggcgagcc
      661 gaacctgctg ggcggcattg tccagcacca gacaacgctg accgtgagcg ggacaccggg
      721 actggccgag ctgccgctgc tcaaatactt cttcagcacc cagcagaagc agacgaccga
      781 cgacgaaatc gtgttcctgg tggttccgca cgtggtgcgc gccgtgcagg tgggcgccga
      841 gaacctgcag gcgatcgata ccggcacgac gaacaacgtt cagctgcgac gcatcaattc
      901 tgcaggcgtg gaggttgccg acacgaatcc gccggccgcg cccgctcagg ctgtgcacac
      961 acagaatctt ccgcccgccg tcgctccagt gcaggagtcc gctccggccc agcagggggc
     1021 tccgcccgga ccgcctcctt cggctgcggc agcggcacaa caggcgatga cgaacatgga
     1081 gcagcagcag gcaagcggac cgccggtcaa cctgcagctg agcccgccac agtcgaccga
     1141 gaaggtggga tccacgttca aggttttcgt cgatttgtcc gggggcaacg acgtcttcgg
     1201 cgcgccgatg aatgtgcagt acgatccaac caagctgacg ctgatcaatg tcgactccga
     1261 cgatccgcag cacccgaatg tactcggcag ggacggccag gcggtggctc ttgtccatcg
     1321 cgacgatgga cagggcaacg ttgccattgc agcttcgcga ccaccgggga ccaagggtgt
     1381 aactgggtcg gccggcacgc tgtgcgttct cactttccag gcgaaggcgg ccggcgacgc
     1441 ttcgctggca gtcacacggc ctgtggtgcg caacagcgac cagcaaccga tcagggcaac
     1501 gggatcgcag gcagtggttc acatccagtg atcgtgggca tgagtgacgc atgcagttga
     1561 tggggttcag aacggggaaa gtcagcgggg atcgggaagc cggcctgacg ctggtggagc
     1621 tgattgtcac ggtggccatc ctcggtatcc tggcgtccgc tgccgtgccg atcgcgcgct
     1681 tccaggtgaa gcgcgagaaa gaacgcatcc tccgctacga tttgtggcag atgcgtcggg
     1741 ccatcgacgc gtacaaggac gcggccgata agcatgcttt ccagacgaag atcgacagcc
     1801 agaattaccc gccggatctg cagacgctgg tggatggggt cgacgtgcag gggaagaaag
     1861 tgcggtttct gcgtcacatc cccatcgatc cgatgacggg caacgatgac tggggactgc
     1921 gatcgatgca ggacgatccc gacagcgact cctggagcgg aaataatgtc ttcgatgtgt
     1981 actcgaaatc cgacggcacc gcgctggatg gaaccaagta caaggactgg taaccgttgc
     2041 gcctgaaaac tccaattcgc agatcggaag aaggcttcac gctggtggag ttgatggtgg
     2101 tgatgctgat catcggcatc ctggcggcgg tcgccattcc cagctttgtt gccgcgatta
     2161 agagcgccaa agagtcggcg ctgaaggaag acctgcacgt gatgcgcgac gccatcgact
     2221 cctacaccat ggacaagaac aaagcgccgc agtccctcga cgatctggtg cagagcggct
     2281 acctgaaaga gattccgcag gatcctttca ctcacagccg cgacacctgg gtgacggcaa
     2341 gcgacgacac ctacgccgat ctggaccaga cggagccggg catcaacaac gtgcacagcg
     2401 gatcgcagga aaacggatcc gacggccagc cgtactcgag ttggtgaagg aacggccgat
     2461 atataatgaa gtagttcccg gaagggtcga atcctcccgg gaagttggct actgcaccag
     2521 cagcagcgtg agcacaagga cagcggccgc cagcacaaag cgccaatcca gcgcaatcgt
     2581 gatggcgata cgagtccagg atgatttctt attcatccct ctggactcac cccctttcat
     2641 tcgaacggcc cgcaggtcgt cagcggcctg cgagccgtcg gtccgatctt ccggaccgat
     2701 cctgcgctaa ccgcattcgc actttgaaag aggcactccc agtttaggga gtgttgtgcg
     2761 caccctggtc actttcacct tgttgaggaa aacccaggta atgatccgca gggcttgcat
     2821 ttctgcaaca tgacagaatg tcagttgcga gtatggcgct gcctgaactc accaacatcc
     2881 acgaatcctt tcttacgctc agcttgatgg ctcgagaacg ctttggcggg tctctcggcg
     2941 gacggcttct tcttcggcag gaattcaatg cagaaggcgc ggccgtagtg atcgccgcca
     3001 gcgttgccgg ggctgcttcg ctctgtgttg acggcgatgc ggaacgcctg cgcgagggtc
     3061 tgcgcggagg ccatgtggac tttgtggttg cgacgctcga
//
LOCUS       DASZYN010000667         8322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_166797, whole genome shotgun sequence.
ACCESSION   DASZYN010000667 DASZYN010000000
VERSION     DASZYN010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8322
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166797"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VHE33_17205"
     CDS             complement(<1..1230)
                     /locus_tag="VHE33_17205"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HVT99247.1"
                     /translation="MSEGDERSGGSESRREFLIRAATAFAAGGVSAVFPLACGQQRSK
                     AGAIAPAPTLNDGSGARSLRAHGAARGLLVGCAVNPDHLDGDAEYARIVAEQASIVVP
                     ENAMKWAALRPSPTQYDFRQADDVVAFALSHEQKVRGHNLCWHEALPAWFAATVTKAN
                     AARYLTQHIRTVAGRYAGKLHSWDVVNEAVEVKDGRPDGLRKSPWLELMGPSYLELAF
                     RTAREADRAALLTYNDYGIELDTPEQIDKRGQVMMLVRRLQARGVPIDAVGIQSHLSA
                     GDAPGDGLIHFIRELRAMNLQVFITEMDVNDRKLPEAVAERDAGVAKTYRDFLTAVLA
                     EPNVTAALTWGITDRYTWLNGLQHASRADGKPERPLPFDYDYNPTPAFFAERDAIDAR
                     PARRLPGADPYAPFRPGR"
     gene            complement(1227..1478)
                     /locus_tag="VHE33_17210"
     CDS             complement(1227..1478)
                     /locus_tag="VHE33_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99248.1"
                     /translation="MFGSLRFIWNATSGHRLRPWRSEYLKWRIETYSGMKADELRARD
                     IVAFAWREKWNLLRFLRWTDRMEAWRKAGAGHRSGTGES"
     gene            complement(1666..2598)
                     /locus_tag="VHE33_17215"
     CDS             complement(1666..2598)
                     /locus_tag="VHE33_17215"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HVT99249.1"
                     /translation="MNWAHRGNYSAAISSLKKAEKQDGGHCKECAYKIIDYGLKLDDF
                     KAADAAAQDLISAAQTPKEVAEAHLERATVQLREAVEKNKMDCFAEADREFEATLAAW
                     SNVPTACFYDGVSLAHLNRDADAKARFAQYLEIAPKDAADRERAERFIENIDLARARL
                     APAFAITTLDGRQVSLDRLAGKVVLIDFWATWCGPCRVSLPKIRDIAEKFSDEPLVVL
                     SISLDSNEAKWKDFVAENHMSWPQYRDGGFDGPVSRLFSVNSIPHTFTIDADGILQEE
                     HVGSHADFQGRLKKLLARAHQRQEALQVAPKPRS"
     gene            complement(2809..3813)
                     /gene="murQ"
                     /locus_tag="VHE33_17220"
     CDS             complement(2809..3813)
                     /gene="murQ"
                     /locus_tag="VHE33_17220"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF009222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HVT99250.1"
                     /translation="MGTTLNPHTDAGEGGKVHSNGSNGSNGTNGTPANIQDLLTEKAN
                     EATRGLDTRSAVEIARIINQEDQKVAAAVKKAIPEIAHVIDLVAHSLREGGRLIYVGA
                     GSSGRLAALDACEIPAYFSTSPQTVQYIMAGGPKALASPVEVNEDSEDLGKRDIARRR
                     PSRKDVVIGLSASGRTPYVVAAIAYARTLGARTACITCNPNTPLSNAAETAIVAEVGP
                     EVISGSTRLKAATAQKMITNMITTGAMTRLGYVYENLMVNVHMHNSKLIERGIGILMR
                     ACEVGREKAVDVIKSAGRSVPVALVMLKANVDKPEAVRRLARAEGNVRRAIEDSTMDV
                     "
     gene            complement(3959..4663)
                     /locus_tag="VHE33_17225"
     CDS             complement(3959..4663)
                     /locus_tag="VHE33_17225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT99251.1"
                     /translation="MIRAEHLGKVYRSGRLEVPALRDATFSVEAGEFVSIVGPSGSGK
                     STLFYLLGGLTRATTGKVFIGGVDFSTLDDGERTRMRRARIGFIFQKFNLLPTLSARG
                     NIEIAYEIAGRTEPMDQKYLEHIAGLLNIAGRLQHRPSELSGGEQQRVAIARALITRP
                     SIVLADEPTGNLDTENSDAVLEMLRRSNQELKQTVLMITHNPEAAAIGDRIIHMRDGK
                     IRGIETGQKAVGRHEW"
     gene            complement(4772..5218)
                     /locus_tag="VHE33_17230"
     CDS             complement(4772..5218)
                     /locus_tag="VHE33_17230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT99252.1"
                     /translation="MRPKKTILCVDDNEQALAVRKFLLETRGGYRVVAALSGRDAIEA
                     FRRGGVDLVLSDLVMPNMNGNEMVRRMKEMAPEIPMILISGSVKTFDHTNRADAFLPK
                     GSSSPVEMLERIRQMIARKRGPKKHLRPMYETADAAFDALEHAVAS"
     gene            complement(5791..6240)
                     /locus_tag="VHE33_17235"
     CDS             complement(5791..6240)
                     /locus_tag="VHE33_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99253.1"
                     /translation="MRRVAVLSALLVSASLCWAHGLNDRPPDARELADLEAKAASASP
                     NEQPYIYAELVHSMTEMATAEYKAGDDVQASAFLKRAQGYAGKIQMSLLRDAKKLKNA
                     EILIRHTAFRLKELMTGASLDDRPTLEATIQQLNQVQSDMMTQIFRH"
     gene            complement(6397..7773)
                     /locus_tag="VHE33_17240"
     CDS             complement(6397..7773)
                     /locus_tag="VHE33_17240"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT99254.1"
                     /translation="MSHKAQSAGHRAQERLSDFVGRAGGVLLGLVLLAGLGRAAWAQP
                     VPLPPGTTGDDQPTQASSPLDEVESAIEAKKYALATARLEIYLSAHPTDARALFDRGY
                     VEDAQGHTDAAEGWYRKAVAADPKQFEARSALGLLLAGKGNARGNAEAIEQLQAAAQL
                     EPNPPNPTAKAQVDRALARLLHKSDPTGARDALVEALKLTPETPSDTLLAGQIAEAAG
                     DLEIAEQEYRQALRNQPDLSDASAGLAHLLIAEKRYADAQPVVEAALRRDPQNGALSA
                     QLAAILNGEGKTGDALAVLEKLHHSEPDNAAITSMLADADFQAGKLAEADALYRQLLV
                     KNPDDPDLLDAHGQILVRQKQYQEAIGAFRKAIAARADDIDAWSGIAFADSELHEYQD
                     ELTALSMRSKYAPDSAASLFLWATAYDNLHQSKAAADYYHRFLAAAQGKFPDQEWQAK
                     HRLVALEK"
     gene            complement(7770..>8322)
                     /locus_tag="VHE33_17245"
     CDS             complement(7770..>8322)
                     /locus_tag="VHE33_17245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99255.1"
                     /translation="LQMTVREALRFFAGHPRIVDKLAVLEEIGLGYVRLGQSATTLSG
                     GEAQRVKLAAHLASTRSESRGPDGKKAGVRSRVLYILDEPTTGLHFDDVGKLLTAFRK
                     LIDSGGTLVVIEHNLDIIKWADWVIDLGPEGGAAGGQIVATGTPEEITRNELSHTGQW
                     LARALAGHGGVEPVHAVESEVFQ"
BASE COUNT         1436 a         2970 c         2263 g         1653 t
ORIGIN      
        1 ccgcccaggc ctgaacggcg cataaggatc agcccccggc agcctccgcg ccggtcgagc
       61 gtcgatcgcg tcgcgctcgg caaagaacgc cggcgtcgga ttgtagtcgt aatcgaacgg
      121 cagcggccgc tccggcttcc catcggcccg cgacgcatgc tgcaacccgt tcagccacgt
      181 gtagcgatcc gtgatccccc acgtcagcgc tgccgtcaca ttcggctccg ccagcaccgc
      241 cgttagaaaa tcccgatacg tcttcgccac gcccgcatcg cgctccgcca ctgcctccgg
      301 cagcttgcgg tcgttcacgt ccatctccgt gataaaaacc tgcaggttca tcgcccgcag
      361 ctcgcgaatg aaatggatca gcccatcccc cggcgcatct ccggcactca aatggctctg
      421 gatccccacc gcatcgatcg gcacgccgcg cgcctgcaga cgccgcacca gcatcatcac
      481 ctgcccgcgc ttgtcgatct gctccggcgt gtccagctcg atgccgtaat cgttgtacgt
      541 cagcagcgcc gcacgatcgg cctcgcgcgc ggtccgaaac gccagctcca gatacgacgg
      601 tcccatcaat tccagccacg gcgacttgcg cagcccatcc ggccgcccat ccttcacctc
      661 aaccgcctcg ttcaccacgt cccatgagtg cagcttcccc gcataacgcc cggcaacggt
      721 ccggatatgc tgcgtcagat accgcgcggc attcgccttc gtcaccgtcg ccgcaaacca
      781 cgcaggaagc gcctcgtgcc agcacagatt gtgcccgcgc accttctgct catgcgacag
      841 cgcgaaagcc accacatcgt ccgcctggcg aaagtcatac tgcgtgggac tgggccgcag
      901 cgccgcccac ttcatcgcat tttccggaac cacgatgctg gcctgctcag ccacgatccg
      961 cgcatactcc gcatcgccgt ccagatggtc cggattcacc gcgcatccca ccagcagtcc
     1021 tcgtgcagcg ccatgggcgc gcagcgaccg agccccgctc ccatcgttga gcgtcggcgc
     1081 gggagcgatg gctcctgctt tcgatctctg ctgcccacaa gcaagcggaa aaaccgccga
     1141 caccccaccg gccgcaaacg cggtcgcggc gcggatcaga aattcccgcc ggctttctga
     1201 cccgcccgag cgttcatcgc cttcgctcat gactccccgg tccctgatcg atgccccgct
     1261 ccggccttcc tccacgcttc catccgatcg gtccagcgca ggaagcgcag cagattccac
     1321 ttctcccgcc acgcaaacgc aacgatgtcg cgcgcgcgca gttcatccgc tttcatgccg
     1381 gaataggttt cgatccgcca cttgagatat tcgctgcgcc agggccgcag ccgatgcccg
     1441 ctggtcgcat tccagatgaa tcgcagagat ccaaacaaac gagtcaatcc tcccgccgaa
     1501 tcgcacgaat cccgcgctga ccgcagccgc tgcgttggct tgttgggtga tcctgcgctg
     1561 agtatagctg gagccttgcc gcactcagga ccgaagagct gcacccttgt cggccccgcg
     1621 ccgctcataa gcttatgacc gatgtcgggg ccgccacggc tccgatcatg atcgaggttt
     1681 cggtgcgacc tgcagcgctt cctgccgctg atgggcgcgg gccagcagtt tctttagcct
     1741 cccctgaaaa tcggcgtggc tgcccacgtg ttcttcctgc aggattccgt cggcatcgat
     1801 cgtaaacgtg tgcggaatgg aattgacact gaagaggcgc gagacaggac cgtcgaatcc
     1861 gccatcgcga tactgcggcc agctcatgtg gttctctgcc acaaaatcct tccatttcgc
     1921 ttcgttcgaa tcgaggctga tgctgagaac caccagcggc tcatcagaga acttttctgc
     1981 gatgtcgcga atcttcggca gcgatacccg gcagggaccg caccaggtag cccagaagtc
     2041 gatgagcacc accttcccgg ccagcctgtc gagagaaacc tggcggccgt ccagagtcgt
     2101 gatggcaaac gccggcgcca ggcgggcgcg tgccagatca atgttttcga tgaaacgctc
     2161 cgcccgctca cggtcggcag catctttcgg agcaatctcc aggtattgtg cgaaccgggc
     2221 tttagcatcc gcgtcgcggt tcaggtgcgc cagcgagacg ccatcataaa agcaggccgt
     2281 gggcacattg gaccaggctg ccagtgttgc ctcaaattcg cggtcggcct cggcaaagca
     2341 gtccatcttg ttcttctcca ccgcctcacg caactgcacg gtggcgcgct caagatgcgc
     2401 ctcggcaacc tctttcggag tttgagcggc gctaatcagg tcctgcgcgg cggcgtcggc
     2461 ggctttgaaa tcatccaact tcaggccata gtcgatgatc ttgtatgcac attctttgca
     2521 gtgtccgccg tcctgcttct cagccttctt cagggaggag atcgccgcgc tgtagttacc
     2581 tcgatgcgcc cagttcatcg cctcctgata ggtctttcgg gcccgggcgt cggtcgggcc
     2641 tgcggtatcc tgggccctgg tgattgtgag agaaaaaaga aaagcactca aaacgacagc
     2701 gggacgaagc atcgaacaac ttcctcgcga tagcaccgta tcacggagcc atgcgcagga
     2761 aaataccgct ttgaaaatga gtttaagtcg ggatgaagag gtccctgctc agacgtccat
     2821 cgtcgagtct tcgatcgccc ggcgaacatt gccctcggcc cgtgccagcc gccgcaccgc
     2881 ttccggctta tcgacattcg ccttcagcat caccagcgcc acaggcacgc tccggcccgc
     2941 ggacttgatc acatccaccg ctttctcccg gcccacctcg caggcgcgca tcaggatgcc
     3001 gatgccccgc tcgatcagct tcgagttgtg catgtgcacg ttcaccatca ggttctcgta
     3061 cacatacccc agccgcgtca tcgcgccggt cgtgatcatg ttcgtgatca tcttctgcgc
     3121 cgtcgccgct ttcagccgcg tcgaaccgga aataacctcg ggaccgactt cagccacaat
     3181 cgccgtctcg gctgcgttgc tcagcggtgt gtttggattg cacgtgatgc aggccgtcct
     3241 ggctcccagc gtccgcgcat acgctatcgc cgctacgaca tacggtgtcc gtccgctggc
     3301 cgaaagcccg atcaccacat ccttgcggct cggccgccgg cgcgcgatgt cgcgcttgcc
     3361 cagatcttcc gaatcttcgt tgacctcgac cggcgaagcc agcgccttcg ggccgcccgc
     3421 catgatgtac tgcaccgtct gcgggctggt cgaaaaataa gccggaatct cacaagcatc
     3481 cagcgcagcc agacgcccgc tcgagcccgc tccgacatag atcagccgtc cgccctcacg
     3541 caaactgtgc gccaccagat cgatgacgtg cgcgatctcc gggatcgcct tcttgaccgc
     3601 cgccgcaacc ttctggtctt cctgattgat gatccgggcg atctccactg cggatcgcgt
     3661 atccagcccg cgggtcgctt cgttcgcctt ctccgtcagc aagtcctgga tattggctgg
     3721 cgtcccattg gttccgttcg agccgtttga gccgttcgaa tggaccttcc cgccttcccc
     3781 ggcgtcggta tgtggattga gtgtcgttcc cattttttcc gggttttggc cgtacatgcc
     3841 ctccataatt ctatcgcgtt atggcatgca cgcggcgtca atattgtccg acagatgtgt
     3901 tagttgtttg tcactaattt gtcatggcct tccatgctgt cgaatctcta tcggggtcct
     3961 accattcgtg ccggccgacg gccttttgcc ccgtctcgat gcctcggatc ttcccgtccc
     4021 gcatatgaat gatccggtct ccgatcgccg cagcctccgg attgtgcgtg atcatcagca
     4081 ccgtctgctt cagctcctgg ttcgaccgcc gcagcatctc cagcacggcg tcggaattct
     4141 ccgtgtccag gttccctgtc ggctcgtccg ccagcacgat cgacggccgc gtgatcagcg
     4201 cgcgcgcaat tgccaccctc tgctgctctc cgcccgacag ttccgacggg cgatgctgca
     4261 gccgtccggc gatattcagc aggcccgcaa tgtgctccag atacttctgg tccatcggct
     4321 ccgtgcgccc ggcaatctcg tacgcgatct cgatatttcc ccgggctgaa agcgtcggca
     4381 gcagattgaa cttctgaaag ataaatccaa tccgcgcgcg ccgcatgcgc gtgcgttccc
     4441 catcgtccag cgtcgaaaaa tcgacgccgc caataaacac tttgccggtt gtcgctcgcg
     4501 tcagtccgcc cagcaggtaa aacagcgtcg atttcccgct gcccgaaggt cctacgatcg
     4561 agacaaactc ccccgcctcg accgaaaacg tcgcgtcgcg aagcgccgga acttcaagcc
     4621 tgccggatcg gtagactttg ccgaggtgct cggcgcgaat catcgattcc acgtgtgcga
     4681 ttgtaaatgg gccggtggaa gttccggccc actctggagg agatgccgtc tcagcgatca
     4741 ggggtgcccg cggcgcaggt ccgcctgtgg gtcacgacgc cactgcgtgc tccagcgcat
     4801 cgaacgccgc gtcggccgtc tcatacatcg gccgcagatg cttcttcgga ccgcgcttgc
     4861 gcgcgatcat ctggcgaatc ctctccagca tctcgaccgg cgaggatgag cctttgggca
     4921 gaaatgcgtc cgcccgattc gtgtgatcga aggtcttcac gctgccgctg atcaggatca
     4981 tggggatttc cggcgccatc tctttcatgc gccgtaccat ctcgttgcca ttcatattcg
     5041 gcatcaccag gtcgctcagg accaggtcga cacccccgcg gcgaaatgcc tcaatcgcat
     5101 cgcgcccgct cagagctgcc acgacgcgat aaccgccgcg ggtttcaaga aggaatttgc
     5161 gaacagcaag cgcttgttcg ttatcgtcaa cgcagagaat cgtctttttt ggtctcattc
     5221 ttcttccctg gcaactctcg ccctggtgcg agaggactgc ggcgaggcgc tcagcatggt
     5281 cggcgcgccc gcctgcgtgg aggtggccgc acccggcttc cggcttcctg cgcccgcgct
     5341 cggcttgccc aaaaagcgcg cgacgctgcg tgcccgccgg aacgcgggat gcgggggaac
     5401 agtaccgccg gctgcggctg gctctctgcg cgcccttccc cttccgtgct tccggatcgt
     5461 gggatgcggc gggggccgtg tgccttgcgt tacggttcca gttcagtttt cagcgaatgc
     5521 agtctcgttc ctttcgaaac gccacgtcag agtacggagc actgccggtc accgcaatag
     5581 ggtaaaaaaa gcgaaaagac gccgccttcg taatcgcact ttgcacgggg ttctgttatc
     5641 gcccgatgaa tattggctgg gacgccgccc tgggctgtgg aacgtgtgtg gatcacgcgg
     5701 ggaaaagccg gacacttgca gcgattgcgg tgcggctcat gaataaactt cgtcccgata
     5761 cgagcgtccc gctcaacctg gattctcgtt tcagtgacgg aagatctgcg tcatcatgtc
     5821 cgactgcacc tggttcagct gctgaatcgt cgcctccagc gtcgggcgat cgtccagcga
     5881 cgcccccgtc atcagctcct tgagccgaaa cgccgtgtgg cggatcagaa tctccgcatt
     5941 cttcagcttc ttggcgtcgc gcagcaggct catctgaatc ttgcccgcat acccctgggc
     6001 ccgcttcaga aatgccgacg cttgcacgtc gtccccagcc ttgtactccg cggtggccat
     6061 ctcggtcatc gagtgcacca gctcggcata gatatacggt tgctcgttgg gagacgcact
     6121 ggcggctttc gcttccagat cggccagctc ccgcgcgtca ggcgggcggt cgttcagccc
     6181 atgcgcccag cacagagacg cagagacgag taaagcagag aggactgcga cgcgccgcat
     6241 cgcgcacctc cggggtcgtg cgttccaaga acaataccgc agttttgtgc cgttgcgcta
     6301 ccacatctgt gtcagccccg aacacagcca atcaaaggta acgccgtccg ttgcccgttg
     6361 agtaccgtcg gaacccaaac ccacgctcaa gcaccgctac ttttcaagag cgaccagccg
     6421 gtgcttcgcc tgccactcct gatcggggaa ttttccctga gcggcggcaa ggaaacgatg
     6481 ataataatcc gccgctgcct tgctctgatg aagattatcg tacgcagttg cccacagaaa
     6541 aagacttgct gcactgtccg gcgcatactt tgatcgcatg gaaagcgccg tcagttcgtc
     6601 ctggtattcg tgcagctccg agtccgcaaa cgcgatcccc gaccacgcat cgatgtcgtc
     6661 ggcccgcgcc gcaattgcct tgcggaaggc tccaatggcc tcctgatact gcttctgccg
     6721 caccaggatc tgcccgtgcg catccagcaa atccggatcg tcggggtttt tcaccagcag
     6781 ttgccggtac agcgcatccg cctctgccag cttccccgcc tgaaaatcgg cgtccgccag
     6841 catcgacgtg atcgccgcgt tgtcaggctc gctatggtgc agcttctcca gcaccgccag
     6901 cgcatccccg gtcttcccct ccccgttcag gatcgccgcc agctgcgcgc tcaaagcccc
     6961 gttctgcgga tcacgccgca gcgccgcctc caccactggc tgcgcatctg catagcgctt
     7021 ctccgcaatc agcaggtgcg ccaggcccgc gctcgcgtcg gacaaatccg gctggttgcg
     7081 caaagcctgc cggtactcct gctccgcgat ctcgaggtct cccgccgctt cggcaatctg
     7141 ccccgccagc agcgtgtccg acggcgtctc cggcgtcagc ttcagcgcct ccaccagcgc
     7201 atcgcgcgcc ccggtcggat cgctcttgtg cagcagcctc gccagcgccc ggtccacctg
     7261 agccttcgct gtggggttcg gtggattcgg ctccagttgc gccgccgcct gcagttgctc
     7321 aatcgcctcc gcattgcccc tggcgtttcc cttgcccgcc agcagcaacc ccagcgccga
     7381 gcgcgcctca aattgcttcg gatccgccgc caccgccttc cgataccacc cctccgcggc
     7441 atccgtgtgt ccctgcgcat cctcaacata gccccggtcg aacagcgccc gcgcatccgt
     7501 cgggtgcgcc gacaggtaga tttccagccg agctgtcgcc agtgcgtatt tcttcgcctc
     7561 gatcgccgac tccacctcgt ccagcgggct cgaggcctgc gtcggctggt cgtctcccgt
     7621 cgtccccgga ggcagaggca ccggctgcgc ccaggcagcg cgccccaggc cggccagcag
     7681 aaccaacccc agaagcaccc ctccggcgcg tccgacaaag tccgacagtc tttcctgcgc
     7741 cctgtgccct gcgctctgcg ctttgtggct cactggaaca cttcgctttc caccgcgtga
     7801 accggctcca cacccccatg ccctgccagc gcccgagcca gccactgccc cgtgtgggac
     7861 agctcattgc gcgtgatctc ttccggagtc cccgtcgcca caatctgtcc gcccgccgcc
     7921 ccgccttccg gccccagatc gatcacccag tctgcccact taataatgtc cagattgtgc
     7981 tcgatcacca ccagcgttcc tccgctgtcg atcagcttcc gaaacgccgt cagcagcttg
     8041 cccacatcgt cgaagtgtag cccggtcgtc ggctcgtcca ggatatacag cacccggctc
     8101 cgcacgcccg ccttcttccc gtccggaccc cggctttccg accgcgtcga cgccaggtgc
     8161 gccgccagct tcaccctctg tgcctcaccg ccggaaagcg tcgtcgccga ctgccccagc
     8221 cgcacgtacc ccagcccaat ctcctccaga accgccagct tatccacgat ccgcggatgc
     8281 cccgcgaaga accgcaacgc ctcccgcacc gtcatctgca gc
//
LOCUS       DASZYN010000668         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_804795, whole genome shotgun sequence.
ACCESSION   DASZYN010000668 DASZYN010000000
VERSION     DASZYN010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804795"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="VHE33_17250"
     rRNA            <1..1212
                     /locus_tag="VHE33_17250"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1378..1454
                     /locus_tag="VHE33_17255"
     tRNA            1378..1454
                     /locus_tag="VHE33_17255"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1412..1414,aa:Ile,seq:gat)
     gene            1540..1615
                     /locus_tag="VHE33_17260"
     tRNA            1540..1615
                     /locus_tag="VHE33_17260"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1573..1575,aa:Ala,seq:tgc)
     gene            1853..>2469
                     /locus_tag="VHE33_17265"
     rRNA            1853..>2469
                     /locus_tag="VHE33_17265"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          573 a          641 c          798 g          457 t
ORIGIN      
        1 cagccacact gggactgaga cacggcccag actcctacgg gaggcagcag tggggaatat
       61 tggacaatgg gggcaaccct gatccagcca tgccgcgtga gtgatgaagg ccttcgggtt
      121 gtaaagctct tttggcgggg aagatgatga cggtacccgc agaataagcc ccggctaact
      181 tcgtgccagc agccgcggta agacgaaggg ggctagcgtt gttcggaatt actgggcgta
      241 aagcgcgtgt aggcggttgt ccaagtcggg tgtgaaagcc ttgagctcaa ctcaagaaat
      301 gcactcgata ctgggcgact tgaggaccgg agaggatagt ggaattccca gtgtagtggt
      361 gaaatacgta gagattggga agaacaccag tggcgaaggc ggctatctgg acggtttctg
      421 acgctaagac gcgaaagcgt ggggagcaaa caggattaga taccctggta gtccacgccg
      481 taaacgatgg gtgctagacg ttggcgggct tgctcgtcag tgtcgtagct aacgcgttaa
      541 gcaccccgcc tggggagtac ggccgcaagg ttgaaactca aaggaattga cgggggcccg
      601 cacaagcggt ggagcatgtg gttcaattcg acgcaacgcg aagaacctta ccagcccttg
      661 acatgggagt cgccgggacc agagacggtc ccttcggttc ggccggactc cgcacaggtg
      721 ctgcatggct gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca
      781 accctcgtct ccagttgcca tcgggttatg ccgggcactt tggagaaact gccggtgaca
      841 agccggagga aggtggggat gatgtcaagt cctcatggcc cttacgggct gggctacaca
      901 cgtgctacaa tggcggtgac agtgggatgc gaaggggcga cccggagccg atcccaaaaa
      961 gccgtcccag ttcggattgc actctgcaac tcgagtgcat gaaggtggaa tcgctagtaa
     1021 tcgcggatca gcatgccgcg gtgaatacgt tcccgggcct tgtacacacc gcccgtcacg
     1081 ccatgggagt tggttctacc cgaagatggt gcgctaaccg caagggggca gccagccacg
     1141 gtaggatcaa tgactggggc gaagtcgtaa caaggtagcc gtaggggaac ctgcggctgg
     1201 atcacctcct ttctaaggaa gatcggaagc aattccggtc tcacttacac aaatcgaagt
     1261 ctggtttctc aagccggacc gccgccgtcg acgcatccct ttccatgatg atggtatctg
     1321 gcgagacgga gcgctctccg agaggacgtg tgctccgtcg cttcgtctgc ccgaaacggg
     1381 ctagtagctc agctggttag agcgcgcgct tgataagcgt gaggtcggag gttcaaatcc
     1441 tccctggccc accacttcgc gcgatcggct cgacctttcg ggaaggccgg ttcgcgccgg
     1501 ccgacgaaga tggatccaac ggcccaacgc tcgacaaacg ggggcatagc tcagttggga
     1561 gagcgcctgc tttgcaagca ggaggtcgtc ggttcgatcc cgtctgcctc caccatcatc
     1621 ccgcgcgggt caccgcgcgt cgcatcgagc ttgcgcgccg catcgagcgt cgcgttcgtt
     1681 ctttgacatc gtttatcgga agatgaggtc tgaaagtcta attccgcgct ctgtcgccta
     1741 ggcagagtgg caggaatggg tagatcaagg ccgttgcggg cgggggtcgt cccctgcacg
     1801 gaacgagccg aatgcggcga tcgcaagatc gtcgcttcgc tgacaaacgt gagatcaagc
     1861 acaagaaggg cgtttggcgg atgccttggc actgagaggc gatgaaggac gtggcaggct
     1921 gcgataagct tcggggagtt gccagcacac tttgatccga agatttccga atggggaaac
     1981 cccatccatc aggatgatcg gcgactgaat ccataggtcg acgaggcgaa cccggggaac
     2041 tgaaacatct cagtacccgg aggaaaggac atcaacagag actccgctag tagtggcgag
     2101 cgaatgcgga ccaggccagt gatacgatcg taagaaccgg aacgccctgg aaagggcggc
     2161 catagagggt gatagccccg tacgggtaga aagcggtcgt atcctcgagt agggcgggac
     2221 acgtgcaatc ctgtctgaac atggggggat caccctccaa gcctaagtac tcctcagtga
     2281 ccgatagtga acaagtaccg tgagggaaag gtgaaaagca ccccaatgag gggagtgaaa
     2341 aagacctgaa accgaacgcc tacaaacagt cggagggcgc aagcctgacg gcgtaccttt
     2401 tgtataatgg gtcagcgact tactctgtgc agcaagctta agccgatagg tgtaggcgca
     2461 gcgaaagcg
//
LOCUS       DASZYN010000669         3850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_488239, whole genome shotgun sequence.
ACCESSION   DASZYN010000669 DASZYN010000000
VERSION     DASZYN010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488239"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(413..1471)
                     /gene="obgE"
                     /locus_tag="VHE33_17270"
     CDS             complement(413..1471)
                     /gene="obgE"
                     /locus_tag="VHE33_17270"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HVT99256.1"
                     /translation="MFIDEAKIRVKAGDGGNGCMAFRREKFVPRGGPSGGDGGHGGNV
                     IMESTQRHNTLVYFRFNPEHKAERGEHGLGSNCTGRDGKDTILKVPVGTLLYDADTGE
                     LVHDFTRPDERIVIARYGRGGRGNQHFATSTHQAPREHELGRPGEERHYRLELRVLAD
                     VGLVGYPNVGKSTLISRISAARPKIADYPFTTLEPQLGVVTVGDAPYEESFIVADIPG
                     LIEGAHTGAGLGTQFLRHIERTRILVHLVDVSDASGRPDPVEDFKVITAELASFGHGL
                     DKKPIVVVASKADVANPAKLKKLETMAKRKKLPFYAISAVTGLGVDKLKHAIGDRVRE
                     LRKQSTAVEMKEAQQAAG"
     gene            1713..>3850
                     /locus_tag="VHE33_17275"
     CDS             1713..>3850
                     /locus_tag="VHE33_17275"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT99257.1"
                     /translation="MRFPRNWKAALVGLSAAIGLGLVSGATPACAGQSDTGGMVAGRI
                     FGPDGRGVAGAAVQLEATSGERSETASDLRGGFHFAAVAPGEYTLRVHTPDLSDYEAD
                     HLEVGLGTARRVNPRLAARWMHRTILVDARFVEAQVGEDAGADAGAGENASRALAGQV
                     PDNSGQWSSEAAVLSGSGSDDGEDLSLRGLSPLMSSITVDGMNGKLAFRGRQRATEGN
                     GFATVQSAVSAYRGNADELTASVRASSGAMSAVTKSGGNHMHGQAVLFDRGSFGQAAN
                     AFSKTLTLEPAETTANGQPVLYLNGQPMTYIEAPYHAPDRRQRWEVAAGGPIQRDRMW
                     WYFAWEQYQRNDPAVARANEPELFFAAPSTQSLATLEARIATSRHPLLAACPGPAPES
                     TARAECAWTNALNQLNGMLGKVPRQTRQTIVFPKLSWRVNARNQVVAQYNSMRRTGVH
                     TALSGATEMDAIGSFGNSSTSDDTGIARWDWSATPRITSSARYQYNRDLLVHAPATGS
                     AFEAQFANNAWGLPPEVTIDKSQGLSFGTLGTANKRKYPLETRQQLADGATWVHGREA
                     LRIGYDYNYVTDELEGLNGENGAYSYASLTDFLSDMLAPSSCDGTTTGTGPYPCYSRF
                     RQTVGSTNWSFETADYAAYAANEWKLGQRLTLTLGARYEYEQVPDTNAALVNADIPET
                     ARLAHDRRGFGPRGGFALDVFGKGRTVLRGG"
BASE COUNT          681 a          971 c         1468 g          730 t
ORIGIN      
        1 tatgcgtgcg atcagggcgt cttctgcttg ctgtggggtg aacctggcag tcgggtcgag
       61 tttttgcgtg ggaaggcggg ggtcccggtt ctttcccagg atggaaccgg gttcggtacc
      121 tttgattggc gcatcgcacg atttcgatgg ctattcgtgc gatcagggcg tcttctgctc
      181 gctgtggggt gaacctggcc gtcgggtcga gtttttggcg ggggcccgag ttgggttggc
      241 cgttcccgct ggctcgggtc cttcttcgct tttgaagcga atccgggact cgtttccgat
      301 ttcaggtgca atgcggagtt cgccttcgct tcaagccaat gcgggattcg tgtctgcttc
      361 tcaggaaatg ggcagatttg ggctgttccg gaacgaatcg cgatggaggc aattagccgg
      421 cggcttgctg ggcttctttc atttcgactg ccgtcgattg tttgcgaagt tcgcggactc
      481 ggtcaccgat ggcgtgttta agcttgtcga cgccgaggcc ggtgacggcg gagatggcgt
      541 agaacgggag cttctttcgc tttgccatgg tttcgagttt tttcagtttt gcggggttcg
      601 caacgtcggc cttcgaggca acaacgacga tcggtttctt gtccaggccg tggccgaagc
      661 tggcgagttc ggcggtgatc actttgaaat cttcgacagg gtcggggcgg ccgctggcgt
      721 cggagacgtc gacgaggtga acgaggatgc gagtgcgctc gatgtggcgg aggaactggg
      781 tgccgaggcc tgcgccggta tgcgcgcctt cgatgaggcc agggatgtcg gcgacgatga
      841 agctttcttc gtagggcgcg tcaccgacgg tgacgacgcc gagctgcggc tcgagggtgg
      901 tgaaggggta gtcggcgatt ttggggcgcg cggcggagat gcgcgagatg agcgtggatt
      961 ttccgacgtt gggataaccg acgagaccga cgtcggcaag gacgcggagt tcgagacggt
     1021 aatggcgctc ttcgccgggg cggccgagtt cgtgctcgcg aggcgcctga tgcgttgagg
     1081 tggcgaagtg ctgattaccg cggccgccgc ggccgtagcg agcgatgacg atgcgctcat
     1141 cgggacgcgt gaagtcgtga accagttcac cggtgtcggc gtcgtagagc aaagtgccga
     1201 cggggacttt gaggatggtg tctttgccat cgcggccggt gcagttggag cccagaccat
     1261 gttcgccgcg ctcagctttg tgctcggggt tgaagcggaa gtagaccagc gtgttgtggc
     1321 gctgggtcga ttccatgatg acgttaccgc cgtgtccgcc gtcgccgccg gagggaccgc
     1381 cgcgaggaac gaatttttcg cggcggaagg ccatgcagcc attacccccg tcaccggctt
     1441 tgacccgaat tttggcctcg tcgataaaca ttcgctgtat tcagtctatc gaaagcgcta
     1501 gcctggattc atcgagccgg aagcaggaag gaagaagcgg gaagcaggct tcgcgcaccc
     1561 gagcgatgaa agcactctgg cgtcggaatc gtgactcggg gcatcccgag gtgaacaacc
     1621 atcgccagga tcaaggtgag cgcctggaat tgaactgctt tgcgggccgg cctgtcgctg
     1681 gtctggtttt gcgctgtctt tggtgagtga aggtgcggtt cccccgaaac tggaaagcgg
     1741 ccctggtggg tctgagcgca gcgattgggc tcgggttggt ttcgggcgcg acgccggcgt
     1801 gtgcggggca atcggataca ggcgggatgg tggcgggacg gatcttcggt cccgatggac
     1861 gcggtgtggc cggggccgcc gtgcaactgg aagcgacgtc gggggaacga agtgagacgg
     1921 cgagcgatct gcggggggga ttccattttg cggcggtcgc gccgggcgaa tacacgctgc
     1981 gcgtgcatac gccggacctt tcggattatg aagcggacca tctggaggtg gggctgggaa
     2041 cggcgcggcg ggtgaatccg cgactggccg cgcggtggat gcacaggacg attctggtcg
     2101 atgcccggtt cgttgaggcg caggttgggg aggatgccgg cgcggacgca ggagcgggtg
     2161 agaacgccag ccgagcgctg gccgggcagg ttccggacaa cagcgggcag tggtcgagcg
     2221 aggcagcggt tctgtcggga tcggggtcag acgatgggga ggatttgagc cttcgtggac
     2281 taagcccgct gatgagcagc atcaccgtgg atgggatgaa cgggaagctg gcgtttcgcg
     2341 ggcggcagcg cgcgactgaa ggcaacggat tcgcgaccgt gcagtcggcg gtgagcgcct
     2401 atcgcggaaa tgcggacgag ttgactgcgt cggtgcgtgc atcgagtggc gcgatgagcg
     2461 cggtgacaaa gagcggcggc aaccatatgc acgggcaggc agtgctcttc gatcgcggaa
     2521 gttttgggca ggcggcgaat gcgttcagca agacactgac gctggaaccg gcggagacga
     2581 cggccaacgg gcaaccggtg ctttatctga atgggcagcc catgacctat atagaggcgc
     2641 cgtatcacgc cccggaccgg cggcagaggt gggaagtggc agcgggcgga ccgatccagc
     2701 gggaccggat gtggtggtac ttcgcctggg agcaatatca gcggaacgat ccggcggtag
     2761 cgcgggccaa tgaaccggag ttgttcttcg cggcgccgtc gacgcaatct ttggcgacgc
     2821 tggaagcgag gattgcgacg agccggcatc cgctgctggc ggcatgtccg gggccggcac
     2881 cggaatcgac ggcgcgggcg gagtgtgcgt ggacgaatgc gctgaaccag ctgaacggaa
     2941 tgctgggcaa ggtgccgcga cagacgcggc agacgattgt gtttccgaag ctgagctggc
     3001 gggtgaacgc gcgaaaccag gtggtggcgc agtacaactc gatgcggcgc acgggcgtgc
     3061 atacggcgct gagcggtgcg acggagatgg atgcgatcgg cagttttggc aacagctcga
     3121 cgagcgacga tacggggatt gcgcgatggg actggtctgc gacgccgcgg ataacgagca
     3181 gcgcgcggta ccagtacaac cgggatcttc tggtgcatgc gccggcaacg gggagcgcgt
     3241 tcgaggcgca gttcgccaac aacgcgtggg gcttgccgcc agaggtgacg atcgacaaaa
     3301 gccaggggct cagctttggg acgctgggga cggcgaacaa gaggaagtat ccgctggaga
     3361 cgcggcagca actggccgat ggggcgacgt gggtgcatgg acgagaggca ttgcgaatcg
     3421 gatacgacta caactacgtg accgatgaac tggaggggct gaacggtgag aacggggcgt
     3481 attcgtatgc gagcctgacg gattttcttt cggacatgct ggcgccgagc agctgcgacg
     3541 ggacgacgac ggggacggga ccgtatccgt gctattcgcg gtttcggcag acggtgggat
     3601 cgacgaactg gagcttcgag acggctgatt acgcggcgta tgcggccaac gagtggaagc
     3661 tgggacagcg gctcacgctg acgctgggtg ctcgttacga atatgaacag gtgcctgata
     3721 caaatgcggc gctggtgaac gcggacattc cggaaaccgc gcgcctggcg cacgatcgga
     3781 ggggtttcgg gccacgcggc gggttcgcgc tggatgtttt cggcaaaggg cggacggtat
     3841 tgcggggcgg
//
LOCUS       DASZYN010000670         4741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1063644, whole genome shotgun sequence.
ACCESSION   DASZYN010000670 DASZYN010000000
VERSION     DASZYN010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063644"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VHE33_17280"
     CDS             <1..998
                     /locus_tag="VHE33_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99258.1"
                     /translation="PSESQEDLPLWVRNLSVVPDTEPFIELGATADAVAEQERTAVFD
                     PPTPRVARSSPSKRTAAATAAKPVTREEKLLAAFPGMAAPASEDLPPAPLPARSKPQR
                     RTATPPPAAAKPSAEAVRVHPAASPAPAASARTPAKSKPARARARPSPPAVTTRPEVK
                     PIAPAALIGSPIQPMPASHTRRPVVKKAAAARVVKTRSRRRTKAPFRQILANTVRQPK
                     EVVAAKTKPAPDRTRRITTRFSPAEERRIEKCAAELGITVSAYLRQCTLAAATAKPQA
                     EAPDPPAPAKARKGRARGVEPAIGYAAPPPSFLGGWLALLRNRFLGAPVRFSEEA"
     gene            complement(1090..1815)
                     /locus_tag="VHE33_17285"
     CDS             complement(1090..1815)
                     /locus_tag="VHE33_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99259.1"
                     /translation="MKTWQKFALILVPAILIAAIGIWRINVSRNKPVVVPKQAQETHL
                     SDEQMVQPRKLFIDDLKSAKDLIGKPVWVQAGYEMTYYPYSGHRVDFAHKVGVLPSVQ
                     RLDVKDIVLEKVPANVETRIPRGNQQAFLVFTMPDDTKQYATAVGTIQGNDSTWYCDN
                     VLYYDDPHQMYNLWPASLWQAIDAHQPKPGMTELETAMALGVMQQSNSSDVGNRTVYY
                     NAGPKRWAVTFENDKATQVEPQK"
     gene            complement(1883..2794)
                     /gene="hemH"
                     /locus_tag="VHE33_17290"
     CDS             complement(1883..2794)
                     /gene="hemH"
                     /locus_tag="VHE33_17290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HVT99260.1"
                     /translation="MSDAVLLLAHGTPDSVDEIPEYLMRVTRGRPLPEEAVEEVKHRF
                     AAIGGSPLTRLTLEQGRLLGEELRMPVYVGMRNWNPYIADVIAKMRADGVTRAVAICL
                     APQNSRTSVGLYKKVVFDAAGDALQLRFVDHWAEHPHLIAGFAERLTATREAFGREAG
                     APVPVLFTAHSVPVRTVQPEPGTDTPDPYADQAQLTAKLVADRAGLNPGEWSFAFQSQ
                     GMSGGPWLGPTVEDTLTELRKQGTTNLVIQPIGFLCDHVEILYDIDVAFRDFAAALGI
                     TIRRPESLNDSRHLTLALADLARKELA"
     gene            complement(2787..3911)
                     /gene="hemE"
                     /locus_tag="VHE33_17295"
     CDS             complement(2787..3911)
                     /gene="hemE"
                     /locus_tag="VHE33_17295"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HVT99261.1"
                     /translation="MVLLRSFLAPGLSCNLPFVPLMTQGSSALSAHRFVRACLRQPVD
                     RTPVWFLRQAGRYMHEYMEVRRHHSLVEICKRPDLAAEVTITAAEKLDVDAAIIFADL
                     LLPLESMGLDFEFQAGEGPVIHKPLRNAADIDALVTTRAGELAYVGDAIQRVVTHFHG
                     RLGMIGFCGAPFTLASYMIEGGGSRNYIETKKLMYRQPGAWRKLLDKLVVVLKQFAAL
                     QVAAGAEVMQIFDSWAGALAIPDYREFVLPATKTLVREVQALGVPVIYFGVDTASLLP
                     AMREAGADVLGLDWRTPLDKAWRELDYSCAVQGNLDPITLFAEPDLIRTRVRQILDQA
                     AARPGHIFNLGHGIVPGTPVENVQAVVKFVREYVPEARYV"
     gene            3847..4284
                     /locus_tag="VHE33_17300"
     CDS             3847..4284
                     /locus_tag="VHE33_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99262.1"
                     /translation="MRGTKGRLQERPGARKLRNKTMARKLGEIPGRYGCEFAGNYFFF
                     DDFLALLFFDADFFFGTFAPALRASDNPIAIACLRLVTFLPLRPLLSLPVFFSRITRA
                     TFFCAFGPYFLPDDFFFAAMQHSPFLYWEAARDVQVAPESGRG"
BASE COUNT          886 a         1367 c         1577 g          911 t
ORIGIN      
        1 atccttcaga gtcgcaagag gacttgccgt tgtgggtcag gaacctgtct gtggtgccgg
       61 ataccgagcc gttcatcgaa ctgggcgcca cggccgacgc tgttgccgag caggagcgta
      121 cggcggtctt cgatcctccc actccccgcg tcgcccggtc gagcccgtcc aaacgaacgg
      181 cggctgcgac ggcggcgaag cccgtaaccc gggaggagaa attgctggcc gctttcccag
      241 gaatggcggc gcccgcgtcc gaggatctgc caccggcgcc actcccagcg cgaagcaaac
      301 cgcagcgcag gacggcgacg cctccgccag ctgcggcgaa accctctgca gaggctgtcc
      361 gggtgcatcc agcggcttca cccgcgccgg ccgcgagcgc ccgaacgcca gcgaagtcga
      421 agccggcacg ggcccgcgcc aggccttcgc cgccggccgt cacaaccagg cctgaggtaa
      481 aaccgatcgc gccagctgct ctgatcggtt cgccgattca gccgatgccg gcatcgcaca
      541 cacgcaggcc tgtggtgaag aaagcggctg cggcacgggt cgtgaagacg cgatcccgaa
      601 gacgaacgaa ggcccctttt cggcagattc tggcgaacac tgtgcggcag ccaaaagagg
      661 tagtggcagc gaagaccaaa ccggctccgg accgcacgcg ccgcatcacg acacggtttt
      721 cgcccgcgga ggagcgccgc atcgagaaat gcgccgcgga actggggatc acggtctcgg
      781 cttatctgcg gcaatgcacg ctggcggccg caaccgcgaa accgcaggcg gaagcacctg
      841 acccgcccgc gccagcgaaa gcacgcaaag ggcgggcacg tggagtggag ccagcgatcg
      901 ggtatgcggc tcctccgcca tcgtttctgg gcggatggct tgcgttgttg cgcaatcgct
      961 ttctcggggc accggtgcgg ttcagtgagg aggcgtgagg aaagcagggt ttagcgttta
     1021 gcgtatagaa gtcttcgctg ggacgcgacg tggccgagcg tggacagacg aaacgtcaat
     1081 ccctgaagcc tatttctgcg gctcgacctg cgtggccttg tcattctcga aggtcaccgc
     1141 ccagcgtttt ggtccggcgt tgtaatacac ggtccgattg ccgacgtcgg aggagttgga
     1201 ctgctgcatg acgccgagcg ccatcgcggt ctccagctcg gtcatgcctg gtttgggctg
     1261 atgcgcatcg atcgcctgcc agagactggc cggccagagg ttgtacatct ggtgtggatc
     1321 gtcgtagtag agtacgttgt cgcaatacca ggtggagtcg ttcccctgaa tcgttccgac
     1381 cgcggttgca tattgctttg tatcgtcggg catggtgaac acgaggaagg cctgctggtt
     1441 gccgcgcgga attcgagtct ccacattggc ggggaccttt tcgagcacga tgtctttgac
     1501 gtcgaggcgc tggacgctgg ggaggacgcc gactttgtgc gcgaagtcga cgcgatggcc
     1561 ggagtaagga tagtaggtca tctcgtatcc ggcctggacc cagacgggtt tgccgatcag
     1621 atccttcgcc gacttcagat cgtcgatgaa cagcttgcgc ggctgcacca tctgctcgtc
     1681 gctcaggtgc gtctcctgcg cctgtttcgg gacgaccacg ggcttgttgc gcgatacgtt
     1741 gatgcgccag atgccgatgg ctgcgatcag gatcgcgggc accagaatga gagcgaactt
     1801 ctgccaggtc ttcatggttt cagcttattg tgatgcgcag ccgtcatccg cggagcggga
     1861 aatttggcag gtccgggctc aatcaggcca gttctttacg tgccagatcg gcgagagcca
     1921 gcgtgagatg gcgcgagtcg ttcaacgatt ccgggcgccg gatggtaatg cccagggctg
     1981 ctgcgaaatc ccggaaggcc acatcgatgt cgtacagaat ctcgacgtga tcgcagagaa
     2041 acccgatggg ctggatcacc aggttcgtcg tgccctgttt gcgcaactcg gtcaatgtgt
     2101 cctcgacggt cgggcccagc caggggccgc cggacatgcc ctggctctgg aatgcgaagg
     2161 accactcgcc ggggttcaac cctgcgcggt cggcgaccag tttcgccgtt aattgcgcct
     2221 gatcggcgta ggggtcgggc gtgtcggttc cgggttcggg ttgcacggtg cgtacgggga
     2281 cgctgtgggc ggtgaacagc acgggcacgg gagcgcccgc ttccctgccg aaggcttcgc
     2341 gtgttgctgt gaggcgctcg gcgaaaccgg cgatgaggtg tggatgctcc gcccagtgat
     2401 cgacaaagcg cagctgcagc gcgtcgcctg ccgcatcgaa cacaactttc ttatagaggc
     2461 ccacgctggt gcgcgaattc tgcggcgcca gacaaattgc gaccgcgcgg gtcacgccgt
     2521 cggcacgcat cttcgcgatg acgtcggcga tgtacggatt ccagttgcgc atgccaacat
     2581 agaccggcat ccgcagctct tcgccgagca gtcgtccctg ctccagagtg agccgcgtga
     2641 gcggacttcc gccgattgca gcgaagcgat gcttcacttc ctcgacagct tcttccggca
     2701 gcggacggcc gcgggtcacg cgcatcaggt attccggaat ttcgtcgacc gagtcgggcg
     2761 ttccgtgcgc cagcagcagg acggcatcag acatagcgtg cctccggcac atattcgcgg
     2821 acgaatttca cgaccgcctg aacgttttcg acgggggtcc cggggacgat gccatgaccg
     2881 agattaaaga tatggcccgg ccgtgccgcc gcctgatcga gaatctgacg gacgcgcgtg
     2941 cggatcaggt cgggctcggc gaacaaggtg atgggatcga ggtttccctg cacggcacat
     3001 gaatagtcga gttcgcgcca cgctttgtcg agcggcgtgc gccagtccag gccgaggacg
     3061 tcggcgcccg cttcgcgcat agcagggagc aaactggcgg tatcgacgcc gaagtaaatc
     3121 acgggcacgc cgagcgcctg cacttcgcgc actaaggttt tggttgcggg cagcacaaat
     3181 tcgcgatagt cgggaatggc cagcgcgccg gcccagctgt cgaagatctg catcacctcg
     3241 gcgcccgccg cgacctggag cgccgcaaac tgtttcagaa cgacgacgag cttatccagc
     3301 agcttgcgcc acgcgccggg ctggcggtac atcagcttct tggtttcgat gtaattgcgc
     3361 gaaccgccgc cctcgatcat gtagctggcc agggtgaagg gcgcgccgca gaagccgatc
     3421 atgccgaggc gcccatgaaa atgggtgaca actctctgga tggcgtcgcc aacgtaggca
     3481 agctcgccgg cgcgggtagt caccagcgca tcgatgtcgg cggcgttgcg gagcggcttg
     3541 tgaatgaccg ggccttcgcc agcctgaaat tcgaaatcga gccccatcga ttcgaggggc
     3601 aacaacaggt cggcaaagat gatggccgcg tccacgtcga gtttttccgc agcggtgatg
     3661 gtgacttcgg cagcgagatc ggggcgcttg caaatctcta ccagggaatg gtgccggcgc
     3721 acctccatgt actcatgcat gtagcggccg gcctggcgca ggaaccacac gggtgtgcgg
     3781 tccacgggct ggcgcaggca ggcgcgaaca aatctgtgag ccgagagagc ggaggagcct
     3841 tgtgtcatga ggggtacgaa aggaaggtta caggaaagac ccggcgcgag aaaactgcgt
     3901 aacaaaacca tggcccggaa gctgggggag attccgggcc gatacggctg cgaatttgcc
     3961 ggtaattact ttttcttcga tgattttttg gcactgctct tctttgatgc cgattttttc
     4021 ttcggaacct ttgcaccggc cttgcgggcc tcggacaacc cgatagcgat tgcctgtttg
     4081 cggctggtga cttttttgcc gctgcgtccg ctcttgagtt tgccggtctt tttctcgcgc
     4141 atcacgcgcg cgactttttt ctgtgccttc ggaccgtatt tcctgcctga tgactttttc
     4201 tttgctgcca tgcaacattc tcccttcttg tattgggaag cagcgcggga tgttcaggtt
     4261 gcgccggagt cgggaagggg gtgaacctgg ttatgtcgat cgggtggtga gtagggggcg
     4321 gtggtgcggt ggctttcccg aattgggaac gggagggtac ttcggggttg gacggaggct
     4381 ggaatctctt gaccgcatgc gacgcgagag agtcttctgc ttgctgtggg gtgaacctag
     4441 ccatgtcgat aggaccgcga ggaccgaaga cgaatcggtg cagggatcga agacgaattg
     4501 gtgcggaaac gaagacgaat cggtacgagg tcgaacacca atcggtgcgg gaaaaacgaa
     4561 tcggtgtcgg aatcgaagac gaatcggtgt cggaatcgaa gacgaatcgg tgtcggaatc
     4621 gaagacgaat cggtgcggga tcgaagacta gcccgctact gcggcggcgg gcaggatgtc
     4681 ttcgacgtcg acccattgcg tgggatattc accggtatag caggcgacgc agaacttgtt
     4741 g
//
LOCUS       DASZYN010000671         1719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_488722, whole genome shotgun sequence.
ACCESSION   DASZYN010000671 DASZYN010000000
VERSION     DASZYN010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488722"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VHE33_17305"
     CDS             complement(<1..1007)
                     /locus_tag="VHE33_17305"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HVT99263.1"
                     /translation="MKRSLVLWAILATILAALPAAHAQSASHWYKQGQKAEAKGDSLG
                     AYEAYYQAYQKKPTEERYKISWERTRFAAAAIHVEQGERLRNQGNDTAALTEFLRALE
                     IDPSNELAEQDINATREKLNSQTPAPENAAKTSDEALGLAAPIELKPISNEPLTLHMV
                     EDSRIIYQAIGKAAGINVLFDPSYSSKRIQVDLNNVTLADALRIVATVSNTFWRPITS
                     NTIFVAENSRSKHTELDQQAVQTFYLHNVSQQNDFTEIQTVLRNLFSQGARINGVASE
                     NAIVMRATPDELLLAERLISDLDRPKPEVVLDVAVLEVSRDVLRNIGIQLPQTASINF
                     QP"
     gene            1241..>1719
                     /gene="smpB"
                     /locus_tag="VHE33_17310"
     CDS             1241..>1719
                     /gene="smpB"
                     /locus_tag="VHE33_17310"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HVT99264.1"
                     /translation="MARPSSHPVKQAPAKARPRDPVASGDRDAAQNRVASHNYFFLEK
                     YEAGVALRGTEVKSIREGQANLRDAYGLVKNGEAFLLNAHIGAYSHGSSNNHEALRTR
                     KLLLHQEEIQKLTVKTQQKGLTLIPTRLYFRNGRVKCEIAVARGKQDWDKRETERRR"
BASE COUNT          321 a          546 c          505 g          347 t
ORIGIN      
        1 ggctggaagt tgatgctcgc cgtctgcggc agctgaatgc cgatattgcg cagcacatcg
       61 cggctcactt ccagcactgc cacatccagc accacttccg gcttcggccg atccagatcg
      121 ctgatcagtc tctccgccag caacagttca tccggcgttg cccgcatcac gatcgcattc
      181 tcgctcgcga ccccgttgat gcgagcgccc tgcgagaaca gattgcgcag tacggtctga
      241 atctcggtga agtcattctg ctgcgagacg ttgtgcaggt aaaacgtctg caccgcctgc
      301 tggtccagct ccgtgtgttt gctccgcgaa ttttcggcga caaagatcgt gttggaagtg
      361 atgggccgcc agaaagtgtt tgagaccgtg gctacgatcc gcagcgcgtc ggccagcgtc
      421 acgttgttca gatccacctg aattcgcttg gaactatacg acggatcgaa cagaacgttg
      481 atgcccgcag ccttcccgat cgcctgatag atgatgcggc tgtcctccac catgtgcagg
      541 gtcagcggct cgttggagat cggcttgagc tcgatgggcg cagccagtcc cagcgcctcg
      601 tcggaggttt tcgccgcgtt ctccggagcg ggcgtctgtg agttcagctt ttcccgcgtc
      661 gcattgatgt cctgctccgc cagctcgttg ctggggtcga tttccagcgc gcgcaggaat
      721 tcggtcaggg ctgcggtgtc gttgccctgg ttccgcagtc tctcgccttg ctcgacatgg
      781 atcgcggcgg cagcaaagcg cgtccgctcc cacgaaatct tgtaccgctc ctcggtgggc
      841 ttcttctggt acgcctgata gtaggcctcg taagcgccca ggctgtcgcc tttggcctca
      901 gccttctgac cctgcttgta ccagtgcgac gcggactgag catgcgccgc tggaagggct
      961 gccagaatcg tggcgagaat tgcccataga accagacttc tcttcatgca gctccatcgt
     1021 tgggagaggg attggtagtg ccggaacggc tgatccctgc tctccccggt atgaagactt
     1081 ccccggtgag cgccgccgct tcgtgccgcg tatagtatcg cattcccggg aaggcctgcg
     1141 tttgacgagg caggatctgg cgcaaatggt cgccatgccc ggaaccccgg gttttcaccc
     1201 ctgtaaggcc gttatacgat gaagaattgc gacccccacc atggcccgcc catccagcca
     1261 tcccgtaaag caggctcccg cgaaggccag accccgcgat ccggtcgcgt ccggagaccg
     1321 cgacgccgcc cagaaccggg tggcctccca taactacttc ttcctcgaga aatacgaggc
     1381 gggcgtggct ctgcgcggaa ccgaggtcaa atcgatccgc gagggccagg ccaatcttcg
     1441 cgacgcctac ggcctggtca aaaatgggga ggcctttctg ctgaacgcgc atatcggggc
     1501 atattcccac ggcagctcca acaatcatga ggcgcttcgc acacgcaagc tgctgctcca
     1561 ccaggaggaa attcaaaagc tcaccgtgaa gacgcagcag aaagggctaa cgctcatccc
     1621 cacacggctt tatttccgca acggccgcgt gaagtgcgag attgccgtgg cgcgcggcaa
     1681 acaggactgg gacaagcgcg aaaccgaacg ccgccgcga
//
LOCUS       DASZYN010000672         1680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1131838, whole genome shotgun sequence.
ACCESSION   DASZYN010000672 DASZYN010000000
VERSION     DASZYN010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131838"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1598)
                     /locus_tag="VHE33_17315"
     CDS             complement(<1..1598)
                     /locus_tag="VHE33_17315"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HVT99265.1"
                     /translation="MVPKTLPLLQIVRSSVALTLLCAFTVAGSQEVPWRVQIAASPDA
                     QTLPEDRGAAGLAQTLRKLDTWASLMFIVAHPDDEDGGMMAFESRGMGARTAIMTLNR
                     GEGGQNAMSSESYDALGLIRTNELLLADQYNGSEQYFASVVDYGFSKTKEEALQQWGH
                     DRVLCDVVRAVRMYHPLVLASTFTGNITDGHGQHQVSGEVNQEAFSAAGDPNVCPDQI
                     AAGLRPWSPLKVYARVPFFPASNRGIFDYATNKWSPVRFYDYVTKQWSDTFPNATVSI
                     PEGTWDPILGESYTQIAREGWGQQKSQNGGGTMPLAGPLSVEYHRYGSLVKTSDEEKS
                     FFDGIDTSLAGIASLAHSGNTAPLVAALNDIHHHVAIALNNDLPAHPEKIAPELATGY
                     KVTQQLIAWVNSSGLSADDKANIDHELNVKLAQFNTALAQALGLQVTALVMPRTATGA
                     NPFPSLSPEDTATTVTPGSQIDVRIHVTAAPFWGPSQQGLQLARTWLDQPDNSKWQIT
                     RIGAPGLDTVSSNSGDAIFRVYVPR"
BASE COUNT          287 a          426 c          637 g          330 t
ORIGIN      
        1 cgcgggacgt agacgcgaaa gatggcgtcg ccggagttgc tgctgacggt gtcgagtccg
       61 ggggcaccga tgcgcgtaat ttgccacttg ctgttgtcgg gctgatcgag ccaggtgcgc
      121 gccaactgca agccttgctg agagggaccc cagaagggcg cagctgtgac gtggatgcgg
      181 acgtcaatct gcgagcctgg tgtgacggtg gtcgcggtgt cttcggggct caacgaggga
      241 aagggattgg ctccggtcgc ggtgcggggc atgacgagcg ctgtaacttg aagcccgagc
      301 gcctgggcga gagcggtgtt gaactgcgcc agtttgacgt tgagctcgtg gtcgatgttc
      361 gccttgtcgt cggcgctaag gccgctgctg ttgacccagg cgatgagctg ctgcgtgacc
      421 ttgtacccgg tggcgagttc gggggcgatc ttctccgggt gggcgggaag gtcgttgttg
      481 agcgcgatcg cgacatggtg gtgaatgtca ttcagagcgg cgacgagggg ggcggtgttg
      541 ccggagtgag cgagggatgc gatgccggcg agtgatgtgt cgatgccgtc gaagaaggat
      601 ttttcctcgt ccgaagtttt cacgagcgag ccgtagcggt ggtactcgac gctgagcgga
      661 ccggcgagcg gcatggtgcc gccgccattt tgcgattttt gctgacccca gccttcgcgg
      721 gcgatttgcg tgtaggattc gccgaggatc gggtcccagg ttccctcggg gatggaaacg
      781 gtcgcattgg ggaatgtgtc gctccattgc tttgtgacgt agtcgtagaa gcgaaccgga
      841 gaccacttat tcgtggcgta gtcaaagatt ccgcggttgc tcgccgggaa gaaggggaca
      901 cgcgcataga ccttgagcgg ggaccagggg cggaggccgg cggcgatctg atcggggcag
      961 acgtttgggt cgccggcggc gctgaaggcc tcctgattga cttcgccgga gacctggtgc
     1021 tggccgtggc cgtcggtgat gttgccggtg aaggtcgagg cgagaacgag agggtgatac
     1081 atgcgtacgg cgcgaacgac gtcgcagaga acgcgatcat gaccccattg ctggagcgct
     1141 tcttctttgg ttttagagaa gccgtagtcg acgacgctgg cgaagtactg ttccgagccg
     1201 ttgtactggt cggcaagcag cagctcgttg gtgcggatga gtccgagcgc gtcgtagctc
     1261 tcgctggaca tggcgttctg gccgccctcg ccgcgattca gggtcatgat ggcagtgcgg
     1321 gcgcccatgc cgcgcgactc aaaggccatc atgccgccat cttcgtcgtc ggggtgggcg
     1381 acgatgaaca tcaggctggc ccaggtgtcg agcttgcgca gggtctgcgc gaggccggcg
     1441 gcgccgcgat cttcggggag ggtttgcgca tcgggcgacg cggcgatctg cacgcgccag
     1501 ggaacttcct gagagccggc gacggtgaac gcgcacagca gggtgagcgc gacggagctg
     1561 cgaacgattt ggagaagcgg cagagtcttc ggaaccatcg aattgccagt gtaatcgcga
     1621 tgcgatttga tacaaaggca gagcatgcag gagggtacag ggtgcggcgt acagggtgca
//
LOCUS       DASZYN010000673         6347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_737475, whole genome shotgun sequence.
ACCESSION   DASZYN010000673 DASZYN010000000
VERSION     DASZYN010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737475"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VHE33_17320"
     CDS             complement(<1..369)
                     /locus_tag="VHE33_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99266.1"
                     /translation="MRSILHKFFLASAVAAAAALTTTNALADMKVNVPFSFTANGKVC
                     PAGYYSIGHDDTTGVLTLRGENWQRANFSWIGGPGEPAPTDARVVLRFDKEGDNYTLQ
                     SVQYRSLITARLDGKKHNQEA"
     gene            complement(685..1065)
                     /locus_tag="VHE33_17325"
     CDS             complement(685..1065)
                     /locus_tag="VHE33_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99267.1"
                     /translation="MRSILQKFFLASAVTAAAALATTNALAAVNVPFSFTANGKVCPA
                     GYYTIGHDGTASMVTLRSGERTFAWVITPGEPAPTDDRVILRFDKEGDQYTLQSVQYE
                     NMITARLDGKKHNNEAPSRMVLTQ"
     gene            complement(1142..2902)
                     /locus_tag="VHE33_17330"
     CDS             complement(1142..2902)
                     /locus_tag="VHE33_17330"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVT99268.1"
                     /translation="MKQFGCFRLDFVNECLWREEEQLALPPRPFMVLRYLVEHPGRLI
                     THDELLDALWPETYVQPQVLRTYMLELRKLLGDDAAEPRYIQTLPKRGYSFLAEVREC
                     VEADCGRSTKHARAAIGAGGKGIVGRDEELTRLRLQMARAEGGERQVAFVTGEVGIGK
                     TALVDAFCHELGSDWNVARGQCVLGLGEKEEYYPVMEALGRLCVPGKGDAPAGIVARM
                     APSWLRALGRHNDTVAVTNAERLPGDLCAALEQIADDKPLLLVFEDLHWGDASTLQLI
                     AALARRRAPARLMIVATCGAGDAEHPLRAMRQNLLLRQLATEVALAPLTQSAVRELLS
                     LELGQEKLPQGLTGFVHQHSEGNPLFAIAIVEHLIARRFLMREEKDAAVRWQPAGPIE
                     EIEAEVPDRVAQMIELEIESLSADEQRVLGAGSVMGIAFPAWAIAAALGEDQAAVEEA
                     CAGLARRVHFVGRGGQDELPDGTRSEFYVFVHGLYREVLYRRQAATRRAEWHKRIADR
                     LTTVFRGREWAVAREVAMHREAAGEWAAAAGTLRHAARHAKSTGAADEADALLEEAHR
                     LEVNPQNEAGPRLQIVSGKA"
     gene            3639..>6347
                     /gene="ppdK"
                     /locus_tag="VHE33_17335"
     CDS             3639..>6347
                     /gene="ppdK"
                     /locus_tag="VHE33_17335"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HVT99269.1"
                     /translation="MSTPSPVSSQALGQEIKTEAPAQYVFFFGNGVAEGNGKMRDVLG
                     GKGAGLAEMTNAGLPVPPGFTIQTEACREYMRGALSPQVTEQMDLALARLEKLQNQRL
                     GAGENPLLVSVRSGAKFSMPGMMDTILNLGLNDKSVEALAKKSNNPRFAYDSYRRLIQ
                     MFGDVVLDIPKNEFEHIFDETKKKYNAKQDTELSPVGLKEVIAGYKKLVLRHTGAEFP
                     QDPDRQLTMARDAVFRSWNNERAKTYRRLNHIDDWLGTAVNIQAMVFGNLNETSGTGV
                     GFTRNPANGEKEFYGEFLMNAQGEDVVAGIRTPVPISELRKLMPHVYDQLRDLTSRLE
                     KHYRDMQDFEFTIQDGRLYMLQTRNAKRTGLAAVRIAIDMVRENLISIDEAVLRVEPN
                     QIFDFLVPRLDEKGAIEVLAKGLPASPGAAVGQIVFTAAEAVKVHEKGTFKSIILVRA
                     ETSPEDIAGMNVASGILTSRGGMTSHAALVTRGMGKCCVAGAGDVTIDDKKKEMRVKG
                     KIFREGDWISLDGTTGRVINGKLNTLPVNPDNPDLVQFMGWVDERRRLRVLANADIPR
                     DAKQAQTFGAQGIGLCRTEHMFFAEDRLPHMQAMILAKTEEDRRAALAKLLPMQRADF
                     QGLLKVMGSYPVVIRLLDPPLHEFLPKRENLLVEIAKLEASKPKSPKLKELRKVLERV
                     EELHELNPMLALRGCRLGITYPEITEMQAHAIFEAAAAVKKSGQDPHLEIMIPLTTGP
                     EEFKAQAAIIHQVAHDVFIKKGLSVEYKVGTMIELPRACIVADKVAAEAQFFSFGTND
                     LTQTTFGISRDDCNKFLPAYLQQGIFKQDPFAVLDQEGVGTLIKMAANKGRSARRDLE
                     IGICGEHGGEPESVKFCHRAGLDYVSCSPYRLLTARLAAAQAAIEEQ"
BASE COUNT         1230 a         2223 c         1651 g         1243 t
ORIGIN      
        1 agcttcctgg ttgtgcttct tgccgtccag acgcgctgtg atcagactgc gatactgcac
       61 cgactgcagg gtgtagttgt caccctcttt gtcgaaccgc agaaccacgc gagcatcggt
      121 cggcgccggt tcgccaggac caccgatcca cgagaagttg gcgcgctgcc agttttcgcc
      181 gcgcagtgtc aggactccgg tggtgtcgtc gtgcccgatg gagtagtacc cggcggggca
      241 caccttgcca ttcgccgtga agctgaaggg gacgttgacc ttcatgtcgg ccagcgcatt
      301 cgtggtcgtc aaagctgctg ccgccgcaac ggccgacgcc aggaaaaact tgtggagtat
      361 cgatcgcatg ttgtattcct cctgcattgc tcaacacgct agcgatcccg cgtaacgcct
      421 gtcgtcaaga tcccatccag aattcgtcaa gcttttcccg acgccatctg cagccggcgg
      481 ccgtgcccat tcatcccgtc cactgcctcg ttcatcccgt cttcgactcc agagtccgct
      541 ttctcactcg ttctttctca caaaaaaggc ggctcgcctg cgcgatccgc catgtgccaa
      601 tgatggatca ggattcgtga gtgagaccca ccttgatctg cagcatccct ggggaacagg
      661 gactctcacg aatcctgctc gaagctactg ggtcaacacc attcggctgg gagcttcatt
      721 gttgtgcttc ttcccatcca gccgagcggt aatcatgttc tcgtattgca ccgactgcag
      781 agtgtactgg tcgccctctt tgtcgaaccg cagaatcacg cggtcatcgg tcggcgccgg
      841 ctcgccgggc gttatcaccc aggcaaaggt gcgttccccg ctccgcagag tcaccatgct
      901 cgcggtgccg tcgtggccga tcgtatagta cccggcgggg cacaccttac cgttcgccgt
      961 gaagctgaag gggacgttga ccgcggccaa cgcattcgta gtcgccagag ctgccgccgc
     1021 cgtgacggct gacgccagga aaaacttctg gagtatcgat cgcatgttgc ttacctcctg
     1081 ccttactcaa cacgctatcg acccgtcctc cccctgtcgt caggatcctg tctgtatttc
     1141 atcaagcttt cccggaaacg atttgcaggc ggggtcccgc ctcgttctgc ggattcacct
     1201 ccagccgatg agcctcctcc agcagcgcgt ctgcctcatc cgcagcgccg gtcgacttcg
     1261 catgacgagc cgcgtgacgc aacgttccgg cagccgcagc ccactcaccc gcagcttccc
     1321 gatgcatcgc cacttcgcgt gccaccgccc attcccgccc gcggaaaacc gttgtcagcc
     1381 ggtccgcgat gcgtttgtgc cattctgcgc gccgcgttgc cgcctgcctt cggtaaagca
     1441 cctcgcggta tagcccgtga acaaagacgt agaactccga tcgcgtccca tccggcagtt
     1501 cgtcctgccc gccacgcccg acaaagtgca cgcgccgcgc cagccctgcg catgcttctt
     1561 caaccgccgc ctggtcttcg ccgagggcgg ccgctatcgc ccacgcagga aacgcaattc
     1621 ccatcacgct gcccgctccc agcacccgct gctcgtcggc gctcaggctt tcgatctcga
     1681 gttcgatcat ctgcgccacg cgatccggaa cctctgcctc aatctcctcg atcggtccgg
     1741 caggttgcca tcgcacggcc gcatcctttt cctctcgcat caggaaccga cgcgcgatca
     1801 ggtgctccac aatcgcaatc gcgaaaagcg gattcccctc cgaatgctgg tgtacgaatc
     1861 cggtcagccc ctgcggcagc ttttcctgcc ccagttccag gctcagcaac tctcgcaccg
     1921 ccgactgggt cagcggagct aaagccactt cggtggccag ctgccgcagc agcaggtttt
     1981 gccgcatcgc gcgtagcgga tgctccgcgt cgcctgcacc gcaggtcgcc acaatcatca
     2041 gtcttgccgg ggcgcgccgc cgcgccagtg ccgcaatcaa ttgcagagtc gaggcatccc
     2101 cccagtgcag atcctcaaag accagcaaca atggcttgtc gtccgcaatt tgttccagtg
     2161 ccgcgcacaa atcaccaggt aagcgctcgg cattcgtcac agccactgtg tcattatggc
     2221 gccccaatgc acgcagccac gacggagcca tccgagccac tatcccggcc ggcgcatctc
     2281 cctttcctgg gacacaaagc cgccccagcg cttccatcac cgggtagtac tcttccttct
     2341 cgcccagacc cagcacgcac tgcccgcgcg ccacgttcca atccgacccc agttcatggc
     2401 agaacgcatc caccagcgct gttttgccaa ttcccacttc cccggtaacg aaggccacct
     2461 gccgttcgcc gccctccgcg cgggccatct gcaaccgcag ccgggtcaat tcttcgtcgc
     2521 gtccgacaat tcccttgccg cccgctccta tagcggcgcg cgcgtgcttt gtactcctgc
     2581 cgcagtccgc ttcgacacac tcacgaacct ctgccagaaa actgtagcca cgcttgggca
     2641 gagtctggat gtagcgcggc tcagccgcat cgtcgcccag cagctttctc agctccagca
     2701 tgtacgtgcg cagcacctgc ggctgcacat aggtctccgg ccacaaagca tccagcaatt
     2761 cgtcatgcgt gatgagccgg ccaggatgct caaccagata gcgcagcacc ataaagggtc
     2821 gcggaggcag cgcgagctgc tcctcttcgc gccacaggca ctcattgaca aagtcgagac
     2881 gaaaacaacc gaactgcttc attccgccgg acccctcccc ggccacctgt tcatgttggt
     2941 gatctgaccc ctccatttgt tacggatcgc gcctgaccat ccttccacca gcaaccatgc
     3001 ttccacacca tccagggccg aatgtcacga catcttcaat ccctggttgg cttctcgtta
     3061 cgattcgcat gctcccgctg tccgctcccg gacacctgtg tccgtgtttc gatgttccag
     3121 gacatctcct tgaacccttt tgtgaccaaa ctgcaaaaca gcactacccg acagacgcca
     3181 ctcctcatcg gtgagttcaa caatatatag aggggatacg caggcagacc catcttctgt
     3241 tcccgttccc cctggacatc tgcccgtcgc acacgcgatg aggagacctt caggtccggc
     3301 gcttgcctcc atccgttgtc tcctgtgatc gggcctgctt tggatttcac accgtctgcc
     3361 cttctttcct ttttctcttt tttacggcgg gcgaatcgtc ccatctcctt tttcttctgc
     3421 gccttgcggc tgttgtgatt tgcgtcacaa ccctcgtcac atgagcctgt ggcaacggtc
     3481 acatacatta tccgaccgcg aatttaacaa tcattccgtt tgagattgcg gatggcggcc
     3541 gtgcccagcg ctctcgcctc tccacttcgc ctgccgcaac gtatgtttcg cgattttcat
     3601 acaccacgcc cttaacggcg gcagaacgga gagcaatcat gagcacccct tcacccgtga
     3661 gttcccaggc actcggccag gaaatcaaaa cagaagcccc tgcccaatac gtcttcttct
     3721 tcggcaatgg cgtcgccgaa ggcaacggca agatgcgcga tgtcctcggc ggtaaaggcg
     3781 caggactcgc cgaaatgacc aatgccggcc tgcccgtccc cccgggcttc accattcaga
     3841 ccgaagcatg ccgcgagtac atgcgcggcg ctctctcgcc ccaggtcacc gaacagatgg
     3901 acctcgctct cgcgcgcctc gaaaagctcc agaaccagcg cctcggagca ggcgaaaatc
     3961 cgctgctggt cagcgtccgt tccggcgcaa agttttccat gcccggcatg atggacacca
     4021 tcctcaacct cggcctcaat gacaagtcgg tcgaagccct tgcgaagaaa tccaacaacc
     4081 cgcgcttcgc ctacgactcc tatcgccgcc tgatccagat gttcggcgat gtcgtgctcg
     4141 acattccgaa gaacgagttc gagcacatct tcgacgagac caagaagaag tacaacgcaa
     4201 agcaggatac cgagctgtcg cccgtaggcc ttaaagaggt catcgccggc tacaagaagc
     4261 tcgtccttcg ccacaccggc gccgaattcc cgcaggatcc cgatcgccag ctcacgatgg
     4321 cgcgtgatgc tgttttccgc tcctggaaca acgaacgcgc caagacttat cgccggctca
     4381 atcacatcga cgactggctt ggcaccgcgg tcaacattca ggccatggtt tttggcaatc
     4441 tgaacgagac cagcggcacc ggcgtcggct tcacccgtaa tcccgccaac ggtgagaaag
     4501 aattttacgg cgagttcctc atgaacgcgc agggcgagga cgttgtcgcc ggcatccgca
     4561 cgcccgttcc gatcagcgaa ttgcgtaagc tcatgccgca tgtctacgac cagctccgcg
     4621 acctgacctc gcgccttgaa aagcactacc gcgacatgca ggatttcgag ttcaccatcc
     4681 aggatggccg cctctacatg ctgcagaccc gcaatgcgaa gcgcaccggc ctcgctgcgg
     4741 ttcgcatcgc catcgacatg gtgcgcgaaa acctcatctc catcgacgaa gccgttctcc
     4801 gtgtcgagcc caaccagatc ttcgacttcc tcgttccacg tctcgatgag aagggcgcga
     4861 tcgaagtcct ggcaaaaggc ctgcccgcct cacccggtgc cgcagtcggc cagatcgtct
     4921 tcacagccgc cgaagccgtg aaggtccacg aaaaaggcac gtttaaatcc atcatcctgg
     4981 ttcgcgccga gacctctccc gaggacatcg ccggtatgaa cgtagcctcc ggcattctca
     5041 cttcgcgtgg cggcatgacc tcgcacgcgg ctctggtcac ccgcggcatg ggcaagtgct
     5101 gcgtggccgg cgccggcgac gtcaccattg acgacaagaa aaaggaaatg cgcgtcaagg
     5161 gtaagatctt ccgcgaaggc gactggatct cgctcgacgg caccaccggc cgcgtgatta
     5221 acggcaagct gaacaccctt cccgtcaatc ccgataaccc ggatctggtt cagttcatgg
     5281 gctgggtcga cgagcggcgc cgcctgcgcg tgctggccaa cgctgacatc ccacgcgacg
     5341 ccaaacaggc gcagaccttc ggcgcccagg gcatcggcct ctgccgcacc gagcatatgt
     5401 tcttcgcaga agatcgcctt ccgcacatgc aggccatgat tcttgcgaaa accgaagaag
     5461 accgtcgcgc cgctcttgcg aaactgctcc cgatgcagcg cgccgacttc cagggtctgc
     5521 tcaaggtcat gggctcctac ccggtcgtga tccgcctgct cgatccgccg ctgcatgagt
     5581 tcctgcccaa gcgcgaaaac ctgctggtcg agatcgcaaa gctcgaagcc agcaagccga
     5641 agtcgccgaa gctgaaggaa ctgcgcaagg ttctcgagcg cgtcgaagag ctgcatgagc
     5701 tcaatcccat gctcgctctg cgcggttgcc gactcggcat cacctacccc gagatcaccg
     5761 agatgcaggc ccacgccatc ttcgaagccg ccgcagcggt caagaagtcc ggccaggatc
     5821 cgcacctcga gatcatgatc ccgctcacca ccggccccga ggagttcaag gctcaggccg
     5881 cgatcatcca ccaggtcgcc catgatgtct tcatcaaaaa aggcctctcg gtcgagtaca
     5941 aagtcgggac catgatcgag ttgccgcgcg cctgcatcgt cgccgacaag gtcgccgccg
     6001 aggcgcagtt cttcagcttt ggcaccaacg acctcacgca gacgacgttc gggatctccc
     6061 gcgacgactg caacaagttc ctgccggcct acctccagca gggcatcttt aaacaggacc
     6121 cgttcgccgt cctcgatcag gagggcgttg gcacactcat caagatggcg gccaacaaag
     6181 gccgctccgc gcgccgcgat cttgaaatcg gcatctgcgg cgaacatggc ggcgagcccg
     6241 aatcggtgaa gttctgccac cgcgccggac tcgactacgt ctcctgctcg ccttaccggc
     6301 tgctcaccgc gcgtctcgcc gccgcgcagg cagccatcga agagcag
//
LOCUS       DASZYN010000674         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1064101, whole genome shotgun sequence.
ACCESSION   DASZYN010000674 DASZYN010000000
VERSION     DASZYN010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064101"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VHE33_17340"
     CDS             <1..407
                     /locus_tag="VHE33_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99270.1"
                     /translation="AKQCLQSNPRMIPDRGPFKERMLELQRGLVVKILVEVAQSDWHW
                     TENEYGLARELFEHCWGRRLNRAELKEALKEVCSRASEISWASLFRPFAEFPVLRKRI
                     GELETIVLRIGNLVAKIDGVWQITLARWLRKL"
     gene            529..726
                     /locus_tag="VHE33_17345"
     CDS             529..726
                     /locus_tag="VHE33_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99271.1"
                     /translation="MDDVADAVDQAPAGRIIRDSEEPARIALDRFRAKVYEAVLQAKV
                     DTAEAAFPPSDQRGHGKTVAP"
     gene            716..1879
                     /locus_tag="VHE33_17350"
     CDS             716..1879
                     /locus_tag="VHE33_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99272.1"
                     /translation="MRHKGRQPYSLLTVNGRVHLWRIRWHDSQEGSQTPTDALLDAAE
                     RALSEGVREMACRLNRGATSFQHTADNLARAAHLEVSKETLRQLIEQEGRAVLQAHRR
                     GELASDWQASDCRTESGTTRVYLGADGVMVPLITDGEKKKRREKVRQKRQRSGRRCRP
                     LPPRKEGADQQYKEFKVVTFYDENQEHRYVNATSGDHSVAGRMMQRMALQIDLKKAEE
                     KIGNVDGSPWIRNEIEFYGLVDAVGLDYYHLRDNVQKTRLVVYGEDNPVGQAWKKEMM
                     GLFYEQGYEAVWESLIGWRRSLRGAKRREADRLLGYIAQRRDMIRYPEFRQRGWQIGS
                     GPTEAQCKTSTQRLKGRGRRWDRANAEALMALDCLESSNAWHLYWTTLDPERN"
     gene            1985..>2251
                     /locus_tag="VHE33_17355"
     CDS             1985..>2251
                     /locus_tag="VHE33_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99273.1"
                     /translation="MPVEPDEMPDVLEELELDPAFGDDGEPRLKPPDEPLDPAARKRE
                     LDAALGELDRLIGLDCIKQEVKELTNFLKVQSERVRRGLPQTKVS"
BASE COUNT          497 a          633 c          728 g          393 t
ORIGIN      
        1 cggcgaagca gtgcctccag agtaaccccc ggatgatccc ggaccgcggg ccgttcaagg
       61 aacgaatgct tgagctccag cggggcctgg tcgtcaaaat cctggtcgaa gtcgcccaga
      121 gcgattggca ctggaccgaa aacgagtatg ggctggcgcg agaactgttc gagcactgct
      181 ggggcaggcg gctcaaccgc gcggagttga aagaggccct gaaagaagtc tgcagccgtg
      241 ccagcgagat cagttgggcc agtctctttc gtccttttgc cgagtttccc gtgctccgca
      301 agcgaatcgg cgaactggag acgattgtgc tccgcatcgg caacctggtg gccaagatcg
      361 acggtgtctg gcaaataact ctggcgcgct ggctgcgcaa gctgtagaac atggggaagg
      421 agtgttctgt gttctttcgg cagaatggag cgagccatgg attcgagttc ttcgagggtg
      481 gatcgtgcgg ccttgcgaga gcggttgttg gcggagttct tacggacggt ggacgacgtg
      541 gccgacgcgg tggatcaagc gccggcaggt cgcataatcc gggatagtga agagcccgcc
      601 cggatcgcct tggatcgctt tcgggcaaag gtgtacgagg ccgtcctgca ggccaaggtg
      661 gatacggcgg aagccgcttt tcccccctcc gaccagcgcg gccacgggaa aaccgttgcg
      721 ccataagggg cggcagccgt acagcctgct cacggtcaac ggacgcgtcc atttatggcg
      781 catccggtgg cacgattcgc aggaaggtag ccaaaccccg accgatgcat tgctggatgc
      841 ggcggaacga gcccttagcg agggcgttcg cgagatggcc tgccgactta accgtggcgc
      901 caccagtttc cagcacacag cagacaatct cgcgcgtgcc gcgcacttgg aggttagtaa
      961 ggaaacgctg cgtcaactca ttgaacaaga gggccgggct gtcctgcagg ctcatcgtcg
     1021 tggtgaattg gcctccgact ggcaagcctc ggattgccgc acggaatcgg gcaccacgcg
     1081 tgtctacttg ggagccgatg gcgtcatggt gccgctgatc acagacggag agaaaaagaa
     1141 gcgtcgtgaa aaggtgcgtc agaagcggca aaggagcggc cgccgatgcc gccccttacc
     1201 gcctcgcaag gaaggcgctg accagcaata caaagagttc aaagtggtaa cgttctacga
     1261 cgaaaaccaa gaacatcgct acgtgaatgc caccagcggc gaccactcgg tcgccggtcg
     1321 catgatgcaa cgcatggccc ttcagatcga cctgaaaaaa gcggaggaaa agatcggcaa
     1381 cgtggatggc tctccctgga ttcgcaatga gattgagttt tacggccttg ttgatgcggt
     1441 ggggctggac tactaccacc ttcgcgacaa cgtccagaag acacggctgg tggtttatgg
     1501 agaagacaat cctgtcgggc aggcttggaa gaaggagatg atgggactct tctacgagca
     1561 aggatacgag gcggtctggg aatcgttgat cggctggcgt cgttcactgc gcggcgcaaa
     1621 gaggcgcgaa gcggaccggt tgctgggcta tattgcccaa cgccgcgaca tgatccgcta
     1681 ccctgaattc cggcagcgcg gctggcagat cggcagtggc cccaccgagg cccaatgcaa
     1741 aacctccacc cagcgtctca aaggccgcgg ccgaagatgg gatcgcgcca acgccgaagc
     1801 actcatggcg ttagattgcc tagaaagcag taacgcttgg cacctttatt ggacaaccct
     1861 tgatcccgaa agaaattagc attgccagaa tccctggcca gacacgatcg acggcagcat
     1921 ttgcccggag gaagcggatc ggctcaagtg gattcagtcc gagttgcaac ggtgcctgat
     1981 cgccatgcct gtcgaacccg acgaaatgcc cgacgtgctt gaagagctcg agctcgatcc
     2041 ggcgttcggc gacgacggcg agccgaggct caagccgccc gacgaaccgc tcgatccggc
     2101 ggcccgcaag cgagagctcg acgccgccct gggcgaactg gatcgactta tcgggttgga
     2161 ctgcatcaag caggaggtca aggagctgac caacttcctg aaggtgcaga gcgagcgtgt
     2221 tcgacgtggc ctgccgcaga cgaaggtcag c
//
LOCUS       DASZYN010000675        25184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_737522, whole genome shotgun sequence.
ACCESSION   DASZYN010000675 DASZYN010000000
VERSION     DASZYN010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 25184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25184
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737522"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VHE33_17360"
     CDS             <1..984
                     /locus_tag="VHE33_17360"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HVT99274.1"
                     /translation="GRAPSLSIEITKECPLRCPGCYAFDAAHLGGATELRELSDFRGD
                     ELVSRILALVDERRPLHVSLVGGDPLVRYRELEQILPQLERRGIHTQVVTSAFREIPA
                     SWQQYRKLNVVVSIDGLQPEHDARRKPATYDRILKNIAGARVSVHCTITAQIAGREGY
                     LEDFMEFWSARHEVVRVWFSLFTPQRGADHEEILTPAQRAATIAELGRLRKKYAVLDL
                     PEEVLKEMQSPPKRPEECVFARTTETVSADLQTRISPCQFGGDPDCAQCGCVASMGLA
                     AVGHHRVLGPLTAGHLFRVSDRLGKTLRAFRDQQVRRPAQTEPPPFNILPS"
     gene            1028..1693
                     /locus_tag="VHE33_17365"
     CDS             1028..1693
                     /locus_tag="VHE33_17365"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HVT99275.1"
                     /translation="MTNAKSRNELELRRELVRFGRSLYRLGYMPATSGNLSVRLDENR
                     LLVTPTGASKFLLRGEDMVIVDLDGRQMEGGRRATSELGMHLAFYRERDDVQAVIHAH
                     PPVATAFACAGWALDELICQEAAMTLGPVPLARYATTGTNEVADSLRPLIPAYDTILL
                     ANHGAVACGATILSAFQKMETLEHVAQIRLVTQQLGGYNTLEQEQERQLCLARERYLQ
                     NTI"
     gene            complement(1792..2964)
                     /locus_tag="VHE33_17370"
     CDS             complement(1792..2964)
                     /locus_tag="VHE33_17370"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HVT99276.1"
                     /translation="MTRRQFGRTMLLISDRFASVSAASRFANELLSEESKLLCPVPHR
                     RAVSHRALLTPKLARGAGGAIMKLTYRLLLLVMLVVSAASITGCKRHSSAEHFYLVTA
                     NTKLAYWQSASAALDKIAREYDVHADMRGPQGFDPQDEAAEFRSVVALKPNGILVSVA
                     DEKVMQPEIDAAINAGIPVITVDSDAPSSKRLYFIGTNNRQAGRLGGHALVQKLGGKG
                     NVVFFTNPGQPNLDDRLNGYKDVLADSPNIHIKEVVDIKGVSTTAFDYTQHALPLTGK
                     DRVDAFVCLEASAGPEVGEVLRRNNAKDRVVIAMDLNTDTLEAVKQGFIAATIAQKPF
                     TMAYFGVKMLDELYHNPLPSLTKNYSVDPSAPIPAFIDTGSTLVDQSNVDQFMHPQ"
     gene            3283..3906
                     /locus_tag="VHE33_17375"
     CDS             3283..3906
                     /locus_tag="VHE33_17375"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HVT99277.1"
                     /translation="MTAGVPAPEGAGANGTVLFSFRKMRRRVGWLGGVVALLMLTAQV
                     GHGQKSFRDDVQAAYLYNFGKFVRWPQSADRGPMLVCIAGRDPIGQAVAKLVAGEEID
                     GRRVEERDLDSPEDEGACSILFVGLGEHAREDSFLMRAEGKPILTVGDGPDFLARGGM
                     IQFILVGDHVRFAVNLEAINRHRIAVSSELLKVAVKVTGNPGPGGVK"
     gene            3903..5366
                     /locus_tag="VHE33_17380"
     CDS             3903..5366
                     /locus_tag="VHE33_17380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT99278.1"
                     /translation="MRSATRSISRKLMWMNIMVSAIVLVLAVLAFFLYDVISFRQNLI
                     RDLRTAAQIVGANSITALMFDDQQSAATTLEALRNSPDVVAATLVTNDGATFAEYQRA
                     TNVPAPTAMPLAAGKIDQEWVNGSGVLLAHRIIFQGKPVGTVYIAAQLTETGKRARQY
                     LAIAFAVLLICMAAGVLISTTARRLVARPMVALAETARRVSRERDYSVRAEPQMDGDE
                     VAVLVEAFNEMLVQIQERDTALNHARGELELRVEERTAALRAANRELEAFSYMVAHDL
                     RGPLDAVQGMIYLLETSTREKQQGDGEIFEQMRQVTHGMTSLIDDLLHLSRASTAPLK
                     KELVDLTSVVARIGEDLKRGDPTRRVEFMIAHVPPVQADEGLMRVALDNLIRNSWKYS
                     SHHETACIEFGAINRRGETLYYVRDDGAGFDPKQSDELFQPFHRLHNRNEFPGTGIGL
                     ATVQRILARHGGRIWAEGAVEKGATFWFTLRGTGAAG"
     gene            5709..5951
                     /locus_tag="VHE33_17385"
     CDS             5709..5951
                     /locus_tag="VHE33_17385"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor C-terminal
                     domain-containing protein"
                     /protein_id="HVT99279.1"
                     /translation="MPDGQDRDIRLERLPMQVDVLVKVRSFRVQDLLSMQKGTVVETL
                     QEHTQDVPVRCGGALLLWSEFEVLDQRLAVRVTRLA"
     gene            6039..6314
                     /locus_tag="VHE33_17390"
     CDS             6039..6314
                     /locus_tag="VHE33_17390"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HVT99280.1"
                     /translation="MVLNPVFDGSTPMPRYSWTMRLGLWLRKLMSLPTAGELPIRLEG
                     RLSLGPKKSLVLVNCRGKSVLLALGSEVITPIMEVPAHRRRKREPAQ"
     gene            6320..7012
                     /gene="fliP"
                     /locus_tag="VHE33_17395"
     CDS             6320..7012
                     /gene="fliP"
                     /locus_tag="VHE33_17395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01103.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HVT99281.1"
                     /translation="MFLAAAKATPALPGLDLSGSLGNNNSIPWTIVFALTLLTLLPAI
                     LLSITPMVRLLIVFHFLRQALGTQTAPSNQVLLGLGLMMTWFLMQPVLVQVDQQAVEP
                     YRAGQISGWDAIVRGSGPPKAFMLRYAREKDLALFASASMTSRPAKPEDLPIRVVVPA
                     YILSELKAGFEIGAVLFLPFLLVDMVVASITTSIGMYQLPPVVISTPLKILLFVMVDG
                     WNLLAGSLLRSF"
     gene            7091..7366
                     /locus_tag="VHE33_17400"
     CDS             7091..7366
                     /locus_tag="VHE33_17400"
                     /inference="COORDINATES: protein motif:HMM:NF013480.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HVT99282.1"
                     /translation="MGPDQAGELMRHLLREALILSAPVLLAACVTSLLISLLQTLTSL
                     QDQTISTVPRLLLVVVLLLAALPWYLGRLMSYTTGLFSDFHRFIGQG"
     gene            7370..8158
                     /locus_tag="VHE33_17405"
     CDS             7370..8158
                     /locus_tag="VHE33_17405"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HVT99283.1"
                     /translation="MPALGPEGNWKQALEAMTLVLVRIGGLMVFAPVFSSTAIPPRVK
                     AGFVLAVSFLIAPIVAGFPGAHAELGIVPVLGELSVGLIFGLTLSLLFEALEFTGQVL
                     GFQFSFSLVNAMDPNSPVSTPLLGQLFGLLGTLVVLGAGLHRTVLEALFRTFATAPVG
                     TVTLDPRAGSALVMMAGGIFAAALQLSAPVMAATLLAEMAVALAGKLAPQLPVMVMDV
                     PLKTLLGYGVLIGSLALWPHWIEAHFATLLDAAGRLLARSAGKT"
     gene            8161..9285
                     /locus_tag="VHE33_17410"
     CDS             8161..9285
                     /locus_tag="VHE33_17410"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HVT99284.1"
                     /translation="MAGDRTEQATPRQRQKAAEKGDRVHSRDLMMAASMLAAVYTLGG
                     VAPKWVARWGGVYQGLLNLGQPALWERTSPPEIALAIRDIFMKALGPVGLLFVVALAA
                     ALGTGIAQGGGWAINFEALALKPERINPVENAKNVFSLRSAVRLGKSLVPVVALGFFA
                     VHAIEAQTAIPPMSVAQYPDLFAEMYNLLVEGSWIFLAWAGVDYLMEWRTREGRLRMS
                     KQDVRDEYREMEGSPQVKARIRGLRRQMRRRQLKADISRASVVVTNPTHYAVALSFDF
                     ATMEPPRVLAKGRDLLAAQIREEAQWAGVPIVENPPLARSLYRQVEPGQAIPFELYAT
                     VAAILAWLYRREVEERVRRERAEAAARGQGNSNGPGRTTR"
     gene            9282..11369
                     /gene="flhA"
                     /locus_tag="VHE33_17415"
     CDS             9282..11369
                     /gene="flhA"
                     /locus_tag="VHE33_17415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HVT99285.1"
                     /translation="MSAAIPLAITVPEGPVKTLRTLVLPVAAISVLFVMIVPVPAFLM
                     DMLLACSMAASILVFLSAVQLRRAVDFSVFPTLLLLLTLFRLSLNIASSRRILLHGNE
                     GTTAAGQVIEAFGQFVVGGNYVVGFVLFLALIAIQFLVVSHGAVRTAEVTARFTLDAL
                     PGKQMAIDADMNAGLIDEATARKRRQAIAQEAEFYGAMDGAARFNQRDALATILITAI
                     NIVAGLLIGTLQQGIDLSEAVRTYTILTVGDGLVTMIPSLLVSVAGGMVLTRASSAGQ
                     LTAELGMQLLGKGTTLYVASGVLGTLCLIPGLPKLAFLVPAAILGYMGKRAAKQEAPS
                     VEVEGAKKAVEKQENLAPSLRLDDLSLEIGFQLIPLVDEKQGGQMLGRVKTLRRHLAQ
                     ELGFLIPPVHISDNLRLKAREYVVSLRGMEIGRWQTEGNCLLAVSTEARPRPLPGKET
                     REPAYGAPARWIQPSVEEEALAAGYSVVDQGTLIGTHLAELIRKHAHELLGRAETKRL
                     LDSLSESHPKLVEELVPKVLSLGEVQKVLQQLLRERVSIRDLGTILEALVEAAAQNRG
                     LPALVEAARQALGRRLTQPMVDVDGTLRVFALEPALEEELLALVSPDASARQLTSGPP
                     TPVLRRITDSLKSLMGATSGTAQPVLLCASPARYHLRRWLEPVLPRLMVIAPAEIPPE
                     VPVRALGIVRQGG"
     gene            11379..12200
                     /locus_tag="VHE33_17420"
     CDS             11379..12200
                     /locus_tag="VHE33_17420"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HVT99286.1"
                     /translation="MDYAGQTAETWRAPGQTGKNRKRSEGAALMPEVPPLSAEERERM
                     MLEHMPAVRWMARRIHERLPQHVDLEDLVSAGVVGLLDAFRKFDPAKKVQFRSYAQFR
                     IRGAILDSLRTLDWSPRDLRRKGRAVEEAIRQITARCGRTPTEPEVAREMGLELEEYQ
                     QLLGELKGLEIGTLHLERSEDSGEEELAYIPNPSDEDPLFRCLRGEMRERLAAAIEQL
                     PERERLVVTLYYYEEMTMKEIGLALSVVESRVSQIHSSAVMHLRARLQDFAKPRK"
     gene            12213..13175
                     /locus_tag="VHE33_17425"
     CDS             12213..13175
                     /locus_tag="VHE33_17425"
                     /inference="COORDINATES: protein motif:HMM:NF014235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99287.1"
                     /translation="MEKELGQEEIDALFRAARARTEAAPEQTPRSRVLPWSFSRSGQI
                     SNEQLRAISMINDIFARNLTHHLGAWFRSRVQVSLVSAEQIPFSEFLLRIPELAYVCS
                     VRLDPLRATSVLQMDLTTAPPIIDLLLGGEGQPCEPRELTDIEEAILSGVVEAICREL
                     TTAWQPVGLSFSFEKRQMQTQTPRLIPVSEKTLCLSFELRLGETSGLLNLAFPAVASN
                     TILRRLGGEKSRQRRHSEETRRRMRQRVGACRTGTALWLPSVRVPAEALGELRPGTIL
                     RFELPASRTAAQFRAGGVTLFSAAPVGQADHRAAQLLTAPEVKG"
     gene            13172..13927
                     /locus_tag="VHE33_17430"
     CDS             13172..13927
                     /locus_tag="VHE33_17430"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HVT99288.1"
                     /translation="MNDLGFWLEKLQAEIARELGQTVSLDASGEAPQADRCLADALSL
                     PGARLILAADRDDIGAFLVEAKVIEAGQADAEMVRELWSGILASVAVGCGGTASPVGA
                     PEDQAEPWTLRLGGATVRLMAALEKSKDVTGDAPPPRGTGETAVRASRSPADASPVVK
                     RSAGNFDLLLEVELDAAVRFGSRELELKELLELGPGDVVELDRQVTDPVDLIVGDRIV
                     ARGEVVLVNGNFGLRVTEVAEPVRRLESIRCLW"
     gene            14110..14865
                     /locus_tag="VHE33_17435"
     CDS             14110..14865
                     /locus_tag="VHE33_17435"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99289.1"
                     /translation="MAAAVRREMDGSDVELPVPMHRVPMGMMLVRQGTITVDQLRAAL
                     EEQQQARRDGSEARKLGTWLLESGVLSETALTRALSLQWNCPVLSLEGFRPGEMAPAM
                     PRFLAEAFGALPVRTAGERLLYLAFSGRVDRCLTYALGRITGLQVAAGIAPESEFRPA
                     LEKYCAAAAPRVQFVETANSWALVRMIAERVESTRAAGARLVRVHDIYWLRLWRRAPD
                     KGGLPAPGRVEDLLATVGTIARNRDRTSDYRKI"
     gene            14901..15284
                     /locus_tag="VHE33_17440"
     CDS             14901..15284
                     /locus_tag="VHE33_17440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT99290.1"
                     /translation="MSGTTVLIVDDSSVMRKIVERALRQAGLNLTKVVEAGSGREGLD
                     ALAREQVDLIVSDINMPNMDGLEFLRQIQSQGLAAGVPVVMITTESGEEHVREALSAG
                     AQGYIRKPFTPDQVRERVLPLLVTA"
     gene            15326..15823
                     /locus_tag="VHE33_17445"
     CDS             15326..15823
                     /locus_tag="VHE33_17445"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HVT99291.1"
                     /translation="MSQPTPLIAGKEVEVARLEEYLDRATDEVFNTMMGVRCAPTPIG
                     PGANRESISAVIGLAGALSGSLVLQTSSEAARRMAEKMMGMAPEEVDEMVRDAVGEVC
                     NMVAGAWKGFESSLASGCLLSTPTVVAGSSYELFNQKAPIRIERGYIFETLPFTVTIS
                     CEPQG"
     gene            15896..16633
                     /locus_tag="VHE33_17450"
     CDS             15896..16633
                     /locus_tag="VHE33_17450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook basal-body protein"
                     /protein_id="HVT99292.1"
                     /translation="MDSGYYAAFSGWLARSQALDLAAGNLANAGTAGYRAERDYFRSA
                     ILGPDSLGSQLNTTVNAFGILGGSRLDFGQGALTPTGNPLDLAIEGDGFFAIQTQQGI
                     RYTRDGQLQRAQDGTLTTQAGEPLLDGKKQPIRVPAGSIAVGSDGAVSVNGAIAGKLA
                     LFTFSTPQDLAAEGANRYVAVSGAQPSAGSGAIHQGALEASNQDVIQGSLQLVLMQRQ
                     AEMMEKAVSIFSNDLDKAASQDLPRVP"
     gene            16656..17444
                     /gene="flgG"
                     /locus_tag="VHE33_17455"
     CDS             16656..17444
                     /gene="flgG"
                     /locus_tag="VHE33_17455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HVT99293.1"
                     /translation="MIRALYTAASGMSAQQLNLDTIANNLANSSTVGFRQRQLQFQDM
                     IYQNLVTPGAAQSQETVSAGLQIGLGTKSAATEIIMNQGDMNQTNNPLDLAIQGTGFF
                     QVTRPDGTMAYTRAGNFFLNNQGTLVDAQGDPVVPNITVPANATAVTITQYGVVNATL
                     PGQQNPAQLGQIQLATFVNPGGLESVGSNLFTATLSSGDPVLGNPGGTEGLGTLQQGY
                     LENSNVDVVGAFVQMVLAQRAYESNSKVIRAADDMYSEVNNMAR"
     gene            17870..18598
                     /locus_tag="VHE33_17460"
     CDS             17870..18598
                     /locus_tag="VHE33_17460"
                     /inference="COORDINATES: protein motif:HMM:NF014195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HVT99294.1"
                     /translation="MEDYRQRAMRNAVIALSLLLGCVSLPAARRREPLMGNTTPDRAL
                     KDYIARVRAEKAAEVLTPGAIWSANARLNNLVSDVKAVRVHDPIDVVVSESLAASTDG
                     TVKNQRASSASSQVAALLGKLKAGNALNDLLSQNSASSLNAQGQSVTNSSITTSLGGE
                     VADVLPNGMLVIEVVRQLTFNQQTQLIRLRGLVRPEDVNAQNQVMSTTITDMELEVVG
                     KGIVNDATYRQNPVVRFLERLMVF"
     gene            18612..19766
                     /locus_tag="VHE33_17465"
     CDS             18612..19766
                     /locus_tag="VHE33_17465"
                     /inference="COORDINATES: protein motif:HMM:NF014206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HVT99295.1"
                     /translation="MLRSRKSRIRTGTQRACCLLLIAITQLLAYADEGAKTRQARVKD
                     IASVAGVRDNQLVGYGIVVGLHGTGDSSQTVFPLQMLESALQRMGVSLQGNARASMMQ
                     TRNMAAVFLSGTLPPFSQPGTKVDVTISSAGDARSLEGGVLLLAPLYGPDGQIYAQAQ
                     GPAVLGGYAVTANGTSKSVNYPTTARIPSGAIVERGVALDLSQMRELSLFLNDADFHT
                     AEAVSADINRELGRAVAHAVDSRRIELRPEGREDIPSLLARVEEVEVEVFPRAKVVVN
                     ERTGTVVIGGNVTLQPVTILHGGLVVNVVSHFEISQPGPLAQGNTQVVQQTTVQAQDK
                     PANRIELREGATVDDLVRNLQQIGATARDIVSILQAMKEAGALEAELEVL"
     gene            19763..20143
                     /locus_tag="VHE33_17470"
     CDS             19763..20143
                     /locus_tag="VHE33_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99296.1"
                     /translation="MMILASHIAGAGAAVSVGVDSRLKPAAHEFEACLIKEFLKPLQQ
                     DALYAKKGDNGGDDDSGNALLSFGSEALARSISERGGFGIAAKIIEHLGPGKDKAGAG
                     AFSETRRFNENLPQGTRRSADEGK"
     gene            20155..20445
                     /gene="flgM"
                     /locus_tag="VHE33_17475"
     CDS             20155..20445
                     /gene="flgM"
                     /locus_tag="VHE33_17475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HVT99297.1"
                     /translation="MNVQNDLQGLQQLFSSQEVPQTSGKTGTVEPAGTKAGGADQATL
                     SSAGSLASGAPLDSDVRMEKVAAIQQAVVSGTYNVPATEVAGKMIDHMLGKQ"
     gene            20522..20770
                     /locus_tag="VHE33_17480"
     CDS             20522..20770
                     /locus_tag="VHE33_17480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99298.1"
                     /translation="METTAQASAVELLNAGIHALTHADAARLEELGDAASGCPRTEIA
                     DQQRLAQERLQILGALITLTRRNLRLFLSVSRCRVLAD"
     gene            20835..22259
                     /gene="flgK"
                     /locus_tag="VHE33_17485"
     CDS             20835..22259
                     /gene="flgK"
                     /locus_tag="VHE33_17485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HVT99299.1"
                     /translation="MATVSTAFNIAKGALDADQAALNIIANNTANVNTPGYTQQRPVW
                     VESDSTSLSGASYGTGVQMTGPQSQRDRVLLQALRQQTQDESASAARLNALNQIQALF
                     AGATSADSAASSGIGRDLSGFFSSLSSLEGNPADVASRQNVLSAATSLAQDLNGAAAQ
                     LETQRRALDGETGSAIEQANTLLKNLAQLNLQIQSTSPNQDAGTLEDQRQQDLTQLAQ
                     LIGIRTVTTENNGLTVTTMDGSLLVSQGQVHSIISGQSGGVTHYFDSQGADITSDLST
                     GGGQLGGLLAARDQDIPQVEGALDVLAYSLATQMNQLNEQGSDLNGNRGGAIFSLPAG
                     VSAANPAGSAAQIVVVMTDPIQIAAAALGGGSLDNANAIVMAKQQNAGIINGISPTAW
                     FSDIVTSLGSLTSEVSTDNTAQQAGLTQLNDQIGAISGVNLNDEAAALEQLEQAYQAA
                     SKLFTILDQVMTAALNLGVTTSYS"
     gene            22305..23201
                     /gene="flgL"
                     /locus_tag="VHE33_17490"
     CDS             22305..23201
                     /gene="flgL"
                     /locus_tag="VHE33_17490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HVT99300.1"
                     /translation="MRVNPLYINDLVGSLDATSAAQARLTQELASGSRVNALSDDPIA
                     VGQNVVLTAQIGRDDAFSQTASSIQSMLQMTDSALSTVVSQLTSAVSLATQGNNGTLN
                     ASNLQAIGNQLTGIRDEVVSLANTTYLGHYIFSGSLGSTAPFALNTSSNPAVASYQGD
                     AGVQYLETPNGQQIQLNLPGSQVFSAAGADVLGTLNALIADFSSGIVSPASQADAQSL
                     STALTHVTQQRVLIDNSLSRLQAAESYNQTEKTQLIASQTTLLQADVGKIATQLSTAE
                     TQQTALIQVIATLGKQSLFDQL"
     gene            complement(23554..24873)
                     /locus_tag="VHE33_17495"
     CDS             complement(23554..24873)
                     /locus_tag="VHE33_17495"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HVT99301.1"
                     /translation="MPVRLSPIAALLAAYQSGESNPLAEIQRSISLANGNASHNTYIA
                     LDPGWVLKEAQRQMGRAGSGRNLPLYGLPVSLKDCFDLAGFPTSIGSKFYAARNRAIA
                     SDSWVAARLRDAGAVILGKTHLHQLAYGVTGENRDYGDCLQPADATLLTGGSSSGAAA
                     SIQEDSAVAAIGTDTGGSVRAPAALCGLAGYRSSLGIGDWMGGYHLAPSFDTIGWLCR
                     DLRDLPLLGESLFELPAERPSSATLRIGVLTGPLLDGCDAAVQRSLQCWLDRLQQAAF
                     RVEPFEAPFWSEAWDIYAPIQAHEAAQIHAGFYQEFEPVIAARLEWGASLAADELQRT
                     HARLLEFRRQHNRLFEEFDFLLAPVSPLSRLSAGADHAETRARLLRLTTPASLGGNPA
                     IVLPSPDCGLQLIAPRNDDRRLLRIAAQLGDSIASAPMSFVPLSPQP"
     gene            24983..>25184
                     /locus_tag="VHE33_17500"
     CDS             24983..>25184
                     /locus_tag="VHE33_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99302.1"
                     /translation="MRNALLLMGAALLAASVPGTTTQRVQLLGQRSSPQDLQVSGELP
                     GVPAGQTRFIRYVDLSRLAQVSH"
BASE COUNT         4907 a         7051 c         8512 g         4714 t
ORIGIN      
        1 ggccgcgcac cctctctgtc gatcgagatc accaaggaat gtccgctgcg atgtcccggt
       61 tgctacgcgt tcgacgcggc acacctgggg ggcgcgacgg aactgaggga gctttccgat
      121 ttccgtggcg atgaactcgt cagcaggatt cttgcgctgg tggatgagcg ccgccctctg
      181 catgtttccc tggtaggagg cgatccgttg gttcggtaca gagaactgga gcagatcctg
      241 ccgcaactgg agcgaagggg gattcacacc caggtcgtga ccagcgcttt ccgcgagatt
      301 cctgcaagct ggcagcagta ccgaaaactg aacgtcgtgg tatcgatcga tgggctacag
      361 ccggagcacg atgcccggcg aaagccggcg acctacgacc gcattttgaa gaatatcgcc
      421 ggggctcggg tatcagtcca ttgcacgatt acggcgcaga tagccggtcg ggagggatat
      481 ctggaggact tcatggagtt ctggagtgcg cggcacgagg tcgtgcgggt gtggttcagt
      541 ctgttcacac cccagcgcgg agccgatcac gaggagatcc tgacgccggc acagcgcgcg
      601 gcgacgatcg cggaactggg acggttacga aaaaaatacg ccgttttgga cctgccggaa
      661 gaggtgttaa aggaaatgca gtccccgccg aagaggccgg aagagtgtgt ttttgcccgt
      721 acaaccgaaa cggtttcggc agatctgcag acgcggatct cgccgtgcca gtttggcggc
      781 gatccggact gcgctcagtg tggatgtgtg gcatcaatgg ggctggccgc ggtagggcac
      841 catcgagttc tggggccatt gaccgccggg catttgttca gggtttcgga tcgtctgggc
      901 aagaccctca gggcgtttcg ggaccaacaa gtgagacggc cagcacagac agaaccgcca
      961 cctttcaata tccttccatc ctgatcaacg aaggcggcta cagatgtcca attaccggat
     1021 ccagagtatg acgaacgcaa aatcccggaa cgaacttgaa ttgaggcggg aactggttcg
     1081 attcggccgc tcgctgtacc gcctggggta catgcccgcg acttccggga atttgtccgt
     1141 gcgcctggat gagaatcggc tgctggtgac ccccaccggc gcgagcaagt ttttgctgcg
     1201 cggggaggac atggttatcg tcgatctgga cgggcggcag atggagggtg gtcgccgggc
     1261 aacaagcgaa ctcggcatgc atctggcgtt ctatcgcgaa cgggacgatg tacaggcggt
     1321 catccacgca catccacctg tggcaaccgc ctttgcttgt gccggatggg cgctggatga
     1381 actgatttgc caggaagcag cgatgacgct gggccccgtg ccgcttgcgc ggtatgcgac
     1441 gacggggacg aacgaggtgg cggatagtct gagaccgctg attccggcct acgacacaat
     1501 tctgctggcc aatcacggag ccgtggcctg cggggccacg attctgagcg cgttccagaa
     1561 gatggagacg ctggagcatg tcgcgcaaat ccggctggtg actcagcaac tcggcgggta
     1621 caacacgctc gaacaggagc aggagcggca attgtgtctc gcgcgcgaac ggtatctgca
     1681 aaacacgatc tgaaatagga cgaaataagc gagaggcgac tttcaggcca acggtggccg
     1741 agtaacgcaa cagaaagtcc cgaggaacgt agagttcccg cagccctcac cctactgcgg
     1801 gtgcatgaac tgatccacgt tgctctggtc gacgagcgta gatccggtat cgatgaaggc
     1861 gggaataggc gcggacggat ccacagagta attctttgtg agcgatggca gcgggttgtg
     1921 gtaaagctcg tcgagcatct tcacgccgaa ataggccatc gtgaaaggct tctgcgcgat
     1981 cgtggcggcg ataaagccct gctttaccgc ttcgagcgtg tcggtattaa gatccatggc
     2041 gatcacaaca cgatccttcg cgttgttgcg gcgcagcact tcacccactt cggggcccgc
     2101 ggaagcttca aggcagacaa atgcgtcgac gcggtccttg ccggtgaggg gcagggcatg
     2161 ctgcgtgtag tcaaaggccg tcgtgctcac gcctttgata tcgacaactt ctttgatgtg
     2221 gatgttcgga gaatctgcca ggacatcttt gtagccattg agcctgtcgt ccaggttagg
     2281 ctgtccggga ttggtgaaga agacgacatt acctttaccg ccgagcttct ggacaagggc
     2341 atgaccaccg agtcggccgg cctgacggtt gttggtgcca atgaagtaga ggcgcttgct
     2401 gctcggcgcg tcggagtcga ccgtgatcac gggaatgccg gcgttgatgg ctgcgtcgat
     2461 ctccggctgc atgacctttt catcggccac cgaaacaaga atgccgttgg gcttcagtgc
     2521 gacgacggag cggaactcag cagcttcatc ctgaggatcg aatccctgag ggccgcgcat
     2581 gtcagcatgc acgtcgtact ctcgggcaat tttgtcgagg gcggcagagg cggattgcca
     2641 gtaagccagc tttgtgttgg ctgtaacgag gtagaaatgt tcggcactgc tgtgacgttt
     2701 acatccggta attgacgcgg cagaaacgac gagcatgacg agcagaagaa gtcgataggt
     2761 cagcttcatg attgcacctc cagcgcctcg tgcgagtttt ggcgtgagca gtgcccggtg
     2821 ggaaactgct cgccggtgtg gcacaggaca gagcagttta ctctcttcgg aaagaagttc
     2881 gttcgcaaag cgggatgccg cggaaacgga agcgaaacga tccgatatca ggagcatcgt
     2941 tcttccgaac tggcgacgag tcacgtaggc tggtaggcgg ctggtcgaaa aaggccgttt
     3001 tgaggtcgaa ccgggcgccg gactttcttc tgggacggtg tggtggcgcg cctcaagctg
     3061 gactgaggca gacggggttg gctgcacaaa cactggcaga tgacaggttc gaggcaacgc
     3121 gggatcggtc ggcacgcatc acagcactgc gcggtgcctc gtccgactcc tctggggcga
     3181 aaccgcctgg atacatttcg cgggcctgac ctgaatctgg cacccgcaac gggatcgacg
     3241 cacgccttgg ccggctttcc gaagctaaac cgagtctttg gtttgacggc aggagtgccg
     3301 gcaccggaag gcgcgggcgc aaacggaacc gttcttttct cctttcgcaa aatgcgccga
     3361 agggtcggat ggctgggggg cgttgtcgcc ttactgatgc tcacggcgca ggtgggacat
     3421 ggacagaagt cgttccgcga tgacgtgcag gctgcttatt tgtacaactt cgggaaattc
     3481 gtacgctggc cgcagtcggc ggatcgtggg cccatgctcg tttgtattgc gggacgcgat
     3541 ccgatcgggc aggcggtggc aaagttggtt gcgggagaag agattgacgg gcgccgcgtc
     3601 gaggagcggg atctggatag cccggaggac gaaggagcgt gttcgattct ctttgtggga
     3661 ctgggcgagc atgcgcggga agacagcttc cttatgcgag cggaaggcaa gccgattctg
     3721 accgtaggag atgggcccga ctttctcgca cggggcggca tgatccagtt catcctggtg
     3781 ggggatcatg ttcgtttcgc cgtcaatctt gaggcgatca atcggcatcg gattgccgtt
     3841 agttcggaac ttctcaaggt agcggtgaaa gtgacgggaa atccgggacc aggaggggtg
     3901 aaatgagatc tgccactcgt tccatctccc ggaagctgat gtggatgaac atcatggtca
     3961 gcgcgatcgt gctggtgctg gccgtgctgg cgttcttcct ttatgacgtg atttctttcc
     4021 gtcagaacct catccgtgac ctcaggacag ccgcgcagat tgtgggcgct aacagcatta
     4081 ccgccttaat gttcgatgat cagcagagcg ctgcaaccac gctggaggcg ttgcgtaatt
     4141 ccccggatgt agtggccgca acgctggtaa caaatgatgg agccaccttc gccgaatacc
     4201 agcgtgcgac gaatgtgccg gcgccgacag cgatgcccct ggcggcgggg aagatcgatc
     4261 aggaatgggt gaatggtagt ggagtcttac tcgcccaccg gatcattttt cagggaaaac
     4321 cggttggcac ggtgtacatt gcggcgcagt tgaccgaaac cgggaagagg gcgcggcagt
     4381 atctggccat tgcgtttgcg gtgcttctca tctgcatggc cgccggagtt ctcatcagca
     4441 caacggcgcg acggctggtc gcgcggccca tggtggctct ggcggagaca gcgcgccgtg
     4501 tctcgcgcga gcgcgactac tccgtccggg ccgagccgca gatggacgga gacgaagtgg
     4561 cagtgctggt ggaagcattc aacgagatgc tggtgcagat ccaggagagg gacacggccc
     4621 tcaaccacgc ccggggagaa ctggaactgc gcgtcgagga acggaccgcc gcactccgtg
     4681 cggcaaaccg tgaactcgaa gcgttttcgt atatggtggc gcacgacctg cgtgggccgc
     4741 tcgatgcggt ccagggcatg atctaccttc tggagacctc cactcgtgag aagcagcagg
     4801 gagacgggga gatctttgaa cagatgcggc aggtcactca tgggatgacg tcgctgattg
     4861 acgatcttct ccatttgtca cgggcgagca ccgcccccct gaagaaggag ctggtggatc
     4921 tcacgagtgt tgtggcccgg ataggagagg acctgaagcg gggcgacccg acacggcgtg
     4981 tcgaattcat gattgcgcat gtcccgcctg tacaagctga tgaggggctg atgcgtgtgg
     5041 ctctggacaa tctgattcgc aactcgtgga agtacagttc acaccatgag accgcgtgca
     5101 tcgaattcgg agccatcaat cgtcgcggag agacgctgta ctacgtaagg gacgatggcg
     5161 caggattcga cccgaagcag agtgatgagt tgttccagcc cttccaccgc cttcacaatc
     5221 gaaatgagtt tccaggaaca ggcatcggtc tggcgacggt tcagcggatt ctggcgcgtc
     5281 atggtgggcg gatctgggcc gagggcgctg tcgagaaagg tgcaacgttt tggttcacac
     5341 tccgtggcac cggcgcagcg ggttgagacg cggcgcgggt ctcccaacgc cgaagtcgcc
     5401 caaccccgca tttccacttc aaaggcagca aaacgatcag gcgtagtgag cacgaggccg
     5461 gtaagctgag aatcttctaa tctttgtgcc tcattctggg gcatctgcgc gcaccagccc
     5521 aggctgcgat gccgcttgca acagacgaca aacttcgaac caacggacct ccggcaggag
     5581 gtgtgccgac gggctcggct ccggtcaccg gagacactga gggggacagg tctgcccccg
     5641 tgggtccggc cgctgccgcc gccggggata ccgggctggc ggtgcgggaa acagtggcaa
     5701 acgacccaat gccggatggt caggataggg acattcgctt ggagcggctc ccgatgcagg
     5761 tggacgtgtt ggtcaaggtc cgatcctttc gcgttcagga tttgctctcg atgcaaaagg
     5821 ggaccgtggt ggagacattg caggagcaca cccaggatgt gcccgtgcgc tgcggcgggg
     5881 ctctgctgct ctggtcggaa tttgaagtcc tggatcaaag gcttgcggta cgcgtgactc
     5941 ggctggctta gaagacgtgc gccagggatg agaggcaggt tgtggaaatt gtgcgactag
     6001 cggaagaacc acgcgggcga gcgaagagga gcccgggcat ggtgctgaat ccggtcttcg
     6061 atggcagcac gccgatgccg cgctacagct ggacaatgcg cctgggatta tggctgcgca
     6121 agctgatgag tttgccaacg gccggggagt tgccaattcg cctggaagga cggctgagcc
     6181 tcggaccgaa aaagtccctg gtactggtaa attgtcgcgg caagagcgtg ctgttggcgc
     6241 tcggcagcga ggtgatcaca ccgattatgg aagtccctgc gcatcgtcgg cggaaacggg
     6301 agccggcaca gtgaacccca tgttcctggc cgccgcgaag gccacgcccg ccttaccggg
     6361 tctggacttg tcaggctctt tgggaaataa caattcgatt ccgtggacga tcgtctttgc
     6421 gctgaccctg ctgaccctgc tgccggcaat cctgctgtcg ataacgccga tggtgcggct
     6481 gctgatcgtg ttccattttt tacgccaggc gctggggacg cagaccgcgc cctccaatca
     6541 ggtgctgctg ggactcggac tgatgatgac ctggtttctg atgcagcccg tgctggtcca
     6601 ggtggatcag caggctgtgg agccctatcg cgccgggcag atctccggat gggatgcgat
     6661 cgtgcgcgga tccgggccac ccaaagcctt catgctgcgc tacgcgcgcg agaaggacct
     6721 ggcgctgttc gcttctgcgt cgatgacgtc gcggccggcg aagccggaag atctgccgat
     6781 tcgcgtcgtg gtgccggcgt acatcctgag cgagttgaag gccgggtttg agattggggc
     6841 ggtgctgttc ctgccgttcc tgctggtgga tatggtggtg gcctcgatta cgacgtcgat
     6901 cggcatgtat cagctgcccc ccgtggtgat ctcaacgccg ctgaaaattc tgcttttcgt
     6961 catggtcgat ggctggaatc tgctcgccgg atcgctcctc aggagcttct gacgtgggaa
     7021 ttggccgcgg gtgcggagga caagacgatg gaaggcgaac agggccgcgc atcggtgcga
     7081 gggagatgac atgggaccgg atcaggcggg ggaactgatg cggcatctgc tgcgagaagc
     7141 gttgattctg agcgcgccgg tgttgctcgc tgcctgcgtc acgagtcttc tgatcagtct
     7201 gctgcagacc ctgacttccc tgcaggatca gaccatttcg acggtgccac ggctgttgtt
     7261 ggtggtggtg ctgctgctgg cggctctgcc ctggtacctg gggcggctga tgagctatac
     7321 gacgggcctc ttttccgact ttcatcgctt tatcgggcag ggatgaccga tgccggcact
     7381 cgggccagaa gggaactgga agcaggcgct ggaggcgatg acgctggttc tggtgcgcat
     7441 cggcggtctg atggtcttcg cgcctgtttt ttcttcgact gcgatcccgc cgcgggtcaa
     7501 agccgggttc gtgctggcgg tgtctttttt gatcgccccg atcgtcgccg gttttcctgg
     7561 cgcacacgcc gagctgggaa ttgtgcccgt gctcggagaa ctcagcgtgg ggctgatctt
     7621 cggccttacg ctttcgctgc tgtttgaggc gctggagttc accgggcagg tcctggggtt
     7681 ccagttcagt ttttcgctgg taaacgcgat ggatccgaac tcacccgtgt cgaccccgct
     7741 gctgggacag ctgtttgggt tgctgggcac gctggtggtg ctgggcgccg ggctgcaccg
     7801 caccgtgctt gaagcgctgt ttcggacctt tgccactgcg ccggtgggaa cggtgacgct
     7861 cgatccgaga gcgggcagcg cgctggtgat gatggcaggc ggcattttcg cagcggcgct
     7921 gcaactctcg gccccggtga tggccgcgac gctgctggcg gagatggccg tggctctggc
     7981 cggaaagctg gcgccgcagc tgccggtgat ggtgatggat gtgccgctga aaacgctgct
     8041 cggctacggt gtgctgatcg gatccctggc cctttggccg cactggatcg aagcgcattt
     8101 tgcaacgctg cttgatgctg ccgggcgcct gctggcccga tcggcgggga aaacctagcc
     8161 atggcaggag accgcaccga acaggcgact ccacggcagc ggcagaaggc cgccgagaag
     8221 ggggatcgtg tacacagccg cgacttgatg atggcggcat ccatgctggc ggcggtctat
     8281 accctgggcg gcgttgcacc aaagtgggtc gcccgctggg gcggcgtcta tcagggactg
     8341 ctgaaccttg ggcagccggc gctctgggaa cgaacgtctc ccccggagat tgccctggcg
     8401 atccgcgaca tttttatgaa ggcgctggga ccggtgggcc ttctttttgt cgttgcgctg
     8461 gccgcggcac tgggaaccgg gatcgcgcag ggcggtggct gggcgatcaa ctttgaagcg
     8521 ctggccctga agccggagcg gatcaatccc gtcgaaaacg cgaagaatgt tttctcgctg
     8581 cgcagcgcag ttcgtcttgg caagtcactg gtgcctgtgg tcgcgctggg attttttgcc
     8641 gtgcatgcaa tcgaagcaca aactgcgatt ccgcccatga gcgtggcgca atatccggat
     8701 ctgttcgcgg aaatgtacaa cctcctggtc gagggatcct ggattttcct cgcgtgggcg
     8761 ggagtcgact acctgatgga gtggcgcacc cgggaaggcc ggctgcgcat gtcgaagcag
     8821 gacgtgcgcg acgaataccg ggagatggag ggcagtccgc aagtgaaagc tcgcattcgc
     8881 ggtctgcgcc gtcagatgcg gcgaaggcag ctcaaggcgg acatctcgcg ggcttcggtg
     8941 gtggtgacca atccgacgca ctacgccgta gcgctgagct ttgatttcgc gaccatggag
     9001 ccgccgcgtg tgctggcgaa aggccgtgat ctgctggcgg ctcagattcg tgaagaagcg
     9061 cagtgggcgg gggttccgat cgtcgagaac ccgccgctgg cgcgctcgct ctatcgccag
     9121 gtcgaaccgg gccaggcgat tccgttcgaa ctgtacgcga ccgtggctgc gattttggcc
     9181 tggctctatc ggcgtgaagt cgaagaacgc gtacggaggg aacgagctga agcggcggcg
     9241 cgaggacagg gcaacagcaa cggtccagga aggacaacgc gatgagtgca gcgattccct
     9301 tagccatcac cgtgccggaa ggccctgtca aaacgctgcg cacgctggtc ttgccggtgg
     9361 cggcgatcag cgtactgttt gtgatgattg tgccggttcc cgccttcctc atggacatgc
     9421 tgcttgcttg ttcgatggcg gcttcaatcc tggtcttcct ctccgccgtg cagctgcgtc
     9481 gagccgtgga cttttcggtc ttccctacgc tgctgctgtt gctgacgctg tttcgccttt
     9541 cgttgaatat cgcatcgagc cggcgcatcc tgctgcacgg caacgaaggt acgacggccg
     9601 ccgggcaagt gatcgaggcc ttcgggcaat tcgtcgtggg tggaaattac gtggtggggt
     9661 tcgtgctgtt tctggccctg atcgcgatcc agttcctggt cgtgagccat ggtgcggtcc
     9721 gcacggccga ggtgacggcg aggttcaccc tcgatgcgct gcccggcaaa cagatggcaa
     9781 tcgacgccga catgaatgca ggcctgatcg atgaagccac ggcacgcaaa cggcggcagg
     9841 cgatcgccca ggaggcggag ttctatggtg cgatggatgg ggcagcgcgc tttaaccagc
     9901 gagacgctct ggccacaatc ctgatcacgg cgatcaacat cgtggccgga ctgctgattg
     9961 ggacgctgca gcagggcatc gacctgagcg aagccgtccg gacatacacc atcctgaccg
    10021 tgggagatgg cctggtcacg atgatcccct ctctgctggt gtcggtcgcc ggtggcatgg
    10081 tgctgacccg tgcctcctcg gccgggcaac tgacggccga actgggaatg caactcctcg
    10141 ggaaaggaac gaccctctac gtcgccagcg gtgtcctggg cactctgtgt ctcatccccg
    10201 ggctgccgaa gctggctttt ctggtaccag ccgccatcct tggctacatg ggcaagcggg
    10261 cggcgaagca ggaagctccg tctgttgagg tcgagggcgc gaaaaaggct gtcgagaagc
    10321 aggaaaatct ggcgccttct ttgcgcctcg atgatcttag cctcgagatc gggttccagc
    10381 tgattccgct ggtggacgag aagcagggtg gtcagatgct gggtcgagtg aagaccctgc
    10441 gacgccacct ggcgcaggaa cttggttttc tgattccgcc tgtgcatatc tccgacaacc
    10501 tgcgtctgaa agcgcgcgaa tacgtggttt cactgcgcgg gatggaaatt gggcgctggc
    10561 agacggaagg gaactgcctg ctggcggtct cgaccgaggc ccgtccacgc ccgcttccgg
    10621 ggaaggagac acgggagccc gcctacggcg cgccggcccg ctggatccag ccgtctgtgg
    10681 aagaggaggc cctggcggcc ggttactcgg tcgtcgatca gggaactctg atcgggacgc
    10741 atctcgcgga actgattcga aagcatgcgc acgaactgct gggtcgcgcc gagaccaaac
    10801 gtctgctgga ctcgttgagc gagagccatc ccaagctggt cgaagaactg gtgcccaaag
    10861 ttctctcgct cggcgaagta caaaaagtgt tacagcagtt gctccgcgaa cgggtttcga
    10921 ttcgcgatct gggcaccatt ctcgaggcgc tggtggaagc cgcagcacag aatcgcggtc
    10981 ttccggcgct ggtcgaggcg gcgcgccagg cgctgggacg gaggctgacg cagccgatgg
    11041 tcgatgtgga cggtacgttg cgggtctttg ccctcgaacc ggccctggag gaagaactgc
    11101 tggcgctggt ctccccggac gcctcggcac ggcagttaac cagcgggccg cccacgcctg
    11161 tcctgcgccg catcaccgat tctttgaaat ccctcatggg cgcgacgtcc ggtacggccc
    11221 agcccgtgct cctctgtgcc agtccggcac gttaccacct gcggcgatgg ctggagccgg
    11281 ttctgccgcg gctgatggtg attgctccgg ccgagattcc accggaagtg ccggttcggg
    11341 cgctgggtat tgtgcgccag gggggatgag atcacgcgat ggactacgca ggtcagacag
    11401 cagagacgtg gagagcgccg ggacagaccg gaaagaaccg gaagaggagc gagggcgcag
    11461 cgctgatgcc ggaggtcccg cccctcagcg ctgaggaacg cgaacgaatg atgctggagc
    11521 atatgccggc ggtacgctgg atggctcgcc ggattcatga gcgcctgcct cagcatgtgg
    11581 accttgagga tctggtgagc gccggtgtcg tgggcctgct ggacgccttt cgcaaattcg
    11641 atcccgcgaa aaaggtgcag tttcgcagtt acgcgcagtt ccggattcgc ggagcgattc
    11701 tcgacagtct gcggaccctg gactggagtc cccgcgatct gcgtcgcaaa ggaagagcgg
    11761 ttgaagaggc gatccgccag atcacggccc ggtgcggccg caccccgacc gagccggagg
    11821 tggcacggga gatgggcctc gagctggagg aataccagca gctgctgggc gagctcaagg
    11881 gcctcgaaat agggacgctg catctggaac gttcggagga ttccggcgag gaggaactgg
    11941 cctatatccc caatccttcg gacgaggatc cgctcttccg ctgtctgcgc ggggagatgc
    12001 gggagcggct ggcggccgcc atcgagcagc tcccggaacg agagcgtctg gtggtgacgc
    12061 tctactacta cgaggagatg acgatgaagg agatcgggct ggcgctgagc gtggtcgagt
    12121 cgcgggtgtc gcagatccat tcctcagcag tgatgcatct tcgggcgcgg ctgcaggact
    12181 tcgcaaagcc ccggaaatag aacgggtgaa tcatggaaaa ggaactcggt caggaggaaa
    12241 tcgacgcgct gtttcgggcc gcccgggcac gcaccgaggc cgccccggag cagacccccc
    12301 gcagccgcgt tttgccatgg agcttttccc gatccgggca aatctcgaac gaacagctgc
    12361 gcgctatcag catgatcaat gatattttcg cccggaatct gacgcatcat ttgggcgcgt
    12421 ggtttcgcag ccgggtgcag gtcagcctgg tctcggccga acagattcct ttcagtgagt
    12481 tcctgttgcg gattccggag ctggcctacg tatgctcggt gcgtctggac ccgctgcggg
    12541 ccacctcggt tcttcagatg gacctgacca ccgcgccgcc gatcattgac ctgctgctgg
    12601 ggggcgaggg acaaccgtgc gaaccgcgtg agctgaccga catcgaggag gcgatcctct
    12661 ccggggtggt ggaggcgatc tgccgtgagc tgaccacagc ctggcaaccg gtggggctga
    12721 gcttcagttt cgagaagcgc cagatgcaga cgcagacgcc tcgcctgatt ccggtgtcag
    12781 agaaaactct gtgcctgagc ttcgagctgc gtcttggaga aacgagcggg cttctgaatc
    12841 tcgcgtttcc cgctgtagcc tcgaatacga ttctccgccg tctgggggga gaaaaatccc
    12901 ggcagcggcg gcactcagag gaaacacgac ggcggatgcg gcagcgggtc ggggcctgcc
    12961 ggactggaac tgcactctgg ctgccttcgg tgcgcgttcc cgccgaagcc ctgggtgaat
    13021 tgcgcccggg aacgatcctg cgattcgagc tccccgcatc ccgcacagcc gctcagtttc
    13081 gcgccggcgg cgtgaccctg ttttcggccg cgccggtcgg acaggccgac catcgtgcag
    13141 cccagttgct gacggcgccc gaggtgaagg gatgaacgat ctcggtttct ggctggagaa
    13201 gctgcaggcg gagatcgcga gggagctggg ccaaacggtc tcgctcgatg cgtcaggaga
    13261 agctccccag gcggatcgct gtctcgccga tgcactcagc ctgccgggag cccgtctgat
    13321 cctggccgcc gaccgcgacg acattggcgc atttctggtg gaagcaaagg tgatcgaagc
    13381 cggtcaggcg gacgccgaga tggtacgcga actctggtcc ggcatcctgg ccagcgtggc
    13441 tgtcggctgc ggagggactg catcgcctgt gggcgctccg gaggaccagg ctgagccctg
    13501 gacgctgcgc ctgggcggtg cgacggtgcg cctgatggcg gctctggaga aatcgaaaga
    13561 cgtcactggc gatgcgcccc cgccgcgagg aacgggggaa acagcggttc gggcctcccg
    13621 ctcgccggca gacgcttcgc cggttgtgaa gcggtcggcc ggcaatttcg atctgctgct
    13681 cgaggtcgaa ctcgatgcgg cggttcgttt cggctcccgc gaactcgaac tgaaagagct
    13741 tctggaactc ggtcccggcg acgtggtcga actggaccgc caggtgaccg atccggtgga
    13801 cctgattgtc ggggaccgca ttgtggcgcg tggagaagtc gtcctggtga acggcaattt
    13861 tgggctgcgc gtgaccgagg ttgccgagcc ggtgcggcgt ctggagagca tccgatgcct
    13921 ctggtaacag gtgccgaaac gcgggaaagc cgcgctgcgc ggcgcgcggt cggtgcgttg
    13981 gccggaacac tattcccgcg ctgtgccaat ccggaatgtg ccagcgggtg gatgcatgtg
    14041 tggagaaacc gccagacacc tgttttcgaa ggaaagtggg tctgttcggc tgcgtgcatg
    14101 gagtcgctgg tggccgctgc cgtccgccgg gagatggacg gaagtgacgt ggagctgccc
    14161 gtgccgatgc atcgggtgcc gatggggatg atgctggtcc ggcagggcac gatcacagtg
    14221 gatcaacttc gggcggctct ggaggagcag cagcaggcga ggcgggacgg cagtgaggcg
    14281 aggaagctgg gaacgtggct gctcgaaagc ggcgtgctct ccgagacggc cctgacgcgc
    14341 gccctgagcc tgcagtggaa ttgcccggtg ctctctttgg aaggatttcg ccctggagag
    14401 atggcgcccg cgatgccgcg attccttgcc gaagctttcg gggcgctgcc cgtgcgcaca
    14461 gccggggaac ggcttttgta tcttgcattc tccggacggg tcgatcgttg cctgacttat
    14521 gctcttggac gaatcaccgg acttcaggta gcggctggca tcgcaccgga atccgaattc
    14581 cgcccggcgc tcgagaagta ctgtgctgcc gccgcgccgc gggtacagtt cgtggagaca
    14641 gccaactcct gggctctggt gcggatgatc gccgagcgcg tcgagtccac acgcgcggcc
    14701 ggggctcgcc tggtgcgggt ccatgacatc tactggctgc gcctctggcg gcgcgcgccc
    14761 gacaaaggtg gtttgcccgc gccgggaagg gtcgaagatc tgctggcgac ggtaggaacc
    14821 atcgcccgaa atcgagatcg cacttcggat tacagaaaaa tctgagcccg ccgataagag
    14881 aagccgagag ggaaagcgcg atgtcgggaa caaccgtgct gattgtcgat gattcctcgg
    14941 ttatgcggaa gattgtcgag cgggcccttc gccaggcagg cctgaatctg acaaaagtcg
    15001 tggaggcggg cagcgggcgc gaagggctgg atgctctggc gcgcgagcag gtcgatctga
    15061 tcgtctccga catcaatatg ccgaatatgg atggcctcga gtttttgcgt cagatccaga
    15121 gccagggcct ggctgcgggg gtccctgtgg tgatgatcac aaccgagagc ggcgaggagc
    15181 acgtgcgcga agctctgtcc gccggggccc agggatatat ccgcaagcct tttaccccgg
    15241 atcaggtcag ggaacgcgtt ctgcctctgc tggtcacagc ctgatcgcga tgcaaaacgc
    15301 aattaacacg ccagggaagg ggacgatgag ccagccaaca ccgctgatcg caggcaaaga
    15361 ggtggaagtc gcgcgactgg aagaatatct ggatcgggcg accgatgagg ttttcaatac
    15421 catgatgggg gttcgctgtg cgccgacgcc catcgggccg ggagccaacc gggaatccat
    15481 ctcggctgtc atcgggctcg ccggggccct gagtggatcg ctggtgctgc aaaccagcag
    15541 cgaagcggcg cggcgcatgg cagaaaagat gatggggatg gctccggagg aagtcgacga
    15601 gatggtgcgc gacgcggtcg gcgaggtctg caacatggtc gccggagcat ggaagggttt
    15661 tgagtcctca ctggcgtcag gctgcctgct gtcgacccca accgttgtcg cggggagcag
    15721 ttatgagctg ttcaaccaga aggctccgat ccggatcgag cgcggatata tcttcgaaac
    15781 gttgcccttt acggtaacga tcagctgcga gcctcaggga tgaggcgttc ctaattttcg
    15841 ggtccgcctc gcggttattc tcctgcacct gctgcaccgt gggaggggaa gcgcggtgga
    15901 cagcgggtat tatgctgcat tctccgggtg gctggcgcgt agccaggccc tcgatctggc
    15961 ggccggtaac ctggccaacg ccggaaccgc cggataccgg gcggaacgag actacttccg
    16021 ttcggcaatt cttggccctg attccctcgg ttcgcaactg aacaccacgg tgaatgcctt
    16081 cgggatatta gggggcagcc gtctggactt cgggcagggg gctttgaccc cgaccggcaa
    16141 tcccctggac ctggcgattg agggggacgg gttctttgca attcagaccc agcagggcat
    16201 ccgctacaca cgcgacgggc aactgcaaag ggcgcaggac ggaaccctga cgacccaggc
    16261 cggcgagccg ctcctcgatg ggaaaaaaca accgatccgt gtaccggccg gatcgatcgc
    16321 cgttggctcc gacggagcgg tctcagtcaa cggtgcgatc gccggcaagc tggcactctt
    16381 cacgttttct accccccagg atctggcggc ggagggggcc aatcgctatg tagctgtctc
    16441 cggcgcccag ccctcggccg ggagcggagc catccatcag ggagccctgg aggcttccaa
    16501 tcaggatgtg attcagggca gcctgcaact ggtgctgatg cagcggcagg cggaaatgat
    16561 ggagaaggcc gtctcgatct tctcgaacga cctggacaag gcagccagcc aggacctgcc
    16621 gcgtgtcccg tgaggcaccg gcatgaggag gaaccatgat tcgagcgtta tacacagcag
    16681 ccagcggcat gagcgcacag cagctgaatc tggacacgat cgcgaacaat ctggcgaact
    16741 cgtcgacggt cggctttcgc cagcgacagt tgcagttcca ggacatgatc taccagaacc
    16801 tggtgacgcc gggagccgcg cagagccagg aaaccgtctc cgcaggcctg cagatcggcc
    16861 tgggaacaaa gtcggcggcg accgagatca tcatgaatca gggcgacatg aaccagacca
    16921 acaaccctct ggacctcgcc attcagggca caggattctt ccaggtgacg cggccggatg
    16981 gaacgatggc ttacacccgt gcggggaact tttttctgaa caatcaggga acgctggtgg
    17041 atgcgcaggg cgacccggtg gtgccgaaca tcaccgttcc ggccaatgcg actgccgtca
    17101 ccatcaccca gtacggcgtc gtcaatgcaa ccctcccggg ccagcagaac cccgcccagc
    17161 ttgggcagat ccagctggcg acctttgtca atcccggagg cctcgaaagc gtgggctcca
    17221 acctgtttac ggccaccctc tcctcgggtg atccggtgct ggggaatccg ggcggaaccg
    17281 aagggctggg cacactccag caggggtacc tcgaaaactc gaacgtggac gtggtgggag
    17341 ccttcgtgca aatggtcctg gcgcagcgcg cgtatgaaag caattcgaag gtgatccgag
    17401 cggcggatga catgtatagc gaagtcaaca atatggcgcg gtgaccgatg gacggacggt
    17461 ggattctcgg agcctggttc ctcctggcgc cgctggcgca gggggcctgc agcaacgcaa
    17521 cgatcgtgcg ggactggggg ttgcatcaag cctggcgagt gcagcggaat tgcgcgcggc
    17581 cggagcggcc agcggttttg gtggaggtcc cgtggacgga cccggatccg atacggcgcg
    17641 gtgctcctgg ctcaggttcg gctcagctgc cactgatccg tgcgggtatg agagtggtgc
    17701 tgtcctggca ggacaggaac agtgcaggaa gccttgtcgg cacggctttg acaacaggcg
    17761 gcgccggcga aacggtccga gtccgcagcc ggttcggcgt gacggttctg cagggggtgg
    17821 tgcgtgggcc cggctgggtc gaactggaat ccggaaaggt cggcaggtga tggaggacta
    17881 tcgacagcgt gcgatgcgca atgcggtcat cgcgctttcc ctgctgttgg gctgtgtctc
    17941 cctgccggcc gcacgtcgca gggagccact gatgggtaac acgactccgg atcgggcgct
    18001 caaggactac atcgcacgcg tgcgcgcgga gaaggccgcc gaggtgctga cccccggagc
    18061 gatctggtcc gccaacgcgc ggctgaacaa tctggtgagc gacgtgaagg cggttcgggt
    18121 ccacgatccc atcgacgtcg tggtgtccga gagcctggct gcatcgaccg atggaacggt
    18181 gaaaaaccag cgggcttcga gcgccagttc acaggttgcc gcactgctgg gcaagctgaa
    18241 agccggcaac gcgctgaatg atctgctgag tcagaattcg gcctcttcgc tgaacgccca
    18301 gggccagagt gtgacgaact ccagtatcac gaccagcctg ggaggtgagg tcgccgatgt
    18361 gctccccaat gggatgctgg tgatcgaggt ggtgcggcag ctgacgttta atcagcagac
    18421 gcagctgatc cgcctgaggg gcctggtgcg gccggaagat gtcaacgcac agaaccaggt
    18481 catgtcgacc acgatcaccg atatggaact cgaggtggtc ggcaaaggca tcgtcaatga
    18541 cgccacctac cggcagaatc cggtagtgcg ttttctcgaa cggctgatgg ttttctaggg
    18601 tggtggggac gatgcttcga agcaggaaga gcagaattcg gacaggaacc cagcgagcgt
    18661 gctgtctcct gctcattgcg ataacgcagc tgcttgcgta tgcggatgaa ggcgccaaga
    18721 cgcgacaggc acgggtcaag gacatcgcct cggtggcggg ggtacgcgat aaccagttgg
    18781 tggggtatgg gatcgtcgta ggtctgcatg gaaccgggga cagttcgcag acggtttttc
    18841 ctctgcagat gctggagtca gcccttcagc ggatgggagt gagtctgcag ggcaacgcaa
    18901 gggcttcgat gatgcagacg cgcaacatgg cggcggtttt tctttccgga actctcccgc
    18961 ctttttccca gcccggcacc aaagtggatg tgacgatttc ctcagcaggc gatgcaagga
    19021 gcctcgaagg tggcgttttg ttactggccc cgctgtacgg accggacggg cagatttacg
    19081 cgcaggctca gggccctgcg gtgttgggcg gctatgcggt gaccgcgaac gggaccagca
    19141 aatcggtgaa ttatccgacc acagcgagga tcccgtccgg tgccattgtg gagagaggcg
    19201 tcgcgctcga tctgtcgcag atgagggagc tgtcgttgtt tctgaatgac gcagattttc
    19261 acacagccga ggcagtgtcg gcggatatca atcgagagtt aggccgggcg gtggcgcatg
    19321 cggtcgacag tcggcgcatc gaactccggc cggagggcag agaggatatc ccatctcttc
    19381 tggcgcgggt ggaagaggtt gaggtcgagg tttttccgcg agcgaaagtg gtcgtgaacg
    19441 aacgcaccgg aaccgtggtc atcggaggca acgtgacgct gcaaccggtc accatcctgc
    19501 acggaggttt ggtggtcaat gtcgtcagcc atttcgagat ctcccagcca gggccgctgg
    19561 ctcaggggaa cacgcaggtg gttcagcaga cgaccgtgca ggcccaggac aaaccagcca
    19621 atcggatcga gctaagagag ggagcgaccg tagacgatct ggtcaggaat ctgcagcaga
    19681 tcggtgcaac ggcgcgcgat atcgtctcga ttctgcaggc gatgaaagaa gccggggcac
    19741 tcgaagccga gttggaggtc ttgtgatgat cctggccagc catattgcgg gagccggagc
    19801 agcagtgtcc gttggggtgg actcgagact gaaaccggcg gcacatgaat tcgaggcctg
    19861 cctgattaag gaatttctga aaccgctaca acaggatgcg ctctatgcaa aaaaaggaga
    19921 caacggcggc gacgatgaca gcggcaatgc cctcctgagt ttcggatccg aggcgctggc
    19981 gcgttcaatc tccgagcgcg gtggtttcgg gatagcagcg aagataatcg aacaccttgg
    20041 acctggaaag gacaaggccg gcgccggtgc attctcagaa acgcgccgat tcaatgaaaa
    20101 tcttcctcaa ggtacgcgaa gatcggccga tgaagggaaa tgaaggagac caacatgaac
    20161 gtacagaacg accttcaggg attgcagcag ctgttttcga gccaggaagt accgcaaacg
    20221 tccggcaaga ccgggacggt ggagccggca ggaacaaaag cggggggcgc tgaccaggcg
    20281 actttgagct cggccggtag tttggcttcg ggagcacccc tggactctga tgtgcgcatg
    20341 gagaaggtgg ctgccatcca gcaggcggtg gtcagtggca cgtacaacgt gcctgcgacg
    20401 gaagtcgcgg gaaagatgat cgaccatatg ctggggaagc aatagaacca ggcgacgtgg
    20461 cctgcgttga tgtgccgtgc aggctgaggg tgcgaagcac gggacaccca ggggggagcc
    20521 aatggaaaca acagcgcaag cctcggccgt tgaattactg aacgccggta ttcatgcgct
    20581 gacccatgcc gacgccgctc ggctcgagga attgggcgac gccgcatctg gctgtccgcg
    20641 aacagaaata gcggaccaac agcgactcgc gcaggagcgg ctgcagattt tgggggctct
    20701 gattaccctc acccggcgca acctgcggct gttcctcagc gtcagccgat gtcgcgtttt
    20761 ggccgattga agccaaaaga tccgagccgc gggccatcac ggtaagcccg ggcgactcgg
    20821 agaattgagg gcggatggcg accgttagta cagcgttcaa cattgcgaag ggcgcactcg
    20881 atgcggacca ggcagcgctc aacatcattg cgaacaacac agccaacgtg aacactccgg
    20941 gctatacgca gcagagaccg gtctgggtag aaagcgattc aacttcgctc agcggcgcca
    21001 gctatggtac aggcgtgcaa atgaccggac cgcagtcgca gcgcgatcgg gttctgttgc
    21061 aggcactgcg tcagcagacc caggatgaga gcgcctcggc agcgcgactg aatgcgctca
    21121 atcagattca ggcattgttt gcgggagcga cgagcgcaga ttcggccgcc agcagtggta
    21181 ttggaaggga cttgtccggc ttcttcagct cgctgtcctc tttggagggg aaccccgcgg
    21241 atgttgcgtc gcgccagaac gtgctgagcg cagccaccag cctggctcag gacctgaacg
    21301 gcgctgcagc ccagcttgag acgcagcggc gcgcgctcga cggggagact ggaagcgcga
    21361 tcgaacaggc caacacgctg ttgaagaatc tcgcgcaact aaacctgcaa atacagtcca
    21421 ccagcccgaa tcaggatgcg gggacgctgg aggatcagcg gcagcaggat ctcacccagc
    21481 tcgcccagct gatcggcatt cgtacagtga ccacggaaaa caacgggctg acagtgacca
    21541 cgatggatgg atcgctgctg gtttctcagg gccaggttca ctccatcatc agcggccaga
    21601 gcggcggggt tacgcattac ttcgacagcc agggagccga tatcacgtcg gatctgagca
    21661 cgggaggagg acagctggga ggcctgctgg ctgcccggga tcaggatatc ccgcaggtcg
    21721 aaggcgcatt ggatgtcctg gcctattcac tggcgacgca aatgaaccag ctgaacgagc
    21781 agggctcgga tctgaacggg aatcgtggcg gggccatttt cagcctgcca gccggggtat
    21841 ccgcggccaa cccagccggt tccgcagcac aaattgttgt cgtgatgacc gatccgattc
    21901 agatcgccgc agcggccctg ggcggcggtt ctctggataa cgccaatgca atcgtgatgg
    21961 cgaaacagca gaatgcaggc atcatcaacg ggatttcacc gacggcctgg ttttctgaca
    22021 tagtgaccag cctcggcagt ctcacctcgg aagtctcaac ggacaacacc gcgcagcagg
    22081 cgggactgac gcagttgaac gaccagattg gagcaatttc cggggtgaac ctcaacgatg
    22141 aagcggcggc tctggaacag ttggagcagg cctaccaggc agcctcaaaa ctgttcacca
    22201 tactggatca ggtaatgacc gctgcactga atcttggcgt gacaaccagt tactcatgag
    22261 tcagcgagac gaggccgtcg cgaagcggca gaagcgagac aggaatgaga gtcaatccgc
    22321 tgtacatcaa cgatttagtc ggctcgctgg atgcgacctc ggcggctcag gcaaggctga
    22381 cgcaggaact ggccagcgga tcgcgtgtga atgcgttgag tgacgacccg attgcggttg
    22441 gccagaacgt cgttctgact gcgcagatcg gtcgcgatga cgcgttttca cagactgctt
    22501 cgtccattca aagtatgctc cagatgacgg actcggcact gagcaccgta gtcagccaac
    22561 tgacgtcggc tgtgtcattg gccacacagg ggaacaatgg gacgctcaac gccagcaacc
    22621 ttcaggccat cggcaatcag ctgacgggaa tccgggacga agtggtttcg ctggccaaca
    22681 cgacctatct cggccactac attttctcgg gcagcctggg aagcacggct cccttcgctc
    22741 ttaataccag ttccaatcct gctgtagcga gttatcaggg tgatgctggc gttcagtacc
    22801 tggagactcc gaacgggcaa cagatccaac tcaatcttcc cggcagccag gttttctcgg
    22861 cggccggcgc ggatgttttg ggtacattga atgcgctgat cgccgacttt tcatccggga
    22921 tagtgtcgcc agcgtcccag gcagatgcgc agtcgctgag cacagcgctc acccatgtga
    22981 cccagcagcg agtgctgatt gataattcac tgtcgcggct gcaggcggcg gagagctata
    23041 accagactga gaagacgcag ttgatcgcct cgcagacgac gttgttacag gcggatgttg
    23101 gcaagattgc tacacaattg agcacagcag aaacccagca gaccgcgctg attcaggtga
    23161 ttgcaacgct tgggaagcaa agtctgttcg accagctgta gccgattttc cgttgcctgc
    23221 agtttgaacg gcccgtatct ggtgcctctc gacgtcctga gtcagcgcga ttcagttcga
    23281 tgccaacgcc ggtttttcca ggagcgaggc ggagcctctc aaagccccgc agtccctcac
    23341 cggacgcttc accttttccg tcggatcgtc ggagcggcca tggcgccgtc acgatggcca
    23401 ggagaaggca accgaaaagg aagctacagg tgcacgcagg caatggcgag aatcgtggcg
    23461 cgttcgagcc gcatcgggtc gcgtgcgccg cgcttccggc atagttttgt cgattgcttg
    23521 tgggcgaggg gccgctccct cctcaccctg cgcttatggc tggggcgaca ggggaacgaa
    23581 cgacatcggt gcagaggcaa ttgagtcgcc gagttgcgct gcgatgcgca acagccgccg
    23641 atcatcgttg cgaggggcaa ttagttgaag gccgcagtcc ggagaaggaa ggacgatggc
    23701 cgggttgccc ccgaggctgg ccggagtggt gagccgcaat aggcgggccc gggtttcggc
    23761 gtggtcagct ccggcagata agcggctgag aggcgagacg ggggcaagga gaaaatcaaa
    23821 ttcttcgaac aggcggttgt gctgccgccg aaactccaac agccgcgcgt gcgtccgctg
    23881 gagttcgtcc gccgcgagcg acgcacccca ttcgagacga gcggcgatca ccggctcgaa
    23941 ctcctgataa aagcccgcgt ggatctgtgc ggcttcatgg gcctgaatcg gggcatagat
    24001 atcccaggct tcagaccaga aaggagcctc gaacggctcg acacggaagg ccgcctgctg
    24061 caggcgatcc agccagcatt ggagagagcg ttgcactgcg gcatcgcacc catcgaggag
    24121 cggaccagtg aggacgccga tgcgaagcgt tgcggacgag ggccgttcgg cgggcagttc
    24181 gaagaggctt tccccgagca gcggcaaatc acgaagatca cggcagagcc agccgatggt
    24241 gtcaaaggat ggcgccagat gataaccgcc catccagtcg ccgatgccga gcgacgaccg
    24301 atagccggcg agcccgcaga gagcggcggg agcgcgaacc gaaccgccag tgtcggtgcc
    24361 gatggcggcg acagccgaat cctcctggat ggaagccgcc gcaccgctgg aggagccgcc
    24421 ggtgagaagt gtcgcgtctg cgggctgcag acagtcgccg tagtcgcgat tttctccagt
    24481 gacgccgtag gcgagttggt gaagatgggt tttgccaagg atgaccgcgc ctgcatcgcg
    24541 cagccgtgca gcaacccagg aatccgatgc gatggcgcga ttccgggcag catagaattt
    24601 cgatccgatg ctggtgggaa atccggccag gtcgaagcag tctttgagcg agaccgggag
    24661 gccatagagc ggcaggtttc tgccgctgcc ggcgcggccc atctgtcgct gggcttcttt
    24721 gaggacccag cctggatcaa gcgcgatgta ggtgttatgg ctggcgttgc cgttcgccag
    24781 cgagatcgag cgttggattt cggcgagggg attggactcg ccagattggt aggctgcgag
    24841 gagcgcggcg atgggcgaga ggcgcaccgg catcccgcca ttttaaaagg cgcatgcagc
    24901 gttgggcccg cgggaaccgg tacgcggccg acctgctcag gtttcggtgc aagggtggac
    24961 aaggtcgttt agcatagccc gcatgcgaaa cgcactgctt ctgatgggtg cggccctgct
    25021 ggccgcatca gtccccggga caacgaccca gcgcgtgcaa ctcctgggac agagaagctc
    25081 accgcaggat ctgcaggttt caggagagtt gccgggagtt ccggcggggc aaacgcgctt
    25141 tattcgctac gtcgatttga gccgcctggc ccaggtgagc catc
//
LOCUS       DASZYN010000676         1843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_167521, whole genome shotgun sequence.
ACCESSION   DASZYN010000676 DASZYN010000000
VERSION     DASZYN010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167521"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VHE33_17505"
     CDS             <1..787
                     /locus_tag="VHE33_17505"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HVT99303.1"
                     /translation="NETMLLSSASNRPPGGANTGKFPSPPAGTVNEHRIQMIYQLSAR
                     FPQIVRSVLTGMSASGALLIACSFGFTSPVQTAVTVAQVRVPMSGEDRQALAAEQQDA
                     ADHIWHLKHMAHLAHLEVLQEQEAEAQAHAAWLKQQEARHAAAVRYAAAHPARTPVKT
                     TAHSQPASPVTKPKAATPAAHYTGSSAMQACIIRAESGGNSQIWNASGHYGLYQFSEQ
                     TWAAHGGNPADFGHASVAEQNAVFAQTVADDGYSDWTPYDGCL"
     gene            1400..1831
                     /locus_tag="VHE33_17510"
     CDS             1400..1831
                     /locus_tag="VHE33_17510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99304.1"
                     /translation="MEINWTPGIIGICLLAGAVIAFAVYRRMERQRQAAGERYLWNRD
                     GYAEWQPGELQQFKTDAWAASVADEPAPVRFAGAQAATERFEQLRQTAAELHREPPTA
                     EIPVITDARLTPPPPPAKEDPFITKLRAENDRFRQLWALQP"
BASE COUNT          370 a          629 c          574 g          270 t
ORIGIN      
        1 aaacgagacc atgctgttat cgtctgcatc taacaggccg cctggcgggg cgaataccgg
       61 caagtttcct tccccgcctg ccggcacagt caacgagcac aggatacaga tgatctacca
      121 gctcagcgca cgtttcccgc aaatcgtgcg ctctgttctg acaggcatgt cagcatccgg
      181 cgccctcctc atcgcctgca gtttcggctt cacctccccc gtccagacgg cggtaaccgt
      241 cgcccaggtc cgcgtcccca tgtccggcga ggacaggcag gcgctcgcgg cagagcagca
      301 ggacgccgct gaccacatat ggcacctgaa gcacatggcc cacctcgctc accttgaggt
      361 gctgcaagag caggaagccg aggcgcaggc gcacgccgcg tggctgaagc agcaggaggc
      421 ccggcacgca gcggccgtcc ggtacgccgc cgcgcacccc gcccgcaccc cggtgaagac
      481 cacggcccac agccagccgg cctcacctgt gacgaaaccc aaggcggcaa cgccggctgc
      541 gcactacacc gggtccagcg ccatgcaggc gtgcatcatc agggccgagt ccggcggcaa
      601 cagccagatc tggaacgcct ccggtcacta cgggctctac cagttctccg agcagacctg
      661 ggccgctcac ggcggcaacc cggccgactt cgggcacgct tcagtggccg agcagaacgc
      721 ggtgttcgcc cagaccgtcg cagacgacgg ctacagcgac tggactcctt acgatggatg
      781 cttatgaaat gtgttagcat aagctatgcg agttcagccg ccctctgtgg gtgacacctt
      841 cggccgctgg acggtgaccg gcagctccta catgagagat gccggtcacc gggtaacccc
      901 gtgccgctgc tcttgcggaa ctgagcgaga ggtactcacc gccacgctcc ggagcggcaa
      961 atccgtgagc tgcggctgct ggaaacgaga agaccttgcc gcacgaaagc ccgctacaac
     1021 acacggtaag cggcatcatc ccctctatca gctctggaag cgggtcaacc gccggtgtta
     1081 cgacccgaat tcgcacaact accgctggta cggtgcgcgc gggatcggcg tctgggagcc
     1141 gtggcggaag gacgccggtg ctttcattgc ctacatcgag ggtgagctag ggccgtgccc
     1201 tgaaggagca tcacttgaca ggattgacaa caacggtaac tacgaaccgg gcaacctccg
     1261 ctgggcgact gctaccgagc aggcgagaaa ccggggaaga gattaggtat gcgtacggcc
     1321 ccatgacagt gccgtcaacg ggtactcgaa aagcagaagc aggtgctaaa gtggcatctg
     1381 taaacgaatg gagctacccg tggaaatcaa ctggacaccc ggcatcatcg gcatctgcct
     1441 gctggccggg gccgtcatcg ccttcgccgt ctaccgccgg atggagcgcc agcgccaggc
     1501 cgccggggag cgttacctgt ggaaccgcga cgggtacgcc gagtggcagc cgggcgagct
     1561 gcagcagttc aagaccgacg cctgggccgc cagcgtcgca gacgagcccg ccccggtccg
     1621 gtttgcagga gcgcaggcag ctaccgagcg gtttgagcag ctgaggcaga ccgcagctga
     1681 gctgcaccgg gagccgccga ccgccgagat cccggtcatc accgacgccc gcctcacccc
     1741 gccgccgccc ccggcgaagg aagacccgtt catcacgaag ctccgcgccg agaacgaccg
     1801 cttccggcag ctgtgggcac tgcagcctta gtgccacgtg gca
//
LOCUS       DASZYN010000677         2796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_99114, whole genome shotgun sequence.
ACCESSION   DASZYN010000677 DASZYN010000000
VERSION     DASZYN010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99114"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1460)
                     /locus_tag="VHE33_17515"
     CDS             complement(<1..1460)
                     /locus_tag="VHE33_17515"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT99305.1"
                     /translation="MVSRRRRYRELAESMQEHLAERIDILVSQGLPRHEAEFRARREF
                     GNMTHIEEQSREVWQWPRIESLWADVKYALRQMRRSKGFAAAAIGTLALGIGAATAMF
                     TVVDHVLLRPLPYKDAGQVVAMDEHGPDPRFHSSQPWVDIAEWRARSRSFDDIAFWTR
                     MNGRSYVAGNSAALQVDAEEVSSNLFAMLGARTAIGRGFIGEAPSFSAVKNTGSIVLS
                     DQVWHAAFGGDAGIVGKIVRVNDQPYTVVGIMPPAFSLPAGMDQYGQVWTTIQLGTND
                     NSREDRGAKFYNVLARLKKGVKLAAAQAEMATIQTQVAAGYKDPETRASHTRVDVAPY
                     GDSLIDKDVKKALLALLAAAGVLWLIASVNVTNLLLARATARQREIAMRGALGASRKR
                     LLQQMLVEGLLLSVFAAVLGTGLALLAVRFTRSVAPVHLNVDFSTQVDWRILAGLCGL
                     TLLTAVLASAWPALMAAWAPIEPALKQGGQQTGAGRR"
     gene            complement(1483..>2796)
                     /locus_tag="VHE33_17520"
     CDS             complement(1483..>2796)
                     /locus_tag="VHE33_17520"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVT99306.1"
                     /translation="LLFALGVSLLSGVLFGLAPALTTARGDLLSPIREGGRTDTGGRH
                     RLGSAVIVGQVALGIVLTAVAGLLVTSYAHLTHSDQGFNPDHVLTLLFETPDARYAHT
                     RPQFYRQYFEKVRALAGVEAAGGSMLLPMTDNSAHISFEDPEHPVSKATRPGATLDIV
                     SDGYFHTMQIPLVAGRDFTMADTETSPQVMIVNQAFADKFFHGENVLGKKLRPGAGNG
                     QPEGPPWREIVGVVGNIRTEALSREVEPMYFLPASQMSTWCCLYTVIRTRVDPASLEP
                     QVRSVVASMDPDIPVTDVHTMHDLIGLQLMSARFATILLSAFSILALVLTVVGLYGVM
                     AYSVARRTREIGVRLALGAQRGTVLTMVLRQAALLLAIGVAIGIAATLFATPVLHSML
                     YGTGARNPLVLAGVCVLVTMTGLVAAYLPALRAAGVNPTEALRAE"
BASE COUNT          499 a          953 c          845 g          499 t
ORIGIN      
        1 cgccggcccg cgccggtttg ctgaccgccc tgtttcagtg ccggctcaat cggagcccat
       61 gcggccatca gcgctggcca tgccgaagcc agcaccgcgg tcagcagcgt gagcccgcac
      121 aggccggcaa gaatccgcca gtccacctgc gtggaaaaat cgacattcag gtgcacaggc
      181 gcgacgctcc gagtgaagcg cacagcaaga agcgcaagcc cggtcccgag gacagctgcg
      241 aagacgctca acagcaggcc ctcaacgagc atttgctgca gcaggcgttt gcggctcgcg
      301 cccagcgccc cgcgcatggc gatctcgcgc tgccgcgccg tcgctcgcgc cagcagcaga
      361 ttcgtcacgt tcacgctggc gatcagccaa agcacacccg cagcggcgag cagggccagt
      421 agcgccttct tcacatcttt gtcgatcagc gaatcgccgt agggcgccac gtccacccgc
      481 gtatgactcg ctctcgtttc ggggtcctta tagcccgccg ccacctgcgt ctggatcgtt
      541 gccatttccg cctgcgcggc agccagcttc acgcctttct tcagccgcgc cagcacgttg
      601 taaaacttcg cgccgcggtc ttcccggctg ttgtcgtttg tgcccaactg aatcgtggtc
      661 catacctggc catattggtc catgcccgcc ggaaggctga aggctggcgg catgatgccc
      721 acgacggtat acggctgatc gttgacgcgt acgattttgc cgacgatgcc ggcatcgccg
      781 ccgaaggccg catgccagac ctggtcgctc agcacaatcg agcccgtgtt tttgactgcg
      841 ctgaaactgg gtgcttcgcc gatgaagccg cgcccgattg ccgttctcgc accgagcatc
      901 gcgaacagat tggagctgac ctcctctgcg tcaacctgta gagcggcact gtttcccgcc
      961 acgtagctgc gcccgttcat ccgtgtccag aacgcgatgt cgtcgaaaga acgactccgt
     1021 gcacgccact ctgcgatgtc gacccagggc tgcgaactat gaaagcgggg atcgggcccg
     1081 tgttcatcca tcgcgaccac ctgccccgcg tccttgtacg gcagcggccg caggagcacg
     1141 tgatccacca cggtgaacat cgccgtggcc gcgccgatgc cgagcgcgag cgtcccaatc
     1201 gccgctgctg cgaatccttt actcctgcgc atctgccgca gcgcgtattt cacgtcggcc
     1261 cagagcgact cgatccgcgg ccattgccag acttcgcgac tttgctcctc gatgtgcgtc
     1321 atgttgccaa actcccttct cgcgcgaaac tccgcttcgt gccgcggcag gccctggctt
     1381 accaggatgt cgatgcgttc ggccagatgc tcctgcatcg actctgccag ttctcgataa
     1441 cgcctgcgcc tggagaccat cgctctctcg tgtgtttgaa gcttactcgg cccgcaatgc
     1501 ctccgtcgga ttcacgcccg cggcgcgcag ggccggcaaa tacgcagcga ccaggccggt
     1561 catcgtcacc agcacgcaca cgccagcgag caccagcgga ttccttgcgc ctgtgccata
     1621 cagcatggag tgaaggaccg gcgtggcgaa gagggttgcc gcgattccga tcgcgacgcc
     1681 gatcgccagc agcagggccg cctgtcgcag caccatggtc aacaccgttc cgcgctgggc
     1741 gccgagtgcg agacgcacgc cgatctcccg cgtgcgccgc gctaccgagt acgccatcac
     1801 cccgtataga ccgaccacgg tgagcaccag ggccaggatc gagaacgcgc tcagcagaat
     1861 cgtcgcgaac ctcgccgaca tgagctgcag tccgatcaga tcgtgcatgg tatgcacgtc
     1921 agtgacgggg atgtcgggat ccatggatgc caccacgctg cgcacctggg gctcgaggct
     1981 tgccggatcc acccgcgtgc gtatcaccgt gtagagacag caccaggtgg acatctggct
     2041 ggcgggcagg aagtacatcg gttcaacctc ccgcgacagg gcctcggttc gaatgttgcc
     2101 cacgactccg acaatttccc gccacggagg accttcgggt tggccgtttc cggcgccggg
     2161 cctgagtttc tttcccagca cgttctcgcc gtggaaaaac ttatctgcga aggcctggtt
     2221 gacgatcatc acctgcggcg aggtctcggt atccgccatc gtgaagtcgc gcccggccac
     2281 caggggaatc tgcatggtgt ggaagtagcc gtcggagacg atgtcgagcg tcgctccagg
     2341 ccgcgtggcc tttgacaccg gatgctcggg atcctcgaag ctgatgtgcg cgctgttgtc
     2401 cgtcatgggc agcagcatcg atccgcctgc tgcttcaacg ccggccagcg ctcgcacttt
     2461 ttcaaagtac tgccggtaga actgcgggcg ggtgtgagca tagcgagcat cgggcgtttc
     2521 gaacaacagc gtgagcacat gatccggatt gaagccctga tcgctgtggg tcaggtgggc
     2581 atagctggtc accagcagtc ccgccaccgc cgtgagcacg atgcccagag ccacttgtcc
     2641 gacaatgacg gccgacccca gccgatgacg cccgccggtg tcggttcgtc cgccttcccg
     2701 gatcggcgac agcagatcgc ctcgcgcggt cgtcagcgcg ggcgccaggc cgaagaggac
     2761 gcccgacagc aaggagacac cgagcgcgaa caggag
//
LOCUS       DASZYN010000678         1848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1064481, whole genome shotgun sequence.
ACCESSION   DASZYN010000678 DASZYN010000000
VERSION     DASZYN010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064481"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VHE33_17525"
     CDS             complement(<1..309)
                     /locus_tag="VHE33_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99307.1"
                     /translation="MKRVWEAVVLLAGVAGLTIAGWHRRKVLVQRDQLRRVRRKIVID
                     AEGFHAQGMSVAWAALDAVWAYKRDLFAYDEICVLFHHGAGQALEVGEHDEGFMDLLK
                     E"
     gene            466..969
                     /locus_tag="VHE33_17530"
     CDS             466..969
                     /locus_tag="VHE33_17530"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HVT99308.1"
                     /translation="MRPKGSSAALEQRRRCAVAMLKQGMEPGAVAKAVHASTVSVGRW
                     RKAVREGGAKALAARPVPGRPPKLDPARRPEIVQLLLRGPIHYGFATELWTLARIAEV
                     IERHLGVDYHPSQIWRILSGLGWSCQKPESRARERDEQAVLRWRRSVWPRIKKSGRDG
                     KKRAFSG"
     gene            980..1471
                     /locus_tag="VHE33_17535"
     CDS             980..1471
                     /locus_tag="VHE33_17535"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVT99309.1"
                     /translation="MLQPLVRRTWAPRGDRPVMYCWDRHDRLSVIAGLTLSAKRHRVG
                     LCFAIHDQNIKTDEVEQFIHDVKRQVRRELIVVMDRLPAHRSAARRLIADGFEIEWLP
                     GYAPDLNPTEALWSQTKYADMANFVPEDTLALELEAQFSLETTQSDQRLLKSFFHAAR
                     LSI"
BASE COUNT          400 a          588 c          513 g          347 t
ORIGIN      
        1 ttccttcaac aggtccataa atccctcatc atgctccccc acttccaaag cctgtccggc
       61 gccatgatgg aaaagtacgc agatttcatc gtatgcaaac aggtcccgct tataagccca
      121 aaccgcatcc agcgctgccc aagccaccga catgccctgt gcatgaaatc cttcggcatc
      181 aatcacaatc tttcgacgca cccgtcgtag ttggtctcgc tgtaccagca ccttgcgtcg
      241 atgccatcca gcgatagtca gccccgctac acccgcaagc aaaacaacgg cttcccacac
      301 gcgcttcaac cccgcaccag acgcttaaat atatcaacgg tacatcgcgg ccccacaacc
      361 tccaagttcg aaattgaggc cgccatactt actgctcttt gaaaaaataa tgtaactcaa
      421 tcgcacgccg cctacgatga cttgtctcca ggacaggagt ctgcgatgag gccgaaagga
      481 tcttcggcgg cattggagca gcgtcgccgg tgcgccgtgg cgatgctgaa gcagggcatg
      541 gagcccggtg ccgttgccaa ggcggtgcat gcatcaacag tgtcggtcgg acgctggcga
      601 aaggccgttc gcgaaggcgg cgccaaggcg ctcgcagccc ggcccgtgcc tggcaggcct
      661 cccaaattgg acccggcccg gcgcccggag attgtccagt tgctcttgcg cggcccgatc
      721 cattacggct ttgcgaccga gctctggacg ctggcgcgaa tcgccgaagt gatcgagcgg
      781 catctggggg tggattatca tcccagccag atctggcgaa tcctttcggg cctgggatgg
      841 agttgccaga agcccgagtc ccgggcgcga gaacgggacg aacaagccgt tctccgctgg
      901 cgcaggagcg tctggccgcg cataaaaaaa agcggccgag acgggaaaaa gcgtgctttt
      961 tctggatgag acgggattga tgctccagcc cctggtacgc cgcacctggg ctccccgggg
     1021 agaccgcccg gtgatgtatt gctgggaccg ccacgaccgc ttgagcgtca tcgccggcct
     1081 gacgctctcg gccaaaaggc accgcgtggg cctctgcttc gccatccacg atcagaacat
     1141 caagaccgac gaggttgagc aattcatcca tgacgtgaag cggcaggtgc ggcgggagct
     1201 gatcgtggtg atggatcgcc tgccggccca ccgctcggcg gcccggcgac tgatcgccga
     1261 cggattcgaa atcgagtggc tgcccggcta cgctcccgat ttgaatccca ccgaagcgct
     1321 ctggagccag accaaatatg cggacatggc caactttgtg ccggaggaca cgctggcgtt
     1381 ggaactggag gcgcagttct ccctggaaac cacccagagc gaccagagat tgctgaaatc
     1441 cttttttcat gctgccaggc tatccatatg atctgttaca ttactttttc aaagggcagt
     1501 aatggggctc gccacggaaa atgttcctac aactttgcct tctccctagc ccccgcacgt
     1561 cgcatcccct gcacgtcggg gccttctttt tctcctgcgg gtagctcggg ctgtaccact
     1621 ttaccaaagc aggcagtcag cagcctttcc gccgagcagt tctcgaccta ccgtgcgaag
     1681 gcaaagatcg ccacgatgaa caagatcaag accagcagac agccgcagcc gaccaacccg
     1741 cagccgtcag tggcggcgtt tccttggcct cgacgatacc gatggcccat gtagaagagg
     1801 cggctaaaca ttgcagacct cccagaatca gaacgcttaa acggggtc
//
LOCUS       DASZYN010000679        11342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_865516, whole genome shotgun sequence.
ACCESSION   DASZYN010000679 DASZYN010000000
VERSION     DASZYN010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 11342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11342
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865516"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..617)
                     /locus_tag="VHE33_17540"
     CDS             complement(21..617)
                     /locus_tag="VHE33_17540"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HVT99310.1"
                     /translation="MSGSLPQPASFPAHPTRIILTGFMGAGKTTVGALLAERLGWDFF
                     DSDVLVETREAMTIADIFQRRGETAFRDAEAAVIREALACRPGEPFVLAVGGGALERS
                     DTRDFLASLHDTLIVFLEAPLETMIARCAGHECGPVRPVLADRARLVERWNKRLPWYR
                     QAHLTIDTANLAPEAVVERILGDLDRHATASSKAGATA"
     gene            complement(658..930)
                     /locus_tag="VHE33_17545"
     CDS             complement(658..930)
                     /locus_tag="VHE33_17545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99311.1"
                     /translation="MTVIRAMDKTMTCSEFQDALPELFETQADLSGQEHLKSCENCAA
                     LVRDLQYIASQARLLLPIHDPSPEVWDHIQQAIRQEKPEPPAAARK"
     gene            complement(927..1643)
                     /locus_tag="VHE33_17550"
     CDS             complement(927..1643)
                     /locus_tag="VHE33_17550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVT99312.1"
                     /translation="MSLTAQKTTNSRATGASSHATIARERFVLMAGPTQPRPANQHLR
                     RNPPIAGEQEAIDRAREGDGRAFETLYAMHKRRVYSLCLRMLGNVAEAEDLTQEAFLQ
                     LYRKIGTFRGDSAFSTWLHRLAVNVVLMHLRRKGLPQVSLEETLEPSQDDGPRKDIGA
                     RDLTLSGSIDRVTLERAIENLPPGYRLVFVLHDVEGYEHNEIAEMLDCSIGNSKSQLH
                     KARMKLRDLLRTGQRKEQIA"
     gene            complement(1815..2075)
                     /locus_tag="VHE33_17555"
     CDS             complement(1815..2075)
                     /locus_tag="VHE33_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HVT99313.1"
                     /translation="MAYVIAEPCIGTKDTACVDACPVDCIHPKKDEAKHAETDQLFID
                     PVECIDCGACVPVCPVSAIFAADDLPEKWASFQQKNAAFFGR"
     gene            complement(2191..3084)
                     /locus_tag="VHE33_17560"
     CDS             complement(2191..3084)
                     /locus_tag="VHE33_17560"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HVT99314.1"
                     /translation="MSQVPVHAWIPKEMDLAVRAVPIALEAGALIRGYFERGVATEYK
                     GDVDVVTEADRASEKLIAERLTAAFPGHGIYGEEGVRQRIESEYRWYVDPLDGTTNFS
                     HGFPVFCVSLGLEHRPAGKDGELIAGVIYDPTRDELFVAQKGLGAWLNGRRIHASKIK
                     HLAESLLSTGFPSRKRHISPNIHFYQEFTLRSHGVRRAGSAALDLAYTACGRVEGFWE
                     FHLNPWDTAAGALLVLEAGGAITRFDGAPFRLDSDEVLASNGLIHAELVQLFTDMFAG
                     RELHPIPSPAEFAAQRAARQR"
     gene            complement(3081..3383)
                     /locus_tag="VHE33_17565"
     CDS             complement(3081..3383)
                     /locus_tag="VHE33_17565"
                     /inference="COORDINATES: protein motif:HMM:NF028518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HVT99315.1"
                     /translation="MPPDQPSRRRHKNPPPGETGQEALYLRSLSDRQVPVHVKLHDGE
                     TVSGWIEYFDDRMIRITRDGKPNLFIYKNQIRTITEHGRRRPRNGSNDDGSAEDGQ"
     gene            complement(3409..3759)
                     /locus_tag="VHE33_17570"
     CDS             complement(3409..3759)
                     /locus_tag="VHE33_17570"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HVT99316.1"
                     /translation="MVRLLLKWLLQAFALLVTARLVPGFIVHSVTAALIAVLIIGLLN
                     VTLGLLLKLITLPLSILTLGLFFLVINAFILKLAGHVVPGFYVATWTAALIGAVVLAL
                     LHMLTSALLDKNEK"
     gene            complement(3914..4408)
                     /locus_tag="VHE33_17575"
     CDS             complement(3914..4408)
                     /locus_tag="VHE33_17575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99317.1"
                     /translation="MKNKFFAIALGGLLAFGANAALNAQDQASAQTPAPAQDQAAPPS
                     GQQQGQWGHRRGRHMDPDRQLAHLTKTLNLSADQQSQIKPILLDRQQKMQALWQDQSL
                     SRQDRRTKAIAIRQDTKARLEAALNDQQKQQFEELQAKMQGRRHNRMGGDNQAPPSSA
                     PQPQ"
     gene            complement(4515..5138)
                     /locus_tag="VHE33_17580"
     CDS             complement(4515..5138)
                     /locus_tag="VHE33_17580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99318.1"
                     /translation="MKSLLRLGSLLLLLSWSAGSFSAGVERRPGPFVAQCTGVTLHAS
                     YLADVGTHAGPGFAFHIENKTGKDIRLAQPVPSSAHWYARVGLRWLWRASAGRGGALV
                     NALVEKGPMFAFRPASPPEHAQYLTVPARGSEDWTVAMRDHPAIQYRPSCARCNYPGE
                     TDYRAIFAYAYLPNQDEHVPDLLRCGLRTAPVPMPPVALHDAALQTQ"
     gene            complement(5205..5690)
                     /locus_tag="VHE33_17585"
     CDS             complement(5205..5690)
                     /locus_tag="VHE33_17585"
                     /inference="COORDINATES: protein motif:HMM:NF041379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYCXC family (seleno)protein"
                     /protein_id="HVT99319.1"
                     /translation="MKRVLLSLLLGVAIVAAYAIPAGFDPASTVHAQWSDPAQDIPAY
                     HPLPPRKGEKLPPIMSGQQLTGQFFQQKWQVEVYKQAAQIPDVLYQIPCYCRCDRALG
                     HASLHSCFEGTHGAICATCAAEEAYAYKMTKQGKTPDQIRAGIERKEYEAIDLDALGR
                     S"
     gene            complement(6347..6625)
                     /locus_tag="VHE33_17590"
     CDS             complement(6347..6625)
                     /locus_tag="VHE33_17590"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HVT99320.1"
                     /translation="MPVLEDRVVAAKLKSVPSWSLERGELVRRCEFQNFIEAMHFVNS
                     VAEMAEGAGHHPDIDIRYNKVRLALISHDAGGLTERDFDLAAAIDSLG"
     gene            complement(6675..7781)
                     /locus_tag="VHE33_17595"
     CDS             complement(6675..7781)
                     /locus_tag="VHE33_17595"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HVT99321.1"
                     /translation="MASERILIVDDEPYVRTLMAAMLEKSNYTPVLASNGLEAIEHLE
                     HDPPYDLILSDIVMNGLDGIGLLERIRQVQPDTPMVMVTAIHDVGVAISAMRKGAYDY
                     LLKPFEKEQLLVTVRRALDYRRLVQQNSVYRHNLEQLVSARTEMLRQAMADLERSYDI
                     TLEALGDALDLKDAETEGHSRRVTAYTIALARHCGVEMAQTRIIARGAFLHDIGKMAI
                     PDAILLKPGKLDPEEQRIMREHCARGYQILRKIPFLEEAADIVYAHQEHYDGNGYPRN
                     LKGDEIPLGARIFAISDTVDAITSDRPYRKARSYDVARSEVEKCAGTQFDPNIVEVFL
                     GLPDSLWEDLRAEMTRHPRFSPLSFAGAIEDLKV"
     gene            8116..8652
                     /locus_tag="VHE33_17600"
     CDS             8116..8652
                     /locus_tag="VHE33_17600"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVT99322.1"
                     /translation="MLFEIDRQFAKITAREMAPQSLAEKQRDIYDSHRHRVFSVSYYM
                     TGSELEAEEILRGTFIRAFLREDEPDHAIIDTALLEQLRDQRVLVEETPLPAPTAGYL
                     PQRNNILRTELEEAIRILPSNERLVFLLMDVEGYAATRVAEMLNQSPSTVLRTALTAR
                     MRLRAELATMRDSNRQAA"
     gene            complement(8687..10084)
                     /gene="glmU"
                     /locus_tag="VHE33_17605"
     CDS             complement(8687..10084)
                     /gene="glmU"
                     /locus_tag="VHE33_17605"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HVT99323.1"
                     /translation="MRLGIIIMAAGKGTRLKSKRAKVLHEIGGRSLLAHVIASAAQVV
                     PPQDILVIVGHQAEAVQAATRETGVRFVVQTEQRGTGHAIQTAEPGTRDYDELIILSG
                     DVPLLRPETIVALRDFHLQERAAMTILTAEPDEPFGYGRILRKAPDAPEVDAIIEQKS
                     LRPGQENLREINSGIYAFRRESLFKHIGQLTANNSQKELYLTDMARLLHEAGERVLAR
                     KAAQPEEVLGANTIAEMMMLDRELRIATANRLMAAGVTIQQPETVMIDARVEVGADTV
                     IEPFVQLLGHTRVGSDCRIRSYSVIENSTVGDQVVILQSCVITDTQIDNGARIGPLAH
                     IRPGCHIGEGAHIGNFVEAKKTRLGKGSKANHLTYLGDAEIGSGVNIGAGTITCNYDG
                     VAKHQTVIGDKVFIGSDSALVAPLVIGEGAYIGAGSTITKDVPADALAVARGRQVTKE
                     GWAKNRRAQREARKA"
     gene            complement(10186..10569)
                     /locus_tag="VHE33_17610"
     CDS             complement(10186..10569)
                     /locus_tag="VHE33_17610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HVT99324.1"
                     /translation="MTSGASASVGKAMEWDHPTLRLLTITKEKPMSVQAIHPGEHLAE
                     ELSELGMSASSLARQLQVPTNRITEILGGRRAITGDTALRLAHFFGTTPEFWLNLQSL
                     YEIRMAQRKSGKAISGLPTLEERTA"
     gene            10941..>11342
                     /locus_tag="VHE33_17615"
     CDS             10941..>11342
                     /locus_tag="VHE33_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99325.1"
                     /translation="MPCLEMKVPALPHPVPATRLRALLEKPGAAVLRRNELAEAPGHD
                     DGELRNRIRVAAVLAVNLTDPPQPIETTEPKDEAEPAEEPHTVAEPEAEQQTGSFAKG
                     LEIWMSDGERAVCVLADADEIPGALAMFDAWL"
BASE COUNT         2110 a         3255 c         3766 g         2211 t
ORIGIN      
        1 gctcaactgg cggatgggcg tcatgcggtg gctccggcct tgctggaggc ggtggcgtgc
       61 cgatcgaggt cgccgagaat gcgctcgacg acggcctccg gcgcaaggtt cgccgtatcg
      121 atggtgagat gggcctgacg ataccagggg agacgcttat tccagcgttc gacgaggcgc
      181 gctcgatcag ccaggacagg acggacggga ccgcattcgt gtccagcgca gcgggcgatc
      241 atggtctcga ggggcgcttc gaggaagacg atgagggtgt cgtgaagaga ggcgaggaaa
      301 tcgcgggtgt cgctgcgctc cagcgcgccg cctcccacgg cgaggacaaa gggctcaccg
      361 ggcctgcagg ccagggcttc gcggatgacg gcggcttcgg catcgcggaa ggcggtctcg
      421 ccgcggcgct gaaaaatatc ggcgatagtc atggcctcgc gtgtttccac caggacgtcg
      481 gaatcgaaga agtcccagcc caggcgttcc gcgagcaggg ctccgacggt ggtcttcccc
      541 gcacccatga acccggtgag gatgatgcgc gtgggatggg ccggaaacga tgccggctgt
      601 ggcagagacc cgctcatgct ttgctgatta tatgaatggc cgggagaaca gcggcgctta
      661 tttgcgcgcc gcggccggag gctccggctt ctcctgacgg atggcctgct ggatgtggtc
      721 ccaaacctcg gggctggggt catgaatggg cagcaggagc cgggcctggg aagcgatgta
      781 ctggaggtcg cggaccagag cagcgcaatt ctcacacgac ttcagatgct cctggccgct
      841 gagatcagcc tgggtctcga aaagctcggg aagggcgtcc tgaaattcgg aacaagtcat
      901 ggttttgtcc atggcgcgga tcaccgtcac gcgatctgct ccttccgctg accggtgcgc
      961 agcaggtcgc gtagtttcat ccgggccttg tgcagctgcg acttgctgtt tccgatggag
     1021 caatcgagca tctccgcaat ttcattatgc tcgtagcctt cgacatcatg gagcacaaaa
     1081 accaggcggt atccgggtgg caaattctcg atggcgcgct ccagggtgac gcggtcgatg
     1141 gatccggaca gggtgagatc gcgggcgcca atatctttgc gcggtccgtc gtcctgggag
     1201 ggctcgaggg tctcttccag agagacctga ggcaatcctt tgcggcgcag gtgcatgagc
     1261 acgacgttca cggcgagccg gtgcagccag gtggagaatg ctgagtcgcc gcggaaagtg
     1321 ccgattttgc gatagagctg gaggaacgct tcctgggtga ggtcttcggc ctcggcgacg
     1381 ttgccgagca tacgcaggca gagcgagtag acgcgccgct tgtgcatggc ataaagggtt
     1441 tcaaacgcgc ggccgtcgcc ttcgcgcgct ctgtcgatcg cctcctgttc gccggcaatg
     1501 gggggattgc ggcggaggtg ctggtttgcc ggtctcggtt gtgtcggccc ggccatcagg
     1561 acaaaccttt cccgtgcaat tgttgcatgc gacgacgctc cagtggctct gctgttcgtg
     1621 gtcttctgcg cagtcaggct cataggcaaa acttttccgg ggagaaacgg tatgccatta
     1681 cgacgcagag gagaggtcag gggttgcttc gggcagcgga gagcggaagg gaaaggcgcg
     1741 ggcccaaagc gtcaagaaat gaaggctggg gacacacagc ggagggctgt gcgccccgag
     1801 gacactcgca gaattcagcg gccgaagaaa gcggcgttct tttgctggaa gctcgcccac
     1861 ttctcgggca gatcgtcggc agcaaagatg gccgagaccg gacaaaccgg aacacaggcg
     1921 ccgcaatcga tgcactcgac cgggtcgatg aaaagctggt cggtctcggc gtgcttcgct
     1981 tcgtcttttt tgggatggat gcagtcgacc ggacaggcat cgacgcacgc cgtgtccttg
     2041 gtgccgatgc agggttccgc aatcacgtaa gccatgaatt cctcttcagc agtcagagtg
     2101 acaacggatc attttagcag gggatcgcgg cgcggcaaca agcgccgcgc gcaggaaaaa
     2161 ttactttgga gtgggagcgg cagtctcgga tcagcgctgc cgcgcagcac gctgggcggc
     2221 gaattcggcg ggggacggaa tcggatgcag ctcgcggccg gcgaacatgt cggtgaagag
     2281 ctgaacgagt tccgcgtgaa tcaggccgtt ggacgcgagc acttcatcgc tgtcgagacg
     2341 gaagggcgcg ccgtcgaagc gggtgatggc gcccccggct tcaaggacca gcagcgctcc
     2401 ggcggcggta tcccaggggt tgagatggaa ctcccaaaag ccttcgacgc gtccgcaggc
     2461 ggtgtaggcg aggtcgagag cggccgatcc ggcgcggcgc acgccgtgcg agcgcagcgt
     2521 gaactcctgg taaaagtgga tgttggggct gatatgccgt ttgcggctgg ggaagcctgt
     2581 ggacagcagc gactcggcga ggtgtttgat tttcgacgca tgaatgcggc ggccattcag
     2641 ccaggctccc aggccttttt gcgcaacgaa caattcgtcg cgagtgggat cgtagatgac
     2701 gccggcgatc agctcgccgt ctttgcctgc ggggcggtgt tcgagcccga gggaaacgca
     2761 gaagactgga aaaccatgcg agaagttcgt ggttccgtcg agcggatcga cataccagcg
     2821 gtattcactc tcgatgcgct gccggacgcc ttcttcgccg taaatgccgt ggccggggaa
     2881 tgcggcggtg agccgttccg caatcagctt ttcggaggcg cgatcggcct cggtcacgac
     2941 atcgacgtca cccttatatt cggtggccac accgcgctcg aaataaccgc ggatgagcgc
     3001 gccggcttcg agtgcgatag gaacggcgcg cacagccagg tccatctctt tcgggatcca
     3061 tgcatggact ggaacctggc tcactggccg tcctcggcag acccatcgtc attggagccg
     3121 ttccgcggcc tgcggcggcc atgctcggtg atggtgcgaa tctgattctt gtaaatgaag
     3181 aggttcggct tgccgtcgcg ggtgatgcgg atcatgcggt cgtcgaaata ctcgatccag
     3241 ccggagacgg tctctccgtc atggagcttg acgtggacgg gaacctggcg atcgctcaac
     3301 gagcggaggt agagggcttc ctgtccagtt tcgcccgggg gtgggttttt gtgccggcgg
     3361 cgggagggtt gatcgggcgg catgaacgta ttgtagccgc cccgccgatc acttctcgtt
     3421 cttgtcgagc aaagcggagg tgagcatgtg caggagcgcg agcaccaccg cgccgatcag
     3481 agcggcggtc caggtggcga cgtagaagcc gggcacgacg tgtccggcca gcttgaggat
     3541 gaaggcattg atgacgagga agaacaggcc cagggtgagg atgctcaggg gcagcgtgat
     3601 gagcttaagc agcagaccga gggtgacgtt gagcaaaccg atgataagga cggcgatgag
     3661 cgccgcggta acactgtgga caataaagcc gggcaccagc cgggcggtga ccagcagagc
     3721 aaaggcctgc aacagccact taaggagcaa tcgcaccatg ggtcgacacc tctttcagtc
     3781 agcagccaat agccagtaaa tactacctgg gcgccatcgt ttctcaaagg ggagagattt
     3841 gtaacggaaa tcgttactga aggagaaggc cgccggcatg accggcggcc tgcagtcgca
     3901 cgcggcgaat ggcttactgg ggttgcggag cgctgctggg aggcgcctgg ttgtcgccgc
     3961 ccattctgtt atgccggcgg ccctgcatct tcgcctgcag ttcttcgaac tgctgcttct
     4021 gctgatcgtt cagagccgct tccagacggg ctttcgtgtc ctgccggatg gcgatggcct
     4081 tcgtgcggcg atcctggcgc gagagcgact gatcctgcca cagggcctgc attttctgct
     4141 ggcggtcgag caaaatcggt ttgatctggc tctgctgatc ggcggagaga ttgagcgtct
     4201 tcgtcaggtg ggcgagctgc cggtcgggat ccatgtggcg gccgcgacgg tgtccccact
     4261 gtccctgctg ctggccgctg ggcggtgctg cctggtcctg cgccggagcc ggggtttgtg
     4321 ccgaggcttg atcctgcgcg ttgagagcgg cgttcgcgcc aaacgcgagc agaccgccga
     4381 gtgcaattgc gaaaaatttg tttttcatgg tgccgggtac ttcctcctgt tgctgttttc
     4441 aacccgctgc gatggggcga gttccgcaga acagaagggg aaatttttgc tgaagactgc
     4501 gtctcaactg agatttattg cgtctgcagc gcggcgtcgt ggagcgcgac cggcggcatg
     4561 ggcacgggcg cagtgcgaag tccgcagcgc agcagatcgg gtacgtgctc gtcctgattc
     4621 ggcaggtagg cataagcgaa gatcgcccga tagtccgtct cgccgggata gttgcagcgc
     4681 gcgcagctgg gccggtactg aatcgcggga tggtcgcgca tggcaaccgt ccagtcctcg
     4741 gagccgcggg cgggcacggt cagatactgg gcgtgctcgg gcggagacgc gggacgaaac
     4801 gcgaacatcg gacccttctc caccagcgca ttgaccagcg caccgccacg accggcggag
     4861 gcgcgccaca gccagcgcaa acccacgcgc gcataccagt gcgcgctgga gggaacgggc
     4921 tgggcaaggc gaatgtcttt gccggttttg ttctcgatgt ggaaggcgaa tcctggaccg
     4981 gcatgggtcc ccacatcagc cagataactg gcgtggagcg tcacgccggt gcattgcgca
     5041 acgaaggggc cgggccgtcg ctcgacgccg gcggaaaacg atcctgcgct ccaggagagg
     5101 agaagaagga gagatccgag gcgcagaaga gacttcatga ctgagaatta tgccaagcga
     5161 tgagtgggtt tacagtccgc aggtcgagtc gttcccgatt gcgcttagct gcggccgagg
     5221 gcgtccagat cgatcgcttc gtactccttg cgttcgatgc cggcgcgaat ctgatccggc
     5281 gttttgccct gcttcgtcat cttgtaggcg taggcttctt cggcggcgca ggtggcgcag
     5341 atagcgccgt gcgttccttc gaagcagctg tgcaggctgg catggccgag cgcgcggtcg
     5401 cagcggcagt agcaggggat ctgatagagc acatcgggaa tctgcgctgc ctgtttgtag
     5461 acttccacct gccacttctg ctggaagaac tgcccggtga gctgctggcc ggacatgatg
     5521 ggaggcagct tctcgccctt gcgcggcggc aaaggatgat aggcaggaat atcctgcgct
     5581 ggatcggacc attgcgcatg cacggtgctg gccggatcga agccggcagg gatggcgtag
     5641 gcggcgacga tggccacgcc gagcaagagg ctgaggagga cgcgtttcac tggggactcc
     5701 agccttaatc gtaccaggag agaggattcc gggtggggag aaggggcgat gcaggggtca
     5761 aaagcaaaaa aaggcggcac gaacggtgat ttgtccgtgc cgggaggcca gtgacgcaac
     5821 ttgtgaactg ctgccagcgg cgaattctga gtcgccgttc cggcttaaaa ctcttcttca
     5881 aactctgatg ccttgcgctg gggcgatgga cgatttattt tgcccgacaa ggccgaaatc
     5941 agtactgctc gatctccaga atagtgtaac cctgaacccc caaatgtaca agcctgctac
     6001 acaatttaat atttttgaag gttcctaacc ttcccacttt gggacgttcc gcaccgtcca
     6061 tcccatgtcc aatcaaccac aaacgcccga aaaatcacaa tccggcctca ggatgaaggc
     6121 ctttctgaca ggcgcgatcg ttggcgcctc agccctgatt gggggtcttg ccgtcgtgct
     6181 ctggaaccgc aaggcacttg caggtctgcg ccagcacccc gaaccgggtc aaaatgctgg
     6241 tgcggaaccc gacgtcgacg gagagtaagg gctgcgagaa cgcctcgtgt ggcgaagttc
     6301 cgtgcgctgg ggttctgtgc gctggggttc tgtgcggggg cgcgcctcac ccaaggctgt
     6361 caatggctgc tgccaggtcg aaatcccgct ctgtcaggcc gccggcgtcg tgactgatga
     6421 gcgcgagacg caccttgtta tagcggatat cgatgtcggg atggtgcccg gcgccctcgg
     6481 ccatctcggc cacgctgttc acgaagtgca tggcttcgat gaagttctgg aactcgcagc
     6541 ggcgcaccag ctcgccccgc tcgaggctcc acgacggaac cgacttcagt tttgctgcga
     6601 cgactctgtc ttccaggaca ggcatggcct cttctccgca ctcgggatct caggcactcg
     6661 tcggtagatg agattcaaac tttcagatct tcgattgccc cagcaaatga taacggagag
     6721 aagcgcgggt ggcgggtcat ttcggcgcgc aaatcctccc aaaggctgtc gggcaggccg
     6781 aggaagacct cgacaatatt agggtcgaac tgggtaccgg cacatttttc gacttcggaa
     6841 cgggccacgt cgtaggaacg ggctttgcgg taggggcggt cggaggtgat ggcgtcgacg
     6901 gtgtcggaga tggcaaaaat ccgcgcgccg agggggatct cgtcgccttt caggttgcgc
     6961 gggtagccgt tgccgtcgta atgctcctgg tgggcataga cgatgtcggc ggcctcttcg
     7021 agaaagggga tcttgcgcag gatctggtag ccgcgggcgc agtgctcgcg catgatgcgc
     7081 tgctcttcgg ggtcgagctt gccgggcttg agcaggatgg cgtcgggaat ggccatcttt
     7141 ccaatgtcgt gaaggaatgc gccgcgggcg atgatgcgcg tttgcgccat ctccacaccg
     7201 caatggcggg ccagggcgat ggtgtaggcg gtcacgcggc gggagtgccc ttcggtctcg
     7261 gcatccttga ggtcgagcgc gtcgcccaga gcctcgaggg tgatgtcgta ggaacgctcc
     7321 aggtcggcca tggcctggcg gagcatttca gtacgagcgg aaacgagctg ctccagattg
     7381 tggcgataga cagagttctg ctggacgagg cggcgatagt ccagagcgcg gcggacggtg
     7441 accagcagct gctctttttc gaaaggcttg agcagatagt cgtaggcgcc tttgcgcatc
     7501 gcggagatgg cgacgccaac gtcgtggatg gcggtcacca tgaccatggg cgtgtcgggc
     7561 tgaacctggc gaatgcgctc gagcagcccg atgccgtcga ggccgttcat gacgatgtcg
     7621 gagaggatca ggtcgtaggg agggtcgtgt tcgagatgct cgatggcttc gaggccgttg
     7681 gaggccagga ccggggtgta gttggatttt tcgagcatag cggccatcag cgtgcgaacg
     7741 tacggctcgt cgtccacgat caggatccgc tctgacgcca ctgccccctc cagcctttgg
     7801 ctcgatacac ctgctgagtt ttgttggaga cccgtaagaa ttccgctcta tcgtatcctg
     7861 cgaatcgtcg acaatgcgtg caattcccgg aacatagaac caggaggacc atttcggatc
     7921 actcctgttg cgcagttcct gcgacgggaa tcgcagcccg agtcgaggcc tacaacagcg
     7981 gtgtcaccac tcggttcccc aggattctat tttgcgccat cacctgccct tggctctctc
     8041 ccccaaacgg ggcaaacgtg catctaacgt tcgtgttaat cttctcttgc cggatcccag
     8101 agccggctct cacgcatgtt gttcgaaatc gaccgacagt tcgcaaaaat taccgcccgt
     8161 gaaatggcgc cgcagagtct ggctgaaaag cagcgagaca tctacgacag ccatcgccat
     8221 cgggttttct cggtctccta ctacatgacg ggcagcgaac tggaagcgga ggagatcctg
     8281 cggggcacgt tcatccgcgc ttttctgcgc gaggatgagc cggatcacgc gatcatcgac
     8341 acagcgttgc tggagcaact gcgcgaccag cgcgttctgg tggaagaaac gcctctccct
     8401 gcacccacgg ccggctatct gccgcagcgc aacaatattc tgcgcacgga gctggaggaa
     8461 gccatccgca tcctgccttc gaacgagcgc ctggttttcc tgttgatgga tgtggaaggc
     8521 tacgcggcca cccgggtcgc ggagatgctc aaccagagcc cgtccacggt gctgcgcact
     8581 gcgctgaccg cgagaatgcg gctgcgcgcc gagctggcga ccatgcgcga cagcaatcgc
     8641 caggccgcct gattcccgtt tagtcccccg ctcttgtgtc gctgcgttac gctttgcgcg
     8701 cttcgcgctg cgcgcggcga ttcttcgccc agccttcttt cgtcacctga cggccacgcg
     8761 ccacggccag ggcatcggcg ggcacgtctt tcgtgatcgt agagccggct ccgatatagg
     8821 ccccctcgcc gatgaccagc ggcgccacca gcgccgagtc gctgccgatg aagaccttgt
     8881 cgccaatcac ggtctggtgc ttcgcgacgc cgtcgtaatt gcaggtgatg gtgccggcgc
     8941 cgatgttgac cccgctgccg atctccgcgt cgcccagata cgtcaggtgg tttgctttgg
     9001 agcctttccc gaggcgggtc ttcttggctt cgacgaagtt gccgatgtga gcgccctcgc
     9061 cgatgtggca gccggggcga atgtgggcca gcgggccaat gcgggcgccg ttgtcgatct
     9121 gcgtatcggt gatgacgcac gactgcagga tcaccacctg gtcgccgacg gtggagtttt
     9181 cgattaccga ataagagcgg atgcggcagt cgctgccgac gcgggtgtgt ccgagcaact
     9241 gcacgaaggg ctcaatgacg gtgtccgcac cgacctcgac acgcgcgtcg atcatgaccg
     9301 tctccggttg ctgaatggtg acgccggcgg ccatgagccg attggccgtg gcgatgcgca
     9361 gttcgcggtc gagcatcatc atctcggcaa tggtgttggc tcccaggact tcttcaggct
     9421 gcgccgcttt gcgtgccagc acgcgctcgc cggcctcgtg cagcagccgg gccatatcgg
     9481 tgaggtacag ctctttctgg gaattgttcg cggtgagctg cccgatgtgt ttgaacaggg
     9541 attcgcggcg gaaagcgtaa atgccggagt tgatctcgcg caggttttcc tggccaggcc
     9601 ggagagattt ttgttcgatg atggcatcga cctccggcgc atccggggcc ttgcgaagga
     9661 tgcgtccgta accaaaaggc tcgtcgggct cggcggtcag gatggtcatc gcggcccgct
     9721 cctggagatg aaaatcgcgc agggcgacga tggtctcggg gcgcagcaga gggacgtctc
     9781 cggagagaat gatcagctcg tcgtagtcgc gggtgcccgg ctcggcggtc tggatggcgt
     9841 gacccgtgcc tcgctgctcg gtctgcacga cgaagcgcac gccggtctcg cgcgtggcgg
     9901 cctgtacggc ctcagcctga tgtccgacga tgaccaggat gtcctggggc ggcacgacct
     9961 gggcggcgga ggcaatcacg tgcgccagca acgatcgtcc gccgatctca tggagcacct
    10021 tggcgcgttt ggatttgagc cgggtgccct tgcccgcggc catgatgatg attccgagtc
    10081 gcatggattg tcagaactcc ttcagcgagc gtgcagatgc tgcagacccg cagggtaacg
    10141 atcattctct caaacgcaca tccgcagcag gctgccaacg ctgcgtcagg ctgtgcgttc
    10201 ttccagagtc ggtaacccgc tgatcgcctt gccggatttt cgctgtgcca tccggatttc
    10261 atacaggctc tggagattca gccagaactc aggcgttgtg ccaaagaagt gtgccaggcg
    10321 cagagctgta tcgccagtga tcgctctccg cccacccaag atttctgtga tgcgattggt
    10381 cggaacctga agctgacgag caaggctcga agcactcatg ccaagctcgc tcagctcttc
    10441 agccaggtgt tctccgggat gaattgcctg cacactcatc ggcttctcct tagtgatagt
    10501 caacaacctc aacgtcggat ggtcccactc catcgctttc ccaacggaag cagatgcgcc
    10561 actggtcatt cacgcgaatg ctgtattcgc cctttctgtc tcccagcaga gattccaacc
    10621 ggttccccgg cagcgcagca agatcgcgca atgtggtggc tgcttccaga cggtccagtt
    10681 tcattctcaa cgcacgctca aatcccgaga atgccttgac ccgccggccg tcagcgaact
    10741 ccctggtccg cttgtcgcgg tagctaacga tcacaagaga tcgtaacttc tgttatgcgt
    10801 tacgtcaagc gtaacgggat tcatctctca tggcacccgc tcgtcaaagc ggcaatcatt
    10861 ctgatacctg gtctcaaatt tccttaatcg tgaggcttgc actcgtatac tttcgagcag
    10921 cgacccgcgc gaggagacgc atgccctgtc tggaaatgaa agtgccggct ctgccgcacc
    10981 ctgtcccggc cacgcggctc cgcgcgcttc tggaaaaacc gggcgccgca gtgctccggc
    11041 gcaacgagct ggcggaggcg ccgggacacg acgatgggga gctgcgcaac cgaatccgcg
    11101 tcgcagcggt tctggccgtg aacctcaccg atcctccgca gccgatcgag acgactgagc
    11161 caaaggacga agcggaaccg gcagaagaac ctcacaccgt agccgagcct gaggccgagc
    11221 agcagacggg cagcttcgcc aaggggctgg agatctggat gagtgacggc gagcgcgcgg
    11281 tgtgtgtgct ggccgatgca gacgagattc cgggcgcgct ggcgatgttc gatgcgtggc
    11341 tg
//
LOCUS       DASZYN010000680         1799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1255484, whole genome shotgun sequence.
ACCESSION   DASZYN010000680 DASZYN010000000
VERSION     DASZYN010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255484"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1799
                     /locus_tag="VHE33_17620"
     CDS             <1..>1799
                     /locus_tag="VHE33_17620"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99326.1"
                     /translation="GRINKAWGLALRKRFCRGFNFELQAAATDNGINICLAEQHSFPL
                     SDVFHFLSTETVGELLEQASIASPIFKTRWRWDASRSLQLLRFQKGRKVAPQIQRIRS
                     DDLLASVFPQAAACFENIEGDIQIPDHPLVKEVMRDVLQEAMDLEGLKAVIEAMHEGR
                     IRCVAVDTTTPSAFAHELINAKPYAFLDDAGLEERRARAVQLRGVLPDAELGEAGRLS
                     QDAIDEIRQEIWPDIRDEHELHDLLCGLVVVPVETLGHPGARDWDVFLSRLVQSGRVL
                     TAKRQEMAYVVAAERAQWLRSLWPDVEFSVEMLKIPGEVPQNSDILRKTLQGWLQISG
                     PVTSTAISEQLGLDPAAVFQQMVHLESTGTILRGVFEGAHGRESVPDTEIEWCERRIL
                     QRIHRRTLATLRKQIEPVTPAVYMRFLLDWQHLGEQRQLSGEQGLLEALRGLEGFEAP
                     AAEWERSILPQRVAGYDPRWLDALCLTGAVGWGRISPHPAFADASSGGPRRVVPTSMA
                     PVTFFIREEALWMDLCLSQRQIPEANLNACLSELANRLRSLLTERGAMFAGDLIRMLG
                     APADEVHRALWELVAAGVVHADGFDSLRLLIDPR"
BASE COUNT          321 a          529 c          591 g          358 t
ORIGIN      
        1 ggccgcatca acaaggcgtg gggactggca ttgcgcaagc gtttctgccg cggcttcaat
       61 ttcgagctgc aggccgccgc gaccgacaac ggcatcaaca tctgcctggc ggagcagcac
      121 agctttcccc tcagcgacgt ctttcacttc ctttcgactg agacggtcgg cgagctgctg
      181 gagcaggcat cgattgcttc gccgatcttc aagacgcgct ggcgctggga tgcctcgcgg
      241 agtttgcaac tgttacggtt ccaaaaagga cgcaaggtcg ctccgcagat ccagcgcatt
      301 cgaagcgacg atcttctggc cagcgttttc ccgcaggcag cggcgtgctt cgagaacatt
      361 gagggcgaca tccagattcc cgaccatccg ctggtgaagg aagtgatgcg cgatgtgttg
      421 caggaggcga tggatctcga gggcctgaaa gctgtcatcg aggccatgca cgagggacgc
      481 attcgttgtg ttgctgtgga cacgacgacg ccttcggctt tcgcccatga actgatcaac
      541 gccaagccct acgctttcct cgacgatgcc ggactggagg agcgccgcgc gcgcgccgtt
      601 cagttgcgag gtgttctgcc cgatgcggag ctgggcgaag ccgggagact gtcccaggat
      661 gcgatcgacg aaatccggca ggaaatctgg cccgatatcc gcgacgagca cgagcttcat
      721 gacctgctgt gcggtctggt ggtcgtacca gtcgagaccc ttggtcatcc tggggcgcgg
      781 gattgggacg tttttctgag ccgcctggta cagtcgggcc gcgtcctgac cgcgaaacga
      841 caggagatgg cgtatgttgt cgcggccgaa cgggctcaat ggcttaggag cctttggccg
      901 gatgtggaat tctctgtgga aatgttgaaa atccctggag aagtccccca gaacagtgac
      961 attttgcgga aaacgttaca gggatggtta caaatttcag gtccggtgac ctcgacagcg
     1021 atctccgagc agcttggcct cgatccggcc gctgtttttc agcagatggt ccatcttgaa
     1081 tcgaccggaa cgatcctgcg cggtgtcttc gaaggagcgc acggccggga atctgtgcct
     1141 gacactgaga ttgaatggtg cgagcgccgc atcctgcagc gcatccaccg gcgaacgctg
     1201 gcgacgctgc gcaagcagat cgaaccggtc acgcccgcgg tctacatgcg cttcctgctc
     1261 gactggcagc acctgggcga acagcgtcag ctcagtggcg agcagggact gctggaagcg
     1321 ctgcgcggtc tggaaggatt cgaggcgccg gcagccgagt gggagcgatc gatcctgccg
     1381 cagcgcgtgg ccggctacga tccgcgctgg ctcgatgctc tttgcctcac gggtgctgtg
     1441 ggctggggcc gtatctcgcc gcatccggca tttgccgatg cgagcagcgg tggcccgcgc
     1501 cgcgtggtgc ccacgagcat ggcgccggtg acgtttttca tccgcgaaga ggcgctgtgg
     1561 atggatttgt gcctgtcaca gcgacaaatt ccggaggcaa acctcaacgc ctgcctgagc
     1621 gaactggcca atcgcctgcg gtcgctcctc accgagcgcg gggcgatgtt tgccggcgat
     1681 ttgatccgca tgctcggcgc tcccgcagat gaggttcatc gcgctttgtg ggagctggtc
     1741 gcggcaggcg tggttcatgc ggacggcttc gacagcctgc gcctgctcat cgatccgcg
//
LOCUS       DASZYN010000681         4187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_99475, whole genome shotgun sequence.
ACCESSION   DASZYN010000681 DASZYN010000000
VERSION     DASZYN010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99475"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VHE33_17625"
     CDS             complement(<1..393)
                     /locus_tag="VHE33_17625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99327.1"
                     /translation="MNPTQIPSPSRPAPPPEQKLSTAPALIDPAGPDVSLETSEAMFD
                     IGVALNACGYDRGLDDSEPVRKTVRDEVNAAIRSSADAADDRDKLCAFIDQHRTDEPS
                     RNLAQYVSLALYLTPPPQLTPSVEEQDLP"
     gene            663..2435
                     /gene="ggt"
                     /locus_tag="VHE33_17630"
     CDS             663..2435
                     /gene="ggt"
                     /locus_tag="VHE33_17630"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HVT99328.1"
                     /translation="MVSRLLPACLVTALALPALAQQAKPVHDGAPDDAGRKSIAYLDE
                     QPARARHAMVVTVHHLATDAGVTILRAGGNAVDAAVAVGFALAVVHPVAGNIGGGGFM
                     LIHQHDGRNLFIDYREKAPLASTTNMYLDAQGNIIPNASIVGYRAIGVPGAVAGLVHA
                     EKRYGKLTLAKVMAPAIHLAADGFALSDEEAAELHDKILTGFPASRTIFQRDGNFYKT
                     GDVFRQPELAETLRKIARDPDDFYRGAMAKQIAKAIQKGGGLITAEDMARYTVKEREP
                     VTGTYHGYEIVSAPPPSSGGVALIEMLNILDGYRLRPLGDRTAPEMHLITEAFRRAYM
                     DRSNYLGDPDYVKIPVEKLISKTYGDAWRKSIETDQASASASLQRPSGFLPPAPTMAD
                     VRQEAADTTHYSVMDGEGDAVAVTTTLNGGFGSGVTVPGLGFLLNDEMDDFASKQGVP
                     NMFGLIQGPANAIAPGKRPLSSMTPTIILKDGKVAMVLGSPGGGRIITTVANIFLSVA
                     DEGLNIQQAVDAPRFHHQYLPDVLFLEPGFSSTSLEGLKAMGYNVRVGGHWSDGECIA
                     VDPKTGELEGGQDHRHHYGEAAGY"
     gene            complement(2495..3067)
                     /locus_tag="VHE33_17635"
     CDS             complement(2495..3067)
                     /locus_tag="VHE33_17635"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HVT99329.1"
                     /translation="MARCTALTVKPIESQIILVRGQRVLIDSDLAALYGVEVRAINQA
                     VKRNKSRFPPDFVFRLTAKDLRTLRSQTVISNSNRGGRRYLPYVFTEHGAIMAASVLN
                     SPRAVEVSIFVVRAFVHLRKTFAAHKALAAKLSELEQRLETHDTVISKIIHTIRSLTT
                     PPERPRRQIGFRPEMVSRPKMLKAGTQVMS"
     gene            3349..>4187
                     /gene="gnd"
                     /locus_tag="VHE33_17640"
     CDS             3349..>4187
                     /gene="gnd"
                     /locus_tag="VHE33_17640"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF006765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HVT99330.1"
                     /translation="MPEAICDIGLIGLAVMGQNLVLNMNDHGFKVAVFNRTVSKVDDF
                     VNNEAKGTQVVGAHTIEEFVGLLKSPKRVMLMVKAGDTVDQMVDHVLPYLSPGDIVID
                     GGNSLFTDSNRRTKDLAAKGILFIGTGVSGGEEGARFGPSIMPGGNPDAWPHVKDIFQ
                     SIAAKVDENGQATNSSGKGTPCCDWVGDAGAGHYVKMVHNGIEYGDMQLICEAYDLLK
                     HTLNLTPDELSAVFVDWNKGELDSYLIEISSIIFAKKDEDGTPIVDKILDTAGQKGTG
                     KWTV"
BASE COUNT          853 a         1198 c         1341 g          795 t
ORIGIN      
        1 cggcaggtcc tgctcttcga cgctcggcgt cagttgcgga ggcggcgtga ggtagagcgc
       61 gagggacaca tattgtgcga gattgcgcga aggctcgtcg gtgcgatgct gatcgatgaa
      121 ggcgcagagc ttgtcgcgat catcggccgc atccgctgag gagcgaatgg ccgcattgac
      181 ctcgtcgcga accgttttgc gcacgggctc tgaatcatca agaccgcggt cgtaaccgca
      241 cgcgttgagg gcgacgccga tgtcaaacat ggcctcgctg gtttcgaggc tgacgtcggg
      301 tccggcggga tcgatgaggg ccggcgcggt cgaaagcttc tgctcgggag gcggcgccgg
      361 acggctgggc gaggggattt gcgtggggtt catccccgga acctggctgg aactggagcc
      421 cggggtttgc tgagccagac aagaagcagc ggagacgcat cccgcgagaa ggatcagcag
      481 gggaaagtgg cgggaatacg acgacataga gattggacgg ttgaactgcc cttcagtcta
      541 tgcgcgggtg aaaggagggt caatccgggg atcggtcatc ggtgctcgga tggccgccct
      601 ttcccaggct tacaattctc gaattgagtt gcgccccgcc tatgaaaatt tcccggcgac
      661 cgttggtttc tcgtcttctt cctgcgtgtt tggtcacagc gcttgcgctc ccagctctgg
      721 cccagcaggc aaagcctgtt cacgacgggg ccccggacga cgccggccgt aagtctatcg
      781 cgtacctgga cgagcagccg gcgcgcgctc gccacgccat ggtggtgacg gtccatcacc
      841 tggccaccga cgcgggcgtg accattctgc gagcgggcgg caacgccgtc gacgccgccg
      901 tcgccgtcgg attcgcgctc gcggtcgtgc atcccgtcgc cgggaacatc ggcggcggcg
      961 gattcatgct gatccaccag cacgacgggc gtaatctctt catcgactat cgcgagaaag
     1021 cgccgctggc ctcgaccacc aatatgtatc tcgatgcgca gggaaacatc attcccaacg
     1081 ccagcatcgt gggatatcgc gcaatcggcg tcccgggtgc agtagcgggc ctggtccacg
     1141 ccgagaaaag atacggcaag ctgacgctgg ccaaagtcat ggcgccggca attcacctgg
     1201 ccgccgatgg atttgcgctg agcgatgagg aggctgccga acttcacgac aagatcctga
     1261 ccgggtttcc cgcgtcgcga acgatctttc agagggacgg gaacttctat aagacggggg
     1321 acgttttccg gcagccggag ttggcggaga cgctgcgcaa aatcgcccgg gatccggatg
     1381 atttctatcg cggcgcgatg gcgaagcaga tcgccaaggc tatccaaaag ggcggcggac
     1441 tgatcactgc cgaggacatg gcacgttaca ccgtgaagga gcgcgagccg gtgaccggca
     1501 cgtatcacgg atacgagatc gtgagcgctc cgccaccgtc atccggaggc gtggcgctga
     1561 tcgagatgct gaacatcctc gacggctacc gtctgcgccc gctcggcgac cgcaccgcac
     1621 cggagatgca cctgatcacg gaagcatttc gtcgggcgta tatggatcgc tcgaactatc
     1681 tcggcgatcc cgactacgtg aagattccgg ttgagaagct gatcagcaag acgtatggcg
     1741 atgcgtggcg aaagtccatc gagaccgatc aggccagcgc gtcggcgtcg ctccagcgtc
     1801 cgtcgggatt cctgccgccg gcacccacca tggccgatgt acgccaggag gctgcggaca
     1861 caacgcacta ctcggtgatg gacggcgagg gcgatgccgt ggccgtgacc acgactctca
     1921 acggcgggtt cggatcgggc gtcacggtgc cgggtttggg attcctgctg aacgacgaga
     1981 tggacgactt cgcctcgaag cagggcgtac cgaacatgtt cggcctcatt cagggccccg
     2041 cgaatgcgat tgcgccgggc aagcggccgc tctcgagcat gacgccgacc atcattctga
     2101 aggatggcaa ggtcgcgatg gtgctgggat cgccaggggg cggccggatc atcacgacgg
     2161 tggcgaatat tttcctgagc gtcgctgacg aggggctcaa tatccagcag gccgtggacg
     2221 ctccgcgatt tcaccaccag tatttgccgg acgtgctgtt cctggagccg ggattttctt
     2281 caacttcact ggagggtctg aaagcgatgg gttacaacgt ccgagtcggc ggacactggt
     2341 ccgacggcga gtgcatcgca gtcgatccga agacaggaga gctggagggc ggccaggatc
     2401 atcggcatca ttacggagaa gcggccgggt attgatgggc atgagaccga ttcctgcata
     2461 aaggcgcgtc ttagcgcggc gccgatgcag gcattcagga catcacctgc gtgccggcct
     2521 tcagcatctt tggcctcgag accatctccg gacgaaagcc gatctggcgg cgcggcctct
     2581 ccggcggtgt cgtgagcgag cggatcgtat ggatgatctt gctgattacg gtgtcgtgcg
     2641 tttccagccg ctgctccagt tccgacaact tcgccgcgag cgccttatgc gcagcgaacg
     2701 tcttgcgcag gtgtacaaaa gcacgcacca caaaaatgct gacctcaacc gcgcgcgggg
     2761 aattgagcac gctggcagcc atgatggcac cgtgttcagt aaagacgtaa ggcaggtaac
     2821 ggcggccgcc ccggttcgag tttgagatca cagtttgtga tctcaaagtc cgaaggtcct
     2881 tcgccgtaag ccgaaaaaca aaatccgggg ggaagcggct cttgtttcgc ttgactgcct
     2941 ggttgatggc tctgacttcg acaccgtaga gtgccgcaag gtcggagtcg atgagaacgc
     3001 gctggccgcg aacgagaatg atctgactct cgatcggttt tactgtgagg gcggtgcacc
     3061 tggccatttt gaccccttat tttgagatca caaattgtga tctcaaagaa aaacaggatt
     3121 gcggaaaagc gatggatgcg aggccgcagg aggccgcctg ttcagcccca tcgcaaaaag
     3181 gatcccatgc gaatcgcgcc atgaatcgaa aagtgtgggt tttctctgag cggtggtcac
     3241 gttaccttgc gcctccggac ggcgtcttct tgccccgtgc tccggcctct ggtttatcct
     3301 gctcgaaaag cgcactattt tgcagggtat tcctgctaag ggagcgacat gccagaagcg
     3361 atctgtgata tcggcctgat cggccttgcg gtcatgggcc agaaccttgt gctgaacatg
     3421 aacgatcacg gtttcaaggt ggccgtcttc aaccgcaccg tgtcgaaagt cgacgacttt
     3481 gtgaacaacg aagcgaaggg cacgcaggtg gtcggcgcgc acacgatcga agagtttgtc
     3541 gggctgctca agagcccgaa gcgcgtcatg ctgatggtca aggccggcga taccgtcgat
     3601 cagatggtcg atcatgtgct gccctatctg tcccctggtg acatcgtcat cgatggtggt
     3661 aactccctgt tcaccgattc caatcggcgc acgaaagacc tcgccgcgaa aggcatcctc
     3721 ttcatcggca ccggcgtctc gggcggcgaa gaaggcgcgc gtttcggacc gtcgatcatg
     3781 cccggcggca atcccgacgc atggccgcat gtgaaagaca tcttccagtc cattgccgcc
     3841 aaggtcgacg aaaacgggca ggcaacgaat tccagcggca agggcactcc ctgttgcgac
     3901 tgggtgggcg atgcgggcgc aggccactac gtcaagatgg tccacaacgg catcgagtac
     3961 ggcgatatgc aactgatctg cgaagcctac gacctgctga agcacaccct gaatctcact
     4021 ccggatgagt tgtccgctgt cttcgtcgac tggaacaagg gcgaactgga cagttacctg
     4081 atcgagattt cgtcgatcat cttcgcgaag aaagacgaag acggtacacc catcgtcgat
     4141 aagattctcg acactgccgg tcaaaaggga acagggaagt ggacggt
//
LOCUS       DASZYN010000682        16950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_232774, whole genome shotgun sequence.
ACCESSION   DASZYN010000682 DASZYN010000000
VERSION     DASZYN010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 16950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16950
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232774"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="VHE33_17645"
     CDS             <1..1217
                     /locus_tag="VHE33_17645"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVT99331.1"
                     /translation="NALKGEVAFTGERRRMSVRNLLVVAQVAMALILLSLTGLFLRSL
                     EHASRIDIGFRTRGLLLLSVDPRLNGYTPAKTAAFLRELRERARALPGVDAAVCTDVP
                     LLSGGNRSEGFTLSGHSAKETPVTADLYMVTPGYFDAMGTPLLAGQDFSDEPADGPRV
                     AVVNKAFADRRFGGTNPIGQRVEDAAGTYQIIGVAGNAKSRTVGEATRPILYRSLDQS
                     IASDPSMMGYTLVVHTAGNPATLVNAVRAQVYALDPAMAIYNVETMEDHVRAAYLLPR
                     LAAMLFGVFGCIGMVLATTGLYGLMSFAVSRRTREIGIRMALGARRGVVLRMVLRRAM
                     LLTLVAFALGWPAAWLLARIAASFLYGISPHDTVTFAAVPVVLAIAALVAVWVPARRA
                     AAVNPTEALRAE"
     gene            complement(1233..2405)
                     /gene="dusB"
                     /locus_tag="VHE33_17650"
     CDS             complement(1233..2405)
                     /gene="dusB"
                     /locus_tag="VHE33_17650"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HVT99332.1"
                     /translation="MKKSWDNPIEHAMPQGTRVPASLQIGNVTVAPATVLAPMAGVTD
                     TVFRRFIRNASVFSSAPDHPSSIEDQVTNQQSGCGLIMTEFTSADGLSRMRETKRKRY
                     LTFYDDEHPIAAQIFGSNPATLAEAARIVEDTGFDMVDLNLGCPSKRVVSCNGGSGLL
                     RDLPLIGEIFCTVRAAVTIPFTVKFRLGWNDNHIICVPLAKLAEDSGLNAVSLHARTR
                     EQGYTGQARWHHIAEVKDAVKIPVIGNGDVRTPEDACALIAKTGCDAVMIGRAAPANP
                     WIFRQIAQYTATGGYDAPTADDRHRMIRTYFEMLIAEANPEAPGKMKQFASWFTHGVP
                     GGAALRKSIYESRSGTAILDAVDRFFSGEGRAVQADSESDTAALTVDSGSSLLVCD"
     gene            complement(2471..2710)
                     /gene="rpmE"
                     /locus_tag="VHE33_17655"
     CDS             complement(2471..2710)
                     /gene="rpmE"
                     /locus_tag="VHE33_17655"
                     /inference="COORDINATES: protein motif:HMM:NF001809.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HVT99333.1"
                     /translation="MPKEGIHPNYHEIRVVCACGNSFATKSTHKGDIHAEICSNCHPF
                     FTGKQKLVDTAGRVERFRRKYAKTEAKPEPAGAAK"
     gene            2908..3966
                     /locus_tag="VHE33_17660"
     CDS             2908..3966
                     /locus_tag="VHE33_17660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HVT99334.1"
                     /translation="MRAVVLKQRPAPGTLPDVLRIESIAEPVPGPREAVVQVLASTIN
                     IDDIHVIEGTFYGGIPLGPRPRPDRPVTPGSDLAGIVIATGKDVRSVRVGDAVFGVQM
                     PLRRQGAWAEQCAVDERWLTRKPDPVSFEMAAACGVSGLVALSALKALRMRAGMRVVV
                     VGATGGIGAIVVQLAARAGVEVIGVCGPANVERARQLGCSLVLDYHRGPWDRALHQEG
                     MSRVDRVFDAVGGEDTEQMGRRVLGRDGIFVTVVGPERFIGDRALGWRGILAILARVS
                     ARMLRSYVRGPRYVLTGPGPGGGNALAEVAAAASAGVLPPIDSTVAFEQNAMREALER
                     AAAHRNHGRIVIQVGAAG"
     gene            complement(3979..4494)
                     /locus_tag="VHE33_17665"
     CDS             complement(3979..4494)
                     /locus_tag="VHE33_17665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99335.1"
                     /translation="MAMRAATVMCLALTAAIPCLAQSGQSDSQTLQAILAEIRAIHQE
                     VKVTQTSQILLTELQVQQTVVNRATQRVDDAQSRLNDIKLAQKENAGEQARIKERLEQ
                     PISDPNEAQALTEHAEELKRQAATMATIEQERTTNLQSAQQQLKDAQDSLDEIQSQLN
                     AIVKQMTPARN"
     gene            complement(4559..5485)
                     /locus_tag="VHE33_17670"
     CDS             complement(4559..5485)
                     /locus_tag="VHE33_17670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99336.1"
                     /translation="MAANSLLIRCRRLLAHPRMRWVHDPQLWVELFATANLGILAADI
                     YIAHSVNQFGVKAEYVPLYFSIAAAALLAVAIALAWLAGMRRTWSITGTIVGWTAILT
                     GLAGVLYHLDCRFFAERTLQSITYAAPFAAPLSYTGLGFLLLLNRSVAPRTAEWARWV
                     IFLALGGFFGNFVLSLTDHAENGFFLHTEWIPVLSSALAVGFLAVPLLARVTRRYLDW
                     CMLVMFAQALVGVLGFWFHLRANLVEPGHSLFDKLVNGAPPMAPLLFPNLVALAWIGL
                     WALIPHVPEDSTGHSWLGATWLWAHPDQPAGD"
     gene            complement(5472..6707)
                     /locus_tag="VHE33_17675"
     CDS             complement(5472..6707)
                     /locus_tag="VHE33_17675"
                     /inference="COORDINATES: protein motif:HMM:NF024839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HVT99337.1"
                     /translation="MSFRSVFIAVVIGFSLIVAAFLINMARPRVETQQPTADFVRASG
                     KCAECHARLQYSVVHEFEMSTHARKGVNCLDCHQPAAGQDKLDHHGFVIAKHLTAGNC
                     RSCHEQEYQEFLRSRHAAPSWAAVFGEKSLTPEQVSFSEQYQPGGTKRPPHPFVAAEG
                     NSAMTSGCAQCHAIGKPNDDGTIGTCTACHSRHTTSVELARLPSTCGQCHMGPDHSQM
                     EIYNESKHGVMFAAQRNLLNLKAPPKQLTTRDMFVPTCATCHMSGINGLKITHDPSDR
                     LSWFLAAEVTTKRPNYLRAQAAMKQVCEQCHTAPTIDRVYAQGEQVILTTNARVQHAK
                     DIVTSLRAAGLVKGPPFTNPIDFLYFDLWHYDGRTSKHGAFMGGADFVQWHGNYPMLQ
                     KTVELEHMSAELRKQHGGE"
     gene            7033..8031
                     /locus_tag="VHE33_17680"
     CDS             7033..8031
                     /locus_tag="VHE33_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HVT99338.1"
                     /translation="MLSIEVTRECPLHCPGCYAYGSNHLGGEVTLRELSDKRGDDLVA
                     GVLGLVDQHKPMHVTLVGGEPMVRHRELSQILPVLAGRNIFTMVVSSGIIPFPMEWMA
                     LPGFVATVSVDGLPEHHNVRRHPATYEKILANVEGRKVNIHLTVTAQMLERPGYFEEY
                     ARFWSERPEVRSIWVSFYTPQRGEQSAEMLSQEQRVQVAKELAHLHTIYPKLLTNEGY
                     TQALLHPPASPKECSFSRLSKNYSADLKTHVEPCVFGGDPDCSQCGCVASVASHWVSG
                     MKVLGPLTPGHIMRGSLGIGNAMARLQDLATPSWRQREVPSAKSGELVQITDERVA"
     gene            complement(8125..8400)
                     /locus_tag="VHE33_17685"
     CDS             complement(8125..8400)
                     /locus_tag="VHE33_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99339.1"
                     /translation="MTRERWAQIGITIQFLIVVRTLGEIFRLRHVHGANFSAAMAMPY
                     VGGDLIAACFCWVSVTLYFFRRYLFAAWITLAAVATLLIYKITVIGF"
     gene            8462..9445
                     /locus_tag="VHE33_17690"
     CDS             8462..9445
                     /locus_tag="VHE33_17690"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HVT99340.1"
                     /translation="MFRFPALLIVMILPFAVKTDPGATRQILVHAHRGGRAYRPENTI
                     PAFEYGISAGADVLELDLAVTKDNVLVVSHSPFLTQPSAASLSNDPAAAAAIGAALAN
                     ERHCDGPPLPPGTYIHSLTLAQLRQYDCGSHTLPAFSTQVAVPHTPIPTFDEVLDLAP
                     RGSFQFNVETKIFPNRPEITPTPEAFVAMIDQAVKRHHLQSRVILQSFDFRTLRAMRK
                     IDPAIRLSALFGQSRYDKMMGISDEDKSFAHIAEAAQLRKGDFFSPDVSLATPEEVKW
                     AHEHGMLVAPYTANTPAAWQKLADAKVDAIITDDPAGLLHWLRAQSPPLHP"
     gene            9663..11207
                     /locus_tag="VHE33_17695"
     CDS             9663..11207
                     /locus_tag="VHE33_17695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVT99341.1"
                     /translation="MTRSESAAQLFGKPSVLTEGANNFLAAIIASSDDAIISKDLNGI
                     ISSWNAGAQRIFGYEAPEIVGQSILRLIPPDLQHEEEFILAKIRAGERIEHFETTRMR
                     KNGERFPISVTISPIKDNSGRIIGASKIARDITDRRRADESRLRLAAIVDSADDAIIS
                     KDLNGIITSWNNGARRLFGYEPEEMIGQSILRLFPEDLRHEEAEILRKLRAGEKIEHY
                     ETTRISKTGERCEVSITISPIRNHLGEVIGASKIARDVSDRKKVERLAIEAEKIATTG
                     RMAAAIAHEINNPLGSVLNLIFLARQEGISPQEVQSYLATAEGELERVSHIARQTLGY
                     YRDTGSPSEIHLHGLVENVLSVYRNRVVAHNITLEASFNDLRKIRVRPGEIVQVLSNV
                     VSNAIDAMPQGGRLSIRTTQTTKGEQEGLYLIIGDTGKGIRREHIGRVFEPFFTTKGN
                     LGTGIGLWVAKQLVERHGGQISISSSTERDDSGTVVTIFLPFSRAQNMTLQPSEPESR
                     SESGAS"
     gene            11194..11661
                     /locus_tag="VHE33_17700"
     CDS             11194..11661
                     /locus_tag="VHE33_17700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT99342.1"
                     /translation="MELPDFVVFPADRVSGGVSGFPLTESESEKTVARQPRVLVVDDE
                     RLLADTTAAILRRTGFNAKTAYDAFAALELSGSFHPDYLLTDIMMPGMNGVELAIAMT
                     KMHPATKILLISGQAGISDILAGSREKGYEFPLLAKPVHPETLVEGLRNLNNG"
     gene            complement(11639..12220)
                     /locus_tag="VHE33_17705"
     CDS             complement(11639..12220)
                     /locus_tag="VHE33_17705"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HVT99343.1"
                     /translation="MSFIATPDWSTLPVPEDDGAARHLPGLRLPPIPLVATDGLSVNL
                     ESLAGRVVLFIYPRTGVPDQPNPPGWDQIPGARGCTTQCNAFRDAFAELRALGVDHLY
                     GLSTQSTADQREMAERQRLPFPILSDEAFALTRALRLPTFEASGMTLLKRLTLIAQDA
                     VIEQVFYPVFPPDQNAAEVAAWLRGNLSRCSGS"
     gene            12603..12797
                     /locus_tag="VHE33_17710"
     CDS             12603..12797
                     /locus_tag="VHE33_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99344.1"
                     /translation="MELTLSVEEHEFLLKTLEQRHRELLREIAHTDHREFKHGLRMDE
                     KLLDSLVDRLRRTAVQEIRG"
     gene            12881..13618
                     /locus_tag="VHE33_17715"
     CDS             12881..13618
                     /locus_tag="VHE33_17715"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 family protein"
                     /protein_id="HVT99345.1"
                     /translation="MLLLIWLVLCLMAIALLLLFPALWRRDTYKQYFGWRSVVCPENQ
                     QPAVVCMDARHAAVTGMHGHPDVRLSNCTRWPERSQCGQTCLSQAIHADRYGPDVAVG
                     TKAIHHLPVLLAAFAAWCVGAVWHSQYLFRPRLVDAVGLTRAQVKQMVWWISPQLLTL
                     AACVLFAYGVAWLLAVSHRKGVLPGVLMAVLLCGAVVAASWYGIGRLPHALLVIEAGY
                     IVLAVLIVGTIVGGLFDRLALPHKVVP"
     gene            complement(13680..14669)
                     /locus_tag="VHE33_17720"
     CDS             complement(13680..14669)
                     /locus_tag="VHE33_17720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99346.1"
                     /translation="MKVRVFYHDKCFDGACSASLFSRFHQERIHPDATYEFCGLVHRA
                     GALFNEADFIGDENAIVDFKYSPSPKITWWFDHHLSAFLTPEDHQDYRSGIADGRYRG
                     HRFYDPEYVSCTSFLAHIASTQFGFDPTPVAELVRWADIVDGALYESPQLAVEMPAPA
                     MKLTLIIESTQDNAFIPRLIPTLTRMPLQQVLEQPWVASLLPPLFERHQRAIDLIRSR
                     AESKEGTIYFDITDHPLEGYNKFIPYYLHPEATYSVGLSKSSFRTKVAVGSNPWTRTA
                     PEKMINLATICERYGGGGHARVGAISFPPDREDEARKAAAEIVAELRAANPSP"
     gene            14847..15464
                     /locus_tag="VHE33_17725"
     CDS             14847..15464
                     /locus_tag="VHE33_17725"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HVT99347.1"
                     /translation="MAQLVQYFLAKWGYLALATALFGESAGLPLPGETVLLFSSFVAH
                     KTGELRIGLVILIGTTAAICGDNLGYLAGRHFGPRLLKWLKRTFNMEEDVAAASALIR
                     RHGGATVFWARYVIGLRAIAGPVAGALNMEWKRFLLYNALGGFCWVSSISLTGYFFAA
                     RVQSLTGFIEKVSWAIAGTVFAVGYLLWRRMKKQQKLRTRQRQAA"
     gene            complement(15476..15952)
                     /locus_tag="VHE33_17730"
     CDS             complement(15476..15952)
                     /locus_tag="VHE33_17730"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVT99348.1"
                     /translation="MISRRNVVAGLMTMPAVLTAMAEAVQDAKPAPAPENKEILGPHV
                     FEWDQLQFHKTATGEVASLCKQPTATVDQLEMHVSKLNPGTASHPPHRHVNEELIIIR
                     EGDCETLSDGKWVKVGPGSVVFNASNSLHGFRNVGTTMAVYHVINWSPNKEKLSAR"
     gene            complement(16087..>16950)
                     /locus_tag="VHE33_17735"
     CDS             complement(16087..>16950)
                     /locus_tag="VHE33_17735"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99349.1"
                     /translation="QIGSQQRSTIQFRYAAELVRNGRIGDLQRVEIGLPGDPAGGDKT
                     PMPVPDGFNYEMWLGETPYVYYTVDRVHPPKGYDRPGWLRCRQFGAGMITGWGAHHID
                     SAHWGMNTEYTGPIEIWGQAQFPDHGLWDVHGPFKTEANYANGVHMSISGDYPNGIKF
                     YGSKGWIFVSRGNEQVTKSDPVAKLNDNTALASSDPAIIKSTIGPDEIHLYESKDQHG
                     NWLDCIVTRQQPISPAEMGHRACSTCLIHDMAMVLRRKLYWDPVNERFHNDDEANSML
                     ARPQRAPYRLS"
BASE COUNT         3155 a         4866 c         5687 g         3242 t
ORIGIN      
        1 ccaatgcgct gaagggagaa gtcgccttca cgggcgagag gcgccgcatg agtgttcgca
       61 acctgctggt ggtcgcgcag gtggcgatgg cgctgatcct gctctcgctg acagggcttt
      121 ttctgcgcag cctggagcac gcgtcgcgga tcgacattgg ttttcggaca cgcggactgc
      181 tgctgctctc ggtcgatccg cggctgaatg gatatacgcc ggcaaagacg gccgcattcc
      241 tcagggaatt gcgggagcgc gccagggcgc tgccgggcgt ggacgcagcg gtgtgcaccg
      301 atgtgccgct gctttcgggc ggcaatcgca gcgagggctt tacgcttagc ggtcactccg
      361 cgaaagagac tcccgtgacg gccgatctct atatggtgac gccgggctat tttgacgcga
      421 tgggaacgcc gctgctggca ggacaggatt tcagcgatga gccagccgac gggccgcgtg
      481 tcgcggtggt caacaaggca ttcgcggatc ggcgcttcgg gggaaccaat ccaattgggc
      541 agcgcgtcga ggacgcggcc gggacctatc agatcatcgg cgtggcgggg aatgcgaagt
      601 cgcgcacggt gggggaagcg actcgaccga ttctttaccg ttcgctcgat cagagcatcg
      661 cgagcgatcc ttcgatgatg ggctacaccc tagtggtgca cacggcgggg aatccggcga
      721 cgctggtcaa cgcggtacgg gctcaggtgt acgcactcga cccggcgatg gccatctata
      781 acgtggaaac catggaggac catgtccgcg cggcctatct cttgccgcga ttggcggcga
      841 tgctgtttgg agtcttcgga tgcatcggga tggtgctggc gacgaccggg ctctacggcc
      901 tgatgagctt cgccgtgagc cggcggacgc gggagattgg cattcgcatg gccctcggcg
      961 cgcggcgagg cgtggtgctc cggatggttc tgcgccgggc catgctgctg acgctggttg
     1021 cttttgcgct gggctggcct gcggcatggc tgctggcgag gattgccgcg tctttccttt
     1081 acggcatttc accgcacgac acggtgacgt ttgccgccgt gccggtggtc ctggccatcg
     1141 ctgcgctggt tgctgtgtgg gtgccggcgc ggagagcggc tgcggtgaat ccaaccgaag
     1201 cattgcgggc ggagtgacgc gaggcgttga ttttagtcgc agaccagcag cgaggatcct
     1261 gaatcaaccg tgagggcggc ggtgtccgat tctgagtccg cctggacggc gcgtccttcc
     1321 ccgctgaaga accgatcgac ggcgtccagg atggccgtgc cggagcggga ttcgtagatg
     1381 gacttgcgca gcgcggctcc gccgggaacg ccatgcgtaa accaggaggc gaactgcttc
     1441 atctttcctg gagcttccgg attggcttcg gcgatcagca tttcgaagta agtccggatc
     1501 atgcggtggc gatcgtccgc ggtgggcgcg tcgtagccgc cggtggcggt gtactgcgcg
     1561 atctggcgga agatccacgg attggcgggc gcggcgcggc cgatcatcac ggcatcgcag
     1621 ccggttttcg cgatgagggc gcacgcgtcc tcgggggtgc gcacgtcgcc gttgccgatg
     1681 accgggatct tcacggcatc tttgacctcg gcgatatggt gccagcgcgc ctggccggta
     1741 tatccctgct cgcgcgtgcg ggcatggagc gataccgcgt tcaggccgga gtcttcggcg
     1801 agcttcgcga gcggaacgca gatgatgtgg ttgtcgttcc agccgaggcg gaacttgacg
     1861 gtgaatggaa tggtgacggc agcgcgcacg gtgcagaaga tttcgccgat cagcggcagg
     1921 tcgcgcagca ggccgctgcc gccgttgcag gacacgacgc gtttcgaagg gcagccgagg
     1981 ttgaggtcga ccatgtcgaa gccggtgtcc tcgacgatgc gggcggcttc ggcgagggtc
     2041 gccgggttcg atccgaagat ctgagcggca atggggtgtt catcgtcata gaaggtcagg
     2101 tagcgtttgc gcttggtttc gcgcatccgg gagaggccgt cggccgaggt gaactcggtc
     2161 atgatgaggc cgcagccgga ctgttggttg gtgacctggt cttcgatcga cgagggatgg
     2221 tcgggcgcag atgaaaagac actcgcgttg cggatgaagc ggcggaagac ggtgtcggtg
     2281 acgccggcca tgggggccaa cacggtggca ggggcgacag tgacgttgcc gatctggagg
     2341 gacgcaggca cacgagtgcc ctggggcata gcgtgctcga tcggattgtc ccaggacttc
     2401 ttcatgcgcc gcacttaaaa caatggcccc cgccgcggcg gaggccttgt ctttccagag
     2461 ccgccaatcc ctacttcgcg gctcctgccg gctccggctt ggcttcggtt ttagcgtact
     2521 tgcggcggaa gcgctcaacg cggcccgcgg tatcgaccag cttttgtttg ccggtgaaga
     2581 acgggtggca gttcgagcag atttccgcgt ggatgtcgcc cttgtgcgtg gacttggtgg
     2641 caaaactgtt gccgcacgcg caaacgacgc gaatctcgtg atagttgggg tggattccct
     2701 ctttaggcat gatgctgttc cctaagacct ttcctgcaat ccttttattt taccgtgaac
     2761 gggccgattg gccaacgcgg acgtatggga tgcgcgaggg caggcatggg attcagggcg
     2821 gggcggaccg gcgatggagg ggaaaaaaac tacggccctg caccctcctc agcgaacagc
     2881 aaccgcttcc cttctagaat tgaggtggtg cgagccgtag ttttgaagca gcgcccggcc
     2941 ccgggaaccc ttcccgacgt tctccggatc gaatccatcg ccgagccggt tccgggtccc
     3001 cgcgaggcgg tggtccaggt gctggcgtcc acgatcaaca tcgacgacat ccacgttatc
     3061 gaggggacct tctacggagg gattccgctc ggtccgcgcc cccggccgga ccggccggtg
     3121 acgccgggga gcgatctggc gggcatcgtc attgcaacgg gaaaagacgt gcgctcggtg
     3181 cgggtgggag acgcggtttt cggcgtgcag atgccgttgc gccggcaagg agcgtgggct
     3241 gaacaatgcg ccgtcgacga gcgatggctc acgcgcaaac ccgatcctgt ctcgttcgag
     3301 atggcggccg cctgcggagt ctccgggctg gtggcattgt ccgcgctgaa ggctctgcgg
     3361 atgcgagccg gcatgcgcgt ggtggtcgtt ggggcgacgg gcggaatcgg ggcgatcgtg
     3421 gtccagttgg cggcgcgcgc cggcgtcgaa gtcatcggcg tttgcgggcc ggccaacgtc
     3481 gaacgtgccc gccagctcgg ttgctcgttg gtgctggact accaccgtgg tccatgggat
     3541 cgggcgttgc accaggaggg catgagccga gtcgatcgcg ttttcgatgc cgtcggcggc
     3601 gaagatacgg agcagatggg gcggcgcgtt ttgggacgtg acggcatctt tgtcaccgtc
     3661 gtgggcccgg agcgcttcat cggcgaccgt gctctgggct ggcgcggcat cctcgcgatt
     3721 ctggcgcggg tcagtgcgcg gatgctgcgt tcctatgtgc gcggacctcg ctatgtgctg
     3781 accgggccgg ggccgggcgg aggcaacgca ctggcggaag tggcggccgc tgcgtcggcg
     3841 ggcgtgcttc ccccgatcga ttcgacggtt gcgtttgagc agaacgcaat gcgggaagcg
     3901 ctcgaacgtg cggccgccca ccgcaaccat ggccgcatcg tgattcaggt cggcgctgcg
     3961 ggctgaggct tcgcgaattt agttccgagc cggcgtcatc tgcttcacga tggcgttgag
     4021 ctgactctgg atctcatcga gcgagtcctg ggcgtccttg agctgttgct gggcggactg
     4081 gagattggtg gtgcgctcct gttcgatcgt agccatcgtg gcggcctggc gcttcagttc
     4141 ctctgcgtgc tcggtaaggg cttgggcctc attcggatcg gaaatcggct gctcgagtct
     4201 ttctttaatc cgcgcctgtt cgccggcgtt ctctttctgg gccagcttaa tgtcattcaa
     4261 ccgggactga gcgtcatcga cgcgctgggt ggcgcgattg acgacggtct gctggacctg
     4321 cagttcggtg aggagaatct gactggtctg ggtgactttg acttcctggt ggatggcgcg
     4381 aatctcggcg aggatagcct gcagggtttg agaatcggac tggccggact gcgccagaca
     4441 tggaattgcc gctgtgagtg ccaggcacat aaccgtcgct gcgcgcatcg ccatcttgct
     4501 cctccgctgt tgctgagtat tctcgcggcg caagcgggcc gggggcaacg gatatttgtc
     4561 agtcgcccgc gggctggtcg gggtgagccc acagccaggt ggcgccgagc caggagtgtc
     4621 ccgtggaatc ttccggaacg tgggggatca gagcccagag gccgatccac gcgagagcaa
     4681 caagattggg aaagagcagc ggagccatgg gcggcgcacc gttgacgagc ttgtcgaaga
     4741 gtgagtggcc gggctcaacg aggttcgcgc gcaaatggaa ccagaagccg agcaccccga
     4801 cgagcgcctg ggcgaacatg acgagcatgc accagtccag atagcggcgc gtgaccctgg
     4861 cgagcagggg cacagcaagg aagccaacgg cgagggcgga ggagaggacc gggatccatt
     4921 cggtgtgcag gaagaatccg ttctcggcgt gatcggtgag cgaaagaacg aagttgccga
     4981 aaaacccgcc aagggcaagg aagataaccc agcgggccca ttccgcggtg cgcggagcaa
     5041 cgctgcggtt gagcaacagc aggaagccga ggccggtata ggagagcggc gcggcgaagg
     5101 gcgcggcgta ggtgatgctc tgaagagtgc gctcggcgaa gaagcggcaa tcgaggtggt
     5161 agagaacgcc ggcgaggccg gtgaggatcg cagtccaccc cacaattgtg ccggtaatac
     5221 tccaggtacg gcgcatgccc gccagccagg cgagcgcgat agcgacagca aggagcgcgg
     5281 cggcggcgat ggagaaatac aacgggacat attcggcttt gacgccgaat tgattcacgg
     5341 aatgcgcaat gtagatgtcg gcggcgagaa tgccgagatt ggcggtggcg aagagctcga
     5401 cccagagctg gggatcgtgc acccagcgca tgcgcggatg ggcgagcagg cggcggcagc
     5461 ggatgagaag actattcgcc gccatgctgc ttcctcagtt cggcggacat gtgctccagc
     5521 tccaccgttt tctggagcat cgggtagttg ccgtgccact gcacgaagtc ggccccaccc
     5581 atgaaggctc cgtgcttcga cgtgcggccg tcgtagtgcc agagatcgaa gtagagaaag
     5641 tcgatgggat tggtgaaggg cgggcctttg accaaaccgg cggcgcgcag gctggtgacg
     5701 atgtctttgg catgctgaac ccgggcgttg gtggtgagga tgacctgctc gccctgagcg
     5761 taaacgcgat caatggtggg cgcggtgtgg cactgctcgc agacctgctt catggcggcc
     5821 tgtgcgcgca ggtagttggg ccgcttcgta gtcacttctg cggcgaggaa ccagctgagt
     5881 cggtccgacg gatcgtgcgt gattttgagg ccattgatgc cggacatgtg gcaggtggcg
     5941 caggtgggca cgaacatgtc gcgagtagtg agctgtttcg gcggcgcttt cagattaagt
     6001 aaattccgct gcgcggcgaa catcacgccg tgcttcgact cgttgtaaat ctccatctgc
     6061 gaatgatcgg ggcccatgtg gcactggccg caggtggaag gcaggcgggc cagttcaaca
     6121 gacgtcgtgt ggcgcgagtg gcatgccgtg caggtgccga tggtgccatc gtcgttgggc
     6181 ttgccgatgg cgtggcactg agcgcatccg ctggtcatcg cggagttgcc ttcagcggcg
     6241 acgaaaggat gcggcgggcg tttagtgccg ccaggctggt actgctcgga gaaggagacc
     6301 tgctcgggcg tgagactctt ttcgccaaag acggcggccc aggagggtgc ggcgtggcgg
     6361 ctgcggagga actcctggta ttcctgctca tggcaggagc ggcagttccc tgccgtgagg
     6421 tgtttggcga tgacgaagcc gtggtggtcg agcttgtcct ggccggcggc cggctgatgg
     6481 cagtcgaggc agttgacgcc tttgcgggcg tgggtggaca tctcgaattc gtgcacgaca
     6541 gagtactgca gccgggcgtg gcactccgca catttaccgg aggcgcggac gaagtcggcc
     6601 gtgggctgct gcgtttcgac gcgggggcgt gccatgttga tcaggaaggc ggccacgatg
     6661 agcgaaaaac cgatgacgac agcgataaag acggagcgga acgacatgcc ggtcggcctc
     6721 ccggacgaga ttttgggggg aatcgtgctg ggtccccaaa aggagtgcct cgccactgta
     6781 gcagcggggc ggaaacatcc gctaccagga tgagccggag aggagtgcag cgaatcagtc
     6841 cggggcgggc gctccctcgc cgcagcggcg atttgtccgg gcaagggtgg aagtgtgaat
     6901 cacgggcccc gcgggaacgt ctaagctagg agtaccagct gggcccatcc tcaccatccc
     6961 caaggggtga ccaatttgcg gaccgtcgac attttgaaat cgtggagcaa ggtcctgcgg
     7021 ggccatactc cgatgctgtc catcgaagtg acgcgcgagt gcccgctgca ttgcccggga
     7081 tgttatgcct acgggtcgaa ccacctgggc ggcgaagtga cgctgcgcga gctgagcgac
     7141 aaacgcggcg acgacctggt ggccggcgtg ctggggctgg tggatcagca caaaccgatg
     7201 cacgtcaccc tggtgggcgg cgagcccatg gttcggcatc gcgagctgag ccagattctg
     7261 cccgtgctgg ctggacgcaa catcttcacc atggtggtat cgagcgggat cattcccttc
     7321 cccatggagt ggatggcgct gcccggtttt gtggcgacgg tgtcggtcga cggactgccg
     7381 gagcaccaca acgttcggcg tcacccggca acctacgaga agatcctggc caacgtggaa
     7441 ggccggaagg tgaatatcca cctgacggtc actgcgcaaa tgctggagcg ccccgggtat
     7501 ttcgaggagt atgcgcggtt ctggagcgaa cggcctgagg tgcgatcgat ctgggtcagc
     7561 ttctacacgc cccagcgcgg cgagcagagc gcggagatgc tgagccagga gcagcgggtg
     7621 caggtggcga aggaactggc gcatctgcat acgatttatc ccaaactgct caccaatgag
     7681 ggatacacgc aggctcttct gcatccgccg gcatcaccga aggagtgctc cttctcgcgg
     7741 ctttccaaga actactccgc ggatttgaaa acgcatgtgg agccctgcgt gttcggaggc
     7801 gatccggact gcagccagtg cggatgcgtg gccagcgtag cctcacactg ggtgagcggg
     7861 atgaaggtcc tggggccgct gacgccggga cacatcatgc gcggctcgct gggcatcgga
     7921 aacgcgatgg cgcggctgca ggatctggcg acgccgtcgt ggcggcagcg ggaagtgccg
     7981 tcggcgaaga gcggggagct ggtgcagatc acagacgagc gcgtggcgta gaaagcggcc
     8041 gggacatgag gattggctca gggatgcccg taccaccttg ctgacctgac gctccggact
     8101 cgaatccttc cggcctggct cgacttaaaa gccgatcacg gtgattttgt agatcaaaag
     8161 ggtggcgacg gccgccagag taatccacgc agcaaacaga tagcgccgga agaagtacaa
     8221 cgtgacgctg acccagcaga aacacgccgc gatcaaatcg ccgcccacat agggcatggc
     8281 cattgccgct gagaagttgg cgccgtgtac atgcctgagc cggaatatct cacccagcgt
     8341 ccgaaccacg atcaggaatt gaatcgtaat gccgatctga gcccagcgtt ctcgcgtcac
     8401 ggcaagaatt gtagcctgag cctttcatca atacgtcatc gtcccaatgg atactaatgt
     8461 catgttcaga tttccggccc ttttgatcgt catgatcctt ccgtttgccg tgaagacgga
     8521 ccccggcgca acgcgccaaa ttctcgtcca cgcccatcgc ggtgggcgag cttaccgccc
     8581 ggaaaacacg attcccgcgt tcgagtacgg catctcggcg ggagccgacg tgctcgaact
     8641 cgaccttgcc gtgaccaaag acaatgttct ggtcgtttcg cattcaccgt ttctcaccca
     8701 gcccagcgca gcgagtctct cgaacgatcc ggccgccgcc gctgcaatcg gagcggcgct
     8761 cgccaacgag cggcactgcg acgggccgcc ccttccgccg ggtacctaca tccattcgct
     8821 gacgctggct cagctcaggc aatacgattg cggttcgcac acgctgccgg cgttttcgac
     8881 ccaggtcgcc gtaccgcaca cgccaattcc gaccttcgac gaggttctgg acctggcccc
     8941 gcgcggctcc tttcagttca atgtcgaaac caagatcttt cccaatcggc cggagattac
     9001 tccgacgcct gaagctttcg tcgcgatgat cgatcaggct gtgaagcgcc accacctgca
     9061 gtcgcgggta atcctgcaga gcttcgactt ccgcaccctg cgcgccatgc ggaagatcga
     9121 tcccgccata cgcctgtcgg cgctctttgg gcagagccgc tatgacaaga tgatggggat
     9181 cagcgacgag gacaagagct tcgcccacat cgcggaggcc gcacagcttc gcaagggcga
     9241 cttttttagc ccggatgtct ccctggccac gccggaagag gtgaagtggg ctcacgaaca
     9301 cggcatgctg gtagcgccct acaccgcgaa cactccggca gcctggcaga agctggccga
     9361 tgcgaaggtc gatgcgatca tcacggatga tccggccggt ctgctccact ggctgcgcgc
     9421 gcaaagcccg cctctgcatc cctgagcccc ttgcggaagc cccgagtccc gacgacggcc
     9481 ggccgatgcc gatctgagcc acgcgttcct gtgttcgtga agaattgccg cggcgacgcc
     9541 tcttcccagg agggggcaaa aaaccatcgg atcgaaatct gcatctttct tcgtggagca
     9601 gtgtaggtgc catggcggcg gttcccgcag ccatggcatc ttcacggcga atgagaccac
     9661 cgttgacaag atcagaatcc gcagctcaac tcttcggaaa acccagcgta ctcacggagg
     9721 gagcgaataa tttccttgcc gccatcattg cgtcgtcgga cgacgcgatc atcagcaagg
     9781 atctcaacgg cattatcagc agctggaacg ctggagccca acgcattttc ggctacgagg
     9841 cgcccgagat cgtcggtcaa tccattctgc gcctgattcc ccccgacctg cagcacgaag
     9901 aagagttcat cctggccaag attcgcgccg gagaacgcat cgagcatttc gaaaccacgc
     9961 gcatgcgaaa gaacggagag cgctttccca tctccgtgac catctcgccc atcaaggaca
    10021 acagcggacg aatcatcggc gcttccaaaa ttgcgcgcga tatcacggat cgccgccgcg
    10081 cggacgagag ccgccttcgc ctcgcggcca tcgtggactc agccgatgac gccatcatca
    10141 gcaaggatct caacggcatt atcaccagct ggaacaacgg agcgcgccgt ttatttggct
    10201 atgaaccgga agagatgatc ggccagtcca tcctccgcct gtttccggaa gatctccgcc
    10261 atgaagaagc ggaaattctt cgcaagctga gggccggaga gaaaatcgaa cactacgaga
    10321 ccaccagaat cagcaagacc ggcgaacgct gtgaggtttc catcacgatc tctccgatcc
    10381 gcaaccacct tggagaagtg attggagcgt cgaagatcgc gcgcgacgtc tcagaccgta
    10441 aaaaggtgga gcgtctggcc atcgaagccg agaagatcgc aaccacgggc cggatggccg
    10501 ccgccatcgc tcacgagatt aataatccgc tgggctcggt gctgaacctg atctttcttg
    10561 cccgccagga gggcatttcg ccgcaggagg ttcagagtta ccttgccacg gcggaaggag
    10621 agctggaacg ggtctcgcac atcgcccggc aaacactcgg ctactatcgc gatactggat
    10681 ccccctccga gatccatctc cacggcctgg tcgaaaacgt tctgtcggtg taccggaacc
    10741 gcgtggtggc gcataacatc acgctcgagg ccagcttcaa cgatctgaga aagatcaggg
    10801 tgcgccccgg cgaaatcgtt caggtgctct ccaacgtggt ttccaacgcg atcgatgcca
    10861 tgccccaggg gggcaggttg tcgatcagga caacccaaac cacaaaggga gagcaggagg
    10921 gtctttacct catcatcgga gacaccggca aaggcatccg ccgtgaacat atcggcaggg
    10981 ttttcgagcc ctttttcacc accaagggca atcttggaac cgggatcggg ctatgggtcg
    11041 ccaaacagct cgtggaacgc cacggcggtc agatctcgat ttccagcagc accgagcgcg
    11101 acgacagcgg caccgtcgtg actatctttc ttcccttcag ccgcgcgcag aatatgacgc
    11161 tgcaaccatc cgaaccggaa agcaggtctg agagtggagc ttcctgattt cgtcgttttt
    11221 ccagcagatc gggtttccgg aggagtttcc ggattcccat taaccgagtc cgaaagcgag
    11281 aaaacggttg cgaggcagcc cagggttctg gtcgtcgatg atgaaaggct tctggcagat
    11341 accaccgccg ccattttgcg tcgcaccgga ttcaacgcaa agaccgccta tgacgccttt
    11401 gcagctctgg aattgagcgg atccttccat cccgattacc tgctgaccga catcatgatg
    11461 ccgggcatga acggcgtgga gctggcaatc gccatgacga aaatgcaccc cgcgaccaaa
    11521 atcctgctga tctccgggca ggccgggatt tcggacatcc ttgccggcag ccgggagaag
    11581 ggatatgaat tcccgctgct ggccaaaccg gtccatcccg aaaccctggt cgaagggctc
    11641 aggaacctga acaacggctg agatttccgc gcagccaggc ggcgacttcg gcggcgttct
    11701 ggtcaggcgg aaagacgggg taaaagacct gctcgatgac ggcgtcctgc gcaataaggg
    11761 tgaggcgctt gaggagcgtc ataccgctgg cctcgaaggt tggcaggcgc agggcgcggg
    11821 tgagcgcgaa cgcttcgtcc gacagaatgg gaaacggcag gcgctgacgt tcggccatct
    11881 cacgctggtc cgcggtcgat tgcgtagaga gaccgtaaag gtgatcgacg cccagagcgc
    11941 gcagctcggc gaaggcgtcg cggaaggcgt tgcattgcgt cgtgcatccg cgtgcgccgg
    12001 ggatctgatc ccatccaggc ggattgggct gatcgggaac ccccgtccgg ggataaatga
    12061 acaggacgac gcgcccggca agcgactcga ggttcacgct caaaccatcg gtggcgacga
    12121 gcgggatcgg cggaaggcgc aggccaggca aatggcgcgc agctccgtcg tcttccggaa
    12181 cgggcagcgt ggaccagtca ggcgtagcga tgaaagacat gggaacaggg tacagggagg
    12241 cgctttctgc cagacccctg cagagcccgt gctccccggc aaaggtgctc cggtggatag
    12301 tccgccttgc gcatcggcca tgaacgctcg ctcggctaag tgtcagtcca ttggctcgtt
    12361 tcgtgcgatc aacaatcccc gcctggtgtg ggcgccgtca cagctgagca gaggtaccat
    12421 cgtgagcgcg ggatgaaggc gcgcgggccg cgcctgcgtg gagtgtttgt ttgcaggatc
    12481 ggacgcagtc cctgctgcgt ccgcgccagc gtgggagttt gagctcgcga gatacaggat
    12541 gctcaccggc tcgcaagccg ccgtaaattc cgcggatgca gcgaggctgc gaggagggag
    12601 tcatggaact gacactcagt gttgaggagc atgagttcct gctaaaaacc ctggagcaac
    12661 gccatcgcga gttgctgaga gaaattgcgc acacggatca ccgcgagttc aagcatggac
    12721 tgcgaatgga cgagaagctt ctcgattccc tggtggaccg cttgcggagg acagcggtgc
    12781 aggaaattcg cgggtagagc acaactccgg tacgggtgac gggaatcact gaaggaattt
    12841 cgccaggaga gtagaaccct ggtcagaaag aaggtgggcc atgctgctcc tgatctggct
    12901 tgtgctctgc cttatggcga ttgcgctgct tctgctcttc cccgcactct ggagacgcga
    12961 cacctacaag caatattttg gctggcgatc cgtcgtctgc cccgaaaacc agcagccggc
    13021 cgtcgtctgt atggatgccc ggcatgcggc agtgaccggc atgcacggcc atccggatgt
    13081 tcgcttgtcg aactgcacgc gctggcctga acgctcgcag tgcggccaaa cctgcctctc
    13141 gcaggcaata cacgctgatc gctacgggcc cgatgtcgcg gtgggaacaa aagcgattca
    13201 tcacctgccg gttttgctgg cggcgtttgc ggcctggtgc gtgggagccg tttggcattc
    13261 gcagtatttg ttccggccgc ggttggtcga cgctgtggga ttgacgcgcg cgcaggtgaa
    13321 gcagatggtg tggtggattt cgccgcagct gctgaccctg gctgcctgtg ttctttttgc
    13381 ctatggggtc gcctggctcc ttgctgtgag tcatcgcaaa ggcgtgcttc ccggcgttct
    13441 gatggcggtg ctgctttgcg gagccgtggt cgcggccagc tggtacggga tcggaagact
    13501 gccccatgcg ctgctggtga ttgaggcggg ttacatcgtt cttgctgtgt tgatcgtagg
    13561 cacgattgtg ggtggcctgt ttgacaggct cgccctgccg cacaaagtcg taccctgaca
    13621 catctccctg ctcaacacga agctcaaccc cgtatcgaag gacctgctga ggagacgaac
    13681 tacggagacg ggttcgcggc gcgcaactcc gccacaatct cggcggcggc tttacgggct
    13741 tcgtcctcgc ggtcgggcgg gaacgagatc gcgcccacgc gggcgtggcc tcctccgccg
    13801 tagcgctcgc agatggtcgc aaggttgatc atcttctcag gcgcggtcct ggtccatgga
    13861 ttggacccca cagccacctt tgtgcgaaaa ctcgactttg acagccccac cgagtaagtg
    13921 gcttcaggat gaaggtagta cggaatgaac ttgttgtatc cctcgagggg atggtcggtg
    13981 atatcgaagt aaatcgtccc ctctttggac tcggcgcggg agcggatcag atcgatggcg
    14041 cgctggtggc gctcgaacag gggcggcagc agagaggcga cccagggctg ctcaaggacc
    14101 tgctgcagcg gcatgcgcgt gagcgtcggg atcaggcgcg ggatgaaggc gttgtcctgc
    14161 gtggactcaa tgatgagggt gagcttcatg gccggggcgg gcatttcgac ggcaagttgc
    14221 gggctctcgt agagggcgcc gtcgacgatg tcggcccagc gaacgagttc ggctaccggc
    14281 gtgggatcga agccgaactg cgtggacgcg atgtgggcga gaaagctggt gcaggatacg
    14341 tactcgggat catagaagcg atggccgcga taccgcccgt cggcgatgcc actgcggtaa
    14401 tcctggtggt cttccggcgt gagaaaagca gagagatggt gatcgaacca ccatgtgatt
    14461 ttgggcgacg gcgagtactt gaagtcgacg atggcattct cgtcgccgat gaagtccgcc
    14521 tcgttgaaga gggcgccggc gcgatgcacg aggccgcaga actcgtaggt tgcgtccggg
    14581 tggatgcgtt cctgatggaa acggctgaac agcgaggccg agcaggcccc gtcgaaacac
    14641 ttatcgtgat agaagacccg gaccttcaaa acgcgccgcc cccgcagaac cgccggcaaa
    14701 gggcgccggt cgggaatctc ttagattacc gggaaggcgg gaagtgtcaa gggtaacgag
    14761 cggaagcggc aactccgcgc gtccgctttt tttctattcg acagtgtttg gcaccctccg
    14821 ggctcatcga agtagccatg gagtgggtgg ctcagctcgt ccagtatttt ctggcgaaat
    14881 ggggatatct ggcgctggca actgcgctct ttggagagag cgcgggcctg cccctgcccg
    14941 gagagacggt tttgctgttt tccagcttcg tagcgcataa gaccggcgag ctgcgcatcg
    15001 ggctggtcat tctgattgga accactgcgg cgatttgcgg agacaatctc ggatatctgg
    15061 caggaagaca cttcggtccg cgcctgctga agtggctgaa gcgcaccttc aacatggagg
    15121 aagacgtcgc agcagccagt gctttgatcc gccgccacgg aggcgcaacc gtcttctggg
    15181 cgcgctacgt gattggcctg cgcgcgatcg ccgggccggt agccggcgcc ctgaacatgg
    15241 aatggaaacg atttctgctc tacaacgcgc tgggcggatt ctgctgggtc agctcgattt
    15301 ctctcaccgg ctatttcttt gcagcgcgcg tgcagtcgct gaccgggttc atcgaaaagg
    15361 tctcgtgggc aatcgccgga accgtgtttg ccgtgggcta tttgctgtgg cggcgcatga
    15421 agaagcagca aaaactgcgc acgcgacaac ggcaggccgc gtaacccgcc cttcgctatc
    15481 tggccgacag cttttccttg ttgggactcc agttgatgac atggtagacg gccatcgtcg
    15541 tcccgacgtt gcgaaagccg tgcaggctgt tcgaggcatt aaagacgacg gagccgggac
    15601 caaccttgac ccacttgcca tcggaaagcg tctcgcagtc gccttcgcgg atgatgatca
    15661 gctcttcgtt gacgtggcgg tgcggtggat gcgaggccgt tccagggttc agcttgctga
    15721 cgtgcatctc cagctgatcg acggtagccg tcggctgctt gcacagtgat gcgacttcac
    15781 ctgttgctgt cttgtggaat tgcagctggt cccactcaaa aacgtgaggg ccgagaattt
    15841 ccttgttctc cggcgcgggc gcgggctttg catcctgaac ggcttccgcc attgccgtga
    15901 gcacggcggg catcgtcatc agtcctgcga ctacattgcg gcgtgagatc atcgtcggtt
    15961 tctcctcctg gtgtggtgtt tctgaagttc ctttaccttg tcatcccgac cggaccctga
    16021 gcgtagcgaa ggggaagcgg agggacctgc ttctcttccg cataaagaaa tttcggaaac
    16081 accacactag ctgagccggt agggtgcacg ctgcggccgg gccagcatgc tgttggcttc
    16141 gtcgtcgttg tggaaccgct cgttcaccgg atcccagtag agtttgcgcc tgagcaccat
    16201 ggccatgtcg tgaatcaggc aggtggagca ggcgcgatgg cccatctccg cgggcgaaat
    16261 cggctgctgc cgcgtaacga tgcagtccag ccagttgccg tgctggtctt tgctctcgta
    16321 gagatggatc tcgtcgggac cgatggtcga cttgatgatc gccggatcgc tcgacgcaag
    16381 cgctgtgttg tcgttgagct tggcgaccgg atcgcttttg gtcacctgct cgttgccgcg
    16441 gctgacgaag atccaccctt tgctgccgta gaacttgatg ccgttgggat agtcgccgct
    16501 gatgctcatg tgaacgccgt tggcgtaatt ggcttccgtt ttgaacgggc cgtgcacgtc
    16561 ccaaaggccg tgatcgggga attgggcctg tccccagatc tcaatcgggc cggtgtactc
    16621 ggtgttcatc ccccagtgag cactgtcaat gtgatgagcg ccccaccccg tgatcatgcc
    16681 ggcgccgaac tggcggcagc gcagccatcc gggacggtcg tagcccttcg gcggatgcac
    16741 gcggtcgacg gtgtagtaaa cgtagggcgt ttcgcccagc cacatctcgt agttgaagcc
    16801 gtcgggcacc ggcatcggcg ttttgtcgcc gccggcggga tcaccaggca ggccgatctc
    16861 gacccgctgc aggtcgccga tgcgtccgtt gcggaccagc tcggcggcgt agcgaaactg
    16921 aatcgtggag cgctgctggc tgccgatctg
//
LOCUS       DASZYN010000683         8096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1132744, whole genome shotgun sequence.
ACCESSION   DASZYN010000683 DASZYN010000000
VERSION     DASZYN010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8096
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132744"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2538)
                     /locus_tag="VHE33_17740"
     CDS             complement(<1..2538)
                     /locus_tag="VHE33_17740"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HVT99350.1"
                     /translation="MSDSRSSPGIRESLSPLKQAFLALEEAEQRIRQLEQNVSEPIAI
                     VGMGCRFPGAENGPEDFWRLLHTRQCAVGDAVEARLGLSFERDHLPPQARWAALLDRV
                     DLFDPRHFGIAPREAGAMDPQQRLLLEVTWEALENAGIDPNSLYQTQTGVYVGIASHD
                     YAQLAVRHQRPDQIDQYFAAGSGASVASGRISYVLGLNGPALSVDTACSSSLVAVHLA
                     CEALQRGACTTALAAGVNLILSPEACAAFAQAGMLSPRGICAAFDENADGFVRGEGCG
                     VVVLKRLRDAEAAGDRVLAVILGSAVNQDGASSGLTVPNGLAQQALMREAHRRAGIEA
                     HQVGYIETHGTGTKLGDPIEAEALGSVFSGRASKLLMGSVKANIGHLESAAGVAGLIK
                     LVLSLEHGEVPGQVHWRQPSSHVRWQELPLEVDPENHTWEPIEGRRIGGVSSFGFSGT
                     NAHVVVEGRKPTAREESAETGEDVLVISARTQPALRELAERYVKFLEGDTDYSWAEIC
                     HTAGAGRAAMSDRLALVACSRGEAAEKLRAWLRGETSQGISSGHVGAGQRGVLQPDVH
                     ATPEQIASCFVQGAKVNWERRRGERRRRRAPLPRYPFQRERYWIEERKKTLEIGESTG
                     RGMLGSRLCVAGVRAQYETQLDADSWVGEHLVEGRVILPATGHIELMLEAGAETLGGR
                     CVIEDLVLQATLDVEGERRVHTVVDKEVAGRSRVRIYAQRGEGDWDQVSEGWLRRAEK
                     AEAERRDLDPLRDRLQERDAGDAFYSSLRCRGLEFGDRFRGLRRLWSGEGEALGEIAT
                     ATDTNGNWELAPWALDACIQVAAAIGEPEALYLPMSMERIEVFRSMPE"
     gene            complement(2535..5675)
                     /locus_tag="VHE33_17745"
     CDS             complement(2535..5675)
                     /locus_tag="VHE33_17745"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HVT99351.1"
                     /translation="MATADKSAAATPSPGLSPVKQALVEIRRLRAELDDLRRGQREPI
                     AVIGMSLRLPGGVRSPERFWQALAAGEDLIGTIPPERWHADAWRGAGPDEAGTIYDVH
                     GGFLDDIDSFDADFFGIHARQAASMDPQHRILLELSWEALERSGIDPRGLADSSTGVW
                     LGLSNSDYGRKLTSDPLRIDGYTGIGAAASMAAGHIAYLLGLHGPAEVIDTACSSSLV
                     AVHRAVQSLRSGETSLAIVGGANLMLSPEIHVSFSRTGMLSRSGRCHTFDAKADGYVR
                     SEGCAVVVLRRMSDAMRLGDRVLAVIRGTAVNQDGRSAGITAPNGPAQEAVMRAALRD
                     ANLGADTIGYVETHGTGTPLGDPMEWRATGEVYGEGRSTDAPLYVGSAKTNLGHTEAL
                     AGLVGLMKAILMMDPAHGIPPHLHFEDPNPKIDWERWPIKVPIGQTPWPGESSVRRIA
                     VSSFGFSGTNCHLILESPEPNNIATRAELPLTTFQHTRFWFGDSPLAEWQQALDRGWR
                     AALSAAEAHSHLGPLGWKPESFPERWAVLEKLTLAHASSTLAALSAFPHGTARTLDEL
                     IERCAIRPIYRRLVQRWLRGLVRAGQFVESDGSYAPATGGRSDDLESCWREAEHRLAD
                     EPGMLAYFRRCGMLLPAVLTGKVDPLETLFPDGSFALAEAIYEEGPEARYSNRVVAEA
                     VRAFVEQRKENRTMRLLELGGGTGGTTAAVLPQLRNVPVQYWFTDVSELFLNRARRKF
                     ASFDFLRYALFDLDQNSKDQPLRNGSFDLILAANVVHASRNIPAALERIHQLLAPGGA
                     LVLLETTTHQACFDMSIGLIEGWQHFEDSERGDHPLLNAEQWCEVLRRSGFEETTALP
                     EAGSLASALGQHVLLARRGVREVLISGSGATTGQSEPAVHTPIRPAMPPVSSLSVDLA
                     ALSESEREQTVAQAVRQTICRVFGLQMAADELGDRDRLSDLGMDSLIALELRGELAKA
                     LGLEGRIPSTIAFDTGTVGELTRSLVSLLHPSSGRPGEFETAHADAPATVTAEDLRGM
                     TEEQVEQLLMERMRKR"
     gene            complement(5659..>8096)
                     /locus_tag="VHE33_17750"
     CDS             complement(5659..>8096)
                     /locus_tag="VHE33_17750"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT99352.1"
                     /translation="LLRENIQPAMGVWLMTAGGVGPEAASPAQAAVWAIARTASVEFP
                     DLSVRCVDIGDGEISGDLLSSLARSSARTVLVRGGRFFQSELVRQTKTTAESGNTSLR
                     ASPSGLIDLLEEVPVPRCEPRPDEVEIRIEAHGINFRDVLTALGMLSVTNPVLGGECA
                     GTIARAGAQSGLNPGDRVFAFAPHSLQAFVTVPVVNVARIPEGFTAEQASALPIVYLT
                     AIYGLDRLASLQRGESVLIHAAAGGLGLAALHLARARGAEVCATAGSDEKREYLRKLG
                     VRHVFSSRTIDFAEGVLQATGGRGVDVVLNSLTGDLAEKNLSVLAPRGRLLEVGKRDT
                     LSLQEVRQRRPDVGYFIYDLGEEAERDATLIPALMAVLLRMMQEGSLPPLPVTSFSDV
                     REAMRYMAQARHIGKIVVTRSFDEHSKVPVSPHATYLITGGCGGLGLIFAESLVSRGA
                     RSLVLMGRSAPSPSALAQIELMRSRGAQVTVFRGDVADRTTIEAALNAIPAGRPLKGV
                     LHVAGVLDDRSLLLHNQESLLAAMRPKWLGAWNLHQATETLPLDFFILFSSAVVSLGS
                     PGQANYAAANATLDALAAWRKKRGLPALSVQWGPWAAGMAEHLKASLENLGLGRLAAA
                     EGVTALDRALNVDEPVVAILPVTSWHRLQQWQRKGERGSARHASSDSTAISAGQPARG
                     IVERLSDMAPAERHAFLAEHLRQQVIHILSLPSTTRIDEDEALHDLGLDSLMAVELRN
                     ALATSLGQPWSPTMVLDYPTLRSLTGYLLSELFLSAEQSQAAAQPIENLSEAEAEELL
                     LQELSGGKHGDGR"
BASE COUNT         1553 a         2634 c         2407 g         1502 t
ORIGIN      
        1 ctccggcatg gaccggaaga cttcgattcg ttccatgctc atcggcaaat agagagcttc
       61 cggctcaccg attgccgcag cgacctggat gcacgcgtcc aaagcccatg gagcgagctc
      121 ccaatttcca tttgtatctg tcgctgtggc aatttctccc agcgcttcgc cttctccgct
      181 ccacagccga cgtaagccgc gaaaccggtc gccgaactcc aggccccggc atctcaggga
      241 ggaataaaat gcgtcgccag cgtctctctc ctgcagccgg tctcgcagtg gatcgaggtc
      301 tcgtcgctcg gcttcggctt tttccgctcg gcgcagccat ccttcggaga cctggtccca
      361 atcaccctca cctcgctggg cgtagatacg cactcggcta cggcccgcca cctctttgtc
      421 tacgacggtg tgcacacgcc gctcgccttc cacgtcgagc gttgcctgca gcaccaggtc
      481 ctcgattacg caccggccgc ccagggtctc tgctcccgct tccagcatga gctcgatgtg
      541 tccggtagcc ggtagaatca cacgaccctc gacgaggtgc tcgcccaccc agctgtccgc
      601 gtcgagctgc gtctcatact gcgcacgcac gccggcgaca cacaaccgac ttcccagcat
      661 tccccgcccg gtggactcgc cgatttccag cgtttttttc cgctcttcga tccagtaacg
      721 ttcgcgctga aatggatatc gaggcagggg agcccgccgg cgccgacgct cgccccggcg
      781 ccgctcccag ttcaccttcg cgccctgcac aaagcaggaa gcgatctgct ccggcgttgc
      841 gtggacgtct ggctgaagta caccgcgctg gcctgctccc acgtgaccgc tgctgatccc
      901 ttgcgaggtt tcgccgcgca accatgcccg caacttctcc gctgcttcgc cgcgactgca
      961 agccaccagc gccagccggt cgctcatcgc cgcacgtccc gccccggctg tatgacagat
     1021 ctccgcccag ctatagtctg tgtcaccttc cagaaacttc acgtagcgct cggcaagctc
     1081 tcgtaacgcc ggttgcgttc gggccgagat cacgagcacg tcctcgcctg tttccgccga
     1141 ttcttccctt gctgtgggtt ttctgccttc gaccaccacg tgcgcgttgg ttccgctgaa
     1201 cccgaatgaa ctcacgcctc cgatgcggcg tccctcgatc ggctcccagg tatgattctc
     1261 cggatccact tcgagcggca attcctgcca gcgcacgtga ctgctcggtt gccgccagtg
     1321 cacttgtccc ggaacttcgc cgtgctcgag gctcagcacc agtttgatca accccgccac
     1381 tcctgccgcc gattccagat ggccgatgtt ggccttcacc gatcccatca gcaacttgct
     1441 ggcgcgaccg gagaacacag agcccaacgc ctcggcctcg atcggatcac ccagcttcgt
     1501 cccggttccg tgagtttcga tatatcccac ctgatgcgct tcaattccgg cgcggcgatg
     1561 cgcttctcgc atcagtgcct gctgcgccag tccatttggg accgtcagcc cgctggaggc
     1621 gccgtcctga ttcacggccg aacccagaat cacggccagc acccggtctc cggctgcttc
     1681 cgcatcgcgc agacgcttca gcaccacgac tccgcagcct tcgccgcgca caaatccatc
     1741 tgcgttttcg tcgaatgccg cacagatgcc gcgcggcgac agcatgccgg cctgggcaaa
     1801 cgcggcgcag gcttcgggac tgagaatcag attgacaccg gcggccaggg ctgtggtgca
     1861 cgcacctcgc tgcagcgcct cgcaggccag atggacagca acgagagaag aagagcaggc
     1921 cgtgtccacc gagagcgccg gaccgttcaa cccaagcaca taggagattc gcccggatgc
     1981 aacactggct ccgctcccag cggcaaaata ctgatcaatc tggtctgggc gctggtggcg
     2041 cacggccagc tgcgcgtagt catggctcgc gatgcccacg taaacgccgg tctgggtctg
     2101 atagagagag ttgggatcga tgcccgcgtt ctcgagagct tcccaggtca cttcgagcag
     2161 aagacgctgc tgcggatcca tcgctccggc ttcgcgcggc gcgattccaa agtgacgcgg
     2221 atcgaaaagg tcgacgcgat ccagcaacgc agcccagcgc gcctgtggcg gtaaatggtc
     2281 gcgttcaaag gaaaggccaa gacgggcttc gacggcatcg ccgaccgcac attggcgagt
     2341 gtgcagcagg cgccagaaat cttcgggccc gttttcggca ccgggaaaac ggcatcccat
     2401 gccgacaatg gcgatcggct cggatacgtt ctgctcgagc tggcggatgc gctgctcagc
     2461 ctcctccagg gccaggaacg cctgcttgag gggagacagc gattctcgaa ttccgggtga
     2521 tgatcttgaa tcgctcatcg cttcctcatc cgctccatca gcaactgctc gacctgctcc
     2581 tcggtcattc cccggagatc ttccgctgtc accgtagcgg gagcatccgc atgcgcagtt
     2641 tcgaattcac caggcctgcc ggaggaggga tgcagcagac tcacgaggga ccgcgtcagt
     2701 tcgccgaccg tgccggtgtc aaaggcaatg gtggatggga ttcgtccttc gagtccgaga
     2761 gccttggcca attcgccgcg cagctcgagc gcgatcagag aatccatgcc aaggtcactg
     2821 agccggtctc gatctcccag ttcgtctgcg gccatctgca aaccgaagac gcgacagatg
     2881 gtctgccgca cggcttgcgc caccgtttgc tcacgttcgc tctcgctcag tgcagcaaga
     2941 tcaacgctca aagaagaaac aggaggcatc gctggtctga tcggcgtatg aacggccggc
     3001 tcgctctgtc cggtcgtcgc tccggagccg ctgattagca cctctcgcac cccccgccgc
     3061 gccagcagca catgctgacc gagagcagag gcgagcgatc cggcctccgg cagagctgtc
     3121 gtttcctcga aaccgctacg gcggagcact tcacaccact gctcggcatt gagtagtggg
     3181 tgatcgccac gttcgctgtc ctcaaaatgc tgccagcctt cgatcaaccc gatcgacatg
     3241 tcgaagcagg cctggtgtgt ggtggtctcc agaagcacga gagcgccgcc aggggccagc
     3301 aactgatgga tgcgctccag cgccgctgga atattgcggc tggcgtggac cacgttcgca
     3361 gcgaggatca agtcgaagga tccgttccgg agaggctgat ccttcgaatt ctggtcaaga
     3421 tcgaaaagcg cgtatcgcag gaagtcgaag gacgcaaatt tccttcgtgc gcgattcaga
     3481 aagagctccg atacgtcagt aaaccagtac tggacgggga cgttgcgcaa ctgcggcagg
     3541 accgcggccg ttgtgccgcc ggttcccccg ccgagttcaa ggaggcgcat cgttcggttt
     3601 tctttccgtt gttcgacgaa cgctcgaacc gcttcggcaa cgactcggtt gctgtaccga
     3661 gcctccggcc cctcttcata gatcgcttcc gccagagcga atgagccgtc cgggaaaagt
     3721 gtttcgagtg gatcaacttt gcccgtgagc acggcaggca ggagcatgcc gcagcgacgg
     3781 aagtacgcga gcatgccggg ctcgtctgct aagcggtgtt ctgcttcgcg ccagcaggat
     3841 tcgagatcgt ccgagcgccc accggtggca ggggcgtagg atccatcgga ctcgacgaac
     3901 tgtccggcgc gaaccagccc ccgcagccag cgctgcacga ggcgccggta aatcggccga
     3961 attgcacagc gctcgatcag ctcatcgagg gtccgggcag ttccgtgagg gaacgcggac
     4021 aacgcggcaa gggtcgaaga agcatgcgcc agcgtgagtt tttccaatac ggcccagcgc
     4081 tccggaaaac tctccggctt ccagcccagg ggtcccagat gactgtgagc ctctgcggcc
     4141 gatagcgctg cacgccatcc gcgatcgagt gcctgttgcc actccgccaa aggcgagtcg
     4201 ccaaaccaga aacgcgtgtg ctgaaaagtc gtcagcggca gttcagcgcg cgtagctatg
     4261 ttgttaggct ccggagattc aagaatcagg tggcaattcg tgccactgaa tccgaaggag
     4321 ctgacggcga tccggcgaac actggattct cccggccatg gcgtttgacc gatgggcacc
     4381 tttatcggcc accgttccca atcgatcttc gggttcggat cttcgaagtg aagatgcggt
     4441 gggatgccgt gagcgggatc catcatcagg atcgctttca tcagaccgac aagaccggcg
     4501 agagcttccg tatggccgag attggttttc gctgagccaa cgtacagagg tgcatccgta
     4561 ctacggcctt cgccgtacac ctcgccggtt gcccgccact ccatgggatc tccaagcggc
     4621 gtgcccgtgc cgtgcgtctc gacatagccg attgtgtccg cgcccaggtt cgcatcgcgg
     4681 agagcagcac gcatcacggc ctcctgagcc ggaccattag gagccgtgat tccagcgctg
     4741 cggccatcct ggttaacagc ggtaccgcga atgaccgcaa gaacgcggtc gcccagacgc
     4801 atggcatcgg acattcgtcg cagcacaaca acagcgcagc cttccgagcg aacatagccg
     4861 tcggccttcg catcgaaggt atggcagcgc ccgctgcgcg acaacatgcc ggtgcgtgag
     4921 aagctcacgt ggatctccgg cgagagcatc agattggcgc cgccaacgat ggcgaggctg
     4981 gtctcaccgg atcggaggct ctgcaccgcg cggtggactg ccaccagtga agaggagcac
     5041 gcagtgtcaa tcacttcggc aggcccatgc agtccgagca gataggcgat gtgtccggcc
     5101 gccatgctgg ccgccgcgcc aataccggta tatccgtcaa tacggagtgg gtcgctggta
     5161 agcttgcgcc cgtaatcgct gttgctcagg cccagccaga caccggtgga cgaatcggcg
     5221 aggcctcgcg gatcgatacc ggatcgctcc agagcttccc agctgagttc cagcagaatt
     5281 cggtgctgcg gatccatgct tgcggcttgg cgcgcatgga tgccgaagaa atccgcatcg
     5341 aagctgtcga tatcgtcgag gaaacccccg tgaacgtcat agatcgtacc ggcttcgtcg
     5401 gggccggcgc cacgccaggc gtcggcatgc cagcgctccg gaggtatggt gccgatgaga
     5461 tcttcgcccg cagccagtgc ctgccagaag cgttccggcg agcggactcc accggggagt
     5521 cggagcgaca tgccgatgac agcgatgggc tctcgttgtc ctcgccggag gtcgtcgagt
     5581 tcagcgcgta gacggcgaat ttccaccagc gcctgcttca ccggggacag gccgggcgag
     5641 ggagttgccg ctgcggactt atcggccgtc gccatgcttc cctccactca actcctgcag
     5701 cagaagttct tcggcctctg cttcgctgag attttctatc ggttgcgccg ctgcctggct
     5761 ttgctcagcc gataagaaca attcggacag taaatatccc gtcaggctcc gcagagtcgg
     5821 gtaatccagt accatggtcg gcgaccacgg ctgtcccagc gaggtggcga gcgcattgcg
     5881 gagttcgact gccatcagtg agtcgaggcc gagatcgtga agggcctcat cctcgtcgat
     5941 acgcgtcgta gacggcaggg acaggatgtg aatcacctgc tggcgcaggt gctcggcgag
     6001 aaaagcgtgt ctctcggctg gcgccatgtc actcagccgc tcgacgattc cgcgcgcagg
     6061 ctgcccggcc gagattgcag tggaatcgga tgacgcgtgc cttgccgatc cgcgctcacc
     6121 tttcctttgc cattgctgga gtcggtgcca ggacgtaacg ggcagaatgg cgacaacagg
     6181 ttcgtcaacg ttcagcgcgc gatccagcgc ggtaactccc tcggcggccg cgagccgacc
     6241 aaggcccagg ttctccaggc tggccttcag atgttcggcc attccggcgg cccacgggcc
     6301 ccactgcaca ctgagcgctg gaagaccacg ttttttgcgc caggcagcaa gcgcatcgag
     6361 ggttgcgttc gcggcggcat agttcgcctg tcccggagat ccgagcgata cgacagccga
     6421 ggagaagagg ataaagaagt cgagcggtaa ggtttcggtt gcctggtgca ggttccaggc
     6481 accgagccat ttgggccgca tcgcagcgag cagagattcc tgattgtgca acagcaggct
     6541 gcgatcatcg agcacgccag ccacgtggag gacacccttg aggggccgac ctgcgggaat
     6601 cgcgttgagg gcggcttcga ttgtggttcg atcagcaaca tcaccgcgaa agacggtgac
     6661 ctgcgccccc cgcgagcgca tcagttcaat ctgcgccagc gcagagggag agggggcgct
     6721 gcgacccatc aacaccaggc tgcgagcacc acgactgacg agtgattccg caaagatgag
     6781 tccaaggcca ccgcaaccac cggtgatcag gtaggtcgca tgcggagaga cgggaacctt
     6841 tgagtgctcg tcgaaggacc gcgtgacgac aatttttccg atgtgccgcg cctgcgccat
     6901 gtaccgcatc gcctcgcgca catccgaaaa gcttgtcaca ggcagaggag gcagagatcc
     6961 ctcctgcatc atgcgaagca ggactgccat caacgcgggt atcagcgtgg catctcgctc
     7021 ggcttcctct ccaagatcgt agatgaaata gccgacatca ggccgacgct gccggacttc
     7081 ctgaagactc agcgtgtctc gcttgccgac ctcgagcaga cggccccggg gggccaggac
     7141 gctgaggttc ttctccgcca ggtcgccggt cagcgaattg aggaccacat ccacacctcg
     7201 gcctccggtc gcctgcagca ctccttcggc aaagtcgatc gttctggagg aaaagacgtg
     7261 ccgcacgccg agcttacgca aatactcccg cttttcgtca ctgcctgctg tcgcgcagac
     7321 ttcggcgccg cgcgccctgg ccagatgcag tgcggctaat ccgagtccgc ccgcggctgc
     7381 atgaatgaga acactctccc ctcgctgcag cgaggccagt cgatccagac catagatcgc
     7441 cgtcagatag acaatgggca aagcggaagc ctgctccgct gtgaagcctt ccggaatacg
     7501 agccacattt actacaggca cggttacaaa tgcctgcaga ctatgtgggg caaaggcaaa
     7561 aacccgatcg ccagggttta aaccactttg cgctcccgcg cgcgcaatgg ttccagcgca
     7621 ttctcctccc agcaccgggt ttgttacgga caacatgccc aatgccgtca gcacgtcgcg
     7681 gaaattgatg ccgtgagctt cgattcgaat ttcgacctca tcgggtcgcg gctcacacct
     7741 cggcaccgga acctcttcga gaagatcgat cagccctgag ggcgatgcgc gaagggatgt
     7801 attgccggat tccgcggtgg tctttgtctg tcgcacgagc tctgactgga agaaccgtcc
     7861 tccgcgaacc agcactgtcc gggcgctgct ccgagcgaga gaactgagca ggtctccgga
     7921 gatttcaccg tcgccgatgt ccacacatcg aaccgaaaga tccgggaact ccacggaagc
     7981 ggtgcgtgcg atggcccaaa cagcagcctg ggctggggaa gctgcttccg gtccgacacc
     8041 ccccgcggtc atcaaccaca cccccatcgc gggctggatg ttttctcgca acagaa
//
LOCUS       DASZYN010000684         5415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_738299, whole genome shotgun sequence.
ACCESSION   DASZYN010000684 DASZYN010000000
VERSION     DASZYN010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738299"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VHE33_17755"
     CDS             complement(<1..244)
                     /locus_tag="VHE33_17755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99353.1"
                     /translation="MQKNQSGLSRRGLLKGIGGLGIAAASPKVFAGSAIYPSAPPVDP
                     PPSPITPQLTSFALGDVDLLDGPFLQARLRCQNYLLS"
     gene            complement(383..1309)
                     /locus_tag="VHE33_17760"
     CDS             complement(383..1309)
                     /locus_tag="VHE33_17760"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HVT99354.1"
                     /translation="MSLLRSTFISLSRNSTLRHFSEQSSFGRRMSSRFVAGFRIEDAL
                     AAAASLNSQGLSVTLDSLGENVTTPDQARQSAQVYHWLLDAIAQGKLDANVSLKLTQM
                     GMDLGPETVTETVSSLVDHAVQADSFVRVDMEGTAYTQATIDLVRRLHAQPENGGHVG
                     IVIQAYLRRSADDVAALLRDRIRIRLCKGAYKEPPDLAFPEKKDVDANFVALTKVLLK
                     SGVFHGIATHDPAMIAATKDFARAERIDPKTFEFQMLYGVRRDLQRSLVQEGYRVRVY
                     VPFGDEWYPYFMRRLAERPANVLFLARNFFRK"
     gene            1696..1917
                     /locus_tag="VHE33_17765"
     CDS             1696..1917
                     /locus_tag="VHE33_17765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99355.1"
                     /translation="MHYLREGRIFVFDVTSGAADKNGKRSRRMEHYWLCGECSQTLAM
                     EQSADGVKVVPRARGFVQEASLARSAMAS"
     gene            complement(2044..4818)
                     /locus_tag="VHE33_17770"
     CDS             complement(2044..4818)
                     /locus_tag="VHE33_17770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HVT99356.1"
                     /translation="MGWTHRLRSLFRRQAHARDLEEELNFHLAMREQRNREHGLSPQE
                     ARRQARLRFGNPTLLRERITELDLILLPQTVWQDLRFGARILLRQWGLTLTAIFALAL
                     GIGVSTAAFSAYKAFFARPLDARHPQTMTNLAVILHTGVTTPVFSYPDYVEYRDRLHA
                     FSAIIATSLPQFLPVRTPGGTLVQHDEGAGSLIGRLFPLAGSTNNERALSMFVSENFF
                     SVLGVQAVRGQVFRQQDTRQLATSPAVLISGNYWQRKFDSDPSIVGTRVVLNGASFTI
                     MGVTPRNFVGTFVAAPDFWIPLSLEPLVHPEQNWLTDREKFCCHLRARLAPGVSLHQA
                     QSEMSLLADRLRALHQAQSDWAQPLHAVVWPGSPFPIPPSQNRNIVIALSLVALAVAL
                     VLIVACANVASLQLARAHARHNELSMRLSLGASRLRLMRQLLTESALLAVIAGALAFL
                     FSWAFLQWAVTAAANAFPDEYGTFVFHVSPDLSVFTFVLGISLAAGILFGFAPALDSS
                     RSAIAASLRANNFVSLGRHRLRSLLIASQVALSAVLLIAGSLLIHSAIRALNMDTGYD
                     DAHVVDLNLQFPENAGYSADRKTALVHQLTARLAALSGVADVTSARAPDDPDFRDAAV
                     SINSDAPSRTNTKAFLFYTYIEPNYFRTLGIPLLFGHPFADTAEDSAILSESAAKALW
                     PGENPIGKTLRLSTERQFHGPAEPLPDGPSWRVIGVARDTRGVLLDGSDSAQVYLPMP
                     AAAAAQYPILVHTRISPATLIVQIGPLLASVDPALMMRAITLEDMLRSSAPFIASTVS
                     AAIASVTGLLGLLLVTIGIYGTVSYVVVQRTHEVGIRMALGARKANVIALILRDSSRP
                     VLFGLGIGLILAAGAAWLLRHVLYGIDLIDPLSFGGVSVLLIAVALLAAFLPSRRAAR
                     VDPMVALRCE"
     gene            complement(4830..5174)
                     /locus_tag="VHE33_17775"
     CDS             complement(4830..5174)
                     /locus_tag="VHE33_17775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVT99357.1"
                     /translation="MKQKKTDTLQGTLDLLVLTTLEQGPLHGWGITLHIERVSDAILR
                     VEEGSLYPALHRMEQEGWITASWGMSENNRRARYYQLTRLGRKQLHAERENWARITGA
                     VAQILAFGVSGT"
BASE COUNT         1083 a         1310 c         2107 g          915 t
ORIGIN      
        1 gactcagcag atagttctgg cagcggaggc gagcctggag aaaggggccg tcgagcagat
       61 ccacatctcc gagagcgaac gaggtgagct gtggcgtgat gggcgacggc ggcggatcga
      121 ccggcggtgc ggaggggtag atcgcggagc ctgcaaaaac cttcgggctt gcggcggcga
      181 ttcccaaacc cccaattccc ttgagaaggc cacggcgaga aagcccgctt tgattcttct
      241 gcattgctga aagcgctccg tgactggatg atacgtcacg actgaaagcg aatcaccgct
      301 ggcgacggga gggctgggaa cgcagaccgg cctggcacaa agcaacggcc tggccatcga
      361 aagggtcttc gcgacgccgc gtctatttcc tgaaaaagtt gcgggcgagg aagaggacgt
      421 tggcggggcg ttcggctaag cggcgcatga agtaggggta ccactcgtcg ccgaagggga
      481 cgtagacgcg gacgcggtag ccttcctgga cgagggaacg ctggaggtcg cggcggactc
      541 cgtagagcat ctgaaactcg aaggttttgg ggtcgatgcg ctcggcgcgg gcgaagtcct
      601 ttgtggcggc gatcatggcg gggtcgtggg tggcgatgcc gtggaagacg ccgcttttca
      661 acaggacttt ggtgagagcg acgaagttgg cgtcgacgtc tttcttctca ggaaacgcga
      721 ggtcgggcgg ctccttgtaa gcgcctttgc agaggcggat gcgaatgcgg tcgcggagca
      781 gagcggcaac gtcgtcggcg ctgcggcgga ggtaagcctg gatcacgatg ccgacgtggc
      841 cgccgttttc gggttgggca tggagccggc ggacgaggtc gatcgtggcc tgggtgtagg
      901 ccgtgccttc catatcgaca cggacgaagg agtcggcctg gacggcgtgg tcgaccaggg
      961 aggagacggt ctcggtgacg gtttcgggcc ccaggtccat gcccatctgg gtgagcttga
     1021 ggctgacgtt ggcgtcgagc ttgccctggg cgatggcgtc gaggagccaa tggtagacct
     1081 gagcggattg ccgcgcctgg tccggggtgg tgacgttctc gccgaggctg tcgagggtga
     1141 cggagaggcc ctgggaattg aggctggcag ccgcggccag ggcgtcctca atgcggaagc
     1201 cggcgacaaa gcgggacgac atgcgccggc cgaagctgga ttgctcggaa aaatggcgga
     1261 gggtggagtt acgagagagg ctgatgaagg tggaacgcag cagggacatt tcgttcgaga
     1321 ggcaaacgat cattcttgca caagcgtcac gccttgacgt cgattttcac cgaattttgc
     1381 ggtactttcg ggtgcagctg tgccagaata gaaaaagtta accgtcgaat gcagggccat
     1441 cgtgacctcg gtgctgttcc ggctaacctg gcagaaaaca gaacgcgggt ttttctgcat
     1501 cctattgcaa gtaaatcgag tatgagtcgt gcgcgggata caagcgcgaa tcccgcagag
     1561 tctttctttc aacagcagca gcgctcagga tcgctcgcaa ggcagcgatt ctggaggttt
     1621 cgccccggaa gggcgagcgc agagtggaga tgggtcatgg tgaatcattg cgcaaataca
     1681 aggtgctcga agcctttgca ttatttgcga gagggacgga tcttcgtttt cgacgtgacg
     1741 tcgggagcgg cggacaagaa cggaaaacgc tcgcggagga tggaacatta ctggctatgc
     1801 ggggagtgtt cgcagaccct ggcgatggaa cagtctgcgg atggagtgaa ggtggtgccg
     1861 cgggcccggg gctttgtgca ggaagcgtcg ctggcgcgat cggcgatggc ttcgtaagag
     1921 tcctgagaaa aaccgagcag aaaaaggcgc cctttgcggg cgccttttgg tttttgcggg
     1981 atgatcgatg ggctgggtcc catgggaatc ctgcactctg ctgttgaaac gtgtggtttt
     2041 ctgtcactcg cagcgcaggg cgaccatggg atcgacacgg gcggcgcggc gcgaggggag
     2101 gaaggcagcg agaagggcca cggcaatcag caggacagag acgccgccga aggataacgg
     2161 atcgatgagg tcgatgccgt agaggacgtg gcggaggagc caggcggccc cggctgcgag
     2221 gatgaggccg atgccgagtc cgaagaggac ggggcgggag gagtcgcgca ggatgagcgc
     2281 gatgacattg gcttttcggg cgccgagcgc catgcggatg ccgacttcgt gggtccgctg
     2341 gacgacgaca tagctgacgg tgccgtagat gccgatggtg accagcagaa gtcccagcag
     2401 tccggtgacg gaggcgatgg cggcggagac agtggaagcg ataaacggcg cggaactccg
     2461 cagcatgtcc tcgagggtga tggcgcgcat catgagggcg ggatcgacgg aagcgagcag
     2521 ggggccgatc tggacaatca gcgttgcggg gctgatgcgg gtgtggacga ggattggata
     2581 ctgggccgct gcggcggccg gcatgggcag ataaacctgg gcagaatcgc tgccgtcgag
     2641 gagaacaccg cgcgtgtcgc gggcgacgcc gatgacgcgc caggagggcc catcgggaag
     2701 gggttcggca gggccgtgaa actgccgttc agtgctcagg cggagagtct tgccgatcgg
     2761 gttttcgccc ggccagaggg ctttggcggc ggattcgctg aggatggcgg aatcctctgc
     2821 ggtgtcggca aaggggtggc cgaagagaag aggaatgccg agggtccgga agtaattcgg
     2881 ctcaatgtag gtgtaaaaga gaaacgcttt ggtgttggtg cgagagggcg catcgctgtt
     2941 gatggagacg gcggcgtcgc ggaagtcagg atcgtcggga gcgcgggcgc tggtgacgtc
     3001 ggcgacgccg gacagggcag cgagccgggc agtgagctgg tgcacgaggg ccgttttccg
     3061 atccgccgag tagccggcgt tttcagggaa ctgaagattg agatcgacga cgtgagcgtc
     3121 gtcgtagccg gtatccatgt tgagggcgcg gatcgcgctg tgaatgagca ggctgcctgc
     3181 gatgagaagc acggcggaca acgccacctg agaggcgatg aggagactgc gcaggcgatg
     3241 gcgtccgagg gagacgaaat tgttggctcg gagagatgcg gcgatggcgg agcgggagct
     3301 atcgagggcc ggagcgaagc cgaagaggat gcccgccgcg agagagatgc caaggacaaa
     3361 ggtgaagacc gacaggtcgg gcgagacgtg aaagacgaag gttccgtatt catcggggaa
     3421 ggcgttggcg gcggcggtga cggcccactg caggaaagcc cagctgaaga gaaaggcgag
     3481 ggcgccggcg atgacggcca gcagagcact ctctgtgagc aattgccgca tgaggcggag
     3541 gcggctggcg ccgagagaga ggcgcatgga gagttcgttg tggcgcgcgt gcgcgcgagc
     3601 caactgaaga ctggcgacat tggcgcaggc cacgatgagc acgagggcga cggcgagggc
     3661 cacgagggag agggcgatca cgatgttgcg attctggctg ggcggaatgg gaaacggtga
     3721 gccgggccag accacggcgt gcagaggttg tgcccagtcg gactgcgcct ggtggagggc
     3781 gcggaggcga tcggcgagga gtgacatctc ggactgtgcc tgatgcaggc tgacgccggg
     3841 ggcgaggcgg gcgcgcaggt ggcaacagaa cttctcgcga tcggtgagcc agttctgttc
     3901 aggatggacg aggggctcga ggctcagcgg gatccagaag tcgggagcgg caacaaaggt
     3961 tccgacgaag ttgcggggcg tgacgcccat gatggtgaac gacgcaccat tgagaacgac
     4021 cctggttccg acgatggagg gatcgctgtc gaattttctc tgccagtagt tgccgctgat
     4081 cagaacggcg ggagaggtgg cgagctggcg ggtatcctgc tggcgaaaca cctggccgcg
     4141 aacggcctgc acgccgagca cggagaagaa gttttcagag acgaacatgg agagagcgcg
     4201 ctcgttgttg gtggagccgg cgagcggaaa cagcctgccg atgagagatc ctgcgccttc
     4261 gtcatgctgc acgagtgttc cgcccggggt tcgaacgggg agaaactggg gcaggctggt
     4321 ggcgatgatg gcggagaagg cgtgcagacg atcgcgatac tcaacgtagt cggggtagct
     4381 gaagacgggt gtggtgacgc cggtgtggag aatgaccgcg agattggtca tggtctgggg
     4441 atggcgcgcg tcgagcgggc gtgcaaagaa agctttgtaa gcgctgaagg cggcggtgct
     4501 gacgccaatg ccgagagcaa gggcaaagat ggcggtgagg gtgaggcccc attggcgaag
     4561 gagaatgcgg gctccgaagc gcaggtcctg ccagacggtc tgcggcaaca ggatgagatc
     4621 gagctcggtg atgcgttcgc gcaggagtgt ggggttaccg aagcgcaggc gagcctggcg
     4681 gcgcgcttcc tgtggagaga gaccgtgttc gcgattgcgc tgctcgcgca tggcgaggtg
     4741 gaagttcagc tcttcttcca gatcccgcgc gtgagcctgg cggcggaaca gcgagcgtag
     4801 tctgtgagtc cagcccatgt gcacgccttt caggttccgg aaacgccgaa ggcgagaatc
     4861 tgcgcgacgg caccggtgat ccgcgcccag ttttcacgct cggcgtggag ttgtttgcga
     4921 ccgagacggg tgagctggta gtagcgagcg cggcggttgt tttcgctcat gccccaggag
     4981 gcggtgatcc agccctcctg ctccatgcgg tggagggcgg ggtagaggga gccttcttcg
     5041 acgcggagga tggcgtcgga gacgcgctcg atgtggaggg tgatgcccca gccgtggagg
     5101 gggccctgct cgagggtggt gaggacgagc agatccaggg ttccctgcag cgtgtcagtc
     5161 tttttctgct tcacggtgct tccctataga cagctagggg agcatgcgcc cttagaagga
     5221 gagatgtcaa gaggcgggcg gagtttgtgg ccgtaagggt ttcaggaggg ggataccctg
     5281 gaattgctga acagtaacct gagtgaattc aggagcggcg gacgcggcgg gatgcggcgg
     5341 agattccggc ggcggcgatc actttgagga tttcgccggg aaggaagggc aggatcgaga
     5401 gggtgaagat ggcgg
//
LOCUS       DASZYN010000685         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1006271, whole genome shotgun sequence.
ACCESSION   DASZYN010000685 DASZYN010000000
VERSION     DASZYN010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006271"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="VHE33_17780"
     CDS             complement(<1..1346)
                     /locus_tag="VHE33_17780"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HVT99358.1"
                     /translation="MKKNAASLLLTFAFLVSPVLSQQQTYPFNDPNLPADKRIDNLLS
                     LMTVEEKIAVLGTQTGVERLGVPNIGSTEGIHGVVQREPRSGRQPITTTQFPQPPGMS
                     DTWDPELVRAAGDVEGYESRYITENPKYNRQILMDWGPQADLDRDPRWGRSEEVYGED
                     PFLNGTMAVAFAKGIEGNEKYWQASPLLKHFLANENENNRNGSSSNFDDRLFWEYYSL
                     PFRMAFQEAGASAVMASYNGWNGTAMAVNPILKTLVRDQWGVNVLSTDGGAVTLLVNP
                     RHAYPNHEAAVVACIKNGINQFLDKYADETNAALKDGSLTEADIDNVLRTKFRIALKL
                     GLLDAEGRSTFAHMKDAPEPWAANRDKDVSMKMALESVVLLKNEKNLLPLNKKKIKSI
                     AVIGPLADSVHWDWYGGTPPYKVTPLEGIRAEAGPNVKVNYAPGDTMAGEADAMKAA"
BASE COUNT          373 a          635 c          568 g          476 t
ORIGIN      
        1 gcggccttca tcgcgtcggc ttcgccggcc atcgtatcgc cgggcgcgta gttcaccttc
       61 acattgggcc cggcttcggc cctgattcct tccagtggcg tcaccttata aggaggcgtc
      121 ccgccgtacc agtcccagtg caccgaatcg gccagcgggc cgatcaccgc aatggatttg
      181 attttctttt tgttcagggg cagcaggttc ttctcgttct tcagcagcac gaccgattcc
      241 agggccatct tcatcgacac gtctttatcc cggttcgccg cccagggctc cggcgcatct
      301 ttcatgtgcg cgaaggtgga ccggccctca gcatcaagga gcccgagttt gagtgctatt
      361 cggaatttgg ttcggagaac gttgtcgatg tccgcctcgg tgagcgatcc atccttcagc
      421 gcagcgttgg tctcatccgc atacttgtcc aggaactgat tgatcccgtt tttgatgcat
      481 gccacaacgg cagcttcatg gtttggatac gcgtgtcgtg gattgacgag cagcgtgacg
      541 gctccgccat cggtcgagag cacattcaca ccccactggt cgcgcaccag ggttttgagg
      601 atgggattca cggccatggc cgtgccgttc cacccgttgt aactagccat cacagcgctc
      661 gcgccggcct cctgaaacgc catgcggaag ggcagagagt aatactccca gaacaggcga
      721 tcgtcgaaat tcgaggatga cccattgcgg ttgttctcgt tttcgttggc cagaaaatgt
      781 ttcagcagcg gcgatgcctg ccagtatttt tcattgccct caatgccctt tgcgaacgcg
      841 acggccatgg tcccgttgag gaacggatct tcgccgtaaa cctcttcgct ccggccccag
      901 cgcggatcgc gatcgagatc tgcctgcggg ccccaatcca ttaatatttg ccggttgtac
      961 ttcgggtttt cggtgatgta gcgcgattcg tagccttcca catcgccggc ggcgcgcacc
     1021 agttcgggat cccaggtatc gctcatgccc ggaggctgcg gaaactgtgt cgtggtgatc
     1081 ggctgccgcc cagagcgcgg ttcccgctgc acgactccgt ggatgccctc ggtgctgccg
     1141 atgtttggca cgcccagccg ctcgacgccg gtttgggtgc ccagcaccgc gatcttttcc
     1201 tcgaccgtca tcagcgagag gaggttgtcg atgcgtttgt ctgcgggcaa attcggatcg
     1261 ttgaacggat aggtttgttg ctgtgacaga acaggagaca cgagaaacgc gaaggtcagg
     1321 agcagggaag cagcattctt cttcatcaga gggcaggtcc acgttcggcc gctcgccgac
     1381 ggcggtccag gtcgtcgaaa gtcattcgtc ttcggcaacg aagcctgagc tggcgatgcg
     1441 actctgcaaa cgcaccaagc ctatcacctc tcctgcgctg gagaggcggc ggatcatgct
     1501 actcaaacgt tgcagttcta aggttttcgc ccagcgccaa ttttgcatct tttgcaaatt
     1561 acaacttgtc catcttcgac gcctccgaaa gataggcgaa taaaaggcaa acatcccacc
     1621 ttcgataggg tacggatttg gcctccaggg cttatgttcg gaattgagct gtcatgcacg
     1681 atccggtgaa gctcgttcac agccctcggt cggtcaggag ccccgagggc tcttttcttt
     1741 tttgctgact gagctgggct tccacactcg tcctgttgtc tgaatcgctc ggccaatcgt
     1801 cccctcaaaa ctccggtccg gaacgcctgc ggcgcactcg gtcacaaaag cgagacacaa
     1861 cccttcgtcg gctctcgccg catggctaga atgtgccgag ttttcgcgct cccacgaacc
     1921 catgatccgg ctcacattcg cccttttcac tctggcactc tccgcctccg ccctcgccca
     1981 aacttccaac tccgggactc cgcccaccgc catgttcaag ggtggtccgg cactaatggg
     2041 agtctcccag gg
//
LOCUS       DASZYN010000686         2665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_43098, whole genome shotgun sequence.
ACCESSION   DASZYN010000686 DASZYN010000000
VERSION     DASZYN010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43098"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1346)
                     /locus_tag="VHE33_17785"
     CDS             complement(join(175..1097,1097..1346))
                     /locus_tag="VHE33_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611554.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HVT99359.1"
                     /translation="MKRSRFTEEQIIAILKEQEAGMPTTEVCRRHGISGATFYKWKAK
                     FGGLEVSDARRLRQLEDENARLKRLLADAVLDNAMLKEIKRKKILTPAAKREAVAHLQ
                     QMFEVSQRRACEVLGIDRTMVRYVSCRPDDAGVRGRIRELAGERRRFGYRRLHWLLGR
                     EGISMNHKKFRRLYREERLQVRRRGGRKRALGTRSPMMIPQGVNQRWSLDFVADALSC
                     GRRFRIFAVVDDCTRECVRLIADTSISGARVARELDAAVFERRALPTMVVSDNGTELT
                     SMAILRWSKERSVDWHYIAPGKPQQNGFIESFNARLRDECLNETIFTSLAHARQVLAA
                     WRHDYNHNRPHSSLGNMTPAEAAARSAGNPGWGLAPNPAVAIAPNHGHQHGPRLYA"
     gene            1679..>2665
                     /locus_tag="VHE33_17790"
     CDS             1679..>2665
                     /locus_tag="VHE33_17790"
                     /inference="COORDINATES: protein motif:HMM:NF033577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HVT99360.1"
                     /translation="MEERMRFVLRRLDGEPMSDLCREFGISRKTGYKIFERYEQEGLH
                     ALSDRSRRPVRYAHQLPEPIERMIVRAKQDKPHWGARKIRELLVRRLHGDLRVPATST
                     IHAVMDRHGLVRRMRRRRHATGTPLSAGAQPNDLWCVDFKGEFKLARGPYCYPLTVTD
                     HASRYLLLCEALESVREQPAISAFEQLFQERGLPTAIRSDNGVPFASPNGLYNLSRLS
                     VWWLRLGIDIERIKPGCPQQNGRHERMHRTLKQEATRPAAGNFLQQQAKFDGFMKEYN
                     NERPHEALGMNVPAEVYTRSSRPYRGLPELEYPLHDRDVVVTCCGRICMHKKK"
BASE COUNT          495 a          886 c          800 g          484 t
ORIGIN      
        1 cccgtgcgca tggaaaacct gctttgcgat atccagacca atgatgctaa tctcacccac
       61 gggcgcctcc tctcagatgg ttggtcaaca ccaccatctt ggcacataga tgccgtaggg
      121 ggcgtccacc ccatcaccca aagcggactg gtctgagccc gaactttcta ccagttatgc
      181 gtagagcctc ggtccgtgtt ggtgcccatg attgggcgcg atggcaacag ccgggttggg
      241 ggcgagcccc caacccggat tgccggctga ccgggccgcc gcctctgccg gggtcatgtt
      301 gcccaggctc gagtggggcc ggttgtggtt gtaatcgtgg cgccaggcgg cgagcacctg
      361 gcgggcgtgg gcgaggctgg tgaagatcgt ctcgttgaga cattcgtccc gcagccgtgc
      421 attgaagctc tcgataaacc cgttctgctg cggcttgccc ggcgcgatat agtgccagtc
      481 gacggaccgt tccttcgacc atcgaaggat cgccatgctg gtcagctccg tgccattgtc
      541 gctgaccacc atcgttggaa gtgcgcgacg ctcgaacact gcggcgtcaa gctcgcgcgc
      601 cacccgcgca cccgagatcg acgtatcggc gatgagccgc acgcactcgc gggtgcagtc
      661 atcgaccacg gcgaagatcc ggaaccggcg tccacaggag agcgcatctg cgacgaagtc
      721 caacgaccag cgctggttga ccccttgcgg gatcatcatc ggcgagcgcg tgcccaatgc
      781 ccgcttgcgg ccgccgcggc ggcgtacctg cagccgctct tcgcgataca ggcgccggaa
      841 cttcttgtgg ttcatgctta tcccttcgcg tcccaacagc cagtgcaagc ggcgatagcc
      901 gaaccgacgc cgctcgccgg caagctcacg gatccggccg cgcacccccg catcgtcagg
      961 acggcagctg acgtagcgca ccatcgtccg atcgatcccc agaacctcac acgcccgccg
     1021 ctggctcacc tcgaacatct gctggagatg agccaccgcc tcgcgcttcg cagcgggcgt
     1081 cagaattttt ttgcgctgat ctccttcagc atcgcgttat cgagcaccgc gtccgcgagc
     1141 agccgcttca gccgcgcgtt ctcgtcttcg agctggcgga gccggcgcgc atccgacacc
     1201 tccagaccgc cgaacttcgc cttccacttg taaaaggtcg caccgctgat cccgtgccgc
     1261 cgacaaacct ccgtcgtcgg catcccagcc tcctgctcct tcaagatcgc aatgatctgc
     1321 tcttccgtaa acctgcttcg cttcactcgt ccgactcctc aatgggccgg actctactca
     1381 catctggagg aaattacggg gctcagacca gccggagaga atgccgctcg ggcgagacgc
     1441 caacccagtg accaaggtgc tctcttggca cgagcgtgtg atgctcgact gagcacaagt
     1501 gacggaactt gggcaagaat tgctgcccat gcgcgagtgc gcgcgtgaca ctctctcatc
     1561 acgtttgagc gtcccgacac ataggtgaca catcgttccg gacacatggg taacactttc
     1621 cccctggagc tgcagctcca ggggggaggt gttgatgccg tggaaggagt gttcggtgat
     1681 ggaagagcgc atgcgcttcg tcctgcggcg gctcgatggc gagccgatga gcgacctgtg
     1741 ccgagagttc ggcatctcgc ggaagaccgg ttacaagatt ttcgagcgct atgagcagga
     1801 gggcctgcac gcgctgagcg accggtcacg tcgcccggtg cgctatgccc atcagctgcc
     1861 cgagccgatc gagcggatga ttgtcagggc caagcaggac aagccgcatt ggggtgcgcg
     1921 caagatccgc gagttgctgg tgcggcggct gcacggcgac ctgcgagtgc cggcgaccag
     1981 caccatccac gcggtgatgg atcgccatgg gctggtcagg cggatgcgcc gaaggcggca
     2041 tgccactgga acgcccctgt cggctggtgc ccaaccgaac gatctctggt gtgtcgactt
     2101 caagggcgag ttcaagctcg ccaggggacc ctactgttat ccgctgaccg tcaccgatca
     2161 tgcctcgcgc tatctgctcc tgtgcgaagc actcgagtcg gtgcgcgaac aaccggcgat
     2221 ctccgcgttc gagcagctct tccaggaacg cgggctgccg acggcgatcc gctcggataa
     2281 tggcgttccg ttcgccagtc ccaacggcct ctacaacctg tctcgcttgt ccgtgtggtg
     2341 gctgcgcctc ggcatcgaca tcgaacggat caagccaggg tgcccgcaac agaacggccg
     2401 tcacgagcgc atgcaccgta cgctcaagca ggaggcgacc cgccccgcgg ctggcaactt
     2461 cctccagcag caagccaagt tcgatggctt catgaaagag tacaacaacg agcgcccgca
     2521 cgaagcttta ggaatgaacg tacccgctga agtctacacc cgatcgagcc gcccttatcg
     2581 cggcctgccc gagctcgaat acccgcttca cgaccgggat gtcgtcgtca cctgctgcgg
     2641 ccgcatctgc atgcacaaaa agaaa
//
LOCUS       DASZYN010000687         7450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1193484, whole genome shotgun sequence.
ACCESSION   DASZYN010000687 DASZYN010000000
VERSION     DASZYN010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7450
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193484"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="VHE33_17795"
     CDS             complement(<1..1189)
                     /locus_tag="VHE33_17795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT99361.1"
                     /translation="MKVLRTAGLALFLLTCLNLFNFIDRYVLPGVQPLIQREFRIDDA
                     RAGLLTSAFFFTYMIIAPLTGWLGDRLARKPLIVAGALIWSAATLLTAFVHDYQSLLI
                     RHAAVGVGEATFSIFAPALLADFYPEEARNKILSIFYITIPVGGALGYLLGGELGNHF
                     GWRVPFYVAAAPGVLIALCFWLFVREPKRGAADTLKATANRTSLRGLFRNPAYWTATL
                     GLAMWTFAVGGISTWLPTFLERFGGYSLAGADRLAGAVTVIDGLCATIAGGWLAQIWL
                     RRNHRALYLVSAWSSILAIPFAALVFFGPSRFLVAGAFLAEFSLFLSTGPINAAIVNS
                     VAAPIRSTAISVNLFVIHALGDSFSPGLIGHISDATNLRIGLGATLIALVLSGAILFG
                     GSRF"
     gene            complement(1275..1763)
                     /locus_tag="VHE33_17800"
     CDS             complement(1275..1763)
                     /locus_tag="VHE33_17800"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HVT99362.1"
                     /translation="MNDIGRLFQAFVAPAIFFSATALFILSINVRLMGIVSRLRQYVQ
                     ARHEAVKNGHTAEADACTAQIESMEQRAEMIRRTFLLSLVALVGTLISCLLLGFGLYW
                     KPASLAAAAVFVVAMIALLAGVLYYIREVTVALSSVRQEAEDERFEYPESQPELHEPS
                     IF"
     gene            complement(1842..2789)
                     /locus_tag="VHE33_17805"
     CDS             complement(1842..2789)
                     /locus_tag="VHE33_17805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HVT99363.1"
                     /translation="MSDIRFEAPRDPEPDHEIPPPPAEPPGYLWHPIERPAPLLELVP
                     PPPKPPKLIPNIGHVAVFLLLLIPALIGGYLVALFTIYAAHPHVRIPALTQEIAHNVM
                     YAISLQAAIYVVLWALAALVFTLWWDRSFAEGIHWNFSTALRSVLLFAGLGIISGLVI
                     TIAGNFLPMPKAPPILEDLTKSAAGAWALLIFGVTLAPLTEELAFRGFLLPSLINIFR
                     WFERRGSISQSTVRFVGIPLAILITSILFARMHAEQVSNSWGPVLLIGCVSVILCVVR
                     LRTDSVAAGVVVHICYNLTLFSGLLYQTDWFRHLDKLKT"
     gene            complement(2786..5662)
                     /gene="uvrA"
                     /locus_tag="VHE33_17810"
     CDS             complement(2786..5662)
                     /gene="uvrA"
                     /locus_tag="VHE33_17810"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HVT99364.1"
                     /translation="MAITQITVRGARQHNLQDINVKIPRNTLTVVTGLSGSGKSSLAF
                     DTIYAEGQRRYVETLSAYARQFLDQIERPDVDSIEGLSPAISIEQKTTSRSPRSTVGT
                     ITEIYDYLRLLYASVGVPHCPRCGRQISRQSAEQIVERILAFTPGERVTIYAPVVRGR
                     KGEFKDLLDKLDQQGFRARVDGEIQDLSDPPNLEKRKNHTIEAIVYRAVLKPGIQKAL
                     TESVEKALQMANGLVLVATQKDEQLYSSSMACPDCGLDIPKLEPRSFSFNSTYGACPE
                     CHGLGSIYDFDPAKIITDWSKPLLDGGLGPGSGSQYLIRLINIAAQKYKINLKLPFEQ
                     LTLKQQELLLVGPPPSEAPRTGFHGILAYLRDSLDEARSDTYREWMMQYMSASECSVC
                     HGQRLRPESLAVKIDGMSIADFTALSMERALERARGITFTAREALIAERLRKEIVERL
                     QFLNAVGLSYLSLNRSAATLSGGEGQRIRLATQIGSRLRGVLYVLDEPSIGLHQRDNQ
                     RLIQALESLRDLGNTVLVVEHDEETIRHADYVLDLGPGAGRLGGQVVAEGSPDAVMRT
                     PGSLTGRYLAGEISTLHRLVPRPLTGKWISVEGARSHNLRDITARFPIGIMTVVTGVS
                     GSGKSTLVNDILYRALAQELYGSREAPGEHGKIKGFDQIDKAIRIDQSPIGRTPRSNP
                     ATYTGVFTAIRDLFAMLPESRERGYKAGRFSFNVQGGRCEACQGEGQRRIEMNFLPDV
                     YVQCEVCNGRRYNQETLAVKFNGHSIADVLDLTIEDALPVLGDIPQARQKLQTLVDVG
                     LGYIHLGQSATTLSGGEAQRMKLARELSKRQTGRTLYLLDEPTTGLHFDDVRKLLEVL
                     HRLSDLGNTVIIIEHNLDVIRNADWIIDLGPEGGEGGGRIVGEGPPEEVARIPHSYTG
                     QFLRPFYSGSNGSVAGAGEKPPANGSRNRRTKSATREPGQETIQ"
     gene            5929..6117
                     /locus_tag="VHE33_17815"
     CDS             5929..6117
                     /locus_tag="VHE33_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99365.1"
                     /translation="MQDAVNTLMLACAALASLAFGVLLAHGICRAAFAKLFRHAGAVA
                     AQRARTQIAAPVSQPDPL"
     gene            complement(6198..7217)
                     /locus_tag="VHE33_17820"
     CDS             complement(6198..7217)
                     /locus_tag="VHE33_17820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HVT99366.1"
                     /translation="MPLPVSILDLVSMRPGEHPGTAIARSVDLAQHAESWGYRRFWLA
                     EHHSIAGIASSATSVLIGHIAAATRTIRAGSGGIMLPNHAPLVIAEQFGTLASIYPDR
                     IDLGLGRAPGGDFHTMRALRRDNHQTGDDFPALLEELRRYLGPERPGQTVRAIPGQNT
                     NVPITLLGSSDFSAQLAAHLGLPFAFAAHFAPDLLYAATRLYRQNFRPSEQMREPWLI
                     VGVPIVAAETDAAARRLFTTPQQRFLRLIRGQPVELLPPVDSMDAIWQDWEAAAVESK
                     LRAAIVGSHRTVKEGLERLASGTGANEVIAVTDTWEHTDRLASYRHLAEIAASVESRP
                     TAISM"
BASE COUNT         1577 a         2032 c         2495 g         1346 t
ORIGIN      
        1 cgaagcgaga accgccaaag aggattgcgc ccgagagtac gagcgcaatc agggtggcac
       61 cgagaccgat gcgcaggttg gtggcgtcgg agatgtggcc gatgaggcca ggagaaaaag
      121 aatcgcccag agcgtgaatg acaaagaggt tgacggagat ggcggtagag cgaatgggag
      181 cggcgacgga gttgacgatg gcggcgttga ttgggccggt gctgaggaag agactgaact
      241 cggcgagaaa tgctccggcg acgagaaagc gggagggtcc gaagaagacg agtgcagcaa
      301 aaggaatcgc caggatggag ctccatgcgg aaacaagata gagcgcacga tgattgcggc
      361 gaagccagat ctgggccagc catccccccg cgatggtggc acagagaccg tcaatgacgg
      421 tgaccgcccc ggcgaggcgg tcggccccgg cgagcgagta tccaccgaaa cgctcgagga
      481 aggtcggcag ccaggtggag atgcccccga cggcgaaggt ccacatggcc aggccgaggg
      541 ttgcggtcca gtaggcggga ttgcggaaga gtccccgcaa gctggtacgg ttggcggtcg
      601 ccttgagagt gtcggcagct ccgcgtttcg gttcgcgaac gaagagccag aagcaaagtg
      661 cgatcagaac gccgggcgca gcggcgacat aaaagggcac acgccagccg aagtggttgc
      721 caagctcccc gcccaggaga taaccgaggg ctccgccgac cggaatggtg atgtagaaga
      781 tgctgaggat tttgttgcgg gcttcttccg gatagaagtc ggcgaggagt gcgggcgcga
      841 agatcgagaa agtcgcctcg ccgacgccga cggcggcatg ccgaatgagc aggctttgat
      901 agtcgtggac aaaagccgtg agcagtgttg ccgcgctcca gatcagcgct cctgccacga
      961 tcaggggttt gcgggcgagg cggtcgccga gccagccggt gagcggcgca atgatcatgt
     1021 aggtgaagaa gaaagccgac gtgagcagac cggcgcgcgc gtcgtcgata cggaattcac
     1081 gctggatcag aggctgaacc ccaggcagca cgtagcgatc gatgaaatta aacaggttca
     1141 gacaggtgag aagaaagagg gcgaggccgg ccgtgcggag gactttcacg tactacaggg
     1201 tacagggctc aggtcacggg aacgtaagcc gggcatcggc tgtcggcgcg aaaccgctgg
     1261 atttgtctat cggctcagaa gatgctcggc tcgtggagtt ccggttgtga ttccggatac
     1321 tcgaatcgct cgtcctcggc ttcctgccgc acagaactta gcgcgacggt gacttcgcga
     1381 atgtaataga gaacgccggc caggagggca atcatcgcga caacgaaaac ggctgcagcg
     1441 gccagcgagg ccggcttcca gtaaaggcca aaaccaagca gcaggcagga aatcagggtg
     1501 ccgaccagcg cgacgaggct cagtaggaaa gtgcggcgga tcatctcggc gcgctgctcc
     1561 atcgattcga tctgcgcggt acaggcgtcg gcttccgcgg tgtggccatt ctttacggct
     1621 tcgtggcgcg cctggacgta ctgccgcagg cggctgacga tgcccatgag ccgcacattg
     1681 atggagagga tgaacagcgc tgtggcagag aaaaaaatgg ctggagccac aaaagcctga
     1741 aaaagacggc cgatgtcatt catcaggctc agggtagtgg cgagcttcag aaaaagatgt
     1801 gttcccgctc accgatctgt gtgcagaagg gcccgcaatg tctatgtttt cagtttatcc
     1861 aggtgccgga accagtcggt ctgatagagg aggccggaga aaagggtcag gttgtagcag
     1921 atgtggacga ccacgccagc agctacggag tccgtcctga ggcggacgac gcacaggatc
     1981 acactgacgc aaccgatgag aagcacgggc ccccacgaat tggacacctg ctcggcgtgc
     2041 atgcgtgcga acagaattga ggtaatcaaa atggcgaggg gaatgccgac gaagcggacg
     2101 gtggactgac tgatcgatcc gcggcgttcg aaccaccgaa agatgttgat gaggctgggg
     2161 aggaggaatc ctcggaaggc gagttcctcg gtcagcggcg caagcgtgac gccaaagatc
     2221 aacagagccc aggcgccggc agcggatttg gtcaggtctt cgaggattgg cggcgccttg
     2281 ggcatcggaa ggaaattgcc ggcaattgtg atgacgagac cggagataat tccgaggccg
     2341 gcgaaaagca gaacgctacg cagagcggtg gagaaattcc agtgaatacc ttccgcaaag
     2401 gagcgatccc accagagcgt gaagaccagc gcggccagcg cccacagcac gacatagatg
     2461 gccgcctgca gggaaatggc gtacatgacg ttgtgggcaa tttcctgtgt cagtgccggg
     2521 atacggacgt gggggtgcgc ggcgtagatg gtgaagagcg caacgagata gccaccgatc
     2581 agcgcaggaa tgagcagcag cagaaaaacc gcaacatggc cgatgttggg aatcagtttc
     2641 ggcggtttgg gcggtggcgg aaccagctcc agcagcggag ccggccgctc gatcgggtgc
     2701 cacagatagc cgggaggttc ggcgggcggc ggcgggattt catggtcagg ctccggatcg
     2761 cgcggagcct cgaaacggat gtcgctcatt gaatagtctc ttgccctggc tctcttgtgg
     2821 cggactttgt gcggcggttc cggcttccat tcgccggcgg tttttcccca gctccggcga
     2881 ctgatccgtt tgaaccagaa taaaagggcc gcagaaattg gccagtatac gaatggggga
     2941 tgcgggcgac ctcttcggga ggaccttcgc cgacaatgcg gccgccgcct tcgccccctt
     3001 cggggcccag atcgatgatc cagtcggcgt tacggatcac atcgagattg tgctcgatga
     3061 tgatgaccgt gttacccaga tcgctgaggc ggtgcaatac ctcgagcaac ttccggacat
     3121 cgtcgaagtg gaggccggtc gtcggctcgt caagcaggta gagtgtgcgg ccggtctggc
     3181 gcttggaaag ctcgcgggcg agcttcattc gttgggcttc gccgccggag agggtggtgg
     3241 ccgactgccc gagatggata tagccgaggc cgacgtcgac cagggtttgc agcttttgcc
     3301 gcgcctgtgg gatgtcaccg agaacgggaa gcgcatcttc aatcgtcaga tcgagcacat
     3361 cggcgatgga atgcccgttg aacttcacgg ccagggtctc ctgattgtaa cggcggccgt
     3421 tgcacacctc gcattgcacg tagacgtcag gcaaaaagtt catctcgatg cggcgctggc
     3481 cctcgccctg gcaggcctcg catcgtcctc cctgaacgtt gaagctgaaa cgcccggctt
     3541 tgtagccgcg ctcacgcgat tccggcaaca tggcgaacag gtcgcgaatg gcagtgaaga
     3601 cgccggtata ggtggctggg ttggagcgag gagtgcgccc gatcggcgac tggtcgatgc
     3661 ggatggcctt gtcgatctgg tcaaagcctt tgatcttgcc gtgctcgccg ggagcctccc
     3721 gcgatccgta gagttcctga gcgagcgcgc gatacaggat gtcgttgacc agagtgctct
     3781 tgccggagcc ggagacaccg gttacgacgg tcatgatgcc gatggggaac ctggcggtaa
     3841 tgtccctgag gttgtgactg cgcgcgcctt ccacgctgat ccactttcca gtgaggggac
     3901 gcggcacgag gcgatgcagt gtcgagattt ctccggccag atagcgcccg gtgagcgatc
     3961 ccggggtccg catgacggcg tcgggcgaac cttcggcgac gacctgaccg ccgaggcggc
     4021 cagctccagg ccccaggtcg agaacatagt cggcatggcg gatggtttct tcgtcgtgct
     4081 cgacaacgag aacggtatta ccgagatcgc gcagcgattc cagcgcctgg atcagccgct
     4141 ggttatctcg ctgatgcagc ccgatggagg gctcgtcaag aacgtaaagg acgccacgga
     4201 ggcgtgagcc gatctgcgtg gccaggcgga tacgctggcc ctctccgccg gaaagcgtag
     4261 ccgcgctgcg gttgagtgaa agatagctga ggcccacagc gttcaggaac tgcagtcgct
     4321 cgacgatttc cttgcgaagc cgttcggcga tgagggcctc gcgcgccgtg aacgtgatgc
     4381 cgcgggcgcg ttcgagggca cgctccatcg atagcgcagt gaaatccgcg atagacatgc
     4441 cgtcgatttt taccgccagc gattcgggac gcaggcgctg gccgtgacaa accgagcatt
     4501 ccgaagccga catgtactgc atcatccatt cgcggtaggt gtcggaacga gcctcgtcga
     4561 gcgaatcgcg aagataggca aggatgccat gaaagccggt gcgcggcgct tccgatggcg
     4621 gcggcccgac cagcagaagc tcctgctgtt tcagtgtgag ttgttcgaag ggcaacttga
     4681 gattgatttt gtacttctgc gcagcgatgt tgatcaggcg gataagatac tgcgaaccag
     4741 agcccgggcc aagtccacca tcgagcagcg gtttggacca gtcggtgatg atcttagccg
     4801 ggtcgaagtc gtagatactg ccgaggccgt gacactccgg gcaggcgccg taggtcgagt
     4861 taaaagagaa actgcgcggc tcaagcttcg gaatatcgag cccgcagtcg gggcatgcca
     4921 tggaggacga gtaaagctgc tcatcctttt gtgtggcgac caggacgaga ccgttcgcca
     4981 tctgcagggc cttctcgaca gattcggtga gagccttctg aatgccgggc ttgaggacgg
     5041 cgcgatagac gatagcttca atcgtatggt tcttacgttt ctcaaggttg ggcggatcgg
     5101 acagatcttg aatttcgcca tcaacccggg cgcggaaccc ctgctggtcg agcttgtcga
     5161 ggagatcctt aaattcccct ttgcggccac gcactacggg cgcgtagatc gtcactcgtt
     5221 cgccgggtgt aaaggcaagg atgcgctcaa caatctgctc ggctgactgc cgcgagatct
     5281 gccggccgca ccgcggacaa tgcgggactc caaccgaagc gtagagaagg cgcagataat
     5341 cgtagatttc ggtgatggtt cctacggtgc tgcgcggact gcggctggtc gttttttgct
     5401 cgatggagat ggccggcgaa agcccttcga ttgagtcgac gtccggacgc tcgatctgat
     5461 ctaaaaattg gcgggcatag gccgataggg tctccacgta ccggcgctgg ccctccgcgt
     5521 agatggtgtc gaacgcgagg gaggacttcc cggatccgga aagaccggtg acgacggtaa
     5581 gggtgttgcg cggaatcttg acgttaatgt cctgcaggtt gtgctggcgg gcgccgcgca
     5641 cggtgatttg agtgatggcc atagcacagg gggaatggat tgtaaatcgg aaactcgact
     5701 cagtgtagga gcatgcgaat agcacgctgg tactagttgc aaaacgggac gttccgtcta
     5761 gaattcggaa agttgatctc tttcgtatct tattcgccaa cggaaaagcg ggtcaacgtg
     5821 tcccatttct gttaggaatg gatcgtaaga atttgtcggg atgctgattt taagcatcca
     5881 atcaccggtt atgatccccc tcggcaggcg aggaatggaa ttgaggctat gcaggacgct
     5941 gtgaacacgt tgatgctggc ttgcgcggct ctcgcctcgc tggcttttgg agttctgctg
     6001 gcccacggca tttgccgcgc cgcctttgcg aagctcttcc ggcatgccgg agcagtcgcc
     6061 gcccagcgcg ccaggacgca gatcgccgcc ccggtctccc agcccgatcc gctttagctc
     6121 gccgcggttt tcttgcccgt tacctccagc ttttgccgca cgcaggaatg cgccgttttc
     6181 catcccggcc tgcgcgatca catgctgatt gccgtaggtc ttgattccac ggatgccgca
     6241 atctctgcta aatgccgata cgatgccagg cgatccgtat gctcccacgt gtccgtaacc
     6301 gcgatgactt cgttggctcc ggtccctgat gccagccgct cgagcccctc ttttacggtc
     6361 cgatgggagc cgacaatcgc ggcgcgaagc ttgctttcaa cggccgccgc ttcccagtcc
     6421 tgccagatcg cgtccatgga atcaacaggt ggcaacaact caaccggctg gcctcggatt
     6481 agccgcaaaa aacgctgctg gggagtcgtg aagaggcgtc gcgctgctgc atcggtttcg
     6541 gcagccacga tcggcacgcc gacgatcagc cacggctccc gcatctgctc actcggcctg
     6601 aagttctggc ggtagagccg cgtagcggcg tagaggagat ctggagcgaa atgcgccgcg
     6661 aatgcaaagg gcaaaccaag gtgggctgcc aactgggcgc tgaagtcact cgaccccagc
     6721 aaagtgatcg ggacattcgt gttctgtccc ggaatggctc tgacggtctg gccgggacgt
     6781 tcgggcccca aatatcgacg cagctcttcg agaagggcgg ggaaatcgtc gccggtttga
     6841 tgattatcgc ggcggagggc acgcatcgta tggaaatcgc cgccgggtgc gcggccaaga
     6901 cccagatcga tgcggtccgg ataaatggag gcgagtgtgc caaattgctc ggcaatgacc
     6961 agcggcgcgt gattgggcag catgatgccg ccactgccgg cgcgaatggt ccgggtggcg
     7021 gcagcgatgt ggccgatgag aactgaagtt gccgaagagg caataccggc gatggagtgg
     7081 tgctcggcca gccaaaaacg ccgatatccc cacgactcgg catgctgcgc gagatcgacg
     7141 gagcgtgcga tggccgtgcc cgggtgctcg ccggggcgca tcgagaccag gtcgaggatc
     7201 gataccggca gaggcacgtt gcgttagatg aatcaaccgg catgaagggt gcgggctgat
     7261 agctgcgtcc acaggagcct gcacttagca tcgcggctat tggtcgcgaa tcgcctccat
     7321 gggatcgaca gacgccgctc gccctgcggg aaagacaaac gccagcacca gaacagccat
     7381 cagcaccgca aaagtggcca gcgcgaccag ggggcttccc ggggtgacgc cgtacagcaa
     7441 tgactggagg
//
LOCUS       DASZYN010000688         8922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_295313, whole genome shotgun sequence.
ACCESSION   DASZYN010000688 DASZYN010000000
VERSION     DASZYN010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8922
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295313"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1991
                     /locus_tag="VHE33_17825"
     CDS             <1..1991
                     /locus_tag="VHE33_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99367.1"
                     /translation="PSQPRVIYAANDNLWRSRDDGETWTMVWPPPSSVQGISMASDHS
                     DEKVLSRENPLDTITALVIDPRDSNHFYASAGGKSPGLFESADAGARWTRIADLPELA
                     NRLWFRSKPSASLLAAGQQFVAEWSGHKLRVSSPPAPLLDSSSAMTKEGGWILYGATE
                     DGITVSRDDGATWIKASLPGTGAKVRAIATSLHHPDVAYASYSGLHLDDSVWHGVART
                     DDAGKSWKLVWKEAETAAPNVHDAWITQDFGTDWGENPLNLTTADQDSNIVYGTDFGR
                     ALISTDGGRQWHAAYSRSTPDGGWISTGLDVTTSYGIHFDPFDVRRRFITYTDISLFR
                     SETGGKSWLSSSKGVPKNWMNTAYWMVFDPAVKGRAWAVYSGTHDLPRPKMWRHTSTD
                     RYRGGVGRSDDGGRTWNPSSTGMPQTAATDIVLDPSSPSGRRTLYVTAFGRGVYKSTD
                     DGRTWSLRNTGIRQNTPFAWRITLAPDHVLYLVVARRSEDGSIGNSGDGAIYRSSDAG
                     ETWQPLPLPDGVNGPTGIAVDPAHPQRLYLSAWARAVGTHGEGGGVYVSDDGGQHWAP
                     SLSKDQHIYDVTPDPRLPGTIYACGFESSAWVSRDAGIHWQRISGFNFKWGHRVVLDP
                     EDSHMIYITTFGGGVWHGQVTTDPAILDIATPEMEPGR"
     gene            1988..5212
                     /locus_tag="VHE33_17830"
     CDS             1988..5212
                     /locus_tag="VHE33_17830"
                     /inference="COORDINATES: protein motif:HMM:NF028438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HVT99368.1"
                     /translation="MKKRVLCSLAALALSTCALPAQVKVYQGTLSLPYYQEGPPNPNP
                     PFDEYAANRFNYPYVLRDNLTSHRVERAFRAVYLENEYLRCAILPDVGGHIYTCTDKI
                     NNVPMFYQNPSIKEAAIGMRGGWAAFGLEFNFPVSHNWMTVSPIDFAYGKEQDGSAWV
                     TVGNVDRVYGMEWTVRIVLHPGSTLLQLQVTLNNRSDVRHRFYWWSNGGIQAWDDTRI
                     CYPMRYTAAHGFADVDRWPVDSSGTDLSILRNHTHGPVSRFVYGSREPFMGIWNPHTN
                     AGVAHYTDYAELPAKKIWSWGVDADGLDWRKALSDNDSAYVEVQGGLFRNQETYAFLQ
                     PRQAIHFTEYWMPLHELGGLARANLAGAVNLSRAGSMLIAALNVTTKIPQAQIRLLDG
                     DRALFSTTLDLAPEHTWKHEIAIADPAHHYTIEVRDHSGQVLIAQTEGKYDWTPDSEI
                     KVGPQKHVTFPPAAQRSAEDWSRIGYDDELNGRLLIAARDYEEGLKAFPQSQALAVDA
                     GRLDTALLQFDHAIPLLKAAEARDTPNAEIAYYLGLAYDGLNDTRHAQTAYETAARLP
                     QMRAAAALRLGELAARTGDLQTANTWLADALHSAPDDLRTAEELAAILHARGKNADAH
                     TLAATWYSRYPTSYFLAEELGTPQTEHLAADPERVLNVASEYMRLGRYESALHVLSRN
                     YPHVDAGEVEPGSLPPGQNPLVAYYAAYCLQKLGRPFAEEASRASHMPLQYVFPSGMQ
                     TAAVLEASLEKNADDATAHYLLGLLQFSTGMTESAEQHWNRAHDLHAVIPGLDADRGL
                     ARLHIDSDPQGALAAFREGLRTDASNEAIYVGIDQALSILGLPASEFIAAMEQYPDKP
                     KMPARLVYELALHQAEAQNFSAAQDLLRNRFFPREEGGTNVREVWIEIELQRLQSLAA
                     NGKCEEAAAGLSKLSQPVPGMDFTKDGLTAFIESSHSQYEVAMIDRACKQTDEANAIL
                     GKIAANTSGEEVVWAAQAARLLPGYDQAAWQTRLSAALAHADPDALTNSYQAYIVGML
                     QQEAGASDAAKATFRKALLLPDQTLSYHLTRMAIADSGNQTPVP"
     gene            complement(5261..5965)
                     /locus_tag="VHE33_17835"
     CDS             complement(5261..5965)
                     /locus_tag="VHE33_17835"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HVT99369.1"
                     /translation="MKKIILACMLMVADLASAQASNPAEFRSVFAPHDVTLQTDSASP
                     FWRDAIPVFAERDNYGHPVPHHRTEIRSRWTERNLYLLFVCPYDELHLRPHPSTAAET
                     NQLWNWDVAELFIGSDWKNIRRYREFEVSPQAEWVDLDINLDLPDHTVGWTWNSGFHV
                     AASIDRGAKVWYAAMVIPLASIGNEPPHAGLAYRANLFRSQGPPERRRSICWRPPMRE
                     TFHTPESFGKLVLVEK"
     gene            complement(6038..7258)
                     /locus_tag="VHE33_17840"
     CDS             complement(6038..7258)
                     /locus_tag="VHE33_17840"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVT99370.1"
                     /translation="MNRRELLIGTGAGMMAGTVGVAGAAGKAGLSESAEPKKAHRNLY
                     LSEYQPKSMLQVKETHVARSRYPVIDIHTHITDAARAKNGVSLGPERQYLGTPAELLQ
                     VMDAKNIRSLVNLTGGYETGLAETVQRYDRAYPDRFYTFTEPSYSRFREPDYPRMQAL
                     AIEQAHRNGARGLKILKTLGLYLRENITSGELVKIDDPRFDPMWDACGQLNIPVAIHV
                     SDPVAFFTPTDRFNERYEELNNHPDWSFYGPSFPSNAELLEARNRVIARHPKTRFIVL
                     HVGNFAEDLGNVSENMDRFPNMNVDIAARIGELGRQPRTARAFFEKYQDRILFGTDAT
                     PHGDDYPQQVFNDKLYEIYYRFLETEDEYFDYAPADIPPQGRWRIYGVGLPDDILRKV
                     YHANAARELHLSIA"
     gene            7544..8899
                     /locus_tag="VHE33_17845"
     CDS             7544..8899
                     /locus_tag="VHE33_17845"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HVT99371.1"
                     /translation="MSTSSGAQLQRSLKLWNLVLYGIIIIQPTAPMGIYGVVNNIAHG
                     HVTTTVFIAMCAMLLTAVSYGRMARVYPQAGSAFSYVSEELHPRLGYLVGWAMLMDYI
                     LNPVICIIWCSQAARNVAPDVPYWVWVTAFALLSTFLNTRGIQTSSRVNAVLAVFMSV
                     VVVLFLGYGVHYILTLVHPTTTDLVRPLYDRSTFAASAVFHGTSVAVLTYIGFDGIST
                     MAEEVENPRRNILIATVLTCFIIGLLSMAQVYVAQLAWPGRDPFPPSMVDTAFVHVAD
                     RVGGRFLFQLLNATLLIANMGSAIAAQFGASRLLYGMGRSRSLPAKFFAAIDAKYHVP
                     RNNVLLIGAIALIGPLFLTYEKGAELLNFGAFIAFIGVNAAALLHYKYRSKERVAFPF
                     LCPALAIVVCLFIWWNLSVSARLLGTIWLVVGVLVALAMHKQTRLARGLTDEQDGPPP
                     A"
BASE COUNT         1822 a         2766 c         2537 g         1797 t
ORIGIN      
        1 acccttctca gccacgcgta atctatgcgg cgaacgacaa tctgtggcgg agccgcgacg
       61 acggtgagac ctggactatg gtatggcctc ccccttcgag cgttcagggc atctccatgg
      121 cctccgatca cagcgacgaa aaagtcctct cccgcgaaaa ccctctcgat acgatcactg
      181 ccctggtgat cgatccgcgc gactcgaatc acttctatgc ttcggcgggg gggaagagtc
      241 ccggactctt cgaatcggct gacgcgggcg caaggtggac ccgcatcgcc gacttgccgg
      301 agctggcgaa ccggctctgg ttccggtcga aaccctctgc atctttgctg gccgcgggcc
      361 agcaattcgt cgcggagtgg tcgggtcaca agcttcgggt atcttcccct ccggcccctc
      421 ttctggactc ctcctcggca atgacaaaag aaggaggctg gatcctctac ggcgcgactg
      481 aggatgggat cactgtttcc cgcgacgatg gcgcaacgtg gatcaaggcc tccctgccgg
      541 gaaccggggc aaaggtccgt gcaatcgcaa cgagcctcca tcaccctgac gtcgcgtatg
      601 cctcctacag cggccttcac ctcgacgatt ctgtctggca tggcgttgcg cgaaccgacg
      661 acgccggcaa gtcatggaag ctggtgtgga aggaagctga gactgccgcc ccaaacgtcc
      721 acgatgcatg gattacgcag gacttcggca cggactgggg ggaaaaccct ctcaacctga
      781 cgactgccga tcaggattcc aacattgttt acggaaccga tttcggtcga gccctgatca
      841 gcacggacgg cggcaggcaa tggcacgccg cgtattcgcg cagcaccccg gacggtggct
      901 ggatcagtac agggctggac gtgacaacct cttacggcat tcacttcgat cccttcgatg
      961 tccggcggcg gttcatcact tacaccgata tttcgttgtt ccgcagcgag acgggtggaa
     1021 agtcgtggct gagttccagt aaaggtgtgc caaaaaactg gatgaatacc gcctactgga
     1081 tggtgttcga tcccgcggta aaaggacgcg cctgggccgt gtacagcggc acgcatgacc
     1141 ttccccggcc gaagatgtgg cggcacacct cgaccgatcg ctaccgcggc ggcgttggcc
     1201 gcagcgatga cggcggacgg acgtggaatc cctccagcac gggaatgcca cagactgcgg
     1261 ccacagatat cgtcctcgat ccatcgagtc cgtccgggcg ccgcactcta tatgtcaccg
     1321 catttggaag aggcgtctac aaaagcaccg atgatgggcg aacctggtcg ctcaggaata
     1381 caggaatccg gcagaacaca ccgttcgcct ggcgaatcac gcttgcgccg gaccacgttc
     1441 tttatctggt cgtggcgcgg cggtcggagg acggcagcat tggcaacagt ggagacggcg
     1501 cgatctatcg gtcttcggat gccggcgaaa cctggcagcc attgcctttg cccgatggag
     1561 tcaacggccc cacgggcatc gccgtggatc ccgctcatcc gcagcgcctt tatctttcgg
     1621 catgggctcg tgcagtgggc acacacggag aaggcggcgg tgtgtatgtc tccgatgacg
     1681 gcggacagca ctgggctccg tcgctcagca aggaccagca tatttacgat gtcacgccgg
     1741 atccgcgcct gccgggcacg atctatgcgt gtggatttga atcctctgcc tgggtatcgc
     1801 gcgacgcagg catccactgg cagaggatct ccggtttcaa tttcaaatgg ggacatcgcg
     1861 tagtcctcga ccccgaagat tcacacatga tctacatcac cactttcggc ggaggcgtgt
     1921 ggcatgggca ggtgaccacc gatcctgcaa tcctggacat tgccacacca gaaatggagc
     1981 ccggtcgatg aagaagcgtg ttctctgcag tcttgccgct ctcgctctct ccacctgcgc
     2041 tttgcccgcc caggtgaagg tttaccaggg tacgttgtct cttccttatt accaggaagg
     2101 gccgcctaac ccgaatccgc ccttcgacga gtacgcggcg aaccgattca actaccccta
     2161 cgtcctgcgc gacaatctca cctcgcatcg ggtcgaacgc gcgttccgcg ccgtctatct
     2221 ggagaacgaa tatctccggt gcgcgattct gcccgacgtg ggcggccaca tttacacctg
     2281 caccgacaag atcaataacg ttccgatgtt ctaccagaat ccgtccatca aagaagccgc
     2341 catcggcatg cgcggcggat gggcagcctt tggactggaa tttaattttc ccgtctcgca
     2401 taactggatg accgtctccc caatcgactt cgcctacggg aaagagcagg acggcagcgc
     2461 ctgggtcacg gtcggcaatg tcgaccgcgt gtacggaatg gagtggacgg tccggatcgt
     2521 gcttcatccc ggcagcactc tgctccagtt gcaggtcacg ctcaataacc gcagcgacgt
     2581 gcggcatcgc ttctactggt ggagcaatgg cgggattcag gcgtgggacg acacgcgcat
     2641 ctgctacccc atgcgctaca ccgcggccca tggattcgcc gatgtcgatc gctggcccgt
     2701 ggactcaagc ggtaccgatc tgagcatcct caggaaccat acccacggtc cggtttcgcg
     2761 attcgtgtat ggcagccgcg agcctttcat gggaatctgg aatccccaca ccaacgccgg
     2821 agtggcgcac tacaccgact atgcggaact gcccgccaag aagatctggt cgtggggcgt
     2881 ggatgccgat gggctggact ggcgcaaagc actctccgac aatgacagcg cgtatgtcga
     2941 agtgcagggc ggcctcttcc gcaatcagga gacttacgca tttctgcagc cgcgccaggc
     3001 cattcacttc acggaatact ggatgccgtt gcatgagctg ggaggactcg ctcgcgccaa
     3061 cctggcgggt gcggtcaacc tgagccgagc gggcagcatg ctgatcgcgg ccctgaacgt
     3121 aaccacgaag attccgcagg cacaaattcg actgctcgat ggcgaccgcg ccctgttcag
     3181 caccactctc gatctcgctc cggaacacac ctggaagcat gagatcgcaa tcgccgatcc
     3241 ggctcaccac tacacaatcg aagtacggga tcacagcggc caggttctca tcgcgcagac
     3301 ggaaggcaag tatgactgga cgcccgattc ggaaatcaag gtgggaccgc agaaacatgt
     3361 cacctttcca ccagcagcgc agcgcagcgc cgaggactgg agccggatcg gctacgacga
     3421 tgagctgaac ggccgcttgc taattgctgc ccgggactac gaagagggat tgaaagcctt
     3481 tccgcagagt caggcgctgg cggtcgatgc cggtcgcctg gacaccgcgt tgctgcagtt
     3541 cgaccacgcc attcctttgt tgaaggctgc cgaagcgcgc gacacgccca acgccgagat
     3601 cgcttattac cttggcctgg cctacgacgg cttgaacgat actcgtcacg cacaaactgc
     3661 gtacgaaacc gcggctcgac tgccgcaaat gcgcgccgct gctgcactcc ggttaggcga
     3721 gctggccgcg cgaacgggcg atctgcagac ggcaaacacg tggcttgcgg acgcactgca
     3781 cagcgctccc gacgatctgc gcaccgcgga agaacttgcc gccattcttc acgctcgcgg
     3841 caaaaacgct gacgcgcata cgctggctgc aacctggtac agccgctatc cgaccagcta
     3901 ctttctggcc gaagagctgg gaacgccgca gaccgagcac ctggcggccg atccggaacg
     3961 tgtcttgaac gtcgcatcgg agtacatgcg gctgggacga tacgaatcgg ctcttcacgt
     4021 tctctcgcgc aactatccgc acgtggacgc aggagaagtc gagcccggtt cccttccccc
     4081 cggacagaat cccctcgtgg cgtattacgc ggcctattgc ttgcagaagc tcggccgtcc
     4141 gttcgccgaa gaagcatcgc gcgcttctca catgcccttg cagtatgtgt tccccagtgg
     4201 catgcagacc gccgcggtgc tggaagcgag ccttgagaag aatgccgacg atgcgaccgc
     4261 ccactatttg ctcggcctgc tgcagttttc gacagggatg accgaatccg ccgaacaaca
     4321 ctggaaccgg gcacacgatc tgcacgccgt cattcctgga ctcgacgccg atcgcggcct
     4381 ggcccgtctg cacatcgatt ccgatcccca gggcgcgctg gcggcctttc gcgaagggct
     4441 caggaccgat gcctccaacg aggccatcta cgtcgggata gaccaggcgc tcagcattct
     4501 ggggcttccc gcatccgagt tcatcgctgc aatggaacag tatcccgaca agccgaaaat
     4561 gcccgccagg ctcgtttacg aactggctct gcaccaggcc gaagcccaaa atttctccgc
     4621 cgcccaggac ctgctgcgca acagattctt cccgcgcgaa gaaggcggca ccaacgtgcg
     4681 tgaggtttgg atcgagatag agttgcaaag actccagtcg ctcgcggcga acggaaagtg
     4741 tgaggaagct gcggccggcc tgtccaaatt gagccagcct gtgccgggaa tggacttcac
     4801 caaagatggc ctgacagcct tcatcgaatc aagccactct cagtacgaag tcgcgatgat
     4861 cgatcgcgcg tgcaagcaaa ccgatgaagc caacgccatc ctcgggaaga ttgccgccaa
     4921 taccagcggc gaggaagtgg tgtgggcagc tcaagcggct cgccttctgc ctggatacga
     4981 ccaggcagca tggcaaacgc gcctctccgc tgccctggcc cacgcggatc ctgacgcgct
     5041 gacaaactcg taccaggctt acattgtcgg aatgctgcag caggaggccg gagcgagtga
     5101 tgccgcaaag gcaacgttcc ggaaagcgct tctgctgccg gatcagacgc tttcgtacca
     5161 cctcacccgt atggcgatcg cggactccgg gaatcaaacg cctgtgccgt gacttgcgtg
     5221 aagcgaactc ttcacgacgg gaacagttcg gcaccgccgc ctacttttcg accagcacca
     5281 gcttgccgaa gctctccggc gtgtggaacg tctcgcgcat cggcggcctc cagcagatcg
     5341 atcggcgccg ttccggcggt ccctggctgc gaaagagatt ggcacgatag gcgagtccgg
     5401 catgcggagg ctcgttgcca atcgacgcca gcggaatgac catcgccgca taccagacct
     5461 ttgccccgcg atcgatgctg gccgcaacgt gaaagccgga attccacgtc caccccaccg
     5521 tgtgatcggg gagatcgagg ttaatgtcca gatcgaccca ctcggcctga ggagatactt
     5581 caaactccct ataccggcgg atgtttttcc agtcggagcc aatgaagagt tcagcgacat
     5641 cccagttcca aagctgattg gtttcggcgg ccgtgctggg atgcgggcgc agatggagtt
     5701 cgtcataggg acagacgaac aacaaataga gattgcgttc cgtccatcgc gagcggatct
     5761 cggtgcgatg gtgaggcacc ggatgaccgt agttgtcccg ctctgcgaac acgggaatcg
     5821 cgtcccgcca gaacggcgac gcagaatcgg tctggagagt cacatcgtgc ggcgcgaaga
     5881 cgctgcggaa ctccgccgga ttcgatgctt gagcggaagc caggtcagcg accatcagca
     5941 tgcacgcaag gataattttc ttcacaggcc atcaccggaa tgtgtttccc cgttcgttgc
     6001 cccgttcgcg aacgacgcaa ttactccgct cagccggtca tgcgatcgac aagtgcagtt
     6061 cgcgcgccgc gttggcatgg tagaccttcc gcaggatgtc atcgggtaaa ccgacgccgt
     6121 agatgcgcca gcgcccctgc ggcggaatat cagcgggtgc gtagtcgaag tactcgtctt
     6181 ccgtttcgag aaaacgatag taaatctcat acagcttgtc gttgaagacc tgctgcggat
     6241 aatcgtcgcc gtgaggcgtg gcgtcggtcc cgaagagaat gcggtcctga tacttctcga
     6301 aaaaagcacg cgcggttctc ggttgccgtc ccagttcccc gattcgcgcc gcaatatcga
     6361 cgttcatgtt cggaaagcgg tccatgtttt cgctcacgtt gccaagatct tcagcgaaat
     6421 tgcccacgtg gagcacgatg aaccgtgtct tcggatgccg ggcgataacg cggttgcgtg
     6481 cctccaggag ttcggcattg ctcgggaacg acggcccgta aaaggaccag tcgggatggt
     6541 tgttcagctc ctcataccgc tcattgaagc ggtcagtagg cgtgaagaac gcaaccggat
     6601 ccgaaacatg gatcgcaacc ggaatgttga gctgcccgca tgcatcccac atcggatcaa
     6661 atcgtggatc gtcgatcttc accagctcgc cgctggtgat gttttcacgc aggtacagac
     6721 cgagtgtctt caggatcttg agtccccgcg ctccattgcg gtgagcctgc tcaatcgcga
     6781 gagcctgcat gcgagggtag tcgggctccc tgaacctcga gtaagagggc tccgtgaatg
     6841 tatagaaacg gtcgggatag gctctgtcgt acctctgtac ggtctctgcc agacctgtct
     6901 cgtagcctcc ggtcagattc accagcgatc gaatattctt cgcgtccatc acctgcagca
     6961 gttccgctgg cgtgcccaga tactgccgct ctggtcccag cgatacgcca ttcttcgcgc
     7021 gagcagcatc cgtaatgtgc gtgtggatgt cgatcaccgg atagcgagaa cgcgccacgt
     7081 gcgtttcttt cacctggagc atgctcttcg gctggtattc ggagagataa aggttgcggt
     7141 gggctttctt cggctcggcg gactcagaca ggcctgcttt cccggccgca ccggccaccc
     7201 caaccgttcc tgccatcatt cccgccccag ttccgatgag caattccctg cgattcatcg
     7261 agcaaaatcc cccaatcatc cgaaagcaca gaaacaaata cgaaataaaa gtacagaaca
     7321 caaaaccatt ttgcaagcag ccccattcct ctgtaatctc acgtaaatat aagaaaacaa
     7381 tgtagagtct gattaattcc aggcaatcga ccaggatgac cctttcggta tttttttgtt
     7441 tcgtgtagaa attggatcaa ccagggcttt cgataccact ccgcatcctt ccgcgtcctg
     7501 aatttcacat cgaaggaccg cggcacacga acggaaaggc caagtgagta cttccagcgg
     7561 cgcacaacta cagcgttccc taaagctctg gaacctggtg ctgtacggca tcatcatcat
     7621 tcagccgaca gctcccatgg gcatttacgg cgtggtgaac aacatcgcgc acggccacgt
     7681 caccaccacc gttttcatcg ccatgtgcgc catgctgctg acagccgtca gttacgggcg
     7741 catggcgcgc gtttaccccc aggccggctc cgcgttcagc tatgtgtcgg aagaactcca
     7801 cccccgtctc ggctatctgg tgggctgggc aatgctgatg gactacattc tgaatccggt
     7861 gatctgcatc atctggtgca gccaggcggc tcgtaacgtt gcgcctgacg tgccctactg
     7921 ggtttgggtc acagccttcg cccttctctc cacatttttg aatacgcgtg gaattcaaac
     7981 ctcaagccgt gtgaacgccg tcctcgcagt cttcatgagc gtggtggttg ttctgtttct
     8041 gggctacggc gttcactaca tcctgaccct tgtccatccc accacaaccg acctggtccg
     8101 tcccctttac gaccgctcca ccttcgcagc ctcggcggtt ttccacggaa catccgtggc
     8161 ggtcctcacc tacatcgggt tcgacggaat ctccacgatg gcggaagaag ttgaaaatcc
     8221 gcgccgaaat attctcattg ccaccgttct cacgtgcttc attatcggat tgctttccat
     8281 ggcgcaggtc tatgtggcgc aacttgcctg gccggggcgt gatccttttc cacccagcat
     8341 ggtggataca gccttcgtgc acgtcgccga ccgggttggc ggacgtttcc tgtttcagct
     8401 tctgaacgca accctcctga tcgcgaacat gggatcggcc atcgccgccc agtttggggc
     8461 ctcgcgcctg ctgtacggca tgggcagaag cagatctctg cccgcgaagt tcttcgcggc
     8521 gatcgatgcg aagtaccatg ttccccgcaa taacgtgctc ctgattggcg caatcgcgct
     8581 catcggcccg ctctttctga cctatgagaa aggtgcagaa ctgctcaatt ttggagcctt
     8641 catcgccttt attggcgtga acgccgcggc gttactgcac tataagtacc gctcaaagga
     8701 gcgcgtggcg tttccgtttc tctgtcccgc gctcgccatc gtcgtctgcc tgtttatctg
     8761 gtggaatctg agcgtaagtg cccggctcct gggaaccatc tggctcgtcg tcggtgtcct
     8821 ggtggctctc gcgatgcata agcaaacacg actcgcgcgt gggctaaccg atgagcagga
     8881 tggtccacct cctgcctgat ctttcgaggt ccgttcaatg aa
//
LOCUS       DASZYN010000689         6555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_678453, whole genome shotgun sequence.
ACCESSION   DASZYN010000689 DASZYN010000000
VERSION     DASZYN010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6555
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678453"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..432
                     /locus_tag="VHE33_17850"
     CDS             46..432
                     /locus_tag="VHE33_17850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT99372.1"
                     /translation="MQVLIVEDQPSDLRVATTAAQESGFADIEARSSIAAARDFLQKA
                     LDEGRSLPDAIVLDLDLGYESGFELLRYWHSNPQLSKVSVVVWTILGNQYREICEMFK
                     VTAYLDKGAGGAALRNTLAGLKRLAS"
     gene            599..1498
                     /locus_tag="VHE33_17855"
     CDS             599..1498
                     /locus_tag="VHE33_17855"
                     /inference="COORDINATES: protein motif:HMM:NF025719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVT99373.1"
                     /translation="MTDLAEIISRNEDQIRSEWIRDMGKSVQRADLISKTELEEQCGA
                     LLRAVVAGAKSGDATDIDNPGWRQAREFLESVSASRARQGFSPTEVATFVLSLKQPLF
                     SAIRRDLSKNPDKLFDTVWAATEMLDGLALVTTGAYIATREELIVRQQQELMELSTPV
                     VKLWEGVLALPIIGTLDSARTQVVMENLLQTVVATNSRFAIIDITGVPTVDTLVAQHL
                     LKTITAARLMGAECIISGVRPQIAQTIVHLGINLEDVMTKANLADAFALALQMSGRTV
                     VRRSTASAETKGILAGPMTKQEN"
     gene            1500..1895
                     /locus_tag="VHE33_17860"
     CDS             1500..1895
                     /locus_tag="VHE33_17860"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVT99374.1"
                     /translation="MERIPVLRMGSLLLITIQVDMHDRLALQLQDDVTNRIAEWGSRG
                     VLIDISSLEIVDSFIGRMLANIAAMSRVLGAETVVVGMQPAVAITLVELGMSLPGVRT
                     ALSVEAGMELLYGAGAGFRTGSDDERDEA"
     gene            1876..2280
                     /locus_tag="VHE33_17865"
     CDS             1876..2280
                     /locus_tag="VHE33_17865"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HVT99375.1"
                     /translation="MSETKRETLPLTNSNDVVLARQKVRQWATEMRFTLVDQTKLVTA
                     ASELARNTLDHGKGGSMTIEQIAAGPRVGLRLIFEDRGPGIPDIELALKDGFTTGNGM
                     GLGLGGSKRLVNEFSIDSAPGKGTRITVVRWK"
     gene            2271..3263
                     /locus_tag="VHE33_17870"
     CDS             2271..3263
                     /locus_tag="VHE33_17870"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HVT99376.1"
                     /translation="MEVAFTEWIPITDESSVGEARRRALLVAQRIGFDQTRSGELALL
                     ATESSRNVLLHGRGGETVILGMQAQGKSLARILAMDNGPGIANLAQAMTDGYSTAGTM
                     GAGMGAMQRMATSMEVFTGKKGTVLLLELGESSPPGSLRIAGMAVPHPGERFCGDAWA
                     CHETPERSLILLADGLGHGRDAAEAAQEATAAFHKRQDHAPAEILGYVHDALKKTRGA
                     VAAIAEIRPIEATLSYAGVGNISAAVLTAAGSRSLVSHNGTLGMVTSRIQEFRIPWTP
                     DALLVLHSDGLQSKWDLSAYPGLITRHPAVIGGTLVRDFRRQRDDASVVVVKAA"
     gene            3265..5418
                     /locus_tag="VHE33_17875"
     CDS             3265..5418
                     /locus_tag="VHE33_17875"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT99377.1"
                     /translation="MAIPVFHLPLRTERDVVQARQRAREVAAELGLDNQDQIRMATAA
                     SEIARNAFRYARNGAVEFAVGLEDPQHLEITVSDTGPGIANLDEILDGRYKSTTGMGI
                     GLLGTRRLMDRFEIDSQPAGTTVRMVKHLPQQHASMTQRAVRELNAKLKQRTPESPYE
                     EIEQQNQELLKTLQELRSRQEELQLLNRELEDTNRGVVALYAELDERADYLRRASELK
                     TKFLSNVSHEFRTPLNSIISLARLLMDRIDGDLNSEQTKQVRFIESSARDLQEMVNDL
                     LDLAKVEAGKIRIRPKHFQVHELFSALKGMLKPLLADNTSVDLIFEDATELPALHTDE
                     GKVSQILRNLISNALKFTPAGHVRVAARMTSPDLVLFSVEDTGIGIPPEHHENIFREF
                     SQVENPLQDRHRGTGLGLPLCRNLAMLLGGRIWLESEPGHGSTFYAAIPPVYVGESAS
                     LEDAANLPAPEFHRTPILVVEDNPETARLFESYLRSTEFQPILASGAAQAEAWTCRHT
                     PAAVISDICLGEETAWEFLSRLRERLPQLPIIATSVSEESRTAAENAADLFLLKPFER
                     DTLLRELRRLTAQTGTRRLLLVDDNEVSRYIVRELLRQPWLEIDEASNGSQALAAIRD
                     KLPDGIILDLLMPDIGGIDILRQLRANPATEDLPVLIYTSKILNESERAQIESLTARI
                     LRKDDVSTRLSPQPFLDWLTHAGLSPGHSPSGTTV"
     gene            5993..>6555
                     /locus_tag="VHE33_17880"
     CDS             5993..>6555
                     /locus_tag="VHE33_17880"
                     /inference="COORDINATES: protein motif:HMM:NF021534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HVT99378.1"
                     /translation="MRSRRTFLKAALLATAPRWICPASAAAIPETNPVPSSRNDRQAW
                     LAILERVSQPVLEAISRQKLRATMPVEAAPGDEKARAQSTHLEAVGRLLCGLAPWLEA
                     QPGSNLAEEELRTRYREWSRLAIRYGTDPRSPDVLNFGTNHQSLVDAAFLALAIVRAP
                     SELWAKLDTATKQNLVRALKATRKLQPG"
BASE COUNT         1295 a         2444 c         1680 g         1136 t
ORIGIN      
        1 tttccgacta cacttcacct aaaatcagct ttccaaaggg cccctatgca agttcttatc
       61 gtcgaagatc agcccagtga tcttcgtgtc gccacgacgg cggcgcagga atccggattt
      121 gcagacattg aagcgcgatc gtcgatagcc gccgcccgcg atttcctcca gaaggctctg
      181 gacgaaggac ggtctctgcc tgacgccatc gtcctcgacc tcgacctcgg atatgaaagc
      241 ggcttcgaac tgctgcgcta ctggcacagc aatcctcagc tctcgaaggt ttccgtcgtc
      301 gtctggacca tcctcggcaa tcagtatcgc gagatttgcg agatgttcaa ggtcactgcc
      361 tacctcgaca agggtgcggg cggtgccgcc cttcgcaaca cgcttgccgg tttgaagcgg
      421 ctcgcctcct gaacaccggt ccaccgttac gcagtcgcag aacccggcat tttcaggcac
      481 ggacgttgcg cccaactttc acactggcag cgatttatag tacaaggaac ggtgctgttc
      541 gatatcgctg atccccagcg ccaatccatt cccagtccat cctgaagggg gcccctgcat
      601 gaccgatctc gcggagatta tctcccgcaa cgaagaccag atccgctccg aatggattcg
      661 ggacatgggc aagtctgtcc agcgtgcgga cctgatcagc aaaaccgagc tcgaggagca
      721 atgcggcgcc ctgctcaggg cggttgtggc cggcgccaaa agcggcgatg ccacggatat
      781 cgataaccca ggctggagac aggctcgcga atttcttgag agcgtctcgg cttcgcgcgc
      841 tcgccagggt ttctcgccca ctgaggtcgc tacgttcgtc ctctccctca agcagccgct
      901 gttttccgcc attcgccgcg acctgagcaa aaaccccgac aagcttttcg atactgtctg
      961 ggcggccacc gaaatgctcg acgggctcgc gctggtcaca accggcgcct atatcgccac
     1021 tcgcgaagaa ctcatcgtcc gccagcaaca ggagctgatg gagctttcaa cgcccgtggt
     1081 caagctctgg gaaggcgtcc tcgccctgcc catcatcggc actctcgaca gcgcccgcac
     1141 ccaggtcgtt atggagaatc tgctccagac cgtcgtcgcc accaattccc gattcgccat
     1201 catcgacatc accggcgtcc ccaccgtcga caccctcgtc gcccagcatc ttctcaagac
     1261 catcacggct gcccgcctca tgggcgccga gtgcatcatc agcggcgtcc gcccgcagat
     1321 cgcccagacc atcgtccatc tcggcatcaa tctcgaagac gtgatgacca aggccaacct
     1381 cgccgacgct tttgccctcg ccctgcagat gagcggccgc accgtcgtgc gcaggtccac
     1441 cgcctccgcc gagaccaaag gcatcctcgc cgggcccatg acgaagcagg agaactaacg
     1501 tggagcgcat tcccgttctt cgcatgggca gtctgctgct catcaccatc caggtcgaca
     1561 tgcacgacag gctcgccctt cagctccagg acgatgtcac caatcgcatc gccgaatggg
     1621 gctccagagg cgtcctcatc gatatctcgt cgctcgaaat cgtcgactcc ttcatcggcc
     1681 gcatgctcgc caacatcgcg gcgatgtcgc gggtgctcgg tgccgagacc gtcgtcgtcg
     1741 gcatgcagcc cgcggtggcc atcactttgg tcgagctggg tatgtctctg cccggcgtgc
     1801 gaacggctct gagcgtcgaa gccggcatgg agctgctcta cggagcggga gcgggtttcc
     1861 gcacggggag cgatgatgag cgagacgaag cgtgaaactc tcccgctgac gaactccaat
     1921 gacgtggtcc tcgcccggca aaaagtccgg caatgggcca ccgagatgcg tttcactctc
     1981 gtggaccaga ccaagctggt caccgccgcc agcgagctcg cccgcaacac cctcgatcac
     2041 ggtaagggcg gctccatgac catcgagcaa atcgccgccg gcccccgcgt cggcctcagg
     2101 ctcatcttcg aggatcgtgg accaggcatc cccgacatcg aattggcact caaagatggc
     2161 tttaccacgg gtaacggcat gggtcttgga ctcggcggca gcaagcggct ggtcaatgag
     2221 ttctccatcg actccgcccc cgggaagggc acccgcatca cagtagtgcg atggaagtag
     2281 ccttcacaga gtggattccc atcaccgatg agagctcggt gggcgaggcc cgccgccgcg
     2341 ccctgctcgt cgcccagcgg atcggctttg accagacccg ttccggcgaa ctcgcgctgc
     2401 tcgccaccga gtcctcccgc aatgtgctgc tccacggccg gggtggtgaa accgtcatcc
     2461 tcggcatgca ggcgcagggc aaatctcttg cccgcattct cgctatggac aacggcccag
     2521 gcatcgccaa cctcgcccag gccatgaccg atggctactc caccgccggc accatgggcg
     2581 cgggcatggg cgccatgcag cgcatggcca cctccatgga ggtcttcacc ggaaaaaaag
     2641 gaaccgtcct gctgctcgaa ctgggcgagt cttctccccc aggttccctc cgcatcgccg
     2701 gcatggccgt gcctcatccc ggagagcgct tctgcgggga tgcatgggcc tgtcacgaga
     2761 ctccggagcg atccctcatc ctcctcgccg acggcctcgg ccacggacgc gatgccgccg
     2821 aagccgccca ggaagcgact gccgccttcc ataagcgcca ggatcacgcc cccgctgaga
     2881 tcctcggcta cgtacacgac gccctcaaaa aaacccgcgg cgcggttgcc gcgatcgcgg
     2941 aaattcgccc catcgaggcc accctctcct atgccggcgt cggcaacatc tcggccgctg
     3001 tcctcaccgc cgccggatcc cgcagcctcg tctcccacaa tggcaccctc ggcatggtca
     3061 cctcccgcat tcaggagttt cgcattccct ggacccccga tgccttgctg gtcctccatt
     3121 ccgacggcct gcagtcgaag tgggacctct ctgcctaccc cggcctcatt acccgccacc
     3181 ccgccgtcat cggcggaacc ctcgtgcgcg acttccgccg ccagcgcgac gatgccagtg
     3241 tggtcgtggt aaaagctgca tagcatggcg attccggtct tccatctgcc tctccgcacc
     3301 gaacgcgatg tcgtgcaggc tcgccagcgc gcccgcgaag tcgccgcgga gctcggcctc
     3361 gacaatcagg accagatccg catggccact gccgcctcgg agattgctcg caatgccttc
     3421 cgctacgccc gcaatggcgc cgtggaattt gccgtcggac tggaagatcc ccagcatctg
     3481 gaaatcaccg tctccgacac cggtcccggc atcgccaatc tcgacgagat cctcgacggc
     3541 cgctacaaat ccaccaccgg catgggcatc ggcctgctcg gcacccgccg cctcatggac
     3601 cgcttcgaga tcgacagcca gcccgccggt accaccgtcc gcatggtgaa gcacctcccg
     3661 cagcagcatg cgtccatgac ccagcgggcc gttcgcgagc tgaacgcgaa actgaagcag
     3721 cgcactcccg agagccctta cgaagaaatc gagcagcaga accaggaact actcaagacc
     3781 cttcaggaac tgcgctcccg ccaggaagaa ctccagctgc tcaatcgcga actggaagac
     3841 accaatcgcg gcgtcgttgc cctttacgcc gagctcgacg agcgcgccga ctatcttcgc
     3901 cgcgcgtccg aactcaaaac taaattcctc tccaacgtct cgcacgaatt ccgcaccccg
     3961 ctcaactcca tcatttccct cgctcgcctc ctcatggatc gcatcgacgg cgatctgaac
     4021 tccgaacaga ccaagcaggt ccgcttcatc gaaagctccg cacgcgacct ccaggagatg
     4081 gtcaacgacc tgctcgatct cgccaaggtc gaagccggca aaattcgtat ccgccccaaa
     4141 cacttccagg tgcacgagct gttcagcgcc ctcaagggta tgcttaagcc cctgctcgcc
     4201 gacaacacct ccgtcgacct catctttgag gatgcgaccg aattgcccgc cctccacacc
     4261 gatgagggca aagtctcgca aatcctcagg aaccttatct ccaacgcgct caagttcacg
     4321 cccgcaggcc acgtgcgcgt tgctgcccgg atgacctcgc cggaccttgt gctcttttcg
     4381 gtcgaagaca ccgggatcgg cattcccccc gagcatcacg aaaatatctt tcgcgagttc
     4441 agccaggttg agaaccctct ccaggaccgc caccgcggca ccggtctcgg cctccccctc
     4501 tgtcgcaatc tcgccatgct cctcggcgga cgcatctggc tcgaaagtga acccggccac
     4561 ggttccacct tctacgcagc cattccgccc gtctacgtcg gcgaatcggc ctcgctcgag
     4621 gacgcggcca acctgcccgc tcccgaattc caccgcactc ccattctggt cgtcgaagac
     4681 aatcccgaga ccgctcgcct cttcgagagc tacctccgca gtacggaatt tcagcccatc
     4741 ctggcctcag gcgctgccca ggccgaagca tggacctgcc gccacacgcc cgccgcggtg
     4801 atctcggata tctgtctcgg tgaagagact gcctgggaat tcctgagccg cctgcgcgag
     4861 cggctgccgc agctgcctat catcgccacc agcgtttcgg aggaatcccg caccgctgca
     4921 gaaaatgcgg ccgacctttt cctcctcaag cctttcgagc gcgacactct cctgcgcgaa
     4981 ctgcgccgtc tgaccgcaca gaccggcacc cgccgcttgc tgctcgtcga cgataacgaa
     5041 gtctcccgct atatcgtccg cgagcttctc cgccagccct ggcttgagat cgacgaggcc
     5101 agcaacggct cccaggcact cgccgccatc cgcgacaagc ttcccgacgg aatcatcctc
     5161 gatctcctca tgcccgacat cggtgggatc gacattctcc ggcagttacg agcaaatccg
     5221 gcgacagaag atctccccgt gctgatttac acctcgaaga tcctgaacga atcggagcga
     5281 gcccagatcg agtcgctgac cgcccgtatt cttcgcaagg atgacgtctc cacccgcctt
     5341 tcgccgcaac ccttcctgga ctggctgacc cacgcggggc tctcccccgg ccattcccca
     5401 tccggcacaa cggtctgaac gtcatctccg cagtacagcc cactccgcag tacggccgaa
     5461 cgaatcagcg aagccgaagc ccggtgcttg tcgccaggtt cgcgaaccca tcgcgcgcgg
     5521 ccaggttcac cccacagcaa gcagaagacg ccttcccgac agacatttca acagccgcaa
     5581 tccaacggcc cgcccaaaag taccgtcgat ccgcccattt tgaaacttca ttccccccgc
     5641 caggttcgcg aacccatcgc ccgcggccag gttcacccca cagcaagcag aagacgcctt
     5701 ccagacagac ttttcaacac ccgcaatcca acggcccgcc caaaggtacc gtcactctgc
     5761 ccattttgaa acttcattcc cccaccaggt tcgcgaatcc atcgcccgcg gccaggttca
     5821 ccccacagca agcagaagac gccttcccga cagccatttc aacagccgca atccaacgat
     5881 ccgcccaaag gtacccctca ctgaccattt cggaactcag ccgccaacca tccccatccc
     5941 ccgcgattac aatacccacc ggcattcaaa ccaccgccta agaggaaatg cattgcgatc
     6001 cagacgaacc ttcctgaaag ccgccctcct cgcaactgca ccgcgatgga tctgcccggc
     6061 gtcagcggct gcgatccccg aaaccaaccc cgtccccagc tcccgcaacg accgccaagc
     6121 ctggctcgcc atcctcgaac gtgtctccca gcccgtcctc gaggccatca gccggcaaaa
     6181 actgcgcgcc accatgcccg tcgaagccgc ccctggcgac gaaaaagccc gcgcccagtc
     6241 cacccatctc gaagccgtcg gccgcctcct ctgcggactc gctccctggc tcgaagccca
     6301 gcccggcagc aacctggccg aagaggaact ccgcacccgc tatcgcgaat ggtctcgcct
     6361 cgccatccgc tacggcaccg atccccggtc gcccgacgtc ctcaacttcg gcaccaacca
     6421 tcagtccctc gtcgatgccg ccttcctcgc cctcgccatc gtccgcgccc ccagcgaact
     6481 ctgggccaaa ctcgacacag caacaaagca aaacctcgtc agggccctca aagcaacgcg
     6541 caaactccag cccgg
//
LOCUS       DASZYN010000690         1760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_618108, whole genome shotgun sequence.
ACCESSION   DASZYN010000690 DASZYN010000000
VERSION     DASZYN010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618108"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..1096)
                     /locus_tag="VHE33_17885"
     CDS             complement(197..1096)
                     /locus_tag="VHE33_17885"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HVT99379.1"
                     /translation="MEVHLIDGTYELFRHFYAVPRARDSEGREVGAVRGVLASLLGML
                     KGGVTHIGVATDHVIESFRNSLWAGYKTGEGIDPDLLSQFPLLEEVLTAAGMVVWPMV
                     EFEADDALASAASAAAQNPNVERVVICTPDKDLAQCVRGKRVVQLNRRTGAIYDESAV
                     RQKFGVPPESIPDYLGLVGDAADGYPGLPGWGAKSTAAVLAKFRHLESIPHDSREWQV
                     NAGRADTLAATLVRNFEDALLFRTLATLRTEIVLFDDVDRVRWMGPTPAFDAIGKRLD
                     GAITEKTPRKDRPVPPRRTMDKP"
     gene            1217..1759
                     /locus_tag="VHE33_17890"
     CDS             1217..1759
                     /locus_tag="VHE33_17890"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HVT99380.1"
                     /translation="MKRFLLCASLLALTSTLFAQQKPPASPAETATATIGGHTVTISY
                     NSPGVKGREGQIFNQGGLIQKTHKEYPVWRAGANKATALHTDADLTIGTLDVPKGDYT
                     LFVNIADPDNWVLIVSKKTGEWGLAYDPSEDLGKTPMTMSKPSSMVENLKWTITGDGN
                     KGTITLAWENHSASVSVTAH"
BASE COUNT          369 a          606 c          457 g          328 t
ORIGIN      
        1 cactgcgtag cgaagatcct gcagccagac gttcatcgac cctcccgcgc tgataacgag
       61 gatcatagga cacggagaca ctctctggct gccgtgaaac aggtttgtga cggcttggct
      121 acggctctcc ggggtgcccc accctggtta cttccactgt tgacgcggtc agcaaccacc
      181 gcgtctccgc cgagacttat ggtttgtcca tggttcttcg cgggggtacc ggtcgatcct
      241 tccgcggcgt cttttccgtg atcgctccgt ccagccgctt tccgatcgca tcgaacgcgg
      301 gcgtgggtcc catccaccgc acccggtcga catcgtcaaa caagacgatc tccgtccgaa
      361 gcgtagccag cgtccgaaac aatagagcat cctcaaagtt gcgcaccagg gtcgccgcga
      421 gagtgtccgc acggccggcg ttcacctgcc actcccggct gtcatgagga atggactcga
      481 gatggcgaaa tttagccagc accgccgccg tcgattttgc gccccatccc ggcagccccg
      541 gataaccatc cgccgcgtcg cccaccagcc ccagatagtc aggaatcgat tcgggcggaa
      601 ctccaaattt ctgccggacg gcactctcgt cgtagattgc gcccgttcta cggttcagtt
      661 ggacaacgcg cttgcccctg acgcactggg ccagatcctt gtccggagta cagatcacca
      721 cccgttccac atttggattc tgcgcagctg cggatgcggc agacgccaga gcgtcgtccg
      781 cctcaaactc caccatcggc caaaccacca ttccggccgc ggtgagcact tcctccagca
      841 gtggaaactg agaaagaaga tcgggatcga tgccttctcc ggttttgtat cccgcccaca
      901 gggagtttcg aaacgactcg atcacatgat ccgttgccac tccgatgtgt gtgaccccgc
      961 ccttcagcat gcccaataaa gaggcgagca caccccgaac cgcacccacc tcgcgccctt
     1021 cggagtcgcg tgcacgcggc actgcgtaaa agtggcgaaa cagctcgtag gtgccgtcga
     1081 tcagatggac ttccacgctt tgatccaatg gagactaccg gccacaggtt accagccaca
     1141 cgctcgctcc cctacaatgc ctccgtgggt ccgggcccct ttctcccggc tcgaacccca
     1201 acaaggagaa tcatcgatga agcgtttcct gctgtgtgcc agtcttcttg ccctcacctc
     1261 caccctcttc gctcagcaga agcctcctgc cagccccgct gaaaccgcca ccgctacgat
     1321 cggaggccat accgtcacca tcagctataa ctcgccaggc gtgaaaggcc gcgaaggcca
     1381 gatcttcaat cagggcggcc tcatccagaa aacgcacaag gagtaccccg tctggcgcgc
     1441 cggtgccaac aaagccactg ccctgcacac cgacgctgac ctcaccatcg gcaccctcga
     1501 tgtccccaaa ggggactaca ctctgttcgt taacatcgcc gaccccgata actgggttct
     1561 gatcgtgagc aagaagaccg gcgaatgggg cttggcctac gatccctccg aggatttggg
     1621 caagacgccg atgaccatga gcaagccttc atcgatggtc gagaacctga agtggacgat
     1681 cacaggcgac ggcaacaaag gcacgatcac gttggcctgg gaaaaccaca gcgcctccgt
     1741 ctctgtaacc gctcactgac
//
LOCUS       DASZYN010000691         8398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1133445, whole genome shotgun sequence.
ACCESSION   DASZYN010000691 DASZYN010000000
VERSION     DASZYN010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8398
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133445"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..1224)
                     /locus_tag="VHE33_17895"
     CDS             complement(217..1224)
                     /locus_tag="VHE33_17895"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HVT99381.1"
                     /translation="MNHDLENVRRLAAKLRENLAKCAEDPGVEPVHDTRTGTRRLQAT
                     LEDLVRQSPAGEAGEPMRAAATATMKTLKKIRRAAAPVRDLDVHRRLLEKLVRRAVEP
                     RDNKDQKAFREPAGNNTPNLLETAEAGLRTTALEQQADDLDAWLKHRRDEQAGGLKAQ
                     TNDLITRFDKRLGELEAALHAQRARRARKKSPGVVALDAFARLASEMQVLDATNLHDF
                     RKGAKKARYVAELAAQGDAQAKQVGETLKKLQDEIGDWHDWLVLAEEAHAALGERATD
                     LTALIEGEREQHFVAAMNTATKLRGRLMGEWLAVSRRPVTRAASPARKRPAGTLHRAA
                     S"
     gene            1287..3311
                     /locus_tag="VHE33_17900"
     CDS             1287..3311
                     /locus_tag="VHE33_17900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HVT99382.1"
                     /translation="MTYPPLSKSQSAAPSAEPPLYATPSRAWTLLVLVFIAVYFAALF
                     TPALLDDADATHAQAAQHIATSGDWVTLYVNGIRYLEKPPLPYWIVAVDYHLFGFNVF
                     ATHLPMALAVLGCAWIAWTWGRRAWGDRAAFYAALAMLTCVGVFLFTRTLIPESLLTF
                     FLLLSLYSFLTGVEDRKPWRLYVAYGSLALALLAKGLIAPVFFVAAVVPYLIITGDWK
                     KWRQFHLLTGLLLFLAIGAPWHILAGLANPDHGNPIGNVPTPGHVHGFFYFYFINEHF
                     LRFLGLRFPHDYNRQPFYVYWLGQLIWLFPWSLFLPVVIRRAWRNRHLVYSDLRYDAT
                     NTIQFLDPKLTAYESSALAAHLRFRARTSLLLALYAGFILIFFALSTNQEYYTWPMYP
                     AVLMLIAGGLSVIEESQSKVRKGSSGWLTGVHIFFLAAGLIAAALLAWGLWQSRHLPY
                     IADIGTLMAHRGVGGYTLSMSHFFDLTGPSFAALRLPAALAAIAFFVGPLAAWYLRRK
                     GHAFEATSSVAFTIAVILIAAHIALVRFQPMLSSQAIAHTINRITAAPADADAQLMLY
                     GDQADGSSILFYTQRHALLVHGATNYFAPDPETHGEVFGSSMIWGSDYPDAPHIFLSD
                     QDLLRMWGSGQRKLLYVPGDFRNHVEALLGSKLHQIQEVSDKTLFTDRPL"
     gene            complement(3438..4334)
                     /locus_tag="VHE33_17905"
     CDS             complement(3438..4334)
                     /locus_tag="VHE33_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99383.1"
                     /translation="MMATRPMRGRLAVALMLALPLLNGCLWHTRRVPQARMPANVQSA
                     PPAQLVDSINKTYDAISTMSATVTFTATEGGTLKGKETTYTSFSGYILMRKPEALRVI
                     GFLPVVHTRAFDMASDGKDFKLWIPPKNKVIEGTNKITQPSKNALENLRPFIFSDSLL
                     IRKIGPDDLLLVTTDSPTVMNPNTKKLEFQPEYLLTVLNREGGGNVLQAKRVVHFSRI
                     DLRPIQEDIYGPDGQVQTQAIYGPQQTFGTEHYPGTITIKWPLQEQQILITFQKVILN
                     QPLNDEQFQLTIPEATPVQKLP"
     gene            complement(4331..5329)
                     /gene="mltG"
                     /locus_tag="VHE33_17910"
     CDS             complement(4331..5329)
                     /gene="mltG"
                     /locus_tag="VHE33_17910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HVT99384.1"
                     /translation="MRRFFLLLFFLALVGAGVVAWTLLMPAGPQTETFVEILPGTPTS
                     RIASQLKDDGIIRSRYVFLALREWKGGTLKAGEYRFDHPVPMKEVLDRIHRGDVYTIA
                     LTIPEGSNIFDIGARIEADGLGSKEAFLTAAKQDVALVHDLDGNAASVEGYLFPDTYR
                     FARHTEPQEMIAVMVKRFRQATQGIGLTSDYHPIVTLASLVERETPIDSERPLVASVF
                     ENRLDKNIPLMTDPSVIYAALLDGRYRGTIYASDLQANSPYNTYLHAGLPPGPICNPG
                     LKSLQAALHPAQTDYLYFVAAGADPSGQSRFSTTLEQHAKDVEAYRRAVRNAGSSQ"
     gene            5426..5869
                     /gene="ruvX"
                     /locus_tag="VHE33_17915"
     CDS             5426..5869
                     /gene="ruvX"
                     /locus_tag="VHE33_17915"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HVT99385.1"
                     /translation="MQPRLLGLDFGMRRIGVAVSDELGLTAQPVLTLVRKNAKQDLKS
                     LGRLIRKFGCQAVVLGHPLHMSGDVSPLALKTQAFADTLREAFSLPVHLWDERLSSAE
                     AHRHLEAAGRPAADHRAVVDQVAAVLILQSFLDAHRSGAASYNEG"
     gene            6012..6494
                     /gene="greA"
                     /locus_tag="VHE33_17920"
     CDS             6012..6494
                     /gene="greA"
                     /locus_tag="VHE33_17920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HVT99386.1"
                     /translation="MTEQIKKKLLEEIKELEHELTHELPAEIKKAVAMGDLSENAEYH
                     MAKQRQDFVNARLGQLKKRMAELALVNLANIPRDRAAFGSTVLVYDTSKDEKIEYKLV
                     TSEESDVSKGLISTTSPIGKALVGKQVGDMTTVVTPNGKRELEVLKLATIHDGAEAAS
                     "
     gene            6538..7293
                     /locus_tag="VHE33_17925"
     CDS             6538..7293
                     /locus_tag="VHE33_17925"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HVT99387.1"
                     /translation="MEENKPITWTRAFGAGCGVILQAIVNGLALTRISPNVLTFLGLV
                     INTAAAILFGFGNQHNYVRMFLYAALVIIGAGIFDMVDGRVARQTQQVTVFGAFFDSV
                     IDRYSDVVLFFGLLVFYARGNRLFYVFLAAFVMVTSLMVSYTRARAEALIGKCKVGFM
                     ERPERVVLIIIGALFERWDAMAPVLWILAVISTITVVHRILYTYQKTRHLAPVTEVHH
                     IVQPSVPVASVQPITMREADARPELRASNAPRA"
     gene            7405..>8398
                     /locus_tag="VHE33_17930"
     CDS             7405..>8398
                     /locus_tag="VHE33_17930"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HVT99388.1"
                     /translation="MKIVRVLAVTALLTTGLLAVPHASAQTFPVLAIGSLNQSRAGAF
                     QDLSGLTSPLENGVRGNELGGLGSALTWASGDTFLALPDRGPNAVPYDSNIDDTVTYI
                     NRFQTIQMRLDPNKPGSGLPFTLTPHLKETTLLWSLTPLFYGAGDGQVGSGAPSQNRF
                     FVHYFTGRSDGFDPNHDSGDAYDARFDTEGIRVSNDGRSVFISDEYGPYIYQFDRATG
                     LRLRSFRLPDSFYVDNLSPMGATEISGNTSGRTANKGMEGIAITPDGRTLVGIMQNAL
                     IQDASEGGAAKNLLRIAVIDIASGRMTHEYGYLLTSGSGVSEILALNNHEFLVDE"
BASE COUNT         1468 a         2854 c         2325 g         1751 t
ORIGIN      
        1 ggcgatacga ttcctgtatc gaagacgctc gcgcccagtc gcgtgacctc gcgatcctca
       61 tgcacgatgc gcaaacgatg ctgcaccacg cggccgatct tcagcctgca ggagttcgat
      121 ccgatgtcga tggctgcgaa ggtatgcatg atggggcgat tagcagcgaa cttcagcgcc
      181 ttagggattc attcagcaca atacacgagt tcgcgctcac gaagccgcgc gatgcagcgt
      241 tcctgccggc cgctttctgg ccggactcgc cgctcgcgtc accggacgcc gcgaaaccgc
      301 cagccactcg cccatgagcc gcccgcgcaa cttcgtagcc gtgttcatcg ccgccacgaa
      361 atgctgctcg cgctcccctt caatcagcgc ggtcagatcc gtcgcgcgtt cgccgagagc
      421 cgcgtgagcc tcctcggcga gcacgagcca atcgtgccag tcgccgatct cgtcctgcag
      481 cttcttcagc gtctcgccca cctgtttggc ctgcgcatcg ccctgagccg cgagttcggc
      541 cacatagcgt gccttcttgg cgccttttcg aaaatcatgc agattcgtgg cgtcgagcac
      601 ctgcatctct gaggcgaggc gcgcaaaggc gtccagcgcg accacgcccg gcgacttctt
      661 tctcgcacgc cgcgctcgct gtgcatggag cgcagcctcc agttcgccaa ggcgtttgtc
      721 gaacctggtt atcagatcat tggtttgcgc cttcagcccg ccggcctgtt cgtcgcgccg
      781 atgcttcagc caggcatcca gatcatcggc ctgctgctcc agagcggtcg tccgtagtcc
      841 tgcctccgcc gtctccagaa ggttcggggt attgttcccc gcaggctccc tgaacgcttt
      901 ttggtctttg ttgtcgcgcg gctcgaccgc ccgcctcacc agcttctcca gcagcctgcg
      961 atgcacatcc agatctcgca ccggagccgc ggcacggcga atcttcttca gcgtcttcat
     1021 cgttgctgtg gccgcggccc gcatgggctc gccggcctcg cccgccgggc tctgccgaac
     1081 caggtcttcg agcgtggcct gcaggcgccg cgttccggtg cgcgtgtcat ggaccggttc
     1141 cacccccggg tcttctgcac actttgcgag attttcgcgc agcttcgccg ccagccgccg
     1201 cacattctca aggtcgtgat tcatggccgc gcccgtatcc ccaggccctc actgatattc
     1261 tgaactctct gccaggcttc gaccaaatga cttatcctcc actgtcaaaa tcccagtcgg
     1321 cagctccgtc ggcggagcct ccgctctacg ccacgccttc gcgcgcctgg actctgctgg
     1381 ttcttgtctt tattgctgtc tatttcgcag ccctcttcac cccggcgctc ctggacgacg
     1441 ccgacgccac ccacgcgcag gcggcgcagc acatcgccac cagcggcgac tgggtcacgc
     1501 tctacgtcaa cggtatccgc tatctggaaa aaccgccgct gccctactgg atcgtcgcgg
     1561 tggactatca cctcttcggc ttcaacgtgt ttgccaccca tcttcccatg gcactggccg
     1621 tcctgggctg cgcctggatc gcctggacgt ggggccgacg cgcatggggc gatcgcgccg
     1681 ccttctacgc cgcgctcgcg atgctcacgt gcgtgggggt ctttctcttc acccgcacgc
     1741 tgattccgga atcactgctc acgtttttcc tgttgctgtc gctgtacagc tttctcaccg
     1801 gagtcgaaga ccgcaagccc tggcgtctct acgtcgccta tgggtcgctc gccctggcgc
     1861 tgctcgccaa agggctgatc gcacctgtct tttttgtcgc ggcggtcgtg ccttatctca
     1921 tcatcaccgg cgactggaag aaatggcggc agttccatct gctgacggga ttgcttctgt
     1981 ttcttgctat tggcgcgccg tggcacattc tcgccggact ggccaatccc gatcacggca
     2041 acccaatcgg caatgtgcca acgcccggtc acgttcacgg tttcttttac ttctacttca
     2101 tcaacgagca cttcctccga ttccttgggc tccgcttccc gcacgactac aaccgccagc
     2161 cgttctacgt ctactggctc ggccagttga tctggctgtt tccgtggagt ctcttcctgc
     2221 ccgttgtcat tcgccgcgcc tggcgcaatc gccacctcgt gtattccgat ctgcgttacg
     2281 acgccacgaa tacgattcag tttctcgatc caaaactgac cgcgtacgaa tcctccgctt
     2341 tggccgcgca tctccgcttc cgcgcccgca ccagcctgct gctggcgctc tatgcgggct
     2401 tcatcctgat cttcttcgcc ctttccacca atcaggagta ctacacctgg ccgatgtatc
     2461 ccgccgtgct catgctcatc gccggcggcc tctcggtcat cgaagagtcg cagagcaaag
     2521 tgcgcaaagg ctcctccggg tggctgaccg gcgtacacat cttcttcctc gcggcggggc
     2581 tgatcgccgc ggctctgctg gcctggggac tgtggcagtc gcgccacctt ccctacatcg
     2641 ccgacatcgg cacgctgatg gcgcatcgcg gcgtcggcgg ctacacgctg tcgatgtccc
     2701 acttcttcga cctgaccggg ccgtcgttcg cggcattgcg cctgcccgcc gctctcgcag
     2761 cgatcgcttt ctttgttggc cctctggccg cctggtatct ccgccgaaaa ggtcacgcat
     2821 ttgaggccac ctcaagcgtg gccttcacca tcgccgtcat cctcatcgcg gctcacattg
     2881 cgttggtgcg cttccagccc atgctctcca gccaggcgat tgctcacacc atcaatcgca
     2941 tcacggcggc tccggccgat gccgacgctc aactgatgct ctacggcgac caggccgacg
     3001 gctcgtcaat cctcttttac acgcagcgtc atgccctgct cgtgcacgga gccaccaact
     3061 acttcgctcc cgatccggag acgcacggcg aagtcttcgg ctcctccatg atctggggtt
     3121 cggattatcc cgacgcgccg cacatcttcc tcagcgacca ggacctgctg cggatgtggg
     3181 gaagcggcca acgcaagctg ctttacgttc cgggcgactt ccgcaaccat gtcgaggccc
     3241 tgctgggttc gaagctacac cagatccagg aagtctcaga caagacgctt ttcaccgacc
     3301 gcccactctg agatggcgtg cggtaatccg cgctcagcgt tcggcaaggc gccagcctga
     3361 tcatgttccg ggcatctggt ggtctcgtgc gagttacggc cgaacgcgga aagcggaacg
     3421 ccgaacgccg aacgccacta aggcagcttc tgcaccggcg tggcttccgg aatcgtcaac
     3481 tggaactgct catcgttcag aggctgattc aggatcactt tttggaacgt gatcaggatc
     3541 tgctgctcct gcagtggcca cttgatggtg atggttccgg gataatgctc agtcccaaaa
     3601 gtctgctgcg gaccgtagat ggcctgcgtc tgcacctgcc cgtccggtcc gtaaatatct
     3661 tcctggatcg gacgcagatc gatgcggctg aaatgaacca cacgtttggc ctgcagcaca
     3721 ttgccgccgc cttcccgatt cagcacggtc agcagatact ccggctggaa ctccagcttt
     3781 ttcgtgttcg gattcatgac cgtcgggctg tcggtcgtca ccagcagcag atcgtccggc
     3841 ccgattttcc ggatcagcag cgaatcggaa aaaatgaatg gccgcagatt ctccagagca
     3901 ttcttcgagg gctgggtgat cttgttcgtt ccctcgatca ccttattctt cggcgggatc
     3961 cataacttga aatccttgcc gtcgctcgcc atgtcgaagg cacgcgtatg gaccacgggc
     4021 agaaatccga tgacccgcag cgcctccggc ttccgcatca ggatgtaccc gctgaacgag
     4081 gtgtaggtgg tttccttgcc tttcagcgtg ccgccctcgg tcgcggtgaa ggtcaccgtg
     4141 gcgctcatgg tgctgatcgc gtcgtaggtc ttgttgatcg aatccacgag ttgtgccggc
     4201 ggcgcggact gcacgttggc cggcatcctg gcctgcggaa ccctgcgcgt gtgccagagg
     4261 cagccattca gcaggggcag cgcgagcatc agcgccaccg ccagccggcc tcgcatcggg
     4321 cgcgtcgcca tcattgcgac gaacccgcat tgcggactgc gcgacggtag gcttccacat
     4381 ccttcgcgtg ttgctccagg gtcgtggaaa agcgcgactg gcccgaagga tccgctcccg
     4441 cggccacaaa gtagagatag tcggtctgcg ccgggtgaag ggctgcctgc agcgacttca
     4501 ggcctggatt gcagatgggt ccgggcggca gacctgcatg gagataggtg ttgtacggcg
     4561 agttggcctg caggtccgac gcgtaaatcg ttccccggta ccgcccatcc agcagcgccg
     4621 cgtagatcac cgaggggtcg gtcatcaggg gaatgttctt gtcgaggcgg ttctcgaaaa
     4681 cgctggccac cagcggccgc tcggaatcga tcggcgtttc cctttccacc agggaagcga
     4741 gggtaacgat cgggtggtag tcactggtaa gcccgattcc ctgcgttgcc tgacgaaaac
     4801 gcttcaccat caccgcgatc atctcctgcg gttcggtatg gcgcgcgaag cgatacgtgt
     4861 ccgggaacag atacccctcc acgctggcgg cattcccatc cagatcgtga accagcgcaa
     4921 cgtcctgctt cgcggcagtc agaaaagctt ccttcgatcc cagcccgtcc gcttcgatcc
     4981 tggccccgat gtcgaagatg ttcgagccct ccgggatcgt cagcgcaatc gtatagacgt
     5041 cgcctcgatg gatgcggtcc agcacttctt tcatgggtac cgggtgatcg aaacgatatt
     5101 cccctgcctt cagcgtgccg cccttccatt cgcgcagcgc cagaaagacg tagcggcttc
     5161 ggatgattcc atcgtctttc agctggctcg cgatgcggct ggtcggcgtc ccgggcagaa
     5221 tctccacaaa ggtttcagtt tgcggaccag cgggcatcag cagcgtccag gcaacaacgc
     5281 ccgcgcccac caaagccagg aagaaaagaa gaagaaagaa gcgtcgcacc ctggagaaaa
     5341 cctgttcctg gaataacttc cttttgattc cctaacccat tctaagatgc ggctttcgct
     5401 tcgtgctcac ggtactatcg gtggtgtgca accccgcctg ctcggcctcg atttcggcat
     5461 gcgccgcatc ggcgttgccg tctcggacga actcggctta acggcacagc ccgtcctcac
     5521 gctggtccgc aaaaatgcga agcaggacct gaaatccctg ggccggctga tccgcaaatt
     5581 tggctgtcag gcggtcgtgc tcggccatcc cctccacatg tcgggcgacg tcagtccgct
     5641 ggcgctgaaa acccaggcgt tcgcagacac cctgcgcgag gctttctccc tccccgttca
     5701 cctgtgggat gagcgcctca gctcggccga agcgcaccgt cacctggagg ccgccggacg
     5761 ccccgcggca gaccatcgtg cggtcgtgga tcaggtggct gccgtgttga ttttgcagag
     5821 tttcctcgat gcgcaccgct ccggggcggc atcgtataat gagggttgag gcggaacccc
     5881 agcctccgga ctcatactcc ttttccaggg tcttgatccc ttatcgcggc gggaaggaaa
     5941 gctggttgcc ttcaccgcac agcgatggct gttgcctgag ggcgaccggg accccagggg
     6001 aatccaaatt catgaccgaa cagatcaaaa agaagcttct ggaagaaatc aaagagcttg
     6061 aacacgagct gacgcacgaa ctacctgccg aaatcaaaaa ggcggtggct atgggcgacc
     6121 tgagcgaaaa cgccgaatac cacatggcca agcagcgcca ggacttcgtg aacgcgcgcc
     6181 tggggcaact gaagaagcgg atggccgagc tggctctggt caacctcgcc aacattccgc
     6241 gcgatcgtgc cgcctttggt tccacggttc tggtttacga cacctcgaaa gatgagaaaa
     6301 tcgaatacaa gctggtcacc agcgaagaat cggacgtgag caagggcctc atctccacca
     6361 cctctcccat cggcaaggcg ctcgtgggca aacaggtggg cgatatgacg accgtggtca
     6421 cgcccaatgg caagcgggaa cttgaggtcc tgaagctggc cacgatccat gacggtgcgg
     6481 aagccgcctc gtagcctgac cctgaacgct tcgaacggaa gcagaagccg ggacgcagtg
     6541 gaagaaaaca aacccatcac ctggactcgc gctttcggcg ccggatgcgg cgtcattctg
     6601 caggcgatcg tcaacgggct cgccctcacc cgcatttcac ccaacgttct caccttcctc
     6661 ggcctggtca tcaataccgc cgccgccatc ctctttggct tcggcaacca gcacaactac
     6721 gtccgcatgt tcctgtatgc cgccctcgtc atcatcggcg ccggcatctt cgacatggtc
     6781 gacggccgcg tcgctcgcca gacccagcag gtcaccgtct tcggcgcttt cttcgactcg
     6841 gttatcgacc gctactccga cgtcgtcctc ttcttcggac tgctggtctt ctacgcacgc
     6901 ggcaatcgcc tcttctatgt ctttctcgcc gcgttcgtga tggtcacctc gctgatggtc
     6961 agctacacgc gcgcgcgtgc cgaagcgctg atcggcaagt gcaaggtcgg gttcatggag
     7021 cggcccgagc gcgtcgtgct catcatcatc ggcgccctgt tcgagcgctg ggacgcgatg
     7081 gcccccgtcc tctggatcct cgccgtcatc tccaccatca cggtcgtcca ccgcattctc
     7141 tacacctacc agaagacccg gcacctggct cctgtcaccg aggttcacca tatcgtgcaa
     7201 ccgtccgttc cggttgcgtc ggtgcagccg atcacgatgc gtgaggcgga tgcccgcccg
     7261 gaactacgcg cgtcgaacgc gccgcgcgcc tagctcaaca ttcctgaaaa atttcatcga
     7321 attttgaccg cgcgttcttc atgcgcaggt ctcgcgccct cctcatcagt gtttagaatc
     7381 gctcctccgg aggagcttct cactgtgaag attgttcgcg ttctggctgt caccgcactt
     7441 ctgacgaccg gcctgctcgc cgttccgcac gcctcagctc aaacctttcc cgttctcgcc
     7501 atcggttccc tcaaccagtc tcgcgccggc gcattccagg acctctccgg cctcacctct
     7561 ccgctcgaaa acggagtccg cggcaacgaa ctgggcggcc tcggttcggc gctcacctgg
     7621 gcttccggcg acacctttct cgccctgccc gatcgtgggc ccaacgcagt cccctacgac
     7681 tccaacatcg atgacacggt gacgtacatc aaccgcttcc agaccatcca gatgcgtttg
     7741 gacccgaaca agccgggaag cggtctgccc ttcacgctta caccccacct gaaagaaacc
     7801 acgctgctct ggagtctcac cccgctgttc tacggagccg gcgacgggca ggtgggctcc
     7861 ggcgctccgt cccagaaccg tttcttcgtc cactacttca ccggccgctc cgatgggttc
     7921 gatcccaatc atgactccgg cgacgcctac gacgcccgct tcgacacgga aggcattcgc
     7981 gtttcgaacg acggacgcag cgtcttcatc tccgatgaat acggtcccta catctaccag
     8041 ttcgatcgcg cgacgggtct gcgcctgcgc tccttccgcc tgcccgattc gttctacgtc
     8101 gacaatctca gcccgatggg tgccaccgaa atctccggca acaccagtgg ccgcacagcg
     8161 aataagggca tggaaggcat cgccatcacg cccgacgggc gtaccctggt cggcatcatg
     8221 cagaatgcgc tgatccagga tgccagcgag ggtggcgccg ccaaaaacct gctccgcatc
     8281 gccgtcatcg acatcgcatc gggccgtatg acacacgagt acggatacct gcttacatcg
     8341 ggcagcggcg tcagcgaaat tctggcgctc aacaatcacg aattcctggt cgacgagc
//
LOCUS       DASZYN010000692         1671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_234320, whole genome shotgun sequence.
ACCESSION   DASZYN010000692 DASZYN010000000
VERSION     DASZYN010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234320"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VHE33_17935"
     CDS             complement(<1..939)
                     /locus_tag="VHE33_17935"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HVT99389.1"
                     /translation="MRNLVAVLVFAALVALAHGQDTSAAPFRNPRLPIEERITDLIHR
                     LTLEEKAQQLNHTNHGLPRLGIPMWGGWNQTLHGVWSKEPTTLFPAPIAMGATWDPDL
                     VHSVAAAMSDEARALYNAHAEGPRGPMGLVYRSPVINLVRNPRWGRIQEVFSEDPYLT
                     GRMAVAYVRGLQGDDPSHLKLAATVKHFAVYNVETGRQHLSADVDERNLMEYWLPQWR
                     AAIEEGHAQSVMSSYNAINGTPDAVNRYLLTDILRGQWGFDGFVTDDLGAVALLTSGS
                     ERNPTEPGQRLTEDPVVAAAMAIKAGNDSDDPEFEKN"
     gene            complement(1023..>1671)
                     /locus_tag="VHE33_17940"
     CDS             complement(1023..>1671)
                     /locus_tag="VHE33_17940"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT99390.1"
                     /translation="GALAAMQPDRVLVECSTLSPAWIAELHGAARDRGLRMVEAPVTG
                     SRPQAEAGQLTFIVGADDADLAAVEPVLRVMSKEVVHLGQVGSGTQLKLINNFLCGVQ
                     VTSFAEALAWIERSGLKRESAFEFLKKGAPGSGILTAMADRMTRRTYEVNFLLRLMAK
                     DLRYAREAAAREGVPLSTSAPAEELFRKAQEEGLGEKDMSAVAETVRAGAHKPVK"
BASE COUNT          303 a          504 c          528 g          336 t
ORIGIN      
        1 gttcttctcg aactcgggat cgtcggagtc attgcccgct ttgatcgcca tggcggcggc
       61 gactaccggg tcctcggtca agcgctgtcc gggttcagtg ggatttcttt ccgaaccgct
      121 ggtgagaagt gccaccgcgc cgagatcgtc ggtcacaaac ccgtcgaatc cccattgccc
      181 gcgcagaatg tcggtgagca ggtagcgatt cacggcatcg ggcgttccgt tgatggcgtt
      241 gtagctggac atcactgact gcgcatggcc ttcctcgata gcggctcgcc attgcggcag
      301 ccagtactcc atcagattgc gctcatcgac atcggcggaa aggtgctgcc gcccggtctc
      361 gacgttatag accgcaaaat gtttgacggt cgcagcgagc ttcagatgcg acggatcgtc
      421 tccctgaaga ccacgcacgt aagccacggc catgcgcccg gtaagatagg gatcttcgct
      481 gaagacctcc tggatgcgtc cccagcgcgg attgcggacg aggttgatga cgggcgagcg
      541 gtagaccaat cccatcgggc cgcgcggacc ttccgcgtgc gcgttgtaga gggcgcgagc
      601 ttcatcgctc atggccgcgg cgacgctgtg aaccagatcc ggatcccacg tggcgcccat
      661 cgcaatgggc gcggggaaaa gtgtggtggg ctctttcgac cagacgccgt gcagggtttg
      721 attccagcct ccccacatcg gtatgccgag gcgcggaagg ccgtggttcg tgtggttcag
      781 ctgttgggct ttttcttcca gcgtcaggcg atggatgaga tcggtgatgc gctcctcgat
      841 gggaaggcgg gggtttctga agggagcagc agacgtatct tgtccatgcg ccagcgcgac
      901 gagagctgca aaaacaagaa cagctaccag atttcgcatc gggatgctct tttcttaggc
      961 caaaggttcg attcggattt ccgcactcgc tcgatcggag ggcggaacga atgatcagag
     1021 attcacttca ccggtttatg tgcaccggcg cgcaccgttt ccgcgaccgc ggacatatcc
     1081 ttttctccga gaccctcctc ctgggctttg cggaaaagct cttccgccgg agcagaggtg
     1141 gacaatggaa cgccttcgcg tgcggctgcc tcgcgcgcat agcgaaggtc tttcgccatg
     1201 agccggagca ggaaattcac ctcgtaggtg cggcgcgtca tgcgatcggc catggcggtg
     1261 agaatgccgc tgcccggagc gccctttttc aggaactcga aggccgattc gcgtttcagg
     1321 ccgctgcgct cgatccaggc cagagcttcg gcgaacgagg tgacctgcac gccgcagaga
     1381 aaattgttga tgagtttcaa ctgcgttcca ctccccacct gtccgaggtg cacaacctct
     1441 ttgctcatga cgcgcaggac cggctcgacg gcggcaagat cggcatcatc ggcgccgacg
     1501 atgaaggtca gctgtccggc ttcggcctgg ggccggctgc ccgtgaccgg cgcttcgacc
     1561 atgcgcaagc cacgatcgcg agccgcacca tgcagctccg cgatccatgc cgggctcaag
     1621 gtgctgcact cgacgagaac tctgtctggc tgcatggcgg cgagcgctcc g
//
LOCUS       DASZYN010000693        15820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1067181, whole genome shotgun sequence.
ACCESSION   DASZYN010000693 DASZYN010000000
VERSION     DASZYN010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 15820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15820
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067181"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..136
                     /locus_tag="VHE33_17945"
     tRNA            60..136
                     /locus_tag="VHE33_17945"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:94..96,aa:Pro,seq:cgg)
     gene            complement(380..1897)
                     /locus_tag="VHE33_17950"
     CDS             complement(380..1897)
                     /locus_tag="VHE33_17950"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HVT99391.1"
                     /translation="MRYSVHSPAVRLAAFIGLVFSPALIRPCPAQTGLPQLPDPGSTM
                     VSRQQQQQLGLQAAGEVYKQMPVLPDNSPETAYVREIGQRLVAVIPPANSWPFQFHVV
                     AQKEINAFALPGGQMFVNIGTITAASNEAELAGVMAHEMSHVYMQHSAKQMQKAQLTQ
                     GIAGLAGAILGEKGGSLASLGQTGLQIGAGMVMLRYSRTDEAQADSVGAIILQRAGYN
                     PQALANFFRKLEAQGGAGPQFLSDHPNPGNREAAIQKQIQGWPPEQYRTDNAGFETAR
                     QKALSVKAYSADEIAEGAKAGRWTALNKSNGAIINPPPGESASETAQSSPGATTVNPV
                     SASGPVLWNKVAPSRNYVLSDLGLVRMVRPQNWNVIAPQQQGQSVTIAPQDGVVGAGI
                     GYGVVVNAVTLKSISIDDATAQIVRGLQSGGSDLQPVGKAAVYKVAGASGRSVILQST
                     SPFLDAHGQPQKEKDHLVTIPWENNTVIYMVFVAPAQDFERLRPAFQRMLDSVQF"
     gene            2535..3050
                     /locus_tag="VHE33_17955"
     CDS             2535..3050
                     /locus_tag="VHE33_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99392.1"
                     /translation="MYAESIANHNREIALTLIRRALDTNHDGTLEPRERAAAQIVLFG
                     QSWGGAAAIATARELEKLGVPVLLTAQIDSVGMHDAVVPRNVRAAVNFYQHDPFTIRG
                     RGGIRAADPHRTKILGNFKSSYAGRRMDESNASWQRRVFGRSHTKMELDPVIWGEVES
                     CILDAIARRKP"
     gene            3047..5035
                     /locus_tag="VHE33_17960"
     CDS             3047..5035
                     /locus_tag="VHE33_17960"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HVT99393.1"
                     /translation="MIRAALLGLLLSVPLWAQSRDFDVTPSGGWLDTGMDVNAGDTLH
                     VTATGQLQYTNARQPNGPAGLPRAFTDVLRALAVNDAGRGALVGRIGSSEAARPFLVG
                     ELLNYAVPAPGRLFLAVNQSTLDQATGSYHVVVQLTAAARTAKSEVKVPAFPQSLIDS
                     IPRRVIDADGNPGDRVNFVLIGSLDQVQAALKKAGWVTVDKTNKDAALGGLFASLSKQ
                     AYVTMPMSVLDLYGRPQDFGYAQADPLRVVASRHHFRLWKVPATLEGQTVWAGAGMHD
                     IGFARDTRNNGITHKIDPNSDNERDYIRDSLMQTGLVVKTDYITAADPVLTAKTATGS
                     EFTSDGRTLLVYLAAATGDLSATFADTFCSVLEQENPDTGSWGPCSQYLDSPGRKDLK
                     LGPLSTKYRVLIVPGIMSSCVADTPAFKEGQDALKKVGLDVALLNIPNDSSESNAKVI
                     AQYLRDHKSSDGTKYILVGYSKGGPDIQVALATEQGVSEQVAAFVTVAGASGGSPVAD
                     LLPQIADKYMKTVPIKSCQGDLSSGYKSLQRETRRAFLNAYPNPVVPTYSIIAKSDQN
                     TTSKSLLQTWRILQSFGNAEDGQLLREDAIVPGAKFLGAALADHFAIALPFDKSTDSA
                     IRSQMDKTAYPRAALLESLVRFVTADLETRTSQQIAQQ"
     gene            5869..9039
                     /locus_tag="VHE33_17965"
     CDS             5869..9039
                     /locus_tag="VHE33_17965"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVT99394.1"
                     /translation="MGGQIPIRIDLFGSLRFTFGTEVIAGVRTNRMRSLLAFLVLNRD
                     APQSREHIAFLLWPESDQAQARTNLRQLLHNLRRALPVECSLLSSDNASVRWLPDPSC
                     TVDVFEFEEAIRRAERASQDEPQALRRALEHALALYQDDLLPDLFDDWLRPKREQLRL
                     QFADALSRVAELLEAAGETTASIRHVARLIALDPLREAYYQTLMRLHLRNHDRSSALR
                     VFHQCKRSLQRELGVSPAKATQDIFMEALKSEDALRSAVAVPPPMAPTKPLPLVGRRQ
                     EWDRLSECWRRVTQGESHVILITGEPGIGKTRLAEEFLVRCSQQSGQTTATARCYRVQ
                     GQLAYGPVTEWLRTEPLIHAREKLPRPQLSELARLVPEILTETPGLASPQPLTDMWQR
                     HHLHDALTSTFRLAPKPLLLLIDDLQWCDQDSFEWLLSLFRSAPSDRLLVIGTVRAGD
                     AGRTHPLAGLKQDLSLRGQLSEIAMEPLDADETAALAMQTATREIEPAVLHRLYEATK
                     GNPLFVVESIRASLDEQLSERVPPRIQAVISARLAQLSAPAYELAGLAAAIGRSFTTE
                     LLAKATDWDEDSVSGALEELWRFRIIDGHDGVAYDYTHGILRDVAYAEVNPIRRRLLH
                     RRIARALTEIYSPDFEPLSGWLAGHFEAAGMAEEAIRFYKEAASVAKLRFADAEAASL
                     IRRALALCLGYPESARRDREELELLVTLGASLVKTDGYTMPEVGETYERGLTLSRRCD
                     DRTHVFPLLSGSWAFHIVRGQVEDSRRVAQDFVDGARRHGASGQEGASHFLLGTSLFH
                     LGKIQDSWKEMELAGSSSDGTPHPALALFAGPDIGVFRCAYLSHLHCLFGRSEEAVAK
                     SHECIAVARQLSHPFTLAIALDYAAMFDALCGEATTAQDHAGEASAICRKFGFIYYLA
                     VADILGGWARAMLGNAASGTAQMRLGFDALKSVGAELRLPFYHGLLAEACERAGKMGD
                     ALAHVATGMAFLNKNSEAWAAPELYRIQGDLFLRSGNPAQSGVSYRRAVDLSRQMGAR
                     HFERRASARLRTVSASAGEQEICVER"
     gene            9126..10532
                     /locus_tag="VHE33_17970"
     CDS             9126..10532
                     /locus_tag="VHE33_17970"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HVT99395.1"
                     /translation="MNQEAITSFRTQLRGELIEPTDAHYDESRKVYNAMIDRRPRLIA
                     VCADTADVIAAIHFARQHGLQVSIRGGGHNAGGLGVCDDGLVIDLARIRYVHVDPAAR
                     IVRVGGGATWADVDHATHAFGLAVPCGIVSSTGVGGLTLGGGIGYLTRQYGLTIDNLL
                     AADVVLADGRFVTASADENSDLFWAIRGGGGNFGVVTSFLFQAHPVSIVCAGPMLWEL
                     DQAADIMKWYREFIVQAPEQINGFFAFLTVPPGPPFPTHLHLRKMCAIVWCFNGELDQ
                     ANQILEPLRSFRPPAFEFFAPMPFPMLQGLFDALLPTGLQWYWKGDFVDELSDEAIAL
                     HIEHGSQLPTMQSTMHMYPINGAAHRFQQNDTPFSYRRSTWSTVIVGVDPEPANKDRI
                     TNWARSYWEALHPYSAGGAYVNFMMDGEGQDRIRATYQDNFARLVQVKTKYDPENFFH
                     FNQNIRPQVPTEDTALAP"
     gene            10660..11448
                     /locus_tag="VHE33_17975"
     CDS             10660..11448
                     /locus_tag="VHE33_17975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99396.1"
                     /translation="MEYGECAFVSLSHLLRMAVLSQRAVDDATKAYELGDPRFCLQLQ
                     DTEHSLRDLQSLVAERGRTLQAAGSLLDDHSVPASCTLRIYSALQITFAAAREIAHNA
                     SVIQENRECAECAPPRWREAARFANSLVTLNAVALINKQRSAAALVLRSWHHPGWPCR
                     HALPAGEPAMRQIDANAEPEECVALCLTEIADQAFEIARSIVRWLASEGCPDTTGESA
                     ISLRQVSAERWMPCTAMPSLITSNGNMGALGFAQGRFVGESGTT"
     gene            complement(11550..13253)
                     /locus_tag="VHE33_17980"
     CDS             complement(11550..13253)
                     /locus_tag="VHE33_17980"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HVT99397.1"
                     /translation="MNRRSFVGLTAGALFGGTLPEDGSTAPQSAPRLRFTQTRTGAGP
                     FEEKTGARPPHILLISADMVGPDLYHPNRPFSRHVQLPAIRSLMQDGTFFSNAFCTVP
                     LCSPSRASYLTGRYSYIQGNSERAPEGLESELRPDDVIFPEYLRAAGYVARQVGKCHV
                     GSKKFLDAFGNNDQPWDRWSPPVFDDDDFLTYQRSLGVKPQKYSREIVFRLQDRKTSG
                     NSAGGWVVQQDGRPFPMEAQYSYYLGRKAIETVEGLKTRGVLDRHPLYLQLDIFDPHQ
                     PFSIPAGFEQREQELRQAIGLPQTYKDVQARNFQRAADEPEITDIYRRYWGIYDPQAL
                     IDYRVAYALQMELVDHVIGMVIAKLKELDLYDQTLIAFISDHGEMNGRRAIVDKGVYL
                     YPDIVRVPMVFKLPASTPREHAVVESPASLLDLSQTLLDAAGIRPEAKFDGMSLLPAI
                     AGGQGEEKRTLLFFGGWHVGVNFDCGIQHKAADGHRYLYSYNCTSMVDELFDLDSEDA
                     TNLIGNPDLAGVRKEMIRQLGAALSSDPRWVGYWSEFRIAHFDDLPRTQGDMQLFESG
                     S"
     gene            complement(13311..15467)
                     /locus_tag="VHE33_17985"
     CDS             complement(13311..15467)
                     /locus_tag="VHE33_17985"
                     /inference="COORDINATES: protein motif:HMM:NF028477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4965 domain-containing protein"
                     /protein_id="HVT99398.1"
                     /translation="MQNGERGRNDSWGARRDCTPRQRKKFLQTLLVLTLTGSALPAVA
                     QEPVTARTPATPLVVHDPYFSVWSFDDTLNTEPTRHWTGTPQQLDGIVQIDGHPFRFM
                     GAEGDVPALPQQSRTIWPTRTIYEFEGDGIHLTATFLTPALPRDLDVLSRPLTYLTWS
                     VRSTDGQSHAVRLWVSASAQLAVDNPQETVVWNTAHVGDMIVMQMGDNQQPMLQKAGD
                     NLRIDWGWADLAVPAQPGMSTAILGAREFTRDATSTSAAKNDDLDMPRAARLDLPMMT
                     VSFDLGAVGSASVTRRAMLAYDDREAIEYLHRQLPDYWRRNGQTFAQMLEVAEHDQDT
                     LSTRSEAFDRAITSDLTAVGGANFAQLAILAYRQTLAAHKLVVDIDGQPLVFSKENSS
                     NGCTDTVDVTYPAAPFFLLLNPKLLEAELRPVMDYASLPRWPWPFAPHDIGTYPLANG
                     QVYGGGERTEEDQMPVEESGNMLILFDALAKAEGNADFAAHYWPQLSKWAAFLLKFGM
                     DPENQLSTDDFTGHLAHNANLSIKAIVALGAYAQIADLLHKNDEAARFRQSAQQMATQ
                     WMKMAADGDHTRLAFDQPGTWSQKYNLVWDRILGLHLFPDSLAQGELRFYLAHQNAFG
                     LPLDNRHTYTKLDWSVWTATLSSNRQQFDALIDPLYKFMTETPTRVPLSDWFDTVTGA
                     KSGFQARSVVGGVYIPMLADPAMWKKWSAGEPLKPK"
     gene            complement(15639..>15820)
                     /locus_tag="VHE33_17990"
     CDS             complement(15639..>15820)
                     /locus_tag="VHE33_17990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99399.1"
                     /translation="LGEQVLTGGTYSWTGPGGFTGTTRVVTGVPLSSGTNVFTLTYTN
                     TVGATSTATYTITAH"
BASE COUNT         3137 a         4650 c         4812 g         3221 t
ORIGIN      
        1 cgtttgcccc ggcgcatggt gtcgagtaaa ctgaagcagc gcacgccgca gcgcctcgtc
       61 ggggagtggc tcagcctggt agagcacctg gttcgggacc agggggtcgg aggttcaaat
      121 cctctctccc cgaccattta aatcaacaat ataggcttga gcctgagggt cggaactcgt
      181 cgccctctgt gtgagcgcgt tcctccgcga ccgccaatac ggccaaagcc ccggctcgtt
      241 ggcgaaacac gaccagatct cccgcgcccc gatccggccc ttccacaagg tgtgggttag
      301 atgacaacct gacgcagatt cgtgtgagct ttgcccgttg tgcttcattt ccaggtcagg
      361 cgcttcctcg ctggcaatcc taaaactgca cgctgtcaag catcctctgg aaggctggac
      421 gcaggcgctc gaaatcctga gcgggagcaa caaagaccat gtagatgacc gtgttgttct
      481 cccatggaat ggtcaccaga tggtccttct ctttctgggg ctgtccatgt gcgtctaaaa
      541 atggcgaggt cgattgcagg atcacggacc gcccgctcgc cccggcgacc ttatatacgg
      601 cggctttccc aacgggctgc aggtcactcc ccccgctctg caggccgcgc acgatctggg
      661 cggttgcgtc gtcaatgctg atcgatttaa gggttacggc attcaccacg acaccgtagc
      721 caataccggc acccacaacg ccatcctggg gtgcaattgt gacggattgc ccctgctgct
      781 gtggcgcgat cacattccag ttttgagggc gcaccattct cacgaggccg agatccgaga
      841 gcacgtaatt gcggcttggt gccaccttgt tccataaaac aggacctgac gccgaaacgg
      901 ggttcaccgt tgtggcgccg ggactcgatt gagctgtttc ggaagccgac tcacccggcg
      961 gcggattgat gatcgcgcca ttgctcttgt ttaaggctgt ccaccgcccc gcctttgcgc
     1021 cctccgcgat ctcatctgca gaatatgctt tgacacttag agccttctga cgagccgtct
     1081 cgaagccggc gttatcggtc cggtattgtt ccggtggcca tccctgaatc tgcttttgaa
     1141 tggccgcctc tcgattgcca ggattcggat gatcgctcag aaactgcggg cctgcaccgc
     1201 cttgtgcctc gagcttcctg aaaaagttgg ccagagcttg tgggttatat ccggcccgtt
     1261 gaagaatgat cgcccccacc gaatcggcct gagcttcatc ggtccgcgag tacctgagca
     1321 tcaccatacc cgcgccgatc tgaagaccgg tttgtccgag ggaagccaga ctgcctcctt
     1381 tttccccaag aatggctccg gctaagcctg ctataccctg tgtcagctgg gccttttgca
     1441 tctgcttcgc tgagtgctgc atgtacacat gcgacatttc atgcgccatg acgccggcca
     1501 gttcagcctc attggaagcg gcagttattg ttcctatgtt gacgaacatc tgtccccctg
     1561 gaagcgcaaa ggcattgatc tccttttgtg ccaccacgtg gaactgaaag ggccacgaat
     1621 tcgccggagg aatgaccgca accagccgct gtccaatttc gcgaacgtat gccgtttccg
     1681 ggctgttgtc gggcagcacc ggcatttgct tatagacttc tccggcggct tgtaatccga
     1741 gttgctgctg ctgttgacgg ctcaccatcg tgcttcccgg atcagggagc tgcggaaggc
     1801 cggtctgtgc cggacacgga cgtatcagcg caggagaaaa caccagcccg atgaatgctg
     1861 cgagcctcac ggcggggcta tggactgaat atctcatttg agtttgcctc gatcgaatgc
     1921 aaggcttcat tgctgccctc gtgttcccgg gtcttcactt ccgccacgcg agtgcgctca
     1981 caatgacagc gcttctcgcc aggagcccat ggcttgtttc cgttgtccgg gataaccggc
     2041 ccattcagca tgccagggag cgacgcccaa aatgagtttg cacgcgtggg attcaccatg
     2101 gagcatacac aaatattgga cttcgatcac atggaaaagc ctgcggcatc gctcccttct
     2161 ccgagctccg gccagggcta cactgactgc atcgcctgca tggaatcagg tctcaggggt
     2221 cggttttgac ttgcaatgcg tctgatcttg cttcgtcacc tccgcgatga acgcgcgtca
     2281 ggactgcatt gcaggtctgt cttgttgcag gagcatcgat gttcaaactg gccagctcgt
     2341 ggaaatgggc ttccatctgg gggacattgt gctgctctat gctgtctgcg cagagcatcg
     2401 ccgatatgac cacaccgacg cctcttccgc caggatcgac cctgattatc ggattcctcg
     2461 ggggatacga gcgctggaac gatgaacaca gaagcgttcg ccggctggtg ctcaggctca
     2521 ggcagcagcc gggtgtgtac gctgaatcga ttgcaaatca taaccgcgag atcgcgctga
     2581 cgttgattcg ccgagcgctc gacacaaacc atgacggcac actcgaaccg cgcgagagag
     2641 cggcggcgca gatcgttttg tttggacaga gctggggcgg tgcggcagcc atcgccaccg
     2701 cacgtgagct cgaaaagctt ggtgttcccg tattgctgac cgcacagatc gacagtgtcg
     2761 gcatgcacga cgccgtggtt cctcgcaatg ttcgtgcagc cgttaatttc taccagcacg
     2821 atccgtttac gatccgggga cggggaggaa tccgtgcggc cgacccgcac cgaacgaaga
     2881 tcctgggcaa tttcaagtcg agttacgctg ggcgccgaat ggatgaatcg aatgcatcgt
     2941 ggcagcgacg agtcttcggg cgtagccaca caaaaatgga actggacccc gtaatctggg
     3001 gcgaggtgga aagctgcatt ctggatgcga tcgcaaggag gaagccatga ttcgtgcagc
     3061 gctgctgggt cttttgcttt ccgtgccgct gtgggcgcag agccgggatt tcgatgtgac
     3121 gccgtccggc ggatggctgg ataccggcat ggatgtgaat gccggcgaca cactgcatgt
     3181 gacggcaacc ggacaattgc aatatacgaa tgctcgccag ccaaatggtc cggcgggatt
     3241 gccccgcgct tttaccgacg ttctgcgtgc gctggcggtg aacgatgcag gccgtggcgc
     3301 gctggtaggg cggatcggat cgagcgaagc agcgcggccc ttccttgtcg gtgaattgtt
     3361 gaattacgct gtgcccgctc cagggcggct ttttctggcg gtcaaccagt caacgcttga
     3421 ccaggcaacc gggtcctatc acgtcgtggt gcagcttacc gccgccgcac ggacagcgaa
     3481 gagcgaagtg aaggtgcccg cttttccgca gagcctgatt gattcgattc cgcgccgtgt
     3541 catcgatgcg gacggcaatc ccggtgaccg ggtaaatttc gtcctcatcg gttcactgga
     3601 tcaggtgcag gctgcgctga agaaagcggg ctgggttacc gtcgacaaaa ccaacaaaga
     3661 cgccgcattg ggcggactgt ttgcaagcct gtcgaagcaa gcctacgtga ccatgccaat
     3721 gagcgtgctg gacctgtacg gacgccccca ggattttgga tatgcgcagg ccgacccgtt
     3781 gcgggtggtg gcgtcgaggc atcattttcg gctgtggaag gtgccggcaa cgctggaagg
     3841 gcaaaccgtc tgggccggcg ccggaatgca cgacatcggc tttgctcgcg acacgcgcaa
     3901 caacggaatc acgcacaaga tcgacccgaa cagcgacaac gagcgcgact acatccgcga
     3961 cagcctgatg caaaccggac tggtggtcaa gacggattac atcacggcgg ccgatccggt
     4021 gctgaccgcc aaaaccgcga cgggatcgga gtttacttcg gacggccgca cgctgctggt
     4081 ttacctcgcc gccgcgactg gagatctgtc cgctacgttt gccgacacct tctgctcagt
     4141 tctcgagcag gagaatccgg atacgggaag ctggggtccc tgctcacaat acctcgactc
     4201 gcccggcagg aaagatttga agctgggacc gctttcaacg aagtatcgcg ttctgatcgt
     4261 gccgggaatc atgagttcgt gcgtggccga cactcccgca ttcaaggagg ggcaggatgc
     4321 gctgaaaaag gtcggccttg acgtggcgtt actgaatatt ccgaacgatt caagcgagtc
     4381 caacgcaaaa gtgattgcac agtatctgcg cgatcacaag tcgagcgacg gcacgaagta
     4441 catcctcgtt ggctacagca aaggcggacc ggacatccag gtcgcgctgg ccacggagca
     4501 gggtgtgagt gagcaggtgg cggcatttgt taccgttgcg ggcgcatcgg ggggatcccc
     4561 ggtggccgat ttgctgccgc agatcgctga caaatatatg aagacggtac cgatcaagag
     4621 ctgtcagggc gatctctcga gcgggtacaa gagcctgcaa cgggagactc gcagggcctt
     4681 tctgaacgcg tatcccaacc ccgttgttcc gacttattcg atcattgcga agtcggacca
     4741 aaacaccaca tcgaagtcgc tgctccagac ctggcgcata ttgcagagct ttgggaacgc
     4801 agaggacggc cagcttctgc gggaggatgc aattgtgccg ggcgcgaagt ttctcggggc
     4861 ggcgcttgcc gatcacttcg cgattgcgct gccgttcgac aaatcgaccg attctgccat
     4921 tcgttctcag atggacaaga cggcgtaccc ccgcgccgcc ctgctggaat cactggtgcg
     4981 gtttgtgacc gccgacctcg agaccaggac cagtcaacag attgcgcagc aatgaacagt
     5041 ggccctgcag tgacgaccgg ctatgcctgc gggcgccttc cgtcgcgctg agctgctgag
     5101 ctgtttctga atggccggag cgataagatc cggccgttcc acagggtgtg agttagcgaa
     5161 caccttcagc ccactgtcca gctccctaac tcaataattg ggtcaagcct gtacgccaac
     5221 agatgacgat ccttgcgagg aagaccatct cctgcatgcc gaggacggat gacaggaatg
     5281 acgaacgaat cgtatcgttg cgggcaactg ggctttaccc tgccgcgcct cgaatggcaa
     5341 gtggagaagg cgtgagcacg gtggtgccgt cctgctggtc gaagacttgc gccgtctcgg
     5401 cgatctcgtc agcggacaga aggccgtttg ttaatgactc cgacgaatgg actcgctcat
     5461 cgcaatcgag cgctgcgaga ggataagtcc tgggaaaagg ggcttcaggc ctgcgagttc
     5521 ttcccgatcg ctcgaatccc gattctctgt cggtctgaac cggcaaactc ccataagaca
     5581 cgaaaaccgc tcgattgcat gagcaggaat tgtcttttcc cgcgggcaat tggtcgagcc
     5641 tggttctggt agtatcaggc acagatacgg atcgaatcgc gccgctatgc atgtcacccc
     5701 agcagctgta taacgggcgc tgtattccag ttctttactt ctcgctcatc gatctgttca
     5761 atcggcaatt cgggactctg tcctcgtgga gaggcggggc ggcaatcgct tcaccgtatc
     5821 aggacctctg gaggacaatc cgtttcgagg gcccaaatgg gagttcctat gggcggacag
     5881 atccccattc gtatcgattt atttggcagt ctgcgcttca ccttcgggac ggaagtaatc
     5941 gcgggggtca ggacgaatcg gatgcggtcg ctgctggcct ttcttgtcct caatcgggat
     6001 gcaccgcagt ctcgcgaaca catcgctttc ctgctgtggc cggaatcgga tcaggcccag
     6061 gcgcgaacga atctgcgaca actgctgcat aatctgcggc gggctctgcc cgtcgaatgc
     6121 tctctcctca gcagtgacaa cgcctccgtc cgctggcttc ctgacccctc atgcacggtc
     6181 gacgtgttcg agttcgagga ggctatccgc agggcagaac gcgcgtctca ggatgaacct
     6241 caagcgctgc ggagagctct cgagcatgcg ctggcgctct accaggatga cctgttgccc
     6301 gatctgtttg acgactggct gcgaccgaag cgggaacaac tgcgcctgca gtttgccgat
     6361 gctctcagcc gcgtcgctga actgctggaa gcagccggtg aaaccacggc gagtattcgg
     6421 cacgtcgcgc ggctgatcgc cctcgatccc ctgcgagagg cgtactacca gactctgatg
     6481 cggctccatc ttcgcaatca tgatcggtcc agcgcactgc gtgtctttca ccaatgcaag
     6541 cgcagcctgc agcgggaact cggcgtgagt ccggccaaag cgacccagga cattttcatg
     6601 gaagcactca agtccgaaga cgccctccgg agcgcggtgg cagtcccgcc tccgatggcg
     6661 cccacaaagc cgctgcctct ggtggggcgc cgtcaggagt gggatcggct ctcagagtgc
     6721 tggcgtcgcg tcactcaagg tgaatcccac gtcatcctga tcaccggcga acccggtatt
     6781 ggcaagacgc gcctcgccga agaatttctt gtccgatgtt cccaacagtc cggtcagacg
     6841 accgcaactg cgcgttgtta tcgggtacag ggccaactag cctacggtcc ggtgaccgaa
     6901 tggctgcgaa cggagccact gatacacgca cgtgaaaaac tgcctcgccc tcagctttcc
     6961 gagctggcgc gactggtgcc cgagatcctg acagaaactc ccgggctggc atcgccgcaa
     7021 cctctcacag acatgtggca gcggcaccat ctgcacgacg ctttgacctc gacgtttcgg
     7081 ctggccccaa agccgctgtt gctgttgatt gacgatttgc aatggtgtga tcaggattcg
     7141 tttgaatggc tcctttccct gttccgatcg gcgccttcgg atcgcttgct tgtaatcgga
     7201 accgtgcgcg caggggacgc cgggcgaact cacccgctgg ctggcctgaa gcaggacctg
     7261 agtctgcgcg gacaattgtc ggaaattgcc atggagccgc tcgacgcgga cgagacagcc
     7321 gcgctcgcga tgcagacggc gacgcgcgaa atcgaaccgg ctgtcctcca ccgcctctat
     7381 gaagcaacca aaggcaatcc tctgttcgtc gtggagagca ttcgagcctc tctcgacgaa
     7441 cagctgtccg aacgagtgcc gcctcgcatt caggctgtta tcagtgcgcg ccttgcgcag
     7501 ttatcggcac ccgcttatga attggccgga cttgcggcgg ccattggccg atcgttcacg
     7561 acagaactgc tggccaaagc aacagactgg gacgaggaca gcgtatcggg cgctctggag
     7621 gagttatgga gatttcgcat cattgatggc cacgacggag tcgcttacga ctacacgcat
     7681 ggaatactcc gggatgtggc gtatgcggaa gtcaatccca ttcgcagaag attgctgcac
     7741 cggcgcattg cccgtgcgct caccgagatc tattcgcccg attttgaacc gctgagcggg
     7801 tggctggcgg gacactttga ggcggcaggg atggcggagg aggcaatccg cttctacaag
     7861 gaggcggctt cggtggccaa actgagattc gccgacgcgg aagctgcgag cctgatccgt
     7921 cgcgccctgg cgctttgcct tggctatccg gagtcggcgc ggcgagacag agaggaactc
     7981 gaacttctcg tcacgctggg cgcttccctg gtgaaaacgg acggctacac catgcccgag
     8041 gtgggtgaga cgtacgagcg agggcttacc ctgtcaagac ggtgcgacga ccgcactcat
     8101 gttttccctt tgctgagcgg atcgtgggct ttccatatcg tgcgggggca ggtggaggac
     8161 tcaaggagag tggcgcagga ttttgtcgat ggggcgcgcc gccacggcgc ttccggacag
     8221 gaaggagcaa gtcactttct gctggggaca agcctgttcc atctggggaa aatacaagat
     8281 tcatggaagg agatggaact ggccggttcc tcgtccgacg gaactcccca tccggctttg
     8341 gccctgtttg ccggaccgga catcggcgtt tttcgctgcg cttatctgtc ccatctgcat
     8401 tgtctttttg gccgtagcga agaggctgtc gcaaagagtc acgaatgcat tgcagttgca
     8461 cgccagctct cgcacccttt tacgctggcg attgcactgg actacgcggc aatgttcgac
     8521 gccttgtgcg gagaagccac tacggcccag gatcatgcag gcgaagcttc ggcgatctgc
     8581 cggaaatttg gattcatcta ctacctggca gttgcggata ttcttggcgg ctgggccagg
     8641 gccatgctcg gaaatgccgc atcggggaca gcccaaatgc gcctgggttt cgacgctctg
     8701 aagtcagtcg gcgcagagct tcgcctgcct ttctaccatg ggctcctggc ggaggcctgc
     8761 gaacgggcag gcaaaatggg ggacgcgctc gcccatgtcg ctaccggcat ggctttcctc
     8821 aacaagaact ccgaggcctg ggcggcgccg gaactgtacc gaattcaagg agatctgttc
     8881 ctgcggagcg gcaatccggc gcagtccgga gtaagctatc gccgcgcagt cgatctcagc
     8941 cgacagatgg gcgctcggca cttcgagagg cgggcatccg cccggctgcg cactgtttcc
     9001 gcatcggcag gcgagcagga aatctgcgtc gaacggtagt tcctgccccc gcagaacgct
     9061 ttccgaacgg tccttcccgc aaagtgatac ttgagccgct gacattccat ggcccagagg
     9121 agcttatgaa tcaggaagcc atcacatcct tcagaaccca gcttcgcggt gaattgattg
     9181 agcccaccga tgcgcattac gacgaatcgc gcaaggtgta caacgccatg atcgaccgca
     9241 ggcctcgact cattgccgtc tgtgccgaca ccgccgatgt gatcgctgcc attcacttcg
     9301 cgcgtcagca tggtcttcag gtgtccatcc ggggtggcgg ccataacgcc ggggggctgg
     9361 gagtctgcga tgacggtctg gtcatcgatc tcgcgcgaat ccgctatgtc cacgtcgatc
     9421 cggcagcgcg aatcgtgcgc gttggcggcg gcgccacgtg ggccgatgtt gaccacgcca
     9481 cgcacgcctt tgggctcgca gtgccttgtg gaatcgtctc atcgaccgga gtcggtgggc
     9541 tcacgctggg cggcggcatc ggatatctga caaggcagta cgggctgacc attgacaatc
     9601 tcctggccgc cgacgtcgtt ctggcggacg gaagatttgt caccgcgagc gcggacgaga
     9661 acagcgacct gttctgggcg atccgcggcg gcggggggaa cttcggcgtg gtcacctcgt
     9721 tcttgtttca ggcgcatccg gtttcgatcg tgtgcgcagg ccccatgttg tgggaactgg
     9781 accaggccgc cgacatcatg aagtggtatc gcgagtttat cgtccaggca cccgagcaaa
     9841 tcaacggctt cttcgcgttt cttaccgtac ctccggggcc accattccca acccacctgc
     9901 atttgcggaa aatgtgcgcg attgtatggt gcttcaacgg agaactcgat caagccaacc
     9961 agatccttga accgctgcgc agcttccgcc cgccggcctt cgaattcttc gcgccgatgc
    10021 catttcccat gctgcaggga ttgttcgacg cacttctgcc aacgggtctg cagtggtact
    10081 ggaaagggga cttcgtcgac gagctgagcg acgaggcgat tgctctgcat atcgaacatg
    10141 ggtcccaatt gcccaccatg caatcgacga tgcacatgta ccccatcaat ggagcggcgc
    10201 accgttttca gcaaaacgac acgccattca gttatcgcag gagcacctgg agcaccgtca
    10261 tcgtcggtgt cgatccggag ccggcgaata aggatcgcat caccaactgg gcccgcagct
    10321 attgggaagc gctccatccc tatagcgcag gcggtgcgta tgtcaacttc atgatggatg
    10381 gcgagggaca ggatcgcatt cgcgcgacct atcaggacaa tttcgcgagg ctggttcagg
    10441 tgaagacgaa atacgatccc gagaacttct ttcacttcaa ccagaacatc cggccacagg
    10501 tgcccaccga ggacactgca ttggcgccgt gatgaagcta aacaggattt cggatcatcg
    10561 gtaccagctc ttatcgtccg aagatccgcc cgccggctca gccctctggg cggttgaaga
    10621 tgcagtgctg gtccggaatc aggaaggaga taaacaacca tggaatacgg agagtgtgct
    10681 tttgtctcac tttcgcacct gctcaggatg gcggtgcttt cacaacgtgc tgtcgatgat
    10741 gcgaccaaag cttatgaact gggcgacccc cgattctgtc tccagctgca ggatacggag
    10801 cacagcctgc gggatcttca aagcctggtc gcggagcgcg gacgaactct acaggccgcc
    10861 gggagcctcc tcgacgatca ctcggtcccg gcgtcctgca cactgcggat ttatagtgcc
    10921 ctgcagatca cgttcgcggc ggccagggag atcgcacaca acgcgtcggt cattcaggag
    10981 aaccgagagt gtgcggagtg tgcgcctccc cgctggcggg aagcggcaag attcgccaac
    11041 agcctggtca cgctgaatgc cgtggccctg atcaacaaac aacgcagcgc cgcagctctg
    11101 gttcttcgca gctggcatca tccaggatgg ccgtgcaggc atgcccttcc tgcaggggaa
    11161 ccggccatgc ggcaaatcga cgccaacgcc gagcctgaag agtgcgtggc tctgtgtctg
    11221 acagagattg ccgatcaggc cttcgagatc gctcgttcca ttgtgcgctg gcttgcgtcg
    11281 gaaggctgcc ccgacacgac cggtgaatct gccatctctc tgcgacaggt gagcgcggaa
    11341 cgatggatgc catgtacagc aatgccatcc ctcatcactt cgaatggcaa catgggcgct
    11401 ctgggctttg cccagggccg cttcgtgggc gaatcgggta caacataaac cgtttggaga
    11461 aagagatgtc cgacaacgga acgaacctgt gcacccggtc gggcttctgt ttcatctgtg
    11521 ccagaaaacg tcgcggagcc gcctgacgct cagctgccag attcgaacag ctgcatgtcg
    11581 ccttgtgtgc ggggcagatc gtcgaagtgc gcgatccgga attccgacca gtagcccacc
    11641 cagcgcggat cggaggagag cgctgctccc agctggcgaa tcatttcttt gcgcacgccg
    11701 gccaggtctg gattaccgat gaggttggtg gcgtcttcgc tgtcgagatc gaacaactcg
    11761 tccaccatgg acgtgcagtt ataggagtaa agataacggt ggccgtcggc ggctttgtgc
    11821 tggatgccgc agtcgaagtt cacgccgaca tgccagcctc cgaaaaagag cagcgtgcgt
    11881 ttctcttcgc cctggccgcc cgcgatcgcc ggcagcagcg acatgccgtc gaatttagcc
    11941 tcgggccgga tgccggcagc atcgagcaac gtctgagaaa gatcgagcag ggaagccggg
    12001 ctttcgacga cagcatgctc tcggggcgtg ctcgcgggca acttgaagac catgggtacg
    12061 cgaacgatgt ccgggtaaag atagacgccc ttatcgacaa tcgcgcgccg cccgttcatc
    12121 tcgccatggt cggagataaa cgcgataagg gtttgatcgt agaggtccag ctctttgagc
    12181 ttcgcgatca ccatgccgat gacatgatcg accaactcca tttggagagc gtaggcaacg
    12241 cggtagtcga tcagggcttg cggatcgtag ataccccagt agcgccggta aatatcggta
    12301 atctccggct catcggcggc gcgctggaag ttgcgggctt gaacgtcctt gtaggtctgg
    12361 ggcagaccga tggcttgccg tagctcttgc tcccgttgct cgaatccggc gggaatggag
    12421 aacggctgat gcggatcgaa gatgtcgagc tggagataga gaggatggcg gtccagcacg
    12481 ccgcgcgtct tgagaccttc gacggtttca atcgccttgc ggccgaggta ataactgtac
    12541 tgcgcctcca tcgggaaggg tcggccatcc tgttgaacga cccacccgcc ggcggagttc
    12601 ccggatgttt tacgatcctg gagacgaaag acaatttctc gcgagtactt ttgcggcttg
    12661 acgccaaggc tgcgttggta cgtgagaaaa tcgtcatcgt cgaacacggg cggcgaccag
    12721 cgatcccagg gctgatcgtt gttgccaaag gcatcgagga actttttcga tcccacatgg
    12781 cacttaccga cctggcgcgc gacatagccg gcggcgcgaa gatattcggg gaagataaca
    12841 tcatcggggc gcagctccga ttcgagcccc tcaggagcgc gctcgctgtt gccctggata
    12901 tagctgtaac gtccggtgag ataggaggct cgcgaaggcg agcaaagcgg cacggtgcag
    12961 aaggcgttcg aaaagaaggt tccgtcctgc atcagagagc gaatggcggg taactggacg
    13021 tggcgggaga agggacggtt gggatgatag agatccggac cgaccatatc ggcgctgatg
    13081 agaagaatgt gcggaggccg cgcgccggtt ttctcctcga agggcccggc gccggttcga
    13141 gtctgcgtga agcgaaggcg cggcgcggac tgaggagccg tggagccgtc ctcggggagc
    13201 gttcctccga agagcgcacc agcagtcagg ccgacgaagg atctgcgatt caccgaaaag
    13261 gccccccgat tctcaaagga aacaatacat gggcgatggg aagactttcg ttatttcggc
    13321 ttcagcggct cacctgcgga ccacttcttc cacatcgccg ggtccgccag catcggaatg
    13381 tacaccccac ccaccacgga gcgtgcctga aatccggatt ttgcgccggt caccgtatcg
    13441 aaccagtcgc tcagaggaac ccgcgtcgga gtctcggtca tgaatttgta gaggggatcg
    13501 atgagcgcat cgaactgctg gcgattcgac gagagcgtcg cggtccagac gctccagtcg
    13561 agtttggtgt aggtgtggcg attgtcgagc ggcaggccaa acgcattttg atgggcgagg
    13621 tagaagcgca attcgccttg cgcgagcgaa tccggaaata ggtgcaggcc cagaatacgg
    13681 tcccacacca ggttgtattt ctggctccag gtccccggct ggtcgaacgc caggcgcgtg
    13741 tggtcgccgt cggcggccat cttcatccat tgcgtggcca tctgctgtgc gctctggcgg
    13801 aagcgtgcgg cttcgtcgtt cttgtggagc agatcggcga tctgcgcgta ggcgcccagg
    13861 gcaacaatcg ccttgatcga cagatttgca ttgtgggcga gatgaccggt gaaatcatcc
    13921 gtgctgagct ggttctccgg atccatgccg aacttcagca ggaaagccgc ccacttcgag
    13981 agctgcggcc aatagtgtgc ggcgaagtcc gcgttgcctt ccgctttagc cagcgcgtcg
    14041 aacaggatga gcatattgcc gctctcttcg accggcatct gatcttcttc ggtcctctcg
    14101 ccgccgccgt acacctggcc gttggccagc gggtacgtgc cgatgtcatg cggtgcgaac
    14161 ggccacggcc agcgcggcag gctcgcgtag tccatcactg gccgcagctc cgcctccagc
    14221 agcttcgggt tgagcaagag aaagaacggc gccgccggat atgttacgtc caccgtatcc
    14281 gtacagccgt tgctggaatt ctctttcgag aagaccaggg gctgcccgtc gatatccacg
    14341 accagcttgt gcgcggccag cgtctgccga taggcaagaa tagcgagctg cgcaaaattc
    14401 gctccgccca ccgccgtcag gtctgaggta attgcccgat cgaaggcctc cgaccgcgtg
    14461 ctgagtgtgt cctggtcgtg ctcggcaact tcgagcatct gcgcaaacgt ctgcccgttg
    14521 cgccgccagt agtccggaag ctgccggtgc aggtattcga tggcctcgcg atcgtcgtag
    14581 gccagcatcg cgcgccgtgt gacggacgca gagcccaccg ctcccaaatc gaatgagacg
    14641 gtcatcatcg gcaaatccag acgcgccgcc cgaggcatgt cgaggtcgtc gtttttggcc
    14701 gccgaagtgc tcgtcgcatc gcgcgtaaat tcccgggcac cgagaatggc cgtcgacatt
    14761 ccaggctggg cgggcacagc gagatcggcc cagccccagt cgatgcgaag attgtcgccg
    14821 gctttctgca gcatgggttg ctggttatcg cccatctgca tcacgatcat gtcgcccaca
    14881 tgggcggtgt tccagaccac agtctcctgc ggattgtcga cagcgagctg cgcgctcgcg
    14941 ctcacccaaa gcctcaccgc gtgggattgt ccatcggtcg atcgcaccga ccatgtcaga
    15001 tacgtcaggg gacgcgacag cacgtcgagg tcccggggca aggccggcgt caggaacgtc
    15061 gccgtcagat gaatcccgtc tccttcgaat tcgtaaatgg tgcgcgtcgg ccagatcgtc
    15121 cgcgattgtt gcggcagggc cggaacatcg ccctccgcac ccatgaagcg gaagggatgt
    15181 ccatcgatct gcacgatacc gtccagttgc tgcggcgtgc cggtccagtg ccgtgtgggt
    15241 tcggtattga gcgtgtcatc gaaggaccaa acgctgaagt atggatcgtg caccacgagc
    15301 ggtgtggcgg gcgtgcgcgc ggtgaccggc tcctgcgcga ccgcaggcaa cgcggaacct
    15361 gtcagcgtca ggacaaggag ggtttgcagg aatttcttcc tctgtcgcgg cgtgcagtcc
    15421 cgtcgtgctc cccaactatc gttgcggcct cgttctccat tttgcatacg gttttatccc
    15481 tcactccatt gagctgactg acccgatctc gcacaagcat tgcaaaagag tttagggtac
    15541 acgcttcctg ggaacaggtc acttcacgtc gttcctgcaa aaagcaaaac ggccggatcg
    15601 ggaagatccg gccgttgagt atcgagcata aattcaggtc aatgtgcggt gatggtgtag
    15661 gtagccgtgc tggtggctcc aaccgtgttg gtgtaggtga gggtgaagac gttcgtcccg
    15721 gaactgagcg ggacccccgt cacaactcga gtcgtgccgg taaaaccacc ggggccggtc
    15781 cagctatagg tgccgccggt cagaacctgc tcgcccagat
//
LOCUS       DASZYN010000694         3435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_945820, whole genome shotgun sequence.
ACCESSION   DASZYN010000694 DASZYN010000000
VERSION     DASZYN010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945820"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VHE33_17995"
     CDS             complement(<1..455)
                     /locus_tag="VHE33_17995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99400.1"
                     /translation="MKARIVTAGVMIWAAMAVAQTNPDQNRQPVMPTNLPDPLVFANG
                     TRVATPAQWPKRRQELLQLFSEQMYGRMPGRPAEMRFHVYDVDRHALGGKATREQIAI
                     LFRGREDGPRMDLLLYIPNAASKRPPVILGLNFWGNETVSADPGIRMSDR"
     gene            complement(546..1847)
                     /locus_tag="VHE33_18000"
     CDS             complement(546..1847)
                     /locus_tag="VHE33_18000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT99401.1"
                     /translation="MPGWAPAGMNPTSPERVGEGSPHYAGWGVLAAAFTGVMVSFAPI
                     VPYTFSLFLDPLHAAFGWKREAMGGAFALAAMTVALVSPLIGLLLDRFPPRRIILPAI
                     VAFALALASLSRLNSHIGHFYLTFFVLGLAANGTAQFAYTRTVLTWFSKHRGFALALL
                     LTGSGVGSILIPPLTQWTIQHHGWRDAWLLLGGIALLGFPLTAMLVRNRPEKPLVRTD
                     RQASGMTVAAALRTAAFWILAVITILSAFSENGLVTNLAAILTQHGVLAGSAAVALSV
                     RGGAGIVGRLLIGLAIDRVSPERIQTLVLALAGTGTLILAFAGTGPMALLGAAVLGVG
                     LGSEADVMPYLLARYFGRRHFSVLYGLTWTAYAIGGATGPLWIGHLYDRGGFYEARFI
                     VYLAGVAFAAVVLSLFLSRGRSICLSEPHEVAQTPAREFKS"
     gene            complement(1854..2393)
                     /locus_tag="VHE33_18005"
     CDS             complement(1854..2393)
                     /locus_tag="VHE33_18005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01926.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HVT99402.1"
                     /translation="MPHIALPEGLPGISAGFAFRPETAKPMRELAHILLHEPNSLPPG
                     ERELIAACVSSQNDCYFCQASHGAAAACHLGDSEIVEQVKTDVESAPVTPKLKALLKI
                     AGKVQQDGKRVSAADIAEAKAHGATDVEIHDTVLIAAAFCMYNRYVDGLGTWQPRDEP
                     MYAEMGKHLAEHGYTKPSR"
     gene            complement(2404..2973)
                     /locus_tag="VHE33_18010"
     CDS             complement(2404..2973)
                     /locus_tag="VHE33_18010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01926.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HVT99403.1"
                     /translation="MPHIALPEGLPGIRGAMAFRPETAKPLNELVEILLFGPHTLSPG
                     ERELIATYVSYLNDCHFCQSIHGAIAAAHLGGDEDLVKRVKADFQHADISDKLKALLV
                     IAGKVQRGGKNVTPADVEHARTLGATDVEIHDTVLIAAAFCMYNRYVDGLGTTQPHDE
                     AMYRERGKVVARKGYVEGSKDYMPAQAVR"
     gene            complement(3174..>3435)
                     /locus_tag="VHE33_18015"
     CDS             complement(3174..>3435)
                     /locus_tag="VHE33_18015"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HVT99404.1"
                     /translation="RELLHDGCPTACFGDVPFGYVNVFRAHVNVGFFQGAGLRDPARL
                     LEGTGRFMRHVKLRPGAAVDSAALTKLIEAAYADIKNKVENG"
BASE COUNT          676 a         1114 c         1039 g          606 t
ORIGIN      
        1 cgatccgaca ttcgaatacc tggatcggca ctgaccgtct cattacccca gaaattcagg
       61 ccgagaatca cgggcggccg cttcgacgct gcgttgggga tgtaaagcag cagatccatc
      121 ctcggcccgt cttcacgtcc gcgaaacaga atcgcgatct gctcgcgcgt cgcttttccg
      181 cccagcgcat gacggtcgac gtcgtacaca tggaaccgca tctccgccgg acgccccggc
      241 atgcgcccat acatctgctc gctgaaaagc tggagaagtt cctgccttcg cttcggccat
      301 tgcgctggcg tcgccacccg cgtcccattg gcaaacacca ggggatccgg caggtttgtc
      361 ggcattacag gctgtcgatt ctgatcggga ttcgtctgcg ccactgccat ggccgcccaa
      421 atcattacgc ctgcggttac gatccgtgct ttcatggcca tctctccttc agtggatgaa
      481 agaccagaat gccttctggt tcaaattcgc gtaaaggcga caccggcaag cagtggcagg
      541 attcgctagc ttttgaactc ccgcgcagga gtctgcgcca cctcatgcgg ttccgataag
      601 catatgctgc gtccccgcga cagaaacagg ctcagcacca cggccgcgaa agccacaccc
      661 gcaagataga caatgaaccg agcctcatag aagccaccac ggtcgtagag gtgtccgatc
      721 cacagtgggc ccgtcgcccc gccgatggcg taagcggtcc aggtgagccc atagagcacc
      781 gagaagtggc gacggccgaa ataccgcgcc agcaggtacg gcatgacgtc cgcttcgctc
      841 cccaagccga caccgagaac ggccgcaccg agcaacgcca tcggtccagt accggcgaag
      901 gccaggatca gcgttcccgt gccggcgagt gccagtacca gtgtttggat ccgttccgga
      961 gaaacgcgat caatggcgag accaatcagg aggcggccga caatgcctgc tcctccccga
     1021 accgagagag ccaccgcggc gctgcctgcc aaaacgccat gctgcgtcag gatggccgcc
     1081 aggttggtga cgaggccgtt ctcgctaaac gcggaaagga tcgtgatgac ggcgaggatc
     1141 cagaaggctg ccgtccgcag ggcagcagcc accgtcatgc ccgaggcctg ccgatccgtc
     1201 cgcaccagcg gcttttccgg ccggttgcgc accagcattg cggtcagggg aaagccgagc
     1261 agtgcaatgc cgcccagcag gagccacgca tcgcgccacc catgatgctg aatggtccac
     1321 tgggtcaggg gtggaatcag gatcgagccg acgccgctgc cggtgagcaa gagcgcaagc
     1381 gcgaaacccc gatgcttcga gaaccaggtc agcaccgtgc gggtgtaggc gaactgggcg
     1441 gttccgttgg ccgcgaggcc gagcacaaag aacgtgagat agaaatgccc gatgtgggaa
     1501 ttgagcctgc tcagcgaagc cagggcgagt gcgaaggcca caatggccgg caaaatgatg
     1561 cgccgcgggg gaaagcgatc gagcaggagg ccgatgagcg gcgagaccag cgcaaccgtc
     1621 atcgctgcca gcgcaaaagc gccccccatg gcttcgcgct tccacccgaa ggccgcatgc
     1681 agcggatcga gaaagagact gaacgtgtag gggacaatgg gagcgaagct caccatcacg
     1741 ccggtgaagg cggcagcaag aaccccccac ccggcatagt gcggcgatcc ttcgccgacg
     1801 cgttctggag aagtcggatt catccctgcc ggcgcccacc ccggcatgtg cgttcagcgc
     1861 gacggctttg tgtagccatg ttcggcgagg tgctttccca tctccgcata catcggctcg
     1921 tcccggggct gccaggtacc gagtccatcc acatagcggt tgtacatgca gaaggccgcg
     1981 gcgatgagga ccgtgtcatg gatctcgaca tccgtagcgc catgcgcctt tgcttcggcg
     2041 atgtcggcag cggagacgcg cttgccgtcc tgctgcacct tgccggcaat cttcagcagc
     2101 gctttgagtt tcggcgtgac cggagcactc tccacgtcgg tcttcacctg ctctacgatt
     2161 tcagagtccc cgagatggca ggcggctgcg gctccgtggc tggcctgaca gaagtagcag
     2221 tcattctgcg acgacacaca ggcggcaatg agttcccgct cgccgggcgg caggctgttc
     2281 ggctcgtgga gcaggatgtg cgccagctcg cgcatgggtt ttgcggtctc cggacgaaat
     2341 gcgaagcccg cactgatgcc gggcagtcct tcgggcagag cgatgtgcgg catcctgttc
     2401 tcctcagcgg acggcttgcg ccggcatgta gtccttgctt ccctccacgt atcccttgcg
     2461 cgccaccacc ttgccccgct cgcgatacat ggcctcatcg tgcggttgcg tcgtgcccag
     2521 cccgtcgacg taccggttgt acatgcagaa ggcggcggcg atcaggactg tgtcgtgaat
     2581 ctccacatct gtcgcgccca gggtgcgcgc gtgctcgacg tcggcaggcg tcacattctt
     2641 ccctccccgc tggaccttgc cggcaatcac cagcagcgct ttgagcttgt cggagatgtc
     2701 cgcgtgctga aaatcggcct ttacccgctt caccagatcc tcatcgccgc ccagatgcgc
     2761 cgccgcgatc gcgccgtgga tgctttggca gaagtggcag tcattcagat acgacacata
     2821 ggtggcgatc agctcccgct cccccggact cagcgtgtgg ggaccaaaga gcagaatctc
     2881 gacaagctcg ttgagaggct tcgcggtttc gggacgaaag gccatcgcac cgcgaatgcc
     2941 gggcagtcct tcgggtaggg cgatgtgggg catcgattgt cctttccatc cggggatcga
     3001 atcgtacttt cgccaggatc gctgcgcgcg gctggcgttc aacagccaac caaaaatctc
     3061 gccgcctggc ggaaaaggaa cggtagcagc ctcacgtaac ggaatgcacc aacgagaaga
     3121 cggggatccg tctccatcgg aagttggagt caggtcgagc cgtcgcttcc cgcttacccg
     3181 ttctccacct tgttcttgat atccgcgtaa gccgcttcga tcagcttcgt gagcgctgcc
     3241 gagtccaccg ccgctcccgg ccgcagcttg acatgccgca tgaacctgcc cgtgccttcg
     3301 agcagccgcg ccggatcccg cagccctgcg ccctgaaaga atcccacgtt gacgtgcgct
     3361 ctgaacacgt tcacgtagcc gaaaggcaca tctccgaaac acgccgtagg gcagccatca
     3421 tgcagaagtt cccgg
//
LOCUS       DASZYN010000695         3386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1007206, whole genome shotgun sequence.
ACCESSION   DASZYN010000695 DASZYN010000000
VERSION     DASZYN010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007206"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /locus_tag="VHE33_18020"
     CDS             <1..1313
                     /locus_tag="VHE33_18020"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99405.1"
                     /translation="GLSAVCFAFMTVLMSGINMYSMALVMKVVLGWDLNFSIWVSSIT
                     VAVYVALGGLRSAIFNEILQFLLIWLGALLIPILGLVEAGGWEPLKHQIAARMGSMQY
                     THLWSTTAHFRDNPMGIHWTGLVFGLGIAISCGYWTTDFLVVQRVLSAHNLRSARMAP
                     IIGAGFKMMVPFIVILPGLLALSVLPFKLVPQDIAVATGQHSYNEVLPLMLVRYCGPG
                     LLGLGVTALIAGFMSGMAGNVSAFATVWTYDIYKAFMNKKATDSHYVSMGRWCTFIGV
                     LVSVGTAYLVQHAASIMDYVQALFSFFIAPLLGTVLLGMLWKRATKAAGFWGLLAGTC
                     TAIGLFLWVHFNPAALRYVALSADAKVMAEDMYRALWSCLVCVIVTVIVSLMTQPKTD
                     AELEGLVYGASKIPEEHDEHWYQRPLVWASVVAVGFIVLNIIFW"
     gene            1321..1527
                     /locus_tag="VHE33_18025"
     CDS             1321..1527
                     /locus_tag="VHE33_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99406.1"
                     /translation="MMKGHIQIWFFSGILLLIYGVLITATGLWELSHPLANPPVLNGL
                     HAPIWWGALMVVAGLFYTLKFRPR"
     gene            1572..2990
                     /locus_tag="VHE33_18030"
     CDS             1572..2990
                     /locus_tag="VHE33_18030"
                     /inference="COORDINATES: protein motif:HMM:NF014948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HVT99407.1"
                     /translation="MKKQLTFGVIVGNRGFFPDHLAKEGREEILKVLDKAGIRAITLA
                     PEQSKFGSVETHDDAIKCGDLFRAHRDEIDGIIVTLPNFGDERGIADSIRYSKLDVPV
                     LVQATPDRRDDMKISDRRDSFCGKMSACNNLMQYGIKYSITAKHTVSPSSAEFAKDLD
                     WFTKVCRVTNGLRKLRIGAIGARPQAFNTVRYSEKLLERYGISVVTLDLSDIFGAIGR
                     MKDDEPEVRTKLAAIQAYVPTNGIPAAALMKMTKLGVAIERFMKDAHCTVSAVQCWTS
                     LEENFGVVPCTVMSMMSESLLPAACETDVMGTVSMHALALASQTPSALLDWNNNYGDD
                     ENKCVCFHCSNLPKHFFADVVMDFQAIIAGTVGKENTYGTCVGRVKAGPMSYVRFSTD
                     DYKGKIRGYIGEGEFTNDPLETFGGAGVAKIPNLQKLLHEICEQGFEHHVAANFSNVA
                     APVYEAAIRYLGWEMHWHDRNS"
BASE COUNT          660 a         1012 c         1034 g          680 t
ORIGIN      
        1 gcggcctgtc cgcggtgtgc ttcgccttca tgaccgtgct gatgagcggc atcaacatgt
       61 actccatggc cctggtcatg aaggtggtgc tcggctggga tctgaacttc tccatctggg
      121 tctcctccat caccgtcgcg gtctatgtgg cgctgggcgg cttgcgctcc gcgattttca
      181 acgagattct gcagttcctg ctcatctggc tcggcgcgct gctcatcccc atcctgggcc
      241 tggttgaagc cggtggctgg gagcccctga agcaccagat tgccgcgcgc atgggcagca
      301 tgcagtacac ccatctgtgg agcaccaccg cccacttccg cgacaacccc atgggcatcc
      361 actggacggg tctggtgttt gggctgggca ttgccatcag ctgcggctac tggaccacag
      421 atttcctggt tgtgcagaga gtcctgtcgg cgcacaacct gcgctcggcc cgcatggccc
      481 cgatcatcgg tgccggattc aagatgatgg tgccattcat cgtcatcctg cctgggttgc
      541 tggcgctgtc cgtactgccc ttcaagctgg tcccgcagga catcgcggtc gcgaccggcc
      601 agcacagcta caacgaggtg ctgccgctga tgctggtgcg ctactgcggt ccgggattgc
      661 tgggcctcgg cgtgaccgcg ctgattgccg gcttcatgtc cggcatggcc ggcaacgtga
      721 gcgctttcgc gaccgtctgg acctacgaca tctacaaggc cttcatgaac aagaaggcca
      781 ccgacagcca ttacgtatcg atggggcgct ggtgcacctt catcggcgtg ctggtatccg
      841 ttggcacggc atatctggtg cagcacgcgg ccagcattat ggattacgtg caggccctgt
      901 tcagcttctt catcgcccca ttgctcggca cggtcttgct gggcatgctg tggaagcgag
      961 cgacaaaggc tgccgggttc tggggactgt tggccggaac ctgcaccgcg atcgggctgt
     1021 tcctctgggt ccacttcaat cccgcggcgc tgcgctacgt cgcgctgtcg gccgatgcca
     1081 aggtgatggc cgaggatatg taccgggcgc tctggtcctg cctggtgtgc gtgatcgtga
     1141 ccgtgatcgt aagcctgatg acccagccga agacggacgc cgaactggaa ggcctggtct
     1201 acggcgcgtc gaagattccc gaggaacacg acgagcactg gtatcagcgg ccgctggtgt
     1261 gggctagcgt agtggcggtt gggttcatcg ttttaaacat cattttctgg tagggagctg
     1321 atgatgaagg gacatattca gatctggttc ttctctggga tcctgctgct catctacggg
     1381 gtcctcatca cggcgaccgg tttgtgggag ctcagtcacc ccctggccaa cccgcccgtg
     1441 ctgaacggtc tgcacgcgcc catctggtgg ggcgcgctca tggtagtcgc tggacttttc
     1501 tatacgttga aattccgtcc gcgctgaagc gggcggccgt attgcgttaa cgatctttga
     1561 ggaatgcatt catgaaaaag caattgacgt tcggagtcat tgttggcaac cgtgggtttt
     1621 tcccggatca tctggccaaa gagggccgcg aagaaatcct gaaagtgctg gacaaggccg
     1681 gcatcagagc catcaccctc gcaccggagc agtcgaagtt cggttcggtg gaaacgcatg
     1741 acgacgcgat taaatgcggc gacctgttcc gcgcgcatcg cgacgaaatt gacggcatta
     1801 tcgtgaccct gcccaacttc ggcgacgagc gcggcattgc cgattcgatt cgctactcga
     1861 agctggacgt tccggtgctg gtccaggcca cccccgatcg ccgcgacgac atgaaaatca
     1921 gcgaccgccg cgacagcttc tgcggcaaaa tgtcggcctg caacaacctc atgcagtacg
     1981 ggatcaagta ctcgatcacc gcgaagcaca ccgtgtcccc cagctcagcg gagttcgcaa
     2041 aggacctgga ctggttcacg aaggtctgcc gcgtgacgaa cggactgaga aagctgcgca
     2101 tcggcgccat tggcgcgcgg ccgcaggcct tcaataccgt gcgctacagc gagaagctac
     2161 tggagcggta cggaatctcc gtggtgacgc tggatctgtc ggacatcttc ggcgcgatcg
     2221 gccgcatgaa ggacgacgag cctgaagtca ggacgaagct ggccgcaatt caggcctatg
     2281 tccccacgaa tggcatcccg gcggcggcgc tcatgaagat gacgaagctc ggcgtcgcca
     2341 tcgagcgctt catgaaggac gcgcactgca ccgtcagtgc cgtccagtgc tggacctcgc
     2401 tcgaagagaa cttcggcgtc gtcccctgca cggtcatgag catgatgagc gaaagcctgc
     2461 tgcccgccgc gtgcgaaacc gacgtcatgg gcaccgtcag catgcacgcc ctcgccctcg
     2521 ccagccagac cccgagcgct ctgctcgact ggaacaacaa ctacggtgac gacgagaaca
     2581 agtgcgtctg cttccactgc tcgaatttgc cgaagcactt cttcgcggat gtcgtcatgg
     2641 acttccaggc catcatcgcc ggcaccgtcg gcaaggaaaa cacctatgga acctgcgtag
     2701 gccgtgtgaa agccggaccg atgagctacg tacgcttctc caccgacgac tacaagggca
     2761 agattcgcgg ctacatcggc gagggcgaat tcacgaacga tccgctggag acctttggcg
     2821 gcgcgggcgt ggcgaagatt ccaaacctgc agaaactgtt gcacgagatt tgcgagcagg
     2881 gcttcgagca tcacgtggcc gcaaacttct cgaatgtggc ggcgccggtc tacgaggcgg
     2941 ccatccggta cctcggctgg gagatgcact ggcacgacag aaacagctag ggaccaggga
     3001 atagtgaagc ggccggacga gagtccggcc gctttcttgc ttctcgagaa cgagcctcgc
     3061 atgccgcagc ttctgagata atcttcgttt ctgggcaagg gtgaagagtc aatacgagtg
     3121 tggtgtttct gaagttcctt taccttgtca tcccgaccgg accctgagcg tagcgaaggg
     3181 gaagcggagg gacctgcttc tcttccgcag aaagaaattt cggaaacacc acactcggtc
     3241 ctcagcaagc tctgagcgtt gtcgatctga tttcgatcgc tgtcatcgac atgcaccgga
     3301 aacgcccaat tgcttcgcgc tggaaggacg aacgagatgc ggaaagggtt cacacgagaa
     3361 ccgcaaagag acgaccccgc gacgcg
//
LOCUS       DASZYN010000696         2245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1194287, whole genome shotgun sequence.
ACCESSION   DASZYN010000696 DASZYN010000000
VERSION     DASZYN010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194287"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /locus_tag="VHE33_18035"
     CDS             <1..1284
                     /locus_tag="VHE33_18035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99408.1"
                     /translation="VPKGFTQNSPWQFSPRVGVTWNPDGSGKTVFRLGGALIYDEPNF
                     FTAQRNQQNPPFATAASTVPTTTPLNFADPWSSGSSPGNPFPQPQIPPKNVPFPATAQ
                     YIFMPHQFKPPYVMQWTASVQRELGHSWMAQLDYIGNETAHMTWGFPLNPAVYTSGSP
                     TANYSKRFRMVLANPTEGGKFTGGGGGTVLVADSATASYNGMIATIQHRTANYTVLAN
                     YTWSHCIDVEDAQGDFAATTVEDPYKPRLDRANCGFDYRGMFNANAVVISRFTSLHGV
                     AGAIVNNWELGPLMRVTDGDPFTVTEGDVNGDDVTNDRPNVTGKLYSDNEIANNKQYL
                     NTISFHLSADGTYGSMGRNALRGPGYFNLDAEISRFFPITERTKLDFRIEAFNMLNHP
                     NFNMPSQRAITSSTFGQITSAHDPRIFQGAVKFIF"
     gene            complement(1408..2202)
                     /locus_tag="VHE33_18040"
     CDS             complement(1408..2202)
                     /locus_tag="VHE33_18040"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVT99409.1"
                     /translation="MQPTPLHYILLSFAAGLGGLVNSVAGGGGFLLFPSLIKAGLLPI
                     QANATNTVSLWPGQFTSIAGYREDLRKNHRLVLPVVLVAFTGGLLGALTLLKGTQNSF
                     LRLVPYLFFLGATTFALSGPISRMIRRRAAEQAEHPGQLHRVAVVPLLALLLVITYYI
                     GYFGAGAGLLTMTALALCGVERIAEINALKVVITTVANAVASVTFIIGGAVEWRYCWL
                     LMIACAIGGYAGARNARRISDRVLRPIIIVLGFSIAIYFFVHRKGL"
BASE COUNT          510 a          672 c          670 g          393 t
ORIGIN      
        1 gtgcccaagg gcttcacgca gaactcaccc tggcagtttt cgccccgcgt cggagtgacc
       61 tggaaccccg atggttccgg caagacggtc ttccgccttg gcggtgcgct tatttatgac
      121 gagcctaatt tctttacggc tcagcggaac cagcagaatc cgcccttcgc aaccgcagcc
      181 tcgactgtcc cgacaacaac gccactcaac tttgccgatc cgtggtccag cggcagctcc
      241 ccaggtaatc ccttccctca acctcagatt cccccgaaga atgtgccctt tccggcgacc
      301 gctcagtaca tcttcatgcc gcatcagttc aagccgccct acgtaatgca gtggacggcg
      361 agcgtgcagc gcgaattggg ccactcctgg atggcgcagc tggactacat cggcaatgag
      421 accgcgcaca tgacatgggg attccccctc aatcccgcgg tctataccag cggctcgccg
      481 acggcgaact acagcaagcg gttccgtatg gttctggcga atcccaccga aggcggcaag
      541 ttcactggtg gcggaggagg aacggtcctg gttgccgact cagcaacggc cagctacaac
      601 ggcatgatcg ccaccatcca gcaccgcaca gcaaattaca cggtcctcgc caactacacg
      661 tggtcccact gcatcgacgt ggaggacgcg cagggtgatt tcgccgcaac cacagtggag
      721 gatccctaca aaccgcgcct cgaccgtgcc aactgcggct tcgattaccg cggcatgttc
      781 aatgcgaacg cggtggtgat cagccgcttc accagcctgc atggcgtcgc aggtgcgatc
      841 gtcaacaact gggaactggg gccactgatg agagtcacgg atggcgaccc cttcactgtc
      901 actgaaggag atgtgaacgg cgacgacgtc accaacgatc gccccaacgt taccgggaaa
      961 ctctactcgg ataacgaaat cgccaacaac aagcagtacc tcaacaccat ctcattccat
     1021 ttgtcggcag acggcaccta cggcagcatg ggacgaaacg ctctgcgcgg gccggggtac
     1081 ttcaacctgg atgcggagat cagtcgcttt ttcccgatca ccgagagaac gaagctggat
     1141 ttccggattg aagcattcaa tatgctgaac catccaaact tcaacatgcc ctcgcagcgt
     1201 gccattacat cgagcacttt cggccagatc acaagtgccc atgatcctcg catcttccag
     1261 ggcgctgtga agttcatctt ctagaccaat cttgaagcag cctcccaggc cgccattgac
     1321 tcaatggcgg cctctttgtt tgttagaacc agcattatgg gctgactgag cggcgcagga
     1381 tcagaccgcg gccgcattcg acgatgctca taaacccttg cggtggacga agaagtaaat
     1441 ggcgatgcta aagccgagaa caataatgat gggccggaga acgcggtcgg agatgcgccg
     1501 ggcattgcgg gcgccggcgt acccgccgat ggcgcaggcg atcatgagga gccagcagta
     1561 acgccactcc actgccccac caatgatgaa tgtgaccgaa gcgacggcgt tcgccacggt
     1621 agtgatgacg accttgagtg cgttgatttc ggcgatccgc tcgacaccgc acagcgcaag
     1681 agcggtcatc gtgagcaggc cggcgccggc tccgaaatag ccgatgtagt aggtgatgac
     1741 gagcagcagc gccaggaggg ggacaactgc gacgcgatgt aactgtccgg gatgctcggc
     1801 ctgctcagcg gcgcggcgtc ggatcatacg cgagatgggt ccgctcaggg caaaggtggt
     1861 ggctccgaga aagaacagat acggaaccag acgcaggaag gaattctgcg tgcccttgag
     1921 gagcgtcagc gctcccagca gaccaccggt gaacgcgaca aggaccaccg ggaggacgag
     1981 gcggtggttc ttgcggagat cctcacggta gccggcaatc gacgtgaact ggcccggcca
     2041 tagagaaacc gtgttggtgg cgttggcctg gatggggagc agtccggctt tgatgagtga
     2101 gggaaagaga aggaagccgc ctccgccagc gacggagttg accagcccgc cgaggccggc
     2161 cgcgaaagag agcaggatgt agtgaagggg cgtaggctgc actggctaaa ttgtatcgag
     2221 aggctgcagg cggtaggcag tagca
//
LOCUS       DASZYN010000697         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1007410, whole genome shotgun sequence.
ACCESSION   DASZYN010000697 DASZYN010000000
VERSION     DASZYN010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007410"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..441)
                     /locus_tag="VHE33_18045"
     CDS             complement(109..441)
                     /locus_tag="VHE33_18045"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVT99410.1"
                     /translation="MPVLFDRGTEPPVIRLEGEVDISQAAELKRVLLEALQAKGGMAI
                     AMETATSMDVTAVQLLWAAEREARRAGVSLALRGAVPEALCATAREAGLERFPLSGEA
                     GASAEVRA"
     gene            complement(461..823)
                     /locus_tag="VHE33_18050"
     CDS             complement(461..823)
                     /locus_tag="VHE33_18050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT99411.1"
                     /translation="MKQVLTVDDSASVRQMVSFTLRNAGYGVTEAADGRQGLEKARAE
                     KFDLVITDLNMPEMDGIEMIGGMRKLPAYAFTPILMLTTESQPEKKDAGRKAGATGWI
                     VKPFQAEQLIAVVQKLVR"
     gene            complement(960..>2735)
                     /locus_tag="VHE33_18055"
     CDS             complement(960..>2735)
                     /locus_tag="VHE33_18055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99412.1"
                     /translation="LPAGKECALGVGKRARPGVGRSMMPAAEGEAVVTVLSRVAGELR
                     TLLIDSEKEMEGLGHDFQALAREANAILEAAGVIVSCAESERMASVLPGVQKLACAAQ
                     EFIQGRLEATAGVLDTVIAEEKLLERLTQLTRGQKAIVKETGMLRVLTNIEVARLGEV
                     GAGFEYLAHELDDFSQAVARSTSELMEHTEGRRKATGETRRALANGLPGMREQFARIG
                     TRLESAVGEVDAALGELRQTPQRFRSCVEEVAAQIAGVVAAIQSHDITRQQIDHVEIA
                     LRIIAEGAEQDSATARAGLTIQNYQLRNVRRTIDGWTAQIRSCLEGIGHIASAELLDL
                     GRAVMRQEGTLSAQLTHIDRLEEECESSDAQVQASFAGIAGLMQLVTEHLERSKSVRD
                     RLQLLMFNSIVEASHLGTQADGILEISTSIKRISATWAGITTQSEEATGQIRGLVEAS
                     HGTVESFSEASYAGLREARGETMTGLEVLREAASCAERKGAEVEDAVRKVQARIAEIG
                     ATGERLEAGFRRLEAALEEIESVRRQMEEEGRGEGSFDRGAVEQRFAENYTTEMERAV
                     LRAALEGGPLPPVEETLAGNSVELF"
BASE COUNT          413 a         1123 c          687 g          512 t
ORIGIN      
        1 tccccggctg ttcgttcagt tccagcagca ccgattccag ttcgaccagc agttccctgg
       61 cctcctcgcg gaatccatgc cgaaactgat ccacgttgtt gggggccgtc acgctcgcac
      121 ctcagcggaa gccccggctt caccgctcag cgggaagcgc tccaaacccg cctcacgcgc
      181 cgtcgcacag agcgcctcgg gcaccgcgcc ccgcagagcc agagaaacgc ccgccctccg
      241 cgcttcacgc tcagccgccc acagcagctg caccgccgtg acatccatgc tggtcgccgt
      301 ctccattgcg atcgccatcc cgcctttcgc ctggagcgct tccagcagaa cccgcttcag
      361 ttccgcggcc tgagagatgt ccacctctcc ctccagccgg atcaccggcg gctctgttcc
      421 tcgatcgaat aacaccggca ccgcttcaac ctccagcccg ctagcgaacc agcttctgca
      481 ccacggcaat gagctgctcg gcctggaacg gtttcacaat ccatcccgtc gctcccgcct
      541 tgcggcccgc atccttcttc tccggctggg attcggtcgt cagcatcagg atcggcgtga
      601 acgcgtacgc gggcagcttg cgcatccccc cgatcatctc gatcccgtcc atctccggca
      661 tgttcagatc cgtaatcacc aggtcgaact tctccgcgcg ggctttttcg aggccctgcc
      721 gcccatcggc agcttcggtc accccgtatc ccgcgttgcg cagcgtgaag ctcaccatct
      781 gccgcacgct cgccgaatcg tctactgtca gaacctgctt catgcgaccc tatatccccc
      841 tcatcggaga aaaccagggg tttctccaga attgaaagtc ctcagtttgc tgttttcccg
      901 gaatggattc cgattcccaa aacggtcccg cgatgcccct ccgtccctcg ccgcaatctt
      961 cagaaaagtt ccacgctgtt gccggccagg gtctcttcca ccggcggcaa cggtcctcct
     1021 tcgagcgcgg cgcgcagcac ggctcgctcc atctccgtcg tatagttctc cgcgaagcgc
     1081 tgctccaccg cgccgcgatc gaaactcccc tcgccccggc cttcttcttc catctgccgc
     1141 cgcaccgatt cgatctcctc cagcgccgcc tccagccgcc ggaagcccgc ctccagccgt
     1201 tccccggtcg ccccgatctc ggcaatccgc gcctgaacct tgcggaccgc atcctcgacc
     1261 tcagcgccct tcctctccgc gcagctggct gcttcgcgca gaacctcaag gcccgtcatg
     1321 gtctcgccgc gcgcctcgcg cagccccgcg tagctcgcct cggaaaacga ctcgaccgtc
     1381 ccgtggcttg cctcgaccag cccccggatc tgccccgtcg cctcttccga ctgcgtcgtg
     1441 atcccggccc aggtcgccga gatccgcttg atgctggtcg aaatctccag aatcccgtcc
     1501 gcctgcgtgc ccagatggct cgcctcgacg atggagttga acatcagcag ctgcagccgg
     1561 tcgcgcaccg atttcgagcg ctccagatgc tcggtcacca gctgcatcag ccccgctatc
     1621 ccggcaaacg acgcctgtac ctgcgcgtcg ctcgattcgc actcctcctc cagccggtcg
     1681 atgtgggtca gctgcgccga gagcgttccc tcctgccgca tcaccgcccg ccccaggtcc
     1741 agaagctccg cggacgcgat gtgcccgatc ccctccagac aactgcgaat ctgcgccgtc
     1801 catccatcga tcgtgcgccg cacgttgcgc agctgatagt tctggatcgt cagccccgcc
     1861 cgcgcggtcg cgctgtcctg ctccgcgcct tcggcgataa tcctcagcgc gatttcgaca
     1921 tgatcgatct gctgccgggt aatgtcatgg ctctgaatgg cagccaccac gcccgcgatc
     1981 tgcgcggcca cctcttccac gcagctccgg aagcgttgcg gcgtctgccg gagctctccc
     2041 agcgccgcat cgacctcgcc gaccgcgctt tcgagcctcg ttccaatccg cgcaaactgc
     2101 tcccgcatcc ccggcagccc attggccagc gcccgccgcg tctcccccgt tgccttgcgc
     2161 cggccctcgg tgtgctccat cagttcgctg gtcgagcgcg cgaccgcctg cgagaagtca
     2221 tccagctcgt gcgccagata ctcaaatccc gcacccacct cgcccagccg cgccacctcg
     2281 atgttcgtca gcacccgcag catgccggtt tccttcacga tggccttctg cccccgcgtc
     2341 agctgcgtga gccgctccag cagcttctct tcggcgatca ccgtgtccag aacccccgcg
     2401 gtcgcctcca gccggccctg aatgaactcc tgcgccgcgc aggccagctt ctgcaccccc
     2461 ggcagcaccg acgccatgcg ctcgctctcg gcgcagctca cgatcacccc cgccgcttcg
     2521 aggatcgcgt tcgcctcccg cgccagcgcc tgaaaatcgt ggcccagacc ctccatctct
     2581 ttttccgaat cgatcagcag cgtgcgcagc tcccccgcaa cccggctcag gaccgtgacc
     2641 accgcttctc cttccgcagc cggcatcatg ctgcgaccca cgcccggccg tgcccttttg
     2701 ccaacaccca gcgcacactc cttccccgcc ggcaa
//
LOCUS       DASZYN010000698         7822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_296516, whole genome shotgun sequence.
ACCESSION   DASZYN010000698 DASZYN010000000
VERSION     DASZYN010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7822
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296516"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1440)
                     /locus_tag="VHE33_18060"
     CDS             complement(<1..1440)
                     /locus_tag="VHE33_18060"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HVT99413.1"
                     /translation="MRRRSVFIRRNRPHHSPARLIVFSTAFALVLAASAGLNARAQDT
                     TPPPPPPDQSAAPPQAPGQTQIRAVRISDVEGKVQVFEGEEVAFDQAEPNMPAVEGMR
                     LVTADNGRFEIEFEDGSVARVTPDSSIRLTQLRRNADGSTVTQIDALSGLSYYELNGR
                     GGEYSVHFGPNSATPVKSAVFRVSLDASPSTVAVMHGAVHIDDGMGMSMDIHPSQTFQ
                     TDLKQPGQFTVAQSVAADTWDQWNSDRDESLAKLETNQSIARASSGNPDNPAWNDLDY
                     YGNWYNLPGYGQAWAPSGVSANWDPFGVGAWGYYQGVGYTWISGYPWGWWPYHCGAWN
                     FQNGFGWMWVPGNCGSGFYGSGWYPYSTVWSVPPGYVPPLRPNGGIPINGPRLRPINP
                     RVPRLITVNRGPENSAPFRHPEGIRTPRNVEFQGKTIHPIEPGIHPIQRGPLGESFTN
                     AVIRTHPEMGNPAGRPMSGLMTGGRGSFNP"
     gene            complement(1708..2403)
                     /locus_tag="VHE33_18065"
     CDS             complement(1708..2403)
                     /locus_tag="VHE33_18065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99414.1"
                     /translation="MRFLSVSSLPVLALLAAAALTSAQTPPTLGSAHIVFSFEHPQLQ
                     PSQFTISIDETGAGRFESKPGPVSDPTDGVFPTPLDRPILLDDTLRADLFRYARAHNF
                     FATRCTTAKTSLAFTGKKTLSYSGPDGSGSCTFVWAEDPALQRLADDLGAVAYTLEEG
                     RRLALEVRHDRLSLDAELASLQDALKDHRASDLSNIAEQLHAIADNDQVMDRARKRAQ
                     ALLARSENPPKRD"
     gene            complement(2406..2918)
                     /gene="ruvC"
                     /locus_tag="VHE33_18070"
     CDS             complement(2406..2918)
                     /gene="ruvC"
                     /locus_tag="VHE33_18070"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HVT99415.1"
                     /translation="MRVCGIDCGTETTGYGVVEWDDRAREPRLVSISSGGICPPKKSQ
                     LPVRLAFIYAELTSLFERYSPDMVAVEEVFYSVNAKSAIKLGHVRGVALLAAATAGLP
                     VAEYAPLTIKSTVTGYGLAAKQQVQFMVARLLSLPAAPEPADAADALAIAICHIHHAQ
                     TLSRQEAHAL"
     gene            3107..3754
                     /locus_tag="VHE33_18075"
     CDS             3107..3754
                     /locus_tag="VHE33_18075"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HVT99416.1"
                     /translation="MKVETRERSLRERQKESTHGEIVDTAMEMILKTGDFSHEAIAGA
                     TGMAARTVYRHFPSREELIAATWDKLRARTGTRFPRTEEEILELAPEVYGNFDQNEKL
                     VRAFLFSGVGSEVRDRGAREGRPAFRAALKDLVGGLPVRKQKQVVAVFLALYSAPAWQ
                     MMRDRGELSGEDAGQAVRWALSALLASLKKDSILSTKGNRNGKSGNSPDSTAGRR"
     gene            3708..4211
                     /locus_tag="VHE33_18080"
     CDS             3708..4211
                     /locus_tag="VHE33_18080"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVT99417.1"
                     /translation="MASPAILQTPRLVAADRAAGQDILGIVVTTLVSNQETRDQFGVF
                     EALIPPGAGIPLHRHPDVELFYILEGQLCVVREVNGAAEEFVVARDQGGFIPADANHG
                     FVNNGVAPARALITCTRGLEAFLAEAGRPAKESAGGPPTGEEVERVLAIARKHGQVFP
                     GMASSGD"
     gene            complement(4240..5019)
                     /locus_tag="VHE33_18085"
     CDS             complement(4240..5019)
                     /locus_tag="VHE33_18085"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HVT99418.1"
                     /translation="MEPLVIAGKAFQSRLIVGTGKYRNGPETQAAIEASGAEMVTVAV
                     RRVNLDRTQESLLDFIDPRRYFLLPNTAGCYTADEAIRAARLAREVGLSDWVKIEVIG
                     DQATLYPDVQATLEATRTLVKEGFTVLPYTSDDIVFAHRLIDAGAAAVMPLAAPIGSG
                     LGIQNRATLQILRERITTVPLIVDAGVGTASDAALAMEMGADAVLMNTAIAAAANPIL
                     MAEAMQHAVLAGREAYLAGRMPRKLYATASSPLEGVSKVSA"
     gene            complement(5458..6240)
                     /locus_tag="VHE33_18090"
     CDS             complement(5458..6240)
                     /locus_tag="VHE33_18090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVT99419.1"
                     /translation="MNQPDFLFMRDVSVARGDNVVLHHISLRIANGEHVAILGPNGCG
                     KSTLIKTITCQCYPIVTPTTELRLLGRERWDVSQLRTHLGVVSAELPGERTPVTSGLD
                     AVVAGFFSASTLWPNLHVTPEMRDRAHQALDLMQATHLESKLVGEMSAGEQRRIMIAR
                     ALVHQPEMLLLDEPSNALDLAAQRELRQALRRVAQQGVGILMVTHHLADVFPEIARVI
                     MMRNGRIFADAPKTELLNPSKLRDLFGVEVTLVEHDGSWHSW"
     gene            6298..7545
                     /gene="argJ"
                     /locus_tag="VHE33_18095"
     CDS             6298..7545
                     /gene="argJ"
                     /locus_tag="VHE33_18095"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HVT99420.1"
                     /translation="MKMTSLIPSEALPRGFQFAAVKAGIKPSGKPDFAVAVADRIASA
                     AAMFTANRVQAAPIVVGRRHMERSGGLLRVVAVNAGNANCATGAAGMKAAEQVCAAAA
                     KTFGCDGHEVFPSSTGIIGVPLPAEKLLAALPAVKKALAANADGFSQFAEAILTTDTR
                     AKVAHAQINAGGKTVRIAGVCKGAGMIHPRLVSASAPGGSAPHATLLVYLFTDAAVHP
                     TALDRYVRAAVDASFNRISIDGDTSTNDTVLLMASGASGADVWAEDADFAGALTEVCT
                     SLAKQVVSDGEGVGHVVELQIEGAPSDADALTVAKSIAHSMLVKTAWAGSDPNWGRLM
                     AAVGYSGVAVDAEKIAIWFGELPICVNGGRAAEFDEQAAHKYLEQREFSIRIALGVGE
                     GRCRFWTSDLTAEYVRINAEYST"
BASE COUNT         1446 a         1962 c         2981 g         1433 t
ORIGIN      
        1 cgggttgaag cttcctcggc caccggtcat gagtccggac atgggccggc cggcgggatt
       61 gcccatctca ggatgcgtac gaatcacggc gttggtgaag ctttccccca gggggccgcg
      121 ctggatggga tggattccgg gttcgatggg atgaatcgtc ttcccctgga actcgacgtt
      181 gcgaggcgtt ctgattcctt ccggatggcg gaagggcgca gagttttccg ggccacgatt
      241 gacggtgatg agccgcggca cgcgcggatt gatggggcgc agcctcggtc cgttgatggg
      301 gataccaccg tttggacgaa gaggcgggac gtagccggga ggaacgctcc acacggtgga
      361 atacgggtac cagccggaac catagaagcc agagccgcag ttgccgggga cccacatcca
      421 gccgaagccg ttctggaaat tccaggcgcc gcagtggtag ggccaccagc cccagggata
      481 gccggagatc caggtgtatc cgacaccctg gtagtagccc catgcgccga cgccgaaggg
      541 atcccagttg gcgctgacac cggagggagc ccaggcctgg ccatagccgg gcaggttgta
      601 ccagttgccg tagtagtcga gatcgttcca cgccgggtta tcggggttgc cggagctggc
      661 gcgggcgatc gactgattgg tttcgagctt cgccagggac tcatcgcggt cagagttcca
      721 ctgatcccag gtgtcggcgg cgacgctttg cgcgactgtg aactggccgg gctgtttgag
      781 atcggtctgg aaggtctggc tgggatggat gtccatggac atgcccatgc cgtcatcaat
      841 atgaacggca ccgtgcatga cggcgacggt ggagggcgat gcgtcgagac tgacacggaa
      901 gacggcgctc ttgacgggtg tggcgctgtt ggggccgaag tgaacactgt attcgccgcc
      961 gcggccgttt agttcgtagt agctgaggcc gctgagcgcg tcgatttggg tcacggtgct
     1021 gccgtcggca ttgcggcgga gctgggtgag gcgaatggaa ctgtcggggg tgacacgggc
     1081 gacgctgcca tcctcaaatt caatttcgaa gcgcccgttg tccgcggtga cgaggcgcat
     1141 gccttcgacg gcgggcatat tgggctcggc ctggtcgaaa gcgacctcct cgccctcgaa
     1201 gacctggacc ttgccctcga catcgctgat gcggacggcg cgaatctggg tctggccggg
     1261 agcctgaggc ggcgcggcag actggtcggg tggaggcggc ggcggggtcg tgtcctgggc
     1321 gcgtgcgttc aaacctgcgg atgcggcaag cacgagagcg aaggcagtgg agaagacgat
     1381 caggcgagcc ggggagtgat gtggacggtt acggcgaatg aacacagagc ggcgcctcat
     1441 agtggcacct ccttcgagcg tcgacccttt tcactggtga ggcgggaggt caaactgcac
     1501 cgccggtcaa gctggcgcgc cggaatccct gccaccctgg gcctgaaggg aagacgctgt
     1561 ggcccacaac ccatctgcac tctgataacg agcgaatggg gtctaaagtt tcgacttatt
     1621 ctcgcacggt aagggaagcc tggggaatgg gaattcggtg gtacgcaaga attccggggg
     1681 gtcgatccca atctggaaat gccgcgatta gtcgcgtttg ggcgggtttt cactccgggc
     1741 aaggagcgcc tgagcgcgct tgcgggcccg gtccatgacc tggtcgttat cggcgatggc
     1801 gtggagctgc tcggcgatat tgctcaggtc tgaggcgcga tggtccttca gggcatcctg
     1861 gaggctggcg agttcggcat cgaggctgag gcggtcgtgg cggacttcga gcgcgagacg
     1921 gcggccttct tcgagggtat aagcaacggc gcccaggtca tcggcgaggc gctggagggc
     1981 gggatcctcc gcccagacga atgtgcagga accactgccg tcaggaccgg agtaggagag
     2041 ggttttcttg ccggtgaagg ccagggaggt cttcgcagtg gtgcatcggg tggcgaagaa
     2101 gttatgggcg cgggcgtaac gaaagagatc ggcgcggagt gtgtcgtcga ggaggatagg
     2161 gcgatcgagc ggggtgggga agacgccgtc ggtgggatcg gagacgggtc cgggcttcga
     2221 ttcgaagcgg ccagcgccgg tttcgtcgat ggagatggtg aactgcgagg gctggagctg
     2281 gggatgctcg aaggagaaga cgatgtgagc ggagccgagg gtgggaggcg tttgagccga
     2341 ggttagagcc gcggcggcga ggagagccag aacgggaaga gatgagacgg agaggaaccg
     2401 catggctaga gagcgtgcgc ctcctggcgg gagagggtct gggcgtggtg gatgtggcag
     2461 atggcgatgg cgagcgcatc ggctgcgtcg gcgggctcag gtgctgcggg cagagagagc
     2521 aggcgggcga ccatgaactg gacttgctgt ttagcagcga ggccgtagcc ggtgacggtg
     2581 gacttgatgg tcagcggggc atattcggca acgggaaggc cggcggtggc ggcggccagc
     2641 agcgcgacgc cgcgaacgtg gccgagtttg atggccgatt tcgcattgac ggagtagaag
     2701 acctcttcga cggcgaccat gtcggggctg tagcgctcga agagagaggt gagctcggcg
     2761 tagatgaagg caagacggac gggcaactga gattttttcg gagggcagat gccgccggag
     2821 gagatggaga cgaggcgtgg ttcccgagcg cgatcgtccc actcaacgac gccgtagccg
     2881 gtagtttcag ttccgcagtc gatgccgcag acgcgcatgg ggcctcagag gggcgatgtt
     2941 tcttgccgtg ggaaaagcca gggtcagttt agcgcaggtg acagcggggt tgaagctgac
     3001 gcagatggcg gaagagggca agctgcgtat gcggtccgct cgaagatgga gagaatcgat
     3061 ccggatgcag attgacagaa gtatgccgtt atgtcagtat tatgacatga aagttgaaac
     3121 ccgagaacgg agcctgcggg agcgacagaa ggaatcgacg catggggaga tcgtggatac
     3181 ggcgatggag atgatcctga agacgggaga cttcagccat gaagcgattg cgggcgcgac
     3241 gggcatggcg gcgcggacgg tataccggca ctttccgtct cgcgaggaac tgattgcggc
     3301 gacctgggac aagctgcggg cgaggacggg gacgagattt cctcgaacag aggaggaaat
     3361 tctggaattg gcgcccgagg tctacgggaa tttcgatcaa aacgagaagc tggtacgggc
     3421 gttcctgttt tccggcgtgg gctccgaggt gcgcgatcgc ggggcgcgcg aagggcggcc
     3481 agcgtttcgc gccgcgctga aagatctggt tggagggctg ccggtcagaa aacagaagca
     3541 ggtagtggct gtttttctgg cactttacag tgcgccggca tggcagatga tgcgggatcg
     3601 cggcgaactg agcggcgaag atgcaggaca ggccgtgcgc tgggcgttat cggcgttgct
     3661 cgcgagcctg aagaaggatt cgattctgtc gacgaaagga aatcggaatg gcaagtccgg
     3721 caattctcca gactccacgg ctggtcgccg ctgatcgggc ggccggccag gacattcttg
     3781 gcattgtggt cacaaccctg gtttcgaacc aggagacgcg cgatcaattc ggggtgttcg
     3841 aggcgctcat tcctcccggt gcgggcatcc cgctgcatcg ccatcctgat gtcgagcttt
     3901 tctacatcct ggaagggcag ctgtgtgttg tgcgggaggt gaacggcgcg gcggaggaat
     3961 ttgttgttgc acgggatcag ggcgggttca tcccggccga tgcgaatcat gggttcgtga
     4021 acaacggggt ggcgccggcg cgggcactga tcacctgcac gcgcggactg gaagcttttc
     4081 tggcggaggc gggccggccg gcaaaggaat ctgcgggtgg gccgccgacg ggcgaggaag
     4141 tggagcgggt gctggcgatc gcgcggaaac atgggcaagt gtttccggga atggcttcgt
     4201 cgggcgattg aatggcgcgc gatgccaggt gcgctgacct caggcgctga ccttcgagac
     4261 gccttcgagc ggcgagctgg cggtggcgta gagtttgcgc ggcatgcgcc cggcaagata
     4321 cgcctcgcgt ccggcgagaa cggcgtgctg catggcctct gccataagaa tggggttggc
     4381 cgcggcggcg atggcggtat tcatcagcac ggcgtcggcg cccatttcca tggcgagggc
     4441 tgcatcggag gcggtgccga ctccggcgtc gacgatgagg ggcacggtgg tgattctttc
     4501 gcgcaggatt tgcagggtcg cgcggttctg aatgccgagg ccggagccga tgggtgcggc
     4561 gaggggcatg acggcggcgg cgccggcgtc gatgaggcga tgcgcgaaga cgatgtcgtc
     4621 ggaggtatag ggcaggacgg tgaatccttc tttgacaagg gtgcgggtgg cctcgagggt
     4681 ggcctggacg tcgggataga gggtggcctg atcgccgatg acttcgatct tgacccagtc
     4741 ggagaggccg acttcacgag cgaggcgggc ggcgcggatg gcttcgtcgg cggtgtagca
     4801 gccggcggta ttcggaagca ggaagtagcg gcgcggatcg atgaagtcga gcagggattc
     4861 ctgagtacgg tcgagattga cgcggcgcac ggcaacggtg accatctcgg cgccggaggc
     4921 ttcgatcgcg gcctgggttt cgggtccgtt gcggtacttg ccggtgccga cgatcagacg
     4981 cgactggaag gctttgcctg cgataacgag gggttccatg gggacattgt agagcggtgg
     5041 gtggggtggg gcagcgacgg aaatcactgg accgtggggt ggggcggggc ggcgaaatgt
     5101 gaaaggcccg cagccagact ggctgcgggc cctgatcacc ggtgaactgt tgagattgac
     5161 ctgaggcgcc cccgctatcc ggagtagcct cggcggttgg atacgccagg acttattacc
     5221 ttggagccag gagcggctct aagcctcagt cacctcacgc aggttcctgc aactactacc
     5281 atcagaattt ttcattcgct ggatcatcct cctttcccgt gtacggataa gttaacccgt
     5341 ggactgcaat cgaggcaagc ttcgcctctc cgctgcatgt ctgacgccgt cggacattgt
     5401 cggacgcgtg tcggacactg gtgcagcatg cgatgaatac cgggcttccg tggaggctta
     5461 ccaggaatgc caggagccgt catgttccac gagggtgacc tcgacgccga acaggtcgcg
     5521 gagtttggag gggttgagca gttccgtttt gggagcgtcg gcaaagatcc ggccgttgcg
     5581 catcatgatg acgcgggcta tttcaggaaa gacgtcggcc aggtgatggg tgaccatgag
     5641 gatgccgacg ccctgctggg caacgcggcg cagagcctgg cgcagttcac gctgggcggc
     5701 aaggtcgagg gcgttggagg gctcgtcgag caggagcatt tccggctgat ggacgagagc
     5761 gcgggcgatc atgatgcggc gctgctcgcc ggccgacatt tcgccgacga gcttcgattc
     5821 gagatgggtg gcctgcatca agtcgagcgc ctggtgggcg cggtcgcgca tttccggtgt
     5881 gacatgcaga ttgggccaga gcgtcgaggc ggagaagaat ccggcgacga ccgcgtcgag
     5941 accgctggtg acgggggtgc gttcgccggg gagttctgcg gaaacgacgc cgagatgggt
     6001 gcggagctgg gagacgtccc agcgctcgcg accgaggagg cgcagctcgg tggtgggcgt
     6061 gacgatgggg tagcactggc aggtgatggt tttgatcagg gtggatttgc cacagccgtt
     6121 gggaccgagg atggcgacgt gttcgccgtt ggcgatgcgg agagagatat ggtgcaggac
     6181 gacgttgtcg ccgcgggcga cggagacgtc gcgcatgaag aggaagtcgg gttgattcat
     6241 cgtgagccgc taagagcgat tatggcaaag gcgtgagttg gtagattgga tgtgttcatg
     6301 aaaatgacgt ctctgattcc ttctgaagcg ctgccgcgtg gttttcagtt cgctgcggtg
     6361 aaggccggca ttaagccgag cgggaaaccg gatttcgcgg tggcggtggc ggatcggatt
     6421 gccagcgccg cggcgatgtt cacggcgaat cgcgtgcagg cagcgccgat cgtggtgggc
     6481 cgccggcata tggagcgcag cggtggcctg ctgcgggtgg ttgcggtgaa tgccggcaac
     6541 gcaaactgtg cgacgggggc cgccggaatg aaagcggccg agcaggtgtg cgctgcggcc
     6601 gcaaaaacct tcggttgtga tggacacgag gtttttcctt cttcaacggg aattatcggc
     6661 gtgccgctgc cggcggagaa actgctggcg gcactgccgg cggtgaaaaa agcactggcg
     6721 gccaacgcgg acgggttttc gcaattcgcc gaagcgattc tgacgacgga tacacgagcg
     6781 aaggtggcgc acgcgcagat caatgccggc ggcaagacag tgcgaattgc gggtgtgtgc
     6841 aaaggcgcgg ggatgatcca tccgcggctg gtgtcggcca gcgcgccggg aggctcagcg
     6901 ccgcatgcga ctctgctggt ttatctgttc accgacgcgg ccgtgcatcc gacggcgctc
     6961 gaccggtatg tgcgggccgc ggtggacgcg agcttcaatc gcatctcgat cgacggtgac
     7021 acttcgacga atgacacggt actgctgatg gcctcgggcg catcgggcgc cgatgtgtgg
     7081 gcggaagatg cggatttcgc cggggcactg accgaggtat gcacatcgct ggcgaagcag
     7141 gtggtgtccg atggcgaagg ggtcggtcat gttgtcgaat tgcagatcga gggtgcgccg
     7201 agcgatgcgg acgcgctgac ggtggccaag tcgatcgcgc actcgatgct ggtgaagacg
     7261 gcgtgggcgg gatcggatcc gaactgggga cggctgatgg cggcggtggg atattcgggt
     7321 gtggccgtcg atgcggagaa gatcgcgatt tggtttgggg agttgccgat ttgcgtcaac
     7381 ggcgggcgcg cggcggaatt cgatgagcag gcggcgcaca aatacctcga gcagcgggaa
     7441 ttttcgattc gcattgcgct tggagtgggt gaggggcgat gccgattctg gacgagcgat
     7501 ctgacggcgg agtacgtgcg gatcaatgcg gagtactcga cgtagaagca gcgttcggcg
     7561 tgcggcgttc cgcgttcagc gttcagaaaa gcaagcggca ggtcagcgcg cggagttgat
     7621 ccgagtcatg ttcgcaatgc gctcgcgcat gtgtgatgaa aatcacatcg cgtcataatc
     7681 gagaagtgca tcggagtgag tgcagaatgg tgaaggccgc atcgtttctg cggttgcgtg
     7741 cgcagaggcc cgggtatcca cagggttatt cacaaaactg ttgaaatctg tcagggaagc
     7801 ggatgtgcgc gtgcagcacg cg
//
LOCUS       DASZYN010000699         3728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1194696, whole genome shotgun sequence.
ACCESSION   DASZYN010000699 DASZYN010000000
VERSION     DASZYN010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194696"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VHE33_18100"
     CDS             complement(<1..575)
                     /locus_tag="VHE33_18100"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HVT99421.1"
                     /translation="MRLVLAAVSSNTQLSGVTRHAANLARCLMLYSRIEEVHVIAAPW
                     ECSMLRDAIGVEDPRLHIHCVHLDPHNLARNAWYYFDLPEIAAQLRADVVHFAYPAPL
                     NRRRMHCPVAVTVHDLYPWDIPENFGFPKVYFNRMILRQCIGAASALACVSDATLERL
                     REWEGAKIAAKAVRIYNSVEPMRRRAGRSPVP"
     gene            complement(572..1798)
                     /locus_tag="VHE33_18105"
     CDS             complement(572..1798)
                     /locus_tag="VHE33_18105"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HVT99422.1"
                     /translation="MPRENVLLLPGCAGFYLAAHLCITYLFFQAEPQTGAMVAFALQS
                     ALVAATAFYSFGPGHERLWAMVRIPPLRWVLGFLAVGLCSLSWSATVSLPVAVGYWIE
                     LAGQVVMIVLVLRAGPAEAVAGSVARGYVAGACLIALITWLSPTMEDLRPGNNDFFSP
                     NAIGFTCALGIYLLQYVSRREAEWRAGSGTVAVLLAITLLRTLSKTTIAAFAVGQVYL
                     LFFDRGMSRKSKVALGLASLVVIGAFWPLILHYTAVYENAGNQAETLTGRLGIWTFVL
                     ARAQEQPWIGHGFHSFRNVIPPFGSFEAWHAHNELLQQFYTYGVIGVVLLLGTYGSLH
                     RYAKRVAAPEVRVLVTSLLLFVVVRGLADTENFDLSLPLWLIALLSLTLGNAGCRTGE
                     KLRPETGRRALDGCAG"
     gene            complement(1822..3129)
                     /locus_tag="VHE33_18110"
     CDS             complement(1822..3129)
                     /locus_tag="VHE33_18110"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HVT99423.1"
                     /translation="MQTQLLEPATEVVKTTAEPRPANWVVNSTVLWEHRQLLARAIAI
                     SLAAGLLIAFLIPKKYTSTGRIMPPDNGGSSMALLAALTGGRGSTDMGGLGSLAGSLL
                     GMRNSSALFVDLLRSGTVSGDLIERFQLQHAWRKRYKVDAAKYLARHTTITDDKRSGV
                     ITVAVEDSDPRRARDLAQGYLEELNKLVTRTSTSSARQERIFIEKRLRGVEADLKQAQ
                     VALSEFASTHTTIDLSEQTRAMVDAGARLQAEQIAAQAEVDSLKQIYGDTNVRVRAAQ
                     ARAAEVQRQLTKLGGRAGDAEADATEEGGAAGELYPPLRQIPRLAVPYSELYRRVRVQ
                     EAVYQLLTQEYEVARIQEAKDVPAVSVIDAPGIPEKKSFPPRLLVAAVITVLSIGGTV
                     LFVLFRKRWELVEETDQRKVLARDVAETVRGHIRRVRAREGSS"
     gene            complement(3151..>3728)
                     /locus_tag="VHE33_18115"
     CDS             complement(3151..>3728)
                     /locus_tag="VHE33_18115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99424.1"
                     /translation="IRQIETSSAAARLSPNIESGSQGATLQLIQTQEEQMLARLKSEP
                     ASGRLVIHISESIENWAGTPADIEVRKGDVLRIPKRPGFVLVSGQVYNATAITFAPNK
                     TAGWYLSHAGGATRAADRKEIFVIRANGSVVGRGSGGWFGGGVLDTKLEPGDVVVVPQ
                     KIIGASLFWHNLLTGAQVASQIAIAAAVANL"
BASE COUNT          729 a         1398 c          918 g          683 t
ORIGIN      
        1 ggaactggac tcctccccgc ccgcctgcgc atcggttcca cagagttata gattcgcacc
       61 gccttcgccg caattttcgc gccttcccac tcccgcagcc gttccagggt cgcgtccgac
      121 acgcaagcca gtgcactcgc cgctcctatg cactgccgca agatcatgcg gttgaaataa
      181 accttgggaa atccaaagtt ctccggaata tcccacggat agagatcatg cacagtgacc
      241 gcgacggggc agtgcatcct gcgtcggttt agcggcgcag gatacgcaaa atgcaccacg
      301 tccgcccgca gctgtgccgc gatctccggc agatcgaagt agtaccaggc gtttcgcgcc
      361 agattgtgcg ggtccaggtg cacgcagtga atgtgcagcc tggggtcttc aacaccgatg
      421 gcatcacgca gcatcgaaca ctcccacggc gccgcgatca catgcacctc ctcgattcgc
      481 gaatacagca tcagacatcg cgccagattc gcggcgtgcc gcgtgacgcc ggagagctgc
      541 gtattggaag agaccgccgc cagcaccagt ctcatccagc acacccatcc agcgctctgc
      601 gcccggtctc gggcctcaac ttttctccgg tcctgcatcc ggcgttcccc aacgtcagac
      661 tgaggagggc aataagccac agaggcagtg acaaatcaaa attttctgtg tccgccagtc
      721 cgcgtaccac aacaaagagc aacagactcg ttaccagcac gcgaacctcc ggcgccgcca
      781 ctcgctttgc ataccgatgc aggctgccgt aggtgcccag cagcagcaca acgccgatca
      841 cgccatacgt gtaaaactgc tgcagcaact cgttgtgcgc gtgccacgct tcaaacgagc
      901 caaatggagg aatcacattt ctgaatgaat gaaacccgtg cccgatccac ggctgctcct
      961 gggctcgcgc taacacaaac gtccagattc ccagtcgccc tgtcagggtc tcggcctggt
     1021 tccccgcatt ttcatacacc gccgtgtagt gcaggatcag cggccagaac gcgccaatca
     1081 cgaccagact cgcgagcccc agcgcaacct tgctcttccg gctcatcccg cgatcgaaga
     1141 acagcagata gacctgcccc accgcaaacg ccgcaatcgt tgtcttgctg agcgtccgca
     1201 gcagcgtaat cgccagcagc actgccaccg ttcctgaccc cgcccgccac tcggcctccc
     1261 ggcggctcac gtactgcaac aagtagattc ccagggcaca ggtaaatcca attgcattcg
     1321 ggctgaagaa atcattgttc cctggccgca gatcctccat cgtcggcgat agccacgtaa
     1381 tcagcgcaat caggcacgcg cccgccacat atccacgcgc aacggatccc gcaaccgcct
     1441 ccgccggacc cgcccgcagc accagcacaa tcatgaccac ctggcccgcc agttcgatcc
     1501 aatacccaac cgctaccggc agcgacaccg tcgcgctcca gctcagactg cacaatccca
     1561 ccgccagaaa accgagaacc caccgcagcg gcggtattcg aaccatggcc cacagtcgct
     1621 catggcctgg tccaaacgaa tagaaagccg tcgccgccac cagcgccgat tgcagcgcaa
     1681 acgccaccat cgcgcccgtc tgtggctctg cctgaaaaaa cagatacgtg atgcacagat
     1741 gcgccgcgag atagaacccc gcgcatcccg gcagcagcag gacgttttct cgaggcacag
     1801 caatttccgc cgctctgacg gtcacgacga gccttcacgc gctcgcacgc gccgaatatg
     1861 gccgcgcacc gtttccgcca cgtcgcgcgc cagcactttt cgctgatccg tctcctccac
     1921 cagttcccaa cgcttgcgaa acagaacaaa cagcaccgtc ccaccgatcg acagcaccgt
     1981 gatcaccgcc gccaccagca gtcgtggcgg gaaggacttc ttctccggta ttccgggagc
     2041 gtcaatgacg ctcaccgccg gcacatcctt cgcttcctgg attcgcgcca cctcatattc
     2101 ctgcgtcagc aactggtaca ccgcctcctg cacgcgtacc cgtcgataca actccgagta
     2161 aggaaccgcg aggcgcggta tttgtcgcag tggtggatac aattcgccgg cagcccctcc
     2221 ctcttccgtc gcatcggcct cggcgtctcc ggccctaccg cccagcttcg tcaactgccg
     2281 ctgcacctcc gctgcccgcg cctgcgccgc acgtacccgc acattcgtgt cgccgtagat
     2341 ctgcttcaaa gaatcaactt ccgcctgagc cgcaatctgc tccgcctgca gccgcgcgcc
     2401 tgcgtccacc atcgcccgtg tctgttccga aagatcgatc gtcgtgtgcg tgctcgcgaa
     2461 ctcgctcaga gccacctgag cctgcttcag gtccgcctcc acgcctcgca atcgcttttc
     2521 gatgaaaatt ctctcctgcc gcgccgacga cgtgctcgtc cgcgttacca gcttgttcag
     2581 ttcttccaga tagccctgcg ccagatcccg cgcccgccgc ggatcgctgt cctccaccgc
     2641 caccgtgatc actccgctcc gcttgtcgtc cgtgatcgtc gtgtggcgtg ccagatactt
     2701 cgccgcatcc actttgtatc gtttgcgcca ggcatgttgc agttgaaacc gttcgatcag
     2761 gtcgccactc accgtgccgc tgcgcagcaa atcgacaaag agcgcgctgc tgttgcgcat
     2821 ccccagcaag ctgccggcca ggctccccag tccccccata tcggttgaac cgcgcccacc
     2881 ggtcagcgcc gccagcagcg ccatgctcga cccgccgtta tccggcggca tgatccgccc
     2941 cgtcgacgtg tacttcttcg gaatcagaaa cgcgatcagc aatcccgccg ccagcgaaat
     3001 cgcaattgca cgcgccagca gttgccgatg ctcccacagc accgtcgaat tcaccaccca
     3061 gttcgccggt cgcggctctg cggtcgtctt cacaacctcc gtcgctggct ccagcagttg
     3121 cgtctgcatc gcgctccccg aatcattcgc ttagaggttg gccaccgctg ccgcaatcgc
     3181 aatctgcgac gccacctgcg caccggtcag caggttgtgc caaaacagcg acgccccgat
     3241 aatcttctgc ggcaccacca ccacatcccc aggctccagc tttgtatcca gcaccccgcc
     3301 accgaaccat ccgcccgacc cgcgccccac caccgaacca ttcgcgcgga tcacgaagat
     3361 ttccttccgg tctgctgccc gtgtcgcccc gcctgcgtgg ctcagatacc accccgccgt
     3421 cttgttcggc gcaaacgtga tcgcggtcgc gttgtatacc tgtccgctca ccagcacgaa
     3481 cccaggccgc ttcggaatgc gcagcacgtc gcccttgcgc acctcaatat cggccggcgt
     3541 ccccgcccag ttctcaatgc tctcgctgat gtgaatcacc agccgtccac tcgctggttc
     3601 gctcttcagc cgcgccagca tctgctcttc ctgcgtctga atcaactgca gcgtcgctcc
     3661 ctgcgatccg ctttcgatgt tcggcgaaag gcgcgccgcc gcactgcttg tttcaatctg
     3721 ccgaatca
//
LOCUS       DASZYN010000700        13455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_493110, whole genome shotgun sequence.
ACCESSION   DASZYN010000700 DASZYN010000000
VERSION     DASZYN010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 13455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13455
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493110"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VHE33_18120"
     CDS             complement(<1..987)
                     /locus_tag="VHE33_18120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT99425.1"
                     /translation="MTPDDKVNILMVDDQPAKLLSYEVILADLGENLIKATSAKEALN
                     ILLKNDVAVVLMDVSMPELDGFELADLIREHPRFGKTAIIFISGVHLTDQDKINGYRR
                     GAVDYISVPVIPEVLRAKIGVFVELHRKTLLLQRLNTDLERLVAERTEELRQSEAHHR
                     ERAELLDLATEAIIVRDLNGSIRFWNAGAEALYGWSRQDVLGSDLHQLMRTTFPVSRS
                     ETEHALLTRRSWEGNLVVRARDGRELVVATRKVLNRERAAILEIHRDITAQIQAEEAL
                     RQTEKLAAMGRVAGIIAHEINNPLEAITNAFYLLRNHPSLDQEARYYANLAEQ"
     gene            complement(1018..7314)
                     /locus_tag="VHE33_18125"
     CDS             complement(1018..7314)
                     /locus_tag="VHE33_18125"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HVT99426.1"
                     /translation="MATAAQPGPKSKPSQKHLPGNGLDSSVPDYLPVILMGLQSMKDG
                     DFTVRLPGSWTGLAGKIADHFNEIVSANEQMASELKRVGQAVGKEGKTRERIRVPRHR
                     GAWDEMETSVNNLIEDLLRPTTEVTRAIASVAQGNLTQTVRLDVDGRPLEGEFLRSAT
                     IVNTMIQQLGIFAGEVTRVAREVGTEGKLGGQALVPGVAGTWKDLTDSVNSMASNLTG
                     QVRNIAEVTTAVASGDLSRKITVDVRGEILQLKEAINTMVDQLRSFASEVTRVAREVG
                     TDGKLGGQAVVPGVAGTWKDLTDSVNAMAGNLTAQVRNIAEVTTAVARGDLSRKITVD
                     VKGEILELKDTINTMVDQLNAFAGEVTRVAREVGTEGKLGGQASVPGVAGTWKDLTDN
                     VNFMASNLTGQVRNIAEVATAIANGDLSRKITVDVRGEILQLKETLNTMVDQLNRFAG
                     EVTRVAREVGTEGKLGGQANVPGVAGTWKDLTDNVNSMASNLTGQVRNIAEVTTAVAR
                     GDLSRKITVDVKGEILELKNTINTMVDQLNAFAGEVTRVAREVGTDGRLGGQAMVPGV
                     AGTWKDLTDSVNFMAGNLTAQVRNIAEVATAVARGDLSRKITVDVKGEILELKDTINT
                     MVDQLNAFAGEVTRVAREVGTDGKLGGQAEVPGVAGTWKDLTDNVNFMASNLTGQVRN
                     IAEVATAIASGDLSKKITVDVSGEILRLKETINTMVDQLNRFAGEVTRVAREVGTEGR
                     LGGQANVPGVAGTWKDLTDNVNSMASNLTGQVRNIAEVTTAVASGDLSRKITVDVKGE
                     ILELKNTINTMVDQLNAFAAEVTRVAREVGTDGKLGGQAEVPGVAGTWKDLTDSVNSM
                     ASNLTGQVRNIAEVATAIANGDLSRKITVDVRGEILQLKETLNTMVEQLRSFAAEVTR
                     VAREVGSEGRLGGQANVSGVAGTWKDLTDSVNAMAGNLTAQVRNIAEVTTAVARGDLS
                     RKIAVDVKGEILELKNTINTMVDQLNAFAGEVTRVGREVGTEGKLGGQANVPGVAGIW
                     KDLTDNVNVMASNLTEQVRGIVKVVTAVANGDLKQKLFVNAKGEVGALAETINNMTDT
                     LATFADQVTTEAREVGVEGRLGGQANVPGAAGTWKDLTGNVNLLADNLTNQVRAIAEV
                     ATAVTRGDLTRSIQVEARGEVADLKDNINTMIDNLRVTTDRNTEQDWLKTNLARFTGM
                     LQGQRDLATVGRMLLSELAPLVNAQQGVIYQTESQTETEETGGLVLLSAFGNTQDGFS
                     KQVGIGEGLIGQCAVEKRRMLITEVPGTAVPIRSGLFEAIPRNVIVLPVLFEDRVKAV
                     IELASLSTFTASHMAFLDQLTSSIGIVLNSIEATMQTEALLKQSQQLAGELQTQQKEL
                     QQTNEQLAQKAQQLAEQNSEVERKNQEIEQARRALEEKARELALTSKYKSEFLANMSH
                     ELRTPLNSILVLGQQLAENPDGNLTGRQVEFARTIHGAGTDLLNLISDILDLSKIESG
                     TVSVDPEELFFGSLLEAVSRPFRHEAENRKLFFEVKSDPKLGRSLVTDPRRLQQVLKN
                     LLSNAFKFTERGGVRLTVSSALSGWSPDHPILSGAGAVVAFEVSDTGIGIPAEKQRII
                     FEAFQQADAGTSRKYGGTGLGLAISRELASLLGGEIQLRSTPSKGSTFTLYVPQTYVG
                     PAAPPVAEKPRPSAPSYLAKPVVSEQDSGIEDDRENLQPGDNVLLVVEDDPHYARVIA
                     DLSHDKGFKVLCAMSGAEALSLAREYNPSAISLDVFLPDMLGWTVLNHLKADPRTRHI
                     PVQMLTLDDDRRHGLSRGAFSFVTKPTSTEGLEAALTRIHDFSRPRRKRLLVIEDDPA
                     EQLSIRELLGYKDIDIDVADSGAAALETLETSGYDCAVLDLRLPDMSGFEVLDRLRES
                     DKLRDLPVVVFTGRELTPDEDARLHMLARSVVVKDVESPERLLDETALFLHRVVADLP
                     AEKQRMLDRLHQSDEALHGRKVLIVDDDVRNIFALSSVLERRGMTVLTAGTGREAIAT
                     IESTSDLAIVLMDIMMPEMDGYETMQVIRQNASLRRLPIIALTAKAMKGDREKCLEAG
                     ASEYLAKPVNTEQLLSALRMWLHR"
     gene            7617..8681
                     /locus_tag="VHE33_18130"
     CDS             7617..8681
                     /locus_tag="VHE33_18130"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HVT99427.1"
                     /translation="MLFAGTQTVNGSGSKGIYAYRWDPVGGDLQPAGLAAESENPTFL
                     AVAPNGEFLFAANELAQFEGQASGAVTAFAIDRAGAKLKRINQVLAQGTGTCHVAVDH
                     QGRVAFCANYNGGSATSFVINSTGQISDAVSHYQYEGHGPNKDRQEAPHAHRVTPSPD
                     DRFLFVNDLGLDCIHIYRLDDASARLTPNDPPQWNATPGSGPRALRFHPNGKLAYCVH
                     EMASQVEVLDWDRGKGTFNSLQKVSLIPEDYHGATRGSDIVITRDGHFAYAANRDYDC
                     LVSFAVDPKSGKLTMLARGSCGGKIPRHLTLDPTEKFVLVANEQSDKISVFARDEKTG
                     KLGETAKDFVIEKPQCLVWA"
     gene            complement(8758..10686)
                     /locus_tag="VHE33_18135"
     CDS             complement(8758..10686)
                     /locus_tag="VHE33_18135"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HVT99428.1"
                     /translation="MGRRIIAASLAVLLVPFVPIGVLAQSGYPAQPQPAYPSPPPQYD
                     SPQTPQAYPQAQQDYPPPPPDYAQPQSDPSYPPPPQYQQAPDPYSQPPQYQQSGPDEA
                     YAQAYDDTYQPYAAPQQPQSALSPEQLNQLIAPIALYPDQLIAQILAAATYPMQIAEA
                     DRWRQSMGNAPPDEIAAGANAQPWDPSVKALAAFPQVLDDLNRNLSWTTELGNAYYNQ
                     PQDVFSAIQVMRQRAQSAGNLQSTPQQEVSSDQGYIDIAPVNPQVVYVPSYNPWAVYG
                     APVDPYPGFDVLGAIGDFGVRFGPGLVMSAFTSMPWGFFGWGLSWLGNCLFFDHGGYY
                     PHGPGLRDWGLRYGGPRAPWGRNFAYARAYDRGFNRGFNRGYDRGGWNRGGYSARGGY
                     GNRGGYNARGRDYARGGFDGRYGYNNRGYAGNRGFQNGGFGNRGLAPGRGFADPRTGP
                     ATRPGYGYGNGFSRGSQQAWNRQPSFNGRSYGGYGDYGRTPGSSSPAFRSAPGYSRPS
                     VPSGLSRGFGNSFRQSQPTFRSAPTYRAYNGGSSRFENRGFANRGFSQPRSFNGGSHS
                     FFGGGRSESAPHFSNRSFGGGGHSFGGSNHSFSGGGHSFGGGGHSFGGGGHSFGGGGH
                     SGGGHFGGGHGGGGGHRH"
     gene            complement(11058..12005)
                     /locus_tag="VHE33_18140"
     CDS             complement(11058..12005)
                     /locus_tag="VHE33_18140"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HVT99429.1"
                     /translation="MFALIALVVIGIFVLITIVKTMYTVRTYTAGIVERFGRFNRVAQ
                     PGLHILVPWCETVRFVDLQIRQADVNVETKTKDNVFVTIPVSVQYQVLPDKVFEAYYK
                     LSSPQKQIESYVFNSILGHVPTLTLDEAFEQQATISAAVKRELDAVMSEFGYNILKAL
                     VTDIVPDAKVKAAMNDINAAQREQTAAQARGEAEKILKVKQAEAEAQSKALQGQGVAQ
                     QRQAIIQGLQASVEQFQSAVEGSTARDVMSMVLLTQYFDTLRDIGTTGRSNTILLPNQ
                     PGVVNDLLTQIMAGFSASTTGFDGNGTTPRQAPNPTATR"
     gene            complement(12188..12793)
                     /locus_tag="VHE33_18145"
     CDS             complement(12188..12793)
                     /locus_tag="VHE33_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99430.1"
                     /translation="MKSSLLLGLLFLLLPGARVSSASAPNALRNRISHDTVIQGYPCA
                     RGETWFYPDGSLNQCRLSHSATLGDLRVPGGSLVEFWSNGAAHYLMLQRPAVLAGHKV
                     RGGTRWGLSRGATTTFYGTGELRSFYLVRNESVQNVPCRGGSWNTYTDPTGGQTVVEL
                     YRDGSLASCKLSRDYAGFRSGQRIILPHLTVEADNKSIPAQ"
     gene            complement(13273..>13455)
                     /locus_tag="VHE33_18150"
     CDS             complement(13273..>13455)
                     /locus_tag="VHE33_18150"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HVT99431.1"
                     /translation="IGICGEHGGEPESVKFCHRAGLDYVSCSPYRLLTARLAAAQAAI
                     EEQKIFNPDLRTGNSK"
BASE COUNT         2400 a         3544 c         4845 g         2666 t
ORIGIN      
        1 ctgttcggcg aggttggcgt agtagcgggc ttcctgatcc agggaggggt gattgcgcag
       61 caggtagaag gcgttcgtga tggcctcgag ggggttgttg atttcgtggg cgatgatgcc
      121 ggcgacgcgg cccatggcgg cgagcttttc ggtctggcga agggcttcct cggcctgaat
      181 ctgagcggtg atgtcgcgat ggatttcgag aatggcggca cgttcgcgat tcaggacctt
      241 gcgggttgcg accacgagct cgcgtccgtc cctggctcgc accaccagat tgccttccca
      301 tgagcgcctg gtgagcagcg cgtgttcggt ttcggatcgg gaaacgggga aggtggtgcg
      361 catgagctgg tggaggtcgc tgccgagaac atcctggcgg ctccagccat agagggcttc
      421 ggcgccagcg ttccagaagc ggatggagcc gttcagatcc cgcacgatga tagcttctgt
      481 ggcgaggtcg agaagctcgg cacgttcgcg atggtgagct tcgctttgac gcagttcttc
      541 ggtgcgttca gcgacgagac gttcaaggtc agtattgagg cgctgcagca ggagggtctt
      601 gcggtggagc tcgacaaaga cgccgatttt ggcgcgcagg acttcgggaa tgacggggac
      661 cgagatgtag tcgacagcgc cgcggcggta gccgttgatc ttgtcctggt cggtgaggtg
      721 gacgcccgag ataaagatga tggcggtttt gccaaaccgc gggtgctcgc ggatcaagtc
      781 ggcgagctcg aagccgtcga gttcgggcat gctgacgtcc ataaggacga ccgcgacgtc
      841 gttcttgaga aggatattga gggcctcttt ggcggaggta gccttgatga ggttctcgcc
      901 caggtcggcg aggatcacct catagctgag gagcttggcg ggctggtcgt ccaccatgag
      961 gatgttgact ttgtcgtctg gagtcatcga tctctccggc cctgaatcca atcctttcta
     1021 ccgatgtagc cacatccgca gcgcggagag caactgttcg gtgttcaccg gcttggcgag
     1081 atattccgat gcgcccgctt cgagacattt ttcgcggtcg cctttcatgg ctttggcggt
     1141 aagagcaatg atgggcagcc gccgcagcga agcgttctga cgaatgacct gcatcgtttc
     1201 gtatccgtcc atctccggca tcatgatgtc cataagaacg atggcgaggt cggacgtgga
     1261 ttcgatggtg gcaatggctt cgcgtccggt tcccgcggtg aggacggtca tgccgcgacg
     1321 ctccagaacg ctggagagag cgaagatgtt gcggacgtcg tcgtcaacga tgaggacctt
     1381 gcgaccgtgg agggcctcgt cggattgatg caggcgatcg agcatgcgct gcttctccgc
     1441 cgggaggtcg gcgacgacgc ggtgcagaaa cagagcggtt tcatcgagga ggcgctcagg
     1501 tgattcgacg tccttcacga cgacgctgcg ggcgagcatg tgcaggcgag catcttcgtc
     1561 gggggtaagt tcgcggccgg taaaaacgac gaccgggagg tctcttaatt tgtcactttc
     1621 gcggagacga tcgaggactt cgaagccgct catatcgggc aggcgcaggt cgagcacagc
     1681 gcagtcgtag ccggaggttt ccagggtttc aagggccgcg gctccggaat cagcgacgtc
     1741 gatgtcgatg tccttgtagc cgagaagttc acgaatgctg agctgctcgg caggatcgtc
     1801 ttcaatgacc agcaggcgct tgcggcgcgg ccgggaaaaa tcatggatgc gagtcagggc
     1861 ggcttcgaga ccctcggtcg acgttggttt ggtgacgaag gagaaggctc ctcgggaaag
     1921 accgtgacgc cggtcgtcat cgagggtgag catctgcacc gggatgtggc gggtgcgggg
     1981 gtcggccttg aggtgattca ggacggtcca gccgagcatg tcgggcagga agacgtcgag
     2041 ggagatggcg gaggggttgt actcccgcgc gagagagagg gcctcggcac cgctcatcgc
     2101 acaaaggacc ttgaagccct tgtcatgcga aaggtcggcg atgacgcggg cgtagtgcgg
     2161 atcgtcttcg acaacgagca gaacgttgtc gccgggctgg agattttcgc gatcgtcctc
     2221 gatgccggag tcctgttcgg agacaaccgg tttggcgaga taggacggag ccgatgggcg
     2281 cggcttttca gcgacaggcg gggcggcagg gccgacgtac gtttgcggca cgtaaagagt
     2341 gaaggtgctg cccttgctgg gcgtactgcg gagctgaatt tcgccgccga ggaggctggc
     2401 gagctcgcgg ctgatggcga ggccaagccc agtgccgccg tacttgcggc tcgtgcctgc
     2461 atcggcctgc tggaacgcct cgaagatgat gcgttgcttt tcggctggaa ttccgatccc
     2521 ggtatcggag acttcgaagg ccaccacggc accggctccg gagaggatgg gatggtcagg
     2581 gctccagccg gagagagcgc tggagacggt caggcgaacg ccgccccgct cggtgaactt
     2641 gaaggcgttg gaaagcaggt tcttgaggac ctgctggagg cggcgtggat cggtgaccag
     2701 gctgcgaccg agtttgggat cgctcttgac ctcgaagaag agcttgcggt tttcggcctc
     2761 atgacggaac ggccgtgaga ccgcttcgag gagagagccg aagaaaagct cttcgggatc
     2821 gaccgaaacc gtgccggact cgatcttgga gaggtccagg atgtcggaga tgaggttgag
     2881 caggtcggtg ccggcgccgt gaatggtccg ggcgaattca acctggcggc cggtgaggtt
     2941 gccgtcggga ttctcagcga gttgctgacc aagaacgagg atggagttga gcggcgtacg
     3001 cagctcgtgg gacatgttgg ccaggaactc ggatttgtac ttggaggtga gggcgagttc
     3061 gcgtgccttt tcttcgagcg cgcgacgggc ctgctcgatt tcctggttct tgcgttcgac
     3121 ttcggagttc tgctcggcga gctgctgggc tttctgcgcg agctgttcgt tggtctgttg
     3181 caactccttt tgctgcgtct gcagttcgcc ggcaagctgt tgggactgct tcagcagagc
     3241 ctcggtctgc atcgtggcct cgatggagtt gagcacgatg ccaatggagg aggtgagctg
     3301 gtccaggaaa gccatgtggg acgcagtgaa cgtgctgagg ctggcgagtt cgatgacggc
     3361 tttgacgcgg tcttcgaaca ggaccggcag gacgatgacg ttgcgaggga tggcctcaaa
     3421 gagtcccgat cggatgggga cggcggtgcc cgggacttca gtgatcagca tgcggcgctt
     3481 ctccaccgcg cactgtccga tgaggccttc gccaattccg acttgtttgg agaagccatc
     3541 ctgggtgttg ccgaaggcgg agaggagcac gagaccgccg gtctcttcgg tttccgtctg
     3601 gctttcggtc tggtagatca ccccctgctg ggcgttgacc agaggagcga gctcggagag
     3661 gagcatgcgg cccacggtgg cgaggtcgcg ctggccctgg agcatgccgg tgaagcgagc
     3721 gaggttggtc ttgagccaat cctgctctgt attgcggtcg gtggtgaccc gcaggttgtc
     3781 gatcatcgtg ttgatgttgt ctttcaggtc ggcaacttcg ccgcgggcct cgacctgaat
     3841 ggaacgagtg aggtcgccgc gagtgacggc ggtggcgact tcggcgatgg cgcgaacctg
     3901 attggtgagg ttatccgcga gcaggttgac gttgccggtg agatccttcc aggttccggc
     3961 ggcgccgggg acgttggcct ggccgccgag acggccctca acgccgactt cgcgggcctc
     4021 cgtggtgacc tgatcggcga aggtggccag ggtgtccgtc atattgttga tggtttcggc
     4081 gagtgcaccg acctcgcctt tcgcattgac gaaaagcttc tgcttgaggt cgccgttagc
     4141 gacggcggta acaactttga cgatgccacg gacctgttct gtgaggttcg aggccatgac
     4201 gttgacgttg tcggtgaggt ccttccagat gccggcaact ccgggaacgt tggcctggcc
     4261 gccgagcttg ccttcggtgc cgacttcgcg gccgacgcgg gtgacttcac cggcgaaggc
     4321 gttgagctgg tcgaccatgg tgttgatcgt gttcttcagc tcaaggattt cgcctttgac
     4381 gtcgacggcg attttgcggg atagatcgcc gcgggcgacc gcggtggtga cttcggcgat
     4441 gttgcggacc tgagctgtga ggttgccggc catcgcgttg acagagtcgg tgagatcctt
     4501 ccaggtgccg gcgacgccgg aaacgttggc ctggccgccg agccggcctt cggagccgac
     4561 ttcgcgcgcc acgcgcgtca cttcggcagc aaaggagcgc agctgctcga ccatggtgtt
     4621 gagggtttct ttgagctgca ggatttcgcc gcggacgtcg acggtgatct tgcgggagag
     4681 atcgccgttg gcgatggcgg tggcgacttc ggcgatgttg cgcacctggc cggtgagatt
     4741 agaggccatg gagttgaccg aatcggtgag gtccttccag gtgccggcaa cgccgggcac
     4801 ttcggcctga ccgccgagtt tgccgtcagt gccgacttcg cgtgcgacac gcgtgacttc
     4861 ggctgcgaag gcgttgagct gatcgaccat ggtgttgatg gtgtttttga gctcgaggat
     4921 ctcgcctttg acgtcgacgg tgatcttgcg ggagaggtcg cctgaggcca cggcagtggt
     4981 gacttcagcg atgttgcgga cctggccagt gaggttggag gccatggagt tgacgttgtc
     5041 ggtaaggtcc ttccaggtgc cagcgacgcc gggcacgttg gcctgaccgc cgagccgccc
     5101 ctcggtgccg acttcgcgtg cgacgcgagt gacttcgccg gcgaaccggt tgagctggtc
     5161 caccatggtg ttaatggttt ctttcaggcg gagaatctcg ccggagacgt cgacagtaat
     5221 tttcttggag aggtcgccgg aggcgatggc ggtagcgact tcggcgatgt tgcggacctg
     5281 gccggtgagg ttcgaggcca tgaagttcac gttatcggtg aggtccttcc aggttccggc
     5341 aacgcccggt acttcggcct gaccgccgag cttgccgtcg gtgccgactt cgcgggcgac
     5401 gcgggtgact tcgccggcga aggcgttgag ctggtcgacc atggtgttaa tggtgtcttt
     5461 gagctcgagg atttcgcctt tgacgtcgac ggtgatcttg cgggagaggt cgccgcgagc
     5521 gacggcggtg gcgacttcgg cgatgttgcg gacctgggca gtgaggttgc cggccatgaa
     5581 gttcaccgag tcggtgagat ctttccatgt tccggcgacg ccgggcacca tggcctgacc
     5641 gccgaggcga ccgtcggtgc cgacttcgcg agcgacacgg gtgacttcgc cggcgaaggc
     5701 gttgagctgg tcgaccatcg tattgatggt gtttttgagc tccaggattt cgcccttcac
     5761 gtcgacggtg atcttgcggg agaggtcgcc gcgcgcgacg gcagtggtga cttcagcaat
     5821 gttgcggacc tgaccggtga ggttggaggc catggaattg acgttgtcgg tgaggtcctt
     5881 ccatgttccg gcgacgccgg gcacgttggc ctggccgccc agcttgccct cggtgccgac
     5941 ctcgcgggcg acgcgcgtaa cttcgccggc gaagcggttg agctggtcga ccatggtgtt
     6001 gagggtttct ttgagctgga ggatttcgcc gcgcacgtcg acagtgattt tgcgggaaag
     6061 atcgccgttg gcgatggcgg tagcgacctc agcgatgttg cggacctggc cggtgaggtt
     6121 ggaggccatg aagttgacgt tgtcggtgag atccttccat gttccggcga caccgggcac
     6181 actggcctgg ccgccgagct tgccttcggt accgacttcg cgggcaacgc gggtgacttc
     6241 gccggcgaag gcgttgagct ggtcaaccat ggtgttgatg gtgtctttga gctcgaggat
     6301 ttcgcccttc acgtcgacgg tgatcttgcg agagaggtcg ccacgggcga cggcggtggt
     6361 gacttcggca atgttacgga cctgggcggt aaggttgccg gccatggcgt tgacggagtc
     6421 ggtgagatcc ttccaggtgc cggcgacgcc ggggacgacg gcctggccgc caagtttgcc
     6481 gtcggtgccg acttcgcggg cgacgcgggt gacctcagag gcgaaggagc ggagctgatc
     6541 gaccatcgtg ttgatggctt ctttgagctg gagaatctca ccgcggacgt cgacggtgat
     6601 tttgcgggag aggtcgccgg aggcgacggc ggttgtgact tcggcgatgt tgcggacctg
     6661 gccggtgagg ttcgaggcca tggagttgac ggagtcggtg agatctttcc aggtgccggc
     6721 gacgccgggg accagagcct ggccgccgag tttgccttcg gtgccgactt cgcgggcgac
     6781 gcgcgtgact tcgccggcga agatgccgag ctgctggatc atggtgttga cgatggtcgc
     6841 ggaacgcagg aattcgcctt caaggggacg gccgtcgacg tcgaggcgaa ccgtttgggt
     6901 gagattgccc tgcgcgacgg aagcgatggc gcgcgtgacc tcggtggtgg gacgaaggag
     6961 atcttcgatg aggttgttga ccgaggtttc catctcgtcc caggcgccgc ggtggcgggg
     7021 gacgcggatg cgctcacggg tcttgccctc tttgccgacg gcctggccca cgcgtttcag
     7081 ttcgctggcc atctgctcat tggcggagac aatctcattg aagtggtcgg cgatcttgcc
     7141 ggcgagtccg gtccaggagc cgggtaggcg gaccgtgaag tcaccgtcct tcatgctttg
     7201 caggcccatg agaatgacgg gaaggtagtc cggcaccgag gaatcgaggc cattgccagg
     7261 cagatgtttt tgggagggtt tcgatttggg accgggctga gccgctgtcg ccatggaaga
     7321 ctcctgatgt ggagctgact atcaccccgg gatcgaggcc ggaaaaggca cacacccggg
     7381 ccctatgcct gaagggagcg ttgggtggat gcccaaaaat cgataaatgg ttgcctcagg
     7441 atggcggatg aattttcctg gtgctgtccg ctgctcagtg gtttcgaccg gggtcaatga
     7501 gcgatgatgg accagcgatg agacagctta gcagaaggac gttcgtgcgg tgggcggcgg
     7561 cagcgccgct tgcgggccgg gtactggggt tacacacagg ggaggagaaa agacggatgc
     7621 tgtttgcagg aacgcagacg gtgaacggca gcggcagcaa ggggatctac gcttaccgtt
     7681 gggatccggt gggaggcgac ttgcagcccg ccggattggc agcggagagt gagaacccga
     7741 cgtttcttgc tgtcgcaccc aacggggagt ttttgtttgc ggccaatgag cttgcgcagt
     7801 tcgaggggca ggccagcggc gcggtgacgg cgtttgcgat cgatcgcgcg ggagcgaagc
     7861 tgaagcggat caaccaggtt ctggcgcagg ggacggggac ctgccatgtc gcggtggatc
     7921 accaggggcg tgtggcgttt tgcgcgaact ataacggcgg gagcgcgacc tccttcgtga
     7981 tcaactccac cgggcaaatc agcgatgcgg tgtcgcacta ccagtacgag gggcacgggc
     8041 ccaacaagga tcgtcaggag gcgccgcacg cacatcgcgt gacgccgtcg ccggacgacc
     8101 ggtttttgtt tgtgaacgat ctgggtctgg actgcattca catctatcgc ctggatgatg
     8161 ccagcgcgcg gctcacgccg aacgatccgc cgcagtggaa tgcgacgccg ggatcggggc
     8221 cgcgggcatt gcggtttcat cccaacggaa agcttgccta ttgcgtgcac gagatggctt
     8281 cgcaggtgga ggtgctggac tgggatcgag ggaagggaac attcaactcg ctacagaagg
     8341 taagcctgat tcccgaggac tatcacggag cgacgcgcgg ttcggacatc gtgatcacgc
     8401 gcgacggaca cttcgcgtat gcggcgaacc gggactatga ctgcctggtc agttttgcgg
     8461 tcgatccgaa aagcggcaag ctgacgatgc tggcgcgcgg atcgtgcggc gggaaaattc
     8521 cgcgccatct gacgctggat ccgacggaga agtttgtgct ggtggcgaac gagcagtcgg
     8581 ataaaatctc ggtgtttgcg cgcgacgaaa agaccgggaa gctgggggag accgcgaagg
     8641 actttgtgat cgagaagccg cagtgtctgg tgtgggcgtg aatcagggat ccgggatgtg
     8701 aaaaagggca tggcatgaag ccgtgccctt tttgaaacgc agaggtgtcg gaagggttta
     8761 gtggcgatgt ccgccgccgc ctccgtgccc gccaccaaag tggccgccac cggaatgtcc
     8821 tccaccgcca aagctgtgcc ccccgcctcc gaagctgtgg cctccgcctc cgaaagaatg
     8881 accgccaccg ctgaagctgt ggttgcttcc accgaaggag tgtcctccgc cgccgaaact
     8941 gcggttgctg aaatgcggcg cgctctcgga gcgacctccg ccgaagaagc tgtggctgcc
     9001 gccattgaag gagcggggct ggctaaagcc gcggttggcg aagccacgat tctcaaacct
     9061 cgacgagccg ccgttgtagg cgcggtaggt gggcgcggaa cggaacgtgg gctgcgattg
     9121 tcggaacgag ttgccgaagc cgcggctcag accgcttggc acggacgggc gactgtaccc
     9181 gggagcggag cggaaggccg gtgaagaaga gccgggtgtg cggccgtaat cgccatatcc
     9241 accataacta cggccattga aactgggttg ccgattccag gcttgttgtg agcctcggct
     9301 gaacccgttg ccgtagccat atccgggtcg ggtggcgggt ccggtgcgcg gatcggcgaa
     9361 tcctcggccg ggagccaacc cgcggttgcc gaagccgccg ttctggaatc cgcgattgcc
     9421 ggcgtatcca cgattgttgt agccgtagcg gccgtcgaac ccgccccgtg catagtcgcg
     9481 gccgcgcgcg ttatatccgc cgcggtttcc atatccaccg cgggcgctat agcccccgcg
     9541 gttccagccg ccacgatcgt agccgcgatt gaagccgcgg ttgaatccac ggtcgtaggc
     9601 acgagcatag gcgaagttcc tgccccatgg agcgcgcgga ccgccgtagc gcagtcccca
     9661 gtcgcggagg ccaggcccat gcgggtagta gccgccgtga tcgaagaaga ggcagttgcc
     9721 gagccagctc agtccccatc cgaagaagcc ccagggcatc gaggtgaatg cggacatgac
     9781 gaggccgggg ccaaagcgga caccaaagtc gccgatggcg ccgaggacgt cgaagccggg
     9841 gtacggatcg accggagcac cgtagaccgc ccagggatta taggaaggga cgtaaacaac
     9901 ctggggattc acgggggcga tatcgatgta gccctgatcg ctgctgacct cctgctgggg
     9961 cgtcgattgc agattgccgg cgctctgggc gcgctgccgc atcacctgga tggcgctgaa
    10021 gacatcctgc ggctggttgt aatacgcatt gccaagttcg gtggtccagg aaaggttgcg
    10081 gttcaggtcg tcgagaacct ggggaaatgc ggcgagcgct ttgacgctgg gatcccacgg
    10141 ctgcgcgttg gctccggcgg caatttcgtc gggaggagca tttcccatcg attggcgcca
    10201 gcgatcggct tcggcgatct gcatcgggta ggtggcggcg gcgaggatct gggcgatgag
    10261 ctgatcgggg tagagggcga tgggagcgat gagctggttg agctgttcgg gcgagagcgc
    10321 ggattgtggc tgctgtgggg cagcgtaggg ctggtaggtg tcgtcgtacg cctgagcgta
    10381 tgcttcgtcg ggcccggatt gctgatattg cggaggctgc gaatacggat cgggcgcctg
    10441 ttgatattgc gggggcgggg gataggatgg atcgctttgc ggctgtgcgt agtcaggtgg
    10501 gggagggggg tagtcctgtt gtgcctgggg ataggcctga ggagtttgcg ggctgtcgta
    10561 ttggggtggt ggggaagggt aagccggttg gggctgtgcc gggtagccgg actgggccag
    10621 aacgccgatt ggcacaaatg gtacgaggag gacggccaga ctcgcggcta tgatccgacg
    10681 acccacgggg atccgggact cggggtttcc agaatcgggt tcgggttgct ggggggaaga
    10741 gaattcagga gcagcggaaa acgacggtcg atgattcata cgccctcaag gcctgcacag
    10801 ggtttgaagc cctgtgtttt cttgccgttt gcaggacggg aatcctgccg tgcagaacga
    10861 acaaactcgc cgaccccagg gccgggcgcg agcgaatgag cccgtggatg actggggtcc
    10921 catccttata acccgggggt gggggaatgg ttccgcgaga tttgtcgtta tttgaggaat
    10981 ggccgggact ggccgtccag gcaggcacgc tgcgcagtca gcgtgactga accatccggt
    11041 agacggccgt ctcccgctta acgcgttgct gttgggtttg gagcttgacg cggcgtggtc
    11101 ccattgccat cgaagccggt cgtgcttgcg ctgaagccgg ccataatctg ggtgagcaga
    11161 tcgttgacga cgccgggctg attgggcagc aggatggtgt tggagcggcc ggtggtgccg
    11221 atgtcgcgca gggtgtcgaa gtactgagtg agcaggacca tggacatgac gtcgcgcgcg
    11281 gtggaacctt cgacggcgga ctggaactgc tcgacggaag cctggagacc ctggatgatg
    11341 gcctggcgct gctgagcgac gccctgtccc tggagagctt tggattgcgc ttcggcttcg
    11401 gcctgcttga ctttgagaat cttctcggcc tcgccgcggg cctgggcggc ggtttgttcg
    11461 cgctgcgcgg cgttgatgtc gttcatcgcg gctttcactt tggcgtcggg gacaatgtcg
    11521 gtgacgagcg ccttgaggat gttgtagccg aactcgctca tgaccgcatc gagctcgcgc
    11581 ttgacggcgg ccgagatagt ggcctgctgc tcgaaggctt cgtcgagcgt gagcgtcggc
    11641 acgtgaccga ggatggagtt gaagacgtag gactcgatct gtttttgagg cgagctgagc
    11701 ttgtaataag cctcgaagac cttgtcgggc agaacctgat actggacgga aacaggaatg
    11761 gtgacaaaga cattgtcctt ggttttcgtc tcgacgttga cgtcggcctg acggatctgc
    11821 agatcgacga agcgaacggt ttcgcaccac ggcaccagga tgtggaggcc gggctgggcg
    11881 acacgattaa atctgccgaa acgttccacg atgcccgcag tgtaggtgcg gacggtgtac
    11941 atggtcttca cgatggtgat aaggacgaaa atgccaatga cgaccagggc gatcagggcg
    12001 aacatcgaag cggcttccac aggaagtcct ttctggtact gaaccttttg gagagagtcg
    12061 ggaacaatat aacgccgacc gggagaattg cgaacagaga gcgctcgacg agcgcgctct
    12121 ggagacggaa tcgggcacga agggttctcg cgagcggcgg accccggtgg ggtagcggag
    12181 cggcgtctta ctgggccggg atcgatttgt tgtcggcttc cacggtcaga tggggcaaga
    12241 tgatgcgctg accggaacga aatccggcat aatcgcggct gagtttgcag gatgcgagac
    12301 ttccgtcacg atagagctcg acgacagtct ggccgcccgt tggatccgtg taggtgttcc
    12361 acgagccccc gcggcagggg acgttctgaa cagactcgtt ccggaccagg taaaaggaac
    12421 gcagttcacc cgtgccatag aacgtcgtgg tggccccgcg ggagagtccc catcgcgtac
    12481 cgcctcggac cttatggccg gcaaggacag cagggcgctg gagcatgagg taatgggcag
    12541 cgccattgga ccaaaactca accagggagc cgcctggaac ccgcaggtcg ccgagggttg
    12601 cggaatgcga cagcctgcac tggttcaggg aaccatcagg atagaaccag gtctctcctc
    12661 tggcgcaggg atagccctgg attacggtgt cgtgcgaaat ccgattgcgg agcgcgtttg
    12721 gggcagaggc cgaggagacg cgggcgccag gcagcaggag aaaaaggagg ccgagcagaa
    12781 gactggattt catgggcaac tccctgacga ggacctccat acatcccttg ggtcccgtct
    12841 gaaccgggtg gtagccaatg aatcggctcc gaatgctgtg gtatgccggg gacacagaaa
    12901 ccggaaccga ttcagtgaag agggttggac agtagtgtgt gcctaatggg ccgggtgcgc
    12961 aagattacga aggtgccctg acgaaggggg tggatcactg cagtcacggg tgaggcaatg
    13021 caagaggaac gaggcggcaa tgggcgggaa gaagccccgt cgcgctcctc tgccaggaac
    13081 gcgacgggaa tgcctgcaag tgcgacaaag gaacacacat gcgacagaaa gggcgaatct
    13141 ccctggcaat agctgccagt agccggtagc cacaggctga tcggcagcgg acatgctggc
    13201 ttgtctgcat cccgttgtat gacagcaaaa agctttccca gggagcacaa ctggctccct
    13261 gggaaactcg agttacttcg aattgccggt ccgcagatcc gggttgaaga ttttctgctc
    13321 ttcgatggct gcctgcgcgg cggcgagacg cgcggtgagc agccggtaag gcgagcagga
    13381 gacgtagtcg agtccggcgc ggtggcagaa cttcaccgat tcgggctcgc cgccatgttc
    13441 gccgcagatg ccgat
//
LOCUS       DASZYN010000701         3197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1068520, whole genome shotgun sequence.
ACCESSION   DASZYN010000701 DASZYN010000000
VERSION     DASZYN010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068520"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VHE33_18155"
     CDS             complement(<1..395)
                     /locus_tag="VHE33_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99432.1"
                     /translation="MDLRRLLCARFRFAGIVTPILIAAPAALLRAESAPPADIVIRGA
                     TVMTASHGTIEHGAVWVHAGKIAGVGATVDAPASATVVDGTGLWLTPGIIDPHSHSAL
                     DSDVNEATSPVTPSMHMIDAFDNRDADLYR"
     gene            633..911
                     /locus_tag="VHE33_18160"
     CDS             633..911
                     /locus_tag="VHE33_18160"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HVT99433.1"
                     /translation="MKIMSTFTIRAGCVPEAAKRFLSGKVTPPAGVRILGRWHKTDCS
                     GGYTLFETDNPALLYEFAAAWTDLLENHSNVVIEDAEAGAALAKIYGQ"
     gene            1035..1739
                     /gene="pyrH"
                     /locus_tag="VHE33_18165"
     CDS             1035..1739
                     /gene="pyrH"
                     /locus_tag="VHE33_18165"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HVT99434.1"
                     /translation="MYKRVVLKLSGEALAAGKGFGVDPIRIHEIAGELAEVHQMGVEV
                     AIVVGGGNFFRGVAEQAREMDRVSADNMGMLATVINALAIQDALEKRDVYCRVMSAIE
                     MNQVCEPYIRRRAIRHLEKGRVVIFAAGTGNPYFSTDTAASLRAMEIKADVLLKATKV
                     EGIYDADPVISTDAVKFDQITYMDILRLGLKVMDTTAVSLCKDNNLPMIIFNLNQSGN
                     IRRVISGEKVGSLVTA"
     gene            1933..3042
                     /locus_tag="VHE33_18170"
     CDS             1933..3042
                     /locus_tag="VHE33_18170"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HVT99435.1"
                     /translation="MKTKKEHLPALSGLRAFSAMNIVFYHFCNPQWFGPLSPIVENGY
                     TSVSFFLLLSGFVLAYNYSDRAQKGQLKLKSFWIARISRLYPIYLVALVVSLGMLAEE
                     WHARPHAEFAWGVFLTPLMLQGWSPSLSTFWNTPAWTMCTEAFFYLIFPLVILWRRPK
                     HLGRLCAILLGLWVLGMVAPSLYMAFHPDGVLHPDRYTEGFWMRALKFTPPPHIPSFL
                     FGIVLADIDSLIPRGARARFVLGTLGLVGILTVLYFGNYLPFPMMHDGLMMPLFGMAV
                     LGLAGRNPMASFFGFVPFVAVGQASYCLYILHFNLWNLIHDHHFVARFGLSSYDPWLS
                     YALLVAIAVLAMKWIERPAQKWIRATWLPQPGKVT"
BASE COUNT          598 a         1007 c          934 g          658 t
ORIGIN      
        1 cgatacaaat ccgcatcgcg attgtcgaag gcgtcgatca tgtgcatcga cggcgtcacc
       61 gggctggtcg cctcattcac gtcgctgtcg agcgccgagt gcgagtgcgg atcgatgatg
      121 ccaggcgtaa gccagagacc ggtgccatcg acgacggtcg ccgaagcagg cgcgtcgacc
      181 gtggcgccaa ccccggcgat cttgccggcg tgtacccaca cggcgccgtg ttcgatcgtg
      241 ccgtggctgg cggtcatgac cgttgccccg cgaatgacga tgtcagccgg cggcgcggat
      301 tccgccctca gcagcgctgc gggtgcggca atcagtatcg gcgtaacaat cccggcgaaa
      361 cggaatcggg cgcaaagcag acggcgaaga tccatcaggt gacctccgaa ctgcacaaag
      421 gaatgtgggg actgcagcga ttgcacgctg gcccgtacag atctgtatgg ggacgatgaa
      481 gcttgccgcc atcgtacatg agaaaaggga attgggccca cgcccggaga acgaggccgc
      541 aacccgatgt tcacttctcg tgctagactt tgcgcctcgg ctcgtccccc acgaggttcc
      601 ctccgaagac ccatgaaaga aaggttcttc ccatgaaaat catgtcgacg tttaccattc
      661 gcgcaggctg cgttcccgag gctgcgaagc gcttcctgag cggcaaagtc acgcctcctg
      721 cgggcgtcag gatcctcggt cgctggcaca agaccgactg cagcggcggc tacacgctgt
      781 tcgagaccga taacccggct ctgctgtacg agttcgccgc agcctggacc gatctgctgg
      841 agaatcacag caacgtagtg atcgaggacg ccgaggccgg ggcagcgctg gcgaaaattt
      901 acgggcaata gttttgacgc aaaccgtatt ccccgccgtg ccggatttgg cctggcgggg
      961 attcgtttcc gcccaggaag aacgccccga tactcttcat gacgttggcg gcggcatgcg
     1021 atactgcctt tggcatgtac aaacgcgttg ttctcaagct ttcgggtgag gctctggccg
     1081 caggcaaggg cttcggcgtc gatcccattc gtatccacga aattgccgga gaattagccg
     1141 aagtccatca gatgggcgtc gaagtcgcca tcgtcgtcgg tggcggcaat ttcttccgcg
     1201 gcgttgccga acaggctcgc gagatggacc gcgtttccgc cgacaacatg ggcatgctcg
     1261 cgaccgtcat caacgccctc gccatccagg acgcccttga gaaacgcgac gtctactgcc
     1321 gcgtcatgtc ggccatcgag atgaatcagg tgtgcgagcc ctatatccgc cgccgcgcca
     1381 tccggcatct ggaaaaggga agggtcgtca tttttgccgc cggaaccggc aatccttact
     1441 tctccaccga cacggccgcc tccctccggg cgatggaaat caaggcagat gtgctactta
     1501 aggccactaa agtcgagggc atctacgacg ccgaccccgt catctcaacg gacgcagtca
     1561 aatttgacca gattacctac atggatatcc tgcgcttggg gcttaaggtg atggacacca
     1621 cagccgtgag tctgtgtaag gacaataatt tgcccatgat catcttcaat ctgaaccagt
     1681 cgggcaatat ccgccgggtc atctccgggg agaaggtcgg ctcgctggtc acagcctgaa
     1741 gcggtcctcc gaaccgcgca tggagccaac gttcgacccg agtttcgctg ctgagtggaa
     1801 ctcacgcctt ggagcgatgt cacgagagac tgaaggagaa gaccgcttct gagcgcacaa
     1861 gtccaggtcg tcccaggtcc catcctggaa caggctcccc caccgaaagc gccggccggc
     1921 aagccggaag tggtgaagac aaagaaggag catctgcccg cgctgagcgg actccgcgcg
     1981 ttttcggcga tgaacatcgt cttctaccac ttctgcaatc ctcagtggtt cgggccgctc
     2041 tcgcccattg tcgagaacgg ctacacctca gtcagctttt tcctgctgct ctcaggattt
     2101 gttctggcct acaactactc cgaccgggcg cagaagggcc agctcaagct gaagagcttc
     2161 tggattgccc gcatctcgcg cctctatccc atttacctcg tcgcgctggt ggtatccctc
     2221 ggcatgctcg cggaagagtg gcacgcacgc ccccacgccg agttcgcatg gggcgtcttt
     2281 ctgacgccgc tgatgctcca gggctggagc ccctcgcttt ccacgttctg gaacaccccg
     2341 gcgtggacca tgtgcaccga ggcgtttttc tatctgatct ttcccctcgt cattctgtgg
     2401 cggcgcccga agcacctcgg ccgcctgtgc gcaatcctgc tgggcctgtg ggtcctggga
     2461 atggtggcgc ccagtctcta tatggcgttc catcccgacg gcgtcctcca tccggaccgc
     2521 tataccgaag gattctggat gcgcgcgctg aaattcacgc cccctcccca catcccctcg
     2581 tttctcttcg ggattgtgct cgcagacatc gactccctga tcccacgcgg tgcgcgcgcc
     2641 cggttcgtgc tcggtaccct cgggttggtc ggaatcctga ccgtgctcta cttcgggaat
     2701 taccttccct ttccgatgat gcacgacgga ctgatgatgc ccctgtttgg catggctgtg
     2761 ctgggcctgg caggaaggaa tccgatggcg agcttcttcg gtttcgttcc ctttgtcgcc
     2821 gtcgggcagg ccagttattg tctctatatt ttgcacttta acctgtggaa cctcatccat
     2881 gaccaccact ttgtggcgcg cttcggcctg tccagctacg acccttggct gagctacgcc
     2941 ctgctggttg caatcgcggt gctggcaatg aaatggatcg agcgccctgc ccagaaatgg
     3001 atcagggcga cgtggctgcc gcaacccggg aaagtcactt agccgtcagc ggccgcacct
     3061 ccgccgccgt gcacaccacg gttcggtttc gccccgtatt cttggcctcg taaagcgcct
     3121 ggtccgcgag actcagaagt tccgtctctg tgcgggcgca ctgtggagca agagtggccc
     3181 cgatgctgat ggtcagg
//
LOCUS       DASZYN010000702         2061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_807638, whole genome shotgun sequence.
ACCESSION   DASZYN010000702 DASZYN010000000
VERSION     DASZYN010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807638"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1685
                     /gene="guaB"
                     /locus_tag="VHE33_18175"
     CDS             162..1685
                     /gene="guaB"
                     /locus_tag="VHE33_18175"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HVT99436.1"
                     /translation="MIEQPLPEALTFDDVLLTPSWSEIAPAQVSTATRLTKSIPLNTP
                     LLSAAMDTVTESRMAIAMAQQGGLGIVHRNLTIEQQAGEIDKVKRSESGMIVDPVTMA
                     PEQLISDALEVMRRYKISGVPVTRNKKLVGILTNRDLRFETRTDVPIGEVMTKENLIT
                     VPVGTTLEQAEEILHHHRVEKLLVVDEHYELKGLITVKDIQKKLKYPNASKDEQGRLR
                     VGAAIGATGDFLERAAELVRNRCDVLAIDSAHGHSSRVLDAVREVKKRFPHVDLLAGN
                     VATREGTEALIDAGADAVKVGIGPGSICTTRMVTGAGMPQITAISEAYKAAQPRGIPV
                     IADGGIKYSGDITKAVAAGASVIMIGSLFAGVDESPGETILYQGRSFKAYRGMGSLSA
                     MAQGGGERYFQGTKDLDLDSSNESVVNRERNGSSNRLGKFVPEGIEGRVPYRGALEAM
                     VYQLVGGLRSGMGYCGAATIEELQQKAKFVRISSAGLRESHVHDVVITREAPNYHVE"
BASE COUNT          410 a          779 c          529 g          343 t
ORIGIN      
        1 ccgaatccgg gcgtggacct tatccggatc agttcagcct tgccccgcgc gcataaaagg
       61 gcgctcccgg cccccggctg ccggctgttc tacaatgtcc caaagccgcc agtcaaaaga
      121 accactctga aacaatcgag gctgacgcgc atctttccat catgattgag cagcccctgc
      181 ccgaagccct tacatttgac gacgtcctcc ttaccccgtc ctggagcgaa atcgcgcccg
      241 cgcaagtctc cacagccacc cgcctgacga agagcatccc cctcaacaca cctctgctct
      301 ccgctgccat ggacaccgtc accgaatccc gcatggccat tgcgatggcg cagcagggcg
      361 gcctcggcat cgtccatcgc aacctcacca tcgagcagca ggccggcgaa atcgacaagg
      421 tcaagcgctc cgagtccggc atgatcgtcg atcccgtcac catggctccc gagcagctca
      481 tttccgatgc tctcgaggtc atgcgccgct acaaaatctc cggcgttccg gtcacgcgca
      541 acaaaaaact cgtcggtatc ctcaccaatc gcgatctccg ttttgaaacg cgtaccgatg
      601 tccccatcgg tgaggtgatg accaaagaaa acctcatcac cgtccccgtc ggcaccaccc
      661 ttgagcaggc cgaagaaatc cttcaccacc accgcgtcga aaagctcctc gtggtcgacg
      721 agcactacga gctcaagggc ctcatcaccg tcaaggacat ccagaaaaaa ctcaaatacc
      781 ccaacgcctc caaggatgag cagggccgtc tccgcgtcgg cgccgccatc ggcgcaaccg
      841 gcgacttcct cgagcgcgcc gctgaactgg tccgcaaccg ctgcgatgtc cttgccatcg
      901 actcggccca cggccactcc tcgcgcgtcc tcgacgccgt ccgcgaagtc aaaaagcgct
      961 tcccccacgt cgatctgctt gccggcaacg ttgccacccg cgaaggcacc gaagccctca
     1021 tcgatgcggg cgctgacgcg gtgaaggtcg gcatcggtcc cggctctatc tgcaccaccc
     1081 gcatggtcac cggcgccggt atgccccaga tcaccgccat cagcgaagcg tacaaagccg
     1141 ctcagccccg cggcatcccc gtcatcgccg acggcggcat caagtactcc ggcgacatca
     1201 ccaaagcggt cgccgctgga gccagcgtca tcatgatcgg ctcgctcttc gctggcgtcg
     1261 acgagagccc gggcgaaacc atcctctatc agggccgatc ctttaaggcc tatcgcggca
     1321 tgggttcgct ctctgctatg gcccagggcg gcggcgagcg ttatttccag ggaaccaaag
     1381 atctcgacct cgactccagc aacgagagcg tcgtcaaccg ggagcgcaac ggcagctcca
     1441 accgcctcgg caaattcgtc cccgaaggca tcgagggccg cgtgccctac cgcggtgcgc
     1501 tcgaagcgat ggtctaccag ctggtcggcg gcctgcgctc cggtatgggt tactgcggcg
     1561 ccgccaccat cgaagagctg cagcaaaaag ccaaattcgt ccgcatctcc tccgccggcc
     1621 tccgcgaatc ccacgtccac gacgtggtca tcacccgcga agcccccaac taccacgtcg
     1681 aataggcgag cctggcctaa aaccgcgtcg ccatggatgc cccacatgcc tgcagtcggg
     1741 catgtaggta tcgcgcgtaa gcgcgaccgt ttttcctgag ccctgagccc tgcgccctgt
     1801 tagaagcgga tcgcgcgaag cgcgcccgcc cgccccatga acatccctgc aaaacatcta
     1861 tcctgacccg tctcgtcgcc tacaataatc tcggtcaccc accatggccg ccgccgtgcc
     1921 cattccgtct gccaccgaag ccgagcgatt cgtccgctgc tttgacccgc acgccaccca
     1981 cgcggaaggt tcccacgaag ctctcatcct ctcgccgcgc tgggctcgcc tgcgccgcac
     2041 ccaccctctg ctcgcgaccg t
//
LOCUS       DASZYN010000703         1933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_297244, whole genome shotgun sequence.
ACCESSION   DASZYN010000703 DASZYN010000000
VERSION     DASZYN010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297244"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VHE33_18180"
     CDS             complement(<1..474)
                     /locus_tag="VHE33_18180"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HVT99437.1"
                     /translation="MEIPPDVPPQGRVDLRVPPTYLFTRYAELLRNALQREVARLRRP
                     ASALASPVLDSFYGQLGYHLGWLDAHFAPVRQHTGKLLRPTLLLLAYEAAGACETTAR
                     RDEQTHLARALPAAVAIELYHNFTLIHDDIEDGDRERRSRPTAWAVFGLPQALNLG"
     gene            complement(474..1790)
                     /gene="ispG"
                     /locus_tag="VHE33_18185"
                     /gene_synonym="gcpE"
     CDS             complement(474..1790)
                     /gene="ispG"
                     /locus_tag="VHE33_18185"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HVT99438.1"
                     /translation="MKRRTTRQIQVGTVPLGGDAPIAVQSMTTTYTRDVQATVEQIHR
                     LEEVGCELVRVACPEEVDVRALGAIRRQIHLPLIADIHYDGKLALLAIEQGVDAVRIN
                     PGNLLGGREMFASIVTAARAAGIALRIGVNSGSIDALEHGQQMRKIAMKLRDDGTLVR
                     SDPAEERRRAREHLAERMVARALEYCGWAEDLGFSAYKVSLKSSNVLTAVAAYRLFAA
                     ACDVPLHLGITEAGTLVSGAVKSAVGFSQLLGDGIGDTIRVSLTAEPEEEIPVAYEIL
                     RALELRQRGVTFVSCPSCGRVEIDVLAIASEVEKRLARVQTPIQVAVMGCVVNGPGES
                     RDADVGLAGGRGKGVIYRKGQVVKTVPESDFLVEVLKEIASLLPPHEACLVYPGDEAP
                     GSVSLGRTRKSGVHRAPAQQSGAEPPDARTSLRGVSLPLLSQSEVS"
BASE COUNT          386 a          645 c          559 g          343 t
ORIGIN      
        1 gccgagattg agcgcctggg gaaggccgaa aaccgcccag gccgtgggac gagaacgccg
       61 ttccctgtcc ccatcctcga tatcatcatg gatgagcgta aaattatggt agagttcaat
      121 cgctaccgca gcaggcaacg cgcgcgccag gtgggtctgc tcatcgcgcc gcgccgtagt
      181 ctcgcaggct cccgcagctt cataggccag caagaggagc gttggccgca ggagtttgcc
      241 cgtatgctgc cgaaccggcg cgaaatgggc atccagccag cccagatgat accccaactg
      301 cccgtagaat gagtcaagca cagggctcgc cagggcagaa gcaggccttc tcaagcgcgc
      361 aacctctcgc tggagcgcgt ttcgcagcaa ttccgcgtaa cgagtgaaca gatacgtcgg
      421 cggtacccgc aggtccaccc tgccctgcgg aggaacgtcg ggcggaattt ccatcaggac
      481 acctcgcttt gggaaaggag aggcagcgag actccccgca aggacgttct ggcgtcggga
      541 ggctccgctc cactttgctg agcgggagcg cgatgaacgc cgctcttgcg cgtgcgcccc
      601 agactcaccg aaccgggagc ctcatcgcct ggatagacga ggcacgcttc atgagggggc
      661 agcagactgg cgatctcctt gaggacttcc acgagaaaat cgctctcggg gacggttttg
      721 accacctgtc ccttgcgata gatcacgccc ttgcctcgtc ctccggccag gcccacatcg
      781 gcgtcccgcg actctccggg tccattgacc acacagccca tcaccgcaac ctggatgggg
      841 gtctgcaccc tggcaaggcg tttctcgacc tcgctggcaa tcgccagcac atcgatctcc
      901 acgcggccac aggagggaca ggagacaaag gtcacgcctc gctgacgcaa ctccaaggcg
      961 cgcaggatct cataggccac cggaatctcc tcttccggct cggcagtcaa cgaaacccgg
     1021 atagtatcgc cgatgccatc gccaagcagt tgtgaaaacc ccaccgctga cttgaccgcg
     1081 ccactgacca gggttccggc ctcggtgatg cccaggtgga gaggcacatc acaggcggca
     1141 gcaaacaggc gataagccgc gacagccgtc agcacattgg acgatttgag cgagacctta
     1201 taggccgaaa agcccagatc ttcggcccat ccacaatact ccagcgcgcg ggctaccatg
     1261 cgctcggcca ggtgctcacg tgcgcgccgt cgctcctcgg cgggatcgga gcgtaccagc
     1321 gtgccatcat cgcgcagctt catcgcaatc ttacgcatct gttgtccatg ctcaagggca
     1381 tcgatagaac cggagttgac gccgatgcgc agggcaattc cggcagcgcg ggcagccgta
     1441 acaatgctgg cgaacatctc gcgtccaccg agcaggttgc cgggattgat gcgcacagca
     1501 tccactccct gctcgatagc cagcagcgcc agcttcccat cgtagtgaat atccgcaatc
     1561 aacggcagat gaatctggcg cctgatcgcc cctaacgccc ggacatccac ttcttccggg
     1621 caggccacac ggaccagctc gcagcccact tcttccagac gatggatttg ctcaaccgtt
     1681 gcctgaacat cgcgcgtata cgtcgtggtc atcgactgga ccgcgatggg cgcgtctccc
     1741 ccaagcggga ccgtccccac ctgaatctgc ctggttgttc gtcgcttcat tcctcatccc
     1801 ctttgcgcag aggcaccagc ggctctgtct gtgttgtgtg cgggtcgtag gcttccaggt
     1861 accgaatgac ctcgcgtgtc aggtggctgg gcgtcgaggc acccgccgtc accgcgaccc
     1921 gtttttttcc agc
//
LOCUS       DASZYN010000704         7846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_808076, whole genome shotgun sequence.
ACCESSION   DASZYN010000704 DASZYN010000000
VERSION     DASZYN010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7846
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808076"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VHE33_18190"
     CDS             <1..484
                     /locus_tag="VHE33_18190"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HVT99439.1"
                     /translation="ALGDQLVAEMTDAVAQMRTYTADLPQPRVYCEEWGKPLIHSQLW
                     VRELVEAAGGIFLGEPGAQTTPEAIAAANPDVILAAWCGAGDRVPLEKLAARPGWSNL
                     RAVRDGRLFCIPDELLNTPAPTLIAGLSAIASALHPAVFGTSGIIRALKQHPSIPAGV
                     "
     gene            498..926
                     /locus_tag="VHE33_18195"
     CDS             498..926
                     /locus_tag="VHE33_18195"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HVT99440.1"
                     /translation="MADTAQLRDPKTLRFVAAALILRGDEVLVCQRKAGSPMGLKWEF
                     PGGKIEAGESAEQALRRELNEELGIDAQVGTRIAHLRHTYRTGGAVDLQFFAVHSFTG
                     DITNHIFEDVRWTPLRTLPDYDFLAADRDLVRDLAAGKLL"
     gene            1656..1904
                     /locus_tag="VHE33_18200"
     CDS             1656..1904
                     /locus_tag="VHE33_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99441.1"
                     /translation="MPLCPLLGMVNGIATPYACTMNFKQGQKVRIKGKHVVGTVEKKL
                     TSLDDVWVIVLEHHSVGEEKLVRGADLEAIDVDKQKTA"
     gene            complement(2021..2731)
                     /locus_tag="VHE33_18205"
     CDS             complement(2021..2731)
                     /locus_tag="VHE33_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99442.1"
                     /translation="MDTQTRHALKQDKFVNVTTSGLEWVGEHRGNVIAWSVGVVLLVA
                     IAVVSLVLFQKREAAADQMLGQAMDIYETPLTQPDQPAEAGLKTYATAADRAKAAYPL
                     FRQTADRYGWLTAGQMARYFAGLTELDLGQKSQAEADLEKATHAGNGNLAALAKIALA
                     NLYEQTERNSQAVAQFQDVIAHPTTTVPKSEAQLQLAQLYETTQPQEARRLYAEIKDQ
                     DKQSAAAQIATQKLQGLK"
     gene            complement(2928..3314)
                     /locus_tag="VHE33_18210"
     CDS             complement(2928..3314)
                     /locus_tag="VHE33_18210"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HVT99443.1"
                     /translation="MTRPSQNSKQRRFEERLRHQQQQLERTMLTAVKEGRKAVAEDTL
                     DPADQAVLSYQKELIFSQGTEGHSQLSLVRLALERLREGTFGDCLNCGTAIGEKRLEA
                     LPWTPYCIDCQEKVENGEIDDVVRAA"
     gene            3527..3922
                     /locus_tag="VHE33_18215"
     CDS             3527..3922
                     /locus_tag="VHE33_18215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99444.1"
                     /translation="MYEDFHAIDRWTGEDLHCRWKANIVAIATRHADAVDIRFDVNRR
                     PVWIAMPCTAWVEQKKRTGHVITDSLAAQAAGLYLKKAIESGYDNGREMYTMTIEEVL
                     QHVDAVLHEAGHTRNLPWLVPMDDAQPQV"
     gene            4051..5649
                     /gene="pckA"
                     /locus_tag="VHE33_18220"
     CDS             4051..5649
                     /gene="pckA"
                     /locus_tag="VHE33_18220"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HVT99445.1"
                     /translation="MGATATLQDLKRLTEGLHAHRNLSTPQLVETAIARGEGRLASNG
                     ALVAMTGARTGRSPRDKFTVEDDETRERVHWGKVNQAFPPEKFEALLQRVIRHMAERD
                     LFIMDLAAGADPNYRLPIQVICEYAWHALFVKQLFIRPDRQELASHKPEFIVIAAPEF
                     EAIPERDGVRSSTFILADFTRRIILIGGTKYAGEMKKSIFGVMNYILPPRNVFPMHCS
                     ANTAPDGTTALFFGLSGTGKTTLSADPDRRLIGDDEHGWSPDGVFNFEGGCYAKCVDL
                     TIEKEPQIYNAIRFGSVLENVVLDPRTGEPDYSDIRYTENTRAAYPVDYIANAVIPGV
                     GGHPRNVLFLAADAFGVLPPIAKLTPDQAMYHFLSGYTAKLAGTEAGLGSEPVPEFST
                     CFGSPFLPLAPGVYAEMLGRRLREHHAQCWLVNTGWTGGAFGVGKRMSLPHTRAIVHA
                     ALDGRLAKAEYTIESAFGLSIPTECPGVPSELLNPRNAWADKHAYDAQARLLASKFVE
                     NFKRFHVPDHVRQAGPQVPTQTQR"
     gene            complement(5646..6938)
                     /locus_tag="VHE33_18225"
     CDS             complement(5646..6938)
                     /locus_tag="VHE33_18225"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HVT99446.1"
                     /translation="MSNADLTAVLFLLLLLVALAQLLGWVFTKLRQPRVVGEILAGVL
                     LGNAVAGRWPAAAHFIAHARQQGDVLDFVYWLGLLLLMFLAGAETQQLFTRDDRRSVG
                     WLVILGTGLPFAAGLLLAPWLIRPSLAGPAGTRISLTIVLAVGVAVTSVPVVSKIFAD
                     LRILHTRFARLVLGVAVIEDIVLWLALAIATAVAGKAVLHPRAMGMHLAATVAFFVLG
                     LTLLPRAIKRLNKARFNVIARSSPTGYALLVLLGYCAIAGALDVSLVFAAFLAGFAVV
                     HRKRRLFAEALDAIGKVSFAFFVPVYFAIVGLKLDLIRGVSLGMIAAFVLGTCAVKIL
                     SVGLAGRFAGFRGLDLLNLAITTNARGGPGIVLASVAFDAGIISSKFYTTLVIAAVFT
                     SQVAGAWLEYVLRRGWPLLTPAELAEPATVAPEKPAAA"
     gene            7252..7659
                     /locus_tag="VHE33_18230"
     CDS             7252..7659
                     /locus_tag="VHE33_18230"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HVT99447.1"
                     /translation="MQNHPYIWNYLPLLMQIVVVGALSGGMVGMSWFIGRHRNLKSKL
                     EPYECGIPVEGDARGRFSVRFYMVAMLFILFDVEAVFMIPWAVIYRQLPHIAGSRLFG
                     FWEMLVYLGFVVIGIFYVWKKGILDWASDKADL"
BASE COUNT         1544 a         2650 c         2170 g         1482 t
ORIGIN      
        1 cgccctgggc gatcagcttg ttgccgagat gaccgacgcc gtcgcccaga tgcgcaccta
       61 caccgccgac ctgccccagc cccgcgtcta ctgcgaggag tggggcaagc ccctgatcca
      121 ctcccagctc tgggttcgcg agctggtcga agccgccggc ggcatcttcc tcggcgagcc
      181 cggcgctcaa accacccccg aagccatcgc cgccgccaat cccgatgtca tcctggccgc
      241 ctggtgcggt gccggcgacc gcgttcccct ggaaaagctc gccgcgcgcc ccggctggtc
      301 gaacctccgt gctgtccggg acggccgcct cttctgcatt cctgacgaac tcctgaacac
      361 gcccgcaccc accctcatcg cgggcctgag cgccatcgcc tcggccctcc accctgcggt
      421 tttcggtact tccgggatca ttcgcgccct gaaacagcat ccatccatcc ccgcaggcgt
      481 ctaaactgag ggacaaaatg gcagatacgg cccagctccg tgacccgaag accctccgct
      541 tcgtggccgc cgctctcatc ctgcgcggcg acgaggtcct ggtctgtcag cgcaaagccg
      601 gctccccgat gggcctcaaa tgggagttcc ccggcggcaa aatcgaggcg ggcgaatccg
      661 ccgagcaggc cctccgccgc gaactcaatg aggaactggg catcgacgcc caggtcggca
      721 ctcggatcgc tcacctccgc cacacctacc gcactggagg cgccgtcgac cttcagttct
      781 tcgccgtcca cagcttcacc ggcgacatca cgaaccacat ctttgaggat gtccgctgga
      841 cccccctccg caccctgccg gactacgatt tcctcgccgc cgaccgcgac ctcgtccggg
      901 atttagccgc cggcaagctg ctctagcggg aagtcatcct gaagggaagc cggtcgcgaa
      961 gcggccaatg gaaacctcaa gccgagtctc accaaaaagc tgcattcaac gcgaaaaatg
     1021 ccgacctcac gggctcatcg caaaactgtc aagaggcgaa ccgcttcacc aaagtaatgc
     1081 ccgttgattc caggccgctt ccacgccaaa tcaccccaaa aagtttggca cgatttccct
     1141 ctcaacctgg tattctgaaa ataggggacg gaaagcactc tttcgtctcc cacgacgttc
     1201 cacccaggct cgccgaggcg agcttttttc ttgtggaaaa actcatcacg gggaaagcgc
     1261 tctttgatat tcggagaacc acggcacacc ttgccgcaga ttcaacttcg cggccgacgg
     1321 tgtcacattc accgccgtgt ttgtaataca aacggccgcg agtgtaacaa acacagccgt
     1381 ctttgttatc aaaacggccg tttcatcgat gccaagggcc cttcttaagt tctttagatt
     1441 gaataagaaa aagttgtcga gtttgtcaac agaagcaaat ctctcagaat cagcaacatg
     1501 caacttgtct cgcgtgaagt ccacaacacg ctcgtccgcc gaccatcatc gcgctggcga
     1561 cgcccagtct tccacagccg gctctcatta ccttgcaacg taccgtagtt ctcaatcaca
     1621 tcactatcgt gatgagccaa gattactgtt ctggaatgcc cctttgcccc cttctcggga
     1681 tggtgaacgg aatcgctaca ccctacgctt gcaccatgaa cttcaaacaa ggacaaaaag
     1741 tccgtatcaa gggcaagcac gttgtcggga ccgtcgagaa gaagctcact tcgctcgacg
     1801 acgtttgggt catcgtcctc gagcaccact cggtgggcga ggaaaagctc gtccgtggcg
     1861 cagacctcga agccatcgac gtcgacaaac agaagaccgc ctgagaagcc cgagccatcc
     1921 gctcctcccc ggaagagcag cggcctcggc gcgaactccg tgccagtaaa aatgggcgcc
     1981 gaagcgccca tcgtctttct gctccctgcg ctctgcgccc ttacttcagt ccctgcagct
     2041 tctgcgtcgc aatctgcgcc gctgcggact gcttatcctg gtccttgatc tcagcgtaga
     2101 gccgccgcgc ctcctgcggc tgcgtcgtct catagagctg agccagctgc aactgcgcct
     2161 cactcttcgg cacggtcgtc gtcggatgcg cgatcacatc ctggaactgc gcaaccgcct
     2221 gtgaattgcg ctccgtctgc tcgtacagat tcgccagcgc gatctttgcc agcgccgcca
     2281 gattgccgtt gccggcatgc gtcgcctttt ccagatccgc ctccgcctgc gacttctgcc
     2341 ccagatccag ttcggtcagt ccggcaaagt aacgagccat ctgcccggcc gttagccagc
     2401 cgtaacgatc cgcggtctgc cggaaaaggg gataggcggc tttggcccga tccgcggccg
     2461 tagcgtacgt cttcagcccc gcctctgccg gctgatccgg ctgcgtcagc ggcgtctcgt
     2521 agatgtccat cgcctgcccc agcatctggt ccgctgccgc ctcccgcttc tggaaaagga
     2581 caaggctgac aaccgcaatc gccaccagca gcactacgcc gaccgaccac gcgatcacgt
     2641 tgccgcggtg ctccccgacc cattccaggc cggaggtggt tacattgacg aacttgtcct
     2701 gtttcagagc gtgacgggtc tgtgtgtcca ccggttttgc cgcgtttcct ttgagcggga
     2761 gtcgcgaaca cgcacctcag gcgcatccgc aagatactcc tcagtctagc acgcactcga
     2821 cgggctgccc gggtagggag cgctcaggcc agggtcgagt cacaggacct gcgtacaagg
     2881 ttctgagcac cggctaccgg gccctgcgac ctgtgatctg cgacctgtca ggcggctcgc
     2941 acgacgtcgt caatctcgcc gttctccacc ttttcctggc agtcgatgca gtatggcgtc
     3001 cacggcagcg cctccaggcg cttctcaccg atcgctgtgc cgcaattcag acaatcgccg
     3061 aaggtcccct cacgaagccg ttccagcgcc agccgaacca gcgagagctg gctatggccc
     3121 tccgtaccct gagagaagat cagttctttt tgatacgaca gaacggcctg atcggccgga
     3181 tccagcgtat cctcagccac agccttgcgg ccctctttca ccgctgtgag catcgtgcgc
     3241 tccagttgct gctgttgatg gcgaagccgc tcttcgaatc gccgctgttt ggagttttgc
     3301 gatggacggg tcacggagga aacctcggaa taagggctgt cccgcgttct ggtcgggctc
     3361 aggacataca ttggacgcac aaagtcccct aagatttgtt tttctttttg cactttcccg
     3421 gggtttattg tctggttaac ccctcccctc cctgggtgac gccggtttct cccccgcccg
     3481 ccaaccgggt gcgccgcgcc gcaaaatgct ctaggctgaa agagccatgt acgaagactt
     3541 tcatgccatc gaccgctgga cgggcgaaga tctgcactgc cgctggaagg ccaacatcgt
     3601 cgccatcgcc acgcgccacg ctgacgcggt cgacattcgc ttcgatgtca atcgccgtcc
     3661 ggtttggatc gccatgccct gcaccgcctg ggtggagcaa aagaaacgca ccggccacgt
     3721 catcaccgac tccctcgccg ctcaggctgc cggcctctat ctgaagaaag ccatcgaatc
     3781 cggatacgac aacggccgcg agatgtacac catgacgatc gaagaggtcc tgcagcacgt
     3841 cgacgctgtg ctccacgagg ctggccacac gcgcaatctg ccctggctcg taccgatgga
     3901 cgacgcccag ccgcaggtgt aagcctgtcg catccggcgg ctgttcactc accggctgcc
     3961 gccacgatcg cggtgtcgtt tttctctttg cagggccacc ccccaatggg acaataatgg
     4021 gttctctctg acccacggag gcttccaacg atgggagcaa cagccactct gcaggacctg
     4081 aagcgcctga ccgaaggtct gcacgctcac cgcaatctat cgacgcctca actggtggaa
     4141 accgccatcg cgcgcggtga gggccggctg gcatcgaacg gtgcgctcgt agccatgacc
     4201 ggcgcccgca ccggccgctc tccccgcgac aagttcaccg ttgaggacga cgagacgcgg
     4261 gagcgcgtgc actggggcaa agtgaaccag gccttcccgc ccgaaaagtt cgaagcgctg
     4321 ctgcagcggg tcatccgcca tatggcggag cgtgacctct tcatcatgga tctggccgcc
     4381 ggcgctgatc caaactaccg cctgcccatt caggtcatct gcgaatatgc ctggcacgct
     4441 ctgtttgtga agcagctttt catccgcccc gaccggcagg aactggccag ccacaaaccc
     4501 gagttcatcg tcatcgcggc tcccgaattc gaagcgatcc cggagcgcga cggtgtccga
     4561 tcctccacgt tcatcctcgc cgacttcacc cgcaggatca tcctcattgg cggtaccaag
     4621 tatgccggcg agatgaagaa gtccatcttc ggcgtgatga actacatcct cccgccccgc
     4681 aacgtctttc ctatgcactg ctcggcgaac accgcccccg acggcacgac cgctctcttc
     4741 tttggactgt cgggtaccgg caagaccaca ctctccgccg accctgaccg ccgcctcatc
     4801 ggcgacgatg agcacggctg gagcccggac ggcgtcttca acttcgaggg cgggtgctac
     4861 gccaaatgcg ttgacctgac gatcgagaaa gagccgcaga tctacaacgc cattcggttc
     4921 ggttcggtcc tggaaaacgt cgtgctcgac ccccgcaccg gcgagccgga ctactccgat
     4981 atccgctata ccgaaaacac tcgcgctgcc tacccggtcg actacattgc gaacgccgtc
     5041 atccccggtg ttggaggtca tccccgcaac gtcctctttc ttgccgcaga tgcgttcggt
     5101 gtcctgccgc ccattgccaa actgactccc gaccaggcga tgtatcactt tctctcgggg
     5161 tataccgcga aactcgccgg aaccgaagcc ggtctgggca gtgaacctgt ccccgaattc
     5221 tccacctgct tcggctcgcc gttcctgccg ctcgcccctg gtgtttacgc ggaaatgctc
     5281 ggccgccggc tgcgcgagca ccacgcgcag tgctggctgg tcaacacagg ctggaccggc
     5341 ggcgcgttcg gggtagggaa gcgcatgagc cttccgcaca cacgcgccat cgttcacgcc
     5401 gcccttgatg gtcgtctcgc gaaagcggaa tacaccatcg agtctgcgtt tggactcagc
     5461 attccaaccg aatgccccgg cgttccctca gaattgctca acccgcgcaa tgcctgggcc
     5521 gacaagcacg cttatgacgc gcaggctcgc ctgctggcct cgaagtttgt cgaaaacttt
     5581 aagcgattcc acgtgccgga tcacgtgcgc caggccggtc cgcaggtgcc aacgcaaacg
     5641 cagcgttagg ccgcggcggg tttctcgggt gcaactgtgg ctggttcggc gagttctgct
     5701 ggcgtcagga gaggccatcc cctgcgcagc acgtattcca gccacgcacc cgcgacctga
     5761 gaagtgaaca ccgcggcgat gaccagagtc gtatagaact tcgagctaat gatgcccgca
     5821 tcgaatgcca cgctggccag cacaatgccc ggtccgccgc gagcgttggt cgtaatcgcc
     5881 aggttgagca ggtccaggcc gcggaatccg gcaaaccttc ccgccaggcc tacggagagg
     5941 attttcacgg cacaggtccc caggacgaag gccgcgatca tgccgagcga cacgccgcga
     6001 atcaggtcca gcttgagccc gacaatagcg aagtagaccg gaacgaagaa ggcaaaggag
     6061 actttgccga tcgcatcgag ggcctctgca aaaaggcggc gcttgcggtg gaccacggca
     6121 aatcccgcca gaaacgccgc aaagacgaga cttacgtcga gagctccggc aatcgcacaa
     6181 tagcccagca gcaccagcaa cgcgtaaccg gtgggcgagg accgtgcgat cacgttgaaa
     6241 cgcgccttgt tcagtcgttt gatggcgcgg ggcagcaggg tcagccccag cacgaagaat
     6301 gccacggtcg cggcaaggtg catgcccatg gctcgtggat gcaggacagc cttgcccgct
     6361 acggccgtgg caattgcgag ggccagccac agcacgatgt cctcgatcac cgcgactccc
     6421 agcaccagcc gtgcaaaccg cgtgtgcagg attctgaggt ccgcaaaaat ctttgagacc
     6481 accggcaccg aggtgaccgc cacccctact gccagaacga tggtcaggga gatccgggtt
     6541 cctgctggcc cggccaggga aggacgaatc agccacggag cgagcaggag gcccgcggca
     6601 aacggcaacc ctgttccaag gatgaccagc cagccgacgc tgcgccgatc gtcgcgagtg
     6661 aacaactgct gcgtttcggc tcctgccaga aacatgagca acagaagccc cagccagtag
     6721 acaaagtcga gcacatcgcc ctgttgtctg gcgtgcgcga tgaagtgagc cgccgcaggc
     6781 caacgtcccg ccacggcgtt tcccagcaga acccccgcca gaatttcacc gaccaccctg
     6841 ggctgtcgca gcttggtaaa gacccaccca aggagctggg cgagcgcaac cagcagcaga
     6901 agcagaaaaa gcacggcagt caggtcggcg ttcgacatgg cgatatcctg gctcctcgga
     6961 tgccgctcga ttggaatcgt ttgtatgcac aagcaccccg tcgctcccgc tgccgggttc
     7021 ggcttcgcgc tgccacaggg caggttttca gctcatacat ttggtcgacg agaggtatct
     7081 gagggcgcga ccccgttctc ctgggagact tcagcgacct gctgcgagca acccgatacc
     7141 ggttgttcct cggtaacttg actcttgaca accgtagact tagtctcgaa atggaggcag
     7201 cggcagttcc ccttcacctc cgctcggttg tcgttgtcca ggagaacggc cttgcaaaac
     7261 cacccctaca tctggaatta ccttcccctg ctgatgcaga tcgtggtggt tggcgctctc
     7321 tccggcggga tggtcggcat gtcctggttc atcggccgcc accgcaatct gaaaagcaag
     7381 ctcgaaccct atgagtgcgg cattccggtg gagggcgacg cgcgcggacg tttctccgtg
     7441 cgcttctata tggtggcgat gcttttcatc ctgttcgacg tggaagccgt cttcatgatc
     7501 ccctgggccg tgatttaccg gcagctgcct cacattgccg gctctcgtct ctttggcttc
     7561 tgggagatgc tcgtctatct cggattcgtc gtcatcggta tcttctacgt gtggaagaaa
     7621 ggcatcctcg actgggcctc cgacaaggct gacctctaac ccaaacctcc agccctgcag
     7681 aaggattcat gggagaagcg ctgctcaata aagaagctgt gttcgagagc ctcgcgcaga
     7741 atgtggccat ccaggccatt cgcgctgcgc atcccgacgt cattaccaac gcgaaattcg
     7801 atcgcgggga actgacgctc gtccttgccg ccgaccagat tcgtac
//
LOCUS       DASZYN010000705         2378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_235905, whole genome shotgun sequence.
ACCESSION   DASZYN010000705 DASZYN010000000
VERSION     DASZYN010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235905"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..769)
                     /locus_tag="VHE33_18235"
     CDS             complement(29..769)
                     /locus_tag="VHE33_18235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HVT99448.1"
                     /translation="MTIHVVLADDEVLARQKLRLLLREQTDCEVVGECATAAETVALV
                     RATHPDVVFLDIRMPDMDGFDVVDALSAEPEFTMPRIVFTTAYDQYAVRAFDVHAVDY
                     LLKPFAPQRLQTTLQRIRERGNGGAAVGGEKKGGRYTSRIVFKSKGRILFLPVSEIRW
                     IAAEENYVRIFTENETHLLRETMARLEERFDPQLFLRVHRSAIVNLQHVREVRTESDG
                     ETAVILLNGQKVPMSRSYRSRMQRLLHR"
     gene            complement(766..1905)
                     /locus_tag="VHE33_18240"
     CDS             complement(766..1905)
                     /locus_tag="VHE33_18240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HVT99449.1"
                     /translation="MKGPQENTQSERPLFMHPAVFIGSSVLVGLLFALQEYLNARRWG
                     YKIALLFLTEMWGMQFLLWGVICWMLWRFLRSFVVNAGIGALLTRMLPLSIVLSVVEE
                     MIWVVFFPRVPIDRPPMDYWHRFTFQLNAEFVDSMAIFWCAFIVIRGVAYYQKFREKE
                     TTAARLQVQLVQARLAALRMQLNPHFLFNAMNSISSLMRTDVNAADTMLEQLSSLLRI
                     TLERGDVQLVPLHKEVEFVEIYMAMQQQRYGGKVSQTLSVEPELHDALVPALILQPIV
                     ENAYVHGVSRCRENADLAIDIRRDGKRLKMSVVNSGNGLTPERVEPDRRGVGLANVRD
                     RLRLHYGERGSLSVREVGVGRVNVTIALPLQMDGNGTEEAARFDA"
     gene            complement(1957..>2378)
                     /locus_tag="VHE33_18245"
     CDS             complement(1957..>2378)
                     /locus_tag="VHE33_18245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99450.1"
                     /translation="LEDHPELAKSDREFLHLFEQAAGLGQWKSSLALGMLFNEGKLVP
                     RDPGRAYYYLELAALQGGDSAPLQTLSQMQQKLSNETGNEEASKQESAARGWYREHPD
                     RIEQLMRDHGKDSSNKYAIAVPAEGIHAGQIVNQPAS"
BASE COUNT          505 a          728 c          647 g          498 t
ORIGIN      
        1 tccggtctac agaaaagtgg atcgacgatc agcgatgcag cagacgctgc atccgcgagc
       61 ggtagcttcg gctcatcggc accttctggc cgttgaggag aatgactgcg gtttccccgt
      121 ccgactccgt tcgcacttcc ctgacatgct ggagattgac gatcgcggat cggtgaacgc
      181 gcagaaagag ctgaggatcg aaccgctcct ccagccgggc cattgtctcg cgcaaaagat
      241 gagtttcatt ctcggtaaag atgcggacgt aattctcctc cgcggcgatc caccgaatct
      301 ccgagacagg cagaaaaagg atgcggcctt tggatttgaa aacgatccgg ctcgtgtatc
      361 gtccgccctt tttttccccg cccacagcgg ctccgccgtt tccacgctca cgaatacgct
      421 gcagggtggt ctgcagccgc tgtggcgcga acggcttcaa caaatagtcc acggcgtgga
      481 catcgaaggc acgcacagca tactggtcgt aagcggtcgt gaagacgatg cgcggcatag
      541 tgaactccgg ctctgccgac agcgcatcga ccacatcgaa gccatccata tccggcatgc
      601 ggatgtcgag aaacaccaca tcgggatggg ttgcgcgcac cagcgcaacc gtctcggccg
      661 ccgtggcgca ttctccaacc acctcgcagt ccgtttgttc ccgcaaaagc aggcgcagct
      721 tttgccgcgc cagcacttca tcgtcggcca gaacaacgtg gatggtcatg cgtcaaaccg
      781 tgcggcctct tctgtgccgt tgccatccat ctgcagggga agggcgatcg ttacgttgac
      841 gcgccccaca cccacttcac gaaccgagag cgatccgcgc tcgccgtaat gcagccggag
      901 acggtctctg acattcgcga gccccacacc gcggcgatcg ggttccaccc gctccggagt
      961 caggccgttc ccgctgttga cgacgctcat cttgagccgt tttccatccc tgcggatatc
     1021 gatggcgagg tctgcatttt cccggcatct ggaaacgcca tggacatagg cgttttccac
     1081 gatgggttgc aggatcagcg ccggcaccag agcgtcatgc agttccggct ccaccgaaag
     1141 cgtctggctc accttgcctc cataccgttg ttgctgcatg gccatataaa tctccacaaa
     1201 ctcgacttcc ttgtgcagag ggaccaactg cacgtctcct cgttccagcg tgatacgcaa
     1261 cagactgctc agctgttcca gcatcgtgtc tgcggcgttg acatccgtcc gcataaggct
     1321 cgaaatgctg ttcatcgcgt tgaagagaaa atgcggattc agttgcatgc gaagggccgc
     1381 cagtctcgcc tgaaccagct gcacctgcag gcgggctgct gtcgtttcct tctcccggaa
     1441 tttctggtaa tacgcgactc cgcggatgac gatgaatgca caccagaaga tggccatgct
     1501 gtcgacgaac tcggcattca gttgaaacgt gaaccggtgc cagtagtcca tcggtggtcg
     1561 gtcgatgggc actctgggaa agaagaccac ccagatcatc tcttccacaa cactgagcac
     1621 aatgctcagc ggaagcatgc gcgtcaacaa cgcgccaata cctgcgttca ccacaaagga
     1681 ccgaagaaaa cgccacagca tccagcagat gacaccccac agcaggaact gcatgcccca
     1741 catctcggtt agaaagagga gggcgatctt gtatccccag cggcgggcat tgagatactc
     1801 ctgcaaggca aacagcagcc ccaccagcac cgacgagccg atgaacaccg ctggatgcat
     1861 gaacagcgga cgttccgatt gagtgttttc ctggggtccc ttcacctgga ttgctctttg
     1921 tcctcctgca atacaactgc aacgaaattt gtggcttcag gatgccggct ggttgacgat
     1981 ctgcccggca tggattccct cagcggggac agcaatggca tatttgttgc tcgaatcctt
     2041 tccgtgatcc cgcatcagct gttcgattct gtcgggatgt tcgcgatacc atcctcgcgc
     2101 tgcagattcc tgctttgaag cttcttcgtt tcctgtttcg ttcgagagct tttgctgcat
     2161 ctgggaaagg gtctgcaggg gggcgctgtc gcctccctgc agagcggcca gctccaggta
     2221 atagtatgcc cttcccggat cgcgcgggac gagctttcct tcattgaaaa gcatgccgag
     2281 cgcgagagag gatttccact gaccgagacc ggcagcctgc tcgaacagat gcagaaattc
     2341 gcgatccgat ttcgccagct caggatgatc ctcaagaa
//
LOCUS       DASZYN010000706         2762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_808101, whole genome shotgun sequence.
ACCESSION   DASZYN010000706 DASZYN010000000
VERSION     DASZYN010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808101"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..1282
                     /locus_tag="VHE33_18250"
     CDS             350..1282
                     /locus_tag="VHE33_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HVT99451.1"
                     /translation="MMHIICGVDVSKSWLDSFVAGTHRRFANSRQGCQELLEFCREQA
                     AELVVMEASGGLEQPAFLLLWQLGQPTALANARSVRRFAEAMGALEKTDRIDAQMIAR
                     FAQAKQMLPTPPPSADQQRLNALVTRLRQVTEDLGNQKRRLHSARDALARASLNEAIA
                     FFKAQVRDLADQIAALIQTDPLWSALDRTIRSVKGAADRTVAYLLAELPELGTLSNRA
                     ISKLVGLAPLPNDSGHRSGKRPIRGGRAGIRSLLVLVADTVRKFDTSLALFRQRLIDK
                     GRAKMQVRVALAHKLLVRLNAKARETRAALALNA"
     gene            1379..1765
                     /locus_tag="VHE33_18255"
     CDS             1379..1765
                     /locus_tag="VHE33_18255"
                     /inference="COORDINATES: protein motif:HMM:NF039363.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6163 family protein"
                     /protein_id="HVT99452.1"
                     /translation="MDFSSPLAGRYVRIVAIIALLLGLNDASRLLGVSTGSQSPIQLL
                     GVTGFVYLSIFALSRLFAAVGLWIRASWGAVLLVGATGVELGMYMLGSHDIQMSALGF
                     AVRLVLLVSIVLIFILSLRMRERARD"
     gene            1954..2463
                     /locus_tag="VHE33_18260"
     CDS             1954..2463
                     /locus_tag="VHE33_18260"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HVT99453.1"
                     /translation="MATKSNAVEAEAPGSAGSLKPQYLEAVSRVERLHRRLLDLIKDE
                     FDRMGWDDINPVQALLMFNIGDAEMTAGELRSRGYYLGSNVSYNLKKLVETGYIFQER
                     SRADRRSVRIRLTPKGEEVAEVIDELYDRHLKSIDKVGGLDSGTFEGLNKALGRLERF
                     WVDQILYKL"
BASE COUNT          477 a          903 c          857 g          525 t
ORIGIN      
        1 ctccaccagc cttcgctggt ccccctcccc cataaacggg agaggttcgc cgactgcgag
       61 agaggtgcct gggccacccc ctccgtcgcc ttttccccgg gctctgtccg aacgagcccg
      121 agggggctcg ctcggccaac cgcacaggga cgccaccttg tatctgcatc ttgcgctgaa
      181 gtgagctact gctgcgcagc gaggggtcgg tggtcgccgg cccctcgcgc ggcggtgtcg
      241 cccgttccac ctcaggatca cacccgcggg gaagcttggg atcatcgctt ttgtcacgca
      301 caaccgaaca gtcgcttgat catcaatcgt acggacaagg caggttaccg tgatgcacat
      361 catctgtgga gtggacgttt ccaaaagctg gctggatagc tttgtggctg gcacgcatcg
      421 gcgcttcgcc aacagccggc aaggttgcca ggagctcttg gagttctgcc gcgagcaagc
      481 cgccgagctg gtggtgatgg aggccagtgg cgggctcgag cagccggcct tcctactact
      541 gtggcagttg gggcagccca cggcgctggc caatgcccgc tcggtgcggc gcttcgccga
      601 agccatgggg gcattggaaa agaccgatcg catcgatgcc caaatgattg cccggttcgc
      661 ccaggccaag cagatgctcc ccaccccgcc accctcggcc gatcagcagc ggctgaatgc
      721 gctcgtcacg cggctgcgcc aggtcaccga ggatctgggc aaccagaagc ggcgcctcca
      781 tagcgcccgc gatgcgctgg ccagggccag cctgaatgag gctatcgcct tcttcaaggc
      841 ccaggtccgg gacctggccg accagatcgc cgccctgatc cagaccgacc cgctgtggag
      901 cgccctcgat cgcaccatcc gctcggtcaa aggcgccgcc gaccggactg tcgcctacct
      961 gctggccgag ttgcctgagt tggggaccct ctccaaccgc gccatcagca agcttgtggg
     1021 gcttgcgccc ctgcccaacg acagcggcca caggtccggc aagcggccca tccgcggcgg
     1081 ccgcgccggc atccgatcgc tgctggtgct ggtcgccgac accgtgcgca agttcgatac
     1141 cagcctcgct ctgttccgcc agcgccttat cgacaagggc cgcgccaaaa tgcaggtccg
     1201 cgtcgccctc gcccacaaac tgctcgtccg cctcaacgcc aaagcacgag aaaccagagc
     1261 cgctctcgcc ctcaatgctt gacactccag atagtcgctc cccccgcaag cgggagaagg
     1321 atccgactgc ttgccccgcg gtgttgttcg aagcgacggt aagggctaat cctcggccat
     1381 ggacttcagt tcccccctcg ccggccggta cgtccgcatc gtcgcgatca tcgcgctgct
     1441 gctgggcctc aacgacgcgt cgcgactgct gggggtgtcc accgggtcgc agagcccgat
     1501 ccagctcctg ggcgtcaccg gcttcgttta tctctccatc tttgccctga gccggctgtt
     1561 cgccgcggtg ggcctgtgga tccgggcgag ctggggggcg gtgctgctgg tcggcgccac
     1621 cggcgtggag ctcggcatgt acatgctcgg cagccacgat atccagatga gcgcgctcgg
     1681 ttttgccgtc cggctggtgc tgctggtgtc gatcgttctg atcttcatcc tgagcctcag
     1741 gatgcgcgaa cgggcgcgcg attgagcccg gatacgaagc atttagctta ctgatttctc
     1801 gcctcgggtt accttctgta aagccaaaat cccgaacttt ttcagtaaga tagtcttaag
     1861 tgtgcggctt aagtcgatct tattcgggtg cggctagatt gcgtcctatg agaggcgatc
     1921 aatcgcccgg accataggaa cagtgaggca agaatggcaa ccaaatccaa tgccgttgag
     1981 gcagaggcgc cgggcagtgc gggaagtctg aagccgcagt atctcgaggc agtcagccgc
     2041 gtggagcggc ttcaccgccg cctgctcgat ttgatcaagg acgaattcga ccgcatgggt
     2101 tgggatgaca tcaacccggt gcaggcgctg ctgatgttca acatcggcga cgccgagatg
     2161 accgccggcg agctgcgctc ccgcggttac tatctggggt cgaacgtctc gtacaatctc
     2221 aagaagctgg tcgagaccgg ctacatcttc caggagcgca gccgcgccga ccgccgttcg
     2281 gtccgcatcc gcctgacgcc caaaggggaa gaggtcgccg aggtgatcga cgagctctat
     2341 gaccgccacc tcaagtcgat cgacaaggtc ggcgggctgg acagcggcac cttcgaaggc
     2401 ctcaacaagg cgctgggccg cctggagcgc ttctgggtcg accagatcct gtacaagctc
     2461 taggccacgg cggttggtcg agcgagcctc ttgggatcgc tcgatccaga gcaaaaagaa
     2521 attgtggcgt cggggccaca ttggagcagc aaacgccggc cgtcgcgccg gcgtttctta
     2581 tttgcgccgc aaagtcctat agagactgcg gccttgcggt gcttttccca ggtctgcggc
     2641 gttcccgttt tccgcgtcgt ctccggcctc acgcctcgtt gtgttgtgaa gcacgggacg
     2701 cgaatggtat ttttgggccc tcggctcgcc ttgattggac tggtgactgc atgactttga
     2761 cc
//
LOCUS       DASZYN010000707         1669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_364939, whole genome shotgun sequence.
ACCESSION   DASZYN010000707 DASZYN010000000
VERSION     DASZYN010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364939"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="VHE33_18265"
     CDS             <1..1228
                     /locus_tag="VHE33_18265"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99454.1"
                     /translation="KQDPFMVERMHATEGKIIIDGNAACALGAMFAGVTVVTWYPITP
                     SSSVVEQLIDYLKKYRIEPDGKANFAVVQAEDELAAIGMVLGAGWAGARSMTATSGPG
                     ISLMAEFAGLGYFSELPGVIFDIQRVGPSTGLPTRTAQADLLSIAVLSHGDTKHPILL
                     PASVKECFEFAGAAFDLAEQLQTPVFVLSDLDLGMNNWMSDPFEYPDKPLNRGKVLTA
                     EDLKRLGEFARYKDVDGDAIPYRTLPGTDHPKASYFTRGSGHNEKALYSERPEDYQNL
                     MERLARKFDTARTMVPAPVVVQTGKSKVGIIAFGTSDFAVMESRDQLRKEYGVEADYL
                     RLRAYPFSKEVRDFVASHERVYVVEQNRDAQMLSLLKLDLPAAEITKLRSVRHFNGLP
                     IDARSVTDEIVLQEGI"
     gene            1310..>1669
                     /locus_tag="VHE33_18270"
     CDS             1310..>1669
                     /locus_tag="VHE33_18270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99455.1"
                     /translation="MAATATPAPKTNRIGLPVLEYRGSKTTLCAGCGHNAISERIIDA
                     MYEMGVQPERVMKLSGIGCSSKSPAYFMSRSHSFNSVHGRMPSVATGALLANKTMMGI
                     GVSGDGDSASIGIGQFVH"
BASE COUNT          330 a          584 c          451 g          304 t
ORIGIN      
        1 caaacaggac ccgttcatgg tcgagcgcat gcacgccacc gaaggcaaaa tcatcatcga
       61 cggcaatgcc gcctgcgccc tcggcgcgat gttcgccggc gtcacggtcg tcacctggta
      121 cccgattacg ccctcttcct cagtcgtcga gcagctcatt gactacctga agaagtaccg
      181 catcgagccg gacggtaaag ccaacttcgc cgtggttcag gctgaagacg aactcgccgc
      241 tatcggcatg gttctgggcg ccggctgggc tggtgcccgc tccatgacgg caacgtccgg
      301 tcctggcatc tcgctcatgg cagaattcgc cggtctcggc tatttctccg agcttcctgg
      361 cgtcatcttc gacatccagc gcgttggccc atcgacaggc ctgcccacgc gaaccgccca
      421 ggccgacctg ctttccatcg cggtcctctc ccacggagac accaagcacc ccatccttct
      481 tcccgccagc gtcaaagaat gcttcgagtt tgcaggcgct gccttcgatc tcgccgagca
      541 gctccagacg ccggtcttcg tcctctccga cctcgatctg ggcatgaata actggatgtc
      601 cgaccccttc gagtaccccg acaagccgct caatcgcggc aaggtgctga ctgccgagga
      661 cctcaagagg ctgggcgagt ttgcgcgtta caaagatgtg gatggcgacg cgatcccgta
      721 ccgcacgctc ccgggtaccg accatcccaa ggcctcctac ttcacccgcg gctccggcca
      781 caacgagaag gccctttact ccgaacgccc cgaagactac cagaacctca tggagcgact
      841 ggcacgcaaa ttcgataccg ctcgcaccat ggtgcccgcg cccgttgtcg tgcagaccgg
      901 caagtcgaag gtgggaatca tcgccttcgg cacctccgac ttcgcggtca tggagagtcg
      961 cgatcagctc aggaaggagt acggcgtcga ggccgattac ctgcggctcc gcgcctatcc
     1021 gttcagcaaa gaggttcgcg attttgtcgc atcgcacgag cgggtctatg tcgtggaaca
     1081 gaaccgcgac gcccagatgc tgagcctgct caagctcgat ctgcccgccg ccgaaatcac
     1141 caaactgcgc agcgtccgcc actttaacgg cctgcccatc gacgcgcgct ccgtcaccga
     1201 cgaaatcgtc ctgcaggaag ggatttgagg ccgcatcttg tacagctcca gcagacagtc
     1261 tcgatcaaca cagtccgtac gcaacaagtt cgttaagagg tagaaaagaa tggcggccac
     1321 cgctacacca gctcccaaaa ccaaccgcat cggactgccc gtcctcgagt atcgcggcag
     1381 caagaccacc ctctgcgccg gctgcggcca caatgccatc tccgagcgca tcatcgacgc
     1441 catgtacgaa atgggcgtgc agcccgagcg cgtgatgaag ctctccggca tcggttgctc
     1501 ctccaagagc ccggcctact tcatgtctcg ttcgcacagc ttcaacagcg tccacggccg
     1561 catgccctcc gttgccacgg gcgctctgct cgcgaacaag accatgatgg gcatcggcgt
     1621 ctcgggtgac ggcgactccg cctcgatcgg tatcggccag ttcgtccac
//
LOCUS       DASZYN010000708         2427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_556834, whole genome shotgun sequence.
ACCESSION   DASZYN010000708 DASZYN010000000
VERSION     DASZYN010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556834"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..894
                     /locus_tag="VHE33_18275"
     CDS             277..894
                     /locus_tag="VHE33_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99456.1"
                     /translation="MGRKIAIVALLLLPTALIAQSSESATGGEATVAAGVEFSTFNPD
                     WGCPTSAPFGCQTQLYGPTAVFNFDLHQKYGIEGEARWLHWHGNGGFVESNYLVGPRY
                     RLYRFSRMTLWGKLLMGGGWITTPNYPAAGSLKGSYFAYAPGATVNYRLTRMIALRAD
                     YEFQLWPSFAGPPTYDPSTGKVIQHQSGLTPNGVSVGVVYRFLGP"
     gene            complement(988..1938)
                     /locus_tag="VHE33_18280"
     CDS             complement(988..1938)
                     /locus_tag="VHE33_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HVT99457.1"
                     /translation="MVRKRSLLRKALWGLLAFAVLAACTEAALRWGLGLGNPILIAPD
                     PACSYVTRPNQNVYRFFVHTYINRYGMRSAEVSPERPPGVVRVLFVGDSVTYGTTHVD
                     QKNLFSQIVGRGLPSIVHEKVEVLNASVNGWAPDNEWEWVRTRGIFQSNYVLLVLNDG
                     DLTQPLAKIDQVGDSLPQTRPLTAINELFTRFIKPRILHIQPHQDPGDVAVPDAATER
                     ANLQDLDHFDDSVRNQQGQMAIVFLPFRADLPHPADKALGVLQTWTATHHVPLIDLST
                     AIAAHSVPEICIYDHLHFNTAGNAVIAQAILNQWPNALRP"
     gene            complement(2034..>2427)
                     /locus_tag="VHE33_18285"
     CDS             complement(2034..>2427)
                     /locus_tag="VHE33_18285"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HVT99458.1"
                     /translation="NYQYAQNVQRFCCSFGSYHIHDRFQEFSAGYVRSFYFRNWNPFI
                     EAGPGGFMFSPIDDSKTNTFNVSRSTNIGLMYGAGFAYEISPSFDIRAEYRGLVMKTP
                     TFSYPGDAFRTNVYYNIYDPVVGIAYHF"
BASE COUNT          512 a          672 c          774 g          469 t
ORIGIN      
        1 ctgcagcgtt attccgacgc acccgcggtt ccggcaaacg caacgccggc cgccagtgtg
       61 ccgccttcgg cagatgcggc ggctcagcag ccgtcggcaa gcgcgcaggc acggccgcag
      121 gccgggccac agtaatctgc acggaacaaa cgggcgcttc cggttcgcag tgtcaccgcc
      181 ggagcgccca tgcatgactc ggtacgagga ttcacgacaa aaacggcaag gttggaatcg
      241 gcaggtgcct gttagtcgca agaccggaga acgaacgtgg gcagaaagat cgcaattgtc
      301 gcactccttt tacttcctac agcactgatc gctcagagtt ctgaatcggc cacgggtggc
      361 gaagctacgg tggcagcggg ggtggagttt tccaccttta atccggactg gggctgccca
      421 acgagtgccc cgttcggttg ccaaacccag ctttacggcc cgacggcggt ctttaacttc
      481 gatcttcatc agaaatatgg gatcgaagga gaagcccggt ggctgcactg gcatggaaac
      541 ggcggcttcg tcgagtccaa ctatctggtc ggtccccgct atcgccttta ccggttcagc
      601 cgcatgactc tctggggcaa gctgctgatg ggaggcggct ggattaccac gccgaactat
      661 ccggcggccg gcagtttgaa gggcagctat tttgcgtacg cgccgggagc gacggtcaac
      721 tatcgcctga cgcgcatgat cgctcttcgc gccgattacg aattccagct ctggccttca
      781 ttcgccggac cccccactta cgatcccagt accgggaaag tgattcagca tcaaagcggg
      841 ctgacgccga acggggtgag tgtcggagtg gtctaccggt tcctggggcc ctaggaatcg
      901 cgagcacagt ttagaacaga gggctgggag caaatcccag cccttttcct ttgcaaccgg
      961 gaagcgcgtg aacccaatga gggccaatta gggtcgaagt gcgttcggcc actggttcag
     1021 gatcgcctgc gcgataaccg cattccccgc tgtgttgaag tgcaaatgat cgtagatgca
     1081 gatttccggg acagaatgtg cggcgattgc cgtgctcaag tcgatgagtg ggacatggtg
     1141 tgtggcggtc caggtctgca acacaccgag tgccttgtct gcaggatgcg gaaggtccgc
     1201 gcgaaagggc agaaagacga ttgccatctg accctgctgg tttcggactg aatcgtcgaa
     1261 atgatcaagg tcctgcagat tggcccgctc ggtcgcggcg tcaggaacgg caacgtcacc
     1321 ggggtcctgg tgcggttgaa tgtgcaggat tcgcggcttg atgaaacgcg tgaacagctc
     1381 attgatcgcg gtgagcggtc gagtctgcgg cagcgaatcg cccacctgat cgatcttcgc
     1441 cagaggttgg gtgaggtctc cgtcattcag cacgagcagg acgtaattgg actggaagat
     1501 gccgcgggtc cttacccact cccactcatt gtccggcgcc cagccattca ccgaggcatt
     1561 gagaacctcg accttctcgt gaacgatcga tggcagaccg cgcccgacga tttgcgagaa
     1621 caggtttttc tgatcgacat gggtcgtgcc ataggtgact gaatcgccga caaagagaac
     1681 gcggacgacc ccaggcggcc gttccggaga gacctccgcc gaacgcatcc cgtagcggtt
     1741 gatgtaggtg tgaacgaaga accgatagac attctggttg ggccgggtta cgtaagagca
     1801 ggccggatcg ggcgcaatga ggatgggatt ccccagcccg agtccccagc gcagtgcggc
     1861 ctcagtgcag gcagccagca cggcgaaagc cagaagaccc cagagcgcct ttcgcaggag
     1921 cgatctcttt cggaccaaga agagcgctcc gccactggca ttgccggttg tcatgaagaa
     1981 ggagtgtggg cggcggtgcc ggctgctgaa aggcgattcc gccgctgaat gttttagaag
     2041 tggtacgcga tcccgacgac cgggtcgtag atgttgtagt agacgttggt gcggaaggcg
     2101 tcgcccggat agctgaaggt cggagtcttc atcaccaggc ctcgatattc ggcgcggata
     2161 tcgaagctgg ggctgatctc gtaggcgaaa ccggcgccat acatcagacc gatgttcgtg
     2221 ctccggctga cgttgaaggt gtttgtcttg gagtcgtcaa tgggcgagaa catgaacccg
     2281 cccgggccag cctcgataaa gggattccag ttgcggaagt aaaagctgcg cacgtagccg
     2341 gcggagaact cctggaaccg gtcgtgaatg tggtacgaac cgaaggaaca gcaaaagcgc
     2401 tgaacgttct gggcatactg gtaattc
//
LOCUS       DASZYN010000709         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_620162, whole genome shotgun sequence.
ACCESSION   DASZYN010000709 DASZYN010000000
VERSION     DASZYN010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620162"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..456)
                     /locus_tag="VHE33_18290"
     CDS             complement(1..456)
                     /locus_tag="VHE33_18290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99459.1"
                     /translation="MSHIEEATTTLKFTSLAALMRQGDREAIEQHPCMALLRQAVTLV
                     ATAHHGTVRATYSNYSGDAQHTNTNLALHIPGKLPRGIGLVIDQQTGALSFRGDPWAH
                     EAFFAEVQQKIVQHYVVLAHAAALRRMHAQVSTQVLDDQQVVITGVLHG"
     gene            complement(637..>1503)
                     /locus_tag="VHE33_18295"
     CDS             complement(637..>1503)
                     /locus_tag="VHE33_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99460.1"
                     /translation="VEHLRTWGCTVSVDATGNVLAYRGAPAEGQGYPLLSFHLDCIWV
                     DPAPLEAPLSSEPKSTRRRRTRRSIPSLEPLPLEALILEDGWLHTNGRFVLGGDDKCG
                     AVIALTLAQMTDLPLKIVASVEEERGCVGIAQVDPAFFADVAYALVLDRRGANHLVVS
                     IADHLLCQGSFAAALMRAAADTGLLVYAAQGAFSDALTLSDAVPNVANLSVGYYHPHT
                     VHERVSVRDLWHAYRWVHAALHCLPCSRAETALPSEQEAPDPSCCARCHHLRLDPALV
                     PGDLHPLLCHCR"
BASE COUNT          319 a          470 c          470 g          244 t
ORIGIN      
        1 ttagccatgc agcacccccg taatcacgac ctgttgatca tcaagcacct gggtggatac
       61 ctgcgcgtgc atgcgccgca acgctgccgc atgggccagg accacatagt gctgcacaat
      121 cttttgctgc acctcggcaa agaacgcctc atgcgcccag ggatcacccc gaaaggacag
      181 ggctcctgtc tgctgatcga tgaccagccc gatgccgcgt ggaagtttgc cgggaatatg
      241 cagggccaga ttcgtgttgg tgtgctgcgc atcaccagaa taattgctat acgtggcacg
      301 aacggtacca tgatgggcgg tcgcgaccag cgtcaccgcc tgacgcaaga gcgccataca
      361 gggatgctgc tcaatggcct cacgatcccc ctgccgcatg agcgccgcca gggacgtgaa
      421 tttcagggtc gtcgtcgctt cttcaatatg actcatacca ttgctcctat cagaaacagg
      481 aaaacacaga acggtttgtc ctctcttcgg agaggacttc gcgtgggctc ctcagagaga
      541 tgggggtgcc cgcgaccaac acaccgagtg ggaggcaagc gtcatcgagg atgcctttct
      601 cccggcccag agggtgaagc cgggtgggaa gacgacttag cgacagtggc agaggagggg
      661 gtggaggtcg ccgggcacca acgccgggtc gagccgcagg tggtgacacc gcgcacagca
      721 cgaagggtca ggagcctcct gctcggatgg gagcgccgtc tctgctcgtg aacagggtaa
      781 gcagtggagc gctgcatgga cccagcggta cgcatgccac agatcccgca cggacacccg
      841 ctcgtgaacc gtgtgtgggt gataatatcc cacactcaga ttggccacat tgggcacagc
      901 gtcagaaagc gtcagcgcat cggagaatgc cccctgggcg gcgtagacca gcaggcccgt
      961 gtctgcggca gcccgcatca acgccgccgc gaaggaaccc tggcagagga gatggtcagc
     1021 gatggagaca accagatgat tggcccctcg ccggtccaac accagcgcat acgccacgtc
     1081 ggcaaaaaag gcaggatcga cctgcgcgat gcccacgcag ccccgttctt cttcgaccga
     1141 ggcgacgatc ttgagcggca gatccgtcat ctgcgccagg gtcagggcga tcaccgcgcc
     1201 acatttatca tcgccgccca gcacaaagcg tccgttggtg tgcagccatc cgtcctccag
     1261 gatgagcgcc tccagcggca ggggttccag cgatgggatg gatcgccgcg tccgccgccg
     1321 gcgtgtggac ttcggttccg aggaaagggg cgcctccagc ggtgccggat cgacccagat
     1381 acagtcgaga tgaaaggaga gcaagggata gccctgcccc tccgcaggag cgcctcgata
     1441 ggccaggacg ttccccgtcg catccaccga gacggtacag ccccaggtgc gcagatgctc
     1501 cac
//
LOCUS       DASZYN010000710         1682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1258360, whole genome shotgun sequence.
ACCESSION   DASZYN010000710 DASZYN010000000
VERSION     DASZYN010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258360"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VHE33_18300"
     CDS             <1..660
                     /locus_tag="VHE33_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99461.1"
                     /translation="FHDIRRPAEIERSDYAVSANIGRTITACRQAVVDVRCCLDWLES
                     QGYEEFGILGTSLGSCYAFIASAHDARIRINAFNHASMAFGDVVWTGQSTRHIRQGFE
                     QAGLTQDQSHRLWSAISPVSFMDRFAAFQKKVLVVYARYDLTFLPQYSEQVIENFARR
                     GIDFEVKVLPCGHYTTGETPYKFIDGWYLGSFVYRAFRDLPRRLAQVAEPAEAEKELV
                     P"
     gene            1113..1445
                     /locus_tag="VHE33_18305"
     CDS             1113..1445
                     /locus_tag="VHE33_18305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HVT99462.1"
                     /translation="MGKNQTNLLQGTLDLLILKAIGDGEMHGLGVSRRIDQITHGTFN
                     VKPGSLFPALHRMEEAGWLTSDWGESENNRRARFYRLTKAGHRQAGEEAAEWNRVALA
                     MTHALRSS"
     gene            1456..>1682
                     /locus_tag="VHE33_18310"
     CDS             1456..>1682
                     /locus_tag="VHE33_18310"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HVT99463.1"
                     /translation="MVLLGRIRAVVRNLIHRSKAEERLDAEARSYVDLLTEENIAAGL
                     APAEARRIALAQIGGLEQLKQSVRNERAGSG"
BASE COUNT          313 a          424 c          599 g          346 t
ORIGIN      
        1 ttccacgata ttcggcggcc agcggagatc gagcggtccg attatgcggt gagcgcgaat
       61 atcgggcgga cgattacggc gtgccggcag gcggtggtgg atgtgcggtg ctgccttgac
      121 tggctggagt cgcagggata cgaggagttt ggaatcctgg gaacgagtct ggggtcgtgc
      181 tacgcattta tcgcgagcgc gcacgatgcg cggatccgga tcaatgcctt caatcatgcg
      241 tcgatggcgt ttggcgatgt ggtgtggacg gggcagagca cgcggcatat ccggcaggga
      301 tttgagcagg cggggttgac gcaggaccag tcgcatcgac tgtggagcgc gatcagcccg
      361 gtgtcgttca tggatcggtt cgcggcgttc cagaaaaagg tgctggtggt ttatgcgcgc
      421 tacgatttga cgtttctgcc gcagtattcc gagcaggtga tcgagaactt cgcccggcgt
      481 ggcatcgatt ttgaggtgaa ggttctgccg tgcgggcact acacgacggg tgagactccg
      541 tacaagttca tcgatgggtg gtatctgggg tcgtttgtgt accgggcgtt tcgggatctg
      601 ccgcggcggc tggcgcaggt ggcggagccg gcggaagcag agaaggaact ggtgccgtga
      661 gttgctgctt gaagaccccg cggctgaagc cgctgctgtt ccgaccttga tttccgcagc
      721 ctgaaggctg cggcttctac cgtcgcctcg cacggcgagg ctgatgtgcg gtctatcgtc
      781 ggctgcggga cgtggctgga gatacctctt ccttgttacg cgaaccgctt cgtcccggat
      841 accgcgaatc tgggaacaga taatccctga tgtggtgttt ccgaaatttc tttatgcgga
      901 agagaagcag gtccctccgc ttccccttcg ctgcgctcag ggtccggtcg ggatgacaag
      961 gtaaaggaac ttcagaaaca ccacaccagg tcgttttggt gacaggcttc ccgcgtcatt
     1021 cgtgcatcgc acagtcgtca cgttcctatt gacattctgc aggagtgcag aggatagttg
     1081 attcctacag agaatcgata ggaaagctgg gcatggggaa gaatcagacg aatctgctgc
     1141 aggggacgct ggatctgctg atcctgaagg cgattggcga tggggagatg cacgggctgg
     1201 gcgtctcgcg gcgcatcgac cagatcacac atggaacgtt caacgtgaag ccggggtcct
     1261 tgttcccggc gctgcaccgc atggaagaag cggggtggct gacgtcggac tggggcgaat
     1321 cggagaacaa ccggcgggcg cgtttctatc gactgacgaa agcgggccat cgccaggctg
     1381 gggaagaggc ggcggagtgg aaccgggtgg cgctggcgat gacgcacgcg ctgcgatcga
     1441 gttaggagtc caggaatggt cttactgggc cgcattcgcg ctgtcgttcg caatctcatt
     1501 cacaggtcaa aggctgagga gcggctcgat gcggaggcgc gcagctatgt cgatctgctg
     1561 accgaggaga acatcgccgc tggtctggcc cctgccgaag cgcgccggat tgcactggct
     1621 cagatcggcg ggctggagca gctgaagcag tcggtgcgga atgagcgagc cggcagcggg
     1681 tt
//
LOCUS       DASZYN010000711         4348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_868340, whole genome shotgun sequence.
ACCESSION   DASZYN010000711 DASZYN010000000
VERSION     DASZYN010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868340"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VHE33_18315"
     CDS             complement(<1..838)
                     /locus_tag="VHE33_18315"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HVT99464.1"
                     /translation="MASTPLNLIRWMFDRDSGPGNRFAPRWIFLRALAGIYFSAFLAL
                     LFQIRGLIGPEGILPAGKLLEAIQRQSPGMLRFWYAPTLFWFSSSSHAMMVVVWAGLI
                     ASVLAFANVWPRVSFLVCWVCFLAFVAAAGDFSGYQSDGMLLEAGFLALFVAPNGWWP
                     GWGLDSPPSRVAFFLLQWEWFRIYFESGIVKLLSGDPQWRSLSAMDQYYQNGPLPTFV
                     GWYVQHLPHWFHVGTAAGTLVMELAIVWMLFLPRRVRVISFCIVTPWEIGVILTANYA
                     FLN"
     gene            complement(905..1369)
                     /locus_tag="VHE33_18320"
     CDS             complement(905..1369)
                     /locus_tag="VHE33_18320"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HVT99465.1"
                     /translation="MRYVIVVLAVAGIVVSALALHVHYSTGAEPCDINSHWDCGTVNR
                     SSYAVVPGIIWHLRAARHPDAMSGPAPRTGFPVATVGILGYLAIAIFALFRQRWLTLL
                     LAAGALGFALYLSHIEAHVLEVWCLYCVISQTLIAVIFLFCLVTIFVRPRSS"
     gene            complement(1444..2202)
                     /locus_tag="VHE33_18325"
     CDS             complement(1444..2202)
                     /locus_tag="VHE33_18325"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HVT99466.1"
                     /translation="MPATFTRHTVETERKDPGFGGKPPVDRRPTGGGGGDENWDSRHS
                     RRHGPREQLIRYRMAVFSILAADLVFFAVLVSAFFVRQSSVHIAPGDNVVSDWHPLSA
                     PPILWINTAILLLSAFTMEIARRQLFHEIDIMEEWLGLGRPAVRRAAPWLIATIGLGG
                     LFLAGQWMAWRQLASQGLYFATNPNSYFFYLVTGVHAVHLFLGLAALVFALAAMFRLR
                     RVEMRQIAVDCTAWYWHAMGVFWLFLFGLIVFFQ"
     gene            complement(2277..3932)
                     /locus_tag="VHE33_18330"
     CDS             complement(2277..3932)
                     /locus_tag="VHE33_18330"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HVT99467.1"
                     /translation="MKIVTTEPKALARLYLGLGLVAVVIGTLLSLLMRIHRTWPELAI
                     PLHGVIKPEDYLALVTMHGTLMVFFVLTVVPQSAFPNLVLPAQIGARGMALPRLNAAA
                     FWVTAASLLVLLAAFFVPEGAPIAGWTSYPPLSALAATGPGQATGMDLWLVAITLFCL
                     AAVLGSASILATILTRRAPGLTFRRLPLTVWSWLVSSGLTVIAFSALLLAATMLLSDR
                     HMGTSFFLPAGLVVNGIPQAAGTGSPMLWLHLFWFFGHPEVYIVVLPGMGLASSLLAN
                     FTRRPVPGYGLMVVSTLLIGVLGLGVWGHHIFVSGMNPYAGTAFGLTTMAIAIPSSAE
                     VLTWLAMTFRGRLQVNTPLLFVLGFISFFIAGGLTGPILAQPGLDAYLHNTFFVVGHF
                     HLVMSMAGVFSIFAGIYYWYPLLTGRRMNEVLGQIHFWVSLIAAYGTFFPMYQAGLAG
                     EPRHYAQLTGPVESFAKLIPVETGITWSAFLLGAAQLVFLFNLFWSARAKMAPSGANP
                     WRATTLEWSPGEHPTISTGPYCYGPEAGSDEDYRPQWASKKPF"
     gene            complement(3929..>4348)
                     /locus_tag="VHE33_18335"
     CDS             complement(3929..>4348)
                     /locus_tag="VHE33_18335"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99468.1"
                     /translation="GVQFQWYFRYPGRDGIFGVTRPSLVSAPAGNPLGLDPADPQAAD
                     DVVSSVMMLPAGQPVEITLRTQDVIHGFFVPGMRLKQDAIPGMTGHLEFTPETPGDYA
                     ILCSQVCGLGHYRMQARMRVVTREQFDAWLAAREHAQ"
BASE COUNT          998 a         1427 c         1305 g          618 t
ORIGIN      
        1 aattcaggaa cgcgtagttt gcggtgagga tgacgccgat ttcccacgga gtgacgatgc
       61 agaaggaaat cacccgcacc cggcgcggca ggaacagcat ccacacgatg gccagttcca
      121 tcaccagcgt gcccgcggca gttcccacgt ggaaccagtg cggcagatgt tgtacgtacc
      181 agccgacgaa ggttggcagc gggccattct ggtagtactg atccatcgcc gacaggctcc
      241 gccactgcgg atcgccggag agcagcttca cgatgcccga ctcgaaatag attcggaacc
      301 actcccactg gaggaggaag aaggcgacgc gcgaaggggg tgaatccagg ccccagcctg
      361 gccaccatcc gttgggcgcg acgaacaggg cgagaaagcc cgcttccagc aacattccgt
      421 cggactggta gccggaaaaa tcgcccgccg ccgccacgaa agccagaaag cacacccagc
      481 agaccaggaa cgagacgcgc ggccagacat tggcgaacgc cagcacggat gcgatcagcc
      541 cggcccagac cacaaccatc atggcgtgcg agctggaaga gaaccagaac agggtcggcg
      601 cataccagaa gcgcagcatc ccgggcgact gccgctgaat tgcctccagc aacttccctg
      661 cgggcaggat cccttcaggt ccaatcaatc cgcggatctg aaacaacagc gccaggaacg
      721 cagaaaagta gatgccggcc agcgcacgca gaaagatcca gcgcggggca aaacggttac
      781 cagggccgga gtcccgatcg aacatccacc ggatgaggtt cagaggcgta gatgccattt
      841 gtccgctcaa tcccgagcga cgaatcttct gggagttctc ctgcgatcat acggcgtcgg
      901 agtctcagga cgaacggggc cgaacaaaga tggtgacgag gcaaaacagg aaaatgaccg
      961 cgatcagggt ctgggagatc acgcaataga ggcaccagac ctccagaacg tgcgcctcga
     1021 tgtgactgag gtagagggcg aagcccaggg ccccggcggc cagcaaaagc gtcagccagc
     1081 gctgccggaa caacgcgaaa atggcgatgg ccagataccc cagaattccc acggtggcca
     1141 cgggaaatcc cgtccggggg gcgggcccgg acatggcgtc cggatgccgc gccgcgcgca
     1201 ggtgccagat aatccctggc accacagcgt aggagctgcg gttcaccgtc ccgcaatccc
     1261 aatgagaatt gatgtcgcag ggctcggccc cggtcgaata atgcacgtgc agcgcaagcg
     1321 cagacacaac gatgcccgca acagccagaa ctacgatcac ataacgcatg acttaacagg
     1381 gtacagccgt ggaggccgaa ccgggagttc gttcagcacc tctggcgggc cacaggctac
     1441 ggactactgg aagaaaacga tcaggccaaa caggaacagc cagaagacgc ccatggcgtg
     1501 ccagtaccat gccgtgcagt ccacggcgat ctgccgcatt tccacgcgcc gcagccgaaa
     1561 catcgcagcc agggcaaaca ccagagccgc cagcccaagg aacaggtgaa cggcgtggac
     1621 gccggtgacc agatagaaga agtagctgtt ggggttggtc gcgaaataga ggccctggga
     1681 agccagctgc cgccaggcca tccactgccc cgccaggaaa aggccgccca ggccgatcgt
     1741 cgcaatcagc cagggagccg cgcgccgcac cgccggccgt cccagcccca gccactcctc
     1801 catgatgtcg atctcatgaa aaagctggcg ccgggcgatt tccatcgtga acgcggaaag
     1861 cagcaggatg gccgtgttga tccacaggat cggcggcgcg ctcagcgggt gccagtccga
     1921 gacgacgtta tcgccggggg cgatgtgcac ggagctttgg cgcacgaaga atgcgctgac
     1981 cagcaccgca aagaagacca ggtccgctgc gaggatcgag aacaccgcca tccggtagcg
     2041 gatcagctgt tcccgcggtc catgccggcg cgaatgccgc gaatcccagt tctcgtcccc
     2101 tccgccgccg cccgtgggcc ggcgatcgac cggcggcttg ccgccaaatc ccggatcttt
     2161 ccgttccgtt tcgactgtgt gacgggtgaa cgtggcgggc atgggggctc cgtacgagtt
     2221 ctaaaagctt ataccggttt ggatgaggaa gaacacatcc tggacaggaa cggctgtcaa
     2281 aaaggttttt ttgacgccca ctgcggacga tagtcctcat ccgaaccggc ttccggtcca
     2341 tagcaataag gacccgtgct gatcgtggga tgctccccgg gcgaccactc gagcgtcgtc
     2401 gcccgccacg gattcgcgcc actcggcgcc atcttcgcgc gcgcgctcca gaaaaggttg
     2461 aaaaggaaca ccagctgcgc cgctccgagc agaaatgccg accaggtgat tccggtctcg
     2521 accggaatca gcttcgcgaa cgactccacg ggacctgtga gctgcgcata atgccgcggc
     2581 tccccggcca gccccgcctg atacatcggg aaaaatgtcc cgtaggcggc aatcagcgaa
     2641 acccagaagt gaatctgccc cagaacctca ttcatgcgcc gccccgtcag cagcggatac
     2701 cagtagtaga tccccgcaaa aatcgagaag actcctgcca tcgacatgac caggtggaag
     2761 tggcccacga caaagaaggt attgtgcaga tacgcgtcga gcccgggttg ggccagaatc
     2821 gggcccgtca gcccgccggc gatgaaaaaa gagatgaacc cgagcacgaa cagcagcggt
     2881 gtgttgacct gcagccgccc gcggaacgtc atcgccagcc acgtgagcac ctcggctgag
     2941 ctgggaatcg cgatcgccat cgtcgtgagc ccgaaagcgg ttccggcgta cggattcatg
     3001 ccggagacga agatgtggtg cccccataca cccagcccca gcacaccaat cagcagcgtc
     3061 gacacgacca tcagcccata cccggggacc ggtcgccggg tgaagttcgc cagcagcgaa
     3121 gacgcgagcc ccattcccgg cagcacgacg atgtagacct ccgggtggcc gaagaaccag
     3181 aacaggtgca gccacagcat cggcgaacct gttccagccg cctgtggaat cccgttcacg
     3241 accaggccag ccgggagaaa gaagctcgtt cccatgtgcc ggtcgctcag cagcatcgtc
     3301 gcggccaaca gcaatgcgct gaacgcgatt acggtcaggc cggaactcac cagccacgac
     3361 cacaccgtga gcggaagcct gcgaaaggtc agcccgggtg cacgccgggt cagaattgtc
     3421 gcgagaatac ttgccgaccc cagcaccgcc gcgagacaga acagcgtgat cgctaccagc
     3481 cacaggtcca tgcctgtggc ctgccccggc cccgttgcgg ctagtgcgct gagcggcgga
     3541 tagctcgtcc agcctgcaat cggcgcaccc tccggcacaa aaaacgccgc gagcagaacc
     3601 agcagggaag cggccgtgac ccagaatgcc gccgcattca gccgcggcag cgccatgccc
     3661 ctggcaccga tctgcgccgg caggaccaga ttcggaaacg cgctttgcgg gacgactgtg
     3721 aggacgaaga acaccatcag cgtcccatgc atcgtgacca gggccagata gtcctcgggc
     3781 ttgatgactc catgcagggg aatggccagc tccggccagg tccgatggat gcgcatcagc
     3841 agggacagca acgtgccaat gaccaccgcc accagtccca gacccagata cagcctcgcc
     3901 agcgccttcg gctctgtcgt cacgatcttc attgcgcatg ctcccgcgcc gccagccagg
     3961 cgtcgaactg ctccctggtc accacgcgca tgcgggcctg catccggtaa tgccccaatc
     4021 cgcatacctg gctgcacaga atcgcgtaat cgcctggtgt ttccggcgtg aactccagat
     4081 gccctgtcat ccccgggatc gcatcctgct tcagccgcat tcctggcaca aagaatccgt
     4141 ggatcacgtc ctgggtccgc agcgtgatct cgaccggctg ccccgccggc aacatcatta
     4201 ccgacgacac tacgtcatcg gccgcttgcg gatccgccgg atcgagcccc agaggattgc
     4261 ccgccggcgc gctgacgagg ctcggtctcg tcacgccaaa aatgccgtcg cgaccgggat
     4321 accggaaata ccactggaat tgcacacc
//
LOCUS       DASZYN010000712        10646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_172574, whole genome shotgun sequence.
ACCESSION   DASZYN010000712 DASZYN010000000
VERSION     DASZYN010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 10646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10646
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172574"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..1159)
                     /locus_tag="VHE33_18340"
     CDS             complement(218..1159)
                     /locus_tag="VHE33_18340"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HVT99469.1"
                     /translation="MPATRGHIPEKPGKTTWLHAAKKFPFRTIVAAVDFSEQSSAALR
                     CAQELARQQHAMLLLVHVIDPVGYAFPEGAPPAVDHDKTAKAELARIEAEVKKQGIPV
                     HSRVETGIVCERILLSLRDHKAALLVLGTKAVTGAGRAALGLVTRQLLAHSPCPILAV
                     APDDPKKPRKFGRWQNVLTAMDFSPASLSALHYAQRVTAAHLIVVHSARCGRHMECPN
                     CLERLRFLAPFDESHSVPVDHAVTGGDPARMIVQMAKKLHPDLIVMGAPSPAHTEHDL
                     DHSTVAQVIAAAPMPVLIVPEDRERYADELIEEVATA"
     gene            1758..4895
                     /locus_tag="VHE33_18345"
     CDS             1758..4895
                     /locus_tag="VHE33_18345"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HVT99470.1"
                     /translation="MNSAFAQSVGTGTITGTVTDKTQALVTTASVLVTNMDTGGSRTL
                     PVNGAGIYVAPFLQPGHYTVRVSAAGFATVEQAGLLLAVGQTLTVDASLPPASVSSQV
                     LVTSEAPVLDTQKTEVSQNLDATVISNLPVNGRRWDNFVLMTPNVAPDGNSGLISYRG
                     ISGLYNSNQVDGANNNQAFFSEARGRAIGSPYVYSQDSVQEFQSAVSGYSAEFGGAAG
                     GQINAVTKSGTNSIHGDLFYYLRYPSLNALDSYSKSQGLANHNPLLLTQPVHQQQQFG
                     GSVGGPIQRDKLFYFFTYDGFRKVNPIFYTSSVSGPTVLGYANSTTTCPTPLTTAQCT
                     AAAQYLVSLQNSYPRNIKQDIFFPKLDYQVNDANHVSVSFNWQNFHEPNGYNTAVQSN
                     NGSVTSNGTADFHSRFLVGTWSTAIRPTIANEVRFQWSRDLETDGTNSPGPAVSISGL
                     SGYGETSALPRAAFPDEHRIEFEDILSTTFGKHSLRSGADLNFIHEILINLFQGDGSY
                     SYSAGSAASSFAAWVQDVYSVNGGKHYTSFTQVNDPITHIGKDDFWNPDLAWFSSDTW
                     KVLSNLTVTLGLRYDLQLVPQPPKPNTTSALATYYTDTININHHEIQPRIGFAWSPLH
                     NTVVRGGYGLFYGLTSNSTYYTMRVENGVFQQQYNATPTTSWAPTAPNVLFQPPGPPL
                     AAPFAGASTPEVVNTGASLAALAFRGLDPHYTNPYSHSFDLGVEQQLPGKMTVSIGYV
                     GNRGMRLPIFIDTNLAPATTSKTYDITSPTGATSGTVTVPFYTARVTTADAAILTGFS
                     VVNSWYHSMAFTFHKPFSHGLEVVANYTLAHATDGGQVSGVNGTFNGTDTPINPFNLK
                     EEYGRSDLDMRERFVGSLVYAPTFNISSQVGRYLANGWLISGGATEQTGFPLTAFMAN
                     YPSGAPDGGVSGAELSLFNSPTGGRAPHVPRNGFPGPGARNIDARVSRDLPIHEKVKF
                     QITGEAFNLVNHPNVLGVNSTAFSYAAAGSGVCAGHTNACIYPYPTQRFGATTSTSST
                     LYGARQLQFSAKLFF"
     gene            complement(4999..5523)
                     /locus_tag="VHE33_18350"
     CDS             complement(4999..5523)
                     /locus_tag="VHE33_18350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVT99471.1"
                     /translation="MSRQPGTYAVMKTSEGTIVARLFEKDAPKTVANFIDLAEGKREW
                     THPVSREKSTNKLYDGTIFHRVIPDFMIQGGDPAGNGMGGPGYKFEDETKGSPHRFDK
                     KGKLAMANAGPNTNGSQFFITVAGTEWLTGKHTIFGEVVEGQEIADKISKVSARSDKP
                     VKPVVLESVTIERV"
     gene            complement(5571..6317)
                     /locus_tag="VHE33_18355"
     CDS             complement(5571..6317)
                     /locus_tag="VHE33_18355"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HVT99472.1"
                     /translation="MRSAIVAAAFLMAGSVFAQQSTPDSPGQTQTPPSTPQSAPATSS
                     QALPDAPGAQEHPGPQPTGATVLFDTSMGQMTCRLYDKQAPVAVANFVGLATGTKEFT
                     DPATKQKTKRPYYDNTTFHRVIPGFMIQGGDPLGTGEGDPGFSFNDEFDPALNFDVPG
                     RLAMANSGPNTNGSQFFITEGPQPSLDQKYVIFGQCDDHSVSVAMAIARVNRDSNDKP
                     LEPVTLKKVTVIPAGQPVPAAPAGLQAAPQ"
     gene            complement(6387..6743)
                     /locus_tag="VHE33_18360"
     CDS             complement(6387..6743)
                     /locus_tag="VHE33_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99473.1"
                     /translation="MQAETKQTFRVIVSLICRVALLVIAVPVLLRHFPNYKLPYRFPT
                     DWVIITVCDALAVVAPLLRSRKLAFFAGLAAMAAHYYYRHFVPMGDLVYMGVAILFVL
                     QPSSERKVVKDRPKTR"
     gene            complement(6776..8086)
                     /locus_tag="VHE33_18365"
     CDS             complement(6776..8086)
                     /locus_tag="VHE33_18365"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HVT99474.1"
                     /translation="MQKFTATFAVGAAMAAAMATVPSLAQNVLTGQAAFTDHSKEHPG
                     VRRHLTTADLPAPYATKGVDNGAEMVERPRDAWPQAPSGFKVELYYTGLDQPRLIRTA
                     PNGDLFVALSYQNKIMVFRGVGADGKPKEVSTFADGLSQPFGIQFYPAKDPKWVYIGN
                     TDSVVRFPYHAGDLKASGPAQKLTDLPGGGRLRGGGHWTRDIAFSPDGKRMFVSVGSH
                     SNDDDSDTHPEEFHRADVLEFTPEGKFVKVYASGIRNCVGEAINPTTGELWCSTNERD
                     MLGDNLVPDYITHVQEGGFYGWPWYYMSSNGGVQDPRQEGKHPELKGKVITPDVLVNP
                     HFASLEMLFYEGSQFPAEYKGDGFAAEHGSWNREPRSGYEVIRVPMKNGHATGEYEDF
                     LTGFVRPDGKVWGRPVGVTVANDGSLFVTDDGSGSIWHVIYTGK"
     gene            complement(8138..9148)
                     /locus_tag="VHE33_18370"
     CDS             complement(8138..9148)
                     /locus_tag="VHE33_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99475.1"
                     /translation="MTHRRTKLSIAIVLVVLLLLAGAIYLRKEAPPEAARLLPESDGI
                     IYFNLRPLRTATHFDQHPVQHSPDYQHFIDATGIQFEHDLDEVAFAVHRMPNPNGPNG
                     ALAFSEVFIGHFDGHKLADYLTGIAQSQEPYAGHTIYNVPSEGRTVRVVVLGYDSVAV
                     SNTPSSEQIHSMIDRYRTAALPFSGSSLLSKYYSKLPLLSVAWGIGQIGQPLANGGAR
                     VLGLKLPLPPDSTFLAALTWIGKIHVRVTEIAPSDAAAADTTESLQTILVLVKSMANT
                     PGAGSYDSDARALINSIVVERHRNEAVLTGTIPTALLQRAMNHPGELKSLPVPSGGKA
                     ER"
     gene            9336..9411
                     /locus_tag="VHE33_18375"
     tRNA            9336..9411
                     /locus_tag="VHE33_18375"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9369..9371,aa:Thr,seq:tgt)
     gene            9451..9538
                     /locus_tag="VHE33_18380"
     tRNA            9451..9538
                     /locus_tag="VHE33_18380"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9485..9487,aa:Tyr,seq:gta)
     gene            9572..9805
                     /locus_tag="VHE33_18385"
     CDS             9572..9805
                     /locus_tag="VHE33_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99476.1"
                     /translation="MSARIFEGWPMRVADGKRKLIALAFLAVIAALAWYTMDAGKVRL
                     VVLILLGGFALRIALTAGTQRNDLEGEGRHQGS"
     gene            9817..9892
                     /locus_tag="VHE33_18390"
     tRNA            9817..9892
                     /locus_tag="VHE33_18390"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9850..9852,aa:Thr,seq:ggt)
     gene            9966..10040
                     /locus_tag="VHE33_18395"
     tRNA            9966..10040
                     /locus_tag="VHE33_18395"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9998..10000,aa:Gly,seq:tcc)
     gene            10156..10464
                     /locus_tag="VHE33_18400"
     CDS             10156..10464
                     /locus_tag="VHE33_18400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99477.1"
                     /translation="MYQQASIPVQDEKTYAGVRHAIDEAFAPAQAAVFLKSVGGARLR
                     VRQFESVLAHGLLGADARKSYAALGDSDRGQIREHYLRQVERVAPEIRQKFYRLYASY
                     "
BASE COUNT         2008 a         3261 c         3206 g         2171 t
ORIGIN      
        1 gtcgtgccgc tggcgcggca ccgcttcgcg ggaggcaccc gatcatccgg tcgaagccct
       61 ggttcttatg tgaaagagaa gcaggtccct ccgcttcgct gcgctttggt cgggatgaca
      121 agctgaggaa gcaggtccct ccacttcgct gcgcttcggt cgggatgaca ggctgaggaa
      181 gcaggtccct ccacttcgat cggggtgaca cgcgacacta ggccgtcgcg acctcctcga
      241 tcagttcgtc ggcgtagcgt tcgcgatcct ccggaacaat caggaccggc atgggagccg
      301 cagcgatcac ctgcgcgacc gtgctgtggt ccagatcgtg ctcggtgtgg gcgggggagg
      361 gagcgcccat cacgatcaga tccggatgca gcttcttcgc catctgcacg atcatccgcg
      421 ccggatcgcc gccggtgacg gcgtggtcca ccggcacgct gtgcgactca tcgaacgggg
      481 cgaggaaacg cagacgctcg aggcagttcg gacactccat atgccgtccg cagcgcgccg
      541 agtgcacaac gatcaggtgc gcggcggtga ctctctgtgc gtagtgcagc gcgctcagcg
      601 aggcaggaga aaaatccatc gcggtcagca cattttgcca gcgcccgaac ttgcgcggct
      661 ttttcgggtc gtccggcgcc acagccagaa tcgggcacgg cgagtgcgcc aacaactgcc
      721 gtgtgaccag tccgagagcg gcgcggcctg cgccggtgac ggcttttgtc cccagcacga
      781 ggagcgctgc cttgtgatcg cgcagagaaa ggaggatccg ttcgcagacg atgcccgtct
      841 ccacgcgcga gtgcacaggg atgccctgct tttttacttc cgcttcaatc cgtgccagtt
      901 ccgccttcgc agttttgtcg tggtccacgg ccggcggagc gccttcgggg aacgcatatc
      961 caacgggatc gatcacgtgg accagcaaca gcatggcgtg ctgctgccgg gccagctcct
     1021 gagcgcagcg cagagccgcg gaggactgct cgctgaaatc aaccgccgcc acgatcgttc
     1081 tgaacggaaa cttcttcgcg gcatgcagcc aggtggtctt gcccggtttc tccgggatgt
     1141 gtcccctcgt tgctggcatg gccgatgccc tcccttgtca cacactgggc tttccctgaa
     1201 acctacgctc cacgaacagg gggcatccgt gacaaaggtc acccgtttct gtgaccccta
     1261 acacacgcat tttcaaggaa gcagttgcgc gacgtttcgg acgaaactgc gcggagaatt
     1321 ttgtcgcatc cacagaaacg ggtatgtgca ggcgaactcc ctcgacgctg ggagggcctt
     1381 aacgtcgcaa gcgcagtgca ccggcgatgg tgcggctggg cacaaatcca cagaaggcgc
     1441 tctgcctgcg cttccatgca atcgcaacaa aaaaggtgca actgactgtt acagttgcgc
     1501 tggttgcggc cgcgcgctgc gcgacaattc accacatatt cgcagggttg gtcagaaagc
     1561 caggctatac tgaccgcaat tccatacaaa gttgtaagtt cctaaagctt tttttctttc
     1621 gaggccagcc caacatgatc agaaaaagcg acgccttcgg tgtctccgcg cccgtttctt
     1681 cccatcgccg caacctttca tccctgctgc tgactgcaat ttttcctctg ctgattctgt
     1741 tttgcgcatt cactgggatg aattcagcgt ttgcgcagtc ggtgggcacc ggcacgatca
     1801 ccggtacggt cacagacaag actcaggccc tcgtcaccac ggcttccgtg ctggtgacca
     1861 atatggatac cggggggagc cggacgcttc ccgtcaatgg cgccgggatt tacgtcgcgc
     1921 cgttcctgca gcccggccac tacacagtgc gggtatcggc ggcgggtttt gccacggtgg
     1981 agcaagccgg ccttcttctt gctgttggac aaacgctgac cgtggacgcc agccttcccc
     2041 ccgcgagtgt ctcctctcag gttctggtca ccagcgaggc gccggtcctc gacacgcaga
     2101 agaccgaagt ctcgcaaaac ctcgatgcta cggtgatctc caacctgccg gtcaatggcc
     2161 gccggtggga caacttcgtg ctcatgacgc ccaacgttgc acccgatggc aacagcggcc
     2221 tgatctcgta tcggggaatc tccggcctct ataacagcaa tcaggtggac ggggccaaca
     2281 acaaccaggc cttcttttca gaagcccgtg gccgagcgat tggttcgccc tacgtgtaca
     2341 gccaggattc tgtgcaggaa ttccagtctg cggtgtcggg ttacagtgcg gaattcgggg
     2401 gcgccgccgg tggccagatc aatgcggtca cgaagtcggg gaccaacagc atccacggcg
     2461 accttttcta ctacctgcgc tacccgtcgc tcaatgcgct ggactcctac agcaagtcgc
     2521 agggcctggc gaatcacaac cctcttctgc tcacccagcc ggtgcatcag caacagcagt
     2581 tcggcggcag cgtgggcggc ccgattcaga gagacaagct cttctacttc tttacctacg
     2641 atggtttccg caaggtcaac ccgattttct acacgtcatc ggtgtccggc cccacggtgc
     2701 tcggctacgc aaactccacc acgacctgcc ccacaccgct gaccacggcc caatgcacgg
     2761 cggcggctca gtacctcgtc agcctgcaga actcgtatcc gcgcaacatt aagcaggaca
     2821 tcttctttcc caagctcgac tatcaggtca acgacgccaa ccacgtgagc gtttcgttca
     2881 actggcagaa ttttcacgag ccgaacggct acaacacggc ggtccagtcc aataacggct
     2941 cggtcacttc gaacggtacg gcggatttcc atagccgctt cctggtcggc acatggagca
     3001 cggccattcg gccgaccatc gcgaatgagg tccgtttcca gtggtcacgc gatcttgaga
     3061 ccgacggcac caactcgccg gggccggcgg tgagcatctc cggcctctca gggtacggcg
     3121 aaaccagcgc tttgccgcgc gccgcgtttc ccgatgagca ccgcatcgag tttgaagata
     3181 ttctctccac caccttcgga aagcactcgc tgcgctccgg agccgacctg aacttcatcc
     3241 acgaaattct gatcaatctt ttccagggcg acggcagtta ctcctactct gccggcagcg
     3301 ctgccagctc ctttgcggcc tgggttcagg atgtctactc ggtgaacggc ggaaagcact
     3361 acacctcttt tacccaggtc aacgatccaa tcacgcacat cggcaaagac gatttctgga
     3421 atcctgacct ggcgtggttc tcttccgaca cctggaaagt tctgagcaat ctgaccgtca
     3481 ctctcggcct gcgttacgat ctccagcttg tgccgcagcc gcccaagccg aacacgacca
     3541 gcgctttggc gacctattac accgacacga tcaacatcaa tcatcacgag atccagcctc
     3601 gcatcgggtt cgcgtggtct ccgctccaca acacggtggt ccgcggcggg tacggtctct
     3661 tctatgggct gacctccaac agcacctact acaccatgcg ggtggagaac ggcgtctttc
     3721 agcagcagta caacgccacc cccacgacca gctgggcgcc gaccgctccg aatgtgctgt
     3781 tccagccacc gggtccgcct ctggccgcgc cttttgcggg agcctctacg cccgaggttg
     3841 tcaatacggg agccagtctc gctgcactcg ctttccgcgg ccttgatcct cattacacga
     3901 atccttactc gcattcgttc gatctgggcg tcgagcagca gcttccgggc aagatgacgg
     3961 tttccatcgg ttatgtcggc aatcgcggaa tgcggctgcc catcttcatc gatacaaacc
     4021 tcgccccggc gaccacgagc aagacgtatg acatcacttc gccgacgggc gctaccagcg
     4081 gcacggtgac ggtgccgttc tacaccgcac gtgtaaccac cgcggacgcc gcgatcctga
     4141 cgggattcag cgtggtgaat tcgtggtacc actccatggc gttcaccttc cacaagccgt
     4201 tctcgcatgg cctggaagtg gtggcgaact acaccctggc tcatgcgacc gacggtgggc
     4261 aggtgtcggg cgtgaatgga acattcaacg gtaccgatac accgatcaat ccgttcaacc
     4321 tgaaggagga gtatggccgc tccgacctcg acatgcgcga gcgttttgtg ggcagcctgg
     4381 tgtacgcacc cacctttaac atctcctcac aggttgggcg ctatcttgcc aatggctggt
     4441 tgatctcggg cggggccacc gaacagaccg gcttcccgct caccgccttt atggccaact
     4501 acccgtcggg cgcgccggat ggtggcgtca gcggcgcgga actgagcctg ttcaactcac
     4561 ccaccggtgg gcgcgcgccc catgtgcccc gcaatgggtt ccccggtcct ggcgcacgca
     4621 atatcgacgc gcgtgtcagc cgcgacctcc ccattcacga gaaggtcaaa ttccagatca
     4681 cgggcgaggc cttcaatctc gtcaaccacc cgaacgttct cggcgtcaat tcgacggcgt
     4741 tctcctatgc cgcggcggga tcgggcgttt gcgccggcca caccaacgcg tgcatctatc
     4801 cctacccaac gcagcgtttc ggagcgacca catccaccag cagcacgctg tatggagctc
     4861 gccagctgca gttttccgcc aagctgttct tctaagaccg cgatttcgcc acaacagaaa
     4921 acccctgcct gcgggcaggg gtttgttttt tgcagttgag gagaggttcg acgttcccct
     4981 tcagtttcgt ggcttgcgct agacccgctc gatcgtcacc gactcaagca ccacgggctt
     5041 gaccggcttg tcggagcgcg ccgagacctt ggaaattttg tccgcgatct cctggccttc
     5101 gacgacttcg ccgaagatgg tgtgctttcc ggtgagccac tccgtgcccg ctaccgtgat
     5161 aaagaactgc gaaccgttcg tattcgggcc ggcattggcc atcgccagct tgcccttttt
     5221 gtcgaagcgg tgcggagatc ctttcgtctc gtcttcaaac ttatagccag gcccgcccat
     5281 gccgttgccg gccgggtcgc cgccctggat catgaagtcg ggaatgacac ggtggaagat
     5341 cgttccgtcg tacagcttgt tggtgctctt ttctcgcgac acgggatgcg tccactcgcg
     5401 cttgccctcg gccagatcga tgaagtttgc tacggtcttc ggcgcatcct tctcaaataa
     5461 ccgggccacg atcgtgcctt cgctggtctt catcactgca tacgtaccgg gctgacgcga
     5521 cataacaact ccttctctcg ttttgtggga tttttgatga atctgaaaac ttattggggt
     5581 gcggcctgca aacccgcagg cgccgcaggg actggctggc cggccggaat caccgtgact
     5641 ttcttcagcg tcacgggctc gagcggcttg tcgttgctgt cgcgattcac gcgcgcaatg
     5701 gccatcgcga cacttacgct gtgatcgtca cactgcccga agatcacata cttctgatcg
     5761 agagacggct gtgggccttc ggtgatgaag aactgcgacc cgtttgtgtt gggcccggaa
     5821 ttcgccatag cgaggcgtcc cggtacgtcg aagttcagcg cggggtcgaa ttcatcgttg
     5881 aagctgaagc cgggatcgcc ctcgcccgtg cccaacgggt ccccgccctg gatcatgaag
     5941 ccgggaatga cgcgatggaa ggtggtgttg tcgtagtagg ggcgctttgt cttttgtttg
     6001 gtggccgggt cggtgaattc cttcgttccg gttgcgagtc ccacgaagtt cgcaacggcg
     6061 acgggagcct gcttgtcgta gagcctgcag gtcatctggc ccatgctggt gtcgaacagg
     6121 acggttgcac cggtgggctg cggtcccgga tgctcctgcg cgccgggcgc atcgggcaaa
     6181 gcctgcgagg aggtggcggg agctgattgc ggcgtggatg gaggagtctg ggtctggccg
     6241 ggcgagtccg gcgtggactg ctgtgcgaag accgagccgg ccatcaggaa cgcggctgcg
     6301 acgatagcgc tgcgcattgg ggagctcaca gccaccagac tatcgcaatg cggggacctg
     6361 gtgggttgcg agcggtgcct gaggggtcat cgcgtttttg gcctgtcctt tacgaccttg
     6421 cgctcgctgg atggctgcag cacgaaaaga atggccacgc ccatgtagac caggtcgccc
     6481 atgggcacga aatggcggta gtagtagtgc gccgccatgg ccgcgagccc cgcgaagaac
     6541 gccagcttcc gcgagcgcag gagcggcgcc accacggcca gggcgtcgca cacggtgatg
     6601 atgacccagt cggttggaaa ccgatagggc agcttgtaat tcgggaaatg ccgcaggagc
     6661 acgggtacag caatgaccag cagagccacc cggcagatga gactgacgat gacccgaaag
     6721 gtttgcttcg tttctgcctg caccgatcca gcctacgata actgcgctcg cgcttttatt
     6781 tgccggtata aatcacgtgc cagatcgaac cagacccgtc atcagtgacg aacagggaac
     6841 catcgttcgc cacggtgacg cctacaggcc gtccccatac ttttccatcg ggccggacaa
     6901 atccggtgag gaaatcctcg tactcgccgg tggcatggcc attcttcatc ggcacgcgaa
     6961 tgacttcata tccggagcgc ggctcccggt tccacgatcc gtgctccgct gcaaatccgt
     7021 cgcctttgta ttccgccgga aattgcgagc cttcatagaa cagcatctcc aatgaagcga
     7081 aatgcggatt gaccagcaca tccggagtga tcactttgcc cttcagttcc ggatgcttgc
     7141 cctcctggcg aggatcctgc acgccgccgt tgctgctcat gtagtaccaa ggccagccgt
     7201 agaagccgcc ttcctgcacg tgcgtgatgt agtccggcac gagattgtcg ccgagcatgt
     7261 cccgctcgtt cgtggagcac cacagctcgc cggttgtggg attgatcgct tcaccgacgc
     7321 agttgcggat gcccgatgca tagaccttga cgaacttgcc ttccggcgtg aactccagca
     7381 cgtcagcgcg atggaactcc tcgggatggg tgtcggagtc gtcgtcgttg gagtgggagc
     7441 cgaccgagac gaacatccgc ttcccgtcgg gggagaaagc gatgtcacgg gtccagtggc
     7501 cgcctccacg aagacgcccg ccgccgggca ggtcggtcag tttctgggcg gggccgctcg
     7561 ctttcaggtc gccggcgtga tacgggaagc gcaccacgga gtctgtgttt ccgatgtaga
     7621 cccatttcgg atctttcgcc gggtagaact gaatgccgaa cggctggcta agaccgtcgg
     7681 caaaggtcga tacttctttc ggcttgccgt cggctccgac gccacggaac accatgatct
     7741 tgttctgata gctgagggcg acgaacagat cgccgttggg cgcggtacgg atcaggcgcg
     7801 gctgatcgag accggtgtaa tacagctcaa ccttgaagcc ggaaggggcc tgcggccagg
     7861 catccctggg gcgctccacc atctctgcgc cgttgtctac gcctttggtc gcatagggag
     7921 cgggcaggtc ggccgtagtc aaatgccgcc ggactccggg atgctctttg ctgtgatctg
     7981 taaaggcagc ctgcccggtg aggacgttct gggccaacga gggaaccgtt gccattgctg
     8041 ctgccatcgc cgcgccaacg gcaaacgtcg ccgtgaactt ctgcattgtt cctccagagg
     8101 gttggggaat tctgggacct tcgcgcctgc ttccagctta ccgctccgcc ttccctccac
     8161 tcgggacagg cagggatttg agttcgccag gatgattcat cgcacgctgc aacagagcgg
     8221 tgggaatggt tccggtgagg accgcctcat tgcggtggcg ctcgacgacg atgctgttaa
     8281 tcagggcacg cgcgtcggag tcatacgaac ctgcgccagg cgtgttggcc atcgatttca
     8341 ccagcaccag gatggtttgc agcgactccg tcgtgtccgc ggccgctgca tccgagggcg
     8401 cgatctctgt cactcgaacg tgaattttgc cgatccaggt gagggctgcg aggaatgtcg
     8461 agtccggcgg cagagggagt ttcagcccca gcacacgcgc cccgccattg gccaaaggct
     8521 gcccgatttg tccaattccc caggcgacgg agagaagcgg aagtttcgaa tagtacttcg
     8581 agagcagcga ggacccggag aacggcagcg ccgcggtgcg gtaccgatcg atcatggagt
     8641 gaatctgctc gctggagggt gtgttgctca cggccacgct gtcgtagccc aggacaacaa
     8701 cgcgaacggt acggccctcg ctggggacgt tgtagatggt gtggcctgcg tacggctcct
     8761 gcgattgtgc gatgcccgtg agatagtcag ccagcttgtg cccgtcaaag tgcccgatga
     8821 agacctcgct gaaagccaga gcgccgttcg ggccgttcgg gttcggcatg cgatgcacgg
     8881 cgaaggcgac ctcatccaga tcgtgctcga actggatacc ggtcgcatcg atgaaatgct
     8941 ggtaatcggg cgaatgctgg acgggatgct gatcgaagtg cgtagcggtg cgcagagggc
     9001 gcagattgaa gtagataatc ccgtccgatt cgggcagcag gcgcgctgcc tccggcggag
     9061 cttctttgcg aaggtaaatc gccccagcta acagcagcag gacaacaagc acaatggcga
     9121 tggacagctt cgtgcgccga tgggtcatcg agagggcccc ccggtgttcc gggaggcagc
     9181 gctatccatc ttctcatttg ccgccttttc cggcacgtcg ggcagcgtcg ccggtgtgat
     9241 tgccgcgcct ctttgcgtgt gatattttca atcctggacc ttccgcaaat ggacaccgtg
     9301 gtgtgcctca ggacgggggt tcgagcaagt tccgggctgg cgtagctcag ctggtagagc
     9361 atctgatttg taatcagagg gtcgggggtt caaatccctt cgccagctcc agttcttccg
     9421 gcctcggacc gatgagggtc ggatcccttc gcacaggtgg ccgagtggtt aatggcagca
     9481 gactgtaaat ctgccgctct ttggagctac ggaggttcga atcctcccct gtgcaccagg
     9541 attttgcgcc caccaggatt ggaacgcgcc ggtgagcgcg aggattttcg agggttggcc
     9601 gatgcgagtt gcggatggaa agcgcaaact gatagcgctt gcgttcctcg ccgtaatcgc
     9661 cgctctcgcg tggtacacga tggacgccgg gaaggtccgg ctggtggttt tgattttgct
     9721 gggaggtttc gctcttcgca ttgccctcac cgccggtact cagcggaacg atttagaggg
     9781 agaggggcgg caccagggtt cgtagttgct gtaaaggctg atgtagctca gttggtagag
     9841 cactcccttg gtaagggaga ggtcaccggt tcaatcccgg tcatcagctc cagattttga
     9901 gcagtaccca atacagcgtt cagcgatcag agttttccac ctgagagctg aacgctgcgc
     9961 agtgtgcggg agtaactcag tggtagagtc acagccttcc aagctgttgg tcgcgggttc
    10021 gattcccgtc tcccgctcca aaaactcgaa gaaggtggcg tcttgaaagg gcacggattc
    10081 agctttcccg atccatcgca aaatccaggg gccccggtcc ctgcaatcag tccctcggag
    10141 ggaagtcacc caacgatgta ccagcaggcc agcatccccg ttcaggacga gaagacctat
    10201 gccggcgtgc ggcacgcgat tgacgaggcc ttcgcccctg cgcaggccgc agtatttttg
    10261 aagagcgtgg gaggggcgag gctgcgggtc cggcagttcg agtcggtgct ggcccacggg
    10321 ctgcttggcg ccgacgcccg gaagagctat gccgctctcg gcgattccga ccgcggccag
    10381 attcgcgaac attacctgcg ccaggtggag cgcgtcgccc ccgaaatccg ccagaagttc
    10441 taccggcttt acgcgagcta ctgagtccgg agtaagttcc agatcatttt tgagaggaaa
    10501 cacgcatggc gaaggaaaag tttgaccggt cgaagccgca cgtgaacgtg gggacgattg
    10561 ggcacatcga tcacggcaag acgacgttga cggcggcgat cacgaaggtg ctgtcgaagc
    10621 acaatccgaa gattgcgttt cgctcg
//
LOCUS       DASZYN010000713         2641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_297832, whole genome shotgun sequence.
ACCESSION   DASZYN010000713 DASZYN010000000
VERSION     DASZYN010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297832"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VHE33_18405"
     CDS             <1..865
                     /locus_tag="VHE33_18405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT99478.1"
                     /translation="VDDGSTDNTAAVASAHGATVLSSAPLPVGWNGKTWACAQGAHQA
                     IGELLLFLDADTWFLPGGLDRLIARWQRAQDPNLILSLLPWHAITAMYEQLSLFFNLL
                     MASAGFAIFARPRLFGQSLLVTRQMYNAAGGHAVVRGAVLENLVLADCFREAGARLVC
                     LAGAGTLHMRMFPEGPHQMRESWVKGFAQGAANSEGLVIAWSIMWISGLWSTVALLLS
                     PIDYGRIELLLVYLLLAAQLAWLSRRVGNFSLLTCLLYPVPLVYFCSVFTVSVVRRSL
                     GYTNTWRGREV"
     gene            862..1341
                     /locus_tag="VHE33_18410"
     CDS             862..1341
                     /locus_tag="VHE33_18410"
                     /inference="COORDINATES: protein motif:HMM:NF040142.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99479.1"
                     /translation="MSALVWGANLLGWPAIHLSISSLFFRLPSQGFDKDCWLTTARRW
                     ELDGRLYRNGFGIRRWKAFLPDGAPWLGGFPKKHLHSHDPAYLNRFLLETRRAELAHW
                     SMLCCLPVFFLWNPPWACWVMTAYALAANLPCIVAQRYNRLVLRRLLLMESRGQARP"
     gene            complement(1395..1616)
                     /locus_tag="VHE33_18415"
     CDS             complement(1395..1616)
                     /locus_tag="VHE33_18415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99480.1"
                     /translation="MEQVEKALRALNIVEDMTYIRSVRKNRIDGSNQFILIFEEPLAG
                     RRARSPARCPELDAKPVVAIFGVLAAREE"
     gene            complement(1879..1970)
                     /locus_tag="VHE33_18420"
     tRNA            complement(1879..1970)
                     /locus_tag="VHE33_18420"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1932..1934),aa:Ser,seq:cga)
BASE COUNT          487 a          818 c          753 g          583 t
ORIGIN      
        1 ggtcgacgac ggatccaccg acaacactgc ggccgtagct tcggctcacg gtgcgactgt
       61 cctgtcctct gcgccgctgc cagtcggctg gaatggcaag acctgggcgt gcgcgcaggg
      121 cgcccaccag gcgatcggag aactgcttct ttttttagac gccgacacgt ggttcttgcc
      181 tgggggcctg gatcgcctca tcgcccggtg gcagcgggcg caggacccaa acctcattct
      241 ctcgctcctg ccctggcatg ccatcactgc aatgtacgag cagctttcgc tctttttcaa
      301 tctgctcatg gcttccgccg ggtttgccat atttgcgcgt ccgcgcctct tcgggcaatc
      361 actgctcgtg acccggcaga tgtacaacgc cgcgggagga cacgcggtgg tccgtggtgc
      421 ggttcttgaa aaccttgtcc tcgcggattg cttccgagaa gccggcgcac gactggtttg
      481 tcttgctggc gccggcacgc tgcatatgcg gatgtttccg gagggcccgc atcagatgcg
      541 cgagagttgg gtgaaagggt tcgcgcaggg cgctgccaac tccgagggtc tggttattgc
      601 ctggtcgatc atgtggatct cgggcctctg gtcgaccgtt gcgctgctgc tgtccccaat
      661 cgattacggc cgtatcgaac tgctcctcgt ctatctgttg ctggccgcgc agttggcgtg
      721 gctcagccgc cgcgtcggca acttcagcct cctgacctgc cttttgtatc cggtgcctct
      781 ggtctatttt tgttcggtct ttactgtctc cgtagtacgt cgctccctcg gttatacgaa
      841 cacatggcgc ggacgtgagg tatgagtgcg ctggtctggg gggcaaatct gctcggctgg
      901 cccgcgatcc atctgtccat ctccagcctc ttctttcgcc tcccttcaca agggttcgac
      961 aaggattgct ggttaacgac cgcgcgccga tgggaacttg atggccgtct ctatcgcaat
     1021 ggcttcggga ttcgcagatg gaaagcgttc ctgcccgacg gcgcaccctg gttgggcggc
     1081 ttcccgaaga aacacctcca tagccacgat cccgcatacc tgaaccgatt cctgctggaa
     1141 acccggagag cagaactcgc gcactggtcc atgctctgct gtttgcctgt cttcttcctg
     1201 tggaacccgc cctgggcatg ctgggtgatg actgcgtacg ctctcgcggc caacctgccc
     1261 tgcattgttg cgcagcgata caaccggctg gtacttcggc gtctcctgct gatggaaagc
     1321 cggggtcagg ccaggccatg accctgtcgg ggcggaggtc tcagccctcc ggtacgcgcc
     1381 gctatctcgt ccatctattc ctcacgcgcg gcgagaaccc cgaagatcgc cactacgggc
     1441 ttcgcatcca gctctggaca ccgcgcgggg cttctcgcgc gccgtcccgc gagcggctct
     1501 tcgaagatga gtatgaactg attcgagccg tcaatcctat tcttccgcac ggatctgatg
     1561 tacgtcatgt cctcaacgat attgagggcc cggagggctt tctctacctg ctccatttga
     1621 gttccgagca agccgccgct ttgggctggt gggattagcg atgccctatg gccgatgcgc
     1681 gtcggtatgc catggggtca gtctggtgcg ccaaaggcgc cggattgccg ttgtcatgcg
     1741 gggggtgctt tgtcatgcaa aaaagcccga agaagcggcc caaaggggag aaagcggtca
     1801 agccggacaa atcggcctgc catttaacct gcacagaatc cgcaggatac attgcaatta
     1861 ggaacttgcc aaaaattctg gcggagaggg agggattcga acccccgata gcctttcgac
     1921 tatgcctgat ttcgagtcag gtgcattcaa ccgggctctg ccacctctcc gcaacccgcg
     1981 gtgggaccgg cttttccgat tatcgcatct tctcgatcat cggtcctgcc cgctgacgtg
     2041 gcgcgcggtc cttcaacttc gcgatccttc cgctgcgctc cacaccccac gagcaaaagg
     2101 aaaggcctcc cgccggggag gcctaaaggg caatgcgcgt ttgtcagagg accggtatcc
     2161 ccggtcccaa ttgcgattct gctcgagcgc caatgacgcc gctatgaccc gaatcggtag
     2221 cagcatcagg acagttattg ttcccgcctc cacggtgctc ggccggccca gcgcggaccg
     2281 caaacgtcat cgggattctt cgtgccaaac actccgggac ccgccgcgtc tccccacgaa
     2341 cggagtgaac ttctgtgcaa ccgttatggt gcactggtca ctaaggtaac gcatgaagag
     2401 attaccctta ttggtaagaa ttgtccccct ttagggcaat tcctgccaac tgcaccttca
     2461 tcccgcgtca acaaaacggg actcttatcg atgaaaaggc tcataaatac gacatatcac
     2521 gccaaaaaca caaaggtaac ctgctgttga aaaggatggt gacccacgtg cctacacgag
     2581 gcacaggtta tccgcaaagt tcaaccttga accggggctg agcaagcagg accaaagaag
     2641 t
//
LOCUS       DASZYN010000714         2357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_365642, whole genome shotgun sequence.
ACCESSION   DASZYN010000714 DASZYN010000000
VERSION     DASZYN010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365642"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            607..1809
                     /gene="alaC"
                     /locus_tag="VHE33_18425"
     CDS             607..1809
                     /gene="alaC"
                     /locus_tag="VHE33_18425"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HVT99481.1"
                     /translation="MHEFQRIRRLPAYVFNITGELKAAARRRGEDIIDFGMGNPDGAT
                     PQHIVDKMIEAARRPETHRYSLSKGIPRLRRAITKWYANRYGIDLDPDTEAIVTIGSK
                     EGIAHLCLATLDQGDVVMVPNPSYPIHIYGPVIAGANIRSVAIHNADHFLAELEQAIP
                     LMYPRPKMLILNFPANPTTQCVDLSFFERVVALCRQHGIWLVHDLAYADIVFDGYRAP
                     SVLEVPGAKEIAVEFFTLSKSYNMPGWRVGFMVGNPELVSALARLKSYFDYGTFTPIQ
                     VAAIVALEGPQECVGEIRDNYQSRRDALVSGLNKAGWEVASPKATMFVWAPIPEPWRH
                     LGSLEFSTRLLNEAKVAVSPGIGFGEFGDGHVRFALIENEERTRQALRGIRQMLQREG
                     VSAAAAVR"
     gene            1873..>2357
                     /locus_tag="VHE33_18430"
     CDS             1873..>2357
                     /locus_tag="VHE33_18430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HVT99482.1"
                     /translation="MTPSFRDVRYIFLDRDGVINRKAPEGKYIAHWNELNMLPGVEAA
                     IAALNRSERHVIVVSNQRGIALGYYTGGDVEALHTRLQEHLALHGAHIDAFYYCPHDE
                     GECDCRKPGTGMFLQAFRDFPGASATNSLVIGDSISDIEAARELKMPSIFICGDPLTQ
                     KA"
BASE COUNT          476 a          664 c          727 g          490 t
ORIGIN      
        1 aacactacga tctgtctcct gccgtgcatg ggtccggcgg aacccgacat accgctttaa
       61 gagccttgcg gactggtgag gcctgagctg atagaagcac catacatcaa aacgcccggt
      121 tttgcattac cccggtaagg ccataagagc cccgatgacg cccccggcag gaggtctcca
      181 gtcctcctcg acgctgatcc tacgatgtct ccgctgccgt gtcgactgcc ctctggtaat
      241 gccgtcttct ttggtcgttg agccttcccg gtttggcaat ggggcaggaa aacccatgac
      301 tttggcgtta caaaagtctc agggaacggg ttcctggaga tccttgtcac gttacgttga
      361 gctggacgcg ttcggagcga agtttacgac tgcccagggg ctcgcgtccg gaccgtcgac
      421 gtccggttcc gtgaaacgac ttgtttcctg gggcagcatg gctcgacgcc gagagagcca
      481 gcgcagggaa gttcgatgaa agcaggaagg ctgacgtgat aacgaagaga cggggtgact
      541 ttgacccatt gcaatcctcc tggttctgaa cgactctgga actgaaccag tccacttgag
      601 gaacccatgc acgaatttca gcgaattcgt cgtcttccgg cctacgtctt caacatcacc
      661 ggagaactca aagcagccgc gcggcgccgc ggagaagaca tcatcgactt cggcatggga
      721 aaccctgacg gtgcgacgcc acagcatatc gtggacaaga tgatcgaggc tgcgcgccgc
      781 ccggagacgc accgctactc gctttcaaag ggcattccac ggctgcggcg cgcgatcacg
      841 aaatggtatg caaaccgcta cgggatcgat ctggatcccg acaccgaggc gatcgtgacc
      901 atcggatcca aggagggcat cgcgcacctg tgcctggcca cactggatca gggagatgtg
      961 gtcatggtgc ccaaccccag ctatcccatc cacatctatg gcccggtgat tgcgggtgca
     1021 aatatccgca gcgtcgccat ccacaatgcc gaccattttc tggctgagct ggagcaggcg
     1081 atcccgctca tgtatccgcg gccgaagatg ctgatcctga attttcccgc caatccaacg
     1141 acgcagtgtg tcgatctgag cttttttgaa cgggtcgtgg cactttgccg gcagcatgga
     1201 atctggctgg tgcacgatct ggcctatgcc gatattgttt tcgacggcta ccgggcgccg
     1261 tcggtgctgg aggtgccggg cgccaaagag atcgcagtgg agttcttcac gctttcgaag
     1321 agctacaaca tgcctggatg gcgggttgga ttcatggttg gaaacccgga actggtgtcg
     1381 gcgctcgccc gactgaagag ctatttcgac tacgggacct tcacaccgat ccaggtcgcg
     1441 gccatcgtgg ccctggaagg acctcaggag tgtgttggag agattcgcga taactatcag
     1501 agccgccgcg atgctttggt gagcggactc aacaaagccg gttgggaggt ggcttcgccg
     1561 aaagcgacga tgttcgtttg ggcgcccatt ccggaaccgt ggcggcatct tggctcgctg
     1621 gagttttcga cgcggttgtt gaacgaggca aaagtcgcgg tgagtcccgg gatcggtttc
     1681 ggtgaattcg gagacgggca tgtgcgcttt gcgctgatcg agaatgagga gcgcacgcgc
     1741 caggccctcc gcggaatccg ccagatgttg cagcgggagg gcgtgtcggc ggctgcggcg
     1801 gtgcggtgac gagcggagct ctatgatacg agtcaccatg atcatgattc cgtgcgactc
     1861 ggacttcctt cgatgacccc gagcttccgc gacgttcgtt atatctttct ggatcgcgat
     1921 ggtgtgatca accgtaaggc tccggaggga aaatacatcg cgcactggaa tgaactgaac
     1981 atgctgcctg gagtcgaggc ggccatcgcc gcgctgaatc gctccgaacg gcacgtgatt
     2041 gttgtcagca atcagcgagg gattgcgctg ggttattaca ccggcggcga tgtcgaggcg
     2101 ctccacacac gacttcagga gcatctcgcc ctgcacgggg cgcacatcga cgccttttac
     2161 tattgtccgc acgacgaggg cgaatgcgac tgccgcaagc ctggaaccgg gatgttcctc
     2221 caggcctttc gcgattttcc cggggcgtcg gcgacaaaca gtctcgtgat tggcgattcg
     2281 atttcggaca ttgaagcggc gagggagctg aagatgccgt cgatcttcat ctgcggagat
     2341 ccgttgacgc agaaggc
//
LOCUS       DASZYN010000715         4183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1137651, whole genome shotgun sequence.
ACCESSION   DASZYN010000715 DASZYN010000000
VERSION     DASZYN010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137651"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1327)
                     /locus_tag="VHE33_18435"
     CDS             complement(155..1327)
                     /locus_tag="VHE33_18435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT99483.1"
                     /translation="MSRFSGHKTAGEAVTVALLFAGFLYTGAMTVLLGLLLPRIAALH
                     GLSDRQAGALLATQAVCSATGALFVRHQFGRTLTAGYLLMAGGSAVLLIVPAWSDILA
                     IGAFGLGLGMAMTSTSIVVGRLFPDARGSALSLLNLSWSAGAAVCPLVVARIPGHFSA
                     MAVCIPIAACAALLALLIGPRTIRKVASVPQQESAVTGLSWTALLLFSALGFLYVGAE
                     STLGGWMTTYATRDVLWNFQKSNLAAGCFWGAMLVGRAVTPLFLRSVSERRLHLAAIV
                     AACLGTLVLVVARTPAALVTGASWTGFALAPIYPLTIALFLERAGESRNTGWVFAAAG
                     YGGALFPGLTGLVSTDMHSLSTGLLIPLAAATAMLPLALWLPKAPGASATRMAAQP"
     gene            complement(1314..2402)
                     /locus_tag="VHE33_18440"
     CDS             complement(1314..2402)
                     /locus_tag="VHE33_18440"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HVT99484.1"
                     /translation="MATRANGVKNGSKPLTLKTLAAYLNLDPATVSVVLNDVPGRSIP
                     EKTRQRIREAAQKFNYQPSFVARSLRSQRTMTIGILVPVLADGYHAEVMSGIGDYLLD
                     QNYFYFIAHHRHRTDLIERYPQMLASRGAEGIIAVDTHIPHQLTVPTVAVAGHHKHRG
                     LTNVVLDHARAGELTLRHLYDLGHRQIAFMRGQPFSSDSDVRWKSLVRVARDLGIRVR
                     PEMTIQLTRDLTSPELGYPVVQQLLACHRKFTALVCFNDIAAIGAIRALHDAQLRVPQ
                     DVSVIGFDDIPEAAYQMPSLTTIRQPLHQMGKLAAQLLLEHLHSRKPIPAEVAVEPEL
                     IVRETTAVVRASAESANGSRSKNSNVAV"
     gene            complement(2518..>4183)
                     /locus_tag="VHE33_18445"
     CDS             complement(2518..>4183)
                     /locus_tag="VHE33_18445"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HVT99485.1"
                     /translation="RPDTVLIGLDPSMTQFDLRDETPGYQGPGASRPILETPSGGTNI
                     VSGIGIYTNGINSRAVGVLWKAGPESLMDDVRFLGGHGTNAADGSRIDPYNNTHTADP
                     DLRRRWDGQYPSLWITDGGGGTFADIWTPSTFAQAGLYISNTSTPGHVYELSSEHHVR
                     NEAVLDGVKNWEIVALQTEEERGESGFALPLSIAHSSNITVANYHGYRVVSSFQPFPC
                     AIRISDSQEIHLRNVHVDSDSKVSFDNGVCDTTHHDEVRAREFANLDVPAEYSPAPQS
                     ADASAVLEPGAHVTQLASGFFNISGAAVDPHGQLYFVDARWQRIYHWKPETKEAEIVR
                     DSPLDPVNLAFDKGGDLLVVSYGGDGTVYSLRPDAPETQVTLLKPEPAAPRPAMQAIL
                     PVDAWSVHDLPRQPRWQYVAPDQSIFLPAGDDFVQGQLYYGTKMADVLRAFRLAPQEP
                     GHPFYISDESEEKTWKVTVSPSGSMDDLHLFAEAGGEGLAQDSAGQVWIAAGQILVYG
                     SDGNPLGRIDVPERPTGLVFGGPDRRTLYILTHTSLYAVRTKVPGL"
BASE COUNT          827 a         1264 c         1363 g          729 t
ORIGIN      
        1 caacactcca cacctgttcc ggtgccgcgc ctgctacggc agaacacgcg agaggatctt
       61 ccgcgacggg aagccgttgc tatccatata cgcccccggc ggcttttccg acgcagttgc
      121 gcagttctgg ctgattggga gtcccaggcg gcgttcaggg ctgggcggcc atcctcgtcg
      181 cagaggcgcc cggcgctttt ggaagccaca gcgcgagagg cagcatggcg gttgccgccg
      241 ccagcggaat cagcagcccc gtgctcagag aatgcatatc ggttgagacc agacccgtaa
      301 gaccaggaaa gagcgcaccc ccatagccgg ccgcggcaaa aacccagccg gtgttacggc
      361 tctctccagc ccgttcgaga aagagcgcga tggtcagggg gtaaatgggc gcgagggcga
      421 atccagtcca ggaggcgccc gtgaccagag ccgccggggt gcgcgccaca accagcacca
      481 acgtgcccag gcaggccgca acgatcgccg ccaggtggag acgccgctcg ctgaccgagc
      541 gcaggaacag cggagtcacg gcgcgtccca ccagcatcgc cccccagaaa cacccggcag
      601 caagattgct cttctgaaaa ttccagagca catcgcgggt ggcataggtg gtcatccagc
      661 cgcccagcgt ggattcggct ccgacataga ggaacccgag ggcagagaaa agcagaagcg
      721 ccgtccagga caagcctgtg acagcggatt cctgctgcgg gaccgaggcg accttgcgga
      781 tggtgcgcgg tccaatgagc agcgcaagca gagccgcgca tgcggcgatg ggaatacaga
      841 cggccatggc ggagaagtgc ccgggaatgc gcgcgactac caggggacac acagccgcgc
      901 cggcgctcca cgagagattg agcagcgaaa gcgcggaccc gcgtgcgtcc ggaaagagcc
      961 ggccgacaac gatactggtg gacgtcatgg ccatgccgag cccaagacca aaagcgccaa
     1021 tggcgagaat gtctgaccac gccggaacga tcagcaggac ggcgctgccg cctgccatca
     1081 gcagataccc ggcggtcagc gttcgcccaa attgatggcg gacgaagagt gcgcccgtgg
     1141 cgctgcacac agcctgggtt gcgaggagcg ctccggcctg tctgtcgctg agaccgtgca
     1201 gggcggcgat gcgaggcagc aggaggccca gaaggaccgt catggcgccg gtgtagagaa
     1261 acccggcaaa caggagcgcc acggtgacag cctcaccggc tgttttatgg ccgctaaacc
     1321 gcgacattgg aatttttgga acggcttccg tttgcggatt ccgcggaagc tcgcaccacg
     1381 gccgtcgttt cccggacgat cagttccggc tcaacggcta cttcggcggg gatcggcttg
     1441 cgcgagtgca ggtgctccag aagcagctgg gcggcaagct ttcccatctg gtgcagaggc
     1501 tgacgaatcg tcgtcagact cggcatctga taggccgctt cgggaatatc gtcgaatccg
     1561 atcacggaaa catcctgcgg cacccgcagt tgcgcatcgt gcagcgcgcg gatcgctcca
     1621 atggcagcaa tgtcgttgaa acagaccaga gcggtgaatt tccggtgaca cgcgagcagt
     1681 tgctgcacca ccggataacc cagttcaggc gaggtgagat cccgagtcag ctggatggtc
     1741 atctccggac ggacccgaat tccaagatcg cgcgcaaccc gcaccaggct cttccagcga
     1801 acatcggaat ccgagctgaa gggctggccg cgcatgaagg cgatttgccg gtgccccagg
     1861 tcgtagaggt gacgcagggt cagttccccg gctcgggcgt gatccagcac aacattggtc
     1921 agcccgcggt gcttgtggtg tccggcgacg gcaaccgtgg gaaccgtcag ctgatgcgga
     1981 atgtgcgtgt cgaccgcgat gatgccctcg gcgccccggc tggccagcat ctgcggatag
     2041 cgctcgatga ggtcggtgcg atgacgatga tgggcgatga agtagaagta gttctggtcc
     2101 agcaggtaat cgccgatgcc gctcatcact tcggcatggt agccatcggc gagaaccggc
     2161 acgagaatac cgatggtcat ggtccgctga ctgcgcaggg agcgggcgac aaagctgggc
     2221 tgatagttaa atttttgcgc ggcttctcgg atgcgctggc gcgtcttttc tggaatcgaa
     2281 cgcccgggaa catcgttcag aacgaccgag acggtcgccg gatcgaggtt gagatacgcc
     2341 gccagcgtct ttaaggttaa gggcttgctt ccattcttca ccccattcgc tctggtggcc
     2401 acgtgacgac aacacctcga tcgcagttga gttgaacttt tccctgaacc ttttcgggta
     2461 gcctgtcgac tgccaggcga tcgacccgat cagactctga gagactccgg ttcgccgtta
     2521 caaccccgga actttcgtcc tgaccgcata gagcgaagta tgcgtcagga tgtacagagt
     2581 ccggcgatcc ggtccgccaa acaccagacc ggtcggtctt tccggtacgt cgattctccc
     2641 cagggggttt ccgtctgaac catataccag aatttgtccg gcggctatcc aaacttgtcc
     2701 agcgctgtcc tgggcaagcc cctcgcctcc cgcctcggcg aaaaggtgca ggtcgtccat
     2761 cgatccggac ggagacaccg tgactttcca ggtcttctcc tccgactcat cgctgatgta
     2821 gaacgggtgg cctggctcct gcggcgccag ccggaacgca cgcaggacat cggccatttt
     2881 ggtgccgtag tagagctgac cctgcacgaa atcgtcgcct gcaggcagga aaatgctctg
     2941 gtccggcgca acatactgcc agcgcggctg cctgggcagg tcgtgcaccg accaggcgtc
     3001 cacgggaaga atcgcctgca ttgcgggccg gggcgccgca ggttcgggct tcagcagagt
     3061 gacctgggtt tcgggtgcat cgggccgcaa gctgtaaacc gtgccgtcgc caccataaga
     3121 aacgacgagc agatcgccgc ctttgtcgaa ggccagattg acggggtcga gagggctgtc
     3181 gcgaacaatc tcggcctcct tcgtctctgg tttccagtga taaatgcgct gccagcgcgc
     3241 gtcgacaaag tacagttgcc cgtgcggatc gaccgcggcg ccggagatat tgaagaaacc
     3301 agaggcgagc tgcgtcacgt gagcgccggg ttcgagcacc gcggacgcat cggcggactg
     3361 cggcgctgga gagtattcgg cgggcacgtc gaggttcgcg aattcgcgcg ctcgcacctc
     3421 gtcatgatgc gtggtgtcgc agacgccgtt gtcgaaggag actttgctgt cgctgtccac
     3481 atgaacgttg cggagatgga tctcctgcga atcggagatc cgaatggcgc agggaaaagg
     3541 ctgaaagctg ctgacgacgc gatagccgtg atagttggcg acggtgatgt tggaggagtg
     3601 ggcaatcgaa agaggcaaag caaagccgct ctcgccgcgc tcttcttcgg tctgcagcgc
     3661 aacgatctcc cagttcttca cgccgtcgag caccgcctcg ttgcgcacat ggtgctcgct
     3721 ggacagttcg tagacgtggc ctggcgtact ggtgttggag atgtagaggc ccgcctgagc
     3781 gaaggtgctc ggggtccaga tatcggcgaa ggtgccgccg ccgccatccg tgatccacag
     3841 gcttggatac tgcccatccc agcgccgccg cagatcggga tcggcggtat gcgtgttgtt
     3901 atagggatcg atgcgcgaac cgtcggctgc attcgtgccg tggccgccga ggaagcgaac
     3961 gtcatccatc agcgattccg ggccggcctt ccagagcacg ccgactgcgc ggctgttgat
     4021 gccgttggta tagatcccga tcccgctcac gatgttggtg ccgccgctcg gggtctcgag
     4081 gataggacgc gatgcgcctg ggccctggta tcccggcgtt tcgtcgcgca gatcgaactg
     4141 ggtcatgctg ggatcgagcc cgatcagcac cgtgtccgga cgc
//
LOCUS       DASZYN010000716         7772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1258848, whole genome shotgun sequence.
ACCESSION   DASZYN010000716 DASZYN010000000
VERSION     DASZYN010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258848"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1495)
                     /locus_tag="VHE33_18450"
     CDS             complement(104..1495)
                     /locus_tag="VHE33_18450"
                     /inference="COORDINATES: protein motif:HMM:NF014147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HVT99486.1"
                     /translation="MTASASLPAPLRAFTTGAAPASEVRAWRTSKDEEFKLIEAPVWE
                     SGLTASAMSIRLDPNTRCQEVLGFGAALTEASCSLLGQLDAEARRKILEDCFGPNGLR
                     LSVGRTTIGSSDYSLTAYSYDDSEQPDPQLTHFSIDHDLKYILPVLREARQVNREMFY
                     FSSPWSPPAWMKASHTLLGGAMRRHYFPAYAEYMRRFLEGYAKNGVTIDAITVQNETD
                     TEQDGNMPAALWGQEYEMEFVKAHLGPALEKAGLKTKIWILDHNYDLWGRAVDELSDP
                     EVSKYVDGVAWHGYVGEPSSMTRVHDMFPQKNAYWTEGGPDYTDPAYATDWSKWSSTF
                     TGILRNWARCIVSWNLVLDEKGRPDIGPFSCGGLVTLDSKTHAVTRSGMYKAFAQYSR
                     AMQRGARVIASWNSGGGELPGIAHVAAENPDGSKVMVLTNQQGGEQKMQVLLGDQAMA
                     VLLPPDSVTTVVW"
     gene            1714..5166
                     /locus_tag="VHE33_18455"
     CDS             1714..5166
                     /locus_tag="VHE33_18455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT99487.1"
                     /translation="MNNPDTTPAPAAPRARSDRAAAPDSTAHALFAGGGEVGALMAQV
                     DWSATPLGPVETWPQSLRTVVRIVLTSRFAMWMGWGPDLVFLYNDSYARMTLGKKHPW
                     ALGRPAREVWREIWDDIGPRIETVLHTGVATWDEALLLFLERSGYREETYHTFSYSPL
                     SGDDGRITGMLCVVSEETDRVIGERRLSFLRSLASALGQAIAEKDVLAAMETCLAQNQ
                     KDLPFTLTYLFGGDDLLHLASASGIPSSHPAAPTQLGPSDGPWPFDELLNGRNLILVD
                     DLPGLFPDLPTGAWTLPPSRAVIISLQRQGQDRPAGFFIAGLNPWRQVDMEYAGFIEL
                     IAGQIAASLANARAYEQERRRAESLAELDREKTIFFSNVSHEFRTPLTLMLGPLEDVL
                     SQRPPALPPQDLIQIETVHRNGLRLLKLVNTLLDFSRIEAGRIQALYLPVDLSARTAE
                     LASVFRSAMERAHLSYRIACEPLAEPVYVDLDMWEKIVLNLLSNAFKFTLQGEIEVTL
                     RSAAGHAELSVRDTGTGIPESELPRVFERFHRIEGAPGRTHEGTGIGLALVDELVRLH
                     GGTVQVDSQLGSGTTFTVRIPFGSAHLPPERIAPPDQSAANTLGAAPYVQEALRWLPG
                     AAATDEPLVKDLAYEELGVLLERKDQDKRARILLADDNRDMREYVQRLLSRKFSVTAV
                     ADGEEALASARENTPDLVLTDVMMPRMNGFELLRALRTDPATAAIPILMLSARAGEEA
                     ESEGLEAGADDYLVKPFTARELLARVSSHIAMQRLRQELTARERELRTKAEKAEAQYR
                     AILESISEGFVFVDRDWRVLYANRQVAAMAAVDPAFMIGRRLWDVVPEVTNTAFGRAF
                     REAMETNEVTRVEDFYPPSQRWIHASAYPSGDGLSLFGQDVTETRIQQEKLLLTEKLA
                     ATGRLAATIAHEINNPLESVLNLIYLARTSRAEIDRIREYLGTAEKELTRVAHIARHT
                     LGFYRETSLPTDIDLLALIDEVLTVYESRLRASSIEVRRDFTIVPTLRGLRGELHQVF
                     SNLISNSIDAMREGGTLTLSIRQPGEVRDRVVVAVQDTGMGIPQENIPRLFEPFFTTK
                     PSAGTGLGLWVVRQFVTSWGGNVEVQSETDSARHGTTFTLLLPLVAVSEKRAVPEQVS
                     ARLM"
     gene            5188..5640
                     /locus_tag="VHE33_18460"
     CDS             5188..5640
                     /locus_tag="VHE33_18460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT99488.1"
                     /translation="MLDRILWLQSAAPPPPPPHVSSDGEPARSRTTLRVLVVDDERLI
                     ADTVAVILSNSGFSAVAAYSGEQALEAARSQQPDIVLTDVLMPKMTGVELGIQIRSEF
                     PQVRVLLFSGQAATSELMRKAHDQGHDFELFPKPIHPEELIAKLKTRW"
     gene            complement(5673..5900)
                     /locus_tag="VHE33_18465"
     CDS             complement(5673..5900)
                     /locus_tag="VHE33_18465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99489.1"
                     /translation="MANANEQPSEAELARSLARLWPHREHESNAVMGLFCRCGLHRWR
                     KLDLANIVPGRAVDYCFWCSKIRIDGTLHDF"
     gene            complement(5907..7382)
                     /gene="gatB"
                     /locus_tag="VHE33_18470"
     CDS             complement(5907..7382)
                     /gene="gatB"
                     /locus_tag="VHE33_18470"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HVT99490.1"
                     /translation="MATAVTLSPEVLARYEAVIGLEVHVQLLTETKTFCGCANKFGAP
                     PNTNVCPVCLGLPGALPVLNQRAVEFATLASLALNCQVRERSIFARKNYFYPDLPKGY
                     QISQFDKPLGEHGWIEVPSGDSKKRIGITRLHMEEDAGKSLHDGFPDSATRTYVDLNR
                     CGTPLIEIVSEPDMRTPDEAFEYLTRLKEIIVYTGVSDGNMEEGSLRCDANVSVRPRG
                     QEKFGTKAEVKNVNSFRFIRAALEYEIERQIEIVESGGRVVQETRLYNASEGRTYPMR
                     SKEQAHDYRYFPEPDLPPLIVTEAWKAEIASHMPELPEARRARMVAEYGITEKDAQTL
                     TATRAFADQFEEAARAAKNPRRVANLVQSELTMRLKAADLELEQSPISMKGIALAADL
                     SEDGKLSSKQLKQLFDQCFERKEDFPAVYEREKPQQITDSSVIEKMIDEVIAANPAQV
                     EQYRKGKKTVAGFFVGQVMKASKGQANPALLNELVTKKLDS"
     gene            complement(7583..>7772)
                     /locus_tag="VHE33_18475"
     CDS             complement(7583..>7772)
                     /locus_tag="VHE33_18475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99491.1"
                     /translation="HWHIIRNGALLVGAESHTLLTDLKRIPRLSLSYAGAVVALTRKV
                     ASVLVRRDEVVVPAGAGD"
BASE COUNT         1386 a         2822 c         2093 g         1471 t
ORIGIN      
        1 gggccacgtc gaccgcgcga cgaagctgca aaccttacca gaggccctcg aatatttatg
       61 gcggggctat ccaatcaaat aggcactgga aaatctcgcc ccctcaccaa accaccgtcg
      121 tcaccgaatc cggcggcaac agcaccgcca tcgcctgatc ccccagcagc acctgcatct
      181 tctgctcgcc gccctgctga ttcgtcagca ccataacctt gctgccgtcg ggattctccg
      241 cagccacgtg cgcgatcccc ggcagctcgc cccctccgga attccacgaa gcgatcactc
      301 gcgccccacg ctgcatcgcc cgcgagtact gcgcaaaggc cttgtacatg ccgctcctcg
      361 tcaccgcatg cgtcttcgag tccagcgtca ccagcccgcc gcagctgaac ggtccaatgt
      421 ccggccgccc cttctcgtcc agcaccagat tccagctcac gatgcaccgc gcccagttgc
      481 gcaaaatgcc ggtaaaggtc gacgaccatt tgctccagtc cgtcgcatac gccggatcgg
      541 tgtagtccgg accgccctcc gtccagtacg cattcttctg cggaaacatg tcgtggacgc
      601 gcgtcatcga cgacggctcg cccacgtacc cgtgccacgc cacgccgtcg acatatttgc
      661 tgacctccgg atcgctcagc tcgtccaccg cgcgccccca caggtcgtag ttgtggtcga
      721 ggatccagat cttcgtcttg agtcccgcct tctccagagc cggtcccaga tgcgccttca
      781 cgaactccat ctcgtattcc tggccccaca gcgcggctgg catgttccca tcctgctccg
      841 tgtccgtctc gttctgcacc gtgatcgcat cgatggtcac gccgttcttc gcgtaaccct
      901 ccaggaatcg ccgcatgtac tccgcgtacg ccgggaagta gtgccggcgc attgcgccgc
      961 ccagcagcgt atggctcgcc ttcatccatg ccggcggact ccacggcgac gagaagtaaa
     1021 acatctcccg attcacctgg cgcgcctcgc gcagcaccgg cagaatgtac ttcagatcgt
     1081 gatcgatact gaagtgcgtt agctgcggat ccggctgttc gctgtcgtcg taactgtacg
     1141 cggtcagcga gtagtcgctg gaaccgatcg tcgtacgccc cacggaaagc cgcagcccgt
     1201 tcggtccgaa gcagtcctcc agaattttgc gccgggcttc agcatccagc tgtccaagca
     1261 aagaacaaga ggcttccgtc agcgccgcgc caaaccccag cacctcctgg cagcgtgtgt
     1321 tgggatcgag ccggatgctc atcgcgctgg ccgtcaagcc gctctcccac accggcgctt
     1381 cgatcagttt gaattcctca tccttcgaag tgcgccacgc ccgcacctcg ctcgcaggag
     1441 ccgcgccggt cgtgaacgcg cgcagcggcg ccggcaaact tgccgacgcc gtcattgccg
     1501 aaagcttcag aaaagtcctt cttgttgttc tggtcatgtc gcgcaacaga ataccgttcc
     1561 ccgccctacg cgaaaaccct tttccgcagc cggtggcaac tctgttccgt tcttcccgca
     1621 tctctgctgc gagtcatttg aatctgccaa aacattgccg ccccttccac gggaacggca
     1681 aacccattcg tgagcggtcc acgagattgc aacttgaaca atccggacac aacgcccgcc
     1741 cccgccgcgc cccgcgcccg atccgatcgc gctgcagcac cggattccac cgcccatgcc
     1801 ctcttcgccg gcggtggtga agtcggcgcg ctcatggccc aggtcgactg gtccgccaca
     1861 cccctcggtc cggtcgaaac ctggccgcaa agtctccgta ccgtcgtccg cattgtgctc
     1921 acctcgcgct ttgccatgtg gatgggctgg ggccccgacc tggtcttcct ctataacgac
     1981 tcctacgcgc gcatgaccct cggcaagaag cacccctggg ccctgggccg ccccgcgcgc
     2041 gaggtctggc gcgaaatctg ggacgacatc ggtccccgca tcgaaaccgt tctccacacc
     2101 ggcgtcgcga cctgggacga ggccctgctc ctcttccttg agcgcagcgg ctatcgcgaa
     2161 gagacctacc acaccttctc ttacagcccg ctgagcggcg acgacggccg catcaccggc
     2221 atgctctgcg tggtcagcga agaaaccgac cgcgtcatcg gcgagcgccg tctcagcttt
     2281 cttcgctctc tggcctccgc cctcggccag gccatcgccg aaaaagacgt gctcgccgcc
     2341 atggaaacct gtctggcgca aaaccagaaa gatctccctt ttaccctcac ctacctcttc
     2401 ggcggtgatg accttctcca cctcgcctcg gccagcggaa ttccctccag ccatcctgcg
     2461 gctcccacgc aacttggccc ttccgatggc ccatggccct tcgacgaact cctcaatggc
     2521 aggaatctca tcctcgtcga cgatcttccg gggcttttcc cggaccttcc caccggcgcc
     2581 tggacccttc cgccgtcccg cgccgtgatc atcagcctgc agcgtcaggg acaggatcgt
     2641 cccgccggct tcttcatcgc cgggctcaac ccctggcgac aggtcgatat ggaatacgcc
     2701 ggattcatcg aactcatcgc cggccagatc gccgccagcc tcgccaacgc ccgcgcctac
     2761 gagcaggaac gccgccgcgc cgaatctctt gccgagctgg accgcgaaaa aaccatcttc
     2821 ttctccaacg tcagccacga gttccgcact ccgctcaccc tcatgctcgg cccgctggaa
     2881 gatgtcctga gccaacgtcc acccgccctg ccgccacagg atctgatcca gatcgaaacc
     2941 gttcaccgca acgggcttcg tctcctcaaa ctggtcaata ctctgctcga cttctcccgt
     3001 atcgaagccg gccgcatcca ggcgctctat cttcccgtcg atctcagcgc ccgcaccgcg
     3061 gagctggcca gcgtctttcg ctcggccatg gagcgcgccc acctctccta tcgcattgca
     3121 tgcgagccgc tggccgagcc ggtctacgtc gacctcgata tgtgggagaa gatcgtcctc
     3181 aaccttctct ccaacgcctt caagttcacg cttcagggcg agatcgaggt cacccttcgc
     3241 tccgcagccg ggcacgctga gctctcggtt cgcgataccg gcaccggcat tccggaatcc
     3301 gagctgcctc gcgttttcga gcgcttccat cgcattgaag gcgcgcccgg ccgcactcac
     3361 gaaggcaccg ggatcggcct cgccctggtc gacgagttgg tccgcctcca tggcggcacc
     3421 gtccaggtcg acagccagct gggctccggt acgaccttca ccgtccgcat ccccttcggc
     3481 tccgcgcacc tgccgccgga acgcattgcg ccgcccgacc agtccgcagc caatacactc
     3541 ggcgctgcgc cctacgtgca ggaggccctg cgctggcttc ccggcgcagc cgccactgac
     3601 gaacccctcg tgaaagacct cgcctacgag gaactcggcg tcctgctcga gcgcaaggac
     3661 caggacaagc gcgcccgtat cctgctcgct gacgacaacc gcgacatgcg cgaatatgtc
     3721 cagcgcctcc tcagccggaa attcagcgtc acggccgtcg ccgacggcga agaagccctc
     3781 gcctccgccc gcgaaaacac gccggatctc gtcctgacag acgtcatgat gccgcgcatg
     3841 aacggctttg agctgctgcg cgctctgcgc accgatcccg ccaccgccgc cattccgatc
     3901 cttatgctct cggcccgcgc cggcgaagag gccgaaagcg aaggactgga agccggcgcc
     3961 gacgactacc tcgtcaagcc ctttaccgcc cgcgagctcc tcgctcgcgt cagttcgcac
     4021 attgctatgc agcgcctccg ccaggagctc accgcccggg agcgcgaact gcgcaccaag
     4081 gccgaaaaag cggaggccca gtatcgcgcc atcctcgaaa gcatctccga gggcttcgtc
     4141 ttcgtcgacc gtgactggcg cgttctctat gcgaaccgcc aggtcgccgc gatggccgcc
     4201 gtcgatcccg ccttcatgat cggcaggcgt ctgtgggacg tcgttccgga agtcacgaat
     4261 accgcgttcg gacgcgcctt tcgcgaggcc atggaaacca acgaggtcac ccgcgtcgaa
     4321 gacttctatc cgccgtcgca gcgctggatc catgccagcg cctatccttc aggcgatggc
     4381 ctctccctct tcggccagga tgtcaccgag acgcgcatcc agcaggagaa gctcctgctt
     4441 accgaaaaac tcgccgctac cggccggctc gccgctacca ttgcccacga gatcaacaat
     4501 ccgctcgaat ccgtcctgaa ccttatctac ctggcgcgca cctcgcgcgc agagatcgat
     4561 cgaatccgcg aatatctggg aactgccgaa aaggaactca cccgcgtcgc tcacatcgcg
     4621 cgccataccc tcggcttcta ccgcgaaacc tcgctcccca ccgacatcga tctgctggcg
     4681 ctcatcgacg aagtcctcac cgtctacgaa agccgcctgc gcgcctcctc catcgaggtc
     4741 cgccgcgact ttaccatcgt cccgacgctg cggggactcc gcggtgagct ccatcaggtc
     4801 ttctccaatc tcatttccaa ctccatcgat gccatgcgtg agggcgggac cctgaccctc
     4861 tccatccgcc aacccggcga agtccgggat cgggttgtcg tcgccgttca ggacaccggc
     4921 atgggcattc cccaggagaa tatcccgcgc ctcttcgagc ccttcttcac caccaagccc
     4981 agcgctggta ccgggctcgg cctctgggtc gtccgccagt tcgtcacatc ctggggcgga
     5041 aatgtggaag tccaaagcga gaccgatagc gcccggcacg gcactacgtt tactctgttg
     5101 ctgcccctgg tggccgtatc ggaaaaacgc gccgtaccgg aacaggtttc ggcgcgtctc
     5161 atgtaaatgt ccctggaaga gggccccttg ctcgatcgca ttctctggct tcagtccgct
     5221 gccccacccc ctccgccacc gcatgtttcg agtgacggtg aacccgcacg gtcgcgcacc
     5281 actcttcgcg ttctcgtggt cgacgacgag cgcctcatcg cggacacggt cgcggtcatc
     5341 ctctccaaca gtggattctc ggccgtcgcg gcctacagcg gcgagcaggc actggaagcg
     5401 gctcgctcgc agcaacccga catcgttctc accgatgtgc tgatgcccaa aatgaccgga
     5461 gtcgaactgg gcattcagat tcggagcgaa ttcccgcagg tgcgcgtgct gctcttctcc
     5521 ggccaggcgg ccaccagcga gctcatgcgc aaggcccacg accagggcca cgacttcgag
     5581 ctcttcccca agcccattca cccggaagaa ctcatcgcga agctgaagac gcgctggtag
     5641 ccgggttctc cggcaacaca gcacccggcc gttcagaaat cgtggagggt gccgtcgatg
     5701 cggatcttcg agcaccagaa gcaatagtcg actgccctgc ccggcacgat gtttgcaagg
     5761 tcgagtttcc gccagcgatg caggccgcag cggcagaaca aacccatcac ggcattcgat
     5821 tcgtgttcgc gatgcggcca cagtctggca agcgagcggg ctagctccgc ttcggaaggc
     5881 tgctcatttg cgtttgccat gatcggttag ctgtccagtt ttttcgttac cagctcattc
     5941 agcagcgccg ggttcgcctg cccctttgac gccttcatga cctgtccgac gaaaaaccca
     6001 gccacggtct tcttgccctt gcggtattgc tccacctgcg caggattggc cgcgatcacc
     6061 tcatcgatca tcttctcgat caccgaggaa tccgtaatct gctgcggctt ctcgcgctcg
     6121 tagaccgcgg gaaaatcctc tttgcgctcg aagcattgat cgaaaagctg cttgagctgt
     6181 ttgctcgaga gtttgccgtc ctcgctcaga tccgccgcga gcgcgatgcc cttcatcgag
     6241 atcggcgact gctccagttc aagatccgcc gctttgagcc gcatggtcag ctcgctctgc
     6301 accagattcg caacccgccg cggattcttt gccgccctcg cagcctcttc gaactgatcg
     6361 gcgaacgcgc gcgttgctgt gagcgtctgc gcatccttct cggtgatccc atactcggcg
     6421 accattcgcg cccgccgcgc ctccggcagc tcgggcatgt gcgaggcaat ctctgccttc
     6481 cacgcctcgg tcacgatgag cggcggaaga tccggctccg ggaagtagcg gtagtcgtgc
     6541 gcctgctcct tcgaacgcat ggggtaggtc cgcccttcgc tcgcgttgta gaggcgcgtc
     6601 tcctggacca cgcggccacc ggactcaacg atctcaatct gccgctcgat ctcgtactcg
     6661 agcgcggccc gaataaaacg aaaggaattg acgttcttga cttcggcctt ggtcccgaat
     6721 ttctcctgcc cgcgcggccg cacgctgaca ttcgcatcgc accgcagcga tccctcttcc
     6781 atattcccgt cgctcacacc cgtgtagacg atgatctcct tcagccgcgt caggtactcg
     6841 aaggcttcgt caggcgtgcg catgtccggc tcgctcacga tctcgatcag cggggtcccg
     6901 cagcggttca ggtcgacgta cgtcctcgtg gccgagtcgg gaaaaccgtc gtgcaggctc
     6961 ttccccgcgt cctcttccat gtgcagccgc gtgatgccga tgcgcttctt gctgtcaccg
     7021 ctgggcacct cgatccatcc gtgctcgccc agcggcttat cgaactgcga gatctggtag
     7081 cccttgggca ggtccggata gaagtagttt ttgcgggcga aaatggagcg ctcgcgcacc
     7141 tggcagttca gcgccagcga tgccagcgtg gcgaactcaa ccgcgcgctg gttcagcact
     7201 ggcagcgctc ccggcaagcc gaggcaaacc gggcagacat tcgtgttcgg cggcgcgccg
     7261 aatttgttgg cgcagccgca gaaggtcttc gtctcggtca gcagctgcac gtgcacctcg
     7321 agaccgatca cggcttcata cctggcgagg acttccggcg aaagtgtcac agcggtggcc
     7381 atgggaagtt ccagtgtaaa acgtgccact gggaaccgtg gaaacgtggc cgctccgcgg
     7441 cggccttcct cgacgggatg ggcgtttctg cagttccctt accttgtcat tccgaccgca
     7501 ccctgagcat agcgaagggg aagcggagga acctgcttct ttgtcgcgcg caacaataga
     7561 agaaaattcg gaaactcagc tctcagtccc cagccccagc aggaactacc acttcatcgc
     7621 gacgaacgag cacgctggcg accttccgcg tcaacgccac caccgcaccg gcatagctga
     7681 gactcagccg cgggatccgc ttcaaatcag tcagcaaagt atgcgactcc gcccccacca
     7741 gcagcgcacc gttgcggatg atgtgccagt gg
//
LOCUS       DASZYN010000717         4651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_104221, whole genome shotgun sequence.
ACCESSION   DASZYN010000717 DASZYN010000000
VERSION     DASZYN010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104221"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1387
                     /locus_tag="VHE33_18480"
     CDS             <1..1387
                     /locus_tag="VHE33_18480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99492.1"
                     /translation="LSYEVQQLADPSFFSARNTGLVRAFRELTPQGVLRLGGNTSEFA
                     WWKPTPDSPEPEHPKTREVAGEPKASYYAVTGEAVENLAGFLKATGWSCLYGIGMGTN
                     TPARAAEEAAFVSKTLGDRLQYFQIGNEVDLFDRHLRDPKTWSAKTYLAEWLTLARAV
                     EARVPGAKFGMPDVASNVKWLTEIAAEWRGINNPPHVTTLSHHYYFGGPATNPDVNIP
                     NLLKAATMAKVQRTADLATAAADKIGARVRMTEGNTCYRGGKPGVSDVFASALWSADY
                     SLLLASNNYSGLNLHGGTGKSVANSVGGSLPGDALLQQQGETPAQIATHPHPFYTPVA
                     TFGQDYVLEPVARGLQFAGSLSGARLIKADLTSKLQATGVNATAYAAELKGNRRSVVI
                     LNKDLEKDLDVVLSFAKGGGLEVETAKLEATKVDSRDAHIVQSPKSRLGDDGRYRVSV
                     PRVSGIRVTVA"
     gene            complement(1405..1620)
                     /locus_tag="VHE33_18485"
     CDS             complement(1405..1620)
                     /locus_tag="VHE33_18485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99493.1"
                     /translation="MRSNAMLHPATHATPNPGHRRLPSRSKIICIHCYRKLGMAASAQ
                     KSDQLLHSHDCAESRLAKEPGAPPPFN"
     gene            complement(1870..2901)
                     /locus_tag="VHE33_18490"
     CDS             complement(1870..2901)
                     /locus_tag="VHE33_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99494.1"
                     /translation="MKAIHKLFLSGSIFALCATPAFPGISISKPGNGAAVSSPFTLTA
                     RATSCSAQPVLVMGYSFDSNPSTRYSFGPSLNASVSAPAGGHTLHVKAWGWRGASCVA
                     DVSVNVQQNQNVVLAALTVPVWAVSVSNIQSLMDWSGTNDAASSGTSTGATSIVSSPS
                     MFGNAREFDVNFTDSGDERFYDNFGDDATSTNFIYDGYVYLDSSSSQIGNLEFDLDQV
                     MSNGQTVIFGVQCDGWNNTWDYTANLGTPTNWVVQWVHTPQPCNPRTWSTNTWHHIQI
                     VYSRDDNGMVTYKLIALDGNAIPINATALSAFALGWGPALLTNFQIDGYGSSGSATVW
                     LDGLNIYRW"
     gene            3367..4539
                     /locus_tag="VHE33_18495"
     CDS             3367..4539
                     /locus_tag="VHE33_18495"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HVT99495.1"
                     /translation="MILGILKESAPGETRVSLLPESLKSLIAQGLSICVESGAGEAAG
                     AADEAYAQAGAAIASGRNWILANADILPVVSAPPPVDQAALKPGVVVVGFLRPLDNPG
                     ALADAIDRPATLFSMELIPRITRAQSMDALSSMATVAGYKAMITAADRLPRLFPMLIT
                     AAGTIPPARVLVIGAGVAGLQAIATARRLGAVVEAWDVRAAAGEQVRSLGAKFLDLDL
                     GGINTEGGGGYAAELSEDALDRGRDLITQVAARADVIVTTAQVPGRPAPKLIRRTAIE
                     GMRPGSVIVDLAAPAGGNCEVTRPGLTQTIPAKNGGAVTLLAPLNLPAEVPVDASHLY
                     ARNVLNFLALIVRNGALNIDFGDEILAGACVAHQGNPVNPRVAQLLAVHAPELAAH"
BASE COUNT          916 a         1430 c         1428 g          877 t
ORIGIN      
        1 cctttcctac gaagtgcagc agttggccga tcccagtttc ttttcggcca ggaataccgg
       61 tttggttcgc gctttccggg aactgacccc tcagggagtt ctgcgcctgg gaggcaacac
      121 gagtgagttc gcctggtgga agcccacccc tgattctccc gagccggagc acccgaagac
      181 gcgggaagtc gccggcgagc cgaaagcgtc gtactacgcc gttacaggcg aggcggttga
      241 gaatctggcg ggtttcctga aagcgaccgg ttggagctgt ctttacggca tcggcatggg
      301 gaccaatact ccggctcgcg ccgcagaaga ggcggcgttc gtcagcaaaa ccctgggcga
      361 ccggctgcaa tattttcaga tcggcaatga ggtggacctg ttcgatcggc acctccgcga
      421 tcccaaaacc tggtcggcga aaacctacct ggcagaatgg ctcacgctgg cgcgggcggt
      481 ggaggctcga gttccgggcg caaagttcgg catgcccgat gtcgcatcga atgtgaagtg
      541 gctgacggag attgccgcag agtggcgggg catcaataac ccgccgcacg tgacgactct
      601 gagtcaccac tactacttcg gcggcccggc caccaaccct gacgtgaaca ttcccaacct
      661 gctgaaggcg gcgacgatgg ccaaggtgca gcgaactgcc gatctggcta ccgctgctgc
      721 ggacaagatc ggtgcgcgtg tccgcatgac cgaaggaaac acgtgttatc gcgggggaaa
      781 gcccggcgta tcggatgtct tcgcctccgc gctgtggtcg gccgactatt cgctgctcct
      841 tgccagcaac aactactcgg ggttgaacct tcacggtgga acagggaagt ccgttgcgaa
      901 ttctgtcggc ggctcgctgc ccggagatgc gcttctgcag cagcaagggg agacgccagc
      961 acagattgcg acccatcctc atccgttcta cacaccggtc gcgaccttcg gccaggacta
     1021 tgtcctggag ccagtggccc gtggactgca gttcgccggt tcgttgagcg gcgcaagact
     1081 gatcaaggcg gacctgacct cgaagctgca ggcaactgga gtcaatgcaa cagcctacgc
     1141 ggcagaatta aaggggaatc gcaggtctgt cgtgattctg aacaaggatc tcgagaagga
     1201 cctcgacgtc gttctgagct tcgcgaaagg cggtggcctc gaagtggaga cagcgaagct
     1261 ggaagcgacc aaagtagaca gccgcgacgc tcacatcgtg cagtcgccaa aatctcgttt
     1321 aggggatgac ggacggtatc gggtctccgt tcctcgtgtg tccggcattc gcgtgaccgt
     1381 ggcgtaaatc gccagcaaga acgcctaatt gaaaggaggc ggcgcgcccg gttctttggc
     1441 aagacgggac tcggcgcagt cgtggctgtg cagcagctga tcgctcttct gcgcgctggc
     1501 cgccatcccg agtttgcgat agcagtgaat acaaatgatt ttgctgcgag agggtagacg
     1561 acgatgtccc gggttggggg tggcatgcgt ggcgggatga agcatcgcat tgctcctcat
     1621 gaagattcct ccgtgaccgg gggcctggaa ccccgagggg gaagacacgc agaaactccc
     1681 aggaaagtgc tgcggtgcag atcgagaaag aacaatcgga ggcgtgatct caggccgaaa
     1741 gcaacggttc accggtcttt tcgaacgcca gcctacagat ataagacttc gcgcggagaa
     1801 ggctgtctgt gatcgccatc acatttttgt ccggaaccct ggcagaagga agttgccgga
     1861 tcggctgggc taccagcgat aaatattcag accatcgagc caaacggtgg cggatccgct
     1921 cgaaccgtaa ccatcgatct ggaaatttgt aagcagagcc ggaccccatc cgagagcgaa
     1981 tgcagagaga gcggtagcgt tgatggggat cgcgtttcca tcaagagcga tcagcttgta
     2041 ggtgaccatg ccattgtcat cgcgggagta cacgatctgg atgtggtgcc atgtattggt
     2101 gctccacgta cgcggattac agggctgagg cgtgtggacc cactgcacaa cccagttcgt
     2161 gggcgttccg agattggcgg tgtagtccca ggtgttgttc cagccatcgc actgaacacc
     2221 aaagatgacc gtctggccat tgctcatgac ctgatcgaga tcgaattcca ggttgccgat
     2281 ttgggaagag ctgctgtcga ggtagacgta gccgtcgtaa atgaaattcg tggatgtggc
     2341 atcgtcgccg aagttgtcat agaagcgctc atcgccggag tcggtgaagt tcacgtcgaa
     2401 ctcccgcgca ttgccgaaca ttgagggcga gctcacgatg ctggtagcgc cggtagacgt
     2461 gccggaggat gccgcgtcgt ttgtgccgga ccagtccatg agggattgaa tgttgctcac
     2521 actgacggcc cacaccggca cggtgagggc tgcgagcacg acattttgat tctgctggac
     2581 gttgacggac acatcggcga cgcacgatgc tcccctccat ccccatgctt tgacgtgcag
     2641 ggtgtgccca ccggcaggag cgctcacaga ggcgttcaga gatgggccaa aggagtagcg
     2701 ggtggaggga ttgctgtcga acgaatagcc catgacgagg acaggctgcg cggaacagga
     2761 agtggcccgg gccgtgagtg tgaagggcga agacacagca gcgccattgc cgggtttgct
     2821 gatgctgatg ccggggaatg ctggggtcgc gcatagagcg aaaatcgagc cgctcaaaaa
     2881 aagcttgtga attgccttca tagggccccc tggatgaaaa gtggccgtac atacggacta
     2941 cacccgaggc tgcggagcaa cgctcgcacg ggcaaaacgt ccatggtccc cgggtgggga
     3001 gcccggctgg gatcagggac gcccgagtct gccggcgcgg atgggtcgaa cacaaccgcg
     3061 cgggtcgaga tatcagtcgg ttattaagcg gattaataca cagatatcaa accaaaggga
     3121 agctctttct tcagacttcg ccgcgaaagt tgaggatttc gccattctcg agcgtcccga
     3181 agggctgatc gccttcagcc agtgatcgct gaggagccgg gataccgacc cctggggctc
     3241 gtgaccgtca tcacactgtc ccgccgccga tccggccaat aatgtctctt gcgccgttgc
     3301 gcggtcttgg tttccaccga gtggtgccgc ccggcttgcc ccgaaatcga catcgcggag
     3361 gcgcccttga ttctcggcat actgaaagag tccgcacccg gcgaaacgcg cgtctcactt
     3421 cttccggaaa gtctcaaatc cctcatcgcc cagggcctct ccatctgcgt tgaatccggc
     3481 gccggtgaag ccgcgggcgc cgccgacgag gcctatgccc aggccggtgc cgcaattgct
     3541 tccggccgaa actggattct cgccaacgcc gacatccttc ccgtcgtcag cgcgccgccg
     3601 ccggtcgatc aggccgcgct taagcctggc gtcgtcgtcg tgggcttcct tcgtccgctc
     3661 gataatcccg gcgcgctcgc cgacgccatc gatcgccccg ccacgctgtt cagcatggag
     3721 ctgatcccgc gcatcacccg tgcgcaatcc atggacgcgc tttcttccat ggccaccgtc
     3781 gccggctaca aagcgatgat caccgctgcc gaccggctgc cgcgcctctt cccgatgctc
     3841 attactgcgg ccggcaccat acctcccgcg cgcgtcctcg tcatcggcgc gggagtggca
     3901 gggcttcagg ccattgccac cgcacgccgc ctcggcgccg ttgttgaagc ctgggatgtc
     3961 cgcgccgccg ccggggagca ggtccgttct ctcggcgcga agttcctcga cctcgacctc
     4021 ggcggtatca ataccgaagg cggaggcggt tacgccgccg aactcagcga agacgccctg
     4081 gaccgcggcc gcgacctcat cactcaggtt gccgcccgcg ccgacgtcat cgttaccacg
     4141 gcacaggtcc caggccgccc tgctcctaag ctgatccgtc gcacggccat tgaaggcatg
     4201 cgccccggct ccgttatcgt cgacctcgcc gccccggcag gcggcaactg cgaagtcacc
     4261 cggccgggcc tcacgcaaac catcccggcg aaaaacggcg gtgcagtcac tctcctcgcg
     4321 cccctcaacc tgcctgcaga agtccccgtt gacgcgagtc acctctatgc ccgcaacgtc
     4381 ctcaacttcc tcgccctgat cgtcagaaat ggcgcactca atatcgactt cggggacgag
     4441 atcctcgccg gcgcgtgcgt cgctcaccag ggaaatcccg tgaatccccg cgtcgctcaa
     4501 ctcctcgctg ttcatgcgcc ggagctggcc gcccactgat cggaggaatc atgaaccccg
     4561 ccgtcctcat cgcttcggtt tatgtcttcg cgctggcagc cttccttggc tatcaggtca
     4621 tcagccgcgt tccgcctctc ctgcacacgc c
//
LOCUS       DASZYN010000718         2198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_949361, whole genome shotgun sequence.
ACCESSION   DASZYN010000718 DASZYN010000000
VERSION     DASZYN010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949361"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2198
                     /locus_tag="VHE33_18500"
     CDS             <1..>2198
                     /locus_tag="VHE33_18500"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HVT99496.1"
                     /translation="QESRSIAAISHPNVCTLHDVGPNYLVMELVEGETVAARLRRGKL
                     TLEQTLRYGAQIAAALAVAHARGIVHRDLKPANIMLTKAGVKVLDFGIARSPGDPTLT
                     QPGHVIGTPAYMAPERAEGKEADERADIYALGLILVEMATGQSSKFSAHLPPALERVI
                     RRCLEPDPDERWQSARDLQWELESIAQAPPAPAARPRVPLWLQAVAVIALAALAVFAF
                     LRFRQRPVPPALARLSVLLPEKSLVRSLAVSPNGRFVAVVLVKDGKQQIWIRAFDAVD
                     MTPLEGTDDAVDPFWSPDSRFLAFFADSKLKKVERTGGPVQTLCDSLGAVGGTWNRNG
                     DILIGALDQVQRVSESGGAPTKIPGHANITELFPVFLPDGRHYLAMRLGGPEPGLWLD
                     SMDGPQARRILPDLARAQVVTGASGGSNGAILFTRIGTLMALPFDENKLEAAGEPYPV
                     AQQIAVGTNESWLASSSWNGVVAYVAGEFGNRRYVWRDREGADLGTFGNAGGVVMISP
                     DGKRLVGDPRGNIEIQDLASGANRRLTVGAEQGNNPVWSPDGRSVVYSVAGGLHRIAT
                     DGGTPPEQLLRSRILVFAKSWSPDGRYLIYAQVHPGIGADLFALPIGQPGAQPMALWP
                     TPAASGQGEFSPDGRWVAVTSNESGQSEIYVIPFPPASDGVRWLISRGGGVQPRWRRD
                     GKELFYISPDWKMMAVDVDTQTSFRVGTPHALFNTEMLDTGIRNGPMSWD"
BASE COUNT          387 a          732 c          670 g          409 t
ORIGIN      
        1 caggagtccc gctccatcgc tgccatcagc cacccgaacg tgtgcaccct ccatgacgtc
       61 ggccccaact acctggtcat ggaactggtg gaaggcgaaa cggtggccgc gcggctcagg
      121 cgcggcaagc tgacgctgga acaaacactc cgttacggag cccagatcgc ggcggcactc
      181 gccgtagccc acgcgcgagg catcgtccac cgcgacctca agccggcaaa catcatgctg
      241 acaaaggccg gcgtcaaagt tctggacttc ggcatcgccc gttcgccagg cgatcccacc
      301 ctcacccagc ccggccacgt catcggcacg cccgcctaca tggcgcccga gcgagccgaa
      361 ggaaaagaag ccgacgaacg cgccgacatc tacgcgctcg gcctcatcct ggtcgagatg
      421 gcgacaggac agtcctccaa attttccgcc catcttcccc ctgccctcga acgcgtcatt
      481 cgcagatgcc tcgaacccga tccggacgaa cgctggcagt ccgcccgcga cctccagtgg
      541 gagctggagt cgatcgccca ggcgcccccc gctccagctg cgcgccccag ggttccactc
      601 tggctgcagg cagtcgcagt catcgccctc gccgcgctcg ccgtctttgc attcctgcga
      661 ttccgccagc gccctgtccc acctgcactg gcgcgcctca gcgtcctcct tccggagaaa
      721 tcgctggtcc gttcgcttgc cgtttctccg aacgggcgtt ttgttgcagt ggttctggtc
      781 aaggatggca aacagcaaat ctggattcgc gccttcgacg ctgtggacat gacgccgctg
      841 gaaggaaccg acgatgccgt cgacccgttc tggtcgcccg acagccgctt cctcgccttc
      901 tttgccgatt ccaagctcaa gaaggttgag cgcaccggag gcccggtaca aacgctgtgc
      961 gactctctcg gagccgtggg tggaacctgg aatcgcaacg gcgacatcct gatcggtgcc
     1021 ctcgatcagg tgcagcgcgt ctctgaatcc ggaggagcgc cgacgaaaat ccccggacat
     1081 gcgaacatca cggaactttt tccggtcttc ctgcccgatg gtcgccacta ccttgccatg
     1141 cgtttgggcg gaccggagcc agggctatgg ctggattcga tggatggacc ccaggcgcgg
     1201 cgaatcctgc ccgatctggc gcgggcgcag gtggtgacgg gagcttccgg cggcagcaac
     1261 ggagccatcc tgttcacgcg tatcggcacg ctcatggcgc ttcctttcga tgagaacaag
     1321 ctggaggccg ccggcgagcc ttatccggtt gcccagcaaa ttgctgtcgg caccaacgaa
     1381 agctggctcg catcctcctc atggaatgga gtggtggcgt acgtcgccgg cgagttcgga
     1441 aaccgccgct atgtctggcg ggatcgcgag ggcgcggatc tgggcacctt cggcaatgcc
     1501 ggcggtgtcg tcatgatctc cccggatggg aagcgcctgg tcggcgatcc ccgcggcaac
     1561 attgagatcc aggatttggc atcaggcgca aacagacgcc tgaccgtggg agcagagcaa
     1621 ggcaataatc cggtgtggtc gcccgatggc cgctcggttg tttactcggt tgccggcggc
     1681 ctgcatcgca tcgccaccga cggcggtact ccaccggagc agttgcttcg ctcaaggatt
     1741 cttgtttttg ccaagagctg gtcgcccgac ggacgctacc tgatttatgc ccaggttcat
     1801 ccgggaatcg gggcggatct gttcgcgttg cccatcggtc agccgggcgc gcagccgatg
     1861 gctttgtggc caacgccggc tgcgtccggc cagggagagt tttcgcccga cggacgctgg
     1921 gtcgcggtga cctcgaacga atccggccaa agcgagatct acgtcattcc ttttcctccc
     1981 gcgtccgatg gcgtccgatg gctcatctcg cgcggtggcg gcgtacaacc gcgctggcgt
     2041 cgcgatggca aagagttgtt ctacatctcc cccgactgga agatgatggc ggtggatgtg
     2101 gacacgcaaa cctcgttccg cgtcggcacg cctcacgctt tgtttaatac agagatgctg
     2161 gataccggca tccgcaacgg gcccatgagc tgggatat
//
LOCUS       DASZYN010000719         2888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_366574, whole genome shotgun sequence.
ACCESSION   DASZYN010000719 DASZYN010000000
VERSION     DASZYN010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366574"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..447)
                     /locus_tag="VHE33_18505"
     CDS             complement(55..447)
                     /locus_tag="VHE33_18505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99497.1"
                     /translation="MAIGITSCQPGNHGPYLSIARLKQIADNVPIPPGVTRISEQSGT
                     SFNGTTGVKSNEDTREFQTAIPCSQLEQSWLNLLRERHRTWTVLSPSPPEHHEIRIQD
                     SEATINIDFGVTYPNSCRQFGMVVDDLK"
     gene            complement(615..>2888)
                     /locus_tag="VHE33_18510"
     CDS             complement(615..>2888)
                     /locus_tag="VHE33_18510"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxKF domain-containing protein"
                     /protein_id="HVT99498.1"
                     /translation="TTPRTSEKSNTTSLDATILVGLGQVGAGSAYAAAGITFEGFPGT
                     SAPPSTLGPYTMAGFPEDPQPIANADLHGPGGRVSGVDGPTGVLAFDPPLYHELANHP
                     GWGTWSHGYEGDVYFTGPSGVKATVSLPNGTRAFYFYAEPNEFDTFKVTATTQDGSTS
                     GPIDVNGLDGARYFGFYTDGTVPLASVTITTSLDANGFAIGEFGISAGPISSISLLGM
                     GDSYSSGESVPPYSDPDGCHRSTRAYAPLLAQANNWNLADFTACTGATSLDFTFRGQH
                     GEQAQLAQLQTDRANGLHPDGIMLTLGGDDIGFKSIIEYCIEERIAGLDPLNFTRFGG
                     SCSDDPNFAPVTQDTIENEYAPLVETFRRLVATAGPDVSIMAADYPQIFPPNADQQTC
                     IELRPYFTKDDQSWFRLLTAILDNKELQAARDAGINGVNVLDEFTGHAICDSDGAWLN
                     GVTNINGWDDPTGSFHPTANGQIGYARAFANYINSAISAGTPLTSAGLPADPSDPPAD
                     NTSNTLQLTLGSLTVTPPVNQPDCTDVVQGGEQVTVTGDGYAPGAKVSLDLIDSDKAG
                     DAQHVALGTLTADSNGAVNTTVRLPLGMAGIVPPETSGSLAYLQTLGTGATAANQLDN
                     ELVNVAPHGNACGVVSALPFQGFDPPVANYPAVNAAQPGSTVPVKFTMTGVPGTIGDA
                     LAPGYPQSAPVSCSNPAALTSGDATSSTSLASRGGQSDHYNYAWATDRSWTGCRELIV
                     QVADGGYHQAVFNFSKK"
BASE COUNT          516 a          827 c          962 g          583 t
ORIGIN      
        1 tcgtcctact tcaagaccag aagtcgcgcc tgctcagcgc tcaacggacg ctctctactt
       61 cagatcgtcc acgaccatcc cgaactgcct gcaactgttc ggatacgtaa caccgaagtc
      121 gatgttgatg gtcgcctcag agtcctggat ccgaatctca tgatgctctg ggggcgacgg
      181 actcagaacc gtccaggtgc ggtgccgctc tcgtagcaga ttgagccagg actgttcgag
      241 ctgactgcat ggaatggcag tttggaattc ccgagtgtcc tcgttcgact tcacgcctgt
      301 ggtgccgttg aaagaggtgc cgctctgttc gctgatccgt gtcactccgg gtggaatggg
      361 cacgttgtcg gcgatctgct tgagtcgcgc gatcgatagg tagggtccgt ggttgcccgg
      421 ctgacacgaa gtgattccga tcgccaacac aacgttcagt agcaggacgc cccgactacc
      481 ggcccgccgg gtggccatca caggtcgtga gcgttgagga ggaatggcac acctcgttca
      541 cttacagcag ccgagagcga gcggctcggg acttgccgga tcaggtcatt atcgccaact
      601 gcgttggctc ggcgctactt cttgctgaag ttgaaaaccg cctgatgata gccgccgtcc
      661 gcgacctgca cgatgagttc tcggcagccg gtccaggacc ggtcagttgc ccatgcgtag
      721 ttgtagtgat cggattgtcc gccccgactc gcgagggagg tcgagctggt tgcgtccccg
      781 cttgtgagtg cagccgggtt gctgcaagac accggggcgg actgggggta tcccggagcg
      841 agcgcatcac cgatcgtgcc gggaaccccc gtcatagtga acttgactgg aacggtcgag
      901 cccggctggg cagcgttgac ggcggggtag ttggctactg gtgggtcgaa gccctggaac
      961 ggcagtgcgc tgacgacgcc gcaggcgttg ccgtgcgggg cgacgttgac caactcgttg
     1021 tcgagttgat tcgcggcggt cgcgcccgtc cccaatgttt gcagataggc cagcgagccc
     1081 gaggtctcag gcgggacgat ccccgccatg ccgagcggaa gccgaacggt cgtgttgacc
     1141 gcgccattgc tgtctgcggt gagggtgcct agagcgacgt gttgagcgtc gcccgccttg
     1201 tccgagtcga tgaggtcgag tgacaccttg gcgccgggcg cgtagccgtc gccggtgacc
     1261 gtgacttgct cgccaccttg gacgacgtcg gtgcagtcgg gctggttgac cggcggggtc
     1321 accgtcaacg agccgagggt cagttgcagt gtgttacttg tgttgtcggc tggtgggtca
     1381 ctgggatctg cgggaagccc ggctgaagtg agcggggtgc cggcagaaat cgcactgttg
     1441 atgtagttgg cgaaggcccg cgcgtacccg atttgcccgt tggcagtggg gtggaaggac
     1501 ccggtggggt catcccatcc gttgatgttg gtgacgccgt ttagccacgc gccgtcgcta
     1561 tcgcagattg catgcccggt gaactcgtcg agcacgttga ccccgttgat gccagcatcg
     1621 cgcgccgctt gcagctcctt gttgtccaag atcgcagtga gcagacggaa ccaggattga
     1681 tcgtccttgg tgaagtacgg ccgcagctca atacaagtct gctgatcggc gttcggaggg
     1741 aagatctgcg gatagtcagc cgccatgatg gacacgtccg ggccagcggt cgcaacgaga
     1801 cgtcggaaag tctcgaccaa cggcgcgtac tcgttttcaa tggtgtcttg tgttactggc
     1861 gcgaagttcg ggtcgtcaga gcagctgccg ccgaatctcg tgaagttgag cgggtcgagt
     1921 ccagcgatac gctcctctat gcagtactcg atgatcgact taaagccgat gtcgtctccg
     1981 cccagggtga gcatgatccc atccgggtga agcccgttgg cacgatccgt ctgcaattgg
     2041 gccagctgag cttgctcgcc gtgctgcccc cggaaggtga agtcgaggct ggttgcgccc
     2101 gtacacgcgg tgaagtcggc gaggttccag ttgttggcct gggccaacaa cggcgcgtac
     2161 gcgcgggtcg atcggtggca accgtccggg tcggaatacg gaggcaccga ctcgcccgaa
     2221 gagtaggagt cgcccatacc gagcaggctg atcgaagaga tcggcccagc ggagatgccg
     2281 aactcaccga tcgcgaatcc gttggcgtct aagctggtcg tgatcgtgac ggaagctaac
     2341 ggcacggtgc catccgtgta aaacccgaag taccgtgcgc cgtcgagacc gttgacatcg
     2401 atcggtccgg atgtggaccc gtcctgtgtg gttgcggtga ccttgaacgt gtcgaactca
     2461 ttcggctcgg cgtagaagta gaacgcgcgg gtgccgttcg gcaatgacac ggttgctttg
     2521 acaccacttg gtccagtgaa atacacgtcg ccttcgtagc catgactcca agtaccccaa
     2581 ccagggtggt tagcgagctc gtggtaaagc ggcggatcga acgccaagac ccctgtaggg
     2641 ccatcaacgc cgctaacccg tccccccgga ccatgcagat cggcgttagc gattggctgc
     2701 gggtcttcag gaaacccggc catggtgtat gggccgagtg tggaaggcgg cgcacttgtc
     2761 ccagggaaac cctcaaaggt gatgccggcc gcggcgtagg cgctcccagc gccgacttgc
     2821 ccaagaccga cgaggatagt tgcgtccagc gaggtggtgt ttgatttctc gctcgtgcgt
     2881 ggcgtcgt
//
LOCUS       DASZYN010000720         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_237457, whole genome shotgun sequence.
ACCESSION   DASZYN010000720 DASZYN010000000
VERSION     DASZYN010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237457"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..802)
                     /locus_tag="VHE33_18515"
     CDS             complement(29..802)
                     /locus_tag="VHE33_18515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99499.1"
                     /translation="MKPRTNALLPRALSRFRFPVLALQWGDNLVLIILAVLWLQIPDS
                     HVWQFALSMISAVALVAGFCWIQIATFARIRPATERTNIWLRLFGFALVATLWFLLVL
                     WIGSGSDSIPSYAYLWNSKLSPGMRITLAPARIIASLNVVIDVIICAITALLLPVAIV
                     LSTDGLLRPTWRDARRPYRRILYWIAVFVFCTVVTQLTAALASWVPGRGVSGEVLSVA
                     LRLALAWTTDVLLWCLLLAFTGAWMEPAPAPLPFPKPAS"
     gene            complement(799..>2093)
                     /locus_tag="VHE33_18520"
     CDS             complement(799..>2093)
                     /locus_tag="VHE33_18520"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99500.1"
                     /translation="EDESRVFMTAYVVSGLGEASNYYKLDWRRQQMLNNGRDYLEKQL
                     AAHPRMIPDLRAYVVYALSESGSSNIGSQLNTLWSRRSDFSSEGLSFTGLAMLRAHDA
                     RAADLAKLLEGKVKEQGDLASWPSNYSPVLDLEYDDGAQSTAFAMRFLAHADAQSPLL
                     PKAAEWLMLNRNDGSWWDSTEQTAMVIFGLTDYLAGSQELNADFDASVRVNGAILGHR
                     HFTPADAIAGNTLDITVPTAALSAHNTIDIARTDGGRAYWTAQATYFSTEHKLYQQGS
                     LTLNLTRDYFRLTPGKDKNGNIVYNLDPLKGAAQIGDVLAVHIAIHGSPEKYLFIEDP
                     IPAGTEFIKNEDSYNIAGRPTDWTWWYTRREFHDDRAVLFATYFSGHQESFYLLKVVN
                     PGSFEISPARVEPMYQPGIEATSDELHLDIPDSSEVNP"
BASE COUNT          440 a          556 c          723 g          374 t
ORIGIN      
        1 cgctcgggga ccgtgccggc ggacgcgatc aggatgctgg tttcgggaag ggcagcggcg
       61 ccggagcagg ctccatccac gccccggtga aggcgagcag cagacaccaa aggagcacgt
      121 cagtcgtcca ggcgagagcg aggcgcaggg cgacgctgag gacctcgccg gagacgcctc
      181 gtcccggcac ccatgatgcc agtgctgcgg tgagttgggt gacgacggta cagaaaacaa
      241 acacagcgat ccaataaagg atgcggcgat acggccgccg tgcgtcgcgc cacgtggggc
      301 gaagaagacc gtcggtactg agaacgatcg ccacgggcaa cagcagggcg gtgatcgcgc
      361 agatgataac gtcgatcacg acattgaggg acgcgatgat acgcgcgggt gcgagagtga
      421 tgcgcatgcc gggtgagagt ttcgagttcc agaggtaggc atagctggga atgctgtcgc
      481 tgccggaccc aatccacagc accagaagaa accacagcgt ggcaacgaga gcgaagccga
      541 acaggcggag ccagatattc gtccgctccg ttgcggggcg gatgcgggca aaggttgcaa
      601 tctgaatcca gcagaagccg gcgacaagcg caacggcgga gatcatggaa agcgcgaatt
      661 gccacacgtg cgaatcgggg atctgaagcc agagcacggc caggatgatc agcaccagat
      721 tgtctcccca ttgcaaagcg agcaccggga agcggaatcg ggagagggcg cgaggaagaa
      781 gcgcattcgt acgtggtttc acgggttcac ctctgaagaa tcgggtatgt cgaggtgcaa
      841 ctcgtcactg gtggcctcaa tgccaggctg atacatcggc tccacgcgcg caggactgat
      901 ttcgaagctg ccggggttca cgactttcag aagatagaaa gattcctgat ggccggagaa
      961 ataggtggcg aagagcacgg cgcggtcgtc gtggaattcg cgccgcgtat accaccatgt
     1021 ccagtcggtg gggcggccgg caatattgta gctatcctcg ttcttgatga actcggtgcc
     1081 ggccggaatg ggatcctcga tgaagagata cttctccggt gagccgtgaa tggcgatgtg
     1141 gacggcaagg acgtcgccaa tctgtgcagc gcccttgagg ggatcgaggt tgtagacaat
     1201 gttgccgttc ttgtctttgc cgggcgtgag gcggaaataa tcacgcgtga ggttgagtgt
     1261 gagcgagccc tgctggtaga gcttgtgctc ggttgagaaa tacgttgcct gagcggtcca
     1321 gtacgcacga ccaccatcgg tgcgggcgat gtcgattgtg ttgtgcgcac tgagggcggc
     1381 ggtgggcacg gtgatatcga gtgtgttgcc ggcgatggcg tcggctgggg tgaagtggcg
     1441 atggccaagg atcgcgccgt tgacgcgcac actggcgtcg aagtcggcgt tgagttcctg
     1501 ggagcccgcg agatagtcgg tgagaccgaa gatgaccatc gcggtttgct cggtcgaatc
     1561 ccaccaggag ccgtcattgc gattgagcat gagccactcg gcagctttcg gcagcagcgg
     1621 gctttgcgcg tccgcatgcg cgaggaagcg catcgcgaaa gcagtactct gcgcgccgtc
     1681 gtcgtattcg agatcgagga cagggctgta attcgatggc caggaagcga gatcgccctg
     1741 ttctttcact ttgccctcaa gcagcttcgc tagatccgct gcacgcgcgt cgtgcgcacg
     1801 gagcatcgca aggccggtga acgacaggcc ctccgacgag aaatcgctcc gccgcgacca
     1861 caacgtgttg agctggctgc cgatgttcga tgagccggac tcggacagcg cataaacgac
     1921 gtaagcgcgc agatcgggga tcatccgcgg atgtgcggcg agctgcttct cgagatagtc
     1981 gcggccgttg ttcagcattt gctggcggcg ccagtcgagc ttgtagtagt tgctggcttc
     2041 gcccaggcca ctgacgacgt aggcagtcat gaagacgcgg ctctcgtctt ctt
//
LOCUS       DASZYN010000721         3527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_809778, whole genome shotgun sequence.
ACCESSION   DASZYN010000721 DASZYN010000000
VERSION     DASZYN010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809778"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VHE33_18525"
     CDS             <1..228
                     /locus_tag="VHE33_18525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT99501.1"
                     /translation="QGPGVELGEQPFIFDKFFRGSKQRGRAGGTGMGLAIVKAIVEAH
                     GGGLELVSEPGKGSRFAFWLPLEKQPTGSSQ"
     gene            complement(301..789)
                     /locus_tag="VHE33_18530"
     CDS             complement(301..789)
                     /locus_tag="VHE33_18530"
                     /inference="COORDINATES: protein motif:HMM:NF002696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-degrading domain-containing protein"
                     /protein_id="HVT99502.1"
                     /translation="MAIVDDVGRIALQERELVFSEFNEETAWRIGNRLRTLATERSLS
                     VVIDVRRLAQPLFYAALPGTTSDNPEWVRRKSNVVFRFHRSSYAVGLDLEQKHSSIEA
                     RGLPVADYAAHGGSFPIRVQNAGLVAAVTVSGLPQRADHELVVEALCAELNKDYASLR
                     LS"
     gene            complement(792..2039)
                     /gene="ispG"
                     /locus_tag="VHE33_18535"
                     /gene_synonym="gcpE"
     CDS             complement(792..2039)
                     /gene="ispG"
                     /locus_tag="VHE33_18535"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HVT99503.1"
                     /translation="MPEIQRRKTVTVRIGSVKVGSEAPVVVQSMTNTDTADPASTIQQ
                     VAELVEAGSELVRVTVNNDEAAQAVPHIVEGLAKRGIHVPIIGDFHYNGHLLLKKYPA
                     CAQALAKYRINPGNVSIGRKDDDNFRTMIECAVENDKPVRIGVNWGSLDQALLTRLMD
                     ENSKSANPAPARDVMLEAMCRSALDSAAAAERYGLGHDKIIISGKVSGVRDLIDVYQM
                     LAARCDYPLHLGLTEAGMGMKGIVASTAGLAPLLLQGIGDTIRVSLTPKPGSSRTEEV
                     LTAQQVLQSLGIRSFAPQVTACPGCGRTTSTFFQELAQQIQDYLRTSMPIWRQQYPGV
                     EELKLAVMGCVVNGPGESKHANIGISLPGTFEEPKAPVYVDGRLMTTLRGDTIVADFK
                     KILDDYVKNRYGSREHELEETAV"
     gene            complement(2130..3251)
                     /locus_tag="VHE33_18540"
     CDS             complement(2130..3251)
                     /locus_tag="VHE33_18540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT99504.1"
                     /translation="MLPRVPDLLAPQYDSHAGGHLDPNRLPQVTVIVPARDEGAAIEA
                     TLRSLLAQTIPLEILAVDDRSTDATGAIMDRIAAEAQTLGKYLRVIHIEELPPGWLGK
                     PHAMALAGRQTATPWLLFTDGDILFRQDTLERALRYAEDSADHLVLLPTLLLKTAGER
                     MMAAIFQSLTLLAWRPWKVADPKARRESIGMGAFNLVRSEAYRAVGGFEAAPLEVLED
                     LRLGYAIKRSGYRQRFVLGRGLIRLHWAPGAFGMVNNLTKNIYATFRFRPILFLGAWL
                     GLLLLCGTPLAGLLAHFWLIRITSVLSLLMIALLYQQASRFYTPVNPVYILTFPVALG
                     LVLYAMLRSMVVTLIRRGIVWRGTFYPLRELRRNAGPLR"
     gene            complement(3340..>3527)
                     /locus_tag="VHE33_18545"
     CDS             complement(3340..>3527)
                     /locus_tag="VHE33_18545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99505.1"
                     /translation="IHALGDSFSPGLIGHISDATNLRIGLGATLIALVLSGAILFGGS
                     RFAPRLPDLEASSKGLH"
BASE COUNT          721 a          966 c         1220 g          620 t
ORIGIN      
        1 caggggccag gagttgagct cggcgaacaa cctttcatct ttgacaagtt ttttcgcggc
       61 agcaaacagc gtggccgggc gggcgggacc gggatgggac tggcgatcgt gaaggcgatc
      121 gtggaggcgc acggtggcgg ccttgagtta gtcagcgagc cgggaaaggg atcgcgtttt
      181 gcattctggc tgccgctgga aaaacagcca acaggcagta gccaataggt agtagctgat
      241 gaagggtggc caatggccag ggcgcgctcg acagttcgtg gtgctgtggg gaaagccgga
      301 tcaggagagg cggagcgagg cgtaatcttt gttgagttcg gcgcagaggg cttcaaccac
      361 gagttcatga tcggcgcgct ggggcaggcc tgaaaccgtc acggccgcga ccagacccgc
      421 attctgcaca cgaatgggaa agctgccacc atgggcagca tagtcggcga caggaaggcc
      481 gcgggcctca atcgaagaat gtttctgctc aaggtcaagg ccaacggcgt acgaactgcg
      541 gtgaaagcgg aagacaacat tgctcttgcg gcggacccac tccgggttgt cggaggtggt
      601 gccgggcagg gcggcgtaga aaagcggttg ggcaaggcgg cgaacgtcga tgacgacgga
      661 aaggctgcgc tccgtggcga gagtacgaag ccgattgccg atgcgccagg cagtttcctc
      721 gttgaattcg gagaagacca gttcgcgctc ctgcagcgcg atacgcccca catcgtccac
      781 gatggccatg cttagaccgc tgtctcctcg agttcgtgct cgcgactacc atagcgattc
      841 ttcacatagt cgtcgaggat cttcttgaag tccgcaacaa tggtatcgcc gcgcaacgtc
      901 gtcatcaggc ggccgtcgac atagaccgga gccttgggct cctcgaatgt gcccggcaga
      961 gatatgccga tattggcgtg cttcgattcg ccaggcccgt taaccacgca ccccatgacc
     1021 gcgagcttca gctcttcgac gcccggatat tgctgccgcc agatcggcat cgaggtgcgg
     1081 agatagtcct ggatttgctg cgccagctcc tggaaaaacg tgctcgtcgt gcggccgcag
     1141 ccgggacagg ccgtgacctg cggcgcgaag ctgcgaatgc cgagcgactg gaggacctgc
     1201 tgcgcggtca ggacctcttc ggtgcggctc gagccgggct ttggcgtcag cgaaacgcgg
     1261 atggtgtcgc cgatgccctg aaggagcaga ggcgcgaggc cggccgtgga ggcgacgatg
     1321 cccttcatgc ccatcccggc ttcagtgagc ccaaggtgca gcggatagtc gcagcgggcg
     1381 gcaagcatct gataaacgtc gatcaggtcg cggaccccgg agactttgcc gctgatgatg
     1441 atcttgtcgt gcccgaggcc atagcgttcg gcggcagcgg cggagtcgag cgcagagcgg
     1501 cacatcgctt cgagcatcac gtcgcgcgca ggtgcgggat tggcggattt ggagttttcg
     1561 tccatcaggc gcgtgagcag cgcctggtcc agcgaacccc agttcacacc gatacgcacc
     1621 ggcttatcgt tctcgacggc gcactcgatc atggtgcgaa agttgtcatc gtccttacgg
     1681 ccaatggaaa cgttgcccgg gttgatgcgg tacttcgcaa gcgcctgcgc gcacgcggga
     1741 tacttcttta gcagcagatg gccgttgtag tgaaagtcgc cgataatcgg cacgtgaatg
     1801 ccgcgcttag caaggccttc cacgatgtgc gggacggcct gggcggcctc gtcattgttg
     1861 actgtgacgc gtacgagttc agagccggcc tcgacgagtt cggcaacctg ctggatggtc
     1921 gaggcggggt cggccgtatc ggtgttggtc atggattgca cgacgacggg ggcttcggag
     1981 ccgaccttga cgcttccgat gcggacagta accgtcttcc tgcgctgaat ttcgggcatg
     2041 gggacctcca ttacagggta cagggaacag ggcgcagggt acagaggact ggccgccctg
     2101 gcggacgcgc ttcgcgcagg atgctggggt caccgcagag ggccggcgtt gcggcggagt
     2161 tctctgagcg ggtagaaggt accgcgccag acgatgccgc gccggatgag ggtgacgacc
     2221 atagaccgca acatggcgta gagcaccagg ccgagtgcga ccggaaacgt aaggatatag
     2281 acaggattga cgggggtata aaagcgcgac gcctgttggt aaagcaacgc gatcatgagc
     2341 agtgagagca cgctcgtaat gcggatcagc cagaaatggg cgaggagccc ggccaggggc
     2401 gttccgcaaa ggaggagcag gcccagccat gcgcccagga aaagaattgg tcgaaaccga
     2461 aacgtagcgt agatgttctt ggttaggttg ttcaccatgc cgaaggcccc tggagcccag
     2521 tgcagacgga tcaggccgcg gccgagaacg aatcgttggc ggtagccgct gcgtttgatg
     2581 gcatagccaa ggcgaaggtc ttcgaggacc tcaaggggcg ccgcttcgaa tcccccgacc
     2641 gcgcggtagg cttcactgcg gaccaaattg aaggcaccca taccgatcga ctctcgtctg
     2701 gctttgggat cggcgacttt ccatggccgc catgccagca gggtaagtga ctggaaaatc
     2761 gccgccatca tacgttctcc cgcagttttg agaaggagcg tgggcagcag aacaagatga
     2821 tcagcggaat cctcggcgta tcgtaacgcc cgctccaaag tgtcctggcg aaagaggatg
     2881 tcgccatcgg tgaaaaggag ccacggcgtc gcggtctgac ggccggcgag agccatggcg
     2941 tgtggtttgc cgagccagcc gggcggcagc tcttcgatgt gaatcaccct gaggtacttg
     3001 cctagggttt gagcctcggc ggcaatgcgg tccatgatgg ccccggtcgc atcggtcgac
     3061 cggtcgtcga cagcgagaat ctcgagcggg atggtttgcg ccagcagaga gcgcagcgtc
     3121 gcctcgatgg cggcgccttc gtcgcgcgcc gggacgatga cggtgacctg cgggagacga
     3181 ttcgggtcga ggtgaccccc ggcgtgcgaa tcgtactgag gagcgagaag gtcaggaact
     3241 cgaggcagca tccgctgggc ggtggttgct cggtttgtcc agagaaccag gaccagttcg
     3301 ctgaaagatg ttgaaaggac caggggccag ttcatcggat caatggagcc ccttactgga
     3361 cgcttcgaga tccggcagac gcggcgcgaa gcgagaaccg ccaaagagga ttgcgcccga
     3421 gagtacgagc gcaatcaggg tggcaccgag accgatgcgc aggttggtgg cgtcggagat
     3481 gtggccgatg aggccaggag aaaaagaatc gcccagagcg tgaatga
//
LOCUS       DASZYN010000722         1947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_173317, whole genome shotgun sequence.
ACCESSION   DASZYN010000722 DASZYN010000000
VERSION     DASZYN010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173317"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VHE33_18550"
     CDS             <1..733
                     /locus_tag="VHE33_18550"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HVT99506.1"
                     /translation="TNELAAGSVGLTSGVMMASSTSIDVVIRGIGGHGAAPQVGRDPI
                     TLAALYITQIQTIVSREESPTDPTVVTVGDIHGGTKRNIIPDEVKLELTTRSFSDQSR
                     NTVIQGLKDMAAGITASAGLPPAKAATVTVLDDESTPVEYNDPAMTAQVKATLTKALG
                     ADHVVDLPRVMGSEDVGVFALPNHEIPLVYFRLGAMDPAKLAAAKAAGKELPGPHTSR
                     FEPLPEPTLRTGVTAMSSVAISLLQ"
     gene            complement(878..1150)
                     /locus_tag="VHE33_18555"
     CDS             complement(878..1150)
                     /locus_tag="VHE33_18555"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HVT99507.1"
                     /translation="MGHGIIAWIIIGVIAGWLTGKIMKGSGFGFLMDMVVGLVGALIG
                     GFISAHLGFGGVGEHGLIISIVIAVIGAVILTWIVRLITGNRKANL"
     gene            1334..1891
                     /locus_tag="VHE33_18560"
     CDS             1334..1891
                     /locus_tag="VHE33_18560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99508.1"
                     /translation="MFRKLLVFALFFVVLLPNRAHAQQTESWQAKVAHELPLLGHRNW
                     ILVVDSAYPLQSAPGIETLETRAPQTLVLRVVLGEIENSEHVRPDIYTDAELPYVSDE
                     DAPGISQFRANLADLLHAYPVQSQAHGKLIDRIDQDSQKFQVLVLKTNSVLPYSSVFI
                     NLKAKYWGDDAESRLRGKMGSGSQP"
BASE COUNT          412 a          612 c          567 g          356 t
ORIGIN      
        1 taccaatgag ctggccgcgg gcagcgtggg cctgaccagc ggagtcatga tggccagctc
       61 cacgtccatc gacgtggtga ttcgcggcat cggcggccac ggcgcggcgc cccaggtggg
      121 acgcgacccc atcacgctgg cggcgctcta catcacgcag attcagacca tcgtgagccg
      181 cgaagaaagt ccgacggatc cgacggtggt caccgtgggg gacattcatg gcggcaccaa
      241 acgcaacatc attcccgatg aggtgaagct ggagctgacg acgcgctcct tcagcgacca
      301 gagccgcaat accgtgatcc agggcctgaa ggatatggct gccgggatca cagcgtctgc
      361 cgggctgcct ccggcgaaag ccgcgaccgt cacggttctc gacgacgaat cgacgccggt
      421 ggaatacaac gaccccgcga tgacagcgca ggtgaaagct accctgacga aggcgctggg
      481 cgccgatcac gtggtcgatc tcccccgcgt catgggcagc gaggacgttg gcgttttcgc
      541 gctgccgaat cacgagattc cactcgtcta cttccgcctg ggcgcgatgg atccggcaaa
      601 attagcggca gcaaaagcag cgggcaaaga gctgcccggt ccacacacca gccgctttga
      661 gccgctccct gagccaacgc tgcgcacggg cgtcaccgcc atgtcgagcg tggcgatctc
      721 gcttctgcag tgaagaagcc atgaaactgg ctcaatcgtt cacaagactc cagaatttcc
      781 ggcgaaggcg gctggacctg cgtcggccag actcagtgaa gttctttgga acgcgcggcc
      841 tcgttcaagc gcgggcggtg gaccgtattc gctgggttca aagattggct tttcgattcc
      901 ccgtaatcaa ccggacaatc caggtcagga tcaccgcgcc gatcacagca attacgatgc
      961 tgatgatcag gccatgctcg ccgacgccgc cgaagccgag atgagccgaa atgaaaccgc
     1021 caatgagcgc tccgaccagc cccaccacca tgtccatcag gaagccgaac ccggagccct
     1081 tcatgatctt tcccgtcagc caacccgcaa tcactccgat aatgatccag gcgataatgc
     1141 cgtgtcccat gatgttcttc ccctttccga atcagtttcc cagtgaatag cgttgatagt
     1201 cctttgaagg aaaaaggtca acgggaaaaa agaggggtac cgctggcgcc agaaccaatt
     1261 cagccgcaca ggcgtctttg atttgtgcgt gacgcgagtt ccgctaccat agggacccca
     1321 gggagtcgat ttcatgttcc gaaagcttct tgtttttgcg ctgttttttg tcgtgctgct
     1381 tccgaatcgg gcgcatgccc agcagacgga gtcctggcag gctaaagtcg cccatgagct
     1441 gccgctgctc ggtcatcgca actggatcct ggtggtcgat tccgcctacc cgctgcaaag
     1501 cgcgccgggc attgagaccc tcgaaacgcg cgcgccgcag actctggtgt tgcgtgtggt
     1561 cctgggcgag attgaaaact ccgagcacgt gcggccggat atctataccg acgcagaact
     1621 accctatgtc tccgatgagg acgcacccgg catctcgcag ttccgcgcga acctggccga
     1681 cctcctgcac gcctatcccg tgcagtcgca ggcgcacggc aagctcatcg acagaatcga
     1741 tcaggacagt cagaaattcc aggtgctggt gctgaagacg aactcggtcc tgccctactc
     1801 gtcggtcttc atcaacctga aggcgaaata ctggggcgac gatgcggaat cgcggctgcg
     1861 cggcaagatg ggttccggaa gccagccgta agcgtctacc gcaaccccac cagcccccgc
     1921 accgattcga tcagcttttg cggatcg
//
LOCUS       DASZYN010000723         3058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1198512, whole genome shotgun sequence.
ACCESSION   DASZYN010000723 DASZYN010000000
VERSION     DASZYN010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198512"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VHE33_18565"
     CDS             complement(<1..530)
                     /locus_tag="VHE33_18565"
                     /inference="COORDINATES: protein motif:HMM:NF014930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99509.1"
                     /translation="MATAVTLSPEVLARYEAVIGLEVHVQLLTETKAFCGCANKFGAP
                     PNTNVCPVCLGLPGALPVLNRKAVEFATLASLALHCEVRERSVFARKNYFYPDLPKGY
                     QISQFDKPLAEHGWIEVPSGESKKRIGITRLHMEEDAGKSLHDGFPDSATRTYVDLNR
                     CGTPLIEIVSEPDMRTP"
     gene            complement(598..1179)
                     /locus_tag="VHE33_18570"
     CDS             complement(598..1179)
                     /locus_tag="VHE33_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99510.1"
                     /translation="MRDGGKFGTGAMPGSGWFYAITHWNWKVTLMAAALRGLACMLAL
                     RHVEHHARNHFGLVEATFVLFSSGFFSALQQQSLQIRSERFAWVICVVVIPLTSLSCD
                     AGLHFWLDGRDTFQLGPAALVFTVVSATFHWHIIRNGALLVGAESHTLLTDLKRIPRL
                     SLSYAGAVVALTRKVAGVLVRRDEVVVPAGAGD"
     gene            1613..3025
                     /locus_tag="VHE33_18575"
     CDS             1613..3025
                     /locus_tag="VHE33_18575"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HVT99511.1"
                     /translation="MSTHARIRSAIAALCLSAAPLLLALFLLAGCGMQSGNSVSNPGT
                     TSGSAFVIGTDAPLASVVSFNATLQAINAIDASNNSVALISGSPTVDFARFNGLQTLL
                     DMNSVPAGTYTQIQVVFSSATIGYLQTGSGAPTIQTMPATFTTNTVTKTLANPLVVST
                     AGPVGIHMDFRLDKSVQVSNGQITGDVNPTLDIRAVGPTDPDAYVDEFIAGVVNPTAS
                     SQSFTIQGPHGRTFTVNVNGQTEWDNGEKLSDLTSTSVVQISGSLDRADSTIDADEVA
                     ILTQNGFYAGGQITYVQPSSGPATAFQLYVRGTLPASGDGVSLGQIAQVNLSGSEKFF
                     IYWMHNPLTEFLFNSSTLLPGQHVTVGGALSGAADGNALSVKRVVLRNWGFNGTLVPN
                     SISNGTFQMNIAGFAGLLVPNQPVTVYTGPGTRFRLGLNAMADVSTATKIRVVGLLIK
                     DPTSGNSVLLAHYIDDLDKD"
BASE COUNT          595 a         1078 c          822 g          563 t
ORIGIN      
        1 ggcgtgcgca tgtccggctc gctcacgatc tcgatcagcg gggtcccgca gcggttcagg
       61 tcgacgtacg tcctcgtggc cgagtcggga aagccatcgt gcaggctctt ccccgcgtcc
      121 tcttccatgt gcagccgcgt aatgccgatg cgcttcttgc tttcaccgct gggcacctcg
      181 atccacccat gctccgccag cggcttgtcg aactgcgaaa tctggtagcc cttgggcagg
      241 tcgggataga agtagttctt ccgcgcaaac accgaccgct cccgcacctc gcagtgcagc
      301 gccagcgacg ccaaagtcgc aaactcgacg gccttgcgat tgagcaccgg cagcgcgccc
      361 ggcaagccga ggcacaccgg acagacattt gtgttcggag gcgcgccgaa tttgttggcg
      421 cagccgcaga aggccttcgt ctcggtcagc agctgcacgt gcacctcgag accgatcacg
      481 gcttcatacc tggcgaggac ttccggcgaa agtgtcacag cggtggccat gggaagttcc
      541 agtgtaaaac gggccgccgc gatcggctgg cggatgaccc ttccgcgcga cctgctctca
      601 gtctccagcc ccagcaggaa caaccacttc atcgcgacga acgagcacgc cggcgacctt
      661 ccgcgtcaac gccaccaccg caccggcata gctgagactc agccgcggga tccgcttcaa
      721 atcagtcagc aaagtatgcg attccgcccc caccagcagc gcaccgttgc ggatgatgtg
      781 ccagtggaac gtggccgaaa cgactgtaaa caccagagcg gcgggaccga gctgaaacgt
      841 gtcgcgccca tcgagccaga agtgcagtcc ggcatcgcaa ctgagcgagg tgagtgggat
      901 caccacaaca cagatgaccc aggcaaagcg ctcggaacgg atctgcagcg actgctgctg
      961 cagcgcggag aagaatcctg aactgaacag cacgaatgtg gcttcgacca ggccgaagtg
     1021 attgcgcgcg tggtgctcca catggcgaag cgccagcatg caggccaaac cgcgcagcgc
     1081 cgcggccatc agcgtgacct tccagttcca gtgcgtgatg gcgtagaacc accctgaacc
     1141 cggcatcgca cctgtcccga attttccgcc gtccctcatg ctctcgagcc ttcgttcccc
     1201 gtccggattg gcttccgctc cctcaacgcc accttcccat cgcctcaggc catgcaccat
     1261 caatggggca ttacagttcg cacaatttca ccgcgtcggc actccattcg ttacgaaccg
     1321 cgacgttgct atctcccgat agttaccaac gattgcgagt tttttcgaaa cttcacccca
     1381 acccatgggt tcacccttgc gacggcgaac ttcgccgctc catcatcaag acccacgcag
     1441 ttcacgggca gcttctcttc tgcatcgggc tacttctccg aggatcccag cctggctgca
     1501 cccggtttcc gcgctttttg cgcgcatact gcttctgcat ccaggccgtg atctgtcgat
     1561 agcaactcga tgcatgaaac agactccccg gaaccgcaga aagcgattcc cgatgtccac
     1621 ccacgcccgc atccgctccg caattgccgc cctttgcctg agtgccgctc cgcttctgct
     1681 cgccctcttc cttctggccg gctgcggcat gcagtccgga aactccgtct ccaatcctgg
     1741 aaccacttcc ggttctgcct tcgtcatcgg caccgacgcg ccgctggcca gcgtcgtctc
     1801 atttaacgcc acccttcagg cgatcaacgc gattgacgcc agtaacaact cggtcgccct
     1861 catcagcggt tcgcccaccg tcgacttcgc tcgcttcaac ggcctgcaga ccctgctcga
     1921 tatgaacagc gtgcccgccg gcacgtatac gcaaatccag gtcgtcttct ccagcgccac
     1981 cattggctat ctgcagaccg gctccggcgc gcccaccatc cagaccatgc ccgccacctt
     2041 caccaccaat acggtgacga aaacgctggc caatccgctg gtcgtttcaa ccgccgggcc
     2101 tgtgggcatt cacatggatt tccgcctcga taagtccgtt caggtctcga atggccagat
     2161 caccggcgac gtgaatccca ccctcgacat ccgcgccgtg ggcccgactg atcccgatgc
     2221 ttacgtcgac gaattcatcg ccggcgtcgt caaccccaca gcctcctcgc agtcgttcac
     2281 cattcaggga ccccacggcc gcaccttcac cgtgaacgtg aatggacaga ccgagtggga
     2341 caacggcgaa aagctgagcg acctgacctc caccagtgtt gtgcagattt cgggcagcct
     2401 cgatcgggct gactccacca tcgatgccga cgaagtcgcc atcctgacgc agaacggctt
     2461 ctacgccggc ggccagatca cctatgtcca gccctccagc ggtccggcga ccgccttcca
     2521 gctctatgtc cgcggcaccc tgccggcgag cggcgatggt gtctcgctgg gccagatcgc
     2581 gcaggtcaac ctcagcggct cagagaagtt cttcatctac tggatgcaca acccgctcac
     2641 ggaattcctc ttcaactcct caacgctgct gccgggtcaa catgtcaccg taggcggagc
     2701 gctgtctggc gcggccgacg gcaatgcgct cagcgtcaag cgagtcgtcc tgcggaactg
     2761 gggcttcaac ggaacgctgg tgcccaattc catctccaac ggcacattcc agatgaacat
     2821 cgccggcttt gcgggcctgc tggtaccgaa tcagccggtg acggtgtaca caggtccagg
     2881 cacgcggttc cgcctcggcc tgaatgcgat ggcggatgtg agcacagcaa caaagatccg
     2941 cgtcgtcggg ctgctgatca aggaccccac ctccgggaac tccgtgctgc tggcccacta
     3001 catcgacgac ttggacaagg actagactcc tccccggcga acccacgaaa ggcggcgc
//
LOCUS       DASZYN010000724         5540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1138834, whole genome shotgun sequence.
ACCESSION   DASZYN010000724 DASZYN010000000
VERSION     DASZYN010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138834"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VHE33_18580"
     CDS             <1..838
                     /locus_tag="VHE33_18580"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: protein motif:HMM:NF008808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HVT99512.1"
                     /translation="EALKARIEPYIAEGGAAVGDRGAMEAFLELRLALEAGLVRAAEP
                     DAASPTGWRVNAWVKRGILLGFRIGQLQGSGETLSFVDKDTYPARRFAPEQGIRIVPG
                     GSSVRAGAWLGRSVVMMPPSYVNVGAYVDEGSMIDSHALVGSCAQIGKRVHLSAAAQV
                     GGVLEPVNASPVILEDDVLVGGNCGVYEGTIVRSKAVLAAGTILTRGTPLYDAVRGEV
                     LRADADMPLIVPEGAVVVPGARAVSKGKGKEMGLSVYTPVIVKYRDDKTELSLALEDL
                     LR"
     gene            complement(850..1407)
                     /locus_tag="VHE33_18585"
     CDS             complement(850..1407)
                     /locus_tag="VHE33_18585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99513.1"
                     /translation="MIRRAVRFLGNVVSGSPNEEEDPIPSLVLLLEQPVPLTRSAALE
                     LAVKAWGESQNEASIAPRRRRGRWLVRVSDVPFWMRGGRAPYRRQGHETNEVRQGAWD
                     RHRAWLQVDYPAGAKMSDSELPACYKALFLMANYLWGENCLGLYLPVQDVTIPNMGDL
                     IASIRWAAKNGTPLSFLHEREEKEA"
     gene            complement(1580..1852)
                     /locus_tag="VHE33_18590"
     CDS             complement(1580..1852)
                     /locus_tag="VHE33_18590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99514.1"
                     /translation="MVWDVSFTCDICGKKKGEANHWWMYMLGDVPCFEEGQPGQRFTL
                     LPWNFAESRNPDMHHLCGQGCAMQAMERFMTNRSVEREPRAISEVA"
     gene            1973..2167
                     /locus_tag="VHE33_18595"
     CDS             1973..2167
                     /locus_tag="VHE33_18595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99515.1"
                     /translation="MVCTRCHATEFRLSSLRFRDLEHLAVLQYPMRCRYCHHRTYGNF
                     LLALMLFQLRRHHFGHHENA"
     gene            complement(2431..5097)
                     /locus_tag="VHE33_18600"
     CDS             complement(2431..5097)
                     /locus_tag="VHE33_18600"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HVT99516.1"
                     /translation="MNRPHFSRVLPLVLLTLAVAPLARAASEPPTRATLPNGMRVVII
                     RNTLAPVVTVQANVLAGGNETPEGFPGMAHALEHMAFRGCKGMTADQTAAIFALMGGD
                     NNADTQQNITQFFETVPAADVNIALQAEAACLSGIDNSDAEWQHERGAIEQEVARDLS
                     NPTYKFLTRLNEDVFAGTPYAHDALGTRPSFEKTTGAMLKEYADKWYTPSNVILVVVG
                     DVDPAATLEKIRNLYSDIPSHPVPAHPQVHLQPVKSDTFTLDSNLPYVLGFIAWRMPG
                     TDSPDWAALQILSDVLASQRADIYGMVPAGKALAAEFGLGENYTEASVAFGLVALPAG
                     QDANPPLDELRGIVNKYAANGVPADLVDAAKRAEIAQAEFQRNSIPDLASVWSQALAG
                     EGRNSPDEDVEAIKKVTLADVNRVARQYLLNAPTITATLKPAPNGEPVAQKGFGGSEK
                     VTSAPTKPVTLPAWAEKELSQLQVPTTFIPVSDTKLANGIRLIVRTDTTSPTVTVMGS
                     VKHNGDLQSPPEKEGVGEVLDQLYSYGTTTLDRIAFQKALDDIAANESAGYDFSVKVL
                     KEYFSRGVELLADNELHPALPEQAFKITQQQVAQFVAGNLQSPGYRTSKALDVALLPK
                     NDPVLREATPQTIMKLTLDDVKQYHAATVRPDLTTIVVIGDVTPEEAKTVIEKSFGDW
                     KSTGPAPNTTLPAIPVNKASAANVPDAQAVQDSVILAEQIDINRFDPDYYPLQLGNHV
                     LGGGFYATRLYHDLRQVAGYVYNVNVSMDASKTRTSYSVDYGCDPPNVSKARALIVRD
                     LDQMRTQDVSDAELHQAKALLLRQIPLSESSEEAVAGGLLGRAEVGLPLDEPIRAAKK
                     YYALTAADVKAAFAKHIQPDNLVQVVRGPTPQ"
     gene            complement(5199..>5540)
                     /locus_tag="VHE33_18605"
     CDS             complement(5199..>5540)
                     /locus_tag="VHE33_18605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99517.1"
                     /translation="DVIDATLKSTGTGDAGGLAAAVKRGVDSRIVEAMLGLAANSEAS
                     PEVRGILRVKLQSLRDRYVRDSQGGGADSAFGSFEAARIDEFLRDPAKFVPAKPIAAP
                     PGMPIGDDQQD"
BASE COUNT          977 a         1628 c         1789 g         1146 t
ORIGIN      
        1 cgaagctctc aaagcaagaa ttgaacctta cattgccgaa ggtggcgccg cggttggcga
       61 tcgaggcgcg atggaagcct tcctggaatt gcgcctcgcg ctggaagctg gactggtgcg
      121 cgcagcggag cccgacgctg catccccaac cggttggcgt gtgaatgcct gggtcaagcg
      181 ggggatcctg ctcggctttc gcatcgggca actgcagggg tccggcgaaa ctctctcctt
      241 cgtcgataag gacacttatc cggcgcggcg attcgcgcct gaacagggca tccgcatcgt
      301 tcccggcggt tcgtcggttc gtgcgggagc ctggcttggg cgcagcgttg tgatgatgcc
      361 gcccagctac gtgaacgtgg gcgcgtatgt ggatgaaggc agcatgatcg actcgcatgc
      421 gctggtgggc agctgcgcgc agatcggtaa gcgcgtgcat ctgagcgccg ccgcgcaggt
      481 gggtggggtg ctcgagccgg tcaacgcgag tcccgtcatt ctcgaagacg atgtactggt
      541 gggcggcaac tgcggcgtgt acgaaggcac gattgttcgc agcaaggcgg tgctggcggc
      601 gggaaccatc ctgacccgcg gcacgccgct ctacgatgcc gtgcggggcg aagtgctgcg
      661 cgccgatgcc gatatgccgc tcatcgttcc ggaaggcgcg gtggtggtcc ccggcgctcg
      721 ggccgtgagc aaagggaagg gcaaggagat ggggctcagc gtctatacgc ccgtgatcgt
      781 gaagtatcgc gacgacaaga cggagctgag tttggcgctg gaagatctgc tgcgctgatc
      841 gaaccggttc tacgcttcct tctcttcgcg ttcatggagg aaggagagcg gggtaccatt
      901 tttcgcggcc cagcggatgc tggcgatgag gtcgcccatg ttgggaatgg ttacatcctg
      961 cacaggcaga tatagaccga gacagttctc tccccacaga tagttcgcca tgagaaacag
     1021 agccttgtaa caggcgggca actccgagtc ggacatcttt gcgccggcgg gatagtcgac
     1081 ctgcagccag gctcggtggc gatcccaggc accctgccgg acctcattgg tctcgtgtcc
     1141 ctggcgccga tacggggctc ggccgccacg catccagaac ggcacgtcgg agacacgaac
     1201 cagccatcgt ccacggcgcc gccgcggcgc gatcgaggct tcgttctggg attcgcccca
     1261 ggcttttacc gccagctcaa gtgctgcgga cctcgtcagc ggcaccggct gttccagcag
     1321 cagcaccagc gacgggatag gatcctcttc ttcgttcggg gacccactga ccacatttcc
     1381 aagaaagcgg accgctcttc ggatcatgcg ctatccatgg tacgacgagc gtatcgtacg
     1441 ggaaaggtaa taatgatcag atagccatgt tgattaacta ccgatgtaac tggataactg
     1501 atgacttaag aagaagatga tctgcggcgc gcctggcgtt gtttttttga gcgcttcgcg
     1561 gttctatcca ggcaggaaat caggcgactt cactgatcgc gcgcggctca cgctcgaccg
     1621 agcgattcgt cataaaccgc tccatggcct gcattgcgca gccctggccg caaaggtggt
     1681 gcatgtccgg attgcggctc tcggcaaagt tccatggcag gagggtgaag cgctggccgg
     1741 gctgcccctc ttcaaaacag gggacatcgc ccagcatata catccaccag tggttggctt
     1801 cgcccttctt cttaccgcag atgtcgcacg tgaaagatac atcccaaacc attggttact
     1861 ccctgaccaa tatgcttttc gcaacagtac tacgaaagtg ggaggggggc agtagccctt
     1921 ccgggggagg gctctggatt tctcgtcccc atcctcgtat ggtgatcgtg ccatggtttg
     1981 cacgcggtgt cacgcgaccg aatttcgcct ttccagcctg cgctttcgcg acctggagca
     2041 cctggctgtg ctgcaatatc ccatgcgctg ccgttactgt catcaccgca cctacggaaa
     2101 tttcctgctg gccctgatgc tgttccagtt gcgccggcac catttcggcc accacgaaaa
     2161 cgcgtaaaag cacgctgtcg ggctgtgttg tggatgcctg gatgcgacat ttcgccgctg
     2221 tgtttggccg cttaaccgtg ttttggattg cgcggcatgg tttggcgcgt tctgacaacc
     2281 ggcacggtga tagagaccga tttggtggtc tgggactgcc gcgttttcca gggacgtgcg
     2341 acctgcggat ggcgaggctt atccataaga acctcccctg ccgggttcag tttagtcctc
     2401 gccgcccgtt tgcaacagcc gctccgctgc ttactgtggt gtcgggccgc gcacgacctg
     2461 caccaggttg tcaggctgaa tgtgtttcgc gaaagccgct ttcacatccg cagcggtcag
     2521 ggcatagtac ttcttcgcgg cacggatggg ttcatccagg ggcaaaccga cctcggcccg
     2581 gccgagcagc ccgccggcga ccgcctcttc gctcgattcg ctcagcggaa tctgccggag
     2641 caggagcgct ttggcctgat gcagctccgc atcggagaca tcctgggtcc gcatctgatc
     2701 caggtcgcgc acaatgagcg cccgcgcctt cgagacgttg ggcggatcgc atccataatc
     2761 gaccgagtag ctggtgcggg tcttcgacgc atccatgctg acgttcacgt tgtagacata
     2821 tccggcgacc tggcgaagat cgtgatagag gcgcgtggca tagaagccgc cgccgagcac
     2881 gtgattgccg agttgcaggg gatagtagtc cgggtcgaag cggttgatgt cgatctgctc
     2941 ggccaggatc acggaatcct gcacggcctg cgcatcggga acgttggctg cggaagcctt
     3001 gttcacgggg atcgcgggga gcgtggtgtt gggcgcgggt ccggtggact tccagtcgcc
     3061 aaacgatttc tcgatgaccg tcttcgcctc ttccggggtt acatctccaa tcacgacgat
     3121 ggtggtgagg tcggggcgaa cggtggcggc gtggtactgt tttacgtcgt cgagggtcag
     3181 cttcatgatc gtctgcggcg tcgcctcgcg cagcaccgga tcgttcttcg gcagcagcgc
     3241 cacatcaagc gctttcgacg tgcggtagcc agggctctgc agattgccgg cgacaaattg
     3301 tgcgacctgc tgctgcgtga ttttgaaggc ctgctccggc aacgccggat gcagctcgtt
     3361 atccgcgagc agctcgacgc cgcgcgagaa atactccttc agcaccttca ctgaaaagtc
     3421 atagcctgcg ctttcgttgg ccgcgatgtc atcgagagcc ttttgaaatg cgatacggtc
     3481 gagggtcgtc gttccgtagg agtaaagctg gtcgaggacc tcgccaacgc cctccttctc
     3541 gggaggcgac tgcaggtcgc cattatgttt caccgaaccc atcacggtaa cggtcgggct
     3601 ggtggtgtcg gtgcggacga tcaggcggat gccattcgcg agttttgtat ccgaaacagg
     3661 aataaaagtg gtgggcacct gcagctggct gagttccttc tccgcccagg ccggcagggt
     3721 cacgggcttg gtgggcgccg aggtcacctt ctcgctgccg ccgaagcctt tttgcgcaac
     3781 cggctcgccg ttcggtgcgg gcttgagcgt ggctgtaatc gttggtgcat tcagcaggta
     3841 ttgcctggcc actcgattca catcggccag cgtgactttt ttaatggcct cgacatcttc
     3901 gtccggcgag ttgcggcctt cgccggcaag ggcctgggac cagactgagg cgagatcagg
     3961 aatggagttg cgctggaact cagcctgcgc gatttcggcg cgcttcgccg catcgaccag
     4021 atcggcggga acgccgttcg ccgcgtattt gttcacgatc ccgcgcagct cgtccagagg
     4081 cggattggcg tcctgaccgg caggcagcgc gaccaatcca aaagcgacgc tcgcttcggt
     4141 gtagttttcg cccaaaccaa attcggcggc gagggccttg cccgcgggca ccattccgta
     4201 aatgtcggcg cgctggctcg ccaggacgtc ggagagaatc tgcagtgccg cccagtcggg
     4261 cgagtcggtg cccggcatcc gccacgcgat gaatcccaga acgtaaggca gattgctgtc
     4321 gagggtgaag gtatcggact ttaccggctg gaggtgaacc tgaggatgcg ccgggaccgg
     4381 atggctggga atgtcactgt agaggttacg aattttttca agtgtcgccg ccggatcgac
     4441 atcgcctacg accacgagaa tgacgttgga gggggtgtac cacttgtcgg cgtattcctt
     4501 gagcatcgct cctgtggttt tttcaaacga ggggcgcgta cccagggcgt cgtgcgcgta
     4561 gggggtgccg gcgaacacgt cctcgttcag gcgggtcaga aatttgtagg ttggattgga
     4621 gagatcgcgc gccacttcct gttcaatcgc gccgcgttcg tgctgccact ccgcgtcgga
     4681 gttgtcgatg ccgctcaggc aggctgcttc cgcctggaga gctatgttca catccgctgc
     4741 gggtactgtt tcgaaaaatt gcgtgatgtt ttgttgcgta tcggcgttgt tgtcgccgcc
     4801 catgagcgcg aagatggctg ccgtttggtc ggccgtcatc cccttgcaac cgcggaaggc
     4861 catgtgctcc agagcgtgcg ccataccggg gaaaccctcg ggcgtctcat tgccgccggc
     4921 aaggacattg gcctgcaccg tgaccacggg cgcgagggtg ttgcgaatga tcacgacgcg
     4981 catcccgttg ggcagcgttg cccgggtggg cggttcactc gcggcgcgcg ccagcggcgc
     5041 aacggcaagg gtcagcagga caagcggcaa aacgcgcgaa aaatgcggac gattcacgat
     5101 ccctccggga cgggtttccc ttcaggaatg gtaagggaaa ggcgcgcgta cggcctgccg
     5161 catgtgggcc ttagtgacaa actgcgtgtt tgcggcgatc agtcctgctg gtcgtcgccg
     5221 atcggcatgc cgggcggtgc ggcaatgggc ttcgcgggga cgaatttggc gggatccctg
     5281 aggaactcgt cgatgcgggc agcctcgaag ctcccgaacg ctgaatccgc gccgccgccc
     5341 tgggagtcac gcacataccg atcgcgcagc gactgcagct tcacgcgcag gatgcccctc
     5401 acttccggcg acgcctccga gttggctgcg aggcccagca tggcttcgac gatgcggcta
     5461 tccacaccgc gcttcacggc tgcggcgagt ccgcctgcat ccccagtccc cgtcgatttc
     5521 agggtcgcgt cgatgacatc
//
LOCUS       DASZYN010000725         3958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1011078, whole genome shotgun sequence.
ACCESSION   DASZYN010000725 DASZYN010000000
VERSION     DASZYN010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011078"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VHE33_18610"
     CDS             complement(<1..611)
                     /locus_tag="VHE33_18610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99518.1"
                     /translation="MRSGFENKRNVIILVVLAVVLMGAIVYFVRYVFGGSPSPAPAPA
                     PSSENRGASPETAQTQTEARDANGHAAKKMPPLEKLDPTLHPEVMAGAESLEYSGKGR
                     NIFSMTSAPVEIPKVIATVRPGPVVPQAPPGPPPPPPIDLKFFGYSANNGARKAFLLH
                     GDDVFIANEGDVVDHHYKVVKISPASIQVTDLLYNNTQTLPLT"
     gene            complement(608..1228)
                     /locus_tag="VHE33_18615"
     CDS             complement(608..1228)
                     /locus_tag="VHE33_18615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99519.1"
                     /translation="MNRVTKLNLHFVLLALVVAADVFIGVRYALAWRSINSVRSAEFI
                     QENVRLAQLEAQMKHLSGLPEKVNAADEAAQKFYDMRLAPNYSTVLSQLGAVASKSNV
                     KLSRAAYVQSDAPSGLTELHIDAGLSGQYSDLMHFINGLERDQDHVFFIINGVTLNGQ
                     QGGLVNLRLRLTTWLHGGATDLPTGFDNNLEPAPSEPQPAGGEGPQ"
     gene            complement(1225..1950)
                     /locus_tag="VHE33_18620"
     CDS             complement(1225..1950)
                     /locus_tag="VHE33_18620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99520.1"
                     /translation="MKIAVNLASRPYIDLRPVYGRLRTWIVILALVGGALWYLDRNEA
                     DEAQAARAQVATVESRVRDLEKQQQGYRRMMQQPKDAGILQQSEFLNSLFQRKAFSWT
                     ATMTDLETVLPTGVQVLAIDPITGPGGNVIIRLRVTGERDRGLALVRNLEKSRHFAAP
                     RLAAESLATAQGGAGVQNASAGNLVNFDILADYRPLPLQAGSEEKSASAASKQPAAVT
                     HPKTRAVKEQKKPSGATGGRKTP"
     gene            complement(1951..2835)
                     /locus_tag="VHE33_18625"
     CDS             complement(1951..2835)
                     /locus_tag="VHE33_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99521.1"
                     /translation="MPGTRPKAACEIRSEGVTAARSGKKHGEIVTAFAPLPEGAVTPG
                     LKVPNFPDAHAVVAALESALDSVSPRSKELTLVFPDAAARVLLLDFDTLPAKRQEALS
                     VVRFRLRKMVPFDVETAAVSYQVMAERNGQLNVLVTVMPADVVAEYESAVRDAGYEPG
                     ALLPSTLAAAAAMEGDGAQLMMNHSRASVTTAVTQGDEMLLHRSMDLPAEPQAREEEM
                     AQAVITALAWYEDTLKRLPSTMFYAGPGGADAAQQTRWAALVQPAPRLVDLTPADASA
                     MTSVPAGVVAGVTGALAT"
     gene            complement(2996..>3958)
                     /locus_tag="VHE33_18630"
     CDS             complement(2996..>3958)
                     /locus_tag="VHE33_18630"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HVT99522.1"
                     /translation="DGRFRVRYKGRLIDFRVSIMPTVHGENAVLRVLDKESMSEKFRK
                     LTLDVVGFADDDVKRFRRYIREPYGMVLVTGPTGSGKTTTLYAALNEIKSDEDKIITI
                     EDPVEYQIRGITQIPVNEKKGLTFARGLRSILRHDPDKILVGEIRDQETAQIAINSAL
                     TGHLVFTTVHANNVVDVLGRFVNMGVEVYNFVSALNCILAQRLVRTICEYCVQEVRYD
                     DDLLIDSGLDLREWRNFPFREGRGCIECAGTGYRGRTAIHELLDLTDPIREIILEKKP
                     SSEVRKLAQQEGMHFLRESALDRVRRGLTTLKEINKVTFIEASR"
BASE COUNT          697 a         1425 c         1079 g          757 t
ORIGIN      
        1 tgtgtcagcg gcaacgtctg cgtgttgttg tacagcagat ccgttacctg gatcgacgcc
       61 ggcgaaatct tcaccacctt atagtgatga tccaccacgt ccccttcgtt ggcgatgaac
      121 acatcgtcgc cgtgcagcag aaaggccttc ctcgcgccat tgttggccga gtatccaaaa
      181 aacttcagat cgatcggcgg aggaggcggc ggacccggag gcgcctgcgg cactaccggt
      241 cccgggcgca cagtcgcaat cactttcgga atctccaccg gcgccgatgt catcgagaaa
      301 atattccgcc ccttgccgct gtactccagc gactccgcac ctgccatcac ttccggatgc
      361 agcgtcggat ccagcttctc cagcggaggc attttcttcg ccgcgtgacc attcgcatcg
      421 cgtgcctctg tctgcgtctg cgccgtctcc ggcgacgccc cgcggttctc cgatgacgga
      481 gccggcgctg gcgccggaga agggctgccg ccgaaaacat accgcacgaa atacacgatg
      541 gcgcccatca gcaccaccgc cagcaccacc aggatgatga cgttccgctt gttttcgaag
      601 ccgctcctca ttgcggtccc tcgccccccg caggctgcgg ctcgctcggc gccggctcca
      661 aattgttgtc gaatccggtg ggcagatcgg tggccccgcc atgcagccag gtcgtcaggc
      721 gcaggcgcag gttcaccagg ccgccctgct gcccgttcag cgtgacgccg ttgatgatga
      781 agaagacgtg gtcctgatcg cgttccagtc cgttgatgaa gtgcatcagg tccgagtact
      841 ggccgcttag gcccgcatcg atgtgcagtt ccgtcagccc gctcggtgcg tcgctctgca
      901 catacgccgc ccgcgagagc ttcacgttgc tcttcgaggc caccgccccc agttggctca
      961 ggacggtcga atagttcggc gcgaggcgca tgtcgtagaa cttctgcgcg gcctcatccg
     1021 cggcgttcac cttttccggc aggccgctca ggtgcttcat ctgcgcctcg agctgcgcca
     1081 gccgcacgtt ctcctgaata aattccgccg agcgcaccga attgatcgat cgccacgcaa
     1141 gcgcgtaacg cacgccgata aaaacatcgg ccgctacgac cagagccagc agcacgaaat
     1201 gcagattcag cttcgtcacg cgattcatgg agttttacgc cctcccgtcg cgccgctcgg
     1261 cttcttctgc tctttcactg cccgagtctt cggatgagta acggccgccg gctgcttcga
     1321 ggccgcactc gcggatttct cctcgctccc agcctgcagc ggcagggggc gataatccgc
     1381 cagaatgtcg aaattcacca ggttccccgc gctcgcattc tgcacacccg caccgccctg
     1441 cgctgtagcc agggactccg ccgccaggcg cggcgccgca aaatgccgtg acttctccag
     1501 gttccgaacc agcgccagtc cgcgatcgcg ctcacccgtc acacgcagcc ggatgatcac
     1561 attgcctccg ggacccgtaa tcggatcgat ggccagcacc tgcacgccgg tcggcagcac
     1621 cgtctccaga tccgtcatcg tcgccgtcca cgaaaacgcc ttgcgctgga acagcgaatt
     1681 caggaattcc gactgctgca ggatgcccgc gtccttcggc tgctgcatca tgcgccgata
     1741 tccctgctgc tgcttctcca gatcgcgcac acggctttct accgttgcca cctgcgcacg
     1801 cgccgcctgc gcttcgtcgg cctcgttgcg atccaggtac cacagcgccc cacccaccag
     1861 agcaaggatc acgatccacg tgcgcagccg cccgtacacc ggccgcaggt cgatgtaagg
     1921 acggctcgcg agattaacag caatcttcat ttaagtcgcc agcgcccccg ttacccccgc
     1981 caccacaccc gccggcacac tcgtcatggc cgaagcatcg gcgggagtca gatccaccag
     2041 cctcggcgcc ggctgcacca gcgctgccca tcgcgtctgc tgggccgcat ccgcgcctcc
     2101 cggacccgca tagaacatcg tcgatggcag cctcttcagc gtgtcttcat accacgccag
     2161 tgcggtgatc accgcctgcg ccatctcctc ctcgcgcgcc tgcggctccg caggcagatc
     2221 catggagcgg tgcagcagca tctcatcgcc ttgtgtaacc gccgtggtta cagaagcgcg
     2281 gctgtgattc atcatcagct gcgcgccgtc gccttccatg gctgcggcgg ccgccagcgt
     2341 gctcggcagc aacgcacccg gctcatagcc ggcatcccga accgcgcttt cgtactccgc
     2401 caccacatcg gccggcatca ccgtcaccag cacgttcagc tggccatttc tctccgccat
     2461 cacctgatag ctcaccgccg ccgtttccac gtcgaacggc accatcttgc gcagccggaa
     2521 acgcaccacc gacaatgctt cctggcgctt ggccggcagc gtgtcgaaat cgagcagcag
     2581 gacgcgcgcc gccgcgtccg gaaacaccag cgtcagctcc ttgctgcgcg ggctcacgct
     2641 gtccagggcc gattccagcg ccgccaccac cgcgtgagcg tcaggaaaat tgggcacctt
     2701 cagacccggc gtcaccgcgc cttccggcag cggcgcaaac gccgtgacga tttcgccgtg
     2761 cttcttgccc gaacgcgccg cggtcactcc ctcggagcgg atctcgcaag ccgccttggg
     2821 acgggttccc ggcaccggtt taaagaaatt ctccagattc ggcactaacg cgatgcctcg
     2881 accttcgcga ccaaccctgt catcctgagc gcagcgaagg atctgctttc atgaattttg
     2941 tccggcgcgc aggcgccgat cagctgtatt ctttcggacc cgaggaggca gcgcactacc
     3001 gcgatgcctc gatgaacgtc accttgttaa tttccttcag cgtcgtcagc ccgcgccgca
     3061 cgcggtccag cgccgactcg cgcaggaagt gcatgccttc ctgctgcgcc agcttccgca
     3121 cttcggagct gggcttcttc tccagaatga tttcacgaat cggatcggtc aaatccagca
     3181 gctcgtggat cgccgtccgc ccgcgatacc ccgttcccgc gcactcaatg cagccgcgcc
     3241 cttcgcggaa ggggaagttc cgccattccc tcagatccag cccgctgtcg atcagaaggt
     3301 cgtcgtcgta gcgaacctcc tgcacgcaat actcgcagat ggtgcgcacc agcctctgcg
     3361 ccagaatgca gttcagcgcc gagacgaagt tgtagacctc cacgcccata ttcacgaaac
     3421 gtcccagcac gtccaccacg ttgttcgcgt gcaccgtggt gaacaccagg tggccggtca
     3481 gcgccgagtt aatggcgatt tgcgccgttt cctggtcgcg gatttcgccg accagaatct
     3541 tgtccggatc gtggcgcagg atcgagcgca gtccgcgcgc gaacgtcagc cccttctttt
     3601 cgttcaccgg aatctgcgtg atgccgcgaa tctgatactc gaccgggtcc tcaatcgtga
     3661 tgatcttgtc ttcgtcgctc ttgatctcgt tcagcgccgc ataaagagtc gtggtcttgc
     3721 ccgaacccgt cggtcccgtc accagcacca tgccgtacgg ctcgcgaata taccgccgga
     3781 accgcttcac atcgtcgtcc gcaaagccca ccacgtccag tgtcagcttg cggaacttct
     3841 cgctcatcga ttccttgtcc agcacgcgca gcacggcatt ttcaccatgc acggtcggca
     3901 tgatcgagac gcgaaagtcg atcagccgcc ccttgtagcg gacccggaaa cggccgtc
//
LOCUS       DASZYN010000726        10643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_174604, whole genome shotgun sequence.
ACCESSION   DASZYN010000726 DASZYN010000000
VERSION     DASZYN010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 10643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10643
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174604"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VHE33_18635"
     CDS             complement(<1..571)
                     /locus_tag="VHE33_18635"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HVT99523.1"
                     /translation="MTRKFTGALLSEAVAMFIIIAFGDSVAAMYSLYDPSPYQHAYWG
                     VCIAWGLAVAVAIYTTGSISGCHANPAVTLALALYRKFSWKQVVPYCAAQILGGFAGA
                     ALVLQLFSPVINEFNLTHHLTRAAGGAAGVFFTHPAPGITPLHAFGDEIVLTAFLIFG
                     IFAITEQYNEMAPGANFGALMIGFLVALIG"
     gene            complement(680..1900)
                     /locus_tag="VHE33_18640"
     CDS             complement(680..1900)
                     /locus_tag="VHE33_18640"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT99524.1"
                     /translation="MNRVPLLRVLVAAVVAAACLRGSAQTTVSYAQQRDAAITLQQQG
                     ELAQAEVAWQTILKSHPADADACANLGVISSQQKHYGDAVRYYRKALAIKPSMPGLQM
                     NLGLALFKSGDMKGSARVFVSLYHRTSPDSPDGQRLRLLIGMADYGAGDYAAAIPYLR
                     DATTRDPQNLPYRLVLAHSCLWARKYKCVLDVYHEILALNAESAEADMLAGEAYDEMR
                     DHESAIQQFRNAVKADPKIPGVHFGLGYLLWAQSQYEEAAEQFGLEVENQPNDAESIA
                     YWADSNVRLNRADVARPLVEKALKLNSRQELPWLDLGILDAQAGNNDAAFRDFQTAAK
                     IAPDDVQVHWRLARLYQSMGKRVEAQAEFAKTKRLTQAADQSLRQKLQASHAQDSQSE
                     APSGSAAASSPSQP"
     gene            complement(2015..5533)
                     /locus_tag="VHE33_18645"
     CDS             complement(2015..5533)
                     /locus_tag="VHE33_18645"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT99525.1"
                     /translation="MIAIARPAPFSTRIHAIRRLFSVSFAVLALVFALSSTLCAQENA
                     TINGDCTDSTGAVVANASITLTNTATGVERKQVSNSVGEFRFANLAIGTYTMTATAPG
                     FETFTKTGIVVNVAQTVAADIVFKVGSQTQTVSVQANALQTQTETSEVSTLISSEQVE
                     QLSTNGRNVTQLAALGLGVSSTLPSFGGVDALTSANSISFNGTRNTHNVYLIDGGEQN
                     DRGCGGCFMNLPAQDAIAEFQTLDSNYPPDYGIGSGGTIVMALKSGGHDFHGELYEFN
                     RNTAYNANDYFVKRRATGNSRPIFQLNEPGGNIGGPLFIPHVYNENRNRTFFFWNEEW
                     RRLILGSTPLTANTIPAANFPTAGTNLTYVPWNNPTTASPACNAGVQAPCVPKTTDPA
                     KLALYAQDGLTAGERFPGNVIPANLMDPNAVLELNSGTFPKPNLGTSQYTASIPQPDN
                     IREDVVRIDHTFSSKYQIMGHYLHDAMAKQFYPPLWGNSTYPTVGTAMTNPSYTAVIR
                     LTQTYSPSLLNETGFFYAGNKIGLTPIAGKSGGSSFVQPSGWSAVSLFPTANNRMARM
                     PEIQLQGTPINTTWSPSYFPWKNGYEGFQYKDDLSWTKGRHQFKFGFSWLHAYKNQEL
                     QANTNGTAIFNTSAFSGDSYVNFLLGDTANFSQLEYLAGKHWVTNTYSGYGMDNWHVN
                     RNLTLNLGIRYDDLPHTFERYDAFSNFVIADYDTSKGNPVDANGRLITSQLSTFSKTG
                     NELFYLNGIREAGVGGFPRGNVENFHFTWQPRVGFAYNIRGDGKTVLRGGYGVFFERV
                     QGNDVYNAALNPPFAYQPSADSVYFSDPHTSALNGQTAAQQYFPSNLTNIKYHYPPPG
                     TSNYSLGIQREVAPSVVAVVQYVGSNGWDQNNDRQINTLPVGDITHRQAVAGGAPANP
                     YRNFPGFANINQEENETNFHYNALQTGIRFENKHGLTSQLAYTWSHEIDTATNDLNGL
                     SNPYNTNYDRASGGLDRRQIFNASFVYGLPFARHSDNLFAREVVGGWTVSGIALFESG
                     IPQVVRYSGGDTVGLGGGTTNRPNQTGKMSYPKKQTAWFDKSKFTDPVAPWNGGPTGV
                     NAGFGNAGRDAVTLPGLENWNLSLFKSIPFKEGGPRIELRFESFNTFNHTQFQNLDLN
                     NKDGNFGQTTGIYQPRALELGGKFIF"
     gene            6271..8841
                     /locus_tag="VHE33_18650"
     CDS             6271..8841
                     /locus_tag="VHE33_18650"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HVT99526.1"
                     /translation="MNSNLSPEERARDLVSHMTLEEKASQLVNQARAIPRLNVPAYDW
                     WSEALHGVAVNGTTEFPEPMGLAATFDAPRIHEMAIDIGIEGRIKHMQAERAGHSNIM
                     EGLDFWAPNLNIFRDPRWGRGQETYGEDPYLTGRMGVAFVTGMQGDDPKYYRVIATPK
                     HYAVHSGPEPTRHMADVDVSKHDEMDTYLPAFRAAVTEGKADSVMCAYNGIKGQPACA
                     NQFLLEDQLRKHWGFKGYVVSDCGAVIDIYAGHHYRPTQAQASGISLLRGMDNECADF
                     FAKVHDDHDYKPYIDAVRQGYLSENDVDTALVRLFTARMKLGMFDPPDMVPYTKIDEK
                     ELDSAEHRALARKLANESMVLLKNDGTLPLKSSVTKIAVIGPLADQNRVLMGNYSGMP
                     THSVTVLDGLKAEFPNAKIRFVPGTQFLRNDGDPLPNSLLTTPDGKPGLKAEYGSIEG
                     MDLNPRSKPKPLTTRTEPNVNLAAGSLPAEAQGKPAISVEWSGFLTAPDTGDYLLGIT
                     VDGFGRLMVDGKPFAQVFSMGGRNAKMGRIHLERGEKVPITVTYAKLNPAGEPKAQLL
                     WGRYNPAPSPEAVAAAKEADVTIAVVGITSELEGEEMPVSEPGFLGGDRTSLDLPQPE
                     ENLIETVAATGKPLVVVLMNGSALSVNWAKAHANAILEAWYSGEEGGAAIAETLSGKN
                     NPAGRLPVTFYTGVDQLPNFEDYSMNNRTYRYFKGEPLYPFGYGLSYTKFSYSGLEVP
                     AEEVNAGDPVHATVTVTNTGSLAGDEVVELYLQFPDVPGAPLRALRGFQRVHLEPGAS
                     QKVDFTLGRRDLSMVTELGDPIIADGAYTITIGGGQPNTEAPVATGHFRVKGQLMLPE
                     "
     gene            9064..>10643
                     /locus_tag="VHE33_18655"
     CDS             9064..>10643
                     /locus_tag="VHE33_18655"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HVT99527.1"
                     /translation="MRMQRFAILSIVAMFVFTGPVFLGQSAGPAVITIHTDQTASKVS
                     PTLYGLMTEEINHSYEGGLYAELIQNDTFRSDWSGVNEWILVQNGNASAKMGIDKTTG
                     PSEALPYSLHLEVSEASASGSGGILNGGFWGIPLHPNTTFHGSFYAKEDSPDVGPVTA
                     SLVANQTGKVLASDTISGIGTDWKQYTFTLKTGAIQSSKENHLVLSVAHPGSVWFQLV
                     TLFPPTYHDRPNGTRSDLMEKLAAMHPQFLRFPGGNYLEGDHLNEWYDWKKTIGPLVD
                     RPGHPSPWRYWSTDQFGLLEFFEWCEDLHMDPLLAVYAGYSLAQEHVKPGPDLEPYVQ
                     AALDEIEYATGSTDTKWGAERAKDGHPEPFHITYVEIGNEDQFDRSGSYGERYAQFYK
                     AIKAKYPDLQLIATAPIKTMTPDVQDDHYYVRATDFFHDATHYDKIDRNGPKIFVGEW
                     ATREGTPTPNMGAALGDAAWMTGLERNSDIVVMASYAPLLVNVSPGAGAMQWDTDLIG
                     YDALRSYGSPSYYAQVMFG"
BASE COUNT         2244 a         2951 c         3299 g         2149 t
ORIGIN      
        1 caccaatcag ggccacaagg aagccaatca tgagcgcgcc aaagttcgca cccggcgcca
       61 tctcgttgta ttgctcggta atcgcgaata tgccaaaaat caggaatgcc gtgagaacga
      121 tttcgtcgcc aaacgcgtgg aggggggtaa ttcccggcgc cggatgagta aagaagacac
      181 cggcggcgcc tccagcggct cgcgtgagat ggtgcgttag gttgaattca ttgatgaccg
      241 gagagaatag ctgtaacacc aacgcagctc cggcgaatcc tccaagaatc tgggcagcgc
      301 aataggggac gacctgcttc caggagaatt tgcgataaag ggccagagcc agagtcaccg
      361 ccggattggc atggcaccca ctgatcgatc ccgtggtgta gatggctacc gcgacagcca
      421 gcccccacgc gatgcagacg ccccagtaag cgtgctggta agggctcgga tcgtaaaggg
      481 aatacattgc agcaaccgaa tcgccgaacg cgatgatgat gaacatggca actgcttcgg
      541 aaagaagcgc gcccgtaaat ttccgggtca tgaagtcctc gctggattgc agggaccatg
      601 gtattacgta cttgcctcac gttgagcgga ctgtgcgttc agtatttccc cacacgggca
      661 gcggccttga gcggcacgat cagggctgac tgggcgacga tgcggctgcg cttccggacg
      721 gcgcttcgct ctgcgagtcc tgcgcgtgac tggcctgaag cttttgccgc aaactctgat
      781 cggcggcctg agtgagtctc ttagtctttg cgaattcggc ctgggcctcg acgcgcttgc
      841 ccatggactg atagagccgc gcgagcctcc agtgcacctg cacgtcgtct ggcgcgattt
      901 ttgcagcagt ttgaaaatcg cggaatgccg catcgttatt gcccgcctgc gcgtcgagaa
      961 tgcccagatc gagccagggg agttcctgcc gggaattgag cttcagggct ttttctacca
     1021 ggggacgggc cacgtcggct cgattcagtc gaacgttgga atcagcccag tacgcgatgc
     1081 tctctgcgtc attgggctgg ttttcaacct caagcccgaa ctgctcggcc gcctcctcat
     1141 actggctctg cgcccacagc agatagccaa gtccgaaatg cacgcctggg atcttcggat
     1201 cagctttgac ggcattgcgg aactgctgga tcgcgctctc gtgatcccgc atctcgtcat
     1261 aggcttcgcc ggccagcatg tccgcctcgg cagactctgc attcagagcc agaatctcgt
     1321 gataaacgtc gagcacgcac ttatatttcc tggcccacaa acagctgtgc gccagcacca
     1381 ggcggtatgg gaggttttgt ggatcgcgtg tcgttgcatc gcggagatag ggaatagctg
     1441 cggcgtagtc gcccgcaccg taatccgcca tgccgatcag caggcgcagc cgctgcccgt
     1501 ccggcgaatc gggggatgtg cggtggtaga gcgaaacgaa tacgcgagca gatcctttca
     1561 tgtcaccgga cttgaacaga gcgagtccga gattcatctg caggccaggc atggacggct
     1621 tgatggccag ggccttccga tagtaacgga ccgcgtcgcc gtagtgcttt tgctgcgatg
     1681 aaatgacgcc caggttcgcg catgcatcgg catcggccgg atggctcttc agaattgtct
     1741 gccatgcgac ttcggcctga gccaactcac cttgctgctg cagcgtgatc gctgcgtccc
     1801 gttgctgagc ataagaaacc gtcgtctggg cggagccgcg caggcaagcc gccgcgacga
     1861 ccgcagcaac caaaactcgc aataatggaa ccctgttcaa gcactgctcc gacagccatt
     1921 gtcgcgcccg ggaataccgg ttcgcaatgg cggagaaaaa gaaagggcgc ggcagcagat
     1981 tcgccgccgc gcctcaggga ggcaaacaag ctgattagaa gatgaacttg ccgcccagtt
     2041 ccagagcacg cggctgatag attccggtcg tctggccgaa gtttccgtcc ttgttgttga
     2101 ggtcaaggtt ctggaactgg gtgtggttga aggtgttgaa cgactcgaac cgcagttcga
     2161 tccgcgggcc gccctctttg aacggaatcg atttgaagag cgaaaggttc cagttctcga
     2221 ggcccggcag ggtaacggcg tcccgcccag cgttgccgaa gccggcattc acgccggtgg
     2281 gtccgccgtt ccacggcgct accggatcgg tgaacttgct cttgtcgaac caggccgttt
     2341 gtttcttcgg ataggacatc tttccggtct ggttcggccg gttcgtggtg ccgccgccaa
     2401 gaccaacggt gtcacccccg ctgtagcgaa cgacttgagg aataccgctt tcgaacagcg
     2461 cgattccaga aaccgtccag ccacccacca cttcgcgagc gaagaggttg tcgctgtgcc
     2521 gggcgaacgg cagtccgtag acgaagctgg cgttgaagat ctgccggcga tcgaggccgc
     2581 ccgaggcgcg gtcgtagtta gtgttatagg ggttggacag accattgagg tcgttggtgg
     2641 ccgtgtcgat ctcgtgcgac caggtgtatg ccagctgcga ggtgagaccg tgcttgttct
     2701 cgaagcggat acccgtctgc agagcgttgt agtggaagtt ggtctcgttt tcttcctggt
     2761 tgatgttggc gaaaccaggg aagttgcgat aggggttggc gggcgcgcca ccggcgactg
     2821 cctggcggtg cgtgatgtct ccaacaggca gagtgttgat ctgccggtcg ttgttctgat
     2881 cccagccatt ggacccgacg tattgcacga cggccacgac ggaaggagcg acctcacgct
     2941 ggatgccgag actgtagttc gaagttcccg gcggcggata gtgatacttg atgttggtca
     3001 ggttcgatgg gaagtactgc tgtgcagccg tctgcccgtt cagagcgctg gtgtgcggat
     3061 cggagaagta cacgctgtcg gccgatggct gataggcgaa cggcgggttg agggcggcgt
     3121 tatacacatc gttgccctga acgcgctcga agaacacgcc gtagccgccg cgaagaaccg
     3181 tcttgccgtc tccgcggatg ttataggcaa agccaacacg aggctgccag gtaaagtgga
     3241 agttctccac gttgccgcgt gggaagccac ccacaccggc ttcgcgaatg ccgttgagat
     3301 agaacagttc gttgcccgtc ttgctgaagg tggagagctg cgaggtgatg agccggccgt
     3361 tcgcgtcgac cggattgccc ttcgaggtat cgtaatcggc gataacgaag ttcgagaacg
     3421 catcgtaccg ttcgaaggtg tgcggcaggt catcgtaacg gatccccagg ttaagggtga
     3481 ggttacgatt cacatgccag ttatccatgc catagccact ataggtgttc gtgacccagt
     3541 gctttccggc gaggtattcg agctggctga agttggccgt gtctcccagc aaaaagttga
     3601 cataggagtc accggagaac gcactcgtgt tgaagatggc cgttccgttg gtattggcct
     3661 gcagctcctg gttcttgtaa gcgtgcagcc agctgaaccc gaacttgaac tggtggcgac
     3721 ccttcgtcca cgagagatcg tctttgtact ggaagccctc gtacccgttc ttccagggga
     3781 agtagctcgg gctccaggtg gtattgatgg gagtgccctg cagctggatt tcgggcatgc
     3841 gcgccatacg gttattcgcc gttgggaaaa ggctgaccgc actccatccg ctgggctgga
     3901 cgaagctgct gccgccactc ttgccggcaa tcggagtgag cccgatctta ttgccggcat
     3961 agaagaaccc ggtctcgttg agcagtgaag gagaataggt ctgcgtgaga cgaatgacgg
     4021 cggtgtacga ggggttggtc atggccgtgc cgaccgtcgg gtaggtgctg ttgccccaaa
     4081 gcggtgggta gaactgctta gccatggcgt catgcagata gtgacccatg atctggtact
     4141 tggaactgaa ggtgtggtcg atgcgaacca cgtcttcgcg aatgttatct ggctgcggga
     4201 tcgaagcggt gtactgcgag gtgccgaggt tgggcttggg aaatgttccc gaattcagct
     4261 cgagaacagc gttcggatcc ataaggttag cgggaatgac attacccgga aaccgctcac
     4321 cagcggtcaa cccatcctgt gcgtataagg cgagcttcgc aggatcggtg gttttcggca
     4381 cgcagggcgc ctgcacgccg gcattgcagg ccggactcgc cgtggtggga ttgttccacg
     4441 ggacgtacgt gaggttggtg ccagcggtcg ggaagttggc agcagggatg gtattcgcag
     4501 tcagcggcgt actgcccaga atcagacgcc gccattcctc gttccagaag aagaatgtgc
     4561 ggttgcgatt ctcgttgtag acgtgaggta tgaacagggg accgccgata ttgccgcctg
     4621 gctcgttgag ctggaagatg ggacggctgt tgccggtagc tctccgtttc acaaagtagt
     4681 cgttcgcgtt gtaagcggtg ttgcggttga actcatacag ttcgccatgg aaatcgtggc
     4741 cgccggattt gagcgccatc acgatcgttc ccccggaacc aatgccgtag tcaggcgggt
     4801 agttgctgtc gagggtctgg aactcggcaa tggcgtcctg agcgggcagg ttcatgaagc
     4861 agccgccgca gccgcgatcg ttttgctcgc cgccatcaat cagatagacg ttatgcgtgt
     4921 tgcgcgtgcc gttgaagctg atgctgttgg cagacgtcag cgcatcgacg ccgccgaagg
     4981 aaggcagcgt gctggagacg ccgaggccga gggccgcgag ctgcgtgacg ttacggccgt
     5041 tggtggagag ctgttcgact tgttcagaac tgataagggt gctgacttcg ctggtttcgg
     5101 tctgcgtttg aagagcattc gcctggacgg aaacggtttg tgtctgactt ccaactttga
     5161 agacgatgtc agctgcaacc gtctgcgcga cgttaacgac gatgccggtt ttggtgaagg
     5221 tctcaaagcc aggagccgta gccgtcatgg tgtaggtgcc gatggcaaga ttggcaaagc
     5281 gaaattcgcc cactgagttg gagacttgct tgcgctcgac cccggtggca gtgttggtca
     5341 gggtgattga cgcattggcg acgacggcgc cagtggagtc ggtgcagtcg ccgttaatgg
     5401 tggcattttc ctgggcgcac aacgtagagc tcagggcaaa gacgagcgcc agaacagcga
     5461 acgaaactga gaaaagccgg cgaatagcgt ggatgcgggt agagaaaggt gctgggcgag
     5521 caattgcaat catgggtgcc tccgtgagca aacgttttct gaaactggaa gaccggttct
     5581 tccaccggaa tttttttagg ggaacacagc ctgatttatt acaggcggta aacaattact
     5641 cccacgaacg aggcgttgtc aaggcatttc agggctgttc tgaatgccgt ttatggcatc
     5701 cctgaaaccg tttacccggc aaatcgattg actgcgccaa tatcccaatt ccggactggc
     5761 cggaaggtct ccccggactg aagcttaggg acgagcaggt caatccgacg ccgcccagcg
     5821 gatccttcca gaacctcatc aatgacggtc tgcggccagg tgttgggtgc ttcgctccaa
     5881 ccgtcttgcc acacggctgg cacctccttc cacacactct cggggggcaa gaatcaaaat
     5941 gacaccggtg atccgcctgc aacgaaagaa aagaattgtg ccactttgag tggttggata
     6001 aatcacttac aggtccgcct cctgccggat ccgccgcact gcggatcaaa aaagcgtttt
     6061 ctttaaattg accccgtgcg cccgaggttt tagggtggtg tgtcgtcgcc gcaggcaaat
     6121 ttcgcagcga atccatcagg cggcgcaatg cattcatcac gaggggacct tatgccgaaa
     6181 gccgggatgc gcatctgggc agcgtttgca atctgtctgg ggatgttggt cgtgaaagcg
     6241 ggagctcagg atgtttcgag gctgcctttc atgaattcga atctctcgcc cgaggagcgg
     6301 gccagggatc tggtcagcca catgacgctg gaggagaagg cgtcgcaact cgtcaatcag
     6361 gcacgcgcaa ttccgcgact aaatgtgccc gcctatgact ggtggagcga ggcgttgcac
     6421 ggggttgccg tgaacggcac cacggaattt cccgagccga tggggctggc ggcgaccttc
     6481 gacgccccgc gcattcatga gatggcgatc gacatcggca ttgagggccg cattaaacat
     6541 atgcaggctg agcgagccgg ccacagcaac atcatggagg gtctcgattt ctgggcgccg
     6601 aacctgaaca tctttcgcga tccgcgatgg ggacgcggcc aggaaaccta cggcgaggat
     6661 ccctacctca ccggccgcat gggcgtcgcc tttgtgaccg gcatgcaggg cgacgatccg
     6721 aaatactacc gcgtgattgc aacaccgaaa cactatgcgg tgcacagcgg accagagccg
     6781 acgcgacaca tggccgatgt cgacgtcagc aagcacgatg agatggacac ctatttgccg
     6841 gcgtttcgcg cggcggtcac cgagggcaag gccgattctg tgatgtgtgc ctacaacgga
     6901 atcaagggcc agccggcttg tgcgaatcaa ttccttctgg aagatcagct gcgcaaacac
     6961 tggggattca agggatacgt ggtgtcggac tgcggagccg tcatcgacat ctatgccggt
     7021 catcattacc ggccaacgca agcccaggcg tcgggaatct cgctgctgcg cggtatggac
     7081 aacgagtgcg cagacttctt cgcgaaggtg catgacgacc acgattacaa gccttatatc
     7141 gacgccgtgc gccagggcta cctgagcgaa aacgacgtgg acacggctct ggtgcggctg
     7201 ttcaccgcac gcatgaagct cggcatgttc gatccgccgg atatggtgcc ctacacgaag
     7261 atcgacgaaa aagaactgga cagtgcagaa catcgcgctc tggcgcggaa gctggcgaat
     7321 gaatccatgg tgctgctgaa gaatgacggc acgcttccgc tgaagtcgag cgtgacgaag
     7381 attgcggtca ttggtccgct ggccgatcag aatcgcgtgc tgatgggcaa ctactccggc
     7441 atgccaaccc attccgtgac ggtactggat ggactgaagg cggaatttcc aaacgcgaag
     7501 atccgttttg tcccggggac gcaattcctg cgcaacgatg gcgatcccct gccgaacagc
     7561 ttgctgacca ctccggatgg taagcccgga ttgaaggcgg agtacggatc aatcgagggc
     7621 atggacctca acccgagatc gaaaccaaag ccgctaacca ctagaacgga acccaacgtg
     7681 aatctcgcag ccggcagcct tcctgccgag gcccagggca agcccgcgat ttccgtggag
     7741 tggtccggat ttctgacagc cccggacacc ggggattacc tgctggggat aacggtcgat
     7801 ggttttggcc ggttgatggt ggacggcaaa cccttcgctc aggttttcag catgggcggc
     7861 agaaatgcca agatgggccg cattcatctt gaacgcggcg aaaaagtgcc gatcacggtg
     7921 acctatgcaa agctgaatcc cgccggcgag ccaaaggcgc aactgctgtg gggacgatac
     7981 aaccccgctc cttctcccga ggctgtcgca gcggcgaagg aagccgacgt gaccatcgcg
     8041 gtcgtcggca ttaccagcga actggaaggc gaggaaatgc cggtaagtga gcccgggttc
     8101 cttggcggcg atcgaaccag cctcgatttg cctcagccgg aagagaacct cattgaaacc
     8161 gtcgctgcca ccggaaagcc gctcgtcgta gttctgatga atggcagcgc gctcagcgtg
     8221 aactgggcca aagcacacgc taatgcgatc ctcgaggcgt ggtattccgg ggaagaaggc
     8281 ggcgcggcca tcgccgaaac cttgagtgga aaaaacaacc ccgccggacg acttccggtg
     8341 acgttttata ccggtgtcga ccagctgccg aacttcgaag actactcgat gaacaaccgg
     8401 acttaccgct atttcaaggg cgagccgctc tacccgtttg gatatggcct tagctataca
     8461 aaattcagct acagcgggct ggaagtgcct gccgaagagg tgaatgcggg cgatccggtt
     8521 catgcaactg tcaccgtcac aaacacaggc agcctcgccg gggatgaggt tgtggagctg
     8581 tacctgcagt tccctgatgt tcccggtgcg ccgctccgcg cccttcgcgg atttcagcgt
     8641 gtacacctcg aaccaggcgc aagccagaag gtggacttca ctctgggccg tcgcgatctg
     8701 agcatggtaa cggaactcgg tgatccgatc atcgccgacg gagcttacac gatcaccatc
     8761 ggtgggggcc agcccaacac cgaggcgcct gtcgccacgg gtcactttcg ggtcaagggc
     8821 cagctgatgt tgccagagtg aagagcaata aacaggctgc gcctaaccag cgcagcctgt
     8881 tcgatcggag gggctgtgtc cctccgggcg cagccctcgg tagcgtctaa gccgtgcggt
     8941 gtgtcgccga attgctcgat cggcagaaga atcggccaac gaccaggaaa acgcggcgta
     9001 gacaggcaat tgctgtggta tacgtttctc gtcgtagccg ctgtcgcagg agtgagtgct
     9061 tcaatgagaa tgcagcgttt cgcaatccta tccatcgtcg cgatgttcgt tttcactggt
     9121 ccggtatttc ttggacagtc cgctggtccg gcggtcatca ccatccacac cgatcaaaca
     9181 gcatcaaagg tcagtccgac tctctacggg ctcatgaccg aggagatcaa tcactcttac
     9241 gagggtggtc tgtatgcaga gctgatccag aacgatacct tccgctccga ctggtccggc
     9301 gttaacgaat ggattctggt ccagaatggc aatgcgtccg cgaagatggg aatcgacaag
     9361 accaccggtc ccagtgaggc tctgccgtat agcctgcacc tggaggtgag cgaagccagc
     9421 gcctctggat ccggcggtat tctgaacggc gggttctggg gaattccgct gcatcctaat
     9481 accacttttc acggctcgtt ctacgcgaaa gaagactctc ctgacgtggg acctgtgacg
     9541 gcgagcctcg tcgcgaatca gacgggcaag gtgctggcga gcgacacgat ttctggtatt
     9601 ggcaccgact ggaagcagta caccttcaca ctgaagaccg gtgctatcca gtcctcgaaa
     9661 gagaatcacc tggtcctgtc cgtggcgcat cccggcagcg tgtggtttca gctggtgacg
     9721 ctgtttccgc ccacctacca cgatcgccct aacggaaccc gttcagacct gatggagaag
     9781 ctggcagcaa tgcacccgca gttcctgcgc ttccctggcg gaaactacct cgagggcgat
     9841 cacctgaacg agtggtacga ctggaaaaag accatcggac cgctcgtcga tcgcccaggg
     9901 cacccgagcc cgtggcgata ctggtccacc gatcagttcg gactgctcga gttttttgaa
     9961 tggtgcgaag acctgcacat ggatcccctg cttgcggtct atgccggcta ttcgctggcc
    10021 caggagcatg tcaaaccggg gccggatctc gagccgtacg tccaggcggc gctcgacgaa
    10081 atcgaatacg ccaccggcag caccgataca aaatggggtg ccgagcgcgc aaaggatggt
    10141 caccccgagc cgttccacat cacctatgtc gagatcggca acgaagacca gttcgaccgc
    10201 tcaggcagct acggtgagcg ctatgcgcag ttctacaagg cgatcaaggc gaaatacccg
    10261 gatctacagc tgatcgcgac cgccccgatt aagacgatga cgcctgatgt gcaggacgat
    10321 cactattatg tgcgcgctac cgattttttc cacgatgcga cgcactacga caagatcgat
    10381 cgcaacggtc caaagatttt tgtcggcgag tgggcgacac gggaagggac gcccacacca
    10441 aacatgggcg cggcacttgg cgatgcggca tggatgacgg gtctcgagcg caacagcgat
    10501 atcgtggtga tggccagcta tgcgccgctc cttgtgaatg tcagcccggg agcgggagcg
    10561 atgcagtggg ataccgatct gatcggctac gacgcactgc gcagctacgg ttcgcccagc
    10621 tattacgcgc aagtcatgtt cgg
//
LOCUS       DASZYN010000727         5923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1139293, whole genome shotgun sequence.
ACCESSION   DASZYN010000727 DASZYN010000000
VERSION     DASZYN010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139293"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..2842)
                     /locus_tag="VHE33_18660"
     CDS             complement(233..2842)
                     /locus_tag="VHE33_18660"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HVT99528.1"
                     /translation="MHLGPGTILGDYEILSPLGAGGMGEVYRARDSRLGREVAVKVLP
                     VSFVRDSERLRRFEQEARAAAALNHPNILVVYQMGMQEDVPYLVSELLEGETLRARLS
                     RGPLPLRVAIDSGVQIARGLAAAHRKGIVHRDLKPDNLFLTKDGHVKILDFGLARVVP
                     HEGESSAPTLGGQTAPGVLMGTVGYMSPEQVRGQTADQRSDIFSLSVVLCEMLTGRST
                     FEKPTSAETMAAILNENPPVLAELSATVPPGLQRVVQRGLEKDPDRRFQSAADLAFAL
                     EALSDGSLTALAEGHAGTGAAKAHPRRRGAAILAGVVVLAILAALGWMELRTPEPPSM
                     TNYAQLTHDGVQKSLIGSDGARLYISAITSAVQEIAAVPVAGGELAKTEMPQPGMIPF
                     HLSADGSQFLAIDARGVPFRGPLWSVPVLGGSPRRLGETIGNDAAWSPDGRLLAYADQ
                     GDLYVGQADGSGAKKLLTVDGLISHLVWTPDSGSVRFDTSQGFGTGIGQHLLWEVAAD
                     GSKLHRLLAGWHTPPDECCGVWSADGRYFLFVSQGQIWALPPGRFLHPEGKPIAVTSS
                     PMSLSSPLASADGKKLFLVGATFRGELTRVDTRNGAFEPYLGGISAEYVSFSKDGQWI
                     AYVAYPEGTLWRCRADGSDKAQLTFPPLRAVLPQWSPDGRSLVFFEFPVSSSQPARIY
                     QVPAEGGTPVQLIPDDPLNQQDPNWSPDGTRIIFAGSAGDAADHPATPAIRTWDAATH
                     RVSAIAGSQRMFSPRWSPDGAKLVALSDDSRTLMLLDMKTGQWSRLAQGTFGWVNWSK
                     DGQSIYVLDFTGKGGVLRIHLDDPKVESVVDLKDFVTTGQFGGWLALTPDDSPLLLRD
                     RGTQDVYSLDWKD"
     gene            complement(2893..5499)
                     /locus_tag="VHE33_18665"
     CDS             complement(2893..5499)
                     /locus_tag="VHE33_18665"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HVT99529.1"
                     /translation="MPFGPGSHLGEYEIVAPLGAGGMGEVYRARDTRLGREVAIKVLP
                     DLVSSDPERLRRFEQEARAAAALNHPNILVVYQMGSLEGLPYLVSELLEGETLRERLD
                     RGALGLRQVIDYGVQIAHGLGAAHAKGIVHRDLKPENLFLTRDGHVKILDFGLARVVP
                     LPGDKTTLPPGAVDSTPGVVLGTVGYMSPEQVRGEPADARSDIFSFTVILREMLTGKR
                     TFEKTSSAETMAAILHDDPPPLSAEPSIPAGLQRVLQRGLEKSPERRFHSASDLAFAL
                     EALSDPALATPLPRDVPQTGEPRRLPRWVAGAAFAGVAIVGLALWFWVREAKPPAITN
                     YVQLTTDGEQKRLLGTDGARIYLTKLGPSVENVDAIQIAGGAQSNIAMPALDIGPVNI
                     SADGAEFLVISGGGVPVRGPIWSLSVLGGSPRRLGQAKGNYAAWSADRQWLAYADAAD
                     LYVARGDGSDAHRILNVGTLITHVVWSPDNRRLRFEASEGLGPFDGLQRLWEVNADGS
                     NPHRLLEGWHNPPDECCGVWSRSGKYYLFASRGQIWALARPNWLHPHPRPVQLTSSPM
                     ALSWPLPGLDDRKLFVVGATYHGELMRYDLKSKEATPFLGGISAQYVSFSQDGKQLAW
                     VSFPEGTLWRSGVDGSNRVQLTFPPMQVVLPRWSPDGSRLVFFKEPIGSGERARMYEI
                     SAEGGSPAELIPGDDGNQQDPNWSPDGKQIVFARGATEATADPGQPQVRIFDRATRTV
                     QPVPGSGGLFSPRWSPDGRTLAALGADSKSLSTFDFATQKWTTVARGTFGWLNWSRDG
                     RSIYLLDFSGRGAVLRLRLADGKMETVVDLRNFAIAGGFGGSLTLTPDDSPLLLRDRG
                     TQDVYAMDWGAS"
BASE COUNT         1137 a         2164 c         1614 g         1008 t
ORIGIN      
        1 agaacccatg cgcccgattg actgttcaga ccctgccccc aggcaaagcc gccgagcgcc
       61 agcagcattc ccaggcagct tgcacgcctc agcatggcgg catgctaccg gcccagggca
      121 cccggaaaac atcccacgga aaggtcggga ggacgccaga gcgttactgc cacttcagcg
      181 tgatctccca ggttgcccgg ctggactcga cccaatcaaa aacccgcctg cgtcaatcct
      241 tccagtccag cgagtacacg tcctgcgtcc cgcgatcgcg cagcagcaag ggggaatcgt
      301 ccggcgtcag cgccagccac ccgccaaact ggcccgtcgt gacgaaatcc ttcaggtcga
      361 ccacgctttc cactttgggg tcgtccaggt ggatccgcag cacgccccct ttgcccgtga
      421 aatcaagaac gtagatcgac tgcccgtcct tcgaccagtt cacccagccg aaggtcccct
      481 gggccaaccg gctccactgg ccggttttca tatcgagcag catcagcgtc cgtgaatcat
      541 cgctgagagc caccagcttc gctccgtccg gcgaccatcg cggcgaaaac atccgctgcg
      601 aaccggcaat cgcagagaca cggtgcgtcg cggcgtccca ggtgcgaatc gccggcgtgg
      661 ccgggtggtc cgccgcatcc cccgccgacc ccgcaaagat aatgcgcgtc ccgtcagggg
      721 accagttcgg gtcctgctgg ttcagaggat cgtccggaat cagctgcacc ggcgtccctc
      781 cttccgcagg aacctgatag atgcgggccg gctgactcga actcaccgga aactcgaaga
      841 agaccagcga tcgcccatcc ggcgaccatt gcggcagcac ggcgcgcagc gggggaaacg
      901 tgagctgagc cttatcgctc ccatcggccc ggcagcgcca cagcgtgccc tccggataag
      961 caacatacgc gatccactgg ccgtctttcg aaaacgatac gtactcggcc gagatgcccc
     1021 caaggtacgg ctcgaatgca ccgttgcgcg tgtcaacccg cgtcagctcg ccgcgaaagg
     1081 tcgcccccac cagaaaaagc ttcttcccat cggcgctcgc caagggcgac gacaacgaca
     1141 tcgggctcga cgttacggct atcggttttc cctcggggtg caggaaacgg ccgggcggca
     1201 gcgcccagat ttgtccctgc gacacgaaca ggaaatagcg cccgtccgcg ctccacacac
     1261 cgcagcactc atccggcggc gtatgccagc ccgccagcaa acgatgcagc ttcgacccgt
     1321 ccgccgccac ctcccacaga agatgctgcc cgattcccgt cccaaatccc tgcgacgtat
     1381 cgaaccgcac gctgccgctg tccggcgtcc acaccaaatg gctgatcagc ccatccaccg
     1441 tcagcagttt ctttgcgccg gagccgtcgg cctgccccac atacaaatcg ccctgatcgg
     1501 catacgccag cagccttccg tccggcgacc atgccgcatc gttgccaatc gtttcgccca
     1561 gccggcgcgg cgatccgccc agcaccggca cgctccacaa cggcccgcga aacggcacgc
     1621 cgcgcgcatc gatcgccaga aactgcgatc catccgccga caggtgaaat ggaatcatcc
     1681 ccggttgcgg catttcagtt ttggccagct cgccgcccgc caccggcacc gctgcgatct
     1741 cctgcaccgc cgacgtgatc gcgctgatgt agagccgcgc cccgtcgctc ccgatcagcg
     1801 atttctgcac gccgtcgtgc gtcagctgcg cgtagttggt catcgagggc ggttccggag
     1861 tccgcaactc catccagccc agcgctgcga ggatcgccag cacgaccact cccgcaagaa
     1921 ttgccgcccc gcggcggcgc ggatgcgctt tcgctgctcc cgttccggca tgcccctccg
     1981 caagcgccgt cagcgaccca tccgacagcg cttccagcgc gaatgccaga tcggcggcgg
     2041 actgaaaccg tcggtccgga tctttctcca gcccgcgctg cacgaccctt tgcagtcccg
     2101 gaggaaccgt ggccgacaat tctgccagca ccggcggatt ttcattcagg atcgccgcca
     2161 tcgtctccgc tgaggtcggc ttctcgaatg tcgaacgccc cgtgagcatc tcgcacagca
     2221 ccacgctaag cgaaaaaatg tcggagcgtt ggtccgccgt ctgtcctcgc acctgctccg
     2281 gagacatgta ccccaccgtc cccatcagta cgccgggcgc ggtctgcccg cccaaagtcg
     2341 gcgcggagct ctctccctcg tgaggcacca cccgcgccag cccgaaatcc agaatcttca
     2401 catgcccgtc tttggtcagg aagagattgt ccggcttcag atcgcggtgc acgatccctt
     2461 tcctgtgtgc cgccgccaaa ccgcgcgcga tctgcacgcc gctgtcgatc gcaacccgca
     2521 gcggcagcgg gccccggctc agccgtgcgc gcagcgtctc tccctccagc agctccgaaa
     2581 ccagatacgg cacatcctcc tgcatgccca tctggtaaac caccaggatg ttcggatgat
     2641 tcagcgcggc agccgcgcgc gcctcctgtt caaaacggcg cagtcgttcc gaatcgcgca
     2701 cgaaggaaac aggcagaacc ttgaccgcca cctcgcgtcc cagccgcgaa tcgcgcgcgc
     2761 gatacacctc tcccatcccg ccggcgccca gcggggacag aatttcgtaa tcgccaagaa
     2821 tcgtgcctgg ccccaggtgc attgcccggg attataggcg taagcggctt cccggcgcag
     2881 acaatcttgg ctctacgacg cgccccagtc catcgcgtac acatcctggg tgccgcggtc
     2941 gcgcagcagc agcggcgaat cgtccggcgt cagcgtcagc gatcccccaa atcctcccgc
     3001 aatcgcgaaa tttctcagat ccaccaccgt ctccatcttt ccatcggcaa gccgcaagcg
     3061 aaggaccgcg cctcgcccgg aaaaatccag caggtagatc gagcgtccat cccgcgacca
     3121 gttcaaccat ccgaacgtcc cgcgcgccac tgtcgtccac ttctgcgtgg caaaatcgaa
     3181 cgtcgacaag cttttcgagt cggctcccag cgccgccagc gttctcccgt ccggcgacca
     3241 gcgcggcgag aacaggccgc ccgaacccgg aaccggctgg acggttcgcg tcgcccgatc
     3301 aaaaattctc acctgcggct gccccggatc ggctgtcgct tcggtggctc cacgcgcaaa
     3361 cacaatctgc ttgccatccg gagaccagtt cggatcctgc tgattgccat catcgccggg
     3421 aatcagttct gccgggcttc cgccttcagc tgagatctcg tacatccgcg cccgctctcc
     3481 ggagccgatc ggctctttga agaacaccag tcgcgaaccg tccggactcc atcgcgggag
     3541 caccacctgc atcgggggaa acgtcagctg cacgcggttg ctgccatcca caccgctccg
     3601 ccacaacgtt ccttccggaa aagaaaccca ggccagctgc ttgccgtctt gcgaaaagct
     3661 gacatattgg gccgagattc caccgagaaa cggtgtcgcc tccttcgact tgaggtcata
     3721 ccgcatcagt tcgccgtgat aggtggcgcc tacgacgaac agttttcggt cgtccaggcc
     3781 tggtaagggc cacgatagtg ccatcgggct cgatgtcagc tgcaccggcc gtgggtgtgg
     3841 atgcagccag ttcggccgcg ccagcgccca gatctgaccc cttgaggcaa acaaatagta
     3901 ttttccgctc cgcgaccata ctccgcagca ctcgtccggc gggttgtgcc atccctccag
     3961 cagtcggtgc gggttggatc cgtccgcatt gacttcccac agcctttgca gcccatcaaa
     4021 cgggcccagc ccctccgagg cttcaaagcg gagacggcga ttgtcgggag accacaccac
     4081 atgcgtgatg agcgtcccca cattgagaat cctgtgcgcg tccgagccat cgccgcgcgc
     4141 cacatacaaa tcggcggcat cggcgtacgc cagccactgc ctgtccgccg accacgctgc
     4201 gtaattcccc ttcgcctggc cgagcctgcg cggtgaaccg cccagcacgg ataagctcca
     4261 gatcggcccc cgcaccggaa ctccgccccc gctgatcacc aggaactccg cgccgtccgc
     4321 ggaaatattc acggggccga tgtcgagagc cggcattgcg atgttgctct gcgcgcctcc
     4381 tgcgatttga atcgcatcca cattctcgac agacggaccc aatttcgtca gatagatccg
     4441 ggcgccgtcc gtccccagca gcctcttctg ctcgccatcg gtggtgagct gcacgtagtt
     4501 cgtaatcgcc ggcggcttcg cctctcgcac ccagaaccaa agcgccaggc ccacgatcgc
     4561 cactccggcg aaagccgcac cagccaccca ccgcggcaag cggcgtggct cgcccgtctg
     4621 gggaacatcc cggggaagag gcgtagccaa tgcaggatcg gacaatgctt ccaaagcaaa
     4681 cgccagatcc gacgccgaat ggaaccgccg ctccgggctc ttctccagtc cgcgttgcag
     4741 cacgcgctgc agacccgcag ggatcgaggg ctccgctgaa agtggtggag gatcgtcatg
     4801 cagaatcgcc gccatggttt ctgccgagct tgttttctcg aacgtccgct tgcccgtgag
     4861 catttctctc agaatcacgg tgaacgagaa aatgtccgag cgcgcatccg ccggttcccc
     4921 tcgcacctgc tctggcgaca tgtaccccac cgtccccagc accacgcctg gtgtcgaatc
     4981 caccgcgcct ggcggaagag tcgttttgtc cccaggaagc ggtaccaccc gcgccagacc
     5041 aaagtcgagg atcttcacgt gcccgtcgcg ggtcaggaac aagttctccg gcttcagatc
     5101 ccggtggacg attcccttcg catgcgccgc tccaagacca tgcgcgatct gcacgccgta
     5161 gtcgatcacc tgccgcagac ccagcgctcc gcgatccagc cgctcccgca gcgtctcgcc
     5221 ttccagcagc tccgaaacca gatacggcag gccctccagc gagcccatct ggtacacgac
     5281 gagaatattc gggtggttca gcgccgccgc tgcacgcgcc tcctgctcaa aacgccgcag
     5341 tcgctccgga tcggaggaga ccaggtcggg cagcactttg atggcgacct cgcgccccag
     5401 tctcgtatcg cgcgcacgat acacctcgcc catcccgccg gcacccagcg gcgctacgat
     5461 ttcatattca ccaagatggg acccgggacc aaacggcatt tgcccaggat tataggcgaa
     5521 ccaggagagc gcgaagggct tttcgccagg agcgtttagc gaagagcgtt tagccagaag
     5581 cttttcgcga gaagcttttc gccagaagcg tttagcgaag agccttttag ccagaagctt
     5641 ttcgccagga gcgtttagcg aagagcgttt agccagaagc ttttcgccag gagcttttcg
     5701 ccagaagctt ttcgccagaa gcttttcgcg aaccgctctt tagctaaccg ctccaccagc
     5761 gaaccgcctc accagttgcc ttctcacacc ggcggctcac cctccaccac atgcaccccc
     5821 ggcggcggca caaactggaa cgccgcatcc gccgcaggca cattccccgc ctccccgctg
     5881 aactcgaaac ggttcgtcac tccatcggtc tcttcaatcg tca
//
LOCUS       DASZYN010000728        50865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_683889, whole genome shotgun sequence.
ACCESSION   DASZYN010000728 DASZYN010000000
VERSION     DASZYN010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 50865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50865
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683889"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="VHE33_18670"
     CDS             <1..137
                     /locus_tag="VHE33_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HVT99530.1"
                     /translation="LEAAIRAYVDINNEKPRPFVWTKTADEILDSIKRYCLLTSETGH
                     "
     gene            complement(323..1225)
                     /locus_tag="VHE33_18675"
     CDS             complement(323..1225)
                     /locus_tag="VHE33_18675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99531.1"
                     /translation="MITSASALVGCFLALLASACTSVRTDEGLVILDVSLAAGLASPA
                     AVRFSVPERPSVPSHEVPYDPLRPLHFGYYLPGPAGATQVQAEALTASGCVVGQGTAI
                     VDVALGRVSPAVPLVLAPATGTRPDGGAICRSVGDGAADQRRLDAAATHDARDGSGPT
                     DAKSTTDAKRTADAHADSHPVDTSAPACLPATRVCTASSTCCSGLTCSSNSLGKVCCG
                     AYGARCTLANGTDCCGELECINGGCCLPATRPCQGGDCCLGLTCGTTSLGKVCCGSAG
                     APCTRTDGADCCGTLSCRNGTCAP"
     gene            complement(1222..1800)
                     /locus_tag="VHE33_18680"
     CDS             complement(1222..1800)
                     /locus_tag="VHE33_18680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT99532.1"
                     /translation="MIAGVTLVVMAFAVGASPSAAPAAPADLRARDLFQQQRYADALA
                     IYTDLYARTHHPTYLRNAGRCHQMLRQPEQAIAYFQTYLHDAHDLPAAERSEVEGYIA
                     DMQALQRARVQMLAPLPPESAQSETGGRVTSPASAVLVEAPPSRATPVTHRWWFWTAV
                     GAVVVGGVVTAAALASHGNSRLPCPDGAVCPP"
     gene            complement(1809..3311)
                     /locus_tag="VHE33_18685"
     CDS             complement(1809..3311)
                     /locus_tag="VHE33_18685"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HVT99533.1"
                     /translation="MIGERVGNFRITGLLGGGGMGTVYLAEHPGLGRRAAIKVLHAQL
                     AADPEIVQRFFNEAHAANAVGHRGIVEVLDFGTLASGAPYIVMELLPGESLAGRLRRA
                     GRLPIAESVDIADQVADAVGAAHRKGIVHRDLKPDNLFLTLDPERPDRTAVKVLDFGV
                     AKLQQPPDGAGDLRTRTGIVMGTPQYMSPEQCRDARDVDHRADIYSLGVVLHEMLAGA
                     PPFASTSWGELVHLHIGVAPPPLRACGVDVPVLLEAIVLQALAKDPAARFASVDDLRA
                     ALRAIGPGAPVRTLVMSEGGREISTPVAAVAPFATAVMPESRPRQRSTTLSETASEVE
                     GRPAAGGRSHWVVAAAAAATLLAVLAVGVALRAPRRGLPVTSSATVSAPVTAPPDPLP
                     ITVEIVTEPVGASVVRVRDGQRLGTTPLQLSWPGATGVEPLDVQKEGYRSERVVVPLD
                     RGITARLHLESLAAPAAAVAPSGGVETPLRRRPAPTPGHKKHLKPEPLKI"
     gene            3646..4314
                     /locus_tag="VHE33_18690"
     CDS             3646..4314
                     /locus_tag="VHE33_18690"
                     /inference="COORDINATES: protein motif:HMM:NF024001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3761 domain-containing protein"
                     /protein_id="HVT99534.1"
                     /translation="MRQNVVVAKARRATKPRTERRGSQLRETDLARKMPAMNTRVLFL
                     VVVLMLTFVPASFAQAPAVAPTTPATVICKDGTSSKGGRGACSGHGGIDHGAPSSIGH
                     TPSAGADPKAGPAESAGAGDVHCKDGTASKGGRGACSGHGGIDHGAAASVPSSRPSPT
                     PPPLSIPTAGPSRNAPSRPTTPANDNGTPTATCKDGTTSFAKHHQGACSHHGGVAQWL
                     DGTN"
     gene            complement(4367..4951)
                     /locus_tag="VHE33_18695"
     CDS             complement(4367..4951)
                     /locus_tag="VHE33_18695"
                     /inference="COORDINATES: protein motif:HMM:NF012253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HVT99535.1"
                     /translation="MRAIADGEGGVVSRLSKAHPELAKSALPVGATRQTTRQYLLTAI
                     DHYVYAGDTALHVAAASDGAKIVRELLRLGAAVGAANRRQAVPLQYAVDGGPGAPGWR
                     PEAQAETVTLLLEPDADPNVADAGGTTPLLRSVRNRCAAAVRALLDGGADAERQNKHG
                     SRPRDLAVVTSGRGGSGSAEARAQQREILRLLGS"
     gene            complement(5402..9130)
                     /locus_tag="VHE33_18700"
     CDS             complement(5402..9130)
                     /locus_tag="VHE33_18700"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVT99536.1"
                     /translation="MLRRVAETPRALAARRVIEPGATLGDRFIVERKLGAGGMGQVFA
                     VFDPERQTRVAAKVLGWLSAESIAQIKHEFRVAEAIVHENLVRLHELFSDRDLWFFTM
                     DLVDGVMLPVLLGREPSRRRAPGFLREIFRQLAVALNALHATSMIHRDLKPSNFLLTR
                     AENRVVLLDFGLARPPDLGERGDRAGTPMYMAPEQALGEAPTEAADWYSFGVVLHEAL
                     TGQLPVARRTPGAPMDAPDGLGELCGRLLALDPNDRPSGTEVLQALGHERRERGRRST
                     NELKTGLIGREDELAALQSALARTRAGVPAVVRVTGTSGIGKSVLLQRFAEIARGQGA
                     LVLRGRCRERESIAFKAVDGLVDCLIRLFESLPEQEVVQLLPPSIGDLTVVFPALGAV
                     PAVARLKHPQLVADRGIRRQLAVAAFVELIARLRRRAPLVICLDDAQWSDRESALLLA
                     PLLRVTDAGPLLLVISERGGANNPGPLVEALYGDGSPSLPTLENVALGPVSEASGEQI
                     ALAMLDVPSVDRVGVARRISREARGHPLFIAELAHTAGLRDQLDRPSPSTLTDLLAAR
                     MEPLSSMARRFLQVVSVAGGPVARGLVRRATGVSLADAHQVVDLLRSKHLLRSDGLTD
                     ADAVDTHHDRVREIVLRRLTDGERRLSHLHLARTLEADPGTALNQLAAHFEAAGELEK
                     AGSYWLAAADAAMRSLAFAKAADLYQKALEHAQLSIDDRRIVLRKQAEALVHAGRGVA
                     AAEVFLDLASHAPADESLELRRAAAEQLLLAGHFERGMSAIDEVLTAVGMSRARRAPA
                     ALAALAIGRLRLRARGLRHMVRREETVRRIELARLDASWTIACSLGSLDFLRGAEFQS
                     KHLLTALKAGEPKRLLRALTLEVAFAATPGPGSKRRTERLLKIADELAEKTSDDSAAS
                     LRSLTCGIAAYLQSHLPEAQKYLEEAIYLLSRRSAGAVWEMVTAQRFLVACLFFLGKI
                     RRLCEFIPPLLAEAEHNGNLYGTMCFRAGYSTLAWLAGDSVDAARAHLERARAEWDVG
                     SFQLSHFNLLLGEVFTDLYAGDGLAAHRRIERGWSETMKAQLHRIAILRVQGHHLRGA
                     LTLTASVEAQRRRQFSQARDLRTLARKLARRLARENVHRAGPLACSLTAAIEHADGAH
                     EEAGSLLRKAISGFEEQGMRLFAAASRARLGALLAGDEGALLREGAYVAFRDEAVADP
                     SRMIDVMAPGFDALAERSPGPGTTAGPRTSTGTLAGAD"
     gene            9289..12318
                     /locus_tag="VHE33_18705"
     CDS             9289..12318
                     /locus_tag="VHE33_18705"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HVT99537.1"
                     /translation="MSLSVQTDSDSSVSATPETAEAIVVGSGFGGAVAACRLAQAGIR
                     TLVLERGRRYEPNDFPALPPSDRLLPDLKRWTWASDQGLWDVVDLEEVVSIQAAGYGG
                     GSLIYANVHLRPPETVFDARWPKEYQGRALDGYFDLAGYMLDASPITEHPDLSGRLVK
                     TDQLAKVARHLGREETFFYPPLAVSHKAGKNVAGRVQEACNGCGACCSGCANSAKNTL
                     DLNYLAIAESRGAKVVTQCEVVDVEQNRDGTWTLRCFDHLTQVSRTYQSRNVFLCAGA
                     IHSTRLLARARLLPEAAQVQSMVGIGYYPNADAFGVIYDTAHEQRASVGPTITTSTVV
                     WDRDPGNQFFLVQDGGYAPALERLLGTLRAPAWVGRNRLTVAGRVTVGPSTITPLAVP
                     SGPPQILPLLSPLEDLLTAVGAGAFKDVPSQELRRHTTSFVAQLRKALLLPQIVQGTI
                     DRSITNWLRGSRLTSWLRPDGWVARALVYLHKWVLRTVYGTSDDLAERAFDALLAAAD
                     LSPSAWADRVLGYGDQAAERRTMLLAMGRDEVPGVLQYDREHDRLIADLDLFHLAPRY
                     AAEEGLMADIALSLGGELRTSPAWAFLGKPVTVHNQGGCPMSADPRFGVTTPDGRVHG
                     CDGLYVLDGSILCTSVGVNPSATITAISERNILQFIRSYKTRQYTGRSSAENELQRHD
                     SDLAWPDSEPNSEGARQYIEQRTAASAWRAHADRQSWQLTPPTGSTPPPTSQPLGLGF
                     REVMQGYYWPTVDPIAPEDPGAYLALETRGRPDFPIILTLDVSVDNLTVFFEDCEHVM
                     KVAGEVSIRLPDENGPKTYKVESGRLDLFVPRVKPYGIRPDQVERMNAQRARAGGYTT
                     QTTDDSSSTQRTMRYALSFRDDRNRPWQIRGIKLIRENPAFDAWRDTSSLFVTLHGPP
                     DAGDTMQASEVKRMPIRGAGAAHVDLDGFLFHQLPSLRISPPDTDPARAVWATLKFAT
                     FFFGTLQRIYAPEVNSMLTTLFKPHPNNVRHNPAVLR"
     gene            12315..13520
                     /locus_tag="VHE33_18710"
     CDS             12315..13520
                     /locus_tag="VHE33_18710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99538.1"
                     /translation="MSATDVIKYEITVDSPEPVKPYLRRLRGSVPGAPAVVLLHGGNT
                     SGDTYLWPNGGLAGYLADHRFDVWILEWRSSPHIVLPLLQRNALGGSVIEECRLMNLD
                     VAVEQDLVGGLRVVRENIEQADLSVLGHCFGSAAVSLAVARGLLEPFDVGSIVLSTLG
                     LFVETPWSGWIKAEDFIMERVLHNTPSCRSISPSTFDDWPTDMTVSYRRWPKVWLPSG
                     SDDPRLLLERLAFMFGQPYSIDRLHESLRGDAVAQFFGPMHLGMFLHASQIVRRGYVD
                     RFDAPDRLVSAGRAGRSPRQTTAESLDPTHFAGKRVTLLAAGDNHLWHRESIDLMYEW
                     LRRNDGGVPDRYKKHVFPGYNLQELLWGTDAPADVFPTIKAALGRRWLRKRPSQRPAE
                     REVAAVSLP"
     gene            13573..14196
                     /locus_tag="VHE33_18715"
     CDS             13573..14196
                     /locus_tag="VHE33_18715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99539.1"
                     /translation="MSKTMEHDGSVEEPGRRDWERQDYAAATADVLAAYGGELYGFLL
                     SQFRGSLSDADEAFAMFSEDFWRGLPSFQWRSSIRSWCYKLARSAASRYRRSPQNQRK
                     RRIPLSAEEQLNELAAKSRSSTLMHLRSEVKDGVRMLREHLTQEDQDLLILRVDRGLS
                     WREVAHAMADAENEDEDTIRKLEMAVRKRFEAVTKRLKALAEEAGLL"
     gene            14292..14852
                     /locus_tag="VHE33_18720"
     CDS             14292..14852
                     /locus_tag="VHE33_18720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HVT99540.1"
                     /translation="MNTKVAGARAEPDDSITDTVLRYLPELRARARFLTQDTASADDL
                     VQDAIERALTWQHRMWRDSNVRGWLNQVLRNLFITEYRRRAMTARFSVAPSLDPDGAQ
                     RAPEPADLLSTADVENALATLAPKDAVVFRMAHLEGLPYREISDRLGMVPGTVATRLH
                     RIRRKLKRSLASVYEARLISLAGSGQ"
     gene            complement(15655..16154)
                     /locus_tag="VHE33_18725"
                     /pseudo
     CDS             complement(15655..16154)
                     /locus_tag="VHE33_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985663.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(16220..16669)
                     /locus_tag="VHE33_18730"
     CDS             complement(16220..16669)
                     /locus_tag="VHE33_18730"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HVT99541.1"
                     /translation="MSTETARNSRVIDGVDAVAADDDVAAARETIAATAEAFGRAWNA
                     RDIDGVMRTATPDLRTRQPDGTTLGFMEARTAWAGYIASGATHSIAIETIDVKGQGTA
                     VVDEMITITSNGGTLLLHGVVDYRYANGEWKIEVIRTFFVPDPPQSS"
     gene            complement(16730..18430)
                     /locus_tag="VHE33_18735"
     CDS             complement(16730..18430)
                     /locus_tag="VHE33_18735"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVT99542.1"
                     /translation="MAVFGAGVAGLTAAHELIQRGFQVTVYEPRRMFGGKARSLPVPR
                     SARGQRKPLPAEHGFRLFPAFYRHIPDTLRRIPFGRNRNGVLDNLISVDRTLFASTGQ
                     RGLCFPVRVPRDRADIVSWSAAFVDATRAWVPVADSMFFIHRMLVLLTSCDRRRETEL
                     DHITWVDFMDAKRRSSNYQRYLAGGLTRSLVAMNAEEGSARTLGLILQALMFDTLSPG
                     RSSDRVLIGPTNEVWIDPWVAQLQTLGVKFVCGALRRLIVRDNRIGGAVIALSSGVER
                     EVTAGEYLLALPHEILNSIIADDVKEAAPSLARLDRLRSAWMNGIQYYLDRDVPVVEG
                     HCILVDSPWALTSISQRQFWTGTELADYGDGTVQGILSVDISDWNTPGILYGRAAKHC
                     SAEEISKEVWAQLKMHLNVGGVRILHDAAIRSWYLDPSIEFKSKREPTNLEPLFINSV
                     GTLKFRPNAETEIDNLSVAGDFVRTNTDLATMEAANEAARRAVNAILARAGSYYSTCE
                     IWPLPEPEVLRPLKTLDSRRFQRGEPHAWYDGTTNTLGEAFRGRIHGLGDRLRRLVGQ
                     "
     gene            complement(20420..20902)
                     /locus_tag="VHE33_18740"
     CDS             complement(20420..20902)
                     /locus_tag="VHE33_18740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99543.1"
                     /translation="MFVVDRYDGQNSLARFVLAAARQIALRTGTDAERFHGEAALVAY
                     LSQVDDQGTRFWTVCDRTTLNRALSIALRSLTPRERLILRLNLLERISTARIADLYRV
                     SPPTVLQSMRRSARKILIGVQDLVCDQLPIDPRELDTFMLPLSRHIEHVMQADLTISF
                     "
     gene            complement(20921..21208)
                     /locus_tag="VHE33_18745"
     CDS             complement(20921..21208)
                     /locus_tag="VHE33_18745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99544.1"
                     /translation="MPEPWSDAEVWAIFSKANSYWPTLDVSPDNFSGFLRERFNSIGD
                     IPPERLADLFLACACLNKVSGAMELMRERTFPTVLRAFRPFDESNGFAAEV"
     gene            complement(22055..22831)
                     /locus_tag="VHE33_18750"
     CDS             complement(22055..22831)
                     /locus_tag="VHE33_18750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99545.1"
                     /translation="MPENSRSQCLLCYNTIPTDAYICTECESWQLFKRRRLALALKVV
                     LVVFAPLMAWMIAEIYQSQENIRSTVEAHVTHVTDQIAALVGMAKDLQASSDSLSTNC
                     SGTAEKAANLCLSEYINRVNHINELVAQISWKTGLLPVAEEIDDVQRKWQEIWWGQVA
                     NKVRTNLVTMAYDGRLLKCQAVDFGSSECGGTLNRLIQPFRDQTSTLMCVYTLALHEH
                     LSGLYSLLPRSSERSRLLEGVNWQLKKSFCGEHANAQPSK"
     gene            23604..24758
                     /locus_tag="VHE33_18755"
     CDS             23604..24758
                     /locus_tag="VHE33_18755"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99546.1"
                     /translation="MAVDRRWVERNLGFDPIARPAPTETFAVARAASGDAQLDDLQRE
                     IIDFDSEGAEGLQFLAFTTATGLSRFTEIPWPSGLAPRTGTAPKGRAGSALPRADVLV
                     VTWTVDEGHALSRVLTPGKDSRNDYRPYTHNYASISKKMRAGCPALEAKRLGAFWTTN
                     IGNKSVVVFKSDSHMSQDGPQLPNEDVWRQIIVEVQPRLVLTTGTAGGIGKEVEVGDV
                     IVSPLVRFDCTSKFKASPFAKAHYASVAPKTKYLAEARKLFAANAGQLPKENARAPKI
                     VKTPTTALPSSVVTTDFFGFDTSDDHYHLKGLGSVSEMGDAVLGMVASKMGSNAPRWL
                     AVRNVSDPQIKADGLTLRQQAALAAQIYKGFGRWSSVCSAITCWALIAAD"
     gene            25109..26635
                     /locus_tag="VHE33_18760"
     CDS             25109..26635
                     /locus_tag="VHE33_18760"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HVT99547.1"
                     /translation="MLTRRNVYGLGGDWPAPLLWYARGVAAMKARALAEPTSWRFYAA
                     IHGFNKGLWTGAGYLKAGEAMPTSANQKRYWNQCQHGSWYFLPWHRGYLLAFEQNVRA
                     AIVALKGPADWTLPYWNYFKPDQNKLPPAFATPDWPDGKGNNPLYVPQRYGPQDDGNV
                     YVDLADVNLKAMADPDFTGVASGGAPGFGGVDTGFEHGGRTHGGIETQPHDWVHGLVG
                     GGDETRSGLMSDPRTAALDPIFWLHHANIDRLWDVWRKNPPTDQDPKAPAWLKGPASV
                     GERAFSLPKPDGEPWDYTPADMVDDHKLGYSYDDIAPAAPPDTHRRRLQRLGAPQAAT
                     AMKPGASVVTGKSVELVGASPKGVELKGSEASTAITLDVPARKKVTASLSAVARGAAA
                     TPDRVFLNLENVTGLADSTAFKVYVGVPPAADAAQHADKLAGSISLFGVTSASEPDGA
                     HGGQGLTFVLDITDIVDALHLQNALDVDKLDVRLVPLRPVHEKAQIRIGRISMFRQGH
                     "
     gene            26801..27418
                     /locus_tag="VHE33_18765"
     CDS             26801..27418
                     /locus_tag="VHE33_18765"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HVT99548.1"
                     /translation="MALAHNPPGPLALHWLVMTVAMMLPLMTTPIRHLRDRSLRNRRA
                     RGIALFLAGYFTIWLTAGFALSALEWGINILWVGSSTSFVLVGLCLALYEVSPLKQAC
                     LNRGHSHPAVEAFGRAADLDALRFGWTHGAWCVGSCWALMLLPLLFNRGHLAAMALVT
                     LWLVAEHLEGLVAPGWRLRRPRKAIQILVAESKRLVSSLASRSLS"
     gene            complement(28217..31534)
                     /locus_tag="VHE33_18770"
     CDS             complement(28217..31534)
                     /locus_tag="VHE33_18770"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HVT99549.1"
                     /translation="MVPTRRIVFRCENGCLRPYSSTAGAVAFSAVLFLCVTARATTNT
                     QPIQLQTLTNSCGANQVQNFFSITNNGTTSVKLSDLSVKFWAYDTTAAQNIAANVSAG
                     GCLTGINGNPSCTHQVSGVTVAAVKFSPACGPTTAVQANWEVSISTTDTTLLPPGATW
                     TGIQVSSHLANFANFTPGTSHWYSPCLAGFSYASDPRFGLYVAGQANTYNGVAAPICR
                     APHGQQQLSGNQTSASVMGTLIGPLSPSTTVNLAIGLPVQNTAALTAQMQAVTDPTSA
                     TYRQYLTPSQFAAQYGVSSATYQSLMAFAQAHGLTVTKTYGSNLLLDVTGTAQSVEQT
                     FYVNLFSYQRADGTRFYAPDRQPSVDTSVPLLYIGNLDTSFVMKPSAGSGSNGTFQGT
                     DFRNVYVQCSTMDGTGQSIGLLTGGGFSLGDVQSYIATTGVPRLPPITTVFATPSDMN
                     YQTCHPAPGGVDTCVANSGCQTTGSACPNEADGALETTLDIEMAASMAPGAAEIVVFE
                     GSFGDDLLHSMATRTPLILNLSSSFGFNRDASALQAIEEFVVQGQSFVTASGDSGGHT
                     TDIADKGDLSYMTWVGGTELTTTGSPPTTPPRYASETGWSSSSGWVASGNTTLDPVQP
                     PTPIPFYQTGIDMSQNGGSTQFRNIPDVAFPADLVNFVFKGQLQGVAGTSIASPLFAG
                     YLALINQYSTQQNLEPVGFINPAIYTIAKSPPLYARYFHDIISGSSGPFNATKGYDLV
                     TGLGSPRCGMIAGLSSRMPATTQPISASASSACAVRPGGTLWCWGNNVKGGLGNGSKI
                     DSLTPVQTLVSGPVVGVSSRWLHACAILATGAASCWGSNDVGELGNGSHGNGTDNPFP
                     GSGPSPVGSIAAGLVHTCVAMLDGSVKCWGQALGNGQPMEADLPVTVAGILNAAQVSA
                     GEDDTCALLFDQTVLCWGYNGVGQLGNGSTTDTTSPVMVQGINSAIMIASGMTHVCAL
                     LSNGTVSCWGDNGVGELGNGTLVNSSVPVQVSNLTDAIAIGAGSGATCAVRASGAVVC
                     WGGLPNSSGGLSTTPTPSPVPVSGISTARQVAVGDDNNFCAQLANGDVVCWGANFHGA
                     LGNGTTTDSLSPVAVQF"
     gene            32597..33619
                     /locus_tag="VHE33_18775"
     CDS             32597..33619
                     /locus_tag="VHE33_18775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99550.1"
                     /translation="MATTKFSRERLPDKLLSAKAEAVSAFLSSEPVERTLAFAATSRP
                     EHNVVGVGIGPKMVKGKPTSGYSVRFYVVSKIPLSAIKKDHVLPSKVKGMSADVVEVG
                     VFRAFAVGPTAEQKRRRPARPGSSIGFQFPGSKATFVMAGTFGAVVTDGSNRLILSNI
                     HVLADENNLSPGASIFQPGLLDGGSVNRDEIATLSKFVPLDFSAPNLVDCALAAVDRA
                     NLISAAVLPKVGKLASAVPVPAVVGMKVHKTGRTTGYRTGVVFDVSADVKVGYEGGTA
                     VFQNQVLIKGDTAQSFSDAGDSGSLIVDRATKRATALLFAGSASHTIGNHISDVLSAL
                     DVTLVV"
     gene            33665..33883
                     /locus_tag="VHE33_18780"
     CDS             33665..33883
                     /locus_tag="VHE33_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99551.1"
                     /translation="MEPNDLASRKQRLSPKLLAIDGVSGVGIGEGNIVVYLVDDSDAV
                     RKQARGIIDAEEPGTPVSFHVTGAFRKT"
     gene            34714..35943
                     /locus_tag="VHE33_18785"
     CDS             34714..35943
                     /locus_tag="VHE33_18785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99552.1"
                     /translation="MRRRTFLKTAGAGIAAGLAVGLSGRRSLAAWGDPDPSVWGGHGT
                     PATKVLEIHLLGGMAPWESFYYRPIADGTRGFDTDLDASHLKWNASCAGTPNGLASQS
                     LGVDAAGNPVNLGPFAAPLWRQDIISKTRVVVLQHDLQPHEAAIPYALSGRRLGRPGA
                     CGLGAPLQRRAHELDPTRAMPASYCLVPPIFLGSSLFSMLDAVGSHPGAARPVVLPVG
                     ATTTSFLASLTRTVPAAADPLIQQYDVQYGRRLFVTNATDRTRSAAYADFDSSTGGLQ
                     SAASLSTFLNGGPLVPANRPQCSSVTTTFDISDGFRTAAMQSAAYLLNHPTAANRARY
                     VCIVDGPFNPSGFLSYDLHHGAAPNGQAQLTSTHLWETLSGLASIIRQPTDPRSRSTC
                     HCRPACRCSRPPPGCWD"
     gene            complement(35861..37033)
                     /locus_tag="VHE33_18790"
     CDS             complement(35861..37033)
                     /locus_tag="VHE33_18790"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVT99553.1"
                     /translation="MMTRLPMSALAGVFIFHLTAAAAQAQQAWEATQPSPSKNGHGHM
                     VKDSGGNVIMTAPYNTIVKFRASDGAVLWNILWDGTSNSSFGQLAIDAGDNVYVSGTV
                     TRFDSGGNPIAVLVVTKLSPSGSIVWTGRQDLGTVSEHSSGGAIALGSNGSLYVIGTR
                     GNSSAPTAPATLVRMSTSTGAVQLPTVSLGGVIDFSADMPMERLLVDSSNNVYWSSAS
                     GLTRYSSTLGSPVTWSGHLPVRGMKISGTSVYITGPQASSSSSQMYVAKLTTGLATTW
                     KYLFRAQPRSAPYPGLGEIGTPGSDTTYGGNAVTVDSSGNVYAAGDAVTSNGSFTGGA
                     LAKFNASGQLLWAQQIGNNGLDAACFDVAVSPNNRVVVGCTDMQVGNGTSTAIADL"
     gene            complement(37059..38795)
                     /locus_tag="VHE33_18795"
     CDS             complement(37059..38795)
                     /locus_tag="VHE33_18795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99554.1"
                     /translation="MKAFGDKSRQRVLRTTICAAALYLGSAGAARAQTPSVTYHNGTV
                     INTPSVFVLFWGSFDERTEVPTAMTYLSDLAGYISNPGSTEPTIRQYGVFSASVSTFR
                     AGQGWFHDTATPPGDPPSDAQIFAEVAQLQAFGFIPPYSAQTLILVTARTSGGCSAHH
                     TTAGPNQWVGFVPYGGGLNCTVHRTTSPAKSNNELAWEAQASHEIFEAATNPDLSTGW
                     HADNVSACTDGELADCDQVASVPGCSWNGTNGDPTDFRLTFANGHQGYVAHIFDKSSR
                     GCAYRVPLGQSGQLSLAGGEDLGANLIGSPGVALSSWTGRLDVFATGTDRNVYTKHCT
                     GVCLNNWSNWTTLPAIGIGSGIFSQPAAVSWAPNRIDVVALNSVGSVMHVATTDGTNW
                     EGWDNALGGSYVRVAIDSQGPNSLDVFGMRSDGGVDVNQLKNGSWTGWLPIGGSAGRL
                     DTSRSFSATSWGTGRIDIMGFNNNTLPHDSRTSGWAPWNLVPSPVPPCNGNITLAARG
                     FGQLVAMCSTSAGSGTSSVFQSGVGWTPWRSGGLGVNTSVAASTSWNGARTDVLFTDS
                     QGHLTHGYTFIP"
     gene            complement(38815..40857)
                     /locus_tag="VHE33_18800"
     CDS             complement(38815..40857)
                     /locus_tag="VHE33_18800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HVT99555.1"
                     /translation="MAKSNEARFPFAVRENSLKRVACLAFVLGAAGCGGPTGNSEAQT
                     VQLALAKGGNARPLRRTLLGHFRPSTAQWTLEMAPPGSTSPPAAKTFVYGNPNDTPLV
                     GDWNGDGVETQGVWRQGQWLLSNTLGNGTADVTFSFGGTTDLPVVGDWDGDGKATPGT
                     YRNGVFSLRNSNSAGPADLTFGFGDPGNIPVVGDWNGDGIDTVGVYDPAHATFLLTNN
                     PAGASIADVVVPLGDVGMRPVVGDWDADGKVTIGVTLGTQRFLLNFMEQSVADVYLND
                     SSAASTDIVIAGNWSSSAVRRPGATPSKLSSFFPLGVWAQYAVDFPTWRARGINMVAG
                     VPTYALPPLYPNPETIDNWTNAANCVGQSGCTKLKMIRVPRATPSLDVGEANLLDWLI
                     EDEPDTSGEAGAAHIATTYDALNSVQGHPPISVDLVGRFLFDPTGDICGGPGDYTNAV
                     TTCYPTFLSKDDWIFHDFYPVNATGMPWGQNPYPLTDIRTVARTIDKVARWSSKPQFV
                     FVEASPRIYDPSLPGPMPGQLRAEVWLAIIHGARGITYFTYSFCHTDDQGWCPDGVTP
                     DVLVEMTAQNARITGLASMIQSPMNPSAIGFSVGRSAADWPIEATWRQFGGKTYVIVL
                     NTSPNTLSRVPMTVTGLPAGQKTLNVVGENYLVGLLFAQSFTDSFQPYQVHIYATN"
     gene            complement(40954..42762)
                     /locus_tag="VHE33_18805"
     CDS             complement(40954..42762)
                     /locus_tag="VHE33_18805"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HVT99556.1"
                     /translation="MRTSYETEAPVSSILPLRRAVALRLTALCAFAALTGVALQAFAL
                     PIPPPSKQGVRYYGYFADLRYDDAAATADQANIVCYGDFGNEEVGTRSVSELKALVAN
                     PKTKTMGVVLSVANEFFVFDQGGIPSPRATADWQSRWATYKKMIAPYMNNIVSFYLFD
                     EPSVGDVTLMNFLGVVASRIQSDFANTYPNIHRQITFTAQGLDSSTVTIPNGVDLVGY
                     DCYPGTFQECYAKEIYDPKDPANWRSVPYYYAMLRAAVARSNLPYHMNLAVIPEAMEM
                     ASGPDTSVSAQAAILARAEREVALAENDPDVVMVMPFLWDTIPSEGLVGTADLPMVKT
                     YYTALGQHFLNGSARLAYPTSVFASASYSAATGPSYAFDYSTSTMWSSGGYPIGDISA
                     VFADPIVATQMNFVVAQSPAGFTRHVLSGANAGTSAGPIATFSGSTSDNQTITWTGSR
                     PLNYVDLQTQASPSWVAWKDVSIFASGSTRIYPSPSSLASDASLAFLVDGDLATAWSS
                     GGYAPASVDLDLHAPQTLSRVELVVEQTPAGYTHHVVYGGPTLGSLSVLGSFDGNTQS
                     GQALVLSGTFTNVRYVRIQTTASPSWVAWREVNLFR"
     gene            complement(42815..45229)
                     /locus_tag="VHE33_18810"
     CDS             complement(42815..45229)
                     /locus_tag="VHE33_18810"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HVT99557.1"
                     /translation="MILDLIGRGGMGQVYAAYDPKLDRRVAVKLLRPHYLDTAVDDEA
                     QSRLLREAQAIACLSHPNVVAVYDAGTWEGHVFIAMEYVEGKTLGDWGRRQVRGWKEV
                     LEVFAGAARGLAAAHAAGLIHRDFKPENVMVSREGVARVMDFGLVRRQSNGDTGDLEQ
                     MAPQSIALTRTGALMGTPRYMAPEQLLGKPAEPRSDQFSFSVALFEALYDHRPFSGDD
                     LPTLRDAVLNGRLNLPAGRRKVPAWLRSVVLRGLSVAPERRFASMPALLAALDRGSGR
                     RRSRVLIAAAVALVAVAFSIYRIAGRQGQLCRGGTARLAAAWDTEQAGGRAPRRAAVK
                     QAFLSTGLRDAPAIWERTAGALDRYRREWADAYTETCEATQVRGEQSSQVLDLRMSCL
                     NERLEELKALTSLFAAADAAMVARSTATVEALGALDRCANVPVLLAVVPPPQAKETRA
                     AVDRLRQSLAQVKALLDVGHYGAGVAMAIPLSAEARRLGYRPLLAEVLMLLGRLQYEA
                     GDSATSQSTLAQALAEAEASRHDEVLADAATLLAGAIVETDFSRAAEAERWLTLAAAA
                     LDRMGPGHERTRGWILTERAAIASRLHDFSSAVSFSREAVRLKEKVLGSDSPDVGNSL
                     MNLDLGLVGLGEPAEALFVNDRELRIYRQAFGDDSSYVAACLSNRGEALYALGRYEDA
                     RVAFERAISTWTTVFGPEHRYLGYALTGLGRTRLATKDPHGAVAALTRALAIRLPHEP
                     NAWLVSDTRFALAQALWASGASARARAEAAAARAGLPTLPQAAPDRDEIGRWLAARSG
                     SLSVRR"
     gene            45317..46285
                     /locus_tag="VHE33_18815"
     CDS             45317..46285
                     /locus_tag="VHE33_18815"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HVT99558.1"
                     /translation="MPFDPIDVLGLEMAGQEGCYRVWRQAVQELDLGPSRHGTTFRQR
                     MEGNGGVDGARVGMLFETARSAHPELLLDLETFAAHVARTRDAIDGPFFAEDLYLAAA
                     CAARVDAAARLFRDRFGPIIRRVVAGLIPASAFRDDIEQQIYEHLLVGGRTTGEPQIQ
                     TYGGQAPLGAWLAVVAQRAVLMALRADSTRARARDGVARERLTEAVFDCAGESAVIRD
                     EARPLVKEALARAWAELPSRDRTVFHLHVVGGAGVERLGKMYRVSPSTISRWIARARA
                     ALLSKTGEVLSAELRLTPAESDSLMALVISQLDISAGDLPLPTSSG"
     gene            46826..47254
                     /locus_tag="VHE33_18820"
     CDS             46826..47254
                     /locus_tag="VHE33_18820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99559.1"
                     /translation="MKQLHLALISLVTLAPACATTTTTRTTWTDPAAAGYGRSGSVAS
                     VQEIVQRTEGNPGAGAVAGALVGGLMFGGGRGPAAVAGAAGGAAVGAAASQGTTEQRT
                     YEIVVRFDDGGYGEFVYAGYSPFRPGERVAVTPDGLTRAG"
     gene            complement(47269..48180)
                     /locus_tag="VHE33_18825"
     CDS             complement(47269..48180)
                     /locus_tag="VHE33_18825"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HVT99560.1"
                     /translation="MPLRVRIGLSSPLASRTQIDRGVNEWRQIARRMRDPTRRHHASI
                     FAGGVAFYAFLSTFPALAALVSIYGLLADPEHVRRQVDAVAGTLSPDIRALLHDQIGR
                     LTERSTGTLSIEAAGGIVVATWAATKGMRALLTGLSLSFGQDESRGFLRLNITAFSFT
                     LGAILCGAIAVGGLVVLPMALSFVGLPPVTEQLIRWARWPALTLVTLLGLAVVYHFGP
                     ARPPGRWHWITVGSAAATALWLGGSGLFSWLGARLGKGDLIDGSLAVVITMLTWFLLS
                     AYVVVLGAELNAEVQRTRGADTSPDVR"
     gene            48197..49015
                     /locus_tag="VHE33_18830"
     CDS             48197..49015
                     /locus_tag="VHE33_18830"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HVT99561.1"
                     /translation="MKGSHRFGRTQIVQVDSTSRGTLATGGRLALFWSVPALLIVGGT
                     IGYRTIERWPWFDSFYVAVITLTSIGYGDLHAYSVGGHILTIALALVGISSVAVAATE
                     LLRVVITGELRDSWARRRMRKRIDGLEQHVIVCGYGTLGRYVCAELLAGGVPVVAIDR
                     AEALAASCLAGIHFVLGEATADAILRRAGIERARALVAAAGADSENVVIALTARILCP
                     NLTIVARAEQEDAVSKLLRAGATRAASPYAIAGARIAQAALHPSAVDADPGMGR"
     gene            49159..50376
                     /locus_tag="VHE33_18835"
     CDS             49159..50376
                     /locus_tag="VHE33_18835"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HVT99562.1"
                     /translation="MATTLLLKAGIDFVSAQSLVYILILPLAGLAAAVLVLHGHGTGV
                     GADADEDELRSLRAFPHEAIRADINADVVSTAGKEETFPWRRAPIRAAAIIATVGSGA
                     AMGTEAPAAYLGVAVGAFLGDRGQRWRRLLRPAALAGGAAGVSALTGIALVGTVFMLE
                     LGRRGRVPFDLRRVLAALLGGIIGWGIDTVFGFKLIRFVVPDMAPSGAVRGLILALLV
                     GVASGGISAAAGLAIYNAKTWRASPGIRLAIGGLGALAAALALASIAAPSAAIGPGGG
                     AIVWAESADPRAWPLVGACLLRAGITIAAVAAGGCGGVFVPLLVVGDLAGRLFAPPLG
                     LGRDLAGAAGAAGGIAGGYHLPLTAVAMVLGIGGPGRAVLTCLVALLVATYAGAAADR
                     ALRGVGKRLFPEN"
     gene            complement(50430..>50865)
                     /locus_tag="VHE33_18840"
     CDS             complement(50430..>50865)
                     /locus_tag="VHE33_18840"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT99563.1"
                     /translation="GVARLTRAVLPGMRQRGFGRIINIGSIHSLVGSPFKSAYVAAKH
                     GLLGFSKAVALETAAADITVNTICPSYVRTPLVDAQIADQARARGIPEAEVISRVMLD
                     PMPKGVFIGADELAGIAAFLMSPPARNITGQAIVVDGGWTSR"
BASE COUNT         9210 a        16397 c        16291 g         8967 t
ORIGIN      
        1 aactcgaggc ggccatccgc gcttacgtcg acatcaacaa cgaaaagccg cgccccttcg
       61 tctggacgaa gaccgccgac gagatcctcg acagcattaa gcgatactgt ctactaactt
      121 ctgagacagg acactagcgg ccgaagggaa cagataccgc cgcgggacgc ctcggcttcg
      181 gcgtgagcca ctggagggcg tcaagcgcgc atacagcgga ccgggaagca ttcgtcaggc
      241 gacgtgccgt tcatgcgctc aacgaagccg ttggtgcgcg gcaccgtgca tcctcaccag
      301 cagatgacct tcgattttga cgtcacggcg cgcaggtgcc attgcggcag gacagggtcc
      361 cacagcagtc ggcaccgtcg gtgcgggtgc agggtgcacc ggcagaaccg cagcagacct
      421 tgccgagcga ggtggtgccg caggtgagtc ccaggcagca gtcgccaccc tggcagggtc
      481 gcgtggcggg taggcagcag ccgccgttga tgcattccag ctcgccacaa cagtcggtgc
      541 cgttcgccag cgtgcagcgg gcgccatagg cgccgcagca gactttgccg agcgagttgc
      601 tgctgcaggt gagcccgctg cagcaggtcg acgacgccgt gcagacgcgc gtggcgggca
      661 ggcaggcggg ggcgctggtg tcgactggat ggctgtcggc gtgcgcgtcg gcggtccgct
      721 tggcgtccgt cgtgctcttg gcgtccgtgg ggccgctgcc gtcgcgcgcg tcgtgggtgg
      781 cggcggcgtc gagccggcgc tggtccgcgg ctccgtcgcc aaccgaccga cagatcgcgc
      841 caccgtcggg cctggtgccc gtcgcgggcg cgagcaccag cggcaccgct ggcgagacgc
      901 gaccgagcgc aacgtcgacg atggcggtcc cctgccccac cacgcaacca ctggccgtga
      961 gcgcttccgc ctgcacctgt gtcgcgcccg ctgggccggg caagtagtag ccgaagtgca
     1021 gcgggcgcag cggatcgtag gggacctcgt gcgacgggac cgacgggcgc tcgggcaccg
     1081 agaagcgcac ggccgccggg gaggccaagc cggcggcaag cgacacatcc agaatcacga
     1141 gcccctcgtc cgtgcgaacg gaggtgcagg ccgacgccag aagcgcgagg aaacagccga
     1201 cgagagcact cgccgatgtg atcatggcgg gcacaccgca ccgtcgggac acgggagccg
     1261 gctgttgcca tggctcgcca gcgccgccgc cgtgacgacg ccgcccacca cgaccgcgcc
     1321 gacggcagtc cagaaccacc accgatgcgt gacgggtgta gcgcgcgacg gcggcgcctc
     1381 gaccagcacc gccgacgccg gcgacgtcac gcggccacct gtctcagact gcgccgattc
     1441 gggcggaagc ggagccagca tctgcacccg cgcgcgctgg agcgcctgca tgtcggcgat
     1501 gtacccctcg acctccgacc gctcggcggc gggcaggtcg tgcgcgtcgt gcaggtaggt
     1561 ctggaagtac gcgatcgcct gctcaggctg gcgcagcatc tgatggcagc ggccggcgtt
     1621 gcgcaagtaa gtggggtgat gcgtccgcgc gtacagatcg gtgtagatcg ccagtgcgtc
     1681 cgcgtatcgc tgttgctgga acagatcgcg ggcacgcaga tcggcgggcg ccgcgggggc
     1741 ggcggaaggc gaggccccga ccgcgaacgc catcacgacg agagtcaccc cggcgatcaa
     1801 ccgccgcctc atatcttcaa cggctccggc ttcaggtgct tcttgtgacc cggagtgggg
     1861 gccggccgcc ggcggagcgg cgtctccacg ccccctgacg gcgccacggc ggccgccggc
     1921 gcggccaggc tctccaggtg gaggcgcgcc gtgatcccgc gatcgagcgg caccaccacc
     1981 cgctcgctgc ggtagccttc cttctgcacg tccagcggct cgaccccggt ggcgcctggc
     2041 cacgagagtt ggaggggcgt cgtgcccaac cgctggccgt cacggacgcg gaccacggac
     2101 gccccaaccg gctctgtgac gatctcgacc gtgatgggca gcgggtcggg cggggctgtg
     2161 acgggcgcag agaccgtcgc cgaggatgtg acggggagac cccgccgtgg cgcgcgcagc
     2221 gccacaccga ccgccagcac ggccaacagc gtggccgcgg ccgccgcggc caccacccag
     2281 tgggaacgcc cgcccgccgc cgggcgcccc tccacctcgg aggcggtttc ggacagcgtg
     2341 gtgctgcgct ggcgcggtcg cgactccggc atcaccgcgg tggcgaaggg cgccaccgcc
     2401 gcgaccggcg tcgaaatttc tcggcccccc tccgacatga cgagcgtgcg caccggcgcc
     2461 cccggcccga tcgcacgcag cgccgcccgg agatcgtcca ccgatgcaaa ccgcgcggcg
     2521 gggtccttcg ccagcgcctg caagacgatc gcctccagaa gcaccggcac gtccacgcca
     2581 cacgcgcgca gcggcggcgg cgccacaccg atgtgcaagt gaaccagctc accccacgag
     2641 gtggaggcga acggcggtgc tccggccagc atctcgtgca gcaccacccc cagcgagtag
     2701 atgtccgcgc ggtggtcgac gtcgcgcgca tcgcggcact gctcgggtga catgtactgc
     2761 ggcgtcccca tcacgattcc ggtccgcgtg cgcaggtcgc cggcaccgtc cggcggttgc
     2821 tgcaacttag cgacgccaaa atccaggacc ttgacggcgg tgcgatccgg ccgctctgga
     2881 tccagggtca ggaacagatt gtcgggtttg aggtcgcggt ggacaatccc cttgcgatgg
     2941 gcggctccca ccgcgtccgc cacctgatcg gcgatgtcca cggattcggc aatcggcagc
     3001 cggcccgcgc ggcgcagccg ccccgccagg ctttcacccg gcagcaactc catcacgatg
     3061 tacggcgcgc cagacgccag ggtcccgaaa tccagcactt cgacgatgcc ccggtggccg
     3121 acggcgttcg cggcatgggc ctcgttgaag aagcgctgga cgatctctgg atcggcagcc
     3181 agctgggcgt gcaaaacctt gatcgcggcc ctccgcccga ggcccgggtg ctcggccagg
     3241 tagacggtcc ccatcccgcc gccacccagc agcccggtga tccggaagtt cccaacgcgc
     3301 tcaccaatca tcgcgatccg atcgacccgc cagcctagcc gacgacctcg gggagcgaaa
     3361 cggattttcg gccgccctcc tgcgaacact ctgcttgaag gtgctctggt gggcgagctg
     3421 ctgcagtcca cctccgcacc cccgcagcgg tcgcccgggg catcgtgtcg gccccagaag
     3481 cacgtctcgg ccgacacgac ggacagacat ccgcgccaac tgccgagggc gccactgttc
     3541 cagaagggac ctggtggagg attgagtgaa ggcgcgccgg cgacagtacc aggctggtct
     3601 tggcgtccgg ttcgcacggc gtgagtcatt attgaacaca gaccagtgcg ccaaaacgtg
     3661 gtggtcgcca aggcccgccg cgccaccaag ccgcgtactg agcgtcgtgg gtcgcagttg
     3721 cgcgagacgg acctcgcgcg caagatgccg gccatgaaca cccgcgtgct gtttctcgtg
     3781 gttgtcctca tgctcacgtt tgtgcctgct tcctttgctc aggcacctgc ggtcgcaccg
     3841 accacacctg ccacggtcat ctgcaaggac gggacgagtt ccaagggggg gcgcggcgct
     3901 tgctctggac acggcgggat cgatcacggg gccccgtcct ctatcggaca cacaccctcg
     3961 gccggcgccg atccgaaggc aggtccggca gagtcggccg gtgcgggcga tgttcactgt
     4021 aaggacggaa cagcgtcgaa aggcgggcgc ggcgcctgtt ctggacacgg tggcatcgac
     4081 cacggggccg cggcttcggt gccctcgtct cgcccatcgc cgactccgcc gccactttcg
     4141 atccccacgg ctggaccatc gaggaacgcg ccgtcgcgcc ccacgactcc tgcgaacgac
     4201 aacggaacac cgacggccac gtgcaaggac ggcacgacgt ccttcgcaaa acaccatcaa
     4261 ggtgcctgct cgcaccacgg tggtgtcgct cagtggctcg acggcacgaa ctagtccttc
     4321 ggcccacccg tgacgatcag acttgcaccg cagatcgaga agccggtcag ctgcccaaga
     4381 ggcgtaagat ctcccgctgc tgagcgcggg cctcggcgga gccgcttccg ccccgtccgc
     4441 tggtgacgac ggccaggtcc cgcggcctcg atccatgctt gttctggcgt tcggcgtcgg
     4501 cacccccatc gagcaaagcg cgcacggcgg cagcgcagcg gttacggacc gaccgtaaga
     4561 gcggcgtggt gccacctgcg tcggcgacgt tcggatccgc gtcgggctct aacagaaggg
     4621 tcacggtctc ggcctgagcc tctggccgcc atcctggcgc gccgggacct ccgtcgacgg
     4681 cgtactgcaa cggcacggcc tgacgccggt tggcggcgcc tacagcggcg ccgaggcgga
     4741 gtaactcgcg gacgatcttt gccccgtcgc tggcggccgc cacgtggagc gccgtgtcgc
     4801 cagcgtaaac gtaatgatca atcgccgtca gaaggtactg ccgagtggtc tgccgggtcg
     4861 cgccgaccgg cagcgcggac ttcgccaact cgggatgcgc tttcgagaga cgcgagacca
     4921 cgccgccctc accatcggcg atggcccgca tcaacaccaa aagcaggctc gtctttcgcc
     4981 gacaccgccg cccgatcccc ctggcgagtg gcacgttcca tctggtccgg ttcccgtgcc
     5041 ccactcggcc tgggcctgag ccctccgccc aagggcgcgt aggcctgcaa ccgcgaggac
     5101 tgacccgcat cgttcgtggg ctccggctgc ggtgaacgcc gcgacgtcgt cgtgtgtacg
     5161 gtcaccgtcc gtgcgtctcc ggacggctgg cctgcccggg tgtcccgctc gactctaagc
     5221 gcggctcggc gcgcctttcg cggagaagcc gcggcgcgcg cccaagttgt tggtggcatc
     5281 cagaattcgg ggtgggtttt tgcactcgta catggcacgg gttcgcggcc tactcgcgcg
     5341 taagctgcct cttcgcggcg gttctgtggc aagcgcgaat tggtcgtttc gcgatacgac
     5401 gtcagtccgc gccggcgagc gttcccgtgg acgttcttgg gcccgcggtc gttcccggac
     5461 caggggaccg ctctgccagc gcgtcgaatc cgggtgccat gacgtcgatc attctggacg
     5521 gatctgcgac cgcctcgtcc cgaaaggcga cataggcacc ttcgcgcaac agcgccccct
     5581 cgtcgccggc taggagagcg ccgagacgcg ccctcgacgc cgccgcgaag agccgcatcc
     5641 cctgttcttc aaagccgctg attgctttcc gaagaagtga gcccgcttct tcatgggcac
     5701 catcggcgtg ctcaatcgcc gcagtcagcg aacatgccag cggaccggca cggtggacgt
     5761 tttctcgggc cagacgcctg gccaactttc gagcgagtgt acgcaagtct cgagcttgcg
     5821 aaaattgtcg acgtcgttgg gcctccaccg aggcggtgag cgtcagggca ccgcgaaggt
     5881 ggtgaccctg aacgcgcaga atggcgatcc ggtgaagctg cgccttcatc gtctctgacc
     5941 aaccgcgctc gatgcgccga tgagcagcga gaccatcgcc cgcgtagagg tcggtgaata
     6001 cctccccgag gagaaggttg aagtgcgaga gttgaaagga gcccacgtcc cactccgcac
     6061 gagctcgctc aaggtgagcc cgcgcggcat ccacactgtc gccagccaac cacgccagcg
     6121 tgctgtaccc ggctctaaag cacatcgtcc cgtagagatt tccgttatgc tcggcttccg
     6181 ccagcagcgg cgggatgaat tcacaaaggc gccggatttt gcccaggaag aacaggcacg
     6241 ccacgagaaa tctctgtgcg gtcaccatct cccaaacggc gccggcggat cgacgcgaca
     6301 gcaggtagat cgcctcctcc aagtatttct gagcttccgg caggtgactt tgcaggtatg
     6361 ccgcgatccc gcaggtaagg cttcgcaggc tggccgcgga atcgtcggac gtcttctcag
     6421 cgagctcgtc cgcgattttc aggagccgct ccgttcgtcg tttagaccca ggaccggggg
     6481 tggcggcaaa cgcgacttcg agggtaagtg cgcgtagcag gcgctttggc tcgccggcct
     6541 tgagcgctgt cagaaggtgc ttgctttgaa attctgctcc tcggagaaag tctagagatc
     6601 cgagtgagca ggcaatcgtc caggacgcgt cgaggcgggc gagttcgatc cgacgaacag
     6661 tttcctcccg ccgaaccatg tgacggagac cgcgagcccg aagccgcaac ctgccgatgg
     6721 ctagggcggc gagggctgca ggagcccgtc gcgctcggct catgccgacg gccgtcagca
     6781 cctcatcgat cgcgctcatc ccacgttcga agtggccggc caagagaagc tgctcggctg
     6841 cggcgcgacg aagctcaagt gactcgtctg caggcgcatg gctcgccaaa tcgagaaaca
     6901 cctccgcggc ggccacgcct ctacctgcgt gcaccagcgc ctccgcttgt ttgcgcagga
     6961 cgatgcgtcg atcgtcgatc gacagctgag cgtgctctag cgccttctgg tacaggtcgg
     7021 cagccttcgc gaaagcgagc gagcgcatgg ctgcatcggc cgcggcaagc cagtacgagc
     7081 cagccttctc cagctcgcca gcggcttcga agtgggcagc aagctgattg agtgcggttc
     7141 cgggatctgc ctcgagcgtg cgcgccaggt gaaggtgaga gagacggcgt tcgccgtcag
     7201 tgagccgccg gagaacgatt tctcgtacgc ggtcgtgatg agtgtccacg gcatcggcgt
     7261 cggtcaggcc atcgctgcgc agtagatgtt tcgatcgcaa caagtcgacg acttggtggg
     7321 cgtcggccag gctcactcct gtagcgcgac gaacgaggcc gcgggctacc ggcccccccg
     7381 cgaccgagac gacctgcagg aatcgtcggg ccatggatga gagaggttcc atgcgcgcgg
     7441 cgaggaggtc ggtgagagtc gacggagacg gtctgtcgag ttggtccctc aaacctgcgg
     7501 tgtgagccag ctcggctata aagagcgggt gaccgcgcgc ctcgcggctg atgcgccggg
     7561 caacgccgac gcgatcaacc gacgggacgt caagcatcgc caaagcgatt tgttcgcctg
     7621 acgcctcgga caccgggccg agcgccacat tttcgagagt gggcagagat ggggatccat
     7681 ctccgtacag ggcctcgacg agtggaccgg ggttgttggc tcccccccgc tcgctgatga
     7741 ccaggaggag cgggcccgca tcggtgacgc gcagcagagg ggcgagcaga agtgcgctct
     7801 cccgatcgct ccactgagcg tcgtcgaggc agatgacgag tggcgcacgg cgccggagtc
     7861 gcgcgatgag ctccacgaag gcggccacag ccaactggcg tcggataccg cgatcggcaa
     7921 ccagctgagg atgcttaagg cgcgccaccg cgggtactgc tcccaaagcc gggaacacga
     7981 ccgtgagatc cccgatcgaa ggcggtagaa gctggacaac ctcttgctcc gggagacttt
     8041 cgaacaagcg gatcaggcaa tccaccaggc cgtcgaccgc cttgaacgcg atcgactcgc
     8101 gctcgcgaca gcggccacgg agcacaaggg ccccctgccc gcgggcgatc tctgcaaatc
     8161 gctgaagaag gacggatttc ccgatgccgg aggtcccggt aacgcgaacg actgcgggaa
     8221 ctcccgctcg ggtacgggcc agcgcgcttt gtaaggccgc gagctcatcc tcccggccga
     8281 tgaggcccgt tttaagctcg ttcgtgctac ggcgtcctcg ctcccgccgt tcgtggccga
     8341 gcgcctgaag cacttctgtg ccgctcgggc ggtcgttggg atctagggcc aacagacgac
     8401 cgcacagttc ccccagcccg tcgggtgcat ccatcggggc cccgggagtt cttcgcgcga
     8461 ccggcagttg tcccgtcagc gcctcgtgaa ggacgacgcc gaagctatac cagtcggcgg
     8521 cctctgtcgg cgcctcgccg agggcttgct cgggcgccat gtacatgggc gtgccggcgc
     8581 ggtcgccccg ctcaccgaga tccgggggtc tggcgagtcc gaagtcgagc aaaacaactc
     8641 ggttttcggc acgggttagg aggaagttgg agggcttgag atcgcgatga atcatcgacg
     8701 tggcgtgtag ggcgttgagg gccaccgcga gttgacgaaa gatctcccgg aggaatcccg
     8761 gcgcgcgccg ccgggatggt tctcggccca ggagcactgg cagcatcacg ccgtcgacca
     8821 ggtccatcgt gaagaaccac aggtcgcgat ccgagaacag ttcgtgcaac cgcaccaggt
     8881 tctcgtggac gatggcctcc gcgacccgga actcgtgttt gatctgggcg atcgactcgg
     8941 cgctcagcca tcccagcacc ttggctgcca cccgggtttg ccgctccgga tcgaacacgg
     9001 cgaaaacctg gcccatgcct cccgctccga gcttcctctc gacgatgaag cgatctccca
     9061 acgtggcccc cggctcgatg acccgccgcg ccgccaacgc cctcggcgtc tcagcgaccc
     9121 ttcggagcaa tctttcgaag gcctcgtccc tcgagtctgg catcacctcg cccccaaccg
     9181 agttgaacga tacacgattc gtggccgcga acgtaggctg gatgaaaagc gatttgttcg
     9241 ttcacatttc gcggctcacg tctcaagaat acgcaaaggg gggctgccgt gagtttgtcg
     9301 gtacagacgg acagtgattc aagcgtttcg gcgacaccgg agacggccga agcaatcgtg
     9361 gtgggaagcg ggttcggcgg cgccgtcgcc gcctgccggt tggcccaggc gggaatccga
     9421 accctggttc tcgaacgagg gcgacgctat gagccaaatg atttcccagc cttgcccccc
     9481 agcgaccgcc tcctacccga cttaaaacgg tggacctggg ccagcgatca gggcctttgg
     9541 gacgtggtgg atcttgaaga ggtggtaagc attcaagccg ccggctacgg gggcggctct
     9601 ctgatctatg ccaacgtgca cctgcgtccg ccggagacgg tgttcgatgc tcggtggccg
     9661 aaggagtacc agggacgcgc gctggacggg tacttcgacc tggcggggta catgctcgac
     9721 gcctctccca tcaccgaaca ccccgactta tcaggcaggt tggtcaaaac cgaccagtta
     9781 gccaaggtgg cgcgccatct tggccgtgaa gagaccttct tctatccgcc gctcgcggtg
     9841 tcgcacaaag cggggaagaa cgtcgctggg cgggttcaag aggcgtgtaa cggctgtggt
     9901 gcctgctgct cggggtgcgc caattctgcc aagaacaccc tggacctcaa ctacctggcc
     9961 attgccgaaa gccgcggcgc gaaggtggtc actcaatgcg aggttgtgga cgtcgagcag
    10021 aacagggacg gaacctggac cctgaggtgc ttcgaccatc tgacgcaggt cagtcgaacc
    10081 taccagtcga gaaatgtttt cttgtgcgcc ggagccatcc actccacgcg actgctcgcg
    10141 cgggcgcgcc ttttgcccga ggccgcccaa gtccagtcga tggtcgggat tggctactac
    10201 ccgaacgccg acgcctttgg cgtcatctac gacacggcgc acgagcagcg agcgtccgtg
    10261 ggaccaacaa tcaccacatc cacggtggtt tgggaccgtg accctggcaa tcagttcttc
    10321 ctcgttcaag acgggggcta tgcgcccgcg ctcgagcgtt tgctggggac gttacgcgcc
    10381 ccggcctggg tcggacgcaa tcggttgacg gtggctggcc gggtcacggt cggtccgagc
    10441 acgatcaccc cactggccgt accttccggg ccgccacaga tcctgcctct cctttctcct
    10501 ttggaggatt tgctgaccgc ggtcggggcg ggcgcgttca aagacgttcc ctcgcaggaa
    10561 ctacgccgac acacgacgtc gtttgtcgcg cagctgagga aggctctcct ccttcctcaa
    10621 atcgtacagg gaaccatcga ccgatccatc acgaattggc tacgcgggtc tcggctcacg
    10681 tcttggctgc ggccggacgg ctgggtcgcg cgcgctctgg tgtacctgca caagtgggtg
    10741 ttgcggacgg tctacggaac gtcagacgac ctggccgaac gagctttcga tgccctcctg
    10801 gcggcagcgg atctttcccc gtcggcgtgg gccgaccggg tgctcggcta cggcgaccaa
    10861 gcggcggaac gccggacgat gttgctcgcc atggggcggg atgaggtgcc tggcgtgctt
    10921 caatacgacc gtgagcatga ccggcttatc gccgacctag acctttttca cctggctccg
    10981 cggtatgcgg cggaggaagg tctgatggcg gacatcgcgc tgtcgctcgg aggagagctc
    11041 cgcaccagcc cggcgtgggc gttccttggg aaaccggtga cggttcacaa ccagggaggc
    11101 tgccccatgt cggccgaccc gcgattcggc gtgaccaccc ccgatgggcg ggttcacggc
    11161 tgcgacggac tctatgtcct ggacggatct atcctctgca cgtccgtcgg tgtgaatccg
    11221 tccgcgacca ttaccgccat ttccgagcgg aacattctac aattcattcg gtcgtacaaa
    11281 acgcgtcagt acacaggtcg ttccagcgcg gagaatgagc tgcagcgaca cgactcggac
    11341 ctggcctggc cggacagtga gccgaattcg gagggcgccc ggcaatacat cgagcagcga
    11401 acggcggcga gcgcctggcg ggcgcacgcc gaccgacaga gctggcagct caccccacca
    11461 acggggtcga cgccgccgcc gacgagtcag ccgctcggat taggatttcg tgaggtgatg
    11521 cagggctact attggccgac ggttgatccg atcgcgccgg aggacccagg cgcctacctc
    11581 gcgctcgaaa cgcgggggcg gccggacttc ccgatcattt tgacactcga cgtaagcgtc
    11641 gacaacctga cggtcttctt cgaggattgc gagcacgtca tgaaggtcgc cggagaggtc
    11701 agcatccgcc ttcctgacga gaacggaccc aagacctata aggtcgaaag cggacggttg
    11761 gacctcttcg ttccgcgggt caagccttac ggaattcgcc cggatcaggt tgaacggatg
    11821 aacgcacaac gggcccgggc cgggggctac acgacgcaga ccaccgacga cagctcgtcg
    11881 acacagcgaa caatgcggta cgcactctcc ttccgtgatg atcgcaaccg cccatggcag
    11941 attcgcggaa tcaaattgat tcgagagaac ccagcgtttg acgcctggcg tgacacgtcg
    12001 tcgctcttcg tgacgctgca cggcccacct gacgcgggcg acacgatgca ggcctcggag
    12061 gtgaagcgca tgccgatccg tggagcgggt gcagcgcacg tcgatctcga tggctttctc
    12121 ttccaccagc ttccatcgct tcggatcagt cctccggaca ctgatcccgc tcgtgccgtc
    12181 tgggcgactt tgaagttcgc gacgttcttc ttcgggaccc tgcagcggat ctatgcaccg
    12241 gaggtcaact ccatgctgac cacccttttc aagcctcacc cgaacaacgt tcgccacaac
    12301 ccggcggtgc tccgatgagc gccaccgatg tcatcaaata cgagatcacg gtcgactctc
    12361 cggagccggt gaaaccgtac ttgcgccgtc taagaggatc tgttcccggt gcgcccgctg
    12421 tcgtactgct ccacggcggc aacacaagtg gcgacaccta tctgtggccg aacggtggcc
    12481 tggccggcta tctggccgac caccggttcg acgtttggat tctcgagtgg cgcagcagcc
    12541 cccatatcgt cttgccgctg ctgcagcgga acgcgctcgg cggctccgtc atcgaagaat
    12601 gtcgcctcat gaacctcgac gtcgcggtcg agcaggactt ggtcggtggc ctgagggtcg
    12661 ttcgggagaa catcgaacag gccgatctgt ctgtgctcgg ccactgcttt ggatcagctg
    12721 ctgtcagcct ggccgtcgct cggggattgc tggagccctt cgatgtgggc agcatcgtcc
    12781 tcagcacgct gggattgttc gtcgagaccc catggagcgg ctggatcaag gcggaggact
    12841 tcatcatgga gcgcgtcctc cacaacacgc cttcctgtcg gagcatcagt ccctcgacgt
    12901 tcgacgactg gccgacagac atgaccgtct cttacaggcg ctggccgaag gtctggctgc
    12961 cgtcggggag cgatgatccg cggttgctcc tcgagaggct ggccttcatg tttggccagc
    13021 catactcaat cgaccgcctc catgagagct tgcgcggcga cgcggtcgcc cagtttttcg
    13081 gcccaatgca cctcggcatg ttcctgcacg ccagccaaat cgtgcgacgg ggatatgttg
    13141 accgattcga cgctcccgac cgtttggtct cagccggccg ggcaggcaga tcgccacgac
    13201 agacgaccgc agagtcgctc gacccgacac atttcgccgg caagcgcgtc acgcttctcg
    13261 ccgctggcga caaccatctg tggcaccggg aatcgatcga tctcatgtac gaatggcttc
    13321 ggcgcaacga cggcggtgtg cccgaccgct acaagaagca tgttttccct ggatacaacc
    13381 tgcaggaact gctctggggt acggacgccc cagccgacgt gttccccacc atcaaggccg
    13441 cgctcggacg ccgctggctg agaaagaggc cctcacaaag accggccgaa agggaagtag
    13501 ccgcggtctc gcttccctag gcgaggcggg caccgttgca ccccggctgg ccttgggcta
    13561 gcttccagga tcgtgagtaa gacgatggag cacgacggct ccgtcgagga gccagggcgt
    13621 cgcgattggg agcgacagga ctatgcggcc gcgaccgccg atgtccttgc cgcatatgga
    13681 ggcgagttat acggctttct gctgtcccag tttcgcggga gtctgtccga cgccgatgag
    13741 gccttcgcga tgttcagtga ggacttctgg cgcgggcttc cttcgttcca gtggcgttcc
    13801 tcgatccggt cttggtgcta caagctggcg cgaagcgccg ccagccgata tcggcgctcg
    13861 ccgcagaatc agcgcaagcg tcggattccg ctcagcgccg aagagcagct caacgagttg
    13921 gccgcgaaga gtcgatccag caccctgatg caccttcggt cggaagtgaa agacggcgtt
    13981 cgcatgctga gggagcactt aactcaggaa gatcaggacc tactcatcct gcgagtcgat
    14041 cgggggctct cgtggcggga ggtggcccac gcgatggcgg atgcagagaa cgaagatgaa
    14101 gatacaatcc gaaagctgga aatggccgtc cgcaagcgtt tcgaggccgt gaccaagcga
    14161 ctcaaggccc tcgcggagga ggccgggctt ctctagcgga gcagacgaag cgaggtgccg
    14221 agagaagcgg cggcgctcac attcggttcg accgctctct cccagtcatg gaacgactag
    14281 gggggacgga gatgaacaca aaagttgccg gtgcgagggc agaaccggac gactcgatta
    14341 cggacacggt gttgcgttat ttgccggaac ttcgcgcgcg ggcacgcttt ttgacccagg
    14401 acacggcgag cgctgacgat ctcgtgcagg acgcgattga gcgggcgctg acttggcaac
    14461 accgcatgtg gcgggacagc aatgttcggg ggtggttgaa ccaggtgctg aggaacttgt
    14521 tcatcacgga ataccggcgc agggccatga ccgcccgctt ctccgtcgcc ccttcgctgg
    14581 atccagacgg agcacagaga gcacccgagc ccgccgacct cctttcgaca gccgacgtgg
    14641 agaatgctct cgcgactctg gctcccaaag atgccgtggt ttttcgaatg gctcacctcg
    14701 aaggacttcc ctatcgggag atttccgatc gcttggggat ggttcccggc acggtcgcca
    14761 cgcgccttca ccggattcgc cggaagttga agcggtctct ggcgtccgtg tatgaggcgc
    14821 gcctaatctc gctggctggc tcgggacagt gaccggagcg agacgtcttg gagaagaatc
    14881 tttggaagcc ggccgtctcc gagcaaccca tccccgcggc gcggtcgcgt tgaaagcgtt
    14941 gaccctgtca atgcggctct ccaacatcgt atccgttcgg cgagtcgcgt agctgacccc
    15001 ggaggcctgg ccgtctagtt gtctgggcat aagacggaag gcgcgaaggg tggccggagt
    15061 tggtagcccg gtgggaggcg agtggcggga gtgccgcggg gctcgcgagc cgagtgggca
    15121 ctggaacagg gacgctgcac cggtcgcggc gcgagctgag gagggggggc gccggccaag
    15181 gctccggagc tctcgctggc aaagctggtc gaggtgcacc cgctggtgcg cacgaccgac
    15241 gagggttcga ggtgcggctc ggggatgggc ggagcgtggg tgcgccaccg tgcttgacgc
    15301 cgaggcgctc gggcgcttgc tacgagtgct ggaggcagcc gatgatccgt cgggcgatcc
    15361 tcgggcttct cgccctcctt acgaaagctc ttcatgtgct cgcccacgcc tcgatcaacg
    15421 tgcagtttac ggtgaaatgc ttggcgaaga ccaagccagc gcccttaggc tggcgcacga
    15481 ccttctcgaa ggattgtcga gccacctcgt gctccaccag ccggtcgctg ttcttcgtga
    15541 atatcgtggc gtctcagcac tcactgggaa acgctcgcgg tcggcacgtc gaaaaaacga
    15601 gaacgccgtc cctcacacaa cacctgagta agtcaccacc aaccaccggt ggttcatgta
    15661 gaggcctacg atatccctga tcttcgcgac gagctgaggg tccggagaca gcttgaaaga
    15721 cagcgtcctg tgcggtcgca gaccaaacgt tcgccagata cgaccgacca tcgtgtgact
    15781 gatgccgacc tgcgacgcca tcttccgggt gctccactgc gtccggccct tcggcgtcgt
    15841 ttcgagcgtc ttgatgatca gggcctccac gctctcgtcg gagatctgtc gtggtgcccc
    15901 cacccgcggc tcgtcgtaga ggccgtccag tctctgggcg ataaaccgct gtcgccacct
    15961 gcagaccgtc tggttcgacg tccggcatcg gcgagcgacc tcggcactgc tgatcccttc
    16021 agcgcacgac agaaccagct tcgcccggaa cgcgaggtgg cgattcaccc gggctctatg
    16081 cgtcagccgg acagttcctc gcgctcgcca tcggttagga cgagcgccgc tttcagagga
    16141 ccgatggtct tcatgatccg ggtagatcat gagaccacag tcccgttcaa gttatttcgc
    16201 ttccatgggg ctagctaagc taggaggact gcggcggatc ggggacgaaa aatgttcgaa
    16261 tcacttcgat cttccattcg ccgttggcgt atcggtaatc gacgacgccg tgtagaagaa
    16321 gagttcctcc attcgacgtg atagtgatca tctcgtcaac aactgcggtc ccctgtccct
    16381 tgacgtcaat cgtctctatc gcaatgctgt gtgtcgcccc tgatgcgata taccccgccc
    16441 acgccgtccg ggcttccata aagcctaggg ttgtgccatc tggttgccgg gtccgcaggt
    16501 cgggagtcgc cgttctcatg actccgtcaa tgtccctcgc gttccacgcc cgcccaaatg
    16561 cttcggcggt ggctgctatg gtttccctgg cggcagcgac gtcgtcgtcg gccgcgaccg
    16621 cgtctacacc atcgataacc ctcgaattgc gcgccgtctc cgtgctcata gctccactct
    16681 cctggtatat ggttgattcc cacgcccgct gacttaccgg tcgcgaccgc tactgtccga
    16741 caagcctacg aaggcgatcg ccgagcccgt gaatgcgccc gcggaacgcc tcgccgagcg
    16801 tattggtcgt tccgtcgtac caggcatgag gctccccgcg ctggaaccgc cggctatcca
    16861 gggtcttcag cgggcgaagc acctctggct caggtagggg ccagatctca caggtcgagt
    16921 agtatgatcc tgcccgcgct agaatcgcat tcaccgcacg ccgcgcggcc tcgtttgccg
    16981 cctccatagt tgcgagatcg gtgttggtgc gaacaaagtc cccggcgact gacagattat
    17041 cgatctcggt ctcagcgttc ggacggaact taagcgtccc cacgctgttg atgaataggg
    17101 gttcgaggtt tgtcggctca cgtttcgact taaattcgat ggacgggtcc aaataccagc
    17161 tccgaatcgc cgcatcgtgg aggattcgaa cacctccgac attgagatgc atcttgagct
    17221 gggcccacac ctccttgcta atctcttctg cagagcagtg cttggcggcg cgaccataga
    17281 ggatccctgg agtgttccag tccgagatgt caacggatag gatcccttgt acggttccat
    17341 cgccgtagtc agccaattcc gtgccagtcc aaaactggcg ctgggaaata gatgtgaggg
    17401 cccacggcga gtcgaccaag atgcagtgac cttccaccac cgggacatct ctatctagat
    17461 aatactggat gccgttcatc cacgccgaac gcagtcgatc cagccgagct agtgacggtg
    17521 cggcttcctt aacgtcgtcg gcgatgatgc tattcaggat ttcgtgcggt agcgccagaa
    17581 gatattcgcc cgccgtcact tcacgctcga ccccagatga aagagcgatg actgcgcctc
    17641 cgatgcggtt gtcgcggaca atgagacgtc gcaatgcacc gcacacgaac ttgacaccga
    17701 gtgtctgaag ctgcgccacc cagggatcta tccatacctc gttcgtcggt ccgataagga
    17761 cgcggtccga cgaccgcccc ggagacagcg tatcgaacat caacgcctgg agaataagcc
    17821 ccagcgttcg agcacttccc tcctcagcat tcatggccac caagcttctc gttagcccgc
    17881 ctgccaggta ccgctgataa ttggaggagc ggcgcttcgc gtccatgaag tcgacccagg
    17941 tgatgtggtc gagttcggtt tccctgcggc ggtcacaact ggtaagcaag acgagcattc
    18001 ggtggatgaa aaacatactg tcggcgacgg gcacccaagc gcgtgtcgcg tcgacgaacg
    18061 ctgcagacca cgacacgata tcggcgcgat cgcgtgggac gcggacaggg aaacataagc
    18121 cgcgctggcc cgtcgacgcg aagagcgtgc gatcaacgga gatgaggtta tccagaaccc
    18181 cgtttcggtt ccttccaaac ggtatgcgtc gaagggtatc tggaatatga cgatagaagg
    18241 ccgggaaaag cctgaagccg tgctcggccg ggagcggctt acgttgcccc cgggccgatc
    18301 gaggcacggg caggcttcgc gccttgccgc caaacatccg gcgcggttca taaaccgtaa
    18361 cttgaaaacc acgctggatg agctcgtggg cggcggttaa cccggcaaca cccgcgccaa
    18421 acaccgccac tgatcgttcc atttcgccct ctaacagaga aagattctgt ctaaagttga
    18481 tcgtaacagt taagtcatcg atgggacacg ttgagtgccg gggtgccgtg aatacttctg
    18541 aagcccggcc tgcgcgcgtg acaccgccca gctgtgattc caacgaaaca atgggcgaag
    18601 cagcggggtc aagacacgga gcagtggttt gtctgcgcaa acatgccagt cccagcacac
    18661 cctggttctg ctgtcatgct cggtgaacgt ccagatgccg tggccacgta gatctccctc
    18721 ggctttgaat tcaaagcgat gaggagggtc gtataccgtc aactcagttc gcaggtggat
    18781 gtcatacggg agcgcgcctc gaaaacaatg ctgcgctacg catccaacac ctcgcacccc
    18841 attcgtctcg actgacttgt agaccgggcg ccaccagtga gggtagctac tgacgtctga
    18901 aacaagatcg aagacaacac caatttccgc atcaatgtcc cactcgtcgc gaaatacgta
    18961 ctccctcccc attggtccac ctccgcgtat catcacatca agatatcgag agatcgttca
    19021 gacatcgttg agacttggtc agatctgatc accggccgcc gtgcggacct tgtgacctaa
    19081 agcctgccgt ttgcgcgaga atggcctgca agcgtgaaca agccgagcgt ttcctggaca
    19141 ctagttcctc gggcgacggc gaggcagctg tcgagcggca cctcttggga tgtgcacagt
    19201 tggtcacgga cccaaaatgc gcgctgatgt atgtactgac cgcctcagcg tcggcggtgc
    19261 ccaaggtccg cctagttgct tgaatcagat cgcgaaccgt atgagacgtc ccgtcacggt
    19321 caggggactg ccacgaacca cggccctcga ggattgcgcc gcggccccca gccaaagggg
    19381 aaagcaaagt ccagaacttt ctgggcaaga agcccagctg gacgtgagag aggcggactt
    19441 gtgtcaacga ttcgaaaagc tcctcggcga cgtcaaccaa tcctcgatgt gccgggacat
    19501 ggacagacaa cggaagtcgg agccgagcgc accggatcaa aggtctgttc cagcagacgg
    19561 caggtcacag accgtttgcg tcatagcatt ccggtccgcg ggcactcttg cgtgaagtaa
    19621 acggtagacg gtacggcgca gcccgaagct gacgataccg tcgacgaatg aagcagaagc
    19681 cactctgccc gacaaggaag ccagtgcaga cggggtgcga gctaccgggt ggctcccaac
    19741 gacagccgct ttaccgccaa agatcgctag acgcccgtct gcgtgagcgt cgaagtctcg
    19801 cgcgattact tcttgagcgg cagctaggtc actgacaccg ccaccaacga ccaccacccg
    19861 ttgtcccatc aggctccctc gtacgatctg tctcctgcac ctgtcaacaa caaaaccgaa
    19921 cagggagcgt gttgcgacgc cggatccgta acggatcgac aactcccgag cggtcattgt
    19981 gtcgatcgcc gctcaccaca gagctgacaa tgatgtgaag cagaaggcac cggcaattac
    20041 acaaaatgga gacctagtgg cctgcggcgt cgcaacagcg aacggtggct acctgctccc
    20101 ggctcgcgcg gcagccgccg cgaccaacta ggccgtcccc tgcgcgccgg ggcttgggtc
    20161 gcgcgaatgc taaccgggac tggatgagac ggccgccggt gatcgactcc cagtgaggaa
    20221 cgcgcgtcta cagcgccgcg gtggcgggtt tgcccccccg agaccagatc gaaggaatcc
    20281 atcaacgccg ccatcattca cgcagagact cgagctacgt agtgtatgga ggacgtcacc
    20341 gggaagacac attccccctg attgcggccc gaggcaccct gaatgaggaa accctgccgt
    20401 cgtgaaggta cgggtatcgt cagaatgaga tggtcaggtc cgcttgcata acgtgctcga
    20461 tgtggcgaga gagcggaagc atgaaggtgt cgagctcgcg ggggtcgatt ggtagctggt
    20521 cgcagacgag gtcttgtact ccgatgagga tcttccttgc ggagcggcgc atcgactgca
    20581 aaacggtagg cgggctaact ctatagaggt ccgcgattcg ggcggtgctg attcgctcaa
    20641 gtaagttgag gcgcaggatg agacgctcgc gaggcgttaa gctccgcagt gcaattgata
    20701 gcgcgcggtt gagggttgtg cgatcgcaga ccgtccaaaa tctcgtccct tgatcgtcga
    20761 cctgcgacaa gtaagcgacg agagcagcct cgccgtggaa gcgttcggcg tcagtcccgg
    20821 tgcgtagcgc gatctgacgg gcggctgcca acacgaagcg agcgagcgag ttctgaccgt
    20881 cgtaccgatc gacgacgaac aacgactcgg agagccgctg ctagacttca gcagcgaagc
    20941 cattggattc gtcgaaaggc cggaacgccc gcaggaccgt ggggaacgtt cgctcccgca
    21001 ttagttccat agctccgctg accttgttga ggcaggcgca cgccagaaag aggtcagcca
    21061 gtctttccgg tgggatatct ccgatactgt taaaccgctc tcgcagaaat ccagaaaagt
    21121 tgtccggcga gacgtccagt gtcggccagt aggaatttgc tttcgaaaag atggcccaca
    21181 cctcagcatc actccacggc tctggcactt cacgcccctc ccggacctca tgagacccca
    21241 atagaagaga ctattcaccc gaaaagtgcg ccaaaggccg cggtttggag acacgggggg
    21301 gtgaagacct cataaatgta aggcagcgaa cggttcgtca cgcgagactc tcctcttagg
    21361 gcgggattcg gtgatggtcg agccaaaggc tggacaggtt gccgcctccg atccgcaggt
    21421 ccatctgtac gttgcggcga tgcacgggcg ggcagcgcag gtgacgacgt cttgttcatc
    21481 ttcaggatca aacacagctc tcagggatcg gccgcgagtt ttcccggaac actgggggac
    21541 gttcgcgatt cggtggagtt ttctccggtc gaaccgaacc aggcgttcgt tgtcgccaac
    21601 ggctcggggg ccccgttcga cacagcgaac tccaggcatc gatcaggatc gcctccgccg
    21661 agtttccttg cccgaggcac gcacctactt ctggcgatta ccggcccata cgcctactga
    21721 agcaatcgct ttggcggcgc accggcgagg accagcctat tttcgagcgg tccgtttttc
    21781 acgaaaccgc ataaaattgc tgacgttctc ggagtcgaaa aaacgcttac gctggtaact
    21841 gactgggttt actttgattt tgcgtgaatg tcgcgtctgc cgccgaggct actgactggt
    21901 tcggccggca caatgcaacc tgcgatagca acgccgtaaa ggcgggccgg agaagacact
    21961 atgacagcga gacaatcttc tccgggcgaa cgcagatgtg caatcgggag caactacgat
    22021 cggctcgccc gcaatcggag actcagggaa gccatcactt cgacggctgg gcgttggcat
    22081 gctctccaca aaatgacttc ttcaactgcc aattgacgcc ttctagtagg cgagaccttt
    22141 cgctgctgcg cggcagcagg gagtacaacc ctgaaaggtg ctcatgcaga gccagcgtat
    22201 agacacacat gagagtcgac gtctggtccc ggaatggctg gataagacga ttgagtgtgc
    22261 ccccacactc actactccca aagtccactg cttgacattt caggagacga ccatcatagg
    22321 ccatcgtaac gagattcgtc cgcaccttgt ttgcgacctg tccccaccag atctcctgcc
    22381 acttccgctg cacgtcgtcg atttcttcgg caaccggtag cagccccgtt ttccaactga
    22441 tttgggcgac gagttcattt atatgattga ctcgattgat gtactcagac aaacagagat
    22501 tcgcggcttt ctcagccgtg ccgctgcaat tggtggagag cgagtccgag gaagcctgga
    22561 ggtctttggc catcccgacg agagccgcga tctggtcggt cacgtgcgtg acgtgcgcct
    22621 cgacggtaga gcggatgttc tcttgactct gatagatttc agcgatcatc cacgccatca
    22681 gtggcgcaaa gacaaccaag acaactttta gagccagagc cagccggcgc cgcttgaaca
    22741 gctgccacga ttcgcattcc gtgcagatgt atgcgtctgt cggaattgtg ttgtagcaga
    22801 gaaggcattg ggaacgcgag ttttcgggca ttcggttcga cttccccccg gagttaacgc
    22861 gcggggtccg aaacctttca ccgggaatca gcgagattcg attttgcgat gggcaggtcg
    22921 acgtcgacgt gacaggggca ccggacttta aagggcgtcc tggctccagc cggtcaccgc
    22981 gataggtcct tttgttctgt gtttcgagtc tctgcatccg cgcgtcaaac tgacctggtg
    23041 agcggtttga ttccggcaga agcgtcggac ttgcgggacg tcgctctggt ttcgcgcacc
    23101 agctgtgcgt cctcgctgcg actgggacgg gctccaagag cttgaccgcc gcgacgtagc
    23161 gctcgacatg gctgcttggc tcctcgaaca acccgtggat cattccttgg ctcacccctg
    23221 atcgacctcc acttgcaatc ccggtgatga ctaagcccga gcttccggcc tgtatgatcg
    23281 gggcagccgt gtgccgacct tgatgcgcta cgatttccca aaaatgggcg catattggtc
    23341 gttcacgcca cacgtttctc cacccgagtc gtaaccatga ggcgtgtcgg tgagctcgtc
    23401 tacctgtggg tcttaggggc gtaggacgtt gtgatgatga cagggcgacg tgaaggtcag
    23461 gcgccgatct ctttcacaag gcgcttgggg aagatcgtcg tctccagacg tttttagccg
    23521 gcggccgctg tccgcgctcc attgtcgtcg gcgacttcag acacggtcgc gacgtgtgac
    23581 aaacgctcag tgaaggaggt gcgatggcag tagatcgacg gtgggtcgag cgaaacctgg
    23641 gcttcgaccc catagcgcga ccggcgccca ccgagacgtt tgccgtggcg cgagccgcaa
    23701 gcggcgatgc ccagctggac gacctgcagc gggagatcat cgacttcgat tccgaaggcg
    23761 ccgagggcct tcagtttctg gccttcacga ccgcgaccgg tctctcccgt ttcacggaga
    23821 tcccctggcc cagcgggctc gcgccacgga cggggacagc cccgaaaggc cgggccggaa
    23881 gcgccctgcc ccgcgcagac gttctcgtcg tgacctggac agtcgacgag ggccacgccc
    23941 tcagtcgagt gcttaccccg gggaaggatt cgcggaacga ctaccgaccc tacacccaca
    24001 actacgcctc gatctcgaag aagatgcggg cgggctgtcc agcgctcgag gccaagcgct
    24061 tgggggcgtt ctggacgacg aacatcggaa acaagtccgt ggtggtcttc aagtcggact
    24121 cgcacatgtc gcaggacggt ccccagctcc ccaacgaaga tgtctggcgt cagatcatcg
    24181 ttgaagtcca gccaaggctg gtcctcacga ccgggaccgc gggggggatc ggcaaggaag
    24241 tcgaggttgg agacgtgatc gtgagtccgc ttgtgcggtt cgactgcacg tccaagttca
    24301 aggccagccc gttcgcgaaa gcgcactacg cgagcgtggc cccgaaaacg aagtacttgg
    24361 ccgaagcgag gaagcttttc gcagcgaacg ccggccaact tcccaaggag aacgcgcggg
    24421 cgccgaagat cgtcaaaacg ccgacgaccg cccttccgtc atcggtcgtc acgaccgact
    24481 tcttcggctt cgacacctcc gacgaccact accacctcaa agggctgggg tccgtctccg
    24541 agatggggga cgccgtgctc ggcatggtgg ccagcaagat ggggtcgaat gccccgagat
    24601 ggctagcggt cagaaacgtt tccgatcccc agatcaaggc tgacggactg accttgaggc
    24661 agcaggccgc gctcgctgcc cagatctaca agggattcgg acggtggagc tcagtctgca
    24721 gcgccatcac atgctgggcg ctgatcgcgg cggactgagc gatgggtgtc cgcggaagcc
    24781 gcgtggcaat cctcgaccta gtcccgcgcg tcgcggacct agttccatcg cggccgctcc
    24841 cgatgtgccc ctccgagagg acctccacgg cagtctaggc gttgttccga aaatccttgg
    24901 acgccgatgt gacccttctt agtcgaatcg aacgaaagaa gtcgggcgcc tgacgttagg
    24961 tagctgttga tactgcgggt gcgccgtctt cctcccgcca catgttgagc agtgaccgtg
    25021 agcctgcttg ctgccccgag cgggcgaaca cgaaaggcgc acagctgctc cccagactat
    25081 cgccgacgat cgatcgacgg aggatcgcat gctgacccga cgaaatgtct acggcctagg
    25141 cggtgactgg ccggccccgc tcctctggta cgcacgcggc gtcgccgcca tgaaggcacg
    25201 ggctttggcc gagcccacga gctggcgctt ttacgccgcg atccacgggt tcaacaaggg
    25261 tctctggacc ggcgcaggct atctcaaggc gggcgaggcc atgccgacct ccgcgaacca
    25321 gaagcgctac tggaatcagt gccagcacgg aagttggtac ttcctccctt ggcaccgcgg
    25381 gtacctcctc gcgttcgaac agaacgtccg ggcggctatc gtcgcgctca agggcccggc
    25441 cgactggacg ctgccctact ggaactactt caagcctgac cagaacaagc tgccgcctgc
    25501 cttcgctacg cctgactggc cggatggcaa gggcaacaac cccctctacg tgccgcaacg
    25561 gtacgggccg caggacgacg ggaacgtcta cgtcgatctc gccgacgtga atctcaaggc
    25621 catggctgat cccgacttca ccggggtcgc gagcggcggg gctccgggct ttggtggtgt
    25681 cgataccggt ttcgagcacg gtggtaggac ccacggcggc atcgagacgc agccgcacga
    25741 ttgggtgcac gggctcgtcg gcggtggaga tgaaacccgc tctggcctga tgtcagatcc
    25801 tcggacggcc gcgctcgatc cgatattctg gctccatcac gcaaacatcg atcgtctctg
    25861 ggatgtgtgg aggaagaacc ctccaacaga tcaggatccg aaagcgccgg cttggctcaa
    25921 agggccggcg agcgtcggcg agcgcgcctt ctcgctgccc aagcctgacg gggagccatg
    25981 ggactacacg ccggcggaca tggtcgatga tcacaagctc gggtacagtt acgatgatat
    26041 cgctcctgca gcccccccag acacgcacag gcggcgattg cagcgcctgg gggcgccgca
    26101 ggcggcaaca gcgatgaaac caggggcctc cgtggtcacg ggaaagagtg tcgagctggt
    26161 cggggcgagc cccaagggcg tcgagctcaa gggcagcgag gcgagcaccg cgatcaccct
    26221 tgatgtgccc gctcgcaaga aggtaactgc gagcctgagt gccgtggcca ggggcgcggc
    26281 agccactcct gaccgggtgt tcctcaacct cgagaacgtc acggggctcg cggactcaac
    26341 cgcgttcaag gtctacgtgg gcgtccctcc ggcagccgac gctgcccagc acgccgacaa
    26401 gctggcgggg agcatctcgc tcttcggcgt gaccagcgcg agcgagccgg acggcgctca
    26461 tggcggccag ggactgacct tcgtgctgga catcaccgac atcgtggacg ctctgcatct
    26521 ccaaaacgcc ctcgacgtag acaagctgga cgtgcggctc gtgccgctgc ggccggtcca
    26581 tgagaaggcg caaatcagga tcgggcgaat cagcatgttc cgccagggcc actgaccgac
    26641 gatgacccag gccgcgcgcg agcgacgtca ggtccgagtt ccgatcctgg tcgcgagcgc
    26701 cctcgcctgg gggctgctcg tcctggggcc cgcgagcagc gtggtcagag cgcactgctc
    26761 aggcgcggtg caccggctgt ccccgacgat atctttcagg atggccctcg cccacaaccc
    26821 gccgggccca ctcgcgctcc actggctcgt gatgaccgtc gcgatgatgt tgccgttgat
    26881 gaccacgccg atcaggcacc ttcgagatcg cagcctccgc aaccggcgcg cacgcggcat
    26941 cgcgctcttc ttggccggct acttcacgat ctggctaacc gcgggattcg ccctctcggc
    27001 actcgagtgg gggatcaaca tcctgtgggt ggggtcgtcg acgtctttcg tgcttgtcgg
    27061 cctgtgcctg gctctttatg aggtcagccc cctcaagcag gcctgcctca atcgcggaca
    27121 ttctcacccc gcagtggagg cattcggccg ggccgccgat ctcgacgcgc ttcgctttgg
    27181 atggacacac ggagcttggt gtgtgggctc ctgttgggcg ctcatgctgc tgccgctgct
    27241 cttcaaccgt ggtcatctcg cggcgatggc gctggtgacg ctctggctgg tcgccgagca
    27301 ccttgaagga ctggtagcgc ctggttggcg actgcgtcga ccacggaaag cgatccagat
    27361 tttggtcgcc gaatcgaagc gccttgtctc ctcactagcc tcgcggtcgc tctcctgaag
    27421 acgaacgagt gggccccctc ttccgagggc gcctcggcgt tggcttcggc ccatccccta
    27481 cccgggcgcc ggcgactaga ggcgaaagga tcttgagtac agcccttcgt cgaactccac
    27541 ttccgtgccg atgtgcgaca tccctgccgc atccgcccca ggtgggcgtt ggagctcaga
    27601 gtggaacgca agcgccgggg agattggccg atccagcagg gctacagccc agcggctgac
    27661 agcacgcagg ccacggcccg ccacctgttt cgcatgaagc cccgatctgc ccgcatgagg
    27721 catcttctcc gccagcgtcg ctagagcagc gccccgttga tgagcggacg ggcgctggag
    27781 cataaccggg atagtagttg cagccgatgg tctcggtgac gccgttgcag ccacagacat
    27841 actggattgc attgcacgac agctggccgg gccccggagt ccggggaatg cactgccctg
    27901 tcgccgtgca cccgtcggca agcttgaacc cacagaccat cccgacaggg cagtcggatt
    27961 cacgctgaca ggcgatagcc cgcgaaccgt cgacctgagc gctccctcca tcggttccgc
    28021 tttcgccgcc cgtcgcctga tggccaccga cattggtcac cgccccggct tgcccgtccg
    28081 gtgagcttcc accgttgccc gtgcgggccg cgccagcatc tctcgttcgc agcccgtcac
    28141 ccttgcacgc tacaaaagca aggcacaccc cggcgatcgt gcagatcaaa gggcgcaaga
    28201 gtgaaggtct ccgccactaa aactgaacag ccaccggcga aaggctatcc gttgtcgttc
    28261 cgttacccag cgctccatgg aagttcgctc cccagcatac cacgtctccg ttggcgagct
    28321 gagcgcagaa gttgttgtcg tctccgactg ccacctgtcg cgccgtggag atccccgata
    28381 cgggcaccgg cgatggcgtc ggcgtcgtgg acagaccgcc ggaactatta ggcaagcccc
    28441 cccagcagac aaccgccccg gaagcccgca ccgcgcaagt tgctcccgat cccgcgccta
    28501 tcgcgatggc atcagtcaga ttcgagacct gcaccggaac tgagctgttc accagggtgc
    28561 cgtttccgag ctccccgacg ccgttgtcac cccaacagga taccgttcca ttcgagagta
    28621 gcgcgcagac gtgggtcatc ccggaagcaa tcatgatggc agaattaata ccttggacca
    28681 tgaccggcga tgtggtgtcc gtcgtgctcc cattaccgag ttgaccgaca ccgttgtacc
    28741 cccagcacag gacggtctgg tcgaacagaa gggcgcatgt gtcatcttcg cccgcggaca
    28801 cctgagcggc attgaggata ccagccactg tcaccggcaa gtcggcctcc atgggctgcc
    28861 cattacccag tgcctgcccc cagcatttca cggatccgtc gagcattgcg acacatgtgt
    28921 gcaccagccc tgcggcaatg cttccgaccg gcgatggtcc ggaccccggg aatggattgt
    28981 cagttccgtt accatgcgac ccgttgccaa gttcgcctac gtcgttcgac ccccagcaag
    29041 acgccgcgcc cgttgcgaga attgcacacg cgtgaagcca cctcgaggat accccgacga
    29101 ccggcccact tacgagcgtc tggacgggcg tcaggctgtc gatcttgctt ccattgccca
    29161 gtccaccctt cacgttattg ccccagcacc acaatgtccc tcccggacgc acagcgcaag
    29221 cagacgatgc actggcactg atcggctgcg tcgtcgccgg cattcgggag gacagccccg
    29281 caatcatccc gcacctgggg ctaccgagcc ccgtgactaa atcgtaccct ttcgtggcgt
    29341 tgaaggggcc gctgcttccg gaaatgatgt cgtggaagta gcgcgcatac agagggggac
    29401 tttttgcgat cgtgtagatc gctggattaa taaaccccac cggctcgaga ttttgctggg
    29461 tcgagtactg attgattagc gctagatacc cggcaaacaa cggcgacgca atgctggtgc
    29521 cagcgacccc ttgaagctgc cctttgaaca cgaagttgac gaggtcggct ggaaacgcga
    29581 cgtcgggaat atttctaaac tgcgtcgagc caccgttttg ggacatgtcg ataccagtct
    29641 gatagaatgg tatcggcgtc ggcggttgta cgggatcgag tgtggtattg ccgctcgcaa
    29701 cccaccccga actgctcgac caacctgttt ccgacgcgta cctcggcggc gtcgtcggcg
    29761 gacttccggt ggtcgtcaac tcagtaccac cgacccaggt catataggag agatccccct
    29821 tgtcagcgat gtcagtagtg tggccaccac tgtcgccaga agcagtgacg aacgactgtc
    29881 cctgtacaac gaattcttcg attgcctgaa gagcactggc gtcgcggttg aaaccaaacg
    29941 aggaggacaa atttagaatg agcggtgttc gcgttgccat cgagtgcaac agatcgtcac
    30001 cgaaactccc ttcgaacacc acgatttccg ctgcgccagg tgccatggac gcggccatct
    30061 cgatgtctaa cgtggtctcc aaagcgccgt ctgcctcgtt cggacaggca ctgcccgttg
    30121 tctgacagcc ggaatttgct acacaggtgt cgactcctcc cggcgcgggg tgacaggtct
    30181 ggtagttcat gtctgacggc gtagcaaaaa cggtcgtaat cgggggaaga cgagggactc
    30241 ctgtggtggc aatatacgac tgtacgtcgc ccagcgagaa tcctcccccc gtcagcaggc
    30301 ctattgactg acccgtgccg tccatcgtgg agcactgcac atatacattt cgaaagtcag
    30361 tcccctggaa agtgccattg ctcccagagc cggccgatgg cttcatgacg aacgacgtat
    30421 cgagattgcc tatgtatagg agcggaacac tggtgtcgac tgatggttga cggtccggag
    30481 cgtaaaatcg cgtaccatct gcccgctgat aactgaaaag attcacataa aatgtttgtt
    30541 ccacggactg cgccgttccg gtcacgtcga gaagcagatt gcttccgtag gtcttagtta
    30601 ccgtcagacc gtgagcctga gcgaacgcca tcagcgactg ataggtcgct gatgagactc
    30661 catattgtgc agcaaattga ctcggggtta agtactggcg gtatgtagca ctggtcggat
    30721 ctgtcactgc ctgcatctgg gcagtcagcg ccgcggtgtt ttggaccggc aatccgatgg
    30781 cgagattgac cgtcgtcgaa ggggacaacg gaccgattaa cgtacccatg acacttgcag
    30841 acgtctggtt cccgctgagc tgttgctgtc cgtggggagc ccggcagatc ggtgcggcga
    30901 ctccgttata cgtgttggct tggccggcaa cgtacagccc aaaccgtgga tcggaagcat
    30961 aagagaatcc ggctagacac gggctgtacc agtgcgatgt accgggcgtg aaattggcga
    31021 agtttgccag atgtgacgac acctgaatgc cggtccaagt cgcgcctggg gggagcagcg
    31081 tcgtgtctgt cgtcgatatc gacacctccc agtttgcttg gacggcagtg gtgggcccac
    31141 acgctggtga gaacttgacc gccgcaaccg tcacgccgga cacttgatgc gtgcaacttg
    31201 gatttccgtt gattccagta aggcagccgc cagcagacac attcgcagcg atgttttgcg
    31261 ccgcagtcgt atcgtaagcc cagaacttga ccgacagatc tgagagcttc acggatgtcg
    31321 ttccgttatt cgttatcgag aagaaattct ggacttggtt ggcgccacac gagttcgtga
    31381 gcgtttgaag ttgtatcggc tgcgtgttcg ttgttgcgcg ggcggttaca cacaggaaca
    31441 acacggcgct aaacgcgacc gccccggccg tactcgaata gggccggagg catccatttt
    31501 cgcagcgaaa gacgatccgt cttgtcggga ccatgcgtgt tgaatagcac tcgacgcagc
    31561 aggcccgtcg gcggatttaa gtccgttcta tcggttggtt cttgcgcggt tgcttacgag
    31621 tttcgaaacc ttgttgctta cggccagggt gagtttcgaa accatcggat cgtagcctcc
    31681 gcaaccgccg cgcaccggga catcgcgctc ttcttggccg gctacttcac gatctggcta
    31741 atcgcgggat tcgccctgtc ggcactcgcg tgggtgatca acaccctgcg agtgggctcg
    31801 ccaatgtctt tcgtgctcgt cggcctgtgc ttggctcttt atgaggtcag ccccctcaag
    31861 caggcttgcc tcaatcgcgg acgttctcac cccgaactcg cggcagtcgg ccgggccgcc
    31921 gaccccgagg cgcttcgctt tgggtggaca cacggagctt ggtgtgtggg ttcctgttgg
    31981 ggctcatgct gctgccgctg ctcctcaacc gtcatctcac cgcgatggcc ctagtgactg
    32041 tctggctggt cgccgagtat cttgaacgac tggtagcgcc tggtcggcga gtgcgtcgac
    32101 cacagaaagc gagccagact ttagtcgtcg aatcgaagcg gctccgctcc gcactcgcct
    32161 cgccgaccgt cacttgaaga cgaacgactg gagacccttt cgatcacggg gagatcggtc
    32221 tcggacaaga aggttgctcg ctcgcacaca atccagagca gggcgtcgtg gccaccatgg
    32281 gaacgggtac ccagtgttct tccggcgaca tctgtgcctg cctcccagcc ccccggcttg
    32341 gtttcgagat cactccatct gagatgccgc gagttccaaa accggttcgg ctaagcccgt
    32401 cggaaatcgg actcgtccgc atgaaagatc gagcaggccg ttcgttcggt acggtgtcgg
    32461 actgaaccgg ttcccgagcg gtccacacgt gtaaggccca tacacacggt cagacggcgc
    32521 ggaagggcat ccgcacagcg cttaggcaca tctgcgggac ctcgggacag cgattcaccc
    32581 ttaggaggac gaacgaatgg cgacgaccaa gttctctcgg gagagacttc cggacaaact
    32641 gctttcagcc aaggcagaag cggtctcagc ctttctcagt agcgagccgg tcgaaagaac
    32701 actggccttc gcggccacct cccgccccga acacaacgtc gtgggcgtgg gcatcggacc
    32761 aaaaatggtc aagggcaagc ccacgagcgg ctactcggtc cggttctacg tcgtctccaa
    32821 aattcccttg tccgcgatca agaaggatca cgtccttccg agcaaggtga aggggatgtc
    32881 agccgacgtc gtcgaagtcg gcgtctttcg tgcatttgcc gtggggccta ctgccgagca
    32941 gaagcgccgg cgaccggctc gtccgggcag ctcaatcggg ttccagttcc ccggaagcaa
    33001 ggccacgttc gtcatggcgg gcaccttcgg tgccgtcgtg accgacgggt cgaacagact
    33061 aatcctcagc aacatccacg tcctcgccga cgagaacaat ctttcgcctg gcgccagcat
    33121 cttccagccc ggactcctcg atggagggag cgtcaaccgt gacgagatcg cgacgctcag
    33181 caagttcgtc ccgctggatt tcagtgcgcc caacctcgtc gactgcgcgc tggccgcggt
    33241 agaccgagcg aatctcatca gcgcggccgt tctgcccaag gtggggaagc tggcgagcgc
    33301 agtgccggtt cccgcggtag tggggatgaa ggtccacaaa actggacgca cgaccgggta
    33361 tcggacgggc gtcgtcttcg atgtcagcgc cgacgtcaaa gtgggctacg aaggtggcac
    33421 cgccgtgttt cagaaccagg ttctcatcaa gggagacacg gctcagtcct tctcggatgc
    33481 tggtgattcg ggatccctca tcgtcgaccg cgcgacgaag cgcgcgacgg ctctcctgtt
    33541 cgccggatcc gccagccaca ccattggcaa tcacatctcc gacgtcctgt ccgccctcga
    33601 cgtcaccctg gtggtatgac gtcgggcagc tggccgaccc atgttccggt ggtagctttc
    33661 cgggatggag ccgaacgatc tcgcgagccg gaagcaaaga ctgtctccga agctcctggc
    33721 catcgacggg gtttcaggcg tggggatcgg tgaaggaaac atcgtcgtct acctggtcga
    33781 tgactcggac gccgtacgca agcaggcccg cgggatcatc gacgccgaag agcctggcac
    33841 gcccgtcagc tttcatgtca ccggagcttt tcggaagacg tgagcgacgg aggtcgatga
    33901 ggcaacgtga gcaggtgctc tagccccaca gcaggtccgt caaagcgcga gatcgttaca
    33961 gctcagagtg gaacgcaagc accggggaga ttggacgatc cagcagggct acagcccaac
    34021 ggctgacaac acggaggcca aggcccgccg cctgtttcgc atgaaggccc catctgcccg
    34081 catgaggcat ctactccctt agcatcgtta aagccgcgtc ccgatgatga gcggacgggc
    34141 gctggagcgt aaccgggata gtcattgcag ccgatggtct ccgtcacgcc gttgcagcca
    34201 cacacgtact ggatcgcgtt gcaccacagc gggcggggtt ccggagttcg tggaatgcac
    34261 tgccctgtct ccgtgcaccc gtcggcaatc ttgtatacac tcgccatccc agcagggcag
    34321 tcggattcac gttgacagac gacaggcgcc ttgccgagcg cgttcgtcgt ggacgtccgg
    34381 ttcccgttcg cgggtgtacg tcgtcgcctc gcctaggcca ttggtccgct tggtctcggt
    34441 ccggttgacg tcgtactggc gggtggtcgc tcggccgagt tggtcggtct gcgagagcag
    34501 catcccgtcg tcgtcgaacc gccgacctcc tgcgacgtct ggcccgctgc gcggaagggg
    34561 cgcgcgcctg tgaagcagcg attgccccag aacagtgcgg aggagaggtt gttcgaatca
    34621 aaccgaagac ttctgtaagt aaggcatcgg ctgatcggaa gtttgacgag ctctcgcgtt
    34681 gtacatccac gccgcggcgg ggggagggct ctgatgcgac gacggacatt tctgaagacg
    34741 gcgggggccg ggatcgcagc gggactggcg gtcgggctgt ccgggcgaag atccctcgcg
    34801 gcatggggtg atcccgatcc gtccgtctgg ggcggccatg gaacgcccgc cacgaaggtg
    34861 ctcgagatcc acctgttggg gggaatggca ccctgggagt cgttctatta ccggccaatc
    34921 gccgacggga cacgcggctt cgataccgac ctggatgcat cccacctgaa atggaatgcg
    34981 tcctgcgccg gtacaccgaa cggcctcgcg tctcagtccc ttggcgtcga cgcagcaggc
    35041 aacccagtca acctcggacc ctttgctgcc ccgctgtggc gccaggacat catctccaaa
    35101 acacgcgtcg tcgtgctcca acacgacctc cagccgcacg aggccgcgat cccttacgcg
    35161 ctctcggggc ggcggctggg caggcccggc gcctgcgggt tgggagcacc gctgcaacga
    35221 cgcgcgcacg aactcgaccc cacgagagcg atgcccgcct cgtactgcct tgtccccccg
    35281 atattcctcg gttcctcgct gttttcgatg ctcgacgccg tgggctctca tcccggcgcg
    35341 gcccgtcccg tggtcttgcc ggtgggcgcc accaccacga gcttcctggc cagcctgacc
    35401 cggacggtgc cggcagcggc cgatcccctg attcagcaat atgacgtgca gtacgggcgg
    35461 cggctcttcg tcacgaatgc gaccgaccgc acccgttctg cggcctacgc cgacttcgac
    35521 tcgagcacgg gtggcctgca atccgccgcc tcgctgagca cgttcctcaa cggcgggccg
    35581 ctcgtgccag cgaaccgacc gcaatgctcg tccgtcacga cgaccttcga tatttccgac
    35641 ggctttcgca ccgccgcgat gcaaagcgca gcctacctcc tcaatcaccc gaccgccgcc
    35701 aaccgcgcca ggtacgtctg catcgtcgac ggacccttca acccgtcagg attcctgtcc
    35761 tacgacctcc accacggcgc tgcgccaaac gggcaggccc agctgacgtc gacccatctc
    35821 tgggagacgc tgagcgggct cgcctcgatc attcggcagc ctacagatcc gcgatcgcgg
    35881 tcgacgtgcc attgccgacc tgcatgtcgg tgcagccgac caccacccgg ttgttgggac
    35941 tgacggccac gtcgaagcag gcagcgtcca gaccgttgtt accaatctgc tgcgcccaga
    36001 gtaactgacc ggaagcattg aacttcgcca gcgcgccacc ggtgaacgac ccgttcgacg
    36061 tcaccgcgtc ccccgccgcg tacacgttcc ccgacgaatc gaccgtgacc gcattcccac
    36121 cataggtggt gtcgctacca ggagtcccga tctcgcccag acccggatag ggggcgctcc
    36181 gcggctgggc gcgaaacaga tatttccaag tggtggcgag ccccgtcgtc agcttcgcca
    36241 cgtacatctg ggacgagctg ctcgaggcct gcggtccggt gatgtagacg gaggtgcccg
    36301 agatcttcat tccccgcacg ggcagatgtc ccgaccaggt caccgggcta ccgagcgtgg
    36361 aagagtagcg cgtcagcccc gaggcgctgg accagtaaac gttgttcgac gaatccacca
    36421 gcagcctttc catcggcatg tccgcgctga agtcaatcac cccgcccagc gacacggtgg
    36481 gaagctggac agcgccagtg gacgtcgaca ttcgcaccag ggtcgccggt gccgttggcg
    36541 ccgaggaatt tcctctggtc ccgatcacat agaggctgcc gttgctcccc aaggcgatcg
    36601 ccccgcccga agagtgttcg ctgaccgtgc ccaggtcctg ccggccggtc cagacgatgc
    36661 ttcccgacgg cgacagcttc gtcacaacga gaacggcgat cgggtttccg ccgctatcga
    36721 accgcgtgac cgtcccactg acgtagacgt tgtcgcctgc gtcgatggcg agctgcccga
    36781 agctgctgtt gctggtgccg tcccagagaa tgttccagag gacagccccg tccgatgccc
    36841 ggaatttcac gatcgtgttg tagggagcgg tcatgatcac attgcccccg ctgtccttga
    36901 ccatgtgtcc atgcccgttc ttgctgggcg atggttgcgt cgcctcccag gcctgttgtg
    36961 cctgggcggc cgcagcggtc aggtgaaaaa tgaaaacgcc cgccaacgcg gacatcggaa
    37021 gtcgcgtcat catcgttcaa tctccttcaa gctgccggtt agggaatgaa tgtgtacccg
    37081 tgcgtgaggt ggccctggct atccgtgaac agcacgtccg tccgggctcc gttccacgat
    37141 gtcgaagcgg caacgctggt gttgacgccc aggccgcccg agcgccaggg cgtccagccg
    37201 acgcccgact gaaacacact gctggtccct gatccggcgc tggtgctgca catcgccacc
    37261 agttgaccga acccacgtgc cgcaagcgtg atgtttccgt tgcacggcgg aaccggcgag
    37321 gggacgaggt tccagggggc ccatccggag gttctcgagt catgcggcag cgtgttgtta
    37381 ttgaagccca tgatgtcgat gcgtccggtt ccccaagagg tggcgctgaa cgatcgggac
    37441 gtgtcgagcc ggccggccga cccgccgatg ggtaaccatc cggtccatga gccgttcttg
    37501 agctggttca cgtcgacgcc gccatcagac cgcattccga acacgtccag ggagttggga
    37561 ccttgtgaat caatcgcgac ccgaacgtaa gagccaccca gcgcgttgtc ccacccctcc
    37621 cagttggttc cgtcggtggt ggcgacgtgc atgaccgatc cgaccgagtt gagcgcaacc
    37681 acgtcaatcc ggttgggcgc ccacgagacc gcggccggct gcgagaaaat gcccgagcca
    37741 atcccgatcg cagggagggt cgtccagttc gaccagttgt tcaggcagac gcccgtgcag
    37801 tgcttcgtgt agacattccg gtcggtaccg gtcgcaaaga cgtcgagtcg gcccgtccag
    37861 gacgacaggg cgacaccagg cgagccgatg aggtttgcgc ccagatcctc gcccccggcc
    37921 agggacagct ggccggactg tcccaagggg acgcggtacg cgcagcctcg gctggacttg
    37981 tcgaagatgt gcgcgacgta gccctgatgg ccgttggcga aagtcagccg gaagtcggtg
    38041 gggtctccgt tcgttccgtt ccaggagcac ccggggaccg aggcgacctg atcgcagtcg
    38101 gcgagctctc catccgtgca ggcggagacg ttgtcggcat gccagccggt cgacaggtcc
    38161 gggttcgtcg ccgcttcgaa gatctcgtga ctggcctggg cctcccaggc cagctcgtta
    38221 ttcgacttcg cgggagaggt ggtgcgatga accgtgcagt tcaatccacc gccatatgga
    38281 acgaacccca cccactggtt cgggcccgcg gttgtgtggt gagcagagca accacccgag
    38341 gtgcgcgcgg tgaccaggat caacgtctgc gcagagtacg gcgggataaa cccaaacgcc
    38401 tggagctgcg cgacttctgc gaagatctgg gcgtcacttg gaggatcgcc ggggggagtg
    38461 gcggtgtcgt gaaaccatcc ctggcccgct cggaacgtgc tgaccgacgc actgaacacg
    38521 ccatattgcc ggatcgtcgg ttccgtcgaa cccgggttcg agatgtagcc agccaggtcc
    38581 gagagatacg tcattgctgt cggaacctcg gtcctctcat cgaaggagcc ccagaacaac
    38641 acgaacaccg acggtgtgtt gatgaccgtg ccattgtgat acgtcacgga tggtgtctgc
    38701 gcgcgcgccg ctccggcgct gccgaggtac agcgccgcag cgcagatcgt cgtccgcagg
    38761 acgcgttgcc tgcttttgtc gccaaaggct ttcatgggtg atctccttgg tcgttcagtt
    38821 cgtcgcgtag atgtggacct ggtacggctg aaacgagtcg gtgaacgact gggcgaacag
    38881 caaccccacc agatagttct cgccgaccac gttgagggtc ttctgccccg ccggcaggcc
    38941 ggtgaccgtc atgggcaccc ggctcagcgt gttcggcgag gtgttgagca cgatgacgta
    39001 ggtcttgccg ccgaactggc gccaggtcgc ttcgatcggc cagtccgcgg cggacctgcc
    39061 gaccgaaaac ccgatcgcgc tggggttcat cgggctctgg atcatcgagg ccagaccggt
    39121 gatccgggcg ttctgggcgg tcatctcgac caggacgtcg ggcgtgacgc cgtccgggca
    39181 ccagccctgg tcgtcggtgt ggcagaacga gtaggtgaag taggtgatac cccgcgctcc
    39241 gtggatgatc gccagccaca cctcggcccg cagctggccg ggcatggggc ctggcaagct
    39301 ggggtcgtag atccgggggc tcgcctcgac gaagacgaac tggggcttcg acgaccagcg
    39361 agcgaccttg tcgatggtcc gcgcaacggt ccggatgtcg gtcagcggat agggattctg
    39421 gccccacggc atcccggtcg cgttcaccgg gtaaaagtca tggaagatcc agtcgtcctt
    39481 cgacagaaag gtcgggtagc aggtcgtgac cgcgttcgtg tagtcgcctg gcccgccgca
    39541 aatgtcgccg gtggggtcga acaggaatcg cccgaccagg tccaccgaga tcggcggatg
    39601 cccctggacg ctgttgaggg catcgtaggt cgtcgcgatg tgcgcggcgc ccgcctcacc
    39661 cgacgtgtcc ggttcgtcct cgatcaacca gtccagtaag ttggcctcgc ccacgtcgag
    39721 cgagggggtg gcccgaggca cgcggatcat cttcagcttc gtgcagccgc tctgaccgac
    39781 gcagttcgcc gcgttggtcc agttgtcgat cgtctccggg ttcggataca ggggcggcag
    39841 cgcgtaggtg gggacgcccg cgaccatgtt gatgccccgg gcgcgccagg tagggaagtc
    39901 gactgcgtac tgtgcccaga cgccgagcgg gaagaagctc gatagcttgg agggcgtcgc
    39961 ccccggcctg cgcaccgccg acgagctcca gttccctgcg atcacgatgt ccgtgctggc
    40021 ggcggagctg tcgttcaggt acacgtcggc gaccgactgc tccatgaaat taaggagaaa
    40081 gcgctgagtg ccgagggtga cgccgatggt gaccttgcca tctgcgtccc agtcgccgac
    40141 caccgggcgc atgcccacgt cgccgagcgg aacgaccacg tctgcgatgg aggctccggc
    40201 cgggttgttg gtcagcagga aggtcgcgtg cgcgggatcg tagacaccga ccgtgtcgat
    40261 cccgtcgccg ttccagtcgc cgacgaccgg aatattgccg gggtcaccga agccgaaagt
    40321 gaggtcggcc gggccggcgc tgttgctgtt gcggagcgag aacaccccgt tccggtaggt
    40381 cccaggcgtc gccttgccgt cgccgtccca atcgcccact accggcagat cggtcgtgcc
    40441 gccaaagctg aacgtcacgt ccgccgtgcc gttgcccagc gtgttcgaca gcagccactg
    40501 cccctgccgc cagaccccct gggtctccac tccgtcgccg ttccagtcgc caaccagcgg
    40561 cgtgtcgttg gggtttccgt aaacgaaggt cttggcagcc ggcggcgacg tggacccagg
    40621 cggtgccatt tcgagcgtcc actgtgccgt cgagggtcga aagtgaccca agagggtccg
    40681 tcgaagcggc cgggcattgc cccccttcgc gagcgccaat tggaccgtct gcgcctccga
    40741 gttgccggtc ggcccgccgc acccggccgc cccgagcacg aacgccagac acgcgaccct
    40801 cttcaaggaa ttttcgcgga ccgcgaaagg gaaccgcgcc tcattgctct tcgccatgaa
    40861 gaggagatgc gcgctgcggc ggcttttgcg ggctcgggcg agaaaggttc gtccggccac
    40921 cggcgcgacg gcgcccgacg agctccgcgg ctgtcagcgg aacaggttga cttcacgcca
    40981 tgcgacccag gacggactcg ccgttgtctg aatgcgcacg taacgaacat tcgtgaaggt
    41041 acccgacaag accagcgcct ggccggattg ggtgttgccg tcgaacgagc cgaggacgct
    41101 gagcgagccc agcgttggtc caccgtagac gacgtgatgg gtgtagccgg cgggcgtttg
    41161 ttcgactacc agctccacac gcgacaaggt ctgcggcgcg tgcagatcca gatcgacgct
    41221 agccggcgcg tagccgccgg agctccacgc ggtagcgaga tctccgtcga cgaggaacgc
    41281 cagcgaggca tcgctggcca gagacgaagg cgatgggtag atgcgggtcg agccgctcgc
    41341 gaaaatcgaa acgtctttcc aggcgaccca tgacgggctg gcctgtgtct gcaggtcaac
    41401 gtagttgagc gggcgcgaac cggtccaagt gattgtttgg ttgtcagaag tcgaacccga
    41461 aaaggtcgca atcggaccgg ccgacgttcc ggcatttgcg cccgacaaga cgtgcctcgt
    41521 gaatccggcc ggggactggg cgaccacgaa gttcatctgc gtagcgacga tgggatctgc
    41581 gaatacggcc gaaatgtcgc cgattgggta accgccgctg ctccacatcg tggacgtgga
    41641 atagtcgaag gcatagctgg gcccggtggc cgccgagtaa ctggcactgg caaagaccga
    41701 cgtcgggtag gccaaccgcg cgctgccgtt caggaaatgt tggccaagag ccgtgtagta
    41761 cgtcttgacc atcggcaagt cggcggtacc caccagtcct tcgctcggga tggtgtccca
    41821 gagaaacggc atgaccatca ccacgtccgg atcattctcc gcgagcgcca cctcgcgctc
    41881 cgcccgggcg aggatggcgg cctgggcgct caccgaggtg tcagggccgg atgccatctc
    41941 catcgcctcg ggaatcaccg ctaggttcat gtggtacggg aggttgctcc gagcgaccgc
    42001 cgcgcgaagc atcgcgtaat agtagggaac cgaccgccag ttggccggat ccttcggatc
    42061 gtagatctcc ttggcgtagc actcctggaa agtacccggg tagcaatcgt agccgacgag
    42121 gtcgacgccg ttcggaatcg ttaccgtgga cgaatcgaga ccttgcgcgg tgaacgtaat
    42181 ctgtcgatga atgttcggat acgtgttcgc gaagtcactc tggatccgtg acgccacgac
    42241 gccgaggaag ttcatcaagg tgacatcgcc cacggatggc tcgtcgaaca agtagaagct
    42301 gacgatgttg ttcatatatg gagcgatcat tttcttgtac gtggcccatc ggctttgcca
    42361 atccgccgtt gctcgcggag aaggtatccc cccttgatcg aacacgaaaa actcgttggc
    42421 caccgacaga acaacgccca tggttttggt cttcgggttg gcgaccagcg ctttgagctc
    42481 cgagactgac ctggtcccga cctcttcgtt gccgaagtcg ccatagcaga cgatgttcgc
    42541 ctggtctgcg gtcgccgcgg catcgtcgta ccggaggtct gcgaagtaac cgtagtaacg
    42601 cacgccttgc ttgctcggcg gcggaatcgg cagcgcgaac gcctgcagag ccacacccgt
    42661 gagggcggcg aaagcgcaga gcgccgtcaa gcgcagcgcg acagcccgtc gaagcgggag
    42721 aatggaagag accggcgctt ccgtttcgta cgatgtcctc atgtctcaga gatgcgccgg
    42781 tccgggcgat ttgcacgcag gtcacaaacg ataactatcg tcggacggac agggagccac
    42841 tgcgggcagc caaccagcgg ccgatctcgt cgcgatcggg cgcagcctgg ggcagggtcg
    42901 gcaaccccgc ccgggcggcg gcggcctcgg cgcgggcgcg ggcggaagca ccgctcgccc
    42961 acaacgcttg cgctagcgca aaacgagtgt cggagaccag ccacgcgttc ggctcatggg
    43021 gaaggcggat ggccaacgcc cgcgtcagcg ccgcgacggc accatgggga tccttggtgg
    43081 ccaagcgtgt gcggcccaag ccagtcagcg catagcccag gtagcgatgc tcgggcccga
    43141 agacggtcgt ccacgtggag attgcgcgct cgaaagccac gcgcgcatcc tcgtaccggc
    43201 cgagcgcgta gagcgcctcc cctcgattac tgagacaggc ggcaacgtag gagctgtcat
    43261 ctccgaaggc ctgtcgataa attcgcagct cgcggtcgtt tacgaacagc gcttcggctg
    43321 gctccccgag gccgaccagc cccaggtcga gattcatcag ggagttgccg acgtcgggac
    43381 tgtccgatcc cagcactttc tctttgagcc gcacggcctc tcgggaaaac gacacggccg
    43441 agctgaagtc gtgcagcctg gacgcgatgg cggcccgttc ggtcaagatc cagcctcggg
    43501 tccgctcgtg tcccggtccc atccggtcca aggccgctgc ggccagggtc aaccagcgct
    43561 cggcctcggc ggcgcgggag aaatccgttt ccacgatcgc gcccgccagt agcgtggcgg
    43621 catcggctag cacctcgtcg tgacgagagg cctcggcctc cgccagcgcc tgggccaggg
    43681 tcgactgcga cgtcgctgag tcgcccgcct cgtactgcag gcgaccgagc agcatcagca
    43741 cctccgccaa gagcggccgg tagcccaagc gcctagcttc ggcggacagc ggaatcgcca
    43801 tcgccacccc tgccccgtag tggcccacgt ccaacagggc cttgacctga gcgagcgact
    43861 gacgaagccg gtccacggcc gcacgcgttt ctttggcctg cggcggcggc acgaccgcca
    43921 acagcactgg aacatttgca cagcgatcga gcgcgcctag cgcctccacc gtggccgtcg
    43981 accgggcgac catggcggcg tcggccgccg cgaagaggct cgtcaacgcc ttcagctcct
    44041 cgagccgctc gttgagacag gacatccgga ggtccaagac ctgcgaagac tgctcgccgc
    44101 ggacctgagt ggcctcacag gtttctgtgt aggcgtccgc ccattcccgc cggtagcgat
    44161 cgagggctcc ggcagtccgc tcccaaattg cgggcgcgtc gcgcaaaccc gtcgacagga
    44221 aggcctgctt caccgctgcg cgccgcggag cccgcccgcc cgcctgctcg gtgtcccacg
    44281 cggccgccag ccgcgcggtg ccgccgcggc agagttgccc ttgccgcccg gcgatccggt
    44341 atatcgagaa cgccacggcg accagagcca ccgctgcggc gatcagcact cggcttcgac
    44401 gcctaccgct cccccgatcg agtgccgcca gcaaagcggg catggaagcg aagcgccgct
    44461 cgggcgcgac cgacaggccg cgtaggacga cggaccgcag ccaggccggg accttccgcc
    44521 gcccggccgg aagattgagg cgcccgttca agacagcgtc tcggagtgtg ggcaggtcat
    44581 cgcccgagaa cggacggtga tcatagaggg cctcgaacag tgccaccgag aagctaaatt
    44641 ggtcggaccg cggctcggcc ggtttgccca gcagctgctc gggcgccata tagcgcggcg
    44701 ttcccatcag ggcgccggtg cgggtcaaag cgatcgattg cggggccatc tgctccaggt
    44761 cacctgtgtc gccgttggac tgccgccgca ccagtccgaa gtccatgacg cgcgcgacgc
    44821 cctcgcggct gaccatcacg ttctccggct tgaagtcacg gtggatcagt ccggcggcgt
    44881 gggcggcagc cagaccgcga gccgcgcctg cgaatacttc caggacttcc ttccatcccc
    44941 ggacctgccg ccgtccccag tcccccagcg tcttcccttc gacgtattcc atcgcgatga
    45001 agacatgccc ttcccaggta ccggcgtcgt agacagccac cacgttcgga tgcgacaagc
    45061 acgcgatcgc ctgcgcctcg cgcaagagtc tgctctgcgc ttcgtcgtct accgcggtgt
    45121 cgaggtaatg cgggcggagc aacttgacgg cgacgcggcg gtcgagcttc gggtcgtagg
    45181 ccgcgtagac ctggcccatg ccgccgcgcc cgatcaagtc gaggatcacg tagcgcccga
    45241 cctccgcgcc acgcctcaga gttaggtcgc ctgacgccgt ggccaagagg tcgagacagc
    45301 tcgagcagag accaacgtgc cgttcgatcc gatcgatgtc ctggggctcg agatggccgg
    45361 acaggaaggc tgttatcgtg tgtggcgcca ggcagtccaa gagcttgacc tcgggccgtc
    45421 gcgccatggt acgactttcc ggcagcgcat ggaggggaac gggggtgttg atggcgcgcg
    45481 tgtcggtatg ttgttcgaga ccgcacggtc cgctcatcca gagctgcttc tcgacctaga
    45541 gacgttcgcg gcccatgtgg cgcggacgcg cgatgcgatc gacggcccgt tcttcgccga
    45601 agatctctac ttggcggcgg cctgcgcggc acgcgtcgac gcggcagccc gcctctttcg
    45661 cgatcgattt ggcccgatta tccggcgcgt agttgccggg ctcatcccgg cctcggcctt
    45721 tcgcgacgac atcgagcagc agatctacga gcatctcctg gtcggtgggc gaaccaccgg
    45781 ggagccgcag attcagacct acggcggtca agcgccgctg ggagcgtggc tggccgtagt
    45841 ggcgcagcgg gcggtcctca tggccctgcg tgccgacagc acacgcgcga gagcccgcga
    45901 cggcgtcgcg agagagaggc tgaccgaggc ggtctttgat tgcgccggtg agtccgcggt
    45961 catccgcgac gaggcgcgac cgctggtgaa ggaggcgctg gcgcgcgcgt gggccgagct
    46021 tccgtcccgc gaccgaaccg tgttccatct gcacgtcgta ggcggcgccg gtgtcgagag
    46081 gttggggaag atgtatcgag tgagcccctc gacgatttcg cggtggatcg cgcgcgcgcg
    46141 cgcagcgctt ctgagcaaga caggagaggt tctgtccgcc gagctgcgcc tgacacctgc
    46201 cgagagcgat tccctcatgg cgctcgtcat cagccagctg gacatcagcg ccggggacct
    46261 tccactcccg acttcgtctg gctgactgcg ctgagttgtt ccaggtgcgc tatccgttgg
    46321 tcgatctccg gttggcgcca tcgagaagtg gttgtctgat tcgccgtccc tcagagcatg
    46381 gcacagaggt cgtggacggc ctcagtcagg tcgaacggct cgcagcaatc gccgcggtca
    46441 ctcgacttcg atcacgcctg ggcagcgcca gtggccgcgg tgccagcgcc cgcgcgggcc
    46501 gtagtggccc tcgatccaga cgccgcccgc gcaatggccg ggcaccgtcc taacgtagca
    46561 cccgctgacg agcgccagca cggttgccaa cccgaacatc gcgaccgctc gtctcatcga
    46621 gaatcatttt ctctcgcgcg ggcgcgcgca gtcaaattgc cgtgtcgccc gcggaatgat
    46681 cggcgtccgg gtcgacccat tctctcgggg cgtcgccgca gagccgaatc agcttctgcc
    46741 gtccttacgg ggagagaacc cggcgctcaa catcctcaca ggtagcgtcg gaaccaggcg
    46801 catctcttga tggagcccac gcgtcatgaa acagcttcac cttgctctca tctcgctggt
    46861 cacgctggcg ccggcatgcg cgacgacgac aacgacgcgc acgacctgga cggatccggc
    46921 tgcagcggga tacggacgat ccggttcagt cgcgtccgtc caggagatcg tccagcggac
    46981 cgagggcaac ccgggcgcag gcgccgtggc aggcgcgctg gtcggggggc tcatgttcgg
    47041 aggcggacgc gggccggctg ccgttgccgg ggcagcgggc ggggcggcgg tcggtgcggc
    47101 ggccagtcag ggcacgacag agcagcgcac gtacgagatc gtcgtccgct tcgatgatgg
    47161 tggctatggc gagttcgtct acgcgggata ctcgccgttc cggcccggcg agcgtgtcgc
    47221 cgtgacgccg gacggtttga cgcgcgccgg gtgagcccgt cagcggcttc atcgaacgtc
    47281 cggactggtg tcggcgcccc tggtacgttg cacctcggcg ttcaactcgg cgcccaggac
    47341 gaccacgtag gccgacagga gaaaccacgt cagcatggtg atcacgacgg cgagcgaccc
    47401 gtcgatgagg tcgcccttgc cgagccgggc gccgagccag gaaaacaggc ccgagccccc
    47461 cagccacaga gcggtcgccg ccgcggatcc gacggtgatc cagtgccacc gccccggtgg
    47521 ccgggcgggc ccgaaatgat agaccacggc tagtccgagc agcgtcacga gtgtcagcgc
    47581 tggccagcgc gcccagcgaa tgagctgttc cgtcaccggc ggcagcccga cgaacgagag
    47641 cgccatcggc aacaccacga gaccgccaac ggcaatggcg ccgcagagga tcgcccccag
    47701 ggtgaacgag aatgccgtga tgttgagcct caagaagccg cgactctcgt cctgtccgaa
    47761 cgagaggctc agccccgtca gcagcgctcg catccccttc gtggccgccc aggtcgcgac
    47821 gacgatgccg cccgcggcct cgatgctcag ggtgcccgtg gagcgctccg tgagccgccc
    47881 aatctggtcg tggagcaggg cccgtatgtc agggctcagc gttccagcca cggcgtcgac
    47941 ctggcggcga acatgctcgg ggtccgccag aaggccgtag atcgacacca gcgccgccag
    48001 cgcagggaag gtggagagaa acgcatagaa cgcgacgccg ccggcgaaga tcgatgcatg
    48061 gtggcgccgg gtgggatccc gcatgcgacg cgcgatctgc cgccattcat tcacgccgcg
    48121 gtcaatctgg gttcgcgagg cgagcggaga actgagcccg atcctgactc gcaggggcac
    48181 cacaggaccg gcgaggatga agggttcaca tcggttcggg cgtacccaga tcgtacaggt
    48241 ggactcgacc tctcgcggca cgctggcaac gggtggccga ctcgcgctgt tctggagcgt
    48301 gcccgcgctg ctgattgtcg gtggaacgat cggctaccgg accatcgaac gctggccttg
    48361 gtttgattcg ttttacgttg cggtcatcac cctcacgtca atcggctacg gggacttgca
    48421 cgcctattcg gtcgggggcc acatcctgac gattgcgttg gcgctcgtcg ggatctcatc
    48481 agttgccgtc gcggccaccg agctcttgag ggtcgtcatc acgggtgagt tgagggactc
    48541 gtgggcgagg agacgtatga gaaagcgaat tgacgggctc gaacagcacg tgatcgtgtg
    48601 cggctacggc accctcggcc gatacgtctg cgcggagctc ctcgcgggcg gcgtcccggt
    48661 cgtcgcgatc gatcgcgcag aagcactggc tgccagctgc ttggccggca tccatttcgt
    48721 gctcggtgag gcaacggccg acgccattct gaggcgagca ggtatcgagc gcgcccgcgc
    48781 gctggtcgcc gctgcgggcg cagattccga gaacgtcgtg attgcgctga cggcccggat
    48841 cttgtgcccg aacctgacga tcgtcgcgcg tgcggagcaa gaggacgccg tatcaaagct
    48901 tctccgtgcc ggcgccacgc gcgcagcctc cccgtatgcc atcgcgggcg cacgcatcgc
    48961 ccaggcggca ttacacccgt cggccgtcga cgccgacccc gggatgggga gatagcggtc
    49021 gtgcgctgga gctctgcccg tggtcgcggg gccgctgcgc cagcgccggc cccgagcgcc
    49081 gcgaaggcgc gcctcggggc ctggggcatc gtgggggcgg gggccacgct gggcggcttg
    49141 gccggaggct ttttcgtcgt ggccaccacg ctcttgctaa aggccgggat cgacttcgtc
    49201 tccgcccaga gcctcgtcta catcctcatc ctgccgctgg ccggcttggc cgccgcggtc
    49261 ctcgtgctgc acgggcacgg cacgggcgtc ggggccgatg cggacgagga cgagttgcgg
    49321 tctttgcggg cgttccccca cgaggccatc cgcgcggaca tcaacgccga cgtggtgagc
    49381 accgcgggca aagaggagac gtttccctgg cggcgggcac ccattcgagc cgccgccatc
    49441 atcgccacgg tgggctcggg cgcggcaatg gggacggagg cacccgccgc ctacctcggt
    49501 gtcgcggtcg gcgcatttct gggggaccgc ggccagcgct ggcggcgact tctccggccc
    49561 gccgcgctgg cgggcggcgc cgccggggtc agcgcgctca ccggaattgc gctggtcggg
    49621 acggtgttca tgctggagct cggccggcgc gggcgagtgc cgttcgatct gcgacgggtg
    49681 ctggcggcgt tgctgggcgg gatcatcggc tgggggatcg acaccgtctt cggcttcaag
    49741 ctgattcggt tcgtcgtccc ggacatggcg ccatcgggcg ccgtccgcgg gctgattctg
    49801 gcgctgctgg tcggggtcgc gtctggcggc atcagcgccg cggccggatt ggccatttac
    49861 aacgcaaaga catggcgagc ctcgcctggc atcaggttgg cgatcggggg attgggagct
    49921 ctcgcggccg cgctggcgct cgcaagcatc gcagccccct ccgcggcgat cggtccgggc
    49981 ggaggcgcca tcgtgtgggc cgagtccgct gacccccggg cctggcccct ggtcggcgcg
    50041 tgtctcctgc gggcgggcat caccatcgcc gcggtcgcgg ccggcggctg cgggggcgtg
    50101 ttcgtaccgt tgctggtggt tggcgacctg gccggccggt tgttcgcacc gccgctggga
    50161 ctcggacgcg atctcgcggg cgcagcggga gcagccggcg gcatagcggg cggataccac
    50221 ctgccattga cggcagtggc gatggtgctt ggcatcggcg gcccgggccg cgccgtcctg
    50281 acgtgcctcg tcgccctgct cgtcgctacg tatgcgggcg cggccgccga tcgagccttg
    50341 cgcggggtag gcaaacgcct gtttcccgag aactgacgaa cgagcgctgg ccgaacccac
    50401 gacggcggtc tctcgatgac cgccgaccgt caccgagaag tccatccgcc gtccacgacg
    50461 atcgcctgcc ccgtgatgtt gcgggcaggc ggcgacatca agaacgcggc gattccagcc
    50521 agctcgtcag cgccgatgaa tacacccttc ggcatggggt cgagcatcac gcgactgatg
    50581 acctcggcct ccggaatgcc gcgcgcgcgc gcctgatccg cgatctgagc atcgaccagc
    50641 ggcgttcgaa cgtagctggg acagatcgtg ttgacggtga tgtccgccgc ggccgtctcc
    50701 agcgccacag ccttcgaaaa gccgagcagc ccgtgcttgg ccgcgacata ggcgctcttg
    50761 aatgggctcc cgaccaggga gtgaatgctg ccgatgttga tgattcgacc gaagccccgc
    50821 tgtcgcatgc caggaagcac cgctctcgtg agtcgcgcga cgccg
//
LOCUS       DASZYN010000729         1835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1260726, whole genome shotgun sequence.
ACCESSION   DASZYN010000729 DASZYN010000000
VERSION     DASZYN010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260726"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..947
                     /locus_tag="VHE33_18845"
     CDS             195..947
                     /locus_tag="VHE33_18845"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HVT99564.1"
                     /translation="MFLPLILLALCFAALCWFLRNDLTEYAAFKKLTDTRDRQRRIRV
                     WILRSFLLFFAGTAVCLAVLRRLKAPFVFPSEFLPLAARFHAAIPGNHAHASQVLLPF
                     MIGAGGGVLIGILLALLFARRFRPARLGDIDALMPRNRAETLWMALISVNAGVSEELF
                     FRLLLPLLLTLATRSALTSFLIAAVIFGLVHLYQRLPGILATTLIGLALSAVYLITGN
                     LWIAVLLHIVLDLIGVVVRPSLERWLRPAARR"
     gene            1023..1289
                     /locus_tag="VHE33_18850"
     CDS             1023..1289
                     /locus_tag="VHE33_18850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99565.1"
                     /translation="MRICIKIGDRTWCFNIPILLYPLHPPKPDPEKEYGELIYDATIL
                     ASVNAAAKQLSDAGVREALNTGVAHAVAAAQKKAGANVTLSLER"
     gene            complement(1346..>1835)
                     /locus_tag="VHE33_18855"
     CDS             complement(1346..>1835)
                     /locus_tag="VHE33_18855"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT99566.1"
                     /translation="VLVNNAGVYEFAPIGDVSEQHFHRMFDTNVLGPILATREAAKYL
                     GEGGSVINVGSVVSKSATPATVVYSATKGALDTVTRVLASELGPKKIRVNSINPGPVE
                     TEGTHAAGFIGSDFMKGLIERTPLGRIGKPDDFAPIAVFLASDESGWLTGETLVVSGG
                     LQ"
BASE COUNT          324 a          694 c          429 g          388 t
ORIGIN      
        1 ccgacccaat tcgggaacac gacccggggt gcaccgctca aatccatctt tgagcgccat
       61 ccaccgcatc gccactgctc atgcccctgg taaatcctcg cctcagactg aggccgcggt
      121 tcggctccgc aacccaactc cccctccgcc taaaatgtgt ccaggctctg caaacggcag
      181 cgatccctta tcccatgttt ctgcccctaa tcctgctggc tctctgcttc gccgcgctct
      241 gctggtttct gcgcaacgat ctgaccgagt acgcggcctt caaaaaactc actgacacgc
      301 gcgatcgtca acgacgcatt cgcgtctgga tcctgcgcag ctttctgctg ttctttgccg
      361 gcaccgccgt ttgcctcgcg gtcctccgcc gcctgaaagc gcccttcgtc tttccttccg
      421 agtttctgcc gctcgccgca cgcttccacg ccgccatccc cggaaaccat gcccacgcaa
      481 gccaggtact tctccctttc atgatcgggg caggcggagg cgtcctgatc ggcattctcc
      541 tcgcattgct tttcgcccgc cgcttcagac cggcacgcct cggcgacatc gacgccctca
      601 tgcctcgcaa cagagccgaa accctgtgga tggcgctcat ttccgtcaac gccggggtca
      661 gcgaagaatt gtttttccgt ctgctgctgc cgcttctgct cacccttgcc acacgcagcg
      721 ctctcacttc attcctgatc gccgccgtca tcttcggcct cgtgcatctc tatcagcggc
      781 tgcccggcat cctcgccacc acgctcatcg gcctcgcgct ctccgccgtt tatctaatca
      841 ccggcaatct ctggatcgcc gttctcctcc acatcgtcct cgatctcatt ggagtcgtgg
      901 tgcgtcccag tcttgagcga tggctccgcc ctgcggcacg acgctgaaat cacccttacg
      961 acgcgaattc ctgaaacgcc tcggccaccc ggcacactta cccgccagga ggaaccttcc
     1021 ccatgagaat ttgcatcaag atcggggatc ggacgtggtg cttcaacatt ccgatccttc
     1081 tctacccatt gcatccaccc aagccggacc ccgaaaagga atatggcgaa ctgatctacg
     1141 acgccaccat ccttgcgtcc gtcaacgccg cggccaaaca actttccgat gccggcgtcc
     1201 gcgaagcgct caacaccgga gtcgcgcatg ccgttgctgc cgcgcagaaa aaagccggcg
     1261 ccaacgtcac gctctctctc gagcgctgaa caacagggcc cacgcaagcc ttcgcgcttg
     1321 tgtgggcccc tgttccttac ctcgcttact gcaatccgcc gcttacaacc agcgtctcgc
     1381 ccgtcagcca tcccgactcg tccgacgcca aaaacaccgc gatcggcgca aagtcatccg
     1441 gctttccgat ccttcccagc ggcgtccgct cgatcaatcc cttcatgaag tccgacccaa
     1501 tgaatccggc cgcatgcgtc ccttcggtct ccacgggtcc tggattgatc gagttcactc
     1561 gaatcttctt cggccccagc tcgctggcca gtacgcgcgt caccgtgtcc agcgcaccct
     1621 ttgtcgccga gtacacaacc gtcgccggcg tcgccgactt actgaccacc gaacccacat
     1681 tgatcaccga tccgccctcg cccaggtact ttgccgcttc gcgcgtcgcc agaatcggtc
     1741 ccagcacgtt ggtgtcgaac atgcggtgga agtgctgctc cgaaacgtcg ccgatcggcg
     1801 caaattcgta gacgcctgca ttattcacca gcaca
//
LOCUS       DASZYN010000730         1983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_174908, whole genome shotgun sequence.
ACCESSION   DASZYN010000730 DASZYN010000000
VERSION     DASZYN010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174908"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /gene="folE"
                     /locus_tag="VHE33_18860"
     CDS             complement(<1..593)
                     /gene="folE"
                     /locus_tag="VHE33_18860"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HVT99567.1"
                     /translation="MARASVNGRREKLDARIIGRTGLEDVSTQDIYREIIRRYDADPS
                     RDGLEKTPERIEKALEFLTKGYHADPNEVLHGALFDVAYDEMVIVKDIEMYSLCEHHM
                     LPFFGKVHVAYIPDGKVIGLSKAPRLVEVFARRLQVQERLTRQIAEAIQEAIHPQGVG
                     VVIEARHLCMMMRGVEKQNSSTVTSAMLGVFQKQNTRA"
     gene            complement(597..1022)
                     /locus_tag="VHE33_18865"
     CDS             complement(597..1022)
                     /locus_tag="VHE33_18865"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HVT99568.1"
                     /translation="MRAFLGRRYRLSASHRLFCESFSEAENRNVYGKCANPHGHGHNY
                     TVEVLVGGEIDPVTGMVFDLGELDGFVNREVVEPFDHQNLNTLECFRAQVPTTENLVI
                     EVEKRLRRVLPAKNSVRVCILETSNNSFEYWEEQQGDRV"
     gene            complement(1096..1509)
                     /locus_tag="VHE33_18870"
     CDS             complement(1096..1509)
                     /locus_tag="VHE33_18870"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HVT99569.1"
                     /translation="MILLTRKAEFSAAHYYWNEAWSAEENARVFGKCANRNGHGHNYT
                     LEVTVAGAVDPVTGFVVDLKELKDAMEREVVSVYDHRHLNLEVPEFKAAIPTTENIAV
                     AVWKRLQGKIPGKARLYRVRVYEMPDLFADYFGED"
     gene            complement(1509..>1983)
                     /locus_tag="VHE33_18875"
     CDS             complement(1509..>1983)
                     /locus_tag="VHE33_18875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99570.1"
                     /translation="LVKRRKLGLRFRYAPEAVAARMYRTFGAMVEGWTKNLALLFGNA
                     PMLAGVRLLQLGVLAGIPALMWFFWWLRTVNPAARTGVLGTPLATAALALVWLRALWG
                     LYGRVAKSNFPFRDCVLAPLGIPLYAWLLWRSWFRVTVVKQVVWKGREQKIGSRE"
BASE COUNT          387 a          725 c          475 g          396 t
ORIGIN      
        1 gcccgcgtgt tttgtttctg gaacacgccc agcatcgccg acgtgaccgt cgacgagttc
       61 tgcttctcca ctccgcgcat catcatgcac aggtgccgcg cttcaatcac cacgcccacg
      121 ccctggggat gaattgcctc ctgaatggct tccgcgatct gccgcgtcag ccgctcctgc
      181 acctgcagcc ggcgcgcaaa cacctccacc agacgcggcg ccttgctcag accgatcacc
      241 ttgccgtcgg gaatgtacgc aacgtgcacc ttgccgaaga acggcagcat gtggtgctcg
      301 cacagcgagt acatctcgat gtccttcacg atcaccatct cgtcgtaggc cacgtcgaac
      361 agcgcgccgt gcagaacttc gttcggatcc gcgtgatatc ccttcgtcag aaactccaga
      421 gccttctcga tccgctccgg cgtcttttcg aggccatccc gcgacggatc ggcgtcgtac
      481 cgccggatga tctcgcgata gatatcctgc gtcgaaacgt cttccagacc cgtccgccca
      541 ataattctgg cgtctaactt ctcccgccgc ccgttgaccg atgctcttgc catcgcttac
      601 accctgtccc cttgctgttc ttcccaatac tcaaatgagt tgttcgaggt ctcgaggata
      661 caaactcgca cagaattttt ggccggtaac acacgccgca gccgcttctc cacctcgatg
      721 accaggttct ccgtcgtcgg cacctgtgcg cgaaagcact ccagcgtatt cagattctgg
      781 tgatcgaagg gctccacaac ttcccggttc acgaagccgt ccagctcgcc cagatcgaac
      841 accatgcccg tcaccggatc gatttcccct cccacgagca cttccaccgt gtaattgtgc
      901 ccgtgtccgt gcggattagc gcacttgccg tacacattcc gattctcggc ctcgctgaac
      961 gactcacaaa agagtctgtg cgaagcgctc agccgatatc gccgccccag aaacgctctc
     1021 atgctttgtc atcccgaccg acgcgagcag cacaagcgaa gcggaggggc ctgcttttcc
     1081 gctggaaccg catggctagt cctccccaaa ataatctgca aacaagtccg gcatctcata
     1141 gacgcgcacc cgatacaacc gcgccttgcc cggtatcttc ccctgcagac gcttccacac
     1201 ggctaccgcg atattctctg tggtcggaat cgccgccttg aactccggca cctccagatt
     1261 cagatggcga tgatcgtata cggagaccac ttcgcgctcc atcgcgtcct tcagctcttt
     1321 cagatcgacc acgaacccgg tcaccggatc gacagcgccc gccaccgtca cttcgagcgt
     1381 gtaattgtgc ccgtgtccgt tacgattggc gcacttgcca aacacgcgcg cgttttcttc
     1441 cgcgctccac gcctcgttcc agtagtagtg cgccgccgaa aactccgcct tgcgtgttaa
     1501 caggatcact actctctgct ccctatcttc tgctctcttc ccttccagac tacctgcttg
     1561 acgacggtca cgcgaaacca gcttcgccac agcagccacg catacagcgg aatccccagg
     1621 ggcgccagca cgcagtcacg gaacggaaaa ttcgacttcg ccacgcgccc atacagtccc
     1681 cacagggctc gcagccacac cagggccagc gccgccgtcg ccagcggcgt gcctaaaact
     1741 ccggttcgcg ccgctggatt cacagtccgc agccaccaga agaaccacat cagcgccgga
     1801 attccggcca ggacgcccag ttgcagcagt cgcacgccag cgagcatggg agcattccca
     1861 aacagcagcg ccaggttctt cgtccatcct tccaccatcg cgccgaaggt gcggtacatc
     1921 ctcgccgcca ccgcctccgg cgcataccgg aaccgcagcc ccagtttccg ccgcttcacc
     1981 agg
//
LOCUS       DASZYN010000731         2670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_238704, whole genome shotgun sequence.
ACCESSION   DASZYN010000731 DASZYN010000000
VERSION     DASZYN010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238704"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2547)
                     /locus_tag="VHE33_18880"
     CDS             complement(<1..2547)
                     /locus_tag="VHE33_18880"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HVT99571.1"
                     /translation="MKTKGFQLTETAKGGQIFLLGLILAIGQNGVAQSVYPLRPNDAH
                     AVYLTRSDFEVRGDGVGDDSDALQRAIDRAQETVHHGIVFVPEGRYRLTRTIHVWAGI
                     RLIGYGERRPVFVLAAQTPGFQEGADKDLLWFADERTPEGQPIADASEFTFFSVVSNI
                     DFEIGAGNPAAVAVRFNVAQHSFVAHAKFHLGSARAAIEHAGNQAFDLHVEGGQYGIV
                     TGKTSPAWQFLLMDSSFDGQKVAAISTHEAGMTLVRDSFADVPVAVEIPPGEVEQLYG
                     RDLTMRNVDTVLRLGDTENLRNEITLENVRCEKVARFEASQDEVGSANPPGFVLHPSM
                     SFVEQSFTLGLRIGPDGREQGIAVDQRHKPLPADAASIKSDIPMLPPMRSWVNVRDLG
                     AKGDGGTDDTTVLQQAINAHQALYFPSGFYRLTGSLLLKANTVLIGLSPFTTQFVLAD
                     NDSHFQGDGPAIPLLFVPRDGTSIVTGIGIATGNGNPRAAGVVWSAGAHSMLEDVDFW
                     RGRSEYVKALEPAAPVPLPFAQRPPMQLDAQYPSLWVKDGGGGVLRGIWSHTGTARAG
                     LAIENTTTPGRIYQFSCEHHMWNEVRIDHAANWRIYDLQTEEENPEGQEAVPVTLDAA
                     KNVTFANTYMYRVSRTALPKKDAVVAHNSDGAEFSNVKVFSQTRLAFDNSVFDEGSGV
                     EVRAHHFVSFVPGAALKKSAPLPLPVAFAPGARLERVATGFSNADGLTADGKGKVYFT
                     DAARNAVYRLNPDEKKAEVIATVPQSPMALAWVPAGALVSLNRDTSASEISLDTGKVT
                     TLESVPGPLRPGSTLLLPVGLHSALEKLTLLLEHKGYQYRIGSNTALRSGL"
BASE COUNT          490 a          991 c          671 g          518 t
ORIGIN      
        1 cagcccgctt cgcagtgctg tattgcttcc gattctgtac tggtagccct tgtgctccag
       61 cagcagcgtt agcttttcga gcgcgctatg cagacccacc ggcagcagca gcgtcgatcc
      121 cggccgcaac ggacccggca cactctcgag cgtcgttacc ttccctgtgt ccagcgaaat
      181 ctcgctcgcg ctcgtgtccc ggttcagcga aaccagcgcc ccggctggaa cccatgccag
      241 cgccatcggc gactgcggca ccgttgcaat cacttccgcc ttcttctcgt caggattcag
      301 ccgatacacg gcatttcttg ccgcatcggt gaaataaacc tttcccttcc catctgcggt
      361 cagcccatcg gcgttgctga aacccgtagc cacccgctcc agcctcgccc caggcgcaaa
      421 cgccacaggc aacggcaacg gtgcgctctt tttcagtgcc gctcccggca cgaacgaaac
      481 aaaatgatgc gcgcggacct ccacgccgct cccctcatcg aacacgctgt tatcgaacgc
      541 cagccgcgtc tggctgaaca ccttcacgtt gctgaattcc gcgccgtccg aattatgcgc
      601 caccaccgca tccttcttcg gcagcgccgt ccgcgacacc cgatacatat acgtgttcgc
      661 gaacgtcacg ttcttcgccg cgtccagcgt caccggcacc gcctcctgcc cttccggatt
      721 ctcctcctcc gtctgcaggt cgtagattcg ccagttcgcc gcatgatcga tccgcacttc
      781 attccacatg tggtgctcac acgagaactg gtagatcctg cccggtgtcg tggtgttctc
      841 gatcgccagt ccagcccgcg cagtccccgt gtgcgaccaa atcccccgca gcactccccc
      901 gccaccatcc ttcacccaca aactcggata ctgagcatcg agctgcatcg gcggccgctg
      961 cgcaaacgga agcgggaccg gggctgccgg ctccagtgcc ttcacgtact cgctccgccc
     1021 gcgccagaag tccacatcct cgagcatcga gtgcgctccc gcactccaca ccacgcccgc
     1081 tgctcgcgga tttccattcc cggtcgcgat cccaattccc gtcacgatac ttgttccgtc
     1141 ccgcggcaca aacagcagcg gaatcgccgg cccgtcgccc tgaaaatgcg aatcgttgtc
     1201 ggccagtacg aactgcgtcg tgaaggggct caacccgatc aacaccgtgt tcgccttcaa
     1261 cagaagcgat cccgtcaggc gataaaaccc gctcggaaag tacagcgcct gatgcgcgtt
     1321 aatcgcctgc tgcagcaccg tcgtgtcgtc cgtgcctccg tcgccttttg cacccaggtc
     1381 gcgaacgttg acccaggacc gcataggcgg cagcatcgga atatcgctct tgatcgaagc
     1441 agcgtccgct ggcagcggtt tgtgcctctg atctacggcg atgccctgct cacgtccgtc
     1501 cgggccgatc cgcagtccga gcgtgaagct ctgctcaaca aagctcatcg agggatggag
     1561 aacgaaccca ggaggattcg cggatcccac ctcgtcctgg ctcgcctcga acctggccac
     1621 cttctcacac cgaacattct ccagcgtgat ctcgttccgc agattctccg tgtctcccag
     1681 tcgcaggact gtgtcgacat tccgcatcgt cagatcgcgc ccgtacaact gctccacttc
     1741 gcccggcgga atctccaccg ccaccggcac gtcggcgaaa ctgtcacgca cgagggtcat
     1801 ccccgcctcg tgcgtgctga tcgccgccac tttttgtcca tcgaaactgg aatccatcag
     1861 cagaaactgc cacgccggcg acgtctttcc tgtgacaatc ccatactgcc caccttcgac
     1921 gtgaagatcg aatgcctgat tcccagcatg ctcgatcgcc gctcgcgccg accccagatg
     1981 aaacttcgca tgcgccacga agctgtgctg cgccacgttg aatcgcaccg ctaccgcagc
     2041 cgggttcccc gccccaatct caaaatcgat attcgaaact acgctgaaga acgtgaactc
     2101 gctggcatcg gcgatcggtt ggccttccgg cgttcgctca tcggcgaacc acagcaggtc
     2161 cttgtccgca ccttcctgga atccgggcgt ctgcgccgcc agcacgaata ccggccgccg
     2221 ttcaccgtac ccaatcagcc gaatgcccgc ccaaacatgg atcgtccgcg tcagccggta
     2281 gcgcccctcc ggcacaaaca cgatgccgtg atgcaccgtc tcctgcgcgc ggtcgatcgc
     2341 gcgctgcagc gcatcggaat cgtcgcccac cccgtcgccc cgcacctcaa aatccgagcg
     2401 cgtcaaatag acggcatgcg catcgttggg acggagcgga tagacagatt gcgcgacccc
     2461 gttctggcca atggcaagaa tcagtcccag caggaagatc tggccgccct tcgccgtttc
     2521 cgtcaactga aatcccttcg tcttcacgct cccgatgcta ctccgcgact gcggtttcgg
     2581 aacctcggcc agaccgtgct gatcccttcg cctcgcccgc tcgttaagtg gccccgattc
     2641 cgctgtccac gacgggtccg gccgactctt
//
LOCUS       DASZYN010000732         4614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_951737, whole genome shotgun sequence.
ACCESSION   DASZYN010000732 DASZYN010000000
VERSION     DASZYN010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951737"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /gene="murI"
                     /locus_tag="VHE33_18885"
     CDS             complement(<1..644)
                     /gene="murI"
                     /locus_tag="VHE33_18885"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HVT99572.1"
                     /translation="MKIGVFDSGVGGLTVLRALLRRVPGDYVYLGDTARLPYGAKSRE
                     TVARYAVSSAKFLVEEQGVEFLVIACNTASALALDAIRDAVAVPVLGVVETGANAADA
                     SSRSRDVLVIATAATVASHAYAAACGERKLRAIEKACPLLVPLVEEGWIDHPVTAEVL
                     RIYMAELMDQAREAGLDADTLVLGCTHYPLLRPQIEDAVPETLRVIDSAEVTAD"
     gene            complement(641..1285)
                     /locus_tag="VHE33_18890"
     CDS             complement(641..1285)
                     /locus_tag="VHE33_18890"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HVT99573.1"
                     /translation="MISRFQKFLFFALLLVCVAMAAFLIRLRNRAQDRLAAQAMGAPA
                     EMAETAASAQKIVTLYVPNDLDDALTAVPANLSLPQDRNAQARVILQRLIEAFRAPGS
                     THPIHVTSGINDIYFLPLGAGKDAGANAGQMAVVDLSGSLAAAQPSGIEPETLTLLSM
                     MATLHANLPAVTQVHFLVDGQQRPTLAGHADLTRTYLAGEDNGAQNAESRVQGR"
     gene            complement(1285..2022)
                     /locus_tag="VHE33_18895"
     CDS             complement(1285..2022)
                     /locus_tag="VHE33_18895"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HVT99574.1"
                     /translation="MRWIEVAGTLAIAATAAGVQQTPPAVQHLPQGPAVPAVPAPLNP
                     PGVQPPRFLVVIDAAHGGTDIGARIGEGIQEKDVTLGLAGRLRSTLHARGVDVLITRT
                     ADVNLPLANRAEAANHAQPSACLTIHATATGSGVHLYTSSLTPAATERFLPWGTAQAA
                     YVTQSMKLESEIDTALAHAAIPVTLGKASVAPMDSMAGPEVAVELAPLVGGHVTSPKP
                     LTDVSYQKTVVDAVAAALEQWRSDWKP"
     gene            complement(2009..3343)
                     /locus_tag="VHE33_18900"
     CDS             complement(2009..3343)
                     /locus_tag="VHE33_18900"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HVT99575.1"
                     /translation="MARIYPFRALRYNLAQVAAQDVVTQPYDKITPAMQQAYYQRSPY
                     NLCRIILRMPELFDQAGDLYANAARDFQAWREAGIVEQEGQPCVFAYAQRFAVPGQPG
                     VMTERRGFIALGELYDYTREVVFRHEQTLAKPKSDRLNLLRATRAHFGQIFMLYSDPA
                     HSAEEVLFANGAAGETLPDIEVTDEYGVVHRVWKVTDPARISLVATAMEDKKLIIADG
                     HHRYETALNYKKERGLPGAGEACERPAAGLPQPSCPEAAAMMTFVNMDAEGLVILPTH
                     RVVFGLAGFEPARMLEAVRPYFEIQEMERDRVQAAGYKVPGPGTAFIAVTAKGWFLLT
                     AKAGPVAEALKAVSARQRQLDVVQLHALVLEKLLGISQEAIRDQTHLRYLRDAAEAAD
                     QVVRGEANVAFLMNPVTMEQLREVAFAGEVMPQKSTDFYPKLLSGLAIYALD"
     gene            3578..>4614
                     /locus_tag="VHE33_18905"
     CDS             3578..>4614
                     /locus_tag="VHE33_18905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVT99576.1"
                     /translation="MASVNGRERGGKTLSGPYRVMLKARRIPQWSLLLAPLLLLPLSQ
                     THAQETDNPDNPLDSVHVATPAPVAKKTDPSIPLTPGASLRTGIQFRVDTNLVLIPLT
                     VTDPMDRLVTGLEKENFLILEENHQQAIRTFACDDAPVSIGVIMDLSGSMSNKVVRAR
                     GAVLQFMKTSNPQDEFFVIGFNDRPVLITDYTSSPDDVEARLATVQPGHRTSLLDAIY
                     MGLEKMKQAKYQRKALLIVSDGGDNNSRYTENEVRAAVKEADTQIYSIGIFDPEAATV
                     EERNGPMLLNDISSETGGRLFRVDDLTEMGDIATRISAELRNEYVLGYKTAETRKDGK
                     WRKVKVKLSPPP"
BASE COUNT          855 a         1513 c         1402 g          844 t
ORIGIN      
        1 tgatcggctg tcacctcggc cgaatcgatg actcggagcg tttccggcac tgcgtcttcg
       61 atctggggtc gcagcaacgg atagtgcgtg cacccgagca cgagcgtgtc cgcatcgagg
      121 ccggcctcgc gcgcctgatc catcaactcg gccatgtaga tgcgcagcac ttcggcggtg
      181 acaggatgat cgatccaacc ctcctccacc agcggcacca gcaaaggaca ggctttttca
      241 atggcccgca gctttcgctc gccacacgca gccgcgtagg catgactggc caccgtggcc
      301 gcggtcgcga tcaccagcac gtcgcgcgat ctggaactcg cgtcggcggc gttcgcgccg
      361 gtctccacga caccgaggac gggaaccgcg actgcatcgc gaatcgcatc gagggccagc
      421 gcactggcgg tgttgcaggc gatcacaagg aactccacac cctgttcctc gaccagaaac
      481 ttcgcactcg agaccgcgta gcgcgccacg gtttcccgag acttcgcgcc atagggcaag
      541 cgcgccgtgt cgccgagata cacgtagtcg cccgggacac gccgcaagag cgcccgcagc
      601 accgttagcc cgcccacacc ggaatcgaaa actccaatct tcatcgcccc tgcaccctgc
      661 tttcggcatt ttgcgccccg ttgtcttccc cggccagata ggtccgcgtc agatccgcgt
      721 gtcccgccag ggttggccgc tgctggccat ccaccagaaa atgcacctgg gtgaccgcgg
      781 gcagattggc gtggagcgtc gccatcatcg acagcagcgt cagcgtctcc ggctcaatgc
      841 ctgaaggctg cgcggcagcc agcgacccgc tcaaatcgac gacggccatc tggcccgcat
      901 tcgcgccagc atcttttccg gcgcccaggg gcagaaagta aatgtcgttg atgccggagg
      961 tgacatggat cgggtgcgtc gaacccgggg cacgaaacgc ctcgatcagg cgctgcaaaa
     1021 tgacacgcgc ctgcgcattc ctgtcctggg gcagggacag gttcgccggc acggcagtca
     1081 gagcatcgtc cagatcgttc ggcacgtaga gggtgacgat cttctgggcg ctggcagcgg
     1141 tctcggccat ctcggccggt gcgcccatcg cctgcgcggc cagccggtcc tgcgcgcgat
     1201 tgcggagacg gatcaggaac gccgccatgg ccacgcagac cagcaacaac gcgaagaaga
     1261 gaaatttctg aaaccttgaa atcatcaggg cttccagtcg ctgcgccact gttcgagcgc
     1321 cgccgccacc gcatccacaa cagtcttttg gtaactcacg tcggtcagag gtttcgggga
     1381 agtcacatgg ccgcccacca gcggcgccag ttcgacggcc acttcagggc ccgccatgct
     1441 gtccatgggc gctaccgacg ccttgcccag ggttacggga atcgcggcat gggccagcgc
     1501 cgtatcgatc tccgattcga gtttcatgct ttgcgttacg tacgcagcct gcgccgttcc
     1561 ccacggtaaa aatcgctctg ttgccgccgg ggtcagcgac gatgtataga gatgcacgcc
     1621 gctgccggta gctgtggcat gaatcgtcag gcacgccgaa ggctgcgcgt gattggcggc
     1681 ctcggcgcga ttggctagcg ggagattcac atctgccgtc cgtgtgatga gcacatccac
     1741 gccgcgcgcg tgcagcgtcg aacggagccg ccctgcaagc cccagggtga cgtccttctc
     1801 ctgaatgcct tcgccgatgc gtgctccgat gtcggtaccc ccgtgggctg catcgatcac
     1861 caccagaaaa cgcgggggct gaactccggg cggattgagc ggcgcgggca ctgcgggcac
     1921 cgccggtccc tgcggcaaat gctgcaccgc gggtggagtt tgctggacgc ctgccgccgt
     1981 cgcggcgatc gcaagcgttc cggccacctc aatccagcgc ataaatcgcc agaccgctca
     2041 gcagcttcgg ataaaagtcg gtcgacttct gcggcatcac ttctccggcg aaggccacct
     2101 cgcgcagctg ctccatcgtg accggattca tgaggaaggc cacgttcgcc tcgccccgca
     2161 cgacctgatc tgcggcctcc gcggcgtcgc gcaggtagcg cagatgggtt tggtcgcgga
     2221 tcgcctcctg ggagattccc agcagctttt cgaggaccag cgcgtggagt tgcacgacat
     2281 ccagctgacg ctgccgcgcg gaaaccgcct tcagcgcctc ggcaacgggg ccggcctttg
     2341 ctgtcaacag gaaccatccc ttggcggtca cggcgatgaa agccgttcct gggccaggta
     2401 ctttgtaccc cgcagcctgg acccggtctc tctccatctc ctgaatttcg aaatacggcc
     2461 gtacggcctc cagcatgcgg gccggctcaa acccggcgag tccaaacacc acgcgatggg
     2521 tgggcaggat caccagccct tcggcgtcca tgttcacgaa ggtcatcatg gcggcggcct
     2581 cgggacagga cggttgcggc agccccgcgg cgggccgctc gcaggcttcg ccggctccgg
     2641 gcagaccgcg ctccttctta tagttgagcg ctgtctcgta gcggtgatgc ccgtcggcga
     2701 tgatcagctt tttatcttcc atcgccgttg ccaccaggct gatgcgcgcc ggatcggtca
     2761 ccttccacac ccggtggacg actccatact catcggtgac ttcgatatcc ggcagcgttt
     2821 ccccggccgc gccatttgcg aacagcacct cttccgcact gtgcgccgga tccgagtaca
     2881 acatgaaaat ctgcccgaaa tgcgcccggg ttgcgcgcag caggttgagc cggtcgctct
     2941 tcggctttgc cagggtttgc tcgtggcgga ataccacctc gcgggtgtag tcgtacagct
     3001 cgccgagcgc gataaagcct cggcgctcgg tcatgactcc cggctgaccc ggtacagcga
     3061 accgctgcgc ataggcaaac acgcagggct ggccctcctg ctccacgatc ccggcctctc
     3121 gccatgcctg gaagtcccgc gccgcattcg cgtagaggtc gccggcctgg tcgaacagct
     3181 cgggcatccg caggatgatg cggcacaggt tgtaggggct ccgctggtaa taggcctgct
     3241 gcatcgcggg agtgatcttg tcgtagggtt gggtaacgac gtcctgggca gccacctggg
     3301 cgaggttgta acgcagcgcg cgaaacgggt aaatgcgggc cataatcagg ttctgtttcc
     3361 cgattctatc ctgcgcgtct gctcttgccc tcggcgtcgc gcgggcgtgg gccaaaatcc
     3421 tggcgcgctg catctcgtgt gcctgcctgc gcgcatcgta tgcaataggg catgcggagg
     3481 aaaattcggc aattgtcact tcattgtttc ggccggggat tggctattgc ggaagctgcc
     3541 tcaatcgtgc cagtatcgac acacgagagg taaggccatg gcgtccgtga atgggcgcga
     3601 acgtggtggt aagacattgt cgggacctta tcgggtgatg ttgaaagccc gccgtattcc
     3661 gcagtggtca ctcctgctcg ctcccctcct cctcctgccg ctatcccaga cgcacgccca
     3721 ggaaacggat aacccggata atcctctgga ttcggtgcat gtggcgactc cagcgccggt
     3781 tgcgaaaaag accgatccct cgatcccgct cacgcccggc gcgagcctgc gcaccggcat
     3841 tcagttccgg gtggatacca acctggtcct tattccgctc acggtgaccg atcccatgga
     3901 ccggctcgtc accggtttgg agaaggagaa ctttctcatt ctcgaagaga accatcagca
     3961 ggcgatccgc accttcgcgt gcgacgatgc ccctgtgtcc atcggcgtga tcatggacct
     4021 gagcggcagc atgagcaata aagttgtgcg tgcgcggggc gccgtgctcc agttcatgaa
     4081 gacctcgaac ccgcaggacg aattctttgt catcggcttc aacgaccggc cggtactgat
     4141 caccgactac acttcgtctc ccgatgacgt tgaagcgcgt ctcgccacgg tgcagcccgg
     4201 ccatcgcaca tctctgctgg atgccattta tatgggcctg gaaaaaatga agcaggccaa
     4261 gtatcagcgc aaggctctgc tgatcgtttc cgatggcggc gacaacaaca gccgctacac
     4321 ggaaaatgag gttcgggccg cagtgaaaga agccgacacg cagatctact ccatcggcat
     4381 cttcgatccg gaagccgcta cggtggaaga gcggaatgga ccgatgctgc tgaacgacat
     4441 cagcagcgaa acgggcgggc gcctgttccg cgtcgacgac ctgactgaga tgggcgacat
     4501 cgccacgcgg atcagcgcgg aactgcgcaa cgaatacgtc ctcggctaca agaccgccga
     4561 aacccgcaag gacggcaaat ggcgcaaggt gaaggtcaaa ctttcaccgc cgcc
//
LOCUS       DASZYN010000733         6881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_685846, whole genome shotgun sequence.
ACCESSION   DASZYN010000733 DASZYN010000000
VERSION     DASZYN010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685846"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(297..701)
                     /locus_tag="VHE33_18910"
     CDS             complement(297..701)
                     /locus_tag="VHE33_18910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HVT99577.1"
                     /translation="MESEQFSTSTNPEIPRARRYEIGTAIRFRVRGEREWQEGLTENI
                     SISGLLIRSGRALEPRTAIEMRFFLPVELDGECAAEVFCRGFVIRSSKCRIPAGTYII
                     AAKIMHSRFLRQMSSRDYAAKDAVSGHRRLSV"
     gene            complement(962..1648)
                     /locus_tag="VHE33_18915"
     CDS             complement(962..1648)
                     /locus_tag="VHE33_18915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT99578.1"
                     /translation="MVAITNSLPSPIRILIADDHPLFRDGLRRLLQAEPGFEVVGEAS
                     DGDVLIELTRKTKPDILLLDLAMPRQNGMEVLRELSVSKLPVRTLLLTASIEKAQIVQ
                     ALKLGAYGVITKESTTQRLFDSIHCVMNGQYWVGRESVTDLVRALRSVSPQEKEPRAR
                     DYGLTTRELEIVTLVVAGYSNPDIAQRCSISEQTVKHHVSNVFDKLGVSNRLELALFA
                     VNHRLTAAAS"
     gene            complement(1944..3338)
                     /locus_tag="VHE33_18920"
     CDS             complement(1944..3338)
                     /locus_tag="VHE33_18920"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HVT99579.1"
                     /translation="MHQFAIHRSSESQTAGRAGLRHGRRSGTSDSRVRGGSRSRRFLL
                     YTGFVLGFCALLAFAGCGSIEGNGGLLVVGGGVNFGSVTVGSIGTATVSLMNGGGGAV
                     SVSSVHLSGGPFTIASSATFPVSVAAGGTFSFQVQFAPSAAGEATGTITVLSSVPGSA
                     PTVSLAGLGVSAGGGSGSGSGPGVLSGISCSKASMVGSGTDNCTVTLSGPAASGGLAI
                     SLASNSSSVTVPAGVTVSANATSATFAATVAAVPSAQTASLTAAAGGITKAFSLQLNA
                     ALKVLTANASQVSFGTVALNSTATQSLVLSSTGTQAVTVQSAALTGAGFSLGAVALPV
                     TLNPGQSITLQLGFLPTLAGAASGQITVTSDATSGATMAIPLSGSGGVAYAVNLTWNA
                     PASSPDPVVSYRIYRAVNGAASYQLLNSAANPGTTYTDTTVANGQSYVYVVTSVDAGG
                     MESVPSNTFAATIP"
     gene            3881..4933
                     /locus_tag="VHE33_18925"
     CDS             3881..4933
                     /locus_tag="VHE33_18925"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVT99580.1"
                     /translation="MDRCDVAIAGAGIIGLATALELAAHGRKVTVFDQGEAMSEASRA
                     AAGMLAGADPENPPELNDLARLSLSLYPQFLARVESLSKKSIPIRTHSAIQGLHHAPR
                     DAQSLSASDLLQRAPHVHPNDWQFVLLHETSFDAWDLAEALPDAARAAGIDLREHTQV
                     RSARSTAAGVEIVTDAGTYSAAAFLNATGAWSPALEPALPVMPRKGHMLTVELPGPAQ
                     MDCVLRIPGVYIVPRGNNRYTIGSTVEDAGFDRTVDPLRIHELFNRAADLWPPLREAT
                     IAETWVGLRPGSPDGLPILDQTGENSWVATGHFRNGILLGPGTGRILSQCILGQKPDI
                     DIAPFRLARFVAAPVS"
     gene            5162..6295
                     /locus_tag="VHE33_18930"
     CDS             5162..6295
                     /locus_tag="VHE33_18930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HVT99581.1"
                     /translation="MATATVVKGLEGVVAANSGICWIDGYAGVLAYRGIDIHELADHS
                     TFEETTYLLWHGKLPSRSQLDEFSRKLTEARRIPKEIYDLLRSVPKTATPMEALRTAV
                     SALSFYDPAEAAVDHDSNVRKAFALTAQMAMLVAAYDRIRKGKDIVEPDPSLPHAANF
                     LWMLHGVKPISAAVGAFDMALILHADHELNASTFAARVIAGTMADMHSAITGAIGALK
                     GPLHGGANEEVMKMLFEIGKTGGDPVEYVKTLLSQKKKVFGFGHRVYRTEDPRATHLR
                     KMSEQLSKDSGNTKWFDMSRRIEEFVKDEKKLNANVDFYSASTYTTLGIDLDLFTPIF
                     AVSRIAGWTAHVIEQLDDNRLIRPRAEYTGPDYPVKYVPLNQR"
     gene            6358..6804
                     /locus_tag="VHE33_18935"
     CDS             6358..6804
                     /locus_tag="VHE33_18935"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT99582.1"
                     /translation="MTLPNAPIAREGFFATHFFTVKDQDKSQDFYVRILGGKLIRPKD
                     PCYIKLANTWIILNSGGGPTPDKPEVILETPADLNRVTTFLNLRVADIWACYREWGGK
                     GATFLTEPLPNPDGWEWRCYMRDPDGYIIEVGQYTQLAIDWFNSHS"
BASE COUNT         1345 a         2491 c         1774 g         1271 t
ORIGIN      
        1 ggcaggaaga catcgaaacc cttccccgcc agcacctcgg caacggtctt ttcgtgctgg
       61 tgctgcgtgt aaaccgccca ccaggcgggt gcgtcggcga gatcctcggt gttggctgcg
      121 cccggcatct tgttcgctcc agggtttttg agtacggctc aagcatagct ccgccctgtg
      181 agccacatgt gcccgttttc cacagctgag gtcaaaccct gaaaactgct cgcagccgga
      241 tttgcacccg gatgcccctg tcctctgcaa cttcgtgatt taacaggaaa ttttgatcaa
      301 acagataacc gtcgatgacc actcactgca tctttcgctg cgtaatccct gctgctcatt
      361 tggcgaagaa aacgtgaatg cataatcttg gcagcaatta tgtacgtacc agccgggatc
      421 ctgcatttcg acgatcggat cacgaaccct cggcagaaaa cttccgcggc gcattcgccg
      481 tccagttcaa caggaagaaa aaaccgcatc tcgatcgcgg tcctgggttc cagcgcacgt
      541 ccggagcgga tcagcaaacc tgaaatgctg atgttctccg ttaatccttc ttgccactcc
      601 cgttccccac gtacacggaa ccggatagcg gtgccaattt catagcgccg ggctcggggg
      661 atctccgggt tggtcgaagt actgaactgc tccgactcca tcccctctcc ttgcccactt
      721 tccactgcat ctaagcgcag ccttttcata ccgacgtgcg atgttcaggt tagggcagtt
      781 cccaggggac agcaatggcc cgacagtact atcgtcatat gactgcatag tgctaccaaa
      841 gtggaaccat ggtgtgttac cgaggtgccc acatccagcc gctccactcc agtggtgtgc
      901 ggcaacgcca aagcgtttct ccgggcggag aaatcatgct tttttcaggg aaacttcgcg
      961 cttagctcgc ggccgccgta aggcggtgat tgaccgcaaa aagggccagt tccaggcggt
     1021 tcgatacgcc cagcttgtcg aaaacgttgc tcacgtggtg cttcaccgtc tgctcgctga
     1081 tcgaacaccg ctgcgcaata tccggattcg aatatcccgc caccaccagc gtcacaatct
     1141 ccagctcccg cgtcgtcagc ccgtagtccc gcgctcgcgg ctccttttcc tgcggcgaaa
     1201 cgctccgcag cgcccgcacc aggtccgtga cgctctcccg gcccacccaa tactgtccat
     1261 tcatgacgca gtggatgctg tcgaacaacc gctgcgtcgt cgattccttt gtgatgaccc
     1321 catacgcgcc cagcttcagc gcctggacga tctgcgcctt ctcaatcgac gccgtcagca
     1381 acagcgtgcg caccggcagc ttcgagaccg aaagctcccg cagcacttcc atcccgttct
     1441 gccggggcat cgccagatcc agcagcagaa tatccggctt cgtcttccgg gtcagctcga
     1501 tcagcacatc tccgtccgat gcctcgccca ccacctcaaa accgggttcc gcctgcagca
     1561 gccgccgcag cccatcccgg aacagcgggt gatcgtcggc gatcaggatg cgaattggcg
     1621 aggggaggct attggttatg gcaaccatga cttctgtccc tgcaaggggg atcgttcctc
     1681 caaagcccgc gcctcatgcc tctcagagtt tcttccatgg gcttttcgca gagaatgacc
     1741 ctccgcggac tttgcccacg tgcaggcagg ctgaaatgga gctgagtttc tcggacaggt
     1801 ccttgcggtc gcttgcgcgc atggtcccat gcccattata gcgttcgagc cgggctgagc
     1861 ctgaaacaaa atcgccatcc ccaaaatcct cagcctcgcg ctcgccgccg ggcccacagc
     1921 ctttgtgaca atcgatccgc tcctcacgga atcgtcgctg caaacgtatt cgatggcacg
     1981 ctctccatcc cgcccgcatc gacgctcgtc accacataca cataggactg gccgttcgcc
     2041 accgtcgtat ccgtgtacgt cgttcccgga ttcgccgccg aattcagcag ctggtacgac
     2101 gccgccccgt tcacggcccg atagatgcga taactcacaa ccgggtccgg cgagctcgcc
     2161 ggagcattcc acgtcaggtt gacggcatac gcaacgccgc cgcttccgct cagtggaatg
     2221 gccatcgtcg cgccgctcgt ggcgtcgctc gtgaccgtga tctgcccgct tgccgccccc
     2281 gcaagcgtcg gcaggaatcc cagttgcagc gtgatcgatt gccccgggtt cagcgtcacc
     2341 ggcagcgcca ccgcgccaag actgaaaccg gcgcccgtca gagccgccga ttgaactgtc
     2401 accgcctgcg tgcccgtcga gctcagcacc aacgactgcg tcgccgtgga gttaagcgcc
     2461 accgtgccga acgatacctg agaagcattc gccgtcagga ccttcagcgc agcattcagc
     2521 tgcagggaaa acgccttcgt gatcccgccc gcggccgccg tcaacgacgc cgtctgcgca
     2581 ctcggcaccg ctgccaccgt cgctgcaaag gttgcactgg tcgcgttggc cgaaactgta
     2641 acgccggcag gaaccgtaac cgaactgctg ttgctggcta gactaatggc gagaccgccg
     2701 ctcgctgccg gcccgctcag cgtgaccgtg caattgtccg ttccggaccc caccatcgaa
     2761 gccttgctgc acgaaatccc gctgagcaca cccggccccg atcccgatcc cgacccgccg
     2821 cccgcggaaa ctccgagccc ggcaagactc accgtcggtg cgcttcccgg cacgctgctc
     2881 agaaccgtga tcgtacccgt cgcctctccg gccgcagacg gtgcgaactg cacctggaag
     2941 ctgaacgtcc ccccagccgc aacactcacc ggaaaagtcg ccgagcttgc gatcgtgaag
     3001 ggcccaccgc tcaaatgaac gctcgatacc gaaaccgcgc ccccgccgcc attcatcagc
     3061 gataccgtcg ctgtcccgat cgatccgacc gtcaccgagc cgaagttcac cccgccgcct
     3121 acgacgagca acccaccgtt gccctcgatc gatccgcatc ccgcaaacgc cagcagagcg
     3181 cagaagccca gcacaaatcc cgtgtaaagc agaaaccggc gcgaacggct cccgccgcga
     3241 actctcgaat cgctcgttcc cgaacggcga ccgtgccgca atccggcgcg gcctgccgtc
     3301 tgggattctg acgagcgatg aatggcaaac tggtgcatct acctcacctc gagtcgtacg
     3361 gccattcgtt ctcgcgggcc cggcgcacgc cgagtttgcc tttcccttgg gggtggaaag
     3421 attggatctt cacgggagcc caaacaattt tcgggcccag agcggaactc ccgcggttcg
     3481 atcctgctca gaacatttgg ccagtctgcg agattctagg cggtgccgaa gcacgtggat
     3541 atccaaccaa ggcaacagtc actgttacct tcgtgctatg cgcgcatcct cgcgcacaga
     3601 gcgatgcacg ctgctcccct gtccccaatt tccacattgc gcgaaatctc ctcgcctccg
     3661 cgagtctgga aaaaacgcac acatccgcga acattcttcc cttcctgcgc tgcatcgcgc
     3721 acgatgcaac gcagatccgc atcaacaaaa tctccagcaa cgatgcgcct ctgcgccctg
     3781 acgaaagtta catcctcccg taccgcggtg ccgcgaagtt ttgccgcctt catcgcgctc
     3841 ctcttcacgc gcatcgttca tcgcaaatta aaatcgattc atggaccgtt gcgatgtcgc
     3901 gatcgcgggc gcaggcatca tcggactcgc caccgcactc gaactcgctg cacatggccg
     3961 taaggtaacc gtcttcgatc agggagaagc catgtccgag gcctcccgcg ccgccgcagg
     4021 catgctcgcc ggcgccgatc ccgagaatcc tcccgaactg aacgatctcg cccggctcag
     4081 cctctcgctc tatccccagt tcctggcgcg cgtcgagtca ctctcgaaga agtccattcc
     4141 catccgcacg cacagcgcca tccagggcct tcaccacgcg ccccgtgatg cccaatcgct
     4201 ctccgcgtcc gatctcctcc agcgcgcccc gcacgttcac cccaacgact ggcaatttgt
     4261 cctgctccat gagaccagtt tcgatgcgtg ggatctcgcc gaagccctcc ccgatgccgc
     4321 ccgcgctgcc ggcatcgacc tgcgcgagca tacccaggtc cgcagcgccc gctccaccgc
     4381 tgccggcgtc gaaattgtaa ccgatgccgg cacatattca gccgccgcat tcctgaacgc
     4441 caccggcgcc tggtcccccg cactcgagcc cgctctgccc gtgatgccgc ggaagggaca
     4501 catgctcacc gtcgagcttc ccggccccgc ccagatggac tgcgtcctcc gcatccccgg
     4561 cgtctacatc gttccccgcg gcaacaaccg ttacaccatc ggctcgaccg tcgaggacgc
     4621 cggtttcgac cgcaccgtcg acccgctgcg cattcatgag ttgttcaacc gcgccgccga
     4681 tctctggccg cccctgcgcg aggccaccat cgcggaaacc tgggttggcc tccgccccgg
     4741 ctcgcccgat ggtctgccca tcctcgatca gaccggcgaa aactcgtggg tcgccaccgg
     4801 ccacttcagg aacggcatcc tcctcggacc cggcaccggc cgcatcctca gccagtgcat
     4861 cctcggccag aaacccgaca tcgacatcgc tccctttcgt ctcgcgcgat ttgtcgccgc
     4921 gccggtctcc tagtgagcgc gtctcttttc cgctccccgc cgtcatcctt cgtatcgaat
     4981 gaatcggccc cggctccggc aaacgcggcg aacgacacgg attttcgcgt aatctaggaa
     5041 tagaatcttc gacggtgaaa cgtctcccag cagcggaaat ccggccgggc ttgcatcgcg
     5101 aaacagctcg acagcatcct ggtcattgaa ccggtttccg gggagaggga aagggcagca
     5161 catggcgacg gcaacagtag tcaagggtct tgaaggagtc gtagcggcga attccggcat
     5221 ctgctggatc gacggctacg ccggagtgct cgcgtatcgc ggcatcgata tccacgaact
     5281 ggcggatcac tccaccttcg aggaaaccac gtacctgctc tggcacggca aactccccag
     5341 ccgctcccag ctcgacgagt tctcccgcaa gctcaccgaa gcccgccgca tccccaaaga
     5401 gatttacgac ctgctccgct ccgtgccgaa aaccgcgacc cccatggaag ccctgcgcac
     5461 cgccgtctcc gcgctcagct tctacgatcc ggccgaagcc gctgtcgacc acgactccaa
     5521 cgtccgcaag gccttcgccc tcaccgcgca gatggccatg ctggtcgccg cctacgatcg
     5581 catccgcaag ggcaaggaca tcgtcgagcc cgatccttcc ctgccccacg ccgccaattt
     5641 cctctggatg ctccacggcg tcaaacccat ttccgccgcg gtcggcgcct tcgacatggc
     5701 tctgatcctc cacgccgatc acgagctgaa cgcctctact ttcgccgcgc gcgtcatcgc
     5761 cggcaccatg gccgacatgc attccgccat caccggcgcc atcggcgcgc tcaaaggccc
     5821 cctgcacggc ggcgccaatg aagaagtgat gaagatgctc ttcgaaatcg gcaaaaccgg
     5881 cggcgatccg gtcgagtacg tcaaaacgct cctgtcgcag aagaaaaaag tcttcggctt
     5941 cggccaccgc gtctatcgca ccgaagatcc ccgcgccacc cacctgcgca agatgtccga
     6001 gcagctcagc aaggattccg gcaacacgaa gtggttcgac atgtcccgcc gcatcgagga
     6061 gttcgtaaag gacgagaaga agctcaacgc caacgtcgac ttctactccg cctcgaccta
     6121 caccaccctc ggcatcgacc tcgatctctt cacgcccatc ttcgccgtca gccgcatcgc
     6181 cggatggacc gcccacgtca tcgagcagct tgacgataac cgcctcatcc gcccgcgcgc
     6241 cgaatacacc ggccctgact acccggtcaa gtacgtgccc ctcaaccagc gctagcgccg
     6301 ctccaatgtg cggcgcataa ccatcctcgt atcctgattc gaaacgggag gcgccgcatg
     6361 accctgccga acgctccgat cgcccgcgaa ggcttcttcg ccacacactt cttcaccgta
     6421 aaggaccagg acaagtccca ggacttctac gtccggatcc tcggcggcaa actgatcagg
     6481 ccaaaagatc cctgctacat caagctcgcc aacacgtgga tcatcctgaa ctccggcggc
     6541 ggcccgaccc ccgacaagcc cgaggtcatt cttgaaaccc ccgccgatct gaaccgagtc
     6601 actactttcc tcaatttgcg agtggccgac atctgggcct gctaccgcga atggggcggc
     6661 aagggcgcga cttttctcac cgagcctttg cccaatcccg acggctggga gtggcgctgc
     6721 tatatgcgtg accccgacgg ctacatcatc gaagtcggtc aatacacgca actcgccatc
     6781 gactggttca acagccacag ttaatccgcc aattgggccg ctgccgcagt ttcccaatcg
     6841 ccggtacgtc ggcaatgacg ccatcctgac ctgcacttca a
//
LOCUS       DASZYN010000734         7198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1072573, whole genome shotgun sequence.
ACCESSION   DASZYN010000734 DASZYN010000000
VERSION     DASZYN010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072573"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..594
                     /locus_tag="VHE33_18940"
     CDS             112..594
                     /locus_tag="VHE33_18940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99583.1"
                     /translation="MLGDEAEALNLVENTVASVDIDPCADPVTAQGLVQERVLDGALE
                     IMQRHDPASFAAAPATAQGGCIEDETVPLSGAQVAELMAGTGRGPLREWLARLTQAQR
                     AVFIQRVVLGKSNADTAQAINRHARPSIWTAEAVSSIFRQALCSLASSLVHSASVAVV
                     "
     gene            899..2287
                     /locus_tag="VHE33_18945"
     CDS             899..2287
                     /locus_tag="VHE33_18945"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HVT99584.1"
                     /translation="MAAAQGPPAGGSGSAPAGPILIPQPPVDSSTYQSSVSDGKVTPG
                     VLPLSLSDAIQRGLKFNLGMILTSQNTLSARGARLEQLQALLPTVSGSVKEAVMETDL
                     QAQGLRIPGFPAIIGPFGYTDIRGSLSWSLVNLSSLQNYLAQRHNFKAAQLSAQDARD
                     LVVLTVGNSYLTVIADAALVQSAQAEVDTSKVSLDQAQGNHEAGTAPLLDELRAKVDY
                     QTQQQTLIAAQNAYQKDKIALARAIGLPLEQQFELTDTVPYARFDQLDPDKTVQQALA
                     NRSDLKALEEQVAAAEHARKAATAERFPTITFNGDYGDIGINPSHSHGTGDAVGSLDV
                     PIFEEAKLRGDAKQAQSQLEQMRARLSDMRGQISADVRDSILDIQAAEKLVEVAKSNV
                     DLAKEALSESQQRYKAGVSDNLAVSQAQQAVAQADEQYVASLYRHNVAKLSLARALGV
                     AQSNYKDYVGGK"
     gene            2288..3511
                     /locus_tag="VHE33_18950"
     CDS             2288..3511
                     /locus_tag="VHE33_18950"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HVT99585.1"
                     /translation="MAEQNTEQSRPTEERPENTSARPRRRFIIIGVVAVLVVGALLFW
                     WHSTYYEDTDDASVDGHLVQISSRISGHVAKVLVQQNQYVDAGTLMVEIDPSDYQTAV
                     NQAEANLEAAVANYEASRVNVPVININAGSNLNNANADVQGATAGVAQAEKQLQAAEA
                     RVTQAQANYTKTKLDLERYTPLVQKDIISKQQYDAAVAAAAAGQASVAEAEASQIAAQ
                     DAVRIARQRVNQAQSSLKYAQTGPQQVAIQKAKADQAAGQVDQAKASLAQAKLNLSYC
                     EIHAPVSGIITTKSVEVGQNIAAGQALMTLVSLDDVWVTANFKETQLQHMQVGQAVTI
                     HVDAFGREFDGKVTQIGGATGSMLSLFPPENATGNYVKVVQRIPVRIDLTKPDENKDH
                     MLRPGMSVEPKVRVK"
     gene            complement(3592..4407)
                     /locus_tag="VHE33_18955"
     CDS             complement(3592..4407)
                     /locus_tag="VHE33_18955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99586.1"
                     /translation="MKGKSLSLLALLLAAPLSSYAAEQLIPAGSLISCTLSEPKLSSK
                     NTAIGDPVLCQLGHAERYGRSVLPYNSYLVGRFEDFKDPGHLVGKGWMELRFDRMVIE
                     PNTIIPIDAKVVDVPGYGVDRYGRILGKGHPVRDTVTWMIPVLWPIDLINLPRRGPRP
                     TLKEETRLTLKVMDDLGIPMMDQPPLDQTPSGLYKRPSAYEQPDPPPPPPQQITYSQP
                     APPPPPQQITYVQQAPPPTYVYMAPPPVVAYVPPPRTVVVYRNGYAYRVAVPY"
     gene            complement(4691..7132)
                     /locus_tag="VHE33_18960"
     CDS             complement(4691..7132)
                     /locus_tag="VHE33_18960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT99587.1"
                     /translation="MGNLLQDLRYTLRTLLRAPGFSSVVILSIALALAANATVFSVAN
                     GLLWGVLPVRDPGRVVMFSEGNSFSYPDYLDYRDQTRATFENGIAAHFPLIPASIGGK
                     GEPERVWGQSVSGNFFPMLDVPMALGRSILPEDDTAARTHVVVLSSGLWRRRFAGDPN
                     ILNHDVALNGQQYTVVGVAPPGFYGVDRGIVSEFWVPLAVSEEILPDLKTEGNATDKR
                     DDQWLMLDARLKPGVTRAHAAAVVNTVKKRLDDTWHKNDKTHEKITLQNAGGLIAGSA
                     TPVFTLMAVLMIVVGLVLLVACANVANLLLARATGRQKEIAIRLAVGAGRRQLVRQLL
                     IESFLLALAGASAGFLLAAVAARAISSFQLPLPFPIAFDFNVDWRVALFTLGLSVVTT
                     LVFGLVPALRASRPDLVGSLKDGPTGFGRTARSRMRNTLVIVQVSLSLVLLTTAGLFL
                     RSLGNASSIDIGFKSANLLIMSMDPKIQNYSPDKTAHFLSQLHDRVAALPGVRSVSFV
                     GVVPLSIGANMNNFAVDATAGHPAQNGIAFVNTIGAGYFQTMGSPLLRGREFIAQIDG
                     PSAAIINETMAAHLFPGQDPVGRTIRQDKTTYTIVGVARNSKTRTIGEKPTDAVFLYL
                     NAAPQKANSFFGTTILVKTSIDPRALAASVRQQINALDPNMAVFNTETMQEHVDKSLL
                     LPRISSLLLGIFGVIGLTLAAIGLYGVMSYSVRRRTREIGIRMALGAKRPAVLRMILR
                     QGLILTAIGLSIGLALALVLGRFTASVLYGTSGSDPLTFLTVSLVLIASAIAAILVPA
                     LRAAHVEPTTALRCE"
BASE COUNT         1490 a         1911 c         2573 g         1224 t
ORIGIN      
        1 ggtcaggaag cctggcggag gagaatcgtg gctggatccc catgttctgg cagtgacgcg
       61 gcagtcaggg cggagagcgc ggcagtggag atgtttcaac tggctgcgct gatgctgggc
      121 gatgaggctg aagccctgaa cctggtcgag aatacggtcg cttcggtcga tattgatccc
      181 tgtgccgatc cggtgaccgc acaaggtttg gtgcaggagc gggtgttgga cggggcgctg
      241 gagatcatgc agcggcacga cccggcctcg tttgctgccg cgccggcaac ggcgcagggg
      301 ggctgcatcg aagatgaaac cgttcccctg tcgggagccc aggttgccga actgatggct
      361 gggactggtc gcggcccgct gcgcgaatgg ctggcaagac tgacacaggc gcagcgcgct
      421 gtgttcattc agcgagtggt gctcgggaaa agcaatgccg atacagcaca ggccatcaac
      481 cggcacgcga ggccttccat ttggacagcc gaggcggtga gcagcatatt ccggcaggct
      541 ttgtgctcgc tggcgtcatc tctggtgcac tcggcgtccg tcgcggtggt ttaaccgaag
      601 acgacctgaa ggttaaagca ggatcggctc cgcacccggg cgaacggtgg tgtcccttct
      661 tcgccgattg ttcgagacgc gacaggggcg cttcgactac tcttacatca gagatttctt
      721 ctgaactttt tattcagcgt agtgcatcca aagcacatac cctgacccgg gacccaggct
      781 gtccgcggtc ccaacagaga ggcttttgct tgtggcggag gcgttagcga ccgatacacg
      841 atgtttgtgc ccaaagcgac ttgccgcatt ggcagcagca ttggcactcc tgagcggcat
      901 ggccgcagct cagggccccc cagcaggcgg atcggggtcc gctccggccg gaccgatttt
      961 gatccctcag ccaccggtgg acagctcgac ctatcagagc agcgtcagcg acggaaaggt
     1021 cactccgggg gtattgcccc tgtcgctaag tgacgcaatc cagaggggac tgaaattcaa
     1081 tctgggtatg atcctgactt cgcagaacac tctgtcggcg cgtggcgcgc gactggagca
     1141 gttgcaggcc ctgctgccaa ccgtgtcggg ctcggtcaaa gaggcagtga tggagacaga
     1201 cctgcaggcg caggggctgc gcattcctgg gtttcccgcg attattgggc cgttcggcta
     1261 tacggatatt cggggatcgc tgagctggtc gctggtgaac ttgtcctcgc tgcagaacta
     1321 cctcgcgcaa cggcacaatt tcaaagccgc gcagctgtcc gcgcaggacg ctcgggatct
     1381 ggtggtgctg accgtcggaa actcctatct gacagtgatt gccgatgcgg ctctggtgca
     1441 gtcggcgcag gccgaagtcg acacctcgaa ggtttcgctg gatcaggcgc agggcaatca
     1501 cgaagccggc acagccccac tgctggatga gctgcgagcg aaggtggatt accagacgca
     1561 gcagcaaacg ctgattgcag cgcagaacgc gtatcaaaag gacaagatcg cactggcgcg
     1621 agcaatcggg ctgccgctgg aacagcaatt cgagctgacg gacacggttc cgtatgcgcg
     1681 cttcgatcag ctcgatcccg acaagaccgt gcagcaggcg ctcgcgaatc gttccgatct
     1741 gaaagctctg gaagagcagg tcgcggcggc ggaacacgcg cgcaaggcgg cgactgcgga
     1801 gcggttcccg accattacgt tcaatggcga ctacggcgat atcggcatca atccgagcca
     1861 ctcgcacgga accggcgatg cggtcggttc gctggatgtg ccgatcttcg aagaagcaaa
     1921 gctgcgcggt gacgcgaagc aggcgcagtc gcagctggaa cagatgcggg cgcggctgag
     1981 cgatatgcgc ggacaaatca gcgctgacgt ccgagacagc atcctcgaca tacaggcggc
     2041 cgagaaactg gtggaagtgg cgaagagcaa tgtcgatctg gcgaaagaag ctttgagcga
     2101 gtcgcagcag cggtataagg cgggcgtctc cgacaatctg gctgtatccc aggcacagca
     2161 ggctgtcgct caggccgatg agcagtacgt ggccagtttg tatcggcaca atgtggcaaa
     2221 actgtcgctg gcccgcgcgc tgggcgtggc gcaaagtaat tacaaggatt acgtgggagg
     2281 aaagtaagtg gcggagcaga ataccgagca gtcgagacca actgaggagc ggccggaaaa
     2341 cacatcggcg cgcccacgcc ggcgtttcat catcattggc gtcgtggcgg tgctggtggt
     2401 cggcgcgctg ctgttctggt ggcactccac gtattacgaa gacacggacg acgcgtcggt
     2461 ggacggccat ctggtgcaaa tcagctcccg catcagcggg catgtcgcga aggtgctggt
     2521 gcagcagaac cagtatgtcg acgccggcac gctgatggtc gagatcgacc ccagcgacta
     2581 ccagacggca gtgaaccagg cggaggcgaa cctcgaggcg gccgtcgcta actacgaggc
     2641 ttcgcgcgtc aacgtcccgg tgatcaacat caacgcaggc agcaatctga acaacgccaa
     2701 cgccgatgtc cagggcgcaa ccgccggcgt agcgcaggcg gagaagcagc tgcaggcagc
     2761 ggaggcgcgc gtaacccagg cgcaggcgaa ttacaccaag acgaaactgg atctggagcg
     2821 ttacacgccg ctggtgcaga aagacatcat ttcgaagcag caatacgacg cagccgtagc
     2881 ggctgccgcg gcaggccagg cgtcagtcgc ggaagcggaa gccagccaga tcgccgcaca
     2941 ggacgcggta cggattgcgc ggcagcgtgt gaaccaggcg cagtcatcgc tgaaatacgc
     3001 gcagaccggg ccgcagcagg tagcgattca gaaggcgaag gccgatcagg cagcgggcca
     3061 ggtggatcag gcgaaagcaa gcctggcgca ggccaagctg aacctgagct actgcgagat
     3121 tcacgcgccg gtctcgggga tcatcacgac caagagcgtt gaggtgggcc agaacattgc
     3181 ggccggccag gcgctgatga cgctggtttc gctcgacgat gtgtgggtga cggcgaactt
     3241 caaagagacc cagttgcagc acatgcaggt gggacaggcg gtcacgatcc atgtggatgc
     3301 gtttgggcgt gaattcgacg gcaaggtcac tcagatcggc ggtgcaaccg gatcgatgct
     3361 gagtctgttc ccgccggaga acgcaaccgg caactacgtg aaggtcgtgc agcggattcc
     3421 ggtgcgcatc gacctgacca aaccggacga gaacaaggat cacatgttgc gtccgggcat
     3481 gtccgtggaa ccgaaggtga gagtgaagta ggacaacagc gtatagcgtt tatcaaaggg
     3541 cgtccggtca gccggacgcc cttgttgttt ttgcgatgga tcgaagtgcg gtcagtacgg
     3601 aacagccacg cgatacgcgt agccgttgcg gtacacaacg acggtccgtg gaggcggcac
     3661 ataggccacg accggcggcg gcgccatgta gacataggtc ggtggcggcg cctgctggac
     3721 gtaggtgatt tgttgtggtg gaggcggagg cgcaggctga gaataagtga tctgctgcgg
     3781 tggaggcggc ggtgggtccg gctgttcgta ggcgctggga cgtttgtaaa gcccggaagg
     3841 cgtctgatcc agcgggggct ggtccatcat ggggatgccg agatcgtcca tgaccttgag
     3901 ggtgaggcgc gtttcttctt tcagcgtggg ccgcggaccg cggcgcggca ggttgatgag
     3961 gtcgatgggc cagagaaccg ggatcatcca cgtgaccgta tcgcgcacgg gatggccttt
     4021 gccgaggatg cggccgtagc gatcgacgcc gtagccgggg acatcgacga ctttggcatc
     4081 gatagggatg atggtattgg gctcgatgac catgcggtcg aagcggagtt ccatccagcc
     4141 cttgccgacg agatggccag gatccttgaa gtcctcgaag cgcccgacga ggtagctgtt
     4201 gtagggcagc acggagcggc cgtagcgctc cgcatggccg agctggcaga gcacgggatc
     4261 gccgatcgcc gtgtttttgg agctgagttt gggctctgac agcgtgcagg agatcagaga
     4321 accggctgga atcaactgct cggcagcgta gctcgaaagc ggcgcggcaa ggagcagagc
     4381 aagaagagaa agagatttgc ccttcatgag gaaggcctcc tggctgggtc agctgcatcg
     4441 caggaacacg atggctcacc cggtcatggc gcgagaatcg gaccggcgcc gatgcgcgtt
     4501 gagtgcgttg ggagtcctcg gaatcctcgg gaaggaacag cagggccacc tctgcgtgca
     4561 gggtggctgg gcaaccatga tggaggcagc ggggaggctg tgtcaagaga aatgaacccc
     4621 tcagttcccg agttgcgagg tcggggacat tcagcctgta tcgaacaacc gaagcgcgac
     4681 tgattccggg ctattcgcag cggagggcag tggtgggttc gacgtgggct gcgcgcaggg
     4741 cggggaccag gatggcggcg atggcgctgg cgatcagcac cagcgacacg gtgaggaagg
     4801 tgagcggatc actcccgctg gtgccataga gcacgctggc ggtaaagcgt ccgaggacaa
     4861 gggcgagggc aagcccgatc gacagcccga tggcggtcag gatgaggccc tggcgaagga
     4921 tcatgcggag gacggcgggg cgttttgcgc cgagcgccat gcggatcccg atttcgcgcg
     4981 tgcggcggcg caccgaatag ctcataacac cgtagaggcc gatggccgca agtgtaaggc
     5041 cgatgactcc aaaaatcccg agcagaagcg atgaaatgcg cgggagaagc agcgacttat
     5101 cgacgtgctc ctgcatggtc tcggtattga agaccgccat gttgggatcg agagcgttga
     5161 tctgctgacg cacggaggca gcaagagcac ggggatcgat cgacgtcttg acgaggatgg
     5221 tggttccgaa gaagctgttg gctttttgcg gcgcggcatt cagatagaga aacacggcat
     5281 cggttggttt ctcgccgatg gtccgggttt tcgagttacg ggcaacgccg acgatggtgt
     5341 aagtggtttt gtcctggcgg atggtgcggc cgacagggtc ctggccggga aacagatggg
     5401 cggccatcgt ttcgttgatg atggcggcgg aagggccgtc gatctgcgcg atgaactcgc
     5461 gtccgcgcag cagtgggctg cccatggtct ggaaatatcc ggcgccgatg gtgttcacga
     5521 aggcgatgcc gttttgtgcg gggtggccgg cggtggcatc gacggcaaag ttattcatgt
     5581 tggcgccgat actcaacggc acaacgccga cgaagctgac ggagcgcacg ccaggaaggg
     5641 cggcgacgcg gtcgtggagt tgcgacagga agtgcgcggt tttgtcgggg gagtagttct
     5701 ggattttggg gtccatggac atgatgagca gattggcgga cttaaagccg atgtcgatgg
     5761 aggaggcatt gccgaggctg cggagaaaga gtccggcggt ggtgaggagc acgagggaca
     5821 gcgacacctg gacgatgacg agggtgttgc gcatgcgcga tcgcgctgtg cgtccgaagc
     5881 cggtggggcc gtctttgagc gacccaacca ggtcaggtcg cgaggcgcgc aacgcgggaa
     5941 cgagtccgaa gacgagggtg gtaacgacgg acaggccgag ggtgaacagg gctacacgcc
     6001 agtcgacgtt gaagtcgaag gcgatgggga acggcagagg gagctgaaag ctcgagatgg
     6061 cgcgagcggc cacagcagcg agcagaaatc cggcgctggc gccggccaga gcaaggagaa
     6121 aactctcgat gagcaattgg cgcacgagtt gacggcggcc ggcgccgacc gcgagacgga
     6181 tggcaatctc cttctggcgg ccggtggcgc gggccagcag caggttggcg acgttggcgc
     6241 aggccaccag cagcaccagg ccgacaacga tcatgaggac ggccatgagg gtgaataccg
     6301 gagtggccga accggcaatg agtccgccgg cgttttgcag cgtgatcttt tcgtgggtct
     6361 tgtcgttctt gtgccaggta tcgtcgaggc gctttttgac ggtgttgacg acggccgcag
     6421 cgtgggcacg ggtgactccg ggcttgaggc gagcatcgag catcagccac tggtcgtcgc
     6481 gtttgtcggt ggcgttgcct tcggttttca ggtcgggcag gatctcttcc gatacagcaa
     6541 ggggcaccca aaattcggag acaatgccgc gatcgacgcc gtaaaaacct ggcggcgcga
     6601 caccgacgac ggtgtactgc tgtccgttga gggcgacgtc atgattgagg atgttcgggt
     6661 cgccggcgaa gcgacggcgc cataaaccac tgctgagcac cacgacgtgg gtgcgcgcag
     6721 cggtgtcgtc ttccgggagg atggaacggc cgagcgccat gggcacgtcg agcatgggga
     6781 agaagttgcc gctgacggac tggccccaga cgcgctcggg ctcgcccttg ccgccgatgc
     6841 tggcggggat aagcggaaag tgggcggcga taccgttttc gaaggtggcc ctggtctgat
     6901 cgcgataatc gaggtagtcg ggatacgaga aggagttgcc ttcggaaaac atgacgacgc
     6961 ggccagggtc gcgaacaggc aggacacccc agagcaggcc gttggccacg ctgaagacgg
     7021 tggcgttggc cgccagggca agggcgatgg agagaatcac gaccgacgag aaaccgggag
     7081 ccctgagcag cgtgcggagg gtatagcgaa gatcctgaag caggttcccc atgggtggct
     7141 cctcggtgga agagattggt ggcggaggag agtacgcagg ggatgggggg aaagttcg
//
LOCUS       DASZYN010000735         5201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_496324, whole genome shotgun sequence.
ACCESSION   DASZYN010000735 DASZYN010000000
VERSION     DASZYN010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496324"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..3788
                     /locus_tag="VHE33_18965"
     CDS             144..3788
                     /locus_tag="VHE33_18965"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVT99588.1"
                     /translation="MTFQKLGQLTLLTLGLLASPLFLLAQGTDLGTIRGTVTDPSGAQ
                     IAHAHIEITDVNTQITRKYESGDRGSFDASALPSGKYKVAISAPGFGTSIVENVTVSG
                     SNVATADVVLHPSTVEQSVQVTSDALTINTEDPTLNENLTSTAIIELPRDSRDVYSFM
                     YINPNVTQSGVSGDFKFLGAQSYGAEFSVDGQRANGGIFGSQTQSKPSLESVGEFNVL
                     SNGFSAEYAGIASVRVSTKRGGAAYHGSAFYNNKNSALAAWSLADKNAAASFAPSSFQ
                     PHYPNPYFNITDVGGSVGGPVPHLPKTFFFTAFEHDWTVEPTTTGRVNNLPHPALIAG
                     DFSGVNDSAKPDVPAGTVLTPSEIANDTVDGLGQKFITIPSRLLNPFVQKLFNIYYPH
                     VGLSAPEDPATGRVIGYQTSIPGHSGANLGDLRIDHDFSDSNRLYGVYHGGSENDALN
                     EVAAPLTGLGLSQTDRLNKTLSLSYTHVFGPQLINEARGGFNRQYLFTHSNTTLRGFL
                     KSIGFSDADIAAYGSTVGARELDTHGQAAINIANFQGVGNGGRNTERPLSQNLITFGD
                     TVTWNPGRHNIRLGADFVRNSAEDGFTSGRGQPRGLITYTGSNTSALANLLLGNAPNT
                     VSFTPKGRPAMDVYNWETGIFFQDDFRMNDRLTFNVGLRYDLFTPFTETNDLMVNFDP
                     NLTNPVTGEPGTYIMPSSKTLAYVDPSVIAFGYVTAHSAGVGRSLVHTDWTDVNPRLG
                     FAYRIGGKSVFRGGWGFFTPTSTAHVIRDPLATNTFNQTYTLRSTSGGPPINPWPTQG
                     ETTGTSPNTGGTASGFGNTPTANNVPLDLKNPRLQQWNATFEQQLPSAWVFRVSYIGA
                     YISGEITGRDLDMIAPSDQLFGVTTGDGVTICDPANEGNCAYSDADKARLRFPALGDY
                     ITAFGNNGHGLTASGQAQIQHQTRHLTLSVAYTYLNQKSTGVDSDNDSLGGDAYNQLD
                     PNSDYGTDSFVSHHRVVAYGVLDLPFGRGQRFGDGMPKALDAIVGGWQTTFNMFAKTG
                     TGFTPFYYCNDCDPVEPGNIASGAIDAVGDFGGGFRAKIIGNPNHGAGKDLQWNADAF
                     DLPDTGATLFSNPLNAKRNSLTGPGTYGVNLGIHKTFNLTDRVGIQIGADADNVFNHP
                     LLSPDSDSSDDFANVGSFTLHLDPNTGAILPLATEYYPNPDFGLKYQSVTQEGIDNRR
                     SIRLRGRITF"
     gene            4167..>5201
                     /locus_tag="VHE33_18970"
     CDS             4167..>5201
                     /locus_tag="VHE33_18970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99589.1"
                     /translation="MKNTTIQQNEEARLGPLSQLMELSQVEQKARGLLFTPREIAQQP
                     DTWKTTLAIFRQHQAEVSSFLEHAGVLAPLEERPVVMLVGAGTSDYIGRALALLLREK
                     WGCEVSACASTELLTNLSSYIVSGRKYLWISFSRSGDSPEAVAILKQAIEQYPEISHL
                     ILTCNAHAQMIEVASAALHSYVVVLDDAVNDRSLAMTSSFTNMLIMGQCLAHAWSIEE
                     YAPILEQLSDAGHDFLPKASREAAATAALLLPRACFVGSGTLAGVAKESALKVTEMTA
                     GQVKSISETVLGLRHGPMAALDTETLFVCFVSSDRRRQQYASDLLQEISQKGITGRRI
                     VVGPGSAQHLF"
BASE COUNT         1107 a         1516 c         1469 g         1109 t
ORIGIN      
        1 gagcgggtgg ggcgatgaaa cggcgaatca tggccgtttg gagctatttt tgttccgtat
       61 tgctgctttc tgtatttttt aattgcgtat taatccccgg tagtgctatt tattcgttca
      121 cgcttcattc acggagacca cgtgtgacct tccaaaaact tggacaactg acgttgctga
      181 cgctggggct cctcgcctct cccctgttcc tgctcgccca gggtaccgat ttgggcacga
      241 tccgcggaac tgtaaccgat ccgagtggcg cgcagatcgc ccacgcgcat atcgagatca
      301 ctgacgtgaa cacccagatc acgcgcaagt atgagagcgg agatcgcgga agcttcgacg
      361 cctcggcgct tccttccggg aagtacaagg ttgccatttc ggctcccggg ttcggtacgt
      421 cgatcgtgga gaacgtgacg gtctcgggct cgaacgtagc tacagccgac gtggtgctgc
      481 atccttcaac cgtcgagcag tcggttcagg tcacttcaga cgcgctcacc atcaacactg
      541 aagatcccac gctcaatgag aacctgacgt cgacggcgat cattgagtta ccgcgggatt
      601 cccgcgacgt ttactcgttc atgtacatca accccaacgt gacgcagagc ggggtttcgg
      661 gagatttcaa gttccttggt gcgcagagct acggggctga attctcggtt gacggacagc
      721 gggcgaacgg cggcatcttc ggttcgcaga cgcagagcaa accgtcactg gaatctgtcg
      781 gcgagttcaa cgtgctgtcg aacggcttca gcgcagaata tgccgggatt gccagcgtac
      841 gtgtgagcac aaagcgcggc ggcgcggcgt accacgggtc ggcgttctat aacaacaaga
      901 actcggcact ggcggcatgg agcctggcag acaagaacgc agccgcgtcg tttgcacctt
      961 cgagcttcca gccgcattat ccgaatccat atttcaacat tacggatgtg ggcggatcgg
     1021 ttggcggtcc cgttccccac ttgccgaaaa cgttcttctt tactgcgttt gagcatgact
     1081 ggacggttga gcccacgaca acagggcggg tcaacaacct tccgcatcct gcgctgattg
     1141 ccggcgattt ttcaggagtg aacgactcgg ctaagcctga tgttccggcg ggaaccgttt
     1201 tgacgccgag cgaaattgcg aacgataccg tggatgggct cgggcagaag ttcatcacca
     1261 tcccctcacg cctgctgaac ccattcgtac agaagctctt caacatttac tatccgcacg
     1321 tcggcctgtc ggctccggaa gatcccgcca cgggccgtgt gatcggctac cagacctcga
     1381 ttccaggcca ttccggtgcc aacctggggg atctgcgtat cgatcatgac ttctccgatt
     1441 cgaaccgtct ctatggcgtg tatcacggcg ggtccgagaa cgatgcgctc aacgaagtgg
     1501 cggctcctct tacgggactc ggactgagcc agaccgatcg cctgaataag acactctccc
     1561 tttcgtacac gcacgtcttc ggcccacagc tgatcaatga ggcgcgcgga ggattcaatc
     1621 gccaatacct gttcacccac agcaatacga ctctgcgcgg cttcctgaaa agcatcggat
     1681 tttccgatgc cgatatagcc gcatacggaa gcaccgtggg agcccgtgaa ctggatacgc
     1741 acggtcaggc ggccatcaac atcgccaact tccagggagt aggaaacggc ggccgcaata
     1801 cggagcgtcc gctcagccag aacctcatca cctttggcga cacggtgacg tggaatcccg
     1861 gacgtcacaa tattcgtttg ggcgctgact tcgtgcgcaa ctctgccgag gatggcttca
     1921 cttccgggcg cggccagccg cgcggtctga tcacctacac cggcagcaac acttcggcgc
     1981 ttgcgaatct ccttcttggc aatgctccaa acacggtcag cttcacgccc aagggcaggc
     2041 cggcgatgga cgtatacaac tgggagacgg gcatcttctt ccaggatgat ttccggatga
     2101 acgaccggct cacgttcaat gtcgggctcc gctacgacct gttcacacca ttcaccgaaa
     2161 ccaacgacct gatggtcaat ttcgacccga atctcaccaa tccggtgacg ggcgaacccg
     2221 gcacttacat catgccgtcg agcaagactc tggcgtatgt tgacccaagc gtcattgcat
     2281 tcggatacgt cacggcgcac tcggccggcg ttggacggag ccttgttcac accgactgga
     2341 cagacgttaa cccgcgtctg ggcttcgcct atcgcattgg tggcaaatcg gtatttcgcg
     2401 gtgggtgggg attcttcaca ccgacgtcga ccgcgcacgt gatccgcgat ccactggcga
     2461 ccaatacctt caatcagaca tacaccctgc gcagcacgtc aggcggaccg ccgatcaatc
     2521 catggccgac acagggcgag accacgggaa catcgccgaa taccggcggt acagcgtcgg
     2581 ggtttggaaa cacacctacc gccaacaacg ttccactcga cctgaaaaat ccacggctgc
     2641 aacagtggaa cgccacgttt gaacagcaat tgccgtctgc gtgggtgttc cgcgtttcgt
     2701 acatcggggc ctacatcagc ggcgagatta ccggacgtga cctggacatg attgcgccga
     2761 gcgaccagct gtttggcgtg accacgggtg acggcgtaac catctgcgat cccgccaatg
     2821 aggggaattg cgcttactcc gatgcggaca aagctcgttt gcggtttccg gcgctgggcg
     2881 attacatcac cgccttcgga aacaacggac acgggctgac tgcttccggg caggcccaga
     2941 ttcaacacca gaccaggcac ctgacgctga gtgttgccta cacctacctc aatcagaagt
     3001 cgacgggtgt ggattcggat aacgacagcc tgggcggcga tgcgtataac cagctcgacc
     3061 cgaacagcga ttacgggacg gattcgttcg tatcgcatca ccgagtcgtg gcgtatggag
     3121 tgctcgatct tcctttcggc cgcggccagc gtttcggtga cggcatgcca aaagctctgg
     3181 atgcgatcgt gggtggctgg cagacaacct ttaatatgtt tgccaagacc ggcaccggct
     3241 tcacgccgtt ctactactgc aatgattgcg atcctgtcga gccgggaaac atcgcgtccg
     3301 gcgcgatcga tgcagtgggc gacttcggtg gcggtttccg cgccaagatc atcggcaatc
     3361 caaaccacgg cgcaggcaaa gacctccagt ggaacgccga cgccttcgat cttccggata
     3421 cgggtgccac tcttttcagc aaccctctga atgcgaagcg caattctctt accggccccg
     3481 gtacctacgg cgtgaacctg ggcattcaca agacgttcaa cctcacggat cgcgttggaa
     3541 ttcagatcgg cgcggatgcc gacaatgtct tcaatcaccc cttgctgtcg ccggacagcg
     3601 atagctccga tgacttcgca aatgtcggaa gtttcacgct gcacctcgat ccgaacaccg
     3661 gggcgatcct tcccctggcg acggaatact acccgaatcc tgacttcggc ttgaaatacc
     3721 agagcgtgac gcaggagggt atcgataacc gtcgttccat ccgcctccgc ggacgcatca
     3781 cgttctagga gggcggttcc agaccgaagc ggcaggctaa gaacgacctg ccgctcactt
     3841 ctcctcacga tggcacttca agccagctaa cgtgcagaga agctcgggca gctttcacca
     3901 actctgctct taatggggtt ttctggggta cgcgccgtgt agccgatcgc gcgggattct
     3961 cgttcgctct cgtgcggagt tgcgttgaaa acttcagaag ccggatccga tgtccagaac
     4021 cacgggatcg gtctaccgac gatgacttct tcattggcgg ccgccgagcg aagactatga
     4081 aatcgacagt catccccgac gcaaagtctt atttgcgttt cgagtttgtt ccgatatatt
     4141 acgtatagtt atcttttatc aaagctatga agaataccac catccagcag aacgaagagg
     4201 cgcgacttgg gcccctgtca caactcatgg aactctctca ggtcgagcaa aaggcgagag
     4261 gcctgctttt cactcctcgt gaaattgctc agcagccgga tacctggaag acgacacttg
     4321 cgatcttcag acagcatcag gcggaagtga gcagttttct ggagcacgct ggagtgcttg
     4381 ctccgctgga ggagcggccg gtggtgatgc tggtgggggc cgggacatca gactatattg
     4441 gtcgcgctct cgcgttgctg ctgcgagaga aatggggatg cgaggtttcg gcgtgcgcga
     4501 gcaccgagtt gctgacgaat ttgtcgagct atattgtctc ggggcgtaaa tatttgtgga
     4561 tttcgttttc ccgctcgggg gattcacccg aagcggtggc catcctgaaa caggccatcg
     4621 agcagtaccc tgagatttcc caccttatcc tgacctgcaa cgctcacgca cagatgatcg
     4681 aagtggcgag tgctgctttg cattcgtacg tcgtggtgct ggacgatgcg gtgaacgatc
     4741 gcagcctggc catgacgagt tcatttacaa acatgctgat catgggtcag tgtctcgcac
     4801 atgcgtggtc gatcgaagag tatgcgccga tacttgaaca gttatccgat gcgggccacg
     4861 acttcctccc caaagcatcg cgggaggccg ctgcgacggc tgcgctcctg cttccgcgcg
     4921 cctgttttgt gggttcaggc accctggcag gtgtagccaa ggaatcggct ctcaaggtaa
     4981 cggagatgac cgcaggacaa gttaagtcga tttcagaaac ggtgctcggc ttgcgccacg
     5041 gccccatggc cgctctcgat acagagacat tgtttgtgtg cttcgtttcc agcgatcgaa
     5101 ggaggcaaca gtacgcaagc gatcttttgc aggagattag tcaaaaggga attacagggc
     5161 gaagaattgt agtcggcccc ggttcggcac agcatctgtt c
//
LOCUS       DASZYN010000736         2885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_813829, whole genome shotgun sequence.
ACCESSION   DASZYN010000736 DASZYN010000000
VERSION     DASZYN010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813829"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..1305)
                     /gene="dinB"
                     /locus_tag="VHE33_18975"
     CDS             complement(235..1305)
                     /gene="dinB"
                     /locus_tag="VHE33_18975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HVT99590.1"
                     /translation="MTVRKIVHIDMDAFYASVEQRDDPTLRGKPVIVAWRGNRSVVCA
                     ASYEARRFGVRSAMPALRAERLCPDAVFIPPDFPRYRTVSRQTRDIFRRYTDLIEPLS
                     LDEAYLDVTENKPGLPTATKVASAIRRDIRGELNLTASAGVAPNKFLAKIASDWRKPD
                     GLFVIQPEDIDAFLPPLPVERLPGVGKVTAQHLANLGIKTVGDLRKMELVTIEEHFGR
                     HGRRLHELACGIDENPVVPDRPTKSISAEDTFERDIPLEETADAIRRLAEKVWAASRK
                     ETRIPRTVVLKLKTAEFKILTRSLTPPEPVASCDDLTGIALSLRERVGLAPSQRFRLI
                     GVGLHNFSDPDDNDPAQPILFD"
     gene            1771..>2885
                     /locus_tag="VHE33_18980"
     CDS             1771..>2885
                     /locus_tag="VHE33_18980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99591.1"
                     /translation="MHLLQGYVCKPETTRSNSSSGLTVKTLRWTAGSLAILIASVVLT
                     GCGSIAVNNPNPSQASNGAGSTSGNGTGAGTTGTGSGSGSTGTGTDGSGSTGTGTTGT
                     GTTGTGSAGSGSDGSGSTGSGSTGTGTTGTGSTGSGSGSGASAGALAVVPANALRVDS
                     IQALSNWKADYDSATSGPAGSARGASSLVETPSLSGAARQFTSDYTYYGGERFHASFG
                     NDRTSKHFLYDVWVYLDSSISSVANLEMDMNETMPNGQTVIFGFQCDGWSNTWDYTTN
                     AGTPEVPVDRWLKSDQACNLQDWTLNTWHHVQVEYSRDDVGNVTYQSVWLDDVEQDIN
                     QTTPSAFALGWGSSLITNLQVDGRNATSGTSTMYLDKL"
BASE COUNT          585 a          696 c         1090 g          514 t
ORIGIN      
        1 tccggatcac acgcgggtgg aggtgatgga gttcggagcg aaggcgaagc cttgctgcag
       61 tgagttcacg gcggcgaatc cgacgccgga ggggcagccc tgagaggcag ggaatagcca
      121 gtagccaatc gggagcagga actgcgagaa gaactgcgag cgaactacga gtagaactgc
      181 gagcagaacc acgagcaaac tgcgagtaga actgcgagta gaacggcgcg ttcgttagtc
      241 gaagaggatg ggctgggcgg ggtcgttgtc gtcggggtcg ctgaagttgt ggaggccgac
      301 tccgatgagg cgaaagcgtt gcgatggggc gaggccgacg cgttcgcgca gggagagcgc
      361 gatgccggtg agatcgtcgc aggaagccac gggctcgggc ggcgtgagcg agcgggtgag
      421 aattttgaac tccgcagttt tgagtttgag gacgacggtg cgcgggatgc gggtttcttt
      481 gcgcgaggcg gcccagactt tttcggcgag cctgcggatg gcgtcggcgg tttcttccag
      541 aggaatgtcg cgctcgaagg tgtcttcggc ggagatggat ttggtgggac ggtcgggaac
      601 gacgggattt tcgtcgatgc cgcaggcgag ctcgtgcagg cgccggccgt gacggccaaa
      661 atgctcttcg attgttacta attccatctt gcgcaggtcg ccgacggttt tgatgccgag
      721 attggcgaga tgctgcgccg tcactttgcc gacgcctgga aggcgttcga cgggaagcgg
      781 cgggaggaaa gcgtcgatgt cttcgggctg gatgacgaag aggccgtcgg gcttgcgcca
      841 gtcggaggcg attttggcga ggaacttgtt gggggcgacg ccggcggagg cggtgaggtt
      901 gagttcgccg cggatgtcgc gacgaatggc actggcgact ttggtagcgg tcgggaggcc
      961 gggtttgttt tcggtgacgt cgaggtaagc ctcgtcgagg gagaggggct cgatgagatc
     1021 ggtatagcgg cggaagatat cgcgggtctg gcgggagacg gtgcggtagc gggggaagtc
     1081 gggcgggatg aaaacggcgt cggggcagag gcgttcggca cggagcgcgg gcatagcgga
     1141 acggacaccg aagcggcgag cttcgtagga ggccgcgcag acgacggagc gattgccccg
     1201 ccatgcgacg atgacaggtt tgccgcggag ggttgggtcg tcgcgttgtt cgaccgacgc
     1261 gtagaaggcg tccatgtcga tgtggacgat cttgcggacg gtcatagcga cagggtacag
     1321 ggtgcagggc gcagggcgca gagcacagag cacagggcac agagcacagg gcacagaaca
     1381 cagggcacag agcacagagc acagagcaca gagcacagag cacagagcac agaagtacgc
     1441 gtgttctttg tgaggtggtc gtttgcgggt tgggcgggat gtgcggaggg cgcgaactgc
     1501 ctgatatcgg gatgggaatg accatagccc gaacggggga ggcgaacgcg tgtgcgcgcc
     1561 atgccgggga gtgttacttc cgtggcacgg acgtgttaac tcctctggaa cgaaactact
     1621 aactcaacac attgtggtta ctgcggggtg attcgaccct cgtttgtgtg attggcgata
     1681 caggtacttc cccttatctt cggatcaact accaaacgag aaagcggatc gtggagaacg
     1741 ccaggcatgg aaatgccgcg ggaaagttcc gtgcatttgc tgcaggggta cgtctgcaag
     1801 cccgagacga cgcgctcgaa ttcttcttct gggcttaccg tgaaaactct tcgctggacc
     1861 gctggttctc ttgccattct tatcgcttcc gtcgttctta ccggatgcgg gtcgatcgcc
     1921 gtcaacaatc caaacccctc ccaggcgagc aatggggcgg gaagcacttc cggaaatgga
     1981 acgggtgcag gtacgacagg cacaggttcg gggtcaggca gcacaggcac gggaacggat
     2041 ggatcggggt cgaccggcac tggaacgacg ggcactggaa cgaccggaac gggcagcgcg
     2101 ggttcgggca gcgacggatc ggggtcgacc ggcagcggat cgacgggcac tggaacaact
     2161 ggaacgggca gcacgggttc gggttccgga tcgggagcct cggccggcgc attggcggtg
     2221 gtgccggcga atgcgctgcg ggtggattcg attcaggcgt tgagcaactg gaaggcggac
     2281 tacgactcgg ccacgagcgg gcctgccggt agtgcgcgcg gagcgagcag cctggtggag
     2341 acgccgtcgc tgagcggtgc ggcgcgacag ttcacttccg actacacgta ttacggcggg
     2401 gagagatttc acgcttcgtt cggaaatgac aggacgtcga agcatttcct gtacgacgtg
     2461 tgggtgtatc tggacagctc aatcagcagc gtggcgaacc tcgagatgga catgaacgag
     2521 acgatgccga acggacagac ggtgattttt ggatttcagt gcgacgggtg gtcgaacacg
     2581 tgggactaca cgacgaatgc cgggacaccg gaggttccgg tggatcggtg gctgaagtcg
     2641 gatcaggcgt gcaatctgca ggactggacg ctgaatacgt ggcatcacgt gcaggttgag
     2701 tactcgcggg acgatgtggg gaacgtgacc taccagtcgg tgtggctgga tgacgtggag
     2761 caggacatca accagacgac accgtcggcc tttgcgctgg gatggggatc gtcgctgatc
     2821 acgaacctgc aggtggatgg tcgcaacgcc acgagtggaa cgtcgacgat gtatctggac
     2881 aagct
//
LOCUS       DASZYN010000737         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1262962, whole genome shotgun sequence.
ACCESSION   DASZYN010000737 DASZYN010000000
VERSION     DASZYN010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262962"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="VHE33_18985"
     CDS             <1..1545
                     /locus_tag="VHE33_18985"
                     /inference="COORDINATES: protein motif:HMM:NF027309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HVT99592.1"
                     /translation="YETILRLKGNMVVPGTWIFPDDPQIKAAAERGLIVNQHHAIPLG
                     LNVARWPKDVPYNFSTHPEVLERAWKDAVAEYPPNIELLWDVGLRGLSDTSYASMDPS
                     VQGNDELLGKRISDAIEEQMKIVRAVHPDAKFVTDLWQEGARLVQEGYLKIPPEVTTV
                     WADDGYGYIQDKGQVKAGEGTYYHVAMMNNRTNQLTEMVPIERISEELGRYVKAGATQ
                     YFLVNTSDIRPYALTIRAVMDYVWLGKLPGVEKEPSEFYRHYAAEEFGEKAAGAIADM
                     YKDYFAAPALLERRNQDDPPREYGDQLYHTEGRQMLQTWMIDAPLYAIGGQSPKWTIP
                     RLVGEGYGRPGFGGKSWLKTAVPREIEQCGDAQPRWDAVWKKAVAAEALVPASRREFY
                     QASVLTMVTIQRESNRMLADIAKSLQDAQKGDAAGAQREAADALVAVGEVCKAQAAAE
                     YGKWKNWYRGDWLTGVYRTQQVLEDYAGFLKDPESHLPPPILWSGWEAYYHIMQYEGD
                     RSADVR"
     gene            complement(1643..>2345)
                     /locus_tag="VHE33_18990"
     CDS             complement(1643..>2345)
                     /locus_tag="VHE33_18990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99593.1"
                     /translation="ADRWFPAPITLATTPGEHRFIWDLASGAASASPDDDDEDAAPGG
                     PKVAPGNYTLRLNVDGSSLDRPLRIVMDPRATATAAVLTQQYALAESIYAQVLESRKA
                     MAELESVDSQLKKLAASSNTSEDVRSAVRAAETKLAAIRTGGHTETAPGLAEAASGLG
                     TVLRIVESGDREAPASALAIFAQMKKASADDIAAWHRFKTADLTTLNNALTAAHREPI
                     HIAAIEEEVHYAMTR"
BASE COUNT          455 a          561 c          887 g          442 t
ORIGIN      
        1 tatgagacga tcctgcggct gaagggaaac atggtggtgc cggggacatg gattttcccg
       61 gacgatccgc agatcaaggc ggcggcggag cggggactga tcgtgaatca gcatcatgcg
      121 atcccgctgg gactgaatgt ggcgcgatgg ccgaaggatg tgccgtacaa cttctcgacg
      181 cacccggaag ttctggaacg ggcgtggaag gatgcggtgg cggagtatcc gccgaatatc
      241 gagttgctgt gggatgtagg tttgcgcgga ctttcggata cgagctacgc gtcgatggat
      301 ccgagcgtgc agggcaatga cgaactgctg ggaaaacgga tcagcgacgc gatcgaagag
      361 cagatgaaga ttgtgcgcgc ggtgcatccg gacgcgaagt ttgtgacgga cttatggcag
      421 gagggtgcgc ggctggtgca ggagggctac ctgaagattc cgccggaggt gacgaccgta
      481 tgggcggacg atgggtatgg gtatatccag gacaaaggcc aggtgaaggc cggggagggc
      541 acctactatc acgtagcgat gatgaacaat cgcacgaacc aactgacgga gatggtgccg
      601 atcgaacgaa tctcagaaga gctggggcgc tatgtgaagg cgggggcgac gcagtatttt
      661 ctggtgaaca cgagcgacat tcgcccctat gcgctgacga ttcgggcggt gatggactac
      721 gtgtggctgg ggaaattgcc gggcgtggag aaggagcctt cggagtttta tcggcactat
      781 gctgcggagg agtttgggga gaaagcggcg ggcgcgattg cggatatgta taaggactat
      841 ttcgccgcgc cggcattgct ggagaggaga aaccaggatg atccgccgcg ggagtacggg
      901 gaccagctgt accacaccga aggccggcag atgctgcaga catggatgat cgatgcgccg
      961 ctgtatgcga ttggagggca atcgccaaag tggacgattc cgcggctggt tggtgaggga
     1021 tacggacggc cgggatttgg cggaaagtcg tggctgaaga cggcggtacc gcgagagatt
     1081 gagcagtgtg gcgatgcaca gccacggtgg gatgcagtgt ggaagaaggc cgtggcagcc
     1141 gaggcgctgg ttccggcgtc gcggcgggag ttctatcagg catctgtgct gacgatggtt
     1201 acaattcagc gcgagagcaa tcggatgctg gcggacattg cgaagtctct gcaggatgcg
     1261 cagaagggcg acgcggccgg ggcgcagcga gaggcggcgg atgcgcttgt tgctgtgggc
     1321 gaagtgtgca aagctcaggc ggccgctgag tacggcaagt ggaagaactg gtatcggggc
     1381 gactggctca cgggtgtgta tcggacgcag caggtgcttg aagattatgc gggattttta
     1441 aaagatcctg agtcgcacct gccgccgccg atcttgtgga gcggatggga ggcttactac
     1501 cacatcatgc agtacgaggg ggaccggtcg gcggatgtgc ggtgaagaca gcgttcggcg
     1561 ttcagcgctc agcgttcagc aaaagcgcgc gctgacgcgc gattcccatg tactaacacc
     1621 agcgacatgg ggcaccccga cctcatcgcg tcatcgcgta atgaacctct tcctcgatag
     1681 cggcgatgtg gatgggctcg cgatgcgcgg ccgtcagtgc attgttgagc gtggtcaggt
     1741 ccgctgtctt gaagcgatgc caggcggcga tatcgtccgc tgaagctttt ttcatttgcg
     1801 cgaagattgc gagagctgat gctggagctt cgcgatcgcc gctttcgaca atgcggagga
     1861 cggtgccgag gccggaggcg gcttccgcga ggccgggcgc ggtttcggtg tggccgccgg
     1921 tgcggattgc ggcgagcttg gtttcggcgg ctcggacggc gctcctgacg tcctccgaag
     1981 tgttggagga ggcggcgagt tttttcagct ggctgtcaac gctttccagt tctgccatgg
     2041 ctttgcggct ttcaagaacc tgcgcgtaga tggattcggc cagggcgtat tgctgcgtga
     2101 gcacagcggc ggtagccgtg gcgcgtgggt ccatgacgat gcgaaggggc cggtcgaggc
     2161 tcgagccatc gacgttgagg cgcagcgtgt agttgccggg ggcaactttg ggcccgccgg
     2221 gggcggcatc ctcgtcgtca tcgtcgggtg aggcgctcgc ggcacccgag gcgagatccc
     2281 agatgaagcg atgctcgccg ggagtggtgg cgagggtgat cggagcgggg aaccagcgat
     2341 cggcg
//
LOCUS       DASZYN010000738         1994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_742936, whole genome shotgun sequence.
ACCESSION   DASZYN010000738 DASZYN010000000
VERSION     DASZYN010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742936"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..>1994)
                     /locus_tag="VHE33_18995"
     CDS             complement(176..>1994)
                     /locus_tag="VHE33_18995"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HVT99594.1"
                     /translation="HYTPDMPHAAAAAVKAGTDLECGYGQGQAFPSLVDAVHQGLITE
                     AEIDTALHRLYRARFELGMFDPLESYAYGKIPFSEVNSPQHRQLSLQAARESMVLLKN
                     EDHTLPLQSSIANIAVIGPTAEYVQSLQGNYNGPPPNPVYPLNGIEKRFSSAHVTYAQ
                     GSTLVDGYAMPIEHTALHTSDGRGDGLTGQYFASPDFAGKPVLTRTDRNINFNWDKVV
                     PVPGLARNNYSVRWTGTLTPPAPGDYKLGVHINYCYACENAEGFQLYLDGKQIVASSS
                     HMSGERGAVYEAAVHFDNTQPHKIRLDYLHGTGTAGIDLTWQAPGDVLRNQAVEAAKN
                     ADVIIACVGLSPNLEGEEMPVQLEGFKGGDRTSINLPAAQEDLLKALAGTGKPLVVVL
                     QNGSALAVDWAQQHANAILEAWYPGEEGGTAIAETLAGDNNPAGRLPLTFYASDSQLP
                     PFHEYSMQNRTYRYFDGKPLYPFGYGLSYTSFAYSNVKTPHEVEAGQSIQLEADVKNT
                     GSTAGDEVVEVYLKQPSGYQTPIHELAGFTRVHLDAGATTHVGLTLDPRAIAQVDEKG
                     NRVILPGEYTVSVGGGQPGTSAANQTATFTITGRREFPK"
BASE COUNT          334 a          530 c          722 g          408 t
ORIGIN      
        1 gcaagagcag cagcagactt caggaacgtg cgacgtttca tggatagcct cgggaggacg
       61 ccaacaaggg tatcaagtga aacgtttaag tgggaaggaa ttttgacgaa cgtcctcacc
      121 tgcggtactc acgggtggcg aaccgccggg atgggaatcc gctgtcagtt ttcgcctatt
      181 tcgggaactc tctcctgccc gtaatggtaa aagtggcggt ttggttcgca gccgacgtgc
      241 ccggctggcc gccgccgacc gacacggtgt attcgccggg gaggataacc cggttaccct
      301 tctcatcaac ctgggcgatg gcccgcggat cgagggtcag acccacgtgg gtggtcgcgc
      361 ccgcatcgag atggacgcgt gtgaagccgg ccagttcgtg gatcggcgtc tggtagccgc
      421 tgggctgctt gaggtagacc tccacgactt catcgcctgc ggttgagccg gtattcttca
      481 cgtcggcttc gagctggata gactgcccgg cctcgacctc atgcggcgtt ttcacattgc
      541 tgtaggcaaa gctggtgtag ctgagcccgt aaccgaaggg atagagcggc ttgccgtcga
      601 agtagcggta ggtgcggttc tgcatggagt attcgtggaa gggcgggagc tgcgagtccg
      661 aagcgtagaa cgtgagcggc agccggccgg caggattgtt gtcgcccgcg agggtttcgg
      721 cgatggcggt gccgccttct tcgccggggt accaggcttc gaggatggcg ttcgcgtgtt
      781 gctgtgccca gtcgaccgcg agggccgaac cgttttgaag cacaacgacg aggggtttgc
      841 cggtccccgc cagggctttc agcagatcct cctgggcggc gggcaggttg atcgaagtgc
      901 gatcgccgcc tttgaatcct tccagctgaa cgggcatctc ttcgccttcg agattgggtg
      961 ataggccaac gcaggcgatg atgacgtcgg catttttggc tgcttcgaca gcctgattgc
     1021 gcagaacgtc tccgggagcc tgccaggtga ggtcgatgcc ggcggtgcct gtgccgtgca
     1081 ggtagtcgag ccggatctta tggggctggg tgttgtcgaa gtgcacggcg gcttcgtaaa
     1141 cggctccccg ctccccggac atgtggctgc tgctggcgac gatctgcttg ccgtcgaggt
     1201 agagctggaa tccctcggcg ttttcgcagg cgtagcagta gttgatgtgg acgccgagtt
     1261 tatagtcccc gggagcgggc ggggtcaggg tcccggtcca gcgcacggag tagttgttgc
     1321 gcgcgaggcc ggggacggga acgactttgt cccagttgaa attgatgttg cggtcggtgc
     1381 gggtgagcac gggcttgccg gcgaagtccg gtgaggcgaa gtactggccg gtgaggccgt
     1441 ctccacggcc atcggaggtg tggagcgcgg tgtgctcgat cggcatagcg taaccatcca
     1501 ccagcgtgga tccctgggcg taggtgacgt gtgcggagga gaagcgcttc tcgatgccgt
     1561 tgagcgggta gaccgggttc ggcggcggac cgttgtagtt gccctgaagg gactggacgt
     1621 attcggcggt ggggccgatg acagcgatgt tcgcgatgga agactgcagc ggcagggtgt
     1681 ggtcttcgtt cttgagcagg accatcgatt cgcgcgcagc ctgcagcgag agctgacggt
     1741 gctggggcga attgacctca ctgaacggaa tcttgccgta agcgtagctc tcgaggggat
     1801 cgaacatgcc gagctcgaac cgggcacggt acaggcggtg gagcgcggtg tcaatttcgg
     1861 cttcggtgat caggccctgg tgcacggcgt ccaccagcga cgggaaggcc tgaccctgtc
     1921 catacccgca ttcgagatcg gtgccggctt tgacggcggc tgcggcggca tgaggcatgt
     1981 ccggcgtgta gtgg
//
LOCUS       DASZYN010000739         3527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1072919, whole genome shotgun sequence.
ACCESSION   DASZYN010000739 DASZYN010000000
VERSION     DASZYN010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072919"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1382)
                     /locus_tag="VHE33_19000"
     CDS             complement(66..1382)
                     /locus_tag="VHE33_19000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99595.1"
                     /translation="MSGYRRPAGFSLLAAFCALPLLAALPLHAQVFTVEAEHVEKHYT
                     EFAPTHVRYPEQPLNQLGRELLIRFMQSEQGFAMRPLPIANLDLHANGHMDPTGEGYV
                     DLLHKKGTSAKPGDRVVVTDIKINDKSIQLDLNGGFQHKHRILRHISIGMDPVNTTPL
                     AHDDGQQPTGSRITLVFEERVPNLNGEQLQALLRPMIDFGVKSPAEAFAETLPDFLRA
                     AIEQHRVLVGMNRDMVVYAKGQPIRKERETGEDGKQFEIWIYGESPQPVEFVRFNGTY
                     VAEVKLAKVGEPMIVQNQNEMGDYWGNQPVVATNQREIRLGDQSDTDRSTEGAPRHAP
                     TLRKPGEELPQDSGKNPVPTMRPVNMPPDLQRPGDPGYHPTVSAPPEQGSAAPQPQTE
                     SPASTTQTQSTSNGTKDTSKPSQTKPSQPQPNNSTDNGPNPLDNPH"
     gene            complement(1568..1834)
                     /locus_tag="VHE33_19005"
     CDS             complement(1568..1834)
                     /locus_tag="VHE33_19005"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HVT99596.1"
                     /translation="MQTPDRPDDMRELIAQIARALVDIPGEVQVEEQLHGDATNLALR
                     VARSDVGKVIGKQGRTARSMRTILAAVSMKHRHRYTLDILEEEP"
     gene            complement(2136..>3527)
                     /locus_tag="VHE33_19010"
     CDS             complement(2136..>3527)
                     /locus_tag="VHE33_19010"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HVT99597.1"
                     /translation="ILDMRLDERTALLAAGLADAKEELKFLALHDSLTKLPNRTLLAD
                     RLEQEIQNAQREQSRFTVLSMDIDGFRQINDAWGMATGDQLLIEIAARLRESLRARDT
                     IARLGGDEFVVLADTQQPGDAAQLAEKLLDIVRQPIVIDGHELRVSMSIGIAIYDGRE
                     PLPGDALRNADAAMNHAMALGHNCYVFFESSMSDDAQNQLQMVQDLRRAEERGELVLH
                     YQPKYAAEDGRLIGAEALLRWRHPLRGLVPPGDFIPLAEKTGLIFQIGRWVLDEACRQ
                     MRAWRDAGHQGWTLSVNLSALQFNHPDLIDLVRISLERYRLEPHYLTLEITESTAMHD
                     ANNSLAILQQLDQMGVRISIDDFGTGYSSLLYLKRLPASELKIDRGFVRDLMHDTEDA
                     AIITAIVALGRALGLKIVAEGVETREQQEFLKRLGCTSLQGFLLGRPMPADEFEKAVV
                     WRESSWRAEQLLA"
BASE COUNT          614 a          910 c         1287 g          716 t
ORIGIN      
        1 actgaagtca tgccctgtta caaaagcgcc cggccaattc ttcaacgaac ttgagggacg
       61 ccacactagt gcgggttgtc gagggggtta gggccgttat ctgtcgagtt atttggctgt
      121 ggctgggaag gcttcgtttg cgatggtttt gaggtgtctt tggttccgtt gctggtgctc
      181 tgcgtctgtg ttgttgaggc tggagactcc gtctgcggct ggggagccgc acttccctgc
      241 tccggcgggg cggagaccgt ggggtgatag ccgggatcgc cgggcctctg gagatcggga
      301 ggcatgttca cggggcgcat ggtgggcacc gggttcttgc cggaatcctg gggcaattct
      361 tcgccgggtt tgcggagcgt gggtgcgtgg cggggcgcgc cttctgtgga gcggtcggta
      421 tcgctctgat cgccgagcct gatctcgcgt tggttggtgg cgacgacggg ctgattgccc
      481 cagtagtcgc ccatctcatt ctgattctgg acgatcatgg gctcgccgac tttggccagc
      541 ttcacttctg ccacataggt gccgttgaag cggacgaatt cgacgggctg cggcgattcg
      601 ccgtagatcc agatctcaaa ttgcttgccg tcctcgccgg tctcgcgctc tttgcggatg
      661 ggctggcctt tggcgtagac gaccatgtcg cgattcatgc caacgagaac gcgatgctgc
      721 tcgatggcgg cacggagaaa atcggggagc gtttcggcga aggcctcggc gggcgatttc
      781 acgccgaagt cgatcatggg cctgagcagt gcctggagct gctcgccatt caggttgggg
      841 actcgctctt cgaagacgag ggtgatgcgg gatccagtgg gttgctggcc gtcgtcgtgg
      901 gccagcgggg tagtattcac gggatccatg ccgatggaga tatgacgaag gatcctgtgc
      961 ttgtgctgga agccgccgtt cagatccagc tggatgctct tgtcgttgat cttgatatcg
     1021 gtgaccacca cgcggtcgcc gggcttagcc gaggtgcctt ttttgtggag gaggtcgaca
     1081 tagccctcgc cggtgggatc catgtgcccg ttggcatgaa ggtcgagatt ggcgatgggc
     1141 agggggcgca tggcgaagcc ctgctcggac tgcatgaaac ggatgagcaa ttcgcggccg
     1201 agctggttga ggggctgctc gggatagcgg acgtgggtgg gggcaaattc ggtgtagtgc
     1261 ttctcgacgt gctcggcttc aaccgtgaag acctgagcat gcagggggag cgccgcaagg
     1321 agggggagcg cgcagaacgc cgcgaggagg gagaagcctg cgggacgtcg atagccggac
     1381 atagagcgcc ttctgtgcgt gggagccaga caccaccagc atggccccgt tttgaggaaa
     1441 attcaagcgg gacagcgggt ccatatccac ttcaatgagg gtcggctggc cacatcccct
     1501 gttttggagc cggaagcttg ccaggccgtt gccaggcgtg aaggggtggc gcggattgtc
     1561 gcgcggttca gggctcttct tcgaggatgt ccagggtgta acggtgacga tgcttcatgc
     1621 tcacggccgc caggatggtg cgcatagagc gggcggttct gccctgcttg ccgatcacct
     1681 taccaacatc ggagcgggcc acgcggaggg cgaggttggt ggcatcgccg tgaagctgtt
     1741 cctcaacctg gacttcgccg ggaatatcca ccagggcgcg cgcgatctga gcgattaact
     1801 cgcgcatgtc atcggggcga tcgggagttt gcatcgttct tttgggtgga acgcgaaggc
     1861 agcattgaag tcggctgaac ggccggtacg gttagggaaa acctaaccgg catcgtcgag
     1921 gaaggatgtg accgccatca caatcgaagg agtgctatgg atttgaagac cagccggggc
     1981 gccaggccag tgtggtttcc cggaaggaat ttcaactatg gaacgggccg aaaagcaggt
     2041 tcctcggctc accaccccca aactgaaata cgtttggggc ccctttcgct cggaatgaca
     2101 agcctctttt gtaaacaaac ctttgggacg ccgcactagg ccaggagttg ttcggcgcgc
     2161 caggaggatt cgcgccacac gacggctttt tcaaactcgt cggcgggcat gggacggccg
     2221 aggaggaagc cctggagaga ggtgcaaccg aggcgcttga ggaattcctg ctgctcccgg
     2281 gtctcgacac cttcggccac gattttgagg cccagagcgc gaccgagcgc cacgatggcg
     2341 gtgatgatgg cggcgtcttc ggtgtcgtgc atgagatcgc ggacgaagcc gcgatcgatc
     2401 ttgagctcgc ttgcggggag gcgcttgagg tagaggaggc tggagtagcc ggtgccgaaa
     2461 tcgtcgatgg agatgcggac gcccatctgg tcgagttgct ggagaatcgc caggctgttg
     2521 ttggcgtcgt gcatggcggt cgattcggtg atctcgaggg tgaggtagtg gggctcgagg
     2581 cggtaacgct cgagcgagat gcgcacgagg tcgatgaggt cgggatggtt gaactgcaag
     2641 gcggagagat tgaccgaaag ggtccagccc tgatggccag cgtcgcgcca ggcgcgcatc
     2701 tggcggcagg cttcgtcgag gacccagcgg ccgatctgga agatgaggcc ggttttttcg
     2761 gcgaggggga tgaaatcacc gggaggaacc agtccgcgga ggggatggcg ccagcggagg
     2821 agggcttcgg ctccgatgag gcggccgtct tcggcggcgt atttgggctg atagtgaagc
     2881 acgagctcgc cgcgctcctc ggcacggcgc agatcctgga ccatctgcag ctggttctgg
     2941 gcgtcgtcgc tcatggacga ctcgaagaag acatagcagt tgtgtcccag ggccatggca
     3001 tggttcatgg cggcgtcggc attgcgcagc gcgtcgccgg gaaggggttc acggccgtcg
     3061 tagatggcga tgccgatgct catggagacg cggagttcgt ggccatcgat cacgatgggc
     3121 tggcgaacga tatcgagcaa tttttcggcg agctgagcgg catcgccggg ctgctgggtg
     3181 tcggccagga cgacgaactc atcgccgccg aggcgggcga tggtatcgcg ggcgcgaaga
     3241 gattcacgca ggcgagcggc gatctcgatg agcagctggt cgccggtggc cataccccag
     3301 gcatcgttga tctggcggaa gccgtcgatg tccatggaga gcacggtgaa gcggctctgc
     3361 tcgcgttgcg cattctggat ttcctgctcg agacgatcgg ccagcagggt gcgattgggg
     3421 agcttggtaa ggctgtcgtg gagggcgagg aacttcagtt cctctttggc gtcggcgagg
     3481 ccggctgcca gcagggcggt gcgctcgtcg aggcgcatgt cgaggat
//
LOCUS       DASZYN010000740         1595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_421886, whole genome shotgun sequence.
ACCESSION   DASZYN010000740 DASZYN010000000
VERSION     DASZYN010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421886"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          332 a          426 c          519 g          318 t
ORIGIN      
        1 ggcctgcccg gcggctccat gcggcgcgag gacggcagcg ctgccgtttc cggcgtgggg
       61 ccgaacccgg accgggacga gcaggaagtg gcggccgagc gcaaggaccg cgaagctgag
      121 aggcatgact cccagaagaa gtaactgttg taacatgtga cttgtgcctc ccaagaagcc
      181 taagtgcctg catccaactc ggaatggtcc ttgcggtctt cttgaggggc acaagtctgc
      241 tcaccgcagt gagacgtcgc ttgcccggat ctacgctgct cagtccgcta aggaccgggc
      301 ttggcgggcg gctaacccgg aacttgcccg ggttaaggct cgtgaaagcg cccgagcgtg
      361 gcgatcggct aatccaggat acgacgtaga gcgccgcgac agatattacg accgtgagcg
      421 tcatgcaaag cgtgagtaca gtaggcggta caagaagcgt gttgcgctgc tccctgggtt
      481 ctgctgggta gaggcgcctc gcctcaggaa gcatgcgaat aaatacgcgg acccggagaa
      541 gcttcgtcaa gcactgctgt ccgggtcgca gagatcggct gcggctaagg ggcgagcggc
      601 cgaatcctcc tttagggact ggtgtgctgc tcacggctac cggctagtgc cttatgcagc
      661 ctggaagggc aataaggctc ggtatgcagc tgtgtgccct aacggccacg agtgcaactt
      721 gaagcctaac catgtgctca gcggggttgc agcttgccgc ctgtgtaggg ctaagaacga
      781 ctgggattcg gtttatattc ttatcaaccg atttgccggc gagttgaagt ttggaatatc
      841 gggaagcatt cgcccgcgac tagcctcaca ccgaaaagac ggatttacag aggtctactt
      901 ggtctgcggt tttcctgatc cgcttaccgt agagaacgcc gtcaagcaga cgctgaagct
      961 agcaggggag acgcccgtgc gagggtatga gtatttccct atatctacac ttgcgctagt
     1021 ggaagatgtt atctggcagt acgaggagga agctagtgcc tgcacggagg aggcgccccc
     1081 cgcaatctaa agcggctgat cgtaaccgta acggagctga gggttccatt tggcggcgga
     1141 tgacagaata tgttgtggac agagacgctg gtatttgcag aatttgtttg cacccgggag
     1201 ccaaatcggc tgatcataag atccccgtta ctgagcgtcc ggatcttgca atggcggcct
     1261 ctaatttgca ggccgtccat ggctggccta acttctgccc ggtctgcagc gctgcgtcgc
     1321 aggccagggg tggaaagcga atctactgca acgaactgaa gagcaacggg actacggagc
     1381 gtgcccgccg gatcatcagc gaacgcactg gcctggtcct tgattcgaaa actgtggacc
     1441 aggccagtgg cgaacgcgag tggtgatcag ccgggcgtgc agtcagtggg tggcgtgatt
     1501 ggcatggccc ggttaatgcc agctggcagt aaggggtcag tcctcgtcgg ccgagaacgt
     1561 gatgtccccg atccggtcgt tccggccctt gacgc
//
LOCUS       DASZYN010000741         5411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_176278, whole genome shotgun sequence.
ACCESSION   DASZYN010000741 DASZYN010000000
VERSION     DASZYN010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176278"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(347..2779)
                     /locus_tag="VHE33_19015"
     CDS             complement(347..2779)
                     /locus_tag="VHE33_19015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT99598.1"
                     /translation="MSDIWGDLRVAVRRLRHAPGFVLVAVLSLTMGIVANLVVFGVLN
                     AAILRPLTGSGAGRVWTIEHKEHGYISHSYPDFQDLRAGNSTFSDMAAFRIGEAALSV
                     GGLTKKSWMYQVSGSYFDMLGVEPEAGRLFHASDEHGPNSAPFVVLSDGYWRARFAAD
                     PRIIGTTVDLNKHPFTVIGVAPPEFHGTELFLWPDFWVPMVNQEQLDGYDNLHKRYNH
                     SIFVVGELKPGVTMEQASNDLNTVAARMVKQNPVDDDGLGFRLVKAGLFGDQVGEAAR
                     SFLAALLLLALLVLAAACVNLASIFAARAADRGRELAIRVAIGSSRWRVLRQVLAEAA
                     LLAIGGGAIGTMASVALMKVMSQWHPVPALPIHVTVPADARVYLIAVLLAAASCVLPA
                     VLTARQIWQIDAMQVMKGAAQPIFRRMTMRDALLGLQVALCALLVTCALVGLRGMSRQ
                     LHAPIGIQPEGVVLAHTEMKMAGYSNESALPVEKRMLEEAAQIPGVTAVGTIDEPQLN
                     AGGSSTPVYREGTQDFRGSNSVFTSKYFTISPGYLEAAGTRLVAGRDFTWHDDKQAPK
                     VSLVNETFARTLFGSAQAAIGRHYAEPGAHNATLYEIVGVVEDGKYDALTEAQQTAMY
                     WPLAQNNENDVALVVRSKRTPAEIVAALDTMMTKIDPSLPVTIQSWPDALALVLLPSR
                     VATVALGILGLLAGILAATGIFGMASYAVARRLREMGIRVALGAQRMQVLRAALGRTL
                     LLLGIGSVAGLALGALGTRVLASIVYHATVYDPLVVIGAIGAMVLIGLMAAAVPARRA
                     VSVEPAALLREE"
     gene            3032..4141
                     /locus_tag="VHE33_19020"
     CDS             3032..4141
                     /locus_tag="VHE33_19020"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HVT99599.1"
                     /translation="MLRRLVLAFSFLLAACLPAQAAPHRQDTGFLNRSLVVNGTLRRY
                     VVYLPDTWNKQQAWPVILFLHGSGERGSEGMDETQIGLPQSIRLHPERWPFVVVMPQV
                     PFSHHHWTDPDMMTMAIAALDAEVKEFHGDRDRVYLTGLSLGGYGVWEIAKAYPHRFA
                     ALVPVCGGVFWSYQPDRWREVNTLPAEYARFVGRTPTWIFHGADDPVVNPRQSRLMFA
                     ALKASGGDVRLWEYAGIKHNVWDRAYGEPQLPHWLLAQRLSAIPKLSPLAEEILVPVH
                     PVPAKVDPNLYNTYTGEYEDQNEVVATVFRQGDRLFSRNRIGDVNELLPESPTTFFYP
                     TGGTRRLTFERDAEGRITGIRFRDDRHEEFWERKR"
     gene            4250..4711
                     /locus_tag="VHE33_19025"
     CDS             4250..4711
                     /locus_tag="VHE33_19025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99600.1"
                     /translation="MAAGVRLLLPMKPCFPAEESVQTNEEKIMKGLLYAAAILALLSV
                     PAFAGAKTQRQTITFSNPVTVGTANFVPGDCKVSWDDTGDTVQVTLTQKANDASVTVP
                     AKVVKGDFPHRSLVLTTGDGGQVLTAIQFNHYSLVLNGTEMASESAPKTDQ"
     gene            5168..>5411
                     /locus_tag="VHE33_19030"
     CDS             5168..>5411
                     /locus_tag="VHE33_19030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99601.1"
                     /translation="MKGFFKYSLFALVFTLLVTPLFAKTHTVRIQQAVTVGSTQIPAG
                     DYKLSWEGTGPMVKVTLAQRGATPVVLDAKLVTGNKN"
BASE COUNT         1045 a         1858 c         1525 g          983 t
ORIGIN      
        1 ggcctccgaa caaatccccc ctgatggcat ctgaggtcga atccacattt tggatagctg
       61 actagcgcag gattcgtgta cacctggttt cgtgaagaac attaccctcc gagccgacga
      121 aaacctcatc aaagacgcct acgcaatggc ccgttccgag ggcaaaagtt tgaatgcggc
      181 gtttcgcgaa tggctgacgc agtatgcggg aagacagatg cagagcaggg aaactgaggc
      241 gcttttcgaa caccttcgag ccgggaactc aggcggccgc cgctttacgc gagacgagat
      301 gaatgagcga cggtaggcct tttctggata gaggtaggaa ccgccctcac tcctcccgca
      361 gcagcgccgc cggctccacg ctcaccgccc gccgcgccgg caccgcagcc gccatcagcc
      421 caatcagcac catcgcgcca atggctccaa tgaccaccag cggatcgtag acggttgcgt
      481 gatacacgat gctcgccagc acgcgtgtgc ccagcgcgcc cagcgccaga cccgccaccg
      541 atccaatgcc cagcagcagc agggttctgc ccagcgcagc ccgtagcacc tgcatgcgct
      601 gggcaccgag cgcgacgcga atccccatct cccgcagacg ccgcgccacc gcgtagctgg
      661 ccatgccaaa gatccccgtc gccgccaaaa tgccggcgag tagtccgaga atccccagcg
      721 ccaccgtggc gacacgcgac gggaggagca ccagcgccag cgcatccggc cagctttgga
      781 tcgtcaccgg caggctcgga tcgatcttcg tcatcatcgt gtcgagagcg gccacaattt
      841 ctgcgggcgt gcgtttcgag cgcaccacca gcgccacatc gttctcgttg ttctgcgcca
      901 ggggccagta catcgccgtc tgttgcgcct cggtgagggc atcgtatttt ccgtcttcaa
      961 cgacgccgac aatttcgtac agggttgcgt tgtgtgcgcc gggttcggcg tagtggcgtc
     1021 cgatcgccgc ctgggcgctc ccaaataatg tgcgtgcaaa ggtttcgttc accagcgaga
     1081 cctttggggc ctgcttgtcg tcatgccagg tgaaatcgcg accggcaacc agccgcgtgc
     1141 ccgcagcctc cagatacccc ggcgagatgg tgaagtactt cgacgtaaaa acgctgttcg
     1201 aaccgcgaaa gtcctgggtg ccctcgcgat acaccggtgt gctgctgcct ccggcgttca
     1261 gttgcggctc gtcgatggtg cccaccgccg tcacgcccgg gatctgcgca gcctcctcga
     1321 gcatgcgctt ctcgaccggc agcgccgact cgttcgagta ccccgccatc ttcatctccg
     1381 tatgcgccag cacaacgcct tcaggctgga tgccgatggg cgcgtgcagc tggcggctca
     1441 tgccgcgcag ccctaccagc gcgcaggtga ccagcagcgc acacaaagcc acctgcagtc
     1501 ccagcagcgc gtcccgcatc gtcatccgcc ggaagatcgg ctgcgcagcg cccttcatca
     1561 cctgcatcgc gtcaatctgc cagatctggc gagccgtcag caccgccggc aacacgcaac
     1621 tcgccgcggc cagcagcacc gcgatgaggt acacgcgcgc atcggccgga accgtcacat
     1681 gaatcggcaa agccggaacg ggatgccact ggctcatcac tttcatcagc gccaccgacg
     1741 ccatggttcc gatcgctcca ccgccaatgg ccaacagcgc tgcctccgcc agcacctggc
     1801 gcagcacccg ccaccggctc gagccgatcg ccacacggat cgccagctca cggccgcgat
     1861 ccgctgcacg cgccgcgaag atgctcgcca gattcacgca cgccgccgcc agcaccagca
     1921 gagccaggag taagagtgcc gcaagaaaac ttcgcgccgc ctctccgacc tgatcgccga
     1981 acagtcccgc cttcaccagc cggaacccga gcccatcgtc atcgaccgga ttctgcttca
     2041 ccatccgcgc cgcgaccgtg ttcaggtcgt tcgacgcctg ctccatcgtt acgcccggct
     2101 tcaattcccc aacgacgaag atgctgtggt tgtaccgctt gtgcaggttg tcgtaaccgt
     2161 ccagctgttc ctggttcacc attggcaccc agaaatccgg ccacaggaac agttccgttc
     2221 cgtgaaactc tggcggagcg acgccgatca cggtgaaggg atgcttgttg aggtcgaccg
     2281 tggtgccgat gatccgcgga tcggccgcga aacgcgcccg ccagtacccg tcgctcagca
     2341 cgacgaacgg cgcggagttc ggcccgtgtt cgtcgctggc gtgaaacagc cgtcccgcct
     2401 ccggctcgac gcccagcatg tcgaagtagc tgcccgacac ctggtacatc cagctctttt
     2461 tcgtcaggcc gccgacactc agcgccgctt caccgatgcg aaacgccgcc atgtcgctga
     2521 aggtcgaatt gcccgcgcgc aaatcctgga agtcggggta cgagtggctg atatagccgt
     2581 gctccttgtg ctcgatcgtc catacccggc ctgctccgga gccggtgagc gggcgcaaaa
     2641 tcgcggcgtt cagaaccccg aagaccacaa gattcgccac gatgcccatc gtgagcgaca
     2701 aaaccgcaac cagcacaaag ccaggcgcat gccgcagccg ccggacagcc acgcgaagat
     2761 caccccaaat gtcggacatt ggtctccttc acgaattcaa cagcccttca gagagaatgc
     2821 atctgcgggt ccatcccgcc accccgcgca gcgacgcaaa acagggcatt ttccctgaga
     2881 tgcaacgcgc gtctcgcgac cagcttgtcc agactggcgg atcggcgtcc attctcggac
     2941 aacgatcggc ctcgtgcagc gtcggaatca tacgggattg tcggagtcct gtaccctgca
     3001 ccctgtacgc tgtaccctgt cctcacccgc gatgctgcgc cgtctcgtcc tggctttttc
     3061 gttcctcctg gccgcgtgtc tgcctgccca ggccgctccg caccggcagg acaccggctt
     3121 tctgaaccgg agtctggtcg tcaacggcac gctgcgccgt tatgtcgtct acctgcccga
     3181 cacctggaac aagcagcagg cctggcccgt cattctcttt ctgcacggct ccggcgagcg
     3241 cggctccgaa ggcatggatg agacccaaat cggcctgccc cagtccatcc gtctgcatcc
     3301 ggaacgatgg ccgttcgtcg tagtcatgcc gcaggtgccg ttcagccacc atcactggac
     3361 cgatcccgac atgatgacca tggccatcgc cgctctcgat gcggaggtga aggaattcca
     3421 cggcgatcgc gaccgcgtct acctcaccgg cctctccctc ggcggatacg gcgtctggga
     3481 gatcgcgaag gcgtatcccc accgcttcgc agcccttgtg ccggtctgcg gtggcgtctt
     3541 ctggtcgtat cagcccgatc gctggcgcga ggtgaacacg ctgccggctg aatacgcccg
     3601 cttcgtcggt cgcacgccca cctggatctt tcatggcgcc gacgatcctg tggttaatcc
     3661 gcggcagtcg cggctcatgt ttgcggctct gaaggccagc ggcggcgatg tgcgcctctg
     3721 ggaatacgct gggatcaaac acaacgtctg ggaccgcgcc tacggcgaac cgcagttgcc
     3781 gcactggctg ctggcgcagc ggctcagcgc gatcccaaaa ctttctccgc tcgccgagga
     3841 gatcctggtt ccggtccatc cggtcccggc caaagtggat ccgaacctct acaacaccta
     3901 caccggagaa tacgaagacc agaacgaagt cgtcgccacg gtcttccgcc agggcgaccg
     3961 tttgttctcc cgcaatcgca tcggcgatgt gaacgagttg ctgccggaaa gcccaaccac
     4021 cttcttctac ccgaccggag gaacccggcg tctgaccttt gagcgcgacg ccgaaggccg
     4081 aatcaccgga atccgcttcc gggacgaccg acacgaggag ttctgggagc gcaagcgctg
     4141 acgagcgccg gccaagcgga gcgccgactt tttggacaaa agttttgaca tcgcctcgaa
     4201 ctcctctcgc cgactccgcg gcaagtccca caaatcgcac gacttcactt tggccgccgg
     4261 cgtgcgctta cttctcccga tgaaaccctg ctttccggca gaggaatctg ttcaaacgaa
     4321 tgaggagaag atcatgaagg gcttgctcta tgcggcagca atcctggcgc tgctttcggt
     4381 tcccgccttt gccggcgcaa aaacccagcg tcaaaccatc actttttcga acccggtcac
     4441 cgtcggaacc gcgaacttcg ttcccggaga ctgcaaagtg agctgggacg acaccggcga
     4501 caccgtgcag gtcaccttga cgcaaaaggc caacgatgct tcggtcaccg tgcccgccaa
     4561 agtcgtcaag ggagactttc cgcaccgttc gctcgtcttg acgaccgggg acggcgggca
     4621 ggttctcacc gcgatccagt tcaaccacta cagccttgtg ctcaacggca cggagatggc
     4681 ctcggaaagc gcccctaaaa cggatcaata acacggctga cctggacgga gggtggctcg
     4741 gcatcgcgct gggcgccctc cttcggcatg cgcaggcgat ccccttttcg agcgatggga
     4801 ccaggattta tctttcggcc cgcctctggt gtggtttctc gcagattcgt tcaacagccg
     4861 atcctggttt tctataggac atgacttctc agcctgcgga aacactcgcc ttcggcacgg
     4921 aaaagtaaca gggcacgacc ttggtcgtgc cgcaaagcgt ccaaaaatga atcgggcttc
     4981 agcccctgag ccctgtctgc ggtcgggcgc cgaatggttc actgaaacgt cagcgatacc
     5041 ccccctcccc acacggtacg aacgtaatgg cggacatccc gctctccgaa tgaagacgga
     5101 tctccacgcc ccgccctggc gtccatctca gcaggttcat tttcccgaac cgctgaggtg
     5161 attgtctgtg aaaggcttct tcaaatattc gttgttcgct ttggttttca ctctcctggt
     5221 gactcccttg tttgccaaaa ctcacacggt caggatccag caggccgtaa cggtgggttc
     5281 gacgcagatt cccgccggtg actacaagct ttcctgggag ggaaccggcc ccatggtgaa
     5341 ggttacgctg gcacagcgag gcgccacgcc cgttgtcctc gatgcaaagc tggtgaccgg
     5401 taacaagaac g
//
LOCUS       DASZYN010000742         1991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_496872, whole genome shotgun sequence.
ACCESSION   DASZYN010000742 DASZYN010000000
VERSION     DASZYN010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496872"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="VHE33_19035"
     CDS             <1..1042
                     /locus_tag="VHE33_19035"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HVT99602.1"
                     /translation="YVSLVSGSASYRRLPEMLVQIEQGAQRVTGLDDRQTEETFETEF
                     TARSNPHRGYITIIEGCDKFCAYCVVPYTRGKERSRKSDSVLAEARRMADAGFTEIQL
                     LGQNVNSYRDPEGKRSFAELLAAVGEVVGIRRVRFTTSHPRDFTKEIVEAIDSVPSLC
                     DHVHLPVQSGSSKVLRLMAREYSREQYLERIAWIKGAKRTISMTTDIIAGFPGETVED
                     FEETLSLLSEVQYDGVFAFKYSPRPNTPALAMADSIPEEEKSKRLQVLLDRQREIQRS
                     NYERHVGEILETMVEGANPQRGQIVGRTSQNKTLNFTTTQPIAPAPGVYLPVRVTKAF
                     PNSLVGEAVQLQ"
     gene            1070..1543
                     /locus_tag="VHE33_19040"
     CDS             1070..1543
                     /locus_tag="VHE33_19040"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HVT99603.1"
                     /translation="MKIRGLMVDPSTNMPVVILKDPKSEAVLPIWVGLYEANAIQMEV
                     EKATTARPLTHDLLKNVVMGLNAQVQRVVVSELRGDTFYAVIWMQQNGEVVSIDARPS
                     DALALALRSDCPIFVSEEVLHAAKVLPAAESQPTEQDVRKWLENLGDEDLGKYKM"
     gene            1630..>1991
                     /locus_tag="VHE33_19045"
     CDS             1630..>1991
                     /locus_tag="VHE33_19045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99604.1"
                     /translation="MTFRSVVATERIVRTFDGGPPSLEPADLRRIRNFLIPQVHPFLG
                     AAVHETPLVEALRSAVPEANIVAIGSGIGAQVLRCHPGVSRIEPAPDPHRDFWGAVRA
                     YRNVARSFGGEPWCALFTG"
BASE COUNT          398 a          543 c          671 g          379 t
ORIGIN      
        1 gtatgtctcg ctggtatcgg gatcggcttc gtatcgacga ttgccggaga tgctggtgca
       61 gatcgagcag ggcgcgcaac gcgtgaccgg gctcgacgac cggcagaccg aggagacgtt
      121 cgagacggaa ttcacggcgc ggtcgaatcc acaccgtggc tacatcacga tcatcgaagg
      181 ctgcgacaag ttctgcgcgt actgcgtggt tccttacacg cggggcaagg aacgcagccg
      241 caagtccgac tcagtgctgg cggaagcgcg ccgcatggcc gatgccgggt ttacggagat
      301 tcaactgctg ggccagaatg tgaattcgta tcgcgatccg gaagggaagc ggagcttcgc
      361 agaactgctg gctgccgttg gggaagtcgt cggcatccgc cgcgtgcggt tcacgacctc
      421 tcacccgaga gatttcacga aggagatcgt cgaggccatc gactcggtgc cttccctgtg
      481 cgatcacgtg catctgccgg tgcagagcgg ctcatcgaag gtattgcgcc tgatggcgcg
      541 cgaatattcg cgcgagcagt atctggaacg catcgcatgg atcaaaggcg cgaagcggac
      601 gatcagcatg acgaccgata tcattgcggg attccctggg gaaacggtgg aggacttcga
      661 ggagacgctg tcgctgctga gcgaggttca gtacgacggc gtgttcgcct tcaagtattc
      721 accgcgtccg aatacgccgg cgctcgccat ggcggattcg attccggagg aggaaaagtc
      781 gaagcggctg caggtgctgc tcgatcggca acgcgagatc cagcgatcga attatgagag
      841 gcatgtcggg gaaattctgg aaacgatggt cgagggagcc aatcctcagc gcggacagat
      901 cgtgggacgg acgtcgcaga acaagacgct gaacttcacc acgacgcagc ccattgcgcc
      961 ggcgccgggg gtgtatctgc cggtgcgtgt gaccaaagcg tttccgaaca gtctggtggg
     1021 agaggcggtt cagcttcagt aggcagcgag ggacacgatg gaagttgaaa tgaaaatccg
     1081 aggtttgatg gtcgatccgt cgacgaacat gccagtcgtg atcctgaaag acccgaagag
     1141 cgaggcggtg ttgccgatct gggtcgggct ctatgaggca aacgccatcc agatggaagt
     1201 ggagaaggcg acgacggcgc ggccgctgac gcacgatctg ctgaagaacg tggtgatggg
     1261 actgaacgcc caggtacagc gggtggtcgt ctcggaattg cgcggagaca ccttttacgc
     1321 ggtgatctgg atgcagcaga acggagaagt tgtgtcgatc gatgcgcggc cgtctgacgc
     1381 gctggctttg gcgctgcgct ccgattgtcc catcttcgtc tccgaagagg tgctgcatgc
     1441 cgccaaggtc cttcctgcgg ccgaatctca gcctaccgag caggatgtgc ggaagtggct
     1501 ggaaaacctc ggcgatgagg atttaggcaa gtacaagatg tgagcagggg ccggcgctga
     1561 agccgccccc ctgagccgcg cctgctggcc gagatcacgg gagaaagttt gatcgacctc
     1621 atcaaacgga tgacattccg tagcgtggtt gccacggaac gaatcgtccg aacttttgac
     1681 gggggtcctc cgtcgctgga gccggccgat ctgcgcagga tccggaattt cctgatcccg
     1741 caggttcatc cgttcctcgg cgccgcagtt cacgaaactc cactcgtcga agcccttcgg
     1801 tccgctgtgc ctgaggcgaa tatcgtcgct atcggatcgg gaatcggcgc ccaggttctt
     1861 cgctgtcacc cgggggtgag ccgtatcgag cctgcgccgg atccccaccg agacttttgg
     1921 ggagcggttc gggcttatcg caatgtcgcg cgctccttcg gtggtgagcc gtggtgcgcg
     1981 ctgttcacgg g
//
LOCUS       DASZYN010000743         3601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_496908, whole genome shotgun sequence.
ACCESSION   DASZYN010000743 DASZYN010000000
VERSION     DASZYN010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496908"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VHE33_19050"
     CDS             complement(<1..263)
                     /locus_tag="VHE33_19050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99605.1"
                     /translation="MHTLRPIRHRTPLLFGCAALALIVAPLASAATLCVQKQGLLGCQ
                     KTIAAAVSAAAPGDVIYVAPGVYKESVTITQSVSLVALPGTHTV"
     gene            746..3397
                     /gene="gyrB"
                     /locus_tag="VHE33_19055"
     CDS             746..3397
                     /gene="gyrB"
                     /locus_tag="VHE33_19055"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HVT99606.1"
                     /translation="MATKEVAPTEEAIPANVKAKGGKSAGENGNGSYTGENIKVLEGL
                     EAVRLRPAMYIGSTGEMGLHHLVYEVVDNSVDEALAGYATKIEVAIHDDNSITVIDDG
                     RGIPVDEMTLDTGEKMPAVQVVLTKLHAGGKFDTSNYKVSGGLHGVGVSCVNALSEEF
                     EVEIWRPAKPDEPSHTYEQSYSKGAPTTKLKQTGTSKRRGTKIRFLPDRSIFTTTEYN
                     YDTLAQRLRELAFLNKGLTITLTDERITDPKTGEAKKAEFKYAGGIAEFIKHLNRGKS
                     VLHDKPIVMEGLRDGVDMEIALQYNDGYSETVFCFANNINTVDGGTHLSGFRTALTRT
                     INYIGQQMGLFKDVKENLTGDDVREGLVAVISVKLPQPQFEGQTKGKLNSDIAGTVQA
                     LVNERLGAFLEQNPPVARKIINKAVEAARAREAARKARDLTRRKGALDGGGLPGKLAD
                     CSERDPGRCELYLVEGESAGGTAKQGRDRRFQAILPLKGKILNVEKARYDKMLGHEEI
                     RAMITALGTGIGKDDFDAAKLRYGKIILMTDADVDGSHIRTLLLTFFFRHMQELIKRS
                     NVYIAQPPLYKIKKGRFEQYIKDDRDFVKVMVKRAAEGMIIRYGEGAAKIEGAALTKF
                     MGQLNEYLGFYDKVNTRLRNEEVTNLLAHHELTKRADFENKTKLDALHKDLEKIQKKH
                     QFKALQKPVQDEEHQTWSLCFTDAQGAERRIDWALASTPEYRQMMSKHGQIKEMLEPP
                     FVVEYAGKAAEKAGVGVPDEEDLGAEAVAETKGEAEAGKPAPKRTGKANLEPVEKQTP
                     RELFEYVIEQGRRDYQVQRYKGLGEMTASQLWETTMDPERRTLLSVKLEDIAETETIF
                     TTLMGEDVDARRKFIEDNALDVKNLDI"
BASE COUNT          848 a          877 c         1300 g          576 t
ORIGIN      
        1 acggtgtgcg tgccgggaag ggcgaccagc gagacggatt gggtgatggt gacgctttct
       61 ttgtagacgc caggtgcgac gtagatgaca tcgccgggag cggcagcgga gacggcggcg
      121 gcgatggttt tttggcagcc cagcaggccc tgtttctgga cacagagggt tgcggcagag
      181 gccagaggtg cgacgatgag cgcgagtgca gcacagccga acagcaaggg cgtgcgatga
      241 cgaatcggac gtagggtgtg catgggacct ccagggattt ccgaattgcg tcttcggcgc
      301 ggcggggagg tgcgagtgga ccgggctcgg ggacgacggg ggccatgcta cacccgggaa
      361 atgccggcga atatgactgc gtcaacaact gtcgaaagat ggggtgttga tcgtgaaatt
      421 ggtggacgga tttgaactgc gggaccgggc ccgagtcctg acttcgggcg gctgaagccg
      481 gcggttgcgg aagagagttc tcatggcgtg acattgcgat ttcgccacct ccaggaggct
      541 ctgcaggacg cccgatgctc cggccgtcag aaaacccgct tttgcactga ttttgcacgg
      601 gttttgggcg gctggagagc ggtcttagcg tggtaaaata aggatggaag cggagcccgc
      661 aaatcctcgt ttttacaggg cttttcgggc ccatcacgag ccttctcacc cctggaaaaa
      721 tcaccccaga caagaccgga gtttcatggc aaccaaagaa gtggcaccca ccgaagaagc
      781 gatcccagcg aacgtaaaag caaagggcgg caagagcgcg ggcgagaacg gcaacggttc
      841 ctacacaggc gagaacatca aggtgctcga ggggctggag gcggtgcgtc tccggcctgc
      901 gatgtatatc gggtccacgg gggagatggg gctgcaccac ctggtgtacg aggtagtgga
      961 caactcggtg gatgaggccc tggccggata cgccacgaag atcgaggtgg cgattcacga
     1021 cgataactcg atcaccgtaa tcgacgacgg acgtggaatt cctgtcgatg agatgacgct
     1081 ggacacgggc gagaagatgc cggcagtgca ggtggtgctg accaagctgc acgcgggggg
     1141 taagttcgac acctccaatt acaaagtgtc cggcgggctg cacggggtgg gtgtgagctg
     1201 cgtgaacgcg ctgagcgaag agttcgaggt ggagatctgg cggccggcga agccggatga
     1261 gccgagccac acgtacgagc agagctactc gaagggcgct ccgacgacga agctgaagca
     1321 gacggggacc agcaagcggc gcgggacgaa gatccgcttt ctgccggacc ggtcgatttt
     1381 taccacgacg gaatacaact acgacacgct ggcgcagcgg ttgcgggagt tggcgtttct
     1441 gaataaaggg ctgacgatca cgctgaccga cgaacggatc accgatccga agaccggcga
     1501 ggcaaaaaag gcggagttca aatacgcagg cggaattgcg gagttcatca agcacctgaa
     1561 ccgcggcaag agcgtgctcc acgacaagcc gatcgtgatg gaggggctgc gcgatggcgt
     1621 ggatatggag atcgctctgc agtacaacga tggctattcg gaaacagtgt tctgcttcgc
     1681 aaacaacatc aataccgtgg atggcgggac gcatctttcc gggttcagaa cagcgttgac
     1741 gcggacaatc aactacatcg gccagcagat ggggctgttc aaagatgtga aggaaaacct
     1801 gacaggcgat gacgtgcgcg aggggctggt ggcggtgatc agcgtgaagc tgccgcagcc
     1861 gcagttcgaa gggcagacga agggcaagct gaactccgat atcgcaggca cggtgcaggc
     1921 gctggtgaat gaacggctgg gtgcgttcct ggaacagaat ccgccggtgg cgaggaagat
     1981 catcaacaag gcggtggaag cggcgcgtgc tcgcgaagca gcgaggaaag ctcgggatct
     2041 gactcggcgc aagggtgcgc tggatggcgg tggactgccc ggcaaactgg cggactgctc
     2101 ggagcgcgat cctggacggt gcgagctgta tctggtcgag ggcgagagcg caggcggaac
     2161 ggcgaagcag gggcgcgacc ggcggttcca ggcgattctg ccgctgaagg gcaagattct
     2221 gaacgtggag aaggcgcgct acgacaagat gctcgggcac gaggaaatcc gcgccatgat
     2281 tacggctctg gggaccggca tcgggaaaga cgatttcgac gcggcgaagc tgcgttacgg
     2341 caagatcatt ctgatgacgg acgccgatgt ggacgggtcg cacatccgca cgctgctgct
     2401 gacctttttc ttccggcata tgcaggagct gatcaagcgg agcaacgtgt atatcgcgca
     2461 gccgccgctg tacaagatca agaaggggcg gttcgagcag tacatcaagg acgaccgcga
     2521 ctttgtgaag gtaatggtga agcgcgcggc ggagggaatg atcatccgct acggcgaggg
     2581 cgcggcgaag atcgaaggcg cagcgctgac gaagttcatg gggcagctga acgagtacct
     2641 cgggttctac gacaaggtga acacgcggct gcgcaatgaa gaggtcacga atctgctggc
     2701 gcaccatgaa ctgacgaagc gcgcggactt cgagaacaag accaagctgg atgcgctgca
     2761 caaggatctg gagaagattc agaagaagca ccagttcaag gcgctgcaaa agccggtgca
     2821 ggacgaggag catcagacct ggtcgctgtg cttcacggat gcgcagggtg cagagcggcg
     2881 gattgactgg gcgctggcgt cgacgccgga atatcggcag atgatgtcga agcacgggca
     2941 gattaaggag atgctggaac caccattcgt cgtcgaatac gcgggcaagg cagcggagaa
     3001 ggcaggggtg ggggttccgg acgaggaaga cctgggcgcc gaggccgtgg ccgagacgaa
     3061 gggcgaagcg gaagcgggca aaccagctcc gaagcgcaca ggaaaagcaa atctggagcc
     3121 ggtggagaag cagaccccgc gcgagctctt tgagtacgtc atcgagcagg ggcggcgcga
     3181 ttaccaggtt cagcgctaca agggcctggg cgagatgacg gcgtcgcagc tatgggagac
     3241 gacgatggat ccggagcggc gcacgctgct gagcgtgaag ctggaagata ttgcggagac
     3301 agagacgatc ttcacaacgc tgatgggcga ggacgtggac gcgcggcgga agttcatcga
     3361 agacaatgcg ctggatgtga agaacctgga tatctgaaga ccggacccgc agggagcagg
     3421 gaatagggag tagggaatag aaaagacagt ggccctcctg aatggagggc tattgtcttt
     3481 ttagaggcga gcagaaatgg atacgaaaga gattggcaaa attctcggag agaagtttga
     3541 aggcgttgcg ggagatggcg cgctggcgtt agccgagctt catctggcgc ccgatgcggc
     3601 g
//
LOCUS       DASZYN010000744         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_303334, whole genome shotgun sequence.
ACCESSION   DASZYN010000744 DASZYN010000000
VERSION     DASZYN010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303334"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            705..2180
                     /locus_tag="VHE33_19060"
     CDS             705..2180
                     /locus_tag="VHE33_19060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99607.1"
                     /translation="MLGSRMGHKVKEHEQLEELHARIATKINQFNSFGLTVHGRAMLT
                     NSCLLSHLWFAASFLFMGDDFLNRLRSECYRFIMAGSPGYRHESRARCEARRGVGGLG
                     VLNPLVEVRAIRAMWVVRMLRGDAEDAATPRPWRQLLWTSLLRLANQRTTDRFNIFRK
                     DWPSFPFYDPALPKPLATAKGPAYHSLLTEILRDFSRCQPEHAPPPPRVAWAFSPTAG
                     SFKWVTIPPLPTAGDLDNLRLCNGKKLEELSVKKIYKFLLAQDRQAQADADHLKWEKR
                     ATKRAAAGLTPEPEPLRIDPDSPIPPRAWLEDKDPASWKRRWLALKQLRMPTTYKQLL
                     WRRWHGRLYLGATKEQPQPECPLCGELDSQEHFGRGCEVAGLLSLLQSLWRHWFDAPF
                     PEEWWVLEWGADWRWNTLFATALHTLYCLRYGVIRGDCSFSRIGLALRIKANLTAILD
                     VLARQPNGLIDHDGKWIRLKAGEDPANEKARREVCISFPFP"
BASE COUNT          425 a          799 c          735 g          539 t
ORIGIN      
        1 tggagcccgt gcgcgggctt tggtgcaagc acaattgacc gcgcctccaa tttggagccc
       61 tctggctctg ccccggcccg gaccggcctg ctggaatggc ctggcagccg atcccgcgcc
      121 cttggaccca cttgcctgcc tcctcggact tcgctcggcg gctggtgcgc ggtgcctctc
      181 ctcccgattc ctgtctcctt gcagcgccac gctttctctg ttcctgctct ttgatgggat
      241 agagaaaacg gacgatgtaa tgtgagaaga taatcggatg gatgcacctg cgtcgacgcc
      301 tttcgaagtc cttggaggag ttcattctgt ttatttctcc ggtcgtccca gtctagttct
      361 tcgaccttaa cttcgtcagc gtcggccgct atgcaggagc agctttgccg ccacgtggag
      421 tgtgctgcgg aattttctgt ggaactttcc acggaatttt ctgacgacga ccggccgcgg
      481 cgcatgcaga ctgcgggatg cgcatttctg ttcgagcagt agtgatggat tgtctgtcgg
      541 gggagagcat tttctctctt tgtgttggag gactttatgt cgtttgatgt cgccatcggc
      601 gattcatcag accctgtcac tccctacggg aggagtcttt gtcacgtgcc tacgggcgcc
      661 tcaaataaaa tgcacttgaa ttgaattgaa ttgagttcga acgcgtgctg gggtcgcgca
      721 tgggccacaa ggtgaaggag cacgagcaac tcgaggagct gcacgcgcgc atcgcgacca
      781 agatcaatca gttcaactct ttcggcctca cggtccacgg ccgggccatg ctcaccaact
      841 cgtgcctgct ctcacacctg tggttcgcgg cctccttcct cttcatgggc gacgacttcc
      901 tgaaccgcct gcgctctgag tgctaccgct tcatcatggc gggctcgccc ggctaccgcc
      961 acgaaagccg ggcccgctgc gaggcccgtc gcggagtggg aggtctcggc gtgctcaacc
     1021 ctttggtgga ggtgcgggcc atccgcgcca tgtgggtcgt ccgcatgctc cgcggcgacg
     1081 ccgaagacgc ggccaccccg cggccttggc gccaactgct ttggacctcg ctgctgaggt
     1141 tggccaacca gcgcaccacg gaccgcttca acatctttcg caaggactgg ccctccttcc
     1201 cgttctacga tcctgccctg cccaagccac tcgctaccgc gaagggccct gcctaccatt
     1261 cgctgctgac ggagatcctg cgcgacttca gccggtgcca gccagagcac gcgccacccc
     1321 cgccgcgagt ggcgtgggcc ttcagcccca ccgctggctc cttcaagtgg gtgactatcc
     1381 cccccttgcc cacggctggg gatctggaca atctgcgtct gtgcaacggc aagaagctgg
     1441 aagagctctc tgtcaagaag atctacaagt tcctcctggc tcaagaccga caagcccaag
     1501 ccgatgccga ccatctcaag tgggagaagc gcgcgacgaa gcgcgctgcg gcaggcctca
     1561 cccccgagcc cgaacccctg cgcatcgacc cggatagccc catcccaccg cgggcctggt
     1621 tggaggacaa ggaccccgca agctggaaga ggcgctggct ggcgctgaaa cagcttcgca
     1681 tgcccacgac ctacaagcag ttgctctggc gccgttggca tgggcgtctc tatctgggtg
     1741 ctacgaagga gcaaccccaa cccgagtgcc ccctctgcgg cgagctcgat tctcaggagc
     1801 actttgggcg cggctgcgag gtggctggtc ttctctctct cctccagtct ctgtggcgtc
     1861 actggttcga cgcccctttc cccgaggagt ggtgggtgct cgagtggggc gccgactggc
     1921 gctggaacac cctcttcgcg accgccctgc acactctgta ctgcctgcga tacggcgtga
     1981 tccgtggcga ctgctctttc tcgcggatcg gtttggctct tcgcattaag gccaacctca
     2041 ccgctattct cgacgttctg gctcgtcagc cgaacggcct aatcgaccac gacggcaagt
     2101 ggattcggct taaggctggc gaggaccctg cgaatgagaa ggcgagaagg gaggtctgca
     2161 tctccttccc cttcccttga ggtgaccgcc tctggcggta ttgttactta tttatgctgc
     2221 tgaggcccgt tcgggggctc tctatggact aagcgagcag ctctgtacag tgtatttaag
     2281 ctgtcttgta atctggcggt cctggctaac gcctggtgac caataaaggc tcaaaaagca
     2341 aaagcaaaag cactggaacc acgtgaagcc tccgcggtcc tggtggacga gcgagtggtc
     2401 tcctgattgg cggtggatgg ttggctactc gctcctactg cacgctctgt acgctcatcg
     2461 cgtggccgca gtccacgagg aggtggccgc cgacaacg
//
LOCUS       DASZYN010000745         2252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_954926, whole genome shotgun sequence.
ACCESSION   DASZYN010000745 DASZYN010000000
VERSION     DASZYN010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954926"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(636..1964)
                     /locus_tag="VHE33_19065"
     CDS             complement(636..1964)
                     /locus_tag="VHE33_19065"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HVT99608.1"
                     /translation="MTQRDRDRLVVLKKAQKRLITQSQAAKDLELSERHIRRLLVRLH
                     ESGDKSVIHGLRGRESNRKLSTEKREKAIRILSTEVYRGFGPTLASEYLAKKHKLAIG
                     RETLRQLMRQAGLWRSRKQKVEAVHQWRQRRSSRGEMVQWDTSDHDWLEGRGERFYLI
                     HMIDDATSELLARFVSRDSTEENMRLLWTYLERHGRPLSFYTDKASMFHNTPKVLRNQ
                     KNLSRDERDPLPPTQIGRALRELNIVWIAAHSPQAKGRVERSFGTAQDRLVKGLRVAG
                     ACTLDEANHYLETEFLPWWNQHLVVAPVTAADAHRPLPPDCDLTAVLSIVETRQVNND
                     YTIQFQGQFYRIASSSIVAGLRGAPVRVEKRLNGEICVRFRERYLEVAAIEQRTEPAE
                     PTPSPRSRKAKHPGPSPAMRAVMDNLLRKRGMPVWAAGKINNNHASEPRD"
BASE COUNT          410 a          684 c          660 g          498 t
ORIGIN      
        1 gtaaccacga aaatgcggcc atgcttcgga ggtaaaaatt caggaaggcg cgcaatcgcg
       61 cctcgctcga ccaccgcggt atacgtgcgc tgcggcgtcg cgacagaaaa ctggggcatt
      121 tcctcaatcg tcgcatgccg ctcgcgcctc agcgtctcgc gcctgccgcg aatgtttgtg
      181 atgcggaaac cagaaaattt tcctgcatat tgaagcacaa ttaccgtcac ggcgctgccg
      241 gtgaatcatt ttccggataa atcggggtaa ctgctgtttc cgtgccgtaa gtttgcgagc
      301 tgtcaatgcc aaatagaaat gtcctgatgt ctgccaggta gaattgtccg gtttcgagga
      361 tgagtagccg gaagaggggt taaggtggac gtgtccggac ggggtcgcgc ccgcacggat
      421 gcccaaagcc gaagcgacgg agctggctcc ggaagcagtc ggcgcaagcc ctcccagcgc
      481 tcgatgacaa ccgggctgac cggcccgctg cgcgcggagg gctttcctgt agaggacgaa
      541 caactgcttc tggtgaagct cccgagccga ggctttgggc tgaataaacg cgaaagttcg
      601 ccgggccggt tttgcccggc gttccttgct gcctttcaat cccgcggttc ggaagcgtgg
      661 ttgttgttga tcttgcctgc cgcccatacc ggcattccgc gtttgcgcag aagattgtcc
      721 atcacggccc gcatggctgg cgatggacct ggatgtttgg ctttccggga acggggcgat
      781 ggagttggct cggccggctc tgttcgctgt tcgatggcgg cgacctccag ataacgctcg
      841 cgaaaacgca cacagatctc tccgttgagc cgcttctcga cgcgtaccgg cgcgccgcgt
      901 agtccggcga cgatcgacga actcgcgatc cggtagaact gcccctggaa ctggatcgtg
      961 taatcgttgt tcacctggcg ggtttccact atcgagagca ccgccgtcag atcgcaatcc
     1021 ggcgggagcg gccggtgcgc atcggcagcc gtgaccggag cgaccaccag atgctgattc
     1081 caccagggca gaaactcggt ttccagatag tgattggctt cgtccagtgt gcaggctccc
     1141 gccacgcgca gccccttgac cagccggtcc tgcgccgtgc cgaagctccg ttcgacgcgc
     1201 cctttggcct gcggtgaatg cgccgcgatc cagacaatgt tcagttcccg caacgcacgg
     1261 ccgatctggg tggggggcaa cggatcgcgt tcgtcgcggg acaggttttt ctgattgcgc
     1321 aagaccttcg gcgtgttgtg aaacatactg gctttgtctg tatagaagct cagcggcctg
     1381 ccatggcgct ccagatacgt ccagagcagc cgcatgtttt cttccgtcga atcgcgggac
     1441 acgaagcgag ccagcagttc gctggtggcg tcgtcgatca tatggatcag atagaaacgc
     1501 tcgccacggc cctccagcca atcgtggtcg gaagtgtccc actgcaccat ctcgccgcgc
     1561 gagctgcgcc gctgccgcca ttgatgaacc gcttccactt tctgcttgcg gctccgccat
     1621 agcccggcct gacgcatcaa ctggcgcagg gtttcgcgtc cgatcgcgag cttgtgcttc
     1681 ttcgccagat actcgctcgc cagcgtgggt ccgaatccgc gatagacctc cgtggaaagg
     1741 atgcgaatcg ccttctcccg cttctccgta ctgagtttgc ggttggattc gcgtccccgc
     1801 aggccgtgaa tcacgctttt gtcgccgctt tcgtgaagac gtaccagcag acgccggatg
     1861 tgcctttcgc tcaattccag atccttcgcc gcctggctct gcgtaatcag tctcttctgt
     1921 gctttcttca gcactaccag ccggtctcgg tcccgctggg tcatggtgag aactccttgt
     1981 tctgacacct ccccagcctg acctcgtcaa caggacattt ctatttggca ctaataggac
     2041 attctcactt tgcgccgaca agaaaatttt cggtattgta ccgatccggg gtgcgagtta
     2101 cactcagcta ccacatgtcg aattcaccag gccggatatg tattttcgtc cttcgacggc
     2161 tcgctttgcc ggtagtggca ctcttgattg cgtcaggtgc ggcatcaact ccagccagcc
     2221 gggaaagcgg ggttagctcg tcaggcacaa cg
//
LOCUS       DASZYN010000746         4349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_372279, whole genome shotgun sequence.
ACCESSION   DASZYN010000746 DASZYN010000000
VERSION     DASZYN010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372279"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..613)
                     /locus_tag="VHE33_19070"
     CDS             complement(263..613)
                     /locus_tag="VHE33_19070"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HVT99609.1"
                     /translation="MQAETKTTVDQTSGASGAPVSVLRFSGDISSTSKDSLLGAWQSV
                     HDGPVLLDFTKVDYINSSGIALIIQMLMEADKTGRKVAAFGLTPHFQKVFTMVGIAKY
                     AGLYKDEPTAAASL"
     gene            complement(613..1077)
                     /locus_tag="VHE33_19075"
     CDS             complement(613..1077)
                     /locus_tag="VHE33_19075"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT99610.1"
                     /translation="MIPTAGTSVEVRLPSRLGFEKVAMGTAASVAQLMGFPPDRIEDL
                     KTAVAEACINAIEHGNRLNEKLSVGVILSATADGLEVKVVDNGTGMEKQPPKPDIDRK
                     IHGEEDPRGMGMFLIQALVDEAEWVHDSKGKGSYVRLVIRLDKDSEFDKESK"
     gene            complement(1091..2236)
                     /locus_tag="VHE33_19080"
     CDS             complement(1091..2236)
                     /locus_tag="VHE33_19080"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HVT99611.1"
                     /translation="MATPMPSAPSVDTLDLQQEIVRLQALLETSRQVHATIRLEDVLS
                     TILRIVVRELELPGALNTEPRIAYGELPPEPWEGCFRFPLNDKDGNRLTELIIATPDC
                     RPLTLYEEDFLERLALQAAVAVENARYYERSVAWARVEQDLHAARAIQRSLVPQEMPE
                     IAGYSVAVRYSACYEVGGDCVDIVTLPDGVQVMAVADVAGKGLPSAIVGTAFRSAFRA
                     SVGAGLPLADLVSRLNRQHCAENDETRRRYVTGIFLRLDPRSHQLEVVNAGHNPGFLV
                     RPGKAAHQLQASSTPIGILPIAEYQSEFVDFPPGSRLLFYTDGLTEVFCGDEEFGTER
                     LLNSFLQCTSPECAAILDTLFQELRSFSGNESQEDDMTALALMRMSA"
     gene            complement(2439..3992)
                     /locus_tag="VHE33_19085"
     CDS             complement(2439..3992)
                     /locus_tag="VHE33_19085"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HVT99612.1"
                     /translation="MRIGIDFGTTRVVVASVDRGNFPLVNFQTPDGQACDWFPPVVAV
                     RGDTRLYGWQAVSVLGDDSWTVLRSLKRALRDASPHTELRAGGRFVPLRQLLTEMMAA
                     LRTQLLEHSNLGAKRGEPLEAVLGVPANANSNQRFLTEEAAQNAGFTVIGLMNEPSAA
                     AIEFAYRNSAERHSRAGSSLLVYDMGGGTFDVSLVTLGETEHTVDASDGISNLGGDDF
                     DQILSDLAMETAGLDVELTGPEWFRLREECREKKESLNPQTRKVAIDLDRVRSGWPQV
                     TVPVDTFYERCRPLIESTREIVESILASHPDLNLDTLYVTGGGSELPPVARILRESFG
                     RKVRRSAYMRSASAVGLAIRASAQADGLRDQFNRNFGIWREADAGATIVFDLIFPRGV
                     RLPQPGEPALSSERVYHPAHNVGHFRYLECSTLDDRGQPHGEITNWEQIRFPYDPHLQ
                     NNADISHLPVERFSDPINLTIREKYTCDADGNLHVNISADPTGFAREFLLGQIEAGSP
                     APQTATTRN"
     gene            4161..>4349
                     /locus_tag="VHE33_19090"
     CDS             4161..>4349
                     /locus_tag="VHE33_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99613.1"
                     /translation="MAAKQLLPALGGSSAVWITCLVFFQTALLLGYLYAHWLGKLSSA
                     RAATVVHLALLVAAAGLLI"
BASE COUNT          780 a         1072 c         1661 g          836 t
ORIGIN      
        1 agctgttttc gattgtgttg ttccggacgc tgaagcgggc ggtggagggg cggccgttca
       61 actgggagaa gattgagcgg acggcgcgaa tgtcgcggca gacggagaag attgcggcag
      121 gggactgagc gagaccggga acaggggagt cagccgggat ccatttggaa aaagacccgc
      181 tcaggaggac ggagcagatt cttttccaaa gcaactggag cggtcaggtg atcggcggag
      241 ctcgcttgtc ccgggccgat gcttaaaggg aagcagcggc ggtgggctca tccttatata
      301 agccggcata tttggcgatg ccgaccatgg tgaatacttt ctggaagtgc ggggtgaggc
      361 cgaaggccgc gactttgcgg ccagttttgt cagcttccat gagcatctgg atgatgaggg
      421 cgatcccgct ggagttgatg tagtcgactt tggtgaagtc gaggaggacg gggccgtcat
      481 ggacagactg ccaggcgccg aggagggagt ccttcgaggt gctggagatg tcgccgctga
      541 aacggagcac ggaaacgggc gcaccgctgg cgccggaggt ctggtctacg gtggttttgg
      601 tctcggcctg cactatttac tctccttgtc gaactcgctg tctttgtcga gccggatgac
      661 cagccgtaca tagctgccct tgcctttgct gtcgtgcacc cactcagcct cgtcgaccag
      721 agcctgaatg aggaacatgc ccatgccgcg agggtcttct tcaccgtgga tcttgcggtc
      781 gatgtcgggt ttgggcggct gcttttccat gccggtaccg ttgtcgacga ccttgacctc
      841 gaggccgtcg gcggtggcgg agaggatgac gccgacggag agcttctcgt tgaggcgatt
      901 gccgtgctcg atggcgttga tgcaggcttc agcgacggcg gtcttgaggt cctcgatgcg
      961 gtcgggaggg aagcccatga gctgagcgac gctggcggcg gtgcccatag cgactttttc
     1021 gaagccgagg cgcgagggga gccgaacttc caccgaagtg ccggcggtcg ggatcatcga
     1081 catcctccca tcaggcgctc atgcgcatca gagccagcgc ggtcatatcg tcttcctgcg
     1141 attcgtttcc cgagaagctg cgcagttcct ggaacagcgt atcgaggata gcagcacatt
     1201 ccggagaggt gcattgcaga aatgagttga gcaggcgctc ggtaccaaat tcttcatcgc
     1261 cgcaaaagac ctcggtgagg ccgtcggtat agaagagcag gcgggagccg ggagggaagt
     1321 ccacgaattc ggactgatac tcagcgatgg gcagaatgcc gatgggggtg gagctggcct
     1381 ggagctggtg ggcggctttg ccgggccgca cgaggaagcc gggattgtgg ccggcgttga
     1441 cgacttccag ctggtggctg cggggatcaa gccggagaaa gatgccggtg acgtagcggc
     1501 ggcgggtctc gtcgttttcg gcgcagtgct ggcgattgag gcgtgagacg aggtccgcaa
     1561 ggggaagtcc ggcaccgacg ctggcccgga aggcagagcg gaaggcggtg ccgacgatgg
     1621 ccgacggcag tcctttgccg gcgacgtcgg cgacggccat gacctggacg ccatcgggaa
     1681 gagtgacgat gtcgacgcag tcgccaccga cttcgtagca ggcgctgtag cgaacggcga
     1741 cggaatagcc ggcgatttcc ggcatctcct gtggaacaag gctgcgctgg atggcgcgag
     1801 cggcgtggag atcctgctcg acgcgggccc aggcgacgct gcgctcgtaa tagcgggcat
     1861 tttctacggc gacggcggcc tgaagggcga ggcgctcgag gaaatcttct tcgtagaggg
     1921 tgagaggccg gcagtcgggc gtagcaatga tcagctcggt gaggcggttg ccatctttat
     1981 cgttgagggg aaaacgaaag cagccctccc agggttcggg aggcaattca ccgtaggcga
     2041 tgcggggttc tgtattgagg gcgccgggca gctcgagttc gcgtacgacg atgcggagga
     2101 tggtggagag aacatcttca agacggatgg tggcgtgaac ctgacgagag gtttcgagca
     2161 gcgcctggag acggacgatc tcctgctgga gatcgagtgt gtccaccgat ggcgcggatg
     2221 gcatgggtgt ggccatgaaa cttgctcggg tacccgggga gtctggagca ggggaattgg
     2281 ttcactgtag cacggcgcat cgcttaggca caggtcgaag ggcaggatcc catgtctcaa
     2341 accagggata tggggcgccc ggcggaacag aagcaggttc ctccgctgcg ctgcgctgcg
     2401 gtcggaatga caagatcaat ggtctgctga agtagatatt agttgcgggt ggtggcggtt
     2461 tgaggggcgg gggagccagc ttcaatctgg ccgaggagaa attcacgagc gaaaccggtg
     2521 gggtccgcgg agatgttcac gtgcaggttt ccgtcggcgt cgcaggtgta tttttcgcgg
     2581 atggtgaggt tgatggggtc gctgaaccgc tccacgggaa ggtgggagat gtctgcattg
     2641 ttctgcagat ggggatcgta ggggaaacgg atctgctccc agttcgtgat ttcgccgtgg
     2701 ggctggccgc ggtcgtcgag ggtggagcac tcgagatagc ggaagtggcc gacgttgtga
     2761 gcggggtggt agacgcgctc gctcgaaaga gcaggctcgc cgggctgagg caggcgcacg
     2821 ccgcggggaa agatgagatc gaagacgatg gtggctccgg cgtcggcttc gcgccagata
     2881 ccgaagttgc gattgaactg atcgcggagg ccgtcagcct gggcggaggc gcggatggcg
     2941 agacccacgg cactggcgga acgcatataa gcagagcggc ggaccttgcg gccgaaactc
     3001 tcgcggagga tgcgcgcgac gggcggaagc tcggagccgc cgccggtgac gtagagcgtg
     3061 tcaaggttga gatccgggtg tgaggcgagg atgctctcga cgatttcgcg ggtggattcg
     3121 atgagggggc ggcaacgctc gtaaaaggtg tcgacgggga cggtgacctg gggccagccg
     3181 gaacgcacgc ggtcgaggtc gatggcgact ttgcgggttt gcggattgag ggattctttt
     3241 ttctcgcggc actcttcgcg gaggcggaac cattcgggtc cggtgagttc gacgtcgagg
     3301 ccggcggttt ccatggcgag gtcggaaagg atctgatcga agtcgtcgcc gccgaggttg
     3361 gagatgccgt cggaggcgtc gacggtgtgc tcggtttcgc cgagggtgac gagggagacg
     3421 tcgaaggtgc cgcctcccat gtcgtagacc aggaggctgg agccggcgcg gctgtggcgt
     3481 tcggcgctgt tgcggtaggc gaactcgatg gcggcggcgg aaggctcgtt catgaggccg
     3541 atgacggtga agccggcgtt ctgcgcggct tcttcggtga ggaagcgctg attgctgttg
     3601 gcgttggcag gaacgccgag aacggcttcc agcggttcgc cacgtttggc gccgaggttg
     3661 gagtgttcga ggagctgggt gcggagtgcg gccatcattt cggtgagcaa ctggcggaga
     3721 ggaacgaagc ggccaccggc gcggagctcg gtgtgggggc tggcgtcgcg aagcgcgcgc
     3781 ttgagggacc ggaggacggt ccagctatcg tcgccgagga cggaaacggc ttgccagccg
     3841 tagaggcgag tgtcgccgcg gacggcgacg acgggcggaa accagtcgca ggcctggccg
     3901 tcgggggttt gaaaattcac cagggggaag ttgcctcggt cgaccgaagc aacgacgacc
     3961 cgtgtcgttc cgaagtcgat gccgattctc atttgacttc agtttacggg tgaatcgcgt
     4021 ggaaaaacgg ggcggggcgg agggtggacg atgagcgtgg tagcgttctt atcggagcgg
     4081 atttggcgcg gcgcgcgatg cgaattttgt atggaagtgc aatttttctc ggggcgtttt
     4141 tgctgtttgc tgtggagccg atggcggcga aacagttgct gccggcgctg ggcgggtcgt
     4201 cggccgtatg gatcacgtgc ctggtgttct ttcagacggc gctgctgctg ggatatttgt
     4261 acgcgcactg gctggggaag ctctcgtccg cacgagcggc aacggttgtg catctggcgc
     4321 tgctggttgc ggctgcgggg ttgctgatc
//
LOCUS       DASZYN010000747         3985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_562208, whole genome shotgun sequence.
ACCESSION   DASZYN010000747 DASZYN010000000
VERSION     DASZYN010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562208"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="VHE33_19095"
     CDS             <1..1191
                     /locus_tag="VHE33_19095"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HVT99614.1"
                     /translation="VARILCDLKPEGLELSHIFNRSVARKKVDWVPNHVIWTEDFDQV
                     LRSDADVIVELVGGLEPAGGWVRRALEAGKSAVTGNKKLIAKHGIDLDRLARSKGAHL
                     LYGAAVAGGIPVIPGLLQGLAGDRITRIEAILNGTCNYILSRMEAGDAFADVLSDAQK
                     LGYAEADPSEDVDGYDARAKLVILSRIALRAAVEVESVGCRSITEVDAVDLVYARDLN
                     CTIRQISKAELRSDGVSAAVGPMLVPLRSPFAWSRSTENMVLVSGQYGGDVVFSGHGA
                     GGNPTAVAVVSDLISLAHGSRAVDLPSRATAVSGEFELRHYIRFVVRDKPGIVAEIAG
                     ALASEKINIDALFQRTGYQKGNLPFVVTVEPCAASALRRALDRIGNAAWLAQPPLDMP
                     ILGE"
     gene            1213..2391
                     /gene="hpnI"
                     /locus_tag="VHE33_19100"
     CDS             1213..2391
                     /gene="hpnI"
                     /locus_tag="VHE33_19100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HVT99615.1"
                     /translation="MFATIVAVVTALLTAAGLAYYLIALWSARAYLRRPRFPAGFAPP
                     VSILKPLHGLDPGMLDAFASHCQQTYSGEYELLFGVSRLDDPAAAVVQELQAMFPERA
                     IRLIQTPDNLGFNGKVSNVAQLLPHARFDFFVISDSDIRVGPHYLARVMAPFAPREGQ
                     RNVGLVTALYRGRAHRTVGSRLEALGISTDFAPGVLSSRFLERGIHFGLGSTLAVSRT
                     ALDTIGGLAPLADYLADDYELGARIDRSGFGVELATEAVETHVPAWSFSGYLRHQLRW
                     ARTMRDSRRGGYAGLLFSYGLAWSILNLVASGLSLPAFALFSLALMFRVALALGVGVG
                     ILDDRQVLRDLWLLPVRDMLALGVWAWSYAADTIAWRDERFVLRDGKLTQRETVGEKP
                     "
     gene            complement(2531..2794)
                     /locus_tag="VHE33_19105"
     CDS             complement(2531..2794)
                     /locus_tag="VHE33_19105"
                     /inference="COORDINATES: protein motif:HMM:NF003817.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HVT99616.1"
                     /translation="MVFCSRNKQEMEGLDEPPFDSDFGNKVYNNVSKKAWQEWIERQK
                     MLLNEYRLQPWTPQAQEFLVEQMDQFFFGEGAALPKEYVPPSH"
     gene            3195..>3985
                     /locus_tag="VHE33_19110"
     CDS             3195..>3985
                     /locus_tag="VHE33_19110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99617.1"
                     /translation="MKRTRFLTTALAAALFPVIGPSALHAQSAKEIVTTVVRNELDAD
                     TNDHTRWMYRDAYKSPDKDIVKIVIETAQGNLSEVIEDHGRPPSAQQHQDDLHQIQQM
                     VSDPALRARKKKAEQNDGQQARDMLNLLPKAFVWKIVDHSGGDIKLSFYPDPHFSPDS
                     MSGKVLAAMSGTLTVNESHMRLRELAGRLDHDVTFAWGLLGRINAGGTFHIVRKEVGD
                     GSWQITEMHVHISGHALFFKTIGDQEDEITSNYHRVPDGVDIGKAA"
BASE COUNT          714 a         1249 c         1215 g          807 t
ORIGIN      
        1 gtcgctcgca ttctctgcga tctcaagccc gaaggcctcg aactctctca cattttcaac
       61 cgtagtgtgg cgcgcaaaaa ggtcgactgg gttccgaatc acgtgatctg gaccgaggac
      121 ttcgaccagg tcctgcgctc cgatgcagat gtgatcgttg agctggtcgg cggcctcgag
      181 ccggcggggg gctgggttcg ccgcgcgctc gaagccggca aatccgcggt gaccggcaac
      241 aagaagctca tcgcgaagca cggcatcgac ctcgatcgtc tcgcccggtc caaaggcgcg
      301 cacctgcttt acggagctgc tgtggcgggc ggaatcccgg tgatccccgg gttgctgcag
      361 ggcctggccg gcgaccgcat cacgcgcatc gaggccattc tgaacggcac ctgcaactac
      421 atcctgagcc ggatggaagc cggcgacgcg ttcgccgatg tcctgagcga cgcgcaaaaa
      481 ctgggctacg ctgaagccga tcccagcgag gacgttgacg gctacgatgc gcgcgcgaag
      541 ctggtcatcc tgtcccgcat cgcgctgcgc gccgcggtgg aagtggaatc cgtcggctgc
      601 cgctctatta ccgaagtcga tgcggtggat ctggtctatg cacgcgatct gaactgcacc
      661 atccgccaga tctccaaagc ggagctgcgc tccgatggcg tatcggctgc cgttggtccc
      721 atgctggtgc cgctgcgctc gcccttcgcc tggtcgcgca gcaccgagaa catggttctg
      781 gtcagcgggc agtacggcgg ggacgtggtc ttttccgggc acggagccgg cgggaatccc
      841 accgcggtcg ccgtggtctc ggacctcatc tcgctggctc atggttcccg ggccgtcgat
      901 ctgcccagcc gcgccactgc ggttagcggt gagttcgagc tgcgtcacta catccgcttt
      961 gtggtgcgcg acaaacctgg catcgttgcc gagatcgccg gcgcacttgc ctcggaaaag
     1021 atcaacatcg acgcgctctt ccagcgcacc ggctaccaga agggcaatct gccgttcgtc
     1081 gttacggtgg aaccctgtgc tgcctcagcc ctgcgccgcg cgctcgaccg catcggcaac
     1141 gctgcgtggc tcgcacagcc gccgctcgac atgccgatcc tgggtgaatg actggggcga
     1201 atgaacaacc cgatgttcgc caccatcgtt gccgttgtca cggcgcttct tactgccgct
     1261 gggctcgctt attatttaat cgccctgtgg agcgcgcgcg cttatctgcg gcgtccacgc
     1321 ttccctgccg gatttgctcc gccggtcagc atcctgaagc ccctgcacgg gctcgacccg
     1381 ggcatgctgg acgcttttgc cagccactgt cagcagacct actccggcga gtatgagttg
     1441 ctgtttggcg tctcgcgcct cgacgatccg gcggccgccg tcgttcagga actccaggcg
     1501 atgtttccgg aacgcgcgat ccggctgatt cagacgcccg acaatcttgg tttcaacggc
     1561 aaggtgagca acgtcgccca gcttctgccg cacgcccgct tcgacttctt cgtcatcagc
     1621 gacagcgaca ttcgtgttgg cccccattat ctggctcgcg tgatggcgcc cttcgctccg
     1681 cgcgaagggc aacgaaatgt cggccttgtt accgcgctct atcggggccg cgcgcatcgc
     1741 accgtgggtt caaggctgga agcgcttggt atctcgacgg acttcgctcc tggcgtgctg
     1801 agttcgcgtt tcctcgaacg cggcatccat ttcggactgg gatcgacgct ggcggtctcg
     1861 cgcacggctc tcgacactat cggcggcctc gcgcctctgg ccgactacct cgccgatgac
     1921 tacgaacttg gcgcgcgcat cgaccgctcc ggcttcggcg tcgaactggc gacggaagcc
     1981 gtcgaaaccc acgttcctgc gtggagcttc tccggctacc tgcgccatca actgcgctgg
     2041 gcgcgcacga tgcgcgattc ccgccgcggc ggctacgcgg gtctgctgtt ctcttatggc
     2101 cttgcctggt cgatcctgaa ccttgttgcc tcaggattga gtctgcccgc gttcgcgctc
     2161 ttcagcctgg cgttgatgtt tcgggttgcg ctggcgcttg gcgtcggggt gggcattctc
     2221 gacgaccggc aggtactgcg cgacctgtgg ctcctgcccg tgcgcgatat gctcgcactc
     2281 ggcgtgtggg cgtggagcta cgccgcggac acgattgcgt ggcgcgatga acgatttgtg
     2341 ctgagggatg gaaagctgac tcagcgagag acggttggcg aaaagcctta gcgaaggacg
     2401 tttagcgggg tgctgttcgc caggtgctgt ttgcaaaaga cagtggccga ccagccagaa
     2461 cggtcgctag gcgtatttcg ctgaacgttc gctgctaaac gcactttgct aacgtcgttt
     2521 gctgcagccc tcaatgcgac gggggcacgt attccttcgg cagtgcggcg ccttcgccga
     2581 agaaaaactg gtccatctgt tcgaccagaa actcctgcgc ctggggagtc cagggctgca
     2641 ggcgatactc gttcagcagc attttctgcc gctcgatcca ttcctgccag gcctttttcg
     2701 acacattgtt gtagaccttg ttgccaaagt cgctgtcgaa gggcggctcg tccagccctt
     2761 ccatttcctg cttgttgcgc gagcaaaaaa ccatgtgcgg catcgtcggc atctctctga
     2821 gtattgtaca agtacgcgtt gagcttcgcg aggttgcggc cgttgcggcg gccacaactt
     2881 gtggagtgtc cctgaagttc attgaacact caggcgcccc acaggcaggc ctcaggggct
     2941 aaagcccgta ttattttggg cgttgtaggg cacgactgaa gtcgtgccct gatacaaaac
     3001 caggatcggt cgctgaacga atttgcgtga caccacacta gctagtattc cgatacgtcg
     3061 aacactgaag gatccggatt ccggtgagct gtgagagact ttgccttggc ctgcgcaccc
     3121 gcaaaacgat ataaggagtt cctgccgatg aggattccag cgtgctcccc agcccgtacc
     3181 ggggacgatc gttcatgaaa aggacccgat tcctgacaac ggctctggcg gccgccctct
     3241 tccctgttat cggtccatcc gcactccatg cccaatcggc caaagagatc gtcacaaccg
     3301 tcgtccggaa tgaactcgac gccgatacga acgatcacac gcgatggatg tatcgcgacg
     3361 cgtataagtc tcctgacaaa gacatcgtga agatcgtgat tgaaacggcg cagggcaatc
     3421 tctccgaggt gatcgaggat cacggcaggc cgcccagcgc ccagcagcat caggacgatc
     3481 tgcaccagat tcagcagatg gtcagcgatc ccgcgctgcg cgcaagaaag aagaaggcgg
     3541 agcagaacga tggccagcag gcgcgcgaca tgctcaacct gctgccgaag gcctttgtct
     3601 ggaagatcgt cgatcactcc ggcggcgaca tcaagctcag cttctacccc gatccccact
     3661 tctcaccgga cagcatgagc gggaaggttc tcgcggccat gtccggcacg ttgactgtga
     3721 acgaaagcca tatgcggctg agggagcttg cgggccggct cgatcacgac gtgacctttg
     3781 cctggggcct gctgggccgc atcaatgccg ggggaacgtt ccacatcgtc cgcaaggagg
     3841 taggggacgg cagctggcag atcacagaaa tgcacgttca catctccggc cacgcgctgt
     3901 tcttcaagac gatcggcgac caggaagacg agatcacgag caactatcat cgcgtcccgg
     3961 atggagtcga tatcggtaag gcggc
//
LOCUS       DASZYN010000748        22237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1203189, whole genome shotgun sequence.
ACCESSION   DASZYN010000748 DASZYN010000000
VERSION     DASZYN010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 22237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22237
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203189"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3557)
                     /locus_tag="VHE33_19115"
     CDS             complement(<1..3557)
                     /locus_tag="VHE33_19115"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HVT99618.1"
                     /translation="MKKLLFWQRTIVIIGLAAIPLAAAAVLLGFAPAASAQAISVNGG
                     SIQGTITDTSGAVIAGASVRILATDTGAVVTVTTDASGLYSVGPLNPGNYRVTISAAG
                     FETLVVNTVIRTATATPGTFQLKVGSQTQTVEVTAGAVQLNTDQPSVTGVISSQQFES
                     LPVNGRNVLDFAQLQPGVQLQAGGSSDGGFDPTKAGYSAVSFSGISGRTTRILLDGQD
                     VTDETVGTTIFNISQGSIGEEQITRATADPSTEVTSQGSVLLATKSGTNSFHGEAFEY
                     FQDQRAGVATYEDTSSPFQRNQFGGSVGGPILRDKLFFFANVERLKQDQSSPVSLGLN
                     FKAKQAEFPSVASPDRDTYSDGRIDWNGPHATHFFVRGNYERNSFATGAVYSTYSNLD
                     NAWGIAGGADFASSRFTNSFRGSFEKFHNQIGDTTLGNNSIYNPIPGFGIYYSAQGLH
                     TGPNENAPQQTFQSDKQLRYDGGWTRGSHNIRYGVSLNRILGGGLAAFFGYGPRASIN
                     SGTLIGDATDPIHGYKPYYVYISNNLGYASEKEGFGLPGGGQSDWRTGLYAVDTWKIN
                     PQFTFTWGLRWDRDTGRSNSDLAPVPCSNIATDNFAFGAGSSGLPCSGDSHLFDQWGA
                     GLGNRVSQPKWDFGPNIGIAYNPSFSQKTVLRGGFGIYYDSNVFNNVQFDRSSRLQNG
                     KFAVYTPVCYGGNYTLGDLTETSTGISIQALCNMSLADGAPGWLQLQKDYRAEVSGGG
                     VNSGSAAYNLAINNGAIAFAPDFKFPYSINISVGVQRELFSGAVISADYVRLETLRLG
                     QTIDANHVGDSRYFNLASAQAAAAATLTACQVGSVDAAITACPGLHPAKNGNPAGPAT
                     IADFAARGLDSGTSYTGGYPAAAYPKRIPAFPGVNPNVGVGTFQFPEGRASYNGLQVS
                     FMEQISHHLPGIRQSNFQASYAFSRMLSTAGDNGISAGSDPFFTSPSYNNRDTTADMG
                     WGGFDRTHILSFGGAATFKYGPQLSLIGHLSSARPTGLTIDDQGGGPGEIFHSDYNGD
                     GQIGDLMPTTKPGAYMRQYGPNSLNKLIDKYNAGYAGKPTPAGQTLITNNIFTGTQLA
                     ALGGVMPALDSAPSHAYPNSPLRTLDASLRYPIHFHWLPEKVNITPAVAMYNVLNFGN
                     FGGPDGVVLTPDDNGAGLTNVNSPYGTGAAAFDIKNTERT"
     gene            4048..4908
                     /locus_tag="VHE33_19120"
     CDS             4048..4908
                     /locus_tag="VHE33_19120"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HVT99619.1"
                     /translation="MNALKTAFLLTALTLLLVLIGSHWGENGAIFAFVIAAAMNFFSY
                     FYSDKLALAMYRAQPVTREQLPRAYEVVERMTGRLGLPMPKIYVIPTDAPNAFATGRN
                     PAHASVAVTHGILQLLNDDELEGVLAHELGHVRNRDILTSSIAATLAGAITLLARMGW
                     WAEMFGGFGGGGDRRRGGGISALFMLILAPIAAMMIQLWVSRTREYEADATGAHITGN
                     PYALASALEKLDAYSKRVPMPGTASTAHLFIVQPLLSREDFAGLFSTHPPIRKRIERL
                     IGRPSVYGPQ"
     gene            complement(4966..5958)
                     /locus_tag="VHE33_19125"
     CDS             complement(4966..5958)
                     /locus_tag="VHE33_19125"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HVT99620.1"
                     /translation="MTQRIPVFVFVAVTCFSAAASAQSLLVANQRDRTVSVIDAAQGR
                     EVATIAETVSDQWGHEITATADGHTAFLPIYGNSGVGKPGIDGSKMLVIDVASRKITG
                     TVDFGHPVRPHLPVLDAAHNRLYVTTELDNAITVIDPHTLKIVGKVPTGQKESHMLAL
                     SHDGRFGYTANVGPGTVSVLDLAGRKTLAVIPVATKVQRIAISQDDKYVFTSDVVEPR
                     LAVIDTATKKLKTWVPLPANGYGSAATRDGRWLLVAMPIANEVAVIDLQSMKVAREIP
                     VAASPQEVLIQPDGKFAYVSCMGAGQVAAIDLAQWKVAKLINAGKGDDGLGWAQ"
     gene            6071..6538
                     /locus_tag="VHE33_19130"
     CDS             6071..6538
                     /locus_tag="VHE33_19130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99621.1"
                     /translation="MLRTLVWLAIVVWFGGLLFFPITAWAAFSTIADTHQAGTIVAKC
                     LGVLHHEGLFAGCLIVFLLALGYFLKAFRMSTTSIGIAVTLLMIGCTVWSQYSIIPRM
                     ERDRIAAGGAIDSVPKTDPRHVDFDRLHNVSTDVEEAVMLGGLVLVVLLARDY"
     gene            complement(6571..6966)
                     /locus_tag="VHE33_19135"
     CDS             complement(6571..6966)
                     /locus_tag="VHE33_19135"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HVT99622.1"
                     /translation="MTADAQGFLSQRSQHSVEETVARLRSILDAKGVKLFALVDHSGE
                     AKKAGLEMRETKLLIFGNPAAGTPLMLAAPTIAIDLPLKILVWEDTDGAVWLSWNSPE
                     YLQQRHNLPSDLVKNISVVAALAAKAGGM"
     gene            complement(7362..7778)
                     /locus_tag="VHE33_19140"
     CDS             complement(7362..7778)
                     /locus_tag="VHE33_19140"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT99623.1"
                     /translation="MQTATNPAVHLHDIGQIALTVTNLDDARAFYRDILGMQFLFDAG
                     AMTFFQCGSVRLLLGTGENHAAPGGGTVLYFRVPDIETACASLREKGVGFSQEPHLVA
                     RMKSHDLWLAIFNDPAGNALALMSEIPRASTPEKTA"
     gene            7833..8273
                     /locus_tag="VHE33_19145"
     CDS             7833..8273
                     /locus_tag="VHE33_19145"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HVT99624.1"
                     /translation="MSTASDPAREELADSLHSAAIHLLRKLRRADVRSRLNAPRLSAL
                     SVIVFGGPLTLGQLAAAEQVRPPTMTRIVHALEARGLVKKNSNAEDRRTLHLSATTKG
                     RRVMHEARARRIHSLATQIEKLSRREQERLRDGLEIIGSLIREL"
     gene            8360..9241
                     /locus_tag="VHE33_19150"
     CDS             8360..9241
                     /locus_tag="VHE33_19150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVT99625.1"
                     /translation="MKLCIENVGKRYGSNVWALRRFSLDLKPGVLGLLGPNGAGKTTL
                     MNILATITRASEGTITWNGAEVAKDPNALRAVLGYLPQDFGVYPNLSAQEFLEYLAAV
                     KGLDATAARRRIDQLLNVVNLADVRGRPLGGFSGGMKQRVGIAQALLNDPRLLIVDEP
                     TAGLDPEERVRFRNLLSELSGERIVILSTHIVSDVEATATEIALISNGSLVAHASPEE
                     LLRRVDGRVWEWVIPSADLNAVKQAYKVSNTARRSDGVHVRILTEEPPDGAAPVPPTL
                     EDAYLDSLSVYRAGVAA"
     gene            9238..10842
                     /locus_tag="VHE33_19155"
     CDS             9238..10842
                     /locus_tag="VHE33_19155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99626.1"
                     /translation="MSSARVLWHLVKADFLTRVRRTSFLVTLGFAVFAGYQVFAGNVQ
                     MQLDDYRGVYNSAWVGALMGVVTSTLLTLVGFYIVKGSILRDETTHVGQILAATPMSR
                     GFYTVAKWLGNFSVLAAIVAVMGLAAVAMQLMRGEDRTLHLWTLLSPLILYALPAMAF
                     VAAMAVLFETLPLLRGGVGNVAWFFLWIGILVVGVGTSAEHGGAVLRGEYFRDFSGMP
                     TLMGQMRTTLVQVDPHFRNNFSLAIGGMKPHKQFLWEGVEWDAAQVTARLLWFVYALA
                     TALLAAIFFHRFDPGREGFARKRRTMQERAPVVELAAAKNLAQADPWTHRLSPLEPTA
                     PKNRFFALVAAELRVMLQGHRWWWYAIAAGLFVACAAAPVKASSGGVILAAWIWPLLI
                     WSKMGGRESRYATGALIFSAPHALDRQLPAAWVAGVLITLATGGGLLIRLLIAADGRA
                     VAGLLVAVCFIPSLALACGILTGGSKFFEALYTVGWYAGAAHHIRGLDFMGTVDASST
                     PAIYLALTVALLAVCWTRRRMQLAYA"
     gene            complement(10833..11717)
                     /locus_tag="VHE33_19160"
     CDS             complement(10833..11717)
                     /locus_tag="VHE33_19160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HVT99627.1"
                     /translation="MLHRIRITLEMIKWEHSIFALPFALTGAALAAHGFPPAAHAGWL
                     AAKLGWIVLCMISARSAAMAFNRWADADLDAANPRTRLRAIPAGLLARNFAAGFTLVM
                     SALFLFGAWRLNQLTLILSPVVLVILFLYSYTKRFTRWSHLFLGLALGIAPTGAWIAI
                     RGALDARILVLTLAVLFWVAGFDVLYACQDEQHDRTAGLYSIPATLGLASAFWIARGM
                     HLLMLALLIELAVLFHLGPIAAAGIVAVAALLLWEHTLVSPRDLTRLNAAFFTMNGVI
                     AVIFFLFVAADLLIRRHA"
     gene            complement(11868..12620)
                     /locus_tag="VHE33_19165"
     CDS             complement(11868..12620)
                     /locus_tag="VHE33_19165"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HVT99628.1"
                     /translation="MKKALLFLLAAALSAPAVFAQNDSDAQAAAQKALSSSRYKGIQV
                     SVQNGIATLSGNVDVFASKMSADQKVRHVKGVTAVRDEIQVNGGSEMSDQQLQEKLQK
                     AINYDMVGYGTTAFDAIGVQVHNGVAVLSGHAYGPVDASDAVAIVENTKGVRDVVNNI
                     DVDPVSPMDDQIRLAVYRKVYGFPMLNKYAIDPVKPIRIQVANGHVTLYGTVDTEAEK
                     NAAGIQANSVSGVFSVTNDIVVANATKEKPGK"
     gene            complement(12826..16956)
                     /locus_tag="VHE33_19170"
     CDS             complement(12826..16956)
                     /locus_tag="VHE33_19170"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HVT99629.1"
                     /translation="MPHPTGEPEPLKKEEAPPPKHPHRIRNLVAFGALWTVVVLLVLF
                     GAVAWYASTSSFQNRVRRTVIAELEKSTGGRVELKKFGWRLTHLEFEADDLTIHGLEG
                     PGQVPYAHIDRLFVRLQILSFFRAKIGLNYLEGDHPVLHLIVYPDGSTNQPRPKQAST
                     GNSKDEIFNLAIGRTVIDNGVAILNQRAIPFNLTVDNLAAQVSYVPARDHYVGTLHAE
                     DVLAQRGADLPVHSMIDASVDIGRNTANLVSLTLQSGPQGKSQKTVLRASGNLNNFAS
                     PQWQFSMKGAIDALEVRALTGLTGLAGGTAQIEASGHGSGPQFVIDGNGRISGGAYRT
                     GTVNAGGVTADAVAHVTQDQIAVTGIHARLATGGVLTGEMHILNWNSPAPAANSQPVA
                     QSGQQQGIIRTRLAGFTLDSLLDTVAPPHFRRLGYATTASGTANIDWTGSVENMVGVV
                     NVSLTPPATTPAHEVPVTGEVNAEYRNRAGAVIIHAFEIHTPASEIQVSGSMGVYPLT
                     RPSKIDASLVTRDLAEFNPALGAFGIAVPGEANPIPAELHGQAQFHGTVTGNLRSPEF
                     KGHLQATDFNITALGSNPTANEAASHIHFDSLATDAAYRAGELSVSLGTLVQGKTTID
                     FSGQIRGRTSRPKELFADSSSIHASVQVRNADITQWMALTGKSYPISGTLNLHAQTGG
                     TIGDLDGTGQLTVAGGSVYGEQYRSLTTDVQFRGKDVNANNLVFLMDGGRITGNGGYN
                     FTANTAHADLQGSGFELAHIRRLENHQYPVLGDVDFTVKGSGTLDNPSLQANVHVRNL
                     ELARQFKGLVDAEAHTQGRALQMHVAAHLNSSTLDLTSQTQIAGDYQTQAKLDIAGFN
                     IDPLLQTFSVTGIRGSSSIAGAITVSGPLRRPKQLNGDVRLSQLAVTLEDVPIHSEGE
                     LHASLRDGTFSMDPVHIVGPDTNLTTEGSLGVFDPAKPIHGSAKGSINMSLAQTLDTD
                     IISSGHVDFSMNAEGTVDNPDLTGNVKFTNVNVALEDYVNGLSRMNGELVFDQNRLDF
                     KNVTAYSGGGLIKIGGFVTYHRGLYADLTADAKDVRIRYPEGITSTASAKIRVQGSQA
                     GMIVSGRVMLTGFAVNPDVDFASLAGKGTGVSLPPNPNSPTNKVALDIRVVSAPSLNF
                     QNSFARLAGNVDLAVRGTLAQPSVLGRVTVTEGTATFNGEKFELQHGEIYFSNPVRIQ
                     PVLDLTATTNVENYTITIAMQGSTSKLTPSFRSEPPLSEQDIFSLLAMGRTQEEQQIY
                     STAQSQAGVNTTADSLLGGALNATISNRIQKLFGGGSVRIDPTFVSGIGNATARITVE
                     EPVSKTATLTYATNVNSTAEQLIQGEWRMTPNFSVVAVRDESGVFSLIFRLHRSYH"
     gene            complement(16962..17717)
                     /locus_tag="VHE33_19175"
     CDS             complement(16962..17717)
                     /locus_tag="VHE33_19175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99630.1"
                     /translation="MIALFATLLVASSSRAQKPSPEAPPPPPSSPAASQPIELDHVVA
                     MIGSNVLLQSDVEQEMHFSALEPLRVLPGRNTPDSALRRLIDRTLILEQMKQQQQATS
                     TPAPEVAKSLAGLKKEIPACEEAHCQTAEGWAAFLKSNDLTEQEVEQRWSQRMAMLDF
                     IDERFRSGIRVSQDQIAAYYQKTLVPALEKDNQKAPALADVSSRIREILLQQQVSGLF
                     QDWLGSLRDQGNVQIVDPAYHTDLGSQPSTGEQ"
     gene            complement(17735..21127)
                     /locus_tag="VHE33_19180"
     CDS             complement(17735..21127)
                     /locus_tag="VHE33_19180"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HVT99631.1"
                     /translation="MSGQQFPVRNEGRLGSTAFFGFREHRLWDRTRAAAARPGSWLAG
                     SLVWATLLLAVATLHAQQPVNPTQPATPSSPEDQRKKTPPVEPGSVLPSYNSTRQQTS
                     VKVQAVPPSPSLEGWAGRNIVAIHFEGVPQSTLDPLPSQMEQQPNSPLDPVKVRASLR
                     RLYATGLYKTISVDGKPEDNGIVLTFKGEPTLFLGRVLVRGVKNVHLSNQLNYSTRLN
                     PGTPYTEQKMARAERFLQMTLQQNGYYQAKIVHHTAIDRKNAEIDVQFNVTTGKPARV
                     GAVALQGDSGMTVPAFRKQAKLKAGSKVNLDTVSRALSRVRKHYQKQERLEANVSLAS
                     TQFQPPSNHLDYTFDAARGPVVKIIVEGAKLSKGKIKSLVPIYTEGTLDEDLLNEGSK
                     RIRDYYQREGYFNVKVTNSTDAKGGVTHITYNVNLGPRNRIEAVNIKGNGYFGAWLLK
                     ERLSVQPASFFVRHGIYSQALQEADVDAITALYASNGFIDVKVTPEVHENPHDSSKKE
                     KSLIVTYNIVEGPQKRVGEYKIDGVTPQQMDTIAPRLALQSGQPYSGTNLAQDRDTVV
                     GYFLDNGYDHATVTLHQDPSAKDKDLINVTMNVVPGDQIFIRNVLYSGLKYTHLKTIQ
                     PNILVKPGDPLNQTKLLQTQRDLYNLTLFNQVTTAVENPGGEEPRKNVLIQFDEARRW
                     DVTYGGGFQAQTGTPSTNCPNPLSLIALGINPSNYSCSPNGKFGVSALVELDVSRINL
                     WGRNQSLNFKSQYGSLEKLFTLNYSAPRFFGHPSLDFSFGGGYINAQNVITFASSTSE
                     GDVRMTHHANLKDTLIYQISYRRVKVDANTIQVSPNLIPLLSEPVRVGGPELTWIHDT
                     RRPEPLDAQSGMYNSVQIFATDNSFLSSQANYVHFDWSNATYYGFGREKRYVLARNTR
                     FGMERVFGQDKYEAIPLPERLYAGGPESMRGFPLNSAGPRDSLTGFPIGGAGAFVNQV
                     ELRFPNPQLPWFGRALGFVVFHDMGNIFNNSSDIWPSAIRIRQPHRYTCTDPAYLNAT
                     TQQQVTRSSSTNPTGTCDFNDFSHTVGAGARYHTPIGPIRFDVGYNLNPPVYPVLVQY
                     GGGSSGSSSNASNSNNLTCYTGTQCYGQAAHFNFFFSIGQAF"
     gene            21299..>22237
                     /locus_tag="VHE33_19185"
     CDS             21299..>22237
                     /locus_tag="VHE33_19185"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HVT99632.1"
                     /translation="MAASSPIVAERYSRQVLFAGIGPEGQARIERAHVALVGVGATGA
                     AAAGLLARAGVGTLTLIDRDFVEESNLQRQVLFDEADARDSLPKAEAARRKIAALNGE
                     IRVEAHVVDLVPGNIHELLRAADIVLDATDNFETRYLLNDFAVEQEKPWIYAAAVGSY
                     AATMNVLPRKTACLACIFPEPPGGTVETCDTAGILNSAVNLAASIEVTEALKWIVGAQ
                     DRMRRTLLSCDLWTNEWSEVQAGSPGTGCAVCGGRDFVHLRGEGRPHITLCGRNSVQI
                     HERSRPVDLAEMKERLRPHGKVQSNNLLLRFQRGEHT"
BASE COUNT         4138 a         6088 c         7527 g         4484 t
ORIGIN      
        1 gtacgttcag tgttcttgat atcgaacgcc gcggcgccgg tgccgtaagg gctgttgacg
       61 ttggtgaggc ccgccccgtt gtcatccggg gtgaggacga ccccatcggg accgccgaag
      121 ttgccgaaat tcaggacgtt gtacatggcc actgcgggcg tgatgttcac cttctcaggc
      181 agccagtgga agtggatggg ataccgcaga ctggcgtcga gggtgcgcag cggactattc
      241 gggtaagcgt gggacggcgc agagtccagg gctggcatga cgccgccgag ggcagccagc
      301 tgcgtacccg tgaagatgtt gttggtgatg agggtctggc cggcgggcgt gggcttaccc
      361 gcatagccgg cgttgtattt gtcgatgagt ttgttgaggc tgttgggacc gtactggcgc
      421 atgtaggcgc caggctttgt ggtgggcatc agatcgccaa tttggccatc accgttgtaa
      481 tccgagtgga agatttcgcc ggggccgccg ccctggtcgt cgatcgtgag gccggttggc
      541 ctggcgctgg acaggtgccc gatgagcgag agctgcggtc cgtatttgaa ggtggcggcc
      601 ccgccgaagg acaggatgtg ggtgcgatcg aaccctcccc agcccatgtc ggcagtggta
      661 tcgcgattgt tataggaggg tgaggtgaag aagggatcgg agccggcgga gatgccgttg
      721 tcgccggcgg tcgacagcat ccgggagaag gcataggatg cctggaagtt gctttgccgg
      781 atgccgggca agtggtgcga gatctgctcc atgaagctga cctggagccc gttgtaggaa
      841 gcccggcctt cggggaactg gaaggtgccg acgccgacgt tcggattcac gcccggaaag
      901 gcgggaattc gttttggata ggcggccgcc gggtagccac ctgtgtagga agtgccggaa
      961 tcaaggcccc tggcagcaaa gtcggcgatg gtggcgggac cggccggatt gccattcttt
     1021 gccgggtgca agcccggaca ggcggtgata gcggcgtcaa ccgatccgac ctggcaggcg
     1081 gttagcgtgg ccgctgcagc ggcctgcgcg gatgccaggt tgaagtagcg ggagtcaccg
     1141 acatggttgg cgtcgatggt ctggcccagc ctcagggttt ccagacgaac gtagtcagcc
     1201 gagatgacag ctcctgagaa aagctcgcgc tggacgccta cgctgatatt gatggagtag
     1261 gggaacttga agtccggagc aaaagcgatg gctccgttgt tgatggcaag attgtaggcc
     1321 gccgatccgc tgttgacgcc acctccgctg acttccgcac ggtagtcctt ctgcagttgc
     1381 agccatccgg gggctccgtc cgccagggac atgttgcaga gggcttgaat actgatgccg
     1441 gtgctggtct cagtgagatc gccgagagtg tagttgcctc cgtaacagac gggggtatag
     1501 acggcgaact tgccgttctg caggcgggag gaacgatcga actggacatt gttgaagacg
     1561 ttgctgtcgt aatagattcc gaagccgccc cggagaacgg ttttctgcga gaacgagggg
     1621 ttatacgcga tgccgatgtt ggggccgaag tcccatttgg gttgcgaaac gcggttgccg
     1681 aggccggcgc cccactggtc gaaaaggtgc gaatctccgg agcaggggag gccggagctg
     1741 ccggcgccga aggcgaagtt atccgtggcg atgttggagc acggtacagg cgcgaggtcg
     1801 gagttggagc gtccggtatc gcggtcccaa cgcaggcccc aggtgaaagt gaactgcggg
     1861 ttgatcttcc aggtgtcgac ggcatagagg ccggtgcgcc aatcgctctg gcccccgccc
     1921 ggcagaccga atccctcctt ctcggaggcg taaccgagat tgttggagat gtacacgtag
     1981 taaggcttgt aaccgtgaat gggatcggta gcatcgccga taagggttcc ggaattgatg
     2041 gacgccctgg ggccatagcc gaagaaagcg gcaaggccgc cgccgaggat gcggttcagg
     2101 ctgacgccgt agcggatgtt atggctgcct ctggtccacc cgccgtcgta acggagttgt
     2161 ttgtcggact ggaaggtctg ctggggggca ttctcattgg gcccggtatg caggccttgc
     2221 gccgagtaat agataccgaa gcccgggatc gggttgtaga tgctgttatt gccgagggtg
     2281 gtgtcgccga tctggttgtg gaacttttca aagctgccac ggaagctgtt ggtgaagcgg
     2341 gagctggcaa aatcggcccc gccagcgata ccccaggcgt tgtcgagatt gctgtaggtg
     2401 gaatagacgg cgccggtggc gaaggaattg cgttcgtaat tgccgcggac gaagaagtgc
     2461 gtagcgtggg gtccgttcca gtcgatgcgt ccatcagagt aggtgtcgcg gtcgggactg
     2521 gcgacactcg gaaactccgc ttgtttcgcc ttgaagttaa gaccgagcga gaccggggac
     2581 gactggtcct gcttgaggcg ttcaacgtta gcaaagaaga agagcttgtc gcgaaggatg
     2641 gggcctccga cactgccgcc gaactggttg cgctggaagg ggcttgaagt gtcttcgtag
     2701 gtggcgacgc ccgcgcgctg atcctggaag tattcaaagg cctccccatg gaagctgttc
     2761 gtgccggatt tggtggcgag caggacagag ccctgggagg tgacctccgt ggacggatcc
     2821 gcagtggcgc gcgtgatctg ctcttcgccg atggagccct gcgagatatt gaagatcgtg
     2881 gtgccgaccg tctcgtcagt cacatcctgg ccatcgagaa ggatgcgggt ggtgcggccg
     2941 gaaatgccgc tgaacgaaac cgccgaatat cccgctttgg tcgggtcaaa cccgccatcg
     3001 gaactgccgc cggcctgaag ctgaacaccc ggctggagct gagcaaagtc cagaacgttg
     3061 cggccattga cgggcaggga ttcgaattgc tgcgagctga tcacacccgt gacgctgggt
     3121 tggtccgtat tcaactgcac tgctcccgcg gtcacctcga ctgtctgtgt ctgcgagccg
     3181 accttcagct ggaaggttcc cggcgtggct gtcgcggtac gaatgacggt attcaccacg
     3241 agcgtttcga atccggccgc gctgatcgtg acccggtagt tgcccggatt gagaggaccg
     3301 acagagtaca agccgctggc atcggtcgtg acggtcacga ccgcacccgt gtccgttgcc
     3361 agaatccgca cggaggcgcc ggcaatcacc gcacctgagg tgtcggtgat cgttccctga
     3421 atcgaacctc cattcaccga tatcgcctgc gccgaagctg caggggcgaa acccaacagg
     3481 accgctgccg ctgcaagcgg gatagcggcc aggccgatga tgacgatagt ccgttgccaa
     3541 aagagcagct ttttcataaa agtccccggt ccttatggtt gaaaaccctg agagcgaacg
     3601 ggctgggatg atccgtgtag actcctggcc ccatatcctg caaaaacccc atccatgtcg
     3661 ggagaatgag gtgataacgg gcaattgccg atcccaattt gcaggtgctt gaacgattta
     3721 gctcgtacag ggtggccgga agcggaggag gggcgaacat ctgcctcata agagccaggt
     3781 taatgagcca aactaaagta atcagagggg ttaggatctg gtacaggatt gatctggtct
     3841 tatatccaac cggtaatgct gaatcgcccc ggaatctccc aaaagccata ccagtttcgc
     3901 ggcaaagtat ggcatctcgg aggcgatccc tcgcgatgtt gccaccccaa cgtgcaggag
     3961 ggctggcaca aggcgccagg cgagcgccag gtccagggcc acgctgcttg tgaaaaaatg
     4021 gggttagagc ctctgagggc tctgcaaatg aatgcgctca agactgcttt tttactgacc
     4081 gctttgactc tgctgctggt tttgattggc agtcactggg gtgaaaacgg cgcgattttc
     4141 gcttttgtaa ttgccgccgc catgaatttc ttcagctact tttattcgga caagctggcg
     4201 ctggccatgt atcgggccca gccggtgact cgcgaacagc tgccgcgagc ctatgaggtg
     4261 gtggagcgga tgacgggccg cctcggactg ccgatgccga agatctatgt gattccgacg
     4321 gacgcgccga acgcatttgc gaccgggcgg aatccggcgc atgcatcggt ggcggtgacg
     4381 cacgggattc tgcagctgct gaacgatgac gagctggagg gcgtgctggc gcacgagctg
     4441 ggccacgtgc ggaatcgcga catcctgacc agttcgattg ccgcaaccct ggccggagcg
     4501 attacgttgc tggcgcgcat gggctggtgg gccgaaatgt tcggcggatt cggaggaggc
     4561 ggcgaccggc ggcgcggcgg cggaatcagc gcgctgttca tgctgattct ggcgccgatc
     4621 gccgcgatga tgatccagct atgggtttcg cgcacacggg aatacgaggc ggatgcgacg
     4681 ggcgcgcaca tcacgggcaa tccatatgct ctggccagcg cgctggagaa gctggacgcc
     4741 tattcgaagc gcgtgccgat gccgggaacc gcatcgacag cccatctgtt tattgtccag
     4801 ccgctgctga gccgcgagga ttttgcgggc ttgttctcga cgcatccgcc gattcgcaag
     4861 cggatcgagc ggctgattgg gcggccgagc gtgtacgggc cgcagtgaag ctggtagcag
     4921 gaaccatgct tctgagcgcg gaacgccgtg cgctgaacgc cgtttttatt gcgcccagcc
     4981 gaggccgtcg tcacccttgc cggcattgat gagtttggcg accttccact gcgccaggtc
     5041 gatggcggcg acctggccgg cgcccatgca ggagacatag gcgaatttgc cgtcgggctg
     5101 gatgagcact tcctgcggcg atgccgcgac agggatttca cgagcaacct tcatggactg
     5161 caggtcgatc acggcgactt cattggcgat cggcatagca accagaagcc agcggccatc
     5221 gcgggtagcc gcagagccgt agccgttggc gggcaaaggg acccaggtct tcagcttttt
     5281 cgtcgccgta tcgatgacag cgaggcgggg ctcaaccaca tcagacgtaa aaacgtattt
     5341 atcatcctgg gaaatcgcga tgcgctgcac ttttgtggcg accggaatca cggcgagcgt
     5401 ctttcgcccg gcgagatcaa gcaccgacac tgtgcccggg ccgacgttgg cggtgtaccc
     5461 gaagcggccg tcgtgcgaga gggcgagcat gtgcgactct ttctggccgg tgggaacttt
     5521 accaacaatc ttcagcgtgt gcggatcgat caccgtgatg gcgttgtcga gttcggtggt
     5581 gacgtagagg cggttgtgcg ctgcgtcgag tacgggaaga tgcgggcgca ccgggtggcc
     5641 gaagtcgacg gtgcctgtga tttttcgcga ggctacgtca atgaccagca tcttgctgcc
     5701 atcgattccg ggcttgccga cgccggagtt gccgtagatg ggcaggaagg ctgtgtgccc
     5761 gtcggcggtg gccgtgatct cgtgacccca ctggtccgag acggtttcgg cgatggtcgc
     5821 gacttccctg ccctgagcgg catcgatgac gctgacggtg cggtcgcgct gattggcgac
     5881 cagcaggctc tgggctgaag cggcggcaga gaaacatgta acggcgacaa aaacaaatac
     5941 cgggatgcgc tgagtcatag aggttgccag tctaacgcgg cgcgggccgg gttgcgaatc
     6001 gggatcacag ctgagaccgt ccggcagccg ccgggcagcg aatccctgta ctcttgttgg
     6061 catgaaaagc gtgctgcgca ccctcgtgtg gctggcgatt gtcgtctggt tcggcgggtt
     6121 gctgttcttt cccatcacgg cgtgggctgc gttcagcacc atcgccgaca cgcaccaggc
     6181 gggcacgatc gtggcaaagt gcctgggcgt cctgcatcac gaaggcctgt ttgcgggctg
     6241 ccttatcgtg ttcctgcttg cgctcggcta tttcctgaag gccttcagga tgagcaccac
     6301 atccatcggc attgcagtaa cgctgctgat gatcggctgc accgtgtggt cgcagtacag
     6361 cattattccc cgaatggagc gggaccgcat cgccgcaggc ggagcgatcg acagcgtgcc
     6421 gaagaccgat ccgcggcatg tggattttga ccggctgcac aacgtgtcga ccgatgtgga
     6481 agaggccgtg atgctgggcg gactggtgct ggtggttttg ctggcgaggg attactaagc
     6541 acagagcccg gctcagacct cagtttggag ttacattccg ccggctttgg cggcaagagc
     6601 ggcgacgacg ctgatgttct tcaccaggtc ggacgggagg ttgtgccgct gctgcaggta
     6661 ttcagggctg ttccaggaga gccagacagc gccgtcggtg tcttcccaga cgagaatctt
     6721 caaaggcaga tcgatggcga tcgtgggtgc ggcgagcatg agcggcgtgc ccgcggcggg
     6781 atttccgaag atgagaagct tcgtttcccg catttccagc cccgcctttt ttgcttcgcc
     6841 gctgtgatcg accagagcga acagcttcac ccctttggca tcgaggatgc ttcggaggcg
     6901 ggccaccgtc tcctcaacgg agtgctggct acgctggctc aggaatccct gggcgtcggc
     6961 cgtcatcgat tgttctccat tcatctctcc cgtcgaacat tgctcgtggc gctgtttgtt
     7021 ctcagttatc tttgcagacg ctctcggctt ccgtgaaatg aaaacgggaa tccgccagtc
     7081 cggattgccg tttgttttgc gcgtacgaaa tctggatcga tctcaggatg ccttgcgctc
     7141 tttcatcggc tcgccgttga tgctctgctc gcgcccgtct ccctcaatgt cagaatcagc
     7201 gcctccaccg ttgctgccca tggtcagcgg catcgaggct gcggcccaac gcggtagggg
     7261 gggccctcac gggttccgga gtattcgggc cggcgtcctg cgaagaccca ggcggagcca
     7321 gaacggcacc gggtggaagg acacgcgtcg catgcgtgat cttatgcggt cttctccggc
     7381 gtactcgctc ttgggatttc agacatcaga gccagggcgt tcccggcggg gtcgttgaaa
     7441 atggcgagcc agaggtcatg gctcttcatg cgcgcaacca gatgaggctc ctgtgagaag
     7501 ccaacccctt tttccctcag cgaggcgcag gcggtctcaa tatcaggaac gcggaagtag
     7561 agaacggttc cgccaccagg cgccgcgtga ttctcgccgg tgccgagcag cagacgtaca
     7621 gagccgcact gaaagaaggt catggcgccg gcgtcgaaga ggaactgcat gccaaggatg
     7681 tcgcggtaga aggcgcgggc gtcgtcgagg ttcgtgactg tgagcgcgat ctgaccgatg
     7741 tcgtggaggt ggacagctgg attcgtggcg gtctgcatgg aatcttcctg ttcgttagct
     7801 gagctaatga tttagcatga agccggatac ggatgtcaac tgcaagcgat cctgcccggg
     7861 aagagctggc cgactccctg cactcggccg cgatccacct gctgcgcaaa ctgcggcgtg
     7921 cggatgtgcg ttcccggttg aatgccccac gtctgtcggc gttgtcggta atcgtgtttg
     7981 ggggtccgct gacgctgggg cagctcgcgg cggccgagca ggtgaggccg cccaccatga
     8041 caaggattgt gcacgcgctc gaggccaggg gtctggtgaa aaagaactcg aatgccgagg
     8101 accggcggac ccttcacttg tctgcaacca caaaaggccg gcgagtgatg cacgaggcgc
     8161 gggccagacg gatacactca ctggcaacgc aaatcgagaa gctgtcccgg cgggagcaag
     8221 agaggttgcg ggatgggctg gagatcatcg gttccctgat ccgcgaactt taagcgaaag
     8281 ttacgctccg tcacattcat ggaaccgggg cacgaacatc tccgtatggg actccaggcc
     8341 atcgagcgag gtgcccaggg tgaagctctg cattgagaat gtcggtaagc ggtacggcag
     8401 caacgtgtgg gcgctgcggc gtttcagcct ggatctgaag cccggcgtgc tgggactgtt
     8461 ggggccgaat ggcgccggca aaacgacgtt gatgaacatc ctggcgacca tcacccgcgc
     8521 cagtgagggc acgatcacgt ggaacggcgc cgaggtggcg aaggatccga atgcgctgcg
     8581 cgccgtgctg ggctatttgc cgcaggattt cggcgtgtat ccaaacctga gcgcgcagga
     8641 attcctcgaa tatttggccg cggtcaaggg gctggatgca accgcggcgc ggcggcgcat
     8701 cgatcagctg ctgaacgtgg tgaatctggc cgatgtgcgg ggacgaccgc tgggtggatt
     8761 ctccggcgga atgaagcagc gagttgggat cgcgcaggcg ttgctgaatg atcctcggct
     8821 gctgattgtg gatgagccga cggcggggct ggacccggag gagcgggtgc gctttcgcaa
     8881 cttgctgtcg gagctttccg gggagaggat tgtaattctt tcgacgcaca ttgtgtccga
     8941 cgtcgaagca accgcgaccg agattgcgct gatctcgaac gggtcgctgg tggcgcatgc
     9001 ctcgccggag gaactgctgc ggcgcgtcga cggccgggtg tgggagtggg tgattccaag
     9061 cgcggatctg aacgcggtga agcaggccta caaagtaagc aacacggcgc ggcgcagcga
     9121 tggcgtgcat gtgcgcatcc tgacggagga accgccggac ggggcggcgc cggtgccgcc
     9181 gacgcttgag gatgcgtatc tcgattcgct ttcggtctat cgcgcaggag tggcggcgtg
     9241 agcagcgcgc gggtgctttg gcatctggtg aaggcggatt tcctgacccg cgtgcggcgc
     9301 acgagctttc tcgtcacgct cggcttcgcc gtgttcgccg gatatcaggt gttcgcgggc
     9361 aacgtgcaga tgcagctgga cgattatcgc ggcgtgtaca actcggcgtg ggtgggcgcg
     9421 ctgatgggcg tggtgacgag cacgctgctg acgctggtgg gcttctacat tgtgaagggc
     9481 tcgattctgc gcgatgaaac gacgcacgtg gggcagatcc tggccgcgac accgatgagc
     9541 agaggcttct acacggtggc gaagtggttg ggaaattttt ctgtgctggc ggctatagtc
     9601 gcggtgatgg gcctggctgc ggtagccatg cagctgatgc gaggtgagga tcgcacgctg
     9661 cacctgtgga cgctgctttc gccgctcatt ctgtacgcgc tgccggccat ggcctttgtg
     9721 gcagcgatgg ccgtgctgtt tgaaaccctg cctctgctgc gcggcggggt gggcaacgtg
     9781 gcgtggttct tcctgtggat tggaatcctg gtggtcgggg tggggacatc ggcggagcat
     9841 ggcggtgcgg tgctgcgggg agaatatttt cgggacttca gcgggatgcc aacgctgatg
     9901 gggcagatgc gaaccacgct ggtgcaggta gatccgcact tccgcaacaa tttttccctg
     9961 gcgatcggcg gcatgaagcc gcacaaacaa ttcttgtggg agggcgtgga gtgggacgcg
    10021 gcccaggtaa cagcgcggct gctgtggttc gtgtatgcgc tcgccaccgc gctgctggcg
    10081 gccattttct ttcatcggtt cgatcccggc cgggaaggat ttgcgcgaaa acggaggacg
    10141 atgcaggagc gggcgcccgt ggtggagctc gcggcggcga aaaacctggc acaggcggat
    10201 ccatggacgc atcggctgtc gcccctggaa ccgacggccc cgaagaaccg gttctttgcc
    10261 ctcgtcgcgg cggaactgcg cgtgatgctg cagggccatc ggtggtggtg gtacgcgatt
    10321 gcagccgggc tgttcgtggc ctgtgcggcg gcacccgtga aggcttcgag cggaggcgta
    10381 attctggcgg cgtggatctg gccgctgctg atctggtcca agatgggcgg acgggaatcg
    10441 cgatacgcga ccggcgcgct gattttttcg gctccgcatg cgctggaccg gcaactgccg
    10501 gctgcgtggg tggcgggtgt gctgatcacg ctggcgaccg ggggcgggtt gctgatccgc
    10561 ctgctcatcg ccgcagacgg acgcgcggtg gcgggtttgc tcgttgccgt gtgctttatt
    10621 ccgagcctgg ccctggcgtg cggaatcctg accggtggca gcaagttctt tgaagccctg
    10681 tacacggtgg gttggtatgc aggcgccgcg catcacattc gcgggctgga ctttatgggg
    10741 acggtggatg cgagcagcac gcctgcgatt tatctggcac tgacagtggc gctgctggcg
    10801 gtgtgctgga cgcgaagacg gatgcagctt gcctacgcgt gacgccggat cagaaggtcc
    10861 gcagcgacaa ataagaaaaa gatgacggcg atcacgccgt tcatggtgaa gaaggcggcg
    10921 ttcaggcggg tcaggtcgcg cggggagacc agcgtatgct cccacagcag cagcgcggcg
    10981 acggcgacaa tgccggctgc ggcgatgggg ccgaggtgga acagtacggc cagttcgatc
    11041 agcagggcga gcatgagcag atgcataccg cgcgcaatcc aaaatgctga tgcgaggccg
    11101 agcgtggcag gaatgctgta gagtccggcg gtgcggtcgt gctgctcatc ctggcatgcg
    11161 tacaggacgt cgaagccggc aacccagaag agcacagcga gcgtgagcac gaggatgcgg
    11221 gcatcgagag caccgcggat ggcgatccac gcgccggtcg gggcaatgcc gagagcgagt
    11281 ccgaggaaaa ggtgcgacca gcgcgtgaaa cgcttcgtgt aggagtaaag gaacaggatg
    11341 accagaacca cgggcgaaag gatcagcgtg agctgattga ggcgccaggc tccgaagagg
    11401 aagagggctg acatcaccag cgtgaacccg gcggcgaagt tgcgggcaag aagtccggcg
    11461 gggatcgcac gcaggcgggt gcgggggttc gcagcgtcga ggtccgcatc cgcccagcga
    11521 ttgaaggcca tagcggcgga gcgcgcggag atcatgcaga gcacgatcca ccccagcttc
    11581 gccgcgagcc atccagcgtg cgcggcgggc ggaaatccgt gcgctgccag ggcggccccg
    11641 gtgagcgcga aggggagagc gaagatcgag tgctcccact tgatcatctc gagcgtgatg
    11701 cggatgcggt ggagcatatt tccagcgtat agcggatagc gttcagcgga tagggaccgg
    11761 tcccgatcag gacagctttc gcggttggca aaagaaagcg gcaccctgtt gagggtgccg
    11821 cgcaaggtga gtctgcaggt tgatggcgat ggctattacg cctatcgcta ctttccaggt
    11881 ttttccttcg tggcgttcgc gaccacgatg tcattcgtta cgctgaaaac accggaaacc
    11941 gagttagcct ggatcccggc agcgttcttc tccgcttcgg tgtcgacagt gccgtagagc
    12001 gtgacgtgtc cgttggccac ctggatgcgg attggcttca ccggatcgat ggcgtactta
    12061 ttcaacatgg ggaaaccata gaccttgcga taaaccgcca ggcggatctg gtcgtccatc
    12121 ggcgagacgg gatcgacatc gatgttattc acgacatcgc gcacgccttt ggtgttctcg
    12181 acgatggcaa cggcgtccga ggcatcgacc gggccataag cgtggccact caggacggca
    12241 acgccgttgt ggacctgaac gccaatggca tcgaaagccg tggtgccgta gccgaccata
    12301 tcgtagttga tggctttctg cagtttttcc tgcagttgct gatcggacat ttcactgccg
    12361 ccgttcacct gaatctcatc gcgaacagcg gtgacaccct tcacgtgacg aactttctgg
    12421 tcggcgctca tcttgctggc gaagacatcg acgttcccgc tgagggtcgc gatgccattc
    12481 tgcaccgaca cctgaatccc tttgtaacgc gaggaactca gcgccttttg tgctgcggcc
    12541 tgagcgtcgg agtcattttg cgcaaacacc gcgggagcag aaagagccgc ggcaagaagg
    12601 aaaagcaatg ctttcttcat atttattctc ccgggccgtc tccaaaattt ttcggggaat
    12661 ccgaccggcg taccgcctgc cggggagaca ggcctttcgt tctattcaga cacggaaagg
    12721 catcatttgg atgcgccgct ggggataaaa atcggtttag gctcgatgtt gcttcgcgat
    12781 cgggaaccaa tgaatgcggt tgcaatcagc aaccccgcgc cacagctagt ggtaactcct
    12841 gtggagcctg aagataaggc tgaatacgcc cgattcgtcg cgcaccgcca cgactgagaa
    12901 attgggcgtc atgcgccatt cgccctggat caactgctcc gcggtggagt taacgttggt
    12961 ggcgtaagtc agggtcgcgg tcttagagac gggctcttcc accgtgatgc gggcggtggc
    13021 attgccgata ccgctgacga aggtaggatc gatgcgcacg ctgccgccgc cgaaaagttt
    13081 ttgaatccga ttgctgatgg ttgcattcag agcgccgccg agcagcgaat cggcggtggt
    13141 attgaccccg gcctgggact gcgccgtgga gtagatctgc tgctcctcct gggtgcggcc
    13201 catcgccaga agagagaaga tgtcctgctc ggaaagcggc ggctcggagc gaaaactcgg
    13261 cgtgagcttg ctcgtggagc cctgcatggc gatggtgatg gtgtagttct ccacgttggt
    13321 ggtggccgta aggtcgagca cgggctggat acggacggga ttgctgaagt agatttcgcc
    13381 gtgctgcaac tcaaatttct cgccgttgaa tgttgccgtg ccctcggtga ccgtaacgcg
    13441 gcccagaacc gagggctggg ccagtgtgcc gcgaaccgcc aggtcgacgt tgcccgccag
    13501 gcgggcgaag gaattctgga aattgagaga gggcgcggaa acgacgcgga tatcgagtgc
    13561 gactttgttc gtgggcgaat tgggattggg tggaagcgat acaccagtcc ctttgccggc
    13621 cagtgacgcg aagtcaacat cggggttgac ggcaaatccg gtgagcatga ctctgccgct
    13681 gacgatcatg ccggcctgac ttccctgcac ccggatcttc gcgctggcag tcgacgtgat
    13741 tccctccgga tagcgaatgc gaacatcttt ggcgtcggcc gtgaggtcgg cgtacagacc
    13801 acggtggtag gtaacgaagc caccaatttt gatgagaccg ccaccgctgt aagcagtaac
    13861 gtttttgaag tcgaggcgat tctggtcgaa gacgagctcg ccgttcatgc ggctgaggcc
    13921 gttgacatag tcctcgagcg cgacgttgac attggtgaac tttacgttgc ctgtgagatc
    13981 cggattatca acggtgcctt cggcgttcat gctgaagtcc acgtggccgg aggagatgat
    14041 gtccgtgtcc agggtctgcg ccagagacat attgatggat cctttggcgc tgccatggat
    14101 gggttttgcg ggatcgaaga cgccgagcga gccttcggtc gtgagattgg tgtccgggcc
    14161 gacaatgtga accgggtcca tgctgaaggt gccgtcgcgg agcgaagcgt gcaactcgcc
    14221 ctcgctgtga atgggcacgt cctccagcgt gacagcgagc tggctcaggc ggacatcgcc
    14281 gttgagttgt ttcggacgcc gcagcggccc ggagaccgtg atcgctccgg caatggacga
    14341 gctgccgcga attccggtga cgctgaaggt ttgcagcagt ggatcgatat tgaagccggc
    14401 aatgtcgagc tttgcctgag tttgataatc accggcgatc tgcgtctggc tggtcagatc
    14461 taaagtggac gaattgagat gtgcagccac atgcatctgg agtgcacggc cctgggtatg
    14521 ggcttcggcg tcgaccaatc ctttgaactg gcgggccagc tcaaggttgc gcacatgcac
    14581 gttggcctgg agcgaaggat tgtcgagcgt accgctgcct tttaccgtga agtccacatc
    14641 gccgagcaca gggtactggt ggttttcgag gcgacgaatg tgagccagct caaagccgct
    14701 gccctgaaga tcggcgtggg ctgtatttgc ggtgaagttg tagccgccgt ttcccgtgat
    14761 cctgccacca tccattaaaa agaccaggtt attcgcgttg acgtcttttc cgcggaattg
    14821 cacatcggtg gtcagagacc gatactgctc gccgtaaacc gatccgccgg ccacagtcaa
    14881 ttgtcccgtc ccgtcgaggt ccccgatcgt gccgccggtt tgagcatgga gattcagcgt
    14941 ccccgagatc ggatagcttt tcccggtgag ggccatccat tgggtaatgt ccgcgttcct
    15001 gacctggacg ctggcgtgga tggaggacga gtccgcgaaa agctccttcg gccggcttgt
    15061 gcggccgcga atctggccgg agaagtcgat ggttgtcttg ccctgaacca gggtgccgag
    15121 actgaccgag agttcgccgg cgcggtaggc tgcatcggtg gcgaggctgt cgaaatggat
    15181 gtggctggcg gcctcgttgg ccgtgggatt ggaacccaga gcggtgatgt tgaagtccgt
    15241 ggcctgcaga tgccctttaa actccggcga acggaggttt ccggtgaccg tgccgtggaa
    15301 ctgcgcctgg ccgtggagct cggctggaat cggattggcc tctccgggca cagcgatgcc
    15361 gaaagcgccc agcgccggat tgaattcggc gaggtcgcgg gtcaccaggc tggcgtcgat
    15421 tttcgacggg cgcgtcagcg ggtagacgcc catgctgccg gaaacctgaa tctcggaagc
    15481 cggcgtatgg atctcgaagg cgtggatgat cacggctccg gcgcggttcc tgtattcggc
    15541 gttgacctcg cccgtgaccg gaacttcatg ggccggagtg gtggcaggcg gggtgagact
    15601 cacgttaact acgccgacca tgttttcgac gctgccggtc cagtcgatgt tggcagtacc
    15661 ggaggcagtg gtggcgtagc cgagacgacg gaagtgcggc ggcgcgacgg tatcgagaag
    15721 cgagtcgagg gtaaagccag ccagccgcgt gcggatgatt ccctgttgct gcccggactg
    15781 cgcaacgggt tgggagttgg ccgccggagc cggtgagttc cagttgagga tgtgcatctc
    15841 gccggtgaga acgccgccgg tggccaaacg cgcgtggatg cccgtgaccg cgatctgatc
    15901 ctgggtgaca tgagcgactg catcggcggt gacgccgccg gcattcacgg tcccggtgcg
    15961 atacgcgccg ccactgatgc gtccattgcc gtcgatgaca aattgcgggc cggaaccgtg
    16021 gccactggct tcgatctggg cggtgccgcc tgccagtccg gtcaggccgg tgagagcacg
    16081 aacttcaagc gcatcgatgg cgcccttcat gctgaattgc cactgcgggc tggcgaagtt
    16141 gttgaggttc ccggaggcgc ggaggacggt cttctggctc ttgccttgcg ggccgctttg
    16201 cagggtgagg gagacgaggt tcgcggtgtt gcggccgatg tcgaccgagg cgtcgatcat
    16261 tgagtgaaca ggaaggtcgg cccctcgttg cgcgaggacg tcttcagcgt gcagcgtgcc
    16321 gacgtagtgg tcccgcgccg gaacgtagct gacctgcgcg gcaagattgt cgaccgtgag
    16381 gttgaacggt atggcgcgct ggttgagaat cgcaacgcca ttgtcaatga cggttcggcc
    16441 aatggcgaga ttgaagatct cgtctttgct gttgccggtg gaggcctgct tcggccgggg
    16501 ctgattcgtc gagccgtcgg gataaacgat cagatgaaga accgggtggt ccccctcaag
    16561 gtagttgagg ccgatctttg ctcggaagaa ggagagaatc tgcaggcgga cgaaaaggcg
    16621 atcgatgtgc gcgtaaggga cctggccggg gccttcgagg ccatgaatgg taaggtcgtc
    16681 ggcttcgaat tccaggtggg taagcctcca gccgaacttt ttgagttcga cgcgaccgcc
    16741 ggtggatttc tccagttcgg cgataacggt gcgccgaacc cggttctgaa aggaggaggt
    16801 actggcatac caggcgaccg ccccaaaaag aaccagcagc acaacgaccg tccacagcgc
    16861 accgaacgcc accaggttgc ggatgcgatg agggtgcttc ggcggcggtg cctcttcttt
    16921 tttcaggggc tcgggttcgc cggtgggatg tggcatcgcg ttcattgttc ccccgtcgac
    16981 ggctgcgatc ccagatcggt gtggtaggcc gggtcgacga tctgcacgtt gccctgatcg
    17041 cgcagactcc caagccagtc ctgaaacagg ccgctgacct gctgctggag gaggatctcc
    17101 cggatgcggg aggagacatc ggccagggcc ggagctttct ggttgtcttt ctccagggcg
    17161 ggcaccaggg tcttctgata gtacgccgca atctggtcct gcgagacgcg aatcccgctg
    17221 cggaagcgtt catcgatgaa atcgagcatc gccatgcgct ggctccagcg ctgctccact
    17281 tcctgttcgg tcaggtcgtt ggacttcaga aacgcagccc acccttctgc agtctggcag
    17341 tgggcttcct cacaggctgg gatctccttc tttaacccgg cgagggattt cgcaacctcg
    17401 ggcgcgggtg tggaggtcgc ctgctgttgc tgtttcatct gctccagaat cagggtccgg
    17461 tcaatgagac gccgcagcgc tgaatccgga gtattccggc cgggcaacac gcgcagcggc
    17521 tccagcgccg agaaatgcat ttcctgttcg acgtcgcttt gcagcagaac attggaacca
    17581 atcatggcga ccacgtggtc cagttcgatg ggctggctgg cggcaggcga cgacggcgga
    17641 ggcggaggag cctcgggaga tggcttttgg gcacgcgatg acgacgcgac gagcagcgtt
    17701 gcgaagagag cgatcatagg ggcccggtat ctcatcagaa tgcctgcccg atactgaaga
    17761 agaagttgaa atgtgccgcc tgcccatagc actgtgtgcc tgtatagcag gtcaggttat
    17821 tcgaattgct ggcgttgctg ctgcttccgc tgctgccgcc gccgtactgt accaggaccg
    17881 gatagacagg cggattcagg ttatagccta cgtcgaagcg aatcggcccg atcggcgtgt
    17941 gatagcgagc gccggctcca accgtgtggc tgaaatcgtt gaagtcgcag gttccggttg
    18001 ggttcgtgct cgaggagcgc gtgacctgct gctgggtggt cgcgttcagg taggcagggt
    18061 cggtacaggt atatctgtgc ggctgccgga tgcgaatcgc gctgggccag atatcggaag
    18121 aattgttgaa gatgttgccc atgtcgtgaa agacaacgaa gccgagcgct cgcccgaacc
    18181 atggcagctg cgggttggga aagcggagct ctacctggtt gacgaacgcg ccagccccgc
    18241 cgatcgggaa gcctgtcagc gaatcccggg gcccggcgga attgagcggg aagccgcgca
    18301 tcgattcagg accgccggca tacaggcgct ccggcaaagg gatggcttcg tacttgtcct
    18361 gcccgaagac gcgctccatg ccaaagcgcg tgttgcgcgc gaggacgtac cgcttttccc
    18421 tgccgaaccc gtaataggtc gcgttggacc agtcgaaatg tacgtaattc gcctgggaag
    18481 aaagaaagga attgtcagtc gcgaagatct gcacgctgtt gtacataccg ctctgtgcat
    18541 cgagaggctc cggacgccgc gtgtcgtgga tccaggtcag ttccggcccg cccacgcgaa
    18601 ccggttctga gagcaggggg atgaggttcg gtgaaacctg gatcgtattg gcatcgacct
    18661 tcacccggcg gtaggagatt tgataaatca gggtgtcctt gagattggcg tgatgcgtca
    18721 tccgcacgtc gccttccgac gtggaggagg caaatgtaat gacgttttgc gcattgatgt
    18781 agccaccgcc gaaagagaaa tcaaggctgg gatggccgaa gaagcgcggc gcggagtaat
    18841 tcagcgtgaa cagcttttca agggaaccgt actgcgactt gaaattgagc gactggttgc
    18901 ggccccacaa attaatgcgc gagacatcga gttcaaccag agcgctgacg ccgaacttcc
    18961 cgttgggact gcaggagtag ttcgatggat tgatgccgag cgcgatcagg ctgaggggat
    19021 tggggcagtt cgtgctgggc gtgccggtct gcgcctggaa gccgccgcca taggtcacat
    19081 cccagcgccg ggcctcgtcg aactgaatga gcacgttctt gcgcggttct tcaccgcccg
    19141 gattttccac cgcggtggtg acctgattga aaagggtcag gttgtaaaga tcccgctgcg
    19201 tctgcagaag tttcgtctgg ttgagagggt caccgggctt gaccagaata ttgggctgga
    19261 tggttttcag gtgggtgtat ttgaggccgc tgtaaaggac gttgcggatg aaaatctgat
    19321 ccccaggtac cacgttcatg gtcacgttga tcagatcctt gtccttcgct gacggatcct
    19381 ggtgaagggt gacggtcgcg tgatcgtagc cgttgtcgag gaaatagccc acaacggtat
    19441 cgcgatcctg ggccagattc gtccccgagt acggctggcc tgactgcaga gcgagccggg
    19501 gcgcgatggt gtccatttgc tgcggagtca cgccgtcgat tttgtactct ccaactctct
    19561 tttgcggacc ctcgacaatg ttgtacgtaa cgatgaggct cttctctttc ttgctcgaat
    19621 catggggatt ctcgtgcact tcgggggtca ctttcacatc gataaagccg ttgcttgcat
    19681 agagcgctgt gatcgcgtcg acatcggctt cctggagggc ctgactgtaa atgccatgcc
    19741 ggacgaagaa cgaagccggc tgaacgctca gccgctcttt gagcagccat gctccgaaat
    19801 agccgttgcc cttgatgttc actgcctcaa tgcgattccg tggcccgagg ttaacgttgt
    19861 aagtgatgtg ggtgactcca cccttcgcat cggtggaatt cgtgactttg acgttgaaat
    19921 agccctcgcg ctggtaatag tcgcggatgc gcttgctgcc ctcgttcagc aggtcctcgt
    19981 ccagggttcc ctcggtataa atcggaacca ggctcttgat cttgcctttg ctgagtttcg
    20041 ctccctcgac aatgattttg acgaccggtc cgcgggccgc atcgaacgtg taatccagat
    20101 ggttggaagg cggctgaaac tgcgttgagg ccagactcac gttggcctcc agccgctcct
    20161 gcttctggta gtgctttctg acccggctga gcgcgcggct gacggtgtcg aggttgacct
    20221 tcgatccagc cttgagtttt gcctgctttc ggaaggcggg tacggtcatg ccgctgtccc
    20281 cctgcagggc gaccgcgccc acgcgagccg gttttcccgt tgtcacgttg aactgcacgt
    20341 cgatctcggc attcttcctg tcgattgccg tgtggtgcac gattttggcc tgatagtagc
    20401 cgttttgctg cagggtcatc tgcaggaaac gctcggcccg agccatcttc tgctccgtgt
    20461 acggagtacc agggttgagc cgggtggagt agttgagctg gttgctgaga tggacgttct
    20521 tcaccccgcg caccagcacg cggccaagga agagcgtggg ctcaccctta aaggtcagga
    20581 cgatcccgtt atcctcgggc tttccgtcga cggagatggt cttgtacaga ccggtggcat
    20641 acaggcggcg caaactggca cgcaccttta ccggatcgag agggctgttg ggctgctgct
    20701 ccatctggct gggcagtggg tcaagcgtcg actggggaac gccttcgaaa tgaatagcga
    20761 cgatgttgcg gcccgcccaa ccctcaaggc tcggagaggg gggtaccgcc tggactttga
    20821 cggaggtctg ctgccgggtg gagttgtacg acggcagaac actgccgggt tcgaccgggg
    20881 gtgtcttctt tctctgatct tcgggagaac tgggcgtggc aggctgggtg gggttgacag
    20941 gctgctgagc atgcagcgtc gctacggcca gcaacaacgt tgcccagacg agcgaccctg
    21001 cgagccagga gccagggcga gccgcggccg ccctcgtgcg gtcccatagc ctgtgttcac
    21061 gaaaaccaaa aaatgccgtc gaacccaaac gcccctcgtt ccgcaccgga aactgttgtc
    21121 ccgacaaccc gagaacttct gtgctgttga acaccgctgg ctggcccagg tttctccgga
    21181 aacgccgttc aacgcctccg agtttcgctc tcgaagtgtg agatgccaac tcggcgtcag
    21241 cgtggccgga tcagaatgac agcagccata gcaatgaagt ttctatacta ctgaccagat
    21301 ggctgcctcg tctccaatcg ttgccgagcg ttactcgcgc caggtgctgt tcgccgggat
    21361 cggtccggag ggtcaggctc gaatcgagcg cgcccatgtg gctcttgtcg gggtaggggc
    21421 gaccggggcg gccgccgccg gcctgctggc gcgcgctggc gttggcacgc tgaccctgat
    21481 cgaccgcgat tttgtcgagg agagcaatct ccagcgccag gtcttgttcg acgaagccga
    21541 cgcgcgggat tcgctgccga aagcagaggc ggcccgccgc aaaatcgcgg cactcaacgg
    21601 cgagatccgc gtcgaggccc atgtcgtgga tctggtaccg gggaatatcc acgagctact
    21661 gcgtgccgcg gacattgttc tggacgcgac tgacaacttc gagacccgct acctgctgaa
    21721 cgactttgcc gtggaacagg aaaaaccatg gatctacgcc gccgcggtgg gctcgtatgc
    21781 ggcgaccatg aacgtcctgc cccgtaaaac agcctgcctg gcctgcatct tccctgagcc
    21841 gcccggtggc acggtggaga cgtgtgatac agcgggaatc ctgaacagtg cggtgaacct
    21901 ggccgcctcc atcgaggtga ccgaagcgct gaagtggatc gtcggggcac aagacaggat
    21961 gcggcggacc ctgctctcct gcgacctgtg gacgaatgaa tggagtgaag tgcaggccgg
    22021 tagtcccgga acaggttgcg ccgtctgcgg cggccgcgac ttcgtccacc tgcgaggcga
    22081 gggtcggccg cacatcaccc tgtgcgggcg gaactcggtg cagatccacg agcgctcgcg
    22141 cccggtcgat ctcgcggaga tgaaagaacg gctccgcccg cacggcaagg tacagagcaa
    22201 taatttgctg ctccgcttcc agcgggggga gcacacg
//
LOCUS       DASZYN010000749         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_110126, whole genome shotgun sequence.
ACCESSION   DASZYN010000749 DASZYN010000000
VERSION     DASZYN010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110126"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..1595
                     /locus_tag="VHE33_19190"
     CDS             342..1595
                     /locus_tag="VHE33_19190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HVT99633.1"
                     /translation="MQLQTILNRVARHSSFVYGTPHWSDDPLRPMLEVPIRPRSNSRP
                     ICSGCGKRRPGYDRLKERRFDFVPLWQIAVVFVYAMRRVDCPTCGVVVEQVPWSTGKS
                     QLTTAYQWFLAGWARRLSWQEVAAVFHTSWEHVRDSVRYAVTWGLVHRDLSGVTAIGI
                     DEIQWRRGHHYLTLIYQIDEGCRRLLWIGRERTEASLRQGLDLLGESFCSGLRFVCSD
                     MWQPYLKVLAEQAGAAIHVLDRFHVMKQLGEAIDEVRAAEAKRLQQDGYEPVLKRSRW
                     CLLKRPENLTEQQTVKLSELLRYNLQSVRAYLHREDFQRFWEYQSPFWAGKFLDEWCT
                     RVMRSRLPPLKKVARSLRKHRPLLLNWFRARGAISAGIVEGFNNKAKLTMRKSYGFRE
                     YETIQLALYHQLGDLPEPEFTHKFC"
BASE COUNT          340 a          538 c          557 g          366 t
ORIGIN      
        1 cggcagaagg cccgcgtccg aggtcaactg tccggctgtg ggctcaacaa cgatgggctt
       61 ggcatcgaaa aaatcgggtc ttcagcggaa tctgtgggtg aagtcccgtt agtttcgccc
      121 tcgttttagg tttgggggag aagggggcct gtggctcggg cgaccggggg ctcattgccg
      181 ccccctggac cccgctagcc tttctctgtg tccgttttcg aggacgccgg agagcgggac
      241 gccgccgagc ggcgaaaacg ccgaggcaaa caagtgcatc ctgagcagcc gtgggccacg
      301 atggagttac cacgcttcgt cgtcgcccag gccaactcag gatgcaactc caaactatcc
      361 tcaatcgtgt cgcgcgtcac tcttcgttcg tctacggcac accgcactgg agcgacgatc
      421 cgctgcgacc gatgctggaa gtgccgattc ggccacgttc caacagccgc cccatttgct
      481 ccggctgcgg caagcgccgg cctggctacg atcgtctcaa ggagcggcgg ttcgacttcg
      541 tgcccttgtg gcagatcgcg gtcgtgttcg tctatgccat gcgacgcgtc gattgcccga
      601 cctgcggcgt ggtcgttgaa caggtgccgt ggtccacggg caagtcgcaa ttgacgacag
      661 cttaccaatg gttcttggcg ggttgggcac ggcggctttc gtggcaggaa gtcgccgcgg
      721 tgttccatac cagttgggaa cacgttcgcg actcggtgcg gtacgcggtg acttggggcc
      781 tggttcatcg agatctgagc ggcgtgaccg ccatcggcat cgacgagatc caatggcgcc
      841 gcggtcatca ctacctgacg ctgatctacc agatcgacga gggttgccgc cgactgctct
      901 ggatcggccg cgagcggacc gaagccagtt tgcgtcaagg attggacctg ttgggtgagt
      961 cgttttgttc cggcctgcgg tttgtgtgca gcgacatgtg gcagccgtac ctcaaggtgc
     1021 tggccgagca agcgggagcc gcgatccacg tgctggatcg ctttcacgtg atgaagcaac
     1081 tgggcgaagc gattgacgag gtgcgcgctg ctgaggcgaa acgtttgcag caagacggtt
     1141 acgagccggt gctcaaacgt tcgcgttggt gtctgctgaa acggccggag aacctgacgg
     1201 agcaacagac cgtcaagtta agcgagttgc tgcgttacaa cctgcaaagc gtgcgagcct
     1261 atctgcaccg cgaggacttt cagcggttct gggagtatca atcgccgttc tgggccggaa
     1321 agttcctgga cgagtggtgt actcgcgtca tgcgttcgcg gttgccgccg ctgaagaaag
     1381 tggcccgcag cctgcgcaag caccgccccc tgctgctcaa ctggtttcgc gcccgagggg
     1441 caatctcggc cggaattgtc gaaggtttta acaacaaggc gaaactgact atgagaaaat
     1501 cgtatggctt ccgcgagtat gaaacgatcc aactggccct ctatcaccaa cttggcgatc
     1561 tacccgagcc agaattcacc cacaaattct gctgaggagg cgaaaaacaa ttcccgggct
     1621 attgaactcc cttcccccag ttgctaccct ctatgaacca gacccacgtc tggcacagcc
     1681 cggtccaggt acctgccccg cctgcgaccg ggttttctgt tgcgcctccg agactgttgc
     1741 accctccccg ccatgcaact aagcgatctc aagtggccgc aggttgccga cctgcccaaa
     1801 g
//
LOCUS       DASZYN010000750         5409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_744692, whole genome shotgun sequence.
ACCESSION   DASZYN010000750 DASZYN010000000
VERSION     DASZYN010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744692"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VHE33_19195"
     CDS             <1..627
                     /locus_tag="VHE33_19195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99634.1"
                     /translation="AERGTPNAVRHPAPPGLRPRLARHTHLPLQSGSDTILRRMYRRY
                     RPWHYAGKLAQIRALLPDAAIGADVMIGYPGETDALFQESYDFIASQPFTYLHLFPFS
                     ARPGTPAWELHRQNPVPARAVQERMAALRELIAVKSRVFRSGFIGRTLSAITIGDDTE
                     NPGPNPTTEAVTDNFLKLTLDAPLPANRLVSARVTALWDDGLRGTPIA"
     gene            1276..2352
                     /locus_tag="VHE33_19200"
     CDS             1276..2352
                     /locus_tag="VHE33_19200"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HVT99635.1"
                     /translation="MRQRIFAISWITAALLASTATHAQNKYFQNWPENADPHVIGDRV
                     ALHFLPSPHMTPIVYPEVCAWYGALKYAEATDNTALESELQNRYTALLNPQFASLIPD
                     REHVDYEIFGVVPLQISIETHNHADQQAGLRFADRQWANPTPDGLSGETRYWIDDMFM
                     LTILQLEAYRATNDRKYLDRDANEMVAYLRKLQQPNGLFYHAPDVPFFWGRGDGWVAV
                     GMTEMLLSLPENHPQRAEILAGYRKMMAELIKNQNPDGMWRQLIDHPESWEETSSTGM
                     FTFALIEGVRHGWLDEETYGPVARKGWIGLAGFVDQNADVTNVCAGTNKQNSLAWYLA
                     RPRRTGDFHGQAPLLWAARALLEK"
     gene            2776..4149
                     /locus_tag="VHE33_19205"
     CDS             2776..4149
                     /locus_tag="VHE33_19205"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HVT99636.1"
                     /translation="MYEHIPDLLDHLPRHARSWFQNLATRPVRATATSDQLRETLGGP
                     LPEEATAPATVIDALANAGATGTVASAGPRYFGFVVGGSLPAALAADWLVSTWDQNAG
                     IFALSPLASTIEQITGSWLREIAGLSSTMSFGFVTGGHMANFTSLACARHSVLRKVGW
                     DVEANGLFGAPPVEVYVNEESHYTIFVALRLLGLGGNRVHKISADEQGRMRADLLADS
                     LRETSGPAIVCAQAGNVNTGAFDPIPAIADAAKERSAWLHVDGAFGLWAAASPHHAHL
                     VRGIERADSVATDAHKWLNVPYDCGLIFCADEAAHRTAMSLAAAYIVATSTERDPHEF
                     VPEESRRARAIPVYAALRSLGRRGLAAMIERNCRQARRFGEALRAAGFEILNDVVLNQ
                     VLVSFGTPEQTQATIAAVQNDGTCWCGGTVWKGRTAMRISVSNWSTTDEDVERSIEAI
                     IRCARIG"
     gene            4396..>5409
                     /locus_tag="VHE33_19210"
     CDS             4396..>5409
                     /locus_tag="VHE33_19210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT99637.1"
                     /translation="MLNDMVQSLLPSIYPILKSSFHLDFSQVGLLSLTYQLVASCLQP
                     FIGLYTDRRPVPYALSVGMCFTLAGIGVLAVAPTFGLLLVGAAIVGAGSAIFHPESSR
                     IARISSGGRHGFAQSFFQVGGNTGSAIGPLLAAFIVLPEGQRGVAWFAIAAAAGIFIL
                     YRVGRWRSHHLSRATPAHTQVEPRHHLPRPTIFRALAILAALIFSKYFYLASLSSYYT
                     FYLLSRFNVSVRSAQLHLFVFLGAAAAGTFFGGPIGDRIGPKHVIWGSILGVLPFTLL
                     LPHASLFWTGILSAIIGFVIASAFSAIVVYAQELMPGRVGLVSGIFFGLAFGMGGIGA
                     AVLG"
BASE COUNT         1032 a         2045 c         1343 g          989 t
ORIGIN      
        1 gccgaacgcg gaacgccgaa cgccgttcgg caccctgcac cccctggcct gcgccctcgt
       61 ttggcgcggc acacccacct cccgcttcag tccggctccg acaccatcct ccgccgcatg
      121 tatcgccgct atcgtccctg gcattacgcc ggaaagctcg cccaaatccg cgcgcttctc
      181 ccagacgccg ccatcggcgc tgacgtcatg atcggatacc ctggcgaaac cgacgccctc
      241 tttcaggaaa gctacgactt tatcgcctcg cagcccttca cctacctcca cctctttccc
      301 ttctccgccc gcccgggcac accagcctgg gagctccacc gccagaaccc cgtccccgcg
      361 cgcgccgtgc aggagcgcat ggccgctctg cgtgaactca tcgccgtgaa gtcccgcgtc
      421 ttccgttcag gattcatcgg ccgcaccctc tccgccatca ccatcggcga cgatacggaa
      481 aatcccggcc ccaacccaac caccgaagcc gtcaccgaca acttccttaa gctcaccctc
      541 gacgcaccgc tgcccgccaa tcgcctcgtc agcgcccgcg ttaccgcact gtgggacgac
      601 ggcctccgcg gcacccccat cgcatagctc cgctgcttat actggctcgg tcctcaggcg
      661 cgctccccaa aaacgctgcg ccgcctgacc tcggaggatt cccggatgcg aacgcgcctg
      721 atcgcaatca catgcctctg ccaacgaaag gcggcgcacc catcaggtcc ggaatcgttc
      781 ccgagagaag gtcaccccct tttgaagaag gccaggttca ccccacagca agcagcagac
      841 gcctttgtcc gctggatcgt ccaccacttt ccatcgcaat caaaaacaga agtacccttc
      901 caacaacgca gcgaaccatt tcggaaccgg acgaaccaac caaacccaca ccctggaaga
      961 gggccaggtt caccccacag caagcagcgg acgcctttgg tcacgggacc gtccacagat
     1021 ttccttcgca tccgcaaaca aaagtaccgc cacaccaccc tcgcgaacca ttttggaacc
     1081 gaagcggcct gcaaaacact ctccccaatg ctggagggcc aggttcaccc cacagcaagc
     1141 agcagactct attttctgct gcgacatcaa cccaataaat cccgaacacc acccgaaagt
     1201 accgaccggg aattggaacg gaccgttttg aaacgcaaca gacttcgtca caaatacact
     1261 tcggaggttt ctcgcatgcg ccagcgcatt ttcgccatct cctggatcac agcagccctt
     1321 ctcgcctcca ccgcgacgca tgcccagaac aaatatttcc agaactggcc cgagaatgcc
     1381 gacccccacg tcatcggcga tcgcgttgcg ctgcatttcc tgcctagccc acacatgacg
     1441 cccatcgtct atccggaggt ctgcgcctgg tacggcgccc tcaaatacgc tgaggccacc
     1501 gacaacaccg ccctcgaatc ggaactccag aatcgctaca ccgcactgct caatccgcag
     1561 ttcgcgagtc tcatccctga tcgcgagcac gtcgactacg aaatcttcgg cgtcgttccg
     1621 ctgcaaatct ccatcgagac ccacaaccac gccgaccagc aggccggcct tcgcttcgct
     1681 gatcgccagt gggccaaccc cacacccgac ggcctctccg gcgaaacccg ctattggatc
     1741 gatgacatgt tcatgctcac catcctccaa ctcgaggcgt atcgcgccac caacgatcgc
     1801 aagtacctcg accgcgacgc caatgagatg gtggcctatc tgcgcaaact gcagcagccc
     1861 aacggcctct tctaccacgc gcctgacgtt ccgttcttct ggggccgcgg agacggctgg
     1921 gtcgccgtcg gcatgaccga gatgcttctc tccctgcccg agaaccatcc gcagcgcgcc
     1981 gaaattctcg ccggctaccg aaagatgatg gccgagctga tcaaaaacca gaatccggac
     2041 ggcatgtggc gtcagctcat cgaccacccc gagtcctggg aagaaacctc ctccaccggc
     2101 atgttcacct tcgcgctgat cgaaggcgtt cgtcacggct ggctcgatga agaaacctac
     2161 ggccccgtcg cgcgcaaggg ctggatcggc ctcgccggct tcgtcgatca gaacgctgac
     2221 gtcaccaacg tctgcgccgg caccaacaag cagaacagcc ttgcctggta cctggcccgc
     2281 ccccgccgca ccggcgactt ccacggccag gccccgctgc tttgggcagc gcgagcgctg
     2341 ctcgaaaaat gaggcctcgc atcctcaggc aagaaacttc tttagcccca ccccgccgcc
     2401 aaacgcccag gttcacccca tagcaagcag cggactcctc cagcctccgc ctgtccaccg
     2461 aatggactcg gcgccgcaaa ccaaagtacc gcccgccgaa tcgcaacgaa ccatttcgga
     2521 accagaccag agaaacctaa accccggacc caggagacgg ccaggttcac cccatagcaa
     2581 gcagcggact cctccagcgc ctgcctgtcc accaaattgc tgccggacgg caaacgaaag
     2641 tacgtcccgg gcatctccag ggaccgtttc ggaacgaaac cgatcagcag cccgaacgaa
     2701 ccggcacaag cccatcaacc ccattcgctg cacccgcatt gcccgcgcac gccccgccca
     2761 gcgaagatag tcgccatgta cgaacacatc cccgatctgc tcgatcacct gccccgccac
     2821 gcccgctcgt ggtttcaaaa cctcgccact cgtccggtcc gagccaccgc gacctccgat
     2881 caactccgcg aaaccctcgg cggcccactc cctgaagaag ccaccgctcc cgcgaccgtc
     2941 atcgacgccc tcgccaacgc tggagccacc ggcaccgtcg cctctgccgg acctcgctac
     3001 ttcggtttcg ttgtcggcgg cagcctccct gccgccctcg ccgccgactg gcttgtttcc
     3061 acctgggatc agaacgccgg catcttcgcc ctctcgcctc tcgcctccac catcgaacaa
     3121 atcaccggct cctggctacg cgaaattgcc ggcctttcct ccaccatgag cttcggcttc
     3181 gtcaccggcg gacacatggc caacttcact tcactcgcct gcgcgcgcca cagcgtcctg
     3241 cgcaaggttg ggtgggacgt cgaagccaat ggcctcttcg gtgcaccgcc cgtcgaggtt
     3301 tacgtcaacg aggaatcgca ctacaccatc ttcgtcgcgc tccgccttct cggcctgggt
     3361 ggcaaccgcg tccacaaaat ctccgccgac gaacagggcc gcatgcgtgc cgaccttctc
     3421 gcagactccc tgcgcgaaac ctccggaccg gccatcgtct gcgcccaggc aggcaacgtc
     3481 aacaccggcg ccttcgaccc aattcccgcc atcgccgacg ccgccaaaga acgcagtgcg
     3541 tggctccacg tcgacggcgc tttcggactc tgggccgctg cctcgccgca ccacgcgcat
     3601 ctcgtccgcg gcatcgaacg cgccgactcc gtcgccactg acgcgcacaa atggctcaac
     3661 gtcccctacg attgcggcct gatcttctgc gccgacgaag cagcccatcg caccgccatg
     3721 tccctcgccg ccgcctacat cgttgccacc agcaccgagc gcgatcctca cgaatttgtc
     3781 cccgaagaat cgcgccgcgc ccgtgcgatc cctgtctacg ccgcacttcg ttccctcggc
     3841 cgccgtggcc tcgccgcaat gatcgagcgc aactgccgcc aggcccgccg cttcggcgaa
     3901 gctctccgtg ctgccggttt cgaaatcctg aacgacgtcg ttcttaatca ggttctggtt
     3961 tccttcggaa cgcccgaaca aacccaggcc acgatcgccg ctgttcagaa cgacggaacc
     4021 tgctggtgcg gtggcaccgt ctggaaaggc cgcaccgcca tgcgcatcag cgtcagcaac
     4081 tggtcgacta cggatgaaga tgtggagaga agtatcgaag caatcatccg ctgcgcccga
     4141 attggctgac gttcaaaaac gctatccgct gaacgcttgc gctgttgacc tggatctacg
     4201 cacgcgccat agattcatcg aacaggcacc ggcaagtcca cctatatacg atggaataac
     4261 cttcgccatc ccaccatgaa aatggcttgc ccaactcccg cgatgtcccc tggccccggt
     4321 gaagtgcccg cgcctcggcc gtccgctgca gcgacgggcg catttcgcat actcgctgca
     4381 gtaagcctct gccacatgct caacgacatg gtgcagtcgc tgctgccctc gatctatccg
     4441 atcctcaaga gctccttcca cctcgacttc agccaggtgg gactgctctc tctgacctat
     4501 cagttggtag ccagttgcct gcagcctttc atcggccttt acacggaccg ccgtcctgta
     4561 ccctacgcac tttccgttgg catgtgcttc actctggctg gcatcggggt gctggccgtt
     4621 gcacccacct tcggactgct cctcgtcggt gcagcaatcg tcggtgcggg ctctgccata
     4681 ttccaccccg aatcgtcccg catcgcgcgc atctcttccg gtggccgcca cggcttcgca
     4741 cagtcgtttt tccaggtcgg aggaaatacc ggcagcgcca tcggccccct gctcgccgct
     4801 ttcatcgtgt tgcctgaagg ccagcgcggt gtggcgtggt ttgccattgc cgcggcggcg
     4861 ggaatattca ttctctatcg cgtcggccgg tggcggtcgc accatctctc ccgggccacg
     4921 ccggcacaca cgcaggtcga accgcgccat caccttccgc gccccaccat cttcagggca
     4981 ctcgcaatcc tggccgcgct gatcttctca aaatatttct atctcgcaag cctctcgagc
     5041 tactacacgt tttacctgct cagccgcttc aacgtatcgg tgcgcagcgc gcaattgcac
     5101 ttgttcgtct tcctgggagc cgcggccgcg ggaacgtttt tcggagggcc gatcggcgac
     5161 cgcatcggtc ccaagcatgt catctggggt tcgattctcg gcgtgctccc ttttacgctt
     5221 ctcctgccgc acgctagtct tttctggacc ggcatcctct cggcgatcat tggctttgtg
     5281 atcgcctcgg cattttctgc cattgtggtt tatgcgcagg aactgatgcc gggccgcgtt
     5341 ggccttgtct ccggcatctt cttcgggctc gcatttggca tgggcggaat cggcgcggcg
     5401 gtgctgggc
//
LOCUS       DASZYN010000751         4799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_52474, whole genome shotgun sequence.
ACCESSION   DASZYN010000751 DASZYN010000000
VERSION     DASZYN010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52474"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1279)
                     /gene="metX"
                     /locus_tag="VHE33_19215"
     CDS             complement(158..1279)
                     /gene="metX"
                     /locus_tag="VHE33_19215"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HVT99638.1"
                     /translation="MSTETQNVVKAAAAIQPDFEGDYVIDEVVPLESGEVLVRPTQHY
                     AIYGKLNAARDNAVFVAHALSGNAEVATWWPQLFRKPEAMLDLDRDCVIGINIFGSCY
                     GSTGPGSVDPETGRLYGPTFPLINVRDMVRAQEKLITSLGIKKIKLAIGASIGGMQVL
                     EWAVLFPDRVSRAISIGAAPLGAMGLGLNHLQRQAIMLDPKWAGGYYLPDDEPRGGLA
                     IARALGVISYKSVPLFEARFGRKPNRTGENPWISTAAGQNGRFDIAGFLDYQGHKFNE
                     RFDANAYISITRAMDTFDPVRAHASPLAAYSRIQAHVTMVGISTDWLFPPEDVRSMAV
                     VMAAAGARCDYREMASNHGHDAFLAEPEHLVRLLTPVFE"
     gene            complement(1324..2640)
                     /locus_tag="VHE33_19220"
     CDS             complement(1324..2640)
                     /locus_tag="VHE33_19220"
                     /inference="COORDINATES: protein motif:HMM:NF006096.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine synthase"
                     /protein_id="HVT99639.1"
                     /translation="MAEEQKQHLATLAVHGGQTPDPTTGSRAVPIYQTTSYVFQDADH
                     AARLFGLQEFGNIYTRIMNPTTDVFEKRVAALEGGAAALATASGQAAETLAITTLANA
                     GDEIVSTTSLYGGTYNLFHYTLPKLGITVKFVDADDFDGLRKAITPKTRAVYTETLGN
                     PKLDICDVETLAKIAHENGLPLIIDNTSVTPILLRPIEYGADIVVESATKYIGGHGNS
                     IGGIIVDAGKFDWKASGRFKDFVEPDPSYHGLSFVEAFGPLAFILKARVQGLRDTGAA
                     LSPFNAWLFLQGLETLHLRMQRHSQNALAVAEHLEKHAGVEWVNYPGLQSSKYAARAK
                     KYLPDGASGLITFGIRGGFDAGKKFINSLKIFSLLANLGDAKSLVIHPASTTHQQLSG
                     EEQKSTGVLPEMVRLSVGIEDVRDLIADIDQALEVAVPSPELVGAR"
     gene            3130..3516
                     /locus_tag="VHE33_19225"
     CDS             3130..3516
                     /locus_tag="VHE33_19225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99640.1"
                     /translation="MMVAVDLVDRWLASQGLHAEVTRFDDILPGLVAAALVFLIQRQQ
                     NRELRRQRQNAAVIDQMNHHIRNALQVIVARASLDHEATPELRQIDDAVARIDWALRE
                     ILPQSATDKPIPEREPERREAAATPH"
     gene            complement(3807..4427)
                     /locus_tag="VHE33_19230"
     CDS             complement(3807..4427)
                     /locus_tag="VHE33_19230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99641.1"
                     /translation="MAAMQGQGANRLIAGHFGFAAIVKSREPRVPLWSLMLATVWLDI
                     VFVPLFLLGIETLRPAPGLQGGYGQYIIYADYTHSLLGALALSAAFGLMFSWLWGRRC
                     AVVLGFVSFSHWLLDLLVHRGDMPILPGNSGNLPRLGFGLWQFRTGSIVAELLLLTAG
                     ALCYWRAARAVTAAAHRGRTRAAITAMLIFLCGVVVLTLDVTGWPG"
BASE COUNT          934 a         1684 c         1310 g          871 t
ORIGIN      
        1 accattcgca ggcagcccgt gaccagcacc gacacgctaa ccctgcatct ggtcaccggc
       61 ggcggctgtg ccatccgctt tgtgccggaa tcatagacgt gagccctgtg ccctgtgccc
      121 tgtgccctga gccctgcgct ctatgccctg cgccctgtta ctcaaacacc ggcgtcagca
      181 accgcaccaa atgctccggc tccgccaaaa acgcatcgtg cccgtggttt gacgccatct
      241 cccggtagtc gcatcgcgcg ccggccgcag ccatcaccac cgccatcgat cgcacatcct
      301 cgggcggaaa cagccagtcc gtcgaaattc ccaccatcgt cacatgcgcc tggatgcggc
      361 tgtacgcggc caggggcgaa gcatgcgcgc gcaccggatc gaacgtatcc atcgcccgcg
      421 tgatcgagat gtacgcgttc gcgtcgaacc gctcgttgaa cttgtgtccc tgataatcca
      481 ggaatcccgc aatatcgaac cgcccgttct gtcccgccgc tgtcgagatc cacggattct
      541 cgccggtccg gttcggcttg cgcccaaagc gcgcctcaaa cagcggcacg cttttgtagc
      601 tgatcacgcc cagcgctcgt gcaatggcca gcccgccgcg tggctcatcg tccggcagat
      661 aatacccgcc cgcccacttg ggatcgagca tgatcgcctg ccgctgcaga tgattcagcc
      721 ccagtcccat cgcacccagc ggcgccgccc caatgctgat cgcccgcgag acccgatccg
      781 gaaacagcac cgcccactcc agcacctgca tcccgccaat cgaggcgccg atggccagct
      841 tgatcttctt aattccgagc gacgtgatca gcttttcctg cgcccgcacc atgtcgcgca
      901 cattgatcag cggaaacgtc ggaccataga gccgtcccgt ctccggatcg acgctccccg
      961 gcccggttga cccatagcag gaaccaaaaa tgttgatccc gatcacgcag tcccgatcga
     1021 gatcgagcat cgcctccggc ttcctgaaca gctgcggcca ccacgtcgcc acctccgcgt
     1081 tgcccgacag cgcgtgtgca acaaagactg cgttgtcccg ggcagcattc agcttgccgt
     1141 agatggcgta gtgctgcgtc ggccgcacca gcacctcgcc gctctcgagg ggaacgacct
     1201 cgtcgatgac gtagtccccc tcgaaatccg gctggatggc cgccgcggcc ttcacaacat
     1261 tttgcgtttc cgtgctcatt gtttagctga ctcgctaacc tgctgactcg ctgacgagct
     1321 gcttcaccgt gccccaacca gctccggact cggcaccgcc acctccagtg cctggtcgat
     1381 gtccgcaatc agatcgcgca catcttcgat ccccaccgag agccgcacca tctccggcag
     1441 cacgcccgtc gacttctgct cctcgccgct cagctgctgg tgcgtcgtcg atgcaggatg
     1501 gatcacgagc gacttcgcat cgcccagatt ggccagcagg gagaagatct tcagcgagtt
     1561 gatgaacttc ttgcccgcat cgaatccgcc ccggattccg aaggtgatca gcccgctcgc
     1621 gccgtccgga agatacttct tcgcccgcgc cgcatacttg ctcgactgca ggcctggata
     1681 attcacccac tccacgcccg cgtgcttctc cagatgctcg gccacggcga gggcattttg
     1741 ggaatgccgc tgcatgcgca gatgcagcgt ttcgagcccc tgcagaaaca gccacgcatt
     1801 gaagggcgac agtgccgcgc ccgtatcgcg cagcccctgc acgcgcgctt tcaggatgaa
     1861 cgccagcgga ccgaaggcct ccacgaacga cagcccgtga tacgacggat cgggctcgac
     1921 aaaatcctta aaccgccccg acgctttcca gtcgaacttc ccggcgtcca cgatgatccc
     1981 gccaatcgaa ttcccgtgcc caccgatata ctttgtcgcc gactcgacga cgatgtccgc
     2041 tccgtactcg atcggccgca gaagaatcgg cgtcacgctg gtgttgtcga tgatcagtgg
     2101 cagcccgttc tcgtgcgcga tcttcgcgag cgtctccaca tcgcagatgt cgagctttgg
     2161 attccccagc gtctccgtgt acaccgcccg cgtcttcggc gtgatcgcct tgcgcagccc
     2221 gtcaaagtca tcggcgtcga cgaacttcac cgtgatgccc agcttcggca gcgtgtagtg
     2281 gaacaagttg tacgtcccgc catacagcga cgtcgtcgac acgatctcat cgcccgcgtt
     2341 cgccagcgtc gtaatcgcca gcgtctccgc cgcctgtccc gacgcggtcg ccagcgccgc
     2401 cgctccgcct tcgagggccg cgacgcgctt ctcgaataca tccgtcgtcg gattcatgat
     2461 gcgcgtgtag atgttcccaa actcctgcag tccgaacagc cgcgccgcat ggtccgcgtc
     2521 ctggaacaca tacgaagtgg tctgatagat cggaaccgcg cgcgacccgg tcgtcggatc
     2581 gggagtctgt cccccgtgca ctgccagtgt cgccagatgc tgtttctgtt cttccgccat
     2641 tgcttcctct ccttcgctgc ctgctaattc gccgacctgc tgactcgctc cactgcgccc
     2701 tgcgctctga gccctgtgct ctgcaaacac aaacggcccg cgatctttga ccgtaggccg
     2761 ctggaaacct ttcgaactcg ctttactctg cttgttcgct cccgcgcacg gtctcgccgc
     2821 acatgcccat acacaggccc atcgtgagcg cagcgccgca gttgtggcgg gagttcatcc
     2881 cagtcagtat cagggtctgc ttcagctttc gtcaaaccaa acgcggagtt gtcatccgct
     2941 tgtcatagca ttttgtgggc gttcccaccg gtcactcgac cggaagcagc acctgatccg
     3001 cgcgaagaat ggaagcgatt cgcccgaccg attggcctgt tgctgcgtct aatgattgca
     3061 acgagcaaat cgggagtacg aagaatgtcc tggcgccggt ttggaatccc agccatatcg
     3121 ggcgtggcca tgatggtcgc cgtcgacctt gtggaccgct ggctggcctc gcagggactg
     3181 cacgcggaag tgacgcgctt tgacgacatc ctgcccggtc tggtcgcagc cgcgctggtt
     3241 ttcctcatcc agcgccagca gaatcgagaa ctgcgccggc agcggcagaa tgctgccgtc
     3301 atcgatcaga tgaaccacca catccgcaac gcactgcagg tcatcgtcgc ccgcgccagc
     3361 ctcgatcacg aggccacccc cgagctgcgc cagattgacg acgccgtcgc ccgcatcgac
     3421 tgggccctgc gcgaaatcct gccacaaagc gccaccgaca aacccatccc cgaacgcgaa
     3481 cccgagaggc gcgaggccgc cgccacccca cactgatccg ccctctcccg gacggaggat
     3541 ggtcgcgaaa cctgtggaac gccggtgacc cacgcgcaac ctgggcaacg ttcgctgcgc
     3601 attgggtgcg gcggagcggc gcgacgtgaa gccccggcct tcatttcgcc tgccggaagg
     3661 ccgatcaggt tggtcccgct cttcgaaaaa actgccggtt tgccggtctt acggctgcga
     3721 cgacacggca gtgtcggaaa ccgtcgatga gtggtttaag atttgccatt caccggatgc
     3781 gcatccgggt caacagaatg ttcggatcat cccggccatc cggtgacgtc cagcgtcagt
     3841 acgaccacgc cacaaagaaa aatgagcatt gccgtaatcg ctgcccgggt acggcccctg
     3901 tgcgcggcgg ccgtgacagc gcgcgcggcg cgccaatagc acagcgcacc cgccgtcagc
     3961 agaagaagct ccgccacgat cgaacccgtt ctgaactgcc acaaaccgaa gccgagtctc
     4021 ggcagattac cggaattgcc gggcaggatc ggcatgtcgc cacggtgaac gagcagatcg
     4081 agcagccagt gagaaaagga gacgaatccc agaacgacgg cgcagcgcct gccccacaac
     4141 cagctgaaca tcagaccgaa tgcggcggag agtgccagcg cgccaaggag cgagtgcgtg
     4201 tagtcggcgt agatgatgta ttggccataa ccaccctgga ggccaggcgc aggcctcagt
     4261 gtctcgatcc ccaacagaaa gagcgggacg aagacgatgt cgagccatac tgtcgcgagc
     4321 atcagtgacc atagcggtac ccttggttcg cgcgacttca cgattgcggc aaaaccgaaa
     4381 tgcccggcga tcaagcggtt ggccccctgg ccctgcatcg cggccacggt acacccgcat
     4441 cgagcagccc caaaacaact acgaacagaa tgactccaag aatgaattgc ataatcttcc
     4501 gctggaacgt tgagcttctc cgcgctcgag cccgtcgaca aagccgaaca ggttctggct
     4561 ccgtccgtga acaggctgtg aaatcgggta aagcacgcca gatctcgttg ccttcgaatt
     4621 atcgaaaact gagggtacca aaccgctgag ccagtcacca gcgtgtcacc aatgagtagt
     4681 cagctacacc gacgcttggc cctccgtctc aggccccgaa actgtatccg caccgccacc
     4741 tccggccact accgcgaatg cgccgcccaa acctcccccg ccagcactcc cgctgagat
//
LOCUS       DASZYN010000752         6629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_955798, whole genome shotgun sequence.
ACCESSION   DASZYN010000752 DASZYN010000000
VERSION     DASZYN010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955798"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VHE33_19235"
     CDS             complement(<1..197)
                     /locus_tag="VHE33_19235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99642.1"
                     /translation="MKCPYCGFGQDRVIDSRESREADSIRRRRECEKCNRRFTTYERI
                     DEIPYMVAKKDGRREKFERRKV"
     gene            329..796
                     /locus_tag="VHE33_19240"
     CDS             329..796
                     /locus_tag="VHE33_19240"
                     /inference="COORDINATES: protein motif:HMM:NF018925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HVT99643.1"
                     /translation="MSTTPRKRSITKGLLAGLVGGLAGAGAKMVAEQVFPPRTQGQTP
                     PPVVLAENVTGRRLPEGERELALQGIHWIFGAVAGAVYGAMVEVEPSLGAWRGAAFGI
                     TLNRITHESILPKLGLTAPPGRQPTQERISEWVTHAVYGVATDSVRRAVRRAL"
     gene            880..1713
                     /locus_tag="VHE33_19245"
     CDS             880..1713
                     /locus_tag="VHE33_19245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HVT99644.1"
                     /translation="MPARPTWSGSIQISLVSIAVRIFPASNPARSVEFHQIDRRTHQR
                     VHHRNVDESGEVEKSDIVKGYEYARNQYIEIDPDELKALRLATATTMQIQQFVKTEEI
                     PEALFDRPYFVAPKDAAQAKALAIMRKALDETGTCGIGEVAFSGREHLVALAAPPDPK
                     QKGLMLYLLRYEEELRDAKSVLGDIKEATATSQEVSLAKQLIEGSTSKFDLSAYKDDY
                     EAAVVKMVNAKRKGKPLPEPEPAAPKGKVVNIMDALRKSLAEHEHPAKKAKSTRRKKA
                     A"
     gene            complement(1739..2611)
                     /locus_tag="VHE33_19250"
     CDS             complement(1739..2611)
                     /locus_tag="VHE33_19250"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005091.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HVT99645.1"
                     /translation="MARIAIVGVGSIGGAIAGFLESAGGHDILLCTRRPIEQLTVTMP
                     SGLVRVQARNVTAPEKAEPVEWVIVATKAYDAESASRWFPALRTGGAAVAVLQNGVEH
                     RERFAPYVEPERIVPVVIDCPAERRADGDVHVRGPASMRLEDTPTGRAFTALFAGSSV
                     KVELVADFITAAWRKLCLNSVGALNALTLKPAGLLRDETVASLALQIVAECVAVGRAE
                     GAQLPDSVGDEVLAHYRAQPPDAVNSLLADRLAGRPMEIDARNGVIVRRGEKHGIPTP
                     LNRMAVALLTAQQS"
     gene            complement(2681..3763)
                     /locus_tag="VHE33_19255"
     CDS             complement(2681..3763)
                     /locus_tag="VHE33_19255"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HVT99646.1"
                     /translation="MKSNRTRLVALVIVVALAVAIWHERHRIHFDWHVFGQQLRMADW
                     RRIAIALACIYAGYLVRSVRWAWLIRHKKKTPLLSLLGTQVMGFTAVALIGRVADLVR
                     PYLVAKKTGLPLSEQMAVYVVERLFDAGSMALITSSAILLAPPGALPHPEVLKRAALF
                     GLLLTGAGGLFLLAVRLWGEGVAHFSNRAFGLLSRRAGEAVKDKIQAFHAGLDTIRTP
                     GDFAAVAACSLGMWMLIAVSYLETMLAFTASPELASISPAKGVLMMVVSGGASVIQLP
                     IVGWFTQIGLVAGAISKFFGVAPEAATGCAATLLLVTFLAIVPVGLIWSRFEHISLKQ
                     VTAESEHAGEELVHHHGSVGEKATSG"
     gene            complement(3801..4511)
                     /locus_tag="VHE33_19260"
     CDS             complement(3801..4511)
                     /locus_tag="VHE33_19260"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HVT99647.1"
                     /translation="MRRFGRALGCVWLAASFAATAAYAAERVTLKNGFDLICDHRVPE
                     GAKARLYMDAAGASYIEVNASDIVSGEPIDLAKPATAAVKPAPQPALPEKLTDADLQP
                     MLAKAGTAYNLDADLLASVVRAESAGHSHAVSHAGAQGLMQLMPGTASDLGVGDTFAP
                     KDNIQGGTAYLDWLLKRYNDNLQLALAAYNAGPAAVDRWHGIPPYRETRLYVARVIRE
                     YNRRYLQRHQAELRAAAR"
     gene            complement(4558..5493)
                     /locus_tag="VHE33_19265"
     CDS             complement(4558..5493)
                     /locus_tag="VHE33_19265"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HVT99648.1"
                     /translation="MTALIRMIVVDIDGTLLGSPNSAISERNCRALCEAQTAGIDIVI
                     ATGRRQAYAEPLLSPIGLNPDTVCITSNGTVTRTLAGRRIDRFLLPVETALALCPALR
                     PLGTTVFTFDHDGPGELVLESVEALEGRVSMWVEANRPWIREVRPLERAFDTGQAPVQ
                     SMVCGPVEDMRRAEEWLVESDLSASIELHRTEYPSRDLTILDILPPGCSKGAALRRLA
                     KQRELGPEEVMAIGDNWNDLEMLEFAGRPVVVTNGAPDLVELARRRKWEIAPSADEDG
                     VGQVIGELLAGRGSPSESEAVVNGTSVTAYSPERS"
     gene            complement(5520..5969)
                     /gene="tadA"
                     /locus_tag="VHE33_19270"
     CDS             complement(5520..5969)
                     /gene="tadA"
                     /locus_tag="VHE33_19270"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HVT99649.1"
                     /translation="MSTDETLMSAALQEAKAAAEAGEVPIGAVAVLGGEIIARGQNRV
                     LRDVDPTAHAEIIAMRAAARAIGNYRLVDCDLFVTLEPCAMCAGAMIHARLGRLVYGA
                     ADPKAGAAGSVLGVLNHPRLNHQMNVTCGVLSEECGEVLREFFQKRR"
     gene            complement(5966..>6629)
                     /locus_tag="VHE33_19275"
     CDS             complement(5966..>6629)
                     /locus_tag="VHE33_19275"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HVT99650.1"
                     /translation="NLLGLPRWEPTIETAAGLHRVVLEGGSIDVNGAGTLITTEECLL
                     SNVQQRNPGVSREQLEKAMHDYLGIDQVLWMNRGIAGDDTHGHVDDITRFVGSNTIVT
                     VIEPNAADDNHLPLQENLERLQAARNLDGKPFEVVELPMPPPIVFQGQRLPASYANFY
                     IANGLVLVPTFNRATDRNALNILAETFPSREVIGIYSGDLIWGLGTLHCMTQQQPKAD
                     AP"
BASE COUNT         1251 a         2302 c         1895 g         1181 t
ORIGIN      
        1 accttgcggc gctcgaattt ctcccgccgg ccgtccttct tggccaccat gtagggaatt
       61 tcatcgatgc gttcgtaggt cgtaaagcgg cggttacact tctcgcattc gcgccgcctg
      121 cggattgagt cggcttccct gctctcccgc gagtcgatca cgcggtcctg gccaaacccg
      181 caatagggac atttcatcgc tcggtttcgt cgctcagctt tcctggctct ggtttcccgg
      241 ctctggttcg attctagcag cgacacaatt gtctgctgta cgctccacgc tgaacgctgt
      301 accctgttcg cgacgcatct caccccatat gagcacgact ccacgcaaac gcagcatcac
      361 caaaggcctg ctcgccggtc tggtcggcgg actcgccgga gccggcgcca agatggtcgc
      421 cgaacaggta ttcccaccgc gcacccaggg ccagacaccg ccgccagtcg ttctcgccga
      481 gaacgtcacg ggccgccgcc ttcctgaagg cgagcgcgag cttgccctgc agggcattca
      541 ctggatcttc ggcgctgtcg ctggcgcggt gtacggcgcg atggtcgaag tcgaaccttc
      601 tctgggggcc tggcgcggcg ccgctttcgg gatcacgctg aatcgcatta cgcacgagtc
      661 catccttccc aagcttggcc tcaccgcccc tcccggacgc cagcccacgc aggagcgcat
      721 cagtgagtgg gttacccatg ccgtctacgg cgtggcgacc gacagcgtcc gccgcgccgt
      781 ccgccgcgct ctgtaatcga ggagcaacga tccactggtc aggaagtcgc gtgcggccct
      841 cagcgcatcg taatttgtag gtcattgcaa ggagctacga tgcccgcacg ccctacctgg
      901 tccggttcca tccagatctc gctcgtctcg attgccgtcc gcattttccc cgcttccaac
      961 ccagcccgtt cggtcgaatt ccatcagatc gaccgccgca cccatcagcg cgtccaccac
     1021 cgcaacgtcg acgaatccgg cgaggtcgaa aagagcgaca tcgttaaagg ctacgagtac
     1081 gcgcgcaatc aatacatcga gatcgatccc gacgagctga aggccttgcg cctggccact
     1141 gcgaccacca tgcagattca gcagttcgtg aagacagaag aaatccccga agccctcttc
     1201 gatcgccctt atttcgtcgc gcccaaagac gccgcccagg ccaaggccct ggccatcatg
     1261 cgcaaggcgc tcgacgaaac cggcacctgc ggcattggcg aagtcgcctt cagcggccgc
     1321 gagcacctcg tcgccctcgc cgcccctccc gatcccaaac agaagggcct catgctctac
     1381 ctgctccgct acgaggaaga gctgcgcgat gcgaagtccg tcctgggcga catcaaggag
     1441 gcaacggcca cttcccagga agtctccctc gccaaacagc tcattgaggg cagcacgtcg
     1501 aaattcgatc tctccgccta caaggacgac tacgaagccg ctgtcgtgaa gatggtcaac
     1561 gcgaagagaa aaggcaagcc actgccggaa cctgagccgg ccgcgccaaa gggaaaggtc
     1621 gtcaacatca tggatgccct gcgtaaaagc ctcgcggagc acgagcatcc tgcgaagaaa
     1681 gcgaagagca cgcggcgaaa gaaagccgct tgaaacgggc agcagtctcg cggcccggct
     1741 agctctgttg cgccgtcagc agcgccacgg ccatacggtt cagtggcgtg gggatgccgt
     1801 gcttctctcc cctgcgcacg atgacgccat tacgggcgtc aatctccatg gggcgtccgg
     1861 caagacggtc ggcgaggagc gaattgacgg cgtcaggcgg ctgcgcacgg tagtgggcga
     1921 gaacttcgtc gcccaccgag tcgggaagct gggctccctc agcgcggccg accgccacgc
     1981 actcggcgac gatttgcagg gccaggctgg cgacggtctc gtcacgcagc agacccgccg
     2041 gcttgagcgt gagcgcattc aacgcaccca ccgagttcag gcacagcttc cgccacgctg
     2101 cggtgatgaa gtcggccacc aattccacct tcaccgagct tcccgcgaac agcgccgtaa
     2161 acgcgcgccc tgtcggcgtg tcttccaggc gcatgctggc gggtccgcgc acatgcacat
     2221 cgccgtccgc ccttcgctct gcagggcagt cgatcaccac gggcacaatc cgctccggct
     2281 caacgtaagg cgcaaatcgc tcgcgatgct cgactccgtt ctgcaaaacg gcaaccgctg
     2341 cgccaccggt gcgcagagcc gggaaccatc ggctggcgct ttcggcatcg taggctttgg
     2401 tcgctacgat cacccattcc acgggttcag ctttttccgg agccgtgacg ttgcgcgcct
     2461 gaacgcgcac caacccggaa ggcatcgtga ccgtcagctg ctcgatcggc ctccgcgtgc
     2521 acaggaggat gtcgtgaccg ccggccgatt ccagaaatcc ggcgattgct ccgccaatag
     2581 atcccacgcc tacgatcgca atgcgcgcca tgcgcccctc acacgccctt ccaaagggag
     2641 ttcacgcttg ctctgccggc ccgaaccatc acccggccag ctatccgctc gttgctttct
     2701 cgcccactga gccatgatga tgcaccaatt cctcaccggc atgctcgctc tccgcagtca
     2761 cctgcttcag cgagatgtgc tcgaaacgcg accagatcag ccccaccggc acgatcgcca
     2821 ggaaggtgac cagcaacagc gtcgccgcgc agcccgtcgc cgcctccgga gccacgccga
     2881 agaacttcga aatcgccccg gccaccagcc cgatctgcgt gaaccagccc acgatgggca
     2941 gctgaatcac gctggctccg ccgctgacca ccatcatcag gacgcccttg gccggcgata
     3001 tcgacgccag ttccgggctc gcggtgaagg ccagcattgt ttccagatac gaaacggcaa
     3061 tcagcatcca catgcccagc gagcatgcgg caacggctgc gaagtcgccg ggcgttcgga
     3121 tggtatcgag gccggcgtga aaggcctgga tcttgtcttt caccgcctct ccggctcgcc
     3181 tggagagcag gccaaatgcg cggttggaga aatgcgccac tccctcaccc cacaaccgca
     3241 cagcaaggag aaaaaggcct cccgctccgg tcagcagcag cccaaacagc gcggcgcgct
     3301 tcaggacctc aggatgcggc agcgcacccg gcggcgccag cagaatggcg gatgaagtga
     3361 tcagggccat cgatccggcg tcgaagagcc gctccacgac gtacacagcc atctgttcgc
     3421 tcagcggcag ccccgtcttc ttcgcgacca ggtacgggcg caccagatct gccacgcgcc
     3481 cgatcagcgc taccgccgta aagcccatca cctgcgtccc gagcagcgac agcagcggcg
     3541 tctttttctt gtgccggatc aaccacgccc agcgcaccga gcgcaccaga tagccggcgt
     3601 agatacaggc cagcgcaatg gcaatccggc gccagtcggc catccgcaac tgttgaccga
     3661 aaacatgcca gtcgaagtga atccgatggc gctcgtgcca gatcgccacg gccagcgcca
     3721 cgacgatgac gagcgcaacg agccgggttc ggttcgattt caaatcgtta ctccgggaat
     3781 gtggaacagg agcgtcagct tcaccgcgcc gcggcccgca actccgcctg atgccgctgc
     3841 agatagcggc gattgtattc acgaatcacg cgcgcgacat acagccgcgt ctcgcgatac
     3901 ggcggaattc cgtgccagcg atccaccgct gccgggccgg cgttgtaggc cgccagcgcc
     3961 agctgcaggt tgtcgttgta gcgcttcagc agccagtcga gatacgccgt cccgccctga
     4021 atgttgtcct ttggcgcgaa cgtatcgcca acgccgaggt ctgacgccgt tcccggcatc
     4081 agctgcatca gaccctgcgc tcccgcgtgg cttacggcgt gcgaatgacc ggcactttcc
     4141 gcacggacta cgctggcaag cagatcggca tcgaggttat acgcggttcc ggccttcgcc
     4201 agcatcggct gcaaatcggc gtcggtcagc ttctccggaa gcgctggctg cggtgccggt
     4261 ttcacggccg ccgtggcagg tttggccaga tcaatgggct cgcccgagac gatgtccgag
     4321 gcattcactt cgatgtaact cgccccagcc gcgtccatat acaggcgcgc cttcgccccc
     4381 tcagggacgc ggtgatcgca gatcaaatcg aagccattct tcaacgtgac gcgctcggcg
     4441 gcgtacgcgg ccgtggccgc gaacgaagca gccagccaga cgcaccccaa cgctctgccg
     4501 aatcggcgca ttcctcttta ctctaccatc ctcagacgca gggattgcgc ggtccgctca
     4561 gcttcgctcg ggtgaataag cggttacgga tgtgccattt acaaccgcct ccgactctga
     4621 tggagagccg cgtccggcga gcaattcccc gatcacctgc cccacaccat cctcatcggc
     4681 cgatggtgcg atttcccact tgcgccgtcg cgccaactca accagatcgg gagcgccatt
     4741 ggtgacgacg accggccgtc ccgcgaattc cagcatctcc agatcgttcc agttgtcgcc
     4801 gatcgccatc acttcttccg ggcccagttc ccgttgcttc gccagccgcc gcagggccgc
     4861 acctttcgag cagcccggcg gcagaatgtc gaggatggtc agatcgcgcg acggatactc
     4921 cgtccgatgc agctcaatcg acgcggacag atcgctctcg acgagccact cttcggcgcg
     4981 acgcatgtcc tcgaccgggc cgcagaccat gctctgcacc ggtgcctgac cggtgtcgaa
     5041 cgcgcgctcc agggggcgga cctcacgaat ccatggccgg ttcgcctcca cccacatcga
     5101 gacgcgcccc tccagtgctt ccaccgattc gagaacgagt tcgcccggcc cgtcgtgatc
     5161 gaaggtgaag acggttgttc ccagagggcg caacgccggg cacagcgcca gcgccgtttc
     5221 caccggcagc aggaacctgt cgatccgcct gcctgcaagt gtccgcgtca ccgtgccatt
     5281 cgacgtgatg cagactgtgt cagggttcag cccgattggc gaaagcagcg gctccgcgta
     5341 cgcctggcgc cgccctgtag caatcacgat gtcgattccg gccgtttgcg cctcacacag
     5401 ggcgcggcaa ttgcgctcgc tgatggcgga gttgggagag ccgagcagcg tgccgtcgat
     5461 gtcgacgacg atcatccgga tcaacgcagt catctgcctt tcagtctatg gcacccggct
     5521 caacgccgtt tttggaagaa ttcccgcaac acttcgccac actcctccga cagaacgccg
     5581 caggtcacgt tcatctggtg attgagcctg ggatggttga gcacgccgag caccgacccg
     5641 gccgcgcccg ctttcggatc tgctgctccg tagaccagcc gccccagccg ggcgtgaatc
     5701 atcgctcccg cacacatggc acagggctcc agagtgacga acaggtcgca atccacgagg
     5761 cggtagttcc cgattgccct ggccgcggcg cgcatcgcaa taatctcggc gtgcgccgtc
     5821 ggatccacat cgcgcaggac acgattttga ccccgcgcga tgatctctcc cccaaggacc
     5881 gcaacagcgc caatgggaac ttcgcccgcc tcggccgcag ccttcgcttc ctgcagggcc
     5941 gcactcatca gcgtttcgtc cgtgctcacg gtgcgtcggc cttcggctgc tgttgggtca
     6001 tgcagtgcag cgtgcccagc ccccagatca aatcgccact gtagatgccg ataacttctc
     6061 gcgatgggaa tgtctctgcc aggatgttca gcgcgttccg atccgttgct cgattgaatg
     6121 tcggcaccag aacgaggccg ttcgcaatgt agaaattggc atagctggcc ggcagacgct
     6181 gaccctgaaa cacaatcggc ggcggcatgg ggagttcgac gacctcaaac ggctttccat
     6241 cgaggttccg cgctgcctgc agccgctcca ggttttcctg caacggcaaa tgattatcat
     6301 ccgccgcatt tggctcaatc acggtaacaa ttgtgttgga tccaacgaat cgtgtgatgt
     6361 catcgacgtg cccgtgcgtg tcgtcgccgg cgattccgcg attcatccac aacacctgat
     6421 cgattcccag atagtcgtgc atggccttct caagctgttc gcggctcacg ccgggatttc
     6481 gctgctgtac gttgctgagc aggcactctt cggtcgtgat cagcgtgcct gcaccattca
     6541 cgtcaatgga tccgccttcc aaaacgacgc gatgcagccc cgctgccgtc tcgatggtgg
     6601 gctcccaccg tggtaaaccg agcaaattg
//
LOCUS       DASZYN010000753         2333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_745195, whole genome shotgun sequence.
ACCESSION   DASZYN010000753 DASZYN010000000
VERSION     DASZYN010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745195"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VHE33_19280"
     CDS             complement(<1..243)
                     /locus_tag="VHE33_19280"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99651.1"
                     /translation="MKVVLAEKVSPATLAVFQSEPGWQVLTHDQVKNLPEALADADAL
                     VVRSAVQVDDALMAAGPKIRVIGRAGVGVDNIDAEAA"
     gene            552..1079
                     /locus_tag="VHE33_19285"
     CDS             552..1079
                     /locus_tag="VHE33_19285"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HVT99652.1"
                     /translation="MKALDVMSEYRRRRHDEAGFTLMELLIVISIMLILMLIAIPNML
                     NLKATANETSAKQSLRAIQEAETQYATNYPANGFACSLAALGGNASSGPPTPQAAQLL
                     QGDLAGGQKAGYSFNITNCQKAPGSQQDNFVSYEATAVPQVVGKTGHNGYCLDMSGEI
                     KSDPSGGTNCTIAIQ"
     gene            1260..1955
                     /gene="lolA"
                     /locus_tag="VHE33_19290"
     CDS             1260..1955
                     /gene="lolA"
                     /locus_tag="VHE33_19290"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HVT99653.1"
                     /translation="MNRITGNLSRQWAECRWRFLLVAVLGIGLAGGLHAQPAAGVAQA
                     AVRVDRHYNALHSLEVHFVQTYTGMGMNRKESGVLLLKKPGKMRWTYTDPDGKLFILD
                     GKYGYFYSPGATEAQKVEEKKLDDLRSPLRFLLGHTELQKELDGLHGNCDASGTCELA
                     GVPKHLEQRIASLKITAAPDGTIRSMTIEETDGVTNRFEFSGEAGNVPAADAAFQFVP
                     PPGVHVVEGEPPV"
     gene            complement(2102..>2333)
                     /locus_tag="VHE33_19295"
     CDS             complement(2102..>2333)
                     /locus_tag="VHE33_19295"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HVT99654.1"
                     /translation="VCELAGVPKHLEQRIASLKITAGPDGTIRSMTIEETDGVTNRFE
                     FSGEAANVPAADAAFQFVPPPGVHVVEGEPPV"
BASE COUNT          459 a          608 c          836 g          430 t
ORIGIN      
        1 ggcggcttcc gcgtcgatgt tgtcgacgcc gacgccggcg cggccgatga cgcggatttt
       61 ggggccggca gccatgaggg cgtcgtcgac ctggacggcg gagcggacga cgagggcgtc
      121 ggcatcggcg agggcttcag gcagattttt gacctggtcg tgggtcagga cttgccatcc
      181 gggttcggat tggaagacgg cgagggtggc cggagagact ttttcggcga gaacgacttt
      241 cacgatagct ccttactgaa cagattaatt gagggagagg tcgcgagcgc aggtcacatt
      301 gaagccagag ggcgggaaca ggcgagttta gtcgccaggg cgtgagcgga aaagaggttt
      361 tgtgcgtccg ttttgattgc ggagataccc tcggcgtgtg ccttgcgtgc gggatgccgt
      421 tagggaaagt gcactgaagt accgcgggcc acgcgaggcc gcggatcagc atttgcgata
      481 ggatggaagc ggattcggag cgcggacgcg gggcttttgc ggcgtggggg cgctcgaaag
      541 aagatgcagc tatgaaggct cttgacgtta tgagtgaata ccggcggcgc cgtcatgacg
      601 aggcgggttt cacgctgatg gaactgctga tcgtgatctc gatcatgctg atcctgatgc
      661 tgattgcgat tccgaacatg ctgaacctga aggcgacggc gaacgagacg tcggccaagc
      721 agtcgctgcg cgcgatccag gaagcggaga cgcagtatgc gacgaattat ccggcgaacg
      781 ggtttgcgtg ctcgctggca gcgctgggcg ggaatgcgtc gtcgggtccg ccaactccgc
      841 aggcggcaca actgctgcag ggcgatctgg cgggcgggca gaaggccggg tacagcttca
      901 acattacgaa ctgccagaag gctccgggca gccagcagga taactttgtg agctacgaag
      961 ccacggcggt gccgcaggtg gtgggcaaga cgggacacaa tgggtattgc ctggacatga
     1021 gcggggagat caagtccgat ccgtcgggcg gaacgaactg cacgatcgcg attcagtgag
     1081 gcgccgccgg caggcggctg cctcagccaa aaagcggttg gctgaagtcc ggtcagatgg
     1141 tgaagcggtt cgctgagggc cggttagctg aaaagcggca gggtatagcg tttaggggat
     1201 agcgtataac gtgcacgggc ccgcagggcc tggagcggct tccggaggat ggaagaggat
     1261 tgaatcggat aactggaaat ctgagccgcc agtgggccga gtgccgctgg cggtttttgc
     1321 tggttgctgt tctggggatc ggattggcgg gtggcctgca cgcgcagccg gcggccggtg
     1381 ttgcgcaggc cgcggtccgg gtggatcggc actacaacgc gctgcattcg ctcgaggtcc
     1441 actttgtgca gacttacacg ggcatgggga tgaaccggaa ggaatcgggt gtgctgctgc
     1501 tgaagaagcc ggggaagatg cggtggacgt acaccgatcc ggatggaaag ctgttcatcc
     1561 tggacggcaa gtatgggtac ttctactcgc cgggcgccac ggaagcgcag aaggtggagg
     1621 agaagaagct ggacgatctg cgctcgccgc tgcggtttct gctggggcat acggagttgc
     1681 agaaagagct ggatgggctg cacgggaact gcgatgcaag cggtacctgc gaattggccg
     1741 gggtgccgaa gcatctggag cagcggattg cgtcgctgaa gatcacggct gcgcccgatg
     1801 ggacgattcg atcgatgacg attgaagaga ccgatggagt gacgaaccgg ttcgagttca
     1861 gcggggaagc ggggaatgtg cccgcggcgg atgcggcgtt tcagtttgtg ccgccgccgg
     1921 gcgtgcatgt ggtggagggg gaaccgccag tgtgagcagg cagctggtgc ttttagcgaa
     1981 gagcgtttag ccagaagctt ttcgcgagaa gcttttcgca aggagcgttt agccagaagc
     2041 ttttcgcgaa ccgctcttta gctaaccgct ccaccagcga accgcctcac cagctgcctt
     2101 ctcacaccgg cggctccccc tcgaccacat gcacccccgg aggcggcaca aactggaacg
     2161 ctgcatccgc agccggcacg ttcgccgctt ctccactgaa ctcgaagcga ttcgtcaccc
     2221 cgtcggtctc ctcaatcgtc atcgatcgaa tcgtcccgtc aggacccgcc gtaatcttca
     2281 gcgatgcaat ccgctgctcc agatgcttcg gcacacccgc cagttcgcac acg
//
LOCUS       DASZYN010000754         9859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_305254, whole genome shotgun sequence.
ACCESSION   DASZYN010000754 DASZYN010000000
VERSION     DASZYN010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9859
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305254"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1601)
                     /locus_tag="VHE33_19300"
     CDS             complement(<1..1601)
                     /locus_tag="VHE33_19300"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99655.1"
                     /translation="MLKSVRPWAILAALAICPALTFAAPADKSDKKSDTKAADKSADK
                     TADKPAEKTAEKPPQEVTTEGTLDAGGQHILYNAIAGTITVGSSTGEDSQLGPDGKPL
                     PDTDLAAAVAAAKDPSQAPPLARMFYVAYFKRDAAADQRPITFIYNGGPGSSTVWLHM
                     GSFGPKHVVTEMDTHLPGAPYKLADNANSLLDVSDLVFIDMPGTGFGRLTGKDSGKAF
                     WGIDEDGHAFGRFIKRFLSKYNRWNSPKYIFGESYGTTRSAVLSNILENEETIDLNGV
                     ILLSQIFNFSTDIDGAHQNPGIDLTYALALPTYAATARYHKKLPQEHPDLQAFMKEVE
                     DFAMGPYTAALAKGTDLSQSEKQTIAQKLHDYTGLPVDYLMKGDLRIAGGQFEKTLQD
                     DTGDSTGRLDTRFTGPSLDPLSESVEYDPQSSAISSAYVALFNQYVRSTLKYGEGQTY
                     LPEADFSGGFQWDFKRQGFPLMNVSNDLATAMKTNPKLKVLVNGGYYDLATPFYAAQY
                     EDKHLPISSKLAENISYDWYESGHMV"
     gene            complement(1738..2712)
                     /locus_tag="VHE33_19305"
     CDS             complement(1738..2712)
                     /locus_tag="VHE33_19305"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HVT99656.1"
                     /translation="MTKSTKSPILFSMPDATLRAHAAAFRRFNRLYTRFIGTVTDRFL
                     RTPYSLAEGRVLYELATRDKPRAKDIAEALGMDAGYLSRVLTKHERAGLLKRKTSKTD
                     SRAADLLLTPKGRAVFRVLNQRSEKQARDVLTRMPAPTRTRFIEAIATMEHAILRTDA
                     EPPAFTLRSHRPGDMGVIVALEGKGYVDQFGWDSTFEALVARIVADFLDHFDPQKERC
                     WVAEIDGEHVGHIFLVRHPDQPGTAKLRLLYVDPKARGLGLGHTLVTECVRFAREAGY
                     QRITLWTQSILTAAHRLYQQAGFQLVREEAHHSFGKDLLGQTWELDLK"
     gene            2877..3482
                     /locus_tag="VHE33_19310"
     CDS             2877..3482
                     /locus_tag="VHE33_19310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HVT99657.1"
                     /translation="MREGKLLMIVGDYAEDYETMVPFQALQMVGHTVHAVCPGKKAGQ
                     QIRTAIHDFEGDQTYSEKRGHNFTLNATFAEVNPADYDGLVLPGGRAPEYLRLDETVL
                     ALIRSFIEEHKPIAAICHAAQLLAAAGVLQGRKVSCYPACAPEVHLAGGTYAAVEMTS
                     AVVDRNLVTAPAWPAHPAWLAKFNNLLQEHLAEKTEAVGAR"
     gene            3754..4761
                     /locus_tag="VHE33_19315"
     CDS             3754..4761
                     /locus_tag="VHE33_19315"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HVT99658.1"
                     /translation="MRKHLEITPNLEPLFGTRDENLRLMEDALRVRIDLVSDAVTVEG
                     PEESIARVERIFHDYEALRRQGILLQNGELNGMLRLVTADPSVSLRSLAESGKQRSAG
                     TKRMVQPRSINQRLYVEAIEREDMVFGIGPAGTGKTYLAVAMAASALLAKKISRIVLV
                     RPAVEAGERLGFLPGTLQEKVDPYLRPLYDALYDLLEPERVDKMLERNVIEVAPLAFM
                     RGRTLNDAFIIMDEAQNTTSEQMKMFLTRMGNNAKAVITGDITQIDLPNPRKSGLVEA
                     MNILDGVDGIRFIRFEDGDVVRHHLVQRIIRAYEGFGRAQQELPLAIDGEDEVRVSQP
                     Q"
     gene            4951..5451
                     /gene="ybeY"
                     /locus_tag="VHE33_19320"
     CDS             4951..5451
                     /gene="ybeY"
                     /locus_tag="VHE33_19320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HVT99659.1"
                     /translation="MILIEPTIQTQYSRALRQRALSAFLREAAPAAGLRGEVSVLLTG
                     DRQIRRLNREFRGKDKATDVLSFPAAEPLNGHAGLAGDLAVSVETAVRQAKEFGHPLA
                     TELQILVLHGLLHLSGYDHEADEGQMARKELSLRRRFGLTAGLIERSAPPAVRGAVST
                     RQRRRP"
     gene            5448..6728
                     /locus_tag="VHE33_19325"
     CDS             5448..6728
                     /locus_tag="VHE33_19325"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HVT99660.1"
                     /translation="MIVTAAVLIVLLLTVLTLVSYVDRLYSEMGKFLAREFQENIDLW
                     EQRVEPRLGMNRDRIALSAAILMHLSLAFLTLVFGMLLFARARAGDHATFAEIAQVVL
                     GIVLVIVVFNRLLPYAFFARTRGAWIVRWRWFLRVLLWMVLPITLFVQFLVSIAALAE
                     ESANANGDTTAAEAVEALIEAGEEEGIIEESDRDLVRSAVEFGDKVVREVMTPRPAMF
                     AVPGTITLEQFLELIRAQGFSRVPVYRETLDNVTGIAFTHDLLQISDAEARSKTVASI
                     QRPAAFVPETKKVNELLREMQREKQHMRIVIDEYGGVAGLVTIEDLLEELVGAIRDEH
                     DDEESAAVIREPGGTWVVPGNLEIARVEEILGGVELPEDVEATSVGGLVSETAGRIPQ
                     SGEVVEGYGLRFEILASTDRKVNRLRVSRLARAS"
     gene            6756..7775
                     /gene="era"
                     /locus_tag="VHE33_19330"
     CDS             6756..7775
                     /gene="era"
                     /locus_tag="VHE33_19330"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HVT99661.1"
                     /translation="MKRRSGFVSIIGRPNAGKSTLLNALIGEKVAIVTAKPQTTRTRI
                     HGIVEVPERKGKHPATQIVFVDTPGIHRPASQLDRSMMHEIHEALMTRDLVLLVVDAI
                     RKQPHRVEDGILKLDRASEEALAFEMIRELDCPVFLVVTKIDLVSKEALLPLIEGLSG
                     LHKFAEVIPVSAKKGDGLKLLQHKIAEYLPEGDRWFPKEQFTDQPERFLAAELIRERI
                     LMETGEEVPYASAVIVERFEEPEPQPEKKRRPEGKPPLTRISAAIYCERDGQKAILIG
                     KGGSKLKEIGAGARKEIEALLGTRVYLELRVLVREHWRDSRVFVDSLDWRKQLEELSE
                     RRGRQ"
     gene            complement(7813..8571)
                     /locus_tag="VHE33_19335"
     CDS             complement(7813..8571)
                     /locus_tag="VHE33_19335"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HVT99662.1"
                     /translation="MPGLLRASFRLLILLASLVEVAMRYFILRLKHGSPPELWQRAHW
                     LHAACQLILRRLGLGLQWRGTRPSHGLICSNHLSYLDILVYAAATPCVFVSKQEVGRW
                     PAFGFFARCGGTIFLDRESRLSAEKAANQMASVLQANVPILLFPEGTSTDGVEVLRFH
                     PALLEPAIQLDQEITAAAIAYRAHGVEERDLCWFGDDPFLPHLLRTMARRSIAVEVEF
                     HPDRRTYPDRKTAALELREQVEAMRKRMKREATP"
     gene            complement(8535..9428)
                     /locus_tag="VHE33_19340"
     CDS             complement(8535..9428)
                     /locus_tag="VHE33_19340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HVT99663.1"
                     /translation="MHTHKFSVLPRRIAFGVGAPVEIQTGGFARSAEAFAPQTPHITL
                     ELGKYEIRLAESLAEREAACRLRFKVFNIELGEGLESSCQTGLDQDQFDLFCDHLIVE
                     DRMRREVVGTYRMQSGNSALQKMGYYSEQEFDFSPYEAIRAQVLELGRAAIHREHRTS
                     EVLTLLWRGITQYARFYRLRYLIGCSSLNSRNPGEGWAILRQLEPFLAPETLRTRPTF
                     SYELPRTTKEVVADVKVPKLLKTYIAVGARICGAPAWDREFGTIDFLTLLDLAHLSPA
                     ARSRFMPESDCLASSEPVSAY"
     gene            9645..>9859
                     /locus_tag="VHE33_19345"
     CDS             9645..>9859
                     /locus_tag="VHE33_19345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99664.1"
                     /translation="MTREELVAAVDEEISRLEKVRALLLSSGGSRISSSSFGNKPRKK
                     RVLSADARARIAAAQRRRWAKQKAAQN"
BASE COUNT         1926 a         2573 c         3464 g         1896 t
ORIGIN      
        1 accatgtggc cggactcgta ccagtcgtag ctgatgttct cggccagctt gctcgagatc
       61 ggcaggtgct tgtcctcgta ctgagccgcg tagaagggcg tggccagatc gtagtagccg
      121 ccgttcacca gcaccttcag cttcggattg gtcttcatcg cggttgcgag gtcgttgctg
      181 acgttcatca gcgggaagcc ctggcgcttg aagtcccact ggaagccgcc gctgaagtcc
      241 gcttcgggca gataggtctg gccctcgccg tacttcagcg tgctgcgaac gtactggttg
      301 aagagcgcga cgtaggccga ggagatggcc gaactctgcg ggtcgtattc gacactttcg
      361 ctcaagggat cgagcgaggg cccggtgaag cgcgtgtcga gacggccggt cgagtcgccg
      421 gtgtcgtcct ggagggtctt ctcgaactga ccgcccgcaa tgcgcaggtc gcccttcatc
      481 aggtagtcga ccggcaggcc ggtatagtca tgcagctttt gggcgatggt ttgtttctcg
      541 ctctggctca ggtcggttcc tttggccagc gccgccgtat agggccccat agcgaagtcc
      601 tccacctcct tcatgaaggc ctgcaggtcg ggatgctcct ggggcagctt cttgtggtag
      661 cgggccgtgg ctgcataggt aggcagggcg agcgcatagg tgagatcgat gcctgggttc
      721 tgatgggccc catcgatatc ggtgctgaag ttgaagatct gcgagagcag gatgacgccg
      781 ttgaggtcga tggtctcctc attttcgagg atgttcgaca agacggccga gcgcgtggtg
      841 ccgtagcttt cgccgaagat gtatttcggc gagttccagc ggttgtattt ggagaggaag
      901 cgcttgatga agcggccgaa ggcatggccg tcctcgtcga tgccccagaa ggctttgccg
      961 gagtccttgc cggtgaggcg gccgaaaccg gtgccgggca tatcgatgaa gaccaggtcg
     1021 ctgacatcga gcagcgagtt cgcattgtcg gccagtttgt agggagcgcc gggcagatgc
     1081 gtgtccatct cggtgacgac atgttttggt ccgaaggagc ccatgtgcag ccagaccgtc
     1141 gagctgcccg ggccgccgtt gtagatgaag gtgatggggc gctggtcagc ggccgcatcg
     1201 cgcttgaagt aggcgacgta gaacatgcgc gccagcggcg gagcctggct gggatccttg
     1261 gcggcggcaa ccgcggcggc gaggtcggta tcgggtagcg gcttgccgtc gggcccgagc
     1321 tgcgaatctt cgccggtgct ggagccgacc gtgatggtgc cggcaatggc gttgtagagg
     1381 atgtgctggc cgccggcatc gagtgtgccc tcggtggtga cttcctgcgg tggtttctca
     1441 gccgtttttt cggccggctt gtcggcggtt ttgtcggcag acttgtcggc ggctttcgtg
     1501 tcggacttct tatcggattt gtcggctggg gctgcgaagg tgagggcggg acagatcgcg
     1561 agggcggcga gaatcgccca cgggcgtaca gatttcaaca tgcactggct cctgagtgaa
     1621 acaagaatga gaactgaaga tcgatgagga ccgaggatag ggggaacgca gattatagag
     1681 cgcggcgagt gaaagcggtc aagccatgtg accggagggc tctgagcggg gctggtgtta
     1741 tttcaggtcc agctcccagg tttggccgag cagatccttg ccgaagctgt gatgggcttc
     1801 ctcgcggacg agctgaaatc cggcctgctg gtagaggcgg tgcgcggcgg tgaggatact
     1861 ttgcgtccag agcgtgatgc gctggtagcc ggcctcgcgg gcgaagcgaa cgcactcggt
     1921 gaccagggtg tggccgaggc ccagtccgcg cgctttcgga tcgacgtaga gaaggcggag
     1981 tttggcggta ccaggctggt cgggatggcg cacgaggaag atgtgaccga cgtgttcgcc
     2041 gtcgatctcg gcgacccagc aacgttcttt ctgcggatcg aagtggtcga ggaagtcggc
     2101 gacgatgcgg gcgacgagag cttcgaaggt ggaatcccag ccgaactggt cgacgtagcc
     2161 tttgccctcg agagcgacga tcacgcccat gtcgccggga cggtgggagc ggagggtgaa
     2221 ggcgggcggc tccgcgtcgg tgcggagaat ggcgtgctcc atggtggcaa tggcttcgat
     2281 gaagcgggtg cgcgtgggcg caggcattcg ggtgagaacg tcacgggcct gcttctccga
     2341 gcgctggttg agcacgcgaa agacggcgcg gcctttgggg gtcaggagaa gatccgcagc
     2401 gcggctgtcg gtctttgagg ttttgcgctt cagcaatccg gctcgttcgt gtttggtgag
     2461 gacgcggctg agatagccgg catccatgcc gagggcttcg gcaatgtctt tggcgcgagg
     2521 cttgtcgcgg gtcgccagct cgtagaggac gcgcccttcg gcgaggctgt agggggtgcg
     2581 caggaagcgg tcggtgacgg ttccgatgaa gcgggtgtag aggcggttga agcgccggaa
     2641 ggcagcagca tgggcgcgga gggtggcatc cggcatggaa aagaggatgg gtgatttagt
     2701 tgactttgtc aagtatcacg cctgcctgac cggaagacgt tttgcggagg agaaaagcaa
     2761 ccgcaggttc ttccgccgcg cgatgcgcga cgtcagaaag acaagcctaa ggtgtgggac
     2821 gacaaagcgg tgacagagcg ctatgatgcc ccgtcagacg aattgtggag gagagcatgc
     2881 gcgagggaaa gcttctgatg atcgtcggcg actacgccga ggactacgag accatggtgc
     2941 cgttccaggc gctgcagatg gtggggcata cggtgcatgc ggtgtgtccg gggaagaagg
     3001 cggggcagca gattcggacc gcgattcatg acttcgaggg cgaccagacc tattcggaga
     3061 agcgcggaca caacttcacg ctgaatgcga cgtttgccga ggtgaatccg gcggactacg
     3121 acgggctggt gctgccgggt gggcgggcgc cggaatatct ccgcctggac gagacggtgc
     3181 tggcgctgat tcgctctttc atcgaggagc acaaaccgat tgcggccatc tgccatgcgg
     3241 cgcaattgct ggcggcggcg ggggttctgc agggccggaa ggtgagctgt tatccggcgt
     3301 gcgcaccgga ggtgcatctg gccggcggaa cctacgccgc ggtggagatg acctcggccg
     3361 ttgtggatcg caatctggtg acggcgccag cgtggccggc gcatccggcg tggctggcga
     3421 aattcaataa cctgctccag gagcatctgg cggagaagac ggaggcggtg ggggcccggt
     3481 aagtggttgg aaatgagcta atcggggctg taccacttcg gctcgaaatt ccggaaatat
     3541 tcaacaggga gtattgactg ggatgtaatc cgggactact ctgaccgcaa cttcgacctt
     3601 gttgccgaat gccaatccgt tgcgccgtgc ctgcctgaag catctcatca gtgtggagag
     3661 attccggttg cggccttcgg cgatcctttc gaaaactcaa ttccagcccc gtggcccgcg
     3721 ccgagccacg tttggaggtc tttccctctt ctgatgagaa aacatctcga gatcactccc
     3781 aatcttgagc ccctgttcgg gacccgcgac gaaaatctga gactcatgga ggatgccctc
     3841 cgtgtccgta tcgatctggt atccgatgcg gtgacggtgg aggggcccga ggaatccatt
     3901 gcgcgtgtgg agcggatttt tcacgactac gaagcgctcc gccgccaggg aatcctgctg
     3961 cagaatggcg agctgaacgg gatgctgcga ctggtgacgg cggatccgtc ggtatcgctg
     4021 cgcagtctgg ccgagagcgg gaaacagcga tccgcgggca ccaaacgcat ggtgcagccg
     4081 cgctcgatca accagcggct ctatgttgaa gccatcgagc gggaagatat ggtgtttggc
     4141 attggcccgg cgggcacggg aaaaacctat ctggcggtgg ccatggcggc ttcggcgctg
     4201 ctggcgaaga agatcagccg cattgtgctg gtgcggccgg cggtggaagc tggcgagcgg
     4261 ctgggattcc tgccaggaac gctgcaggag aaggtggatc cctatctgcg gccgctctac
     4321 gacgcccttt acgatctgct ggagccggag cgcgtggaca agatgctgga gcgcaacgtg
     4381 atcgaggtgg caccgctggc gtttatgcgc gggcggaccc tgaacgatgc attcatcatc
     4441 atggacgagg cgcagaacac gaccagcgag cagatgaaga tgttcctgac ccgcatgggc
     4501 aacaatgcca aggcggtcat caccggggac atcacgcaga tcgacctgcc caacccgcgg
     4561 aagtcgggtc tggtggaggc gatgaatatt ctggacgggg tggacggaat ccggttcatc
     4621 cggttcgagg acggagacgt cgtccggcac catctggttc agcggattat ccgcgcctat
     4681 gaaggctttg ggcgcgcaca acaggagctg ccgctggcca tcgacggcga ggacgaggtt
     4741 cgggtctcgc agccccaata gtctttcgca gcccgccgtg ttgcgcctaa aagccgcaaa
     4801 atgagggatt aggcggccgg caatcatggg gaggctacgg cagaccgcgt aaatgaattc
     4861 attcagaggt accatcgaag tgggagggtt taaccctccc accgttgttt tgtgcgccgg
     4921 attgctccag gggccgggcc gcaccggacc atgattctga tcgagcccac gatacaaacc
     4981 caatacagcc gcgcgttgcg gcagcgggcc ctcagcgcat ttttgcgtga ggccgcgccg
     5041 gctgccgggc tgcgcggcga ggtatcggtt ctgctgacag gggatcgaca aattcgcagg
     5101 ctcaaccggg agtttcgggg caaggacaag gcgacggatg tgttgtcgtt cccggcagct
     5161 gaaccgttga atgggcacgc aggcttagcc ggggatctgg ccgtatcggt cgagacggcc
     5221 gtacggcagg caaaggagtt cgggcacccc ctggcgacag aactgcaaat cctggtgttg
     5281 cacgggctgc tgcatctgag cggctacgac cacgaggccg atgaggggca gatggcgcgc
     5341 aaggagctta gcctgcgccg tcggtttggg ctgacggcag ggctgatcga gcggtcggct
     5401 ccgccagcgg tcagaggcgc agtttcgaca cggcaacgga ggcggccatg attgtgacgg
     5461 ctgcggtgct gattgtcctg ctgctgacgg tgctgacgct ggtgtcgtat gtcgaccgcc
     5521 tgtactcgga gatgggcaaa ttccttgcgc gcgaattcca ggaaaacatc gacctgtggg
     5581 agcagcgggt ggaaccgcgg ctaggcatga accgggaccg gatcgcgctg tcggcggcga
     5641 tcctgatgca tctctccctt gcgtttctga cgctggtctt cggaatgctg ctcttcgcac
     5701 gggcgcgggc cggggatcac gccacctttg cggagatcgc gcaggtggtg ctcgggatcg
     5761 tgctggtgat tgtggtgttc aaccggctgt tgccctatgc cttctttgcg cgcacccggg
     5821 gagcgtggat tgtccggtgg cgatggtttc tgcgggtgct gctgtggatg gtgctgccga
     5881 tcacgctgtt cgtgcagttt ctggtttcca ttgcggcgct cgccgaggag tcagccaatg
     5941 cgaacggaga cacgaccgcg gcggaagccg tggaagcgct gattgaagcc ggcgaggaag
     6001 aggggatcat cgaggagagc gatcgcgacc tggtgcggtc ggcggtcgag tttggcgaca
     6061 aggtggtacg ggaggtgatg acgccgcggc cggcgatgtt tgccgtgccg gggacgatca
     6121 ccctggagca gtttctggag ctgatccggg cgcagggatt ttcgcgtgtg ccggtctacc
     6181 gggaaaccct ggacaacgtg acgggcattg cttttacaca cgatctgttg cagatttccg
     6241 acgccgaagc gcggtcgaag accgtggcat cgatccagag gccggcggcg tttgttcccg
     6301 agacgaagaa ggtgaacgag ctgctgcgcg agatgcagcg ggaaaagcag catatgcgca
     6361 tcgtgatcga cgagtacggc ggcgtggcgg ggctggtgac gatcgaggat ttgctcgagg
     6421 aactggtggg agcgattcgc gacgaacacg acgatgagga aagcgccgcg gtgatccggg
     6481 agccgggggg gacctgggtg gtgccgggca atctggagat cgcgcgcgtg gaggagattc
     6541 tgggtggcgt ggagctgccg gaagatgtgg aggctaccag tgtgggcgga ctggtgagcg
     6601 agacggcagg acggattccg cagtcgggcg aggtggtgga gggttacggg ctgcgctttg
     6661 agattctggc ctcgaccgac cgcaaggtga atcgtctgcg ggtttccaga ctggcaaggg
     6721 ccagctaagc tgaatcgaac agacaggaag aacgcatgaa gaggcggtcg gggtttgttt
     6781 ccatcatcgg gcggccgaac gcgggcaagt cgacgctgct gaacgcgctg atcggcgaaa
     6841 aagtcgccat cgtcacggcg aaaccgcaga cgacgcgcac gcggatccat ggcatcgtgg
     6901 aggtgccgga gcggaaagga aagcatccgg cgacgcagat tgtgtttgtg gatacgccgg
     6961 ggattcaccg gcccgcctcg cagctggacc gcagcatgat gcatgagatt cacgaggcgc
     7021 tgatgacgcg cgacctggtg ttgctggtgg tggatgcgat ccgcaagcag cctcatcgag
     7081 tggaggacgg aattctgaag ctcgatcgcg ccagcgaaga agctttggcg ttcgaaatga
     7141 tccgagaact ggactgcccg gtctttctgg tcgtcacgaa gatcgacctg gtttcgaaag
     7201 aggccctgct tccgctgatc gaagggctca gcgggctgca caaattcgcg gaggtgattc
     7261 cggtttcggc aaagaagggc gatgggctca agctgcttca gcacaagatt gccgaatatc
     7321 tgcccgaagg cgaccggtgg tttccgaagg agcagtttac ggatcagccg gagcggtttc
     7381 tggcggcgga actgattcgc gagcgcattc tgatggaaac gggcgaagag gtgccgtatg
     7441 catcggctgt gatcgttgag cgcttcgaag aacccgagcc tcagccggaa aagaagcgcc
     7501 gcccggaggg caaacctccg ttgacgcgga tctcggcggc gatctactgc gagcgtgacg
     7561 gacaaaaggc catcctgatc ggcaagggcg gcagtaagct gaaggaaatt ggcgcgggcg
     7621 cgcgaaagga aattgaggcg ctgttgggaa cgcgcgtcta cctggagctg cgtgtcctgg
     7681 tacgggaaca ctggcgcgac tcgcgggtat ttgtcgattc actggactgg cggaagcagc
     7741 tggaggaact ctccgagcgg cgaggcaggc agtaggcaag taggcagtag gcagtaattg
     7801 gaccgggctt tgctacggcg tcgcttctct cttcatccgt tttctcatgg cttcgacctg
     7861 ctcgcggagt tcgagtgcgg cggttttgcg atcgggataa gttctgcgat ccgggtgaaa
     7921 ctcgacttcg accgcgatgc tgcggcgcgc catggttcgc agcaggtggg gtaagaaggg
     7981 atcgtccccg aaccagcaga gatcgcgttc ttcgacaccg tgtgcccgat aggcgatggc
     8041 cgctgcggtg atttcctggt cgagctgaat ggcaggttcc agcagcgcag ggtggaagcg
     8101 caggacttcg acgccgtcgg tgctggtgcc ttcgggaaac agcaggatgg gcacattggc
     8161 ctggaggacg gaagccattt gattcgctgc cttctcggcg ctcaaacggc tctcacggtc
     8221 gaggaagatg gttcctccgc aacgcgcgaa gaagccgaag gcgggccagc ggccaacttc
     8281 ctgcttggag acaaagacac agggcgttgc tgcggcatag acgaggatgt cgagatagct
     8341 aaggtgattg gagcagatca aaccgtgaga cggtcgtgta ccgcgccact gcagccccaa
     8401 tcccaggcgc cggaggatga gctggcatgc tgcgtggagc cagtgcgcac gctgccacaa
     8461 ttccgggggc gaaccatgct tcagccgcag aatgaaatag cgcatggcca cttcgaccag
     8521 gctggccagg agaatcagta agcggaaact ggctcggagg aggccaggca atccgactcc
     8581 ggcatgaagc ggctgcgcgc ggccggcgag aggtgagcca gatcgagaag agtcagaaag
     8641 tcgatcgttc cgaattcgcg atcccatgcc ggtgcgccgc agattctggc tcccactgca
     8701 atatacgtct tcaggagttt ggggaccttc acgtcagcaa cgacttcttt ggttgttcgc
     8761 ggcaactcat aactgaaagt gggcctggtg cgcaaagtct ccggagccag aaatggttcc
     8821 agttgacgaa gaatggccca accctcgccg ggattacggg aattcaacga ggagcaacca
     8881 atgaggtagc ggagacgata gaagcgcgcg tactgggtga tgccgcgcca aagcagggtg
     8941 agtacttcgc tggtgcgatg ctcccgatgg atggcagcgc ggcccagttc gaggacctgg
     9001 gcccgaatcg cttcataggg cgagaagtcg aattcctgtt cactgtagta acccattttc
     9061 tgcagagcgg aattaccaga ctgcatccta taggtaccca caacttcacg gcgcattcga
     9121 tcttcaacaa tgaggtggtc gcagaagaga tcgaactggt cctgatccag accggtctgg
     9181 caggaagatt cgagtccttc acctagttcg atattgaaaa ctttgaaacg cagccgacaa
     9241 gcggcctcgc gttcggccaa actttcggcg aggcgaattt cgtatttgcc cagttccagc
     9301 gtgatgtggg gcgtctgcgg cgcgaaggct tccgcggaac gagcgaagcc gccggtctgg
     9361 atctccacag gggcgccgac gccaaaggca atgcgacgtg gaagtacaga gaatttgtgg
     9421 gtgtgcacgc ctctatgctg cggagggagg aagaaaagga gcttacagcg gagagaaaag
     9481 catgcaaatt ctaagttaat gtgcaatttc agcacatcga cgatggcctt cgttccaggg
     9541 cgggcgagcg gtttttttgt aacaaacctc tcaatttgtt atggtaggcg gtgttgtatc
     9601 cgttccctgg ataacttacc tgtaccacac gagagaaaaa aataatgaca cgcgaagaac
     9661 tagttgctgc agtcgatgaa gaaatcagcc gcctcgagaa agttcgtgcc ttgctcctca
     9721 gcagcggagg ctcccgtatc tcgtcttctt ccttcgggaa taagccccgg aaaaagcgtg
     9781 ttctaagtgc ggacgcgcgc gcccgcattg ccgcagcgca gagaaggcgc tgggcaaagc
     9841 aaaaggcggc acagaacaa
//
LOCUS       DASZYN010000755         3746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_110676, whole genome shotgun sequence.
ACCESSION   DASZYN010000755 DASZYN010000000
VERSION     DASZYN010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110676"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2391
                     /locus_tag="VHE33_19350"
     CDS             <1..2391
                     /locus_tag="VHE33_19350"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HVT99665.1"
                     /translation="LGWFFILLHFGFLLLNPADVWGQKLVVTVAESALLLCGAAFALS
                     SGLIRETGWRYAVPAVLLSIPPVACVSVVTIWHAPVGLLVGPGVVAQAAYGAVALRFG
                     RGRKSVVGRGRIARRWAAAILASGTAWMLALLWRGHADLSFYPLLSEIFLLNAVLYWE
                     DFRRTTAGVLVSVSGLAAWGGVFPCAVLMAAFLPRIQVIGEMWNVPKYFVAFGMILTL
                     LEDQKRAADARSEEYRVLFENNPHPMWIFDTETLRFLAVNDAAVARYGYTVEEFRSMT
                     LRDIRPPEEVAAMEQKMSQIAGDETVVISGPWTHILKDGTSIQVEVSAHTIQFEGRPA
                     RFSLVQDVTERQQLHHRLVHQAHHDTLTGLPNRLLLLDRMEQALASAARRGKKAAVIC
                     LDIDRFKQINDTYGHAVGDLCLKQVAERLKSRLRGDDTVARSGGEEFTVIVGDLVAAE
                     DAARVAEDLLSAVRKPLAAEEYPIDVTASFGIAIYPDHGLDASSLWRSADAAMYRVKR
                     SGGNDYVLVSHEISQSTTEANELELFMRRALKEGGLELFYQPEYLADGRICGLEALLR
                     VQHPKFGVVHPDRFIPIAEESGLIVPMGNWVLREVCRQSEEWRRLGLDVPRIAINVSP
                     LQFMRVDFSRQVRQAVMETGIDPDTLEIEMTETTVMRNLDELARQMRDLADLGVHFSV
                     DDFGTGYSSLQHLHQLPIERLKIDRSFIEQIGEEASGTWALVRAILSLAHSLGLQVVA
                     EGVEREEQAEALASMRCDMMQGYLFAPPRAAQDIPALIRLGHAPMSWRPIPVRAAV"
     gene            2736..>3746
                     /locus_tag="VHE33_19355"
     CDS             2736..>3746
                     /locus_tag="VHE33_19355"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HVT99666.1"
                     /translation="MQQLMRGKQISAAPESKDVSLTDLWLVIRKRRVLLAVMAIVVGL
                     LAAGRGFLHGKWYTATGEVRIQPGSGSEFKQTGSLLLGSYGTLDVTIESDIRILQSRK
                     LLTAVAEKLSLQDNPEFMGRGISVKPGFLGGKNAIPEHRSLDDPQLQNAVVGNLRSHL
                     TAVRVPRTQMITISYTSPSPKLSSEIVNALQNEFIENNFTTHYNTTQEVSKWLTGRMD
                     DLRSVVQDSQNKMVDLQRKLGVMALDPEHSLMVQDIGALQKNLADATEQRVLAEARYR
                     ILKSLPPDQIQEGGTAGLPSIAGVSGAGGSEGLLANLRTQRAGIEAELAHLQPVYGPN
                     YPQ"
BASE COUNT          721 a          986 c         1308 g          731 t
ORIGIN      
        1 ctgggatggt tcttcatcct gctgcatttt ggatttctgc tgctgaaccc ggcggatgtg
       61 tggggacaga agctggtggt cacggtggcc gaaagcgcgc tgctgctgtg cggcgcagcg
      121 tttgcgctgt cgtccggcct gatccgggaa acaggttggc gttatgcggt gccggccgta
      181 cttcttagta ttccgccggt cgcctgcgtg tcggtggtga cgatttggca tgcccccgtg
      241 ggtttgctgg tggggcctgg agtcgtggcc caggctgcct atggggcggt ggctctgcgt
      301 ttcggacggg gacgcaagag tgtggtgggc cgtgggcgga tcgcacgacg gtgggcagca
      361 gccatcctgg cgagcggaac agcatggatg ctcgcgctgc tgtggcgtgg tcatgcggac
      421 ctgagctttt atccattgct gtcggagatt tttcttctga acgcggtgct gtactgggag
      481 gatttccggc ggacgacggc gggggttctg gtgtcggtgt ccggcctggc ggcgtggggc
      541 ggtgttttcc cgtgtgcggt cctgatggcg gcgtttcttc cccgcattca agtgatcgga
      601 gagatgtgga atgtgccgaa gtatttcgtc gccttcggca tgatcctcac tctgctggaa
      661 gatcagaagc gggcggcgga tgcacgcagc gaggagtatc gcgttctttt tgagaacaat
      721 ccgcacccga tgtggatctt cgacaccgag acgctgcgtt ttctggcggt gaacgacgcg
      781 gccgtggcac gctacggcta caccgtggag gaattcagga gcatgacgct gcgggacatt
      841 cgtccgccgg aagaggtggc cgcaatggaa cagaagatgt cccagatcgc gggcgacgaa
      901 acggtagtca tctccgggcc gtggacgcac attctgaagg atgggacaag cattcaggtt
      961 gaggtatcgg cccatacgat tcagttcgaa ggccggccgg cgcgattctc tctggtgcag
     1021 gatgtgacgg agcggcagca gctgcaccat cggctggtgc atcaggcgca tcatgacacg
     1081 ctaaccgggc ttccgaaccg cctgctgctg ctggaccgca tggagcaggc gctggcatcg
     1141 gcagcgcgcc ggggaaagaa ggccgccgtg atctgcctgg acatcgaccg tttcaagcag
     1201 atcaacgaca cctatggcca cgcggtggga gatctttgcc tgaaacaagt cgcggagcgt
     1261 ttaaagagcc ggctgcgcgg agacgacacc gtggcccgat ccggcggcga agagttcacc
     1321 gtcattgtgg gcgatctggt tgccgccgag gatgccgcgc gcgtggcgga ggacctgctg
     1381 tccgctgtgc gtaagccact ggccgcggag gagtatccaa tcgacgtgac ggcaagtttt
     1441 gggatcgcca tttatccgga tcacggcctg gacgcatcgt cgttatggag gagcgcggac
     1501 gccgccatgt accgggtgaa gcggtccggg ggaaatgatt acgtactggt atcgcacgag
     1561 atcagccagt cgacaacgga ggcgaacgag ctggaactct tcatgcgccg ggcgctgaaa
     1621 gagggcggtc tggagctgtt ttaccagccg gaatacctgg ccgacgggag gatctgcggg
     1681 ctggaggcac tgctgcgcgt ccagcatccg aagttcggcg tggtgcaccc cgaccggttt
     1741 attcccatcg cggaggagag cggcctgatt gtgccgatgg ggaactgggt gctgcgggag
     1801 gtttgccggc agagtgagga atggcgccgg ctggggctgg acgttccgcg catcgcgatc
     1861 aatgtttcgc cgctgcaatt catgcgcgtg gacttctcgc gccaggtgcg tcaggcggtg
     1921 atggaaaccg ggatcgatcc cgataccctg gagatcgaga tgaccgagac gacggtgatg
     1981 cggaatctcg acgaactggc ccggcagatg cgcgatctgg cggatctggg ggtgcatttt
     2041 tcggtggatg atttcggaac gggctattcc tcgctgcaac atctgcatca gctgccgatt
     2101 gagaggctga agatcgaccg atcgttcatc gaacaaatcg gagaggaggc aagcggcacg
     2161 tgggctctgg tgcgggcgat tctgtcgctg gcacacagcc tggggctgca ggtagtagcg
     2221 gaaggagtag aacgggagga acaggccgag gctctggcca gcatgcggtg cgacatgatg
     2281 cagggctatc tgtttgctcc gccgcgagcg gcccaggata tcccggcgct gatccgtctg
     2341 gggcatgcac cgatgagctg gaggcccatc cccgtgcggg ctgcggtctg agcatgggac
     2401 ggctcagcag gcggcttcga gcgcccgggg aaaggcgcgg gaaagacacg ccagaccccc
     2461 aaaaagcggg ggtgtcaaaa gaggacacgc cggggtactg tttgattgac cggtaaggaa
     2521 acccggctgt acactcgaac cacgcttggc ttaaaaagcg gtaacacttg ggcgaaatgc
     2581 atggcgcacc gccagccctg tttctgtgga ttgaccggaa gcaagcccta cttttttgtg
     2641 tgtggccgcg ctctggatcc aggggcggca acaattcaca atcagggtcg tcaatgcagg
     2701 gatgccggtc aggaatcggc acactgagag gttaagtgca acagttgatg cgcggcaagc
     2761 agatctccgc tgccccggag agcaaagatg tatcactcac cgacctgtgg ctggtcattc
     2821 gcaagcggcg tgtgcttctg gccgtgatgg cgattgtggt ggggctgctg gcggcgggcc
     2881 gtggcttcct tcatgggaaa tggtatacgg ccacaggcga agttcggatc cagccgggat
     2941 cgggatcgga gttcaagcag accggttctt tgcttctggg ctcctacggc acgctggacg
     3001 taaccatcga gagcgacatt cggattctgc agagcagaaa gctgctgact gccgtagcgg
     3061 agaagctgag cctgcaggac aatccggagt ttatgggacg cgggatttcg gtcaagcccg
     3121 gatttctggg agggaagaat gcgattcccg aacaccgcag tctcgacgat ccgcagttgc
     3181 aaaatgcggt ggtggggaac ctgcgcagcc atctgacggc cgtgcgcgtg ccgcgcaccc
     3241 agatgatcac gatttcctac acaagtccgt cgccaaagct ttcttcagaa attgtgaacg
     3301 cgttgcaaaa cgagttcatc gagaataact tcacgactca ctacaacacc acgcaggaag
     3361 tgagcaaatg gctcaccggg cggatggacg atctgcgctc ggtagtccag gattcgcaga
     3421 acaagatggt ggatctgcag cgcaagctgg gggtgatggc gctcgatccc gaacatagcc
     3481 tgatggtcca ggatattggg gcgctccaga agaatttagc cgacgcgacc gagcagcgcg
     3541 tgctggccga agcgcggtac cggatcctga agtccttgcc gccggatcag attcaggagg
     3601 gcggaacggc gggacttccg agcattgcag gagtaagcgg agcaggaggc tcggagggtt
     3661 tgctggcgaa tcttcgtacg cagcgggcgg gcatcgaggc cgaactcgcg cacctgcagc
     3721 cggtttatgg accgaattat ccgcag
//
LOCUS       DASZYN010000756         5250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_244349, whole genome shotgun sequence.
ACCESSION   DASZYN010000756 DASZYN010000000
VERSION     DASZYN010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244349"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /gene="queF"
                     /locus_tag="VHE33_19360"
     CDS             <1..346
                     /gene="queF"
                     /locus_tag="VHE33_19360"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HVT99667.1"
                     /translation="DFPAIETWRNQFPGYEIVIDDPEFTSVCPKTGLPDFGVITLRYM
                     PREQCLELKSWKEYLFSYRNLGIFQENVVNRILEDVVKACDPVWAVVKGDFRPRGGIS
                     TTIEARWPRPKQ"
     gene            complement(414..1127)
                     /locus_tag="VHE33_19365"
     CDS             complement(414..1127)
                     /locus_tag="VHE33_19365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99668.1"
                     /translation="MANTPLQVGTSLSGNWVFAPTSSGVVLNVGFMQGAYETVSAVAR
                     LNGSSCVSPSSDVLLTGSVGLDNKMMLISSPFSGTTLTLQGQVSGDGKGIASARWSFA
                     GGSCASLGAAAVTATNYSAINGTYTGTFVDDSNDSIPVSAVLEQTSQPDQNGQFSLSG
                     TASFPGNSCFVNQPSVTTSTVTGSSLAMTYSDPGSSTKLTAIGTFNAQASQLTITNWS
                     ISGGPCNGDSGSGSLAVQQ"
     gene            complement(1365..2108)
                     /locus_tag="VHE33_19370"
     CDS             complement(1365..2108)
                     /locus_tag="VHE33_19370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99669.1"
                     /translation="MVTVAAVLAAACQAQVSQPPGNMPGRHFPFPPRQGVSTPPAAAP
                     GPEVQGQPSIHTGAVTSAPNGPQPAAAPNLPPSLQDKPAVPAQVTLRDGSLSVDARNS
                     SLSDILKNVEGSSGMTVDGFGKDSRIFGVYGPGPPRDVLAELLDGAGYNFLMVGSGSK
                     GTPREIVLTARSNAPLSPPSPGSNSQQEEEDETPNYPPPQVVEPPPPPAPVMPPQQQQ
                     RPRTPQEMLQELQRLREQQQQQQQGQPPQ"
     gene            2251..3288
                     /locus_tag="VHE33_19375"
     CDS             2251..3288
                     /locus_tag="VHE33_19375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99670.1"
                     /translation="MDRIFSGSFARFLAAALTLGVAVPAFGVSCMTQSQMASAQRTVL
                     AQSAQMLAANVESGNASAVQAQTIPAVASQFGGIANSIQQVSASIQHAALTVDALYVL
                     DATDLKSAQESQFFCGVPGSSLTVEVTIPNLPPGKYALAIVHATGVKEPQQISMVLQN
                     DPAGSPIWKLAGFFTRPMTMGGHDGLWFWRQARDYAAKKQLGPAYFYYQTAHTLLVPV
                     DFISSPNLQKLDKELEQSRPDALPGQEPMRLNAGAQSFQVTNLHVGEFSGQLDLIVTY
                     SATPGLDPVAARAQVTAVMRALLQQYPGLQSAFHGLWVYAATPGNPNSFALELPMDQI
                     QASVSTSGQRS"
     gene            3333..3980
                     /locus_tag="VHE33_19380"
     CDS             3333..3980
                     /locus_tag="VHE33_19380"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HVT99671.1"
                     /translation="MASRTEKPLSEVIEERRDTPSFDGAPIPDADLKEILEAGLKMPS
                     GFNLQPWRLVVVRTPELQRKLRAACFNQPNVEEASVVIVACGDKDSWRSDMDEMIRMG
                     RERGMTENYAESARSVIPEFMSSHPNLSAWLNRHVMIAFTAMMLMAETLGYDTSSTEG
                     YDPEKVCQILNLPISYEVIALLGIGHLRGVDKYNGGRFSMQRTVFEGEYGKPIKL"
     gene            3977..4414
                     /locus_tag="VHE33_19385"
     CDS             3977..4414
                     /locus_tag="VHE33_19385"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HVT99672.1"
                     /translation="MRREYPEAPIAGVGAVIVRPEADGAKRVLLIRRGQEPLKGEWSL
                     PGGAVELGETLEEAICREVLEETSLAVEVLDTVQAFDRISRDDSGRVRYHYVLIDFLC
                     RVTGGSLACATDAAEARWASADELEGLSKLTVEVIRKALRMAS"
     gene            4438..>5250
                     /locus_tag="VHE33_19390"
     CDS             4438..>5250
                     /locus_tag="VHE33_19390"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVT99673.1"
                     /translation="MATAAAPPQRKIRRAWRTLRWVLLSILTLLVFVVVFAFLRPLTV
                     LWTATQAHLLFHGIHSEYTAIPFAGNPQVRIHYYAGGSGSPIVLVHGLGARAEDWANL
                     MPQLVRDHHRVYALDLPGYGRSDWPRNASYSIPELAGAVEAFMDNRHLSQTDLAGWSM
                     GGWLAMRVALDQPQRIRRLVILDSAGTRFSLVWDTSLFEPDTPAKLAQLDDLLMAGPA
                     PHVPGFIQRDIFRFVGKHAWVVKRNMDSLLTGNDLLDGKLGALKMPMLIVWGK"
BASE COUNT         1019 a         1551 c         1663 g         1017 t
ORIGIN      
        1 cgatttcccc gccatcgaga cctggcgcaa tcagtttcca ggatatgaga tcgtgatcga
       61 cgatccggag ttcacctccg tgtgcccaaa gacagggttg cccgattttg gcgtgatcac
      121 gctgcgctac atgccgcgcg agcagtgcct cgagctgaag tcgtggaagg aatacctctt
      181 cagctatcgc aacctcggca tttttcagga aaacgtcgtc aaccgcatcc tcgaggatgt
      241 ggtcaaagcc tgcgatccgg tgtgggccgt ggtgaagggt gacttccggc cgcgcggcgg
      301 gatctcaacg acgatcgaag cgcgctggcc cagaccgaaa caatagcgga cggggaagag
      361 cgaaagcgaa aacagccttc ctgcttcttt gcctcgagct tccggcattc ctgctactgc
      421 tgcaccgcca gcgaaccgct tcctgaatcg ccgttgcagg ggccgccgga gatcgaccag
      481 ttcgtgatag tcagctggct ggcctgtgca ttgaacgtgc caatggcagt caacttcgtc
      541 gagctgccgg ggtccgaata ggtcatcgcc aaactgctgc ccgtgaccgt cgatgtcgtg
      601 accgaaggct ggtttacaaa gcagctgttg ccgggaaacg acgcagttcc cgacagcgag
      661 aactggccat tctgatccgg ctgcgaagtc tgctcgagga ccgccgaaac cggaatcgag
      721 tcgttcgaat cgtcgacgaa ggtccccgtg tatgttccgt tgatggccga ataatttgtc
      781 gctgtcacag cggctgctcc aagggatgca caactgccgc ccgcgaagct ccaacgggca
      841 gaggcgatgc ccttgccgtc gcccgagacc tgaccctgca gcgtgagagt cgttccgctg
      901 aacggtgagg agatcagcat catcttgttg tccagtccca ccgaacccgt cagcaggacg
      961 tcgctggagg gactgacgca ggagcttccg ttgagccggg caacggcgct gaccgtctcg
     1021 taagccccct gcatgaatcc aacgttcagc accaccccgg atgaggtggg cgcgaaaacc
     1081 cagttgccgg agagcgaggt gccgacctga aggggcgtgt tggccatgcc gcatccggca
     1141 aggagaagtg cgagaccagg aacagagagg agtgcaaggg tacagctgcg gttctgcatg
     1201 gcggagagcc ttttgaatcc tcggaggagg ttattcaaaa gataaacagc ggcccatccc
     1261 gcttccagac cccgaaaggg gcacgactgt tactacggtc atgaattcgg agcagaagta
     1321 cggtttcggg aagccagacc cgccggacga tccaggccag aactttactg gggcggctgg
     1381 ccctgttgct gctgttgttg ctgttcgcga aggcgttgca gctcctgaag catctcctgc
     1441 ggcgttctgg gcctctgctg ctgctggggc ggcatgaccg gcgcgggcgg tggtggcggc
     1501 tccactacct gaggaggtgg ataattgggc gtctcgtctt cctcttcctg ctgtgaatta
     1561 ctgccgggcg agggcggcga aagcggagca ttgctccgtg ccgtcagcac gatctcccgt
     1621 ggggttccct tgctgccgga acccaccatg aggaagttat atccggctcc gtcgagcagt
     1681 tcggccagca cgtcgcgcgg cggaccggga ccgtagacac cgaaaatgcg cgagtccttg
     1741 ccaaaaccat cgacggtcat gccactggag ccctcgacgt ttttgaggat gtccgacaag
     1801 ctggaattgc gcgcatcgac agagagagaa ccatcgcgga gggtgacctg ggcaggcact
     1861 gccggcttgt cctgcaacga tggaggaaga ttcggcgcgg cagcaggctg gggaccgttc
     1921 ggcgcactgg tgacggcacc ggtatgaatt gagggctggc cttgaacttc tggccccggg
     1981 gcggcggcag gaggggtcga aacgccctgg cgtggcggaa aggggaagtg ccgcccaggc
     2041 atgtttccgg ggggctgcga cacctgggcc tgacacgcgg ccgctaaaac ggcggcaacc
     2101 gtcaccataa ggcatcgtga aaggcttgga acctgcatcg aactgtgtgg atttacaggg
     2161 tgccgtgggt ccggccgtta ccccgctcct gtaagtaata gactagcagt gattcgaatt
     2221 cgctcgccgc gaattgaggg gacgtctccg atggatcgca tcttttccgg gtcattcgcc
     2281 cgatttctgg ccgccgccct gaccctgggg gtcgctgtgc cggcttttgg cgtttcctgc
     2341 atgacgcagt cgcagatggc gagcgcccag cgcaccgtgc tcgcccagtc cgcacagatg
     2401 ctggcggcga acgtggaatc gggtaacgct tctgcggttc aggctcagac cattccagcc
     2461 gtcgctagcc agtttggtgg cattgcgaat tccatccagc aggtgagtgc ttcgatccag
     2521 cacgctgcgc tgacagtgga cgccctgtac gtccttgacg cgacggatct gaagtcggca
     2581 caggagagcc agttcttctg cggagtaccg ggctcatcgc tgaccgtcga agtgacaatc
     2641 ccgaatctgc cgcccggaaa atatgccctg gcgattgtgc atgccaccgg cgtgaaagag
     2701 cctcagcaga tctctatggt cttgcagaac gatccggcag gaagcccgat ctggaaactg
     2761 gccggtttct tcactcgccc gatgacgatg ggcggacacg atggcctgtg gttctggcgt
     2821 caggcgcgcg attatgctgc gaagaaacaa ctgggtcccg cgtatttcta ctaccagact
     2881 gcgcatacgc tgctcgtccc ggtcgacttt atttccagtc ccaacttgca aaagctggat
     2941 aaggaactgg agcagtcccg gcccgatgct ctgccagggc aggagccgat gcggctgaac
     3001 gcgggcgccc agagcttcca ggtcaccaat ctccatgtcg gcgagttttc cggccagctt
     3061 gatctgatcg ttacgtacag cgcaacgccg ggccttgatc cggtggctgc ccgcgctcag
     3121 gtcaccgcgg tgatgcgtgc cctgttgcag cagtatccgg gcctgcagtc cgctttccac
     3181 ggtttgtggg tctacgccgc gacccccggc aacccgaact cctttgcgct ggaactgccc
     3241 atggatcaaa tccaggcctc ggtttccacc agcggacaac gcagttaaag tccctctacc
     3301 ctttcaggaa agcaggcgga aaagattccg gcatggccag tcgaacggaa aaacccttaa
     3361 gtgaagtcat cgaggagcgc agggatacgc cgagctttga cggcgcaccg atcccggacg
     3421 cggacctgaa ggaaattctc gaggccgggc tcaagatgcc cagcggattc aacctccagc
     3481 cgtggcgtct tgtcgtggtg cgtacgccgg agttgcaacg caagctgcgt gcagcctgtt
     3541 ttaaccaacc caatgtcgag gaagcgtcgg tcgtgatcgt ggcgtgcggg gacaaggaca
     3601 gctggcgcag cgatatggac gagatgatcc gcatgggacg cgagcgcggg atgacggaga
     3661 actatgccga gtcggcacgc tcggtgattc cggagttcat gagcagtcat cccaatcttt
     3721 cagcctggct gaaccgtcat gtgatgatcg cgttcacggc catgatgctg atggctgaga
     3781 ccctcgggta cgatacttcc tcaactgagg gctacgatcc ggaaaaggtc tgccagattc
     3841 tgaacctgcc gatcagctac gaggtaatcg cgttgctggg gatcgggcac ctgcgaggtg
     3901 ttgacaagta caacggcggc cgattcagca tgcagcgcac cgtcttcgag ggcgagtacg
     3961 gcaagcccat taagctgtga gacgagaata tcctgaagct cccatcgccg gtgttggcgc
     4021 tgtcattgtt cgccctgaag cagacggggc gaaacgcgtg ttactgattc gccgggggca
     4081 ggagccgttg aagggcgagt ggtcgctgcc tggaggcgcc gttgagcttg gcgagaccct
     4141 cgaagaggcg atctgccggg aggtcctcga agaaactagc ctcgcggtcg aggtattgga
     4201 tacggtgcag gccttcgacc gcatctcgcg cgacgactcc gggcgggtgc gctaccacta
     4261 tgtgctgatc gacttccttt gccgggtgac gggcggctcg ctggcctgcg ccaccgacgc
     4321 ggcagaagcg cggtgggcaa gcgccgatga actggaaggg ctgtccaagc ttacagtcga
     4381 agtgattcgc aaagcgctca ggatggcatc ctaaagatcg gagggcatcg cgcacgcatg
     4441 gcgaccgcag cagctccgcc ccaaaggaag atacgacggg cctggcggac gctgcgctgg
     4501 gtactcctca gcatcctcac gctgctcgtt ttcgtcgtcg tcttcgcgtt cctgcggccg
     4561 ctcactgtgc tgtggacggc cacacaggcg catctgctgt tccacggcat tcactccgag
     4621 tacacggcca tcccgttcgc cggaaatcct caggtacgca tccactatta cgccggcgga
     4681 tcgggctcgc cgatcgttct ggtgcacggc ttgggggccc gcgcagaaga ctgggccaac
     4741 ctgatgccac agctggtgcg cgatcaccat cgtgtgtacg cgctcgacct gccaggctac
     4801 ggtcgctccg actggcctcg caacgcctcg tactccatcc ctgagctggc gggcgccgtt
     4861 gaggcgttca tggacaaccg ccatctcagc cagacggatc tggcaggctg gtcgatgggc
     4921 ggatggcttg ccatgcgggt cgcgctcgat cagccgcagc gcatccgcag gctggtgatt
     4981 ctcgacagcg ccggcacacg cttcagcctg gtttgggaca catcgttatt cgagcccgat
     5041 actccagcga aactggctca actggacgac ctgctgatgg ccggacccgc tccgcacgta
     5101 cccggattta tccagcgtga catcttccgt ttcgtgggca agcatgcgtg ggtggtgaag
     5161 cgaaatatgg attcgctgct gaccggcaac gaccttctcg acggcaaatt aggagccctt
     5221 aagatgccga tgctgatcgt ttggggaaaa
//
LOCUS       DASZYN010000757         2649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_305557, whole genome shotgun sequence.
ACCESSION   DASZYN010000757 DASZYN010000000
VERSION     DASZYN010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305557"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VHE33_19395"
     CDS             <1..330
                     /locus_tag="VHE33_19395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99674.1"
                     /translation="LGFTFERLITIDLVLWGMSLALEFLALVVLRKREPLLARPFRVP
                     GPDWFAVVLGLSPMALTVYALYASRSETVAGMSAFAFSLTIAVMGVPLYLLAAWSRKR
                     HATAQAS"
     gene            complement(367..2196)
                     /locus_tag="VHE33_19400"
     CDS             complement(367..2196)
                     /locus_tag="VHE33_19400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HVT99675.1"
                     /translation="MPPIVNAQNISKRFGATPLFENIGFAVHDGDRIGLVGPNGAGKS
                     TLLAILGAEQEPDSGEVAFRKRARVGYVHQISDFAPGATVRSIVESALARTGVPESSR
                     EQRLRETLGRAGFFESDTSGVSFSAEAATLSGGWRKRLAIAEGLVTEPDLLLLDEPTN
                     HLDIEGIEWLEGLLQDAPFAFVVVSHDRRFLENTAHAIVELNRIYSDGLLRVQGAYSR
                     FLEEREAWLESEQRAQESLRNRVRTEIEWLRRGPKARATKAKARIDNANALIAQLQDS
                     ESRSRSLTAGIAFDATDRQTKRLIEMEDAAVTLGDREILRGVTLSVVAGLRLGLVGPN
                     GSGKTTLLRALTGELPLSSGLLRRAPALRIVYFSQLRELDESLTLRRALAPDSDSVLY
                     QDRVVHVASYASRFLFSGEQLNQPVSRLSGGERARVLIARLMLQPADVLILDEPTNDL
                     DIPTLEILEEALLDFPGALLLVTHDRYLLDRVTNAVLGLDGRGNAAIFADYIQWEEWR
                     AQLSAPGKSPAPEPRPASPAPRTPGPKKKLSYLEQREYDTIEARIEEADQRLLRARAR
                     VDDPAVTIDAAALTQALSELEAARSEHDAVLERWLELTEKTGG"
     gene            complement(2263..>2649)
                     /locus_tag="VHE33_19405"
     CDS             complement(2263..>2649)
                     /locus_tag="VHE33_19405"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HVT99676.1"
                     /translation="AIGDFDNDGAVDVLIAVNDAAPLLLRNRAARGRHWLGLTLVGRQ
                     SNRDAIGARVTWQAGDLTRSRTKTAGGSFLSSHDPRFVLGIGSRTQIDWLEVHWPQPG
                     GKVERFTKLPIDRYITIVEGEGRVTL"
BASE COUNT          454 a          731 c          971 g          493 t
ORIGIN      
        1 ctggggttca cgtttgagcg gctgatcacg atcgacctgg tgctgtgggg catgtcgctg
       61 gcgctggaat tcctggcgct ggtggttctg cgaaaacggg agccgttgct ggcgcggccg
      121 tttcgtgtgc ccgggccgga ctggtttgcg gtagtgctgg ggctgagtcc gatggctctg
      181 accgtttatg cgctgtacgc ctcgcggtcg gagaccgtag cggggatgtc agcctttgct
      241 ttttcgctga cgatcgcggt gatgggagtg ccgctgtatc tgctggcagc gtggtcgaga
      301 aagcgtcatg caacggcgca ggcaagctga cgcatgggaa gcgcagtctc agccgcccgc
      361 gatttgctac ccgccggtct tctctgttag ttccagccag cgctccagca ccgcatcatg
      421 ctcggaccgc gccgcctcga gctcagagag cgcctgggtg agcgcagcgg catcgatggt
      481 gacggcggga tcatcgacgc gggcgcgagc ccggagcagg cgctggtcgg cttcttcgat
      541 gcgggcctcg atggtgtcgt attcgcgctg ttccagatag gagagcttct ttttgggacc
      601 gggagtgcga ggggcgggcg acgcggggcg cggttccggt gcaggcgatt tgccaggggc
      661 agagagttga gcgcgccact cttcccactg gatgtagtcg gcaaagatgg cggcgttacc
      721 gcggccgtcg agtcccagaa ctgcgttggt gacacggtcg agcaggtagc ggtcgtgggt
      781 cacgaggagg agagcacccg gaaaatccag cagcgcctcc tcgagaattt caagcgtggg
      841 aatgtcgagg tcgttggtgg gctcgtcgag gataaggacg tcggcgggct ggagcatgag
      901 gcgggcgatg agcacgcggg cacgttcgcc accgctgagg cgggagaccg gctggttcag
      961 ctgttcgccg gaaaagagaa accgggaggc gtagctggcg acgtggacga cacgatcctg
     1021 gtagaggacg gaatctgaat ccggcgcgag cgcgcgacgg agggtcagcg actcgtcgag
     1081 ttctcggagt tgggagaagt agacaatgcg gagagcgggc gcgcgccgca gcagaccgga
     1141 agagagcggc agctcgccgg tgagggcgcg caggagcgtg gtcttgcccg agccattggg
     1201 cccgacgagg ccgagacgaa ggccggcgac gacgctgagg gtgacaccgc ggagaatctc
     1261 gcgatcgccc agggtcacgg cggcatcttc catttcgatg aggcgcttcg tttggcggtc
     1321 ggtcgcgtca aaggcgatgc cggcggtgag agaacggctg cgggattcgg aatcctgcaa
     1381 ctgggcgatg agcgcgttgg cgttgtcgat acgcgctttg gccttggtgg cccgcgcttt
     1441 agggccgcga cgaagccact cgatctcggt gcggacgcgg ttgcggaggc tttcctgcgc
     1501 gcgctgttcg gattcaagcc aggcttcgcg ctcctcgagg aacctggagt aagcgccctg
     1561 gacgcgcagc aggccatcgg agtagatccg gttcagttcg acgattgcgt gggcggtgtt
     1621 ttcaaggaag cggcggtcgt gcgagaccac gacgaaggca aagggcgcgt cctgcagcag
     1681 gccttcgagc cattcaatgc cttcgatgtc gagatgattc gtgggctcat cgagcaggag
     1741 caagtcgggt tcggtgacga gcccttcggc gatggcgagg cgtttgcgcc atccgccgct
     1801 cagtgtcgct gcctcggcag agaaggaaac gccggaggta tcggactcga agaagccggc
     1861 gcggccgagg gtttcgcgca ggcgctgctc gcggctgctt tccggcactc cagtgcgagc
     1921 caaggcggat tcgacgatgg atcggacggt ggctccgggt gcgaaatcag agatctgatg
     1981 aacgtagcca acacgggcgc gcttgcggaa ggcgacttcg ccggagtcag gctcctgttc
     2041 ggcgccgaga attgccagga gcgtggattt gccggctcca ttggggccga caaggccgat
     2101 gcggtcgccg tcatggacgg cgaacccgat gttctcaaaa agaggcgtgg ccccgaatcg
     2161 tttggagatg ttctgggcgt tgacgatcgg cggcatgctt tcagtttatg ggttcagcag
     2221 gcagttgctg cccgccgcag gccgcccggt gcgatcaaac tcttacaaag tcacccgacc
     2281 ttctccctca acgattgtga tgtacctgtc gattggcagt ttggtgaacc tctcgacttt
     2341 gccaccgggc tgcggccagt gcacctcgag ccagtctatt tgggtgcggg agccgatgcc
     2401 gagcacgaat cgcggatcat gagaggagag aaaacttcct cccgccgttt tcgttctgct
     2461 gcgcgtgaga tcgcctgcct gccaggtgac gcgcgcgccg atggcgtcac gattcgattg
     2521 ccggcctacc agggtgaggc cgagccagtg acggccgcgg gcagcgcgat tgcgcaaaag
     2581 gagaggggca gcatcgttga cggcgatgag cacatcgacg gcaccgtcgt tgtcgaagtc
     2641 tccgatcgc
//
LOCUS       DASZYN010000758         1866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_110941, whole genome shotgun sequence.
ACCESSION   DASZYN010000758 DASZYN010000000
VERSION     DASZYN010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110941"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="VHE33_19410"
     CDS             complement(<1..1164)
                     /locus_tag="VHE33_19410"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT99677.1"
                     /translation="MSHDLRFALRMILAHRWFSLAVVVTLALGIGLNTMVFTIINAAL
                     FKPVPVPGGDRLVSIATHTADGRDRRMSYPYFLDIRAQSHSFEGIEAGANQEGVLGEP
                     GIPPQSYHAQRVTTGIFSMLHMHPILGRDFLPSDGQPGAAPVLMIGYQVWQERYAGSL
                     SVIGRQVRMNEKPATIIGVMPKAFGFPTTVDLWMPLVPTPDDMKRDNRDLEVFAILRP
                     GVKIAVAATEIHALSLRIASQYSGADKDTTANVMSFHERYNGGNIKMVFLLMLASVGF
                     VLLIACANVANMMLSRAIARQREMSIRAALGASRWRVMRQLLLESVVLSVLGGALGLG
                     LAAFGVYWFNLATSNVGKPYWVQFTMDYPVFGYFAALCIVSGLLFGILPALRAS"
     gene            complement(1298..1594)
                     /locus_tag="VHE33_19415"
     CDS             complement(1298..1594)
                     /locus_tag="VHE33_19415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99678.1"
                     /translation="MQFTQRLREGVRTGQITCSVRIWTRPHVRVGGRYRMEEGEIEID
                     SIEPIGLPDITPELARASGFLGVLDLLKVAKHGAGQNIYLIRFHYIPPRRKKRA"
     gene            1676..>1866
                     /locus_tag="VHE33_19420"
     CDS             1676..>1866
                     /locus_tag="VHE33_19420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99679.1"
                     /translation="MTAKKATRAAKKNPPNRATKKAPEKKKAGRGAAGGVEDYIAGIP
                     ESSRKTFDALRAAARQAAP"
BASE COUNT          400 a          502 c          668 g          296 t
ORIGIN      
        1 tgaggcgcgc agcgcgggca ggatgccgaa gagcaggccg ctgacgatgc aaagcgcggc
       61 gaagtacccg aagacggggt agtccatggt gaactgcacc cagtaaggct tcccgacatt
      121 cgatgtggcg agattgaacc aatagacgcc gaaggcggcg aggccaaggc cgagggctcc
      181 gccgaggacg ctgaggacga cactctccag cagcagctga cgcatgacgc gccagcgaga
      241 cgcgccgagc gcggcgcgga tggacatctc gcgctggcgg gcgatcgccc ggctgagcat
      301 catgttggca acgttggcgc aggcgatgag gagcacgaac ccgacggaag ccagcatcag
      361 caggaacacc attttgatgt tgccgccgtt gtagcgctca tggaagctca tcacgttggc
      421 ggtggtgtcc ttatcggcgc cggagtactg ggaggcgatg cgaagagaca gggcatggat
      481 ttccgtcgct gcgacggcga ttttgacgcc tggccgaaga atggcgaaga cttcgaggtc
      541 ccggttatcc cgcttcatat cgtccggagt ggggacgagg ggcatccaga ggtcgacggt
      601 ggtggggaaa ccaaacgctt tgggcatgac gccgatgatg gtggcgggct tttcattcat
      661 gcgcacctgg cggccgatga cggagagcga gccggcatag cgttcctgcc agacctggta
      721 accgatcatg agaaccggcg cggcgccggg ctggccgtcg gaaggcagaa aatctctccc
      781 cagaatcggg tgcatatgga gcatggagaa gatgccggtg gtgacgcgct gggcgtgata
      841 cgactggggc gggattcctg gctcgccgag caccccctcc tgattggcgc cggcttcgat
      901 tccttcgaac gaatgggact gggcgcgaat atcgaggaaa tagggatacg acatgcgacg
      961 gtctcgaccg tctgccgtat gcgtcgcgat ggaaaccagg cgatccccac ccggaaccgg
     1021 taccggcttg aacagggctg cattgatgat ggtgaacacc atcgtgttca acccgatgcc
     1081 gagcgccagc gtgaccacga cggcgaggga gaaccagcga tgggcgagga tcatgcgcag
     1141 ggcaaagcgg agatcatgcg acatgcctgg gatacggagc ggggcctggg atggttccgg
     1201 gaatgtgatg aacaagacgc gggaagcagg gcgcagatcg cagggcggag cgagttcgcg
     1261 agtcagcagg tcagcgagtc agcgctcgaa aatgtcgtca ggcccttttt ttacggcgcg
     1321 gcgggatgta gtggaagcgg atgagataga tgttctgccc agcgccatgt tttgcgactt
     1381 tcagcaggtc gaggacgccg aggaaacccg aggcccgtgc cagctcgggc gtgatgtcgg
     1441 gcaggccgat gggctcgatg gagtcgatct caatctcgcc ttcttccatg cgatagcggc
     1501 cgccaacccg cacgtgagga cgggtccaga tgcggaccga gcaggtgatt tggccggtgc
     1561 ggacgccttc gcgcaggcgc tgggtgaact gcatgggaac gagtctatgc gatgcgggct
     1621 gggcttcaag gatgtagaga tggatttgcc ggaggccagg cgttaggatc ggctcatgac
     1681 ggcgaaaaag gctacccggg cggcgaagaa gaacccaccc aaccgcgcga cgaagaaagc
     1741 tcccgaaaaa aagaaagcag gccgcggagc tgccggcggc gtcgaggact acatagccgg
     1801 aattccggag agcagccgga agactttcga tgctcttcgt gcggcggcgc gccaggcagc
     1861 gccaaa
//
LOCUS       DASZYN010000759         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_687673, whole genome shotgun sequence.
ACCESSION   DASZYN010000759 DASZYN010000000
VERSION     DASZYN010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687673"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VHE33_19425"
     CDS             complement(<1..869)
                     /locus_tag="VHE33_19425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99680.1"
                     /translation="MTLSWTLRLLCLLLVVTGLSLALSQLALALASRSILRRLDSLTA
                     RWRERILYLLQIGPALFAAVVAAALCLPAYLRGETNVESERVGILFLLAAALTAFWFG
                     QALLRGLRITLRTLRFARTCRRSGQRLQHNSDIPLLAVPDSGPPVRLIGFLHPLILVS
                     PSMSTLAPAALDLAIAHERSHAAHRDNWKLLTLSFLPRPLPGGDPWNRPWHRAADWAA
                     DDDAVRGDSTRSLLLAETLITVARAANSSRAPYICTALATADAALAVRIDRLLHPRHD
                     LRSSGSSLLIGLAV"
     gene            complement(866..1348)
                     /locus_tag="VHE33_19430"
     CDS             complement(866..1348)
                     /locus_tag="VHE33_19430"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HVT99681.1"
                     /translation="MKRPERPSGHGTTNTSSPATLGILGRREREVLSVLRQLGSATVQ
                     QVAERLETSLAYTTVMTTLDRLFRKGLLTRRKQNRAFLYSSSLSARDFEGRRAAHFIR
                     RFFSESDVQPEVLVSCLVDAVHRYDTELLDQLESSIRTARAETDQAQPSPPNKQEGQL
                     "
     gene            1512..>2719
                     /locus_tag="VHE33_19435"
     CDS             1512..>2719
                     /locus_tag="VHE33_19435"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT99682.1"
                     /translation="MKKLLWTLLVFSPVCVIGQSPDSAAVRGRVTGPNGQVVSGTRVA
                     IEDTQHHVVRSVVPGPDGDFAVEGLAPETVMVRADAQGLEASSGELTLAAGTRAMVEL
                     HLHVPSVHVAVEVTGVVGEVRTDEPQLGDRLTAEQAQAMPLPNRRITFLPLVNAANHP
                     AINQGDVFMNQMLFTANGTGRRQTWFEVNGANGNDMWGRQTIFTNVPLSAIGEMTVLE
                     NAFAADYGFGEGAVVNVVTRSGGSRFHGNVLGLWRPSGPEAALSGFTAKNAASGNDIT
                     RDTLAQGAATVSGPVTKEGQTYYTASAEYSYQDRASPVISPLAPGNYIGHYRDWMGLL
                     RLDHTIREGQRVFAFLSADGFFDTNPNGTVGGNNLPSVARVFHRKTYTGELGDTAVLN
                     PTMVNEARVE"
BASE COUNT          548 a          648 c         1070 g          453 t
ORIGIN      
        1 acggcgaggc caatgaggag cgaggagcct gaagatcgca ggtcgtgacg tggatggagg
       61 agacgatcga tgcggacggc gagcgcggcg tcggcggtgg cgagtgcggt gcagatgtag
      121 ggcgcgcggc tggaattggc ggccctggcg accgtgatga gggtttcggc aagaagaagg
      181 gagcgagtgg agtcgccacg aacggcatcg tcatcggcgg cccagtcagc tgcacggtgc
      241 cagggccggt tccagggatc gccgccggga agcgggcgag gcagaaaact cagagtgagg
      301 agcttccagt tgtcgcgatg ggctgcatgg gagcgctcat gagcgatggc cagatcgagg
      361 gctgcggggg cgagggtgct catcgacgga gatacgagga tgagggggtg gaggaatccg
      421 atgaggcgga ccggtgggcc cgaatcgggg acagcaagga gaggaatgtc gctgttgtgc
      481 tgaaggcgct gaccggagcg gcggcaggtg cgggcaaaac gaagagtgcg gagggtgatg
      541 cgcagtccgc gcagcagagc ctggccgaac cagaaagcgg tgagggccgc agcgaggagg
      601 aacaggatgc cgacgcgttc ggattcgacg ttggtttcgc cacggagata tgccgggagg
      661 caaagcgccg ccgcgacgac ggcggcaaac aaggcggggc cgatctgaag gagatagagg
      721 attcgttcgc gccagcgcgc tgtgagggaa tcgaggcgac ggaggatcga gcgagaggcg
      781 agcgcgagcg cgagctgtga aagcgcgagg gagagcccgg tgacgacgag aagaaggcag
      841 aggaggcgca gagtccagga cagggtcata gctggccctc ctgtttgttg ggcggagatg
      901 gctgggcctg atcggtctca gcacgggcgg tgcgaatgct ggattcaagc tgatcgagga
      961 gttcggtgtc gtagcggtgg accgcgtcga ccaggcagga gacgagaact tccggctgaa
     1021 cgtcggattc agagaaaaag cggcggatga aatgggcggc gcggcggcct tcgaaatcgc
     1081 gcgcggaaag agacgaggag tagagaaagg cgcgattttg cttgcggcgc gtcagcaggc
     1141 ccttgcggaa gagacgatcg agagtcgtca tcacggtggt gtaggcgagg ctggtctcga
     1201 ggcgctcggc aacctgctgg acggtggcgc tgccaagctg gcggaggacc gagagcactt
     1261 cgcgctcgcg gcgaccgagg atgccgagag ttgccggcga tgaggtgttg gtcgtgccgt
     1321 gaccggatgg tcgttcaggt cgtttcattg gggggtccga aggagacttc gatgatagcc
     1381 cgaaaagacg ggtgaagtga aagagcccga ggcggatgca ctcctactac ctctggtagt
     1441 agtcgaagga atcccgtagt tctgaagatg caagccctcc cccattcccc ggatcaacat
     1501 tgcggagaga tatgaagaag cttttgtgga cattgctggt tttttccccg gtctgcgtta
     1561 ttggacaaag cccggacagc gcggcggtgc gcgggcgcgt cacgggaccg aatggccagg
     1621 ttgtgagcgg gactcgtgtg gcgatcgaag atacgcagca tcacgtggtg cggagtgtgg
     1681 tgccggggcc cgatggggat ttcgcggtgg aagggctggc gccggagacg gttatggtgc
     1741 gcgctgatgc gcagggcctc gaggcgagtt caggggagct aaccctggcc gcggggacca
     1801 gggcgatggt tgaactgcat ttgcatgtgc cttcggtgca cgtggcagtt gaggtcacgg
     1861 gtgtggtggg cgaagtgcgg acggacgagc cgcagctcgg ggatcgtttg accgctgagc
     1921 aggcgcaggc gatgccgctg ccgaaccggc gaatcacttt tctgccgctg gtgaatgcgg
     1981 cgaatcatcc ggcgatcaat cagggcgacg tgttcatgaa ccagatgctg tttaccgcaa
     2041 atggcacggg gcggcggcag acgtggtttg aagtgaatgg cgcgaatggg aatgacatgt
     2101 ggggtcggca gacaatcttc acgaacgtgc cgctgagcgc gattggggag atgacggtgc
     2161 tggagaacgc gttcgccgcg gactatggat tcggcgaagg cgctgtcgtg aatgttgtga
     2221 cgcgcagcgg gggcagtcgt tttcacggga atgtgctggg actgtggcgg ccgtcgggtc
     2281 cagaggcggc gctctcggga ttcactgcaa aaaacgctgc gagcgggaat gacatcacgc
     2341 gggacacgct ggcgcagggg gccgcaactg tatccggtcc ggttacaaaa gaggggcaga
     2401 cctattacac ggcgtcggcg gagtacagct atcaggaccg ggcttcaccg gtgatttcgc
     2461 cgctggctcc aggaaactac atcgggcact atcgcgactg gatgggactg ctgcgtctgg
     2521 atcacacgat ccgggagggg cagcgcgtgt ttgcgtttct gagtgcggat ggatttttcg
     2581 atacgaatcc caacggcacg gtgggcggaa acaacctgcc gagcgtggcg cgcgtgtttc
     2641 atcgcaagac gtacacgggt gagttgggcg atacggcggt gctgaacccg acgatggtga
     2701 atgaggcgcg cgtcgagtt
//
LOCUS       DASZYN010000760         5515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_746873, whole genome shotgun sequence.
ACCESSION   DASZYN010000760 DASZYN010000000
VERSION     DASZYN010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746873"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3952
                     /locus_tag="VHE33_19440"
     CDS             <1..3952
                     /locus_tag="VHE33_19440"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HVT99683.1"
                     /translation="YSIDAAGRVTQAHHLVYRIETAAGLDHWSEVSSEWETFFQNQPI
                     LRARVIRSDGSQLDLDPKTATDVAASDRNDGTLSDDRIYKAPLPGLAIGSIVEMETTN
                     IDKEPFFADGGLYRIYLQRGVPITRSRVTVSLPASLPFTYKIHHLPDLITKDEKTGDL
                     HNVTFDEGRLAPGVSSDIELATPSPLWPEIEFATGKSWHDIAAAYDRMVEPQIRLDQV
                     RQLLPNKTPDRLARIQQLVARLHKEVRYTGIEFGRAKLEPRTPEEILSRHYGDCKDKA
                     TLLVAMLRATGIDAHLALLDTGPGMDVTPDLPGIGDFDHAIVFVPATANDPALWIDAT
                     AEFTRVGDLPWADQGRLALIVAGNTQDLTRTPDARPEDSSVIETREFHLADFGPAHVI
                     ESSDTAGHIDANFRAYYQDTENKNTRDNLENYVKSAYSAKDLKSIQHQDARDLNKPFL
                     LRLDIEKAARGNTGMGDAAVGLDPRGTWNGLPAWFGKDPDRGNEKLSPEQQEAQRKAI
                     AQRATDYQIEPFVCERRYRIFLPPGFVPRALPPDQTIPMGPASFSEKYASQPDGTVTA
                     VFRFSTGSGRYSQDEALALRKAIVDAGKRDWPVLIFDQAGAKLLSQGKTREALAADRD
                     LIAAHPHDAVQLARLARALVDSGAGQLARDEARKATQVDPSSAVAWDSLAWVLQYNDI
                     GVFHGMGFDLDGALAAYRKAEELDPDNVETRENLAILDEHDNQGTRYVSIPGLEHAIT
                     EYRNLKAQNKAAGDAYEDNLLFCLLYAHHYKELKSELASLPASPTRNSLSIAGETASD
                     GVDAGMKAADRLNGDAQQRGTALRNAGQQLIRLRLYPEAAKILAAGVQNQEQSSDTAR
                     QIEVYRDLHPRDLSQAAPSDPGTVVRQMMDALLSGHLTDDDIARMLSRHSFATDEQWK
                     TNLKKMHQATNATFVVTERAGLTPDVLADLTLGNMKITAQGTDQTGYRVALQSIGAAP
                     RLFFITKEEGVFRIVAGQSDMMEVGNQALFLLHAGNEAEARALLDWKRDQMHKGGGDD
                     PLDGPLFPLFWTSGDSTGPDAIERAAAALMAAQMDISAMLPEIESKAKASSDKSKVDP
                     SALNLLLAAGYLRAGKPNLAWPAIEALLRAYPDSVTAARLAGEYYRRVQNWAAWDAML
                     APQVEKHPGNREWLLLKSYSQQSRGDWAAAQHTMRQVLDGGQATATDYNSYAWNALFE
                     SKPEDDALRAGQQTTAMSQNSDFASLHTLACLYAVLGKTREARQVLLQAMQAGSLAEP
                     DPEVWFAMGLVYENDGLSDAAIAAYRRVEKPDAPLNPTDTWVLAQKHLQQLHAL"
     gene            complement(3985..4812)
                     /locus_tag="VHE33_19445"
     CDS             complement(3985..4812)
                     /locus_tag="VHE33_19445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99684.1"
                     /translation="MRRYCAAILLVAFAGAALHGQRTRTPSLDEILQRLETNLRHYDR
                     GVPNFFCDEHALSSQVGRNPPDQKTVTDSVFRLKRTANPDHTTTLAESREIRSVNGKP
                     PTSQRMDGPSVLSGIFEGGLAVVSQNQTACMKYQLQRGERIRRTDPYLIHFATILNPQ
                     NAAGCFLQEKSQGWAYVDPASMQVTHLEISTPRHVIVAGDSDAPPVIGMRKIVADFAP
                     VSLGDEIFWMPSAITMRNVSGSGTFHMMVWSFRATYRNYHRMEVTSRIVPGSEAPVR"
     gene            5043..>5515
                     /locus_tag="VHE33_19450"
     CDS             5043..>5515
                     /locus_tag="VHE33_19450"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVT99685.1"
                     /translation="MRLRWFAVFALLATLCSITASAQIAADLRGTVLDPSGHAVPHAQ
                     VDLTQAATNIHLSTVTSASGDYSFTNLTPGVDRLDVTAPGFAHLTRSGVQAIVGQTVQ
                     LNLTLTLGENRQTIRVTDDAPILQSQTSNIATNIAGSTVIAMPLNTRNFVQLATLA"
BASE COUNT         1154 a         1894 c         1438 g         1029 t
ORIGIN      
        1 ctactccatc gatgctgccg gacgcgtcac ccaggcgcat catctggtgt atcgaatcga
       61 gacagccgcc ggcctcgacc actggtccga ggtctcgtcc gaatgggaga ccttcttcca
      121 gaatcagccc atccttcgcg ctcgcgtcat tcgctccgat ggctcgcagc ttgacctcga
      181 tccaaaaacc gcaaccgacg tcgccgccag tgacaggaac gacggcacgc tttccgatga
      241 ccgaatttac aaagccccgc tccccggcct cgctatcggc agcattgtgg agatggaaac
      301 caccaacatc gacaaggagc cctttttcgc agacggaggc ttataccgga tctacctgca
      361 gcgcggcgtg cccattaccc gatctcgtgt cacagtctct cttcccgcca gcctcccgtt
      421 cacttataag attcaccacc ttcccgacct catcaccaaa gacgagaaaa caggcgacct
      481 ccacaacgtg acgttcgacg aggggcgcct cgctcctggt gtcagctctg atattgagct
      541 ggctacacca tcgcctctgt ggcccgagat cgaattcgct accggcaaat cctggcacga
      601 catcgccgcc gcttacgatc gaatggtcga gccgcagatt cggctcgatc aggtcaggca
      661 gcttcttccc aataagactc ccgatcggct tgcccgcatc cagcagttag tcgcccgtct
      721 ccacaaagaa gtccgttaca ccggcatcga attcggccga gccaaactcg agccgcgcac
      781 tccggaagaa atcctcagtc gtcactacgg ggactgcaaa gataaggcga ctctgctggt
      841 tgctatgttg cgagccaccg gaatcgacgc ccatctggct ctcctcgata ccggccccgg
      901 aatggatgtc acgcccgatc tccccggcat cggcgatttc gatcacgcca tcgtctttgt
      961 gcccgcaacc gcgaacgacc ccgctctctg gatcgatgcg actgccgaat tcactcgcgt
     1021 gggagacctt ccctgggccg atcagggccg tctcgccctc atcgttgccg gcaacaccca
     1081 ggacctcacc cgcacccctg acgcccgtcc tgaggacagc agcgtcatcg aaacccgcga
     1141 attccacctc gccgatttcg gtccggccca cgtcatcgaa tcctccgaca ccgccggcca
     1201 catcgacgcc aacttccgcg cttattacca ggacaccgaa aacaaaaaca cccgtgacaa
     1261 tctggagaac tacgtcaaga gcgcctacag cgccaaagat ctgaaatcga tccagcatca
     1321 ggacgccaga gacctcaaca agcccttcct cctgcgcctc gatatcgaga aggccgcacg
     1381 aggtaacacc ggaatgggtg acgccgcggt tggcctggac ccgcgtggaa cctggaatgg
     1441 tttgcccgcc tggtttggga aagatcccga tcgaggaaat gaaaagctca gccccgagca
     1501 gcaggaagcg cagcggaaag ccatcgcgca gcgcgccact gactaccaga tcgagccatt
     1561 cgtctgcgaa agacgctacc gcatttttct cccgccagga tttgtgccgc gcgcgctgcc
     1621 cccggaccaa accatcccta tgggccctgc ctcgttctcg gaaaaatatg cctcccagcc
     1681 cgatggcacc gtcaccgcgg tatttcgatt cagcaccggc tcaggccgat actctcagga
     1741 tgaggcgctt gccctccgca aggcgattgt cgacgcgggc aaacgggact ggcccgtcct
     1801 catctttgat caggccggcg caaagcttct ttcccaggga aaaaccaggg aagccctggc
     1861 cgccgatcgt gatctgatcg cggcacaccc tcatgatgct gtgcaactcg ctcgccttgc
     1921 ccgcgctctg gtcgactccg gtgccggtca gctggcgcgc gacgaagcca gaaaagccac
     1981 gcaggtcgac ccttcgtcag ccgtcgcgtg ggattctctt gcatgggtcc tccaatacaa
     2041 cgacatcggc gtcttccacg gcatgggctt cgatctcgat ggagccctcg ccgcctatcg
     2101 caaggcggag gaactcgatc cggataacgt cgaaacccgc gaaaatcttg ccattctcga
     2161 tgagcacgac aaccagggaa cgcgctatgt ctccattccc ggcctcgagc acgcaatcac
     2221 ggaatacagg aaccttaagg cacagaacaa agccgccggc gatgcctatg aggacaatct
     2281 cctcttttgc ctcctctacg cgcaccatta caaggagctg aaatccgagc tggccagtct
     2341 gccggcttcg cccacccgca acagtctttc catcgccggc gaaaccgcca gcgatggggt
     2401 tgacgccgga atgaaggccg ccgaccgctt gaacggcgac gcacagcaac gagggactgc
     2461 gctgcgcaat gccggtcagc agctgatccg gctccgcttg tatccggaag ccgccaaaat
     2521 tctcgcggcc ggcgtccaga accaggagca gtcctctgac acggcaaggc agatcgaggt
     2581 ctaccgcgat ctgcatcccc gcgatctcag tcaggctgcc cccagcgatc ccgggaccgt
     2641 cgtgcgccag atgatggacg ctctcctctc tggccacctc accgacgacg atatcgcccg
     2701 catgctcagc cgccactcct tcgccacgga tgagcagtgg aagaccaatc tgaagaaaat
     2761 gcaccaagcc acaaatgcca ccttcgtggt tacagaacgc gccggtctca cgcccgatgt
     2821 ccttgccgac ctcacgttag gcaatatgaa aatcaccgcg cagggcaccg accagaccgg
     2881 ttatcgcgtg gccctgcagt ccatcggcgc tgcacctcgc ttgtttttca ttacgaagga
     2941 ggaaggtgtc ttcaggattg tggccgggca gagcgacatg atggaggtcg gcaaccaggc
     3001 attgttcctc ctccacgcag gcaatgaagc cgaagcccgt gccctgcttg actggaaacg
     3061 cgatcaaatg cacaaaggcg gcggagacga tcccctcgac gggcctctct ttcccttgtt
     3121 ctggacttca ggagattcca ccggtcccga tgccatcgag cgggctgccg ccgccctcat
     3181 ggccgcgcag atggacatct ccgctatgct ccccgagatt gagtcgaaag caaaagcgtc
     3241 cagcgacaag agcaaggtgg atccctccgc gctcaatctt ctgctcgccg cagggtatct
     3301 tcgcgccggc aagccaaatc tcgcctggcc cgccatcgag gctctgctca gggcttatcc
     3361 ggattcagtc acggccgctc gtcttgcagg cgaatattat cgccgtgttc agaattgggc
     3421 agcgtgggat gcgatgctcg cgccccaggt cgaaaagcat ccgggcaatc gcgaatggct
     3481 ccttctcaag tcttattctc agcagtcgcg tggcgactgg gcggcggcgc aacatacgat
     3541 gcgccaggtc ctcgatggcg gccaggcaac cgcgaccgac tacaactcct acgcctggaa
     3601 tgcgctcttc gaaagcaagc cggaggacga tgccctccgc gccggccagc aaacgaccgc
     3661 catgagtcag aactccgatt tcgccagcct gcacacgctc gcctgcctct acgccgtttt
     3721 ggggaaaacc agggaagcgc gtcaggtgct gctccaggcc atgcaggccg ggagtctggc
     3781 cgagccggat cccgaagtgt ggtttgccat gggtctcgtt tacgagaacg acggcttgtc
     3841 cgatgcggcc atcgcggcgt atcggcgcgt cgaaaagccg gacgcgcctc tcaatccaac
     3901 cgacacctgg gttctggcgc aaaaacacct tcagcagttg cacgccctct gatcagccga
     3961 tcgttgcggt gatcggaatc gcggtcaccg caccggcgcc tcgctcccag gcacgatgcg
     4021 cgacgtcacc tccatcctgt ggtagttgcg ataggtcgcc cgaaacgacc aaaccatcat
     4081 gtgaaaggtc ccggaaccac tgacattgcg catcgtgatt gccgaaggca tccagaaaat
     4141 ctcatcgcca agcgagaccg gcgcaaaatc cgccacaatt ttccgcattc cgataaccgg
     4201 cggcgcatcc gaatcacccg cgacgatgac gtggcgcggc gtggagatct ccagatgcgt
     4261 tacctgcatc gaggcaggat cgacatacgc ccatccctgg ctcttctcct gaagaaaaca
     4321 gcctgcggca ttctgcggat tgagaatcgt ggcgaaatgg atgagatagg ggtccgtccg
     4381 cctgatccgc tcgccccgct gcaattggta cttcatgcag gccgtctgat tctgggagac
     4441 tacggcaagt ccgccctcaa agattccgct taacacactc ggtccgtcca tcctttgtga
     4501 agtcggcggt ttgccgttca cgctcctgat ctcgcgcgac tctgcgagag tcgtcgtgtg
     4561 gtctggattc gccgtcctct tcagtcggaa gaccgagtcc gtgaccgtct tctgatcggg
     4621 tgggttgcgc cccacttgcg atgacagagc atgctcatcg caaaagaagt tcggaacgcc
     4681 gcggtcgtaa tgtctcagat tcgtttccag ccgctgaaga atctcgtcca gactcggcgt
     4741 tcgcgtccgc tgcccatgaa gcgccgcccc agcaaaagca acaagcaaaa tggccgcgca
     4801 gtatctccgc atcaatatag gttcccgaac atatagaccc acagctgaca aaaccgtaat
     4861 cgtcctttcc cagcctcgcc ggcatcccgg caaacccgtc acagatcgta ttgacctgac
     4921 gagttgaacc atataaattc ggaatatcgt tcgtgctgtc cctgtagctt ctcgtcccgc
     4981 tcccggatct ccattcgctt cggcccgaac agaattgagg ccttacctcc cccggagaat
     5041 gcatgcgtct gcgctggttc gctgtgtttg ccctgctcgc gaccctctgc agcatcacag
     5101 ccagcgccca gattgccgct gacctgcgcg gaaccgttct cgatccctcc ggtcacgccg
     5161 tgccacacgc tcaggtcgac ctcacccagg cggccaccaa tattcacctg tccacagtga
     5221 cttcggcctc aggggattat tccttcacca acctcacgcc cggcgtcgat cgcctcgacg
     5281 tcaccgcccc aggattcgcc cacctcaccc gctccggcgt tcaggccatc gtcggccaaa
     5341 ccgtccagct caatctcacc ctcaccctcg gcgaaaaccg gcaaaccatc cgcgtcaccg
     5401 acgacgcgcc catccttcag tcccaaacca gcaacatcgc caccaatatc gccggatcca
     5461 ccgtgatcgc gatgccgctc aacacgagaa atttcgtcca gctcgctacc ctcgc
//
LOCUS       DASZYN010000761         1661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_177838, whole genome shotgun sequence.
ACCESSION   DASZYN010000761 DASZYN010000000
VERSION     DASZYN010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177838"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..>1661)
                     /locus_tag="VHE33_19455"
     CDS             complement(240..>1661)
                     /locus_tag="VHE33_19455"
                     /inference="COORDINATES: protein motif:HMM:NF012859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HVT99686.1"
                     /translation="WCTRPALRVNGQSVSASAGPAFVTINRTWASGDTVTLQLPMQTS
                     VQTWANNHNAVSVNYGPLTFALQIGEDYTLINNSTAQVPEYQVTPTTAWNYGLVLDSS
                     NPAQSFTVTRQSGAVSANPFTQQTTPITMQVQAQQIADWQADDQNVVTPLQSSPVLAS
                     LPARTVTLIPMGAARLRISCFPTIGSGSGAHAWSWTGVYFRLQNQNSGKVLGVSDMST
                     ANSAQVVQYDDNGTADHLWALLDNGDGYFRIQNKNSGKVLGVDGMATANSANVVQFDD
                     NGTADHLWRWIDNGDGYVRIQNQNSGKVLGVSNMSTANSVNVVQYDDNGTTDHLWCFI
                     PDGPVRIQNQNSGKVLGVDGMSTANSANVVQFDDNGTTDHLWTFVPDASGYFRLRNQH
                     SGKVLGVSNMSTANSVNVVQYDDNGTADHLWRLRAGGGTYFRLQNKNSGKVLGVSNMS
                     TANSAQVVQFDDNGTADHLWRFA"
BASE COUNT          279 a          484 c          545 g          353 t
ORIGIN      
        1 tgcgcggctc ctgccgttcc ctccagccct gatctccggc cagacgatga tgaccagctc
       61 ccggacaaca ggttgcttgc gcccggcgct acgcccggct tgcatggcac ctggagcggg
      121 acaccgacca ctttcccctg aatgtacggc ggactgagcc gctcacgccc catcgcctgc
      181 caggaagaaa gaggcgatgg ggcttttcta gctcgtcctt ctacacaacg gcgccgggtt
      241 caggcgaaac gccagagatg gtcggcggtg ccgttgtcat cgaattgcac aacctgcgcg
      301 ctgttagccg tcgacatgtt gctgaccccc aggactttgc cgctgttttt gttctggagg
      361 cggaagtagg taccgccgcc ggcgcgcagt cgccagaggt gatcggcggt gccgttgtcg
      421 tcatactgca cgacgttgac gctgttggcc gtggacatgt tgctgactcc cagcaccttg
      481 ccgctgtgct gattgcgaag acggaagtag ccgctggcgt cgggcacgaa ggtccagaga
      541 tggtccgttg tgccgttgtc gtcgaactgc acgacgttgg cgctgttggc cgtcgacatg
      601 ccgtcaaccc ccaggacttt gccgctgttc tgattctgaa tgcgcaccgg gccatcggga
      661 atgaagcacc agagatggtc cgtggtaccg ttatcgtcat actgcacgac attgacgctg
      721 ttagccgtgg acatattgct gactcccagg actttgccgc tgttctggtt ctggatgcgc
      781 acatacccat cgccgttatc gatccagcgc cagagatgat cggcggtgcc attatcgtcg
      841 aattgcacga cgttggcact gttggccgtc gccatgccat caacccccag gactttgccg
      901 ctgtttttgt tctggatgcg gaagtagccg tccccattgt ccagcagcgc ccagagatgg
      961 tcggcggtgc cgttgtcgtc gtattgcacc acctgcgcgc tattggccgt ggacatatcg
     1021 ctgacgccca gcaccttgcc gctgttctgg ttctggaggc ggaagtagac gcctgtccag
     1081 gaccaggcgt gcgctcctga gccgctgccg atggtcggga aacaggagat gcgcaggcgg
     1141 gcggctccca tggggatgag cgtgactgtc cgggcaggca gactggcgag tacggggctg
     1201 ctctgcagag gggtgacgac gttctgatcg tcggcctgcc agtcagcgat ctgctgcgcc
     1261 tgaacctgca tagtaatggg ggtcgtctgc tgggtgaaag gattggcgga aacggccccg
     1321 gactgacgcg tgacggtgaa cgactgcgcc gggttgctgc tatcgagcac cagcccgtag
     1381 ttccaggccg tggtgggcgt gacctgatat tcgggaacct gggcagtgct attgttgatg
     1441 agggtgtaat cttcgccgat ctgtaaggca aaggtcagcg gaccgtagtt gaccgagacg
     1501 gcgttatggt tgttcgccca ggtctgcaca ctggtctgca tgggcagttg cagggtgacg
     1561 gtatcgccgc tggcccaggt ccgattgatg gtcacgaagg cggggccggc gctggcggag
     1621 acgctctgac cgttgacgcg cagcgctggc cgggtacacc a
//
LOCUS       DASZYN010000762         6816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_177890, whole genome shotgun sequence.
ACCESSION   DASZYN010000762 DASZYN010000000
VERSION     DASZYN010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177890"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="VHE33_19460"
     CDS             complement(<1..1092)
                     /locus_tag="VHE33_19460"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HVT99687.1"
                     /translation="MSDEPELQYQVTHARPQAFYTNFERKDEMQHQTHYCPGCGHGTI
                     HKMLAQAIDELGIQNRTILVSPVGCSVFAYYYFDVGNIQSAHGRAPAVATAAKRSRPE
                     SIVVSYQGDGDLSAIGAAEILHAANRGENISVIFVNNAIYSMTGGQVAPTTLLGQRST
                     TTPGGRNPANEGYPLHVSELLATLEAPVYIERVGLGDHKQIAQAYRAVRKALENQVKG
                     QGFSLVEVLSPCPTIWKMSPVDAQHWVRDVMEKTFPLGATRDRTSESPKMPVPSAPVP
                     VTEIPAILGTNDPSAESQTALGKVPGITNLQVRIAGFGGQGVLLLGEVLAEAGLDAGL
                     EVSWLPSYGPEMRSGTSNCHVRLSTEPIDC"
     gene            complement(1085..2164)
                     /gene="vorB"
                     /locus_tag="VHE33_19465"
     CDS             complement(1085..2164)
                     /gene="vorB"
                     /locus_tag="VHE33_19465"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HVT99688.1"
                     /translation="MSRQLIKGNEAIVKAAVLAGCRGFYGYPITPASEITEAAAEYLP
                     RVGGVFLQAESEVAAINMLYGAASAGVRVMTASSGPGISLMQEGISYMAGAELPCVIA
                     DITRAGPGLGNIASEQSDYHQVVKGGGHGSYRTLVLAPHSVQEMADLTTLAFELADRY
                     RNPVVLMADGFIGQMMEPVEFPDTAIEPSLPNWAVAGTEETRGNLISSIYLDSDKLEA
                     HVRQLEAKYCMAQRLETRAEELYTDDADVVLVGYGIVARILKAVAAEARAAGYKVGVL
                     RPVTLFPFPSASFQRIARRAKVFVVVEMSLGQLLDDVRLALNGTRPVEFLGRVGGNIP
                     SHDDVLAFVQEQARRCAESKQVAHV"
     gene            complement(2161..2427)
                     /locus_tag="VHE33_19470"
     CDS             complement(2161..2427)
                     /locus_tag="VHE33_19470"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HVT99689.1"
                     /translation="MAEAEKRDRGSLLIDTDECKGCGLCIEACPPKVIHLSHRLNPYG
                     YRTAVYTGAGCTGCGMCFLVCPEPGAVTVQRLARTTKPKTAVGQ"
     gene            complement(2384..3739)
                     /locus_tag="VHE33_19475"
     CDS             complement(2384..3739)
                     /locus_tag="VHE33_19475"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HVT99690.1"
                     /translation="MTPSQALGAVRSGARVWIQSGVGTPSTLVNALVARAGELRDVEI
                     VHMMTLGSADYTRPEYRGVFRHRGLFLGANVREAVCAGRADYTPIFLSEIEGLFVNGQ
                     LPLDVVLMQVSPPDQHGFVSLGTTVDCTLTASRCASIVIAEVNEQMPRTHGDTFLHVS
                     HIHAFVETSRPLLELESEAFTDVHLQVARNVASLIPDGATLQTGIGGIPEAVLTCLED
                     KRDLGIHTEMFSDSVIELMEAGIINGQRKSLHRGKAVAAFVLGTRRLFDFIHENPAFE
                     FRSISYTNDPFVVAQNDRMVAINSALQVDLTGQVCADSLGTKPYSGFGGQVDFIRGAA
                     RSKGGVPIIALLSTARAGQLSRIVPVLEPGAGVVTSRADVHYVVTEHGIAYLHGKTLR
                     ERAEALIAIADPRFQAQLEDFAVRAHYLERKPAPALVEQVWEGASNGRGRKARSRIAA
                     D"
     gene            complement(4141..5067)
                     /locus_tag="VHE33_19480"
     CDS             complement(4141..5067)
                     /locus_tag="VHE33_19480"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HVT99691.1"
                     /translation="MKIRLSRRSFIRGSAVLAGAQAAGLTRLWGADAQSKPGLQMYMV
                     AAEYRKDPAGTLAKLRAIGYEYMEAYSALITNIPAFKKRLVTAGLGCPSAHFAFGFVE
                     TEKVLDDAGALGVHYAVSSMLPPHPPKDGDTKATLQMMNHLTADDFRRMASIANQIGA
                     AAQKRGMEYAYHNHNFEFRKLNGDETGYDIFLKETDPKLVKLEVDAGWMAAGGANPAA
                     VIAANAQRVRLLHFKDFSTITPPVNELGEAAGSHIVDLGKGVAPLKDAYEAARNGGAK
                     YFIVDHDPPFHGQTVFEAAKIDYDYVAKLMRV"
     gene            5312..6184
                     /locus_tag="VHE33_19485"
     CDS             5312..6184
                     /locus_tag="VHE33_19485"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HVT99692.1"
                     /translation="MRFKSCLVTALSVVFFAAVAGAQSTSSAAQMPPPPHAKQIHLKH
                     VLVIGQTKGFEHDSISDAMAAIYNMGKDSGLWDTMMRTDTELLTKKDLGRNTKNLNYF
                     DLIVFASTTGELDLDDSQKQDILSFVHDDGKGFVGIHAALDTNYKWPEYGEMIGGWFD
                     QHPWFTFNAPIINEDPDFPAVRHFPKSFVKYDEIYQPKDWSRDKVNVLLSLDPTKLNY
                     ENNPRIHRTDHDFAVAWSKMYGKGRVFYSTLGHTEESWDDPDIRKMYFEAIKWALWMT
                     NGSTASHPRPAPPQ"
     gene            6319..>6816
                     /locus_tag="VHE33_19490"
     CDS             6319..>6816
                     /locus_tag="VHE33_19490"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HVT99693.1"
                     /translation="MKPPVSIRINRRDFLASAGMLIADQSVGRKLHAQARPAGKADHT
                     LRIEPCSLEIGQGVTVKTIAYNGQVPGPILHLREGVPVTIDVTNASANADIVHWHGLA
                     IDSLNDGAMEEGSPMIAAGETHRYTFTPKPAGTRWYHTHAGAGGDLGKGTYTGQFGFL
                     LIEGSQ"
BASE COUNT         1326 a         2110 c         2052 g         1328 t
ORIGIN      
        1 acagtcgatg ggttcggtcg acagccgcac gtggcaattg gaggttccgg agcgcatctc
       61 gggaccgtag gaaggcagcc aggaaacctc cagtcccgca tcgagtccgg cctcggccag
      121 cacttcgccc aacagcagaa ctccctggcc gccgaagccg gcgatccgca cctggaggtt
      181 ggtaatccca ggcactttgc ccagcgcggt ttgtgattcc gcggaaggat cgttcgttcc
      241 cagaatcgca gggatctcag tgacgggaac gggagccgag gggaccggca tttttggcga
      301 ttcgctggtg cggtcgcgcg tcgcgcccag cgggaaggtt ttctccatga cgtcgcgcac
      361 ccagtgctgc gcgtccaccg gcgacatctt ccagatggtc gggcacggcg agagcacttc
      421 gaccagagaa aacccctggc ccttgacctg gttctccaga gccttgcgca ccgcgcgata
      481 ggcttgcgcg atttgtttgt gatcgccaag gccgacgcgc tcgatgtaga ccggagcctc
      541 gagcgtcgcc agcagctcgc tgacatggag cgggtagccc tcgttggcgg ggttgcggcc
      601 gccgggggtc gtggtgcttc gctgtcccag gagagtagtg ggcgccacct ggccgccggt
      661 catgctgtag atggcgttgt taacaaagat gacgctgata ttctccccgc gattggcggc
      721 gtgcaggatt tcggcagcac caatggcaga caaatcgcca tcgccctggt agctcaccac
      781 gatgctttcc ggacggctgc gtttggcggc ggtcgccacg gccggcgcgc ggccatgcgc
      841 cgactgaata ttcccgacgt cgaagtagta gtaggcgaag accgagcagc cgacgggact
      901 gaccagaatg gtgcggttct gaatgcccag ctcatcgatc gcctgggcga gcattttgtg
      961 gatcgtgccg tgcccgcaac ctggacaata gtgggtctgg tgctgcatct cgtccttgcg
     1021 ctcgaaattc gtatagaagg cctgaggacg agcatgggta acctggtatt gcaactccgg
     1081 ttcgtcagac atgggccacc tgcttgcttt ccgcacagcg tcttgcctgt tcctgaacga
     1141 aggccagcac atcatcgtgg gacgggatat tgcctccgac gcggcccagg aactccaccg
     1201 gccgtgttcc gttcagcgcc agccgcacat cgtcgagcag ctggccgagg ctcatctcca
     1261 ccaccacgaa gaccttcgct cgcctcgcga tccgctggaa gcttgctgac ggaaacggga
     1321 acagcgtgac ggggcgcagg acgccaactt tgtatccggc ggcgcgcgcc tcggcggcta
     1381 cggccttcag aatgcgcgcc acaatcccgt accccaccag caccacgtct gcatcgtcgg
     1441 tgtacagctc ttcggctcgg gtctcgagcc gttgcgccat gcagtacttg gcttccagct
     1501 ggcggacatg cgcttccagc ttgtcgctgt ccaggtagat cgagctgatg aggttgccgc
     1561 gcgtctcttc cgttcccgcc accgcccagt tcggcagact gggttcgatc gcggtgtcgg
     1621 gaaactcgac cggctccatc atctgcccga tgaagccgtc ggccatcagc accacgggat
     1681 tgcgatagcg atcggcaagt tcgaaggcca gcgtggtcag atcggccatc tcctgcaccg
     1741 aatgtggagc cagcaccagg gttcggtagc tgccgtggcc gccgcctttg accacctggt
     1801 ggtagtcgct ctgctcggac gcgatattcc ccagccccgg acccgcgcgg gtgatgtcgg
     1861 cgatcacaca gggcagctct gcgcccgcca tgtagctgat gccttcctgc atgaggctga
     1921 ttcccggacc gctggatgct gtcatgacgc gcacaccagc cgaggctgcg ccgtacaaca
     1981 tattgatggc cgctacttcg ctctcggcct ggagaaagac gccgccgacg cgcgggagat
     2041 attccgcggc tgcttcagta atctcgctgg ccggtgtaat cggatatccg tagaagccgc
     2101 ggcagcctgc gaggactgcg gcttttacga tcgcctcgtt ccctttgatc agctgccggc
     2161 tcattggccc accgccgttt tgggctttgt cgtccgcgcc aggcgctgaa cggtgacggc
     2221 tcccggctcc ggacaaacca ggaaacacat gccacaaccg gtgcagccgg ccccggtata
     2281 gacggcagtc cggtatccat agggattcag ccgatgactc aggtggatca ccttgggcgg
     2341 acaggcttcg atgcacaacc cgcagccttt gcactcgtcg gtgtcaatca gcagcgatcc
     2401 gcgatcgcgc ttttcggcct cggccattgg acgctccttc ccaaacctgt tcgaccagcg
     2461 cgggcgctgg tttgcgttcc agatagtgcg ctcgcaccgc aaagtcctcg agctgggcct
     2521 ggaagcgcgg atcggcaatc gcaatcagcg cttctgcccg ctcgcgcaga gtcttgccat
     2581 ggagataggc gatgccgtgc tcggtgacca cataatgcac gtcggcccgc gacgttacga
     2641 cgcctgcacc gggttcgagc acgggcacaa tgcgcgaaag ctggcctgcg cgcgccgtcg
     2701 acagcagagc gatgatcggc actccgccct tcgaacgcgc ggcaccgcga atgaaatcca
     2761 cctggccgcc gaagccgctg tagggtttcg ttcccaggga gtcggcgcac acctgcccgg
     2821 tcaaatccac ctggagcgcg gagttgatgg ccaccatgcg atcgttctgg gccaccacga
     2881 agggatcgtt ggtataggag atggaccgga actcgaaagc cgggttttcg tgaatgaagt
     2941 cgaagagccg cctggttccc agcacgaacg ccgccactgc ttttccgcgg tgcagcgact
     3001 tcctctgccc gttaatgatt ccggcttcca tcagctcgat cacgctgtcg gagaacatct
     3061 ccgtgtggat tcccaagtct cgcttgtcct cgaggcaggt cagcacggcc tccggaatgc
     3121 cgccgatccc cgtctgcaac gtggcgccgt cggggatgag cgaggcaaca ttgcgcgcaa
     3181 cctggagatg gacatcggtg aacgcctcgc tctccagctc gagcagcggc cgcgaggtct
     3241 caacgaacgc atggatatgg ctgacatgga ggaacgtgtc gccgtgggtg cggggcatct
     3301 gctcgttcac ttcggcgatg acgatgctcg cacagcggga agccgtcagc gtgcaatcca
     3361 ccgtcgtgcc caggctgacg aatccatgct ggtccggggg cgacacctgc atcagcacca
     3421 cgtcgagggg caactgcccg ttcacaaaca acccttcaat ctcgctcaga aagatcgggg
     3481 tgtagtcggc gcgcccggcg cacacagcct cacggacatt cgccccgaga aataatccgc
     3541 gatgccggaa cactcctctg tactccggcc tggtgtagtc ggcgctgccc agggtcatca
     3601 tgtggacgat ctccacatcg cgtaactcgc cggctcgagc caccaaagca ttcacgagcg
     3661 tcgaaggcgt gcccacaccc gactggatcc ataccctggc gccggaacga actgcgccaa
     3721 gagcctgcga cggcgtcata cgcctgcttc ggaaatcgtc tttccaggtc atgtttcctg
     3781 actccgggtg caccttaagg catgcattcg gctgtgctta ctgcaatctg ggctgcgatc
     3841 cggggacgaa ccatggcccc cacagccgcc cgcctgcgga ccctctgcgg ggtctctgct
     3901 ccaaaaccta cgtacgttac gctccgcacg aaggcgttgg cagtgacccg tatcacggct
     3961 gtcaggcgtg tgattctgag gcaggggatg gagagatggg gtggcgaaga ggagggccca
     4021 agaagtgcgg agaacccgtg tcctgtcagc cgatcccggt agcaggaccg ttagcgtgca
     4081 agcttcactg acaggaaacc gggacgtcgg gactcagaag cgccgcggaa cttcgggcgg
     4141 tcagactctc atcagctttg ccacgtagtc atagtcgatc ttcgccgcct cgaatactgt
     4201 ctgtccgtgg aacggcggat cgtgatcgac gatgaaatac tttgcgccac cattccgggc
     4261 agcctcatag gcgtctttta gaggagcgac tccctttccc agatccacga tatgcgaccc
     4321 ggccgcttct cccagttcgt tcacaggagg cgtgatcgtg ctgaagtcct tgaagtgcag
     4381 caatcgcacc cgctgagcat tcgcggcaat gaccgcggcg ggattggcgc cccccgctgc
     4441 catccatcca gcgtctactt ccagcttgac cagcttcggg tcggtttcct tgagaaagat
     4501 gtcgtacccg gtttcgtcgc cgttgagttt acgaaattcg aaattgtggt tgtggtaggc
     4561 atactccatt ccgcgtttct gcgcggccgc tccgatctga ttggcgatcg atgccatccg
     4621 gcgaaagtcg tcggcggtca gatgattcat catttgcagg gtcgccttcg tatcaccgtc
     4681 cttcggcggg tgaggcggca gcatcgaact taccgcgtag tgcacgccca gcgcgcccgc
     4741 gtcgtccagg accttttccg tttccacaaa cccgaacgca aaatgggccg atgggcagcc
     4801 gagtcccgca gtgacaagcc tctttttgaa agcgggaata ttcgtgatga gcgccgagta
     4861 agcttccatg tactcatagc cgatcgccct cagcttcgcc agggtgccgg cgggatcttt
     4921 cctgtattcg gcggccacca tgtacatctg caggcctggc ttcgattggg cgtccgcgcc
     4981 ccacagacgc gtgaggccgg ccgcctgcgc acctgcaagc acagcggaac cgcgaatgaa
     5041 actacggcgg gacaggcgaa tcttcatttt cagctccttg atatggctca aacggtgcgg
     5101 gcacgcggtt ccgagcgctc ggatcgggcg ggcggtccgg ataagaatag ccgagccagg
     5161 gacaaacttc gctggttgct atggcgggcg agtgtttttt gtacgaaccg gaagtcgcgg
     5221 tcgagggata tcgaaaacct ttacaatggg atgcttcaga agcatcgtcc ccgatacggt
     5281 gcgaaaattc tgagagttca gaggaggctc attgcggttc aaatcttgtt tggtgacggc
     5341 tctttccgtc gttttttttg cggccgttgc gggcgcacag agcacaagca gcgctgcgca
     5401 gatgccgccg cctcctcatg caaagcaaat ccacctgaag catgttctgg tgatcgggca
     5461 aacgaaggga ttcgagcacg attccatttc ggatgcgatg gccgcgatct acaacatggg
     5521 caaggacagc ggcctgtggg acacgatgat gcggaccgac accgagctgc tgacgaagaa
     5581 ggatctgggc cgcaacacaa aaaatctgaa ctacttcgat ctgattgtct tcgccagcac
     5641 cacgggcgag ctcgatctgg acgacagcca gaagcaggac attctttcgt tcgtccacga
     5701 tgatggcaag ggattcgtag gcatccacgc cgcgcttgac acgaattaca agtggccgga
     5761 atacggggag atgatcggcg gctggttcga tcagcatccc tggttcacct tcaacgcgcc
     5821 catcattaat gaagatccgg attttcccgc cgtgcgtcac tttcccaaat cgttcgtgaa
     5881 gtacgacgag atctaccagc cgaaagactg gtcacgcgac aaagtgaacg tgctgctgag
     5941 ccttgacccc acgaagctga actacgagaa caatccgcgc attcatcgca ccgatcacga
     6001 ttttgccgtc gcctggagca agatgtacgg caaagggcga gtgttctatt cgaccctcgg
     6061 gcatacagag gaatcgtggg acgatcctga cattcgcaag atgtacttcg aagccatcaa
     6121 gtgggcgctt tggatgacga atggaagcac ggcgagccat ccgcgcccgg cgccaccgca
     6181 atagctcacg ttttcttcat cggccggatg cggcgtgagc tgtttccgga atgaagacgt
     6241 ttacacgcac gacctcgtcc gacgaaaata atccgtgccc atttgcaagt atccccatct
     6301 cgctgcgaga agaatgcatt gaaaccaccc gtgtcgattc gtatcaaccg cagagacttt
     6361 ctggcttcgg ccggaatgct gatcgccgat cagtctgttg gccgaaaact gcatgcccag
     6421 gcccgcccgg ccgggaaggc cgatcacact ttgcggatcg agccctgcag tcttgagatc
     6481 ggccaaggcg tcacggtcaa aaccatcgcc tacaacggac aggtaccggg tccgatcctg
     6541 catcttcgcg aaggggtgcc ggtcacaatc gacgtcacca acgccagcgc gaatgctgac
     6601 atcgttcact ggcatgggct ggcgatcgat tcgctcaacg atggcgcgat ggaagagggg
     6661 tcgccaatga tcgccgccgg cgagacccat cgctacacgt tcacgccgaa accggcgggc
     6721 acgcgctggt atcacaccca cgccggcgcg ggcggcgatc tgggcaaagg cacctacacg
     6781 ggacaattcg gcttcctgct catcgagggc agccag
//
LOCUS       DASZYN010000763         3106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1018818, whole genome shotgun sequence.
ACCESSION   DASZYN010000763 DASZYN010000000
VERSION     DASZYN010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018818"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(634..>3106)
                     /locus_tag="VHE33_19495"
     CDS             complement(634..>3106)
                     /locus_tag="VHE33_19495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT99694.1"
                     /translation="IAQNLRFDLRFALRVFRRNPAFSVAAVLSVAIGVGATVAVFSVV
                     NALLLKPLPYQDPDRLVILWNTSPGLNITQDWFSTAQYFDIKNGHHGLEQVALAIGGN
                     ENLTGTGDPERVGTIHVTANLLAMLGVQPHLGRLLQPWEDDPGHAATAVLSYGMWTRR
                     FNADPRVLGKSITLNGKNYEVVGVLPRSFTLPREVLPTLGGAEQSDILIPLVLGPGSA
                     TMRDHEDYNIIGKLRPGVSIAQARAEMDTITATLRRDHPDLYPPNGGLTFAIVPLLEQ
                     VVGDTRRPLMILLGAAGFVLLIACANLANLWLSRALARRQEIALRTALGANRARVVRQ
                     LITECALLGVIGGALGTLFAASAVAVFRSLRPVNVPRVQDIAIDGRVLAFSLLLSLLA
                     GLLFGIVPALRSSRVDLVSALKGGSRTASETGSLWGRGGKLRKLLLVSEVALAVVLLV
                     GAGLLTRSFAALLVVNPGFNADRVLTLGLTMTGRQYTDRQHVVATYRQLWDRLDHIPG
                     VTASGGITSLPLSEMYAWGPITVEGQPLPPGENFINADQRIVAGQYFTAMQIPLLRGR
                     LFTENDNAATPRVVIIDDSMAAQFWPHQDPLGKRIRLGSLTSNAPVLTVVGVVGRIKQ
                     YTLDSDSRIALYIPHLQYPTRDLNIVVRSAGAPSSIAGAVTRAVHDLDPNLPVYAVRS
                     MQQRVADSLARRRFAMGLLAFFAVVSLTLAALGTFAVIATLVNQGLPEIGIRIALGAT
                     RLHILNMIMIRAMLLAACGVASGLVTAAILSRFMRSLLFGVLPHDPATYLVVSAVLVG
                     VSLVATGIPAGRAARTDPMTSLRSE"
BASE COUNT          624 a          808 c         1183 g          491 t
ORIGIN      
        1 cattgcagca ttcgtggttg tgagtgcggc attcgcgatc gtcagcgctg cgctcgcggt
       61 tgtgaatgca gcactggcat ttgtgaggga agcaccggga atccggcggg cgcatggcgc
      121 aggcatctga tcggaaccga tcggaagcag gaagaagaac cggtgacggc gactgggttg
      181 ggcatcgagt tcctcctccg gcggggttta ggcaaagcaa aaggctcgcc gcagcgagcc
      241 ttttcagggt tcttctcccc tatttttagg atatcaagcg gagcgagaaa tcgtgccaac
      301 gtttttggag gcttagccgg gataagtcac gggaaatgag ggagaacact ttcgtaccgt
      361 tcgagaaaag cttgacaaga atccacaggt ggcgggctcg gctccgatga gccaaagcgg
      421 atcccacggg gagcgaaacg agtgaagccg gtgaaccgtc gtggcgcaac acggcgtatt
      481 gctcccgcag gctcagcgat cacaatgaag aggccgcgtc tcgcttgcgc cgctggcgca
      541 tcgaacggtt cgcggttttg agagaggccc cgtcccagtg gcggggcttt tgttgcgccg
      601 ttggcgtcgc caggagaaag gcaaccgcgc gttctattcg ctgcgcaggc tggtcatggg
      661 atcggtcctg gctgccctgc cggcaggaat gcccgtcgcc accagcgaaa cacccaccag
      721 taccgccgac accaccagat aagtggccgg atcatgcggc aggaccccaa acagaaggct
      781 gcgcatgaaa cgactcagga tcgctgcagt gaccaggccc gaggctactc cacaagcggc
      841 gagcagcata gcccggatca tgatcatgtt cagaatgtgc aggcgggtgg cgccgagcgc
      901 gatgcgaatc ccgatctccg gcaggccctg gttgaccagg gtggcgatga cggcaaaggt
      961 gccgagggcc gcgagggtaa gcgaaaccac ggcaaagaag gccagcaggc ccatggcaaa
     1021 gcgacgtcgg gcgagggagt cggcgacgcg ctgctgcatg gagcgaacgg cgtagacggg
     1081 gaggttggga tcgagatcgt gcacggcacg cgttacggca ccggcgatgc tggacggggc
     1141 gccggcactg cgcacaacga tgttgagatc gcgggttgga tattgcagat gcgggatgta
     1201 gagcgcgatg cgagagtccg agtccagcgt gtattgcttg atgcggccaa cgacgccgac
     1261 gacggtgagc acgggcgcat ttgacgtgag ggagccgaga cgaatcctct tgccgagcgg
     1321 gtcctgatgc ggccagaact gcgcagccat ggagtcgtcg atgatgacga cgcgcggagt
     1381 ggcggcgttg tcgttttctg tgaagagacg gccgcgcaga agcgggatct gcatggcggt
     1441 gaaatattga ccggcgacga tacgctgatc ggcgttgatg aagttttcgc cgggaggcag
     1501 cggctggcct tcgacggtga tgggtcccca ggcgtacatc tcacttaaag gcagcgaggt
     1561 gatgccgccg gaagccgtga cgccggggat gtgatcgagg cgatcccaga gctggcgata
     1621 ggttgcgacg acgtgctggc gatcggtgta ctggcgtccc gtcatggtga ggccgagggt
     1681 gaggacgcgg tcggcgttga atccgggatt gacgacgagg agcgcagcga agctgcgggt
     1741 gaggaggccg gcgccgacca gcagaacgac ggcgagagcg acttcggaga cgagaaggag
     1801 cttgcggagt ttgccgccgc ggccccagag cgatccggtc tccgatgcgg ttcgactgcc
     1861 gcctttgagc gcggagacga ggtcgacgcg ggaggagcga agagcgggaa cgatgccgaa
     1921 gaggagtccg gcgagtagcg acagcaacaa ggagaaggcg agcacgcgac cgtcgatcgc
     1981 gatgtcctgc acgcggggca cgttgacggg gcgcagggat cggaagacgg cgacggcgga
     2041 ggcagcgaac agggttccga gcgcgcctcc gatgacgccg agcagggcac attcggtgat
     2101 gagctggcgg acgacgcggg ctcgattggc gccgagagcg gtgcggaggg cgatttcctg
     2161 gcggcgcgcg agggcgcggg agagccagag gttggcgaga ttcgcgcagg caatgagcag
     2221 gacaaatccc gctgcgccga gcaggatcat gagcgggcgc cgcgtgtcgc cgacgacctg
     2281 ctcgagcagg gggacgatgg cgaaggtgag tccgccgtta ggcggataga ggtcgggatg
     2341 atcgcggcgg agcgtggcgg tgatggtgtc catttcggcg cgggcctggg cgatggagac
     2401 gccgggccgg agcttgccga tgatgttgta atcctcgtga tcgcgcatgg tggcggagcc
     2461 gggaccgagg acgaggggaa tgaggatgtc ggactgctcg gcgccgccga gggtgggaag
     2521 aacttcgcga ggcagcgtga agctgcgcgg cagaactccg acgacttcgt agtttttgcc
     2581 gttgagggtg atcgatttac caaggacgcg cgggtccgcg ttgaagcggc gggtccacat
     2641 gccatagctc agcaccgcgg tggcggcgtg accgggatcg tcttcccagg gctggagaag
     2701 ccggccgagg tgaggctgga cgccgagcat cgcaagaaga ttcgcggtga cgtggatggt
     2761 gccaacgcgc tcgggatcgc cggtgccggt gaggttttcg ttgccgccga tggcgagagc
     2821 cacctgctcc aggccgtggt ggccgttctt gatgtcgaag tactgcgctg tggagaacca
     2881 gtcctgggtg atgttgaggc cgggcgaggt attccagagg atgacgaggc gatcgggatc
     2941 ctggtaaggg agaggcttga gcagcagcgc gttgacgacg ctgaagacgg cgacggtggc
     3001 tccaacgccg atggcaacag agagaacggc ggccacggag aaagccggat tcctgcggaa
     3061 gacacgcagg gcaaagcgga gatcgaaacg gagattctgg gcgatt
//
LOCUS       DASZYN010000764         2930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1076664, whole genome shotgun sequence.
ACCESSION   DASZYN010000764 DASZYN010000000
VERSION     DASZYN010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076664"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VHE33_19500"
     CDS             <1..621
                     /locus_tag="VHE33_19500"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HVT99695.1"
                     /translation="LVAVVGSGIMAERLSAGNMGIALLANAIATGAALVVLITVLGPL
                     SGAHFNPAVSLFFALRGAMPGREALLYGGVQVAAAVLGVWLAHAMFAMPLLQISTHLR
                     DGLAQVLAEFIATFGLLLTIAGALRFVPASAPMLVGLYITGAYWFTASTSFANPAVTV
                     ARMLTDSFSGIAPSSAPGFIAAQLLAVFCAWPLLGWLLGSKGGMEV"
     gene            complement(675..2480)
                     /locus_tag="VHE33_19505"
     CDS             complement(675..2480)
                     /locus_tag="VHE33_19505"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVT99696.1"
                     /translation="MLKSNCHFRVSITVTVLCVAVVVAFVKPGAAQQSISPDTAPPAA
                     VAENHDVSRLSALPETITAHAITSDVDVTDSQMLNANRDKDNWLLYGRTYDNQRFSPL
                     TQISKSNVKKLRPVAIIQTGIANSTETNPIVVNGIMYISTPSDHVQAYDAVTGRMLWS
                     YTPADMETLNLCCGPQSRGVAVAYGKVFLAQLDAKMVALDARSGKLVWKSDLSSTMPP
                     DSRHFSYTSAPLVYDGMVVIGSAGAEFPMRGFVQAYDANTGEIIWRFRTTAAPDEPGG
                     DSWSGDSWKTGGGSVWNTPAYDPKNGLISFAVGNPNPDVDGDDRLGANAYTDSMVALH
                     SRDGKLAWWNQQVPHDQWDLDATGPVIFFDAKDENGKSVPAAGEAGKEGHVFIVNRLT
                     GELLRKSQPFVLESPNKFTPPTEAAGEPHYPSVAGGGQWSPSAFSPLTHDFYVMGTNA
                     AYSYQLVKDPPGAPDSGIRFGGKVRNISEGSIESSGTLTAIDVNSGNIAWQYKSGFPM
                     LGGVLATASNLVFAGEMDGNFNAFDAQSGRKLWHFNLGTGVCAPPVTYHVNGVQYVAV
                     AAGGNGANGMQRLTASRGLVPFGDVVAIFALPDQE"
BASE COUNT          567 a          891 c          795 g          677 t
ORIGIN      
        1 ctggtcgcgg tggtcggctc gggcatcatg gccgagcgcc tgtcggcggg gaacatgggg
       61 atagcgctgc tggccaacgc catcgccacc ggtgcggcgc tggtggtgct gatcaccgtg
      121 ctggggccgc tgtccggggc gcatttcaat cccgccgtgt cgctgttctt tgccctgcgc
      181 ggcgccatgc cagggcgcga ggcgctgctc tatggcggcg tgcaggtggc ggcggcggtg
      241 ctgggcgtgt ggctggccca tgccatgttc gccatgccgc tgctgcaaat ctccacccat
      301 ctccgggacg gcctggcgca ggtcctggcc gagttcatcg ccaccttcgg gcttttgctg
      361 accatcgcgg gcgcgctgcg ctttgtgccc gcatcggcgc ccatgctggt cgggctctac
      421 atcaccggcg cctattggtt caccgcctcg acctccttcg ccaatcccgc cgtcaccgtg
      481 gcgcggatgc tgaccgacag cttttcgggc atcgcgccat cctcggcgcc cggcttcatc
      541 gcggcccaac tgctggcggt gttctgcgcc tggccgctgc tgggctggct gctgggcagc
      601 aagggcggga tggaggttta gcggcgcggc gcccgatctc ggcgaacgca gcgagcttga
      661 aaaatcataa gtcttcattc ttgatctgga agcgcgaaga tcgcgaccac gtctccaaaa
      721 ggaacaagtc cacgagacgc agtcagtctt tgcattccgt tggcgccgtt gccaccggcg
      781 gccactgcca catattgcac cccgttcaca tggtaagtga ctggaggcgc acaaacaccg
      841 gtcccaagat taaaatgcca gagttttcgg ccgctctgag cgtcaaaggc gttgaaattg
      901 ccgtccattt ccccggcaaa caccaaattg ctcgcggtgg ccaagacacc gcccagcatc
      961 ggaaatcccg atttgtattg ccaagcgata ttgccgctat tgacatcaat agccgtcaga
     1021 gtccccgaag actcaattga gccttcagaa atatttcgta ctttcccacc gaatctaatg
     1081 ccgctatccg gggcgccagg cggatccttc acgagctgat agctataagc tgcattcgtg
     1141 cccatgacat agaaatcatg cgtcaatggt gaaaatgcag atggactcca ctgcccaccg
     1201 ccagccaccg aaggataatg cggctctccc gccgcttccg ttggtggtgt gaatttgttt
     1261 gggctttcca aaacaaaggg ctgagacttc cgaagcagct cgcccgtcag acgattgaca
     1321 ataaaaacat gcccttcttt tcccgcctct cccgctgcgg gtacactttt cccgttctcg
     1381 tccttggcgt cgaaaaatat gacaggccca gtcgcatcca aatcccactg atcgtgcggg
     1441 acctgctgat tccaccatgc gagcttgcca tccctggagt gaagcgcaac catcgaatct
     1501 gtgtaggcgt tcgcccccag tcgatcatcc ccgtcaacat ccgggttcgg attgcccact
     1561 gcaaaactta tcaagccatt ttttggatca tatgctggcg tattccaaac cgatccacct
     1621 cccgttttcc aggaatctcc actccagcta tcgcctcccg gctcgtccgg tgctgccgtt
     1681 gtgcgaaaac gccagattat ttctcctgta tttgcatcat atgcctgtac gaagccgcgc
     1741 atcggaaatt ccgcccccgc gctccctatg acaaccatgc catcgtaaac aagaggcgct
     1801 gacgtgtatg aaaaatgccg tgagtcaggc ggcatggtac tgctcaaatc gctcttccaa
     1861 acgagtttcc cgctgcgggc atcaagtgcc accattttgg catccagttg agccaaaaag
     1921 acctttccat aggcgacggc gacaccacgg gactgcggcc cacaacatag gttgagcgtt
     1981 tccatatcag cgggggtgta tgaccacaac atacgtcccg tgacggcgtc atatgcctgt
     2041 acatgatcgc ttggcgtcga gatgtacata atgccattga cgacaatagg atttgtttca
     2101 gttgaattcg ctatcccggt ttggataatc gcaacgggtc gaagcttttt tacatttgat
     2161 ttcgatatct gggtaagcgg tgaaaagcgc tgattgtcat atgtgcggcc ataaagcagc
     2221 cagttgtcct tgtcgcgatt ggcattgagc atttgtgaat ccgtcacgtc cacatcggac
     2281 gtaatcgcgt gcgcggtgat ggtttcaggc agagcgctta gccgtgagac atcatgattc
     2341 tcggcaaccg ccgctggcgg ggccgtatct ggagagatgg attgctgggc cgcgcccggc
     2401 ttcacaaagg ccacgacaac agccacgcag agcaccgtga cggtaatgct gacgcgaaaa
     2461 tggcaattcg attttaacat ggcgtttccc tgggcggttt tctgatgcct aacttgatct
     2521 agactatttg aatcgacctg gcggtttctt tacaccaccg gctgtccgtc gtaaaaacat
     2581 tggggatgcg cttcgcgtga cgcgcctggt cgggccgcaa aattcctcgc gccaggcctc
     2641 gccagcgcgc tctctgcatt ttctcgagaa gcacgtttcc tcgggggagc aacgctctgt
     2701 tcgccaaatg ccactatacc ggccaccatc ggctgatgga actttcccgc cccctccaca
     2761 ttacctcccc gatagtttga gaggtattcc atgtcattgc tcctgatcat ccttcttgtg
     2821 gttcttctgg ccggcggcgg ctttggctat agccgctacg gcatgggcgg cggctccggc
     2881 attgtcggca ccatcctgat catcatcctg gttctctatc tgctgggcat
//
LOCUS       DASZYN010000765         1719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1019139, whole genome shotgun sequence.
ACCESSION   DASZYN010000765 DASZYN010000000
VERSION     DASZYN010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019139"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VHE33_19510"
     CDS             complement(<1..419)
                     /locus_tag="VHE33_19510"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HVT99697.1"
                     /translation="MISDLWYKNAIFYCLSVATYMDANGDGVGDFPGLLRRLDYLQGM
                     GVTALWLMPFQCSPGRDNGYDVSDYYAVDPRYGTMGDFVEFTRGCKQRGMRLIVDLVV
                     NHTSNEHPWFRDACSNPQSKYRDWYVWSNSRPKDANKG"
     gene            464..>1719
                     /locus_tag="VHE33_19515"
     CDS             464..>1719
                     /locus_tag="VHE33_19515"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HVT99698.1"
                     /translation="MQDLSWWKHAVIYQIYPRSFQDSNGDGIGDLNGIRSRLDYVVDL
                     GVDAVWISPIYPSPMADFGYDVADYCGIDPIFGTMQDFDELLADLHRRGLRLILDFVP
                     NHTSDQHPWFIESRSSRENPKRDWYLWRDQPNNWQSHFGGSAWEYDASTQQYYLHEFL
                     KEQPDLNWRNPSVKAAMFDALRFWLRKGVDGFRVDVMWLMIKDAQFRDNPPNPAYEQG
                     QGTRNRFLPVYDADRPEVHELVEEMRGILDDFGDRVLIGEIYLPVQRLMQYYGPNLQG
                     ANLPFNFQLLQCAWTAEDLARTIGDYVSALPPGAWPNWVLGNHDQPRIASRIGLRKAR
                     AAALLLFALPGTLTVYYGEELGMQNVPIPPDEVQDPAEKRQPGIGMGRDPERTPMPWD
                     GSLSSGFSTGRPWLPIGDANQALNVQS"
BASE COUNT          359 a          520 c          499 g          341 t
ORIGIN      
        1 cccttatttg catccttcgg cctgctgttg gaccacacat accagtcgcg atatttcgat
       61 tgcggattcg agcaggcatc ccggaaccag ggatgctcat tcgaagtgtg gttcaccacc
      121 agatcgacga tcaggcgcat cccgcgttgc ttgcagccgc gggtaaactc aacgaagtcg
      181 cccatcgttc cgtaacgagg atccacggcg tagtagtcgc tgacgtcgta gccattatcg
      241 cggccgggcg aacactggaa cggcatgagc cacagcgcag taacgcccat cccctgcagg
      301 taatcgagcc ggcggagcag gcccgggaag tctcccacgc cgtctccgtt cgcatccata
      361 tatgtagcca cggaaagaca gtaaaagatt gcgttcttat accagagatc gctgatcata
      421 agggcttgcc tttgcaaaac agtaccaggg agcacaggtt gggatgcagg atctgagttg
      481 gtggaagcac gcggtcattt atcagattta tccgcgatca tttcaggaca gcaacggaga
      541 tgggatcggg gatctgaacg gcattcgcag ccgcctcgat tacgtggtcg atctcggagt
      601 ggacgccgtc tggatctccc ctatctatcc atcgcccatg gccgattttg gatacgatgt
      661 cgccgactat tgcggcatcg accccatctt cggcacgatg caggatttcg atgagctgct
      721 ggcagacctg catcggcgcg gccttcgcct catcctggat ttcgttccca accacacctc
      781 cgaccagcat ccctggttta tcgaaagccg ctcgtcgcgc gagaatccga agcgggactg
      841 gtatttgtgg cgcgaccagc ccaacaactg gcagagccac ttcggcggat ccgcctggga
      901 atacgacgcc agcacgcagc agtactacct gcacgaattc ctgaaagaac agcccgacct
      961 gaactggcgc aacccgtcgg tgaaggccgc aatgttcgac gcccttcgct tctggctgcg
     1021 caaaggcgtg gatggcttcc gcgtcgacgt catgtggctg atgatcaaag acgcccagtt
     1081 ccgggacaac ccgccgaatc ccgcttacga gcaggggcag ggtacccgca acaggtttct
     1141 gcccgtctat gacgcggatc gccccgaggt ccacgagttg gtcgaggaaa tgcgcgggat
     1201 tctcgatgat tttggcgatc gggtgctcat cggagaaatt tatctgccgg tccagcgcct
     1261 aatgcagtac tacgggccga acctccaagg cgccaatctg cctttcaact tccagcttct
     1321 gcaatgcgcc tggaccgccg aggatctggc gcgcacgatt ggcgattacg tgtccgcgct
     1381 cccgccgggc gcgtggccga actgggtgct cgggaatcat gaccagccac ggatcgcatc
     1441 cagaatcggc ctcagaaaag ctcgtgccgc tgcgctgctg ctgttcgcac ttcccgggac
     1501 gctgacggtt tattacggag aagagctggg catgcagaat gtacccattc cacccgacga
     1561 ggtgcaggat ccggccgaga aaaggcaacc gggcattggg atgggccgcg atccggagcg
     1621 aacgccgatg ccctgggatg gttcgctctc atcgggattt tcaaccgggc gaccgtggct
     1681 accgatcggc gatgccaacc aggccctgaa cgtgcaatc
//
LOCUS       DASZYN010000766         7324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_375912, whole genome shotgun sequence.
ACCESSION   DASZYN010000766 DASZYN010000000
VERSION     DASZYN010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7324
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375912"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VHE33_19520"
     CDS             <1..307
                     /locus_tag="VHE33_19520"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVT99699.1"
                     /translation="GQAGQANYAASKAGLIGLTKSLARELASRNITANAIAPGYIETA
                     MTAVLDEKQRESMLAHIPLGRAGTDADIANAVRFLASDDAAYITGHVLDVNGGMYMG"
     gene            362..1954
                     /locus_tag="VHE33_19525"
     CDS             362..1954
                     /locus_tag="VHE33_19525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HVT99700.1"
                     /translation="MKILTADEMRNVDRLTTERYGVASLDLMHEAGSAVARFVMREFR
                     ESRHIAVLCGKGNNGGDGFVAARELDAAGRKVRILLLGFPADLKGDAKAAFEELTLAP
                     VLAADEAALDSPDVQSLLASADLFLDAVVGTGFKPPLRDVAAALRDRVRALNTPVVAV
                     DLPSGWDADSRSFTAEGAFPAHAVVTFTAPKLAHVSGNLTGSSTGPIVVAPIGSPEDA
                     IRSGTNLTWAGASKSIAEQPRTPDANKGLYGHVLVAAGGRGKSGAPAMASLAALRAGA
                     GLVTAAVPESILSSVAAIAPELMTLPLREGPHGEIDASNLEQLPRIIERATVLAMGPG
                     MGTDPEAFVLGMIEKTKLPLVVDADALNILAKRPDKIDGSKRTVVLTPHPGEMARLAG
                     ISTKEVQASREPLAREFATRHNVTVVLKGWRTLIAHPDGRIAINTTGNPGMAKGGSGD
                     ILTGIIAALLAQHPDHPAEAVEAAVYLHGLAADFALREQDEHTLLATDTIDHLFRAFL
                     FRSRDEAGYVWLEGLPSSAARA"
     gene            1954..2430
                     /gene="tsaE"
                     /locus_tag="VHE33_19530"
     CDS             1954..2430
                     /gene="tsaE"
                     /locus_tag="VHE33_19530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HVT99701.1"
                     /translation="MTQTADLKVHQFESHSGKETIEIGHRIAALLTPPKFLILRGDLG
                     AGKTTLVKGIAEALDAAEPDEVTSPTFTLIHEYEGTRSTNGHKEPVILYHLDLYRIQN
                     ERQLDSLGLDDLSSPDSMVLVEWGEKFPSVVKRSQGEIEMRTTRGDSRSITLTLRD"
     gene            complement(2461..3165)
                     /locus_tag="VHE33_19535"
     CDS             complement(2461..3165)
                     /locus_tag="VHE33_19535"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99702.1"
                     /translation="MNMWQQVEQSLHSSMGLVMNKIATLLPGILAFIVAFLFFLLLGW
                     LVSWIVIRILSGLRFDERIGRGANAMAEFSPRATPSLLIGRVVFWCFVIAGVVVGVSA
                     LGTEEHTISGWMLAYVPRVVGAAILLLAGNVLARFVSRSVLITGVNLNLSFARLLSMG
                     VRWLILVLTGAMVLDHLALGGEIVELGFGILFGGIVLALSLAVGLGSRDLVSRSLERE
                     ASRPTEVVPPEKVHHF"
     gene            complement(3201..4442)
                     /locus_tag="VHE33_19540"
     CDS             complement(3201..4442)
                     /locus_tag="VHE33_19540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99703.1"
                     /translation="MAAVELTEETKQQVERIGSADVVVGIAGAVSPEELVPRAERILQ
                     EMGSAVSSLRFVFAWPGRMPENSRPASAENAPLTLLPFPPPPPAEFWAGVSANQRAVL
                     GLAASLNARVCIVLGSDLAALDARAIQLLAYGILERQCGLVMPIYPTAKYDGLINSGV
                     LAPLSRALYGRRVRFPLVFDFAVSGALCASLSRDTPAERGGNGLLWPVTVASTQHAQL
                     AVGQVHIDVHHKPATAGLELSAVLGQLAGSLFQEMDLCASHWQRVRGSLPTPIWGSGG
                     EDLPDGQPVDPQPMLDTFQLGSRNLDEVWRLILPPNSLLELRRLARLAPDQFQMPDAL
                     WASIVYDFGLAWRLRTISRGHLLGALTPIYLGWAASYVREVSSLNHAAAEQRLEQLAR
                     VWEEKKPYLLSRWRWPDRFHP"
     gene            complement(4460..>7324)
                     /gene="treS"
                     /locus_tag="VHE33_19545"
     CDS             complement(4460..>7324)
                     /gene="treS"
                     /locus_tag="VHE33_19545"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HVT99704.1"
                     /translation="DQKYQDARIIFTDTEKSNWTWDPVAQAYYWHRFFSHQPDLNYDN
                     PEVLNEVLSVMRFWLDMGVDGLRIDAIPYLVERDGTNCENLPETHAVIKAIRAAIDES
                     YAGRMVLAEANQWPTDVRPYFGDGDECHMAFHFPLMPRIYMALRQEDRLPITDIMAQT
                     PAIPDNCQWGLFLRNHDELTLEMVSDDERDYMYLAYSADPRMRINIGIRRRLAPLLDN
                     NRRRIELLNSILFSFPGTPILYYGDEIGMGDNIYLGDRNGVRTPMQWSPDRNAGFSRA
                     NPAKLYSPVIMDPVWGYEAINVEAQQNDPSSLLNWMRNMIALRKLFQVFGRGSLRFLD
                     ASNRKILAYIRQYDGEMVLCVANLSRFAQPVQLDLSGMEGMTPVEMLGYVEFPTITKT
                     PYSLTLGPYGFLWLEIQPSMASAESASAVGDEQSFYVEHERDWQGILSGDGVRRLEAM
                     LPPWLQKQRWFGSKSRTIVRARIVDSLQFNDHRSALVWICVDYVEGEPDSWLLCMALS
                     FGDDARTLQESTPQSIVASVGSATRSGVLHEGMVANAPEALLDIVAGGAELHTRNGTL
                     VGLPGSVLAGLRGAEPLPVRLSSAEQSNTSAIFDDRLIMKLFRRQQPGRNPDVEIGRF
                     LTEEAKFANIPPFGGSLEVRRPNEEEPSTVVLLQGLVQNEGDGWQWTLDELDRYYEST
                     ASLRTPVSPAVGPESSDPHPDARHHAGFYLDAAAVLGRRTAEMHLALASGHDAAFSPH
                     NPEARELEDLRDHLAGHGAHAFDLLKTHLSRLPEDTIEQAGLALSRRGVLLTKFRSLP
                     ALAVGQWTRIHGDYHLGQVLRSKGDFVILDFEGEPARPLAERRAKQSPLKDVAGMLRS
                     FSYAAFSALTRYTTRRPEDFAQLESWARLWEHSVSSEFLRAWRSTVAGSPVVPAGEKD
                     FEALLQIYMLDKALYELVYELNNRPTWIRIPLHGILSLPL"
BASE COUNT         1488 a         2231 c         2320 g         1285 t
ORIGIN      
        1 gggccaggcg ggtcaggcga actacgccgc cagcaaggcg gggttgatcg gactgaccaa
       61 atcgctggcg cgggagctgg cttcgcgcaa catcaccgcc aacgccattg ctcccggcta
      121 catcgaaacc gctatgaccg ccgttctgga cgagaagcag cgcgaatcga tgctggcgca
      181 cattccgctg gggcgagcgg gcaccgatgc ggatatcgcg aacgcggttc gctttcttgc
      241 ctccgatgac gcggcctaca ttaccggcca tgttctggat gtcaacggcg gcatgtacat
      301 gggctgatgc aagaatgaaa catcgaaagc cgggcccgag cttcgacgac cacaatctgc
      361 catgaagatt ttgaccgcag acgaaatgcg caatgtcgac cgcctcacga ccgagcggta
      421 cggggtcgca tccctcgacc tgatgcacga ggccggatcg gcggttgcgc gtttcgtcat
      481 gcgcgaattc agggagtctc gccacatcgc tgttttgtgc ggcaaaggca acaatggcgg
      541 cgatggattt gtggccgcgc gcgagttgga cgccgccgga cgcaaggtcc ggattctgct
      601 gcttggtttt cccgccgatt tgaaaggtga cgcgaaagcc gccttcgaag aactgactct
      661 ggccccggtg ctggcggcgg atgaagctgc gctcgattct cctgatgtgc aatcgctgct
      721 cgcgtcggca gacctgtttc tggacgctgt tgtcggcact ggcttcaaac cgccgctgcg
      781 tgatgtcgca gccgcattac gcgaccgcgt gcgcgctctc aacacgccgg tggtcgcggt
      841 tgacttgccg tcagggtggg atgccgattc gcgttccttc acggccgagg gtgcgtttcc
      901 tgcccatgcc gtcgttacgt tcaccgcacc caagctggcc cacgtttccg gcaaccttac
      961 cgggagttcc acgggaccga tcgttgtggc tccaatcgga tcgccggagg acgcgatacg
     1021 atctggcacg aatctcacct gggcgggagc gtcgaagagc atcgccgagc aaccgcgaac
     1081 gccggacgcc aacaagggac tttacggcca tgtactggtc gccgccggcg gacgaggcaa
     1141 atccggcgcg cccgccatgg cgtcactcgc agccctgcgc gcgggggcgg gcctggttac
     1201 agctgcggtg ccggaatcga tattgtcgtc cgttgcggca atcgcaccgg agctgatgac
     1261 ccttcctctg cgagaaggcc cgcacgggga gattgacgcg tcaaatctgg agcagcttcc
     1321 gcgcatcatc gaacgcgcga ccgtgcttgc gatgggcccc ggcatgggaa cggaccccga
     1381 ggcttttgtg ctggggatga tcgagaagac gaagctgccg ctcgtcgtcg acgctgacgc
     1441 cctcaacatt ctggccaaac gcccggacaa gatcgacggc agcaaacgca ccgtggttct
     1501 cacgccccat cccggggaga tggcgcgcct ggccggcatc agcacgaaag aagtgcaggc
     1561 cagccgtgaa ccgctggcga gggagttcgc gacgcgccac aatgtgacag tggtgttaaa
     1621 aggctggcgc acgctgatcg cgcatcccga cggacggatc gccatcaaca ccaccggaaa
     1681 tccggggatg gccaaaggcg gcagcgggga catcctgacc ggcatcatcg cggccctgct
     1741 ggcgcagcat cccgatcatc cggccgaggc ggtggaggcc gcggtttatc ttcacggtct
     1801 tgcagccgac ttcgccctgc gggagcagga cgagcacacg ctgctggcga ccgacacaat
     1861 tgatcatctt tttcgcgctt ttctctttcg ctcgcgggac gaagcgggat atgtttggtt
     1921 ggagggactt ccttcctccg cggcgagggc gtgatgacac agactgctga tctgaaggtg
     1981 catcagttcg agagccacag cggcaaggag accatcgaga tcggtcatcg tattgccgca
     2041 ttgctgacac cgccgaagtt tctcatcctg cgtggggatc tgggcgcggg caaaacgaca
     2101 ctggtgaaag gtatcgccga agcgctggat gccgcggagc cggatgaggt gaccagcccg
     2161 accttcacgc tgatccacga atatgaaggc actcggtcga caaacggcca caaggagccg
     2221 gtgatccttt accacctcga tctgtatcgg atccagaatg agcggcagct tgattcgctg
     2281 ggactggacg acctgagcag tccggacagc atggttctgg tggagtgggg agagaaattt
     2341 cccagcgttg tgaaacgcag ccagggcgaa atcgagatgc gcacgacgcg tggagactcc
     2401 cggagtatca cgctgacgct gcgcgactga aacacgccct ccgggctcgc gacaattcac
     2461 ttagaagtga tggacctttt ccgggggcac gacttcggtc gggcgcgatg cctcgcgctc
     2521 cagggaccgg ctcaccaggt cgcgtgatcc cagtcccacc gcgagagaaa gagccagtac
     2581 gattccgccg aagaggatgc cgaatcccag ctcaacgatc tcgcctccca gagccaggtg
     2641 gtcgagcacc atggcgccgg tcaggaccag gatcagccac ctcacgccca tactcagcag
     2701 ccgggcaaag ctcaggttga gattgacacc tgtgatgagc acgctgcgcg agacgaagcg
     2761 cgccaggaca ttcccagcca gcagcaggat tgccgctccc accacgcgag gaacataggc
     2821 cagcatccac ccggagatgg tgtgctcctc ggtgcccagt gcagacacgc caacgaccac
     2881 gccggcgatg acgaagcacc agaaaacgac ccggccaatg agcagggagg gtgtggcgcg
     2941 cggactgaac tcggccatgg cattggcgcc gcggccgatg cgctcatcga agcgcagccc
     3001 ggagaggatg cggatgacga tccacgaaac gagccatccg aggaggagga agaacaggaa
     3061 ggcaacgatg aaggcaagaa tgccagggag cagcgtggcg attttgttca tcaccagccc
     3121 catcgacgag tgaagcgact gttccacctg ttgccacata ttcatttctc ctttcgatcg
     3181 ccgcctgtag aaacagagac tcagggatgg aagcgatcgg gccatctcca ccgtgagagc
     3241 agatacggct tcttttcctc ccacacgcga gccagctgct ccagcctttg ctccgcagcg
     3301 gcatgattca ggctggaaac ctcgcgaacg taagaggcgg cccagccgag atagatcggc
     3361 gtcagcgcgc ccagcaggtg cccacggctg atcgtgcgca gacgccaggc gagcccaaaa
     3421 tcgtacacga tgctggccca aagcgcgtcc ggcatctgaa actgatccgg tgcgagccgc
     3481 gcgagccggc gcagttccag cagactgttc ggcggcagaa tgaggcgcca cacttcatcg
     3541 aggtttctcg atccgagctg gaatgtatcc agcatgggtt gaggatcgac cggctggccg
     3601 tcgggcagat cttcgccccc gctgccccag attggcgtcg gcagagatcc gcgaacccgc
     3661 tgccagtgcg aagcacacag gtccatctcc tgaaagagcg agccagccag ttggcccagc
     3721 accgcgctca actccagccc ggcggtcgct ggcttatggt ggacgtcgat atggacctgt
     3781 ccgacggcca actgagcatg ctgggtcgaa gctaccgtca cgggccagag taatccgttg
     3841 cctcccctct ctgccggggt gtcccgagag agcgaagcgc acaaggctcc cgaaaccgcg
     3901 aaatcgaaaa ccagggggaa gcggactcgg cgtccataga gagcacgact gaggggtgcg
     3961 agcacaccgc tgttgatgag gccgtcgtac ttggcggtgg gataaatcgg catgacgagg
     4021 ccacattggc gttcgaggat gccataggca agcagctgga tggcgcgagc gtcgagcgct
     4081 gcgagatccg accccagcac gatgcagacc ctcgcattca gtgaggcggc caggcccagc
     4141 acggcacgct gattcgccga gacgcccgcc cagaactcgg cgggcggtgg aggcggaaac
     4201 ggcagcagcg tcagcggggc attctccgcg gaggcagggc gggagttttc cggcatcctc
     4261 ccgggccatg cgaagacgaa gcgaagcgag gagacggcag agcccatttc ctgcaggatt
     4321 cgctccgccc gcggaaccag ctcttccggt gaaacggcgc cggcgattcc aacgaccaca
     4381 tcggccgagc cgatccgctc gacctgctgt ttcgtctcct cggttaattc aacagctgcc
     4441 attcagggcg atccttatgt cacagcggca gtgacaaaat accgtggagc ggaatgcgaa
     4501 tccacgtcgg gcggttgttc agttcgtata ccagttcgta cagcgcttta tcgagcatat
     4561 agatttgcag cagcgcctcg aaatcctttt cgccggcagg aacaacagga gagccggcca
     4621 ccgttgagcg ccaggcgcgc agaaattcgc tggagaccga atgctcccag agccgcgccc
     4681 atgactccag ctgcgcgaag tcttcaggcc ggcgggtggt gtagcgagtg agagcagaaa
     4741 aagctgcata gctgaaggaa cgcagcatcc cggccacgtc tttaagaggc gattgcttcg
     4801 cgcgccgttc ggcaagcggg cgcgcgggct ctccttcaaa atccagaatg acgaagtcgc
     4861 ctttggaacg aaggacctgg ccgagatggt agtcgccatg aattcgggtc cattgtccga
     4921 ccgcgagcgc aggcagggac cggaacttcg tcagcaatac accgcggcgg ctgagggcga
     4981 ggcccgcctg ttcgatcgtg tcttccggca accgggagag atgcgtcttg agcagatcga
     5041 aggcgtgtgc gccgtgaccc gcgaggtgat cccggagatc ttcaagttcg cgtgcttcgg
     5101 gattgtgcgg agaaaatgcc gcatcatggc ccgaggccag ggccagatgc atctccgcgg
     5161 tgcgccggcc cagtaccgcc gccgcatcaa gataaaagcc ggcatggtga cgggcatcag
     5221 gatgcggatc ggaggattcc gggccgaccg ccggggagac cggagttctt aaagaagccg
     5281 tgctttcgta ataacgatcg agttcgtcca gggtccactg ccagccatcg ccttcgttct
     5341 ggaccaggcc ctgcagcagc acgacggttg acggctcttc ctcgttcggc cgccgcactt
     5401 ccagagatcc gccgaacggc gggatattcg cgaatttcgc ctcttcggta aggaatcgac
     5461 cgatttcgac gtcgggattg cggcccggct gctggcgccg gaagagcttc atgatcagcc
     5521 gatcatcaaa aatcgcagag gtattactct gttcggcgct gctcaggcgc acagggagcg
     5581 gctcggcgcc acgcaatcca gcgagtacgg atcccggcag gcccacgagc gtgccattgc
     5641 gcgtgtgcag ttctgcgccg ccggcgacaa tatcgagcag agcctcgggg gcattcgcca
     5701 ccataccttc atgcaggacc ccgctgcggg ttgccgaacc cacactggcc acaatcgact
     5761 gcggcgtcga ctcctgcaac gttctggcgt cgtctccgaa gctgagcgcc atgcacaaga
     5821 gccagctgtc cggctcccct tcgacatagt cgacgcaaat ccacaccagc gccgaacggt
     5881 ggtcgttgaa ctgtagcgag tcaacaatgc gggcgcggac gatcgtcctc gatttgcttc
     5941 cgaaccagcg ttgtttctgg agccagggcg gcagcatcgc ttccagccgc cgcacaccat
     6001 caccggacag gatgccttgc cagtcgcgtt catgctcgac atagaaactc tgttcgtcgc
     6061 cgaccgcgga tgcgctttcg gcggaagcca tcgacggctg aatctccagc cacagaaatc
     6121 cgtacgggcc cagcgtgagg gagtagggcg ttttggtaat ggtcgggaac tcgacgtagc
     6181 ccagcatctc aaccggcgtc atgccctcca tgccggacag atccagctgc acgggctgtg
     6241 cgaaacgcga caggttcgcg acgcagagca ccatctcgcc gtcgtactga cgaatgtagg
     6301 cgagaatttt gcggttcgac gcatcaagaa agcgcagcga cccgcgcccg aagacctgga
     6361 acaacttgcg cagcgcgatc atgttgcgca tccagttcag caacgacgat ggatcgttct
     6421 gctgcgcctc gacattgatg gcttcgtagc cccagaccgg atccataatg accgggctgt
     6481 agagcttcgc ggggttcgcg cgcgagaagc cggcgttgcg atcgggcgac cattgcatcg
     6541 gtgtgcgcac accattgcgg tcgccgagat agatgttgtc gcccatgccg atctcgtcgc
     6601 cgtagtacag gatgggcgta ccggggaagg agaagaggat gctgttgagg agttcgatac
     6661 gacgccggtt attgtcgagc agcggagcca ggcggcgccg gatgccgatg ttgattcgca
     6721 tccgcggatc ggcgctgtaa gccaggtaca tgtaatcgcg ctcgtcgtcg ctgaccattt
     6781 ccagcgtcag ctcgtcgtga ttgcgcagga acagccccca ctggcagtta tccggaatgg
     6841 ccggggtctg ggccatgatg tccgtgatgg gcagccgatc ctcctgacgc agcgccatgt
     6901 agatgcgcgg catcaagggg aagtggaagg ccatgtggca ctcgtcgcca tcgccgaagt
     6961 acggccgcac gtccgtgggc cactgattgg cttcggcgag caccatgcgt cccgcatagc
     7021 tttcgtcgat tgcggcacga atggccttga tcaccgcatg ggtttcgggg aggttctcac
     7081 agttggtgcc gtcccgctcg acaagatagg gaatcgcgtc gatgcgcagc ccgtcgactc
     7141 ccatgtcgag ccagaagcgc atcacgctca ggacttcgtt cagaacttcg gggttgtcgt
     7201 agttcaggtc aggctggtgc gagaagaagc gatgccaata gtaagcctgc gcgacaggat
     7261 cccaggtcca gttcgacttc tcggtatcgg taaagatgat gcgcgcatcc tgatacttct
     7321 gatc
//
LOCUS       DASZYN010000767         3592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_426449, whole genome shotgun sequence.
ACCESSION   DASZYN010000767 DASZYN010000000
VERSION     DASZYN010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426449"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /gene="trmFO"
                     /locus_tag="VHE33_19550"
     CDS             <1..645
                     /gene="trmFO"
                     /locus_tag="VHE33_19550"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HVT99705.1"
                     /translation="ARRGRDTLRFGPMKPVGLRDPRTGKTPWAVVQLRVENLRADSYN
                     LVGFQNHMKFGDQARVFRMIPGLEQAKFLRYGQIHRNTYINSPTVLTRTLHLREYPRI
                     FFAGQISGVEGYTESIATGMLAGIYAAAMARGERPEPVPRGTALGSLVRYITEADAKR
                     FQPANITFDLLEPLDEATRRKVRDKRERHRMVCERALEAMEAWLRPASVAGNRE"
     gene            695..1015
                     /locus_tag="VHE33_19555"
     CDS             695..1015
                     /locus_tag="VHE33_19555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99706.1"
                     /translation="MVLWLRSKATVSEARGVILAEVREGRGTDKPSAYADFDTEVGIG
                     RISVWVSGEVDFEVLRRSDARMVYFGHVTTSTLTGSALDRALDAFLTTMTNPKDDLLT
                     LNEL"
     gene            1012..1710
                     /gene="purQ"
                     /locus_tag="VHE33_19560"
     CDS             1012..1710
                     /gene="purQ"
                     /locus_tag="VHE33_19560"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HVT99707.1"
                     /translation="MKFGVLVFPGSNCDHDTYNVVAEIAHQPVTFLWHDSPSLENCDA
                     ILVPGGFAYGDYLRTGAIARFSPVMQSVKKFADDGGLVLGICNGFQILCEAGLLPGAL
                     MRNAGLRYICKQVYLRTETTETPFTQTLTKGQVLKIPIGHMEGNYFCDEATLAALKSQ
                     DRIVFRYSTPEGAITPEANPNGSLENIAGILNEGRNVLGMMPHPDRSSESILGSSDGL
                     KIFDSLVGALARSA"
     gene            1707..2498
                     /locus_tag="VHE33_19565"
     CDS             1707..2498
                     /locus_tag="VHE33_19565"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HVT99708.1"
                     /translation="MIDIHHHLLFGTDDGPKDIDSSVAQARAAAADGITHIACTPHAN
                     DTWDFSPERNHEKLDAIRERVGDAVTLGLGCDFHLSYDNIEDALKHPTKFTINDGKYL
                     LVEFAEFMIPQSIGDTFYEFSVKGMRPIITHPERNPILQKDHARMAPWIRTGCLVQIT
                     ASSLHGRFGKRAQSLAWELLEKNWVHFIATDAHNLEGRPPSMRPAYEAVAKRYGEGTA
                     ERLCVKNPRSAFENQPMGPQPEPEGIYVYEDEVKRPGLMGRLFGK"
     gene            complement(2444..2806)
                     /locus_tag="VHE33_19570"
     CDS             complement(2444..2806)
                     /locus_tag="VHE33_19570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99709.1"
                     /translation="MEIWAFANETEIGRAARAKRTDSFLMREVSTFWTFVEKRDLNVR
                     HQRSKVNGIFGNLAARDGKIHAGMTTDAVGALGMAQQGYDRLSERIESWRRKQIAATE
                     CYLPNKRPISPGRFTSSS"
     gene            2811..>3592
                     /locus_tag="VHE33_19575"
     CDS             2811..>3592
                     /locus_tag="VHE33_19575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99710.1"
                     /translation="MPRQPAAVPGGYFSSLNSTPQHRAENRLSAAANVATQAQGQNVV
                     SVPTFSSSFTSDGDSYAYTMVGQNPALRRTTSVPTAYVPLSFYFDEFVDQNGNNITID
                     ATTITREVKNSPLFDDAEYATGNTQFVDAQMRAQFWPLINTRRGDNYHVLLERPQTLT
                     PVTIEVPFGASQVFADGSGNYFALIDVNFIESQLSTMLQLEPVDVRSIPIFLTRNAVY
                     GDFSMGQPVDCCVGGFHTAYVARQTANRIFVQTVVFATSLDSA"
BASE COUNT          724 a          959 c         1187 g          722 t
ORIGIN      
        1 gcgcgtcgcg ggcgggacac gctgcggttt gggcccatga agccggtagg tctgcgcgat
       61 ccgcggacgg ggaagacgcc gtgggcggtg gtgcagttgc gggtggagaa cctgcgcgca
      121 gattcgtaca acctggtggg cttccagaac cacatgaagt ttggcgacca ggcacgggtc
      181 ttccggatga ttccggggct ggagcaggcg aagtttttgc ggtacgggca gattcaccgg
      241 aacacctaca tcaactcgcc gacggtgttg acgcgtacgc tgcatctgcg cgagtatccg
      301 cggatcttct ttgccgggca gatatcggga gtggaagggt acacggagtc gatcgcgaca
      361 ggaatgctgg cgggtatcta tgcagccgcg atggctcggg gtgagaggcc ggagccggtg
      421 ccgcgcggaa cggcattagg gtcgctcgtg cgctatatca cggaggcgga cgcgaagcga
      481 ttccagcctg cgaatattac gtttgacttg ctggaacctc tggacgaagc gacgcgcagg
      541 aaggtgcggg acaagcggga gcggcaccgg atggtgtgtg agcgcgcgct ggaggcgatg
      601 gaagcatggc tgcgacctgc ttccgtcgca gggaacaggg agtaggcaat agagggccag
      661 atcaatgacc aattacccgc ttactgtgga ggaaatggtt ttatggcttc gttccaaagc
      721 gaccgtttcg gaggctcgtg gcgtgatcct tgccgaagtc cgcgaggggc ggggtaccga
      781 taagccttcg gcgtacgcgg acttcgatac cgaagtcggg attgggcgga tcagcgtgtg
      841 ggtatccggt gaagtcgatt ttgaggtgct tcggcgatcg gatgccagga tggtctattt
      901 cggtcacgtg acgacctcca cgctgaccgg cagcgctctt gatcgagccc tcgatgcgtt
      961 cctgacgacg atgaccaatc ccaaagatga cctgctaacc ttaaatgagc tatgaaattt
     1021 ggtgtgctgg tgtttccggg atcgaattgc gaccacgata cttacaacgt ggtggccgag
     1081 attgcgcatc agccggtgac gtttttgtgg cacgactcac cgagcctgga aaactgcgac
     1141 gccattctgg tcccgggggg atttgcctac ggggattatc tgcggaccgg ggcgattgcg
     1201 cggttctcgc cggtgatgca gtcggtgaaa aaatttgccg acgatggcgg gctcgtgctg
     1261 ggaatttgca acggattcca gatcctgtgc gaggcaggat tgctgccagg cgcgctgatg
     1321 cggaatgcgg ggctgcgcta tatctgcaag caggtgtatc tgcggacgga gacgacggaa
     1381 acgccgttta cgcagacgct gacgaagggg caggtgctga agattcccat cgggcacatg
     1441 gaggggaatt atttctgcga tgaggcgacg ctggcggcat tgaagagcca ggaccgaatc
     1501 gtcttccgct attcgacgcc ggaaggggcg atcacgccgg aggcgaatcc gaacgggtcg
     1561 cttgaaaaca ttgcgggaat cctgaacgag gggcggaacg tactgggcat gatgccgcat
     1621 ccggaccgtt cgagcgaatc tattttggga tcgtcggacg gtttaaagat tttcgactcc
     1681 ctggtgggag ccctggcaag atcggcatga tcgacattca tcaccacctg ctgttcggga
     1741 ccgacgacgg gccgaaggat atcgacagtt ccgtggcgca ggccagggcg gcggcggcgg
     1801 acgggatcac gcacatcgcg tgcactccgc atgcgaacga cacgtgggac ttcagtccgg
     1861 agcggaatca cgaaaagctg gacgcaattc gcgagcgggt gggagacgcg gtgacgctgg
     1921 ggctgggctg cgactttcat ctttcctacg acaatatcga ggacgctctg aagcacccga
     1981 cgaagttcac gatcaacgat ggcaagtatc tgctggtgga atttgcggag ttcatgatcc
     2041 cgcagagcat cggcgatacg ttctacgagt tttcggtgaa agggatgcgg cccatcatta
     2101 cgcacccgga gcggaatccg attctgcaga aggaccacgc gcgcatggcg ccgtggattc
     2161 gcacgggatg tctggtgcag atcacggcgt cgtcattgca cgggcgcttc ggcaagcgcg
     2221 cgcaaagtct ggcctgggag ttgctggaaa agaactgggt gcacttcatc gcgacggatg
     2281 ctcacaatct cgagggacgg ccgccctcga tgcggcccgc ttatgaagcg gtcgccaagc
     2341 gatatggcga gggaacggcg gaacggctgt gcgtgaagaa tccgcgatcg gcattcgaga
     2401 atcagccgat gggaccgcag ccggagcccg agggtattta cgtctatgaa gacgaagtga
     2461 agcggccggg actgatggga cgcttgttcg gcaaatagca ttccgtcgcg gcgatttgct
     2521 tcctgcgcca actttcgatt ctttccgaca atctgtcgta accctgctga gccatgccga
     2581 gcgcgccgac cgcatctgtg gtcatccctg cgtgaatttt tccgtcgcga gcggccaaat
     2641 taccaaagat gccgttgact ttgcttcttt ggtgccgcac attgaggtcg cgcttctcaa
     2701 caaaagtcca gaaagtcgag acctccctca tgaggaagct gtcggttctt ttcgctcttg
     2761 ctgctctgcc gatctcggtt tcattcgcga acgcccaaat ttccacggcg gtgccacgtc
     2821 agccggccgc tgttccgggc ggttatttct cctctctcaa ctcgacgccg cagcaccggg
     2881 ccgagaaccg gctgtcggcg gcggcgaatg ttgcgacgca ggcgcagggg cagaatgtgg
     2941 tgtcggtgcc aacgttcagc agctcgttca ccagcgatgg cgacagctat gcgtacacca
     3001 tggtgggaca gaacccggca ctgcggcgga ccacaagcgt gccgacagcc tacgttccct
     3061 tgtcgttcta cttcgacgag tttgtggacc agaacggcaa caacatcacg atcgacgcga
     3121 ccacgattac gcgtgaggtc aagaattcgc cgctgttcga cgatgcggaa tacgccaccg
     3181 gcaacacgca gtttgtggat gcccaaatgc gtgcgcagtt ctggccgctg atcaacacgc
     3241 ggagaggcga taactaccat gttctgctgg aaaggccgca gacgctgacg ccggtaacga
     3301 tcgaagtgcc cttcggcgca tcgcaggtgt ttgcagacgg aagcgggaac tactttgcgc
     3361 tgatcgacgt gaatttcatc gaatcgcagc tgagcacgat gctgcagctg gagccggtgg
     3421 acgttcgctc gatcccgatc tttctaacgc gcaatgcagt ctacggcgac ttttcgatgg
     3481 ggcagccggt ggactgctgt gtgggcggat tccacacggc gtacgtagcg cggcagacag
     3541 cgaaccggat ctttgtgcag acggtggtct ttgcgacatc gctcgattcc gc
//
LOCUS       DASZYN010000768         1688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1268190, whole genome shotgun sequence.
ACCESSION   DASZYN010000768 DASZYN010000000
VERSION     DASZYN010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268190"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VHE33_19580"
     CDS             <1..259
                     /locus_tag="VHE33_19580"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HVT99711.1"
                     /translation="LKEKLLAAHRAGIFEAILPEDNRKDLPDIPDNLRTAMKLNFVDS
                     MDEVLKIALEAPLPELKEETPEVLESGVLNTVPPPEQRPHQ"
     gene            complement(327..1316)
                     /locus_tag="VHE33_19585"
     CDS             complement(327..1316)
                     /locus_tag="VHE33_19585"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HVT99712.1"
                     /translation="MRIATILVLLASALGSGAQTSPVHTWRYIRVGAPQDIEVHPRAG
                     YALMGGGTDLDEAFRWLCDRADGGDFLILRARGDDDYNPYVQGLCHLNSVATLILPDR
                     VSSDDPFVTKTIADAEAIFIAGGDQGRYINFWAGTPVEEGLRRAVKRGVPIGGTSAGL
                     AVLGQYIYSAQNDKPDDKDLSSEEALADPFYRQVVIAPDLLGIPILRGIITDTHFDTR
                     KREGRLLVFMARILESGKTKTIRAIGVDERNAVLVDPDGHAQVVGKKDGEADFYEASG
                     KPQQCESGKTLRIRSVSRRAVPSGDSFDLTHWVGRGVAATLSVENGKIVRADS"
     gene            complement(1313..>1688)
                     /locus_tag="VHE33_19590"
     CDS             complement(1313..>1688)
                     /locus_tag="VHE33_19590"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HVT99713.1"
                     /translation="GRAVAEYLSKHPKVSNVLYPGLTSHPQHELAKRQMSGFGSMIAF
                     EVGSRANADKLLRSVRVITNGESLGGVESLISHSATTTHASVPAEEQKRLGITEGLVR
                     ISVGIEDEADILGDLDQALAKL"
BASE COUNT          342 a          540 c          486 g          320 t
ORIGIN      
        1 actcaaggaa aagctgctgg cggcacaccg cgccggcatc ttcgaagcga tcctcccgga
       61 agacaatcgc aaggaccttc cggatattcc cgacaacctc aggaccgcca tgaagctgaa
      121 ctttgtggat tcgatggacg aggtcctgaa gatcgcgctg gaggcgccgc tgcccgaact
      181 gaaggaagag acgcctgagg ttctggagtc gggcgttctg aacactgtcc cgccgccgga
      241 gcagagacca catcagtagc ccgcttagca ttagtcgtca gaatcgggaa ggcaggttcg
      301 gaatcgagcc tgccttttgc tcttgctcat gaatcggctc tgacgatttt cccgttctca
      361 acagaaaggg tggcggcaac gcccctgccc acccagtggg taagatcgaa actgtcccca
      421 ctgggaacag ctctcctgct gacggatcga atcctgaggg tcttccccga ctcgcactgc
      481 tgcggcttac cagaggcctc gtagaaatcc gcctccccat ccttcttccc caccacctga
      541 gcgtggccgt cgggatccac cagcactgcg ttgcgctcgt caacgccgat ggcgcgaatg
      601 gttttcgtct tgccggattc aagaattcgc gccatgaaga ccaacagacg cccctcccgc
      661 ttgcgcgtat cgaagtgcgt atcggtgatg atgccgcgca ggatcgggat accgagcaga
      721 tccggcgcaa tcacaacctg gcgatagaag ggatctgcca gcgcctcttc ggagctgaga
      781 tccttgtcgt cgggcttgtc gttttgcgcc gagtagatgt actggccaag gaccgcgagc
      841 cccgcactcg tgcccccaat cggaacaccg cgcttcaccg cacggcgcaa cccctcttct
      901 accggcgttc cggcccagaa attgatatag cgcccctgat cgccgccggc gataaagatc
      961 gcctctgcat ccgcgatggt cttcgtcaca aaaggatcat ccgatgacac gcggtcggga
     1021 agaatgagtg tcgcgacgga gttgaggtga cagagcccct gaacgtaggg gttgtagtcg
     1081 tcatcgccac gtgcgcgcag gatcaggaag tcgcctccat cggctcggtc gcagagccag
     1141 cgaaaagcct catccaggtc cgtgccccct cccatcaggg cgtatcccgc acgcggatga
     1201 acttcgatgt cttgtggcgc gcctacgcgg atgtatcgcc aggtgtgaac cggagaggtt
     1261 tgggcgccgc tgccgagcgc tgaagccagc agcaccagta tcgtggcgat cctcatagtt
     1321 ttgccagcgc ctgatcgagg tcaccgagaa tatccgcctc gtcttcaatg cccaccgaaa
     1381 tccgcaccag gccctccgtg atcccaaggc gcttctgttc ctcagccggg acggaggcat
     1441 gcgtcgtggt cgccgaatgc gaaatcagcg attcgacgcc gccgagcgac tcgccgttcg
     1501 tgatgacgcg aaccgatcgc agcagcttgt ccgcgttggc acgcgacccg acctcaaagg
     1561 cgatcatgga tccgaacccg gacatctgcc tcttcgccag ctcatgctgg gggtgcgacg
     1621 tcaacccggg atagagaaca ttgctcacct tcggatgctt cgaaagatat tcggcgacag
     1681 cgcgcccg
//
LOCUS       DASZYN010000769         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_747980, whole genome shotgun sequence.
ACCESSION   DASZYN010000769 DASZYN010000000
VERSION     DASZYN010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747980"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1932)
                     /locus_tag="VHE33_19595"
     CDS             complement(64..1932)
                     /locus_tag="VHE33_19595"
                     /inference="COORDINATES: protein motif:HMM:NF025469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4277 domain-containing protein"
                     /protein_id="HVT99714.1"
                     /translation="MRRPLVELSARTIFLAIPGLLTLLLLFLCHCASTPWLFGYPIFP
                     GGVMAQAWHGPTLADDTRHLGPLALIAPLLQRLDVAAIIDRHLPPDPQLAFPHGQVLS
                     LLLAARLCRPTALLNVPAWAGDTGADLLWDIPADALNDDRLGRALDAFFTQRHSILTS
                     VAAQALHLADLPRERLHFDPTHLCFAGAYESSQPRPLAVPRPGATAADMQPAHITHGY
                     GDDAKLIQVGVTCVVDELGAVPLVSQCLDGNANGHTAIRWQCDWLLAEGLLRPGTLLV
                     SDRGTFSAEHVARLHRHGCPVLCSVPWEDYQDVYDRQADRLDWQRASYLSLEQQRRRQ
                     TNSSLPQEYYNLAVLRHSLADPQSGEAIACRVLFVYSSADEDICRQTRERDIVRLRQG
                     LEAIAATVARGHPQTTPASISGRVARLFGKRAAARFFHWEMRPLTAEEQALLPPPARG
                     CCRPRYRFTYGFDEAAAVAAARYDGLAALLTTAPLQQSGDLLFGQFKQQNYVELCHHQ
                     WKTPLAVRPVFLKSPRRVEALVCLMQVALTAYQLLERLYRQSVAADADRSERHRTSES
                     LLRLFRSYGLIERQTVMGRVVHATRLSSEQSRVLRQLRFPTPAQLLARALAPLPLG"
     gene            2010..>2411
                     /locus_tag="VHE33_19600"
     CDS             2010..>2411
                     /locus_tag="VHE33_19600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99715.1"
                     /translation="MGLERHGDGRLHALIADMKSTTEAKVEHRLQIAFYHLMLDRILG
                     DAGITGTTIQTGILFKPPADPTPEEAVEVIPPLREAADKWFGLKDVLLEVVDDPEAYL
                     RSVRDLVTGPESTALRVGQAAFTDVPFCLSFK"
BASE COUNT          450 a          743 c          836 g          382 t
ORIGIN      
        1 ggctagaccc attttccgca ccctcttggc tgatgctccc tgtcctcgcg ttggcttgct
       61 gcgtcagccc agtggcagcg gagctaatgc ccgagccaac agttgcgccg gcgttgggaa
      121 gcgtagctga cgcaacaccc gcgactgttc cgatgacaga cgcgtcgcat gaaccacccg
      181 gcccattacc gtctgccgct cgatcaaccc gtagctgcgg aacagacgca acagcgattc
      241 gctcgtgcga tgccgctccg accgatcggc atcagccgct acactttgcc gatacagccg
      301 ctccaacagc tggtaggccg tcaacgccac ctgcatcagg cataccaacg cctccacgcg
      361 acgcggcgac ttgaggaaca ccgggcgaac cgccagcggc gtcttccact ggtggtgaca
      421 caactcgacg tagttctgct gcttgaactg gccaaacagc aggtcgccac tctgttgcaa
      481 gggagccgtc gtcagcaacg ccgccaggcc gtcataacga gccgccgcca cagccgccgc
      541 ctcatcgaag ccatacgtga agcggtagcg gggtcggcag caacctcggg ccggcggcgg
      601 caacagtgcc tgctcttcgg ccgtcagggg acgcatctcc caatggaaga agcgggccgc
      661 cgcccgcttg ccgaatagcc gggccacccg cccggaaatg gatgcgggag tggtctgcgg
      721 gtgaccacgc gccaccgtag ctgcgatcgc ttccagtccc tgtcgtaagc gaacaatgtc
      781 gcgttcgcgg gtctgccggc agatgtcctc gtcggcgctg ctgtagacga acaggacgcg
      841 gcaagcaatc gcttccccgc tctgaggatc ggccagcgaa tgccgaagta ccgccaggtt
      901 gtaatactcc tgcggcaacg acgagttggt ctgacgccgc cgttgctgct ccagcgacag
      961 gtagctggcc cgctgccagt cgagccggtc cgcctggcgg tcatacacgt cttgataatc
     1021 ctcccagggg accgagcaca gcaccgggca gccgtggcga tggaggcggg cgacgtgctc
     1081 ggcggagaag gtgcctcggt cggagaccag caacgtcccc ggccgcaaga gcccctcggc
     1141 aagcaaccag tcgcactgcc agcggatggc ggtgtggccg ttggcgttgc cgtccaggca
     1201 ctgactcacc agcgggacgg cacccagctc atcgaccacg caggtgacgc cgacctggat
     1261 gagcttggcg tcatcgccgt agccgtgcgt gatgtgagcg ggctgcatgt cggcggccgt
     1321 cgctcccggt cgggggacgg cgagcggccg tggttgcgac gactcgtaag ctccggcgaa
     1381 gcacagatgc gtggggtcga agtggaggcg ttcgcgtggc aggtcggcca ggtggagggc
     1441 ctgagcggcg accgaggtca ggatggaatg gcgttgcgtg aagaaggcgt cgagggctcg
     1501 accgaggcgg tcgtcattga gggcatcggc ggggatgtcc cagaggaggt cggctccggt
     1561 atcgccggcc caggcgggga cgttgagcag ggcggtgggc cggcagagtc gggcggcgag
     1621 caggaggctg aggacctggc cgtgggggaa ggccagttgc gggtccgggg gtaggtgtcg
     1681 gtcgatgatg gcggcgacat cgagccgctg caggagcggt gcgatgaggg ccaggggtcc
     1741 gaggtgacgg gtgtcgtcgg caagggtagg tccgtgccag gcttgtgcca tgacgcctcc
     1801 tgggaagatg gggtagccga acagccaggg ggtcgatgca cagtggcaca ggaataatag
     1861 caacagggtc aagagtccgg ggatagcgag aaaaatcgtg cgcgctgaca attcgaccag
     1921 cggtcggcgc atagaatcgg aaggcgagcg gacgcggaag tccgcaccct accagaatca
     1981 ggggaccatc atccaagagg gtgcggaaaa tggggctaga gcggcatggc gatgggcggc
     2041 tgcacgccct catcgcggac atgaaaagca cgaccgaagc gaaggtcgaa caccgcctcc
     2101 agatcgcgtt ctatcacttg atgctcgacc gcatactggg ggatgccggg attactggca
     2161 cgacaatcca gacaggtatc ctctttaagc cgcccgccga tcctaccccg gaggaggcgg
     2221 tcgaggttat cccgccactt cgggaagctg cggacaaatg gttcggcctc aaggacgtgc
     2281 tgctggaggt tgtggatgac cccgaagcgt acctccgctc cgtccgcgac cttgtgactg
     2341 gtcccgaatc gacggccctc cgtgtgggcc aggccgcgtt cacggacgtg cccttctgcc
     2401 tgtccttcaa g
//
LOCUS       DASZYN010000770         6223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_875727, whole genome shotgun sequence.
ACCESSION   DASZYN010000770 DASZYN010000000
VERSION     DASZYN010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875727"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VHE33_19605"
     CDS             complement(<1..466)
                     /locus_tag="VHE33_19605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99716.1"
                     /translation="MHPRDVNAENFAAYPPQGRSLVVAHLETLRQLPLALLPSLLREV
                     IQYDYLFPAERSTIEKQLSALAAMSQPELSQSVKGFAAISLSTDLAEFDWINHPEQFL
                     ERQSAWLWSSHQLDSFRTAANEYSVRIDAATHPEAPPLRRLGIAVIGQGVTAW"
     gene            complement(469..1614)
                     /locus_tag="VHE33_19610"
     CDS             complement(469..1614)
                     /locus_tag="VHE33_19610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99717.1"
                     /translation="MSRIISSPQTPIGLSRSRREFLREAAGLAIGSTLMGASPFLRGE
                     AIRKKRKVIVITFGGGARDQETFAPEGQDNIPHLLSELIPQSSFFSHVTNRGILGHYV
                     ATASLATGVYETLNNFAAVPPEHPTVFEYFRRDLKRPASDTWVVAPSNGFNRIGESDS
                     RSYGAGATVILPKHLLAAASPGANLDHLLRDNYENPLYTPHLSGGEFELQQLDRILRL
                     SVGDFTAHARTLSSPDELSIYIAIRLMQQESPSLLWITLHDIDVAHSGAYSLYIDGIR
                     RTDRLCAQMWRAVQDNPEYAGNTTLFILPDFGRDADYEAGGNGFQHHRTGDAASRTTW
                     MMALGAGVRPGVIYDDPLQSIDLVPSLGAILGFSPAQSQGKPVRELI"
     gene            complement(1621..2829)
                     /locus_tag="VHE33_19615"
     CDS             complement(1621..2829)
                     /locus_tag="VHE33_19615"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT99718.1"
                     /translation="MHELLGLSYAAMSENAKALEQLEIAVQLKPDSAAARTNLGAMLL
                     EAGEADPTAEQFHKALQLEPGNYDANHNLGELYVRAGQLAKAQPMLARAWRARPNAYD
                     NGYDLAMADFLLGRLNEAGQVIVDLAKQKNTGELHNLLAQIDEKQGKFVDAANEYQTA
                     AHLDPTEGNLFDWGSEMLLHRTYDPAITIFEDATRRYPNSPRLFIGLGLALYSRARYD
                     DAVKALLRAADLDPSDPRCYLFLSKAYGSSPGNAAAVTERFRRYAEEQPNNARAQYYY
                     ATSLWKVKRTGGSAEDLSTVESLLKKSIMLDDSFADAHVQLGDFYADQHQYEQSIPQY
                     ERALALDSSLSDAHYRLGMDYVHVGKKEQAQKELAVYQKLRAEHLAGLDKTEVRQFVY
                     SAKAPNPSTP"
     gene            complement(3190..>6223)
                     /locus_tag="VHE33_19620"
     CDS             complement(3190..>6223)
                     /locus_tag="VHE33_19620"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT99719.1"
                     /translation="KGEYRFNQFGATAGGPILRNKFFFFGDYQGTRRVQGNSANTTVP
                     TVAEQTSNFQNLQDILTQQAGAAKTDILGRSIQRGTILDPATTRFVAAGAVDPVSGRT
                     NTSGSDGYVRDPFSTACAPGTASFTLAACPDLNMLPASRIDPNAVKLLQLFPAPNAGL
                     GSYQTSPALFEHSNEFDTREDFNPDASDQVFARFSYSDDPQFIPGPFKGIADGGAFQQ
                     GLQTAKSAQMVAAYTRVFTPNVVNQVRAGFAHLHTTRFGPVGNQMGIPAQYGIQGIPQ
                     VSENGGLPAIVITNLQTLGSNQYLPSDEATATLQITDDFTRIYGKHSFKMGIEYQNAK
                     FSTLQPATSHGRFEYSGTYTDIPGQGANTGGLAQFLLPPTAAPATIAGNPNPNGYSYS
                     GGSNNVSASNINKTYDAKKYFAAYFQDDWKMTPKLTLNLGVRYDYFGPINETNGGQAN
                     FVPIPLPGEGLGNPTFIVPASGKDNRTLSTNGSCFGAGCYGLVDLLAADGITLDVTNK
                     YGQGLLHTQKANFSPRLGLAYQFTNKLVGRGGFGMFFNSFENQGYGPNIGENYPFVFN
                     FGYGVTADPSDPHASIVSQVAPVSYNTSFAGCPTAGPGGTASVEAGFSCIAFDPALVN
                     AKGLGLQGLQFNYQTPRTYAANVTLEYSILPSMAVTASYVYTHDADLQHGVGYHNVSQ
                     ILPANVTTGTGCPYGNFAVGTAPGKLSCYPFPDFGGGSYQATDGISSYHGLQTKLQQQ
                     ISHGLTFLFTYTWSKAMSDAGDLLNGTSADTLRAYAVPGLGPKFDYSLSDFDIKNVIH
                     FSGIYQLPFGKGREFMNQGGIANAVLGGWSTNWILTLQGGQPLGFGCHTSTASGLGCN
                     DIVTGDPKIGAHVMTSGGIHGPFWFGNPAAFTQACLLGGTPGALSPIPGSAPNCVPLT
                     GAAALGYKRDQTWGPGIHNLDFSVFKTFPFRERYSVEFRSEFFNIVNHPNFNQPNFGG
                     NGVVAIGGSGDYTNATFGAIGSTRTPPRQIQFALKLYY"
BASE COUNT         1241 a         1739 c         1984 g         1259 t
ORIGIN      
        1 tccacgcggt tacgccctgt ccaatgacgg caattcccag acggcggagc ggcggcgctt
       61 cggggtgagt agcggcatcg atccggacgc tgtactcgtt cgccgcagtc cgaaaagaat
      121 cgagttgatg actgctccag agccaggcgg actgccgctc cagaaactgt tccggatgat
      181 tgatccagtc gaactcggcg agatcggtcg agagggatat ggccgcgaaa cccttcacag
      241 actgggagag ctccggctgg ctcatggcgg caagagcgga taattgcttc tcgatggtcg
      301 agcgttccgc cggaaacaag taatcgtact ggatgacttc gcgcagcagg ctgggcagga
      361 gagcgagcgg aagctgccgc agtgtttcca gatgagcgac gacgagggaa cggccctgcg
      421 gcgggtaagc agcgaaattt tcggcgttca cgtcgcgggg atgcatgatc aaatcagctc
      481 ccggaccggt ttgccctggc tctgcgctgg cgagaagccg aggatggcgc cgaggctggg
      541 gaccaaatcg atcgactgca gcgggtcgtc gtagatcacg ccgggacgga caccggcacc
      601 caacgccatc atccaggtcg tgcggctggc tgcatcgccg gtgcggtggt gctggaagcc
      661 attgccgccg gcttcgtaat cggcgtcgcg tccgaaatcc ggcaggatga acagggtagt
      721 gttgccggcg tattcgggat tgtcctgcac ggcgcgccac atctgggcgc aaagacggtc
      781 ggtgcggcgg atgccatcta tatataagga gtaagccccg gaatgggcga cgtcgatgtc
      841 gtgcagggtg atccagagca ggctcggcga ttcctgctgc atgaggcgga tggcgatgta
      901 gatcgacagc tcatccggac tggagagcgt acgagcatgc gcagtaaaat ctccaacgct
      961 gaggcgcagg atgcggtcta gctgctggag ctcaaattct ccaccgctga gatgcggggt
     1021 gtacagcggg ttctcgtagt tgtcgcgcag caggtgatcg aggttcgcgc cggggctcgc
     1081 ggctgccagc aggtgcttgg gaaggatgac cgtggcgccc gctccgtacg atcggctatc
     1141 gctctcgccg atgcggttga agccgttgct gggggcgacg acccaggtgt cgctggcggg
     1201 ccgcttaaga tcgcggcgga aatattcgaa taccgtggga tgttccggcg gaacggcagc
     1261 gaagttattc agcgtctcgt agacgccggt ggcaagactg gcggtggcga catagtgacc
     1321 gaggatgccg cgattcgtta cgtgcgagaa gaagctggac tgcggaatca gttcgctcag
     1381 gagatgcgga atgttgtcct gaccttcggg cgcgaaggtt tcctgatcgc gcgctccgcc
     1441 gccgaatgtg atcacgatga ctttgcgctt tttgcggatg gcttccccgc gcagaaaagg
     1501 gctggctccc atcagagtcg aaccgatcgc gagaccagcg gcttcgcgca gaaattcgcg
     1561 gcggctgcgg ctgaggccga tgggagtctg cggcgatgaa atgatgcggc tcatgttcag
     1621 tcagggtgtt gacgggttgg gcgctttcgc ggagtagaca aactggcgaa cctcggtttt
     1681 atcgagcccg gcaagatgtt cggcacgcaa tttctgataa acagcgagtt ccttctgcgc
     1741 ctgctctttc ttcccgacgt gcacgtaatc catgccgagc cgataatgcg cgtcggagag
     1801 actggaatca agagcgagcg cccgctcgta ctgcggaatg gattgctcgt actgatgctg
     1861 atcggcatag aagtcgccga gctgaacatg cgcgtcggca aaggaatcat cgagcataat
     1921 ggatttcttc agcagcgatt cgaccgtgct gagatcttcc gcggaaccgc cggttcgttt
     1981 gaccttccag aggctcgtcg cgtagtaata ctgcgcgcgc gcattgttcg gctgctcctc
     2041 agcatagcgg cgaaagcgtt cggtgacggc ggcagcattg cccggcgaac tgccgtaggc
     2101 tttggagaga aatagatagc agcgcgggtc ggagggatcg agatccgccg cgcgcaacag
     2161 agcctttacg gcgtcgtcgt aacgcgcgcg ggaataaaga gcaagcccaa ggccgatgaa
     2221 cagacgcggt gagttcgggt agcgccgcgt cgcgtcttcg aagatggtga tcgccggatc
     2281 gtacgtgcga tgcaggagca tttcactgcc ccagtcgaag agattgccct ccgtcgggtc
     2341 caggtgggcg gccgtttggt actcgttggc cgcatcgaca aacttaccct gcttctcgtc
     2401 gatttgtgca agcaggttgt gcagctcacc agtatttttc tgttttgcca gatccacaat
     2461 gacctgcccc gcctcattca accgccccag caggaagtcg gccatggcca ggtcgtagcc
     2521 gttgtcgtag gcgttcggtc tcgcccgcca ggctcttgcc agcatcggct gtgctttcgc
     2581 cagctggccg gctcttacat acagctcgcc gaggttgtgg ttcgcgtcgt agttgccggg
     2641 ctccaactgc agcgccttat ggaactgctc ggcggtcggg tctgcctcgc ccgcttccag
     2701 caacatcgct cccaggttag ttcgcgcggc cgccgagtcc ggctttaact ggacagcgat
     2761 ctcaagctgc tccagagcct ttgcgttttc ggacatcgcc gcatagctca gcccaagcag
     2821 ttcgtgcact tgaaagcttc tgggcgcgat gggaaggaca ccttccagcc gatgtgcggc
     2881 ttcggcgaac cggcccgcgt cataatcagc ctgcgcagtt ctacaggcag cctcaaggcc
     2941 tggcttcgac gtagcctgag cgcagagaag cggaacgcag gccgataacg caagccatac
     3001 caaccccctg agccgaggct tcctgctcaa aacctgcctc ccggttctat ccccctgagc
     3061 aaaggtactc tcaagaacaa gaccccggca gggcacccgt gatgcgtgcc atgccggggt
     3121 gatgtccatc ttatatcggc ctctcgaccg ccgtgaacca gcaactgctc tcaggctccg
     3181 ttcacgggct tagtaataca gcttcaaagc aaactggatc tgccgcggtg gcgtgcgggt
     3241 cgatccgatg gctccgaagg tagcgttggt gtagtcgccc gaaccgccaa tcgccaccac
     3301 accgtttccg ccgaagttcg gctgattgaa attcggatgg ttgacgatgt tgaaaaactc
     3361 cgagcggaac tccacggagt agcgctcgcg gaacgggaag gtcttgaaca ccgagaagtc
     3421 gagattatga attcccggac cccatgtctg atcgcgcttg taaccaaggg ctgcagcacc
     3481 ggtcaggggg acgcagttgg gcgccgaacc cggaatcggg cttagtgctc ctggcgttcc
     3541 gcccagcagg cacgcttgtg taaaggcggc cggatttccg aaccagaaag gtccatgaat
     3601 cccgcccgag gtcatgacgt gggctccgat cttcggatcc ccggtcacga tgtcattgca
     3661 cccgagacct gaagcagtgc tggtatggca cccaaaccca agcggctgcc cgccctgcag
     3721 agtcaggatc cagttggtgg accagcctcc aagaaccgcg ttggcgatgc ccccctggtt
     3781 catgaactct ctacccttgc cgaaaggcaa ttggtagata ccgctgaagt gaatgacgtt
     3841 tttgatgtcg aaatccgaca gcgagtagtc gaacttcggg cccaaaccag gaacagcata
     3901 ggcacgaagc gtatcggcgc tggtgccgtt cagtagatcg ccggcatcgc tcatggcctt
     3961 cgaccacgtg taggtgaaca ggaatgtcag accgtgcgag atctgctgct ggagcttggt
     4021 ctgcaatcca tgatacgaac tgatgccgtc ggtagcctga tagctgccgc cgccaaaatc
     4081 cgggaacgga tagcagctca actttccggg ggcagtgccc accgcgaagt ttccgtaggg
     4141 acatcccgtg ccggtagtca catttgcggg cagaatctgc gacacgttgt ggtagcccac
     4201 tccatgctgc agatccgcgt cgtgtgtgta cacatacgac gccgtcacag ccatcgaggg
     4261 caggatcgaa tactcgaggg tcacgttagc ggcgtaggtg cgcggagtct ggtagttgaa
     4321 ctgcagaccc tgcaggccca gtcccttggc attgaccaga gccggatcaa aggcaatgca
     4381 cgagaagccg gcctcgacgg atgcggtgcc gcctggacca gcggtggggc agccggcaaa
     4441 ggaagtgttg tagctgaccg gcgcaacctg cgaaacgatg gacgcatgcg ggtcggaagg
     4501 atcagccgtt acgccgtagc cgaagttgaa aacgaaggga tagttctcgc caatgttggg
     4561 tccgtatccc tggttctcaa acgagttgaa gaacatgcca aaaccgccac gcccgacaag
     4621 cttattggtg aactggtaag ccaggccaag acggggggag aaattggctt tttgcgtgtg
     4681 caggagcccc tgtccgtact tgttcgttac atccagggtg atgccatccg ccgccagcag
     4741 gtcaaccaga ccgtagcacc cggctccgaa acagctgccg ttggtggaca gggttcgatt
     4801 gtctttgccg ctggcgggaa caatgaaggt cggatttcca agaccctcac cagggagtgg
     4861 gataggaacg aagttggcct gtcccccgtt ggtctcattg atcgggccga agtagtcgta
     4921 gcggacaccg agattcagcg tcagcttcgg tgtcattttc cagtcgtcct ggaaatacgc
     4981 tgcaaaatac ttctttgcgt cgtaagtctt gttgatgttg gaggctgaca cgttattcga
     5041 gccgccggaa tagctataac cattcggatt cggatttccg gcaatggtcg cgggcgctgc
     5101 ggtgggaggt aacaggaatt gggcgaggcc acccgtattc gctccctgac caggaatatc
     5161 ggtgtaggtg ccactgtatt cgaagcggcc gtgcgaagtg gcgggttgca gggtcgagaa
     5221 cttcgcgttc tggtactcaa tacccatctt gaagctgtgc ttgccgtaaa tccgcgtgaa
     5281 atcgtccgtg atctggaggg tcgcggtcgc ttcgtcggag ggcaggtatt gattgcttcc
     5341 cagagtttgc aggttggtga tcacaatggc cggcaagccg ccattttcgg agacttgggg
     5401 aatgccctgg attccgtatt gagcgggaat ccccatctgg ttgcctacgg gaccgaaccg
     5461 tgtcgtgtgc aaatgagcaa aaccggcacg cacctggttc acaacgttgg gggtgaagac
     5521 gcgcgtgtat gccgcgacca tctgggcgga tttcgcggtc tggagccctt gctggaacgc
     5581 cccgccatca gcaatcccct taaacggtcc tggaatgaac tgggggtcgt cggagtagct
     5641 gaagcgggca aaaacctggt cgctcgcatc gggattgaag tcttcgcgcg tgtcgaattc
     5701 attggagtgt tcgaacaggg cagggctggt ctgataactg ccgaggcccg cattcggcgc
     5761 tgggaagagc tgcaggagtt tcaccgcatt cggatcaatg cgcgaagccg gaagcatgtt
     5821 gaggtccgga caggccgcga gtgtgaagct tgcggttccc ggtgcgcagg ccgtgctgaa
     5881 cggatcgcgc acgtagccgt ccgatccgga agtattcgtc cgccctgaca cggggtcgac
     5941 tgcgcccgcc gcgacaaagc gggtagtcgc cggatcaaga atggtgccac gttgaatgct
     6001 gcggcccagg atgtccgtct tcgccgcccc cgcctgctgg gtgaggatgt cctggaggtt
     6061 ctggaaattg ctggtctgtt cggccacggt cggcacagtg gtattcgccg agttgccctg
     6121 cacgcggcgt gtcccctgat agtcgccgaa gaagaagaat ttgtttctga ggatggggcc
     6181 gcccgccgtt gccccaaact ggttaaaacg atattcgccc ttc
//
LOCUS       DASZYN010000771         6862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_376627, whole genome shotgun sequence.
ACCESSION   DASZYN010000771 DASZYN010000000
VERSION     DASZYN010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376627"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..333)
                     /locus_tag="VHE33_19625"
     CDS             complement(79..333)
                     /locus_tag="VHE33_19625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99720.1"
                     /translation="MWKVVSTWRHQPVFSIRVLSILTFAAVLGTFIARGLRSTTLADF
                     LAGAAVSFSGILMMQVFSLRLSDDKDEPDESRLTGLHLTH"
     gene            450..635
                     /locus_tag="VHE33_19630"
     CDS             450..635
                     /locus_tag="VHE33_19630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99721.1"
                     /translation="MPLSGEAIRVMNLVDDVATTMRRMLALIPALAPDERKRVSEYLK
                     ASEPSAEQVQAALAPQK"
     gene            1491..1721
                     /locus_tag="VHE33_19635"
     CDS             1491..1721
                     /locus_tag="VHE33_19635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99722.1"
                     /translation="MRSELIFGALTHVNNRYQLCQLASKATRKLHKPNTRLQDTMNDV
                     LVRFRETSPAAAAPVTRHEPVTIAQHSQRRAA"
     gene            1858..3108
                     /gene="rho"
                     /locus_tag="VHE33_19640"
     CDS             1858..3108
                     /gene="rho"
                     /locus_tag="VHE33_19640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HVT99723.1"
                     /translation="MTIAELKEKNITELSRIARTLEIPGSSGLRKQDLIFKILQAQSE
                     KEGHIFAEGVLEILPDGYGFLRSPDYNYLPGPDDIYVSPSQIRKFDLKTGDTISGNVR
                     PPHEGEKYFALVKIEAINFESPEETRNKILFDNLTPLYPQERVKMETVRENISGRVMD
                     LLTPIGKGQRGLIVAPPRTGKTMLLQAIANSVTTNHPEVVLIVLLIDERPEEVTDMQR
                     SVKGEVISSTFDEPAARHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYNTIVP
                     PSGKVLSGGVDSNALQRPKRFFGAARNIEEGGSLTIIATALVDTGSRMDEVIFEEFKG
                     TGNMEIILDRKLVDKRVFPAIDIQRSGTRKEELLIPKEELQRIWILRKVMNPLSPVEA
                     MELLVSQLDKRRNNAEFLANMNQL"
     gene            3260..3694
                     /locus_tag="VHE33_19645"
     CDS             3260..3694
                     /locus_tag="VHE33_19645"
                     /inference="COORDINATES: protein motif:HMM:NF021522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HVT99724.1"
                     /translation="MKDRSHAPMAQKSFLPGNDSFAATAAPDGRYGAFFDDDGESAYF
                     YALDLDSDDLILDAVRVYDKVRYPHRKRHASLSIEWSPDGRRCALLLDGAPQAAFDFE
                     AQRGYSHNGLPAPIRRGRNSWPPSDHAWSDAVVDWLDLRKSA"
     gene            complement(3698..5158)
                     /locus_tag="VHE33_19650"
     CDS             complement(3698..5158)
                     /locus_tag="VHE33_19650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT99725.1"
                     /translation="MESSTATDLPVSATALAGEPRQTPLPDILDALKRVPALQGLTDS
                     ELTWLATHGDEVCVSAGTMVFREGDPADKMWILLKGEAHVRRSQGGLALFVGRAGQIT
                     GVLPFSRMKTHGGQGFAATDMWALQIDRDAFPEMLEAIPSMAQRCVSILLDRVREMTR
                     LEQQTEKLNALGKLAGNLAHELNNPASAAQRAASGLIEELKTYGHQKFMLGSLCLDEA
                     HLQGIRSWRCRMEEQAKHRGVNAVTQAQREDELQRWLREHAVEKTWQVAPDLAELGIN
                     TQNLDELSALLGGQHLDVVLSQFASTARTERMAQAMLDSTSRIFDLIRAVKDYSWMDQ
                     APIQEIDLPQSLETTLTMLQSRLEKVEIERRYAPDLPRISAYASELNQVWMSLLENAL
                     DAMHDAGKIVLSVQVSGDFVVVEIWDNGPGIPAEIQARIFEPFFTTKAPGDGLGLGLD
                     TAQRIVRQHRGYIHVESQPGETCFQIRLPIEALQAY"
     gene            complement(5266..5748)
                     /locus_tag="VHE33_19655"
     CDS             complement(5266..5748)
                     /locus_tag="VHE33_19655"
                     /inference="COORDINATES: protein motif:HMM:NF025151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4174 domain-containing protein"
                     /protein_id="HVT99726.1"
                     /translation="MKRALILMVVMATLSGSGYAQAKLTCAVVPKTLDAMKDCYRPLL
                     VFSANGDDARLKRQVARLDEAADDMMDRFVLFAPIVPDGHKVSTPADSPYTILSPEAM
                     KGVRTKFHIPAAEFVVILLDEDGSVMLRSRIPVDADRLNAVIDKTPRRQAEAHRPHAN
                     "
     gene            complement(5797..>6862)
                     /gene="ilvC"
                     /locus_tag="VHE33_19660"
     CDS             complement(5797..>6862)
                     /gene="ilvC"
                     /locus_tag="VHE33_19660"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HVT99727.1"
                     /translation="EDRMAAKTYHDGDADLSLIQQKKVAVIGYGSQGHAHALNLKDSG
                     VDVRVGLPATSKSVDKAKKAGLQVMPVSDAAAWADVIMILTPDHSQGETYAKEIAQHM
                     KKGKTIMFGHGFNIRFGQIKPPADVDVSMVAPKGPGHRVREVFTEGGGVPCLIAVEQN
                     ASGKAKELALSYAKGIGGTRGGVLETTFAEETETDLFGEQAVLCGGTSALVKAGFDTL
                     VAAGYQPEIAYFECLHELKLIVDLMYRGGLAYMRYSISDTAEYGDYTAGPRIVTDQTR
                     AEMKKLLSEIQDGTFAKKWIEENKTGRKNFTEMRKKEAGLLVEEVGATLRAAMPFLDP
                     VVVKDGAPQPAAAAAETVNA"
BASE COUNT         1352 a         2281 c         1904 g         1325 t
ORIGIN      
        1 atcgccagga acttcggctc gcatgttgcc acgatgctgc ggagttcgcg cgcggcttca
       61 acgtgcgtca tcgcgccttc agtgagtgag gtggagcccg gtcagccggc tctcatccgg
      121 ttcgtctttg tcgtcgctca gacgcaggct gaagacctgc atcatgagga tgcccgaaaa
      181 gctcaccgcc gcaccggcca ggaaatccgc cagggtcgtg gagcgcaggc cccgcgctat
      241 aaacgttccc aaaaccgcag cgaacgtcaa aatcgagagg acacggatcg aaaagaccgg
      301 ctgatggcgc caggtgctta caactttcca catcgaggag aggcgttcca taggccgcag
      361 tttgtcacga atggcgcagt ccggcaatcc cgcctttacc tctacccgtc ccatcgccga
      421 caatgggtcg agaggtcaac caaggtccta tgcccctttc cggcgaagcc attcgcgtaa
      481 tgaatctggt ggacgacgtg gcgaccacga tgcgccgcat gctggctctg attcctgctc
      541 ttgcccccga cgagcgcaag cgcgtcagcg agtacctgaa ggcgtcggag ccgagcgccg
      601 agcaggtgca ggcggcgctc gcgccccaga agtagaagtt cggggcaggc ccgtcgatca
      661 caggccgcgc ggcccattcc gcgcgggagc atccctatct gccgccaggt cgcgttttcg
      721 accgcgcggt ggacctcggg gctgttgcta gtctcgcttt gagagactcc cgccagcgcc
      781 actcacgtga cgtctggatg aacagcggga ggcttccgct ctcctccctt ggtcgaaaca
      841 gcgtcgcgcg cggttacaac aggagtaact cccgcgagag aggtcagctt ccgccaaaag
      901 cgggaatatt cagtagcgga cgccaactat tgccaaatca acagaatagt gaccaaatgg
      961 tgagtaattc gggcgttctc ccgcccccaa tgtacagatt ttggaagtga cggaagagga
     1021 caggtgcatc ctttggttac cctgccgcac cgccaatagc agcgtgatca acggtttacg
     1081 acgacgtttc cattttcggt aactttggta aagcaggtat gaaaaaaatg gttgaccccg
     1141 tttcagccgg agggtatttt caaaactgtc aattgcgact tccgggtccg ccgtcctgct
     1201 aaaaccccgg tcgagcagat ggaagtgact ctccagggag cgcaagccat ccctctgcac
     1261 gattcggcca gtggcccgcg aagtcaaaag agacaatccg cacttcgcag cacgtttttt
     1321 tcagagagag gaaaaaggtg atcaaaatcc ctgctttgtg cgtctaatct ggcagagctt
     1381 ccgggatccc cttcttccca aaacctgctt cgggcccgac gtggttcagc ggtttttctg
     1441 tggtcgtaag cggtcggaca acaaactagt taaaagcaag gagacacatc gtgcgttcag
     1501 aactcatttt tggtgcactc actcatgtca acaatcgcta tcagctgtgc cagcttgctt
     1561 ccaaggcgac gcgcaagctg cataagccga acactcgcct gcaggacacg atgaacgacg
     1621 tgctcgtccg cttccgggag accagcccgg cggcggccgc gcccgtcact cgccacgaac
     1681 ccgtcaccat cgcacagcat tcgcagcgtc gcgccgccta gtgccggcgt gcgctgcctg
     1741 ggtgcggcgt gctctcttcc gctgcgccca catcggttaa cataactcct gagccccgct
     1801 cctccaattc ccttcccaat tcaaaatccc tcctcgaaag ctcaccgcag gtgactgatg
     1861 accatcgcag aactcaaaga gaagaacatt actgaactca gccgcatcgc gcggacgctt
     1921 gaaatccccg gctccagcgg cctgcgcaag caggacctga tcttcaaaat cctccaggcc
     1981 cagagcgaaa aagaaggaca catcttcgcc gaaggcgtgc tcgagatcct gcccgacggc
     2041 tacggttttc ttcgctcccc cgattacaac taccttcccg gccccgacga catttacgtt
     2101 tcgccctcgc agatccgcaa attcgatctc aagaccggcg acaccatcag cggcaacgtc
     2161 cgcccgccgc acgaaggcga aaaatacttt gcgctggtca agatcgaagc catcaacttc
     2221 gaatcgccgg aagaaacgcg caacaagatc ctgttcgaca acctgacccc gctctatccc
     2281 caggagcgcg tgaagatgga gacggtccgc gaaaacatca gcggtcgcgt gatggatctg
     2341 ctcacgccca tcggcaaagg ccagcgcggc ctgatcgtcg cgcccccgcg caccggcaag
     2401 accatgctgc tgcaggccat cgccaattcc gtcacgacca accatccgga agtcgtgctc
     2461 atcgtgctgc tcatcgatga gcgccccgaa gaagtgaccg acatgcagcg ctcggtgaag
     2521 ggcgaggtta tcagctcgac gtttgacgag ccggccgccc gtcacgtgca ggtcgcggaa
     2581 atggtgatcg agaaggccaa gcgtctggtg gagcacaagc gcgacgttgt gattcttctc
     2641 gactcgatca cacgtctggc gcgcgcctac aacaccatcg tgccgccctc cggcaaggtc
     2701 ctctccggcg gcgtcgactc caacgctctg cagcgcccga agcggttctt cggcgccgcc
     2761 cgcaacatcg aagagggcgg ttcgctcacc atcatcgcca ccgcgctggt cgataccggc
     2821 tcccgcatgg acgaagtcat cttcgaagag ttcaagggca ccggcaacat ggaaatcatt
     2881 ctcgaccgca aactggtcga caagcgcgtc ttcccggcca tcgacatcca gcgctcgggc
     2941 acccgcaagg aagagctgct catccccaag gaagaactcc agcgcatctg gattctgcgc
     3001 aaggtgatga acccgctttc gccggttgaa gccatggagc tgctcgtcag ccagctggac
     3061 aagaggcgga acaatgcgga attccttgcg aacatgaacc agttgtaatg cccggctccg
     3121 gcacggggac gaatgaaacg tttgtccccg gtcgagcacc atccaaaggc gcgagtttta
     3181 gacaggacca cgactttggt cctgcgccat tgtctgacaa aaaggtgaca aaccccacag
     3241 gccctcgcgt taatctgaga tgaaagaccg gagccacgcc cccatggcgc aaaagagctt
     3301 tcttcccgga aacgattcct ttgcggcgac agccgcgccc gatggtcgtt acggggcttt
     3361 cttcgacgac gatggcgagt ccgcgtattt ctacgcgctc gacctggact ccgacgatct
     3421 gatcctggac gcggtgcgcg tctacgacaa agtccgctat ccgcaccgca agcggcatgc
     3481 gtcgctctcg attgaatggt cgccggacgg acgccgatgt gctctgctgc tcgatggagc
     3541 gccccaggct gccttcgatt tcgaagccca gcgcggctac tcgcataacg gcctgcccgc
     3601 tccgattcga cggggccgca acagctggcc cccctcggat cacgcctggt ccgatgccgt
     3661 ggtcgactgg ctggacctgc gcaaatctgc gtagcggcta atacgcctgc agcgcctcaa
     3721 tgggcagccg gatctggaag cacgtctcac ccggctgcga ttccacatga atgtacccgc
     3781 gatgctgccg cacgatgcgc tgcgcggtat cgaggccaag ccccagacca tcccccggag
     3841 ccttcgtggt gaagaacggc tcgaagatgc gcgcctggat ttcggccgga atgcccggcc
     3901 cgttgtccca gatctcaacc acaacaaagt ctcccgagac ctgaacgctg agcacaattt
     3961 tgccggcgtc gtgcatcgcg tccagcgcat tttccagcag cgacatccac acctggttca
     4021 gctcgctggc atacgccgag atgcgcggca gatccggcgc atagcgccgc tcgatttcga
     4081 ctttttccag acgcgactgc agcatcgtca gggtggtctc gagcgactga ggaagatcga
     4141 tttcctgaat cggcgcctgg tccatccacg agtagtcctt gaccgcccgg atcaggtcga
     4201 agatgcggct cgtcgagtcg agcatcgcct gggccatgcg ctcggtccgc gccgtcgacg
     4261 caaactgcga cagcaccacg tccagatgct ggcctccgag caacgccgac agttcgtcga
     4321 gattctgcgt gttgatcccc agctcggcca ggtcgggcgc aacctgccag gttttctcca
     4381 ccgcatgctc gcgcaaccag cgctgcagtt catcctcgcg ctgagcctga gtcacagcat
     4441 tgactccccg atgcttcgcc tgctcttcca ttcgacagcg ccacgaccgg atcccttgca
     4501 aatgggcttc gtcgagacac aggctgccga gcatgaattt ctgatgtccg taagttttta
     4561 attcttcaat cagccccgac gcggcacgct gcgccgcgga tgcgggatta ttgagttcgt
     4621 gcgccaggtt tccggccagc ttgcccagtg cgttcagctt ttccgtctgc tgttccagcc
     4681 gcgtcatctc tcgcacccgg tccagcaaaa tggatacgca tcgttgcgcc atcgagggga
     4741 tcgcctccag catctctgga aaagcgtctc gatcgatctg cagcgcccac atgtcggttg
     4801 cggcaaaccc ctggccgccg tgcgtcttca tccgcgagaa gggaagcacg cccgtaattt
     4861 gccccgcgcg ccccacgaac agcgccagcc ccccctgcga gcgccgcacg tgtgcctcgc
     4921 ccttgagaag gatccacatt ttgtccgcgg gatcgccctc gcgaaaaacc atggttccgg
     4981 ccgagacgca cacttcgtcc ccatgcgtgg ccagccaggt cagttcggag tcagtcagcc
     5041 cctgcagcgc gggcacgcgc ttcagcgcgt caagaatatc cggcagaggc gtctgccgcg
     5101 gctctccagc gagagcagtg gcagataccg gaagatcggt cgcagtcgat gactccatat
     5161 gccctcctgg ggggagccca aacgctgggg cgcgtggggt gaggggagag ttggtcccga
     5221 tagaacgatg ctaaatgact gactcctgac gcgcgcgttt tgtgactaat tcgcgtgcgg
     5281 ccggtgggcc tcggcctgcc gccgcggcgt cttatcgatg accgcgttca gccggtccgc
     5341 atcgaccggg atcctcgacc gcagcatcac gctcccatcc tcatcgagca atatcactac
     5401 aaattcggcc gccggaatgt gaaatttcgt gcgcacaccc ttcatcgcct ccgggctcag
     5461 gatcgtgtac ggcgaatccg cgggcgtgct caccttgtgc ccgtccggaa cgatgggggc
     5521 gaacagcaca aagcgatcca tcatgtcgtc ggccgcctca tccagccgcg ccacctgccg
     5581 cttcagccgc gcgtcgtctc cgtttgcgct gaaaaccagc aaaggccggt agcaatcttt
     5641 catcgcgtcc agcgtcttcg gcacaacagc gcaggtcagc ttcgcctgag cgtaaccact
     5701 tccgctcaga gttgccatga ccaccatcag gatcaaagcc cgtttcatgc tgacctgcac
     5761 tgactgtgcc ctgtgacctg cgctttgtgc tctgtcttac gcgttgaccg tttccgccgc
     5821 tgctgccgcc ggctgcggag cgccatcttt caccaccacc ggatcgagga agggcatggc
     5881 cgcacgcagt gtcgcgccca cttcttcgac gagaagcccg gcttccttct tccgcatttc
     5941 ggtgaagttc ttgcgccccg tcttgttctc ttcgatccat ttcttcgcga aggttccatc
     6001 ctggatctcg gagagcagct tcttcatctc ggcccgtgtc tggtcggtca caatgcgcgg
     6061 acccgccgtg tagtcgccgt actccgccgt atcggagatg gagtagcgca tgtacgcgag
     6121 gccgccgcga tacatcaggt cgacgatcag cttcagctca tgcaggcact cgaagtaagc
     6181 gatctccggc tgatagccgg cggccaccag cgtgtcgaac ccggccttga ccagcgccga
     6241 cgttccgcca caaagcacgg cctgctcgcc gaacagatcc gtctcggtct cctcggcgaa
     6301 ggtcgtctcc agcacgccgc cgcgcgttcc accgatgccc tttgcatacg acagcgccag
     6361 ctccttcgcc ttgcccgagg cattctgctc cacagcaata aggcacggca cgccgccgcc
     6421 ttcggtgaac acttcgcgca cgcgatgccc agggcccttc ggcgccacca tcgacacgtc
     6481 cacatcggca ggaggcttga tctgcccgaa gcgaatgttg aacccgtgcc cgaacatgat
     6541 cgtcttgccc ttcttcatgt gctgggcgat ctctttggcg taggtctcgc cctgcgagtg
     6601 atctggcgtg aggatcataa tcacgtcggc ccaggccgct gcatcgctca ccggcatcac
     6661 ctgcagcccg gccttcttcg ccttgtccac ggacttgctc gtcgcgggga gacccacgcg
     6721 tacatccacg ccggaatcct tcaggttcag cgcatgcgca tgtccctgcg agccgtaacc
     6781 aatcacggca accttcttct gctggatcag cgacaaatct gcgtcgccat cgtgatacgt
     6841 cttcgctgcc attctgtcct cg
//
LOCUS       DASZYN010000772         1523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_426895, whole genome shotgun sequence.
ACCESSION   DASZYN010000772 DASZYN010000000
VERSION     DASZYN010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426895"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(773..1351)
                     /locus_tag="VHE33_19665"
                     /pseudo
     CDS             complement(773..1351)
                     /locus_tag="VHE33_19665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          307 a          507 c          432 g          277 t
ORIGIN      
        1 aaggcgccag ctatcggctc cgcggcagcg gcatcgacag cctccccagc atcagaacgc
       61 aagatcaggc agactgacca cagcaaccgg tggccctatt ttcagccgtc gcttcggtcc
      121 taccttcgag cgttgccgac agcatgccat gggcctctcg cgagcacatg gttgcccgtc
      181 gaagtccatg cttcgttggt gttcctacga atccgtccag cggcgtccgc tggctgccat
      241 cagaactcgc ccgggatgtg cctcaacatg tgcctcaggc gaccgccaaa caggtgagga
      301 cacgatccgg ccagtaccca cttgattcac acgccgaatt acgacttgga gcgctcctcg
      361 ttctgcttcc accgccagtc caacgcccat acgcccatca aagcggcaaa gagcgctacc
      421 accacgatca caccaagcgg atctggcagc cgccaagctg cagcggccat tgcgatcaag
      481 atcacggcgg caatggcccg accccgacgt gttacgactg ccgagcgcgg acgcatgagc
      541 cgctggccaa gtggccgatc gtcgacactg ggcccaaata cacggtcatc cagctccgac
      601 gcgcgggtca tccataccgc gaaggacggt ccttgcctct tttgcgccga accctccgtc
      661 gcttgattcg catccactcg gccccgggtg ccgcgccgac ccttgacatc cataacgatt
      721 cccttcctga aaatcggacg cggcatagtg agcgcagtct gttcttcgcc agctagttcg
      781 ccggctgggg cgggttgtcc agcgtatcga tgaagtgaag gaagatccga gaaccagctc
      841 gggacataac ggaggacaag cccgcagtca cgagcccgaa tttgagctca tcaagaagcc
      901 gagcgcggtc cgcatccgtg tagtgacgga tctgtagtcg ctcacccgcg acctgcagaa
      961 ccgtgccgag ggcgccgccg atgccagctt gagggcccgg aatggcggtc gagacccagc
     1021 cgatcccgaa cccggcgtcc ttcgcgatct cgccaaccgc atggttaatc cgatcaccga
     1081 tactgaggcc accgttacag gtacacctgc catgcaggtt cagatgccga aacgctctca
     1141 agagcttcct gaacgtgtgt ttcacaccac cgccgccgtc aagcatgatc tgtgttccgc
     1201 caaggtcgga ggcgttgatc ggatcctggt tgcagtagtc gtacgcgttt gcgctaccac
     1261 caccgacgct gtcaaccgaa gtgaaccgcc caagctgcgg aacgtacccc cggatcccca
     1321 tcagcacgag accgccgatg ctgccggcat cacgctggtg tccgccaagc cagccatagc
     1381 gtgtgtgcgg ccccccgttt ttcggtccat ttcgcttgag agtctgactt caagtgagag
     1441 ggctggtgtg agtttctgat ggctgcagcg aaacggttgt caccggagca gatcgtcgcg
     1501 aagttgcggg aggcggagcg act
//
LOCUS       DASZYN010000773         4272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_875923, whole genome shotgun sequence.
ACCESSION   DASZYN010000773 DASZYN010000000
VERSION     DASZYN010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875923"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4272
                     /locus_tag="VHE33_19670"
     CDS             <1..>4272
                     /locus_tag="VHE33_19670"
                     /inference="COORDINATES: protein motif:HMM:NF042646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6603 domain-containing protein"
                     /protein_id="HVT99728.1"
                     /translation="DLATALDLYDAAGKFTGHATQWKTLLDGNWSAAIVGPARTAAST
                     AISEVIQAVSPAALGASGVSVNAGWDSQPTVSLDATGLAIPGGAVLADLELGYKNGGI
                     VAKAGLSLNLNLMPAVKAVPKLTIEFSGGKFQIGIDVGTTDVVGDLLLPLAADLVMTA
                     EKSAFGTPIWTGGPTVLQLLQDAKLATSTGDVATPLPSVVDIVSGLATGLATQASFNV
                     GDLKIAFVSQGSQIGVRLTGSQSIPNDEVDLSVQFGNTDFSNSGLTVWVLNGNQFAPS
                     LDVRGLGIKLGGASGGPLINSSGFRLQDIGGYIFFTFDGALNNLGGALDAHGVGLPLN
                     QLGGSNDGGNAVASSLLEGMKAPSGDPNPVNPAVDVLLSYIDGMFDITLGGKSPIWIG
                     VHRAFGPVYIDQLGVEWDNSSASMLVDASIQVGGLTVQAYELSLTAPFKSLREPENWK
                     LDLMGLALGIDAGPVSIAGGLAKNPGNPIDYDGMLSAVVAGIGFTVVGAYSRPADSQG
                     SYTSLFIFVSLPIPLGGPPFLFVTGLGGGAGFNRQLLQPTDMNQIPDYFLVKAIDDSS
                     LANDPMSALVSMGKFVKPNRGAFWFAAGVRFNSFVVVNCVVVVWVSIDHGFELGALGV
                     GRMQLPYADIALVSIELALALKYSESEGYFGCRAQLTDNSWIFSSDCQLTGGFAFYIF
                     FKTGHFVLSMGGYHPAFNKPPEFPDIPRLGFHWQVLDFVLIKGESYFAITSSAFMCGG
                     RLEASASIGGIRAWFTVHCDILIQWDPFHYDFDAGIEVGVSLTIHVCFFGACADIGIT
                     ISKGADIHIFGPPFHADLTFDAYITTITLSFGGDPHPAPDPLTWNDFRTKYLVSGNPE
                     NTWVAVRMTSGLLLPDPPGASPSPGTSDDPWKLNPEWAFVTESRMPASGYSAGGALDN
                     TGAVIQLPFATKGDSKSWDLAPMDVLKVGSNHTFTITPPVTHPDQFQVSEITTLLPEA
                     TWVYYDPSNLPAAANRINAITGLSVTAVARLQGKSGLIPIGTLVDDDPRYAQPLPFAT
                     VIAVVGTLQTYGLTSESLAAITAGASGTQVLQAATTVLAGDDPFFSTARATAGIPAGG
                     LSPLAVRALRKYRSAPPLLTPLATGLSMKPVELALPPPFYRPPSLDPVALDELRLRAV
                     MLHPAAPVVDAPTASRTTVKKAQANTPRMAPPRLQILAGARLDFVPAANAPRPTATTF
                     GVRSLRNPAVGALSGIAHADAFSKAATQVAGDGVTVPAGTTHIWELPDTGYFITLRGH
                     SAVRVTAMNRAGQTIQDIETVVVQSKQIAVPSGTEHLAITCLGDLQSASAVQDGEGSV
                     TWAAGAGGDNPAVGWQTGNLFAQVGAASILARGSTLRLRKAYLPVVNRQKTSQSMVRI
                     SDALIEQSGAETWLPTAVSVIMILLDRADANAAESGDIGISCDGAT"
BASE COUNT          762 a         1318 c         1309 g          883 t
ORIGIN      
        1 gatctcgcga cggcactcga tctctacgac gcggccggta aatttaccgg gcacgcgaca
       61 cagtggaaga ctctgctcga cggcaactgg agcgccgcta ttgtcggacc agcgagaacg
      121 gccgcatcga cggcgatcag cgaagtgatt caggccgtca gccctgccgc actcggtgcg
      181 tcgggagttt cggtcaacgc gggctgggat tctcaaccca ccgtttccct ggatgcgacc
      241 ggactcgcga ttccgggcgg cgcggtattg gccgatttgg agcttggcta caaaaatggc
      301 ggcattgtcg cgaaagcggg cctcagcctc aacctgaacc tcatgccggc ggtgaaagcc
      361 gttccgaagc tcacgattga gttctcggga ggaaagtttc agatcggcat cgacgtggga
      421 acgaccgacg ttgtcggtga tctattgttg ccgctcgccg cagatcttgt gatgacggct
      481 gaaaagagcg cattcgggac gcccatctgg accggcgggc ccacggttct ccaactcctg
      541 caggatgcga agctggcaac atccaccggc gatgtggcca caccgctgcc ctctgtcgtc
      601 gatatcgtga gtggcctcgc gaccgggctg gctacgcagg cttcgttcaa cgtgggcgat
      661 ctgaagatcg cgttcgtcag tcagggcagc cagatcggcg ttcgcctcac cggttcgcaa
      721 tcgatcccca acgatgaagt cgatctttcc gttcagttcg gcaacacgga tttttcgaat
      781 tcgggactca ccgtgtgggt gctgaacggc aaccagttcg cgccttctct cgatgtgcgc
      841 ggactgggga tcaaactggg cggagcctcc ggcggcccgc tgatcaatag ctccggattc
      901 cggcttcagg acatcggggg ctatatcttc tttaccttcg acggtgccct caacaatctt
      961 ggcggagcgc tggatgccca tggcgtgggt ctaccgctga atcagctggg cggaagcaac
     1021 gatggcggga atgcggtcgc atcgagcctg ctggaaggca tgaaggcccc gagcggcgat
     1081 ccgaatccgg tgaacccggc tgtggacgtg ctgctcagct atatcgatgg gatgttcgat
     1141 atcacgctgg ggggaaagag cccgatctgg attggcgtac accgcgcctt cggcccggtg
     1201 tacatcgatc agctcggcgt ggaatgggac aattcgtctg cctcgatgct ggtggatgca
     1261 agcatccagg ttggcgggct cactgtgcag gcgtacgagt tgagcctcac cgcgccgttc
     1321 aaatctctgc gcgaaccgga gaactggaag ctggatctga tgggactggc cctcggcatc
     1381 gatgccgggc ctgtatccat tgcgggcggg ctggcaaaga atcccggcaa tccgatcgat
     1441 tacgacggca tgttgagcgc cgtggttgcc ggcattggat tcaccgtcgt cggcgcctat
     1501 tcacggcccg cggattcgca gggctcttac acttcgctct tcatctttgt ctcgcttccc
     1561 attcctctcg gtggtccccc cttcctgttc gttaccggcc tgggcggggg tgcaggattc
     1621 aatcggcagc tgctgcagcc gaccgacatg aaccagatcc ccgattattt cctggtgaag
     1681 gcgatcgacg acagcagcct cgccaacgat cccatgagcg cgctggtctc gatgggcaag
     1741 ttcgtcaagc cgaaccgcgg cgctttctgg tttgcagccg gcgtgcgctt caattcgttc
     1801 gtcgtggtga actgcgtcgt cgtggtgtgg gtgtcgatcg accacggctt tgagctgggg
     1861 gcgctcggcg tcggccgcat gcaactgccc tatgcggaca tcgcgctggt gagtatcgag
     1921 cttgcgctgg cgctcaaata cagcgagtca gaggggtact ttggctgccg cgcgcaactg
     1981 actgacaact cctggatctt cagcagcgat tgccagctga ccggtggatt tgcgttctac
     2041 atttttttca agaccggcca cttcgttctc tccatgggcg gatatcatcc cgcgttcaac
     2101 aaaccgcctg agttccccga cattccgcgg cttggattcc actggcaggt tctggacttc
     2161 gtcctcatca agggcgagtc gtattttgcg atcaccagct cggctttcat gtgcggaggg
     2221 cggctggagg cgtcggcgag catcggcggc atccgcgcat ggttcacggt gcattgcgac
     2281 atcctcatcc agtgggatcc gttccactac gatttcgacg ccggcatcga agtcggcgtc
     2341 tcgctcacca ttcacgtttg cttctttggc gcgtgcgcgg atatcggcat cacgatttcg
     2401 aagggcgccg acatccacat cttcggaccg ccgttccatg ccgatctcac gtttgacgcc
     2461 tacatcacga cgatcactct gagtttcgga ggcgacccgc atcctgcccc cgatccactc
     2521 acatggaatg atttccgcac caagtacctg gtgtccggga atcctgagaa cacgtgggtt
     2581 gcggttcgca tgacttccgg cctgctgctg ccggatcctc caggagcgtc gccttccccc
     2641 ggaacgagtg acgatccatg gaagctgaat ccggagtggg cgttcgtcac cgagtcgcgg
     2701 atgccggcct cgggctactc tgccggggga gcactcgaca acacgggcgc cgtcatccaa
     2761 cttcctttcg ccacgaaggg agacagcaag tcgtgggatc tggctccgat ggatgttctg
     2821 aaagtcggaa gcaatcacac gttcacgatc acaccgcccg ttacccatcc cgaccagttc
     2881 caggtatcgg agatcacaac actgctgcct gaggcgacct gggtctacta cgatccgtcg
     2941 aacctgcccg ctgctgcgaa ccgcatcaat gccatcacgg gactgagcgt cacggccgtt
     3001 gcaaggctgc aggggaagag cgggctgatc cccatcggaa ctcttgtcga tgacgatccg
     3061 agatacgcgc aacccttgcc gttcgccacg gtgatcgctg tggtggggac tctgcagact
     3121 tacggcctca cgtcagaaag ccttgccgca atcactgcag gcgcgtccgg gactcaggtg
     3181 ctccaggctg caacgacggt tctcgccggc gacgatccat tcttctccac agcgcgcgcg
     3241 accgcgggga ttccggcggg gggactttcg cccctggcgg tacgcgctct tcggaaatat
     3301 cgctccgctc cgccgctgct gacaccgctg gccaccggcc tttcgatgaa gccggtagag
     3361 ttggctctgc cgccgccctt ctatcggccg ccttcgctcg atcctgttgc gctggacgag
     3421 cttcgtctac gtgcggtgat gctgcacccg gccgctcccg tcgtggatgc gccgaccgct
     3481 tctcgtacga cggtgaaaaa agcgcaagcc aacacgcccc gcatggctcc gccgcgactg
     3541 cagattctcg caggagcgag gctggatttt gtacctgcgg ccaacgctcc ccggcccact
     3601 gccacgacgt ttggcgtgcg cagtctgcgc aaccctgcag tgggggcgct gtcgggtatc
     3661 gcccatgccg acgccttttc gaaagcggcc acgcaggtcg caggagatgg cgtcacggtc
     3721 ccggctggaa cgacccacat ctgggaattg ccggacaccg gatatttcat cactctccgc
     3781 ggacattctg cggtgcgcgt gaccgccatg aatcgcgcgg gacagacgat ccaggacatt
     3841 gagaccgtcg tagttcaatc gaagcagatc gcggtgcctt cgggcaccga acatctcgcc
     3901 attacctgtc tcggcgatct gcaatccgca tctgcggttc aagacggcga aggctcagtg
     3961 acctgggcgg cgggtgcagg cggcgacaat ccggcggtgg gctggcaaac cggcaatctg
     4021 tttgcgcaag tcggtgcggc ttcgatcctg gcacgcggct ctacgctccg cctcaggaaa
     4081 gcgtatctgc cggttgtgaa tcggcagaaa acaagccaga gcatggtcag gatttccgat
     4141 gcgctcattg agcagagcgg cgcggaaaca tggcttccaa cggcggtctc cgtcatcatg
     4201 attctgctgg atcgcgccga tgcgaacgct gcggagagcg gcgatatcgg gatttcctgc
     4261 gacggggcta ct
//
LOCUS       DASZYN010000774         3333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_178688, whole genome shotgun sequence.
ACCESSION   DASZYN010000774 DASZYN010000000
VERSION     DASZYN010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178688"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VHE33_19675"
     CDS             complement(<1..342)
                     /locus_tag="VHE33_19675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99729.1"
                     /translation="MEYSDAPEEVSLARDAHRPRLRFRALVAGTVAAVVLIALIAIGS
                     RGFRWFDAALIGYAVATVFATAAVIYKYTFWLARPPTWRYWRRSWQLFFSASNFRRYA
                     LLIPAAIGDLFT"
     gene            complement(1288..2400)
                     /gene="thiO"
                     /locus_tag="VHE33_19680"
     CDS             complement(1288..2400)
                     /gene="thiO"
                     /locus_tag="VHE33_19680"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HVT99730.1"
                     /translation="MDKQAEVLIIGGGIVGCAIAYFLRKARVEVSVFERDEIGGHASR
                     AAAGLLAPLGPLSGPGPLAELVLAGFALLPDLLKELEELSGLDIGFVRCGALRVVRNP
                     RRVAHLHKRLHNWLPLGLELTWLDGEEARHTEPHLAGDIRAAIHAPEEAQLEAPRLVQ
                     AFALAAQRLGARLYPQREVVELLRSRSRITGIRTRDGEIVPCAQLVLASGAWTSTWSE
                     VLQTTLPISPLHGQLLTMPQLPIPLRHMIFGEGIYLAPRARSIIVGATREEQGFEIGV
                     TAEGLASLKAAAARLVPALAGSPVSTARAGLRPKTADSRPIFAHLPRWENVLLAGGHN
                     SIGLILSGITGREVAHMLITGQTPPLLQAFSLARFP"
     gene            complement(2844..>3333)
                     /locus_tag="VHE33_19685"
     CDS             complement(2844..>3333)
                     /locus_tag="VHE33_19685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99731.1"
                     /translation="TLGSTGSSSSSGGESQTGGSGASALGTVIACLVGLVIIGGLLFL
                     FTRLFRRRGNTFTGNRGYVNPNYDAGYPPNYNQNYGPGYPQRNGMNPWMAGGLGAAAG
                     GFLGYELGKEAGEREERRHEDYSGGSDNFGGGSSGDFGGGFGGDFGSSGDFGGGGSSG
                     DF"
BASE COUNT          710 a          975 c          989 g          659 t
ORIGIN      
        1 cgtgaacaga tcgccaatcg cggcgggaat gagcagcgcg tagcggcgga aattggaggc
       61 agagaaaaac aactgccagc ttctccgcca gtagcgccag gttggcgggc gggccagcca
      121 gaaggtatat ttataaatca cagctgccgt cgcgaagacc gtcgccacag catagccgat
      181 caaagccgcg tcaaaccagc ggaagccccg cgaccctatg gcgatcagcg caatcagcac
      241 cacagcggcg acagtgcccg ccaccagagc gcggaaacgt agccgtggcc ggtgagcatc
      301 tctcgccagc gacacctctt caggagcatc ggagtattcc atagtgccat cctattctac
      361 aaggatacag gccagagtgc ctgcatcttc ctctaaaaga atcgtaacat atagtgtgta
      421 gagatggaag cacagctatc agatccaatc atgcctcttt tctctgctgc tgtgctttgt
      481 aaggaggcca cccgctatga cctgccagaa tgtgtaaaag tgtggaagtt tggaagtttg
      541 ggctgtggag cggcctgtag agcggcgtga gagaaacttc cacgaaactt ccacgacctc
      601 tttgcctgga agtttggaag tttcccgttg cggaactgaa atcgtctcaa atgagaaaga
      661 gggccgcttc agcgggctct gtccggcttc agccgggacg cccgccacgc ctcctctgcc
      721 gaagcaacgc aaccaggcgc gcggggacgc cgatgacgcc gccagcggtg gtctggttgg
      781 ggcgagatgg cggggactcc aggcgtcccg gctgaagccg gggagggcac gctgaagcgg
      841 ccctctttca ggtacttaga gcaatacaat ataaatatta ataatatatt acgtaatagg
      901 ttgtatagta atatgtgctt cattctatga ttgcgtgggt tagagagtaa gtgatagaag
      961 atacatgaga gagagaatgc ttcttcatca aacttccaaa cttccaggca aagaggtcgt
     1021 ggaagtttcg tggaagtttc tctgacgccg ctctacaggc cgctccacag cccaaacttc
     1081 caaacttcca cgatatacct cttttcgtct tcggaccgcg ttcctgggcc caaacttcca
     1141 aacttccact ccatgaggcc atggaagttt cgtggaagtt tctctgacgc cgctctacag
     1201 gccgctccag ggcaaatcct tccaaacttc cacacagtac acattctgcc agttgccgcg
     1261 agcagtaaag cttagggaac tcaaccttta cggaaagcgg gcaagggaaa acgcttgaag
     1321 caaggggggt gtttgtcctg tgatgagcat gtgcgcgacc tcgcggcctg tgatgccgct
     1381 gaggatgagg ccgatgctgt tgtgaccgcc ggcgaggaga acattttccc agcgcgggag
     1441 gtgagcgaag atgggcctgg agtcggcggt tttgggacgc agaccggccc gggctgtgct
     1501 gaccgggctg ccggccagcg cgggcacgag gcgggcagcg gcggccttta agctggccag
     1561 tccttctgct gtgacgccta tctcgaagcc ctgttcttcc ctggtcgcgc ctacaatgat
     1621 gctgcgcgca cgcggggcga gataaatacc ttctccgaag atcatatggc gcaagggaat
     1681 gggcaattga ggcatagtca gcaattgtcc atgtaaaggg ctgatgggca gcgttgtctg
     1741 gagaacttcg ctccaggtag aggtccaggc tccgctggcc agaacgagct gtgcgcaggg
     1801 tacaatctcg ccatcccggg tacgaatgcc cgtgattctt gagcggctgc gcagcaactc
     1861 gacgacctcg cgctgtgggt acaggcgcgc tcccagtctt tgcgcggcca gggcgaaggc
     1921 ctggaccagt cgcggcgcct ccagttgcgc ctcctcgggc gcgtgaatgg cggcgcggat
     1981 atccccggcc aggtgcggtt cagtgtggcg cgcctcttca ccgtccagcc aggtcagctc
     2041 aagccccagc ggcagccagt tgtgcaggcg cttgtgcaga tgggcgaccc tccggggatt
     2101 gcgcaccacg cgcaacgccc cgcagcgcac aaagccgatg tccagcccgg ataattcctc
     2161 cagctctttc agcagatccg gcagcagagc aaagcccgcc aggacaagct cggccagagg
     2221 gccgggaccg ctcagcggac cgagcggcgc gagcaggccg gcggcggccc tggaagcgtg
     2281 tccgccaatc tcgtcccgct caaacacgct cacctccacc ctggccttgc gcagaaaata
     2341 ggcgatagcg cagcccacaa tcccgccgcc gatgatcaaa acctctgcct gcttatccat
     2401 ctcgttcctt gctagaagga cgccaaagcg ttgcctctgc tgtgcccctc gtcgtaactg
     2461 ccgcgcatct cgccgagggc actctgtttt cccctctcta ttgtgcatca caaaggacaa
     2521 ataaaacaat ggcccgcttc tggcaggcag agcttattca atgcccggct ttccctgccc
     2581 tgctccttca ggccaggggg aagatgttgc cactgggaaa gtcaccggaa gcggccctct
     2641 tgcatggaga cgttttccag ggggatcatc atgcgggatg accaataatg atgcaagaaa
     2701 atgcggacat ttgggatatt ttgccttttt gggctggtcg tggcagtgaa aaaggctcct
     2761 cgtctcgcgg gatgaggagc ctttttgtgt tgtgtcagtc tgtggacaac tggattgttg
     2821 tcgtggcgga cgggaagcgc tgcttagaaa tcgccgctgg agccaccgcc gccgaagtcg
     2881 ccgctggagc cgaaatcgcc accaaaaccg ccaccgaaat cgccgctgga gccaccgccg
     2941 aaattgtcgc ttccacctga gtagtcttcg tggcggcgtt cttcgcgctc accggcctct
     3001 ttacccagct cgtagccaag aaaaccgccg gcggcagcgc ccagaccacc ggccatccag
     3061 ggattcatgc catttctctg gggatagccg gggccgtagt tctgattata atttggcgga
     3121 taaccggcgt cataattggg attgacatag ccccggttgc cggtgaacgt gttgccgcgt
     3181 ctgcggaaca ggcgggtgaa aaggaacagc agaccgccga tgatgaccag acccaccagg
     3241 caggcgatga ctgtgcctaa cgcgctcgcg ccgctgccgc cggtctgtga ctctccgccg
     3301 ctgctactgc tggaaccggt tgagccgagc gta
//
LOCUS       DASZYN010000775         2763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_247429, whole genome shotgun sequence.
ACCESSION   DASZYN010000775 DASZYN010000000
VERSION     DASZYN010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247429"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..>2763)
                     /gene="dnaE"
                     /locus_tag="VHE33_19690"
     CDS             complement(267..>2763)
                     /gene="dnaE"
                     /locus_tag="VHE33_19690"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HVT99732.1"
                     /translation="IIKQMQYSGYFLIVWDFIKYARDHGIPVGPGRGSATGSLVAYAM
                     EITNIDPMQNVLLFERFLNPERVTMPDIDVDFCQNRRGEVIEYVTRKYGREQVAQIIT
                     FNTMAAKAAIKDVGRALDMPYGDVDRIAKLVPATIGVTIDQALKDSPPLQEAYEGTPQ
                     IKELIDTAKKLEGLVRGAGVHASAVVIAPRPLTELVPLNRTKNEEIVTAYDMKAVEKM
                     GLLKMDFLGLATLTVLTDCVKLIEQTRGEKLDFETLPLDDPETMKRVFHTALTSGIFQ
                     FESPGMRDILRRYKPDTVEDLTALNALYRPGPMDMIDDFIERKWGRRKVEFLLPELEG
                     ILKDSYGVIVYQEQVMRIANVLASYSLGEADLLRRAMGKKDPEAMAKQRDRFLSGAAA
                     LGHPKAPVNEIFDQMAKFSGYGFNKSHSAAYALVAYQTAYLKTHYPVEFMSALLTSEI
                     SKPDNVVKYIKECREMEISVEPPDVRYSDADFTPHGDKIRFGLTAIKNVGRNAIDSIL
                     TVRNELSKEGKGFAGFQEFCEKIDLRLLNKRVIESLIKAGALDSFGSRGRLLAAVDKT
                     MERAQKTQRDLEAGQAGLFGIFNAPAEGAKHDDLPNVPDMEETQRLAAEKEVLGFFVS
                     GHPLDKYAEKLRNLPGVVDIATAMEMKPAPSNGRRGQAPENEIAIAGVMVGLKVAKSK
                     RSGELYAQAALEDASGKIDLICFPKDYERLAESLRIEAPVLVRGQLRAEEEAAPKLCV
                     SAIQALEDVKVRLPMNVRIRIQLDRVSESTLEELRELVVSAPGPARVMLNVEQKGQYC
                     VVMEPEALRVAADRAFIDKAELLLGRGMVQAVD"
BASE COUNT          504 a          979 c          701 g          579 t
ORIGIN      
        1 tcacccggtc aaagaaattc accagcaccc cgatgccgag caacaccgca atgcgccagc
       61 ggcgatgcgg cgcgtgcgcg gatgccgttg gggcgttttc gctcatgagg ctttggcaat
      121 tgtagacggc gcgatggccc aacccagcgc ttcgcgtcgc ggggttgggg ttcatgcttt
      181 ggcttaggtt ctggctccgg ctcgaacgct gatcgccgat cgccgaacgc cgaacgccga
      241 acgctgaacg ctgaccgcta ttgaagctaa tccactgcct gtaccatgcc gcggcccagc
      301 agcagctccg ccttgtcgat aaacgcccga tccgccgcca cgcgcagcgc ttcaggctcc
      361 atcaccacgc agtattgtcc cttttgctcc acgttcagca tcacccgggc aggtcctggg
      421 gcggaaacca ccagctcccg cagttcctcc agcgtcgatt cgctcacgcg gtcgagctgg
      481 atgcgaatgc gcacattcat tggcagccgc acctttacgt cttccaatgc ctgaatcgcc
      541 gacacgcaca acttcggcgc cgcttcctct tccgctcgca gctgtcctcg aacgagtacc
      601 ggcgcttcaa tccgcaacga ctccgccagc cgctcgtaat ccttcggaaa acaaatcagg
      661 tcgatctttc cactggcatc ttcgagcgcc gcctgcgcgt acagttcgcc cgatcgcttc
      721 gactttgcca ccttcagccc caccatcacg ccggcaatcg caatctcatt ctccggagcc
      781 tgtccgcgcc gcccattcga cggcgccggt ttcatctcca tggctgtggc aatatccaca
      841 acgccaggca aatttcgcag cttctccgca tacttgtcca gcggatgccc cgacacgaag
      901 aatcccagca cttctttttc cgcagccagc ctctgggtct cctccatgtc cggcacattc
      961 ggcagatcgt catgtttcgc gccctctgcc ggcgcgttga aaatgccgaa cagccccgcc
     1021 tgcccagcct ccagatcgcg ctgcgttttc tgcgcgcgct ccatcgtctt gtcgactgca
     1081 gccagcagcc gcccccggct tccaaatgaa tccagtgcac ccgccttgat cagcgattca
     1141 atcacgcgct tgttcagcag ccgcagatcg atcttttcgc agaactcctg aaatccggcg
     1201 aagcccttcc cctccttcga cagctcgttg cgcaccgtca gaatcgaatc gatcgcgttc
     1261 cggcccacat tcttgatcgc ggtcagtcca aaccggatct tgtccccatg gggcgtgaag
     1321 tccgcatcgg agtaccgcac atcgggcggc tcgaccgaaa tctccatctc ccggcattct
     1381 ttgatgtact tcaccacgtt gtccggtttg gagatttccg acgtcagcag cgccgacata
     1441 aactccaccg ggtagtgcgt cttcagataa gccgtctgat acgccaccag cgcgtaggcc
     1501 gccgagtgcg acttgttgaa cccatagccg gagaacttcg ccatctggtc gaaaatctca
     1561 ttcaccggcg ccttcgggtg ccccagcgcc gccgcgccgc tcaaaaagcg atcgcgctgc
     1621 ttcgccatcg cctcaggatc ctttttgccc atcgcgcgtc gcagcagatc cgcttcgccc
     1681 agcgaatagc tggccagcac gttggcgatg cgcatcacct gttcctgata gacgatcacc
     1741 ccgtaggaat ccttgagaat gccctcgagc tccggcagca ggaactccac cttgcgccgc
     1801 ccccacttgc gctcgatgaa gtcgtcgatc atgtccatcg gccccggccg atagagcgca
     1861 ttcaaggcgg tcaaatcctc gactgtgtcc ggcttgtaac gccgcagaat gtcccgcatc
     1921 cccggggatt caaactggaa gatcccggac gtcagcgccg tgtgaaacac ccgcttcatc
     1981 gtctccggat cgtccagcgg cagcgtttcg aagtccagct tctctcccct ggtctgttcg
     2041 atcagcttca cgcagtcggt cagcaccgtc agcgttgcca ggccgaggaa gtccatcttc
     2101 agcaggccca ttttttcaac cgccttcatg tcgtaggcgg tcacgatttc ttcgttcttg
     2161 gtcctgttca gcggaaccag ttcggtcagc ggccgtggcg caatcaccac cgccgacgca
     2221 tgcacccccg cgccgcgcac cagcccctcg agcttcttcg ccgtatcgat cagctctttg
     2281 atctgcggag tcccctcgta agcctcctgc agcgggggcg aatccttcag cgcctgatcg
     2341 atggtcaccc cgatcgtcgc cggcaccagc ttcgcaatcc ggtccacgtc gccgtacggc
     2401 atatccagcg cgcgtcccac gtccttgatg gccgccttcg ctgccatcgt attgaacgtg
     2461 atgatctgcg ccacctgctc tcgtccatac ttccgcgtga cgtactcaat gacctcgccg
     2521 cgacggttct ggcagaagtc cacatcgata tccggcatcg tcacgcgctc cggattcaga
     2581 aaccgctcga acagcagcac attctgcatc ggatcgatgt tggtgatctc catcgcatag
     2641 gccaccagcg agccggtcgc cgagccgcgg ccggggccaa cgggaattcc atggtcgcgt
     2701 gcgtacttga tgaagtccca cacgatcagg aaataccccg agtactgcat ctgcttaatg
     2761 atg
//
LOCUS       DASZYN010000776         5969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_178861, whole genome shotgun sequence.
ACCESSION   DASZYN010000776 DASZYN010000000
VERSION     DASZYN010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5969
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178861"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VHE33_19695"
     CDS             complement(<1..581)
                     /locus_tag="VHE33_19695"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT99733.1"
                     /translation="MSSLQGKTALVTGASQGIGRACALALAAAGAKVALAARNETKLN
                     EVAAAIASAGGAAAVFALDIASEDSIKACAKAVIGHFGGVEILVNNAGITRDTLVLRM
                     KRADWDEVLHTNLTGTFLMTQAVLSPMLKARWGRIINISSVVGETGQAGQANYAASKA
                     GLIGLTKSLARELASRNITANAIAPGYIETAMTA"
     gene            complement(604..1395)
                     /locus_tag="VHE33_19700"
     CDS             complement(604..1395)
                     /locus_tag="VHE33_19700"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HVT99734.1"
                     /translation="MNNILSTTEHRPWPLPKSPWVMTQRWNDLLFAHWPLPASDLTNL
                     LPESLTVDTFDGTGWVGVVPFWMDQIRMRRFPQMPGANRFPELNLRTYVRERHTNQPG
                     VYFFSLDAANPFAVSAARLFFRLPYYWARMKVESDSEHRIHYSSDRMLSKRPAHFRAR
                     YRSLGKPCVKGGMESFLTERYALFTASRGGQLSRVNIHHLPWPLELAEAEFEVNDLPQ
                     AHGIQLPDTKPLLQYSRELVVYIWAVESLGSPATRRTAAPVPAGP"
     gene            1563..3155
                     /locus_tag="VHE33_19705"
     CDS             1563..3155
                     /locus_tag="VHE33_19705"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HVT99735.1"
                     /translation="MASMTETPPAKTETPVTPESRNNQTDVFAVHGADPEVLAYAMAK
                     YSRSALSMKESLREISSQKAEQFLNTFYFQYGHRSIADLAHVAFAVERLSLLAAIVLV
                     DEQRWDGQERSTRYQNFRKSGWYMPDFGADSASTNRYRETVEQLFGTYHSVFEAVFVS
                     LQSQVPKPADMKQESYDRTLKARAFDVARYLLPLATNTSLGQIVNARTLETQVSRLLS
                     HPVAEVRQLGVRLQAAASGAAWNVYADDLAALQKDIAALDPALGERAAALLTREVKTA
                     PTLVKYAAVNDYQLQTRAELREAAAELLRGEPIEPAPLVDLIDDADTLEIDLATTLVY
                     GQSHYPYRQIRRHIAALSDARRNEIVDLGIRHRGRHDELLREFAAGQSLRFDILMDIG
                     GFRDMHRHRRCVQILQGFTSAHGYAIPEGVPEAGVQTLYEDTMRAAHAAYSTLAAGSA
                     PEAAESAQYVLPLGTKNRALFKMDFAEALYISELRSAPQGHFSYRRVAWEMFRAVERK
                     HPALAKYFRVTNADEPIDLLKR"
     gene            complement(3225..3572)
                     /locus_tag="VHE33_19710"
     CDS             complement(3225..3572)
                     /locus_tag="VHE33_19710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99736.1"
                     /translation="MRRTLTAPLVLLTIAALLGAPGCEDPPTQIRNCVDDNRHIVPDD
                     VCDNQPFTGSEILPGSYHYLYDGASGGHTGDVVVGGSWQPKFGARIVSGDTGATIHTV
                     NELPHGGEGLGGD"
     gene            3556..5829
                     /locus_tag="VHE33_19715"
     CDS             3556..5829
                     /locus_tag="VHE33_19715"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HVT99737.1"
                     /translation="MSVLRMVWSSALIFADAAYKRNRSLIGCLHRRLPSDTMQLHPED
                     IPVYRKTLLSFACILFATLQPMTTPAANAQNTSFGSENPFYAPSTLPFHAPPFDRIKD
                     SDYQPAIEAGIAEQQRDMDAIAGNSAAPTFENTFVAMEKTGQLLYRAQAAFNAVTGAN
                     TNPYLQNVQRELAPKLAAHHDAIYLNQKLFARVRTVYEERQRLHLDAESQRLVKIVYD
                     EFVHSGAALSDSDREKLKKLNEEASKLEDEFSRGLLDATKKAAFTTTDKSALAGLTDA
                     QIAAAAQAAEARKSQGYVIPLQNTTQQPDLTELTNRGTREALFQKSWTRAEQPGNDLR
                     PVVARLAQLRAEKAKLLGYETYAAWKLEDQMAKTPDAVLHFLNELIPAATAKAQAEGK
                     EIQNVIDAQHGGFQLQPWDWNFYAEQVRKAKYDLDESQVKPYFEINNVLENGVFYAAH
                     LLYGLSFTERKDIPVWQPDVRVFEVHDAVGKPLALFYCDYFKRDNKNGGAWMSSFVDQ
                     SRLLGNLPVVYNVANLPKPAPGQPALISSDDVRTMFHEFGHALHGMLSNVEYPTLSGT
                     SVPRDFVEFPSQFNEHWATYPAVFQHYAKHYQTGQPMPPDLAAKILKARDFNQGYALT
                     ELLAASMLDMQWHTLPADAPLQNVDQFEQQALEKSRVAIPTVPPRYRSTYFLHIWAND
                     YAAGYYAYQWTEMLDDDAYQWFEEHGGLTRANGDRFRRMVLSRGNTENLSDLYAAWRG
                     RAPEIGAMLKDRGLENK"
BASE COUNT         1191 a         1970 c         1700 g         1108 t
ORIGIN      
        1 gcggtcatag cggtttcgat gtagccggga gcaatggcgt tggcggtgat gttgcgcgaa
       61 gccagctccc gcgccagcga tttggtcagt ccgatcaacc ccgccttgct ggcggcgtag
      121 ttcgcctgac ccgcctggcc cgtttctcca accacactcg aaatgttgat gatccgcccc
      181 cagcgcgcct tcagcatcgg actgagaacc gcctgcgtca tcagaaacgt gccggtgaga
      241 ttcgtgtgca gcacctcgtc ccagtccgcg cgtttcattc gcagcaccag cgtatcgcgc
      301 gtgatgccgg cattattgac cagaatctca acgccgccaa agtgtccaat cacggccttc
      361 gcacaggctt tgatcgagtc ttcactggca atatcgagcg caaacaccgc cgccgctccg
      421 cctgctgagg cgatggcagc agcgacctca ttcagtttgg tttcattcct tgccgccagc
      481 gcaacctttg ctccggcggc agccagcgcc agagcgcagg cccgaccgat tccctgagat
      541 gctcctgtga ccagagctgt ctttccctgc aggcttgaca tggttccagc ctatcgcatc
      601 gattcacggc ccggctggaa ccggtgccgc cgtacgccgt gtcgccggcg atccaaggct
      661 ttccaccgcc cagatgtaga ccaccagctc gcggctgtac tgcagcagag gcttcgtatc
      721 cggcagctga atgccgtggg cctggggaag atcgttgact tcgaactccg cttcggccag
      781 ttccaaaggc catggcaggt gatgaatgtt cacacgggaa agctgccccc cgcggcttgc
      841 cgtgaaaagt gcatagcgtt cggtcagaaa gctttccatg ccgcccttga cgcagggctt
      901 gccgagactt cgatagcgcg cacgaaaatg cgccggccgc ttgctgagca tgcgatcgct
      961 gctgtagtga atcctgtgct cgctgtccga ctccactttc atccgcgccc agtagtacgg
     1021 aagccggaag aacagccgcg ccgcagaaac agcaaaggga ttggcagcat cgagcgaaaa
     1081 gaagtaaacc ccgggctgat tcgtatgccg ttcacgcacg taggtccgca ggttgagctc
     1141 tggaaagcga ttcgctcccg gcatctgcgg aaaacgccgc atgcgaatct gatccatcca
     1201 gaagggaacc actccgaccc aaccggtacc gtcgaacgta tccacagtca gtgattcagg
     1261 gagcaggttg gtcagatcac tcgccggcag cggccagtgg gcaaacagca aatcgttcca
     1321 gcgctgcgtc atcacccacg gcgacttggg aaggggccac ggacggtgct cggtcgtcga
     1381 gaggatgttg ttcatgcact agggtcgtcc ccggcggcta ctttgtcagc gatttttcca
     1441 cgccgaaaac actctcatgg tactcgttct ttctttgcag tcacataacg atgtggccaa
     1501 ccgccgggtg acttcctgga tgcatgcggg atttccgcat ctccggggtt tagcattcct
     1561 gcatggcttc catgaccgag actcctccag caaaaacaga gacgccggtg acgcccgaaa
     1621 gccgcaacaa ccagaccgac gtgtttgccg tccacggggc cgatcccgaa gtgctcgcct
     1681 atgccatggc gaagtactcc cggtcggcgc tctcgatgaa ggagtcgctg cgcgaaatca
     1741 gcagccagaa ggccgagcag tttctcaaca ccttctactt ccagtacggt catcgctcga
     1801 tcgcggatct cgcccacgtc gcattcgctg tggaacggct gtccctgctg gctgcaatcg
     1861 ttttggtgga tgagcagcgc tgggacgggc aggaacgatc cacgcgctac cagaacttcc
     1921 gcaagagcgg ctggtacatg ccggatttcg gtgccgattc cgcctccacg aatcgctatc
     1981 gcgaaaccgt tgagcagctc tttgggacct atcacagcgt ttttgaggcc gttttcgtct
     2041 cgctgcaaag ccaggtaccg aagccggccg acatgaagca ggagagctac gaccggacgc
     2101 tcaaagcccg tgctttcgat gtggctcgtt atctgttgcc cctggcgacc aacacgtcac
     2161 tggggcagat cgtcaacgcc cgcacgcttg aaacccaggt ttcgcgcctg ctttcccatc
     2221 cggttgccga ggtgcgccag ctgggggtgc gtctgcaggc cgccgcttcc ggcgccgcct
     2281 ggaacgtcta tgccgacgac ctggctgcgc ttcagaagga cattgcagcg ctcgacccgg
     2341 cgctcggcga acgcgccgcc gctctgctga cccgtgaagt gaagaccgcg ccgacgttgg
     2401 tcaaatatgc cgcggtcaat gactatcagc tgcagacccg ggcggagctg cgcgaggccg
     2461 cagccgagct gttgcggggc gagccgatcg agcccgcgcc gctggtcgac ctgatcgacg
     2521 atgcggacac gctcgagatc gatctcgcca cgaccctggt ttacggccag agccattacc
     2581 cgtaccggca gatccgccgc cacatcgccg ctctcagtga tgcccgccgc aacgagatcg
     2641 tcgacctcgg catccgccac cgcgggcgtc acgacgaact cctgcgggag ttcgccgcgg
     2701 gacagtcgct ccgcttcgac atcctcatgg acatcggcgg cttccgcgac atgcatcgcc
     2761 accggcgctg cgtccagatt ctgcagggat tcacttcggc ccacggctat gcaattcccg
     2821 aaggcgtgcc ggaagccggc gtgcagactc tctacgagga cacaatgcgg gccgcacatg
     2881 ccgcttactc cactctcgcc gctggttcag cgcccgaagc ggccgagtcc gcccaatatg
     2941 tcctgcccct cggaacgaaa aatcgcgcgc tcttcaagat ggatttcgcc gaagcgctct
     3001 atatctccga gcttcggtcc gccccgcagg ggcacttctc ctatcgccgc gtcgcctggg
     3061 agatgtttcg tgcggtcgag cgcaaacatc cggccctggc aaaatatttc cgcgtcacga
     3121 acgccgacga gcccatcgac ctgctgaagc gctgaagcgt gcagcgcgag ccgatcgcac
     3181 tgcgccgaag gcgcgtatgc atggacggcg agtccccagg acggctagtc cccaccaagg
     3241 ccctcaccgc catgggggag ttcgttgacc gtgtggatcg tcgctcccgt atcgccggag
     3301 acgatgcgcg ccccgaattt cggctgccac gatccgccca ccacgacgtc gccggtatgg
     3361 ccgccgctcg caccgtcgta gaggtagtgg tagctgccgg gcagaatctc gctcccggtg
     3421 aacggctgat tgtcgcaaac atcgtccgga acaatgtggc ggttgtcgtc gacgcagttg
     3481 cgaatctgcg tgggagggtc ctcgcatcct ggagcgccca aaagggcagc aatggtaagc
     3541 aggacgagag gcgccgtgag tgttctccgc atggtctggt cttcggcgct tatcttcgcg
     3601 gatgctgcct acaaacgcaa ccgcagtctc attggctgtc tgcaccgccg cctgccgagc
     3661 gatacaatgc agcttcaccc tgaggacatt cccgtgtacc ggaagactct gctttctttc
     3721 gcctgcattc ttttcgcgac ccttcagccc atgaccacac ccgccgcaaa cgcacagaac
     3781 acatcttttg gctcggagaa tccgttctac gctcccagca cgctgccgtt ccacgcaccc
     3841 ccctttgatc ggatcaagga ttccgactac cagcccgcca tcgaggccgg catcgccgag
     3901 cagcagcgag acatggacgc catcgccggc aactccgccg cgcccacgtt cgagaacact
     3961 tttgtcgcga tggagaaaac cggccagctc ctctatcgag cccaggccgc cttcaacgcg
     4021 gtcaccggcg ccaacaccaa tccatatttg cagaacgtgc agcgcgagct tgcgccgaaa
     4081 ctcgctgccc accatgatgc gatctatctc aaccaaaaac tcttcgcccg agtacggacc
     4141 gtttatgaag agcgccagag actgcatctc gatgccgaga gtcagcgcct ggtaaagatc
     4201 gtttacgacg aatttgtcca ctctggcgcg gccctcagcg acagcgaccg ggagaaactg
     4261 aagaagctga acgaggaagc ctcgaagctc gaggacgagt tcagccgtgg gcttctggac
     4321 gccacgaaga aagccgcctt caccacgacc gacaagtcgg cccttgccgg cctgacggac
     4381 gcccagattg ccgcagccgc tcaggccgcg gaagcccgta aatcccaggg ctatgtgatc
     4441 ccactccaga acacgacgca gcagcccgat ttgaccgaac tcaccaaccg cggcacgcgc
     4501 gaggctctct tccagaagag ctggactcgc gccgagcagc cgggcaacga cctgcgtccc
     4561 gttgtcgccc gcctggcgca gcttcgcgcg gaaaaggcga agctgctggg ctacgagacc
     4621 tacgccgcgt ggaaactcga agaccagatg gcgaagactc ccgatgccgt cctgcacttc
     4681 ctcaacgaac tgatccctgc cgcgaccgcg aaggcccagg ccgagggcaa ggagatccaa
     4741 aacgtcatcg acgcgcagca tggcggcttt cagctccagc catgggactg gaatttctac
     4801 gccgagcagg ttcgcaaggc aaagtacgat ctcgatgaat cgcaggtgaa gccgtatttc
     4861 gagatcaaca atgtcctcga gaacggcgtc ttctacgcgg ctcaccttct ctacggcctc
     4921 tccttcaccg aacgcaagga cattcccgta tggcaaccgg atgtgcgtgt cttcgaagtg
     4981 catgatgccg ttggaaagcc gcttgccctt ttctactgcg actacttcaa gcgcgacaac
     5041 aagaatggcg gcgcgtggat gtcgagcttc gtcgaccaga gccgtctgct cggcaatttg
     5101 cccgtcgttt acaacgtcgc gaacctgccc aagccggctc cgggacagcc cgctctcatc
     5161 agctccgacg atgtccgcac catgtttcac gaattcggcc acgcgctgca cggaatgttg
     5221 agcaacgtcg aatacccaac gctctccggc acgtcggttc cgcgcgattt cgtcgagttc
     5281 ccctcgcagt tcaacgaaca ctgggccacg tacccggcag tctttcagca ctatgcgaag
     5341 cactaccaaa ccggccagcc catgccgcct gatttagcgg caaagatcct caaggcccgc
     5401 gacttcaacc agggctatgc gctcacggaa cttctcgcag cgtcgatgct cgatatgcag
     5461 tggcacacac tgcccgccga tgcacctctg cagaatgtcg atcagttcga gcagcaggcc
     5521 ctggagaaat cgcgcgtggc gatcccgacc gttccgccgc ggtatcgctc cacctatttc
     5581 ctgcacatct gggcgaatga ctacgcggcg ggctactacg cctatcagtg gacggagatg
     5641 ctcgacgacg acgcgtatca gtggttcgag gagcatggcg gcctgacgcg agccaacggc
     5701 gatcgcttcc gcaggatggt tctctcgcgc gggaacacag aaaatctttc cgacctctac
     5761 gctgcgtggc gggggcgcgc accggaaatc ggagccatgc tcaaagaccg cgggctcgag
     5821 aacaaatagc gccgatcgtc ctcaaacacg aagggctgcc ctcgggcagc cccttttcac
     5881 agaagccggc aaaaattcag gcgcgagcgt aggacgcgat ggcgctcgcc tcgtcgtcct
     5941 tcacgtcgaa cacggtatac agcttggtg
//
LOCUS       DASZYN010000777         3802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_876509, whole genome shotgun sequence.
ACCESSION   DASZYN010000777 DASZYN010000000
VERSION     DASZYN010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876509"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1974)
                     /locus_tag="VHE33_19720"
     CDS             complement(<1..1974)
                     /locus_tag="VHE33_19720"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HVT99738.1"
                     /translation="MMEFHIARAVRDRYQVSETLFNFVGNVIFADLTSCRELAYRMTE
                     ARTAAGLDPIHPGALFAMGLIDEISHAIVARYRQSRDPKVSQAALQWFSARVGDDNVE
                     RLLRTFVQEFPNVAIYRGDVSIERWLDRSTEGLQHREAVLEELMLLWLANQNPAFRPF
                     RELYDDSKLAQSTAYSQVTAQLGSYFATRSETGMGRGNLLDLLRAPMEAAPDSLSGQL
                     AWIRENWGDYLGESFRKILLATDVLKEEEVAIWMRFHPTDAQRWHAPLSDTHHSEVPD
                     FRATTHPGDVEYERFSPDQEWMPNVVMMAKSTYVWLAQLTRQYGRPIERLDQVPDEEL
                     QTLANRGFNALWLIGLWERSRASQRIKHMRGQQDAVASAYSLFDYRIADDLGGDHAYR
                     NLRDRAARFGIRLASDMVPNHMGIDSPWVVEHPEWFLSRPDRPWPSYQFNGPDLSPDG
                     RVEIKIEDHYYDQSDAAVVFQRRDNGSGHTQYVYHGNDGTSFPWNDTAQLNYLRADVR
                     EQVIQTILHVARLFPIIRFDAAMTLARRHVQRLWFPLPGSGGSIPSRAEYSMTQEEFD
                     SAMPNEFWREVVDRVAAEVPGTLLLAEAFWLLEGYFVRTLGMHRVYNSAFMNMLRDEE
                     NAKYRSVIKNTVEFDPDILKRYVNFMSNPDERTA"
     gene            2072..2971
                     /locus_tag="VHE33_19725"
     CDS             2072..2971
                     /locus_tag="VHE33_19725"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HVT99739.1"
                     /translation="MPELPDITAYLTALQKRITGQKLEKIRLASFTLLRTVEPRLESA
                     EGRTVRELRRIGKRIAIGLENDVWLVLHLMIAGRLHWKSPGAKLAGRNALAAFDFSSG
                     TLLLTEAGSKRRASLYVVAGEEGLLGLDPGGIDVLGATPLQFRAALTAENRTLKRALT
                     DPRLLSGIGNAYSDEILHVARLSPVMLTHRMKDEEWARLYEATQTTLRLWIDRLEKES
                     REGFPEKVTAFRPEMAVHGKFGQPCPRCGEKIQRIRYADNETDYCAKCQTGGKLLADR
                     GLSRLLKQDWPKTLDELEALKRR"
     gene            3114..>3802
                     /locus_tag="VHE33_19730"
     CDS             3114..>3802
                     /locus_tag="VHE33_19730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99740.1"
                     /translation="MKKIGVLFGMENTFPGALVERINSRNIDGITAEFVNIGAIRLDK
                     PPAWSVIVDRISHDIPFYRAFLKHAVLHGTTVINNPFWWSADDKFFNYSLAAKLDVAA
                     PPTVILPHKHYPPGTTDKSMRNLQFPLDWDGVFSYVGFPAFLKPVNGGGWRDVHHVHS
                     REEFFQAYDQSRDLCMMLQRGVKFTEYFRCYVVGQEKVRIMYYDPRRPHAERYVKNPK
                     PAEKKLLDKVEA"
BASE COUNT          753 a         1108 c         1225 g          716 t
ORIGIN      
        1 cgccgtgcgc tcatccggat tgctcatgaa gttcacgtaa cgcttgagga tgtccggatc
       61 gaactcgacc gtattcttga tcaccgaccg gtacttcgcg ttctcctcgt cgcgcagcat
      121 gttcatgaac gcgctgttgt aaacccggtg catgcccagc gtccgcacaa agtacccttc
      181 gagaagccaa aaggcctcag caaggagcaa agtgccgggg acttcggcgg caacgcgatc
      241 gacgacctcg cgccagaact cgtttggcat ggcgctgtcg aattcttcct gcgtcatcga
      301 gtattcggcg cgcgagggaa tggatccgcc gctgccgggc agcgggaacc acaagcgctg
      361 cacgtggcgg cgcgccaggg tcatggccgc gtcgaatcgg atgatgggga acagccgcgc
      421 gacgtgaagg atggtctgaa tgacctgctc gcggacatcc gcacgcaggt agttcagctg
      481 cgcggtgtcg ttccagggaa agctggtccc gtcattgccg tggtagacgt actgcgtgtg
      541 gcctgagccg ttgtcgcggc gctggaagac gacggcagcg tcggactgat cgtagtagtg
      601 gtcctcgatt ttgatttcga cacggccgtc gggcgagagg tcaggaccgt tgaactgata
      661 cgaaggccag ggccggtcgg ggcgcgagag gaaccactcc ggatgctcga ccacccaggg
      721 cgagtcgatg cccatgtgat tcggcaccat gtccgacgcc agccgaatgc cgaagcgcgc
      781 ggcgcgatcg cgcaggttgc ggtaggcatg atcgccaccg aggtcatcgg caatgcggta
      841 atcgaagagc gaataagcag aggccacagc atcctgctga ccgcgcatgt gcttgatgcg
      901 ctgtgaagcg cgcgagcgtt cccacaggcc gatcagccac agcgcgttga agccccggtt
      961 cgcaagggtc tggagttctt cgtcgggaac ctgatcgagt cgctcaatgg gccgcccata
     1021 ctggcgcgtc agctgcgcga gccacacgta ggtgctcttg gccatcatca ccacgttcgg
     1081 catccactcc tggtccgggc tgaagcgctc gtattccacg tcgccgggat gcgtggtggc
     1141 gcggaagtcg ggtacctccg aatggtgcgt gtcactgaga ggagcgtgcc agcgctgcgc
     1201 atccgtggga tggaagcgca tccagatggc gacctcttcc tctttcagaa cgtcggtggc
     1261 gagaagaatc ttcctgaagc tttcgccgag atagtccccc cagttctcgc gaatccaggc
     1321 gagttgcccg ctcagtgaat cgggtgccgc ttccatcggt gcgcgcagaa ggtcgagcaa
     1381 attgccccgg cccatacccg tttccgaacg tgtggcgaaa tagctgccca gctgtgcggt
     1441 gacctggctg taggccgtgc tctgggccag cttgctgtcg tcgtagagtt cgcgaaacgg
     1501 ccggaaggcg gggttttggt tcgccagcca cagcagcatc agctcttcca gaacggcttc
     1561 gcgatgctgc aggccttccg tcgaccggtc gagccagcgt tcgatggaca cgtcgccgcg
     1621 atagatggcg acgttgggga attcctgaac aaaggtgcgc agcaggcgct cgacattgtc
     1681 gtcgcccacc cgcgccgaga accactgcag cgcagcctgg ctcacctttg ggtcgcggct
     1741 ctggcggtac cgcgccacga tggcgtggct gatctcgtcg atcaggccca tagcaaagag
     1801 cgcaccggga tggatcggat cgaggccggc cgcggtgcgc gcctccgtca tgcggtaggc
     1861 gagttcacga caggaggtca gatcggcgaa gatgacgttg ccgacgaaat tgaagagggt
     1921 ctcgctgacc tggtagcggt cgcgaaccgc gcgcgcaatg tggaattcca tcatgtgatt
     1981 ctgttagacg caggttgagg ttgcgccttt gccagcgggt ttggattcgc gttgaaggga
     2041 accactggga tgcgagatac catacgaatc gatgcccgaa ttgcccgaca tcacagcgta
     2101 cctgaccgcg ctgcaaaagc gcattaccgg ccagaagctc gaaaagattc gcctggccag
     2161 ctttacgttg ttgcgcaccg tagaaccgcg actggaaagc gctgaagggc gcacagtccg
     2221 cgaactgcgg cggatcggaa agcgcatagc aattggcctc gagaacgatg tttggctcgt
     2281 gctgcacctc atgattgccg gccgcctgca ctggaaatca ccaggggcga agctggccgg
     2341 tcgaaatgcc ctggccgcct tcgatttttc gtcgggaacc ctcctgctga ccgaagcggg
     2401 gtcgaaacgc cgggcgtcct tgtatgtcgt ggcaggggaa gaggggcttc tcgggctcga
     2461 tcccggaggc atcgacgttc tgggcgccac gccgctgcag tttcgcgcgg cactgacggc
     2521 tgagaatcgg acgctgaagc gcgcgctgac cgatcctcgc cttctcagcg gaattggcaa
     2581 cgcatactcg gacgaaatcc tgcatgtggc ccggctttcg cccgtgatgc tcacgcatcg
     2641 catgaaggat gaggagtggg cgcggctgta tgaggcgacc caaaccacgc tgcgcttgtg
     2701 gatcgaccgg ctcgaaaagg agagtcgcga aggctttccg gaaaaggtga ccgcgtttcg
     2761 gcccgagatg gcagtgcacg gaaaatttgg ccagccgtgc ccgcgctgcg gcgagaaaat
     2821 tcagcggatc cgctacgcgg acaacgaaac ggactactgc gcaaaatgcc agacgggcgg
     2881 aaagctcctc gccgatcgcg gattgtcccg cctgctcaaa caggactggc caaaaaccct
     2941 cgacgagttg gaagcgctga agcggcgata gctgaaccgg ttgagtgtcc tgatattgac
     3001 tcttttgcaa cgattgcgaa ggtcggctaa ggttgagtca tactcctgtg caggcgcgtg
     3061 ccgagcgagc ccggacgcgt attcccccgg ctaacaacct gagaggcgga aacgtgaaaa
     3121 agatcggtgt gcttttcggt atggaaaata cgttccccgg cgccctcgtg gagaggatca
     3181 acagcaggaa catcgacgga atcaccgcgg agtttgtgaa cattggggcg atccggctcg
     3241 ataaaccgcc ggcgtggtcg gtcatcgtgg atcgcatctc gcacgatatt ccgttctatc
     3301 gggcgttcct gaagcacgcg gtcctgcacg gcacgaccgt gatcaacaac cccttctggt
     3361 ggtcggcgga cgacaaattc ttcaactact cgctggcagc aaagctggac gtcgccgcgc
     3421 cgccgaccgt gattctgccc cacaagcact atccccccgg cacgacagac aagtcgatgc
     3481 gcaacctgca gtttccgctc gactgggatg gagtcttttc ttatgtcggg ttcccggcgt
     3541 ttctgaagcc ggtgaatggc ggcggctggc gcgatgtcca tcacgtccat tcgcgcgagg
     3601 agttcttcca ggcctacgac cagagccgcg acctgtgcat gatgctgcag cggggcgtga
     3661 agttcaccga gtattttcgt tgctatgtgg tcgggcagga gaaggtgcgg atcatgtact
     3721 acgatccgcg gcggccgcat gccgagcggt acgtaaaaaa tccgaagccc gcggagaaga
     3781 agctgctcga caaggtagaa gc
//
LOCUS       DASZYN010000778         3544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_54505, whole genome shotgun sequence.
ACCESSION   DASZYN010000778 DASZYN010000000
VERSION     DASZYN010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54505"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VHE33_19735"
     CDS             complement(<1..482)
                     /locus_tag="VHE33_19735"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HVT99741.1"
                     /translation="MAAASATVASALLLSCAASNTIDFLYVTSNKVNPGQINVYEVNS
                     ESGALFQIADSPYPSGGRNPVDEVTSPNGKNLYVVNHDDNTIVQFAIGTDGKIYPLQT
                     VNTPGTEPVALALNADGTALYVLDYYGPSAPGHPSYTDLNPGPGALVAYPISIDSKSG
                     L"
     gene            complement(533..1879)
                     /locus_tag="VHE33_19740"
     CDS             complement(533..1879)
                     /locus_tag="VHE33_19740"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HVT99742.1"
                     /translation="MKFSKLGRIALATAASLFIGFGTQSCYYYTEAYIIVTGSQYNQV
                     ASYREQNQTGKLTQAPHSPLSSGGNNPIRALLLNGGRYVYVLNQGTPKTNADGSITWT
                     GASIAQFSIGGDGSLAFQQNYFSQGRGSLRLALSTAGNYLYVLDQYQPLGNDANTTPA
                     SPSVSASTPCYDASAGVYRPAGDITVFSIDSNTGRLFLVQNQQQQNSFGTPLTYFPLG
                     CGAIDFHFLANYLYTAENSDPANPGISGQVIYAYSVNPSNGQLTQVPGGSQPVPGATN
                     IAVLGTSASGTYLYVLDNGTDQIFTFTNSGGNGLLSAISTGAAFNAPNTSGMTALTTD
                     SNSKYLYVANTQSTSLGSPKSVISAFDITQSSGALTLFNVNPANNTATFGVGTTPVCI
                     FEDPSHQYIYTANAGSSDITGSKLDPNTGTLTNLSKGSTFATVGTPTWCLYSSNTN"
     gene            complement(2090..>3544)
                     /locus_tag="VHE33_19745"
     CDS             complement(2090..>3544)
                     /locus_tag="VHE33_19745"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HVT99743.1"
                     /translation="EIRAKYHDYIVAVLKLTGESEDQAKADADHVMEIETALAKGSTP
                     RVDLRDPNKRYHIMTLTDLDQLEPSFDWSAYFAGAGAPKVADLNVGQPDFFKTENQVI
                     GDQSLDALKAYLKFHVLNGVAPWLSQPFEDASFDFFQKTLQGQAEQTARWKRCTAATD
                     RALGEAVGQDWVKENFPPQAKQNMEQLVAALKTALGKDIQALPWMSEETKKQAEEKLA
                     AFRQKIGYPDKWRDYSKFEVTRSNFVEDLNHSAAFEFDYEMNKVGKPVDEKEWGMTPP
                     TVNAYYNPPQNDINFPAGILQPPFYDFSKDPAVNFGGIGVVIGHEMTHGFDDQGSKYD
                     GQGNVREWWTPEDRSKFEERTDCEVKEYGSFEPVPGVKLNGKLTLGENTADNGGIRIA
                     WQALLATLAKEGKSIDDKIDGYTGAQRYFISFAQVWCENRTEQVSRVAAKTDPHSPGR
                     FRANGTVQNFDEFGKAFGCHVGQPMMPENACRVW"
BASE COUNT          626 a         1036 c         1119 g          763 t
ORIGIN      
        1 agcccggatt tcgaatcgat ggagatgggg taagcgacca gcgcgccggg gccgggattc
       61 aggtcggtgt aagaaggatg acccggagcc gagggcccat agtagtcgag cacatacagt
      121 gccgtgccgt ccgcattcag cgcgagtgcg accggctcgg tgccgggagt gttcacggtc
      181 tgcagcggat agatcttgcc gtcggtgccg atcgcgaact gcacgatggt gttgtcgtcg
      241 tgattgacca cgtaaagatt cttgccgttc ggcgaggtca cttcatcgac tggattgcgg
      301 ccgcccgaag gatacggcga gtcggcgatc tggaacagag cgccggactc ggagttcacc
      361 tcgtaaacat tgatctggcc agggttcacc ttgttactgg tgacgtagag aaaatcgatc
      421 gtattgctgg cggcgcacga aagcagcagc gccgacgcga cggttgccga cgcggcggcc
      481 acaagccgcc tgccgatttt ctgccatttc tgttgcctga atcctgctcg catcagtttg
      541 tattggaact gtagaggcac caggtcggag ttcccacggt cgcgaatgtc gagcctttcg
      601 agagattcgt gagggtgccg gtattgggat cgagtttcga gcccgtgata tcgctggagc
      661 ccgcgttggc ggtgtagatg tactggtgcg acggatcctc gaagatgcat accggcgtcg
      721 tgcccacccc gaaggttgcg gtgttattcg ccggattcac attgaacagg gtcagagcgc
      781 ccgaactctg cgtgatgtcg aatgccgaaa tcacgctctt gggtgagcca aggctggtcg
      841 actgcgtgtt cgcgacgtac aggtacttcg agttgctgtc ggtggtcagc gcggtcatgc
      901 ccgaggtatt cggcgcattg aaggcggctc cggtggagat ggccgacaac aggccgttgc
      961 cgccactgtt ggtgaacgta aagatctggt cggtcccgtt gtccagcaca tagaggtagg
     1021 tgccgctggc gctggtgccg aggaccgcga tattggtcgc gcccggcacc ggctgcgaac
     1081 cgcccggtac ctgtgtcagc tggccgttcg atgggttcac gctgtacgcg tagatgacct
     1141 gcccgctgat gcccggattc gccggatcgc tgttttcggc cgtgtacaga tagttggcca
     1201 ggaagtggaa atcgatcgcg ccgcagccca ggggaaagta cgtgagcggc gtcccaaagg
     1261 aattctgctg ctgctgattc tggacgagga agaggcgtcc cgtgttgctg tcgatgctga
     1321 agaccgtgat atcgccggcc gggcgataaa cgccggcaga cgcgtcgtag cacggcgtcg
     1381 acgcgctcac cgatggcgat gccggcgtcg tgttcgcatc gttgcccagc ggctggtact
     1441 ggtcgagcac gtagaggtaa ttcccggcgg tcgacagcgc gagtcgcaga gatccgcggc
     1501 cctggctgaa gtagttctgc tggaaggcca gtgatccgtc gccgccgatg ctgaactgag
     1561 cgatgctggc gccggtccac gtaatggatc cgtccgcatt ggtcttgggc gtgccctggt
     1621 tgagcacgta gacgtaacgc ccgccgttga gcaagagagc gcggatggga ttgttgcccc
     1681 cggaactgag cggactgtgc ggagcctgag tcagcttgcc ggtctggttc tgttcccggt
     1741 aggacgccac ctggttgtac tgcgatccgg tgacgatgat gtaggcctca gtgtagtagt
     1801 agcaggactg ggtaccgaac ccgatgaaca gggatgccgc cgtggccagg gcaatccggc
     1861 cgagtttgct aaacttcatg cgcttccttc acgccggttc gctgagggag cgagcccgga
     1921 caaagctgta tggatgatcc cgaaccattg taaatggaac ggggatgttg cgccgcgtca
     1981 gccaggccga aacaggcgcg ggggacggcc ctgcttcggt atggacatta gacgccgtaa
     2041 cgcgcgggag atcaccaggc ggaatggctc acaaaattac cggacaactt caccaaactc
     2101 ggcatgcgtt ttccggcatc atcggctggc cgacatggca gccgaaggct ttgccgaact
     2161 cgtcaaaatt ttgtacggtc ccgttggcgc ggaagcggcc cggtgaatgc ggatcggtct
     2221 tcgccgccac ccgggagacc tgctcggtgc gattctcaca ccacacctgg gcaaagctga
     2281 tgaagtagcg ctgcgccccg gtgtagccgt cgatcttgtc gtcaatgctc ttgccctctt
     2341 tggccagcgt cgcgagcagc gcctgccatg cgatgcgaat cccgccgtta tcggccgtgt
     2401 tttcacccag ggtgagcttg ccgttgagtt tcacgcccgg cactggctca aagctgccgt
     2461 actctttcac ctcgcagtcc gtccgctctt cgaacttgct gcgatcctcc ggcgtccacc
     2521 actcgcgcac attcccctgg ccgtcgtatt tgctgccctg gtcgtcgaac ccgtgcgtca
     2581 tctcgtgccc gatgaccacg ccgatgcctc caaaattcac cgccgggtct ttgctgaagt
     2641 catagaaagg cggctgcaga atgcccgccg ggaagttgat gtcgttctgc ggcggattgt
     2701 agtaggcgtt cacggtggga ggcgtcatgc cccattcctt ctcgtccacc ggcttgccca
     2761 ccttgttcat ttcgtagtcg aactcaaaag ccgccgagtg gttgaggtct tcgacaaagt
     2821 tcgagcgcgt gacctcgaac ttcgaatagt cccgccactt gtcgggatag ccgatcttct
     2881 gccggaacgc cgcgagcttt tcttcagcct gctttttggt ctcctcgctc atccacggca
     2941 gcgcctgaat gtcttttccg agtgcagtct tcagagcggc gaccagctgc tccatgttct
     3001 gtttcgcctg gggcgggaaa ttctctttca cccagtcctg gcccaccgct tcgccgaggg
     3061 cgcgatccgt cgctgcggtg cagcgcttcc agcgcgcggt ctgttccgcc tggccctgca
     3121 gcgtcttctg gaagaagtcg aaactggcat cctcgaaagg ctgcgagagc cagggagcga
     3181 cgccgttcaa aacgtggaat ttcaaatagg ctttcagcgc gtcgaggctc tgatcgccga
     3241 tgacctggtt ttccgtcttg aagaagtccg gctgcccgac attcagatcg gccactttcg
     3301 gagcgccggc tccggcaaaa tacgcgctcc agtcgaagct gggctccagc tgatccagat
     3361 ccgtcagcgt catgatgtgg tagcgcttgt tgggatcgcg cagatcgacc cgaggcgtcg
     3421 agcccttcgc cagcgccgtc tcgatttcca tcacgtgatc ggcgtccgcc ttcgcctgat
     3481 cctccgattc gccggtgagc ttcagcaccg ccacgatgta gtcgtgatat ttggcgcgga
     3541 tctc
//
LOCUS       DASZYN010000779        19324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_748526, whole genome shotgun sequence.
ACCESSION   DASZYN010000779 DASZYN010000000
VERSION     DASZYN010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 19324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19324
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748526"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(401..1630)
                     /gene="glgA"
                     /locus_tag="VHE33_19750"
     CDS             complement(401..1630)
                     /gene="glgA"
                     /locus_tag="VHE33_19750"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HVT99744.1"
                     /translation="MRVGLMTREYPPNVYGGAGVHVEYLSRELAKKIEVEVHCWGDQN
                     SDQGNLQVRGEQPWSRISQGTEEKFKTALETFSLNLAQMTSIGSIDIVHTHTWYVAMA
                     GFLAKKLYKIPFVLTTHSLEPLRAWKSEQLGSGYAMSAWMERTAILDADAVIAVSQGT
                     KTDILRAYPEIDPGRIHVIYNGIDLNEYQKTSSTSALLEYGVDPSQPYVLFVGRITRQ
                     KGVTHLVDAIPQLPPNTQVVLCAGAPDTPEIAAEMREKVERVRAINPRVIWIEKMVTK
                     PEVIQLYSNCSVFCCPSVYEPFGIINLEAMACRAPVVASATGGILEVVVDGETGYLVP
                     FEQDPVTSFPLHPEKFSRELAGKIAALLADPGKARRFGEAGRKRVEEKFSWTAIADQT
                     IGLYQELIAMRRTASRI"
     gene            complement(2091..3608)
                     /locus_tag="VHE33_19755"
     CDS             complement(2091..3608)
                     /locus_tag="VHE33_19755"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HVT99745.1"
                     /translation="MRFRLYALFLLAAATFCAAQGQASPDVPNAPAPSTFVQSNERTI
                     PSLAIAQIRVPDAPPGAAKRDPPAQETPKTADPQLTMFPHSESARYWISGQSNTIFQG
                     KPGFHSPYEGSNSLDSAGEYKTSQVETLYLGFQPHRSLRYNTDLILDLESAGGRGIGQ
                     ALGLAGFTNLDVVRNPSLGTAPYLGRGEIHQTIGLTSQMVESDRDPFSLATQVPERRF
                     EIRAGKMGANDFLDVNDVASDSHLQFMNWTVDDNGAWDYAADTRGYTVGGVLEYDDRR
                     WSARYGLFAMPTVANGLDLDWAFSRAHGQNWEFELGRFLLRGRKGTTKILGFANTAHM
                     GNYRLAVQHFFSGLTTTPDITSVERFGTVKYGFSWNNEQDITDHLRIGTRFGWNDDHE
                     ESFAYTEAAQTVVIATDYTGGQWHRSSDRLGTAFVSNAIKKDHQNYLKYGGTGFLLGD
                     GGLTYGRENIVESYYTAHAWKGLFIALDLQHIDNPGYNRERGPVWVGAIRGHIDF"
     gene            3866..4141
                     /locus_tag="VHE33_19760"
     CDS             3866..4141
                     /locus_tag="VHE33_19760"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal/formaldehyde-sensitive transcriptional
                     repressor"
                     /protein_id="HVT99746.1"
                     /translation="MPIEQKEQQKLIARVRRIRGQVDAIERSLAQKDHCADVLMLLAN
                     IRGGINSLMAEVLEDHIRLHMLSDRKKSLDSTGLAEDLIDLVRAYLK"
     gene            4226..5707
                     /locus_tag="VHE33_19765"
     CDS             4226..5707
                     /locus_tag="VHE33_19765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HVT99747.1"
                     /translation="MRTETVILWGIAAAVTLAHLLTNQRYGFHRDELQFLTDARHMDW
                     GFVAYPPLTPFLERIQMNLFGLSMDGLRLFSVIGQAVTIVVTGLMARELGGGRLAQVT
                     AVVCMAFAPVPLFNGTEFQYTSFDFLWWVLIAYCVIRLLRSENPRWWLAIGLVTGLGL
                     MTKYAIVFFLAGLLGGFLLTPARRYFASWYFLAGSAIALAIFLPNLIWLVRHDFISYH
                     FLQSIHARDVGEGRAEGFLSGQFMICTNPAAAPLWIGGLVAFFLNRRYRAMAWMYLIP
                     LALFFFGKGRHYYMADGYPALLAMGAVAGERWVASLNRAWRWSVEGIFFTAIAFCGLY
                     IAAIILPFASSGPLMRFALKNNGDLREEIGWQEELATIQGILDALPADQREHTGIFTG
                     NYGEAGAVELLGPAYHLPPPISTTNSAWLRGYPIPPPTTLIVLGHSQKGADRAFTNCK
                     VAGRVRNSLGVDNEESSDHPDIFLCGPPRLPWPEFWKTHQRFG"
     gene            complement(5725..8385)
                     /locus_tag="VHE33_19770"
     CDS             complement(5725..8385)
                     /locus_tag="VHE33_19770"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HVT99748.1"
                     /translation="MNTFGWLTGPGRLMTTEPQTLARLRSRYQDDSAQLRQSFERNGD
                     GSAVIRRRSQIVGTMLRQLWEQAAPSASQNDIALVATGGFGRRELFPCSDVDVLFLCA
                     NEKVEPAYKDQIRACTQAMWDIGLRASPVTRTLKECEKVHPDNLEFTVSLLDRRYLTG
                     GRELYAKFEQEILPSVILREWDTVVQNLAEMARARHAKYGNTIFHVEPNVKECPGGLR
                     DYHLAQWLTLLNSIQANKAWPRSAADDFYGTHDEPESAYDFLSAVRCFLHYRSGRDDN
                     TLDWHAQDEAAARSIGLETRGTSDPAYWMRTWYRQARTVYRRASLLIDAVPPPRRSFY
                     KQFRRKRTPVAGTDFFLEDGRLDLLEGTTVNEAESVLRIFGLMAKHGYRLSYAAEDRI
                     SDALPILAVQMPEGPFLWNCLREVLLGPWAAHALRTMHALGILELIIPEFHGIESLVI
                     RDTWHRYTVDEHTFHVIDNVHRLRQPQHDWEKRLASLLHEIERLDLFFLALLMHDTGK
                     ARRIGNHAANSVELSESLFSRLELDAEERETVRRVIHNHLDMSAAMRRDIFDAETVRA
                     FAEKVATQPQLKMLTLMTYADIRAVSPEALTPWKAESLWQLYMSASNFLDRSVDEIRY
                     HASADPAFFSRIVALIPAVTPGRSTQAVPTEELRSFLEGLPQRYLSSRTPEQIAAHFR
                     MAMQLQEDPVQLTYRSLRQLNEVMLVTRDRPGLFADMAGILSAWGMNIVKADAFSNTA
                     GIIVDTFQFTDTYRTLELNRSEIERFLASIRDALLHRVSVEELLRSRKLKPSRPKVSV
                     ETRLEFDNQSSSHSTLLQIVTQDTPGLLREIVLSFASCRCNIEVALIDTEGEAAIDVF
                     YLTANGGKLEEPEQASLSAALMYRLAQLRQ"
     gene            complement(8454..8792)
                     /locus_tag="VHE33_19775"
     CDS             complement(8454..8792)
                     /locus_tag="VHE33_19775"
                     /inference="COORDINATES: protein motif:HMM:NF012753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HVT99749.1"
                     /translation="MQKIEAVIQPSKLDAVKDALLEAGIEGMTILEARGHGRQKGHTE
                     FYRGREYTVDLLPKIKIEMVVRDEMKDKAVDAILAAARTGKIGDGKIFVSKIDDAIRI
                     RNDERGPSAL"
     gene            complement(8836..10182)
                     /locus_tag="VHE33_19780"
     CDS             complement(8836..10182)
                     /locus_tag="VHE33_19780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HVT99750.1"
                     /translation="MHPRRLIGSLLLLPFLGTSAALAQTPAAPAVSNADNAWMLTSSA
                     LVLMMTGPGLALFYGGLVRRKNVLATMMQSFAMMCIVTVLWALIGYSLAFGPGNSFIG
                     GFHFAFLHNVGLAPDTDYAATIPAQTYMIYQLMFAIITPALICGAFAERMKFSAMCLF
                     LTLWALVVYSPMAHMVWGKGGFLNAALGGAIPCLDFAGGTVVHITSGVSALICALYLG
                     KRVGYPKEPSPPHSVVLSFIGACLLWVGWFGFNAGSAVNASGLATSAFVNTHFATAAA
                     AIGWSVAEWIRNGKPSALGAISGAVAGLVAITPASGFVQPMSALIIGLIAGVFCYLMV
                     TVVKSWFGYDDSLDAFGVHGAGGTIGAILTGIFASSAINPILGAGKATGAIEGNWHQV
                     VNQLAGVAIAWTLSIAGTLVLLKLVDMTVGLRVTREEEIEGLDVTQHGEEGYDFGS"
     gene            10498..11070
                     /locus_tag="VHE33_19785"
     CDS             10498..11070
                     /locus_tag="VHE33_19785"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HVT99751.1"
                     /translation="MSEALRDRRLFGTLESSAKNQEKVLEASYGFEQPEGVFLTTADA
                     AINWVRKNSIWPMTFGLACCAIEMMSMGASRFDVARFGAEVFRPSPRQSDLMVIAGRV
                     SWKMAPVIRRLYDQMPEPKWVISMGACATSGGVFNNYALVQGVNQVIPVDVYVPGCPP
                     RPEQLLYAITLLQEKIQHEQGSFRRALNLQ"
     gene            11400..13100
                     /locus_tag="VHE33_19790"
     CDS             11400..13100
                     /locus_tag="VHE33_19790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99752.1"
                     /translation="MDFRPLNGGRRTLLAVGALVLGAATVVGQTSPPSAPVPHDRQES
                     PSRWDVFMGYSYVGPHGTVSTPLPAGGTTDLNYSSIDYGAIGSVAYYFNRYVGGQIEY
                     ANHPNGANDGAQTAQAGMIFRYPAEGMTWFAHGLAGGVRLGGPNISGDGNYPKHAYTW
                     GPALTVGGGLDYDLPFFDHRFAIRVFQADYEYWHANFGPGPTNPPISGRANVDAARLS
                     AGIVLHFGSIVPPPPVQYACVVNPSSVFPGEQVTVTGTATNLNPKKTPAYTWSGQGLT
                     VGGTNPTSTIDTGNLQPGNYTVTGHVSQGPKAGQSADCTAQFTVKQFEPPTVSCTANP
                     TTVKPGESSTITCNGVSPQSRPLTYSYSASAGSINGTGNTATLSTSGAPAGTITVTGT
                     VQDDKGQTASATTTVNVEAPPPPPAPKTQTLCSIQFDKDKRRPARVDNEAKACLDDVA
                     LNAQQKADASLVVVGNSAPVPAHKHHTRNEMTAEKLAAQRAVNTKAYLVTEKGIDASR
                     IQVRTGSNGQNEVENYLVPAGATFDNDVPGATAVDEGAVKAQPRTAPHRHHRAAKKAQ
                     "
     gene            13335..15380
                     /locus_tag="VHE33_19795"
     CDS             13335..15380
                     /locus_tag="VHE33_19795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99753.1"
                     /translation="MRIRFFLAVSAALLPCLCAAAGFAANPGPWSPTGPDGGDARRFA
                     FSPHDPSRIYLGTTDSWIYVSSDGGSSWSRLARLGTEDDLVVDSLIADRTDPRTLYAG
                     VWAIDHPDGGVYISHDEGRTWNPAPEMRGQSVRALTQATSNPRILVAGTLSGVYRSMD
                     DGRHWREISPPGGKEIHEVESIAIDPYDPEIIYAGTWHLPWKTSDGGAHWKSIDQGII
                     DDSDVFSMIVDPSRPSVMFLSACSGIYRTDNFGYEFRKVQGIPSTARRTRSLLMDPTD
                     RNTVYAGTTEGLYKTTDGGDNWSRMTDPNVIVNDVYVDPRNPKHVLLATDRSGVLASE
                     DGAAGFQASNTGFSQRQVSSLLVDGKSPGTIYAGVINDKVYGGVFVSTDQGRTWKQQS
                     EGLNGHDVFVLAQGQDGALLAGSSNGIFRWDGNSWVHADSVASQPDPPAPAKRERTRR
                     GVGKQGHPIATRVRQDPVELRGRVSALEADGSLWFAAAAQGIFQSADGGASWTPVVLP
                     ESMGVRNGFGGYRTIAAQGQNIFIGRREGMIASEDRGVTWRALALPSGLTALSSLALS
                     PDGTLWAGGREGIFSSSDRGRSWSLQKRVPVVAVNSLTWDASLNRLMITCDVGTVIYA
                     VDPHDESWTWWNAGWTVHAVASLGRRLAAASSYSGVVMQPEVEASVKTGDAVQDARR"
     gene            15384..15842
                     /locus_tag="VHE33_19800"
     CDS             15384..15842
                     /locus_tag="VHE33_19800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HVT99754.1"
                     /translation="MAFSLRAVSPDDLPRLYALDQACFEPGIAWSKAELLYFLKYPGN
                     IGVLVEDESGRLAGFAIAGKQRRQGALFGRLITIDVDASWRRRGVGQLLLQETERQLR
                     TAGATAILLEVSVENLTARSFYERHGFVRTGRIPGYYLGRIDALVMEKPL"
     gene            15897..16721
                     /locus_tag="VHE33_19805"
     CDS             15897..16721
                     /locus_tag="VHE33_19805"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HVT99755.1"
                     /translation="MLRTRVKIACRIAALVSLSCAAAFPQAVRDLPKPTDYVSDIAHV
                     LSAQTIDRINHLCGEVDHEAKAQIAVVTINTTNGQPIQPYAVELEDAWKVGPKGSDRG
                     LLILFAVNDRKYWIETGYGLEGILPDGKVGQIGRQMVPMLRNNDFDGAVTLAVDETSQ
                     IIAQDAGVKLQPLSRWGAQQQQPHATAGQIIGGIVFLILIVIFLIWAGGNGILGFLLG
                     MFLGGGRWGGGGRWGGGGWGGGGFGGGGFGGGGGGGSGFGGFGGGSTGGGGAGGSW"
     gene            16755..17336
                     /locus_tag="VHE33_19810"
     CDS             16755..17336
                     /locus_tag="VHE33_19810"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HVT99756.1"
                     /translation="MMRRGLWIAVAVVGVIVLAGLLVVGSYVSAQRQMVNKQEAVNAQ
                     WSNVQAQLQRRADLIPNLVATVKGYAAHEETVFADVDKARESLLAARDPQGKIAANNQ
                     LDGALGRLLAIAENYPNLKANENFLQLQDQLEGTENRIAVERQRYNRAVQDYNTFIEQ
                     IPNNIWARIGGFQRNNAYFEASPSSQNAPQVHF"
     gene            complement(17349..18713)
                     /locus_tag="VHE33_19815"
     CDS             complement(17349..18713)
                     /locus_tag="VHE33_19815"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HVT99757.1"
                     /translation="MTSLEEIHASSRDIFDRALQACDIPKAFDRHLHFEGKTLVRHPS
                     PRLPSISQSLEGFKEIYVIAFGKAALSMLNALLERLPPKMHVHGVCSAPDVPKKRHRH
                     IRYFEGGHPLPNEDSFAAARATLALLKHAKKTTFIFFLISGGGSAMHELPRDKKITLD
                     DTIAFHETLVASGATITEINTVRKYFSAVKGGRLALAAPAAEKLSLLLADVPLKDLAA
                     VASSPTLPDYSTMAECREILERFHLLEKFPPAVREYFEGLIKEESASGRAKDEQGSAA
                     AFQHAQFDTLLSNHDFVNAARDHAQSLGYKVIIDNTCDDWDYAEAAKYLLARFHELRG
                     EYTRVCLLSSGEVTVNLGKNPGCGGRNQQFALQCALDLARFEGEPLAVLSAGSDGIDG
                     NSPAAGAIADTTTITRARRYNFDPEKTLAQFDACTLFTALGDTVVTGPTGNNLRDLRI
                     LIAG"
     gene            complement(18710..>19324)
                     /locus_tag="VHE33_19820"
     CDS             complement(18710..>19324)
                     /locus_tag="VHE33_19820"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HVT99758.1"
                     /translation="RLEALLNAGKPDAPYAALVCHPHPLGGGTMHNKVVYHAMKAFQS
                     FGLPVLRFNFRGTELSEGEHDYGQGEQEDVRAALDWLDREFHRPVLFAGFSFGSYVGL
                     RACCGDPRVAGLVTLGMPRQAEGRDYSYEFLHECPQPKLFVSGTRDPYGPVADVEAAV
                     AMAQEPKELVWISEADHFFAGKLEEVRKAIRTWACHRFPALQES"
BASE COUNT         3667 a         5859 c         6067 g         3731 t
ORIGIN      
        1 cactattcgc tacgcccgga tggttcgctg cgcgatattt cgccgacgat ccttggactg
       61 ctggggctgc ctcagccgag gcagatgacc ggcggcgatc tgcgggtcag gaagtaaagc
      121 ctgcctccac atcgtggccc gagcccgatt tcggcgagcg tcaggccacg gtgtggtggt
      181 cggcccatgc ccgccttaac ttttctgctg caactccagc cggaacgacc ttgcgcatct
      241 gataacgcgt gagcatccac gctgccgcgc cagccattac acccaacacc cagggacgca
      301 tcatggaaag aaagcctcag tcggaaaatt gcacttccgt gatggatggt cgatctgtcg
      361 ctgcgggatg cacgctgggg aactcaacgt tctggagcgc tcagatcctg gatgcggttc
      421 gtcgcatcgc gatcaattcc tgatacagcc cgatcgtctg gtcggcgatc gcggtccagg
      481 aaaacttttc ttcgacacgc tttcgtcccg cctctccaaa ccgccgcgcc ttgcccggat
      541 cggcgagcag cgctgcaatc tttcccgcca actcgcgaga aaatttctct ggatggagcg
      601 ggaaactcgt gacgggatcc tgctcgaagg gcaccagata gcccgtctca ccgtcgacca
      661 ccacttccag aatgcccccg gttgcgctcg cgacgaccgg cgcgcggcag gccatcgctt
      721 cgaggttgat gatgccgaag ggctcgtaca ccgacgggca gcaaaagacg ctgcagttgc
      781 tgtagagctg gatcacctcc ggcttggtga ccatcttttc gatccagatc acgcgcggat
      841 tgatggcgcg cacgcgctcg actttctcgc gcatctccgc ggcgatctcg ggcgtatccg
      901 gcgcgccggc gcacagcacc acctgggtgt tcggcggcag ctgcgggatc gcgtcgacca
      961 gatgcgtgac gcctttctgg cgcgtgatgc gtccgacgaa caggacatag ggctgggagg
     1021 gatcgacgcc gtactcgagc agcgccgagg tggacgacgt cttctgatac tcgttcagat
     1081 cgatgccgtt gtagatcacg tggatgcggc ccggatcgat ctcggggtac gcgcgcagaa
     1141 tgtcggtctt cgtgccctgg gagacggcga tgacggcatc ggcatcgaga atcgcggtgc
     1201 gttccatcca ggcgctcatc gcgtacccgc tgccgagctg ctccgatttc caggcgcgca
     1261 ggggttcgag actgtgcgtg gtcagcacaa agggaatttt gtaaagcttt ttagccagga
     1321 agccggccat cgccacatac caggtgtggg tgtgcacgat gtcgatcgag ccgatcgagg
     1381 tcatctgcgc caggttcagg ctgaaggttt ccagcgcggt tttgaatttc tcttcggtgc
     1441 cctggctgat ccgggaccac ggctgctcgc cgcgcacttg cagatttccc tggtcggaat
     1501 tttgatctcc ccagcagtgg acttcgactt cgattttctt cgcaagttcg cggctcaggt
     1561 attcaacgtg aacgccggcg ccgccgtaca cgttcggtgg gtactcccga gtcatcagac
     1621 caacgcgcaa gccggcctcc tcaagacgaa ccttcatctt agcgcggcgc aggaaggccg
     1681 tccctgcgga caacaggggc atcaactcac tgctgtgaga ggtttgtcgc ccggtcaggc
     1741 cgcttgttcc agcttgatcc acggcggcag gaacgaaagc atccagcagc cgtcgaattt
     1801 tctggttggc acggtatggc ctgcaaattc cagagtgtat ggcccttccg gcaggtcgcc
     1861 gggctcatcg acaattctgc accgtgtgaa tacacggcct gaactggagc gttccttcca
     1921 cgtggtcagt acgcaattcg cttcgcagtc cagtcccttc agcactccat gtacgcgaac
     1981 aactccctgc aaaatccagc cctcggaata aatttgcgtg cagttctcgg acttgagatg
     2041 aaagggggct cagaagggtt gactgactgg gtatcaaatt ttgttccggt tcagaagtcg
     2101 atgtggccgc ggatcgctcc cacccagact ggtcctcgct cgcgattgta gcccgggttg
     2161 tcgatgtgct ggaggtcaag cgcgatgaaa aggcccttcc acgcatgcgc tgtgtagtag
     2221 ctttccacga tattctcgcg tccgtatgta aggccgccgt cgccgagcag aaatcccgtc
     2281 ccgccgtatt tcaggtaatt ctggtggtct ttcttgatgg cgttgctgac gaaggctgta
     2341 ccgagtctgt ccgacgaacg atgccactgg ccgccggtgt agtcagtcgc gatcaccacg
     2401 gtctgggcgg cttcggtgta ggcgaaggac tcctcatgat cgtcgttcca gccgaagcgc
     2461 gtaccgatcc gcaagtgatc ggtgatgtcc tgttcgttat tccagctgaa gccgtacttc
     2521 acggtgccga agcgttcgac ggacgtgatg tcgggcgtgg tagtcaggcc ggagaaaaaa
     2581 tgctggaccg cgagacggta gttgcccatg tgagccgtgt tcgcaaagcc cagaattttt
     2641 gtcgtgcctt ttcggccgcg aaggaggaag cgaccgagct cgaactccca gttttgaccg
     2701 tgtgcgcggg agaaggccca gtcgaggtcg aggccgttgg cgacggtcgg catggcaaag
     2761 aggccgtagc gggcgctcca tcggcgatcg tcgtattcga gcactccgcc gaccgtgtag
     2821 ccgcgcgtat cggccgcgta gtcccaggct ccattgtcat cgacggtcca gttcatgaac
     2881 tggagatggc tgtcgctggc tacatcgttg acgtcgagaa aatcattcgc tcccatcttg
     2941 ccggcgcgga tctcaaagcg gcgctccggg acctgcgttg ccagcgagaa tggatcgcga
     3001 tcggattcca ccatctggct ggtcaggcca atagtctgat ggatttcgcc tcgcccgagg
     3061 tagggtgccg tgcccaggct cggattgcgg accacgtcga gattcgtgaa tccggcaagc
     3121 cccagcgcct ggccgatgcc gcgtccgccg gcgctttcca gatcgagaat gaggtcggtg
     3181 ttgtagcgca gactgcgatg cggctgaaaa cccaggtaca gcgtttccac ctgcgatgtc
     3241 ttgtattcgc cggcactgtc gagactgttg gagccttcat acggcgaatg gaagccaggc
     3301 tttccctgaa aaatcgtgtt ggactggcct gagatccagt agcgggcgct ctcgctgtgg
     3361 ggaaacatgg tcaactgtgg atcagccgtc ttcggggtct cctgcgccgg cggatcccgt
     3421 ttcgctgccc caggcggggc atcggggacg cggatttgcg cgatggcgag gctgggtatc
     3481 gttcgttcgt tgctttggac gaaggtcgag ggcgcgggag cgttcgggac atcgggagaa
     3541 gcctgtccct gtgcagcgca gaaggtcgct gcggccagca agaacaatgc ataaaggcgg
     3601 aaacgcacgg gatctttctc ctgggaattt gcacaccggt gcgggatgaa ccaactgaca
     3661 cgggtacggg atcgaggaca gatgtcagct cagcgctatt aaccatagtt aattaaaaaa
     3721 gattaaacga ggataacaaa ttcctgtgaa ggaacgggcc ggaaaaggtt ccacccgatc
     3781 gacagttctc ttctttctca gtgcatcaga tatcatatcc ccactggata tcaaacccgg
     3841 tcaggaatac caggggaggc cagcggtgcc catcgagcag aaggagcagc aaaaactcat
     3901 cgcgcgagtt cgccggattc gcggccaggt ggacgctatc gagcgttcgc tcgcgcaaaa
     3961 ggatcactgt gccgacgtgc tgatgctgct ggcaaacatc cgcggcggca tcaacagcct
     4021 gatggccgag gttctggaag atcacatccg gctgcacatg ctgtccgacc ggaagaagag
     4081 cctcgactcg accggactgg cagaagatct catcgatctg gtgcgcgctt atctaaaatg
     4141 aaccgattcc tgcagttctc cgtaacagca cgagaaggga gcgagcatgg cgacagtggc
     4201 ggcgacgcaa tcagctgaaa aaagcgtgcg gaccgagact gtgatcctgt gggggattgc
     4261 cgcggcggtc actcttgcgc atttgctcac caaccagcgc tacggcttcc atcgcgatga
     4321 gctgcagttc ctcactgacg cccggcatat ggactggggg ttcgtcgcct atccgccgct
     4381 gacgccgttt ctggaacgca tccagatgaa cctctttgga ctgtcgatgg acggtctgcg
     4441 gctcttctcc gtgatcgggc aggcggtcac gatcgtcgtc accgggctga tggcgcgcga
     4501 actggggggt gggcgtctgg cccaggttac ggcagtggtt tgcatggcgt tcgcaccggt
     4561 gccgttgttc aacggaacgg aatttcagta cacaagcttc gatttcctct ggtgggtgct
     4621 catcgcctac tgcgtcatcc ggctgctccg ctcagagaat ccgcgatggt ggctggcgat
     4681 tgggctcgtg accggcctgg gtctgatgac caaatacgcc atcgtgtttt ttctggcagg
     4741 gctgctggga ggattcctgc tgacgccggc acgccgctat tttgccagct ggtatttcct
     4801 cgccggttcg gcgatcgctc tggctatctt cctgccgaac ctgatctggc tggtgcgcca
     4861 tgatttcatt tcctaccact ttctgcagtc gatccacgcg cgcgatgttg gcgaaggccg
     4921 ggccgaggga ttcctgagcg ggcagttcat gatctgcacg aatccggctg cggcgccgct
     4981 gtggattggc ggcctggtgg cgttttttct gaaccggcgt tatcgcgcga tggcgtggat
     5041 gtacctgatc ccgctggcgc tgtttttctt cggcaaagga cggcattact acatggccga
     5101 cggctatccg gctctgctgg ccatgggcgc agtggcgggg gagcgctggg tagcatcgtt
     5161 gaaccgggca tggcggtggt cggtggaagg cattttcttc acagccattg cgttttgcgg
     5221 cctgtacatt gcggcgatca ttctgccgtt cgcatcgagc ggaccgctga tgcgcttcgc
     5281 tctcaagaac aacggcgacc tgcgcgaaga gatcggctgg caggaagagc tggccacgat
     5341 acagggcatt ctcgatgccc tgcctgcgga ccagcgcgag catacgggca tctttaccgg
     5401 aaactacggt gaggcgggtg cggtggagct gctcggaccg gcgtatcatc tgcctccgcc
     5461 gatcagcacg accaactcag cctggctgcg cgggtatccg attccgcctc ccaccacgct
     5521 gattgtgctg ggccattcgc aaaagggcgc ggaccgggca tttaccaact gcaaagtggc
     5581 agggcgggtg cggaactcgc tgggcgtgga caacgaagag agcagcgatc atccagacat
     5641 ttttctgtgc gggccgccgc gactcccgtg gccggaattc tggaagaccc atcagcgctt
     5701 tgggtgacgc ggaccatgcc tccgctattg gcgcagttgc gccaggcggt acatcagcgc
     5761 ggcgctgagc gaggcctgct cgggttcctc gagcttgccg ccgttggcgg tcagataaaa
     5821 cacgtcgatg gcggcctcgc cttccgtgtc gatgagcgcc acttcgatat tgcagcggca
     5881 actggcgaag ctgagcacga tctcgcgtaa caatccgggc gtatcctgag tgacgatctg
     5941 cagcagagtg ctgtgcgacg aggactgatt gtcgaactcc agtcgcgtct cgacactgac
     6001 cttgggccgc gacggcttca gcttgcgtga gcgaagcagc tcctccaccg agacccggtg
     6061 cagcagagcg tcgcgaatgc tggcgagaaa gcgctcaatc tcagatcggt tcagctccag
     6121 ggtgcgatag gtatcggtga actggaaggt gtcgacgatg atgcccgcgg tgttggagaa
     6181 ggcgtcggcc ttgacgatgt tcatacccca cgccgagaga atgccggcca tgtcggcaaa
     6241 caagccgggt ctgtcccgcg tgaccagcat cacttcattc agctggcgca gagaccggta
     6301 cgtcagctgc accgggtctt cctgcaactg catggccatg cggaagtggg cggcaatctg
     6361 ctccggcgtc cggctgctga ggtagcgctg cggcaggcct tcgaggaagc tgcgcagctc
     6421 ttcagtagga accgcctgcg tagaacggcc gggtgtgacg gcggggatga gagcgacaat
     6481 gcggctgaag aacgccggat cggcagacgc gtggtagcgg atttcgtcca cgctgcgatc
     6541 gagaaagttc gacgcgctca tatagagctg ccacaggctc tctgccttcc atggcgtcag
     6601 cgcctcgggg gaaaccgccc ggatatcggc ataggtcatc agggtgagca tcttgagctg
     6661 gggctgagtc gccacttttt ccgcaaaggc gcgaaccgtt tcggcgtcga agatgtcgcg
     6721 gcgcatcgcc gccgacatgt cgagatgatt gtggatcacg cggcgtacgg tctcgcgctc
     6781 ctccgcatcc agctcgaggc gcgagaagag gctctcgctc agctcaacgc tgttggcggc
     6841 gtgattcccg attcggcgcg cctttcccgt gtcgtgcatc agcagcgcca gaaagaacaa
     6901 atcgaggcgc tcgatttcat ggagcaggct ggcgagccgc ttttcccagt catgctgcgg
     6961 ctgccgcagc cgatgcacat tgtcgatgac gtggaaggta tgctcgtcga ccgtgtagcg
     7021 gtgccaggta tcgcgaatca ccagcgactc gatgccatga aactcgggga tgatcagctc
     7081 gaggatgccg agagcatgca tggtgcgcag ggcatgcgcg gcccacggcc cgagcagcac
     7141 ctcccgcaga cagttccaca gaaatggtcc ttcgggcatc tgcaccgcca gaatgggcag
     7201 ggcgtctgag atgcggtctt cggcggcgta gctgaggcgg tagccatgct tggccatcag
     7261 gccgaaaatc ctcagcacgc tctcggcttc gttcacggtc gtgccttcga gcagatcgag
     7321 ccggccatcc tcgaggaaaa aatcggtccc ggccaccgga gtccgctttc ggcgaaactg
     7381 tttgtagaag gagcggcgcg ggggcggaac ggcatcgatg agcagagaag cgcggcgata
     7441 gacggtgcgc gcctggcgat accaggtgcg catccagtac gccgggtccg aggtgcctcg
     7501 cgtctcaagc ccaatggagc gcgccgcggc ctcgtcctgt gcgtgccagt ccagggtatt
     7561 gtcgtcgcgc ccggagcgat agtgcaggaa gcatcggacg gccgagagga agtcgtaggc
     7621 cgattccggc tcgtcgtggg ttccgtaaaa atcgtcggcg gccgagcgcg gccatgcctt
     7681 gttcgcctgg atggaattca gcagagtgag ccactgcgcc aggtgatagt cgcgcaggcc
     7741 gccgggacac tccttcacgt tgggctcgac gtggaagatg gtattgccgt atttggcgtg
     7801 ccgcgcccgc gccatctccg ccagattctg caccaccgtg tcccattcgc gcagaatgac
     7861 cgagggcaga atctcctgtt cgaactttgc gtacagttca cgcccgccgg tcaggtagcg
     7921 gcgatccagc agggaaaccg tgaattccag attgtccgga tgaacctttt cgcattcttt
     7981 cagcgttcgg gtgaccgggc tggcgcgcaa tccgatgtcc cacatcgcct gggtgcaggc
     8041 acggatctga tctttgtagg cgggctcgac tttttcgttg gcgcacagaa aaagcacatc
     8101 gacgtcggaa caggggaaga gttcgcggcg gccgaatccg ccggtggcga ccagcgcgat
     8161 gtcgttctgc gatgcagagg gcgcggcctg ttcccacaac tgacgcagca tggttccgac
     8221 gatctgcgaa cggcgccgga taaccgccga gccgtcgccg ttgcgctcaa agctctgacg
     8281 caactgggca gagtcgtcct gatagcgact gcggagacgc gccagagttt gcggctcagt
     8341 ggtcatcaat cggcctgggc ccgtcagcca tccaaacgtg ttcatcgttc cggcaggaac
     8401 gatgggtgcg gctgaacttc gctcggacca gcggaacgga cgcgggaccg aaatcagagc
     8461 gcggaggggc cgcgctcatc gttgcgaatg cgaatcgcgt catcgatctt cgaaacgaag
     8521 atcttgccgt cgccgatttt tcccgtgcgg gcggctgcga ggatggcgtc gacagctttg
     8581 tctttcatct cgtcgcggac caccatctcg attttgattt tgggcaggag atcgaccgta
     8641 tattcgcgac cccggtagaa ctcggtgtgg cccttctgcc ggccgtggcc gcgggcttcg
     8701 agaatggtca tgccttcgat gcccgcttcg agcagggcgt ccttgactgc gtccagtttc
     8761 gatggctgaa tcactgcttc gatcttttgc attgcgcgct caaatcctga ctgaatgttg
     8821 agtatacgtc cagaatcacg aaccgaaatc gtagccttct tcgccgtgct gtgtcacgtc
     8881 cagaccctcg atctcctcct cgcgggtcac acgcaggcca acggtcatat ccacgagctt
     8941 cagcaggacc agcgtgcccg cgatggacag cgtccacgcg atggccacgc cggccagctg
     9001 attcaccacc tggtgccagt tgccttcgat cgccccggtc gccttgcctg cgccgaggat
     9061 ggggttgatc gcgctggaag cgaagatgcc ggtgagaatg gcgccgatcg tgcctcccgc
     9121 accgtgaacg ccaaaggcgt ccagggagtc atcgtagccg aaccagctct taaccaccgt
     9181 gaccatcaga tagcagaaca ctcccgcgat gaggccgatg atgagggccg acatcggctg
     9241 tacgaagccc gatgcgggcg tgatggccac cagcccggcc accgccccgg aaatagcgcc
     9301 aagggcactg ggcttgccgt tgcggatcca ctcggcgacg ctccacccga tggccgcggc
     9361 cgccgtggca aagtgcgtat tcacgaaggc gctggtcgcc aggccgctgg cattcaccgc
     9421 gctgccggcg ttaaatccaa accaccccac ccacaacagg caggcgccga tgaagctgag
     9481 cacgacggag tgcggcggcg atggctcttt ggggtacccg acgcgcttcc cgagatacag
     9541 ggcgcagatc agcgccgaaa cccccgacgt gatgtggacc accgtgccgc ccgcgaagtc
     9601 gaggcagggg atcgctcccc ccagggcggc gttcagaaat ccgcctttac cccaaaccat
     9661 atgcgccatg ggcgaataca ccaccagcgc ccacagtgtc agaaagaggc acatcgcgct
     9721 gaacttcatg cgctcggcga aagctccgca gatgagtgcc ggggtaatga tggcaaacat
     9781 cagctgatag atcatgtacg tctgcgccgg gatggttgcg gcgtagtcgg tgtcgggcgc
     9841 gaggcccacg ttgtgcagga acgcgaagtg aaagccgccg atgaaactgt tccccgggcc
     9901 gaaggccagg ctgtagccga tcagcgccca cagcacggtg acgatgcaca tcatggcgaa
     9961 gctctgcatc atcgtggcga ggacattctt gcgccgaacc aggccgccgt agaacagcgc
    10021 caggccgggg ccggtcatca tcagtaccag cgccgagctg gtcagcatcc aggcgttgtc
    10081 ggcgttggag acggcgggtg ctgcgggcgt ttgtgcgagg gcggcggaag tgccaagaaa
    10141 aggaagaagc agaagggacc caatcagacg acgagggtgc atatcactcc gttttgcgta
    10201 gacggaaata tgtcacgacg tggtgaccgg caggcgatcc tgcaacggat gatcgacggg
    10261 gagatccgtg ggtttgcagg attgacctga gtctcactgt tacatatttt tcgcgaatcg
    10321 aaaacagaga aatcgccgct aagccgtcgc cccatcccgc ccggaagatt tcgccgttga
    10381 ttgcgtccat cccggttcca gccatcgatg aattcattcc ggaatacggg acgaaggtac
    10441 cctggacaga gtcttcacca ggcgatccgg accgcgccgg gaaggtatca tgggtgaatg
    10501 agcgaagcat tgcgcgaccg caggcttttc ggcacgctgg aaagcagcgc gaagaaccag
    10561 gaaaaggttc tggaagcgag ctatggtttt gagcaaccgg aaggcgtttt tctgaccacc
    10621 gccgacgcgg ccatcaactg ggtccggaaa aactccatct ggccgatgac tttcggcctg
    10681 gcctgctgcg cgatcgagat gatgtcgatg ggcgcttcgc ggtttgacgt agcgcgcttc
    10741 ggcgccgaag ttttccggcc ctcgccgcgg cagtccgatc tgatggtgat cgccggccgc
    10801 gtttcctgga aaatggcgcc cgtcatccgc cggctctacg accagatgcc ggagcccaaa
    10861 tgggtaattt cgatgggagc gtgcgcgacg tcgggcggcg tatttaacaa ttatgcactt
    10921 gtgcagggag ttaatcaggt gatccccgtc gacgtctacg tacccggctg tccaccccgt
    10981 cccgagcaac tcctgtacgc catcacgctt cttcaggaga agatccagca cgagcagggt
    11041 agtttccgcc gcgcgctgaa cttgcaatga tcaaggcaag ttgagtactc taatgtcaac
    11101 gcagcgctgg tcgtttcatg gcgctgcagc atctccccct gattcagaac gttagttagt
    11161 tgtcgaatag tagtcccatg cagaggttaa cgacttcgac acgtagtggg ttttggttaa
    11221 ccacgccggg gacttattga aataatcaat gctggcggtc gatacgcttg tggtgacgac
    11281 aggttttcag atgtctccgt cgggcccccg tcatccgtca gcacgaatcc tgggcagcaa
    11341 attcaggttt atagctgctt tgttatgagc catcattcgt attttcggag gatagacgca
    11401 tggattttcg tccattgaac ggtggacgcc gaacgttact cgccgtgggc gccctggtgc
    11461 ttggcgcggc aacggtagtg ggacaaactt cgcccccgtc ggctcccgtc ccccacgacc
    11521 ggcaggaatc cccttcccgc tgggacgtat ttatggggta ttcatacgtc ggacctcacg
    11581 gcacggtctc cacgcccctg cccgccggag gaacgaccga tctgaattac tcttcgatcg
    11641 actacggcgc gatcgggagt gttgcttact atttcaaccg ctatgtcggc ggccagatcg
    11701 agtacgccaa ccaccccaac ggcgcgaatg acggtgccca gaccgcccag gccggcatga
    11761 tcttccgtta tcccgccgag ggaatgacgt ggttcgccca tggactggcg ggaggtgtcc
    11821 gcctcggcgg cccaaacatc tccggcgacg ggaattaccc caagcatgcc tacacatggg
    11881 gtccggcgct cactgtcgga ggcggcctgg actatgacct gccgttcttc gaccaccgtt
    11941 ttgctatccg ggtctttcag gctgactacg aatactggca cgcgaatttc ggcccgggtc
    12001 cgacaaatcc cccgatcagc ggccgcgcga atgtcgatgc ggcccggctc agcgccggaa
    12061 tcgtgctcca cttcggcagc atcgttcccc cgcctccggt ccagtatgcc tgcgtcgtga
    12121 acccctcctc tgttttcccg ggtgaacagg tcaccgttac cggcaccgcc accaacctca
    12181 atccgaaaaa aacgccggcc tatacctgga gcgggcaggg cctgaccgta ggcgggacca
    12241 acccgacgtc caccatcgac accggcaacc tgcagccggg caactacacc gtgacgggcc
    12301 atgtcagcca gggcccgaag gccggccagt ccgctgattg caccgcgcag ttcacggtga
    12361 agcagttcga gcctccgacg gttagctgca cggccaatcc aaccaccgtg aagcccggcg
    12421 agagctccac catcacctgc aacggcgtga gcccgcaaag ccgtccgctg acctacagct
    12481 actcggcttc cgctgggtcc atcaacggga ccggcaacac ggcgaccctc tccacctccg
    12541 gcgctcccgc gggcaccatt acggtgaccg gcacagtgca ggacgacaag ggccagacgg
    12601 cttcggcgac aaccacggtc aacgtggaag caccgccgcc gccgcccgct ccgaaaacgc
    12661 agacgctctg ctcgatccag ttcgataagg acaagcggcg tcccgcgcgt gtggacaacg
    12721 aagccaaggc ttgcctggac gacgtagccc tcaacgctca gcagaaggcg gatgcctctc
    12781 tggtggtggt ggggaactcg gctccggttc cggcgcacaa acaccacacc cgcaacgaaa
    12841 tgactgccga aaaactggcg gcacagcgtg cggtcaatac caaggcctac ctggtgacgg
    12901 agaagggcat cgatgccagc cgcatccagg tgaggacggg cagcaacggt cagaacgagg
    12961 tggagaacta cctggttccg gcaggcgcaa ccttcgacaa cgacgtgccg ggagcaacgg
    13021 cagtcgacga aggcgcagtg aaggcccagc ctcgcaccgc gccacaccgt catcaccggg
    13081 cggcgaaaaa ggcccagtag tccaacccgc gaaacaaagc agggctggcc ggaattccgg
    13141 tcagccctct ttcttttggc aggcgctgtc cgttccggtt gggaatagcc ccttccctgt
    13201 tgcccggaat gggaccttgg ctcctcacgc gccgtcgaat tgccggcaaa acccgttagc
    13261 atggaatcgg gcggtgcgcc ttgcgaagcc cggctgccct ggcctgcccg ctttaattac
    13321 gaatgatgaa gtggatgcga attcgatttt tcctcgccgt ctctgcggct cttctgcctt
    13381 gtttgtgcgc tgctgccggt ttcgcggcca atcctggccc ctggtccccg acggggcccg
    13441 atggcgggga tgcccgccgt ttcgccttca gtccgcacga ccccagccgc atttatctgg
    13501 gcacaaccga ttcctggatt tacgtctctt ccgacggcgg ttcttcgtgg tcgagactcg
    13561 cccggctcgg taccgaagac gatctggtag tcgatagcct cattgccgat cgcaccgatc
    13621 ccaggacact ctatgccggg gtttgggcca tcgaccatcc cgacggcggc gtttacatca
    13681 gccacgatga aggccggacg tggaaccctg cccccgagat gcgcggccag tccgtgcgcg
    13741 ccctcaccca ggcgacatcc aatccgcgca tcctggttgc cggtacgctg agcggggtct
    13801 atcgaagcat ggacgatggc cggcactggc gcgagatcag cccgccaggc ggcaaagaga
    13861 ttcacgaagt cgagtcgatc gcgatcgatc cctatgatcc ggagatcatt tacgcgggca
    13921 cctggcatct tccctggaaa accagcgacg ggggcgcgca ctggaagagc atcgatcagg
    13981 gaattattga cgattccgac gttttttcga tgatcgtcga cccttcccgc cccagcgtga
    14041 tgtttctcag cgcctgctcc ggtatctacc gcaccgacaa cttcggctat gagtttcgca
    14101 aggttcaggg gatcccctcg acggcgcggc gcacgcgttc gctgctgatg gatcccaccg
    14161 accgcaacac ggtgtatgcc ggcaccaccg aggggcttta caagaccacc gacggcggcg
    14221 acaactggtc gcgcatgacc gatcccaacg tgatcgtgaa cgatgtctac gtcgatccgc
    14281 ggaacccgaa gcacgtcctt ctggccaccg atcgcagtgg cgttctggcc agtgaggatg
    14341 gggctgccgg ttttcaggcc tccaatacag gcttttcgca gcgccaggtt tcgtcgctgc
    14401 tcgtggatgg caaatccccg ggaaccatct acgccggggt catcaacgac aaggtgtacg
    14461 gaggcgtctt cgtcagtacc gaccagggca ggacctggaa gcagcagagc gagggtctga
    14521 acggccacga cgtttttgtg ctcgcgcaag gccaggatgg cgccctgctg gcgggaagca
    14581 gcaacggcat ctttcgctgg gatgggaatt cctgggtgca cgccgactcg gtcgcgagcc
    14641 agcctgatcc cccggcgccg gcaaagcggg aaagaaccag gagaggggta gggaaacagg
    14701 gtcaccccat tgcgacgaga gtccgacagg atcccgtcga gctgcggggg cgcgtgagcg
    14761 cgctcgaggc agacggatcc ctgtggtttg ccgcggcggc gcagggaatt tttcaaagtg
    14821 ccgatggcgg agcttcgtgg acccccgtcg tgctgccgga atcgatgggt gtgcgcaacg
    14881 gcttcggcgg ctatcggacc atcgccgccc aggggcagaa catcttcatc gggcgtcgtg
    14941 aaggcatgat tgcttccgaa gacagaggcg tcacctggcg cgcgctggct ctcccttccg
    15001 gactcacggc cctgtccagt ctcgcgctct cgcccgacgg cacgctgtgg gccggaggcc
    15061 gggaaggcat tttctcctcc agcgatcggg gacgctcgtg gagccttcag aagagggtcc
    15121 cggtcgtcgc cgtcaacagc ctcacctggg atgcgtcgct gaaccgcctg atgatcacct
    15181 gcgatgtggg cacggtcatt tacgccgtcg atccccacga cgaatcctgg acgtggtgga
    15241 acgccgggtg gacggtgcat gcggtggcct cgctgggccg gcggctcgcg gcggcatcgt
    15301 cctacagcgg cgtggtgatg cagccggagg tcgaggcctc ggtaaaaaca ggcgatgctg
    15361 tgcaggatgc gcgaagataa gcggtggcct tttcgcttcg tgctgtgtcg ccggacgacc
    15421 ttcccaggct ctacgccctg gatcaggcct gcttcgagcc cggcatcgcg tggtcgaagg
    15481 cggagctgct gtactttctg aaatatccag gcaatatcgg cgttctggtc gaggacgaat
    15541 ccggccgcct cgccggtttt gcgattgccg gcaaacagcg gcgtcagggc gcactgttcg
    15601 gccggctcat caccatcgac gtggatgcct cttggcggcg tcgcggcgtc gggcagctgc
    15661 ttctgcagga aaccgagcgt cagctgcgaa cggcgggcgc cacagcgatc cttctcgaag
    15721 tctcagtcga aaatctcacg gcccggagtt tttacgagcg tcatggtttt gtgcgcaccg
    15781 ggcgcattcc cggttactac ctgggccgta tcgacgccct ggtgatggaa aagccgcttt
    15841 gagagggtga atgcgcggcg cttcctcttc gcgcacacgc gactagaatg aagacaatgc
    15901 tacggaccag ggtgaagatc gcgtgcagga ttgcggcgct cgtgtccctc tcctgtgccg
    15961 ccgcctttcc gcaggccgtc agggaccttc ccaaacccac cgactacgtt agcgacatcg
    16021 cgcacgtgct gtcggcccag accatcgacc ggatcaatca tctctgtggc gaggtggatc
    16081 acgaggcgaa ggcacagatc gccgtggtca ccatcaacac gacgaatggt cagcccatcc
    16141 agccgtatgc cgtcgaactg gaagacgcct ggaaggtcgg cccgaagggt tccgatcgcg
    16201 gcctgctgat tctgttcgcc gtgaacgacc gcaagtactg gatcgagacc ggctatgggc
    16261 tggaaggaat tcttccggac ggcaaagtcg ggcaaatcgg ccgccagatg gtgccgatgc
    16321 tgcgcaacaa cgactttgac ggcgcggtca ctctggcggt cgacgagacc tcccagatca
    16381 tcgcccagga tgcaggtgtg aaactgcagc cgctgtcgcg atggggcgcg caacagcagc
    16441 agccgcacgc gaccgccggc cagattatcg gcggcatcgt cttcctcatc ctgatcgtga
    16501 tcttcctcat ctgggctggc ggcaacggca ttctgggatt cctgctcggc atgttcctcg
    16561 gcggcggacg ctggggcggt ggaggccgct ggggcggagg cggttggggc ggtggcggtt
    16621 tcggcggcgg aggcttcggc ggtgggggtg gaggcggcag cggttttggc ggcttcggcg
    16681 gcggcagtac cggcggcggc ggcgctggag gcagttggta acatcgaatc ggatgggtaa
    16741 gggagagcaa cgcaatgatg cgtcgtggtc tttggatcgc agtagcggtt gtgggagtga
    16801 ttgtcctggc cggcctgctg gtggtcggca gctacgtcag cgcgcagagg caaatggtga
    16861 acaagcagga agccgtgaac gcgcagtggt cgaacgtgca ggcgcagctg cagcgtcgcg
    16921 ccgatctgat tccgaatctc gtggccacgg tgaagggcta cgcagcgcac gaggaaaccg
    16981 tgttcgccga tgtcgataag gcccgcgagt ctctgctggc cgctcgtgac ccgcagggaa
    17041 aaatcgccgc gaacaatcag ctggatgggg cgctggggcg gctgctggcc atcgccgaga
    17101 actacccgaa tctcaaggcg aacgagaact tcctgcagct ccaggatcag ctggagggca
    17161 cggagaatcg tattgcggtg gaacggcagc gctacaaccg cgctgtgcag gactacaaca
    17221 ccttcatcga gcagattccg aataacatct gggcgcgcat cggcggtttc cagcgcaaca
    17281 acgcttactt cgaggcgagt ccttcatcgc agaatgcgcc gcaggtgcat ttctgaggcc
    17341 acggccagct accccgcgat caggatgcgc agatcgcgca gattattgcc ggtcggtccg
    17401 gtgacgacgg tatcgcccag cgcggtaaac agcgtgcagg catcgaactg cgccagggtt
    17461 ttctcgggat cgaaattgta acggcgcgcg cgcgtaatgg tcgtggtgtc ggcgatcgcg
    17521 cccgccgccg ggctgttgcc gtcaatcccg tccgatcccg cactcagaac cgccaacggc
    17581 tcaccttcaa accgggccag atcgagagcg cattgcagcg caaactgctg gttgcgcccg
    17641 ccgcatccag gatttttgcc cagattcacc gtgacctcgc cgctcgaaag caggcagacg
    17701 cgcgtgtatt cgccgcgcaa ctcgtggaag cgcgccagca gatatttcgc cgcctcggcg
    17761 taatcccagt cgtcgcaggt gttgtcgatg atgaccttgt agccgagcga ctgcgcgtga
    17821 tcgcgcgcgg cgttcacgaa gtcgtggttc gacagcagcg tgtcgaactg cgcgtgctga
    17881 aaggccgccg cggatccctg ctcatccttc gcccgccccg atgccgactc ctctttgatg
    17941 agtccctcga agtactcgcg cacagccggc ggaaacttct ccagcagatg gaagcgctcg
    18001 agaatctccc tgcactccgc catggtcgaa tagtccggca gcgtcggcga agacgcaacc
    18061 gcggcaagat ccttgagcgg aacatcggcc agtagcagcg agagtttctc cgcggcggga
    18121 gccgcaagcg ccagacgccc ccccttcacc gcggaaaaat acttgcggac ggtgttgatc
    18181 tccgtgatgg tcgcgccgct ggcaaccagc gtctcgtgga acgcgatcgt gtcatccagt
    18241 gtgatcttct tgtcgcgcgg cagttcgtgc atcgccgatc cgccgccgct gatcagaaaa
    18301 aagataaacg tggtcttctt cgcatgcttc agcagcgcca gcgtggcgcg cgctgcggcg
    18361 aacgaatcct cgttcggcag cgggtgcccg ccctcaaaat agcgaatgtg ccggtggcgc
    18421 tttttgggta catccggtgc cgagcacacg ccgtgcacat gcatcttcgg cggcagccgt
    18481 tccagcagcg cattcagcat actcagcgcc gccttgccaa acgcaatcac gtagatctct
    18541 ttgaatccct caagcgattg ggagatggaa ggcaaacgcg gggagggatg ccgtaccagg
    18601 gttttgcctt cgaagtgcag atgccggtcg aaggcttttg ggatatcgca ggcctgcagc
    18661 gctcgatcga agatgtctct ggaacttgcg tggatttctt cgagagaggt catgattcct
    18721 ggagagccgg gaagcggtgg cacgcccacg tgcgaatggc ttttctcact tcctccagct
    18781 tacccgcaaa aaaatgatcc gcttcggaaa tccacaccag ctctttcggt tcctgcgcca
    18841 tcgcgaccgc ggcctcgacg tcggcgaccg ggccgtacgg atcgcgcgtg ccgctcacga
    18901 acagcttggg ctgcgggcac tcgtgcagga attcgtagct gtagtcgcgc ccttccgcct
    18961 gcctcggcat gcccagcgtg accaggccgg ccacgcgggg atcgccacag caggcacgca
    19021 agccgacata cgagccaaac gaaaacccgg caaacagcac cgggcgatga aattcgcgat
    19081 ccagccagtc gagtgcggca cgcacatcct cctgttcgcc ctggccgtaa tcgtgctcgc
    19141 cttcgctcag ctccgtcccg cgaaagttga atcgcagcac cggcagcccg aaactctgaa
    19201 acgctttcat cgcgtgataa acgaccttgt tgtgcatcgt tccgccgccc agtggatgcg
    19261 gatggcagac cagagccgcg tacggcgcat cgggcttgcc ggcattcaga agcgcttcca
    19321 gccg
//
LOCUS       DASZYN010000780         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_54611, whole genome shotgun sequence.
ACCESSION   DASZYN010000780 DASZYN010000000
VERSION     DASZYN010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54611"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VHE33_19825"
     CDS             complement(<1..839)
                     /locus_tag="VHE33_19825"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HVT99759.1"
                     /translation="MKTETFWEATGPFRRVGAIVLGVLLVCTGFAFAQTGNGAITGTI
                     ADAQGAMVGGASVKITQVNTGTTRTVTTNGAGQFNVPSLPPATYSVTVEAQGFKSYQQ
                     NIVLLADQIRDMDVKLEVGAITQRVTVETSAVQVNTISPTLGQVIEQSRLVDIPLNGR
                     NAADLTLLVPGAVNLSANNAGTQQGDTKQVPGAEAISVNGTRPDQIGYNLDGASNEDL
                     MSNTNNPFPFPDALQEFSVQTNSFDAQYGNNAGAVVNVVTKSGTNNWHGSAFEFVRNR
                     VFNA"
     gene            1245..>1535
                     /locus_tag="VHE33_19830"
     CDS             1245..>1535
                     /locus_tag="VHE33_19830"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HVT99760.1"
                     /translation="MTSALESIRVVELTEALAGPYCAMLLGDLGADVIKVERPKIGDQ
                     ARRWGPPYCGSESAYFLAANRNKRSIAIDYDDGAGREVLERLLARADVFLVNQ"
BASE COUNT          259 a          402 c          527 g          347 t
ORIGIN      
        1 gcgttgaaga cccggttgcg gacaaactcg aaggcgctgc catgccagtt gttggtgcct
       61 gatttcgtga ccacgttgac gacggcgccg gcgttgttac cgtactgggc gtcgaagctg
      121 ttggtctgca cgctgaattc ctgcagcgca tcagggaaag ggaagggatt gttggtgttg
      181 ctcatcaggt cttcgttgct ggcgccgtcg aggttgtagc cgatctggtc gggacgggtg
      241 ccattgacgg agatcgcttc cgcgcccgga acctgctttg tatcgccctg ctgggtgccc
      301 gcattgttcg ctgacagatt cactgcgccg ggaacgagga gtgtgaggtc ggcggcgttt
      361 ctgccgttga ggggaatgtc caccaggcgc gactgctcga tgacctgacc gagagtgggg
      421 ctgatggtat tgacctggac cgcggaggtt tcaacggtga cgcgctgggt gatcgcaccg
      481 acttccagct tcacgtccat gtcgcggatc tgatcggcga gcagcacaat gttttgttgg
      541 tagctcttga agccctgcgc ttcgacggtg actgagtagg tggctggagg cagggacgga
      601 acgttgaact ggccggcgcc gttggtggta acggttcggg tggttccggt gttgacctgc
      661 gtgatcttga cgcttgctcc gcccaccatg gcgccctgag cgtcggcgat ggttccggtg
      721 attgcgccgt tgccggtctg ggcgaaggca aaaccggtgc ataccagcag gacgcccaga
      781 acgatggctc cgacacgccg gaatgggccg gtggcctccc agaaagtctc cgttttcatt
      841 gaatcctcct aaaatcgaca gcttcactgc aattttgttt gagacataga ctatgagtct
      901 cttgatggca cgcgaattta cgctgccgtc gtgaggactg tcaatgaaat tgtggggctt
      961 acggaggcgg tatatccatg ctggtaaaca ggaaaccctg cgatggttgg attgttgggc
     1021 gattacagag gggcgggagg ttgttacgat cgtgccgcgc gcacgcgggg cgtccgcaat
     1081 ttgttgttga gcaaaacaga tgaggcggcg gagcagctgg ggacggcgga agttcagggg
     1141 cagttcgcct gcctcggcgg cccgtgcctg gggtctgttt tctatcgccg ctttatactt
     1201 ctccgtcgaa cctcgctcgg cagttgcgag gggcgaccct ctccatgact tctgctctgg
     1261 aatccatccg ggtggttgaa ctcaccgagg cgctcgctgg cccctactgc gcgatgctgc
     1321 tgggcgatct tggcgcggac gtcattaagg tggagcgccc gaagatcggc gaccaggcac
     1381 gccgctgggg cccgccgtac tgcgggagcg aatccgcgta ttttctggcg gccaatcgca
     1441 ataagcggtc gattgccatc gattacgacg atggtgcggg ccgcgaggtg ctggagcggc
     1501 tgctggcgag ggcggatgtg ttccttgtga atcag
//
LOCUS       DASZYN010000781         1543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_877180, whole genome shotgun sequence.
ACCESSION   DASZYN010000781 DASZYN010000000
VERSION     DASZYN010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877180"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VHE33_19835"
     CDS             <1..745
                     /locus_tag="VHE33_19835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99761.1"
                     /translation="DLDQIIARIRHHGRMAGMITNGYLLVPERIQRLNKAGLDHLQIS
                     IDNVNPDEISKKSLKVLDKKLEMLSEHADFHVNINSVVGGGIKNPNDALVIGRRALSL
                     GFTSTIGIIHDGDGQLKPLGDEERKVWSEMRSWKKKNYSRFNHFQVNIANGQPNDWRC
                     RAGSRFLYICENGLVHYCSQQRGYPGVPLAGYTKADLRREFLTEKGCAPNCTVSCVHQ
                     ISFIDYWRSPQRPNPPAAANADGLVNIQL"
     gene            complement(758..1102)
                     /locus_tag="VHE33_19840"
     CDS             complement(758..1102)
                     /locus_tag="VHE33_19840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99762.1"
                     /translation="MTCCADVAEIVTKTFRELGFPLLLSQQQAKLLETCQIAREVIAG
                     ERNSWAAASHLEYSIWKWETTIPEVADIFAIHDEIDWEPIYRRSVTDLYVALIEAFAR
                     AALIPDSEIAKY"
BASE COUNT          328 a          549 c          371 g          295 t
ORIGIN      
        1 cgacctcgat cagatcatcg cccgcatccg ccaccacggc cgcatggccg gcatgatcac
       61 caacggctac ctgctcgtcc ccgagcgcat ccagcgcctc aacaaggccg gcctcgacca
      121 cctccagatc tccatcgaca acgtcaaccc cgacgagatc tccaagaaat cccttaaggt
      181 cctcgacaag aaactcgaga tgctctccga acacgccgac ttccacgtca acatcaattc
      241 cgtcgtcggc ggcggcatca agaatcccaa cgacgccctc gtgatcggcc gccgcgccct
      301 ttccctcggt ttcacctcca ccatcggcat catccacgat ggagacggcc agctgaagcc
      361 cctcggcgac gaagaacgca aagtctggtc cgagatgcgc tcctggaaga aaaagaatta
      421 ctcccgcttc aatcacttcc aggtcaacat cgccaacggc cagcccaacg actggcgctg
      481 ccgcgccggc tctcgcttcc tctacatctg cgagaacggc ctcgtccact actgctccca
      541 acagcgcggc taccccggcg ttcccctcgc cggctacacc aaagccgacc tcaggcgcga
      601 attcctcacc gaaaaaggct gcgctcccaa ctgcaccgtc agctgcgtcc accagatctc
      661 gttcatcgac tactggcgat ccccgcagcg cccgaatccc ccagcggcag caaacgcaga
      721 tggtctggtg aacattcagc tgtaaggagc ctcagactca atacttcgcg atttcgctgt
      781 cggggatcaa agccgcccgc gcgaacgcct cgatgagtgc cacgtacaga tcggtcaccg
      841 atcgtctgta gatcggttcc caatcgatct cgtcgtggat tgcaaaaatg tctgcgacct
      901 cagggattgt cgtctcccat ttccagatcg aatattccag atgactggca gcagcccagg
      961 agttcctttc tcccgcgatg acctctctcg cgatttggca cgtctccagc aacttcgcct
     1021 gctgttgcga tagcaagaga ggaaatccca gctcgcggaa cgtctttgtg acgatttctg
     1081 cgacatcagc acagcacgtc acgttcgttt cgccggcgag ccgtctcaga gctggactgt
     1141 catatccagc ttccaggagg tctgctgcga ttcctggcat tttctcgccg gagattccgt
     1201 gacttgccca ctgaagagcg attcgcatcg gcgatagcaa cggctccggc ctggggactt
     1261 ccggaaactc ctcagcgaac gaaatcgctg gactcgagta gatacccgac aaatcgttcc
     1321 ctgacaaacc cgagaagaat gcttgcagcg agaccctggc caccgatcaa ttatgcagcg
     1381 ggaaccagca attcccgccc atctcaggct ggtgttcccg accattcgtg cttctcagcg
     1441 ctcgcctcca cagccccgcc ggactcgtgc cgcaccttct tccgcgacgc gcgcttccgc
     1501 gtgtacatat cggagtccgc cgcgacgaac agctcttcgc agc
//
LOCUS       DASZYN010000782         2757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_428433, whole genome shotgun sequence.
ACCESSION   DASZYN010000782 DASZYN010000000
VERSION     DASZYN010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428433"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..487)
                     /locus_tag="VHE33_19845"
     CDS             complement(224..487)
                     /locus_tag="VHE33_19845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99763.1"
                     /translation="MNHSIALTFIGMLMTAAVGLITGWAIGDLTRRYRHGVEQLDALR
                     RQFSQPNSAALFQQRQLREIRGILRDLHKMLRAVSKGLEQRPS"
     gene            complement(555..1151)
                     /locus_tag="VHE33_19850"
     CDS             complement(555..1151)
                     /locus_tag="VHE33_19850"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HVT99764.1"
                     /translation="MKRAALYMRVSTVDQHPETQTLDLRQLAAQRGFPIVAEYTDTIS
                     GVKARRPGLDQLMRDARHGRFDVLVIWAFDRLARSTRHMLEVLDELNHLGIEFVSFRE
                     QIDTGGPLGRAIMTIVGAVAELERSLIVERVRAGMRRARLEGRHIGRRPLDLDRTAIL
                     RDRQHGQSLGQLAQSYRVSRTTIHRLLHEHAPSAELSL"
     gene            complement(1148..1330)
                     /locus_tag="VHE33_19855"
     CDS             complement(1148..1330)
                     /locus_tag="VHE33_19855"
                     /inference="COORDINATES: protein motif:HMM:NF020835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partition protein ParG"
                     /protein_id="HVT99765.1"
                     /translation="MSSIPRTETTETKRLNVNIPIQLHNQFKSLAAARGENMTDLLLK
                     FVSNYVREHSPRGRRR"
     gene            complement(1415..1702)
                     /locus_tag="VHE33_19860"
     CDS             complement(1415..1702)
                     /locus_tag="VHE33_19860"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HVT99766.1"
                     /translation="MSMHRQYPLPYSTDTFCPGRHPNDIWRWYFADAIRNLRLARGLS
                     IQKAARLAGMEFTQWEAMESGHPPVDSECLRPVAEVLRVDWEELSRLRRLP"
     gene            1840..2097
                     /locus_tag="VHE33_19865"
     CDS             1840..2097
                     /locus_tag="VHE33_19865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99767.1"
                     /translation="MATPWYSDQILPFDITLAASNEYGALAGARLLGVEILTEGTGVS
                     IDDTVTETQATFVARGIEPLQAVQTNFTAAALAAAQVALSA"
BASE COUNT          484 a          863 c          887 g          523 t
ORIGIN      
        1 gcatatgcca ttacgatatc cgctaacgaa agatgctgac gccgacggtg tatctttcga
       61 gggagtgcag gtgcgggctc ggtaacgtaa cagttgcctt catacttgcg ctgattctgt
      121 ggtctgagcc gcgaacccgc cgctctgagg ggaagattgt tccctggtcc acaccttgtg
      181 gaacggccgg atcttatcga tccggccatt gattttgtgg ggtttaggag ggtcgttgtt
      241 cgaggccttt tgaaacggcc ctgagcatct tgtgaaggtc acggaggatg ccgcggattt
      301 cccggagctg gcgctgctgg aagagggcgg cggagttggg ctgggagaac tgacggcgca
      361 gagcgtcgag ttgttcgacg ccgtggcggt aacgacgcgt gaggtcgccg atggcccagc
      421 cggtgatcag cccaacggcg gcggtcatca gcataccgat gaaggtgaga gcgatggagt
      481 ggttcatgca ggcctcctca tggccggttc gagacggagc aggacggtgg ggcggtagat
      541 gacagcgagc accttcatag cgacagctcg gcggatgggg cgtgctcgtg aagcaggcgg
      601 tggatggtgg tccttgagac gcggtagctc tgcgccagct ggccgaggct ctggccgtgc
      661 tgacgatcgc gaaggatcgc ggtgcggtcg agatccagcg gtctgcgtcc gatatgccgg
      721 ccttcaagcc gggcgcggcg catgcccgcg cgcacccgtt cgacgatgag gctgcgctcc
      781 aactcggcaa cggctccaac gatggtcatg atggcgcgac ccagcggtcc gccggtgtcg
      841 atttgttcgc ggaaggagac gaactcgatg ccgaggtggt tgagctcgtc gagaacctcg
      901 agcatgtggc gggtggagcg agcgaggcgg tcgaaggccc agattacgag cacatcgaag
      961 cggccatggc gcgcatcgcg catcagctgg tcgagtcccg gccggcgcgc cttcacgccg
     1021 gagatcgtat cggtgtactc cgccacgatg gggaagccgc gctgagcggc gagctggcgg
     1081 aggtctaagg tctgggtctc gggatgctga tcgacggttg agaccctcat ataaagagcg
     1141 gcgcgtttca ccggcgccgc cctcggggag agtgttcgcg gacatagttg ctgacgaact
     1201 tcagcagcag gtcggtcatg ttctcgcctc gggccgcagc cagagatttg aactggttgt
     1261 gcaattgaat cggtatgttc acgttcaagc gcttcgtctc cgttgtttcg gtgcgtggga
     1321 tggaactcac ctccttcctc agaaggtgag cttatacggt tatgagtaac gcggccagag
     1381 cgggggaggg aggctccggc cccgggttga gggttcaggg cagacggcgc agccgggaca
     1441 gttcctccca gtcgacccgc aggacctcgg ccaccgggcg caggcactcg gaatcgacgg
     1501 gcggatggcc cgactccatg gcttcccact gggtgaattc catgccggcc aggcgggcgg
     1561 ccttctggat gctgagtccg cgggcaaggc gcagattgcg gatcgcgtcg gcgaagtacc
     1621 accgccagat atcgttcggg tggcggccgg ggcaaaaggt gtcggtggaa taaggcagag
     1681 gatactgacg atgcatactc atgtactcat tactcctgag aggcttcccc gtcaagaggg
     1741 tcagcccgat tgacttacag gctgagtgtg accccgaccc gccttccagc caaagccagg
     1801 atatacctgc cgcagctggc cgtcggacag gacacggagt tggccacccc ctggtactcc
     1861 gatcagatcc ttcccttcga catcaccctt gccgcctcga acgaatatgg cgcgctggcg
     1921 ggtgcaaggc tgctcggtgt ggagatcctg accgaaggca ccggcgtttc gatcgacgac
     1981 accgtcaccg aaacccaggc taccttcgtc gcccgcggca tcgagccgct gcaggccgtg
     2041 cagaccaact tcacggctgc ggctctggct gcagcgcagg tcgctctctc ggcgtaacct
     2101 ggtgcctccc cggccggagc acatattttt ccccgcgcgc gcgaaaccct gcctccggcc
     2161 gggcccgcaa cctcgatccg caacgcccga tccacaacgc caagtcccac cctccgctgc
     2221 gcacgttccg cgactccgag ttcgcgcctg cggtccggct cttctcgtct gccgatcgtt
     2281 ctacgaacct gcccaaccgg acaggcggtt cgggctcgag gctcgacagg gctgacctgt
     2341 tcgcgggaga aaaacactgc tctccgggag gaactgtctt cctgctgtgg gggctgccca
     2401 ggctcctctc tctcgccgag actctcctcc cgaggtcccc tggctctatc tatttagaag
     2461 gagaagagac cgcttccggg tcgctcctcc accggtcaaa acccgcccat cctgagcccc
     2521 ccggcgccgc cgcgccggac gctccaaccc tcctggctct ctggttacat ccggcccaat
     2581 caagcctgca cgttttccgg agctgaaatc acagcgcagg ccgggttccc ccgtgtttcc
     2641 aacgtgacaa gcgcaacgtg gaaccaatta accccgcaga atgccctccc ggactccctc
     2701 ttagcctgcc tcttccgtta ctcatcagta ccttttcccc cagccccagg ctgcccc
//
LOCUS       DASZYN010000783         4147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_249165, whole genome shotgun sequence.
ACCESSION   DASZYN010000783 DASZYN010000000
VERSION     DASZYN010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249165"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1034
                     /locus_tag="VHE33_19870"
     CDS             132..1034
                     /locus_tag="VHE33_19870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99768.1"
                     /translation="MQILALVWRRPSLVALELLWRWTFGIPLLALLGFVGTHIWIEQE
                     ASLRATGVFEFSLQYAWQSALQVADAIDVLKPPVLHVLAWLLPLAIVAWAVASGLGRN
                     VVVRRFRTQTAWRPATMIVLQMLRAVALIGTYLVWYLAVRWAANFTLTRATSNPAAQD
                     EPNLVLYCALVIIFSLGIFTLWALLSWVFSIAPLLVLLQNLGIGGSLARSLQLGPLKG
                     KLIEINLVMGIAKLGMIVLAMVFSATPLPFEAEIHGSALYAWWAVVTVLYLVASDFFQ
                     VARVVAFLELWEFPRLPQSGPPLR"
     gene            1141..2070
                     /locus_tag="VHE33_19875"
     CDS             1141..2070
                     /locus_tag="VHE33_19875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT99769.1"
                     /translation="MRLLFAVCVSALIPLAALSQTPATSDPASAAADKAYTTQDWPAA
                     ESQYAALVRQHPESTRFWYRLGFSARANKHYDVALEAMQKAKTLGAAVGYPAQLADYE
                     LAITSAAAGQSSQALQYLKSSADAGFMQTNRLSSDPEWNALRSNEQFIALAKEVQHNA
                     TPCDDPQFRQFDFWLGDWDVASAGDNIHRGSSHVSKEMNGCVVWENWTSAGNPYFGKS
                     YNTWNPNLNRWEQYWVDTAAGVMFFHGALKDNVMDYWTDDIPQTTGGTLQRHLQFFNL
                     GPDKVRQFSQGSTDGGKTWHTEYDFIYTRVAKD"
     gene            2530..2751
                     /locus_tag="VHE33_19880"
     CDS             2530..2751
                     /locus_tag="VHE33_19880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99770.1"
                     /translation="MRVFQVGRGGAVFAMSGITILNAGRAGLLRNLLTRWSAINCGPA
                     TTPASNNSPTGKIHAHRTLFGQTFIGPEV"
     gene            2799..>4147
                     /gene="guaA"
                     /locus_tag="VHE33_19885"
     CDS             2799..>4147
                     /gene="guaA"
                     /locus_tag="VHE33_19885"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF000848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HVT99771.1"
                     /translation="MPTPSIVILDFGSQYTQLIARRIREQNVYSAVLPCTAKLDDLRA
                     LNPVGIVLSGGPCSVYDSDAPPADPAILNLGLPVLGICYGLQFIAHHLGGRVRSAAKR
                     EYGHADVELVDAANPLFAELPASMHVWMSHGDEALDLPPGFHLTAKTANAVAGIANPE
                     RRIWAVQFHPEVHHTPLGTQVLRNFLFNICGARPDWTPEHFIQSTVAAIREKVGKGHA
                     ICALSGGVDSSVAAVLVNRAIGDQLTCVFVNNGVLRKDEFEKVQQNLREKLGLKLVAV
                     DASKRFLLKLRGVTDPEGKRKIIGNEFISVFDDEAARIAEEDSGANTKLEWLVQGTLY
                     PDVIESSSVKGPSQTIKSHHNVGGLPEDMQLKLIEPLRDLFKDEVRRIGRDLGMPEEI
                     LQRQPFPGPGLAVRILGEVTAERVALLQEADDIVVSEIKNAGLYTKIWQSFAVLLPV"
BASE COUNT          787 a         1429 c         1125 g          806 t
ORIGIN      
        1 ggaaaagaag ttgtgcgcat catcgtcgtg ccgcgaaagc tgatcaacat tgtggtgaag
       61 tagaacctgt gcacgtatcg acttcgcagg cagaaccgcc gtacgcgcag caccgtggaa
      121 cgcagtcttt cgtgcagatc ctcgcgctcg tctggcggcg cccctctttg gtagcgctgg
      181 aactgctctg gcgctggacc ttcggcatcc ccctcctcgc gctcctcggt ttcgtcggaa
      241 cgcatatctg gatcgagcag gaagcatctc ttcgcgccac cggcgtcttt gaattttcgc
      301 tccagtacgc atggcagagc gcgctgcaag tcgccgacgc catcgatgtg ctcaagcccc
      361 cggtgctgca tgtcctcgcc tggctcctgc cgctcgcaat cgtcgcatgg gccgtcgcct
      421 cgggactggg acgcaatgta gtcgtgcgcc ggttccgcac acaaacggca tggcgccccg
      481 ccaccatgat cgtcctgcag atgttgcggg ccgtagcgct gatcgggact tacctggtct
      541 ggtatctcgc cgtccgctgg gcggcaaact tcacgctgac tcgggccacg tcgaatcccg
      601 ccgcccagga tgagcccaac ctggtcctct actgtgcgct cgtgatcatc ttctccctcg
      661 gcatcttcac cctttgggcg ctcctgagct gggttttctc catcgcgcct ctcctggtcc
      721 tgctgcagaa cctcggtata ggcggaagcc ttgcacggag cctgcaactc ggtccgctca
      781 aaggaaagct gatcgagatc aacctcgtga tgggtatcgc caagctcgga atgatcgtgc
      841 tcgccatggt cttctcagca acgccgcttc cgtttgaagc cgagatccat ggcagtgctc
      901 tctacgcttg gtgggcagtc gtgacggtcc tttatctggt ggcgtccgac ttcttccagg
      961 tcgcgcgcgt cgtcgcgttt ctggagctgt gggaattccc ccgccttccg caaagcggcc
     1021 cgcctttgcg ctgatccaca ctctcatcac cggcccaccg cagttcgtca caatcacggc
     1081 gccatgcgtc gcatccctgc cggatgatgc ttcgtgcccc tgcaaaccca cggaggtccg
     1141 atgcgactgt tattcgccgt ttgcgtttcc gcccttatcc ccctggccgc tctttcgcag
     1201 acgcctgcaa cttccgatcc cgcctcagcc gcagccgaca aagcctatac cacgcaggat
     1261 tggcccgccg ccgaatcgca gtatgccgcc ctcgtccggc aacatccgga aagcacgcgc
     1321 ttttggtacc gcctcggttt ctccgcgcgc gccaacaagc attacgacgt cgctcttgaa
     1381 gctatgcaaa aagcaaagac cctcggcgcc gcggtcggct atccggcgca gcttgccgac
     1441 tacgagctcg ccatcaccag cgccgccgcc ggccagtcat cgcaggccct gcagtatctg
     1501 aagtcctccg ctgacgccgg attcatgcaa accaatcgtc tctccagcga cccggagtgg
     1561 aacgccctgc gctccaacga acagttcatc gcgctggcga aagaggtgca gcacaacgcc
     1621 accccctgcg acgaccctca gttccgccag ttcgatttct ggcttggcga ttgggacgtc
     1681 gcctctgcgg gcgacaatat ccatcgcggc tccagccacg tctcgaaaga aatgaacggc
     1741 tgcgtcgtct gggagaactg gacctccgcc ggcaatccct acttcggcaa aagctacaac
     1801 acctggaatc ccaatctgaa tcgctgggaa caatactggg tcgacacagc cgccggcgtc
     1861 atgttcttcc acggcgccct gaaggacaac gtcatggatt actggaccga cgacatcccc
     1921 caaactacgg gcggaaccct gcagcgccac ctccagttct tcaatctcgg cccggataag
     1981 gtgcgtcagt tctcccaggg ctcaaccgac ggcggcaaaa cctggcacac cgaatacgac
     2041 ttcatctaca cccgcgtcgc aaaggactag ccgtcaaggc gtacgcgcct tcggctcctc
     2101 tgccattgcc gaaaccagat gatgctggca aatgctgaga aaactcccca aatccccagc
     2161 gcaatcatta gcagcgtgaa ttgagagtct gcccgatcgg ctggagttgc ggtgaggaga
     2221 taatctattg ctgctccaat gccgattacc gccacgggta acatcaacgt gagatcccac
     2281 cagcgcaacc gccctgtgtt tccggatttt ggactcttgc tggcgtcgat acaacggccg
     2341 agcgaccacc aaaacggcag cgcatagatt ggatagagca ttgcagtcca cgcccagcga
     2401 tcaaaaccaa aggttgggcc aattccgatc gtgggcttgc gtctccagag gacaacagcg
     2461 gcgtccaccg caagcccagg gacgttggcg agtgcgacgg cgtgggttcg ttcaggcagg
     2521 agatctgaga tgcgcgtctt ccaggtcgga cgcggcggcg cagtgtttgc gatgtcggga
     2581 attacgatat tgaacgcggg acgagcgggg ctattgagga atttgctcac ccggtggtcg
     2641 gcgatcaatt gcggacctgc aacgactccg gccagcaata attcacccac cggcaagatc
     2701 cacgcccatc gcaccctttt cggccagacc ttcatcggtc ctgaagttta gcaccggccg
     2761 tttctttaca ccgcccccaa tcccgggcaa actggattgt gcccacccct tccatcgtca
     2821 tccttgactt tggctcgcag tacacgcagc tgatcgcgcg ccgcatccgc gagcagaacg
     2881 tgtactcggc cgtcctgccc tgcaccgcaa agctcgacga tcttcgcgcg ctgaacccgg
     2941 tcggcatcgt cctttcaggc ggcccctgct cggtctacga ttccgacgcg ccgcccgccg
     3001 atcctgcaat cctcaacctc ggcctgcccg tgctcggcat ctgctacggg ctgcagttca
     3061 ttgcgcatca cctgggtggt cgcgtgcgat cggctgcgaa gcgcgagtac ggtcacgccg
     3121 acgtggaact ggtcgacgca gccaaccccc tcttcgccga gctgcccgct tccatgcacg
     3181 tctggatgtc ccatggggac gaggctctcg accttccgcc cggcttccat ctcacagcga
     3241 aaaccgccaa cgcggtggcc ggcattgcca atcccgagcg ccgcatctgg gccgtccagt
     3301 ttcatcccga agtgcaccac acgcccctcg gcacgcaggt gctgcggaat ttcctcttca
     3361 acatctgcgg cgcccggccc gactggaccc ccgagcattt catccagtcc accgtcgccg
     3421 ccatccgcga aaaagtcggc aaagggcacg ccatctgcgc cctttccggc ggcgtggatt
     3481 cctcggtcgc cgcagtcctg gtcaaccgcg ccatcggcga tcagctcacc tgcgtcttcg
     3541 tcaacaacgg cgtcctgcgc aaagacgagt tcgagaaggt gcagcagaat ctgcgcgaga
     3601 agctcggcct taagctcgtt gctgtcgatg ccagcaagcg cttcctgctc aagctccgcg
     3661 gtgtcaccga ccccgaaggc aagcgcaaga tcatcggcaa cgagttcatc tccgtcttcg
     3721 acgacgaagc cgcgcggatc gccgaggaag acagcggcgc caacacgaaa ctcgagtggc
     3781 tcgttcaggg caccctctac cctgacgtca tcgaatcatc ctccgtcaaa ggtccctccc
     3841 agaccatcaa gagtcaccac aacgtcggcg gcctccccga ggacatgcaa ctcaagctca
     3901 tcgaaccgct gcgcgacctc tttaaggatg aagtccgccg catcggccgt gacctcggca
     3961 tgccggaaga gattttgcag cgtcaaccct tccccgggcc aggtctcgcc gtccgcatcc
     4021 tgggcgaggt caccgccgag cgcgttgccc tcctccagga agccgacgac atcgtcgtaa
     4081 gcgagatcaa aaatgccggg ctctacacga aaatctggca gtcgttcgct gtcctgctgc
     4141 cggtcaa
//
LOCUS       DASZYN010000784         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1150271, whole genome shotgun sequence.
ACCESSION   DASZYN010000784 DASZYN010000000
VERSION     DASZYN010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150271"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..139
                     /locus_tag="VHE33_19890"
     tRNA            63..139
                     /locus_tag="VHE33_19890"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:97..99,aa:Arg,seq:acg)
     gene            338..1867
                     /gene="istA"
                     /locus_tag="VHE33_19895"
     CDS             338..1867
                     /gene="istA"
                     /locus_tag="VHE33_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HVT99772.1"
                     /translation="MALLSVIRRWHLRDGHSIREIARRTGLSRNTIRKYLRSDEVEPR
                     FKAPDRPSKLDAYAERLAAWLRTEANRPRKQKRTIKQLHADLMSLGYEGSYGRVAAFA
                     RDWKADRQREMQTSGRGTFVPLAFEPGEAFQFDWSEDWAVIGGERTKLQVAHTKLSYS
                     RAFAVRAYPLQTHEMLFDAHNHAFRVLGGVPRRGIYDNMRTAVDKVGRGKDRQVNARF
                     SAMASHYLYEAEFCNPASGWEKGQVEKNVQDARHRLWQPMPRFPSLEALNDWLEQRCR
                     ELWQQIPHGLRPGTIEDIRAEEARFLMPMPRLFDGFVEYTKRVTPTCLVHLERNRYSV
                     PASFANRPVSLRVYPERIVVAAEGQIVCEHRRIIERSHDGPGRTVYDWRHYLAVIQRK
                     PGALRNGAPFTELPDAFKRLQQHLLRKPGGDREMVEILSLVLQHDEQVVLSAVEMALE
                     AGVPTKVHILNLLHRLVDGKPIAAPVVDAPQALSLAKEPQANVERYDALRQVREARHA
                     S"
     gene            1857..>2113
                     /locus_tag="VHE33_19900"
     CDS             1857..>2113
                     /locus_tag="VHE33_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011255145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT99773.1"
                     /translation="MRHDPASGAIVIMLRSLKMHGMAQAVTELTEQASPAFEAAIPIL
                     SQLLKAETAEREVRSVAYQLKSARFPVYRDLAGFDFASSEI"
BASE COUNT          395 a          670 c          672 g          376 t
ORIGIN      
        1 gcgcacggtt cgccgggccc gctggattgc tgtccggtgc ggtccattga aggaccggtt
       61 gcgcgcccgt agctcagctg gatagagcac cagactacga atctgggggt caggagttcg
      121 aatctcttcg ggcgcgccat ctcgcttttt ccactgtaaa tctcacctaa tgccttgaat
      181 ggaaaggcga aattaggtgt tcgaagtccc tcatttcggc tatatgttga tttccgccga
      241 gagctgaccc gggattgagg gatttttcca tcgagaagtg acccatgttt gaacccgccc
      301 ctgctggctg attgcagggg attttggagt gatagacatg gcgttactga gcgttatccg
      361 acgctggcat ctgcgggacg gacattcgat ccgggagatt gcacggcgca cgggactgtc
      421 gcggaacacg atccgcaaat acctgcgatc cgacgaggtg gagccccgct tcaaggcgcc
      481 ggaccggccg agcaagcttg atgcttatgc ggaacggctg gcggcctggc tgaggacgga
      541 ggcgaacagg ccccgcaagc agaagcgcac gatcaagcag ttgcacgccg atctgatgag
      601 cctcggctat gagggctcat atggtcgtgt cgccgccttt gcccgtgact ggaaagcgga
      661 tcgtcaacgc gagatgcaaa cgagcgggcg cggcaccttc gtgccgctgg cgttcgagcc
      721 cggggaggcg ttccagttcg actggtcgga agactgggcg gtcatcggcg gcgagcgcac
      781 gaagctgcag gtggcccata ccaagctcag ctacagccgc gccttcgccg tgcgggccta
      841 tccgctgcaa acgcatgaga tgctgttcga tgcccacaac cacgccttcc gggttctggg
      901 cggcgttccc cggcgcggca tttacgacaa tatgcgcacg gcggtcgaca aggtcggccg
      961 cggcaaggac cgtcaggtca acgcccgctt ctcggccatg gccagccact acctctacga
     1021 agccgagttc tgcaatccgg cctcgggctg ggagaagggg caggtcgaga agaacgtcca
     1081 ggatgcgcgc catcggctat ggcaacccat gccacgcttc ccatcgctgg aagcgctgaa
     1141 cgactggctg gagcagcgct gccgggagct gtggcaacag atcccccatg gtctcaggcc
     1201 gggaacgatc gaggacatcc gggccgagga agcacggttc ctgatgccga tgccgcgcct
     1261 cttcgatggc ttcgtcgaat acaccaagcg ggtcacgccg acctgcctcg tccatctgga
     1321 gcgcaaccgc tacagcgtgc cggcgtcctt cgccaaccgg ccggtcagcc tgagggtcta
     1381 tccggaacgg atcgtggttg ccgcggaggg gcagatcgtg tgcgagcacc gccgcatcat
     1441 cgagcggtcg catgacggcc ccggtcgcac cgtctacgac tggcgtcact atctggcggt
     1501 catccagcgc aagcctggtg ccttgcgcaa cggcgcgccc ttcaccgaac tgccagatgc
     1561 cttcaaacgg ctacagcagc atctgctcag gaagcccggc ggcgaccggg agatggtcga
     1621 gatcctgtcg ctcgtcctgc aacatgacga gcaggtcgtg ctgtcggccg tggagatggc
     1681 actcgaggcc ggcgtcccca ccaaggtcca tatcctcaat ctcctgcacc ggctggtcga
     1741 cggcaaaccg atcgcggcac ccgtcgtgga tgcgccgcag gcgctgagtc tggccaaaga
     1801 gccacaggcc aacgtcgagc gctacgatgc gttgcgccag gttcgggagg cccgccatgc
     1861 gtcatgatcc cgccagcggc gctatcgtca tcatgctccg gagcctcaag atgcacggca
     1921 tggcgcaggc cgtcaccgaa ctcaccgaac aggcttcacc cgccttcgag gccgccatcc
     1981 ccatcctgtc gcaactgttg aaggccgaga cggccgagcg ggaggtcagg tccgtggcct
     2041 atcagctcaa gagtgcacgc ttccccgtct atcgcgacct ggccggcttc gactttgcca
     2101 gtagcgagat caa
//
LOCUS       DASZYN010000785         2800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1150666, whole genome shotgun sequence.
ACCESSION   DASZYN010000785 DASZYN010000000
VERSION     DASZYN010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150666"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1442
                     /locus_tag="VHE33_19905"
     CDS             <1..1442
                     /locus_tag="VHE33_19905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVT99774.1"
                     /translation="AKEDWQFAAEGLKKAAPVPEVYGQSDNLERISFGVIASIEMFKA
                     TGDPLYSREGIDLGNQILASQERQLQPWTIPMTGYFYTGPDRKYLFHRFHMGEEEQPI
                     VALAQLCNALPDDPNWMKWYSAIVLHTKYYQEAGARLDAPYNVLPAGIYRISGAAVLP
                     ETPDWRPLETASRAMFVAEVKEGFHLGGDAYLRRYPVWFQFRGNSSVLLSEAKALSVA
                     AQLRGDIDDENLAQQQAQWLIGRNPFSASIMYGEGYDWTPLYSVRSGQMVGAIPVGIE
                     TKGNADIPYWPNQICWTYKEVWTQPAGEWIWLMRDLNGPAVLDGEVDPGSKGAVTILN
                     EQSHATYTASIAPNGAFRATLPQGHYRITYGEARTSLTALVGGTYSVDLRRDHAVAFS
                     ALSEPGSAPGSIVLHATVHGAGQHTFSLRTDNLDISGAEIVSVDLGKQGSQVVSWRAR
                     VVDRSTPWVAVVLQDGLLRGHAELSGLPQ"
     gene            complement(1474..2199)
                     /locus_tag="VHE33_19910"
     CDS             complement(1474..2199)
                     /locus_tag="VHE33_19910"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HVT99775.1"
                     /translation="MSTMPRRSEEPAYKRIQGAIRRRIDNGQLKPGNAVPSERELARI
                     HQVSLMTARHALTELARDGVVERRHGAGTFVAPPRVQFNKLLGYTEQMASLGLIAHSR
                     VVASTVVSHESEIAARLGLGSAARIVRLERVRLAQSEPVAIEVGYWSEAEFPDLLRIT
                     LNGTSLFSALENDCGVALSYADEEIDATDADQRMADLLRVGRNTPLLRIRQLIFSTTG
                     KAVVYVTGFYRSGRHTLRIRRLR"
BASE COUNT          579 a          861 c          790 g          570 t
ORIGIN      
        1 ctgcaaaaga agactggcag tttgccgctg agggtttgaa gaaagccgca cccgtgcccg
       61 aggtttacgg acaaagcgat aacctggaga gaatttcttt cggagttatc gcttcgatcg
      121 aaatgttcaa ggccaccggc gaccccctct actcccgaga gggcatcgat ttgggcaacc
      181 agatccttgc ctcacaggaa cgccagctgc agccgtggac catccccatg accggctact
      241 tttataccgg tccggatcga aaatatcttt ttcaccgctt tcacatgggg gaggaagagc
      301 agcccatcgt tgcgctggcg caactctgca acgctctgcc cgatgatccg aactggatga
      361 agtggtattc ggccatcgtc ctgcatacga agtattacca ggaagcaggg gcgcgtctgg
      421 acgctcctta taacgtcctt cctgccggaa tctaccgcat cagcggtgcc gccgttcttc
      481 ccgaaactcc cgactggaga ccgctggaaa ccgccagccg tgcgatgttc gtagcagagg
      541 taaaagaggg tttccacctg gggggcgatg cttacctccg ccgctatccc gtctggttcc
      601 agtttcgcgg taactccagc gtgctgcttt ctgaagcgaa agcgctctcc gtggcggcac
      661 agctgcgcgg agacatcgac gatgaaaatc tcgcgcagca acaggcccag tggttaatcg
      721 gcagaaatcc gttctccgcc agcattatgt atggcgaagg ctatgactgg acccccctct
      781 acagcgtccg gtccggccag atggtgggtg caattccggt aggcattgag acgaaaggca
      841 atgcagatat tccgtactgg cccaatcaga tctgctggac ctacaaggag gtctggactc
      901 agcctgcggg cgagtggatc tggctcatgc gcgatctcaa cgggcccgcg gttctcgatg
      961 gtgaggtcga tcccggcagc aagggcgcgg tcacaatcct gaacgaacag tctcacgcaa
     1021 cctacacggc gtccatcgcg ccgaatggcg ccttccgcgc aactcttccc cagggacact
     1081 atcggataac atacggcgaa gcccgcactt cactcacagc actggtcggc ggcacttact
     1141 cggtcgacct gcgacgcgac catgctgttg ccttctctgc cttatccgaa cccggctcag
     1201 ctccaggatc gatcgtcctc cacgcaactg tccacggggc cggccaacat accttttctc
     1261 ttcgcacaga caatctcgac atctccggcg cggagatcgt gtctgtggat ctgggaaagc
     1321 aagggagcca ggtcgtgagc tggcgcgccc gcgtcgttga tcgatccact ccgtgggttg
     1381 cggtcgtact ccaggacggc ttactccgtg gacacgcaga actgagcggt ctcccacagt
     1441 agagggactc attgaggccg cctggacagt agttcatcgc aaacgccgga tgcgcagcgt
     1501 gtggcgcccg gaacgataga accccgtaac atacaccacc gcttttcccg tggttgagaa
     1561 aatcagctgg cgaattcgca gcagcggcgt gttccggcca acacgcagta aatcggccat
     1621 ccgctgatcg gcatccgtcg catcgatctc ctcatcggca taagacaatg caacgccgca
     1681 atcgttctcc agcgccgaaa acagcgatgt gccgttgaga gtaatccgga gcaaatcggg
     1741 gaattcagcc tccgaccagt aacccacctc gatcgcaacg ggttcgcttt gggcaagtcg
     1801 cacgcgttca aggcgaacga tgcgagcagc cgaaccaagg cccagccgcg ctgctatctc
     1861 agactcatgg ctaaccaccg ttgaggcgac gacccgcgaa tgtgctatga gtccaaggct
     1921 cgccatctgc tccgtgtagc caagcagttt gttgaactgt acccgtggcg gcgcgacaaa
     1981 cgtgcctgcg ccgtggcggc gttcgaccac gccgtcccga gctaattctg tcagagcgtg
     2041 gcgcgccgtc atcagactca cctgatgaat gcgcgccagt tcccgttccg aaggcactgc
     2101 attcccaggt ttcagctggc cgttatcgat ccggcggcgg atcgctccct gaatcctctt
     2161 gtaagccggt tcttcggatc ggcgcggcat ggtgctcatg gtcagatctc cacaaggccg
     2221 gggcagattg ctgtgtcggt cgagatcgtg ttgctctgtt caggaaacag atcggcttgc
     2281 tgcgccacgc taagggaggg cgcgcggctg aatcctagca gagtgctata gagctagcaa
     2341 gtcaatgctg cgaagcccgg gaacttcccc gtggtgcacc acagaagacc tgtgctcgtg
     2401 cccccgtggt cgggaatcgc ctctccgcgc gctcggcagc tcttgtctga agcaaagtcc
     2461 cgacatttcc acaacctcag tctctcgtcg cttgacacga tggccctgat tgctctatag
     2521 cattaggcac gtgctataga ggactccgcg ggcgttcttc tggcgcagag agacaaagtt
     2581 cccagtcccg gcagacccgc tgcagtcccg ccgttgccat tgcttcacat tgtgtttctt
     2641 ctcgatgatc agatttcatc gacccaggaa gtgggctcct cagtgcagtc cccggcatcg
     2701 cctgaaaaga tgccgcaagt atccccggaa gcaactggag gccagtatga gaaggatgat
     2761 gttgtgggga tctatcctcg tggcgcttgc cgcaacccag
//
LOCUS       DASZYN010000786         1823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1079840, whole genome shotgun sequence.
ACCESSION   DASZYN010000786 DASZYN010000000
VERSION     DASZYN010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079840"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1823)
                     /locus_tag="VHE33_19915"
     CDS             complement(<1..>1823)
                     /locus_tag="VHE33_19915"
                     /inference="COORDINATES: protein motif:HMM:NF023218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HVT99776.1"
                     /translation="LRSPTDIRPGKAADTLADYFRPHPLTASVEISVDKDDTSATVTL
                     DHLELDVYHDDDPALQRTQDPAELLRPFWKGRRVTDEAVLLTSHNGEAATGTLMFRPA
                     RIVAVTSYDGATQYREGVDYTVQGRTLVAANSAIRQVRESDLLHGDLAWNVIGGKQIL
                     VTYEHDDAWTGPVQPYVGDQLPNTLRILRARQPLRIVACGDSITYGIGSSHMEKIPPY
                     QPPWVNLFAAELRRQWNDPGIALYNASQSGADSNWAKNMAGRMVASLHPDLVLVAFGQ
                     NDFWSVSADTFAANVSAVMATVRETNPQAEFLLVSTMRFDPAYSVNPEYWRRVTEYDA
                     RLRAMTAQGIQLVDMTAISGAVFAAKSPRDCLNDPLHPNDYLARWYAQSLVAALSPDH
                     EADFPNRKGVGDDDPEAPEAIDATGSHWYYNWKPAPSPGSIHAEFVPMLWGAGSVDAD
                     LRTAVQSGSKELLAFNEPDSETQGNVTVEQAIALWPKLEATGMRLGSPATTTGSPWLD
                     QFMAEAAARRLRVDFLCLHWYGDITAPDPVAALQKYLQGYWDRYHLPIWLTEYSGADF
                     SFHRRRTTVEDNARFAADSAAMLEKLPFVERYAWFGTAWTP"
BASE COUNT          332 a          562 c          585 g          344 t
ORIGIN      
        1 cggcgtccag gcggttccaa accacgcgta tcgttccacg aaaggcagct tctccagcat
       61 cgcggcggaa tccgccgcaa aacgcgcgtt gtcttccact gtggtgcggc gacgatgaaa
      121 cgaaaaatcc gcgccggagt actccgtcag ccagatgggc aggtggtagc gatcccagta
      181 gccctgcaga tacttctgca aagctgcgac cgggtccggc gccgtgatgt cgccgtacca
      241 gtgcaggcac agaaaatcca cacgcaggcg ccgggcagcg gcttccgcca tgaactggtc
      301 cagccacgga ctgccggtcg tcgttgccgg actgcccagg cgcatcccgg tggcctccag
      361 cttcggccac agcgcaatgg cctgttcaac cgtgacattg ccctgcgtct ctgagtcggg
      421 ctcgttgaac gcaagaagct ctttgcttcc cgactgcacg gcagtgcgca ggtcagcatc
      481 gacgctgccg gcaccccaca gcatgggaac gaactccgca tggatcgaac ctggggacgg
      541 cgccggcttc cagttgtagt accagtgcga tcctgtggca tcgatggctt ccggcgcctc
      601 cggatcgtca tcgcccacac ctttcctgtt ggggaaatcc gcctcgtggt ccggtgaaag
      661 cgcagccacc agactctgcg cataccaccg cgcaagatag tcgttggggt ggagagggtc
      721 gttcaggcaa tcccgcgggg acttggcggc gaagacggcg ccgctgatcg cggtcatatc
      781 caccagctga ataccctgtg cggtcatggc tcgcaaacgg gcatcgtatt ctgtcacccg
      841 tctccaatat tccggattga cgctataagc cgggtcgaaa cgcatcgtcg aaacgaggag
      901 aaattccgcc tgcgggttgg tctctcgcac ggtggccatg acggccgata cattcgcagc
      961 gaatgtatcg gcggagacgc tccagaaatc gttctgtccg aaggcaacca ggaccaggtc
     1021 cggatgcagc gaagccacca tgcgccccgc catgtttttg gcccagttgg agtccgctcc
     1081 gctctgcgac gcgttgtaga gggcaatacc gggatcgttc cattgcctgc ggagttcggc
     1141 ggcgaacagg ttgacccatg gcggctgata agggggaatc ttctccatgt ggctggagcc
     1201 gatgccgtag gtgatgctgt cgccgcaggc cacgatccgc agcggctggc gtgcgcgcag
     1261 gatgcgcagc gtgttcggca gctggtcgcc aacgtacggc tgtaccggac cggtccacgc
     1321 gtcgtcatgt tcataggtca ccaggatctg cttgccgccg atcacgttcc aggccagatc
     1381 gccgtgcagc aaatccgatt cgcgaacctg gcgaatggcg gagttggcgg ccacgagggt
     1441 gcgcccctgc accgtgtagt ccacgccctc gcgatactgc gttgcgccgt cgtagctggt
     1501 gaccgcgacg atgcgcgccg gacggaacat cagcgtgcct gtcgctgctt cgccgttgtg
     1561 ggaagtgagg aggactgctt catccgtcac ccgccgtccc ttccagaacg gccgcagcag
     1621 ttccgcagga tcttgtgtgc gctgcaaagc gggatcgtcg tcatggtaga cgtccagctc
     1681 gagatgatcg agggtgacgg tggcagaggt gtcgtctttg tcgacgctga tctcgacgct
     1741 ggcggtgaga ggatgcgggc gaaaataatc cgcgagcgta tcggccgctt ttccaggcct
     1801 gatgtcggtg ggcgagcgca ggg
//
LOCUS       DASZYN010000787         5714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_503872, whole genome shotgun sequence.
ACCESSION   DASZYN010000787 DASZYN010000000
VERSION     DASZYN010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503872"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..907)
                     /locus_tag="VHE33_19920"
     CDS             complement(122..907)
                     /locus_tag="VHE33_19920"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HVT99777.1"
                     /translation="MSLKTEQRAKKILQLLLQHGNASVDELVGYLGASAPSVRRDLAR
                     LEERNLVHRTHGGARLAGQMQYEPFRFDSSFHVRESRFTEEKRRIGVAAAELIREHET
                     VGFTAGTTPTQVARCIRHRNAIHVITNAVNIAMELSNQPALNVTLTGGTMRWAGAFSL
                     TGPTAMEMLGDVFMDKVFIGACGVDAKRGATTIEPDEAAVFRAMVRQAKQVIVVADSS
                     KIGMLSPALICAGSEIDILVTDAGITPDALEAFRANDIEVLAV"
     gene            complement(965..1732)
                     /locus_tag="VHE33_19925"
     CDS             complement(965..1732)
                     /locus_tag="VHE33_19925"
                     /inference="COORDINATES: protein motif:HMM:NF015866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HVT99778.1"
                     /translation="MQLEICVDSVESAIAAERGGADRVELCSDLLEGGITPSAGLMAL
                     VRKRIGIGVFVMIRPRGGDFCYTDAEYEVMREDIRQARELGADGIVLGVLDEHARVDV
                     ERTRKLVELAGSLPVTFHRAIDMTPDPCGALKDVIEAGATRVLTSGGAAKVTEGTSVV
                     ARMVAAAGDRIHVMAGGGITLETIARVAEATGAGEFHASLRSARPSPVDFHRRHVQMG
                     EVRDREYLRFVVEEEKVRALAGILQHLAETRPAAPRR"
     gene            complement(1844..5254)
                     /locus_tag="VHE33_19930"
     CDS             complement(1844..5254)
                     /locus_tag="VHE33_19930"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HVT99779.1"
                     /translation="MAKTLRQLMWLGALVLCASVPAAMAQNVTGSITGQVTDASGAVV
                     AGAQVIAHNLDTGVNTTATTNGEGIYRIDFLPAGHYQVTVDAKGFSKATLPEFALEAV
                     ETPTLNVKLQVGTATTTVNVSGAPPILNTNDPTLSATFTANTVSNFPLNGLDFSAVTL
                     YEPGVVDTAGTSGPTQIERSNYYVDTPNVNGNRAQANNYTLDGIDINESFNNLISYSP
                     APEALQEIKVLTANSPAQFGNVNGGGVLSILKSGTNAFHGSAYGYVQDYRLNANSWQN
                     NQARPIEPINPFSQAQFGGSLGGPIIRNKLFFFVDYLGSRYHKGGTGTASVLTAAMRG
                     GDFSALLTGANPIQLYDPENGFAPYTGDKGVAIVNPVAKFLFANPNYYPLPNAAPTDG
                     IINNNYQGPTRSYKANNQGDIKIEYDPRSADRITGFYSMSTAYDGSTAVLPITFPGVN
                     LFPTKVVGANWVHTFSASLINSAHIGFTRTDWNTGLPQDPTGQFGTGGNAKVGITFPN
                     QRFNGFSFQNIKNGPTGVGTPAFDGGIIDNTYSYMDDVTWQRGLHTLTFGVEAIRYQN
                     NYPTSNNNGYLGSLTYDGSFTSNPALPEAGGYSGADFVLDRVSSAAVTLNSVNVGQRQ
                     WRIAGYAQDDYKIRPRLTLNLGLRYEWDEPWVEENDKTGNINLSTGQIIYAGHVPTGA
                     PAGAGKCSNRGCYDSNFRQIMPRLGFAYQATDRFVVRGGYGATSFYEGNSSNQRLTSI
                     TPFIQAVNVTTVEPSAGNPGSSRTAEQGFGGGTVAYGGTFNVYPQNIQPAYVQEWNLT
                     LQYALTGTTSLQAGYVGESGQHIEDYGNVNQYRVNGDPTTAPFYNNQYIGINAVDPSV
                     SIGSNSLLITESRAKMNFNALESTLRQRLNHGLEATVNYTYSKAMTNSLGNYALNVNG
                     YSGAFQNYYDAAADYGPAGYDVKHNLSFTGVYALPFGRGQEYFAGTNRAADAVIGGWK
                     LSAAGVAWSGFPQTVTGPGNNSNSYGNSRPNQYRRPKIVNRNLSQWFGDDPSVTDVCP
                     SGVDDGICAFGIPATNSSGGTAFGTAKNGSLRGPGFKNVDLSAFKDFHTWHEQVVGFR
                     FDAFNAFNMVSYGNPDTGVTDSNFGAIAPLGQIRSQERRLQFSLHYSF"
     gene            complement(5474..>5714)
                     /locus_tag="VHE33_19935"
     CDS             complement(5474..>5714)
                     /locus_tag="VHE33_19935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99780.1"
                     /translation="VAALAGASGMEARVVDTPAQESRGEYRATWVLVSNNAAFFGQPE
                     VAALARKISAKAGLRAWTDDYSSLLPLLQWKVPGR"
BASE COUNT         1080 a         1999 c         1453 g         1182 t
ORIGIN      
        1 tagaacagcg caaccggttt cgttccatgc gcggccccgc cttcctgact cgcaaccgca
       61 ccctggctcg tcgccaccgc gaacatcgcc aacccagcca ccatcagacg aaccgtccat
      121 ctcataccgc aagcacctca atatcgttag cccggaatgc ctccagcgca tccggtgtga
      181 tccccgcatc cgtcaccaga atatcgatct cgcttcccgc acaaatcagg gcagggctca
      241 gcataccgat cttgctcgag tcagccacta caatcacctg cttcgcctgg cgaaccatcg
      301 cgcgaaacac tgcagcctca tccggttcga tggtcgttgc gccccgtttt gcatccacgc
      361 cacaggctcc aatgaatacc ttatccatga agacgtcacc gagcatctcc atcgccgtcg
      421 gccccgtgag cgaaaacgca cccgcccacc gcatcgttcc gcccgtcagc gtcacgttca
      481 gcgccggctg attgctcagc tccatcgcga tattcaccgc gttcgtgatt acgtgaatcg
      541 cgttgcgatg ccggatgcag cgcgccacct gcgtcggcgt tgtccccgcc gtgaaaccca
      601 ccgtctcgtg ctcgcgaatc agctccgcag ccgccacccc aatgcgtcgt ttctcctcgg
      661 taaaacgcga ttcccgcacg tgaaacgacg agtcgaaccg gaacggctca tactgcatct
      721 gtcccgccag ccgggctcca ccgtgggtcc gatgcacaag gttccgctct tccagacgag
      781 ccagatcgcg gcgaacactc ggagccgaag ctcccagata ccccaccagt tcgtccaccg
      841 acgcgttccc gtgctgcaaa agcagctgca ggatcttctt ggctcgctgc tccgttttca
      901 gtgacatggt gtcgccgaac cattatctat gaaactcagc gctcgtaagg tcacccgccc
      961 caccttaccg gcgcggcgcg gcaggtcgcg tctccgccag atgctgcaga atgccggcga
     1021 gcgcgcgaac tttctcctct tccaccacga atcgcagata ctcgcgatcg cgcacctcac
     1081 ccatctggac atggcgccga tgaaaatcca ccgggctggg ccgcgcgctc ctcaggctcg
     1141 cgtgaaattc gcctgcgcct gtagcttctg ccacacgcgc aatcgtctcc agcgtgatac
     1201 caccacccgc catcacgtgg atgcgatctc ccgcagccgc aaccatgcgc gccacaaccg
     1261 aagtcccctc cgtcaccttc gcggctccgc ccgaggtcag gacgcgcgtt gcccccgctt
     1321 cgatcacatc cttgagcgcc ccgcatggat ccggtgtcat atcgatcgct cggtgaaatg
     1381 tcacaggcaa cgaacccgcc agctccacca gttttcgcgt acgctccaca tccacgcgcg
     1441 cgtgctcgtc gagcacgccc agcacaatcc catccgctcc caactcccga gcctgccgga
     1501 tatcttcgcg catcacctca tattcagcat cggtatagca gaagtcgcca ccgcgcggcc
     1561 gaatcatgac gaacacgcca atcccgattc gctttcgtac aagcgccatc aatccagccg
     1621 acggcgtgat accgccctcc agcaggtcgc tgcacaactc cacccgatcg gcgcccccac
     1681 gttcggcggc aattgccgac tccaccgagt cgacacaaat ttcaagttgc ataagagccg
     1741 aatcccatct tccacaacat cccacgcgcc tttatacagc agacacaact atctgccgtt
     1801 aacggcgaag gaccgggctg ccgcctgtac agcggcagcc gaatcagaac gaatagtgca
     1861 gggagaactg aagacgccgc tcctgggaac gaatctgccc cagcggcgca atcgccccaa
     1921 agttgctgtc cgtcacgccc gtgtctggat tgccgtagct caccatgttg aacgcattga
     1981 acgcatcgaa gcggaatccc accacctgct catgccacgt gtggaagtcc ttgaaggccg
     2041 acagatcgac atttttaaat ccgggcccgc gcagcgagcc gttctttgcc gtgccgaatg
     2101 ccgtcccgcc cgaactgttc gtcgccggaa tgccaaaggc gcagatccca tcgtccacgc
     2161 ccgaggggca tacatccgtt accgatggat cgtcgccaaa ccactgcgac agattccggt
     2221 tgacgatctt cggcctccga tactgattcg gccgcgaatt gccgtagctg ttcgagttgt
     2281 tgcccgggcc cgtcaccgtc tgcggaaatc ccgaccacgc gacgcctgca gccgagagct
     2341 tccagccgcc gatcaccgca tcggccgcac gattcgtgcc ggcgaaatat tcctgtcccc
     2401 gcccgaaggg cagcgcatac acacccgtaa acgaaaggtt gtgcttcacg tcataccccg
     2461 ccggaccgta gtccgcggcc gcgtcgtagt agttctggaa cgcgccgctg tacccgttca
     2521 cgttgagcgc gtaattcccc aggctgttcg tcatcgcctt gctgtacgtg tagttcaccg
     2581 tggcttccag cccgtgattc agccgctgcc gcagcgtcga ctccagcgcg ttgaagttca
     2641 tcttcgcgcg cgactccgtg atcagcagcg agttcgaccc gatgctcacc gaaggatcga
     2701 ccgcattgat cccgatgtat tgattgttgt agaacggcgc tgtcgtcgga tcgccattca
     2761 ctcggtactg attcacgttg ccatagtctt cgatgtgctg ccccgactcg cccacatacc
     2821 cggcctgcag cgacgtcgtg cctgtcaacg catactgcag cgtcagattc cactcctgca
     2881 catacgccgg ctgaatgttc tgcggataga cgttgaatgt tcctccgtaa gccaccgtcc
     2941 ccccgccaaa cccctgttct gcagttcggg agctgccggg gttccccgct gatggttcca
     3001 ccgtcgtgac gttcaccgcc tgaatgaatg gcgtgatcga tgtcagccgc tggtttgagg
     3061 agttgccctc atagaaactc gtcgctccgt atcctccgcg aaccacgaag cgatccgtcg
     3121 cctgatatgc aaagcccagc cgcggcatga tctgccggaa gttcgaatcg tagcagccgc
     3181 ggttgctgca ctttcccgcg cccgccggcg ctcccgttgg aacatgcccg gcatagatga
     3241 tctgtcccgt gctcaggttg atgtttcccg tcttgtcgtt ctcttcaacc cacggttcgt
     3301 cccactcgta acgcaatccc agattcagcg tcagccgcgg acgaatcttg tagtcatcct
     3361 gcgcgtatcc cgcgatgcgc cactgccgtt gccccacgtt cacgctgttc agcgtcaccg
     3421 ctgccgaact cacccggtcc agcacaaaat cggcgccgct gtatccacct gcttccggca
     3481 gagccggatt gctcgtgaac gagccgtcgt aggtcagcga ccccagatac ccgttgttgt
     3541 tgctcgtcgg atagttgttc tggtaccgga tcgcctctac tccaaacgtc agcgtgtgca
     3601 gcccgcgctg ccacgtcaca tcgtccatgt agctgtacgt gttgtcgatg atgccgccat
     3661 caaaggccgg cgtccccacc ccggtaggtc cgttcttgat gttctggaac gaaaacccgt
     3721 tgaatcgctg attcggaaaa gtgatcccta ctttcgcgtt gccgcccgtc ccgaactgcc
     3781 ccgtcggatc ctgtggcaat cccgtgttcc agtcggtccg cgtgaaaccg atgtgcgctg
     3841 agttgatcag cgacgcggaa aacgtgtgga cccagttcgc gccaactacc ttcgtcggaa
     3901 acagattgac tccgggaaat gtaatcggca gcaccgccgt tgacccgtcg taggccgtcg
     3961 acatcgagta gaaccccgtg atgcggtccg cactcctggg atcgtattcg atcttgatat
     4021 cgccctgatt gttcgctttg taactccgcg tcggcccctg gtagttgttg ttgataatcc
     4081 catcggtggg cgccgcattc ggcagcgggt agtaattcgg attcgcaaaa agaaactttg
     4141 ccaccgggtt gacgatcgct acgcccttgt cgcccgtata aggcgcaaac ccattctccg
     4201 gatcgtagag ctggatcgga ttcgcacccg tcagcagcgc cgagaaatcc ccgccgcgca
     4261 tcgccgccgt cagcacgctc gccgtccccg ttcccccttt gtggtaacgc gacccgagat
     4321 aatcgacaaa gaagaacagt ttgttcctga tgatcggccc gcccaggctt ccgccgaact
     4381 gtgcctgcga gaagggattg atcggctcaa tgggtcgcgc ctgattgttc tgccacgaat
     4441 tcgcattcag ccggtagtcc tgcacgtatc cgtacgccga gccgtgaaac gcattcgtcc
     4501 cgctcttcag aatgctgagc acgccgccgc cattcacgtt cccgaactgc gcgggcgagt
     4561 tggccgtcag caccttgatc tcctgcaagg cctccggggc cggactgtac gaaatcaggt
     4621 tgttaaagct ctcgttaatg tcgatcccgt ccagcgtgta gttgttcgcc tgcgcgcggt
     4681 tgccgttcac attcggcgtg tccacgtaat aattgctgcg ctcgatctgc gtcggacccg
     4741 acgttcccgc cgtgtccacc acccccggct catacaacgt caccgccgag aaatccaggc
     4801 cgttcagagg aaaattcgaa accgtgttcg ccgtgaacgt cgcgctcagc gttggatcgt
     4861 tggtgttcag aatcggcggc gcccccgaca cattcactgt cgtcgtcgcc gtcccgacct
     4921 gcagcttcac gttcagcgtc ggtgtctcca ccgcttccag cgcaaactcc ggcagcgtcg
     4981 ccttgctgaa gcccttcgcg tcgaccgtca cctggtagtg cccagccggc aaaaagtcga
     5041 tccggtagat cccctcgccg ttcgtcgtcg ccgtcgtgtt cactccggta tccagattgt
     5101 gtgcgatgac ctgggctccc gccaccactg ccccgctcgc atcggtcacc tgtcctgtga
     5161 tcgatccggt cacattctgc gccattgccg ctggaacgct tgcgcacaac accagcgcgc
     5221 ctagccacat caactgcctc aaagttttcg ccattacgtc tctccccctc gattcatttg
     5281 tcgtcggatc gtccagggtt tctgcctgca tagcggcagg cgtcgggctc gctcttcgcc
     5341 cgatgcgaac ataattcaac catatttatg ttgttttaac cattaaaatg atcaaagtgg
     5401 tcaagtagtg acgaaacacg cctcgcccgt gaggagtcca gcaaagccaa ccctctggat
     5461 tttcagtacc tgatcagcgt cctgggactt tccactgcag gagcggcagc aggctcgaat
     5521 agtcatccgt ccacgcgcgc aagcctgcct tcgctgaaat cttccgcgct aatgccgcca
     5581 cctccggctg cccgaagaac gcagcgttgt tcgacaccag cacccacgtc gcccggtatt
     5641 cgccgcgcga ctcctgtgcc ggtgtatcca ccacgcgcgc ttccattccc gacgccccag
     5701 ccagtgccgc cacc
//
LOCUS       DASZYN010000788        10920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_690307, whole genome shotgun sequence.
ACCESSION   DASZYN010000788 DASZYN010000000
VERSION     DASZYN010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 10920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10920
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690307"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VHE33_19940"
     CDS             complement(<1..434)
                     /locus_tag="VHE33_19940"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HVT99781.1"
                     /translation="MYPFIHIGRFSIGTFGIMLWLAAVAGCWVLQRNFRRWKIDADAI
                     SIVAVVTVAGVIGSKLWHVLETPSALMAHPADLLFDRAGFAWYGGLVVGILTLLWQAR
                     VYKLGSLQMLDLASPAAAIGYGVGRLGCLVSGDGDYGIPTKLP"
     gene            489..1049
                     /gene="hpt"
                     /locus_tag="VHE33_19945"
     CDS             489..1049
                     /gene="hpt"
                     /locus_tag="VHE33_19945"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HVT99782.1"
                     /translation="MSTTRTSTALEVLFSREQISRRVAEMGRQIDQDYAGDKVVLVGV
                     LKGAAIFLADLARSISVDNTFDFVAVSSYGKGSRTSGAVKLIKDLDNPIENKNVIIVE
                     DILDTGLTLHFLRSLFEQHQPKTLRIAALLDKPSRRVETIEADYVGFTIPNQFVIGYG
                     MDFAERYRNLPDICLMPPDSPELSAG"
     gene            1066..1818
                     /gene="deoC"
                     /locus_tag="VHE33_19950"
     CDS             1066..1818
                     /gene="deoC"
                     /locus_tag="VHE33_19950"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HVT99783.1"
                     /translation="MTPIHLIDEEEEQEIAGFDAEAFAQRTLGSKQNLAAVIDHTLLK
                     PEATQEQVIRLCREAAEHRFACAMVNPAWAALAWSELAGTGIPVGVVVGFPLGASLGT
                     TKRDEAAALVKIGAHDIDMVLNVGLLKSGENQAVEQDIAGVVEVVHDAGAIVKVILET
                     CLLSVEEKLRASELAIAAGADFIKTSTGFSTGGATPEDVGLMRGVAGGRCGVKASGGI
                     RTLADARRMLEAGANRIGASAGVAILGELQPA"
     gene            1956..3899
                     /locus_tag="VHE33_19955"
     CDS             1956..3899
                     /locus_tag="VHE33_19955"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HVT99784.1"
                     /translation="MTEDSQQNPAEHEWEGDYRPVSPEASQRPAQVQVDPLQTEFLIR
                     LADTLNTTLDLRTLLERTASLVRAVIDYRIFSILLFNDRTNDLRMRFQIGHKPEVERM
                     RIRMGQGIVGEVAQSRQPMLINDVSKTGNYINANPDVRSELAVPLITKNRMIGVIDIQ
                     AEQLNYFKQEHLHLLTLTASRIAGAIENARLYTRVARQAQTLQVLNEISREITSILDP
                     DELLQRVGQLVRRIIDYQMFSVWLVNERDQVLENRLSIRFTHRSESKTKEKENLPEEP
                     KRTIWDSSPPIVPQLAPPSSVLGLAENSGVIGTTIPIDRGLVGAAIREKRVIHVADVR
                     KDSRYHMVNPETRSEMTVPLIYKGTAIGVLDIEHTRLHYFTEDHERAMTTLAAQIAIA
                     LENAQLYQRVAQQEQRLERDLAMAREVQLRLLPPAKPQHKRAEFAARFLPARTIGGDL
                     YDFLQYDPNRSAIALGDVSGKAAPAALYAALVSGIMRSVASRHLSPSEMLRTLNDALQ
                     ERRLDSQYVTMLYAVWNDENLSVQIANGGSVQPLFCRGEEIETIKAEGFPLGMFPNAT
                     WEEFSLSTQPGDSVVFFSDGIVDAQNSAGEMFGTERLVATVKKHQHKSASRLAESILT
                     DVGRFQGKRERFDDETVVVLRVI"
     gene            4268..4504
                     /locus_tag="VHE33_19960"
     CDS             4268..4504
                     /locus_tag="VHE33_19960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99785.1"
                     /translation="MNEISKLLQERFGLSPDQAQEAERLILGLIRSKIPAQFQGIADS
                     LLGSSPAPGAEPSQAAAGSGNSLLSAAEGLLHRG"
     gene            4590..4937
                     /locus_tag="VHE33_19965"
     CDS             4590..4937
                     /locus_tag="VHE33_19965"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HVT99786.1"
                     /translation="MKMPRRIASALLALAACSLFSVLPAFSQAKPGAKPSATSAAASA
                     GKAAVTLLDINSATADQLKALPGVGDVYSQKIIAGRPYANKTQLKSKGIIPAATYDKI
                     ASLIIAKQPAKSH"
     gene            5116..5553
                     /gene="rbfA"
                     /locus_tag="VHE33_19970"
     CDS             5116..5553
                     /gene="rbfA"
                     /locus_tag="VHE33_19970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HVT99787.1"
                     /translation="MPEQRGRKYHQERVAETLRDEIGAMIEGELSDPRISFAYVTQVV
                     LNPGGKSALIYVAVDGGAQEEADTLEGLMAARGYIRHELLERMGKRYVPELTFHIDRS
                     EKMKARIDDLLGRKRQARLRAQNSAPDSAAGPLQPQKTQDSGK"
     gene            5553..6602
                     /locus_tag="VHE33_19975"
     CDS             5553..6602
                     /locus_tag="VHE33_19975"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HVT99788.1"
                     /translation="MAAGHDIPLPHDDDRPNAGREALRDGLDAVLNVIHRARRFAVCS
                     HARPDGDAIGAMLGCAMILEQLGKTAAMVSADPVPMIYRGLPCAGAIRHVHRIEGEYD
                     AVILLECDGIQRSRIGGLEGRFLVNIDHHATGRPFGDVNWIDTGACAVAEMIYRLAIA
                     ARAEVTPQIATCLYAGVLTDTGSFCYEGTDAHAFEVARELVGRGADPAGIAREVYFSN
                     PLSKMLLLGAALSNLRREGRLAWLWVTSEDMTRTGAAEEDCEGIVNYAIGIAGVEGAV
                     LLRELPDRRVRLSLRSKGALNVSRIAASLGGGGHLHAGGCTVDGPLRQATEQILHTMR
                     QELMIMVPGRAAAKE"
     gene            6741..8906
                     /locus_tag="VHE33_19980"
     CDS             6741..8906
                     /locus_tag="VHE33_19980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HVT99789.1"
                     /translation="MTETPKSSAKTEPSSGDRSGAAAAERSVAPAVSSRTRGRASDDG
                     GKRGLFLRFRSRFPAIGEILEAATLFCLTAFFLFYGLVPIFGGDGLGLVGADEPRYAQ
                     VAREMLTRHDYVTPVLWGHPWLEKPALYYWRAMFAFREFGVHDWSARLPSASFAFMLV
                     VLIYLHIRRFRNGGQLDAALITACSAAILSFSRGASTDMQLAAPFCIGMLGWYAWYET
                     NSKFWLFDIYFFVGAATLAKGPIAPLMALVIIGIFAILRREWSLFRRSLWWPGIVLYF
                     AMVLPWFIEVQRRNPTFLRVFFLEHNLERFATNRFEHYHPFWYYVPVMLLGLTPWAVI
                     AVTAFVDAIGGSINEWKARRAKVLYVGHGRAGDAFPEFLVLWALVPIVFFSFSDSKLP
                     GYVLPAVPPLTILAGDYLNRIRNRGLKPWLLVLHAVLTGILSMFVLLMPLHLHNPEAI
                     PPRVAIIAAGMVGLATVVFILITVARFGLKRLRIATMTPMVILLLYIFGIGPLFGISP
                     AANTKHNINLMDMTYSARPLAQILNQIGPRPGIIAVYRVRRDVQYGLSFYRNQRTVNY
                     DVGITVSDPPPDGPGPHGVLIDEIKEGSSAEQMGLNRLIGDDLVAVNGQPVLNTADFQ
                     AMRARWKPGVRLEFEVLDPRIPKKGPQFARGILQDGVPDQQHLLVIPQACLSQVPCAS
                     ELHRNLAGRQYDPLFTYPAQNLVVYEVMASQSSKAVHTR"
     gene            8979..10046
                     /locus_tag="VHE33_19985"
     CDS             8979..10046
                     /locus_tag="VHE33_19985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HVT99790.1"
                     /translation="MSSAVDFELLDLRHFSASSLRPLLDEESRVWSRRLHWDYRGSAE
                     LLLQYLDSRVLPGYVAVENGRIAGYIFCVYEGYKAVIGDVFAASDPNAESDTQSVPAT
                     DDDATAEVRNELLLRLLEMLKSTPGVTRIESQLLLHPHGAHQQIFENAGFEVHERIFM
                     EWPLQPGLSTAARDGASPPTPENPSLPQGMVLRRWSEPDLSAAARLIASSYEGHLDSH
                     INEQYRTVSGSQRFLHNIVRFPGCGYFDADASLALVEPGSTELAGLLLCSRVRDDVGH
                     VTQICIAPQHRGLGLGRLLMVECARSLAKRGFSLLTLTVTASNRKAVSLYEWLGFRGT
                     HRFDAMVWDANEDPASRNKRR"
     gene            complement(10071..10724)
                     /locus_tag="VHE33_19990"
     CDS             complement(10071..10724)
                     /locus_tag="VHE33_19990"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HVT99791.1"
                     /translation="MIQVITVEREYGSQGAEYAHHLAHRLGWRLIDGCLTEDVARKAG
                     VTPTLVKRCDERVDPWFYRFGKAFWLGSLETLPEDPKVFDSARMVEFVKGYVQEQAAI
                     GSCVVVGRGAACMLARSPGAYHVFVYASMARKIRYIQEKFPEHSSDAENEIIATDRRR
                     AEYIRRFHQREWDDRKLYHLMMNSCMGQHAMVEATVEAAGLRSVADAVEHAEQAAVG"
BASE COUNT         2069 a         3691 c         3037 g         2123 t
ORIGIN      
        1 ggcagcttcg tggggatgcc gtaatcgccg tcgccggaca ccaggcagcc cagccgcccc
       61 acgccgtagc caatcgccgc cgccggcgaa gccagatcca gcatctgcag ggaaccgagc
      121 ttgtaaacgc gcgcctgcca cagcagcgtc agaatgccga cgaccaggcc gccgtaccag
      181 gcgaatccgg ctcggtcaaa cagcaggtcc gcgggatgcg ccatcagagc actcggcgtt
      241 tccagcacgt gccatagctt cgatccaatg accccggcca cggtcacgac ggcgacaatg
      301 ctgatcgcat ccgcgtcaat cttccagcgc ctgaaattcc gttgcagaac ccagcagccg
      361 gccaccgcag ccagccaaag catgatgccg aatgtgccaa tggagaaacg gccgatgtga
      421 atgaaagggt acatacgtgc gtctactgag tatagcttcc acagggggct ttcgaggtaa
      481 tctgacctgt gagcacgacg cgcacgagca ctgcccttga ggttcttttc agccgcgagc
      541 aaattagccg ccgtgtagcc gagatgggtc gccagatcga tcaggactac gccggcgaca
      601 aggttgttct cgtcggcgta ctcaaaggag cagccatctt cctggccgat ctcgcccgca
      661 gcatttcggt cgacaacacc ttcgatttcg tggctgtctc cagctacggc aaaggctccc
      721 gcaccagcgg agccgtcaaa ctcatcaagg atctcgacaa tcccatcgag aacaaaaacg
      781 tcatcatcgt cgaagacatt ctcgatacag ggctcacgct ccacttcctg cgcagcctgt
      841 tcgagcagca ccagccaaaa accctccgca tcgccgccct gcttgacaag ccttcgcggc
      901 gcgtggaaac aatcgaagcc gattatgtgg gattcaccat tcccaatcag ttcgtgattg
      961 gttatggaat ggatttcgcg gagcgctacc ggaaccttcc ggacatctgt ctcatgcctc
     1021 ctgattcccc ggaattatcg gcgggctgaa aggagttttg cccccatgac acccattcac
     1081 ctcatcgacg aggaagaaga acaggaaatt gccgggtttg acgcagaggc tttcgcgcag
     1141 cggacccttg gatcgaagca aaacctcgca gccgtgatcg accacaccct gctcaagccg
     1201 gaagccactc aggaacaggt gattcgcctt tgccgcgagg cggcggaaca tcgcttcgcc
     1261 tgtgccatgg tgaatcccgc ctgggccgca ctggcctggt cggaactggc cggtaccggc
     1321 atccccgtcg gcgtcgttgt cggctttccg ctgggggctt cgctcggcac caccaagcgc
     1381 gacgaagccg ccgcgctggt gaaaatcgga gcccacgaca tcgatatggt cctgaacgtc
     1441 ggcctgctca aatccggtga aaaccaggcc gtcgaacagg acatcgccgg cgtggtcgaa
     1501 gtcgttcacg atgcaggcgc catcgtgaaa gtgattctcg aaacctgcct gctgtcggtc
     1561 gaagaaaagc tccgtgcttc ggaactcgcc attgctgccg gcgcggactt catcaagacc
     1621 agtaccggat tctcaaccgg gggcgcgact cctgaagatg tgggactcat gcgcggggtg
     1681 gcgggtgggc gttgtggagt gaaagcctcg ggtggcattc gaaccctggc cgacgccagg
     1741 cgcatgctgg aagccggcgc gaaccgcatc ggcgccagcg ccggcgtcgc cattctcgga
     1801 gaactccaac ccgcctgatc ctgccggact gcgggctctg caaaccttgg tccagggggt
     1861 tgcacggcca ttccgcccgc aaacgcaggg ctgcgcaaaa ccccgtatca tggcatgggt
     1921 tcgcgtgcac accagccgcg tgctccagga gagttgtgac cgaagattcc cagcagaacc
     1981 cggcagagca cgaatgggaa ggagactatc ggcccgtctc tccggaagcc tcgcagcggc
     2041 ccgcgcaggt ccaggtcgat cctctccaga ccgaatttct catccgcctg gccgacaccc
     2101 ttaacaccac cctcgatctg cggactctgc tggagcgcac cgcctcgctg gtccgcgccg
     2161 tcatcgatta ccgcatcttc tccatcctcc tcttcaacga ccgcaccaac gacctgcgca
     2221 tgcgctttca gatcggtcac aagcccgagg ttgagcggat gcgcatccgc atggggcagg
     2281 gaattgtcgg cgaagtcgcg caaagccgcc agcctatgct gatcaacgac gtcagcaaga
     2341 ccggcaacta catcaatgcc aatcccgacg tccgctctga actcgccgtt ccgctgatta
     2401 ccaaaaatcg catgatcggc gttatcgata tccaggcgga gcagctgaat tacttcaagc
     2461 aggaacacct ccacctgctc accctcactg cctcgcgtat cgccggcgcc attgagaatg
     2521 cacgcctcta cacgcgcgtt gcccgccagg cgcagactct ccaggttctg aacgaaatca
     2581 gccgcgagat cacctccatt ctcgatcccg acgagctcct gcagcgcgtc ggccagctgg
     2641 tccgccgcat catcgactat cagatgtttt ccgtctggct cgtgaacgag cgcgatcagg
     2701 tcctggagaa tcgtctctcc attcgcttca cccaccgctc cgaatcaaaa accaaagaga
     2761 aagagaatct ccccgaggag ccgaagcgaa cgatttggga ttcgtcgccg ccaatcgtcc
     2821 cgcaactcgc tccgcccagc tccgtcctcg gtcttgccga gaattccggc gtcattggca
     2881 ccaccattcc catcgaccgc ggactggtgg gggccgccat ccgcgagaaa cgcgtcatcc
     2941 acgtcgccga tgtccgcaag gattcccgct accacatggt gaatccggaa acgcgctcgg
     3001 aaatgaccgt gcccctgatc tacaaaggca ccgccatcgg cgtgctcgac atcgagcaca
     3061 ctcgtctcca ctacttcacc gaagatcacg aacgcgccat gaccacgctg gcggcgcaga
     3121 ttgccatcgc tctggaaaac gcgcagctct accagcgcgt ggcccagcag gagcagcgcc
     3181 tggagcgcga tctggccatg gcccgcgagg tccagttgcg cctgctgccg cccgccaaac
     3241 cgcagcacaa gcgtgccgag ttcgccgccc gctttttacc ggcgcggacc atcggcggcg
     3301 acctctacga tttcctgcag tacgacccca accgcagcgc catcgcgctt ggcgatgtca
     3361 gcggcaaggc cgcgcccgcc gcactctatg ccgcgctggt cagcggcatc atgcgctcgg
     3421 tggcgagtcg ccatctgtct ccctccgaaa tgctgcgaac cctcaacgat gccctgcagg
     3481 agcgccgcct cgactcgcag tacgtcacca tgctctacgc ggtgtggaac gacgaaaacc
     3541 tcagcgtcca gatcgccaac ggaggatccg tccagcccct cttctgccgc ggcgaggaga
     3601 tcgagaccat caaagccgaa ggcttcccgc tcggcatgtt tcccaatgcc acgtgggagg
     3661 agttcagcct ctccacccag cccggcgatt cggtggtgtt tttcagcgac ggcattgtcg
     3721 acgcgcagaa ttccgccgga gagatgttcg gaacagagcg cctggtcgct acggtgaaga
     3781 aacaccagca caagtccgcc tcgcgcctgg ccgaaagcat cctgaccgat gtcggccgct
     3841 ttcaaggtaa gcgcgaacgc ttcgacgacg aaaccgtggt cgtgctgcgc gtgatctagt
     3901 gtggtgtttc tgaagttcct ttcccttgtc atcccgaccg gacctagcga aggggaagcg
     3961 gagggacctg cctctcttcc gcataaagga atttcggaaa caccacactc gcaacgatca
     4021 tcgtttcctg cgtgccattc ccaacgcggc gccgttgcag ccgtcgggat ttttacttgc
     4081 acaatcgggt aagggcattt atacaggttg cgccgggtgg cgggaccctc tcggctccgt
     4141 ccaccggctc atactgcggt gctggatggc tcagctgaat ccagccgaag ccccacccct
     4201 ctcgagccct gatccttctg ccgcgctgga gagcaagatc ccacaaggag tgaaaggaca
     4261 atcgcctgtg aatgaaatct caaagttgct gcaggaacgg ttcggcctca gcccggacca
     4321 ggcccaggaa gcagagcgcc tcattctggg tttgattcga tcgaaaattc cagcgcagtt
     4381 ccagggaatc gccgattcac tgctcggctc cagcccggcg cccggtgcgg agccatcgca
     4441 ggcggccgcc ggatcaggca atagcctgct gagcgccgct gagggcctcc ttcatcgcgg
     4501 ataagaggcc gcttctcatc ggtgatccgc acgaaactcg atcgcagact tcacggctca
     4561 cacccgccac ccacgaaagg aaacacgtca tgaaaatgcc acgtcgtatc gcgtctgctc
     4621 ttttagctct ggcagcctgc tccctcttca gcgtcctgcc cgccttttct caggcaaaac
     4681 cgggtgccaa acccagcgca acctctgccg ctgcgtccgc cggcaaggcg gccgtgactc
     4741 tgctggacat caactccgcc acagccgatc agctcaaggc gcttcccggc gtgggggacg
     4801 tctattcgca gaagatcatc gcgggtcgtc cttatgccaa caagactcag ctgaaaagca
     4861 aaggcatcat ccctgccgcc acctacgata agatcgcgag cctcatcatc gccaaacagc
     4921 ccgccaagtc tcactgagcg gtgcgcacaa aaaaggcgcc cgctcgggcg ccttttttgt
     4981 tttgatccag gcctctgcat caatttcagc ctgagccggt atccctgttc aacgatcgag
     5041 ttccgtctga tattctcaag ggttttatcc cgtatctgtg tcgatgccct gcaggcgggt
     5101 gggagggatg aggccatgcc ggaacagcgc ggacgaaaat accatcagga gcgggtggcc
     5161 gaaacgctgc gcgacgaaat cggcgccatg atcgaaggcg agctctccga tccgcgcatc
     5221 agtttcgcct atgtcaccca ggtggtcctg aatcccggcg gcaagtcggc tctcatttac
     5281 gtcgccgtcg acggcggcgc ccaggaagaa gccgatactc tggaaggcct gatggccgca
     5341 cgcgggtaca tccgccacga gttgctggag cgcatgggca agcgctacgt gccggaactg
     5401 acgttccaca tcgaccgctc ggaaaaaatg aaggcccgca tcgacgattt actggggcgg
     5461 aagcgccagg cccgtctgcg agcgcagaac agcgcaccgg acagcgcggc cggtccactg
     5521 cagccacaaa agacccagga ctcggggaag taatggcggc tggacacgac atcccgttgc
     5581 cgcacgatga cgaccggccg aatgcggggc gtgaggcgct gcgcgatggt ctggatgccg
     5641 tgctgaacgt gatccatcgc gcccgccgct tcgcggtttg ctcccatgcg cgccccgatg
     5701 gagacgctat cggcgccatg ctgggctgcg cgatgatcct ggagcagttg gggaaaactg
     5761 cggccatggt ttccgccgat ccggtcccga tgatctatcg cggtttgccg tgcgcaggcg
     5821 ccatccgcca cgtgcatcgg atcgaaggcg aatacgacgc ggtcatcctg ctcgaatgcg
     5881 acggcattca gcgctcccgc atcggcggcc tcgaaggccg tttcctcgtc aacatcgatc
     5941 accatgccac cggccgtccc ttcggcgacg tcaactggat cgataccgga gcctgtgcgg
     6001 tggcagaaat gatctatcgc ctggccatcg ccgctcgcgc cgaggttacg ccgcagatcg
     6061 ccacctgcct ctatgccggt gtgctgaccg acactggatc cttttgttat gaaggcaccg
     6121 atgcccatgc cttcgaggtt gcgcgagaac tcgtgggtcg cggggctgac cccgccggca
     6181 tcgcccgcga ggtctacttt tccaatccgc tgtcgaaaat gctgttgctc ggcgccgccc
     6241 tctccaacct gcgtcgcgaa ggccgcctgg cctggctttg ggtgaccagc gaggacatga
     6301 cccgcaccgg cgccgccgag gaagactgtg aaggcatcgt caactatgcg atcggcattg
     6361 ccggcgtcga aggagccgtc ctcctgcgcg agctgcccga tcgccgcgta cgcctctccc
     6421 tgcgcagcaa gggcgctctc aatgtgtcgc gcattgccgc cagtctcggg ggcggcggtc
     6481 atctccatgc cggcggatgc accgtcgatg ggcctctccg ccaggcgacg gaacaaattc
     6541 tgcacaccat gcgccaggaa ctgatgatca tggttccagg acgcgccgcc gcaaaggaat
     6601 aaccccttga gaatctgtta ggctgactca gtcgtggtgc atgcacatct tttggctgaa
     6661 cattcctgcc agacgcacct ttgcgggttg cgagtaaacg ccaatagcgg ctatcaacat
     6721 ggacggacac gtgcccgaac gtgactgaga ctcccaaatc ctcagcgaaa actgagccga
     6781 gttccggcga tcgctccggc gctgccgcgg cagaacgttc cgttgccccg gccgtctcgt
     6841 cgcgaacacg cgggcgtgcc agcgacgatg gaggaaaacg cggactgttc ctgcgttttc
     6901 gatcccggtt tcccgccatc ggcgaaatcc tcgaagctgc aaccctcttt tgcctgacgg
     6961 cgtttttcct cttctacgga ctggttccga tcttcggagg cgacggactc ggactcgttg
     7021 gcgccgatga gcctcgctac gcccaggtgg cgcgcgagat gctcactcgc cacgactacg
     7081 tcacgccggt tctgtgggga catccctggc tggaaaaacc cgccctttac tactggcgcg
     7141 ccatgttcgc ctttcgcgaa ttcggcgtgc acgactggtc tgcccggctg ccttccgcca
     7201 gcttcgcctt catgctggtc gtgctcattt atctgcacat ccgccggttt cgcaacggcg
     7261 gccagctcga tgccgcactc atcaccgcct gttccgccgc catcctcagc ttttcccgcg
     7321 gcgcgtcgac cgacatgcag ctcgccgctc cgttttgtat cggcatgctc ggctggtacg
     7381 cctggtacga aaccaacagt aagttctggc tcttcgatat ctatttcttt gttggagccg
     7441 ccacgctggc caaggggccc atcgcgcccc tcatggctct ggtcatcatc ggcatcttcg
     7501 ccatcctgcg gcgcgagtgg tcgctgttcc gtcgcagctt atggtggccg ggaattgtcc
     7561 tctatttcgc gatggtcctc ccgtggttca tcgaggtcca gcgccgcaat cccaccttcc
     7621 tgcgcgtttt cttcctcgag cacaacctcg agcgcttcgc caccaatcgc ttcgagcact
     7681 accacccctt ctggtattac gtcccggtca tgctgctggg actcacgccc tgggcggtga
     7741 tcgcagtcac cgccttcgtg gacgccatcg gcggctccat caatgaatgg aaggcgcggc
     7801 gtgccaaggt cctctatgtc ggccatggcc gtgccggcga cgctttccct gaattcctcg
     7861 tcctctgggc gctggtcccg atcgtcttct tctctttttc cgactcgaag ttgcccggct
     7921 acgtcctgcc tgccgttccg cccctcacga ttctcgccgg cgactatctc aaccgcatcc
     7981 gcaaccgcgg ccttaagccc tggctgctcg ttctgcacgc cgtgctcacc ggcattctga
     8041 gcatgtttgt gctcctcatg ccgctccatc tgcataatcc tgaagccatt ccgccccgcg
     8101 tcgccatcat cgccgccggc atggtcggcc tcgccaccgt cgtctttatc ctcattacgg
     8161 tggcgcgctt tggactgaag cgcctccgca tcgcgaccat gacccccatg gtcattctgc
     8221 tgctctacat cttcggtatt ggtccgctct ttggcattag tccggcggcg aacaccaagc
     8281 acaacatcaa tttgatggat atgacctatt cggcccgccc gctggcgcag attctcaacc
     8341 agataggtcc gcggcccggc atcatcgccg tctaccgggt gcgcagagat gtacagtacg
     8401 gtctttcttt ctatcgcaat cagcgcaccg tgaattacga tgttggcatc acagtgagcg
     8461 atccaccgcc ggatggtccg ggtccgcacg gtgtcctcat cgacgaaatc aaagaaggat
     8521 cgtccgccga gcagatggga ctcaatcgcc tcatcggcga cgatctggta gcagtgaacg
     8581 gccagcccgt cctgaacacg gcggattttc aagccatgag ggcgcgctgg aagcccggag
     8641 tcaggctgga attcgaagtg ctcgatccgc gaattccaaa aaagggaccg caatttgcac
     8701 gcggcatcct gcaggatggc gttccggatc aacaacacct cctggtcatt ccgcaagcgt
     8761 gtctttctca ggtaccttgt gcaagcgagc ttcatcgaaa cctggcgggg aggcaatacg
     8821 atccgctctt tacctatccc gctcagaacc tggtcgtcta cgaagttatg gccagtcagt
     8881 catccaaagc tgttcacacc cggtgagccg ttggttgtac agagatgggg aggggattcg
     8941 cgaggcccgc gccgggacgc ctccgcatcc gcacagcgat gagttccgcg gtcgatttcg
     9001 agctcctgga tttgcgccac ttctccgcca gcagtctgcg gccgctgctg gatgaagaaa
     9061 gccgcgtctg gagccggcgc ctgcactggg actaccgcgg ctccgccgag ctcctcctgc
     9121 agtatctcga ttcccgcgtc ctgcccggct atgtcgctgt cgagaacggc cgcatcgccg
     9181 gctatatctt ctgcgtctac gaaggctaca aggccgtcat cggagacgtt ttcgccgctt
     9241 ccgatccaaa cgccgagagc gacacccagt ccgttcccgc aacggatgac gatgccaccg
     9301 cggaagtgcg gaatgagtta ttacttcgcc tgctggaaat gctgaagagc actcccggag
     9361 tcacgcgcat tgaatcgcag ctgctcctcc atccccacgg agcccatcag cagatctttg
     9421 agaacgccgg cttcgaggtc cacgagcgca tcttcatgga atggccgctg cagcctggtc
     9481 tgagtaccgc cgcgcgcgac ggagccagtc cccccacgcc tgaaaacccg tctcttcccc
     9541 agggaatggt cctccgccgc tggtccgaac ccgacctcag tgcggccgcc cggctcatcg
     9601 cctcctccta tgaaggccat ctggacagcc atatcaatga gcaataccgg acggtctccg
     9661 gatcccagcg cttcctccac aacatcgttc gctttcccgg ctgcggctat ttcgacgccg
     9721 atgcctcgct cgcgctggtc gaacccggca gcactgaact ggccggtctg ctgctctgtt
     9781 cccgggttcg cgacgatgtc ggtcacgtca cccagatttg catcgcgcct cagcaccgcg
     9841 gcctcgggct gggccgcctt ttgatggtcg aatgcgctcg ctccctcgcc aaacgcggat
     9901 tcagcctgct cacgctgacc gtgaccgcat cgaaccgaaa agctgtctcg ctctatgaat
     9961 ggctcggctt tcgcggaacc caccgcttcg acgccatggt ttgggacgcg aatgaggacc
    10021 ctgcttcccg gaacaaacga cgctgagtaa cagcgaccgg gatcgccggc ttatcccacc
    10081 gccgcctgct cggcgtgctc tacagcgtcc gccaccgacc gcagtccggc cgcttccacc
    10141 gtggcctcca ccatcgcgtg ctggcccatg cacgaattca tcatcaggtg gtaaagcttg
    10201 cggtcatccc attcccgttg atggaatcgc cggatgtact ctgcgcgccg ccggtccgtc
    10261 gcgatgatct cgttctccgc atcgctcgag tgctccggga acttttcctg gatatagcga
    10321 atcttccgcg ccatcgaggc ataaacaaag acgtgatacg ctcccggcga acgcgccagc
    10381 atgcacgccg cgccacggcc caccaccaca caactcccga tcgccgcctg ttcctgcacg
    10441 taccccttca caaattccac catgcgcgcg ctgtcgaaga ccttgggatc ttcgggaagc
    10501 gtctccagcg acccgagcca aaacgctttg ccgaagcggt agaaccaggg atcgacccgc
    10561 tcatcgcagc gcttcaccag cgtcggcgtt actcctgctt tgcgcgctac gtcttcggtc
    10621 agacagccgt cgatcagccg ccatcccagc cgatgggcca gatgatgcgc gtactccgcg
    10681 ccctggcttc catattcgcg ctcgaccgtg atcacctgaa tcattgccgt aatccttccc
    10741 tttgacagcc gatgttgcaa cttttcgacg ggcctggtca agcgataaga cgcgccgatg
    10801 agccatttcg atccgtttca ttggcatttt ctattgcgcc gcaaggcgcg tccgcctgga
    10861 cggctagtcc cggaactcca cgaccgttgc ccccgccccg ccttcgttct gtcctggctc
//
LOCUS       DASZYN010000789         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1080171, whole genome shotgun sequence.
ACCESSION   DASZYN010000789 DASZYN010000000
VERSION     DASZYN010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080171"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VHE33_19995"
     CDS             complement(<1..647)
                     /locus_tag="VHE33_19995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HVT99792.1"
                     /translation="MRQMTLPDGLTVFGLNPDETRFLHSEVFSERSYLQHGVELRDGD
                     CVFDVGANIGMATLFFHKERKDVRIFAFEPNPAAVACLRANIELHGVRARVFACGLLA
                     ESGMVEFTSYPANTVVSGFRADAQRQRETTRTYMINGGVAPHGADRFVEALFRKEEKF
                     ACPVRTVSEVVDEEQVASIDLLKIDVELSEREVLDGIREEHWQRIQQVVMEVTDK"
     gene            complement(650..>2115)
                     /locus_tag="VHE33_20000"
     CDS             complement(650..>2115)
                     /locus_tag="VHE33_20000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HVT99793.1"
                     /translation="RIAGWLQRGQRAERVGILASRSVETYAGVLGTLWAGAAYVPVNP
                     DTPEERLIRILETAQLDALIADAAGLSMLTDRVLAHAPRRILWGGKPGEKTGQAGSPA
                     TETFDQLPDEGPDAPVTVPDEGLVYILFTSGTTGTPKGVMIEAGSVVQFLRIAQDRCD
                     FRKSDRFSQMSELTFDASVLDMYSAWGVGASVHVVPKSQLIAPAKFIREHELTVWFGV
                     PSTGAMMERMKMLKPGSFPSLRVSIFAGEALPVATALAWKAAAPNSVVENFYGPTELT
                     VDCIAQKVDDPPLVTPNRGTVAIGRAFPWNEAAVVDADLRFVPPETDGELVVAGRQVA
                     RGYLGDPKLTAERFPTIDGRRWYRTGDLVRQDADGVFHHLGRIDNQVKVLGNRVELEE
                     VEAHVREITGSDAVAIAWPWEDGRAMGIAVFHCAAGVTRDTVREQMQKRVPIYMVPSR
                     IVELESLPMSSSSGKVDRKALQRMLEESGSPAAEKAR"
BASE COUNT          387 a          778 c          564 g          386 t
ORIGIN      
        1 tccttgtccg tcacttccat gactacctgc tggatccgct gccagtgctc ttcccggatt
       61 ccatccagca cctcgcgctc gctcagctcc acgtcaatct tcagcagatc gatgctcgct
      121 acctgctcct cgtccacaac ctccgatacg gttcgcaccg gacaggcaaa tttctcttcc
      181 ttgcgaaaca gcgcctccac aaagcgatcc gctccatgcg gcgcaacacc gccgttgatc
      241 atgtacgtcc gcgtcgtttc ccgctgccgt tgggcatcgg cacgaaagcc ggaaaccacc
      301 gtgttcgccg gataactcgt gaactccacc attcctgatt cagccaacag gccacacgca
      361 aacacccgtg cccgaacccc gtgcagttca atgttggccc gcaaacaggc aaccgccgcc
      421 gggttgggct caaaagcgaa gatgcgcaca tccttccgct ctttgtgaaa gaacagcgtc
      481 gccatcccga tgttggcgcc cacgtcgaag acgcaatcgc catcgcgcag ctcaaccccg
      541 tgctgcagat aggagcgttc gctgaacacc tcgctgtgca ggaaacgcgt ttcgtccgga
      601 ttcagcccaa agactgtcag accgtccggg agtgtcattt gtcgcatggt cagcgagcct
      661 tctctgctgc cggcgagccg ctctcttcga gcatccgttg cagcgccttg cgatcgactt
      721 taccgctgct gctgctcatc ggcaacgatt ccagctccac gatgcgcgaa ggcaccatgt
      781 agattggcac gcgcttttgc atctgctcgc gcaccgtatc gcgggtcact ccggcagcgc
      841 agtggaagac cgcgatgccc atcgcacgac cgtcttccca cggccaggct atcgctacgg
      901 catcgctgcc cgtgatctcg cggacatggg cttccacctc ttccagctcc acgcgattgc
      961 ccaggacctt gacctggtta tcgatccggc ccagatgatg aaagacgccg tcggcgtcct
     1021 gccgcaccag atcgccggtg cgataccaac ggcgcccgtc gatggtggga aatcgctccg
     1081 cggtcagctt cggatcgccg agatagccgc gcgccacctg ccgtcccgcc accaccagct
     1141 ctccgtctgt ctccggcggc acaaagcgca gatccgcatc caccaccgca gcttcgttcc
     1201 acggaaaggc ccgcccgatc gccaccgttc cgcggttggg cgtcaccagg ggcgggtcat
     1261 ccaccttttg cgcgatgcag tcgacggtca gttccgtcgg cccatagaag ttctccacga
     1321 cgctgttcgg cgcggccgcc ttccacgcca gagctgtcgc gaccggcagc gcctcacccg
     1381 caaaaatcga cacccgcagc gaaggaaacg atcccggctt cagcattttc atccgctcca
     1441 tcatggcgcc cgtcgacggc acgccaaacc acaccgtcag ttcgtgctcg cggataaact
     1501 tcgccggcgc gatcagctgc gacttcggca ccacatgtac cgacgcgccc acgccccacg
     1561 ccgaatacat gtccagcacc gatgcatcga aggtcagctc cgacatctgc gagaagcggt
     1621 ctgacttccg aaaatcgcag cggtcctggg caatcctgag gaactgcacc acgctgcccg
     1681 cctcgatcat cacgcctttg ggcgtgcccg tcgtgcctga ggtgaacagg atgtagacca
     1741 gcccctcatc gggcacggtg acgggcgcgt ccggcccttc atccggcagc tggtcgaagg
     1801 tctccgtggc cgggctgccg gcctggcccg ttttctcgcc cggcttcccg ccccagagaa
     1861 tgcgccgcgg tgcatgtgcc agcacgcgat ccgtcagcat cgacagtccc gccgcatcgg
     1921 caatcagcgc gtccaactgg gcggtctcca gaatgcgaat caggcgctct tccggcgtat
     1981 ccggattgac gggcacataa gccgcccccg cccacaacgt gcccagtacg ccggcatagg
     2041 tttccacact gcgcgatgca agaatcccga cacgctcggc tctctgcccg cgctggagcc
     2101 agcccgcgat ccgcc
//
LOCUS       DASZYN010000790         2374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_569080, whole genome shotgun sequence.
ACCESSION   DASZYN010000790 DASZYN010000000
VERSION     DASZYN010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569080"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1971
                     /locus_tag="VHE33_20005"
     CDS             <1..1971
                     /locus_tag="VHE33_20005"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT99794.1"
                     /translation="GCFSSEVVASSALTVRLPAGLGVAQASAQAVAWLTAEYALCEVG
                     GLKRGDRVLIHAAAGGTGLAAVHLCRKAGAEIIATAGSDRKRDWLRSLGIRDVYDSRS
                     IDFAKYIKGGVDLVLNSLAGESVDAGAALLRPGGRFLELGKSNLRSPEEMQQRWPGIR
                     YLPVDLTPLFAARSPWVKERLAAILNGTAEGTVPQLPVTLFDEAEIKAAFRFMSRAEQ
                     IGRILVTRRHPQRIRGTHLVVGGMRGIGLRLAAWLIRRGARSLVLVGRSAPGLEAQRL
                     IEQLEANGTVVHICLGDIADAAVARRAIDLAGPELRGVWHSAGILENAPLEQQTWDRV
                     RNVLRPKVDGAWNLHRLTLGFELDQFVLFSSWASIAGSHGQVNHCAANAFLDGLAHLR
                     RSQGLPALSINWGAWADTGAASDDDVERQLAHSGMRKMSPDAALEALLAALGSSEPQV
                     AIGLIDWPRYLAQRPGRMQQALYSRWIRQNIAGSTEMRSRKQEEPHGEKSRVSSADAL
                     AWKHLPPAAREPALIRILAEHVRRVLDLHRDEEVDPEMPLSDLGMDSLLAVELRNSLS
                     GALGRPFPSTLLFDYPNLRSLARHIEAQSSPSLQDVQEETPKESSTRHSDLPKHNQTV
                     LDLLDAIEHLSDDEVDSFLEQDERSLHGVKNG"
     gene            1968..>2374
                     /locus_tag="VHE33_20010"
     CDS             1968..>2374
                     /locus_tag="VHE33_20010"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HVT99795.1"
                     /translation="MSVEPGSGEFLERLQKLSPRRLALLAAELQRRLSASETPEPIAV
                     IGMACRLPGGADTPEAFWKLLADGIDAIEPVPADRWDADAFYDPRPGTPGKANTKWGG
                     FVRNIDLFDAGFFGISPREAVGMDPQQRMLLEVA"
BASE COUNT          448 a          727 c          728 g          471 t
ORIGIN      
        1 ggttgctttt caagcgaagt ggtcgcctcc agtgcgttga cggttcgact gccggcaggt
       61 ctcggcgttg cccaggcctc tgcacaagcc gtcgcctggc tgaccgccga gtacgcactc
      121 tgcgaagtcg gcggtttgaa acgtggagac cgtgttctga ttcacgccgc tgccggcggc
      181 accggtcttg ccgccgtgca tctgtgtcgc aaggcaggtg cagagatcat cgccaccgcc
      241 ggcagcgatc gaaaacgcga ctggctccgt agcctcggta tccgtgatgt ctacgattct
      301 cgttccatcg attttgcaaa atacatcaaa ggaggcgtag atctggttct gaactcgctt
      361 gcgggcgagt cggtcgacgc gggtgcagcc ttgctgcgtc cgggtggacg attcctggaa
      421 ctcggcaaat cgaatctccg ttcgcccgaa gagatgcagc aacggtggcc cggaattcgc
      481 tacctgccgg tcgatctcac tcctcttttt gctgcgcgat ctccctgggt gaaagagagg
      541 ctcgccgcaa ttctcaatgg cacagcagag ggaaccgttc ctcaactgcc agtcaccctg
      601 ttcgacgagg cagagataaa agctgcgttt cgtttcatgt cgagagcaga acagatcggc
      661 cgcatccttg ttacaaggag acatccacag cgaatccggg gtactcattt ggttgtcgga
      721 gggatgcgcg gcatcggcct ccggcttgcc gcctggctga tcagacgcgg agcgcgttct
      781 ctcgtccttg tgggccgcag cgctccaggc ttagaagcac agcgcctgat cgagcagttg
      841 gaggcgaatg gcacagttgt gcacatctgc ctgggagaca tcgctgacgc agctgtggcg
      901 cgacgtgcca tcgatctcgc cggcccagag ctccggggtg tatggcacag tgcaggcatt
      961 cttgagaatg ctccactcga gcagcagacc tgggatcggg tgcgaaatgt gctgcggccc
     1021 aaagtcgatg gtgcctggaa tctgcatcgg ctcactctcg gtttcgaact cgatcagttt
     1081 gtcctcttct cttcctgggc ttcgatcgcc ggctcgcacg gtcaggtgaa tcattgcgcg
     1141 gcgaatgcgt ttctggatgg gttggcgcac ttgcgccgct cgcagggcct accagcactg
     1201 agcatcaact ggggcgcctg ggctgacacc ggtgccgctt cggatgatga cgtcgaacgg
     1261 cagctcgctc attccggcat gaggaagatg tctcccgatg ctgcgctcga agcactcctt
     1321 gcggcactcg gcagcagcga gccacaagtt gccatcggcc tcattgactg gccgcggtac
     1381 ctcgcgcaac gaccaggcag gatgcagcag gcactctatt cacgctggat tcgtcagaac
     1441 atcgccggat ccaccgaaat gcgaagccgg aagcaggaag aacctcatgg cgaaaaatcg
     1501 cgtgtttcca gtgccgacgc gctggcctgg aagcacctcc ctcccgccgc gcgcgagccg
     1561 gcgctgattc gcattctcgc ggagcatgtt cgccgagtgc tggacctcca tcgggatgaa
     1621 gaggtcgatc cggagatgcc tctcagcgac cttggcatgg actctctgct ggccgtggaa
     1681 ctgcgcaaca gcctctccgg cgcccttgga cgtccgttcc cgtcgacgtt gctcttcgac
     1741 tacccgaacc tcaggagcct ggcccgtcac atcgaagcgc agagttcacc ctctctgcag
     1801 gacgttcagg aagagacccc aaaggagtcg tccacccgcc acagcgatct gccaaaacat
     1861 aaccaaacgg tgctggacct tctggacgcg atcgagcacc tctccgacga cgaagtggac
     1921 tccttcctgg aacaggacga gcgttcgctg catggagtga aaaacggatg agcgtcgaac
     1981 ctggatccgg cgaatttctg gagaggctgc agaagctctc tccgaggcgg ctcgcgctgc
     2041 tggcagcgga gttgcagcgg cggctctctg cgtccgaaac gccggaaccc atcgcggtca
     2101 tcggcatggc ttgtcgtctc cccggaggcg cagacacgcc tgaggcattc tggaaattgc
     2161 tcgcagacgg catcgacgcg atcgaaccgg tgcccgcgga tcgctgggat gcggatgcct
     2221 tctacgatcc gaggcccggc actccgggaa aggccaacac aaagtgggga ggatttgttc
     2281 ggaatatcga cttattcgat gccggcttct tcggcatctc gccccgcgag gcagtgggca
     2341 tggacccgca gcagcgcatg ctcctcgaag tggc
//
LOCUS       DASZYN010000791         3940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_749228, whole genome shotgun sequence.
ACCESSION   DASZYN010000791 DASZYN010000000
VERSION     DASZYN010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749228"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            432..2249
                     /locus_tag="VHE33_20015"
     CDS             432..2249
                     /locus_tag="VHE33_20015"
                     /inference="COORDINATES: protein motif:HMM:NF022840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HVT99796.1"
                     /translation="MRISRLTIAGFRGVSQADLRLGSHSILVGPNNVGKTTVIEAIAL
                     LFGRDRLVRELTEHDFFGSAPDQRSRITTVATLTDFEADDQTRHREWFGAERGIAVWL
                     APDGTLHPARTDPTWKLAVQIGFCARFNVDTLEAETIRFFVDDEADIGDPFDEENTVR
                     PVRGKALQELGFFLVSATRTWDRWISFTSELFRKVIAATGEVPAEAVRAERGRVWNPA
                     ERLENAAGISGIVANINRELTHLMSAPPELQLRLTSTDSAGVLQAVVPHFQQGASTPT
                     LPAARQGSGLASLQSLLLLMQFGAARKDKGQCFILGVEEPELHVPPSQQKRLVNRLNA
                     VCTQTVMTTHSPLVASMFPPEDVIFMRNVGGTLSGSPLSLGNAAPSNHEQHLFYGWRN
                     RLVAALMHEYVLVPEGVSDVAWLDALQTAIELRQDWKGVGNEPTRFGTFVGVAPTADA
                     KVAETYTVAKRVHSDVVCLVDGDPAGLGYVAALKALTPPPKVVFVWPDGWTTENVVGW
                     IADAKRAVALSQLGTALGATFGNGAVLKTHLERHKTYLPLHECAAEVLAGIPECRART
                     SDLLNDMADVVRGASGPRKHLVPWPQQSTATTSVWRLKP"
     gene            2198..3571
                     /locus_tag="VHE33_20020"
     CDS             2198..3571
                     /locus_tag="VHE33_20020"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HVT99797.1"
                     /translation="MAAAKHGHDERVAVEAVIRAFFEGPAGSGKTHQLIEEAVTASRE
                     VLLHPEQKLLALTFMNGARHRLNFRFGEKRELRGRFACLTFDSFAGTVARRRRALSRS
                     LPPVRHGRDTNDFDRTCIEAARLLELPEVANWVANSYPLVVVDEAQDLDAHRLRLLKA
                     LSEACCVVAAADEFQNLNDHVDSGSVVTWLRMANRPHALTRIRRTAQDGLLRAAGALR
                     SGTGMCHELAFEDGFQPCHAGDGIRVVSPPAKNSGALSWAVANELSALGNYAVILTPD
                     AKGKTIQQVLASVQTKAFNRNKRAGTTFGPFPLVWERREEDDAAEWLARFDGDGTLKL
                     RDVVDTVSGAQRPECRYICERLERARNIRGETTVTRERLTQVIRDVLRDAGHALPPSG
                     PGRRVMTIPRAKNREFGSVLVLWPHSVAGSPEHQRRLLYNAITRAKDRCSVVVFGQGR
                     TDKAPFS"
     gene            complement(3774..3850)
                     /locus_tag="VHE33_20025"
     tRNA            complement(3774..3850)
                     /locus_tag="VHE33_20025"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3814..3816),aa:Pro,seq:tgg)
BASE COUNT          708 a         1189 c         1315 g          728 t
ORIGIN      
        1 tgtcatccgt gagcaccttg agccagttgg cgatgtagga ggcatggtct tcgcgtggct
       61 cgagcgcgag gtcgaggtcg gcgcagagga aggcggcgcc cagttcggtc acgagctcct
      121 ccacgtcttc gagtttttcg atgtccttga ggacgttatg gcgaatcgga gcaggttact
      181 gtggggtggc cgggaggggt tccgggcggt cttgggtcta gccatcgtgt tcattccgtt
      241 gggtttaata cttttggggt gcctgccacg ggtggtcgag cttgaagaga aataggccac
      301 gcggagcttg gagtatccag aagttctgcc gcctcaacaa cattccggaa aatctgtaag
      361 ccggtccgct gcgggaggct cgcgcccgcg acggcgatac actgcaagcc tcaacagcag
      421 aggtgcaggg aatgcggatt tcccgtctga ccatcgccgg atttcggggc gtgtcccagg
      481 ccgatcttcg gcttggcagc cacagcattc tcgtcggccc gaacaacgtc gggaagacga
      541 ccgtcatcga ggcgattgcc ctgttgtttg gccgtgaccg gctcgttcgg gaactgacag
      601 agcacgattt cttcggcagc gcgcctgatc agcgaagccg catcaccact gtcgcgacgc
      661 tgacggattt cgaggcagac gaccaaacgc gacatcgtga atggttcggc gcggagcgtg
      721 gcatcgctgt ctggctcgcg cccgatggca cgctgcatcc cgcacgaacg gacccgacct
      781 ggaaactcgc cgtgcagatc ggtttctgcg cgcggtttaa cgtcgacacg ctggaggcgg
      841 aaaccatccg gtttttcgtc gatgacgagg ccgacatcgg cgatccgttc gacgaagaga
      901 acacggtgcg tccggttcgc ggaaaggcac tccaggaact cgggttcttc ctcgtgtccg
      961 cgacacggac atgggaccgc tggatctcgt ttacgtcgga gttgttccgg aaggtcatcg
     1021 ccgcaaccgg agaggtgccg gccgaggccg ttcgcgccga gcgcggtcgc gtgtggaatc
     1081 ctgcggagcg cctcgaaaac gccgcgggca tttcgggcat cgtcgcgaac atcaaccgcg
     1141 aattgacgca tctgatgtcg gcgccgcccg agctccaatt gcgattgacg tcgaccgaca
     1201 gcgcgggcgt attgcaggcg gtcgttccgc atttccagca gggggcttcg acgccgacac
     1261 tgcccgcggc gcgccagggc tcgggcctcg cgtcgctgca aagcctgttg ctgctgatgc
     1321 agttcggcgc agcgcgaaaa gacaaggggc agtgcttcat ccttggcgtc gaggagcccg
     1381 agctccacgt tccgccgtcg caacagaagc gcctcgtcaa tcggcttaac gccgtctgca
     1441 cgcaaacggt catgacgacg cattctccgc tcgtggcttc gatgttcccg cccgaagacg
     1501 tcatcttcat gcgcaacgtc ggcggcacgc tgtccggctc gccgctctcc ctgggcaatg
     1561 ccgcgccgag caatcacgaa cagcatcttt tctacggttg gcggaatcgg ctggtggcgg
     1621 cgctgatgca cgaatacgtg cttgtgcccg aaggcgtatc tgacgtcgcc tggctcgatg
     1681 cgctgcaaac cgcaatcgaa ctgcgccagg actggaaggg cgttgggaac gagcccacgc
     1741 gctttggcac attcgtcggc gtggcgccga ccgctgacgc caaggtggcg gaaacgtaca
     1801 cggtcgcaaa acgcgttcac agcgatgtgg tgtgtctggt tgatggggac ccggcgggcc
     1861 tcggctacgt tgcggccctg aaagcgctca cgccaccacc taaggttgtt ttcgtgtggc
     1921 cggatggctg gacgacggag aatgtcgtcg gctggattgc cgatgcgaaa cgggctgtgg
     1981 cgctgtccca gcttggaacc gcgctcggcg ccacgttcgg caatggtgcg gtgctcaaga
     2041 cgcatctcga gcggcacaag acgtacttgc ccctccatga atgcgctgcg gaagtcctcg
     2101 ctggtattcc cgagtgtcgc gcccggacgt cggacttgct gaacgacatg gcggacgtcg
     2161 tgcgtggagc gagtggtccg cgcaaacatc tggtgccgtg gccgcagcaa agcacggcca
     2221 cgacgagcgt gtggcggttg aagccgtgat ccgcgctttc ttcgaagggc cggcgggcag
     2281 cggaaaaacg catcagctga tcgaggaagc ggtcacagca tcccgcgaag tgctcttgca
     2341 cccggagcaa aaactgctgg cgctcacgtt catgaacggc gcgcgtcacc ggttgaactt
     2401 tcggttcggc gagaaacgtg aactgcgcgg gcgattcgcc tgcctcacgt ttgactcttt
     2461 tgcgggaaca gtcgcgcgcc ggcgtcgtgc gctatcgcgg tcgctgccgc cggtgcgcca
     2521 cggccgagat accaacgact tcgaccgcac ctgcatcgaa gcggcgcggc tgctggagtt
     2581 gccggaggtc gcgaactggg ttgcgaattc atatccgctc gttgtcgtcg acgaggcgca
     2641 ggacctcgat gctcaccgtc tgcggctgct gaaggccctg agcgaggcgt gctgtgtggt
     2701 cgcggcggcg gacgagttcc agaacctcaa cgaccatgtc gatagcggca gcgttgtcac
     2761 gtggttgcgc atggcgaatc ggccacacgc gttgacgcgc atccgccgga cggcgcaaga
     2821 cggcctgctc agggcagccg gcgcccttcg cagcggcacc ggtatgtgtc acgagctggc
     2881 attcgaggat ggtttccagc cttgtcacgc cggcgatggc atccgcgtgg tttctcctcc
     2941 tgcgaaaaac tccggggccc tttcctgggc tgtggcgaac gagctgtcgg cgctgggcaa
     3001 ctatgctgtc atcctgacgc ctgacgcgaa ggggaagacc attcagcagg ttctggccag
     3061 tgttcagacg aaggctttta acaggaacaa aagggcagga acgaccttcg ggccttttcc
     3121 cctcgtgtgg gagcgccggg aggaagacga cgcggcggag tggctcgcgc ggttcgacgg
     3181 cgacggtact ctgaagttgc gcgacgttgt tgacacggtg tcgggcgcac agcggcccga
     3241 gtgccgctac atctgcgagc ggttggagag ggcgcggaat atccggggcg aaacgactgt
     3301 gacgcgcgag cgtttgacgc aggtgatcag agacgtcttg cgagacgcgg gtcacgcctt
     3361 gccgccgagc gggccaggac gacgcgtcat gacaattcca cgcgcgaaaa atcgcgagtt
     3421 cgggagtgtg ctcgtgctgt ggccgcattc tgtcgcgggc agtccggagc atcagcgacg
     3481 gctcctctac aatgccatca cgcgcgcgaa ggatcggtgc tccgtggtgg tgttcggcca
     3541 gggacgcact gacaaagcgc ccttctcata acgcgcggca ggccgcgctc aaacgaaaac
     3601 cgaaacggcg gcaggccgcc aaatacgacg gaagtggagt tcacggctcc tcaacttgcc
     3661 gatcatggac ccacattgga cccacgcggg agaattgcgc aggcgttgtt cccaccgtgt
     3721 tcccacgaag tccgagtaga caaagcgagg tggttgaaat aggccgattt tattggtcgg
     3781 ggcggccgga ttcgaaccgg cgaccccctg cgcccaaggc aggtgcgcta ccaggctgcg
     3841 ctacgccccg accgaggcct ccatcctatc aagctcccat gcggacgccg ggcctttcgc
     3901 gtcgcggcct tcctcaccac acgaagggca cgagcgcggc
//
LOCUS       DASZYN010000792         1765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1210284, whole genome shotgun sequence.
ACCESSION   DASZYN010000792 DASZYN010000000
VERSION     DASZYN010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210284"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VHE33_20030"
     CDS             <1..422
                     /locus_tag="VHE33_20030"
                     /inference="COORDINATES: protein motif:HMM:NF012903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HVT99798.1"
                     /translation="IGGNNLQINFNETATSSLTINGVNYNSAGLGLSSISGSAAGSFF
                     DTSTLSSLVTNINSAISNVQSQTETFGTNSSTISTRQSFTTNMINTLQTGASNLVAAD
                     QNQESANLLTEQTQQQLEISALSIANDANQSVLKLFG"
     gene            986..>1765
                     /locus_tag="VHE33_20035"
     CDS             986..>1765
                     /locus_tag="VHE33_20035"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99799.1"
                     /translation="MSDITLTSGIRQNLLSLQQTSRDLTTTQEALATGKAVNSAADNP
                     SAFFTSQNLTNSANSLSSLLDQIGQGLQTINAATNGLQGITSLLQQALSTVQQAQSAP
                     FSTAGVTTGAVDLSTAGAPSAAAGSLLLSVGTSTATITISSGEALSAIESAINSASTT
                     TLDGAVTATDNGSGDLVLSSTSTTFTIASNATSTGLGLTTGAVSGTSTTRTSLQSNYN
                     TLLTQINQLAADSGYNGVNLIGGNNLQINFNETATSSLTING"
BASE COUNT          391 a          605 c          470 g          299 t
ORIGIN      
        1 tgatcggcgg caacaatctg cagatcaact tcaacgagac agccaccagc tcgctgacga
       61 tcaacggcgt gaattataac tcggccgggc tcggcctgtc gtcaatcagc ggttcggcgg
      121 ccggctcttt cttcgatacc agcacgctct cgagcctggt caccaacatc aattcggcga
      181 tctcgaacgt gcagagccag accgagacgt tcggcaccaa ttcgtcgacc atctcgaccc
      241 ggcaatcgtt cacgaccaac atgatcaaca cgctgcagac cggcgccagc aacctggtgg
      301 cggctgacca gaaccaggaa agcgcgaacc tgctcaccga gcagacgcag cagcagctgg
      361 aaatctcggc actgtcgatc gctaacgacg ccaaccagtc agtgctcaaa ctgttcggct
      421 aaaatttctg gtataggcct gctggcatgc ggcggggcag aaatgccccg ccgttttgat
      481 tttgggcgta tccgacggac ttcggccggc gagcataccg cttcctcgcg acatcctccc
      541 tcaggcgacc cacttggcgg ccgacgacga aatgaaaaga cggcgcccga gccttcgcgc
      601 ggccatgcgg agcgagtgag cttgagcagg atggtcttct cagcgttttc gacggctggt
      661 tgacgcggcc gtccaatctc agcaagccca caacctgcaa tgtgacctga cccgtctgcg
      721 gctacccctc gaccgctggc ggcggggcta gtgcctcagt tttccaacta agacgttacc
      781 ggccgcaggc ccctcttgga cgggggccaa tccacacccg cggaccatgt cggactcgcc
      841 ttcgtcgaat acctctccgc acgaactgca acaatccatt aaccttatta cggtaaatat
      901 tcggtcaccg attttggatc gagccggtac ggcacgtcca gcaagtgcaa gccagtacgg
      961 cctgtaacca gaagggtata cgattatgtc tgatatcacc ttgacatcag gtattcggca
     1021 aaacctgctg tcactgcagc agacctctcg cgatttgacg acaacccagg aagcgctggc
     1081 aaccggcaag gcggtcaatt ccgccgccga caacccgagc gccttcttca cgtcgcaaaa
     1141 cctgaccaac agcgcgaact cgctgagctc gctgctcgac cagatcggcc agggcctgca
     1201 gacgatcaat gccgcgacca atggcctcca gggcatcacc agcctgttgc agcaggcgct
     1261 gtccaccgtg cagcaggcgc agtcggcacc gttttcgacg gcgggcgtca cgaccggagc
     1321 ggtggacctt tccaccgccg gcgctcccag cgccgccgcc ggcagtctgc tgctcagcgt
     1381 cgggacctcg accgccacga tcacaatcag ctcgggcgag gccctgagcg ccattgaatc
     1441 cgcaatcaac agcgccagca ccaccaccct cgacggcgct gtcaccgcaa ccgacaatgg
     1501 cagcggcgac ctggttctga gctccacctc gaccactttc acaattgcca gcaacgccac
     1561 ctcgacaggg ctcggcctca ccacaggagc agtctccggc accagcacaa cgcggacgtc
     1621 gctccagagc aactacaaca cactgctgac gcagatcaat cagctggccg cggactccgg
     1681 ctacaacggc gtcaatctga tcggcggcaa caatctgcag atcaacttca acgagacagc
     1741 caccagctcg ctgacgatca acggc
//
LOCUS       DASZYN010000793         2400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_504429, whole genome shotgun sequence.
ACCESSION   DASZYN010000793 DASZYN010000000
VERSION     DASZYN010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504429"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..597)
                     /locus_tag="VHE33_20040"
     CDS             complement(121..597)
                     /locus_tag="VHE33_20040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99800.1"
                     /translation="MVAILMLALLAGQAESADPYAPLRLYDGAWSLTMHEESGKSTTD
                     SLVNDCHLFGRYFACQQTVNGKVGALVVYVPGESPGHFYIQNVLPDDQAVGRGELTVE
                     GSRYTYAGHDGSSTHWWRTTNEFTGKDRIHFEQAASTDGKTWTTTVSGDEVRGTKP"
     gene            complement(730..1773)
                     /locus_tag="VHE33_20045"
     CDS             complement(730..1773)
                     /locus_tag="VHE33_20045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99801.1"
                     /translation="MKFVRTLSPLFMMLALATASAVAQTAIYSPANGSQVGSPFTLNM
                     KAATCSGQPVSAVGYSLDNSAQAAVFQVNYMNGPVSTSAGWHVLHVKVWNMYGGFCPT
                     DLSIDVVPGASATGSSNATSVIPSNAIGVGAIQTMGGWTMIHDGGTPGSSAGAMSVTG
                     SPAPDGKATYFQNVFNYYGGERYAVQFATDTAAQNFFYDTYVYIANDSNGISNLEFDL
                     AQTMQNGQTVMMGFQCDTWNNTWDYSVNGGSPTQFNDTWAHSSAYCNVHNWAPNAWHH
                     VQIWYTRNTSGWVTYHAVWLDGAQQAINVTAFSGYSLGWGPAVITQFQIDGASSGTSW
                     GNVYLDGMAVYRW"
BASE COUNT          481 a          771 c          627 g          521 t
ORIGIN      
        1 caaaaagaat gccgactcga atctttttca tgcgccggct gtcattctaa ataagacgcg
       61 gccttcgccg ctgtcgcgcc ggcaactcgc gattcgatcg cccatatcac atcgaagtcc
      121 tcagggcttc gtgccccgta cttcgtcccc ggaaaccgtc gtcgtccacg tctttccgtc
      181 cgtcgacgcc gcctgctcga aatgaatccg atccttgccg gtgaattcat tcgtcgtgcg
      241 ccaccaatgc gtgctgcttc catcgtggcc agcatacgta tagcgcgagc cctcgaccgt
      301 cagttccccg cggcccactg cctggtcgtc cggcaacaca ttttggatat agaagtgacc
      361 cggcgactcc ccgggcacgt acaccaccag cgccccgacc ttcccgttga ccgtctgctg
      421 gcacgcgaaa tatctgccga acaaatggca gtcgttgacg agcgaatccg tggtgctctt
      481 cccggactct tcatgcatcg tcaagctcca tgcgccgtcg tacaggcgaa gcggcgcgta
      541 cggatcggcc gattcggcct gtccggcgag taaggcaagc atcagaattg cgaccatgtt
      601 gatgggtctc cgtggcgttg tctttcctga cagtcaggag cgccatcgta tcactgctcc
      661 acagcaaaaa gaaaagcccc gagcgcaaac tcggggcttt cattttctct ttgctgatcg
      721 atcagcctct taccaccggt acactgccat tccgtccaga tacacattcc cccacgacgt
      781 tccgctgctg gccccgtcga tctggaactg ggtgatcact gccggtcccc accccaggga
      841 gtagcctgaa aaggcggtca cgttgatggc ctgctgcgca ccgtccagcc acactgcgtg
      901 ataggtcacc cacccggacg tgttgcgcgt ataccagatc tgcacatggt gccacgcgtt
      961 cggcgcccaa ttatgcacgt tgcaataagc gctcgagtgc gcccatgtgt cattgaactg
     1021 cgtcggcgat ccaccgttca ccgagtagtc ccacgtgttg ttccaggtat cgcactggaa
     1081 ccccatcatg accgtttgtc cgttctgcat cgtctgcgcc aggtcgaact ccagattcga
     1141 aatgccgttc gagtcattgg cgatgtaaac gtaggtgtcg tagaagaagt tctgagccgc
     1201 cgtatcggtt gcgaactgca ccgcatatcg ctcgccaccg taataattga acacgttctg
     1261 aaaatacgtc gcctttccat ccggcgcagg cgaaccggtc acgctcatgg ccccggccga
     1321 cgaacccggt gtccctccgt catggatcat cgtccacccg cccatcgtct ggatcgcccc
     1381 caccccaatc gcattggaag gaatcaccga cgtggcgttg ctgctgccgg tggcactcgc
     1441 gcctggaacc acgtcgatcg acaggtcggt cgggcagaat ccaccgtaca tgttccatac
     1501 cttcacatgc agaacgtgcc atcccgcact ggtggacacc ggtccgttca tgtaattcac
     1561 ctgaaagacc gctgcctgcg cgctgttatc gagcgagtaa cctacggcgc tcaccggttg
     1621 cccggagcag gtggccgcct tcatgttcag cgtgaacggc gagcccacct gggacccatt
     1681 cgccgggctg tagatcgccg tctgtgccac agccgacgca gttgccaatg ccagcatcat
     1741 aaagagaggg gagagcgttc tgacgaactt catcgagggt acctttgcga gggagatata
     1801 gcgccactgt gggcggtccg gccgagttcc tgcgcatgaa aaccgattgt cgcgtcctga
     1861 atcctgcaat cgaaactcat gcgatccgtt gtcctgccag ggtgcatcta cgctcttcca
     1921 gtcaatcccg ccgaatgccg cagcactcgt ttcaggctga tctggagccc ttccggagca
     1981 atcggattcc tcccggcaga gatcatggat ctcccgcgtg aagagtcgcc cgcatcgctc
     2041 aaaaagagca aaccggcttg cagcaaattg tcaggtggga ctgttctgag cttttaaccg
     2101 acgtcgaaca ctcgattcga gcagctcagc agacctctat atggatcggc ccgaacgaaa
     2161 ttcaaattcg ctccggaccg ggcgcctggc agcaaagtgc catggctggt gcgcattctc
     2221 cccgatcttc ctggatcatg gccccgggtt atcggttatg gttctgaccg caaggcaatc
     2281 agtcgcttcg agtgttaacc aatccttaca cacagtgatg gatcaggcaa cctttttctc
     2341 ggtggaaatt caccctgagc ctgcctcttt cgcgtcatgg cgcgccagtg atcaaaattc
//
LOCUS       DASZYN010000794         2131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1271871, whole genome shotgun sequence.
ACCESSION   DASZYN010000794 DASZYN010000000
VERSION     DASZYN010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271871"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            380..2023
                     /locus_tag="VHE33_20050"
     CDS             380..2023
                     /locus_tag="VHE33_20050"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HVT99802.1"
                     /translation="MSAKYIFVTGGVVSSLGKGLAAASIGCLLESRGLKVNLMKFDPY
                     LNVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTHAKLSRDNNWTTGRIYEQIILK
                     ERRGDYLGKTVQVIPHVTNEIKNAMRKVSAEGDVTIVEIGGTVGDIESLPFLEAIRQV
                     RQELGRENTVFVHVTLVPWIGAAQELKTKPTQHSVKELLSIGIQPDILLCRTDRHLSR
                     EVKSKIALFCNVEDQAVITARDVQSIYEAPLMFHSEGLDRLALKYLHIETPEPDLSKW
                     SALVERCYHPADEVSIAIAGKYVEYEDSYKSLKEALVHGALKYNLKLSVTWIEAEGLE
                     EPGYEEQLRGFDGILVPGGFGKRGIEGMLNAIRYAREQQVPYFGICLGMQTACIEYAR
                     NVCGLQGANSSEFDPATPHRVIYKLRELTGVEEMGGTMRLGAWTCLLEPGSAAAKAYG
                     KTEISERHRHRYEFNREYEALLTGAGLNITGTTPDGTYVEIVEIPGHPFFLGCQFHPE
                     FKSKPLEPHPLFARFIEASYANRLKRSQTQAEPMKVEVS"
BASE COUNT          460 a          716 c          577 g          378 t
ORIGIN      
        1 gccaaaggca acggtgaccg cagactctgc ctgtgcgccg gtgtggctcc ccggcctcat
       61 gccctgccga ggcgagggca tcgcgcacat cattctcgaa gtcactgacg acggctcacc
      121 caggctgacc tcctaccggc gtgtcgtcct ccacgtgctc ccaggcaact aacgcgagtc
      181 gattgacacc ggccgcgaaa caagttcgcc ttttactggc aactggcaac tggcaactgg
      241 caactggcaa ctggcaactg gcaactggca actggcaact ggctactaaa aactcttccc
      301 ttcgccctgg aatgctgcga tacttgatcg tgcccgcctg gttcgtccag cacacaaatc
      361 caaataaggt gacgcagaaa tgtccgcgaa gtacattttt gtgactggcg gagttgtgtc
      421 cagcctcggc aagggtcttg ccgccgcatc gatcgggtgt ctcctcgaaa gccgtggcct
      481 caaagtcaac ctgatgaagt tcgatccgta cctgaatgtc gatccaggga cgatgtcgcc
      541 cttccagcac ggcgaagtct tcgtcaccga cgacggcgcg gagaccgacc tcgacctcgg
      601 ccattacgag cgcttcaccc acgccaaact ctcccgcgac aacaactgga ccaccggccg
      661 aatctacgag cagatcatcc tcaaggaacg ccgcggcgac tacctcggca agaccgtcca
      721 ggtcatccct cacgtcacca acgaaatcaa gaacgcgatg cgcaaagtct cggccgaagg
      781 tgacgtcacc atcgtcgaaa tcggcggcac cgtcggcgac atcgaatcgc tgcctttcct
      841 cgaggccatt cgccaggttc gccaggaact gggccgcgaa aatactgttt tcgtccacgt
      901 cacgctggtg ccctggatcg gtgcagctca ggagctcaag accaagccca cgcagcactc
      961 cgtaaaagag ctgctctcga tcggaatcca gcccgacatt ttgctgtgcc gcaccgaccg
     1021 gcatctttcc cgcgaagtga aaagcaagat cgccctcttc tgcaacgtcg aggatcaggc
     1081 ggtcatcacg gcgcgcgacg tgcagtcgat ctacgaagcg ccgctcatgt tccactccga
     1141 aggcctggac cggctcgcgc tcaagtacct gcacatcgag acgcccgagc ccgatctgag
     1201 caagtggtcg gctctggtcg agcgctgcta tcaccccgcc gacgaagtct cgatcgcgat
     1261 tgccggaaaa tatgttgagt acgaggacag ttacaaatcc ctgaaagaag ccctcgtcca
     1321 cggcgcgctc aagtacaacc tcaagctcag cgtgacctgg atcgaagccg aaggactcga
     1381 agagccgggc tacgaagagc agctgcgggg cttcgacggc atcctcgttc ccggcggatt
     1441 cggcaagcgc ggcatcgaag gcatgctgaa tgcgattcgc tacgcccgcg aacagcaggt
     1501 cccgtatttc ggcatttgtc ttggaatgca aactgcctgc atcgagtacg cgcgcaatgt
     1561 ctgcggcctg cagggcgcga attcgagcga attcgatccc gccacgcccc accgcgtcat
     1621 ctacaagctg cgcgagctca ccggcgttga agaaatgggc ggcaccatgc gcctcggcgc
     1681 gtggacctgc ctgctcgagc ccggctccgc cgccgcgaaa gcctacggca aaaccgagat
     1741 ctcagaacgt caccgtcacc gttacgaatt caatcgcgaa tatgaagctc tgctcaccgg
     1801 cgcgggcctc aacatcaccg gcaccacgcc cgacggcacc tacgtcgaga tcgtcgaaat
     1861 tcccggccat ccgttcttcc tcggctgcca gttccacccg gaatttaagt ccaagcccct
     1921 cgagccgcat cccctgtttg cccgctttat cgaggccagc tacgccaacc ggctgaagcg
     1981 ctcccagacc caagccgagc cgatgaaagt ggaagtgagc tagcagctaa acagccgcgg
     2041 gcgcgtgcac cgcgatctcg acctttggcg gctgcaacag cgtgttgtga atcaggcaac
     2101 gatgcacggc ccgctcgact ccctcccggt t
//
LOCUS       DASZYN010000795         1884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_250621, whole genome shotgun sequence.
ACCESSION   DASZYN010000795 DASZYN010000000
VERSION     DASZYN010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250621"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            423..917
                     /locus_tag="VHE33_20055"
     CDS             423..917
                     /locus_tag="VHE33_20055"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HVT99803.1"
                     /translation="MSTQALIGDRKLTKYFRAPNQRIDGPLNIAMRVTIRSDANRIFQ
                     ALTQPEYLETWIAIPGAFADSHLIATQNLSSFRLDLYRNGVRDLTIEGEYKVRRRRKM
                     LFTWRTAGPTLNAESLVYIGLHGNFNETVLELHHRGISSPQEHAWQQEMWCESLNRLN
                     RLLS"
     gene            complement(952..1620)
                     /locus_tag="VHE33_20060"
     CDS             complement(952..1620)
                     /locus_tag="VHE33_20060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVT99804.1"
                     /translation="MEQIRILMVDDHGLFRESLSRLLDTVREVAVVGQCATVGEAFRE
                     LSQSNPDVILLDYDLGEESGLSLLAELKRRASSARVLMVTAGLHDEVVLRVMEMGASG
                     IFLKHGNPDRLMQAIQRVASGEIWLDDPALRSLLAERRTRTEHLERARPLTARQSEVL
                     RGILDGLANKEIAWKLNTSETAVKAAVQELFHKAGVRTRSQLVRIAIEQHSFDWLRPG
                     ADKS"
     gene            complement(1632..>1884)
                     /locus_tag="VHE33_20065"
     CDS             complement(1632..>1884)
                     /locus_tag="VHE33_20065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT99805.1"
                     /translation="SRDRRLFSVTAGTEGERVVVEFADSGGGVLHPEQLFRPFQEGAQ
                     SSGLGLYLSRAFLRSFGGEIRHLALSDGACFIVNLRSVA"
BASE COUNT          401 a          588 c          528 g          367 t
ORIGIN      
        1 gggaagtgcg aacggagtcg gacggggaaa ccgcagtcat tctcctcaac ggccagaagg
       61 tgccgatgag ccgaagctac cgctcgcgga tgcagcgtct gctgcatcgc tgatcgtcga
      121 tccacttttc tgtagaccgg atgattgcct cgctggccgg cagcagcgac aaaattcgcc
      181 ccatcgcgcg cgcaccccca tcggtcccag catcggacgt ttcatcccgg aattccaaat
      241 gcggaaacag cagggcggtt tctaccaggg agtgcacgaa gcttttgctc gctgcaatgg
      301 cgatgaagtt ggagcgccgg tattgatccc ggagatcccg catctcgtcc cagccttcgt
      361 gcttctatca caaaccattc aggtcgcgcc gcttcggttg ggtggttgct ctatggcatg
      421 gcatgagcac ccaggccctg attggtgatc ggaaactaac gaagtatttt cgcgccccca
      481 accagcggat cgacggtccc ctgaatatcg caatgcgagt gacgatccga tccgacgcga
      541 accgcatctt tcaggcgctc acccagcccg aatatctcga aacctggatc gcgattcctg
      601 gcgcctttgc agattcgcat ttgatcgcaa cgcaaaacct gagcagcttt cgcctcgatc
      661 tctaccgaaa cggcgtccga gatctgacga ttgagggcga atacaaagtc cgccgccgtc
      721 gcaagatgct cttcacgtgg aggacagctg gccctaccct aaatgcggaa agtctcgttt
      781 acattggcct tcatggaaat ttcaatgaga cggtgctgga acttcaccat cggggcatat
      841 cttcccctca ggaacacgcc tggcagcagg aaatgtggtg cgaatcgcta aaccggctga
      901 accggctcct gagctgagaa tcagccagga gcagttgtga acctcgtcgc gtcagctctt
      961 gtctgcacca gggcgcagcc agtcgaagga atgctgctca atagcgatcc tgaccagttg
     1021 gctgcgggtt ctgactcccg ctttgtgaaa cagttcctga accgcggctt tgaccgccgt
     1081 ttccgaagta ttcagtttcc aggcaatctc cttgttggca aggccgtcga ggataccgcg
     1141 cagcacttcg ctctgccgcg ccgtaagcgg ccgggcgcgc tccagatgct ctgtgcgggt
     1201 tcgcctttcg gccagcaggc tgcgcagcgc gggatcatcg agccagattt cgccggatgc
     1261 gacacgctga atggcctgca tcaaccgatc agggttcccg tgcttcagaa aaatgcccga
     1321 cgcccccatc tccatgacac gcaacaccac ttcatcatgc agaccggccg tgaccatcag
     1381 gacacgcgcg ctgctggccc ggcgtttcag ttcggcgagc agactgaggc ccgactcctc
     1441 gccaaggtca tagtccagga ggatgacgtc gggattcgac tgcgaaagct ctcggaacgc
     1501 ttctcctacg gtggcgcatt gtcccacgac cgccacctcc cgaactgtgt cgaggagacg
     1561 gctcaggctc tcgcggaaga gcccgtgatc atcgaccatg aggatccgaa tttgctccat
     1621 cgggcgctgt cctaggccac actcctcagg ttaacgataa agcatgcgcc gtcagacaag
     1681 gcgagatgac gaatttcgcc gccgaaggaa cgcaggaatg ctcgcgagag gtaaaggccg
     1741 agcccggacg attgcgcgcc ctcctgaaaa ggacgaaaca actgctctgg atgcagaacc
     1801 ccaccgccgc tgtcggcaaa ctccaccacc acacgttcgc cttccgttcc ggcggtaacg
     1861 gaaaagaggc gccggtcccg tgag
//
LOCUS       DASZYN010000796         1770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_114132, whole genome shotgun sequence.
ACCESSION   DASZYN010000796 DASZYN010000000
VERSION     DASZYN010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114132"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..>1770)
                     /locus_tag="VHE33_20070"
     CDS             complement(186..>1770)
                     /locus_tag="VHE33_20070"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HVT99806.1"
                     /translation="FGLFTGTGSFSTTGGLVLAVVSLVMGGVIYAFAYALRPAQVAVA
                     SGPALAGASGGIFTGGEPLSDEGRLTARDFSRIFEQTWLPFFQWSRVDAVYLAMGRGL
                     LTCARICNVALAWLEERASVSMVVLSALVLAALRWFNMPVPEVRAVEPMHAAPLLTAA
                     CATAAVGLLGVALSQRATRRLALWMSLSSAAAAAGLVVHGEWLRFALVESAAILTIPL
                     VWRPQVSRGAKNAYVIMVAVSLALLTASQILIHDPAAAQAFLLTGACLKLAAIPFLFW
                     VARVADELPAVVLGQIVAVVDIAAFADLVVITQQHPLLLGSPAVWLTVAGVTAFAAAI
                     LMLAQRSLKRLLVLSSVEDVGFLFLGLVSASAVGVSGAVIGATAHALAKALLFATLAT
                     PESDGVLRADRTGIASRYPMSAFGFLFGMLAMLGIPPMVGFVGRWRLYEAALRIHPAL
                     LAVFIASSGFALIAYVIALTRAWWGPPAAGANEADCGDSQPHQEPFLVRAVVLVLVAL
                     LLVPGLWPGSLRFLMGGLP"
BASE COUNT          421 a          574 c          545 g          230 t
ORIGIN      
        1 cgttacacaa aggatatcgg cgtggcgtgg gctgccctgc agccgcaccc cgagctgctc
       61 cgcgtcataa cgaggcgtca aactcgcgac cagttcaata tcgcatccgt tacagctgcc
      121 ggaattcata tgaaacagcc acggactgcg gcggcggcag gagcttatga cacgagaggt
      181 gagagtcatg gaaggcctcc catcaggaat cggagcgatc cgggccataa gccgggcaca
      241 agcaggagcg ctaccaggac caacaccaca gcccgcacga gaaacggttc ctgatgtggc
      301 tgggagtccc cgcaatcggc ctcattggct ccggcagcag gcggtcccca ccaggcccgc
      361 gtcagtgcta tgacgtaggc gatcagggca aatccggaag aggcgatgaa tacagcaaga
      421 agcgcagggt ggatgcgcag cgctgcctcg tagaggcgcc agcggcccac gaatccaacc
      481 attggcggaa tgccgagcat ggccagcatc ccaaacagaa aaccaaaggc actcatcgga
      541 tagcggctgg cgattccggt gcgatctgcg cgcaaaactc catcggattc gggcgttgcg
      601 agtgtggcga agagcagtgc cttggcgagg gcgtgggcgg tggccccaat aacagcaccc
      661 gaaacgccga ccgctgacgc cgacacgaga cccaaaaaaa gaaatcccac atcttccacg
      721 ctcgacagca ccagcagccg tttaaggctc cgctgagcca gcatcaggat cgcggctgcg
      781 aacgcagtga cgccggcaac cgtgagccaa acagcgggag aaccgagaag aagcggatgt
      841 tgctgtgtga tgaccactaa atcggcaaat gcggcgatgt cgacgacggc cacgatttgt
      901 ccgagcacaa cggcgggcag ttcatccgcc acccgcgcaa cccagaacag aaagggaatc
      961 gccgccagct tgaggcaagc ccccgttagc aggaacgcct gcgctgctgc cggatcgtgg
     1021 atcagaatct gactcgcagt cagcagagcc agggaaaccg ccaccatgat gacgtacgcg
     1081 ttcttcgctc cacgggagac ctgaggccgc cagaccagcg gaattgtgag gatggcggca
     1141 gattccacaa gtgcaaagcg cagccattct ccatgcacga cgaggcctgc ggccgcagct
     1201 gcactgctca gcgacatcca cagggccagc cgtcgagtcg cccgttggct gagcgcgacc
     1261 ccgagcaggc ccaccgcggc cgtcgcacag gctgcagtca gcagcggcgc cgcatgcatc
     1321 ggctcgacgg cgcgcacctc cggcacgggc atgttgaacc agcgaagcgc agccagcaca
     1381 agcgcagaca gcacaaccat cgagacagaa gcgcgttctt ccagccaggc cagtgcgaca
     1441 ttgcaaatgc gtgcgcacgt cagcagacct cgccccatcg ccaggtacac cgcatcgact
     1501 cgcgaccatt ggaagaaggg gagccacgtc tgctcaaaaa tgcgggaaaa gtcgcgggcg
     1561 gtcagccgtc cttcatcgct gagcggctcg ccgcccgtaa agatgccccc cgatgcgcct
     1621 gcgagcgcag ggccggaggc cacagcaacc tgcgcaggcc gcagggcgta ggcgaatgcg
     1681 tagatcacgc cgcccatcac cagcgaaacg accgcgagca ccagaccgcc cgtcgtcgag
     1741 aagctgccgg taccggtgaa caggccaaac
//
LOCUS       DASZYN010000797         3258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1211166, whole genome shotgun sequence.
ACCESSION   DASZYN010000797 DASZYN010000000
VERSION     DASZYN010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211166"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..255
                     /locus_tag="VHE33_20075"
     CDS             25..255
                     /locus_tag="VHE33_20075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99807.1"
                     /translation="MFLSVVILGRFTTETHAMALYISTDTPPLFTPRPGTFPYGLFAE
                     AKLHTCKRRAEYYVVSLRVVVATAGRRIATPY"
     gene            complement(272..595)
                     /locus_tag="VHE33_20080"
     CDS             complement(272..595)
                     /locus_tag="VHE33_20080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99808.1"
                     /translation="MVPSYALAYVVLLWTIAFTAKALDGGAGNVDERSGSAQLPYHDL
                     KRHFALARRWELDTTESSTPTDIAATLRNAKERLDLARSNNMVLGSLQPADYLNRWSA
                     VLTNP"
     gene            complement(651..794)
                     /locus_tag="VHE33_20085"
     CDS             complement(651..794)
                     /locus_tag="VHE33_20085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99809.1"
                     /translation="MAGFIASAIVVVAVGIWYFTQRRSSKVTPSGNTPTSPSWRGWLY
                     RDS"
     gene            complement(859..2325)
                     /locus_tag="VHE33_20090"
     CDS             complement(859..2325)
                     /locus_tag="VHE33_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HVT99810.1"
                     /translation="MQVVWSSRRGSRRIEHLGSAHDEAGVAALKAAAAQRLAAGQAQL
                     DLGLAERSAAEPLPITSSKSALLWEALCAAYDKVGFANVADGDEAFRQLVLARIIEPT
                     SKADSLRVVEETGVVPVSYPTLNRRLPVFAKPAFRQALSTACAAHAQLGPASLVLYDV
                     STLHFETDAGDGFREPGFSKERRLDPQITLGLLTDAGGFPLTVAAFEGNKAETATMLP
                     VINAFKAAHQLTDVTVVADAGMISEANQVALHAAGLSYILGARIPFLPDVVREWRDKH
                     SDEAIPDGLVLTQPWPSTSGEKTRGIPDRIIHYQYRHDRARRTLRGIDEQVAKAERAV
                     DGHAPVKRNRYIQLTGATKSVNRTLEAKARALAGWKGYTTNLVSQPATFVIEAYHQLW
                     RIEKAFRMSKHDLQARPIYHRTRDSIEAHLSVVFAAMAVSHWIEHQTGWSIKKFVRTA
                     RRYRTVTIQAGKHTLTAAEPPPPDLAEILANIHSLRAH"
     gene            complement(2571..2981)
                     /locus_tag="VHE33_20095"
     CDS             complement(2571..2981)
                     /locus_tag="VHE33_20095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003899157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-dependent biliverdin reductase"
                     /protein_id="HVT99811.1"
                     /translation="MANTTTRLTDDALAFLSERHLAMLTTLRADNSPHVVAVGFTFDP
                     KTHIARVITTGGSQKAINADRGGLAVLSQVDGARWLSLEGRSKVNSDVDAVRDAELRY
                     AQRYRTPRPNPRRVVIEVQIERVLGSSDLLDRGA"
     gene            3017..>3258
                     /locus_tag="VHE33_20100"
     CDS             3017..>3258
                     /locus_tag="VHE33_20100"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HVT99812.1"
                     /translation="MDDTGAAPVLILGGRSEIGVELARRLAPGTTVVLAARRADQLDE
                     QVAALKAAGAKTVHTMEFDADDLDSHGPLVAAVVAD"
BASE COUNT          607 a         1056 c         1011 g          584 t
ORIGIN      
        1 gcagactgat ccagggccat cgcgatgttc ctctcggtag tgatccttgg tcgtttcacc
       61 acagagactc acgcgatggc cctctacatc agcaccgaca cgccgccact atttacacca
      121 cgccccggga cgttccccta cggtttgttt gccgaggcca agctgcacac ctgcaagcgg
      181 cgagctgaat actacgtcgt gtcactgcgg gtcgtcgttg ccactgcggg tcgaaggatt
      241 gcgacgccgt attgagtacc gcacccggcc ttcaaggatt tgtaaggacg gcgctccaac
      301 ggttgagata atcggctggc tgcagcgatc ccaggaccat gttgttgctt cgcgctaagt
      361 cgagtcgttc cttcgcgttg cgcagggtcg ctgctatgtc ggtcggagtc gagctctccg
      421 ttgtatcaag ctcccatcgc cgcgcaagcg cgaagtgcct cttgagatcg tggtagggca
      481 gctgcgcgga accgctgcgc tcatcgacat tgccggcacc accgtcaagc gctttcgcgg
      541 tgaacgcaat cgtccacagg aggacgacgt aagcgagcgc gtagctcggc accatgaccc
      601 acaatgattt tcccccgtga aacgcccagc tacgccgctc acggtgtcac ctacgaatcc
      661 cgatagagcc acccccgcca tgatggcgac gtaggcgtat tcccgctcgg cgtgaccttg
      721 ctggatcttc gttgagtgaa gtaccagatc ccaacggcga caacgacgat cgccgaggcg
      781 atgaatcccg ccacgatcca caccggcagg ggcttgctgg gggctatgtg ggctgacttg
      841 aacccgactt ttatcaggct agtgcgcacg taggctgtgg atgtttgcga ggatttcggc
      901 gaggtcgggt ggtggcggtt cagcagcggt cagggtgtgc ttgccggctt ggatagtgac
      961 ggtacggtag cggcgtgcgg tgcgaacgaa tttcttaatg ctccaaccgg tttggtgctc
     1021 gatccagtgc gataccgcca tcgcggcgaa gacaacgctg aggtgggctt cgatggagtc
     1081 gcgggtgcgg tgatagatcg ggcgggcttg caggtcgtgc ttggacatcc ggaacgcctt
     1141 ttcaatgcgc cacaactggt gatacgcctc gatgacgaaa gtggccggtt ggctcaccag
     1201 gttggtggtg tatcccttcc acccggccaa cgcccgggcc ttggcctcca gtgtgcgatt
     1261 cacgctcttg gtagccccgg tgagctggat gtagcggtta cgtttgaccg gggcatgccc
     1321 gtcaacagca cgttcggcct tggccacctg ctcatcgatc ccccgcagcg tgcgccgggc
     1381 ccggtcatgg cggtactggt agtggatgat ccggtccggg atgccgcggg ttttctcgcc
     1441 gctggttgat ggccagggct gggtgagcac caggccgtcg gggatcgcct catcggagtg
     1501 cttgtcgcgc cactcgcgca ccacatcagg taaaaacgga atcctcgcgc cgagaatgta
     1561 ggacagccct gccgcgtgca gggcgacctg gttggcttcc gagatcatcc cggcatcagc
     1621 gacgaccgtg acgtcggtga gttggtgagc ggccttgaac gcgttgatca ccggcagcat
     1681 ggtcgcggtc tctgccttat tgccctcaaa ggccgccacc gtcaacggga acccgccagc
     1741 atcggtcaac aaccccaggg tgatctgcgg gtccaaccgt ctttccttgg agaatccggg
     1801 ctcccggaac ccatcaccag catcggtctc gaagtgcagc gtcgacacgt catagagcac
     1861 cagactggcc ggacccaatt gcgcgtgggc agcgcatgcg gtcgaaagcg cttgccggaa
     1921 agcgggtttg gcgaacaccg gcagacggcg gttcagcgtc ggatacgaca ccgggacgac
     1981 cccagtctcc tcgacgaccc gaagtgaatc ggccttgctg gtcggttcga tgatccgggc
     2041 caacaccaac tgacgaaacg cctcatcccc atcggcgacg ttggcgaacc cgaccttgtc
     2101 ataagcagcg cacaatgctt cccacagcag cgccgacttc gaggacgtga tcggcaacgg
     2161 ctcggccgcc gaacgttcgg ccaagccaag gtcgagttgc gcttggccgg cggccagtcg
     2221 ctgcgccgcg gcagccttca aagcggccac cccggcctcg tcatgcgccg aacccagatg
     2281 ctcgatccgg cgtgacccgc gccgcgagga ccacacgacc tgcacagcca tcgcccccga
     2341 ggatgtcttc accgtgcgca cataggccac gcaggcaccc tacggcccac cggctagtgc
     2401 acaaatccgc accaggccac aaccgaagac acaggtcaca gccctacaca accgccggat
     2461 cgccgacagt gataaaagtc aggtcttacg acgagctcga tgcatcactt ctcgaaggat
     2521 tctcggacaa cgtcgtttca cgatttaagt cggaaatgaa ttgacgacga ctacgcgccc
     2581 cggtccaata gatccgacga cccgagcacc cgctcgattt gcacttcgat gaccacccgt
     2641 cgcgggttgg gccgcggcgt gcggtagcgc tgggcgtaac gcaactcggc gtcgcgcacc
     2701 gcgtcgacat cgctgttcac cttggaccgg ccctccagcg acaaccaccg cgccccgtcg
     2761 acctggctga gcacggccag cccgccgcga tcggcgttga tcgccttctg cgacccgccg
     2821 gtggtgatca cccgggcaat gtgcgtcttg gggtcgaagg tgaaacccac cgccaccacg
     2881 tgcggcgaat tgtcggcccg cagcgtggtg agcatggcca gatggcgctc cgacagaaac
     2941 gccaacgcgt cgtcggtgag ccgggtagtg gtattcgcca tcaccgccca cgctagcgca
     3001 ggtaataatc gcagccgtgg acgacacggg cgcagctccg gtactaatcc tgggcggccg
     3061 cagcgaaatc ggcgtcgagc tggcccgacg gctggcgccg ggaacgacgg tcgtgctggc
     3121 ggcgcgccgg gccgaccaac tcgacgagca ggtggccgca ctgaaagccg ccggggccaa
     3181 aacggtgcac accatggagt tcgacgccga cgacctggat tcccacggcc cactggtcgc
     3241 cgcggtcgtc gccgatca
//
LOCUS       DASZYN010000798         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1272758, whole genome shotgun sequence.
ACCESSION   DASZYN010000798 DASZYN010000000
VERSION     DASZYN010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272758"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..1098
                     /locus_tag="VHE33_20105"
     CDS             235..1098
                     /locus_tag="VHE33_20105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99813.1"
                     /translation="MTQELVQLTADNPLRGLQRADGTWSARDVQKSGYVHATWRSWEL
                     ALQRGQALLEGQYGNGAGQAHIASASKKVGIGLNGQREVNDYTLTALGLFAACEQSQL
                     PDLDIWFVHAGLEAAGLSKEVDVMPDRTPALPPHLELPSADRIINLGLESVTVYPDGR
                     QELKFNPALAAAERRLREEKSERRRQAREDRDRRLRNRVMLPSGNLEEFFNMGKGTWR
                     HLLYAVNRYVGVRAPFDEVEKWVRSYQAQLQPDIREVNITIEDDIEYPSYKLVLDARE
                     YVLIKLGLPLQ"
     gene            1411..>1648
                     /locus_tag="VHE33_20110"
     CDS             1411..>1648
                     /locus_tag="VHE33_20110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99814.1"
                     /translation="MNDKIRPEAFAQIARDFLALHGDDWDTLHVFASLFPDGDGGLRA
                     PVVSAIDPAFPPEMYPLIMLSQVKAAIGHQKAVPA"
BASE COUNT          355 a          537 c          498 g          258 t
ORIGIN      
        1 cccccaccag gtgtcgtgtc gggaaaagct cgccgccccg gctcaggcac gcgcgctgcc
       61 gcagggcact gagcgatggt aggcccccct gcggccccag ggacagagcg acacggtaac
      121 agtctcaggt gactctccgc atacgaatca cggagagtat tcattactct cagttaccct
      181 ccgtttagag cgtaacattc tgtaggtgta aacagttgag gtataatgcc acacatgacg
      241 caagaactag ttcaactaac cgcagacaac ccgctgaggg gtctgcaacg agctgatggc
      301 acctggtccg cccgtgacgt acagaagtcc ggctatgttc acgcaacctg gcgcagctgg
      361 gagctggccc ttcagcgcgg gcaagctttg cttgaagggc agtacgggaa cggcgcaggt
      421 caggcccata ttgcctccgc cagcaaaaag gtgggaatcg ggctaaacgg acagcgcgag
      481 gtgaacgact acaccctgac tgcgcttggc ctgttcgccg catgcgagca gtcgcaactg
      541 ccggatttgg acatatggtt cgtgcacgcc ggccttgaag ccgcaggctt gagtaaagag
      601 gtcgacgtta tgcctgaccg cacacctgcc ctgccgccgc atctggagct gccgagcgcc
      661 gaccggatca tcaacctcgg actggaaagt gtgacggtct accctgacgg ccgccaggag
      721 ctgaagttca acccggcgct ggctgctgct gaacgccgtc tccgcgagga gaagagcgag
      781 cgccgccgcc aggcccggga agaccgcgac cgccgtctcc gcaaccgggt gatgctgcca
      841 tcgggaaacc tggaggaatt tttcaacatg ggtaagggca cctggcgcca cctgctttat
      901 gccgttaatc gctacgttgg ggtccgtgca ccctttgacg aggtcgagaa gtgggtcagg
      961 agctaccagg ctcagcttca gccggacatc cgggaggtga acatcacgat cgaagatgac
     1021 atcgagtacc cgtcctacaa gctggtactt gacgcccggg agtacgtcct gatcaagctc
     1081 gggctcccgt tgcagtaact gcaaaggaga gagtatgacg gccagtagtt tggcggaacc
     1141 aactccgcac acgccgtcca ccaagggatg caaattaaca gtgacaccgg gccataacga
     1201 tcacgcaacc cacctaggcg gcgcccggcc acgcgacaca cgaaccccac gagtccagcg
     1261 ccgggccaag cagcatacgg agcccaactg cgacacgccc ggccaggcac accccggact
     1321 ccagtccgta aacgccgggc caagaccttt acggaaacca cactcggcac gcccggctcc
     1381 cgggacaccg tcacgaaagg aatcacccgc atgaatgaca agatccgccc ggaggcgttc
     1441 gcccagatcg cccgggactt cctcgccctg cacggggacg actgggacac cctgcacgtc
     1501 ttcgcctcgc tgttccccga cggggacggc ggcctcaggg cgccggtggt cagcgcgatc
     1561 gacccggcct tcccgccgga gatgtacccg ctgatcatgc tgagccaggt caaggcggcg
     1621 atcgggcacc agaaggcggt cccggccg
//
LOCUS       DASZYN010000799         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1024229, whole genome shotgun sequence.
ACCESSION   DASZYN010000799 DASZYN010000000
VERSION     DASZYN010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024229"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VHE33_20115"
     CDS             complement(<1..692)
                     /locus_tag="VHE33_20115"
                     /inference="COORDINATES: protein motif:HMM:NF015970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HVT99815.1"
                     /translation="MTEKRRLLLFAAGAIGAAAIYCAGIRNMPACGEYRGPYGNVIAS
                     LAVYERHATDTVNAITYDYRGFDTLGEEFILFSAVTGVMLLLRPEEGKKQSAGANPAS
                     FRDRAALSETVQVFTTAMFGFSVLFGIYIGTHGNLTPGGGFQGGVIVAAAPMLLYIAE
                     NTDSFQRILSHPLTEFFEAAGAGAYALVGCIALLVGAPFLTNILPLGQTGDVFSSGTI
                     AVISTCVGFEVAA"
     gene            complement(685..954)
                     /locus_tag="VHE33_20120"
     CDS             complement(685..954)
                     /locus_tag="VHE33_20120"
                     /inference="COORDINATES: protein motif:HMM:NF024641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HVT99816.1"
                     /translation="MTILGVGLLALIAISAFVIVRTDDVTHQLLGLGFYGLLFGLLFF
                     VFQAPDVALSQITVGAVALPLLIMLAITRMKHSKALADRGKPHHD"
     gene            complement(951..1298)
                     /locus_tag="VHE33_20125"
     CDS             complement(951..1298)
                     /locus_tag="VHE33_20125"
                     /inference="COORDINATES: protein motif:HMM:NF015302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HVT99817.1"
                     /translation="MTAHRFVVDALLGIVAIACWVGVLGMIRMRDPYQALHYLGVPAM
                     AGMAALTAAVWIDTGWSQATWKAGIILVVLAAANSVGTHAAARAFRARRKDHWEALPD
                     DPEVEFLGGRPRA"
     gene            complement(1295..1546)
                     /locus_tag="VHE33_20130"
     CDS             complement(1295..1546)
                     /locus_tag="VHE33_20130"
                     /inference="COORDINATES: protein motif:HMM:NF015993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MrpF/PhaF family protein"
                     /protein_id="HVT99818.1"
                     /translation="MNRWILTALATSGCLVPCAWACLRGSAERRLVGLEMTGIIVTLL
                     MVLLSVGEGRLIFFDLPLTLSVLAFGAGLVFARFLEKHL"
     gene            complement(1548..2042)
                     /locus_tag="VHE33_20135"
     CDS             complement(1548..2042)
                     /locus_tag="VHE33_20135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99819.1"
                     /translation="MSQRLRSAVQASICVVTLAVFWVACVSTVRPHEMLVGAAAVAFS
                     AIFSISVIRTLPLHFRPSPKMLAEAWRIPGYVVLDCIVVTMVLARDLMGLRASSLFRS
                     APWHRVTDSGHDTAARTLAVACTTASPNMIILGMDCERGQMLFHQLRKAGVPAMTRHL
                     GAEG"
BASE COUNT          485 a          643 c          725 g          351 t
ORIGIN      
        1 gccgcaacct cgaagccgac acaggtgctg atgacggcga tggtgccgga ggagaacaca
       61 tccccggttt gaccgagcgg cagtatgttg gtgaggaaag gcgcgccgac aaggagtgcg
      121 atgcaaccga caagggcgta agctcctgcc ccggccgcct cgaagaattc ggtgagagga
      181 tgggaaagaa tccgctggaa cgaatcggta ttctcggcaa tgtagagcag catgggggcg
      241 gcggcgacga tcacaccacc ctggaatccg ccgcccggag tgaggtttcc gtgggtgccg
      301 atgtagatgc cgaagagaac cgagaagccg aacatggcgg tagtgaagac ctgcacggtt
      361 tcgctgagag cggcgcgatc ccggaaggat gccggattgg cgccggcaga ctgtttctta
      421 ccttcctcgg ggcgcagcag aagcatcacc cccgtcacgg cgctgaagag gatgaactct
      481 tcacccagcg tgtcgaatcc acgatagtcg tacgtgatgg cgttcacggt atcggtcgcg
      541 tggcgttcgt agacggcaag actggcgatc acgttgccat aggggccccg atactctccg
      601 caggccggca tattgcggat tccggcgcag tagattgcgg ctgcgccgat agcgccggcg
      661 gcgaagagca acaggcgtcg cttctcagtc atggtgcggc ttcccgcgat cggcgagtgc
      721 tttgctgtgt ttcatccgcg tgatcgccag catgatcaga aggggaagcg caacggcgcc
      781 gacggtgatt tgcgagagag caacgtccgg agcctgaaag acgaagaaga ggagcccgaa
      841 gagtagcccg tagaagccaa ggcccagcag ttgatgggtc acgtcatcgg tacggacgat
      901 cacgaatgcg ctgattgcga tgagagcgag gagacccact ccgagaatcg tcatgcgcgc
      961 ggcctcccac ccaggaattc gacctcgggg tcatccggca aggcctccca gtgatctttc
     1021 cggcgcgcgc gaaaggcgcg agcggcggcg tgcgttccta ccgaatttgc ggctgcgagg
     1081 acaaccagga tgatgccggc tttccatgtg gcctgactcc agccagtgtc aatccacacg
     1141 gcagcggtga gagcagccat gcctgccatc gcaggaacgc cgagatagtg cagcgcctgg
     1201 taaggatcgc gcatgcggat catacccagc acacccaccc agcacgcgat ggcaacgatg
     1261 cccagcagtg cgtccacaac gaaacgatgt gctgtcacag atgcttctcc agaaaacggg
     1321 caaacacgag gcctgcccca aaggccagca cagaaagggt cagcggcagg tcaaagaaga
     1381 taaggcgtcc ttcgccgacg ctcagcagga ccatcagcag agtgacgatg atgccggtca
     1441 tctcgaggcc gacgagtcgc cgctccgccg agccgcgcaa acaggcccag gcgcagggaa
     1501 ccaggcagcc actggtagca agagcggtta gtatccagcg attcatgtca gccctcagcg
     1561 cccaggtgcc gcgtcatggc tggaacgccg gcttttcgca attgatgaaa gagcatctga
     1621 ccccgctcgc agtccatgcc gaggatgatc atgttgggcg aagcagtggt gcaggcgacg
     1681 gcaagcgtgc gcgcggcagt gtcatggccg gaatcggtaa ccctatgcca gggcgcggaa
     1741 cgaaacagcg atgaagcgcg gagccccatc aaatcgcgcg ccaggaccat cgtgacgacg
     1801 atgcagtcga ggacgacgta tccaggaatg cgccaggcct ctgccagcat tttcggtgac
     1861 ggtctgaaat gcaggggcag ggtgcggatc accgatatcg aaaagatcgc cgagaaggcg
     1921 acagccgcag cgccgacgag catctcgtgc gggcgaacgg tcgacacgca cgcgacccaa
     1981 aagacagcga gcgtgacaac gcagatcgat gcctggactg cactgcggag cctctggctc
     2041 atgcgagttt ggaggggctg cggcctggtg aggatgcctg gacgcggttt tggtggtttg
     2101 ccttcaaagc catccgcgct gactttttgg tcgcgcggtg tgttgggtca ggccgcctgc
     2161 cgctgccgcc tctccagata tccgcccacg gcggccagaa tcac
//
LOCUS       DASZYN010000800        10653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_963551, whole genome shotgun sequence.
ACCESSION   DASZYN010000800 DASZYN010000000
VERSION     DASZYN010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 10653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10653
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963551"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VHE33_20140"
     CDS             complement(<1..634)
                     /locus_tag="VHE33_20140"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HVT99820.1"
                     /translation="MTSKKELLNQPIRHIDIKQHNVVALVDAMQSMAYSSRDLARAAS
                     IYERMLRDRDCGVILCLAGSLISAGLKQIFVDLIRNNMVDAIVSTGANIVDQDFFEAL
                     GFQHYIADDLYTSGTQDGELRELMIDRIYDTLIDEEELRACDETTEKVADSLAPRPYS
                     SREFIRAMGAWLEANGKTPQMGGVDSIVLSAYQKDVPIFCPAFSDCSAGFG"
     gene            complement(741..1481)
                     /locus_tag="VHE33_20145"
     CDS             complement(741..1481)
                     /locus_tag="VHE33_20145"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HVT99821.1"
                     /translation="MGPKRFCTLCAERYTLSLMIDGRRAVRYDAALACSTLAAADPKL
                     GDLIRRVPFTLKLKSQHSPFEALLESIIYQQLHGKAAAAILKRLLTAFGELHPSPEHL
                     LQAPDDMLRACGLSASKTKALRDLAAKTIDGTVPTLTAIRRMPDDEIIERLTEVRGIG
                     TWTVEMLLIFRLGRPDVLPVTDYGVRKGFALTFLRLPKSKPFDASMLATADQITRRAE
                     KWRPWRSVASWYMWRACDLAGKTMVLPD"
     gene            1987..2874
                     /locus_tag="VHE33_20150"
     CDS             1987..2874
                     /locus_tag="VHE33_20150"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HVT99822.1"
                     /translation="MPQIFDRSSNALARMSLILTGLIVISLGLTLDEVQRSPWVTRQG
                     QRADQPVPFSHKHHVEGLGLQCQYCHTTVEKSSYAGIPPTKTCMNCHAQIWTNAQLLE
                     PVRQSWATGASIPWTRVHDLPDYVFFNHEIHVNKGIGCSSCHGRVDEMPIMYMQNSLQ
                     MEWCLNCHRNPEKNLRPTSEIYNMAWQGASELRPVWCAAEDVPAGSPTAGGVTCTEEN
                     PKMNGGVQMASLSRPPTGLHAQAGGTAIGSEDTNPIQLPAGVHYTEFTSQQALGQFLE
                     KHYDIRNPRELTSCEVCHR"
     gene            2958..6029
                     /locus_tag="VHE33_20155"
     CDS             2958..6029
                     /locus_tag="VHE33_20155"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HVT99823.1"
                     /translation="MQIEPKKLTLAEIRAKLANQKGKKFWRSVDELADSSEFAELVNK
                     EFPSQASEWIDPVSRRGFLKVMGASMALAGLAGCTKQPDEPIYPYVTAPPDLTLGKPV
                     YFASAFPFTTGAVPVLIKSAAYRPIKVDGNPEHPYVRGGSDPITQATLLDMYDPDRSQ
                     HILYRGETRNWASFLAAFRSMLADKKPAGGQGLYFLSATVTSPTLAAQWQQAQKNYPH
                     AKFVQYDPANRDSHYRASQQAFGQFVDAQYRLDQADVIVSLDADFLGGAQFPGFHKLA
                     RDYAQKRKLDGTDQMNRLYVIETVTTTTGFKADHRLAVKPSQIAAFAQALAGAVGGGG
                     SSAGNWSDHDAKYLQGVAADLKANGGKCVVIPGEFAPPEVHLAAFAINQALGNVGKTV
                     VYTDPVNPMPSIQCDDLKALVADMNAGKVEWLVILNANPLYSAPADLDFESAFNKVNT
                     VVHLGSHWDETGQQAHWHINNAHYLEMWSDARAYDGTVSIVQPMIDPMYGGHSAHEIL
                     QSMLDNPDVSAYDAVRATWLGNGGLLSKGDAEFNWRKALHDGWIDGTAFAPKTVAPKG
                     GLATSAGTAAASGVEVVFRPDAHVYDGRYANLGWMQEIPRPVTNLSWDNAAQMSFATM
                     EKNGLAENDAVEISVGGKKIIGGALAVPGLPDDVVVVTLGQGRRIGRVAGGVGYNGYL
                     IQQSGAPWMLAGASLRKTGDIYDICVTKSHHIDQRSVAHGGDGSGTHSIEGNESLDRG
                     IIRYTTLTEYKKDPEFAHEGVARWTPEPNESMFPAYRYDKNAWGMSIDLNSCIGCNAC
                     VTACFAENNIPVVGREQVKIGRLMQWIRIDTYFEGDLAAPRAHFQPMTCHQCENAPCE
                     QVCPVGATVHSPEGLNLMVYNRCVGTRYCSNNCPYKVRRFNFLLYSDYETESLKLMRN
                     PEVSVRSRGVMEKCSYCSQRIEAVKIEADKDNRDIRDGEIRTACQQACPTDAIIFGNI
                     NDKTSRVAKIKTNERTYGVLADINTRPRTTYVAGVLNINEPLAQELGEYSS"
     gene            6092..7516
                     /gene="nrfD"
                     /locus_tag="VHE33_20160"
     CDS             6092..7516
                     /gene="nrfD"
                     /locus_tag="VHE33_20160"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HVT99824.1"
                     /translation="MATKDAPVNNPMIDPVTEEYRVIAPGHTFKSVTEEVSRIVLTSH
                     TPLGWWFGFLLAGSVVGILMTALTWLFLKGVGIWAITIPVAWGFAIINFVWWIGIGHA
                     GTLISAILLLFKQGWRNSINRFAEAMTIFAVVCAGMFPLIHVGRPWLGYWLFPYPSTM
                     NYWPQFRSPLLWDVFAVSTYATISVVFWYIGMIPDLGTLRDRSTSKAGQFFYGLLSVG
                     WRGSVRHWVRYETASLLLAGLATPLVLSVHSVISLDFSVALLPGWHTTIFPPYFVAGA
                     IYSGFAMVITLAVPIRKFYHLEDLITIRHIDNMNKVMLTTGLIVAYGYSTEVFMSWYS
                     ASHWEYFMMWNRMFGPMGWSYWTLITCNIATPQLLWSRKLRKNVAFTFVMSLIINTGM
                     WFERFVIVVTSLTRDYLPSSWGTYRATKWDYATFLGTLGLFTFLFFLFIRFLPMIPMS
                     ELRMMLPQSKVKEVHGEPLPEAGD"
     gene            7516..8058
                     /locus_tag="VHE33_20165"
     CDS             7516..8058
                     /locus_tag="VHE33_20165"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HVT99825.1"
                     /translation="MPLPEGTYALMAEFETPTQLVRAAKAAYGSGYRRMDCYTPYPVE
                     EAAEAIGFHRDEVSLVCLIGGLLGLVAMFSLETWISVWAYPLNIAGRPYYSWPAFVVP
                     AYEWTILWSGLSAAFGMLALCGLPKPYHPVFNAPNFRNGATSDKFFLCLEATDPKFEP
                     AETRSFLEKQAPLSVVEVEY"
     gene            8141..8896
                     /locus_tag="VHE33_20170"
     CDS             8141..8896
                     /locus_tag="VHE33_20170"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HVT99826.1"
                     /translation="MGGALGVLLLAGCRQDMQNQPRMFPQRSTTLFADGRSVRPQVEH
                     TVARGQLYEDEYFYTGLMNGKEADMMPFPVTATVMARGQERFNTYCTPCHSRVGNGAG
                     MIVQRGYKPAGNFHDAKRMSEPLSHYFYVMTNGYGAMPDYKAQIAPEDRWAIAAYIRA
                     LQLSQNAKQSDVPAGTEVQSLSSIAEKDGYPASYAGAWPMPSTAVTAKPEVGVVAGGP
                     AQAGGPAAATVPTAKSNTGATKTPPAKPSGAQR"
     gene            8896..10200
                     /locus_tag="VHE33_20175"
     CDS             8896..10200
                     /locus_tag="VHE33_20175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99827.1"
                     /translation="MANDSFSKGLPSTLGAPPFVDLWRRRALRVGLVSAVLAAGTGLL
                     AQMFDGTGLGWDHLLRGWLTGFVACFGFCFGGMALLMVQYLSGGKWGLIIRRPLEAMT
                     RTWPLIVLMWIPVAVLGATFTKLYPWAKYADWATALKNGQISGEMAHAIHWKGPMLNT
                     MNFYWVSLVCFAIFAGYILLLNRWSLQRDADPEPRVGYWRTRFENLSGFGVVLYAALL
                     FILVIYWVMSLDPTWYSTIYGFQYLVGQAFMVLPLAILTVMGLSKAEPIKSTLRVIEQ
                     HDLGKLCFALVMLNIYFAFGAFIIIWSGNSPEEIPWYLDRIRGGWGVIATLDVIFHWV
                     VPFSLLLSRDLKRIKNRLAVACCIMIFAGFFDLFWLIEPNFKDAARNLHFSWGILEYL
                     FVPAALYSFWLVNYFTELKKRPLIATNDPHLAEILEHDHVHA"
     gene            10181..>10653
                     /locus_tag="VHE33_20180"
     CDS             10181..>10653
                     /locus_tag="VHE33_20180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99828.1"
                     /translation="MTMSMHNPQSGKNRANLDPKAHLDEASVREGYEVTDANTGGIIV
                     FLVGLFLSVGVFFVFCFGMGKVINSGLDKADGPTNKWNAPNQIAPRKAIQSNPALEQE
                     NLAKLTQQFPTPRLELDDGNQDLSELHDREDLLLNNYSWVDKPGGAVRIPIERAM"
BASE COUNT         2037 a         2903 c         3677 g         2036 t
ORIGIN      
        1 ggccgaagcc ggccgagcag tcgctgaaag ccgggcagaa gatggggaca tccttttgat
       61 acgcggagag gacgatggag tcgacgccgc ccatttgggg ggtcttgccg ttggcttcaa
      121 gccaggcgcc catggcgcgg atgaactcgc gggagctgta gggccgcggc gcgagggagt
      181 cggcgacctt ctcggtggtc tcgtcgcagg cgcggagctc ttcttcgtcg atcagcgtgt
      241 cgtagatgcg atcgatcatc agctcgcgca gctcgccgtc ctgggttccg gacgtataga
      301 ggtcgtcggc gatgtagtgc tggaatccga gagcttcgaa gaagtcctga tcgacgatgt
      361 tggcgccggt ggagacgatg gcgtcaacca tgttgttgcg gatcaggtcg acgaagatct
      421 gcttgaggcc ggcggagatc agcgagccgg cgaggcagag gatgacgccg cagtcccgat
      481 cgcgcagcat gcgctcgtag atggaggcgg cccgggcgag gtcacgggag ctgtaggcca
      541 tggactgcat ggcgtcgacc agggcgacga cgttgtgctg cttgatgtcg atgtggcgga
      601 tgggttggtt caggagctct tttttcgaag tcataacaat gctcagaata acaaggaaaa
      661 gtgggcgaga ggttgggcgg cgatgaattg ctgcgggatg aaaacgagcc ggatggccgg
      721 caggttttag tcggaaagga ttagtcgggc aggaccatgg tcttgccggc gaggtcgcag
      781 gcgcgccaca tataccagct ggcgaccgag cgccagggac gccatttttc ggcgcggcgg
      841 gtgatctggt cggcggtggc gagcatggag gcgtcgaagg gtttggattt gggaaggcga
      901 aggaaggtga gggcgaagcc tttgcggacg ccgtagtcgg tgacggggag gacgtcgggg
      961 cggccgaggc ggaagatgag cagcatttca accgtccacg tgccgatgcc gcggacctca
     1021 gtgaggcgct cgatgatctc atcgtcgggc atgcggcgaa tggcggtgag cgttgggacg
     1081 gtgccgtcga tggtcttcgc ggcgaggtca cgcagggctt ttgtcttgga ggcggagagg
     1141 ccgcaggcgc ggagcatgtc gtcgggggcc tggagcaggt gttcgggtga gggatgcagc
     1201 tcgccaaaag cggtgagcag gcgtttgagg atggcggcgg cggctttgcc gtggagttgc
     1261 tgataaatga tggactcgag cagagcctcg aagggagagt gctggctctt gagtttgagc
     1321 gtgaagggta cgcgacggat gaggtcgccg agcttaggat cggcggcagc cagggtgctg
     1381 caggcgagag cggcgtcgta gcggacagcg cggcgtccat cgatcatgag agacagcgta
     1441 tagcgttcag cgcacagcgt acagaaccgc tttggaccca ttcatacgat cgcagcgaag
     1501 cgaagagccg gcgtggcgat tccgacgaca ggatacggac acggttacag attctggatc
     1561 tggcggcggt ggcgctgtgg agagggctga ggcggcggaa agtcagttgt cgaggccgcg
     1621 tggcgtggaa tggcgtgtgc gcaggacgtc attgttgatt cctgatgaac aaaaaatatt
     1681 ttcgacgagg accaggcggc ttgcaggagc ggcgcatgtg ccgctatagt ttttctttac
     1741 tgcaaaatgt gatgtaggtc acaatcggct gtgatccaga acacaatcag cacaatgtca
     1801 gggcgcaggg acttgccggg aaccgaagga ccgcgcgctg gcgataacag ggcaactggt
     1861 agaaacaccc gcatcctctg aggccgccgc ctgttcgtat tcgcagcgac ctgattccgc
     1921 agtcatcaag ccatcgaccc gcagcgattc accgcttcac agcaacgagg agacgcagga
     1981 cgcttcatgc cgcagatttt tgaccgcagc tcgaatgcgc tggcccggat gagcttgatt
     2041 ttgacggggc tgattgtaat ttccctgggt ctgacgctgg acgaagtgca gcggtctccc
     2101 tgggtgacgc gccaggggca gcgcgccgat cagccggttc ccttcagcca caagcatcac
     2161 gtggaagggc tgggactgca gtgccagtac tgccacacga cggtggagaa gtcgagctat
     2221 gcgggcattc ctccgaccaa gacctgcatg aactgccacg cgcagatctg gacgaacgcg
     2281 cagctgctgg aaccggtacg gcagagctgg gcgacgggag catcgattcc gtggacgagg
     2341 gtgcacgatt tgcccgacta cgtcttcttc aatcatgaaa ttcacgtgaa caagggcatc
     2401 gggtgctcat cgtgccacgg ccgtgtggac gagatgccca tcatgtatat gcagaactcg
     2461 ctgcagatgg agtggtgcct gaactgtcac cggaacccgg agaagaatct gcggccgacc
     2521 agcgagattt acaacatggc gtggcagggg gcgtccgagc tgcggccggt gtggtgtgcg
     2581 gcggaggatg ttccggcggg atcgccaacg gcagggggtg tgacctgcac ggaagagaat
     2641 ccaaaaatga acggcggggt ccagatggct tcgctcagcc ggccgccgac cgggctgcat
     2701 gcccaggcgg ggggcacagc gatcgggagc gaggacacga atcccatcca gctgccggca
     2761 ggggtgcact acacggagtt cacgagccag caggcgctgg gccaatttct ggagaagcac
     2821 tacgacattc gcaatccgcg tgagctgacc agctgcgagg tttgtcaccg atgaacagaa
     2881 acgagcgttc agcgttcggc gttcagcgtt caggcgagcc tgagcatgcc gcagtacccg
     2941 ggtcccagga gagtctggtg cagattgagc cgaagaagct gacgcttgct gaaattcggg
     3001 cgaagctcgc gaatcagaag ggcaaaaaat tctggcgttc ggtggacgag ctggcggatt
     3061 cgtcggagtt cgcggagctg gtgaacaagg aattcccttc ccaggcatcg gagtggatcg
     3121 atccggtttc gcggcgtggc ttcctgaagg tgatgggcgc ctcgatggcg ctggccggac
     3181 tggccggttg cacaaagcag ccggacgagc cgatttatcc gtatgtcacg gcgccaccgg
     3241 acctgacgct gggcaagccg gtgtattttg cctcggcgtt tccgtttacg accggcgcgg
     3301 tgccggtgct gatcaagagc gcggcgtacc ggccgatcaa ggtcgatggc aatccggagc
     3361 atccttatgt tcgcggcggg tccgacccga tcacgcaggc gacgctactg gatatgtacg
     3421 acccggatcg ctcgcagcac attttgtatc gcggcgagac gcggaactgg gcatcgtttc
     3481 tggcggcgtt tcgcagcatg ctggccgaca agaagccggc cggcgggcag ggcttgtact
     3541 tcctgagcgc gaccgttacc tcgccgacgt tggcagcgca gtggcagcag gcgcagaaga
     3601 attatccgca tgcgaagttt gtgcagtacg acccggcgaa ccgggacagc cactatcggg
     3661 cctcgcagca ggccttcggg caatttgtgg acgcgcagta ccggctggat caggcggacg
     3721 taattgtgtc gctcgatgcg gacttcctgg ggggcgcgca gttcccgggc ttccacaagc
     3781 tggcgcgcga ttacgcgcag aagcgcaagc tggacggcac cgatcagatg aaccggttgt
     3841 atgtcatcga gacggtgacg acgacgaccg ggttcaaggc cgatcaccgg ctggcggtga
     3901 agccgagcca gattgcggcg tttgcgcagg cgttggcggg cgcagtgggc ggcggaggat
     3961 cttcggccgg caactggagc gatcacgatg cgaagtatct gcagggcgtt gcggcggatc
     4021 tgaaggccaa cggcggcaag tgtgtggtga tcccgggcga atttgcgccg ccggaagtgc
     4081 atctggccgc cttcgcgatc aatcaggcgc tgggcaatgt cggcaagacg gtggtctata
     4141 ccgatccggt gaacccgatg ccgtcgatcc agtgcgacga tctgaaggcg ctggtcgcgg
     4201 acatgaacgc cggcaaggtc gagtggctgg tgatcctgaa tgcgaatccg ctgtactccg
     4261 cgccggcaga tctggatttc gagagcgcgt tcaataaggt caatacggtg gtgcatctcg
     4321 gttcgcactg ggatgaaacc ggccagcagg cgcactggca tatcaataac gcgcactatc
     4381 tcgagatgtg gtccgatgcc cgggcctacg acggcacggt aagcattgtg cagccgatga
     4441 tcgatccgat gtacggcggg cacagcgcgc acgagatcct gcagtcgatg ctcgacaatc
     4501 cggatgtctc ggcctacgac gcggtacgcg cgacctggct tggcaacggc ggcctgcttt
     4561 cgaagggtga tgcggaattc aactggcgca aggcgctgca cgatgggtgg atcgatggaa
     4621 cggcgttcgc gccaaagacc gtagcgccga agggcggact ggcgaccagc gccgggacag
     4681 ccgcggcgag cggggtggag gtggtgttcc ggcccgatgc gcatgtttac gacggtcgct
     4741 acgccaacct cggctggatg caggagattc cgcgtccggt aacgaacctg tcgtgggaca
     4801 acgcggcgca gatgagcttc gcgaccatgg agaagaacgg gctcgcggag aacgatgcgg
     4861 ttgagatcag cgtgggcggc aagaagatta ttggcggtgc gctggccgtt cccgggttgc
     4921 cggacgatgt ggtggttgtg acgctgggac aaggccgccg gatcggacga gtcgccggcg
     4981 gggtcggcta caacggctac ctgatccagc agtcgggtgc gccgtggatg ctggccggag
     5041 cgtcgctgcg gaagaccggc gacatctatg acatctgcgt aacgaagagc caccacatcg
     5101 atcagcgcag tgtggcgcac ggcggcgatg gttcgggcac gcattcgatc gaaggcaacg
     5161 agtcgctgga tcgcggaatc attcgctata cgacgctgac cgagtacaag aaggatccgg
     5221 aattcgcgca tgaaggggtg gcacgctgga cgccggagcc gaacgagagc atgttccccg
     5281 cgtatcggta cgacaagaac gcatggggga tgtcgatcga tctgaactcc tgcatcggct
     5341 gcaacgcctg cgtgacggca tgctttgccg agaacaacat tccggtggtt gggcgggagc
     5401 aggtcaagat cgggcgtctg atgcagtgga tccgcatcga cacgtatttc gagggcgatc
     5461 tggctgcacc gcgggcgcac ttccagccca tgacctgtca tcagtgcgag aacgcgccgt
     5521 gcgagcaggt ttgcccggtg ggcgcgaccg tgcactcgcc cgaagggctg aatctgatgg
     5581 tttacaaccg ctgcgtgggg acgcgctatt gctcgaacaa ctgtccctac aaggtgcggc
     5641 gattcaactt cctgctctac tccgattacg agacggagag cctgaagctg atgcgcaacc
     5701 ccgaggtttc ggtacggagc cgcggcgtga tggagaagtg cagctactgc agccagcgca
     5761 ttgaagcagt gaagatcgag gcggacaagg acaatcgcga tattcgcgac ggcgaaatcc
     5821 gcacggcatg ccagcaggcc tgcccgaccg acgcgatcat tttcgggaac atcaacgata
     5881 agacgagccg ggtagcgaag atcaagacga acgagcggac ctacggcgtg ctcgcggaca
     5941 tcaacacgcg gccgcgtacg acctatgtgg ccggcgtact gaacatcaac gaaccgctgg
     6001 cgcaggaact gggcgagtac tcgagctaga agcaggcaat agggaacagg caacaggcaa
     6061 cagctgagga atcggagaag gcaaagagac gatggcgacg aaagacgcac ccgtcaacaa
     6121 tccgatgatc gatccggtca cggaagaata ccgggttatc gcgccgggac acacgttcaa
     6181 gtcggtgacc gaagaggtct cgcggatcgt gctgaccagc catacgcccc tgggatggtg
     6241 gttcggattc ctgctggccg gttcggtggt gggcatcctg atgacggcgc tgacctggct
     6301 gttcctgaag ggcgtcggga tctgggccat cacgattccg gtcgcatggg gcttcgcgat
     6361 catcaatttc gtttggtgga tcggaatcgg ccacgccggg acgctgatct cggcgattct
     6421 gctgctgttc aagcagggat ggcgcaattc catcaaccgg ttcgccgagg cgatgacgat
     6481 cttcgccgtg gtgtgcgccg gtatgttccc gctgatccac gtgggccgtc cgtggctggg
     6541 gtactggctg ttcccgtatc cgagcacgat gaactactgg ccgcagttcc ggtcaccgct
     6601 gctgtgggac gtgttcgcgg tttcgaccta cgcgaccatc tcggtggtgt tctggtacat
     6661 cggcatgatc cccgatctgg ggacgctgcg cgatcgctcg acgtcgaaag ccgggcagtt
     6721 cttttatggg ctgctgtcag tgggctggcg cggttcggtg cggcactggg tgcggtacga
     6781 gaccgcctct ctgctgctgg ccggactggc gacgccgctg gtgctctcgg tgcacagcgt
     6841 catcagcctc gacttctcgg tcgcgctgct gccgggctgg cacacgacga ttttcccgcc
     6901 gtatttcgtt gcgggcgcca tctactccgg ctttgcgatg gtgatcacgc tggccgtgcc
     6961 catccgcaag ttctatcacc ttgaggatct gatcacgatc cgccacatcg acaacatgaa
     7021 caaggtgatg ctgacgacgg gactgattgt cgcgtacggc tattccaccg aagtgttcat
     7081 gagctggtat tcggcgagcc actgggaata cttcatgatg tggaaccgca tgttcggtcc
     7141 catggggtgg tcgtactgga cgctgatcac gtgcaatatc gcaacgccgc agctgttgtg
     7201 gtcgcgcaag ctgcgcaaga acgtggcctt caccttcgtc atgtcgctga tcatcaacac
     7261 cggcatgtgg ttcgagcgat tcgtgattgt ggtgaccagc ctgacgcgcg attatctgcc
     7321 gtcctcgtgg ggcacgtatc gcgcaacgaa atgggattac gcaacctttc tgggaacgct
     7381 ggggctgttc acgttcctgt tcttcctgtt cattcgcttc ctgcccatga ttccaatgtc
     7441 ggagctgcgc atgatgctgc cgcagtcgaa ggtgaaggaa gtgcatggcg aacctcttcc
     7501 ggaggcaggt gactgatgcc acttcctgag ggaacatacg cattgatggc ggagttcgag
     7561 acgccgacgc agctggtgcg ggcggcgaaa gcggcatacg gcagcggcta ccgccgtatg
     7621 gattgctaca cgccgtatcc ggtggaggaa gcggcggagg cgatcggctt ccatcgcgat
     7681 gaagtatcgc tggtgtgcct gatcggcggc ctgctgggcc tggtggcgat gttctcactg
     7741 gaaacgtgga tttcggtgtg ggcctatccg ctgaatatcg ccgggcggcc gtactattcc
     7801 tggccggcgt ttgtcgtccc cgcgtacgaa tggactattt tgtggtccgg gctctcggcg
     7861 gcgttcggga tgctggcgct gtgcgggctg cccaagccgt atcacccggt ttttaacgcg
     7921 ccgaatttcc gcaatggcgc gacctcggac aagttctttc tgtgtctgga ggcgacggac
     7981 ccaaagtttg agccggcgga gacgcgcagc tttctggaaa agcaggcgcc gttatcggtg
     8041 gtggaggtgg agtattaaga ccgcagcgta cagcgttcag cgtacagagc acggcggtcg
     8101 gcgctcggcg ttccgcgttc gcggtgtgcg ggctctgagc atgggtggcg cgctcggggt
     8161 gctgctgttg gccgggtgcc ggcaggacat gcagaatcag ccgaggatgt ttccgcagcg
     8221 cagcaccacg ctgttcgccg atggacgttc cgtacggccg caggtggagc acaccgtggc
     8281 gcgcgggcag ctttacgagg acgagtactt ctataccggg ctgatgaacg gcaaggaggc
     8341 ggacatgatg ccgtttcctg tgacggcgac ggtgatggcg cgcggacagg agcgcttcaa
     8401 cacctattgc acgccatgcc actcgcgtgt gggcaacggc gccggcatga tcgtgcagcg
     8461 tgggtataag ccggcgggaa atttccacga tgcgaagcgc atgtccgagc cgctgagcca
     8521 ctacttctat gtcatgacga acggttatgg cgccatgccg gattacaagg cgcagatcgc
     8581 gcctgaggat cgctgggcga ttgcggcgta catccgcgcg ctgcagctca gtcagaacgc
     8641 gaagcagagc gatgtgcccg cgggcaccga agtgcagagc ctgagctcga tcgcagagaa
     8701 ggacggatat ccggcgagct atgccggagc gtggccgatg ccgtcgaccg cggtgaccgc
     8761 gaagcccgag gtcggtgtgg tggccggcgg gccggcgcag gcaggcggac cggccgctgc
     8821 gacggttccc acagcaaaga gcaacacggg tgccacgaaa accccgccgg caaagccgtc
     8881 aggagcacag cgataatggc gaacgattca ttcagcaaag gattgcccag caccctcggg
     8941 gcgccgccat tcgtggatct gtggcggcgg cgcgcgctgc gcgtgggact ggtttccgct
     9001 gtgctggcag ccgggaccgg actgctcgcg cagatgttcg acggtaccgg gctcggatgg
     9061 gatcatctgc tgcgcggctg gctgaccggc tttgtcgctt gctttggctt ctgcttcggc
     9121 ggcatggcgc tgctgatggt gcagtatctc tccggtggca agtggggact gatcatccgg
     9181 cgtccgctgg aagcgatgac gcgcacctgg ccgctgatcg tgctgatgtg gattccggtg
     9241 gcggtgctgg gggcgacgtt cacgaagctc tacccatggg caaaatatgc ggactgggcc
     9301 accgcgctga agaacgggca gatttcgggg gaaatggcgc atgccatcca ctggaagggc
     9361 ccgatgctga acacgatgaa cttctactgg gtctcgctgg tctgcttcgc gattttcgcc
     9421 ggctatattc tgctgctcaa tcgctggtcg ctgcagcggg atgccgatcc tgagccgcgg
     9481 gtcggatact ggcgcacccg gtttgagaat ctgagtggat tcggcgtggt gctgtatgcc
     9541 gccctgctct tcatcctggt tatttactgg gtcatgtcgc tggacccgac gtggtactcg
     9601 acgatctacg gcttccagta cctggtgggc caggcgttca tggtgctgcc gctggcgatt
     9661 ctgacggtga tggggctgtc gaaagccgag cccattaaga gcacgctgcg cgtgatcgag
     9721 cagcacgatc tgggcaagct ctgcttcgcc ctggtgatgc tgaatatcta cttcgccttc
     9781 ggcgcgttca tcatcatctg gtcggggaac tcgccggaag agattccctg gtatctggat
     9841 cgcatccgcg gcgggtgggg cgtgatcgcg accctggacg tgatcttcca ctgggttgtt
     9901 cccttcagtt tgttgctgtc gcgcgacctg aagcggatca agaaccggct ggcggtggcc
     9961 tgctgcatca tgatctttgc cggattcttc gatctgttct ggctgatcga gccgaacttt
    10021 aaagacgcgg cgagaaatct gcacttcagc tggggaattc tggaatacct cttcgtgccg
    10081 gcggcgctgt acagcttctg gctggtgaat tacttcacgg agttgaagaa gcgtccgctg
    10141 attgcgacca acgatccgca tctggcggag attctggagc atgaccatgt ccatgcataa
    10201 cccgcagagc ggcaagaacc gcgcgaacct cgatcccaag gcgcatctgg acgaggcgtc
    10261 ggtacgcgaa ggctacgaag tcaccgatgc gaacaccggc gggatcatcg tttttctggt
    10321 cgggctgttt ctctccgtcg gcgtgttctt cgtcttctgc ttcggcatgg gcaaggtgat
    10381 caacagcggg ctggacaagg ctgacggccc caccaacaag tggaatgcac caaaccagat
    10441 cgcgccacgc aaggcgattc agtcgaaccc agcgctcgag caggaaaatc tggcaaagct
    10501 gacgcagcag tttccaacgc cgcgcctcga gctggacgac ggcaatcagg atctatccga
    10561 gctgcacgac cgcgaggatc tgctgctgaa caactacagc tgggtggaca aaccgggcgg
    10621 cgcggtgcgg attccgatcg aacgggcgat gga
//
LOCUS       DASZYN010000801         1778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_312095, whole genome shotgun sequence.
ACCESSION   DASZYN010000801 DASZYN010000000
VERSION     DASZYN010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312095"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VHE33_20185"
     CDS             complement(<1..648)
                     /locus_tag="VHE33_20185"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT99829.1"
                     /translation="MRNIWIICRRELSSYFASPIAWLLLAMYSIIFGWIFWNFLAEFI
                     KQGQDMMMMGQSQPMNVNEQIIRPLLGTSSFLGLFMIPLISMRLFAEEKRNGTIELLA
                     TSPVRDVEIIIGKWFSATLLYCWIIVFACINFVFLFRYGHPDWKPMLVTLLGVFLQGA
                     ALLAIGTFISTLTKNQIIAAAMTFGACLLLWVFSWVSSYDSSTWAQVFSYLSVITH"
     gene            complement(645..1679)
                     /locus_tag="VHE33_20190"
     CDS             complement(645..1679)
                     /locus_tag="VHE33_20190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HVT99830.1"
                     /translation="MITVTDLTKRYARTTAVDHISFSISKGDIVGFLGPNGAGKTTTM
                     RMLTCFFPPTEGKATVAGFDVFDQSMEVRRRIGYLPESPPLYLEMETIEYLRFVGRLK
                     GLSGTQLESRVAFVLERCNIGDVKTKLLSKLSKGYRQRVGLAQAIIHNPDVLILDEPT
                     AGLDPKQINETRDLIKSLAGDHTIILSTHILPEVEQTCESVIIINKGRLVATDSVHNL
                     QARARGAESVLVNVGVRSGEFPLSIVRDRLQRIPGVSGTALKEEFPTGAIFELESSKG
                     QLVRGDLARAIVESGWELNELRPSAMSLEEVFLQLTGSEAAAAPSAPIEPTPPAEAVG
                     ADAPGPEANA"
BASE COUNT          385 a          529 c          527 g          337 t
ORIGIN      
        1 gtgagtgatg acggagaggt aggagaagac ctgcgcccag gtcgacgagt catagctgct
       61 gacccaggaa aaaacccaca gcaagagaca ggcgccaaag gtcatcgcag ccgcgatgat
      121 ctgatttttc gtcagcgtcg aaataaacgt accgatggcc agcagcgccg cgccctgcag
      181 gaacacaccc agcagcgtca ccagcatcgg cttccagtcc ggatgcccat agcggaacag
      241 gaaaacaaaa ttgatgcacg cgaaaacgat gatccagcag tagagcagtg tggccgaaaa
      301 ccacttgccg atgatgatct caacatcgcg caccggtgac gtggccagta gctcgatcgt
      361 cccgttgcgc ttctcttcgg cgaaaagacg catgctgatg agcggaatca tgaacagccc
      421 gagaaaactc gaggtcccga gcaacggccg gataatctgc tcgttcacgt tcatgggctg
      481 ggactggccc atcatcatca tgtcctgccc ctgcttgatg aactcggcca gaaaattcca
      541 gaagatccat ccgaagataa tgctgtacat cgccagcagc agccaggcaa tgggcgaggc
      601 aaagtaactc gataactcgc ggcggcagat gatccagata tttctcatgc gttcgcctcc
      661 ggccctggtg catcggctcc gacagcctca gcaggcggcg ttggttctat cggagcgctg
      721 ggcgcagcag ctgcctcgct gccggtcaat tgcaggaaaa cttcttccag actcattgcc
      781 gacgggcgaa gctcgttcag ttcccagccg gattccacga tcgcgcgagc cagatcgccg
      841 cgcaccagtt gacccttgct gctttccagc tcgaagatcg cgccggttgg gaattcttcc
      901 ttcagagccg tgccgctgac cccgggaatc ctctgcaggc ggtcccgcac gatcgacaac
      961 ggaaactcgc ccgaccgcac gccaacattc accagcaccg attcggcccc gcgtgcgcga
     1021 gcctgcaggt tgtgcaccga gtcggtcgcg accagcctgc ccttgttgat gatgatgacc
     1081 gactcgcagg tctgctccac ttccggcaat atatgcgtgc tcaggatgat ggtgtgatcg
     1141 ccggcgaggc tcttgatcag gtcgcgggtc tcgttgatct gcttggggtc gagcccggcg
     1201 gtgggctcgt ccagaatcag cacgtcagga ttgtgaatga tcgcctgggc tagtcccacc
     1261 cgctgccgat agcctttgga aagcttgctc agcagcttcg tcttcacatc cccaatgttg
     1321 cagcgctcga gcacgaaggc cacgcggctc tccagctgcg tgcctgagag acctttgagt
     1381 cttcccacga agcgcagata ctcgatcgtc tccatctcca gatacagcgg cggcgattcc
     1441 ggcaggtagc cgatgcgcct gcggacttcc atggactgat cgaagacatc gaatccggca
     1501 accgtggcct tcccctccgt aggagggaag aagcaggtca gcatgcgcat ggtggtcgtc
     1561 ttgcccgcac cgttggggcc cagaaatccg acgatgtcgc ccttgctgat ggagaaagag
     1621 atgtggtcga cggcggtggt gcgggcgtat ctcttggtca ggtcggtaac agtaatcatg
     1681 tctgactgga agactgggtg gaactccggg gaagagaagt ggttagtaca gcatcaggct
     1741 gcgcagagcg cgtgggcggc gccggcagca gcaacaag
//
LOCUS       DASZYN010000802         7759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_879510, whole genome shotgun sequence.
ACCESSION   DASZYN010000802 DASZYN010000000
VERSION     DASZYN010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879510"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VHE33_20195"
     CDS             complement(<1..278)
                     /locus_tag="VHE33_20195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99831.1"
                     /translation="MKLVEFTGGEPLLQEKELVPLMERLLGQGYELMIETSGERPLER
                     VPRAVHKIVDVKCPGSGEGGSFRIANLDCLTERDEVKFVISNRGDYEFA"
     gene            complement(321..494)
                     /locus_tag="VHE33_20200"
     CDS             complement(321..494)
                     /locus_tag="VHE33_20200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99832.1"
                     /translation="MTKRNKGRAALYLIEIYKSIQGESSFAGVPCIFVRLAGCNLRCS
                     WCDSEYTFTGGYK"
     gene            complement(605..982)
                     /gene="queD"
                     /locus_tag="VHE33_20205"
     CDS             complement(605..982)
                     /gene="queD"
                     /locus_tag="VHE33_20205"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HVT99833.1"
                     /translation="MYEVTVERGFSSGHFLRNYKGKCENPHGHNYKVQITLCGETLDQ
                     AGLLLDFRDLKQVMRPVIDRLDHQMLNDLEPFTTLNPSAENLAKFFYDETNQQLREMT
                     QGRVRVKDCTIYETDTTTATYYE"
     gene            complement(1045..1338)
                     /locus_tag="VHE33_20210"
     CDS             complement(1045..1338)
                     /locus_tag="VHE33_20210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99834.1"
                     /translation="MARNLYILAGVLLLFAAICFVMAMTSGQFQPALPANGTLWKTTA
                     LFLTVGGLIIGLLGIMTALFEQVDRRSEERRIRQRGGRMRGSGGSSGPRLHGG"
     gene            1439..2815
                     /locus_tag="VHE33_20215"
     CDS             1439..2815
                     /locus_tag="VHE33_20215"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HVT99835.1"
                     /translation="MSLSIVILDDNPGSLELLSTALAQDGIAIHTASHPSEALDLIRQ
                     HHPQLVLTDLVMPEMSGLDVLNRVMEMAPATDVVLMTAHYTTETAVQAIRSGAADYLE
                     KPIRLPILRERVGRLIADARQRQQLGAEPEFEGIIGSSPRMAEMFARIRRIAPHYRSA
                     LIQGATGTGKDLVARALHTRSGVKGQYVVLNCSAVVETLFESELFGHVRGAFTGADRD
                     KPGLFEAANEGTLFLDEIGDMPLATQAKLLRALQNQEIQRVGSLQARRVNVRVIAATH
                     RNLRQAVADKLFREDLYYRLAMVEIPVPPLAERKEDLPRLIQYFVAKFSQQFGKQIRG
                     VSPRARLILERHPWPGNVRELENAIGHAAMMAEGNEIDAGDLPEALLGDPASGRSNDR
                     PDSNATASPAISSSASVPEDASLAEHERQLVVSALEKVQGNQSQAARALGIGRDALRY
                     KMKKYGLL"
     gene            complement(2980..3387)
                     /locus_tag="VHE33_20220"
     CDS             complement(2980..3387)
                     /locus_tag="VHE33_20220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT99836.1"
                     /translation="MATALRKPLILIADDRPSSRELLGLVLERAGYEVLEAENGEQAL
                     ERVRAGSPDLVLLDLQMPGMDGYGVLSELRAEARFASLPVLALTASAMRGDREKILEA
                     GFTDYLAKPAAPELVRETVARLLGEKRVSESAS"
     gene            complement(3476..6097)
                     /locus_tag="VHE33_20225"
     CDS             complement(3476..6097)
                     /locus_tag="VHE33_20225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HVT99837.1"
                     /translation="MRALVDNLDEAVVVLGHEGQVQFANDAAQRLYGIDPEGPRIAEW
                     SKTYGIYLPDHQTLWPAEELPGAVALRGQTVEGEQYVRPAGAIAGYWIRIRARPLFND
                     NGEVQGALLTWRDVTEEKKTSESQRRLLAIVENTPDLVALTDPTVKTSYLNRAGRALL
                     GLREDEEVGRYPVFDTDFEDQKDRFDSVILPALLRGEPWNGEFCLRHARTGDVITVDM
                     RAFGIFGPYGQLLGFASISRDITARRQAEETRQRLASIVEFSRDAIISESLDGVITSW
                     NRGAEQMFGYTAAEAVGKPITMLVWPGYEEDMRELLRRVGSEDIVEHYETLRRHKDGR
                     QLVVSLTLSPVRDSAGKLIGISKIARNITEQKKQEELADRQAQLLDQAYEPILVRDHR
                     DRIVYWNKGAERLYGWTASEAVGRVSHEILQTTFSEALEEITARLEQDGFWEGELVHR
                     TRRGARVTVLSRWIRELGVGEPHVMETNIDMSERQQRLAAEEATRAERRFRQLLEAAP
                     DAIVEVSADGKIVLINRVAEEMFGYVRDELLGQSVDVLVPDAIRRTHYRHRDSYLEQP
                     RTRPMGTGLDLHARRRDGTLFPVEISLSPIQTEGGMHVTAVIRDVTERKQAEKEVRRL
                     QQQYTGELEARNREAERANRLKSEFLASMSHELRTPLHTIIGFAELLQEGHDGDLNER
                     QQRFLGHIRRDSEHLLELINDVLDLSKIEAGQLTLKKEVYPLARSVGEALDAIRPGAI
                     AKGLEVGDLGERDAMVDADPLRVKEMLYNLLSNAVKFTPEGGRVWVEVREDAGFARVT
                     VGDTGVGISTDEQENIFDKFYQVGNTTRGVREGTGLGLSITKELVQMHGGWIEVDSRP
                     GEGSRFTFTLPIAAAEA"
     gene            6405..6833
                     /locus_tag="VHE33_20230"
     CDS             6405..6833
                     /locus_tag="VHE33_20230"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HVT99838.1"
                     /translation="MEVSLYHNGGSRFTAEARHHRVVVDQPVEDGATDQGVSPAELLL
                     ASLGGCVGQYVAQYLTTRALSTQGLAVRVRTQSSPRALRLGSFEVEVAAPGLTDSQLR
                     ALEASFPAGLVQNAIRFENQLQITATSTSPRKSPASRASR"
     gene            6900..>7759
                     /locus_tag="VHE33_20235"
     CDS             6900..>7759
                     /locus_tag="VHE33_20235"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVT99839.1"
                     /translation="MSSFSRRDFLKATAAAGAAATLGNVSLSAETRRATDLVPLGKSG
                     VKVGRLAFGTGSMSGAVQRELGQEGFNRLVRHAYDNGIRFFETADSYHDMHTMLGIAL
                     KGIPRDTYQLMTKVTTFDGTDPHQRIDELRKQINSDYFEVMLLHWQRGTEWPHETTGW
                     QNAIEKAQDRKIVLARGASVHGLPALQQVPTFNWLQVAMIRCNHNGTRMDTPIADGPG
                     RGNVPEVVERIHAARAAGQGVISMKLVGEGAFGKEDRQKAMRFAFQNAKVDAVTVGYK
                     NTQEIDEAIE"
BASE COUNT         1424 a         2979 c         1882 g         1474 t
ORIGIN      
        1 gcaaattcgt aatcgccacg gttgctgatg acaaacttca cctcatcccg ctccgtcagg
       61 caatccagat tcgcgatccg aaagctcccg ccctcgcctg acccgggaca cttcacatcc
      121 acaatcttgt gcaccgcccg cggaacccgc tccagcggcc gctccccgct ggtctcgatc
      181 atcagctcgt atccctggcc cagcagccgc tccatcagcg gaaccagctc cttctcctgc
      241 agcaacggct cgccgcccgt gaactccacc agcttcaccg gcgccagctt ctcgacctca
      301 gcaacgacct cgtcctcgga tcatttgtaa cccccggtga acgtatactc cgagtcgcac
      361 cacgagcagc gcagattgca ccccgccagc cgcacaaaga tgcacggcac gcccgcaaag
      421 ctcgattcgc cctggatgga tttatagatt tcgatcagat acaaagcggc gcgtccttta
      481 tttctttttg tcacgggatg cagcccgatt caccagccag gaaaaccatc cgaccaggcc
      541 catcagaatc gccgcgatgg cgagcccaac ccaccaatgg ttttccattt caatcccagc
      601 ccggctactc gtagtacgtc gccgttgtcg tgtccgtctc gtagatggtg cagtccttca
      661 cgcgcacccg cccctgcgtc atctcgcgca gctgctggtt cgtctcgtcg tagaaaaact
      721 tcgccagatt ctccgccgat gggttcagcg tcgtgaaggg ctccaggtcg ttcagcatct
      781 ggtgatcgag ccgatcgatc accggacgca tcacctgttt cagatcgcga aaatccagca
      841 gcagccccgc ctgatccagc gtctcgccgc acagcgtgat ctgcaccttg tagttatgtc
      901 catgcggatt ctcgcacttc cccttgtaat tccggagaaa gtgcccggac gaaaacccgc
      961 gctccacagt cacttcgtac atatcgtttg atcgtttcct gagcccagca aattcggcgc
     1021 gacctagcgc gcgctgttct cattctatcc gccgtgcaac cgcggaccag aactgccgcc
     1081 cgaaccccgc atccgcccgc ctcgctgtcg aatcctgcgc tcctccgagc gccgatccac
     1141 ctgctcgaac aacgccgtca taattcccag caacccgatg atcagtccac ccaccgtcag
     1201 gaacagagcc gtcgtcttcc acagcgtccc attcgcgggc agcgccggct gaaactgccc
     1261 ggacgtcatc gccatcacga aacaaatcgc ggcaaacaac aacagcacgc ccgccagaat
     1321 gtacagattc cgcgccacag ctcagtctac cgcctgcctt ccctcttccg gctcccgcat
     1381 cccgctgtcc tgtaccctgt cttctgtacg ctgtaggctg aacgctgaac gctgctccat
     1441 gtccctctcc atcgtcatcc tcgacgacaa ccctggcagt ctcgagctgc tctccaccgc
     1501 gctcgcccag gacggcatcg ccatccacac cgcctcgcat ccctctgaag cgctggacct
     1561 catccgccag caccacccgc agctcgtcct caccgatctc gtcatgcctg aaatgtccgg
     1621 cctcgacgtc ctcaaccggg tcatggaaat ggctcccgcc accgacgtcg tcctcatgac
     1681 cgcgcactac actaccgaaa ccgccgttca ggccatccgc agcggcgccg ccgactacct
     1741 cgaaaagccg attcgcctcc ccatcctgcg cgaacgggtc ggccgcctca tcgcggacgc
     1801 acgccagcgc cagcaactcg gcgccgagcc ggagttcgaa ggcatcatcg gctccagccc
     1861 gcgcatggcc gaaatgttcg cccgcatccg ccgcatcgct ccgcactacc gctctgcgct
     1921 catccaggga gccaccggca ccggcaagga tctcgtcgct cgcgcgctcc acacccgcag
     1981 cggcgtcaaa ggccagtacg ttgttctcaa ctgctccgca gtcgtcgaaa ctctcttcga
     2041 atccgaactt ttcggccacg tccgcggagc cttcaccggc gccgaccgtg acaagcccgg
     2101 cctcttcgaa gccgccaacg aaggcaccct cttcctcgac gaaatcggcg acatgcctct
     2161 cgccacccag gccaaacttc tgcgcgcgct tcagaatcag gaaatccagc gcgtcggctc
     2221 cctccaggca cgcagggtca atgtccgcgt catcgccgcc acgcatcgca acctgcgcca
     2281 ggccgttgcc gacaagctct tccgcgaaga cctctactac cgcctcgcca tggtcgagat
     2341 ccctgtccct ccgctcgccg aacgtaaaga agacctgccg cgcctcatcc agtacttcgt
     2401 cgcgaaattc tcgcagcagt tcggcaaaca gattcgcgga gtctcaccgc gcgcccgtct
     2461 catcctcgag cgccacccgt ggcctggcaa tgtccgtgag ctggagaacg ccatcggcca
     2521 cgccgccatg atggccgaag gcaatgaaat cgacgccggc gatcttcctg aggctctcct
     2581 cggcgaccca gcctctggcc gttcaaacga ccgccccgat tcaaacgcga cggccagccc
     2641 ggcaatctcg tcctcggcca gcgtccccga agacgcctct ctcgccgagc acgaacgcca
     2701 actcgtcgtc agcgctcttg aaaaagtgca gggcaatcag tcccaggccg cgcgtgccct
     2761 cggcatcggc cgcgacgccc tccgctacaa aatgaagaag tacggcctgc tttaagagtc
     2821 ggaagttatg agccattagt tgtgagtgcg cctccggctc aggatgtccg aatcagaact
     2881 cgcaactcac aactcacaac tcacactcac aactcgtaac ccgaaactca aaactcgctg
     2941 cccacaactc gcagtcggct cgcctcggct ggcagttcgc taactcgctg actcgctgac
     3001 tcgcttttct cccagcaatc gcgctaccgt ctcgcgcacc agctccggtg cagccggctt
     3061 cgccagataa tccgtgaagc ccgcctcaag aatcttctcc cggtccccgc gcatggcgct
     3121 cgcggttagc gccagcaccg gcagcgacgc aaagcgagcc tccgcccgca gctccgacag
     3181 caccccgtaa ccatccattc ccggcatctg caggtcgagc aacaccaggt ccggactccc
     3241 cgcccgcacc cgctccagcg cctgctcgcc gttctcggcc tccagtacct catatccggc
     3301 gcgttccaga accaggccca gcagctcgcg gctgcttggc cgatcatcgg caatcaggat
     3361 caacggtttt ctcaaagcag tcgccatagc gcgcccttgc ctccagcagg ctcagcattc
     3421 cgagcacact caagcactca tccagttcgg ggccctgggg atcgatgcgg atatcctacg
     3481 cctcagctgc ggcaatgggt agggtgaagg tgaaccggct accctctccc ggcctgctgt
     3541 cgacctcgat ccatccgccg tgcatctgca caagctcttt tgtaatcgac agacccagcc
     3601 ccgtaccctc ccgcaccccg cgcgttgtgt ttcccacctg gtagaatttg tcgaaaatat
     3661 tctcctgctc gtcggtcgaa atcccaactc ctgtatcccc gaccgtcacc cgcgcaaagc
     3721 ctgcatcttc ccttacctcc acccacaccc gcccgccttc cggtgtgaac ttcaccgcat
     3781 tgctcagaag gttgtacagc atctccttca cacgcagcgg atcggcatcc accatcgcat
     3841 ctcgctcgcc cagatctcct acctccagcc ccttcgcgat cgcccccggt cgaatggcat
     3901 ccagcgcctc tcccacgctc cgcgccagcg ggtatacctc tttcttcagc gtcagctgcc
     3961 ccgcctcgat cttgctcaga tccagcacgt cgttgatcag ctcaagcaga tgttctgaat
     4021 cccgccgaat gtgcccaaga aaccgctgtt gccgctcgtt cagatcgccg tcatgcccct
     4081 cctgcaacag ctccgcaaat ccgatgatgg tgtgcagggg cgtgcggagt tcgtggctca
     4141 tactcgccag gaactcgctc ttcagccggt tcgcccgctc tgcctcccga ttgcgcgcct
     4201 ccagctcgcc ggtgtattgc tgctgcagcc gtcgaacctc cttctccgcc tgcttccgtt
     4261 ccgtcacgtc ccggatcact gccgtcacat gcatcccgcc ttcggtttgg atcgggctca
     4321 ggctgatctc gacggggaac aacgtcccgt ctctccgccg cgcgtgcagg tccagtcccg
     4381 tccccatggg tcgtgtcctc ggctgctcga gatagctgtc ccgatgccgg tagtgtgtgc
     4441 gtcgaatcgc atccggaacc agcacatcga ccgactgccc cagcaactcg tcgcgtacgt
     4501 acccaaacat ctcctccgcc acgcgattga tcagcacaat ctttccatcc gccgacactt
     4561 ccacgatcgc atctggcgcc gcctccagaa gctgccggaa ccgacgctcg gcccgcgtcg
     4621 cctcctccgc cgccagccgc tgctgccgct ccgacatgtc gatgttcgtc tccatcacat
     4681 gcggctctcc aacccccagc tcgcggatcc accggctcag caccgttacc cgcgcgcccc
     4741 ggcgtgtccg gtgcaccagc tcgccttccc agaacccgtc ctgctccagc ctggctgtaa
     4801 tctcctccag cgcttcggaa aaggtcgtct gcaggatttc gtggctcacc cgccccaccg
     4861 cctcgctcgc cgtccatccg tacagccgct ccgcaccctt attccagtaa acgatccgat
     4921 cccgatgatc ccgcaccaga atcggctcat aagcctgatc caggagctga gcctgccgat
     4981 ccgcgagttc ctcctgcttc ttctgctccg taatattccg cgcaatcttc gaaattccga
     5041 tcagcttccc cgcgctgtcc cgcacaggcg acaacgtcag ggacaccacc agctgtcgac
     5101 cgtccttgtg ccgtcgcagg gtctcgtaat gttccacgat gtcttccgat cccacccgcc
     5161 gcagcaactc ccgcatgtcc tcttcgtatc ctggccacac cagcatcgtg atcggctttc
     5221 ccaccgcctc cgctgccgtg tacccaaaca tctgttccgc cccgcggttc cagcttgtga
     5281 tgaccccgtc cagcgattcg ctgatgatcg cgtcgcgcga aaactccaca atcgacgcca
     5341 gccgctgtct cgtctcctcg gcctgccgcc gcgcggtaat gtcccggctg atgcttgcaa
     5401 accccagcaa ctgcccatac ggaccgaaaa tgccgaacgc ccgcatgtcg accgtgatca
     5461 cgtcgcccgt ccgtgcgtgc cgcaggcaga actccccgtt ccacggctct ccgcgcagga
     5521 gcgcgggcag gatgacggaa tcgaaccgat ccttttggtc ttcgaagtct gtatcgaaaa
     5581 ccgggtagcg gcccacttcc tcgtcttctc gcagccccag cagcgccctc cccgcgcgat
     5641 tcagatagct ggtcttcacc gtcggatccg tcagagccac aaggtccggc gtgttctcaa
     5701 cgatcgccag caatctcctc tggctctccg aagtcttctt ctcctcggtg acgtcgcgcc
     5761 acgtcagcag cgcaccctgc acttcgccgt tgtcgttgaa cagaggccgc gcccgaatcc
     5821 ggatccagta cccggcaatt gctcccgccg gccgcacata ctgctcgccc tcgacggtct
     5881 gccctcgtaa agccaccgcc cccggaagtt cttccgctgg ccaaagggtc tggtgatcgg
     5941 ggagatagat gccgtacgtc ttgctccact cggctattct cggcccttcc ggatcgatgc
     6001 cgtaaaggcg ctgagccgcg tcattcgcga actgcacctg cccttcatgc cccagcacca
     6061 caaccgcctc gtccaggttg tccactagcg cgcgcatcag atccgtttcg ctgcctgcca
     6121 acgaaccagg aactccggcg tttgccgctc cggcgccagc gcgcctggga gcatcacccg
     6181 cccctccaga attgggattt cggctcttca tgggaaacag gctgatagtg tacccgcgac
     6241 tcctgacgcc tcgcacccgg ctgcgtcatt tcacgcatcc gccctcgatt cgagtcccat
     6301 cacgcacctg cattctccca acatcctgcg gtaaacccgg ccttccagcg ctttctcaca
     6361 ttggccatcc gcttgcatct ccgcctgcaa accaggagtg tgctttggaa gtctcgctgt
     6421 atcacaatgg cggttcgcgt ttcaccgccg aagcccgcca ccaccgcgtt gtcgttgacc
     6481 agcccgtcga agacggcgcc accgaccagg gtgtgtcgcc cgctgaactt ctcctcgcct
     6541 ccctcggcgg ctgtgtcggc cagtatgtcg cgcagtatct caccacgcgc gccctctcca
     6601 cccagggcct tgccgtccgg gttcgaacgc agtcctcccc tcgtgccctg cgcctcggca
     6661 gttttgaggt cgaagtcgcc gctcccggcc tcaccgattc ccagctccgc gccctcgaag
     6721 catcattccc cgccggtctc gtgcaaaatg ccatccgctt cgaaaatcaa ctccagatca
     6781 ccgccacatc aacctcgccc cgaaaatccc ccgccagccg cgcttcccgc tgaccgactc
     6841 cagctgaccg cgtcaccagc tgttccgtgc tacgctgact gcatcggagg agccccagca
     6901 tgtcctcttt ctctcgtcgc gatttcctca aagccactgc cgccgcaggc gccgccgcca
     6961 ctctcggcaa cgtttccctc tcggctgaaa cgcgccgtgc caccgacctc gtccctctcg
     7021 gcaaatccgg cgtcaaagtc gggcgtctcg ccttcggcac cggcagcatg agcggagccg
     7081 tccagcgcga gctgggccag gaaggcttca atcgtctcgt ccgccacgcg tacgacaacg
     7141 gcatccgttt cttcgaaacc gccgactcct atcacgacat gcacaccatg ctcggcatcg
     7201 cgctgaaggg cattccgcgc gacacctacc agctcatgac caaggtgacg accttcgacg
     7261 gaaccgatcc ccaccagcgc atcgacgagt tgcgcaaaca aatcaactcc gactatttcg
     7321 aagtcatgct cctgcactgg cagcgcggca ccgagtggcc tcacgaaacc acaggctggc
     7381 agaacgccat cgaaaaagct caggaccgca aaattgtcct cgcgcgcggt gcatcggtcc
     7441 acggtcttcc cgcccttcag caggtcccga ctttcaactg gctccaggtt gccatgatcc
     7501 gctgcaacca taacggcacc cgtatggaca cgccgattgc cgacggccct ggccgcggca
     7561 acgttcccga agtcgtcgag cgcatccacg ccgcccgcgc cgccggacag ggcgtcatca
     7621 gcatgaagct cgtcggcgaa ggcgccttcg gcaaggaaga ccgccagaaa gccatgcgct
     7681 tcgcgtttca aaacgcgaag gtagatgcgg tcaccgtcgg ctacaagaac acccaggaaa
     7741 tcgacgaggc catcgagaa
//
LOCUS       DASZYN010000803         2032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_570067, whole genome shotgun sequence.
ACCESSION   DASZYN010000803 DASZYN010000000
VERSION     DASZYN010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570067"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1191
                     /locus_tag="VHE33_20240"
     CDS             109..1191
                     /locus_tag="VHE33_20240"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HVT99840.1"
                     /translation="MIKVKRFRIALCCAAASAVLLQAPPISGSARDSRQTENVPPAKT
                     GAPQDPGAQSYAQHCAICHGEQRQGNLPGFPPLLGISHRMTNDQITELIHSGKGRMPG
                     FPSLQSGEVEALLHFLETAEPAAQVASNSTEHTSLSAAGEALFQQNCAFCHGRDAQGG
                     ETGPDLTQSRLVRRDRTGDDIAKVIHEGRPNNKMPAFNFSTQEVRSIIAFIHAREAEA
                     VAHPGGRRGVAVADLQTGNVDAGKRYFDGSGGCNKCHSPTGDLAGIASKFEGLELEER
                     MLYPRDATSRVTVTLPSGEKFSGVLAYRDEFTVALRDANGIYRSWPAGKVHATVDAPV
                     EAHVALFSKYTDDDIHNLMAYLQTLR"
     gene            1221..>2032
                     /locus_tag="VHE33_20245"
     CDS             1221..>2032
                     /locus_tag="VHE33_20245"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVT99841.1"
                     /translation="MKSLNYRCLLIFAALFAGLSAPGFLAAQDLENAQLLHPTPDSWP
                     MYHGDYSGRRHVPFTQITPQNVGDLALAWAFQTNQPAQIKSSPLLVDGILYFTVPDNV
                     WAVDARSGHMVWHYNRVSTKGDHIGHRGVAMYKDWLFFTTPDAHLISLNAKDGTVRWD
                     KIIADVDKGYWSTMAPLVVKNHVIAGVGGDLDNLTGYLRSFDPDTGAIQWQWNATPPS
                     GTPNATTGGMTWITGTYDPDLNLLYWGTGNPTPVLNGKPRPGDDLYTCSIVAL"
BASE COUNT          409 a          646 c          602 g          375 t
ORIGIN      
        1 atgcaactca tcaagtactc gacctagcgt ggtgaaggaa tttcagaaac tccacgcgaa
       61 ccgaagccgt tccgccgttc gaaaattaaa agtttcggag ttgttgcatt gatcaaggta
      121 aagaggtttc ggatcgcgct ctgttgcgca gcggcaagtg cagttctcct ccaggctcct
      181 cccatctcgg gctcagcgcg cgattcccgg caaacggaga acgttccacc ggcaaaaacg
      241 ggcgctccgc aggatcccgg cgcgcaatcg tacgcgcagc actgcgccat ttgtcacggt
      301 gaacagcgac aggggaatct gcccggcttc cctcccctgc tgggtatctc gcaccgtatg
      361 acgaatgacc agatcacgga actgatccac tccgggaagg ggcgcatgcc cggctttccg
      421 tccctgcaga gcggtgaggt ggaggcgctg ctgcatttcc ttgagactgc ggagcccgct
      481 gcccaggtgg ccagcaacag cacggagcac acgagtctgt cggcggcagg cgaagctctc
      541 tttcagcaga actgcgcctt ttgccacggt cgcgatgcgc aggggggaga gacgggccct
      601 gatctcacgc aatcgcggct tgtgcgacgc gatcgcaccg gagacgacat cgcgaaggtg
      661 atccacgaag gccggcccaa taacaagatg cccgccttca atttctccac gcaggaagtt
      721 cgcagcatta tcgcgttcat ccacgcgcgc gaagcggagg cggtggcgca tccgggcggc
      781 cgccgcggtg tcgccgtcgc cgatctgcaa accggcaacg tggacgcggg caaacgctac
      841 tttgacggct ccggcggctg caacaaatgc cactcaccga ccggcgacct ggcgggcatt
      901 gcctcaaaat tcgagggact ggagcttgag gaacggatgc tgtatccgcg cgacgccacg
      961 agtcgggtca cggtcacgct gccctccggc gagaaattct caggcgtgct cgcctatcgg
     1021 gacgagttca ccgtggcgct gcgcgatgcg aacggaatct accgctcctg gccagcggga
     1081 aaagttcatg cgaccgtcga tgcgcctgtc gaggcgcacg tcgcgctttt cagcaagtac
     1141 accgacgacg acattcacaa cctgatggct tatctgcaaa cgctgcgctg attgctgcca
     1201 gtggacctga ggttcaattc atgaagtctc tgaactatcg atgccttctc atcttcgctg
     1261 cgctcttcgc aggtctctcc gcgccgggat tcctggccgc gcaggatctc gagaatgcgc
     1321 aactgctgca ccccacgcca gactcgtggc cgatgtatca cggcgactac tccggacggc
     1381 ggcacgttcc ctttacgcag atcacaccgc agaatgtcgg cgatcttgcg ctggcttggg
     1441 cattccagac caaccagccc gcacagatca agtcctcgcc cctgctggtg gatggcatcc
     1501 tgtacttcac ggttcccgac aacgtgtggg ccgtcgacgc gcgttccggt cacatggtct
     1561 ggcactacaa ccgcgtctcc accaaaggcg accatattgg gcatcgcggc gtcgccatgt
     1621 ataaggactg gctcttcttc accacgcctg acgcgcatct gatctcgctc aacgccaaag
     1681 acggcacggt ccggtgggac aagatcatcg ccgatgtgga caagggctac tggagcacga
     1741 tggcgccgct ggttgtgaag aatcacgtga tcgccggagt cggcggagac ctggacaacc
     1801 tcacgggata tctgcgctcg ttcgatcccg acacgggcgc cattcaatgg caatggaacg
     1861 cgacgcctcc gagcggcacg cccaacgcga cgaccggcgg catgacgtgg atcacgggca
     1921 cgtacgatcc cgatctgaat cttctgtatt ggggaacggg caatccgact ccggtgctca
     1981 acggcaaacc gcgtccgggc gacgaccttt acacgtgcag catcgttgcg ct
//
LOCUS       DASZYN010000804         3831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_252002, whole genome shotgun sequence.
ACCESSION   DASZYN010000804 DASZYN010000000
VERSION     DASZYN010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252002"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VHE33_20250"
     CDS             complement(<1..461)
                     /locus_tag="VHE33_20250"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HVT99842.1"
                     /translation="MQTFSSDGHSGRIRFAFCAAAALTLLLVASPLRAQVVATYDFED
                     GTAQGWTSFYNASTPVNTASIAHAGAHSLSTTTSAAGTGGPQINLTSVLQAGAQYTIT
                     GWVRLASGETATDANFTIQRSDTSPSCSGGTCYDTIGAYQTGVTADGWVQIG"
     gene            794..2998
                     /locus_tag="VHE33_20255"
     CDS             794..2998
                     /locus_tag="VHE33_20255"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HVT99843.1"
                     /translation="MRLCLALAVCALLALSGCGSREGGSGSTPPPPPPSTNPDQRADA
                     ILAQMTQAEKLQMVHGGLTLENSVGPHNAAGWVPGISRLGVPELLYADLVTINPSTAL
                     PSALASAASWDLSEAHKYGQVIGSEMRAFGMNVCLTGDMNLTSREPRDGRTFETRGED
                     PLLAGMVEAAHLEGIEEQHLIAGIKHVAMNDQETARFTANAVVDERAARESDLLAFEI
                     ALKTSGAQMVMCAYNAVNGTPSCANSHLLTQTVKGDWGFPGFVLSDWYATPSSVPAAL
                     AGLDQEQPGGYMFNGVWTPESLQAALQNGDLPATRLDDMVHRILRAMFASGLFDQPAT
                     VQPIDAAGDAAIAQEMEEQGAVLLKNTGQLPLNSSALPSIAVIGSHADVGVLSGGGSA
                     QTFPVGGAALTLPPACPPYTPPPGGAACTGASQIYDPSSPLTAIRAHAPNATVAWNDG
                     SDPAAAAALAASSTVAVVLVSEWESEGMDRVDLSFSNNQDALVSAVAAANPHTVVVLE
                     NGGPLLMPWLSNVSAVVEAWYPGQKGGEAVANLLFGTVNPSGKLPMTFPGAVADLPRP
                     VIPVPSPPDSTSAFAVDYTIDGLNVGYKWFESQGRVPLFPFGFGLSYTTFSISNTQLT
                     TGTPASSGFQVSFDLQNTGAVAGAEVPQVYLALPSAAGEAKRLVGWSKVRLDPGAKQH
                     VTINVDAGGVSHPLSIWDTTTNAWVVPGGHYTVYLGNSSAAPDLKMAGSFDL"
     gene            complement(3025..3552)
                     /locus_tag="VHE33_20260"
     CDS             complement(3025..3552)
                     /locus_tag="VHE33_20260"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HVT99844.1"
                     /translation="MPSRFFAVLCAPVFIAVLTGCGYHTLNSAAHLPSTVHTLAIPSF
                     KNNTQSYHTQAVFTEAVIREFNNRSSLRIVSGEDPDADATLRGTILTFHINPLTYDNT
                     TGQSSSYLVTITTKLELVDRNGKVIWQQPSYLFRQQYEQIQDLASFIQEDSAATRRLA
                     REFASAAVADILESF"
BASE COUNT          672 a         1034 c         1382 g          743 t
ORIGIN      
        1 ccgatctgga cccagccgtc cgcggtcact cctgtttgat aggcaccgat ggtgtcgtag
       61 caggtgccgc cgctgcacga gggcgaggtg tcagagcgct ggatggtgaa gttggcgtcg
      121 gtcgcggtct cgccgctggc aagacgcacc catccggtga tggtgtactg cgctcccgcc
      181 tgcagcacgc tggtgaggtt gatctgcgga ccgccggtac cggcggcgct ggtggtggtg
      241 gagaggctgt gggcgcctgc gtgcgcgatc gaggcggtgt tcacgggtgt cgacgcgttg
      301 tagaacgagg tccatccctg tgcggtgccg tcttcgaagt cgtaggtcgc caccacctgc
      361 gcgcgcaacg ggctggccac gagcagcagg gtgagcgctg cggcggcaca aaaagcgaag
      421 cgaatccgtc ctgagtggcc atcactggaa aaggtctgca tgggaagctc ctgtctttaa
      481 tcctgatcgc taacaaaatt gagctgttgg aagacgggct ggcgtgattg ggcagagatc
      541 acggggctct gctgaaacgg ggcaaagtat aggcacctaa caaaatgagt gtcaagctgg
      601 ctgctgtgga ttgtgcctgg cggtggagat aacgcgccgg gaggggacaa attttgcaag
      661 gaaaaagcgg ccggcagcga accccggggc tatcatgctg gtggacgaaa actggttgat
      721 acgagccggt actctccagc tccgcaactg ctgtccccgg ggaaagtttc ggtggtccga
      781 tgaggtaccg gggatgagac tttgtctggc acttgcggtt tgcgcgctgc ttgctctcag
      841 cggttgcggg agtcgagagg ggggctcggg cagcacgccg ccgccacctc cgccttcgac
      901 caatcctgac cagcgcgcgg acgccatcct ggcgcaaatg actcaggccg aaaagctgca
      961 gatggtacac gggggtctca cgctcgagaa ttcggtggga ccgcataacg cagccggctg
     1021 ggtgcctgga atttcacggt tgggcgtccc ggagctgctg tacgcggacc tggtgaccat
     1081 caatccttct acggctctgc cgtcggcact ggcgagcgcg gccagctggg atttgagcga
     1141 ggcgcacaag tacggacagg tgatcggcag cgagatgcgg gcctttggca tgaacgtgtg
     1201 cctgacggga gacatgaacc tgacctcacg cgagcctcgg gacgggcgca cgtttgaaac
     1261 acgaggcgag gatccgctgc tggccggaat ggtagaggca gcgcatctgg agggcatcga
     1321 ggaacagcac ctcattgccg gaatcaagca cgtggcgatg aacgaccagg agactgcgcg
     1381 ctttacggcg aacgcggtgg tggacgagcg cgctgcacgg gaaagcgatt tgctggcgtt
     1441 tgaaattgct ctgaagactt ccggcgcgca gatggtgatg tgcgcctaca acgcggtgaa
     1501 cggcacgcct tcgtgcgcca acagccacct gctcacgcag acggtgaagg gcgactgggg
     1561 ctttccggga tttgtgctct cggactggta tgccacgccc agttcggtac cggcggcgct
     1621 ggcggggctg gaccaggagc aaccgggcgg ctatatgttc aacggcgtgt ggacgccgga
     1681 gagcctgcag gcggcgctgc agaatggcga tttgccggcg acgcgcctgg acgacatggt
     1741 gcaccggatt ctgcgcgcca tgtttgcgtc aggactcttc gatcagcctg cgacggtgca
     1801 gccgatcgac gcggccggag atgccgcgat tgcgcaggag atggaggaac agggcgccgt
     1861 gctgctgaag aatacggggc agctgccgct gaacagctct gccctgccct ccattgcggt
     1921 gattggctcg catgccgacg ttggggtact ttccggcggc ggttcggcgc agacttttcc
     1981 ggttggcggt gctgcactga cgcttcctcc tgcatgcccg ccttacacgc cgccgccggg
     2041 cggagcggcg tgcacgggag cttctcaaat ctatgatcct tcttcgccgc tgacggcgat
     2101 tcgcgctcat gcgcccaacg cgaccgtggc ctggaacgat ggatcggatc cggcggcggc
     2161 cgcggcgctg gctgcgtcct ccacggtggc ggttgtgttg gtaagcgagt gggagagcga
     2221 gggaatggat cgggtggacc tgagtttttc gaataaccag gacgcgctgg tgagtgcggt
     2281 cgcggctgcc aatccgcata ccgttgttgt gctggaaaat ggcggaccgc tgctgatgcc
     2341 gtggctgtcg aatgtgagcg cggtggtgga ggcgtggtat ccgggacaga agggcggtga
     2401 ggcggtcgcg aaccttctgt tcgggacggt gaatccatcg gggaaactgc ccatgacgtt
     2461 tccaggggcg gttgcagatc tgccgcggcc ggtcattccc gttcccagcc caccggattc
     2521 caccagcgcg tttgcggtgg actacaccat cgacgggctc aacgtcggat acaagtggtt
     2581 cgagagccag gggagggtgc cgctgtttcc gtttgggttt ggcctttctt acacgacgtt
     2641 ctcgatctcc aatacgcaac tgaccacggg gactccggct tcttcgggat ttcaggtgag
     2701 tttcgatctc cagaacacgg gcgccgtggc cggggcggag gttccgcagg tttatctcgc
     2761 gcttccgagt gccgcaggtg aggcaaagcg gctggtgggc tggagcaagg tgcggctcga
     2821 cccgggggcg aaacagcatg tgaccatcaa cgtggatgcg ggcggcgtct cgcatccgct
     2881 ttcgatctgg gatacgacga cgaatgcctg ggtggttcct gggggacact acacggtgta
     2941 cctcggcaac tcgtcggcag cgccggattt gaagatggcg ggaagcttcg atttgtaagg
     3001 gctcggacga gccttgtttc ccgcttagaa ggattcgagg atgtcggcga ccgctgcgga
     3061 ggcgaactcg cgcgcgaggc ggcgcgttgc ggcggaatct tcctggatga agctggcgag
     3121 gtcctgaatc tgctcgtatt gctggcggaa gagataggag ggctgctgcc agatcacttt
     3181 tccatttcgg tcaaccagct caagcttcgt agtgatcgtg accaggtagc tggagctctg
     3241 gcccgtggtg ttgtcgtagg tgagcggatt gatgtggaag gtgaggatgg ttccacgcag
     3301 ggtcgcgtcg gcatccgggt cttcgccgga gacgatgcgg agcgaactgc ggttgttgaa
     3361 ttcgcgaatg acggcttcag tgaagacggc ctgggtgtgg tacgactgcg tgttgttttt
     3421 aaaggagggg atagccaggg tgtggacggt gctgggcaga tgggcggcgg agttgagggt
     3481 gtggtagccg catccggtga ggacggcgat gaagaccggg gcacacagaa ccgcaaagaa
     3541 gcgtgagggc atgactgtgg gtattttcgc agagcgcgcc ttcggcgcgt taggcccacc
     3601 cttctcgcgt gcaacaccag cacgcgacca gggtggggca tccggggtgt ttgcgttagg
     3661 ccccccgcgc caggggcgca cccggaactc cggacagaga aaaggccagc tccgaagagc
     3721 tggccttgtt tcttcaacga actgcgatta cgcggggctg gggctgcctt cgggcaggct
     3781 gccggtgcgt cccggttcgg gtttgagcac ctgctgcacg tcggtggttg g
//
LOCUS       DASZYN010000805         4388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_312587, whole genome shotgun sequence.
ACCESSION   DASZYN010000805 DASZYN010000000
VERSION     DASZYN010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312587"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VHE33_20265"
     CDS             <1..729
                     /locus_tag="VHE33_20265"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HVT99845.1"
                     /translation="FVALNKQTFPLFENTRKGDFREVTTPSGMRDLSLGMGGYGPALY
                     DFDNDGWKDLFVTRGHVESLPLPGAEIAQDNTVFRSLGSSGKWAALTGEAGLDAAPPA
                     RHRGCALGDFDGDGRVDLVVSALSAPAEIWMNRSDHSGHWLDVALRGTRSNRDAIGAR
                     IKVVSPTVGAQYNHMTTSLCYASSSDGPVHFGLGPDTKAETVEIHWPSGQVTRITNVK
                     ADQVLKLTELPLTTEHDPGSTARR"
     gene            943..2541
                     /locus_tag="VHE33_20270"
     CDS             943..2541
                     /locus_tag="VHE33_20270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HVT99846.1"
                     /translation="MAELHYDEANWTPSHNPWLVALTVTLATFMEVLDTSIANVALPH
                     MAGTLGASQEEATWVLTSYLVASAVILPISGWLMARMGRKRFYMSCVMLFTICSMLCG
                     LAPSLPLLVLFRVLQGVGGGGLAPSEQAILADTFPVSKRGQAFAVYGMAVVVAPAIGP
                     TLGGWITDNFNWHWIFFINLPVGLLSLFLTQRMVEDPPYLKEEQKTSRRVDFMGLGLI
                     SIGVGCLEFVLDKGQEKDWFGDSTITAFAIIAVCTLVCFVWWEWQHDDPIVDLRLLKN
                     RNFGTAVFLQFVLGMVLFGTTVLIPQFLQVLLGYTAERAGMVLSPGALVLMLTMPIAG
                     RLVGSGKLDPRLLVAMGYAITAAGLFNLTRLDLGVSFGQITLWRAYQVMGLSFVFIPI
                     STLNYLGVPRNKNNQISSFSNFARNIGGSVGTALLTTFLQRTQQSHIASLSTHAVGGS
                     VQYSRFVEMTQQALMRLGQSADAARQIALGNAWQSLNRQAQMLSYQNAFWGLALIIAC
                     LIPLPFIMRRPPRTQRVREEMAGH"
     gene            2601..3527
                     /locus_tag="VHE33_20275"
     CDS             2601..3527
                     /locus_tag="VHE33_20275"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVT99847.1"
                     /translation="MDFENLRTFLEVSRLKSFSRAAQKLLRTQPAISAQIRTLEQEVG
                     ARLFDRDGGKVTFTAAGRLFEPFAEYCVERQRHILLAIAEQERSPRGEISISANEATH
                     LYVLPQVFAQFRQQYPRVMLSVVRAERLRTLENVVNREVDFGIVSMPVRDTRLAVDLI
                     HRDELALVTPRGHALAGRGAVRLQEAVRYPMLLPTHGRRREQLDELFRVNEIVPRVAM
                     EVESSEMLKRYIGVGLGLGFLPRTNVVEEERSGALHALALEGVRLARELALVYRKDRQ
                     LSKAAREFLEIASQPVRKRESEGGQGRARSTV"
     gene            3669..4214
                     /locus_tag="VHE33_20280"
     CDS             3669..4214
                     /locus_tag="VHE33_20280"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF013979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HVT99848.1"
                     /translation="MVPRRLFFTKGVGKHKERLTSFELALRDAGIAAQNLVRVSSIFP
                     PQAKLIGRKEGLKYLSPGEVVFAVVAENSTREPHRLVVSSIGVAIPSDRNTYGYLSEH
                     HSFGETEDQAGEYAEELAAEMLATTLDVDFDPDKSWDEKKEIYRISNKIVRTANVTQS
                     AIGDKRGLWTTTIAAAILIFE"
BASE COUNT          838 a         1198 c         1452 g          900 t
ORIGIN      
        1 tttgttgcgc tgaacaagca gacctttccg ctcttcgaaa acacgcgcaa gggagatttt
       61 cgcgaggtga ccacgccgag cgggatgcgc gatctgagcc tggggatggg cggttacgga
      121 ccggctctct acgacttcga caacgacggg tggaaggacc tgttcgtgac gcggggccat
      181 gtggaatcgc tgcctctgcc tggagcagag atcgcacagg acaatacggt gtttcgcagc
      241 ctgggatcga gtgggaagtg ggcagcactg acgggggagg cgggtctcga tgcggctccg
      301 ccggcgcggc atcgcggatg tgctttggga gacttcgatg gcgacgggcg cgtggacttg
      361 gtggtctcgg ccctatccgc accagcggaa atctggatga accgcagcga tcactccggg
      421 cactggctgg acgtcgccct gcgcggcacg cgcagcaatc gtgacgcaat cggggcgcgg
      481 atcaaggtgg tgagtccgac agtgggtgcg cagtacaacc acatgacgac gagcctgtgc
      541 tacgccagct ccagcgatgg tcccgtgcac tttggcctgg gaccggatac gaaggcggag
      601 acagtggaga ttcactggcc gtcgggacaa gtcacgagaa taacgaatgt gaaggcggat
      661 caggtgctga agctcacgga gttgcccctt acgaccgaac acgatccggg atctaccgcc
      721 cgccgctgat tctcgcctga ccgaaccggt tcgtttggtt aacggctggg caaaacccgg
      781 caaactgaac cctggcgtgc aactgctaat ctaggaactg tcccactcag caattcattt
      841 cccccgcgcc cgaggcgcat cagccgctct ctttgaagat tttggggatt cagggaccct
      901 cgcgaagggc ggggagattt ctgcaccaca cggaccgaac ctttggcgga actgcattac
      961 gacgaggcca actggacgcc cagccacaat ccgtggctgg tggcgctcac cgttacgctg
     1021 gcgaccttca tggaggtgct ggacacctcc atcgccaatg tcgcgctgcc tcacatggcc
     1081 ggcacgctgg gcgcgagcca ggaggaggcc acgtgggtgc tgaccagtta tctggtcgcg
     1141 agcgccgtta tcctgcccat ctccggctgg ctgatggcgc gcatggggcg gaaacgcttc
     1201 tacatgagct gcgtgatgct gttcacgatt tgctccatgc tgtgcggcct ggcgccctcg
     1261 ctgcctctgc tcgtgttgtt ccgcgtgctg cagggtgtgg gcggcggcgg cctggctccc
     1321 agcgagcagg ctattctggc cgacaccttc ccggtgagca agcgcggcca ggcgtttgca
     1381 gtctacggaa tggcagtcgt ggtggcgccc gccattgggc cgacgctggg cggatggatc
     1441 accgacaatt tcaactggca ctggattttt ttcatcaact tgcccgtcgg gctgctgtcg
     1501 ttgttcctga ctcagcgcat ggtggaagat ccgccgtatc tgaaggaaga acagaagacg
     1561 agccgccgag tggatttcat ggggctcgga ctcatctcga tcggcgtggg ctgcctcgaa
     1621 ttcgtactcg acaagggcca ggagaaggat tggttcggcg actcgacgat caccgcgttt
     1681 gcgatcatcg ccgtgtgtac gctggtgtgt tttgtctggt gggagtggca acatgacgat
     1741 ccgattgtcg atctgaggct gttgaagaat cgcaactttg gcaccgcggt ttttctgcaa
     1801 tttgttctcg gcatggtttt gtttggcacc accgtgctga ttccgcaatt cctgcaggta
     1861 ttgctgggtt acacggcaga gcgcgccggg atggttctgt cgccaggcgc cctggtgctg
     1921 atgctgacca tgcccatcgc cggccggctc gtgggatccg gaaaactcga cccgcgattg
     1981 ctggttgcta tgggctatgc catcaccgcg gcagggctgt tcaatttgac gcggctcgat
     2041 ttgggtgtgt cgtttgggca gatcacgctg tggcgcgcgt atcaggtcat gggcttgtcg
     2101 ttcgtcttca ttcccatcag cactctgaat tatctgggcg tgccgcgcaa caagaacaat
     2161 cagatttcga gtttctcgaa tttcgcacgc aacatcggtg gatcggtggg cacggcgctg
     2221 ctgaccacgt tcctgcagcg aacccagcag tcgcatattg ccagcctgag cacgcatgcc
     2281 gtgggcggca gcgtgcagta ttcgcggttt gtggagatga cgcagcaggc cctaatgcgg
     2341 ctggggcaga gcgcagacgc cgccaggcag atcgccctcg ggaatgcgtg gcagagcctg
     2401 aaccgccagg cacagatgct gagctaccaa aacgcattct gggggctggc cctgatcatc
     2461 gcgtgtctga tcccgctgcc attcatcatg cggcgaccgc cgcgcacgca gcgtgtccgc
     2521 gaagaaatgg cgggccacta acgagtggtg aaattccttg tggccgctcc cctgagccgc
     2581 atattcttga ggcattcgca atggactttg aaaatcttcg aacctttctc gaggtcagcc
     2641 gcctgaagag cttttcgcgt gcggcccaga aactgcttcg cacccagccg gcaatctcgg
     2701 cgcagattcg cactctggaa caagaagtgg gcgcgcggtt gttcgaccgc gatggcggaa
     2761 aggtgacctt taccgcagcc ggacggttgt tcgagccgtt tgccgagtac tgcgtggaac
     2821 ggcagcggca cattcttctg gccatcgcgg aacaggagcg ctcgccgcga ggggaaattt
     2881 cgatcagcgc gaacgaggcc acccatttgt atgtgctgcc gcaggttttc gcacagtttc
     2941 ggcagcagta tccgcgtgtg atgctgagcg tggtgcgcgc cgagcggctg cgcacgctgg
     3001 agaacgtggt caaccgggaa gtcgatttcg gcatcgtctc gatgcccgtg cgcgacacgc
     3061 ggctggcggt ggacctgatt catcgggacg aactcgcgct ggtcacgccg cgggggcacg
     3121 ctctggcggg acgcggtgcg gttcggctgc aggaagcggt gcgttatccc atgctcctgc
     3181 ccacgcatgg caggcgcaga gagcaactgg acgagttgtt ccgggtgaac gagattgtgc
     3241 cgcgcgtggc catggaggtg gagtcgagcg agatgctgaa acgctacatc ggcgtggggc
     3301 tggggctggg ctttttgccg cgcaccaatg tggtcgaaga ggaacgctcg ggcgcgctgc
     3361 acgccctggc gctggaaggt gtgcgcctgg cccgcgagct ggctctggtc taccggaaag
     3421 atcgccagct ttcgaaagca gcacgggaat ttctggaaat tgcgagccag cccgtgagaa
     3481 agcgcgaatc ggaaggcgga caggggcgag cgcgcagtac cgtatgattg aaagcgatgt
     3541 taattccttt tcatgggcgt atccggcagg catttctgtg ttaattttca gcggggacat
     3601 agttttcggg caggtgagtg cgccgggatt tcaaaaggac ttggaggagt tgagaattgg
     3661 gaaaaagcat ggttccgagg cggctgttct tcaccaaagg agtggggaaa cacaaggagc
     3721 ggctgacctc atttgagctg gcgttgcggg atgccggcat tgcggcgcag aatctggtgc
     3781 gcgtttcatc catctttcct ccgcaggcaa agctgattgg gcgcaaagag gggctgaaat
     3841 acctcagccc aggcgaggtt gtctttgccg tggtcgcaga gaattccaca cgcgagccgc
     3901 accggctggt ggtatcgagt atcggcgtcg cgattccgag tgatcgtaac acttacggtt
     3961 acttaagcga gcatcacagc ttcggcgaga ccgaggatca ggcgggtgag tacgcggaag
     4021 agctggcagc agagatgctg gccacaacgc tggacgtgga tttcgatccc gacaagagct
     4081 gggatgagaa aaaagaaatt taccgcattt cgaacaagat tgtgcgcacc gcaaacgtca
     4141 cgcagtcggc gattggcgac aaacgcgggc tgtggacgac gacgattgcg gcggcgattc
     4201 tgattttcga ataggtttac ggagtgtatc aggcaggcgt gggccaaccg gcctgcgcct
     4261 tttgcttgtt tctgcacgac acgtaaccag agaagataca aatatggcag agctcggtag
     4321 ttttcgcgtt ggtctggtgc agatgagctg tggaccggat ccggacgcca atctggaaaa
     4381 ggccgcgg
//
LOCUS       DASZYN010000806         5987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_381528, whole genome shotgun sequence.
ACCESSION   DASZYN010000806 DASZYN010000000
VERSION     DASZYN010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381528"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..660)
                     /locus_tag="VHE33_20285"
     CDS             complement(292..660)
                     /locus_tag="VHE33_20285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99849.1"
                     /translation="MIDKQYPGSISARKLVIETALLNVITAYSAEEAIETLIRFPGVD
                     GIVLDTEVKGMTCRHLIERLRAVRSDLPVITVSPSGHDPCGAEDFHVSSYDPQDLLAQ
                     LSHLCPKEIRQAAEESDFPR"
     gene            820..2538
                     /locus_tag="VHE33_20290"
     CDS             820..2538
                     /locus_tag="VHE33_20290"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HVT99850.1"
                     /translation="MSMVRNLPIARKFLWAFGIISLLCVGLAVYSFITMRGIISLSID
                     VRENDIPSILHLANMRNALNTVRRTDLSLLTCASDECVNRNTAKRQKAIPTFEAEAKG
                     YEPLIADSGERQIYDRFKSNFEKYLDASNRAAEMEATGQRDQANALITSTNTAGYFQD
                     ALDAGDQAMQLNAREGNEAATAVVDASRRTNWISIVMTLAIVGFSVLIGTQLNRFVTP
                     RIRNVMKMVQRLADKDMTAYVRPTATDEIGRMGEMLNGSVANLREVLRSVAQSTQTLS
                     AATTEISARASESATNADTQSGMTTQIATAAQEMTATIGEISRNAESAASASRVSAET
                     ATRGGEVMQAASSTMEKIAAATSSVSERIGSLAHRSEEIGNVVNVIQEISEQTNLLAL
                     NAAIEAARAGEHGRGFAVVAGEVRRLAERTKAATEEIAGTIRSIQEETRQTIEVMDSS
                     RAAVDSGLEETAKARRSLDEIIESSRQVEHQIQLIASAATEQTAASEEISESAGRISH
                     LAVQNTQGAGEAVTALKDLSRLAVDLDTMIRQFQLEEASGGAGGSFRASRESAAAVGL
                     QPAHSL"
     gene            2737..3372
                     /locus_tag="VHE33_20295"
     CDS             2737..3372
                     /locus_tag="VHE33_20295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99851.1"
                     /translation="MVSLRQVALFAITVSFLSGCSSTSTSSSPAPQPAATTQAAAAPQ
                     IVTGKTAFWEMYKTAHAWASDAQPMRLTMKELPGYKNEAGKAGMWEATFGSGSLRSYR
                     TFTYAIAAAPPNIYKGVDAGMALPWGGPTRDAMPIDTSMFTIDSDAAYTAAAAEAAPV
                     LKTKPDLKVTDFEVGATSKFPTPVWYILWGSKKAGFAAVVDGSSGKVMKGK"
     gene            complement(3416..3913)
                     /locus_tag="VHE33_20300"
     CDS             complement(3416..3913)
                     /locus_tag="VHE33_20300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99852.1"
                     /translation="MNSRPVESLGPKETRVLFQAMGFFFHLVLCLVAWGIALGAITLL
                     HPSYVPPLVTLIVSFVVPLAAGFGFTKLHPTESATLPWMMGLIWWMVWGLHVLDMPTG
                     PMACYHCGATDKLWLTFLSLNSDSGLLDGQGRFIATWPAVAMIGYSIGAKLGLRNADI
                     PFESN"
     gene            4013..5083
                     /locus_tag="VHE33_20305"
     CDS             4013..5083
                     /locus_tag="VHE33_20305"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HVT99853.1"
                     /translation="MLLEGVFPAMTTPFYPDGRLYLRKLEQNVEHYSRTPCAGMVVLG
                     STGEAVMLGDEETRDVLRHAAGAAAPDKVLLAGVARESLSETLRLAEYAAEKQYDAVL
                     VRTPNFYTPSYGLTSGALPMLTYYRAIADRSPLPVVLYSIPRFTNVELPLEAVGELAQ
                     HPNIIGIKDSSGSLERIQALLAATRNAPKRSVLVTPVFTAITGRMMMAETEAAAASDF
                     VAADVLAGVPSVAVAPLVVRTMRKKDVSFQVLSGSADTLLSSLEAGASGAVLAFAACA
                     PQACQEVYTAWKENDPVVAHEKQRRLIDAARVVTGKYSIAGIKHACDLNGYYGGVPRI
                     PLLPLNADQKAEVATAMGDLRN"
     gene            5200..>5987
                     /locus_tag="VHE33_20310"
     CDS             5200..>5987
                     /locus_tag="VHE33_20310"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HVT99854.1"
                     /translation="MFLKQYYLGCLAHASYLLGDEASGTGIVVDPQRDVEQYLADAAA
                     NGLTIRHVFLSHFHADFVAGHLELRDRCGATIHLGARGKAEYPFVAMSDGDVLDFPRL
                     RLEVLETPGHTIESISILVYDKQRDAEHPYAVLTGDTLFIGDVGRPDLRASLGWTAAE
                     LGSHLYDSVQNRLLPLPDATLVYPAHGAGSLCGKNISKETVSTMGEQRRFNYALQPMS
                     RDQFIAIVTADQPDAPPYFTWDAVLNTRERTTLDTNLGLVLQPL"
BASE COUNT         1185 a         1776 c         1920 g         1106 t
ORIGIN      
        1 gaagacccgc gaagcagaac gtcaacacaa aaccgacgaa atgcgcgaca atgttgcgca
       61 gaaggtagat gacgccaaaa accaggcgaa cgagaagatc gatgagttca aggaacggga
      121 acgtcagcgc cgttcggcct aatcgaacct gctgtcgacg tttctgatcc ctaaagaccc
      181 gggggtgcag tgccatccgg cctgatcgcc cccagggtgc atctacctca ccgggctcgc
      241 agccaacgct gcgagccctc ttttttgtct gcagcagacc tggagcggca gtcagcgggg
      301 aaaatcgctt tcctcggcgg cttggcggat ttctttcgga cacagatgac ttagctgagc
      361 gaggagatcc tgcggatcat aggaggagac gtggaagtcc tccgcgccgc atggatcatg
      421 gccgctcgga gaaaccgtga tcaccggcag atcgctgcgg accgcccgta gccgctcgat
      481 gaggtggcgg caggtcattc ctttcacttc tgtgtcaagg acgataccgt ccaccccggg
      541 gaaacggata agcgtctcga tggcctcttc ggcgctgtag gcggtaatca cgttgagcag
      601 ggcggtctcg atcaccagtt tgcgggccga gatgctgccc gggtactgct tgtcgatcac
      661 cagaaagcaa ggtcggatca ttcccggtga gatgcgaccc gggccggaac agatgaatct
      721 gcatcgattc gctgtcatga atggcgctgc cgcgccgata ggggtgctgg ggggcggaat
      781 cggccacacc atcccgtccg acggaaggat ctagcgctta tgtcgatggt tcgtaacctg
      841 cccatcgccc ggaagttcct ctgggcattc ggcatcatca gcctgttgtg cgtggggctg
      901 gctgtgtaca gcttcatcac gatgcgcgga attatttccc tgagcatcga tgtgcgggag
      961 aatgacatcc cgtcgattct tcatctggca aatatgcgga acgctctcaa cacggtgcgc
     1021 aggaccgatc tgagcctgct cacgtgcgcc tccgatgagt gcgtgaaccg gaacacagcc
     1081 aagcgtcaaa aggctatacc cacgttcgag gctgaagcga agggatacga gccgttgatt
     1141 gccgattcgg gtgaacggca gatctacgac cggtttaaat cgaattttga gaaatatctc
     1201 gacgccagca atcgcgccgc ggagatggaa gccaccggtc agagagatca ggcgaatgcg
     1261 ctgatcacct cgacaaacac cgccggttat ttccaggacg cactggacgc tggcgatcag
     1321 gccatgcagt tgaatgcccg cgaaggcaat gaggccgcga ccgccgtggt cgacgcgagc
     1381 cggcgcacga actggatcag cattgtgatg acgctggcca tcgtgggttt ctcggttctg
     1441 atcggaaccc agctgaaccg cttcgtgacg ccgcgaatcc gcaatgtgat gaagatggtg
     1501 cagaggctgg ccgacaagga catgacggcg tacgttcgtc cgacagcgac cgatgagatc
     1561 ggacggatgg gggaaatgct gaacggcagc gtggccaacc tgcgcgaggt gctgcggtcg
     1621 gtggcgcaga gtacgcagac tctgtcggcg gccaccaccg agatcagcgc gcgcgccagc
     1681 gagagcgcga caaacgccga tacccagtcc gggatgacaa cccagatcgc gacggcggcg
     1741 caggagatga cggcgacgat tggcgaaatc agccgcaacg cggagagcgc tgccagcgcc
     1801 agccgtgtct cggcggagac ggcgacccgg ggcggtgaag tcatgcaggc ggcctcctcc
     1861 acgatggaga agatagccgc cgcaaccagc tcggtgtcgg agcgaatcgg ctctctggcg
     1921 catcggtccg aagagatcgg gaatgtggtg aacgtgattc aggagatcag cgaacagacc
     1981 aacctgctgg cactcaacgc ggccatcgag gcggcgcggg ccggggagca tggacgcgga
     2041 ttcgcggtgg tcgcaggaga agtgcgccgc ctcgcggagc ggaccaaggc ggcaacggag
     2101 gagatcgccg ggacgatccg cagcattcag gaggagactc gtcagaccat cgaggtgatg
     2161 gacagcagcc gcgcggcggt ggactcgggt ctggaggaaa ccgcgaaggc gcgccggagc
     2221 ctcgacgaaa ttatcgaatc gtcacggcag gtcgagcacc agatccagct gatcgcgtcg
     2281 gctgcaaccg agcagacggc ggcttcggag gaaatctccg agtctgccgg gcgcatttcc
     2341 cacctggcgg tgcagaatac gcagggagcg ggtgaagcgg tgactgcgct gaaggatctt
     2401 tctcgtctgg cggtcgatct ggatacgatg atccgccagt tccagctgga ggaagccagc
     2461 ggcggagcgg gagggtcgtt cagggcatcg cgtgaatcgg ctgcggccgt tgggctccaa
     2521 cccgcgcact cgctttgatc tgtgcttcaa cgccagcgcc gctcgggcat gccgcccggg
     2581 cggcggttct cttcaggttc ccagaagaaa tatcgtgaca gaggtcacat cgttttcttt
     2641 gcccgaccgg cgatcctact gtcgcggacg gcgtccgggg tctatacttg ctccccggac
     2701 gaaggtcgcg tctcgatcgt gaggtggagg acaaagatgg tttctctgcg ccaagtcgcg
     2761 ttgttcgcaa tcacagtgtc gttcctgtcc gggtgttcca gcaccagcac cagcagcagt
     2821 ccggccccgc agccggccgc caccacccag gccgccgcgg cgcctcagat tgtcacgggc
     2881 aaaacagcct tctgggaaat gtacaagacc gcgcacgcct gggcttccga tgcgcagccg
     2941 atgcggctga cgatgaagga gcttcctggc tataagaatg aagctggcaa ggccggcatg
     3001 tgggaggcaa cgttcgggtc cggcagcctg cgcagctacc gaacctttac ctacgcaatc
     3061 gccgccgcgc cgccgaatat ctacaaaggc gtggacgccg ggatggccct gccctggggc
     3121 ggcccaaccc gggatgcaat gccgatcgat acttcgatgt tcaccatcga ttccgacgcc
     3181 gcctatacgg ccgctgcggc ggaagcggcg ccggtgctga agacgaaacc ggacctgaag
     3241 gtcacggact tcgaggtggg ggccacctcg aagtttccaa cgccggtctg gtacatcctg
     3301 tgggggtcga agaaagccgg gtttgccgcc gtggtggatg gcagcagcgg caaggtgatg
     3361 aagggcaaat aacgggtttg gctccccgga accggtcggg ccatctgcgg aacgctcagt
     3421 tgctctcgaa ggggatgtcg gcgttccgca atcccagctt cgcgccgatg gagtatccga
     3481 tcatggccac ggctggccag gtggcgatga aacgcccctg gccgtcgagc aggccgctgt
     3541 cggaattcag gctcaggaag gtaagccaga gcttgtccgt tgctccgcag tggtagcagg
     3601 ccatggggcc ggtgggcatg tcgagcacgt gcagccccca gaccatccac cagatcagtc
     3661 ccatcatcca cggaagggtg gcactctcgg tggggtgcag cttcgtgaag ccgaagccgg
     3721 ctgccagcgg aaccacaaag gacacaatca gcgtgaccag cggcggcacg taagaagggt
     3781 gcaacagcgt gatcgcgccc agggcaattc cccacgcgac gaggcagagc accagatgaa
     3841 agaaaaaacc catcgcctgg aacaggacgc gggtttcctt gggtcccaga ctctcgaccg
     3901 gccgcgaatt cacagcacaa ctccgaatcg catctgtccc ccagactaac gcgctggcgc
     3961 aggagttgca gttccggctc gcacttttct ggtaactcat tacaatcgtt ttatgcttct
     4021 ggaaggcgtt tttccggcca tgacgacgcc gttctatcct gacggccgtc tttacctgcg
     4081 caaactggag caaaacgtcg agcactactc gcgcacgccc tgtgccggca tggtggttct
     4141 cggctccacc ggcgaagccg tgatgctggg cgacgaggag acgcgggatg ttctgcgcca
     4201 tgcggccggc gccgcggctc ccgataaggt tcttctggcg ggagtggcca gggaaagtct
     4261 ctcggaaacg ctgcgtctgg ctgaatatgc ggcggaaaag cagtacgacg cggttctggt
     4321 tcggacgccg aacttctaca cgccttccta cggtctgacc agcggcgcgc tgccgatgct
     4381 gacctactat cgcgcgatcg ccgaccgttc accgctgccc gtggttctgt acagcattcc
     4441 acgcttcacg aacgtcgaac tgccgctgga agccgtcggg gaactggccc agcatccgaa
     4501 tatcatcggc atcaaggatt cttcgggcag tctggaacgc atccaggcct tgctcgccgc
     4561 gacccgcaat gcaccgaaac gcagtgttct cgtgacgccg gtctttaccg ccattaccgg
     4621 ccgcatgatg atggcggaga ccgaggccgc agcggcgtcg gactttgtcg ctgcagacgt
     4681 gctggccggc gtgccatcgg ttgccgtggc gccgcttgtc gtcaggacga tgcggaagaa
     4741 agatgtgagt ttccaggtgc tttccggttc ggctgatact ttgctctctt cgctcgaggc
     4801 cggcgcgtcc ggcgccgttc tggcgttcgc tgcatgcgcg ccgcaagcct gccaggaggt
     4861 gtacacagcc tggaaagaga acgacccggt cgttgcgcat gaaaagcagc ggcgcctgat
     4921 cgatgctgcg cgcgtggtca cgggtaaata cagcatcgcg ggcatcaagc atgcctgcga
     4981 cctgaacggc tactatggcg gcgttcctcg tattccgctt ctgccgctga acgcggatca
     5041 aaaagccgag gtggcgacgg cgatgggtga tcttcgaaat tagcttcatc gccgcaggtt
     5101 ctttggcagg cgttttctcc gcgattcagg cgctgggcct gggtactctc tgcataatgc
     5161 cttgaccgcc gtccctccca cgggtgaagc ttctacgcaa tgttcctgaa acagtactat
     5221 ctcggatgtt tggcccacgc gtcgtacctt ctgggcgatg aggcctccgg cacaggaatc
     5281 gtcgtcgatc cccagcgcga cgttgagcag tatctcgccg atgccgcggc gaacggtctt
     5341 acgattcgcc atgtcttttt gagccacttc catgccgatt ttgtggcggg gcatctggaa
     5401 ctgcgggatc gatgcggcgc gacgattcat ctgggcgccc gggggaaagc agaatatccg
     5461 tttgtcgcca tgagcgacgg cgatgttctc gactttcctc ggcttcgtct tgaggttctc
     5521 gaaacgccgg ggcacaccat cgagtcgatt tcgatcctgg tttatgacaa acagcgcgat
     5581 gccgagcatc cctatgccgt tcttaccggc gatacgctgt tcatcggcga tgtgggacgg
     5641 cctgatctgc gcgcctcgct tgggtggacc gctgcagagc tgggaagcca tctctacgat
     5701 tcggtgcaga accggctctt gccgctcccc gatgccacgc ttgtttatcc cgcgcatgga
     5761 gcgggctcgc tgtgcggcaa gaacatctcg aaggagacgg tttcaacgat gggcgagcag
     5821 cggcggttca actacgcgct gcagccgatg agccgcgatc agttcatcgc catcgtaacc
     5881 gccgatcagc ccgatgcgcc gccctacttc acctgggatg cggtgctgaa tacgcgggag
     5941 cggaccacgc tggatacgaa tctcggactg gttttgcagc cgctgga
//
LOCUS       DASZYN010000807         2125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1273535, whole genome shotgun sequence.
ACCESSION   DASZYN010000807 DASZYN010000000
VERSION     DASZYN010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273535"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VHE33_20315"
     CDS             complement(<1..735)
                     /locus_tag="VHE33_20315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99855.1"
                     /translation="MRRRLRLSATVLGCCIFFSICLTAKAARPSDTVKPPAAPGGGES
                     IPIPGPLRSFLRMAGISQEAEPEDVLPLLARNVTLHGFQNGGETEFLVLLRRYVDFAR
                     ELRQLAGADGMIHVENCDDAERLVDVLGYQFAEGCGEKNGYVVTANAERAFLAIDSGF
                     PLTALEEALQKHQPYEYAFPATPVPVIFHERDWTGVTVWHQKSGTNLLDALLHDRQLD
                     TLYWALSKNDEGTRSALRERRGLRWML"
     gene            1486..>2125
                     /locus_tag="VHE33_20320"
     CDS             1486..>2125
                     /locus_tag="VHE33_20320"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HVT99856.1"
                     /translation="MLARPVRPGAPRLAPSLALLLTLLLASVSLTGCGSISGNLGDLT
                     AISASSASVRVNQQLQIQSRMNVTAVPLTFFINGVQGGNAQLGTIDAAGLYTAPAQVP
                     TPNTVTVTATANGHPEAAPGSVTVAVLNPIPVVTGVLPSGITEGVTTITVTGSQFVYG
                     AQILWNGAPVATTYVSSTQVAAAITAPAPGTFPLLVSNPNPGSANSGTLNVPV"
BASE COUNT          376 a          784 c          548 g          417 t
ORIGIN      
        1 cagcatccac cgcaggccgc gccgctctcg cagagccgag cgtgtcccct catcgttctt
       61 cgacagcgcc cagtacagcg tatccagctg gcggtcgtgc agcagcgcgt ccagcagatt
      121 cgtcccgctc ttctggtgcc acaccgtgac tccggtccag tcccgttcat gaaaaatcac
      181 cggtaccggt gtcgccggaa aagcgtattc gtacggctgg tgtttctgca gagcttcctc
      241 aagcgccgtc agcggaaagc ccgaatcgat ggccagaaac gcgcgctctg cgttggccgt
      301 cactacataa ccgttcttct cgccgcaccc ttctgcaaac tggtatccca gaacatccac
      361 cagccgctcc gcatcgtcgc agttctccac gtggatcatg ccgtcggcgc ccgccagttg
      421 ccgcagctcc cgcgcaaaat ccacgtagcg gcgcagcaga accagaaact ccgtttcgcc
      481 gccgttctga aatccgtgca gcgtaacatt gcgcgccagc agcggcagca catcttccgg
      541 ctccgcttcc tggctgatcc ccgccatgcg caggaacgag cgcaacgggc ccgggattgg
      601 aatcgactcc cctccccccg gcgcagccgg cggtttgacc gtgtccgacg gtcgtgccgc
      661 cttcgccgtc aggcaaatac tgaagaaaat gcagcagccc agcaccgtag ctgaaaggcg
      721 aagacgacgt ctcatgaccg aagaccctcc gattacccag gacaggcgaa gtgcaatcgc
      781 gcacaaacgt aggggagaca cgactcattg ccgccggagg cgaaccgcgt gggctttgcc
      841 tgtgcggtct ttcgagacga ctccccatct tccgaatcgg tcacgtgcgc ggaagttcgg
      901 ccagctttgg agcttcgtcg cttgaacggt ttcgccgtcc gtcagccacg cgcttcggat
      961 tccttgttcg atcccccgcg tgtgggcacc cattgtcgca aatcttttgc ggttctggcg
     1021 aattcatttc tcgtgcagtg aaaatagtgc gggtattcgt tctctgagtc gtttccgaat
     1081 gtcacgcaat tgtctccact gcacctcgcg tatcccgtcc ccccgccgct gaggcgcact
     1141 gctggcgtga gatggcgtgc cagtcccccg gaacacgatg gctggtttct gtgcaggcgt
     1201 ctccggcccc gcctgaaaac gtcccgcccc gtagagcccg caggctggtg acttcgcccc
     1261 tctcctgaca tcagtaacgc tcctcatcca cgcgggccgc gctgcacaaa aggtaaaaat
     1321 cctacccagg gcgatccgcc gcggcacaga ccactttcgt ggtacttctg ccggttcttt
     1381 tcacacaacg gttcgcactt tccggtgata cgctgtgcca acaaactcac ccgccaggct
     1441 caccggccaa tctcgaagcg cagctttcat cccccaggag gatgcgtgct agcaagaccc
     1501 gtacgtccag gtgcccctcg tctcgcaccg tctctggctc tgctcttaac tctgctgctg
     1561 gcatcggttt ccctcaccgg ctgcggctcc atcagcggca atctcggcga tctcaccgcc
     1621 atctccgcct cctccgcctc ggtccgcgtc aatcagcagc tccagatcca gagccgcatg
     1681 aacgtgacgg cagtgccgct caccttcttc atcaatggcg ttcagggcgg caatgcccag
     1741 ctcggcacca tcgatgccgc cggcctctac accgcccccg ctcaggtccc cacgcccaac
     1801 accgtcaccg tgaccgccac cgcgaatggc catcctgaag ccgctcccgg cagtgtgact
     1861 gtcgcagtcc tgaatccaat tcccgtcgtc accggcgttc tgccctccgg catcaccgaa
     1921 ggcgtcacca ccatcaccgt caccggttcg cagttcgtct acggtgcgca gattctctgg
     1981 aacggcgctc ccgtcgccac gacgtatgtc tcctcgacgc aagtcgccgc cgccatcacc
     2041 gcgcccgctc ctggaacctt tcccctgctg gtcagcaacc ctaaccccgg ctccgccaat
     2101 tccggaaccc tcaacgtccc cgtcg
//
LOCUS       DASZYN010000808         2823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_313988, whole genome shotgun sequence.
ACCESSION   DASZYN010000808 DASZYN010000000
VERSION     DASZYN010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313988"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1078..2307
                     /locus_tag="VHE33_20325"
     CDS             1078..2307
                     /locus_tag="VHE33_20325"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HVT99857.1"
                     /translation="MKRVFLYLLAALVAPSLLFAASTSTAHRTAKHRSRVHESTAARR
                     HLANLQHSHRAPRAHLATASLTTHSRHRRHRFYERFTASSFAASDVTAGDITAGEDPI
                     VRQAAIDALGGMNGTVVAIDPSNGRVLAMVNQKLALSSGAEPCSTIKLSVALAALEEG
                     IVTKDTPVNLGGRYHLNLTEALAHSNNAYFETLGRRLGFDRVRHYATQFGLGELAGYN
                     IAGEQLGTYPDQPIAEKLGGVGRMCSFGEGVSMTPLQLGALVAAIANGGTLYYLQHPT
                     TPAEALTLQPRIKRTLDIGPILPEIQDGMAAAVRYGTARSLRANFNQFPVLGKTGTCS
                     NDGTRFGWFASYADTQYGRIVAVFFLEGGRPTFGPKAAELTGVFYRNLWDSSYFAAKQ
                     APETPDSPATTAVGAPQ"
BASE COUNT          602 a         1046 c          713 g          462 t
ORIGIN      
        1 ggggcgttct gatctcggtc ccacgccgct cagcggttat cggcgtggtc caccaccgcg
       61 ggagccctgc gcggtcgcgc aaatgacgac tcccaaaacg taacgcacac accgaggaag
      121 acgacaacgc agcaagacga aacacgggaa gacgaaacgc aggaaggcga aacgcagcaa
      181 gaagcgaaca agccaccagg cgaaggagtt aaaccgggcg cactcccctt ttgggaaagc
      241 accaggttca ccccacagca ggaagcgaac aagcgccacc aggcgaagga gttaacaccg
      301 ggcgccccct ttgggggaag cgccaggttc accccaaagc aggaagcgaa caagcgccac
      361 caggcgaagg agttaacacc gggcgttccc ccttggggga agcgccaggt tcaccccaca
      421 gcaagcagcg gacgccttga gagcacacgg agtcaaccca gttcacgccc ccggccaatc
      481 cgaagtcacg ctgccggacc atttcgggaa acatccacca ccgagcgcac tccccttttg
      541 ggaaagcgcc aggctcaccc cacagcaagc agcgaacgcc ttccgcgtga gaaagtaaac
      601 ggaattgatc ctgttcccca cccgaaagta ctgtgaccgg aatccgctga accagtctgg
      661 gaaagcaggc ctctccaccc gctccccagc actcctcgat acgccggccc ggcacggtcc
      721 cgcaggccac agccccaata cctcttggta ctcagtcctc accggaaaga gccatgctcg
      781 catacgaaaa tgtactggac agacaatagt ttccggttat attgaaccaa tagttcctgc
      841 catctgcccg ggccctgaca cgttggatac ttccttccaa agcaactaca ggggtttctt
      901 cgggttcagg tcgggcatgg cgagaacaga ctttccgcac atgggactca agttccagtt
      961 cgggaaggcc gatgagaatc ggttactttg caccgtacga agtaaggttt cgccagacag
     1021 gtttctgcgg catcatcagc cagagggtct ggattcttcg aacgagagta tctcttgatg
     1081 aagcgcgttt ttctgtatct gctggctgcc ctggtcgccc cgtcgctcct cttcgcggcg
     1141 tccacctcca ctgcgcatcg caccgccaaa caccgctcgc gcgtccatga gtcaacggcg
     1201 gcgcgtcgcc acctggccaa cctgcagcac agccatcgcg cccccagggc ccatttggcc
     1261 acagccagcc tcacgactca ttccaggcat cgccgccatc gcttttacga gcgcttcacc
     1321 gccagttcct ttgccgcttc cgacgtcacc gccggcgaca tcaccgcggg tgaagatccc
     1381 attgtccgcc aggctgccat cgacgccctc ggcggtatga acggaaccgt cgtcgccatc
     1441 gatccctcca acggccgcgt cctggccatg gtcaaccaga agctggcctt gtccagcggc
     1501 gccgagccct gctccaccat taagctttct gtcgccctgg ctgccctcga agaaggcatc
     1561 gtcacaaagg acactcccgt caatctcggc ggccgctacc atctcaacct caccgaggct
     1621 ctggctcact ccaacaacgc ctacttcgaa acgctcggcc gcaggctcgg ttttgatcgc
     1681 gtccgccact acgccactca attcggcctc ggcgagttgg ctggctataa catcgctggc
     1741 gaacagctcg gcacctaccc ggatcagccc atcgctgaga agctcggcgg agtcggccgc
     1801 atgtgctcct tcggcgaagg cgtctccatg acgccccttc agctcggagc cctcgtcgca
     1861 gccatcgcca acggcggcac cctctactat ctgcagcacc cgacgacccc tgccgaagcc
     1921 ctcaccctgc agccgcgcat caagcgcact ctcgacatcg gccccatcct ccctgaaatt
     1981 caggacggaa tggccgccgc cgtccgctac ggcacagcgc gttcgctccg cgcaaacttt
     2041 aaccagttcc cggtgctggg caagaccggt acctgctcca acgacggaac tcgcttcggg
     2101 tggttcgctt cctacgccga cacccagtac gggcggattg tcgctgtctt cttcctcgag
     2161 ggcggccgtc ccaccttcgg ccccaaagcc gccgagctta ccggcgtctt ctaccgcaat
     2221 ctctgggact ccagctactt cgccgccaaa caggctcctg agacgccgga ttcacctgcc
     2281 acaacggcag tcggcgctcc tcagtaggtc catccgcgct gcgaacctgc tgccccagcg
     2341 gcacaccgag gcaaatcact cgcccgcgta ccgcgcgtaa ggcaggttcg ccccacagcg
     2401 gcaccgcgcg ccttcgccat ccaaatttca acaaaaacaa agatgtccct ccgccggagg
     2461 tgccgtcctc gatcgtccca gtgccaattg aggagccgcc ccaccccaca acaaagttcc
     2521 gcgcaccagg tgccagacac cagcccgatt ttccaatccc cgaaaacctg tggggagcca
     2581 ggttcacccc acagcaagca gcggacgccg tcagaacccg ggcagtcaac ccgattcact
     2641 cccccgccca atccgaagtc accatcgccg gaccatttcg ggaacgccgc ccccgacacc
     2701 cacccctcac accccgcgca agcccaggtt caccccacag caagcagcaa acgccttccc
     2761 accccaggat tcaacagcat tcccaccccg ctcaacccat ccgtacccag gtctgtgcca
     2821 ttc
//
LOCUS       DASZYN010000809         5495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_57267, whole genome shotgun sequence.
ACCESSION   DASZYN010000809 DASZYN010000000
VERSION     DASZYN010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57267"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /gene="ggt"
                     /locus_tag="VHE33_20330"
     CDS             complement(<1..1526)
                     /gene="ggt"
                     /locus_tag="VHE33_20330"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HVT99858.1"
                     /translation="MRIRSRLVLSLCLLAVSPLGAQDRSYGRSVVSTPYGIVATSWVQ
                     ASQAGALMLDKGGSAIDAAIAANAVLNTSEPMMNGMGGDLFAVYWDAKTGKLYGLNAS
                     GWSPQALTIEHLRAKGMTTMPSKGIDSVTVPGMVDGWTKLHSRWGKLPWRELFEPAIF
                     YADHGVTVPEMIHDYWTASASQLSENGDSRRVFLPEGKVPAAGQIFMNPDLANTLRVI
                     AAQGEDAFYKGPIAQAILKTSSENGGTMTADDLAQYSAEWVEPISTTYRDWTVYELPP
                     NGDGMAALEMLNIMEQSAADPAGPRSPGELHTRIEAMKLAYADVKAYDGDPRFSKIPV
                     TQLLAKSYAAQRAKLIDSAHANCTVAPGALPGSDTTYLSVVDHDGNIVSFIQSNYAEF
                     GSGITVDGMGFVLQDRGGLFNLDPKSPDALAGHKRPLHTIIPGFMQRGDEHIGFGIMG
                     GWNQPLAHAQFVSNIADYHMNIQAALSEPRFTVSSKLGCTIVIESRVGQATIDQLTKM
                     G"
     gene            1792..2040
                     /gene="acpP"
                     /locus_tag="VHE33_20335"
     CDS             1792..2040
                     /gene="acpP"
                     /locus_tag="VHE33_20335"
                     /inference="COORDINATES: protein motif:HMM:NF002149.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HVT99859.1"
                     /translation="MASGVEEKVKQIIVEQLQVDEAEVTPTASFQEDLGADSLDVVEL
                     VMQFEEAFDLEIPDEDAEKIKTVKDAIDYIDKNAKGGK"
     gene            2043..3287
                     /gene="fabF"
                     /locus_tag="VHE33_20340"
     CDS             2043..3287
                     /gene="fabF"
                     /locus_tag="VHE33_20340"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF004970.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HVT99860.1"
                     /translation="MQRRVVVTGLGLVSAVGNTAPEVWNNLLAAKSGIATITHFDTTN
                     FPVTFAAEVKNFDPLNFVDKKEARKMGRFIHLALAATHEAMETSGLKVTPEIGDRVGV
                     FIGSGIGGFDVIEREHNNMLQGGPRKISPFFIPAAIINLAAGQVSIRYGAKGPNEATA
                     TACTTSAHAIGDAYRIIERGDADVMIAGGSEAAITPMGVGGFAAMRALSTRNDDPQHA
                     CRPFDRDRDGFVVGEGSGILVLEELEHAKARGAKILAEIIGYGMSADAYHITGMPEDG
                     DGCYRAMEHALKSAKIQPNDVDYVNAHATSTTLGDVVESKAMQRLFGERATSKKLMIS
                     STKSMTGHLLGGAGGLEAGITILALMNQTAPPTANLDHPDPECVLNYVPNTPQKASMH
                     VALSNSFGFGGTNGSLIFRRWE"
     gene            3700..5274
                     /locus_tag="VHE33_20345"
     CDS             3700..5274
                     /locus_tag="VHE33_20345"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HVT99861.1"
                     /translation="MSVLLINPSDNSFGTAVITPRWLFVLAAATPRSVGDPILIDESL
                     EQIVPERIQPGDIVGISVHTGNALRGYHVGRMARERGAWVVYGGIHATLFPEEALERG
                     QAHAVVKGDGDLVWAKVVKDCLAGNPDKVYEGGRIAGSDFLAARWDLMRPEKYMWASV
                     QTIRGCPKHCSFCSVWRTDGQKPRQRRHQSVIDEIVNLRRLGFRFVALADDNFYPVTL
                     TDLRLAREQNNQAKLAELTEIREERFALMEELARLPSDMVFFTQITMEAGEDGDYLDA
                     MRKANIKGALVGVEAVTPEGLKAVYKDWNYSGEALAKQLQTFKAHGVHVLGSFIFGLP
                     TDKPATFGATVEMAMKAGVTFAQFVMMTPFPGTVDFGRWEKTQSVDPAMVGDVPITRY
                     WLIPTEVRPKMFTPHPTMSSDEIRDRTQKVWDRFYNWPAIWERSACCPNVTSRIAFIF
                     LSKLYRQMYAGTGISSDSARRRKSKRWARWTARQCKRLFRATPMPELQSPAWDMPVWA
                     RAQRPELLSIRNRADS"
BASE COUNT         1102 a         1805 c         1620 g          968 t
ORIGIN      
        1 cccatcttcg tcagctgatc gatcgtcgcc tgccccacgc gcgactcaat cacgatggtg
       61 caacccagct tgctgctgac cgtaaaccgg ggctcagaaa gcgccgcctg gatgttcatg
      121 tggtaatcgg caatgttcga aacgaactgc gcgtgcgcca gcggctggtt ccatccgccc
      181 atgatcccga acccgatatg ctcgtccccc cgctgcataa accctgggat gatcgtgtgc
      241 agggggcgct tgtgcccggc cagcgcgtcc ggcgacttcg ggtcaaggtt aaagagcccg
      301 ccgcgatcct gcagaacgaa acccatccca tccaccgtga tcccggaacc aaactccgca
      361 tagttgctct gaatgaacga cacgatgttg ccgtcatggt cgacaaccga caaataggtc
      421 gtgtcgctgc cgggcagcgc ccctggcgca accgtgcagt tagcgtgcgc ggaatcgatc
      481 agcttcgcgc gctgtgcggc atacgacttt gccagtagct gggtgacagg aatcttcgag
      541 aagcgcggat cgccgtcata cgccttcacg tccgcatacg ccagcttcat cgcctcaatc
      601 cgcgtgtgca gctcgcccgg gctccgtggt ccggcagggt cagccgcact ttgctccatg
      661 atattgagca tctccagcgc cgccatgccg tcgccgttcg gcggcagctc gtagaccgtc
      721 cagtcccgat acgtcgtcga aatcggctcc acccactccg ccgaatattg cgccagatcg
      781 tccgccgtca tcgtgccgcc attttccgac gaggttttca gaatcgcctg cgcgatcggt
      841 cccttgtaaa aggcatcctc gccctgcgct gcgatgaccc gcagcgtatt cgcaaggtcg
      901 gggttcataa agatttgtcc ggcggcagga accttccctt ccggcagaaa aacgcgccgg
      961 ctgtcgccgt tctctgagag ctgcgaggcc gaggccgtcc agtagtcgtg gatcatctcc
     1021 ggcaccgtca cgccgtggtc cgcatagaaa atggccggct caaacagctc acgccacggc
     1081 agcttccccc agcgggagtg cagcttcgtc cacccgtcca ccatgcccgg aaccgtgacg
     1141 gagtcgatgc ctttggaggg catcgtcgtc atccctttgg cccgcaggtg ctcaatcgtc
     1201 agcgcctggg gcgaccaccc gctggcgttc agcccataaa gcttccccgt tttcgcgtcc
     1261 cagtagactg caaagagatc gccgcccatc ccgttcatca tcggttcgga ggtattcagc
     1321 accgcgttgg cagcgatggc cgcgtcaatc gccgatccgc ccttatcgag catcagggcg
     1381 ccggcctgcg acgcctgcac ccagctggtg gccacgatcc cgtacggagt cgagacaacg
     1441 gagcgcccgt acgagcgatc ctgcgcgcca agcggcgaga cggccagaag gcatagcgac
     1501 aagacgaggc gggagcgaat tctcatgggc gaccttttcc gttgatgtgt ccgggacggc
     1561 ggcgccccgg gcgacaccag ggtagcagat tgccggaaga gagtcctttc ctgcgatcgt
     1621 gcatcatcat gggggagtcg gctttacact accagggtgc ctcagtagac ttggaatttc
     1681 ggagctgagg ggaggcacgc tgcgagccaa cctcagtgtg ctcgcggagg cttcacatcc
     1741 tccctcccaa ctttcagcat caacaggaac gaaggaccac aggagagacg catggcatca
     1801 ggtgtggaag agaaggtcaa acagatcatc gtcgagcaac tgcaggtgga tgaggcggaa
     1861 gtgacgccga ccgcgtcctt ccaggaagac ctgggcgccg attccctcga cgtggtcgag
     1921 ctggtgatgc agtttgagga agccttcgac ctcgagattc ccgacgagga cgccgagaag
     1981 atcaagaccg tcaaggacgc gatcgactat atcgacaaga acgcgaaagg cggcaagtaa
     2041 tcttgcaacg gcgtgtcgtc gtcacgggtc ttgggctggt gtcggcagta ggcaacacgg
     2101 cgcccgaggt ctggaacaac ctgctggctg cgaagagcgg gatcgccacg atcacgcact
     2161 tcgacacgac gaactttccg gtcacctttg ccgccgaagt caagaacttc gaccccctca
     2221 actttgtgga caagaaggaa gcgcgcaaga tggggcgctt cattcatctg gctctcgccg
     2281 cgacccacga agccatggag acttccggcc tgaaggtgac gccggagatt ggcgaccgcg
     2341 tcggtgtgtt catcggatcg ggcatcggcg ggttcgacgt catcgagcgc gaacacaaca
     2401 acatgctgca gggtggcccg cgcaaaatat ccccattctt tattcccgcg gccatcatta
     2461 atttggcggc aggccaggtg agcatccgct acggagcaaa ggggccaaac gaggccaccg
     2521 ctaccgcctg caccaccagc gcccacgcca ttggtgacgc ctatcgcatc atcgagcgcg
     2581 gcgatgcgga cgtcatgatc gccggcggct ccgaagccgc cattaccccc atgggcgtcg
     2641 gcggattcgc agccatgcgt gcgctctcca cccgcaatga cgatccccag cacgcctgcc
     2701 gccccttcga cagggaccgc gacggattcg tcgtcggcga aggttcgggc atcctcgtcc
     2761 tcgaagaact cgagcacgcg aaggcccgcg gcgcgaagat cctcgccgag atcatcggct
     2821 acggcatgag cgcggatgcg tatcacatca ccggcatgcc ggaggacggc gacggctgct
     2881 atcgcgcgat ggagcacgcg ctcaagtcgg cgaagatcca gcccaacgat gtcgactatg
     2941 tgaacgcgca cgctacgtcc acgaccctgg gcgatgtcgt cgagtccaaa gcaatgcagc
     3001 ggctcttcgg cgaacgcgcg acgagcaaaa aattaatgat cagctccacg aagtcgatga
     3061 ccggccacct gctcggtggc gccggcggcc tcgaagcagg aatcaccatc ctcgccctga
     3121 tgaaccagac agctcctcca accgccaacc tcgaccatcc cgaccccgag tgcgtgctga
     3181 actacgtgcc caacacgccg caaaaggcgt ccatgcacgt cgccctgtcc aactccttcg
     3241 gcttcggcgg aaccaacggc tcgctgatct tccgccgctg ggaataacgc agaaatttcg
     3301 gatccccgtg ctgccggctg tgcctcacgg tcacagccgg cggcggagcg gatcgagatg
     3361 cccttcgccc ctgggcgatt cctccttctc aacgcggctt tcccgcgcaa ccacgaccgg
     3421 cgctaactta cgcggaaccg gccggcagcc ctgaaacctg cggacgacca aggtctcctg
     3481 ttgccttcgc atgtgaagta agccgggatc cccgcctttg cccggagcca tattttcgta
     3541 actgttacga atcggcgact ccaatggcgt gacttggcgc gtatgatcgc agacagtctg
     3601 gccgacggcc tcaagccaga ctctggatcc gaatccgaca agctttctcc acaatttgca
     3661 ggacgctctc tgaggccccg cactgaaagg ttggtaccta tgagcgtgct cctgatcaat
     3721 cccagcgaca attcctttgg caccgccgtc atcacacccc gatggctgtt cgtcctcgcg
     3781 gctgcgactc cccgttccgt cggagatccg atcctgatcg acgagtcgct cgagcagatt
     3841 gtgccggagc gcatccagcc cggcgatatc gtcggcatca gcgtccatac cggcaatgcg
     3901 ctccgcggct atcacgtagg gcgcatggcc cgcgaacggg gcgcatgggt tgtgtacggc
     3961 ggtattcacg ccacgctttt cccagaagaa gccctggaac gtggccaggc gcacgccgtg
     4021 gtcaagggcg atggcgacct cgtgtgggcg aaggtcgtga aggattgcct cgccggcaat
     4081 ccggacaagg tttacgaggg cggacgcatc gccggcagcg acttcctcgc cgcccgctgg
     4141 gatctcatgc gcccggaaaa atacatgtgg gcctcggtcc agaccattcg cgggtgcccc
     4201 aaacattgct ccttctgcag cgtctggaga accgatggcc aaaagccgcg gcagcgccgg
     4261 catcagagcg tcatcgatga aatcgtcaac ctgcgccggc tcggctttag attcgtcgcc
     4321 ctggctgacg ataattttta tcccgtcacc ctcaccgatc tgcgcctggc ccgcgagcaa
     4381 aataatcagg ccaaactggc ggagctgacc gagatccgcg aggagcgctt tgcgctcatg
     4441 gaagagctgg ccaggctgcc cagcgacatg gtcttcttca cccagatcac catggaagcc
     4501 ggcgaggacg gcgactacct cgacgccatg cgcaaagcca acatcaaagg cgcgctcgtc
     4561 ggcgtcgagg ccgtgacccc ggaaggcctc aaggccgtct acaaggactg gaactactcc
     4621 ggcgaggccc tggcgaagca actccagacc ttcaaagcac acggtgtaca cgtgctgggg
     4681 tcgttcatct tcggcctgcc caccgacaag cccgcgacct tcggcgcgac cgtggaaatg
     4741 gccatgaaag cgggcgtcac gttcgcgcag ttcgtcatga tgaccccctt cccaggcacg
     4801 gtcgattttg gccgctggga gaagacgcaa tcggtggatc ccgccatggt gggcgacgtg
     4861 cccatcaccc gctactggct catccccacc gaagtgcgcc ccaagatgtt tacaccccat
     4921 cccaccatga gttcggatga gattcgtgac cgaacccaga aagtgtggga ccggttctat
     4981 aactggccgg cgatctggga gcggtcggcc tgctgcccca acgtgacctc gcgcatcgcg
     5041 ttcatcttcc tttcaaagct ctaccggcag atgtatgcgg gcacgggcat ttccagcgac
     5101 agcgcgcggc gcaggaagtc caaacgctgg gcgcgctgga ccgcccgcca atgcaagagg
     5161 ctctttcgag ccacgccgat gccggaactg cagtcgccgg cctgggatat gcccgtttgg
     5221 gcccgtgccc agcgcccgga gctgctcagc atccggaaca gggccgattc ttaagttcag
     5281 cctggaagcg cgacccacgg agcgctgctt accgcgaaat gattcaggaa gggtgtggcc
     5341 tcgcacggcc cgcgatcacg ctaaaacgag atcgacggca gccacgcgcc tgcaggatcg
     5401 ttgtagagac cgaagctcat cagcgtcatc ggctgaacaa tgcgcagccc ctcggaaaga
     5461 caccagcgca gaagactggc gttgcgcgac ggaac
//
LOCUS       DASZYN010000810         8328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1154841, whole genome shotgun sequence.
ACCESSION   DASZYN010000810 DASZYN010000000
VERSION     DASZYN010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8328
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154841"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1438)
                     /locus_tag="VHE33_20350"
     CDS             complement(164..1438)
                     /locus_tag="VHE33_20350"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HVT99862.1"
                     /translation="MNRCTRLGIINEASALVAALAIAVSLFGQKQSALQSVPPSITAQ
                     EQKGIDRDISRHFGDAPADPGLKAKLSGAMRAADVRAAMRKVADWELAWSQPYFDRIW
                     TWSVLYSGFMAASPALDDPKYRDAMQAMAEKFQWDLRSQHPNADDQSVGQTYLELDLL
                     QPAPEKSRPTQAALDELIAGGAARIPEGQAQIPWWWCDALFMAPPVWSRMYAVTHDPR
                     YLDYLDKHWWETSHLLYDAQRHLYYRDITFLHQTDDHGNPLFWSRGNGWVMGGIARTL
                     DYMPENYPSRRKYEAQLREMAAAVAALQDKQTGLWHSDLLDPLDHPQPEVSGSALITF
                     ALAWGVNHGVLDRAAYKPIIAKAWRGLVGQIYEDGRLGNIQQTGAAPAHYLPSSSYNY
                     GVGAFLMAGAQVEEFVSHRGHAKRAASTQGGR"
     gene            complement(1435..4218)
                     /locus_tag="VHE33_20355"
     CDS             complement(1435..4218)
                     /locus_tag="VHE33_20355"
                     /inference="COORDINATES: protein motif:HMM:NF028442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HVT99863.1"
                     /translation="MIAVRKLMPCGAALCLTAASLVGAQQNSTTTRRPTNAGDSLASL
                     RRGFAAPPMEARPMMRWWWFGPAVEPGELARELRTMKAGGIGGVEIQPVYPLQLDDPQ
                     KGFRNLKFLSPEFLKMVTFAANTGHELGMRVSITLGSGWPYGGAWVPVTESAGRLRVV
                     AVAVPAGLHSVAVPSLENGETLLASFVAAGTSERYDADHARRVEDVRDGRLQLPSDLG
                     DPRVALFFISSRTGQQVKRPAYGAEGFVIDHFSAGAVQDHLRNVADPIVGAFGDHPPY
                     SVFSDSLEVYESDWTADFLSEFRNRRGYDLTPYLPELVAGTGPEAADLRYDWGRTLAE
                     LIDQNYLGAINAWAHAHHTEFRSQTYGSPAVTLSSNSRVDLPEGEGSQWHGPFSFTRW
                     ATSASHVYGRPVTSSETWTWLHSPAFSATPLDMKAEADTFFLQGINQLVGHGWPYSPA
                     SAGEPGYSFYAAAVFNDHNPWWIVMPDVTAYLTRMSWLLRQGQPANDVAVLLPEEDAE
                     AHFRLGEASVSGQMAKLLGTDLVPAILDAGYNFDFIDSEAIDRAGVHYPVLVIPDARS
                     IPLATYRKIAEYVKNGGMVLATGDSPTRAPGLVESPRDTPRVLQISKQLFAPDAQNAK
                     LVKRATDLGPTIGAFLKPDVALEPATPEVGFIHRKLADADIYFLANTDNRPHVFSATF
                     RTERPAVSLWDPFSGKISDAGEGPKLSMTLAPYASQVVVFTDRAVGQKGTDTATNFSP
                     IDLDHDWKVTLDKTGFSEAMHSLHSWSDDEAGRYYSGTASYTRTVDLPADFPKGATAM
                     LDFGAGSPVRRRGLNFPGMRTWLDAPLRDAAVVYVDGKRAGSVWHPPFVLDIGPFLHA
                     GSNELKIVVANTAINELAGRTPTDYRLLWMRYGKRFAPQDMDHLEALPSGILGPLRLV
                     PAGETSGSGEAAQ"
     gene            complement(4287..5576)
                     /locus_tag="VHE33_20360"
     CDS             complement(4287..5576)
                     /locus_tag="VHE33_20360"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HVT99864.1"
                     /translation="MRIRFGFLPVLSGLVVLGLAAGSRSWAAAVSTVCDPHAYGAKAD
                     GIARDTTAIQRAVDACEQRGGGTVRLSAGTWLSAPIMLKSKITLQLDKGATLLGSPDH
                     DDYPSITEFKAPGRRSLVSATNATNVSITGEGTIDGNGQSWWDEARAVKDHGVMGSDH
                     TRPRLVVFDHCRHVLVEGVTIQNSPMWQLVPYYSDDVTIRNIRVLAPQHSPNTDAIDP
                     FSSSHVRIDHVFADVGDDDVAIKSGIANSPGPDDPSRDITITDCNFLHGHGLSIGSEV
                     AGGAQNVRAERIHFDGTDNGIRVKANRDRGNDVGNFVFRDIDMKNVKNAIIISEYYPK
                     ILPPDPDPAQPVTRLTPHFHDITLENVTATDIVSAGAIAGLPEAPIRNVVLKDVHIQA
                     QHGMAISNADVSGQRVSVEAADGSGIIKAAGGNVNLH"
     gene            5709..7355
                     /locus_tag="VHE33_20365"
     CDS             5709..7355
                     /locus_tag="VHE33_20365"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HVT99865.1"
                     /translation="MNSFRSNRREMLRMSALGLGAVVGSSLPEAYALPRSPAPSQGMP
                     GIFDVRTYGAVGDGKTVDTPAINKAIEAAAAAGGGTVVFPPGTWLSFSIHLKSHVGLY
                     LAQSATLLAADSPLPGQTTGYNGGTYDAAEPNTPWEDYQDYGHNHWHNSLIWGENLSD
                     ISITGPGTIYGKGLSFGARRNARGNYPIYVAEQAGVGNKSIALKNCHNVLLRDFSILK
                     GGHFGLLLTGVDNLTIDNLTIDTDRDGIDIDCCRNVHVSNCTVNSPWDDGICPKSSYA
                     LGYARATENVTITNCYVTGDYELGTVLDGTFKRFAADAHVGRTGRIKCGTESNGGFKN
                     ITISNCVFDTCNGLALESEDGALCEDIAVTNITMRNITTSPIFVRLGARLRGPKETTK
                     VGTMRRILISNLTSYNCASRLCSILSGIPDYAIEDVKLANIYQHHVGGASQDTLAIQP
                     PENENKYPDPGMFGPMPSQGFFLRHIKNLEMSHVEVAAATSDPRPSFYLAGVDRADFI
                     AVTAPKGVQAFHLGKVTDLRILLSRAAKDTQLDTADNQTI"
     gene            7778..>8328
                     /locus_tag="VHE33_20370"
     CDS             7778..>8328
                     /locus_tag="VHE33_20370"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVT99866.1"
                     /translation="MTQTNRRAPVGGRSLLWALALCLMTAPAFAQLAGTGTIQGTISD
                     PSGAVVPGAEVTVTGTATSAKHQTVSSGGGVFSFPNLPIGTYNLSVTASGFKAYTQSG
                     IVLEVGSNIGVNVNLSVGAANQEVHVQANGIALQTQDTSFKQTIDQKEVNELPLNGRQ
                     MTDLIVLSGGAASAPGNDMVGSK"
BASE COUNT         1740 a         2617 c         2365 g         1606 t
ORIGIN      
        1 aggtcggcca cgcggatcgc cacgtgcgcg atgccggtga gattgggtgt cgccggtgga
       61 gcggaatccg gagtctgtcc gccggcgtgg aggcagcaac tgaacacaat cgcaactgcg
      121 gcgcgaaaaa cgccgcgtgc atgttcgaaa ttggcgtatc gcttcatcgt cccccctgcg
      181 tgctcgcagc cctcttcgcg tggccgcgat gggatacaaa ttcctccacc tgagctcctg
      241 ccatcaggaa tgcacccacg ccgtagttgt agctcgaaga gggcaggtaa tgcgcaggcg
      301 ctgcgcccgt ttgttgaatg ttgcccagac gtccatcctc atagatctga ccgacgagcc
      361 cgcgccaggc tttcgcgatg atgggcttat aagctgcccg gtcgagcaca ccatgattca
      421 cgccccatgc gagcgcgaac gtgatgagcg cggagccgga aacttccggt tgcgggtggt
      481 cgagcggatc gagcagatcg gagtgccaga gtcctgtctg cttgtcctga agtgcggcga
      541 ccgctgcagc catctcccgc agctgcgctt catatttcct gcgcgaggga tagttttccg
      601 gcatgtaatc cagcgtgcgc gcgatgcccc ccatcaccca gccgttgccg cgcgaccaga
      661 acagcgggtt gccatggtcg tcggtctgat gcaggaaggt gatgtcgcgg taataaaggt
      721 ggcgttgcgc atcgtagagc aagtgcgacg tctcccacca gtgcttgtcc aggtaatcga
      781 gatatcgggg atcgtgcgtc accgcgtaca tgcgtgacca tacgggcggc gccatgaaca
      841 acgcgtcgca ccaccaccac ggaatctgcg cctgcccctc gggaatccgc gccgcgccgc
      901 ccgcgatcaa ttcgtcgagc gcggcctggg ttggcctcga cttttccggt gcaggctgga
      961 gcaggtccag ttccaggtaa gtctgcccta cgctttgatc gtccgcgttc ggatgctggg
     1021 accgcaggtc ccactggaac ttctctgcca tcgcctgcat ggcgtcgcga tacttcgggt
     1081 catcgagtgc aggcgaggcc gccatgaaac cgctgtaaag aacgctccac gtccagatcc
     1141 ggtcgaagta aggctgcgac caggcaagtt cccaatcggc aactttgcgc atcgcagcgc
     1201 gcacatcggc ggccctcatt gcgccggaga gcttcgcttt caaaccggga tccgccggag
     1261 catcgccgaa gtggcgcgag atgtcgcggt cgattccctt ctgctcctgt gcggtgatcg
     1321 acggcggaac ggattgcagg gcggactgct tttgcccgaa cagtgacact gcgatcgcta
     1381 acgctgcgac aagagcagag gcttcgttga tgattcccaa ccgtgtgcag cggttcattg
     1441 cgcagcctct cccgaaccag atgtttcgcc cgccggcacg aggcgcagcg ggccaaggat
     1501 gcccgacggc agcgcctcca gatgatccat atcctgaggt gcaaaccgct tgccgtagcg
     1561 catccatagc agccgatagt cggtgggcgt gcggccagcc agttcattga tggccgtgtt
     1621 cgccaccacg atcttcaatt cattcgaacc ggcatgcagg aaaggcccga tatccagaac
     1681 aaacggcgga tgccataccg aacccgcacg cttgccatcc acgtacacca cggccgcgtc
     1741 gcgcagtggc gcgtccagcc aggtgcgcat tcccggaaag ttcaagcctc tccgcctcac
     1801 cggcgagccg gcgccgaaat caagcatcgc cgtcgcgccc tttgggaagt cggccggaag
     1861 atcgactgtc cgcgtgtagc tcgccgtgcc agagtagtat ctgcccgcct catcgtcaga
     1921 ccatgaatgg agcgaatgca tcgcttcgct gaagccggtc ttgtccagag tgaccttcca
     1981 gtcatggtcg agatcgatcg gagaaaagtt tgttgccgtg tctgtacctt tttgcccgac
     2041 ggcgcgatcc gtgaaaacca ccacctggga cgcatacgga gcgagagtca ttgagagctt
     2101 cggcccttcg ccggcgtctg agattttccc cgagaacgga tcccagaggg agaccgccgg
     2161 ccgctcggtg cgaaaagtcg cactgaagac atgaggccga ttgtcagtgt tggccaggaa
     2221 atagatatcg gcgtccgcca gcttgcgatg aatgaagccg acctcaggtg tggccggctc
     2281 gagcgccaca tctggcttaa gaaaggcgcc aatggttggc cccagatcgg ttgccctttt
     2341 caccagcttc gcgttttggg catccggcgc gaacaattgc ttcgaaatct gaagaactcg
     2401 aggcgtatcg cgaggactct cgacaagccc cggcgcccgc gtcggagagt cccccgttgc
     2461 cagaaccatg cccccattct tcacatactc cgcaatcttc cggtaggtgg cgagtggaat
     2521 gctgcgagca tctggaataa ccagcaccgg ataatggacg ccggcgcgat cgatcgcctc
     2581 tgaatcaatg aaatcgaagt tgtaaccggc atcgagaatt gccggtacga gatccgttcc
     2641 gagcagcttt gccatctggc cgctgaccga cgcctcaccg aggcgaaaat gagcctcggc
     2701 atcttcctcc ggaagcagaa ccgcaacatc attggcgggc tggccctggc gcagtaacca
     2761 gctcatgcgc gtcagatacg cagtgacgtc cggcatcaca atccaccagg gattgtggtc
     2821 gttgaagacc gcggcggcat agaacgaata tcctggctcc cccgccgaag ccggagagta
     2881 gggccaccca tggcccacca gctgattgat tccctgcagg aaaaaagtgt cggcctccgc
     2941 tttcatgtcc aggggtgtcg cgctgaaggc aggcgaatgc agccacgtcc aggtctccga
     3001 cgaagtcaca ggccgtccgt aaacatggct cgccgacgtg gcccagcgcg taaacgagaa
     3061 tggcccgtgc cactgcgatc cctctccctc cggcaaatcg acgcgcgaat tgctcgacaa
     3121 cgtaaccgcg gggctgccgt aggtctgaga tcgaaactca gtgtggtgcg cgtgcgccca
     3181 ggcattaatc gctcccagat aattctggtc gatcaactcc gccagcgtgc gtccccagtc
     3241 atagcgcaga tccgccgcct ccgggcctgt tccagcaaca agctctggca gataaggcgt
     3301 caaatcgtaa ccgcgacgat tgcgaaattc gctcaggaag tccgccgtcc aatccgattc
     3361 atagacctcc agactgtcgc tgaaaacgga atacggcgga tgatcgccaa acgcccccac
     3421 gatcggatcg gccacattcc tcaggtgatc ctgtacggca cccgcgctga agtgatcgat
     3481 gacaaacccc tccgctccgt aagccggccg cttgacctgt tgcccagtgc ggctggagat
     3541 aaaaaacagc gcaacgcgcg gatcgcccaa atctgaggga agctgcaaac gtccgtccct
     3601 gacgtcttcg acccgccgcg cgtgatctgc gtcgtagcgt tccgacgtcc cggcagccac
     3661 gaacgacgcc aggagcgtct ccccattttc gagtgaaggc acagcgactg aatgcagccc
     3721 agccggaact gcaaccgcca ccacccgcaa acgccccgcc gactccgtca ccggtaccca
     3781 cgcaccaccg tagggccacc cactgcccag cgtaatgctg acccgcatcc ccagttcatg
     3841 gccggtgttc gcagcaaagg tgaccatctt caggaattcc ggcgaaagga atttcaggtt
     3901 gcggaatcct ttttgcggat cgtcaagctg gagcggatac accggttgaa tctccacgcc
     3961 gccgatgccg ccggccttca tcgtgcgcag ttcgcgcgca agctcaccgg gctcgacggc
     4021 tggcccgaac caccaccagc gcatcatggg tcgcgcttcc attggaggcg cggcgaaacc
     4081 ccggcgcaaa gaggcgagag aatcgccggc atttgtggga cgccgtgtcg tcgtagaatt
     4141 ctgctgcgca ccaaccagac tggcggccgt gagacaaagg gcggccccgc agggcatcag
     4201 ctttcgaaca gcgatcatga atcgggagca tctccaacgc cgttgatcgt acgccacgta
     4261 cgcgcgtgaa ttcagaacgc accacttcag tgcagattca cgttgccgcc tgccgcctta
     4321 atgattcctg agccatcggc agcctcgacg ctgaccctct ggcctgatac atcggcattg
     4381 ctgatcgcca taccgtgctg cgcctgaatg tgcacatcct tcagaaccac attgcgaatc
     4441 ggagcttccg gcaggcctgc aatcgcgcca gcggaaacaa tatccgtggc cgttacattt
     4501 tccaaagtga tgtcatggaa gtggggggtc agccgtgtaa ctggctgcgc tgggtcggga
     4561 tcggggggca gaattttcgg gtagtactcg ctgatgatga tggcgttctt cacatttttc
     4621 atgtcgatgt cgcggaaaac gaagtttccc acgtcattgc cccggtcccg gttggccttg
     4681 acgcggatgc cgttgtccgt cccgtcgaaa tggatacgtt ctgcacggac attctgcgcc
     4741 ccgcccgcca cctcgctgcc aatcgaaagg ccgtgcccgt gcaaaaagtt gcagtcggta
     4801 atggtgatat cgcggctggg gtcatccggg ccgggcgaat tagcaatacc cgacttgatc
     4861 gcgacatcgt cgtcacccac atccgcgaaa acatgatcga tgcgaacgtg cgaagacgag
     4921 aagggatcga tggcatcggt gttcggcgag tgctgcggcg ccagtactct gatattccga
     4981 atcgtcacat cgtcggagta gtagggcacc agctgccaca tgggtgagtt ctggatcgta
     5041 acgccctcaa ccagcacgtg cctgcagtgg tcaaatacga cgaggcgcgg gcgtgtgtgg
     5101 tcagacccca tcacaccgtg gtccttcacc gcacgagcct catcccacca gctctgtcca
     5161 tttccgtcga tggttccctc gcctgttatc gagacgttgg tcgcattcgt cgcgcttacc
     5221 agcgatcgcc gccccggcgc tttgaactcc gttattgacg gatagtcgtc gtgatccgga
     5281 gaacccagca gcgtcgcccc cttgtcgagt tggagagtaa tcttgctctt cagcatgatc
     5341 ggcgcactga gccaggtacc cgcggagagc ctcaccgtgc cgcctccacg ctgctcgcac
     5401 gcatcgaccg cacgctgaat cgcggtcgtg tccctggcta tgccatctgc tttcgcaccg
     5461 tatgcatgcg gatcgcaaac ggtggacaca gccgcagccc atgaacggct tccggcagcc
     5521 agtcccagca caaccaatcc ggacaagaca ggcaaaaagc caaatcgaat tcgcatcagg
     5581 agaatttcct ctgggggttc agttccaatt gcgaaaccca tgatacgctg gacggcgctc
     5641 agtggtcata ccactatgtg ggaccgtgga ttgcaatcga gacactactt ttgcgaggat
     5701 cgtgcgctat gaactctttc cgctccaatc gtcgagaaat gctccgcatg agtgctctgg
     5761 gactcggtgc cgtggtcggt tcttccctgc cagaggcgta tgcgcttccc cgctctcccg
     5821 ctccgtcgca gggcatgccc ggcatttttg acgtgcgcac ctatggtgcc gtcggcgacg
     5881 gaaagaccgt ggacacacct gccatcaata aggcgattga ggcagctgcc gcagcgggtg
     5941 gaggaacagt cgtatttcct ccaggcacct ggctcagctt ctccatccac ctgaaaagtc
     6001 acgtaggact ttacctcgcg caaagcgcga cgctcctcgc cgccgactct ccgcttccag
     6061 gacaaaccac cggttacaac ggaggaacct atgatgcggc tgagccgaac acaccttggg
     6121 aggactacca ggactatggc cataaccact ggcacaactc gctgatctgg ggtgagaatc
     6181 tctccgacat cagcatcacg ggtccgggaa cgatctacgg caagggcctg agtttcggag
     6241 cccgccgcaa cgcccgcggc aattatccga tctatgtggc cgagcaggcc ggggtcggca
     6301 acaagagcat cgctctgaag aattgccaca acgtgctgct ccgcgacttt tcgattctga
     6361 agggtggaca cttcggcctt ttgctaaccg gcgtcgataa cctcaccatc gacaacctga
     6421 ccatcgatac ggatcgcgac ggcatcgaca tcgattgctg ccgcaacgtt catgtctcca
     6481 actgcaccgt gaactcaccc tgggacgacg gcatttgccc aaaaagcagt tacgccctcg
     6541 gctatgcgcg cgcgaccgaa aacgtgacca tcaccaattg ctatgtgacg ggtgactacg
     6601 aactcggcac cgttctcgac ggcacattta aacggttcgc ggccgatgca catgtgggcc
     6661 gcacaggccg catcaagtgc gggacggagt caaatggcgg atttaaaaat atcaccatct
     6721 ccaactgcgt gttcgatacg tgtaacggcc tggccctcga gagcgaagac ggagcacttt
     6781 gcgaagatat tgccgtcacg aacatcacca tgcgcaatat caccacttcc ccaatcttcg
     6841 ttcgtctcgg agcccggctg cgcggcccga aggagacgac caaagtgggc acgatgcgcc
     6901 gcatcctgat cagtaacctg accagttaca actgtgcttc cagactctgt tcgatcctca
     6961 gcggcatccc ggactacgcc atcgaggacg tgaagctggc caacatttac cagcatcatg
     7021 tgggcggtgc gtcgcaggat acgctcgcaa ttcaaccccc ggaaaacgaa aacaagtatc
     7081 cggacccggg tatgtttggg cccatgccat cccagggatt cttcctccgg catattaaga
     7141 acctcgagat gagtcacgta gaagttgccg cagcaacgtc cgatccgcgc cccagctttt
     7201 atctcgccgg cgtcgaccgg gctgatttca ttgccgtcac tgcgcccaaa ggtgtgcagg
     7261 cctttcatct tggcaaggta actgacctgc gcattctgtt gagccgagcg gcaaaagata
     7321 cgcaattgga taccgccgac aaccagacga tctaagaagc aaacgcttgt ctcacgcagt
     7381 ggtctgacca aaatgggcaa agtcgacgtt tctgcaggca tcgactgccg ctttcggcac
     7441 gggcttccgg acagtgggct tcgggcctgg cgggatcgca cttctcctga tgtcgcgagc
     7501 ccgcccttgc acgaaacatt tgcccaatcg cggataaaaa ctggcgcgtt cagccgtaag
     7561 gcgatgaaac cggtcagaaa aacggtccgg gcgtactctg cgcacgcctc tttcacaagg
     7621 cccccccaaa cctccgttcc cggcaccgag acagcgccaa tcataggtct tgacagtctt
     7681 gtaaagcctc cacgatactg gtgcggtttt taagacaggt cagacctctc ggcgcagcca
     7741 tgacctgcac acccaataaa gtttcggagg atacgtcatg acgcagacga accggcgggc
     7801 tcccgtaggg gggcgatcgc ttctttgggc tctcgcactc tgtctgatga ctgcgcccgc
     7861 gttcgcgcag ttagcaggca ccggcaccat ccagggcacc atcagtgatc cttctggtgc
     7921 cgttgtcccg ggtgccgagg taacggtcac cggtacggcc accagcgcga agcatcagac
     7981 cgtcagcagc ggcggcggag tgttcagctt cccgaatctc cccatcggca cctataacct
     8041 ctccgtcacg gcgtccggat tcaaggccta tacccagtcg ggcatcgtcc ttgaagttgg
     8101 aagcaacatc ggagtcaatg tcaacttgtc ggttggcgcc gcgaaccagg aagttcacgt
     8161 ccaggccaat ggcatcgccc tgcagacgca ggacacctcc ttcaagcaga cgattgacca
     8221 aaaagaggtc aatgagttgc ccctcaatgg ccgccagatg accgacctga tcgtgctttc
     8281 tggcggagct gcgagtgctc ccggtaacga tatggtcggc agtaagaa
//
LOCUS       DASZYN010000811         2924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_752804, whole genome shotgun sequence.
ACCESSION   DASZYN010000811 DASZYN010000000
VERSION     DASZYN010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752804"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..907
                     /gene="nadC"
                     /locus_tag="VHE33_20375"
     CDS             23..907
                     /gene="nadC"
                     /locus_tag="VHE33_20375"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HVT99867.1"
                     /translation="MEWKSRRIEAILEQALAEDKATSDVTTALTIEPSLRASATIIAK
                     EDCVLSGLGCIPRFLDIFARLDAKSSGRHEVISHPEIFDGVRLRNGQAVAVIRHNARV
                     ILACERVILNLMQRMSGIATLTRRYVDEIDGTGVKILDTRKTIPGLRILDKYAVRCGG
                     GENHRLDLSDGILIKNNHITLGGGIVKVLERAKERRKPGQTIDIEVRNPTELELALDH
                     GAESLLLDNMTPAEVKKSIEFVRERGLKIPIEASGGITLENIRKYALAGPDYISVGAL
                     THSAQAVDLSMRITAEIY"
     gene            912..1730
                     /locus_tag="VHE33_20380"
     CDS             912..1730
                     /locus_tag="VHE33_20380"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HVT99868.1"
                     /translation="MADPSFTPFDDPFDLPALNAALAGSRFAGNLHFFPSIHSTNSHA
                     MQQADSGAPDGSIYLADEQTAGRGRGAHAWTSPPGSGLYVSILLRPRIAPADILWLSL
                     AAGLAVHDAIRTVTSLQSDLRWPNDLLFGSRKFCGILTELNAEVTRVRHAVIGVGINV
                     HQPLFPAEISSVATSLFIETGRNWPRQDLLLALLTSLNRELAALADPSIAAESIRTRL
                     EQRSSWIRGKRVLVDEGEPYTGVTDGLDTRGFLRVHTKNGLRTVLSGGVREARA"
     gene            1770..2606
                     /locus_tag="VHE33_20385"
     CDS             1770..2606
                     /locus_tag="VHE33_20385"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HVT99869.1"
                     /translation="MLLVLNVNNTNTVIALYPLTPDGHTGDLRATWRISTRQSQTADE
                     YGILIRSLLQHEGVESGAIRGIVIASVVPPIDWILRQFCERYFTQKPLFIEPGVKTGL
                     PILTDNPGEVGADRVANCVGAFHRYGGPTIVVDLGTATNFDVVSKKGEFLGGAIAPGL
                     AISADALFSRAARLPRVEIRRPAKIIGTNTVDNLQIGLFWGHIGLVDSILERMIGELG
                     SETKCIATGGLAHLIARESKFITEVNDRLTLEGLRLIYDRNRAARSASHSESHSTRPG
                     AD"
     gene            2611..>2924
                     /locus_tag="VHE33_20390"
     CDS             2611..>2924
                     /locus_tag="VHE33_20390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99870.1"
                     /translation="MENYFNYFTEIEEHFQRRRGSILLLSTLDWALIDTWREAGIPLE
                     AVLRGIDTAFDRYEARKHRTVRRVNGLAWCAQEVLTAAEDMREASLGAARQKAEPANA
                     GL"
BASE COUNT          562 a         1071 c          761 g          530 t
ORIGIN      
        1 cccggagggc gctaactaac ccatggaatg gaagagccgc cgcatcgaag ccattcttga
       61 gcaggccctc gctgaggaca aagccaccag cgatgtcacc accgctctga ccatcgagcc
      121 ctcgctgcgc gcctctgcaa ccatcatcgc taaagaagac tgcgttctct ctggcctcgg
      181 ctgcatcccg cgcttcctcg acatcttcgc gcgcctcgat gccaaatcga gcggacgtca
      241 cgaagtcatc agccatccgg agatcttcga cggcgtccgc cttcgcaatg gccaggccgt
      301 cgccgtcatt cgtcacaacg cccgtgtcat tctcgcctgc gagcgtgtca tcctcaacct
      361 catgcagcgc atgagcggca tcgccaccct cactcgccgg tacgtcgatg agatcgacgg
      421 caccggggtc aagatcctgg acacgcgcaa gaccatcccc ggactgcgca tcctcgacaa
      481 gtacgcggtc cgctgcggcg gcggcgaaaa ccaccgtctc gatctctccg acggcattct
      541 tatcaagaac aaccacatca ccctcggcgg cggaatcgtg aaagtcctcg agcgcgcaaa
      601 agagcgccgc aaaccaggcc agacgatcga catcgaagtt cgcaatccga ccgaactcga
      661 gctggccctc gaccacggcg ccgaatcgct gctgctcgac aacatgaccc cggccgaagt
      721 gaagaagtcc atcgagttcg tgcgcgagcg cggcctgaag atccccatcg aagcctccgg
      781 cggcatcacg ctcgaaaaca tccgcaagta cgccctcgct ggcccggact acatctccgt
      841 cggtgctctc acccactccg ctcaggccgt cgacctctcc atgcgcatca cggcggagat
      901 ctattagatc ggtggcagat cccagcttca cccccttcga tgatcccttc gatctgcccg
      961 cgctgaacgc cgcgcttgcc ggatcacgtt ttgccggcaa cctccacttc ttcccgtcga
     1021 tccactcaac gaactcccat gccatgcagc aagcggattc cggtgcgccc gacggcagca
     1081 tctatctggc cgacgagcaa accgcaggac gaggccgagg cgcgcacgcc tggacctcac
     1141 ctcccggctc aggcctctac gtcagcattc tcctgcgccc acgcatcgcg cccgccgaca
     1201 tcctctggct ttcccttgct gccggtctcg ccgtccacga cgcgatccgg accgtcacat
     1261 cgctccagtc cgatctgcgc tggcccaacg acctcctctt cggctcacga aaattctgcg
     1321 gcatcctcac cgaactcaat gccgaggtca cgcgcgtccg ccacgccgtc atcggcgtcg
     1381 gtatcaacgt tcaccaaccg ctgttcccag ccgaaatcag ctcggtcgct acatcgctgt
     1441 ttatcgaaac tggccgtaac tggccgcgcc aggacttgct tctcgcactg ttaacgtctc
     1501 ttaaccgcga actcgccgcc cttgccgacc cgtcaatcgc cgccgaaagt atccgcaccc
     1561 gtctcgagca gcgttcctca tggatccgcg gcaagcgcgt actcgtcgac gagggtgagc
     1621 cgtacaccgg cgtcaccgac ggcctcgata cccgcggctt tctccgggtt catacgaaga
     1681 acggcctgcg caccgtgctt tccggcggcg ttcgcgaggc cagggcatag aatcgagaac
     1741 gcgcaaactg cgcgcataca atcagagcca tgctgcttgt cctcaacgtc aacaacacca
     1801 acaccgtgat tgcgctctac ccgctcacgc ccgacggcca tactggtgat ctgcgcgcga
     1861 cctggcgcat cagcacgcgc cagtcgcaga ccgcggacga atacggcatc ctcatccgca
     1921 gccttctgca gcatgaaggc gtcgaatcag gcgccatccg tggcatcgtg attgcctccg
     1981 tcgttccccc catcgactgg atcctccgcc agttttgcga gcgttacttc acccagaagc
     2041 cgctgttcat tgagcccggc gtcaagactg gtttgcccat tctgacggac aatcccggcg
     2101 aagtgggcgc tgatcgggtc gccaactgtg tcggcgcgtt tcaccgctac ggcggcccca
     2161 ccatcgtggt tgaccttggt accgcaacca acttcgatgt cgtctcaaag aaaggcgaat
     2221 tcctcggcgg cgctattgcg ccaggcctcg ccatctccgc cgatgcgctc ttctcccgtg
     2281 ccgcacgcct gccccgcgtc gagattcgcc ggcctgcgaa aatcatcggc accaacaccg
     2341 tcgataacct ccagatcggc ctcttctggg gtcatatcgg tctggtcgac tccattctcg
     2401 agcgcatgat cggcgaactc gggtcggaga cgaaatgtat cgccacaggc ggcctcgccc
     2461 acctcatcgc gcgcgaatcg aaattcatca ccgaggtcaa cgaccgcctc accctggaag
     2521 gcctccgcct catctacgac cgcaaccgcg ccgcccgcag tgcatcgcac tccgaatcgc
     2581 actcgacccg tcccggcgcc gactgacgcc gtggagaatt acttcaacta cttcaccgag
     2641 attgaggagc acttccagcg ccgccgtggc tccatccttc ttctgtccac gctggactgg
     2701 gctctgatcg acacgtggcg cgaagccgga attccgctcg aagccgtgtt gcgcggcatt
     2761 gataccgctt tcgaccgcta tgaagcccgc aaacatcgca ccgttcgccg cgtcaacggc
     2821 ctggcctggt gcgcccagga agtattaact gccgcggagg acatgcgcga ggcatctctc
     2881 ggggcagcca ggcaaaaagc tgagcctgcc aacgcgggtc tcga
//
LOCUS       DASZYN010000812         1972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_752881, whole genome shotgun sequence.
ACCESSION   DASZYN010000812 DASZYN010000000
VERSION     DASZYN010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752881"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..>1972)
                     /locus_tag="VHE33_20395"
     CDS             complement(214..>1972)
                     /locus_tag="VHE33_20395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99871.1"
                     /translation="RYFTETDDDSVDDLYAIAQREVPLYIEQLRAHGYPYDALIINAG
                     GFFIDNAPPDTRWCQVIERWNREHEKIRLRTATVSEWFAALPEHGNTDYPGYQVAWPD
                     SWAHGLGSCTARVAQIRRSQRRRADVEALVQRAQTPRAAAYFARALEEERMALEHTFG
                     AWSTTRRPTSWLNNFEQNVKELFFHRSELYLNEAAGAALRTGPRLKDRPPTLYAGGSA
                     HEAGAHLLHFTSEELLPDPSTQVLRARDGQQYSFQRENEELAQFVAVVPLPSGELSAF
                     ELVAGQAAEIAAPFQMERQGHEQVIETAAWRARLDLRTGSLRSLVERASGHEWINRTH
                     DYGFGQLVHERVIHPWGWRAVGNEQRFLALGIASEKLKESYPEGPVFARETVEIAEEP
                     ALVRGAIFTEVRLRAQEASFGKVQLGWRFYHHLPLVELVIDWDKVWSEVPEAGYVAFP
                     FAGEQASLDLETGGGFFRPGSHETGGQLPGTCASYYTMQRAARIGGAQGAQALWLPLD
                     APLVMTNELNYNRWETEPWTWNGFLASMPVNHYWHTNFPVSQQGPFRLRYRLLSTAGM
                     SSEEQAIQAAQPVEALGWH"
BASE COUNT          324 a          640 c          650 g          358 t
ORIGIN      
        1 tactaacatg cgcacgagat cgggcgccgg atcgtgcggg tccagcgtct cgacatgttg
       61 agccagcatc tccgtgccgc gtaccaggcc ggctttgatc cgcgtgccgc ccacatcgaa
      121 ggcaagaacc acctcctgct cgtcaggctc gcgcgcctcg ctcattccat gcatctctcg
      181 taacggactc accgctcttt tccccctctc gtcctagtgc cagccgaggg cttcgacggg
      241 ctgggcggcc tggatggcct gttcctcgct gctcatgccc gccgtactca gcagacgata
      301 gcgcaggcgg aacggtccct gctggctgac cgggaaattg gtgtgccagt aatgattgac
      361 cggcatcgag gccaggaagc cattccaggt ccacggctct gtctcccagc ggttatagtt
      421 caactcgttg gtcatgacca gcggggcatc caggggcagc cagagcgcct gcgcaccctg
      481 cgcgccgcca atgcgtgccg cccgctgcat ggtgtagtag ctcgcacagg tacctggcaa
      541 ctggccgccc gtctcatgac tgcctggccg gaagaagccg ccgcccgtct ccaggtccag
      601 gctggcctgc tccccggcga aggggaaggc cacatagccg gcttcgggca cctcgctcca
      661 caccttatcc caatcgatga ccagttccac cagaggtaga tgatgataga agcgccagcc
      721 cagctgaacc ttaccgaacg aggcttcctg ggcgcgcagg cgcacctcgg taaagatcgc
      781 cccacgcacc agggccggtt cctctgcgat ttccaccgtc tcgcgggcga agaccggtcc
      841 ctcgggatag ctctctttga gcttctcgct ggcgatcccc agagcgagga agcgctgttc
      901 attgcccacg gcccgccagc cccaggggtg gatgacgcgt tcatgcacca gttgtccaaa
      961 gccgtagtcg tgcgtgcggt tgatccattc gtggccgctg gcacgctcga ccaggctgcg
     1021 cagactgccg gtgcgcagat ccagtcgtgc ccgccacgcc gctgtttcaa tcacctgctc
     1081 gtggccctgc cgctccatct ggaacggcgc cgcgatctcc gctgcctgtc cggccaccag
     1141 ctcgaacgca ctcagttccc cggagggcag cggcaccacg gccacaaact gggctaactc
     1201 ctcgttctcg cgctggaagc tgtactgctg accgtcgcgg gcacgcaaga cctgggtgga
     1261 aggatcgggc agcagttcct cggaggtgaa gtgcagcagg tgcgcgccag cttcgtgggc
     1321 gctgccgccg gcgtagaggg tgggtggccg atccttgagg cgaggaccgg tgcgcagggc
     1381 ggcccccgcc gcttcgttga ggtagagttc cgagcggtgg aagaagagtt ctttgacgtt
     1441 ctgctcgaag ttgttgagcc aggaggtggg acggcgggtg gtggaccagg cgccgaaggt
     1501 gtgttccagg gccatgcgtt cttcttccag ggcgcgggcg aagtaggcgg cggcgcgcgg
     1561 cgtctgagcg cgctggacca gggcttcgac atcggcgcga cggcgctggg agcggcggat
     1621 ctgcgcgaca cgggcggtgc aggaacccag gccgtgagcc caggaatcgg gccaggccac
     1681 ctggtagccg gggtagtccg tgttgccgtg ttcagggagc gcggcgaacc actcgctgac
     1741 cgtggcggtg cgcaggcgga tcttctcgtg ctcgcggttc cagcgttcga tgacctggca
     1801 ccagcgcgta tccggtggcg cattgtcgat gaaaaagcca ccggcgttga taatcagcgc
     1861 gtcgtaggga tagccgtgag cgcgcaactg ctcaatgtag agcggcactt cgcgctgcgc
     1921 gatagcgtac aggtcgtcca cgctatcatc gtcggtttcg gtgaaatagc gc
//
LOCUS       DASZYN010000813         5321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_115647, whole genome shotgun sequence.
ACCESSION   DASZYN010000813 DASZYN010000000
VERSION     DASZYN010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115647"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..2664)
                     /locus_tag="VHE33_20400"
     CDS             complement(7..2664)
                     /locus_tag="VHE33_20400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT99872.1"
                     /translation="MSLRSRFTTWWAAVFHNDVLNNQLSDELAFHIESRAEELMRSGL
                     SREQAHRRARAELGSLAAARENLRQAWGTRFFDELRGDLRYAVRILTRSPGFSAVAVG
                     SLALGIGANTVIFSMVNAVLLRPLPFPHGEQLTAVMEANEHEGGGDTGSSFPDYSAWR
                     AQTRTFSAMAGYQFHDLTLTGFGEPSVLHTVVTTSSLFPLLGINPVVGRTFSEKDGER
                     GAAAVVVLSEQLWRSQLAANPSILGKTITLDQRPFTVIGIMPASFHYPLSQSKQDLWI
                     PLAQDPLFGPWMTRPGGHWLRVVGRLRDGVTLKQARAEMVALEAGRALADPADDTGWT
                     VHLKPLHEAVTSNVRLALWVVLGAVSLVLLIACANIANLLLARATARSKEMAMRVALG
                     ADRRRIARQLLTESAVLGLAGAALGVALAWMAIETLRAALAERLPQIHTIAVDRRVLF
                     FALVLSVATSLLFGLAPVLFAGRSDPQMDLAAGARSGVAQGPQRARKALAVVQIALAM
                     MLLAGTGLLMRSFLRLTSVAPGFIANGLVKASISLPRFQYSTPQQWSTFSVDLLRRLH
                     EQPGLRQSAMVAPLPILDGFVNLGFLVEGAPPPPPGVSQTADYVAASPEYFSVMGIPL
                     QRGRRFDVDDGPSRPPVALISAAFAQRYFPHENPLGRRVMIGFPPDGNVAREVVGVVG
                     DVRDATLSQNPGPMVYVPFAQAPFWGGELVVKSSMDVAAVAAALREQTKAIDRGLPVT
                     DVESLAAAMNDSVTEERFRTRLLAGFSALALLLAAGGIFGVMSYSVSRRTNEIGIRMA
                     LGAQPRRVLRMVLSEASWMVAFGVVAGLAGALALGRLISTMLYGLAPWDPATFALAGG
                     VLIAVALAASWIPARRAAGVDPMKALRHE"
     gene            complement(2695..>5321)
                     /locus_tag="VHE33_20405"
     CDS             complement(2695..>5321)
                     /locus_tag="VHE33_20405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HVT99873.1"
                     /translation="VPREEALRRARAELGSLAAVRENSRQAWGTRFLDELRGDLRLAI
                     RVLAKSPGFTAIAIGSLALGIGANTAIFTAAQHMLLDRLDVPHPEQLRLFGWTAPRNG
                     VVESLWGQWDDLPGGGMVSTSFSFPVYRQLRRENRSLADVFAFKGFGRMVATVDGHAQ
                     PVGVEMVSGNYYAGLEVKPQLGRAIEESDDGAVGSGPVVTISDGFWARQFGRSPDVIG
                     KTILVDAVPMTIVGVNPRGFTGAYSAQESPDIFLPFSMEPIVAPEELSDGKESLLQNR
                     SIWWVLVMGRLKPGVPPAAANAALNVALQAAVRTTMTVPAGKAIPRLLLRDGSRGQNP
                     YADSMVKPVAVLMGLAGFVLLLACANLANLLLARTGARQREMSVRLALGAGRARILRQ
                     MLTESLLVSLLGGAAGILIAWAGHNAIPRLFAHAWEQPPFAAHFNWPIFLFAAAISVL
                     TGLMFGMAPAWQAARVQVSSGLKDSGQTVTHRRRGLGGRAIVVVQIALSLLLVVIAGL
                     FVQTLMKLGRAPLGFEPHQRLLFSLQIPQSSDAPASLRLIQQIENRLSALPGVQSVTA
                     MTVPLISGNVSTLTFVPEGEHYARDSRPRVEENTVGNGFFTTFGIPLIAGRSFNPEDT
                     PASRKVAIINQSLARKFFSDRNPVGTTFTAGWNHPYLVQIVGVCADAKYNSLRDKPEP
                     TVYLPLAQRAGGLTEPTFAIATSLAGESILPSVRDAVASVDRNVPVLDVRTQDEQIAA
                     SLQQPKIFAGMTTGFGVLALLLASIGIYGIMAYSVSRRTNEIGIRMALGAERGRVLRM
                     VVGEAFWMVAIGIAAGLAAALTLGRLIGSMLYGLKPWDPATYALSGALLILVALAATW
                     IPAHRAAGVDPMKALRHE"
BASE COUNT         1029 a         1643 c         1784 g          865 t
ORIGIN      
        1 cttttctcac tcgtgacgca gggctttcat gggatcgaca cctgccgcgc ggcgagcggg
       61 aatccaactg gcggcgagag cgacggcgat cagcactcca ccggcgaggg cgaaggttgc
      121 cggatcccag ggtgcgaggc cgtagagcat cgtggagatg aggcgcccga gagcgagagc
      181 ccctgcgagg ccagctacga cgccgaacgc caccatccag gacgcttcgc tgagcaccat
      241 tcgcagcacg cggcgaggct gcgcgccaag ggccatgcgg atgccgattt cgtttgtgcg
      301 gcgggatacg gagtacgaca tcacgccgaa gattccgccc gcggcgagca gcagcgccaa
      361 cgcgctgaat ccggccagca aacgtgtgcg gaagcgctcc tcggtcacgg agtcgttcat
      421 ggcggcggcc aacgactcca cgtccgtcac cgggagcccg cggtcgatcg ctttggtttg
      481 ctcgcgcagg gctgcggcga cggccgcgac atccatgctg cttttaacga cgagttcacc
      541 accccagaac ggcgcctgcg caaacgggac gtacaccatc ggcccggggt tttggctgag
      601 ggtcgcatcg cgcacatcgc cgaccacgcc cactacttcg cgcgcgacgt ttccatcggg
      661 agggaatccg atcatcacgc ggcgtccaag gggattctcg tgagggaaat atctttgtgc
      721 gaaggcggcg ctgatcaggg ctacgggagg ccggctgggt ccgtcgtcga catcgaaccg
      781 gcggccacgc tgcaaaggaa tccccatgac cgagaaatat tcggggctgg cggcaacgta
      841 gtcggctgtc tgtgacactc cgggcggcgg cggcggagcg ccctcgacga gaaaaccgag
      901 attgacaaag ccgtcgagga tcggcagagg cgcaaccatg gccgactgcc ggagaccggg
      961 ctgctcgtgc aggcggcgca gcagatcgac agagaacgta ctccactgct gcggagtgga
     1021 gtactggaac cgtggcagag agatggaagc tttcaccagt ccgttcgcaa tgaatcccgg
     1081 cgcaacggat gtgaggcgaa gaaagctgcg catcagcagg ccggtgccgg cgagcagcat
     1141 catggcgagc gcgatttgca cgacagcaag agctttgcga gcgcgttgcg gtccctgcgc
     1201 tacccccgac cgcgctcccg cagccagatc catttgggga tcggagcggc cggcaaacaa
     1261 gaccggagcc aaaccgaaga gaaggctggt agcgacggat aaaacaagcg caaagaacag
     1321 tacccggcgg tccacagcga tggtgtggat ttgcggcagg cgctccgcga gtgcagctcg
     1381 cagcgtttcg atcgccatcc acgccagcgc cacgccgagg gcggcaccgg ccaggcccag
     1441 gacggctgat tcagtaagca gttggcgtgc aatgcgacga cgatcggcgc cgagagcgac
     1501 gcgcatggcc atctctttgc ttcgcgctgt tgcccgggcc agaagaagat tcgcgatatt
     1561 cgcgcatgcg atgagaagca ccaggctgac tgcgccgagg acgacccaca gggcgaggcg
     1621 gacattgctg gtgaccgcct cgtgcagcgg cttgagatgc acggtccatc cggtgtcgtc
     1681 ggcaggatcg gccagagcgc gaccagcctc aagagcgacc atttccgcgc gcgcctgttt
     1741 gagcgtgacg ccgtcccgca gacgcccgac cacccgcagc cagtgtcctc cggggcgggt
     1801 catccaggga ccaaagagag gatcctgggc gaggggaatc cagaggtcct gcttcgactg
     1861 gctcagcggg tagtggaaac ttgcgggcat gatgccgatc acagtgaacg ggcgctggtc
     1921 gagggtgatg gttttgccca gaatcgatgg attggcggca agctggcttc gccatagctg
     1981 ctcgctcagg acaacgaccg cagctgctcc acgctctcca tctttttcag agaacgttct
     2041 gccaacaacg ggattgatgc caagcagggg aaacaggctc gaggtggtga cgacggtatg
     2101 cagaacgctt ggctcaccga atcctgtgag cgtgaggtcg tggaactggt aacccgccat
     2161 cgcactgaag gtgcgggttt gcgcgcgcca tgctgaatag tcgggaaaag aagagccggt
     2221 gtcgcctccg ccctcatgct cgttcgcctc catgacggcg gtgagttgtt cgccatgagg
     2281 aaagggaagc ggtcgcagga ggacagcgtt gaccatggaa aagatgacgg tgttggctcc
     2341 gataccgagc gcgagagagc cgacggcaac ggcggagaag cccggactcc tggtcaaaat
     2401 gcgaacggca tagcgcagat cgccgcgtag ctcgtcgaag aagcgcgtac cccaggcctg
     2461 gcgcaaattt tctcgcgccg cggcgagact gccgagttcg gcgcgagcgc ggcgatgagc
     2521 ttgttcgcgg gacagcccgc tgcgcatgag ctcttcagcg cggctctcga tgtggaaggc
     2581 cagttcgtcg ctcagttggt tgttcagcac gtcgttgtgg aagacggctg cccaccaggt
     2641 cgtgaatcgg ctgcgcaggc tcatggctcc tccctcatgg tgttttgcgg gcgttcattc
     2701 gtgacgcagg gctttcatgg gatcgacacc tgcggcccgg tgggccggga tccacgtagc
     2761 cgcgagcgcc acgaggatca gcagtgcgcc cgacagcgcg taggtcgccg gatcccacgg
     2821 tttcaaaccg tagagcatcg agccaatgag acggcccagc gtcagggcag cggccagacc
     2881 ggcagcgatc ccgatggcga ccatccagaa ggcttcgccg accaccatcc ggagcacacg
     2941 cccgcgctcg gcgccgagtg ccatgcggat gccgatctcg ttggtgcggc gggaaaccga
     3001 atacgccatg atgccgtaga ttcctatgga agccagcagc agcgccagca ctccaaatcc
     3061 cgtggtcatg cccgcgaaga tcttcggttg ctgaagactt gccgcaatct gctcatcctg
     3121 ggtgcgcacg tcgagaaccg ggacgttgcg gtcgactgat gcgaccgcgt cccgcacgga
     3181 gggcagaatg gattcgccgg cgagacttgt ggcaatggca aaggtgggct cggtcagtcc
     3241 tccggctctt tgcgcgaggg gcagatagac ggtcggctcc ggcttgtccc gcagcgagtt
     3301 gtactttgca tccgcgcaca cgccgacgat ctgaaccagg tagggatgat tccagccagc
     3361 cgtgaatgtg gtcccgacgg ggttgcgatc ggaaaagaat ttccttgcca ggctttgatt
     3421 gatgatggct actttgcgcg aggctggggt atcttccggg ttgaaacttc ggcccgcgat
     3481 gagtggaatg ccgaaggtag tgaagaagcc gtttcctacg gtgttctctt ccactctcgg
     3541 ccggctgtcg cgcgcgtaat gctcgccttc cggcacaaaa gtgagggtgg agacgttgcc
     3601 cgagatgagc ggcacggtca tggccgtgac ggattgaacg cctggcagcg cactgagccg
     3661 attctcgatc tgctggatca gtcgaaggct ggccggcgca tcgctggatt gcggaatttg
     3721 caggctgaac agcagccgct gatgaggctc gaatccgagc ggggctctgc cgagtttcat
     3781 cagggtctgc acaaacaggc ccgcgatgac caccagcagc agggacaggg cgatctgcac
     3841 gacaacgatg gccctgccgc ccaggccgcg gcgcctgtgc gtcaccgtct gcccgctgtc
     3901 cttcaggccg ctgctcacct gcacgcgcgc cgcctgccat gcgggcgcca taccgaacat
     3961 cagcccggtg agcacggaga tcgcggcggc aaacaggaag atgggccagt tgaaatgcgc
     4021 agcgaacggc ggctgctccc acgcatgcgc gaacaggcgg ggaatcgcgt tgtggccagc
     4081 ccaggcgatc aggatgcccg cggcgccgcc taacagcgaa accagcaggc tctcggtgag
     4141 catctgccgc aggatgcgcg ctcgccctgc gcccagcgcg aggcgtacgc tcatttcgcg
     4201 ctgccgcgcc ccggtgcgag ccagcagcag attcgccagg ttggcgcagg ccagcagcag
     4261 cacaaatcca gccaggccca tcagaaccgc taccggcttc accatgctgt cggcatacgg
     4321 attctggccg cggctcccgt ctctcaggag gaggcgcgga attgccttgc ccgcggggac
     4381 ggtcattgtc gtgcgcaccg cggcctgcaa cgcaacgttc agggccgcat tggcggccgc
     4441 tggcggaacg cccggcttca gacgccccat gaccagcacc caccagatcg agcggttctg
     4501 caggagagat tctttaccgt cgctcaactc ctccggtgcg acgatgggct ccatgctgaa
     4561 gggcaggaag atgtctgggc tctcctgggc tgaataggcg ccggtgaagc cgcgcgggtt
     4621 gacgccgaca atggtcatcg gcaccgcgtc taccagaatc gtctttccga tgacatcggg
     4681 cgaacgtccg aactgcctgg cccagaatcc gtcgctgatc gttaccacgg ggccgctgcc
     4741 gacagcgccg tcgtccgatt cctcaatcgc gcggccgagt tgcggcttca cttcaagacc
     4801 cgcgtagtaa ttgcccgaga ccatctcgac gcccaccggt tgcgcatgcc catcgacggt
     4861 ggcgaccatg cgtccgaaac ctttgaaggc aaagacgtcg gccagcgagc gattctcgcg
     4921 gcgcagctgc cgatacaccg ggaaggagaa cgaggttgaa accataccgc cccccggcag
     4981 atcgtcccat tggccccaca gggattcgac aacaccattg cgcggtgcgg tccagccgaa
     5041 gaggcgcagc tgttccggat gcgggacgtc gagccggtcg aggagcatgt gctgcgcggc
     5101 ggtgaagatc gcggtgttgg cgccgatgcc gagggcgagc gagccgatgg cgatagcggt
     5161 gaagccggga ctctttgcca gaacacggat cgcgaggcgc agatcgccgc gaagctcgtc
     5221 gaggaaacgc gtgccccagg cctggcggga attctcgcgc acggcggcga gactgccgag
     5281 ttcggcgcgg gcacggcgca gagcttcttc gcgcggaaca c
//
LOCUS       DASZYN010000814         7904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1214467, whole genome shotgun sequence.
ACCESSION   DASZYN010000814 DASZYN010000000
VERSION     DASZYN010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7904
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214467"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..744)
                     /locus_tag="VHE33_20410"
     CDS             complement(91..744)
                     /locus_tag="VHE33_20410"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HVT99874.1"
                     /translation="MAHSQQRRGSAGEGKPRHFPGPIWFLALLICISAAALAFVYLDV
                     PVARRVYGVLGSTESLGRGFASAVLLTGEAVVALTLIVIRITRGRLSPLGEATGLACL
                     TSICAYAIDDNALKVFFGVPNPPAVLHGARHTFHLMTGSPGSSFPSGHMVLSGGFAGV
                     FMRLYRRTTLPLAVLQLIGAVLLVVGDWHFVSDVIAGTFVGISAGVLAGEVWAAHSR"
     gene            874..2274
                     /locus_tag="VHE33_20415"
     CDS             874..2274
                     /locus_tag="VHE33_20415"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HVT99875.1"
                     /translation="MSNQEKVLIVEDEPHALSGLAELVSGWGYRTETARDGLEALEKI
                     AAWQPGIIVSDFKMPRMDGLQLLSRLGEATEKVAFVMLTAQGSIEAAVEAMKNGAYDF
                     LQKPVDPTRLRAILTNASRQRDTERELEVTRRKLRDTGVLGSLVGASKKMQEVFALIE
                     RVAPSNVSVLITGESGTGKELVARTLHDLSPRRPKPFVAVNCAAIPETLIESEIFGHE
                     KGAFTGAVERRAGCFELAEDGTLLLDEIGEMPVATQAKLLRVLEDRKLRRLGSKIESP
                     IDVRVLAATNKNPEDAVADGHLRGDLFYRLNVFNIHMPPLREHKEDIPAIVDSMIDDM
                     NQKHQRKVTGIDSEMLARLIAHDWPGNVRELRNTIERAVVLCGDGTLQSRHLPPGFGT
                     KSVRPVAELPPNAIQLEVGATVDDAERMLIFKTLESTHNNKTRAAEILGISLKTLHNK
                     LREYGSTATTAGALEE"
     gene            2347..4368
                     /locus_tag="VHE33_20420"
     CDS             2347..4368
                     /locus_tag="VHE33_20420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVT99876.1"
                     /translation="MRLKTKLVLAITGLVFCVVVVFSWIWLSQLLQQHIEQSWSTTDY
                     LAHNVLYQMRDALDNGLRDRQFDPNDPVAVRAAVADTLRRNRGLNSLLTSIVNYSPTV
                     LDVSIVDRQNRAIIAVPDSTLDDQILPTRPDYAVLRRQSPVRTLKIVFGKPQVYNVSL
                     GLDRNNQPFLTIRIGIRTTFLGSAFKPWLIEAFSFMGLAMIISMIVAAFVANLALQPI
                     EDISQRLDILASQEALTESAASRQVRRGSESELPAGTAPLEGVDRESRRSGDAVAQVS
                     GKIEKLGQRIRNVEEVFSALRENLDQILSNLQDGVMLFTRDARAVLVSSACERFLNIG
                     RERIFGAEVREIFDRSTRLGRLVRDAFEGGMSIVQEEVTTETGRRVEVSLDFIHDTQS
                     RDRHSLGALLTIHDLESVREIETELEVSRRMASIGRLTAGVGHEVKNPINAIVVHLEL
                     LRNKIQVGDDALRHLDIIQSEIRRLDRVVQTLVDFSRPVELQLKDQDLRGVVSTVLML
                     ASADLQTRGITVESQVPSRPVFCRVDADLMEQAVLNVVLNGAQAMASGGALEVRLTED
                     SRWAYVRVRDHGEGIPDDIRNRIFDLYFTTKKEGSGIGLAMTYRILQMHHGQIDVESK
                     AGSGTTFVLRLPLISAGSGQRVVPELTGHAIPSELARQGGETQPERSGP"
     gene            4365..4979
                     /locus_tag="VHE33_20425"
     CDS             4365..4979
                     /locus_tag="VHE33_20425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99877.1"
                     /translation="MSSGKGCAAFAVLLALLTGCGPKKPKAVVPPPTAEVPQLPPSQM
                     AASLPEVPPAIPTTTTHPIKLDTNAPPAVKPEVANTQPHRAPKHHTHPAPDEVKTASS
                     PAQTPAPANQIASTEPSDKTPLGQLSAPGDPVGRQAISEQIDSTENGLNAIKRSLSSD
                     EQKTVTLIRQYITRARDALNANDLDGANTLSSKAKQLLQELTKP"
     gene            complement(5010..5867)
                     /locus_tag="VHE33_20430"
     CDS             complement(5010..5867)
                     /locus_tag="VHE33_20430"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HVT99878.1"
                     /translation="MSRIAIISKPQKEELRQLLPDLVLWLKAHGHEPLLDPVSSTYVL
                     DEPVHPRPELASLNPDLVIVLGGDGTLLAAARVFARSGMPILSVNLGSLGFLTEVRLT
                     DLYAHLEAWGKGVCCVESRAMLHSELWRDGKVYSEHEALNDVVVAKGAIARMGHFAVE
                     VDEQLAASFRADGVIVATPTGSTAYSLAAGGPVMVPRVDVLVVTPICPHQLTLRPMVL
                     QGSSRVLVRIEGIPDQTFLTVDGQEAVELRVGDELQCRKSDYCVKLIRLGENGFFDVL
                     RSKLKWGEK"
     gene            complement(6021..>7904)
                     /locus_tag="VHE33_20435"
     CDS             complement(6021..>7904)
                     /locus_tag="VHE33_20435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99879.1"
                     /translation="IPAQTACLSQGQTLQTSLLSETTITGPGGTTLCSPTTLSCTDPK
                     ANVGTITYNAVTKDVVNINNTTDPTSSTPGSASGQPNPNGAATANLPGSTVINAATSE
                     VTSAAGFFSTCPPKTIALSINGSTSATVNPSSPQTVVAAVTDSNNNPINGLALTYSST
                     EPQNLSVSSTGLVSASFPSHATVTATCQPPTCNPAPVNLIGNLGNGMPVTGNRVNVNS
                     PGRSSNIIWFASSQSPYFSEIDLTTGGAASPIRLPYTPNSMVMDQQGDTLYFGSYHEL
                     MTYSTLTNTLNKEVAGVPGVVLAVSPTGSTVVINDQLRQVIYLYGAQTGTVTSIAGIA
                     SRAQYSPDGTTVYIVGQNPATGQNTMFVNNSSTGWSSYPLTNQPTYSCALEAGGTTAA
                     PAYNPNWDPFCGPALTITVPSVATFLSGTSTAARSYCPNSSSTPPYFPPAGDAGVTTT
                     QLTATADGNHVLGADNTTFSDILLGSSSGAPTGVPVNACPAYNGPALTVTTNPITATL
                     PVTATEIDQVVASPDSSVAFVTYNASGATGALPYYQPLNTGGQGTLSTVQLSSGAQAP
                     VAGVFSPDGAVFFVSTSGDNQVHMVNTGTLTDTQTLDPKLTNAGGTGVPAQFMAVKSR
                     PTT"
BASE COUNT         1536 a         2700 c         2258 g         1410 t
ORIGIN      
        1 cagcgtgtca ccaatgagta gtcagctaca ccgacgcttg gccctccgtc tcaggccccg
       61 aaactgtatc cgcaccgcca cctccggcca ctaccgcgaa tgcgccgccc aaacctcccc
      121 cgccagcact cccgctgaga ttcccacaaa cgtccccgca atcacatcgc tgacaaaatg
      181 ccagtccccc accaccagca gcaccgcccc aatcaactgc agcacagcga gtggcagcgt
      241 ggtccgtcga tagagccgca tgaaaacacc ggcgaagccg ccggagagaa ccatatggcc
      301 cgaggggaag ctgctgcccg gagaaccggt catgagatga aaggtatgcc gcgcgccgtg
      361 caggacagcc gggggattcg gcacgccgaa aaaaaccttc agagcgttgt cgtcgatggc
      421 atacgcgcag atcgaggtca gacatgcgag cccggtcgcc tcacccaggg gcgacaaacg
      481 tccgcgcgtg atccggatga caatcagagt cagggccacc accgcttccc cggtgagcag
      541 aacggcgctg gcaaagcccc gtcccaggct ctcagttgag cccagcactc catagacccg
      601 ccgggctacg ggcacgtcga gatagacgaa cgccaacgcg gctgcggaga tgcaaatcaa
      661 cagtgccagg aaccagatcg gtccaggaaa atgtcgaggt tttccttcgc ccgcgctgcc
      721 gcggcgctgt tgagaatggg ccacattctt ctcccgcaag gacttctccc agaataccgg
      781 caaatttcgt ttccgcagcc gtaagcgttt ctcgccggtt atgcgtcaaa agtggtacaa
      841 ccgaaccgtg acttccgtag tgaaccccca tacatgagta accaggaaaa agttctgatc
      901 gtcgaagacg agccccacgc gctgtcgggc ctggctgagc ttgtctccgg atggggctac
      961 cggacggaaa cggcccgcga cgggctcgaa gccctcgaaa aaatcgccgc ctggcagccg
     1021 ggcatcatcg tctccgattt caaaatgccc cgcatggatg ggctgcaact gctttctcgc
     1081 ctcggcgagg ccaccgagaa ggtcgccttt gtcatgctga ctgctcaggg ctccattgaa
     1141 gccgccgtcg aggccatgaa gaacggagcc tacgacttcc tgcagaagcc ggtcgatccc
     1201 acgcgtctgc gcgccattct gaccaacgcg tcccgccagc gcgacaccga gcgcgaactc
     1261 gaagtcactc gccgcaaact gcgcgacacc ggcgttctcg gctctctggt cggcgcttcc
     1321 aaaaagatgc aggaggtctt cgcgctcatc gaacgcgttg cccccagcaa cgtctccgtg
     1381 ctgatcaccg gcgagagcgg caccggtaag gaactcgtcg cccgcacgct gcatgatctg
     1441 agcccgcgcc gtcccaagcc ctttgtcgcc gtcaactgtg cggccatccc ggagacgctg
     1501 atcgaaagcg agatcttcgg ccatgagaaa ggtgcgttta ccggcgccgt cgaacgccgc
     1561 gccggctgct tcgaactggc tgaagacggc actctgctgc tcgacgaaat cggcgaaatg
     1621 cccgtggcca cccaggccaa gctcctccgc gtgctggaag atcgcaagct gcgccgtctg
     1681 ggcagcaaga tcgagtcgcc catcgatgtc cgcgtcctgg cggctaccaa caagaacccc
     1741 gaagacgctg tcgccgacgg ccatctgcgc ggcgatctct tttaccgcct caatgtcttc
     1801 aacattcaca tgccgccgct gcgcgagcac aaggaagaca tcccagccat cgtcgattcg
     1861 atgatcgacg acatgaacca gaagcaccag cgcaaggtca ccggcatcga cagcgaaatg
     1921 ctcgcccggc tgatcgccca cgactggccc gggaacgtcc gcgagctgcg caataccatc
     1981 gaacgcgccg tcgtcctgtg cggcgacggc acactgcagt cacggcacct gcccccgggt
     2041 ttcggaacca aatctgtccg tcccgtggcc gaactgccgc ccaatgctat ccagctggaa
     2101 gtgggcgcca ccgtcgacga tgccgagcgc atgctgatct tcaagactct cgaatcgacc
     2161 cacaacaaca agacccgggc cgccgagatc ctcggcatca gcctcaagac gttgcacaac
     2221 aagcttcgcg aatacggaag taccgccaca acggctggcg ctctggaaga ataaggtggt
     2281 aagcggggag caatctttgt attctccctt taccgatcct tcactgctga gcggtccccc
     2341 ccgaccatgc ggcttaagac caaactcgtc ctcgccatca ccggactcgt gttttgcgtg
     2401 gtggtggttt tctcctggat ctggctgagc cagctgctcc agcagcacat cgaacaatcc
     2461 tggtcgacga ccgactacct cgcgcacaac gtgctgtacc agatgcgcga tgccctggat
     2521 aacggcctcc gcgaccgcca gttcgatccc aacgatccgg ttgccgtgcg cgctgccgtc
     2581 gccgacacgc ttcgccgcaa ccgcggtctg aattcgctgc tcacctccat cgtcaactat
     2641 tcccccaccg tcctcgacgt ctccattgtg gaccggcaaa accgcgccat catcgccgtt
     2701 ccggactcga ccctcgacga tcagatcctg cccactcgcc ccgactatgc cgtcctgcgc
     2761 cgccagtccc ccgtccgcac gctgaaaatc gtcttcggca aacctcaggt gtacaacgtc
     2821 tctctgggcc tcgatcgcaa caaccagccg ttcctcacca tccgcatcgg tatccgcacc
     2881 acttttctcg gatctgcctt caagccctgg ctgattgagg ccttcagctt catggggctg
     2941 gccatgatca tctccatgat cgtcgccgcc ttcgtggcca acctcgccct ccagccgatc
     3001 gaggacatca gtcagcgcct ggatatcctg gccagccagg aagcccttac cgaatcggcc
     3061 gcctctcgac aggtccggcg cggaagcgag tccgagctcc cggcgggaac tgcccccctc
     3121 gaaggggtgg atcgggaatc tcgccgcagt ggagacgccg tcgcccaggt ttccggcaag
     3181 atcgaaaagc tcggccagcg catccgcaac gtcgaggagg tcttctccgc cctgcgggag
     3241 aacctcgatc agatcctttc caatctccag gacggcgtca tgctgttcac tcgcgacgcc
     3301 cgcgccgtgc ttgtttcgag cgcctgcgaa cgcttcctca acatcggccg cgagcgcatc
     3361 ttcggcgccg aagttcgcga gatcttcgac cgctccaccc gcctcggacg cctcgtccgc
     3421 gatgcctttg aaggcggcat gtccatcgtt caggaagagg tgaccaccga aaccggacgc
     3481 cgcgtcgaag tctccctcga cttcattcat gacacccagt cgcgcgaccg ccattcgctc
     3541 ggggctctgc tgaccatcca tgacctcgaa tccgttcgcg agatcgaaac cgagctggaa
     3601 gtctcgcggc gcatggcatc gattggacgt ctgactgccg gcgtgggtca cgaagtgaag
     3661 aaccccatca acgcgatcgt cgtccacctc gaactgctgc gcaacaagat ccaggttggc
     3721 gacgatgcgc tgcgccacct cgacatcatt cagagcgaga ttcgccgtct ggatcgcgtc
     3781 gtccagactc tggtcgactt ctcccgcccc gtcgagctgc agctcaagga ccaggacctg
     3841 cgcggtgtgg tctcgaccgt gctgatgctg gcctccgccg atctgcagac gcgcggcatc
     3901 acggtcgaga gtcaggtccc atcccgtccc gtattttgcc gcgtcgacgc cgatttgatg
     3961 gaacaggcgg tcctcaacgt ggtcctcaat ggcgcccagg ccatggcttc aggcggagcg
     4021 ctcgaggtcc gcctcaccga agacagccgc tgggcctacg tccgcgtacg cgaccacggc
     4081 gagggcatcc ccgacgacat ccgcaaccgt atcttcgacc tctacttcac taccaaaaag
     4141 gagggcagcg gcatcggtct ggccatgacc tatcgcattc tccagatgca tcatggccaa
     4201 attgacgtag aatctaaagc tgggagcgga acaacatttg tactgcggct ccctttgatt
     4261 tctgccggtt cgggacagcg cgtagtaccg gaactcacag gacacgcgat tcccagcgag
     4321 cttgctcgcc agggtggtga gacccagcca gaaaggtccg gaccatgagt tccggaaaag
     4381 gatgcgcagc gtttgcagtg ctgctggcgc tgctgaccgg ttgcggccct aaaaagccga
     4441 aggccgtcgt gcctccgccc acagcggagg tgccccagct gccgccttcg cagatggccg
     4501 cctcgctgcc ggaagtgcca cccgcaattc ccaccacgac aacgcatccc atcaagctgg
     4561 atacgaacgc gccccccgca gtgaagcctg aggtcgcgaa tacccagccg caccgggccc
     4621 cgaagcacca tacccacccc gctccggatg aggtaaagac cgcctccagt cccgctcaaa
     4681 caccggctcc tgcgaaccaa atcgcatcta ccgaaccctc ggataagact ccactcggac
     4741 aactttccgc ccccggcgac cccgtcggcc gccaggcgat ctccgagcag atcgactcca
     4801 ccgaaaacgg cctgaacgcg atcaagcggt ccctcagctc cgacgaacag aaaaccgtca
     4861 ccctgattcg tcagtacatc acccgcgccc gcgacgccct gaatgcgaac gatctggacg
     4921 gcgcgaacac cctgagttcc aaggccaagc agctgctgca ggaactgacc aagccgtaag
     4981 aaggtaaggg cgcaggtcgt ctggccagcc tacttctcgc cccacttgag cttcgaccgc
     5041 agcacgtcaa aaaagccgtt ctcgcccagc cggatcagct tcacgcaata atccgacttt
     5101 ctgcactgca gctcatctcc cacgcgcagt tcgacggcct cctggccatc gaccgtcagg
     5161 aaagtctgat ccggaatccc ctcgatccga accaggacgc gcgacgaccc ctggagcacc
     5221 atggggcgca gcgtcagttg atgcgggcaa atcggcgtca ccaccagaac atccacccgc
     5281 ggcaccatca ccggcccgcc cgcggccagc gaatacgccg tggatccggt cggcgtcgcc
     5341 acaatcactc catcggcgcg aaagctcgcc gccagctgct cgtcgacttc caccgcaaag
     5401 tggcccatcc gcgcgatcgc gcccttcgcc accaccacgt cgttcagcgc ctcatgctcg
     5461 ctgtagacct ttccgtcgcg ccagagctcg ctgtgcagca tcgctcgcga ttcgacacag
     5521 cagacgccct tcccccatgc ctccagatgc gcatagagat ccgtgagccg aacttcggtg
     5581 agaaacccca gcgatccaag gttcacgctg agaatcggca tgccgctgcg cgcgaacacc
     5641 cgggcggctg ccagcagcgt cccatctcct cccagaacga tcaccagatc cggattcagc
     5701 gaagccagct ccggacgcgg gtgcaccggc tcatccagca cataggttga actcaccgga
     5761 tccagcagag gctcgtgccc gtgcgctttc agccacagca ccagatccgg cagcaactgc
     5821 cgcagctcct ctttttgggg tttggagatg atggcgatcc gcgacatgcg tcctagtgta
     5881 tgacaggacc ccaggggcgc aggtcacagg tcgcaggtca cagacccccg gcacggtgcg
     5941 ccgcgacgcg acgactggct aacctgccga ctcgctccgt ttttccctgc gctctgtgcc
     6001 ctgtaccctg cgccctgttc ttacgtcgtc ggccggctct tcaccgccat gaactgcgcc
     6061 ggcactccgg tgccgcctgc gttcgtcagc ttcggatcca gcgtctgcgt gtccgtcagc
     6121 gtaccggtat tcaccatgtg cacctgattg tccccgctgg tgctgacaaa gaagactgcg
     6181 ccatccggac tgaagacgcc ggccaccgga gcctgggctc ctgaagagag ctgaaccgtg
     6241 ctcagcgtgc cctgtccacc ggtgttcagc ggctgatagt aaggaagggc gcccgtggcg
     6301 ccgctcgcgt tgtaggttac gaacgccacc gaggagtcgg gcgacgcaac cacctgatcg
     6361 atctcggtag cggtcacagg cagcgttgcc gtgatcggat tcgtcgtaac ggtcagcgcg
     6421 ggcccgttat acgccggaca ggcgttcacc ggaacaccgg ttggcgctcc ggaagaggag
     6481 cccagcagaa tatcggagaa cgtcgtgttg tccgcgccga gaacatggtt tccgtccgcg
     6541 gttgccgtga gctgcgtggt ggtcacgccc gcatccccgg ccggcgggaa ataaggcggc
     6601 gttgagctgc tgttcgggca gtaactgcgc gccgctgtcg aggttccgct caggaacgtc
     6661 gctacgctgg gcaccgtgat cgtcagcgcc ggtccgcaga acggatccca gttcgggtta
     6721 taggcgggcg ctgcggttgt ccctccggcc tccagcgcac agctatacgt cggctggttg
     6781 gtcagcggat agctggacca acccgtcgag ctgttgttca caaacatcgt gttttgccct
     6841 gtggctggat tctgccccac gatgtaaacc gttgtgccat cgggtgagta ttgggcgcgg
     6901 gatgcgattc ccgcaatgct ggtcaccgtt ccggtctgag cgccgtagag gtaaatcacc
     6961 tggcgaagct gatcgttgat gactaccgtg gacccggtgg gcgaaactgc cagcaccacg
     7021 cccggcactc ccgccacttc tttattgagg gtgttggtca gcgtgctgta ggtcatcagc
     7081 tcgtggtagc tgccgaagta cagcgtgtcc ccctgctgat ccatcaccat cgagttcggc
     7141 gtgtagggca gccggatggg agacgccgct cctccggtag tcaggtcgat ctcggaaaaa
     7201 tacggcgact gcgacgaggc aaaccagatg atgttgctgc tccgcccggg tgagttgacg
     7261 ttaacgcgat tgcccgtcac cggcatgccg ttgccgagat tgccaatcag attcaccggc
     7321 gcaggattgc acgtcggcgg ctggcaggtg gccgtgaccg tagcgtgact cgggaacgat
     7381 gcgctcacca gccccgtgct gctcacggaa agattctgcg gctcggtcga cgaataggtg
     7441 agagccagac cattgatcgg gttattgttc gaatcggtca ccgccgcaac caccgtctgt
     7501 ggagagcttg ggttcacggt cgccgacgtc gagccgttga tcgacagcgc gatcgtcttc
     7561 ggcgggcagg tggagaagaa tccggccgcg gaggtcactt ccgaggtcgc tgcgttgatc
     7621 accgtcgatc ccggcagatt cgccgtagcc gccccgttcg ggttcggctg gccggacgcg
     7681 ctgcccggcg ttgaactggt gggatcggtc gtgttgttga tgttcacgac atccttggtc
     7741 accgcgttgt aggtgatcgt tccaacgttc gccttcggat cggtgcagga aagcgtcgtt
     7801 gggctgcaca aggttgtgcc gccgggcccc gtaatcgtgg tctccgagag cagcgacgtt
     7861 tgcagcgtct gcccctggga caggcaggcg gtctgcgcag ggat
//
LOCUS       DASZYN010000815         4057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1156141, whole genome shotgun sequence.
ACCESSION   DASZYN010000815 DASZYN010000000
VERSION     DASZYN010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156141"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            520..2055
                     /locus_tag="VHE33_20440"
     CDS             520..2055
                     /locus_tag="VHE33_20440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99880.1"
                     /translation="MAADPQLAVSYTHVVVTARAAFAAYTKSGLLVSGPTNGKTFWVL
                     GGIPDHGSNWNIGDMRIVYDAYRQRFFMSTLYAGVDTNNKVLDGEQLLVAVSKSTDPT
                     QGWWIYSFDMPFHSTSPPPAWQSGDTCDYDTLGVGPTVYLMTANWFSGAGALKGSDIY
                     MFDANAMANGNSPAGWIFYNWTNPDGSPIATMQPAVHHGSTGSANTVWFATHWNGYAL
                     VYNHVDNPLKSNQAAWTTWGAMTATSLPLVTGPQKGVTNPTPPPMDMGNQVGDWFLKA
                     AWRNNRLYLTTTNTFLQNGVRLSAARVLRKNTSTAALEVDKTFGDSTTGTSQIHYGWA
                     AVEANANGDMIIPTSRTSSAMFPEIRVSQWLSGDSTIEPDFLLKSGEAPYHEGSVCIP
                     PSGGNPGLPWECWGETTGVSVDPTDDLGIWVTFEYPVPVPPGQNSNYPNHSLWVGQLG
                     GSGCGHSVCTTGQPIPQSCDGICVYNVCNQDSYCCNVAWDSICVSEVPTYCAGASCNN
                     QSP"
     gene            complement(3821..>4057)
                     /locus_tag="VHE33_20445"
     CDS             complement(3821..>4057)
                     /locus_tag="VHE33_20445"
                     /inference="COORDINATES: protein motif:HMM:NF025126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HVT99881.1"
                     /translation="RTTHLQTRPPSASSKAPATSVSQVIRKRVEEIFGWGKSVAGLRR
                     TRLKGRRKTWDNSYLVVAAYNLTRMVRLLRAAAA"
BASE COUNT          754 a         1208 c         1238 g          857 t
ORIGIN      
        1 gctaggggct gtgggctgcg ccccatcgtc cgttcgtgag ccaagctgca cgacggtacg
       61 ttcctctccc gcgacgctgg gtccagctcg cattccgtgc gcccataatt accagcccca
      121 ccatcgtcga atccacaagg agactcaatg agacgaccca gactcctagc aatgctcgtc
      181 gcgccgttcg ttctgtccgc aagcgctatc tcggcaccat ttgccgtggc ggctcctcaa
      241 ttgccgccag ccgacgtgcc aacgtcttcg tcgactggaa tggcggtgcc aaagagtaca
      301 atccatggcc cctacgagat aaccgccggt gccatggcta cgttttctca ctcgctcacg
      361 agcgttcctc ccccgctcaa tcgcgcgagt cgcccggcga cgtcgacacc actcgtctcc
      421 tccttggcac caacgggccc cggcctgaat aatagctggg aggcctatgg acccaactac
      481 aatacgggcg tgtcatatgg ggggttggcg atgggtggaa tggctgccga tccccagctg
      541 gcggttagct acactcacgt cgtagtgacg gcacgagctg ctttcgccgc gtacaccaaa
      601 tcagggttgc tggtctcggg tccaactaac ggaaagacgt tctgggtgct tggcggcatt
      661 cctgaccacg gttcaaattg gaacatcggc gatatgcgta tcgtttacga tgcgtatcgt
      721 cagcgattct tcatgtcgac gctgtacgcg ggcgtcgata ccaacaacaa ggttctggac
      781 ggcgagcagc ttctcgtcgc cgtgtcgaag agcaccgatc cgacccaggg atggtggatc
      841 tattcattcg acatgccttt tcactcaacg tcgccccctc ccgcctggca aagcggcgac
      901 acctgtgact acgacactct cggcgtgggt cctactgtgt atctgatgac tgcgaactgg
      961 ttcagtgggg ccggggcgct gaaagggtcc gacatttaca tgtttgatgc gaacgccatg
     1021 gccaacggga actcacccgc ggggtggatc ttctacaact ggaccaatcc cgacgggtct
     1081 ccgattgcta ctatgcaacc ggccgttcat cacggcagca cgggctcggc gaacactgta
     1141 tggttcgcca cgcactggaa tggctacgca ctggtctaca atcacgtaga caatccattg
     1201 aagtccaatc aagcggcatg gacgacgtgg ggagcaatga cggcaacctc gcttccgctt
     1261 gtgacgggtc ctcagaaggg cgtcactaac ccgactccgc cgccgatgga tatgggcaac
     1321 caagtcggcg actggttctt gaaagcggcg tggcggaata acaggctcta tctcactacc
     1381 acaaatacct tcctccagaa tggagtcaga ctttccgcag cccgcgttct ccgcaagaac
     1441 acctcgacgg ccgcgctgga agtcgataag acgttcggag actcgacgac cggcaccagt
     1501 cagatccact acggatgggc tgcggtcgaa gcgaatgcga acggagacat gatcatcccg
     1561 acgtcgcgaa cgagctcggc catgttcccc gagattcgcg tgtcgcagtg gctgagcggc
     1621 gactcgacga tcgaaccgga ctttctcttg aagagcggtg aggcgccgta ccatgaaggc
     1681 agcgtctgta tcccgcccag tggcgggaac cctgggttgc cttgggaatg ctggggggag
     1741 accactggcg tatcggtgga tccgactgac gacctaggaa tttgggtaac ctttgagtac
     1801 cccgtccccg ttccgcccgg ccagaacagc aactatccca accacagttt gtgggtcggg
     1861 cagcttgggg gaagcggatg cggtcacagt gtctgcacga ccgggcagcc aatcccgcag
     1921 agttgcgatg gaatatgcgt ctataatgtc tgcaatcagg attcctattg ttgtaacgta
     1981 gcgtgggaca gcatttgtgt cagcgaagtc ccgacatact gcgctggtgc gagctgcaat
     2041 aatcagtctc cctgacctcg acctgatgcg tcgccgcgcg agcattgctc gaacggcgac
     2101 gcaggcgcgc agtggataaa gctgccacgt ttgtctctag tcaccgaact agcgctcaac
     2161 aacgtatgcg tttaggcgcg attaacgttc tatcgtgact gttgcatgat aaaatcgagg
     2221 ccgtgagcac gcgcgggcaa aaggactatt tccgtgtcgt attcgcgact atcgctatta
     2281 catcctgcgg cggaagccat gaactgcagg ttggtactga ctcttcgata ccgcagaatg
     2341 cggacgcagg cgcgtccctc gacgcatcgc gcgacgcgcc acggttgggg tgcccagcgg
     2401 gcccgcctcc cgagacgggt gcggtgtgca atgtgacaac gcagctctgc tcttacgggg
     2461 acagcgatga tgcgctgtgc cggtcgatgt ttgtctgcga tgggtcttct ttcacgccct
     2521 ttaacgtagg cggtaggtgt ccgccagctt cctgtgcgtt gggaatgaag aacggcgatc
     2581 cttgcggtac tgaaggattt tcgtgtcgtg gtgagaatgg gacgacttgc tcgtgctcgt
     2641 cctgtatgcc gatgccggca ggatgtaccg gcagtccctg gtggcaatgt gaagcgcctc
     2701 cctcatcacc atgtccaacc acggcgccga acgccggatc accatgtgaa gctccgatgg
     2761 actgtcctta cgtgtctcag gcatcgggct cagttctgga ggtgtgcgcc aagtgtgatg
     2821 gttcgagctg gaactggttt ccgccagact atcaggctca taagtcctgc ccgacccaaa
     2881 aatgtgtcgg atcatgcttg gtcggagcgg cctgtccccc gggccggcca gtggttgttg
     2941 gagtctgccc gctcggcagc tcatgctgtg gcggtcctta gagatgggtc caggtttgaa
     3001 tcggaccccg tggtttcatc cgtgtcgggg caccggctcg ttgtgtattg cggcgcgtcc
     3061 cccggcgcgc ccgcgagcgg tgtagtagca gttctggcgt gagtgccctg gttcgtcggt
     3121 tgctatctgt cggagtaacg actatgggtc tgtttagtag ctgggtgaca attctggcgt
     3181 tcggctttgt cgggataggt tgcgacggac tcgcaggcga caggcagcca ctcatggcgg
     3241 gccccccaag cgtagtcaaa gagaagcgac ctgcgctcgc agttggcgga gcggcgccag
     3301 actctggcct cctccaggat gggggtctag acacgctgct ttcgtcggag gacctcgccg
     3361 gctcggaggt cagcggctca gccgccttgg caggaaccta ccaggcgatg tgccagcact
     3421 actgcgacac gcttgaaaag acagacctat acgcgtgcct ctcgatgaac gcgagtgatt
     3481 gcgccgcccg gttcagtacc gtcgccgccg agtgcgtcga cctccggtgc tcttcggagc
     3541 ttgttcagcc atcactctgc ctcactcagt gcgacgcttt ggcgacgaat ctttctgcat
     3601 attgcaggat agcccccgcg ggcgtcactg catgtgcatc gccgctggtg aagcagaatg
     3661 agacgtgtcg cgccggttgc aatgtctcgt ctccatagtc atcgggttct agcaggctgc
     3721 tgaaataggc cgtttccggc cgaagtagtt tgtgccgcgt gccggttcgg cgtcgctaag
     3781 cgaggcggtc cgcctcggga ggccgcctca ggcctccgcg tcaggcggcc gctgctcgta
     3841 gtagccgcac catccgcgtg aggttgtacg cggccacaac gaggtagctg ttgtcccagg
     3901 tcttccggcg gcccttcagc cgcgtgcgcc ggagaccggc cacggacttg ccccagccga
     3961 agatctcttc gacgcgtttg cgaatgacct ggctgactga cgtagccggc gctttggacg
     4021 aagcgctggg cgggcgagtt tgcaggtgcg ttgtccg
//
LOCUS       DASZYN010000816         3885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_255049, whole genome shotgun sequence.
ACCESSION   DASZYN010000816 DASZYN010000000
VERSION     DASZYN010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255049"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..292)
                     /locus_tag="VHE33_20450"
     CDS             complement(41..292)
                     /locus_tag="VHE33_20450"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HVT99882.1"
                     /translation="MLETRPPIAVTINGARLQVTPGTTVAAAVLMAGSHTRTSVRGEA
                     RGPLCGMGICFECRVTIDGVLHQRSCQILCATGMQVTVP"
     gene            complement(285..1427)
                     /locus_tag="VHE33_20455"
     CDS             complement(285..1427)
                     /locus_tag="VHE33_20455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HVT99883.1"
                     /translation="MNSAWDVLVLGAGIVGTACARECAALGLRTAVIDAAGVGGGATA
                     AGMGHIVVMDDSPAQLALTCWSQALWRELAADLPAEAEYEARGTLWVAADEEEMAEVR
                     RKHQVYATAGLATEILDSQVLADAEPQLRRELAGALLVPSDGVLYPPSAAGYLMREAI
                     RLGAKMLQPGRVIDAKQGRVRLEDGSEMQAGRIVIACGTSATQLLPELPIRKRRGHLV
                     ITDRYPGFVRHQLVELGYLKSAHSLTTDSVAFNVQPRQTGQLLIGSSREYGAEDSSIN
                     HPLLRAMIERARMYLPAVGNLHATRVWTGFRAATPDKLPLIGPWPGDDSLILATGHEG
                     LGITTSLATSKLVAAALTGDEPGIPPEPYFPARFAYGVAAAEVLHA"
     gene            complement(1424..2371)
                     /locus_tag="VHE33_20460"
     CDS             complement(1424..2371)
                     /locus_tag="VHE33_20460"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HVT99884.1"
                     /translation="MPVIETVDSHTAGEPTRVVVSGGPDLGRASLADRLRQFAAEHDA
                     FRSAVVNEPRGSDVLVGALLQEPTDPRCTAGVIFFNNVGYLGMCGHGTIGLVTTLAHL
                     GRIRPGEHWIETPVGVVRATLQSNGQVTVENVVSYRYRDAVPVSVPGYGIVYGNIAWG
                     GNWFYLLDAAPCDLVLENQEALTAYTWAIRGALTANRITGEGGAEIDHIEVSGPALTA
                     EGDSRNFVLCPGRAYDRSPCGTGTSAKLACLAAEGKLAPGKVWRQEGILGTIFEGSYR
                     LENGTPDRIVPSITGSAFITGESRLILDPQDPFCSGIRA"
     gene            2505..3242
                     /locus_tag="VHE33_20465"
     CDS             2505..3242
                     /locus_tag="VHE33_20465"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HVT99885.1"
                     /translation="MAVHVASSGKSSRRPKPEHGNSLRKAFHEIRELIVHGRLSPGSW
                     IVEADLARHLAMSRTPVRSALHLLQREGYVIEHTNVRKSRMVVAPLTQEDATELYQVI
                     GHVEGLAGRRTATLPKSRRNEIAARLKQFNEQLREIAESGDSRASGIFEIDRSFHRAI
                     IEAGAGPRLTALHKSIEPQAERYWRLYASWIMHDLHPSLEEHDEIISAISNGDADRTE
                     RALQVNWENGCARLAKVIEIFGERGSW"
     gene            3466..>3885
                     /locus_tag="VHE33_20470"
     CDS             3466..>3885
                     /locus_tag="VHE33_20470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99886.1"
                     /translation="MKKRLIVPTFFLAGIGVLPLAGNGQATRAEYDRALNLQKNYEGL
                     PIDLPDNVSWLPAGDRFIYRKSIEGGHEFVLVDAATQTKQPAFDHTKLAAALSSASGE
                     NYTAITLPFARFHFTDGDAGIEFAVENTRWQCDLHTWT"
BASE COUNT          755 a         1309 c         1079 g          742 t
ORIGIN      
        1 gccggtcctg cgccgacgac cagaatgtcg aatcttgtgt tcacggcacc gtcacctgca
       61 tgcccgttgc gcagagaatc tggcagctgc gctgatgcag aacgccatcg attgtcactc
      121 ggcactcaaa gcaaatcccc attccgcaga ggggcccgcg cgcttcacca cgaacggacg
      181 tccgcgtgtg agagccggcc atcaacaccg ctgccgcaac cgtggtccca ggcgtcacct
      241 gaagacgcgc gccgttgatc gtcaccgcaa tgggcggccg cgtttcaagc atgcaggacc
      301 tccgccgcgg ccacgccgta cgcaaagcgg gccgggaaat acggctccgg cgggatgcca
      361 ggctcatcgc cggtcagcgc ggctgccacc agcttgctcg tggccagtga ggtcgtgatg
      421 cccaggcctt cgtgtccggt cgcaaggatc agcgaatcgt cgcccggcca gggcccgatc
      481 agcggcagct tgtccggcgt ggccgcccgg aacccggtcc acacacgagt cgcatgcagg
      541 ttcccgaccg caggcaggta catccgcgcc cgctcgatca tcgctcgcag taacggatgg
      601 ttgatcgatg agtcctcggc cccgtattcc cgcgatgagc cgatcagcag ttgcccggtc
      661 tggcgcggtt gcacgttaaa cgccacggaa tccgttgtca gggagtgcgc gctcttgaga
      721 tatcccagct caaccagctg atggcgcacg aatcctgggt agcgatcggt gatcacgagg
      781 tgtccgcgcc gctttcggat cggcaattcc ggcaggagtt gcgtcgcgct ggtaccacag
      841 gcgatcacaa tgcgtcctgc ctgcatttcg cttccatctt ccagtcttac tcgtccttgt
      901 ttcgcatcga tcacccttcc cggttgcagc atctttgcgc cgagccggat cgcttcgcgc
      961 atcaggtatc ccgctgccga tggcgggtac aggaccccgt ccgagggcac caaaagcgct
     1021 cccgccagtt cccggcgcag ctgcggctcg gcgtctgcca gcacctggct gtcgagaatc
     1081 tccgtagcga gtccagccgt ggcatacacc tgatgcttgc gtcggacctc cgccatctct
     1141 tcctcatccg cggccaccca caacgtgcct cgcgcttcat attcagcttc cgccggcagg
     1201 tctgcggcca gttcacgcca aagagcctga gaccaacagg tcagcgcaag ctgcgccggg
     1261 gaatcatcca tgaccacgat gtgtcccatt cccgccgcgg tcgctcctcc tcccactccg
     1321 gcggcgtcaa tcactgccgt acgcagcccc agtgccgcgc actcgcgtgc acaggccgtg
     1381 cccacaatgc cggctcccag gaccaacaca tcccacgccg aattcatgcg cgtatgccgc
     1441 tgcagaacgg gtcctgagga tcgaggatca gccggctctc ccccgtaatg aaagcagaac
     1501 cggtaatgct ggggacgatg cggtccggtg tgccgttttc cagccggtaa gaaccttcaa
     1561 agatcgtgcc cagaatgcct tcctgacgcc acaccttccc cggagccagc ttcccttctg
     1621 ccgcaaggca ggccagcttc gcgctggtgc cggttccgca aggcgaacga tcgtacgccc
     1681 tgccaggaca caggacgaag ttgcggctgt ctccttcggc cgtgagcgcc ggaccggaga
     1741 cctcaatgtg atcgatttcc gccccgccct caccggtgat tcggttggcc gtcagcgcac
     1801 cgcggatcgc ccacgtgtag gcagtcagcg cctcctggtt ttccagcacc agatcgcagg
     1861 gcgcggcgtc cagcagataa aaccagttcc ccccccatgc aatatttccg taaacgattc
     1921 cgtagcctgg aacgctgact ggcaccgcat cccgatagcg atagctgacg acattctcga
     1981 ccgtcacctg cccgttggat tgcagagtcg cccgcacgac gcccactggc gtctcgatcc
     2041 agtgctctcc tggccggatt cgcccaaggt gggccagcgt ggtcacgagg ccgatggtgc
     2101 cgtggccgca catgcccagg tagccgacgt tattgaaaaa gatcactccc gccgtgcacc
     2161 ggggatccgt cggctcctgc agcagagctc ccacgagcac gtccgatccg cgcggctcat
     2221 tcaccactgc ggatcggaag gcgtcgtgct cagccgcgaa ctggcgcaga cgatcggcca
     2281 ggctagctcg tcccagatca gggccccctg aaaccactac gcgcgtgggt tctccggcag
     2341 tgtgagagtc aacggtttct atcacaggca taagtcgaaa gtgccagtat tgtctacaat
     2401 tctggatact accctattcc cgtgcgagcg cgttccgaat tcgctcgggg gtgacccagc
     2461 agcggggtaa aatcccttgg aaggtgcggg gaaacaattc tcttatggct gtgcacgtgg
     2521 catcgagtgg taaatcctcc aggcgtccaa aacctgaaca cggaaacagc ctgcgcaagg
     2581 cgttccacga aatccgtgag ctgatcgtac atggccgcct ctctcccgga tcctggatcg
     2641 ttgaagcgga tctcgccagg catctcgcca tgagccgcac tcccgttcgc agcgcgcttc
     2701 acctgctgca gcgcgagggc tatgtcatcg agcatacaaa cgtccgcaaa tcgcgcatgg
     2761 tggttgcgcc gctgacccag gaagacgcca ccgaactcta tcaggtcatc ggccacgtgg
     2821 aaggacttgc gggacgccgc acggctaccc ttcccaaatc ccgccgcaac gaaattgctg
     2881 ctcgactgaa gcaatttaac gagcagcttc gcgaaattgc agagtccggc gattctcgcg
     2941 cctccggcat cttcgaaatc gaccgcagct ttcaccgcgc catcatcgaa gctggcgccg
     3001 gtccgcggct caccgcactg cacaaatcca ttgaaccgca agcggagcgt tactggcggc
     3061 tttacgccag ctggattatg cacgatctac acccctccct cgaagagcac gacgagatta
     3121 tctccgcaat cagtaacggc gacgccgatc gcaccgaacg cgcgctgcag gttaactggg
     3181 aaaacggctg cgcacgtctt gccaaagtca tcgaaatctt tggcgaacga ggcagttggt
     3241 aatcgcaccc cagggttgat cgctccctgc tgaggcgacg gccttgactt gtcgcatgcg
     3301 gtattgtaca caatgctgac agcaatgatc ctctgattct ccgccccagc ggattgtgga
     3361 tttccggatc ctcgttccgt tcacttggaa ctccgtttcc taacgctttc ggactcactc
     3421 acgacactcg tcaccggcat gcaaatcaag gcgctcagga ggcacatgaa gaagcggttg
     3481 attgtcccca catttttcct cgcgggcatc ggtgttctcc ccctcgcggg taacggtcag
     3541 gcgactcgag cggaatacga tcgtgccctg aacctacaga agaactatga gggacttccc
     3601 atcgaccttc ccgacaacgt ctcctggtta cctgctggcg accgcttcat atatcgaaaa
     3661 tccattgaag gcgggcacga gttcgtactt gttgacgccg cgacccagac taaacagccg
     3721 gcatttgacc acaccaaact ggcagctgcg ctttcttctg ccagcggcga gaactacacg
     3781 gcgatcacac tgccctttgc gcgctttcat ttcactgacg gtgacgccgg catcgaattc
     3841 gccgttgaga acactcgctg gcaatgcgat ctgcacacct ggacc
//
LOCUS       DASZYN010000817         3196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_255059, whole genome shotgun sequence.
ACCESSION   DASZYN010000817 DASZYN010000000
VERSION     DASZYN010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255059"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="VHE33_20475"
     CDS             complement(<1..1103)
                     /locus_tag="VHE33_20475"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HVT99887.1"
                     /translation="MASIGLMLVRPRGLPEWVWIGGGAVLLVATRLLPVRGAAYAIRE
                     GVDVYLFLTGMMLLAELAREEGVFDWVADIAVHHARGSAGRLFLWVYGVGVVVTALLS
                     NDATAVVLTPAVLAVVRKAKVEPRPYLLACALIANAASFLLPIANPANLVVYGAELPP
                     LAVWMRMFLMPSVAAIVATFVCLRLLARRELGGQAAEVGERYRLKAAGWMALAGIGIA
                     AAGLLVASAKDVPLGAPTFGAGVVALMLVAMQDRSAPKNAVRNVAWSVLPLVAGLFMI
                     VEALNRAGMLRLTRAGLAWLAAAPDGVGKFCGGFGIALLSNAMNNLPAGLAAGTALRQ
                     MGQHGLLAHAVMIGVDLGPNLSVTGSLATILWL"
     gene            1261..2538
                     /locus_tag="VHE33_20480"
     CDS             1261..2538
                     /locus_tag="VHE33_20480"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HVT99888.1"
                     /translation="MPTPERLYYADSFLQSFPAVVADVRELSRAEGESVWQIALDRTA
                     FYPTSGGQPFDTGLLRAISPGGASLDIPIDSVEEDDNGQVWHFVRKPLSIGARVEGHI
                     DWERRFDHMQQHTGQHLLSAIFLSELNAPTVSFHLGDAVSTIDISGGPFAAHSLERIE
                     RLCNQIIAEDRPVSMRYVPRAEAESMLAAGELRKLPDRSGDIRLVEIDGIDRNACGGT
                     HLRSTGQIGGLLLRGTEKVSRGVRVSFVCGLRAVRAARADAGILAQLTSALSAAPADI
                     PAAVERLRSESKAIVKERQRLREELADYHAARLAVEVQIENRLRIVDRLWKDRDPEYC
                     RLLASRLTAAAPSTIVLFCTETTDPASIVLARSLDFSFDCGRILKEALSQLGLRGGGS
                     ADFAQGEVPPAQARALRTDLIAAVRKAVADERS"
BASE COUNT          628 a         1238 c          770 g          560 t
ORIGIN      
        1 atcagccaca ggatcgtcgc cagcgacccc gtcaccgaca aattcggccc caaatccacc
       61 ccgatcatca ccgcgtgcgc cagcagcccg tgttgcccca tctgccgcag cgccgtcccc
      121 gccgctaaac ccgccggcaa gttgttcatc gcattcgaca gcagcgctat tccgaatccg
      181 ccgcaaaatt tccccacccc atccggcgca gccgcgagcc acgcaagccc cgcccgcgtc
      241 agacgcaaca ttcccgcccg attcagcgcc tccacaatca tgaacaggcc cgcgaccagc
      301 ggcagcacgc tccacgctac gtttcgtacc gcattcttcg gagcgctccg atcctgcatc
      361 gctaccagca tcagcgctac caccccggcc ccaaacgtcg gcgcgcccag cggcacatcc
      421 ttcgccgacg ccaccagcaa ccctgcagcc gcaatgccaa ttcctgccag cgccatccat
      481 cctgccgcct tcagccgata tcgctcgccc acctccgctg cctgtccgcc cagctcccgc
      541 cgtgcaagca gccgcaggca aacaaacgtc gccacaatcg ccgccaccga cggcatcaga
      601 aacatccgca tccacacagc gaggggcggc aactccgccc catacaccac caggttcgcc
      661 ggattcgcaa tcggcagcaa aaaacttgcc gcattcgcga tcagcgcgca tgccagcaag
      721 tacggccgcg gctccacctt cgccttccgc accaccgcca gcacggccgg cgtcagcacc
      781 accgccgtcg cgtcgttcga caacagcgcc gtcaccacca cccccacgcc gtaaacccac
      841 aaaaacagcc gtcccgccga tccccgcgcg tgatgcaccg caatatccgc aacccagtcg
      901 aaaaccccct cctcgcgcgc cagctccgcc agcagcatca tcccggtcag gaacaggtac
      961 acatccacac cctcgcggat cgcatacgcc gccccgcgca ccggcagcag ccgcgtcgct
     1021 accagcaaca ccgcaccacc gccaatccac acccactccg gcagcccgcg cggacgcacc
     1081 agcatcagtc cgatgctcgc caccgcgatg atccaaatca gaacgcctga catttttgta
     1141 acgaatggag atgcgaatac ttcgagacag ttggatgccc tcgcattaca ctaaatccgg
     1201 tttaccccag acagttcctg ttatgatctg gcgcgcacga cgccagacgt ggagttcccg
     1261 atgcctacgc ccgaacggct gtactacgcc gattcgtttc ttcagtcatt ccccgccgtc
     1321 gttgcggacg tccgcgaact ttcacgcgca gaaggtgaat ccgtctggca aatcgccctc
     1381 gatcgcaccg ccttctatcc caccagcggc ggtcagcctt tcgacaccgg cctccttcgc
     1441 gccatctctc ccgggggcgc ttctctcgac atccccatcg actcggtcga agaagacgac
     1501 aacggccagg tctggcactt cgttcgcaag cctctctcca tcggcgccag agttgagggg
     1561 cacatcgact gggagcgccg cttcgatcac atgcagcagc acaccggcca gcatctgctc
     1621 tctgccatct ttctgagcga gctcaacgcc cctaccgtct ccttccacct cggcgacgcg
     1681 gtctccacca tcgacatttc cggcggaccc ttcgcagccc acagcctcga acgcattgag
     1741 cgcctttgca accagatcat tgctgaagac cgccccgttt ccatgcgcta cgttccccgc
     1801 gccgaagccg aatccatgct cgccgccggc gaactgcgca agcttcccga ccgctccgga
     1861 gacatccgcc tcgtcgagat cgacggcatc gatcgcaatg cctgtggtgg aacccacctt
     1921 cgctccaccg gccagatcgg cggcctcctg ctccgcggaa cagaaaaagt cagccgcggc
     1981 gttcgcgtca gctttgtctg cggcctccgt gccgttcgtg ctgcccgtgc cgatgccggt
     2041 atcctcgccc agctcacctc cgccctctcc gccgctcctg ccgacatccc tgccgccgtc
     2101 gagcgcctgc gatccgaatc caaagcaatc gtcaaagaac ggcagcgtct tcgcgaggaa
     2161 ctcgctgact accacgccgc gcgcctcgcc gtcgaagttc aaatcgaaaa ccgcctccgc
     2221 atcgtcgacc gcctctggaa ggaccgcgac cccgagtact gtcgcctgct ggcctcgcgc
     2281 ctcaccgccg ctgccccatc caccatcgtg ctcttctgca ccgaaaccac cgaccccgcc
     2341 tccatcgtcc tggcgcgcag ccttgatttc agcttcgact gcggccgcat tctcaaagag
     2401 gcgctctcgc agctcggcct gcgtggcgga ggctcggccg acttcgccca gggcgaagtc
     2461 ccacccgcgc aagcccgcgc tctgcggacg gatctcattg ccgctgtccg caaagcggtt
     2521 gctgacgaaa gaagctgacg aaggcccgca ctggaacctg ccgcagtacc ttcgtaccgt
     2581 tcgcctctcg gatttcgctt gcctccggcc tggcccagat cgcaaactgg aaacaaggaa
     2641 agctctgtcg cacaggcggc agagctttcg tgtttcagaa cgctggtctc gtcgcagcgc
     2701 attgggtcag cctgcgacct gtgacttgtg acctgtgacc tgtgatctgt actcgccccg
     2761 gctcaggccg gatcaggctg gctccttgaa actttgcgca tcacgcactc gcatccgcga
     2821 atgcagcact cacaaccgtg aatgcagcac cgagaaccgc gaatgtcgca ctgacaactg
     2881 cgaatgcagc accgacgctc gcgaagcaac tgccatcgat gcaatcattt gcctgtctgg
     2941 cggttgtctt tgccgcattc acaagcgcga atacagtatt cccatcgcga tgcaactgcc
     3001 ctttcctccg gaagatagca tgttgtcaaa cttgtaaact cgcgtcaaac tcttaaatct
     3061 caattatttg cgtctacggc gagttgcaga aacacaagtt caatttccat gcgctccgga
     3121 tgcacgactc ctgcggatcg cggtcctctg ttaaccactt cgttatccat gagggggtag
     3181 gcccaaagcg cctctc
//
LOCUS       DASZYN010000818         3250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_182472, whole genome shotgun sequence.
ACCESSION   DASZYN010000818 DASZYN010000000
VERSION     DASZYN010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182472"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VHE33_20485"
     CDS             complement(<1..330)
                     /locus_tag="VHE33_20485"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HVT99889.1"
                     /translation="MPSITTIRVRYAETDQMGVAYYANYFIWCEIGRVELLRQLGFEY
                     KQMELEHNCHLPVVEASCRYKSPARYDDLITIETRVSGLRTSVIKFSYRFLATRLEAP
                     APILLAEA"
     gene            complement(482..955)
                     /locus_tag="VHE33_20490"
     CDS             complement(482..955)
                     /locus_tag="VHE33_20490"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HVT99890.1"
                     /translation="MVLVRTWPVIVLAALLTTAPAFATRTHRAATSGHSSHHKSKKHH
                     RFVPGQRGIEPERAKEIQDALIREHYLTAPASGQWDNDTEAAMQKYQADHGWQTKLTP
                     DSRALIKLGLGPQTDTGSHPPAATPAAGSAVPSAANNDSSHAAAGTLAAISTIHN"
     gene            complement(1093..2763)
                     /locus_tag="VHE33_20495"
     CDS             complement(1093..2763)
                     /locus_tag="VHE33_20495"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HVT99891.1"
                     /translation="MKFLPGRFRYNRLASTFTILATLSAGILAGSFLAHKVRGQESQV
                     NSSDATPLKVPAPHEMPTDFSRIAKEIGPAVVNINTQILPKQQQQQRRRGPHYMQPWS
                     APQQQQQPPDGDDQDQGPDGQGGQGGQGGLQDFFNRFFGGQGPDMGPGEDGQERESLG
                     SGFIVDPRGYIITNNHVVDKADRVFVKLASDPEGNQGRPATVVGVDKATDIAVLKIQT
                     DTPLPTIKVGNSDGVQVGDWVMAIGEPFGLAKTVTAGIISSKNRTVEPGTAGEFQHFL
                     QTDAAINPGNSGGPLVNMDGQVIGINTAIFTQSAGNEGIGFAMPSNTVVDVYNDLIGP
                     EHKVVRGSIGISFQPNLPSAVARVYGFKSGVIISQITPGQPAAKAGLKVGDIITTLDG
                     KPVKDGDELVAVISARKPGSTIEVGYLRDGQNLHATLSIADRDKMMAAINAASSEEGQ
                     EGSNRTPGTISPAKLGITVQDLPQGAPAGLHGVLIQSVKPGSFADEIGVGNFQGGVIV
                     SLNRHAVHNVQEFQTIVAGLKPGDDVALEIVDPQKPNGGTSILGGTLR"
BASE COUNT          591 a          858 c         1154 g          647 t
ORIGIN      
        1 cgcctcggcg agaaggatcg gcgcaggggc ttcgaggcga gtcgcgagaa agcggtagga
       61 gaacttgatc accgacgtgc gcagtccgga gacgcgggtt tcgatggtga tcaggtcgtc
      121 atagcgcgcg ggcgatttgt aacggcagga agcttcgacg acggggaggt ggcagttgtg
      181 ttccagctcc atttgtttgt actcgaagcc aagctggcgg aggagttcga cacggccgat
      241 ttcgcaccag ataaagtagt tggcgtagta ggcgacgccc atctggtcgg tctccgcgta
      301 gcggacgcgg atggtggtga tggatggcat gaggtcaggt tagcgaagaa gcggtcagct
      361 agaaagcggt cagctaacaa gcggccagct aaagaccggt cagctaagga acggtcagca
      421 aaaagctgtt cctcaggagc ggtcagtggc gcccacttct gctgaaccgc aagtcggata
      481 cttagttgtg gatggtgctg atggcggcca gggttccggc ggccgcgtgg gacgaatcgt
      541 tgttcgctgc tgagggaacc gcagaaccgg cagcgggtgt tgctgcgggt ggatgactgc
      601 ccgtgtcggt ctgggggccg agaccgagct tgatcagggc tcgggagtcc ggcgtgagct
      661 tggtctgcca gccgtgatcg gcctggtact tctgcatggc cgcctcggtg tcgttatccc
      721 actggccgga agcgggagcc gtcaggtaat gctcacggat gagcgcatcc tggatttcct
      781 tcgcgcgctc gggctcaatg ccccgctgtc cggggacgaa gcgatggtgc ttcttcgact
      841 tgtggtgcga ggaatggccg gaggtcgcag cccggtgggt ccgcgtagcg aacgcggggg
      901 cggtggtcag gagcgcagcg agaacaatga ctggccaggt tctgacaaga accatgcaac
      961 aggtacctca gaagtaaaca gttcggcaat ctatgggtca gggtaaaagg gaccgaccca
     1021 aattgcaaca gaaactgcag gggctcccga aactttggtg ggggagcccc tgttgtcagg
     1081 tgggatgcca ggttaccgca gcgtgccgcc caggatcgaa gttccgccat tgggtttctg
     1141 cggatccacg atttcgaggg ctacgtcgtc ccccggcttc aggccggcca cgatggtctg
     1201 gaattcctgg acgttgtgga ccgcgtgacg gttgagcgaa acaatgacgc caccctggaa
     1261 gttgccgacg ccgatctcat cggcgaagga gccaggtttc acggactgga tgagaacgcc
     1321 gtggagtccg gcaggagcgc cctggggcag atcctgaacc gtgatcccaa gtttggcggg
     1381 tgagatggtg ccgggcgtgc ggttcgagcc ctcctgaccc tcctcgctgc tggcggcatt
     1441 gatggcggcc atcatcttgt cgcggtcggc gatggacaga gtagcgtgca ggttctggcc
     1501 gtcgcggaga taacccacct cgatcgtgga accgggttta cgagccgaga taacagcgac
     1561 cagttcgtcg ccatccttca ccggcttgcc gtcgagcgtg gtgatgatgt cgccgacctt
     1621 gaggccggcc ttggctgcgg gttgacccgg ggtgatctgg ctgatgatca cgccggattt
     1681 gaatccgtaa acacgggcga ccgcgctggg cagattgggc tggaagctga tcccgatact
     1741 gccgcggacg accttgtgct ccggaccgat gaggtcgttg tagacatcga cgacggtgtt
     1801 ggagggcatg gcaaagccga tgccttcgtt gccggcggac tgggtgaaga ttgcggtgtt
     1861 gatgccgatg acctgaccat ccatattgac cagaggcccg ccggagttgc cagggttgat
     1921 ggctgcgtcg gtttgcagga agtgctgaaa ttctccggca gtgccgggct cgacggtgcg
     1981 gttcttgctg gagatgatgc cggcagtgac ggtcttggcg aggccaaagg gctcgccgat
     2041 ggccatgacc cagtcgccga cctggacgcc gtcggagttg ccgactttga tggtgggcag
     2101 cggagtgtcg gtctggatct tgagcacggc gatgtcggtg gccttgtcga cgccgacgac
     2161 cgtggcggga cggccctgat tgccttccgg atcggaggcc agcttcacga agacgcgatc
     2221 ggccttgtcg acaacgtggt tgttggtaat gatgtagccg cggggatcga cgatgaagcc
     2281 ggaaccgagg gattcccgtt cctggccgtc ctcgccgggg cccatatccg gtccctgacc
     2341 gccgaagaag cggttgaaga agtcctgcag gccaccctgt ccaccctggc ccccctggcc
     2401 gtcgggtccc tgatcctgat cgtcgccatc gggcggctgc tgctgctgtt gcggtgcaga
     2461 ccacggctgc atatagtgcg gcccccggcg gcgctgctgt tgctgctgtt tgggcaggat
     2521 ctgggtattg atattgacca ccgcggggcc aatttctttg gcaatgcggc tgaagtccgt
     2581 gggcatttcg tggggtgcag gaactttaag gggcgtggcg tcggagctgt tgacctggga
     2641 ctcctgtccg cgaaccttgt gggccaggaa agaaccggcc aggatgcctg cggagagggt
     2701 ggccaggatc gtgaaggtcg aagccagacg gttgtagcgg aaccgccccg gaagaaattt
     2761 cattcggatc gtacctcgta gtcgtttgcg gtcggcgcgg atgcgtcgtc ccacgatcag
     2821 tatagaaccc gtaaaacacg ccatgcgcgc gccgcggggc gcaggctgaa gcgttttcct
     2881 ttagacgctt gaccgtctgc cggggttaca ggatgggatg cgtgcacagt ccaccggttg
     2941 tgggacgccg gaaatgcagt tgggagttgt gagtggaagg cgagttggag tggctggcag
     3001 gggagaagcc accaaaatgc ggtcgatcga acatatccga ttcgctttcc gggctaaatg
     3061 cctttgctca aagagcttcg atttcgcttg aaccaacagt cgttacggcg ctcagttgct
     3121 cagtgggacg ccctcgtggc tgctgggctg gaccggcggg ggaagcaatt cggtgagggc
     3181 aatgccggcg aggatcagca gggcaccggt agcgctgcgc tgattgaggc gttctccggc
     3241 gaagagaaag
//
LOCUS       DASZYN010000819         4641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_693275, whole genome shotgun sequence.
ACCESSION   DASZYN010000819 DASZYN010000000
VERSION     DASZYN010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693275"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VHE33_20500"
     CDS             complement(<1..434)
                     /locus_tag="VHE33_20500"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HVT99892.1"
                     /translation="MSATTNLLNPYAKFLNGQEPLAVLPNTPGKLKALAQKIGPGRMT
                     TPPAPGKWSPRDILCHLADCEIAFAFRLRQTLAEDHHTVQPFDQDRWSTPYSGIDANE
                     ALATFSLLRRWNVLLIEAALPQNAAKPVKHPERGEMTFSTIV"
     gene            681..1283
                     /gene="rimP"
                     /locus_tag="VHE33_20505"
     CDS             681..1283
                     /gene="rimP"
                     /locus_tag="VHE33_20505"
                     /inference="COORDINATES: protein motif:HMM:NF036577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HVT99893.1"
                     /translation="MAFDVDQIRATAQRVAASHGLDVVEIEFHGGAKHRILRVFIEKN
                     AEERKKLADAADAAGLQTALAAGTGVDQLAGVTHTDCEGFSRDFGAVLDVEDLIPGAE
                     YTLEVSSPGLDRKLYGIDDYRRFHGSLVRLQTFEPVAGNRHWQGRLGEVTDTLLKLVM
                     QKKAAGKKPSGRAQGAAKPESNEVEIALKNIEKAQLVPEI"
     gene            1348..3042
                     /gene="nusA"
                     /locus_tag="VHE33_20510"
     CDS             1348..3042
                     /gene="nusA"
                     /locus_tag="VHE33_20510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HVT99894.1"
                     /translation="MASVLYESIEALSRDKGIDPQIVITAVEDAIALATRKYYKTQEN
                     MRAELDKDTGEIRAYVFKTVVQDEDQLEDPLNQITLEEAKELAPEVEVGGEIRYYKPT
                     DVLGRIAAQMAKQVIFQKVREAERDTVFNEYAHRVGEVLNATVKRLELQDVIFDLGKA
                     EARMPRREQSRLEQFAVGERVRVVLQRVDKAARGPQVIVSRAAPELVQNLFQSEVPEI
                     YDNTVTIRAIAREAGERTKIAVMSRDKDVDPVGACVGMKGMRVQSIIRELRGEKIDII
                     EFSEEITTFAEKALQPAKVSRVSITDLTDKQLEVIVDDTQLSLAIGKKGQNVRLAAKL
                     LGWKIDIKSEEEKRQEVEQQMTAMSGATATPIEQVSELGESIIQKLVTAGITTVESLA
                     DMTPEQLEEIPGIGEKTLEKISVAVRHYFGQYEEGEERPALAAAGAEGAQASEDAEVA
                     AARKAEEASTDEEIDALRQDLTQSGEEPVSEDGSGLITDDVAEERLAELTEVGPDLDS
                     EGAESVDPGQEDTSETLAAHKPDPEIARDEAVVEGNVEEVKEEELGDKKVDEGTGA"
     gene            3269..>4641
                     /locus_tag="VHE33_20515"
     CDS             3269..>4641
                     /locus_tag="VHE33_20515"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99895.1"
                     /translation="MDALTAVGVTEKKTHSSSIETDEAERVRTHILRGRGGSSSSASS
                     RHEADVKPKIDWSQAHKPGDIARQILERKQQEAHAAAAPRPPAPAVTPAGPVVVRPAP
                     GVPAAVSAPAAPKPAPAAAPATPAAPAAAAPVPAEPKPAPRKIVPAPRQAPQIVTAPP
                     AASSTRTGVVAAPPPPTPAIAAKPPAGPVVAKPPVAAQSPAASAAPGEGRPAAGPIVA
                     ARPSAAAPTPVSAAPAAVPGPVVAAASAAPPVSEAVELTRAAAPAVEPDRPSAPPAPP
                     PRRVIMPQTGPRPVYSAPPPPPAAAPGPGGGPGGPGGIQRGRPIFDRRPTGGPGGPGG
                     PRPGMGGGPGGPGARRPMHPTRSAPGGPGGPGQRPGMGARPGFGARPGFGQRPGMGGA
                     PGLPPPGEAPRPARPGPRGRGRPRYEKSKEGPMKGFAPPSRMAAQVPTGPVPITRTIT
                     VTEGVSV"
BASE COUNT          926 a         1554 c         1436 g          725 t
ORIGIN      
        1 tccacaatgg tcgaaaacgt catctcgccc cgctccggat gcttcaccgg cttcgctgcg
       61 ttctgcggca gcgctgcctc gatcagcagc acattccacc gccgcagcag cgaaaaggtc
      121 gccagcgctt cattggcgtc gatgcccgag taaggcgtcg accacctgtc ctggtcgaag
      181 ggctgcaccg tgtgatgatc ttccgccagc gtctgccgca gtcgaaatgc gaaggcaatc
      241 tcacagtcgg ccagatggca caggatgtcc cgcggcgacc acttcccagg cgcgggcggc
      301 gtcgtcatgc ggcctggccc gatcttctgg gccagcgcct tcagcttccc cggcgtattc
      361 ggcagcacag ccagcggttc ctggccattc agaaattttg cgtacgggtt gaggaggttc
      421 gttgtggcgg acattcgtat cctctttccg gagcagtatt ccacgggcgg ctgaggaagg
      481 gggaagcgag tgacagacgc gtgcaaagcc cgatttgatc cagcctcgca acccagttac
      541 tataggtaca gacgagtttg tccggctgcg cggagtttcc ggccggggaa atgagtgggc
      601 gcgagcccac tttttatttg ctcatttttt ggcattcgat gttgagcaac aactatggca
      661 ttcgatgttg agcagggttt atggcatttg acgtcgatca gattcgcgcc accgctcagc
      721 gcgtcgcggc ctcccatggc ctcgatgtcg tcgagatcga gtttcacggc ggagcgaaac
      781 accgtattct gcgcgtcttt atcgagaaga acgcggagga gaggaagaaa ctcgccgacg
      841 ccgccgatgc cgctggcctc caaaccgccc tggccgctgg taccggcgtc gatcagctgg
      901 ccggcgtcac gcacaccgac tgcgaggggt ttagccgtga ctttggcgcc gtcctcgacg
      961 tcgaagacct gattccgggc gctgaatata ccctcgaggt ctcttcgcca ggactcgatc
     1021 gcaagctgta cgggattgac gactaccggc gctttcacgg atcgctggtc cggctccaga
     1081 ccttcgagcc ggtcgcaggc aacaggcact ggcagggaag actcggagaa gtgaccgaca
     1141 ccttgctgaa gctggtcatg cagaagaaag ccgcgggaaa gaagccctcc ggccgggccc
     1201 agggcgcagc gaaacccgag tcgaacgaag ttgaaattgc gctgaagaat atcgagaagg
     1261 cgcagctggt tcccgaaatt tgaaagacag tgccccgggc gcaggctcag agcacgaaga
     1321 aacaatcagg actgagagaa gaacaagatg gcgagcgtgc tttacgagag cattgaagca
     1381 ttgagcaggg ataagggcat cgacccccag atcgtgatca ccgcggtcga ggatgcgatc
     1441 gcgctggcga cccgcaagta ttacaagacc caggaaaaca tgcgtgccga gctggataag
     1501 gacaccggcg agattcgcgc ctacgtcttc aagaccgtcg ttcaggacga ggatcagctc
     1561 gaagacccgc tgaaccagat caccctggaa gaagccaagg aactggctcc cgaagtcgaa
     1621 gtcggcggcg agatccgcta ctacaagccc accgatgttc tgggccgcat cgccgcccag
     1681 atggccaagc aggtcatctt ccagaaagtg cgcgaggctg agcgcgatac cgtcttcaac
     1741 gagtacgccc accgcgtcgg cgaagtcctc aatgccaccg tcaagcgcct tgagctgcag
     1801 gacgtcatct tcgacctcgg caaggccgag gcccgcatgc cccgccgcga gcagtcccgc
     1861 ctcgagcagt tcgccgtcgg cgaacgcgtg cgcgtggtgc tgcagcgcgt cgacaaagcc
     1921 gcccgcggac cccaggtcat tgtctcccgc gccgcccccg agctggtgca gaacctcttc
     1981 cagagcgaag tgccggagat ctacgacaac accgtcacca tccgcgccat cgcccgcgaa
     2041 gccggcgagc gcaccaagat cgccgtcatg tcccgcgata aggatgtcga cccggtcggc
     2101 gcctgtgtcg gcatgaaggg catgcgcgtc cagtccatca tccgcgagct gcgcggcgaa
     2161 aaaatcgaca tcatcgagtt cagcgaagag atcaccacct tcgctgaaaa agccctgcag
     2221 ccggccaaag tcagtcgcgt ctcgatcacc gacctcaccg ataagcaact ggaagtcatc
     2281 gtcgacgaca cccagctatc gctcgccatc ggcaaaaagg gccagaacgt ccgcctcgcg
     2341 gccaagctgc tgggctggaa aatcgacatc aagagcgagg aagagaagcg ccaggaagtc
     2401 gaacagcaga tgactgctat gtcgggcgcc accgcaacgc caatcgaaca ggtaagcgaa
     2461 ctcggcgaat cgatcatcca aaaacttgtg acggccggaa tcaccaccgt ggagtccctg
     2521 gccgacatga cccccgaaca gctggaagag attccaggca tcggggagaa aactttggag
     2581 aagatcagcg tggccgtgcg tcactatttt ggacaatacg aagaaggcga ggagcggccg
     2641 gcgctggccg ccgcaggcgc tgagggtgcg caagcctccg aagacgccga ggtcgccgcc
     2701 gcccgcaaag cggaagaagc ctcgaccgac gaggagatcg acgcccttcg tcaggatctg
     2761 acccagagcg gcgaagaacc ggtcagcgag gacggttccg gcctcatcac tgacgacgtc
     2821 gccgaggagc ggctggccga actcaccgag gtcgggcccg atctcgacag cgaaggcgcc
     2881 gaatcggtcg atcccgggca ggaagatacc agcgaaaccc tcgccgccca caaacctgat
     2941 cccgaaatcg cccgggatga ggccgtcgtc gaaggcaatg ttgaggaagt gaaggaagaa
     3001 gagctgggcg acaagaaagt tgacgagggt acgggcgcat aacccgctgc aggcgcccgt
     3061 tcggcgcgga tttgtcggcc caattcaccg gaattgagtc gaacccgcgc gttttcgggg
     3121 ataatgatag agatagccga cccacaagtc aggagttgtc cgtatgaaga actaaggacg
     3181 gtgggagttt ttttgaacac ggggacggat gagcacagtt cgcatcaatg aattagcccg
     3241 agagctcgaa gtcaagagca aggaaatttt ggacgctctc acggccgttg gagttaccga
     3301 gaagaaaacg cattcgagct cgattgagac cgatgaagcc gagcgtgtgc gcacccatat
     3361 tctcagaggg cgcggcggca gctccagctc tgcatcgtcg cgtcacgaag ccgacgtcaa
     3421 gccgaagatc gactggtcgc aggcgcacaa gcctggcgat atcgccagac agattctcga
     3481 gcgaaagcag caggaagccc atgctgccgc cgcgccgcgt ccgcccgcgc cggccgtcac
     3541 tcccgcagga cctgttgtcg tgcggcccgc tcccggagta cccgctgcgg tcagcgctcc
     3601 cgccgctcct aaacccgcgc ccgcggccgc gcccgcgaca ccggccgcac ctgctgccgc
     3661 cgctccggtt ccggctgaac cgaagcccgc gcccagaaaa attgtcccgg ccccgcgtca
     3721 ggcgccgcag atcgtcaccg cgcctcctgc cgccagttcc acgcgcaccg gcgtcgtcgc
     3781 cgctcctcct ccgccgactc ccgcgattgc ggcgaagccg cccgctggtc cggtcgtcgc
     3841 gaagccgccg gttgccgccc aatccccggc cgcatccgct gcgcctggcg aaggacgtcc
     3901 ggctgcaggc cccatcgtcg ccgcccggcc ctcggccgct gcgccaacac ctgtctcagc
     3961 agcgcccgcc gctgtgcccg gccctgtcgt tgccgcggca tccgctgcgc ctccggtttc
     4021 tgaagccgtc gaactgacca gagccgccgc accggcagtc gaacccgacc ggccctcggc
     4081 tcccccggct ccgccgcctc gtcgcgtcat catgccgcag accggtcccc gtcccgtcta
     4141 cagcgcgccg ccgcccccgc ctgctgcagc ccccggaccc ggtggtggcc ccggtggtcc
     4201 cggcggtatc cagcgcggcc gccccatctt cgatcgcagg cccacaggcg gccccggcgg
     4261 ccccggtggt ccccgtcccg gcatgggtgg cggtcccggc ggtcccggtg cacgccgtcc
     4321 catgcatccc acgcgctctg cacccggcgg tcctggtgga ccaggccagc gtcccggcat
     4381 gggtgcgcgc cccggattcg gcgcacgtcc cggatttggc cagcgtcccg gcatgggtgg
     4441 cgcgccaggc ttgccgcctc cgggcgaggc cccgcgtccg gcacgtcccg gtccgcgtgg
     4501 ccgcggacgt ccgcgttacg agaagagcaa agaaggcccg atgaagggct ttgcgccgcc
     4561 gtcccgcatg gcagcgcagg ttcccaccgg ccctgtcccc atcacgcgca ccatcaccgt
     4621 gaccgaaggc gtcagcgtga a
//
LOCUS       DASZYN010000820         6478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1083625, whole genome shotgun sequence.
ACCESSION   DASZYN010000820 DASZYN010000000
VERSION     DASZYN010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083625"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VHE33_20520"
     CDS             <1..644
                     /locus_tag="VHE33_20520"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HVT99896.1"
                     /translation="AYAHVRGGGDLGKAWHDAGRLMEKRNTFTDFIAATEHLVAQGWG
                     AKGRVAAEGGSAGGLLMGAVANLRPDLYRAIISHVPFVDVMNTMLDPSLPLTVPEYEE
                     WGDPNQKEAFEYMLSYSPYDNLEAKEYPAMLVKTSLHDSQVMYWEPAKYVAKMRTLRT
                     DKRNLVLHTNLTAGHGGASGRYDYLKEIALDYVFLMRELDVIADQTWAAKNAS"
     gene            complement(614..1891)
                     /locus_tag="VHE33_20525"
     CDS             complement(614..1891)
                     /locus_tag="VHE33_20525"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HVT99897.1"
                     /translation="MPEESLRRSSLWMISGQGIAMAAQAVYFVLIGRALGSREYGAFV
                     GVAALIAALSQFSSLGMEMILVRNVSRDRDSFARTWGHALAITAAGFVLLFVAATLYA
                     HFALRPELRPLVPWIALADGLLGKLIQLAARAFQGAASLAWTARLTAFTNIARAATAA
                     LVLAWARAHATHATALLWTRVYWLSTLFVALFALTIITLRLGFPRFRPIRRRDLSEGL
                     SFSVSSSSISVYNDIDKTFLVSLGQISAAGIYSAAYRVVDAASAPIYAVYAAAAPRFF
                     REGAHGVHRARDFSRKTLSRTLPYSVAVAVLLALSAPLLPFLFGPSFRGSVAALRWLC
                     VLPVLRSLHYAWGSAITASASQWNRTATQFGAGALNLCLNAVLIPRWSWQGAAAASLL
                     TDGALAAASFLVLSRLIRREVAAAQDAFFAAQV"
     gene            complement(1899..3032)
                     /locus_tag="VHE33_20530"
     CDS             complement(1899..3032)
                     /locus_tag="VHE33_20530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HVT99898.1"
                     /translation="MPNVLIYKSDLLHPSETFVAAQAHALRRFTPCFVGLRRIPTSLL
                     IDPCETITLTQNDTLRDRLHRRFFLETGIAPDFLRALQRSQPVLIHAHFAVDGAAALP
                     LQKQLRLPLIVTLHGYDATSTDAFQRTWAPGRVYLRRRDELRARTRFFVCVSEHIRQQ
                     AIERGVAPDKIRVIPIGVDLGFFAPDPLRSRDLDPVVLFVGRLVEKKGCTHLISAMAQ
                     VEARHPTAKLLIVGDGPLLDPLRAQAHAQLRRCTFLGSQPPSVVRDLMYRASVLGVPS
                     IIAADGDTEGLPITLCEAQAIGLPIAAFDGPGVSEAVVASETALLVSQGDEGGLAEAI
                     SRILDDPRVRIRLARAGRLRAEENFSLEKQTARLEDLYCEALQ"
     gene            complement(3032..4438)
                     /locus_tag="VHE33_20535"
     CDS             complement(3032..4438)
                     /locus_tag="VHE33_20535"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase"
                     /protein_id="HVT99899.1"
                     /translation="MMLSLLHTRPPHKSTTIPLEDLICVLVFAFFAMQGAIPFIAPAQ
                     SLEMTGSAPTPLTTLGGIASQAVVNILILALLLRRPRYFIRRVADFPWILLPALLAIL
                     AIASAAWSLDPFITLRRSLPFALAGLFGFWFATRYSLVRQVAILRFAMLALSIATVLI
                     VLFDPALGLDHSPGHAVDWQGIFTQKNACGRMMVLATATILCAPARTLLRPTHFATLG
                     LFFFVLFMTGSRGAWMIETAVLVLFALFAFARHSGQRVRLILAATIPLAAIGLAAFGI
                     LDFRQLAPLFGRDPSLTGRTAIWAQVTHFIGERPCFGYGYDAFWRGMQGPSLQIAAAV
                     HFIVAHAHNGFLEIALELGIAGLVLFALSWLRGWFALWSLWQRGAIARIAWPLAILIL
                     ILLYDIDENTLLIYNGLFWVLYVSALVNIERARKSVWTRIQPLGDPHHTAPFAPHEIL
                     ESDPRFAPDIAVPVQEIS"
     gene            complement(4435..5427)
                     /locus_tag="VHE33_20540"
     CDS             complement(4435..5427)
                     /locus_tag="VHE33_20540"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HVT99900.1"
                     /translation="MRTPECIAWFSVWLMLLAAPAKIAAQQSASPVLSPLPPASEQPN
                     GPAPLAAPAQLPANPESLLIGPGDLLRISFLREPELDQHLRVLDSGEIVLTLAGKVSV
                     QQLTPAQAAARIATQYREGNFLLHPEVSVFVEEYATQAVTILGQVVHPGTVRLTAPRT
                     LIDVISLAGGLTPDADRHIVLQRGGKEGERIRTFLSNRAEDALNADILVRPGDTILVP
                     RAGIVYVLGDVARPGGYVMQNDSQLTVLQAVALAAGASKTASEKRVRLVRTIDGLSHS
                     IDLPLRDMERGRAPDVPLRANDILYVPFSLTKNISLGIANITAAASSALIYAIK"
     gene            complement(5414..>6478)
                     /locus_tag="VHE33_20545"
     CDS             complement(5414..>6478)
                     /locus_tag="VHE33_20545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99901.1"
                     /translation="VALRLARQEAGESRALYSSLLGRLQQTGVLEGLHSANFAVVSPA
                     LIPPSGHPASPNLTWLTLLALGTGTILGCGAAITRELTDDTLHTAAEFEALLDAPVFA
                     SVPAYRIDDPWYRRLLPPPPGSTVNLQAAADTDFETPPRETPFVEALHRLRASLLLSH
                     SDRAPQIITLTDPCHRPANPVRTASQGAPPSLGLSLATILAQHGSSVLFVDADLRSAP
                     ETSTSADPGLSELLSDKALPPLAHMIAGLPSLSVLHAGARPPCPAELIASSRMAALLA
                     EWREEFQFIVIRGPAAFHADALVLAQLSDAVLLAAHAGRTRRDEILPSFQELSRQIPN
                     HAVLGLVLQQVHPGPSYAHA"
BASE COUNT         1381 a         1597 c         2530 g          970 t
ORIGIN      
        1 tggcgtatgc gcatgtgcgg ggcggcggcg atttgggcaa ggcgtggcac gatgccggcc
       61 ggctgatgga gaagagaaat acgttcacgg atttcattgc ggcgaccgag catctggtgg
      121 cgcagggttg gggtgcgaag ggtcgcgtgg ctgcggaagg cgggagcgcc ggcggcctgc
      181 tgatgggggc ggtggcgaat ctgcggccgg atctgtatcg cgcgatcatc tcgcacgtcc
      241 ccttcgtgga cgtgatgaac acgatgctgg atccgtcgtt gcctctgacg gtgccggagt
      301 acgaggagtg gggcgatccg aatcagaaag aggctttcga gtacatgctg agctactcgc
      361 cgtacgacaa cctcgaagcg aaggagtatc cagcgatgct ggtaaagaca tcgctgcacg
      421 attcgcaggt gatgtactgg gaaccggcaa agtatgtggc gaagatgcgg acgctcagaa
      481 ccgataagag aaacctggtg ctgcatacga atctgaccgc cgggcacggc ggggcgtcgg
      541 gacgctacga ctacctgaaa gagattgcgc tggactacgt gttcctgatg cgggaactgg
      601 acgtgatcgc ggatcagacc tgggctgcga aaaacgcatc ctgagcggct gcaacctctc
      661 gacgaatcag acgcgagagg acaaggaagc tcgcggctgc cagtgcgcca tcggtgagga
      721 ggcttgcagc ggcagctccc tgccacgacc agcgcggaat caggaccgca ttcagacata
      781 aattgagagc gcctgcgccg aactgcgtgg cggtgcgatt ccactgcgag gcagaggcgg
      841 tgattgccga tccccaggca tagtggagcg agcgcaggac ggggagaaca cagagccagc
      901 gcagggccgc gacagatccg cgaaatgacg ggccgaagag aaacggcagc agaggcgcgg
      961 atagcgccag caggacggcc acggcaacgg aatacggcag cgtgcgggag agggttttgc
     1021 gcgagaagtc gcgggcccga tgaacgccgt gtgcaccttc gcggaaaaac cggggagcgg
     1081 cggcagcgta gacggcatag atcggcgcac tggccgcgtc gacgacgcga taggcggcgg
     1141 aatagatgcc ggctgcggag atctggccga ggctgacgag gaaggtcttg tcgatgtcgt
     1201 tgtagacgga aatggaggaa ctggatacgg aaaagctgag gccctcgctg aggtcgcggc
     1261 gacggatcgg ccggaatcgg ggaaagccga ggcggagagt gatgatggtg agggcgaaga
     1321 gagcgacgaa cagagtggaa agccagtaga cgcgggtcca cagcagggca gttgcgtggg
     1381 tggcatgagc ccgagcccac gcgaggacga gggccgcggt ggcggcgcgg gcgatgttgg
     1441 tgaatgcggt gaggcgcgcg gtccacgcga gagacgccgc gccctgaaac gcacgagcgg
     1501 ccagttgaat gagtttgccg agcaggccgt cggcgagcgc gatccatggg acgagtgggc
     1561 ggagttcggg gcgcagtgca aaatgggcat agagagtggc cgcgacgaaa agcaggacaa
     1621 agccagctgc tgtgatcgcg agggcatggc cccaggtacg ggcgaaagag tcccggtcgc
     1681 ggctgacgtt gcggacgaga atcatctcca tgcccagtga actgaactgg ctgagcgcgg
     1741 cgatgagggc cgcgacgccg acgaatgcgc cgtattcccg actgccgaga gcgcgcccga
     1801 tcaggacaaa atagacggcc tgggcggcca tggcgatgcc ctgtcccgag atcatccaga
     1861 gagagcttcg gcgaagggat tcttcgggca tggcgccgct actggagcgc ttcgcagtag
     1921 agatcttcga ggcgagccgt ttgcttttcg agactgaagt tttcctcggc gcggaggcgt
     1981 ccggcgcgag ccaggcggat gcgcacgcgg ggatcgtcaa ggatgcggga gatggcttcg
     2041 gcgagaccgc cctcgtcacc ctgggatacg agcagggccg tctcactggc tacgaccgcc
     2101 tcggaaacgc cggggccgtc aaaggcggca atgggcaggc cgatggcctg ggcttcgcaa
     2161 agcgtgatgg gcagaccttc ggtgtcgccg tcggctgcga tgatactggg cacgccgaga
     2221 acactggcac gatacatgag gtcgcggacg acgcttgggg gctgcgatcc gaggaaggtg
     2281 caacggcgca gctgtgcatg ggcttgcgca cggagaggat cgagcaatgg accgtcgcca
     2341 acgatgagca gttttgcggt gggatggcgt gcttcgacct gcgccatggc gctgatcagg
     2401 tgcgtgcagc ccttcttttc aacgagccgt ccgacgaaca ggacgacggg gtcgagatcg
     2461 cgcgagcgga gcgggtcggg cgcgaagaaa ccgaggtcaa ctccgatggg aatcacacgg
     2521 attttatctg gcgccacgcc ccgctcgatg gcctgttggc ggatgtgctc agaaacgcag
     2581 acgaagaacc gagttcgtgc ccgaagttcg tcgcgacggc ggagatagac gcggccagga
     2641 gcccaggtgc gctggaatgc atcggtggat gtggcgtcgt agccgtgcag ggtcacgatg
     2701 aggggaagac gcaactgttt ctgcagaggc agcgcggcag caccgtcgac cgcgaagtgc
     2761 gcgtggatga gaaccggctg ggagcgctgg agggcgcgca ggaaatcggg tgcgatgccg
     2821 gtttcgagaa aaaaacgacg atgaagcctg tcgcgaaggg tgtcgttctg cgtaagagtg
     2881 atcgtttcgc agggatcgat caggaggctg gtgggaatgc gccgcagtcc gacgaagcag
     2941 ggagtgaaac ggcggagggc atgggcctgc gcggcgacga aggtttctga agggtggagc
     3001 aggtccgact tatagataag aacgttcggc attacgagat ttcctgaacg ggcacggcga
     3061 tatcgggtgc gaatcgtggg tcggattcaa ggatttcatg gggagcaaag ggcgcggtgt
     3121 gatgagggtc acccagcggc tggatcctcg tccagacaga cttgcgggcg cgttcaatat
     3181 tgacgagcgc agagacataa aggacccaga agaggccgtt gtagatgagg agagtgtttt
     3241 cgtcaatgtc gtacaggagg atgaggatca ggatggcgag cggccaggcg atgcgggcga
     3301 tcgcgccgcg ctgccagaga gaccagaggg cgaaccagcc gcgcagccag ctgagtgcga
     3361 agagaacgag tcctgcgata ccgagttcga gcgcgatttc aagaaagccg ttgtgtgcgt
     3421 gggccacgat gaagtggacg gcggcggcga tctgtaagga aggtccctgc atgccgcgcc
     3481 agaaggcgtc gtagccgtat ccgaagcagg ggcgctcccc aatgaagtgt gtgacctggg
     3541 cccagatggc ggtgcgcccg gtaagggagg ggtcgcggcc gaaaagcggc gcaagctggc
     3601 ggaaatcgag gatgccgaaa gcagcgagcc cgattgcggc gaggggaatc gttgcagcca
     3661 ggatgagtcg gacacgctga ccggagtggc gggcgaaggc gaacagcgcg aacaaaacga
     3721 gcacggcggt ttcgatcatc cacgcgccgc gagagccagt catgaaaagg acaaagaaaa
     3781 acagaccgag ggtcgcgaag tgagtggggc ggagcagagt gcgggccggc gcacagagga
     3841 tcgtcgcggt ggcaagcacc atcatgcgcc cgcaggcgtt tttctgggtg aagatccctt
     3901 gccagtcgac agcgtggccg ggggagtggt cgaggccgag agccggatca aaaaggacaa
     3961 tcaggacggt ggcgatgctg agggcgagca tcgcgaagcg aaggatggcg acctggcgta
     4021 cgagagagta gcgggtggcg aaccagaatc cgaagaggcc ggccagggca aagggcaggg
     4081 aacggcgcag ggtaatgaag ggatcaagcg accatgcggc cgaagcgatg gcgaggatcg
     4141 cgagcaatgc agggagaagg atccagggga agtcggcaac gcggcgaatg aagtagcggg
     4201 gccggcgcag gagaagagcg aggatgagga tattcacgac agcctgggag gcgatcccgc
     4261 cgagggtggt gagcggggtc ggcgccgagc cggtcatttc gagggattga gccggcgcga
     4321 tgaaggggat ggcgccctgc atcgcgaaga acgcgaaaac gagaacgcag atgaggtctt
     4381 cgagaggaat cgtcgtggac ttgtgagggg gtcgggtgtg gaggagagac agcatcattt
     4441 aatggcgtag ataagagcgg agctggccgc cgcggtaatg ttagcgatgc cgagcgagat
     4501 gttttttgtg aggctgaagg gcacgtacag aatgtcattg gcgcggagcg gcacgtcggg
     4561 ggcgcgcccg cgttccatat cgcggagagg cagatcgatg gagtggctga ggccgtcgat
     4621 ggtgcgcaca agacgcacgc gcttttcact ggcggttttg gaggcgccgg cggcgagagc
     4681 gacggcctgg agcacggtga gctgcgagtc gttctgcatg acatagccgc cggggcgggc
     4741 cacgtcgccg aggacgtaga cgatgccagc cctgggaacg aggatggtgt cgccggggcg
     4801 aacgagaatg tcggcgttga gagcgtcttc tgcgcgattg gagaggaagg tgcgaatgcg
     4861 ttcgccctct ttgcccccgc gctgaagaac gatgtggcgg tcggcgtcgg gggtgagtcc
     4921 gccggcgagc gaaataacgt cgatgagcgt gcgcggagcg gtgagacgca cggtcccggg
     4981 atgcacgacc tggcccagaa tcgtcaccgc ttgtgtggcg tattcctcga cgaagacgga
     5041 gacctcgggg tgaaggagaa aattgccctc gcggtactgc gtggcgatgc gtgcggcggc
     5101 ctgggcaggg gtgagttgct ggacggagac ttttccggcc agggtgagca cgatctctcc
     5161 ggagtcaagg acacgaaggt gctgatccag ttcaggttcg cgcagaaagg agatgcggag
     5221 gagatcgccg ggaccgatga gcaacgattc cggattggcg gggagttggg ctggggcggc
     5281 gaggggcgcc ggtccattgg gttgctcaga ggccgggggg agcggagaaa ggacgggaga
     5341 cgcggattgt tgcgcggcga tctttgcggg ggcagcaagg agcatgagcc agacagagaa
     5401 ccatgcaatg cattcaggcg tgcgcatacg agggccctgg atgaacctgt tgaagaacga
     5461 gaccgaggac ggcgtggttg ggaatctggc ggctgagttc ctggaaggaa ggcaggattt
     5521 catcacggcg ggttcggcca gcgtgcgctg cgagcagcac tgcgtcggaa agctgggcga
     5581 gaaccagcgc gtcggcgtgg aaggcggcgg gaccgcgaat cacgatgaac tggaattctt
     5641 cacgccactc ggccagcaga gcggccatgc gcgaggaggc gatcagttct gcgggacagg
     5701 gtggccgtgc gccggcatgg aggacggaga gggagggcag accggcgatc atgtgcgcga
     5761 gaggcggcaa ggctttgtcg gagagcagtt cgctgaggcc aggatcggcc gaggtgcttg
     5821 tctcgggcgc ggaacgaaga tcggcatcaa cgaacagcac ggatgagcca tgctgggcca
     5881 ggattgtagc gaggctcaga ccgagggaag gcggcgcacc ctgcgaggcg gtgcgaacgg
     5941 gatttgccgg gcggtggcag ggatcggtga gggtgatgat ttgcggtgcg cgatcgctgt
     6001 gcgagagcag gagggaagca cgcaggcggt ggagcgcttc gacgaacggg gtttctcggg
     6061 gtggagtctc gaaatcggtg tcagcggccg cctggaggtt cacagttgag ccagggggag
     6121 gaggcaggag ccggcgatac cagggatcat cgatgcgata agcggggaca gaggcgaaga
     6181 cgggggcatc cagcagggct tcgaactcgg ccgcggtgtg aagggtatcg tcggtgagtt
     6241 cgcgtgtgat ggcggcgccg catccgagga tcgttccggt tccgagagcc agaagcgtga
     6301 gccaggtaag gttggggctg gcagggtggc ctgatggagg aataagagcc ggactgacga
     6361 cggcaaagtt ggcggaatga agcccttcaa gaacgccggt ctgctggagg cggccgagga
     6421 ggctgctgta aagcgcacgg ctctcgccgg cttcctgacg ggcgaggcgc agcgcaac
//
LOCUS       DASZYN010000821         3101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1083681, whole genome shotgun sequence.
ACCESSION   DASZYN010000821 DASZYN010000000
VERSION     DASZYN010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083681"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VHE33_20550"
     CDS             <1..831
                     /locus_tag="VHE33_20550"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVT99902.1"
                     /translation="DYRDISPGYFQTMRIPLLQGRSFAENDDARHPRVVVIDQTLARE
                     YFGNENPIGRHIVLPGGTRIPREVVGVVGAVHDSTLTGTPEATLYFPYMQGPDQSMSM
                     VVRSALPQNTILPEIRSAIESVDRDQPVFDVRSMPEIMGNVTSAPRIAFILLNILALV
                     ALVLAAVGIYGVTSYNVGQRTQEVGVRLTFGAPPHAILAMLVRQATTLMLLGVAAGVL
                     VGLLMTRLMSSLLYGVSSTDPVIFVGAAALVAMVATLACYLPARRVVRVDPLAALRAE
                     "
     gene            complement(1137..2612)
                     /gene="glgA"
                     /locus_tag="VHE33_20555"
     CDS             complement(1137..2612)
                     /gene="glgA"
                     /locus_tag="VHE33_20555"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HVT99903.1"
                     /translation="MHIVYAASECAPWARTGGLGEVLRALPRQIAAMGHKVSVFIPYY
                     RQVREFAHAQSQSSDKNYAVRSITIPFLGYSRFASILDGGKQDGVQMYFVDCPELFDR
                     EFLYGSPAGEYQDNWERYALFCRAVVEATKVLGAPDVYHVHDWQTALLPVYLRTLYEF
                     DPVLRNRGTVLTVHNAGYQGRWSPATVERLLFPWTLFTIDRAEFYGQFNYLKAGLVFA
                     DRLTTVSPRYAEEIQTHEYGQQLDGVFRQRAADLRGILNGLDYNDWNPAHDGNIAAHY
                     NPENLAGKADCRRDLLHAFGVPPQDDSTAVLGTVTRLTTQKGIDILAQVAEPLSRENV
                     VLLALGTGEEYYENLMRSLAAKYPGKVYTRIGYDVMLAHKVEAGADMFLMPSRYEPCG
                     LGQLCALKYGAVPIVRATGGLDSTVEEWNPAARTGNGFKFQGLEPEEFLAAIQRALTV
                     FRDDKEAWHTLMHNGMTEDHSWTTPAGEYLKVYEQVVTSRN"
     gene            2725..>3101
                     /locus_tag="VHE33_20560"
     CDS             2725..>3101
                     /locus_tag="VHE33_20560"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HVT99904.1"
                     /translation="MSATAQAAIVDALGDVSARTVVEIGPGRGAITALLAGRARRLVA
                     VELDRELAPRLREQFEGRGSVSILEEDVLKTDFAALRAGEAEKLLVVGNLPYYITSEI
                     LLRLFRYAEDVERAVVMVQREVA"
BASE COUNT          539 a          786 c         1158 g          618 t
ORIGIN      
        1 gattatcggg atatcagccc ggggtacttt caaaccatgc gcattccttt gctgcagggc
       61 aggagctttg cggagaacga tgacgcccgg catccgcgcg tggttgttat cgatcagaca
      121 ctcgctcgcg aatactttgg aaacgaaaac cctatcggaa ggcacatcgt tcttccggga
      181 gggacccgca ttccccgtga ggtggttggg gttgtgggag cagtgcacga ttcaacgctg
      241 accgggacgc cggaggctac cctctacttc ccgtatatgc agggcccgga ccaatccatg
      301 agcatggtgg tgcgctcggc gctcccgcaa aacacgattc tgcctgaaat tcgaagcgcg
      361 atcgaatccg tggatcgcga ccaaccggtg ttcgacgtcc gatcgatgcc agagatcatg
      421 ggcaatgtca cttcggctcc gcggattgcc tttatcctgc tgaacatttt ggctctggtg
      481 gcgctggtgc tggcggcggt tggaatctac ggcgtgacct cgtataacgt cggccagcgc
      541 acgcaggagg taggagtgcg actgacgttt ggcgctcctc cacatgcgat cctcgcgatg
      601 ctggttcgtc aggcaacaac gctgatgctg ctgggtgtcg cggctggggt gctggtcgga
      661 ttgctcatga cgcggctcat gtcgagcctt ctttatggag tgagcagtac ggatccggtc
      721 atcttcgtgg gagcggccgc gctggttgcg atggtcgcga cccttgcgtg ttatcttccg
      781 gcgcgccgcg tggtgcgggt ggacccgctg gcagccctgc gagcggagtg agggcgggcg
      841 ttttactgat gggggcggca ctcgttcagc acgcgcgtcg ctgggatcag agccgactgt
      901 gtgaattttt gccctgagag caaagcgcgc cgggcctaaa ggcgtgcctc ttcgaagacg
      961 tgatttcgcc gggttgaaac gcggcgcttt tgccgtcggt tgaggagaag aggtcaacga
     1021 agagaaagcc tcaggggctg aagcccaatc atttttgcgc cttttcggca cgactgaagt
     1081 catgccctgt tacaaaaggg ccggccaaat ccttcaacga actgcgggac accacactag
     1141 tttctgctgg tcactacttg ctcgtagacc ttgaggtatt cgccggctgg cgtggtccag
     1201 gagtgatcct cggtcatgcc gttgtgcatg agggtgtgcc aggcttcctt atcgtctcga
     1261 aagacggtga gggcgcgctg gatggctgcc aggaactcct ccggttcgag gccctggaat
     1321 ttgaagccat tgccggtgcg tgcggcgggg ttccactctt cgaccgttga gtcgaggcca
     1381 ccggtggcgc gcacgatggg gacagcaccg tatttgagag cgcacagctg gccgagtccg
     1441 cagggctcgt agcgcgaggg catgaggaac atgtccgccc cggcttcgac cttatgcgcg
     1501 agcatgacgt cgtagccgat gcgcgtgtag accttgccgg gatactttgc ggcgagggag
     1561 cgcatgaggt tctcgtagta ctcctcgccg gtgccgaggg cgaggaggac gacattttcg
     1621 cgcgacagcg gctcggcgac ctgggcgaga atgtcgatgc ccttctgggt ggtgagccgg
     1681 gtgacggtac cgaggacggc ggtggaatcg tcctgaggcg ggaccccaaa ggcgtggagc
     1741 aggtcacggc ggcagtcggc tttgccggcg aggttttcgg ggttgtagtg cgcggcgata
     1801 ttgccgtcgt gggcggggtt ccagtcgtta tagtcgaggc cgttgaggat gccgcggagg
     1861 tcggcggcgc gctggcggaa gacgccgtcg agctgctggc cgtattcgtg ggtctggatc
     1921 tcctcggcgt agcgggggct gacggtggtg aggcggtcgg caaagacaag gccggccttg
     1981 aggtagttga actggccgta gaactcggct cgatcgatgg tgaagagggt ccagggaaag
     2041 aggagacgct ccacggtcgc cggggaccag cgtccctgat agccggcgtt gtggacggtg
     2101 aggacggtac cgcgattgcg gaggacagga tcgaattcgt agagagtacg caggtagacg
     2161 ggcagcaggg ctgtttgcca gtcgtggaca tggtagacgt cgggggcgcc gagaactttg
     2221 gtggcttcga cgacggcacg gcagaagagg gcgtaacgct cccagttatc ctggtattcg
     2281 ccggcgggcg agccgtagag gaactcgcgg tcgaagagct cggggcagtc gacgaagtac
     2341 atctggacgc cgtcctgttt accgccgtcg aggatggagg cgaagcggct gtatccgagg
     2401 aaagggatgg tgatgctgcg gacggcgtag ttcttgtcgg aggactgcga ctgggcgtgc
     2461 gcgaattcgc gaacctgacg gtagtagggg atgaagacgg agactttgtg gcccatggcg
     2521 gcgatctggc gcgggagggc gcggagaact tcgccgaggc cgccggttcg ggcccagggc
     2581 gcacattcag aagcggcgta gacgatgtgc atggctttta cagggtacag gttgcaggtt
     2641 cgaggtgaca gagtgcgggt cggtgcacaa tcgatagaga tgcgcgagag aacgcaacgg
     2701 cccaggctgg ggcagaattt tttggtgagc gcgacggcgc aagcagcgat tgtggatgcg
     2761 ctgggggatg tgtccgcgcg gacggtggtg gagattggcc cgggccgcgg agcgatcacg
     2821 gcgctgctgg cgggccgagc gcgccggctg gtggctgtgg agctggatcg ggagctggcg
     2881 ccgcggctgc gcgagcagtt cgaggggcgc gggtcggttt cgattcttga ggaagacgtg
     2941 ctgaagacgg atttcgctgc gctgcgggcg ggagaggcgg agaagctgct ggtggtgggg
     3001 aatctgccgt actacattac gtcggagatt ctgctgcggc tgtttcggta tgcggaggat
     3061 gtggagcgtg cggtggtgat ggtgcagcgc gaggtggcgg a
//
LOCUS       DASZYN010000822         1710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_255886, whole genome shotgun sequence.
ACCESSION   DASZYN010000822 DASZYN010000000
VERSION     DASZYN010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255886"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1318)
                     /locus_tag="VHE33_20565"
     CDS             complement(11..1318)
                     /locus_tag="VHE33_20565"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HVT99905.1"
                     /translation="MAEGTPGETAYGKIVGGEDCRVASQPKNRAVERLLRSQSPLTAD
                     VETPYLRIFKSNVYVRDLDRSLPFYVDQLGFSVLADARLEFGRWVAVAPPDGSAILAI
                     VAPRKGTENYRLIGRHTNVSFIAEDINATYELWRGRGVRFLQPPQPQFWGGTFATFAD
                     LDNNVFELISSDEISREIEEQRRAASERTEAERRHAQELEIAKQVQARLFPQRAPALR
                     TLDYAGVCMQARHVGGDYYDFLSFGPHRVGLLVADISGKGIAAALLMANLQANLRSQL
                     ALAGECPATFLKTVNQTFFENTVDSAYSTVFFAEYDDETRVMRYANCGHLPGLLLRAD
                     GDVEHLCPTGTVLGMFREWDGHMGECRLGAGDVLALYTDGVTEACDAAEKEFGQKGLV
                     RALKRRRKLDAKGTVDGVLNELQKYSAGEQGDDITLIVARCTG"
     gene            1438..>1710
                     /locus_tag="VHE33_20570"
     CDS             1438..>1710
                     /locus_tag="VHE33_20570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99906.1"
                     /translation="MRKLLSLTLVALALLLAAPVSQAQTGSAGNTLVLVFKDGHRQRV
                     NLNDLQRLEFPGPVPAGIISTPGPSRARFLGRWEVGEGNGSNFFITL"
BASE COUNT          305 a          662 c          433 g          310 t
ORIGIN      
        1 agcgcgcctt ctaccccgtg caccgcgcca cgatcagcgt gatgtcatct ccctgctctc
       61 cggcgctgta cttctgcagc tcgttcagga ccccatcgac agtccccttc gcatccagct
      121 tcctgcgccg cttcagcgcc ctcaccagcc ccttttgtcc gaactccttc tctgcggcat
      181 cgcacgcctc cgtcacgccg tcggtgtaga gtgccagcac atccccggcc cccagccggc
      241 actctcccat gtgcccatcc cactcccgaa acatccccag caccgtgccc gtcgggcaaa
      301 gatgctccac gtcaccgtcg gcccgcaaca gcagccccgg cagatgccca cagttcgcgt
      361 agcgcatcac acgcgtctca tcgtcgtatt cagcaaagaa taccgtcgaa tacgcgctgt
      421 ccaccgtgtt ctcgaagaac gtctgattca ccgttttcaa aaacgtcgcc ggacactccc
      481 cggccagcgc cagctggctc cgcaaattcg cctgcaggtt cgccatcaat aacgcagcgg
      541 caatcccttt gccggaaata tccgccacca gcagccccac ccgatgcggc ccgaacgaaa
      601 gaaagtcgta gtagtcgccg cccacgtgcc gcgcctgcat gcacacaccg gcatagtcca
      661 gcgtccgcag cgctggagcc ctctgcggaa acagccgcgc ctgcacctgc ttggcaatct
      721 ccagctcctg cgcgtgccgc cgctccgctt ccgttcgctc gctcgccgcc ctccgctgct
      781 cttcgatttc gcggctgatc tcatccgagc tgatcagttc gaacacgtta ttgtcgaggt
      841 ccgcgaaggt tgcaaacgtc ccgccccaga actgcggctg cggaggctgc aggaaccgca
      901 cgccccggcc gcgccaaagc tcataggtgg cgttgatgtc ctctgcgatg aagctcacgt
      961 tcgtatgccg cccgatcagc cggtaattct ccgtgccctt gcgcggagcc acgatcgcca
     1021 gaatcgcgct cccatccggc ggagctaccg ccacccaccg cccgaactcc agccgcgcat
     1081 cggccagcac cgaaaacccg agctgatcga catagaaagg aaggctccgg tccagatcgc
     1141 gcacgtacac gttcgacttg aaaattcgca ggtacggagt ctcgacgtcc gccgtcagcg
     1201 gcgactggct gcgcagcaaa cgctccaccg cccgattttt cggctgactc gccacccggc
     1261 agtcctctcc gccaacaatc ttcccatagg ccgtctcccc tggtgtaccc tcggccacgg
     1321 aacggttcga gccgtttttg cgtaaagaaa actgcgcggg ctctacgccc ccgcgtgttc
     1381 cgtaccagtc ttgtttcggc aaacggcggg aaacgaagcg ttccgaagga gaaagccatg
     1441 cggaagctcc tcagcctgac ccttgttgcc cttgccctcc tgttggcggc ccctgtttcc
     1501 caggcccaga ccggttccgc gggcaacacc ctcgtcctcg tcttcaagga cggccaccgc
     1561 cagcgcgtga acctcaacga cctccagcgc ctcgaatttc ccggccccgt gcccgccggc
     1621 atcatcagca cccccggccc ttcgcgcgcc cgcttcctcg gcaggtggga agtcggcgag
     1681 ggcaacggca gcaacttctt catcaccctg
//
LOCUS       DASZYN010000823         5188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_630546, whole genome shotgun sequence.
ACCESSION   DASZYN010000823 DASZYN010000000
VERSION     DASZYN010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630546"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VHE33_20575"
     CDS             <1..495
                     /locus_tag="VHE33_20575"
                     /inference="COORDINATES: protein motif:HMM:NF015665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HVT99907.1"
                     /translation="HCIPIDPFYLSWKAKEFDFRTKFIELAGEVNLAMPYFVVESVME
                     AMSQQGKALRDSRILILGMAYKRDIDDLRESPSLTIIELLQKRGAQVSYNDPFFPTVG
                     HGRKYALNMTSTPLEKVGEFDCVVIVTDHSQYDYADIVSKAKLVVDTRNATRGIEAKN
                     LVRC"
     gene            complement(765..4802)
                     /locus_tag="VHE33_20580"
     CDS             complement(765..4802)
                     /locus_tag="VHE33_20580"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HVT99908.1"
                     /translation="MTLCVLPLALSGCGSIESNAATVGTLGASTGTLAFGNVTVGQTA
                     SNTITLKNDSVAAVQITQISVAGQSFALTSQTAVPMTLAAAGSLTLNLQFNPTAAGAA
                     SGKLTVVSNAASGTVTVSLTGTGTTAVTTPPPPTPVPPTVSAIACSSSTITGAGTDNC
                     TVTLSAAVDSSGGTVNLSSNDSAAVVPATVAVAPNMSSASFTVTAAAVSAAQTAIITA
                     AMGTSSATFNLQLNPLPSSGGTPAMSLSASALSFGSITVNTQATPQFVTVLSTGTAPL
                     TVSAATASGAGFSTAGPGFPITLNPGSTVTLQVNFTPANTGTATGSLTVTSDAPTGGT
                     AVVALSGNGTAPVPTGTPAMSLSASTLSFGDVTVNDQATPQFVTVISTGTAPLTVSAA
                     TASGAGFSVSGPALPITLNPGNAFTLQVNFSPTSAGAATGSLTVASDAPTGGTAVVAL
                     NGTGDAAAGVLNGLFCDSSTITGAGTDNCTVSLTAAAPAGGLAVSLSSSNSALTVPSG
                     VTVAAGNTNAFFTATATAVTSNATANVTASAGGASKIYAVRLLAQNPALSASTTTLNF
                     GDVNLNTTTTQPVTLTSTGSSAVTINSGTLAGAGFSMTGATFPLTLNPGQTATLQVSF
                     DPTTAGAANGSITIAGNASNGTIVISLSGNGQSAAGVLSSLSCTNQTLTGAATDPCSV
                     TLSAAAPIGGQVVTLSSNSSSVTVPGSVTVPAGSTSASFNATVSAVTSSTSATLSAAS
                     GGSTKVFTLQLNAQTMVLSVSTTNLAFGDVTVGTPASQPVTLTSSGTSPVTINTAALT
                     GTGFSMTGANPPVTLNPGQTVTLQVTFNPTTPGAATGSILITTNATTNPTANITLSGT
                     GDAAAGALSSLTCATSSYTAAGTDACTVTLGAAAGTGGLAVTLASNNSSVTVPASVTV
                     PAGATTATFNATVAAVSSTQTATLTATASGVAKTFAIQLSGAPGLTVSSTTLAFGNVN
                     LNTPTNKSVTLTSSGTAPLTISAATLTGTGFSMTGATFPVTLNSGQTATLQVTFNPTV
                     AGAATGSIAITSNAATNPNPAIALTGTGVAPVGVLSSLTCATSSYNAAGTDACSVTLS
                     AAAGTGGFAVTLASSNGAVTVPASVTVPAGSTTASFNAAVTAVTSTQTATLTASAGGV
                     VKTFAIQLTNGAPGLTLSSTTVAFGNVNLNTPSTQTVTLRSSGGSAVTISAATITGTG
                     FTMSGLTLPLTLNPGQTAVLNLQFDPTTAGAATGSVTITSNATTGATQTIALTGTGVN
                     SATYEVQLSWTAPASSADPVVSYNIYRSTGAGTYQKLNTAVNTPTSYTDTTVQSGVTY
                     NYEVTSVDGSGVESTPSNVYTAVIP"
BASE COUNT          869 a         1466 c         1812 g         1041 t
ORIGIN      
        1 cactgcattc cgatcgaccc gttctatctt tcctggaagg caaaggagtt cgacttccgc
       61 accaagttca ttgagctggc cggcgaagtc aacctggcca tgccttactt cgtcgtcgag
      121 agcgtgatgg aggccatgag ccagcaggga aaagcactgc gggactcccg catcctgatt
      181 ctcggaatgg cctacaagcg cgacatcgac gatctgcgcg aatcgccttc gctcacgatc
      241 attgagctgc tgcagaagcg cggcgcgcag gtctcttaca acgatccttt cttcccgacg
      301 gttggccatg ggcgcaagta cgcgctgaac atgaccagca cgccgctgga gaaggttgga
      361 gaattcgact gcgtggtgat cgtgacggac cactcgcagt acgactacgc cgacatcgtc
      421 tcgaaggcga agctggtggt ggatacgcgc aacgccaccc gcggcatcga agcgaagaac
      481 ctggttcgct gctagattgc tgtcccgaac attctctgaa aagttgagga tgagagggga
      541 acgaagagtg cttcaggggc tgaagcccac atgattctcg cgcctttgcg gcacgactga
      601 agtcatgccc tgttacaaaa gcgccggcca gttcttcaac gaacttgcgg gacaccacac
      661 cagggctgaa gctgttccgg gttttcaacc gctcgtctca gcctgcagca tgaagaaagg
      721 cccgccgaat ttcggcgggc cttttgtttt gcagtggtgg gggcctaagg gatgaccgcg
      781 gtgtagacgt tcgatggtgt gctttccacg cccgacccat ctacactcgt cacttcatag
      841 ttgtaagtca ctccgctttg caccgtggtg tcggtgtagc tggtcggtgt gttgaccgcg
      901 gtgttcagct tctggtaagt gcctgcgccg gtggagcggt agatgttata gctcaccacc
      961 ggatccgcac tgctcgcggg cgccgtccag ctcaattgga cctcgtaggt tgcagagttg
     1021 actccggtgc cggtcagcgc gatggtctgc gttgcgccgg tggtcgcgtt gctggtgatg
     1081 gtcaccgagc cggtggctgc gcctgccgtg gttggatcga actgcaggtt cagcaccgcg
     1141 gtctggcccg ggttcagcgt cagcggcaag gtcagccccg acatcgtgaa gccggttcca
     1201 gtaatagtgg ctgcactgat tgttaccgcg gatccgccgg acgaccgcag cgtcaccgtt
     1261 tgcgttgacg gcgtgttcag gttgacgttg ccgaatgcca cggtggtgga gctgagcgtc
     1321 aggccaggcg cgccgttggt gagctggatg gcgaaagtct tcacgacccc gcctgccgat
     1381 gccgtcagcg tggctgtttg ggttgaggtg accgcggtga cggctgcgtt gaaggacgcg
     1441 gttgtcgaac ctgccggcac tgtcaccgac gcggggaccg taaccgcgcc gttgctgctg
     1501 gccagtgtta cggcaaatcc gccggttccc gcagcagcgc tcagcgtaac ggagcaggca
     1561 tcggtgccgg ccgcgttata cgacgaggtg gcgcaggtca gcgaactgag cacgcccacc
     1621 ggggcgactc ctgttccggt caatgcaatg gccgggttgg gattggtcgc ggcattgctg
     1681 gtgatcgcga tcgatccggt tgcggctccc gccacggtcg gattgaaggt cacctgcaag
     1741 gtcgcagttt gtccggaatt cagcgtcacc gggaaggtgg ctccggtcat cgagaatcct
     1801 gttccggtga gggtcgcggc actgatcgtg agcggcgcgg tgcccgagga ggtcagggtt
     1861 accgacttgt tggttggcgt gttcaggttg acattgccga aggccagcgt ggtggagctc
     1921 acggtcaggc caggcgctcc gctcaactga atggcaaagg tctttgccac tccgcttgcg
     1981 gttgccgtca gggtggctgt ttgcgtggaa ctcacagcag cgacggtggc gttgaatgtg
     2041 gcggtggtcg cgccagccgg cacggtcacc gaagccggca ctgtgacaga gctgttgttg
     2101 ctggcgaggg tcaccgcgag gccgccggtt cccgccgccg caccgagtgt gacggtgcag
     2161 gcatcggttc cggccgcggt ataggacgac gtggcgcagg tcagcgaact cagtgcgccg
     2221 gccgcggcat caccggttcc gctcagagtg atgttggcgg tgggattcgt cgtggcgttg
     2281 gtcgtgatca ggatggatcc ggtggctgct cccggcgtcg tgggattgaa cgtcacctgc
     2341 agcgtgacgg tctgtccagg attgagggtc accggggggt tggctccggt catggagaag
     2401 ccggtccctg tgagagccgc ggtattgatc gtgacgggcg atgtgcccga cgaagtcaag
     2461 gtcaccggct gactggccgg ggtgccgacg gtcacgtctc cgaaagccag attcgtggtg
     2521 ctcacgctca gcaccatggt ttgtgcgttc agctgcagag tgaagacctt ggtgctgccg
     2581 ccggatgcgg cgctcagcgt cgcggacgtc gatgatgtca ccgcggaaac cgtggcgttg
     2641 aagctcgccg aggttgagcc tgcggggacc gtgaccgaac ctggcacggt cactgaggaa
     2701 ctgttgctgg aaagggtgac gacctgtcca ccgatcgggg ctgccgcact gagtgtgacc
     2761 gagcacgggt cagtcgcggc gccggtcagc gtctggttgg tgcaggaaag ggaactgagc
     2821 acaccggctg ccgactgtcc gttcccgctg aggctgatca caatcgttcc attcgaggca
     2881 ttgccggcga tcgtgatgga accgttggcc gcgcccgcgg tggtcggatc gaagctgacc
     2941 tgcagcgtgg cggtctgtcc gggattcagt gtgagcggga atgtcgcccc ggtcatcgag
     3001 aagccggcgc cggccagggt tccgctgttg atcgttaccg cagacgaacc ggtcgatgtc
     3061 agcgtgaccg gctgtgtggt ggtggtgttg aggttgacgt cgccaaagtt gagcgtcgtc
     3121 gtgctcgcgc tcagtgccgg gttctgcgcc agcaaccgca cggcgtagat cttcgaagcg
     3181 ccgccggcgc tcgcggtcac gttcgcggtc gcgtttgagg tcaccgctgt agcggtggcg
     3241 gtaaagaatg cattcgtatt gccggccgca acggtcacgc cggagggaac ggtgagggca
     3301 ctgttgctgc tggacaggct gacggccagc ccgcctgcgg gcgcagccgc agtgagcgag
     3361 acggtgcagt tgtcggtgcc cgctccggta atcgtcgagc tgtcgcagaa gaggccgttg
     3421 agaacgccgg cggccgcatc gccggttccg ttcagcgcta cgacggcggt gccgccggtg
     3481 ggcgcgtccg aggccacggt cagggatccg gttgcagcac cggcgctcgt gggactgaag
     3541 ttcacctgca gggtgaaggc atttcctgga ttcagcgtga tcggcaatgc gggaccggac
     3601 acagagaatc ctgcgccgct cgccgtcgcc gcgctgacgg tcaggggggc ggtgcccgtg
     3661 gagatcacgg tgacgaactg cggcgtcgcc tggtcgttca cggtgacgtc gccgaaggaa
     3721 agggtgctgg cgctcaggct catcgccggt gtacccgtgg gtaccggggc ggtgccgttt
     3781 ccgctcagcg ccactacggc ggtcccgccg gtgggcgcgt cggaggtcac ggtcagagag
     3841 ccggtcgctg tgccggtatt cgccggtgtg aagttcacct gaagggtaac ggtgctgcct
     3901 ggattcagcg tgatggggaa tccggggccg gccgtcgaga atcctgcgcc gctcgctgtt
     3961 gccgcgctga cggtgagcgg tgcggtgccg gtggagagga cagttacgaa ctgcggtgtc
     4021 gcctgggtgt tgacggtgat gctgccgaag gaaagagcgc tggcgctcag gctcatcgcc
     4081 ggagttccgc cactgctcgg cagggggttg agctgcaggt taaaggttgc ggaactggtt
     4141 cccatcgccg ccgtgatgat cgcggtctgc gctgcgctca ctgccgccgc ggtgacggta
     4201 aagctggcgc tgctcatgtt gggcgcaacc gcaaccgtgg cgggaaccac cgctgccgaa
     4261 tcgttgctgg aaagattgac ggtgccgcca ctgctgtcaa cggcggcgct cagggtcacg
     4321 gtgcaattgt ctgtgccggc gccggtgatc gtgctgctgc tgcaggcgat ggcgctgact
     4381 gtgggcggaa ccggagtcgg gggaggagga gtggtgactg ctgtcgttcc tgttccggtg
     4441 agagacacgg taacggtgcc ggatgcggca ttgctcacaa cggtcagctt tccgctcgcg
     4501 gctcctgccg ccgtgggatt gaactgaagg ttcagagtga gggagcctgc tgctgccagc
     4561 gtcatgggca ccgcggtctg gctggtcaga gcaaaggact gtccggctac gctgatttgc
     4621 gtaatctgca cggccgccac gctgtcgttc ttgagcgtga tcgtattgct ggcggtctgg
     4681 ccgaccgtaa cgttaccgaa cgcgagtgtg ccagtcgacg ctcccagggt gcctacggtt
     4741 gcggcatttg actcaatgct tccgcagccg gacagcgcaa gaggaagaac gcacagcgtc
     4801 atcagcagaa gaaccgcaca ccatgcgggg tacttttcaa aatgggtacg gcccgctgaa
     4861 cggtgacggg aaatcggcga attgcctgaa gacgcgcgga cacaaccgcg ggatctcccc
     4921 gcggggggaa gtgtatcgat cgagtgcatc tacctcacct cttgcaaaac aaaaaaagtt
     4981 caatactgcg gaccacgtcg gcctgaggcg ccggaatgtc ggagctcctc agtagccggg
     5041 cgggcccact tctttggtcc tgcttgctca gccccggttc aaggttgaac tttgcggata
     5101 acctgtgcct cgtgtaggca cgtgggtcac catccttttc aacagcaggt tacctttgtg
     5161 tttttggcgt gatatgtcgt atttatga
//
LOCUS       DASZYN010000824         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_881536, whole genome shotgun sequence.
ACCESSION   DASZYN010000824 DASZYN010000000
VERSION     DASZYN010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881536"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VHE33_20585"
     CDS             <1..460
                     /locus_tag="VHE33_20585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99909.1"
                     /translation="MLPPDGSNRGLDAALLPGGDTRRTGAVNEKFVTGCAKPPRQFWF
                     ALCSASFEVIQLATFVALEVMMMLFAGHFVARSIARQFNRLQPSLLNQRLDVSVHRGN
                     SNARVELLRRSESFFRRQRPIGFRKSLSNGSLLSGVTNVSDAQSNLLCAD"
     gene            565..1431
                     /gene="lgt"
                     /locus_tag="VHE33_20590"
     CDS             565..1431
                     /gene="lgt"
                     /locus_tag="VHE33_20590"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HVT99910.1"
                     /translation="MAPPLSSLISARYWIDRLNPWVVHFGNGFGVRWYGFAYLAGFLL
                     AGWILARWARRGKLPIPETEVASFILYAALGVMMGGRLGYCLLYNLHEVLRRPLEILE
                     IWHGGMASHGGMAGLLVAIWLVARRRGLNTLSLLDAAAATGPIGIALGRIANFVNGEL
                     WGRPTCVPWAVVFPDAPLVDGHQVPRHPSQLYAAGIEGMLVFLVGLAVYGKSSRPGLT
                     TAAVLTVYGAGRFIDEFWRQPDLGQPVYWGWMSKGQLLTIPMIVVGFSWLLWHRVHAV
                     DMGKKTSLVECD"
BASE COUNT          281 a          454 c          454 g          356 t
ORIGIN      
        1 aatgctccct cctgatggtt caaaccgagg gttggacgca gcgttattgc ctggcgggga
       61 tacacgccgc acaggtgccg taaacgaaaa attcgtgacc ggatgcgcga aaccccctcg
      121 gcaattttgg tttgctctgt gcagcgcatc cttcgaggtc atacagcttg ccacattcgt
      181 tgcactggaa gtgatgatga tgctttttgc cggccacttc gtagcgcgta gcatcgccag
      241 gcagttcaac cggctgcagc catccctcct caaccagcgt ctggatgttt cggtacaccg
      301 tggcaattcc aacgcccggg tggagttgct gcgccgatcg gagagcttct tcaggcgaca
      361 gcggccgatt ggcttccgca aaagcctctc gaatggcagt cttttgtcgg gtgttacgaa
      421 tgtctcggat gctcaatcga atctcctctg tgcagattaa agcctccgca ggaggcggaa
      481 cccggccgct cagctacccc gcgaaatcag tcataatcgg gcgcactggg cgtttcgcta
      541 tgataatgat ataccattat cattatggcg cctcccctca gctccctgat ttcagcacgc
      601 tactggattg accgcctcaa tccctgggtc gtccatttcg gtaacggctt tggagttcgc
      661 tggtacggtt tcgcctacct cgcaggattc ctgctcgccg gatggattct tgcacgctgg
      721 gctcgccgag ggaaactgcc gattcctgaa accgaagtcg ccagcttcat cctctacgct
      781 gcgctgggcg tgatgatggg cggccggctg ggctactgcc ttctgtacaa cctgcatgag
      841 gtcctgcgtc gcccccttga gattctggag atctggcatg gcggcatggc aagccacggt
      901 ggaatggccg ggctcctggt cgccatctgg ctcgtcgctc gtcgccgcgg tctgaatacg
      961 ctctcgctgc tggatgcagc agcggcaacc gggccgatcg gcattgccct gggtcgtatt
     1021 gcaaatttcg tgaatggaga actgtggggc agacccactt gtgttccctg ggcggtcgtt
     1081 ttcccggatg cgcctctggt cgacggccat caggtgccgc gtcatcccag ccagctctat
     1141 gccgccggga tcgaagggat gctggtcttc cttgtaggcc tcgctgtgta cggcaaatcc
     1201 tcccggcccg gactcaccac ggccgctgtg ctgacggtct acggtgcggg gcggttcatc
     1261 gacgaattct ggagacagcc ggatctgggc cagcccgttt actggggatg gatgagcaag
     1321 gggcagctcc tgacgatccc gatgattgtt gttgggttct cgtggctcct ctggcacaga
     1381 gtgcacgcgg ttgacatggg gaaaaaaacg agcctggtcg aatgcgatta aaacttatcg
     1441 tcctgcgcga cgacgacaac caggtcaggg atgaccttca gaagaatcat ggagtcacag
     1501 gatgaaccct ctgcttctca cctcgatggc ggccgtttca acctc
//
LOCUS       DASZYN010000825         5742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_755319, whole genome shotgun sequence.
ACCESSION   DASZYN010000825 DASZYN010000000
VERSION     DASZYN010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755319"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..461
                     /locus_tag="VHE33_20595"
     CDS             243..461
                     /locus_tag="VHE33_20595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99911.1"
                     /translation="MRDRRYRVGKQQDCQPTGRSRLSGVTLATPAKIANQASVATTAT
                     IAAAAIRQLSLMQGSPQLSGKPAQYAVL"
     gene            461..1486
                     /locus_tag="VHE33_20600"
     CDS             461..1486
                     /locus_tag="VHE33_20600"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVT99912.1"
                     /translation="MRIFRTLVFLLCPLAAMAAQLPQAPASADNLAKAHEDLQQNRPD
                     QAIALLEQMRAMQPVPKGVDRELGLAWYRTGKLAEARAAFQQAMTEDPQDIEAVQMEG
                     LTLYRLGQPAAAIPYLERVRQWTPDAGTDANYVLGLCYLNANRSDDARKAFAAQFGVP
                     PDSGAAWMLLAAMLQKASLPRQAAPAAQKALDLEPNLPSAHLLLGEVALLDSNIDGAL
                     QQFEAEQKVNPNSAVVYERMGDAWLRIGKLDLAQQSLTRALSLDTSSTGPFILMGRVL
                     LRRQDARTAIMYLQHAEKMDPGNYITHASLAQAYRMAGQDDEAKRENDLAAQSHLKNE
                     LKLQPLQ"
     gene            complement(1492..2745)
                     /locus_tag="VHE33_20605"
     CDS             complement(1492..2745)
                     /locus_tag="VHE33_20605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99913.1"
                     /translation="MAAVNAGPDLEDALTTDERWALVKRIASSPHLKSSPRLCEFLFY
                     ISKCAIRNAPEDATEQQIGIRVFGRSPGYNSSEDSIVRTHARLLRQKLASYFSEDGAA
                     EQTVLEIPKGHYFPVFHNRNTEAPPVLFTPSLTGEMDVQDLPHPAEDKEPSRRRAIVA
                     TVVLVLLAVGIAGLALWRPWRASVAAPTAVDVFWHPFLSGDPPLVIYSNALFQGTSTQ
                     GLRYAPPDPTGPPFGDDYVDTYTGIGELNSVYTLTQLFDAHHATFLLKRSLLVTWDVA
                     QSRNLVFVGSRAENPSLRVLPSTTDFTMMATPDSAGIVNHHPLPGEPPVYLRPERPLN
                     TDYAILALQPGVQPGRLMLVFSGLTTFGTQAAVDFVCDPDSVAGLLKKISGPKGEIRP
                     FEAVLQTTVGGGVPFATRLVTIRIH"
     gene            2959..>5742
                     /locus_tag="VHE33_20610"
     CDS             2959..>5742
                     /locus_tag="VHE33_20610"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HVT99914.1"
                     /translation="MTSTRSKEFQGWAHQRRIPLALFAALLLMLVGTLPAVGQAVYGS
                     IFGTVTDKTGAVVPNATITVTDIAKATTTTVQTNEAGDYRVQHLIPDTYRVEVAAKGF
                     SNAQVDNVVVYADTAPEVNLQVDVGSVGTTVTVTGGAPLLQTDRADVSTILNERAVEN
                     LPNLNRNFTAFELLTPGTTYIGWGPGEGGGNPQRSQSIEVDGQLPFATGYELDGTDNQ
                     EPLNGVAVINPNLDAVSEMKVTAQNYDAEFGKAVAGLVTAQTKSGSNEFHGTAFEYRR
                     SDAQQARDPFANAPPNDKLAPTLHNQFGGSIGGPVLHDKLFFFADYQGLREKTGVSTL
                     TTVPTLKAETTCTSGGACDLSDYLSVGQIYKPNSLTDASNNGRTAYAGNVIPAGDLSA
                     AAVAFFKLLPKPNVSTTAVENNYSASGAGVFSTNQPDVRIDSKLNDKLHVFGRYTYFG
                     GSLSGAPFFGAAGGQGFGSGGFAGTDAFHYSSLASGGDYIVSNTWLTDFRFGYYRIYN
                     NTQGPNGNQALGNSLGIPNANVGDLSLNGGLPQFNIDIPANGANGGQNIEFGTSANPT
                     LQQTSQYQFVNNWSHSFGNHNIKFGADYRYGKNWTVTIASNALRSGTYFFASSRTSGV
                     SGTGDESPGVGFATFLLGDTTTFWRTQTANVKAQSRQTRFFTYAQDQWRATHNLTVNY
                     GLRWELYTPESLTASGAGGLLNLQTGILGIAGVGQFDNKANVTNNFGMFAPRLGVAWQ
                     ARPNTVVRAGYGIVYGQGWAGDSFGSVMTSSFPTQVQQTLNPVTNAAAVFNLTSTENG
                     VAPGPPGYTFAPIPTSGAYVLPDGISQIARPTQVRLPSVAGWNFTVEQQLSKDMSLQV
                     AYVGSQAWHNMFDSSPSYNANEITYKGFHQVNPNNTTPGGGSCAGLTYPTAPDSCLYT
                     NPQRMPYYDGTAQS"
BASE COUNT         1221 a         1821 c         1651 g         1049 t
ORIGIN      
        1 gtggtacttc ggcggctcct gctcctgggc ggcgagcgac tcttccctca ctcggccggc
       61 gtttgcgaac tccggaagtc ctgccatcat cgccatcgat cccatcgctc cggcgcgctt
      121 gagaaaatcg cgtcggtctg ttcctttgct gtccatcatc cctccgcccc tgttttgttt
      181 gcgatggcaa caataacacc gtggagacgg tttgtgcagg gaggaagttt ctgaggttca
      241 gaatgaggga ccgcaggtac cgcgtcggaa agcagcagga ttgccagccg accggaagat
      301 cgcgcttgtc cggagtcacg ctggcgacac cagccaaaat agcgaatcag gccagcgtgg
      361 caacgactgc gacgattgca gcggccgcca tccgacaact cagcctcatg caaggctcac
      421 cccagctcag cggaaaacca gcgcagtatg ctgttctctg atgcgtattt tcagaaccct
      481 cgtttttctg ctctgccctc tcgcggccat ggcagcacag cttccccagg cccccgcttc
      541 cgcggacaat ctggcaaagg cccatgaaga tctccagcag aatcggccgg atcaggcgat
      601 cgcgctgctc gagcagatga gagcaatgca gcccgtccca aagggcgtgg atcgcgaact
      661 gggcctcgct tggtatcgca ccggcaagct cgccgaggcg cgcgcagctt ttcagcaggc
      721 gatgaccgaa gatccacagg acatcgaggc cgtgcagatg gagggactca cgctctatcg
      781 actcggtcag cccgccgcgg ccattccgta tctggaacgg gtgcgccagt ggacgccgga
      841 cgcgggcacg gacgccaatt acgttttagg tttgtgttat ctgaatgcca atcggagcga
      901 tgacgcgcgg aaggcgtttg ctgcgcaatt cggggtacca ccggactccg gcgcggcatg
      961 gatgctgctg gcggccatgc tgcagaaggc gagcctgccc cgccaggccg cgccggcagc
     1021 gcaaaaggcg ctcgacctcg aacccaatct cccgtcagcc caccttttat tgggagaagt
     1081 ggcgcttctc gattcgaata tcgacggagc tctgcagcaa ttcgaggccg agcagaaggt
     1141 caacccaaac agcgcggtgg tatacgagcg gatgggcgat gcctggttgc gcatcggcaa
     1201 actcgatctg gcgcagcagt ccctcacccg tgcgctctct ctggatacca gcagcaccgg
     1261 cccattcatc ctgatgggcc gggttctgct tcggcgacag gatgctcgca ccgccatcat
     1321 gtacctgcag catgccgaga agatggatcc gggcaactac atcacccacg cctctctcgc
     1381 ccaggcgtat cgcatggctg ggcaggacga cgaggcgaag cgcgaaaacg acctcgcagc
     1441 tcaaagccac ctgaaaaacg aattgaaact gcagccgctg cagtaacggt ttcagtggat
     1501 gcgaatcgtg accaggcgag tcgcgaacgg aacgccgccg ccgaccgtcg tctgcaacac
     1561 ggcttcgaac ggacggatct cacctttcgg accggagatc ttcttcagca ggcccgcgac
     1621 actatccgga tcgcacacaa aatccaccgc agcctgggtg ccgaatgtcg tgagcccgct
     1681 gaagaccagc atgagccggc ccggttgcac tcccggctgc agagccagaa tcgcgtaatc
     1741 ggtgttgagt ggacgctccg gccgcaaata caccggcggc tctccgggaa gcgggtggtg
     1801 attgacgatg ccggccgagt caggggtggc catcatcgta aagtcggtcg ttgacggcag
     1861 gacccggaga gacggattct ccgccctcga tccaacgaag accaggttac gtgactgtgc
     1921 cacgtcccac gtaaccagca aactgcgttt cagcaggaag gtggcatggt gcgcgtcgaa
     1981 aagttgggtg agcgtgtaaa cggagttcag ctctccgatg cccgtatagg tgtcgacgta
     2041 atcgtcgcca aacggcggcc cggtcggatc cggaggggca taacgcagac cctgggtgga
     2101 ggttccctga aagagcgcat tgctgtagat gactaagggc gggtcgccgg aaaggaaggg
     2161 atgccaaaac acatccactg ccgttggagc cgccacgctg gctcgccatg gacgccacag
     2221 ggccagaccg gcaatgccaa ccgccagcag cacgagtacc acggtcgcga cgatcgcgcg
     2281 ccgccgcgaa ggctctttat cttctgcagg atgcggcaga tcctgcacgt ccatttcacc
     2341 cgttaaagac ggggtgaaaa gcaccggcgg ggcttccgta tttcgattat ggaaaacggg
     2401 aaagtaatgg cccttgggga tctcaagaac agtctgttcc gcggcgccat cctcagaaaa
     2461 gtacgacgcc agcttctgcc ggagcagacg cgcatgggtc cttacaatgc tgtcttcgct
     2521 ggagttgtat ccaggagaac ggccgaagac gcggataccg atctgttgtt cggtggcgtc
     2581 ttcaggagca ttacggatag cgcacttcga tatgtaaaac aggaactcgc agagacgcgg
     2641 ggatgatttc aggtgcgggc tcgaagcgat ccttttaact aaagcccatc gttcatccgt
     2701 cgttagcgcg tcttcaagat ccggccctgc attaactgca gccattgcaa tagttcgcag
     2761 tatggcatac gcaaatttgc cggtcaattc accgtaaaaa acgttgcggg tccatctcgc
     2821 tgacaagaat atcgcggttg ttgattcaag tgtccgatcc tctgcacgcc ctcgttcgcg
     2881 cagagaggcg actccccctt gcgggagcct taaaccaaaa acaatgcgga ccccaacgtt
     2941 tttccggagg caatgcgtat gacgtcgaca agaagtaagg aattccaggg atgggcacac
     3001 cagcgcagga ttcccctggc actcttcgca gccctgctgc tgatgctggt tggaaccctc
     3061 ccggctgtcg gccaggctgt ttacggatcg attttcggca ccgtcacgga caagactggc
     3121 gccgtagttc cgaacgccac gatcacggtc accgacattg ccaaggcaac gacgacaacg
     3181 gtgcagacca acgaagccgg tgactatcgc gtgcagcacc tcatccccga cacttatcgc
     3241 gtggaagtgg cggcgaaggg cttctcgaat gcgcaggtcg ataacgtcgt ggtctacgcc
     3301 gacacagcgc cggaagtgaa cctgcaggtg gatgtcggct cggtaggaac caccgtgacc
     3361 gtcacgggcg gcgctcccct gctgcaaacg gaccgcgccg atgtgtcgac catcctgaat
     3421 gagcgcgcgg tcgagaacct gcccaacctc aaccggaact tcacagcgtt tgagcttctc
     3481 accccgggca cgacctacat cggatgggga ccgggcgaag gcggcggcaa tccgcagcgc
     3541 agccagtcga tcgaagtcga cggacaactt ccttttgcca ccgggtacga gttagacggc
     3601 accgacaacc aggagccgtt gaacggcgtg gcggtcatca atccgaatct tgacgcggtt
     3661 tccgagatga aggtcaccgc gcagaactac gacgccgagt tcggcaaggc agtggcgggt
     3721 ctcgtgaccg cgcagaccaa atccggcagc aacgagtttc acggcacggc gtttgaatat
     3781 cggcgctcgg atgcacagca ggcgcgcgac cccttcgcga atgcgccgcc caacgacaaa
     3841 ctggcaccca ccctccacaa tcagtttggc ggctccatcg gcggtcccgt tttgcacgac
     3901 aagctgttct tcttcgcgga ctatcagggg ctgcgcgaaa aaaccggcgt gtccacgctg
     3961 accacggtcc ccaccctgaa ggcggaaacc acttgcacct cgggtggcgc ctgcgatctg
     4021 agcgactatc tctcggtcgg acaaatctat aagccaaatt cgctgaccga tgccagcaac
     4081 aacggccgta cagcgtatgc cggcaacgtg attcccgccg gcgatctctc tgctgcggcg
     4141 gttgctttct tcaagctgct gcccaagccg aacgtctcaa ccaccgcggt tgaaaataac
     4201 tactccgcct caggcgcggg tgtattcagc acgaaccagc ccgatgtgcg tatcgacagc
     4261 aagctgaacg acaagctgca cgtcttcgga cgctatacat atttcggcgg cagcctgtca
     4321 ggcgcgccgt tctttggggc agccggcggt cagggcttcg gctccggcgg atttgccgga
     4381 accgacgcgt tccactattc gagcctggct tcgggcggcg actacattgt ctccaacacc
     4441 tggctcaccg acttccgctt cggctactac cgcatctaca acaacaccca gggtccgaat
     4501 ggaaaccagg cgctgggcaa cagccttggc attcccaacg ccaacgtggg ggatctgtcg
     4561 ctgaacggtg gcctcccgca gttcaatatc gatatccccg ccaacggcgc caacggcggc
     4621 cagaacatcg aattcggaac gtcggcgaat cctactctgc aacagaccag ccagtatcag
     4681 tttgtgaata actggtcgca ctccttcggc aaccacaaca ttaagttcgg agccgactac
     4741 cgctacggca agaactggac ggtcaccatc gccagcaatg ctctgcgttc cggcacctac
     4801 ttcttcgcat cctcgcgcac ctccggggtc agcggtacgg gcgacgagtc gccgggcgtc
     4861 ggcttcgcca ccttcctgct cggagatacc acgaccttct ggcgtacgca gaccgcgaac
     4921 gtcaaggccc agtcccgcca gacgcgcttc tttacctacg cgcaggatca atggcgcgcc
     4981 acccataacc tcaccgtcaa ctacggcctg cgctgggaac tctacactcc cgaatccctc
     5041 accgcatcgg gagccggtgg ccttctgaat ctgcaaaccg gcatcctcgg tatcgcgggg
     5101 gtaggtcagt tcgataacaa ggcgaacgtc acaaacaact tcggcatgtt cgcgccacgc
     5161 cttggcgtag cctggcaggc gcggccgaac accgtcgtcc gcgcgggcta cggaattgtc
     5221 tatggacagg gctgggccgg cgacagcttc ggcagtgtca tgacctcatc cttcccaacc
     5281 caggtgcagc aaaccctcaa ccccgtcacc aatgcggccg cggtatttaa cctaaccagt
     5341 actgaaaacg gggttgcgcc tggacctccc ggctacacct tcgcgccgat tccgaccagc
     5401 ggtgcatacg ttctgcccga tggaatttct cagatcgcgc gcccgacgca ggtccgcctg
     5461 ccctccgtcg ccggctggaa cttcaccgtc gagcagcagc tcagcaagga catgtcgctg
     5521 caggtcgcct atgtcggcag tcaggcgtgg cacaacatgt tcgactcttc gccctcttac
     5581 aatgcgaacg aaatcaccta taagggattc caccaggtca accccaacaa cacaacaccc
     5641 ggtggcggga gttgcgcggg tttgacttat ccgacggcac cagacagctg cctctatacc
     5701 aatccgcaac gcatgccgta ttacgacggc acggcccaga gc
//
LOCUS       DASZYN010000826         6624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1215648, whole genome shotgun sequence.
ACCESSION   DASZYN010000826 DASZYN010000000
VERSION     DASZYN010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215648"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VHE33_20615"
     CDS             <1..549
                     /locus_tag="VHE33_20615"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVT99915.1"
                     /translation="PESGVPFGEILTAGKLAALENEARTAPDPAKAHEFSTHSFGAQF
                     IEVAWDPGIARLRVSRALSVIDAGRIINHKAGRNQILGAVVMGIGMGMFEETIYDPRN
                     AKPINNNFADYIVATNADIPELDCIFVEYPDLHLNEYGARGIGEIGLAGIASALTMAV
                     YHATGVRVRKLPVRIEDLLASS"
     gene            750..1793
                     /locus_tag="VHE33_20620"
     CDS             750..1793
                     /locus_tag="VHE33_20620"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HVT99916.1"
                     /translation="MSELRRILDLWKRASAANDEICLATVVAVEGSAYRRPGARMLLT
                     SSGQRAGTVSGGCLEAEIAKKAWWLTEQGPSLQRYSSFFDDDGDMPYGLGCGGTVIVL
                     LERGEAAAQHLEALRRSVEDRTDSVIVTCIDPAAPGTLLILNEADCAVYDRHLDEEAT
                     RLAHDALRTGKSSRAGAYFIEHIAPPPALILFGAGDDVQPLVEFASALGWHVSVADDR
                     SNLARPERFPNAVVRNLDGALAQITPTAAAVIMSHSYEQDRKVLRALLARDMKYIGVL
                     GPRPRTERLVAEIAPLLELTPTDCMARLHTPVGLDLGGHSPASIALSIAAELQAVFAG
                     REARKPSSPSVHA"
     gene            1786..2391
                     /locus_tag="VHE33_20625"
     CDS             1786..2391
                     /locus_tag="VHE33_20625"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HVT99917.1"
                     /translation="MPEFRCAAVVLAAGASTRLGQPKQLIRIGSQSLLRRTVRLAIEA
                     GCSPVYVVLGFESDQMTPELTGLAVETVINPAWQQGMGSSLRAGMQALLRAPTQPDAV
                     LLLVCDQPRLTAEHLRELLTWHWAARTKSEIAITASTYAQRNGVPAVFSTKLLPELLA
                     ITGDHGARDLIRAHARQVQGIPWAAGEFDIDRPEDLNTIEA"
     gene            2651..3442
                     /locus_tag="VHE33_20630"
     CDS             2651..3442
                     /locus_tag="VHE33_20630"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HVT99918.1"
                     /translation="MAWYAYCITERQAFPELLRHRKPIPMEAVTGIQGNQIFLYPASD
                     LAVIVSEHSPSQNLSSQAGVEHARVISDCFKTSTVLPFRFGTVFADDDALRRSIRSNQ
                     RQFLTNIERLRGKAEMHLKVLLDDCCREQVRRYSAEPIPTVGRAWLSNLRENAALQRE
                     RQTKARAVTVQMHRMFLPLAEEVSCKRMESGKMLLDIAHLIDSNGIERYQNKYSSASV
                     LMKDCHMQLSGPWPPYHFVHRLTHHNSPAVAGNSVRPSAHLAASA"
     gene            3672..4625
                     /gene="fabD"
                     /locus_tag="VHE33_20635"
     CDS             3672..4625
                     /gene="fabD"
                     /locus_tag="VHE33_20635"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HVT99919.1"
                     /translation="MTNLAFLFPGQNSQYAGMGRDLAEKFPVARRTFEEADEALGFAL
                     SKLCFEGPEDQLKLTEFQQPAICTVSVAALRVLAEKGITPNWVAGHSLGEYAANVAAG
                     SLAFADAVRTVRSRGRFMQEAVPAGQGAMAAVLGLSSEETARACDDAAAETKQVVSAA
                     NFNSPEQTVISGEAPAVERAAEILKERGAKRVVMLQVSAPFHCALMQPAQDRLAELLH
                     AVTFAQPAIRVAVNVNATLVGDPAELRDALIRQVTGVVRWVDSVRLLISLRPSFFVEV
                     GPGKVLTGLMRQIDRSQSCVNIEDEASLERVIQGAKQKAQG"
     gene            complement(4700..5179)
                     /locus_tag="VHE33_20640"
     CDS             complement(4700..5179)
                     /locus_tag="VHE33_20640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99920.1"
                     /translation="MTLMDAPAFNARKAQQTRVLAITGVVVLAVAIIGTILWFLQVPW
                     QFWHWPSDHKVDQFMAAVESGDMNKAYGLWNNDANWQQHPQQYQPYGMTEFTKDWGPS
                     SDYGTITSHQIFVSHRAGNGVIIGLYINGNKGKPLFLRVDSATKTIGFSPVELYSGP"
     gene            complement(5386..6567)
                     /locus_tag="VHE33_20645"
     CDS             complement(5386..6567)
                     /locus_tag="VHE33_20645"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HVT99921.1"
                     /translation="MKKIRVGILFGGRSGEHEVSLLSAASVLGAIDRKKYEVVPIGIT
                     KEGRWVTAGHAERLLGGDESGKHLRAGDPASTQPAAVLRKGESVLVPPVPSEALIPFE
                     RDAGAVTHAGPEQMLSVDVIFPVLHGTFGEDGTIQGLFELAGMAYVGSGVLGSAAGMD
                     KDAMKRLFASAKLPMVKWVTFLRGEWEKSPKKVRAKIEATLKYPVFVKPANLGSSVGI
                     TKAHSAKELGPAIETAAQFDRKIVVEQGVGGKNGKARELEVAVLGNDAPEASVVGEVV
                     PIKEFYDYEAKYLSEGSDLIVPAKLNRAQTKQVREMAVAAFRACDCSGLARVDFLMEP
                     GKTGRIYVNEINTLPGFTSISMYPRLWAASGVAYPKLIDRLIELGMERRADKDRNKYS
                     L"
BASE COUNT         1248 a         2374 c         1700 g         1302 t
ORIGIN      
        1 cccgaatccg gcgtcccttt cggcgaaatc ctcactgcgg gcaaactggc cgctcttgaa
       61 aacgaagcgc gcaccgcccc cgatccggcc aaggcgcacg aattctccac ccactctttc
      121 ggcgctcagt tcatcgaagt cgcgtgggat ccgggcatcg cccgtcttcg cgtcagccgc
      181 gctctcagcg tcattgacgc cggccgcatc atcaaccaca aagccggccg caatcagatc
      241 ctcggcgccg tcgtcatggg catcggcatg ggcatgttcg aagaaaccat ctacgacccg
      301 cgcaatgcca agcccatcaa caacaacttc gccgactaca tcgtcgccac caacgccgat
      361 attcccgaac tcgactgcat tttcgtggag tatcctgatc tccacctgaa cgaatacggc
      421 gcgcgcggca tcggcgaaat cggtctggcc ggcattgctt ccgctctcac gatggctgtg
      481 tatcacgcga ccggcgttcg ggtgcgcaag ctgcccgtcc gcatcgagga tctgctcgcc
      541 agcagctaac ggcatgatcg accctccatt gggtgcccca catccctggt ttggggataa
      601 ggtgccgcgt gagcggcact acggtagaag ccccggcctt caggcccgga gcttttgtat
      661 cagggcacga gttcactcgt gccgataggt gccaaaataa agacgggctt tagcccctgt
      721 gctctttgat ccggcaagtt ggcagcaaaa tgtccgagct ccgtcgcatt ctcgacctgt
      781 ggaagcgcgc cagcgctgca aacgacgaga tttgcctcgc caccgtcgtc gcagttgaag
      841 gctccgccta ccgccgcccc ggggctcgca tgctgctcac gtcctccggt cagcgtgccg
      901 gaaccgtcag cggcggctgc cttgaagccg aaatcgcaaa gaaagcctgg tggctcaccg
      961 aacagggccc ctccctccag cgctactcca gcttctttga tgacgacggc gacatgccct
     1021 acggtctcgg ctgcgggggc accgtcatcg ttctcctcga acgaggcgaa gcggccgcac
     1081 agcacctcga agccctgcgt cgcagcgttg aggaccgcac cgactccgtc atcgtgacct
     1141 gcatcgatcc ggcagcgccc ggcacactgc tgatcctgaa tgaagcggat tgcgctgtct
     1201 acgatcgcca cctagacgag gaggccaccc gcctcgccca cgatgccctg cgaacgggca
     1261 agtcctcacg cgccggcgcg tactttatcg aacacatcgc accgccaccc gccctcatcc
     1321 ttttcggcgc cggagacgat gtccagcccc tcgttgaatt tgcctccgca ctcggctggc
     1381 atgtcagtgt cgccgatgac cgatccaatc tcgcccggcc cgagcgcttc cccaatgccg
     1441 tcgtgcgcaa cctcgacggc gccctcgccc aaattacccc cacggccgcg gctgtcatca
     1501 tgagtcacag ctacgagcag gaccgcaaag tgctccgcgc gctgctcgcc agggacatga
     1561 agtacatcgg cgtcctcggc ccacggcccc gcaccgagcg cctcgtcgct gagattgccc
     1621 cgctgctcga actcaccccc accgattgca tggctcgcct gcatactccg gtcggcctcg
     1681 acctcggcgg ccactcgccg gcttccatcg cactctccat cgctgccgaa cttcaggccg
     1741 tctttgcagg ccgtgaagcc cgcaagccgt cctcgccctc ggttcatgcc tgaattccgt
     1801 tgcgcggccg ttgtcctcgc cgcgggcgcc tccacccgcc tcggtcaacc caaacaactt
     1861 atccgtatcg gcagccaatc tctcctccgc cgaaccgtcc gcctcgcgat cgaagcagga
     1921 tgctcgcctg tatatgttgt gctcggattt gaatccgatc agatgacccc tgagctgacg
     1981 ggcctcgccg ttgaaaccgt tatcaatcct gcctggcagc agggaatggg ttcctcgctg
     2041 cgagccggta tgcaggcgtt gctccgggca cccacacagc ccgatgccgt gctcctgctc
     2101 gtctgcgatc agccacgcct caccgctgaa caccttcgcg aactcctgac ctggcattgg
     2161 gcggcccgga ccaagagcga aatcgccatt accgcatcca cgtatgcgca aagaaacggc
     2221 gtccccgctg tcttttccac aaagctgttg ccggaattgt tagcgattac aggcgaccac
     2281 ggcgcgcgcg atctgatccg ggctcatgcc cgccaggtcc agggaattcc ctgggccgcc
     2341 ggcgaattcg acatcgaccg gccggaagac ctcaacacaa ttgaggcgta attctagtcc
     2401 tctgaatggc aaaagtgatc acttggatgc tggtatttta cattgcgcaa ttattgttca
     2461 taacgtgaaa accagtttcg attctgatgc taacctcccg catcgaggcc ccgccaagtg
     2521 ttgccaggcg tttctctgcg gggctagcat acgttgcagt tcagtcaaga ctgcgatcga
     2581 aagccatgtc catccccaca acgctgaagt tcccaaccct ttcccaatcc agcatgcgga
     2641 gcgatccaat atggcgtggt atgcctactg cataacagaa cgtcaagcct ttcccgaact
     2701 gcttcgtcac cgtaagccga ttccgatgga agcagttacg ggaatccagg gcaatcaaat
     2761 tttcctttac cctgccagtg atttagccgt tatcgtcagc gagcactctc cctcacaaaa
     2821 cttgtcctct caggccggag tcgagcacgc ccgagtgatc tctgactgct tcaaaacctc
     2881 caccgttctt cccttccgtt tcggaaccgt ctttgcggac gatgacgcgc tccgccgctc
     2941 catccgctcc aatcagaggc aattcctcac caatattgaa cgcctccgcg gcaaggccga
     3001 gatgcacctg aaggttctcc tcgacgattg ctgccgcgaa caggtccgcc gctattccgc
     3061 cgagcccatt ccaacggtcg gcagagcctg gctttccaat cttcgcgaga acgccgctct
     3121 ccagcgcgag cgtcagacca aggctcgcgc cgtcaccgtc cagatgcatc gcatgtttct
     3181 cccgctggcc gaagaagtca gctgcaagag aatggaatca ggcaagatgt tgctggacat
     3241 tgcacatctg atcgactcca acggcatcga gcggtatcaa aacaagtatt ccagcgccag
     3301 cgtgctcatg aaggactgcc atatgcagct ctccggtccc tggccgccgt accacttcgt
     3361 tcaccggctc acgcatcaca actcaccggc ggtcgccggc aacagcgtac ggccttcggc
     3421 ccaccttgct gcctccgcct gacccacggt cctgcaaaaa agaaaagccc cggcggaccc
     3481 acgaaccacc ggggctttca tggagggcag caaggggctc ggcatttctg ccgggccccg
     3541 cgcgcctctc gtcttataga cgcgcgagac ccgcagaaag ttaatttgaa gttttccagc
     3601 tgggcgacgt tattcggtgt gccggatcac cggcacgggt cgcgcgcccc tgctaatctg
     3661 cccagagaga gatgaccaac ctcgcctttc tttttccagg acagaattcg cagtacgcgg
     3721 gtatgggccg cgatctggcg gagaaatttc ccgttgcccg gcgcaccttc gaagaagccg
     3781 acgaggctct gggattcgcc ctttcgaagc tctgcttcga aggtcccgaa gaccagctca
     3841 aactcactga attccagcag cctgccatct gcaccgtctc cgtcgcggcc ctgcgggtcc
     3901 tggcggaaaa gggaatcact cccaactggg tcgccggcca ctcgctcggt gagtatgcag
     3961 ccaacgttgc cgccggatcg ctcgcctttg ccgatgccgt gcgcaccgtg cgcagccgcg
     4021 gccgtttcat gcaggaagcc gtccccgccg gtcagggcgc gatggccgcc gtcctcggtc
     4081 tctcgtcaga agaaaccgca cgcgcctgcg acgatgctgc tgccgaaacg aagcaggtcg
     4141 tttccgctgc caactttaac tccccggaac aaacggttat ctcgggcgaa gcgccggccg
     4201 ttgaacgtgc tgctgagatc ctgaaagagc gcggcgcgaa gcgcgtcgtg atgcttcagg
     4261 tcagtgcgcc cttccattgc gcgctcatgc agccggccca ggaccgcctg gccgagttgt
     4321 tgcacgcagt cacattcgcc cagcccgcta tccgcgttgc cgtcaatgtg aatgcgaccc
     4381 tggtcggcga tcccgccgaa ctccgcgatg ctcttattcg ccaggtcact ggcgtcgttc
     4441 gctgggtcga ctccgtgcgc ctgctcattt cgctgcgtcc gtccttcttc gtcgaagtcg
     4501 gacccggcaa agtcctgacc ggcctcatgc gccagatcga ccgcagccaa agctgcgtca
     4561 atatcgaaga cgaggccagc ctcgagcgcg tgatccaggg cgctaagcaa aaagcccagg
     4621 gctaaagcaa aacatccctg gcgtatcgac gccagggatg tcggttccca aactacgccc
     4681 tattggctac tccctgcctc tacggccccg agtacaactc cactggagag aatccaatcg
     4741 tcttcgtcgc gctgtccaca cgcaggaaga gcggcttgcc cttgttcccg ttaatgtaga
     4801 gcccgatgat caccccgttc cccgcccgat gcgacacgaa gatctggtga ctcgtgattg
     4861 tgccgtaatc gctcgatggc ccccagtctt tcgtgaactc cgtcatcccg tacggctggt
     4921 actgctgcgg atgctgctgc cagttcgcat cgttgttcca cagcccatac gccttgttca
     4981 tgtcgccgct ctcaacagcg gccatgaact ggtcgacctt gtgatccgac ggccagtgcc
     5041 agaactgcca gggcacctgc aaaaaccaca gaatcgttcc gataatcgcg accgccagca
     5101 ccaccacccc ggtgatcgca agcacgcgcg tctgctgagc cttgcgcgcg ttaaaagcag
     5161 gtgcatccat cagggtcatc gagcatgact cctttcatcc gtccgaccca aaccagacat
     5221 tcgcacgtct cctcttattc tgacaccccg gatgcatcca tggttcccgg cagaaaacag
     5281 agagccctca gtcgaccttc gccccattga acgctcccag gctcgcctct cgttcgcctc
     5341 atcgctcgtc tctcactcgc ttgggttgct cgcagttcgc tgccctcaca ggctgtactt
     5401 attcctgtcc ttgtccgccc gccgctccat ccccagctcg atcagccggt cgatcagctt
     5461 cggatacgcc acgcctgacg ccgcccacaa ccgcggatac atactaatcg acgtgaatcc
     5521 cggcagcgtg ttgatctcgt tcacatagat gcgtccagtt ttccccggct ccatcagaaa
     5581 atccactctt gccagcccgc tgcagtcgca tgcccggaac gccgcaaccg ccatctcccg
     5641 aacctgcttc gtctgcgccc tgttaagctt cgccggcacg atcaggtccg acccctcgct
     5701 cagatatttc gcctcgtagt cgtaaaactc cttgatgggc acgacctcgc ccaccaccga
     5761 cgcctccggc gcatcgttgc caagcaccgc cacctccagc tcgcgcgcct tcccgttctt
     5821 tccgcctaca ccctgctcca ccacaatctt ccgatcgaac tgcgccgcgg tctcaatcgc
     5881 aggtccaagc tccttcgcgc tatgtgcttt cgtgatcccc accgacgacc cgaggttcgc
     5941 cggcttcaca aacaccggat acttcagggt cgcctcgatc tttgcccgca ccttcttcgg
     6001 gctcttctcc cattcgccgc gcagaaacgt cacccacttc accatgggca gcttcgccga
     6061 agcgaacagc cgcttcatcg cgtccttatc catcccggcc gccgaaccca gcacgcccga
     6121 tcccacatac gccatgcccg ccagttcgaa cagtccctgg atcgttccat cttccccaaa
     6181 agtcccgtgc agaaccggaa agatcacatc gacactcagc atctgctccg gccccgcatg
     6241 ggtcactgca cccgcgtcgc gctcaaacgg gatcagcgcc tcgctcggca ccggaggcac
     6301 cagcaccgat tcgcccttgc gcagcaccgc cgccggctgc gtcgacgcag gatcgcccgc
     6361 acgcaggtgt ttgccggact cgtcgcctcc cagcagccgc tccgcatgcc ccgctgtcac
     6421 ccagcggccc tctttggtga tgccgatcgg caccacctcg tactttttcc gatcgattgc
     6481 ccccaggacc gatgccgccg acagcagcga aacctcgtgc tcgccgctgc gcccgccaaa
     6541 aagaatgccg actcgaatct ttttcatgcg ccggctgtca ttctaaataa gacgcggcct
     6601 tcgccgctgt cgcgccggca actc
//
LOCUS       DASZYN010000827         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_755670, whole genome shotgun sequence.
ACCESSION   DASZYN010000827 DASZYN010000000
VERSION     DASZYN010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755670"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VHE33_20650"
     CDS             complement(<1..350)
                     /locus_tag="VHE33_20650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99922.1"
                     /translation="MKKSQFVFLLSLLLAVPAFAQESRQDISASGSMLNAPFASGNGV
                     QLHSTPGYGALISYRFQLTPHSALELNYQYAQNVQRFCCSFGSYHIHDRFQEFSAGYV
                     RSFYFRNWNPFIEAG"
     gene            complement(506..1456)
                     /locus_tag="VHE33_20655"
     CDS             complement(506..1456)
                     /locus_tag="VHE33_20655"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HVT99923.1"
                     /translation="MDLYQLETFLAVVEEKGFSRAAARMRRTQPAISHAIRKLEEEVG
                     EPLFERSSRDGTLTASGKLLRSYAERLLRLRGETITALEELRSLERGRLTIAANEYTC
                     LWLLPVLDAFRASYPHIGVTVQRSLASQIPDQLLERSAEFGILSFRPDDARLHAIAVY
                     TDSVTFVVDPKHPLARRKQVSLRELGKENFIAHTVASPLRHRVVETFARHQTPLNMGV
                     ELPSLEAIKRFVAYGNGVALVPGLTVQPELARGELVQVAVPELKIARRLWLVHRERVM
                     LSHAGAAFLEAVKTAVARHGHPFAFRAESPPLRGRRASKH"
     gene            1503..>1690
                     /locus_tag="VHE33_20660"
     CDS             1503..>1690
                     /locus_tag="VHE33_20660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99924.1"
                     /translation="MQMQRITNLLYGWAFAKLITYGAAWTLEPRAADRKATQQTRHCM
                     PQNQIYFFDTTLRDGEQSP"
BASE COUNT          360 a          476 c          559 g          295 t
ORIGIN      
        1 ccagcctcga taaagggatt ccagttgcgg aagtaaaagc tgcgcacgta gccggcggag
       61 aactcctgga accggtcgtg aatgtggtac gaaccgaagg aacagcaaaa gcgctgaacg
      121 ttctgggcat actggtaatt cagttccaga gcgctgtgcg gcgtcagctg gaagcggtaa
      181 ctgatcagag cgccataacc cggcgtggag tgaagctgga cgccgtttcc ggaagcaaaa
      241 ggcgcattca gcatggagcc gctggcgctg atgtcctgac ggctttcctg cgcgaaggcc
      301 ggcaccgcca gcaggaggga caacaaaaac acaaactgcg atttcttcat tcgtcggtca
      361 acctcttcac aacaccttcg ggagtttttc ggcgtacgag cgcgatggaa gcctcagagg
      421 ctccctgaaa tagcaaacac tgtgggaatg tccacccggt cattgtacct gccgaggcga
      481 agtttccaca gtcctgccga ggacgttaat gcttggacgc acgacgcccc cggaggggag
      541 gcgattcggc gcgaaatgcg aatggatggc cgtgccgggc aaccgcggtc ttcaccgctt
      601 caaggaatgc ggcgccggcg tgcgagagca tgactcgttc gcgatgaacc agccagaggc
      661 ggcgggcgat cttcagttcc ggaacggcaa cctgcaccag ctcgccccga gccagctccg
      721 gctggaccgt aagacccggc accagggcca cgccgttgcc gtaagcgacg aagcgcttga
      781 tcgcttccag actcggcagc tcgaccccca tgttgagggg agtttgatgg cgggcaaagg
      841 tctccaccac gcggtggcgg aggggcgagg ccaccgtgtg ggcgatgaag ttctccttgc
      901 ccagttcgcg caaagatacc tgcttccgcc gcgccagtgg atgcttcggg tcgaccacga
      961 aggtgaccga gtcggtgtaa acggcaatcg cgtgcaggcg cgcgtcgtcg gggcggaagg
     1021 agagaatgcc gaattcggcg gagcgttcga ggagctggtc ggggatctga ctggccaggg
     1081 atcgctgcac ggtgacgcca atgtgcggat agctggcacg gaacgcgtcc agcacaggca
     1141 gaagccagag gcaggtgtat tcgttggcgg cgatggtcag gcgcccgcgc tcgagcgagc
     1201 gcagctcttc cagcgccgtg atggtctcgc cgcgcaggcg cagcaggcgt tcggcgtaac
     1261 tgcgcaggag ctttcctgag gccgtcaggg tgccgtcgcg cgaggaacgc tcgaacaacg
     1321 gctcaccgac ctcctcttcg agcttgcgaa tcgcgtggct gatggccggc tgggtgcggc
     1381 gcatacgagc cgcggcccgg gaaaagccct tttcttcgac gacggcgagg aaggtctcaa
     1441 gctggtaaag gtccatgggg cggcgatcga tgcagtgcgc gcaatgatta tgaatcaaat
     1501 ttatgcagat gcaaagaatt acaaatttgc tttatggatg ggcctttgcc aaactaatta
     1561 cgtacggagc agcatggacg ctggagccgc gtgcggcgga tcgcaaagcg actcagcaaa
     1621 cgaggcactg tatgccccag aatcagattt acttcttcga tacgacgctg cgtgacggcg
     1681 agcagtcgcc
//
LOCUS       DASZYN010000828         3678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_829457, whole genome shotgun sequence.
ACCESSION   DASZYN010000828 DASZYN010000000
VERSION     DASZYN010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829457"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3012
                     /locus_tag="VHE33_20665"
     CDS             <1..3012
                     /locus_tag="VHE33_20665"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HVT99925.1"
                     /translation="FFMKMRSRLQLLLLACLPILAASSVHAQTAISSTYARDPQQPVD
                     QDYTDHIKKYTTEPYFNSPLTDYLPASKTVPTPAKVLGDVSGAPKILPYAEDVYKYFR
                     LLAASTPRVKVYTIGHSEEGREMIAAAIADESLLAKQKENDARLAKLADPRTIHLDDK
                     AAQDLVKQSFPIYYITGTIHSPETGAPTALMELAYRLAVDDSPYIKFIRSHMIVLITP
                     VVEADGRDRMVDIYKWHLAHPGQNYPRLVYWGHYVAHDNNRDAMGMTLNLTRNVLNTY
                     LTWHAQVLHDLHESVPFLYDNTVGDGPYNAWIDPILAGEWAEIGWNNVQQMTEFGMPG
                     VFTHGDFDTWSPGYLMFLAAMHNGISRLYETFGNGGADTEKRILQPDEYERTWYKQNP
                     PLPVVEWSQRNNNNYEETALLTTISYFAQHTDQWLENYYLKAKRSIEKPESSGPAAYV
                     ISADGQSPSRVAELLRVLALQHVEVSRLNEAVTVTNPAAKKKDEKSDAEKGDKDKSET
                     PKTLSFPAGSYVIRMDQPYSRIADALLDRQYWAPDDPQKHPYDDTGWSFPDLFAVPTT
                     RITDASILKAKMESVADPKAASGAVSGSGDWVAIENAGDISLLGLRYSLKDAKISVAD
                     QAFDAGGKHFGAGSLVIEGASADAVKSAIQNADLPAVSLASAPSVSMHDAPPPRIAMM
                     HTWAATQTEGWWREALDKLHIPYDYISTQTVSREADLRSKYDVILFAPTGGRGSTQQI
                     VNGMPMWGNPLPWQKTDLTPNLGRIDSTEDMRPGLGSAGVDHLKDFVEKGGLLIASED
                     TAQFAIDEGLAPGVFVNRGGNVKVVGSVLAAQFVDHKSPVAWGYGDHLAVYSADGMAF
                     TVGNMTVPRHIETEKDYHRATGRGGPQESDIPQGRAAVDPEPLPSPKPWEATPLNEEE
                     ARDNPYVIPEQYRPDVILRFGSSKGLLLSGLLEGGGAIEEKPIVVDAHYGQGNVLLFA
                     NNPIYRGETIGTYALVMNAILNFDHLAKAAK"
     gene            3034..>3678
                     /gene="lpxI"
                     /locus_tag="VHE33_20670"
     CDS             3034..>3678
                     /gene="lpxI"
                     /locus_tag="VHE33_20670"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037151.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HVT99926.1"
                     /translation="MDKLGLIAGNGRFPFLLLDAARAHGTEVVVAAIKEETDPEMNTR
                     AAADHGVRVHWLSLGELSRLIETFQQEGVHKAVMAGQVKHKQIFSSIRPDWRLAKLLL
                     NLRTRNTDMLLGAVAKVLGDEGIELISSTTYLEPLLAQPGVLTSRAPNDQEQRDIEYG
                     REVARGLAAWDLGQTVVIAAQACVAVEAMEGTDATVERAGALMRSLGDPPASTLE"
BASE COUNT          789 a         1239 c         1014 g          636 t
ORIGIN      
        1 ttcttcatga agatgcgtag ccgcctgcag ttgctcctcc ttgcctgcct ccccattctc
       61 gctgcgtctt cggttcacgc gcagaccgcc atcagctcca cctacgcgcg cgatccacag
      121 cagccggtcg atcaggacta caccgaccac attaagaagt acacgacgga gccgtacttc
      181 aattcgcctc tcactgacta tctgccggcg tcaaaaaccg tccccactcc ggcaaaagtc
      241 cttggcgacg tctcgggagc cccaaagatc ctgccctatg ccgaagacgt gtacaagtac
      301 ttccgcctgc tcgccgccag cacgccgcgc gtcaaggtct acaccatcgg ccattcggaa
      361 gaggggcgcg agatgatcgc cgcagccatt gccgacgagt cgctgctggc aaagcaaaaa
      421 gaaaacgatg ctcgtctcgc gaagctggcc gacccgcgca ccattcatct ggacgacaag
      481 gccgctcagg atctcgtgaa gcagtcgttt cccatctact acatcaccgg caccatccac
      541 tcgcccgaaa ccggcgcgcc taccgctctc atggagctcg cctaccgcct cgccgtcgac
      601 gattctccct acatcaaatt catccgctcg cacatgatcg tgctcatcac gcccgtcgtt
      661 gaggccgatg gccgcgatcg catggtggac atctacaagt ggcatctcgc ccatcccggc
      721 caaaactacc cgcgcctcgt ctactggggc cactacgttg cccacgataa caatcgcgac
      781 gccatgggga tgaccctcaa cctgacccgc aacgtgctga acacctacct gacctggcac
      841 gcgcaggtcc tccacgacct gcacgaatcg gtcccattcc tctacgacaa caccgtgggc
      901 gacggccctt acaacgcctg gatcgatccc atcctcgccg gcgagtgggc tgagatcggt
      961 tggaataacg tgcagcagat gaccgaattc ggcatgcccg gcgtcttcac ccatggcgat
     1021 ttcgatacgt ggagcccggg ctatctgatg tttctcgcgg ccatgcacaa cggcatcagt
     1081 cgcctctatg agaccttcgg caacggcggg gccgacaccg agaagcgcat cctccagcca
     1141 gacgagtacg agcgtacctg gtacaagcag aatccgcccc tgccggtggt cgagtggtcg
     1201 cagcgcaaca acaataacta cgaagagaca gcccttctga ccacaatttc ctattttgcg
     1261 cagcacaccg atcagtggct cgagaactac tacctcaagg caaagcgcag cattgaaaaa
     1321 ccagagtcct caggccccgc cgcgtatgtg atttccgccg atggccagtc tccgagccgc
     1381 gtcgcagaac tgctgcgggt cctcgcgctc cagcatgttg aggtcagccg gctgaatgaa
     1441 gcggtgaccg ttacaaatcc agcggcgaag aaaaaagacg aaaagagcga cgccgaaaaa
     1501 ggtgataagg acaagtccga gacaccaaag acgctttcct tcccggccgg aagctatgtc
     1561 atccgcatgg atcagcccta ctcgcggatt gcggacgcgc tgctcgatcg tcagtactgg
     1621 gccccagacg acccacaaaa gcatccctat gacgacaccg gctggtcatt ccccgatctc
     1681 tttgctgtac ccaccacccg cataacggat gcgtcgattt tgaaggcaaa gatggagtcg
     1741 gtagcggatc caaaggcggc gagcggcgca gtcagcggaa gtggcgattg ggtcgcgatc
     1801 gagaatgccg gagacatctc gctgctcggc ctccgctact cgctcaaaga cgccaaaatc
     1861 tcggtcgctg accaggcctt cgacgccggc ggcaagcact tcggcgccgg atcgctggtg
     1921 atcgagggtg cgagcgccga tgccgtaaaa tccgcgattc aaaacgccga cttgcccgcg
     1981 gtaagtctgg cgtctgcacc ctccgtttcc atgcacgatg cgccgccccc ccgcatcgcc
     2041 atgatgcaca cctgggccgc cacccaaacc gaaggctggt ggcgcgaggc gctcgacaag
     2101 ttgcacatcc cctacgacta catctccacc caaaccgtct cgcgcgaagc cgatcttcgc
     2161 tcgaagtacg acgtcatcct tttcgcgccg acgggcggtc gaggatcgac ccagcagatc
     2221 gtcaatggca tgcccatgtg gggcaatccg ctgccctggc agaagaccga tctgaccccg
     2281 aacctcggcc gcattgactc aaccgaagac atgcgcccgg gtctcggatc ggcaggagtc
     2341 gatcacctga aggatttcgt cgagaaaggc ggcctcctca tcgcctctga ggacaccgcg
     2401 cagtttgcca tcgacgaagg tctggcgcca ggcgtgttcg tgaatcgcgg cggcaacgtc
     2461 aaggtcgtcg gcagcgtgct cgcggcgcag tttgtcgatc acaagagccc cgtggcctgg
     2521 ggctacggcg accatctcgc cgtgtacagc gcggacggca tggctttcac cgtcggcaac
     2581 atgaccgtgc cacgccacat cgagaccgag aaggattatc accgcgcaac gggccgcggc
     2641 ggtccgcagg agtccgacat tccccagggc cgcgcggctg ttgatcccga gccgctgcct
     2701 tctccgaagc cctgggaggc aacgccattg aacgaggaag aagcgcgcga taatccgtat
     2761 gtgattccgg agcaataccg ccccgacgtg atcctgcgct tcggttcatc gaaaggcctg
     2821 ctcctgtcgg gcttgcttga aggcggcggc gcaatcgaag aaaaaccaat cgtcgtcgac
     2881 gcccactacg gccagggcaa cgtgctcctc ttcgccaaca acccgatcta tcgcggcgaa
     2941 accatcggaa cctatgcgct ggtaatgaat gcaatcctga acttcgatca cctggccaaa
     3001 gccgcaaagt agattcggga ccggaaagaa gtgatggaca aactcggact catcgccggc
     3061 aacggccgct tccccttcct cctgctcgat gcagcccgag cgcacggaac cgaagtcgtc
     3121 gttgccgcga tcaaagagga gaccgacccg gagatgaata cgcgcgccgc tgccgaccat
     3181 ggcgtgcgcg tgcactggct gtccctcgga gagttatccc ggctcatcga gaccttccag
     3241 caagagggcg tccataaagc tgtcatggcc ggccaggtga aacacaagca gattttctcc
     3301 agcatccgcc ctgactggcg tctcgcaaaa ctgctgctga acctgcgcac ccgcaacact
     3361 gacatgctgc tgggtgcggt cgcgaaggtg ctcggcgatg aaggcatcga gctcatctca
     3421 tccaccacct atctcgagcc gttgctcgcg caacccggcg tgcttacctc ccgtgcgccc
     3481 aacgaccagg aacagcgcga catcgaatat gggcgcgagg tcgcccgcgg tctggcggcc
     3541 tgggatctcg gacaaaccgt cgtcattgcc gcgcaggcat gcgttgccgt ggaagccatg
     3601 gaaggcaccg acgcaaccgt cgaacgagcc ggcgccctca tgcgcagtct gggagatccg
     3661 ccggcctcaa ccctcgaa
//
LOCUS       DASZYN010000829        10041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_435660, whole genome shotgun sequence.
ACCESSION   DASZYN010000829 DASZYN010000000
VERSION     DASZYN010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 10041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10041
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435660"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1583..2245)
                     /gene="tsf"
                     /locus_tag="VHE33_20675"
     CDS             complement(1583..2245)
                     /gene="tsf"
                     /locus_tag="VHE33_20675"
                     /inference="COORDINATES: protein motif:HMM:NF013085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HVT99927.1"
                     /translation="MSTVTEKIDAKLVKELREKSGAPMGDCLKALQETKGEIEEAFVV
                     LRKRGMASAAKKASRSTNEGQVGQYIHAGGKIGVIVEVNCESDFVARTDDFQELLKDI
                     AMHIAATDPRYIRKEDVTPEDLEREKEIYRAQTAALGKPANVVEKIVEGKMGKFYEEV
                     CLLEQPFIKEQSVTIKDLIATKVGKLGENITVRRFARFKVGDPTWTVAQTKAADETAA
                     EG"
     gene            complement(2393..3289)
                     /gene="rpsB"
                     /locus_tag="VHE33_20680"
     CDS             complement(2393..3289)
                     /gene="rpsB"
                     /locus_tag="VHE33_20680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HVT99928.1"
                     /translation="MATITMKELLEAGVHFGHQTKRWDPRMKEYIFGERNGIYIIDLQ
                     KTLKMFKEASKFVTELAASGKVILFVGTKRQAQDAIAEEATRCGMFYINQRWLGGLLT
                     NWVTVQKSVKRLQEIDEMANDGRYNLLTKKEVIRLERERKHLQANLAGIKNMRRLPDA
                     IFVVDSSNEAIAVKEARKLGIPVVAVVDTNCDPTVVDYVIPGNDDALRAIRLFTSKVA
                     DSVAEGVAAMSDKSIDQGGVTADIQAAGAEGEQAGEGAAAPADGEATEEVDLEAALGG
                     GIRKAPAIVAALDEAEAAESGY"
     gene            complement(3453..3848)
                     /gene="rpsI"
                     /locus_tag="VHE33_20685"
     CDS             complement(3453..3848)
                     /gene="rpsI"
                     /locus_tag="VHE33_20685"
                     /inference="COORDINATES: protein motif:HMM:NF001099.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HVT99929.1"
                     /translation="MADLVQYYGTGRRKSAIARVYLRPGSGEFKVNGKAFDTYFVTEQ
                     QRVSAKKSLVVSDTAATFDVVTTVSGGGVAAQADAVKMGIARALLQFNAELRKTLKSE
                     GLLTRDARVKERKKYGQKGARKRFQFSKR"
     gene            complement(3871..4305)
                     /gene="rplM"
                     /locus_tag="VHE33_20690"
     CDS             complement(3871..4305)
                     /gene="rplM"
                     /locus_tag="VHE33_20690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HVT99930.1"
                     /translation="MSTYIPSAHEIDRKWYVVDASGKTLGRLATEAASVLSGKRNPKY
                     TPFLDMGDHVIVINAGKVRLTGLKAQSKVYRRYTGFPGGLREESFVRLMERRPEKVVE
                     EAIRGMLPKTKLGRKMAAKLNVYRDEKHPHAAQKPQPLEVNA"
     gene            complement(4595..5434)
                     /locus_tag="VHE33_20695"
     CDS             complement(4595..5434)
                     /locus_tag="VHE33_20695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99931.1"
                     /translation="MKKSLIAALLGSAAFCSPVQPACAQQAFWEHFESRKVAMAGLQP
                     AWIAPLIESDPRLMQGVRLSVSNANAPGARIVSYGNNHGVGVIAGTRFQFDFNPPSFF
                     RNHSAELPDGWGNASTQVKFRIASGNASHGNFAVSAMVEHGFAPGASQKGILTSYNIT
                     KLAAGKAFGPFAVQSTLGGVLPTGRIEEQGRAVEWNIAGQFHVSSRTWLDIENNAAFL
                     LGGANDGKTQNFITPAAFLIMKRNGWGPRHPIAVLDGGMQIATSRFYCYNHNLITEVR
                     ILF"
     gene            5526..5984
                     /locus_tag="VHE33_20700"
     CDS             5526..5984
                     /locus_tag="VHE33_20700"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HVT99932.1"
                     /translation="MKRWFCAALLPLTLSLLSVRAQSTDHDRLVGAWKLLALEQPGPD
                     GKVNTRTCCGMFVFTRDGHLSVQVMEGDAKANAAPAGPQQYSQGGYEASYGSYVVDEK
                     NHIFTFHVDGALVKTLIGKDLPRRYTFEGDRLIVQSTEPDEHWRVVWIRY"
     gene            6115..6930
                     /locus_tag="VHE33_20705"
     CDS             6115..6930
                     /locus_tag="VHE33_20705"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HVT99933.1"
                     /translation="MRDTIMSVAEQKEPFGKPAAGSVFLHVGFVGLIAIMIFANDKFH
                     GNQWGVNGPQGTIQANLVNSAPSIPLPSITPPTPNVLATQTPSPAPAPPSKEVIPITP
                     PDAIPLAAHKPKLRPAEKPQPASPQHAQPTPAQNRAQYGEAASTNMAHAMSNAEARNN
                     APVQVQGGDFAARFPWYVDKITRLVSQNWYTYQIDSSTRYGSQVSITFTIHRDGSVDF
                     SSINLPQPSPSPSLNKTAREAVQRVGSFGPLPDQYTGKSVSVEYTFTYDQPAK"
     gene            7086..8444
                     /gene="tolB"
                     /locus_tag="VHE33_20710"
     CDS             7086..8444
                     /gene="tolB"
                     /locus_tag="VHE33_20710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HVT99934.1"
                     /translation="MIDLSPRNSVVLRTLLLLALTALPFSGAVAQDWVRTGTNLGVSK
                     IRIAAADFKAASADPQTGGLKSAFDQTLYNDLRYAGIFDVVSKSMIPTATPGSPQEIN
                     MTEWSGAPASAGYVAFGALNVSGGHLTVQGYLYNTAQGQQNPQVLGKQYGGESNAESA
                     RQIAHRFADEIIQQLGGIPGIAETKIYYVSNRSGNKEIWMMDYDGMNERQLTHLGTVS
                     LSPHVSPDNSRVAFSSLGKNGWSIRMYSIDLGRLMAFPAAGGTTLSPAWSSDGSKLAF
                     SSDRSGDPEIYVSDPSGNGARRLTAFHGPDVSPVWNPKTNAQIAWVSGRTRLPQIYIM
                     DSDGANITRMTDGGYATSPSWSPSGQFLAFAWNRSYGPGAPGGEDIYVMDIASKRWTQ
                     LTHEAGANDFPSWSPDSRHIVFQREQNGHSEIWMMLADGTDQQQLTHGGGSNTMPNWS
                     WK"
     gene            8544..9287
                     /gene="pal"
                     /locus_tag="VHE33_20715"
     CDS             8544..9287
                     /gene="pal"
                     /locus_tag="VHE33_20715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HVT99935.1"
                     /translation="MKYSNRTSLVMAATLAAMLTVAGCHKKKPAPPPPPPPPATAPQP
                     MATITAEPSSITPGGSATLTWKTTDATNVSIVGLGTVATSGTQTVNPTQTTSYHLVAQ
                     GDGGTADATATVTVTEAAPPPAPITETGIDENAFEAAVKPVFYDYDSYDVRPDAQSII
                     SADANFLNQHPNLKVVVGGYCDERGSTEYNLALGENRANAAKQALVSAGVSPDRLRTV
                     SYGKEKQFCSDHSEACWQQNRRAQFTLDQ"
     gene            9488..>10041
                     /locus_tag="VHE33_20720"
     CDS             9488..>10041
                     /locus_tag="VHE33_20720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99936.1"
                     /translation="MRKFVVPFSVVFLFVLFSAAPPAHAVNKEMVQLQTQVQQLQDAV
                     QHLQETQDQQLSVMQHIIQTINDNVSRMSSTVNTLQQQVQAQNESGGGKIEQVSGQMQ
                     GINDSIDALRTRIDKLSAEVQSVQSQLSNITTQAAVQQQGGQPGQPGADQNGQPGQPQ
                     AQQQPQAPPVSELYQGALRDYNSAR"
BASE COUNT         2060 a         3247 c         2799 g         1935 t
ORIGIN      
        1 cgaaacaacg tttcgatggg gtggcctgag agcggatccc cagcttccca ccattccacg
       61 ccagagatcc gtagagtacc ctttgggtaa cttccaacaa gctgctttgc caccaggaac
      121 atgatcgatg agctctttgg cgattaggcg ccaatcgaga tctggaaaag gctacttgtc
      181 cgacatggcg acgatacggc aaggtggatc gcggaattct gacccgtctg tgaggagtaa
      241 tccgcacacc atagagatac cgcttcagaa gtgcacagat cagccgatgt ttgatccgag
      301 gactaggctc gcgtcattac ttcctggcct aagcgccagt ccagaagata agcacgacta
      361 gaagaatcgt tgcaccactt gcgcagaggc cggtgatctt cagcttcccg ttagcggagc
      421 acgagaggat gaatgtaatt accgaaacta cagccgcaat catgaagaca tccgcgaaga
      481 cacccgcccg gcctgaaaaa tagtggccca ccgtccacgt tcgaaaactg gcttccagta
      541 gagccaagct tatgagcgtg atacccaata ccaccaacga cgctactgcg ccaaaaacgc
      601 ctgcaactga acgatgattg atagcgttcc tcaccgattg atggcttctt acatgcaaat
      661 cgataaaaca cgcactcttg ttccctgcag aatgaacgcc cgatccaaaa ttgtatatgg
      721 cgcagaatcg cgtctttgcg agcgtagagc tgagcccgcc agtgcatcac gggagattga
      781 tagagataac acggctagca tccatcgatc ccgtgtcccg tcaagttgtc gtaaagcggg
      841 catgtctgaa aagtgccact ggtcagtttt cgccaacagc ccgagtcccc tcatcgaaag
      901 cggtttgcgc cgcgatcagg ctagacaccg gacacccctc tccaactcag agcattcacc
      961 ctgagaatca aatcaggaaa accattacct tcgtgtccca caccaccaga aaatccttga
     1021 ttagctccca aacatccccc atcctgagag tggaagtgtg tattccaaaa aaggactcgc
     1081 ccaggcgagt ccttttcctt tggacctcag ggtccgccgg caccatccga gcaaacggga
     1141 ccggcccaga tctttgccat cgtcagcgag ctcgcccgca attccggcaa cgccgctcaa
     1201 tccgcccaac ccaagatgcc gtcaatcttt cgaggacaag cggtcgagcc ttaagggcct
     1261 cgggcaactg cttcggattg aattacccag tccccagggc cctcgacaaa ttcctgcacc
     1321 taatagcagc gcaaggactt ctgcccgccc ataacctgaa aaaaagagcc catgatttcg
     1381 gcccttacct cgaggccctc acgcttaacg ccgcggaata actgatcgga taacacggca
     1441 gggggtctcc ccggggcccc tccagaaaaa tcaacaactt cccgaggggt cgacccccct
     1501 cttttcaaaa aagcaaaagc ccggcatcgc tgccgggctc ctgcttgttg agtttttgcg
     1561 cgaagcgcga ttgccggggc ggctagccct cagcggccgt ctcatccgcc gccttggtct
     1621 gcgccacagt ccacgtcggg tcgcccacct tgaaccgcgc gaaccgccgc accgtgatgt
     1681 tctcgcccag cttgcccacc ttggtcgcga tcagatcctt aatcgtcacc gactgttcct
     1741 tgatgaaggg ctgctccagc aggcagacct cctcgtagaa cttgcccatc ttgccctcga
     1801 cgatcttctc caccacgttt gccggcttgc ccagcgccgc ggtttgggcg cggtagatct
     1861 ccttctcgcg ctccaggtcc tccggcgtca cgtcttcctt gcgaatgtag cgaggatcgg
     1921 tcgccgcgat gtgcatcgcg atgtccttca gcagctcctg gaagtcgtcc gtgcgcgcaa
     1981 cgaagtccga ctcgcagttg acctcgacga tcacgccgat tttgccgccg gcatgaatgt
     2041 actggccgac ctgcccctcg ttggtggagc gtgaagcctt cttggcggcc gacgccatgc
     2101 cgcgcttgcg cagcacaacg aacgcctctt caatctcgcc cttggtttcc tgcagcgcct
     2161 tgaggcagtc ccccatcggg gcgccggact tttcgcgaag ttccttgacg agttttgcgt
     2221 cgatcttttc agtcaccgtg gacatccttg ttactccatt ctttcagctt tcagaatttc
     2281 atgacccggt tacgcgaccg tcaacgcctg ccggatccgc attgcacaca aaaagcgtgg
     2341 ctcctgctga cggcagccac gctttcgtaa aaacgtacga ccgtcgaacg atctagtacc
     2401 cgctctcggc ggcctcggct tcgtccagag cagcaacaat cgccggcgcc ttgcggatgc
     2461 cgccacccag cgcggcttcc agatccacct cttcggtggc ctcgccgtcg gcgggcgctg
     2521 ccgcgccttc cccggcctgc tcgccctctg caccggccgc ctgaatatcg gccgtcaccc
     2581 cgccctggtc gatgctcttg tcactcatgg cggctacccc ttcggcaacc gagtcggcga
     2641 ctttcgacgt gaagagccgg atggctcgca gcgcgtcgtc gttgcccggg atgacatagt
     2701 caaccaccgt cggatcgcag ttcgtgtcga ccaccgcgac caccgggatg cccagcttgc
     2761 gggcttcctt caccgcgatc gcttcgttcg acgaatcgac cacgaagatc gcgtccggca
     2821 ggcggcgcat gttcttgatg ccggcgaggt tcgcctgcaa gtgcttgcgc tcgcgctcca
     2881 gccggatcac ttccttcttg gtgagcaggt tgtagcggcc gtcgttggcc atctcatcga
     2941 tttcctgaag gcgcttcacc gacttctgca ccgtcaccca gttcgtcagc agtccgccaa
     3001 gccagcgctg gttgatgtag aacatcccgc agcgggtggc ttcttcggcg atggcatcct
     3061 gcgcctggcg tttggtgccg acaaacagaa tcaccttgcc cgaagcggcg agttcggtca
     3121 cgaacttcga cgcctctttg aacatcttga gggtcttctg cagatcgatg atgtaaatcc
     3181 cgttgcgctc gccgaagatg tactccttca tcctcggatc ccagcgcttg gtctgatgcc
     3241 cgaagtgaac acccgcttcg agcagctcct tcatggtgat agtggccaaa cgacctcctc
     3301 tttggactgc attccggacg gtgctgccgg gccggaattg atgctcgccg cagcgagcag
     3361 atttgaacag caaactgagt tgtgagttat gagttccgca gtcgagagca aaagtcactc
     3421 accactcgca actcataact cgcccgaagc acttagcgct tcgagaactg gaaccgcttg
     3481 cgcgcaccct tctggccgta cttcttgcgt tccttcacgc gggcgtcgcg ggtgagcaat
     3541 ccttcgctct tgagggtctt gcgcagttcg gcattgaact gcagcagcgc gcgggcgatg
     3601 cccatcttca ccgcatcggc ctgggcggct actccgcccc cggaaacggt ggtgacgaca
     3661 tcgaacgtcg ctgccgtgtc gctgacgacg agcgatttct tcgccgagac ccgctgctgc
     3721 tcggtgacaa agtaggtgtc aaaagctttg ccgttgacct tgaattcgcc cgagccgggc
     3781 cggagataaa cacgcgcgat cgccgacttg cgacgtcccg tgccgtaata ctgaaccaga
     3841 tctgccatgc tgtgtgctct tcctcaatcc ttaggcgttg acttccagcg gctggggctt
     3901 ctgggccgcg tgcggatgct tctcgtcgcg ataaacgttc agcttggccg ccatcttgcg
     3961 gccgagcttg gtcttgggca gcatgcccct gatcgcctct tccacgacct tttcggggcg
     4021 gcgctccatc agccgcacaa acgattcttc tcgcagccca cccggaaacc cggtgtagcg
     4081 gcggtagacc ttggactgcg ccttgagacc ggtcagccgg actttgccgg cgttgatcac
     4141 gatgacgtgg tcgcccatat ccagaaaagg cgtgtacttc ggattgcgct tgccactcag
     4201 cacgctggca gcctcagtag caaggcggcc aagggttttg ccgctggcgt ccacgacgta
     4261 ccacttacgg tcaatttcgt gcgcgctcgg tatataagtc gacatctcgt tctcttctcc
     4321 cggcaaagcc gtcaatccca aaaattgtcc acgcaacgcc catccacgga actgaaactc
     4381 ttccgcggct tacgaatgct gtacgcgtga gttggcgcag aatatgaggc cccggtagcc
     4441 ggtcactgca agcttttccg gatcgaccat tcccggtctg cggcgcaaac gcacagacgc
     4501 ggaaacgcac actgcgcaag gacttcagga tacggtaatc ccgggataga gtcaatccac
     4561 aggcaatggc tgccggcagg ccgccggctg gcgctcagaa cagaatacgc acttccgtga
     4621 tcaggttgtg gttgtagcag tagaagcgcg acgtggcgat ctgcatcccg ccatcgagaa
     4681 cggcaatcgg atgcctcgga ccccacccgt tcctcttcat aatcaggaac gccgctggcg
     4741 tgatgaagtt ctgggtcttc ccatcgttag ccccgcccag caggaatgcg gcgttgtttt
     4801 cgatgtccag ccaggtccgg gagctcacgt ggaactgtcc ggctatattc cactcgacgg
     4861 cccgcccctg ctcttcgatc ctgccggtcg gcagcacgcc tcccagggtg gactgcacgg
     4921 cgaagggccc gaaggctttg cccgcagcca gtttcgtgat gttgtagctc gtcagtatcc
     4981 ccttttggct ggccccgggc gcaaatccgt gctccaccat cgccgagaca gcgaagtttc
     5041 catggctcgc attgccggac gcaatgcgaa acttcacctg cgtggacgca ttgccccatc
     5101 catccggcaa ctcggcgctg tggttccgga aaaacgaggg cggattgaaa tcgaactgga
     5161 acctcgtccc tgcgattacc ccgaccccgt gattgttgcc gtagctgacg atccgggctc
     5221 ctggcgcgtt cgcgtttgaa accgacaggc gcacgccctg catcaggcgc ggatccgatt
     5281 caatgagcgg ggcaatccac gcaggctgca acccggccat tgcgaccttg cggctttcaa
     5341 aatgctccca gaatgcctgc tgggcacagg ccggctggac cggcgaacag aaggcggcgc
     5401 taccgaggag ggctgcaatc agagatttct tcacgacttg gtgccaggat agctgcagag
     5461 tggcagcagg gtcagtgatt tacgccacac agctgggtag ctgaaaatga acggggagaa
     5521 tccgaatgaa gcgatggttt tgcgccgcgc tactgccgct taccttgagt ctgctttctg
     5581 tcagggctca gagcaccgac catgatcggc tcgtgggcgc atggaagctg ctggcgctgg
     5641 agcagcccgg ccccgatgga aaggtcaata caaggacgtg ttgcgggatg ttcgtgttta
     5701 ccagggatgg ccatctgtcc gtccaggtca tggaaggcga cgcaaaagcg aacgccgcac
     5761 ccgcaggacc gcaacagtac tctcagggtg gatacgaagc gtcctacggg agctacgtgg
     5821 tggacgaaaa gaaccacatc ttcaccttcc acgtcgatgg cgccctggtg aagactctga
     5881 tcgggaagga cctgccgcga cggtacacgt tcgagggaga tcgcctgatt gtccaatcaa
     5941 ccgagcccga tgagcattgg cgcgtcgttt ggatccgtta ctagacgcag atcggatcca
     6001 atgccggtac aacccaaagg tgtgctcccg ggttcctacc ggcggcgcgg ggttgtcgcc
     6061 aggggtccgg cggcgtcaaa ctagagaaag caccaaccgg cacaaaggaa gacgatgcgg
     6121 gacaccatca tgtcagtcgc ggagcaaaaa gagcccttcg ggaagcccgc ggcggggtct
     6181 gtctttctgc atgtgggatt cgtcggcctg atcgcgatta tgatcttcgc caacgacaag
     6241 ttccatggca accagtgggg cgtgaacggc ccgcagggaa ccatccaggc caacctggtc
     6301 aactctgccc cctccatccc cctgccgtcg attacaccgc cgacccccaa tgtgctggcc
     6361 acgcagactc ccagtccagc gccggctccg cccagcaaag aggtcattcc catcacgccg
     6421 ccggatgcga ttcccctggc cgcgcataag ccgaagctga ggcctgcaga gaaaccgcaa
     6481 cccgcttcgc cgcagcacgc ccagcccacc cccgcgcaga atcgtgccca atatggcgag
     6541 gccgcatcca cgaatatggc gcacgcgatg agcaatgccg aggcgcgcaa caacgccccc
     6601 gttcaggtcc agggcggcga ttttgctgcg cggttcccat ggtatgtgga caagatcacg
     6661 cgcctggtca gccagaactg gtacacctac cagatcgatt cgagcacccg ctatggcagc
     6721 caggtttcaa tcacgttcac catccaccgg gacggcagcg tcgatttctc cagcatcaat
     6781 ctgccccagc ccagtccttc gccttccctc aacaagactg cgcgcgaagc cgtgcaaaga
     6841 gtgggcagct tcggcccgtt acctgatcag tacacaggga aaagcgtttc cgtagagtac
     6901 acttttacct acgatcagcc ggcgaaatga ccgcgcgccg atcgtcttcg gatgtgccga
     6961 gttaccgatt tcccataccg gcagcgccat ccttccacgg ccgttccgct tcccatggga
     7021 cgaagacgga agcccccgat gcggagccgg caggccgtca tcaatcctat ggaaacggaa
     7081 gaagcatgat cgatctctcc ccccggaatt ctgttgttct tcgcactctc cttctgcttg
     7141 cgctcaccgc gctccccttt tccggagctg tagcccagga ctgggtacgg accggtacca
     7201 atctcggcgt gtcgaaaatt cgcatcgccg ccgcggattt caaggccgcc tccgccgatc
     7261 cgcagaccgg cggactgaaa tcggccttcg accagactct ctataacgac ctccgctacg
     7321 ccggtatttt cgatgtggtc tcgaagagca tgattccgac cgccaccccc ggctcgcccc
     7381 aggagatcaa catgaccgag tggtcgggcg cgcctgccag tgccggttat gtcgcgtttg
     7441 gcgcgctgaa tgtcagtggc ggccacttaa ccgtccaggg ctacctctac aacactgcgc
     7501 agggccagca gaatccccag gtgctgggca agcagtatgg tggcgagtcc aacgccgaat
     7561 ccgcccgcca aatcgcgcac cgtttcgccg acgagatcat tcaacaactg ggcgggattc
     7621 ccggcatcgc cgagacaaag atctactacg tctcgaaccg atcgggcaac aaagagatct
     7681 ggatgatgga ttacgacggc atgaacgagc gccagctgac ccacttaggc acggtctcgc
     7741 tctcgccgca cgtctctccc gacaattctc gtgtggcttt cagctcgctg ggcaagaacg
     7801 gctggtcgat ccggatgtac tcgatcgatc tcggacgcct gatggctttc cccgccgcgg
     7861 gtggaacaac cctctcccca gcctggtcaa gcgatggctc gaagctggcg ttctcctccg
     7921 atcgctccgg cgatcccgag atttatgtca gcgatccctc cggcaacggc gcgcgccggc
     7981 tgactgcgtt tcacgggccc gatgtctcgc cggtctggaa tcccaaaacc aatgcgcaga
     8041 ttgcctgggt cagcggtcgt acccggctgc cgcagatcta catcatggat tccgacggcg
     8101 ccaacatcac gcgcatgacc gatggcggat acgcgacctc gccctcgtgg tcccccagcg
     8161 ggcagtttct cgcctttgcc tggaatcgga gctatggtcc tggtgctccc ggcggcgagg
     8221 acatctatgt gatggacatc gccagcaagc gctggaccca gctcacgcat gaagccggcg
     8281 cgaatgactt tccgtcgtgg tcccccgaca gccggcacat tgttttccag cgcgaacaga
     8341 acgggcactc tgagatctgg atgatgctgg ctgacggcac cgaccagcag cagttaaccc
     8401 acggtggcgg gagcaacaca atgcccaatt ggagctggaa atgaccacct gatgaaaagc
     8461 ttgcagccgg gagttgttgc cggtcgttgt cattcttttg ccctggataa ccctcaaaac
     8521 gctccctgaa ggaggagaga caagtgaaat actccaatcg cacgtccctg gttatggccg
     8581 cgactctggc ggcaatgctg acggttgccg gatgccacaa gaagaagccc gcgcctccgc
     8641 cgccgccccc accgccggcc acagctccgc agccgatggc caccattacg gccgaaccca
     8701 gctccattac gccgggcggg agcgccacgc tgacgtggaa gaccacggac gcgacgaacg
     8761 tgtcgattgt gggcctgggc accgtggcga cctccggcac ccagaccgtt aacccgacgc
     8821 agaccaccag ctatcacctg gtcgcacagg gcgacggcgg aaccgccgac gccacagcaa
     8881 cggtcacggt gaccgaagcg gcgcctccgc ccgctccgat caccgaaacg ggcatcgatg
     8941 agaacgcgtt tgaagccgcg gtgaagccgg tgttctacga ctacgacagc tacgatgttc
     9001 gtccggacgc gcagtcgatc atctcggccg atgctaactt cctcaaccag catccgaacc
     9061 tgaaagtggt tgtaggcgga tactgcgacg agcgcggatc caccgagtac aacctcgcgc
     9121 tgggcgagaa tcgcgccaac gcggccaagc aggcgctggt cagtgcgggc gttagccccg
     9181 accgcctgcg caccgtgagc tacggcaagg aaaagcagtt ctgctccgac cacagcgaag
     9241 catgctggca gcagaaccgc cgcgcccagt tcacgctgga ccagtaaata cagggtacga
     9301 agcaaagggc tcagggcaca gcgagaggtt tctcgttgtg cagctgggcc ttttgctttt
     9361 gcctgcccga cggatcagga cgagaaaact ctggtaacga gctgagctct gagacctgag
     9421 ctgtgctctg acaccgcaac taattaataa tgaaggggag cgcttcctgc gctccgcggg
     9481 gattcttatg cgcaagtttg tcgttccgtt ctcagtcgtt ttcctcttcg tacttttcag
     9541 cgcggctccc ccagcccacg ccgtcaacaa ggaaatggtc cagctgcaaa cgcaggtaca
     9601 gcagctccag gatgcggtgc agcacctgca ggaaacccag gatcagcagc tcagcgtcat
     9661 gcagcacatc atccagacga tcaatgacaa cgtctcgagg atgagctcca ccgtgaacac
     9721 gctgcagcag caggtgcagg cgcagaatga atcgggcggc ggaaaaatcg agcaggtctc
     9781 cggacagatg cagggcatca acgactcgat cgacgcgctg cgcacgcgca tcgacaagct
     9841 cagcgctgag gtgcagtccg tccagtcgca gctttcgaac atcaccaccc aggcggccgt
     9901 acaacagcag ggcggtcagc caggccagcc tggagcagac cagaacggtc agccgggaca
     9961 gccgcaggcg cagcagcagc cgcaggcccc gcccgtcagc gagctctacc agggcgcgct
    10021 gcgcgactat aactcggcgc g
//
LOCUS       DASZYN010000830         2786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_60246, whole genome shotgun sequence.
ACCESSION   DASZYN010000830 DASZYN010000000
VERSION     DASZYN010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60246"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VHE33_20725"
     CDS             <1..365
                     /locus_tag="VHE33_20725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99937.1"
                     /translation="FADLPGYGYAKISKSISADWPKFIDPYLRERPTLSLCVCLVDST
                     IPPKASDAQLFEFLENAGRNYLIVGTKADRLSGNGRAKALAALRQAFGDVPLLFCSAK
                     TGAGVRELWASIRESVQS"
     gene            complement(499..888)
                     /locus_tag="VHE33_20730"
     CDS             complement(499..888)
                     /locus_tag="VHE33_20730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HVT99938.1"
                     /translation="MESLSSAEEVRPRVLIADDERIIADTLGMILNQNGFEAATVYNG
                     VKAVETARVFNPNIFLADVVMPGMSGIEAAIQICGMMPGCRVVLLSGQAATADLLRDA
                     RVRGHEFEILLKPVHPEQLLDRLRVAT"
     regulatory      1172..1416
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1467..>2786
                     /locus_tag="VHE33_20735"
     CDS             1467..>2786
                     /locus_tag="VHE33_20735"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HVT99939.1"
                     /translation="MTLCARPMGRFLTHLRLRLFALALLCAVPPGLHAVSVHGTITDP
                     LGYPIANATVALVQNGQVLLSARTGRDGGYAIVSPGSGRFYVLASGVSFRQLQTQSFY
                     GGRFDNVEQNVILEPETVRQSVVVTASGSPQPEAQVSASVSSLGVEDLMNHADVVNPL
                     RQIPGLNVVQQGQRGGVTSVFIRGGSSTASRVVMDGVPLEDIGGRFDFGTVATLGVQD
                     VEVYRGPNTVLYGSDAAAGVIALTTPRGSTSFPSFLYEGDAGSFGTYRNQAQLAGMMR
                     KLDYYVGASSLYTQNSIPNDQYHNGTAVSNLGWSWSAKTQVRVTMRGSNSATGLPGGN
                     GGYSFYGLANDGKQSDQDVYGTGTIAHAFRDNWHATVRYGLVRKREESGEWYPAGNLL
                     TTADLGAGNYYGNDVTVKGANGTASSGQALLNYGPIFGSVYPYKVYVA"
BASE COUNT          505 a          973 c          761 g          547 t
ORIGIN      
        1 tctttgccga tctgcctggc tacggatacg cgaagatttc caaatccatc tcggcggact
       61 ggcctaaatt catcgacccc tatctgcgcg aacggcccac gctctctctg tgtgtctgcc
      121 tcgtcgacag cacgatcccc ccgaaagcca gcgacgcgca gctctttgaa tttcttgaga
      181 acgcaggccg caactacctg atcgtgggca ctaaagcaga tcgcctctca ggaaacggcc
      241 gcgccaaagc actcgccgct ctgcgccagg cctttggaga cgttcctttg ctcttttgtt
      301 ccgcgaaaac cggcgccggc gtccgcgagc tctgggcctc gattcgggaa tccgtccagt
      361 cgtgaccggt cgagcgtccg cgtcctggtg tggtgtttct gaacttcctt tatcttgtca
      421 tcccgaccct gagcgtagcg aaggggaagc ggagggacct gcttctcttc cgcataaaga
      481 aattttggaa acaccacact aggtcgccac ccgcagccga tccaaaagct gctcgggatg
      541 aaccggcttc agcagaatct cgaactcatg cccgcggacg cgcgcatcgc gcaacagatc
      601 ggcagtggcg gcctggcctg acagcagcac cacccggcat cccggcatca tcccgcaaat
      661 ctggatggct gcctcgattc cgctcatccc cggcatgacg acgtcagcca gaaagatgtt
      721 gggattgaac acccgcgccg tctccaccgc tttcacgccg ttgtagaccg tagcggcttc
      781 gaagccgttc tgattcagga tcattccaag ggtgtcggcg atgatccgtt cgtcatcggc
      841 gatgagcact ctgggcctga cctcctcggc gctggagaga gattccattc gaccccttgc
      901 acggtcaaag acgttgaaat ttactgagaa ctgggattgt gtgctgcgag tatatccctg
      961 ctcaatccgg ccggtctcca aattttcttc atggaatgcg tgatctgcat ctggcgatat
     1021 tccaccgcct ccgctcatcc cagtccctcc tgtcgttcgc aatgccgatc atcaaccccc
     1081 tctttgactt gcgctgtcga acgatttata aggaggaatc cggacgcccc ccgttcggat
     1141 ccgctgcgaa aggccctgcg ggagtcgtcc cgccgtcgcg ttcgcgatcg atttccacca
     1201 gtgcaatggg atgttcggga aggcggtgaa aatccgccac tgccccgcaa ctgtaagcgc
     1261 gtgttcctgc gtctttgtca cgatgatgca gaacggccag gccagagcgc cactgaagtg
     1321 aaagcttcgg gaaggcggcc aggcttcgtt cttccggggt ccttgccgga aaatgccgcg
     1381 cgagtcagga gaccggtcct gcttgccgag cttgttgctg tccacaggct cactcaccgc
     1441 gttcccgcgg gggaacggag gagaccatga ctctttgcgc acggcccatg ggccgtttcc
     1501 tgacccatct tcgacttcgc ctttttgcgc tcgcgctctt gtgcgccgtg ccgccgggcc
     1561 ttcacgccgt cagcgtccac ggaaccatca ccgatccgct cggctacccc atcgccaacg
     1621 ccaccgttgc cctggtgcag aacggccagg tgctgctcag cgcccgcacc ggccgcgacg
     1681 gcggctacgc catcgtcagt cccggctccg gccgctttta cgtgctcgcc tccggcgtct
     1741 cgttccgtca gctgcaaacg caaagcttct acggcggccg cttcgacaat gtcgagcaga
     1801 acgtcatcct cgagccggaa accgttcgcc agtccgtcgt cgtcacggcc agcggctctc
     1861 cgcaacccga agcccaggtc agcgcctccg tctccagtct cggcgtcgaa gatctcatga
     1921 atcacgccga cgtcgtcaac cccctgcgcc agatccccgg cctcaacgtg gtgcagcaag
     1981 gccagcgcgg cggcgtcact tcggttttca tccgcggcgg ctcctcgacc gcaagccgtg
     2041 tcgtgatgga tggcgtgccc ctcgaagaca tcggcggccg ttttgacttc ggtaccgtcg
     2101 ccacgctggg cgtccaggac gttgaggtct atcgcggccc caataccgtg ctttacggat
     2161 ctgatgcagc cgccggtgtc atcgcgctca ccacgccgcg cggcagcacc tcgttcccgt
     2221 cttttcttta cgaaggcgac gccggcagct tcggcaccta ccgcaatcag gcgcagctcg
     2281 ccggcatgat gcgcaagctt gactactacg tcggcgcctc cagcctctac acacagaact
     2341 ccattccgaa cgaccagtac cacaacggca ccgccgtctc gaacctcggc tggagctggt
     2401 cggccaaaac ccaggtccgc gtcactatgc gcggaagcaa ctccgccacc ggtctgcccg
     2461 gcggcaacgg cggctactcg ttctacggcc tcgccaacga cggcaaacag agcgatcagg
     2521 atgtctacgg caccggcacc atcgcccacg cgttccgcga caactggcac gccaccgttc
     2581 gctacggcct cgtccgcaag cgcgaagaat ccggcgagtg gtaccccgct ggcaatctgc
     2641 tcaccaccgc ggatctcgga gcagggaact actacggcaa cgacgtcacc gtgaagggcg
     2701 ccaacggaac cgcttcatcc ggccaggccc tgctcaacta cggccccatc ttcggctccg
     2761 tctaccccta caaggtctac gtcgcc
//
LOCUS       DASZYN010000831         7215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_60271, whole genome shotgun sequence.
ACCESSION   DASZYN010000831 DASZYN010000000
VERSION     DASZYN010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60271"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VHE33_20740"
     CDS             <1..691
                     /locus_tag="VHE33_20740"
                     /inference="COORDINATES: protein motif:HMM:NF024096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HVT99940.1"
                     /translation="TKRAFDFGHAAHRFVLGEGEDIVPVDADDWRTKAAKEQAESARA
                     EGKVPLLRAEFDRALAMANALQDNPDAAALFKSGRAEHAMYATDTKTGVRLRGRADWL
                     TETNGQLVIVDYKTAASADPDTFERASTKYKYYLQAAWYRELVIACGLSDDPDFVLVA
                     QEKQPPYLSSVMRFDDIAIAEGRALKRQAVTTYARCLQTGEWPGYPSGTQIISVSPWA
                     YSEDDIEMKAV"
     gene            694..1752
                     /locus_tag="VHE33_20745"
     CDS             694..1752
                     /locus_tag="VHE33_20745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HVT99941.1"
                     /translation="MTVYYTDDQVTLHHGDALEVARELPNAAADCIVTSPPYFGLRDY
                     GTSGQYGLESSPAEYVETMRGLFTELRRVLADDGTLWLNIGDSYVAGASMTGTKSMRQ
                     RLNGSHLRNDTISRGTSDLPPKNLLGIPWRVAFALQDDGWILRNAIIWHKPNAMPESV
                     TDRLSGRYEHVFLFSKSRRYWFDLDAIREPHGLRELSEGGRNPGDVWTLPTQPFSAAH
                     FAVYPIQLPQRCILAGCKLRRCQECGHAPEPIIERNLRQRDLRPDPEGEAKGVPPDEV
                     IPLKNKRAPSGTYTDCGHNAYRAGTVLDPFSGSGTTGLAAQRTGRKYIGIDLNVDYLE
                     LSLKTRLTNSTLNFEEPA"
     gene            1752..2327
                     /locus_tag="VHE33_20750"
     CDS             1752..2327
                     /locus_tag="VHE33_20750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99942.1"
                     /translation="MDISATTVPDSTQVNAEDFLASPRTVTITGVSKGTAEQPVNIDL
                     AEFPGRAFRPCKSMRRVLIAAWGSDADAYIGRRMTLFCDPSIRFGSQVVGGIRISHLS
                     HIDHKLTLALTTTRGKRAPFVVEPLADAPPPISDEDAADFARCIAESSTVAELDSVAT
                     ALKSCDLGTHRKRLQSAWAERKAALETEAAK"
     gene            2817..3617
                     /locus_tag="VHE33_20755"
     CDS             2817..3617
                     /locus_tag="VHE33_20755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99943.1"
                     /translation="MANAAALIRESLWKDKDFRQLPRLAQCMYLQLLSQKDLDCSGVL
                     TLHLELLAKGCNEMTVEDILADLKTLEEARYVFVDIDTDELLIRSYVRLVSVKSPNAW
                     KSVLKSARLVESEKIRRELAAELRRLNRKDAIDLANEIDPGPNPSEPHSKPIPNPSEG
                     DNPSERGSEPPSSVLVVGHLTSSSSQVGEAPPICPKHPENSDAPCPACKRRRLWETTQ
                     RERQERDELEQRRRLREIRDNCPDCNGTNLIDLGDEVRKCDHSKVRNA"
     gene            3630..3809
                     /locus_tag="VHE33_20760"
     CDS             3630..3809
                     /locus_tag="VHE33_20760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99944.1"
                     /translation="MLPNWTGTRARADALRVPCRYCGAQVGEPCVTKDLDPRPLEAFP
                     AHVVRTNDAQKATEV"
     gene            3806..4126
                     /locus_tag="VHE33_20765"
     CDS             3806..4126
                     /locus_tag="VHE33_20765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99945.1"
                     /translation="MTAPDLESIAGLDWTEYACNCPCHPQGCDNRATHVVSIHALHRC
                     NEPGLTFGDRVEFRCVECLLRLQAEVHFRLEEVNRWGVGECGSCGAPMTRMTDLVRDW
                     SPLA"
     gene            4123..4437
                     /locus_tag="VHE33_20770"
     CDS             4123..4437
                     /locus_tag="VHE33_20770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99946.1"
                     /translation="MRDTRTPQKSLYGVTQTQQSLSLIQCSGCQNGWTGMSACHCSGC
                     HRTFTGITAFDIHRTGGRCNEPAELLTDKGEPRLVAVDKLYWSGWGLPGERPAGDLTW
                     TP"
     gene            4425..4850
                     /locus_tag="VHE33_20775"
     CDS             4425..4850
                     /locus_tag="VHE33_20775"
                     /inference="COORDINATES: protein motif:HMM:NF014520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HVT99947.1"
                     /translation="MDPIGALRAIANRETWQERALCSQTDPELFFPGKGQSPRHAKTI
                     CRQCDVRLECLDYALRNNERFGVWGGTTDYERSRRNGGMPKPTRRPPDECVNGHKLEN
                     PDTDSSHNCRQCAREKYERYMARVRAGLVEGRRPRDGVA"
     gene            5355..5630
                     /locus_tag="VHE33_20780"
     CDS             5355..5630
                     /locus_tag="VHE33_20780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99948.1"
                     /translation="MKLLPMIDKRRRVRLWDKDWQCTHDLRIQPDETPFGVVSEIMRE
                     QLLVEVRATVDDEHGDRVAAIFYRDSDGAVQFVSYDEIRKLFEWPKP"
     gene            5627..6127
                     /locus_tag="VHE33_20785"
     CDS             5627..6127
                     /locus_tag="VHE33_20785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99949.1"
                     /translation="MNTIAMAAWLSRNIPAIACDEASGELFRDVQNAVEAIERMINRP
                     IPDRVLGPCPTLVAHHQECATQLTAPRHATEIVCPACDQTHSTEQLTERLFRQMEYRT
                     FTIRELLDVVLPRLEEWVPRSTLYFWVAKKKLTSSSSNAEGEPLYLLSDVRKLRSAQP
                     RTRQTS"
     gene            6382..6537
                     /locus_tag="VHE33_20790"
     CDS             6382..6537
                     /locus_tag="VHE33_20790"
                     /inference="COORDINATES: protein motif:HMM:NF040003.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6131 family protein"
                     /protein_id="HVT99950.1"
                     /translation="MLVLGLVLFLVGYFCAIGILETIGIILLVIGAVLFMVGAVGHPI
                     GGRRYWY"
     gene            6537..6668
                     /locus_tag="VHE33_20795"
     CDS             6537..6668
                     /locus_tag="VHE33_20795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99951.1"
                     /translation="MIDDRWETWGIAARWDAQHGWHDPVNDIGFPEHLLKDMDDATA"
     gene            6848..>7215
                     /locus_tag="VHE33_20800"
     CDS             6848..>7215
                     /locus_tag="VHE33_20800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99952.1"
                     /translation="MNELTYFTVEGHWYDVEAPDTSGSTNALQFLVISAFVTFTPRLE
                     PGFSLFIPNLDLQAQLVAPSSLAVAPSTTGGTLAAGATYWVVTATTANGETTVSNEVT
                     ATLTGSTSSAVLSWSAVVGAT"
BASE COUNT         1569 a         2274 c         2084 g         1288 t
ORIGIN      
        1 cactaagcgc gccttcgact tcggccacgc tgcgcatagg ttcgtcctcg gtgaaggtga
       61 agacatcgtg cccgtggatg ctgacgactg gcgcaccaaa gccgcgaagg agcaagccga
      121 gtccgcacgc gccgagggga aagtgccgct gctgcgtgct gagttcgaca gggcgctcgc
      181 tatggctaac gcgctgcagg acaaccccga cgccgcggcg ctgtttaagt ctggccgcgc
      241 cgaacacgct atgtatgcca ccgacactaa aacaggggta cgcttacggg gcagggctga
      301 ttggctcact gagaccaacg gccagttggt catcgtggac tataaaacgg cagcgagtgc
      361 ggaccccgac acatttgaac gcgcgtccac gaaatacaag tactacctcc aagcggcctg
      421 gtaccgcgag ctcgttattg cctgcgggct cagtgacgat cctgactttg tgcttgtggc
      481 acaagagaag cagccgccgt acttgtcgtc ggtgatgcgt ttcgatgaca tcgccatcgc
      541 cgaaggtcgc gcgcttaaac gccaagctgt cacaacctac gcgcgatgcc tacagaccgg
      601 cgaatggccc ggatacccaa gcggcacaca gattatctcc gtctcgccgt gggcctattc
      661 agaggatgac atcgaaatga aggcggtctg acgttgactg tctactacac cgacgaccaa
      721 gtcacgctgc accacggcga cgcgctcgag gtcgcccgcg aactacctaa cgctgcagcg
      781 gattgcatcg tcaccagccc gccctatttc ggtttgcggg attatgggac atctggccag
      841 tacgggctgg aatcctcgcc ggccgagtac gtcgagacga tgcgtggact gttcaccgag
      901 ctgcgacggg tgctggctga tgacggcacg ttgtggctca atatcggcga cagctacgtt
      961 gccggcgcaa gcatgaccgg aaccaagtca atgcggcagc ggctcaacgg ttcacatcta
     1021 cgcaacgaca ccattagtcg cggcacttcg gatctaccgc ccaagaacct cctcggcatc
     1081 ccctggcgcg tcgcattcgc cctgcaagac gacggctgga ttctgcgcaa cgccattatt
     1141 tggcacaaac ccaacgccat gccggaaagc gtcaccgacc ggctcagcgg ccggtacgag
     1201 catgtgttcc tgttcagcaa gtcgcggcgg tactggttcg atttggatgc gatacgggaa
     1261 ccgcatgggc tgcgggaact atcggaaggt ggccgcaacc ccggcgatgt ctggacgctc
     1321 cccacgcaac ctttcagcgc cgcgcacttc gccgtctacc ccatccaact cccgcaacgc
     1381 tgcatcctcg ccggctgcaa actgcggcgc tgccaggaat gcgggcacgc gccagagccg
     1441 atcatcgaga gaaatctccg ccaacgtgat ttacgcccgg acccggaggg ggaggccaag
     1501 ggggtccccc ccgacgaggt aatcccactg aagaacaagc gggcaccatc cggcacatac
     1561 accgactgcg ggcacaacgc ataccgcgct ggaaccgtcc tcgacccctt ctcgggcagc
     1621 ggcacaaccg gactcgcagc gcaacgcacc gggcgcaaat acatcggcat cgacctgaat
     1681 gtcgactacc tcgagctgtc gctgaagacc cggttaacca actcaacgct taacttcgag
     1741 gagcctgcct aatggacatt tcagcaacaa ccgtgcccga cagcacgcaa gtcaacgccg
     1801 aggacttcct cgccagcccg cgcacagtaa ccatcaccgg cgtcagcaag ggaaccgccg
     1861 agcagcccgt gaacatcgac ctggccgagt tccctggccg cgcattccgc ccctgtaaat
     1921 caatgaggcg cgtccttatt gctgcctggg gcagtgacgc tgacgcctac atcggtcggc
     1981 gaatgacctt gttctgcgac ccgtcaatcc ggttcggatc ccaagtcgtg ggcggcattc
     2041 gcatcagcca tctctcgcac atcgaccaca agctcaccct ggcgctgacg acgacaagag
     2101 gtaagagagc gccgtttgtt gtcgagccac tggctgatgc gccaccaccc atcagcgacg
     2161 aggacgcagc cgacttcgca cgctgcatcg ccgaatcgtc caccgttgcg gaactggatt
     2221 cggtcgccac tgcgctcaaa agttgcgacc tggggacaca tcgaaagcgg ctgcagagtg
     2281 cctgggctga gcgcaaggcg gcgcttgaaa cggaggccgc gaagtgaatg cgaacagcct
     2341 cgcgaatcta tcagtcgcga atcagaagcg gaccaaggaa gggtcgccgc gcattgtctg
     2401 gctagcccaa ctcgcgctag accatcccga gcgtttaacc cccgctgagc accgcgccgc
     2461 caaggcgcgc gttgaccatc ctgagctaaa cctgtcacaa atcgcggaag tcctgggcac
     2521 ctccaagagc tccctttggt cacgcctacg gactgcgatc cgaaaggtta atgccggcgg
     2581 tacttggtgc ggcagctgcc agtcccgcat cccactgagc cacaatcgct gcccgcggtg
     2641 ccgcgcatga gcatcttcga cgacctcgca cgccgcaaca gccccgcagt caaggctgct
     2701 ttgcgcgaaa aatgccccgt ttgtaaggcg attccgggcg aagattgcac caatttacct
     2761 attaatggat ttccacttca aaatcggcta gttcactatg cgagggctac aggctaatgg
     2821 ctaatgcagc cgcacttatc cgcgaatcac tctggaaaga caaagacttc cgccagctcc
     2881 cacgcctggc gcaatgcatg tatctgcagt tgctgtcaca aaaggacctc gactgctcgg
     2941 gtgtgctgac ccttcacctc gagctactcg ccaagggctg caatgagatg accgtagagg
     3001 acatcctggc cgacctcaaa accctcgagg aagcccgcta cgtcttcgtc gacattgaca
     3061 ccgacgaact actcatccgc tcgtatgtgc ggttggtcag tgttaagtct ccgaacgcct
     3121 ggaagtcggt tctcaagtcc gcacggctag tggaatcaga gaaaatccgc cgtgagttgg
     3181 ccgccgagct gcgtcggctg aaccgcaagg atgccatcga cctagcaaac gaaatagacc
     3241 ctggtccgaa cccctctgaa ccccattcca aacccattcc aaacccatcc gaaggggata
     3301 acccatccga aaggggttcg gaacccccca gttcagtact agtagtaggt catctcacct
     3361 ctagtagttc tcaggtaggg gaggcccccc caatttgtcc aaagcatcca gagaactcag
     3421 acgcgccctg ccccgcctgt aagcgtcgac ggctctggga aacaactcaa cgcgaacgcc
     3481 aagagcgcga cgagctcgag cagcgacggc gtctacgcga aatccgtgac aactgcccgg
     3541 actgcaacgg gaccaatctg attgacctcg gcgacgaggt tcgtaaatgc gaccactcca
     3601 aagttcgcaa tgcgtgaggg agcctgacga tgctgcctaa ctggaccggg acccgcgctc
     3661 gcgctgacgc gctgcgcgtc ccatgccgct actgcggcgc ccaagtcggc gagccctgcg
     3721 tcaccaaaga cctcgacccg cggcccctcg aagccttccc cgcacatgtc gtgcggacca
     3781 acgatgcaca gaaagcaacc gaagtatgac cgccccagac ctcgaatcca tcgccggcct
     3841 cgactggacc gaatacgcct gcaactgccc atgccatccg cagggctgcg acaaccgcgc
     3901 cacgcacgtc gtctcgattc acgcgctgca ccgctgcaac gagcccgggc tgacgttcgg
     3961 cgaccgcgtg gagttccgct gcgtcgaatg cctgttgcgg ttacaggccg aggttcactt
     4021 ccggctcgag gaagtcaacc gctggggtgt cggcgagtgc ggctcgtgtg gggcgccgat
     4081 gacgcggatg acggatttgg tgcgcgattg gagcccgttg gcatgaggga caccagaacg
     4141 ccacaaaagt ctctctatgg cgtcacccag acccagcaat cgctatccct gattcagtgc
     4201 agcgggtgcc agaacggttg gacgggcatg agcgcctgtc actgcagcgg atgccaccgc
     4261 acctttaccg gcatcacggc gttcgacatc caccgaactg gcggccgatg caacgagccg
     4321 gccgagctac tcaccgataa aggcgagccg cgcctagtgg cggtcgacaa gctgtattgg
     4381 tcaggctggg gactcccagg cgaacggccg gcaggggacc tcacatggac cccataggcg
     4441 cactgagggc catcgccaac cgcgaaacct ggcaggaacg cgcgttgtgt tcgcaaaccg
     4501 accccgagtt gttctttccc ggcaagggcc agagcccacg ccacgcgaag accatctgcc
     4561 gccagtgcga cgtccgtcta gagtgcctcg actacgccct gcgcaacaac gaacgcttcg
     4621 gcgtttgggg cggcaccaca gactacgaac gcagccggcg caacggtgga atgcccaagc
     4681 cgacgcgacg cccacccgac gagtgcgtca acgggcacaa actcgagaac ccagacaccg
     4741 acagctcgca caactgccgg caatgcgcga gggagaaata cgagcgttat atggcgcggg
     4801 tgcgtgcggg attggttgaa gggcgacgac ctagggatgg tgtggcgtga aggcaatgcg
     4861 catcgaccag aattatttcc gaatcacaga tggcgagaaa tggtggtcgg cgagcgcgct
     4921 cacgagcggc tggttaattg ttaaccgtgt ggggcgcgtg ataaagcagc gtggggatct
     4981 aggtaggcgc attattgctg cgattgaaga agtctcatga gcggaaccca atgccaacgc
     5041 tgcgcccgca aaacccagct attcctctgc caagcgtgcg ccacagacct caaggacctc
     5101 ctcgacaaca tggtctcgcg caccgcaacc aacctcatca caggcgagtc acgtcccgcg
     5161 gccggctgga ttgaactgct tgaggaccac gcacacgggc agacgcggct gggtgagtct
     5221 gcgcgcaggt ccaccgaacg caatacgccg ttgcccgtgc atctcggggc gtcgcagttg
     5281 ttggagaatg tgaggaacat gttgtcaacg tgggtgaggc atttgtgtga gtcgagaggg
     5341 attgaggtgc cggaatgaaa ctactgccca tgattgacaa acgtcgccgc gtgcggctct
     5401 gggataaaga ctggcaatgt acccacgatt tacgcatcca gcctgacgaa acgccgttcg
     5461 gtgttgtctc ggagattatg cgcgaacaac tactcgttga ggttcgcgcc accgtcgatg
     5521 atgaacatgg ggaccgggta gcggccatct tctaccgcga ctcagacgga gcagtgcagt
     5581 tcgtcagcta tgacgaaata cgcaaactat tcgaatggcc caagccatga acaccatcgc
     5641 catggccgcc tggctctctc gcaacatccc tgccattgcc tgtgacgagg cgtccggcga
     5701 gctctttcgt gacgtgcaga acgctgtgga ggcgattgag cggatgatta accggccgat
     5761 accagaccgc gtgctaggcc catgtccaac actggttgcc catcaccagg aatgcgcaac
     5821 gcagctcacc gcgccacgtc atgccactga aatcgtgtgc ccggcttgcg accagacgca
     5881 cagtactgag cagttgacag agcggctgtt tcggcagatg gaataccgca cgttcaccat
     5941 ccgcgagttg ctggatgtgg tgctgccacg cttggaggaa tgggtgcctc gctcaacgct
     6001 gtacttctgg gtggctaaga agaaactcac ctcgagctcg tcgaacgccg agggtgagcc
     6061 gctgtatttg ctgtcggatg tgcggaagct gcggagtgcg cagccgagga cgagacagac
     6121 aagttgaatg acaacggtgt gattatggtt tagattggac agcatcctcc cctagcacaa
     6181 ctgtgtctag gggatttttc atgccttgaa gtgaggcgac atgtccgcgc cagcacaccc
     6241 tgtctccgcg cagctcgtaa ccgacttgag cagcacgtta gcgcggttac gcatggcccg
     6301 catggttggt gacgagcctg aagccagggt tgcccaacgc cggctcgatt ggttgtgtga
     6361 caaactaccg aggagtaaat cgtgttagtt ctcggcctgg tgctgttcct cgtcggatac
     6421 ttctgcgcca tcggcatcct tgagacaatc ggcatcatct tgcttgtcat cggtgcggtg
     6481 ttgttcatgg tcggtgccgt cgggcatccc attggcgggc gcaggtattg gtactgatga
     6541 ttgacgaccg ctgggaaacc tggggtatcg ccgcccgctg ggatgcacag cacggctggc
     6601 acgacccggt caatgacatc ggattccctg agcatctact gaaggatatg gacgatgcca
     6661 cggcctaaag ccacaccaga gccagcgact gccgaacccg taccacctgg acctcaccag
     6721 ctcgaggtgc gtgtcgacgg caaaatcaag ttcctgcagc gcgtccacag ttatcacatc
     6781 gaccagcaac cacaccaagc ggtgatcacc ggcgcgctgc ggccaactat taaggtaccc
     6841 cgtgacagtg aatgagctga cctacttcac tgtcgagggc cactggtacg acgtcgaagc
     6901 ccccgatacc agcggcagca ccaacgcact gcaattcctg gttatcagcg cattcgtcac
     6961 tttcacgccg cggctcgagc ccggatttag cctgttcatt ccgaacctgg atctacaagc
     7021 ccagctcgtc gcgcctagct cacttgctgt agctccatca acaactggtg gcacattagc
     7081 ggccggcgca acctactggg tggtcaccgc aacaacggcc aatggtgaga cgaccgtatc
     7141 caacgaagtg acagccacac tcacgggcag cacatcgtcg gcagtcctgt catggtccgc
     7201 tgttgtcggc gcgac
//
LOCUS       DASZYN010000832         1970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_756296, whole genome shotgun sequence.
ACCESSION   DASZYN010000832 DASZYN010000000
VERSION     DASZYN010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756296"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VHE33_20805"
     CDS             <1..1146
                     /locus_tag="VHE33_20805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99953.1"
                     /translation="HYRVAALPWIAKAALPQIRWQDGRTIVSMPNGHLVGKVGSYGDE
                     LTAYLRFDYLKGIESIGGSRLFLVTTEEEGGPRYTLWVEMPNDVLEASETLAGLVIAR
                     HIDRFELESPPDVEMAAWEKQTKLFDAAYQDPVREKLLQLPRGALTSAVASFILFKVR
                     TDRRIRERLEPAAGKELSIDDARNFAADMIAVADFYEIPLDMLLGIGAMENNYLDIRG
                     DLQHAVWKRHAQRGDIVLRRRRRRVLVSNYSLGPWQITRETLRYAHRLYLRDKRDYSR
                     LPERLRPPRELDLERIDTDVLTTYAGLLIRDLLDYFHGDVARAVGAYNGGRKQPNPEY
                     STGVAMVADYARRVVGMAAGRKGNAVSETAVVVEQNGEAGVPETDRR"
     gene            1560..>1970
                     /locus_tag="VHE33_20810"
     CDS             1560..>1970
                     /locus_tag="VHE33_20810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVT99954.1"
                     /translation="MQGNDHSVIRAVLAGDKDAYGVLVRAHSAVVFRVAFRIVGNEAD
                     AEEIVQEAFLRGYQRLESFEQRSSFGTWIYRIAANCALNRVGKQEIKADYRHGEESDP
                     EEKTVQLAAREAGPERELLSREIQAAQEMAMRRLT"
BASE COUNT          388 a          488 c          746 g          348 t
ORIGIN      
        1 cattatcggg tggctgcgct gccgtggatt gcgaaggcgg cgcttccgca aatccggtgg
       61 caggatggac gaacgatcgt cagcatgccc aatggacatc tggtggggaa ggtgggcagc
      121 tacggcgacg aactgacggc gtacctgcga ttcgattatc tgaaaggaat cgagagcatt
      181 ggcgggtcgc ggctttttct ggtgaccacg gaagaagagg gagggccgcg ttacacgctg
      241 tgggtggaga tgcccaacga tgttctggag gcgtccgaga cgctcgccgg gctggtgatt
      301 gcgcggcaca tcgacaggtt cgagctggaa tcgcccccgg atgtggagat ggcagcatgg
      361 gagaagcaaa cgaagctctt cgatgcggca taccaggacc cggtgcggga gaagctgttg
      421 cagctgccgc ggggagcgct gacgtcggcg gtggcgagct tcatcctgtt caaggtgcga
      481 accgaccggc ggatccggga aaggctggag ccggcagcgg gcaaggagct gtcgatcgac
      541 gatgcgagga atttcgccgc cgatatgatt gccgtcgccg atttctacga gatcccgctg
      601 gacatgctgc tgggcattgg ggcgatggag aacaactacc tggatatccg cggggatctg
      661 cagcatgcgg tgtggaagag gcatgcgcaa cgcggggata ttgttctgcg gcggaggcgg
      721 cggcgtgtgc tggtgagcaa ctactccctt ggtccctggc agatcacgcg agagacgctc
      781 cgttacgcgc accggctgta tttgcgggac aagcgcgact acagccggtt gccggagcgg
      841 ctgcgtccgc cgcgtgaact ggatctggag cggatcgaca ccgatgttct gacgacgtac
      901 gcaggtctgc tcattcgcga cctgctggat tactttcatg gagacgtggc gagggcggtg
      961 ggggcgtaca acgggggacg gaagcagccg aatccggagt attcgacggg agtggcgatg
     1021 gtggccgatt atgcgcgccg tgtggtggga atggcggcag gccgcaaggg caacgcggtc
     1081 tcggagacgg ctgtggtggt ggagcagaac ggggaggcag gggttcccga aacggatcgc
     1141 cgctgaggga agaacgttac gctcgtgcgg gagcggaacg gattgcgatt gattttgagc
     1201 ggaaaagggc gctcgcttct tgctatgggg tgaacctggc gtcttcaagc cggcggcgct
     1261 tcgcaggaat tgaccacgcc agggaattgg cggccgcgac caacactttg cagccgcggg
     1321 cagctacgcg tcgggggcaa actacgcagc cgcgggcaaa ctacgcagtc gcgggcaaac
     1381 tacgcagtcg cgggcaaact acgcggtcga gcacttcgcg gcaatatgga agcggagcga
     1441 gggcgctggc gtctgatgct gaatggcgcc ctgacggcga ggtctgcgca tcttctactg
     1501 atcgcacaag ggaaaatagt ttcctaaccg cgagtcctgg gggcggtcca aaggtttgca
     1561 tgcagggaaa tgatcactcg gtgatccggg cggtactggc cggcgataag gacgcgtacg
     1621 gcgtgctggt tcgtgcgcac agtgcggttg tgttccgagt ggcgtttcgc atcgtcggga
     1681 acgaggccga tgcggaggag attgtgcagg aagcgtttct gcgtggatat cagcggctgg
     1741 agagtttcga gcagcgctcg tcgttcggga cgtggattta tcggatcgca gcaaactgcg
     1801 cgctgaaccg ggtgggtaag caggagatta aggccgatta ccggcacggt gaagagagcg
     1861 atccggaaga gaagaccgtg cagctggcgg cgcgcgaggc agggccggaa cgggagttgt
     1921 tgagccgcga gatccaggcg gcgcaggaga tggcgatgcg gagactgacg
//
LOCUS       DASZYN010000833         6403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_319076, whole genome shotgun sequence.
ACCESSION   DASZYN010000833 DASZYN010000000
VERSION     DASZYN010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319076"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VHE33_20815"
     CDS             <1..879
                     /locus_tag="VHE33_20815"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HVT99955.1"
                     /translation="VAPLRFFNFVSPGYFHAMGTRILAGRDYNWDDLYGARPVAIVSE
                     SFAREEWGSTAAAMGKGIGQALNKPWLRVIGVVEDVRQNGVDHPAPAMVYWTAMFEVG
                     YLSKRTVAVIRSVTFAVHSGNAGTEAFLNEVQQAVWSVNGNLPVASPETMQQIYDGSM
                     ARTSFTLVMLAIAGSMALLLGIVGIYGVIAYAVAQRRREIGIRLALGAQRRELRWMFV
                     RSALLLTSVGIGLGVVAAAGLTQLMKSLLFGISPIDPVTYAAVVALLGGVAMVASYVP
                     ARRAAAVNPVEALRAE"
     gene            complement(887..1297)
                     /locus_tag="VHE33_20820"
     CDS             complement(887..1297)
                     /locus_tag="VHE33_20820"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HVT99956.1"
                     /translation="MPSPSAAIDQKIQQLGDWRGETLARIRSLIHEADPAIVEEWKWA
                     KATNPGTPVFSHAGIVCTGETYKSAVKLTFAKGASLKDPARLFNSSLEGNVRRAIDLH
                     EGDRINEAAFKELIRAAVDLNVQGKSKSSRTANR"
     gene            complement(1347..1859)
                     /locus_tag="VHE33_20825"
     CDS             complement(1347..1859)
                     /locus_tag="VHE33_20825"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HVT99957.1"
                     /translation="MSATYTPGPATGAEVQKQGEKWTLILVRELRHTPDHVWQALTDP
                     AQLREWAPFEVDGDLETVGAIVHLTWAGTGRVSEATVRCAERPRRLEFADIRWQLDPS
                     DAGTRLTLWHSIDRRYVTWGAAGWHICFDVLDRLLAGQPIGRRIGPDAMRLEAWQRLT
                     AEYAKQFDSD"
     gene            complement(1878..2276)
                     /locus_tag="VHE33_20830"
     CDS             complement(1878..2276)
                     /locus_tag="VHE33_20830"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HVT99958.1"
                     /translation="MKIKITTLYVDDQEKALQFYTEVLGFVKKSDFSQGSYRWLTVAS
                     PEDPDGTELQLALNNNPAAKTFQQALFQQNQPAAMFFTEDLNADYERMQSHGAQFTMP
                     PTDVTASKIAMLNDTCGNLVQITQLMVWKG"
     gene            complement(2273..2659)
                     /locus_tag="VHE33_20835"
     CDS             complement(2273..2659)
                     /locus_tag="VHE33_20835"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HVT99959.1"
                     /translation="MQSAFEIVAEPNRRAILGLLASSRQSVGDIERQLRLPQPAVSKH
                     LRVLRDAGFVESTVDAQRRLYSLNPKPLQELDAWLEQFRRFWSPHIDALERHLDRMHP
                     STPHNSNERKHRAATPPVPAKKRGKR"
     gene            2804..3256
                     /locus_tag="VHE33_20840"
     CDS             2804..3256
                     /locus_tag="VHE33_20840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HVT99960.1"
                     /translation="MTPKDFRRIALSFAGVQQVAHVGLPAFRTEGGRFASLASQAEGY
                     GNLVLTPEQQAAFVEEAPEVFLPIPGGFGRMGHTHIRLAVANEEMLTGALRTAWKLRI
                     EKTQRKRRHAEIGTGSRPISRRGGRYCGFAEGAVSAATRFVVGFRGLG"
     gene            complement(3184..4350)
                     /locus_tag="VHE33_20845"
     CDS             complement(3184..4350)
                     /locus_tag="VHE33_20845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVT99961.1"
                     /translation="MRPCGYLRFLLCVLLAAAPVSLLAQAVTQPAQTPQPAPVTLPNP
                     QVPESQPSLDVDHDPIVSPDAADNAAVSPSHPNPNSAGQEIKRNQGVYTLTRDVDEVV
                     LACTVVDQDGHLVQDLNRDDFHVFEDNSPQQIVAYSHSDVPVSLGLLVDNSGSMRDKR
                     SAVNTAALDLVRASNPQDEAFVVNFSDEAFLDQDFTADINKLRGGLSHIESRGGTALY
                     DAVVASADELSQGAKRSKQVLLIITDGEDNASTLNLEQTVRRVQDLGGPVIYSIGLLF
                     GDEDRGQESRRAKRALQLLSDETGGLAFFPKSLADVDTIAGEVARDIRNQYTIGYHSS
                     KPASLGGYRTVRVEARAPHHNKLFVRTRTGYYPKPLKPTTKRVAAETAPSAKPQ"
     gene            complement(4478..4843)
                     /locus_tag="VHE33_20850"
     CDS             complement(4478..4843)
                     /locus_tag="VHE33_20850"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HVT99962.1"
                     /translation="MAIAYLVVAVLLAAMTFFSGVGKLRHDPSIVKAIHETCRIPMRY
                     FPFLAACEMAGATGVVLGIWWPPLGVAGAAGLVLYFLGAVASHLRVGDSRGIGPAAFL
                     LSLSVAALVLRLFQHGSAL"
     gene            5184..>6403
                     /locus_tag="VHE33_20855"
     CDS             5184..>6403
                     /locus_tag="VHE33_20855"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HVT99963.1"
                     /translation="MVSWSLARFLAVFLTSFFAAPLAARAWASSSLAPVSGGVDTKQL
                     KTLSLEQLGNIEVVTQSKEPTEVWNTPAAIYVLTAEDIRRAGVTNVPDALRLVPGVNV
                     ARVSGDRNWLVAIRGLGDQYSKYVLVLIDGRSVYTPLFGGVYWPIDNVMLEDIDRIEV
                     IRGPGGSIWGADAVNGIINIITKSSKATHGVLASAGGGNVDENTEDLRYGSHSHGFDF
                     RVNGFGFVRGAEYHQNGQPNYDWSRLAQVGFHADRAAGPDTVTLQGDAYLGHLGDAQT
                     ISTFTPVATYISYASTNVYGGNLLGRWRRELPASADLYLQAFWSHDHRVGPNFGETRD
                     TFDVDFLHRTPPTRYQQFTYGFGGRWSPSRTLVSTPTDRFEPASETESVYSGFLQEEV
                     KVVPNRLSLTLGSKL"
BASE COUNT         1183 a         1775 c         2197 g         1248 t
ORIGIN      
        1 gtggcgccgc tgcggttctt caacttcgtg tcgccgggtt acttccacgc gatgggcacg
       61 cggatcctgg ccgggcgcga ttacaactgg gacgaccttt acggcgcgcg gccggtagcc
      121 atcgtgtcgg agtccttcgc ccgcgaggaa tggggatcga ctgccgccgc catgggcaaa
      181 gggatcggac aggcattgaa taagccatgg ctgcgcgtaa ttggcgtggt ggaagacgtc
      241 cggcagaacg gcgtcgatca tcctgctccg gcaatggtgt attggacggc gatgtttgag
      301 gtgggatatc tctcgaagcg gacggtcgcc gtgattcggt ctgtgacgtt tgcggtgcac
      361 agtgggaacg cgggtaccga ggcatttctg aacgaggtgc agcaggcggt gtggtcggtg
      421 aacggcaacc tgccggtggc ttctccggag accatgcaac agatctatga cggctcgatg
      481 gcgcgcacat ccttcacgct ggtcatgctg gcgatcgcgg gatcgatggc gctgctgctg
      541 ggcatcgtcg gaatttatgg agtcatcgcg tatgcggtcg cgcaacggcg gcgcgagatc
      601 ggtattcggc tggcgctggg cgcgcaacgt cgcgaactgc gctggatgtt tgttcggtcg
      661 gcgctgctgt tgaccagtgt cggaatcggc ctcggagtgg tcgccgcggc tggcctgacc
      721 cagttgatga agtcactgct gttcgggatc agcccgatag atccggtgac ctatgcggcg
      781 gtggttgcgc tgctcggcgg ggtagccatg gtagccagct acgtgcccgc acgacgcgcg
      841 gcggcagtga atccggtcga agcactgcgg gcggagtaaa aacgcgctac ctgttggctg
      901 tccggcttga tttggatttg ccctggacgt tgagatcgac agcggcgcga atcagctcct
      961 tgaaagccgc ttcgttgatc cggtcgcctt cgtgaaggtc gatggcacgc cgaacattgc
     1021 cttcgagact cgaattgaaa agacgggcgg gatctttgag agacgcccct ttggcgaaag
     1081 tcagcttgac cgcgctctta taggtctcgc cggtgcagac gatcccggcg tgggagaaga
     1141 cgggcgtgcc cgggtttgtg gctttggccc acttccattc ttcgacgatt gcagggtcgg
     1201 cctcatgaat gaggctgcga attctggcga gggtttcgcc acgccagtcg ccgagttgtt
     1261 ggatcttttg gtcgatcgcc gccgagggtg atggcattcg atgctccttc tcggaaatta
     1321 tcaatcgtcg ttgaacattg agcgcgttag tcggagtcga actgctttgc atattcggcc
     1381 gtgagccgct gccacgcttc gagccgcatc gcgtccggcc cgatacgccg gccgatgggc
     1441 tggccggcga gcaggcggtc gagaacatcg aagcaaatgt gccagcccgc agctccccag
     1501 gtgacatagc ggcgatcgat gctgtgccag agggtcaggc gagtgccggc gtccgaaggg
     1561 tcaagttgcc agcggatgtc ggcgaactcg agccggcgag gacgctcggc gcatctcacg
     1621 gtagcttccg agacacgccc ggttccagcc caggtgagat ggacgatggc gccaacggtt
     1681 tcgaggtctc catcgacttc aaacggcgcc cactcccgta actgcgcggg atccgtgagc
     1741 gcctgccaga catggtccgg tgtatggcga agctcgcgga cgaggatcag ggtccacttc
     1801 tctccttgct tttgtacctc ggctccggtg gcaggtcctg gtgtgtaggt cgcagacatc
     1861 gaattgctcc tgaaatttca gcccttccac accatgagct gcgtgatctg gacgagatta
     1921 ccgcaggtat cgttgagcat ggcgatcttc gaggccgtga cgtcggtggg cggcatcgtg
     1981 aactgcgcgc cgtgcgactg catgcgctcg tagtcggcgt tcaggtcctc ggtgaaaaac
     2041 atggccgccg gctggttctg ctggaaaagg gcctgctgga atgttttcgc cgcggggttg
     2101 ttgttgagcg caagctgcag ttcggtgccg tcgggatcct cgggcgacgc gacagtcagc
     2161 cagcgatacg agccctggct gaagtcggac tttttcacga agcccaggac ttcggtgtag
     2221 aactgaagcg ccttttcctg atcgtcaacg tagagggtgg tgatcttaat cttcatcgct
     2281 ttcctcgttt tttcgccggg acaggcggag tcgcggcccg gtgtttcctt tcgttgctgt
     2341 tgtgtggggt ggagggatgc atgcggtcga ggtggcgttc gagcgcgtcg atgtgcgggg
     2401 accagaagcg gcggaactgc tcgagccacg cgtccagttc ctgcagaggt ttcggattga
     2461 ggctgtagag gcggcgttgt gcgtcgaccg tggattcaac gaatccggcg tcgcgcagca
     2521 cgcgcagatg cttggagacg gcgggctggg gcagccgaag ctggcgctcg atgtcgccca
     2581 cagactgccg ggaggaagcc agcagaccga gaatcgcgcg gcggttgggc tcggcaacga
     2641 tttcgaaagc agactgcatg ttctgtatat acccgagtgg gaatatacgt gtcaaggcat
     2701 atagaaaaat ccttcaacac gcccaactgc ctgaactccg ccgggcgcac ggcaggctcg
     2761 tcggcgctct ttgatgggat actcgtcgct acgaggtgta gggatgactc cgaaggattt
     2821 ccggaggatt gcattgagct tcgcaggcgt gcagcaggtt gctcatgtcg ggctgcccgc
     2881 gtttcgtacc gagggcggca gatttgcgtc gctggcatcg caggccgagg gctatggaaa
     2941 ccttgtgctc acgccggaac agcaggcggc gtttgtcgaa gaggctccgg aggtttttct
     3001 gcccatcccc ggcggtttcg ggcggatggg ccatacccac attcgcctgg ctgtagccaa
     3061 cgaggagatg ctgacgggcg cactgcgaac cgcatggaag ctgaggatcg agaagacgca
     3121 aagaaaaaga agacacgcgg agatcggaac aggtagtcgg ccgatctcgc ggcgtggcgg
     3181 ccgttactgc ggctttgccg aaggcgcggt ttccgcggcg actcgcttcg tcgtcggctt
     3241 cagaggcttg gggtagtaac cggtacgcgt gcgaacgaag agcttgttgt gatgcggagc
     3301 cctggcttcg acgcgcacgg tccggtagcc gcccaggctg gctggcttcg aggagtgata
     3361 gccgatggtg tactgattgc ggatgtcgcg ggcgacttcg ccggcaatcg tgtcgacgtc
     3421 agccagtgac ttggggaaga acgcgagccc gccggtttcg tcggagagaa gctggagagc
     3481 acgtttggcg cgccgggatt cctggccgcg gtcctcgtcg ccgaaaagca gaccgatgga
     3541 gtagatcaca gggcccccga ggtcctggac acgccgcacc gtctgttcga ggttgagcgt
     3601 ggaagcgttg tcctcgccgt cggtgatgat gagaagaacc tgttttgacc gcttggcgcc
     3661 ctgggacaat tcatccgccg aagccacgac ggcgtcgtac agcgcggtgc cgccacggga
     3721 ttcaatgtgg gagagcccgc cccggagctt gttgatgtcg gcggtgaaat cctgatcgag
     3781 aaaggcctcg tcagaaaaat tgaccacgaa tgcttcgtcc tgagggttgg aggcgcggac
     3841 caggtcgagc gccgcggtgt tcactgccga gcgcttgtcg cgcatcgagc cggagttgtc
     3901 cacgagcagg ccgagggaaa cgggaacatc ggagtgcgag taggcgacaa tctgctgcgg
     3961 gctgttgtct tcgaagacgt gaaagtcgtc ccggtttagg tcctgcacga ggtgtccgtc
     4021 ctgatcgacg accgtgcacg cgagcaccac ttcatcgacg tcgcgagtca gggtatagac
     4081 accctggttg cgcttgatct cctggcccgc cgagttggga ttggggtgcg acggactcac
     4141 agcggcgttg tcggcggcat cgggtgaaac gatcgggtcg tggtccacat cgagggacgg
     4201 ctgggactcg ggtacctgcg gattcggcag ggtcaccggc gcgggctgcg gtgtctgtgc
     4261 gggctgggtc acggcctgcg cgagcaggct aacgggggca gcagcgagga ggacgcagag
     4321 gaggaatcga aggtaaccgc aggggcgcat agagtccggc acgtgcatgt acatatagtt
     4381 gcggccaggc gagtcggggc tgcaacctaa tcttcaattt acgccaattc gggggtacac
     4441 ggggttcggg gagcggcttc ggggcaaccg gcagaaccta aagggccgag ccgtgctgaa
     4501 aaagccgcaa aaccagggcg gccacagaga gggaaagcag gaaggccgcg ggtccgatac
     4561 cgcgactgtc cccgacgcgc aggtgggagg ccaccgcgcc cagaaaatag aggaccagac
     4621 cggctgcgcc tgccacgccg agcggcggcc accagatgcc gagaacgaca cccgtggcgc
     4681 cggccatttc gcaagccgca aggaacggaa agtagcgcat cggtatgcgg caggtttcat
     4741 ggatggcttt gacgatggag gggtcgtggc gcagcttgcc cactccggag aagaaggtca
     4801 ttgccgccag caacacggcc accaccagat acgcaattgc catggaggac cctttcgggg
     4861 gcaatcctat cgagcacaca gcgttcccgg ccatagccac cgtcggctcg tgcgacaggg
     4921 ggaacatggc cagccaggag ccggcggcgc tgaaggtgtg agggataccg atgatgctgc
     4981 ccgctgcggg ttaacagctg gcacgaaagg gggataacgg atttgccagg aacagcgggt
     5041 ggcgtcgtat agtgaatgtc gtattgcgac gggttccgcg gggacaccca gatccccgga
     5101 aacgaagaag acccgctgga tgcatgcccg ccgcgagctg tactcgctgt tcttcccaga
     5161 tttcctggtg cacggagcgt gcgttggttt cctggtccct tgcccgcttt cttgcggtgt
     5221 ttcttacctc cttttttgcg gcgccgctgg cagcgcgggc gtgggcttcg tcgtctctcg
     5281 ccccggtgag cggaggagta gatacgaagc aactgaagac gctctccctg gaacaactgg
     5341 gcaatatcga agtcgtcacg cagtcaaagg agccgaccga ggtttggaac accccggcgg
     5401 ccatctatgt gctgaccgcc gaagacatcc ggcgagccgg agtaaccaat gttcccgatg
     5461 cgctgcggct ggttcccgga gtcaatgtgg cgcgggtgag cggcgaccgc aactggctgg
     5521 tggccattcg cggcctgggc gatcaatatt cgaaatatgt gctggtcctg attgacggac
     5581 gcagcgtcta caccccgttg ttcggtggcg tctactggcc gatcgacaac gtcatgctgg
     5641 aggacattga tcggatcgag gtgatccggg gtccgggcgg cagcatctgg ggcgcggacg
     5701 ccgtgaacgg gatcatcaac atcatcacga aatcgtccaa agccacccat ggcgtgctgg
     5761 cgtcggcagg cggcggcaat gtggacgaga acaccgagga tctgcgctac ggcagccaca
     5821 gccacgggtt tgatttccgc gtgaatggat ttggattcgt gcgaggcgcc gaataccacc
     5881 agaacggaca gccgaactat gactggtcgc gcctggcgca ggtgggattt cacgcggacc
     5941 gcgccgcggg tccggacacg gtgaccttgc agggcgatgc ctatcttggg catctgggag
     6001 atgcacaaac gatctccacg tttacgccgg tagcgaccta tatttcgtat gcctcgacca
     6061 atgtctacgg aggcaatctg ctggggcggt ggcggcgcga actgcctgca agtgcggatc
     6121 tgtatctcca ggcgttctgg tcccacgatc accgggtcgg gccgaacttc ggagagacgc
     6181 gcgatacgtt cgacgtcgat tttttgcatc gcactccgcc gacacgatac cagcagttca
     6241 cctacggctt tggcggacgc tggagcccca gccggaccct tgtctcaaca ccgacagacc
     6301 gatttgagcc ggcctcggag accgaaagcg tctacagcgg ctttttgcag gaggaagtga
     6361 aggttgttcc aaaccggctg agcctcacgc tgggatcgaa gct
//
LOCUS       DASZYN010000834         2599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_756559, whole genome shotgun sequence.
ACCESSION   DASZYN010000834 DASZYN010000000
VERSION     DASZYN010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756559"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VHE33_20860"
     CDS             <1..229
                     /locus_tag="VHE33_20860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99964.1"
                     /translation="YYDLKKRVVERTTLAQPGAGDTAADGFVELNAAALAMPCQCTIE
                     FEKPCGSRLRIELQGSIPDLEALGRSFWESR"
     gene            229..591
                     /gene="tnpB"
                     /locus_tag="VHE33_20865"
     CDS             229..591
                     /gene="tnpB"
                     /locus_tag="VHE33_20865"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HVT99965.1"
                     /translation="MLQITPQMRIFVAVEAVDFRNGIDGLVRVCKEVLRQDPFQGGVF
                     VFRNRRATALKALVYDGQGFWLCHKRLSEGHFRWWPSATAGDKAKTLAAHQLHVLLHA
                     GDPESTKAAPDWRPVLSA"
     gene            717..2078
                     /locus_tag="VHE33_20870"
     CDS             717..2078
                     /locus_tag="VHE33_20870"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HVT99966.1"
                     /translation="MSVIDKDADVAVRWSTAERVQALKRIVGKKSIEKVCWQSPRQRY
                     CRRLPRWFVVWFVIALALFCRDSYRQVFRCLHRFRPGGTPGRSTLCEARKSIGVACLR
                     RLAEAVIELLARPDTPRTHYAGLRLMALDSFVVDVPDTPANERAFGRPQSGRAPGAFP
                     QARVLSLCEIGTHVLWRSLIKPHRRGEIPMARYLVRFLQANMLLLWDRNFLSYDLVQS
                     VRQRKAHLLARIKKNLVFIPFKQLPDGSYLAKLYPSPRHRQRDEEGIVVRIIDYTLDD
                     PGRPGSGQKHRLLTTLLSASRHPAMGLIDRYHERWEEELTIDELKTHQRERPVLRSET
                     PAGVVQEIYGLLLGHFVIRKLAFEAAATISCPPREISFVGTLKILRCRLPEAPRNRTS
                     LAQWYANLVAEVTEERLPPRRDRINPRVIKRKMSGWAKKTNKHRDSPQPTKNFRQSIV
                     LVN"
     gene            complement(2311..>2599)
                     /locus_tag="VHE33_20875"
     CDS             complement(2311..>2599)
                     /locus_tag="VHE33_20875"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HVT99967.1"
                     /translation="TTVPQQALFAMNSPFVMAQARRLAARPEVVAEDDPARRVQALYQ
                     LVFGRRANDDELDLALRFIASPQASSEASKLSPWELYAQILLSTNEFLFVD"
BASE COUNT          531 a          795 c          772 g          501 t
ORIGIN      
        1 ctactatgac ctcaaaaaac gggtcgtgga aagaaccact ctcgcccaac caggtgcggg
       61 cgacacggcg gccgatggct tcgtcgaatt gaatgcagcg gcgctggcca tgccctgcca
      121 gtgcacgatt gaattcgaga aaccgtgcgg ttcgcgactg cgaatcgaac tccagggaag
      181 cattcccgat ctggaggcac tcggccgcag cttctgggaa tcgcgctgat gctgcagatc
      241 actccccaga tgcggatctt cgtagccgtc gaagcagtcg atttccgcaa cggcatcgac
      301 ggcctggtgc gggtgtgcaa agaggtgctg cggcaagatc cgtttcaggg cggcgtgttt
      361 gtctttcgca atcggcgggc aaccgccttg aaggcgctgg tttatgacgg tcagggattt
      421 tggctttgcc acaagcggct ttccgaaggg cactttcgct ggtggccctc cgcgaccgcc
      481 ggagacaaag cgaagacgct cgccgcccat caactgcacg tgctgctaca cgcgggcgat
      541 cccgagagca ccaaggcggc ccccgactgg cgaccggtcc tgtccgcctg agcagccgag
      601 ccggagcgcg aaactcgtct tgggttaatc ccaataccgt tcaattgatc taagagaatt
      661 cgcagcctcc tgcgacgatg gggatgtcat aagtcccacg tagcaaggag gctgcgatga
      721 gtgtgatcga caaggatgcc gatgtcgccg tgcggtggtc gacagcggaa cgcgtccagg
      781 cattgaagcg gatcgtcggc aaaaagtcca tcgagaaagt atgctggcag tcgccacgtc
      841 agcggtactg tcggcggttg ccacgctggt tcgtggtctg gtttgtgatc gctctggcgc
      901 tattttgtcg tgactcatat cggcaggtgt ttcgctgcct gcaccgtttc cgcccaggag
      961 gcacaccagg acgctcgacg ttgtgcgaag cacgcaagag catcggcgtc gcctgtctgc
     1021 ggcgactggc cgaggccgtg atcgaactgc tcgcccgtcc cgacacgcca cggacgcact
     1081 acgccggtct gcggctgatg gcattggaca gtttcgtggt cgatgtgccg gacacaccag
     1141 cgaacgaacg agcctttggg cggcctcaaa gtggccgcgc gcctggggct tttccgcaag
     1201 cccgagtgct cagcttgtgc gagattggca cgcacgtgct gtggcgctcg ctgatcaagc
     1261 cgcatcgccg cggagaaatt cccatggctc gctatctcgt gcggtttttg caggcaaata
     1321 tgctgctgtt gtgggaccgt aacttcctca gctacgactt ggtccagagc gtgcgccaac
     1381 ggaaagcgca tctgctggcc cgcatcaaaa agaacctcgt tttcattccc ttcaagcaac
     1441 tgcccgacgg ttcttacctg gcgaagctct atccctcgcc acggcaccgc caacgcgacg
     1501 aagagggcat cgtcgtgcgg attatcgact ataccctcga cgatccgggg cggcctggct
     1561 ccgggcagaa gcatcgtcta cttacgaccc tgcttagcgc gtcgcggcac cccgccatgg
     1621 ggctcattga tcggtatcac gaacggtggg aagaagagtt gaccatcgac gaactgaaga
     1681 cacatcagcg cgaacgtcct gtcctacgaa gcgaaacgcc ggcaggagtc gtccaggaaa
     1741 tctatggcct cctattgggg cacttcgtga ttcgtaagtt ggccttcgaa gcagccgcca
     1801 ccatctcctg tccgccccga gaaatctctt tcgtgggcac gctcaagatt ctccgctgcc
     1861 gcctgccgga ggctccacgc aaccgcacca gcctcgccca gtggtacgca aacctcgtcg
     1921 ccgaagtcac cgaagagcgt ctgccgccac gtcgcgatcg aatcaacccg cgcgtgatca
     1981 aacgtaaaat gagtggctgg gccaagaaaa ccaacaaaca ccgtgattct ccccaaccca
     2041 caaagaactt ccgtcaatcc atcgtcctgg tgaattgaac ggtattgggg ttagcgtagt
     2101 ttttggcgct tccaccggaa gttaggtggg ctgggaagtg cgagggacaa actaccacac
     2161 gctgttccgg agcgctggct caaccagtgg ctgcgacaag ctcagcacct ttgctcactc
     2221 cccaggtcaa ctggcccggg gcagcgaaaa aacaaatccc cttatgcacc cacgggcgtg
     2281 tgatcgcgcc ggttgatgct cgccgaaaat tcagtccacg aacagaaact cgttcgtgct
     2341 caacaagatc tgggcgtaca actcccacgg cgaaagtttt gacgcttcgc tcgatgcctg
     2401 tggggaggcg atgaaccgca acgcgagatc gagctcgtcg tcgttagcgc gacgcccgaa
     2461 gacaagctgg tagagcgcct gcacgcgccg cgcgggatcg tcctcggcca ctacttcggg
     2521 tctggctgcc aaacgccgtg cctgcgccat cacgaagggc gagttcatgg cgaacagcgc
     2581 ctgctgcgga acggtcgtt
//
LOCUS       DASZYN010000835         3158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_512273, whole genome shotgun sequence.
ACCESSION   DASZYN010000835 DASZYN010000000
VERSION     DASZYN010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512273"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..1431)
                     /locus_tag="VHE33_20880"
     CDS             complement(196..1431)
                     /locus_tag="VHE33_20880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004022858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HVT99968.1"
                     /translation="MAEAFSAETLDSLIKDAVKSGTPIDGAEGLLNELTKAVLERSLQ
                     TEMTHHLGYEAGDPAGRGSGNSRNGSTSKTVNTVNGPVEINVPRDRNGSFEPAIVPKK
                     TRRLNNINSVVLSLYSRGMTTRDIEAHLEEVYGANVSRELISNVTEVVVDEIKAWQSR
                     PLDEVYPILYIDGLRLRIKDNGVVTTKVAYLAIGVDLEGRKHALGVWIQDSEGAKFWQ
                     KVVIDLRNRGVRDILIACCDGLTGLPDAIRSIYPDTVVQTCVVHVIRNAMRFVSYKDR
                     KKVATSMRAIYTAATVDAAELALKDFDAQYGRQYPGAVDVWRNAWPEFVPFLDYPVEL
                     RKIVYTTNAIESINFQLRKITKNRGHFPDRDAAMKLLYLGLRNISSQRGGFSGTGTYN
                     WTVALNTLARLFPGRIPLC"
     gene            complement(1562..2168)
                     /locus_tag="VHE33_20885"
                     /pseudo
     CDS             complement(1562..2168)
                     /locus_tag="VHE33_20885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948855.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2590..>3158
                     /locus_tag="VHE33_20890"
     CDS             2590..>3158
                     /locus_tag="VHE33_20890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99969.1"
                     /translation="MSVWDDDDFDQHPVWGKVDQLASLLDDERLPKEVDAPQRLSIVR
                     LKTALELLREHRDRGVKAHYAKAMLDNVDNHLANAVLSPLTTFVSNPDNYGNYVNDAA
                     DHAEAIFAYVAQWPSLPAGGQASAAGRAFAEYKREAELALQTLGEQNAELTSQLETLR
                     AQMNTVEAKMAGVQDNYAVSLAERENEYL"
BASE COUNT          644 a         1021 c          906 g          587 t
ORIGIN      
        1 tcagtgtggg ctggttggag tacgcccaga cccgcgggtt tgtcaccgac ccggcgcggg
       61 tggcccaccc tcaggacaag ccacgggtgg aacggatggt gcagtatgtg cgcggcaact
      121 tcttcgccgg cgaagacttc ggagcctgtc acgaattttg tgtaagtcag aggtgatttg
      181 actacgagtt gtattctaac acaacggaat tcggcccggg aagagtctgg cgagtgtgtt
      241 caacgccaca gtccagttgt acgttccggt gcctgagaag cctcctcttt ggctggagat
      301 gttgcgcaac ccgaggtaca gcaacttcat cgcggcgtca cggtcgggga aatggccgcg
      361 gtttttggtg atcttgcgca gctggaagtt gatcgactcg atcgcattgg tggtgtaaac
      421 gatcttgcgt aattcgaccg gatagtccag aaacggcacg aattcgggcc aggcgttgcg
      481 ccagacgtcg acggcgcccg gatactgccg gccgtattgg gcgtcgaaat ccttgagcgc
      541 cagctcggcg gcatccacgg tcgcggcggt gtagatcgcg cgcatcgacg tggcgacctt
      601 cttgcggtcc ttgtaggaca cgaaccgcat ggcgttgcgg atcacgtgca ccacgcaggt
      661 ctgcaccacg gtgtcggggt agatcgagcg gatggcatcg ggcagcccgg tcagcccgtc
      721 gcagcaggcg atcaagatgt cgcgcactcc gcggttgcgc aggtcgatga ccactttctg
      781 ccagaacttc gccccctcgc tgtcttggat ccagactccc agggcgtgtt tgcggccctc
      841 caggtccacc ccaatcgcca agtaagccac cttggtggtg accaccccgt tgtccttgat
      901 gcgcagccgc aggccgtcga tgtagaggat cggatagacc tcatcgagcg ggcgggactg
      961 ccaggccttg atctcatcga ccaccacctc ggtgacgttg gagatcaatt cccgcgacac
     1021 gttcgccccg tagacctcct cgaggtgggc ttcgatgtcg cgggtggtca ttccccgcga
     1081 atacagcgac agcacaacgg aattgatgtt attgagtcga cgggtcttct tcggcacgat
     1141 cgccggctcg aacgatccgt tgcgatcgcg cggcacattg atttcgactg ggccgttgac
     1201 cgtgttcacc gtcttcgacg tggagccgtt gcgtgaatta ccgctgccgc gcccagctgg
     1261 gtctccagcc tcatacccca gatggtgggt catctcggtc tgcagcgagc gctccaacac
     1321 cgccttggtc aattcgttga gcaaaccctc cgcgccgtcg atcggcgtac cggatttcac
     1381 cgcatccttg atcagcgaat ccaacgtctc cgccgagaac gcctcggcca gccgccgcgc
     1441 agccgacttc tcctcagccg gcaaccgcgt ggtcttcgtc acaaaacact ccttctgtcc
     1501 gcccgttcgg cggtccgatg atggaccacc ccggacttac acagatggaa tgacacgccc
     1561 tcaggatctg ctcgggagct ccatagctgg ccaacgccgc gcgcaaccca tcacagactg
     1621 cccgagtgcg ctcacgagcc atcaaccgcg cgcagacaca catccgagag tgatcgtcga
     1681 tgccggtcaa ggctttggcg ctcgtgccat cagccagcgg aaaacccccc acgacgtcca
     1741 tctgccacag ctccatcgcc gcgccgcgct cccagcgttt ccatttgcgc aagcgccgat
     1801 cccgcagact cggatcgatc acgcccgctc gcaccagggc ccgataggcc gccgattccg
     1861 acggcagcgg ccgcccgacg cttggccagc tcaaacacca gccgccgcgg accccaatac
     1921 ggccgcgacc gacgcaattc caacagcgcc gcttccaccg cggccggcat ttgatgcgga
     1981 catgacaccg gccgatgcga ccgatcccta agcccttcta gaccctcggc ctcatagtgg
     2041 gccaaccagg catgcaacgt ctgacgcgac acgcccacct tctcagcaac cagcgacacc
     2101 gacaacccat cgctgatcac cgccaacaca gcctgatacc tctgctcggc cacggttaac
     2161 tccctcattc gaaggagtgt caagcatcaa ccgaagtaac tgtcaaccat cagccgaaac
     2221 accgtcaggc atcacccgaa aacaaaacgt cacgcatcaa ccgaggtcac acaaatactt
     2281 ggtggggcgg gcggggctcg aacccgcgac caacggatta tgagtccctt ggtctggcgc
     2341 tttggcaaat tctgctggat ctgaaaatcg cccggcagcg actccaatcc cctagttttc
     2401 cagcgcttct atttgaaaac ggggccaagc agcggctgct agctcaccac ggcaaatcag
     2461 taggttgggc tgcaaatgtg gacgcggtat ggacgcgggt atgcacaggc gaggtcgctg
     2521 gccttaacac gggcgacatg cccgctcgaa gcgtcggttt atgtcagacc ccctcgagag
     2581 cattcgatcg tgagcgtttg ggacgacgac gactttgacc agcacccggt ttggggcaaa
     2641 gtcgatcagc tcgcgagcct tttggacgac gaacgtcttc cgaaggaagt tgatgcaccg
     2701 cagcggctgt cgattgttcg actcaaaaca gcccttgagc tgctacgcga gcaccgagac
     2761 cggggtgtaa aggcccacta cgccaaggcg atgctcgaca atgtagacaa ccacctggcc
     2821 aacgccgtgc tctcaccgct gaccacgttt gtgtcgaatc cagacaacta cggcaattat
     2881 gtcaacgacg ccgctgacca tgccgaggca attttcgcct atgtggcgca gtggccgtca
     2941 ttgccagccg gcggccaagc tagcgctgca ggccgtgcct tcgccgagta caagcgagag
     3001 gccgaacttg ccctgcaaac cttgggggag cagaacgccg agctcacgtc ccagcttgaa
     3061 acactccgag ctcagatgaa cactgtcgaa gcaaagatgg cgggcgtgca ggacaactac
     3121 gccgtgtcgc ttgcggagcg cgaaaacgag tatctgga
//
LOCUS       DASZYN010000836         6875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_831571, whole genome shotgun sequence.
ACCESSION   DASZYN010000836 DASZYN010000000
VERSION     DASZYN010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831571"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1658)
                     /gene="crtI"
                     /locus_tag="VHE33_20895"
     CDS             complement(144..1658)
                     /gene="crtI"
                     /locus_tag="VHE33_20895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HVT99970.1"
                     /translation="MTVANRKHVAVIGAGLGGMSAAIMLARSGFQVTIFEKNPQVGGK
                     LNLLETRGFSFDLGPSIFTLPQIFRPLFDGDGRRLEDYITLQRVDPQWRNFFEDGTVV
                     DLWENTERMRDELARFGPQAFEEYRQFVNYSRRQFAIVDRGYLRQGLDTFRQFVRFYG
                     WSEARDLDYLRSMSGSIYRRLSNPYLRAIFEYFIKYVGSSALDAPGFMNLMPNIQLEF
                     GLWYVSGGLYQLARAFRRRLEETGVSLCLDHEVVRVEQKGQRVTGLTVRRRQDDVRTI
                     AADFVVSNMEVIPAMQRLLKSPSSVMKKLRRYQPSCSGIVLHLGLNRVYPQLAHHNFF
                     YSRDPHAHFRRVFREQRLPDDPTIYLVAPTRSDPAQAPAGCDNIKILPHIPSLNERYS
                     YSREDYLALKELCLDKLERMGLTDLRRHIVVEDFWTPYDIEARYASNRGSIYGVVCDR
                     RRNFAFKAPKQSSHFRNLFFVGGSVNPGAGMPMVTLSGQHVARMIQEQVSSGAP"
     gene            complement(1655..3517)
                     /locus_tag="VHE33_20900"
     CDS             complement(1655..3517)
                     /locus_tag="VHE33_20900"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HVT99971.1"
                     /translation="MSNVVVDVFSRARAAQTAWASLPVSRRCRVIAALRRELARRSDA
                     IARVIAQETGKPILDALSGDVLVTLEHLRYCQLQAARALRPRRVRRSSLFYAGTQFEE
                     YREPHGVVLVFGPANYPLQLSLVPAATALVAGNAVILKCSERAPATAALIDALCVTAG
                     VPRDLIQVLDDGPEVSAELMDAGPDFVFFTGSSRNGQQVAERAARHMIPGVFELGGKD
                     PALVFADCPLERTVEGVTYGAFSNAGRVCVGIRRVYIESTIYREFVTRLVDRVAQLRV
                     GDSAEVDLCPLPAGSHPMLCAHIQDALCRGASMLWPPDGEVAGETPTLLADVPVDARL
                     LTEEFFGPVLCVAPFHSETEAVALANASAFALGSSIWTGDRSRARRVAAQLSAGSCTI
                     NDVIRNAGNPWAAFGGNRSSGNGRYRGDEGFRAFTRVKSVMFAGHRRRREMHWFPFRE
                     RTARRLGTVMRIRHGAAGFLGRLMLLLPLLFGALLPASPAAQVQPETHLAIQVQLTSH
                     AHGELGYLIFAAPPGFPGDRSKAVRHGFLPIPNGGGRVTIQADLPPGTYAVSVYEDLD
                     GNHRLDHNFFGIPREPVGVSGNPPTRFGKPRFDECAFHLSGQAQTIDIRVVPGI"
     gene            complement(3517..5022)
                     /gene="crtI"
                     /locus_tag="VHE33_20905"
     CDS             complement(3517..5022)
                     /gene="crtI"
                     /locus_tag="VHE33_20905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HVT99972.1"
                     /translation="MIIGAGPGGLAAAMLLAQRGFRVQVFEKQEVIGGRNAEVRLGDY
                     RFDLGPTFLMMKFLLEELFAEGGRRSSDYLQLQALDPMYALHFPDKVLLARSAPSAMK
                     AEIEKHFPGEGASLERFLERESARFKKLYPCLQKPYGSLASLISPTLLAAVPHIAAGR
                     SLHSVLADYFRSEELRLAFTFQSKYLGMSPWDCPGLFTMIPYTEHAHGVYHVMGGLCR
                     IGDALAQVAREEEAEIHTSSPVERVLLDGRRACGVELASGEKVYCDDVVINADFGHAM
                     SMFFDRKALGRYKPSALRRRRFSCSTFMMYLGLDRTFDTEHHSIVFARDYRENIEAIT
                     HGRAAFQDVSLYIRNAGRTDPSGAPAGHSALYILVPVANNTSGIPWEEHKEILRESVF
                     RTLRERTPFGDVRPHIRREMVITPEDWEKKHSVFLGATFNMAHSWNQMLYLRPHNEFE
                     QFSNCYLVGGGTHPGSGLPTIFESARISANLICEKYQMAYPVAKPLEVALA"
     gene            complement(5196..5888)
                     /locus_tag="VHE33_20910"
     CDS             complement(5196..5888)
                     /locus_tag="VHE33_20910"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HVT99973.1"
                     /translation="MSRHAQGFAANVVDRTGRAVLDMKADKSGHKNTDPKVVDESLRP
                     YAIPEKLRMLRLRRSMGLAQLAEHTGFSAAMLSRLENGRLVPTLPTLTRIALVFNVGL
                     DYFFTDPRKRHVVAVSRKKERRHFPSDPKSDSIPWHFETLDFRANERRLNGFLAHFHP
                     LPAEKTKPHFHRGVELLYLIEGELEITIGVDTFRLDPGDAIYFDAMQKHAYRSLVKGS
                     CTAVVVTTGDGK"
     gene            6129..>6875
                     /locus_tag="VHE33_20915"
     CDS             6129..>6875
                     /locus_tag="VHE33_20915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99974.1"
                     /translation="MRPYAFPLLVLLATHFSSGALAATPRKLILGNTSTGETVAFNRA
                     ANGWGISVEGRGAPMLSQAESARLEISDAGSGDVRELNAAYEQVSRIPNGVAASAVIH
                     GGTSVSFLVRDTWKLEENVLSVQRTVTVQGTDAGGFSSSIVFSAPTLGWDDASYFAPG
                     VLYADPTYDGERSPGGTALYAAHHLSFREDILPSPMLGLYFRSGASVTMLDPTPRGDT
                     TEAESQLTDTVMTDARFQFGSLSVMQQQAVP"
BASE COUNT         1426 a         2115 c         2062 g         1272 t
ORIGIN      
        1 cggcaggttg cgttcctcat tgcgcgcggg gatgacgaca gaaagcgatg gcgcggcgac
       61 cggcagcgcc gggggacagg tgcgcatccg gcacatcagc agggagccgg ccgcgattcc
      121 gaagacacag agaaagggcg ctatcatggc gcaccggagg aaacctgctc ctgaatcatc
      181 cgcgccacgt gctgcccact gagcgtgacc atgggcatgc cggcgccggg attcacgctc
      241 ccccctacaa aaaagagatt gcggaaatga gagctttgct tcggcgcttt gaaagcgaag
      301 ttcctccttc gatcgcagac cacgccatag atcgagccgc ggtttgaagc atagcgggct
      361 tcgatgtcgt acggcgtcca gaagtcttca accacgatgt gccggcgcag atcggtgagg
      421 cccatgcgct ccagcttgtc gagacacagc tctttcagag ccagatagtc ctcgcgggaa
      481 taggaatagc gttcgttgag cgacgggatg tgcggaagga tcttgatgtt gtcgcatccg
      541 gccggcgcct gcgcgggatc gctcctggtc ggcgctacca gatagatggt ggggtcatcg
      601 gggagccgct gctcgcggaa tacccgccgg aaatgcgcat ggggatcgcg cgaataaaag
      661 aagttgtgat gggcaagttg cggataaacg cggttcagac caagatgcag aacgattccc
      721 gaacaggaag gctggtaccg gcgcagcttt ttcatgacag aggacgggct tttgagcagc
      781 cgctgcatgg ccgggatgac ttccatattg gagaccacga agtcggctgc gatcgtccgg
      841 acatcgtctt gtctcctccg taccgttaac cccgtaaccc tttgaccctt ctgttccaca
      901 cgaaccacct cgtgatccag acagagcgaa accccggttt cctccagtct tctccgaaaa
      961 gcgcgcgcca actgatacag gcctccggag acataccaga ggccgaactc cagctggata
     1021 tttggcatca ggttcatgaa gccaggcgcg tccagggcgc tggaccccac atacttaatg
     1081 aaatattcga agatagcgcg caggtaagga ttgctgaggc gccggtagat gcttcccgac
     1141 atcgagcgga gatagtcgag atcgcgagcc tcgctccagc catagaaacg aacgaactgg
     1201 cggaaggtat ctagtccctg gcgaagatac ccgcgatcaa caatggcgaa ctggcggcgg
     1261 ctgtagttga cgaactgccg gtattcctcg aaggcctgtg ggccaaagcg cgcgagttcg
     1321 tcgcgcatcc tctccgtgtt ttcccacaga tcgacgacgg tcccgtcttc aaagaagttc
     1381 cgccactgcg ggtcgacgcg ctgcaacgta atgtagtcct caaggcgccg gccatcgccg
     1441 tcgaaaagcg ggcgaaagat ttgggggagg gtaaagatcg agggcccgag gtcgaagctg
     1501 aatcctcttg tttccaggag gttgagcttg cccccaacct gaggattctt ctcgaagatc
     1561 gtgacctgga atccgctgcg cgccagcatg atggctgcgg acatgccgcc caatccggcg
     1621 ccgataacag caacgtgctt cctgttagcg accgtcatat cccgggcacc accctgatgt
     1681 cgatggtttg tgcctggccg ctcaggtgaa aggcgcactc gtcaaagcgc ggctttccaa
     1741 aacgcgtcgg cggattgcct gagaccccaa ccggttccct cggaatgcca aagaaattat
     1801 ggtcaaggcg gtggttgcca tcgaggtctt catacacgct gaccgcatac gtgccgggcg
     1861 gcagatcggc ctggatcgtc acccgccctc cgccgtttgg aatcggcaga aagccgtgcc
     1921 gcaccgcttt agagcggtcc ccagggaatc ctgggggcgc agcaaaaata aggtatccga
     1981 gctcgccgtg ggcgtggctg gtgagctgga cctgaatggc gagatgcgtc tccggctgga
     2041 cctgcgccgc agggcttgca ggcagaagag cgccgaacag aagggggagg agcaacatca
     2101 ggcggcccag gaaacctgca gcgccgtgac gaatccgcat caccgtgccg agccggcgcg
     2161 ccgtgcgctc gcggaaggga aaccagtgca tctctcgcct ccggcgatgt ccagcaaaca
     2221 tcacggactt cacgcgagta aacgcacgga aaccttcgtc gccgcgataa cggccattgc
     2281 cgctgctgcg attgccccca aaagccgccc atgggttccc tgcattccgg attacatcgt
     2341 tgatggtgca gctgcccgcg gaaagctgtg ctgctacgcg tcgggctcga gatcgatcac
     2401 cggtccaaat gctgctgcca agagcgaagg cgcttgcatt ggccagggcc acggcttctg
     2461 tttcgctgtg gaacggagca acgcagagaa cgggaccaaa gaactcctca gtgagcagcc
     2521 gcgcatcgac gggaacatcc gcgagcagag tcggcgtttc accggcgacc tcgccatccg
     2581 gtggccaaag catcgaggca ccgcggcaca acgcgtcctg gatgtgcgcg cacagcatcg
     2641 gatgtgatcc ggccggtaaa ggacagagat caacttcagc gctgtcgccc acacgaagct
     2701 gagcgacgcg atccacaaga cgcgttacga actccctgta aatcgtgctc tcgatataaa
     2761 cgcggcgaat ccccacacac acccgtcccg cattgctgaa cgcaccgtag gtcacgcctt
     2821 ccaccgtccg ctccaaagga cagtcagcga agaccagcgc cgggtccttt cctcccagct
     2881 cgaatacccc gggaatcatg tggcgcgccg cgcgctcggc aacctgctgt ccgttacggc
     2941 tgctgcctgt aaaaaacacg aagtcggggc cggcatccat tagttccgcc gacacctccg
     3001 gaccatcgtc caggacctga atcaaatcgc gcggaacgcc ggccgtcacg catagcgcgt
     3061 cgatcagcgc cgccgtcgcc ggcgcgcgct cagaacactt gagaatgacc gcattgccgg
     3121 caacaagagc cgttgccgcc ggcacgagcg aaagctggag aggatagttg gccggcccaa
     3181 aaaccagcac gactccatgc ggttcccggt attcctcgaa ttgcgttccg gcatagaaaa
     3241 gagaggagcg ccggaccctg cgcggcctca gcgcccgggc ggcctgcaac tggcaataac
     3301 gcagatgctc cagcgtgacc agaacatccc ccgaaagggc gtcaagaatc ggcttgcctg
     3361 tttcctgcgc aatcaccctg gcaatggcat cggatctccg cgcaagttcg cgccgcagag
     3421 ctgcaatgac acggcaccgg cgcgacacag ggagtgaagc ccaggccgtc tgcgccgctc
     3481 gcgcgcgcga aaatacgtcc acgactacgt tggacatcac gcgagcgcca cctcgagagg
     3541 cttcgcgacc ggataggcca tttggtactt ttcgcagatt aagttggcgg aaatccgtgc
     3601 cgactcgaag atggtcggca aaccgctgcc tggatgggta ccgccgccga ccagatagca
     3661 gttcgagaac tgctcaaact cattgtgcgg gcgcaaataa agcatctgat tccagctatg
     3721 cgccatgttg aatgtggccc ccagaaagac cgagtgcttc ttctcccagt cttccggagt
     3781 gatgaccatc tcgcgccgga tgtgcggacg cacatcgccg aacggcgtgc gctcgcgcag
     3841 ggtgcgaaat acggactccc gcagaatctc cttgtgctcc tcccacggaa tcccgctggt
     3901 attgttggcg acgggaacga gaatatatag agcggagtgg ccggccggcg cgccggacgg
     3961 atcggtccgg cccgcgttgc ggatgtacag ggatacgtcc tggaacgccg cgcggccgtg
     4021 ggtgatcgcc tcgatattct cgcgatagtc gcgtgcaaag acgatcgagt ggtgttccgt
     4081 atcgaaggtc cggtcgagcc cgaggtacat catgaacgta gagcaggaga atcttcgcct
     4141 gcgcagtgcg gatggcttgt agcggccgag cgctttgcgg tcgaagaaca tgctcatcgc
     4201 atggccgaag tcggcgttga tgaccacgtc gtcgcagtac accttctcgc cgctcgccag
     4261 ttccacgccg cacgcccggc gcccatcgag gagaacgcgc tccaccggcg aagaagtatg
     4321 aatctccgct tcctcttctc gcgcaacctg cgcgagagca tctccgatgc ggcacaggcc
     4381 gcccatgacg tggtaaacgc cgtgggcatg ctcggtatac ggaatcatgg tgaaaaggcc
     4441 cggacagtcc cacggcgaca tgcccagata cttggactgg aaggtgaatg ccagccggag
     4501 ctcttcagat cggaaatagt cggcaaggac gctgtgcaga gagcgtcccg cggcgatatg
     4561 gggcacggcc gcgagcagtg tgggactgat caggctggcc agcgaaccgt acggcttctg
     4621 cagacagggg tagagctttt tgaagcgagc gctttcgcgc tcaaggaagc gctcgaggct
     4681 cgcgccttct ccgggaaagt gcttttcgat ctccgccttc atggctgagg gggctgagcg
     4741 cgccagcaga accttgtcgg gaaagtggag ggcgtacatc gggtcaagcg cctgaagctg
     4801 cagataatcg ctcgagcggc ggcctccctc ggcaaacagt tcttcgagca ggaacttcat
     4861 catcagaaac gttggaccca gatcgaagcg gtaatcgccg agccgcacct ccgcattgcg
     4921 tccgccgatg acctcctgct tttcgaatac ctgtacccga aatcctcgct gcgcaagaag
     4981 catggcggcc gcgagccccc cggggccggc accgatgatc acgatgtgtc tttttggacc
     5041 acgccgatag acgcgcatag gtcgaggcta atgacggcgc gcgcctgatt gacgcaacga
     5101 tgctgagggc atcgcgatta cttgatccag gcgcaacaaa ccgccgattt gttgcagttc
     5161 agttaagatg gccggtccgc atctcgctga atcgtctatt tgccgtcgcc ggtcgtgacc
     5221 acgaccgcgg tgcaggatcc ctttaccagg ctccgatagg catgtttttg catggcgtcg
     5281 aagtagattg catctccggg atcgagccgg aacgtatcga caccaatcgt gatctccagc
     5341 tcgccctcaa tgagatacag cagttcaaca cctcgatgga aatgcggctt ggtcttctcc
     5401 gccggcagag ggtgaaaatg cgccaggaat ccgttgagcc tgcgctcgtt ggcgcggaag
     5461 tccagcgttt caaaatgcca tggaatggaa tccgatttcg gatcggaggg gaaatgcctg
     5521 cgctccttct tccgggagac ggcgacgacg tggcgcttcc gaggatcggt gaagaagtaa
     5581 tccagcccca cattgaagac cagcgcaatc cgggtgagcg tcggcagggt cggcaccagc
     5641 cgtccgttct ccagtcgcga gagcatcgcc gcggaaaatc ccgtgtgttc ggcgagctgc
     5701 gccaggccca tgcttcggcg caggcgaagc atgcgcagct tttccgggat cgcgtaaggc
     5761 cgcagcgatt cgtcaaccac ttttggatct gtgttcttat gacccgactt atctgccttc
     5821 atgtccagta ccgcccggcc ggtccggtcg acgacattcg cagcaaatcc ctgcgcatgc
     5881 cgagacaccg tgaatcgttc gggatcccga atccaattct ctgaccgtcg actccttcgc
     5941 ggcttcgggc ccggattcaa cgccgcctct aaactgggat tgtagcggga acgtacgccg
     6001 tcctcccttc actcgccacg gcaatgcacc cgaatctccc tcactggcgc gccgtgatgt
     6061 cgtgatttgg tcagagcctg cgcgagtgat cttctcgtca tggagaaaag agtgcaaact
     6121 ggatcagcat gcggccttac gcgtttccgc ttctcgtcct gctcgcaact cacttcagct
     6181 ccggcgctct tgcggccaca ccccgaaagc tcattctcgg gaatacctcg accggtgaaa
     6241 cagtcgcgtt caatcgcgcc gcgaatggct ggggcatcag cgttgaaggc cggggtgcgc
     6301 ccatgctgtc gcaggccgag tccgcgcgcc tcgagatctc tgacgccggt tccggagacg
     6361 tacgagaact gaatgcggct tacgagcagg tctcgcgcat accgaacggt gtcgcggcca
     6421 gtgccgttat tcacggcggc acatctgttt ccttccttgt ccgagacacc tggaagctgg
     6481 aggaaaatgt cctctccgtt caacgcaccg tgacggttca ggggaccgat gcgggcggct
     6541 tctcctcttc gatcgtcttc tctgctccca cattgggttg ggatgacgcc agctattttg
     6601 ctcccggcgt cctgtatgcc gaccccacgt atgacggcga acgctcaccg ggcggcacgg
     6661 cactctatgc tgcccatcac ctcagctttc gcgaagacat tctcccctcg ccgatgttgg
     6721 ggctgtattt ccgcagtggc gcatccgtaa cgatgctcga cccaacgcca cgtggcgaca
     6781 ccacagaggc cgagagccaa ttaaccgaca cggtgatgac cgacgcccgc ttccagttcg
     6841 gatcactcag cgtcatgcaa cagcaggcag tcccg
//
LOCUS       DASZYN010000837         2818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_320453, whole genome shotgun sequence.
ACCESSION   DASZYN010000837 DASZYN010000000
VERSION     DASZYN010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320453"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1661)
                     /gene="cysS"
                     /locus_tag="VHE33_20920"
     CDS             complement(120..1661)
                     /gene="cysS"
                     /locus_tag="VHE33_20920"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HVT99975.1"
                     /translation="MSVTKQSGEETGVVSVDAPVKMRLHNTMSGRIEELAPSDGKALR
                     MYACGPTVYDYGHIGNFRTFLQVDVLRRSLRLLGTDVRHVMNITDVDDKIIRNATAAG
                     VEIDTYTKKFQEAFFEDLDALRVERPEVIARATEHIPSMVKLIEQLAAEDIAYRAEDG
                     SWYFRIARFPAYGKLSKKDFSGIADGARIDVDEYEKDSARDFALWKAPKPGEHKWETP
                     LGPGRPGWHIECSAMAMEYLGESFDLHGGGEDLMFPHHENEIAQSESATHRTFARHWF
                     HVRFLLVEGRKMSKSEGNFYTLRDLLLRGYKASAIRMLLISVPYRQQLNFTFEGLAAE
                     TIAVERLRTFRDRIANTKWPASDVAGNEVAALARETRAKFRAALADDLNTAEARAAIF
                     EMVRAVNARADAGKFFASDQASVLDVLREFDQVFAVLEDHDAEWTKFTLDWAEREGKL
                     DKAAPELLATRGVTDEKIQALVEERDQAKRRRDFKRADAIRNELSEMGVLLEDSKEGT
                     RWKRK"
     gene            complement(1771..>2818)
                     /gene="tsaD"
                     /locus_tag="VHE33_20925"
     CDS             complement(1771..>2818)
                     /gene="tsaD"
                     /locus_tag="VHE33_20925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HVT99976.1"
                     /translation="PELASREHLRNILPVVRESLKTAGVGYGDIDAIAVTEGPGLAGA
                     LLVGISFAKSLAAALGKPLIAVNHLEGHIHAVLLEEARRTAESDGGAPEEMRSGRPVL
                     ALVVSGGHTHLYLAEFSADARAWTYRNVGRTVDDAAGEAFDKVAKLLGLGYPGGPWID
                     ALAAHGNPEAVPFTFSQIKAKAHRKGEPGALDPAEEIYFSFSGIKTAMLRYTKVYEMG
                     DSIEARRSALARMENATPADVLALCDRRMLDLIASFQRSVVDDLRRKTFRAVERFGAE
                     RVIVSGGVAANRELRVRFTAEAAERHLPIAFPSMALSTDNAAMIAAAAWPKLLAGEFA
                     AEDLTAEPALALGR"
BASE COUNT          507 a         1045 c          723 g          543 t
ORIGIN      
        1 cctgcgagcc gttcaggccg cgatgccccg cgtcaaaccg ctggtcgccg ccttcgagag
       61 aatcgctgaa actgaacccg tggccggttt gcccttcgag ccgtgagact gcactcgcct
      121 tacttccgct tccagcgcgt cccctctttc gagtcctcca gcaaaactcc catctccgat
      181 agctcgttcc ggatcgcatc cgcccgcttg aagtctcgcc gccgcttcgc ctggtcgcgc
      241 tcttcgacca gcgcctgaat cttctcgtcc gtcacgcccc gtgttgccag cagctccggt
      301 gccgctttgt ccagcttgcc ttcgcgctcc gcccagtcca gggtgaactt tgtccactcc
      361 gcgtcgtgat cctcgaggac cgcaaacacc tggtcgaact cccgcagcac gtccagcaca
      421 gacgcctgat cgctcgcgaa gaatttcccc gcatccgcac gcgcgttgac cgcccgcacc
      481 atctcgaaga tcgctgcccg cgcctctgcc gtgttcagat catcggccag ggccgcgcgg
      541 aacttcgccc gcgtctctcg cgccagggcc gcaacctcgt tgcccgcaac atccgaagca
      601 ggccacttcg tattcgcaat gcgatcccgg aaggtccgca ggcgctccac agcgatggtc
      661 tccgctgcca gcccttcaaa cgtaaagttc agctgctggc gatagggaac cgagatcagc
      721 agcatccgga tcgccgaagc cttatacccg cgcagcagca ggtcgcgcag cgtatagaag
      781 ttgccctccg acttcgacat cttccggcct tccaccagca ggaagcgaac atggaaccag
      841 tgccgcgcga acgtgcgatg cgtcgccgac tccgactgcg caatttcatt ctcatggtgc
      901 ggaaacatca gatcctcgcc gccgccatgc aggtcgaagc tctctcccag atactccatc
      961 gccatcgccg agcactcgat gtgccaaccg ggccgccccg gcccgagcgg cgtttcccac
     1021 ttgtgctcgc ctggcttcgg cgctttccac aacgcaaaat cccgcgccga atccttctcg
     1081 tattcatcca catcgatgcg cgcgccgtcc gcgattccgg aaaagtcctt cttcgagagc
     1141 tttccgtacg ccggaaaccg cgcgatccgg aaataccacg aaccatcttc ggcgcgatag
     1201 gcaatgtcct ctgccgccaa ctgctcgatc agtttcacca tcgacggaat atgctccgtc
     1261 gcgcgcgcaa tcacctctgg ccgctcgacc cgcagcgcat ccagatcctc gaaaaaagcc
     1321 tcctgaaact tcttcgtata ggtgtcgatc tcgacgccgg ccgcggtcgc gttgcgaata
     1381 atcttgtcgt ccacatcggt gatgttcatg acgtggcgga cgtcggtccc cagcagcctg
     1441 agcgaccgcc ggagcacatc cacctgcagg aatgtgcgga agttcccgat gtgcccgtag
     1501 tcatagacgg tcggtccaca cgcatacatc cgcagcgctt tgccgtcgga gggagccagc
     1561 tcctcgatcc gccccgacat cgtgttgtgc agccgcatct tcaccggcgc atctaccgac
     1621 acgactcctg tttcctcgcc actttgcttc gtcacactca tggagaccaa ttcggattct
     1681 accagcgcat ggaagctcgc ccgggaccgt tccgggcgct tcgaatcctg tccgcctgta
     1741 cgctgtgccc tgagccctgc gctctgtttc tcaccgcccc agggccagtg ccggctctgc
     1801 tgtcaggtct tccgctgcga attctccggc cagcagtttc ggccaggcgg ccgccgcaat
     1861 catggccgcg ttatcggtcg aaagcgccat cgacggaaac gcgatcggca gatgccgctc
     1921 cgccgcctcc gccgtgaatc gcacgcgcaa ctcgcgattc gccgccacgc cccccgaaac
     1981 gatcacccgt tccgctccaa atcgttccac cgcgcgaaac gtcttccgcc gcagatcgtc
     2041 caccaccgag cgctggaacg acgcaatcag gtccagcatc cgccgatcgc acagagccag
     2101 cacatcggcc ggcgtcgcat tttccatccg cgccagtgct gaccgacgcg cctcaatcga
     2161 atcccccatc tcatacactt tcgtgtagcg cagcatcgcc gtcttgattc cgctgaagga
     2221 gaagtagatc tcctcggcag gatccagcgc gcctggctct cccttgcgat gcgctttggc
     2281 cttgatctgc gaaaacgtga acggcaccgc ctcgggattg ccgtgggcgg caagcgcgtc
     2341 gatccacggc ccgccgggat aacccagccc cagcagcttc gcgaccttgt cgaaggcctc
     2401 gcccgccgcg tcgtccactg tccggcccac attgcgatac gtccaggccc tcgcatctgc
     2461 ggaaaattcc gccagataca aatgcgtatg accgccactc accaccagag cgaggactgg
     2521 ccgtccagac cgcatctctt caggcgcacc gccatctgat tccgccgttc gccgtgcttc
     2581 ttccagcaaa actgcgtgga tgtgcccttc cagatgattt accgcgatca gcggcttgcc
     2641 cagcgccgcc gccagcgact tcgcgaagct gatgcctacc agcaatgcac cggcaagccc
     2701 gggtccttct gtaaccgcga tggcatcaat atccccatat ccaactccgg ccgttttcag
     2761 cgattcccgc accaccggca gaatgtttcg taaatgctcg cgcgaagcca gctccggc
//
LOCUS       DASZYN010000838         1865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_513568, whole genome shotgun sequence.
ACCESSION   DASZYN010000838 DASZYN010000000
VERSION     DASZYN010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513568"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VHE33_20930"
     CDS             <1..286
                     /locus_tag="VHE33_20930"
                     /inference="COORDINATES: protein motif:HMM:NF025197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HVT99977.1"
                     /translation="HIGSVVNAPDQDTADRDAVDVCGSKGAPGCATILRFVDGCGAVA
                     RRDDVHTVGTGATLQEAERNAIAALGPDRPPTLSSDGSGPAVISASRCNG"
     gene            906..>1865
                     /locus_tag="VHE33_20935"
     rRNA            906..>1865
                     /locus_tag="VHE33_20935"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          392 a          479 c          625 g          369 t
ORIGIN      
        1 gcacatcgga agtgtcgtca acgcgcccga tcaggacacg gccgaccggg atgcggtgga
       61 cgtgtgcggg tcgaaggggg ctcccggttg cgcgaccatc cttcgcttcg tcgacggatg
      121 cggggcggtc gccaggcgtg acgacgttca taccgtcgga accggtgcca ccctgcagga
      181 ggccgagcgc aacgccatcg cggcgctggg cccggatcgc ccgccgaccc tgagttcgga
      241 cggttcgggt cccgccgtca tcagcgcgtc gcgctgcaac ggctgacccg cagccgaaat
      301 cgtcgcgtat ccccgagacg cgtatcgcga atagcgatcg gaagccggtc ccgcccccac
      361 cgcgggccgg cttccggcat ttcggggcgg gcttcggccg acggtcggaa tttctcgccg
      421 gacgccgatg ttgtgaatgg tgttgcgagc gcatccgatg tgcgtcgtca ggccggatga
      481 ccaggcgatt tgacttcgaa tgcggttggc acgtaactta ttccaggtca gagcgacacg
      541 gacaccgacc cggagccgag aggcccggga caacgaggtc ggacgaggag cgcctgacaa
      601 tcacactggt tcgaccgaga cccggatttg gttcgggtcc gatggttgag ctaagctgga
      661 aaagttgcct cgccgatcga acagatgaaa atctgggaga tggtgagtgc gtgtgttctt
      721 tgagaactca atagtgtgtc gatgaatgtc agtgccaaat atttattttg gttccgttca
      781 tcctcccccc gtgagggtga cggacatttt tgtcagctaa ttttccggct ggcgtttaag
      841 ttaggttttc ggactctggt tcgagatact tctgattggc tgcttcggtg gcaaatctag
      901 agtcttcaac ggagagtttg atcctggctc aggacgaacg ctggcggcgt gcttaacaca
      961 tgcaagtcga gcggtaaggc ccttcggggt acacgagcgg cgaacgggtg agtaacacgt
     1021 gggtgatctg cctcgcactc tgggataagc ctgggaaact gggtctaata ccggatatga
     1081 ccacggatcg catggtttgt ggtggaaaga tttatcggtg cgagatgggc ccgcggccta
     1141 tcagcttgtt ggtggggtaa tggcctacca aggcgacgac gggtagccga cctgagaggg
     1201 tgaccggcca cactgggact gagacacggc ccagactcct acgggaggca gcagtgggga
     1261 atattgcaca atgggcgaaa gcctgatgca gcgacgccgc gtgagggatg acggccttcg
     1321 ggttgtaaac ctctttcgac agggacgaag cgcaagtgac ggtacctgta gaagaagcac
     1381 cggccaacta cgtgccagca gccgcggtaa tacgtagggt gcgagcgttg tccggaatta
     1441 ctgggcgtaa agagcttgta ggcggttcgt cgcgtcgatt gtgaaaactt gcagctcaac
     1501 tgcaagcttg cagtcgatac gggcggacta gagtacttca ggggagactg gaattcctgg
     1561 tgtagcggtg aaatgcgcag atatcaggag gaacaccggt ggcgaaggcg ggtctctggg
     1621 aagtaactga cgctgagaag cgaaagcgtg ggtagcgaac aggattagat accctggtag
     1681 tccacgccgt aaacggtggg tactaggtgt gggtttcctt ccacgggatc cgtgccgtag
     1741 ctaacgcatt aagtaccccg cctggggagt acggccgcaa ggctaaaact caaaggaatt
     1801 gacgggggcc cgcacaagcg gcggagcatg tggattaatt cgatgcaacg cgaagaacct
     1861 tacct
//
LOCUS       DASZYN010000839         4310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_438718, whole genome shotgun sequence.
ACCESSION   DASZYN010000839 DASZYN010000000
VERSION     DASZYN010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438718"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1577
                     /locus_tag="VHE33_20940"
     CDS             180..1577
                     /locus_tag="VHE33_20940"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HVT99978.1"
                     /translation="MTVASPMLQKFGPKIKTELPGPEARRIVEADGRLISPSYTRSYP
                     MVAKRGRGVRVEDVDGNEFLDFAAGIAVVSTGHCHPEVVAAIQQQAGELIHMSGTDFY
                     YESLITLSERLSSIAPMPGPHRFYYGNSGAEAIECALKIARYHTGRQNIIAFFGAFHG
                     RTMGALSLTASKPQQRRRFAPLVPGVTHIRYPYAYRGCSGGPQEEEAFALGCARYIEE
                     KLFHTTLAPEEVAAIFVEPIQGEGGYVVAPTVFMRELRRICDKYGILLVADEVQSGAG
                     RTGKWWAIEHTGVEPDIVTMAKGIASGMPLGVCMTRAAIMDWKPGSHASTFGGNPVCI
                     AAALKTLDILEREGIGNAAAQGEKMFARLRQWPSRHAVIGDVRGRGLMIGLEIVKDQE
                     TRESGPQLRDRIVDLAFERGLLILGCGENSIRLAPPLIVNEHEAAVALDILEECVTLA
                     GQEVAQPATVAGAAV"
     gene            complement(1630..1833)
                     /locus_tag="VHE33_20945"
     CDS             complement(1630..1833)
                     /locus_tag="VHE33_20945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99979.1"
                     /translation="MSRNFNWIWYLGAAVWFLNAALAMHHGKLRLGLANAAISAVFLA
                     AGIFFGRQAKRSSGRGNGRGPLK"
     gene            2284..>4310
                     /locus_tag="VHE33_20950"
     CDS             2284..>4310
                     /locus_tag="VHE33_20950"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HVT99980.1"
                     /translation="MKTYQGSEIRNVAVVGHAHSGKTTLVSALLHAAKMTPSLGRVED
                     GSAVTAYDEEDVARGTTMQNAVAFAEWNGVKINFIDTPGFHMFVHEARAAMAPVEAAL
                     VAVNAVTGVEPMTERVWKYAEEFNLPRAVVINQMDHPRAAASAESTLATLAERFGRQC
                     IPVQLPIADGKGFEGVVDLVTMEAFFYTPGGDGHGRIGEIPESLKEPAQRAHETLVEL
                     VAEGKDELMEEYFREGTIPEQHLITALHEAIREDRIFPVLYSSGLANVATDHLLDFLK
                     VYAPAPIEREPVAARAVLHTVAAAANGGGNGTAATAEGQSPEIVMRKVEDKEPLSLYV
                     FKTMTDPFSGRISFFKVFSGILKNDASAQNYSRRTAEKFSHLSIMQGRTAVAVGELHA
                     GDIGAVAKLKETLTGDTLGDKNSEIFYEPVTMPEPAMTWAIEPRSRADEDKLAPAVHK
                     LMEEDLMVRFFRDTQTNEFLIAAAGQSHIEALVSKLRKRYHTEVNLKAPRVPYRETIR
                     GRADAQGRHKKQTGGHGQYGDCKIKLEPLARGGGFEFENEVFGGAIPRNFIPAIEKGI
                     QESAARGYLAGYPVVDFKVIVYDGSYHDVDSNELSFKMAGRIAFRKCMEQAKPALLEP
                     IMSVEIEAPQEFAGALMGDLNQRRGRVQGMDSHGANSIIRAEVPMAEMLTYG"
BASE COUNT          851 a         1332 c         1331 g          796 t
ORIGIN      
        1 cctgcacgcg accgagtctc ttcggcagcg actagtcacc gctgccctca acgctggcgc
       61 accagcgtcg agtccgaaga tgacctctct cacccgcatc gccatcgttc tcccgccgtt
      121 tgccgatggc tcgaccaccg caggctcaat tgccgcaacc gctcgcatag gagagaccca
      181 tgactgtcgc aagcccaatg ctgcagaagt tcggcccaaa gatcaaaacg gagctgcccg
      241 gcccggaggc aaggcgcatc gttgaagccg acggccgcct catttcgccc agctacacgc
      301 gttcctatcc catggttgcg aagcgcggac gcggcgtgcg tgtggaagac gtagacggca
      361 atgagtttct ggattttgcc gcgggcatcg ccgtggtctc gaccggccac tgtcatccgg
      421 aagtggtagc agcgatccag cagcaggccg gcgagctgat ccacatgtcg ggcacggact
      481 tctactacga gagcctgatc acactatcgg agcggctttc gtcgatcgcg ccgatgcccg
      541 ggccacaccg gttttactac ggcaactccg gcgccgaggc tattgaatgc gcgctgaaga
      601 tcgcccgcta ccacaccggt cgccaaaaca tcatcgcttt cttcggcgcc tttcatggac
      661 ggacgatggg ggcgctctca ctgacggcat ccaagccgca gcaaaggcgg cgctttgcac
      721 cgctggttcc cggcgttacg cacatccgtt acccctacgc gtatcgcggc tgcagcggcg
      781 ggccgcagga ggaagaggct tttgcgctgg gctgcgcgcg gtacatcgaa gagaagctct
      841 ttcacaccac actggcaccg gaggaagttg cggcgatctt cgtcgagccc attcagggcg
      901 agggcgggta cgtcgtcgcg cccacagtct tcatgcggga gctgcgccgc atctgcgaca
      961 agtacggcat tctgctggtg gccgatgagg tacagtcagg cgccgggcgg acgggcaaat
     1021 ggtgggccat tgaacatacc ggtgtggagc ctgatatcgt caccatggcc aagggcattg
     1081 cctcgggcat gcctctggga gtttgcatga cgcgcgccgc gatcatggac tggaagccgg
     1141 ggtcccacgc ttcgacgttc ggcggaaatc cggtctgcat cgctgccgcc ctgaagactc
     1201 ttgacatcct ggagcgcgaa ggcatcggca atgcagctgc tcagggcgag aagatgtttg
     1261 ctcgcctccg gcaatggccg tcgcggcatg cggtgattgg cgatgttcgc gggcgcggtc
     1321 tgatgattgg cctcgagatc gttaaggatc aggaaacgcg tgagtcaggg ccgcagctgc
     1381 gcgatcggat tgtggacctg gcattcgagc gcgggctgtt gattcttggc tgcggtgaga
     1441 actcgattcg cctggcgccg ccgctcatcg tgaacgagca cgaggctgcc gtcgcgctcg
     1501 atattcttga ggagtgcgtg acgctggcgg gacaggaagt ggcgcaacct gctactgtcg
     1561 ccggcgcggc ggtctaactc cgcgtggaag ttcagggcga ggccggaatc cttttccagc
     1621 ctcgccttgc tatttcagcg gaccgcgacc gttgccgcgg ccggaggagc gtttcgcctg
     1681 tctcccgaag aagatgccgg ccgccaggaa aactgcggaa atagccgcgt tggcgaggcc
     1741 caggcgcagc ttgccgtggt gcatggccag cgctgcgttc aggaaccaaa cagcggcgcc
     1801 gagataccaa atccagttga aattgcggct cactctctta tgggatgcgc ctgcagtggc
     1861 gcatgccttt ccctgccatc catactatga cgcttacctt ggcagacacc gcgcctgggc
     1921 cgattgttcc atgcctgtca ggaaccctcg ccgccgacac cccacccctg cgtgtgctac
     1981 catccctgtt cctgtgaacg aggggtgaat cgctttccga aatcccctcc tcatgaatgt
     2041 tctttgtatg ccggacgagg atcgaagatc cgcccgttcc ggcacgcatt tgtcgcgcgc
     2101 cggagcaaat cccgtctccg atcagacgcc cctcaagacg ccttccggaa atccctgtgt
     2161 tcggcgagcc tgtatccgcc gtcacagttc ctccacagga attgatttct acttgcctgt
     2221 ctctgaacca cgccacaatc gcggccgcga gccgcagcaa ctttcttttt caaggagcac
     2281 tccatgaaaa cctaccaggg aagtgagatc cgtaacgtag ctgtagtggg gcatgcgcac
     2341 agcggcaaga ccacgctcgt ctccgctctg ctgcatgccg caaagatgac acccagcctc
     2401 ggccgtgttg aagatggatc ggccgttacc gcctacgacg aagaagacgt tgctcgtggc
     2461 accacgatgc agaacgccgt ggccttcgca gaatggaacg gcgtgaagat caactttatt
     2521 gatacacccg gctttcatat gttcgtgcac gaggcgcgcg ccgcgatggc gcccgtcgaa
     2581 gcagcgctgg tcgcggtgaa tgcggtcacc ggcgtcgagc ccatgaccga acgcgtctgg
     2641 aagtacgctg aggaattcaa cctgccgcgc gccgtagtca tcaatcagat ggaccacccc
     2701 cgcgccgcag cgtccgccga atcgacgctg gccacgctcg cagagcgctt cggccggcag
     2761 tgtattccgg tgcaactgcc gatcgccgac ggtaagggtt ttgaaggcgt tgtcgacctc
     2821 gtcaccatgg aggcgttttt ctatacgccc gggggggacg gtcacggcag gattggtgag
     2881 attcccgaat ctctgaaaga gcccgcgcag cgcgcgcacg agacgctggt ggagctggtg
     2941 gcggaaggaa aagacgagct gatggaagaa tacttccgcg agggcacgat cccggagcag
     3001 cacctgatca ccgctctgca tgaggcgatc cgagaggacc gcattttccc ggtgctgtac
     3061 tccagcggtt tggcaaatgt cgccacggat catcttctcg attttctgaa ggtctacgcg
     3121 cccgcgccaa tcgagcggga gccggtcgcc gcccgggcgg ttctgcacac ggtcgccgca
     3181 gcggcgaatg gaggtggcaa cggcaccgct gccaccgctg aagggcagtc tccggaaatc
     3241 gtcatgcgta aggtcgagga caaggagcca ctctcgctct atgtcttcaa gaccatgacc
     3301 gatccgtttt ccgggcgcat cagcttcttc aaggtgtttt cgggcatcct taagaacgac
     3361 gcaagcgcgc agaactactc gcgccgtaca gcggaaaaat tctcgcactt gtcgatcatg
     3421 caggggcgga cggctgtcgc cgtcggcgaa ctgcatgcgg gcgacatcgg cgccgtcgcg
     3481 aaactgaaag aaacactcac aggcgacacg ctgggggaca agaactcaga gattttctat
     3541 gagcccgtca caatgcccga gccggccatg acgtgggcca tcgagcccag gtcgcgtgct
     3601 gacgaggaca agcttgcgcc ggctgtgcat aagctcatgg aagaggacct gatggtccgc
     3661 ttcttccgcg acacgcagac gaacgaattc ctgatcgcgg ccgcagggca gtcgcacatc
     3721 gaggcgctgg tgagcaagct gcgcaagcgt tatcacaccg aggtcaacct aaaggcgcca
     3781 cgcgtgccct atcgcgaaac gattcgcggg cgcgcggacg cgcagggtcg acacaagaaa
     3841 caaaccggtg gacacggcca gtacggcgac tgcaagatca agctggagcc actggcccgc
     3901 ggcggcggct tcgaatttga aaatgaagta ttcggcggag ccatcccgcg caacttcatc
     3961 cctgctatcg aaaaagggat tcaggaatcc gccgcgcgcg gctacctggc cggttatccg
     4021 gtggtcgatt tcaaggtcat tgtctacgac ggcagctacc acgatgtcga ctccaacgag
     4081 ctgtcgttca agatggcggg ccggattgcc ttccgtaagt gcatggagca ggccaaacct
     4141 gctctgctgg aaccgatcat gagcgtcgaa atcgaggctc cgcaggaatt cgccggagcg
     4201 ctgatgggcg acctgaatca gcgccggggc cgcgtccagg gcatggacag tcacggcgct
     4261 aactccatca tccgcgccga agtgccgatg gcggagatgc tcacctacgg
//
LOCUS       DASZYN010000840         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_833317, whole genome shotgun sequence.
ACCESSION   DASZYN010000840 DASZYN010000000
VERSION     DASZYN010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833317"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VHE33_20955"
     CDS             <1..283
                     /locus_tag="VHE33_20955"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HVT99981.1"
                     /translation="TAFSFREANFAEVIVGVDPDPANNERMMRWAKDYWLALHPYSAG
                     GGYVNMMMDEGTDTVQAAYRDNYARLVQVKAAYDPGNLFHVNQNIKPGR"
     gene            686..1885
                     /locus_tag="VHE33_20960"
     CDS             686..1885
                     /locus_tag="VHE33_20960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HVT99982.1"
                     /translation="MSSESNTQHFSEAHYQLAMKAAGLGVWYWDLLQHQQVWSPECKA
                     LLGVPQEAEAKYELFLALVHPDDRGRIQTLLDESHHHKTPYSIEYRIIWPDGSLHWLA
                     DKGSYFYDRQGNATRLVGVIWDITMQKQVEETCAELERRKDEFIALASHELRTPLTSL
                     KGNLQLAQRFLRHFQDRSAVLVSERERTAFDHLVTWNERALRQANAECRLVNDLLEAN
                     ALRAGWLHVTQEPRDLVCLVRNAVDDVQILAQSHPLHLDLPEVATVPVMADEVRIGQV
                     ITNYIENALRHSQEQQPVTVGVTLGNETARVWVKDTGPGISLEEQRTLWERFRRVQSF
                     ASCTGGLGLGLYICQELIRLHGGQTGIASIPGQGATFWFTLPLCVERQPEASRGALPE
                     QLMKRAK"
     gene            complement(1956..>2414)
                     /locus_tag="VHE33_20965"
     CDS             complement(1956..>2414)
                     /locus_tag="VHE33_20965"
                     /inference="COORDINATES: protein motif:HMM:NF012762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose binding domain-containing protein"
                     /protein_id="HVT99983.1"
                     /translation="GTLNTTAYSVTIGGGNPPTPTPTRGSTPTATPTRQPTATSTPIS
                     QGGPCSVHYTVTNQWPGGFGANIIITNTGTTTISGWSLKFNFANGQTITQLWNGSYTQ
                     SGGSVTITNLSYNGSLAPGATANPGFNGSWNGTNSNPTSFTLNGAACSVV"
BASE COUNT          494 a          704 c          729 g          487 t
ORIGIN      
        1 aaccgccttc agcttccgcg aagccaactt tgccgaagtc atcgtcggtg tcgatcccga
       61 tcccgccaac aacgagcgca tgatgcgctg ggcaaaggac tactggctgg ctctgcaccc
      121 ctactcggcg ggcggaggct acgtcaatat gatgatggac gaaggcacag acaccgtgca
      181 ggcggcttac cgcgataact acgcccgcct ggtccaggtc aaggcggcct atgatcccgg
      241 caacctcttc catgtcaatc aaaacatcaa gccgggtcgc taagctgtgc cggtctgtgc
      301 taccggagat gtttcctgaa gcaattcgtc aaagagagtt gtggcgttcc agtggcccgc
      361 ccggcctgac gctctttggg gccgcgcagg tggaggactg ggcggccatt gacgccgtgc
      421 gtcagcgggc cttatcgcta catctcccct gtcgtccctg ctttctcagc taatgtccca
      481 tcggcactcc ctgcctgcgc ggcctgaccg tcgagcgggt tgtacaggag gctcaagaag
      541 tcctggccct ttcccggtaa cgcgggaggg tgtcagcagg cgatacagcg tgcttcttcg
      601 tgagtcgctt tcatgttagc cggcgtatta agaacgagaa ggcttctcca ggtctgcttg
      661 agattttttg tgaggtggtg ccaacatgag cagcgaaagc aacactcagc acttcagcga
      721 agctcactat cagcttgcga tgaaagcggc aggtctcggg gtatggtatt gggatctgct
      781 gcaacaccag caggtctgga gtccagaatg taaagctctc ctgggcgttc cccaggaagc
      841 ggaagcaaaa tatgaactgt ttcttgctct tgtccatccc gacgaccgtg gacgtattca
      901 gaccttactc gacgaaagtc atcatcataa aacgccatat agtatcgaat atcgcatcat
      961 ctggcccgat ggcagcctgc actggctggc cgacaaaggc agctactttt atgacaggca
     1021 gggcaatgcc accaggctgg tcggcgtgat ctgggatatc accatgcaaa aacaggtgga
     1081 ggagacgtgc gccgaattag agcgcaggaa ggacgagttt atcgctctgg cgagtcatga
     1141 actgcgcacc cctttaacct ctctgaaagg caatctgcaa ctggcgcagc gttttctccg
     1201 ccacttccag gacagaagcg ccgtgctggt ttcggaacgg gaacgcaccg cgtttgatca
     1261 tctggtcacc tggaacgagc gagcgttgcg ccaggccaac gcggaatgtc gcctggtcaa
     1321 cgatctgctg gaggccaacg ccctgcgggc aggctggctg catgtcacgc aggaaccccg
     1381 cgacctcgtc tgcctggtgc gtaacgcggt ggatgatgtg cagatccttg cccaatccca
     1441 cccgctgcac cttgatctgc cggaggttgc gacggtgccc gtcatggctg acgaagtgcg
     1501 catcggtcag gtcatcacga attacatcga aaacgcgctg cgccactccc aggaacagca
     1561 gccggtgaca gtaggcgtca ccctgggaaa cgagacagcg cgggtctggg tcaaagatac
     1621 cggtcccggc atctcattgg aggagcagcg cacgctctgg gagcgctttc gccgcgtgca
     1681 gagcttcgcc agttgtaccg gcggactggg cctgggcctc tacatttgcc aggaactcat
     1741 ccgcttgcat ggaggacaga ccggcatcgc cagcatcccc ggtcagggcg cgaccttctg
     1801 gttcacgctt cccctttgcg tggagcgcca gccggaagca tccagaggcg ctctcccgga
     1861 acaactgatg aagagggcaa aatagccagc gatctcgcat gtctgtgaga tcgctggccg
     1921 gctaccgatg aactggcgag aacccctgac cgggattaaa cgacgctaca ggccgcgccg
     1981 ttgagggtaa acgaggtcgg attggagtta gtgccattcc acgagccatt aaagcctgga
     2041 ttggccgtcg ctcccggcgc gagcgagccg ttataggaaa ggttggtgat ggtcaccgag
     2101 cctcccgact gcgtatagct gccgttccag agctgcgtga tggtttgccc gttggcaaag
     2161 ttaaatttca gcgaccagcc agagatggtg gtcgtgcccg tattggtgat aatgatgttc
     2221 gcgccgaagc cgccgggcca ctgattcgtc actgtgtaat ggacgctaca cggacctccc
     2281 tgcgaaatcg gcgtagaggt cgccgttggc tgccgcgtcg gggtcgccgt cggagtcgaa
     2341 ccgcgagtcg gggtcggcgt cggcggattg cctccaccga tagtcaccga ataggccgtc
     2401 gtattcagcg tgcc
//
LOCUS       DASZYN010000841         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1281325, whole genome shotgun sequence.
ACCESSION   DASZYN010000841 DASZYN010000000
VERSION     DASZYN010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281325"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            704..2053
                     /locus_tag="VHE33_20970"
     CDS             704..2053
                     /locus_tag="VHE33_20970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99984.1"
                     /translation="MGQSNWPNRFVIARSVAEIFFPQRPQLGLDQRNCSAAVLDKIVS
                     ANAEHKSTAKAKCMLWKLAEIKVSVPHIRDLTAMIGKELHDHQRQQADAHANQQLKPE
                     YREPPKVVSVSVDGGRIMTRADAGRGVHEPAWKETKNACLLTMSSFVSQEDPHPQLPA
                     CFAERDYVEKLVREIHSCTMKSAAKCGEGGSFSEEVGADPATLPSVEASPSRARKAEN
                     WRPRRLVRTCLSSMACSDDFGPLVAGEAQRRGFYQAERRAFLGDGQAWNWTLQDRCFP
                     NFVGITDFVHPLGYVYDAAQVLAPADPWPVYLRASEACWQGRVGDFLTELRAWQTAHP
                     VLGDEALPDQDPRAIIQTTITYLENNRARMNYPDYRRAGLPVSSAMIESLIKEINYRV
                     KGTEKFWNRPDGAEHILQVRAAALGDDDRLSQWILNRPGSPFYRRSTADQDPLACAA"
BASE COUNT          496 a          668 c          728 g          463 t
ORIGIN      
        1 cggcagtcga tctgccagcc ggcgcgcgcg agagccctgc cgaagggcgc cacgcgataa
       61 cggtagcaga cgtggtccgg gccttctgtt aaggccaaga gcttcaaggg tgttcgtcct
      121 ccctgccgtt cttgctctta ctccctctcc cttgggaaag ggctggggcg agggcggttg
      181 gcgcccacgc tggttgccca tgtccagatg tgccgacaac ggcggcgcgg aatcttaaca
      241 tcctggccca atcttcccaa ggcagaaccc gcgccaaagc gggtcgtcgg gtgcataagg
      301 tgaattgcgc gcggaacgga ttttctgagg aacgtcaatg ggagaattgg cagatttcgg
      361 gtatggtcga gcctgtagct gggtgaattg ggaagcccag agagaaaact agcccgttca
      421 aggatgaaca ggatggctcg cgcaaggatc agtgaacagg aatggcagac gtttgtgagc
      481 gttgcggaac gactcgagat tccggtccga gaactcctgc gagaggatgt gcggttctcg
      541 gtcattgagg cggctgggca taagctgggc agggcggtgg ctcagatcac gaccgaacgg
      601 ttggccttgg cgcgggcgga gcggatgatg gggtctcagc catgtccgac ttgcgggcgg
      661 aagtgcccac tggtccatcg agaacgggaa ctggaaacga tcgatgggcc agtcgaactg
      721 gccgaaccgg tttgtcattg ctcggtctgt cgccgagatt tttttccctc agcgtccgca
      781 attgggattg gaccagcgga attgtagcgc cgccgtcctc gacaaaatcg tgtcggccaa
      841 tgcggaacac aaatcgacgg ccaaagcaaa gtgcatgttg tggaagctcg ccgaaatcaa
      901 agtcagcgtg ccgcacatca gggatttgac cgcgatgatc ggcaaggaac tgcacgatca
      961 ccagcggcaa caagcggacg ctcacgccaa ccaacagctc aagccggaat accgcgaacc
     1021 tcccaaggtc gtgtccgtat ccgtggatgg cggtcggatc atgacacggg ccgatgcagg
     1081 acgcggcgtt cacgaaccgg cctggaaaga gaccaagaac gcctgcctgt tgacgatgag
     1141 cagtttcgtc tcccaggagg atccgcatcc acaattgccg gcctgtttcg ctgagcggga
     1201 ttacgtagaa aaattggtgc gtgagatcca tagctgcacg atgaaatcgg cagcgaaatg
     1261 cggagaaggc ggctcttttt cagaagaagt cggcgccgat ccagccacct tgccgagtgt
     1321 cgaagcgagt ccctcccgtg cccgaaaggc agaaaattgg cgtcctcggc gattagtccg
     1381 cacttgtctc agcagcatgg cttgcagtga cgactttggc ccgttggtgg ctggtgaggc
     1441 ccaacgtcgc ggcttctatc aggcggaacg gcgagccttc ttgggcgacg gtcaggcgtg
     1501 gaactggacg ctccaagatc gctgcttccc gaacttcgta gggattacgg atttcgtcca
     1561 tccgctggga tatgtgtacg atgctgcgca agttctcgcc ccagccgatc cttggccggt
     1621 ctatctccgg gccagtgagg cgtgttggca aggacgcgta ggcgacttcc tcacagaatt
     1681 gcgcgcttgg caaacagccc atccggtact tggggacgaa gcgcttcccg atcaggatcc
     1741 ccgtgccatc attcaaacca ccatcaccta cttggagaac aatcgagcgc ggatgaacta
     1801 ccctgattat cgccgtgcgg gactaccggt ctcctcggcg atgatcgaat cgctcatcaa
     1861 ggagatcaat tatcgggtca agggcacgga gaagttttgg aaccgtcccg acggcgccga
     1921 gcacatccta caggttcggg ctgcggcttt aggcgacgac gaccgactca gccaatggat
     1981 cctcaatcgc cccggttccc ctttttaccg aagatcgact gccgaccagg atccactggc
     2041 ctgcgctgcc taaaaatcaa atcaccttat gcacccatcc cgagtcgagc gacgctcgtt
     2101 agtcattaga agagccgtgt ccgggcaccg atgaatctgc ccctttgggg gccatgcccg
     2161 atatcgaacc ccacagggca agccttgaca gatccttcct ggatgacttc tagtcgttgg
     2221 gccggactat tccgaatacg tcgttcgtca aagggtgctt tgccatgaag ttgctcggtg
     2281 cagaaatctt gcggcggctc ggcgcgggcg aatcgatcgc gtcgctgtgc gccgccgcag
     2341 ggatcacgcg ggccg
//
LOCUS       DASZYN010000842         1835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_439470, whole genome shotgun sequence.
ACCESSION   DASZYN010000842 DASZYN010000000
VERSION     DASZYN010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439470"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1004)
                     /locus_tag="VHE33_20975"
     CDS             complement(168..1004)
                     /locus_tag="VHE33_20975"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HVT99985.1"
                     /translation="MTALEFSLILLISSLAAGLLGALSGLGGGVVIVPLLTVIFHVDI
                     HYAIGASLVSVIATSSGAAAAYVREGFSNIRVGMFLEIATTLGALVGAWLTTRVPTHA
                     IGIVFGVVLLYSAYTALKQHREIITQRPDALALRLRLKGTLPTGPGTHQDYVAQHVPA
                     GFSLMTVAGVLSGLLGIGSGAVKVIAMDQAMRLPFKVSTTTSNFMIGVTAAASAGLYL
                     RSGYMAPSLAMPVMLGVLLGSLIGSRLLVRVHVRTLKIVFALVIVALGIEMIINSLRG
                     TL"
     gene            complement(1089..>1835)
                     /locus_tag="VHE33_20980"
     CDS             complement(1089..>1835)
                     /locus_tag="VHE33_20980"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HVT99986.1"
                     /translation="FGSIADIAKALRDVFVVDGCYSPYRERTHGRPVLNLPASRFLGY
                     AQNHDQVGNRAQGERISHLVNLGRTKIAAALVLTAPFVPMLFQGEEWAASAPFQYFTD
                     HDEELGKLVSEGRRKEFAAFGWKPEDVPDPQSPEAFEHSKLNWSERSQPLHAEMLDWY
                     KRLIALRRSTPDLNDPDLSHVRVQHSEEQKWLVMERGAVTVAFSCAKNLIELQLRPQS
                     EIVLASSGEIRMAEQKITLPPDSVAILKTA"
BASE COUNT          368 a          523 c          613 g          331 t
ORIGIN      
        1 ggggcgcggg ccacggctgt gcgtcagata aaggatgccg ccgatcagta cgatggccgc
       61 cgcgagcagc acgccggaac gcagcatgac cccgaccgag atttcgatgc gctgatcggt
      121 ccattgtccg ggggtggggg agtgcgccga ggaatctcgt gactgtttca caatgtgcct
      181 cgcaggctgt tgatgatcat ctcgattccg agcgcgacga tcaccagggc gaaaacaatt
      241 ttgagtgtgc ggacgtggac gcgcaccagc aggcgcgatc cgatcagcga tcccagcagc
      301 acaccaagca tgaccggcat tgcgagtgag ggagccatgt agccgctgcg caggtagagc
      361 ccggcgctgg ccgcggctgt cacgccgatc atgaaattgc tggtggtggt cgaaaccttg
      421 aaaggcagtc gcatggcctg atccattgcg atcactttga ctgcgcccga tccgatgccg
      481 agcaggccgg atagaacgcc ggccacggtc atcagactga agcctgccgg aacatgctgt
      541 gcgacataat cctgatgcgt gccgggtccg gtaggcagcg ttcccttgag ccgaagccgg
      601 agggcgagcg catccggccg ctgcgtgatg atttcccggt gctgtttaag cgcggtgtac
      661 gcggagtaaa ggagcaccac accgaagacg atgccgatgg cgtgggtggg aacgcgtgtg
      721 gtgagccagg cgccgacgag cgcaccgagc gtggtcgcga tttccaggaa catgcccacg
      781 cggatgttgg agaagccctc gcgcacatag gcggccgccg cgccggacga cgttgcgatg
      841 accgaaacga gcgaggcccc gatcgcatag tgaatatcga cgtgaaagat aacggtgagc
      901 agcgggacga tgaccacgcc gccgccgagg ccgctcaacg cgccgagcag tccggccgcc
      961 agcgaactga tgaggaggat gagagagaac tcgagcgccg tcatcgaaga gacagagtac
     1021 agcgtgcggg ggtcggggta cagtcccgga acgcgtgtga acttcgcagg atcgcggtca
     1081 tcggacgttc atgctgtttt cagaatggct acggagtccg gcgggagagt gatcttctgt
     1141 tcggccattc gaatctcccc ggacgaagcc agaacgatct ccgattgcgg ccgtagttgc
     1201 agctctataa ggttcttcgc gcaggaaaac gcaacggtga cggcaccgcg ttccatgacc
     1261 agccattttt gttcctcgct gtgctggacg cgcacgtggc taaggtccgg atcgttgagg
     1321 tccggcgtgc tccggcgcag agcaatcagg cgcttgtacc agtcgagcat ctcggcgtgc
     1381 aggggttggc tgcgctcgct ccagttgagt ttcgaatgct caaaggcctc tggcgactgc
     1441 gggtcgggaa catcttcggg cttccagcca aaggcggcaa attctttgcg tctgccttct
     1501 gagacgagtt tgccgagctc ctcatcgtga tccgtgaagt actggaaggg cgcggatgcc
     1561 gcccattctt cgccctgaaa caacatgggc acgaagggcg cggtcagcac gagtgcggcg
     1621 gcgattttcg ttcgaccgag gttcaccaga tgggagatcc gttctccttg cgcgcgattg
     1681 cccacctggt cgtggttttg cgcgtagccg aggaagcgtg atgcgggcag attgaggacc
     1741 ggacgcccat gggtgcgctc ccgatagggc gagtagcatc cgtcaacgac gaacacatca
     1801 cgcaacgcct tcgcgatatc cgcgatcgat ccgaa
//
LOCUS       DASZYN010000843         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_883862, whole genome shotgun sequence.
ACCESSION   DASZYN010000843 DASZYN010000000
VERSION     DASZYN010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883862"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VHE33_20985"
     CDS             <1..684
                     /locus_tag="VHE33_20985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99987.1"
                     /translation="LQSKNEGSALRMVNLFGRKKEEERRGPTVLTGPRIPRHSSGWEA
                     LLKHLRNENGLRVLDIGPTSPQNINFLTELGHSVYMADIVHESMREEWKLPPEEAGGP
                     PRFNEAAFFEQNLNFHGRQFDVVLLWATLDYVPAGLIDPLVTRLKDATLRDGKVLAIF
                     HSKPTGPYTAYCRYHLTATNEIEMQESEPYAVQRVYTNRQIEKLFTGWSNYKFYLAKD
                     NLYEVIITR"
     gene            complement(742..1095)
                     /locus_tag="VHE33_20990"
     CDS             complement(742..1095)
                     /locus_tag="VHE33_20990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99988.1"
                     /translation="MLSDSEIRERLGPLNDPAPNPDKNPRGPSAARIVALVVLVLVLW
                     GAGVAVILHLERISPALYDARSGHVYRLSDTRHVAYLTQNQSYVAWGAIGVPAVVTLV
                     VAFLGFRRKKLPLDG"
     gene            complement(1204..>1569)
                     /locus_tag="VHE33_20995"
     CDS             complement(1204..>1569)
                     /locus_tag="VHE33_20995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HVT99989.1"
                     /translation="QGVGFQEALEQSIKGEAMVYSLIDVPIMNDAGRDTGGEHALITL
                     SQETGGKHFYAEYGHISEAFRKISEDLRTQYQIGYYPVQRRTQSDFRRIQVSLKSNSS
                     YTLRYRPGYYATPSPPLDQ"
BASE COUNT          325 a          518 c          446 g          280 t
ORIGIN      
        1 ttacaatcca aaaacgaggg ttctgcgctc cgcatggtca acctgttcgg cagaaaaaag
       61 gaagaggaga ggcgcgggcc caccgttctc accggcccca ggattccacg ccattccagc
      121 ggctgggagg ccctgctcaa acatctgcgc aacgagaacg gcctgcgcgt tctggacatt
      181 ggccccactt ccccgcagaa catcaacttc ctcaccgaac tgggccacag cgtctacatg
      241 gccgacattg tccacgaatc catgcgcgag gagtggaagc tccccccgga agaagccggt
      301 gggccgcccc gcttcaacga agccgccttt ttcgagcaga acctgaattt tcacggccgc
      361 cagttcgacg tcgtgctgct gtgggccacg ctcgactacg tccccgccgg cctgatcgac
      421 ccgctggtga cccgcctcaa ggatgcgacg ctgcgcgacg ggaaggtgct ggccatcttc
      481 cactccaagc ccaccggccc atacaccgcc tactgccgct accacctgac ggcgaccaac
      541 gagatcgaga tgcaggagtc cgagccgtat gcggtccagc gggtctacac caaccgccag
      601 atcgaaaagc tcttcaccgg ctggtccaac tacaagttct acctggcgaa agacaatttg
      661 tacgaagtga tcatcacgcg ctgaacgatc tgccccgact cggaggtctg tgacgcgcga
      721 cctgggatct ggaactgcgg tttacccgtc cagcggaagc ttcttccggc gaaatccaag
      781 gaacgccacc accagcgtca ccaccgccgg gacaccgatc gccccccacg ccacgtagct
      841 ctggttttgt gtcagataag ccacatgccg ggtgtcggac aagcggtaca cgtgccccga
      901 ccgggcgtcg tagagcgcgg gcgagatcct ctccagatgc aggatcaccg cgaccccggc
      961 accccacaga acgagcacca ggaccaccag cgcgacaatc ctggcggcgg aaggtccccg
     1021 cgggttttta tctgggttgg gtgcgggatc gttgagcggg cccagacgct cgcgaatttc
     1081 ggagtcggac aacatggatg aagacgacgc ctctgatcat aacaaccggg ccatccaaac
     1141 ggctcgccag ccgtgcgcag catttttgcc tcgatggcca atcgtcactg tctcgattac
     1201 tgattactga tcgagcggcg gcgacggagt tgcatagtac cctgggcgat accgcaatgt
     1261 atacgaggaa ttggacttga gcgacacctg aatccgccgg aaatcggact gcgttcgccg
     1321 ctgcacgggg taatacccga tctggtactg ggttcggagg tcttcggaga tcttgcggaa
     1381 cgcctcggag atgtgcccgt actccgcata gaagtgctta ccgcccgtct cttgggacag
     1441 ggtgatcagc gcgtgctccc cgcccgtgtc ccggcccgcg tcgttcatga tgggcacgtc
     1501 gatcagcgaa taaaccatcg cctcgccctt gatcgactgt tcgagcgcct cctgaaagcc
     1561 gactccctg
//
LOCUS       DASZYN010000844         2639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_122764, whole genome shotgun sequence.
ACCESSION   DASZYN010000844 DASZYN010000000
VERSION     DASZYN010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122764"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VHE33_21000"
     CDS             complement(<1..875)
                     /locus_tag="VHE33_21000"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HVT99990.1"
                     /translation="MTSRTNRDAQRLIRPLVGRLRRLLQELVRTNSVAVLAGGNETPA
                     QRVLQKFFREQGIRAELYSTDFILRSKFPQVRRNKSYAGRHNLSVRLSGSGRGRSLLL
                     NAHMDTVPQGNRPWTRSPWSGRVEAGRVLGLGSFDMKGGLVANAAVVCALHRAGIRTG
                     GDLLFESVVDEEWGGGGGTIAARLRGDTADACVIPEGTQLEIYRATRGGFVVDLVVEA
                     GDPSAYFSNAEVVSPALALGRLLDWVAKLATRRSRVRPKGAYARFSDPVPVQVLAVEA
                     NRTDPDVPLSVPQQST"
     gene            complement(908..>2639)
                     /locus_tag="VHE33_21005"
     CDS             complement(908..>2639)
                     /locus_tag="VHE33_21005"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVT99991.1"
                     /translation="PSCGKARRHVPVCAPMSRNFYNRYLTLIAGLGGLLYGIDIGIIA
                     AALLYLSRTVDLTLGQTSIIVAAVLGGSMVSSPVAGVLAEWFGRKRMMIVSGLMFVAS
                     VGIIVLSQSFVSLLIGRLLQGMSGGVIAVVVPLYLAECLAPKTRGRGTAVFQVMLTFG
                     IVVAALVGFYFTRQAEHAIALAGGNAVLVRAAENHAWRNMFLTVVYPGAVFFLGAFLL
                     SESPRWLRRKGRTQEAHRALLRSLPEDEANRELEEMAVVLEAARPAGAGGHRESLLQR
                     KYVVPFVLACVVLAANQTTGINSVLQFLVVILRQAGLSATHATQGDVGVKLLNFIMSL
                     VAVALVDRKGRRFLLRLGTGGVVVALFISALLFWRVERQRVDVKDKVVAAITGDTISL
                     PIHQLISGGEDRPMVLSVLYSYGNGNRLAAVSTSDHDPVLVIRAESSEAGARLTIQKA
                     TVGPIPGSATSGWIALCMGLFIAAYAVGPGVVVWLVLSELMPVRIRSTGMGIALLLNQ
                     GVSAGIAALFLPVVGRYGYAPMFLFWSACTVIYFVTATFFIPETKGKTLEEIEIYFAS
                     RGSGHNSATT"
BASE COUNT          558 a          846 c          811 g          424 t
ORIGIN      
        1 gtgctctgct ggggaacgct gagcggcacg tcgggatcgg tccgattcgc ttcgaccgcg
       61 agcacctgaa ccgggaccgg atcggaaaac ctggcgtacg cgcctttcgg gcggacgcga
      121 ctccggcgtg tcgctagttt cgccacccag tccagcagcc ggccgagggc cagcgcggga
      181 ctgaccacct ctgcgttcga aaaataggcc gagggatcgc ccgcttcgac gaccagatcc
      241 acgacgaagc caccgcgggt ggcacgatag atttccagtt gtgtgccctc gggaatgaca
      301 cacgcatctg cggtgtcgcc gcgcaggcga gcggcgattg tgccgcctcc accgccccac
      361 tcctcatcga cgacggattc aaagagcaga tcgccacctg tccggattcc ggccctgtgc
      421 agagcgcaga cgacggccgc gttggccacc agcccaccct tcatgtcaaa ggagccgagg
      481 ccgaggacgc gtcctgcctc cacccgtccc gaccatggag agcgcgtcca tgggcgattc
      541 ccctgtggaa cggtgtccat gtgggcgttc agcaaaaggc tgcggccgcg gcccgaaccc
      601 gaaagccgaa cactgagatt gtgcctccca gcgtacgatt tgttgcggcg aacctggggg
      661 aacttcgagc gcaggatgaa atccgtgctg tagagctccg ctcggattcc ctgttctctg
      721 aaaaacttct gcaacactcg ctgcgccggc gtttcgttgc cgccggcgag aaccgcgacg
      781 ctgttggtcc ggacgagttc ctgaagcagg cgccggagcc gccccaccaa aggccgaatc
      841 agtcgctgcg catcgcgatt cgtgcggctc gtcatctcgt tcggtttttg gttcgacccg
      901 gattttctca tgtagttgct gaattgtggc cagagccacg gctcgcgaaa taaatctcga
      961 tttcctcgag cgtttttccc ttcgtttccg gaataaagaa ggtcgccgtc acgaaataaa
     1021 tcaccgtgca agcagaccag aagaggaaca tcggcgcata accatagcgg ccaacgacgg
     1081 gcagaaacaa agcggcgatt cccgcggaga cgccctggtt caggagcagc gcgatgccca
     1141 tgccggtcga gcggatgcgc accggcatca gctccgacag gacaagccat acaacgacgc
     1201 ccggtcctac ggcataggcg gcgatgaaca ggcccataca caacgcgatc cagccgctgg
     1261 tggcagagcc cggtatgggc cccacggtcg ccttctgaat cgtgagccgg gcgcctgctt
     1321 cgctggactc ggcccgaatc acaagcaccg gatcgtggtc cgatgtggac acagcggcga
     1381 gccggttgcc attcccgtat gagtagagca ccgacaagac catcggccgg tcttccccgc
     1441 ccgaaatcag ctggtgaatg ggcagcgaga tggtgtcacc ggtgatggct gcgactacct
     1501 tgtctttcac atcgacgcgc tggcgttcga cgcgccaaaa gagcaaggca gagatgaaca
     1561 atgcaacgac cacgccgcca gttcccagcc gaagcaggaa acgccgcccc ttgcgatcca
     1621 cgagagccac agccaccagg ctcatgatga aattcagaag cttcacgcca acatcgccct
     1681 gcgtggcgtg ggttgccgat aaaccggcct gcctgaggat gacgacaaga aactgcagca
     1741 cggaattgat gccggtggtc tgattggccg ccagcaccac acaagccaga acgaagggga
     1801 ccacgtattt gcgctgcagg agggattcgc gatggcctcc tgcgccggct ggccgcgctg
     1861 cttcgagcac caccgccatc tcttccagct cgcgattggc ttcgtcttcg ggcagcgagc
     1921 gcaacagggc acgatgggcc tcctgtgtgc ggcctttgcg acgcagccag cgcggcgact
     1981 cactgagcag gaaagcgccg aggaagaaca ccgctcccgg atagacgacc gtgagaaaca
     2041 tgttgcgcca cgcatgattt tcggcggcgc gcacaagaac ggcgttgccg ccggcgagcg
     2101 cgatcgcgtg ctccgcctgg cgggtgaaat agaagccgac cagcgcggcg acgacaatgc
     2161 cgaaagtgag catcacctga aagacggcag tcccgcgccc gcgggttttg ggcgccagac
     2221 actcggccag gtacagaggc accacgacgg cgatgacgcc gccgctcatc ccctggagca
     2281 gacgcccgat cagcagcgat acaaagctct gcgagagaac gataatgccg acgctggcga
     2341 caaacatgag gccgctgacg atcatcatgc gtttgcggcc gaaccactcc gccagcacgc
     2401 cggccaccgg cgaggacacc atgctcccgc ccagcaccgc cgcgacgatg atcgaggttt
     2461 ggcccagggt gagatcgacc gtcctgctca ggtagagcag cgcggcagcg atgatgccga
     2521 tatcgatgcc gtacagaagg ccgccaagcc cggcgattaa cgtgaggtac cggttataga
     2581 aatttcgtga cataggcgcg caaacaggaa catgtcgccg tgccttgccg caggatgga
//
LOCUS       DASZYN010000845         6044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_884789, whole genome shotgun sequence.
ACCESSION   DASZYN010000845 DASZYN010000000
VERSION     DASZYN010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884789"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1582
                     /gene="fliF"
                     /locus_tag="VHE33_21010"
     CDS             <1..1582
                     /gene="fliF"
                     /locus_tag="VHE33_21010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HVT99992.1"
                     /translation="IGNASEQAKQFWSGRTGAQKGFLVGGGAATLLLMVLFVRMIGTP
                     DYKPLFTDLEPSDAQTLSAQLQAQGIPHQTSPDGKTISVPADKLDAARMQTAAQGQPH
                     SGRMGFELFDKMSWGQTEFDEKVTYQRALEGELEKSIGTLADVESARVHLVMPSDSVF
                     IDRQRGAKASVILKLRRGGLSKGAVMAISRLVAGAVDGLSPEDVSIIDADSARALGLA
                     HAGPENSDGEGSSLSDRLISTLEPIVGANKIRASVNVNYDQGTTDESQEKYDPAVSVL
                     LTDQKSEDQAGGSAIPAGVPGTSSNIPKPNADKNPPQPVSTQSSKTESATYGVNKTIT
                     HTITPAGRVARITAAILVDDETVKTVTKGKVSWTRKKRSQEELDKIRDLAQAAIGFDA
                     KRGDTITVENMPFDTDEANIDVPSPTWTMQVQKTVTDYSPILRPVALLGLFILAWLFI
                     LRPVQKHVLSTPRLAAPAEAALPASQSHVLSATVTHELGAGTARAAQLKEQTVELIRQ
                     KPVHTARAVQAWLREEPS"
     gene            1579..2598
                     /gene="fliG"
                     /locus_tag="VHE33_21015"
     CDS             1579..2598
                     /gene="fliG"
                     /locus_tag="VHE33_21015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HVT99993.1"
                     /translation="MSATEIAFTGTRKAAILLSLLGEDAAAPILRNLPDEDLERITEE
                     VSNLGHVPLEVTLQVLEEYNQMMTAQEFIAVGGQDVAVRLLAKAFGENGAKDLVERLG
                     RQEERRSLRPETLRKADPQQLARFLAGEHSQTKALVLSHLETKQASALLMKMEPETRA
                     DCVRRLANLGQFSPEVASKVATVLNRRLRSAGDQTKKTDAGPRSIADLMNRMDPATSK
                     EILDNIEREEPKLAIAIRDLMFTFDNFLEVPEQEMRELMNAIDKNTLMIALKGAGEDL
                     RSHFYRTMSSRAVEMMKEDSEVMGPVRSKDVAKAQAEIVSIARKLEAEGKIVLKSQGE
                     DEYVL"
     gene            2588..3262
                     /locus_tag="VHE33_21020"
     CDS             2588..3262
                     /locus_tag="VHE33_21020"
                     /inference="COORDINATES: protein motif:HMM:NF018358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HVT99994.1"
                     /translation="MSSDIGSIYDVVASLQYRDIAGRPANEGIHPMEMNDRPSHGGRG
                     TADKNRSEAELVARIAKERAEAAAQTEARLRQEMEQKLQTAQMAIAKSVQEFAQQRND
                     YFTKVEAEIVQLSLSIAAKILHREAQVDPMLVATLVRMAAERMRDQSAVTIRVATGRA
                     ASWAQYFSGRPNLDHVQILEDAQLGPDDCLLQTELGSANFSLDAQLKEVEQGFFDLLA
                     LRPSAR"
     gene            3259..4566
                     /locus_tag="VHE33_21025"
     CDS             3259..4566
                     /locus_tag="VHE33_21025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HVT99995.1"
                     /translation="MSRPILAPYLHHLQAGSTLRWTGRINQVVGNLVESNGPFCSVGE
                     SCEIRSESGAAYAGEIIGFRGSTMLSMAADQPRGIRFGDKIVTWGARPSIRVGPDLLG
                     RVIDTTGNPLDGKGDYRAVHSVTIDGSAPLPLDRVPIREPLSCGIRAIDGFLTCGRGQ
                     RIGIFGGSGVGKSTLIGMMARGTAADLTILALVGERGREVGEFLEVLGEEGLRRAVVV
                     ASTSDQSPLLRLRAALAATAVAEYFASLGKNVLLIVDSLTRFAMAQREIGLAAGEPPT
                     AKGYTPSVLNMLARLTERAGNFSEGSITAFYTVLMEGDDQQDPVVDTVRSLLDGHVVL
                     DRNLALHSHYPPISVLESLSRLMSSLVSDQQLAKTNALRLLMAAYARSEDLIRIGAYQ
                     KGTDPVLDSAITNLPVLNAFLQQKPHDLGDLKSTVDRLMALPS"
     gene            4569..5006
                     /locus_tag="VHE33_21030"
     CDS             4569..5006
                     /locus_tag="VHE33_21030"
                     /inference="COORDINATES: protein motif:HMM:NF014144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HVT99996.1"
                     /translation="MAFRFTLASVLGVRESLERLEELALARIQLEMARVRHEIEKTNA
                     DLAEAQRVREESMRKPIPAAQLQSMLHAADAAAERKAKLIESLVLLEKQRGEQMRAYQ
                     AARRGRQVLSDLREQHLEAWEQEQARTQQKVLDDIFASRNQRN"
     gene            5260..>6044
                     /locus_tag="VHE33_21035"
     CDS             5260..>6044
                     /locus_tag="VHE33_21035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVT99997.1"
                     /translation="MRIALPAMTNGMTNPLLTNTQSTIAPGFLSVLSTAALPAPVACV
                     SAGAGKQTDSGAQSPRPNPSAATPQSGANAQNANAGTAASFPLPAVTDPTGPTLATVH
                     AVRSGISEATPLSAQQKRTDDAKPVSTVKTATPDIGVPVPVQPVAVDVVAMAGPSAGK
                     SVTSLSPVDTGAAVVRAEVPAFANAREASAGTTAGGQRLVTSNTAASEMPSQLSAPVT
                     TSSADLRQGQFTGNFRPSIPTEQESASVSSIPQLTIAPAMNTVG"
BASE COUNT         1273 a         1775 c         1901 g         1095 t
ORIGIN      
        1 cattggcaac gcgagtgagc aggcaaagca gttctggtcc ggccggaccg gcgcccagaa
       61 gggattcctt gtgggcggcg gcgcagcgac cctcctgctc atggtcctct tcgtgcgcat
      121 gatcggtacg ccggattaca agccgttatt tacggatctg gaaccgtccg atgcgcaaac
      181 cctcagcgcg caactccagg cacagggcat tccccatcag acgagccccg acggcaagac
      241 catcagcgtt cctgccgaca agctggacgc ggcgcgtatg cagaccgcgg cccagggcca
      301 gccccacagc ggacgtatgg gtttcgaact gttcgacaag atgtcgtggg gtcagacgga
      361 gttcgacgag aaagtcacct accagcgggc gcttgagggc gagttggaga agtcgatcgg
      421 gacactcgct gacgtggaga gcgcgcgcgt gcacctggtg atgccgtcgg actccgtttt
      481 catcgatcgc cagcggggcg caaaagcttc ggtgatcctg aagttgcgac gcggtgggct
      541 gtccaaaggc gcagtgatgg caatctcgcg gctggttgct ggtgcggtcg acggtctctc
      601 tccggaggat gtttctatca ttgacgcgga ctcggcacgc gccctggggc tcgcgcacgc
      661 cggtccggaa aacagcgacg gcgaagggtc ctcactcagc gaccgtctga tcagcacgct
      721 tgagcccatc gtgggagcga acaagattcg cgccagcgtc aacgtcaact atgaccaggg
      781 caccacagac gagagccagg aaaaatacga tccggcagtc agcgtgttgt tgaccgacca
      841 gaagtcggaa gaccaggcag gaggtagtgc gattccggcg ggtgttccgg gaacatcgag
      901 caacattccg aaacctaatg cggacaagaa tccaccacag cctgtgtcga cgcagagttc
      961 gaagaccgaa agtgctacct acggcgtgaa caagaccatc acgcacacca taactccggc
     1021 aggccgcgtg gctcgtatca ccgcggcgat cctggtggat gacgaaacgg taaaaaccgt
     1081 caccaaaggc aaagtctcct ggacgcgtaa gaagcgttcg caggaagagc tggataagat
     1141 tcgcgacctg gcacaagcgg cgattgggtt cgatgcaaag cgcggcgaca ccatcaccgt
     1201 ggagaacatg ccgttcgata ccgatgaagc gaatatcgat gtaccgtctc caacctggac
     1261 gatgcaggtg caaaagacgg tgacggacta ttcgccaatc ctgcgtcccg tagccttgct
     1321 cggcctgttt atcctggcgt ggctgttcat tcttcggcct gtccagaagc atgtgctgag
     1381 cacgccgcgg ttggccgcac ccgcagaagc agccctgccc gcatcgcagt cgcacgtgct
     1441 ctcggcaacg gtgacgcacg aactcggtgc ggggaccgca cgcgctgccc agctcaagga
     1501 gcaaacggtg gaactcatcc ggcagaagcc agtccacacc gcccgggcgg tccaggcatg
     1561 gttgcgtgag gagccgtcgt gagcgcaacc gaaatcgcat ttacgggcac gcgcaaggcc
     1621 gcaatcctcc tttctctgct gggagaagac gcggcggcgc cgatcctgcg aaaccttccc
     1681 gatgaggacc tggagcgaat caccgaagag gtgagcaacc tcggccatgt ccctctggag
     1741 gtcacgctgc aggttctcga ggaatacaac cagatgatga ccgcccagga gttcatcgcc
     1801 gtcggcggtc aggatgtggc ggttcgcctg ctggcgaagg cgttcggaga aaacggagcc
     1861 aaagatctcg tggaacgcct gggacgccag gaggaacgca gatcgctgcg tccggaaact
     1921 cttaggaagg ccgatccgca gcagttggcg cgattcctgg caggggaaca ctcgcaaacc
     1981 aaggcattgg ttctcagcca tcttgaaacc aagcaggcct ccgcgctgct catgaagatg
     2041 gagcccgaga cccgggctga ctgcgtacgt cgcctggcga atcttggaca gttttctccc
     2101 gaggtagcgt cgaaggtggc gacggtgctg aaccgccggc tgcgctcggc aggcgatcag
     2161 acaaaaaaga ccgatgccgg accacgcagt atcgccgatc tgatgaaccg catggatccg
     2221 gcgacctcga aggagattct tgacaatatc gaacgtgaag aaccaaagct ggcgatcgcg
     2281 atccgcgacc tcatgtttac cttcgataac ttcctcgaag tgcccgagca ggaaatgcgt
     2341 gaactcatga acgcgatcga caagaacaca ttgatgattg cgctgaaggg cgccggcgaa
     2401 gacctgcgca gccactttta tcgcaccatg tcgtcgcgtg ccgtcgagat gatgaaggag
     2461 gactccgaag tgatgggtcc ggtgcggagc aaggacgttg ccaaagcgca ggccgagatc
     2521 gtctcgatcg cacgcaagct ggaagccgaa gggaagatcg tgttgaagag ccagggagaa
     2581 gacgagtatg tcctctgaca tcggcagcat ttacgacgtt gtcgcgtcgc tgcagtatcg
     2641 ggatatcgcc ggccgtccag cgaacgaagg cattcatccc atggagatga atgaccggcc
     2701 gagccatgga ggacggggca ccgccgataa gaatcggtcc gaagcggagc tggtggcgag
     2761 gatcgcgaaa gaacgggcgg aggctgcggc tcagaccgaa gcgaggctgc gccaggagat
     2821 ggaacagaag ctgcagacgg cgcagatggc gattgcgaag agcgttcagg aattcgccca
     2881 gcagcgcaac gattacttca caaaggtaga agctgaaatc gtgcagctgt cgctttcgat
     2941 cgccgcaaag atcctgcatc gcgaggcgca ggtggatccg atgctggttg cgacgctggt
     3001 gaggatggcc gcggaacgaa tgcgggatca gtcggcggtt acgattcggg ttgcgacggg
     3061 ccgcgccgcg agctgggcac aatatttttc cggcaggcca aatctggatc atgtgcagat
     3121 ccttgaggat gctcagctcg gccccgacga ctgtttattg cagacagagc tcggatcggc
     3181 aaacttcagt ttggacgcgc agttgaagga ggtggagcag ggcttctttg accttctcgc
     3241 actgaggccc tccgcccgat gagccgaccg attctggccc cttatctcca tcatctgcag
     3301 gcaggctcga cgctgcgttg gaccggacgc atcaatcagg tagtcgggaa tctggtcgaa
     3361 tcgaatggac ccttctgctc tgtgggcgag tcatgcgaga ttcgcagtga aagcggagca
     3421 gcctatgccg gcgagattat cggtttccgc ggatcgacga tgctgtcgat ggcagccgat
     3481 cagccgcgcg gcatccgctt cggcgacaag attgtcacat ggggcgcgcg tccttcgatt
     3541 cgtgtgggtc cggacctgct cggccgcgtc atcgacacta ccggcaatcc gctggacggc
     3601 aagggcgact atcgagccgt acattcggtc accatcgacg gctccgcgcc attgccgctt
     3661 gaccgtgtgc cgatccgcga gccactgagc tgcgggatcc gcgccatcga tggatttctg
     3721 acctgtggac gtgggcaaag aattgggatt ttcggcggga gcggcgtggg caagagcacg
     3781 ctgattggca tgatggcccg cggaaccgca gcggacctga ccatccttgc gctggtgggc
     3841 gaacgcggac gtgaagtcgg cgagttcctc gaggttttag gcgaagaggg cttacgacgc
     3901 gcggtagtgg tcgcctcgac ttcggatcag tcgccgctgc tgcggctgcg tgccgcgctt
     3961 gcggccactg cggttgcgga gtacttcgca agcctcggca agaatgtact gctgatcgtc
     4021 gattcgctga cgcgtttcgc catggcgcag cgcgaaatcg gcctggcggc cggcgaacca
     4081 ccgaccgcaa aaggctatac cccttccgtg ctgaacatgc tggcgcgtct gacggagcgg
     4141 gcagggaatt tttccgaggg cagcatcacg gccttctaca ccgtactgat ggaaggcgac
     4201 gatcaacagg atcctgtcgt cgatacagta cgatcgctgc tggacggcca cgtcgtactg
     4261 gacaggaatc tcgctctgca cagtcactat ccgccgattt ctgttttgga aagcctgagc
     4321 cggctcatgt ccagcctcgt aagtgaccaa cagctggcga aaaccaacgc gctgcgactg
     4381 ctgatggcgg cttacgcgcg ctccgaggat ctgatccgca tcggggccta tcagaagggt
     4441 accgatccgg tgctcgacag cgcgatcacc aacttgccgg tgctgaatgc gtttctccag
     4501 caaaagccgc atgacctcgg agacctgaaa agcaccgtcg atcgactgat ggcgttgccg
     4561 tcctgatcat ggcatttcga ttcactctgg cgtctgtctt aggggttcgc gaaagcctcg
     4621 agcgcctcga agaactggcc ctggcgagaa tccagctgga gatggcgcgc gtccggcatg
     4681 agatcgaaaa gacgaatgca gatctggccg aggcgcagcg ggttcgcgaa gaatctatgc
     4741 gcaagccgat tccggcagcc cagttgcagt cgatgttgca cgcggctgat gcagccgctg
     4801 aacggaaggc gaagctgatt gaatcgctcg tccttctcga gaagcagcgc ggtgaacaga
     4861 tgcgcgcata tcaggctgcg aggcgcgggc gccaggtgct cagcgatctg cgcgagcagc
     4921 acctggaagc ctgggagcag gaacaggctc gaacccagca gaaggttctc gacgatattt
     4981 tcgcttcgcg taaccagcgc aattagttcc tgtcatccct ctgcagacgc ggtctcaacc
     5041 gagaccgcgt tttgcctttt tgccccttgc tctgcccatt ggcctccact cggcaaacgt
     5101 tgcccagccg acgcggcaag cgttgccggg aatgactgcc gcagagcaga gtgactgtgg
     5161 tttgttgccc tcaagtcctc tcaaatcaga gctgatatcc cgcgcgggcc gttgggccag
     5221 gcggcccgat cgtggcgacc ggcttgcata aatagcggcg tgcgaattgc cctgccagcg
     5281 atgacgaacg gcatgaccaa cccgctgctg accaacacgc agtcgacgat tgctccggga
     5341 tttctctccg tgctctcgac ggctgcactg ccggcaccgg tcgcgtgcgt ctccgccggc
     5401 gcaggcaagc agaccgattc gggcgcacaa agtccacggc ctaatccttc cgcggcgaca
     5461 ccacagtccg gtgccaatgc gcagaatgcg aacgcaggta cggccgcgtc ttttcccctt
     5521 cccgcggtca cagaccctac aggccctacc ctcgcgacgg tccatgcagt tcggtcaggg
     5581 atcagtgagg cgacacccct gtcggcacag cagaagcgaa ccgatgacgc aaaaccagtc
     5641 tccacagtga agacagcgac tcctgatatt ggggtgccag ttccggtgca accggtcgca
     5701 gtcgatgtcg ttgcaatggc tggcccgagt gcaggaaagt cagtcacttc gctttcgccc
     5761 gtggataccg gggctgccgt cgttcgagcc gaagttccag cctttgcgaa tgcgcgtgaa
     5821 gcgtcggcag ggaccaccgc tggcggtcag cgattggtta cgagcaacac tgcggcgagt
     5881 gaaatgccct ctcagttgtc ggcacccgta acgacgagca gtgcggatct gcggcagggt
     5941 caattcaccg ggaatttccg gccatcgatt cccactgagc aggaatcagc ctctgtctct
     6001 tccatacccc aattgacgat tgcgccggcg atgaacaccg ttgg
//
LOCUS       DASZYN010000846        19833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_188890, whole genome shotgun sequence.
ACCESSION   DASZYN010000846 DASZYN010000000
VERSION     DASZYN010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 19833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19833
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188890"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="VHE33_21040"
     CDS             <1..1231
                     /locus_tag="VHE33_21040"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HVT99998.1"
                     /translation="GTAQGSTFAVPRSENHVARQRTNPATRYSGPSRAKKMGRMDPLH
                     SKHGPTRWDAVSLGEVMLRLDPGEGRIHTTRNFSVWEGGGEYNVARGLRRCFGLRTAI
                     VTALADNPVGRLVEDLMLQGGVDTSLLRWTPYDGVGRTVRNGVNFVERGFGVRAAAGC
                     SDRGHTAIAHTARESWNWEETFGPGRAGETGGARWFHTGGIFAALSESTPAVAEAAMD
                     AARKFGTPISYDLNFRDSLWKSIGGKAKAQEVNRELVRKVDVLLGNEEDFSAMLGIAL
                     KGVTEEFAELHPESYEQMLRDVAAAYPNLKVVATTLRTAHTATRNAWGAMALAEDKIA
                     HIPQTDVEILDRVGGGDSFASGLIYGLLSGKPLEWAVRCGVAHGALAMTTPGDTSMAT
                     LAEVERVMKGGSARIAR"
     gene            1255..1911
                     /locus_tag="VHE33_21045"
     CDS             1255..1911
                     /locus_tag="VHE33_21045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HVT99999.1"
                     /translation="MNSTETKQWIEQLGIMPVLRARSAKEGHALVEALMAGGITVMEV
                     TMTVPGAVGLLRELRKQHGDKLLLGSGTVTDAAQAEETIDSGAEFVVSPSFHSDVVAK
                     TKLMGKVSIPGSLTPTEVITAWRAGADYVKIFPCSAVGGAPYLKALLAPFPELQLIPT
                     GGVTLHTAVDFLKAGARALGVGTDLVNPKAIEEGNPGKVTETARAYLDIVAKFRSSQR
                     "
     gene            1954..2724
                     /gene="nth"
                     /locus_tag="VHE33_21050"
     CDS             1954..2724
                     /gene="nth"
                     /locus_tag="VHE33_21050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HVU00001.1"
                     /translation="MAQKKTAKKGTKKSPRRPTAKEIAKRPPRKGVAGVKGDKGLELD
                     PKRIEDLLRRLQAAYPNAECALRHRNAWELLVATILSAQCTDARVNMVTPELFRKFPT
                     PQAFAQASLPAIEEEIRSTGFYHNKAKSISGAAKKLISDFGGKVPETMAELLTLPGVA
                     RKTGNVVLGVAFGKAEGVVVDTHVLRLSHRLGLTRAEDPKRVEEDLMRIIPRDHWIAF
                     SHEMIHHGRQICIARKPRCIDCPLEKLCNAPDKTWSSH"
     gene            complement(2753..3439)
                     /locus_tag="VHE33_21055"
     CDS             complement(2753..3439)
                     /locus_tag="VHE33_21055"
                     /inference="COORDINATES: protein motif:HMM:NF024741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HVU00002.1"
                     /translation="MKRTRTNYAMVRRLILKDWYLNRWMIFGSIPAGLASLAIVLTGK
                     QVAFMLCIILLCMVIVGVGAQLAMVTTVNERKEQTLAFIMSLPVSWREYTAAKLLANL
                     IIFIIPWLALTAGAIGVLCLPGATHGLIPFTAIMAVEMLITTCLIVVAGVITESQAWA
                     TVGIFCSSLGINGLGYAFAHLRGISAYMWGMQVRWTPTAWTVLIAELLMVPLLLGTAF
                     FVQSRKTDFL"
     gene            complement(3436..4332)
                     /locus_tag="VHE33_21060"
     CDS             complement(3436..4332)
                     /locus_tag="VHE33_21060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HVU00003.1"
                     /translation="MNNLSVCMEGVSKRYPHFQLDNISLDLPQGSILGFIGANGAGKS
                     TTIRILMGLVRQDSGTVRVLGHAMPAEQTAARLEIGFVSEDMRLYGAPTLAWHMNFVS
                     SIFPRWNQAYAETLLRRFDLKAEQKIKGLSHGQRVKAALLLALARQPRLLILDEPTTG
                     LDPVARREVLGELMAVLTDEGRTILFSSHNTLEVEQISDRIAFIDRGRLIAVEEKEDL
                     LDRWRRVRLVVSPGTALPQLPGVVEVGGSDRLPVLTTSQFDPAVISMCQDRGATVQSV
                     DPMTLEEIFVANVQSRRELQLA"
     gene            complement(4319..4735)
                     /locus_tag="VHE33_21065"
     CDS             complement(4319..4735)
                     /locus_tag="VHE33_21065"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HVU00004.1"
                     /translation="MILSQTGGPLYQQIVEQIKQKIAVEEWKAGDEIPSIRQLAADLR
                     VSLITVKRAYLELEREGVIVTQQGKGSFVAPDVGIGPHLYEEKMAGHLEEAARLGSTL
                     GMSEREIVSRLRKALDEVDDDRASFRSPRRSPHEQP"
     gene            4915..6309
                     /locus_tag="VHE33_21070"
     CDS             4915..6309
                     /locus_tag="VHE33_21070"
                     /inference="COORDINATES: protein motif:HMM:NF039847.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HVU00005.1"
                     /translation="MSFTRRRFLQNAGAAALASRARWARAAFVPPSDRITVGVIGWGM
                     IGPTNAKAFLTMNDCQVVAACDLDPARLQMALDTINGAYGNKDCKGYKDYREVLARTD
                     IDAVMIAVPDQSHAIMATEAARAKKDIYGEKPLARTIFEQQAIVRAVEKNNVIWQTGS
                     WQRSLPTFHKAAEIVRNGLIGDVTHVEVGLPGGHHDFPHTLPALMEKLASLPNKPESP
                     AQIVPGTPAWDLAVSDPPADFDYDTWLGPSKKEPYIAVRVFQNWRWNYNTGGGQLMDW
                     IGHHGDIAHWGLGFDHTGPSVVEGHAEFPPENAVWNTATKFTIECLYKKEVTGYPNDV
                     TMTIAGGSPALSMGTKWIGKNGWVWVDRSGFDASNPEWVKEESLPDSERKIQLIVSQE
                     HRRNFLDSVRSRKPTITPVQTAHHSTIPGHLGLISMLTGRKIEWDVKNETILNDPGAS
                     GLLTRPYREPWKLA"
     gene            6382..6954
                     /locus_tag="VHE33_21075"
     CDS             6382..6954
                     /locus_tag="VHE33_21075"
                     /inference="COORDINATES: protein motif:HMM:NF018925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HVU00006.1"
                     /translation="MRESTNGHEEKHVLRGILAGAGAGLAASWVMNLFMTGPGQKLTE
                     SLKSDEEKIQENIDRLTQQQQDRTGEAKEDATMKAADAIVSTTTGGRHLSFEARQKAG
                     PVVHYAFGAVMGGLYGGLAEYSKTVQTGFGTAFGGVLFAGADLVAVPGLRLSPPLSQT
                     QPKSLANPLAAHLVYGAATEILRRAFRAVL"
     gene            7014..8669
                     /locus_tag="VHE33_21080"
     CDS             7014..8669
                     /locus_tag="VHE33_21080"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVU00007.1"
                     /translation="MVWAHVRVRGIAAFLLFAVLAWAGFASAQEASETKPAEPAETHG
                     RLVLVLPFENRTGDKSLDWMGSAFPDVINRRLGSAGFLTIRRSDRIYAFDHLGLPTDL
                     QPTRATAIRIAQTLDADYVVFGHFDKGADATSNQIAAETLTLDVSSLRLSPPIEQPGD
                     AEHLLQMINSLAWKVARQLDPTLSVDEQTFIAADKNLHTDAFRDYIQGVVAVDAKEGI
                     SHLRNALRLDPGYAPAWLALGRAYFADEDYDDAATALGHLPADDPNALEADFYRGLAF
                     FFIGNYREAEDAFAFVSTRLPLPEVVNNQGVAASRRGKDSGALFEQAIASDPRDPDYH
                     FNLAIALAHRNDTAGALKELQEVLNLRPGDSEAQSFANQLKSPAGTGPNPDPGHAVSD
                     AQGPLTRIKRGYSEAGFRQAAFELEQLQQTQLASLSPPDRAAQLIKAGDQFFQRGLVL
                     EAEREYREALKADAQSALAHAGLAAVKERDGESEAARQEAHASIQLAPNVPAYLVLAR
                     LDLQVNQLSAAATDVSEALRVDPESASAKGMKLALERRGQTLP"
     gene            8666..10030
                     /locus_tag="VHE33_21085"
     CDS             8666..10030
                     /locus_tag="VHE33_21085"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HVU00008.1"
                     /translation="MRLAAHHDRHGSHGLLFLLMLLLLFLFCVLGPCTARVAAQTPQV
                     VVLHLDDTIQPISADYLKRGIDRAAEIHAQALLVELNTPGGLLDSTRSMVGDILNSPS
                     PVIFFVAPAGSRAGSAGFFLMESADIAAMAPGTNAGAAHPVVELGKIDDTMKQKILND
                     TLAFLRSYVSKRGRNVDAAQDAVQNSKSYTDQEAQQLHLIDAVAPDDRALLDTIDGRQ
                     VKRFNGMTQTLHTRGANLIAVGPTLREEIMDRLMDPNLAVLILLAGALLIYMEFNTPG
                     TIIPGALGSLLVLLALFSLNLLPVRYTSLALIVGALILMVLEAKFPSHGILAATGTIA
                     LILGTLTLVAGPIPEMRVQWATAIATGVAFGLITTFLVRIAWRARQNKVMIGPEALIG
                     SVGVAQEPLAPRGQILVHGELWLAESQQDPIAPGESVRVRAVDGLRLLVERVQQTVSA
                     GAPH"
     gene            10566..11624
                     /gene="mtnA"
                     /locus_tag="VHE33_21090"
     CDS             10566..11624
                     /gene="mtnA"
                     /locus_tag="VHE33_21090"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HVU00009.1"
                     /translation="MIPTLEWTPEGVRFLDQTKLPLEETYVLATTYQQVADIITTMVV
                     RGAPAIGVSAAMGLALGVKDSPTKTIPDLGADFDTMCRTLAATRPTAVNLFWAIERMR
                     QRFSTLASRPGATTASVRTGLIAEAQRMYDEDIAACRTMGAHGAPLLPKSGGVLTHCN
                     AGALATCGYGTALGVIRSAVENGSQLHVYADETRPFLQGARLTAWELMHDHIPTSVIC
                     DNMAASLMRQGKINAVVVGADRIAANGDVANKIGTYNVAILAREHGIPFYVAAPWSTI
                     DLKTPTGDAIPIEQRPALEVTHHAGKQLTPHGVGIENPAFDVTPAKYVTAIVTERGVL
                     RPPYAESLQELEAATAAS"
     gene            complement(11676..11978)
                     /locus_tag="VHE33_21095"
     CDS             complement(11676..11978)
                     /locus_tag="VHE33_21095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00010.1"
                     /translation="MRYAIFNLRARSLVRGAVLMVGSLVLAFTVGGFPNVHASLLMMV
                     PVALAGWATWETTRCLQRRWSFYHGGVLLLLYADVLVLALVVFLMLYPYAKWLQGV"
     gene            12140..12832
                     /locus_tag="VHE33_21100"
     CDS             12140..12832
                     /locus_tag="VHE33_21100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HVU00011.1"
                     /translation="MTASSNPIASLSPAARRERIVRRVSRELRDGFYVNLGIGLPTLI
                     ANHVPPGIEVVLQSENGMLGVGPYPVAGQEDPDLINAGKETVTALPGTVYFSSADSFA
                     MIRGGHVDLSILGAMEVDEEGTLANWMIPGKMVKGMGGAMDLVASARRVIVAMEHCTK
                     EGAPKILKKCTLPLTGVRVVDTIATDLAWICVTPQGLVVEELAPGFTLEDVQSVTEPK
                     LIASPNLKEMAA"
     gene            complement(12910..14094)
                     /locus_tag="VHE33_21105"
     CDS             complement(12910..14094)
                     /locus_tag="VHE33_21105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00012.1"
                     /translation="MATAGLNRMMLWGDFEGRRLEDRWLLGRLVRPEGRRAWFEAIGP
                     DGLPVMLSITETLNDEEDLIERLRAAAKVRHPNVVAIREAAISHLDDVPVVVAEMEMT
                     EENLGDVLRDRVLTAAEGRQVLDAVLAALGAMHAHGLVHKHVEAASVVAIGETIKLRS
                     DCVSMTGTAFGAAAAEDVRWAARMVAHAVTGRFPANENDPVLQLLPESMARAVRRAMS
                     GIAGATEVAALAGTKLEIARDPNRIDPFSKVAKFSVTPKVDGASVEAPKEASKADTSQ
                     VKVEKPPMTVAAPMQVEEKPNAAPAPRVIAMRPADVPAQGALFGDPLMLHAQDEDRII
                     VDDEPQMSRWPKAPYVIAAAIAVVLVTVFTLYGLLHGQSTTPQQQAPATAAEVVPSAH
                     GH"
     gene            14478..15356
                     /locus_tag="VHE33_21110"
     CDS             14478..15356
                     /locus_tag="VHE33_21110"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HVU00013.1"
                     /translation="MAKKPSPAPASAEPVIVNPRWLAIAVALTLLLAALCGYGALCLL
                     FYQGQWQLLYHPSRTLSTTPADEGMRFDDVHFDVNSAGQSQLDGWWIPASPAAPYPSV
                     TILFLHDAHGSLSDCNPALATLHTAGVNVFAFDYRGFGRSAGSHPTERLATADALAAW
                     TYLTDLRHIPPRSLIVYGDGVGATFAAHVAARFAPAGIILQDPNRSARQIFLTDARAR
                     IVPLWLLQTENLDPTADLAATHIPRLFLDIHGDSARTRTLFAASSYPKQYVDLRFAPE
                     TTVLTAVRRFLDDVLH"
     gene            15360..16517
                     /locus_tag="VHE33_21115"
     CDS             15360..16517
                     /locus_tag="VHE33_21115"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HVU00014.1"
                     /translation="MRPIAIAVLVALSSALGAQNPAFRELTITTGPSPRWIAVADVNL
                     DNNPDIVVANAGSDNADTGTISILLGDGKGAFQAAPGSPFPAGHLPNDIAIADMNHDG
                     NPDLVVANHQSPYLRVFLGDGHGGFRLAPGSPVDVHSNPHPHGVVVADFNSDGMPDAV
                     TDSWGNNQIELLLGDGAGRLISPGRFFATGRRPYERLRLADFNRDGHPDIVTTNLDDG
                     TVSILLGDGRGGLQNAPGSPLAAGAKPWEIAVDDLNGDGISDLITIPYQRDIAGPAEN
                     AVTLLQGDGSGGFHPMSGSPLPLGECRGPNSVAAGNLSGYGSHTIAVACAESKRLLLY
                     RRGADGRFTSSPMPIRGGWGSVALAPLSREGGSAIITANADAGSITIYFPK"
     gene            16732..17139
                     /locus_tag="VHE33_21120"
     CDS             16732..17139
                     /locus_tag="VHE33_21120"
                     /inference="COORDINATES: protein motif:HMM:NF037895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00015.1"
                     /translation="MPQIDIDFEVFKELTNRRPTEDVTYNDVLRELLKLPKPTGKSTK
                     PGTVLGSKPWIVSDTTFPVGSEFIADHKGKTYSGIVKDGKLELSDGSKFTTPSAAAMH
                     ITGTNVNGWRFWKCKLPGTSHFLLLERLRGRAH"
     gene            17389..19479
                     /locus_tag="VHE33_21125"
     CDS             17389..19479
                     /locus_tag="VHE33_21125"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HVU00016.1"
                     /translation="MTEKPQPPFLKCAIADGLVKFTGEGKTERIQYLAANYTARFSDP
                     EEKVRAEFWAELIYKYEYRPERIRFEVKVPRRTPNDLADLVIYGDRDEELKAPYFVFE
                     CKRADVSDAEFTQSIEQACGNRANLAALFCGAIAGLTRRFLRFDKFPPGERDRNILPD
                     IPVRYGKPSDWRFYKNVPGRDLVAIPREELRAAIRKCHQTLWEGGRRSPIAAFGEFCK
                     IIFVKHRDEKNRERKDGEPYTFQRRSEETAAELAVRIHRLYAYEQQLEPDVFTDKINV
                     DPPILAQVVEHLEGISLDRTELDTKGVAFEEFMGGFFKGDFGQYFTPRELIGFAVEIL
                     NPERKQLVLDPACGSGGFLLYALDHVRREADRRFPKRLTDPQQSVEHWTYWHDFAEKN
                     LFGVEINEELARVAKMNMIIHDDGHTNIVGHDALDFFERPLDSEGNPKGEKRPYLYDR
                     NKTLREEKFDIILTNPPFGSVVKRSEKGEGYLEQFDLRNYIGKGMTGTDESDLSESDA
                     KRGAKSAKERASIKTEILFLERVHSFLKPGTGRVAIVLPDGILTNSSLQGVRDWLLSH
                     FQLLAVVSLPQFAFAHYDAGVKASIVFLRRLTEDETVPDDAPIFMALAENIGYDATGR
                     KTFEVTVEKDVPGVERVELLHCDLFDFRVYYEWSTANPKKPDWSERHREIIPDTGLVK
                     QWRSFEKDPKPFFV"
     gene            19515..>19833
                     /locus_tag="VHE33_21130"
     CDS             19515..>19833
                     /locus_tag="VHE33_21130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00017.1"
                     /translation="MKRGEILGRIDVNFWRLTPLFLETFSRPLFPVVQIGEIVTLVQY
                     GCSALATEEPVGVPMLRMNNLQNDGWDLRDLKYIALDADEVARYRLEAGDLLFNRTNS
                     KELV"
BASE COUNT         3983 a         6707 c         5454 g         3689 t
ORIGIN      
        1 gggtacagcc cagggttcga cattcgccgt tccgcgctcc gagaatcacg tcgcccgcca
       61 gcgcaccaat cccgcaacac ggtacagcgg gccatcccgc gcgaagaaaa tgggacgcat
      121 ggaccccctc catagcaagc acggaccgac ccgctgggat gcagtgagcc tcggcgaagt
      181 catgctccgc ctcgaccccg gcgaaggacg catccacacc acgcgcaatt tcagcgtgtg
      241 ggaaggcggc ggcgagtaca acgtcgcgcg cggcctccgc cgctgctttg ggctgagaac
      301 ggccatcgtg acggctctcg ccgacaaccc ggtcggccgc ctcgtcgaag acctcatgct
      361 tcagggcggc gtcgacacat ctctgctccg ctggacacct tacgacggcg tcggacgcac
      421 cgtgcgcaat ggcgtcaact tcgtcgagcg gggctttgga gtgcgcgccg ccgctggctg
      481 ctccgatcgc ggccacaccg ccatcgctca caccgcgcgc gaaagctgga actgggaaga
      541 aacctttgga cccggccgag ctggggaaac cgggggcgcg cgctggtttc acaccggcgg
      601 tatcttcgcg gcgctttcgg aatcaacccc agccgttgcc gaggccgcta tggatgcggc
      661 tcgaaaattc ggcacgccca tctcctacga cctgaatttc cgtgactcgc tttggaagag
      721 catcggcggc aaggcgaagg cgcaggaagt gaatcgcgag ctggtgcgca aagtcgacgt
      781 tctgctcggc aacgaagagg acttctcggc gatgctgggc atcgcgctga aaggcgtcac
      841 cgaagagttc gcagaactgc acccggagag ctatgagcag atgctgcgcg atgtcgcagc
      901 ggcgtatccc aacctcaagg tcgtagccac caccctgcgg accgcacaca cagcaacgcg
      961 caatgcctgg ggcgcgatgg cgctggccga ggacaagatc gcgcacatcc cacagaccga
     1021 tgtcgaaatc ctcgaccgcg tcggcggcgg cgactcgttt gcctcaggac tgatctacgg
     1081 cttgctgagc ggcaagccgc tggagtgggc cgtacgctgc ggcgtcgcgc atggagcgct
     1141 ggccatgacc acccccggcg ataccagcat ggcgactcta gccgaagtgg agcgcgtgat
     1201 gaaaggcggc tcggcccgca ttgccaggta gccgcgaccc caggagagat cgcagtgaat
     1261 tcgacggaaa caaaacaatg gattgaacag ctcggcatca tgccagtact ccgtgcgcgg
     1321 tccgccaaag aaggccacgc cctggtcgag gccctgatgg caggcggcat cacggtcatg
     1381 gaagtcacga tgaccgtgcc cggcgccgtg ggcctgttgc gcgagctccg aaagcaacac
     1441 ggcgacaagc ttctcctcgg ctcgggcacc gtcaccgacg cagctcaggc agaagagacc
     1501 atcgactccg gcgctgagtt cgtcgtcagc cccagctttc attccgacgt ggtagcgaag
     1561 acgaagctga tggggaaagt gtccatcccc ggatcgttga cgcccaccga agtgatcact
     1621 gcgtggcgcg ccggcgccga ctacgtcaag atctttccct gctccgcggt tggcggcgct
     1681 ccttacctca aagcgttgct ggcgccgttt ccggagctgc aactgatccc taccggcgga
     1741 gtgactctgc acaccgcagt ggactttctg aaggccggcg cgcgtgccct tggcgtggga
     1801 accgacctcg tgaatcccaa agcgatcgaa gagggaaacc caggcaaggt caccgaaaca
     1861 gcgcgcgcct atctggatat cgtggcgaag tttcgttcct cgcagaggtg atgcggccca
     1921 tcgcagttcc catgactcga tacaatggcc gccatggctc agaagaaaac tgcgaagaaa
     1981 ggcacgaaaa agtcccccag gcgcccgacg gcaaaagaga tcgcaaagcg gcccccgcgc
     2041 aaaggcgtgg ccggcgtcaa gggtgacaaa ggcctcgagc tcgatccgaa gcgcatcgag
     2101 gatctgctcc gccgtctgca ggctgcttac cccaacgcgg agtgcgccct ccgccaccgc
     2161 aacgcctggg agcttctggt cgccaccatc ctctcagccc agtgcaccga tgcgcgcgtc
     2221 aacatggtca cgccggagtt gttccgcaag tttcccaccc cccaggcctt cgcccaggcc
     2281 tcgctgcccg ccatcgaaga ggagattcgc tcgacgggct tctatcacaa caaggcgaag
     2341 tccatctccg gcgcggcgaa gaagctgatc agcgatttcg gcggcaaggt tcccgaaacg
     2401 atggcggagt tgctgaccct gcccggcgtc gcgcgcaaaa ccggcaacgt cgtcctcggc
     2461 gtcgctttcg gcaaggcgga aggtgtcgtg gtcgacaccc acgtgctacg cctctcccac
     2521 cgcctcggcc tcactcgcgc cgaggatcca aagcgagtcg aagaggacct catgcgaatc
     2581 atcccgcgcg accactggat cgccttctct cacgagatga tccaccacgg ccgccagatc
     2641 tgcatcgcgc gcaagccccg ctgcatcgac tgcccgctcg agaagctctg caatgcgccc
     2701 gataaaacct ggagcagcca ttaatttgct ggcgaggccg tgaggcagcg gctcacagga
     2761 aatcggtttt gcgcgactgg acgaagaatg ccgtccccag cagaagcggc accatcagca
     2821 gttcggcaat cagaaccgtc cacgccgtcg gtgtccagcg gacctgcata ccccacatat
     2881 aggcagaaat gccgcgcaga tgagcgaaag cgtacccgag gccgttgatg ccgaggctgg
     2941 agcagaaaat cccgaccgtg gcccacgcct gcgattcagt gatgactccc gcgaccacga
     3001 tcaggcaggt ggtaatcagc atctccaccg ccataatcgc tgtgaacgga ataagaccat
     3061 gggttgcccc gggaagacag agcaccccaa tcgctcctgc agtaagtgcc agccagggaa
     3121 tgatgaagat gatgagattg gcgagcagct tggcggccgt gtattcgcgc caggagacgg
     3181 ggaggctcat gataaacgcc agtgtttgct ccttgcgctc gttgacggtt gtaaccatcg
     3241 ccagttgcgc gcccactccc acgatgacca tgcacagaag aatgatgcag agcataaagg
     3301 cgacctgttt tcccgtgagc acgatggcaa gcgatgccag cccggcgggg atggaaccga
     3361 agatcatcca gcggttgaga taccagtcct tgaggatgag gcggcggacc atcgcatagt
     3421 tggtgcgcgt gcgtttcatg cgagttgaag ttcccttctg ctctgaacat tggcgacgaa
     3481 gatctcctcc agagtcatcg gatcgacgga ttgcaccgta gccccgcgat cctgacacat
     3541 cgatatcact gcaggatcga actggctcgt ggttagtacg ggtaaccggt ccgatccgcc
     3601 gacctcgacg actcccggga gctgcggcag agcggtaccc ggtgaaacca ccagacggac
     3661 ccgccgccag cgatcgagca gatcctcttt ctcctccaca gcaatgagcc gtcccctatc
     3721 gatgaacgcg atgcgatccg atatctgttc gacctcgagt gtgttatggg aagagaacag
     3781 gatcgtgcgc ccctcatccg tgagcacggc catcagttcc ccgagaacct cccgccgagc
     3841 caccggatcc agcccggttg tgggttcgtc gagaatcagc agtcgcggct gacgcgccag
     3901 agcaagaagc agagccgcct tgacgcgctg cccgtgggag agccccttga tcttctgctc
     3961 tgctttcaaa tcgaaccgcc gcaacagcgt ctccgcataa gcctggttcc agcgcggaaa
     4021 gatcgacgac acgaaattca tgtgccaggc gagcgtcggt gcgccgtaaa gccgcatatc
     4081 ttccgacaca aacccaatct cgagtctcgc cgcagtctgt tcggcgggca tcgcatgccc
     4141 gagcacgcgc accgttccgc tgtcctggcg caccagcccc atcagaatcc gaatcgttgt
     4201 ggacttgcct gcgccgttgg ctccgataaa tccgagaatg cttccctgcg gcaagtccag
     4261 gctgatgttg tcgagctgga agtgcgggta acgcttgctt accccttcca tacagacact
     4321 aaggttgttc atgaggactc cttcggggac ttcggaaaga cgcccgatcg tcatcgacct
     4381 cgtccagagc tttgcgcagg cgcgacacaa tttcgcgctc gctcatgcca agcgtcgatc
     4441 ccagccgtgc agcttcttcc aaatggcctg ccatcttttc ctcatacagg tgcggaccga
     4501 tcccaacatc tggcgccaca aatgagccct tgccctgctg ggttacgatc acgccttccc
     4561 gttccagctc gaggtacgcg cgcttcaccg tgatcaggct cacccgcagg tcggccgcca
     4621 gctgccgaat cgacggaatc tcgtcgccgg ccttccactc ttcaaccgcg atcttttgtt
     4681 tgatctgctc cacgatctgc tgatacagcg gtccgccggt ttgcgaaaga atcatgctgg
     4741 aggcgccgct gtgcatattg aatatactga gtatatacag acactctacg tcgtcaacag
     4801 gaaaaactag gtcaccactg ctaacgccgg gaacgtgccg cgcaatgcta gactgccgtc
     4861 ggtcgtggtt cggctgcgca gcagccgcgg ccagtcagct tccggaggct ggacatgtct
     4921 tttacccgca gacgattcct tcaaaacgca ggagccgcag ctctcgcgtc ccgcgcccgc
     4981 tgggcgcgcg cagcattcgt tcctccctcc gatcgcatca ccgtcggcgt gatcggctgg
     5041 ggcatgattg gccccaccaa tgccaaagcc ttcctcacca tgaacgactg ccaggtcgtg
     5101 gccgcctgcg acctcgatcc ggctcgcctg cagatggcgc tcgacaccat caacggcgcc
     5161 tacggcaata aggactgcaa gggctacaag gactatcgcg aggtcctcgc ccgcaccgac
     5221 atcgacgctg tcatgatcgc cgtgcccgac cagtcgcacg ccattatggc caccgaggcc
     5281 gcgcgggcaa agaaagatat ctacggcgaa aagccgctgg cccgcaccat cttcgagcag
     5341 caggcgatcg tccgcgccgt cgagaagaac aacgtcatct ggcagaccgg ctcgtggcag
     5401 cgttcgcttc ccactttcca caaagccgcg gagatcgtcc gcaacggtct catcggcgac
     5461 gtcacgcacg tcgaagtcgg cctgcccggc ggccatcacg attttcctca cactcttccc
     5521 gctctcatgg agaagctcgc ctccctgccc aacaagccgg agagccccgc acagattgtc
     5581 ccaggcactc ccgcctggga tctcgctgtc tccgatccgc ctgccgactt cgactacgac
     5641 acctggctcg gcccctcgaa gaaagagccc tacatcgccg tgcgcgtctt ccagaactgg
     5701 cgctggaact acaacaccgg cggcgggcag ctcatggact ggatcggcca ccatggcgac
     5761 atcgcacact ggggtctcgg attcgaccac accggtcctt ccgttgtcga aggtcatgcc
     5821 gaatttccac ccgaaaacgc cgtctggaac acggctacga aattcaccat cgagtgcctc
     5881 tacaaaaagg aagtcaccgg ctatcccaac gatgtgacta tgaccatcgc cggcggctcc
     5941 cctgcccttt cgatgggcac gaaatggatc ggcaagaatg gctgggtgtg ggtcgaccgc
     6001 agcggattcg acgcctcgaa tcccgaatgg gtaaaagaag aatcgcttcc cgactccgag
     6061 cgcaaaattc agctcatcgt ctcgcaggag catcgtcgca acttcctcga cagcgtgcgt
     6121 tcgcgcaaac ccaccatcac ccctgtccaa acggcccatc actccacaat ccccggccat
     6181 ctcggcctca tctccatgct caccggccga aaaatcgaat gggacgtgaa gaacgaaacc
     6241 atcctcaacg accccggagc gagcgggttg cttacccggc cctatcgcga accatggaag
     6301 ctggcatagc gcagcgcacg cctccggcga acgcggtctc gcccaaatgc atctcctcaa
     6361 gagacgacac gaggagatgc catgcgcgaa tcgacgaacg ggcacgaaga aaagcatgtt
     6421 ttgcggggaa tactcgccgg ggccggagcc ggccttgcgg catcctgggt catgaacctc
     6481 ttcatgaccg gccccggcca aaaactgact gagtcgctga aatccgatga ggaaaagatt
     6541 caggagaaca tcgatcgcct gacgcagcag cagcaggatc gcaccggtga ggcaaaagaa
     6601 gacgccacca tgaaggccgc cgatgccatc gtcagcacaa ccacgggcgg tcgtcatctt
     6661 tctttcgaag ccagacaaaa agcaggtcct gtggtccatt acgcctttgg agccgtcatg
     6721 ggcggccttt acggcggcct cgcggaatac tccaaaaccg tgcaaacggg cttcggcacg
     6781 gccttcgggg gcgtactgtt cgcgggcgcc gacctcgttg cagttcccgg tttgcgtctt
     6841 tccccgccgc tctcgcaaac tcagccgaag tctcttgcca acccgctggc ggcgcacctg
     6901 gtgtacggcg ccgcgaccga gattttgcgc agagccttcc gagcagtcct ttaatcggtt
     6961 ccgctgccgg ccttagccat cactcgtcgg cgggcgcgac cttataatca gcagtggtgt
     7021 gggcacacgt aagagttcgc ggaatcgccg cattccttct gttcgcggtc ctggcatggg
     7081 cgggctttgc ctcggcgcag gaagcctccg agaccaagcc cgcggaaccc gctgaaacgc
     7141 acgggcggct cgttctggtg ctgcctttcg aaaaccgtac cggcgacaag agccttgact
     7201 ggatgggctc ggccttcccc gacgttatca atcgccgcct cggctccgcc ggctttctca
     7261 cgattcgccg cagcgatcgc atctacgcct ttgatcacct tggcttgccg accgatttgc
     7321 agccgacgcg tgccaccgcc attcggatcg cgcaaacgct cgacgctgac tatgtggtgt
     7381 ttgggcactt tgacaagggt gccgatgcca cttcgaatca gattgccgcc gagaccctca
     7441 ctctcgatgt ctcctccctt cgcctcagcc cgcccattga acagcccggc gatgccgaac
     7501 acctgcttca gatgatcaac agtctggcct ggaaagtggc acgccagctg gatccaacgc
     7561 ttagcgtcga cgaacagacc ttcatcgctg ccgacaagaa tttgcatacc gacgcctttc
     7621 gcgactacat ccagggagtc gtggccgtgg atgccaaaga gggcatcagt caccttcgca
     7681 acgcactcag gctcgatcct ggctatgcgc cagcatggct ggcactcggt cgcgcttact
     7741 ttgccgatga agattacgac gatgccgcta cggccctcgg ccatctgccg gccgacgatc
     7801 ccaacgcgct cgaagctgac ttctatcggg gcctggcgtt tttcttcatc ggcaactatc
     7861 gcgaagccga ggatgcattc gcctttgtca gcacgcgcct cccgctccct gaagtcgtca
     7921 acaatcaggg cgtagccgcc agccggcgcg ggaaagacag cggcgcgctt tttgagcaag
     7981 ccatcgcctc cgatccgcgc gatcccgatt atcacttcaa cctcgccatc gctctggctc
     8041 atcgcaatga caccgccgga gcgttgaagg agctgcagga agtgctgaac cttcgccccg
     8101 gagacagcga ggcgcagtct ttcgccaacc agctgaagag tcccgcgggc accgggccta
     8161 acccggatcc agggcacgcc gtctccgatg cccagggccc cctcacgcgc atcaagcgag
     8221 gctacagcga ggccggcttc cgccaagccg ccttcgaact ggaacagttg cagcagacgc
     8281 agctggcttc cctttcgccg cccgaccgcg ctgcgcagct catcaaagcc ggcgatcaat
     8341 tcttccagag aggactggtc ctcgaggccg aacgggagta tcgtgaggct ctgaaggcgg
     8401 atgcgcagtc ggccctggcc catgcaggcc tggcggcggt taaagagcgc gacggcgagt
     8461 ccgaggctgc ccggcaggaa gcgcatgcgt cgatccagct ggcgcccaat gtgccggctt
     8521 acctggtact ggcgcggctc gacctgcagg tgaatcagct cagtgccgcc gccacggatg
     8581 tcagcgaggc cctgcgcgtg gatccggaga gcgccagcgc gaaaggcatg aagctggcgt
     8641 tggaacggcg aggtcagaca ttgccatgag actggcggct caccacgatc gtcacggaag
     8701 ccacgggctc ctgttcctgt tgatgctcct cctgctcttc ctgttctgcg tccttggacc
     8761 ctgcaccgcg cgggtggcag cgcagactcc tcaggtcgtc gtcctccacc tcgacgacac
     8821 gattcagccc atcagcgccg actatctcaa gcggggcatc gatcgcgccg ccgaaattca
     8881 cgcccaggct ctgctcgttg aactcaacac ccccggcggt ctgctcgatt ccacgcgcag
     8941 catggtcggc gacatcctta actccccatc gcccgttatc tttttcgtcg cgccggcagg
     9001 cagccgtgcc ggttcggccg gcttcttcct catggaatct gcggatatcg ccgccatggc
     9061 ccccggcacc aatgccggcg ccgctcatcc cgtcgtcgaa ctgggcaaga tcgacgacac
     9121 gatgaagcag aagattctca atgacaccct tgccttcctg cgttcctatg tctcgaaacg
     9181 cgggcgcaat gtcgatgcgg ctcaggatgc ggtgcagaac tcgaaatcct acaccgacca
     9241 ggaagcgcaa cagctccatc tcatcgacgc cgtggcgccc gatgaccgcg cgctgctcga
     9301 taccatcgac ggacgtcagg tcaagcgctt caatggaatg acacagacgc tgcacacgcg
     9361 cggagccaac ctcatcgccg ttgggcccac tctgcgtgaa gagattatgg atcgcctcat
     9421 ggatccaaac ctcgccgtgc tcatcctgct cgccggtgcg ctcctcatct acatggagtt
     9481 caatacgccc ggaaccatca ttcccggcgc gctcggatcc ctgctcgtcc tgctcgcgct
     9541 gttctccctc aacctgcttc cggtgcgcta tacatcgctg gcgctcatcg tcggcgccct
     9601 catcctcatg gtgctcgagg cgaaatttcc cagccacggc attctcgcgg ccaccggcac
     9661 gatcgcactg attctgggaa cgctcaccct cgtcgccggc cccattccgg aaatgcgcgt
     9721 gcagtgggcc actgccatcg ccaccggcgt tgcctttggc ctcatcacga cattccttgt
     9781 ccgcatcgcc tggcgcgccc ggcaaaacaa ggtcatgatt gggcccgaag ccctgatcgg
     9841 ctcggtcggc gtcgctcagg aaccgctcgc gccacgcggc caaatcctcg tccacggcga
     9901 actctggctc gcagaatcgc agcaggatcc catcgccccc ggagaatccg ttcgcgttcg
     9961 cgctgtcgat ggactcaggt tgttagtgga gcgagtgcag cagactgtct ctgccggagc
    10021 accacactaa agcctcttcc cggccaacga agtatttctg caaccctcga aaccgacggt
    10081 ctcagtactg gtgggcgtgt tcgcctgctg cctcgccgga ttcctgctca tcttcccgat
    10141 cacggtgccg gatcgcaccg ccgcgccgtc tcaaaatccg ttcttcgcca cagattgcgc
    10201 actgccatgg ataccttccg agcaacggca gaatgctctc gcgccagaat ccgcggcgcg
    10261 ctatccgagc catttctcgt tgcccgcact tcgggcattt caacttcatt ttggtcctgt
    10321 gtcccaaaga tcgcggcggc gtcaactcag ctcctgtccc tctttgtgtg cccaaccggt
    10381 tgtgtcagac ccatgcatcc gcaagcgcgg gccaaaggag tttggctcca gtaactttta
    10441 tgaaactgaa gggtaacagg ttgcctcgtg gatctatcgg gaatctccat cttttcaatc
    10501 acctgcaagt tactaaagtt gtcccgttgt agtgcaccca aagttccctg gttaaactaa
    10561 gagcaatgat tcccaccctc gaatggaccc cggaaggcgt ccgcttcctc gaccagacga
    10621 agcttcccct cgaagaaacc tacgtccttg ccaccacgta tcagcaggtg gctgacatca
    10681 tcaccaccat ggtcgtccgc ggtgcgcccg ccatcggcgt ctccgccgcc atgggcctcg
    10741 ccctcggtgt caaagacagc cccaccaaaa ccattcccga tcttggcgcc gacttcgaca
    10801 ccatgtgtcg caccctcgcc gccacccgcc ccaccgccgt caacctcttc tgggccatcg
    10861 agcgcatgcg tcagcgcttc tccactctcg cctcgcgacc cggcgccact accgccagcg
    10921 tccgcaccgg cctgatcgct gaagcccagc ggatgtacga cgaggacatc gccgcctgcc
    10981 gcaccatggg cgctcacggc gctcccctcc tccccaaatc cggcggcgtc cttacccatt
    11041 gcaatgccgg cgccctcgcc acctgcgggt acggcactgc cctcggcgtc attcgctccg
    11101 ccgtcgaaaa tgggagccag cttcacgtct acgccgatga aacccgtccc ttcctgcagg
    11161 gtgcgcgcct caccgcatgg gagctgatgc acgaccacat tcccaccagc gtgatttgcg
    11221 acaacatggc cgcgagcctg atgcgccagg gcaagatcaa tgccgtcgtc gtcggcgccg
    11281 accgcatcgc ggccaatggg gacgtcgcca ataagatcgg tacgtataac gtcgccatcc
    11341 tcgcccgcga gcacggtatt ccgttctacg ttgcggcccc gtggtcgact atcgacctca
    11401 agactcccac cggcgacgcc atccctatcg aacagcggcc cgccctcgaa gtcacccatc
    11461 acgcgggcaa gcagctcact ccgcacggag tcggtatcga gaatcccgcc ttcgacgtca
    11521 cgcctgccaa atatgtaacg gcgattgtta cagaacgcgg ggtcctgcgc cccccgtacg
    11581 ccgaatccct ccaggaactc gaggccgcga ccgccgcaag ctgagccctg cgctctgcga
    11641 cctgtgccct gaattgcggg ccctgcgcct gcagttcaca ccccctgcag ccacttcgca
    11701 tacggataca gcatcaggaa taccaccagc gccaacacca gcacgtccgc atagaggagc
    11761 agaagcaccc cgccgtgata gaagctccat cgccgctgca ggcatcgcgt cgtctcccat
    11821 gtcgcccatc cggccagcgc caccggcacc atcatcagca acgacgcgtg tacgttcggg
    11881 aacccgccca cggtaaacgc caggaccagg ctccccacca tcagcaccgc tccccgaacc
    11941 agcgaccttg cccgtagatt gaagatggca taccgcatcg gccaatcacg agtctatcca
    12001 aatccggggg ccattttgac tgccctgctt tcggcgctca ggaccggagt ggccagggag
    12061 ccggttttgg ctcgatggga tggtagcggg gccatcgcgc aaagcgcgat cccctgcgta
    12121 tattgaagtc ggtccaccga tgacagcttc ttccaatcca atcgcctctc tctcccccgc
    12181 agcccgccgc gagcgcatcg ttcggcgcgt ctcccgcgaa ctgcgcgacg gcttctatgt
    12241 caacctcggc attggcctgc ctacactgat cgccaaccat gttccgccgg gcatcgaagt
    12301 cgtgctgcag tcggaaaacg gcatgctcgg cgttggtcca tatcccgtcg cgggccagga
    12361 agaccccgac ctcatcaatg caggcaagga aaccgtcacg gctcttcccg gcacggtcta
    12421 cttctcctct gccgattcct tcgccatgat ccgcggcggc catgtcgatc tctccatcct
    12481 cggcgctatg gaagtcgacg aagaaggcac gctcgccaac tggatgatcc ccggcaaaat
    12541 ggtcaagggc atgggcggcg ccatggacct ggtcgcaagc gcccgccgtg tgatcgtcgc
    12601 tatggagcac tgcacgaaag aaggcgcgcc caagatcctg aagaagtgca ctctcccgct
    12661 caccggcgtc cgcgtcgtcg acaccatcgc caccgacctc gcctggattt gcgtcacccc
    12721 ccagggatta gtcgtcgaag aactagcccc gggattcacc ctcgaagatg tccaaagcgt
    12781 gacggagccg aagctcatcg ccagcccaaa tctcaaagaa atggccgctt aggccccagg
    12841 gctcgccagg aacgcagaac gggctgcaca aaattcgtgc agcccgtcta aacctgcctt
    12901 cacaaaaact cagtgcccgt gcgcgctcgg aacgacttcc gccgctgtcg ccggagcctg
    12961 ttgctgtggg gtggtggact gtccgtgcag cagcccgtac agcgtaaaga ccgtcaccag
    13021 cacaaccgca attgccgccg caatcacata cggagccttt ggccaccgtg acatctgcgg
    13081 ctcgtcatcc acgatgatcc tgtcttcgtc ctgcgcatgg agcatcagtg gatcgccgaa
    13141 caacgcgccc tgcgccggca catccgccgg ccgcatggca atcacccgcg gcgccggagc
    13201 cgcattcggc ttttcttcca cctgcattgg ggccgcaacc gtcatgggtg gtttctccac
    13261 cttgacctga gacgtatcgg cctttgaagc ctctttcgga gcttcgacgc tggcaccgtc
    13321 aacctttggc gtcaccgaaa acttcgccac cttcgaaaac ggatcgatgc ggttcggatc
    13381 gcgcgcgatc tccagtttcg tccccgccag agcggccact tcggtcgccc cggcaatccc
    13441 gctcatcgct cgccgtactg cgcgcgccat cgactccggc agcagctgca acaccggatc
    13501 gttctcgttg gccggaaaac ggcctgtcac cgcatgcgcc accatgcgcg ctgcccagcg
    13561 cacatcctcg gcagccgcgg ctccaaaggc cgtgcccgtc attgagacac agtcgctccg
    13621 cagcttgatg gtctcgccaa tcgccaccac gctggccgct tccacatgtt tgtgtaccag
    13681 tccatgcgcg tgcatcgcac ccagcgctgc cagcaccgca tccagaacct gccggccctc
    13741 ggctgcggtc agcacgcgat cgcgcagcac gtcgcccagg ttctcctcgg tcatttccat
    13801 ttcagccacg accaccggaa cgtcgtccag atgcgagatc gccgcctcgc gaatggccac
    13861 cacattcgga tggcgaactt tcgccgccgc gcgcagccgc tcgatcaggt cctcttcgtc
    13921 gttcagcgtc tcggtgatgc tcagcatcac gggcaagcca tccgggccga tcgcctcgaa
    13981 ccatgcgcgc cgtccttcgg gccgtaccaa acgccccagc agccagcggt cttcgagtct
    14041 gcggccttca aagtctcccc acaacatcat gcgattcagg cctgctgttg ccatagtctt
    14101 gcttcctcct gcgggctttc gagcgcttat gccgtcagcc gcacacacta ctctcccgtt
    14161 attccaagta aaccgcgtcc tgggggcaat ccacgccgaa ttttcgcgcg tgtacaaaag
    14221 ttggtacgaa tctgcaccgt caattccaga tcacgcacga ctcgtgccgg ggcaggcgcg
    14281 ccgccaactg agggacggtg ctaggctcga cgtaacaatg tccacaccgc cgcacacccc
    14341 agccacatcg caacccgtga agctttggtg cccggtctgt gcgcgcccgg tcgaagaccc
    14401 cctcgtttgt ggcgactgct ccgccgtgat ctgccgtgtc tgcggcacgc ccctcgaatc
    14461 tcccgacgag ctcgcctttg gctaagaagc catcgcccgc gccggcctcc gccgagcccg
    14521 ttatcgtgaa cccgcgctgg ctggccatcg ccgtcgcact cacgctgctg ctcgcggcgc
    14581 tctgcggata cggtgctctc tgtctgctct tttaccaggg ccagtggcag cttctctatc
    14641 atccgtcgcg caccctgtcg acaacacccg ccgatgaagg catgcgcttc gacgacgtcc
    14701 acttcgatgt gaacagcgcc ggtcagtccc agctcgacgg ttggtggatt cccgcatctc
    14761 cagccgcccc ctacccttcg gtgaccatcc tctttctgca cgatgcccac ggctcgctgt
    14821 ccgattgcaa ccccgccctc gcaaccctcc acaccgctgg cgttaacgtc ttcgccttcg
    14881 actatcgcgg cttcggccgt tccgccggtt ctcatcccac cgaacgtctg gccaccgccg
    14941 acgccttggc cgcatggacg tatctcaccg atctccggca catcccgcca cgcagcctca
    15001 tcgtctacgg cgacggcgtg ggcgcaacct ttgcggccca cgtcgccgcg agattcgcgc
    15061 ctgcgggcat cattttgcag gacccgaatc ggtccgcccg ccagatcttt ctcaccgatg
    15121 cccgtgcgcg gattgtgcca ctgtggctgt tacagacaga gaacctggat cccacggccg
    15181 atctggcagc cacgcacatc ccgcgactct tcctcgacat tcatggcgat tcggcccgca
    15241 ctcgcaccct gttcgccgcc tcctcgtacc caaagcaata cgtcgatctg cgattcgctc
    15301 ccgaaacgac ggttttgacg gcagtccggc gtttcctcga tgatgtcctg cactgattca
    15361 tgaggcccat cgccatcgcc gtgctcgtcg cgctcagcag cgcactcggt gcgcagaatc
    15421 ccgctttccg cgaactcacg atcaccactg gccccagtcc ccgctggatc gcggtcgctg
    15481 acgtcaacct cgacaacaat ccagacatcg tcgttgccaa cgccggcagc gacaacgcgg
    15541 acaccggcac catcagcatc cttctcggag acggcaaagg agcattccag gcggcacccg
    15601 gttcgccctt tcctgcaggc cacctcccaa acgacatcgc gatcgccgat atgaatcacg
    15661 acggcaaccc ggatctcgtt gtcgccaatc atcagtcgcc gtacctgcgc gtctttctgg
    15721 gagatggtca tggtggcttt cgactcgcgc ctggatcacc cgtcgatgtc cactccaacc
    15781 ctcatcctca cggcgtcgtg gtggccgact tcaactccga cggcatgcct gacgccgtca
    15841 ccgacagctg gggcaataac caaatcgagt tgctcctggg cgacggcgcc ggccggctca
    15901 tcagcccagg caggtttttt gccaccggcc gccgccccta cgagcggctt cgccttgccg
    15961 atttcaaccg cgatggccat ccggatatcg ttaccaccaa cctcgacgat ggaaccgtct
    16021 ccatcctcct cggcgatgga cgcggcgggc tccagaacgc acccggctcc ccgctggctg
    16081 caggcgcaaa gccctgggag attgcggtcg atgatctgaa cggcgacggc atcagcgatc
    16141 tcatcacgat tccttatcag cgggacatcg ccggtccggc cgagaacgct gtcaccctcc
    16201 tccagggcga cggctccggc ggcttccacc ccatgtccgg ttcgcccttg cccctgggcg
    16261 agtgccgcgg ccccaacagc gtcgctgccg gcaatttatc cggctatgga agccatacca
    16321 tcgctgtcgc ctgtgcggaa agcaagagac tcctgctcta ccgccggggc gcagacgggc
    16381 gttttacctc atctccgatg cccatccgag gcgggtgggg atcggttgcc ctcgcacccc
    16441 tttcgcgcga aggcgggagc gcgatcatta ccgccaatgc cgacgcgggc tccatcacga
    16501 tctattttcc aaaatgaaac cccgcgggcc tgccccacca acggttgacg ggtaggggga
    16561 tagcagccgg gaaaagtgca cccccattct cgtatcggaa taccgaaggg tcgggttgga
    16621 caggggaagt catccccgaa aagcaaaccc ttctattagg attgggaata gttctgcaac
    16681 gtgctatagt tctcctgagc actcttaacg atccacaagg agagcaccaa gatgccccag
    16741 attgacattg attttgaggt gtttaaggaa ttaaccaacc gccgccccac ggaggacgtc
    16801 acttacaacg atgtgcttcg agagctgtta aagctgccga agccaacggg gaaatccaca
    16861 aaacccggta ctgtcctcgg aagcaagcct tggatcgttt ccgacacgac tttcccggtt
    16921 gggtccgaat ttatagcgga ccacaaaggc aaaacctact ccggtatcgt caaggacggg
    16981 aaactggaac tcagcgacgg cagtaaattc accactccct cggctgcggc catgcatatc
    17041 acggggacaa acgtaaacgg atggcgcttc tggaagtgca agctcccagg tacatcacat
    17101 tttttacttc tggagaggct gcgcggaagg gcgcactagc agttgtgggt ccagtctctc
    17161 gcgtacaaga cgacgtttca aaaacggaat agagatccca gcggcgattc ggtggaaggg
    17221 catcaaggga aaatatcgga acccttcctt gaaggtgcac cagaggcagt gcccccagcg
    17281 ggttaaactg aagcgactca acaatctcag gggtaatgga ccccaacaac cgaaaactga
    17341 gacgactcag caatccgcgc catcacgtat tccatcaggt gaaatcttat gaccgaaaaa
    17401 ccccagcctc ctttcctcaa gtgcgccatt gccgacggtc tcgtgaagtt cacaggcgaa
    17461 gggaaaaccg agcgcattca gtaccttgct gccaactaca cggcccgctt ttccgacccg
    17521 gaagaaaaag tgcgcgcgga gttctgggcc gaacttattt acaaatacga ataccgtccc
    17581 gagcgcatcc ggtttgaggt caaagtccct cgccgcacgc cgaacgatct cgccgacctt
    17641 gtcatctacg gcgacaggga tgaggagctg aaggcacctt attttgtctt cgagtgcaaa
    17701 cgagccgacg tttccgacgc cgagtttacc caatccatcg agcaagcctg cggaaaccgc
    17761 gccaatcttg ccgcgctgtt ctgcggcgca attgccggtc ttacccgccg cttcctgcga
    17821 tttgacaaat ttccgccagg cgagcgcgac agaaacatac ttcccgacat tcctgttcgc
    17881 tacggcaagc cctcggactg gcgcttttac aagaacgtcc caggccgtga cttggttgct
    17941 atcccacgtg aagagctccg cgccgcaatt cgaaaatgcc accagactct ttgggaaggc
    18001 ggacgtcgca gcccgatcgc agccttcggc gagttctgca agatcatctt cgtcaaacat
    18061 cgtgatgaga agaaccgcga gcggaaagac ggcgagcctt acacgttcca acgacgtagc
    18121 gaagaaaccg ccgccgagct cgccgttcgc atccacaggc tctatgccta tgagcagcag
    18181 ttggagccgg atgtctttac ggacaagatc aacgttgatc ctcccatcct tgcgcaagtt
    18241 gtcgagcact tggaaggcat ttcactagac cgcacggaac tcgacaccaa gggcgtcgcg
    18301 ttcgaggaat tcatgggcgg ctttttcaag ggcgacttcg ggcaatactt taccccgcgc
    18361 gaactcatcg gctttgccgt cgagatcctt aacccggaga gaaaacaact cgtcctggac
    18421 cctgcctgcg gttccggcgg cttcctgctt tatgcgcttg accacgttcg ccgggaggcc
    18481 gaccgtcgtt ttccgaaacg ccttactgac ccccaacaga gcgtcgagca ctggacctat
    18541 tggcacgatt ttgcggaaaa gaaccttttc ggcgtcgaaa ttaatgaaga gttggcccgc
    18601 gtcgccaaga tgaacatgat tatccatgac gacggccaca cgaacatcgt tggccacgat
    18661 gccctggatt tctttgaacg tcctctggat agcgagggca atccgaaggg tgaaaagcgc
    18721 ccttatcttt atgatcgaaa caaaaccctc cgtgaagaaa aattcgacat catcctgacc
    18781 aacccgcctt ttggctccgt cgtgaaacgc agtgaaaagg gggagggcta cttagaacaa
    18841 ttcgatttac ggaattacat cggcaagggc atgactggca cggatgaatc cgacctcagc
    18901 gagagcgacg ccaagcgtgg tgcgaaatcc gcgaaagagc gtgccagcat caagaccgaa
    18961 atcctcttcc tagagcgcgt acattccttc ctcaagcccg gcaccggtcg cgtcgccatc
    19021 gttctgccag atggcatcct aaccaattct tcactgcaag gtgtgcgcga ctggctgctc
    19081 tcgcatttcc agcttctagc cgtcgtctcc ctccctcagt ttgccttcgc ccattacgac
    19141 gccggagtga aagccagcat cgtattcctt cgccgcttga cagaagacga aacagtccca
    19201 gacgacgcac ccatcttcat ggccctcgca gaaaatatag gctacgatgc cacaggcagg
    19261 aaaaccttcg aggtgaccgt tgagaaggac gtgccgggcg tggagagggt ggagctcctg
    19321 cactgcgacc tcttcgactt ccgcgtctat tatgagtgga gcaccgcgaa ccccaagaaa
    19381 ccggactgga gcgagcgaca ccgtgagatc attcccgaca ctggcctagt caagcagtgg
    19441 agatcgttcg aaaaggaccc aaagcctttt ttcgtctaag ccctgtggag cggagcgaag
    19501 ccttgtgctt cggcgtgaaa cggggcgaaa tcttggggcg gatcgacgtt aatttctggc
    19561 ggctcacccc gctctttctc gaaacattca gccgtccgct ttttcctgtc gtccaaatcg
    19621 gcgagatagt gacgctggtg caatatggat gctcggccct tgcgacggag gagccagtcg
    19681 gtgtgccgat gctgcggatg aacaaccttc agaatgacgg ttgggatttg cgcgacctaa
    19741 aatacatagc gttggatgcg gacgaggtgg cgcgttaccg actcgaagcg ggcgacctgc
    19801 ttttcaatcg tacaaattca aaggagcttg tcg
//
LOCUS       DASZYN010000847         2727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_1091629, whole genome shotgun sequence.
ACCESSION   DASZYN010000847 DASZYN010000000
VERSION     DASZYN010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091629"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1987
                     /gene="istA"
                     /locus_tag="VHE33_21135"
     CDS             77..1987
                     /gene="istA"
                     /locus_tag="VHE33_21135"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HVU00018.1"
                     /translation="MAHVLEMARIQAIQQLHAAKWSQRRIARELGIDRGTVARCLRAL
                     QSEPKPAIPPTGSDGSKAATFEGAPPPPADPNAGSEDADHSGVAKAAIPPTGEVGSIE
                     PVLAPSNPPSHGAHAAGRRSDCEPFRELILAKLDQGLSAQRIYQDLTSEHSTAVGYNS
                     VKRFVRRLRTTTPLPFRRMECAAGEEAQVDFGTGARLVGSDGKQRKSYVFRVVLSHSR
                     KGYSEATRRQTTEDFFRCLENAFSQFGGVPKTLIIDNLKAAVAHPDWFDPVLTPKVQS
                     FCQHYGTVILPTKPRTPRHKGKIEAGVKYVKNNGLKGRTFTNVEEENRFLLDWEENVA
                     DRRIHGTTKRQVAKVFEESERPALLPLPRERFPCFQEAKRTVHRDGHIEVAKAYYSVP
                     PEYLGHELWARWDARLVRIFNHRWEQVAIHVRTEPGRFDTQDGHVAEEKISGLERGAG
                     YLLGKVSAVGPRTQQWAQAMVTARGIQGTRVLQGLLSLAKKHASETLEEACEIALSYG
                     CFHLRTVRRLLDRQAAKQELLPFLEEHPIIRPLDDYAAVVRQAIHRQDGRSSMSEGFS
                     RHDRTKASGGEAQKKSLATPSPASQGVTDLLPSRSGYPSSGCSSAEPDSVSPDTPSVV
                     PPSVSHQEKTDE"
     gene            1980..>2727
                     /gene="istB"
                     /locus_tag="VHE33_21140"
     CDS             1980..>2727
                     /gene="istB"
                     /locus_tag="VHE33_21140"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HVU00019.1"
                     /translation="MNDRLLTMLKRLRLSGLAQSLDVRLQEAAGHQLNHAEFLELILQ
                     DELLVRDERQIARRVKAADFRELKPLDDFDWSFNPSINRKQLYDLATCRFVRERRDVL
                     FLGPPGVGKSFLVQALGQQAIKQGFLVYYRSIFDAVRDFLHEQALADDKVLAKYLKPD
                     LLIMDDMGMKQLPRRSGEFLFEIIMRRYEVRSTIMTSNRPLEDWGKLIGDVPSATAIL
                     DRFLHHAEIVSITGKSYRLRNRAAAEQSNGN"
BASE COUNT          570 a          859 c          816 g          482 t
ORIGIN      
        1 aaaagcagcc attttacgtg cagcgcagtg cgcggttggt tttcatcaac atccccgacg
       61 ttgatggaga ccgcgcatgg cgcacgttct ggaaatggcg cgcattcagg caattcaaca
      121 gctacatgcc gcaaaatggt cacagcggcg catcgctcgt gagctgggca tcgaccgcgg
      181 cactgtggcg cgatgcttac gagcgctgca gtccgaacca aaaccagcca ttccgcccac
      241 cggctcggac gggtcaaaag cagccacttt cgagggcgca ccgcctccgc cggccgatcc
      301 taacgctggc agtgaagacg ccgatcatag tggcgtggca aaagcagcca ttccgcccac
      361 cggggaagtg ggctcgatag agccggttct tgcgccgtcg aatcctcctt ctcacggcgc
      421 gcatgcggct ggccgccgta gcgactgcga gcccttccgc gaactcattc tggcgaagtt
      481 agaccagggg ctgtcggcgc agcgcatcta ccaagacctg accagcgagc actccacggc
      541 cgtcggatac aacagcgtca agcggttcgt tcgccggctg cggaccacga cgccgcttcc
      601 cttccgtcgg atggagtgcg ccgccggaga agaagcgcag gtcgatttcg gaaccggtgc
      661 gcggctcgtc ggctccgacg gcaagcaacg caagagctat gtgttccgcg tcgtcctgag
      721 tcacagccgc aagggctata gcgaagcgac gcggcggcag acaaccgaag acttcttccg
      781 ctgcctggaa aacgccttct cacagttcgg cggcgtgccc aaaacgctga tcatcgacaa
      841 cctcaaagcg gccgtcgctc atcccgactg gttcgacccg gtgctgacgc cgaaagtaca
      901 gtctttctgc caacactatg gcacggtgat cctgcccacc aaaccccgaa cgccccgtca
      961 caaaggcaag atcgaggcgg gcgtcaaata cgtcaagaac aacggcctca agggccgcac
     1021 cttcaccaac gtcgaggaag aaaatcgctt cctactcgac tgggaggaga atgtcgccga
     1081 ccggcgcatt catggcacca ccaagcggca ggtggccaag gtctttgaag aatccgaacg
     1141 cccggcattg ttgccgctgc cgcgcgagcg gttcccttgt ttccaggaag cgaagcggac
     1201 ggtgcatcgc gacggccaca tcgaggtggc caaagcctat tactcggtgc cgccagagta
     1261 tctcggccat gaactctggg cgcgctggga cgcacggctg gtacgaatct tcaaccaccg
     1321 ctgggagcag gtggccatcc acgtgcgaac tgagccaggg cgcttcgata cccaggacgg
     1381 ccatgtggcc gaggagaaga tcagcgggct ggaacggggc gccggttatc tcttgggcaa
     1441 agtcagcgcc gtcgggccgc ggacacagca atgggcgcag gcgatggtga ccgcgcgtgg
     1501 catccaaggc acgcgcgtct tgcaagggct cttgtccctg gcgaagaagc acgccagcga
     1561 aacgctggaa gaagcctgcg aaatcgccct ttcctacggc tgttttcacc tgcggaccgt
     1621 ccgccgcctg ctcgatcgcc aggcggccaa gcaagagctg ctgccgttcc tcgaagagca
     1681 cccgatcatc cgtcctctcg acgattatgc cgcggtggtt cgccaggcga tccatcgcca
     1741 agacggtcgc tcttccatga gcgaaggttt ttcaaggcat gacaggacaa aagcaagtgg
     1801 aggtgaggcg cagaaaaaaa gccttgccac accgtcgccg gcatcgcaag gcgtgacgga
     1861 cttgcttccg tccaggtccg gctatccctc gtcgggttgc tcctcagcag agcccgactc
     1921 tgtttcaccg gacactccca gcgtagttcc accttccgtg tctcaccagg agaaaaccga
     1981 tgaatgaccg actgctcacc atgttgaaac ggcttcgcct ctcgggcctc gcgcaatcgc
     2041 ttgacgtgcg gctgcaagaa gcggctggcc atcagctcaa tcacgcagag ttcctcgaac
     2101 tgatcctgca agatgagctg ctggtgcgcg acgagcggca gatcgctcgc cgtgtgaagg
     2161 ccgccgactt tcgagagctg aaaccgctgg acgactttga ttggtcgttc aatccctcga
     2221 tcaaccgcaa gcagctctac gacctggcca cgtgccgctt cgtccgcgag cgccgcgacg
     2281 tgctgttctt gggcccgcca ggcgtgggaa agtcgttcct cgtccaagca ctcggccaac
     2341 aagcaatcaa acaaggcttt ctggtttact accgttcgat cttcgacgcg gtccgagact
     2401 tcctgcatga gcaggcgctc gcggacgaca aggtgttggc gaagtatctc aagcccgact
     2461 tgctgatcat ggacgacatg ggcatgaaac agttgccgcg caggagcggt gagtttctgt
     2521 ttgagatcat catgcggcgc tacgaggtcc gcagcacgat tatgacgtcg aatcgccctc
     2581 tggaggactg gggaaaactg attggtgacg tgccgagcgc gacggccatt ctcgaccgct
     2641 tcctgcacca tgcggagatc gtgtcgatca ccggcaagag ctaccggtta cggaatcggg
     2701 ccgccgcgga gcagtcaaat ggcaacg
//
LOCUS       DASZYN010000848         2426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_760676, whole genome shotgun sequence.
ACCESSION   DASZYN010000848 DASZYN010000000
VERSION     DASZYN010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760676"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2266
                     /locus_tag="VHE33_21145"
     CDS             <1..2266
                     /locus_tag="VHE33_21145"
                     /inference="COORDINATES: protein motif:HMM:NF019368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HVU00020.1"
                     /translation="PDLNMPDGESTTRSILIGKRWYQQHYGVDVRIGWNPDSFGYNWQ
                     LPQIYKKTGIDYFVTQKMAWNDTNQLPFKLFWWESPDGSKVLTYFPHDYANGNLNPVR
                     LSVDLEKAREYAPGLTNMMDLFGVGDHGGGPTRAILDEGVHWIQGNKIIPKEQFGTAQ
                     PWFSTVEKKIATDSPVWNYQTIAQGYHPPSPIEGQIHIPTWKSEMYFEYHRGVFTTQA
                     NHKRNMRESPEWTLNAEKYASLAWLDGNPYPGEQLTEDWKKITFNDFHDLAAGSGIGV
                     IYTDAQKDYDAVRWSTNEISAKSLKTVAAHVDTHVAAGVPVLVFNPLAWRRSGDVKVD
                     VQMPAATPDVSVFDAKGAVLPSAVLKKDAATNTFHLLVEAKDVPSLGYEVLHVVPGKH
                     PFTSDLKVSGTTLENAALRVVVDPATGCITSLYDKKTGFETLEKGSCGNELQAFKDTP
                     KDYDAWNIDPGTLDQPPTRLTQADSVQVVEHGPMRAAIRVTRHWQNSKFVQEIQLETG
                     ADQAVVVNDIDWHETHVLLKAAFPLAATSPFATYEIPFGTIQRPTTRNNSWEKAQFEV
                     GAQRFADLGNAQHGFSLINESKFGYDGVGNLLRLTLLRSPTWPDPNADRGHQHFAYAL
                     YPHAGDWKQAMTVRHGYDFNYPLQAMQVEAHTGSLPAEHSFVAISPDDVVLTAMKKAE
                     DSHALVFHFYEWAGRGGTAELRVPPGATAAVETNLMEKPEGDPLPLKGDAVTVHFKPF
                     EVVAVRVDYPESGH"
     gene            2317..2391
                     /locus_tag="VHE33_21150"
     tRNA            2317..2391
                     /locus_tag="VHE33_21150"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2349..2351,aa:Gly,seq:gcc)
BASE COUNT          530 a          721 c          751 g          424 t
ORIGIN      
        1 accggatctg aacatgcccg acggcgaatc cacgacgcgg tcgatcctca tcggcaagcg
       61 ctggtatcag cagcactacg gcgtggatgt gcgcatcggc tggaatccgg actcgttcgg
      121 ctacaactgg caactcccgc aaatctacaa gaaaaccggc atcgactatt tcgttaccca
      181 gaagatggcg tggaacgaca cgaatcagct gcccttcaag ctcttctggt gggagtcgcc
      241 ggatggcagc aaggtgctga cctatttccc ccacgactac gcgaacggca atctgaatcc
      301 cgtgcgcctg agtgtcgacc tggaaaaagc acgagagtac gctcccggac tgacgaacat
      361 gatggacctg ttcggcgtgg gcgatcacgg tggcggcccg acacgcgcca tcctggacga
      421 aggcgtgcac tggatacagg gaaacaagat cattccgaaa gagcagttcg gtaccgcaca
      481 gccgtggttc tcaactgtcg agaaaaagat cgcaaccgat tctccggttt ggaactatca
      541 gacgatcgca cagggctacc atccgccatc gccgatcgaa ggtcagattc acatccccac
      601 gtggaaatct gagatgtact tcgagtatca ccgcggcgtg ttcacgaccc aggccaatca
      661 caaacgcaac atgcgcgaaa gcccggagtg gacgctcaac gcggaaaagt acgcatcgct
      721 cgcgtggctg gacggcaacc cgtatcccgg cgaacagctc accgaggact ggaagaagat
      781 caccttcaac gattttcacg acctcgccgc aggatcggga atcggcgtga tctacacgga
      841 cgcccagaag gactacgacg ccgtgcgctg gtcgaccaac gagatttcgg cgaagtctct
      901 gaaaacggtc gcggcccatg tcgatacgca cgtagcggcc ggtgtgccgg tgctggtgtt
      961 caatccgctg gcttggcggc gttcgggcga tgtgaaagta gatgtgcaga tgcccgcggc
     1021 gaccccggac gtctcggtgt tcgatgcgaa gggtgcggtg ttgccatcgg ccgtcctcaa
     1081 gaaggatgcg gcgacgaata ccttccactt gctggtggag gcgaaggatg tgccgtcact
     1141 gggatatgaa gtgctgcatg tcgtcccggg aaagcatccg ttcacgagcg atctgaaggt
     1201 ctccggaacc acgcttgaga atgcggccct gcgcgtggtg gtcgatccgg caaccgggtg
     1261 catcaccagc ctttacgaca agaagacagg cttcgagacg ctggagaaag gttcctgcgg
     1321 caatgagctg caggcgttca aagacacacc caaagactac gacgcgtgga acatcgatcc
     1381 cggcacgctg gatcagccgc caacgcgact gacccaggcg gactccgtgc aggtcgtgga
     1441 acacgggccc atgcgggcgg cgattcgcgt cacgcggcac tggcagaatt cgaagttcgt
     1501 ccaggagatt caactggaaa ccggcgccga ccaggcggtt gtggtcaacg acatcgactg
     1561 gcatgagacg cacgtgctgc tcaaggcggc cttccctctg gcggcgacca gcccgttcgc
     1621 gacctacgag attccttttg gaacgatcca gcgtccaacg acccgcaaca acagctggga
     1681 gaaggcgcag tttgaagtgg gcgcgcagcg tttcgcggat ctgggcaacg cacagcatgg
     1741 cttcagcctc atcaacgagt cgaagtttgg atacgacggc gtgggcaatc tgctgcgcct
     1801 gaccctgctg cgctcgccga cctggcccga tcccaatgcg gatcgcgggc atcagcattt
     1861 tgcgtatgcg ctgtatccgc acgccggcga ctggaaacag gcgatgacgg tgcggcacgg
     1921 ctacgacttc aactatcctt tgcaggcgat gcaggtcgag gcgcacaccg gatcgctgcc
     1981 ggccgagcat tcctttgtgg ccatcagccc ggacgacgtt gtcctgaccg cgatgaaaaa
     2041 ggcggaggac tcgcacgcgc tcgtcttcca cttctacgag tgggccggta gaggcggcac
     2101 ggccgaactg cgggttccgc ccggtgcaac cgccgcggtg gagacgaatc tgatggagaa
     2161 gccggagggc gacccgctgc ccctgaaagg cgatgcggtc acggtccact tcaaaccgtt
     2221 cgaggtcgta gcggtccgcg tggactaccc cgaaagtggg cattgagacc gattgaccct
     2281 tagcgaagac gaccgatata ctcaggagga acccaagcgg gagtagctca gtggtagagc
     2341 atcgccttgc caaggcgagg gtcgcgggtt caagtcccgt ctcccgctcc agtctcgatc
     2401 aagcgcgatc tgcgctggtt ggctga
//
LOCUS       DASZYN010000849        31974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_886276, whole genome shotgun sequence.
ACCESSION   DASZYN010000849 DASZYN010000000
VERSION     DASZYN010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 31974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31974
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886276"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VHE33_21155"
     CDS             <1..658
                     /locus_tag="VHE33_21155"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HVU00021.1"
                     /translation="KGAFVNGGESSRVANLTRLMSGWSNGVVDLDNDGWKDLVVARSN
                     VMDNIEQISRHFRYAEPNSVFRNLGNGQFADVSATAGADFIRPAPHRGLACGDLDNDG
                     RVDLVITALGGPVSVLRNMTQTRNHWILFKLVGSKSNRMGIGAQIRVTTDDGRKLYNE
                     ATTSTGYAASSDPRVHFGLGDSRLLREIEIRWPSGRRQLLRDVSADRIMVVTEPEGGS
                     "
     gene            718..3315
                     /locus_tag="VHE33_21160"
     CDS             718..3315
                     /locus_tag="VHE33_21160"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVU00022.1"
                     /translation="MSCVPVLFVAAQAAGQLPPSCKPPASAARPPAGTSQAKVYDAVG
                     VWFAQKGDLKCAVAAFDQALRIEPHSAEGHFDLGLVRQRQQQNDAAVEEFRLALQYDP
                     ALLQAHCALGSVLSDPAAAEAEFRRALAVNPQLVCALDGLAQVLLNGGRYDAALDYWR
                     QATQIQPNAPDLQLSVATATYKVAKARQADGLPDLDGSTVANAIGLLTTLLSKHPGMT
                     AAHFTLGNIYANEQRFREAADEYRAVVGQDPTDAVALAAEVKALVDSTAYTDALAPAR
                     AYVHQKPDDSSGHVLLGMVYRGLGEYAEAEPELELGAVKDPGDFEAHYQLGFVLAKLG
                     KPDQALHQLRQAVALNPGDKSAQFQLAAVLRALGQNQEAEKIVEQFRKTTDTDFRNSQ
                     LTSDGIKANDLLQAGKPSEAAEIYRQMLKEKPDSAWTTYNLALALEAMQDTKGAEAAL
                     LKGIEIDPKAARIRAELGALKLTDGDTVSAQQWLQSALDLEPQLAEARGNLARIYAKK
                     GDFATAGKLLHQALEDDPQYKEGHLQLGLLLAHQRKIPEAEQELDRAVALAPRDPNIL
                     STAGKAREEMGKMSESIALLRKVVALRPDLAAAHLDLAVALADSYDLPGALTETNEAV
                     RLAPQSGVVHFYRGRVLYDLGRTAEAQTDFETARQIVPQMPEPLYYLALIDKQEGRFS
                     LASGLLEETVQRQPRNVMAWYLLGQCYEQQAETDKAVAAWRQAIALDSNFSQALFGLA
                     RALRSSDRGESDKFMARYVEIQKQRRILDSAGTLANNGVVAASAHDWPEATSQLKAAI
                     AECGDCAVKGDLHKKLGLIDCQAGDLNNGEKELLTAKGLKPTDPEIQRALQLIAQARN
                     QHSASGKAY"
     gene            3390..5096
                     /locus_tag="VHE33_21165"
     CDS             3390..5096
                     /locus_tag="VHE33_21165"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HVU00023.1"
                     /translation="MLSQMVSGNATDQPVRPLPPGMKAPLVDFRDIAAQAGLTARIIS
                     GDLDQTYIVENTGTGVAIFDYDNDGLPDIFLVQGDRLHNSGPPLTPHLYHNRGGLHFE
                     DVTAKAGIGHLGWGQGVCAGDADNDGHVDLFLTQWGHNVFLHNLGNGTFRDETRERGL
                     YRPESRWSTGCAFIDYDRDGYLDLAVANYVNFKAQDSPPPRTRSGCNWKGMPVPCGPR
                     GLKGETMTLYHNDGHGHFVDVTKQAGIETLPEYYGFTVLTGDFDNDGWPDIFITCDST
                     PSLYFHNKHNGTFEEEGLASGLAVNEDGREQAGMGASAADYDGDGRLDIFKTNFSNDT
                     NTLYRNLGSNNFDDVTSAAGLAVHTQYVKWGTAFIDFDNDGWPDLFIADGHVYPFVEK
                     YNLGEEFKQPRQLFWNRGDGQFFDMSFTGGSGITAKHASRGIAVGDLDNDGSQEIVTV
                     NLFEPPSLLKNFAPRGNALLVRAVTASGRDAVGARLTLAMGGRKEIDEVRSGGYHISQ
                     GDFRVHFGMGHATKADLTIRWPDGPVSNVETIPGVDANQWIVVREGRGIVERHPFTHD
                     SP"
     gene            5271..6641
                     /locus_tag="VHE33_21170"
     CDS             5271..6641
                     /locus_tag="VHE33_21170"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HVU00024.1"
                     /translation="MQGEHFKTTTAHPVASIQDIETPEQKNARMEWFREARFGMFIHW
                     GLYSILAGRWNGKEVPGIGEWIMNRASIPVAQYKALAARFNPTQFSAAGIVGLAKSAG
                     MKYIVITSKHHDGFAMFDSKANPFNIVQATPFKRDPLRELAAECRRQGLKLGFYYSQD
                     QDWTAPGGAAYKTGDHQPPTYHWDPAQNGSFASYLETKAIPQIRELLTNYGEFPAIVW
                     FDTPTTDMTPELAGKIVTVLNEHPKLIWNNRLGGGYQGDTETPEQYIPARGYPGRDWE
                     SCMTMNDTWGYKVDDTNFKSTETLVRNLIDIASKGGNYLLNIGPEATGEVPQPEVDRL
                     HEVGRWLGVNGEAIYATRPTLFGDEAGSFSTTEKDEDGKAKFIPAWKWRSTTKADRIY
                     IHLFEWPGTTFQLTKVPRQVTEAYLLADSSHRSLKVTRTADGVEVALPAEAPDPIASV
                     LVLKTA"
     gene            complement(6680..7069)
                     /locus_tag="VHE33_21175"
     CDS             complement(6680..7069)
                     /locus_tag="VHE33_21175"
                     /inference="COORDINATES: protein motif:HMM:NF016885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HVU00025.1"
                     /translation="MVRTGILNPQINSLLSRVRHTNTLLITDRGFPYWPAIETVDLSL
                     IDDIPRVLDVLRAIRMNFTIGHIVMAEEFRLRNSPALVSSFTEAMSGLEVEFEPHVEF
                     KKRVPQVIGLIRTGDTIQFANMILESS"
     gene            complement(7072..8412)
                     /gene="fucP"
                     /locus_tag="VHE33_21180"
     CDS             complement(7072..8412)
                     /gene="fucP"
                     /locus_tag="VHE33_21180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HVU00026.1"
                     /translation="MTHSSMPLDNSAHSTSGDVPLVPREYKVVFALITTLFFLWAIPN
                     NLNDVLIRQFMKSFVITRFQAGLVQSAFYLGYFVLAMPAALVMRRLGYKSGFLIGLFL
                     FSLGSLLFWPAAEVGRYSFFLVALFIIASGLSFLETASNPFIAQLGDPRTAARRLNLA
                     QAFNPLGSITGVLIGTVFIFSGVELTPGQMAELQSRHAYQAYLHSETLRVIAPYLVLA
                     AVTLTMVVVIASVRFPASLASEEDRGPGSHRAADLMRRPHFVMAVVAQFAYVGAQVGT
                     WSYLIPYIQAYTGEPEKSAGFFLTGSLVLFGAGRFFSAWLMRFVQPSRLMAIYAVINT
                     VLAAMGVLFPGWTGMIALLLTSFFMSLMFPTIFAQGIRGLGETAKLGGSLIVMAIIGG
                     AVLTPMMGLLSIKFGGLAAAYVVPALAYVFIAFYSFADMRLVKSDPAALRAREA"
     gene            complement(8409..9452)
                     /locus_tag="VHE33_21185"
     CDS             complement(8409..9452)
                     /locus_tag="VHE33_21185"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HVU00027.1"
                     /translation="MKAAVLHAPGDVRVENVPDPVRGPGEALLRVRRVGMCGSDLNSY
                     RGRNPLVSFPRIPGHEIAATIVELEPEHPSHLSLGMDVTLSPYKSCGLCPACRNGRPN
                     ACRDNQTLGVQRDGAMGEYLAVPVNKLFVADLSLDELCLVEPLTVGCHAVARAEVKPQ
                     DVVAIFGCGGVGLGAVAASAFRGATTVAMDVDDAKLEIARKAGAVHTIHTEKHDAKEA
                     LAELTGGDGPDVVIEAVGLPQTFRAAVELVAFTGRVVYIGYAKEPVSYETRLFVQKEL
                     NILGARNALPEDFHQVIQMLEQHRFPVDAAISTIAPLDRAPELLRAWSDDPARFTKIM
                     ITLDQEMPSQAIG"
     gene            complement(9481..10458)
                     /locus_tag="VHE33_21190"
     CDS             complement(9481..10458)
                     /locus_tag="VHE33_21190"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HVU00028.1"
                     /translation="MLRSSTTVWASKRASQMQYRALGKTDLEVSVVAFGAAPLGGVYG
                     LTAPEEAKRAVHLAIDEGINFFDVSPYYGKSLAEHRLGTALEGRRNKVILATKCGRYG
                     ANEFDFSAPRIFASIDESLRRLRTDYVDLLLAHDVEFADLTQIVEETIPAMRRLQQMG
                     KTRYIGVTGFPLRALIHIARTSPVDAILSYCHYNLLADDMDAVLAPLAENQGIGLINA
                     SPLHMGMLTEAGPPDWHPAPSEMREAARRAAQFCHDREYDIADLALRFCFDYPRVATT
                     LVGMATREQVRRSLRAFEAPADPQLVRKVRAIFAPVFNRFWPSGREENQ"
     gene            complement(10471..12276)
                     /locus_tag="VHE33_21195"
     CDS             complement(10471..12276)
                     /locus_tag="VHE33_21195"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HVU00029.1"
                     /translation="MQRRDFCRLLAVAAAAKAIPAIGQSAPESSSEVPAGFNEYSQDY
                     ARFCALPPNKRTFYKLSNGKIVPAKLDNADWKATAWGSPEPLPVPGGSWDGVPMESPI
                     AGLSGRGPFKPTWDSLLEYDAPEWYQDAKFGIWAHWSPQCVPEAGDWYARNMYLQDQH
                     QYKYQLEHYGPPSQFGYKDLCAQWTLLNWDPDELIARYKKAGARIFISLANHHDGFDA
                     WDSKHQPWNSAALGPHRDVVGTWAAAARRQGLRFGVTVHQARNWWWFQPSHGADATGP
                     LAGVPYDGDLTAGEGMDEWWQGLDPQRLYGVKHPFDALPDVSYVRNFYDRTRDLIDQH
                     NPDLLYFDNSLLPLGWGGMNIGAYFYNHSLRTNSGRMEAVLNIKEVPDHLVKSVVADF
                     ERGLTSGIQKYPWQSETCIGEWHYNRSLYEKPGEFGGYLPPRDAIHWMIDTVSKNGTF
                     ILNIPGRPDGTIDSKEIAVLDGITAWMQVNGEAIYETRPWKVYGEGPNSVKAGSFQGN
                     SISKLGQKDIRFTRNKANNVVYAIVLGWPAEPIVVQSLGLSASTQPGKVTQVELIGTG
                     ERLNWKQTGEALRVELPRQYRPAVDYAAALKVTLG"
     gene            complement(12411..13451)
                     /locus_tag="VHE33_21200"
     CDS             complement(12411..13451)
                     /locus_tag="VHE33_21200"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HVU00030.1"
                     /translation="MAARLKDIADDLGLSVVTVSKVLRNHPDIGEATRKRVQKRMREL
                     NYQPNFAARSLVTGRTWTLGLVVPDLLHPFFAEIAKAISTETRKHGYSLLISSSDEDP
                     ELEIEEIKQLLARRVDVILVASAQSSVECFRLIEEQKTPYILLDRRFDDLDANFVGVD
                     DASVGMLATSHLYEQGCRRIAHIRGPNVSTAIGRLEGYTRALASHNLNPLPGHVVSLG
                     SSGDQRGEKGGYEAAKQLLAAEARPDGIFCFNDPSALGAMRAILEAGLRIPEDMAVVG
                     CGNFSYSDFLRVPLSSVDQGSETIGKRAADLALKIARKKTPTKPKTEYISSRLVVRAS
                     SRRAPDPSSDDS"
     gene            complement(13685..15256)
                     /locus_tag="VHE33_21205"
     CDS             complement(13685..15256)
                     /locus_tag="VHE33_21205"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HVU00031.1"
                     /translation="MNRREFLVAAGAASLRSTSSLGGWAQDEAPPIEHRLPPTDADYQ
                     RSRSYIEEVPVHRYHWASDRAVEAFRDMKFGLRIHWGIYSIYGRPGESWPFLTLPFDK
                     RQEYNQAFRSWNPREFDADGWMNLCEESGMKMFAFTSKHHEGFSMFDTKTRVRNRTNW
                     TAPGSPRIEDCDLAYSIMETPFRRDVIRELTDSAHKRNIKIDLYFSHSDWYDADFRPY
                     GWHPLQVPSSPRLSGWTPSDGTTQEFWDSMKKRMSGRLVTVPDPTPSEVRRMVERHRA
                     QLTELLTRYGTIDMMCLDIFWGPAVWPQMRETILKLRELQPDVMLRARGIGNYGDYYT
                     PERFVPATKAATKMPWFVIYPLGTSFSYESDGSKYKGTKWIIDNLVDVTAKGGNFMVG
                     VGPSGMGSFHPAAIAQLKEAGQWLRINGEGIYGTRPRAGTDWAEGNNLRFACSKDGRI
                     LYCFVTDWPGESLKIRSLDARPVSSVEMFGYPKKLEFRRDATDNFVISIPTELQDPSN
                     RSSQFAWGLKLSYAT"
     gene            16063..17163
                     /locus_tag="VHE33_21210"
     CDS             16063..17163
                     /locus_tag="VHE33_21210"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HVU00032.1"
                     /translation="MGSFRRTRASLATFLLVSAALATPQLAVTLHAQSTDAAQLDSAD
                     AGLFQQGLDAQHKRDLATAENDYRELLARSSGFLPARFNLGLVLDAEGRTAEALDAFE
                     AVVASDPGFPAVQMFAGIENFRLGRLPAAQTALELATRQSPENLQCWFWLARTDFAAG
                     KTLEGKAAVDKALAISPEEPRVLYLLAQYQISAQDLAGAERTLTGLATRYPKVPDFHE
                     SLGSVYYLEARLDKAEEEYRTQLQLDANNPQAFSMLGVILLDRGQAKQSIEYLAKGLE
                     ANPRIAYLQRKIGQALLESGQALEAVEHLRQATALDPRDATAHFLLWKADSSLNRKTE
                     AAAELEAFRKLQSQPQMPVGLPPTTAQGAVPH"
     gene            17160..18911
                     /locus_tag="VHE33_21215"
     CDS             17160..18911
                     /locus_tag="VHE33_21215"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HVU00033.1"
                     /translation="MRSKTTRRDFLRDLAWLPAASVWPGRVWADAGDPAAKANPYHVE
                     FDDVTAESGIRFVHERAASTEKLVVETMGAGCAWLDYDQDGFLDAFFVNSGATPQFHP
                     ASMPQPALYRNNGDGTFTDVTVRSGIRVNDGFYIGAAVGDFNNDGYPDIYMTGYGKSV
                     LYRNNGNGTFTDITARAGVENAGRWATAAAWFDYDHDGRLDLLVTNYIDYDWNHDPYC
                     GARLPGYRVYCDPFHFRGTSMRLYHNNGDGTFTDRTEQAGLLNPEGKSLGLVIADFDG
                     DGWDDILIGNDGMRSFLYFNNRNGTFRDATYESGAGFGGNGEVESGMGIDAADATGKG
                     RMDFFVCHMDNQPNRFYVNNGDGTFTDRTMNTGFGFSGIRNTSYAVRFVDWDNDGNRD
                     LIVVNGSMLDNIRLYHPDSSYAEAKMLYRNTGEGQFVDASGTQPASFGVARVGRGLAV
                     GDYDNDGNIDFLQSNNGEQAQLYRNLGARGNHWITLKLIGTRSNRDAIGARVQLRAGD
                     FFSFDQVRSGGSYCSSHDLRLHFGLGARENADEIEIRWPSGVTDRLTHLKANQFYAVR
                     EGEGVVPSKFPRFRNRH"
     gene            complement(18920..19555)
                     /locus_tag="VHE33_21220"
     CDS             complement(18920..19555)
                     /locus_tag="VHE33_21220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HVU00034.1"
                     /translation="MQKTIRVLICDDHLLFRLGLRSLLSGEPDIDVIGEAATGRHTIE
                     ACRQLRPDVLLLDLRMPDGGGIEALRTVSSESPETRALILSSYTSEEEIYQALEAGAL
                     GYILKDVGREPLLEAIHAVAAGRRSVPPWMEAILRQRSGRPDLTAREYEVLRLLVSGL
                     TNREIARVLGNSENTVRNHTISIFAKLDVSDRAEAVSAALQRGIIIADEPR"
     gene            complement(19521..21743)
                     /locus_tag="VHE33_21225"
     CDS             complement(19521..21743)
                     /locus_tag="VHE33_21225"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HVU00035.1"
                     /translation="MRLRGAPRSFGGVIVTAVLLFAALVGALALWPNRSYRLPWQDSF
                     ASGDIDGWQAFGGAWSPYKGGIRNNSDERGAKFITGSPAWSDYAVDADVSLLGEDGDA
                     GIIVRSSDEEEGVDSYSGYYVGLRDSNNSLTIGRADHGWAEYQAVSPRERLLPSHWYH
                     LHIVAVGCSIAAVASSGTPGDRTWVAMYEPSCARSGRIGLRSYSSGGLWKNIRVSRAG
                     SGELNPLLAQAPPQPKPGFLQSEAGFNSLRLNQPGISSQFTENLHQAATAPLAPIGQL
                     SGLSAGQPVTVAIRGSVTLISPRVYVQDATGGATVEFLHAPSLKIGDEVQIAGEVEPS
                     HFSAALHKATGHVLWQRSPAPPISVSATQAAVGLYDARFIETEAEVDGVRDSAGGQIE
                     LLLHSGYQHFVSIVDSQANPSPLRNIRPHSRVRVRGVCVVDPAFTGNAAGFAILLRSS
                     SDVHVLAGPPWWALRNLLAAGMALPVVALLGLFVYSRAEHWRMRAVLDERLRMAREVH
                     DTLAQGYAAIAFQLESAIDREGRISTPESVTRALQMAERSRGEAHLSIASLRALHAEA
                     RFSELLRRLLAVQIASGITLAIRSYGTERRLPVEIESNLLRIIQECVANTVRHAKAAL
                     VQVELHYAPELLSVTVSDDGTGFDPDAAPGTEEGHYGLAGMRERSARIGADFQLTSAP
                     AGTCIRVRVPLPRSRSVAWGYTLSLFRRTLRRLGGLRLAAFGRSEQMDAKDNPRFDLR
                     "
     gene            complement(21740..22663)
                     /locus_tag="VHE33_21230"
     CDS             complement(21740..22663)
                     /locus_tag="VHE33_21230"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HVU00036.1"
                     /translation="MLRPSLAENSVPTIAFIAQTTGNELWEAAHAGARKAALRDGFRI
                     YWNAPTRSDDVERQIELIESAIRREDVGLVVAPTQYLALISPLRLAIARHIPVVVAGS
                     SVPIPAGNGLVFVLNDDQVMGRMAARRVGMLLHGSGNVALLGNDPNSTGNWIRSRAFE
                     ETLVSEFPAVSIRERDSSSPTFEEAAENAEKILLRNSQLAAIVSLTSTDSEGALTALR
                     LLHRTKDVYLIGCDQELDLMEGIREGQMDSVIAENSFMIGQRAEEAIAALRHRRSVQA
                     TVLIPPLLITRDNIDRPDIQQILSTDWRGAP"
     gene            22962..25400
                     /locus_tag="VHE33_21235"
     CDS             22962..25400
                     /locus_tag="VHE33_21235"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HVU00037.1"
                     /translation="MSSEGLNRREFSALLAGAAISLKGGSGTEPMGGSVPLQNDDQTD
                     HAFPAGNYTPFGYLDNPGHSAVMNRSGVIRSVPPLGFGWWARRMPWPYGLGALREVNY
                     LSLLQIAAAIDNDLFLTPEDFDKQGATLSSDHHTKNLFRYNWEHKGVRFRISWVLASP
                     DELVARFDLHNASQEMRHVTLHATNIYGFPEEQWWGSDGACTAIKGGRAVSKIWAYGD
                     VFTLSADREPHCAKATMDEAEWERWIRDNDASSNSGSSGKFPGATRALLSYDLQIGGG
                     RKEVLTLVLTRGVDQVSSERHSRSAIAEASTTLQRRLQDDDRFYAKAALLSGDWPEQW
                     KNGWIYDLETLRMTMRPPLGIYKHAWDGMQIFTPRAVLGESMLDSMALSYADMNLAKE
                     VVLGTFADAPAANVPCSREDGSMNMICADGSEVGTAPTWGMPFRVIRSMYVRSGDREW
                     LAALYPHARNFLNWWLANRTDGSGWFHCKCSWESGQDASKRFLVAEENPGATADFVRT
                     VDVEAAMAEAFQAMAQLCTDAGFRQDQARWTELAQKRIASTQAMFVDGWFRDFDGRNN
                     QPIILKDYYDVMMLYPLAAGITTPDQAHALTPRLGYFADNPRFWLEWPSFMFPYTEAA
                     WNAGQRKLAGEVVARTADRAYRRTDARKTKSIAPFESTLPREYQYRIPGIANEFWAVD
                     EDNPGGCENYGWGATLPTLILRNVVGFREFEDPARNALLLAPALPAALMKAGANYEVK
                     NVSLRSVTMSLTFSVVDAANINIRVQVSGKLKSIAATESAGKRLATADTRSGEAVLEF
                     RAVNGDVYNLSFQT"
     gene            complement(25429..27348)
                     /locus_tag="VHE33_21240"
     CDS             complement(25429..27348)
                     /locus_tag="VHE33_21240"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HVU00038.1"
                     /translation="MALTPLKLGSIRPEGWLREQLLLQANGLGGHLDEVWPDVGPNSG
                     WIGGTGESWERGPYFVDGLVPLAWQLEDQTLQSKALRFIDWTLGHAQSSGFFGPGSNN
                     DWWPRMVMLKALCQYEEASGDDRVIPVMERYFDYCLRELPGRPLRDWGRYRWQDQALS
                     VIWLYTRNGNPKLMQLARLLAEQGYDWRAQFTDFRYTFRCTPEYLGLNTKVKFPDRSM
                     QTHGVNNAMALKVSPVHYLLDGNPEDKTGILRQFAALDRWHGLPNGMFSADEHFAGPN
                     PVQGIELCAVVEAMFSLEQALAITGIPELGDRLERIAFNALPGTFTDDMWAHQYNQEP
                     NQVEVSLHRRPWTTDGPESNIYGLAPNFGCCTANFHQGWPKFTASLWMQTPDAGIAAV
                     AYAPSTVRTRIGPVPVTIREETNYPFREEIRFHVDPASPHTFPLKMRIPGWAHGAQVR
                     VNQEPGRPVEPGSFAEIRREWTRGDGVTLTLPMPARTSRWYRDSVAVERGPVVYSLPI
                     GASWLKLVDRGQASDWQAYPASAWNYALALNPDQPDAALTVHEAPMESQVFTLAAAPV
                     RIEVPAFPVPGWREEDGVADSMPASPVPVDNSKSITRISLVPYAAAKLRITAFPHGIP
                     GAHRQADDEAPEKKS"
     gene            27956..31174
                     /locus_tag="VHE33_21245"
     CDS             27956..31174
                     /locus_tag="VHE33_21245"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HVU00039.1"
                     /translation="MNKVHRVITALFSFAVLSVLLLAALPAHGQSATGTIEGYLSDSS
                     EAVIPGVTLLATNVATNQQYKANSDPSGHFSFLELPVGTYEVKVSLPSFKEFTRTGII
                     VYANSHNRVDIVLQVGSTADTVHVSGLPPQVNTTSPTVDSTITEKQITDLPLNGRDAG
                     SLVTLAPGTNNYSNVNWWGFPTTFITSNGSYFQNRGTDWLLDGGLFTWTYVNSGLQLP
                     NPDALEEFTYSSSNRASEFGRNDSATVNAVIKSGTNQFHGNVWEFNRNAGLNARSYQQ
                     SNVNPKLVQNQFGGTIGGPVLKNRAFFFGSYEGYRQTGSAFSFAALVPTEAERQGNFA
                     GVTPAPVDPSTGQPYPGGQVPVDPVIAKFLQYVPKPNLGTNGWQGNSPNNSNYDEYVA
                     RGDYDLTQRQRLTGSFYRLNNHIVSNQGSTIFLDPSATEYITSGKQYQINASHTYTFS
                     PTRLNTARYVFLDVGASRGWLNSNFTLQSLGSQFSSAFAQPPDIIVQNYFNFDATNDG
                     TVYTHNQELEDTFQWIKGSHQLTMGGQGIYYHDNQFTSPGPWIYFYGTATGNALADFI
                     AGNVGSFQYSVPNLTADVTVHWLFSGFVNDNWKVTRKLSITAGLRWEDLTGVTNPQDY
                     RTTFKPNVQSQVFPNYLPGWLFKNTITGKTDPGWSNAGYQAPQEVDPRIGFAYDVFGN
                     GNTAVRGGIGIYGGEVETIGWQNGSPFANPSPSCFTGNQTFVPISNPYVNTCDPITAA
                     KGWTGPPADYSAPIPYAGGGLDPRTKKPYAFNYSLGVQHQISQSMYVEASYVGSLVRK
                     VWFNYDYFGGAQYAPGATESTQSLADRYPYLTGKVSGVFVNGTPDNASYHGLLIQFNR
                     RMRSGLQFTTSYTWSKALDANHWPVQNWAVPRMRWGISDSDFASNLVGSFVYEPPVHL
                     DNHFAQAILSGWELTGLVQFQDGQPLTVLTGTDNLVNSYYGSRPNQVSDPTLSAHRSR
                     PAEMAKWFDTSAFADPGIGVAGNIGVDTLRGPGRKNINASLLRSIPITEHARFEFHFD
                     TFNTLNWVNLGNPNSTMNSPNFGKITSAGAMRQLQLGGKINF"
     gene            31289..>31974
                     /locus_tag="VHE33_21250"
     CDS             31289..>31974
                     /locus_tag="VHE33_21250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00040.1"
                     /translation="MLFRSLSAVALSIVLWTTSFAQTNDTSPFDRLSRPDKTELTDAF
                     SNVAATGGEFGLPWMWWWHRINAGWGTVRPEVARLVVYDERSHQTMWLQGGERTWTAD
                     HLHQSAKLNGLTLSEDKSVVGDSVADVLRITNGSAEARSLKLYFLGHPEAHSPGNPDS
                     RGLSIAFDPSSNSIRLTEEKRYGQHYLPGVIEIDQRIGSSLPISGWAIGTFDGDVEKF
                     LGRDLGMGAF"
BASE COUNT         6904 a         9102 c         9666 g         6302 t
ORIGIN      
        1 caagggagcc ttcgtgaatg ggggagaatc gagccgggtc gccaatctca cccggcttat
       61 gtcgggctgg tccaatgggg tcgtcgatct ggacaatgac ggctggaaag acctggtggt
      121 ggcgcgcagc aatgtgatgg acaacatcga gcaaatctcc cggcatttcc gctatgccga
      181 accgaactcg gtctttcgca atctgggtaa cgggcagttc gcagatgtga gcgcaacagc
      241 gggagccgat tttatccgtc cggcaccgca ccggggcctt gcttgcggag acctggacaa
      301 cgatgggcgt gtggatctcg tgatcacggc gctgggcggg ccggtcagtg tacttcgcaa
      361 tatgacacaa acacgcaatc actggattct gttcaagctg gtcgggagca aaagcaatcg
      421 catggggatt ggcgcgcaga tccgcgtgac gactgacgat ggcaggaaac tttacaatga
      481 ggcaacaacc agcaccggtt atgcggcatc cagcgatccg cgtgttcact ttggtttggg
      541 cgactcgcgc ctgcttcggg aaattgagat tcgctggcca tcgggcaggc gccagttact
      601 gcgcgatgta agtgccgatc gcatcatggt agtcaccgag ccagaaggtg gaagttagcc
      661 aaagtggcca cacgagggtc tattcgattg agcgcagttc catcacgctg gtggctcgtg
      721 agctgcgttc ctgtgctgtt cgtcgccgcg caggcagcgg ggcagctgcc gccttcctgc
      781 aaacctccgg cgtcggctgc ccgcccgccc gcaggtacct cccaggcgaa ggtctacgac
      841 gcagtcggtg tttggttcgc ccaaaagggt gacttgaaat gtgccgttgc ggcctttgac
      901 caggcgctcc ggatcgagcc ccactcagcc gaaggccatt tcgatctggg gcttgtccgg
      961 cagcgacagc aacagaatga cgctgcggtt gaggagttca ggctggctct gcaatacgat
     1021 cccgccctgc tgcaggcgca ttgcgctctc ggctcggtat tgtccgaccc ggctgctgca
     1081 gaagccgaat ttcgcagggc actggccgtc aaccctcagc tggtttgtgc gctggacggt
     1141 cttgctcagg ttctgctgaa cggaggacga tatgacgccg ccctggacta ctggcggcag
     1201 gccactcaga ttcagcccaa tgctcccgat ctgcagctgt ctgtggcgac tgcgacttac
     1261 aaagttgcca aggcgaggca ggctgatggg ttgcccgacc tggatggctc cacggtcgcg
     1321 aatgcgatcg gtcttttaac cactctgctg agcaagcatc cgggcatgac cgcggcccac
     1381 tttaccctgg gcaacatcta tgccaacgaa cagcgttttc gcgaagctgc tgacgaatat
     1441 cgtgcggtag tcggccagga cccgaccgat gccgtagccc tggctgccga ggtgaaggcc
     1501 ctggttgact caaccgcgta taccgatgca ctggcgccgg cccgtgctta cgtgcatcag
     1561 aagccggatg attcctcggg tcatgttctg ctgggcatgg tctaccgagg actgggtgaa
     1621 tatgcggagg cagagccgga actggagctt ggggcggtaa aggatccggg cgattttgag
     1681 gctcattatc agctcgggtt cgttctggct aagctcggaa aacctgatca ggctttgcat
     1741 cagttgcggc aagctgtcgc gttgaatccg ggggacaagt cggcgcagtt tcaacttgcg
     1801 gcagttttgc gcgctcttgg tcagaaccag gaggcagaga agatcgtcga gcagttccgg
     1861 aaaacaaccg acacggattt ccgcaacagc cagctgacct ccgatggcat taaggcaaat
     1921 gacctgctgc aggcgggcaa gccttcggaa gccgcagaga tctatcgcca gatgctcaaa
     1981 gagaagccgg acagcgcatg gacgacttac aacctggcgc tggcactgga agcgatgcag
     2041 gacacgaagg gtgcggaagc cgcgctgctg aaggggatcg agatcgaccc gaaggcggcc
     2101 aggattcggg ccgaactagg cgcactgaag ttgacggacg gcgatacggt atcggcccag
     2161 cagtggctcc agtccgcgct cgaccttgag cctcagcttg ccgaggcacg tggcaacctg
     2221 gcaagaattt acgccaagaa gggcgacttc gcgaccgccg ggaaactcct tcatcaggcg
     2281 cttgaagacg atccacaata caaggaaggt catttacagc tcggtctgct gttggcgcat
     2341 cagcggaaaa tacccgaagc cgagcaggaa ctcgatagag cggtcgcgct tgcccccagg
     2401 gatcccaata tcctgtccac ggccggtaag gcgagggaag agatgggcaa gatgagcgaa
     2461 agcatcgcac tgcttcgaaa ggtggtggcg ctgaggcccg atctcgcggc tgcgcatctg
     2521 gatctggcgg tcgcgctcgc cgacagttac gatctgccgg gcgccctgac ggaaactaac
     2581 gaagcagtcc gcctggctcc gcagtcgggt gtcgtccatt tctatcgcgg tcgggttctt
     2641 tacgacctgg gccgcacggc ggaagcccag actgattttg aaactgctcg ccagatcgtt
     2701 ccgcaaatgc ccgagccgct ctactatctt gccctcattg acaagcagga gggcaggttc
     2761 tcgcttgctt cggggttgct ggaagagacg gtccagcggc agccccgcaa tgtgatggcg
     2821 tggtatttgc tggggcagtg ctacgagcag caggcggaga ccgacaaggc tgtagcagcg
     2881 tggcggcagg ccatcgctct tgattcgaac ttcagccagg cactctttgg cctggcgcgc
     2941 gcgctgcggt cgtccgatcg cggcgagtcc gataagttta tggcgcggta tgtcgagatt
     3001 cagaaacagc ggcgtatcct ggacagcgca gggaccctgg ctaacaatgg cgttgtcgcg
     3061 gcctccgcac acgactggcc cgaagccaca agccaattga aggccgcgat cgctgaatgt
     3121 ggcgactgtg cggtcaaagg cgacctccac aagaagcttg gcctgatcga ttgccaggcc
     3181 ggcgatctca acaatggtga aaaggaactg ctgaccgcca aaggtctcaa accaacggat
     3241 ccggaaattc agcgcgctct gcagctaatc gcgcaagcga gaaaccagca ctcagcctcg
     3301 ggaaaggcgt attagtgcgc ttagctcgca attttattcc tttggccgtc gcccttgcat
     3361 cgatcgcgcc aatgctgatc ggagtgaaga tgctttcaca gatggtttcc ggaaatgcca
     3421 ccgatcagcc ggtacgccct cttccgcctg gcatgaaggc gcccctcgtc gacttccgcg
     3481 acatcgcagc gcaggccggt ctcaccgcca ggatcatctc aggcgatctc gatcaaacct
     3541 atatcgtaga aaataccggt accggtgttg ctatcttcga ctatgacaat gatggcctcc
     3601 cggatatctt tctcgtgcag ggagaccggc tgcacaattc gggtccgccg ttgacgccgc
     3661 acctctatca caaccggggt ggcctgcact tcgaggatgt caccgccaag gcgggcatcg
     3721 gccaccttgg ctggggccag ggagtctgcg caggcgacgc ggacaacgac ggccacgtcg
     3781 atcttttcct gacgcagtgg gggcacaacg tcttcctgca caatctcggc aacggcacct
     3841 tccgggacga gacccgggag cgtggccttt accgaccaga gtcacgatgg agcacgggat
     3901 gcgcgttcat cgattatgac cgtgacggct acctcgatct ggcggtggcc aactacgtca
     3961 acttcaaagc gcaggactcg ccgccgccgc gtacccggag cggctgtaac tggaagggca
     4021 tgccagttcc ctgtggtccg cgaggactca agggtgagac gatgaccctc tatcacaacg
     4081 atggacacgg gcatttcgtc gatgtcacga aacaggccgg cattgagacc ctgcccgagt
     4141 attatggatt cactgtcctg accggcgact tcgataacga tggctggccc gatatcttta
     4201 ttacctgtga ttccactccc agcctgtatt ttcacaacaa acataacggg acatttgaag
     4261 aggaaggtct ggccagcggg ctcgcagtga acgaggacgg ccgcgagcaa gctggtatgg
     4321 gcgcctcggc cgccgactac gacggcgacg gacggctgga catcttcaaa accaatttct
     4381 ccaacgacac gaacaccttg tatcgcaatc ttggaagtaa taacttcgac gacgttacca
     4441 gtgccgccgg tctggcggtg catacccagt atgtgaaatg gggcacggca tttatcgact
     4501 tcgacaatga tggatggccg gacctgttca tcgccgacgg tcacgtgtac ccgttcgtgg
     4561 agaagtacaa cctgggagaa gaattcaaac agccccgcca gttgttctgg aatcgcggcg
     4621 acggccagtt cttcgatatg tccttcacgg gtggttccgg cattacggca aagcatgcgt
     4681 ctcgcggcat tgccgtgggc gatctcgata atgacggatc tcaggagatt gttaccgtca
     4741 atctcttcga gcccccctcc ttgctgaaga acttcgcgcc ccgcggcaac gccctgctgg
     4801 tccgcgccgt taccgcctct ggtcgcgacg ccgttggtgc gcggttgaca ctggcgatgg
     4861 gggggcgaaa agagatcgac gaagttcgca gcggcggata ccacatttct cagggagact
     4921 tccgcgtgca cttcggcatg ggacacgcca cgaaggcgga tctgacgatc cgctggcctg
     4981 acggccccgt aagcaacgtt gaaactatcc caggagtcga tgccaatcag tggattgtgg
     5041 tccgcgaagg gcgcggcatc gtcgaacgcc atcccttcac gcacgacagt ccgtagcggg
     5101 agttcagctt ttctaactga ctgcttccat tttcatagat tgctgataaa gttcaagtca
     5161 caaaaaagcg agggttcaca atgaatttgt cgagacgagc ttttgccaga atttgcgcgc
     5221 tcaccgcatc cggacgtgct cttacaaggg cattgccggg agtggcccag gtgcagggtg
     5281 aacacttcaa gacaacgacg gcacatccgg tggcctccat tcaggacatt gagacgccgg
     5341 aacagaaaaa cgcccggatg gagtggtttc gcgaggcgcg cttcggcatg ttcatccact
     5401 ggggtcttta ttcgatcctc gccgggcgtt ggaatggcaa ggaggttccc gggatcggcg
     5461 agtggatcat gaaccgggct tccatcccgg tggcacagta caaggccctc gcggcgcgct
     5521 tcaaccccac gcagttcagc gcggctggaa ttgtgggact ggcgaaatcc gccggcatga
     5581 agtacatcgt gatcacgtcg aaacaccatg acggcttcgc gatgttcgat tcgaaggcga
     5641 accctttcaa tattgttcag gcaaccccct tcaaacgcga tccactccgg gaactcgccg
     5701 ccgaatgccg caggcagggt ctgaaactcg gcttctatta ctcacaggat caggactgga
     5761 cggcgccagg aggtgcagcc tataagacgg gcgatcacca accgcccact tatcattggg
     5821 accctgccca gaacggcagc tttgccagtt accttgaaac caaggctatc ccgcagatcc
     5881 gtgagctgct gacgaactac ggtgaattcc cggcaatcgt gtggttcgat acgccgacaa
     5941 ccgacatgac gccggaactg gccggcaaaa tcgtcactgt cctgaatgag catccgaaac
     6001 tgatctggaa caatcgtctt ggcggtggct atcagggaga taccgaaact ccagaacagt
     6061 acattcctgc tcgcggctat cccggtcggg actgggagtc gtgtatgacg atgaacgaca
     6121 cctggggata caaagtcgac gacaccaact tcaaaagcac ggaaacgctg gtgcgcaacc
     6181 tgatcgatat cgcgagtaag ggtggaaatt atctcctcaa catcggcccg gaggcaaccg
     6241 gcgaggttcc acaacccgaa gtggatcgtc tccacgaggt ggggagatgg ctgggggtca
     6301 atggcgaagc gatctacgcc acccgcccca cactttttgg agacgaagcc ggttcattct
     6361 cgacgacaga aaaggatgag gatggaaaag cgaagttcat tccggcctgg aagtggaggt
     6421 cgacgaccaa ggcagacagg atttatatcc atctcttcga gtggccggga accactttcc
     6481 agcttactaa ggtgccgcgc caggtcaccg aagcttacct tcttgcggat tcgtcgcatc
     6541 gttcgctgaa ggtgactcga actgcagacg gcgtcgaagt agctcttccc gcggaggcgc
     6601 ctgatccgat tgcgagcgtg ctggtattaa agacggcgta aagggcttga gtctgacgat
     6661 tccgggaagt ttggaagatt cagctcgact ccagaatcat gttggcgaat tgaatcgtgt
     6721 ctcccgtgcg gatgagtccg atcacctggg gaacccgctt cttgaactca acgtgaggct
     6781 caaactcgac ctcaagtccc gacatggcct cggtaaaact acttacgagt gcgggagaat
     6841 tgcgcagccg gaattcttcc gccatgacaa tgtgcccaat cgtgaagttc atccgtatgg
     6901 ctcgcaggac atcgagcacg cggggtatgt catcgatcag ggacagatcc acggtctcaa
     6961 tcgcgggcca atagggaaat ccacggtcgg tgatcaggag agtgttggta tggcgaacac
     7021 gactgagcag ggaattgatt tgcggattga ggattccggt gcggaccatg cttatgcctc
     7081 tcttgccctt agtgcagcag ggtctgattt gaccagccgc atgtcagcaa aggaatagaa
     7141 cgcgatgaac acataggcga gagccgggac gacgtaagcg gcggccaggc ctccaaactt
     7201 tatagaaagc agacccatca ttggggtcag cacggcgccg ccaatgatcg ccatcacgat
     7261 cagagagccg cccagcttgg cggtctcgcc gagtccgcga atgccctggg caaagatcgt
     7321 ggggaacatc agcgacataa aaaagctcgt gagcaggagg gctatcattc ccgtccaccc
     7381 cggaaacaaa acccccattg cggccagcac tgtgttgatc accgcataaa tagccatcag
     7441 gcggctgggc tgcacgaaac gcatcagcca ggcagaaaag aaacggcctg cgccgaacag
     7501 tacaaggcta ccggtcagga aaaagccagc agacttctcc ggctcgccgg tgtaggcctg
     7561 aatgtacggg atcagataac tccaggtgcc cacctgcgcg ccgacatagg caaattgcgc
     7621 cacaacggcc ataacgaaat gcgggcggcg cattaagtct gcggcacggt gggaaccagg
     7681 gccgcgatcc tcctcagatg caagcgaggc agggaagcgg acggaggcga tcacgaccac
     7741 catcgtcaaa gtgaccgcag ccagtaccag gtaaggagcg atcacccgca gtgtttccga
     7801 gtgcagatag gcctggtaag catggcggct ctgtaactcg gccatttgcc cgggagtcaa
     7861 ctccacaccc gaaaaaatga agaccgttcc gatcagcacg ccggtgatcg agcccagtgg
     7921 attaaaagcc tgcgccaggt tcaggcggcg tgcagcggtg cgcggatcgc ccaattgcgc
     7981 gataaacggg ttggacgcgg tctcaaggaa cgacagtcca ctggcaataa tgaacaacgc
     8041 aaccagaaag aaggagtagc ggcccacctc ggcggcgggc cagaacagga gcgagcccag
     8101 actgaagaga aacagcccga taagaaatcc cgacttatag ccgaggcgcc gcatcaccag
     8161 tgccgcaggc atagcgagaa cgaaatagcc caggtaaaac gcggactgca ccagccctgc
     8221 ctgaaatctg gtaatcacga atgacttcat gaattgccgg atcaggacat cgttcaggtt
     8281 gttaggaatg gcccagagaa agaacagggt ggtgatcaag gcaaaaacga ccttgtattc
     8341 cctgggtacc agcggaacat cgccgctggt cgagtgcgcg gagttgtcga ggggcatgga
     8401 ggagtgagtc atccgatcgc ctgagatggc atctcctgat cgagggtgat catgatcttg
     8461 gtaaagcgtg caggatcgtc gctccatgcg cgcagcaact caggagcccg atccagggga
     8521 gcaatcgtac tgatcgccgc atcgacgggg aagcggtgct gctcaagcat ctgaatcacc
     8581 tgatgaaagt cctccggcaa cgcgttgcgc gcgcccagta tattcaattc cttctgcaca
     8641 aacaggcgag tctcgtagct caccggttct ttcgcgtaac caatgtaaac aacccttccc
     8701 gtgaaggcga ccagctcgac cgcggcgcgg aaagtctggg gaaggcctac ggcctcgatc
     8761 accacatcag gtccatctcc gccggtcagc tctgccaaag cctcctttgc atcgtgcttc
     8821 tctgtgtgga tcgtgtgcac tgcgcctgcc ttgcgcgcaa tttccagctt ggcgtcgtcc
     8881 acgtccatgg ctaccgtagt tgcgccgcgg aaagccgatg cggccacggc gccgagcccc
     8941 acccccccgc atccgaaaat cgctacgacg tcctgcggtt tcacttctgc ccgcgcaacg
     9001 gcgtggcagc ccacagttaa aggctcgacc aggcagagct catcgagact cagatcggcg
     9061 acaaaaagct tgttcactgg aacggcgaga tactccccca ttgcgccatc gcgctgcacg
     9121 cccagcgtct gattgtccct gcacgcgttg ggccgcccat tgcggcatgc cggacacagt
     9181 ccgcaactct tgtaagggga gagggtcacg tccataccga ggctgagatg cgacgggtgc
     9241 tcaggctcca gctcgacgat cgtcgcagcg atttcatgtc ccggaatccg cgggaacgac
     9301 accagaggat tccttcccct gtacgaattc agatcgctcc cgcacatgcc cacacgccgc
     9361 acgcgcagca gcgcctctcc tggacctcgc accgggtcag gcacgttctc cacccgaaca
     9421 tcccccggcg cgtgcagcac cgctgccttc acaggagatc tcctttggag gatcgagtcg
     9481 tcattggttt tcttcgcgcc ccgaaggcca gaagcgattg aataccggag cgaagatggc
     9541 gcgcaccttg cgtaccagct gaggatccgc gggggcttcg aaagcgcgga gactcctgcg
     9601 gacctgctcg cgcgtggcca tcccgaccag ggtcgtggcc actctcggat aatcgaaaca
     9661 gaagcggaga gccagatcgg cgatatcgta ttcacgatca tggcaaaact gagcagcacg
     9721 ccgggccgcc tcacgcatct cactcggcgc cggatgccag tccggcgggc ccgcttcggt
     9781 cagcattccc atgtgcagag gcgaagcatt gatcaggcca attccctgat tctccgccag
     9841 gggcgccaga acagcatcca tatcgtctgc gagcaggttg tagtggcaat agctgagaat
     9901 cgcgtcgaca ggactggttc tggcgatgtg gatgagagcc cggagaggaa aacctgtaac
     9961 tccgatatac cgggttttcc ccatttgctg cagccggcgc atcgccggaa ttgtctcttc
    10021 cacgatctgt gtcagatcgg caaattcgac atcgtgagcc aacaagaggt cgacgtagtc
    10081 ggtgcgcagg cggcgcagag attcatcgat gctggcgaat atgcgagggg ctgaaaaatc
    10141 gaactcgttc gcgccgtaac gtccacactt ggtggcaaga atgactttgt ttcgccgccc
    10201 ttcgagtgct gtcccgaggc gatgttccgc cagagacttg ccgtaatagg gagatacatc
    10261 gaagaagttg attccctcgt cgatggcaag atggacagcg cgttttgctt cttcaggagc
    10321 cgtaagtcca taaacgccgc caagcggggc agcgccaaac gccaccaccg agacttcgag
    10381 atccgttttg cccagcgcac gatactgcat ctggctggca cgtttcgaag cccagaccgt
    10441 tgtcgacgat cgaagcacct ttcctccgga ctaaccgagg gtcactttga gggcggctgc
    10501 ataatcgact gccggccggt actgcctcgg cagttcaaca cgcaaagcct cgcccgtctg
    10561 cttccagttg aggcgttcgc ccgttccgat cagctcgacc tgcgttactt tgcctggctg
    10621 cgttgaagcg gagaggccga gagactgcac gacgatcggc tccgccggcc atccgagaac
    10681 aatcgcatac acgacgttgt tggccttgtt ccgggtgaag cgaatgtctt tctggcccag
    10741 cttgctgatg ctgttgccct ggaaggagcc cgctttgacg ctgttgggcc cttcgccgta
    10801 gaccttccat ggccgggttt cgtagatggc ctcgccgttg acctgcatcc acgccgttat
    10861 accgtcgagg actgcgatct ccttgctgtc gatggtgcca tccggtctgc ctggaatatt
    10921 gaggatgaag gttccgttct tgctgacggt atcaatcatc cagtggatgg catcgcgcgg
    10981 cggcaaataa ccgccgaatt cgccgggttt ctcgtacaaa ctccggttgt aatgccactc
    11041 gccgatgcac gtttccgact gccatggata tttctggatg ccgctggtca gtccgcgctc
    11101 gaagtcagca accaccgatt tgaccaggtg gtcggggact tccttaatat tgaggaccgc
    11161 ttccatcctg ccgctgttgg tgcgcaggct gtggttataa aagtaagcgc caatattcat
    11221 gccgccccag ccgagcggca gcagggaatt gtcgaaatag agcagatcgg ggttgtgctg
    11281 atcgatgagg tcgcgggtgc gatcgtaaaa gttcctgacg taggagacat ccggcagcgc
    11341 gtcgaaggga tgcttgacac cgtagagccg ctgcggatca agaccctgcc accactcgtc
    11401 cataccttcg cctgcggtga gatcgccatc gtagggaaca ccggccaggg gaccggttgc
    11461 gtcggcgccg tgcgacggct gaaaccacca ccagttgcga gcctgatgca cggtgacgcc
    11521 gaagcggagg ccctgtctgc gggcagcagc cgcccaggtg ccaaccacat cgcgatgcgg
    11581 gccgagcgcc gcggagttcc acggctggtg ctttgagtcc caggcatcga atccgtcgtg
    11641 gtgattcgcg agcgagataa agattcttgc gccggccttc ttgtatctcg caatcagctc
    11701 atccggatcc cagttcagca gagtccactg tgcgcagagg tctttgtatc caaactgcga
    11761 gggcggcccg tagtgctcga gctgatattt gtactggtgc tggtcctgga ggtacatatt
    11821 gcgtgcatac cagtcgcctg cttccggcac acactggggg ctccagtgag cccagatccc
    11881 gaacttggcg tcctggtacc attccggagc gtcgtactcc agcagcgaat cccatgttgg
    11941 cttgaagggg cctcgtccgg agaggccggc gatgggagat tccatcggaa cgccgtccca
    12001 ggatccgccg ggaacgggca gcggctcggg gctgccccag gccgtggctt tccagtcggc
    12061 gttgtccagt tttgccggga cgattttgcc attgctcaat ttgtagaagg tgcgcttgtt
    12121 gggcggcagg gcgcagaagc gggcgtagtc ctgggagtat tcattgaagc cggcgggcac
    12181 ctccgaggag ctctccggcg ctgactgccc gatggctgga atggctttgg ctgcggcggc
    12241 gaccgccagg agtctgcaga aatcacgtcg ttgcacggcc ttgctcccag ttcggatgtg
    12301 cttgagtgcg catctccgaa cgaaggggcg atagtatcaa ccgtatctca gattgtcaac
    12361 gatacagtta tcgtttaaga cggctacact cgcggcagcg aggccggcga tcaggaatcg
    12421 tcgctggatg ggtctggagc gcgccggctg gaagcgcgaa ccacaaggcg cgagctgatg
    12481 tactccgtct tcggcttggt tggcgttttc ttacgggcga ttttgagtgc cagatcagct
    12541 gcgcgcttcc cgatggtttc actcccctga tccaccgaag acagaggcac gcgcagaaaa
    12601 tccgagtacg agaagttgcc acatcccacg acagccatgt cttctggaat ccgtaacccg
    12661 gcttccagga tggctcgcat cgcgcccaga gcggagggat cgttgaagca aaagatgcca
    12721 tcggggcgcg cttcggcagc aaggagttgc tttgctgctt catagccgcc tttttctccg
    12781 cgctgatctc ccgacgaacc gagggaaaca acgtggccag gcagtggatt cagattgtgg
    12841 gacgccaggg cacgggtgta gccctccaga cgcccgattg ctgtgctgac attggggccg
    12901 cggatgtgag cgatacgacg gcagccttgt tcgtacaggt gggaagtggc cagcataccg
    12961 accgacgcgt catccacgcc gacgaaattg gcgtcgagat catcaaaccg ccggtcaagg
    13021 agaatgtagg gcgttttctg ctcctcaatc aggcgaaagc attccacaga cgattgcgcg
    13081 gaggcgacga ggatgacatc cactcgcctg gccagcagct gtttgatctc ctcaatctcc
    13141 agctccgggt cctcgtcgga tgatgagatg agaaggctat agccgtgttt tcgggtctcg
    13201 gtcgagatag ccttggcaat ttccgcgaag aagggatgaa gcagatcggg aaccacgagt
    13261 cccaaagtcc atgtccggcc ggtcacgagg gagcgcgcgg cgaaattggg ctgatagttc
    13321 agctcccgca tgcgcttctg gacccgtttc cgggtcgcct ctccgatgtc gggatgattg
    13381 cgcaggacct tcgataccgt gacaacagaa agacccaggt cgtcggcgat gtcctttaag
    13441 cgggctgcca tgaattcagt ggctccaggc tggttccggg cttgcgcaaa gagtgcgctg
    13501 ggcgaacagc actaaaatcg cagaaagtca ccgtttactt taattacttt gggacagccc
    13561 gtcaattcag ctcagctatc tcatggcaaa ggattttcgg tgagggaggg gcatcgacga
    13621 caccacacgg cgtctgcgct caggaagcat gagttgccgc cgaaacgacg gccgaaagcg
    13681 tggcttacgt ggcgtagctg agcttcaaac cccaggcgaa ctggctgctt cggttcgaag
    13741 ggtcctgtaa ttcggtgggg atcgagatca caaagttgtc cgtcgcatcc ctgcgaaact
    13801 ccagcttctt tggataacca aacatctcca ctgaggaaac ggggcgtgca tccagcgatc
    13861 ggatcttcag actctcgccg ggccaatccg tgacgaagca atagaggatg cgtccgtcct
    13921 tgctgcaggc aaagcgaagg ttgttgccct cggcccaatc ggtgcctgca cgagggcgcg
    13981 tcccgtagat accctcgcca ttgatcctca gccattgtcc ggcctctttc agctgcgcga
    14041 tggcggcggg gtgaaagctg cccatgccgc tggggcccac cccaaccatg aagttgccac
    14101 cctttgcggt gacgtcgacc aggttatcaa tgatccactt cgttcccttg tacttcgaac
    14161 cgtcactctc ataggagaaa gatgttccaa gcgggtaaat cacgaaccac ggcattttcg
    14221 tggctgcctt cgttgcagga acgaatcgct caggcgtgta gtaatcccca tagttgccga
    14281 tgccgcgcgc gcgcagcatg acatcgggtt gtagttccct gagcttcagg attgtctcgc
    14341 gcatctgcgg ccagacggcc ggaccccaga agatgtcgag gcacatcata tcgatcgtgc
    14401 cgtagcgagt gagcaactcc gtaagctgcg cgcggtggcg ctccaccatc cgccgaactt
    14461 cgctcggagt cgggtccggc accgtgacca gacgaccgga cattcgtttt ttcatggaat
    14521 cccaaaattc ctgggtcgtg ccgtccgaag gcgtccagcc cgagagtctc ggcgaggacg
    14581 gaacctgcag cggatgccag ccatacgggc gaaagtcggc gtcgtaccaa tcggagtgcg
    14641 agaagtagag gtcgattttt atattgcgct tgtgggcgga atccgtaagt tcgcgaatca
    14701 catcgcgccg gaatggcgtt tccataatgc tgtatgccag gtcgcagtct tcgatgcggg
    14761 ggctcccggg tgctgtccag ttggtccggt ttcggacgcg ggtcttcgta tcgaacatcg
    14821 agaaaccttc gtgatgcttg ctggtgaagg caaacatctt catgccggat tcctcacaca
    14881 ggttcatcca tccatcggcg tcgaattccc gcggattcca cgatcgaaag gcctgattgt
    14941 attcctggcg tttgtcgaaa ggcagggtta gaaaaggcca tgattcacca ggcctgccgt
    15001 agattgaata gatcccccag tggattctca gtccgaactt catgtccctg aacgcttcga
    15061 cggcgcgatc cgaagcccag tgataccgat gcaccgggac ttcttcgatg taggatctgg
    15121 agcgctggta gtcggcgtcg gtcgggggca gacgatgctc gatcgggggc gcttcgtcct
    15181 gagcccaacc gccgagcgag gaggtggaac ggagagaagc ggctcctgcc gcaacaagaa
    15241 attcccggcg attcacgatt tctcctttgc aggcacggct aagccgtccc agccacatcg
    15301 cgggtgaatg ggttcagcga tggcgctgct tcaagcgaag acggcgattc gctcatccta
    15361 atatgaggtc gaaattcatc cgcagatcgg ccgtgccgca tggcctgaaa tcctgataag
    15421 cggcttacga gccaaggaac aatggcagca ggacgacgcg accgaggctc tattcaatct
    15481 tgttgagggc cggacagagc acctccgcgg tttggtgaat atttattttg tggcacgatg
    15541 cacggtcctg gcgatgatct ggctaagctg ccaggtcgcg aatcgcgtat tccggtgctg
    15601 ggccgacagc acggtgataa cctggttttg ctgagaaaat cggcccgcca atttgcccgc
    15661 aacagcagtc aaaaacccca gaacacgaat ctggcgtcac ttccgcccgc tcttcgatga
    15721 gctgcgatcc cgtaacaatt gtgattgttg gtttaagcca acgacctttg ggtgatttgg
    15781 ggttattggg ggaatcggct aagtggttgc gggggtggga tttgaaccac cgacctttgg
    15841 gttatgagcg tccaggcgct caagtatcta acagactttg cggcacttat ggctacccgg
    15901 aagtacggaa aagtatcaga tggaccacct taatgggagt tctgttcggg agcgcgtttt
    15961 gtgagaaccg cgcgatcatt tgatagatta tagataatgg tactgccgga cttcgcacga
    16021 ttgcgcattg ccaaatgggc ttcgcaaaaa ggtgacgtgg ccatggggtc tttcagaaga
    16081 acaagggctt ccctggcgac cttcctgctg gtgtccgccg ccctcgcgac cccgcaactc
    16141 gcggtgactc ttcatgctca atccaccgat gcagcacaac tcgattcggc cgatgccggc
    16201 ttgttccagc aggggcttga cgctcaacac aagcgggacc tcgctactgc agagaacgac
    16261 tatcgggagc tgttagcccg gagttcagga tttttgcctg cgcgattcaa ccttggtctg
    16321 gtgctcgacg ccgagggcag gacagcggaa gcgctggacg ccttcgaggc ggtcgtcgca
    16381 tcagatcccg gctttcccgc cgtacagatg tttgccggca ttgagaattt caggctgggt
    16441 cgattgccgg cggcacagac ggcgctggaa cttgcgactc ggcagtcgcc ggagaatttg
    16501 cagtgctggt tctggctcgc gcggacggat ttcgctgccg ggaagacgct ggagggcaaa
    16561 gcggcggtgg acaaagctct ggcgatttcg cccgaggagc cacgtgtcct ctatcttctt
    16621 gctcaatatc aaatatccgc gcaggatctg gccggcgccg agcgcaccct caccggcctg
    16681 gcaacgcggt acccgaaggt gcccgatttc cacgagtccc tgggcagcgt ctattacctc
    16741 gaagcccgac tcgacaaggc cgaagaggag tatcggaccc aattacagct ggatgcaaat
    16801 aatccccagg ccttttccat gctcggagtg atcctgctgg atcgcggtca ggcgaagcag
    16861 tcgattgagt atttggcaaa aggcctcgaa gcgaacccgc ggattgctta cctgcaacgc
    16921 aaaataggtc aggcgctgct ggagagcggc caggcgctgg aagccgtgga gcacctgagg
    16981 caggcgacgg cgctggatcc gcgcgatgca accgctcact ttcttctctg gaaggccgat
    17041 agctcgctca accggaagac ggaagccgcc gcagaactcg aggcgttccg aaaactgcaa
    17101 tcccagccac aaatgccggt gggactgccg ccgaccacgg cgcagggagc ggtgccgcat
    17161 tgagatcgaa aacaacaagg cgtgacttcc ttcgtgattt agcctggctt cctgcagcga
    17221 gcgtttggcc cggacgcgtg tgggcggatg ccggcgatcc tgcagcaaaa gcgaatccat
    17281 atcacgtgga gttcgacgat gtcaccgcag agagcggcat tcggtttgtg catgagcgcg
    17341 cggcatcgac cgagaagctg gttgtggaga cgatgggcgc gggctgcgcc tggctcgatt
    17401 acgaccagga cgggtttctc gacgcgtttt ttgtgaacag cggcgcgaca ccgcaatttc
    17461 atcctgcatc gatgccgcag cctgccctat acagaaacaa cggcgacgga actttcaccg
    17521 atgtcacggt caggagtgga attcgagtca acgacggttt ctatatcggg gcggcggtcg
    17581 gcgatttcaa taatgatgga tatccggaca tctatatgac cggatatggc aagtcagtgc
    17641 tctatcgaaa caacggcaac ggtaccttca ccgacataac ggctcgggca ggcgtggaga
    17701 atgccggccg atgggctacc gcagccgcct ggttcgacta tgaccacgat ggacggctcg
    17761 atcttctggt cacgaattac atcgactacg actggaatca cgatccgtac tgtggagcgc
    17821 ggcttcccgg ttatcgagtc tattgcgacc cgtttcattt ccgcgggacc tcgatgcggc
    17881 tgtatcacaa caatggcgat ggcacattta cggatcgcac ggaacaagcc gggctcctga
    17941 atccggaagg caagagcctc ggactggtca tcgcggactt cgacggcgac ggctgggatg
    18001 acatcctgat cggaaacgac ggcatgcgtt cgtttctcta cttcaacaat cgcaatggaa
    18061 ccttccggga tgcgacttat gaatcaggcg ccggattcgg gggaaacggc gaagtggagt
    18121 cgggcatggg catcgatgca gccgatgcga ccggcaaagg acggatggac ttcttcgtct
    18181 gtcatatgga caatcagccg aaccggttct acgtgaataa tggcgacggt acattcacag
    18241 accgcaccat gaataccgga tttggcttca gcggaatcag gaatacgagc tatgccgttc
    18301 gttttgtcga ttgggacaat gatggcaatc gtgacctgat cgtggtgaat gggtcgatgc
    18361 tcgataatat tcggctgtat catccggatt ccagctatgc ggaagcaaaa atgctttatc
    18421 ggaatacggg agagggtcag tttgtcgatg cctcaggcac gcaaccggca tcgtttggag
    18481 tcgcacgggt gggtcgtggt ttggccgtcg gcgattacga caacgatggc aacatcgact
    18541 tcctgcagag caacaacggg gagcaggcgc agctttatcg taatctcggc gccaggggca
    18601 atcactggat cacgttgaag ctgatcggga cgcgcagcaa tcgagatgca atcggggcgc
    18661 gcgtgcagct acgtgccgga gacttcttca gcttcgatca ggtgcgcagc ggcggcagct
    18721 actgctcctc gcacgacctt cgccttcact tcggcctggg agcacgagag aacgcggacg
    18781 aaatcgaaat ccgatggccc agcggcgtca ccgaccgcct gacgcatctc aaagcgaatc
    18841 agttctacgc ggttcgcgag ggggaaggag tcgtaccctc taagttcccc cggtttcgca
    18901 atcggcatta agcgattctt cagcgcggtt cgtcggcgat gatgatgccg cgctgcaggg
    18961 cggcactgac ggcctcggcg cggtcgctca cgtcgagctt ggcaaaaatg ctgatcgtat
    19021 gatttcttac ggtgttttcg ctgttcccca aaacgcgtgc gatctcgcgg ttggttagtc
    19081 cgctcaccag cagacggagt acttcatatt ctcgtgctgt gaggtcaggc cggccagaac
    19141 gctgacgaag aatcgcttcc atccatggag gtaccgatcg acggccggcg gcaactgcgt
    19201 gaatggcctc gagaagcggt tccctgccga cgtccttcaa aatgtatccg agcgcgccag
    19261 cttcaagggc ctgataaatc tcctcttcgc ttgtgtagct ggaaaggatc agggcccggg
    19321 tttccgggga ttcactggag acggtgcgca gggcttcaat gcctccgcca tcgggcatac
    19381 gcaggtcgag aagcagaacg tcggggcgaa gctggcggca tgcctcgatg gtgtggcgtc
    19441 cggttgccgc ttcgccgatg acatcgatgt cgggctctcc gctgagcagg ctcctgaggc
    19501 caagacggaa cagcaggtga tcatcgcaga tcaaaacgcg gattgtcttt tgcatccatt
    19561 tgctccgagc ggccaaaggc cgctaaccgg agaccgccga gtcgacgcag cgttctcctg
    19621 aatagagaca aggtatagcc ccacgccacg gagcgagaac gcggaagcgg aacgcgcacc
    19681 ctgatgcagg tgccggcggg ggccgatgtg agttgaaaat cggcgccgat gcgggccgac
    19741 cgctcgcgca taccggcgag cccgtaatgg ccctcctcag taccgggcgc agcatcagga
    19801 tcaaagccgg ttccatcgtc ggagacagtg acggacagaa gttccggagc atagtggagc
    19861 tccacctgaa ccagcgcagc tttggcgtgg cgaaccgtat tggcaacaca ctcctggata
    19921 atgcggagaa gattcgattc gatttcaacc gggagccgac gctctgtgcc ataactgcga
    19981 atggccaggg tgattccgct ggcaatctgc accgccagca ggcgccggag caattcagaa
    20041 aagcgcgctt cggcatgcag tgcacgcagg gaagcaatag aaagatgggc ttcaccgcgg
    20101 ctgcgttccg ccatctgcag agcgcgggtg accgattccg gagtgctgat ccgtccttcg
    20161 cgatcgatcg cgctttccaa ctggaaggcg attgcggcat agccctgcgc cagggtgtca
    20221 tgcacctcgc gggccatgcg caggcgctca tcgagcacag cccgcattcg ccagtgttcg
    20281 gcgcgagaat agacaaagag tcccagaagt gcaacgacgg gcaatgccat gcctgccgcg
    20341 aggaggttgc gcagagccca ccatgggggt ccggccagaa catggacgtc gctcgaagaa
    20401 cgaagcagga tggcaaatcc ggcggcgttg ccggtgaacg ccggatcgac gacgcagacg
    20461 ccccgtacgc ggaccctgct gtgaggcctg atgttacgaa gcggggaagg attggcctgg
    20521 gagtcaacga tggagacaaa gtgctggtaa ccgctgtgca gcaacaactc gatttgtccg
    20581 ccggcagaat cccggacacc atcgacttcc gcctcggttt cgatgaagcg ggcatcatag
    20641 aggccgacag cggcctgggt ggccgataca gagatgggag gggcgggact ccgctgccaa
    20701 aggacgtgac cggtggcctt gtggagcgcg gcgctgaaat gagaaggttc cacctctccg
    20761 gcaatctgca cttcgtcgcc aatcttgagg gatggcgcgt gcaggaattc gaccgtcgcg
    20821 ccgccagtcg catcctgcac gtacacacgc ggagaaatga gagtgacact gccgcggatg
    20881 gcgacggtga caggttgccc tgcagataaa ccgctcaact ggccaatggg cgccagtggc
    20941 gcggtggccg cctggtgaag attttcggtg aactgggagc tgatgcctgg ctggttcagg
    21001 cgcagagagt tgaagccagc ttcgctctgg agaaagcccg gtttcggttg tggcggagcc
    21061 tgagccagca gcggattgag ttcgccggag cccgcgcggc tgacgcgaat gttcttccac
    21121 aggccgccgg aagaataaga acgcaggccg atgcgacccg accgagcgca cgagggctcg
    21181 tacatagcga cccaggtgcg gtctccggga gtgccgctgg atgcgaccgc tgcgatcgaa
    21241 cagccgacgg cgacgatatg gaggtggtac cagtgggagg gaagaagccg ctcacgtgga
    21301 gaaacggcct ggtattcggc ccaaccgtga tcggcgcggc cgatggtgag gctgttgttg
    21361 ctgtcgcgca gaccgacgta atagccgctg taagagtcca ctccctcctc ttcatcgctg
    21421 gacctcacaa tgatgcccgc gtcgccatct tctccgagga ggctcacgtc agcgtcgacg
    21481 gcataatcgg accacgcggg agaaccggtg ataaatttcg caccgcgctc atcggagttg
    21541 tttcgaattc ctcccttgta cgggctccac gctccaccga aagcctgcca gccgtcaatg
    21601 tcacccgatg caaaactgtc ctgccacggg aggcgataac tccgattggg ccagagtgcc
    21661 agggcgccca caagagcagc gaagagcagc actgccgtga cgatgacacc cccgaaggag
    21721 cgaggcgcgc cgcgcagcct catggcgcgc ctcgccagtc ggtggagagt atttgctgga
    21781 tatcagggcg gtcaatattg tcgcgggtta tgaggagagg ggggatcaga accgttgcct
    21841 gaactgaacg gcgatgacga agcgcggcga tagcctcttc agcacgctgg ccgatcatga
    21901 aactgttttc cgcgatgacc gaatccatct gaccttcgcg gataccttcc attaggtcca
    21961 gctcctggtc acagccgata aggtagacat ctttcgtgcg gtgcagcagg cgaagggcgg
    22021 tcagagcgcc ctcgctgtcg gtgctggtga gtgagacgat cgcagcgagt tgtgaattgc
    22081 gcaggaggat tttctcggca ttctccgccg cctcttcgaa tgtcggtgag ctggaatcgc
    22141 gctcccggat cgagacagcc ggaaactcag acacgagggt ctcttcaaaa gctctggagc
    22201 gtatccagtt cccggtcgaa ttgggatcat tacccagcag ggcaacgttt ccagaaccat
    22261 gcagcagcat gccaacccga cgagcggcca tccgtcccat gacctggtcg tcattgagca
    22321 cgaaaacaag gccgttgcca gccggtatgg gaacggagct cccggcgacc acgaccggaa
    22381 tatgtctggc aatcgccagc cgcagagggg aaattagcgc cagatactgc gtcggagcaa
    22441 ctacgaggcc gacatcttcc cggcggattg cggattcgat gagctcgatt tggcgttcta
    22501 catcgtcact gcgcgtcgga gcattccagt aaatgcggaa accgtcgcgt aatgcggctt
    22561 tgcgggctcc ggcgtgcgcc gcctcccaca actcattgcc ggtggtctgg gcaataaacg
    22621 caatggtcgg gacggagttc tccgcaaggg aaggccgcag cacaaaccag gagccggcga
    22681 ggatgagacc tgcgactccc ggcgccagcc atgtccaatt gcgtctggat cggtgcgacg
    22741 gaggacgtga aacggacctg actttcatac tggacttcgc ccgatcagga taggttgcga
    22801 tcctcgcacc aatgtactgc ctttgaaact gcgctgcaca ccatccagat gcaggaggcg
    22861 aaacaggccc gtcaatagtc cggatagacc cctccgtgat ccctgcgggc aaacgtagaa
    22921 tgttcgcgcg cagagagcgc catttccgcc ggggggaagc gatgagttca gagggattga
    22981 atcggagaga gttttccgct ttgctggcgg gagccgccat cagcctgaaa ggcggaagcg
    23041 ggacggaacc catgggcggc agcgtgccgc tgcaaaatga cgaccagaca gatcacgcgt
    23101 ttccggccgg caattacact ccattcggct atctggataa tcccggccac agcgccgtta
    23161 tgaatcgcag cggagtcatc cgcagcgtgc cgcctttggg atttggctgg tgggcgcgac
    23221 ggatgccgtg gccttatggc cttggcgctc tgcgcgaggt gaattatctt tccctgctgc
    23281 agatcgctgc agccattgat aacgatctct ttctcacgcc ggaagatttc gacaagcagg
    23341 gggcgacgct ctcatcggac catcacacca aaaatctctt tcgatacaac tgggagcaca
    23401 agggcgttcg ttttcgcatc tcctgggtcc tggccagccc ggatgagctg gtggcgcgat
    23461 tcgatctgca caatgcgagc caggaaatgc ggcatgtaac gctgcatgca accaacatct
    23521 atggttttcc cgaagaacaa tggtgggggt cggacggcgc ctgcactgca atcaaaggag
    23581 gccgtgcggt ctcgaaaata tgggcatatg gagacgtctt cacgctctcc gccgatcgcg
    23641 agccgcattg tgcaaaggca acgatggacg aagccgaatg ggagcgctgg atccgggaca
    23701 atgatgccag cagcaactcc ggatcgtcgg ggaagtttcc aggtgcgacc cgtgcgctgc
    23761 tgagctacga cctgcaaatc ggcggcggcc ggaaggaagt tctcacactg gtgctgacgc
    23821 ggggtgtgga tcaggtcagc tccgaacgtc attccagaag cgccatcgcc gaagcgagca
    23881 ccactctcca gcgaaggctg caggacgatg accgattcta tgcaaaggct gcgctgctca
    23941 gcggcgactg gcccgagcaa tggaagaacg ggtggatcta cgatctggaa accctgcgca
    24001 tgacgatgcg gcctccgctg ggcatctaca aacacgcctg ggacgggatg cagatcttta
    24061 cgccccgcgc agtgctgggc gaatccatgc tggattcgat ggcgctgagc tatgcggaca
    24121 tgaatctcgc gaaggaagtg gtgcttggca catttgccga cgcgcctgcc gcgaatgtgc
    24181 catgttcccg agaagacggc agcatgaaca tgatctgcgc ggatggatcg gaagtcggaa
    24241 ccgctccgac ctggggcatg ccgttccggg tgatccggtc gatgtacgta cgcagcggag
    24301 accgtgagtg gctggcggcg ctgtacccgc acgctcgcaa ttttcttaac tggtggctgg
    24361 ccaaccgcac cgatggcagc ggctggttcc actgcaaatg cagctgggaa tcggggcagg
    24421 acgcatcgaa gcgctttctg gtcgccgagg aaaacccggg tgcgacggca gactttgtgc
    24481 gaaccgtgga tgtggaggcc gctatggctg aggcattcca ggcaatggcg caattgtgca
    24541 ccgatgcggg ttttcgacag gatcaggccc ggtggaccga acttgcgcag aagcggatcg
    24601 ccagcaccca ggcgatgttc gtggatgggt ggttccgcga tttcgacggt cgcaataatc
    24661 agcccatcat tctgaaggac tattacgacg tcatgatgct gtatccgctg gctgcgggga
    24721 tcaccacgcc cgatcaggcc cacgcgctta caccgcggct gggatatttt gcggacaatc
    24781 cgcgtttttg gctggagtgg ccgtcgttca tgtttccgta caccgaggcc gcatggaacg
    24841 cgggccagcg aaaactggca ggggaagtgg ttgcgaggac ggcggaccga gcttatcgca
    24901 ggacggatgc gcgcaaaact aaatcgatcg ctcctttcga atcgacgctg ccccgggaat
    24961 accagtatcg aattcccggg atcgcgaatg aattttgggc ggtcgatgag gacaacccag
    25021 gcggctgtga gaactatggc tggggagcga cgctgcccac gctcatcctg cgcaatgtcg
    25081 tcggatttcg ggagtttgaa gatccagcgc ggaacgcact gctgctggct ccagctttgc
    25141 ctgctgccct gatgaaagca ggagccaatt atgaggtaaa gaatgtcagt ctgcgttccg
    25201 ttaccatgag cctcaccttc agcgtggtgg acgcggcaaa tatcaacatc cgcgtgcagg
    25261 tatcgggaaa actaaagagc attgcagcga cggagtctgc gggcaagagg ctggccacgg
    25321 ccgatacgcg atcgggagag gcggtcctgg aattcagggc cgtcaacggg gacgtttaca
    25381 acctcagctt ccagacttag gcggaggaag acagcgcaaa ggtcccgttc aggacttttt
    25441 ctccggtgct tcgtcgtcgg cctgacgatg tgccccggga attccgtgag gaaatgcggt
    25501 gatccgcagc ttggccgccg catacggcac cagcgagatg cgggtgatgg attttgaatt
    25561 gtccacgggc acaggacttg ccggcatcga gtcggcaacc ccgtcttcct cgcgccagcc
    25621 ggggaccgga aatgcgggaa cttcgattcg cacaggagca gctgcgaggg taaacacctg
    25681 ggattccatg ggagcttcgt gaaccgtcag agctgcatcg ggctgatccg gattgagagc
    25741 gagggcatag ttccacgccg aagccggata ggcctgccag tcactggcct gaccgcgatc
    25801 cacaagcttg agccagcttg cgccgatcgg caatgaatag acgacagggc cacgctcgac
    25861 ggcaacagag tctcgatacc agcgggaggt ccgtgccggc atcggcaagg tcagcgtcac
    25921 tccatcgccc ctggtccact cacggcgaat ctctgcgaaa gagcccggct cgaccgggcg
    25981 tcccggctcc tgattcacgc ggacttgtgc cccgtgggcc cagccaggga tgcgcatctt
    26041 cagcgggaaa gtatgcggcg aggctggatc gacgtggaac cggatttctt cgcggaatgg
    26101 ataattcgtt tcttcgcgaa tggtgaccgg caccgggccg atgcgggtgc ggaccgtgga
    26161 cggtgcatag gccaccgccg caatgccggc gtcaggagtt tgcatccaca aactcgccgt
    26221 gaatttcggc cagccctggt gaaaattcgc ggtgcagcag ccgaagttcg gagccaagcc
    26281 gtagatgttt gactcgggac cgtcggtggt ccacgggcgc cggtgaaggc tgacttcaac
    26341 ctgattgggt tcctgattgt actgatgggc ccacatgtcg tcggtgaagg tgcctggcag
    26401 agcattgaaa gcgatccgct caaggcggtc acccaactcg ggaatgccgg tgatcgcgag
    26461 ggcctgttcc agagagaaca tcgcctcaac gacggcacac agttctattc cctggactgg
    26521 attgggtccg gcgaagtgct catcggccga gaacatcccg ttggggaggc catgccagcg
    26581 atccagcgca gcaaactgtc tgagtattcc agtcttgtcc tcagggttgc cgtcgagaag
    26641 gtagtgaacg ggcgaaacct tgagggccat agcgttgttc acgccgtggg tttgcatact
    26701 gcgatccgga aatttcacct tggtgttcag gccgagatac tccggcgtgc agcggaaggt
    26761 gtagcgaaaa tcagtgaact gcgctcgcca gtcatagccc tgctcggcaa gcaggcgggc
    26821 cagctgcatc agttttgggt tgccgttccg ggtgtagagc cagatgacgc tcagcgcctg
    26881 atcctgccac cggtagcggc cccagtcgcg gagcggccgg ccgggaagtt cgcgcagaca
    26941 ataatcgaaa taccgctcca tcaccggaat aacgcgatca tcgccactgg cctcctcata
    27001 ttggcagagc gccttcagca tgaccatgcg gggccaccag tcgttgttcg aacccgggcc
    27061 aaagaagccc gatgactgcg cgtggccgag ggtccagtca atgaatcgca gagccttgct
    27121 ctggagagtc tgatcctcaa gttgccaggc aaggggaacc aatccgtcga cgaaataagg
    27181 ccctctctcc caggactctc cggtaccgcc gatccaccca ctattggggc caacatccgg
    27241 ccacacttcg tccagatgtc ccccaagacc gttggcctga aggagcaact gctcccgaag
    27301 ccatccctcc ggccggatcg aacccagctt taaaggtgtc agtgccatgg atccgagcgg
    27361 agcgcgggag gatcctgtgg cagagactgg ccttttgtcg ttgggtgagg aggacaacgg
    27421 aagaagggta cgtgtagccg acaatgccga cgaggcgccg agcgcgaaca ggaactgccg
    27481 ccggtcgaat gggttattca tcaggccgaa atctctccaa atgacacacg gtaagcaagt
    27541 tgtcggtatg cggaaatagt ccaagtggtc tattccgcag gcctgcgaac gcgaataaga
    27601 aaaatgatga agaggggctt gacagggctt ccatctataa tctataaacg cgcgtgttga
    27661 tgatcacgtt gaaatgaatt cgcgcagttt ccgctctggg tctcgggcaa ggaagcgcaa
    27721 atgatccgag ttttaacaaa atggccaaag attatagcag gtcaaatcct ggggtcaata
    27781 gtttattcgc aactggattt ttatttttac aaccgccgaa tatgctggcg gcgcgaaccg
    27841 gggagttcct gtgggtgatg ctgccaggcg accgcgaagc aagaaaccga agactgagcc
    27901 tgggcacaca tggcacgggc ctgcagcaaa gagttttgaa agcgaggcga cgcaaatgaa
    27961 caaagttcac agagtaatta cagcattgtt ctccttcgcg gttctgagtg tgcttctgct
    28021 ggcggcgttg ccggcacacg gacagagcgc gaccggcacc attgaggggt atctcagcga
    28081 ctccagcgag gcggtgattc cgggagtgac cctgctggcg accaacgtcg caacaaacca
    28141 gcagtacaaa gccaattcgg atccttcggg gcacttcagt ttcctcgaac tgcctgtcgg
    28201 cacctacgag gtgaaggtca gccttccgag cttcaaagag ttcacgagaa cgggaatcat
    28261 cgtgtatgcg aacagccaca accgggtcga cattgtgctt caggttggct cgacggccga
    28321 taccgttcat gtcagtggac ttccgccgca ggtcaacacc acctcaccca ccgtcgattc
    28381 cacgattacg gaaaagcaga tcacggatct gccgctcaat gggcgcgatg ccggatcgct
    28441 ggtaactctt gcgcccggaa ccaacaatta ctcgaacgta aactggtggg gatttccaac
    28501 gacctttatt accagcaacg gatcctactt ccagaatcgc ggtacggact ggctcctcga
    28561 cgggggtttg tttacgtgga cctacgttaa ctccggcctg cagttgccga accccgacgc
    28621 gctggaggaa ttcacgtaca gttccagcaa tcgcgcctcc gaattcgggc ggaacgactc
    28681 ggctacggtc aacgccgtga tcaagtcggg caccaatcag ttccacggaa atgtctggga
    28741 atttaaccgg aacgccggct tgaacgcccg ctcctaccag cagtcgaacg tgaaccccaa
    28801 gctggtgcag aatcaattcg gcggcaccat cggcggaccc gtcctcaaaa acagagcatt
    28861 cttctttgga agttatgagg gatatcggca gacggggtca gccttctcgt ttgcggctct
    28921 cgtccccacc gaggcggagc gccagggaaa cttcgcaggg gtaacgcccg ctccggttga
    28981 cccgagcacg gggcagcctt atccgggcgg ccaggtcccc gtcgatcccg ttatcgccaa
    29041 attcctccaa tacgttccaa aacccaacct cggaaccaat ggctggcagg gcaactctcc
    29101 caacaactca aattacgacg agtatgtcgc acgtggcgat tacgacctga cacaacggca
    29161 gcgactgacg ggaagctttt acaggctgaa taaccacatc gtgagtaatc agggaagtac
    29221 gatctttctg gatccgtcgg ccacggaata cattacctcc ggcaagcagt atcagatcaa
    29281 tgcgtcgcat acttacacat tctcgccaac caggctgaac acggcgcgct atgttttcct
    29341 tgatgtcggc gccagccgtg gatggctgaa ctccaacttc actctgcagt ccctcggatc
    29401 gcagttcagc agcgcatttg cacagccccc ggacatcatt gtgcagaatt actttaactt
    29461 cgacgcgacc aatgatggca ccgtgtacac acacaaccag gaactggaag acacgttcca
    29521 gtggattaaa ggctcccatc agctcaccat gggaggccag ggaatctatt atcacgataa
    29581 ccagttcacc agtccggggc cctggatcta cttttacggt accgccacgg ggaatgctct
    29641 ggccgatttt atcgccggga acgtcggctc atttcaatac agtgtgccga atttgacggc
    29701 agatgtgaca gtgcactggc tgttcagtgg gtttgtcaac gacaactgga aagtaacgcg
    29761 caaattatcc atcaccgcgg gtctgcgatg ggaagatttg acgggcgtca ctaacccgca
    29821 ggattatcgg accacattca agcctaacgt acagtcccag gtcttcccca actatctgcc
    29881 tggctggttg ttcaagaaca cgattacagg aaagaccgac ccggggtgga gtaacgccgg
    29941 atatcaggct ccgcaggaag tggatccccg gattggattt gcatacgatg tcttcggaaa
    30001 cgggaatacc gcggtgcgcg gcggaatcgg aatctacggt ggagaggttg aaacgattgg
    30061 atggcagaac ggcagtccat tcgccaatcc gtcgcccagc tgcttcacgg gcaatcagac
    30121 gtttgttccg atcagtaatc cctacgtaaa cacctgcgat cccatcacgg cggcgaaagg
    30181 gtggaccggg ccgcctgccg actactccgc acccatacct tatgcgggtg gcgggcttga
    30241 tcctcgcaca aagaaaccct acgccttcaa ctatagtctc ggcgtccagc accagatcag
    30301 ccaatccatg tatgtcgagg catcctatgt cggcagcctg gtgcgaaaag tgtggttcaa
    30361 ttatgactac ttcggtgggg cgcagtatgc tccaggagcg accgagtcca cacagagcct
    30421 ggccgatcgg tatccttatc ttaccggcaa ggtttcgggt gtgttcgtga atggcactcc
    30481 ggacaacgct tcttaccatg gactgctgat tcagttcaat cggcgcatgc gaagcgggtt
    30541 gcaatttacc acctcctaca cctggtcgaa agcgctggac gcgaaccact ggccggtgca
    30601 gaactgggcg gtgccacgga tgcgatgggg catcagcgac agcgacttcg ccagtaacct
    30661 tgtaggcagc tttgtctatg agccgcccgt tcatctggac aatcacttcg cccaggcaat
    30721 tctgagcgga tgggagctta cgggactagt ccagttccag gatggtcagc cacttacagt
    30781 cctgaccgga accgacaatc tggtgaacag ctactacggt tcgcgtccta accaggtcag
    30841 cgatccgact ctgagcgcac accgttcacg gccggcggag atggcgaaat ggttcgacac
    30901 cagcgcgttc gccgatccag ggattggggt tgcaggaaat atcggagtcg acaccctgcg
    30961 cggtcccggc aggaagaata tcaacgcgtc cctgctgcgg agcattccaa taaccgagca
    31021 tgcacggttc gaatttcact tcgacacgtt caacaccctc aattgggtga atctcggcaa
    31081 tcccaacagc acaatgaaca gtccgaattt cggaaagatc acgagtgccg gagccatgcg
    31141 tcagttgcag cttggcggaa aaatcaactt ctaactgtgg ggacgaggga gttcaactgc
    31201 tccctcgtcc tcatttcatc cgatagatcg aaagaggatg atgttccccg gatctgatcg
    31261 ttgttcggca ctgagaggaa tcggaggatt gttgtttcgc tccctcagtg ccgttgctct
    31321 ctcaatcgta ttgtggacga cgtcatttgc gcagacgaat gacacatcgc cgtttgaccg
    31381 gctaagccgg cctgacaaga cggaactgac cgacgcattc agtaacgttg ctgctaccgg
    31441 tggtgagttt gggctgccgt ggatgtggtg gtggcatcgg attaatgcgg gatggggtac
    31501 ggtgcgtccg gaagtcgcgc gtttggtcgt ctatgacgag cgcagccatc agaccatgtg
    31561 gctgcagggc ggagaacgca cctggacggc ggatcatctc catcaaagcg caaagctgaa
    31621 cggactcacg ctttctgaag ataagtctgt cgtcggcgac tcggtggccg acgtcctgcg
    31681 cattacaaac ggttccgccg aagcacgctc actcaaactg tactttctcg gccatcctga
    31741 ggcgcattcc ccaggcaatc ctgacagccg cggcctgtcg attgcgtttg acccatcctc
    31801 caattcgatc cgtcttaccg aggaaaagag atacggtcag cactatttac caggagtgat
    31861 agagatcgac cagcggatcg gcagttctct ccccatttcc ggatgggcta tcggaacttt
    31921 cgatggcgac gtggagaagt ttcttggccg tgacctcggg atgggcgcgt tcat
//
LOCUS       DASZYN010000850         1834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_636231, whole genome shotgun sequence.
ACCESSION   DASZYN010000850 DASZYN010000000
VERSION     DASZYN010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636231"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..1695)
                     /locus_tag="VHE33_21255"
     CDS             complement(268..1695)
                     /locus_tag="VHE33_21255"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HVU00041.1"
                     /translation="MRLAALDVEALIEANHPARLIWKLSEEFDLSQFEARQKSREGEA
                     GRPCWSAQLLISIWVYSYSMGVASARAIERMMEYEPGLRWLSADQVINHHTLSDFRVG
                     HQTALENLFAELLGMLDAAEVIDMTTLLHDGTKVRAVAGKYSLHRRKKLEERVKQARQ
                     LVKKLDAAAAENEEMDKKRQAAQSRVAHEALSRAQSALKKLKKKQAQTRASERDQVRV
                     SESEPDAVKMKHPDGSWAPSYNVQVSTEAKSRMIVAIGLTTDPNDQHQLMPALERVKK
                     DCGVLPRQIIADNGYVSRPNIEETTKAGVELIAPWKVDTSREAGACKVNGIASEFAPS
                     QFRMEKSSQCLICPEGKKLVAIGEKVHYSVRRKIYQAKAADCAGCERRAECCGSRSGP
                     RRVHRVMESEAMQQYLDRMQRPEVAQLYKRRCEIAEFPHLWTKGVKKLSRFKVRGLMK
                     AAMEATWIAIAYNVTQWMRLGLAAA"
BASE COUNT          309 a          600 c          457 g          468 t
ORIGIN      
        1 tcccgaatgg gaaattcgct gaaaaacgct ctgaccgctg aaacgactct gttaccgagc
       61 cgccgttacc gagccgcgac cgcaagggcc tgtgaaataa tcaattttcg gaagagctgg
      121 cttggcacgc tggcgcctca cgacgttgct tgaattttgc gggttaattc tcgaagattg
      181 gctggccgcc gcttgtgccg gtttttaggc tctgaaacaa tgattttgtt cgattgggcc
      241 gtgtggcgaa gccctctggt ttctcagcta agccgctgct aagcccagcc gcatccactg
      301 ggtcacgttg tacgctatgg cgatccatgt ggcttccatg gcagctttca tcaaccctcg
      361 cactttgaag cggctcagct ttttgactcc cttcgtccac aggtgcggga actcggcgat
      421 ctcacatcgc ctcttgtaaa gttgagccac ttcgggccgc tgcattcgat ccaggtactg
      481 ctgcatcgcc tcgctttcca tcacgcgatg cactcggcgc gggcccgagc gcgatccgca
      541 acattccgca cgccgttcgc atccggcgca gtccgccgcc ttggcttgat atattttgcg
      601 gcgcacgctg taatgcacct tctcaccgat cgccaccagc ttttttcctt ccggacagat
      661 caggcactgg ctggatttct ccatccggaa ctgcgatggc gcaaactccg atgcgatgcc
      721 gttcaccttg catgctccgg cttcgcgtga ggtatctacc ttccacggcg cgatcaactc
      781 tactccggct ttggtggttt cttctatgtt cgggcgcgat acatatccgt tgtcggcgat
      841 gatctgccgg ggcaaaactc cgcagtcttt cttcacccgc tccagcgctg gcatcaactg
      901 atgctgatcg ttggggtccg tggtgagccc gatcgcaacg atcatgcgcg atttggcttc
      961 cgtgctcacc tgcacgttgt aactcggcgc ccaggaaccg tcggggtgtt tcatcttcac
     1021 tgcgtccggt tcgctttcgc tcacccgcac ctggtcgcgc tcacttgccc gggtctgggc
     1081 ctgctttttc tttagttttt tcaacgccga ctgcgcccgg ctcagcgcct cgtgcgccac
     1141 ccggctttgc gccgcctggc gctttttatc catctcttcg ttttccgctg ccgccgcatc
     1201 caacttcttg accagctggc gcgcctgctt cacccgctct tccagcttct tccgccgatg
     1261 cagcgaatac tttcccgcca cggcgcgcac cttggttccg tcgtggagca acgttgtcat
     1321 gtcgatcact tccgctgcgt ccagcattcc caataactcg gcaaatagat tctccagcgc
     1381 cgtttgatgt cctacccgga aatcgctcag cgtatggtgg ttgatcacct gatccgccga
     1441 cagccaccgc agtccaggct cgtattccat cattcgctcg atggcccgcg ctgacgccac
     1501 acccatgctg tagctataca cccagatgct aatcagcagt tgcgccgacc agcacgggcg
     1561 accggcttcg ccctctcggc tcttctgcct ggcttcgaac tgactcagat caaactcctc
     1621 gctgagtttc caaatcagcc gcgccgggtg gttggcttcg atcaaggctt ctacatctaa
     1681 cgccgccagg cgcacttggg cccgatccag gtcgatcgtt ttgaccgacg cttttgggac
     1741 ttctctggta gtctccacat cctgagctta aaacaaacgg aaacattttc tgctccaaat
     1801 gtcttaaact cgcgagattt tttcacagac ccgc
//
LOCUS       DASZYN010000851        13817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_887710, whole genome shotgun sequence.
ACCESSION   DASZYN010000851 DASZYN010000000
VERSION     DASZYN010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 13817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13817
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887710"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VHE33_21260"
     CDS             complement(<1..193)
                     /locus_tag="VHE33_21260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00042.1"
                     /translation="MPAQKEQWFAGPGKPVPSVRGFSVLFENDQLIVDPPQPYPGAGD
                     PATDLPELCETLPPQDRARC"
     gene            341..1786
                     /locus_tag="VHE33_21265"
     CDS             341..1786
                     /locus_tag="VHE33_21265"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HVU00043.1"
                     /translation="MHEADGKTLTRRTLLGGSVAGATLLAGRANAQRKSRQPNVIMFL
                     ADDLGAADIGCYGAPQIRTPAIDAIAAAGARFTQAYANSAVCTASRVGIITGRYQYRL
                     AIGLEEPLSAVSDVGLPPEQPTLPSLLRKAGYSTYLVGKWHLGSLPKYGPLKSGYDHF
                     WGFRGGALDYFSHQSNGKPDLWDDDSAVESKGYLTTLIGGRAAEIVRSQAASNKPYFI
                     SVHFNAPHWPWEGPEDEAHSKEIGPNISDPQGAKLATYNRMVEAMDQQIGAVLRALEQ
                     TGQADNTIVIFTSDNGGERLSNTWPFTGKKTELLEGGIRIPSIVRWPGRFRADLVSPQ
                     VMIHMDWLPTLLAACGGQPDPGSPSDGINLLLKLTEGVAATPRTLFWRYKSNQQRAVR
                     DGDYKALKIGTHGYLFNVVDDPLERSDLKTKHPDIYQRLTGAWNDWNRAMLSETADAN
                     TYNNSAATWADHINTPPIDRRAYDDGSPWPL"
     gene            complement(2038..2799)
                     /locus_tag="VHE33_21270"
     CDS             complement(2038..2799)
                     /locus_tag="VHE33_21270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVU00044.1"
                     /translation="MSDLNGRLALVIGAAAGIGLASARALADKGAAVILADRNGDAAE
                     LAASGLRASGAEAVSYETDASSLPQLRGLFDFVDKTYGKLNVLFANVGMRGPDGFDVT
                     EEQFDEVFNVNLKSHFFAANCAIPLLRRCAPQASMIFMSSAGGLRVGDRCPLYAISKA
                     GVVMMARAFARKLGPDGIRVNALCPGAIETAFPRWSGLGEAEYQAAVDRVGRNVPLRR
                     IGQPQDVAGVVTFLASDQSQYLTGLSIPIDGGELA"
     gene            complement(2856..3422)
                     /locus_tag="VHE33_21275"
     CDS             complement(2856..3422)
                     /locus_tag="VHE33_21275"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HVU00045.1"
                     /translation="MPITALDPKAALIVVDLQKGIVGSAPTVHPAAEIVQRASLLAAA
                     FRRHGLPVVLVNVAGRAPGRNEQSLPAGGARPTDWADLVPELNRQPEDHTVTKQTWGA
                     FTNTDLAEYLKTRGVTQVVLAGIATSIGVESTARQAHELGLNVTLSIDAMTDTNAEMH
                     AISTRHVFPRLGETGTSAEIIALLDKRA"
     gene            complement(3479..4870)
                     /locus_tag="VHE33_21280"
     CDS             complement(3479..4870)
                     /locus_tag="VHE33_21280"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HVU00046.1"
                     /translation="MPKDVEVIMPLPTQRRIGPILLAPGVSPKQVLVFLFVTSICVFV
                     NEFASVMQPLIFALQLHIAPGQQGVLAGMLGATQQTGMLIFIIAAGALADIFGRKVML
                     LYVLIGFFLCLVAYPLIPAVWALFIVRFVWGLSFTGFSAGAATISMDIPDNCARGKLN
                     GLVLVSNLLVASLLVLPAARLPALFRGLGLDAHAALAYTFGFVALIASVGLLTVIFFF
                     HEQRKKPQSAAPELLLSKSKAIFTNVRAVLAHACQDKRFAVILMIGSVVRTDAVIVSA
                     FLGLWIVNAGSLGGIDAIEATKMAGLVASIRFVAKVTGALLFGVIADKVNRMWLMLVA
                     LTMTTLAFGGFGLVSNAFGMGMIAMAVLIGLAESAEGVTVQSLIAQEAPEHLRGSSMG
                     VFTFLGTISLLVVNLAGGYLFDRIGFSSPMLMEGALHLMVLITAIVILRMGTDGVAGA
                     PPSANILVAKTGR"
     gene            complement(5336..7237)
                     /locus_tag="VHE33_21285"
     CDS             complement(5336..7237)
                     /locus_tag="VHE33_21285"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HVU00047.1"
                     /translation="MKNRFYPHAFLLAALLLPAAPRAEGQDWSDFNGNVMGQKYSTAS
                     QITPANVKNLGVAWSLHTGDVSYGGAGKMPKTQWQSTPLFVNNTVYVSTPFFRIFAIE
                     PDTGKVKWIYAAHTMSNEPNAKFRGRNRGIAYWAVANPVAGQHCQKMLYIGTVEGQLH
                     AIDADSGQLCQGFGNHGILNVNQWNTVNPKFPFELNQPPVVYKDMLVLGWAGIDSLWQ
                     ASPPGSVFGLDARTGTKRWEFQTIPENMRQKIGTANVWAAMSVDVANGMVYLPVSSPE
                     NNFYGGDRLQDMPYATSVTALHADTGQVAWARQLVHHDLWDLDTNSAPTLVDIPKGGK
                     TIPALVQATKQGFLFVLNRLTGEPIYPIVEKPVPKSNVPGEIASATQPWVPTPAPTVG
                     DRFPGIFKLADWAGGGQCSRDYASYRDEGRFTPPSMGGTVVMPPTAGGVEWGGGAVDP
                     ATQTYVVNSSNLVRIYQLMARNEFNALEKKIGPAAGSPNFGSPYASRTFNFFNKWGMP
                     CWNPPYGTLSSYDLKTGKLLWRHPFGQVQQWGFYMPESWGSVTIGGPVITKSGLIFIG
                     ASMDSRVRAVDLKTGNVLWKHMVAAPAVATPAIYTYRGRQYILFAAGGNNLVSPRVGD
                     QLVAFTLPN"
     gene            complement(7234..7587)
                     /locus_tag="VHE33_21290"
     CDS             complement(7234..7587)
                     /locus_tag="VHE33_21290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00048.1"
                     /translation="MGSLSCGRGTWCIVLASITGLAIALYDYVTPETGIDQSGGVLLV
                     IVAMGLMLCAALSVALLGSGLVAGILFFLILLDIVGTAIAGYFLESPMLMGAMVIAAI
                     GWFRCLVVSAQRNWQ"
     gene            complement(7748..8848)
                     /locus_tag="VHE33_21295"
     CDS             complement(7748..8848)
                     /locus_tag="VHE33_21295"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HVU00049.1"
                     /translation="MASRNHEYFFGQTPRAIIQHRVEVVISRTYCPDRREALASGLAL
                     TVLAVAPATPVTAASLSEIKLFDGHLHLVSEDLQRYPRASGPPPDGAAPPGSSPAPGS
                     SIEVGKPRLMSPVEQVLGWMDEASVDTAAAIQRVSSYGLDNSYVLDSAAAHPDRFCSV
                     VVLNARDAATPSRIGELAKAHKIAGLRLTGGKDASGRYPWMESDEALKTWEALDANGL
                     VMDLGFSPQIFTLEALEATLRMARMFPRVNIVLDHMGRPKTEGAPGYGFSDYPAGLSS
                     QRNVFFKFTTQNLYVLEEANIPATDFVHNIVHRFGAGRILWGSNMGTSAGTYAEMAQR
                     ARLATASLTVAQQRLVLRETGRAVFMRAAHPT"
     gene            9005..9145
                     /locus_tag="VHE33_21300"
     CDS             9005..9145
                     /locus_tag="VHE33_21300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00050.1"
                     /translation="MQEAQAAANWAFHPPLGHRSNGGGGANMWSKLSGGNYRQTINDN
                     LI"
     gene            complement(9142..11457)
                     /locus_tag="VHE33_21305"
     CDS             complement(9142..11457)
                     /locus_tag="VHE33_21305"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HVU00051.1"
                     /translation="MSVVDTNTIESAASSGIALADILRKAADLAPEAGMARRSFFKLS
                     LGTGAGLVLGFYLPGGDAARAANASMPAVLNAFVRIAPDNIVTLYSISPEIGQGIKTT
                     FGLMIAEDLDAEWKSVRVEQARISPLIYGDQQRAGGSTSVRRNWNSLRQAGASARAML
                     VAAAAREWTVPVSEITTQNSTVMHKASGKTITYGALAEKAALMPVPDPATVKLKDRKN
                     WRLLGKRYTGVDNKKIVTGQRLFGIDVRLPGMLYANYTKCPAPNGKVKSANLEEIKAL
                     PGVKDAFILEGSGFPAQAGDVMPGVAIIATSTWAAFSAKKALKIEWDESSAAKDSTTQ
                     AGVQARQLKAGPLPKPYTNIGDVDAAFASAAKTVEADYEYAVVAHANLEPMNATAWWH
                     DGVMEMWLPTQEPNRGMPQIARIAGVPQDSVLINQTRAGGGFGRRQVNDFSCEAAAIA
                     LRVNAPVKLQWTREDDFAHDFFRPGGFHRVKGAIGKDGKIDAWQNHIISFSQDGKEEV
                     HAGGMSGNFFPVGHVPNVRFARSLISPYLTPTGSMRAPTYNVLIFVQQGFLHELSVAA
                     GRDHLEMLLETLNRSDVAPAPNDAPRPGSPPPVIYSASRATDVVRLVAEKSGYGRKRP
                     KGSGLGLAFSFGLGGHVATAVDLSVDDKKHITVHKITVALDVGPIVNMSGAENQAQGA
                     SLDGLSTTMGWAIDIENGRVQQANFNSYPLMRARSAPPVDAFFVQSDNMPSGMGEPAY
                     PGVSPALANAIFNATGERLRQMPFSKLGYTI"
     gene            complement(11444..11965)
                     /locus_tag="VHE33_21310"
     CDS             complement(11444..11965)
                     /locus_tag="VHE33_21310"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HVU00052.1"
                     /translation="MLHLTVNGQDRTVADDVDPATPLLWVLRDTLALEGTKYGCGGGY
                     CGACTVQLDGVAVRSCQIPVQSVGAKSITTIEGLAGADGTLHPLQEAWIDYDVPQCGY
                     CQAGQLMTAASLLKQYPAPTDAQIDQAMNGNFCRCGCYNRIKAAIHTAAGKAPTVEPA
                     LRSAMEGEPHVGG"
     gene            12420..12590
                     /locus_tag="VHE33_21315"
                     /pseudo
     CDS             12420..12590
                     /locus_tag="VHE33_21315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612082.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            12680..13791
                     /locus_tag="VHE33_21320"
     CDS             join(12680..12926,12926..13791)
                     /locus_tag="VHE33_21320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611490.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HVU00053.1"
                     /translation="MRSSRYTEEQIIGILKQHQAGLGAKELCRKYGISDATFYKWRSK
                     YGGMEVSDARKLKALEEENRKLKKLLAETALDAATLKENAGKKLLTPRTRRVAVSWAI
                     QEKGYTQRRACRLVGLEPKTYRYASTRPDDGPLRQRLKELASERRRFGYRRLHLLLRR
                     EGVLLNWKKLYRLYREERLMVRKRGGRRRALGTRAPMTIPQGRNQRWSLDFVSDTLAS
                     GRKFRMLTVVDDFSRECVAVVVDNSLSGIRVARELDQVLEMRGTPCMVVSDNGTEFTS
                     RAILAWQEERGVEWHYIAPGKPTQNGFIESFNGRLRDECLNEHLFASLPEARRIIEEW
                     RIDYNTLRPHTSLDGLTPTEFAARPSQGHNQNRLSP"
BASE COUNT         2975 a         4134 c         4083 g         2625 t
ORIGIN      
        1 cgcaccgcgc ccggtcttgt ggaggcaagg tttcacaaag ctcgggaaga tctgtggcgg
       61 gatcgcccgc gcccgggtat ggctgcggtg gatcgacaat cagctggtca ttctcaaaca
      121 ggacggaaaa accccgtacg ctcggcaccg gcttgcccgg cccggcgaac cattgctctt
      181 tctgggccgg caccgacgcg gtttgggcgg ccagcggacc gcccgcaaga caaagcggac
      241 atcgcggacg agtttgggct cagagaataa cgtttagggc ttattgcttg cactcgccgg
      301 atatgactaa aattgcagga accaggctgt gggggcagtt atgcatgagg ccgatggaaa
      361 aactctgacc cgccgtactt tacttggcgg cagcgtcgcg ggggcaacgc tgctggcagg
      421 gcgcgcaaat gcccagcgca agagccgtca gccaaatgtg attatgtttt tggctgacga
      481 tctgggggca gcggacatcg gctgttatgg cgcgccgcag attcgaaccc cagcgattga
      541 tgcaatcgcg gcggcaggcg ctcgatttac gcaggcctac gccaattcgg ctgtgtgcac
      601 ggcttcgcgc gtcggcatta tcactggccg ctatcaatac cggctggcca tcggcctgga
      661 ggagcccctc tccgccgtct cggatgtcgg gttgccgccg gagcagccga cactaccgtc
      721 cctgttgcgg aaagccggat attccaccta tctggtcggc aagtggcacc tcggttctct
      781 gccgaaatat ggccctctga agagtgggta tgaccatttc tggggctttc gcggcggtgc
      841 cctggattac ttctcgcacc aatccaacgg taagcctgac ctttgggacg acgacagcgc
      901 agtcgagtct aaggggtatc ttacgacact gatcggaggg cgggctgccg aaatcgttcg
      961 ctcccaagcc gcaagcaaca agccctattt tatcagcgta catttcaacg cgcctcactg
     1021 gccgtgggaa gggccggaag acgaagccca ttcgaaagaa atcggaccaa acatcagtga
     1081 tcctcagggg gccaagctcg caacctacaa cagaatggtc gaagccatgg accagcagat
     1141 aggtgccgtg ctccgcgcct tggagcagac cgggcaagcg gataatacaa tcgttatttt
     1201 caccagcgac aatggcggcg agcgcctttc caacacttgg cccttcaccg gaaagaaaac
     1261 tgagttgcta gagggcggca tccggattcc atccatagtc cgttggccgg gccggtttcg
     1321 tgcggacctg gtaagcccgc aggtgatgat tcatatggac tggttgccga ccctgctggc
     1381 ggcgtgcggc ggccagcccg atccggggtc tcccagcgac ggaattaacc tgctgctaaa
     1441 gctgaccgaa ggcgtcgcgg cgacaccacg gacgcttttc tggcgatata agtcgaacca
     1501 acagcgcgct gtgcgcgacg gcgactacaa ggcgctcaag atcggtacgc acggatactt
     1561 attcaacgtg gtggacgatc cgctggagcg ttcagacctg aagacgaagc atcccgacat
     1621 ctaccagcgc ctaaccggtg cttggaatga ctggaaccgc gcaatgctgt cggagacggc
     1681 ggatgcaaac acctacaata acagtgctgc gacctgggcc gaccatatca acacgccccc
     1741 gatagaccgg cgtgcctatg atgatggcag cccctggcca ttgtaattga cttgggccac
     1801 ccagccccgc agttgccgta gcttcgcgac aatctcttcc gctgcgtgcc ttttccttgc
     1861 catcaaccca atcctccttg gtcaaaacca tacttcaggg aggaccattt caaagggggc
     1921 aggacactcg cccccgcaca ctgccttcgc gaataggcgg aaccgcctcg tcgaagcgat
     1981 atgtctgtat ttcagcgctc attttattcc cttccctaaa ggacaccgag aaatatttca
     2041 ggccaattcc cccccatcaa ttggtatcga taatccggtc aggtactggg actggtccga
     2101 tgccaggaaa gtcaccacgc cggcgacatc ctgcggctgg ccaattcgtc tcagcggaac
     2161 attccggcct acgcgatcaa cggcagcctg gtattccgct tcgccaaggc cagaccagcg
     2221 tggaaatgcc gtttcaatcg caccgggaca cagcgcgttg acgcgaatgc cgtcgggccc
     2281 caatttccgc gcaaaggccc gcgccatcat cacgaccccc gccttgctga tcgcatagag
     2341 ggggcagcgg tcgccgaccc gcaatccacc ggccgaagac atgaagatca tcgatgcttg
     2401 cggcgcgcag cgtcgcagga gcggaatagc gcaattggcg gcgaaaaaat gacttttcag
     2461 attgacgttg aacacttcat cgaattgttc ctccgtaacg tcgaagccat ccggtcctct
     2521 catgccgaca tttgcgaata ggacattgag ttttccatac gtcttgtcga cgaagtcgaa
     2581 caggccgcgc aattgaggca acgaagacgc gtcggtctca tatgagactg cctcggcccc
     2641 ggaagcgcgc agaccgctgg cggccagctc cgcggcgtca ccattcctgt ccgcaaggat
     2701 gacggcggcg cctttatctg ccagcgcgcg cgccgacgcc aggccgattc cggcggctgc
     2761 cccgatcacc agtgccagcc tgccattcag atcagacatc ccacgatctc ccataggctt
     2821 agaggccgcc ccatgcgccg gaacgcaagc gctttctagg cacgcttgtc cagcaacgca
     2881 atgatctcag cggatgtgcc ggtctcgccc agtcggggga aaacatgcct ggtgctgatg
     2941 gcatgcatct cggcattcgt gtccgtcatg gcatcaatgc tgagggtgac attcaatccc
     3001 aattcatgcg cctgacgcgc cgtcgattcg acgccaatgc tggtagcgat gccggcgaga
     3061 acgacctgtg tcacgccacg tgtcttgaga tattccgcga gatccgtgtt tgtgaaggcg
     3121 ccccatgtct gcttggttac ggtatggtct tcaggctggc gattgagttc tggaacaagg
     3181 tcagcccaat cagtcggccg cgcgccaccg gcaggcagag actgctcgtt tcggccgggc
     3241 gctctgccgg cgacattcac caggacgacc gggagaccat gacgacggaa tgccgcggcc
     3301 agcaggctag cacgctgaac gatttcggcc gcgggatgca cggtcggagc ggagccgacg
     3361 atgcctttct gcagatccac gacaatgagc gcggccttgg ggtctagggc tgtaataggc
     3421 atgtcgtctc catcttgtga ttgaatgaac caaaacgcac gtctggacgc atgtggacct
     3481 agcgaccagt ttttgcaacc aggatattgg ccgaaggcgg ggcgccggcc acgccatccg
     3541 tacccatgcg cagaatgacg attgctgtaa taagcaccat cagatgcaag gcgccttcca
     3601 tcagcatggg ggacgaaaat cctatcctgt cgaatagata gccgcccgcc aagtttacca
     3661 ccaaaaggct gattgttccc aggaaggtga acacgcccat ggatgatccg cgcaaatgct
     3721 cgggtgcctc ctgggcaatc agggattgca ccgtcacgcc ctcggcgctt tccgcaagac
     3781 cgatcaggac agccatcgcg atcattccca tcccaaaagc gttcgaaacg agcccgaagc
     3841 ccccgaaggc gagcgttgtc atggtgagcg ccaccagcat cagccacatc cggttcacct
     3901 tgtccgcgat gacgccgaac agcagggcgc cggtaacctt ggcgacaaat cggatggagg
     3961 cgaccagccc cgccattttt gtcgcctcga tggcatcaat gccgcccaga ctgcccgcat
     4021 tgacgatcca tagacccaag aaagcgctga caataacggc atcggtgcga acaacgctgc
     4081 cgatcatgag gataacggca aacctcttgt cttggcacgc atgggccaat actgcccgga
     4141 cattcgtaaa tatggcctta gacttcgaca gcaggagctc aggagccgcc gattgtggtt
     4201 ttttccgttg ttcgtgaaaa aagaagataa ccgttaggag cccaaccgat gcgatcaggg
     4261 caacaaatcc aaaggtgtag gcaagtgccg catgggcatc gaggccaagg ccgcggaaaa
     4321 gagccggcag ccgcgcagcc ggcagaacca aaagcgatgc cacgagaaga ttggacacca
     4381 gcacgaggcc gttcagtttt cccctggcgc aattgtctgg aatatccatc gaaatggtgg
     4441 cggcgcccgc cgagaaaccc gtaaacgaca ggccccacac aaaccggacg ataaaaagcg
     4501 cccaaacggc cgggatcagg ggataggcga ccagacatag gaaaaagcca atcaagacat
     4561 acaaaagcat gaccttgcgg ccgaaaatat cggccagcgc cccggcggca atgatgaata
     4621 taagcatgcc ggtttgctgg gtggcgccca gcattcccgc cagaacgcct tgctgccccg
     4681 gtgcgatgtg cagctgcagc gcaaatatca ggggctgcat cacactcgcg aactcgttca
     4741 cgaatacgca gatgctggta acgaatagga atacaagaac ttgttttgga ctcacgccag
     4801 gcgccagaag gatcggccct atccgccttt gtgtggggag aggcatgatg acctccacat
     4861 ctttcggcat cgaaattcaa cctcggatgg atcggaaatg ttctcgggca atcgttgcca
     4921 acgcctgttc aatcacttcg ccctcgaggc aaccagtcga aacagatcca gcaaaggctc
     4981 cgtcatcgcg caccacaatg tggctgccca gttgccttgg agtcgatcca gagattttca
     5041 ctacggtagc tagggcaaca gcgtgaccgc gcgagcgcca tccatttgcg atagaaagca
     5101 catcaaattc ggaggtgggc aataacattg ctgcaatttt cttccaagga acctgagcgc
     5161 ggttagccta tgatcctagc cggttttttt cgactggcca ataagacaag cgctggttgg
     5221 agaacagcgg aaacctggcc aggaccctgc aagatgcccc gtacgaccgg ctggaatttc
     5281 cgagaagaag tcaaacttcg ccggcatcgg cgtcagcaat gttccgtgac tggccttagt
     5341 tcggcagcgt gaacgccact agttgatcgc ccacgcgggg cgagacaaga ttgttgccgc
     5401 ctgcagcgaa caggatgtac tgccggcccc tataggtgta gatggcgggc gtcgccacgg
     5461 cgggagccgc gaccatgtgc ttccagagca cgtttccagt cttaaggtcc accgcgcgca
     5521 cacggctgtc catcgaggcg ccgatgaaga ttaggccaga ttttgtaatc accggaccgc
     5581 cgatcgtcac cgagccccag ctttcgggca tgtagaagcc ccattgctgc acctggccga
     5641 agggatggcg ccacagcagt ttgccggtct tcagatcata ggaagacaga gtgccatagg
     5701 gcggattcca gcacggcatg ccccatttat tgaagaaatt gaaagtgcgc gaggcatagg
     5761 gagagccgaa attcggcgaa cccgctgcgg gtccgatttt cttttcgagg gcgttgaatt
     5821 cgttgcgcgc catcagttgg tagatccgga ccagattaga actgttcacc acataagtct
     5881 gggtcgctgg atccactgcg ccgccgcccc attcaacgcc gccggcggtg ggtggcatca
     5941 cgacagtgcc gcccatcgaa ggcggcgtga agcggccttc gtcgcgatag ctcgcataat
     6001 ctcggctgca ctgcccaccg ccagcccagt cagccagctt gaatattcca gggaagcgat
     6061 cgcccacggt cggcgcggga gtcgggaccc aaggctgggt cgcagacgcg atttctcctg
     6121 gaacgttgga cttcgggact ggcttttcta caatgggata gatcggctcg cctgtcaggc
     6181 ggttcagcac gaacaggaag ccctgcttgg ttgcttgcac gagggcagga attgtcttgc
     6241 cccccttggg aatatccacc agcgtggggg ccgaattggt atcgaggtcc cagagatcgt
     6301 gatgcaccag ttgccgcgcc caggcgacct ggccggtgtc ggcatgcaat gccgttaccg
     6361 atgtcgcata aggcatgtcc tgaagccgat caccaccgta gaaattattc tcgggcgagg
     6421 acacgggcag atagaccatt ccgttcgcaa catcgaccga catggccgcc cagacatttg
     6481 ccgtgccaat tttttggcgc atattttccg gaatagtctg gaattcccat ctctttgtgc
     6541 cggtgcgggc atcaagaccg aacaccgatc ccggcggtga ggcctgccat agcgagtcga
     6601 tgcccgccca ccccagaacc agcatgtctt tgtagacaac gggcggttga ttcagctcga
     6661 agggaaactt tggattgacc gtattccact gattgacatt cagaatgccg tgattaccaa
     6721 aaccctggca gagctgaccg ctgtcggcgt caatggcatg caactgacct tcgacggtac
     6781 ctatgtagag catcttctgg cagtgctgac cggcgacagg attggctact gcccaataag
     6841 cgatgccgcg atttcggcca cggaatttgg cattcggctc gttggacatg gtatgggcgg
     6901 catagatcca cttgaccttg ccggtatcgg gctcgatcgc aaagatgcgg aagaaaggcg
     6961 tagagacgta aaccgtgttg ttgacgaaca ggggggttga ttgccactgc gtcttgggca
     7021 tcttgccggc gccaccatag gaaacatcgc cagtatgcaa ggaccaggcg acaccaagat
     7081 tcttcacatt ggccggagtg atctggctgg cggtggaata tttctgtccc atcacattgc
     7141 cattgaaatc ggaccagtcc tggccctcgg ccctcggggc cgccggcaac agcaaggcgg
     7201 cgagcaggaa ggcgtgcgga tagaatcggt ttttcattgc cagttccttt gcgcgcttac
     7261 aacaaggcac cgaaaccagc cgatggcagc gatcaccatc gcgcccatca gcatggggct
     7321 ttccagaaaa tatcccgcaa tggcagtgcc cacgatgtcg agcaggatga gaaaaaagag
     7381 gatgcctgcc accaagcccg aacccagcaa ggccaccgac agggccgcgc atagcattag
     7441 gcccatcgcg acgattacta gcaacacgcc accggattgg tcgataccag tctcaggcgt
     7501 gacgtaatcg tagagcgcga tcgccagacc tgtgattgac gccaaaacga tacaccaggt
     7561 accgcgcccg cacgacaaac tgcccatgcc atacctcttc acaagcgtta ttgaaatgtg
     7621 agacgcagac ccaaatgatg aagcgttcac aaaaacatgc tccgtcccag gcatctgccc
     7681 ccccgacaac cgcagcagtc catgactcac acaggcgaca aaccgggtcc tctggagatt
     7741 ccatgatcta cgtgggatgc gccgctcgca tgaagaccgc gcggccggtt tcacgaagga
     7801 ccagccgctg ttgagcgacg gtcagggaag ccgtggctag gcgcgcgcgc tgcgccatct
     7861 ccgcataagt gccggcggac gtgcccatat tcgagcccca gaggatacgg ccggccccga
     7921 accggtgcac gatattgtga acgaaatcgg tggctgggat attcgcttcc tccaaaacgt
     7981 acaggttttg ggtcgtaaat ttgaaaaaga cgttgcgctg gctggatagc cccgccggat
     8041 agtcagaaaa accatatccc ggcgcccctt cggtcttggg ccgccccata tggtccagca
     8101 cgatattcac gcgcgggaac attcgtgcca tccgcagggt ggcctccagc gcttcgagcg
     8161 taaagatttg cggcgagaaa cccagatcca tgacaaggcc attcgcgtcc agggcctccc
     8221 aggtcttgag tgcctcgtcc gattccatcc acggatacct gccgcttgcg tccttgccgc
     8281 cagtcagcct caggcccgct attttgtggg ccttggccag ttcgccgatg cgacttggcg
     8341 tcgctgcatc acgtgcattg agcaccacca cagagcaaaa gcgatccgga tgggcagccg
     8401 cggaatccag aacataactg ttgtcgagac cataactgct gactcgctga atggcagcgg
     8461 ccgtatcgac acttgcttcg tccatccagc cgagcacctg ctcgacaggg gacatcaagc
     8521 ggggtttgcc gacctcaatg cttgaacctg gtgccgggct tgacccaggc ggtgccgcgc
     8581 cgtcgggcgg tggtcccgat gccctgggat agcgttgcaa gtcctctgaa accagatgca
     8641 gatgcccatc gaataatttg atttcgctga ggctggcggc cgtgacaggc gtcgccggcg
     8701 ctacggccag cacggttagg gcaagacctg aagccagggc ctcgcgccga tcaggacaat
     8761 atgttcttga aataacgacc tccactcgat gctgaataat cgctcttggc gtttgaccaa
     8821 aaaaatattc atggtttcgc gacgccacga atctggaaga gcggtacaga ttgaccggcc
     8881 cccaactgag tagtccagtt gcgatgttag tttagtgacg gggtttgcgc tagcgccctg
     8941 ctggcggact gattcgacaa ccagcagatt gcctctgctg ctggcggcgg ctgatatccc
     9001 agcgatgcaa gaggctcaag cggctgccaa ctgggctttc catccgccgc tcggacatcg
     9061 cagcaatggg ggcggcggcg ccaacatgtg gagcaagctt tccggcggta attatcgcca
     9121 gaccatcaat gacaatctta tctagatcgt atagcccaac ttgctgaacg gcatctggcg
     9181 caggcgctca cccgtggcgt tgaagatcgc attggccagc gccggggaaa ccccgggata
     9241 ggcgggttca cccatgcccg acggcatgtt gtcggactgg acgaaaaagg catcgaccgg
     9301 cggcgcgctg cgggcacgca tcaggggata ggaattgaag ttcgcctgct gcacgcggcc
     9361 attctcgata tcgatcgccc agcccatagt ggtggaaagc ccgtccaggg aggcgccctg
     9421 cgcctggttt tcggcgccgc tcatattcac aatcgggccg acgtccagcg ccaccgtaat
     9481 cttgtgaacc gtaatgtgtt tcttgtcatc gacgctcaga tcaaccgcgg tggccacatg
     9541 gccgcccagt ccgaagctga aggcaagacc aagaccgctg cccttgggcc gctttctgcc
     9601 atagcccgac ttttcggcca ccagcctgac gacatcggtc gcgcgactgg cgctgtagat
     9661 gacgggcggc ggagacccgg gacgcggcgc atcgttcggg gccggcgcga catcgctgcg
     9721 gttcagggtt tccagcagca tctccagatg atcgcggccc gcggccaccg aaagctcatg
     9781 caggaaaccc tgctggacga agatgagcac attataggtc ggcgcccgca tcgaaccggt
     9841 cggagtcaaa tagggagaaa tcaggctgcg cgcgaagcgg acattgggga catgcccgac
     9901 cgggaaaaaa ttcccggaca tgcccccggc atgcacttct tcctttccgt cctgcgagaa
     9961 ggaaatgatg tggttctgcc aggcatcaat cttgccgtcc ttgccgatgg cgcccttgac
    10021 cctgtgaaag ccgccgggac ggaagaaatc atgcgcgaaa tcgtcctcgc gcgtccattg
    10081 cagcttgacc ggcgcgttca cgcgcagggc gatggccgcc gcttcacagg aaaaatcatt
    10141 gacctgcctg cggccgaaac cgccgccagc gcgggtctgg tttatcagca cgctgtcctg
    10201 gggaaccccg gcaatgcgcg cgatctgggg catgccacgg tttggttcct gggtcggcag
    10261 ccacatttcc atcacgccgt catgccacca ggcggtggcg ttcatcggtt cgagattggc
    10321 gtgcgcgaca acggcgtatt cgtaatccgc ctcaactgtc tttgccgcgg aggcgaaagc
    10381 cgcatcgaca tcgccaatat ttgtataggg cttgggcagc ggacccgcct tgagctgccg
    10441 ggcctggaca cccgcctgtg tggtgctgtc cttggcggcg ctgctttcat cccattcgat
    10501 cttgagcgcc ttcttggcgc tgaaggccgc ccaggtcgag gtcgcgatga tggcgacgcc
    10561 gggcatgaca tcgccggcct gggcgggaaa gcccgagcct tccaggataa aggcgtcctt
    10621 gacgccgggc agcgccttta tctcttcgag attggcgctt ttgaccttgc cgttcggggc
    10681 gggacatttg gtgtaattgg catagagcat gcccggcagc cgaacatcga tgccgaacag
    10741 gcgttggccg gtgacgattt ttttattgtc gaccccggta tagcgcttgc cgagcaggcg
    10801 ccagttcttg cggtccttga gcttgactgt ggcgggatcg ggaaccggca tcagcgctgc
    10861 cttttcggca agcgcgccat aggtgatggt cttgccgctc gccttgtgca tcaccgtgga
    10921 attctgcgtg gtaatctcgc tgaccggcac tgtccactca cgggccgccg ccgccaccag
    10981 catggcgcgc gccgacgcgc ccgcctggcg cagcgaattc cagttgcgcc gaaccgatgt
    11041 cgaccccccc gcccgctgct gatcgccata gatcaggggg ctgatgcggg cctgctcgac
    11101 ccggacggac ttccactccg catccagatc ttcggcaatc atgaggccga aagtggtctt
    11161 gatgccctgg ccgatttccg ggctgatgga atagagcgtc acaatattat cgggcgcgat
    11221 ccgcacaaag gcgttgagca cggcgggcat tgacgcattc gccgcccggg ccgcgtcccc
    11281 gcccggcaga taaaagccca gcaccagtcc ggcgccggtt cccaggctca gcttgaagaa
    11341 gctgcggcgc gccatgcctg cctcgggcgc gaggtccgcc gccttgcgca gtatgtcggc
    11401 aagcgcgatt ccggaagaag cagcgctttc gattgtgttc gtatcaacca ccgacatgag
    11461 gctctccctc catcgcgctg cgaagcgccg gttcgaccgt cggcgctttg cccgccgcag
    11521 tatgaatcgc ggccttgatg cggttgtagc aaccgcagcg gcagaaattg ccattcatcg
    11581 cctggtcgat ctgggcgtcg gtcggcgcgg gatactgctt gagcagcgac gcggcggtca
    11641 tcaattgtcc cgcctggcag tagccgcatt gcggcacatc gtaatcgatc caggcttcct
    11701 gcaacggatg aagcgtgccg tctgcccctg caagaccctc gatggtggtg atcgactttg
    11761 cgccgaccga ttgcaccgga atctggcagg accgcacggc cacaccgtca agctgcaccg
    11821 tgcaggcgcc gcaatagccg ccgccgcagc catatttcgt gccttccagg gcgagcgtgt
    11881 cgcgcagcac ccagagaagc ggggtggcag ggtcgacatc gtctgcgacg gtgcgatctt
    11941 gcccgttgac agtgaggtga agcaagcgtg cctcctgatt tctgggacgc gaagtctgac
    12001 ctgtttcatc ctggtcttca agccggtcgg aaggcagaaa gggccgctac cgttatgccc
    12061 aaacgcacat aacgatcagg cgcgccagca taaaacgaaa caccatcact caaagacgct
    12121 cgcttcatca aaatatcgcc gcttaacatc aaccaccaga tgaggccaaa cctcccttaa
    12181 tctacgccct cagctgtctc aattttggca acggcccatt tggccaataa cgaggtaagc
    12241 gtatttggac agcttgttca caatctgaac cctccgcgct taccacttcg cttaccactt
    12301 tgagggtcag attgtgctgg acgtcaaaag cgctgtcgag accaactagc gcttaatgcc
    12361 tggttttatt gggctatttt gactcaccgc cagacgtcgc tggacgacgt tagacgttaa
    12421 atggcggaac agggttcacc agccaggcgt tcacgggcgc gttgatcggc gccgacatcg
    12481 ccatcagcat ggatggcaag ggctcctggc gcgacactgt cttcgtcgaa cgactgtggc
    12541 gcaccatcaa atacgaagag gtgtatctgt gggcctacga cagggtcagc atgaacttgg
    12601 ccccgcgcac tgggcatgac ctgctccccg aaattgccct cgatttgagg tagagtccgt
    12661 tccttgaagg agacggacga tgagaagcag ccgatacacc gaagaacaga tcatcgggat
    12721 attgaagcag caccaggcgg ggcttggagc gaaggagctt tgccgcaaat acgggatcag
    12781 cgatgccaca ttttacaagt ggcgttcgaa gtatggcggc atggaggtct cggacgcgcg
    12841 caagctgaag gcgctggaag aggagaaccg caagctgaag aagctgctgg cggagacggc
    12901 gctggatgcg gcgacgctga aggagatgct gggaaaaaac ttctaacgcc caggacgcgg
    12961 agagtagccg tgagctgggc gatccaagag aagggttata cgcaacggcg ggcctgccgg
    13021 ttggtgggcc tggagccgaa gacctaccgc tatgcctcga cgcgcccgga tgacgggcct
    13081 ttgcggcagc gactgaagga actggcttcg gaacgcaggc ggttcggcta tcgacgcctg
    13141 cacctgttgt tgcgccggga gggtgtcctg ctgaactgga agaagctgta taggctctat
    13201 cgcgaggaaa ggctgatggt gcgcaagcgt ggtggccgca ggcgggcgct gggaacacgg
    13261 gcaccgatga cgatcccgca ggggcgcaat cagcggtggt cgctggactt cgtctccgac
    13321 acccttgcct cgggccgcaa gttccggatg ctgacggtgg tcgatgactt cagccgggaa
    13381 tgcgtggcgg tggtcgtgga caactccctc tccggcatcc gtgtcgcccg cgaactggat
    13441 caggttctgg agatgcgggg cacaccgtgc atggtggtca gcgacaacgg caccgagttc
    13501 acttcacgcg ccatcctggc ttggcaggaa gaacggggcg tggaatggca ctacatcgca
    13561 cccggcaagc caacccagaa cggcttcatc gagagcttca atggccggct acgcgacgag
    13621 tgcctcaacg agcacctctt cgccagcctg ccagaggcca gaaggatcat cgaagaatgg
    13681 aggatcgact acaacacact cagaccgcac acgagcctgg acgggctcac gccgacagag
    13741 tttgcagcac gccccagcca ggggcataac cagaacagac tctccccata aacgggggca
    13801 ttacggggag caggtca
//
LOCUS       DASZYN010000852         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_763472, whole genome shotgun sequence.
ACCESSION   DASZYN010000852 DASZYN010000000
VERSION     DASZYN010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763472"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VHE33_21325"
     CDS             <1..654
                     /locus_tag="VHE33_21325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00054.1"
                     /translation="TNNQLDLRHSSYGPSGTDRDQRFVANFTWQPPRLQLGSTLVRHV
                     LNNWEFSGIGLIQAGTPLSISDTNAGSVYGLIRGEVRAQRTGSNPLTHGSLYQRVING
                     YLDPAAFTRAPEAPNGTSLADQDFGNSGVGFLRGPGQHNVDFAVERLFPIAETHTLHF
                     RTEFFNLTNTPQFANPVNSLGYGDPTDPSPVASSSFGRIFGTVTAPRIVQFALKYQF"
     gene            706..>1623
                     /locus_tag="VHE33_21330"
     CDS             706..>1623
                     /locus_tag="VHE33_21330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00055.1"
                     /translation="MIWTLQQIEEACERSQPPRFERYPTDVPEAALVQTFYPLGFPLE
                     IHTNSEEVLRQCEIKWSIFEKRFAVDPIVAEIFVLDTNETECPPLPQYRFFDDTLVMT
                     ADESNYCIASFPHGKTRIVVTTATLRYPNYFRQVFLDATAACQTSTRHVTGIHSACVA
                     LNHRGVILCGDSGAGKTSLSWACARAGWDFLADDTSYLLRGQDNRVVMGNHHQVRFRP
                     AAATLFPEVAGAEITPRIYGQPSIELPTAPMAHIRTRDCVEVDFIVFLNRRIPGAAEL
                     VPYRKDVARCYMSQWLFGSPEIKSAHQAAI"
BASE COUNT          320 a          595 c          377 g          331 t
ORIGIN      
        1 accaacaatc aactcgacct gcgccactcc tcctacggac cctccggaac cgatcgcgat
       61 cagcgcttcg tcgccaactt tacgtggcag cctccgcgcc tgcagctcgg ctcaacgctc
      121 gtccgccatg tcctcaataa ctgggaattt tccggcatcg gcctcattca ggccggtacg
      181 cctctcagca tctccgatac gaatgccgga tcggtctacg gtctcatccg cggcgaagtc
      241 cgcgcacaac gcaccggcag caatcctctc acgcacggct cgctctacca gcgcgtcatc
      301 aatggatacc tcgacccagc cgctttcacg cgtgcccctg aggcgcccaa cggcaccagc
      361 ctcgctgatc aggacttcgg caacagcggc gtcggctttc tccgcggccc aggccagcac
      421 aacgtcgact tcgccgtcga gcgtctcttc cccatcgctg agactcacac cctccacttc
      481 cgcacggagt tcttcaatct caccaacacc ccgcaattcg ccaatcctgt taacagcctc
      541 ggctacggcg acccaacaga ccccagcccc gtcgccagct catccttcgg ccgtatcttt
      601 ggcacggtca cggctccccg gatcgttcag ttcgcactca aataccagtt ttgaaactca
      661 tggcggccca tcgcgccgcc tcttcccggg agaaaccgca gagaaatgat ttggacactc
      721 caacagatcg aagaagcgtg cgaacgatca cagcccccca ggtttgagcg ctatccgacc
      781 gacgtgcccg aagccgcact ggttcagact ttctacccac tcggattccc tctcgaaatc
      841 cacactaact ccgaagaggt tcttcgtcag tgcgaaatta agtggtcgat ttttgaaaag
      901 cgtttcgccg tcgatcccat cgtcgccgaa atttttgtcc tcgataccaa cgaaaccgag
      961 tgccctcccc ttccccaata ccgcttcttc gacgacacgc tggtgatgac cgccgacgag
     1021 tcgaactatt gcattgcgtc tttccctcat ggcaaaaccc gcatcgtcgt caccaccgcg
     1081 accctgcgct atcccaacta ctttcgtcag gttttcctcg acgccacagc cgcctgccag
     1141 acgagcaccc gtcacgtcac cggaattcac tccgcatgcg tcgccctgaa tcaccgcggc
     1201 gtgatcctct gcggggactc cggcgccggg aaaacttctc tgtcgtgggc ctgcgcccga
     1261 gccggctggg attttctcgc cgatgacacc agctacctgc tgcgcggcca ggacaaccgc
     1321 gtcgtgatgg gcaatcacca ccaggtgcgc tttcgtcccg cggcagccac tctctttcct
     1381 gaggtcgccg gcgcggaaat tacgcctcgc atctacggcc aaccttcgat cgaacttcca
     1441 acggcgccaa tggcccatat ccggacgcgc gattgcgttg aagtcgactt cattgtcttt
     1501 ctcaatcgcc ggatccctgg cgcagccgaa ctcgttccct atcgcaaaga cgtcgctcgc
     1561 tgctacatga gccaatggct tttcggtagt cctgaaataa agtccgccca tcaggcagcc
     1621 atc
//
LOCUS       DASZYN010000853         3270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_638614, whole genome shotgun sequence.
ACCESSION   DASZYN010000853 DASZYN010000000
VERSION     DASZYN010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638614"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3174)
                     /locus_tag="VHE33_21335"
     CDS             complement(<1..3174)
                     /locus_tag="VHE33_21335"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00056.1"
                     /translation="MLFCSLPGCFLLAQETAHEPSSIYSQMQYRFIGPPGNRVTSVLG
                     VPGDSNIYYAGAASGGVWKSWDGGDQWYPIFDREDAQSIGSIALAPSNPNIVWVGTGE
                     PFIRSNVSLGDGIYKSIDGGKTWKHMGLEKTGRIGRILIDPTNPDIVYAAALGTCYGP
                     QQERGVYRTTDGGATWKRVLFVDENTGVYDLTMDPGDSQKIIAASWQIDIKTWGRNSG
                     GPGSGLFMTSDGGDTWTRLTGHGLPPSPLGKIAVSYAPSDGRRVYALIETGQGGTLWR
                     SDDGGDDWTVVNYSRLLNERPHYYTRMLVMPDNENEVYFPSNGISATFDGGETSDHMH
                     CPGDDPAAKAGRERGGCGGDNHDMWSDPKNPARMMIGSDGGVLISTERGRSWHFTRLP
                     IAQMYHVATDNRIPYMVYGQMQDGMPLRGPSDVPGGGEIGAGEWTTTAGCETGWNMPD
                     PVDNNIVWGGCYAGVTERFDAQSGFARSVSVWPDRTMGANAGQVKIRMNWTYPIAISP
                     HDHNTVYVGSQYVHRTTDGGQTWEQISPDLTLNDASMHGDSGGLTVDNLSVEYAGVVY
                     AIAESPVEKGQIWAGTNDGQLQMTRDDGKHWTNLTGNLPGLFPKMTVDSIEPSKYAAG
                     TCYVAFDGHQVDNRDPWLYKTTDYGKTWTKITDGIPASQLSYTHVVREDPVRRGLLYA
                     GTENGLYLSMDDGAHWTSFQQNLPHTPVYWLTIQPEFNDLVVGTYGRGFWILDDISPL
                     QTMKSGLTEEAGHLFPVRHAYRLRAGSRRELAPEGTSVGHNPPPGVPINYLLKSEKSK
                     LTLDISDSQGKVIRHLKPTAHNGINRVWWNLRYEKTIAVALRTTPPGNPHIWSEKRFA
                     GKETRPVFYYGVGGGATDGPLVAPGTYTATLTVDGHAQTQKVVVIKDPRTPATVQDAI
                     AGSALSYRIYEDTNRSAQLINQIEWSRRQIEDTRKLLVAKKSDKSLLDASDALNRKFL
                     DQEDQLLHPTIAEGDEKSFRGPVGLYLKFIWLGAEVGTGGGDVSGNSDFAPTEPEKEV
                     FSLLDKQLDSVAAAVKQIDDRDTADYNA"
BASE COUNT          569 a         1050 c          965 g          686 t
ORIGIN      
        1 cgcgttgtag tcggcggtgt cgcgatcgtc gatctgcttc acggcagcgg cgacggaatc
       61 gagttgcttg tcgagcaggc tgaagacttc cttctccggc tccgtcggcg caaagtcgga
      121 gttcccggaa acatcgccac ccccggttcc cacctcggcg ccgagccaga tgaatttgag
      181 atagaggccc accgggccgc ggaacgactt ttcgtctccc tcggcgatcg tgggatgcag
      241 cagctgatct tcctgatcaa gaaacttccg attcagggcg tcactcgcgt cgagcaggct
      301 cttgtctgat tttttggcga ccagcagttt gcgcgtgtct tcgatctggc gacggctcca
      361 ctcaatctga ttgatcagtt gtgcggagcg gttcgtatcc tcgtagattc ggtaggacag
      421 tgccgacccg gcaatcgcat cctgcacggt cgcaggcgtt cgaggatcct tgatcacgac
      481 gaccttctgc gtttgcgcgt gcccgtcaac cgtgagcgtt gcggtgtaag ttcccggcgc
      541 aaccagcggt ccgtccgttg ctcctccacc gactccgtaa tagaagaccg gccgcgtttc
      601 tttgcctgca aagcgcttct ctgaccagat gtgcgggttc cccggcggtg tggtgcgcaa
      661 agcgacggcg atggttttct cgtagcggag attccaccag acgcgattga tgccgttgtg
      721 tgccgtcggc ttgagatgcc ggataacctt gccctgactg tcgctgatgt cgagcgtgag
      781 tttggatttc tccgacttca gcagatagtt gatcggcact cccggcggag gattgtggcc
      841 gacggaggtt ccctcgggag ccagttccct tcggctgccc gcacgcagcc gatacgcgtg
      901 acgcaccgga aagagatggc ccgcctcttc ggtcagcccg gacttcatcg tctgcagcgg
      961 cgagatatcg tcgaggatcc agaagccgcg gccgtacgtg ccaaccacca gatcattgaa
     1021 ctccggctga atcgtcaacc aatagaccgg tgtgtgtggc aggttttgct ggaacgacgt
     1081 ccagtgcgct ccatcatcca tcgaaaggta gaggccgttc tccgtgcccg catagagaag
     1141 cccgcgccgc accggatctt cacggaccac gtgcgtgtag ctcagttgcg atgcagggat
     1201 cccatcggtg atcttcgtcc aggtcttgcc gtaatccgtc gtcttgtaga gccacggatc
     1261 gcgattgtcg acctgatgcc catcgaacgc cacgtagcag gtgccggccg cgtatttcga
     1321 gggctcgatg ctgtcgaccg tcatcttcgg aaataatccg ggaaggttcc ccgtcaggtt
     1381 cgtccagtgc ttgccgtcat cgcgcgtcat ctgcagctgg ccatcgttgg tgccggccca
     1441 gatctggcct ttttcgacgg gcgattccgc aatcgcgtac acgacgccgg catattccac
     1501 gctgaggttg tccaccgtca gcccgccaga gtcgccatgc atcgaggcgt cgttcagcgt
     1561 gaggtcggga ctgatctgct cccaggtctg tccaccgtcg gtcgtgcgat gcacgtactg
     1621 gctgcccaca tagaccgtgt tgtgatcgtg cggcgaaatc gcaatgggat aggtccagtt
     1681 catgcggatt ttcacctggc ccgcattcgc tcccatcgtg cgatcgggcc acacactcac
     1741 cgaccgcgca aatccgctct gtgcatcgaa gcgctccgtc actcccgcat agcagccgcc
     1801 ccagacaatg ttgttgtcca ccggatcggg catattccat cccgtctcgc aacccgccgt
     1861 tgtcgtccat tcaccggcgc cgatttcccc gccccccggc acgtcgctgg gcccgcgcaa
     1921 gggcatgccg tcctgcatct ggccgtacac catgtacgga atgcgattat cagtggcaac
     1981 gtggtacatc tgcgcgatgg gcagacgagt gaagtgccag gagcgcccgc gttcggtgct
     2041 gatcagcaca ccgccgtcgc tgccaatcat catgcgggcg ggattcttcg ggtcggacca
     2101 catatcgtgg ttatcgccgc cacatccgcc gcgctcgcgg ccggcttttg ccgccggatc
     2161 gtcgccgggg caatgcatgt gatccgaagt ttcaccgcca tcgaacgttg cgctgatgcc
     2221 gttggacggg aagtacacct cattctcgtt gtccggcatc accagcatgc gcgtgtagta
     2281 atgcggacgc tcgttcagca ggcggctgta gttgacgact gtccagtcgt cgccgccatc
     2341 gtcggagcgc cacagcgttc cgccctgccc cgtttcgatc agtgcataca cgcgccgccc
     2401 atcgctcggc gcatagctca ccgcaatctt gcccagcggt gaaggtggca gtccgtgccc
     2461 ggtcagacgc gtccacgtgt cgccgccgtc gctggtcatg aacaggccgc tgccgggacc
     2521 gccgctgttg cgcccccagg ttttgatgtc gatctgccag ctcgccgcaa tgatcttctg
     2581 cgaatcgccg gggtccatcg tcaggtcgta gacgccggtg ttctcatcga caaacaggac
     2641 gcgcttccac gtcgcgccgc catcggtcgt gcggtacacg ccgcgctcct gctgcggccc
     2701 ataacaggtt cccaaagctg cggcgtaaac gatgtcggga ttcgtgggat cgatcaggat
     2761 gcgcccgatg cggcccgtct tctcgagccc catgtgcttc caggttttgc cgccgtcgat
     2821 ggacttatag atcccgtcgc caagcgagac attgctgcgg atgaagggct cgccggtccc
     2881 cacccacacg atgttgggat tcgatggcgc cagcgcgatg gatccgatcg actgcgcatc
     2941 ctcacgatcg aagatcgggt accactggtc tcctccatcc cacgacttcc atactccgcc
     3001 tgaagcggcg ccggcgtagt agatgttgct gtcgccagga acgcccagca ccgacgtgac
     3061 gcgattcccc gggggtccga tgaaccggta ctgcatttgg ctgtagatcg acgacggctc
     3121 gtgtgccgtc tcttgtgcca gaagaaaaca acccggcaaa gagcagaaaa gcatggatcc
     3181 gaccgcccaa cagcccgcgg cgacgcagaa acgaagagcg cgaagtgaca ctcgatcctc
     3241 cgggaaattc ctgccttgcg gagccgattc
//
LOCUS       DASZYN010000854         6572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_890341, whole genome shotgun sequence.
ACCESSION   DASZYN010000854 DASZYN010000000
VERSION     DASZYN010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6572
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890341"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            415..3855
                     /locus_tag="VHE33_21340"
     CDS             415..3855
                     /locus_tag="VHE33_21340"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HVU00057.1"
                     /translation="MSFRFRILIALLAFSAGFSLLHAQSDNSSISGTINDSTGAVLPH
                     ATVTVTNEATHESRTATSNNGGSYTITNLPPGSYTVRVEAQGFETFVQSHNHLEPSIG
                     MQVDASLHVGNAATTVNVVANANVIQTQTAAVGQLVTQQQVQSIQLNGRDPIYLAQLE
                     PGVRRSSSISNFNFGLDNGIQINGANNRESGITWDGAPMVRSRGNGTSIGVADTDSTS
                     EMQVLTAAYPAEYGRSAGGIIRIVPKSGTSDFHGAVFEYLRNSFFNANTWTRKSNTDP
                     NISEHPPAFRFNQFGWNLNGPVWIPGTGFNSGRHKLFFLAGQEWIRYRHVDTDTRKVP
                     SALMRTGDFSELLDPGNLNNFYGKAVQIVNPTTGANYTGNIIPPGDRSANGLGLLNAY
                     PTPNATNPTYNWIDAAIYPENQRKDTLIVDYVPTDRHHLRFSLLNYNYNSVSPHYGNF
                     NRTPQVWDRPNQVGVLHYTWTISPTMVNEAYVSGSADHVSIGIDTSKGLFDRTKYGIN
                     YPFLYPAADKEIPNKIPTIQIANFDTLDGGPYPSHSGGVIWDFSDSLTKVIGAHTIKA
                     GFLYERESQNDFDQINVSSTTPGATNNQNGFFTFTDTRGGNPTSKAAVANTALGLFDT
                     YGEIGVRAYTIFLSNMYAYFAQDTWHARPNLVIEYGIRHEFMLPYYARWGNLSVFSPA
                     TYDAASAPTVDPTTGFVSGSNLYDGIVIPGNGFPSAAKGHVPADILNGQYNNLFHGFS
                     RGYNPTVWTNIQPRLGVTWSITPKTVIRAGGGRYFERVGVSDSVHLGGNPPFQPSATV
                     SYGGVDTPGGTGANNYPINMTSYAYNFPSPETWQWTLTTEHEFTGVGVLTLGYVGTHG
                     LHLQHLENINQLRPGTRQANPGIAKPDALRPFQGFSVIQQETNNGTSNYHSFQANFRH
                     RVSHGLLLGVAYTWSKSLDLASDAGTNIPNYYDPAEFYGPSDFDTRHMLVINYVWDMP
                     FAKNASNWAERNILGRWQLSGTSQFQSGYPLNVSTGDDFAGVGPGSGNQLWVRNGPVE
                     LLKKSSPNNTKNQFWFNPSVFQKPAAGTFAPRESRNQVYGPGFQSWNIAMQKGMPIIP
                     RIESERLIFRAEAFNFVNHPNLDNPQTNPTSNTFGKATGKGNTYASDRQLQFSLRYEF
                     "
     gene            4128..5183
                     /locus_tag="VHE33_21345"
     CDS             4128..5183
                     /locus_tag="VHE33_21345"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HVU00058.1"
                     /translation="MTKRIKAPTLSEVARRAGVGTTTVSRVINGGMRVDPRTLARVRK
                     AIEALGYMPNQAARILKGHRTKTIGLIIPSIADPFFASCAEAAQVIARTHDSLLIVTT
                     THNEPDAEIESVKVLMQHQTDGLIVAPANTDNSALRNLLVRAAVPVVALDRPLLHSSI
                     PFVVADNFAGANLATQHLIGHGYRRIACLTGENTLYTIRERISGYRQAVEAAGLPVIL
                     DDSVTDYPSVELSIRTLLASGAPPDAIFTLKNSITISTFEVMQKLGITIPDSIALLGY
                     DDFELAETLRPSITVVRQPIEEIGRMAAEILFEELQARGARGRVIEHRRTREVQLGTR
                     LVRRRSCGCTPQAPASP"
     gene            5281..>6572
                     /locus_tag="VHE33_21350"
     CDS             5281..>6572
                     /locus_tag="VHE33_21350"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00059.1"
                     /translation="MMRIVPLALLLSIAAVASAQSTPADRPDLKSQPTLYVVGYAHLD
                     TEWRWEYPQVIDEYIPNTMHRNFDLFEKYPHYIFNFSGANRYRFMKEYYPQDFAKVKQ
                     YVAQGRWFPAGSSMEEGDVNAPSAEAIIRQILYGNEWFRREFGTASAEYMLPDCFGFP
                     ASLPTILATSGVKGFSTQKLTWGSSAPGGGVESREKTPEGTPFNVGVWTGPDGESVLA
                     ALNPGSYSGGIYTDLSAPTPPLPPDTAMKTAQDEMRQLQEKFAEAGSKGEKIQPADAQ
                     KFTELRNEMTQLKLDRQNEELSHFQSDWADRVQNNGKVTGVFTDYHYYGTGDIGGSPD
                     EASVKRLEGIVTHGQASMPPPGYVRFNNEAQVNWPDVQVGNGPVHVISAKADQMFLDI
                     TPDERAHLPEYTGEMELTNHSAGSLTSQAYQKRWLRKE"
BASE COUNT         1392 a         2165 c         1765 g         1250 t
ORIGIN      
        1 tgcgaattcc ggctccagca ggtttttccg aagtttttac atctgcctca tgctaagcgg
       61 cgcgatcctg ccgcatagtt actcattgac aatcaaccgc cggactcact atagtgcggt
      121 tcggttgtgg tagcgattcc attttgaacg tgcagggtcg tatttttttc ggaatcaggg
      181 caacgaaaac agaacaaccc gattccgggg aggccgtgca taccgtgctg atggatgctg
      241 tagctgcgcc tgctgtccat ttgccccagc ttgtaagtct ttttcggctg taagtctttt
      301 ctgcgttttt tgaatgtggt atcgattcca tctgtactca gatggaaagg ggaacttgtg
      361 ttgtcaagaa cgggaccccc agaacgaaca ttttgctgct tgaggagacc gcgcatgtcc
      421 ttccgtttca ggatcctgat tgcgttgctg gcgttttccg ccggcttctc gctgctccac
      481 gcgcaatccg ataactcatc gatttcaggc accatcaacg actcaaccgg tgccgtattg
      541 cctcacgcga ccgtcaccgt cacgaatgag gccacccacg agtcccgaac cgcgacttca
      601 aataacggcg gctcctacac gatcaccaat ctgcctccag gcagctacac cgtccgggtc
      661 gaggcgcagg gattcgagac cttcgtccag agtcacaatc atttggaacc gagcatcgga
      721 atgcaggtcg atgcatcgct gcacgtcggc aacgcggcaa ccacggtcaa tgtagttgcc
      781 aatgccaacg tcattcagac ccagaccgcc gccgtcggcc agctcgtgac ccaacagcag
      841 gtgcagtcga ttcagctcaa cggccgtgac cccatctacc tggcgcagct tgagcctggc
      901 gtccgccgca gctcctcgat ctccaacttc aatttcggac tcgacaacgg catccagatt
      961 aacggcgcca acaaccgcga aagcggcatc acctgggacg gcgctcccat ggtgcgcagc
     1021 cgcggcaacg gaaccagcat tggtgtcgcc gatacggatt ccacctccga aatgcaggtg
     1081 ctgaccgccg cgtatccggc tgagtacggc cgttccgccg gaggcatcat ccgcatcgtt
     1141 cccaagagcg ggactagcga ttttcacgga gccgtgttcg agtatctgcg taactctttc
     1201 ttcaacgcga acacctggac ccgcaagtcg aataccgatc caaacatctc cgaacatccg
     1261 cctgctttcc gcttcaatca gttcggatgg aacctcaatg gcccggtctg gattcccggc
     1321 accgggttta acagcggccg tcataagctg ttcttcctcg ccgggcagga gtggatccgc
     1381 tatcgccacg ttgataccga cacgcgcaag gttccatccg cgctgatgcg gaccggcgac
     1441 ttcagcgaac tgctcgatcc gggaaacctg aacaacttct acggaaaagc agtccagatc
     1501 gtgaacccga caacgggggc aaactatacg ggaaatatca ttcctcccgg cgatcgcagc
     1561 gcgaacggcc tcggtctgct caacgcctat ccgacgccga acgccaccaa ccccacctac
     1621 aactggatcg acgccgccat ttaccccgag aatcagcgca aggacaccct gattgtcgac
     1681 tacgtgccga ccgaccggca tcacctgcgc ttctcgctgc tgaattacaa ctacaacagc
     1741 gtttctccgc actacggcaa cttcaaccgc acaccgcagg tgtgggaccg gccgaatcag
     1801 gtgggggttc ttcactacac ctggaccatc agccccacca tggtgaatga agcctacgtg
     1861 tccggaagcg cggatcacgt gagcatcggc atcgacacca gcaaggggct ttttgaccgc
     1921 acgaagtacg gcatcaacta cccctttctg tatccggcag cggataaaga gattcccaac
     1981 aagattccca ccatccagat tgcgaatttc gacaccctcg atggcggtcc gtatccctcc
     2041 cattccggcg gcgtgatctg ggatttctcc gacagcctga ccaaggtcat cggggcgcat
     2101 acgatcaaag ccggcttcct gtacgaacgt gaaagccaga acgactttga ccagatcaac
     2161 gtcagctcca ccactcctgg agccaccaac aaccagaacg gcttcttcac cttcaccgat
     2221 acgcgcggcg gcaatcccac ttcgaaggcc gccgtcgcca acaccgctct cgggctcttc
     2281 gatacctatg gcgaaatcgg cgtgcgcgcg tacacgattt tcctgagcaa catgtacgcc
     2341 tacttcgcgc aggacacgtg gcatgcgcgg cccaacctcg tgatcgagta cggcatccgt
     2401 cacgagttca tgctgcctta ctatgcgcgc tgggggaacc tctcggtctt ctctccggcc
     2461 acctatgacg ccgcctcggc gccgaccgtc gatccgacca ccgggttcgt aagcggctcc
     2521 aacctctatg acggaatcgt gatccccggc aacggctttc ccagcgcggc gaaggggcac
     2581 gttccggccg acatcctgaa cggtcagtac aacaacctgt tccacggatt cagccgcggc
     2641 tacaacccaa ccgtctggac aaacattcag ccacgcctcg gtgtcacctg gtccatcact
     2701 cccaaaaccg tgatccgtgc cggaggcggg cgctatttcg aacgcgtggg tgtcagcgac
     2761 agcgtgcacc tcggcggcaa tccacccttc cagccctcgg ccaccgtcag ctacggcggt
     2821 gtcgatacgc ctgggggcac tggcgccaac aactacccca tcaacatgac ctcgtacgcc
     2881 tacaatttcc cctcgccgga aacctggcag tggacgctga ccaccgaaca tgaattcacc
     2941 ggggtgggcg tgctgacgct gggctacgtc ggaactcacg ggcttcacct gcaacacctg
     3001 gagaacatca atcagctgcg tccgggcacg aggcaggcga atcccgggat cgccaagccc
     3061 gatgcgctgc gtcctttcca gggcttctcc gtgatccagc aggagacgaa caacggtacg
     3121 tccaactatc acagcttcca ggccaacttc agacaccgcg tcagccacgg cctgttgctg
     3181 ggtgttgcct acacctggtc gaagagcctc gatctggcgt ccgatgccgg gacgaacatc
     3241 cccaactact acgatccggc tgagttctac gggcccagcg acttcgacac gcgtcacatg
     3301 ctggtcatca attacgtgtg ggacatgccc tttgccaaaa acgcttccaa ctgggcggaa
     3361 cgcaacatcc tgggccgctg gcagctctcc ggtacaagcc agttccagag cggatatccg
     3421 ctgaacgtat ctaccggcga tgactttgcc ggcgtcggtc ctggatccgg caatcagctt
     3481 tgggtgcgga atggcccggt tgaattgctg aagaagagct cacccaacaa tacgaagaat
     3541 cagttctggt tcaaccccag cgtgttccaa aagccggcgg cgggaacctt cgccccgcgg
     3601 gaaagccgca accaggttta tggtcctggc ttccagagct ggaatatcgc catgcaaaag
     3661 gggatgccga tcattccgag aattgaaagt gagcgtctca tcttccgagc cgaggccttc
     3721 aatttcgtca accatcccaa cctcgacaat cctcagacca acccaaccag caatacattc
     3781 ggaaaagcca ccggcaaggg caatacctac gcctcggacc gtcagctgca gttcagtctg
     3841 cgctacgagt tctgatcgaa caggcatccc ggccccggac gcaggcagca agattctgct
     3901 tgcgcccggg gctgccggtg gctacgctca ttggcaatcg tcttacacaa ctcatcgcgc
     3961 tccactcact cgttccgggc ggttcgatgc acgtgatcca gcgttgaagg acgagtctcg
     4021 cagcgattgg catccgtctg tcctgtgaca gcgaccacac atgcgagatg gaaccctggg
     4081 ctcgccgctg gacgttgctg gcagaatcct ggagtcacgt tgaaggaatg acaaaaagaa
     4141 tcaaggcgcc cacgctgagc gaagtcgcca ggcgggccgg agtcggaacc accaccgtct
     4201 cccgcgtcat caatggcggg atgcgcgtcg atcccaggac tctggcgcgc gtgcgcaagg
     4261 ccatcgaagc tctggggtac atgcccaatc aggcggcgcg catcctcaaa ggccaccgca
     4321 ccaaaaccat cggcctgatt attcccagca tcgccgatcc ttttttcgcc agctgcgccg
     4381 aggcggctca ggtcatcgcc cgcacccatg actctctgct catcgtcacc accacgcata
     4441 atgagccgga cgccgaaatc gaaagcgtca aagtcctcat gcagcatcag accgacggtc
     4501 tgatcgtcgc cccggccaat accgataaca gcgcgctgcg caatctgctg gtgcgcgccg
     4561 ccgtgccggt ggtagcgctc gatcggcccc tgctgcattc ttcgattccg tttgtggtgg
     4621 cggacaactt tgccggtgcg aacctcgcca cccagcacct gatcggtcac ggctaccggc
     4681 gcatcgcctg cctcaccggg gaaaacaccc tgtacacgat ccgcgaacgc atcagcggtt
     4741 atcggcaggc ggtcgaagcc gcagggcttc ccgttatcct cgacgattcg gttacggatt
     4801 atccctccgt cgagctttcc atccgtacgt tgctggcctc cggggctcct cccgacgcca
     4861 tcttcacgct gaagaacagc atcaccatca gcaccttcga ggtcatgcag aagctgggca
     4921 tcacgatccc ggattccatc gcgctgctcg gctacgacga cttcgaactt gccgaaaccc
     4981 tccgcccgtc catcaccgtt gtccgccagc ccatcgagga gatcggtcgc atggctgctg
     5041 aaatcctgtt cgaggaactg caggcgcgcg gtgcccgggg ccgcgtgatc gagcaccgcc
     5101 ggacgcgcga ggttcagctc ggcacccggc tcgtccgccg cagatcctgc gggtgcacac
     5161 cacaggcgcc tgcttcgcct tgacatggga agcaggcccc ttttacgctg gcggctgcgt
     5221 cgtggaaacg ttaccaccca ccttctccct catcctcact gttcatcgcc ggagctcaac
     5281 atgatgcgaa ttgtccccct cgccctcctt ctttccatcg cagcggttgc gagcgcacag
     5341 agcacgcctg cggacagacc ggatctgaaa agccagccaa ccctctatgt ggtgggctac
     5401 gcccatctcg atacggagtg gcggtgggag tatccgcagg tcatcgacga gtacatcccg
     5461 aacacgatgc accgcaattt cgatctcttt gagaagtatc cccactacat tttcaatttc
     5521 agcggcgcga atcgctatcg cttcatgaag gagtactacc ctcaggactt cgcgaaagtg
     5581 aagcagtatg tcgcccaggg ccgctggttt cccgccggct cgtcgatgga agaaggcgac
     5641 gtgaacgcac ccagcgccga ggccatcatt cgtcagatcc tctacgggaa cgaatggttc
     5701 cgccgcgagt ttggaaccgc cagcgccgag tacatgctcc ccgactgctt cgggttcccc
     5761 gcgtctctgc cgacgatcct tgccacctcc ggcgtgaaag gcttctcgac gcaaaagctg
     5821 acctggggct cgtcggctcc cggtggaggc gtcgaatcgc gtgagaagac ccccgagggc
     5881 acgccgttca acgtgggcgt ctggaccgga ccggatggcg agagcgtgct ggcggcactc
     5941 aatccgggtt cgtattccgg cgggatctac accgacctga gcgccccaac gccgcctctg
     6001 ccgcctgaca ccgccatgaa gaccgcacag gacgagatgc ggcagctcca ggaaaaattc
     6061 gcagaagcag gatccaaagg cgagaaaatt cagcctgccg atgcccagaa attcaccgag
     6121 ctgaggaatg aaatgactca gctcaagctc gaccggcaga acgaggagtt gagccacttc
     6181 cagtccgact gggccgatcg cgtgcagaac aacgggaaag tcaccggagt cttcacggat
     6241 tatcactact acggcaccgg cgacatcggc ggctcccccg atgaggcgtc ggtgaagcgg
     6301 ctcgaaggca tcgtgaccca cggccaggcc agcatgccgc ctccaggcta tgtacgcttt
     6361 aacaacgagg cgcaggtcaa ctggcccgac gtccaggtag gcaacggtcc ggttcacgtg
     6421 atttcggcca aggccgatca gatgtttctt gacatcactc cggacgagcg ggcgcacctg
     6481 cccgagtaca ccggcgagat ggagctgacc aaccattccg caggctcgct cacttcgcag
     6541 gcctaccaga aacgctggct gcgcaaggaa ga
//
LOCUS       DASZYN010000855        10053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_765408, whole genome shotgun sequence.
ACCESSION   DASZYN010000855 DASZYN010000000
VERSION     DASZYN010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 10053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10053
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765408"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VHE33_21355"
     CDS             <1..962
                     /locus_tag="VHE33_21355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00060.1"
                     /translation="SLAVTVFVLCSVSLRAQTLREFLTEYKIPTSSVPGPALSQHIVP
                     GAIAQSSRYAFVSWSLPGAALRTWPLHLVRMNRQTRATKTGELRLKTDDVCAGPLVDI
                     RLVEDFVLVATDINPSAQCLLVLDPSLRLRQTLYGFEPQEVEPGRIVMIESMIHFAAV
                     HPERLQFVDLATGQTSELYPPKDDALRAELAKRNEAGMPAHAICEQNNDPCDPKLFDE
                     TLGPFATNGRGRFAMLADQEADHVTASGEDQTIARQSVVYLYQRAGAGWNWCESRLTP
                     VEANQFKGPGSHFAFDQVEGRCQPTRPVVPDETGAATNQFSKP"
     gene            complement(985..2244)
                     /locus_tag="VHE33_21360"
     CDS             complement(985..2244)
                     /locus_tag="VHE33_21360"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HVU00061.1"
                     /translation="MKRTSGKQIQSTTVRAHLAQSQPADFRPGSPHPDADLLTAFAED
                     TLLDRERADVLSHLAACPDCRAVVHTAAPLQPDRESELVPELLHNRHPQLEPLPQPLA
                     AHAATPATAPEPRPTQSPFRRWLPGVALAASLLVMSATTILFYRALHVNPANHTQTAA
                     TTPAAQLPIAPQTAASKPSAGADKKSVPAAARTAKHKEARTPAPSALPTEEARVGAAA
                     PPPPAPPAGISSSVSLGDLSPTAQNVPPPEPAQLQAELRANRDTYQSAMARAKVAPRT
                     TASSSTQSVQVQAGDALTRSLETTHPVAGFIAPPTARPHFRITGNGQLERSGQPNIWV
                     PVSVSPGTHFRVVSISGNDVWAGGDHLRLFHSTDNGVNWSEVHLPAAADRTRAIVHIH
                     IDAPQHLTVEDDSGATWTTSDAGATWQ"
     gene            complement(2241..3176)
                     /locus_tag="VHE33_21365"
     CDS             complement(2241..3176)
                     /locus_tag="VHE33_21365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HVU00062.1"
                     /translation="MSGSVIPTSETSTFPEPAALPPAVSKAISALYSDSGAPRYAISP
                     DQFLQFVTAVVTKYAAAAPQSEQLLLLDKLHLEELILARACSLGNDAAWDTFLNCFRA
                     SLYESAYRIAGNDATGRELADELYADLYGIPNPNGRRISKLDYYMGRGSLEGWLRTVL
                     AQHHIDRCRTYSRTVSLEEQIESGASFPSPADPVTNLPDPRLTSALDKTFSELSPDDR
                     FLLASYYLDQRTLADLARVLRVHESTVSRRLDRITTQLHKRVLKRLRTSGLSGHQCDE
                     LLDELDVRDLSLDLNTKLRQDSASTAFYNKDGSGL"
     gene            3214..4386
                     /locus_tag="VHE33_21370"
     CDS             3214..4386
                     /locus_tag="VHE33_21370"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6569 family protein"
                     /protein_id="HVU00063.1"
                     /translation="MRSSDAEAALKLPQAEADMKVAPECVLGKGHDRWWLAGGASLLL
                     GMAGLGMGGPGGGGPVGSEGTYRVLPAIRRGNLAIFPVVGGKSYNTSQLLTLDEGIRT
                     GEVVVTEAGSDRGLVRGEHPAPSRGSAEVNRLVLYNNSGRPLLLLAGEIVTGGKQDRV
                     IGADRIVPPKGGPIDLSVFCVEPGRWVASSAKFGSMAAQMAQPSVRMPAMAKQNQSEV
                     WDRVRASNAAMANSLSASESHGDVAHTSSYATVFASPPVQAKLEEYGGNAGEKAILEE
                     LRKQGSVGVVVAVNGNVQWADVFASTDLLAKYWPKLMRSYVAEAVTAGSGEGTSTVGE
                     AQSWITKLSGGREVVETEPGVFRRTETTGDGYKVFTLRGLGFGADFEVHMTKMVTG"
     gene            4675..7737
                     /locus_tag="VHE33_21375"
     CDS             4675..7737
                     /locus_tag="VHE33_21375"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HVU00064.1"
                     /translation="MSDSTGAEAKSEATCMVTETWPQALPYTVIFEELRQRRGVEFTQ
                     LVQAWATPGSASVKVPVEEAERRLAEALKDVAVAYANLKWKRWHLRDAARKKYESPAM
                     REAAVETAHQEVEDAAQACADQDAPLKNLLHSCKASALAFSGGGIRSASFSLGVLQRL
                     AQFSVEHGDADHGYLQKLDYLSTVSGGGYTGSWLMSWSRRSSFRSAVEQLGKPAKTAG
                     DPEADPLRHLRSYTSFLAPKYGFSVDTATLAAIVIRNLFLNWVIFLPCLIVLILAPRL
                     LAIAANWLAASVIELPGAADQTLMWVAAGLIAVAGGIAAYRMWEPPVTSAIEKTARLR
                     SETVFMVTTCVAAWLIVDAWLAGWENDPVSYGHIYGWRLLWLVIDLFAASLPLTFVRL
                     KRSHVEPTSTFRTSRNRILWGSVLWSAGAPLTATCATAVALETIAVYVGPRLYGDTDA
                     GNPAFHILAFPVVLSILMLGSGLLSGLLSAVEYEEEREWWARAGGVFLLVMGGWMIAG
                     IVGWYSSFLVTSHYHFTLAWSAGTLVFGWSAAAAGNSASSPVAPKQVDVTQMSGIGRF
                     LLRNQLLAPALSIAALACILLLVGSVTELILERMTAMGSMQALYAAYKSAAPGRTVTI
                     FADPREMCMPILAGVAAIVAGLANYFINLNTFSLHGMYRMRLVRAFLGASNFGRNADR
                     FTNFDPLDNFSEAELGKLRDTPVHVVNTTLNLVDGAKTAWQQRKAESFVFTPFQCGSW
                     RVGYAPSAVYGGLHGVSLGTAMAISGAAFNPNMGYNSSPLVTLVMTLFNARLGWWLPN
                     PGWEKRSTLTEDDQAVYLGKPGPTYSLGSIVLEALGKTNDRRKWVQLSDGGHFENLGL
                     YEMVLRRCHSIVVVDAGADRAFQFEDLGNAVRKINIDLGIPITFSDPWPFSGGKDAGD
                     SYCAIGKIDYCCVDGDAGSPGYLIYIKPRLIGKEPRDVTAYAAGCPAFPHETTVNQFF
                     NEAQFESYRHLGLHEAGAFLGDAKANTQDALLDAALTYCHQERFVTGA"
     gene            complement(7734..7949)
                     /locus_tag="VHE33_21380"
     CDS             complement(7734..7949)
                     /locus_tag="VHE33_21380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00065.1"
                     /translation="MSFPIRTCAVCGEEFELKPDKPGFANRCPACSDPDADDQAGLKT
                     AAGFGNDREANAERRRVMRDMLYRKDS"
     gene            complement(8023..9240)
                     /locus_tag="VHE33_21385"
     CDS             complement(8023..9240)
                     /locus_tag="VHE33_21385"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HVU00066.1"
                     /translation="MIRKTRLFTPGPTPLLPAAQFAMAAADIHHRTPEFRALYERVLS
                     QLKTFVGTQNDVLLLASSGTGAMEASVSNLTSPGDRALVLSAGKFGERWESLAKAFGC
                     QVDLISVPYGETFSLDAIRKAIRPDTRAVFVQATESSTGVRHDIRAIGALLKESGSDA
                     LLIVDAITGLGTTHLDVDAWGVDVIIGGSQKAVMIPPGLAYLSLSDRAWKAAETSKNP
                     RYYFDLRKELKNAKKGESSYTPAVALIAALGAALDYIASQANGDLAAGRDALIENAET
                     CAAMTRAAVQAIGLQLFNSVAPSAAATAILPPAGVDSGVIVKELKRRFAAIVTNGQGE
                     MKGKLFRIAHIGLFDYMDTIAILGALEQVAATSLNLTGFTFGKALAAAQKVYAERTAQ
                     PAAPQNVEALAAV"
     gene            9352..9819
                     /locus_tag="VHE33_21390"
     CDS             9352..9819
                     /locus_tag="VHE33_21390"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HVU00067.1"
                     /translation="MDLAKKIDQDMVAAMKAHDVERTQTLRMVKAALKNRQIEKRSEL
                     TAIEGQQVLTTMIKQRKESIEQFQRGNRPELAAKEAREILLIESYMPKAASEEDLKKM
                     VEEALAGMNFGPRDMGAAMKTVQAKIQETGMRADGRVLSDLVKGKLQGTGSGE"
     gene            complement(9838..>10053)
                     /locus_tag="VHE33_21395"
     CDS             complement(9838..>10053)
                     /locus_tag="VHE33_21395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00068.1"
                     /translation="TKSAKGTGLGLWISKGIIQKYEGSIAFRSLHTPRGACTCFRVFL
                     PLAGDMTVGDTTKEPDISAGAPIRAIA"
BASE COUNT         1839 a         2464 c         3813 g         1937 t
ORIGIN      
        1 atagtctcgc agttacggtt ttcgtgctgt gcagtgtgag ccttcgggcg cagactctgc
       61 gcgagtttct caccgagtac aagattccga cctcgtcggt gccggggccg gctctgagcc
      121 agcacattgt gcccggagcg atcgcacagt cctcccgata tgcctttgtg tcctggtcgc
      181 tgccgggggc tgcattgcgc acgtggccgc tgcatctggt ccggatgaac cggcaaaccc
      241 gcgccacgaa gacaggagag ctgcgactca aaacggacga cgtctgtgcc ggtcctctgg
      301 tcgatatcag gctggtggag gactttgtcc tcgtggccac ggacatcaat ccgtcggcgc
      361 aatgtcttct cgtcctcgat ccttctctgc ggcttcggca gacgctgtat ggcttcgagc
      421 cccaggaggt ggaacctggg cgcattgtga tgatcgagag catgattcac ttcgctgcgg
      481 ttcatcctga gaggttgcag tttgtggatc tcgcgacagg acaaaccagc gaactctatc
      541 cgccgaagga cgatgccctg cgagcggagc tggcgaagcg gaacgaagcc ggcatgcctg
      601 cgcacgcgat ctgcgagcag aacaacgatc cgtgcgatcc gaagctgttc gatgagacgc
      661 tgggcccgtt tgccacgaac gggcgggggc gcttcgcaat gttggccgac caggaggccg
      721 accatgtgac ggccagcgga gaggatcaga cgatcgcccg acagtcggtg gtttatctct
      781 atcaacgcgc cggagcaggg tggaactggt gcgaatcgag gctgacccct gtcgaggcca
      841 atcagttcaa gggtccggga tctcattttg cgttcgatca ggtggagggg cgatgccagc
      901 cgacgcggcc tgttgttccg gatgagacgg gcgctgcgac gaatcagttt tccaagccgt
      961 aggagcgcgg cctgcgctgt tgcgctactg ccaggtggcg ccagcgtccg aggtggtcca
     1021 ggtggcgccg gaatcgtcct cgacggtgag gtgctgcggg gcgtcgatgt ggatgtggac
     1081 gatggcacga gtgcgatcgg cggcggcggg gagatggact tccgaccagt tgacaccgtt
     1141 gtcggtggag tggaacaggc ggagatgatc gccaccggcc cagacatcgt tgccggagat
     1201 ggagacgacg cggaagtggg tgccgggaga aacggaaacc ggcacccaga tgttgggttg
     1261 cccggaacgc tcaagctggc cgtttccggt gatgcggaag tggggacggg ctgttggggg
     1321 agctatgaat cccgcgaccg ggtgggtggt ttcgagggat cgggtcaggg cgtcaccggc
     1381 ctggacctgg acggactggg tggacgatga agcggtggtg cgcggcgcca ctttggctcg
     1441 tgccatcgcg ctctgataag tgtctcgatt cgcgcggagt tcggcctgga gttgtgcagg
     1501 ctcgggcggc ggaacgtttt gcgcggttgg ggagagatcg ccgaggctca cgctggagga
     1561 gatgccggcg ggaggagcgg ggggcggagg agcagcggcg ccgacgcggg cttcttccgt
     1621 tggaagggca gaaggtgcgg gggtgcgcgc ttctttgtgc tttgctgtgc gtgcagctgc
     1681 cggaactgac ttcttgtcgg cgccggctga gggcttggag gctgcggttt gaggcgcgat
     1741 ggggagttgc gcagccggag tggtcgcagc ggtttgcgta tggttcgcgg gattaacgtg
     1801 gagagcgcga taaaagagaa tcgtggttgc ggacatgacg aggagcgaag cggcgagagc
     1861 cacgccagga agccatctgc ggaacggtga ctgcgtgggg cgcggctcag gggcggtggc
     1921 gggagtcgcg gcatgcgcag cgagaggttg aggaagcggc tcgagctgtg gatggcggtt
     1981 gtggaggagt tcaggcacga gctcggattc gcgatccggt tggagaggcg cggcggtgtg
     2041 gacgacggcg cggcagtcgg ggcaggcggc cagatgcgac aaaacgtcgg cgcgctctcg
     2101 gtcgagcaga gtgtcttcgg caaaggcggt gaggagatcc gcgtcggggt gaggactgcc
     2161 tggacgaaaa tcggctggct ggctttgagc caggtgggcg cgcacggtcg tggattgtat
     2221 ctgcttcccg gatgtgcgct tcataggccc gatccatcct tgttatagaa cgcggtagat
     2281 gcggaatctt gccgcagttt tgtgttcagg tcgaggctga gatcgcggac gtcgagttcg
     2341 tcgaggagtt cgtcgcactg gtggccgctg aggccggagg tgcgcaggcg tttgagaacg
     2401 cgcttatgga gctgggtggt gatgcggtcg agacggcggc tgactgtgga ttcgtggacg
     2461 cgcaggacgc gcgcgagatc ggcgagcgtg cgctgatcga ggtagtacga agcgaggagg
     2521 aagcggtcgt cgggggagag ttcgctgaag gttttatcga gagcgctcgt gaggcgcggg
     2581 tcgggcaggt tcgtgacggg atcggcaggg gacgggaagc tggcgccgga ttcaatttgt
     2641 tcttcgaggc tgactgttct cgagtatgtc cggcagcgat cgatgtggtg ctgcgccagc
     2701 acagtgcgga gccagccttc gagggaaccg cggcccatgt agtagtcaag cttcgagatg
     2761 cggcggccgt tgggattggg gatgccgtag aggtcggcat agagttcgtc ggcgagctcg
     2821 cggccggtgg catcgttgcc ggcgatgcga taggcgctct catataagga tgcgcggaaa
     2881 cagttgagga aggtatccca ggcggcgtcg ttaccaagag agcaggcgcg ggcgaggatg
     2941 agctcttcga ggtgaagttt gtcgaggagc aggagctgtt cggattgggg agcggcggcg
     3001 gcgtattttg tcacgacggc ggtgacgaat tggaggaatt ggtccgggga gatagcgtag
     3061 cgcggggcgc cggagtcgga atagagggca gagatggcct tcgacacggc gggagggagt
     3121 gccgccggct cgggaaaggt cgaggtttcg gacgtcggga tcacagaacc agacatgaag
     3181 gcctgaaaaa ctctctgcaa gaacagacac caaatgcgtt cttccgatgc ggaggcagcg
     3241 ctgaagctgc cgcaagcgga ggcagatatg aaggtagcgc cggagtgtgt tttggggaag
     3301 gggcacgatc gctggtggct ggcgggaggt gcatcgctgc tgctcggaat ggctgggtta
     3361 ggtatgggtg ggccgggcgg aggcggcccg gtggggagtg aggggactta tcgggtgctg
     3421 ccggcgatcc ggcgtggcaa tctggcgatt tttccggtgg tgggtggaaa gtcgtataac
     3481 acctcgcagc ttttgacgct cgatgaagga attcgtaccg gcgaggtggt ggtcacggag
     3541 gcagggagcg atcggggcct ggtgcgcggg gagcatccgg ctccgtctcg cggcagcgcg
     3601 gaggtgaatc gcctggtgct ctacaacaat tccggaaggc cgctgttgtt gctggccgga
     3661 gagattgtga cggggggcaa gcaggatcgc gtgatcgggg cggatcgcat cgtgccgccg
     3721 aagggtggac cgatcgatct gagcgtgttc tgcgtggagc cgggacggtg ggtggcgtcg
     3781 agcgcgaagt ttggatcgat ggcggcgcag atggcgcagc cgagcgtgcg catgccggcg
     3841 atggcgaagc agaaccagag cgaagtgtgg gaccgcgtaa gggcatcgaa tgcggcgatg
     3901 gcgaattcac tgtcggcttc cgaatcccat ggagatgtgg cgcacacgag ttcctatgcg
     3961 acggtgtttg cgagcccacc ggtgcaggcg aagctggagg aatacggagg caatgcaggc
     4021 gagaaggcga ttcttgagga actgcggaag cagggctccg tgggtgtggt ggtggcggtg
     4081 aacggcaatg tgcagtgggc agatgtattc gcgagcaccg atctgctggc gaagtactgg
     4141 cccaagctga tgcgaagcta cgtggcggag gcggtgacgg ctggatccgg cgaggggacg
     4201 tcgacagtgg gtgaggcgca gtcctggatc acgaagctga gcggtgggcg ggaagttgtg
     4261 gagacggagc ccggcgtttt tcggcggacg gagacgactg gggacgggta caaggtgttc
     4321 actttgcgtg ggctggggtt cggggcggac tttgaggtgc acatgacgaa gatggtgacc
     4381 gggtgaaggg agaacttggc gtagcggggt tcagcgaaag ggacctcgtt accgcagtgc
     4441 cgacgagatg cattcgcggc gcgcattgtg atgcgatgtt ttcttgtagg gcgcagcgat
     4501 ttctgggtat agttctgcaa cggttcttcc cagccagcca ctttactttt gtgcgaatgg
     4561 ccgtgcgctg attttttccc cagacctgcc gaacctgtgc ggaagagccg gctttcaatc
     4621 agctgtccga ttccattcgt gtgcacgcgc gtgcacgcgt ttccgggagg ggcgatgagc
     4681 gattccaccg gcgcagaggc gaaatccgaa gcgacctgca tggtcaccga gacatggccg
     4741 caggcgctcc cttacacggt gatctttgag gagttgcgcc agcggcgcgg cgtggagttc
     4801 acacagctcg tacaagcgtg ggcgacgccg ggcagcgcgt cggtcaaggt gccggtggaa
     4861 gaggcggagc ggcgcctggc agaagcgctg aaagatgtcg cagtggccta tgcaaacctg
     4921 aagtggaagc gctggcattt gcgagatgct gcgcgaaaga agtacgaatc gccggcgatg
     4981 cgcgaggctg cggtcgagac ggcgcaccag gaagtggagg acgcggcgca ggcgtgcgca
     5041 gaccaggatg cgccgctgaa gaatctgctg catagctgca aagcttcggc gctggcgttt
     5101 tccggaggag gaattcgaag cgcgagcttt tcccttgggg tgttgcagcg actggcgcag
     5161 ttcagcgtcg agcacggtga cgcggaccac ggatatctgc agaagctgga ttatctgtcg
     5221 acggtttcgg gcggcgggta taccggctcg tggctgatga gctggtcgcg tcgcagcagc
     5281 tttcgctcgg ccgtagagca gttgggaaag ccggcgaaaa cggcgggcga tccagaggcg
     5341 gatccgttgc ggcatctgcg cagctatacg agtttcctgg cgccgaagta tgggttctca
     5401 gtcgatacgg cgacgctggc ggcgattgtc atccggaacc tgtttttgaa ttgggtgatc
     5461 tttctgccgt gtctgatcgt tttgatcctg gcgccgcgtt tgctcgcgat tgcggcgaac
     5521 tggctggcgg cttcggtgat tgagttgccg ggagcggccg atcagacgct gatgtgggtg
     5581 gctgccggtt tgattgcggt ggcgggggga attgcggcgt atcgcatgtg ggagccgccc
     5641 gtcacgagcg caatcgagaa gacagcccgc ctgcgcagcg agacggtttt catggtcacg
     5701 acgtgcgtgg cagcgtggct gatcgtcgac gcgtggctgg cgggatggga aaacgaccct
     5761 gtttcttacg ggcacatcta tggctggcga ctgctgtggc tggtgatcga cctgttcgcg
     5821 gcgtcgttgc cgttgacgtt tgtgcgactg aagcggagtc atgtggagcc caccagcacg
     5881 ttccgcacgt cgagaaaccg gattttgtgg ggcagcgtgt tgtggtccgc aggcgcgccg
     5941 ctaacggcaa cgtgcgctac cgcggtggca ctggaaacga ttgcggtgta tgtggggccg
     6001 aggctctacg gagacaccga tgccggaaat ccggcgttcc acatcctggc ttttccggtg
     6061 gtactcagca tcctgatgct ggggagcgga ctgctttccg gtctgctgag cgcggtggag
     6121 tatgaggagg agcgcgaatg gtgggcgcgt gccggcgggg ttttcctgct ggtgatgggc
     6181 ggttggatga tcgcggggat cgtgggctgg tactcgagct tcctggtgac gtcgcactat
     6241 cacttcaccc tggcatggag tgcggggacg ctggtgttcg gatggagcgc ggcggcggcg
     6301 ggcaacagcg cgtcttcgcc cgtagcaccg aaacaggtag atgtgacgca gatgtcaggg
     6361 atcgggcggt tcctgctgcg caatcaactg ctggctccgg cgctgtcgat tgcggctctg
     6421 gcgtgcatcc tgctgctggt gggaagtgtg actgaactca tcctggagcg catgacagcg
     6481 atgggatcga tgcaggcgct ctatgcagcg tacaagagcg cggcgccagg gagaacggtg
     6541 acgatctttg ccgacccgcg tgagatgtgc atgccgatcc ttgccggggt agcggcgatc
     6601 gtcgcggggc tggcgaacta cttcattaac ctgaacacct tctcgctgca tggcatgtat
     6661 cggatgcggc tggtgcgggc gtttttgggc gcatcgaatt ttgggaggaa cgcggatcgc
     6721 tttaccaact tcgacccgct cgacaacttc agtgaagcgg aactggggaa gcttcgtgat
     6781 acgccggtgc atgtggtgaa tacgacactg aatttggtgg acggggcgaa aaccgcgtgg
     6841 cagcagagga aggcggagtc gtttgtgttc acgccctttc aatgcggaag ctggcgcgtg
     6901 ggctatgcgc cgagcgcggt gtatggcggg ctgcacggcg tcagcctggg gacggcgatg
     6961 gcgatcagcg gagcggcctt caatcccaat atggggtaca actcgtcgcc gctggtgacg
     7021 ctggtgatga cgctgttcaa cgcgcgcctg gggtggtggc tgccgaatcc ggggtgggag
     7081 aagcgttcga ctctcaccga ggatgatcag gcagtgtatc tggggaagcc ggggccgacg
     7141 tattcactgg gatcgatcgt gctggaagcc ctggggaaga cgaacgatcg gcggaagtgg
     7201 gttcagctat ccgacggcgg ccatttcgag aatctcggtt tgtacgagat ggtgctgcgg
     7261 cggtgtcact cgattgttgt ggtggatgcg ggggccgatc gggcattcca gtttgaagat
     7321 ctgggcaatg cagtccgcaa gatcaacatc gatctgggaa ttccgattac attcagcgac
     7381 ccctggccgt tttccggagg caaggacgcc ggggacagtt attgcgcgat cgggaagatc
     7441 gactattgct gtgtggatgg ggatgcaggt tctcccgggt atctgatcta catcaagcca
     7501 agactgattg ggaaagagcc gcgcgatgta acggcgtatg ccgcgggatg ccctgcgttt
     7561 ccacatgaga ccacggtgaa tcagttcttc aacgaggcac agtttgagag ctaccggcac
     7621 ctggggctgc acgaggcggg cgcgtttctc ggcgatgcaa aagcaaacac gcaggacgca
     7681 ctgctggatg ccgcgctgac atactgccac caggaacgct tcgtcacggg ggcctagctg
     7741 tctttgcggt agagcatgtc gcgcatcacg cgacggcgtt ccgcgttggc ctcgcggtca
     7801 ttgccaaatc cggcagcggt tttgagcccc gcctgatcgt cggcgtcagg gtcgctgcag
     7861 gcaggacaac gattggcgaa gccgggtttg tcgggcttca gttcgaactc ctcaccacag
     7921 acagcgcagg tacgaatcgg aaacgacatt gcaattcctt gatgctcccg gcgaaaaatc
     7981 gttctttgtg gcggattctt cgccgggatg attccttgaa atttatacgg ccgcgagagc
     8041 ctcgacgttc tgcggggccg cgggctgggc ggtgcgttcg gcgtagacct tttgcgcggc
     8101 ggcaagcgct ttgccaaagg tgaagccggt gaggttgagc gatgtggcgg cgacctgctc
     8161 gagggcaccc aggatggcga tggtgtccat gtagtcgaag aggccgatgt gggcgatgcg
     8221 gaagagcttg cccttcatct ccccctggcc gttcgttacg atggcggcga agcggcgctt
     8281 gagttccttc acgatcacgc cggagtcgac cccggcgggt gggaggatcg cggtggctgc
     8341 ggccgatggc gcaacggagt tgaagagctg gagaccgatg gcctgaacgg cggcgcgggt
     8401 catggcagcg caggtttcgg cgttttcgat gagggcatcg cggccggcgg ccagatcacc
     8461 gttggcctgg gaggcgatgt agtcgagcgc ggcgccgagc gcggcgatga gagcgacggc
     8521 gggcgtatag gaggactcgc ctttcttcgc gttcttgagt tccttgcgga ggtcgaagta
     8581 gtagcgcgga ttcttcgagg tttcggcggc cttccatgcg cgatcgctga ggctgaggta
     8641 ggcaaggccg gggggaatca taacggcttt ttgcgagccg ccgatgatga cgtcgacacc
     8701 ccaggcatcg acatcgagat gggtggtgcc gaggccggtg atggcatcga cgataaggag
     8761 cgcatcgctg ccggattctt tcaggagggc accgatggcc cggatgtcat gacggacacc
     8821 ggtggaggac tcggtggcct gcacgaagac ggcgcgggtg tcgggccgga ttgccttgcg
     8881 gatcgcgtcg agggaaaagg tttcgccata cggaacgctg atgagatcaa cctggcatcc
     8941 gaaggctttg gcgagagatt cccagcgctc gccgaatttg ccggcggaga ggacgagggc
     9001 gcggtcgccg ggcgaggtga ggttcgagac ggaggcttcc atagcgccgg tgccggagga
     9061 ggcgagcagc agcacgtcgt tttgagtgcc gacgaaggtt ttgagctgtg aaaggacgcg
     9121 ctcgtacagg gcgcggaatt cgggagtgcg gtggtggatg tccgctgcgg ccatggcgaa
     9181 ctgagccgcg ggaaggagcg gggtcgggcc gggtgtgaac agacgcgtct tgcgaatcat
     9241 aggatgagag cctctccaga tcattttaaa acgtgggcgc gggaagagtc ggttggattt
     9301 ctccctataa tgaggcgtct gaggacttga ccgaaaagca gggagggtgt cgtggatctg
     9361 gcgaaaaaga tcgaccagga tatggtggcg gcgatgaagg cgcacgatgt cgagcgtacg
     9421 caaacgctgc ggatggtgaa ggcggcgctg aagaaccggc agatcgagaa gcggtcggaa
     9481 ctgacggcta tcgagggtca gcaggttttg accacgatga tcaagcagcg gaaggaatcg
     9541 atcgagcagt ttcagagggg caaccggccg gaactggcgg caaaggaagc gcgagagatt
     9601 ctgctgatcg agagctacat gccgaaggct gcgagcgaag aggatttgaa gaagatggtg
     9661 gaggaagctc ttgcggggat gaattttggg ccacgcgaca tgggcgcggc gatgaagacg
     9721 gtgcaggcga agattcagga gacaggaatg cgggctgacg gaagggtgct gagcgacctg
     9781 gtgaagggga aattacaggg aacagggagc ggggagtagg cagaagaccc aaacctctca
     9841 ggcgatggcg cggatagggg cgccggcgga gatgtcgggc tctttcgttg tatcgccgac
     9901 ggtcatgtcg ccggcgagag gcaggaagac acgaaaacag gtacaggctc cgcgaggggt
     9961 gtgcagactg cggaacgcga tggagccttc gtatttctgg atgatgcctt tactgatcca
    10021 gagaccgagc ccggtgccct tggcggattt ggt
//
LOCUS       DASZYN010000856         6629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_706602, whole genome shotgun sequence.
ACCESSION   DASZYN010000856 DASZYN010000000
VERSION     DASZYN010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706602"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VHE33_21400"
     CDS             complement(<1..551)
                     /locus_tag="VHE33_21400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00069.1"
                     /translation="MKPMGETALQDHGSAWTLERQAAFIAEIGVETLLFCPDGWALRH
                     RFINTRTGETVRARCGSWSCLYCGPRKVDQWRQLVKAAEPTLFITLTKVGTTLKEASR
                     TLTTVLQYLRRGSKGRGKLHVGARPAYPIQVFAVLEEHSDFEENGFHWHLLVKGVDFI
                     PNQVVSDALRSATGGRSYITNVKR"
     gene            complement(567..890)
                     /locus_tag="VHE33_21405"
     CDS             complement(567..890)
                     /locus_tag="VHE33_21405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00070.1"
                     /translation="METVKQVELWPGLWPGVAAIVGDAELLCIACAEYRFGVASVRRV
                     IDGGAEQGEQTDQWGHTLGVVLCGSKDLHGTYCGRCQRPLCDEDCICYQPGQADHWNQ
                     YGTFL"
     gene            complement(1605..1811)
                     /locus_tag="VHE33_21410"
     CDS             complement(1605..1811)
                     /locus_tag="VHE33_21410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00071.1"
                     /translation="MQDGAGVVMRVQGKRANRMYLRGWVVWWPGARLVRERWLSDAYW
                     VSGDVQTFLLAAEAAGCRAEMMHS"
     gene            complement(1834..2094)
                     /locus_tag="VHE33_21415"
     CDS             complement(1834..2094)
                     /locus_tag="VHE33_21415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00072.1"
                     /translation="MEHDEPWRRFDVAIWRRWPPGSDALPVLGVVTAWDAAEAVEAVM
                     RVYGIQAAGHVAACELGRRFVHRAYGVRLREEGAFVRRAGNG"
     gene            complement(2164..3483)
                     /locus_tag="VHE33_21420"
     CDS             complement(2164..3483)
                     /locus_tag="VHE33_21420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00073.1"
                     /translation="MDYTTIKQLSKRMGVRVTDLIALAPQNDPFYTGTPNDWVLAEWF
                     AALWRAFGYGEGVHLRRIHYQIISQDPPMALPNGELYENTMECWDVLNLASKAARYLG
                     LVSAAAFVDRRNADAVEYAEYKLSTPEARVWDYLSRYDFRFPDFPSLPRYTVTGYEAS
                     QGYHLEIWCEKSTMNDILLPLCQEYGANLQIGSGELSITAALSLVGRLERAGRPARIF
                     YVSDFDPAGQSMPVAMSRKVEYFVQTLGLDLDIRVFPVVLTLDQVREYGLPRTPIKET
                     ERRRAGFEARHGEGAVELDALEALYPGCLASILRAHMDAYYDHTLEDRVRQVEEQLRA
                     DLAEREDGVLGAYEPDIEALKQEHEVLKQQYAEQMEGYMQRLRHLWQAIGADLRANLL
                     DARDYPVPLPSVVEDLDEGLYNSRRDYLEQIAVYKEFQGKGTELVTM"
     gene            complement(3556..4230)
                     /locus_tag="VHE33_21425"
     CDS             complement(3556..4230)
                     /locus_tag="VHE33_21425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00074.1"
                     /translation="MMRRMFTGGSWIRVFEHTILRIVSLAFSLASAYAIRVFFEPLDA
                     ADPGERVITWIVAAGFGVLGYVVSRGLVHRMMQKESIWAYAPICFLVELVDISCNYVL
                     AASVVREAWWLNAVPENQRGILTVITYVVLSAIPLVSLFLAVVDMDMERSKHGAAAPV
                     RANKPVTPLRSAPPMRPVTPPGNPSATGAAGAARRAQFWNKQQQGGGNPMSYDPDKEL
                     AGAGEM"
     gene            complement(4247..4537)
                     /locus_tag="VHE33_21430"
     CDS             complement(4247..4537)
                     /locus_tag="VHE33_21430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00075.1"
                     /translation="MSAKINDFFGMEANIFDIDEEQTYKAEIVGGVDGLAELVQLKKA
                     SMKKGGVSDADLEAAASQVQEPPKMQPLPIRVLRVKANKQGFLTLVCELSQQ"
     gene            complement(4828..5037)
                     /locus_tag="VHE33_21435"
     CDS             complement(4828..5037)
                     /locus_tag="VHE33_21435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00076.1"
                     /translation="MENIAFQLQGDQLVIVVDLSHEAGESASGKSIIIATTGGNVSVP
                     GHEDIKIGMNVYRPIQAARPVRRSR"
     gene            complement(5084..5521)
                     /locus_tag="VHE33_21440"
     CDS             complement(5084..5521)
                     /locus_tag="VHE33_21440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00077.1"
                     /translation="MMPANIECIEEGDIGGWISPDGSFFQAHYCEHEDVARQIIGKWL
                     GEAYEGYWPGDYLVRRGWIRLETTGRIWHDFRPTPQQADMLYRCYVRGSDDYQGELYP
                     CIPTIVQEAVRVRESVWSVPAQGDQENMNIRRMFAGRGISLYR"
     gene            complement(5540..5716)
                     /locus_tag="VHE33_21445"
     CDS             complement(5540..5716)
                     /locus_tag="VHE33_21445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00078.1"
                     /translation="MGTPVLIFAVSFVLTWVALRVRSKGVFIGGMVVLVGLVTLHCLI
                     ASGVVVWPFTWLAI"
     gene            complement(5882..6199)
                     /locus_tag="VHE33_21450"
     CDS             complement(5882..6199)
                     /locus_tag="VHE33_21450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00079.1"
                     /translation="MAGMEREFENNMVELVERCPWMTDEDQWMMRRVLASHFGLAFDA
                     WETVDLGQMAEDVQMLASRAEEEGDQRRAWAFTEWYNWVCAALRGEEYQGPEMPEELA
                     ALP"
BASE COUNT         1415 a         2441 c         1503 g         1270 t
ORIGIN      
        1 cgcttgacgt tggtgatata cgagcgtccg cccgtggccg agcgcaacgc atcactcacc
       61 acttgattcg ggatgaagtc cacgcctttc accagcaaat gccagtgaaa cccattctcc
      121 tcaaagtcgc tatgctcctc cagcaccgcg aatacttgaa tgggataggc tggacgcgcc
      181 cccacatgca acttcccacg ccctttcgac ccacgccgca ggtattgcag taccgtcgtc
      241 agcgtccgtg acgcctcttt gagtgtggtc cctactttcg tcagcgtgat aaacagcgtc
      301 ggttctgctg ccttgaccag ttgccgccac tgatcgacct tacgcggtcc acagtacagg
      361 cacgaccagg aaccgcaccg cgcccgtact gtctcccctg tcctggtgtt gataaagcga
      421 tgccgcaatg cccacccatc gggacaaaac agcagcgtct ctacccctat ctctgctata
      481 aatgccgcct gtcgttccag cgtccacgcc gaaccatgat cctgtaaagc tgtttccccc
      541 atcggcttca cctcgctttc tccgatctac aagaacgtcc cgtactggtt ccaatggtct
      601 gcctgtccgg gctggtagca gatgcagtcc tcatcacaca gcggacgctg acagcgcccg
      661 cagtatgttc catgcagatc tttcgagcca cataggacca caccgagcgt atggccccac
      721 tgatccgttt gctctccctg ctctgctcca ccatcgatca ctcgccgaac cgaagcaacg
      781 ccaaagcgat attccgcaca cgcgatgcac aacaattccg catcacccac aatggccgca
      841 acgcccggcc acaatcctgg ccagagttca acctgtttca ctgtctccat agacaccacc
      901 tccttgtttc caacggcgtt tgagacgccc gtacttgtgt gacttgttct tagaagagtc
      961 aagtaaagac tctggcggcg atgcggcggg cgcggacagg tccggcccgc cgcccctgcg
     1021 ttgcgggcac cgctgcaccc caggccagac cacaccacac gccaccccgc aaggaaccgc
     1081 tacgcggata gaaagggcgg gtcagcaccg cgaagcgggt cagcggccag caccgccggg
     1141 tgatgccgcc gttgacagcg ggggctgagc gctgcgcgcc gagagtgacc gggttctgca
     1201 ttgatgcgtt ggttcgtagg cggtcactct cgctctgcga gtctagcccc cgctgtcaac
     1261 ggttccagag cggcatcact tgctccggcg gtactggcgg gcgagcaggt cagcaccgcg
     1321 aggaggcctc aagttcccga gatgcttgat gagccatccg gctcccgccg ctccgcttcc
     1381 cgctcaccct ccgccctggc ctgctcctcc agaaacgccg acaacgccgc atctcccgcc
     1441 caatacgcgc gacaatccgg gcaagggcag tcatctgcat gaagtcccca actctggaac
     1501 cgtgttgacc atttcacccc ctcggcatag tcccaacacc cgaggctctc ataccagacc
     1561 ttttcccatg ttctcttcat cggcctcacc atcctttcca atggctagct atgcatcatt
     1621 tccgctcgac atcccgccgc ctcagccgcc agcagaaatg tttggacatc acccgatacc
     1681 caatacgcat cactcaacca gcgttcgcgc accagccgcg cacctggcca ccagacaacc
     1741 catcctcgca ggtacatgcg gttcgcccgc ttgccctgta cccgcatcac gacacccgca
     1801 ccatcttgca tcggtatctc ctttctcaca tggctagcca ttgccggccc gccgcacaaa
     1861 cgcgccctct tcccgtagcc gcaccccata cgcccgatgc acaaaccgcc gccccagttc
     1921 gcacgctgct acatgacccg ctgcctgtat cccatacacc ctcatcaccg cttccactgc
     1981 ctccgctgcg tcccaggccg tgacgacccc caggactggc aacgcgtccg accccggagg
     2041 ccagcgccgc cagatcgcta catcaaagcg ccgccacggt tcgtcatgct ccataggctt
     2101 ctctcctttc cgcactgaca acaagacgcc cgtatgaccg ttcatacgag cgcctcgcct
     2161 gctctacatc gtgaccagtt ctgttccctt gccctggaac tccttgtaga ccgcaatttg
     2221 ctccaggtag tcccgccgac tgttgtacaa cccctcatcc agatcctcca ccaccgaggg
     2281 cagcggcacc ggatagtccc gcgcatccag caggttcgcc cgcagatccg cgccaatggc
     2341 ttgccagagg tgccgaagcc gctgcatgta cccctccatc tgctcagcgt actgttgctt
     2401 gagcacctca tgctcctgct tgagcgcctc aatatctggt tcatacgccc caagcacccc
     2461 gtcctcccgt tcggccagat ctgcccgcaa ctgctcctct acctggcgca cccggtcctc
     2521 cagggtatgg tcatagtagg catccatatg cgcccgcagg atggaggcca gacaccccgg
     2581 atacagcgcc tctaaggcat ccagttcgac cgcgccctcg ccatgccgcg cctcgaagcc
     2641 agcacgccgc cgctccgtct ctttgatcgg tgtgcgcggc agcccatact cgcgcacctg
     2701 atcgagcgtg agcaccaccg gaaacacgcg aatatccaga tccaggccca gcgtttgtac
     2761 aaagtactcc accttgcgcg acatggccac cggcatggac tggcccgccg ggtcaaagtc
     2821 cgagacatag aaaatgcgcg cgggacgccc ggcccgctcc agacggccca ccagtgaaag
     2881 cgccgccgtg atggagagtt ccccggaacc gatctgcaaa ttcgccccgt attcctggca
     2941 gagcggcagc aggatatcat tcatggtgga cttctcgcac cagatttcca ggtgataacc
     3001 ctgactggcc tcataacccg tcacggtata gcgcggcaac gatgggaagt cgggaaaccg
     3061 gaaatcatag cggctcaggt agtcccacac ccgcgcctct ggcgtactca gcttgtactc
     3121 ggcgtattcc accgcatcgg cattgcgccg atccacgaac gccgccgcgc tcaccagccc
     3181 caggtagcgc gccgccttag acgccagatt gagcacatcc caacactcca tcgtgttctc
     3241 atacagctca ccattcggca gtgccatcgg tggatcctgg ctgatgatct ggtagtgaat
     3301 acgccgcaga tgcacgccct ctccatagcc aaaggcccgc cacagcgccg cgaaccattc
     3361 cgccagcacc caatcgttgg gggtgcccgt atagaacggg tcattttgcg gagccagcgc
     3421 gatcaggtcc gtcacccgca cgcccatgcg cttgcttaat tgtttgatcg tggtatagtc
     3481 cataggcctc accatccttt ctgtgaaacc gggtgcctgc cactcaccag agccacaagc
     3541 accctctctc catgcttaca tctcacccgc gcccgccagt tccttgtccg ggtcatagct
     3601 catcgggttg ccgccgccct gctgctgctt gttccagaac tgcgcccgcc gcgccgcgcc
     3661 cgccgcaccg gttgcactcg ggttccctgg cggtgtgacc ggcctcatcg ggggagcgga
     3721 cctgagcggt gtcactggct tgttcgccct gactggagcc gccgccccat gttttgagcg
     3781 ctccatatcc atatccacca ccgccaggaa cagcgacacc agcggaatgg ccgacagcac
     3841 cacgtaggta atcaccgtca agattccgcg ctggttctcc ggcaccgcat tgagccacca
     3901 cgcctcacgc accaccgacg ccgccagcac atagttacag gagatgtcca ccagctccac
     3961 caggaagcaa atcggcgcat aggcccagat gctctctttt tgcatcatgc gatgcaccag
     4021 cccgcgcgac accacatagc ccaggacgcc gaagcccgcc gccacaatcc aggtaatcac
     4081 ccgctccccc ggatcagccg catctaacgg ctcgaaaaac actcggatcg cgtaggcgct
     4141 ggccagcgag aaggccaggg acacaatgcg caaaatggta tgctcgaata cccgtatcca
     4201 tgacccgccc gtaaacatac gtctcatcat aggcctctac ccctttctat tgctggctca
     4261 actcacacac cagcgtcaga aacccctgct tattggcttt gacgcgcagg acacgaatcg
     4321 gcaagggttg catcttcgga ggttcctgca cctgggaagc cgccgcctcc aggtccgcat
     4381 cagacacgcc acctttcttc atggaggcct tcttcaactg caccagttcc gccaatccgt
     4441 ccacgccccc gacaatctcc gccttatacg tctgctcctc gtcaatgtcg aagatgttgg
     4501 cttccatgcc gaagaaatca ttaatcttcg cgctcataat cgttccgttc ataaacatag
     4561 gcctcaccat tcctttctct gcccgcaggc atcttaccgg gcgctctcat ccgtcgccca
     4621 cacgatccgc tcagctaatc cattgagtgc gacaaaccgc tgatgcttgc cgcatagatg
     4681 cacttttgag gtatccggca cctccacaca cccgggacac ccggccacca gcccgcagcc
     4741 ctggcacaca aaccatccga agacgaccgc gtgtcccatc tgatgtttca ggccgcactt
     4801 tccacgacga ccacccacac gcaccaccta gcgagaccgg cgaaccggac gcgccgcctg
     4861 tataggacga tagacattca tcccaatctt gatatcctca tgaccaggaa ccgagacatt
     4921 gccgcccgtc gtggcgatga tgatcgactt gccgctggct gactccccag cctcatggct
     4981 cagatcgacg acgatcacca gttgatctcc ttgcagttga aacgcaatat tctccataga
     5041 caacaccatc ctttcttcat atggcagcca tgcaacgcct gagctacctg taaagactaa
     5101 tccccctacc agcaaacatc cgacgaatgt tcatattctc ctgatcccct tgcgctggaa
     5161 ccgaccaaac agactcacgc acgcgcaccg cctcctgaac aatcgtggga atacaaggat
     5221 aaagctcccc ctgatagtca tcagaaccac gtacataaca tctatacaac atgtcagcct
     5281 gttgtggcgt tggccggaag tcatgccaga tacgtccagt tgtttccaac ctgatccagc
     5341 cacgtcgtac cagataatca cccggccagt atccctcgta agcttcccct aaccacttcc
     5401 cgataatctg ccgcgccaca tcctcatgct cacaataatg cgcctggaag aacgacccat
     5461 caggagaaat ccaccctccg atatcgccct cctcaataca ctcgatattc gcaggcatca
     5521 taggcatacc atcctttcct catatggcta gccatgtgaa aggccagacc acaacgcccg
     5581 aagcaatcag acaatgcagc gtcaccagtc ccaccagcac caccattccg ccaataaaca
     5641 cccccttgct cctcaccctc agcgccaccc acgtcaggac aaacgacacg gcgaaaatca
     5701 gaactggcgt tcccatacgc cctgctcctt tccaatcaac ttgacagtca gactcccatg
     5761 tgagaagctc tctgtgagaa cctgaccgcc gctacccaaa cgcgctccgc atgttctccg
     5821 tcctggccga tcaggaccag tgaccagcca ggacacccga tgtattccct taccaccatc
     5881 ttcatggcag cgccgccaac tcctccggca tctccggccc ctggtactct tccccacgta
     5941 atgccgcaca cacccagttg taccactccg taaacgccca tgcccgccgc tgatcaccct
     6001 cctcttccgc ccggcttgcc agcatctgta catcctctgc catctgcccc aggtccacag
     6061 tctcccacgc atcaaacgcc aacccaaaat gacttgccag aacacgccgc atcatccact
     6121 gatcctcatc ggtcatccag ggacaccgtt ccaccaattc caccatattg ttctcgaatt
     6181 ccctttccat cccggccata caccgttcat acatacgctc aggcatacac ttctatcccc
     6241 tcctccgctg ccagcaattc accccaacac cagccgcagc gattacccag agacgcggga
     6301 tgattcatcg gaagcacccg caggcaatcc gagcactgaa gaacgcgcaa cggcacccgc
     6361 gcatgttgcg gacgtttcgg gtagcgctcc gcttgacgct gttctgcttt gtttccccat
     6421 cgctgaaacc gatccatagg cacaccatcc tttctgtgat taccgacacc gtggacaccg
     6481 aagacgacaa cttacgcata acaccgcccc acaccgacga cagacacaga ccgactcagg
     6541 aagcaaccgg gcgaagcacc ggagacacga caggcgctga accagacctc tcaccatcgc
     6601 tcacccctta acccaggccc ggcaacagc
//
LOCUS       DASZYN010000857         4545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_642346, whole genome shotgun sequence.
ACCESSION   DASZYN010000857 DASZYN010000000
VERSION     DASZYN010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642346"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2853)
                     /locus_tag="VHE33_21455"
     CDS             complement(<1..2853)
                     /locus_tag="VHE33_21455"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HVU00080.1"
                     /translation="MKQVFYDPKQRRRKILRRLTDVSIILLTIVGAVFVFSVLSKQTL
                     PELLLPAQRRNVKALKERQPELAKRAAARPARRRGRHHATDIVPNQDEGLRAAFYEND
                     EASYSALKAHIHEIDILFPDWLHVVSPDGNLVAATPLFPVHFYNVVDAAGVHGVDPEN
                     RVKTTIEAAKEETDVVPMLNDYDTLSQQWNGEVIGKMLHSAAARNRLHVQLDKFLFAN
                     PGYRGICLDFEMIPDEDQKLYAEWVAELHEDLHAKNLRIYVNVQASADDGTIRRLAQA
                     SDGIILMNYDEHEETSDPGPVASEPWFDGNLRRVLKLVPKEKVICGIGNYGFDWAQTL
                     PEKGNKASSKIVDVTDLSVQDAWQRAEDAGADVHLEGDELNPHFAYDDEDEHLRHQVW
                     FLDGVTALNELRAGRQMGLRTFALWRLGEEDPSLWQVWDRPGAANAPDLLKRVPPGQD
                     VDTEGEGDILRIVATPQAGERSILMDADNFTVIDENMVRLPRSYTIEQYGYDPHKLAL
                     SFDDGPDPVWTPKILDILKQYHVKGTFMVIGEEAQKNIGLLQRYVRDGNEIGNHTFTH
                     PDISEITPRQLELELNWTERLFAAETGRQPLYFRPPYSIDQEPDTNDEAAPAYRIQQM
                     GYTIIGNKIDTDDWSEHPHKSPQEITDSVLQQIEEMKTHPWMRGSVVLLHDGGGNRSA
                     TVAALPVLITTLRAKGYEFVPVSELMGKTTAEVMPPIAKDMRWQARIDWVAFFLFSFF
                     ANFVVDIFFIGDVLMSGRLILIGIFATIDRFRRRKIPGGVYEPGVAVLIPAYNEEKVI
                     VRTIRSVLKSDYANLRAIVIDDGSGDRTYDVAREAYPQEIEDGRLLVLTKANGGKAEA
                     LNYGLDSVEEEIYVGIDADTVIAHDAVSKLVRHFADERVGAVAGNAKVGNRINTWTRW
                     QALEYITSQNFERRAMDLFNVVTVVPGAIGAWRTAAV"
     gene            3070..4467
                     /locus_tag="VHE33_21460"
     CDS             3070..4467
                     /locus_tag="VHE33_21460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HVU00081.1"
                     /translation="MPSTAHRKWWLVLIALAAGVALRLWFIHAYPEIEGDPLVYGDIA
                     KNWMLHGIYGRTIDGHIVPTLIRLPGYPLFLILCFRLFGLEHYNAVMYAQVAMDLGTC
                     LLIADLARRIWSSRAGWCALWLAVLCPFTANYTATPLTETLELFTIALAFYAFERLLA
                     SPRWLWAFTLAFAWSYAAILRPDGALLAVALCPALIFYGSKRWGAPAMIRWALVCAFI
                     SILPFIPWTIRNARTFHVFQPLAPRYAVDPGEPTDPGFQRWTRTICVDFACTWEIYWN
                     ADNDVIDLANLPTRSYDSHQQHIETRALIDDYNRHTTITPEIDARFAAFAAQRIHDHP
                     FRYYVKLPIARLGDMILRPRTEMLWIELRWWQYRRHHAETVFAFGYAALNLAYFIAAL
                     IGFFRRPPLRGAILTFILLRCALLLTLEAPEQRYTLEFFPPLIALAAIALSGKQQPDT
                     TPSKQSASLADSLTR"
BASE COUNT          806 a         1672 c         1092 g          975 t
ORIGIN      
        1 gaccgctgcg gttcgccacg cgccaatcgc cccgggaacc accgtcacca cattgaacag
       61 gtccatcgcc cgccgctcga aattctggct cgtgatgtat tccagcgcct gccagcgtgt
      121 ccacgtgttg atgcggttgc ccaccttggc attgccggcc accgcgccca ctcgctcatc
      181 ggcgaaatgc cgcaccagct tcgaaaccgc atcgtgggcg atcaccgtgt ccgcatcgat
      241 gccgacgtaa atctcctctt ccacggagtc gagcccgtaa ttcagcgctt ccgccttgcc
      301 gccattcgcc ttcgtcagta ccagcaaccg cccgtcttca atctcctgcg gatacgcttc
      361 acgcgccaca tcgtaggttc ggtcccccga accatcatcg atcacgatcg cccgcagatt
      421 cgcataatcc gatttcagaa ccgagcggat cgtccgcaca atcaccttct cctcgttgta
      481 agcaggaatc agcacggcca cccccggttc atacactccg cccggaatct tcctcctccg
      541 gaaccggtcg atggtggcaa agatcccgat caggatcagg cgtccgctca tcagcacatc
      601 gccgataaag aagatgtcca ccacaaaatt cgcgaaaaac gagaacagga aaaacgccac
      661 ccagtcgatg cgcgcctgcc agcgcatatc cttcgcaatc ggcggcatca cctccgccgt
      721 cgtcttcccc atcagttccg acacgggcac aaattcataa cccttcgccc gcagtgtcgt
      781 gatcagtacc ggcagcgccg ccaccgtcgc cgatcggttc cccccgccgt cgtgcagcaa
      841 aacgacgctg ccccgcatcc acggatgcgt cttcatctcc tcaatctgct gcagaacgct
      901 gtccgtgatc tcctgcggcg acttgtgcgg atgctcgctc cagtcgtccg tatcgatctt
      961 gttgccgatg atcgtgtacc ccatctgttg aatgcggtac gccggtgctg cctcatcgtt
     1021 ggtgtccggc tcctgatcga tcgagtacgg cggccggaaa tacagcggct gccgtcccgt
     1081 ttccgccgca aacaagcgct ccgtccagtt cagctccagt tcgagctgcc gcggcgtaat
     1141 ttcactgatg tccggatgcg tgaacgtgtg gttgccgatt tcattgccgt cgcgcacgta
     1201 gcgttgcagc aatccgatgt tcttttgtgc ctcctctcca atcaccatga aggtgccctt
     1261 cacgtgatac tgcttcagga tgtcgaggat cttcggcgtc cacaccggat ccgggccatc
     1321 gtcgaaactc agcgccagct tgtgcggatc gtacccgtac tgctcgatgg tgtaagaacg
     1381 cggcagccgc accatgttct cgtcgatcac agtgaagtta tccgcgtcca tcaggatcga
     1441 gcgttcgccc gcttgcggcg tcgccacaat cctcaggatg tccccctcgc cctccgtgtc
     1501 cacgtcctgc cccggaggca cccgcttcag cagatccggc gcgttcgccg cgcccggccg
     1561 gtcccacacc tgccacagtg acggatcctc ttctcccagc cgccacaacg caaaggtccg
     1621 caaccccatc tgccgtccgg cgcgcagctc gttcagcgct gttacgccgt ccaaaaacca
     1681 cacctgatgc cgcaggtgtt cgtcctcatc gtcataagca aaatgcggat tcagctcgtc
     1741 gccttccaga tgcacatcgg cgccggcgtc ttccgccctc tgccatgcat cctgcaccga
     1801 taagtcggtg acgtccacga tcttgctcga cgccttgttg cccttttccg gcagcgtctg
     1861 cgcccagtcg aacccgtagt tcccgatgcc gcagatcacc ttttccttcg gcaccagctt
     1921 cagcacgcgc cgcagattgc catcgaacca gggttcactc gccaccggtc cgggatccga
     1981 cgtctcctcg tgctcgtcgt agttcatcag gatgatgccg tcgctcgcct gcgccagtcg
     2041 ccgaatcgtc ccatcgtcag cactcgcctg cacgttcaca taaatgcgca ggttcttggc
     2101 atgcagatcc tcgtgcaact cggcgaccca ctccgcgtac agcttctggt cctcgtccgg
     2161 aatcatctcg aaatcaagac agattccccg atatcccgga ttcgcaaaca aaaatttgtc
     2221 cagctggaca tggagccggt tccgcgccgc cgcactgtgc agcatctttc cgattacctc
     2281 gccattccat tgctggctca gcgtgtcgta atcgttcagc atcggcacaa catcggtctc
     2341 ttccttcgct gcctcgatcg tggtcttcac ccggttctcg ggatcgacac catgcacccc
     2401 ggccgcatcc accacgttgt aaaaatgcac cgggaacagc ggtgtcgccg ccaccaggtt
     2461 gccgtccggc gacacaacgt gcagccagtc cggaaacaga atgtcgattt cgtggatatg
     2521 cgccttcaac gctgaatagc tggcttcgtc gttctcgtag aacgcagcac gcagtccttc
     2581 atcctgattg ggcacgatgt cagttgcatg atgccggcct cgccgacgcg ctggccgcgc
     2641 cgcggctcgt ttcgccagct cgggctgccg ttctttcagc gccttcacgt tgcgccgctg
     2701 cgccggcagc agcagttccg gaagcgtctg cttcgacaga acgctgaaga cgaacaccgc
     2761 tcctacaatc gtcagcaaaa tgatcgatac gtcggtcagc cgccgcaaaa ttttgcgccg
     2821 ccgctgcttc ggatcgtaga aaacctgctt catcgtggcc tgcttcaatc ctacgatagg
     2881 aacgactcag cccttccgca cggacgtcac ctctgtgccg ctctgcctcg cctttcgctg
     2941 accgattctt agctaaccgg cctttagcta accggacctt agctgaccgg actttagctg
     3001 accggacttt cgctgaccgg actttagctg accgcttcat agcttccttt cccggatacc
     3061 atatccccaa tgccctctac ggctcaccgc aaatggtggc ttgtcctgat cgccctcgct
     3121 gccggagtcg ctctgcgcct gtggttcatc cacgcctacc ctgaaatcga aggcgatccg
     3181 ctcgtctacg gcgacatcgc caaaaactgg atgctccacg gcatctacgg cagaaccatc
     3241 gacggccaca tcgtcccgac tctcatccgc ctccctggct atcccctgtt tctgatcctc
     3301 tgttttcggc tcttcggcct cgagcactac aacgcggtca tgtatgcgca ggtcgccatg
     3361 gatctcggca cctgcctcct catcgccgat ctcgcccgcc gcatttggag ttcgcgcgcg
     3421 ggctggtgcg ctctctggct cgccgtgctc tgccccttca cagccaacta caccgcaacg
     3481 ccgctcacgg aaacgctgga actcttcacc atcgcgctcg ctttctatgc cttcgaacgc
     3541 ctgctcgcgt caccccgctg gctctgggct ttcaccctcg ccttcgcgtg gagctacgcc
     3601 gccatccttc gccccgatgg cgcgttgctg gccgtcgctc tctgtcccgc cctcatcttc
     3661 tatggatcaa aacgctgggg cgcgcccgcc atgattcgct gggcattggt gtgcgccttt
     3721 atctccatcc tgccgttcat tccctggacc attcgcaacg cccgtacctt ccacgtcttt
     3781 cagcccctcg ccccacgcta cgccgtcgat cccggcgaac ccaccgaccc cggcttccag
     3841 cgctggacaa gaaccatctg cgtcgatttc gcctgcacct gggagatcta ctggaacgcc
     3901 gataacgacg tcatcgacct cgccaacctc cccactcgct cctacgactc ccaccagcaa
     3961 cacatcgaaa cccgcgccct gatcgacgac tacaaccggc acaccaccat cacacccgag
     4021 atcgacgccc gctttgccgc cttcgccgcc cagcgcattc acgaccaccc attccgctac
     4081 tacgtaaagc ttcccatcgc acgcctgggc gacatgatcc tccgcccgcg caccgagatg
     4141 ctctggatcg agctccgctg gtggcagtac cgccggcatc atgccgaaac tgtatttgcc
     4201 tttgggtatg cagccctgaa cctggcttac ttcatcgctg ctctcatcgg attcttcagg
     4261 cgaccgcctc tccggggagc gatcctcacc ttcatcctgc tgcgctgcgc gcttctcctc
     4321 accctcgaag ccccagaaca gcgctacacc ctggaattct tcccgcccct catcgccctc
     4381 gccgccattg cgctcagcgg gaagcagcaa cccgatacaa caccgtcgaa gcaatcagca
     4441 tcactcgctg actcgctgac tcgctgactc gctgactcgc tgactcgctg actcgctgac
     4501 tcgctgactc gctgactcgc tgactcgctg actcgctgac tcgct
//
LOCUS       DASZYN010000858         3179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_893019, whole genome shotgun sequence.
ACCESSION   DASZYN010000858 DASZYN010000000
VERSION     DASZYN010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893019"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VHE33_21465"
     CDS             complement(<1..964)
                     /locus_tag="VHE33_21465"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HVU00082.1"
                     /translation="MARTAVPSAELKKPPEPASRNGNGSGPALSAEKLLEIYRLMVLS
                     RRTDDREIILKNQQKVFFQISGAGHEALLVAAGLSLRPGYDWFFPYYRDRALSLALGT
                     TVEEQFLQAVGAASDVASGGRQMPSHWSDPKLHIVSPSSSTATQCLQAVGCAEASLYL
                     TQHPDAAQPGESAQNDYRQFHAVDFHGDEVVYVSIGEGATSQGEFWESISIASNRKLP
                     LLFVVEDNGYAISVPVEVNTPGGNISRLLTSFPNFHFEEVDGTDPVACMKAFNTAVAH
                     CRARKGPALVHGHVIRPYAHSHSDDARLYRPESERDTEALRDPIQ"
     gene            1241..2425
                     /locus_tag="VHE33_21470"
     CDS             1241..2425
                     /locus_tag="VHE33_21470"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HVU00083.1"
                     /translation="MAAMAKPWYLRGPKRWQHFQGVPWKRLWLLMVAVFMLFSIFGLF
                     YDLMALGTVPYAVVAVDAIYSGLNAAMWILVLARLPMPFIGVLIVLQFFNAKVMTWFA
                     VSMTHAFRLTPVEEHTGVRVAALAMVGVVVTSYGFFVAFIRAEGRESYRLRTQLELAH
                     GIQKTLVPPIVLRTELFEVYGISEPSEKVGGDLVDAVLLPNGDVVAYLADIAGHGLSA
                     GILMGMLKTAARTALLDATTGHPSGTLPILLEKLNRVLPDVKEPQMYATLTAFRLNRD
                     GSAWYATAASPPVLHWSTRGRDARRIEEQQYPLGLLPVAGFTGEAIELSDGDLLVVAT
                     DGVLEVTDKAGQEFGVGRVEEVIAGGAQGALEGMANTILTRARAFGRQADDQTLLMIR
                     RL"
     gene            2539..2889
                     /locus_tag="VHE33_21475"
     CDS             2539..2889
                     /locus_tag="VHE33_21475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HVU00084.1"
                     /translation="MAKSYRDLDVWQCAIQLSVLLYKLTRDFPREEIYGLTSQLRRAG
                     VSIASNIAEGYGRSSRGEYRSFLGLARGSALEVQTQLVIARELGLGERSRMDEAEALA
                     ERAGKMLWSMREKL"
     gene            complement(2972..>3179)
                     /locus_tag="VHE33_21480"
     CDS             complement(2972..>3179)
                     /locus_tag="VHE33_21480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HVU00085.1"
                     /translation="LREMSRDRNKLRQKEIVEDTNHLMDLVRQLKDAVDKSNKDQLSL
                     SVVNTATEIEKLAKAVKEKMRDGQ"
BASE COUNT          590 a          810 c         1135 g          644 t
ORIGIN      
        1 tctggatcgg atcgcgcagc gcctcggtat cgcgctccga ttcgggccga tagaggcgcg
       61 catcatcgga gtgagagtgc gcgtaggggc gaatgacgtg gccgtggacg agcgcgggtc
      121 ccttacgggc gcggcagtgg gcgacggcgg tgttgaaggc cttcatgcag gccacgggat
      181 cggtgccgtc aacttcttcg aaatggaaat tcgggaacga cgtcagcagg cgcgaaatgt
      241 tgccgccggg cgtgttgact tcaaccggaa cggagatggc gtagccgttg tcttcgacca
      301 cgaagagaag cggcaatttg cgattggaag caatgctgat ggattcccag aactctccct
      361 gggaggttgc gccttcgccg atggagacgt aaacgacctc atcgccgtgg aaatcgacgg
      421 cgtggaactg gcggtaatcg ttctgagccg attcgcctgg ctgcgcggcg tcaggatgct
      481 gcgtgagata gagcgacgcc tctgcgcagc cgacggcctg caggcactgc gtcgcagtgg
      541 aggacgaggg cgagacaata tggagcttcg ggtcggacca gtgcgagggc atctggcgtc
      601 cgccgctggc gacgtcggag gcggctccga cggcctgaag gaattgctct tcgacagtgg
      661 tgccaagggc cagggaaagc gcgcggtcgc ggtagtaggg gaagaaccag tcgtagccgg
      721 gacggagcga aaggccggcg gcgacaagga gggcttcgtg accggcgccc gagatctgga
      781 aaaagacctt ctgctggttt ttgaggatga tctcgcggtc gtcggtgcgg cgcgagagca
      841 ccatgaggcg gtagatctcg agaagctttt ccgcagaaag ggcggggccg ctgccgttgc
      901 cgttgcggga ggcgggttcg ggcggctttt tcagttcagc gctggggaca gcggttcgcg
      961 ccatgcggac ctcattgctt ccttagatgt tcgctactcc agtttagaac ctggcgtggg
     1021 gattgaagaa accatgtcga atcagcacac aaagctgggg cgacgaaaaa ttgtatcgcg
     1081 tggagaggag gaaagcgcaa tggttggaag gtaacgaaga tgggtaccga gcgggggatt
     1141 cgcgaagagg aacagagcgc tgagcaccga aggacaggaa gggtcgatgc tggctgggtg
     1201 gcgctgtacg gaaagaggac ttcctggtat ggtgcagcct atggctgcga tggcaaagcc
     1261 ctggtattta cgtggaccga agcgatggca acacttccag ggcgttccct ggaagcggct
     1321 gtggcttttg atggtcgcag tatttatgtt gttcagcatc tttggcctgt tctatgacct
     1381 gatggcgctg ggcacggttc cctacgccgt tgtggcggtg gacgcgatct acagcggcct
     1441 gaacgcggca atgtggattc ttgtgctggc gcggctgccg atgccgttta tcggagtcct
     1501 gatcgttctg cagttcttca acgccaaagt gatgacgtgg ttcgccgtgt cgatgacgca
     1561 cgcgtttcgg ctgacgccgg tcgaagagca taccggtgtg agggtggctg cgcttgccat
     1621 ggtcggcgtg gtggtgactt cgtatggctt ttttgtggct tttattcgtg cggaaggccg
     1681 ggaatcgtac cggctgcgga cgcagctgga gctggcgcac ggaattcaga agacgctggt
     1741 gccgccgatc gtgctgcgga cggagttatt tgaggtgtac ggcatctccg agccgagcga
     1801 gaaggtgggc ggcgacttgg tggatgcggt gctgctgccg aatggcgacg tggttgccta
     1861 tctggcggac atcgcggggc atggactctc ggccgggatt ctgatgggca tgctaaagac
     1921 cgcggcgcgc accgcgctgc tcgacgcgac cacggggcat ccgagcggaa cgctgccaat
     1981 cctgctggag aagctgaatc gcgtgttgcc ggatgtgaag gagccacaaa tgtacgcgac
     2041 cctgacggcg ttccggctga atcgggacgg gagcgcatgg tacgccacag cggccagccc
     2101 gccggtgctg cactggagca cgcgcggacg ggatgcgcgg cggattgagg agcagcaata
     2161 tccgctgggg cttctgccgg tagcagggtt cacgggtgag gcgatcgagc tgagcgatgg
     2221 cgatctgctg gtcgtggcca ccgacggcgt gctggaggta acggacaagg ccgggcagga
     2281 atttggtgtc gggcgcgtgg aagaggtcat tgccggaggc gcgcagggag cgctggaggg
     2341 aatggcgaac accatcctga cccgggcgcg ggcgttcgga aggcaggccg acgaccagac
     2401 gttgctgatg attcgccggc tctgagctgc gggggcggcg cccggcgatc ggcgttcggc
     2461 gctcgggaaa gcccgagttc gcgtttggtg ttcggcgttc cgtaagctgt gaccggcatt
     2521 ctgcttcgga ggaaacaaat ggccaaatcc tatcgcgatc tggatgtttg gcagtgtgcg
     2581 atccaactct ctgtattgct ctacaagctg acccgggact ttccacgtga ggaaatctac
     2641 ggattgacga gccagcttcg cagggctggt gtctcgatcg cgagcaacat tgcggagggc
     2701 tacggccgct cttcaagagg ggaataccgg agttttctgg gattggcgcg tggttcggca
     2761 ttggaagtcc aaacgcaact cgtcatcgca cgcgaacttg ggctcggaga aaggagtcga
     2821 atggatgagg cggaagcact ggccgagcgt gccggcaaga tgctctggtc aatgcgagag
     2881 aagctgtaga cccaggcatt cggtgccctg tcctgattgc tttccggaag caggtgtctc
     2941 cgaacgctgc accccgaacg cggagtgccg cctattgccc atcgcgcatt ttttctttca
     3001 cggctttagc caatttctcg atttcggtgg cggtgtttac gactgaaagg gacagctggt
     3061 ccttgttgct cttgtcgacc gcgtccttga gctgtctcac caggtccatc agatgattcg
     3121 tatcctcgac gatttccttt tgacgtagct tgttacggtc acgcgacatc tcgcggagc
//
LOCUS       DASZYN010000859         2951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Acidobacteriaceae bacterium isolate SMAG_U6211
            k141_449409, whole genome shotgun sequence.
ACCESSION   DASZYN010000859 DASZYN010000000
VERSION     DASZYN010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957024
            Sequence Read Archive: SRR12170519
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidobacteriaceae bacterium (soil metagenome)
  ORGANISM  Acidobacteriaceae bacterium
            Bacteria; Acidobacteriota; Terriglobia; Terriglobales;
            Acidobacteriaceae.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 11:30:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,300
            CDSs (total)                      :: 4,236
            Genes (coding)                    :: 4,230
            CDSs (with protein)               :: 4,230
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 4 of 6
            Pseudo Genes (incomplete)         :: 5 of 6
            Pseudo Genes (internal stop)      :: 0 of 6
            Pseudo Genes (multiple problems)  :: 3 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Acidobacteriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449409"
                     /isolate="SMAG_U6211"
                     /isolation_source="soil"
                     /db_xref="taxon:2052142"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="29.64 N 82.34 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VHE33_21485"
     CDS             <1..346
                     /locus_tag="VHE33_21485"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HVU00086.1"
                     /translation="APFPDTKGKPTMQVLNADPKIGPGLLRIVMQPGDVIARHFHAGQ
                     AETLYILEGVFIDEGTAYPAGTELSVEPGAHHGPHTTSTGTTFLAMFTGNVNLTDFKL
                     SAGVEQLEAIAR"
     gene            396..1217
                     /locus_tag="VHE33_21490"
     CDS             396..1217
                     /locus_tag="VHE33_21490"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HVU00087.1"
                     /translation="MPAKRHPIVLIHGLWMTALCWENWVRRYSELGCEVIAKSWPGMD
                     VDIDDLRRDPSPIATLGINEIVEHYQAIIRGLDRPPIIIGHSFGGLIAQILLDRGLGS
                     AGVAIAPAPVKGILFLPFSTLKVSFPALSNPANNHRAVSLTPEQFHYAFTNNMSEEES
                     LKVFRRYAVPGPDHVLFQAAFANFNPHAATAVNFENNERAPLLLVSGGEDHVSPASVV
                     EANYKLYRKSTAVTEYLNFPRRTHYTLGQEGWEKVADSALEWAVAHAGSPATVTR"
     gene            1258..2193
                     /locus_tag="VHE33_21495"
     CDS             1258..2193
                     /locus_tag="VHE33_21495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HVU00088.1"
                     /translation="MKAGRIHRFGPPEVVVIDEIPRPELGDGELVVQTAFAGVAPWDA
                     LIRERKSVVNVKLPLILGSDLSGIVVSVGNGVTNYKPGDAIYGVTNKDFCGAYAEYAL
                     VSASMVAPKPRSLSFAEAASAPVVAVTAWQMLFEYAQLQSGQSVLIHGAAGNVGAYAV
                     QLARRAGLKIFATAGPDDLDHVQRAGTVINYKTDKFEDLVPSVDAVMDMIGGETQQRS
                     IAVLKPGGILVSVVSPFPKDTPVPSGIRTAFFLVDVTTARLNMLTQWFEAGELVPAVG
                     AVLPLEEARRANEMLAGLPHPKGKILLRMPNFAEP"
     gene            2260..>2951
                     /locus_tag="VHE33_21500"
     CDS             2260..>2951
                     /locus_tag="VHE33_21500"
                     /inference="COORDINATES: protein motif:HMM:NF005075.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HVU00089.1"
                     /translation="MDRLKGKRALITGGTTGIGFATAQEFLTEGARVIITGANQKNLD
                     AAKQKLGDSAVAIQADAGSVAAQDVVAAAVKRELGGLDVLFCNAGIVDMRPLDAWDEA
                     GFDRSFNTNFKGAFFLIQKLLPLFANPASIILNGSINAHIGMPNSSIYSATKAAMISL
                     TRTLSGELIGRGIRVNTVSAGPIETPLYAKLGMTEQGAAALLKQVPEGRFGDPKEIAK
                     AVVFFASDECSFS"
BASE COUNT          600 a          962 c          820 g          569 t
ORIGIN      
        1 ggcgcccttt cccgacacaa agggaaagcc cacgatgcag gtgctgaatg cagacccgaa
       61 gatcggcccc ggacttctgc gcatcgtcat gcagcccggc gacgtaatcg cccgccactt
      121 ccatgcagga caggcggaaa cgctctacat cctcgaaggt gtctttatcg acgaaggcac
      181 cgcgtatccg gccggaaccg agctgagcgt cgagcccggc gcgcaccatg gtccgcatac
      241 caccagcacc ggcaccacct ttcttgccat gttcactggc aatgtgaatc tcaccgactt
      301 caagctctca gcaggcgtgg aacagcttga agcgatcgct cgttagctgc cgtcgcaaga
      361 aaagaggttc aacaaaaaac aggagaaatt ggaaaatgcc tgccaaaaga caccccatcg
      421 ttctgatcca cgggctgtgg atgacagccc tctgctggga gaactgggtt cggcgctatt
      481 ccgagctcgg ctgcgaagtg attgcgaaga gctggcccgg catggacgtc gatatcgatg
      541 atctgcggcg cgatccgtct cccatcgcga cgctgggaat caacgaaatc gtcgagcact
      601 atcaagcgat catccgcggg ctcgaccgtc cgcccatcat catcgggcac tccttcggtg
      661 gtctcatcgc ccagattctg ctcgaccgcg gcctcggctc ggctggagtc gccatcgcgc
      721 ccgcgccagt gaaaggcatc ctgtttctgc ccttctccac gctcaaggtg tcgtttccgg
      781 ctctcagcaa tcccgccaac aatcatcgag ccgtgtctct gacacccgag cagtttcact
      841 atgcgttcac caacaacatg agcgaagaag aatcgctgaa ggtgttcagg cgctacgccg
      901 tccccggccc cgaccacgtc ctcttccagg cggctttcgc caacttcaac cctcacgccg
      961 ccaccgcagt caatttcgag aacaacgaac gcgcgccgct gctgctcgtc tccggcggtg
     1021 aggaccatgt atctccggcg tcggtcgtcg aggcgaacta caagctctat cggaaatcga
     1081 cagctgtgac tgagtacctg aacttccccc gccgcaccca ttacactctc ggacaggagg
     1141 gctgggagaa ggttgccgac agcgcgctcg agtgggccgt cgcccatgcc ggctcgccgg
     1201 ccactgtgac ccgatgattg tcccttcgca ggaccaggct ttatggttga ggacaagatg
     1261 aaggcaggca gaattcatcg ctttggtccg cctgaggtcg tggtgatcga tgagatcccg
     1321 cgacccgaac tgggcgacgg cgaactggta gtccagacag ccttcgccgg cgtcgcgccc
     1381 tgggatgctc ttattcgcga gcgcaaaagc gtcgtgaatg tgaagctccc gctgatcctc
     1441 ggctccgatc tgtccggcat cgtcgtctca gtcgggaacg gagtgacgaa ctacaagcca
     1501 ggcgatgcca tctacggcgt cacgaacaag gacttttgcg gagcctacgc tgaatacgcc
     1561 ctcgtctccg cctccatggt cgcgccgaag cccaggtcgt tgagttttgc agaagccgcc
     1621 agcgctcccg tcgtagccgt gaccgcctgg cagatgctct ttgaatacgc gcagctgcaa
     1681 tcgggtcagt cggtgctcat tcatggagcg gccggcaacg tcggcgccta tgcggtacag
     1741 ctggccaggc gcgcaggcct gaaaatcttc gcgaccgccg gtccggacga tctcgatcat
     1801 gtccagcgtg ccggcaccgt catcaactac aagaccgata aattcgaaga tctcgtccca
     1861 tcggtcgatg ccgtcatgga tatgattggc ggcgaaaccc agcagcgttc catcgccgtt
     1921 ctcaagccgg gagggatcct cgtctccgtc gtgtccccgt tcccgaagga caccccggtt
     1981 ccatcgggca ttcgaaccgc ctttttcctc gtcgacgtca caacggctcg cctgaatatg
     2041 ctgacccaat ggttcgaagc aggagaactg gttcccgccg tcggtgcggt tcttcccctg
     2101 gaagaggcgc ggcgtgccaa cgagatgctc gctggtttgc cccatcccaa agggaagatc
     2161 ctgctgcgga tgccgaactt tgctgagccc tagcctcttc gctgcctgga atctcggcgt
     2221 taatgactca ttgatcgcac ccatcgatag gagaaattca tggacaggct caaagggaag
     2281 cgggccctca tcaccggtgg aaccacgggc atcggattcg ctaccgcaca ggaattcctg
     2341 accgaaggcg cgcgagtgat catcacaggc gcgaatcaaa aaaatcttga tgccgcgaag
     2401 cagaagctcg gcgattccgc cgtggcgatc caggccgacg ccggcagcgt tgccgcacag
     2461 gatgtcgtcg ctgccgccgt gaagagggaa ctgggcggcc tcgacgttct cttctgcaac
     2521 gccggcatcg tcgacatgcg cccccttgac gcctgggacg aagcgggatt cgatcgctcc
     2581 ttcaatacca acttcaaagg cgcgttcttc ctgatccaga aactgctgcc gttgttcgcc
     2641 aatccggcgt ccatcattct caacggttcc atcaacgccc acatcggcat gcccaactcg
     2701 agcatctatt cggccaccaa agccgccatg atctcgctga cgcgtacctt atccggcgag
     2761 ctcatcggtc gcggaatccg cgtgaacacc gtcagcgccg gccccatcga gacgccgctc
     2821 tacgccaaac tcggcatgac cgagcagggc gccgctgcgt tgctcaaaca ggtccccgaa
     2881 ggccgctttg gcgatccgaa ggaaatcgcc aaagccgtcg tatttttcgc ctccgatgaa
     2941 tgcagctttt c
//
